LOCUS       DASRIQ010000001         6020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1042749, whole genome shotgun sequence.
ACCESSION   DASRIQ010000001 DASRIQ010000000
VERSION     DASRIQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042749"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFD06_00005"
     CDS             complement(<1..240)
                     /locus_tag="VFD06_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding-motility protein MglA"
                     /protein_id="HZN01944.1"
                     /translation="MTFINYAAREINCKLVYYGPGLGGKTTNVQYVYERSASPSKGKL
                     ISLATESDRTLFFDFMPLELGTIRGFRTRFHLYTVP"
     gene            complement(256..750)
                     /locus_tag="VFD06_00010"
     CDS             complement(256..750)
                     /locus_tag="VFD06_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZN01945.1"
                     /translation="MAGADLILHPEEYERIREVMARLRSECNAKMVFLIDKNGQQICA
                     QGETDAIDTTALASLAAGNVAATDSLARLIGEKEFSILFHEGERDNLHISVVAGRVIL
                     VVIFDERSSLGLVRLRVKRGSTDLRTVLETVWSRSAAPRKAAAGGSPFGEITDEDIDR
                     LFSD"
     gene            complement(886..1230)
                     /locus_tag="VFD06_00015"
     CDS             complement(886..1230)
                     /locus_tag="VFD06_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01946.1"
                     /translation="MALRTTPLTARERMILGWGKVRRFYLGAFRKEYVRKSLARRVGH
                     CNRTGACCRLMFTCPLLDQRSDPVRCSIHDIKPRNCNLFPIDERDLRDRDIIAPDSPC
                     GFSFRKESPTAP"
     gene            1333..1797
                     /locus_tag="VFD06_00020"
     CDS             1333..1797
                     /locus_tag="VFD06_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN01947.1"
                     /translation="MKTLELAVPPEPPAASDLDRVHSDSCPDEEHRILAVDDDEVILE
                     LMAETLRGQRYQVDVATSCQDALPLLMFRDYCGVILDLVLPDANGLSLYRQIARRKPM
                     MRPRVIFVTGAMDKREARRFVKLLDNPVLLKPFRLDDLVEAVRVLELPKGLK"
     gene            complement(1823..2116)
                     /locus_tag="VFD06_00025"
     CDS             complement(1823..2116)
                     /locus_tag="VFD06_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01948.1"
                     /translation="MGEVAFEYVVRTLQKTDETYLIEVERAPGHWMAMVRLPDGKYWN
                     MDRFDPRAPALPKQWVGGTAAAAADYAARVLINWFANERMEVVRRTYRLGEKG"
     gene            complement(2125..3843)
                     /locus_tag="VFD06_00030"
     CDS             complement(2125..3843)
                     /locus_tag="VFD06_00030"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN01949.1"
                     /translation="MRAGGALLLLLAVTPAAADVDTARIDAAFAEYARPGSPGCAVGV
                     VASGAIIFARGYGFASVENEVPITPRTIFDLGSTSKQITSAAVALLVLDGRLSLDDDI
                     RKYLPEIPVVRGSPAITVRRLLDHTSGLRDYTDLLSMEGYGETDLTTSAQGLAVLARQ
                     RGRNFPAGAEFRYTNSGHFLASILVQRVSGKSLRAFARERIFAPLGMTDSTYFDDHAL
                     VVPRRATGYAPREGGGFEVAMSDWEQVGDGGVQSSIEEMALWAANLDTGGKLGGRALV
                     DLLHTPGTLADGTPIPYGLGLFFGTYRGLRLVEHGGAWAGYRANLVRVPERRVAVITL
                     CNVSSAATRRLALAALDAALDALPESDRPPAAPASATVKASPATTGATPPAPASSRPE
                     TSFVGRYVSEHLGDVGRVELKPEGLFLEGEPLRPIGPGRFGLSSGNAVVTFSPEGTMT
                     VDDPRENPRPAVFKRLPPEPAGPSKKKSELAGRYRSADLDTGCLVAEAKDTLEFSCGG
                     GEKTAMSPLGGDLYNLGWGVLRVKRDERGAVESLLLTTRGLVEFRLGRTGGPAERGEG
                     RGSRGV"
     gene            complement(3840..4862)
                     /locus_tag="VFD06_00035"
     CDS             complement(3840..4862)
                     /locus_tag="VFD06_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209856.1"
                     /note="cyclodeaminase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZN01950.1"
                     /translation="MNPAGPAPGMLLLLREGAIRTLFDPRAGLEAIERAFAAYSTGKA
                     DLPSVIQLNVPEEGAEIHIKAGHLYGAPFWAVKVAGSFPNNPGRGLAASGGLVMAYDA
                     RTGAPAALLLDNGYITDLRTGLAGAVAARHLSRKDADVAAVLGTGSQARHQMRCLALV
                     RPLREIRIWGRHEDRARSLARELATEEGMRGIAVRAAASVEEAVRGAAIVVTTTSSRE
                     PILRSSWIGPGAHITAVGSDQPDKQEVEAALLARADLLVADSRPQCLRLGEIHHAVAS
                     GAIGPDDIDAELGEIVAGTKPGRPTDDAVTLCDLTGLGVQDVASANLILERARTTGAP
                     GETVPL"
     gene            complement(4885..>6020)
                     /locus_tag="VFD06_00040"
     CDS             complement(4885..>6020)
                     /locus_tag="VFD06_00040"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN01951.1"
                     /translation="SPANIAAPFRAVLRRQKKTRVVLGEVSGFDVTGRRVLLADGAAI
                     PYDSLVVASGSRHHYFGHDEWEKAAPGLKTVEDATAMRRRILLAFEQAERAPSPEERA
                     AWLTFVLVGGGPTGVELAGAIGELAHWTFRGNFRAIEPERARILLVEGNDRVLPAYPP
                     DLSQKAVRSLERLGAEVWTSALVTDVRPDGVTVRRDGNEERVGARTILWSAGVAASVL
                     GRRLAEACGFATDKVGRVPVGPDLTVPGHPEISVIGDLAVVRGKDGAPLAGVAQVAIQ
                     EGRYAARLIVAHLEQRTLPPFRYKDLGIMATIGRAAAVAVIGRVHLWGYPAWLAWLFI
                     HLMWLVQFESKLLVLVQWTWNYLTRNRSARLITGGPGDERIPA"
BASE COUNT          850 a         2134 c         2145 g          891 t
ORIGIN      
        1 gggaacggtg tagaggtgga agcgggtacg gaagccccgg atcgtcccca gctccagcgg
       61 catgaaatcg aagaacaggg tgcggtccga ctccgtggcc agcgagatca gcttcccctt
      121 ggagggcgag gcgctgcgct cgtagacgta ctggacgttg gtcgtcttcc cccccaggcc
      181 ggggccgtag tagaccagct tgcagttgat ctcgcgcgcc gcgtagttga tgaatgtcat
      241 gccggggtca ccgctctagt cactgaacag ccggtcgatg tcttcgtcgg tgatctcgcc
      301 gaagggggag ccgccggccg ccgccttcct cggcgcggcg gagcggctcc agacggtctc
      361 cagcacggtg cgcagatcgg tcgagccgcg cttgacgcgc agccgcacga gcccgagcga
      421 cgaccgctcg tcgaagatga cgaccaggat caccctcccg gccaccaccg agatgtgcag
      481 gttgtcccgc tccccctcgt ggaacaggat gctgaactcc ttctcgccga tgagccgcgc
      541 cagcgagtcg gtcgccgcga cgttgccggc ggcgagggac gccagcgccg tcgtgtcgat
      601 ggcgtcggtc tccccctggg cgcagatctg ctgcccgttc ttgtcgatca ggaagaccat
      661 cttggcgttg cactcggacc tcaggcgggc catcacctcg cggatgcgct cgtactcctc
      721 ggggtgaagg atcaggtcgg cgccggccat cgagtcgcaa ccctccagcg gggcccggat
      781 gggtgtgacg acggaccgga ccggcttcca atatggggag cgacggagcg aaagtccatc
      841 gaccccgggg tttggagccg ttccgcccgc ccccccggcc gggcgtcagg gagccgtcgg
      901 gctctccttc cggaaggaga agccgcacgg cgaatcgggc gcgatgatat cgcgatcgcg
      961 caggtcgcgc tcgtcgatcg ggaagaggtt gcagttgcgc ggcttgatgt cgtggatgct
     1021 gcagcgcacc ggatcgctgc gctgatcgag gagcgggcag gtgaacatca gccggcagca
     1081 ggctcccgtg cggttgcagt ggccgacgcg tcgggcgagg cttttgcgga cgtactcctt
     1141 gcggaaggcg ccgaggtaga agcggcgcac cttcccccag ccgaggatca tccgctcgcg
     1201 ggcggtcagc ggggtggtgc gcaacgccat gacgttttcg tccttcctct tgggtgaatc
     1261 tgccgttgca attccgtcat tcccggctcc gccgcttgcg gcgcgggttt ccgggcccta
     1321 ccttcacgag tgatgaagac tctcgagctc gccgtgccgc cggagccgcc ggccgcgtcc
     1381 gacctggacc gcgtccattc cgattcctgt cccgacgagg agcaccgcat cctggcggtg
     1441 gacgacgacg aggtcatcct cgagctgatg gccgagacgc tgcgcggcca gcgctaccag
     1501 gtcgatgtcg ccacctcctg ccaggacgcc ctgccgctcc tgatgttccg ggactactgc
     1561 ggcgtcatcc tcgatctcgt cctgccggac gccaacggcc tgtccctcta ccgccagatc
     1621 gcccgccgca agccgatgat gcggccccgg gtgatcttcg tgacgggcgc gatggacaag
     1681 cgcgaggcgc gtcgcttcgt caagctcctc gacaacccgg tcctcctgaa gccgttccgg
     1741 ctcgacgacc tggtcgaggc ggtgcgcgtc ctggagctgc cgaagggcct caagtagccc
     1801 cggccgggcc gcgcgaccgg cgtcagcctt tctcccccag cctgtaggtc ctgcgcacga
     1861 cttccatgcg ctcgttcgcg aaccagttga tcaggacgcg ggcggcgtag tcggccgccg
     1921 cggcggcggt gccgccgacc cactgcttcg ggagggcggg cgcgcgcggg tcgaagcggt
     1981 ccatgttcca gtacttgccg tccggcaggc gcaccattgc catccagtgt ccgggcgctc
     2041 tctcgacctc gatgaggtag gtttcgtcgg ttttctggag ggtgcgaacc acgtactcga
     2101 aggccacttc acccatgcgc gtgattatac accgcgggat ccgcgtcctt ccccgcgttc
     2161 cgcgggtcct ccggtgcgcc cgagccggaa ctccacgagg ccgcgcgtgg tgagcaggag
     2221 cgactcgacg gcgccccgct cgtcccgctt cacccgcagg accccccagc cgagattgta
     2281 aagatcgccg ccgagcgggc tcatcgccgt cttttcgccg ccgccgcagg agaattcgag
     2341 agtgtccttg gcctcggcga cgaggcagcc cgtgtcgagg tcggcgctgc gatagcggcc
     2401 ggcgagctcg ctcttcttct tcgacggccc ggccggctcg ggcgggagcc gcttgaagac
     2461 ggccgggcgg gggttttcgc gcgggtcgtc caccgtcatc gtcccctccg gggaaaaggt
     2521 cacgacggcg ttcccggaag agagaccgaa ccggcccggc ccgatcggcc gcagcggctc
     2581 cccttcgagg aagagccctt caggcttgag ctcgacacgg cccacgtccc ccaggtgctc
     2641 gctgacgtaa cgccccacga acgaggtctc cggccgggag gaggccggcg cggggggcgt
     2701 ggctcccgtg gtggcggggg aggccttgac cgtcgcggag gcgggagccg cggggggccg
     2761 atcggattcc ggcagggcgt cgagcgccgc gtcgagggcg gcgagcgcca gccgccgggt
     2821 cgcggcggac gagacgttgc acaaggtgat cacggcgacg cggcgctcgg ggacgcgcac
     2881 gaggttggcg cggtagccgg cccaggcgcc gccgtgctcc acgagcctca gtccccgata
     2941 cgtcccgaag aacaggccga gtccgtaggg aatcggggtg ccgtcggcga gcgttccagg
     3001 cgtgtggagc aggtcgacga gcgcgcgccc cccgagtttc ccgcccgtgt cgaggttcgc
     3061 cgcccacagc gccatctcct cgatcgacga ctgcacgccg ccgtccccca cctgctccca
     3121 gtccgacatc gccacctcga acccgccccc ctcgcgcggc gcgtagccgg tggcgcggcg
     3181 cggcacgacg agcgcgtggt cgtcgaagta ggtgctgtcg gtcatcccga gcggcgcaaa
     3241 gatccgctcg cgggcgaagg cgcgcaggct cttccccgac acccgctgga cgaggatcga
     3301 agcgaggaag tgccccgagt tcgtgtagcg gaactccgcc ccggcgggga agttgcgccc
     3361 tctctgccgg gcgaggacgg cgagcccctg ggcgctcgtc gtcaggtcgg tctcgccgta
     3421 cccctccatc gaaaggaggt cggtgtagtc gcgcaggccg ctcgtgtgat ccaggagccg
     3481 ccgcacggtg atcgccggcg atcccctcac caccggaatc tccggcaggt acttgcggat
     3541 gtcgtcgtcg agcgacagcc ggccgtccag gacgaggagc gcgacggcgg cggaggtgat
     3601 ctgcttcgag gtcgagccga ggtcgaagat cgtccgcggc gtgatcggca cctcgttttc
     3661 gacgctcgcg aagccgtagc cgcgcgcgaa gatgatcgcc cccgacgcga cgacgcccac
     3721 cgcgcagccg ggggatccag gacgggcgta ctcggcgaag gcggcgtcga tccgcgcggt
     3781 gtcgacgtcc gcggcggccg gtgtcaccgc cagcaggaga agcagggcgc ctccggctct
     3841 cacagcggga ccgtctcgcc cggagcgccg gtggttcggg cgcgctccag gatgaggttc
     3901 gccgaggcga cgtcctgaac gccgaggccg gtcaggtcgc acagggtcac ggcgtcgtcg
     3961 gtggggcgtc ccggcttcgt cccggcgacg atctccccca gctccgcgtc gatgtcgtcg
     4021 gggccgatcg cccccgaggc gacggcgtga tggatctctc ccagacgcag gcactgcggt
     4081 cggctgtcgg ccacgagcag gtcggcgcgc gccagaaggg ccgcctcgac ctcctgcttg
     4141 tccggctggt ccgatccgac cgcggtgatg tgggcgcccg gcccgatcca cgaggacctc
     4201 aagataggct cgcggctgga ggtggtggtg accacgatcg cggctccgcg gaccgcctcc
     4261 tcgacgctcg cggcggcgcg cacggcgatg ccgcgcatgc cctcctcggt cgcgagctcg
     4321 cgcgcgaggg agcgcgcccg gtcctcgtgc cgcccccaga tgcgtatttc gcgtagcggc
     4381 cggacgagcg ccaggcagcg catctggtgg cgcgcctggg atcccgtgcc gagcaccgcc
     4441 gcgacgtcgg catccttgcg gctcaggtgg cgggccgcga ccgctcccgc gagcccggtc
     4501 ctcaggtcgg tgatgtagcc gttgtcgagc aggagcgccg cgggggcgcc ggtgcgcgcg
     4561 tcgtacgcca tgaccagccc gccgctcgcc gcgaggccgc gcccgggatt gttcggaaag
     4621 gaaccggcca ccttcaccgc ccagaacggc gcgccgtaga ggtgccccgc cttgatgtgg
     4681 atctccgccc cctcttccgg aacgttgagc tggatcaccg acggcaggtc ggccttgccg
     4741 gtcgagtagg cggcgaaggc gcgctcgatc gcctcgagcc cggcgcgcgg gtcgaagagg
     4801 gtgcggatcg ccccctcgcg caggaggagg agcatcccgg gggccgggcc ggcggggttc
     4861 acgtggtggt cgcctggagc ggagtcacgc ggggattctc tcatcacccg ggccgccggt
     4921 gatgagccgg gcggagcggt tgcgggtcag gtagttccag gtccactgca cgagcacgag
     4981 gagcttgctc tcgaactgca ccagccacat caggtggatg aagagccagg cgagccaggc
     5041 cggatacccc cacaggtgca cccggccgat cactgccacc gccgcggccc ggccgatggt
     5101 cgccatgatg ccgaggtcct tgtaccggaa cggcggcagg gtgcgctgct cgaggtgcgc
     5161 cacgatgagc cgcgccgcat agcgcccctc ctggatcgcc acctgcgcca cgccggcgag
     5221 cggcgctcca tccttgccgc ggacgacggc cagatcgccg atgacggaga tctccggatg
     5281 ccccggcacg gtcagatcgg ggccgaccgg gacgcgcccg accttgtcgg tcgcgaatcc
     5341 gcacgcctcc gccaggcgcc ggcccagcac ggaggcggcc acccccgccg accacaggat
     5401 ggtccgggcg ccgacccgct cctcgttccc gtcgcggcgg acggtgaccc cgtcgggccg
     5461 cacgtccgtc accagggcgg aggtccagac ctccgccccc agccgctcga gcgaacggac
     5521 cgccttctgc gacaggtcgg gcggatacgc cgggagcacc cggtcgttcc cctcgacgag
     5581 gaggatgcgg gcccgctccg gttcgatcgc gcggaagttg ccgcggaagg tccagtgggc
     5641 gagctccccg atcgccccgg cgagctcgac gccggtgggg ccgcctccca cgaggacgaa
     5701 ggtgagccag gccgcccgct cctccgggct cggtgcccgc tccgcctgct cgaacgccag
     5761 gaggatccgt cgccgcatgg cggtggcgtc ctcgaccgtc ttgagccccg gcgcggcctt
     5821 ctcccactcg tcatggccga agtagtggtg ccgcgagccg ctggcgacga cgagcgagtc
     5881 gtacgggatc gccgcgccgt cagcgagcag cacgcgcctc ccggtcacgt cgaacccgga
     5941 gacctcgccg agaacgaccc gggttttctt ctgccggcgc agcaccgccc ggaacggcgc
     6001 ggcgatgttg gccggggaga
//
LOCUS       DASRIQ010000002         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1304000, whole genome shotgun sequence.
ACCESSION   DASRIQ010000002 DASRIQ010000000
VERSION     DASRIQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304000"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2324)
                     /locus_tag="VFD06_00045"
     CDS             complement(<1..>2324)
                     /locus_tag="VFD06_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN01952.1"
                     /translation="LLFGGRPQPLEDSGPDDPVERAMQAAQARVLDRLRPVEAATSPA
                     EFSPLGGPLLGTVLQGEAAKGMLLTSQGHVDWDADRDAVAAKIPAALRLSAGESRRFG
                     HGTLHPGLNYLRLSEAEVSRRGLDAVQSEVARHARILGWLPERTVLAWVEPRDLQALG
                     DTAGVEKSRALEPYQKIAPLLGTMPRLSRREAANPDLLSRVTFVPGRGGAAAIEAIGR
                     MPGVSEVAPDATDDNGVQLRVRFDRVVDLARRDEVLAIEPVPDFMLANAENVPTIQAG
                     SAEDARFVRPFDDAGVDGGGLDTSGDGMRLNDGSDLVPPQIVAITDNGISYDTPSFAQ
                     TMTGTGVLGPAHRKIHAIQNVTDSGTSCDAPLSGSGTHGNVVASAIAAYPSALGAFAS
                     RPGNGGPTDPRNENLDGVARGARIIVQDAGTTASCTINTLVEKGGNVTPGSLLDRLNT
                     AVSAGGTQVHLTVFPFGTPNFSNVVLSIVLGQYPAEAAQIDTFLYNNRDYMVFVPAGN
                     NGGVMGTGRLGLMLRVIPDLFNGTAIDENPNFQSPIQVSPPATAKNVVGVGAGTSDCF
                     TFFGPTDCEASIANFTSRGPATSQSLRMAPMLTAPAFDLSVGPYTTGVAVFRSTDNDN
                     LVPIETQLDEGNSGTSFAAAYLTGAAAVLRDYFAQGFYPTGSRGTAADRMPGVSGALV
                     KAALAASADFNEGGIATQGQDNNERNLRRTRCLDLGTVTGIGGSSVQVGIMCNSEQGY
                     GRPILTNVLPLADWPDQFVHHPDSLLPREYPAAG"
BASE COUNT          311 a          814 c          857 g          342 t
ORIGIN      
        1 gccccgcggc cgggtattcg cgcggcagga gagagtcggg gtggtgcacg aactggtccg
       61 gccagtcggc gagcggcagg acgttcgtca ggatcgggcg gccgtacccc tgctcgctgt
      121 tgcacatgat cccgacctgc acggaggagc cgccgatgcc ggtcacggtg ccgaggtcga
      181 ggcagcgggt gcggcgcagg ttgcgctcgt tgttgtcttg gccctgggtc gcgatccccc
      241 cttcgttgaa gtccgccgag gcggcgaggg ccgccttgac gagcgccccg gacacccccg
      301 gcatccgatc ggccgccgtg ccgcggctgc cggtcgggta gaacccctgc gcgaagtagt
      361 cgcgcaggac cgccgcggcg ccggtcaggt aggcggcggc gaaggaggtt ccgctgttcc
      421 cctcgtcgag ctgcgtctcg atcgggacca gattgtcgtt gtcggtgctg cggaacaccg
      481 ccacgccggt cgtgtagggg cccacggaga ggtcgaacgc cggggcggtc agcatcggcg
      541 ccatgcgcag cgactgcgag gtcgcggggc cgcgcgaggt gaagttggcg atgctcgctt
      601 cgcaatcggt cggtccgaaa aacgtgaagc aatcggatgt ccccgccccg acgccgacga
      661 cgttcttggc ggtcgccggc ggcgacacct ggatcggcga ctggaagttc gggttctcgt
      721 cgatggcggt gccgttgaac aggtccggga tgacccgcag catcagcccg aggcggccgg
      781 tgcccatgac gccgccgtta ttcccggccg gcacgaagac catgtagtcg cggttgttgt
      841 agaggaaggt gtcgatctgc gccgcctcgg ccgggtattg ccccaggacg atcgacagga
      901 cgacgttgct gaagttcggc gtgccgaacg ggaacacggt caggtggacc tgcgttccgc
      961 cggcggagac ggccgtgttc agccggtcga gcagcgaccc cggcgtcacg ttgccgccct
     1021 tctccacgag cgtgttgatc gtgcaggagg cggtcgtccc ggcatcctgg acgatgatgc
     1081 gggcgccgcg cgccacgccg tcgaggttct cgttgcgcgg atcggtcggg ccgccgttgc
     1141 cgggccggct cgcaaacgcg ccgagggcgg acgggtacgc cgcgatggcg ctggcgacga
     1201 cgttgccgtg cgtgccgctc cccgacaggg gcgcatcgca cgaggtgccc gaatcggtga
     1261 cgttctggat cgcgtggatc ttccggtgcg ccggaccgag cacgcccgtg ccggtcatcg
     1321 tctgggcgaa cgacggcgtg tcgtaggaaa tgccgttgtc ggtgatggcg acgatctgcg
     1381 gcggcacgag atcgctgccg tcgttgaggc gcatgccgtc gccgctcgtg tcgaggccgc
     1441 cgccgtccac gcccgcgtcg tcaaatgggc gcacgaacct ggcgtcctcc gccgacccgg
     1501 cctggatcgt cgggacgttc tcggcgttgg ccagcatgaa gtcggggacc ggctcgatcg
     1561 ccagcacctc gtcgcggcgg gccaggtcga cgacgcgatc gaagcgcacc cgcagctgca
     1621 ccccgttgtc gtcggtcgcg tccggggcga cctcgctcac ccccggcatc cgtccaatcg
     1681 cctcgatcgc cgcggcgcca ccgcggccgg ggacgaaggt cacgcgcgac agcaggtcgg
     1741 gattggccgc ctcgcggcgg ctcaggcgcg gcatcgtccc gaggagcggc gcgatcttct
     1801 ggtagggctc gagcgcgcgg ctcttctcca cgccggccgt gtcgcccagc gcctggagat
     1861 cgcggggctc gacccacgcg aggaccgtcc gctccggcag ccacccgagg atgcgggcgt
     1921 gccgggccac ctccgactgc accgcgtcca gcccgcgacg cgacacctcg gcctccgaca
     1981 ggcgcaggta gttcaggccc ggatggagcg tcccgtgacc gaagcgccgc gactcgccgg
     2041 ccgagagccg gagcgccgcg gggattttcg cggcgacggc gtcgcggtcc gcgtcccagt
     2101 ccacgtgccc ctggctcgtc aggagcatcc ccttcgccgc ctccccctgg aggaccgtcc
     2161 cgaggagcgg cccgcccagc ggcgagaact ccgccggcga cgtcgccgcc tccaccggcc
     2221 tgaggcgatc caggacacgc gcctgcgccg cctgcatggc ccgctcgacc ggatcgtccg
     2281 ggccggagtc ctcgagaggc tggggccggc cgccgaacag cagg
//
LOCUS       DASRIQ010000003         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_522578, whole genome shotgun sequence.
ACCESSION   DASRIQ010000003 DASRIQ010000000
VERSION     DASRIQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522578"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFD06_00050"
     CDS             complement(<1..266)
                     /locus_tag="VFD06_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01953.1"
                     /translation="MIDEAVVPRQGNLPSEARAASPEENRTPEASPAPEEETPAGDDT
                     TSDRTPPVLGQLRFEPAVVEGGSATTLTIQATDSLSGVKSARGE"
     gene            complement(686..2245)
                     /locus_tag="VFD06_00055"
     CDS             complement(686..2245)
                     /locus_tag="VFD06_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZN01954.1"
                     /translation="MIAIVFPLWAAVVVPPANDLPVAGRVEMRLEIVADRRDGPAESC
                     EAIQTRSATGPVSFAFWGTLAEPGASGRCGAGVVPDDMTSNSTTPSAETPALLLIHEV
                     SADVGWDAGTMDPGPVGTFQVFLTLRTRQSIALSKDDRPQFGAPVIDRRSIRLEPGDE
                     FVVPLAVDARGREALGVHQVLVRVRAGWAGREGATEYGALAVTGAMPGSEVALDGGLA
                     GHAGPDGGLRLSRVPVGQREVRLRGSSGPVVSRTVLLVKGRTVQVSPEAADGGSASQP
                     SLEATGKNAEGFQEYRRVRDGATMVEIPEGEFLMGNLETEGSPQPHTVFVSKFLMDKL
                     PLTVGRYRRFATATGRPLPPDPYWGVHEDHPVAFVRWDEAKAYCEWAGGRLPTEAERE
                     KATRGTDGRLFPWGSEPPSPERAVFRRNWGQQGNDTAGVRPAGAGPYGLLDTGGNMWE
                     YCEDWYSPDYYASSPKIDPAGPKTGRARIVKGGSWDSRPTVLSASSRNFAYIGYREGD
                     FGFRCAANPPR"
BASE COUNT          359 a          911 c          711 g          369 t
ORIGIN      
        1 atttctcccc gggccgactt gacgcccgac aggctgtcgg tcgcctggat cgtgagcgtc
       61 gtcgcgcttc cgccttcgac gaccgccggc tcgaagcgga gttgaccgag gaccggcggt
      121 gtgcggtccg aggtcgtatc gtcgccggca ggtgtttcct cctccggtgc ggggctcgct
      181 tccggcgtgc ggttctcttc cggcgatgcg gcccttgctt cggaaggaag gttcccctgc
      241 cgtggaacga ccgcttcgtc gatcaccggc tgcgacgctc cagaggcggt cggattcgtg
      301 agatccgcgc cacctcgaga acccggcgct tccggcggca aaccggccgc caggggcggc
      361 gagggatggt gcgctccgcc tcgaacgtca ccttcccccc gtgtctccgg gcggtcctca
      421 ccattcgtcc ccgtctgtcc cgcctcggga cccgcgtcac gatgtccgct ctcgcccgtc
      481 ggcacggcgc ctcccgatgg aaccggatcg gccgatgaaa cgtgagccgc cacgccggat
      541 tcgacggcgc gacggcgcgt ctcgagccac ctccacacat tgatcgcgcc gaggatcaag
      601 caggccaggg cgagagcggc aatcgtcagg ccgtatcccc accgcttcga ttcatcccga
      661 atcatgaact ctccccgcgc tcccatcagc gaggcggatt cgccgcgcag cggaacccga
      721 agtccccttc gcggtagccg atgtaggcga aattccggct cgacgcgctc aacaccgtcg
      781 ggcggctatc ccaggagcct cccttgacga tgcgcgcccg gccggtcttc ggaccggcgg
      841 ggtcgatctt cgggctcgac gcgtagtaat cggggctgta ccaatcctcg cagtactccc
      901 acatatttcc gcccgtgtcg agcagaccat aggggccggc gcccgccggc cggactccgg
      961 ccgtgtcgtt cccctgctgg ccccagttgc ggcgaaacac agcgcgttcg ggggacggcg
     1021 gctcgcttcc ccaggggaac aggcggccat cggttccgcg cgtcgctttc tcccgctccg
     1081 cctccgtcgg cagacggccc cccgcccact cgcaatacgc cttcgcctcg tcccacctca
     1141 cgaacgccac cggatggtcc tcgtgcacgc cccagtacgg atccggcggc aacggtcgcc
     1201 ccgtcgccgt cgcgaaccgc ctgtaccgtc ccaccgtcaa cggcagcttg tccatcaaaa
     1261 acttcgagac gaacaccgtg tgcggctgcg gcgacccctc ggtctcgaga ttccccatca
     1321 ggaactcccc ttccgggatc tccaccatcg tcgccccgtc ccgcacccgc cggtactcct
     1381 ggaagccttc cgcgttcttt cccgtggctt ccagagaggg ctgcgacgcc gagccgccgt
     1441 ccgcggcctc gggcgacacc tgcaccgttc tccccttcac gagcagcacc gtccgggaga
     1501 ccaccggccc cgatgagccc cgcagacgca cctcccgctg tccgaccggc actctcgaga
     1561 gccgcaggcc cccgtcgggc ccggcgtgac cggcgagtcc accgtccaga gccacctccg
     1621 accctggcat cgctcccgtc acagccaggg cgccgtactc cgtcgccccc tcgcgacccg
     1681 cccagccggc ccggacgcgg accagtacct gatggacccc cagagcctcg cgtccgcgcg
     1741 catcgaccgc gagcggcacg acgaactcat cccccggctc gagacggatg gaccggcgat
     1801 cgatcaccgg agcgccgaac tgcggccggt cgtccttcga caacgcgatc gattgccgcg
     1861 ttctcagcgt gagaaagacc tggaacgttc ccacaggacc ggggtccatg gttcccgcgt
     1921 cccagccgac atctgccgag acttcatgga tcaggagtag cgccggggtt tcggcggaag
     1981 gcgtcgtcga attgctcgtc atgtcatccg gcacaacacc agccccgcac cggcccgatg
     2041 cgcccggctc cgccagagtg ccccagaagg cgaacgacac cggaccggtc gcgctcctgg
     2101 tctggatggc ctcgcacgat tccgccgggc cgtccctccg gtcggccacg atttcgagac
     2161 gcatctcgac gcgtcccgcg acaggaaggt cgttcgcagg aggcacgacc acggccgccc
     2221 acaaggggaa aacgatcgcg atcatcgtcc gtccgttttc cgatcctcct gaacgcaccg
     2281 gaaccccagc atggggccgt tccaggctgg ctcgctcccc agtcgggatg cgccgcggac
     2341 cagaaaggaa
//
LOCUS       DASRIQ010000004         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2942, whole genome shotgun sequence.
ACCESSION   DASRIQ010000004 DASRIQ010000000
VERSION     DASRIQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2942"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..1159)
                     /locus_tag="VFD06_00060"
     CDS             complement(269..1159)
                     /locus_tag="VFD06_00060"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HZN01955.1"
                     /translation="MGADGTVGAVAGLARFPVKSMGGERLEEAELTERGIVGDRAFAI
                     IDVETGKVASAKSVRLFPHLMRCQASYVESPRAGRELPPVRITLPDGRGVTSDSPDVD
                     RTLSAGFGRAVRLAGSAPEDFTIDQYHPDIEGADPAGRRDQVVEQKLGSAYFDALGVE
                     SPVPVGAFFDLYPMSLLTTSTLARLAELAPAVRFDLRRFRMNVIVDSREAGFLENGWI
                     GREVALGDTVRLRVAKHDLRCVMTTLAQDELPNEPEVLRALVRHNPIQVAKAAYPCAG
                     VYAVVGTPGTLRVGDSVILV"
     gene            1371..2558
                     /locus_tag="VFD06_00065"
     CDS             1371..2558
                     /locus_tag="VFD06_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01956.1"
                     /translation="MLAFGVTLANPVRASDVGYIYGRVETVGGGAYEGQLRWGTEESF
                     WDDIFNATKVENENLDYVDRKVLERGRWRHWSNWDFFGNRDHTHVFAIRFGDLKRLHV
                     RGDDALVVEFRNGESLRLGGGSNDVGADITVVDPKLGKHTVKWDRIRTIEFRETPAKL
                     PAKLGEPIYGTVKSGRYDFVGRIQWDNDEDLTGDKLDGETQDGKVSVAFGDIAAIRKH
                     RQGALVRLKSGSELYLTGTNDVNHGNRGVVVVVARLGAVKIGWDDFDEVTFAPAPNSG
                     RGYAEYAQASDLSGEVTTRDGRYDGRIVFDLDESRDFELLQGTNGDTEYLIPFRDIAR
                     ITPQGRFRSEVVLHMGMTIELEDSQDVTRRNDGLLVFTGERKPKYVAWQDVTDVRFEP
                     PPR"
BASE COUNT          480 a          915 c          966 g          439 t
ORIGIN      
        1 agcccgggcc gtgcggggcc cgtgcggccg cggccgaggg cgtgctgggt ggaatcgtcg
       61 actcgcccgg gcgcacggcc cggcggccgg aggtgatctc gggttcgggc tcgggaagcg
      121 cggaggacat gagggaagga ggctccaggt cgggtgaggt cgcgggccgc cccgcgagct
      181 cgactcatcg cggtatcggc cttggacgcc cgccgcatga gcgcccccgg ctcgctgcac
      241 gaacgaggca gacccagggc gcgggcggct agacgaggat cacggagtct cccacgcgta
      301 gcgtgcctgg tgtcccgacg accgcgtaca cccccgcaca gggataagcg gccttggcga
      361 cctggatggg gttgtggcgc accagggcgc gcaggacttc cggttcgttc gggagctcgt
      421 cctgggccag cgtcgtcatg acgcaacgca aatcgtgctt cgcgacccgg agacggaccg
      481 tgtcgcccag cgcgacttcc cggccgatcc agccgttctc cagaaaccct gcctcacggc
      541 tgtcgacgat cacgttcatg cggaaccggc gcaggtcgaa tcggaccgcg ggggcgagct
      601 cggcgagccg tgcgagcgtg gacgtcgtca gcagggacat ggggtagagg tcgaagaacg
      661 ccccgacggg caccggggac tcgacgccga gcgcgtcgaa gtaggccgag ccgagcttct
      721 gctccacgac ctggtcccgg cgcccggccg gatcggcgcc ctcgatgtcg ggatggtact
      781 ggtcgatggt gaagtcctcc ggggccgaac cggccagccg caccgcgcgc ccgaagccgg
      841 ccgagagcgt tcgatcgacg tcgggggagt cgctggtcac cccccgaccg tccggcagcg
      901 tgatccgcac gggtggcagc tcgcggccgg cacgtggcga ttcgacgtag ctcgcctggc
      961 agcgcatcag gtgaggaaag agccggacgc tcttggcact cgcgaccttg ccggtttcga
     1021 cgtcgatgat cgcgaaggcg cgatcgccca cgatccctcg ctcggtgagc tctgcctcct
     1081 ccaaccgctc gccgcccatg gacttgacgg gaaaccgggc caatcctgcc acggcgccga
     1141 ccgttccgtc tgcccccatg ctctcctccg gccgttgggc ccatgaaggg tgccgttcat
     1201 tggagctcga atccgaacgg tgcactatag gcgcttcggg tccggtggcg ggaggtctgc
     1261 ctgcaacaaa acgggcgccc cccacgttct aggaccatgc tccccgcagt cgcttgggca
     1321 ggcggtcatc cacagggagg ttcgtcgtga acgcaaggct cccgatcgcg gtgctggcct
     1381 tcggggttac cctggccaac ccggtccgcg cttcggacgt gggctatatc tacggacggg
     1441 ttgaaaccgt aggcggaggg gcgtacgagg ggcagcttcg ctgggggacc gaagagtcgt
     1501 tctgggacga catcttcaac gccaccaagg tcgagaacga gaacctcgac tacgtcgacc
     1561 gcaaggtgct cgagcgtggg agatggcgcc attggagcaa ttgggacttc ttcgggaatc
     1621 gcgaccatac ccacgtgttc gcgattcgct ttggagactt gaagcgcctc cacgtgaggg
     1681 gtgacgacgc cctggtcgtc gaattccgaa acggtgagag cctgaggctg ggaggcggct
     1741 cgaacgacgt cggtgcggac attaccgtgg tcgacccgaa gctcggcaaa cacacggtca
     1801 agtgggaccg catccgcacg atcgagttca gggagacgcc ggcgaagctc cccgccaagc
     1861 tgggcgagcc catctatggg accgtgaagt ccgggcggta cgacttcgtc ggtcgaatcc
     1921 agtgggacaa cgacgaggac ctgaccggcg acaaactcga cggcgaaacc caggacggca
     1981 aggtgtccgt cgcattcggc gacatcgcgg ccatccgtaa gcatcggcaa ggcgcgctgg
     2041 tgaggttgaa gtcgggctcc gagctgtacc tcaccggcac caacgacgtg aatcacggga
     2101 atcgcggcgt ggtcgtggtg gtggcgcgac tcggggccgt gaagatcggc tgggacgact
     2161 tcgacgaggt gacatttgcc cccgccccca actcgggacg cggctacgcc gagtacgcgc
     2221 aggcgagcga tctgagcggt gaggtcacga cccgtgacgg ccgctacgac ggccggatcg
     2281 ttttcgacct cgacgaatcc cgagacttcg agctgctcca gggcacgaac ggggacaccg
     2341 agtacctcat cccgttccgc gacattgcac ggatcacacc tcagggaagg tttcgctccg
     2401 aggtcgtgct gcacatgggg atgacgatcg agttggagga cagccaggac gtcacgcgca
     2461 ggaacgacgg cctgctggtt ttcaccgggg agcgcaaacc gaagtacgtc gcctggcagg
     2521 atgtcaccga cgtccggttt gaaccgcctc cccggtaggt cgcttttcta ggcgagcacg
     2581 gccgttccca actggctgac ctcgcggagc gtaatcggat cccagcgtcg tcgtttgccg
     2641 aatccgttcc gcgcccggcc gtcccaatcc cgcgctcgtc agcccggccg atccgccact
     2701 caaccacggc acccccttcc tgtgtcgaac cggcgtcgcc atcgactgac gcgcaccagg
     2761 agggatggtc atgcccagga cccggtggtt cgccctttcg
//
LOCUS       DASRIQ010000005         4680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_263158, whole genome shotgun sequence.
ACCESSION   DASRIQ010000005 DASRIQ010000000
VERSION     DASRIQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263158"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /locus_tag="VFD06_00070"
     CDS             complement(<1..1639)
                     /locus_tag="VFD06_00070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN01957.1"
                     /translation="MELAPNRVLGHYRLVEKIGEGGMGVVWRATDTTLGREVAIKILP
                     DLFAEDPDRLARFEREAKVLASLNHPGIATIHGLHHAEGMHFLAMELAPGIDLATRLA
                     AGPIPMDEAIGIALKVAEALEAAHDHGIVHRDLKPANIQIAPDGRIKILDFGLAKAFD
                     AEPGGTLSTSASLSPTLTTPAATRAGLILGTAAYMSPEQAKGKPVDRRADIWAFGCVL
                     YEMIGGKRPFQGDGVSELIAAVIMAPVDFETLPKSLPARVRLLVRRCMEKDPRRRLRD
                     IGEARIALEETLTGAPDPAAPAAVPAATASGARRRAAFAAAFGAAVAAALAAVGAWSL
                     RPAPTAVEVRRFEIPTQGAARSPQTVARAASISPDGKAVAYVEGGTLLVRRLAKLEPI
                     AIKTPAEPGAIFWSPDGAWLGYVAAGKLYKVPAGGGESSLIADMKTPLTGGSGASWCP
                     DGHIVLSTGDGPILRVSSLGGDLAPYVPLVAGQEGDLHDPECLPDNSVIFAPHLQGGR
                     PSVLVLFAGGQRKELLRLPPDQDIWFPVYSPTGHILYR"
     gene            complement(1699..3933)
                     /locus_tag="VFD06_00075"
     CDS             complement(1699..3933)
                     /locus_tag="VFD06_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN01958.1"
                     /translation="MEPTPDSTVSHYRIVRKLGRGGMGVVFEGEDTRLGRRVALKFLP
                     QDLAQDEPSLERFRREARAASALNHPNICTVYAIDQHAGQHFIVMELLEGETLAQRLA
                     GRPAEPAPMLDLAIQIADALESAHAKGIVHRDIKPANIFVNPRGQAKILDFGLAKIDV
                     ARRPAEGAGANSRMDTVVAGEELTQRGSTVGTVSYMSPEQARGQPTDARTDLFSLGTV
                     LYQMATGTLPFQGETSAVVFEAILNRDPAPVTQINSTLPPEFARILDKALEKDRTLRY
                     QTATDLKTDLIRLRRDLESGRRGAASASGEARAVTKAPEKTIAVLYFENLSGVKEDEY
                     LRDGITEDLITELSKIKGLNIFSRPTVLAYRDRQVTPTQIGQQLRASAVLTGSLRRAG
                     SRLRINAQLIDTHTDFPIWSERYDREMKDVFEVQDEIARKIAEALRITLSPQEQEALA
                     SRPTDDLQAYDLYLRGKSYARRLTRQDLEFALQMFENAVALDPKFALAHAAIAKVCAE
                     YHFNFQRDATWLERAMSSSARASALQPLLPEVQIAQAWVLYAGNQYDEAVRYAQQAIT
                     RKRDCEGAYYLLGRAMFSAGRFQEVVSMAEEAIDSAGEDYNVYVPILNSLGALGKNDQ
                     MRNLRLRRIQALEAHLKPVPEDARARVLLAGDYANIGRTEEATREADLAMALRPNEAT
                     VLYNLACLFCHLQKKQEALDALRKAWNAGFRESDWARRDPDLVLLHGDPEFERLYPAE
                     TPGA"
     gene            4018..4371
                     /locus_tag="VFD06_00080"
     CDS             4018..4371
                     /locus_tag="VFD06_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01959.1"
                     /translation="MMAFPGTLDGHFRQVEQLQAVRKSDLEMGDCLVVETRNSRYHIV
                     SLGDGTYRVSGGFFDRSGGPVVTTIAGCTWGGSAIHTDLAAAPGLFLEFGNRVMTTRI
                     RSLSVIPSRPSGSIH"
     gene            4383..>4680
                     /locus_tag="VFD06_00085"
     CDS             4383..>4680
                     /locus_tag="VFD06_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN01960.1"
                     /translation="MSLPAGSRIGPYEILGPLGAGGMGEVYRARDTRLGREVAIKILP
                     AAVAADHDALSRFEREARAVAALSHPNILAIHDFGVEGGRAYSVTELLDGRTLRE"
BASE COUNT          654 a         1576 c         1703 g          747 t
ORIGIN      
        1 ggcgatagag gatgtgcccg gtcggggagt agaccgggaa ccagatgtcc tgatcggggg
       61 ggaggcgaag caactccttg cgctggccgc cggcgaacag gacgagcacg ctcggccgcc
      121 cgccctggag atgcggcgcg aagatcaccg agttgtccgg caggcactcg gggtcgtgca
      181 ggtcgccttc ctgtccggcg acgagcggga cgtacggcgc gaggtcgcct ccgagcgacg
      241 agacgcgcag gatcgggccg tcgccggtgg acagcacgat atgaccgtcg gggcaccacg
      301 acgccccgga cccgccggtg agcggcgtct tcatgtcggc gatgaggctg ctttcgccgc
      361 cgccggccgg caccttgtac agcttcccgg ccgccacata gccgagccat gcgccgtcgg
      421 gggaccagaa gatggctccc ggctcggccg gtgtcttgat ggcgatcggc tcgagcttcg
      481 ccagacgtct cacgaggagc gtccccccct cgacgtacgc gaccgctttg ccatcggggg
      541 agatggaggc ggcgcgcgcc accgtctgcg gcgatcgcgc cgccccctgc gtcgggatct
      601 cgaagcggcg gacttctacg gccgttggcg caggtcgcaa cgaccatgca ccaacggccg
      661 cgagggcggc ggcgaccgcg gcgccaaagg cggcggcgaa agccgcgcga cggcgtgcgc
      721 cgcttgccgt cgccgccgga acggccgccg gcgcggccgg gtccggggcg ccggtgaggg
      781 tctcctcgag cgcgatgcgc gcctcgccga tgtcgcgcag gcgccggcgc ggatccttct
      841 ccatgcagcg gcgcacgagg aggcggacgc gcgccggcag cgacttcggc agggtctcga
      901 agtcgaccgg cgccatgatc accgccgcga tcagctcgga gacgccgtcc ccctggaacg
      961 ggcgtttccc gccgatcatc tcgtacagga cgcagccgaa cgcccagatg tcggcgcggc
     1021 gatcgaccgg cttccccttc gcctgctccg ggctcatgta ggcggcggtg ccgaggatga
     1081 gccccgcccg tgtcgctgcc ggggtggtca gcgtcggcga cagcgaggcg ctggtcgaca
     1141 gcgtgccccc gggctcggcg tcgaacgcct tcgccagccc gaagtcgagg atcttgatgc
     1201 ggccgtcggg ggcgatctgg atgttggccg gcttgaggtc gcggtgcacg atgccgtggt
     1261 cgtgcgccgc ctcgagcgct tcggccacct tgagggcgat gccgatcgcc tcgtccatcg
     1321 gaatcgggcc cgcggcgaga cgcgtcgcga ggtcgatccc cggcgccaac tccatcgcca
     1381 ggaagtgcat gccctcggcg tgatgcagcc cgtggatggt ggcgatgccg ggatggttga
     1441 gggaagcgag caccttcgcc tcgcgctcga agcgggcgag gcggtcggga tcctcggcga
     1501 acagatccgg gagaatcttg atcgcgactt cgcgcccgag ggtcgtgtcg gtggcgcgcc
     1561 acacgacgcc catcccccct tcgccgatct tctcgacgag gcggtagtgc ccgaggacgc
     1621 ggttcggtgc gagctccatt caggcggccg gcaccgagga tcgcgtgcgc gtttcccgcg
     1681 ggacgaccgt cggcgggctc acgcgccggg cgtctcggcg gggtagagcc gctcgaactc
     1741 cggatcgccg tgcagcagca ccaggtccgg gtcgcgccgg gcccagtccg actcccggaa
     1801 accggcgttc caggccttgc gcagcgcgtc gagcgcctcc tgcttcttct gcagatgaca
     1861 gaagaggcag gcgaggttgt agaggacggt cgcctcattc ggccgcagcg ccatggcgag
     1921 gtcggcctcg cgcgtcgcct cctcggtccg accgatgttg gcgtagtccc cggccaggag
     1981 cacgcgggcc cgagcgtcct ccggtaccgg cttcagatgc gcttcgagcg cctggatgcg
     2041 gcgcagtctg aggttccgca tctggtcgtt cttgccgagg gcgccgaggg aattgaggat
     2101 cggcacgtag acgttgtagt cctcgccggc cgagtcgatc gcctcctcgg ccatgctgac
     2161 cacctcctgg aagcggccgg ccgagaacat ggcgcggccg agcaggtagt aggctccttc
     2221 gcagtcgcgc ttgcgcgtga tcgcctgctg ggcgtaacgc accgcctcgt cgtactggtt
     2281 gccggcgtac agcacccagg cctgggcgat ctgcacctcc ggcagcagcg gctgcagcgc
     2341 cgaggcgcgc gccgacgacg acatggcccg ctcgagccag gtggcgtcgc gctggaagtt
     2401 gaaatggtat tcggcgcaga ccttggcgat ggcggcgtgg gccagggcga acttagggtc
     2461 gagcgcgacc gcgttctcga acatctgcag ggcgaactcg agatcctggc gcgtcagccg
     2521 gcgcgcgtac gacttgccgc gcaggtacag gtcgtacgcc tgcaggtcgt cggtcggccg
     2581 cgacgccagc gcctcctgtt cctgggggga cagggtgatg cgcagcgcct cggcgatctt
     2641 ccgggcgatc tcgtcctgca cctcgaagac gtccttcatc tcccggtcgt agcgctccga
     2701 ccagatcggg aaatcggtgt gcgtgtcgat gagctgcgcg ttgatgcgca ggcggctgcc
     2761 ggcgcgccgc agcgagccgg tgagcaccgc cgaggcgcgc agctgctggc cgatctgcgt
     2821 cggggtgacc tgcctgtcgc ggtacgccag gacggtggga cgcgagaaga tattcagccc
     2881 cttgatcttc gagagctcgg tgatcaggtc ctcggtgatc ccgtcgcgca ggtattcgtc
     2941 ctccttcacg ccgctcaggt tctcgaagta gaggactgcg atcgtcttct cgggagcctt
     3001 ggtgacggcg cgcgcctccc ccgaggcgga ggccgcgccg cggcgccccg actcgaggtc
     3061 gcgccgcagg cggatcagat cggtcttcag gtcggtcgcc gtctggtaac ggagcgtgcg
     3121 gtccttctcg agcgccttgt cgaggatgcg ggcgaactcg ggtggcagcg tgctgttgat
     3181 ctgcgtcacc ggagccggat cgcggttcag gatcgcctcg aacacgacgg cggaggtctc
     3241 cccctggaac ggcagcgtcc cggtcgccat ctggtacagc acggtgccga gcgagaacag
     3301 gtcggtgcgc gcgtcggtcg gctggccgcg cgcctgctcc ggcgacatgt aggagacggt
     3361 gcccacggtc gagccgcgct gcgtcagctc ctctcccgcg accaccgtgt ccatgcggga
     3421 gttggccccg gcgccttcgg ccggtcggcg ggcgacgtcg atcttcgcga gaccgaagtc
     3481 gaggatcttc gcctggccgc gcgggttgac gaagatgttg gccggcttga tgtcgcggtg
     3541 gacgatcccc ttggcgtgcg ccgactcgag cgcgtcggcg atctggatcg ccaggtcgag
     3601 catcggcgcc ggctcggccg ggcggccggc gagccgctgc gccagcgtct ccccctcgag
     3661 cagctccatg acgatgaaat gctggccggc gtgctggtcg atggcataga ccgtgcaaat
     3721 gttgggatgg ttcagcgccg aggcggcgcg cgcctcgcgc ctgaagcgct cgagagaggg
     3781 ttcatcctga gccaggtcct gaggcaggaa cttgagcgcc acgcgtcgtc ccaggcgggt
     3841 gtcctcccct tcgaacacca cccccatgcc gccccggccg agtttgcgga cgatgcggta
     3901 atgcgagacg gtgctgtcgg gggtgggttc catggggcgg aggcggatgt tacccggagc
     3961 gaatatgcga tgcaagcaag ccgttcgggg ggatgggtcg attagaatga ggtggacatg
     4021 atggcgttcc ccggcacgct ggacggccat ttcaggcagg tcgagcagct tcaggccgtc
     4081 cgcaagagcg atctcgagat gggcgactgt ctcgtcgtcg aaacacgcaa ttcccggtat
     4141 cacatcgtca gcctggggga cgggacctat cgggtgtcgg ggggcttctt cgatcgctcc
     4201 ggcggcccgg tggtgaccac gatcgcgggc tgcacctggg ggggcagcgc catccacacc
     4261 gatctcgccg ccgccccggg cctcttcctc gaattcggca accgcgtcat gaccacccgc
     4321 atccggtcct tgagcgtgat cccctcccgt ccctccggct cgattcactg atccgcttcc
     4381 cggtgtccct ccccgccggc tcgcgcatcg gcccctacga gatcctcggc cccctcggcg
     4441 ccggcggcat gggggaggtg taccgggcgc gcgacacgcg gctgggacgc gaggtggcga
     4501 tcaagatcct gcccgcggcg gtggcggcgg atcacgacgc actgtcccgg ttcgagcgcg
     4561 aggcgcgcgc cgtcgcggcg ttgtcgcacc cgaacatcct ggccatccac gacttcggcg
     4621 tcgagggcgg gcgggcgtac tcggtcaccg agctgctgga tggacgcacg ctgcgcgagc
//
LOCUS       DASRIQ010000006         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1045749, whole genome shotgun sequence.
ACCESSION   DASRIQ010000006 DASRIQ010000000
VERSION     DASRIQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045749"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VFD06_00090"
     CDS             <1..1657
                     /locus_tag="VFD06_00090"
                     /inference="COORDINATES: protein motif:HMM:NF037833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01961.1"
                     /translation="DPAPAGDACRIACDAAAFPITRLPPWQRMIRRGDTIYTYLAEDV
                     DANGSPDSWCLVGSDSAPNMPDELLVGMALTSHAGAQTGTITYDNMTIEPLDDCGDSV
                     CTIGEELAAFDFTAPDGTNPALGGLTVVQGGAFAPVIMGNRLRLSQEGIGSIANAVWG
                     PPADLGDTGFVAEFDAYMSHSVLPGDANPADGMTFAVVQGDAALAVGLRGDGGGALGY
                     EGFTLRERTECHPSFSVELDNWVGGGEPSNEPGTGGSPNNDGRYHVGLNVNASVGSIQ
                     TNEQFGVPVTSLPNIFDADGVHVEVQYSASGHINVYVSDGTVPRTQVISACIDPLAGD
                     LLIGFTAGTGGATATQEVDNVVLSSVCCEDEDTVSITGATEGLVGTPVVLTANAGGID
                     GAATYTWSIESGPGTITPSGNTASVDATGADDVVVKVVFTDGTCGGSAEDTHTISFDT
                     PGGLQKPNDMNGDGSLDISDPVATLNHLFLGGPGPACGDNTITHPSNLTLLDANDSGA
                     IDISDPVFVLNFLFLGGPTPGNCNGDVSCPCIVIPECPDVCTP"
BASE COUNT          362 a          771 c          677 g          334 t
ORIGIN      
        1 cgatccggcg cctgcgggcg atgcctgccg cattgcctgc gatgccgcgg ccttcccgat
       61 cacccgtctg ccgccgtggc agaggatgat ccgccgcggg gacacgatct acacctacct
      121 cgccgaggat gtcgacgcca atggcagccc cgacagctgg tgcctggtcg gctccgatag
      181 cgcgccgaac atgcccgatg agctgctcgt cggtatggcg ctcacgtccc atgccggcgc
      241 ccagaccgga acgatcacgt acgacaacat gaccatcgag cctctcgacg actgcggcga
      301 cagcgtctgc acgatcggcg aggagctcgc ggccttcgac ttcaccgctc cggacgggac
      361 caacccggcc ctcggcggcc tcaccgtcgt ccagggcgga gcctttgcgc ccgtgatcat
      421 gggcaaccgc ctgcggctct cgcaggaggg catcggcagc atcgcgaacg cggtctgggg
      481 tcctccggct gatctgggcg acaccggctt cgtggccgag ttcgatgcgt acatgagcca
      541 ctcggtcctc cccggcgacg ccaacccggc cgacggcatg accttcgccg tcgtccaggg
      601 tgacgcggcg ctggctgtcg gccttcgcgg cgacggcggc ggcgctctcg gctacgaggg
      661 cttcacgctc cgcgagcgca ccgagtgcca cccgagcttc tcggtcgagc tcgacaactg
      721 ggtcggcggc ggcgagccgt cgaacgagcc cggaacgggc ggctctccca ataacgatgg
      781 caggtaccac gtcggcctca acgtcaacgc gagcgtcggc tcgatccaga cgaacgagca
      841 gttcggcgtc ccggtcacga gcctgccgaa catcttcgac gccgacggcg tccatgtcga
      901 agtccagtac tccgcttccg gccacatcaa cgtgtacgtc tccgacggca cggtcccgcg
      961 cacgcaggtg atctcggctt gcatcgatcc gctcgccggc gatctgctca tcggcttcac
     1021 ggcgggcacg ggcggcgcca cggcgaccca ggaggtcgac aacgtggtgc tcagctcggt
     1081 ctgctgcgag gacgaggaca ccgtgtcgat caccggcgcc accgagggtc tggtcggcac
     1141 tcccgtggtc ctgacggcga acgccggcgg gatcgacgga gcggccacct acacgtggtc
     1201 catcgagagc ggccccggca ccatcacgcc gagcggcaac acggcttcgg tcgatgccac
     1261 cggcgcggat gatgtcgtcg tcaaggtcgt cttcacagac ggcacctgcg gcggctccgc
     1321 ggaggacacg cacacgatct ccttcgatac cccaggcggc cttcagaagc cgaacgacat
     1381 gaacggcgat ggcagcctcg acatctcgga cccggtcgca acgctgaacc acctgttcct
     1441 tgggggccca ggcccggctt gcggtgacaa cacgatcacc cacccgtcga acctgacgct
     1501 tctcgacgcg aacgacagcg gagcgatcga catctccgac ccggtgttcg tgctcaactt
     1561 cctcttcctg ggcgggccca cgccgggcaa ctgcaacggc gacgtgagct gcccgtgcat
     1621 cgtgattccg gaatgcccgg acgtgtgcac gccgtaatct agtcacgccc tgtgcgtgaa
     1681 gcgggcccgg ttccggaagg agccgggccc gctttctttt tggcccaatc tccaggctcg
     1741 cgaagcttga ccgttctcgc gctcaccgcc acactttcgg catgagaacg tcgatgcgat
     1801 cgattgtcct ggcggcgagt ctcctctcca cggccgaact ggcgtgcgag agaaaggaag
     1861 atccttcctc caccgccccg acaccgcccc ggaaggatcc ggaacctccc gcgccgctct
     1921 tacccatctt tcccgagaag gcgccctggg agccggagcc ggacctcgtc cctgctcccc
     1981 aggaaacggc tccgaccccc ccaaaggccg aggatccgcc gaggaagctg gtgccaccgg
     2041 actggtggga ggaccgcgag aacttctacg tgaacgccaa ggatctcatc cggcagggtc
     2101 ggaagaagga ggcgctcgaa gtcctcgaag acgtgctcgc cctg
//
LOCUS       DASRIQ010000007         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1046246, whole genome shotgun sequence.
ACCESSION   DASRIQ010000007 DASRIQ010000000
VERSION     DASRIQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046246"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFD06_00095"
     CDS             <1..556
                     /locus_tag="VFD06_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZN01962.1"
                     /translation="NVNAAIGAGKNSQDYVARTLTYALEQREKSLPNALIRAGGLEMV
                     RFNRGISILDTCITVAPLLGLLGTVTGMMGSFGMLGGGELSAPAQITGGIAEALIATC
                     FGLGIAVTALLPMNYLHSQSDKARHEMEDASNHLEILMKPVLEAEQAVRDRRIRKLMQ
                     QQDERDGLDIEPAAGGSTRAAATV"
     gene            572..979
                     /locus_tag="VFD06_00100"
     CDS             572..979
                     /locus_tag="VFD06_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZN01963.1"
                     /translation="MKLTAYQHKKAKIEIVPLIDVVFFLLATFVMVSLSMTRNQGMQV
                     ALPAASSAAKQADDDKAVTLTVMATGEVFWNKEKITAAQLPFRLQTLKTAQKEPKVIL
                     NAAADADFKQVVAVLDETRKIGITKVGISTEKK"
     gene            976..1707
                     /locus_tag="VFD06_00105"
     CDS             976..1707
                     /locus_tag="VFD06_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZN01964.1"
                     /translation="MKKVILLAFAVAIVFHVAILGFGGLLFNHGKAEKVVREDVDLVG
                     DEEKKDDKKDEPKPVEKAEAKEADEEAIEQETPVAPDLRDLQALEAPSAPALAAMSLS
                     DLESALGGGMGGGGGDFGGAGGPGLASGGRIGGTGSADGGGSSLDDMLTAAELDQRPR
                     PIFQATPQYPIELRKQKIEGTVNVVFYVDKDGKVSNPQVETSTHPAFERPALEAVRQW
                     RFDPGTRKGEKVSFKMRIPISFHVS"
     gene            1697..3136
                     /locus_tag="VFD06_00110"
     CDS             1697..3136
                     /locus_tag="VFD06_00110"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN01965.1"
                     /translation="MSRKPLHLALLLLLTFGTAIAAPKETESVKPAAAAEKPPADDPT
                     GLSLWKDTTFQKQFMGSYGALADYEPKLSPVERTELEKVLALMAVNQDSAISQLQAFI
                     TPESSALFDFILGNLYFQKDNSEEAVKHYRIATNKFPSFRRAFKNLGLTSVRLQRYDD
                     AIKALSRLVELGGADGLSFGLLGYSLSATGQFVSAESAYRNAVLLQPDALDWKLGLAQ
                     SVLKQRKFGEVVSLCDELLAKYPDRADFWLLQANAWLGQDQPMKAAENFEIVARIGKA
                     TFQTWYTLGDIYTNAGLYDLAANAYKQAIVAKPDQELDRPLRSVEILAQRDAVPQARE
                     LLAQVRESSGGILDDAGKKRVLRLDARLAMAEGGAAGAVDGSGEAAAVKSLEEIVALD
                     PLDGEALMLLGQHYTKAKEIDRAIFYYERASSLEPFEADAKVRQAQLLVTQNKYREAV
                     PLLKRAQELKPRDEVARYLDQVERISRSH"
     gene            3309..>3950
                     /locus_tag="VFD06_00115"
     CDS             3309..>3950
                     /locus_tag="VFD06_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01966.1"
                     /translation="MRAKRNVWSGFPGLAIVLGAGLAGSAGPAAAAEPTPTLEDVLAY
                     LHFDDAERARVLQGEIVGKDFREHDEKEISVAVVTRIATPLPKLAEEIRKGELLHADQ
                     EVLDFRASAPGTPLEETFRTAGYTSEEGEEVRRLLAALPGSTFNLSAGELDRLRAART
                     RFPGGDCDRKPACAGELSGLYRGMLQERLARYQADGLNGVAPYDRGSGKKVLPA"
BASE COUNT          691 a         1335 c         1363 g          561 t
ORIGIN      
        1 gaacgtgaac gccgccatcg gggccggcaa gaactcgcag gactatgtcg cccgcaccct
       61 gacctacgcc ctcgagcagc gggagaagtc gctgcccaac gccctcatcc gggcgggcgg
      121 cctggagatg gtccgcttca accgcggcat ctcgatcctc gacacctgca tcacggtggc
      181 gccgctcctc gggctcctcg ggacggtcac cgggatgatg gggtcgttcg gcatgctggg
      241 aggtggcgag ttgagcgccc cggcgcagat caccggaggg atcgccgagg cgctcatcgc
      301 cacctgtttc ggtctcggca tcgcggtcac ggcgcttttg ccgatgaact acctgcacag
      361 ccagagcgac aaggcgcgcc acgagatgga ggacgcctcg aaccacctcg agatcctgat
      421 gaagccggtg ctcgaggcgg agcaggcggt gcgcgatcgc cgcatccgga agctgatgca
      481 gcagcaggat gagcgcgacg ggctcgacat cgagccggcg gcaggcggtt cgacgcgcgc
      541 cgcggccacg gtctgacgcg ggggtgagcc gatgaaactg accgcctacc agcacaagaa
      601 ggcgaagatc gagatcgtcc cgctcatcga cgtcgtcttc ttcctgctcg cgaccttcgt
      661 catggtgtcg ctgtcgatga cgcgcaacca ggggatgcag gtggcgctgc cggcggcgtc
      721 gagcgccgcc aagcaggcgg acgacgacaa ggcggtcacc ctgaccgtga tggcgaccgg
      781 cgaggtcttc tggaacaagg agaagatcac cgcggcgcag ctgccgttcc ggctgcagac
      841 cctcaagacg gcgcagaagg agcccaaggt catcctgaac gccgcggccg acgccgactt
      901 caagcaggtg gtggcggtcc tcgacgagac ccgcaagatc ggcatcacca aggtcggcat
      961 ctcgacggag aagaaatgaa gaaggtcatc cttctggcgt tcgccgtcgc gatcgtcttc
     1021 cacgtcgcca tcctcggctt cggcgggttg ctgttcaacc acggcaaggc cgagaaggtg
     1081 gtgcgcgagg acgtcgacct cgtcggcgac gaggagaaga aggacgacaa gaaggacgag
     1141 ccgaagccgg tggagaaggc cgaggcgaag gaggccgacg aggaggcgat cgagcaggag
     1201 acgccggtcg ccccggatct ccgcgacctg caggctctcg aggcaccgtc ggcgcctgcg
     1261 ctcgccgcca tgagcctgag cgacctcgag tcggcgctcg gcggcggcat gggtggaggc
     1321 ggtggcgatt tcggcggcgc gggcggcccg gggctggctt ccggcggccg catcggcggc
     1381 accggctcgg ccgacggtgg cggcagcagc ctcgacgata tgctcacggc ggcagagctc
     1441 gaccagcgcc cgcggcccat cttccaggcg acgccgcagt acccgatcga gctgcgcaag
     1501 cagaagatcg aagggaccgt caacgtcgtc ttctacgtgg acaaggacgg gaaggtcagc
     1561 aacccccagg tcgagacctc gacccacccg gcgttcgagc gccccgccct cgaagcggtg
     1621 cgccagtggc gcttcgaccc ggggacgcgc aagggggaga aggtctcctt caagatgcgc
     1681 atcccgatct cgttccatgt ctcgtaagcc gcttcacctc gcgctcctgc ttctcctgac
     1741 cttcggcacg gcgatcgcag cgccaaaaga gacggagagc gtcaagcccg cggcggcggc
     1801 ggagaagccg ccggccgacg atcccaccgg tctctccctc tggaaggaca cgaccttcca
     1861 gaaacagttc atggggtcgt acggggcgct ggcggattac gagccgaagc tgtctccggt
     1921 cgagcggacc gagctggaga aggtgctcgc cctgatggcg gtcaaccagg actccgccat
     1981 ctcgcagctg caggcgttca tcacgccgga gtcgagcgcg ctgttcgact tcatcctcgg
     2041 gaacctctac ttccagaagg acaactccga ggaggcggtc aagcactaca ggatcgccac
     2101 gaacaagttc ccgtcgttcc ggcgcgcctt caagaacctc ggcctgacgt cggtgcgcct
     2161 gcagcgctac gacgacgcca tcaaggcgct gagccgcctg gtggagctgg ggggcgcgga
     2221 cggcctgtcg ttcgggctcc tgggctattc gctgagcgcc accggccagt tcgtctcggc
     2281 cgagtcggcg taccgcaacg ccgtcctgct gcagccggac gccctcgact ggaaactcgg
     2341 gctggcgcag agcgtcctga agcagcgcaa gttcggcgag gtggtcagcc tgtgcgacga
     2401 actgctggcg aagtatcccg accgcgccga cttctggctc ctgcaggcga acgcctggct
     2461 cggccaggac cagccgatga aggcggccga gaacttcgag atcgtcgccc gcatagggaa
     2521 ggcgaccttc cagacctggt acaccctcgg cgacatctac accaacgccg gcctctacga
     2581 cctcgccgcc aacgcctaca agcaggcgat cgtcgccaag ccggaccagg agctggaccg
     2641 ccccctgcgc tccgtcgaga tcctcgccca gcgcgacgcc gtcccgcagg cccgcgagct
     2701 gctggcgcag gtgcgcgagt cgtccggcgg gatcctcgac gacgccggga agaagcgcgt
     2761 gctgcggctc gatgcgcgtc tcgccatggc cgagggcggc gccgccggag cggtcgacgg
     2821 cagcggtgag gcggccgcgg tcaagtcatt ggaggagatc gtcgctctcg acccgctaga
     2881 cggcgaagcg ctgatgctcc tgggtcagca ctacaccaag gcgaaggaga tcgaccgggc
     2941 gattttctac tacgagcgcg ccagcagcct cgagcccttc gaggccgacg ccaaggtccg
     3001 ccaggcccag ctcctcgtca cccagaacaa gtaccgcgaa gccgtccccc tcctcaagcg
     3061 cgcccaggaa ctcaagcccc gcgacgaggt cgcccgctac ctcgaccagg tcgagagaat
     3121 ttcccgcagt cactgatccg agtctcaggg cttgcgccag agccggcgtg gaccaaagcg
     3181 acggatcctg ggcggcgcag tcggccgcat cgggtgtccc gtcgccgtcc atgtcgcccg
     3241 gtgacggcgc gagaggtcgc ccggagcagg acggcggtgc gcgatcctca tctacacttg
     3301 tcggcatcgt gcgagcgaag agaaacgtat ggagcggctt cccgggcctc gccatcgtcc
     3361 tgggcgcggg tctcgcgggg tccgccggcc cggccgcggc ggcggagccg actcccacgc
     3421 tcgaggacgt cctggcgtac ctgcatttcg acgatgcgga gcgcgcccgg gtcctccaag
     3481 gggagatcgt cggcaaggac ttccgtgagc acgacgagaa ggagatctcc gtcgcggtcg
     3541 tgacccggat cgcgacgcca ctgccgaagc ttgccgaaga gatccggaag ggggagctgc
     3601 tccatgccga tcaggaggtc ctcgacttcc gtgcgtcggc gccgggaact ccgctcgagg
     3661 agaccttccg gacggccggc tacacctcgg aggagggtga ggaggtccgc cggctcctgg
     3721 cggccctgcc gggctccacg ttcaacctca gcgcagggga gctggatcgg ctgcgcgccg
     3781 cgcggactcg tttccccggc ggcgattgcg accggaagcc agcctgcgcc ggggagcttt
     3841 cgggcctcta tcgcgggatg ctgcaggagc ggctggcgcg ctaccaggca gacggactga
     3901 acggggtcgc cccctacgac cgtggctcgg ggaagaaggt cctccccgcg
//
LOCUS       DASRIQ010000008         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_4055, whole genome shotgun sequence.
ACCESSION   DASRIQ010000008 DASRIQ010000000
VERSION     DASRIQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4055"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFD06_00120"
     CDS             <1..996
                     /locus_tag="VFD06_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZN01967.1"
                     /translation="ARLSDLGTRLVEASAAVPRDVGTAMNTLLGQMGARLAENAQAVQ
                     KIGSDTGRLISDLNVRLGELGKSSQQILELGQDVRGLQQIFQAPKTRGALGEVSLNAI
                     LEQCFPAEHVRIQHEFKSGDRVDALLRLPGGSVSIDSKFPLSAFRKRLEAQGDDERTR
                     AGRAFGREVRKHVDDIASKYIRPVEGTLDFALMYIPAESVFYDVVVRAEDSEEDINEY
                     ALKQRVIMVSPNSLYAYLQAIGFGLMGLRIEERAREILVGLQQVGADLGQFREGFELG
                     QRHLRNSQAAFGEAAERLARLQALIDQFSRSVAIAETLPPRAVSPRLRDAGPLFQ"
     gene            993..2465
                     /gene="truA"
                     /locus_tag="VFD06_00125"
     CDS             993..2465
                     /gene="truA"
                     /locus_tag="VFD06_00125"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZN01968.1"
                     /translation="MSDQRGGGGGGRRPPGPPRGGRPGPPRGDRPRPPHGGGPPRPWK
                     DRPPRPAGEGGAPRPWKDRPPRPAGEGGGRPWKDRPRPQGGGGSRPWKDRPPRPAGEG
                     GAPRPWKDRPPRPAGEGGGRPWKDRPRPQGGGGSRPWKDRPPRPAGEGGAPRPWKDRP
                     PRPAGEGGPPRPWKDRPPRPHGGGAPRDRGRPEGDRDRRDRPSPPERAEPAAGFRAFR
                     PPAPTGPERNIRLILEYEGTRYAGWQVQKTARTVAGEILAVIERTLGEQPQLLAAGRT
                     DQGVHAEMQVANFLTRSVMPPKALADLLNDALPADINVLRADEVPRSFHSRHDAIARR
                     YRYQIAERRTAFFKKLVWWIRQPLDLAAMEQAARDLVGRHDFSSYSDRTEELREPRVR
                     VHTAALRRSDFLLTFTIEADHYLPRMVRRIVGVLVQIGQGRLPVEAVQRFLKEQVNDP
                     AQWTAPSSGLLLEKVMYPSSRPTAAKTPAAPTPPRKEGSR"
     gene            2462..>3820
                     /locus_tag="VFD06_00130"
     CDS             2462..>3820
                     /locus_tag="VFD06_00130"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZN01969.1"
                     /translation="MTDRPARRLRSAPGLAAGLFGVAFAAGPAGPLPVEIGPTLERVD
                     VDRMRADLKFLSDDLFEGRGTGQRGGELAARYLETRYRLIGLKPGASETSWLQEVPLI
                     GVEALPDSRFEIEAGGKRFVPERLVDVVAASETQEESTSIDAPVVFVGYGITAPQHQW
                     DDFKGIDVRGKVLLMLVNDPPSDDPAFFGGPALTYYGRWTYKYESAARAGARGAILIH
                     TDASAGYGWNVVKNSWGRERPYVPLPKDGPKPLGLVSWVTERMARQVLAAAGEDFETL
                     RSAAARRDFRPRPLTMKVRVQIHQKVRPIATWNVLGLLPGSDPKLKEEAVVVMAHYDH
                     LGIGPAENGDAIYNGAADNASGVSALLETARLFTSLPRPPRRTLLFLAVGAEEGGLRG
                     SEYYVRHPVVPLERTAAALNMDGLPVDGAPRDYTFLGSDRTTLREVVRDASAVLDFEV
                     IPD"
BASE COUNT          551 a         1386 c         1368 g          515 t
ORIGIN      
        1 gcacggctgt cggacctggg gacccgcctc gtcgaggcga gcgcggcggt gccgcgcgac
       61 gtcgggacgg cgatgaacac gctgctcggt cagatggggg cgcgccttgc cgagaacgcc
      121 caggcggtgc agaagatcgg cagcgacacc ggccggctca tttccgacct gaacgtgcgg
      181 ctcggcgagc tgggaaagag cagccagcag atcctcgagc tgggacagga cgtccgcggc
      241 ctgcagcaga tcttccaggc ccccaagacc cgcggcgcgc tcggcgaagt gtcgctcaac
      301 gcgatcctcg agcagtgctt ccccgccgag cacgtgcgca tccagcacga gttcaagagc
      361 ggcgaccggg tcgacgccct gctgcggctg cctggagggt cggtgtcgat cgactcgaag
      421 ttcccgctct ccgccttccg caagcggctc gaggcgcagg gcgacgacga gcggacgcgc
      481 gccgggcgcg ccttcggacg cgaggtgcgc aagcacgtcg atgacatcgc ctccaagtac
      541 atccggccgg tcgaggggac cctcgacttc gcgctgatgt acatcccggc ggagagcgtc
      601 ttctacgacg tggtggtgcg cgccgaggac agcgaggagg acatcaacga gtacgccctg
      661 aagcagcggg tgatcatggt gtcgccgaac tcgctctacg cctacctgca ggccatcgga
      721 ttcggtctca tggggctgcg catcgaggaa cgggcgcggg agatcctcgt cgggctgcag
      781 caggtcggcg ccgacctcgg ccagttccgc gaagggttcg agctcggcca gcgccacctg
      841 cgcaactcgc aggcggcgtt cggcgaggcg gccgagcggc tggcgcggct ccaggcgctc
      901 atcgatcagt tctcgcgctc ggtggcgatc gccgaaacgc tgccgccgcg cgcggtgtcg
      961 ccgcggctcc gcgacgccgg tcccctcttc cagtgagcga ccagcgcggc ggcggcggtg
     1021 gcggcaggcg tccgcccggc cctccgcggg gcggccggcc cggcccgccg cgcggcgatc
     1081 gaccgcgtcc ccctcacggg ggcggtccgc cccggccgtg gaaggatcga cccccgcgac
     1141 ctgcaggaga aggcggcgct ccgcgtccgt ggaaggaccg gccgccccgt ccggcaggag
     1201 aaggaggcgg tcgcccttgg aaggatcgtc ctcgcccgca ggggggcggc ggatcgcgtc
     1261 cctggaagga tcgtcccccg cgacctgcag gagaaggcgg cgctccgcgt ccatggaagg
     1321 accggccgcc ccgtccggcg ggagaaggag gcggtcgccc ctggaaggat cgtccgcgcc
     1381 cgcagggggg cggtggttcg cgcccctgga aggaccgtcc cccgcgtccg gcaggagaag
     1441 ggggcgctcc gcgtccgtgg aaggatcgac caccccgtcc ggcgggtgaa gggggtcctc
     1501 cgcgaccctg gaaagaccgg ccgccccggc cgcacggggg cggcgcgccg cgcgatcgcg
     1561 gccggcccga gggagaccgc gatcggcggg atcggccctc cccgcccgag agggctgagc
     1621 cagccgcggg ctttcgtgcc ttccgaccgc ccgcaccgac cggcccggag cgcaacatcc
     1681 ggctcatcct cgaatacgaa gggacgcggt acgccggctg gcaggtgcag aagaccgccc
     1741 gcaccgtcgc cggcgagatc ctcgccgtca tcgaacgcac tctcggcgag cagccgcagc
     1801 tgctcgcagc cggccgcacc gaccaggggg tgcacgccga gatgcaggtg gcgaacttcc
     1861 tgacccgatc ggtcatgcct ccgaaggcgc tcgccgatct cctcaacgac gcgctgccgg
     1921 ccgacatcaa cgtgctgcgg gcggacgagg tgccgcggag cttccactcc cgccacgacg
     1981 cgatcgcccg gcgctaccgc tatcagatcg ccgagcggcg caccgcgttc ttcaagaagc
     2041 tggtctggtg gatccgccag ccgctcgatc tcgcggcgat ggagcaggcg gcgcgcgatc
     2101 tcgtcggtcg acacgatttc tcctcctaca gcgaccgcac cgaggagctg cgcgagccgc
     2161 gcgtgcgggt gcacacggcg gcgctgcgcc gcagcgactt tctgctcacc ttcaccatcg
     2221 aagcggatca ctacctgccg cgcatggtgc ggcggatcgt cggggtgctg gtgcagatcg
     2281 gccaggggcg gcttccggtg gaagcggtcc agcgcttcct caaggagcag gtcaacgacc
     2341 cggcgcagtg gaccgccccg tcctcgggac tcctgctcga gaaggtgatg tacccctcgt
     2401 cacgcccgac cgcggcgaaa accccggcgg cgccgacacc gccgcggaag gaaggatccc
     2461 gatgaccgat cgtcccgcgc gtcgtctccg ctccgcgccc gggctcgccg ccggcctgtt
     2521 cggcgtcgcg ttcgccgccg ggccggccgg ccccctgccg gtcgagatcg ggccgacgct
     2581 cgagcgggtc gacgtggacc ggatgcgcgc cgacctcaag ttcctctccg acgatctctt
     2641 cgaagggcgc ggaaccggtc agcgcggcgg cgagctggcc gcgcgctacc tcgagacgcg
     2701 ctaccggctc atcggcctga agccgggcgc ctcggagacc tcctggctgc aggaggtccc
     2761 actcatcggc gtcgaggcgc tccccgacag ccgcttcgag atcgaggcgg gcgggaagcg
     2821 cttcgtcccc gagcggctgg tcgacgtcgt ggccgcgagc gagacccagg aggagtcgac
     2881 ctccatcgac gccccggtcg tgttcgtcgg ctacggcatc acggcgccgc agcaccagtg
     2941 ggacgacttc aaggggatcg acgtccgcgg caaggtgctc ctgatgctgg tcaacgaccc
     3001 gccgtcggac gatccggcgt tcttcggcgg tccggccctt acctactacg gccgctggac
     3061 ctacaagtac gagagcgcgg cgcgcgccgg ggcgcgcggc gccatcctga tccacaccga
     3121 cgcctcggcc ggctacggct ggaacgtcgt caagaactcg tggggtcgcg agcggccgta
     3181 cgttccgctg ccgaaggacg gccccaaacc tctcggtctc gtctcctggg tgacggagcg
     3241 catggcccgg caggtgctgg ccgcggccgg cgaggatttc gagacgctgc gatccgcggc
     3301 ggcgcggcgc gacttccgtc cgcgcccctt gacgatgaag gtccgggtgc agatccatca
     3361 gaaggtccgg ccgatcgcga cctggaacgt cctcggcctc ctcccgggtt cggacccgaa
     3421 gctcaaggag gaagcggtcg tcgtcatggc ccactacgat cacctgggga tcggccccgc
     3481 cgagaacggc gacgccatct acaacggcgc cgccgacaac gcctccggtg tttcggccct
     3541 gctcgagacg gcgcggctgt tcacttcgct gccgcgcccg ccgcgtcgca ccctgctctt
     3601 cctcgccgtc ggcgccgagg agggggggct gcgcggctcg gagtactacg tgcgccaccc
     3661 ggtcgtgccg ctcgagcgca ccgccgccgc cctcaacatg gacgggctgc cggtcgacgg
     3721 ggcaccgcgc gactacacct tcctggggtc cgatcggacg acgctgcgcg aggtggtgcg
     3781 cgacgcgtcg gcggtcctcg acttcgaggt gattcccgat
//
LOCUS       DASRIQ010000009         4309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1307640, whole genome shotgun sequence.
ACCESSION   DASRIQ010000009 DASRIQ010000000
VERSION     DASRIQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307640"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..930)
                     /locus_tag="VFD06_00135"
     CDS             complement(250..930)
                     /locus_tag="VFD06_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HZN01970.1"
                     /translation="MTLSHRAYVLVLLGLFALWWLALAIAPVDRADWMLENALVVVAL
                     AVLVVTDRWFRFSRVSYTLIFLFLCLHEIGAHYTYAKVPYHDWLQGIGVSLGAGRNHF
                     DRLGHFAFGLLLAYPMREVFVRVASARGFWGYYLPLDMTMAFSMLFELIEWAAAEIFG
                     GDLGVAYLGTQGDPWDAQKDMVLASLGGLIAMVVTALVNMRQQRDFAREWVESLRVKR
                     EGRRRASP"
     gene            complement(959..1969)
                     /locus_tag="VFD06_00140"
     CDS             complement(959..1969)
                     /locus_tag="VFD06_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZN01971.1"
                     /translation="MDPLTHGLLGAAVAQALLPASEGRKALLLGAAAGTLADADVLLR
                     LVSDPALPWEVHRHATHALIASPLGGLVAALPFLLGAGGRAQWKRTLLAATLAYATHG
                     LLDACTSYGTSLLLPFSGVRAAWDVIAIVDPLFTLPLLVGALLAAWRLRRAPALAALM
                     LCAAYLGLGARQHATALQEVHRLAASRGHVVERARALPTPMNLVVWRGLYVSEGRIHV
                     DGGRVPFASAPRFRPGTSIPLLLPEAGPPDGPDAARAARIADVEARFERFADGFAARD
                     PERPNILADMRYSLEVASFAPLWGIQVGGAVAGGDPVSWVDLAGDRRASLRRMWDDLR
                     RH"
     gene            complement(2046..3947)
                     /locus_tag="VFD06_00145"
     CDS             complement(2046..3947)
                     /locus_tag="VFD06_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN01972.1"
                     /translation="MRLPGFLRRSVRRKVTALVVATTLAALLVTALALVSYNVHDYRQ
                     SKLAEMRTQAEIIGRASAPALTFADRKEATRGLAMLEARPDVLQAALYAADGTLFASY
                     ARDGDSADIPVRWATLGEWIEQDRLDLVSPIVEDGQRLGTILLRARYGLRDRLLAYLV
                     ILGTVMAGALGAAVLLSAWLQRAVTEPIQGVADAARRVVDRRDFSVRAGRTTEDEIGM
                     LADAMNRMLADLEREIRERRDAEEALRVVDRRKDEFLATLAHELRNPLAPIRNALHLM
                     QAPGSDARVNAEARAIIDRQVRQMVRLVDDLLEVSRITTGNLALRRERVELRSVAAAA
                     LEAVDPIMRERGHRLTVDLPPAGLRIDADPTRMAQVFLNLLNNAAKFTPQGGRIDFSL
                     EVKDGEMIGRVRDNGVGIAADMLDPIFEMFAQADRSLERTTSGLGVGLSLSRKLIELH
                     GGRLEARSAGPNQGAEFTVRIPAAGAAGRDRGDGTAGPRFASSPRLVPARDASPSPLA
                     TAATEKKCRVLVVDDNQDFADTLGHMLKSLGHEVRVEHDGLAGLAAARTFRPDIAFLD
                     IGMPGLNGYDLARRLRAFDSTRGVVLIAVTGWGQANDRQRAYDAGFDDHVVKPLEMDR
                     LQSLLGRMG"
     gene            complement(3944..>4309)
                     /locus_tag="VFD06_00150"
     CDS             complement(3944..>4309)
                     /locus_tag="VFD06_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HZN01973.1"
                     /translation="VIGVIDAEEVRASLADIVRGREARGHPVEVRRLESGDATEGVHV
                     LFIGRPASEPLLRLVGRPALLLVTEAEGALDRGAMINLLLVDDRVRFEVAPQVAERSG
                     LHISSRLLVLAQSVRTVAP"
BASE COUNT          667 a         1514 c         1593 g          535 t
ORIGIN      
        1 gcgggacgcg acgtccaccg tttcgcggac ctgggcgcgg cggaggaggt cgagccgctc
       61 gccggcggtc acgggggaca ggcggagcgg ttcgtcgccg gtcgcggaca ccgaaacgca
      121 caggagcagg agaatcccga cgcaccgtgc agtgatcccc ggacaagcac gagcccgccg
      181 cgccatgaag ggtcctcttt gcgaagaatc gacgcggggt cctgcaacgg caatgccact
      241 tcgaagcgac taggggctcg cgcgccgccg tccttcgcgt ttgacgcgca ggctctccac
      301 ccactcacgc gcgaagtcgc gctgttggcg catgttgacg agcgcggtca cgaccatggc
      361 gatgagtccg ccgaggctcg cgagcaccat gtccttctgc gcgtcccacg ggtctccctg
      421 ggtcccgaga taggcgaccc cgagatcgcc gccgaagatc tccgcggccg cccactcgat
      481 cagctcgaag agcatcgaga aggccatggt catgtcgagc ggcaggtagt agccccagaa
      541 gccgcgcgcc gacgccacgc gcacgaagac ctcgcgcatg gggtacgcga gcaggagccc
      601 gaaggcgaaa tgcccgaggc gatcgaaatg gttccggccg gcgccgaggc tcacgccgat
      661 cccctgcagc cagtcgtggt acggcacttt ggcgtaggtg tagtgagcgc cgatctcgtg
      721 caggcacagg aacaggaaga tcagggtgta ggagacgcgc gagaagcgga accagcggtc
      781 ggtgacgacg agcaccgcca gcgccacgac gacgagggcg ttctccagca tccagtccgc
      841 ccggtcgacc ggcgcgatgg cgagcgccag ccaccagagg gcgaacaatc cgagcaggac
      901 gaggacgtag gcgcggtggg agagggtcac gtgttcctcc ggatggccgg gatgccggtc
      961 agtgccgccg cagatcgtcc cacatccggc gcagcgaggc gcgccggtcg cccgcgaggt
     1021 cgacccagga gaccggatcg cccccggcga ccgcgccccc cacctggatt ccccacagcg
     1081 gcgcgaacga agccacctcg agcgagtagc gcatgtcggc caggatgttg gggcgctccg
     1141 gatcgcgcgc ggcgaagccg tcggcgaacc gctcgaagcg cgcctcgacg tcggcgatgc
     1201 gcgccgcgcg cgcggcatcc gggccatcgg gcggtccggc ctccggcagg aggagcggga
     1261 tcgaggtgcc gggacggaac cgcggcgccg aagcgaaggg gacgcggcct ccgtcgacgt
     1321 ggatccgccc ctccgagaca tagaggccgc gccagacgac gaggttcatc ggcgtcggca
     1381 gcgcgcgggc ccgctcgacg acgtgaccgc gggaggcggc gaggcggtgc acctcctgaa
     1441 gtgcggtggc gtgctgccgc gcgccgaggc cgaggtaggc ggcgcacagc atcagcgcgg
     1501 cgagggccgg cgcgcgacgc aggcgccagg cggcgagaag cgcgccgacg aggagcggca
     1561 gcgtgaagag cggatcgacg atcgcgatga cgtcccaggc ggcgcgcacc ccggagaacg
     1621 gcaggagcag cgaggtcccg tagctcgtgc acgcgtcgag caggccgtgc gtggcgtagg
     1681 cgagcgtcgc ggccaggagc gtccgcttcc actgcgcgcg tccgcccgct cccaggagga
     1741 acggcagcgc cgcgacgagg cccccgagcg gggaggcgat cagcgcgtgg gtggcgtggc
     1801 gatgcacttc ccacggcagc gccggatcgc tgacgagcct caggagcacg tcggcgtccg
     1861 ccagcgtccc ggccgcggcc cccagcagga gcgccttgcg cccctccgag gcagggagga
     1921 gggcctgcgc cacggcggcg cccagaagtc cgtgtgtcag gggatccacg ccgatcagga
     1981 taccggaaga acgggatccc gacagccctg gaccgcttgt ccggcgcgga tttccggccg
     2041 gaccctcagc ccatgcggcc gagcagtgac tgcagccggt ccatctcgag cggcttcacg
     2101 acgtgatcgt cgaagccggc gtcgtacgcg cgctgccgat cgttggcctg gccccagccg
     2161 gtgacggcga tgaggacgac gccgcgggtg gaatcgaagg cgcgcagccg gcgggcgagg
     2221 tcgtagccgt tcagccccgg catgccgatg tcgaggaagg cgatgtcggg gcggaaggtg
     2281 cgggcggccg cgagcccggc gaggccgtcg tgctcgaccc gcacctcgtg tccgagcgac
     2341 ttgagcatgt ggccgagggt gtcggcgaag tcctggttgt cgtccaccac caggacgcgg
     2401 cacttctttt cggtcgccgc cgtggcgaga ggggacggcg atgcgtcgcg cgccgggacg
     2461 agacgcggcg aggacgcgaa ccgcggcccc gcggtcccgt ccccccgatc ccttccggcc
     2521 gccccggccg ccgggatgcg cacggtgaat tccgccccct gattcggccc cgcgctccgg
     2581 gcctcgagcc gcccgccgtg cagctcgatc agcttgcggc tcagcgagag ccccacgccg
     2641 agacccgacg tggtgcgttc gagggagcga tccgcctgcg cgaacatctc gaagatgggg
     2701 tcgagcatat cggcggcgat gccgaccccg ttgtcgcgca ccctcccgat catctcgccg
     2761 tccttcacct cgagggagaa gtcgatgcgg cctccctggg gcgtgaactt ggcggcgttg
     2821 ttcagcaggt tcaggaagac ctgcgccatc cgcgtcggat cggcgtcgat gcgcaggccc
     2881 gccgggggga gatcgacggt caggcgatgg ccccgctccc gcatgatcgg atcgaccgct
     2941 tcgagggccg cggcggccac cgaacgcagc tcgacccgct cgcggcgcag cgcgaggttg
     3001 ccggtggtga tgcgcgacac ctcgagcagg tcgtccacca gccgcaccat ctgccgcacc
     3061 tgccggtcga tgatggcgcg cgcctcagcg ttgacgcggg cgtcgctgcc gggcgcctgc
     3121 atcaggtgga gcgcgttgcg gattggcgcc agtgggttgc gcagctcgtg cgccagcgtc
     3181 gccaggaact cgtccttgcg ccggtcgacg acgcgcagcg cctcctcggc gtcgcggcgc
     3241 tcgcggatct cccgctcgag atcggcgagc atgcggttca tcgcgtcggc cagcatgccg
     3301 atctcgtcct cggtggtgcg tccggcgcgc accgagaagt cgcgccggtc gacgacccgg
     3361 cgggcggcgt cggccacgcc ctggatcggc tcggtgacgg cgcgctgcag ccaggcggac
     3421 aggagcacgg cggcccccag cgcccccgcc atcacggtgc cgaggatgac gagatacgcc
     3481 agcaggcgat cgcgcaggcc gtagcgggcg cgcaggagga tcgtcccgag acgctggccg
     3541 tcctcgacga tcggggagac gaggtccagc cggtcctgtt cgatccactc cccgagcgtc
     3601 gcccagcgca ccggaatgtc ggccgaatcg ccgtcgcgtg cgtacgaggc gaagagcgtg
     3661 ccgtcggccg catacagcgc cgcctgcagg acgtcggggc gggcctccag catcgccagg
     3721 ccgcgtgtcg cctccttgcg atcggcgaag gtcagggcgg gcgcgctggc gcggccgatg
     3781 atctcggcct gggtgcgcat ctccgccagc ttggattggc ggtagtcgtg gacattgtag
     3841 gagacgaggg cgagcgccgt gacgaggagc gctgcgagtg tcgtcgccac gaccagcgcc
     3901 gtgaccttgc gacgcaccga gcggcggagg aacccgggga gtctcacggc gccaccgtcc
     3961 tgaccgactg cgccagcacc aggaggcggc tgctgatgtg caggccgctg cgctcggcca
     4021 cctgcggcgc cacctcgaac cgcacccggt cgtcgaccag caggaggttg atcatcgccc
     4081 cccgatcgag cgccccctcc gcttcggtga cgaggagcag ggcgggccgg ccgacgagcc
     4141 ggaggagcgg ctcggaagcg gggcggccga tgaacagcac atgcactccc tccgtcgcat
     4201 caccgctctc gaggcgccgc acctccaccg ggtgcccccg cgcctcgcgg ccccggacga
     4261 tgtcggcgag cgacgcccgc acctcctcgg cgtcgatcac gccgatcac
//
LOCUS       DASRIQ010000010        19401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1826527, whole genome shotgun sequence.
ACCESSION   DASRIQ010000010 DASRIQ010000000
VERSION     DASRIQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 19401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19401
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826527"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFD06_00155"
     CDS             <1..1182
                     /locus_tag="VFD06_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01974.1"
                     /translation="KEEAAALAAEVAPIVEEIRGAKFKHPVPVEIVDDQAARAHFKTR
                     LQEHWPEEVRRAEETVDAHLGLIPPQTDLEEEVFDVLEEQAGGYYDPDRDTFFVLGDM
                     PRTAAPIIVAHELTHALDDQRYGIDRLLSEADGDDDRAGALESVVEGSGTVVMTVFIH
                     RQIVAGRLDPKALLAFQESEVGKAERLKTSPLFVQRTLIAPYILGMAFLLRGELIRIQ
                     TGGITPSDLDRAFSDPPRSTEQILHPEKYWDPKQRDLPRLVTLPDLSADLGPGWSRRG
                     EGNFGELTLALLTGLDPGDPTSPDRALERWTNAASSGWGGDRWNLYRNGEQAITVLAT
                     SWDTTNDAREFETGLALPEGTFVKRKGDAVVIVAGDAAEKGKKIADRAISGLKVGKPG
                     A"
     gene            complement(1310..1717)
                     /locus_tag="VFD06_00160"
     CDS             complement(1310..1717)
                     /locus_tag="VFD06_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01975.1"
                     /translation="MRFGRRATGTGLLLGLLVAGGSARADAPAVADPGQIRLVVRHAL
                     DAGRLVVRVGETAFFSAPIASLRKASTGEVERLLSIPSGLQTVSVELRDGAGRIVDRR
                     QVRAFVVPGTAAILDVVAPGAGEALRLDWRATP"
     gene            1932..3959
                     /locus_tag="VFD06_00165"
     CDS             1932..3959
                     /locus_tag="VFD06_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HZN01976.1"
                     /translation="MAVNKTAAKTLGIEPEIFLALYEKMLRTFYVEEMGRLFVRAGKC
                     SFYASTRGHEKLQIACAMLLAPGKDWFFPYYREKALMIGLGMPLEHVFQGLLSRDGDP
                     NSLGRNMSEHYSSRELRVVSPTACTGTQFLQAVGLARAVKADKKDEIVYVSGGEGSTS
                     EGEFFEALNKAQRDLLPVMFVIQNNGYAISVPQEQQTHSRLDEVARGFGMKSVRVDGT
                     RFTEMHRTLHPLVTAMRRGAGPLFVEAMVVRLDSHSSSDDQTKYRAEQDMEEARRKDP
                     MTHTEMRIRQMGLLTDKEIEEWRTRVKAEVEAAARAVDALPFPPEASATADVFSPDRV
                     IVEETDPTPASDKPVTMVEAINHGLMEEMKRNPKLVMWGEDIQDPKGGVFGVTRGLTE
                     AFPGRVENSPLAEATIVGLAKGMAIGGYKPIIEIQFADYSFPGYMQMRNEIPTLRWRS
                     GGAWTCPLVVRIATGGYIRGGPFHSQCPETLYAHTPGWYIAYPSNAADAKGLIKAACR
                     MDDPVIFFEHKGLYRQVYSKSPEPGADYVLPFGKARVVREGDDLTVVAWGRTVHMAQQ
                     ALGALASEGQEPSVEILDLRTIVPMDLDAVLTSARKTGRVLVAHEAPMLAGMGAEVAA
                     RIVDAAFDHLDAPVRRVAARDSFVPFAPNLEAAVLPSVEEIAAAIKDLMQY"
     gene            4007..5275
                     /locus_tag="VFD06_00170"
     CDS             4007..5275
                     /locus_tag="VFD06_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN01977.1"
                     /translation="MTNDPVLTLPSPSLILVIGPAAAGTATFCRRNFPPSAIVSWDEC
                     RDAVGARAQGRRDDAAVALARERLEARLRRRRLAVLDGAALQPEARGEARAIAARQHL
                     PVIAVVIEATPALPSLRGEGFDAVHRVRGAAAVASTRVALAPLPCDRSADRGPFDIIG
                     DVHGCADELLSLLRRLGYRRRAGRWRHPRGRRALFLGDLVDRGPRVIEAATIAIDMTA
                     HGAALAVPGNHDVELAALLRGGGGTPGPGTILSLAQLAAAPSSVRRRFHRRFTAYVDR
                     LPAHLLLDGGRLAVAHAGLLAEHIGRESEAVRRFAVHGVTLQRVDRYGLPIRVHWATA
                     YRGRTFLVYGHTAVRAPESVGGTLNIDTGCCYGGALTAFRWPEREVVQVRASRVHYRS
                     PRTRGLRLGIRAETRASVARGGRPLRPTSS"
     gene            complement(5262..5711)
                     /locus_tag="VFD06_00175"
     CDS             complement(5262..5711)
                     /locus_tag="VFD06_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01978.1"
                     /translation="MKYRQPGYRDSEYKDEREKKHRDNDGPRPPRGPRGMEREARLVL
                     RCFQCGHQEPATDVVETGARCPTCTAAVHCCRNCVHFDSGARFECREPIPAKVMSKIV
                     ANECASFRPSTVLDATGRRIAAGPPPPSGEPAMSPGRAAFEALFKKK"
     gene            5831..7387
                     /locus_tag="VFD06_00180"
     CDS             5831..7387
                     /locus_tag="VFD06_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN01979.1"
                     /translation="MNRTQIACALLLLAALLPAPAAGAPPARRPATPKPPAAAAPAPA
                     AKTAEVPIPQEDGFERSVVRIINYSQRGDWFSPWDVTGVRASSGTGFVIDGGVLLTNA
                     HVVSDARLLLLYLHGDPTPHKARAVMLGHDCDLALVRPEEEDLLAAVPVLPLGGLPPL
                     RSAVETYGYPAGGDQISSTRGVVSRIETQVYAHSGVDRHVAIQTDAAINPGNSGGPMI
                     QNGKVVGVAFQGTDMLENTGFGIPVEVIRHFLDDAKDGTYDGYPDLAILESNLENPAA
                     RRRAGMTQGETGVRVDFVNSGGSAEGQLQTGDVLLAVDGIPIANDGSVLMEGLRFDYG
                     LMVDRHQIGETLKLRILRDDERRDLAFPLVAHPGTSRYANQYDVLPRYYIYAGLVFEP
                     LNVEMLETTGRDWVVSADKRLVQEQIFRPLSDPKALLREPVVLLRRLDDPVNINIAWY
                     RNQLVTKANGKEIDSLGGLIEAIEANKAAFHVLELGEPGRFIVLDRKGSDEASPKILE
                     RYGVLKDRNP"
     gene            7384..8913
                     /locus_tag="VFD06_00185"
     CDS             7384..8913
                     /locus_tag="VFD06_00185"
                     /inference="COORDINATES: protein motif:HMM:NF036494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01980.1"
                     /translation="MRGTPRFGTAAILAAAATIALPGAAWARPAATRQAFETGIVGLT
                     ISAQGWDQDRPWAKSAPQSRMALATVVEGPYLLTSAQMVSDATLIEAEKLGGSVRVQA
                     KVVLADPEVDLAILSVDTPGFFSDLKPVPMADAMPTEGSVQSVRWRLRQMEVSTSRVS
                     RIEVQVASLGGLEHPFLLVTTDLRGGGWAEPVFTDGKFIGLTVSQDEQVARVLPIDMI
                     SEFLSMARGAKPYPGFSNLGVRWQINEDPVLTASLRLPGDPRGVLVTGANWGGSACGV
                     LRPRDLLLALDGHDIDASGYYRHPRYGLLRFTTITTDGHRAGEVLKAEVWREGKKMML
                     DVPLKATHTAGDLIPDRVPDRAPPYLIAGGLVLRELDGSYMRSWGDDWRRKAPIRLQL
                     RYWLDSKDQEPGARRILILSSVLPAAYNLGYHGLSDVAVTEINGRKVDSIAAASEALK
                     HPEGEFHRLVLEPNGQMAQVILDAGTFDAATSEILESYRVPAAGREAPSPPDLGAACG
                     D"
     gene            9024..10091
                     /locus_tag="VFD06_00190"
     CDS             9024..10091
                     /locus_tag="VFD06_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZN01981.1"
                     /translation="MNRPLRTIFPAVVLCCAATVFACGAATGETGKAAKKGASPAAAA
                     DGVAAKVGDKTITVAELDTKAAAQLMKVRQQEFEVRQQALDAMINDELLEREAKAKGV
                     TREKLEETEIAAKAPEPAQAEIDAYYEANKARMGTQTKEQIGPQIAAMLKQQKMASVK
                     ADYLKGLRQKYGVKVMIEPPRVQVSADDDPAKGGGTSAPVTIVEFSDYQCPYCSRAEN
                     VVDEVMKKYGEKVRLVYRDYPLSFHQNAETAAMASECAEEQGKFWEMHKAMFGNQQKL
                     TAADLVETAGTIGIDKDKFKTCLDSGQFRPEVQKDFQDGQKAGVTGTPTFFINGVMIV
                     GARDVASFSDIIDRELDRSGK"
     gene            complement(10252..11340)
                     /locus_tag="VFD06_00195"
     CDS             complement(10252..11340)
                     /locus_tag="VFD06_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01982.1"
                     /translation="MNPLTPPDDERDDPDAPDAPDDPDFGAAQGDPGDDRHDDDDHDH
                     DDEEDDSLRDEIEPGYQWTFAEQEGRLAEEYPRHMQILDDLEAREILALDLARAGDYL
                     LWAAAQSLEEQGWTGEAMALLRRLDRGEGGHPALAYADIRLRLHDLLRERGEYGEALQ
                     VLERVERDAPERRDACRERHAEVLVLTGRKEEGLRMYEKVLRAVPDDPWPPLNAAWAL
                     IRAGDYDLALSWIGRGERAANRLEDEEEAQPALNEIERLRNEARIRRERRQRATGEGP
                     ESLRQAILADLDAEEVRLTREPPRGETDRDAALARLVELHRRASAAWDDAVERHDEAA
                     ISTFDDLQWEVVEVAERFGLRIPGTEGD"
     gene            complement(11337..14360)
                     /locus_tag="VFD06_00200"
     CDS             complement(11337..14360)
                     /locus_tag="VFD06_00200"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HZN01983.1"
                     /translation="MKARARGVVAAILVLPLAASPPAAAVGAAAPVAANATGDAVAAA
                     RQAQIAYFRNDPFSPLRGVRRFDFPEPKEENGASSALLGSGSEVDLRLDAPGIPGRLL
                     RMTVLAPEKEGAPFRFQLERLAEKDDLRLAGEPFRETERIVPEETRVEAGPFALRPYV
                     QGGSGIVIVFDARRGTGGGFTAPEFFPYDARWSFRLPLHRHEHPETVSVATSLGRTKE
                     YRRAGWFEVSAPGGAGGPRTLKVQAYQPLFTAAPDETLSILFTDATTGKESYATGRYL
                     DLGAPKDGLYDVDFNRAYNPLCAYTTVYNCPIPPRENALPVPVRAGEKAYPDHAAKDR
                     AAAGQDTATFVPAAAREDPIRAGASPPGDLFASGLRDFLDWAHPAAEGCVAIDRIEIV
                     SRFAGGLRARYEVNCGGGPRLLEGILSTREAPGVWQVAAGFEADEKDVSEAIRTRLLR
                     IPRELIEGPGARRTKGPDALLPPPDNPGLAGPLDAVVPPVPVREAPPTFPEEAGRARL
                     IGEARVELLVDVSPRGEPRRARTLRGPDPDLGMRASANAAVNGWRFRPASLGGHPVRY
                     FAPVEITFTGLPPESRAWAHRALFDLQSLAFDQTGPAQEGVKRLRAGAPVEDVSPESA
                     LDADWGLVAAADLPGVLRQALHEARVGSWTDPVAADGRQYVMRKSGEVYYAILPPGNG
                     PEVQYQIVHQRGVSEGEALRLAIDADVADFMAERHRRDYMNEAARLMGIRQSRVELGQ
                     LVIHTDALNADETAVLGQVVNAAVRAHQEFWADVTTLRLFRQPVYVYAFGRRSDHIRV
                     QQLWRGRRPAGAATAVGEYLPASRILAFPCEAAGGHVPIPIVVHEAIHMLDYERVYPP
                     RAQPSKWFEEGLANYFGFSRLDGDLKVDAGAIRASASIVIGSARVQFDPRAELREHLR
                     LTRVEGPMPLEPLLAAGPEDELWAGGRSARAYGAAWTFVHFLRHSDRGRRAAAFSRYA
                     AREAGGQGGLAAFKEIFGNDLAALEAAWHDYEATL"
     gene            complement(14357..14923)
                     /locus_tag="VFD06_00205"
     CDS             complement(14357..14923)
                     /locus_tag="VFD06_00205"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZN01984.1"
                     /translation="MATRCGWAGNDPLYIAYHDEEWGVPVHDDQRLFEFLVLEGAQAG
                     LSWITILRKRAAYRAAFAGFDPARVARFDARKVARLLKDPAIVRNRAKVASAVGNARA
                     FLKVQKEFGSFDAYIWRFVDGRPKCNVWRTLKQVPAETDESRAMSRDLRGRGFNFVGP
                     TICYSHMQATGMVNDHIVDCFRHREVGR"
     gene            complement(15011..17308)
                     /locus_tag="VFD06_00210"
     CDS             complement(15011..17308)
                     /locus_tag="VFD06_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN01985.1"
                     /translation="MSIQPNAALAALLSGTAILLLVSKCTRAAAFLGAVVAGIGASVL
                     FQCLVGIELGIDDILLFGREWGQRGVLVPGRMGLPGAVSWTILGTALWLASTRTRARA
                     AVPGLACLAAGISFLSIVGYLYRADELFLLPRLTIIALQSSTFILAVALALLLSLPEF
                     PPVRRIIEESEAGIILSRAVPLVIIASIACGFLAIVGLQAGLYDPRFQAALRTFSEVA
                     FFLAVLWWTARAVERQARRTREQARLLELTSDAVFVRGPGDRIETWNHGAQELYGWTQ
                     EEALGRSSHALLRTESTEPLESIEAALRREGRWDGELVHTTRDGRKVIVSSRWVLDRR
                     PAGPDAVLEAGNDITIHKAYEEERQRLLDNERRAHHESERANRIKDEFLATLSHELRT
                     PIHVILGWARLLDRDDPDPSLVREAVAVISRNARTQAELVADLLDMNRILRGNVKLEP
                     VLVRTSDLVVEAVEAIGPLAAEKGVEVRSDAGTVGTVRGDALRLRQVLGNLLSNAIKF
                     TPPGGLVRVQVEQAEGHAEIAVADSGIGIPHEFLTEVFEPFRQGDTSSTREYGGLGLG
                     LAIVRQLVRAHGGSVRVESDGIGRGATFTIRLPLAIEEAPSARPDSESPAGVDAAPAR
                     TDSALVGATVLVIDDQEDARRFLALHLQRCGASVVTAASATEGLRLLDSVTPDIVLCD
                     IAMPGQDGYAFIGEVRRRNMAIPALALSAYARVEDRDRALRVGYQGHIAKPIDPAELI
                     SVVTRTMRETPIDEATAKDGLLPAI"
     gene            complement(17570..18316)
                     /locus_tag="VFD06_00215"
     CDS             complement(17570..18316)
                     /locus_tag="VFD06_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZN01986.1"
                     /translation="MPAETLRVLIVDDEALARRGLRRDLAALEGIDVVGESADGFEAV
                     KAAAELKPDLVLLDIRMPRLDGFEVLSLLDPGIAVVFVTAHDDRAVQAFDANAVDYVM
                     KPVDPERLARAVERARERRRRREPMPVAAIGAAARPPGEPLGRILVRDGARVHVIPMD
                     DVDCVEAQDDYIAVHAGGRAHLKQQTIGELEAALDPRRFVRIHRSWILNIDRLDRIEL
                     YAKDSRVAILKDGRRLPLSRAGNLRLRELL"
     gene            complement(18306..>19401)
                     /locus_tag="VFD06_00220"
     CDS             complement(18306..>19401)
                     /locus_tag="VFD06_00220"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN01987.1"
                     /translation="AVCHLLRDPLAPVRIGLFLTIALLLGGLAATILALGGGAGWEEA
                     LLLGLPLGLVEGAIVLAARYPCQSAPLRASGMTRALVTQATAALASAGAWVVLGGILA
                     RMLETLPRYGGAALRLRGSAPMLVVVGALVFLLAAAAHYLILALEAARAAEVRGMEAR
                     VLAREAELRALRAQVDPHFLFNSLNSIATLTSEDAGAARRMCLLLAGFLRRSLALGGR
                     DRVTLGEEIALVDEYLAIEKVRFGSRLEVAREVDAGCEAALIPPLLLQPLVENAVRHG
                     VAQRIEGGTLRLRAERRGAGEIAVTIDNPRNPERSGRRGAGLGLENVRGRLRSLYGDR
                     ARLEVATPPEAFVVTVTLPYVAATETSLAG"
BASE COUNT         2872 a         6972 c         6889 g         2668 t
ORIGIN      
        1 aaggaggagg cggcggcgct ggcggcggag gtggcgccga tcgtcgagga gatccggggg
       61 gcgaagttca agcacccggt gccggtggag atcgtcgacg accaggcggc gcgggcgcac
      121 ttcaagaccc ggcttcagga acactggccg gaggaggtgc gccgcgccga agagacggtg
      181 gacgcgcacc tcgggctgat cccgccgcag accgatctcg aggaggaggt gttcgacgtc
      241 ctcgaggagc aggcgggggg ctactacgac ccggaccgcg acaccttctt cgttctcggc
      301 gacatgccgc gcaccgccgc cccgatcatc gtggcgcacg agctgaccca cgcgctcgac
      361 gaccagcgct acggcatcga ccgcctgctg tcggaggcgg acggggacga cgatcgcgcc
      421 ggcgcgctcg aatcggtggt cgagggttcg ggaacggtcg tcatgaccgt cttcatccat
      481 cgacagatcg tcgccggccg gctcgacccg aaggcgcttc tggccttcca ggagagcgag
      541 gtcggcaagg cagagcggct caagacctct cctctcttcg tgcagcgcac cctcatcgcc
      601 ccctacatcc tcggcatggc gttcctgctg cgcggcgagc tcatccgcat ccagaccggc
      661 ggcatcaccc cctcggacct ggaccgcgcc ttctccgacc cgccgcgatc gaccgagcag
      721 atcctccatc ccgagaagta ctgggacccg aagcaacgcg acctgccgcg cctggtgacg
      781 ctccccgacc tgtcggccga tctcggtccg ggatggtcgc gccgcggcga agggaacttc
      841 ggcgagttga cgctcgcgct cctgaccgga ctcgaccccg gggatccgac ctcgccggat
      901 cgggcgctcg agcggtggac caacgccgcc tcgagcggct ggggcggcga ccgctggaac
      961 ctctaccgca acggcgagca ggcgatcacc gtcctcgcga cctcctggga cacgacgaac
     1021 gacgcccgcg agttcgagac ggggctggcg ctccccgagg ggacgttcgt gaagcgcaag
     1081 ggggacgcgg tcgtgatcgt cgcgggagac gcggcggaga aggggaagaa gatcgccgat
     1141 cgcgcgattt ccgggctcaa ggtcgggaaa ccaggagctt agcgcccggt ccccgggcag
     1201 atcatgccgt tcgagggggc gccgttgccc ggcgcgcgga tcccggacgg tgccggcggc
     1261 cgggccggcg gcttcggcgg agggagcggg cgctctttgc ggacgcgcct catggcgtgg
     1321 ccctccagtc gagccgcagc gcctcgccgg ctccgggggc gacgacgtcg aggatggcgg
     1381 cggtccccgg gacgacgaag gcgcgcacct gccgccgatc gacgatccgc ccggcgccgt
     1441 cgcgcagctc gacgctcacc gtctgcagtc cggaagggat cgacaggagc cgctcgacct
     1501 ctccggtgga agcttttcgc agcgaggcga tcggggcgga gaagaacgcc gtttcgccca
     1561 cgcgcacgac cagccgcccg gcatcgaggg cgtggcgcac gaccaggcgg atctggccgg
     1621 gatcggccac cgcgggggcg tccgcgcgcg ccgaccctcc ggcgaccaga agcccgagca
     1681 ggagtcccgt tcccgtcgca cgccgcccga accgcatccc gcacctcccg atccgcaagg
     1741 cgccgaaagg cgtcgcggcc gcggctgaat atgagcaccg ggacccccgc ggtcaaggcg
     1801 cgtaaacgcc tgcggcgcag cacctttcac gcttgcgtca tttcattctg gaaacccggc
     1861 cctgtcagta gggttccgtc ttggagcccc gccctcgcgg gtgctagcat ggcgccgatc
     1921 ggagggcctg catggcggtg aacaagacgg cggcgaagac gctcgggatc gagccggaga
     1981 tcttcctcgc cctgtacgag aagatgctgc ggacgttcta cgtcgaggag atggggcgtc
     2041 tcttcgtgcg ggccgggaag tgctcgttct acgcctcgac ccggggccac gaaaagctgc
     2101 agatcgcctg cgccatgctc cttgcgcccg ggaaggactg gttctttcct tattacaggg
     2161 agaaggccct gatgatcggg ctcgggatgc cgctcgagca cgtcttccag gggctcctgt
     2221 cgcgcgacgg cgacccgaac tccctcggcc gcaacatgtc cgagcactac tcctcgcgcg
     2281 aactgcgcgt cgtctccccg accgcctgca ccggcaccca gttcctccag gcggtcggcc
     2341 tggcccgcgc cgtcaaggcg gacaagaagg acgagatcgt ctatgtctcc gggggcgagg
     2401 gatcgacctc cgagggggag ttcttcgagg cgctcaacaa ggcgcaacgc gacctcctgc
     2461 cggtgatgtt cgtcatccag aacaacggct acgcgatcag cgtgccgcag gagcagcaga
     2521 cccattcgcg cctcgacgag gtggcgcgcg gcttcggcat gaagtcggtg cgggtggacg
     2581 gcacccgctt caccgagatg caccgcacgc tgcacccgct cgtcaccgcg atgcgccgcg
     2641 gcgcgggacc gctgttcgtg gaggcgatgg tggtgcggct cgactcgcac tcgtcgtcgg
     2701 acgaccagac caagtaccgc gccgagcagg acatggagga ggcgcgccgc aaggatccga
     2761 tgacccacac ggagatgcgc atccgccaga tgggcctcct gaccgacaag gagatcgagg
     2821 agtggcggac gcgcgtcaag gcggaggtgg aggcggcggc acgcgccgtc gacgccctgc
     2881 cgttcccccc ggaggcgagc gcaacggccg acgtcttctc tcccgaccgg gtgatcgtgg
     2941 aggagaccga cccgacgccg gcgagcgaca agccggtgac gatggtcgag gcgatcaacc
     3001 acggcctcat ggaggagatg aagcgcaacc cgaagctcgt catgtggggg gaggacatcc
     3061 aggacccgaa gggcggggtc ttcggcgtca cgcgcggtct caccgaggcg ttccccgggc
     3121 gggtggagaa ctcgccgctg gcggaggcga cgatcgtcgg gttggcgaag gggatggcga
     3181 tcggcggata caagccgatc atcgagatcc agttcgccga ttactccttc cccggctaca
     3241 tgcagatgcg caacgagatc ccgacgctgc ggtggcggtc gggcggggcc tggacctgcc
     3301 cgctcgtcgt gcgcattgcg acaggaggtt acatccgcgg cggtccgttt cactcccagt
     3361 gccccgagac cctctacgcg cacacccccg gctggtacat cgcctatccg tccaacgccg
     3421 ccgacgccaa ggggctgatc aaggcggcgt gccgcatgga cgatccggtg atcttcttcg
     3481 agcacaaggg gctctaccgg caggtgtaca gcaagtcgcc ggagccgggg gcggactacg
     3541 tgctgccgtt cgggaaggcg cgggtggtcc gggaggggga cgacctgacg gtcgtggcgt
     3601 gggggcgcac cgtgcacatg gcgcagcagg cactcggggc gctcgcctcc gaagggcagg
     3661 agccctcggt cgagatcctc gacctgcgca ccatcgtgcc gatggacctc gacgcggtgc
     3721 tcacctcggc gcgcaagacc gggcgggtgc tggtggcgca cgaggcgccg atgctggccg
     3781 ggatgggggc ggaggtcgcg gcgcgcatcg tcgacgccgc cttcgaccac ctggacgcgc
     3841 cggtgcggcg cgtggcggcg cgcgactcgt tcgtgccgtt cgccccgaac ctggaggcgg
     3901 cggtgcttcc gagcgtcgaa gagatcgccg cggcgatcaa ggatctgatg cagtactgac
     3961 gacggtcgcg ccgcgggggc ggggcgcggc gcgtttcgag ggagtcgtga cgaacgaccc
     4021 ggtcctgacc ctcccatccc cctcgctgat cctcgtgatc ggtccggccg ccgccggcac
     4081 ggccacgttc tgccggcgca acttcccgcc ctcggccatc gtctcgtggg acgagtgcag
     4141 ggacgccgtc ggcgcgcggg cccagggccg gcgggacgac gcggcggtcg cgctggcccg
     4201 cgagcggctg gaggcgcggc tgcgccggcg ccggctggcg gtgctcgacg gcgcggccct
     4261 ccagccggag gcgcgcggcg aggcgcgggc gatcgccgcc cggcagcacc ttccggtgat
     4321 cgcggtggtc atcgaggcga cgccggccct cccctcgttg cgcggggagg ggttcgacgc
     4381 ggtccaccgc gtgcgcggag ccgccgcggt cgcctcgacc cgcgtggcgc tcgcgcctct
     4441 cccctgcgac cggagcgcgg atcggggacc gttcgacatc atcggggacg tgcacggctg
     4501 cgccgacgag ctgctgtcgc tcctgcgccg gctcgggtac cgccgccgcg ccgggcggtg
     4561 gcgccatccc cgagggcggc gggcgctctt cctcggcgac ctcgtcgatc gcggaccgcg
     4621 cgtcatcgaa gccgccacca tcgccatcga catgaccgct cacggcgcag cgctggcggt
     4681 tccgggcaac cacgacgtcg agctggcggc gcttctgcgc ggggggggcg gaacccccgg
     4741 tccggggacc atcctctcgc tggcgcagct cgccgcggcg ccctcctccg tgcggcgacg
     4801 cttccaccgc cgcttcaccg cctacgtcga tcggctgccg gcgcacctgc tgctcgacgg
     4861 cggacggctg gcggtggcgc acgccgggct cctcgccgag cacatcgggc gggagtccga
     4921 ggcggtgcgc cgcttcgcgg tgcacggcgt caccctccag cgcgtcgacc gctacggcct
     4981 gccgatccgg gtgcactggg cgaccgccta ccgcggccgg acgttcctcg tctacgggca
     5041 caccgcggtg cgcgcgccgg agtcggtcgg cgggacgctc aacatcgaca ccggatgctg
     5101 ctacggcggg gcgctgaccg ccttccgctg gccggagcgc gaggtggtgc aggtgcgggc
     5161 gtcccgcgtg cactaccgct cgccgcgcac gcgcggcctg cggctcggga tccgggccga
     5221 gacgcgcgcg tcggtggcgc gcggcggccg gcccctccgg cctacttctt cttgaagagc
     5281 gcctcgaagg cggcccgacc cgggctcatc gccggctccc cggacggggg cggcggcccg
     5341 gcggcgatgc gccgcccggt ggcgtcgagg acggtggacg gacggaagga ggcgcactcg
     5401 ttggcgacga tcttcgacat caccttggcc gggatcggct cgcggcactc gaagcgggcg
     5461 ccggaatcga agtggacgca gttgcggcag cagtgcaccg ccgcggtgca ggtggggcag
     5521 cgggcgccgg tctccaccac gtcggtcgcc ggctcctggt gtccgcactg gaagcagcgc
     5581 aggacgagcc gcgcctcccg ctccatgccg cgcggcccgc gcggcgggcg cggtccgtcg
     5641 ttgtcgcggt gcttcttctc gcgctcgtcc ttgtactcgc tgtcgcggta gccgggctgg
     5701 cggtacttca tgcggacgct ctgcctcctg gaaagttgtg cgagggagaa tcgcattcta
     5761 gcatggccag gagcctgtcc cggctcctca cggctttgcc ggcggcgctt cgtcccgtag
     5821 aatgcgccgg atgaatcgga cccaaatcgc ctgcgcgctc ctcctgctcg ccgctcttct
     5881 tcccgcgccg gccgcggggg cgccaccggc gcgccggccc gccaccccga agccccccgc
     5941 cgccgcggct ccggcacccg cggcgaagac cgccgaggtc ccgatcccgc aggaggacgg
     6001 cttcgagcga tcggtcgtgc gcatcatcaa ctactcgcag cgcggcgact ggttctcacc
     6061 ctgggacgtc accggtgtgc gcgcctcgag cggcaccggc ttcgtcatcg acggcggcgt
     6121 gctgctgacc aacgcccacg tcgtgagcga cgcgcgcctg ctgctcctct acctgcacgg
     6181 cgatccgacg ccgcacaagg cgcgggcggt gatgctgggg cacgactgcg atctcgccct
     6241 cgtccggccg gaggaggagg acctgctcgc ggcggtcccg gtcctgccgc tcggcgggct
     6301 gccgccgctg cgctcggcgg tggagaccta cggctatccg gcgggcggcg accagatctc
     6361 ctcgacccgc ggcgtcgtct cccgcatcga gacacaggtg tacgcccact ccggcgtcga
     6421 ccgccacgtc gccatccaga ccgacgccgc catcaacccg ggcaacagcg gcgggccgat
     6481 gatccagaac ggcaaggtgg tcggggtcgc cttccagggg actgacatgc tcgagaacac
     6541 cggcttcggg atcccggtgg aagtcatccg ccacttcctc gacgacgcga aggacggcac
     6601 gtacgacggg taccccgatc tcgccattct cgagtcgaac ctggagaacc cggcggcgcg
     6661 ccgccgcgcc ggcatgacgc agggggagac cggcgtgcgg gtcgacttcg tcaactctgg
     6721 cggcagcgcc gaggggcagc tgcaaacggg agacgtcctg ctggcggtgg acgggatccc
     6781 gatcgccaac gacggctcgg tgctgatgga ggggctgcgc ttcgactacg gcctgatggt
     6841 cgatcggcac cagatcggcg agaccctgaa gctccggatc ctgcgcgacg acgaacggcg
     6901 cgatctggcc ttcccgctcg tcgcccaccc ggggacgagc cgctacgcca accagtacga
     6961 cgtcctgccg cgctattaca tctacgccgg gctggtgttc gagccgctca acgtcgagat
     7021 gctcgagacg accggccgcg attgggtggt gagcgccgac aagcgcctgg tgcaggagca
     7081 gatcttccgc ccgctgtcgg atccgaaggc gctcctgcgc gagccggtgg tgctgctgcg
     7141 gcggctcgac gatccggtca acatcaacat cgcctggtac cgcaaccagc tggtgaccaa
     7201 ggccaacggc aaggagatcg actcgctcgg cgggctcatc gaggcgatcg aggcgaacaa
     7261 ggccgcgttc cacgtcctcg agctggggga gccgggacgg ttcatcgtgc tcgaccgcaa
     7321 gggttcggac gaggccagcc cgaagatcct cgagcggtac ggcgtcctga aggaccgcaa
     7381 cccgtgaggg ggacgccccg gttcgggacg gccgcgatcc tcgcggccgc ggcgaccatc
     7441 gccctgcccg gcgcggcgtg ggcccgcccc gccgccacgc ggcaggcgtt cgagaccggc
     7501 atcgtcggcc tgaccatctc ggcgcaggga tgggaccagg accgcccctg ggccaagtcg
     7561 gccccgcagt cgcgcatggc gctcgccacg gtggtcgagg ggccctacct cctcacgtcg
     7621 gcgcagatgg tgtccgacgc gaccctcatc gaggcggaga agctcggggg atcggtgcgg
     7681 gtgcaggcga aggtcgtcct cgccgacccg gaggtcgatc tcgccatcct gagcgtcgac
     7741 accccggggt ttttctccga tctcaagccg gtgccgatgg ccgatgcgat gccgacggag
     7801 gggtcggtgc agtcggtgcg ctggcggctg cggcagatgg aggtctcgac atcccgcgtc
     7861 tcgcgcatcg aggtccaggt ggcttccctc ggcgggctcg agcacccgtt cctgctcgtg
     7921 acgaccgacc tgcgcggcgg cggctgggcc gagccggtgt tcacggacgg gaagttcatc
     7981 ggcctgacgg tgtcgcagga cgagcaggtc gcgcgcgttc ttccgatcga catgatttcg
     8041 gagttcctgt ccatggcgcg cggcgcgaag ccgtatcccg gattctccaa cctcggcgtg
     8101 cgctggcaga tcaacgagga cccggtgctg accgcctcgc tgcgcctgcc gggggatccg
     8161 cgcggcgtcc tcgtcaccgg tgccaactgg ggaggatcgg cctgcggcgt gctgcggccg
     8221 cgggacctcc tgctcgcgct cgacgggcac gacatcgatg ccagcggcta ctaccgccac
     8281 ccgcgctacg gcctgctgcg gttcaccacc atcacgaccg acggccatcg cgccggcgag
     8341 gtgctcaagg cggaggtgtg gcgcgagggg aagaagatga tgctcgacgt gccgctcaag
     8401 gcgactcaca cggccgggga tctgattccg gaccgggtcc ccgaccgggc tcccccgtat
     8461 ctgatcgccg gggggctggt gctgcgggag ctcgacgggt cgtacatgcg ctcctggggg
     8521 gacgactggc ggcgcaaggc gccgatccgg ctgcagctgc gctactggct cgactccaag
     8581 gaccaggagc cgggagcgcg gaggatcctc atcctctcgt cggtgctgcc ggccgcctac
     8641 aacctcgggt atcacgggct gtcggacgtg gcggtcaccg agatcaacgg ccgcaaggtc
     8701 gactcgatcg ccgcggcctc cgaggcgctg aagcaccccg aaggggagtt ccaccgcctc
     8761 gtcctggagc cgaacgggca gatggcccag gtcatcctgg acgcggggac cttcgacgcg
     8821 gcgacctccg agatcctgga gtcttacagg gtcccggccg ccggccggga ggccccgtcc
     8881 ccccccgacc tcggcgccgc ctgcggcgac taggagcctg tcggacaggc tcctcgcccc
     8941 cgccccggcc ccccgggatg ccggcccggg gcgactggtg tagaatctcc gcccccaaca
     9001 caaagactcg aggaggatac ggaatgaatc gccccctgcg cacgatcttc cctgccgtgg
     9061 tgctgtgctg cgccgccacg gtcttcgcct gtggcgcggc caccggagaa accggcaagg
     9121 ccgccaaaaa gggagcctcc ccagccgccg ccgccgacgg cgtcgccgcc aaggtggggg
     9181 acaagaccat caccgtcgcc gagctcgaca ccaaggcggc cgcccagctg atgaaggtgc
     9241 ggcagcagga gttcgaggtc cgccagcagg ccctcgacgc gatgatcaac gacgagctgc
     9301 tcgagcgcga ggccaaggcg aagggggtca cgagggagaa gctggaggag accgagatcg
     9361 ccgccaaggc gccggagccg gcgcaggccg agatcgacgc ctactacgag gcgaacaagg
     9421 cccgcatggg aacgcagacc aaggagcaga tcggcccgca gatcgccgcc atgctgaagc
     9481 agcagaagat ggccagcgtg aaggccgact acctcaaggg gctgcgacag aagtacggcg
     9541 tgaaggtgat gatcgagccg ccgcgcgtcc aggtgtcggc cgacgacgat cccgcgaagg
     9601 gcggcggcac ctccgcgccg gtgaccatcg tcgagttctc cgactaccag tgcccctact
     9661 gcagccgcgc cgagaacgtg gtggacgagg tcatgaagaa gtacggcgaa aaggtgcgcc
     9721 tcgtgtaccg cgactacccg ctctcgttcc accagaacgc cgagaccgcc gccatggcca
     9781 gcgagtgcgc cgaggagcag gggaagttct gggagatgca caaggcgatg ttcggcaacc
     9841 agcagaagct gaccgccgcc gacctggtcg agaccgccgg aacgatcggg atcgacaagg
     9901 acaagttcaa gacctgcctc gactccggcc agttccgccc ggaggtgcag aaggacttcc
     9961 aggacggcca gaaggcaggt gtcaccggga cgccgacctt cttcatcaac ggcgtgatga
    10021 tcgtcggggc gcgggatgtc gcgtccttct ccgacatcat cgatcgcgag ctggatcgct
    10081 cgggcaagta agaacacacc cgaaaagctc agacatcgca ccggggcgtg actccggcgg
    10141 cagacccgcc gtggcgatcg agggggaggg ctcgcgacga aggcagcgag ccctgacccc
    10201 gtacccggcg ggcctgcccc cggcctcccg gaccctggtg cctgaagtgg tctagtcgcc
    10261 ttcggtgccc gggatgcgca gaccgaaccg ctcggccacc tcgaccactt cccactgcag
    10321 gtcatcgaag gtggagatgg ccgcctcgtc gtgacgctcg acggcatcgt cccaggcggc
    10381 ggacgcccgg cggtgcagct ccacgaggcg ggccagcgcc gcgtcgcggt cggtctcgcc
    10441 ccggggcggc tcgcgggtga ggcggacttc ttcggcgtcg aggtcggcga ggatcgcctg
    10501 gcggagcgat tcggggccct cccccgtggc ccgctggcgg cgctcgcgcc ggatgcgcgc
    10561 ctcgttgcgc aggcgctcga tctcattgag ggccggctgc gcctcctcct cgtcctccag
    10621 ccggttggcg gcccgctccc cgcgcccgat ccatgaaagc gccaggtcgt agtcgccggc
    10681 ccggatgagg gcccacgcgg cgttgagcgg cggccacgga tcgtccggca cggcgcgcag
    10741 caccttctcg tacatccgta gcccctcctc cttgcggccg gtcagcacga ggacctcggc
    10801 gtgtcgctcg cggcaggcat cgcgccgctc cggcgcgtcc cgctcgacgc gctccagcac
    10861 ctggagggcc tcaccgtact ccccgcgctc gcgcagcagg tcgtgcaggc gcaggcggat
    10921 gtcggcgtac gccagggccg ggtggccgcc ctccccgcgg tcgagccgcc tcaggagcgc
    10981 cattgcctcg ccggtccagc cctgctcctc gagcgactgc gccgccgccc acaggaggta
    11041 gtcgccggcc cgggcgagat cgagtgcgag gatctcccgc gcctccagat cgtcgaggat
    11101 ctgcatgtgc cgcggatact cctccgcgag ccgcccctcc tgctccgcga aggtccactg
    11161 gtagcccggc tcgatctcgt cgcggaggga gtcgtcctcc tcgtcgtcgt ggtcgtggtc
    11221 atcgtcgtcg tggcggtcgt cgccggggtc gccctgggcg gcgccgaaat cggggtcgtc
    11281 gggggcgtca ggggcgtcgg gatcgtcacg ttcatcgtcc ggtggcgtca gtggattcac
    11341 agggtcgcct cgtagtcgtg ccacgccgcc tccagcgcgg cgaggtcgtt gccgaaaatc
    11401 tccttgaagg cggcgaggcc gccctgcccg cccgcctcgc gcgcagcgta ccgtgagaag
    11461 gcggcggcgc ggcgcccgcg gtcactgtgg cgcaggaagt ggacgaaggt ccaggcggcg
    11521 ccgtaggcgc gcgccgaccg cccccccgcc cacagttcgt cctccgggcc ggcggcgagc
    11581 agcggctcga gcggcatcgg cccttcgacg cgggtgaggc gcaggtgctc gcgcagctcg
    11641 gcgcgcggat cgaactgtac ccgggcgctg ccgatgacga tcgaggcgct cgcgcggatc
    11701 gcgcccgcgt cgaccttcag atccccgtcg aggcgcgaga agccgaaata gttcgccagc
    11761 ccctcctcga accacttgct cggctgggcg cgcggcgggt agacccgctc gtagtcgagc
    11821 atgtggatcg cctcgtgcac gacgatcggg atcggcacgt gccccccggc cgcctcgcac
    11881 ggaaacgcca ggatgcgcga tgccggcagg tactcgccca ccgccgtcgc cgcgccggcg
    11941 gggcgccggc cgcgccagag ctgctgcacg cggatgtggt cgctgcgccg tccgaaggcg
    12001 tagacgtaca ccggctggcg gaagagccgt agcgtcgtca catcggccca gaactcctgg
    12061 tgggcgcgca ccgccgcgtt caccacctgg ccgagcacgg cggtctcgtc ggcgttgagc
    12121 gcgtcggtgt ggatcaccag ctgccccagc tcgacacgcg actggcggat ccccatgaga
    12181 cgcgccgcct cgttcatgta gtcgcgccgg tgccgctccg ccatgaagtc ggcgacgtcg
    12241 gcgtcgatcg ccagccgcag cgcctccccc tccgacaccc cgcgctggtg cacgatctgg
    12301 tactgcacct cggggccgtt tcccggcggc aggatggcgt agtacacctc ccccgacttg
    12361 cgcatcacgt actgacgccc gtcggcggcg accgggtcgg tccaggagcc gacccgcgcc
    12421 tcgtgcagcg cctggcgcag cacaccgggg aggtcggcgg ccgccaccag gccccagtcg
    12481 gcgtcgaggg cgctctcggg ggagacgtcc tcgaccgggg cgccggcccg cagcctcttc
    12541 accccttcct gcgccggccc cgtctggtcg aacgccagag actgcaagtc gaacagggcg
    12601 cgatgcgccc aggcgcgcga ctcggggggg aggcccgtga aggtgatctc caccggcgcg
    12661 aagtagcgca ccggatgccc cccgaggctg gccgggcgga agcgccagcc gttcaccgcg
    12721 gcgttcgccg aggcgcgcat cccgagatcg gggtcggggc cgcgcagcgt ccgggcgcgg
    12781 cgcggctcgc cgcgggggga gacgtcgacg aggagctcga cgcgcgcctc gccaatgagc
    12841 cgcgcccgcc ccgcctcctc ggggaacgtc ggcggcgcct cgcgcaccgg cacgggcggg
    12901 acgaccgcat cgaggggacc ggccagaccc ggattgtcgg gaggggggag aagggcgtcc
    12961 ggccccttcg tccgccgggc cccgggcccc tcgatcaact cacgagggat ccgcagcagg
    13021 cgcgtgcgga tcgcctcgct cacgtccttc tcgtccgctt cgaagccggc cgccacctgc
    13081 cacacgccgg gggcctcgcg ggtggagagg atcccctcga gaagccgcgg cccgccgccg
    13141 cagttcacct cgtaccgggc ccgcagccct cccgcgaagc ggctgacgat ctcgatccgg
    13201 tcgatcgcga cgcacccctc cgcggccggg tgcgcccagt cgaggaaatc gcgcagcccc
    13261 gaggcgaaca gatcccccgg cggcgaggcg ccggcgcgga tcggatcctc ccgcgccgcg
    13321 gccgggacga acgtcgcggt gtcctgcccg gccgcggcgc gatccttcgc ggcgtgatcc
    13381 gggtacgcct tctcgccggc ccgcaccgga accggaaggg cgttctcccg cggcgggatc
    13441 gggcagttgt agacggtggt gtaggcgcaa agcgggttgt aggcccggtt gaaatccacg
    13501 tcgtacaggc cgtccttcgg cgcgccgaga tcgaggtagc ggccggtggc gtacgactcc
    13561 ttcccggtcg tcgcatcggt gaagaggatg gagagcgtct cgtccggggc cgcggtgaac
    13621 agcggctggt acgcctgcac cttcaaggtg cgcggtcccc ccgcgccgcc cggcgcggac
    13681 acctcgaacc agccggccct gcggtattcc ttcgtgcggc cgagcgacgt cgcgaccgac
    13741 acggtctcgg gatgctcgtg gcggtggagc ggcagccgga acgaccagcg cgcgtcgtac
    13801 gggaagaact ccggtgccgt gaagccgcct cccgttccgc gccgggcgtc gaacacgatg
    13861 acgatgccgc tccccccctg gacgtacggc cgcagcgcga acggccccgc ctcgacgcgc
    13921 gtctcctcgg ggacgatccg ctcggtctcc cggaacggct cgcccgcgag cctcaggtcg
    13981 tccttctccg cgaggcgctc cagttggaag cggaaggggg cgccctcctt ctccggcgcg
    14041 agcaccgtca tgcgcagcag gcggccgggg atcccgggag cgtcgagccg cagatcgacc
    14101 tccgagccag atccgagcag cgccgacgag gcgccgttct cctccttcgg ctccgggaaa
    14161 tcgaaccggc gcacaccgcg cagcggggag aacggatcgt tgcggaagta ggcgatctgc
    14221 gcctgtcgcg ccgcagcgac tgcgtccccc gtcgcgttgg cggcgaccgg agccgccgca
    14281 cccaccgccg cggccggcgg ggacgccgcg agcggcagga cgaggatcgc agcgacgacg
    14341 ccgcgggccc gggctttcaa cggccgacct cgcggtgccg gaagcagtcg acgatgtggt
    14401 cgttcaccat gccggtggcc tgcatgtgcg aataacagat ggtgggcccc acgaagttga
    14461 agccgcgccc cctgaggtcg cggctcatgg cgcgggactc gtcggtctcc gccgggacct
    14521 gcttcagcgt cctccagaca ttgcacttcg gacgcccgtc cacgaagcgc cagatgtagg
    14581 cgtcgaacga gccgaactcc ttctgcacct tcaggaaggc gcgggcgttg cccaccgccg
    14641 aggccacctt ggcccggttg cgcacgatcg cggggtcctt gaggagacgc gcgaccttgc
    14701 gggcgtcgaa gcgggccacc cgggcggggt cgaagccggc gaaggcggcg cggtacgccg
    14761 cgcgcttccg gaggatggtg atccaagaga ggccagcctg cgccccttcg agtaccagga
    14821 actcgaagag acgctggtcg tcgtgcaccg gcacccccca ctcctcgtcg tgataggcga
    14881 tgtagagggg gtcgttcccc gcccacccgc agcgcgtcgc catggacgcc cgcaaggata
    14941 gcccggtcgc gggggccgga tcgagaggca cgagacaggg cgccacgggt cgggagagcc
    15001 ggtgcgccaa tcagattgcc ggtaggagac cgtccttcgc ggtcgcttcg tcgatcggcg
    15061 tctcgcgcat ggtgcgcgtc acgaccgaga tcagctcggc ggggtcgatc ggtttggcga
    15121 tgtgtccctg atatccgacc cgcagcgcgc ggtcgcgatc ctcgacgcga gcgtaggcgc
    15181 tcagcgccag ggccgggatg gccatgttcc tccggcggac ctcgccaatg aaggcatagc
    15241 cgtcctggcc gggcatggcg atgtcgcaca gaacgatatc cggcgtcacc gaatccagca
    15301 gcctcagacc ctccgtggcc gaggccgccg tgacgacgct tgcgccgcac cgctgcaggt
    15361 gcagggcgag gaaccgccgt gcatcttcct gatcgtcgat gaccaggacg gtggccccga
    15421 ccagagcgga atccgtcctg gccggcgccg catcgacgcc ggccggactc tcggagtcgg
    15481 gccgggcgga cggcgcctcc tcgatggcga gcggcaggcg gatggtgaag gtcgcccctc
    15541 gtccaatccc gtcgctctcg acacgcacgc ttccgccgtg cgcccgcacc agctgcctga
    15601 cgatggcgag ccccaggccg agccccccgt actcgcgcgt gctggaggtg tcgccctgac
    15661 ggaacggctc gaagacctcg gtcaggaact cgtggggaat gccgatcccg gagtcggcga
    15721 cggcgatctc ggcgtggccc tccgcttgct ccacctggac acggacgaga ccaccgggcg
    15781 gtgtgaactt gattgcgttc gagagcagat tcccgaggac ctgccggagc cggagcgcat
    15841 caccgcggac cgtgccgacc gtgccggcgt ccgaccggac ctccacgccc ttttccgccg
    15901 ccaggggacc gatcgcctcg accgcctcca cgacgagatc ggacgtccgg acgagcaccg
    15961 gctcgagctt cacgttcccc ctcaggatcc ggttcatgtc gagcaagtcg gcgacgagct
    16021 cggcctgggt cctggcgttc cgggagatga ccgccacggc ttcccgcacg agcgacgggt
    16081 cgggatcgtc ccggtccagg agtctggccc acccgaggat gacgtggatc ggcgtgcgca
    16141 actcgtgcga gagagtcgcc aggaattcgt ccttgatgcg gttggcccgc tccgactcgt
    16201 ggtgcgcacg ccgctcgttg tcgagcagcc gctgtcgttc ctcctcgtac gctttgtgga
    16261 tggtgatgtc gttgccggct tcgaggacgg cgtcgggtcc agccgggcgc cggtccagga
    16321 cccacctcga cgagacgatg acctttcggc cgtccctggt cgtatggacg agttcgccgt
    16381 cccaccggcc ctcgcgacgg agcgcggcct cgatcgactc cagcggctcc gtggactccg
    16441 tccgcagcag agcgtgactc gaccgtccca gcgcttcctc ctgcgtccag ccgtagagct
    16501 cctgcgctcc gtggttccag gtttcgatgc gatcgcccgg cccgcgcacg aagacggcat
    16561 cgctggtgag ttccagcagg cgcgcctgtt cgcgcgtccg ccgcgcctgc cgctcgaccg
    16621 cgcgcgccgt ccaccagagc acggcgagga agaaggcgac ttcggagaac gtccggaggg
    16681 ccgcttgaaa tctcgggtcg tagagtcccg cctggaggcc aacgatcgcc aggaagccgc
    16741 acgcgatcga agcgatgatc acgagcggca cggcccgact caggatgatc cccgcctcgc
    16801 tctcctcgat gatccggcgc accggcggga actctgggag gctcagcaga agagccaggg
    16861 cgacggcgag tatgaaggtg ctgctctgca gggcgatgat cgtgagccga ggcaggagga
    16921 acagctcgtc cgcccggtag aggtacccga cgatcgacag gaacgagatg ccggcggcga
    16981 ggcacgccag tcccggaacg gccgcacggg cgcgggtccg cgtcgacgcg agccataggg
    17041 cggtgccaag gatcgtccac gaaaccgccc cgggaagccc cattctgccc ggaaccagga
    17101 ccccccgctg tccccactcc cgaccgaaca gcaggatgtc gtcgatgccg agttcgatcc
    17161 ccaccagaca ctggaagagg accgacgcgc cgatccccgc caccacggca ccgaggaacg
    17221 ccgcggctcg cgtgcacttg gaaaccagca ggaggatggc cgttccggag agcaacgcgg
    17281 ccagggcggc gttcggctgg atggacacgc cgttgccact ccagtctgtg agccgcggcc
    17341 agttgagcgg ccatcccgag aacgagacca agccgcccgc gcatgcgtag gcggccagtc
    17401 cggcagcgac ccgtcgcagt ccgcgcgcgc gcaccaattc gggtgcggaa agttcctcgt
    17461 cagcctccga catgcggcgg gactgatggt ccgtggatca ttggattttc ttcttgaatc
    17521 gcccgaatac tatcacggag cgcgagagtg cgcgggattg gcgtcgcggt caaagcagct
    17581 cgcgcagacg caggttcccg gcccggctca aggggaggcg ccggccgtcc ttgaggatcg
    17641 cgacccggct gtccttggcg tacagctcga tccgatcgag gcggtcgatg ttgaggatcc
    17701 aggagcggtg gatccggacg aagcggcgcg ggtcgagggc cgcctcgagc tcgccgatcg
    17761 tctgctgctt gaggtgcgcc cggcctcccg cgtgcaccgc gatgtagtcg tcctgggcct
    17821 cgacgcagtc gacgtcgtcc atcgggatga cgtgcacgcg cgctccgtcg cgcaccagga
    17881 tgcggccgag cggctcgccg ggggggcggg ccgcggcgcc gatcgccgcg accggcatcg
    17941 gctcgcgccg gcgccggcgc tcgcgcgccc gctcgaccgc gcgcgccagc cgctccggat
    18001 cgaccggctt catgacgtag tccaccgcgt tcgcgtcgaa cgcctggacg gcccggtcgt
    18061 cgtgggccgt cacgaagacc acggcgatgc cgggatcgag caggctcagc acctcgaagc
    18121 cgtcgaggcg gggcatgcgg atgtcgagga gcaccaggtc cggcttcagc tccgccgccg
    18181 ccttcaccgc ctcgaatccg tcggcgctct cgccgacgac gtcgatcccc tcgagcgcgg
    18241 cgagatcccg cctcagccca cggcgcgcca gcgcctcgtc gtccacgatg aggacgcgca
    18301 gcgtctcagc cggcaagcga ggtctccgtt gccgcgacgt agggcagggt caccgtgacc
    18361 acgaacgcct ccggcggggt cgcgacctcg aggcgggccc ggtcgccgta gaggctcctg
    18421 agccggccgc ggacgttctc gagccccagg ccggcgccgc gccggccgct tctctccggg
    18481 ttcctcgggt tgtcgatggt cacggcgatc tcccccgctc cgcgccgctc cgcgcgcagc
    18541 cgcagcgtcc ccccttcgat ccgctgggcc acgccgtggc gcaccgcatt ctccaccagc
    18601 ggctggagca ggaggggcgg gatgagcgcc gcctcgcacc ccgcgtccac ctcgcgcgcc
    18661 acctccagcc gggagccgaa gcgcaccttc tcgatcgcca ggtactcgtc cacgagggcg
    18721 atctcctccc cgagcgtcac gcgatcccgg ccgcccagcg ccaggctgcg ccgcaggaac
    18781 ccggcgagga gcaggcacat gcgccgggcc gcccccgcgt cctcggaggt cagcgtggcg
    18841 atcgagttga ggctgttgaa caggaagtgg ggatccacct gtgcccggag cgcccgcagc
    18901 tccgcctcgc gcgccagcac ccgcgcctcc atcccgcgca cctccgccgc ccgcgccgcc
    18961 tcgagcgcca ggatgaggta gtgcgcggcg gcggcgagca ggaacaccag cgccccgacg
    19021 accaccagca tcggcgccga gccgcggagg cggagcgccg ctccgccgta gcgcggcagc
    19081 gtctcgagca tccgcgccag gatcccgccg aggacgaccc atgcccccgc cgaagccagc
    19141 gccgcggtcg cctgggtcac cagcgcccgc gtcatcccgc tcgcccgcag gggcgccgac
    19201 tggcacgggt agcgcgcggc gaggacgatc gccccctcca cgagccccag cggcaggcca
    19261 agcagcagcg cctcctccca ccccgccccg ccgccgagcg ccaggatcgt cgccgccagc
    19321 cccccgagga gcagggcaat cgtcaggaag agcccgatcc tcacaggcgc cagcggatca
    19381 cgaagtagat gacatacagc c
//
LOCUS       DASRIQ010000011         8759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1566008, whole genome shotgun sequence.
ACCESSION   DASRIQ010000011 DASRIQ010000000
VERSION     DASRIQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566008"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFD06_00225"
     CDS             <1..851
                     /locus_tag="VFD06_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01988.1"
                     /translation="YGLRNPWRSAFDPSNGDLYIADVGQDCYEEVNVLSAAAQPGANL
                     GWRSMEGLHCFAPGPINCNPAPVVCSGVPTCGDPSLVLPVVEYPHAQGCSVTGGPLYR
                     GCQMPDWHGTYFYGDFCSGFVRSFRYAGGVLTDQADRTAAIDPGATLVNDLTSFGLDG
                     QGEMYVIDRDGTVRRIGPRFVDLEVSAPGDAAPLRLEPAAWTWGDLAFSSMRPVSFYR
                     VYRGTPNGTFSCRFTSAAPQWPGGDPENPPPGALFAYVVTAVSPTGEETRPGIAGTSF
                     LRDACP"
     gene            1020..2921
                     /locus_tag="VFD06_00230"
     CDS             1020..2921
                     /locus_tag="VFD06_00230"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZN01989.1"
                     /translation="MRGAVATRGMGACGVAFLIGIGTVAAAPPGEVQQLRVSLSSLLI
                     WKPVTGATDYNVYRGLVSWMRRGDGVECHGDEIAMNYFRSIQNPPPGEAFFYLVTAES
                     NTAGEGTAGAGTGGAARPRRGACDALVRHHVLGRAAFGEDEWTRARIAARGIQGYLDE
                     QLDPASIDESTNSELLTRRALFVPPDNVDDLQALDIVTAVYARRQLEQQMTLFWNNHF
                     NTNYLKTDEWFNFYDPLFPKKQKLSAAVLHDEALTRFRDLAFNGTFRDLVEASGLSPA
                     MIIYLDTVANIAGAPNENYARELLELHTMGVHGGYTQQDIVQLARVFTGWNVCKKDAA
                     VAADPLSACIPSNTYGTATEPPGVWAVNFRPSRHDTSQKTLFAGTPYQAVIPSTAGNA
                     VDGINDAQLAFDAVVAHPSTARFIAAKLLQRFVDEQPTTAMIDAVVTAWNNPANPRGV
                     GDLREVLRAVLSQAAFRDPARAGAKLKTPFEHVVSALRALRGRTDGQSVVSGYLSRMS
                     ETYYENPVPTGFSEIGGDWLDTNNLLERQNFGYKLTESVYPYFGADVIGLLNANGIST
                     AAFPNNAPAIVDFLSGVLLGGDLTASERQRAIDFLSTDDSGLPAVYTDSRIRETAALL
                     MGFPPFLEQ"
     gene            2932..4332
                     /locus_tag="VFD06_00235"
     CDS             2932..4332
                     /locus_tag="VFD06_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN01990.1"
                     /translation="MSAPTDCDCSRRSFLRGAGLTLAGFGVTSLLPGPFLRHALAATA
                     GPFSNRRLIFIFLRGGNDGLNTVIPAGDPDYSAVTRPTLYIPNSSSLDLGNGFARLHP
                     AMSALLPLYTSGDLAIVHRVGLPNNSGSHFDDQRVWENGDPSRPPLLEGWLSRYIAEN
                     AVAAGVRLPALSVQANRPVLLRGGLPFVNISNPNEFDYVVPEPRRTKFEHSWEGTYAN
                     LTGLEPYRPLMSQTIVKLIDTLDEYRSWSQQTWDPKDPNNGFSLFPVSDATNPGFPPG
                     MFPPSSYSFFQSLKICALAMLESDGINNNGTRVAGCELGGFDSHILQTSSQPERLSVL
                     AYGLRSLQIVLSGAAIDPRGYPAVWNDTVVCTLSEFGRTTMENGSRGTDHAVASCLFV
                     AGGTVNGGVYNCDPSTWPAGVMFGKDGRYLSTRTDYRAIFWEILRDHMGAAPATVDQV
                     FPGYSGLGLTEVGLIA"
     gene            complement(4329..5333)
                     /locus_tag="VFD06_00240"
     CDS             complement(4329..5333)
                     /locus_tag="VFD06_00240"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZN01991.1"
                     /translation="MTIPRRQPGPGDVQIDILFCGVCHSDLHQVRNEWQSAMPTVYPC
                     VPGHEIVGRVAKTGAAVKGVKTGDLAAVGCMVDSCRVCDECKAGTEQYCKTLPTFTYN
                     FEDKHLGGVTYGGYSKSIVVDEAFVLRVPKGMDPAGAAPLLCAGITTYSPLRHWKVGK
                     GMKVGIVGLGGLGHMAVKFASAFGAEVTLFTTSPGKSEDARRLGAHDVVVTRDNEAEM
                     LKRAATYDYILNTVSAKHDLNVFLELLKKDATMTLLGAPQEPAALGVFSLLFKRRRLA
                     GSAIGGIRETQEMLDFCAERGIASDVETIRAAQINEAYARLLRSDVKYRFVIDMATLD
                     "
     gene            complement(5462..6034)
                     /locus_tag="VFD06_00245"
     CDS             complement(5462..6034)
                     /locus_tag="VFD06_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01992.1"
                     /translation="MEARRAVCSALTLCALLLIGCGEDDDPRSPGVGSPGVAGTSMGS
                     AQSLDGSWVITSDPENRNCGPLNALFETVTVMTIVQAGNDFNFTMVDNCGTSIPGGTG
                     SIDPGGTVRFGTEASRSLTSTCRVLLSEDWTGFAQIPTDEFNGSNVLSIVNSHQPGQD
                     DCGQSVPCTVSGSFIAERCPRSGCNVTCTP"
     gene            6216..6764
                     /locus_tag="VFD06_00250"
     CDS             6216..6764
                     /locus_tag="VFD06_00250"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HZN01993.1"
                     /translation="MRDRLNARAIAAAFTAVLFAAGGGLVAARAADRAAGMDGEILAQ
                     LVALNENEVHAAQLAEKKDAEGPVLSFAKMIREDRGRNINDTEKLGDKLNIDLKKSTA
                     VDSMRDEGERVLDRLDALKGDEFERSFLDEMVSGHRAALEKLDEWIREAQSPEVKSHL
                     QATRQRVAFHLKEAERLQGAVR"
     gene            complement(6801..>8759)
                     /locus_tag="VFD06_00255"
     CDS             complement(6801..>8759)
                     /locus_tag="VFD06_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01994.1"
                     /translation="FSGDRYALSPFEDLGMTLFTERNGVLSIETSRGEPLAEPARIVE
                     FARGAYPFEHLASGPFMAGDGAVIFDATVTGRDRGLYARAADGTLTAVAVGGGSAPGG
                     GPFYGPSFAFHSIATGGRVAFLGSTDWRGPREYAMQLFTGFVGGPFASIVVEGDSVDG
                     FDAPIAALLPPSRVHPDGTIVLPAVLQDGRSLLLRWNGTGLDVLARSGDVLPDGETIE
                     AIRTGPADAPFAPMLADDGAVFFGVLTASGHNVLYRFPPSGGLASTTRVLGDGTAVEG
                     NVLDPFLLRTMSVDAMGRLAFQAVTNPDAKVTTYLAEAASIPARVAGPGDGMPDGAVV
                     VEALPRLAATASGIVHETTLTHFSTQRVLWLAVPRAAIGNTPAFDQVPLNLGGTPAPD
                     GGTFRGGFSAGGVQPHLPISIARVGSDGERFAVSIEGTSQGAQILTLFDLRPDGVLPI
                     AAAGSDLVVECAGPLGTLVTLDGSGSADPAQGALSYAWSTPFGTATGPSPTVVLPLGA
                     WTVFLKVTNEQGLSSQDSVTVAVRDTIAPSVTVRALPDLLWPPDGRFVPIQLALTIQD
                     ACEPELPLQARLVAVTVDGSTRSAASRDVAGADYGTDDRTILVRAKRSGRGGGRAYTA
                     LYQVRDRSGNVGIGTATVTVPRRPPPGP"
BASE COUNT         1400 a         3045 c         2939 g         1375 t
ORIGIN      
        1 tctacgggct gcgcaatccc tggcgctcgg cgttcgaccc gtcgaacggc gatctctaca
       61 tcgccgatgt cgggcaggac tgttacgagg aggtcaacgt cctttccgcg gcggcgcagc
      121 cgggggccaa tctcggatgg cggtcgatgg agggactgca ctgcttcgcc ccgggcccga
      181 tcaactgcaa tcccgctccg gtcgtctgtt cgggggtgcc gacctgcggt gacccgtccc
      241 tcgtgctgcc cgtcgtggaa tacccccacg cgcaggggtg ctcggtcacc gggggaccgc
      301 tgtaccgcgg ctgccagatg ccggactggc atgggactta cttctacggc gatttctgct
      361 cgggattcgt ccgatcgttc cgctacgccg gcggcgtcct gaccgaccag gcggaccgca
      421 ccgcggccat cgatcccggc gcgacgctgg tgaacgatct cacgagcttc ggcctcgacg
      481 gtcaggggga gatgtacgtc atcgaccgcg acggcaccgt tcgccgcatc ggaccgcgtt
      541 tcgtggacct cgaggtctcc gcgccggggg acgccgcgcc gctgcggctc gagcctgccg
      601 cgtggacctg gggcgacctc gccttcagca gcatgcggcc ggtatcgttc taccgcgtct
      661 accgcggcac gccgaacggg accttctcgt gccggttcac ctcggccgcg ccgcaatggc
      721 ccggtggcga tccggagaac ccgccgcccg gcgctctctt cgcctacgtc gtcaccgccg
      781 tgagcccgac cggcgaggag acgcggccgg ggatcgccgg aacctcgttc ctgcgcgacg
      841 cctgcccctg acggaccggc cacggccggg tcggaggtgc tccaccgcac ccgacgaaaa
      901 agccgtttta gattggccac tcgaagaaaa attcgcatct ggcgattttt ctgcttcccg
      961 atccacgatt ttcggcgtat tagggtcccg gtccctccca gaccggaaag gagacgtcca
     1021 tgcgcggggc ggtcgctacg cggggcatgg gcgcttgcgg tgtcgccttc ctgatcggga
     1081 tcgggaccgt cgccgcggcg cctcccggag aggtccagca gctgcgggtc tctttgtcct
     1141 ccctgctcat ctggaagccc gtcaccgggg ccaccgatta caacgtctac cgcgggctcg
     1201 tctcatggat gcggcgcggg gacggcgtgg aatgccacgg cgacgagatc gccatgaact
     1261 acttcagaag cattcagaat cctccgcccg gcgaggcctt cttctacctg gtgaccgccg
     1321 aatcgaacac ggcgggcgag gggacggccg gtgccggcac cggcggcgcc gcgcgccccc
     1381 ggcgcggcgc ctgcgacgcc ctcgtgcgcc accacgtgct cggccgggcc gccttcggcg
     1441 aggacgaatg gacccgggcg cgcatcgccg cacgcgggat ccagggctac ctcgatgagc
     1501 agctcgaccc ggcctcgatc gacgagtcga cgaacagcga gctcctcacc cgccgcgccc
     1561 tcttcgtccc gcccgacaac gtcgacgacc ttcaggcgct cgacatcgtc actgccgtct
     1621 acgctcgccg gcagctcgaa cagcagatga cgctgttctg gaacaaccat ttcaacacca
     1681 actacctgaa gacggacgag tggttcaatt tctacgatcc gctcttcccc aagaagcaga
     1741 aactgtcggc ggccgtgctg cacgacgagg cgctgacccg atttcgcgac ctggcgttca
     1801 acggcacatt ccgcgacctc gtcgaggcga gcggactgag cccggcgatg atcatctatc
     1861 tcgacacggt cgccaacatc gccggggcgc cgaacgagaa ctacgcgcgc gagctgctcg
     1921 agctgcacac catgggggtc cacggcggct acacccagca ggacatcgtc cagctcgcca
     1981 gggtcttcac cggctggaac gtctgcaaga aggacgcggc ggtcgcggcc gatccgctct
     2041 ccgcctgcat ccccagcaac acctacggca ccgccaccga gccccccggc gtgtgggcgg
     2101 tcaatttccg cccgtcgcgg cacgacacct cgcagaagac cctcttcgcc ggaacgccgt
     2161 atcaggcggt gatcccctcg accgccggca acgcggtgga cggaatcaac gatgcgcagc
     2221 tggcgttcga cgcggtcgtc gcgcacccgt ccacggcgcg cttcatcgcg gcgaagctcc
     2281 tgcagcggtt cgtggacgag cagccgacga ccgcgatgat cgacgcggtc gtcaccgcct
     2341 ggaacaaccc ggccaatccg cgcggcgttg gcgacctgcg cgaggtgctc cgggcggtgc
     2401 tgtcgcaggc ggcgtttcgc gatccggcgc gcgccggcgc gaagctcaag acccccttcg
     2461 agcacgtggt ctcggccctg cgcgcgttgc gcggtcggac cgacgggcag tccgtcgtga
     2521 gcggttatct cagccgcatg tccgagacct actacgagaa cccggttccg accggcttct
     2581 cggagatcgg cggcgactgg ctggacacca acaatctcct cgagcggcag aatttcggct
     2641 acaagctgac ggagagcgtc tatccgtact tcggcgccga cgtcatcggt ctcctgaatg
     2701 ccaacgggat ctcgaccgcc gcgttcccga acaacgcacc ggcgatcgtc gacttcctgt
     2761 ccggcgtgct cctcggaggc gacctgaccg cctccgagcg gcagcgcgcc atcgacttcc
     2821 tcagcaccga cgacagcggg ctgccggccg tctacaccga cagtcggatc cgcgagaccg
     2881 ccgcgttgct gatgggcttt cctccgttcc tcgagcagtg aggggccatt gatgagcgca
     2941 cccacggact gcgactgctc ccgacgctcc ttcctgcgtg gcgcggggct gacgttggca
     3001 ggcttcggtg tgacgtcgct tctgccgggc ccgttcctgc gccatgccct ggcggcgacc
     3061 gccgggccct tctccaaccg ccggctgatc ttcatcttcc tgcgcggcgg caacgatggg
     3121 ctgaacaccg tcatcccggc gggagacccg gactacagcg cagtcacccg gccgacgctg
     3181 tacatcccga actcctccag cctcgatctc ggcaacggct tcgcgcggct gcacccggcg
     3241 atgtcggcgc tgctgccgct ctacacctcg ggcgacctgg cgatcgtcca ccgcgtcggc
     3301 cttcccaaca actccgggtc gcacttcgac gaccagcggg tgtgggagaa cggcgatccg
     3361 tcgcggccgc cgctcctgga ggggtggctg tcccgctaca tcgcggagaa cgccgtcgcc
     3421 gcgggggtgc ggctgccggc gctgtcggtg caggcgaacc ggccggtcct cctgcgtgga
     3481 ggtttgccgt tcgtcaacat ctccaatccg aacgagttcg actatgtcgt gccggaaccg
     3541 cggcgcacca agttcgagca cagctgggag ggaacatacg ccaacctgac cgggctcgag
     3601 ccgtaccggc cgctcatgtc gcagacgatc gtcaagctga tcgacacgct cgacgagtac
     3661 cgctcgtgga gccagcagac ctgggacccg aaggatccga acaatggatt ttccctgttc
     3721 cccgtgagcg atgcgaccaa ccccggcttc ccgccgggga tgttcccgcc ctcttcgtac
     3781 agcttcttcc agtcgctgaa gatctgtgcc ctggcgatgc tcgagtcgga cggaatcaac
     3841 aacaacggca cgcgcgtggc cgggtgcgag ctgggaggct tcgactcgca catcctgcag
     3901 acgtcctcgc aaccggagcg gctgtcggtg ctggcatacg gcctgcgctc gctgcagatc
     3961 gtcctgtcgg gggcggccat cgacccgcgc ggctaccccg ccgtctggaa cgacacggtc
     4021 gtctgcaccc tctccgagtt cggccgcacg acgatggaga acggcagccg cggcacggac
     4081 cacgccgtcg cctcctgtct cttcgtcgcc ggcggcacgg tgaacggcgg cgtctacaac
     4141 tgcgacccgt ccacctggcc ggccggcgtc atgttcggga aggacggccg ctacttgagc
     4201 acccgcaccg actaccgggc gatcttctgg gaaatcctgc gcgaccacat gggggccgcc
     4261 cccgcgacgg tcgaccaggt cttcccgggc tacagcggcc tcgggttgac cgaggtcggt
     4321 ctcatcgcct agtcgagcgt cgccatgtcg atgacgaagc ggtatttcac gtcgcttcgg
     4381 agcagccgcg cgtaggcctc gttgatctgc gcggcgcgga tggtctcgac gtcggaggcg
     4441 atcccccgct cggcgcagaa atcgagcatc tcctgcgtct cgcggatgcc gccgatcgcc
     4501 gagccggcga gccggcgtcg cttgaagagc aggctgaaga cgccgagggc ggccggctct
     4561 tgaggggcgc cgagaagcgt catggtcgcg tccttcttca acagctcgag gaagacgttg
     4621 agatcgtgct tcgccgagac cgtgttgagg atgtagtcgt aggtcgcggc ccgcttcagc
     4681 atctcggcct cgttgtcgcg cgtcacgacc acgtcgtggg cgccgagacg ccgcgcgtcc
     4741 tccgacttcc cgggagaggt ggtgaacagg gtcacctccg cgccgaaggc gctggcgaac
     4801 ttcaccgcca tgtgaccgag cccgccgagg ccgacgatgc cgaccttcat ccccttcccc
     4861 accttccagt gccgcagagg tgaataggtg gtgatcccgg cgcacaggag cggcgccgca
     4921 cccgccgggt ccatcccctt cggcacgcgc agcacgaacg cctcgtccac gacgatgctc
     4981 ttcgagtagc cgccgtacgt gaccccgccg aggtgcttgt cctcgaagtt gtaggtgaag
     5041 gtgggaaggg tcttgcagta ctgctcggtc cccgccttgc actcgtcgca gacccggcag
     5101 gaatccacca tgcagccgac ggcggcgagg tcgccggtct tgacaccttt gacggcggcg
     5161 ccggtcttcg ccacgcggcc gacgatctcg tgccccggga cgcagggata gacagtcggc
     5221 atggcactct gccactcgtt gcgcacctgg tgcaggtcgg aatggcagac gccgcagaag
     5281 aggatgtcga tctgcacgtc ccccgggccg ggctgccggc gcgggatggt catcggagcc
     5341 agcggggccg cggcgctcgt cgccccgcac gccctcgttt cgaaggttgc cttgtccacg
     5401 tcgtcctcct tccggattga ggttcgtgtg aggccagtct agtccggaag gaccgctttg
     5461 gtcagggcgt acaggtgacg ttgcatccgc tgcgagggca gcgctcggcg atgaacgagc
     5521 cgctgacggt gcagggcacc gactggccgc agtcatcctg ccccggctgg tgggaattga
     5581 cgatgctcag gacgttcgag ccgttgaact cgtcggtcgg aatctgcgcg aagccggtcc
     5641 agtcctccga caacagcacc cggcaggtgg aggtcaggga gcggctggcc tcggtgccga
     5701 agcgcacggt cccgccgggg tcgatgctgc cggtgcctcc cgggatcgag gtgccgcagt
     5761 tgtcgaccat cgtgaaattg aagtcgtttc cggcctgcac gatcgtcatg accgtgacgg
     5821 tctcgaacag cgcgttgagc ggcccgcagt tcctgttctc cgggtccgag gtgatgaccc
     5881 aggaaccgtc gagcgactgc gcgctcccca tggaggtgcc ggcgacgccc ggcgatccca
     5941 cgccggggga gcgggggtcg tcatcctcgc cgcagccgat cagcagcagc gcacacaggg
     6001 tcagggcgga gcagacggct cgtcgggctt ccattgcgat ttcctcccgc tgggtacgga
     6061 ttcttgagtg tggtcatcat acgccattcc ggcgccattc cggcggcggg ccgctcaggg
     6121 gaattccgga catccgggtc ggggtttctg ccccttgccc ccggcgcgcg gtcctctact
     6181 ctgggatcgg catatcccgg aggagacgac ccatgatgag agaccgattg aacgcccgag
     6241 ccatcgccgc cgccttcacc gccgtgctgt tcgcggcggg gggcggcctc gtcgcagccc
     6301 gcgccgccga ccgggcggcg ggtatggacg gcgagatcct cgcgcagctc gtcgccctca
     6361 acgagaacga ggtccacgcc gcccagctgg ccgagaagaa ggacgcggaa ggtccggtgc
     6421 tcagcttcgc caagatgatc cgcgaggatc gcggccggaa catcaacgac accgagaagc
     6481 tcggcgacaa gctgaacatc gacctcaaga agtccaccgc ggtcgacagc atgcgcgacg
     6541 agggggagcg ggtgctcgac cgtctcgacg ccctcaaggg ggacgagttc gagcgctcct
     6601 tcctcgacga gatggtgagc ggccaccgcg cggcgctcga gaagctggac gagtggatcc
     6661 gcgaggccca gagccccgag gtgaagtcgc acctgcaggc gacccgtcag cgggtcgcgt
     6721 tccacctcaa ggaagcggag cggctgcagg gcgcggtccg ctagcgggga tctttgggcg
     6781 atgcgacgac ccgaacccgg tcagggtccg ggcggcggcc gtcggggaac ggtgacggtc
     6841 gcggtcccga tgccgacgtt gcccgatcga tcgcgaacct ggtagagagc ggtgtaggcg
     6901 cgccccccgc cgcgaccgga ccgtttggcg cgcacgagga tcgtccggtc gtccgttcca
     6961 tagtccgcgc cggcgacgtc gcgactcgcg gcgctcctcg tcgaaccgtc gacggtgacc
     7021 gcgaccaggc gcgcctgcaa cggcaattcc ggttcacagg cgtcctgaat ggtcagcgcg
     7081 agctggatcg ggacgaatcg gccgtccggt ggccagagca ggtcaggaag cgcgcgcacg
     7141 gtcacggacg gggcgatcgt gtcccggacc gccacggtca cgctatcctg gctcgaaagt
     7201 ccctgctcgt tggtcacctt gaggaacacg gtccaggcac ccagcggaag gacgactgtg
     7261 gggctgggcc cggtcgccgt cccgaacgga gtgctccacg catagctcag cgcgccctgg
     7321 gccggatcgg ccgaaccgct cccgtcgagc gtgaccaggg tcccgagcgg ccccgcgcac
     7381 tcgacgacga ggtcagagcc agcggccgcg atcggcagta cgccatcagg ccgcaggtcg
     7441 aacagggtca ggatctgcgc cccctgcgag gtcccctcga tcgacacggc gaagcgctcc
     7501 ccatcggaac cgacccgggc gatggagatc ggaaggtgcg gctgcacacc cccggctgag
     7561 aatccacctc ggaacgttcc gccgtcgggg gccggcgtgc ctccgaggtt gagcggcacc
     7621 tggtcgaagg cgggagtatt tccgatcgcg gcgcgaggca ccgcgagcca caggacgcgc
     7681 tgggtggaga agtgcgtcaa tgtggtttcg tgcacgatgc cgcttgccgt cgccgcgagc
     7741 cgcggcagcg cctcgacgac gaccgccccg tccggcatcc catcccccgg gccagcgact
     7801 cgggcgggga tggaggctgc ctccgccaag taggtggtga cctttgcatc gggattcgtc
     7861 accgcctgga aggcgagccg tcccatcgca tccaccgaca tcgtcctcag caggaacgga
     7921 tcgaggacgt tcccctcgac cgccgttccg tcccccagca cacgcgtggt cgaggcgagg
     7981 ccgccggagg gagggaagcg atagagcacg ttgtggccgc tcgccgtcag gacaccgaag
     8041 aacaccgcgc catcgtccgc gagcatcggg gcgaaagggg cgtccgctgg tccggtccgg
     8101 atcgcctcga tcgtctcgcc gtcaggcagg acgtcgccgg atcgcgccag aacgtcgagt
     8161 ccggtcccgt tccaccgcag gaggagcgaa cgtccgtctt gcagaacggc cgggaggacg
     8221 atcgtgccat ccgggtggac gcgggagggg ggcaggagag cggcaatcgg cgcgtcgaat
     8281 ccatcgaccg aatccccttc gaccacgatc gacgcgaacg ggcctccgac gaagccggtg
     8341 aagagctgca tcgcatactc tcgcggtccg cgccagtcgg tacttccgag gaacgcgacc
     8401 cggccgccgg tggcgatcga atggaacgcg aacgaggggc cgtagaaggg gccgccgccc
     8461 ggcgcggacc cgcctccgac cgcgaccgcg gtgagggttc cgtccgcggc gcgggcataa
     8521 agaccacggt cgcgtccggt gacggtggcg tcgaagatca ccgctccgtc gcccgccatg
     8581 aacggaccgg aggccaggtg ctcgaatggg taggcgcccc tcgcgaattc gacgatgcgc
     8641 gccggctccg ccaggggctc acctcgtgaa gtctcgatgc tgagaacacc gttgcgctcg
     8701 gtgaacagag tcatcccgag gtcctcgaag ggggacaagg catagcgatc gccggagaa
//
LOCUS       DASRIQ010000012         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2869029, whole genome shotgun sequence.
ACCESSION   DASRIQ010000012 DASRIQ010000000
VERSION     DASRIQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2869029"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFD06_00260"
     CDS             complement(<1..959)
                     /locus_tag="VFD06_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN01995.1"
                     /translation="MTPPGALLVSLPFCGLPCPPARLTFERDRVTGAEGACSFCRQEA
                     TAPRATQARIAGKDASIDAALDRASELLSQSRRPFVYGLAASPVGTARLAARLAARID
                     AAVDVEGGDLLEPELGAIAATGQVTSTFGEMRASADLIVLWRIDPRATHPDLFPRGMK
                     ADPPAPAARRFVVVPPVSDHSGASIAPEAHDLVVPIPRGRDLQALQRLRGLLHRAPTR
                     TRDASLPVKELQAAAEAVASARRVAILWGGGIAADTPEGSAIAAGFALLTLDARPGVP
                     RIAAKALGPAGNVAGAMAGLLAATGFPRAIGFAAGRPRHDPDR"
     gene            1119..2162
                     /locus_tag="VFD06_00265"
     CDS             1119..2162
                     /locus_tag="VFD06_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZN01996.1"
                     /translation="MKSTTRILISLALTIGFLVLFLRSFDLEAAGRAVASASPGLLLL
                     AVILNLLAYVVRAWRWRHLLAPMRENLGLYNLTATTFIGFMITFLVPMRIGEIVRPVL
                     LARRERLPASAAIATIALERLFDMLTVMALFLVFSLSAHGRGVLDPSDAGAAQASAAL
                     LLRQGALLASVGVAVGLPIVILLVLFPGIVLRWLRRLNRGGQTSRLGRGIVLLEQFLA
                     GFGSLRRGRELGKIVASSLAMWLLIDLSTWFGLKAFGLPLQFFDTFLLMVALTVGISV
                     PTPGGVGPFEYFCQIALTDLWRVPVAVAGAVALTLHAVTLLPTIAVGLLFMWRDGVRP
                     AEVRNLATEGGTS"
     gene            2182..2640
                     /gene="nrdR"
                     /locus_tag="VFD06_00270"
     CDS             2182..2640
                     /gene="nrdR"
                     /locus_tag="VFD06_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZN01997.1"
                     /translation="MRCPFCTHLEDKVVDSREGKDGRVIRRRRECLKCAKRFTSYERI
                     DEIPLMVIKKDDRREPYDRNKILGGLRRACEKRPVSPTDLDSITDEIEALLQDSPERE
                     IATRAIGEKVMERLRTLDTVAYVRFASVYRQFQDVDQFMRELKEILEQRK"
     gene            2799..3248
                     /locus_tag="VFD06_00275"
     CDS             2799..3248
                     /locus_tag="VFD06_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZN01998.1"
                     /translation="MDSIGPFAEISRIQSEINRLFDNLIELRASKGEGGGEWLPSADV
                     YETERELVVKFEIPGVGAGDVTMSVEGNRLIVRGEKKRPENGHRGSRYHCMERGYGRF
                     KRVVHISSPVNTREAEAILREGVLEVTFPKVPNRRGGEMSIRITEKS"
     gene            3245..>4827
                     /gene="lon"
                     /locus_tag="VFD06_00280"
     CDS             3245..>4827
                     /gene="lon"
                     /locus_tag="VFD06_00280"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZN01999.1"
                     /translation="MTAGRDLPEGGEARVPKELPVLPLRDLVVYPFIIVPLSVSRERS
                     IKAVEKALSDNRMILLVAQKESETEEPGEKDLHTVGTVGVIMRMLKLPDSRIRVLVQG
                     VCRARVEGFQKKEPYLEAKIQRIHEPQAEGSLEVEALLRSTKKALERAVSLGKAISSE
                     VMVIAGNLEEPGRLADLVASNLDLKVEDAQGLLKVIDPVQRLRRTYELLNREIELLNM
                     QQEINTHAREEIDKSQREYFLRQQLKAIQSELGEGNDLSEEVEAFREKTKKARMPKKV
                     NDEVERQIGKLERMHPDSAETATLRNYLDWMVNLPWSAQTKDTLDLKKAQEILDTDHY
                     GLESIKERIIEYLAVRKLKKKMRGPILCFVGPPGVGKTSLGRSIARALGRKFVRISLG
                     GVRDEAEIRGHRRTYIGALPGQIIQGLRRAESKNPVFILDEIDKLGSDFRGDPASALL
                     EVLDPEQNSTFRDHYLDVPFDLSEVLFITTANVLDPVPPPLRDRMEVLELAGYTEEEK
                     LAIARDHLVSKQVENHGLTA"
BASE COUNT          789 a         1627 c         1703 g          708 t
ORIGIN      
        1 aagcgatccg gatcgtggcg gggcctgccc gccgcgaagc cgatggcgcg cgggaatccg
       61 gtcgccgcca ggaggccggc catcgctccc gcgacgttcc cggccgggcc gagcgccttc
      121 gccgcgatcc gcgggacgcc cggccgcgcg tcgagcgtca ggagcgcgaa ccccgccgcg
      181 atcgccgaac cctccggcgt atccgcggcg attcccccgc cccagagaat cgccacgcgt
      241 cgcgcggacg cgaccgcctc cgcggcggcc tgcaactcct tcaccgggag cgaagcgtcc
      301 cgggtgcggg tgggggcgcg gtggaggagg ccccgcaggc gctggagggc ctggagatcc
      361 cggccgcgtg gaatcggcac caccaggtcg tgagcctccg gcgcgatcga cgctccggag
      421 tggtcggaga ccggcggaac gacgacgaac cgccgcgccg cgggcgcagg aggatcggct
      481 ttcatgccgc gtgggaagag atccggatgc gtcgcgcgcg ggtcgatgcg ccagagcacg
      541 atgaggtcgg ccgaggcgcg catctccccg aaagtcgacg tcacctgccc ggtcgcagcg
      601 atcgccccca gctccggctc caacaggtcc cccccttcga cgtccaccgc cgcgtcgatc
      661 cgcgccgcca ggcgagccgc gagccgcgcc gtcccgaccg ggctcgccgc gaggccgtac
      721 acgaacggcc ggcgggattg ggaaagaagc tccgacgccc gatcgagcgc cgcgtcgatc
      781 gaggcgtcct tccccgcgat ccgcgcctgc gtcgcgcggg gtgcggtcgc ctcctgccgg
      841 cagaaggagc acgccccttc cgcgccggtg acgcgatccc gttcgaaggt cagccgcgcc
      901 ggggggcagg ggaggccgca gaacgggagg gaaacgagga gcgccccggg tggcgtcatg
      961 gcgcggcttg taccagagcc aaagacgcgc ggtcaaacgc tttgcaggat ccgccgtccg
     1021 aggggagcgg acccccccta cggaccaggg aggatcgccg gatgaaacgc cagaccacga
     1081 ccggcgactg atatagtcgg ccgcgttctg gggggcccgt gaagtcgacg acccgcatcc
     1141 tgatcagcct cgcgctgacg atcggcttcc tcgtcctgtt cctgcggtcg ttcgacctgg
     1201 aagcggctgg gcgggcggtc gcctccgcca gtcccggact gctgcttctg gcggtgatcc
     1261 tgaacctgct ggcgtacgtg gtgcgcgcct ggcgctggcg acacctcctg gcgccgatgc
     1321 gcgagaacct cgggctctac aacctcaccg ccacgacgtt catcgggttc atgatcacct
     1381 tcctcgtgcc gatgcgcatc ggggagatcg tccgcccggt gctcctggcg cggcgcgagc
     1441 gccttcccgc cagcgccgcc atcgccacca tcgccctcga gcggctgttc gacatgctga
     1501 cagtgatggc gctcttcctc gtgttctccc tgtcggcgca cggccggggc gtgctcgatc
     1561 cttccgacgc cggcgcggcc caggcgagcg cggcgctgct gctccggcag ggggcgctcc
     1621 tggcttcggt cggggtggcc gtcggactgc cgatcgtcat cctcctcgtg ctgtttcctg
     1681 gcatcgtctt gaggtggctg cgccggctca atcgcggcgg tcagacgagc cggctgggcc
     1741 ggggcatcgt gctgctggag cagttcctgg cgggcttcgg ctcgctgcga cgcggccggg
     1801 agctggggaa gatcgtggcc tcgtcgctcg ccatgtggct gctgatcgac ctgtccacgt
     1861 ggttcggatt gaaggcgttc gggctgccgc tccagttctt cgacaccttc ctgctcatgg
     1921 tggcgctcac ggtcggcatc tcggtcccga ccccgggcgg ggtcggaccc ttcgagtact
     1981 tctgccagat cgcgctcacc gatctctgga gggtgccggt ggcggtggcg ggggcggtgg
     2041 ccctgacgct ccatgccgtg acgctcctgc cgacgatcgc ggtcggcctc ctgttcatgt
     2101 ggcgggacgg cgtccggccg gccgaggtcc ggaacctcgc caccgagggg gggacgtcat
     2161 gaggggcggg aggggaaggc catgaggtgc ccgttttgca cccacctcga ggacaaggtg
     2221 gtggactcgc gcgaggggaa ggacgggcgg gtgatccgga ggcggcgcga gtgcctcaag
     2281 tgcgccaagc gcttcacctc ctacgagcgg atcgacgaga tcccgctcat ggtgatcaag
     2341 aaggacgacc ggcgcgagcc gtacgaccgg aacaagatcc tcggggggct ccggcgggcg
     2401 tgcgagaagc ggccggtctc ccccacggac ctcgactcga tcaccgacga gatcgaggcg
     2461 ctcctgcagg actcgccgga gcgggagatc gccacccgcg ccatcggcga gaaggtgatg
     2521 gagcggctgc ggacgctcga caccgtcgcc tacgtccggt tcgcgagcgt ctaccgccag
     2581 ttccaggacg tcgatcagtt catgcgcgag ctgaaggaaa tcctcgagca gcgcaagtag
     2641 ccggccagcc cttcgccggc gcgaaccgac ccggcgcgtc cggaatcttg gatgccgcgc
     2701 atcccaagac cgagcccctc gccgcggttg cgagggcgtg atcgcttcac tatattgacg
     2761 ccgcgccgga cgtaacgggt tccgccggga gggtcgggat ggacagcatc ggaccgttcg
     2821 ccgaaatcag ccgcatccag agcgaaatca accgcctgtt cgacaacctg atcgagctgc
     2881 gcgcctcgaa aggcgaaggc ggcggcgagt ggttgccgag cgccgacgtc tacgagaccg
     2941 agcgcgagct ggtggtgaag ttcgagatcc ccggtgtggg ggccggcgac gtcaccatga
     3001 gcgtcgaggg gaaccggctc atcgtgcgcg gcgagaagaa gcgccccgag aacggccacc
     3061 gcggcagccg ctaccactgc atggagcggg gctacggccg cttcaagcgc gtcgtgcaca
     3121 tcagctcccc cgtgaacacg cgcgaagcgg aggcgattct ccgcgaaggg gtgctggaag
     3181 tcaccttccc gaaggttccg aaccgtcgcg gcggcgagat gtcgatccgc atcacggaga
     3241 agtcatgacc gccggccgcg acctgccgga gggaggggag gcccgggtcc ccaaggagct
     3301 gccggtcctg ccgctccgcg acctcgtcgt ctacccgttc atcatcgtgc cgctgtcggt
     3361 cagccgggag cgctccatca aggcggtgga gaaggccctc tccgacaacc ggatgatcct
     3421 gctcgtggcg cagaaggaga gcgagaccga ggagccgggg gagaaggacc tgcacaccgt
     3481 cggcacggtc ggcgtcatca tgcggatgct caagctcccc gacagccgca tccgcgtgct
     3541 ggtgcagggg gtctgccggg cccgcgtcga agggttccag aagaaggagc cgtacctcga
     3601 ggcgaagatc cagcggatcc acgagccgca ggccgagggg tccctggaag tcgaagcgct
     3661 cctgcgctcg accaagaagg ccttggagcg ggcggtgtcg ctcggcaagg cgatctcgtc
     3721 cgaggtcatg gtcatcgccg gcaacctcga ggagccggga cgcctcgccg atctcgtcgc
     3781 cagcaacctc gacctcaagg tcgaggacgc gcagggcctc ctcaaggtca tcgacccggt
     3841 gcagcggctg cggcgcacct acgagctcct gaaccgcgaa atcgagctgc tcaacatgca
     3901 gcaggagatc aacacgcacg cgcgcgagga gatcgacaag tcgcagcgcg agtacttcct
     3961 gcggcagcag ctcaaggcga tccagtcgga gctcggcgag gggaacgatc tctccgagga
     4021 ggtcgaggcc ttccgggaga agacgaagaa ggcccggatg ccgaagaagg tgaacgacga
     4081 ggtggagcgg cagatcggca agctcgagcg catgcacccg gactccgccg agaccgcgac
     4141 cctgcgcaat tacctggact ggatggtgaa tctgccgtgg tccgcccaga ccaaggacac
     4201 attggatctg aagaaagccc aggagatcct cgacaccgat cactacggtc tcgagtcgat
     4261 caaggagcgg atcatcgagt acctcgcggt ccgcaagctg aagaagaaaa tgcgcggccc
     4321 gatcctctgc ttcgtcgggc cgcccggggt cggcaagacc tcgctcggtc gatccatcgc
     4381 gcgggcgctc ggccgcaagt tcgtccgcat ctcgctcggc ggcgtgcggg acgaggcgga
     4441 gatccgcggt catcgccgca cgtacatcgg cgcgttgcct ggacagatca tccagggttt
     4501 gcgccgggcc gagtcgaaga acccggtgtt tatcctcgat gagatcgaca agctgggttc
     4561 ggatttccgc ggcgacccgg cgtcggcgct gctcgaggtc ctcgatccgg aacagaactc
     4621 gacgttccgc gatcactacc tcgatgtgcc gttcgacctg tcggaggttc tgttcatcac
     4681 gaccgccaac gtgctcgacc cggtgccgcc gcctctgcgc gatcgcatgg aagtgctgga
     4741 gctcgccggc tacaccgagg aagagaagct ggcgatcgcg cgcgaccacc tggtgtcgaa
     4801 gcaggtcgag aatcacgggc tgacggc
//
LOCUS       DASRIQ010000013         4912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1827139, whole genome shotgun sequence.
ACCESSION   DASRIQ010000013 DASRIQ010000000
VERSION     DASRIQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827139"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VFD06_00285"
     CDS             complement(<1..1164)
                     /locus_tag="VFD06_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02000.1"
                     /translation="MYSGTRSTKSAPRPEPRRRPQGVRPRPQSRPPLLRRIGLAVLAA
                     AVAPAVVTAPAQAAQDERWLLQGLADFAWFDTDPGSRLLSVDGGDGTGSGALRLWAAG
                     ELAPGFQAFVMGAYENRPPLEGEEREPDTDLEQGYLRYTAPGRARLMVEAGRMVTPVG
                     DFSPRYLPDQNPLVGAPSDYSVTYPEGVKVVGWVKRFDMMAAAVNRPLNADWYALPSG
                     KAMRPMLSAGYTPVTGLRFGVFATEGPYLGEDAASSLEAGAEWQDFNQAVTGFEVRYG
                     IAHFEIHGQYASSRYEVPGHDRDSRGRAWYLEPRYTFSPRFFAAVRIENNDYPYIRPI
                     YGTTWIGGNSQVADIEAGVGVRFLPDLTLKVSYRRDKWTVDESLRPILPDGHAF"
     gene            complement(1121..3466)
                     /locus_tag="VFD06_00290"
     CDS             complement(1121..3466)
                     /locus_tag="VFD06_00290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HZN02001.1"
                     /translation="MTERLRRALSLRTQLLLFSGLLTVAVVGVMFALLGVTVRNQTRR
                     LLTATLIQHQQRMAAIQRSRTAELVRASVLLTESPTLRAALETYETERNGSGAGRGDL
                     LETIQHEVTRVASGLDRDLVVMTDLRGGVLAARARDPADVASATAGLRRAATGCAGVT
                     GEGLGEGARVPARSELVSFDGRLYQVGCVPILLGGDVIGGLVLGDRIDQAWVDRVRDD
                     LGADALVLSDGRVVASSRPGTPEELPAGTGGPAGAQDGSGHEGTLLTLADEELVAVSV
                     PLAGEGRGSNARLVLMHSLTKALAPTDRSLRQVLLLYGVLALVLAVAAATIVARSVLR
                     PLDRFVAFLRQVTETRDRGLRFDEPGGPEIQTLATSYNHLMAALLEHERRLLLQAREE
                     LDRMERLKESEKLAALGRMLSGAAHEINNPLAGVLGNIDLLLGDASVPVEARRRLETV
                     RREGQRIVGLVRNLLKTVHRDDGHRSLVDLNQVARDSAALRRHDFQTAGILLEVDLPG
                     QGTPVLGSELELQQVFLNVLNNAFDALTDARVENGRLAIQVRRDGGDAVVEFADNGPG
                     LKEPEKVFEHFYTTKPVGRGTGLGLSITSAIVRNHGGRIEAANRPAGGACFTLRLPAQ
                     EMPEVQEPMPPRHAAGGATAAPGPRRPARLAASVLVVDDEPSVLELQIAILETYGAQV
                     TGARSGATARDLLRELSFDLVVTDLRMPGEVSGQDLYRWARVERPGLASRFVFVTGDT
                     MSESALAFLEATGRRCVQKPFSVEGYLDVLRDTLNEKRAAA"
     gene            complement(3459..4394)
                     /locus_tag="VFD06_00295"
     CDS             complement(3459..4394)
                     /locus_tag="VFD06_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZN02002.1"
                     /translation="MPIHRREVRASGRVRLAAGAMVAAAIAAAAPPVPAAVRLVPPAA
                     HASSRQGQGDPESAECKDGAPGTPGCPAEMPARLDEWPARGARPSAGRLEGTVNLGER
                     LKQKRMRFALYPQDTQQALAGTRRASARGERDDEYRNVVVYLESTPALSAAARAGAAP
                     ASPSGPLSIRQEGLAFVPHVLPVLRGTTVEFPNTDPVFHNVFSLARAATFDLGRYPRG
                     DARSVRFDAPGTVKVFCHIHSDMSAVVLVLDNPFFVVPDSEGRFRLEGLPPGEYQAAA
                     WHERARLARRKVRIETGRATTVTFDIPLSEDGAGD"
     gene            complement(4541..>4912)
                     /locus_tag="VFD06_00300"
     CDS             complement(4541..>4912)
                     /locus_tag="VFD06_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN02003.1"
                     /translation="EEEDMAGYLALLYDDPAQFERLKPEEMQAIIERYRAWRIRMEGR
                     GAVQSGYKLADGEGKVLRRRGGRTEVKDGPFGESKEVVGGYFVLRAASYEEALSILED
                     CPHLEFGAIEVRAHDPRVPRD"
BASE COUNT          673 a         1837 c         1736 g          666 t
ORIGIN      
        1 gaaggcgtga ccgtcgggga ggatcggcct gagcgactcg tccaccgtcc acttgtccct
       61 gcggtaggag accttgagcg tcaggtccgg caggaagcgg actccgacac cggcctcgat
      121 gtcggccacc tgcgagtttc cgccgatcca ggtcgtcccg tagatcggcc ggatgtacgg
      181 ataatcgttg ttctcgatcc ggacggcggc gaagaagcgc ggcgagaaag tgtagcgcgg
      241 ctcgaggtac caggcgcggc cccgcgagtc gcgatcgtgg ccggggacct cgtagcgcga
      301 ggacgcgtac tgcccgtgga tctcgaagtg ggcgatgccg tagcgcacct cgaagccggt
      361 caccgcctgg ttgaagtcct gccactccgc gccggcctcg aggctcgaag cggcgtcctc
      421 tccaaggtat ggcccctcgg tcgcgaacac gccgaagcgc aggccggtca ccggggtgta
      481 cccggccgag agcatcggac gcatcgcctt cccggacggc agcgcgtacc agtcggcgtt
      541 cagcggacgg ttcaccgccg ccgccatcat gtcgaagcgc ttcacccagc cgaccacctt
      601 gaccccttcc gggtaggtga cgctgtagtc ggacggcgcg ccgacgagcg ggttctggtc
      661 cggcaggtag cgcggtgaga aatcccccac cggcgtgacc atgcgccccg cctcgaccat
      721 gagccgcgcg cggcccgggg cggtgtagcg caggtatccc tgctcgagat cggtgtccgg
      781 ctcgcgctcc tccccctcga gggggggccg gttctcgtac gcccccatga cgaaggcctg
      841 gaatcccggc gcgagttctc ccgccgccca cagccgcagc gcgccgctgc cggtgccgtc
      901 gccgccgtcg acgctcagga ggcgcgagcc cgggtcggtg tcgaaccatg cgaaatcggc
      961 gagcccctgc aggagccagc gctcgtcctg cgcggcctgc gccggtgcgg tgacgaccgc
     1021 cggggcgacc gcagcggcga ggaccgcgag gccgatgcgg cgcaggagcg gcgggcgcga
     1081 ctgcggacgg gggcggaccc cctgcggccg ccggcgcggc tcaggccgcg gcgcgctttt
     1141 cgttgagcgt gtcccggagt acatcgaggt agccctccac cgagaaaggt ttctgcacgc
     1201 agcgccggcc ggtcgcctcg aggaaggcga gcgccgactc cgacatggtg tcgccggtca
     1261 cgaagacgaa gcgcgacgcg agaccggggc gctccacccg ggcccagcgg tacaggtcct
     1321 gcccgctcac ctccccgggc atgcgcaggt cggtcaccac gagatcgaac gaaagctccc
     1381 gcaggaggtc ccgggccgtc gccccgctgc gcgcgccggt cacctgcgcg ccgtaggtct
     1441 cgaggatggc gatctgcagc tccagcacgc tcggctcgtc gtccaccacg agcaccgagg
     1501 cggcgagcct ggccggccgc cgcggccccg gggccgcggt cgccccgccg gcggcgtgac
     1561 ggggcggcat cggctcctgc acctccggca tctcctgcgc cggcaggcgc agcgtgaagc
     1621 aggccccgcc cgcgggacgg ttcgccgcct cgatccggcc gccgtggttg cgcacgatgg
     1681 cgctcgtgat gctcagcccc agcccggtgc cgcggccgac cggcttggtc gtgtagaagt
     1741 gctcgaagac cttctccggc tccttgaggc cggggccgtt gtcggcgaac tcgacgaccg
     1801 cgtcgccgcc gtcccggcgg acctggatcg cgagacggcc gttctcgacg cgcgcgtcgg
     1861 tcagggcgtc gaaggcgttg ttgaggacgt tcagaaacac ttgctggagc tccagctcgc
     1921 tgccgaggac cggcgtcccc tggcccggga gatcgacctc gaggaggatc cccgcggtct
     1981 ggaagtcgtg gcgccggagc gccgcgctgt cgcgcgccac ctggttgagg tcgacgagcg
     2041 accggtgccc gtcgtcgcgg tgcacggtct tgaggagatt gcgcaccagg ccgacgatcc
     2101 gctgcccctc gcgccgcacc gtctccaggc ggcggcgcgc ctccaccggg accgacgcgt
     2161 cgcccaacaa caaatcaata ttcccgagca ccccggcgag cgggttgttg atctcgtgcg
     2221 ccgcgccgga cagcatccgg ccgagcgccg ccagcttctc cgactccttg aggcgctcca
     2281 tccgatcgag ctcctcgcgc gcctgcagca gcaggcgccg ctcgtgctcc agcagggccg
     2341 ccatcaggtg gttgtaggag gtggcgagcg tctggatctc cggcccgccc ggctcgtcga
     2401 agcgcaggcc gcggtcgcgg gtctcggtca cctggcgcag gaaggccacg aagcgatcga
     2461 gcggccgcag cacggagcgg gcgacgatcg tcgccgcggc caccgccagc accagcgcca
     2521 gcacgccgta cagcagcagg acctggcgga gcgagcggtc ggtgggggcg agcgccttcg
     2581 tcagcgagtg catgagcacg aggcgcgcgt tgctcccgcg tccctcgccc gcaagcggca
     2641 ccgagaccgc caccagctcc tcgtcggcga gcgtcaggag cgtcccctcg tgtccgctcc
     2701 cgtcctgcgc tcccgccgga ccgccggtgc ctgccggcag ctcctcgggc gtccccggcc
     2761 ggctcgaggc gacgacacgg ccgtcgctga gcaccagcgc gtcggcgccg agatcgtccc
     2821 ggaccctgtc gacccacgcc tggtcgatgc gatccccgag cacgagcccc ccgatgacgt
     2881 cgccgccgag caggatcggc acgcacccga cctggtacag ccgcccgtcg aacgacacca
     2941 gctccgagcg cgccggcacg cgggcccctt cgccgagccc ctcgccggtg acgccggcgc
     3001 agccggtcgc cgcccgccgc agcccggcgg tggcgctggc gacgtcggcc ggatccctgg
     3061 cacgcgccgc caggaccccg ccgcggaggt cggtcatcac caccaggtcg cgatcgagcc
     3121 cgctcgccac gcgggtcacc tcgtgctgga tcgtctccag gaggtcgccg cggccggcgc
     3181 ccgatccgtt ccgctcggtc tcgtaggtct cgagggccgc ccgcagcgtc gggctctcgg
     3241 tcaggagcac cgaggcgcgc accagctcgg cggtgcgcga ccgctggatc gccgccatgc
     3301 gctgctggtg ctggatcagg gtcgcggtca gcaggcggcg ggtctgattg cgcaccgtga
     3361 cgccgagcag cgcgaacatc acgccgacga cggcgacggt gagcagcccg ctgaagagca
     3421 gcagctgcgt gcgcaggctc agcgcgcggc gcagccgttc agtcacccgc gccgtcctcg
     3481 ctgagcggga tgtcgaaggt caccgtcgtg gcgcgtcccg tctcgatcct caccttccga
     3541 cgcgccaggc gggcgcgctc gtgccacgcg gcggcctggt actccccggg cggcagaccc
     3601 tcgaggcgga agcgcccctc gctgtccggg accacgaaga acgggttgtc gaggaccagc
     3661 acgacggcgc tcatgtcgga gtggatgtgg cagaacacct tgacggtgcc gggggcgtcg
     3721 aagcgcaccg aacgggcgtc gccgcgcggg tagcggccga gatcgaacgt ggcggcgcgg
     3781 gcgagcgaga agacgttgtg gaacaccggg tcggtgttcg ggaactcgac cgtcgttccg
     3841 cgcaggaccg gcagcacgtg cggcacgaac gccagccctt cctgccggat ggagagcggc
     3901 ccggacggcg acgccggcgc cgcgcccgcg cgtgccgcgg ccgaaagcgc cggggtcgat
     3961 tcgaggtaga cgacgacgtt gcggtactcg tcgtcgcgct caccgcgggc ggacgccctg
     4021 cgggtcccgg cgagcgcctg ctgcgtatct tgaggataga gcgcgaagcg catccgcttc
     4081 tgcttcagcc gctcgccgag gttgaccgtc ccctcgagcc gtcccgccga cggccgcgcg
     4141 ccgcgcgccg gccactcgtc gagtcgcgcc ggcatctcgg ccgggcatcc gggcgtcccg
     4201 ggcgcgccgt ccttgcattc cgccgactcc ggatccccct gtccctggcg cgacgacgcg
     4261 tgcgccgcgg gaggcacgag acggacggcg gcggggaccg ggggcgccgc cgcggcgatc
     4321 gcggccgcca ccatcgcccc ggcggcgaga cggacccttc ccgaggcgcg gacttcccga
     4381 cgatggatcg gcatacgacc tccccgcgcg gcgcactccc ccgccaggtg gtcaatatag
     4441 gcaggccgat cggcgcttca aggacggccg cgtcccgatt acgaatcggt gatccatcct
     4501 cttttgatcc ggccactttc ggcccgatcg gccgcttcgc tcagtcccgc gggacgcgcg
     4561 gatcgtgggc gcgcacctcg atggcgccga actcgaggtg cgggcagtcc tcgaggatcg
     4621 agagcgcctc ctcgtacgag gcggcgcgca ggacgaagta gcccccgacg acctccttgc
     4681 tttcgccgaa cggcccgtcc ttcacctcgg tgcgtccccc cctgcggcgc agcaccttcc
     4741 cctcgccgtc ggcgagcttg tagccgctct gcaccgcccc gcgcccttcc atgcggatcc
     4801 gccacgcgcg gtagcgctcg atgatcgcct gcatctcctc cggcttcaga cgctcgaact
     4861 gggcgggatc gtcatagagc aacgccaggt aaccggccat gtcctcctcc tc
//
LOCUS       DASRIQ010000014         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_784917, whole genome shotgun sequence.
ACCESSION   DASRIQ010000014 DASRIQ010000000
VERSION     DASRIQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784917"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VFD06_00305"
     CDS             <1..1169
                     /locus_tag="VFD06_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZN02004.1"
                     /translation="PYFAVGQVGNVVVLENTVEGSDAKHTVAVNYDHLPRGQYTINAD
                     PKDLKAPKLDKKTPPDLYQARNAVNLARAAGADQFAVNSFRKASDLLAQAERNQNDKK
                     GNKKAVAPLSRHAVQQAEDSRQLAIRVQADEQVVAEKRALKETAAAEKRSVEERAAAD
                     RRSMQEQAAADRRSWQEQTAAERQQAAAERQAMAERAASEKQAMAAQIAAAQTEAANE
                     ASRRAAAQQEAEQARFAVAQSEQEQTRLRAQLREQLNAVMKTTDSARGLIVSMTGLLF
                     ETGKSTLLPNVRENLARVAGILLGHPGLEIEVEGHTDSVGSDAMNQTLSENRAEAVRQ
                     YLLSQGVSKDTIVSRGFGETRPLATNDTSDGKAQNRRVEIVVSGESIGADVPAR"
     gene            complement(1405..2187)
                     /locus_tag="VFD06_00310"
     CDS             complement(1405..2187)
                     /locus_tag="VFD06_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN02005.1"
                     /translation="MFLVNAGRRRTRSAIFGILLAALGSPALAQGEIPDKFKNLKVLP
                     KDIKQGELVGVMRTFAGALGVRCAYCHVGEDGKPLSTFDFASDEKTPKRAARDMLRMV
                     DAINDRYLAKADLGRTERLTVNCVTCHHGLTRPEQLQTILTRITKEKGIDAALEEYRT
                     LRKKYYGGAQYDFGGPPLNMVAERLMRDGKIDEAIKAAELNAEEHPDISHAHSFLGDL
                     YAKKGDKARAIEAMQRALAADTANDYARRRLDALQKEEPAKP"
     gene            2389..3984
                     /locus_tag="VFD06_00315"
     CDS             2389..3984
                     /locus_tag="VFD06_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN02006.1"
                     /translation="MILLIAGLVLAVVLAWQAADAAGSHRAATAAVLRDYAGLAGAEF
                     IRRSTALVGYEGCFRLVTAVRQATPPVPRDAVASRSEVAARLDSETRRALDLASRYLR
                     FDGVSGRLTATDPLPEEVSSEMARALRESAGRIAAGRGAFSVIPVNVAGSSRAFVMVP
                     IGGSAEGAPADLAGFELDLDALAPWFRQVLDRGPLLPDSIGHGKVGNHHLSLEVVDIA
                     GRTRFRAGAFRYGGFTVDLPFGDAYEGMFEGMHLRASLDEAAAPALVIGGLPRSRLPI
                     LLALLLLSAGLVAAGLVLLRRDRAVAALRTEFVSRVSHELRTPLTQIRLFAETLLLDR
                     VRDDEERRRALQVIDDEARRLGHLVENVLRFARGERGALHLDLRSRDLGPLIEETIEG
                     FRPMADRAGAAVDSRLAGDLTARLDADALRQILLNLLDNAVKYGPEGQRIRVEAERLD
                     GACRIRVEDQGPGIPPGERERIWESFRRLRREENAAIAGTGIGLSVVRELTLLQGGRC
                     WVEDGSDGGARFVVELPAGGTDS"
     gene            3981..4676
                     /locus_tag="VFD06_00320"
     CDS             3981..4676
                     /locus_tag="VFD06_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN02007.1"
                     /translation="MSRILIVEDNRSLGDGLRNNLEIEGHEVEVARDGKAGLDRARAL
                     VPDLVILDLMLPKMDGFRVLQALRQEGHQFPVLILSARGEEADKVRGLRLGAEDYLTK
                     PFGLLELLARVDGLLRRGSRAAGGREPGPGGTDRFGSVVVDHDRRTVTRDGRPVDLAP
                     REFDLLVALIRRRGAVASRSDLLREVWGYSSGAVSRTVDTHVAELRRKLEEDPGEPRH
                     ILTVRKVGYRHQG"
     gene            complement(4799..5524)
                     /locus_tag="VFD06_00325"
     CDS             complement(4799..5524)
                     /locus_tag="VFD06_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02008.1"
                     /translation="MSRRTIGLIALLSFCLALPAAAQEPAGTAAGTKDHATAVKESLQ
                     KSMAALRQYQWVETTAVSMKGEEKSRDQKNCYYGADGKVQKTPVAAAPASAKDDKRSR
                     GVKARVVENKTEEISDAVKEAIGLVKQYVPPDPARIQAAKDAGRVSVTPPDPAGQVRL
                     VIKDYLKPGDSLTLDANAATDRISGVTVATFTEKAKDAVGLKVAFGAFADGTVYPSNI
                     QLDVAAQNLAIAIENSGYKKVGG"
     gene            5662..>6093
                     /locus_tag="VFD06_00330"
     CDS             5662..>6093
                     /locus_tag="VFD06_00330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN02009.1"
                     /translation="MFIPLGDDNRDRRIAPLVNIALIAANVLVFVFLQGFGSNVRFTY
                     SLSMVPAEILSGRDVVTEAREVTDPATGEQFEVPGLQPTPWSVYLTLLTSMFMHGGLA
                     HLLGNMLFLWIFGDNIENGMGHVRYLVFYLLTGVLASLAHLA"
BASE COUNT          879 a         2098 c         2136 g          980 t
ORIGIN      
        1 agccgtattt cgccgtgggc caggtgggca acgtcgtcgt tctcgaaaac accgtggagg
       61 ggagcgacgc gaaacacacg gtggcggtga actacgacca tctgccgcgg ggtcagtaca
      121 ccatcaacgc agatcctaaa gacctcaagg ctccgaagct ggacaagaag accccgccgg
      181 atctctacca ggcgcgaaat gccgtgaatc tcgcccgggc cgccggcgcc gaccagttcg
      241 ccgtcaattc cttcaggaag gcgtcggatc tgctggccca ggcggagcgc aaccagaacg
      301 acaagaaggg gaacaagaag gcggttgcac ctctctcccg ccacgccgtg cagcaggccg
      361 aggactcccg ccagctggcg attcgcgtcc aggctgacga gcaggtcgtt gcggagaagc
      421 gagcgctgaa ggaaacggcc gccgccgaga agcgcagtgt cgaggagagg gccgccgccg
      481 atcgacggtc catgcaggag caggctgccg ccgatcgcag gtcctggcag gagcagaccg
      541 ccgcagagag acagcaggcg gccgcggaga ggcaggccat ggcggagagg gcggcttcgg
      601 agaagcaggc gatggcggcg cagatcgccg cggcgcagac ggaagccgcc aacgaggcga
      661 gccgccgcgc ggcggcgcaa caggaggccg agcaggcgcg gttcgccgtg gcgcaatccg
      721 agcaggagca gacccggctt cgcgcgcagc tccgggaaca gctcaacgct gtcatgaaga
      781 cgaccgactc ggcgcgcggg ctcatcgtga gcatgacggg ccttctcttc gaaaccggca
      841 aatcgactct tctcccgaat gtccgcgaga atctcgcccg cgtcgcgggg attctgctcg
      901 gccatccggg gctggagatc gaggtcgaag ggcacaccga cagcgtcggc tcggacgcca
      961 tgaaccagac cctctccgag aaccgcgccg aggcggtccg acagtacctg ctgtcgcagg
     1021 gggtttcgaa agacaccatc gtctcgcgcg gcttcgggga aacgaggccg ctcgccacga
     1081 acgacacctc ggacggcaag gcgcagaacc ggcgggtgga aatcgtcgtc tccggggaat
     1141 cgatcggagc cgacgtgccg gcccgctgag cctgattccg cgacgggctt ttcagcacga
     1201 gggcaaggcg agcgtccaga gggcggcgaa cagccggcca cgcgcgcgcc gggcgccgcg
     1261 gaccagcgcc gccgcccagg gcgacggccc cgtttcccgc cagcaggtgc agctcgacgg
     1321 cgagatggcc ggaccgcggc tgtcaacagc tcgaagcgcg cgcccggtgc cgaggtcctg
     1381 ggagccgacc gacggggcgg ggatctaggg cttcgccggc tcctccttct gcagcgcgtc
     1441 gagacggcgc cgggcgtagt cgttggcggt gtcggcggcg agcgcgcgct gcatcgcctc
     1501 gatggcccgg gccttgtccc ccttcttggc atacaggtcg ccgaggaatg aatgggcgtg
     1561 cgagatgtcc gggtgctcct ccgcgttgag ctcggcggcc ttgatggcct cgtcgatctt
     1621 gccgtcgcgc atcaggcgct ccgccaccat attgagggga gggccgccga agtcgtactg
     1681 cgccccgccg tagtacttct tccgcagcgt ccggtactcc tcgagcgccg cgtcgatccc
     1741 tttctccttg gtgatacgcg tcaggatcgt ctggagctgc tccgggcgcg tgagcccgtg
     1801 atggcaggtg acgcagttga ccgtaagcct ctcggtacgg ccgaggtccg ccttggcgag
     1861 gtacctgtcg ttgatggcgt cgaccatgcg cagcatgtcg cgcgccgccc gcttcggcgt
     1921 cttctcgtcg ctggcgaaat cgaaggtcga cagcggcttc ccatcctcgc cgacgtggca
     1981 gtaggcgcaa cggaccccga gtgccccggc gaaggtccgc atcacgccga ccagctcccc
     2041 ctgcttgatg tccttgggca ggaccttgag gttcttgaac ttgtcgggga tctccccctg
     2101 cgccagggcc ggggagccca gcgccgccag caggattccg aagatcgcgc tccgcgtccg
     2161 cctgcgtccc gcgttcacca agaacatgtc gagttcctcc ccgcgcccgt tgcggcggtc
     2221 cgagacgtta gcagtggcag gcccggggag aggcaagggt tcggtccggg aattgtcagc
     2281 gattcgtcaa ctccttcggc cgccgccctg acggtccggg ggctgcgcta gagtcggcgc
     2341 atgggtctgg cgtcgaggcg ccgcgacgcg ttccgcgggt cgggattggt gattctgctg
     2401 atcgccgggc tcgtcctggc ggtcgtgctc gcgtggcagg cggccgatgc cgccggctcg
     2461 catcgggccg cgaccgcggc ggtcctgcgc gattacgccg ggctcgccgg cgcggagttc
     2521 atccggcgtt ccaccgcgct cgtggggtac gaagggtgct tccggctggt caccgcggtc
     2581 cggcaggcga ccccgccggt cccgcgcgac gcggtcgcct cccgaagcga ggtcgccgcg
     2641 cgcctcgact ccgagacgcg ccgggcgctc gatctcgcgt caaggtatct gcgcttcgac
     2701 ggcgtctccg ggcggctgac ggcgaccgat ccgctgcccg aggaggtgtc ttccgagatg
     2761 gcccgtgccc tgcgcgagtc cgcgggaagg atcgccgcgg ggcggggggc gttcagcgtc
     2821 atcccggtga acgtcgccgg gtcgtcgcgc gccttcgtga tggtgccgat cggcggctcg
     2881 gccgagggcg ctcccgcgga tctcgcgggg ttcgagctgg acctggatgc cctcgccccc
     2941 tggttcaggc aggtgctgga ccgaggcccg ctgctccccg attcgatcgg tcacgggaag
     3001 gtcggcaacc accacctttc cctcgaagtg gtcgacatcg cgggacggac ccgcttccgc
     3061 gccggagcgt ttcgttatgg gggattcacc gtggacctcc cgttcgggga cgcctacgaa
     3121 gggatgttcg aggggatgca cctgcgcgcc tcgctggacg aggcggcggc gccggccctc
     3181 gtcatcgggg gcctgccgcg ctcccgcctg ccgatcctgc tcgctctgct gcttctgtcg
     3241 gcgggactgg tggcggcggg gctcgtgctg ctgcggcggg atcgggcggt cgcggcgctt
     3301 cgaaccgagt tcgtgtcccg cgtgtcgcac gagctgcgca ccccgctgac gcagatccgc
     3361 ctgttcgccg agaccctcct tctggaccgg gtccgcgacg acgaggagcg gcgccgggcc
     3421 cttcaggtga tcgacgacga ggcgcgccgg ctcggccacc tcgtggagaa cgtcctgcgc
     3481 ttcgcccggg gcgagcgcgg agcgctgcat ctcgatctca ggagccgcga tctcggcccg
     3541 ctgatcgaag agaccatcga gggcttccgg ccgatggccg atcgcgccgg ggccgccgtc
     3601 gacagccggc tcgcaggcga cctcacggcg cgtctcgacg ccgacgccct gcggcagatc
     3661 ctcctcaacc tgctcgacaa cgccgtcaag tacgggccgg agggacagcg catccgtgtc
     3721 gaggcggagc gcttggacgg cgcgtgccgc atccgcgtgg aggatcaggg cccggggatc
     3781 cccccgggcg agcgcgagcg catctgggag tcgttccggc gcctgcgccg cgaggagaac
     3841 gccgccatcg ccggcaccgg catcggactc tccgtcgtcc gcgagctcac gctgctgcag
     3901 gggggacgct gctgggtgga ggacgggtcg gacggcggcg cgcgcttcgt cgtcgagctt
     3961 cccgccggcg ggacggactc atgagccgca tcctgatcgt cgaggacaac cgcagcctcg
     4021 gcgacggcct gcgcaacaac ctcgagatcg aagggcacga ggtcgaggtg gcgcgcgacg
     4081 gcaaggccgg cctcgatcgg gcgcgcgccc tggtccccga tctcgtcatc ctcgacctga
     4141 tgctcccgaa gatggacggc ttccgcgtcc tgcaggcgct gcgccaggaa gggcaccagt
     4201 tcccggtgct catcctgagc gcgcgcgggg aggaggcgga caaggtgcgc gggctgcggc
     4261 tcggggcgga ggactacctg acgaagccgt tcggcctcct cgagctgctc gcccgggtgg
     4321 acggcctgct gcgccgcggc tcccgcgccg ccggggggcg cgagcccggg ccgggcggga
     4381 cggatcgctt cggttcggtc gtggtcgatc acgaccgccg cacggtgacg cgggacggcc
     4441 gaccggtcga tctcgccccg cgcgagttcg acctgctcgt cgccctcatc cggcgccgcg
     4501 gcgccgtcgc gtcccgctcc gacctcctgc gcgaggtctg ggggtactcg tcgggagcgg
     4561 tgtcgcgcac cgtcgacacc catgtcgccg agctgcgccg caagctggag gaggatccgg
     4621 gcgagccgcg ccacatcctg acggtccgca aggtcgggta ccgtcatcag ggatgaacgg
     4681 aagggacgcc ttccgccaga atcctccgcc gcttgtcccc tcgtcgtccg gcgcatgtcc
     4741 ggattttcct tcgatggcgc cagccccgcc gcgccggcgg ctactcgacg ctgcagcctc
     4801 agccgccgac tttcttgtag ccggagttct cgatcgcgat cgcgaggttc tgcgcggcca
     4861 cgtccaactg gatgttcgac gggtagaccg tgccgtcggc gaaggcgccg aacgccacct
     4921 tcaacccgac cgcgtccttg gccttctcgg tgaacgtggc gaccgtcacc cccgagatgc
     4981 gatcggtggc ggcgttcgcg tcgagcgtga gggagtctcc cggcttgagg tagtccttga
     5041 tcaccagccg gacctgtccg gccggatcgg gcggcgtcac cgagacgcgc cccgcgtcct
     5101 tggccgcctg gatgcgggcg gggtcgggcg gaacgtactg cttcacgagg ccgatcgcct
     5161 ccttcaccgc gtcggagatc tcctccgtct tgttctcgac caccctcgcc ttgacccccc
     5221 gcgacctctt gtcgtccttc gccgaagccg gcgccgccgc gaccggggtc ttctgcacct
     5281 tgccgtcggc accgtagtag cagttcttct ggtcgcgcga tttctcctca cccttcatgg
     5341 agaccgcggt ggtctcgacc cactggtact ggcgcagggc cgccatgctc ttctggagcg
     5401 attccttcac ggcggtcgcg tggtctttcg tcccggcggc cgtgcccgcg ggctcctgcg
     5461 ccgcggccgg cagggcgaga cagaacgaga gcagcgcgat gagaccgatg gtgcgacggg
     5521 acatgaaagc ctcctcttct cgaaccgcga atcgcgatgg cgccgaccga ctggggctgt
     5581 acaccctccg cggcgctgcc tcccgattgt tgttgagatg aaccgagtgt actcgaaaac
     5641 cttccaagat atcatcccgc gatgttcatt cctctcggcg acgacaatcg cgatcgacgg
     5701 atcgcgccgc tggtcaacat cgcgttgatc gccgccaatg tgctggtctt cgtcttcctg
     5761 caaggattcg ggtcgaatgt ccgcttcacc tactccctgt ccatggtgcc ggcggaaatc
     5821 ctcagcggcc gggatgtggt caccgaggcc cgcgaggtaa ccgacccggc gacgggagag
     5881 cagttcgagg tgccgggact ccagccgaca ccgtggagcg tctacctgac gcttttgacc
     5941 tcgatgttca tgcacggcgg gttggcgcac ctgctcggca acatgctctt cctgtggatc
     6001 ttcggcgaca acatcgagaa cggcatgggg cacgtgcgct atctcgtctt ctatctcctg
     6061 accggggttc tggcgtcgtt ggcccacctg gct
//
LOCUS       DASRIQ010000015         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3130175, whole genome shotgun sequence.
ACCESSION   DASRIQ010000015 DASRIQ010000000
VERSION     DASRIQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3130175"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1849)
                     /locus_tag="VFD06_00335"
     CDS             complement(<1..1849)
                     /locus_tag="VFD06_00335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN02010.1"
                     /translation="MTSEARASARRAVPARRLRTIAAAAPLLALLACLAGVTWRRWPD
                     LLIDFGQQLYIPWRLAAGERLGTDVLYFHGPLSQHLNALLFRVFGVSFSTLIGANLLL
                     VVLLTWILARLVRRASDRLTGWVAAAAFLCLFAFAQYVMVGNYNFLAPYTHEATHGLL
                     FGAGMVLLIGRFLRRGCRRSAAAAGLLLGLALLTKVEAAVAALAVAAAAVFVAALRAE
                     DRGRRLRGLLPCAAAAVVPSLLFFLWFLTYLPPGEAARATAAGFLALGGGVTRFAFHR
                     HVLGIDDLPGNLLGLTRSAATFGFGVLMLVVTDRLLAPRARRPVLWGAAAGLAFLFVV
                     TAAGASIWVELPRALPLVAAVAIAASAWRLLRSGSVPRAERGRREALLLLAVWALAAT
                     LKGILNLHLYHYGFTLALPATMLALVLLLHEGPARMAARGGGSGVVFRFAALGIVAAA
                     LLFHVRWTMELAGLRTFPVGRGGDRIVAFAPEVAPGGPIAARAIDWIERTTPEGATLV
                     ALPEGIMLNYQTRRLSTVPFTNFVTAEAVLFGQDRLVAALDRRPPDFVAIVARKTEEL
                     GTGRFGFVPGYGKRVVDWVEAHYETAAQFGADPLVADDGGIRILKRRALA"
     gene            1929..3500
                     /locus_tag="VFD06_00340"
     CDS             1929..3500
                     /locus_tag="VFD06_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZN02011.1"
                     /translation="MRVGLDIGSSSAKALVVDAAGRARRAVRRTFPTRRDRGRVEHDG
                     ERVAAATLGALEDLAPSLPARAAVPLGFASQRSTFLFWSRDTGRPLTPAISWQDLRAG
                     GINRGVRRRAERTGIDIGQVTGLRPSAHYAAPKIAWALRRVPGLGRQVASGRALWGTL
                     TTFIVWRLTRGGVYAVDHANAQRTSLMDLASLAWEPRLFEIYGLEALLDAPALPALTP
                     TCLTTPIDVAIGGRRLALAAVTGDQQAALVGLRCRRRGDMAINYGSGAFVLRCVGGTP
                     LRRPGLLTTLVTSRRTLPASGRGGGLTASYALEGPVNAAATALDWVAARMHRRVHTAD
                     LDRLLGPWDPRARRAIHFLPAVAGIAAPHWDEKARPRFAGNGEGSGLAERMRAAVEAI
                     AQRCAEIALAAAAATPARGRRGATTRGERRTPHAERRTGPAAPVRVSGGLTRCRYLLQ
                     AQADLLGRPVAVTESRDATAVGAALLAAPIAPGQDRGGRGAETIHHPIASAARAAALR
                     RQWSRAVYRGADPSI"
     gene            complement(3473..4054)
                     /locus_tag="VFD06_00345"
     CDS             complement(3473..4054)
                     /locus_tag="VFD06_00345"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZN02012.1"
                     /translation="MRLAAAAALALVVATLAGPAALLRAAASTTPNAPASPRDRYQRR
                     FTGKPAPDFTLSDIHGNTVTLSHERGKVVLLNFWYSSCVPCRKETPDLSTLHKAYVGH
                     GFKVLGINLDDLLIPDAGHASLKVFLQEFKPPYPILLGSSDVLEAYGGVPVQPISFLI
                     GRDGKVAKVFWGAYPGAVFEKAIRAQIEGSAPR"
     gene            complement(4051..>4876)
                     /gene="lysS"
                     /locus_tag="VFD06_00350"
     CDS             complement(4051..>4876)
                     /gene="lysS"
                     /locus_tag="VFD06_00350"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZN02013.1"
                     /translation="YDTGARICREVFQSPPPFPVTYEWIAFKGMGDMSKSKGNVLSVH
                     RMLEVVPPEVLRYLIVKTRPMRSISFDPGLPLLALVDEFDDAASSARDPRALSLSGAD
                     QFKPIGVPFRHLVNVVQMADCDLDQAVVILRRNGYPIEDEAAAKERAGYAIRWIREFA
                     PEEAKFSVQRSLPEAARDLGPRERDFLRRLADKLAPGQSGEEIHALVYDVAKEAGLEK
                     TTAAFEAIYRAFLGRTRGPRAGWFLAFLDRSFVLARLREAGAAPAASAGAAPGAGA"
BASE COUNT          719 a         1810 c         1795 g          552 t
ORIGIN      
        1 acgcaagcgc gcgccgcttc agaatccgga tccccccatc gtccgccacc agaggatcgg
       61 cgccgaactg cgccgccgtc tcgtagtgcg cctccaccca gtcgaccacc cgcttgccgt
      121 acccggggac gaagccgaac cggccggtcc cgagctcctc ggtcttccgc gccacgatcg
      181 ccacgaagtc cggcgggcgg cgatcgagcg ccgcgaccag ccgatcctgg ccgaagagga
      241 ccgcctcggc ggtgacgaag ttggtgaacg ggacggtcga gaggcggcgc gtctggtaat
      301 tgagcatgat cccctcgggg agggccacga gcgtcgcgcc ctcgggcgtc gtccgctcga
      361 tccagtcgat ggcgcgggcg gcgatcggcc cccccggcgc cacctccgga gcgaaggcga
      421 cgatccgatc gccgccgcgg ccgaccggga aggtgcgcag tcccgccagc tccatcgtcc
      481 agcggacgtg gaagaggagc gcggcggcga cgatcccgag cgccgcgaac cggaagacga
      541 ctccggaacc gccgccgcgc gccgccatcc gggccggccc ctcgtgaagg agcaggacga
      601 gcgcgagcat cgtcgccggc agcgcgagcg tgaagccgta gtgatacagg tgcaggttga
      661 ggatcccctt gagggtggcg gcgagcgccc agaccgcgag cagcagcaac gcttcgcgcc
      721 gtccccgctc ggcgcgcggc acagagcctg agcgcaggag ccgccatgcc gacgccgcga
      781 tcgcgacggc ggcgacgagc ggcagggcgc gcggcagctc gacccagatc gacgctccgg
      841 ccgccgtgac gacgaagagg aaggcgagcc ccgccgcggc cccccagagg accgggcggc
      901 gcgcccgcgg cgcgaggagg cgatcggtga cgacgagcat cagcacgccg aacccgaatg
      961 tcgccgcgga gcgggtgagg ccgagaaggt tccccgggag atcgtcgatg ccgaggacgt
     1021 gccggtggaa cgcgaaccgg gtcacgcctc caccgagcgc caggaacccg gcggcggtgg
     1081 cgcgcgccgc ctcgcccggc ggcaggtagg tcaggaacca caggaagaag agcagtgacg
     1141 gaacgaccgc cgcggcggcg cacggcagca acccgcgcag tcggcggccc cggtcctctg
     1201 cccgcagcgc cgcgacgaac accgccgccg ccgcgaccgc cagggccgcc accgccgcct
     1261 cgaccttggt gaggagcgcg agacccagga ggagccccgc ggcggcggcc gaacgccggc
     1321 acccgcgccg caggaatcgt ccaatcaaaa ggaccatccc ggcgccgaac aggaggccgt
     1381 gggtcgcttc gtgcgtgtat ggggcgagga agttgtagtt gccgaccatg acatactgcg
     1441 cgaaggcgaa gaggcacagg aacgccgccg ccgcgaccca ccccgtcagc cggtcggagg
     1501 cgcggcggac gaggcgcgcc aggatccacg tgagcagcac gacgaggagc agattcgcgc
     1561 cgatgagggt cgagaacgac accccgaaga cccggaagag cagcgcgttg aggtgctgcg
     1621 acagcggccc atggaagtag agaacgtcgg ttccgagccg ctcccccgcc gccagacgcc
     1681 aggggatata gagctgctgc ccgaaatcga tgagcaggtc gggccagcgg cgccaggtga
     1741 cgccggcgag gcacgccagg agcgccagca gcggcgccgc cgcggcgatc gtccggagtc
     1801 ggcgcgccgg gaccgcgcgg cgggcggagg cccgggcctc actcgtcatc gagccacctc
     1861 ggacaggctc ccagcctccc gcaccgcagc cggcggcgcg ccggccgcgt cattctagga
     1921 gagaaacgat gcgcgtcggt ctcgacatcg gctcgtcgag cgccaaggcg ctcgtcgtcg
     1981 acgccgccgg ccgcgcgcgc cgcgccgtcc gccgcacctt tcccacccgg cgcgatcgcg
     2041 gccgcgtcga gcacgacggc gagcgcgtgg ccgccgcgac cctcggtgcg ctcgaggacc
     2101 tcgctccttc gctcccggcc cgcgccgccg tcccgctcgg gttcgcgtcg cagcgctcca
     2161 cgtttctctt ctggagccgc gacaccggcc ggcccctgac gccggcgatc tcgtggcagg
     2221 acctgcgcgc cggggggatc aaccgcggcg tccgccgccg ggcggagcgg acggggatcg
     2281 acatcggcca ggtcaccggg ctccggccga gcgcccacta cgccgccccc aagatcgcct
     2341 gggcgctgcg ccgcgtgccg ggcctcgggc ggcaggtcgc ctccgggcgg gcgttgtggg
     2401 ggacgctcac caccttcatc gtctggcgcc tgacacgtgg aggggtgtac gccgtcgatc
     2461 acgccaacgc gcagcggacg tctctcatgg acctcgcgtc gctcgcgtgg gagccgcgcc
     2521 tcttcgagat ctacggcctc gaggcgctgc tcgacgcgcc ggccctgccg gccctcaccc
     2581 cgacctgtct caccactccg atcgacgtgg cgatcggcgg gcggaggctg gcgctcgcgg
     2641 cggtcaccgg cgaccagcag gcggcgctcg tcgggctgcg ctgccggcgg cgcggcgaca
     2701 tggcgatcaa ctacggatcg ggggcgttcg tcctgcgctg cgtcggcggc acgccgctcc
     2761 gccggccggg gctcctgacg acgctcgtca cctcgcgccg caccctcccg gcctcggggc
     2821 gcggcggagg actgacggcg agctacgcgc tcgagggacc ggtgaacgcc gccgccaccg
     2881 ctctcgactg ggtcgccgcc cgcatgcacc ggcgcgtcca caccgccgat ctcgatcggc
     2941 tgctcggtcc gtgggacccg cgcgcgcgcc gcgcgatcca cttcctgccg gcggtcgcgg
     3001 ggatcgccgc cccgcactgg gacgagaagg cgcggccgcg gttcgccggg aacggggaag
     3061 ggtccggcct cgcggagcgg atgcgggcgg ccgtggaggc gatcgcgcag cgctgcgccg
     3121 agatcgccct cgccgccgcg gccgcgaccc cggcgcgcgg ccggcgcggc gccacgacgc
     3181 gcggcgagcg gcgcacgccg cacgcggaga ggcggaccgg acccgcggcg ccggtgcggg
     3241 tgtcgggcgg gctgacgcgc tgccgctacc tgcttcaggc ccaggccgat ctgctcggac
     3301 ggccggtggc ggtcaccgaa tcgcgggacg caacggccgt gggggcggcg cttctggccg
     3361 cgccgatcgc gccggggcag gatcgcggcg ggcgcggggc ggagacgatc caccatccga
     3421 tcgcgtcggc ggcgcgggcg gcggcgctgc ggcggcagtg gagccgggcg gtctaccggg
     3481 gcgcggaccc ttcgatctga gcccggatcg ccttctcgaa caccgccccc gggtaggccc
     3541 cccagaacac cttcgccacc ttcccgtccc ggccgatgag aaaactgatc ggctgcaccg
     3601 ggacgccgcc gtaggcctcc aggacgtcgc tgctgccgag gaggatcggg tacggcggct
     3661 tgaactcctg caggaacacc ttgagggagg catgaccggc gtccgggatc agcagatcgt
     3721 cgaggttgat gccgagcacc ttgaacccgt gcccgacgta ggccttgtgc agcgtcgaga
     3781 gatcgggcgt ctccttccgg cacggaacgc aggaggagta ccagaagttg agcaggacca
     3841 ctttcccccg ctcgtgcgac agcgtgacgg tgttgccgtg gatgtcggag agggtgaagt
     3901 cgggggccgg cttgccggtg aagcggcgct ggtagcgatc gcgcggcgag gccggggcgt
     3961 tcggagtcgt cgacgccgcg gcgcgcagga gcgccgccgg cccggccagg gtcgcgacca
     4021 ccagcgccag cgccgcggcg gccgcgagcc tcacgccccc gctcccggcg cggcgcccgc
     4081 cgacgcggcg ggagcggccc ccgcctcccg caggcgcgcc agcacgaacg aacggtccag
     4141 gaacgccagg aaccagccgg cgcgcgggcc gcgcgtgcgc ccgagaaacg cccggtagat
     4201 cgcctcgaac gcggcggtcg tcttctccaa ccccgcctcc ttggcgacat catagacgag
     4261 cgcgtggatc tcctcgccgc tctgtcccgg cgcgagcttg tcggccagcc ggcgcaggaa
     4321 gtcccgctct cgaggtccga gatcgcgggc cgcctcgggc agggagcgct gcaccgagaa
     4381 cttcgcctcc tcgggagcga actcgcggat ccagcggatg gcgtagccgg cccgctcctt
     4441 cgccgccgcc tcgtcctcga tcgggtagcc gttccgcctc agaatgacga ccgcctggtc
     4501 gaggtcgcag tccgccatct gcacgacgtt caccaggtgc cggaacggca cgccaatcgg
     4561 cttgaactgg tcggcccccg acagcgacag cgcccgcgga tcgcgggcgc tcgacgccgc
     4621 gtcgtcgaac tcgtccacga gcgccaggag cggcagcccc gggtcgaagc tgatcgagcg
     4681 catcggccgc gtcttgacga tgaggtagcg caggacctcc ggcggcacca cctcgagcat
     4741 ccggtgcacc gacaggacgt tccccttgct cttcgacatg tcccccatcc ccttgaaggc
     4801 gatccactcg taggtgacgg ggaacggcgg aggggactgg aacacctcgc ggcagatgcg
     4861 ggcgccggtg tcgtac
//
LOCUS       DASRIQ010000016         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3655881, whole genome shotgun sequence.
ACCESSION   DASRIQ010000016 DASRIQ010000000
VERSION     DASRIQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3655881"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /gene="eno"
                     /locus_tag="VFD06_00355"
     CDS             complement(<1..1004)
                     /gene="eno"
                     /locus_tag="VFD06_00355"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZN02014.1"
                     /translation="MVKISDVAAREILDSRANPTVEVEVILDDGSVGRAAVPSGASTG
                     SHEALELRDGDPGRFRGKGVQKAVHNVISVLRPAVFGLDPGNQEVLDARLREVDGTLD
                     CSHLGANALLGVSLAAARAAAASRRIPLYLHLGGEGARDLPVPLFNILNGGAHADNNV
                     DIQEFMVLPVGASTFREALRMGAEVFHALRDILRKAGLRSGQGDEGGWAPDLKSNTEA
                     VEVVLQAIEQAGYRPGEQMAIGLDVAASELARDGAYLLEAEQPPRRTSDEMVAFYEGL
                     VSRYPIVSIEDGLAESDWSGWERLTAALGGRVQLVGDDLFVTNVSLLEEGIRRGVANS
                     "
     gene            complement(1012..2409)
                     /locus_tag="VFD06_00360"
     CDS             complement(1012..2409)
                     /locus_tag="VFD06_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02015.1"
                     /translation="MAPPPPCRGTIADEVTFASVRHRAAAFGAALAGLPGRGRAGVVV
                     GHDARFLADRFAAEAAAVIAARGLPVLLADAPLPAPALAHAIASGRRAGGLYVGGGDL
                     PAEETGVALYDAAGAPADDDLLEQVNRLLLDPETAARTARRRTPPVRRVRPQGAYLRS
                     LLRAVPAARIWRARLRLACDARHGAAAGWITAALGRVSAALEALHDDPRPDFGGLPPT
                     CSEPDLKALGRSVRRARRHLGLAIDADGTHCGAVDERGAPIPPGALAALLADRLLGEG
                     RVRGGLARGVAATHLLDDVAAAHGATVVEVPFGAGPLAAALGPARAGLAVDEAGGIAL
                     PGHARERDGILVALLAAEAAAVEKRPLRDQISDLHRRLGPRLGRRIDYHVDPSARERA
                     LLRLQDVPARFAGRPVRAVAASESRKWILADGSWVLFRAAADDGPLRCHLEARSARDL
                     ETLTAAAREWVAGGR"
BASE COUNT          335 a          965 c          918 g          289 t
ORIGIN      
        1 atcgagttgg ccacgccgcg acggatcccc tcctcgagga gcgaaacgtt ggtgacgaag
       61 aggtcgtcgc cgaccagctg cacccgcccg cccagcgcgg cggtcagccg ctcccagccg
      121 gaccagtccg actccgccag cccgtcctcg atcgacacga tcggatagcg tgagacgagc
      181 ccctcgtaga acgccaccat ttcgtcgctc gtccggcggg gcggctgctc cgcctccagg
      241 aggtaggcgc cgtcgcgcgc gagttcgctc gccgccacgt cgagcccgat cgccatctgc
      301 tcgccggggc ggtagccggc ctgttcgatc gcctgcagca ccacctccac cgcctcggtg
      361 ttggacttga gatcgggggc ccagccccct tcgtccccct ggcccgaccg cagccccgcc
      421 ttccggagga tgtcgcgcag cgcgtggaac acctccgccc ccatgcgcag cgcctcccgg
      481 aaggtggacg ctccgaccgg caggaccatg aactcctgga tgtcgacgtt gttgtcggcg
      541 tgcgctccgc cattgaggat gttgaagaga gggaccggga gatcgcgcgc cccctcgccg
      601 ccgaggtgca gatagagagg aatccggcgg ctggcggccg cggcgcgggc ggcggccagc
      661 gacacgccga ggagggcatt ggcgccgagg tgcgagcagt cgagcgtgcc gtccacctcg
      721 cggagccgcg cgtcgagcac ctcctggttg ccgggatcga ggccaaagac ggcggggcgc
      781 aggaccgaga tcacgttgtg gacggccttc tgcaccccct tgccgcggaa acggccggga
      841 tcgccgtcgc gcagctcgag cgcctcgtgc gatccggtcg aagctccgga ggggaccgcc
      901 gcccggccga cgctgccgtc gtcgaggatc acctccacct cgacggtggg gttggcgcgc
      961 gagtcgagaa tctcccgcgc cgcgacgtcg gagatcttca ccatgcccgc ttcatctccc
     1021 ccccgcgacc cactcgcgcg ccgcggcggt cagcgtctcg agatcccgcg cgctacgcgc
     1081 ttcgaggtgg cagcgcagcg gcccgtcgtc cgccgccgcg cggaacagca cccacgagcc
     1141 gtccgcgagg atccacttgc gcgattcgga ggccgcgacc gcgcgcaccg gacgaccggc
     1201 gaagcgcgcc ggcacgtcct gcagccgcag cagcgcgcgt tcgcgcgccg atgggtcgac
     1261 atgatagtcg attctccggc cgagccgcgg ccccaatctg cggtggagat cggaaatctg
     1321 atcgcgcagc ggccgtttct cgaccgccgc cgcctccgcc gcgaggagcg cgacgaggat
     1381 tccgtcgcgc tcccgggcgt gccccggaag cgcaatcccc cccgcctcgt cgaccgccag
     1441 cccggcgcgc gccggcccga gggccgcggc cagcggaccg gcgccgaacg gcacctcgac
     1501 gaccgtcgcg ccgtgcgccg cggcgacgtc gtcgagcagg tgcgtcgccg cgacgccgcg
     1561 cgcgagaccg ccgcgcaccc gcccttcgcc gagcaggcgg tcggcgagga gcgcggcgag
     1621 cgcgccaggc gggatcgggg cgccgcgctc gtcgacggcg ccgcaatggg ttccgtcggc
     1681 gtcgatcgcc aggcccaggt gccggcgggc ccggcggacg ctccggccga gcgctttcag
     1741 atccggctcc gagcaggtgg gcgggagccc gccgaagtcc gggcgcggat cgtcgtggag
     1801 tgcctcgagc gccgcggaca cgcggccgag ggccgccgtg atccatcctg ccgcggcgcc
     1861 gtggcgcgcg tcgcacgcca gccgcagccg ggcgcgccag atccgggccg ccggcaccgc
     1921 gcgcaagagg cttctgagat acgcgccctg cgggcggacg cgccgcacgg gcggagtacg
     1981 gcgccgggcc gtcctggccg ccgtctccgg atcgaggagg agccggttca cctgctccag
     2041 gagatcgtcg tcggccgggg cgccggcggc gtcgtagagc gccacgccgg tctcctccgc
     2101 cggaaggtcg cccccgccga cgtacagccc gccggcgcgc cgcccactgg cgatcgcatg
     2161 cgccagggcg ggcgccggga gcggtgcgtc ggcgagcagg accgggagac cgcgcgcggc
     2221 gatgacggcg gccgcctccg ccgcgaagcg atcggcgagg aagcgggcgt cgtggccgac
     2281 gacgactccg gcccgccccc ggccgggcag gccggcgagc gccgcgccga aggcggcggc
     2341 ccggtgccgc accgacgcga aggtgacctc gtcggcgatg gtgccgcggc agggcggagg
     2401 cggcgccatc atgcgcgccg ctcacgcgcc agcgcggtat aatccccacc atgcgcgatg
     2461 cccccgcgat cgtcgtcctc gtcaccgccg ccaccgagaa ggaagcg
//
LOCUS       DASRIQ010000017        10098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3130618, whole genome shotgun sequence.
ACCESSION   DASRIQ010000017 DASRIQ010000000
VERSION     DASRIQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10098
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3130618"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFD06_00365"
     CDS             complement(<1..601)
                     /locus_tag="VFD06_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN02016.1"
                     /translation="MIYLDHAATTFPKPEEVYRDMDAFVRRAGANPGRGGHRLAQEAE
                     RMIDETRLLVARLAGATRPERVVFGLNASDGLNMAIKGTLRTGDHVVTSVLEHNSINR
                     PLERLERDGTIAVTRLGAGPDHRLDPDDVARAFRPTTRLVALTHASNVTGTIQPVAEI
                     GRRVREHGALFLVDAAQSAGVVPLDMERDAIDLLAFTGHK"
     gene            695..6367
                     /locus_tag="VFD06_00370"
     CDS             695..6367
                     /locus_tag="VFD06_00370"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZN02017.1"
                     /translation="MTRHAAVPIATLLASILTSGALPARGARPAIARAPAAAAVVCDP
                     GEILGYLYVNEETTDRTAESADNVVSGFAAHADGCVEVLTGSPWPTGGLSASGLVLIA
                     APRLDIAARGDRLYALNRGSDDVAAFRVGAEGDLVPLDGSPFDTGGRGPQGMAVSPDG
                     RWLFVAHRGDRRLTTLRLPPDGRPSLAASFDLDAEANGLVVSRDGRFLVAALSHLARV
                     AVLEIAPDGSLRHAPGSPARADLNGADGVVLAPGGADVYVSAADLDRLAVSRYRLGED
                     GAIDRVAGSPYFGPGGAGNVLAMLPDGAHLLASQTVRNALTAFRVDASGGLTPTPVSP
                     FPTGFGQQLPTGMAFDPLGQFLWVVHADGSGIASLRHLGNGSFAPAAAPTPSRVDGVP
                     LNGLAFVPRQGSDADADGAPFPADNCGAAANPEQSDADGDGVGDACDDCPLVSDPGQR
                     DADRDGAGDACDPDRDGDGVTDAADRCPDRPDPGQTDDDHDGVGDACDNCPAVPNEGQ
                     EDADRDREGDACARPFVLIGRLYVQAESPENAIAGYDVDTLGRLRRIAGSPFATGGNG
                     PVVTTLFAPKRLEWSPLMPTLLFASNEGSDNLSVMRIDADGGLALATGRLRASGGSRP
                     AGLAIRPHTLQLVVGHLGSANLTLYQIQPSSGAIFGIAGGTIPTQGRPSGMAFTPDGR
                     FLEVALTDLGPARTLKIDPPYQFLPETGIGDRNGRAATPLFNRAGDRLYLASSTAGES
                     IVSGWAYDDEGMPERLLRSPQTAGGLNSNILFMHPDRRHLYVSQQRSNTIGVLRIEPS
                     GELAPLGGPFPPARFAAEPVGLAADASGRFLFASYAQSNTVAAYRVTPEFGVEALGEA
                     EKTGAPAGRPLAGVVFVPAGDEDDDGRSALEDNCPGVANPGQADADGDGTGDVCDTCP
                     LDAGDNGDSDGDGIGDACDEDQDGDGIGDAGDLCPLDADADQTDTDGDARGDRCDRCR
                     LDPFDDADADGSCADFDNCPVVYNPFQENSDVDRHGNDCDNCPFLWNDDQADIDAAEG
                     GDACQRGFQQTGFLFVDTDAIDNSLATWETKSTGRLMPTPGSPYFTAGNGHPNNPSRT
                     SAPAIGFVERGRFLFVLNPQSRDISVFHLASDGLPRSIPGSPFPTGFEQPIGLVADPG
                     ARFVWVAAREPDGGRLRVFDVARSGRLAPGAGEVVALEALPDGLALSPDGRWLAVSIP
                     EAGRVLLFGVPEGELAEAVSVPGGPPRWDGAIPGLPRPGPLSFALTPSGRLLLDVGSA
                     SPDRAVVAALDLASGMPAGPAFDLGVTGGVSALAGSSSPEPGSEGAMRLFATLPGANA
                     VAVVEGLHGGGAAVQPSGSPFPLPAGAQDPVGLAVDPPWLHITARQSGNLATFRVEAG
                     GALAVTPVPPTSVGASGGHPAAGVVRFVLDDRDDDGVGPLADNCPSAANPAQEDRDGD
                     GAGDACQPEISLGPILAGPYLPPAGQAIPEAQTALAAALHLEEPQEEPLEGRVVIAAR
                     GGVSATLLDAAAGGALTPIDCRRALRPAAGIGGGIAYVYGSVGDAFLFDQDALLVCDD
                     GRPDYELGEGRCGTPGQPFTTSLILSLRTPPFDVCARPIADRSVSFDLRLEAIEPDHL
                     DLSAVIEQAIVDAPWDGRPDPIPLSSLGSAAGPFTLAITASDGDTPPVRARASFQRAG
                     AEALIFGAAPLLPDLGDRRVECAASGGTPVDLDSVGARDPDGEALSFFWLEEDGAGEV
                     RLLATGPAPRVSLPYGEHRLVLDIVDPTGLLARGSFVVTIGDTRPPEILLLAADPEIL
                     WPPDHRLVPVRLRLEARDACFEAAEARLLDVTSSEPDDAPGGGDGRTVGDIVPGSSGE
                     GEWALVLRAERLRPGPGRTYRARFVVADAAGNERQATIEIAVPRSAPD"
     gene            6390..6755
                     /locus_tag="VFD06_00375"
     CDS             6390..6755
                     /locus_tag="VFD06_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN02018.1"
                     /translation="MAKIPGWYQKQRKRYPEVFGAYERLGAACRGAGPLDEKTAALAK
                     LAIAVGARLEGAVHSHVRRSMEAGATADECRHVVLLATTTIGFPAMMAALSWVEDVAG
                     GKPPAPNASRRASRQGPKS"
     gene            complement(6752..6955)
                     /locus_tag="VFD06_00380"
     CDS             complement(6752..6955)
                     /locus_tag="VFD06_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02019.1"
                     /translation="MPVLSRLDAQRVERPCSPPRLETLHPIAHARVEDVYEHPTVAGL
                     AAALRISPAGRRAAATSRVAVGG"
     gene            complement(7041..9194)
                     /locus_tag="VFD06_00385"
     CDS             complement(7041..9194)
                     /locus_tag="VFD06_00385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN02020.1"
                     /translation="MPSAYRRPFRGSPAPSFVLALAVLIPAIGSPAVAAPTSGTEPHP
                     FSVHDMVAMERLADPRLSPDGTWIVYSQRSYDWDANATSINLWIVPAAGGAPRRLTAG
                     ASRDTSPRWSPDGKVIAFVSDRGESSQIWTIDPTGGEAAALTRFPLDVDNPQWAPSGR
                     RLAFTAEVYPDCPDFQCTADRDKKATADPVKARLFDRLPIRHWDTWEDGKRSHIFVWT
                     PGGIGGEPGGAKPDFTPVDIMKGIDADAPPKPFGGAEEFAWSPDGRSIAFTAKVRPDD
                     AAWSTDLDIWLAPADGRGAPRCLSESNEAQDSNPAFSPDGKTLAWLAMTRPKFEADRQ
                     RVVLYDLASGTIRSVTDKWDRSASSLSWAPDGRRLFVTADETGRLKIFSLALPSGTGD
                     ARVAVVAGDGHNVGVDAGRDRIVWLHDSMVSPAEVWSARHDGAEARALTRVNAARMAS
                     ARVSPLEEFWFTGAGGDRVHGWLLKPVGWKEGTKYPVAFLIHGGPQGAWTDQFHYRWN
                     FEAFAGAGYLTVAINPRGSTGYGQSFTDQISNDWAGKPYEDLMKGLDHLLATRRDLDA
                     DRMCALGASYGGFMINWMQGHTDRFKCFVSHDGELDVKSSYYSTEELWFPEWDLGGTP
                     WENPEGYARVSPEAHVARWKTPMLVVHGAKDFRLPETEGFSVFTVLQRRGIPSQFLYF
                     PDENHWVLKPRNSVRWYDTVLAWLDRWTGGTHRSG"
     gene            9440..9601
                     /locus_tag="VFD06_00390"
     CDS             9440..9601
                     /locus_tag="VFD06_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02021.1"
                     /translation="MIKFDSIEPPQPRIAPMIAPGLNAYDPVNNGTRKPTNDRWREVP
                     EIKRPQTRL"
     gene            complement(9704..10057)
                     /locus_tag="VFD06_00395"
     CDS             complement(9704..10057)
                     /locus_tag="VFD06_00395"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZN02022.1"
                     /translation="MPEKLPVLDAAAIESRLAALPGWKVENGWLRRKYNTDGWPTTLM
                     LINAVGFLCEAAYHHADLEVTWGKLWVKLMTHSSGGITERDFALAKEIDRLALWRPAE
                     GSAMEGTPNKWVHAK"
BASE COUNT         1341 a         3618 c         3697 g         1442 t
ORIGIN      
        1 ccttgtgtcc ggtgaacgcg agcaggtcga tggcgtcacg ctccatgtcg agcggcacca
       61 ctccggcgct ctgcgccgcg tccacgagga agagcgcccc gtgctcccgg acgcgccggc
      121 cgatctccgc caccggctgg atcgtgccgg tgacgttcga cgcgtgcgtc agcgccacga
      181 gccgcgtcgt cggccggaac gcccgggcca cgtcgtccgg atcgaggcgg tgatccggcc
      241 cggcgccgag gcgggtgacg gcgatcgtcc cgtcccgctc caggcgctcg agcggccgat
      301 tgatggagtt gtgctccagg accgaggtga cgacgtggtc gcccgtccgg agcgtcccct
      361 tgatcgccat gttcaggccg tcggaggcgt tcaggccgaa gacgacccgc tcgggccgcg
      421 tggcgccggc gagccgggcg acgaggaggc gtgtctcgtc gatcatccgc tccgcctcct
      481 gcgccaggcg gtgccctccg cgtcccgggt tcgcgccggc gcggcggacg aaggcgtcca
      541 tgtcgcggta cacctcctcc ggcttcggaa aggtggtggc ggcatggtcg aggtagatca
      601 tcgcggcccg gatcgtatca gagggcaccg aagcttccgc atcgcccgca ttgcgggcgt
      661 tgacgccgga ggggaggcgg cgtaagaggg ggacgtgacc cgccatgcgg ccgtcccgat
      721 cgcgacactg ctcgcctcga tcctcacgag cggcgcgctg ccggcgcgcg gggcgcggcc
      781 ggcgatcgcg cgcgctccgg cggcggccgc cgtggtgtgc gatcccgggg agatcctcgg
      841 atacctctac gtcaacgagg agacgaccga ccgcaccgcc gagagcgccg acaacgtcgt
      901 ctccggattc gcggcgcacg ccgacgggtg cgtcgaggtc ctgacggggt cgccgtggcc
      961 cacgggagga ctgtcggcct ccggcctcgt cctcatcgcc gcgccgcggc tcgacatcgc
     1021 cgcgcggggg gatcgtctct acgcgctgaa ccgcgggtcg gacgacgtgg ccgccttccg
     1081 cgtcggagcg gagggggatc tggtgccgct ggacggttcg ccgttcgaca ccggcggccg
     1141 ggggccgcaa gggatggccg tcagccccga cgggcgctgg ctcttcgtgg cgcatcgcgg
     1201 cgaccggcgc ctgacgacgc tgcggttgcc gccggacggg agaccgtccc tcgccgcgtc
     1261 gttcgacctc gacgccgaag ccaacggcct cgtggtgagt cgcgacgggc gcttcctggt
     1321 cgcggcgctg tcgcacctgg cgcgggtcgc ggtcctcgag atcgccccgg acggaagcct
     1381 gcgtcacgcc cccggctccc cggcgcgcgc cgatctcaac ggcgccgacg gcgtggtcct
     1441 cgcgccgggc ggggccgatg tgtacgtctc cgcggcggac ctcgaccgcc tcgccgtcag
     1501 ccgctatcgc ctgggggagg acggggcgat cgatcgcgtc gccggatcgc cgtacttcgg
     1561 tccgggcggg gcggggaacg tcctggcgat gctcccggac ggcgcgcacc tcctggcgag
     1621 ccagaccgtc cggaacgccc tgaccgcctt ccgcgtggac gcttcgggcg ggctcacccc
     1681 gacgccggtc tccccgttcc cgaccggctt cggccagcag ctcccgaccg gcatggcctt
     1741 cgatccgctc gggcagttcc tctgggtcgt gcacgcggac ggcagcggga tcgcctcgct
     1801 gcggcacctc gggaacgggt ccttcgcccc ggccgcggcg cccacgccga gtcgcgtgga
     1861 cggcgtgccg ctcaacgggc tggcgttcgt gccacgccag ggctccgatg ccgacgcgga
     1921 cggcgcgccg ttcccggccg acaactgcgg cgccgcggcg aacccggagc agtccgacgc
     1981 cgacggagac ggggtcggcg acgcctgcga cgactgcccg ctcgtctccg acccggggca
     2041 gcgggacgcc gatcgcgacg gcgcggggga cgcctgcgat ccggatcggg acggcgacgg
     2101 cgtcacggat gccgccgacc ggtgccccga tcgaccggac cccgggcaga ccgacgacga
     2161 ccacgacggc gtcggcgacg cctgcgacaa ctgccccgcc gtccccaacg agggacagga
     2221 ggacgccgat cgcgatcgtg agggggacgc gtgcgcgcgt cccttcgtgc tcatcggacg
     2281 gctgtacgtt caggcggagt cgccggagaa cgccatcgcc ggctacgacg tggacacgct
     2341 cggccggctg cggcggatcg ccggatcgcc gttcgccacc ggcgggaacg gtcccgtggt
     2401 gacgacgctg ttcgcgccga agcggctcga atggagcccc ctgatgccga cgctcctgtt
     2461 cgcgagcaac gaggggagcg acaatctgag cgtcatgcgg atcgacgcgg acgggggatt
     2521 ggcgctcgcg accggccgcc tgcgcgcctc gggggggtcg cgtcccgccg gcctcgcgat
     2581 ccgaccgcac acgctccagc tcgtcgtcgg tcacctcgga tccgcgaatc tcaccctgta
     2641 ccagatccag ccgagcagcg gcgcgatctt cggcatcgcc ggcgggacga tcccgaccca
     2701 gggccggccg agcggcatgg cgttcacgcc ggacggccgt ttcctcgagg tggcgctgac
     2761 ggatctcggg ccggcgcgga cgctgaagat cgatccgccg taccagttcc tgccggagac
     2821 cggcatcggc gaccgcaacg ggcgcgcggc gacgccgctg ttcaaccgcg ccggcgaccg
     2881 cctgtacctc gcctcctcca cggcggggga gtcgatcgtc tccggatggg cgtacgacga
     2941 cgaggggatg ccggagcggc tgctgcgctc gccgcagacg gcgggagggc tgaactcgaa
     3001 catcctgttc atgcacccgg accggcggca tctctacgtg tcgcagcaga ggagcaacac
     3061 catcggcgtc ctgaggatcg agccgtcggg ggagctcgcg cctctgggcg ggccgttccc
     3121 accggcccgc ttcgccgccg agccggtcgg actggccgcc gacgcttcgg gccggttcct
     3181 gttcgcgtcg tacgcgcagt cgaacaccgt cgccgcgtac cgtgtcacgc cggagttcgg
     3241 ggtcgaagcg ctcggcgagg cggagaagac cggggcgccc gccggacgtc cgctcgccgg
     3301 cgtcgtcttc gtgccggccg gcgacgagga cgacgatggg cgctcggccc tggaggacaa
     3361 ctgtcccggc gtggcgaacc cggggcaggc ggacgccgac ggcgacggga ccggggacgt
     3421 gtgtgacacg tgcccgctgg acgcgggcga caacggcgac tcggacggcg acggcatcgg
     3481 cgacgcctgc gacgaagacc aggacggcga cggcatcggc gacgccggcg atctctgtcc
     3541 tttggacgcg gacgccgatc agaccgacac cgacggcgac gcgcgggggg atcgctgcga
     3601 ccgctgccgc ctcgacccgt tcgacgacgc ggacgccgac ggctcgtgcg ccgacttcga
     3661 caactgcccg gtcgtgtaca acccgttcca ggagaacagc gacgtcgatc gccacggcaa
     3721 cgactgcgac aactgcccgt tcctgtggaa cgacgatcag gcggacatcg acgcggcaga
     3781 gggcggggac gcctgccagc gcggcttcca gcagaccggc ttcctgttcg tcgacaccga
     3841 cgccatcgac aactcgctcg ccacctggga aaccaagagc accggtcggc tgatgcccac
     3901 gcccggcagc ccctatttca ccgccgggaa cggacacccg aacaatccgt cgcgcacgag
     3961 cgccccggcg atcggattcg tcgagcgcgg ccggttcctg ttcgtgctga acccgcagag
     4021 ccgcgacatc tccgtcttcc atctcgcgtc ggacgggctg ccgcggtcga ttccggggtc
     4081 ccccttcccg acggggttcg agcagccgat cggcctcgtg gccgatccgg gcgcccggtt
     4141 cgtctgggtg gcggcccgcg agccggacgg cggccgcctg cgcgtcttcg acgtcgcccg
     4201 gtcgggacgc ctggcgccgg gggcagggga ggtcgtggcg ctcgaggcgc tccccgacgg
     4261 tctggcgctg agccccgacg ggcggtggct cgccgtgtcg attccggaag ccggccgcgt
     4321 cctgctcttc ggcgtgcccg aaggagagct tgcggaggcg gtgtcggtgc cgggcgggcc
     4381 gccgcgctgg gacggcgcca tccccggtct gccccgcccc ggaccgttgt cattcgcgct
     4441 gacgccttcc ggtcgtctgc tgctcgacgt cggatccgcc tcgcccgatc gggcggtcgt
     4501 cgccgcgctc gacctcgcca gcggaatgcc ggccgggccg gcgttcgacc tcggcgtcac
     4561 cggcggagtc tctgccctgg ctggatcgtc gtcgccggag cctggaagcg aaggcgcgat
     4621 gcgtctgttc gcgaccctgc cgggcgccaa cgccgtcgcg gtcgtcgagg ggctccacgg
     4681 cggtggtgcc gcggtccagc cctccggctc gccgttcccg ctgccggccg gggctcagga
     4741 cccggtcggg ctggcggtcg atccgccgtg gctgcacata acggcgcggc agtcgggcaa
     4801 cctggccacc ttccgggtcg aggccggcgg cgcattggcg gtgactccgg tgccgccgac
     4861 ttcggtcggg gcctccggag ggcacccggc ggccggggtg gtgcgattcg tcctggacga
     4921 tcgcgacgac gacggggtcg gcccgctcgc cgacaactgt ccgtcggccg cgaatccggc
     4981 gcaggaggac cgggacggag acggcgcggg ggatgcctgc cagccggaga tctctctcgg
     5041 gccgatcctc gccgggccgt acctgccgcc ggcgggacaa gcgattccgg aggcgcagac
     5101 cgcgctcgcc gccgcgctcc atctcgagga gccgcaggag gagccgctcg agggacgggt
     5161 ggtcatcgcg gcgcgcggcg gcgtgtcggc gacgcttctg gacgccgccg cgggcggggc
     5221 gctgaccccg atcgactgcc ggcgggccct gcgtcccgcg gcggggatcg gagggggcat
     5281 cgcttacgtc tacggctccg tcggcgacgc cttcctgttc gatcaggatg cgctgctcgt
     5341 ctgcgacgac gggcggccgg actacgagct gggggagggg cggtgcggaa cgccgggcca
     5401 gccgttcacc acctccctga tcctgagtct ccgcaccccg ccgttcgacg tgtgcgcgcg
     5461 gccgatcgcc gatcggtccg tctcgttcga tctccggctc gaggcgatcg agccggatca
     5521 tctcgacctt tccgccgtga tcgagcaggc gatcgtcgac gccccgtggg acgggcgccc
     5581 cgatcccatc ccgctctcct ccctcggctc cgcagccggc cccttcaccc tggcgatcac
     5641 ggcgtcggac ggcgacacgc cgccggtccg ggcgcgggcc tcgttccagc gcgccggcgc
     5701 cgaagcgctc atcttcggcg cggcgccgct cctgcccgac ctcggagatc ggcgggtcga
     5761 gtgcgccgcg tccggcggca cgccggtcga tctggacagc gtcggggcgc gcgatcccga
     5821 cggggaggcg ctctcctttt tctggctcga agaggacggg gcgggggaag tccggctgct
     5881 cgccaccggg cccgcgccgc gggtcagcct tccctacggc gagcatcgtc tcgtcctcga
     5941 catcgtcgat ccgaccggcc tcctggcgcg cggcagcttc gtcgtgacga tcggggacac
     6001 gcgcccgccg gagatcctcc tcctcgcggc cgatcccgaa attctgtggc cgccggatca
     6061 ccggctcgtc ccggtgcgcc tgcggctcga ggcgcgcgac gcgtgcttcg aggcggccga
     6121 ggcgcgcctc ctggacgtga cgagcagcga gccggacgac gccccgggcg gcggcgacgg
     6181 ccgcacggtc ggagacatcg tcccgggatc gtcgggggaa ggggagtggg cgctggtgct
     6241 tcgggccgag cggctcaggc cggggcccgg tcggacctac cgcgcccgct tcgtcgtcgc
     6301 cgacgccgcc ggcaacgagc ggcaggcgac gatcgagatc gcggtgccgc gctccgctcc
     6361 cgactagaat ccggcacgag gaggtcggcg tggccaagat ccccggctgg taccagaagc
     6421 agcgcaagcg ttacccggag gtgttcggcg cctacgagcg cctcggcgcc gcctgccgcg
     6481 gcgcggggcc gctcgacgag aagacggcgg cgctcgccaa gctcgccatc gccgtcggcg
     6541 cgcgcctcga aggggcggtc cactcgcacg tgcggcgctc catggaggcg ggggcgaccg
     6601 ccgacgagtg ccgccacgtc gtgctcctgg cgacgacgac gatcggcttc cccgcgatga
     6661 tggcggcgct gtcgtgggtg gaggacgtgg cgggcggcaa accgccggcg ccgaacgcgt
     6721 cgcgacgggc ctcccgccag ggtcctaagt cttagcctcc cacggcgacg cgggaggtgg
     6781 cggcggcccg gcgtcccgcg ggggaaatgc gcagcgcggc ggcgaggcct gcgaccgtcg
     6841 ggtgctcgta gacgtcctcg acacgggcgt gggcgatcgg atgcagggtc tcgaggcgcg
     6901 gcggggagca aggacgctcg actcgctgtg catcgagccg agacagtacc ggcatcgtcc
     6961 ggccggcgcc atctgtggaa atcccgatcc gagtatccgc ttttccccga ggattcaggc
     7021 ggcgcgaatc ccgccggggg tcagccgctg cggtgcgtcc cgccggtcca ccggtcgagc
     7081 cacgccagga ccgtgtcgta ccagcggacg ctgttgcgcg gcttcaggac ccagtggttt
     7141 tcgtcgggga agtacaggaa ctgcgagggg atgccgcgac gctgcaggac ggtgaagacg
     7201 ctgaaccctt ccgtctccgg cagtcggaag tccttcgcgc cgtgcacgac gagcatcggg
     7261 gtcttccacc tggcgacgtg tgcctcgggt gaaaccctcg cgtaaccttc cggattttcc
     7321 cacggcgtgc cgccgagatc ccattccggg aaccacagtt cctcggtcga gtagtagctg
     7381 gacttcacgt ccagctcgcc gtcgtggctg acgaagcact tgaagcggtc ggtgtgcccc
     7441 tgcatccagt tgatcatgaa gccgccgtag gaggccccga gtgcgcacat gcggtcggcg
     7501 tccagatccc tgcgcgtcgc cagcaggtgg tcgagcccct tcatcaggtc ctcgtacggc
     7561 ttgccggccc agtcgttgct gatctgatcg gtgaatgact gcccatagcc ggtgctgccg
     7621 cgcgggttga tcgccaccgt aaggtagccg gcgccggcga aggcctcgaa gttccagcga
     7681 tagtggaact ggtcggtcca ggccccctgc ggcccgccat gaatcaggaa ggcgaccggg
     7741 tatttcgtcc cctccttcca accgaccggc ttgagaagcc agccatggac gcgatcccct
     7801 ccggcgcccg tgaaccagaa ctcctcgagg gggctgacgc gggccgaagc catgcgggcg
     7861 gcgttgacgc gcgtcagggc gcgcgcctcg gcgccgtcgt ggcgcgcgct ccacacctcg
     7921 gcgggggaga ccatcgagtc gtgcagccag acgatgcgat cgcgtccggc gtccacgccg
     7981 acgttgtgcc cgtcccccgc gaccaccgcg acgcgcgcgt cgccggtgcc ggagggcagc
     8041 gcgagggaga agatcttcag gcgcccggtc tcgtccgccg tgacgaagag ccgccggccg
     8101 tccggggccc aggagaggct cgacgccgat cgatcccatt tgtccgtcac gctccggatc
     8161 gtcccggagg ccaggtcgta gaggacgacc cgctggcggt ccgcctcgaa cttcgggcgt
     8221 gtcatcgcca gccacgcgag ggtcttcccg tccggggaga aggcggggtt gctgtcctgc
     8281 gcctcgttcg attcgctgag gcaacgcggc gcgccgcggc cgtcggccgg ggcgagccag
     8341 atgtcgagat cggtggacca ggcggcgtcg tccggccgca ccttcgcggt gaaggcgatc
     8401 gagcggccgt cgggagacca ggcgaactcc tcggcccccc cgaacggctt ggggggcgcg
     8461 tcggcgtcga tccccttcat gatgtccacg ggcgtaaagt ccggtttcgc gccgcccggc
     8521 tcgccgccga tcccccccgg ggtccagacg aagatgtggc tgcgtttgcc gtcctcccat
     8581 gtgtcccagt gacggatcgg cagccgatcg aagaggcgcg ccttgaccgg atccgcggtc
     8641 gctttcttgt cgcggtcggc ggtgcactgg aagtcggggc agtccgggta cacctcggcg
     8701 gtgaacgcca gacgccggcc ggagggggcc cactgcgggt tgtccacgtc caacgggaag
     8761 cgggtcagcg cggccgcctc gccgccggtg gggtcaatcg tccagatctg cgacgactcg
     8821 ccgcgatcgg agacgaaggc gatcaccttg ccgtccggcg accagcgggg ggaggtgtcg
     8881 cgcgacgcgc cggcggtcag gcgccgcggc gcaccgccgg cggcggggac gatccagagg
     8941 ttgatggagg tggcgttggc gtcccagtcg tagctccgct gggaatagac gatccacgtc
     9001 ccgtcgggag agaggcgcgg atcggccagg cgctccatcg cgaccatgtc gtgcaccgag
     9061 aacgggtgcg gctccgtccc cgaggtcgga gcggcgacgg ccggggaccc gatcgcgggg
     9121 atcaggacgg cgagggcgag gacgaaggac ggcgccgggc ttccccggaa cggacggcga
     9181 tacgcggacg gcatcgggcc tccttgctgg tcagggtgct gctcggggcg ggattctgcc
     9241 gcagatcggc ccgatccgga tcgaccgctg acagcctcgc gggcgtgacc cttgcccatc
     9301 ccggccggtt cgcgcttcct gagccccgct tccgaacttc ctgacctgcg atggagcgag
     9361 cgcctcattg tccggtcaca gccttgggag cagagtcgtc tcgcgccgga agagtggcgc
     9421 atggaggaag gagggcacca tgatcaagtt cgactccatc gagcccccgc agccgaggat
     9481 tgcgcccatg atcgcccccg gtctgaacgc ttacgacccg gtgaacaacg ggacccggaa
     9541 gccgacgaac gaccgctggc gcgaagtgcc tgagatcaag cggccgcaga cgagactctg
     9601 atcccgtcgc gccgtcagga gcccgtgggg gggtccggag ccgggacgcg ccgacccctc
     9661 ccgcgggctt tcgcgcgcta cgacgaaggc cttcgcgcgc gggctacttc gcgtgcaccc
     9721 acttgttcgg cgtcccctcc atggcggagc cctcggccgg gcgccacagg gcgagccgat
     9781 cgatttcctt cgccagcgcg aagtcccgct cggtgatccc gcccgacgag tgggtcatga
     9841 gcttcaccca gagcttcccc caggtgactt cgaggtcggc gtggtgatac gccgcctcgc
     9901 acaggaagcc gacggcgttg atgagcatga gcgtcgtggg ccatccgtcg gtgttgtact
     9961 tgcggcgcag ccagccgttc tccaccttcc aacccggcaa cgcggcgagc cgcgattcga
    10021 tcgcagccgc gtcgaggacc ggcagtttct cgggcatctc gaactccctt ctctcagctc
    10081 ctagaactcc cacttccc
//
LOCUS       DASRIQ010000018        16686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2614310, whole genome shotgun sequence.
ACCESSION   DASRIQ010000018 DASRIQ010000000
VERSION     DASRIQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 16686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16686
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2614310"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1957)
                     /gene="asnB"
                     /locus_tag="VFD06_00400"
     CDS             complement(44..1957)
                     /gene="asnB"
                     /locus_tag="VFD06_00400"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN02023.1"
                     /translation="MCGISGILTRGSTPVDPDRVLAMRSDLHHRGPDAVGLHAAGAVV
                     FGFNRLAIIDLSPAANQPMRADDGSVALVFNGEIYNFRELRARLEGLGRTFRTRSDTE
                     VILEGYRQWGRDLFPLLHGMFAIAVWDAPAKRLHLVRDRVGKKPLFYSDRNGEVVFAS
                     ELHALLRGLPTRPEVNPEAIALYLAYHVVPGSTCIFRGVHKVPPATCLTFDARGMDSW
                     RYWRASFSAKRRLREPEILEQLEPLVRDAVRSRLISDVPLGAFLSGGVDSSVVSAFMA
                     AEGGKVRTFTVTMPSAGYDEGPHARVVARHLGTDHSEIAIEPRTSWILPKLIWHYGEP
                     FADSSAVPSYLVSEVARREVTVVLNGDGGDESFAGYHWHLAARCADVYRGVVPEGLRR
                     MFAALGPRLGEARGLPAPTRAFGRFLVRWGNRDPRRAPWIWGDSPAPDLDGFLVPAFA
                     KTLRVRLDEVAQEAYERSDGAEELDRALALGLETYLPDDLLVKMDIASMAHSLEARSP
                     LLDHRVIEFMATVPGGAKMKRFETKRLLKALAARYVPASVLYRRKMGFSIPLAEWLRG
                     PLGPAARRVLTGRRLAARGILNADRIADLFREHTAGRDHSAILWAVLWLELWFRMFVD
                     GDLSPDQSLDEVV"
     gene            complement(2019..2840)
                     /locus_tag="VFD06_00405"
     CDS             complement(2019..2840)
                     /locus_tag="VFD06_00405"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN02024.1"
                     /translation="MRPTRDERSRELSEHFEFGRNWQDYSRFIDEGRIQQAVLDMERL
                     FGPGTLRGRSFLDIGSGSGLHSLAALRQGCASVVAVDLDPQSVEATRRTLARHAPDLV
                     WVAREDSVFAMSPETSGSFDLVYSWGVLHHTGDMWEAVRRAAMLVRGDGLLALALYIR
                     TPCCRLWKIEKWLYARSPGWVQGGIRLLFLAAQALREAASGRIPFRRTETRGMRLWND
                     AHDWLGGYPYESASPEEVERFLGSLGFRLIRSFNTAPSLGFFGSACAEYLFQRSI"
     gene            complement(2953..3855)
                     /locus_tag="VFD06_00410"
     CDS             complement(2953..3855)
                     /locus_tag="VFD06_00410"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HZN02025.1"
                     /translation="MKNILSFDVEDWQQSTLDLSLPITRRVVDNTARILDLCAAHHAR
                     GTFFVLGLVARAYPDLVRRIGAAGHEVALHGMDHAPVHVMTRDVFRADVRQSRDLVEQ
                     AAGTAVAGYRAPDFSITSESLWALQILAEEGFRYDSSLFPFAGRRYGIAESFPLPYRV
                     LCGDAALVEFPLATTELLGRRVPAAGGGYFRLFPYAWHWRAVRRINRLGSPAMTYFHP
                     YEIDADEIRLSPHRIPLRLRLSQGTGRRGLERRLARLLREFAWVPARDFIEESAASLV
                     AGRRLDLRGWPATPARWDDAPETT"
     gene            4024..5091
                     /locus_tag="VFD06_00415"
     CDS             4024..5091
                     /locus_tag="VFD06_00415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN02026.1"
                     /translation="MPIPDPAPLPRLCFVGPLQGAVHPGVVVTQGVRLSRHFREAGYP
                     VTAVSTRLNRYARLFDVAATLLRRGGRLDIVIVHTYGGPSFVVEDVASALARARGARV
                     VMLLHGGALPEFLARFPRWGRRVLRRADAIVAPSPFLARTAAAAGLQPRVIPNVIDVG
                     AYPYRERAALRPRLFWMRTYDPDYNPLMAIEVLKRVREAHPEASLMMRGQEKGMETEV
                     EALARRYGLDGALDRGGFLDPAGKVAAAEWADIFINTSRVDNMPVAVVEAAAMGMPVV
                     STAVGGVPDLLSDGTSGLLVPDGDADAMARAVLRLLAEPELALRLSRAGRAIALRSAW
                     PSVRAEWEDLFRRLPAPRTGS"
     gene            complement(5088..6452)
                     /locus_tag="VFD06_00420"
     CDS             complement(5088..6452)
                     /locus_tag="VFD06_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN02027.1"
                     /translation="MSVLHRLAYRLVQAARREPVHRVLAGMERSQWWTPEQLRADQEA
                     RFARVIRAAARTPHYARRFRDAVDPAAFRGLDDLVRLPLLEKATLRADPSALRDPGWK
                     GPLNVHVTTGSSGVPLSVFRSRDAGAHGRAAQLRGRSWFGIRQGDREVLYGGLALERL
                     GRTRARVIDRIMNRVRLDPFDLSDQVLDATLERIRVLRPAFLYGYPSALSLLAAQALR
                     RGAGRDLGLTLVQTSSERLYDHRRRLLREAFGCPVADEYGAAETSILAMECPEGGLHQ
                     ATENVFVEILDERGAPVPDGSEGEIVVTDLNNLAAPLVRYRIGDRGRLIPGACPCGRG
                     LPRMEILEGSAFGTLTLPDGRRLSGVVFYFLAESFLTRPDAGLREIVVVRRGHRLEAM
                     AVARSGGNPAHVAELGRRLEELVGTSVPIRLVDAIERRGSDKYRVLIDADRDPGGISG
                     TSDG"
     gene            complement(6449..7729)
                     /locus_tag="VFD06_00425"
     CDS             complement(6449..7729)
                     /locus_tag="VFD06_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02028.1"
                     /translation="MGGRLLYRWSDWLGGRGVVPALDAMLERQAWDAARLQADSRRRL
                     EALVAHAVRTIPFYRTRGLEGAMRLEDLPLLDRTTLREAGPALRDPGVPLEELEEATS
                     GGSTGEPAAVWLDRADGAAQTAGVLRSQSWMGLPFVVRHALLWGPPPDVVTYGTLRGR
                     LRGIAVRRTFYPTYGLTDAGAEAIRRDLSRRAYPQVIGYSSALDHVAAGAPPLPRPAR
                     AVVASAEILTPPQRRRISRFFGAKVFERYGCNEFAIVAHQCAAGGMHVLTDRVHLEIL
                     RADGRPAADGEIGEAVVTDLDNRAMPLLRYRLGDAIEAGGRCSCPLPFPVVAAVHGRM
                     ADLLVGAGGGPVSARHVAVALEAGGEALEHQVRIAGAGLTCAVRPLGSFDVARAAERL
                     HALLGRPVDVRTAPSLARWPSGKVRPVLRDGDPA"
     gene            7950..9842
                     /gene="asnB"
                     /locus_tag="VFD06_00430"
     CDS             7950..9842
                     /gene="asnB"
                     /locus_tag="VFD06_00430"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN02029.1"
                     /translation="MCAIAGYVHSDPRYVAPPGVLDAARDAMAHRGPDDLGSWSGDGA
                     GLASRRLAILDLSERGRMPMTSEDGRYVIVHNGEVYNYRELREGLKTRGHRFRSDTDT
                     EVLLRSYMERGPALLDDLNGMFAFAVWDHAERTLFVARDRLGVKPLYYAERGGAFWFA
                     SEPKALFQAGFDPRFDPGTWEELLCFRYVAGERTPYEGIRRIPPGHCLLWRDGRATLR
                     RWWSLTEAARRGAPVPGSEAPDWFAETFRSAISYRRISDVPIGVLLSGGLDSTSIACV
                     LGEQAGPGLASFTVRFPGTPLDEGKRARAAAGAFGLEHHELEIAPEQLLERLGQASWF
                     NDEPLPHGSDLHLMAISEYARPRVTVLLSGEGADETMGGYARYRPLPLLPWLGWLGPF
                     LPSAQASPALRRAAKLRAMLREDAREGPLLYNTCDVLPADLERLGMRPGRDFAHRRRV
                     LLEAREVYPDDALRQAMYTDQHAFLCSVLDVNDKMTMAASIECRVPFLDYRLVEGCAA
                     LPTRALAGLREGKRPLRRAFADRLRPVLGGYRKIGFTAPWAAYFRRTPALRAMLESLP
                     SRDPVRDGPLPETPLRRAIAGFLAGDDRPEAVLRALVMVCSWYEACVGGGRSAAGARR
                     QAGLPQ"
     gene            9839..11029
                     /locus_tag="VFD06_00435"
     CDS             9839..11029
                     /locus_tag="VFD06_00435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN02030.1"
                     /translation="MSRERRGPPADATDRPLKILHVIPTLGIGGTERQLVDLLPRMDA
                     RRYHQTVCHYTPCDSLEERLHAAGIRTVFVPKTAMPAWRFLSRLRAVVRETRPDVVHT
                     WLYSGNFWGRVAAVTCGVRRLVASDRGMALPAGWPVVLYERLLAPFTLRLVNSHAAAG
                     SVVRHYGLPSDRIRVIRNAVGPLPPVPPDARERIRAVLGLPVTERLVLLVGRQSVEKN
                     HAMFFRVARRVAAARPGVTFVAVGRLFERDAMLRRLEESGGAAHVRIVDQQPDVASWL
                     AAADLFCMTSDREGLPNALLEAMAMGLPVVCTAFESVREVVAREDVGVIVPRDDDAAM
                     AAAVLGLLDDPARCRALGGAARAHVVSNFGWERLIRDMEALYGEIDPRAARGRAPTEA
                     PGTT"
     gene            complement(11042..11773)
                     /locus_tag="VFD06_00440"
     CDS             complement(11042..11773)
                     /locus_tag="VFD06_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN02031.1"
                     /translation="MARTLLTLITALLATLVLGTVAIAACLLRPGGAWLMPLARSWSR
                     LVLAAAGVRFTATYDPAIDPRRPAVYACNHQSLFDIPVLVLAMPTDFRMVAKKSLLYI
                     PIFGWALWLAGFLFIDRSRRERAIGTLGRAAERLRRGTSIVVFAEGTRSPDGALLPFK
                     RGGFVLAIQAVVPVVPVAIRGGTSVLAKGTLALRPGVMEVAFGAPVDTTGYVYETRDR
                     LVAQVRDRIDRALAPRPPGVLPSAR"
     gene            11932..12687
                     /locus_tag="VFD06_00445"
     CDS             11932..12687
                     /locus_tag="VFD06_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02032.1"
                     /translation="MTGGATRALEQVGAFSILIARSARETVRRRPEGSVVLDQLYQLG
                     VLSVGIATVTAMFTGMVLALQTSYSLAVYGAKFFVGDIVALSLVRELGPVLTSLMVGG
                     RVGAGITAEIGTMNVTEQIDAIRAMAASPVRKLVVPKVIAIVIMLPVLVVLSDFVGIL
                     GGLLMAVTTLNQPAAFYMQHVIKAIHPEDILSGLGKTVVFALLIGFIACYKGLTATGG
                     ADGVGRATTETVVASSLSILISDFFLTKLFLAF"
     gene            12698..13447
                     /locus_tag="VFD06_00450"
     CDS             12698..13447
                     /locus_tag="VFD06_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN02033.1"
                     /translation="METPFVRFEEVHKSFGAKKVLQGITFDIRRGETMVVLGGSGSGK
                     SVLLRHIIGLHRPERGRVLVEGEDIVPYDEEELVPVRKKIGMLFQGGALFDSMDVADN
                     VGYGLREHTTLSHEEIREAVRKKLALVELSGVEHLMPSELSGGMRKRVALARSIAMEP
                     RGILYDEPTTGLDPVTAATINDLIRSMQERLQVTSVVVTHDINSALYVGDRIAFLFEG
                     RMLFVGTVDEAKQSPEPALRQFLSGRVHRGA"
     gene            13437..14600
                     /locus_tag="VFD06_00455"
     CDS             13437..14600
                     /locus_tag="VFD06_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZN02034.1"
                     /translation="MARKWARELSVGAVISVGLVVFAIAVLAITEESRLFYPKVSYWT
                     RFDNTSGLARGSPVRLVGVQIGTVDEIVFPTDLKEFKIKVTFRVDRAFAPRIRRGTVA
                     YLKSLTYLSQDKYVELTPGDPEQPQLAAGEYIDAGKSAWEETLLQGQGIADDLKEITA
                     SFRDLLVALNQGQGIVQEMIHNPEFGRQGAADLEGSLASLRRLLEGMEQGRGLAGSVL
                     RDKEMADRFKGSLDESMTHLKSILARLDEPEGALAQLSDPRGKGAALIEELRAGATSF
                     RQTAETLERGEGTIGRLLHDKQYADGLLGKIDRSVGHLDSILGKIDRGEGTLGGIVND
                     PAVYEGLRDIVAGIQKSRLGKGMIRHYGKKGAEQRQGQGEDEKEAPPDPGPNP"
     gene            14617..15612
                     /locus_tag="VFD06_00460"
     CDS             14617..15612
                     /locus_tag="VFD06_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN02035.1"
                     /translation="MRSVLVTGGAGFIGSHLVEALLQAGRDVVALDNFDSFYDPEVKR
                     RNVAAFSGRPGFRLVEGDIRDEKTVERVFTAHPVDVVVHLAARAGVRPSIEQPLLYSD
                     VNLNGTVVLLEACRRHNVGKFVFGSSSSVYGNSPKAPFSEKDDVSMPISPYAATKRAG
                     ELLCATYHALYRLNVFCLRFFTVYGPRQRPEMAIHKFTRFIDRGLPLPRFGDGSTQRD
                     YTYIDDVISGVMRAVERVQGYEILNLGGSRTTRLADLIAHLERLLDKKAVIEGQPDQP
                     GDVVTTWADVAKAHRLLEYEPKIGIEEGLARFIEWYRRTGERGAGAAPAPLGAKG"
     gene            15609..>16686
                     /locus_tag="VFD06_00465"
     CDS             15609..>16686
                     /locus_tag="VFD06_00465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZN02036.1"
                     /translation="MNIAVVGTGYVGLVTGACFAEFGVGVLGVDNDEKKIASLKAGKV
                     PFFEPGLEELVGRNMKGGRLGFTTDIKSAVEKSLVIFIAVGTPSDGEGAADLRFVREV
                     AASIAQHMNGYKVVVTKSTVPTGTGAMIRTIIQEKQRHPHPFSVASNPEFLREGAAIE
                     DFMRPNRIVIGAEDPQAIAILKDLYNPLYLIETPFVITNVVSAEMIKYASNAFLATKI
                     SFINEVAALSEAVGADVHEVARGMGLDNRIGRKFLHPGPGFGGSCFPKDTLAIQRMAR
                     EHGLPGHVVSGVIKVNDEQIPRMVEKITRAVGGLKGKTLGVLGLAFKPNTSDTRESPA
                     VRIIQEMVQAGASVRAFDPAAMEEA"
BASE COUNT         2541 a         5728 c         6010 g         2407 t
ORIGIN      
        1 ctcctgcagg aaaaagaatt tataccacag cgggcggaag gcctcagacc acctcgtcga
       61 gcgactggtc gggggagaga tcgccgtcca cgaacattcg gaaccagagc tcgagccata
      121 ggacggccca gaggatcgcg gaatgatccc ggcccgcggt gtgctcccga aacagatccg
      181 cgatgcgatc cgcgttcagg atcccgcgtg ccgcgagccg ccggcccgtg agcacgcgtc
      241 gcgcggccgg cccgagaggt ccgcggagcc actccgccag cggaatggag aaccccatct
      301 tgcggcggta gaggacggag gccggcacgt accgggcggc gagtgccttg aggaggcgct
      361 tcgtctcgaa gcgcttcatc ttcgcgccgc cgggcacggt ggccatgaat tcgatgacgc
      421 ggtgatccag gagcggcgag cgcgcctcga gggaatgggc catcgaggcg atgtccatct
      481 tgacgaggag atcgtcggga agataggtct ccaggcccag ggccagcgcc cgatcgagtt
      541 cctcagcgcc atccgacctc tcatacgcct cctgcgccac ttcatcgagc cgcacgcgca
      601 gcgtcttcgc gaaggccgga acaaggaatc cgtccagatc gggtgccggg ctgtcgcccc
      661 agatccacgg cgcgcgccgc gggtcgcggt tgccccaccg gacgagaaaa cgaccgaagg
      721 cgcgcgtcgg agcgggcaac cccctcgctt cgccgaggcg ggggccgagc gcagcgaaca
      781 tccgccgcag gccctccgga acgacgccgc ggtacacatc ggcgcagcgg gccgccaggt
      841 gccagtggta tccggcgaag ctctcgtccc cgccgtctcc gttgagcacg acggtcacct
      901 cgcggcgcgc gacctcgctc acgaggtagc tcgggacggc tgaggaatcc gcgaacggct
      961 ctccgtaatg ccaaatcagc ttcggaagga tccaggacgt tctcggctcg atcgcgattt
     1021 cgctgtgatc ggtccccagg tgccgggcca ccacgcgcgc gtgaggcccc tcgtcgtatc
     1081 ccgccgaggg cattgtgacc gtgaatgtcc gcaccttccc gccttcggcg gccatgaagg
     1141 cgctcaccac gctcgagtcg actccgccgc tgagaaaggc gccaagcggg acatcgctga
     1201 tcaggcggga tcggacggcg tcgcggacga gcggctccag ctgctcgaga atctccggtt
     1261 cgcgaaggcg gcgcttggcc gagaacgagg cccgccagta tcgccaggag tccatgccac
     1321 gcgcatcgaa ggtcaggcag gtggccggag ggaccttgtg cactccgcgg aagatgcagg
     1381 tcgatcccgg gaccacgtga taggcgaggt agagagcaat cgcctcggga ttgacctccg
     1441 gtcgcgtcgg aagcccgcgc aggagggcat ggagctcgga cgcaaacacg acctccccgt
     1501 tccggtcgga atagaagagc ggcttcttgc cgacccgatc gcggaccagg tgcaggcgct
     1561 tcgcgggggc gtcccagacg gcgatcgcga acattccgtg cagcaagggg aagagatcgc
     1621 gtccccactg tcgatagcct tccaggatga cttcggtgtc gctccgcgtc cggaacgtcc
     1681 gcccgagccc ctccaggcgg gcgcggagct cgcggaaatt gtaaatctcg ccgttgaaga
     1741 cgagcgccac cgacccgtcg tcggcacgca tcggctgatt cgcggccggc gacaagtcga
     1801 taatcgccag ccggttgaag ccaaagacga ccgcgcctgc cgcgtggagt cccacggcgt
     1861 ccggtccacg gtggtgcagg tcggatcgca tcgccaggac ccgatcggga tccacgggag
     1921 tcgatccccg cgtcaggatt ccggagatgc cgcacatgac cgccattcta gcggagcggc
     1981 cgtgtctgcc tgattttcga gaccgccgtc gggcgtgcct agatggatcg ctggaagagg
     2041 tattcggcgc aggcggaccc gaaaaacccg agcgagggag cggtgttgaa gctccggatc
     2101 aggcggaaac cgaggctccc caggaaacgc tccacctctt ccggcgacgc cgattcgtag
     2161 ggatatccac ccagccagtc gtgcgcgtcg ttccagagcc gcattccccg ggtctcggtt
     2221 cgccggaatg gaatgcgccc ggacgccgcc tcgcgcaggg cctgagcggc caggaaaagg
     2281 agacggatcc ccccctggac ccagccggga ctgcgtgcat agagccactt ctcgatcttc
     2341 cagagccggc agcaaggggt cctgatgtag agggccagag ccaacaggcc atcgcccctg
     2401 acgagcatcg ccgcccgtcg gaccgcctcc cacatgtccc cggtgtgatg caggacgccc
     2461 cagctgtaga cgaggtcgaa gctcccgctc gtttcgggcg acattgcaaa gacactgtcc
     2521 tccctggcga cccagaccag gtcgggagcg tgccgcgcca gcgtcctccg ggtcgcctcg
     2581 acgctctggg gatccagatc gaccgcgacg accgacgcgc agccctgccg gagcgcagcg
     2641 agcgagtgca ggccggaccc cgaaccgatg tccaggaacg atctgcctct cagcgtccct
     2701 ggtccgaaca gccgctccat gtcgagcacc gcctgctgaa ttcggccctc atcgataaat
     2761 cgagagtaat cctgccagtt ccggccgaac tcgaaatgct ccgacagctc gcgggagcgc
     2821 tcgtctctcg tgggtcgcat cctgatcacc attatccgat tcgggtgacc gccccgggca
     2881 agaggacctc tcgatgatcc ggcttccggc cggcgtttct cgcggcgggc gcgcgcgccg
     2941 aacccaatgg attcacgtgg tctccggcgc gtcatcccag cgcgccggtg tcgcgggcca
     3001 gccccgcagg tcgagccggc gcccggccac caggctcgcg gcactctcct cgatgaaatc
     3061 gcgcgccggg acccaggcga actcgcgcag aaggcgagcc agccgccgct cgagcccgcg
     3121 gcggccggtt ccctgcgaga gcctgagtcg gagcgggatc cggtgcggag aaaggcgaat
     3181 ttcgtccgcg tcaatctcgt acggatggaa ataggtcatc gcagggcttc cgagccggtt
     3241 gatccggcgc accgccctcc agtgccaggc gtaagggaac aggcggaaat acccgccgcc
     3301 tgcggcgggg acccgtcggc cgagcagctc cgtcgtcgcc agcgggaact cgaccagcgc
     3361 ggcgtcgccg cagaggacgc ggtaagggag cgggaacgac tccgcgatcc catagcgccg
     3421 cccggcgaac gggaacaggc tcgagtcgta gcgaaacccc tcctccgcca ggatctggag
     3481 ggcccagagc gactcgctcg tgatggagaa atcgggggcg cggtatccgg cgacggcggt
     3541 cccggccgcc tgctcgacaa ggtccctcga ctgccgcacg tcggcgcgga agacgtcgcg
     3601 cgtcatgacg tggaccggag cgtggtccat tccgtgcagc gcgacctcgt ggccggcagc
     3661 cccgatccgc cggaccaggt cgggataggc gcgcgccacc agcccgagta cgaagaaggt
     3721 gccgcgtgcg tggtgggcgg cgcacaaatc gaggatgcgc gccgtgttgt cgacgacccg
     3781 gcgggtgatc ggaagcgaca gatcgagcgt cgactgctgc cagtcctcga cgtcgaagga
     3841 caggatgttc ttcagggggc cctcggtcgc ccgcgtcccg gcggggcgcg cggcccggac
     3901 aggcccgcgc gcggagtatg gcctgtcgcc gcgcggcgat caaccgaagt tcgcggatac
     3961 aatgcgcgcc ggcgcccgaa ccgacgatga cgtcgcgagg ctgatggaat ttcaccgctg
     4021 accgtgccca tcccggatcc ggctccgctc ccgaggctgt gcttcgtcgg tccgctccag
     4081 ggtgcggtcc atcccggcgt ggtggtgact cagggggtgc gcctgtcgcg gcatttccgt
     4141 gaggccggct acccggtcac cgccgtgtcc acccgcctga accggtacgc gaggcttttc
     4201 gacgtcgccg cgacgctcct ccgccgcggt ggtcgcctcg acatcgtcat cgtgcacacg
     4261 tacggaggac cgagcttcgt ggtcgaggac gtcgccagcg ccctggcgcg ggcgcgcggc
     4321 gcgcgcgtcg tcatgctcct gcacggaggg gcgctgccgg agttcctcgc ccgcttcccg
     4381 cggtgggggc gccgggtgct gcgccgcgcc gacgccatcg tcgcgccgtc cccgtttctg
     4441 gcccgcaccg ccgccgcggc cggcttgcag ccgcgcgtca tcccgaacgt gatcgacgtc
     4501 ggcgcctatc cctaccgcga gcgcgcggcg ctgcgtccgc ggttgttctg gatgcgcacc
     4561 tacgatcccg actacaaccc gctgatggcg atcgaggtcc tgaagcgggt ccgcgaggcg
     4621 catcccgaag cctccctgat gatgcgcggg caggagaagg gcatggagac ggaggtggaa
     4681 gcgctggcgc ggcggtacgg gctcgatggg gcgctcgatc ggggagggtt cctcgatccg
     4741 gccggcaagg tcgccgccgc ggaatgggcc gatatcttca tcaacacgag ccgcgtggac
     4801 aacatgccgg tggcggtcgt cgaggccgcc gcgatgggga tgccggtcgt cagcaccgcg
     4861 gtcggaggcg tgcccgatct cctgagcgac gggacctcgg ggctcctggt tccggacggc
     4921 gacgcggacg ccatggctcg ggcggtgctc cgcctgctcg cagagccgga gctcgccctg
     4981 cggctctcgc gcgcggggcg ggcgatcgcc ctgcgatcgg cgtggccgtc cgtgcgggcc
     5041 gaatgggagg acctgttccg ccgcctcccc gcgccgcgga caggctccta gccatcggac
     5101 gttccggaga tcccgccggg gtcccggtcg gcgtcgatca ggacccggta cttgtccgat
     5161 ccccggcgct cgatggcgtc caccagccgg atcggcacgc tcgtgccgac cagctcctcg
     5221 aggcgccggc cgagttccgc gacgtgcgcc gggtttcccc ccgagcgcgc caccgccatc
     5281 gcttcgaggc gatggccgcg tcgcacgacg acaatctcgc gcagccccgc gtccggccgg
     5341 gtgaggaacg actcggcgag gaagtagaag acgacgccgc tcagccgccg tccgtcaggc
     5401 agggtcaggg ttccgaaggc gctcccctcg aggatttcca tgcggggcag accgcgcccg
     5461 caggggcagg cgccggggat gaggcggccg cggtcgccga tgcggtagcg cacgagcggc
     5521 gccgcgaggt tgttgagatc ggtcaccacg atctcacctt cggacccatc gggcacgggc
     5581 gccccgcgtt cgtcgagaat ctcgacgaag acgttctcgg tcgcctggtg cagtcctcct
     5641 tccgggcact ccatggccag gatggaggtc tcggcggcgc cgtattcgtc ggccacggga
     5701 cagccgaacg cctcccggag gaggcggcgg cgatgatcgt agaggcgctc cgaggaggtc
     5761 tggacgagag tcagcccgag gtcgcggccg gcgccgcgtc gcagcgcctg cgccgccaga
     5821 agcgacaggg cggaaggata accgtacagg aaggccggcc gcagcacgcg aatgcgttcg
     5881 agcgtggcgt ccaggacctg gtcggagaga tcgaacggat cgagacgcac acggttcatg
     5941 atccggtcga tcacccgggc gcgcgtccga ccgaggcgct ccagggcgag cccgccgtag
     6001 agaacctcgc ggtcgccctg gcggatgccg aaccaggacc gcccgcgcag ctgcgcagcg
     6061 cgtccgtgcg cgcccgcgtc ccggctccga aagacgctca gcggcacgcc ggaggagccg
     6121 gtggtgacgt gcacgttcag ggggcccttc cagccagggt cccgcagcgc agagggatcg
     6181 gcgcggagcg tcgccttctc gaggagcggc aggcggacga ggtcgtccag accgcggaac
     6241 gcggccgggt cgacggcgtc gcggaagcgt cgcgcgtaat gcggcgtccg ggcggccgcc
     6301 cggatcacgc gcgcgaaacg cgcctcctga tcggcacgca gctgttccgg cgtccaccac
     6361 tggctccgct ccatcccggc gaggacgcgg tgcaccggtt cgcgccgggc cgcctggacg
     6421 aggcggtagg ccaggcggtg caggacgctc atgccggatc gccgtcgcgc aggacggggc
     6481 gcaccttgcc ggacggccag cgcgcgagcg agggggcggt ccggacatcg accggccgtc
     6541 cgagcagggc gtgcaggcgt tccgcggcgc gcgcgacgtc gaaggagccg agaggacgga
     6601 ccgcacacgt gagccccgct ccggcgatgc gcacctggtg ctcgagcgct tcgccgcccg
     6661 cctcgagggc gacggcgacg tgtcgggccg agaccggccc gccaccggcc ccgaccagga
     6721 gatcggccat ccggccgtgc acggcggcga ccaccgggaa ggggagcggg caggagcatc
     6781 gtccgccggc ttcgatggcg tcgccgagcc gatagcgcag aagcggcatg gcgcggttgt
     6841 ccagatcggt gacgaccgcc tccccgattt cgccgtcggc cgccggccgg ccgtcggccc
     6901 gcagaatctc gaggtgaacg cggtcggtca gcacgtgcat cccgcccgcc gcgcactggt
     6961 gcgccacgat cgcgaactcg ttgcatccgt agcgctcgaa caccttggct ccgaagaacc
     7021 ggctgatgcg ccggcgctgc ggcggagtga gaatctccgc agaggccacg accgcgcgcg
     7081 ccggacgcgg gagcggtggg gcgccggcgg cgacgtgatc gagcgcgctg ctgtacccga
     7141 tgacctgggg gtaggcgcgg cggctcaggt cgcggcggat cgcttcggcc ccagcgtcgg
     7201 tcagcccgta ggtgggatag aacgtccggc gcaccgcgat ccctcgcagc ctcccgcgca
     7261 gcgtgccgta ggtgacgaca tccggcggag gaccccacag gagggcgtgc cggacgacga
     7321 agggaagacc catccaggat tgggagcgga ggacccccgc cgtctgcgcg gcgccgtcgg
     7381 cccggtcgag ccagacggcc gccggttccc cggtgctgcc gccgctcgtg gcttcctcga
     7441 gctcctcgag agggacaccc ggatcccgca gcgcgggccc ggcctcgcgc agcgtcgtgc
     7501 gatccaggag cggcaggtcc tcgaggcgca tcgcaccctc gaggccgcgc gtccggtaga
     7561 aggggatcgt ccgcaccgcg tgcgccacga gggcctcgag cctgcggcgg gaatcggcct
     7621 gcagccgcgc ggcgtcccag gcctgtcgct cgagcattgc gtcgagggcc ggcacgacgc
     7681 cccggccgcc gagccagtcc gaccatcgat agaggagtcg ccctcccaac cgcgccctcc
     7741 ggacggcggt ctggcgccgc cgcgaagcgg cgacgatacg gtcggccgcg gcgactgtca
     7801 atctgctaga ctccgctgtg agcgcgcgat ccggctcctg ccgaccgcgg gtcgcccgcc
     7861 ctggaatccc tgaaccctcg agtgtcccgt ccggcttccc atcgtttcct ggcgtcgccg
     7921 ccgcccggcg ccgcggcagg ggcccgctga tgtgcgccat tgccgggtac gtccactccg
     7981 atccccgcta cgttgcgcca ccgggggtgc tcgacgccgc ccgggacgcg atggcccacc
     8041 ggggccccga cgatctcggc tcatggtcgg gagacggcgc cgggctggct tcccgcaggc
     8101 tggcgatcct ggatctctcg gagcggggcc ggatgccgat gacgagcgag gacggccgct
     8161 acgtcatcgt ccacaacggc gaggtttaca actaccgcga gctgcgcgag gggctgaaga
     8221 ctcggggcca ccgcttccgg tccgacacgg acaccgaggt gctgctgcgc tcgtacatgg
     8281 agcgcgggcc cgccctgctc gacgatctca acggcatgtt cgcgttcgcg gtctgggacc
     8341 acgcggagcg caccctgttc gtcgcgcgcg accggctggg ggtcaagccg ctctactacg
     8401 cggagcgcgg cggcgctttc tggttcgcct cggagcccaa ggcgctcttc caggccgggt
     8461 tcgacccgcg cttcgatccc gggacgtggg aggaactgct ctgcttccgc tacgtggccg
     8521 gggagcgtac cccctacgag gggatccgac ggatcccacc cggtcactgt ctgctctggc
     8581 gggacgggcg cgcgacgctg cggcgctggt ggtccctgac ggaggcggct cgccggggcg
     8641 ctccggtccc cgggtcggag gcgccggact ggttcgcgga gacctttcgg agcgcgatct
     8701 cctaccggcg catcagcgac gtcccgatcg gcgtgctcct gagcggcggc ctggactcga
     8761 cgagcatcgc ctgcgtgctc ggcgagcagg ccggccccgg cctggcctcc ttcaccgtgc
     8821 ggtttcccgg cacgccgctc gacgaaggga agcgggcgcg tgccgcggcg ggcgccttcg
     8881 gcctcgagca ccacgagctg gagatcgcgc cggagcaact cctcgagcgg ctcggacagg
     8941 cctcctggtt caacgacgag ccgcttccgc acggcagcga cctccatctc atggcgatct
     9001 cggaatacgc ccgcccccgg gtcaccgtgc tgctctccgg cgagggggcc gacgagacga
     9061 tgggcggcta cgcccgttac cgtccgctgc cgctgctccc gtggcttggg tggctcggtc
     9121 cgttcctgcc ctcggcgcag gcgtcgccgg cgctccgccg cgcggcgaag ctgcgcgcga
     9181 tgctgcggga ggacgcgcgc gaggggccgc tcctctacaa cacctgcgac gtgctgccgg
     9241 cggatctcga aaggctcggg atgcggcccg gccgcgattt cgcccaccgc cgccgggtcc
     9301 tgctggaggc gcgggaggtc tatccggacg acgcgctgcg gcaggcgatg tacacggatc
     9361 aacacgcctt cctgtgctcg gtgctcgacg tcaatgacaa gatgaccatg gcggcctcga
     9421 tcgaatgccg cgtgccgttc ctcgactacc gcctggtcga gggttgcgcg gcgctgccga
     9481 cccgggcgct cgcggggctg cgggagggga aacggccgct gcgccgcgcg ttcgcggatc
     9541 ggctccggcc ggtcctcgga ggctatcgca agatcggctt caccgccccg tgggccgcct
     9601 acttccgccg gacgccggcg ctccgggcga tgctcgagtc gctgccgtcg cgcgacccgg
     9661 tgcgcgacgg cccgcttccg gaaacgcctc tgcgtcgggc gatcgccggg ttcctcgccg
     9721 gggacgaccg gcccgaggcg gtgctccgcg cgctcgtgat ggtttgctcg tggtacgagg
     9781 cgtgcgtcgg cggcggccgg tccgccgccg gagcccggcg acaggccggc ctccctcagt
     9841 gagccgggag aggcgcggcc cgccggcgga cgccaccgac cgcccgctga agatcctcca
     9901 cgtcatcccg accctgggaa tcggcgggac ggagcggcag ctggtcgatc tcctcccgcg
     9961 gatggacgcg cgacgctacc accagacggt gtgtcactac acgccgtgcg actcgctcga
    10021 ggagcggctc cacgccgcgg ggatccggac cgtgttcgtc cccaagacgg cgatgccggc
    10081 ctggcgtttc ctgtcccgtc tgcgggccgt ggtgcgggag acgcggccgg acgtggttca
    10141 cacgtggctc tactcgggga atttctgggg ccgcgtggcg gccgtgacct gcggcgtgcg
    10201 gcggctggtg gcgagcgacc gcggcatggc cctgccggcc ggctggcccg tggtcctgta
    10261 cgagcggctg ctggcgccgt tcacgctgcg actggtgaac agccacgcgg ccgccggttc
    10321 cgtggtgcgg cactacggcc ttccctcgga tcgcatccgg gtcatccgca acgcggtcgg
    10381 cccgttgccg cccgtcccgc cggacgctcg ggagcgaatc cgcgccgtgc tcggcctgcc
    10441 ggtcaccgag cgcctcgtcc tcctcgtcgg gcggcagtcg gtcgagaaga accacgcgat
    10501 gttcttccgg gtggcgcgcc gtgtcgcggc ggcgcggccc ggcgtcacct tcgtggccgt
    10561 ggggcgcctg ttcgagcggg acgcgatgct gcgccggctc gaagagtcgg gaggggcggc
    10621 gcacgtccgc atcgtcgatc agcagcccga cgtcgcgtcc tggctcgccg ccgcggatct
    10681 gttctgcatg acgtcggatc gcgaagggct gccgaacgct ctgctcgagg cgatggccat
    10741 gggactcccg gtcgtctgca ccgccttcga gtcggtccgg gaggtggtgg cgcgcgagga
    10801 cgtgggggtg atcgtgccgc gcgatgacga cgccgccatg gcggcggccg tgctcggtct
    10861 tctcgacgat ccggcccggt gccgcgccct gggcggggcg gcgcgggcgc acgtcgtatc
    10921 aaactttggt tgggagcggc tgattcgcga catggaggcg ctctacgggg agatcgaccc
    10981 gcgtgccgcg cggggccgcg cgccgacgga ggcgccgggg acgacctgaa tgcggcctcc
    11041 gctaccgggc cgacgggagg actccgggcg gccgcggggc gagcgcccgg tcgatccgat
    11101 cgcgcacctg cgcgaccaga cgatcgcgcg tctcgtagac gtagccggtc gtgtccacgg
    11161 gcgccccgaa ggcgacctcc atgacgccgg gcctcagggc gagggttccc ttggccagaa
    11221 ccgaggtgcc gccccggatc gccacaggga cgaccgggac gacggcctgg atggcgagca
    11281 cgaagccgcc ccgcttgaac ggcaggagcg ccccgtccgg actgcgcgtg ccttccgcga
    11341 acacgacgat cgacgtgccg cgccggaggc gctcggcggc gcgtccgagc gtgccgatgg
    11401 cccgctcgcg gcgggaccga tcgatgaaca ggaacccggc gagccagaga gcccagccga
    11461 agatcgggat gtagaggagc gatttcttgg cgaccatccg aaaatcggtc ggcatggcga
    11521 ggaccaggac cggaatgtcg aagaggctct ggtgattgca ggcgtagacc gccggccgac
    11581 gcggatcgat cgccgggtcg tacgtggccg taaagcgaac ccccgccgcg gcaaggacca
    11641 gccgggacca gcttcgcgcc agcggcatca gccacgcccc gcccggccgc agcaggcagg
    11701 ctgcgatcgc caccgtcccg agaacgaggg tggccagaag ggccgtgatc agggtgagca
    11761 gtgtgcgcgc catcgtccgt ccgcgggccc tccccggcgg ccgcccgcgc atcataagtc
    11821 catgcagccg cggtcggatt ccgtgaggct tccggttgcg gtagactccc gccgaactat
    11881 ggcgcggaag gggccccgag cgggccggcg tgaaccgggg atcggccggc gatgaccggg
    11941 ggcgcgacgc gcgctctcga acaggtcggt gccttctcca tcctgatcgc gcgctccgcg
    12001 cgcgagacgg tccggcggcg gccggaagga tccgtcgtcc tcgaccagct gtaccagctc
    12061 ggcgtcctct cggtcggaat cgccacggtc accgccatgt tcaccgggat ggtgctggcg
    12121 ctgcagacct cctattcgct cgccgtctac ggggcgaagt tcttcgtggg cgacatcgtc
    12181 gccctgtcgc tggtccgcga gctgggtccg gtgctgacct ccctgatggt tggggggcgc
    12241 gtcggggccg gcatcacggc cgagatcggc acgatgaacg tcacggagca gatcgacgcg
    12301 atccgggcca tggccgcgag tccggtccgc aagctggtcg tccccaaggt cattgccatc
    12361 gtcatcatgc tgccggtgct ggtggtgctc tccgatttcg tcggcatcct ggggggcctg
    12421 ctgatggcgg tgacgacgct gaaccagccg gcggccttct acatgcagca cgtcatcaag
    12481 gcgatccacc cggaggacat cctcagcggc ctcggcaaga ccgtggtctt cgccctgctc
    12541 atcggcttca tcgcgtgcta caagggtctg accgccaccg gcggggccga cggcgtcggg
    12601 cgggcgacga ctgagacggt ggtggcgtcc tcgctcagca tcctcatcag cgacttcttc
    12661 ctgaccaagc tgttcctggc gttctgacgt tgctccgatg gagacgccgt tcgtccggtt
    12721 cgaggaggtc cacaagagct tcggcgcgaa gaaggtgctg caggggatca ccttcgacat
    12781 ccgccggggc gagacgatgg tcgtcctcgg cggcagcggc agcggcaagt cggtcctcct
    12841 gcgccatatc atcgggctgc accgtcccga gcgcgggcgg gtgctggtcg agggggagga
    12901 catcgtcccg tacgacgagg aggagctcgt gccggtgcgc aagaagatcg gcatgctgtt
    12961 ccagggggga gcgctgttcg actcgatgga cgtcgcggac aacgttggct acggcctgcg
    13021 cgagcacacg accctgtcgc acgaggagat ccgggaggcc gtgaggaaga agctggcgct
    13081 ggtggagctt tcgggggtcg agcacctgat gccctccgag ctgtcgggag ggatgcgcaa
    13141 gcgcgtggcc ctcgcccgct ccatcgccat ggagccgcgc ggcatcctct acgatgagcc
    13201 gaccaccggc ctcgacccgg tgaccgccgc gaccatcaac gacctcatcc gcagcatgca
    13261 ggagcgcctc caggtgacct cggtcgtggt gacgcacgac atcaacagtg ccctgtacgt
    13321 cggcgaccgc atcgccttcc tcttcgaggg acgcatgctg ttcgtcggga ccgtcgacga
    13381 ggcgaagcag agccccgagc cggcgctgcg ccagttcctg tccgggaggg ttcaccgtgg
    13441 cgcgtaagtg ggcccgcgag ctctccgtcg gcgcggtgat ctccgtgggg ctggtcgtct
    13501 tcgcgatcgc cgtgctggcg atcaccgagg agtcgcgcct gttctatccc aaggtgtcct
    13561 actggacccg cttcgacaac acttcggggc tcgcgcgcgg ctccccggtg cggctggtcg
    13621 gggtgcagat cggcacggtg gacgagatcg tcttcccgac cgatctcaag gagttcaaga
    13681 tcaaggtcac cttccgcgtc gatcgcgcct tcgccccgcg catccgccgc gggacggtgg
    13741 cctacctgaa atcgctgacc tatctttcgc aggacaaata tgtcgagctg acaccggggg
    13801 acccggagca gccgcagctg gcggcggggg agtacatcga cgcgggcaag tccgcctggg
    13861 aggagacgct cctgcagggg cagggaatcg ccgacgacct gaaggagatc accgcgtcgt
    13921 tccgcgacct gctggtggcg ctgaaccagg ggcaggggat cgtgcaggag atgatccaca
    13981 atcccgaatt cggacggcag ggggccgccg acctggaggg gagcctggcg tcgctgcgcc
    14041 ggctcctcga ggggatggag caggggcgcg ggctggcggg gtcggtgctg cgcgacaagg
    14101 agatggcgga tcgtttcaag ggatccctcg acgagtcgat gacgcatttg aaatcgatcc
    14161 tggcgcgact ggacgagccg gagggggccc tggcgcagct cagcgatccc cgggggaagg
    14221 gggcggccct catcgaggag ctgcgggccg gggcgacctc cttccgacag acggccgaga
    14281 cgctcgagcg cggcgagggg accatcggcc ggctgctgca cgacaagcag tacgccgacg
    14341 ggctgctggg gaagatcgac cgcagcgtcg ggcatctcga ctcgatcctc ggcaagatcg
    14401 accgcggcga agggacgctt ggcggaatcg tcaacgaccc tgccgtgtac gaggggctgc
    14461 gcgacatcgt ggcggggatc cagaagagcc ggctcggcaa gggcatgatc cggcattacg
    14521 gcaagaaggg ggccgagcag cggcaggggc agggcgagga cgagaaagag gccccgccgg
    14581 atccggggcc gaatccgtag gaaggcggcg acggcgatgc gcagcgtgtt ggtgacggga
    14641 ggggcggggt tcatcggctc gcacctggtg gaggcgttgc tccaggccgg gcgggacgtc
    14701 gtcgccctcg acaacttcga cagcttctac gatccggagg tcaagcgccg caacgtcgcg
    14761 gccttctctg gccggcccgg cttccgcctc gtcgaggggg acatccgcga cgagaagacg
    14821 gtcgagcgcg tcttcaccgc gcacccggtg gacgtggtgg tgcacctcgc cgcgcgcgcc
    14881 ggcgtccggc cgtcgatcga gcagccgctg ctctacagcg acgtcaacct gaacgggacg
    14941 gtcgtactgc tcgaggcctg ccgccgtcac aacgtcggga agttcgtctt cggctcctcc
    15001 tcttcggtgt acgggaactc gccgaaggcc cccttctcgg agaaggacga cgtgtccatg
    15061 ccgatctcgc cgtatgcggc gaccaagcgc gccggcgagc tcctgtgcgc gacgtaccac
    15121 gccctgtacc ggctgaacgt cttctgcctg cggttcttca ccgtgtacgg tccgcgacag
    15181 cgcccggaga tggcgatcca caagttcacg cgcttcatcg atcgcggcct gccgctgccg
    15241 cgcttcgggg acggatcgac gcagcgcgac tacacctaca tcgacgacgt catctcaggc
    15301 gtgatgcgcg ccgtcgagcg ggtgcagggt tacgagatcc tgaacctcgg cggatcgcgc
    15361 accacccgcc tggcggacct gatcgcccat ctggagcggc ttctggacaa gaaggcggtg
    15421 atcgaggggc agccggatca gccgggggac gtcgtgacca cctgggccga cgtcgccaag
    15481 gcgcacaggc tcctggaata cgagccgaaa atcggcatcg aggaaggcct cgcccggttc
    15541 atcgagtggt accggcggac gggggagcgt ggagcgggcg ccgcgccggc gcctttggga
    15601 gcgaagggat gaacattgcg gtggtgggaa cgggatacgt cggcctggtc accggcgcct
    15661 gcttcgccga gttcggcgtc ggcgtgctcg gcgtggacaa tgacgagaag aagatcgcca
    15721 gcctgaaggc cgggaaggtg ccgttcttcg agccggggct cgaggagctg gtggggcgca
    15781 acatgaaggg ggggcgtctc gggttcacga ccgacatcaa gtcggcggtg gagaagtccc
    15841 tggtgatctt catcgcggtc gggaccccct cggacggcga gggcgccgcc gacctgcgct
    15901 tcgtgcgcga ggtcgcggcg agcatcgcgc agcacatgaa cggctacaag gtcgtggtca
    15961 ccaagagcac cgtcccgacc ggcaccggcg cgatgatccg gacgatcatc caggagaagc
    16021 agcgccaccc gcacccgttc agcgtggcga gcaaccccga gttcctgcgc gagggggccg
    16081 cgatcgagga cttcatgcgc ccgaaccgca tcgtcatcgg cgccgaggat ccgcaggcga
    16141 tcgccatcct caaggatctc tacaatccgc tctacctgat cgagaccccg ttcgtgatca
    16201 ccaacgtcgt cagcgccgag atgatcaaat acgcctccaa cgccttcctg gcgaccaaga
    16261 tctccttcat caacgaggtg gcggcgctgt ccgaggcggt gggagccgac gtgcacgagg
    16321 tggcccgcgg catgggtctc gacaaccgca tcgggcgcaa gttcctgcat cccggccccg
    16381 gcttcggtgg ctcctgcttc ccgaaggaca cgctggcgat ccagaggatg gcgcgcgagc
    16441 acggcctgcc cggccacgtg gtctcgggcg tcatcaaggt gaacgacgag cagatcccgc
    16501 gcatggtgga gaagatcacc cgggcggtgg gcggcctgaa ggggaagacg ctcggggtcc
    16561 tggggctggc gttcaaaccg aacaccagcg acacgcgcga gagcccggcg gtgcgcatca
    16621 tccaggagat ggtccaggcg ggcgcgtcgg tgcgcgcctt cgacccggcc gccatggagg
    16681 aggcga
//
LOCUS       DASRIQ010000019         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1828852, whole genome shotgun sequence.
ACCESSION   DASRIQ010000019 DASRIQ010000000
VERSION     DASRIQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828852"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..729
                     /locus_tag="VFD06_00470"
     CDS             106..729
                     /locus_tag="VFD06_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02037.1"
                     /translation="MPPLANTISPTLVTIPGFGLLALHVALTLSVLAALIERPFVTRA
                     GVSRFALCASLQANFITTLVGLLCTPIAFFVLAFPALLLVWWPLAVWLSYRVECWWYR
                     RSDPAVTLNRLPVLWGNLASNLVFFVLLAVTPHPSPSRLRWLHSLQPELTILTGAACA
                     ATYVAAFVIPMVFRNRGCPNPASIAPAAEQAAPVAASEVPIEAALRT"
     gene            complement(718..1086)
                     /locus_tag="VFD06_00475"
     CDS             complement(718..1086)
                     /locus_tag="VFD06_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02038.1"
                     /translation="MAGVPLSAATRERLERLFHGEDARVAAELLVNECGGNLPFAANV
                     DPVGCERTRFAALKLSKGRLDLLRQAIELAKVDWRDLLMAAGFGHDVNAHKGWVPERR
                     AGNGEKNPPRIDAAEPGSGS"
     gene            complement(1100..1534)
                     /locus_tag="VFD06_00480"
     CDS             complement(1100..1534)
                     /locus_tag="VFD06_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02039.1"
                     /translation="MATTDALAADLRSFTPTGNEYDDVEKLRGLITQWAGEPEAARAA
                     LLPLVFGIFERNPDNAEMGIPGPVVHALEEVGGYEQELAQSLRRMPSHYGLWMANRIL
                     NALEDERERAPWMDLLRQAAKDARISSELRDEAGEFLEFQEE"
     gene            complement(1554..1865)
                     /locus_tag="VFD06_00485"
     CDS             complement(1554..1865)
                     /locus_tag="VFD06_00485"
                     /inference="COORDINATES: protein motif:HMM:NF042844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6794 domain-containing protein"
                     /protein_id="HZN02040.1"
                     /translation="MTLGRKHRKPVDFMPKTVAEAVDRLVAGLSEDERRRLRDMAKED
                     LILLHHTWGLGIRNKFGLWGENEALVESLPPEHRFADDASMFLIEAAWERLRGGGSSD
                     R"
     gene            complement(1899..>2228)
                     /locus_tag="VFD06_00490"
     CDS             complement(1899..>2228)
                     /locus_tag="VFD06_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZN02041.1"
                     /translation="ARIGPHGLLVGLVGIAGSTKLGQYVTLGGQVGIAGHLEVGDRVT
                     VAAQSGVINDVEPGVTLIGSPAMPAAQARRVYTVFTQLPELLERVKKLEQLLAGGGER
                     AGGEDRG"
BASE COUNT          297 a          899 c          629 g          403 t
ORIGIN      
        1 atcacgtgat gtgtctcagc ccaatcggcc ctgtcgaccg cggcccacct ccgagatttc
       61 gcttccgaat ccccccgccc cgcggcgata atcccggcaa cagccgtgcc ccccctcgcc
      121 aacaccatct cccccacgct cgtcaccatt cccggcttcg gcttgctcgc cctccacgtc
      181 gctttgacgc tctccgtcct ggccgcgctt atcgagcggc cgttcgtcac ccgcgccggc
      241 gtctcgcgct tcgccctctg cgcgtcgctc caggcgaact tcatcacgac gctcgtgggg
      301 ctgctctgca cgcccatcgc gttcttcgtg ctggcgttcc ccgcgctgct cctcgtctgg
      361 tggccgctcg cggtgtggct gtcgtaccgg gtggaatgct ggtggtaccg ccgcagcgac
      421 ccggcggtga cgttgaaccg cctcccggtc ctctggggca acctagccag caacctcgtc
      481 ttcttcgtgc tgctggcggt tacgccgcac ccgtccccgt cgcgcctcag gtggctgcac
      541 tcgctgcaac cggagctgac gatcctgacc ggcgccgcct gcgcggcgac atacgttgcg
      601 gccttcgtaa tcccgatggt atttcgcaac cggggctgtc cgaaccccgc ttcgatcgct
      661 cccgcagccg aacaggctgc accggttgcc gcttcggaag ttccgatcga agccgcccta
      721 cgaacctgat ccgggttcag ccgcgtcgat ccgcggcgga ttcttttcgc cgttaccggc
      781 ccgccgctcc ggcacccacc ccttgtgcgc gttgacgtcg tggccaaacc ccgccgccat
      841 cagcaggtcg cgccagtcga ccttcgcgag ctcgatcgcc tgccgcagga ggtcgagccg
      901 ccccttgctc agcttgagcg cggcgaaccg ggtccgctcg cacccaaccg ggtcaacgtt
      961 cgcggcgaac ggcaggttgc cgccgcactc gttcaccagc agctcggcgg cgacgcgcgc
     1021 gtcctcgccg tggaacaacc tctccagccg ctcccgcgtc gccgcgctca ggggaactcc
     1081 cgccattaca gcctcctcgt cactcctcct gaaactcaag gaactccccg gcctcgtccc
     1141 tgagctctga cgatatgcgg gcgtcctttg cagcctggcg cagcaggtcc atccatggcg
     1201 cccgctcccg ctcgtcctcg agcgcgttga ggatccggtt cgccatccag aggccgtagt
     1261 gcgacggcat ccgcctcagc gactgcgcca gctcctgctc gtatcccccc acctcttcca
     1321 gcgcgtgcac gacggggccg ggaatgccca tctccgcgtt gtcggggttc cgttcgaaga
     1381 tgccaaagac cagcggcagc agggcggccc tcgccgcctc cggctccccc gcccactgcg
     1441 ttatcaaccc tcgcagcttc tccacgtcat cgtactcgtt tccagtcggg gtaaacgacc
     1501 gaaggtccgc cgcaagtgcg tccgttgtcg ccatgcctcc tcccgttcgt tgatcagcgg
     1561 tcactcgaac cgccgccgcg gagccgctcc cacgccgcct cgatgaggaa catggaggcg
     1621 tcgtcggcga agcggtgttc gggcggcagg ctctcgacca gcgcctcgtt ctcgccccac
     1681 aggccgaact tgttgcggat gcccagcccc cacgtgtggt gaagcaggat caggtcctcc
     1741 ttcgccatgt cccgcagccg ccgccgctcg tcctccgaca ggcccgccac cagccggtca
     1801 accgcctcgg cgaccgtctt cggcatgaaa tccaccggct tgcggtgttt gcggccgagc
     1861 gtcatgacac tcccgctcca tcctccatct tctatcctct atcctctatc ctctccccct
     1921 gcccgctccc cgccccccgc cagcagctgc tccagcttct tgacgcgctc aagcaactcg
     1981 gggagctgcg tgaagaccgt gtacacgcgc cgcgcctgcg ccgcgggcat ggcgggggag
     2041 ccgatcagcg tcacgcccgg ttccacgtcg ttaatcacgc ccgactgcgc cgcgaccgtc
     2101 acgcggtccc ccacctccag gtggccggcg atgccgacct gcccgcccag ggtcacgtac
     2161 tgacccagct tcgtgctgcc ggcgatgccg accaggccca ccagcagccc gtgcgggccg
     2221 atccgcgc
//
LOCUS       DASRIQ010000020         8765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3657780, whole genome shotgun sequence.
ACCESSION   DASRIQ010000020 DASRIQ010000000
VERSION     DASRIQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3657780"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFD06_00495"
     CDS             complement(<1..274)
                     /locus_tag="VFD06_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02042.1"
                     /translation="MSRQPTMPLLAQWLLSRVLPVRLRESILGDLEEERRALGGGTTW
                     LWAQALGIAARYGWERASRVARRPRQSERQSASGARQFGYGASKGDG"
     gene            complement(271..603)
                     /locus_tag="VFD06_00500"
     CDS             complement(271..603)
                     /locus_tag="VFD06_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN02043.1"
                     /translation="MARLGPPGDFEQLVLLAVLRLGDAAYAVPIRREIAARTRRAPAR
                     GAVYITLDRLEGKGYLESRLGDPAAERGGRPRRYYRVKPAGVRALEQSWTALRRMWEG
                     LEPKVRET"
     gene            complement(658..2142)
                     /locus_tag="VFD06_00505"
     CDS             complement(658..2142)
                     /locus_tag="VFD06_00505"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZN02044.1"
                     /translation="MVSVTFPDPVLLRRAAARLVRRPGEFGDLFLEGTAVVSMRREAG
                     RESASETKSERGGAARLLTAEGRSRHVTAEGLDPDGILTLADRVDGGGGTGLPGPGPG
                     AAPGAGREPAADRPEPGIASARAAVRSYLEEIERLLLRAAGGTAWGASWMAQPEQIAL
                     QAQAQIRSQQILIATSEGEIVEDRRDWTWFTVRVEGPGRAAGQAPGPLTGRRVGTIAG
                     GGARNALRLGAVHPPAEVVQRLVTALDAANTTAPAPVGEMPVVLGPGVGGILFHEACG
                     HALEGDRALAGKSALAEKFGEQVAPEFLTLVDDPTLDGLPGSRRFDDEGWRTAPTVLI
                     ERGRVVGLLLDRATARRAGSAPTGSARRETFRDLPLPRMTNTFVREGRWSAGSIVSAV
                     ARGLYVWELGAGQVDTATGEFAFQVRRGAILAGGRMVAASGPCAITGNSLRALAGVRA
                     VGTDLRFDPGAGECGKDGQRARAAVGQPTLLVEGLVVQPTAATT"
     gene            2244..3344
                     /locus_tag="VFD06_00510"
     CDS             2244..3344
                     /locus_tag="VFD06_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZN02045.1"
                     /translation="MRLGGIRVVVDPSWVFVFVLVAISLGGSYLPAVAPHLDPLTTWL
                     LGVLAAVLLFGSVLLHELSHAFLAQRAGIRVPRIRLFLFGGVSEMAAEPHAPSAELRI
                     AAAGPAMSLIIALVSGLASRLFVEPSGMRALCEYLSVSNLLLGLFNLLPGLPLDGGRI
                     LKAWLWSRHGNLLRATRTAGRGGAILGYAFVGLGLLFIWRQDWLSGIWFGILGLFLQR
                     AASASYETALLRDTLSGVKVRSLMTREVVTVPDHISLEELVQEHVLRRPHGAFPVMSG
                     DTLLGMVNIAQVRRVPRDEWVRTPVRQVMTPLLARPAVGPEDDAVTVLERMIREDASP
                     LPVVLDGRLIGLLSRRDVLKVFQVRATLMESR"
     gene            3373..4380
                     /locus_tag="VFD06_00515"
     CDS             3373..4380
                     /locus_tag="VFD06_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZN02046.1"
                     /translation="MRVPLTPARLVASLLLLAAGLVTTLVVCAGIGSSGAGWSDLLRG
                     GDAAVTILRLRLPRLILAAIAGAALGAAGAVFQAVLRNPLADPYILGVSGGAALGAFA
                     VTAAGWQAMAPLWPLRQAAAFAGAILTVLFIFLLSSVGGRVASYPMLLIGVVTNTVYL
                     AIIMFIQTIVELTRLHGLRIWMIGSIPIEGYNVLTPLAVVTAAGVFAIGLFGRDLNLL
                     SAGEDAARSLGVGVERSRILLVGVASLVTAAAVAVTGPIGFVGLIVPHVARILFGPDH
                     RLLAPAAALCGAIFLAAADTVARTALAPTELPVGVITAMAGGPFFLWLYRRQRGRTYF
                     E"
     gene            4377..5204
                     /locus_tag="VFD06_00520"
     CDS             4377..5204
                     /locus_tag="VFD06_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN02047.1"
                     /translation="MTASAVGTGPSAPPLLEARDLVFRHHGAAAPLFQGLSIAIPQGT
                     FAGIIGPNGSGKTTLLRLLAGLVRPGAGGVYLDGHPLDALAPRQRAQTLAVVLQESSL
                     LFNFSVLETALMGRAPHLGLFGLEQAADFASARRALAEVDLRDCENRPVQELSSGERQ
                     RAFLARALAQEPRGILLDEPTAFLDLRHALELYEILRRLNRERGLTVVTVSHDLGLAA
                     RYATRLVLLDRGRIAADGAPDAVVTPALLREVYGTDAEVLRDPATGAPVVLARGAAR"
     gene            5201..6013
                     /gene="xth"
                     /locus_tag="VFD06_00525"
     CDS             5201..6013
                     /gene="xth"
                     /locus_tag="VFD06_00525"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZN02048.1"
                     /translation="MTIASWNVNSVRARRERLLRWLEKNRPDVVCLQETKVTDADFPL
                     AETTALGYYAVLHGQKGYNGVAILAIEQPQAVERGFGDDGDDAHARFIVATVRGVRVA
                     SVYVPNGRAVGSDKWDFKLAWLKRLRAWLDRRAVSEPLVLCGDFNVAPEERDVKNPAF
                     WSGTVLFHPEARRALQEVTSWGLVDVFRRHNQEAGFYSWWDYRMLGFPKNDGLRIDLV
                     LATPPLAERSVAASIDREERKGKLPSDHAPVIAGFNLQSPPSDSGSVHAQSS"
     gene            5997..7097
                     /locus_tag="VFD06_00530"
     CDS             5997..7097
                     /locus_tag="VFD06_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN02049.1"
                     /translation="MLSRRDVLRTGVLVGGGLLAGRSLLSAAPKAKKPIDLLILGGTG
                     FIGPHLVRHAVARGHKVTIFTRGRRNPDLPEGITRLIGDRNGQLQALEGKKWDAVVDD
                     SATNPDYVRQSAGLLKDAVGRYLFTSSTGVYYPYLTRGLDETTAPHLEVSDEKDGSET
                     YGVNKARCEKIVADTFGERGLVVRPGYIVGPGDTSDRFPYWPVRFARGGEILAPGKKD
                     DPVQFIDVRDLTEFMLLLHEAGRSGVYNATGPAQVMTAPEFYKAARKTLNKDATLTFI
                     DDYDFLQAHEIEYSIPWAMLKGNEEGMMSIRIDKAKAAGLRFRPAQTTLKDTLAWWPT
                     VPEERRNKPGFAITPEIEAKALADWKARKVKG"
     gene            7094..>8765
                     /locus_tag="VFD06_00535"
     CDS             7094..>8765
                     /locus_tag="VFD06_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02050.1"
                     /translation="MRRVALLLALAASLASAASASAAEVPPGDYAALRWRLLGPFRAG
                     WALVAAGIPGDATTFYFGAADGGVWKTNDAGVTWKPLFNDQGSASIGALAIAPSNPKV
                     IWVGTGQVHQRWDIAEGDGVYRSTDGGATWKHVGLRETRHVGNIWVDPKNADVAIVAA
                     LGHVFGPNPERGLFRTTDGGRSWTKVLDRGPDVGAVDIASDPAVPGVLYASTWQVRRH
                     PWLDYFQPPAGPGSGIWKSTDNGRTWKQTGTTGLPKTPYGRIDLGVAPGKNARRVYAG
                     LDSEEEPGLYRTDDGGATWTRVNPDPELAGSYMNHIVPDPKNPDIVWASGRGLRRSTD
                     AGKTFAVFKGAPGGDDYHHLYIDPREPRRMMVAADQGAVVTHNGGESWSSWYNQPTGQ
                     FYRLADDNQIPYRVYSGQQDSGTVSIATRSDYGQLTFRDWHPTGGDERDGDVPDPENP
                     DIVYGAGLGGRLSRWDKATGQVQNVSPWPISTYGQRFKPGSPRYSWITPLAISKRSPH
                     TIYQATQVVYRSTDDGRSWKVISPDLSGAVENATGCDGDVPVERASACG"
BASE COUNT         1176 a         3332 c         2979 g         1278 t
ORIGIN      
        1 tgccgtctcc tttgctcgcc ccgtatccga actgccgcgc cccggatgca gactgccgct
       61 cgctttgccg gggccggcgc gcgacgcggc tggcgcgctc ccatccatag cgggccgcga
      121 tgccgagcgc ctgtgcccac agccacgttg tccctccacc cagcgcgcgc cgctcctcct
      181 ccaggtcgcc caggatgctc tcgcgcagcc gtaccggcag gacgcgagac aacagccatt
      241 gagcaagcaa cggcatggtc ggctggcggc tcatgtctcg cgtaccttcg gttccagtcc
      301 ttcccacatg cgccgcagcg ccgtccacga ctgctcgagc gcgcggaccc cggcaggctt
      361 cacccggtag taacgacggg ggcgcccgcc ccgttccgcg gccgggtcgc ccagccggga
      421 ttcgaggtag ccctttccct ccaggcggtc cagcgtgatg tagacggcgc cgcgcgccgg
      481 cgcccggcgc gtgcgcgccg caatctcgcg gcggatggga accgcgtagg ccgcgtcacc
      541 caggcgcagc acagccagaa gcacgagctg ttcgaagtca ccgggcgggc cgaggcgcgc
      601 catatgtaat gcctacaatg taggcaatca cgaggcgcgt caagcaaaaa ttcgcagcta
      661 ggtcgtcgct gcggtgggct gcacgacgag cccctcgacg agcagcgtcg gctggccgac
      721 cgcggcgcgg gcgcgctggc cgtccttgcc gcactccccc gcccccggat cgaaccgcaa
      781 gtcggtgccg acggcgcgca ctccggccag cgcccgcagg ctgttgcctg tgatggcgca
      841 cggcccgctg gccgcgacca tccgaccgcc ggccaggatg gccccgcggc gcacctggaa
      901 ggcgaactcc cccgtcgcgg tgtccacctg ccccgcaccc agctcccaga cgtagagccc
      961 gcgcgccacc gcggagacga tcgacccggc gctccagcgc ccctcccgga cgaaggtgtt
     1021 ggtcatcctc ggcagcggca ggtcgcggaa ggtctcgcgc ctggcactgc cggtcggcgc
     1081 cgaaccggcg cggcgggcgg tggcccgatc gagcagcagc cccaccaccc tcccccgctc
     1141 gatcagaacc gtcggggcgg tccgccaccc ttcgtcgtcg aagcgccgcg agccggggag
     1201 gccgtcgagg gtcgggtcgt ccaccagggt caggaactcc ggcgccacct gctcgccgaa
     1261 cttctcggcg agggccgact tgccggcgag ggcccggtct ccttcgaggg cgtggccgca
     1321 cgcctcgtga aacaggatgc cccccacccc cggcccgagg acgaccggca tctccccaac
     1381 tggcgcggga gcggtggtgt tggcggcatc gagggcggtc accagccgct gcaccacctc
     1441 ggccggggga tggaccgcgc cgaggcgcag ggcgttgcgc gcgcccccgc cggcaatcgt
     1501 gccgacgcgc cggccggtca gcggccccgg cgcctggcct gccgccctcc ccggcccctc
     1561 caccctgacg gtgaaccagg tccagtcgcg gcggtcctcg acgatttccc cctcggaggt
     1621 ggcgatgaga atctgctgcg agcggatctg cgcctgcgcc tggagggcga tctgctcggg
     1681 ctgcgccatc caggacgccc cccaggcggt gccgccggcg gcgcgcagca gcagccgctc
     1741 gatctcctcg aggtaggagc gcaccgccgc gcgcgccgag gcgatccccg gttccggacg
     1801 gtcggcggcc ggctcccggc cggcgcctgg cgccgcccct ggcccgggcc ccggcaggcc
     1861 ggtccctccc ccgccgtcca cccggtcggc cagggtcagg atgccgtccg ggtcgaggcc
     1921 ttcggccgtg acgtggcggc tcctcccctc ggcggtcagc aggcgggccg ccccgccccg
     1981 ctcgctcttc gtctcggagg ccgattccct ccccgcctcg cggcgcatcg agacgaccgc
     2041 ggtcccctcg aggaacagat ctccgaactc ccccggccgc cgcacgaggc gggcggcggc
     2101 gcggcgcagg agcaccggat cgggaaaggt gacggagacc atgtccagga gtctgtccgg
     2161 gtcctcgcgt tcggccgggc accgacattc taccgggcac cctctgtaat acgatgatcc
     2221 ggcaaatggc cccgtatgca ttgatgcgcc tgggcgggat ccgcgtcgtc gtcgatccct
     2281 cgtgggtctt cgtcttcgtg ctcgtggcga tcagcctcgg cgggtcgtac ttgccggcgg
     2341 tggcaccgca cctcgatccg ctcacgacct ggctgctcgg cgtgctcgcg gcggtgctgc
     2401 tcttcggctc ggtgctgctg cacgagctgt cgcacgcgtt cctcgcgcag cgcgccggca
     2461 tccgggtgcc gcgcattcgc ctgttcctct tcggcggcgt ctccgagatg gcggccgagc
     2521 cgcacgcccc gagcgccgag ctgcgcatcg ccgcggctgg cccggcgatg agcctcatca
     2581 tcgcgctcgt ctccgggctc gcgtcgcgcc tcttcgtcga gccgagcggc atgcgggcgc
     2641 tgtgcgagta cctgtcggtc agcaacctcc tgctcgggct gttcaatctc ctccccggcc
     2701 tgccgctcga cggcgggcgc atcctgaagg cgtggctctg gtcgcggcac ggcaacctgc
     2761 tgcgcgccac gcgcaccgcc gggcgcggcg gcgccatcct cggctacgcg ttcgtggggc
     2821 tcgggctgct gttcatctgg cggcaggact ggctctcggg aatctggttc gggatcctcg
     2881 gcctcttcct gcagcgcgcc gcctccgcct cgtacgagac ggcgctcctc cgcgacacgc
     2941 tgtcgggggt gaaggtccgc tccctcatga cacgcgaagt ggtcacggtg ccggaccaca
     3001 tcagcctcga agaactcgtc caggagcacg tgctgcgccg cccgcacggc gccttcccgg
     3061 tgatgagcgg cgacacgctg ctcgggatgg tcaacatcgc gcaggtgcgt cgcgtgccgc
     3121 gcgacgagtg ggtgcgcacc cccgtgcgcc aggtgatgac gccgctcctc gcgcgtcccg
     3181 cggtcggccc cgaggacgac gcggtcaccg tcctggagcg gatgatccgc gaggacgcct
     3241 cgccgctgcc ggtcgtgctg gacggccgcc tcatcggcct cctgagccgg cgcgacgtcc
     3301 tcaaggtgtt ccaggtgcgc gccaccctca tggagtcccg gtgagccgac ggcctctcac
     3361 gttcgccctt ccgtgagagt acctctgacg ccggcgcgtc tcgtcgcgtc gctcctcctc
     3421 ctcgccgcgg gcctggtcac gacgcttgtc gtgtgcgcgg ggatcggatc cagcggcgcc
     3481 ggctggtcgg atctcctgcg cggaggcgac gccgccgtca ccatcctgag gctgcggctg
     3541 ccgcggctga tcctggccgc catcgccggg gcggcgctcg gagccgccgg ggcggtgttc
     3601 caggcggtgc tgcgcaaccc gcttgccgat ccctacatcc tcggtgtctc gggcggcgcg
     3661 gcgctcggcg cgttcgcggt caccgccgcc gggtggcagg ccatggcgcc gctctggccg
     3721 ctgcgccagg cggctgcctt cgccggcgcc atcctgaccg tcctcttcat cttcctgctg
     3781 tcgagcgtcg ggggccgggt cgcctcctac ccgatgctcc tcatcggggt cgtcaccaac
     3841 accgtctacc tcgccatcat catgttcatc cagacgatcg tcgagctgac ccgcctgcac
     3901 ggcctgcgca tctggatgat cggcagcatc ccgatcgagg gttacaacgt attgacgccg
     3961 ttggccgtgg tgacggccgc gggggtcttc gccatcgggc tcttcggacg ggatctcaat
     4021 ctgctgtcgg cgggggaaga tgccgcccgc tcgctcggcg tcggcgtcga gcgctcgcgc
     4081 atccttctcg tcggggtggc ctcgctcgtc accgccgcgg cggtcgcggt gaccgggccg
     4141 atcggcttcg tcggcctcat cgtgccgcac gtcgcccgca tcctcttcgg ccccgaccac
     4201 cgcctgctcg cgccggccgc cgccctgtgc ggcgccatct tcctcgccgc cgccgacacg
     4261 gtggcgcgca ccgccctggc gccgaccgag ctgccggtcg gcgtgatcac cgcgatggcc
     4321 ggcggcccgt tcttcctctg gctctaccgc cgccagcgcg gccggaccta tttcgagtga
     4381 cggcgtcggc cgtgggcacc ggcccgtccg cgccgccgct gctggaggcg cgcgacctcg
     4441 tcttccgcca ccacggcgcc gcggcgccgc tgttccaggg tctgtcgatc gccatcccgc
     4501 aggggacctt cgccgggatc atcgggccga acgggtcggg gaagacgacc ctgctgcgcc
     4561 tgctggccgg gctcgtgcgc ccgggcgccg gcggtgtgta cctcgacggg cacccgctcg
     4621 atgcgctggc gcctcggcag cgggcgcaga cgctcgccgt cgtcctgcag gagtcgtcgc
     4681 tcctcttcaa cttcagcgtc ctcgaaaccg ccctcatggg acgcgcgccg cacctcgggc
     4741 tcttcgggct ggagcaggcg gccgacttcg cgtccgcccg ccgcgccctc gcggaggtcg
     4801 acctgcgcga ctgcgagaac cggccggtgc aggagctgtc gagcggcgag cggcagcggg
     4861 ctttcctggc ccgcgccctg gcgcaggagc cgcgcggcat cctgctcgac gagccgaccg
     4921 ccttcctcga cctcaggcac gccctcgagc tgtacgagat cctgcgccgc ctcaaccggg
     4981 agcgcgggct gacggtcgtc accgtcagcc acgacctcgg cctcgccgcc cgctacgcga
     5041 cccggctcgt cctcctcgac cgcggccgca tcgccgcgga cggcgcgccc gacgccgtcg
     5101 tgacgcccgc gctcctgcgc gaggtgtacg ggaccgacgc cgaagtgctg cgcgatcccg
     5161 ccaccggcgc gccggtcgtc ctcgcccgcg gggcggcgcg ttgacgatcg ccagctggaa
     5221 cgtcaactcc gtgcgggcgc gcagggagcg cctcctccgc tggctggaga agaaccgccc
     5281 cgacgtcgtg tgcctccagg aaaccaaggt caccgacgcc gacttcccgc tcgccgagac
     5341 gaccgcgctc gggtactacg ccgtgctcca cggccagaag ggatacaacg gcgtcgcgat
     5401 cctcgcgatc gagcagccgc aggccgtcga gcgcggcttc ggcgacgacg gcgacgacgc
     5461 gcacgcccgt ttcatcgtgg cgaccgtgcg cggcgtgcgg gtcgcatcgg tctatgtccc
     5521 gaacggccgc gcggtcggct cggacaagtg ggacttcaag ctcgcgtggc tgaagcgcct
     5581 gcgcgcctgg ctcgaccgcc gcgccgtgag cgagccgctc gtcctgtgcg gtgacttcaa
     5641 cgtcgccccc gaggagcgcg acgtgaagaa ccccgccttc tggtccggga ccgtcctgtt
     5701 ccaccccgag gcccgccgcg cgctccagga ggtcacttcg tggggcctcg tggacgtctt
     5761 tcgccggcac aaccaggagg ccgggttcta ctcgtggtgg gactaccgga tgctcggctt
     5821 ccccaagaac gacggcctgc gcatcgatct cgtcctcgcg acgccgccgc tggcggagcg
     5881 ttcggtcgcc gcctccatcg accgcgagga gcgcaagggg aagctgccgt ccgaccacgc
     5941 accggtgatc gccgggttca acctacaatc ccccccttcc gacagcggga gcgtccatgc
     6001 tcagtcgtcg tgatgtcctg agaacgggtg tcctcgtcgg cggcggtctc ctcgccggcc
     6061 gctcgctgct ctccgccgcg ccgaaggcga agaagccgat cgacctgctc atattgggcg
     6121 gcaccggctt catcgggccg cacctggtgc ggcacgcggt ggcgcgcggc cacaaggtga
     6181 cgatcttcac gcgcggccgg cgcaacccgg atctccccga ggggatcacc cggctcatcg
     6241 gcgaccgcaa cggccagctg caggccctcg agggcaagaa atgggacgcg gtggtggacg
     6301 actccgccac caaccccgat tacgtgcggc agagcgccgg cctcctcaag gacgccgtcg
     6361 ggcgctacct cttcacttcg tccaccggcg tgtactaccc gtacctgacg cgcggcctcg
     6421 acgagacgac ggcgccgcac ctcgaggtgt cggacgagaa ggacggcagc gagacctacg
     6481 gcgtgaacaa ggcgcgctgc gagaaaatcg tcgccgacac gttcggagag cgcggcctgg
     6541 tggtgcggcc gggctacatc gtcggccccg gcgacacctc cgaccggttc ccctactggc
     6601 cggtccgttt cgccaggggg ggcgagatcc tggcgccggg aaagaaagac gacccggtgc
     6661 agttcatcga cgtgcgcgac ctcaccgagt tcatgctgct cctgcacgag gcagggcggt
     6721 cgggcgtcta caacgccacc ggcccggcgc aggtcatgac cgcccccgag ttctacaagg
     6781 ccgcccgcaa gactctcaat aaggatgcga ccctgacgtt catcgacgac tatgacttcc
     6841 tgcaggcgca cgagatcgaa tactccatcc cctgggcgat gctcaagggg aacgaagagg
     6901 ggatgatgtc gatccgcatc gacaaggcga aggcggccgg gctccgcttc cggccggcgc
     6961 agaccacgct caaggacacg ctcgcctggt ggccgaccgt tcccgaggag cggcgcaaca
     7021 agccgggctt cgcgatcacg cccgagatcg aggcgaaagc gctggccgac tggaaggcgc
     7081 ggaaggtgaa gggatgaggc gcgttgctct tctcctcgcc cttgcagcat cgctcgcttc
     7141 cgcggcctcc gcatccgccg ccgaggttcc gcccggcgac tacgccgccc tgcgctggcg
     7201 cctcctcggc ccgttccgcg ccggctgggc gctagtcgcg gccgggatcc ccggcgacgc
     7261 caccaccttc tacttcggcg ccgccgacgg cggggtctgg aagacgaatg acgccggggt
     7321 cacgtggaag ccgctcttca acgatcaggg aagcgcctcg atcggcgccc tcgccatcgc
     7381 cccgagcaat cccaaggtca tctgggtcgg gacggggcag gtgcaccagc gctgggacat
     7441 cgccgagggg gacggcgtct accgttccac cgacggcggc gccacctgga agcacgtcgg
     7501 gctgcgcgag acgcgccacg tcgggaacat ctgggtcgat ccgaagaacg ccgacgtcgc
     7561 catcgtcgcg gccctcggcc acgtcttcgg cccgaacccc gagcgcgggc tcttccggac
     7621 caccgacggc gggcgctcgt ggacgaaggt gctcgatcgc ggccccgacg tcggcgccgt
     7681 ggacatcgcg tcggatcccg ccgtccccgg cgtgctgtac gcctcgacgt ggcaggtccg
     7741 ccgccatccc tggctcgact acttccagcc ccccgccggg ccgggcagcg gcatctggaa
     7801 atccaccgac aacggccgca cctggaagca gaccggcacg accggcctgc cgaagacgcc
     7861 gtacggtcgc atcgatctcg gcgtcgcccc gggcaagaac gcccggcggg tctacgccgg
     7921 cctggactcc gaggaggagc cggggctgta ccgcaccgac gacggcggcg cgacctggac
     7981 gcgggtcaac ccggatcccg agctcgccgg cagctacatg aaccacatcg tccccgatcc
     8041 gaagaacccc gacatcgtct gggccagcgg gcgcggcctg cgccgctcca ccgatgccgg
     8101 caagacgttc gccgtgttca agggggcgcc gggtggggac gactaccatc atctttatat
     8161 cgacccgcgc gagccgcgcc gcatgatggt cgcggccgac cagggagcgg tggtgacgca
     8221 caacggcggc gagtcctggt cctcctggta caaccagccg accggccagt tctaccgcct
     8281 cgccgacgac aaccagatcc cgtaccgcgt ctacagcggg cagcaggaca gcgggacggt
     8341 cagcatcgcg acgcgcagcg attacgggca gctgaccttc cgcgactggc accccacggg
     8401 aggggacgag cgggacggcg acgtccccga cccggagaat ccggacatcg tctacggcgc
     8461 cggtctcggc gggcggctgt cgcgctggga caaggcgacg gggcaggtgc agaacgtctc
     8521 gccgtggccg atctccacct acggccagcg cttcaagcct ggaagccctc gctattcctg
     8581 gatcacaccg ctcgccatct cgaagcgctc gccgcacacc atctaccagg ccacccaggt
     8641 ggtgtaccgc tccaccgacg acggccgatc gtggaaggtc atcagccccg atctcagcgg
     8701 cgccgtcgag aacgcgaccg gctgcgacgg ggacgtcccg gtcgagcgcg cctcggcgtg
     8761 cggct
//
LOCUS       DASRIQ010000021         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3392187, whole genome shotgun sequence.
ACCESSION   DASRIQ010000021 DASRIQ010000000
VERSION     DASRIQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3392187"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFD06_00540"
     CDS             <1..295
                     /locus_tag="VFD06_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02051.1"
                     /translation="NPPGPSNPPGQNPPPVMEGGLDSPTTLSPTSPDPTLIVSSEPTC
                     ERQIVTIAQPGDSVLVLEVDGAGRVHGRKAVTLQDGVTAEDAGRFFLAKSCKP"
     gene            complement(303..1019)
                     /locus_tag="VFD06_00545"
     CDS             complement(303..1019)
                     /locus_tag="VFD06_00545"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN02052.1"
                     /translation="MTSYRPPGRIDIGLGGAITPAVKGLIIANVSVYALQIITQRFGG
                     GEVRGIETLFGLNPAFMWEKKWLWQLVTYMFLHSPGWLFHLIINMLMLWMFGTEVERM
                     WGTRRFLKYYFTCGIGAGLVTCATFWTSTTYGASGAVFGVMLAYAMTFPERIIYFWFI
                     FPMRALSFVLLCAGIEVVSLLNLPDGVAHTAHLGGMLFGWLFMKRAWRVQALWAELRW
                     RWRRRRLRVIERDRRNYPFH"
     gene            complement(1016..1606)
                     /locus_tag="VFD06_00550"
     CDS             complement(1016..1606)
                     /locus_tag="VFD06_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZN02053.1"
                     /translation="MLDSNVLVLNRLFQAIQVTTVKKAFCLLYKGHVRAVNPDYTTCD
                     WEDWVDVPPQPQDEFIVTPTVRIRVPRVVLLIDFEGVPRHEVRFTRKNIFYRDRNKCQ
                     YCGHRFATRDLNLDHVVPLSRGGKSTWENVVCCCIPCNSRKGGRTPEEAHMRLTHSPA
                     RPRWHPLVRLSLTSGQYEAWRNFLDAVYWNTELKEG"
     gene            complement(1680..2774)
                     /locus_tag="VFD06_00555"
     CDS             complement(1680..2774)
                     /locus_tag="VFD06_00555"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HZN02054.1"
                     /translation="MSAARRGRAGGRRIRVGVVFGGRSVEHDVSLVSAGAIMDALDRR
                     RYQVLPIPVSREGRWPAGVIERLLGRKAALDVVIPMIHGTDGEDGSLQGLLTLSGVPF
                     VGAGVLGSAVGMDKAVMKSLLQDAGLPTVRHRVVTRAEFDADAGGAAALAERHCGLPL
                     FVKPANGGSSVGVSKVRDRAALEPALRLAFRYDRKVIAERAIDAREIECSVLGNDAPE
                     ASVLGEIVPAGEFYDYVSKYQDKGSRLLIPAPIEPAQAALGRDLAVRAFQALDLSGMA
                     RVDLFLDRATGAFFLNEVNTLPGFTPISMYPKLWEASGLPFPRLVDRLVRLAFERRRQ
                     REGLVTRYRPAPRGEARPAAAAGERKRLRA"
     gene            complement(2771..>3576)
                     /gene="hflX"
                     /locus_tag="VFD06_00560"
     CDS             complement(2771..>3576)
                     /gene="hflX"
                     /locus_tag="VFD06_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZN02055.1"
                     /translation="FARRARSREARTQVELAQLRYLLPRLTRAWTHLERQAGGIGQRG
                     VGETQLETDRRLIRKRVSRLEAELKSIESERRERRKRRGGVPKVALVGYTNAGKSTIL
                     NLLTGAGTFVEDRLFATLDPLVRRADRPGRAPFLVIDTVGFIRKLPHHLVASFRSTLE
                     EAADADLLLHVSDASSPALADQLKATRDTLEGLDLADRPTLMVFNKRDRVPAPVLELL
                     LGDHPGALAVSALAPADGEVLRRAIERGLRASTAERAEAARAAAARQGS"
BASE COUNT          557 a         1292 c         1228 g          499 t
ORIGIN      
        1 caatcccccg ggcccatcca acccccccgg ccagaacccg ccgccggtga tggagggcgg
       61 gctcgactct cccacgaccc tctcgccgac gagccctgat ccgacgctca tcgtctcctc
      121 ggagccgacc tgcgagcgcc agatcgtcac gatcgctcag ccgggcgaca gcgtgctcgt
      181 gctggaggtg gacggggcgg gccgggttca cggccggaag gcggtgaccc tgcaggacgg
      241 agtgacggct gaggacgctg gccggttctt cctggccaag tcctgcaagc cctgatcccc
      301 tctcagtgga aggggtagtt ccggcggtcg cgctcgatca cgcgcagccg ccgacggcgc
      361 cagcgccagc gcagttcggc ccacagcgcc tgcacccgcc aggcccgctt catgaacagc
      421 cagccgaaca gcatcccgcc caggtgggcg gtgtgcgcca cgccgtccgg aaggttgagc
      481 agtgacacca cctcgatgcc tgcgcacagc aggacgaagc tcagggcgcg catcgggaag
      541 atgaaccaga aatagatgat gcgctccggg aaggtcatgg cgtaggccag catcacgccg
      601 aacaccgcgc cgctcgcccc gtaggtcgtg ctggtccaga aggtcgcgca ggtgacgagc
      661 ccggccccga tgccacatgt gaagtagtac ttcaggaacc ggcgggtccc ccacatccgc
      721 tccacctcgg tcccgaacat ccacagcatc agcatgttga tgatcaggtg gaacagccac
      781 ccgggggagt gcaggaacat gtaggtgacc agctgccaga gccacttctt ctcccacatg
      841 aaggcgggat tgaggccgaa gagggtctcg atgccgcgga cttcgccgcc gccgaagcgc
      901 tgcgtgatga tctgcagcgc gtacacggag acattcgcga tgattagccc cttgaccgcc
      961 ggggtgatcg ctccgccgag cccgatgtcg atccggcccg gcggccggta ggaggtcacc
     1021 cttccttcag ctcggtgttc cagtacaccg cgtcgaggaa gttcctccac gcctcatact
     1081 gcccggaagt gagggagagg cggacgagcg ggtgccagcg cgggcgcgcc ggcgagtggg
     1141 tgagccgcat gtgcgcctcc tccggcgtgc gtcccccctt gcgcgagttg cacgggatgc
     1201 agcagcagac gacgttctcc caggtcgact tgccgccgcg cgacagcggg acgacgtgat
     1261 cgaggttcag gtcgcgggtc gcgaaccggt ggccgcagta ctggcacttg ttgcgatcgc
     1321 ggtagaagat gttcttgcgg gtgaagcgga cctcgtggcg cggcacccct tcgaaatcga
     1381 tcagcaggac gacgcgcggc acgcggatgc gcaccgtcgg ggtgacgatg aactcgtcct
     1441 ggggctgcgg cgggacgtcc acccagtcct cccagtcgca ggtggtgtag tcggggttga
     1501 cggcgcggac gtgccccttg tacagcaggc agaaggcctt cttgaccgtg gtcacctgga
     1561 tcgcctggaa caggcggttc aggaccagga cgttgctatc cagcatctcg actccgaccc
     1621 cgcgtccccc gcgctcaccg gccgtgcagg gaaatgatta ggatgcgccc cccggactgt
     1681 caagcccgga gacgcttccg ctcccccgcc gccgcggccg gccgagcctc gcccctcggt
     1741 gcggggcggt atcgcgtcac cagcccctcc cgctggcggc ggcgctcgaa tgccaggcgg
     1801 accagccggt cgacgagccg cgggaacggc agcccgctcg cctcccagag cttcgggtac
     1861 atgctgatcg gcgtgaaccc gggaagcgtg ttgacctcgt tgaggaagaa cgccccggtg
     1921 gcgcgatcga ggaacagatc gacacgcgcc atgccggaaa ggtccagcgc ctggaaggcc
     1981 cgcaccgcga gatcgcggcc gagcgccgcc tgcgccggct cgatcggcgc cgggatgagc
     2041 aggcgggatc ctttgtcctg gtacttggac acatagtcgt agaactcccc cgccgggacg
     2101 atctcgccga gcacggacgc ctccggggcg tcgttcccga ggacgctgca ctcgatctcg
     2161 cgggcgtcga tggcgcgctc cgcgatcacc ttgcggtcgt agcggaaggc gaggcgcagc
     2221 gcgggctcca gcgccgcccg gtcccggacc ttgctgaccc cgacgctgct gccgccgttg
     2281 gccggcttga cgaagagcgg caggccgcag tggcgctcgg cgagcgccgc ggcgccgccc
     2341 gcatcggcgt cgaactccgc gcgcgtgacc acccgatgcc gcaccgtcgg gagcccggcg
     2401 tcctgcagga gcgatttcat gacggccttg tccatgccga ccgccgagcc gaggaccccc
     2461 gctccgacga acggcacgcc ggagagcgtc aggagcccct gcagcgagcc gtcctcgccg
     2521 tcggtgccat ggatcatcgg gatcaccacg tccagcgcgg ccttccggcc gagcagccgc
     2581 tcgatcacgc cggccggcca gcgcccctcg cgcgacaccg gaatcgggag cacctggtag
     2641 cggcgccggt cgagggcgtc catgatggca cccgccgaca cgagcgacac gtcgtgctcc
     2701 accgatcgcc caccgaagac gacaccgacc cggatccgcc tgccgcccgc cctgccgcgc
     2761 cgcgccgcgc tcatgaaccc tgccgcgccg ccgccgcgcg cgccgcctcc gcccgctccg
     2821 ctgtcgaggc acgcagcccc cgctcgatgg cacggcgcag cacctcgccg tccgctggcg
     2881 ccagcgccga caccgccagc gccccggggt gatcgcccag cagcagctcc aggacgggcg
     2941 ccggcacgcg gtcccgcttg ttgaacacca taagggtcgg acgatccgcg aggtcgagcc
     3001 cctcgagcgt atcgcgggtt gccttcagct gatcggcgag ggccggcgag gaggcgtcgg
     3061 agacatgcag cagcagatcg gcgtcggccg cctcctcgag ggtgctccgg aacgacgcga
     3121 cgaggtggtg cggcagtttg cggatgaatc cgaccgtgtc gatgacgagg aacggcgccc
     3181 ttcccgggcg gtcggcgcgc cgcaccagcg gatcgagcgt ggcgaacagg cggtcctcga
     3241 cgaacgttcc cgccccggtc aggaggttga ggatggtgga cttgccggcg ttggtgtacc
     3301 cgacgagcgc cactttcggg accccgccgc gccgcttgcg ccgctcgcgc cgctcgctct
     3361 cgatactctt cagctccgcc tcgagccggg agacccgctt gcggatgagg cgccggtcgg
     3421 tctccagctg cgtctccccc acgccgcgct gcccgatgcc gccggcctgg cgctcgaggt
     3481 gggtccaggc cctggtcagc cgcggcagga ggtagcgcag ctgcgccagc tccacctggg
     3541 tgcgcgcctc acgcgagcgg gcccggcggg cgaaga
//
LOCUS       DASRIQ010000022         9589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1568876, whole genome shotgun sequence.
ACCESSION   DASRIQ010000022 DASRIQ010000000
VERSION     DASRIQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568876"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VFD06_00565"
     CDS             <1..1189
                     /locus_tag="VFD06_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HZN02056.1"
                     /translation="HFGALTTALAATIGTGNIVGVATAVSAGGPGAVLWMWLTGVFGI
                     ATKYSEAVLAVKYRVRGPDGTMAGGPMYVLERGLKMRWLGLLFAGLTAVAAFGIGNMV
                     QANSISTLVKETFEIDTFWTGAVMTAFTAIVILGGIRSIARVCEVLVPFMAIFYVVGC
                     AALLAIGWSTLPETLRLIVTSAFTGQAAIGGFAGAAMREAIRYGIARGLFSNESGLGS
                     APIVAAAAQTRNPVRQALVSSSGTFWDTVVVCAMTGLVAVNTGAWTKGLGGAALTKEA
                     FGLFPVIGPLVLTVGLLTFVFSTILGWAYYGEKAIEYLLGSRAITPYRIAWVAAVMVG
                     SVATLPAVWAFADIANGLMAIPNLISLLALSGVVVNETRIYLWNGDLDRDGQDVPDRR
                     AAA"
     gene            complement(1194..1841)
                     /locus_tag="VFD06_00570"
     CDS             complement(1194..1841)
                     /locus_tag="VFD06_00570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HZN02057.1"
                     /translation="MTCKSLIALVLATAGAVSASRADLPTLSPSEALSRPGPIGRGSD
                     AQAKPLIGTKPPAFPAIRWLDGRTRNLETLSGRVVLIRSFTNACPFCAASLPTLQALH
                     DEYAPRGLVVLGVYHAKPRRPVASEDVVRFARALGANFPVGIDPDWRLLEAWWPGRAE
                     PDWTSVTWLLGRDGVIRYVHPGGEYHASGGRDHDRCRADERDLRATVERLLRETV"
     gene            1889..2428
                     /locus_tag="VFD06_00575"
     CDS             1889..2428
                     /locus_tag="VFD06_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02058.1"
                     /translation="MPHVFEPAATGRAKCRGCGKPIEKGAIRFGERIPNPFAEGEATL
                     WFHPLCGAYKRPESMLEALEQAGSSAPDPEALERAARKSSAQRRLPRIDGADRAPTGQ
                     AKCRHCHEPIAKGSWRIRLVFYEEGRFMPSGFVHLGCREGYFETGDVLDPLLHFSPDL
                     SEDDRAELKRALETPSATA"
     gene            2591..3610
                     /locus_tag="VFD06_00580"
     CDS             2591..3610
                     /locus_tag="VFD06_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HZN02059.1"
                     /translation="MAKMMRAVQVSRAGGPFEIVERPIPEPGAGAVRIRVQACGICHS
                     DALTKDGGWPGQRYPRLPGHEVAGVVDAVGPGTPRLKEGQRVGVGWNGGYCGHCAQCR
                     RGDFFACVTQEITGITRDGGYGDYLVAAHSAVAVLPDGLSPAEAAPLMCAGVTTFNAL
                     RNSGARPGDLVAVLGLGGLGHLAVQYAAKMGFRTVGIARGRDKEPLAKQLGASAYIDS
                     QGQDPAAALQEMGGAQLILATATSGEAMAAVQGGVAVNGTLLILGAAHSMQVNPLLLL
                     GGRRSIRGWASGTSLDSEDTLAFSARTGVRSMNEIFPLERAAEAYDRMMSGKARFRVV
                     LQIES"
     gene            3626..5197
                     /locus_tag="VFD06_00585"
     CDS             3626..5197
                     /locus_tag="VFD06_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN02060.1"
                     /translation="MRSEAPARAGRREWIGLGVLALPCLLYSMDLTVLNLAVPSLSAD
                     LKPGSTELLWIIDIYGFMVAGSLITMGTLGDRIGRRRLLLFGAAAFGVASVLAAFSTS
                     PRMLIAARAVLGLAGATLAPSTLSLIRNMFHDPGQRSIAIAVWISSYSAGAVIGPPLG
                     GILLQSFWWGSVFLVSVPMMVLVLALAPVLLPEFRAPDAGRPDAWSAILSVTAVLLVI
                     YGLKQIAAYGPGWVAALSILAGSAVGFVFVRRQRKLSDPMIDLTLFRTPAFSASLVTY
                     MFATFAVFGPYVFIAQYLQLVLGLSPLQAGLWSTPHAGAFVVGSMLSPLLARRIRPAP
                     LMAAGLVVATAGFLLLMLVGRFGLPVIVASAVIYSLGLSPVFTLANDIILGAAPPERA
                     GAASAISETSSEFGGALGIAALGSLGTAIYRSAMVGIADLDGVPAAAAETARDTLGGA
                     VLVAAQLPDQGTALLATARTAFTDGLQAMALTSALIMVGLAALVMALRRDLGATTDRQ
                     PPPDRAPDAAAVPTA"
     gene            5287..6210
                     /locus_tag="VFD06_00590"
     CDS             5287..6210
                     /locus_tag="VFD06_00590"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HZN02061.1"
                     /translation="MDILGEASPRRRAPLPSFILVLTVAAATAGASAPAVPVMPAARG
                     PAADASLTGQAVVERYLAKQDVPAEMAFIAMVVSSPGAGPKEYRFLTVHHKRADGGRD
                     ALLRVLRPRDVEGVALLVAEPASGAPRMSLFLPEVGRVRPLGEQGLSGAFLGSNFTYE
                     DLRREIPDIHDYERLEDTTVREVPCFRVRAREKSGGVSAYAWRDLLISRDAFDLLRVD
                     FYGKGGKLTKSLNAFEYRSAQVKGETRRPRRAVMRAVGSDAWTDFTVVEGRIGLQLPE
                     DLFTARRQETWTPAEVEEFIFRFGMLVRGGI"
     gene            6237..8939
                     /locus_tag="VFD06_00595"
     CDS             6237..8939
                     /locus_tag="VFD06_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN02062.1"
                     /translation="MWRLPLFFYERRTAVLAAALVAALLAGLAAAGLRLDVSAEAMIP
                     AGSPERKARDEVRSLFGTHDSAAVFAADAALFTRDRLRLLREVHDLLAALPGVARVDS
                     LFSIPDLRDEGGVLVAAPILAAIPDDPGDLARLRARAVAHPLLARSAISADGTAMLLR
                     VVPAGGVDPDAGRALAREIEAVLATRRAGFATLEAVGSAPFQIALEDALRHDLLLLLP
                     ITTSVLYLLLALFLRSPLLAAVPILNGALATVFTLGGMALLGIPVTLLNATLPALILV
                     VGGMEDVHLIIEFRERLREHALDKLAIYETTERIGFAVLLNGLTTMLGFAAVGFGTLP
                     VLRLFGLAATLGMLLRFLVTLAVLPALLGLLAGRIRAAADIPDRGEDRAGRFSAFVVD
                     RLAPRAGLVIACLAAITAAGLLALAGLPRGNDLLGFLGEDDPIARRVRAVETRMAGTE
                     TLHLVLRAAPGEFESPEALERLRRLTDRVRGLDGVDVATSLADIVALLHQELRADGTP
                     GRIPGTSSGVAQVLMFADPAALTPYVTADRGAADVLVRTARHDEAGLLALAQGLEAAV
                     PDQAARPWTVQVAGDGLAAARAAASITTSQALSLGGMTATLFVIVAAIFLSVRCAAAA
                     VAVNLVAVAAMFLLMRLTGIPLNVGTCMVASITLGVVIDDTLHLLVRYNREVRVHRDE
                     REGIRAAARGETLPMLATAAALAGGFAVLGFSRFVPVRQFGLLSAGVIAIAVVADLVV
                     SPVLFARTRIVTLWDLLGLSLRRVLLEDAPIFAGLRPWQARRLVLASDLAVHRAGEAI
                     GGGPGSPPAMYVVLDGAVALAGVPGGPVGPGATFGEEALFDAAAPPVAATAVQETRVL
                     VLSRRSLDSLRRFSPFLASRLQANLGALVAARAARGQAASEIAT"
     gene            complement(8962..>9589)
                     /locus_tag="VFD06_00600"
     CDS             complement(8962..>9589)
                     /locus_tag="VFD06_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02063.1"
                     /translation="AAAFAYAEAAVVVYLRRLVGVVEPWRDASVYDPAIAALEVGREA
                     ATLLMLFALGWACGRNAQTRVGCALVAFGLWDIFYYVWLKVLLGWPASILTPDILFLI
                     PLPWWGPVVAPVLVAIVLAAAGAAAIALDDRGVAVRVGALDLLLTVAGVVLVLYAFMA
                     DAIAVLPATAEMLSQVRPTGFRWGPYAVGLFLLAVGAVRPLRTAARPR"
BASE COUNT         1229 a         3480 c         3423 g         1457 t
ORIGIN      
        1 ccacttcggc gcgctcacga cggcgctcgc cgccaccatc gggaccggga acatcgtcgg
       61 ggtcgccacc gcggtcagcg ccggcggtcc cggcgcggtg ctgtggatgt ggctcaccgg
      121 ggtgttcggg atcgcgacca agtattccga ggcggtcctg gcggtgaagt atcgcgtgcg
      181 cggtccggac gggacgatgg ccggggggcc gatgtacgtc ctggagcgcg gcctgaagat
      241 gcgctggctg gggctcctgt tcgccgggct caccgcggtg gcggcgttcg gcatcggcaa
      301 catggtgcaa gccaattcga tctcgacgct cgtgaaggag acgttcgaga tcgacacctt
      361 ctggaccggc gcggtgatga cggcgttcac cgccatcgtg atcctcggcg gcatccgctc
      421 gatcgccagg gtgtgcgagg tcctggtgcc gttcatggcg atcttctacg tcgtcggatg
      481 cgcggcgctt ctggcgatcg gctggagcac cctgccggag acactgcggc tgatcgtgac
      541 gagcgcgttc accggccagg cggcgatcgg cgggttcgct ggagccgcga tgcgcgaggc
      601 gatccgctat ggcatcgcgc gcgggctgtt ctcgaacgag tcgggcctcg gcagcgcgcc
      661 gatcgtcgcg gccgcggccc agaccaggaa cccggtccgc caggcgctgg tctcctcgag
      721 cgggaccttc tgggacaccg tcgtggtctg cgccatgacc gggctggtcg cggtcaacac
      781 gggcgcctgg acgaaggggc tcggcggcgc ggcgctgacc aaggaggcgt tcggcctctt
      841 ccccgtcatc ggaccgctcg tcctgacggt cggcctcctg acgttcgtct tctcgaccat
      901 cctcgggtgg gcctactatg gcgagaaggc gatcgagtac ctgctcgggt cccgcgccat
      961 caccccctat cgcatcgcct gggtcgcggc ggtgatggtg ggctcggtcg ccacgctgcc
     1021 ggcggtctgg gcgttcgcgg acatcgccaa cggcctcatg gcaatcccga acctgatctc
     1081 gctcctggcg ctgtcgggcg tcgtcgtcaa cgagacgcga atttacctat ggaacggcga
     1141 tctcgatcgc gacggacagg acgtgccgga ccgacgcgcc gccgcctgac cccctagaca
     1201 gtctcgcgta atagccgctc gacggtcgcg cggaggtcgc gctcgtcggc gcggcagcgg
     1261 tcgtgatcgc gcccgcctga ggcgtggtac tcgccgccgg ggtgaacata acggatgaca
     1321 ccgtcccgcc ccaggagcca cgtgacggag gtccagtcgg gctccgcgcg gccaggccac
     1381 caggcttcga gcagccgcca atcgggatcg atgccgacgg ggaagttcgc gccgagcgcg
     1441 cgcgcgaagc ggaccacgtc ctcggacgcg accggccgcc tgggcttggc atggtagacg
     1501 ccgagcacga ccaggccgcg cggggcgtac tcatcgtgca aagcctggag cgtcggaagc
     1561 gacgcggcgc agaaggggca ggcgttggtg aagctccgga tcagcaccac ccggcctgac
     1621 agcgtctcga ggttccgcgt ccggccatcg agccagcgaa tggccggaaa tgccggaggc
     1681 ttcgttccga tgaggggttt cgcctgcgcg tcgctgccgc gtccaatcgg gcccggccgg
     1741 gacagggcct cggacgggga gagtgtcggc agatcggccc ggctcgcaga gacggcgccg
     1801 gctgtcgcca gcacgagggc gatcagggat ttgcaagtca tgagggcctc tattatgagg
     1861 gaggccgaac tgttctacga tgacacccat gccccacgtc ttcgagccgg ccgcgaccgg
     1921 tcgcgccaag tgccgcggct gcggcaagcc gatcgagaaa ggcgccatcc gtttcggcga
     1981 gcgcatcccg aatcccttcg ccgaagggga ggcgacgctc tggttccacc ccctctgcgg
     2041 cgcctacaag cgcccggagt cgatgctcga agcgctggag caggcgggct cgagcgcgcc
     2101 cgaccctgaa gcgctcgagc gcgccgcccg gaagagctcc gcgcagcgac gattgccgcg
     2161 catcgacggc gccgatcgtg cacccaccgg ccaggccaag tgccgccact gccacgagcc
     2221 gatcgccaag ggctcctggc gcatccgtct cgtcttctac gaggagggcc gcttcatgcc
     2281 ctccggcttc gtgcacctcg gctgccgcga aggctacttc gagaccggcg acgtcctgga
     2341 tcccctgctg cacttcagtc ccgacctgag cgaggacgac cgcgccgagc tgaagcgcgc
     2401 actcgaaacc cccagcgcaa ccgcttagtc ggactgcccc cccgtgggtt gcaggtcggg
     2461 gccaccgtct ccgtaaggtg ccccgccccg gtcggacgct ccgccgcgac aggcctgatc
     2521 tggtgggcac gaacgagcgg cttcggtatg ctccgcgaag gcgccggcgg cgccggatcg
     2581 gaggagcgac atggcgaaga tgatgcgggc ggtgcaggtg tcgcgggcgg gcggtccttt
     2641 cgagatcgtc gagcggccga tccccgagcc gggtgcgggc gcggttcgca tccgcgtcca
     2701 ggcgtgcggg atctgccaca gcgatgcgct caccaaggat ggaggatggc ccgggcagcg
     2761 gtatccgcgc cttcccggcc acgaagtggc gggggtcgtc gacgccgtcg gccccggaac
     2821 gccgcgcctc aaggagggcc agcgcgtcgg cgtcggctgg aacggcggct actgcggtca
     2881 ctgcgcgcaa tgccggcgcg gggacttctt cgcctgcgtg acccaggaga tcaccgggat
     2941 cacccgcgac ggggggtatg gcgattacct cgtcgccgcg cacagcgcgg tggccgtgct
     3001 gcctgacggc ctgtcccccg ccgaggcggc gccgctgatg tgcgcgggcg tcaccacctt
     3061 caacgcgctc cggaacagcg gggctcggcc cggggatctc gtcgcggtgc tcggcctcgg
     3121 cggactcggc cacctggcgg tgcagtacgc cgcgaagatg gggttccgca ccgtcggcat
     3181 cgcgcgcggc cgcgacaagg agccgctggc gaaacagctg ggcgcgtcgg cgtacatcga
     3241 cagccagggt caggacccgg cggcggcgct ccaggagatg gggggcgcgc agctgattct
     3301 cgccaccgcc acgtcgggcg aggcgatggc cgcggtgcag ggcggggtgg ccgtcaacgg
     3361 cacgctgctg atcctcggcg cggcgcacag catgcaggtc aacccgctcc tcctgctcgg
     3421 cggccgccgc tccatccgcg gctgggcctc ggggacctcc ctcgacagcg aggacaccct
     3481 ggccttcagc gcccgcacgg gcgtccgatc gatgaacgag attttccccc tcgagcgcgc
     3541 cgccgaggcc tacgaccgga tgatgagcgg caaggcgcgc ttccgcgtcg tgctgcagat
     3601 cgagtcctag ggcgctccgc cgacgatgag atcagaggct cccgcgaggg ccggacgccg
     3661 ggagtggatc gggctgggcg ttctcgcgct cccctgcctg ctctattcga tggatctgac
     3721 ggtgctgaat ctggcggtcc cgtccctgag cgcggacctc aagcccggca gcacggagct
     3781 gctgtggatc atcgacatct acggcttcat ggtcgccggg tcgctgatca cgatgggcac
     3841 gctcggcgac cggatcggcc gccgccggct cctcctgttc ggcgccgccg cgttcggcgt
     3901 ggcgtcggtg ctcgccgcct tctcgaccag tccccggatg ctgatcgccg cccgcgcggt
     3961 gctgggcctc gccggggcga ccctggcgcc ttcgaccttg tccctgattc gcaatatgtt
     4021 ccacgacccc gggcagcgct ccatcgcgat cgcggtctgg atctccagct actcggccgg
     4081 cgcggtgatc ggcccgccgc tcgggggaat cctgctgcag tccttctggt ggggttccgt
     4141 cttcctcgtg agcgtcccga tgatggtgct cgtgctggcc ctcgctccgg tcctgcttcc
     4201 ggagttccgg gcccccgatg cgggccggcc cgacgcctgg agcgcgatcc tgtcggtgac
     4261 ggcggtcctg ttggtcatct acggcctcaa gcagatcgcc gcgtacggtc ccgggtgggt
     4321 ggcggccctg tcgatcctcg ccggatcggc cgtcggtttc gtgttcgtgc gcaggcagcg
     4381 aaaactgtcc gacccgatga tcgatctgac gctgttccgg accccggcat tcagcgcctc
     4441 cctcgtcacc tacatgttcg ccaccttcgc ggtgttcggg ccctacgtct tcatcgccca
     4501 gtatctgcag ctggtgctcg gcctctcgcc gctccaggcg gggctgtggt cgaccccaca
     4561 cgccggcgcg ttcgtcgtcg gctccatgct gtccccgctg ctcgcgcgac ggattcgccc
     4621 cgcaccattg atggcggccg gcctcgtcgt cgcgaccgcc ggcttcctgc tgctgatgct
     4681 ggtcgggcgt ttcggattgc ccgtcatcgt ggccagcgca gtcatctact cgctggggct
     4741 gtcacccgta ttcaccctcg cgaacgacat catcctcggc gccgcgcccc cggagagagc
     4801 gggtgcggcg tcggcgatct cggagacgtc ctcggagttc ggcggagcgc tcgggatcgc
     4861 cgctctcggc agtctcggga ccgccatcta ccgcagcgcg atggtgggga tcgcagacct
     4921 ggacggcgtt ccggcggccg ccgccgagac cgcgcgggac acgctcggcg gcgcggtgtt
     4981 ggtggccgcg caattgccgg accaggggac cgcactcctg gcaacggcca ggacggcctt
     5041 caccgacgga cttcaggcga tggcgctgac cagcgcgctc atcatggtcg gcctcgccgc
     5101 cctcgtgatg gccctccggc gggatctcgg cgcgaccacc gaccggcaac ctcccccgga
     5161 tcgggcaccg gacgcggcgg ccgtgccgac cgcctgagcc accgcgcgtc gccacgagat
     5221 tgtcccagcg gggcaggtgt cgggaaggtg tcgtcgccta ggatattctg ttgtccggag
     5281 gaatccatgg acatcctggg cgaagcgtcg ccgcgccggc gtgccccgct gccgtctttc
     5341 atcctcgtgc tgaccgttgc cgccgcgacc gcgggcgcct ccgctccggc cgtcccggtc
     5401 atgccggccg cccggggccc ggcggcggac gcatcgctga cgggacaggc ggtcgtcgag
     5461 cgctacctcg cgaagcagga cgtcccggcg gagatggcgt tcatcgccat ggtcgtgtcg
     5521 agccctggcg ccggcccgaa ggaataccgg ttcctgaccg tgcaccacaa gcgagcggac
     5581 ggcggacggg acgcgctcct gcgggtgctg cggccgcgcg acgtcgaggg ggtggccctg
     5641 ctcgtggcgg agcccgcctc cggcgcgccg cggatgtccc tcttcctgcc ggaggtcgga
     5701 cgggtccggc cgctcggtga acaggggctt tcgggggcgt tcctcggctc caacttcacc
     5761 tacgaggacc tgcggcgcga gatccccgac atccacgact acgagcggct cgaggacacg
     5821 accgtgcgcg aggtcccgtg cttccgcgtg cgggcccgcg aaaagagcgg cggcgtcagc
     5881 gcctacgcct ggcgcgacct gctgatctcg cgcgacgcct tcgatctgct gcgcgtcgac
     5941 ttctacggga agggcgggaa actgacgaag agcctcaacg cgttcgagta tcgctcggcc
     6001 caggtgaagg gggaaacccg ccggccccgt cgcgcggtga tgcgcgccgt cggctccgac
     6061 gcctggaccg acttcaccgt cgtcgagggg cgcatcggcc tgcaactgcc cgaggacctg
     6121 ttcaccgccc gccgtcagga gacctggaca ccggccgaag tcgaggagtt catcttccgg
     6181 ttcgggatgc tggtgcgcgg agggatctga ggcggccggc gccgggcgca gcggccatgt
     6241 ggcgcctgcc tctgttcttc tacgagcgcc ggacggcggt cctggcggcg gcgctcgtcg
     6301 ccgcgctgct cgccggcctc gccgcggccg gactgcggct cgacgtctcg gccgaggcga
     6361 tgatcccggc cggcagcccg gagcgaaagg cacgggacga ggtgcgatcc ctgttcggga
     6421 cgcacgactc cgccgcggtg ttcgccgccg acgcggccct cttcacccga gaccggctgc
     6481 gcctcctgcg cgaagtccac gacctcctcg ccgccctccc gggcgtggcg cgggtcgaca
     6541 gcctcttctc gatcccggat ctgcgcgacg aggggggcgt cctcgtcgcc gccccgatcc
     6601 tggccgcgat ccccgacgat ccgggggatc tcgctcggct gcgcgcccgg gccgtggcgc
     6661 acccgctcct ggcgcgctcg gcgatctcgg ccgacggcac cgcgatgctc ctgcgcgtcg
     6721 tgccggccgg cggggtcgat ccggacgcgg gccgcgcgct ggcgcgggag atcgaggcgg
     6781 tcctcgcgac ccgccgcgcc gggttcgcca ccctcgaggc ggtcggcagc gcacccttcc
     6841 agatcgcgct cgaggacgcg ctgcggcacg acctgctcct cctcctgccg atcaccacat
     6901 ccgtccttta cctgctgctc gccctcttcc tgcgcagtcc cctcctcgcc gccgtcccga
     6961 tcctcaacgg cgcgctggcg accgtgttca cgctgggcgg catggcgctc ctgggcatcc
     7021 cggtcacctt gctcaacgcg acgctgccgg cgctcatcct ggtggtgggc ggcatggagg
     7081 acgtgcacct catcatcgag ttccgcgagc gcctgcgcga gcacgccctc gacaagctgg
     7141 ccatctatga gaccaccgag cggatcggct tcgccgtgct gctcaacggc ctgacgacga
     7201 tgctcgggtt cgccgcggtc gggttcggca cactgccggt cctgcgcctg ttcggcctcg
     7261 ccgccaccct cggcatgctg ctgcgcttcc tggtcacgct cgcggtgctg ccggcgctcc
     7321 tcggcctgct ggccggacgc atccgggcgg ccgcggacat tcccgaccgg ggggaggacc
     7381 gggccggacg cttcagcgcc ttcgtggtcg accggctcgc cccgcgcgcc gggctggtca
     7441 tcgcctgtct ggccgcgatc acggccgccg gcctgctggc tctggcgggg ctgccgcgcg
     7501 gcaacgatct cctcggattc ctcggcgagg acgatccgat cgcccggcgc gtgcgcgccg
     7561 tcgagacgcg catggccggg accgaaacgc tgcacctggt cctgcgcgcc gcgccggggg
     7621 agttcgaatc tcccgaagcg ctggagcgac tgcggcgcct caccgaccgc gtgcgtggcc
     7681 tcgacggggt ggacgtggcg acgtcgctcg ccgacatcgt cgcgctgctg caccaggagc
     7741 tgcgcgccga cggcacgccg ggacgcatcc ccggcacctc ctccggagtc gcccaggtcc
     7801 tgatgttcgc cgatccggcg gcgctcacgc cctatgtcac cgccgaccgc ggcgcggcgg
     7861 acgtcctggt gcgcacggcg cgccacgacg aggcggggct gctggcgctg gcgcaggggc
     7921 tcgaggccgc cgtcccggac caggccgccc gtccgtggac cgttcaggtc gccggcgacg
     7981 gcctggccgc cgcccgggcc gccgcgtcga tcaccacgag ccaggcgctc tcgctcggcg
     8041 gcatgaccgc gacgctgttc gtgatcgtcg ccgcgatctt cctgtcggtg cgctgcgccg
     8101 cggcggcggt ggcggtcaat ctggtcgccg tcgcggcgat gttcctgctg atgcggctga
     8161 ccggcatccc gctcaacgtc ggcacctgca tggtcgcctc gatcaccctc ggggtcgtga
     8221 tcgacgacac gctgcacctc ctggtgcggt acaaccgcga ggtccgcgtg caccgggacg
     8281 agcgcgaggg gatccgcgcc gcggcgcgcg gggaaacgct gccgatgctg gcgacggccg
     8341 ccgcgctcgc gggcggcttc gccgtcctcg gcttctcgcg cttcgtgccg gtgcggcagt
     8401 tcggcctcct gagcgccggg gtcatcgcca tcgcggtcgt cgccgatctc gtcgtctcgc
     8461 cggtgttgtt cgcgcggacg cgcatcgtga cgctgtggga cctgctcggg ctctccctgc
     8521 gccgcgtgct tctggaggat gcgccgatct tcgcggggct gcggccgtgg caggcccggc
     8581 gcctggtgct cgcctccgac ctggccgtgc accgcgccgg cgaggcgatc ggcggcggcc
     8641 cgggctcccc cccggcgatg tacgtggtgc tcgacggcgc ggtcgcgctg gccggcgtgc
     8701 ccggcgggcc ggtcgggccc ggcgcgacgt tcggcgagga ggcgctgttc gacgccgccg
     8761 cgccgccggt cgccgccacg gcggtgcagg agacgcgcgt cctcgtcctc agccggcgct
     8821 ccctcgactc gctgcgccgg ttctcgccgt tcctggcgtc gcggctccag gccaatctcg
     8881 gcgctctcgt ggccgcgcgc gcggcacggg gccaggcggc atcggagatc gcgacctaag
     8941 gcgcccgacc tcgagacgcc gtcacctcgg ccgcgccgcg gtgcgcaggg gccggaccgc
     9001 tccgacggcc agcagaaaca gaccgacggc gtacggcccc cagcggaatc ccgtggggcg
     9061 gacctgggag agcatctccg cggtcgcagg caggacggcg atcgcgtccg ccatgaaggc
     9121 gtagaggacg aggaccaccc cggcgaccgt cagcaacaga tcgagtgcgc cgactcgaac
     9181 cgcgacgccg cgatcgtcca gcgcgatcgc cgccgctccc gcggcggcca ggacgatcgc
     9241 gacgaggacc ggcgccacga ccggtcccca ccacggaagt ggaatcagga agaggatgtc
     9301 gggggtcagg atcgacgccg gccagccgag gagcaccttc agccagacgt aatagaagat
     9361 gtcccagaga ccgaacgcga ccagggcgca cccgacccgg gtctgcgcgt tgcggccgca
     9421 ggcccagccc agcgcgaaga gcatcaagag cgtcgccgcc tcgcgaccca cctcgagggc
     9481 ggcgatggcc gggtcgtaga ccgacgcgtc gcgccacggc tcgaccacac ccaccaggcg
     9541 gcggagatag accacgaccg ccgcttccgc ataggcgaaa gcggccgcg
//
LOCUS       DASRIQ010000023         5678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1829652, whole genome shotgun sequence.
ACCESSION   DASRIQ010000023 DASRIQ010000000
VERSION     DASRIQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829652"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3006)
                     /locus_tag="VFD06_00605"
     CDS             complement(<1..3006)
                     /locus_tag="VFD06_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02064.1"
                     /translation="MHRRRRRTPFLSVVRAKVALGALLAAVSPLPAAPAESIPPPLQP
                     WIPWVLHGHEEAFCPVVAGGTQGRAAGTEGESGPVVVRACLWPSRLDLDLGDRGGQFR
                     QDWQVYRDAWVALPGDARAWPQDVRVDGRPAPLTARSEAPAVRLTKGRHRVEGRFTWP
                     SLPPLLPVPADSALLGLTVRGRAVPFPVRDAEGRVWLEKLASTEEEESRLDIVVHRLL
                     DDDVPPVLITRVELRVSGRSREVDLGVALPPGFIPMSLAGPLPARLAPGGRLLAQVRA
                     GTWTLELRARHDGPAGDLTPPSREGIGTAEEIWVVRTHPAIRVVKIEGAQALDPQQTT
                     LPEEWRGFPAYLMRAGDALRIVEVQRGDADPAPDRLTLQRTLWLDFDGGGFTANDRIA
                     GQFHRSWRLEMPAPGVLGRVTIDGVDQLITRGKEEKGAAGVEIRQGEANIGADSRLPR
                     GGALPATGWDAEFQAAGIDLRLPPGWRILHASGADESAATWVASWNLLDFFLVLLIAM
                     GVARLFGWGTLWLALGGLGLSWIEPGAPRWTWAAAVATTALARALPAGRMKRAARLLQ
                     GAAIVALLPILIPFLAVQARSAMYPALEPPMNLWPVVGPGGIGETRLEEYGSAAGGFI
                     EGNAASEMFSLGYLESRAQSPKPAAKAEEKLRADLSNLAPDPKAAAQTGPGLPDWQWR
                     RSWLRWSGPVEPGQTIRLWLLPPWLNFLLAWCRVGLLTALLLLLVLGAAREWGTTLRL
                     GPLDRFLKAFATAILAACLLTGPEARAADVPSPEALEELRARLLEAPDCLPDCVAFPR
                     LALEADPGRLRLRLELHAATLAAVPLPGGVDQWLPGHALLDGAPAPLRRDTEGRLWIA
                     LAPGTHQVLLEGALPDRDAVPISLPLAPRDVSARASGWRVEGLQEGRLAEGTLQLSRL
                     RAASARGTESAETLAPFVRVTRRLELGLRWQATTEVSRLTPAGAAVLIEVPLLPGESV
                     TSGEQPVIGDRAIVRLGPQSAGTRWTSVLL"
     gene            complement(3080..4795)
                     /locus_tag="VFD06_00610"
     CDS             complement(3080..4795)
                     /locus_tag="VFD06_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN02065.1"
                     /translation="MIRGLSRRVLLPALLALLPATAWGQTQTIIHDCDSGPVSTLGGW
                     QMPGVTPISGDSTCGPGGIIFLWASYEAYYQWLRQTPIMGDVTIEAQVMLLDRDGGSD
                     DAFSLLLHWSGSTDPTVCCEPVHADSGLRLLFSLARNRAEVYEETNGVIGPLLQTLPF
                     SLPIDTMRSIKVGYRGNILDLTVDSALVGTVTVPATPPSLFGFDARGISLRLEPILLT
                     FGCVNDVDCDGITDGADNCPTTPGADQTDTDHDGQGNLCDADDDNDGIADATDVCPLV
                     PDPQQADFDQDHVGDACDVCPSDPTNDPDGDHICGQSDNCPNDSNAGQENRDTDTQGD
                     ACDLNDGVIQVSWPTQTRMTWQRETGLGSFNVYKRNLADLRATGVYVLDPASFPGPLD
                     ERICNIPGDMTQSEPLNFGEGIAYFVSGTANGVDMGLGADSAGQVRANDYPCGVCRAF
                     QRVMHRPDGPTTPQNRLIVSQAEWCSFRPSACSSPPVNFNTHAALVVAMGSVPNTCFD
                     THITCIRDGPPTSYEVIVEYDSELPGPTCECFQVFIEPFDVVAIPHPVTRATFEGTDR
                     IIPCP"
     gene            complement(4927..5448)
                     /locus_tag="VFD06_00615"
     CDS             complement(4927..5448)
                     /locus_tag="VFD06_00615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN02066.1"
                     /translation="MRILPAQGPEEIDAARAMFREYAAGLGFDLSFQDFEAELALLPG
                     EYAPPRGALLLARTDGDAAAGADAPSPAAAPGADFAGCVALRPLREPGVCEMKRLFVR
                     PAWRGSGLGRRLAEAILAEGHRLGYTRMRLDTVPAMRSAIGLYESLGFHDIPPYRHNP
                     VPGTRYLEARLDP"
     gene            complement(5450..>5678)
                     /locus_tag="VFD06_00620"
     CDS             complement(5450..>5678)
                     /locus_tag="VFD06_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZN02067.1"
                     /translation="PAAVTLVKSLTIPTYAPSLGGVETLIVRPSLTSHAGMKREERLA
                     IGITDDLLRISCGIEAAEDLVDDFKQALDKV"
BASE COUNT          839 a         1996 c         2076 g          767 t
ORIGIN      
        1 caggaggacg gaggtccatc gcgtgccggc cgactggggg ccgagccgca cgatggcgcg
       61 atcgccgatc accggctgct ccccggaggt caccgattcc cccggcagca gcggcacttc
      121 gatcagcacg gccgcgccgg cgggtgtgag gcggctgacc tcggtcgtgg cctgccatcg
      181 caggccgagc tcgaggcgcc gcgtcacccg cacgaacggc gcgagcgtct ccgccgattc
      241 ggttccgcgc gcgctcgcgg cgcgcagccg cgacagctgc agcgtcccct cggcgagccg
      301 cccttcctgg agcccctcga cgcgccatcc cgaagcgcgg gcggagacgt cgcgcggcgc
      361 gagcggcagg gagatcggca cggcgtcgcg atccgggagg gccccctcga gcaggacctg
      421 gtgcgttccc ggagcgagcg cgatccagag ccgaccctcc gtgtcccggc gcaggggagc
      481 gggcgcgccg tcgagcagcg cgtgtcccgg cagccactgg tccacgcctc ccgggagcgg
      541 cacggcggcg agcgtcgcgg cgtgcagctc cagccggaga cggagacgcc cgggatcggc
      601 ctcgagggcg agacgcggga acgccacgca gtcggggaga cagtcggggg cttcgagcag
      661 ccgcgcgcgc agctcttcga gcgcctcggg cgacggcaca tcggcggcgc gggcttccgg
      721 gccggtgaga aggcacgccg cgagaatcgc ggtcgcgaac gccttgagga atcgatccag
      781 cggtccaagg cggagcgtcg tcccccattc ccgcgccgcc cccaggacga gcagcaacag
      841 gagcgccgtg agcagcccga cgcggcacca ggcgaggagg aaattgagcc aggggggcag
      901 gagccagagg cggatcgtct gccccggttc gaccgggccg ctccagcgca gccaggagcg
      961 ccgccactgc cagtccggga gtcccggccc cgtctgcgcc gcggccttcg gatcgggggc
     1021 gagattcgag agatcggccc tcagtttctc ctccgccttc gccgccggct tgggcgactg
     1081 ggcgcgcgac tcgaggtagc ccagcgagaa catctcgctc gcagcgtttc cctcgatgaa
     1141 gccgccggcg gcggagccgt actcctccag cctcgtctcg ccgatcccgc cggggccgac
     1201 caccggccag agattcatcg gcggctcgag cgccggatac atcgcggagc gggcctggac
     1261 tgcgaggaat gggatgagga tcggcagaag cgccacgatc gcggcgccct ggaggagccg
     1321 cgccgcgcgc ttcatgcggc cggccgggag cgcgcgcgcc agggcggtcg tcgcgaccgc
     1381 cgccgcccag gtccagcgcg gggcgcccgg ctcgatccag gacagcccga gtccgccgag
     1441 cgccagccac agtgtgcccc agccgaagag ccgcgcgacc cccatggcga tcagcaggac
     1501 caggaagaag tcgagcaggt tccaggacgc gacccacgtc gcggcggact cgtccgcgcc
     1561 ggaggcgtgc aggatgcgcc acccgggcgg caggcggaga tcgatcccgg ccgcctggaa
     1621 ctcggcgtcc cagccggtcg ccggcagcgc gccgccccgc ggcaggcgac tgtcggcccc
     1681 gatgttcgcc tccccctggc ggatttcgac tccggccgca cccttttctt ccttgccccg
     1741 cgtgatgagc tgatcgacgc cgtcgatcgt gacgcgcccg aggacgcccg gagccggcat
     1801 ctccaggcgc cacgaccggt ggaactgacc ggcgatccgg tcgttcgccg tgaagccgcc
     1861 tccgtcgaag tcgagccaca gcgtgcgctg cagcgtcagc cgatccggcg ccggatcggc
     1921 gtcgccgcgc tgcacctcga cgatccgcag cgcgtccccg gcgcgcatca ggtaggcggg
     1981 gaagccgcgc cactcctcgg ggagggtggt ctgctgcggg tcgagcgcct gcgccccttc
     2041 gatcttcacg acccggatcg ccgggtgcgt ccgcacgacc cagatctcct cggccgttcc
     2101 gatcccctcc cgcgacggag gcgtgagatc cccggcggga ccatcgtgcc gggcgcgcag
     2161 ctcaagggtc catgtccccg cgcggacctg cgccagcagg cggccgccgg gtgcgagccg
     2221 ggccgggagc ggaccggcga gggacatggg gatgaagccg ggggggagcg cgacgcccag
     2281 gtcgacctcg cggctccgcc cggagacgcg cagctcgacg cgcgtgatca ggaccggcgg
     2341 gacgtcatcg tcgagcaggc ggtgcaccac gatgtcgagg cggctttcct cctcttcggt
     2401 gctggcgagc ttctccagcc agacgcgtcc ctcggcgtcc cgcaccggaa acggcaccgc
     2461 gcgcccccgg acggtgagcc cgaggagcgc cgaatcggcc gggaccggga ggagcggtgg
     2521 cagcgacggc caggtgaacc gcccctcgac gcggtgccgc cctttcgtga ggcgcaccgc
     2581 cggcgcctcg gagcgcgccg tcagcggcgc cgggcgcccg tcgacccgga cgtcctgcgg
     2641 ccacgcccgc gcgtcgccag gcagcgcgac ccaggcgtcg cggtacacct gccagtcctg
     2701 gcggaactgt ccgccgcgat ccccgagatc cagatcgagg cgcgaaggcc acaggcaggc
     2761 gcggacgacc accgggcccg attccccttc ggtcccggcg gcgcgacctt gcgtcccgcc
     2821 ggcgacgacg ggacagaacg cctcttcgtg gccgtgcagc acccacggga tccagggctg
     2881 gagcggcggc gggatcgatt cggccggcgc ggcgggaagg ggcgacaccg cggccagaag
     2941 cgcgccgagc gcgaccttcg cgcgaaccac ggacaggaac ggggtgcggc ggcgcctgcg
     3001 gtgcatcgaa gcccccctcg aggaaacgcg gcgcggatct tagatcaggc cgcgtccccg
     3061 ccgcgctgtc gggtctgcct caggggcagg ggatgatgcg atcggtgcct tcgaaggtcg
     3121 ccctcgtcac cggatgcggg atcgcgacga cgtcgaacgg ctcgatgaac acctggaaac
     3181 actcgcacgt cgggcccggc agctccgagt cgtattcgac gatcacctcg tacgaggtcg
     3241 ggggaccgtc gcggatgcac gtgatgtgcg tgtcgaagca ggtgttcggc acgctgccca
     3301 tcgccaccac cagcgccgcg tgcgtgttga agttgacggg cggagaggag cacgcggaag
     3361 gacggaagga gcaccactcg gcctggctga cgatcagcct gttctggggc gtcgtcggac
     3421 catcgggccg gtgcatcacc cgctggaacg cacggcagac gccgcaggga tagtcgttcg
     3481 cgcgcacctg gccggcgctg tccgccccga ggcccatgtc cactccgttc gccgtgccgc
     3541 tcacgaagta ggcgatcccc tctccgaagt tcagcggctc cgactgggtc atgtctcccg
     3601 ggatgttgca gatccgctcg tcgagaggtc cggggaagct cgccgggtcg aggacgtaga
     3661 cgccggttgc ccgcagatcc gcgaggttcc gcttgtagac attgaaggag ccgagcccgg
     3721 tctcccgctg ccaggtcatg cgggtctggg tgggccagga cacctggatg acgccgtcgt
     3781 tgagatcgca ggcgtctcct tgagtgtccg tgtccctgtt ctcctggccc gcgttcgagt
     3841 cgttcgggca gttgtcgctc tgaccgcaaa tgtggtcgcc gtcggggtcg ttggtcggat
     3901 cggacggaca gacgtcgcag gcgtcgccga cgtggtcctg gtcgaagtcc gcctgctgtg
     3961 gatccggtac gagcgggcag acgtccgtcg cgtcggcgat cccgtcgttg tcgtcgtcgg
     4021 catcacagag gttcccctgc ccgtcgtgat cggtgtcggt ctgatcggcg cccggcgtcg
     4081 tcgggcagtt gtcggccccg tcggtgatgc cgtcgcagtc gacgtcgttg acgcagccga
     4141 acgtcaggag aatcggctcg aggcgcaggg agatgccgcg cgcgtcgaat ccgaacagcg
     4201 acggcggggt ggccggcacc gtgacggttc caacgagggc actatccacg gtgaggtcca
     4261 ggatgttgcc acggtagccg accttgatcg atcgcatggt gtcgatcggc agcgagaagg
     4321 gcagcgtctg cagcagcggg ccgatgacgc cgttcgtctc ctcgtacacc tcggcgcggt
     4381 tccgcgcgag ggagaacagg aggcgcagcc cggagtcggc gtgcaccggc tcgcagcaca
     4441 ccgtcgggtc ggtggacccg ctccagtgca ggagcaggct gaaggcatcg tcggacccgc
     4501 cgtcgcgatc gagcagcatc acctgggctt cgatcgtgac gtcgcccatg atcggggtct
     4561 gccgcagcca ctggtaatag gcttcgtagg aggcccacag gaagatgatg ccgcccggcc
     4621 cgcaggtgct gtcgccgctg atcggggtga cgccgggcat ctgccagccg ccgagagtgg
     4681 acaccgggcc gctgtcgcag tcgtggatga tcgtctgggt ctgcccccag gcggtcgccg
     4741 gcaggagggc gagaagcgcc gggaggagaa cccgtcgaga cagtcctcgg atcacggatt
     4801 cctccttgag agcccggttg tcctgcggcg aaagagaaac cagagccagt gtctgcatgt
     4861 tctgtaccct caagacccca agctgtcaac tgttggtcgc ggaagaaggg cggaggtttc
     4921 gcagaatcag ggatcgagcc tggcctcgag gtaccgcgtg cccgggaccg ggttgtgccg
     4981 gtaggggggg atgtcgtgga agccaagtga ttcgtagagc ccgatggcgg atcgcatcgc
     5041 cgggacggtg tcgagccgca tgcgcgtgta gccgagacgg tgcccttccg cgaggatcgc
     5101 ctcggccagc cgtcgtccga ggccgctgcc gcgccacgcc ggccggacga acaggcgctt
     5161 catctcgcac acccccggct cccgaagcgg ccggagcgcc acgcagccgg cgaagtccgc
     5221 ccccggcgcg gcggcgggcg acggggcgtc cgcgccggcc gcggcgtcgc cgtccgtgcg
     5281 cgccaggagg agggcgccgc gcggcggcgc gtactccccc gggaggaggg cgagctcggc
     5341 ttcgaaatcc tggaacgaca ggtcgaagcc gagcccggcc gcgtactcac ggaacatcgc
     5401 acgggccgcg tcgatctcct ccggcccctg cgccggcagg atgcgcaccc tacaccttgt
     5461 cgagcgcctg cttgaagtcg tccaccagat cctcggccgc ttcgatgccg caggagatgc
     5521 gcagcagatc gtcggtgatc ccgatcgcca gccgctcctc ccgcttcatc ccggcgtgcg
     5581 acgtcaggga cggtcgcacg atgagcgtct cgacgccgcc gaggctgggg gcgtacgtcg
     5641 ggatcgtcag gctcttcaca agcgttaccg ccgccggc
//
LOCUS       DASRIQ010000024         8703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1829663, whole genome shotgun sequence.
ACCESSION   DASRIQ010000024 DASRIQ010000000
VERSION     DASRIQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829663"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFD06_00625"
     CDS             complement(<1..596)
                     /locus_tag="VFD06_00625"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02068.1"
                     /translation="MTGYLLRRGLLALPLLLGAASIVFLLLHLVPGDPVQAMLGPAAR
                     EGDIADLRARLGLDRPLAEQYLRFLGGIGRGDLGLSLHYGDPVRRLLAERLPATALLA
                     GATILVALLLSLPAGIAAGLRPGSGLDRALGASATAALALPSFWLGPVLLLLFAIRLR
                     WLPVSGADVPEAVLLPAVTLALPMAALLSRLLAGAIRQ"
     gene            complement(593..1108)
                     /locus_tag="VFD06_00630"
     CDS             complement(593..1108)
                     /locus_tag="VFD06_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN02069.1"
                     /translation="MRSRRLRFLAAAATLAALPWSPAVPSEPQHDPVLGRVRPAAILD
                     IAPEWRRGHDDYHPDPSDIDVIRSAPDGSLVMVYFGSWCSDSVIAVPRFLKIIGAARD
                     GHLKARYVGVDRTKKEPAGRLEGVGLQLVPTFVLSVRGREIGRIVETPVTTLEHDLAL
                     LVRKALTSPEP"
     gene            1328..2059
                     /locus_tag="VFD06_00635"
     CDS             1328..2059
                     /locus_tag="VFD06_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HZN02070.1"
                     /translation="MLAGALLLPIATPPLPAAAAADDPDAGAPAASVSDPMLARILEQ
                     WDKRQQETQTLVASFTERKELNLLAKPVISKGEFFYSRPNRVRWEYKEPEHKVFVITE
                     DLYTAYYPALKRAEEVPIKKFVGKRLFRFLGVGQKIGDLARYYDFSLAPTSDVKGTHL
                     LRLTPRKRTVRDRVSEMKIWVDDTTFLPRQLQYVESDGDSTLLTFEGMQSNVEVAANR
                     FKVDLPGDVAVSQTFNGFALGQQSF"
     gene            complement(2274..2834)
                     /locus_tag="VFD06_00640"
     CDS             complement(2274..2834)
                     /locus_tag="VFD06_00640"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HZN02071.1"
                     /translation="MTASCAIRTVLLAGCLALLLSCGGDSDGSGTGIIQVNLTDAPID
                     LTTVEKVEVTITDVIVYPGDDDDMPSVNGGPIVLLTHPETFNLLTLTDGATTLLASGE
                     VLAGDYNRIRLEVTEATLTYKDSTEAVPLKIESGKVDIPIRFHVGEDETTALTLDFDA
                     GASVQVNETSSNTLILRPVVTPLPHP"
     gene            complement(2995..4626)
                     /gene="groL"
                     /locus_tag="VFD06_00645"
     CDS             complement(2995..4626)
                     /gene="groL"
                     /locus_tag="VFD06_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464512.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZN02072.1"
                     /translation="MAAKEIIYSEECRQAILRGVNKLADAVKVTLGPRGRNVVLEKKF
                     GSPTSTKDGVTVAKEIELEDPRENMGAQLVREVASKTSDVAGDGTTTATVLAQAIYRE
                     GVKAVTAGANPMDLKKGIDRAVEKAVEEIKKLSRTVGGKSIAQVGTISANNDETIGEI
                     IAQAMEKVGKDGVITVEEARGMETSLEIVEGMQFDRGYLSPYFVTDAERMECILENAY
                     ILIHEKKISNMRELLPLLEQVAKGGRPLLIIAEDVEGEALATLVVNKLRGTLQVCAVK
                     APGFGDRRKAMLEDIAILTGGKCITEDLGIKLENIKVEDLGQAKKLTADKDNTTLVEG
                     KGKRSDVEGRVKQIRAQIEETTSDYDREKLQVRLAKLVGGVAIIKVGAATETEMKEKK
                     ARVEDAMHATKAAVEEGIVPGGGVALLRAVPAIEKLKEKSEDIQSGINIVRRALEEPL
                     RQISGNAGFEGSIIVNQAKQEEKASRGFDAYSGKWVDMFEAGIIDPTKVTRTALQNAA
                     SIAGLMLTTEALVHELPEKEKAAQAGPRGMGGDMY"
     gene            complement(4643..4930)
                     /gene="groES"
                     /locus_tag="VFD06_00650"
     CDS             complement(4643..4930)
                     /gene="groES"
                     /locus_tag="VFD06_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZN02073.1"
                     /translation="MKVKPLNDRVLVRRMEAEEKVKGGIIIPDTAKEKPLEGKVVSVG
                     SGRLDDNGKRIPMEVKVGDRILVGKYSGTEVKIDDIEHVILREDEILGIVS"
     gene            5114..6994
                     /locus_tag="VFD06_00655"
     CDS             5114..6994
                     /locus_tag="VFD06_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN02074.1"
                     /translation="MMVPGSAFEDLTPEAIEARLRALADRLVGSSAFDELDLRHLLDL
                     LTQRTPLLRQEYDPGTLDRVLAGDPRPAAILLPAALAPEHPRDEAALRAWSVPESLRA
                     CGDKCLYDVGVAGRMTYRGLPLQEIGPRSYDLAGQVLHLLADDRTLREFFERNRIRNL
                     PLDEEVLFLRQCAGRFDLYARILQAWRESMGQAAPAPAEREGSPIASGSAILPVPPPS
                     RPPQRRSAPRPKRRETQPPEPPVDDPDGEEDGFKDADRDARLAWFERTLLLRTLDLER
                     LRRELRDDVVDQDEAVHRVCDDLSVFAAGMRERQRPLSYFLVGPTGVGKNHLMESLAR
                     RLEATWECEVPTLILEGPQYAYPSDVNELKGATRGFIRSDEEGLLAEFHERARRAPLS
                     VLLVDEVEKAHPQLMRFFLALMDRGSTMDNRGRLLRFPATILAFTSNAGYSELEVRRG
                     PIGYAAEEAAGSSRRRDAARALKRGLAPEFLARVQVLHFRPLGRASAARILDREIGRL
                     ARRFLRRHGLRLEVLPAARAALVEQGFSVEEGARHLAGRIDRVCNVEVQLRLRPAGVP
                     LTSERRRLLTLIREARRGERAVDVEALTDAVRVELRPERTGRRIVIDYRRDRFVYREE
                     EE"
     gene            6991..7662
                     /locus_tag="VFD06_00660"
     CDS             6991..7662
                     /locus_tag="VFD06_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02075.1"
                     /translation="MRPQPPLVPVLQSIIERTYGLEPLYGGVEPFLIGDAGLRAFHRR
                     AGVGGDLEPGRPRLILRSERGGWRAVVYYPDALVRHLERHDPRRGLDDRNIDAFAMLV
                     EELDHLLVVASRLAAGRTFRLVELEMHALVTKYLVVLHLLGRLTGRRRVSEFHRVWVR
                     HHLFEKYARAPDGEAQRYLDAARLAWRYVAWLDRLAAAARHTELRRFHARPFEEQARR
                     IALNG"
     gene            complement(7674..>8703)
                     /locus_tag="VFD06_00665"
     CDS             complement(7674..>8703)
                     /locus_tag="VFD06_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZN02076.1"
                     /translation="RDDIPTYSIRYAFMARPGVGYIKIENFTRTTEREMEEKIQALRR
                     EGMTQLVLDLRRNPGGLLEQAVRVSEQFLRKGQMVVYTQGRIRGSDQEFRASGTGRHP
                     DLPIVCLVDKFSASASEIVSGALQDHDRALIVGKPTWGKGLVQTVYPLSQGTALALTT
                     ARYYTPSGRLIQRDYNSLEDYLSYEDSEATEPPHGEVRQTDAGRTVYGGGGIRPDVLV
                     DREPTSKFVDTLQRNQAFFNFAVDYNTHHKGLAKGLDVTPEMVDEFLKFLDRRKVTWT
                     KSEVDVDRDRLRAEIRQAIASTLWGMEEGYRVAMEIDKQVQRAIELFPEAQQLASLPR
                     APRPTQPQ"
BASE COUNT         1267 a         2992 c         3014 g         1430 t
ORIGIN      
        1 tcctgtcgga tcgcgccggc gagcagccgc gacaggagcg ccgccatcgg cagggcgagg
       61 gtgacggcag ggaggagaac cgcctccggc acgtccgccc ccgacacggg gagccatctc
      121 aagcgaatgg cgaagagaag cagcaggacc ggtccgagcc agaagctcgg cagggcgagc
      181 gctgcggtgg cgctcgcgcc gagagcccga tcgaggcccg aacccggccg cagccccgcc
      241 gcgatcccgg ccgggaggct caggagaagc gccacgagga tcgtcgcccc ggccaggagg
      301 gccgtggcag gcagccgctc ggcgaggagg cgccgcaccg ggtcgccgta gtgaagcgac
      361 aggcccagat cgccccgtcc aatcccgccg agaaagcgca ggtactgctc ggcgagggga
      421 cggtcgaggc cgaggcgggc gcgcaggtcg gcgatgtccc cttcgcgcgc cgccgggccg
      481 agcatcgcct gcaccggatc gccgggaacg aggtgcagga gcaggaacac gatgctggcg
      541 gcgccgagca gcagcgggag cgccagcagt ccccggcgca ggaggtagcc ggtcaaggct
      601 ccgggctggt caaggccttg cggacgagaa gcgccagatc gtgctccaga gtcgtcacgg
      661 gggtctcgac gatgcggccg atttcacgcc cgcgcaccga gaggacgaac gtcgggacga
      721 gctgcagccc gaccccctcg aggcggccgg ccggctcttt cttggtgcga tcgacgccga
      781 cgtagcgcgc cttcaggtgc ccatcccggg ccgcgccgat gatcttcagg aagcggggca
      841 cggcgatcac gctgtccgag caccaggacc cgaaatacac catcaccagc gatccgtccg
      901 gggcgctccg gatgacgtcg atgtcggagg ggtcggggtg atagtcgtcg tggccgcggc
      961 gccactccgg cgcgatgtcg aggatcgccg cgggccggac gcggccgagc acgggatcgt
     1021 gctgcggctc cgacgggacc gccggggacc agggaagcgc cgcgagcgtg gccgcggcgg
     1081 cgaggaaccg gagccggcgc gaccgcatcg aatccgcaag accaccgaat cgtttgacgc
     1141 cgcgcatggc ggcgcccatg atcggggagg cccgcgccgc tgtcaacggt cggcgaacgg
     1201 tccggacgcc ccggcggctc gaatccttga tcccccgatc ggcatctgtt agattcaccg
     1261 gccgcaggag cagccttctg gagggagaaa aacgatgcgg gcgtcatgga caggcttcgg
     1321 agtcgcgttg ctggcagggg cgttgctcct gccgatcgcc acgcccccgc tccccgcggc
     1381 ggcggccgcg gacgatcccg acgccggggc cccggccgcg tcggtcagcg acccgatgct
     1441 ggcgcgcatc ctggagcaat gggacaagcg ccagcaggag acgcagacgc tcgtcgcctc
     1501 cttcaccgag cgcaaggagc tcaacctcct cgccaagccg gtgatctcga agggggagtt
     1561 cttctacagc cgtccgaacc gcgtccgctg ggagtacaag gagccggagc acaaggtctt
     1621 cgtcatcacg gaggacctgt acaccgccta ctatccggcg ctgaagcgcg ccgaggaggt
     1681 gccgatcaag aagttcgtcg gcaagcggct gttccgcttc ctcggagtcg gccagaagat
     1741 cggcgatctg gcccgctatt acgatttcag cctggcgccg acgagcgacg tgaaggggac
     1801 gcacctcctg cgcctgacgc cgcgcaagcg caccgtgcgc gaccgggtct ccgagatgaa
     1861 gatctgggtg gacgacacca ccttcctgcc gcgccagctg cagtacgtcg agtcggacgg
     1921 cgactcgacc ctgctcacct tcgaggggat gcagtcgaat gtcgaggtgg cggcgaaccg
     1981 gttcaaagtg gacctgccgg gggacgtggc ggtctcgcag acgttcaacg gcttcgcgct
     2041 cggccagcag agcttctgac cgccggcacg tcccgcgcca ccccctagtc cgagatgtcg
     2101 atctgaggcc ggaacgagcc gcggttcttc tggttgaggt tgagtgattc ccgcacgtcg
     2161 atcgtcacgg tgaccgtggt ggtcgccccc tcgtcgacac ggacgtcgat cccccgctcg
     2221 atcgtaccgc caagaagcca cggccctgac gcggctctgc gcagtttcag ggatcaggga
     2281 tgagggagcg gagtcaccac ggggcgcagg atgagggtgt tcgagctcgt ctcgttcacc
     2341 tgcaccgagg ccccggcgtc gaagtcgagg gtcagggcgg tcgtctcgtc ctctccgacg
     2401 tggaaccgga tcgggatgtc caccttcccc gattcgatct tcagcggcac tgcctcggtg
     2461 gagtccttgt aggtcagcgt ggcttccgtg acctcgaggc ggatccggtt gtagtcgccg
     2521 gccagcacct ctcccgacgc gagcagcgtc gtggcgccgt cggtgagggt caaaaggttg
     2581 aatgtctctg ggtgggtcaa aaggacgatg gggccgccgt tcaccgaagg catgtcatca
     2641 tcgtcgcctg ggtagacgat gacgtcggtg atcgtcacct cgaccttttc gacggtcgtc
     2701 aggtcgatgg gagcgtcggt caggttcacc tggatgatgc cggtgccgga cccgtcgctg
     2761 tctccgccgc acgagaggag cagtgcgaga cagccagcca gcagcacagt gcgaatcgcg
     2821 caggacgcgg tcatgccaac ctcctcgaca acgccacccc cgggcgagac gtcgatcgta
     2881 gcacggaccg cgaacaatgc ccgtcccagg ccaacaaaaa accgccccgc ggcgcgccgc
     2941 ggggcggtcg gttggacact gtggtcggcc gatgaaaggc cggccgggga ggcctcagta
     3001 catgtcgccg cccatgccgc gcggcccggc ctgggccgcc ttctccttct ccggcagctc
     3061 gtgcaccagc gcctcggtcg tgagcatcag accggcgatg ctggcggcgt tctgcagggc
     3121 ggtgcgggtg accttggtcg ggtcgatgat cccggcctcg aacatgtcca cccacttgcc
     3181 gctgtaggcg tcgaagccgc gcgacgcctt ctcctcctgc ttcgcctggt tcacgatgat
     3241 ggagccctcg aacccggcgt tgccggagat ctgccggagc ggctcctcca gggcccggcg
     3301 tacgatgttg ataccggact ggatgtcctc cgacttctcc ttcagctttt cgatcgccgg
     3361 caccgcgcgc aggagcgcca cgccgccgcc ggggacgatc ccctcctcga cggccgcctt
     3421 ggtggcgtgc atggcatcct cgacgcgcgc cttcttctcc ttcatctcgg tctcggtggc
     3481 cgcgccgacc ttgatgatcg cgacgccgcc gacgagcttg gcgagcctca cctggagctt
     3541 ctcgcggtcg tagtccgagg tggtctcctc gatctgcgcc cggatctgct tgacgcgccc
     3601 ctcgacgtcg gagcgcttcc ccttcccctc gacgagggtg gtgttgtcct tgtcggcggt
     3661 gagcttcttg gcctggccga gatcctcgac cttgatgttc tccagcttga tcccgaggtc
     3721 ctcggtgatg cacttgccgc cggtcaggat ggcgatgtcc tcgagcatcg ccttgcggcg
     3781 atccccgaag cccggcgcct tgaccgcgca gacctggagc gtcccgcgca gcttgttgac
     3841 gaccagggtc gcgagggcct caccctccac gtcctccgcg atgatgagga gcgggcgccc
     3901 gcccttcgcg acctgctcca ggagcggcag cagctcgcgc atgttgctga tcttcttctc
     3961 gtggatcagg atgtaggcgt tctcgaggat gcactccatc cgctcggcgt cggtcacgaa
     4021 gtaggggctc aggtagccgc ggtcgaactg catgccctcg acgatctcga gggaggtctc
     4081 catgccgcgg gcttcctcga ccgtgatgac gccgtccttg ccgaccttct ccatcgcctg
     4141 cgcgatgatc tctccgatgg tctcgtcgtt gttggcggag atggtgccga cctgcgcgat
     4201 cgacttgccg ccgaccgtgc gggacagctt cttgatctcc tccactgcct tctcgaccgc
     4261 ccgatcgatc cccttcttga ggtccatcgg gttggcgccc gcggtgaccg ccttcacgcc
     4321 ctcgcgatag atcgcctggg cgagcacggt ggccgtcgtg gtgccgtcgc cggcgacgtc
     4381 cgaggtcttg ctcgcgacct cgcgcaccag ctgcgcgccc atgttctcgc gcggatcctc
     4441 gagctcgatc tccttcgcga ccgtgacgcc gtccttggtg ctggtcgggg agccgaactt
     4501 cttctcgagg acgacgttgc ggccgcgcgg gccgagggtg accttcaccg cgtccgccag
     4561 cttgttcacg ccgcggagga tcgcctgccg gcactcctcc gaatagatga tttccttggc
     4621 tgccatggtt cgttcgcctc ccttagctga cgatgccgag gatctcgtcc tcgcgcagga
     4681 tgacgtgctc gatgtcgtcg attttgactt ccgtcccgga gtatttgccg accaggatgc
     4741 ggtcgccgac cttcacttcc atcgggatgc gtttgccgtt gtcgtcgaga cggccggagc
     4801 cgaccgagac gaccttcccc tcgaggggct tttctttggc ggtgtccggg atgatgatgc
     4861 cgcccttcac cttctcttcg gcctccatcc ggcgcaccag aacccggtcg ttgagtggct
     4921 ttaccttcat cgggatgacc tcctcctgtg ccttgtctgg gctgggtttg ccagggggcg
     4981 ctgttagcac tctcccctga cgagtgccaa tactaaccac gataaaactg actgtcaagt
     5041 cagggagact cggcttgcgg tccccacagg aggacctatg atttcggcgg gggttcggga
     5101 ggaagattct cccatgatgg tgccaggcag cgcattcgag gatctgactc cggaggcgat
     5161 cgaggccagg ttgcgggccc tcgccgaccg cctggtcggc tcgtcggcct tcgacgaact
     5221 cgacctcagg caccttctgg acctgctgac ccagcgcacc ccgctcctgc gacaggaata
     5281 tgacccgggg accctcgacc gagtcctcgc aggcgatccc cgccccgcgg cgatcctcct
     5341 cccggccgcc ctggccccgg agcatccccg cgacgaggcc gcgctgcggg catggagcgt
     5401 gccggagagt ctgcgcgcct gcggcgacaa atgcctctac gacgtcgggg tggcagggag
     5461 gatgacgtac cgcgggctgc cgctccagga gatcggcccg cgttcctacg acctcgcggg
     5521 ccaggtgctc catctgctcg ccgacgaccg gacgctccgc gagttcttcg agcgcaaccg
     5581 gatccgcaat ctcccgctgg acgaggaggt gctcttcctg cgccagtgcg ccggccgctt
     5641 cgacctgtac gcccgcatcc tgcaggcctg gcgcgaatcg atggggcagg cggcgccggc
     5701 gccggcggaa agggagggga gtccgatcgc ttcgggcagc gcgatcctgc ctgtccctcc
     5761 gccgtcgcgg ccgccgcagc gtcgcagtgc cccgcgcccg aagcgccgcg agacgcagcc
     5821 gccggagccg ccggtcgacg atccggacgg cgaggaggat gggttcaagg acgccgatcg
     5881 ggacgcgcgc ctggcgtggt tcgagcggac gctgctcctg cgcaccctcg acctggagcg
     5941 gctgcgccgg gagctcaggg acgacgtcgt cgatcaggac gaggcggtgc accgcgtgtg
     6001 cgacgacctg agcgtcttcg cggccggcat gcgggagcgc cagcgccccc tgagctattt
     6061 cctcgtcggc ccgaccgggg tagggaagaa ccacctgatg gagtcgctgg cgcggcgcct
     6121 ggaggcgacc tgggagtgcg aggtgccgac gctcatcctg gagggaccgc agtacgccta
     6181 tccgtctgac gtcaacgagc tcaagggggc gacgcgcggc ttcatccgca gcgacgagga
     6241 ggggctcctg gcggagttcc acgagcgcgc ccgccgcgcc cctctttcgg tgctgctggt
     6301 ggacgaggtg gagaaggccc acccgcagct gatgcgcttc ttcctggcgc tcatggatcg
     6361 cggcagcacc atggacaacc gcggccggct cctccggttc ccggcgacca tcctggcatt
     6421 cacgtcgaac gccggctaca gcgagctcga agtgcgccgg ggaccgatcg gctacgcggc
     6481 ggaggaggcg gcaggatcgt cgcggcgccg cgacgcggcg cgggcgctca agcgcgggct
     6541 cgcccccgag ttcctggcgc gggtccaggt gctccacttc cggccgctgg gccgggcctc
     6601 ggcggcccgg atcctcgatc gggagatcgg ccggctggcc cggcggttcc tgcggcgcca
     6661 cggcttgagg ctcgaggtcc tcccggcggc ccgcgcggcg ctcgtggaac agggattcag
     6721 tgtcgaggaa ggggcgcgcc acctcgcggg ccggatcgac cgagtgtgca acgtcgaagt
     6781 gcagctgcgg ctgcgtcccg ccggagtgcc gctgacgtcc gagcgccgcc gcctcctcac
     6841 cctcatccgc gaggcgcgcc gcggcgagcg cgcggtggac gtggaggcgc tgaccgacgc
     6901 ggtgcgtgtg gagctgcgcc cagagaggac cggccggcgc atcgtcatcg actaccggcg
     6961 cgatcgcttc gtctaccgcg aggaagagga gtgaggccgc agccgccgct cgtcccggtg
     7021 ctgcagtcga tcatcgagcg gacctacggg ctcgaacccc tgtacggagg cgtcgagccg
     7081 tttctcatcg gcgacgccgg actgcgcgcg ttccaccgga gggccggggt cggcggcgac
     7141 ctcgagccgg gtcggccgcg cctgatcctg cgatccgagc ggggcggctg gcgggcggtc
     7201 gtctactacc cggacgcgct cgtgcgccac ctcgagcgcc acgatccgcg gcgaggactc
     7261 gacgaccgca acatcgacgc cttcgcgatg ctcgtggagg agctcgatca cctgctggtc
     7321 gtcgccagcc ggctggccgc ggggcggacc ttccggctcg tcgagctgga gatgcacgcc
     7381 ctcgtcacca agtacctcgt ggtgctgcac ctgctgggac gcctgaccgg ccgccggcgc
     7441 gtctcggagt tccaccgcgt ctgggtgcgg catcatctgt tcgagaaata tgcgcgcgcg
     7501 cccgacgggg aggcgcaacg ctatctcgac gcggcccgtc tcgcttggcg ctacgtcgcc
     7561 tggctcgacc ggctcgcggc cgccgcgcgc cacacggagc tgcgtcgttt ccacgcccgg
     7621 ccgttcgagg aacaggcgcg gcgcatcgcg ctgaacggct gaggagaccc cgcctactgg
     7681 ggctgggtgg gacggggagc ccgcggaagc gacgccagct gctgcgcctc tgggaacagc
     7741 tcgatcgccc gctggacctg cttgtcgatc tccatcgcga cgcggtaccc ctcttccatc
     7801 ccccacaggg tcgaggcgat cgcctggcgg atctccgcgc gcaggcggtc ccggtcgacg
     7861 tccacctcgg acttggtcca ggtcaccttg cggcggtcga ggaacttcag gaactcgtcc
     7921 accatctcgg gggtgacgtc caagcccttg gcgagcccct tgtggtgcgt gttgtagtcg
     7981 accgcgaagt tgaagaaggc ctggttgcgc tgcagggtgt cgacgaactt ggaggtcggc
     8041 tcccgatcca ccagcacgtc gggacggatc ccgccgccgc cgtacaccgt ccggccggcg
     8101 tcggtctggc gcacctcgcc gtgcggcggc tcggtggcct cgctgtcttc gtaggagagg
     8161 tagtcctcga gggagttgta gtcgcgctgg atcagacgac cgctcggcgt gtagtagcgc
     8221 gccgtcgtga gcgccagcgc ggttccctgg ctgagcggat agaccgtctg caccagccct
     8281 ttgccccacg tcggcttccc gacgatgagg gcccggtcgt ggtcctggag cgccccggag
     8341 acgatttcgg aggcgctggc gctgaacttg tcgacgaggc acacgatcgg caggtcgggg
     8401 tgccggccgg tgccggacgc ccggaactcc tgatcggatc cgcggatccg gccctgcgtg
     8461 tagaccacca tctgtccctt gcgcaggaac tgctcggaga cccgcaccgc ctgctcgagg
     8521 agcccgcctg gattgcggcg caggtcgagc acgagctgcg tcatcccctc gcgccgcagc
     8581 gcctggatct tctcctccat ctcgcgctcg gtcgtgcggg tgaagttttc gatcttgatg
     8641 tagccgacgc cggggcgcgc catgaaggcg tagcggatgc tgtaggtcgg gatgtcgtcg
     8701 cgc
//
LOCUS       DASRIQ010000025         6815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2616345, whole genome shotgun sequence.
ACCESSION   DASRIQ010000025 DASRIQ010000000
VERSION     DASRIQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2616345"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="VFD06_00670"
     CDS             <1..1503
                     /locus_tag="VFD06_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN02077.1"
                     /translation="ATLGETIAWIIGWDLIIEYAVGNIAVAISWSSYFNDVLRHTIGW
                     TMPSWICTDLRTALRTPEILAAAPHAFGVPIVLNLPAVLIVALVTWVLVIGVKESARV
                     NNLMVALKIVILLLFVAVGCTYVRSDHFTPFFPGGWAGVQAGAALIFFSFIGFDAVST
                     AAEECRDPRRDIPIGILGSLGVCTLLYIAVATVLVGIMPWSDLGVADPLARALAFIGQ
                     DRAAGMVSFGAVVAMTAVLLVFQLGQPRIFFSMSRDGLLPPLFSRVHPRYRTPHIPTI
                     WTGAFVAFFAAFASLDEIIELTNIGTLFAFILVCVGVMVLRYKDPARPRPFRMPWVPL
                     LPIWLLMLIFLPQALLAAEDWGALLEYLILFGIAAVGTAFSISGIAALVRRRPVPEIV
                     KTEFCLAGIASCLYLMRGLPALTWWRFLGWLAIGLFIYAVYGVRHSRLAGTSAATRLA
                     PGMKWLAFATLFAAAACWWRLPHPASLLVPIAMLIGFSAFAIALSRRPAV"
     gene            complement(1481..3211)
                     /locus_tag="VFD06_00675"
     CDS             complement(1481..3211)
                     /locus_tag="VFD06_00675"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZN02078.1"
                     /translation="MTRRMAAAIGLIVAGLAFGSGFAAGPAPAYDLLLRGGRVIDGTG
                     NPWIAADVAVKGDRIAAVGRIDSASARRTIDVKGLVVAPGFIDMLGQSEYTVLIDPFV
                     LGKITQGITTELTGEGGSVGPMTDYSRGEAQAQTRDLGITIDWRDHAGYFRAVERKGT
                     ALNFAHLVGAAQVREAVLQAADRDPGPAELQKMKDLVAQAMRQGAFGLSTSLIYPPGA
                     FAKTDELVALAEVAARHGGFYATHMRTEGAGILDAIEEAATIGERAGCPVEIWHLKAA
                     GRRNWGRMPEILERIETIRARGVDMTADVYPYPAAMTGLAASLPPEASIGGLQALLKR
                     LADPDERTRLRRQMESPGEAFERIFDASGGPEGLLIVGVKTPKNRPLQGKRLSEIAAL
                     RQTDAIDALFDLLLEEGGTIDTVYFSMSEEDVRKAIAAPWVSFDCDASGVRPDGPLAA
                     SMTHPRAYGSFPRILGRYVREARLLRLEEAIRRMTSMPARRIGLVDRGLVRAGLFADL
                     TVFDPEKVIDRATFEAPHQLSEGIVHVLVNGRPVIENGRTTGALPGRILRGPGFAGGK
                     GAAVRPPVGG"
     gene            complement(3208..4512)
                     /locus_tag="VFD06_00680"
     CDS             complement(3208..4512)
                     /locus_tag="VFD06_00680"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZN02079.1"
                     /translation="MSLVAARRHVLGNGLVAIVRPNRATRSIALRLMLRAGSAFDPKG
                     AAGTAHLAARILDRGAGGIPGSRLAEEFDALGVSYDARARLDSLDLTIRCLSRHLPFA
                     LERLHLLASAPDFPEAEVEGERARIQTEIAEREQDTAAQADDLLSGGLFPSGHPYREP
                     LPGTRTSVAALAHEALTAFHRERCGPDGAVLGLAGDFDEREAVERIRRVFEAWGKAAR
                     RSGTPSDGKTPRPALPRAPAPAAASILRKPIEGKTQADVAWGFTPQIERLAPDLQAVM
                     VMNTVLGDFGMGGRIGRSVREEAGLAYHASSYVWCGLTAGPLVVRAGVAPEGLKKAIA
                     LMRRTVREFLRRGPLPRELQDAKQALAAAIPRRFETNLGAAALLADSEFQGLGFDFAD
                     RAPALIARVDRGAAHEAARKYVTPDRSVLVVTGPDIPEKDLR"
     gene            complement(4509..5783)
                     /locus_tag="VFD06_00685"
     CDS             complement(4509..5783)
                     /locus_tag="VFD06_00685"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZN02080.1"
                     /translation="MSVRPRTATLANGLRVLLHPMPRTGLVSVWCWYRVGSKDERPGV
                     TGISHWAEHMNFKGTRRYGRDEITRRVELAGGLWNGYTWLDVTTYFETVQASAFEEML
                     RIEASRMTECLYEPKEVERERTVVISELQGGENDPRTYLEREVSGTALQAHPYRWPTI
                     GYLSDLRAITREDLYRHYRAYYVPNNAILVASGDFQPGRALALVRRHFGRLKRGPDVP
                     AVRTLEPDQSGERRVNVRRPSGASYLHAAWPAPAAAEKDFAALLVVDGLLAGGPSVNA
                     WSGAGRPPSKASRLYRALIDTGLAAEFGTQSIPTRHPYLYTVQATAREGVDLERLEAA
                     ALAEIAAVAQGRFAAADLERAKEQFLARHALESESVTDVAHQLGFFETIGGHALWLDL
                     PRRVAAVTAEDAARLAGARLDERHRTVGWLRP"
     gene            complement(5780..>6815)
                     /locus_tag="VFD06_00690"
     CDS             complement(5780..>6815)
                     /locus_tag="VFD06_00690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN02081.1"
                     /translation="IAVHWGLIFFTRTVWQVLALRIMLGLFSGFGTMSVALVTHGCPR
                     GRIGHAVGTLQATQIMSTAIGPFVGGLLAAGIGIRNTYLVTCTLCTVAFLFVLLLYRD
                     IPASEATEAPMVVGQAGPVSEGWRAILPRRLRGPRAAKPSFAAILRLPLLLPLLPLLF
                     LVNMVDRALFLIVPLCVAAPGMHVRSLEATTGIVMSGGAFAASASAYVFGRIAGRRVH
                     ATRLLLVSLLAGAVLIVVMALAHDVLAFTVSRILLGLAVGGAPTLAYAIAGDHIPDTV
                     RASSYALLSSTAMLGASMGPTMTGVLSTANLRAPLWAGGATYLLLAAQTVALDRRRRS
                     HAAALAEAGT"
BASE COUNT          907 a         2464 c         2452 g          992 t
ORIGIN      
        1 gccacgctcg gcgagacgat cgcctggatc atcggctggg acctgatcat cgaatatgcc
       61 gtcggcaaca tcgccgtggc gatctcctgg tcgtcctact tcaacgacgt cctccggcac
      121 acgatcggct ggaccatgcc gtcctggatc tgcaccgacc tgcggacggc gctccggacg
      181 ccggagattc tcgcggcagc gccgcacgcg ttcggcgtgc cgatcgtgct gaacctgccg
      241 gcggtgctga tcgtcgccct ggtgacctgg gtcctggtga tcggcgtcaa ggagagcgcc
      301 cgcgtcaaca acctgatggt ggctctcaag atcgtcatcc tgctgctgtt cgtcgcggtc
      361 gggtgcacct acgtccggtc cgaccacttc acgccgttct tcccgggcgg ctgggccggc
      421 gtgcaggcgg gggcggcgct gatcttcttc tcgttcatcg gcttcgacgc cgtgtccacc
      481 gccgccgagg agtgccgcga cccgcggcgc gacatcccga tcggcatcct cggatcgctc
      541 ggcgtctgca ccctcctcta catcgcggtc gccacggtgc tcgtcggcat catgccgtgg
      601 agcgatctcg gcgtcgccga tccgctcgcc cgggcgctcg ccttcatcgg ccaggaccgg
      661 gccgccggca tggtgtcgtt cggggcggtt gtggcgatga ccgccgtgct gctcgtcttc
      721 cagctggggc agccgcgcat cttcttctcg atgtcgcgcg acggcctgtt gccgccgttg
      781 ttctcgaggg tgcatccgcg ttaccgcacc ccgcatatcc cgaccatctg gaccggggcg
      841 ttcgtggcgt tcttcgccgc cttcgcctcg ctcgacgaga tcatcgagct gaccaacatc
      901 gggacgctgt tcgccttcat cctggtctgc gtcggcgtca tggtcctgcg ctacaaggat
      961 ccggcgcggc cgcgcccgtt ccgcatgccg tgggtgccgc tcctgccgat ctggctgctc
     1021 atgctcatct tcctgccgca ggcgctcctc gcggcggagg actggggggc gctgctggag
     1081 tacctgatcc tcttcggcat cgcggcggtc ggcaccgcct tctcgatcag cgggatcgcg
     1141 gctctcgtgc gccggcgtcc ggttccggag atcgtcaaga ccgagttctg tctcgccggg
     1201 atcgcctcct gcctctacct gatgcgcggt ctgccggcgc tcacctggtg gcgcttcctc
     1261 gggtggctgg ccatcgggct gttcatctac gcggtgtacg gcgtgcggca cagccggctg
     1321 gcgggaacga gcgccgcgac gcgcctcgcg ccggggatga agtggctcgc cttcgccacg
     1381 ctgttcgccg cggccgcctg ctggtggcgc ctgccgcacc cggcgtcgct gctcgtgccg
     1441 atcgcgatgc tgattggatt ctcggcgttc gcgatcgcgc tcagccgccg accggcggtc
     1501 tgaccgccgc gccctttccg ccggcgaagc cgggcccgcg caggatccgt cccggcagcg
     1561 cgccggtcgt ccggccgttc tcgatcaccg gccgtccgtt gacgaggacg tgcacgatcc
     1621 cctccgacag ctggtgcggt gcctcgaagg tggcgcggtc gatcaccttc tccggatcga
     1681 agacggtcag gtcggcgaag agccccgcgc ggacgaggcc gcgatcgacg aggccgatgc
     1741 gccgcgccgg catcgaggtc atccggcgga tcgcctcctc cagccgcagc aggcgcgctt
     1801 cgcgcacgta gcgtccgagg atccgcggga acgacccgta ggcgcgcggg tgcgtcatcg
     1861 acgctgcgag ggggccgtcg ggacggacgc cggaggcgtc gcagtcgaac gacacccacg
     1921 gcgccgcgat cgccttgcgc acgtcctcct cggacatcga gaaatacacc gtgtcgatcg
     1981 tccccccctc ctcgagcagg agatcgaaca gcgcgtcgat ggcgtccgtc tgccgcagcg
     2041 ccgcgatctc cgacaggcgc ttcccctgga gcggtcggtt cttcggggtc ttcaccccga
     2101 cgatcaggag cccctccggc ccgcccgagg cgtcgaagat ccgctcgaac gcctctccgg
     2161 ggctttccat ctgccggcgc agccgggtcc gctcgtccgg atcggcgaga cgcttgagga
     2221 gcgcctggag ccctccgatc gacgcctccg gcggcagcga ggcggccaga ccggtcatgg
     2281 cggcgggata gggatagacg tcggcggtca tgtccacgcc gcgcgcgcgg atcgtctcga
     2341 tccgctcgag gatctccggc atgcgccccc agttgcgccg ccccgcggcc ttgaggtgcc
     2401 agatctccac cgggcacccg gcgcgctccc cgatggtcgc cgcctcctcg atggcgtcga
     2461 gaatcccggc cccctcggtt ctcatgtgcg tggcgtagaa gccgccgtgg cgcgccgcca
     2521 cctccgccag cgccaccagc tcgtccgtct tcgcgaacgc gccgggcggg tagatgaggg
     2581 aggtggacag cccgaaggcc ccctggcgca tcgcctgggc gacgaggtct ttcatcttct
     2641 ggagctcggc cgggcccgga tcgcggtcgg cggcctgcag gaccgcctcg cgcacctgcg
     2701 ccgcgccgac gaggtgcgcg aagttgaggg ccgttccctt gcgctccacg gcgcggaagt
     2761 agccggcgtg atcgcgccag tcgatggtga tgccgaggtc gcgcgtctgg gcctgggctt
     2821 ctccccggct gtagtcggtc atcggtccga ccgagccgcc ttcccccgtc agctcggtcg
     2881 tgatcccctg cgtgatcttc cccaggacga acggatcgat gaggaccgtg tactcggact
     2941 ggcccagcat gtcgatgaag ccgggggcga cgacgagtcc cttcacgtcg atcgtccggc
     3001 gggcggaggc ggagtcgatc cggccgacgg cggcgatccg gtcccccttc accgcgacgt
     3061 ccgccgcgat ccaggggttg ccggtcccgt cgatgacgcg gccgccgcgc aggaggaggt
     3121 cgtaggccgg cgcgggtccg gcggcgaacc ccgagccgaa cgccagaccg gcgacgatga
     3181 gaccgatcgc cgcggccatc cggcgggtca tcgcaggtct ttttcgggga tgtcaggccc
     3241 ggtgaccacg aggaccgagc gatcgggggt gacgtacttt ctggccgcct cgtgcgcggc
     3301 cccccggtcc acgcgggcga tgagggccgg ggcccgatcg gcgaagtcga agccgagtcc
     3361 ctggaactcg gaatcggcga gcagcgccgc cgcccccagg ttcgtctcga agcggcgcgg
     3421 gatggcggcg gcgagcgcct gcttcgcgtc ctggagctct cgaggcagag gtccccggcg
     3481 caggaactcg cgcaccgtgc ggcgcatcag cgcgatcgcc ttcttcagcc cttcgggggc
     3541 gacgccggcg cgcaccacga gcggtccggc ggtcaggccg caccagacgt acgacgaggc
     3601 gtggtacgcc agccccgcct cctcgcggac gctccggccg atccgtcccc ccatgccgaa
     3661 gtcgccgagg acggtgttca tcaccatcac cgcctggaga tcgggggcga gccgctcgat
     3721 ctgcggggtg aagccccagg cgacgtcggc ctgcgtcttc ccctcgatcg gcttccggag
     3781 gatggaggcg gcggccggag cgggggcgcg cgggagcgcg gggcgcggag tctttccgtc
     3841 ggacggagtt ccggaccgcc gcgccgcctt cccccaggct tcgaagacgc ggcggatccg
     3901 ctcgacggcc tcccgctcgt cgaaatcgcc ggcgaggccg agcaccgccc cgtccgggcc
     3961 gcagcgctcc cggtggaagg cggtcagggc ttcgtgtgcc agcgccgcga cgctcgtccg
     4021 cgtgccgggg agcggctccc ggtacgggtg gccggagggg aagaggccgc cggagaggag
     4081 atcgtccgcc tgcgccgcgg tgtcctgctc ccgctcggcg atctcggtct ggatgcgggc
     4141 ccgctcgccc tccacctccg cctccgggaa gtcgggggcg ctcgccagga ggtgcaggcg
     4201 ctcgagggcg aacggcaggt gccgcgacag gcagcggatc gtcaggtcga ggctgtcgag
     4261 ccgggcgcgg gcgtcgtagg agacgccgag cgcgtcgaac tcctccgcca gccggctccc
     4321 gggaatgccg ccggcgccgc gatcgagaat gcgcgccgcc aggtgcgccg tcccggcggc
     4381 gcccttcgga tcgaaggccg agcccgcccg gagcatcagc cgcagcgcga tcgagcgcgt
     4441 cgctcggttc ggccgcacga tcgcgaccag accattgccg aggacgtgcc ggcgggccgc
     4501 gaccaggctc acgggcgcag ccatccgacc gtccggtgcc gctcgtcgag ccgcgcgccg
     4561 gcgagccgcg ccgcgtcctc cgcggtcacc gccgccacgc ggcgggggag atcgagccac
     4621 agggcgtggc cgccgatcgt ctcgaagaac ccgagctggt gcgccacgtc ggtgacgctc
     4681 tcgctctcga gcgcgtgccg cgccaggaac tgctccttgg cgcgctcgag gtccgcggcg
     4741 gcgaagcgcc cctgcgccac cgccgcgatc tcggcgaggg ccgccgcctc gagccgttcg
     4801 agatcgaccc cttcgcgcgc cgtcgcctgc accgtgtaca ggtagggatg gcgcgtcggg
     4861 atcgactggg ttccgaactc ggccgcgagc ccggtgtcga tgagggcgcg gtacaggcgc
     4921 gacgccttgc tcggaggccg acccgccccc gaccaggcgt tgacgctcgg cccgccggcg
     4981 agcaggccgt ccacgacgag gagcgccgcg aagtccttct cggccgccgc cggggcgggc
     5041 caggcggcgt gcaggtacga cgcgccggac gggcgccgga cgttcactcg ccgctcgccc
     5101 gactggtccg gctcgagggt gcgcacggcc ggcacgtcgg ggccgcgctt gagccggccg
     5161 aagtgccggc gcacgagcgc gagcgcccgc cccggctgga agtcgccgct cgcgacaagg
     5221 atggcgttgt tcggcacgta gtaggcgcgg taatggcggt acaggtcctc gcgcgtgatg
     5281 gcgcgcagat cggacaggta cccgatcgtc ggccagcggt acggatgcgc ctggagcgct
     5341 gtcccggaaa cctcgcgctc gaggtaggtg cgcggatcgt tttccccgcc ctgcagctcg
     5401 gagatcacga cggtgcgctc gcgctccacc tccttcggct cgtacaggca ctcggtcatg
     5461 cgcgaggcct cgatgcgcag catctcttcg aacgcggagg cctgcaccgt ctcgaagtac
     5521 gtcgtgacgt cgagccaggt gtagccgttc cagagcccgc ccgccagctc gacccgccgc
     5581 gtgatctcgt cccggccgta gcggcgcgtc cccttgaagt tcatgtgctc cgcccagtgc
     5641 gagatgccag tcaccccggg gcgctcgtcc ttcgagccga cccggtacca gcaccagacc
     5701 gacacgagcc cggtgcgcgg catcgggtgc aggaggaccc gcaggccgtt ggcgagggtg
     5761 gccgtgcgcg ggcggacgct catgtgccgg cctccgcgag ggcggccgcg tgtgatcggc
     5821 ggcggcggtc gagggcgacg gtctgcgccg cgagcaggag ataggtggcc cccccggccc
     5881 agagcggcgc gcgcaggttc gcagtgctga gcacgccggt catggtcggc cccatcgacg
     5941 cccccagcat ggcggtgctg gagagcaggg cgtacgacga ggcgcgcacc gtgtcgggga
     6001 tgtgatcgcc ggcgatggcg taggcgagcg tcggggcgcc gccgaccgcg aggccgagca
     6061 ggatgcgcga caccgtgaag gcgaggacgt catgcgccag cgccatgacg acgatgagca
     6121 ccgccccggc cagcaggctg accaggagga ggcgcgtggc gtggacccgg cgcccggcga
     6181 tgcgcccgaa cacgtacgca gacgcggacg ccgcaaacgc cccgcccgac atcacgatcc
     6241 cggtcgtcgc ctcgagcgag cggacgtgca tccccggcgc cgcgacgcac agcggcacga
     6301 tcaggaacag ggcgcggtcg accatgttca cgaggaacag gagcggcaga agtggcagca
     6361 gcagcggcag ccggaggatc gccgcgaacg acggcttggc ggcgcgcggc ccgcgcagcc
     6421 ggcgcggcag gatggcgcgc cagccttcgg agacgggccc ggcctgcccc acgaccatcg
     6481 gcgcctcggt cgcctcggac gccgggatgt cgcggtacag cagcaggacg aaaaggaacg
     6541 ccacagtgca gagcgtgcac gtcacgaggt acgtgttgcg gatgccgatc ccggccgcca
     6601 gcaggccgcc gacgaacgga ccgatggcag tgctcatgat ctgcgtcgcc tgcagcgtgc
     6661 cgacggcgtg gccgatgcga ccgcgcgggc agccgtgggt cacgagcgcc accgacatcg
     6721 tgccgaagcc cgagaagagc cccagcatga tccgcagcgc cagcacctgc cagacggtgc
     6781 gggtgaagaa tatcagcccc cagtgcaccg cgatg
//
LOCUS       DASRIQ010000026         6273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3132599, whole genome shotgun sequence.
ACCESSION   DASRIQ010000026 DASRIQ010000000
VERSION     DASRIQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132599"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /gene="rfaE1"
                     /locus_tag="VFD06_00695"
     CDS             <1..1142
                     /gene="rfaE1"
                     /locus_tag="VFD06_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HZN02082.1"
                     /translation="AGRGGHDRAGGSEGRLSRHGPASARLSAARAREIADRFSAAQVA
                     VVGDLMIDRFIWGRVSRISPEAPVPIVREERRSASLGGAGNVARNVQALGGRVSLAGL
                     VGDDADGRDLSRLCSEAGLAADGIVAVPGRPTTVKTRIVAHHQHVVRLDREEDGSAPE
                     AAGSALRARALEAVAAAQVLVVSDYDKGAIGPDLLGAVLAEASRRGLPVVVDPKTRLM
                     DHYRPATIVTPNARETAEAWGRPVRTDADLEEAGRALLRRLSSPWLLVTRGERGMLLL
                     PARGETIEVPARAREVFDVSGAGDTVVATLALALASGAEMVEAVILANHAAGVVVGKL
                     GTAALDREELLRAIETDGPANRPTGAARSPASGPPTGTGTARNRP"
     gene            complement(1063..1794)
                     /locus_tag="VFD06_00700"
     CDS             complement(1063..1794)
                     /locus_tag="VFD06_00700"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZN02083.1"
                     /translation="MQQAKAHVREGVLSSATFPALLYSILRRSDTGVLTLTGEAAEKT
                     IYVQDGRPIFATSSDRDDRLGQILFKNGAVSLEGLMNAVETSIGHGKRLGTVLVEEGL
                     IQPHDLVEGVRSQVRHIINSLFVWTRGRYRYSPGDLPTDEVITLKLSPGDIILDGIRT
                     IESWGRIWEAVGPLGAIYRTTDRLESLVPELSLSLEQWSLLSHCERPTSLKELCRLSP
                     MADFEQCRFLWAALTLGIVQRQSGG"
     gene            2031..3224
                     /locus_tag="VFD06_00705"
     CDS             2031..3224
                     /locus_tag="VFD06_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02084.1"
                     /translation="MELDGEIRGSQRLGNYLRRLRTGYGLSLRRVEDKARQEGGEIDN
                     SQLSRYERGICYPSFDKLCLLANIFNVSIQNFSDLLELEKVEAFEPEESAGYEALRAE
                     GNREWEQGNFARAYSVYEKALARLESGAEESVPSDVLARARFNLARSLLRMGKISLAE
                     TELRIILRRHREIAPETTALVLLCLADAHADKGDAYLAVLEAEKSLEIARTAGNLEYE
                     GYALHRVARIQFEQGEFERALESFREARTRLEPVVTKHDLTLIRTNIGLCHARVGKVD
                     RGLRELSEALQVAQREGYRRCAAYALLYMGQVHEMRHDEKKARDLFEQAEMMAHGGEE
                     RYVDILFQTAYHLWDMARTAGNQVQEKVYFGRLKFLRPQLDRRFAEVEQFDRFIESGK
                     GVVHA"
     gene            3221..3961
                     /locus_tag="VFD06_00710"
     CDS             3221..3961
                     /locus_tag="VFD06_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02085.1"
                     /translation="MKRIAIVSTLALALLTPLAARAEVSVQVNPDGKVTRIIVLSKGG
                     SGRIWRQVRGHVPAVLLLNPLGDTYGDLAPSVAMNPKTGQPWAIWPQNEGNRKRLVAS
                     FWNGSAWSDPVRIAQSDLMGYDQTEPRLIFDAAGVPYVFFTDGEHDRRVLFVTLNDRG
                     WTPPLRLSDVGVDSSAPSAVLKGSDVRITFKTPAGSLTRTMSTTFLAESAYNLMDSPI
                     PPGYMPVPPPPPGAPTEEPGGSDTIFRK"
     gene            complement(3995..4336)
                     /locus_tag="VFD06_00715"
     CDS             complement(3995..4336)
                     /locus_tag="VFD06_00715"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HZN02086.1"
                     /translation="MNILVYLLVCLFFILLFVLGIYNTEDVTVNLILWRAGPAPMGAV
                     IATAAILGIAFACAIGILDGIKIRIANRQLRRQLQKLDEECDALRLQLARHQGPTAAP
                     PDGEKEKARSY"
     gene            complement(4333..4836)
                     /locus_tag="VFD06_00720"
     CDS             complement(4333..4836)
                     /locus_tag="VFD06_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN02087.1"
                     /translation="MALVLMDVDGVLTDGRLGLGENGREARSWHVRDGSGIVLARRAG
                     LRTGFLSGRGDAGVRRRARELGVDEVHLRARDKGAVYGEMVRRLGLDHKDVCYIGDDL
                     VDLPVLSRVGVPVAVADAHPEVRRRVPFVTSAPGGRGAVREVIDALLQAQGRMREILS
                     WFEGGAR"
     gene            complement(4886..5842)
                     /locus_tag="VFD06_00725"
     CDS             complement(4886..5842)
                     /locus_tag="VFD06_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZN02088.1"
                     /translation="MPKDIARKVLELEAQAILDLVPRLGEGFERAVEILSACRGRVIV
                     TGMGKSGLVGAKIAATFSSTGTPSIFLHPAEAVHGDIGMVVPGDVVLAISSSGETEEL
                     VRLLEILRRLEVGLVALTGGRDSTLARHAQAAIDVGIEREASPLELVPTASTTAAMAM
                     GDALAMALAERRGFTLEDFARNHPGGRLGRKVLTVGHLMHAGSAAPLVQAGAPLSEAL
                     RVMSDGKMGMAAIVSRGDVLEGVVTDGDLRRALLRGADLHRMVVDEAMSRTPVTIAKG
                     DLAAAALSLMEARKITSLVVVGAGRRVEGVLHLHDLWRTQLF"
     gene            complement(5843..>6273)
                     /locus_tag="VFD06_00730"
     CDS             complement(5843..>6273)
                     /locus_tag="VFD06_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZN02089.1"
                     /translation="VNIKKGQFLAPWDIRLAADKVASTGNDKILLTERGSSFGYKNLV
                     VDFRSLAIMRGLGWPVVMDATHAVQLPGGEGDRSGGEAQFIPFLARAAAATGIDALFC
                     EVHDDPASAKSDGPNALRLELVESLLDQVRRIDGVVRETS"
BASE COUNT          968 a         2186 c         2229 g          890 t
ORIGIN      
        1 atgctggtcg aggaggccac gatcgagccg gagggtcgga gggacgcctg agccgtcacg
       61 gcccggcgag cgcgcgcctc tccgccgccc gggcccggga gatcgccgac cggttctccg
      121 ccgcgcaggt cgccgtggtg ggggacctga tgatcgatcg cttcatctgg ggacgcgtca
      181 gccggatctc ccccgaagcc ccggtgccga tcgtccgcga ggagcgccgc agcgcgtcgc
      241 tcggcggcgc cgggaacgtc gcccgcaacg tccaggcgct cggaggccga gtgtccctcg
      301 cgggtctcgt gggggacgac gccgacgggc gcgacctctc ccgactgtgc agcgaggcgg
      361 ggctcgccgc cgacgggatc gtggcggtcc cgggccggcc gacgacggtc aagacccgca
      421 tcgtggcgca ccaccagcac gtcgtccgac tcgaccgcga ggaggacggc tccgcgcccg
      481 aagcggccgg gagtgcgctt cgcgcccggg cgctcgaggc ggtcgccgcg gcgcaggtgc
      541 tcgtcgtctc cgactacgac aagggagcga tcggccccga cctcctcggc gcggtcctgg
      601 cggaggcgtc gcgtcgcggc ctgcctgtcg tcgtcgatcc caagacgcgt ctcatggatc
      661 actaccggcc ggccaccatc gtcacgccga acgcgcgcga gacggcggag gcctggggcc
      721 ggccggtgcg gaccgacgcc gacctcgaag aggccggtcg cgcgctgctg cggcggctct
      781 catcgccgtg gctcctggtc acacgggggg agcgcggcat gctgctcctt cccgcgcggg
      841 gtgagacgat cgaggtcccg gcgcgggcgc gcgaggtgtt cgacgtcagc ggggcggggg
      901 acaccgtcgt ggcgacgctc gcgctggcgc tggcatcggg cgcggagatg gtcgaggcgg
      961 tgatcctggc gaatcacgcg gcgggtgtgg tcgtcggcaa gctcgggacc gccgccctgg
     1021 accgcgagga gctgctccgg gcgatcgaga ccgacggacc ggctaaccgc ccgactggcg
     1081 ctgcacgatc cccagcgtca gggccgccca caggaaccgg cactgctcga aatcggccat
     1141 aggggagagc cggcagagct ccttcaggct ggtcggccgc tcgcagtggg agagaaggct
     1201 ccactgctcg agcgagagcg acagctccgg caccagcgac tcgagacggt cggtcgtgcg
     1261 gtagatcgcg ccgagcggcc ccaccgcctc ccagatccgc ccccagctct cgatcgtgcg
     1321 gattccatcg aggatgatgt cgccggggct cagcttgagg gtgatgacct cgtcggtcgg
     1381 caggtcgccc ggagagtagc ggtaccggcc gcgcgtccag acgaacaggc tgttgatgat
     1441 gtgccggacc tgggagcgga ccccctccac caggtcgtgc ggctggatca ggccttcctc
     1501 aaccaggacg gtcccgaggc gcttgccgtg gccgatcgat gtctcgaccg cgttcatcag
     1561 gccttcgagc gacacggcac cgttcttgaa gaggatctgg ccgagccggt cgtcccggtc
     1621 gctcgaggtg gcgaagatcg gccggccatc ctggacgtag atggtctttt cggccgcctc
     1681 gcccgtcagg gtcaggacgc cggtgtcgct ccggcggagg atggagtaga ggagcgccgg
     1741 gaaggtggcg ctcgacagaa ctccttcgcg gacgtgcgcc ttggcctgct gcatcgcctc
     1801 ctcgggcggg tgggaacgta gaagcccccc cgggggatgt caaggcggcc cggggggcaa
     1861 gaatgcttcg aaggggatgc tgcactcgcc ccgcgatggg attacaatcc tatcttgaca
     1921 gggcgggccc atcaccgcac ccttccgggc aattcgcccc atccccccgg ccggtgggtt
     1981 tggggcacgg tggtccggaa ttcgcccgag ggcagcggag gtcgaggacg atggaacttg
     2041 atggcgaaat tcgaggcagc caaagactcg gcaactacct gaggaggctg cggaccggct
     2101 acggcctttc cctccgtcgc gtggaggaca aagcccggca ggaggggggc gagatcgaca
     2161 actcccagct gtcgcggtac gagcgcggga tctgctaccc ctcgttcgac aagctgtgcc
     2221 tcctggccaa catcttcaac gtttccatcc agaacttctc cgatctcctc gagctcgaga
     2281 aggtcgaggc gttcgagccg gaggagtcgg ccgggtacga ggcgctccgg gccgagggga
     2341 accgcgagtg ggagcagggg aatttcgccc gcgcctactc ggtgtacgag aaggcgctgg
     2401 cgcgcctgga gtcgggggcc gaagagtcgg tcccgtcgga cgtcctcgcc cgcgcccgct
     2461 tcaacctggc ccgctcgctc ctgcgcatgg gaaagatcag cctggcggag acggagctgc
     2521 ggatcatcct ccggcggcac cgggagatcg cccccgagac cacggcgctc gtcctgctgt
     2581 gcctcgcgga cgcgcacgcc gacaaggggg atgcgtacct cgccgtgctc gaggcggaga
     2641 agagcctgga gatcgcccgg acggccggga acctggaata cgaagggtac gcgctgcacc
     2701 gcgtggcgcg cattcagttc gagcaggggg agttcgagcg ggcgctggaa agcttccgcg
     2761 aggcccgcac ccggctcgag ccggtggtga cgaagcacga cctgaccctc atccgcacca
     2821 atatcgggct ctgccacgcg cgcgtcggca aggtggaccg cggcctccgc gagctgtccg
     2881 aggcgctgca ggtcgcgcag cgcgagggat accgtcgctg cgccgcctat gccctcctgt
     2941 acatggggca ggtgcacgag atgcgccacg acgagaagaa ggcccgcgac ctgttcgagc
     3001 aggccgagat gatggcgcac ggtggcgagg agcgttacgt cgacatcctg ttccagaccg
     3061 cgtaccacct ctgggacatg gcccgcaccg ccggcaatca ggtgcaggag aaggtgtact
     3121 tcggccgtct caagttcctc aggccgcagc tggaccgcag gttcgccgag gtggagcagt
     3181 tcgaccgctt catcgaaagc gggaagggag tggtgcacgc atgaagcgga tcgcgatcgt
     3241 ctccactctg gcgctggccc tcctcacgcc cctcgcggcg cgcgcggagg tttcggtgca
     3301 ggtgaacccc gacggcaagg tcacgcgcat catcgtcttg tcgaaggggg gatcgggacg
     3361 catctggcgg caggtgcgcg gacacgtccc ggcggtgttg ctcctcaacc cgctggggga
     3421 cacgtacggc gacctcgccc cctctgtcgc gatgaacccg aagaccgggc agccgtgggc
     3481 gatctggccg cagaacgagg ggaaccgcaa gcggctggtg gcgtccttct ggaacggctc
     3541 cgcctggagc gatccggtgc gcatcgccca aagcgacctc atggggtacg accagaccga
     3601 gccgcggctg atcttcgatg cggctggcgt tccctacgtc ttcttcaccg atggggagca
     3661 cgatcggcgc gtcctgttcg tgacgctgaa cgaccgcggc tggacgccgc cgctgcggct
     3721 gagcgacgtc ggcgtggaca gcagcgcccc gtcggcggtg ctcaaaggga gcgatgtccg
     3781 gatcacgttc aagacgcccg ccggttccct gacccgcacg atgtcgacga ccttcctggc
     3841 ggagagcgcg tacaacctga tggacagccc gatcccgccg ggctacatgc cggtgccgcc
     3901 tcccccgccc ggcgctccga ccgaggagcc gggggggagc gacaccatct tccggaagta
     3961 agcggaacag tcgaggaaag gattcaaggc ccggtcagta cgaccgggcc ttttcctttt
     4021 cgccgtccgg cggtgcggcg gtcggtccct ggtgccgggc gagctgcagc cgcagcgcgt
     4081 cgcactcctc gtcgagcttc tgcagctggc gccgcagctg ccggttggcg atgcggatct
     4141 tgatcccgtc gaggatgccg atggcgcagg cgaaggcgat cccgaggatg gcggcggtgg
     4201 cgatcaccgc ccccatcggg gccggaccgg cgcgccagag gataaggttc acggtcacat
     4261 cctcggtgtt gtagatgccg aggacgaaca gcaggatgaa gaagaggcag acgagcaggt
     4321 agaccaggat gttcatcgcg cccccccctc gaaccaggac aggatctccc gcatgcggcc
     4381 ctgcgcctgg aggagcgcgt cgatcacctc ccgcaccgcc ccgcggccgc ccggggcgga
     4441 ggtgacgaag gggacgcgcc ggcggacctc cggatgggcg tcggccaccg ccaccggcac
     4501 gcccacccgg ctcagcaccg gcaggtcgac gagatcgtcg ccgatgtagc agacgtcctt
     4561 gtgatcgagc ccgagccgcc gcaccatctc cccgtacacc gcccccttgt cgcgggcgcg
     4621 caggtgcact tcgtccaccc ccagttcacg cgcccgccgg cgcacgccgg cgtcgccgcg
     4681 cccggagaga aacccggtgc gcaggccggc gcgccgcgcc agcacgattc ccgacccgtc
     4741 gcgcacgtgc caggagcgcg cctcgcgccc gttctccccg aggccgagcc ggccgtcggt
     4801 gagcacgccg tcgacgtcca tcaggaccag agccacgcgc gccgcgcgcg gccggaatgg
     4861 cctccggcag atcaacgcat cccccctaga acagctgcgt ccgccacaga tcgtgcaggt
     4921 gcagcacccc ttccaccctg cgcccggcgc cgacgacgac gagcgaggtg atcttgcgcg
     4981 cctccatgag gctcagggcg gcagcggcga gatccccctt cgcgatagtg accggagtcc
     5041 tggacatcgc ctcgtcgacg accatccggt gaagatcggc gccgcgcagg agcgcgcgtc
     5101 tgaggtcgcc gtcggtgacg acgccttcga gaacgtcgcc gcgggacacg atcgcggcca
     5161 ttcccatctt gccgtcgctc atcacccgga gtgcctcgga gagcggagcg cccgcctgca
     5221 ccaggggggc cgcgctcccc gcgtgcatca gatggccgac cgtcagcacc ttgcgcccca
     5281 gccggccgcc cggatggttg cgggcgaaat cctcgagggt gaagccgcgg cgctcggcga
     5341 gtgccatcgc cagcgcgtct cccatcgcca tcgccgccgt cgtgctggcg gtcggcacga
     5401 gctccagcgg ggaggcctcc cgctcgatgc cgacgtcgat cgccgcctgg gcgtgccgcg
     5461 cgagcgtcga gtcgcgacct ccggtgaggg ccacgagccc gacctccagg cgccgcagga
     5521 tctcgagcag gcgcaccagc tcctcggtct cgccgctcga ggagatggcc agcaccacgt
     5581 cccccggcac caccatgccg atgtcgccgt gcaccgcctc ggccgggtgc aggaagatcg
     5641 acggggtccc ggtgctggag aaggtggcgg cgatcttcgc gccgacgagg cccgatttcc
     5701 ccatcccggt cacgatcacc cggccgcggc aggcggacag gatctcgacc gcgcgctcga
     5761 acccttcgcc gaggcgcggg acgagatcga ggatcgcctg cgcctcgagc tcgaggactt
     5821 tccgggcgat gtctttgggc acctaggagg tctcccggac cacgccgtcg atccgccgca
     5881 cctggtcgag cagcgactcg accagttcga gccggagcgc gttcggcccg tcgctcttcg
     5941 ccgatgcggg gtcgtcgtga acctcgcaga agagcgcgtc gatcccggtc gcggccgcgg
     6001 cccgcgccag gaacgggatg aactgcgcct cgcccccgga acgatcccct tccccgccgg
     6061 ggagctgcac ggcgtgcgtc gcgtccatca ccaccggcca gccgagcccg cgcatgatgg
     6121 cgagggagcg gaagtccacc acgaggttct tgtagccgaa cgaggagccg cgctcggtga
     6181 gcaggatctt gtcgttgccg gtggaggcga ccttgtcggc cgcgagacgg atgtcccacg
     6241 gtgcgaggaa ctgccccttc ttgatgttca ccg
//
LOCUS       DASRIQ010000027         5132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1829993, whole genome shotgun sequence.
ACCESSION   DASRIQ010000027 DASRIQ010000000
VERSION     DASRIQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829993"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..2762
                     /locus_tag="VFD06_00735"
     CDS             300..2762
                     /locus_tag="VFD06_00735"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZN02090.1"
                     /translation="MSLVRTILRRPEGMFLPAVLALLLLSLAPGAALAQEGDEVAAHR
                     GGEASLVLPDVGQAEFLGMNGRSLLLGGLLVCVLGLGFGLMMLAQVKAAPVHRSMAEI
                     SELIYETCKTYLITQGKFILILEVFIGIIILLYYGVLQHFAAMKVAIILIFSLIGIGG
                     SYGVAWFGIRMNTFANSRTAFASLRGRPFPLYSIPLKAGMSIGMLLISVELFMMLCIL
                     LFIPGDYAGPCFIGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNP
                     GVIADCTGDNAGDSIGPSADGFETYGVTGVALISFILVAVPEALTQVQLLVWIFMMRI
                     LMIIASGLSYVLNEAWARSAYGNATKMNFEKPLTTLVWLTSVVSMAMTFIASYLLIPD
                     LGDGTLWWKLSLVITCGTLAGAIIPELVKVFTSTESAHVREVVISSREGGASLNVLSG
                     LVAGNFSAYWLGMAIVFLMGTAWWVSTFGLSAIMAASPVFAFGLVAFGFLGMGPVTIA
                     VDSYGPVTDNAQSVYELSVIETLPNIKQEIKKDHGFDVNFETAKHLLEENDGAGNTFK
                     ATAKPVLIGTAVVGATTMIFSIIVLLTHGLTQNLDRLSLMHPPFLLGLITGGAVIYWF
                     TGASMQAVTTGAYRAVEFIKANIKLEGVERASVEDSKKVVEICTQYAQKGMFNIFLTI
                     FFSTLAFAFFEPYFFIGYLISIALFGLYQAIFMANAGGAWDNAKKVVETELKAKGTPL
                     HDATVVGDTVGDPFKDTSSVAMNPVIKFTTLFGLLAVELAVDMTSQSGLTHILSAAFF
                     AVSLVFVWRSFYGMRIQTGSAA"
     gene            2800..3291
                     /locus_tag="VFD06_00740"
     CDS             2800..3291
                     /locus_tag="VFD06_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02091.1"
                     /translation="MRATLLRAGVVAGILALCGPGIVLADDQAQPADSGKPDSKAGKR
                     KSFGKGENWETFAKIFDANSDGKVSKEEFLAKRPGFDRVDSNHDGSVTQEEVKALPAV
                     QKKGGTGGGFVAHFDGDADGKVSTAEYDAKRSAFFDKVDKNKDGAIEQSELKAAPPGE
                     AGL"
     gene            complement(3328..3804)
                     /locus_tag="VFD06_00745"
     CDS             complement(3328..3804)
                     /locus_tag="VFD06_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02092.1"
                     /translation="MKTRLSLIPALTLGVLVAVLAAGRAAPAHAGPYTDQLSQCLMDS
                     TTEQDKSNLVRWVFASTAQHPDVAAYATVPPEERAALSKSVSTLFERLLTQDCRTQFR
                     EARKNDGEETVSTSFGILVQTALRGLIENPSVSSALVGVDDHLDKQKIAGAMADAR"
     gene            3977..4390
                     /locus_tag="VFD06_00750"
     CDS             3977..4390
                     /locus_tag="VFD06_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02093.1"
                     /translation="MGLFDSLRGALDGAKVAKAFAAFEARRQRPAIHSDADGIGVTLD
                     PDHPDFIEPLLSWLQVRRVTALKRDLAARDLICLQFEAASHQVYEVHEEMVGWHDLIA
                     SLPRHLPGCLTEGEMLEKVMNPPFAANETVIFRRD"
     gene            4403..>5132
                     /locus_tag="VFD06_00755"
     CDS             4403..>5132
                     /locus_tag="VFD06_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HZN02094.1"
                     /translation="MRSSARRRDGSSGPADPESLRLAEDAARTKNWKRWGPYLSERQW
                     GTVREDYSADGNCWDYFPHEQARSRAYRWGEDGLLGITDRECRLCFAFALWNGRDPIL
                     KERLFGLGGPEGNHGEDVKEAYFYLDSTPTHSYMKALYKYPQREFPYAALVRENRARG
                     RNEGEFEIADTGAFEGGAYFDVVAEYGKPSPDDILIRLTVSNRGPEPAPIHVLPTLWF
                     RNTWSWGRSGEGYWEKPSLAADAPD"
BASE COUNT          815 a         1746 c         1698 g          873 t
ORIGIN      
        1 tccgcccgcg gaggagcccc ccgccgaaga gccgcctctg gcgcagcagc cgcccccgga
       61 agaggagcct cctcccccgc tggacgaccc gcccccctct cgccgtgacc ccccggcccc
      121 ggatcagccc ccggtgatcg aggatccgcc ccccaccatt gacgcggcgc ccgccgcttc
      181 tctagactga cgcccccgcc gggagacccc cggccatccc agagtccaga atcgccccgc
      241 ccgccgggga gtaccgagtt cgagtggggc gcggattgtt cgccccgaga ggagtcgaga
      301 tgagcctcgt gcgtaccatc ctgcgccgcc cggagggcat gttcctcccg gcggtgctcg
      361 cgttgctgct cctgtccctc gcccccggcg cggcgctcgc gcaggagggg gacgaagtcg
      421 cggcccatcg cggcggcgag gcgtcgctcg tcctgccgga cgtcggacag gcggagttcc
      481 tcggcatgaa cggccggtcg ctcctgctcg gcggactgct cgtctgcgtg ctcgggctcg
      541 ggttcgggct gatgatgctg gcgcaggtga aggcggcccc cgtgcaccgc tccatggcgg
      601 agatctccga gctcatctat gagacctgca agacctacct catcacgcag gggaagttca
      661 tcctgatcct cgaggtcttc atcgggatca ttatcctgct gtattacggg gtcctgcagc
      721 acttcgcggc gatgaaggtc gccatcatcc tgatcttcag cctcatcggc atcggcgggt
      781 cgtacggcgt tgcctggttc ggcatccgca tgaacacctt cgcgaactcc cgcaccgcgt
      841 tcgcgagcct gcgggggagg ccgttcccgc tctattcgat cccgctcaag gccggcatga
      901 gcatcgggat gctcctgatc tcggtcgagc tgttcatgat gctgtgcatc ctgctgttca
      961 tcccggggga ctacgccggc ccgtgcttca tcggcttcgc catcggcgag tcgctcggcg
     1021 ccgcggcgct gcgcatcgcc ggcggcatct tcaccaagat cgccgacatc ggctccgatc
     1081 tgatgaagat cgtcttcaag atcaaggagg acgacgcccg caaccccggc gtcatcgccg
     1141 actgcaccgg ggacaacgcc ggcgacagca tcgggccctc ggccgacggc ttcgagacct
     1201 acggcgtcac cggcgtcgcg ctcatctcct tcatcctggt ggccgtcccg gaggcgctca
     1261 cgcaggtgca gctgctcgtc tggatcttca tgatgcgcat cctgatgatc atcgcctcgg
     1321 ggctgtcgta cgtcctcaac gaggcgtggg caaggagcgc ctacggcaac gccacgaaga
     1381 tgaacttcga gaagccgctg acgacgctcg tgtggctgac gtcggtggtc tcgatggcca
     1441 tgaccttcat cgcgtcctac ctgctgatcc cggatctcgg cgacgggacg ctgtggtgga
     1501 agctgtcgct ggtcatcacc tgcggcaccc tcgccggcgc catcatcccg gagctggtga
     1561 aggtgttcac ctcgacagaa tccgcccacg tgcgcgaggt ggtcatctcc tcccgcgaag
     1621 ggggcgcctc gctcaacgtc ctctcggggc tggtggcggg gaacttctcc gcctactggc
     1681 tcggcatggc catcgtcttc ctgatgggga cggcgtggtg ggtctcgacc ttcggcctgt
     1741 cggccatcat ggccgcctca cccgtgttcg ccttcgggct cgtcgccttc ggcttcctcg
     1801 gcatggggcc ggtgaccatc gcggtcgatt cctacgggcc ggtcaccgac aacgcccagt
     1861 cggtctacga gctgtcggtc atcgagacgc tcccgaacat caagcaggag atcaagaagg
     1921 atcacggctt cgacgtgaac ttcgagaccg ccaagcacct gctggaggag aacgacggcg
     1981 ccggcaacac cttcaaggcg accgccaagc cggtgctcat cggcaccgcg gtcgtcggcg
     2041 cgaccacgat gatcttctcg atcatcgtgc tcctgacgca cgggctgacg cagaacctcg
     2101 accgcctgtc tctgatgcac ccgccgttcc tgctcgggct catcaccggc ggcgcggtca
     2161 tctactggtt caccggcgcg tcgatgcagg cggtgacgac cggcgcctac cgcgccgtcg
     2221 agttcatcaa ggcgaacatc aagctcgagg gggtggagag ggcctcggtc gaggacagca
     2281 agaaggtcgt cgagatctgc acgcagtacg cgcagaaggg gatgttcaac atcttcctga
     2341 ccattttctt ctcgacgctc gcgttcgcct tcttcgagcc gtacttcttc atcggctacc
     2401 tgatctccat cgcgctgttc ggcctctacc aggcgatctt catggccaac gccggcggtg
     2461 cgtgggacaa cgccaagaag gtggtcgaga ccgagttgaa ggcgaagggg acgccgctgc
     2521 acgacgccac cgtcgtcggc gacacggtcg gggacccgtt caaggacacg tcgtcggtcg
     2581 ccatgaaccc ggtgatcaag ttcaccacgc tgttcggcct cctggcggtc gagctggcgg
     2641 tggacatgac gtcgcaatcg ggtttgaccc acatcctgtc ggccgccttt ttcgcggtct
     2701 cgcttgtctt cgtgtggcgg tcgttctacg gtatgcgcat ccagacgggg tcggccgcct
     2761 gacggcggcg cccgcggacc gacaacgagg gaggaatcga tgagagcgac gttgctgcgg
     2821 gcaggggtgg tggccgggat cctggccctg tgcgggcccg ggatcgtcct tgcggacgat
     2881 caagcccagc cggccgactc cggcaagccg gactccaaag cggggaagag aaaatcgttc
     2941 ggcaagggcg agaactggga gaccttcgcg aagatcttcg acgccaacag cgacggcaag
     3001 gtctcgaagg aggagttcct cgccaagcgc cccggcttcg accgggtcga ctccaaccac
     3061 gacggctccg tgacccagga ggaggtcaag gcgctgccgg cggtccagaa gaagggcggg
     3121 accgggggcg ggttcgtggc ccacttcgac ggtgatgccg acggcaaggt cagcaccgcc
     3181 gagtacgacg ccaagcgctc cgccttcttc gacaaggtgg acaagaacaa ggacggcgcc
     3241 atcgagcagt ccgagctcaa ggccgcccct ccgggcgagg cgggtctcta gtcccggccg
     3301 attcgttctc gtctcgaaat cagacggcta ccgcgcatcg gccatcgcgc cggcgatttt
     3361 ctgcttgtcc agatggtcat cgacgccgac gagcgcgctc gaaacgctcg ggttctcgat
     3421 cagcccgcgc aaggcggtct ggacgaggat gccgaagctc gtcgaaacgg tctcctctcc
     3481 gtcgttcttg cgggcctcgc ggaactgagt gcggcagtcc tgggtcagca ggcgctcgaa
     3541 cagcgtcgag accgacttcg acagggccgc tctctcctcc ggcgggaccg tcgcgtacgc
     3601 ggccacgtcc gggtgctggg ccgtggacgc gaagacccag cgcaccaggt tcgatttgtc
     3661 ctgctccgtc gtcgaatcca tcaggcattg cgacagctgg tcggtgtacg gcccggcgtg
     3721 cgccggggcg gcacgtcccg ccgccagcac cgcgacgaga acgccgagcg tgagcgcggg
     3781 gatcaatgac aggcgtgtct tcacgaaggc ctccttccag tacagcggcg accatggtgg
     3841 ccttcgccct gcggtcggac catccgtggt cttccgccgg aggtgggtca ggctttgccc
     3901 gcggcggggg tccggcggcg gacgcacgga gctcgaaatc gaggaattgg gtcattcctg
     3961 atacgctttc gtccccatgg gtctgttcga ttcgctgcgc ggcgccctcg acggcgcgaa
     4021 ggtggcgaag gcgttcgcgg ccttcgaggc acgccgccag cgtccggcga tccatagcga
     4081 cgccgacggg atcggcgtga cgctcgaccc ggatcatccc gacttcatcg agccgctcct
     4141 ctcctggctt caggtgaggc gggtcaccgc gctgaagcgg gatctcgccg cgcgcgatct
     4201 catctgcctg cagttcgagg cggcgagcca ccaggtctac gaagtgcacg aggagatggt
     4261 gggctggcac gatctgatcg ccagcctgcc gcggcacctt ccgggctgcc tgaccgaagg
     4321 ggagatgctc gagaaggtga tgaatccccc gttcgccgcg aacgagacgg tgatcttccg
     4381 ccgcgactag gagcctgtcc ggatgcgttc gtcggcacgg cggcgggacg gctcgtccgg
     4441 accggccgat cccgaatccc tgcggctcgc ggaggacgcg gcgcgaacga agaactggaa
     4501 gcgctggggc ccctacctca gcgagcgcca gtggggcacg gtccgggagg actactcggc
     4561 ggacggcaac tgctgggact acttcccgca cgagcaggcc aggagccgcg cctaccgctg
     4621 gggggaggac gggcttctcg gcatcaccga ccgggagtgt cgcctgtgct tcgccttcgc
     4681 cctgtggaac ggccgcgatc cgatcctcaa ggagcggctc ttcggcctcg gcggtcccga
     4741 ggggaaccac ggggaggacg tcaaggaggc gtacttctac ctcgactcga cgccgacgca
     4801 ctcctacatg aaggcgctct acaagtaccc gcagcgcgag ttcccgtacg ccgcgctcgt
     4861 gcgcgagaac cgcgcccgcg gccggaacga gggggagttc gagatcgccg acaccggcgc
     4921 cttcgagggc ggagcgtatt tcgatgtcgt cgccgagtac ggcaagcctt cccccgacga
     4981 catcctcatc cggctcacgg tgtccaaccg cgggcccgag ccggccccga tccacgtcct
     5041 gccgaccctg tggttccgca acacctggtc gtggggccgg agcggcgaag ggtactggga
     5101 gaagccatcg ctcgcggcgg acgctccgga cc
//
LOCUS       DASRIQ010000028         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1053934, whole genome shotgun sequence.
ACCESSION   DASRIQ010000028 DASRIQ010000000
VERSION     DASRIQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053934"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..2358)
                     /gene="aroF"
                     /locus_tag="VFD06_00760"
     CDS             complement(202..2358)
                     /gene="aroF"
                     /locus_tag="VFD06_00760"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZN02095.1"
                     /translation="MEAGCDPKDDAGDPVARHRGTIDRLDRDLIRLLAERLQAARAIG
                     DHRSRNKDAALRDDDRERRLFEAWAREAEDRGISAYFAGRVLREVLNWSRRDQERFLG
                     GAAPGSAAARSARIGYQGVPACYSDLAIGKIFATREVARLERVGFRGFAAVIDAMESG
                     EIDYALLPIENTIAGSINEVYDLLGHRPVTIVGEEVWEVEHCLLGLPGARLEELRVVR
                     SHPVALQQCAKFLDRLVGVTSESFHDTAGAAQAVAREGVPSVAAIASEDAAREYGLVV
                     LKRDISDQPVNLTRFLLIGREAEPFDARRPAKTSLVFSVNHRRGALLECLKCFDAQGI
                     NLTKLESRPQANAPWEYQFYLDFEGHVTEPGAVAALQEIRAHTNHLKVLGSYPRRIES
                     DRERQALDPAHAGGRLEASAVAGSVARETPAGAAGAPASEAAATGGVAASEPPANPAL
                     RLTGLKSGAPRSRVDVSGVEFGGVRFVLAIGPCAVESRQQMMESAELVKRHGAAIMRG
                     GAFKPRTSPYAFQGLGFAGLDLLVEAGRAYELPVVTEVLHPEDVERIGASADMLQVGA
                     RNMQNFALLKEVGRARRPVLLKRGLSATIEELLLAAEYVMSGGNHRVVLCERGIRTFE
                     TATRNTLDISAVPVLKERTHLPVIVDPSHAAGRRSLVIPLALAAAAAGADGLIIEAHP
                     HPDDARCDKEQALTDEDLATLVAALRPILAAQGRHL"
     gene            complement(2469..3221)
                     /locus_tag="VFD06_00765"
     CDS             complement(2469..3221)
                     /locus_tag="VFD06_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZN02096.1"
                     /translation="MKIATWNVNGIRARCNEVIEWAEREKPDVICLQEIKATADQVPL
                     PLCNLPGYWCYWHGNKGYSGVGLHVRKDFAPERPEFSHPPFDHESRVVTAKIGALTVA
                     SIYVPNGNKDFAAKLRFLGAMEEFAGAFAANAAPLVLCGDLNVAIEEIDVHPRERRRN
                     IVGQRPEERALMRRILDRGLTDLGRALDPGNDRMFSWWAPWRSLRERNIGWRLDYVLA
                     SADLAGRALACPSYRDVGTSDHAPVVALFRPA"
     gene            3742..>4067
                     /locus_tag="VFD06_00770"
     CDS             3742..>4067
                     /locus_tag="VFD06_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02097.1"
                     /translation="MPIFLAALLSVAGAEAVFAGQCTQVSLPTGWYCEDVGNTPAAAY
                     SMATATGSSPQDVFTVRGGGADNGDFYRGHEGSLHEEYPDNAGLIAGNFSDSFVFAYR
                     PWSGDF"
BASE COUNT          636 a         1423 c         1384 g          624 t
ORIGIN      
        1 gctccccagg cacgagagca gcagcgcgac ccacgccatc ctgtgcagca tcgagccatc
       61 tccgagaaat ggcagacgcg gacgaatctc agggagcgaa cgtgccggga ggatacgccc
      121 ggtcgttcac gtgcgcaacc tgtttccggg gcaccacccg gtcgaaacgg agtcccgcgc
      181 ggtcccgagg ccgcgaccgg ctcagagatg gcgtccctgc gccgcgagga tcggccgtag
      241 cgcggcgacg agggtcgcga ggtcctcgtc ggtcagggcc tgctccttgt cgcaccgggc
      301 atcgtccggg tggggatgcg cctcgatgat gagcccgtcc gctccggcgg ccgccgccgc
      361 gagcgccagc ggaatcacca gcgaccgccg cccggccgcg tgcgagggat cgacgatcac
      421 cggcaggtgc gtgcgctcct tgaggactgg cacggcgctg atgtcgaggg tgttgcgcgt
      481 cgccgtctcg aaggtccgga tgccgcgctc gcagagcacg acgcggtggt tgcccccgga
      541 catgacgtac tcggcggcga ggagtagctc ctcgatggtc gcgctgagcc cgcgcttcag
      601 cagcaccggc cggcgggccc gccccacctc cttcaggagc gcgaagttct gcatgttgcg
      661 cgccccgacc tgcagcatgt cggcgctcgc gccgatccgc tcgacgtcct cgggatgcag
      721 gacctcggtg accaccggga gctcgtaggc acgtcccgct tcgaccagca ggtcgaggcc
      781 cgcgaacccc agtccctgga acgcgtaggg cgaagtgcgc ggcttgaacg cgccgccgcg
      841 catgatggcg gcgccgtggc gcttcaccag ctcggccgac tccatcatct gctgccggct
      901 ctcgacggcg cagggtccga tggcgaggac gaaccgcacg ccgccgaact ccacgccgct
      961 cacgtcgaca cgcgaacgcg gcgcgccgct cttgaggccg gtcaggcgga gggcggggtt
     1021 ggcgggcggt tcggacgccg cgacgccgcc agtggccgcc gcctcgctcg ccggcgctcc
     1081 tgccgcgccc gccggcgtct cgcgtgcgac cgaaccggcg accgccgatg cctcgagccg
     1141 tccgcccgcg tgcgccggat cgagagcctg ccgctcccga tcgctctcga tgcggcgcgg
     1201 ataactgccg agcaccttga gatggttggt gtgagcgcgg atctcctgga gcgccgccac
     1261 ggcacccggc tcggtcacgt gcccttcgaa gtccaggtag aactggtatt cccagggggc
     1321 gttcgcctgc gggcggctct ccagcttggt gaggttgatc ccctgagcgt cgaagcactt
     1381 cagacattcg aggagcgcgc cgcgccggtg gttcaccgag aacaccagcg aggttttcgc
     1441 cggccggcgc gcgtcgaacg gctccgcctc ccggccgatg agcaggaagc gggtcaggtt
     1501 cacgggctga tcggagatgt cccgcttcag tacgaccagc ccgtattcac gcgccgcgtc
     1561 ttcggacgcg atcgccgcga cggacggaac cccctcgcgc gccacggcct gcgcggcgcc
     1621 ggcggtgtcg tggaagcttt ccgaggtgac cccgaccagc cggtcgagga acttggcgca
     1681 ctgctgcagg gcgaccgggt ggctgcgcac gacgcgcagc tcctcgaggc gcgcgccggg
     1741 caggccgagc aggcagtgct ccacctccca cacctcctcg cccacgatgg tcacgggacg
     1801 gtggccgagc aggtcgtaga cctcgttgat gcttcccgcg atggtgttct cgatcggcag
     1861 gagggcgtag tcgatctcac ccgactccat cgcgtcgatc accgccgcga acccgcggaa
     1921 ccccacgcgc tccaggcgcg cgacctcgcg cgtcgcgaag atcttgccga tcgcgagatc
     1981 gctgtagcag gcgggcaccc cctggtaccc gatgcgcgcc gaccgggccg cggctgaacc
     2041 cggcgcggcg ccgccgagga agcgctcctg atcgcggcgc gaccagttga ggacctcgcg
     2101 cagcacgcgg ccggcgaagt aggccgaaat cccccggtcc tcggcctcgc gcgcccaggc
     2161 ctcgaagagc cggcgctcgc ggtcgtcgtc gcgcagggcg gcgtccttgt tccggctccg
     2221 gtgatcgccg atggcgcgcg ccgcctgcag ccgttccgcc aggagacgga tcagatcccg
     2281 atcgaggcgg tcgatcgtcc cgcggtgccg cgccaccgga tcgccggcgt catccttcgg
     2341 gtcgcagcct gcttccaccg gatcgccggt ttcatcctct gaatcgcggg gggaatccat
     2401 tgaactcatc atatacgggg agagccggcg cctccttcgg caggccccgc ggcggcgagg
     2461 agcagccatc aggcaggccg gaacaaggcg acgaccgggg cgtggtcgct cgtgccgacg
     2521 tcccggtagg agggacacgc gagggcacgg cccgcgagat cggccgaggc caggacgtag
     2581 tcgagccgcc atccgatgtt ccgctcgcgc aggctgcgcc agggggccca ccacgagaac
     2641 atccggtcgt tccccggatc gagcgcgcgc ccgaggtcgg tcagaccgcg atcgaggatg
     2701 cgccgcatca gcgcccgctc ctccggccgc tggccgacga tgttgcgccg ccgctcccgc
     2761 gggtgcacgt cgatctcctc gatggcgacg ttgagatcgc cgcacaggac gagcggcgcc
     2821 gcgttggccg cgaaggcccc ggcgaactcc tccatcgccc cgaggaaacg cagcttcgca
     2881 gcgaagtcct tgttgccatt cgggacatag atcgaagcga cggtgagcgc cccgatcttc
     2941 gcggtgacga cccggctctc gtgatcgaac ggaggatggg agaactccgg ccgctccggc
     3001 gcaaagtcct tgcgcacgtg caggcccacg ccggaatagc ccttgttgcc gtgccagtag
     3061 caccagtaac cgggcaggtt gcacagcggc agcggaacct gatcggcggt cgctttgatc
     3121 tcctgcaggc agatcacgtc cggcttctcg cgctccgccc actcgatcac ttcattgcag
     3181 cgcgcgcgga tgccgttcac gttccaggtc gcgatcttca tcggagcaag ggtagtggcg
     3241 gagtcggtgc gagtcgaatt gcaatacgcg ccagcatcac cacgctgctg aacacaccgt
     3301 tgacgacctc ccacacgcgc tggcagtcga gcgttccggc ggcttctctg gcttgggctc
     3361 ccgatcccgg gcgctggcgg agaagagcgc cggctatcgg agcgaaccga cggcgccgga
     3421 cctcggtgcc ggcgacgtgg tacgcgccct gtcctcagga gcaacgctcg tcgcgtacgc
     3481 tcggtattaa cccgcatggg ttcgcgtccg ccccccaggg atcggcctat gtggtcttcg
     3541 tcctgacgac acccacgcgt gattttccag ctgaaccagc aacctttagc ccagtgacat
     3601 cgaccaacag tccgagggcg gaaatgtgaa ccaggtgtgt gtccccaaga cgatgccacg
     3661 gatgactcac catagcgtta tctcatcgag catggaggag cgacgaccat gacaatcgaa
     3721 tcttcgattc cgcgaggcgc ggtgccgatc ttcctggcag cactattgag tgtcgcggga
     3781 gccgaggcgg tcttcgccgg gcaatgcacg caagtttccc tgccgacggg ctggtactgc
     3841 gaagacgtcg gaaacacgcc ggcggcggcg tatagcatgg caaccgcgac cggctcgtcc
     3901 cctcaagacg tgttcacggt gcggggaggg ggtgccgaca acggcgactt ttatcgtggc
     3961 cacgagggaa gtttgcatga ggagtatccc gataacgctg ggctgatcgc cggcaacttc
     4021 tcggactcat tcgtattcgc ctaccgtccg tggtcgggcg atttcga
//
LOCUS       DASRIQ010000029         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_528994, whole genome shotgun sequence.
ACCESSION   DASRIQ010000029 DASRIQ010000000
VERSION     DASRIQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528994"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /gene="trpC"
                     /locus_tag="VFD06_00775"
     CDS             <1..360
                     /gene="trpC"
                     /locus_tag="VFD06_00775"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="involved in tryptophan biosynthesis; amino acid
                     biosynthesis; converts
                     1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate to
                     C(1)-(3-indolyl)-glycerol 3-phosphate and carbon dioxide
                     and water; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HZN02098.1"
                     /translation="LDARLLNTLIGITRSLEMEALVEVHSEEDVARAIDCGARLFGVN
                     NRDLTTMQTSLETSLRLAPLLPPQAAKVSESGIETRADIERLRAAGYDAFLVGGRLMR
                     DADPGAALRSLIEGEAA"
     gene            357..974
                     /locus_tag="VFD06_00780"
     CDS             357..974
                     /locus_tag="VFD06_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZN02099.1"
                     /translation="MRVRVKVCGVTRPEDAAAAVAAGADAIGLNFVEESPRRLTLERA
                     RDVAATIPPFVARVGIFANPDPDAVGRLAEALRLDVAQLHGEETPGEAARVPIPWYKA
                     HRVAAGFDPEVIAAYGRPVALLDAAGPARGGTGRTFDWSAARSGARFARIILAGGLTP
                     ENVGAAIREASPWAVDVNSGVESSPGVKDPALLARFFDAVAAVRP"
     gene            971..2203
                     /gene="trpB"
                     /locus_tag="VFD06_00785"
     CDS             971..2203
                     /gene="trpB"
                     /locus_tag="VFD06_00785"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZN02100.1"
                     /translation="MTTRGAGSEEGRFGPYGGRYVPETLMAPLLELEEAWRQARSDAA
                     FVSRLRAVLGEYAGRPTPLFRADNLARAVGGADGLRLYLKREDLAHTGAHKINNSLGQ
                     ALLAKRMGKTRLIAETGAGQHGVATASAAALLGLECVVYMGEEDMRRQELNVVRMRLL
                     GAEVRPVGAGSRTLKDAISEAMRDWVTNVRDTHYVLGSVLGPHPYPSLVRDFQSVIGH
                     EARQQILAAEGRMPDALVACVGGGSNAIGLFHAFLQDDGVAMFGVEAGGTGILPGQHA
                     ARFAGGSPGVLHGTRSFILQDEDGNVEATHSVSAGLDYAAVGPEHAALRDAGRVTYAH
                     VDDAEALAAFGTLARSEGILPALESSHALAFVAREVGRGALSGVVIVNLSGRGDKDVS
                     EVARLLGAGTAAPPERPR"
     gene            2200..2994
                     /gene="trpA"
                     /locus_tag="VFD06_00790"
     CDS             2200..2994
                     /gene="trpA"
                     /locus_tag="VFD06_00790"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZN02101.1"
                     /translation="MSRIAAAFAAARAEQRAAFIPYLTAGDPDLRATPVLVRAMAAGG
                     ADIVEIGVPFSDPIADGVVNQRAAERGRRAGATLRAILDCCSGLAKEALPPIVLFTYF
                     NPIHRLGVEAFAARAADCGVAGVLVTDLPPEEAGPLRDNLDRRGIDAVGLLAPTSTAE
                     RRRAIAESARGFLYFISRTGVTGARADLPPDLEAHVREARAAAHGLPIAVGFGLSRPE
                     QVRAVRAFADGFVVGSALVRLIEERASDPGLPQALERACRALREAA"
     gene            2991..4073
                     /locus_tag="VFD06_00795"
     CDS             2991..4073
                     /locus_tag="VFD06_00795"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZN02102.1"
                     /translation="MTRRLRILAALLLSGGLVSAAASAGDPTRPSAAAPPRPGAGEPA
                     RPAAHSEGQLLVLNKADDNLMVFDAPSHQLLKTIPVGREPHEVAVTPDGRKAYVANVG
                     DDSLSVVDLKEGRVRRTLKPDGMDQPHGLAITPDGRRLFVTSEGSHRLYLIDVRRDVV
                     ERSITTTQRGSHMVTLAGKGTRAWVANRGSDSLSLYDLPGLKLVRTFKVGPGPEGIAV
                     SPNGRFIVTSLQGAGQVAVVDASRSSVLTRLPAGEAPIRVLFPAASPMAIFSNRGSDD
                     VTFIDVAARHVIATVPVGKRPGGMAVNGRGTRLYVANNDSGTVSIVSIPGREVSGTIQ
                     AGRTPDGLAFVSSEGGGVKQGAGGAR"
     gene            4070..>4888
                     /locus_tag="VFD06_00800"
     CDS             4070..>4888
                     /locus_tag="VFD06_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02103.1"
                     /translation="MKVTNNGSFPWRGTPLGDALERLRGVTGAERPDAAWREARDRVA
                     RQAVAAQAGAGCDLVTDGLARRDEPVAEVVARLEGCEAGPETTSSRPGGGRYRVPIVR
                     GELSWKEPVLVEDYLFAATGSPVPVKVVLTGPFTIAHLAEDHAYGDPMALAMAMATAV
                     NQELRALHGAGAAFIQVDEPGLLERPEAFPIFTRVWEVLGRGVGATLAIHLEGGSIAP
                     ISAGLGRLKRLGCLSVDCVSDPENLAAAAAAPLHEGAVLALGLVAGDAGKNPEDA"
BASE COUNT          649 a         1746 c         1843 g          650 t
ORIGIN      
        1 ctcgatgcgc gcctgctcaa caccctcatc ggcatcaccc ggtcgctcga gatggaggcg
       61 ctcgtcgagg tccattccga ggaggacgtg gcgcgcgcca tcgattgcgg cgcgcgcctc
      121 ttcggggtca acaaccgcga cctgacgacg atgcagacga gcctcgaaac ctccctgcgt
      181 ctcgcgccgc tcctcccgcc gcaggcggcc aaggtgagcg agagcggcat cgagacgcgc
      241 gccgacatcg agcggctgcg cgccgccggg tacgacgcct tcctcgtggg cgggcggctg
      301 atgcgcgacg ccgatcccgg cgcggccctg cgatcgctca tcgaggggga ggcagcgtga
      361 gggtgcgcgt caaggtgtgc ggcgtcacgc gtcccgaaga cgccgccgcg gccgtcgcgg
      421 cgggggcgga cgcgatcggg ctcaatttcg tcgaggagag cccgcgccgc ctgacgctcg
      481 agcgggcccg ggacgtggcg gcgacgattc ccccgttcgt ggcgcgcgtc gggatcttcg
      541 cgaaccccga tccggacgcc gtcggacgtc tggccgaggc cctgcggctg gacgtcgcgc
      601 agctgcacgg cgaggagacc ccgggcgagg ccgcccgcgt gccgatcccc tggtacaagg
      661 cgcaccgggt cgccgccggc ttcgatcccg aagtgatcgc ggcgtacggc cggccggtgg
      721 cccttctgga cgcggcgggg ccagcccgag ggggcaccgg acggaccttc gactggagcg
      781 ccgcgcggag cggcgcccgc ttcgcgcgga tcatcctggc cggagggctg acgcccgaga
      841 acgtcggcgc cgccatccgc gaggcgtccc cctgggcggt ggacgtcaac agcggcgtcg
      901 aatcgtcccc cggcgtcaag gacccggcgc ttcttgcgcg cttcttcgac gcggtggcgg
      961 cggtgcgtcc atgacgacgc gcggggcggg gtccgaggag ggacgcttcg gaccgtacgg
     1021 cggccgctac gttcccgaga ccctgatggc gccgctcctc gagctggagg aggcctggcg
     1081 ccaggcgcgg agcgacgccg ccttcgtctc gcggctgcgg gccgtcctcg gcgaatacgc
     1141 ggggaggccg acgccgctgt tccgggcgga caacctggcg cgcgcggtcg gaggcgcgga
     1201 cggtctgcgc ctctacctca aacgggagga cctggcgcac accggggcgc acaagatcaa
     1261 caacagcctg ggccaggccc tcctcgcgaa gcggatggga aagacgcgtc tgatcgccga
     1321 gaccggggcg ggacagcacg gcgtcgcgac cgcgagcgcc gcggctcttc tcggtctcga
     1381 gtgcgtcgtc tacatggggg aggaggacat gcggcggcag gagctgaacg tcgtccgcat
     1441 gcggctcctc ggggccgagg tgcgccccgt cggcgccggc agccgcaccc tgaaggacgc
     1501 gatcagcgaa gccatgcgcg actgggtgac gaacgtccgt gacacccact acgtcctcgg
     1561 gtcggtcctg ggtccgcatc cctacccgtc gctggtgcgc gacttccagt cggtcatcgg
     1621 gcacgaggcg cggcagcaga tcctcgcggc ggaaggacgc atgccggacg cgctggtcgc
     1681 ctgcgtcggc ggcggcagca acgccatcgg ccttttccac gctttcctgc aggacgacgg
     1741 cgtcgccatg ttcggcgtcg aggcgggagg gaccggaatc ctcccgggac agcacgcggc
     1801 ccgcttcgcc ggaggcagtc ccggagtgct gcacggcacc cgctcgttca tcctccagga
     1861 cgaggacggc aacgtcgagg cgacccactc ggtgtcggcc ggtctcgact acgccgccgt
     1921 cgggccggag cacgcggcgc tccgggacgc ggggcgcgtc acctacgctc acgtggacga
     1981 cgccgaggcg ctggccgcgt tcgggacgct cgcccgaagc gaggggatcc tgccggccct
     2041 ggagtcgtcg cacgcgctgg cgttcgtcgc gcgcgaggtg gggcgcgggg cactctcggg
     2101 cgtcgtcatc gtgaatctct ccggccgcgg ggacaaggac gtgagcgagg tggcgcgcct
     2161 cctgggcgcc gggacagccg cgccgccgga gcgtccgcga tgagccggat cgccgccgcc
     2221 ttcgccgccg cgcgggccga gcagcgcgcc gccttcattc cgtacctgac ggccggcgat
     2281 cccgatctgc gcgccacgcc ggtcctcgtc cgggccatgg cggcgggggg tgcggacatc
     2341 gtcgagatcg gcgttccctt ctccgatccg atcgccgacg gcgtggtcaa ccagcgggcc
     2401 gccgaacgag gccggcgcgc cggggcgacc ttgcgagcga ttctcgactg ctgctccggc
     2461 ctggccaagg aagcgctccc gccgatcgtg ctgttcacct acttcaaccc gatccaccgc
     2521 ctcggcgtcg aggccttcgc ggcgcgggcc gcggactgcg gggtcgcggg tgtgctggtc
     2581 acggatctcc ctcccgagga ggcgggaccg ctgcgcgaca acctggatcg ccgggggatc
     2641 gacgcggtcg ggctcctggc gccgacctcc accgccgagc ggcgccgcgc catcgcggag
     2701 tcggcgcgcg gcttcctcta tttcatctcc cggacgggcg tgaccggggc ccgggccgat
     2761 ctgccgcccg acctcgaggc gcatgtgcgg gaggcgcgcg ctgccgcgca cggtctcccg
     2821 atcgcggtcg gattcgggct gagccggccg gagcaggtcc gcgccgtccg cgccttcgcc
     2881 gacggtttcg tcgtcggcag cgccctcgtc cgactcatcg aggagcgcgc ctccgatccc
     2941 ggcctgccgc aggcgctcga gcgcgcctgc cgcgctctcc gggaggccgc gtgacgcgcc
     3001 gcctccggat cctcgcggcc ttgctcctct cgggcggcct ggtctccgcc gcggcgagcg
     3061 ctggtgaccc gacgcgcccg tcggcggccg cgccgcctcg ccccggggcg ggggagccgg
     3121 cgcggccggc cgcgcactcc gaaggccagc tgctcgtcct caacaaggcg gatgacaacc
     3181 tcatggtgtt cgacgcgccg tcgcaccagc tcctgaagac cattccggtc ggccgcgagc
     3241 cgcacgaggt ggccgtgacc cccgacgggc gcaaggccta cgtggccaac gtcggtgacg
     3301 acagcctgtc ggtcgtggac ctgaaggagg gacgcgtccg ccgcaccctc aagcccgacg
     3361 gcatggacca gcctcacggt ctggccatca cgccggacgg ccgccgcctg ttcgtgacca
     3421 gcgaggggag ccaccgtctc tacctcatcg acgtccgccg cgacgtcgtc gagcgcagca
     3481 tcaccacgac gcagcgcgga tcgcacatgg tgacgctcgc cggcaagggg acccgcgcct
     3541 gggtcgcgaa ccggggctcc gattcgctga gcctctacga cctgcccggg ctgaagctcg
     3601 tcagaacgtt caaggtcggg cccggcccgg aagggatcgc cgtcagtccg aacggccggt
     3661 tcatcgtcac gtcgctccag ggggcggggc aggtggcggt ggtggacgcg tcgcgctcga
     3721 gcgtgctgac acggctgccg gccggcgagg ccccgatccg ggtgctgttc ccggccgcct
     3781 caccgatggc gatcttcagc aaccgcggca gcgacgacgt caccttcatc gacgtggcgg
     3841 cgcggcacgt catcgcgaca gtgccggtcg ggaaacgtcc gggtggaatg gcggtgaacg
     3901 ggcgcggcac caggctgtac gtcgccaaca acgactcggg gaccgtgtcg atcgtctcga
     3961 tccccgggcg cgaggtgagc gggacgatcc aggcgggccg gacgcccgat ggtctggcgt
     4021 tcgtgtcgtc ggaaggaggg ggcgtgaagc agggcgcagg aggggcgcga tgaaggtcac
     4081 caacaacggc tcgttcccgt ggcggggcac cccgctcgga gacgcgctcg agcggctgcg
     4141 cggcgtgacc ggcgccgagc ggccggacgc cgcgtggcgc gaggcgcgcg atcgggtggc
     4201 gcgtcaggcg gtcgcggccc aggccggcgc cggctgcgac ctcgtcaccg acgggctggc
     4261 gcgccgggac gaaccggtgg ccgaggtggt ggcgcgcctc gaaggctgcg aagccggccc
     4321 ggagacaacg tcctccaggc cgggaggcgg ccgatatcga gtcccgatcg tgcgcggcga
     4381 gctgtcctgg aaggaaccgg tcctggtcga ggactacctc ttcgccgcga ccggttcgcc
     4441 cgtcccggtg aaggtcgtgc tcacgggacc cttcacgatc gcgcacctgg cggaggatca
     4501 cgcttacggc gatccgatgg cgctcgcgat ggcgatggcg acggccgtga accaggagct
     4561 gcgggcgctc cacggagccg gagcggcgtt catccaggtg gacgagccgg ggctcctgga
     4621 gcggccggag gcgttcccga tcttcacgcg cgtctgggag gtcctcggcc gcggcgtcgg
     4681 ggcgacgctg gcgatccatc tcgaaggagg gagtatcgcg ccgatctccg ccgggctcgg
     4741 gcggctgaaa cgcctcgggt gcctgagcgt cgactgcgtc agcgatccgg agaacctcgc
     4801 ggcggcggcc gccgcaccgc tgcacgaagg ggcggtgctg gcgctcggcc tcgtcgcggg
     4861 agatgccgga aagaacccag aggacgca
//
LOCUS       DASRIQ010000030         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_787903, whole genome shotgun sequence.
ACCESSION   DASRIQ010000030 DASRIQ010000000
VERSION     DASRIQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787903"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFD06_00805"
     CDS             complement(<1..207)
                     /locus_tag="VFD06_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02104.1"
                     /translation="MRPFRKVSRLRFGKGLIATRAIPRGALVASFRGARTRGAPTYQS
                     VQIGPQTHVDDPTCLNLLNHSCAPT"
     gene            complement(204..1727)
                     /locus_tag="VFD06_00810"
     CDS             complement(204..1727)
                     /locus_tag="VFD06_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZN02105.1"
                     /translation="MRPSRVTPVRVLAGAAFAILLASPRGAAAETSAAAPQKVTAATP
                     DRPPSEPGPFRSPDLVELTTLDPTLRLDIRYARKDNFTGAAVYSEARAFLQAPAAEAL
                     LRAHRSLRPKGYGLLVFDGYRPWSVTRRFWELTPADKKVFVADPAQGSKHNRGCAVDL
                     TLYDLATGREVAMPSPYDDMTAKAYATYDGGTPEARSRREILREAMEREGFFVHPHEW
                     WHFDYKDWREYPILDVPFSAIKTGTPSGAPRAAGVPWDLSKVRMIDLTWTFDATTLYW
                     PTSPSAFELKQLSRGPTEAGYFYSANAFCAPEHGGTHLDAPIHFAEKGWTTEQIPLER
                     LIAPGVVIDVTRQAAGDVDYRLTREDIERWEEEHGRIPAGSIALLRTGWGRRWPDRAR
                     YFGSAAPKDAVHLHFPGYGEAAARLLVEGRRVAALGIDTPSIDYGPSRDFRVHVVAMS
                     ANVIGLENVAHLEDVPATGALVVALPVKIGGGSGGPLRLAALVPSAGGAPTAGGGSR"
     gene            complement(1834..>2833)
                     /locus_tag="VFD06_00815"
     CDS             complement(1834..>2833)
                     /locus_tag="VFD06_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HZN02106.1"
                     /translation="SERIGFIARLDDDPEAIDYTFYVTAHEVGHMWWAHQVMGANVQG
                     ATLMSETMAQYSALMVMEKEYGRNKMRRFLKYELDAYLGGRGAERVEEMPLYLVENQP
                     YIHYRKGSLIMYALRDQIGEAPLNAALKEYVAAVKFQEPPYTNSPEFLDYVSKAVPPE
                     KMAMLEDLFRQITLYENRATKATWAKREDGKYVVTLQFAAAKYRADGKGKETEQPLDD
                     WVDIGIFGEKGEGTPPEGKVLLLEKRHLAKGEDRIEVVVDALPKKAGIDPFNKLIDRN
                     PENNLTTVAEGGPALPPGSDLEKHPGPVPAPGTANAASDPAPAGEAPPSAEPAASQ"
BASE COUNT          390 a          975 c         1010 g          458 t
ORIGIN      
        1 ggtcggagcg cacgaatggt tgagcaggtt gaggcacgtc gggtcgtcca cgtgcgtctg
       61 cggcccgatc tgcaccgact ggtaggtggg ggcgccgcgc gtcctcgctc cccggaagga
      121 ggcgacgagg gcgccgcgcg ggatcgctcg ggtcgcgatg agacccttgc cgaaccgcag
      181 gcgggacact ttccggaacg gcctcatcgg gaccctcccc cggccgtcgg cgcgccgccg
      241 gcggacggga cgagggcggc gagccgcagg ggcccgcccg atccgccgcc gatcttcacc
      301 ggcaacgcga ccacgagggc ccccgtcgct gggacgtcct cgaggtgcgc gacgttctcc
      361 agtccgatga cgttcgcgct catcgcgacg acgtggacgc ggaagtcccg cgacggtcca
      421 tagtcgatgc tcggcgtgtc gatcccgagc gccgcgaccc ggcgtccctc gacgagcaga
      481 cgcgccgccg cctcgccgta gcctgggaag tgcaggtgca ccgcgtcctt gggcgcggcg
      541 ctcccgaagt atcgggcccg gtcgggccag cgccgccccc agccggtgcg caggagcgcg
      601 atcgatccgg ctgggatgcg gccgtgctcc tcttcccacc gctcgatgtc ttcgcgggtc
      661 agacggtagt cgacgtcgcc ggccgcctgg cgcgtgacgt cgatgacgac ccccggggcg
      721 atcagccgct cgagcgggat ctgctcggtc gtccagccct tctcggcgaa atggatcggc
      781 gcgtcgaggt gggtgccgcc gtgctccggg gcgcagaagg cgttggcgct gtagaaatac
      841 ccggcctcgg tcggaccgcg cgacagttgc ttcagttcga atgcggaagg ggaagtcggc
      901 cagtaaagcg tcgtcgcgtc gaacgtccag gtcaggtcga tcatccgcac cttgctcagg
      961 tcccacggca cgcctgccgc gcgaggcgcg ccggaaggcg tgccggtctt gatggcggag
     1021 aacggcacgt cgagaatcgg atactcgcgc cagtccttgt agtcgaagtg ccaccattcg
     1081 tgcgggtgca cgaagaatcc ctcccgctcc atcgcctcgc gcaggatctc gcgccgcgag
     1141 cgcgcctcag gggtcccgcc gtcataggtg gcgtaggcct tggcggtcat gtcgtcgtac
     1201 gggctcggca tcgccacctc gcgtccggtc gcgagatcgt agagcgtcag atcgaccgcg
     1261 cagccgcggt tgtgcttcga accctgcgcc gggtcggcga cgaacacctt cttgtcggcg
     1321 ggggtcagct cccagaagcg gcgggtgacg ctccacggcc gatagccgtc gaagaccagc
     1381 aggccgtaac ccttcggccg gagcgaccgg tgggcgcgca ggagcgcctc cgccgccggg
     1441 gcttgcagga acgcgcgcgc ctcggagtag acggcggcgc cggtgaagtt gtccttgcgc
     1501 gcgtaacgga tgtcgagccg gagcgtcggg tcgagcgtcg tgagctcgac gagatcgggg
     1561 gaacggaatg gccccggctc ggacggcggc cgatcggggg tcgcggccgt caccttctgg
     1621 ggagccgcgg ccgaggtctc cgcggcggct ccgcgaggtg aagccaggag gatcgcgaat
     1681 gcggcgccgg cgaggacgcg gaccggcgtg acacgggatg ggcgcatggt cacctccgcc
     1741 ggagattcta acgaaggtgc gcggcggcga ccgctccggt gcctgacgga ccgctcccgg
     1801 gtcctgcgga gtcgcgtgaa gccaggaccg cggctactgc gacgcggccg gctcggcgga
     1861 tggcggcgcc tcgccggcgg gggccggatc cgacgcggcg ttcgcggtcc ccggcgccgg
     1921 caccggaccc gggtgcttct cgaggtccga tccgggcggc agcgccggcc cgccctcggc
     1981 gaccgtcgtg aggttgttct ccgggttgcg atcgatgagc ttgttgaacg gatcgatccc
     2041 cgccttcttc ggcagcgcgt ccaccaccac ctcgatgcga tcctccccct tggcgaggtg
     2101 gcgcttctcg agcagcaaga ccttcccttc cggcggggtg ccctccccct tctcgccgaa
     2161 gatgccgatg tccacccagt cgtcgagcgg ctgctccgtc tccttcccct tgccgtcggc
     2221 acggtatttc gccgccgcga actgaagggt cacgacgtac ttgccgtcct cccgcttcgc
     2281 ccaggtcgcc ttcgtcgcgc ggttctcgta gagcgtgatc tggcggaaca ggtcctcgag
     2341 catcgccatc ttctcgggcg gcaccgcctt cgagacgtag tcgaggaact ccggagagtt
     2401 ggtgtacggc ggctcctgga acttgaccgc cgccacgtac tccttgaggg cggcgttgag
     2461 cggcgcctcg ccgatctggt cgcgcagcgc gtacatgatg aggctcccct tgcggtagtg
     2521 gatgtacggc tggttctcga ccaggtagag cggcatctcc tcgacccgct ccgcgccacg
     2581 tccgccgagg taggcatcga gctcgtactt gaggaagcgg cgcatcttgt tccggccgta
     2641 ctccttctcc atcaccatca gggcggagta ctgcgccatg gtctccgaca tcagcgtcgc
     2701 gccctggacg ttcgccccca tgacctggtg cgcccaccac atgtgcccga cctcgtgggc
     2761 ggtgacatag aaggtgtagt cgatcgcctc cgggtcgtcg tcgaggcggg cgatgaagcc
     2821 gatgcgctcg gag
//
LOCUS       DASRIQ010000031         5633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2618839, whole genome shotgun sequence.
ACCESSION   DASRIQ010000031 DASRIQ010000000
VERSION     DASRIQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2618839"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VFD06_00820"
     CDS             <1..1153
                     /locus_tag="VFD06_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02107.1"
                     /translation="APRLALSWDPMGDSKTKVFASWGRFYDKLFLNAVIQEEGPDTIT
                     RYYRKDQDGITGGGVPNNGFGAAISKAPPSSTQIDRGLQTPFTDEWSVGFERELAPEV
                     SVSLRYIRRDARLGLQDRDINHTLRNNPNTGQPIDQFGRLVAGSGDGATLFPSPDALP
                     DLYIQNFFFNQVLRLGNYNTSKFTGVELEVSKRLSRKWQMDMSYSYSRAQGNAEDFLS
                     ALGDDPAAVGYEYGYLDFDQRHIVRFNGTTFLPGDWSLGGVFEWTSGLPYSLVTTSFS
                     LDNFSYAQNRTLFGRVVQTGEGPIFVPENRNSRRNASILNIDLAAAKSFVIGRFNSKL
                     FLAVENLLNTDDLEIDTYEPDNPNRGGALQLDADRRFGRRYGIGFQFEF"
     gene            1239..2636
                     /gene="cysS"
                     /locus_tag="VFD06_00825"
     CDS             1239..2636
                     /gene="cysS"
                     /locus_tag="VFD06_00825"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZN02108.1"
                     /translation="MGLRFHNTLTRSVEPFVPLRAGEVGLYTCGPTVYDYAHIGNFRT
                     YLWEDLLRRHLELRGYKVTQVMNLTDVDDKTIAGALRTGRPLDDYTKTYIDAFFEDLD
                     ALGIQRAALYPRATRHVPEMNAMMERLQRKGHLYESKGSIYFRIDTFPAYGRLSGLDP
                     DSAAAGQARIDADEYDKDNPRDFAVWKMRKGEEPFWESPFGAGRPGWHLECSAMSIKY
                     LGETFDIHTGGVDNIFPHHENEIAQSEAATGQPFVRTWMHAAHLIVDGEKMSKSKGNF
                     HTLRDLMAKGYRPRPIRYLLLSAHYRKQLNFTFEGLSQAEASLCRLDDLADRLGRTPL
                     GEGRSDLSDRAERTRGEMLEGLDSDLNTAAALGPLFDLVRDAHTALDAGTARAADAVA
                     VREALALFERVFGIRLGPDACLDADIEALIERRQQARASRDWAAADAIRRELAERGIV
                     LEDTPQGVRWKRKGA"
     gene            2660..3073
                     /locus_tag="VFD06_00830"
     CDS             2660..3073
                     /locus_tag="VFD06_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZN02109.1"
                     /translation="MRPAATEVREEQERYRAARDLGRRAEDEAARYLAERGMRLVARS
                     FRARGGEIDLVVEDGETLVFVEVKARASLSWGRPSEAVGPVKRARMERAARLFLARRA
                     GRPEPPCRFDVVEVLARPGEPLRIRHLPDAFGIDG"
     gene            3151..3225
                     /locus_tag="VFD06_00835"
     tRNA            3151..3225
                     /locus_tag="VFD06_00835"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3183..3185,aa:Gly,seq:gcc)
     gene            3371..3445
                     /locus_tag="VFD06_00840"
     tRNA            3371..3445
                     /locus_tag="VFD06_00840"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3403..3405,aa:Cys,seq:gca)
     gene            3479..5314
                     /locus_tag="VFD06_00845"
     CDS             3479..5314
                     /locus_tag="VFD06_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZN02110.1"
                     /translation="MTLRTLCDIARHLEAAPPKADQLLFKQDGAWRPIDTPTFLRRVG
                     DLARGLRALGVVPGDRVAILSENRPEWTLADHAILFAGAAGVPIYPTLQPDQIGYILQ
                     DSGSKVLFVSSAALRARVEAVRARVPDLKDVVTFEPPGGRPPGVRPLADVEALGARGR
                     AAGGAAGPLGAGGTERLGEAAPSDLASVIYTSGTTGRPKGVMLSHANFAHNVRASCEA
                     IPFEASDVCLSVLPLSHVYERMVEYCYLWRGATIAYAESIESLAACLQEVRPTIVCAV
                     PRLFEKMYQRILDRSVALPRARRLLFNWGLRIAAGWGAAQRRASGPSQPPGPGRLLRA
                     QHALAARLVYAKVLERFGGRLRFFVSGSAPLSPALAEILSGMGIRIVEGYGMTECAPV
                     IAVNRVEAICIGSVGPPIDGIEVRLASDGEIQVRGDSVMRGYWNDPESTRETLQDGWL
                     LTGDIGHFDPHGCLVITDRKKEVLKTSGGKMVAPQPLENLLRADRFIGQAVVLGDRRH
                     FISALLVPDFEMLRSYALHKGLGVIEPVDLLRHPRVLDLFTRRVAAINETLARYERIR
                     KFRLLDRDFTVEAGEMTPTLKPRRKVIEERYRAVIEDMYAEPPPA"
     gene            complement(5266..>5633)
                     /locus_tag="VFD06_00850"
     CDS             complement(5266..>5633)
                     /locus_tag="VFD06_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN02111.1"
                     /translation="ANAGRVGAHLMKGLRGLQEKHAILGDVRGLGLMVGLEIVRDRAT
                     RAPNPEARHEILVQAFERGLLILPCGASTLRLSPPLVCTEQDADTAVAILDAACTAVA
                     GARPQAGGGSAYMSSITAR"
BASE COUNT          833 a         1986 c         1988 g          826 t
ORIGIN      
        1 ggcgccgcgg ctggccctct cctgggaccc gatgggggat tcgaagacca aggtgttcgc
       61 ctcgtggggc cgcttctacg acaagctgtt tctgaacgcg gtcatccagg aagaggggcc
      121 ggacaccatc acccgctact accggaagga ccaggacggc atcaccggcg gcggagtccc
      181 gaacaacggc ttcggggcgg cgatttccaa ggccccgccg agctccaccc agatcgaccg
      241 cgggctgcag acgccgttca ccgacgagtg gtcggtcggc ttcgagcgcg agctggcccc
      301 cgaggtgtcg gtcagcctgc ggtacatccg gcgcgacgcg cgcctcggcc tgcaggaccg
      361 cgacatcaac cacacgctgc ggaacaaccc gaacaccggc cagcccatcg atcagttcgg
      421 gcggctggtc gccggcagcg gcgacggcgc gaccctcttc ccgagccccg acgcgcttcc
      481 cgacctgtac atccaaaact tcttcttcaa ccaggtcctg cggctcggca actacaacac
      541 ctcgaagttc accggcgtcg agctggaggt cagcaagcgc ctgagccgga agtggcagat
      601 ggacatgtcg tactcgtaca gccgcgccca ggggaacgcc gaggacttcc tgtccgccct
      661 cggggacgac ccggccgcgg tcggctacga gtacggctac ctcgacttcg atcagcgcca
      721 catcgtgcga ttcaacggca cgacgttcct gccgggcgac tggagcctgg ggggtgtctt
      781 cgagtggacc tccggactgc cctactcgct cgtgaccacc agcttctcgc tcgacaactt
      841 cagctacgcg cagaaccgga cgctcttcgg ccgcgtcgtc cagactggcg agggcccgat
      901 cttcgttccg gagaaccgca acagccgccg caatgcgtcg atcctgaaca tcgacctggc
      961 ggcggccaag tcgttcgtca tcgggcggtt caactcgaag ctgttcctgg cggtggagaa
     1021 cctgctcaac acggacgacc tggaaatcga cacgtacgag cccgacaacc cgaaccgcgg
     1081 cggcgcgctg cagctcgacg cggaccgtcg cttcggccgg cgctacggga tcgggttcca
     1141 gttcgagttc tgagggaagc aaccgacgga cgaatcccgg gccccgccgc gcgtgacgcg
     1201 cggcggggcc tttgcctgtc ggccggtgcg ggggaccgtt gggcctgcga ttccacaaca
     1261 cgctgacgcg ttcggtcgag ccgttcgtcc cgctgcgcgc gggcgaggtg gggctgtata
     1321 cctgcggccc cacggtctac gactacgccc acatcgggaa cttccgcacc tacctctggg
     1381 aggacctcct ccggcgccac ctcgagctgc gcggatacaa ggtcacccag gtgatgaacc
     1441 tcaccgacgt cgacgacaag accatcgccg gggcgctcag gaccggacgg ccgctcgacg
     1501 actacacgaa gacctacatc gacgccttct tcgaggatct cgacgcgctg ggcatccagc
     1561 gggccgcgct gtacccgcgg gcgacgcggc acgtgcccga gatgaacgcg atgatggagc
     1621 gcctgcagag gaaggggcac ctttacgaga gcaaggggtc gatctacttc cgcatcgaca
     1681 ccttcccggc gtacgggcgc ctgtccggtc tcgatcccga ctcggccgcc gccggccagg
     1741 cgcgcatcga cgcggacgag tacgacaagg acaacccgcg cgatttcgcc gtctggaaga
     1801 tgcgcaaggg ggaggagccg ttctgggagt ccccgttcgg ggcggggcgc ccgggctggc
     1861 acctcgagtg ctcggcgatg tcgatcaagt acctggggga gaccttcgac atccacacgg
     1921 ggggcgtgga caacatcttc ccgcaccacg agaacgagat cgcccagagc gaggcggcga
     1981 cgggccagcc gttcgtccgc acctggatgc acgccgccca cctcatcgtc gatggcgaga
     2041 agatgtcgaa gtcgaagggg aacttccaca cgctgcggga cctgatggcg aagggatacc
     2101 ggccgcggcc gatccgctac ctcctgctgt cggcgcacta ccgcaagcag ctcaacttca
     2161 cgttcgaggg gctgtcgcag gcggaggcgt cgctctgccg cctcgacgat ctcgccgatc
     2221 gcctcggccg cacgcccctc ggcgaggggc ggtccgatct ctcggaccgg gcggagcgga
     2281 cgcgcgggga gatgctggag ggactcgact ccgacctcaa cacggcggcg gcgctcgggc
     2341 cgctcttcga cctcgtgcgc gacgcccaca ccgccctcga cgccggaacg gcgcgggcgg
     2401 ccgacgccgt ggcggtgcgc gaggcgctcg ccctgttcga gcgggtgttc gggatccgcc
     2461 tggggcccga cgcatgcctc gacgccgaca tcgaggcgct catcgagcgc cgccagcagg
     2521 cgcgcgcatc gcgcgactgg gccgcggccg acgcgatccg cagggagctg gcggagcgcg
     2581 gcatcgtcct cgaggacacc ccgcagggcg tgcgctggaa gcgcaaggga gcgtaatgcc
     2641 ggccggagcg gcgtgggcga tgaggccggc cgcgacggag gtccgcgagg agcaggagcg
     2701 ctaccgcgcc gcccgcgacc tcggccggcg tgccgaagac gaggcggccc ggtacctcgc
     2761 ggagcgcggg atgcgcctcg tggcgcgctc gttccgggcg cgcggcgggg agatcgatct
     2821 cgtcgtcgag gacggggaga cgctggtctt cgtcgaggtg aaggcccgcg ccagtctgtc
     2881 gtggggccgg ccgtcggagg cggtggggcc ggtcaagcgc gcccggatgg agcgtgccgc
     2941 gcgcctgttc ctggcgcgcc gcgccggccg tcccgagccg ccgtgtcgct tcgacgtcgt
     3001 cgaagtgctc gcgcgcccgg gggagccgct ccggattcgc catctcccgg acgccttcgg
     3061 aatcgacggg tgacgcggca gggcgcgttc cgccttgaca cggcgggggg ggcgttcgta
     3121 tagttttttg cccatcatgg agacgaatga gcgggaataa ctcagtggta gagtgcaacc
     3181 ttgccaaggt tgaagtcgcg ggttcgactc ccgtttcccg ctccaatcct cagcgccggc
     3241 ccttcggggc cggcgttttt ttgtcggcgc gcgcgcatcg cccgcaccca ggcgcgggca
     3301 cgtgccgttg actccgccgg cgccccccgc tatagtccca acatcctgcg aacgtgagtc
     3361 ttacggtccg ggcggcgtag ccaagcggta aggcagaggt ctgcaaaacc tcgatgcatc
     3421 ggttcgattc cgatcgccgc ctccaccgtc agcccgcgtc atcggccggc gggatccggt
     3481 gacgctgcgc accctctgcg acatcgcgcg ccacctcgaa gccgccccgc ccaaggccga
     3541 ccagctcctc ttcaagcagg atggggcctg gcgccccatc gacacgccga ccttcctgcg
     3601 ccgcgtcggc gatctggcgc gcggcctgcg cgcgctcggc gtggtgccgg gggaccgcgt
     3661 cgcgatcctc tcggagaacc gtccggaatg gaccctcgcc gatcacgcca tcctgttcgc
     3721 cggcgcggcg ggcgtgccga tctacccgac cctgcagccg gaccagatcg gctacatcct
     3781 ccaggactcc ggctccaagg tgctgttcgt ctcctccgcg gcgctgcgcg cccgggtcga
     3841 agcggtccgc gcgcgggtgc cggacctcaa ggatgtcgtg acgttcgagc cgcccggagg
     3901 ccggccgccg ggcgtgcggc cgctcgccga cgtcgaagcg ctcggagcgc gcggccgcgc
     3961 ggcgggaggc gccgccggac cgctgggggc gggcgggacg gagcggctcg gcgaggcggc
     4021 cccctccgat ctcgccagcg tcatctacac ctccggcacg accggacgtc ccaagggggt
     4081 gatgctgtcg cacgccaact tcgcccacaa cgtccgcgcg tcgtgcgagg cgatcccgtt
     4141 cgaggcctcg gacgtgtgcc tctcggtcct gccgttgtcg cacgtctacg agcggatggt
     4201 ggagtactgc tacctctggc gcggcgccac catcgcctac gccgagtcga tcgagtcgct
     4261 ggcggcctgc ctgcaggagg tgcggccgac gatcgtctgc gcggtgccgc gcctcttcga
     4321 gaagatgtac cagcggatcc tcgatcgctc cgtcgcgctg ccgcgggcgc ggcgcctcct
     4381 cttcaactgg gggctgcgga tcgcggccgg ctggggggcg gcgcagcgcc gcgcctcggg
     4441 accgtcgcaa ccgcccggcc cgggccggct cctgcgcgcc cagcacgcgc tcgccgcgcg
     4501 cctggtgtac gcgaaggtcc tggagcgttt cggagggcgg ctgcgcttct tcgtgtcggg
     4561 gagcgcgccg ctctcgccgg cgctggcgga gatcctcagc ggcatgggga tccggatcgt
     4621 cgaggggtac ggcatgaccg agtgcgcccc ggtgatcgcg gtcaaccggg tggaggcgat
     4681 ctgcatcggc tcggtcggcc cgccgatcga cgggatcgag gtgcggctcg cctccgacgg
     4741 ggagatccag gtgcgcggcg attcggtgat gcgcggctac tggaacgatc ccgagtcgac
     4801 gcgcgagacg ttgcaggacg gctggctcct gacgggcgac atcgggcact tcgatccgca
     4861 cgggtgcctg gtgatcaccg accggaagaa ggaggtcctc aagacctcgg gcgggaagat
     4921 ggtggcgccg cagccgctcg aaaatctcct gcgcgccgat cgcttcatcg gccaggcggt
     4981 ggtgctcggc gaccgacgcc acttcatctc cgcgctgctg gtccccgatt tcgagatgct
     5041 ccgctcgtac gccctgcaca aggggctcgg ggtgatcgag ccggtcgacc tgctgcgcca
     5101 cccgcgcgtg ctggacctgt tcacgcgccg ggtcgctgcg atcaacgaga ccctggcgcg
     5161 ctacgagcgg atccggaagt tccgcctgct cgatcgcgac ttcacggtcg aggcggggga
     5221 gatgaccccg accctcaagc cgcggcggaa ggtgatcgag gagcgctacc gggcggtgat
     5281 cgaggacatg tacgcggagc cgccgccggc ctgaggccgg gccccggcca cggcggtgca
     5341 ggcggcgtcg aggatcgcca ccgcggtgtc ggcgtcctgc tccgtgcaga cgagcggcgg
     5401 cgagagccgc agcgtcgagg cgccgcacgg caggatcagg agtccccgct cgaacgcctg
     5461 gacgaggatc tcgtggcgcg cctccgggtt cggcgcgcgc gtcgcccggt cccggacgat
     5521 ctcgagtccg accatcagcc ccaggccgcg cacgtcgccg aggatcgcgt gcttctcctg
     5581 cagcccgcgc aggcccttca tgagatgcgc gccgacccgg ccggcgttgg cga
//
LOCUS       DASRIQ010000032         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2619465, whole genome shotgun sequence.
ACCESSION   DASRIQ010000032 DASRIQ010000000
VERSION     DASRIQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619465"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VFD06_00855"
     CDS             <1..1084
                     /locus_tag="VFD06_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02112.1"
                     /translation="YLLYGRFDNLFAIPWQPTVTDLSGAVPITLPEHPRLENEGGAAY
                     DAAPNGTLVYLPGGPARYGQRVVWVDRDGRTDPVPIPERDLGSVALSPDGRQAAVQVT
                     EGTIGIWLYDFERKTFTPFVTGPGSSQAPLWTPDGTRLVYRGTRHGTRDIYIKPADGT
                     GAETRLTSGLGRSLTATSMTPDGRWLIFSGIGGASGGENMIWRVPLDGAGKPEPMLAP
                     ADRLQNGQISPDGRFMAYSSGISGRPEIYVMPFPGPGPSRQISIGGGYEPLWSRDGRE
                     LFFQYQTSLMVADIAPGAVFKAGQPRRLYEGRYRTSANGNTPWSISPDGKRFLRIQQA
                     QPDVAIDRIDVVLGWGDQLKRLAAAR"
     gene            complement(1103..>2975)
                     /locus_tag="VFD06_00860"
     CDS             complement(1103..>2975)
                     /locus_tag="VFD06_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02113.1"
                     /translation="PSSPPALDRLVRTCLAKDPDERWQSAADIRRQLSWIGTESGEAP
                     PAAAPGQRVAWLPWMIAAVVAVAAAASLFTVSRSGPAPAGPRMELSIVLPERTLQNDF
                     FQLSPDGTTLAFSGLARGKSMLRLRPLASGAVRELPGTASAESVFWSPDGRSLGYVAR
                     GKLWRIDVATGSIEALADAQSGRGGAWSAQGDILFAAKAAGTIYRVPATGGAVTEATV
                     LEEGDLLHRWPQFLPDGKSFLFFAKTGSRDTTGTYLGSLGDRRRRLVLRNGATGVFLA
                     PATLLFGRGGAILTQAFDPATGELSGSPETVVRPVMRAELGSFIDLFTVSRDGVLVYR
                     AGSAERLLTWMSRDGMVEGTLGHPDVIWSVTLSPDDRQAAISTRTVETGAYTSTLIDI
                     GRNVTTSTVEASAMLMWTPDGRDLLYRSEGRTYEVRRRSVQGGDDVSAGVVDVFATPH
                     SVSPDGRHVLYTRMGGNFDIGVQGLEAGGTPQILLGSEFDERGPRFSADGRLFAYSSD
                     EPGQTEIFVRRFPMTQDVWRISTSGGQQPSWSRDGRELFFVAADGRLMSVAVSSSGRE
                     PSFGPPRPLFRMPVRLNSVVNQYAVSADGRRFLVAVPTEDFDAEPFRVLLNWRAAGP"
BASE COUNT          460 a         1042 c         1060 g          413 t
ORIGIN      
        1 ctatcttctc tatgggcggt tcgacaacct ctttgcgatc ccgtggcagc cgacggtgac
       61 cgacctcagc ggggcggtgc cgatcaccct gccggagcat ccgcggctgg agaacgaagg
      121 gggcgccgcc tacgatgcgg cacccaacgg caccctcgtc taccttcccg gcggcccggc
      181 gcgctacggc cagcgcgtcg tctgggtcga tcgcgatggc cggacggatc cggtgccgat
      241 ccccgagcgc gacctcggca gcgtcgccct ctcgcccgac ggccgacagg cggcggtgca
      301 ggtgaccgag gggaccatcg gcatctggct gtacgacttc gagcgcaaga ccttcacccc
      361 gttcgtgacc gggcccggga gcagtcaggc gccgctctgg actcccgacg gcacgcggct
      421 ggtctaccgc ggcacgcgcc atgggacgcg ggacatctat atcaagccgg ccgacggcac
      481 cggtgccgag acgcggctga cgtccgggct ggggcgctcg ctcacggcga cctcgatgac
      541 tcccgacggg cggtggttga tcttctcggg catcggtgga gcgagcgggg gggagaacat
      601 gatctggagg gtgcccctcg acggcgcggg caagccggag ccgatgctcg ctcccgccga
      661 caggcttcag aacggccaga tctccccgga cggacgcttc atggcgtact cctcggggat
      721 ctccggccgt ccggagatct acgtgatgcc gtttccggga ccggggccga gcaggcagat
      781 ctccatcggg ggcggctacg agccgctctg gtcacgcgac gggcgcgagc tgttcttcca
      841 gtaccagacc agcctgatgg tggccgacat cgcgcccggg gctgtgttca aggcggggca
      901 gccgcgccgc ctctacgagg gccgctatcg caccagcgcc aacggcaaca ccccgtggtc
      961 gatctcaccg gacggcaagc gcttcctgcg catccagcag gcccagcccg acgtggccat
     1021 cgatcggatc gacgtcgtcc tcggctgggg cgatcagctg aagcgactcg cggccgcgcg
     1081 gtgaagcggc ggcgctgagc ggtcagggtc cggcggcccg ccagttcaac aggacgcgaa
     1141 acggctcggc gtcgaagtcc tcggtcggca cggcgacgag gaagcgccgt ccgtcggccg
     1201 acaccgcgta ctgattcacc acactgttca ggcgcacggg catacggaag agcggtcgcg
     1261 gcggtccgaa cgacggttcc cgtcccgaag acgacaccgc aacggacatg aggcggccgt
     1321 cggctgcgac gaagaacagc tcgcggccgt cgcggctcca ggacggctgc tgccccccgc
     1381 tggtggagat gcgccagacg tcctgtgtca tcgggaagcg gcgcacgaaa atctccgtct
     1441 gtcccggctc gtcggagctg tacgcgaaga ggcgcccatc ggcggagaaa cgcggcccgc
     1501 gctcgtcgaa ctcgctcccc aggaggatct gcggcgtgcc acccgcttcc aggccttgca
     1561 cgccgatgtc gaagttgcca cccatgcgcg tgtacaggac atggcggccg tcgggcgaga
     1621 cggaatgcgg tgtcgcgaag acgtccacga ctcctgccga gacgtcgtcg cctccctgca
     1681 ccgagcggcg ccgtacctcg tacgtacgcc cctcgctgcg atacagcagg tcgcgtccgt
     1741 cgggggtcca catcaacatc gccgatgcct ccacggtcga ggtggtgacg ttgcgaccga
     1801 tgtcgatgag cgtgctcgtg taggcgccgg tctccacggt gcgggtggag atcgccgcct
     1861 gccggtcgtc gggagacagc gtcacgctcc agatgacgtc gggatgcccg agcgttcctt
     1921 cgaccatccc gtcgcggctc atccaggtca ggagccgctc ggcgcttccg gctcgataga
     1981 cgaggacgcc gtcgcgggac accgtgaata ggtcgatgaa cgacccgagc tcggcgcgca
     2041 tcactggtcg gacgacggtc tccggcgacc ccgacagctc gccggtagcc gggtcgaacg
     2101 cctgcgtcag gatggcgccg ccgcggccga acaggagggt cgctggggcg aggaagacgc
     2161 cggtggcgcc gttgcgcagg acgagcctcc gccgtcgatc gccgagcgat ccgaggtagg
     2221 tgccggtggt atcgcggctt ccggtcttgg cgaagaagag gaagctcttg ccgtccggca
     2281 ggaactgcgg ccagcggtgc agaaggtccc cctcctcgag gacggtcgcc tccgtgacgg
     2341 cgccgccggt cgccggcacg cggtagatag tgcccgccgc cttcgcagcg aagagaatgt
     2401 ccccctgcgc cgaccaggcc ccgccgcggc cgctctgggc gtctgcgagg gcctcgatcg
     2461 atccggtggc cacatcgatg cgccacagct tgccgcgcgc cacgtagccg agcgacctgc
     2521 cgtccggcga ccagaagacc gactcggctg aggcggttcc cggcagctcg cggaccgcgc
     2581 ccgaggcgag cgggcgcagc cggagcatcg acttccccct cgcgagcccc gagaacgcca
     2641 gcgtcgtccc gtccggcgac agctggaaga aatcgttctg cagggtgcgc tccggcagca
     2701 cgatcgacag ctccatccgc ggcccggcgg gtgccggccc ggagcgcgac acggtgaaga
     2761 gcgacgcggc cgccgcgacc gccacgacgg cggcgatcat ccaggggagc cacgcgacgc
     2821 gttgcccggg ggccgcagcg ggcggggcct caccgctctc ggtcccgatc cacgagagct
     2881 ggcgccggat gtcggcggcg ctctgccagc gctcgtcggg atccttggcg agacaggtgc
     2941 ggaccaggcg atcgagcgcg ggcggggacg acggt
//
LOCUS       DASRIQ010000033         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1317904, whole genome shotgun sequence.
ACCESSION   DASRIQ010000033 DASRIQ010000000
VERSION     DASRIQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317904"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFD06_00865"
     CDS             complement(<1..319)
                     /locus_tag="VFD06_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZN02114.1"
                     /translation="MQFEKLHPPAEGNPLRYEDGRCVAPSDPILPFIEGDGVGPEIVA
                     VARRVLDAAVERAYGRERRLVWFELYAGGKAWERYGEWLPQDTLDAISHFGIALKGPL
                     TTPV"
     gene            complement(325..1131)
                     /locus_tag="VFD06_00870"
     CDS             complement(325..1131)
                     /locus_tag="VFD06_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02115.1"
                     /translation="MKTLGILLAFLKRDLLVEASYRTSFVLQFTGIIMSVLIWRFVSL
                     VMKAPADTPGLEGMDYFAYVLLGLSFFHFLSSALASFSQKIRQEQMTGTLEAILVTPT
                     SPGTVVLGSALWEFVMASVKVAIYLAVGWGFGVAIRFENLPLCLVVLAITVLAFSGIG
                     ILSAAFIFYLKRGDPITYFVSSASVLLGGVFYPPQDMWPWLETLSRFLPVTYALHAWR
                     HALLKGATFRDLLPDIAALLVFVAILLPIGALAFRFAIRKARQEGSLVQY"
     gene            complement(1128..2159)
                     /locus_tag="VFD06_00875"
     CDS             complement(1128..2159)
                     /locus_tag="VFD06_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN02116.1"
                     /translation="MATAAAPEASRASASPALRLTGVGKTYIKRRSLLEMALHPWRRA
                     ERVEGLVDVDLEVPRGMLYGLLGPNGAGKTTLLKILACLILPTQGKAEVEGRDVATED
                     QEVRRVIGFVTSDERSFYWRVSGEDNLMFFARLYGLSAAAARRRTRDLLAAMELEEVA
                     KRQFMSYSSGMKQRLAICRALLHDPPVLCLDEPTRSLDPIAAKHLRQFIRQSLHRERG
                     KTILLATHNLQEAEDLCQRLAVLHRGRVLREGALHEIAARLPGREQYLIEVQGVAALP
                     NDPRWSLDPEPPRDGLVRLAAAVDRGGRPFSDLLQALLAGGGTIVACTRREPGLQEVF
                     DQMEREGVR"
     gene            complement(2159..>3628)
                     /locus_tag="VFD06_00880"
     CDS             complement(2159..>3628)
                     /locus_tag="VFD06_00880"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HZN02117.1"
                     /translation="AWQRREVTAGSGYLSSSAVPVHAGLGSRSLADVVRLLWPGGVLQ
                     AELDVSAGQRIEPQELDRKGSSCPLLFAWDGGSWRFITDILGVGGLGLWLAPGKYGTP
                     VPDESLLITAKDLQPLEGAYYFQVLENLEEVTYLDAARLQVVDHPKDVEVYPNMNFGG
                     PPRGTPRLYAFERAARIFPSRAVDHHGRNVTDRLLKVDRTYPDEFRKLSFAGYAEMHD
                     VTLEFPPSIVDQEGFVLFLNGWTDFEYSSSNYAAFQQELTMTLPILEMEDADGVFKPV
                     LEPMGFPPGMARSIAVDLSSLGPLKGRRMRLRTNMRVYWDQIFLARPWDEATMSARIQ
                     VSDAKAAAAHLHRRGFPREHKPDGKEPRLYDYSIMDNTQPFRTIAGDYTRFGRVTDLV
                     TEADDRAVIFGKGEELTLEFPVKGLPQQPKGTIRSFVLHLSGWCKDMDPHTAHGETVE
                     PLPFRGMKSYPYAEGETYPDDPEHRGWRSTWNTRRLEGR"
BASE COUNT          608 a         1230 c         1275 g          515 t
ORIGIN      
        1 cgaccggcgt cgtcagcggc cccttgagag cgatgccgaa gtgcgagatg gcgtcgaggg
       61 tgtcctgggg gagccactcg ccgtatcgct cccaggcctt gccgccggcg tacagctcga
      121 accagacgag gcgacgctcg cggccgtagg cccgctcgac cgccgcgtcc agcacccgcc
      181 gcgccaccgc gacgatctcc ggccccaccc cgtccccctc gatgaagggc aggatcgggt
      241 cggagggggc gacgcagcgc ccgtcctcgt agcgcagggg attcccctcg gccgggggat
      301 gcagcttctc gaattgcatg agcgtcagta ctgtaccagc gaaccttcct ggcgcgcctt
      361 gcggatcgcg aagcggaacg ccagagcccc gatcggcaaa aggatcgcca cgaagacgag
      421 gagggcggcg atgtccggga ggaggtcccg gaaggtcgcc cccttcagga gcgcatgccg
      481 ccaggcgtgc agggcgtagg tgaccggcag gaagcgcgac agcgtctcga gccagggcca
      541 catatcctgg ggggggtaga agacgccgcc gagcagcacc gaagcgctcg acacgaagta
      601 ggtgatcgga tcgccgcgct tcagatagaa gatgaacgcc gccgacagaa tgccgatgcc
      661 ggagaaagcg agcacggtga tcgccaggac gaccaggcag agcggcaggt tctcgaagcg
      721 gatcgccacc ccgaaccccc agccgacggc gaggtaaata gcaactttca cggatgccat
      781 cacgaactcc cacagcgccg acccgagcac gaccgttccc ggggaggtcg gggtgaccag
      841 gatcgcctcg agcgttccgg tcatctgttc ctggcggatc ttctgcgaga acgacgccag
      901 cgccgacgac aaaaagtgga agaacgacag gccgagcagg acgtacgcga agtagtccat
      961 cccctcgagc cccggcgtgt cggccggtgc cttcatcacc agcgacacga agcgccagat
     1021 gaggaccgac atgatgatcc cggtgaactg caggacgaaa ctcgtcctgt aggaagcctc
     1081 gaccagcagg tcgcgcttca ggaacgcgag caggatgccg agggttttca ccggacccct
     1141 tcccgctcca tctggtcgaa gacctcctga agcccgggct cgcggcgcgt gcaggccacg
     1201 atcgtcccgc ccccggcgag cagcgcctgc agcagatcgg agaagggccg gccgccgcga
     1261 tcgaccgcgg cggcgaggcg cacgaggccg tcgcgaggcg gctccggatc gaggctccag
     1321 cgcgggtcgt tcgggagggc cgcgactccc tggacctcga tgaggtactg ctcccgtccc
     1381 ggcagccggg ccgcgatctc gtgcagcgcc ccctcgcgca gcacccgccc gcggtgcagg
     1441 accgccagcc gctggcagag gtcctccgcc tcctgcaggt tgtgggtggc gagcaggatc
     1501 gtcttgccgc gctcccggtg caggctctgg cggatgaact ggcgcaggtg cttggcggcg
     1561 atcggatcga gcgagcgcgt cggttcgtcg agacagagga cgggagggtc gtgcaggagc
     1621 gcccggcaga tcgccagccg ctgcttcatc ccggacgagt acgacatgaa ctggcgcttc
     1681 gcgacctctt cgagctccat ggccgcgagg aggtcgcggg tccgccggcg ggccgccgcc
     1741 gccgagagcc cgtagaggcg ggcgaagaac atgaggttgt cctcccccga aacgcgccag
     1801 tagaacgagc gctcgtcgga ggtgacgaag ccgatgacac gccgcacctc ctggtcctcg
     1861 gtcgcgacgt cccgtccttc cacctccgcc ttcccctgtg tcggcaggat gaggcaggcg
     1921 aggatcttga ggagggtcgt cttgccggcg ccgttcgggc cgagcaggcc gtagagcatg
     1981 ccgcgcggca cctcgagatc gacatcgacg agcccctcga cccgctcggc acgacgccac
     2041 ggatggagcg ccatctccag gagcgagcgg cgcttgatgt aggtcttccc caccccggtc
     2101 aggcggagcg ccgggctcgc ggaggcccgg gatgcctcgg gggcggcagc cgtggccatc
     2161 agcgcccctc gagccgccgg gtgttccagg tggatcgcca gccccggtgc tccgggtcgt
     2221 cggggtacgt ctccccctcg gcgtacggat aggacttcat gccccggaac ggcagcggct
     2281 ccaccgtctc gccgtgcgcc gtgtgcgggt ccatgtcctt gcaccagccg ctcaggtgga
     2341 ggacgaagga gcggatcgtc cccttcggct gctgcgggag ccctttgacc gggaactcca
     2401 gcgtcagctc ctcccccttg ccgaagatga cggcacggtc gtcggcttcg gtgacgagat
     2461 cggtcacgcg cccgaagcgg gtgtagtcgc cggcgatggt ccggaacggc tgcgtgttgt
     2521 ccatgatgct gtagtcgtac aggcgcggct ccttcccgtc cggtttgtgc tcgcgcggga
     2581 agccgcggcg gtgcaggtgg gccgcggcgg ccttcgcgtc ggacacttga atgcgcgccg
     2641 acatcgtcgc ctcgtcccac ggccgggcga ggaagatctg gtcccagtac acccgcatgt
     2701 tggtgcggag gcgcatgcgc cgccccttga gcggcccgag cgaggagaga tcgacggcga
     2761 tcgaccgcgc catcccgggg gggaagccca tcggctcgag gaccggcttg aacacgccgt
     2821 cggcgtcctc catctcgagg ataggcagcg tcatggtcag ctcctgctgg aacgcggcat
     2881 agttgctcga cgagtactcg aaatcggtcc agccgttgag gaacaggacg aacccttcct
     2941 ggtcgacgat cgacggcggg aactccagcg tcacgtcgtg catctcggcg tagccggcga
     3001 agctcagctt ccggaattca tcgggatagg tgcggtccac cttgagcagc cggtcggtca
     3061 cgttgcggcc gtggtgatcg accgcacgcg atgggaagat gcgcgccgcc cgctcgaagg
     3121 cgtacagccg cggcgtcccg cgcggcggcc ccccgaagtt catgttgggg tagacctcga
     3181 cgtccttcgg gtgatcgacc acctggaggc gcgcggcgtc gaggtaggtc acctcctcga
     3241 ggttctcgag gacctggaag tagtacgccc cctcgagcgg ctgcaggtcc ttggcggtga
     3301 tgagaagcga ctcgtcaggc accggcgtac cgtatttccc gggcgccagc cacagcccga
     3361 ggccgccgac gccgaggatg tcggtgatga accgccagga tccgccgtcc caggcgaaga
     3421 gaagcgggca ggaggagccc ttgcgatcca gctcctgggg ctcgatccgc tggcccgcgg
     3481 agacgtccag ctccgcctgc aggacacccc ccggccacag cagccggacg acgtcggcaa
     3541 gcgagcggga cccgaggccg gcgtgcaccg gcaccgcgga cgaggacagg tatccggagc
     3601 cggcggtcac ctcgcggcgc tgccaggc
//
LOCUS       DASRIQ010000034         6650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3396694, whole genome shotgun sequence.
ACCESSION   DASRIQ010000034 DASRIQ010000000
VERSION     DASRIQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3396694"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VFD06_00885"
     CDS             complement(<1..1137)
                     /locus_tag="VFD06_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02118.1"
                     /translation="MIDDVDGGDVIRPTGRFFSGLDAGPTPIPGLPATPVALPIPVTA
                     GDVGPIPSPAPQRVHVEAIRVSDLPDFARAALADRVRYAVLPIPPVRAEAQAKNPLAQ
                     PDDVGLLVGYVGDRCAGFLGLLPGAIEFRGRRRPVSSLSGFYVAPGDRTSGLVTRLPL
                     AAIALGRDLFVSRISQESYALFTQLGFWPARPRRTLRLQLSRYKKLKGIPLRMLGRTV
                     DATGSQEKWRLVTEARVPMTASWIDGESEAAGPDAAAGAAPSGPAADPAERPRFVRDE
                     AVINWMVRHPWIGEDSHLQLPYTLSYRREMFRYMVFEANAGGYMVLNVTQDQRFATLR
                     LLDRRPMTDALREEALARALLEAQRLVADVVECPPEYEPLLRRSF"
     gene            1259..2281
                     /locus_tag="VFD06_00890"
     CDS             1259..2281
                     /locus_tag="VFD06_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZN02119.1"
                     /translation="MPPRTTKPRSRMVIYLPHPLPIWRIPHSSIERIRKAAGRLYDID
                     LPRDERELERAIPETEVLYAWGLTGKLVPLAGRLSWLHTPLSGVDRVLNPELLKTSIR
                     VTSSRGVNSVAVAEHTFGMVLALTRGIAQAARAQAERRFIQGELYGRKPPLAELNGKV
                     LGLYGFGEIGREIASRAHAFGMKVWAVSRSANAKTKPPHGVDRLLPAKRAEAMVRAAD
                     VLVLALPLTEATRGIAGERLLHSMKPTAILVNIGRGALVQEPALSRAMREGWIAGAAL
                     DVFVHEPLPVESPLWGLPNVLLTPHVAGTHPDYMARSAGIFLDNLKRYRAGKPLLNQV
                     NLQAGY"
     gene            complement(2303..2776)
                     /locus_tag="VFD06_00895"
     CDS             complement(2303..2776)
                     /locus_tag="VFD06_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02120.1"
                     /translation="MAPGIDPAGRTFRRSLSARVVSVTALVLFGLAVATRTVSGDLGA
                     GYFILVALVLVSIAGMVSAWGDRITIDAAGVETRNVLLARLPGFGRRSRRRLGWNDIL
                     RVQEHRRPGSDPKDPPRALFLVPVRGRRLALDSIESFDQVLALVRRAMSDRSSAT"
     gene            complement(2761..3114)
                     /locus_tag="VFD06_00900"
     CDS             complement(2761..3114)
                     /locus_tag="VFD06_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZN02121.1"
                     /translation="MTGQPDCLFCKIGRKEIPARIAYEDRQVVAFEDISPQAPHHTLI
                     IPKAHFATFNDVGAGEESLLGHMMVVASRIAQEKGLDKEGFRLVANCLESAGQSVFHI
                     HLHLLGGRRFAWPPG"
     gene            complement(3111..4394)
                     /gene="murA"
                     /locus_tag="VFD06_00905"
     CDS             complement(3111..4394)
                     /gene="murA"
                     /locus_tag="VFD06_00905"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZN02122.1"
                     /translation="MDKIRIEGPCRLSGTVRASGAKNAVLPEMAAFLMLEGPVTLKNV
                     PLVRDVTTMGLVLRHLGIRDLVFDGGVLTASGCEPKEPEAPWNLVRTMRASILVLGPL
                     LARLGRAKVSLPGGCAIGARPIDLHIEALRKMGARIAIEHGYVLAECPRLRGAEIDFP
                     KPTVTGTENLMMAAALAEGRTILRGCALEPEIGDLAVFLNACGAKIAGAGTGTITIDG
                     VAKLRGAEHRAIPDRIEAGTFLVATAATGGEVTVEGAEPAHLAALLEALRAAGARVEL
                     HPAGTKPEERRTSMSVVPDGPLRATSVTTLPHPAFPTDLQAQFMALLTAATGVSTVTE
                     TVFENRFMHVGELRRMGAKIEVDGETATVTGPTPLSGAPVMASDLRASACLIVAGLLA
                     EGETLIHRAYHIDRGYEQVVQKFRALGARIERIRE"
     gene            complement(4435..6453)
                     /locus_tag="VFD06_00910"
     CDS             complement(4435..6453)
                     /locus_tag="VFD06_00910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02123.1"
                     /translation="MAAILAVLLALAPQPAQAEVTPDGLPVESDHWIRLQAPHFLIFS
                     DATPERTVELARQIETFRAALLRLGTGLEARSTLQTVVFIFKDHETYRPYRHRSDIAG
                     MFVKHRDGNSIAVNASAPGGPWQIVFHEYMHFFLNNNFTDIPLWLDEGMAEAYSTFTL
                     KGTTARLGAPLDAQRRWLESHPLMPLETLFRVDHGSSEYQEESKVGTFYAQSWALVHY
                     LAFGGGAAVAAPGGGGGAAGGADKPDAAAFFRNVKRGGSLRQYVVPMLLNDRGRLIDR
                     LQRYIAKDDYAETVLDLGRLDAIDLPATTPVPRAELLYRLGDYLLHLDKRTKQAERHL
                     YEAVRLNPKHSRAWAALGQLEHQRDRFKEASALFDKALLLAPDDPSIALLYAYSLVER
                     TFPPGVVVRKDVGEVPKTLLQARALFKKALAKSPDIVEAWAGLGSTYAFDSGNVGPGI
                     AALEKALLLAPGRVDVAIDLAELYVRSGKKQRAEDLVERIVRHSSDPQQRAVGEALLL
                     RAEVVEAQERVQKGDIEGGLAALRNLRARAPSDKERDEIDGILTSLERTSTSNFFINL
                     FNDGAEKFNARDFDAAISLFEQVAKECPDPDLAKEARQRLELARKSQAEERHVAWYND
                     AVEKYKRRDYKGAAALLRQVLDDSPDERIGAAARDLLSKVRAAAPGVK"
     gene            6337..>6650
                     /locus_tag="VFD06_00915"
     CDS             6337..>6650
                     /locus_tag="VFD06_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZN02124.1"
                     /translation="MRGLQPDPVVRFDRQPVGRDLRLRRLGREREQHGQDRGDAGHEA
                     ICTTLRIIHARFTGGAVPLYEYECKKCHFRFERIQLFSDPLVRKCPKCKTGRVEKLLS
                     APA"
BASE COUNT          972 a         2216 c         2466 g          996 t
ORIGIN      
        1 gaacgaccgg cgcaggagcg gctcgtactc gggggggcac tcgacgacgt cggcgaccag
       61 gcgctgcgcc tcgaggaggg cgcgcgccag cgcctcctca cgcagcgcgt cggtcatcgg
      121 gcggcggtcg aggaggcgca gggtcgcgaa gcgctggtcc tgggtgacgt tcaggaccat
      181 gtagccgccg gcgttcgcct cgaacaccat gtagcggaac atctcgcgtc ggtacgacag
      241 ggtgtacggc agctggaggt gcgagtcctc gccgatccag ggatggcgca ccatccagtt
      301 gatcaccgcc tcgtcgcgga cgaagcgggg gcgctcggcg gggtcggctg cggggccgct
      361 cggcgccgca cctgccgccg cgtcgggccc cgcggcctcg ctctcgccgt cgatccagga
      421 ggcggtcatc gggacgcgcg cctcggtcac gagccgccac ttctcctggc tcccggtggc
      481 atcgacggtg cgccccagca tccgcagcgg gatccccttg agcttcttgt agcgcgacag
      541 ctgcaggcgc agggtgcgcc gcggccgggc gggccagaag ccgagctgcg tgaacagggc
      601 gtaggactcc tggctgatcc ggctgacgaa caggtcgcgg cccagcgcga tggcggcgag
      661 cggcagcctg gtgacgaggc cgctggtgcg atcgccgggc gcgacgtaga agccggagag
      721 gctcgacacc gggcgccggc ggccgcggaa ctcgatggcg ccggggagga gccccaggaa
      781 cccggcgcaa cgatcgccca cgtatccgac cagcagaccg acgtcgtcgg gctgcgccag
      841 cgggttcttc gcctgcgcct cggcgcgcac cggcgggatc ggcagcaccg catagcggac
      901 acggtcggcg agggcggcgc gggcgaagtc cggcaggtcg gacacgcgga tcgcttcgac
      961 gtgcaccctc tgaggggcgg gggaggggat cgggccgacg tcgccggccg tcaccggtat
     1021 cggcagcgcc acgggcgtgg ccggcagccc ggggatcggg gtggggccgg cgtcgagccc
     1081 gctgaagaac ctcccggtgg gccttatgac gtcacctccg tcaacgtcat caatcatccc
     1141 cgactcctgg aagttggaca aggaagaagc ggtttctacg gagggtatct ccggcacgga
     1201 gtatatccgg gggtcgtcgt agaatgcgcg gccggaccgt tcggccgagg accactgcat
     1261 gcctcccagg accacgaagc cgcgctcacg gatggtgatc tacctgcccc acccgctgcc
     1321 gatctggcgc ataccccact cgtcgattga acgcatccgc aaggcggccg gcaggctgta
     1381 cgacatcgac ctgccgcgcg acgagcggga gctggagcgg gccatcccgg agaccgaggt
     1441 gctgtacgcc tgggggctga ctggaaagct ggtcccgctc gcggggcggc tgagctggct
     1501 gcacacgccg ctctccgggg tggaccgggt gctcaacccg gagctgctga agacgtcgat
     1561 ccgggtcact tcgtcgcgcg gggtcaactc ggtcgcggtg gcggagcaca ccttcgggat
     1621 ggtgctggcc ctgacgcgcg gcatcgcgca ggcggcgcgg gcgcaggcgg agcgccgctt
     1681 catccagggg gagctgtacg gccggaagcc tccgctcgcg gagctgaacg gcaaggtgct
     1741 gggactctac ggcttcgggg agatcggccg ggagatcgct tcgcgcgcgc atgccttcgg
     1801 gatgaaggtc tgggcagtgt cccggtccgc caacgcgaag acgaagccgc cgcacggcgt
     1861 cgatcgcctg ctgccggcga agcgggccga ggcgatggtg cgggcggcgg acgtcctcgt
     1921 cctcgcgctg ccgctgaccg aggccacgcg cggcatcgcc ggcgagcggc tgctgcactc
     1981 gatgaagccg accgccatcc tcgtgaacat cggccgcggc gcgctcgtcc aggagccggc
     2041 cctgtcgcgc gccatgcggg aagggtggat cgccggcgcg gcgctcgacg tcttcgtgca
     2101 cgagccgctg ccggtcgaga gcccgctctg ggggctgccg aatgtcctgc tgacgccgca
     2161 tgtggcgggg acccaccctg actacatggc ccggtcggcg gggattttcc tggacaacct
     2221 caagcggtac cgggcaggga agccgctcct gaaccaagtc aatctgcagg ccggatattg
     2281 accgtcgcgc cgcgtccgcg gcctaggtcg cggacgagcg gtcgctcatc gcgcggcgga
     2341 cgagcgcgag aacctggtcg aagctctcaa tcgagtcgag cgccagcctc ctccccctga
     2401 ccggcaccag gaacagggcc cgcggcggat cctttggatc ggagccgggg cggcggtgtt
     2461 cctgcactct caagatgtca ttccacccga gacggcgccg ggagcgacgg ccgaatcccg
     2521 ggagacgcgc caggaggaca ttgcgcgtct cgacgccggc ggcgtcgatg gtgatgcggt
     2581 ccccccacgc gctgaccatg ccggcgatcg acaccagcac gagggcgacg aggatgaaat
     2641 agccggcgcc caaatctccg gagacggtgc gggtcgcgac cgcgaggccg aacaggacga
     2701 gggcggtcac gctgaccacc cgcgccgaca ggctgcgccg gaaggtccgg cccgcggggt
     2761 ctatcccggg ggccacgcga accggcggcc gccgagcagg tgcaggtgga tgtggaagac
     2821 cgactggccg gcgctctcca ggcagttggc gacgaggcgg aacccctcct tgtcgagccc
     2881 cttctcctgc gcgatgcgcg acgcgaccac catcatgtgg ccgaggagcg attcctcgcc
     2941 ggcgccgacg tcgttgaagg tggcgaagtg cgccttcggg atgatcagcg tgtggtgcgg
     3001 cgcctgcggg ctgatgtcct cgaacgccac cacctgccga tcctcgtagg cgatgcgggc
     3061 cgggatctcc ttccggccga tcttgcagaa caggcaatcc ggttgaccgg tcattcgcgg
     3121 atcctttcga tgcgtgcgcc gagggcgcgg aacttctgga ccacctgctc gtagccgcgg
     3181 tcgatgtggt aggcgcggtg gatgagggtt tccccttcgg cgaggaggcc ggcgacgatg
     3241 aggcaggcgg aggcgcgcag gtcgctcgcc atcaccggcg cgccggagag gggggtcggg
     3301 ccggtcaccg tcgcggtctc gccgtccacc tcgatcttcg cccccatgcg gcgcagctcg
     3361 ccgacgtgca tgaagcggtt ctcgaagacg gtctccgtga ccgtcgaaac acccgtggcc
     3421 gcggtcaaaa gcgccatgaa ctgcgcctgc aggtcggtcg ggaaggcggg atgcggcagg
     3481 gtcgtgacgc tggtggcgcg cagcgggccg tcgggaacga ccgacatcga ggtgcggcgc
     3541 tcctccggct tcgtgccggc cgggtgcagc tcgacgcgcg ccccggcggc gcgcagcgcc
     3601 tcgaggagcg ccgcgagatg cgccggctcg gccccttcca cggtgacctc gccgcccgtg
     3661 gcggcggtgg cgacgaggaa ggtgccggcc tcgatccgat ccgggatggc gcggtgctcg
     3721 gcgccgcgca gcttcgcgac gccgtcgatg gtgatggtgc ccgtgccggc gccggcgatc
     3781 ttcgcgccgc aggcgttgag gaacacggcg aggtcgccga tctccggctc gagggcgcag
     3841 ccgcgcagga tggtgcgccc ctcggcgagg gccgccgcca tcatgaggtt ctcggtgccg
     3901 gtgaccgtcg gcttcgggaa gtcgatctcg gcgccgcgga ggcgcgggca ctccgccagg
     3961 acgtagccgt gctcgatggc gatgcgcgcc cccatcttcc gcaacgcctc gatgtgcagg
     4021 tcgatcgggc gggcgccgat ggcgcagccg ccggggagtg agaccttggc gcgcccgagg
     4081 cgcgccagga gcggcccgag caccaggatc gaggcgcgca tggtgcgcac caggttccag
     4141 ggagcctccg gctccttcgg ctcgcacccg gaggcggtga gcacgccgcc gtcgaagacc
     4201 aggtcgcgga tgccgagatg ccgcaggacg agccccatcg tcgtgacatc gcgcaccagc
     4261 ggcacgttct tcagcgtgac cgggccttcg agcatcagga aggcggccat ctcggggagg
     4321 acggcgttct tggcgccgct ggcgcggacg gtgccggaga gacggcaggg gccttcgatg
     4381 cggatcttgt ccatggcggt cgcgattata gatggacggc ccggacaggg cccgtcactt
     4441 cacgcctggt gcggcagcgc gcaccttcga gagcagatcg cgggccgccg cgccgatccg
     4501 ctcgtcgggc gagtcgtcga gcacctgccg cagcagcgcc gcggccccct tgtagtcccg
     4561 ccgcttgtac ttctcgacgg catcgttgta ccaggcgacg tgccgttcct cggcctgcga
     4621 cttgcgggcg agttcgaggc gctggcgcgc ctccttcgcg agatcggggt cggggcactc
     4681 cttcgcgacc tgctcgaaga gcgagatggc ggcgtcgaaa tcgcgggcgt tgaacttttc
     4741 cgcgccgtcg ttgaacaggt tgatgaagaa gttggaggtc gaggtccgct cgaggctggt
     4801 gaggatgccg tcgatctcgt cccgttcctt gtcggagggg gcgcgggcgc gcaggttcct
     4861 gagcgccgcg agcccgcctt cgatgtcccc tttctgcacc cgttcctgtg cctcgacgac
     4921 ctcggcgcgg agcaacagcg cctcgcccac ggcccgctgc tgcgggtcgc tgctgtggcg
     4981 gacgatccgt tcgacgaggt cctcggcccg ctgctttttc ccggagcgga cgtacagctc
     5041 ggcgagatcg atcgccacgt ccacacggcc gggggcgagg aggagcgcct tctccagcgc
     5101 ggcgatgccc gggcccacgt tgccgctgtc gaacgcgtag gtggagccga gcccggccca
     5161 ggcctcgacg atgtcgggac tcttcgcgag ggctttcttg aagagcgcgc gcgcctgcag
     5221 gagcgtcttc ggtacctccc ccacgtcctt ccgcacgacg acccccggcg gaaaggtgcg
     5281 ctcgacgagg ctgtaggcgt agaggagcgc gatcgacggg tcgtcgggag cgaggaggag
     5341 cgccttgtcg aacagcgcgc tcgcctcctt gaagcggtcg cgctggtgct cgagttgtcc
     5401 gagggccgcc caggcgcggc tgtgtttggg attgaggcgg accgcttcgt agaggtgccg
     5461 ctccgcctgc ttggtccgct tgtccaggtg cagcaggtaa tcccccagcc ggtacagcag
     5521 ctccgcccgc ggcaccggag tggtcgccgg gaggtcgatc gcatcgagcc ggccgagatc
     5581 gaggaccgtc tcggcatagt cgtccttcgc gatgtagcgc tgcaggcgat cgatgagacg
     5641 gccccggtcg ttgaggagca tcggcaccac gtactgacgc agcgacccgc cgcgtttgac
     5701 gttgcggaaa aacgcggccg cgtccggctt gtcggcgccg ccggccgcac caccgccacc
     5761 cccaggcgcg gcaactgccg cgccgccacc gaaggcgagg tagtggacca gcgcccacga
     5821 ctgggcgtag aaggtcccga ccttggactc ctcctggtac tcgctcgagc cgtggtcgac
     5881 gcggaacagg gtctcgagcg gcatcagggg atggctctcg agccagcggc gttgggcgtc
     5941 gagcggggcg ccgaggcgcg ccgtcgtccc cttcagggtg aaggtgctgt acgcctccgc
     6001 catcccttcg tcgagccaca gcgggatgtc ggtgaagttg ttgttcagga agaagtgcat
     6061 gtactcgtgg aacacgatct gccacgggcc tcccggggcg ctggcgttga ccgcgatgct
     6121 gttgccgtcg cggtgcttga cgaacatgcc ggcgatgtcg gagcgatggc ggtacggccg
     6181 gtaggtttcg tggtccttga agatgaagac gacggtctga agcgtggatc gggcctcgag
     6241 ccccgtgccg agcctgagga gggcggcgcg gaacgtttcg atctggcggg ccagctcgac
     6301 ggtccgctcg ggcgtcgcgt cgctgaagat caggaaatgc ggggcctgca gccggatcca
     6361 gtggtccgat tcgaccggca acccgtcggg cgtgacctcc gcctgcgccg gctggggcgc
     6421 gagcgcgagc agcacggcca ggatcgcggc gatgcggggc acgaagcgat ctgtaccacg
     6481 ttgcgtataa tccacgctcg cttcacggga ggtgcggtgc cgctttacga gtacgagtgc
     6541 aagaagtgcc attttcgctt cgagcggatc cagttgttca gcgatccgct ggtgcgcaag
     6601 tgtcccaagt gcaagaccgg ccgcgtcgag aagctcctct ccgcccccgc
//
LOCUS       DASRIQ010000035         5195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3663843, whole genome shotgun sequence.
ACCESSION   DASRIQ010000035 DASRIQ010000000
VERSION     DASRIQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3663843"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFD06_00920"
     CDS             complement(<1..589)
                     /locus_tag="VFD06_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZN02125.1"
                     /translation="MIYDLTPPIDRALKVFPGDTPPEREVLLDMEAGAHLTLSTLRTT
                     VHVGAHADAPSHYGRGAAPIDARPLDLYLGPCQVLRVPARRGALLGPDDLGGASIAAP
                     RVLLATGTFPDPTVFNEDFAALSPRLIDHLHDAGARLVGVDTPSLDPCHSKDLPAHKA
                     CLRHDMAILEGLLLGHVPEGVYELIALPLRIAGADG"
     gene            complement(620..1444)
                     /locus_tag="VFD06_00925"
     CDS             complement(620..1444)
                     /locus_tag="VFD06_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HZN02126.1"
                     /translation="MTKPKSGSRGGGAPLPLTYARYLRLDALLALQQPASDPPEHDET
                     LFIVIHQVYELWFRLLLHEFEKIRRDFTAGDLFGAIATWKRVRMVLKTLVAQIDVLET
                     MTPMSFLTFRDRLEAASGFQSVQFRELEFLLGNKRPEMIERLPQGDPARAVLERRLKE
                     RSLVDHFYDFLAGLGVAVPEALRRRDVRESNQPDAEIQEGLLRLYKERPDVANLLELM
                     TDFDEGLQEWRYRHVMMVQRTIGNKMGTGGSLGVDYLRKTLFQPIFPDLWLIRNRL"
     gene            complement(1441..2802)
                     /locus_tag="VFD06_00930"
     CDS             complement(1441..2802)
                     /locus_tag="VFD06_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN02127.1"
                     /translation="MSTIIQIGAGLGGTLASIFLGRAGHDVRGFELRPDPRRGPLVGG
                     RSINLALSARGLGALEKAGLLDRVLALAVPMRGRMIHDLRGHTAFQPYGTERGHANHS
                     VSRADLNVLLLEAAEREKNVRFAFGRRCVDIDPDAPSATFAPAEGTGPDGGAGGRMDA
                     PGDTVRGDVVLGTDGAWSVVRRRLQRLDRFDYDQSFLSHGYKELTIPPGPKGSFLLER
                     EALHIWPRGGFMMIALPNRDGSFTCTLFWPHAGPNSFGALADGSAVTRFFERHFPDAL
                     PLFPDLPEQYVTNPVGSMVTIRCRPWHVRGKAALLGDAAHAVVPFHGQGANCAFEDAV
                     VLDECVRAHAPDWETVFDLFESRRKEHTDALARLSVDNFVEMRDHTASRLFRLRKGLE
                     KGLYRLLPGLFMPLYMMISFTRIPYAETVRRARRQALTLQALAAGIGAAAVVAVAWYW
                     RRR"
     gene            complement(2834..4105)
                     /gene="kynU"
                     /locus_tag="VFD06_00935"
     CDS             complement(2834..4105)
                     /gene="kynU"
                     /locus_tag="VFD06_00935"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HZN02128.1"
                     /translation="MNGPALDEEEVHRLDLEDPLSRFRSRFALPAGPSGRPLLYFCGH
                     SLGPMPKEARTLVNEEMDDWEALGVEGHFRSRRPWYSYHERFRETGARLVGARPGEVV
                     MMNGLTVNLHLMLATFYRPTDERHAILIEEHAFPSDQYAVASQIRHHGRDPDASLLIA
                     RHRTGEPGLRTEDLERLIQERGSSIAVVLLPGVQYLTGQALDLERLAAAAHRHGCVFG
                     ADLAHAAGNLPLRLHEWRVDFAVWCSYKYLNAGPGAVAGCFVHERHGLDRSLPRFAGW
                     WGTDPATRFIMPPEFVPRPGADGWQIGNPPILALAPLLASLQIFEEAGLEALRARSVR
                     MTSYLLALLDRLPPGRVEVLTPRRPDERGCQISLRVRDGRAKPLLEALHREGVVLDFR
                     EPDVLRLAPVPLYNTFHEIWRFARVLEQTLA"
     gene            complement(4134..5153)
                     /locus_tag="VFD06_00940"
     CDS             complement(4134..5153)
                     /locus_tag="VFD06_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN02129.1"
                     /translation="MSTPFRVDLHTHILPPRLPDVRERFGYGGFVTLEPAEAGARMMV
                     DGTLFRDVPASTFDAPRRLEECDRKGVAVQVLSTVPVMFCYWAEAEHALEVARLLNDH
                     IAETVGAHPRRFVGLGTVPLQDPDLAIGELERCVGDLGLAGVEIGTHVNAWNLDQEEL
                     FPFFRRARDLGAAVFVHPWDMLGSERMKRYWLPWLVGMPAELALAICSLALGGVLERL
                     PGLRLAFAHGGGSFPFTLGRIDHGYRARPDLCALDSPKPPGAYLDRLVVDSLVHDPEA
                     LRFLVRLLGPERIALGSDYPFPLGEEQPGRLIESLADLPAAAIERMLAGTALEFLGRR
                     REQFA"
BASE COUNT          812 a         1812 c         1908 g          663 t
ORIGIN      
        1 tgccgtcggc cccggcgatc cgcagtggca gggcgatcag ctcgtacacc ccttccggca
       61 cgtgccccag gagcagtccc tcgagaatcg ccatgtcgtg ccgcaggcaa gccttgtgcg
      121 ccggcagatc cttcgagtga cacgggtcga ggctcggcgt gtcgacgccg acgaggcgcg
      181 caccagcatc atgcaggtga tcgatcaacc tcggcgacag cgcggcgaag tcctcgttga
      241 agacggtcgg atcggggaac gttccggtgg cgagcagcac gcgcggcgcc gcgatcgagg
      301 cgccgccgag atcgtccggg ccgagcagcg ccccgcgccg cgccgggacg cgcagcacct
      361 ggcagggacc gaggtagaga tcgagcggcc gggcgtcgat cggcgccgcg ccgcggccgt
      421 agtggctcgg ggcatcggcg tgcgccccga cgtgcacggt cgtgcgcagt gtcgagagcg
      481 tcaggtgcgc ccccgcctcc atgtcgagca gcacctcccg ctccgggggc gtgtcccccg
      541 ggaaaacctt gagggcgcga tcgatcggcg gggtcaggtc gtagatcacg tcggctccgg
      601 caccgttcgg acactcatcc tacagccggt tccggatgag ccagagatcc gggaagatcg
      661 gctggaagag cgtcttccgc agatagtcga ccccgagcga gccgccggtc cccatcttgt
      721 tgccgatggt gcgctgcacc atcatcacgt gccggtagcg ccactcctgc agcccttcgt
      781 cgaaatcggt catcagctcg aggagattgg ccacgtcagg ccgctccttg tacaggcgca
      841 ggagcccctc ctggatctcc gcatccggct ggttcgactc acgaacgtcg cggcggcgca
      901 gcgcctccgg gacggcgacg ccgaggccgg ccaggaagtc gtagaaatga tcgacgagcg
      961 aacgctcttt caggcggcgc tcgaggacgg cgcgcgcggg gtccccctgg ggcagacgct
     1021 cgatcatctc cgggcgcttg ttcccgagca ggaactcgag ctcgcggaac tgcaccgact
     1081 ggaagccgga ggcggcctcg agccggtcgc ggaaggtcag gaacgacatc ggcgtcatcg
     1141 tctcgaggac gtcgatctgc gccaccaggg tcttgagcac catccgcacc cgcttccacg
     1201 tggcgatggc gccgaacagg tcgccggccg tgaagtcgcg ccggatcttc tcgaactcgt
     1261 gcagcagcag ccggaaccac agctcgtaca cctggtggat gacgatgaac agcgtctcgt
     1321 cgtgctccgg ggggtcggag gccggctgct gcagcgccag gagcgcgtcg agcctgaggt
     1381 accgggcgta ggtgaggggg agcggcgcgc cgcccccccg ggatcccgac ttcggcttgg
     1441 tcatcgacgc ctccaatacc acgcgaccgc gacgaccgcc gcggccccga tccccgcggc
     1501 cagcgcctgc agagtcagcg cctgccggcg cgcccggcgc accgtctcgg cgtacgggat
     1561 gcgggtgaac gagatcatca tgtagagcgg catgaacagg ccggggagga ggcggtacag
     1621 ccccttctcc agcccctttc tgagacggaa caggcgcgac gccgtgtggt cgcgcatctc
     1681 gacgaaattg tccaccgaca ggcgcgccag cgcgtcggtg tgctccttgc ggcgtgactc
     1741 gaacaggtcg aacaccgtct cccagtcggg ggcgtgcgcc cggacgcact cgtcgaggac
     1801 gacggcgtcc tcgaaggcgc agttcgcccc ctggccgtgg aacggcacga cggcgtgcgc
     1861 cgcatcgccg agaagcgccg ccttcccgcg cacgtgccac ggccggcagc ggatggtcac
     1921 catcgaaccg accgggttcg tgacgtactg ctcggggagg tccgggaaga gcggcagcgc
     1981 gtcggggaag tgccgctcga agaagcgggt gacggcggac ccgtccgcga gcgcgccgaa
     2041 ggagttcggc cccgcgtgcg gccagaagag ggtgcaggtg aaggagccgt cccggttcgg
     2101 aagcgcgatc atcatgaagc cgccgcgcgg ccagatgtgc agcgcctcgc gctcgaggag
     2161 aaacgatccc ttcgggcccg gcgggatcgt cagctccttg tagccgtggc tcaggaacga
     2221 ctggtcgtag tcgaagcggt cgaggcgctg cagacggcgc cgcaccaccg accaggcgcc
     2281 gtcggtcccg aggacgacgt cgccgcgcac cgtgtcgccc ggcgcatcca tccggccccc
     2341 ggcccctccg tcggggccgg ttccctccgc gggggcgaac gtcgccgacg gggcgtcggg
     2401 atcgatgtcg acacagcgcc ggccgaaggc gaagcggacg ttcttctccc gttcggccgc
     2461 ttcgaggagc agcacgttca gatcggcgcg cgacaccgag tggttcgcgt gcccgcgctc
     2521 ggtgccgtac ggctggaagg cggtgtggcc gcgcaggtcg tggatcatcc ggccgcgcat
     2581 cggcacggcg agggccagga cgcgatcgag gagcccggcc ttctcgaggg cgccgaggcc
     2641 gcgcgccgac agcgccaggt tgatcgagcg tccgccgacg agcggtcccc ggcgcgggtc
     2701 ggggcgcagc tcgaagccgc gcacgtcgtg gccggcccgc ccgaggaaga tcgacgcgag
     2761 cgtgccgccg agccccgcgc cgatctggat gatggtgctc atcgccccgc cgcgcggccg
     2821 cggtcgcggc cgctcacgcg agcgtctgct cgagaacgcg cgcgaagcgc cagatctcgt
     2881 ggaaggtgtt gtagagcggc accggcgcga gacggagcac gtccggctcg cggaaatcca
     2941 gcacgacccc ttcccggtgc agcgcctcca ggagcggctt cgcccgtccg tcccgcacgc
     3001 gcagggagat ctggcagccg cgctcgtcgg gacggcgcgg cgtcagcacc tccacccgcc
     3061 ccggcggcag ccggtcgagc agcgccagca ggtacgaggt catccgcacc gagcgggccc
     3121 gcagggcctc gagccccgcc tcctcgaaga tctgaagcga ggcgaggagc ggcgccagcg
     3181 ccaggatcgg cgggttgccg atctgccagc cgtccgcgcc gggacgaggc acgaactccg
     3241 gcggcatgat gaagcgagtg gccggatccg tcccccacca gccggcgaac cgcggcaggc
     3301 tccggtcgag gccgtggcgc tcgtggacga agcatccagc gaccgccccc ggcccggcgt
     3361 tcaggtactt gtaggagcac cagacggcga aatcgacccg ccactcgtgc agccgcagcg
     3421 gcaggttgcc ggcggcgtgc gccaggtcgg cgccgaagac gcagccgtgc cggtgcgccg
     3481 ccgccgcgag ccgctccaga tcgagcgcct ggccggtcag gtactgcacc cccggcagca
     3541 gcacgacggc gatgctcgag ccgcgctcct ggatcaggcg ctcgagatct tccgtgcgca
     3601 gccccggctc gccggtgcgg tgacgggcga tgaggagcga tgcgtcgggg tcccggccgt
     3661 gatggcggat ctgcgacgcg acggcgtact gatccgaggg gaaggcgtgc tcctcgatca
     3721 ggatggcgtg acgctcgtcg gtcgggcggt agaaggtcgc gagcatcagg tgcaggttca
     3781 cggtgaggcc gttcatcatc acgacctcgc cggggcgggc gccgacgagg cgcgcgccgg
     3841 tctcgcggaa ccgctcgtgg tacgagtacc aggggcggcg cgagcggaag tgcccttcga
     3901 cgccgagcgc ctcccagtcg tccatctctt cgttgacgag ggtgcgcgcc tccttcggca
     3961 tcggcccgag cgaatgaccg cagaagtaca ggagcgggcg gccggaaggt ccagccggca
     4021 gcgcgaaacg ggagcggaag cgggacagcg ggtcctcgag atcgaggcgg tggacctcct
     4081 cctcgtccag agcgggaccg ttcacgagcc caccgtgcat cgccgggccc ggttcacgcg
     4141 aactgctccc gccgccggcc gaggaattcg agcgccgtcc cggcgagcat ccgctcgatc
     4201 gccgcggccg gcaggtcggc gagcgactcg atgagccgtc ccggctgctc ctcgccgagc
     4261 gggaaggggt agtcggagcc gagggcgatc cgctccgggc cgaggaggcg cacgaggaac
     4321 cgcagcgcct ccggatcgtg caccagcgag tccaccacca gccggtcgag gtaggccccc
     4381 ggaggcttcg ggctgtcgag ggcgcagaga tcggggcggg cgcggtagcc gtggtcgatg
     4441 cgcccgaggg tgaacgggaa cgagccgccg ccgtgggcga acgccaggcg gaggcccggc
     4501 agccgctcca gcacccctcc gagcgccagc gagcagatgg cgagcgccag ctcggccggc
     4561 atgccgacga gccaggggag ccaatagcgc ttcatccgct ccgagccgag catgtcccag
     4621 gggtgcacga agacggcggc gccgagatcg cgcgcccgcc ggaagaacgg gaacagctcc
     4681 tcctgatcga ggttccaggc gttgacgtgc gtgccgatct cgacccccgc gaggccgaga
     4741 tccccgacgc agcgctccag ctcgccgatg gcgaggtcgg gatcctgaag gggcacggtg
     4801 ccgaggccga cgaagcgcct cgggtgagcg ccgaccgtct cggcgatgtg gtcgttgagc
     4861 aggcgcgcca cctcgagcgc gtgctccgcc tccgcccagt agcagaacat caccgggaca
     4921 gtcgagagga cctggaccgc gacccctttc cggtcgcact cctccagccg ccgcggcgcg
     4981 tcgaaggtgg aggccgggac gtcgcggaag agcgtcccgt ccaccatcat gcgggccccc
     5041 gcctcggccg gctcaagggt gacgaagccg ccgtagccga agcgctcccg gacgtcgggg
     5101 aggcggggcg gaaggatgtg ggtgtgcaga tcgacccgga acggagtgct cacgcgtccc
     5161 gccccgacgt ccgggagcgc ttcggctacg gcggc
//
LOCUS       DASRIQ010000036         4004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_789987, whole genome shotgun sequence.
ACCESSION   DASRIQ010000036 DASRIQ010000000
VERSION     DASRIQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789987"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFD06_00945"
     CDS             complement(<1..447)
                     /locus_tag="VFD06_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN02130.1"
                     /translation="MIRADGTIALLGDAHLREGDPEVESFVGFLDTLPGQASILAILG
                     DLFAAWIGRADLVRPHHGRVVEALTRLAGRGVRVLYVEGNHDFFLRRLYGSGPFEALA
                     EETLEIAWCGRRLHLAHGDLMNRHDRQYRAWRRLSKSRPFFAAFNVL"
     gene            complement(444..1574)
                     /locus_tag="VFD06_00950"
     CDS             complement(444..1574)
                     /locus_tag="VFD06_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02131.1"
                     /translation="MTGPIAARRRFRRTPILLVVCLAAASLAPSCRGKGGEEQKGGDA
                     ASAAPIDPGQALYNSGKYAEALPLLEQSAAKTRTGTLLYEIGYAKGAVEGVHGTEKTR
                     LWAEAEPLLAQEITSPGGATLDRYYYLTVINFDQREPDKVREYAHQAINQIEMGPDAA
                     KLGGEDWFRLGRLHEFLEEPSESEAAYRRAASAFRERPAANPSYQALALASVAARDLQ
                     ARRFSEAAAQYDQALALAPTLNHVTPYLHGIALLGAGRFNDAATAFMRDRDAETATDA
                     QYAADLARKAEAAGGLEPQDNDGMPIQGMGGDLLDGRIREAAQAFRDLRVKNSWKTGD
                     ALPAELVPAQKRFCALLREKFLQDHLMQEFCLREGIADLVRR"
     gene            complement(1591..2712)
                     /gene="tgt"
                     /locus_tag="VFD06_00955"
     CDS             complement(1591..2712)
                     /gene="tgt"
                     /locus_tag="VFD06_00955"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZN02132.1"
                     /translation="MSRFGFEIVARDQATPARRGRVTTPHGTVETPAFMPVGTAGSVK
                     AMTPEEVAGLGFEMILANTYHLALRPGEEVVHRLGGLHRFMGWSGSILTDSGGFQVLS
                     LSDRRQIGDDGVIFRSHLDGTRLEMTPERSMAIQEALGSDIAMAFDDCPPLPSEPERL
                     RQAVDRTTAWARRSRAAFPDDGRALFGIVQGGDDPALREESAGAIASIGFDAYAIGGV
                     SVGESAALSRSVVARTVPLLPDGKARYLMGMGTPRDLVEMIGLGIDLFDCVLPTRNAR
                     NGSLFTSTGPMQIKRAEFKEDARPIDESCGCDTCRRHSRAYLRHLFMAGEILAFRLNT
                     LHNLHHYADLMRRARAAIDAGRYAAFRDAALAVATSPTG"
     gene            complement(2709..3821)
                     /gene="queA"
                     /locus_tag="VFD06_00960"
     CDS             complement(2709..3821)
                     /gene="queA"
                     /locus_tag="VFD06_00960"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZN02133.1"
                     /translation="MNLDLFDYPLPRERIAQEPAAVRDAARLFLLDRDARAGSGTDDA
                     AGAGEDLLEEIPFQRFPEQVRAGDLVVLNDTRVRPVRLRGRKASGGAVEMLLLDPLED
                     AGTWRALLSTSKGVHAGTRLSIAAGLDAVVTSEPEGGRAVVRLEGPGAENRAGSDPLE
                     AHGVMPLPPYIRRDAAGDARDALDRERYQTVYAREPGAVAAPTAGLHFTPEMLADLPR
                     RGVAVATLSLHVGPGTFLPVRSEAIEDHVVEPERFVLPAATAAAVDGCRDRGGRVVAV
                     GTTVTRVLEARALSGRMEPGGGWCDLYIRPGHRFRAVDALLTNLHLPKSSLLILVAAF
                     AGRERILAAYREAARREARFYSYGDAMFIRSGGRAR"
     gene            complement(3818..>4004)
                     /locus_tag="VFD06_00965"
     CDS             complement(3818..>4004)
                     /locus_tag="VFD06_00965"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HZN02134.1"
                     /translation="LLHLSKADIVREGTRAGAPFHLTWSCYTGTDVACGRCESCRLRL
                     KGFGEAGLPDPLPYARR"
BASE COUNT          555 a         1466 c         1424 g          559 t
ORIGIN      
        1 cagcacgttg aatgcggcga agaagggacg gctcttgctc agccgccgcc aggcgcggta
       61 ctgacggtcg tggcggttca tgagatcacc gtgcgccagg tgcagccgcc gcccgcacca
      121 cgcgatctcc agcgtctcct ccgcgagggc ctcgaacggc cccgagccgt acaggcggcg
      181 caggaaaaaa tcgtggttcc cctcgacgta gaggacccgc accccccgcc ccgccaggcg
      241 cgtcagcgcc tcgaccacgc gcccgtgatg cggacgcacg aggtcggcgc ggccgatcca
      301 cgccgcgaac aggtctccga ggatcgcgag gatcgaggcc tggccgggaa gcgtgtcgag
      361 gaagccgacg aacgactcga cctccggatc gccttcgcgc aggtgggcgt cgcccaggag
      421 ggcgatcgtc ccatcggcgc ggatcaacgg cggacgaggt cggcgatccc ctcgcgcagg
      481 cagaactcct gcatgaggtg atcctgcagg aacttctcgc gcagcagcgc gcaaaagcgt
      541 ttctgcgccg gcaccagctc cgccggcagc gcgtccccgg tcttccagga gttcttcacg
      601 cgcaggtcgc ggaaggcttg ggccgcctcg cggatgcgcc cgtcgagcag gtcgcccccc
      661 atcccctgga tcggcatgcc gtcgttatct tgaggttcga gcccgcccgc cgcctccgcc
      721 ttgcgggcga gatcggcggc gtactgggcg tcggtggcgg tctcggcgtc gcgatcgcgc
      781 atgaaggcgg tcgccgcgtc gttgaagcgg cccgcgccga gaagtgcgat gccgtgaagg
      841 tagggcgtca cgtggttgag cgtcggagcg agcgccagcg cctggtcgta ctgcgccgcg
      901 gcctccgaga agcggcgcgc ctgcaggtcg cgcgcggcga ccgacgcgag cgccagggcc
      961 tggtagctcg ggttcgccgc cggccgctcc cggaacgccg aggccgctcg ccggtacgcc
     1021 gcctccgact ccgacggctc ctccaggaac tcgtgcaggc gcccgagccg gaaccagtcc
     1081 tcgccgccga gcttcgccgc gtccggtccc atctcgatct ggttgatcgc ctggtgcgcg
     1141 tactcccgca ccttgtccgg ctcccgctga tcgaagttga tgacggtcag gtagtagtag
     1201 cgatcgagcg tcgcaccgcc gggggaggtg atctcctgcg ccagcagcgg ctccgcctcc
     1261 gcccacaggc gcgtcttctc ggtgccgtgc accccttcga ccgcgccttt ggcgtagccg
     1321 atctcgtaca ggagggtgcc ggtgcgcgtc ttcgcagccg actgctcgag gagcggcagc
     1381 gcctcggcgt acttgccgct gttgtagagg gcctgcccgg gatcgatcgg ggccgcgctc
     1441 gcggcgtcgc cacctttctg ctcctcccca cccttgccgc ggcacgaggg ggcgagcgag
     1501 gccgccgcca gacacacgac gagcaggatc ggggtgcgcc ggaatcggcg acgcgctgcg
     1561 atcgggcccg tcatgggagc gcggatggta tcacccggtc ggagacgtcg caaccgcgag
     1621 cgccgcgtcg cggaacgccg cgtagcgtcc ggcgtcgatg gcggcgcggg cgcgccgcat
     1681 caggtccgcg tagtgatgga ggttgtgcag ggtgttgagg cggaaggcga gaatctcgcc
     1741 cgccatgaag aggtgccgca ggtaggcgcg cgagtgccga cggcacgtgt cgcagccgca
     1801 cgattcgtcg atcggacgcg cgtcctcctt gaactcggca cgtttgatct gcatcggccc
     1861 cgtggaggtg aacaggctcc cgttgcgcgc gttgcgggtc ggcaggacgc agtcgaagag
     1921 gtcgatcccg agcccgatca tctcgacgag atcccgcggc gtccccattc ccatgaggta
     1981 gcgcgccttc ccgtcgggca ggagcggcac ggtccgcgcc acgacgctgc ggctcaaggc
     2041 ggcgctctcg ccgaccgaga ctccaccgat ggcgtaggcg tcgaatccga tcgaggcgat
     2101 cgccccggcg ctctcctccc gcagcgccgg gtcgtcccct ccctgcacga tgccgaacag
     2161 ggcgcgtcca tcgtccggga aggcggcgcg gcttcgcctc gcccacgcgg tggtccgatc
     2221 gaccgcctgg cgcagccgct ccggctccga cgggagcggc gggcagtcgt cgaacgccat
     2281 ggcgatgtcg ctgccgagcg cctcctggat cgccatgctg cgctcggggg tcatctcgag
     2341 gcgcgtgccg tcgaggtggc tgcggaagat gacgccgtcg tcgccgatct gccgccggtc
     2401 cgacaggctc agcacctgga agccgccgct atcggtgaga atcgatccgc tccatcccat
     2461 gaagcggtgc agcccgccca gccggtggac gacctcctcc cccggccgca gcgccaggtg
     2521 gtaggtgttt gccaggatca tctcgaagcc gagtcccgcg acctcctccg gcgtcatcgc
     2581 cttgaccgat ccggcggtcc cgaccggcat gaaggccggt gtctcgaccg tgccgtgcgg
     2641 cgtcgtgacg cggccccgcc gcgccggcgt cgcctggtcg cgcgccacga tctcgaatcc
     2701 gaagcggctc accgcgcccg gcccccggag cggatgaaca tcgcgtcgcc gtaggagtag
     2761 aagcgcgcct ccctgcgcgc cgcctcgcga tacgcggcga ggatccgctc ccggcccgcg
     2821 aaggcggcca ccaggatgag cagcgacgat ttcggcaggt gcaggttcgt caggagcgcg
     2881 tcgacggcgc ggaagcggtg ccccgggcgg atgtacaggt cgcaccagcc gcctcccggc
     2941 tccatccgcc cggagagggc gcgcgcctcg aggacgcgcg tcacggtcgt gccgaccgcg
     3001 acgacgcggc cgccccggtc ccggcagcca tcgaccgccg ccgccgtcgc cgcggggaga
     3061 acgaaacgct ccggctcgac gacgtgatcc tcgatggcct cggagcgcac cggcaggaag
     3121 gtgccgggac cgacgtgcag cgacagggtc gcgaccgcca ccccccggcg tggcaggtcg
     3181 gccagcatct ccggcgtgaa gtgcagcccc gcggtggggg cggccaccgc ccccggctcg
     3241 cgcgcgtaga cggtctggta gcgctcccgg tcgagggcgt cgcgcgcgtc gccggccgcg
     3301 tcgcgccgga tgtagggggg aagtggcatc acaccgtggg cctcgagagg atcgctgccg
     3361 gcgcggtttt cggcgccggg tccctccagc cgtaccaccg cccgtccccc ttccggctcc
     3421 gaagtgacga ccgcgtcgag ccccgccgcg atgctcaggc gggtgccggc atggactcct
     3481 ttcgaggtcg acaggagcgc ccgccaggtg ccggcgtcct cgagcggatc aaggaggagc
     3541 atctccacgg ctccgccgga cgccttccgc ccgcgcaggc gcaccggccg cacccgcgtg
     3601 tcgttgagga cgacgaggtc cccggcccgc acctgctccg gaaagcgctg gaacgggatc
     3661 tcctcgagga ggtcctcgcc cgccccggcc gcgtcgtccg tcccggaccc ggcgcgcgcg
     3721 tctcggtcca ggaggaacag gcgcgccgcg tcgcgcaccg cggccggctc ctgcgcgatc
     3781 ctctctcgag gcagcgggta gtcgaacagg tcgaggttca ccgcctcgcg tacggcagcg
     3841 gatcgggaag ccccgcctcg ccgaacccct tgagacgcag gcggcacgat tcgcagcggc
     3901 cgcaggcgac gtccgtcccg gtgtagcacg accaggtgag atggaacggc gccccggcgc
     3961 gcgttccttc gcgcacgatg tccgccttgc tcaggtgcag gagc
//
LOCUS       DASRIQ010000037         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3136377, whole genome shotgun sequence.
ACCESSION   DASRIQ010000037 DASRIQ010000000
VERSION     DASRIQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3136377"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFD06_00970"
     CDS             complement(<1..357)
                     /locus_tag="VFD06_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZN02135.1"
                     /translation="MEGTFAERLAAVRERIAKAALQAGRSVDSVTLIGVSKTVPAARV
                     KEAVAAGLLELGENRVQEAREKIPAVGPGPRWHLVGHLQGNKAWQAARLFGAVHSIDS
                     VDLLGRLDRAAAAAGRR"
     gene            complement(455..937)
                     /locus_tag="VFD06_00975"
     CDS             complement(455..937)
                     /locus_tag="VFD06_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HZN02136.1"
                     /translation="MSRAHLRANIDGGARGNPGPAGFGVHVVSAGDGALVAGLYGYLG
                     VATNNIAEYAALLALLEHAGAATPALLEVRSDSLLLVRQMRGEFKVKDPKLQILHAAA
                     RRLAARLPKVTYAHVPREENRDADALANRAMDERSSNTPIPAVLSSLPPIATHPKLND
                     "
     gene            complement(934..1749)
                     /locus_tag="VFD06_00980"
     CDS             complement(934..1749)
                     /locus_tag="VFD06_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HZN02137.1"
                     /translation="MQQDLANLIRLQDLMLRIDAMNEQIAAIPAEVARLEKDLLEAQK
                     GVEQEKSALAELQKERRRLEGELMGVEAKIQKYQAQLMDVKTNKEYQAMQHEIEGCKA
                     ERASLDEKILLEMEEQEKGNTASRALEDRLKEMRRRTEDGKKALADKGAALKRDRDVI
                     DADRQALQKEVSPVFLDPFLKTARQRRGIGLVGVRDGLCGGCHVRVLPKLIQEVRRST
                     HLIVCETCKRFLYVVDDVVASGPARSPGAPEGASEPARPETGGDGESPDPRVS"
     gene            1790..>2139
                     /locus_tag="VFD06_00985"
     CDS             1790..>2139
                     /locus_tag="VFD06_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HZN02138.1"
                     /translation="MRRAWPLHSRILVGMIAGALLGLVADALWGGAPWLAGFITWVTE
                     PVGRIFLRLIFMVVIPLVVSALVLGVAEIGDVRRLGRVGLRTLLYTVVLSAVSVLLGV
                     GLANLIRPGEGMSP"
BASE COUNT          275 a          753 c          747 g          364 t
ORIGIN      
        1 ccggcgcccg gccgccgccg cggcgcgatc cagccggccg agcagatcga ccgaatcgat
       61 cgagtgcacc gcgccgaaca gccgcgccgc ctgccacgcc ttgttcccct ggaggtgccc
      121 cacgaggtgc cagcggggcc cggggccgac ggccggaatc ttctcgcgcg cctcctgcac
      181 ccggttctcc cccagctcga ggagcccggc ggcgaccgcc tccttcacgc gcgccgcggg
      241 caccgtcttg ctgacaccga tgagggtgac ggagtccacc gaccgaccgg cctgcagggc
      301 cgccttcgcg atgcgctcgc ggacggccgc cagacgctcc gcgaaggtcc cctccatggg
      361 cgatcaatat aggtagggcg tccgaagtgt caaacccttg gcgatcggcg gcacaggcca
      421 cgaaggcccg cggggaccct cggcccgcgg gaggtcagtc gttgagtttg ggatgcgttg
      481 cgatcggggg gaggctggag agaacggcgg ggatgggggt gttcgaggag cgttcgtcca
      541 tggcgcggtt ggcgagggcg tcggcgtcgc ggttctcctc gcgggggacg tgcgcataag
      601 tgaccttcgg gaggcgcgcc gccaggcggc gggcggcggc gtgaaggatc tgaagcttcg
      661 ggtccttcac cttgaactcg ccgcgcatct gccggacgag caggagcgag tcggatcgga
      721 cttcgagaag agccggggtg gcggcgccgg cgtgctccag gagcgccagc agggcggcgt
      781 actcggcgat gttgttggtg gcgacgccca ggtagccgta gaggccggcg acgagggcgc
      841 catccccggc cgagacgacg tgcactccga atccggcggg gccgggattg ccgcgcgccc
      901 ctccgtcgat gttggcgcgg aggtgcgccc ggctcacgac acgcgggggt cgggagattc
      961 cccgtcgccc cccgtctcgg ggcgggccgg ctcggaagcc ccttccggcg cgcccgggct
     1021 ccgggccggt cccgaggcca ccacatcatc gacgacatag aggaagcgct tgcaggtctc
     1081 gcagacgatg aggtgcgtcg agcgccgcac ttcctggatc agcttcggca gcacccggac
     1141 gtggcagccg ccgcacagcc cgtcacggac accgacgaga ccgatgccgc gcctctggcg
     1201 cgccgtcttg agaaaggggt cgaggaagac cggggagact tccttctgca gcgcctgtcg
     1261 atcggcgtcg atgacgtccc ggtcgcgctt gagcgccgcg cccttgtcgg cgagcgcctt
     1321 cttcccgtcc tcggtccggc ggcgcatctc cttcaggcga tcctcgaggg cgcgggaggc
     1381 ggtattgccc ttctcctgct cctccatctc cagcaggatt ttctcgtcca gggaggcgcg
     1441 ctcggctttg cagccctcga tctcgtgctg catcgcctgg tattccttgt tggtcttcac
     1501 gtccatgagc tgcgcctggt acttctggat cttcgcttcc acccccatga gctccccttc
     1561 caggcggcga cgctccttct gcagctcggc gagagcggac ttctcctgct ccaccccctt
     1621 ctgggcctcg aggagatcct tctccaggcg cgcgacctca gccgggatcg cggcgatctg
     1681 ctcgttcatc gcgtcgatcc gcagcatgag gtcctggagt cggatgaggt tcgcgaggtc
     1741 ctgctgcatg aagaagcgaa ttgtaacacc tatgctaggg tacccggccg tgcgccgcgc
     1801 gtggcctctc cactcccgca tcctcgtcgg catgatcgcg ggggcgctcc tgggcctcgt
     1861 cgccgacgcc ctctggggcg gcgccccgtg gctcgccggc ttcatcacct gggtgaccga
     1921 gccggtcggg cgcatcttcc tgcggctcat cttcatggtg gtgatcccgc tggtcgtctc
     1981 cgccctcgtc ctcggtgtgg cggagatcgg cgacgtgcgc cggctgggcc gggtcggcct
     2041 gaggaccctc ctctacaccg tcgtgctgag cgccgtctcg gtcctgctcg gcgtcggtct
     2101 cgccaacctc atccgccccg gcgaagggat gtccccgga
//
LOCUS       DASRIQ010000038         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_791064, whole genome shotgun sequence.
ACCESSION   DASRIQ010000038 DASRIQ010000000
VERSION     DASRIQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791064"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..693)
                     /locus_tag="VFD06_00990"
     CDS             complement(52..693)
                     /locus_tag="VFD06_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02139.1"
                     /translation="MRLPVRPLAVLSLVLLAGCASNPPARRVWVGPRIDLKPYETIGM
                     VEFTSSSKGKLAALATRRFEEAARRDQDMLRMVDVGTRNQALASVDGDAWDAASFRAV
                     GRKHGVKTMFEGTLTVSKVHPTVALSAFFKSGQVNANVNAALEVRMIETETGASIWSR
                     SAATTRTLGQVSVFGGKEFVFDAADPEAAYGALVDDLVSQVAQDLQGSWQLQR"
     gene            898..>2471
                     /locus_tag="VFD06_00995"
     CDS             898..>2471
                     /locus_tag="VFD06_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02140.1"
                     /translation="MKARLTIPAAALLIAGVAPACTSSVTTAPDLRPELEVHGRVESL
                     EVNPAGSLWFGTATGRLYVSRDWNLSWSEVPSPAGDSDRDMLSSDVISRVRFFDAQRG
                     IVTGYLGTSQDFVYRTTDGGTTWSTVRFPAPGSPWVYDAQTTDAGLAWLVGSDGGLYF
                     SDDFAASWSALSAPFDDVSRSLSVHFVSRSLGVVGSLGGSLKVTRDGGRSWRRMKSPA
                     ETGVTECKDARVDLARILGERVVIRQCGAVFSRAMSKEGSWLPLRVADRPLVAFAING
                     DALVAVASDFEIVDCARDLQTCRPTGARLDAAPLDITTGAARVVFIDTNLKVSVLEKQ
                     GLRSSRMLGTPTDEDPPLSARDRGRDGAFWGVSRFFLYRSRDEGRTWERQAELPREVD
                     GLAIQDSGDVLIWSRHGLVTRWSPEAGRLLPVPALEGLDIVGLFRRGSLWLAYGGMQD
                     ETTRRIEVARTYFSGQFHGTADHGYVAASLDGGASWRVVDEWKDGGVQSLFLGEDGTL
                     TLLSWLCAVRRGRLSV"
BASE COUNT          345 a          848 c          843 g          435 t
ORIGIN      
        1 cttccgcccg cgcggccggt cgcagggccg gcctgccggg cgaagtcgtt cctaccgctg
       61 tagctgccag gacccctgga gatcctgcgc cacctgggag acgaggtcgt cgaccagggc
      121 cccgtaagcg gcctccgggt cggcagcgtc gaacacgaac tccttcccgc cgaacacgct
      181 gacctggccg agggtgcggg tggtggcggc ggagcggctc cagatcgacg ccccggtctc
      241 cgtctcgatc atccgcacct cgagcgccgc gttgacgttg gcgttcacct ggccggactt
      301 gaagaaggcc gacaaggcga cggtcggatg caccttggag acggtcaggg tcccctcgaa
      361 catcgtcttc acgccgtgct tgcgtccgac ggcgcggaag gacgcggcat cccaggcgtc
      421 gccgtccacc gacgcgagcg cctggttccg ggtgccgacg tccaccatgc gcagcatgtc
      481 ctggtcgcgg cgcgccgcct cctcgaaccg gcgggtcgcg agggccgcga gctttccttt
      541 cgaactggac gtgaactcca ccatgccgat ggtctcgtac ggcttcagat cgatgcgcgg
      601 accgacccag acgcggcgcg cgggcgggtt gctggcgcag ccggcgagaa gcacgagcga
      661 caggacggcg agcggacgga cgggcagacg catcgttccc cctccttcga cccggacaac
      721 ctaggtccct ccgcgcgcgg tcgcaatgcg atgacgatct cgtcgccggg ccggccccgg
      781 tccaggctcg ctccaccgtc gatcttctga caccatgctg cgatggtgcc gcgggaactc
      841 cgtccctccc gtttgccccc gtgtgtcgtg cgcgtacctt cccaacgagg tgaccgcatg
      901 aaggcgaggc tcacgatccc ggccgcggct cttctgatcg ccggggtcgc gccggcgtgc
      961 acgtcctcgg tgacgacggc acccgatctc cgccctgagc tcgaggtcca cgggcgggtc
     1021 gagtcgctcg aagtgaaccc tgcaggctct ctctggttcg ggacggcgac cggccgcctc
     1081 tacgtgagcc gggactggaa tctctcctgg agcgaggtgc cgagtcccgc cggggattcg
     1141 gaccgcgaca tgctctcttc cgatgtcatc agtcgagtgc gcttcttcga cgcgcagcgc
     1201 ggcatcgtca ccggatacct ggggacctca caggatttcg tctaccgcac gaccgatggc
     1261 ggaacgacct ggtcgaccgt ccgatttccc gcaccaggca gcccctgggt ttacgacgcc
     1321 cagaccaccg atgcagggct cgcctggctc gtcggttccg acggcgggct ctatttctcg
     1381 gacgacttcg cggcttcgtg gtcggccttg tccgccccgt tcgatgacgt ctcgcgatcc
     1441 ctcagcgtcc atttcgtctc gcgttcgctc ggagtcgtcg ggtccctcgg ggggtcgctg
     1501 aaggtcacgc gcgacggcgg gcgatcctgg cgtcgaatga agtcacccgc cgagaccggc
     1561 gtcaccgagt gcaaagacgc acgcgtcgat ctggcgcgca tcctcgggga aagagtcgtc
     1621 atccggcaat gcggcgccgt gttctctcgc gccatgtcga aggagggatc ctggctcccg
     1681 ctccgggtcg ccgatcggcc gctggtcgcc ttcgccatca acggggatgc tctggtcgcg
     1741 gtcgcatcgg atttcgagat cgtcgattgc gctcgcgatc ttcagacctg ccgtccgacc
     1801 ggagctcgtc ttgacgccgc tcccctggac atcacgaccg gtgcggcgag agtcgtcttc
     1861 atcgacacga acctgaaggt ctccgtcctc gaaaagcaag ggctccggtc gtcgcgcatg
     1921 ctggggacgc cgaccgacga agatccgccg ctctcggcgc gtgatcgcgg acgcgacggg
     1981 gcgttctggg gagtttcgag attctttctg tatcggtccc gcgacgaggg gcggacctgg
     2041 gagaggcagg ccgagctgcc gcgcgaggtg gacgggttgg cgattcagga ctcgggtgat
     2101 gtcctcatct ggagccggca cggtcttgtg acccgctgga gcccggaggc cggccggctg
     2161 cttccggtcc cggcgctcga gggcctggac atcgtcggtc tcttccggcg cggatcgctc
     2221 tggctggcct acggcggaat gcaggatgag acgacgcggc ggatcgaggt cgcgcggacc
     2281 tatttctccg gacagttcca cggcaccgcg gaccatggct atgtcgccgc ctcgctcgac
     2341 ggcggtgcga gctggcgggt cgtcgatgag tggaaggatg gcggggtgca atcgctcttc
     2401 ctcggcgagg acggcacgct gaccctcctg tcctggctct gcgcggtccg ccggggccgc
     2461 ctttcggtcg a
//
LOCUS       DASRIQ010000039         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3400269, whole genome shotgun sequence.
ACCESSION   DASRIQ010000039 DASRIQ010000000
VERSION     DASRIQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3400269"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFD06_01000"
     CDS             <1..296
                     /locus_tag="VFD06_01000"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZN02141.1"
                     /translation="GGLEGGMSNGEEIRVRALVKPLSTLPRALPSVDLVTKEPFTAQV
                     ERTDVTAIAAAGVVGEAMTAYVLARGFREKFGGDSVSEMARNHAAYLEALREF"
     gene            262..702
                     /locus_tag="VFD06_01005"
     CDS             262..702
                     /locus_tag="VFD06_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZN02142.1"
                     /translation="MPPTSKRSGSSRLSGPPSRAGAHPGRGAFPAIYAVVARIPRGRI
                     ATYGQVARMAGMPGAARTVGWAMAALPNRGRIAGRAIPWHRVLNAQGGVSPRPSETPG
                     RPDGQAARLRREGLETGKNGRFDLERYGWDGTGRGSPKRSGRSG"
     gene            complement(645..>2146)
                     /locus_tag="VFD06_01010"
     CDS             complement(645..>2146)
                     /locus_tag="VFD06_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN02143.1"
                     /translation="FDRNPVGRLMTRVTTDVESLADLFASGAVTLLGDVVKLVAIVAI
                     LAWLDARLALVTCTIVPVLFVLSILFRGRIRQAYRDVRRRIARINAFLQEAVSGMMLT
                     QLFRREEQDRREFQAINDDHREAELRSVVYESSFSAVVELVGTLATAAILWYGGARVL
                     SSALTFGTLVAFLEYTSRFFQPIRELSGFYAVLQAAMASLERLFDLLDTESSVVDGPR
                     PGASGAVVVSASDRTRPGAGRIEFDRIGFRYLPEEPVLTDVSFMVEPGQTVALVGATG
                     AGKTTVVKLLIRLYDPTSGAIRLDGIDLRDRPVHEVRRQCGLVMQDHFLIAGTVAENI
                     AFGDLGLSRARIEAAARLVHADRFVRGLPKGFDEPLRERGGNLSVGQKQLLSLARAVA
                     SDPPILILDEATSNIDSETEALIQDALAQVMRGRTSLVIAHRLSTILAADRILVFHHG
                     RIVEEGRHRELLARRGVYARLYELQFGARTAQPERPERFGEPLPVPSQP"
BASE COUNT          335 a          741 c          796 g          274 t
ORIGIN      
        1 ccggcggcct cgaagggggc atgtcgaacg gcgaggagat ccgcgtacgc gcgctggtca
       61 agccgctctc gacgctgccg cgggcactgc cgagcgtcga cctcgtcacc aaggagccgt
      121 tcacggcgca ggtggagcgg accgacgtca ccgccatcgc cgcagcgggt gtggtggggg
      181 aggcgatgac ggcgtatgtc ctcgcccgcg gcttccgcga gaagttcggc ggcgactccg
      241 tctccgagat ggcccgcaac catgccgcct acctcgaagc gctccgggag ttctagactc
      301 tccggaccgc cgtcccgcgc cggcgcccat ccggggcgcg gggcgtttcc ggccatctac
      361 gccgtggtgg cgcgtatccc gcgcggccgc atcgcgacct acggtcaggt ggcgcgcatg
      421 gcggggatgc cgggggcggc ccgcaccgtc gggtgggcga tggccgccct tcccaaccgc
      481 ggtcgaatcg ccggacgagc gatcccctgg catcgcgtcc tgaacgccca gggaggggtg
      541 agcccacgac cctccgagac gccggggcgt cccgatgggc aggcggcgcg cctgcgccgc
      601 gaggggctcg agaccgggaa gaacggacgg ttcgatctcg agcgctacgg ctgggacggc
      661 accggcaggg gctccccgaa acgctccggc cgctccggct gagccgtccg cgctccgaac
      721 tgcagctcgt acagccgcgc gtacacgccg cgtcgggcca gaagctcccg gtgccgccct
      781 tcctcgacga tccgcccgtg gtggaagacg aggatccggt cggccgccag gatggtcgac
      841 agccggtggg cgatgacgag ggaggtccga ccgcgcatca cctgcgccag ggcgtcctgg
      901 atgagggctt cggtctcgct gtcgatgttc gaggtcgctt cgtcgaggat gaggatcggc
      961 gggtcggacg cgacggcccg cgccagcgac aggagctgct tctggccgac cgagaggttc
     1021 ccgccgcgct cccggagcgg ctcgtcgaac cccttcggca ggccgcgcac gaagcgatcg
     1081 gcgtgcacga gccgcgccgc cgcctcgatc cgggcgcgcg aaaggcccag gtccccgaag
     1141 gcgatgttct ccgccaccgt gccggcgatg aggaagtgat cctgcatgac gagaccgcac
     1201 tgccgccgca cctcgtgcac ggggcgatcg cgcaggtcga tgccgtcgag ccggatggcg
     1261 cccgacgtcg gatcgtagag gcggatgagg agcttcacga ccgtcgtctt accggcgccg
     1321 gtggcgccga cgagcgccac ggtctgtccc ggctccacca tgaaggagac gtcggtcagg
     1381 accggttcct cggggaggta gcggaagccg atccggtcga actcgatccg ccccgcgccc
     1441 gggcgcgtgc ggtcggaggc gctcaccacc accgcgcccg atgccccggg gcgcggtccg
     1501 tcgacgacgg aggactcggt gtcgagcagg tcgaagagac gctcgagcga ggccatggcc
     1561 gcctggagca cggcgtagaa ccccgagagc tcccggatcg gctggaagaa gcgggaggtg
     1621 tactcgagga acgcgacgag cgtcccgaag gtcagcgcgg acgacaggac ccgggcgccg
     1681 ccgtaccaga ggatcgcggc ggtggccagg gtgccgacga gctcgacgac cgccgagaag
     1741 gacgactcgt agacgaccga gcgcagctcg gcctcgcggt ggtcgtcgtt gatcgcctgg
     1801 aactcgcgtc ggtcctgctc ctcgcgacgg aagagctggg tcagcatcat gccgctcacc
     1861 gcctcctgca ggaaggcgtt gatgcgggcg atgcgccgcc gcacgtcgcg gtacgcctgc
     1921 cggatgcgcc cgcggaacag gatggagagg acgaagagca ccgggacgat ggtgcaggtg
     1981 acgagcgcca ggcgcgcgtc gagccacgcg aggatcgcca cgatcgcgac cagcttgacc
     2041 acgtccccga ggagcgtcac cgccccgctg gcgaagagat ccgcgagcga ttcgacgtcg
     2101 gtcgtcacgc gcgtcatcaa ccggccgacc gggttgcgat cgaaga
//
LOCUS       DASRIQ010000040         5906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2880662, whole genome shotgun sequence.
ACCESSION   DASRIQ010000040 DASRIQ010000000
VERSION     DASRIQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880662"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFD06_01015"
     CDS             <1..358
                     /locus_tag="VFD06_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZN02144.1"
                     /translation="TFCRHEIAAYFERTGRERPSLPLAEEVPEIRAALESLSAAAGND
                     PAAALRRREIARLVHVTLDHLPERYADVLEWKYIEGIAVQEIAARLGSTTKAAESLLT
                     RAREAFRDGFATLNAT"
     gene            361..1320
                     /locus_tag="VFD06_01020"
     CDS             361..1320
                     /locus_tag="VFD06_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HZN02145.1"
                     /translation="MESKDPIALLLRLTGPRPAVPADRAARVKAAVRADWRAAVERGA
                     RRRTRWRLAALAAILTVAVAVPLMRGWRPGSGPAPEAARIELGSSAAERGTSVPAGSV
                     LATGDDERLALRMASGPSVRLDTATRARIDSGGAVELLAGAIYVDSGDRSPGGGSAAI
                     EIRTPLGPVRDLGTQFEVRLVTAGVRLRVREGTVSLERPAGPLEVRAGRELLAGADGS
                     AREDPLADPAGAWAWIGAITPMMAIDGRSLPEFLAWFGRERGLEIRYADRDLAAAAAA
                     IRLNGSIDGMTLDESLASVLAISRLEHRIEGTSLFIERPAGAQ"
     gene            1325..3838
                     /locus_tag="VFD06_01025"
     CDS             1325..3838
                     /locus_tag="VFD06_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN02146.1"
                     /translation="MRTGRIHLLAWGFLALFGGALAGADDAAPRWRGRRLADALQELR
                     GMGLDLVFSSATVTADRIVTIEPAATTPRGILDAILTPLGLEAQDARGGAILIVPASK
                     GSKDAVIRGRVVSATSGQPVAGAVIRIAGTAATAVSRPDGSFRLNVPAGTRALTAAAH
                     GFIERTVDAIAGADGRIREILVPLPPRPGYVEEIVVTPSRRSLAREEPAPRLTLDHQD
                     TELVPAIGGDAVRAVESLPGMAAADNSAAFNLRGSEARDVSLILDGLELYDPFHLAAL
                     QAPWSVIDGALVDRIDVLGGGFTADRGDRRGGFVEMSTSLPDGPERMHAAVGSLQSGF
                     SYGAPTGSGSLLVAGRAWYPEALGSTIEIGEPGLDPRFADLYVKSSFVVSPQTALSLH
                     ALAGSDRLEFRESAGNEQVALVEKSTHLWVRLLQAPTPKLLLETVASGGRLTRSRVGA
                     AEPEDEVISVDDDRTIDFFGVRSDVTWAVREDRLLKFGLQARPMSSDYRYATGPAGDP
                     AAGTSLRLDPQGTSFAAYAAWRTGLSDRVVLEAGLRWDRQTYTDDDEMSPRLNAVWRA
                     SDRTTLRLGLGRFTQSQRLHELDIEDGESMFHPAERSRQADLTLEHRTDAGLRFRFDG
                     YYCAITRPQPRSENLFNPIELFPEVSPDRVRIAPDGARLRGAELFLGGDPQHRFVWWG
                     SYAWSSAEDRVDGATAPRSWDQTHAVRLAAAWRPDARWTIALLGTARSGWPTTPVTAE
                     SELQPDGSTEIVATPGRRNSDRFPYYARLDLKGSVVLPAARGRLRLEIDVTNITDRAN
                     VCCVDEFVFDTLPDGSIDTRTDLDHWLGATTAVNVEWSF"
     gene            complement(3881..5761)
                     /locus_tag="VFD06_01030"
     CDS             complement(3881..5761)
                     /locus_tag="VFD06_01030"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HZN02147.1"
                     /translation="MTTRMKTEGAPKGAGQMDLFLAAPHPAAAARRPERPEGRGSEVA
                     AKRGEILAAKPGRGAKPAGKPKGNAGSRRGARPTPAAPRTEAEPEAPPAPEPAAAASG
                     GGETPPARRRETAESIGKRQREISVAEFFQKNRHLLGFDNPQKALLTATKEAVDNSLD
                     ACEEAGILPALEVEIEEKAEDRFRLAITDNGPGIVRNQIPKVFGKLLYGSKFHTMRQS
                     RGQQGIGISAAGMYSQLTTGQPVRITSRTGKKKPAHYFEISIDTTRNEPKIIVDREVE
                     WDRDHGTKVEMELAGLYKKGRRSVDDYVIQTALANPHADVHYTPPKGDPFHRARLTKE
                     LPREPLVIKPHPYGVELGILIAMLQDSKSRTVSTALQSDFARVSDRVALEICKEAKVN
                     PNARPRSLPTADIESLFRAIPKVKIMAPPTNVIVPIGEELILEGLKQTVKADFYASAT
                     RPPAVYRGNPFLIEVGLAYGGDLPGEEPVDLWRFANRVPLQYLQSACAITRAAVTTDW
                     RNYAMQQSRGALPTGPMILFVHMASVWVPFTSESKEAVAGYPEILRELRLGLMEVGRR
                     LGGWLRHRRRLAEEMKKQSYIKSYIPHIGIALREILGLTKQEEAKVVSTLTETLEKSR
                     TL"
BASE COUNT          746 a         2144 c         2110 g          906 t
ORIGIN      
        1 cactttctgc cggcacgaga tcgcggcgta tttcgagcgc accggccgcg aacggccgtc
       61 ccttcccctc gccgaggagg tgccggagat ccgggcggcg ctcgagtcgc tctcggccgc
      121 ggcggggaac gacccggcgg cggccttgcg gcgccgcgag atcgcgcggc tggtgcacgt
      181 gacgctcgat cacctgccgg agcgctacgc cgacgtcctc gagtggaagt acatcgaggg
      241 gatcgcggtg caggagatcg cggcccgcct gggatcgacc accaaggcgg ccgaatcgct
      301 cctgacccgc gcccgcgaag ccttccgcga cggcttcgcg accctgaacg cgacatgacg
      361 atggagagca aagacccgat cgccctgctc ctgcgcctga ccgggccccg gcccgccgtc
      421 ccggccgacc gggcggcccg ggtcaaggcg gcggtccgcg ccgactggcg cgccgcggtc
      481 gaacgcggcg cgcggcggcg gacccgctgg aggctcgcgg cgctggcggc gatcctcacc
      541 gtcgccgtgg ccgtccccct gatgcgcggc tggcgccccg gctcggggcc ggcccccgag
      601 gccgcgcgga tcgagctcgg ctccagcgcc gctgagcgcg gcacgagcgt tccggccggc
      661 tccgtgctcg cgaccggaga cgacgagcgc ctggcgctcc ggatggcctc ggggccgtcg
      721 gtccggctgg acaccgccac tcgggcgcgc atcgactccg gcggcgcggt cgagctcctg
      781 gcgggagcga tctacgtcga ttcgggtgac cggtcccccg ggggcgggag cgcggcgatc
      841 gagatccgca cgcctctcgg cccggtgcgg gacctcggga cgcagttcga ggtccgcctc
      901 gtcacggcgg gcgtgcggtt gcgcgtgcgc gaagggacgg tgtcgctcga gcgtcccgcc
      961 ggccccctcg aggtgcgggc gggacgcgag ctcctggccg gcgccgacgg gagcgcacgc
     1021 gaggatccgc tcgccgatcc cgccggggcc tgggcctgga tcggggcgat cactccgatg
     1081 atggcgatcg atggacggtc gctgccggaa ttcctggcct ggttcggccg cgagcgcggc
     1141 ctcgagatcc gctacgcgga ccgcgatctc gccgcggcgg ccgcggcgat ccggctgaac
     1201 ggctccatcg acggcatgac gctcgacgag agcctcgcgt cggtcctggc gatctcccgc
     1261 ctggagcatc ggatcgaggg gacgtcgctg ttcatcgagc gcccggccgg cgcacaatga
     1321 cgccatgcgg acaggccgga ttcacctttt ggcgtggggg tttctcgcgc tcttcggcgg
     1381 cgcgctcgct ggcgcggacg acgccgcgcc gcgctggcgc ggccggcgtc tcgccgacgc
     1441 gctccaggag ctgcgcggga tggggctcga cctcgtcttc agcagcgcga cggtcaccgc
     1501 cgaccgcatc gtcaccatcg agccggccgc gacgacaccg cgtgggatcc tcgacgcgat
     1561 cctgacgccg ctcgggctcg aagcccagga cgcgcgcggc ggggcgatcc tgatcgtgcc
     1621 ggcctcgaag ggatcgaagg acgcggtgat ccggggccgg gtggtctcgg cgacgagcgg
     1681 ccagccggtg gcgggcgcgg tcatccggat cgccgggacc gcggccaccg cggtgagccg
     1741 ccccgatgga tcgttccgcc tcaacgtgcc ggcgggcacc cgcgccctca ccgccgcggc
     1801 ccacggattc atcgaacgga cggtcgatgc gatcgccggc gccgacggcc ggattcggga
     1861 gatcctcgtt ccgctcccgc cgcgcccggg atacgtcgag gagatcgtgg tcacgccgag
     1921 ccgccgatcc ctggcgcggg aggagccggc cccccgcctc accctcgacc accaggacac
     1981 cgagctcgtg cctgcgatcg gcggcgacgc cgtccgtgcg gtcgagtcgc tcccgggaat
     2041 ggccgcggcc gacaattccg ccgccttcaa cctgcgtggc tccgaggcgc gcgacgtgtc
     2101 gctcatcctg gacggcctgg agctgtacga tccgttccac ctcgccgcgc tgcaggcgcc
     2161 gtggagcgtg atcgatggcg cgctggtcga ccgcatcgac gtgctcggcg gcggcttcac
     2221 cgccgatcgc ggcgatcgcc gcggcggctt cgtcgagatg tccacgtcgc tgccggacgg
     2281 tccggagcgg atgcacgccg ccgtggggtc gctgcagagc ggcttctcct acggcgcgcc
     2341 gaccggaagc ggctcgctgc tcgtcgccgg ccgggcctgg tatccggaag cgctggggtc
     2401 gacgatcgag atcggcgagc ccggcctcga tccgcgcttc gccgatctct acgtcaagtc
     2461 ctcgttcgtg gtctcgccgc agaccgccct gtcgctgcac gcgctcgccg gttcggatcg
     2521 actggagttc cgcgagagcg ccggcaacga gcaggtcgcc ctcgtcgaga agagcaccca
     2581 cctctgggtg cggctgctcc aggccccgac gccgaagctg ctgttggaga cggtggcgtc
     2641 gggggggcgc ctgacgcgct cccgcgtcgg cgcggcggag ccggaggacg aggtgatctc
     2701 cgtggacgac gatcggacca tcgatttctt cggggtccgg agcgacgtga cgtgggccgt
     2761 ccgggaagat cgcctcttga agttcggcct ccaggcgcgc cccatgtctt ccgattaccg
     2821 ctacgcgacc ggaccggctg gcgatccggc ggccgggaca tcgctgcgcc tcgatccgca
     2881 ggggacgtcg ttcgccgcct acgccgcctg gcggaccggg ctctccgacc gcgtcgtcct
     2941 cgaggccggg ctccgctggg accggcagac ctacacggac gacgacgaga tgagcccgag
     3001 gctgaacgcc gtgtggcgcg cctccgaccg cacgacgctc cggctcggcc tgggtcgttt
     3061 cacccagtcg caacgcctgc acgagctgga catcgaggac ggcgagtcga tgttccatcc
     3121 ggccgagcgc agccggcagg ccgacctgac gctcgagcac cgcacggatg cggggctgcg
     3181 tttccgcttc gacggttact actgcgccat cacacgcccc cagccgcgct ccgagaacct
     3241 gttcaatccg atcgagctct tcccggaggt gtctcccgac cgggtgcgga tcgccccgga
     3301 cggcgcgcgc ctccggggag ccgagctgtt cctcggcggc gacccacagc accgcttcgt
     3361 ctggtgggga agctacgcct ggtcgtcggc ggaggaccgg gtggacgggg ccaccgcgcc
     3421 ccggagctgg gatcagacgc acgcggtccg gctggccgcg gcctggcgcc cggacgcgcg
     3481 gtggaccatc gcgctgctcg gcaccgcgcg ctccggctgg cccaccaccc cggtcacggc
     3541 ggagagcgag ctccagccgg acggctcgac ggagatcgtc gcgacaccgg ggaggcgcaa
     3601 ctccgaccgg ttcccgtact acgcgcggct cgacctgaaa gggagcgtcg tcctcccggc
     3661 ggcgcgcggc cgcctgcgcc tcgagatcga cgtcaccaac atcaccgatc gggcgaacgt
     3721 ctgctgcgtc gacgagttcg tcttcgacac cctccccgac ggcagcatcg acacccggac
     3781 cgatctcgac cactggctcg gcgcgacgac cgccgtcaac gtcgagtggt cgttctgacg
     3841 ggaggggacg aacccgatcc gccgggcgtc gcctgggctc ctagagggtt cggctcttct
     3901 ccagcgtctc cgtgagggtg gagaccacct tcgcctcttc ctgcttcgtg agaccgagga
     3961 tctcgcggag ggcgatgccg atgtggggga tgtacgactt gatgtaggac tgcttcttca
     4021 tctcctcggc gagccggcgc cggtggcgca gccagccgcc gagtcggcgg ccgacctcca
     4081 tcagccccag gcgcagctcg cgcaggatct ccgggtagcc ggccaccgcc tccttgctct
     4141 cggaggtgaa cggcacccag accgacgcca tgtggacgaa caggatcatc ggcccggtcg
     4201 gcagcgcccc gcgcgactgc tgcatggcgt agttgcgcca gtcggtggtg acggcggcgc
     4261 gggtgatggc gcaggccgac tgcaggtact gcagcggcac ccggttggcg aaacgccaca
     4321 gatcgaccgg ctcctccccc ggcaggtcgc cgccgtaggc cagcccgacc tcgatcagga
     4381 acgggttgcc gcggtacacc gccggcggcc gcgtcgccga cgcgtagaag tcggccttca
     4441 ccgtctgctt cagcccctcg aggatgagct cctcgccgat cggcacgatg acgttggtcg
     4501 gtggcgccat gatcttgacc ttggggatgg cgcggaagag cgactcgatg tcggcggtcg
     4561 gcagcgagcg cggcctcgcg ttcgggttga ccttcgcctc cttgcagatc tccagcgcca
     4621 cccggtcgct gacccgggcg aagtcggact ggagcgcggt ggagaccgtg cggctcttgc
     4681 tgtcctgcag catggcgatg aggatgccga gctccacccc gtacgggtgc ggcttgatca
     4741 ccagcggctc gcgcggcagc tccttggtca gacgggcgcg gtggaacggg tcccctttcg
     4801 gtggcgtgta gtgcacgtcg gcgtgcgggt tggccagagc cgtctggatg acgtagtcgt
     4861 ccaccgaccg gcgccccttc ttgtagaggc cggccagctc catctcgacc ttcgtgccgt
     4921 gatcgcggtc ccactccacc tcgcgatcga cgatgatctt gggctcgttg cgcgtcgtgt
     4981 cgatggagat ctcgaagtag tgcgccggtt tcttcttccc ggtgcgcgag gtgatgcgca
     5041 ccggctggcc ggtggtgagc tgtgagtaca tcccggccgc cgagatgccg atcccctgct
     5101 ggccgcggct ctgccgcatg gtgtggaact tcgagccgta caggagcttg ccgaagacct
     5161 tcgggatctg gttgcggacg atgccggggc cgttgtcggt gatggcgagg cggaagcggt
     5221 cctccgcctt ctcctcgatc tccacctcga gcgccggcag gatccccgcc tcttcacagg
     5281 cgtcgaggga gttgtccacg gcttccttgg tcgctgtcag gagtgccttc tggggattgt
     5341 cgaagccgag gaggtggcgg ttcttctgga aaaactcggc gaccgagatc tcccgctgcc
     5401 gcttgccgat cgactcggcg gtctcccgcc gccgcgccgg aggagtctcc cctccgccgg
     5461 atgccgccgc ggccggctcc ggcgccgggg gggcttcggg ctcagcttcc gttctggggg
     5521 ccgccggcgt cggacgggcg cccctccggg atccggcgtt ccccttcggt tttcctgccg
     5581 gcttcgcccc gcgcccgggc ttcgcggcga ggatctcgcc ccgcttcgcc gccacctcgg
     5641 agccgcgacc ctccggccgc tccgggcgcc gcgccgcggc ggcggggtgc ggcgccgcga
     5701 ggaacagatc catctgcccg gcccccttcg gcgccccttc cgtcttcatc ctcgtcgtca
     5761 ctcgccctcc gctcgccgcg gcgccgttcc gaacaccacg cgcggcgcga ttctaccgtg
     5821 ccgcgtggat aataagcgga cttcgagccc aacgatacgc ctgctgcgag agccgcttct
     5881 gcatttcttc gtcctcggcg cggcga
//
LOCUS       DASRIQ010000041         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2097728, whole genome shotgun sequence.
ACCESSION   DASRIQ010000041 DASRIQ010000000
VERSION     DASRIQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097728"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..872)
                     /locus_tag="VFD06_01035"
                     /pseudo
     CDS             complement(1..872)
                     /locus_tag="VFD06_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191509.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
     gene            complement(892..1176)
                     /gene="gatC"
                     /locus_tag="VFD06_01040"
     CDS             complement(892..1176)
                     /gene="gatC"
                     /locus_tag="VFD06_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZN02148.1"
                     /translation="MAISLDEVRRIARLAHLAFGPEEQERFTRQLSAILDHVKMLERL
                     DTKEVEPTAHAGTGAALRDDQIVPPLDEETALENAPEAERGLFKVPRVLG"
     gene            1234..2175
                     /locus_tag="VFD06_01045"
     CDS             1234..2175
                     /locus_tag="VFD06_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02149.1"
                     /translation="MIALSFPHLLRAALLLAATGADPALVPLAPERTDGAAVRVLIET
                     MVIDRRGTWSAGADQADLIPGSAGILKKSITLEAKDGRRTKEPIEVVARLIPGVLAPG
                     GPACALALDIETRAASAAPGKTVGRVTSTLSLAAGEERFVDAYVSPKSGNKVAFRVRC
                     VEPRHEEDGSPEMVAVDLEIERRVEGESPSVIGDQKLVALLGREVAATVSDHTTLSGD
                     GEGDGDEGARFRRERLDVTLSPIVTVAGKLQIEVRVSGEVATVGASGKALAHPIDRTE
                     VFLVSSLEARSFEIVLPGSAAEGWTDLRFLVQIRPRF"
     gene            2172..2876
                     /locus_tag="VFD06_01050"
     CDS             2172..2876
                     /locus_tag="VFD06_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HZN02150.1"
                     /translation="MKGRRARGAGRRPERAPLHAWYRALLRAYGPQGWWPGRTRFEVI
                     AGAFLTQNTAWSNVEKGLRSLRRAGLLHPGRMHRAGPGVTAPLLRPTGYFNQKAKRLH
                     AFLDFLVAEHRGRLDRLFALPLPNLRERLLRLEGVGPETADSIILYAGRRPVFVIDAY
                     TRRILKRHGHLHPHETYEDLRRRFEAALPSEVPLYNEYHALIVRLAKDRCRSRIPDCR
                     GCPLERFLPPSGPRRD"
     gene            2975..>3231
                     /locus_tag="VFD06_01055"
     CDS             2975..>3231
                     /locus_tag="VFD06_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02151.1"
                     /translation="MTSRWQSRTRIDLPLRILFFFSILLVAGDRRTLRAQPAAAAEPV
                     MSAAAPLIGRLTDLDGMAVAGATILIFRTGEPGWPIDTAGA"
BASE COUNT          420 a         1166 c         1146 g          499 t
ORIGIN      
        1 ccctgccgga cggcgcgctc gacttccggg tccatcccgt cggggaagta ttcgtccggc
       61 agcccgagcg tgagaccggc cacccccttc gtcagctcgg cggcgtagtc gggcacgggc
      121 gcctcgatcg aggtggcgtc atgcgggtcg tggccggcca ccgcgccgag caggagcgcc
      181 gcgtccgtga cgtcctgcgc gaacgggccg atctgatcga gcgaggacgc gagcgccacc
      241 agtccgtagc gggacacgcg gccgtaggtc ggcttcatcc cgacgacgcc tcacagcgcc
      301 gccggctggc ggatcgagcc gccggtgtcg gtgccgatcg cgatcggcgc ctcgtcggcc
      361 gcgaccgccg ccgccgagcc tccgctcgag ccgcccggga cgcgggccgt gtcccacggg
      421 ttgcgcgtcg tccggtaggc ggaattctcg gtcgaggagc ccatggcgaa ctcgtccatg
      481 ttggtcttgc cgaggaccat cgcccccgcc cggcgcaggc gcgcgaccag ggtggcgtcg
      541 aatggcggga cgaacggctc gagcaccctc gagccgcacc gggtcggcag gccggcggtc
      601 gccaggatgt ccttgagcgc gaccggcacg ccggcgaggg ggccgagcgg ccggccggcg
      661 cggcgatcgg cgtcgatccc ctcggcggcg gcgcgcgcgc cgtccgcgtc cacggtcacg
      721 aaggcgccga ccgcctcgtc gacggcggcg atgcgctgca ggcacgcgtc gaccgcggcg
      781 gaggcggtga tctcccccgc gacgatccgc tcccggagcg ccgccgcgcc ccagcgggtg
      841 ctgtctgcgc ctgcgtcgcc cgcgacgctc atacgaagcc caggagcgcg ctcatccgag
      901 cacccgcggc accttgaaga gcccgcgctc cgcttccggc gcgttctcga gcgccgtctc
      961 ctcgtcgagc ggcgggacga tttgatcgtc gcgcagcgcg gcgccggtgc cggcgtgcgc
     1021 cgtcggctcg acctccttcg tgtcgaggcg ctcgagcatc ttcacgtgat cgaggatggc
     1081 gctcagctgg cgcgtgaagc gctcctgctc ctcggggccg aacgcgaggt gcgcgaggcg
     1141 cgcgatgcga cgcacttcgt cgagggagat cgccatgccg gaagtatacc cggcgatcgc
     1201 tgtggtagtc tcggcgcgga ccgcaacgaa ttcgtgatcg ctctgtcgtt cccgcatctt
     1261 ctgcgcgccg cgctgcttct cgccgcgacc ggcgccgatc cggcgctcgt cccgctcgcc
     1321 cccgagcgga ccgacggcgc cgccgtccgc gtcctgatcg agacgatggt gatcgatcgg
     1381 cgcggcacct ggagcgccgg agcggatcag gccgatctca tcccgggatc ggccggcatc
     1441 ttgaagaagt ccatcacgct cgaggcgaag gacggccgcc gcacgaagga gccgatcgag
     1501 gtcgtggccc gtctgatccc cggcgtgctc gcgccgggcg gcccggcctg cgcgctggcg
     1561 ctcgacatcg agacacgcgc cgcttccgcg gcacccggga agacggtcgg gcgcgtaacc
     1621 tcgaccctct ccctcgcggc cggcgaggag cgcttcgtcg acgcctacgt gtctcccaag
     1681 agcggcaaca aggtggcgtt ccgggtgcgc tgcgtcgagc cgcgtcacga ggaggacggc
     1741 agccccgaga tggtcgccgt cgatctcgag atcgagcggc gcgtcgaagg agaatccccg
     1801 tcggtcatcg gcgatcagaa gctggtggcg ctcctgggcc gcgaggtggc ggccaccgtg
     1861 agcgatcaca cgacgctgtc cggagacggc gagggggacg gagacgaggg ggcccgcttc
     1921 cgccgcgagc ggctcgatgt cacgctgtcg ccgatcgtca ccgtcgccgg caagctgcag
     1981 atcgaggtgc gggtctcggg agaagtggcg acggtcggcg cgtccggcaa ggctttggcg
     2041 catccgatcg accggaccga ggtcttcctc gtatcgtcgc tcgaggcgcg gtcgttcgag
     2101 atcgtccttc ccgggagcgc ggcggaaggg tggaccgacc tgcgcttcct cgtccagatc
     2161 cgtccccgct tttgaaaggc cgccgcgcgc ggggtgccgg ccgacgcccg gagcgggcgc
     2221 cgcttcacgc ctggtatcgg gccctcctgc gcgcctatgg tccgcagggg tggtggccag
     2281 ggcgcacgcg cttcgaggtg atcgccggcg ccttcctgac gcagaacacc gcctggtcga
     2341 acgtcgagaa ggggctccgg agcctccggc gcgctgggct cctccatccg ggccggatgc
     2401 accgggccgg ccccggtgtc accgcaccgc tcctccgccc gaccggctat ttcaaccaga
     2461 aggcgaagcg gctgcacgct tttctggact tcctggttgc cgagcacagg gggcgcctcg
     2521 accggctgtt cgcgctgccg ctcccgaacc tgagggaacg gctcctgcgc ctggaggggg
     2581 tcggtcccga gacggcggac tccatcattt tgtatgccgg gcgccggccg gtcttcgtca
     2641 tcgacgccta tacgcgccgc atcctgaagc ggcacgggca tctccatccg catgagacct
     2701 atgaggatct gcgccgccgc ttcgaggcgg cgctcccctc ggaggtcccg ctttacaacg
     2761 agtaccacgc gctcatcgtg aggctcgcca aggatcgctg ccgatcccgg attccggatt
     2821 gccggggctg tccgctggag cgcttccttc ccccgtccgg accccgtcgt gattgactca
     2881 aattcttgct tcgattagag taagccgctt tcctggcgcc ggttgcgcgc actcttcggt
     2941 cgttcgattc gggaaattgg acggagggcc gcggatgacg agtcggtggc agagccggac
     3001 ccggatcgat ctcccccttc gcatcctctt cttcttctcc attctcttgg tcgcgggtga
     3061 ccgccgcacg cttcgcgccc agccggccgc cgcggcggag ccggtgatgt ccgccgccgc
     3121 ccccctcatc ggacggctga ccgatctcga cgggatggcg gtggccggcg cgacgatcct
     3181 gatcttccgg accggcgagc ccggttggcc gatcgacacc gccggcgccg a
//
LOCUS       DASRIQ010000042         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2627631, whole genome shotgun sequence.
ACCESSION   DASRIQ010000042 DASRIQ010000000
VERSION     DASRIQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627631"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFD06_01060"
     CDS             <1..412
                     /locus_tag="VFD06_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02152.1"
                     /translation="RLSGIYARAGASFPIFSGTECFPYRLTGGGAAAFWYFTDGPSYG
                     AKLRTYAHGTLACVVHARADLTLLGGLTDDVWHLAGHGFAAGGIGSCEPEDWDSRREV
                     LRDSWCFACVIDGEFRTDSESDEFEGDLRGPDCD"
     gene            423..>3102
                     /locus_tag="VFD06_01065"
     CDS             423..>3102
                     /locus_tag="VFD06_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02153.1"
                     /translation="MAFGAALAVLLLAPAGSLHSEPAPGARYLLSATQGGGATAPKVL
                     LRWSAADRPRPYPDGGLYARYLVRRRAETAASLADLAVVEPLTDAADIDALFAGGGTP
                     DPSTLREELTALLVPGVAPTLGEALLALRLSADPGRRMQYQMILAGNHRAAMAAGLAY
                     LDDGLTDGTRYLYEVWGLDDLNVAVERLGKIWVKAGVDTVLPAPVALTVVEVADADNP
                     PLGDFPGIGDLDLDGDGRIDGGAFGDGKIYLKWDPTPGQAKDRETQTSGFGFDVYRDL
                     KPAGPCPATPPLPPGGAAVKVNTVPVLPLPATGDDPATPAPDPELPDFFFVDDNGNDL
                     VPPTVRGTSYCYWLVARDLLRQPGAWSAPVVGCAPDRGRPRQVRDVRTEIVSPAQVQV
                     SWTANASDPPTAVGGSPYVDDTVAYNVYRFTDRSRLLGPPLPVDRVATGLPAGTISWT
                     DVTPASPSSNRGKLYWYTVTATDTGLCGRPANESAHSAPARAVVYDETPPAIAAIAPF
                     CAPLTSSLNPRCYRDCGGTGLPPAIAAWCHAGGASGLFPRAGLDAWGFSVLPASLPSD
                     NWTVRLYRAKGCSTGTDFRPVEEDDLAPGESEYLLPDNAFQPRVSQKIEYRLRALDRD
                     SNLGASTTPRTFSNQPLPVYLRSAPPARPTIVQTIPAGGGNVKVRWHAPGSEGLAGFV
                     VRVGPAADRLEGDFSHLPAGNLCAETSDTAPCIAEDADFDGVPSPYSIVIHDDRLGPG
                     TATKCLAEFPNLPGFKVGEYFEHTVAAPARGEAIEVYAADITGQLSEPAIAPNATLVP
                     DFTPPWPERDLPPTFDVLSAVVPAGGGMPLHVDLCWDQGAHGRCFDTQEICDEAADCP
                     GSVCVTSLDPYPHVAVFRTRVEAGRPDSFQQRSPLLN"
BASE COUNT          404 a         1177 c         1080 g          441 t
ORIGIN      
        1 tcgcctcagc ggcatctacg cccgcgccgg cgcgtcgttc cccatcttca gcggcaccga
       61 gtgcttcccg tatcgactca ccggcggcgg agcggccgcg ttctggtact tcactgacgg
      121 gccgtcgtac ggggcgaagc tgcgcaccta cgcgcacggc acgctcgcct gcgtcgtgca
      181 cgcgcgcgcc gacctgactt tgctcggcgg actgaccgac gatgtctggc atctggcggg
      241 gcatggcttc gcggccggtg ggatcggctc ctgcgagccg gaggactggg acagccggcg
      301 cgaagtgctg cgcgactcct ggtgcttcgc ctgcgtcatc gacggcgagt tccgcaccga
      361 ctcggaatcg gacgaattcg agggggacct gcgtggcccg gactgcgact gatctccgcc
      421 gcgtggcgtt cggcgccgcg ctggccgtgt tgcttttggc cccggccggc tcgctgcact
      481 cggagccggc gccgggggcg cgctacctgc tgagcgcgac gcagggcggc ggggcgaccg
      541 cgccgaaggt cctgttgcgc tggtcggcgg ccgatcgccc gcggccgtac ccggacgggg
      601 ggctctacgc gcgctacctc gtccgccgcc gcgccgagac cgccgcctcc ctcgccgacc
      661 tcgcggtcgt tgagccgctg accgacgccg ccgacatcga cgccctcttc gcgggcgggg
      721 gaacccccga cccgagcacg ctgcgcgagg agctgaccgc cctcctcgtg cccggcgtgg
      781 cgccgaccct cggagaggcg cttctggccc tgcggctgag cgccgacccc gggcggagga
      841 tgcagtacca gatgatcctc gccgggaacc accgcgccgc gatggcggcc gggctcgcgt
      901 acctcgacga cgggctcacg gacgggacgc gttacctgta cgaagtctgg gggctcgacg
      961 atctgaacgt cgcggtcgag cggctgggga agatctgggt caaggcgggc gtggacaccg
     1021 tcctcccggc gcccgtcgcg ctgaccgtgg tcgaggtggc ggacgccgac aacccaccgc
     1081 tcggcgactt cccggggatc ggggacctcg atctcgacgg tgacggccgg atcgacggcg
     1141 gggcgttcgg cgacggcaag atctatctca agtgggatcc caccccgggg caggcgaagg
     1201 atcgggaaac ccagacctcc ggcttcggct tcgacgtcta ccgcgatctc aagccggccg
     1261 gcccctgccc ggcgacgccg ccgctcccgc ccggaggggc cgccgtcaag gtgaacaccg
     1321 ttccggtcct gccgctgccg gcgaccggcg acgatccggc gacgccggcc cccgatcccg
     1381 agcttccgga cttcttcttc gtggacgaca acggcaacga cctggtgccg ccgaccgtgc
     1441 gggggacttc ctactgctac tggctcgtgg cgcgcgatct cctgcgccag cccggggcgt
     1501 ggagcgcccc ggtcgtcggc tgcgcccccg accggggccg cccgcggcag gtgcgcgacg
     1561 tgcggaccga gatcgtgtcc cccgcccagg tccaggtgtc ctggacggcg aacgccagcg
     1621 atccgccgac ggccgtcggc ggcagcccgt acgtggacga caccgtggcc tacaacgtct
     1681 accgcttcac cgaccggtcc cggctcctcg gcccgccgct gccggtggat cgggtcgcca
     1741 ccgggcttcc ggccggcacc atctcctgga ccgacgtcac gccggcgagc ccctcgagca
     1801 atcgcgggaa gctgtactgg tacaccgtga ccgcgaccga caccgggctg tgcggccggc
     1861 cggcgaacga atcggcgcac tcggccccgg cgcgcgccgt cgtctacgac gagactcctc
     1921 cggcgatcgc cgccatcgcg ccgttctgcg ctccgctcac cagctccctg aatccgcgct
     1981 gctatcgcga ctgcggcggc accggcctgc cgccggcgat cgccgcctgg tgccacgccg
     2041 gcggcgcttc cgggctcttt ccgcgcgccg ggctcgacgc ctggggtttc tccgtcctgc
     2101 cggcgagcct gccgtccgac aactggacgg tccggctgta ccgcgcgaaa gggtgctcga
     2161 ccgggacgga cttccggccg gtggaggagg acgacctggc gccaggggag agcgagtacc
     2221 tcctccccga caacgctttc cagccgcgcg tgtcgcagaa gatcgagtac cggctgcggg
     2281 ccctcgatcg ggattcgaac ctcggagcca gcaccacgcc gcgcaccttc tcgaaccagc
     2341 cgctgccggt gtacctgcgc agcgcgccgc cggcgcggcc gacgatcgtg cagaccatcc
     2401 cggccggcgg agggaacgtc aaggtccgct ggcacgcgcc gggttcggag gggcttgccg
     2461 ggttcgtcgt ccgggtcggt ccggcggccg atcgcctcga gggggacttc agccacctgc
     2521 cggccggtaa tctgtgcgcc gagacgagcg acaccgcccc ctgcatcgcc gaagacgccg
     2581 atttcgacgg cgtcccgtcg ccgtacagca tcgtcatcca cgacgaccgg ctggggccgg
     2641 ggacggccac gaaatgcctc gccgaattcc cgaacctccc cggcttcaag gtcggagagt
     2701 acttcgagca caccgtcgcc gccccggcgc gcggcgaggc gatcgaggtg tacgccgccg
     2761 acatcaccgg ccagctgagc gagccggcaa tcgccccgaa cgccacgctg gtccccgact
     2821 tcaccccccc ctggcccgag cgcgacctgc cgccgacgtt cgacgtgctt tcggcggtgg
     2881 tcccggccgg cggcgggatg ccgctgcacg tcgatctctg ctgggaccag ggggcgcacg
     2941 ggcgctgttt cgacacgcag gagatctgcg acgaggctgc cgactgcccc ggctccgtct
     3001 gcgtcacgag cctcgatccg tatccgcacg tcgccgtctt caggacgcgg gtcgaggcgg
     3061 ggcgccccga ttccttccag cagcgcagcc cgctcctcaa ca
//
LOCUS       DASRIQ010000043        13054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2098558, whole genome shotgun sequence.
ACCESSION   DASRIQ010000043 DASRIQ010000000
VERSION     DASRIQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098558"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFD06_01070"
     CDS             complement(<1..387)
                     /locus_tag="VFD06_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02154.1"
                     /translation="MKHFVILAACLVPAAAFAQEAKTYTNADLVKIQVPGAYTNEDLK
                     RLPPLPVQKGPKVAAAPQVEAAPAPATEAFQAYYNSVRADRDILAAELAYEIGLVEFS
                     ESAFAGDTRSFDVRLGYRSQAVELIRE"
     gene            813..3482
                     /locus_tag="VFD06_01075"
     CDS             813..3482
                     /locus_tag="VFD06_01075"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZN02155.1"
                     /translation="MALYEDHSLPLVAVSVWVGSGSKDEAETSAGYAHFLEHMIQRGT
                     ESSGPFEYTRRAQRWGGSLSVRANYDRTAITLSGVPETLAEMIDAAASMALRATLKDK
                     EIDQELGTLMQEMRTYYDLPSSVALFETMRAAFPKHPYRVPTLGNFRSVGTLKSEPLT
                     AFYRNLYVPNNMTLAVAGDFEPRAAAARIEAAFGAASKSATLPPRPVPPGAFAGHTDV
                     EKKLDFKESWTALAFMGPGYRHPDRVAFEALATALADPSSPLLLASARDQAGALAQAS
                     FYGLEDAGLLYVALNPATPQLSYSAATSALRAIVAFRAAGFSDAQARDLRERMLRDER
                     QRAASMTERAERLGEATLFGGVRYYWDRPVVVSRLTAVELNRVAAKYLVPDNCRLVVL
                     VPKETADFPEETKAEFHKVFDTLKGPEQESPAGLDATLYPPSEGAKVDAAAWGKGGGA
                     APLGAPRRTVLKNGLTLLVLEDHRMPLAALSLQLRGGSGSDEPGKEGRASLLLRLLGT
                     RTAQALREAATGGGARRPLLPPEAQAGRDLLELRLSADPSDVPLGLEAIAKAVREGPA
                     SADLERVRQAALQTLGRAEGRADVIGFDLFHEKVYSGHAYAQRPDGTPAGLQAVGLAD
                     LQTLAATLLDPASSVLAVAGDVDAADVGRAVERLFGPWKGSGAKRRDSDVAAAPRAAP
                     AAPAPAAEGEETTPQAPVFAATAGAQPGEYARQVGSSQSLAIVGVPGVPLLDGDFEAL
                     RYLGAAVTLFGFEEMVFERRAAFSVSSSPEGFKEGGALAFEAAAAHARRGEAQFELQR
                     LMRRLAVEDLGDADRRDLAHIQAGRAAAAAQGTQALASLLAYREAAGLGAANWRAPVT
                     VSTPPTAARLRALAERLFQPERTIRVIVGPPSP"
     gene            3493..4461
                     /locus_tag="VFD06_01080"
     CDS             3493..4461
                     /locus_tag="VFD06_01080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN02156.1"
                     /translation="MSESPVLSIVLPVHNEADTLRDSLGRIREVLLRLGRPFEILAVD
                     DGSTDRSGAILAEMASSEPRLIGLTLTRRFGKEAALAAGLSAARGEAVILMDADGQHP
                     PELIPEMVRLWEEGIDVVEGVRRDRGPEGIPHRLLARAFYGLLGRAVGRDLQGATDYK
                     LLDRQAVEALLECGERNRFFRGLVAWIGFRTAEVPFLAPERHGGTSKWSLADLLRYSV
                     RSLLAFTSLPLRIVAGAGLVMVLLGAILALQTLWNWSSGHAVSGFTTVILVQLILGGL
                     VLTSLGVIAAYLALLYEEQKGRPIYLVRRPRGRPARPGPSPGALGS"
     gene            complement(4458..4976)
                     /gene="moaC"
                     /locus_tag="VFD06_01085"
     CDS             complement(4458..4976)
                     /gene="moaC"
                     /locus_tag="VFD06_01085"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZN02157.1"
                     /translation="MRGAGPRLTHLDRRGAARMVDVGDKPVTARAARASGEIRMSAAA
                     FRALRSRTAKKGDALALARTAGIAAAKRTSEWIPLCHVVPLDQVTVEIAFAPRRRALV
                     VEATARARWRTGVEMEAMVAVAAALLTLYDMAKGMDRGMRLGPIRLEEKSGGRSGPFR
                     ARRRSRAPDARS"
     gene            complement(4976..5590)
                     /locus_tag="VFD06_01090"
     CDS             complement(4976..5590)
                     /locus_tag="VFD06_01090"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZN02158.1"
                     /translation="MSQIASTAFTGLVLAGGRSTRMGRDKASLPIAGRTLLLRAVETI
                     RRAGGTPVVLGPPRPESESHGARFLDDPGEGPLQALRVGMSGTPGPWFALACDLPLLK
                     AEAIGALVAGLEDHDAVVPRALGRLQVLAAAYAPPALAAFERAIATGECAMQAAVAGM
                     RVRVVDPDALREWGDETMFLNVNTPDDLRDAADRLAAGHPERAA"
     gene            5649..7028
                     /gene="mgtE"
                     /locus_tag="VFD06_01095"
     CDS             5649..7028
                     /gene="mgtE"
                     /locus_tag="VFD06_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZN02159.1"
                     /translation="MPAQRSVAKGQGDRPYGRVVRRLLHVGARDDVGRLVDRMLPADI
                     VDLLRSLGPGERRLFLGILSQHDAAASVFALLPPPLRTEVLEQATDEAIASLVARQPP
                     DEAADLLGLLEASRADAVLHLLDEKTAANLDRLMTYGRDTAGGMMTTRFIALPGATTV
                     AEAIARLRAGAQDETVFYVYAVDEAGRLQGVVSLRQLVLGRPEQTIEEIMNPRVLSVR
                     TDEPRGRVADLIARYNLLALPVVDDRNVLCGIVTVDDAIDAMADETTREMYRMAGLDT
                     EDRVTSPASVSVRRRLPWMVINLGTAILAATVVSFFEDTIAQVVALATLMPIVAGMGG
                     NGATQTLTVIIRGIALGDLDFSTARQAILREITVGAAIGAVTGILMAGVAVFWKGNPV
                     LGLVIGVAMVINLFVAGFAGATVPILLKWLRLDPALGSGVVVTTFTDCCGFLSFLGLA
                     TLLLRHLTP"
     gene            complement(6958..8037)
                     /locus_tag="VFD06_01100"
     CDS             complement(6958..8037)
                     /locus_tag="VFD06_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN02160.1"
                     /translation="MDEGPTPLPAPRLPQPTDSRVGPDLGLEGHRRSDDWHAAHLYAP
                     EIVVPGSRMPASRHLFLPGASLPPRLNDDGRALVAWLQGLGRARRDVWSELRAQDPPV
                     PEPSPGSVEARQARGAALYAAWCVPCHGAAGDGKGPAAALLAIPPRDLRRAGFRFRSV
                     PLESPATDADLFRVLTLGSGTGAAMPSFAFLDPEDRWALVQAVRDFSTATRGAALRSD
                     RAASAIPDVTVVPAPAADAARGARLYAAWGCAACHGADGEGKALGDAGEGGDGTAEPV
                     VRASDLRHACARRGGGSPAAFERALRFGTGPTMPSFAAVLDREPGAVAAILAWLESRD
                     PEVRASGGGAAGSPDRGTTGSRSSR"
     gene            complement(8241..9176)
                     /gene="cyoE"
                     /locus_tag="VFD06_01105"
     CDS             complement(8241..9176)
                     /gene="cyoE"
                     /locus_tag="VFD06_01105"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZN02161.1"
                     /translation="MSRSESHADPVARTARQEVAAAARARLADYLMLTKPRVTAMVLL
                     TTLAGFYMGSTRGLDAGLLLRTLFGTALAAAGASALNQYLERDGDGRMRRTRGRPLPG
                     GRLDPQRALLFGSILATAGILFLAPAVNALAAGLVALTLLTYVLLYTPLKTRSPLCTL
                     VGSLPGALPPLVGSVAAEGRVTAVGVGLFAILFVWQLPHALAIACLYRDDYDRGGYLM
                     LPVVAGDNAATGRIALLYALALVPVTLLPVVWGGAGPLYLGGALLLGGLHAVAAAPLA
                     RTGSLPAARRLLLASVVYLPVLLSLMAFDRQPGPR"
     gene            complement(9173..10120)
                     /locus_tag="VFD06_01110"
     CDS             complement(9173..10120)
                     /locus_tag="VFD06_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZN02162.1"
                     /translation="MRASIPYDPWRHRYALGLLAATVVLLLAGGLVTSTGSGLAVPDW
                     PLSFGQVFPEMRGGVLFEHGHRLVAATVGLMTVVLAFWFGRREPRPGVRRLAFLALAA
                     VVAQGLLGGLTVLLRLPAAVSVAHACLAQIVFAIVVALAVTTSRPFFDEARAASPVPA
                     GVGLLAGIATAATYAQVVLGALMRHTGAGLAIPDVPLAFGRIVPPILSFEIGVHFAHR
                     VLALVVAGLVAALAIRCAPLRRHPAIALPARIATTLVLLQILLGGAAVLTRLAIVPAT
                     LHVVTGALLFGTLLATTLFAWRRRAPSAAADLRPSEVRA"
     gene            10213..11193
                     /locus_tag="VFD06_01115"
     CDS             10213..11193
                     /locus_tag="VFD06_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN02163.1"
                     /translation="MREIPSVTAIRAEGLAFDYGRERGLDGVDLEVAAGERFAILGPN
                     GGGKTTLFRILATLLKPARGRAAIFGHDVATSADAVRRALGVVFQAPAVDRLLTVREN
                     LDLQGALYGVRGAALQREREALLDRFGLADRFRARAGTLSGGLLRRLEIAKALLHRPR
                     LLLLDEPSTGLDPQARADLWQALDDQTRRDGTTVAFTTHLLDEGDRSDRVAILDRGRV
                     VALGTPDALKAEIGRQVLVVAVEAPGDPGLRAEVERLTGHPAREAEGRILLDAPDGAR
                     LVPLLSEALGGRIGGISLSRPTLFDVFLRRTGHRFDAAPPQDFRGAGDAA"
     gene            11190..11984
                     /locus_tag="VFD06_01120"
     CDS             11190..11984
                     /locus_tag="VFD06_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02164.1"
                     /translation="MSRAAAATLALAGRETRRFFRERSRVTGALLQPVLFWAFFGAGL
                     APSFHLGGGIAYGAYLLPGIVVLVLLFTAIFSTISVIEDRREGFLQGVMVAPVPRGAI
                     VAGKILGGTILATAQAGLALLLAPLAGVPLSAAAFAAAVAFCFVLGFGLTSLSLAIAW
                     PMRSTQGFHAIMTVFLMPLWLLSGAFFPAEGVPRWLGLLMRADPLAYGVAALRRILMP
                     LPGVPAAADAGATAAAVEIGLTFLFVVAAFCAAVAVTRRRDGALPG"
     gene            11992..12408
                     /locus_tag="VFD06_01125"
     CDS             11992..12408
                     /locus_tag="VFD06_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HZN02165.1"
                     /translation="MPLPAFNALMNALSALCVVAGWWLIRNGRPRAHRTAMLLALLAS
                     TLFLGGYLTHHARVGSVPFRGDGAWRTLYFSILIPHTLGAVAVLPLVLVTVARAARGR
                     FEAHRRLARVTLPLWLFVSVTGVAVYVLLYHGPGVR"
     gene            12405..>13054
                     /locus_tag="VFD06_01130"
     CDS             12405..>13054
                     /locus_tag="VFD06_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZN02166.1"
                     /translation="MRRTGLQAALVMFARSAAPGRVKTRLEPRLGRQGALRLHEALVA
                     DSTDLLRLAARRARARPFVAWTGRPPRRAAVLRQGSGDLGARLRRVVRRLLCAGHRAV
                     VVIGSDSPALPAARIVRAVRLLERGADLVLGPAIDGGYYLIGLRSDQPALFDRMPWGG
                     RRVAARTLERAARLGLRTERLPPYFDVDRPADLVRLRRALSGPRRRRAPRTARLLL"
BASE COUNT         1669 a         4864 c         4755 g         1766 t
ORIGIN      
        1 ctcacggatg agctcgaccg cctgtgagcg gtagccgaga cgcacgtcga aggagcgcgt
       61 gtcccccgcg aacgccgatt cggagaactc gaccagcccg atctcgtacg ccagctccgc
      121 cgcgagaatg tcgcgatcgg cgcggacgct gttgtagtac gcctggaagg cctcggtcgc
      181 cggggccgga gccgcttcga cctgaggcgc cgcggcgacc ttcggtccct tctgtaccgg
      241 cagcggcgga aggcgcttga gatcctcgtt ggtgtacgct cccggcacct ggatcttcac
      301 caggtcggcg ttggtgtacg tcttggcttc ctgcgcgaat gcggcggccg gcacgagaca
      361 ggccgccaga atgacaaaat gcttcatgaa tcctcccatg cgcccggggt ccgccggtcg
      421 cgggggcgtg gccgccctcg cgtgactgca tccggtgtgg gccgtcccga ttcggcccgc
      481 ggacaccctc gcgcggtgtg cggaccatat acgccgaccc tccccctccg acaacgattt
      541 tttcctcgcc gcatggctcg aaatccttgc ctagagcgga ttctggccga ttcacccggg
      601 tgtcaagaaa ccgccgcact tccgcgcctc cggcggcgcg caattgaaac cgaccggcgg
      661 cggccgctat aatccgcccc tttcacgggg ggtgtcccat ccgcgcgcga ctcgtcctgg
      721 ccctgctcct gtcctgcggc ttcgccgccg ccgcggcacc cgcgttcgcg gccggcgcgc
      781 cggagacgcg taccctcccg aacggcctgc gcatcgcgct ctacgaggac cattcgctgc
      841 cgctggtcgc ggtcagcgtc tgggtcggca gcggcagcaa ggacgaagcc gaaacctcgg
      901 ccggctacgc ccacttcctc gagcacatga tccagcgcgg caccgagtcg tccggtccct
      961 tcgagtacac gcgccgggcg cagcgctggg gcgggagcct gtccgtgagg gccaactacg
     1021 accgcaccgc gatcaccctg tcgggggtcc cggaaaccct cgccgagatg atcgacgccg
     1081 ccgcctcgat ggctctcagg gcgactctca aggacaagga gatcgatcag gagctcggca
     1141 ctctgatgca ggagatgcgg acgtattacg acctgccttc gtcggtggcg ctgttcgaga
     1201 cgatgcgcgc cgcgtttccg aagcatccgt accgcgtgcc gacgctgggg aacttccgca
     1261 gcgtcggcac cctgaagtcc gagccgctga ccgcctttta ccggaatctc tacgtaccca
     1321 acaacatgac cttggcggtc gccggggact tcgagccgcg cgccgccgcg gcccggatcg
     1381 aagcggcctt cggcgccgcg tcgaagagcg ccacgcttcc tccccgaccg gtgcccccgg
     1441 gcgcctttgc ggggcacacc gacgtcgaaa aaaagctcga cttcaaggag tcctggacgg
     1501 cgctggcgtt catgggcccc gggtaccgcc atccggatcg cgtcgccttc gaggcgctcg
     1561 ccacggccct cgcggatccc tcctcgccgc tgctgctcgc ttcggcgcgc gatcaggccg
     1621 gggcgctggc gcaggccagc ttctacggtc tcgaggacgc gggcctcctg tacgtcgcgc
     1681 tgaacccggc gacgccgcag ctgtcgtact ccgcggccac gtcggcgctg cgggccatcg
     1741 tcgccttccg cgccgcgggc ttctccgacg cgcaggcgcg cgatctcagg gagcggatgc
     1801 tgcgcgacga gcggcagcgc gccgcgagca tgacggagcg cgccgagcgt ctgggagagg
     1861 cgacgctgtt cggaggagtg cgctattact gggatcgccc ggtcgtcgtc tcccgcctca
     1921 ccgccgtcga actgaaccgc gtcgcggcga agtacctcgt ccccgacaac tgccgtctcg
     1981 tggtgctggt gccgaaggag acggccgact tccccgagga gacgaaggcg gagttccaca
     2041 aggtgttcga tacgctcaag gggccggagc aggaatcccc ggccggcctc gacgcgaccc
     2101 tctatccgcc gtcggaaggg gcgaaggtcg atgcggccgc gtggggcaaa gggggcggcg
     2161 cggcgccgct cggggcgccg cgccgcaccg tcctcaagaa cggcctgacg ctgctcgtcc
     2221 tcgaagatca ccgcatgccg ctcgccgccc tttcgctcca gctccgcggc ggatcgggga
     2281 gcgacgagcc cgggaaggag gggcgcgcct cgttactcct gaggttgctc gggacgcgca
     2341 ccgcccaggc cctgcgcgag gcggcgaccg gcgggggcgc gcggcgcccg ctcctgccgc
     2401 cggaggcgca ggccgggcgc gatctgctgg agctgcggct gagcgccgat ccctccgacg
     2461 tgccgctcgg gctggaagcg atcgcgaagg cggtgcgcga ggggcccgcc tccgccgatc
     2521 tcgagcgcgt ccggcaggcg gcgctccaga ccctgggccg cgccgagggt cgggccgacg
     2581 tcatcgggtt cgacctgttc cacgagaagg tctactcagg tcacgcgtac gcgcagcgtc
     2641 cggacgggac gccagcgggg ctgcaggccg tcggcctggc cgacctgcaa acgctcgccg
     2701 cgaccctcct cgatcccgcc tcctccgtcc tggcggtggc cggcgacgtg gacgcggcgg
     2761 acgtggggcg ggcggtggaa cgcctcttcg gcccctggaa gggatcgggc gcgaagcgcc
     2821 gcgactccga tgtcgccgcg gcgccgcgcg ccgcccccgc ggcaccggcg ccggcggccg
     2881 agggagagga gacgacgccc caggcgccgg tcttcgccgc cacggcgggg gcgcagccgg
     2941 gagagtacgc gcgccaggtg ggctcctccc agagcctggc gatcgtcggc gtccccggcg
     3001 tgccgctcct cgacggcgac ttcgaggcgc tccgctacct cggcgccgcg gtcacgctgt
     3061 tcggcttcga ggagatggtg ttcgagcggc gcgccgcctt ctcggtgagc agcagtccgg
     3121 aggggttcaa ggagggcggg gccctggcct tcgaggcggc cgccgcccac gcgcgacgcg
     3181 gcgaggcgca attcgagctg cagcgcctga tgcgccgcct ggcggtggag gatctcggcg
     3241 acgcggatcg ccgggatctg gcccacatcc aggccgggcg ggccgccgcg gccgcgcagg
     3301 ggacgcaggc gctcgcgagc ctcctcgcct accgcgaggc ggccgggctc ggggccgcga
     3361 actggcgcgc ccccgtcacc gtctccaccc ctcccaccgc cgcgcgcctg cgcgccctgg
     3421 cggagcggct ctttcagccg gagcggacga tccgcgtcat cgtcgggcca ccgtccccgt
     3481 agggggtctc gcatgtccga gtcgccggtc ctgtcgatcg tcctgccggt gcacaacgaa
     3541 gcggacacgc tgcgcgacag cctcgggcgg atccgggagg tgcttctccg cctcggccgc
     3601 cccttcgaga tcctggcggt ggacgatgga tcgaccgacc gcagcggcgc gatcctcgcg
     3661 gagatggcct cctccgagcc ccggctgatc ggcctcaccc tgacgcggcg cttcggcaag
     3721 gaggcggcgc tcgccgccgg gctctccgcg gcgcgcggcg aggcggtaat cctgatggac
     3781 gccgacggcc agcacccgcc cgagctgatc ccggagatgg tgcgcctctg ggaggagggg
     3841 atcgacgtcg tcgaaggggt gcgccgggat cgcggccccg aggggatccc gcaccgcctg
     3901 ctggcgcgcg ccttctacgg cctgctcggc cgcgccgtcg gccgcgacct gcagggggcg
     3961 accgactaca agcttctcga tcggcaggcg gtcgaggcgc tgctcgagtg cggcgagcgc
     4021 aaccgcttct tccgcggcct cgtcgcctgg atcggcttcc gtaccgccga ggtgccgttc
     4081 ctcgcgcccg agcggcacgg cgggacgagc aagtggtcgc tcgccgacct cctgcgctac
     4141 tccgtccgca gcctcctcgc tttcacctcc ctgccgctgc gcatcgtcgc cggggcggga
     4201 ctggtgatgg tgctgctcgg cgcgatcctg gccctgcaga cgctgtggaa ctggtcctcg
     4261 gggcacgccg tgagcggatt cacgaccgtc atcctcgtgc agctgatcct cgggggtctc
     4321 gtcctgacga gcctgggggt gatcgccgcg tatctggcgc tcctctacga ggagcagaag
     4381 gggcggccga tctacctcgt gcgccgtccg cgcggacgcc ctgcgcgtcc ggggccctcg
     4441 ccgggagcgc tcggctccta ggagcgcgcg tccggggcgc ggcttcggcg ccgggcccgg
     4501 aacggaccgc tgcgcccccc cgacttctcc tcgagccgga tcgggccgag ccgcatgccg
     4561 cggtccatcc ctttcgccat gtcgtagagc gtcagaagcg cggcggccac cgccaccatc
     4621 gcctccatct cgaccccggt gcgccagcgg gcccgcgccg tcgcttcgac cacgagcgcg
     4681 cgccgtcgcg gcgcgaaggc gatttcgacc gtcacctgat cgagcggcac gacgtgacag
     4741 agcgggatcc attcggaggt gcgcttcgcc gccgcgatgc cggcggtccg cgccagcgcc
     4801 agggcgtccc ccttcttcgc cgtgcggctc cgcagcgcgc ggaaggccgc ggcgctcatc
     4861 cggatctccc ccgaggcgcg cgcggcccgc gccgtgaccg gcttgtcgcc gacatccacc
     4921 atgcgggccg cgccgcgccg gtcgagatgg gtcagccggg gcccggcgcc gcgcatcagg
     4981 cggcccgctc cggatggccg gccgcgaggc ggtccgcggc gtcccgcaga tcgtcgggcg
     5041 tattgacgtt caggaacatc gtctcgtccc cccactcgcg gagcgcgtcg ggatcgacca
     5101 cccgcacccg catgccggcg accgccgcct gcatcgcgca ctcccccgtc gcgatcgcgc
     5161 gttcgaacgc ggcgagcgcc ggaggggcgt acgccgcggc cagaacttgc aggcggccga
     5221 gggcgcgcgg cacgaccgcg tcgtgatcct cgagccccgc gacgagcgcg ccgatcgcct
     5281 ccgccttcag gagcggcagg tcgcacgcca gcgcgaacca cgggccgggc gtgccggaca
     5341 tcccgacgcg cagcgcctgg agcggcccct cccccggatc gtccaggaag cgtgctccgt
     5401 gactctccga ctccggccgc ggcggcccga ggaccacggg cgtcccgccg gcccgtcgga
     5461 tcgtctcgac ggcgcggagc agcagtgtgc gtcctgcgat cggcagcgac gctttgtccc
     5521 gtcccatgcg ggtcgatcgc ccgcccgcga ggacgagccc ggtgaacgcc gtcgatgcga
     5581 tctggctcat gcgcgcagca tacatccccg acggcggggt gtatcatgac cgcctttccg
     5641 aggctcccat gcccgcacag cgcagcgtcg cgaagggtca aggcgaccga ccgtacggcc
     5701 gcgtcgtgcg gcgcctgctc cacgtcgggg cgcgcgacga cgtcggacgc ctggtcgatc
     5761 gcatgctgcc ggccgacatc gtggacctgt tgcgctcgct cggtcccggg gagcggcgcc
     5821 tgttcctcgg catcctgagc cagcacgacg ccgccgcctc ggtcttcgcg ctcctgccgc
     5881 cgcccctgcg gacggaggtg ctggagcagg cgaccgatga ggcgatcgcc tccctggtgg
     5941 cgcgccagcc gcccgacgaa gcggccgacc tgctcggcct gctcgaggcc tcccgcgccg
     6001 acgccgtcct gcacctgctc gacgagaaga ccgccgccaa cctcgaccgc ctgatgacct
     6061 acgggcgcga cacggctggc gggatgatga ccacccgctt catcgccctg ccgggcgcca
     6121 ccaccgtcgc ggaggcgatc gcgcgcctgc gcgccggggc acaggacgag acggtcttct
     6181 acgtctacgc cgtggacgag gcggggcggc tgcagggggt ggtgtcgctc cggcagctgg
     6241 tcctcggacg tccggagcag acgatcgaag agatcatgaa cccgcgcgtc ctcagcgtgc
     6301 ggaccgacga gccgcgcggg cgcgtcgccg atctcatcgc ccgctacaac ctcctggcgc
     6361 tgccggtcgt ggacgatcgc aacgtgctgt gcggcatcgt cacggtggac gacgcgatcg
     6421 acgcgatggc ggacgagacg acgcgcgaga tgtaccgcat ggccggcctc gacaccgagg
     6481 accgggtcac gtccccggcg tcggtctcgg tgcgccgtcg cctcccctgg atggtgatca
     6541 acctcgggac cgccatcctc gccgccacgg tcgtctcgtt cttcgaggac accatcgccc
     6601 aggtggtggc cctcgcgacg ctgatgccga tcgtcgccgg catggggggc aacggcgcca
     6661 cccagaccct cacggtgatc atccgcggga tcgccctcgg cgatctcgac ttcagcaccg
     6721 cgcgacaggc gatcctccgc gagatcaccg tcggagcggc gatcggcgcc gtcaccggga
     6781 tcctgatggc cggcgtcgcg gtgttctgga agggcaaccc ggtgctcggg ctggtgatcg
     6841 gggtggcgat ggtgatcaac ctgttcgtgg ccggcttcgc cggggcgacg gttccgatcc
     6901 tcctgaagtg gctgcggctc gatccggcgc tcggatcggg cgtcgtcgtc accaccttca
     6961 ccgactgctg cggcttcctg tcgttcctcg gtctggcgac cctgctgctc cgccacctga
     7021 cgccctgacc tccggatcac gcgactcgag ccacgcgagg atcgccgcga ccgcccccgg
     7081 ctcacggtcc aggaccgcgg cgaacgacgg catcgtcggg ccggtgccga agcgcagcgc
     7141 gcgttcgaac gcggcggggg atccgccgcc gcgccgcgca cacgcgtggc ggagatcgct
     7201 cgccctcacg accggctccg ccgtgccgtc gcctccctcg ccggcgtcgc cgagcgcctt
     7261 cccctccccg tcggcgccgt ggcacgcggc gcacccccag gcggcgtaga ggcgcgcgcc
     7321 gcgggccgcg tcggccgcgg gggcgggtac gacggtcacg tccgggatcg cgctcgctgc
     7381 gcggtcggag cgcagagcgg cgccgcgcgt cgcggtggag aaatcgcgca ccgcctgcac
     7441 caaggcccag cgatcctccg gatcgaggaa ggcgaaggag ggcatggcgg ccccggtgcc
     7501 gctcccgagc gtcaggacgc ggaagagatc ggcgtcggtg gccggagact cgagcggcac
     7561 cgatcggaag cggaacccgg cgcgcctcag gtcgcgcggc ggaatggcga ggagcgccgc
     7621 cgccggcccc ttcccgtcgc cagcggcgcc gtgacacggc acgcaccagg ccgcgtacag
     7681 ggccgcgccg cgcgcctgcc gggcctcgac ggagccgggc gaaggctccg ggaccggcgg
     7741 gtcctgggcg cggagctcgc tccagacgtc gcggcgcgcg cgtccgagcc cctgcaacca
     7801 ggcgacgagg gcgcgcccgt cgtcgttgag gcgcggcggc agggaggctc ccggaaggaa
     7861 caggtggcgc gacgccggca tgcgcgaccc cggcacgacg atctcgggag cgtagaggtg
     7921 cgccgcgtgc cagtcgtcgc tgcggcggtg cccctcgaga ccgagatcgg ggccgacgcg
     7981 gctgtcggtc ggctgcggca ggcgcggcgc cggaaggggc gtcggccctt catccaccgg
     8041 cgcgtcgaag gccgtgtgac aacggtcgca ctgctcgcgc cggtagaccg cctccccctc
     8101 ggcgtgccgc ggacccggga ggacgtccgg acgtccggat cgcggctcgt cccgcgccgg
     8161 tccggccggg gcgcaggcgg cgagaacgag cgcggcggcg ccggcgaggg tccggaggga
     8221 tcgcgcccgc gcggcgcgga tcagcgtgga ccgggctgcc ggtcgaacgc catcaggctc
     8281 aggaggaccg gcaggtagac gaccgatgcg agcagcagcc gccgcgctgc gggaagagac
     8341 ccggtgcgcg ccaggggcgc agcggccacc gcgtgcagcc cgccgaggag gagcgctccg
     8401 ccgagataga gcggacccgc cccgccccag acgacgggca ggagcgtcac cggcacgagc
     8461 gccagggcgt agaggagcgc gatccggccg gtggcggcgt tgtcgccggc gacgactgga
     8521 agcatgaggt agccgccgcg atcgtagtcg tcgcgataga ggcaggcgat ggcgagcgcg
     8581 tgcggcagct gccacacgaa caggatggcg aaaaggccca caccgaccgc ggtgacccgc
     8641 ccttccgccg ccaccgagcc gacgagcggc ggcagcgcgc ccggcagtga cccgacgagg
     8701 gtgcagagag ggctccgggt cttgagcggc gtgtagagca ggacgtaggt cagaagcgtc
     8761 agcgccacga gcccggcggc gagcgcgttc acggcgggcg ccaggaacag gatcccggcg
     8821 gtcgcgagga tgctcccgaa cagcagcgcc cgctgcggat cgaggcgtcc acccggcagc
     8881 ggccgcccgc gggtgcggcg catgcgtccg tccccgtccc gctcgaggta ctggttcagg
     8941 gcactcgccc cggccgccgc gagcgccgtc ccgaacaggg tgcgcaggag cagccccgcg
     9001 tcgagacccc gcgtcgatcc catgtagaac cccgcgagcg tcgtgagcag gaccatcgcg
     9061 gtgacccgcg gcttggtgag catcaggtag tcggcgaggc gggcgcgcgc tgcggccgcg
     9121 acctcctgcc gcgcggtgcg cgcgaccggg tctgcgtggg actcggaccg gctcatgcgc
     9181 gaacctccga ggggcggaga tcggcggcgg cggacggggc gcgccgtcgc caggcgaaga
     9241 gcgtcgtggc cagaagcgtc ccgaacagga gggctcccgt gaccacgtgc agcgtcgcgg
     9301 gcacgatcgc caggcgcgtc agcaccgcag caccgcccag gaggatctgc agcagcacga
     9361 gggtggtcgc gatccgcgcc ggcagcgcga tcgcgggatg ccggcgcagc ggcgcgcagc
     9421 ggatcgcgag tgcggccacc agccccgcca cgacgagcgc cagcacccgg tgcgcgaagt
     9481 gcacgccgat ctcgaacgac aggatcggcg gcacgatccg tccgaaagcg agcggcacat
     9541 ccgggatcgc caggccggcc ccggtgtggc gcatcagcgc cccgagcacc acctgcgcgt
     9601 acgtggccgc cgtggcgatg ccggcgagca gaccgactcc ggccggcacc ggcgaggcag
     9661 cccgcgcttc gtcgaagaag gggcgcgacg tggtcacagc cagcgcgacc acgatcgcga
     9721 agacgatctg cgccaggcag gcgtgcgcca ccgagacggc ggccggcagg cgcagcagca
     9781 ccgtcagccc gccgagcagc ccctgggcga cgaccgccgc gagcgccaga aacgccaggc
     9841 ggcgcacgcc gggtcgcggc tcgcggcgcc cgaaccagaa ggcgagcacg accgtcatca
     9901 ggccgaccgt cgccgccacc agccggtggc cgtgctcgaa gagcacccct ccccgcatct
     9961 ccgggaagac ctgaccgaag gacagtggcc agtccgggac cgccagtccg gatccggtgc
    10021 tggtcacgag ccccccggcc aggagcagca cgaccgtggc ggccaggagg ccgagggcat
    10081 agcgatgacg ccaggggtcg tacgggatcg atgcgcgcac tcgggatctt tcgtcggcag
    10141 cggggtcgcc cgggagaaac catctttata cgcgaccgac tcccccccgt caatcgccta
    10201 tgatgtcggc ccatgcgtga gattccgtcc gtgacggcca tccgggccga gggtctggcg
    10261 ttcgactacg gccgcgagcg gggcctcgac ggcgtggacc tcgaagtggc ggccggcgag
    10321 cgtttcgcca tcctcggacc gaacggcggg ggcaagacga cgctgttccg gatcctcgcg
    10381 accctcctga aaccggcgcg cggccgcgcc gcgatcttcg gccacgacgt cgccacctcc
    10441 gccgacgcgg tccgccgcgc gctcggcgtc gtgttccagg cgccggcggt cgatcgcctg
    10501 ctgacggtgc gggagaacct cgacctgcag ggggccctct acggcgtacg aggcgcggcg
    10561 cttcagcgcg agcgggaggc gcttctcgat cgcttcgggc tggcggatcg gttccgcgcg
    10621 cgggccggca ctctttccgg ggggctcctg cgccggctgg agatcgccaa ggcgctcctg
    10681 caccggccgc ggctgctgct cctcgacgag ccctccaccg gcctcgatcc gcaggcgcgc
    10741 gccgacctct ggcaggccct cgacgatcag acccgtcggg acgggacgac ggtcgcgttc
    10801 accacgcacc tgctcgacga aggggatcga tccgaccggg tggcgatcct cgatcgcggg
    10861 agggtggtcg cgctcgggac accggacgcc ctgaaggcgg agatcggacg ccaggtgctc
    10921 gtcgtcgcgg tcgaggcgcc gggggatccc ggcctgcggg ctgaggtcga gcggctcacc
    10981 ggacacccgg cgcgggaggc ggagggacgg atcctcctcg acgcgccgga cggggcgcgg
    11041 ctggtgccac tcctctccga ggccctggga ggacggatcg gcggcatcag cctgtcgcga
    11101 ccgacgctct tcgacgtctt cctgcgccgc accgggcatc gcttcgacgc cgcgccgccc
    11161 caggacttcc gcggcgccgg ggacgcggca tgagccgcgc cgcggcggcg acgctggcgc
    11221 tggcgggccg ggagacgcgg cgcttcttcc gcgagcggag ccgcgtcacg ggggcgcttc
    11281 tgcagccggt cctgttctgg gccttcttcg gcgccggcct ggcgccgtcc ttccatctcg
    11341 gcggcgggat cgcgtacggc gcgtacctgc tcccaggaat cgtcgtcctc gtgctcctgt
    11401 tcaccgcgat cttctcgacc atttcggtga tcgaggaccg gcgtgaggga ttcctgcagg
    11461 gcgtgatggt ggctccggtg ccgcgcggcg cgatcgtcgc cgggaagatc ctcggcggga
    11521 ccatcctcgc gaccgcgcag gccggcctgg cgctcctcct ggcgccgctc gccggcgtgc
    11581 ccctctccgc ggcggccttc gcggcggcgg tcgccttctg cttcgtgctc gggttcgggc
    11641 tgacgtcgct gtcgctcgcc atcgcctggc cgatgcgctc gacgcagggg ttccacgcga
    11701 tcatgacggt cttcctgatg ccgctgtggc tcctgtccgg cgccttcttc ccggccgagg
    11761 gggtgccgcg ctggctggga ctgttgatgc gcgccgaccc cctcgcctac ggcgtcgcgg
    11821 cgctccggcg gatcctcatg ccgcttcccg gcgtgccggc cgccgcggac gccggcgcca
    11881 ccgcggcggc cgtggagatc ggactcacct tcctgttcgt cgtcgccgcg ttctgcgcgg
    11941 cggtcgccgt gacccgccgc agggacggcg cgctgccagg ctgacgcgat catgcctctg
    12001 ccggccttca acgccctcat gaacgctctg tcggcgctct gcgtcgtcgc cggatggtgg
    12061 ctcatccgca acggccgccc acgggcgcac cggaccgcga tgctcctggc gcttctcgcc
    12121 tcgacgctgt tcctcggagg ctacctgacg catcacgcgc gggtcggctc ggtgccgttc
    12181 cgcggcgacg gtgcgtggcg gacgctctac ttttccatcc tgatcccgca cacgctgggg
    12241 gcggtcgcgg tcctgccgct cgtcctcgtc accgtggcgc gtgcggcgcg cggccgtttc
    12301 gaggcgcacc gtcggctggc gcgggtgacg ctgcccctgt ggctcttcgt ctcggtgacc
    12361 ggcgtcgcgg tctacgtcct cctctaccac ggcccgggcg tgaggtgagg cggaccggcc
    12421 tccaggccgc gctcgtgatg ttcgcgcgca gcgcggcccc gggacgcgtg aagacgcgcc
    12481 tcgagccgcg gctcggccgg cagggggcgc tccgcctcca cgaggcgctc gtggccgatt
    12541 cgacggacct cttgaggttg gcggcgcggc gggcccgcgc ccgcccgttc gtcgcctgga
    12601 ccggccggcc gccaaggcgc gccgccgtcc tccgccaggg gagcggcgac ctcggcgcgc
    12661 ggctccgtcg cgtcgtccgg cggctcctgt gcgcggggca ccgcgccgtc gtggtgatcg
    12721 gatcggacag ccccgccctt ccggccgcgc ggatcgtccg ggcggtgcgc ctcctcgagc
    12781 gcggcgccga tctcgtgctc gggcccgcga tcgacggcgg ctactacctc atcggcctgc
    12841 gctccgatca gccggccctg ttcgaccgca tgccgtgggg cggacgccgg gtcgccgcgc
    12901 gcacgctcga gcgcgcggcg cggctcgggc tcaggaccga gcggctccct ccgtacttcg
    12961 acgtcgatcg cccggccgat ctcgtccggc tgcgccgggc gctctcggga ccgagacggc
    13021 ggcgggcgcc gcgcaccgcc aggttgctcc ttca
//
LOCUS       DASRIQ010000044         6039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_537801, whole genome shotgun sequence.
ACCESSION   DASRIQ010000044 DASRIQ010000000
VERSION     DASRIQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537801"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFD06_01135"
     CDS             complement(<1..307)
                     /locus_tag="VFD06_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZN02167.1"
                     /translation="MMSAPIVVSFVMRSAFTLVDTVYAATLGDEAVAAVGLSIPLEFL
                     MIACWVGLSTGLTSQLSQAMGARQGARIEQVLAVTRRLVWGLVPVFTLLGLGCYVFAD
                     "
     gene            613..1713
                     /locus_tag="VFD06_01140"
     CDS             613..1713
                     /locus_tag="VFD06_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02168.1"
                     /translation="MKDELLAVLAHELRTRLNAMLGWLQLLRNNSLDVEQSEHAIEVV
                     HRNADAQVRLIDEALDASRIVSGAMEIVREPVRPGAILDAAVESLAAEARRKHIVLEK
                     EIPPDLPAILGDGQRVQQIVGNALANAVKFTPNGGHVRVRAAKGRGTVRIEVRDDGVG
                     IAPAMLPRVFDGFRLAEGGTMPERGGLGLGLAIARHLVKAHRGSIRLASGGSGRGTTV
                     TITLPAARGAADIVEVGRPDVASLPILKEVTALLVDDHEDSSEILAALLERRGAQVLV
                     ASDAGSAFETLANSTPDLLIADIAMPGIDGQELIRRVRDGGSTIPAIALSATAHPEDR
                     DRALRAGYDAYCTKPVDANELFRVIASLPGVR"
     gene            1842..3776
                     /locus_tag="VFD06_01145"
     CDS             1842..3776
                     /locus_tag="VFD06_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN02169.1"
                     /translation="MSIRLESLSKRYDGHPVVNNVTLEVTEGEFFVLLGPSGSGKSTV
                     LRLIAGLADASGGRVLLHGRDVTGLPPQSRGIGFVFQSYALFRHMSVAANVEFPLKVR
                     RTPRAERRRRRDELLELVGLAGLGGRIPGQLSGGQQQRVAIARALAHRPELLLLDEPF
                     GALDARIRADLRRTVRAIQRELKVTAVFVTHDQEEAFELADRIGVMHFGRLLETGPPS
                     ELYLRPQTEFVATFLGTANLMIGETGPDGVRLGPVRVPLATATVPGMTAPRRVQVLFR
                     PEDVAVKTTPDALGWPLLGKATVEANGFLGSFERLRLRLPRLDGVRPIAPLAPFGDDA
                     VILEATRSQHQARRFPLRPGETAFVGVRRFHALAHPGLSFLLLTDGSEGSRNPMLMAA
                     QMARMAHARVALLAYGQDGHGGERLRSLQEGISPGLASFEARHASDPLALAVGEETDR
                     RHFDLVIHAMPLRGADTLAERLLSAGDHSVLLIPLDSSGNGGGSRPAAAADQGPPRVP
                     SNAVIGVAVGEPAKEDILFAGRLIRHLGSEVTIVTVLPDQHFEAERRQAERFLKAGMR
                     TLSLLGVTSKTVLRHGAVKDEVMAEMDERNADLLVLGAPLPDERGRLNLHGVTGDLIA
                     AASDRPILIVRSHPGEPWRA"
     gene            3773..4195
                     /locus_tag="VFD06_01150"
     CDS             3773..4195
                     /locus_tag="VFD06_01150"
                     /inference="COORDINATES: protein motif:HMM:NF021566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HZN02170.1"
                     /translation="MNDPTARETLVGAIARALRGLRFGSVEITVHEGRVTLIERREKV
                     RVKDEETAPIGRPKDARSREVNPVEEDPTGRPEVPLMERADDATRNLEGSSWNDVDSD
                     DGNGWPDDGNRRRPGSPGAADRGRGAASAASRRGGDGA"
     gene            4188..5540
                     /locus_tag="VFD06_01155"
     CDS             4188..5540
                     /locus_tag="VFD06_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZN02171.1"
                     /translation="MPETPPATDADAGPASPESAGPSTQELDERLKLLERKDEVAQEQ
                     AAEKAKTAPTVTAGREGVQIRSADGSTFLRLRGYVQADGQFYTEDDERPTTNTFLLRR
                     VRPIFEGQVSRYFEFRFMPDFGAGTTVIQDAWMDWKFGSSTRLRAGKTKVPFGLERLQ
                     SALDILFAVRNAVTNIAPNRDIGLMMTGEPLDGLVNWSAGIWNGVSDGGSSDTDTGSG
                     KEGSARVFVQPFLRTSSTAFRGFGVGIAATYGQNEGTPVTPALAAYRTAGAQTFFNYR
                     SDATATAPATATGTTVASGRRKRVSPQMYWSIGRFGLLAEYARSDQEVTRAPSTTNLT
                     NEAWDAIASFLVTDDVASYKGVTPKRPFDPGSDGFGAVEIVARAGAQRQDENSFPFYA
                     NPATAAEVARDRGVGVNWYLSRNAKLMLDYIETRFAGGATGGDREDERVILNRFQVSF
                     "
     gene            5552..>6039
                     /locus_tag="VFD06_01160"
     CDS             5552..>6039
                     /locus_tag="VFD06_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN02172.1"
                     /translation="MTRPTRLLIPAAFVFTALVVATVVGGPDVQAAAGGNALLNVSYD
                     PTRELYQEVNTAFAAKWKQKSGQTVTINQSHGGSGKQARAVIDGLEADVVTLALAYDI
                     DAIARAGLLPRAWQGRLPQNSCPYTSTIVFLVRAGNPKKIKEWDDLAKPGIQVITPNP
                     KTS"
BASE COUNT          936 a         2138 c         2140 g          825 t
ORIGIN      
        1 ggtccgcgaa gacgtagcac ccgagaccga ggagcgtgaa gaccggcacc agcccccaga
       61 ccaggcggcg ggtgacggcc agcacctgct cgatgcgcgc cccctgccgc gctcccatcg
      121 cctgcgacag ctgcgacgtc agtccggtgg acagccccac ccagcaggcg atcatcagga
      181 actcgagcgg gatcgacaac ccgaccgccg ccaccgcttc gtccccgagc gtcgccgcgt
      241 agacggtgtc cacgagcgtg aacgccgagc gcatcacgaa cgacacgacg atcggcgccg
      301 acatcatgag gatggactga gagccggacg gaggggtcgg ggatgaggaa cgctgagtca
      361 aggaaaggag attctagtgc aggcgacggt ggttcgggcg cgccgcggtc gatacaatgg
      421 cttcgcgttc cctccctgga gtctcgcatg ccgatccggg atcgccgccg taaagcagcc
      481 cgcgcgctcg ggacgcgtcg cgccggtggc cgacgcagag tgcgccgccg cgccgtgcca
      541 tcgcgtcgtc gcgccgtgcg gtcgtctcgt gtcgaccgcc accgccacca cggggcggac
      601 gccgccggcc ggatgaagga cgagctgctc gcggtgctcg cccacgagct gcggacgcgg
      661 ctgaacgcga tgctcggatg gttgcagctc ctgcgcaaca acagtctcga cgtcgagcag
      721 tccgagcacg cgatcgaggt ggtgcaccgc aacgcggacg cccaggtgcg gctcatcgac
      781 gaggccctgg acgcctcgcg catcgtcagc ggcgcgatgg agatcgtccg ggagccggtg
      841 cggccgggag cgatcctcga cgcggccgtc gagtcgctcg ccgcggaggc gcgccggaag
      901 cacatcgtcc tcgagaagga gatcccgccc gacctgccag cgatcctggg cgacgggcag
      961 cgcgtgcagc agatcgtcgg gaacgccctc gccaacgccg tgaagttcac tccgaacggc
     1021 ggccacgtgc gggtccgcgc ggcgaagggg cgcggcacgg tccgcatcga ggtgcgggac
     1081 gacggcgtcg gcatcgcgcc tgcgatgctc ccgcgcgtct tcgacggctt ccggctggcg
     1141 gaaggcggga ccatgccgga gcgcggcggc ctcggtctgg gcctggcgat cgcccggcac
     1201 ctcgtgaagg cccaccgtgg atcgatccgg ctggcgagcg gcggttcggg gcggggaacc
     1261 accgtgacca tcaccttgcc tgcggcccgc ggggcggcgg acatcgtcga ggtcgggcgt
     1321 ccggacgtcg cgagcctgcc gatcctgaag gaggtgacgg cgctcctggt ggacgatcac
     1381 gaggactcga gcgagatcct cgcggccctc ctggagcggc gcggcgcgca ggtcctcgtg
     1441 gcgtcggacg cagggtcggc gttcgagacg ctggcgaaca gcacccccga tctcctcatc
     1501 gccgacatcg ccatgccggg catcgacggc caggaactga tccgccgggt ccgcgacggc
     1561 ggcagcacca tcccggccat cgccctcagc gccaccgcgc accccgagga tcgcgaccgg
     1621 gcgctccggg caggatacga cgcctactgc accaagccgg tggacgcgaa cgagctgttc
     1681 cgcgtcatcg cgtcgctgcc tggcgtccga tagccttctc tgcggccggt gccgtgcgaa
     1741 ttcaaaccgt tttgtgacca cggttcgact tcatctgtga ccgctgggaa gtagattcca
     1801 gcgtcggctt ccgccgccct ggcggggccg cggtcaccga catgtcgatc cggctcgaat
     1861 cgctctcgaa gcgctacgac gggcatcccg tcgtcaacaa cgtcacgctc gaggtgacgg
     1921 agggggagtt cttcgtcctc ctcggcccct ccgggagcgg caagagcacg gtgctgcggc
     1981 tcatcgccgg gctcgccgac gccagcggcg ggcgggtgct cctgcacggg cgcgacgtca
     2041 ccggcctgcc gccgcagagt cgcggcatcg gcttcgtctt ccagtcgtac gcgctgttcc
     2101 gccacatgag tgtggcggcg aacgtcgagt tccctctcaa ggtgcggcgg acgccgcgcg
     2161 ccgagcggcg ccgccgccgc gacgagctgc tggagctggt ggggctcgcc ggtctcggcg
     2221 ggcggatccc cgggcagctt tccggcgggc agcagcagcg cgtggcgatc gcccgggcgc
     2281 tggcgcatcg gccggagctg ctcctgctcg acgagccgtt cggagcgctc gacgcgcgca
     2341 tccgcgccga cctgcgccgc acggtgcgcg ccatccagcg cgagctgaag gtcaccgcgg
     2401 tcttcgtcac ccacgaccag gaggaggcgt tcgaactggc cgaccggatc ggagtcatgc
     2461 acttcggccg gctgctcgag accggcccac cgtcggagct gtacctgcgt ccgcagaccg
     2521 agttcgtcgc gaccttcctc gggaccgcca acctcatgat cggcgagacc ggcccggacg
     2581 gcgtccgcct cggaccggtg cgcgtgccgc tggcgaccgc caccgtcccc ggcatgacgg
     2641 cgccgaggcg cgtgcaggtg ctgttccgcc ccgaggacgt cgcggtgaag accaccccgg
     2701 acgccctcgg ctggccgctc ctcggcaagg cgacggtcga ggcgaacggc ttcctgggct
     2761 cgttcgagcg gctgcgactc cggctgccgc gcctcgacgg ggtgcgcccg atcgccccgc
     2821 tcgcgccgtt cggcgacgac gcggtgatcc tcgaggcgac ccgctcgcag caccaggcgc
     2881 gccgcttccc gctgcgcccc ggcgagacgg cgttcgtcgg cgtgcgccgc ttccacgccc
     2941 tggcccaccc gggtctgtcg ttcctcctgc tcaccgacgg ttcggagggg agccgcaacc
     3001 cgatgttgat ggcggcgcag atggcccgca tggcgcacgc gcgcgtcgcc cttctcgcct
     3061 acggccagga cgggcacggc ggcgagcgcc tgcggagcct gcaggagggg atctccccgg
     3121 ggctcgcctc gttcgaggcg cggcacgcgt ccgatccgct cgcgctcgca gtcggcgagg
     3181 aaaccgaccg gcggcacttc gacctggtga tccatgccat gccgctgcgc ggcgccgaca
     3241 ccctggcgga gcggctgctc agtgccgggg accacagcgt cctgctcatc cccctcgatt
     3301 cgagcggcaa cggcggcggc agccggccgg ccgcggccgc cgatcagggg ccgccgcgcg
     3361 tgccgtccaa tgcggtgatc ggggtcgcgg tcggcgagcc ggccaaggag gacatcctgt
     3421 tcgccggacg tctcatccgc cacctcggaa gcgaagtcac catcgtgacg gtcctccccg
     3481 accagcactt cgaagcggag cgccggcagg cggagcggtt cctgaaggcc ggcatgcgga
     3541 cgctgtcgct gctcggcgtc acgtcgaaga ccgtgctccg gcacggcgcg gtgaaggacg
     3601 aggtgatggc ggagatggac gagaggaacg cggatcttct ggtgctcggc gctccgctcc
     3661 cggacgagcg aggccggctc aacctgcacg gcgtcaccgg cgatctcatc gcggccgctt
     3721 ccgaccgccc gatcctgatc gtccgatcgc accccgggga gccgtggcgg gcatgaacga
     3781 cccgacggcc cgcgagacgc tcgtgggcgc catcgcgcgc gccttgcgcg gcctgcgctt
     3841 cgggtcggtg gagatcaccg tgcacgaggg gcgcgtgacg ctcatcgagc gccgggaaaa
     3901 agtacgagtg aaggacgagg agaccgctcc gatcggacga ccgaaggacg cgcgctcccg
     3961 cgaggtgaac ccagtcgagg aggacccgac cggacgaccg gaggttccgc tgatggagcg
     4021 cgccgacgat gcgacgcgga acctggaagg ttcttcttgg aacgatgttg attctgacga
     4081 tgggaacggg tggcctgacg acggcaatcg ccgccgaccc ggatcccccg gcgccgccga
     4141 ccgcggaaga ggagccgcct ccgccgcctc ccgacgagga ggagacggtg cctgagacgc
     4201 cacccgcgac ggatgccgat gccgggccgg cgagccccga atcggccggt ccgtccaccc
     4261 aggagctgga cgagcgcctg aagctcctgg agcggaagga cgaggtggcg caggagcagg
     4321 cggcggagaa ggcgaagacg gcgccgaccg tcaccgccgg ccgcgaaggg gtgcagatcc
     4381 ggtcggccga cggctcgacg ttcctgcggc tgcgcgggta tgtccaggcg gacggtcagt
     4441 tctacaccga ggacgacgag cggccgacga cgaacacctt cctcctgcgc cgcgtccggc
     4501 cgatcttcga agggcaggtc tccaggtact tcgagttccg cttcatgccc gacttcggcg
     4561 ccgggacgac ggtcatccag gacgcctgga tggactggaa gttcggatcc tcgacgcgcc
     4621 tgcgggccgg caagaccaag gtcccgttcg gattggagcg gctgcagtcg gcgctcgaca
     4681 tcctgttcgc ggtgcgcaac gcggtcacca acatcgcgcc gaaccgcgac atcgggctga
     4741 tgatgaccgg cgagccgctc gacggcctcg tcaactggtc ggccggcatc tggaacggcg
     4801 tgtccgacgg cggcagcagc gacaccgata ccggcagcgg caaggaggga tcggcgcgcg
     4861 tcttcgtgca gccgttcctg cgcacctcgt ccacggcatt ccgcggtttc ggcgtgggaa
     4921 tcgcggcgac gtacggccag aacgagggga cgccggtgac gccggccctt gcggcctacc
     4981 gcacggcggg agcgcagacg ttcttcaact accgcagcga tgcgaccgcg accgctccgg
     5041 ccacagccac cgggacgacg gttgcgagcg gccggcgcaa gcgcgtctcc ccacagatgt
     5101 attggtcgat cggacgtttc ggcctgctcg ccgaatatgc ccgctccgac caggaggtga
     5161 cgcgcgcccc gtccacgacc aacctgacga acgaggcatg ggacgcgatc gcatcgttcc
     5221 tcgtcaccga cgacgtggcg tcgtacaagg gtgtgacgcc gaaaaggccc ttcgatccgg
     5281 gttcggacgg cttcggcgcg gtcgagatcg tcgcccgcgc cggggcccag cgccaggacg
     5341 agaactcgtt cccgttttac gccaaccctg cgaccgcggc cgaggtggcg cgggatcgcg
     5401 gcgtcggggt gaactggtac ctgagccgca acgccaaact gatgctcgac tacatcgaaa
     5461 cgcgcttcgc gggcggcgcc acgggcggcg accgcgagga cgagcgggtg atcctgaacc
     5521 gcttccaggt ttcgttctga gggagacaga catgaccagg ccgacacgct tgctgattcc
     5581 tgccgccttc gtcttcaccg ctctcgtcgt cgccacagtc gtcggcggac cggacgtcca
     5641 ggccgccgcc ggcgggaacg cgctcctgaa cgtctcgtac gacccgaccc gcgagctgta
     5701 ccaggaggtc aacaccgcct tcgctgcgaa gtggaagcag aagagcggcc agacggtcac
     5761 catcaaccag tcgcacggcg gctccggcaa gcaggcgcgc gccgtcatcg acgggctcga
     5821 agcggacgtc gtgacgctgg cgctggcgta cgacatcgac gccatcgcgc gcgccggcct
     5881 cctgccgcgc gcctggcagg ggagactgcc gcagaactcc tgcccttaca cctcgaccat
     5941 cgtgttcctc gtccgggccg ggaacccgaa gaagatcaag gagtgggacg atctggccaa
     6001 gcctggcatc caggtgatca ctccgaaccc gaagacctc
//
LOCUS       DASRIQ010000045         4160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1067319, whole genome shotgun sequence.
ACCESSION   DASRIQ010000045 DASRIQ010000000
VERSION     DASRIQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067319"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFD06_01165"
     CDS             complement(<1..248)
                     /locus_tag="VFD06_01165"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZN02173.1"
                     /translation="MSGLHFGNPIGGVLLGLLLLALLWRVARARGRHASLVFSVTRPF
                     RTIPAGRGVRLRHVPTGLRMLGLALLVIAFARPQTWFS"
     gene            complement(245..1168)
                     /locus_tag="VFD06_01170"
     CDS             complement(245..1168)
                     /locus_tag="VFD06_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02174.1"
                     /translation="MSVQVSARADHVEVTVGDPITVTFTVRHPEGTTVAAFDADRALG
                     GLEQLAAIDTAPRRLPDGTVEEGRVVTFAAYKPGPGEIPAVRVVLRDAAGKEIEGTTA
                     PIPFRVASVLKEGESEPADLKRPVEMKELLVWPWFVLGALLLAVAAFLWWRRLRQRRP
                     AAPVPVIPAAPPRPEHDIAYEELQRLLASGLLERGEVKKFYIALAEIIRRYLGRRFDF
                     DAFECTTWEILEAVRGARLPIPTQTLLAELLGLCDLVKFAKHAPGREETQGSVERAYR
                     LVDETRRVAPPPLPSEAAAPPPGPVASRGVA"
     gene            complement(1165..2046)
                     /locus_tag="VFD06_01175"
     CDS             complement(1165..2046)
                     /locus_tag="VFD06_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN02175.1"
                     /translation="MLPAEILRKVRRIEIRTRRLVNESLAGEYHSVFKGRGMEFSEVR
                     EYQWGDDMRTIDWNVTSRMGHPYVKKHVEERELTVLLLVDVSASGDFGTRRQFKREIQ
                     AEICALLAFSAIQNSDRVGLIAFTDRIETFLRPRKGKDHVLRVIREVLYFSPQGRTTD
                     FKMALEHLNRTITKRGVVFIISDFLAGDFERPLRVAARKHDVVAITVTDPREEELPPI
                     GLLELEDAETGDRVLVDSRDKRTRAAFGRWAAERRQARESLFRSIGIDALDLRTDRPY
                     DLPLVRFFHRRARRLAR"
     gene            complement(2105..3091)
                     /locus_tag="VFD06_01180"
     CDS             complement(2105..3091)
                     /locus_tag="VFD06_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN02176.1"
                     /translation="MEQDVRRLNDLVAREAVFVEQVLGEIRKVIVGQKALLDRLLIGL
                     LADGHLLLEGVPGLAKTLSVKTLADIIDLKFQRIQFTPDLLPADLVGTMIFNQKDGMF
                     VPRKGPVFANIVLADEINRAPAKVQSALLESMQERQVTFGDTTYPLDRPFLVLATQNP
                     IEQEGTYPLPEAQVDRFMLKIKVDYPTKTEEREILERMSTLQDLRVRKVVNGADILKA
                     RQVVAQVYVDNRLKDYIVDMIMATRRPDEYKLDVKGLIQFGASPRATVFLTLASKAHA
                     FLRGRGYVTPEDVKSVAFDVLRHRIILSYEAEAEDVTTEEIIQRVLDTIEVP"
     gene            complement(3237..>4160)
                     /locus_tag="VFD06_01185"
     CDS             complement(3237..>4160)
                     /locus_tag="VFD06_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02177.1"
                     /translation="AYVSAGIRSGGQVVAGENNWGTEVFGSDVDWPFIRAWNVERGVF
                     FSEAEVKAGTKVAVLGATVVRELFPDSDPVGSVIRIKNVPFKVIGVLERKGGSVMGSD
                     QDDQVVVPYTTVMKRLTGAIRLGIVQVAAVSPDRIGQAMDEIRALLRQRHRLTPSQED
                     DFNMRSQEEIAATAGQTARTFSILLASVAAISLLVGGIGIMNIMLVSVTERTREIGLR
                     MAVGAKGRHVLGQFLLESVALSVVGGAIGVGLGIGASLLIARFAGWPVSIDPSAVAVA
                     FLFAGFVGVFFGFYPARKAARLDPIEALRYE"
BASE COUNT          632 a         1456 c         1466 g          606 t
ORIGIN      
        1 tcgctgaacc aggtctgcgg ccgggcgaag gcgatcacca gcagggcgag tccgagcatc
       61 cggaggcccg tcggcacgtg gcgcaggcgc accccgcggc cggccgggat cgtccggaac
      121 ggccgggtca cggagaagac caggctcgcg tgccggccgc gcgcccgcgc cacccgccag
      181 aggagggcga ggagcaggag cccgaggagg acgccgccga tcgggttgcc gaagtgcagg
      241 ccgctcatgc gaccccccgc gacgccaccg gaccgggcgg cggagccgcc gcctccgacg
      301 gaagcggcgg cggcgcgacc cggcgggtct cgtccaccag gcgataggcg cgctcgacgc
      361 tcccctgcgt ctcctcgcgc cccggggcgt gcttggcgaa cttgacgaga tcgcacagtc
      421 ccaggagctc ggcgagaagc gtctgggtgg ggatcggcag ccgggcgccg cgcaccgctt
      481 cgaggatctc ccaggtcgtg cactcgaagg cgtcgaaatc gaaccgccgc ccgagatagc
      541 gccggatgat ctccgcgagc gcgatgtaga acttcttcac ctcgccccgc tccaggagcc
      601 ccgaggcgag caggcgctgc agctcctcgt aggcgatgtc gtgctccggg cggggcggcg
      661 ccgccggaat caccggcacg ggggcggccg gacggcgctg gcggagacga cgccaccaca
      721 ggaacgccgc caccgcgagg agcagcgcgc cgaggacgaa ccacggccag acgagcagct
      781 ccttcatctc caccggacgc ttcaggtcgg ccggctcgct ctccccttcc ttcagcaccg
      841 aggcgacgcg gaaggggatc ggggccgtgg tcccctcgat ctccttcccg gccgcgtcgc
      901 gcagcaccac ccggaccgcc gggatctcgc cgggacccgg cttgtaggcg gcgaaggtga
      961 cgacgcgccc ctcctccacc gtcccgtccg ggagccggcg gggggcggtg tcgatcgcgg
     1021 cgagctgttc gagcccgccc agcgcgcggt cggcgtcgaa ggcggcgacc gtcgtcccct
     1081 ccgggtgccg caccgtgaac gtgaccgtga tcggatcgcc gacggtcacc tcgacgtggt
     1141 cggcgcgcgc cgagacctgg acgctcatcg cgccagcctc cgggcgcggc ggtggaagaa
     1201 gcgcaccagc ggcaggtcgt agggacggtc ggtgcgcaga tcgagggcgt cgatgccgat
     1261 ggagcggaag aggctctcgc gggcctgccg ccgctccgcc gcccagcgac cgaaggcggc
     1321 ccgcgtccgc ttgtcccgcg agtccaccag cacccggtcg ccggtctcgg cgtcctccag
     1381 ctccaggagc ccgatgggcg gaagctcttc ctcgcgcgga tcggtgacgg tgatcgccac
     1441 gacgtcgtgc ttgcgcgccg cgacccggag cggccgctcg aagtcgccgg ccaggaagtc
     1501 ggagatgatg aagacgacgc cgcgcttggt gatggtgcgg ttcaggtgct cgagcgccat
     1561 cttgaagtcg gtcgtgcgcc cttgcgggct gaagtagagc acctcgcgga tgacgcgcag
     1621 cacgtggtcc ttccccttgc gcggccgcag gaacgtctcg atccggtcgg tgaaggcgat
     1681 gagcccgacc cggtcgctgt tctggatcgc cgagaaggcg agcagcgcgc agatctccgc
     1741 ctgaatctca cgcttgaact ggcggcgcgt cccgaagtcg ccggacgccg acacatccac
     1801 gagcaagaga acggtgagct cccgctcctc gacgtgcttc ttgacgtacg ggtgccccat
     1861 gcgggaggtg acgttccagt cgatcgtgcg catgtcgtcg ccccactggt actcgcgcac
     1921 ctcgctgaac tccatgccgc gacccttgaa gacggagtgg tactcccccg ccagcgactc
     1981 gttgacgagc cggcgggtgc ggatctcgat gcgtcggacc ttcctgagga tttccgcggg
     2041 cagcatgggt gggccggttc cccccggcgc cgcccccgcg ggcggcgccg cgatggcggg
     2101 cgttctaggg gacttcgatg gtatcgagca ccctctggat gatctcttcc gtcgtcacgt
     2161 cctcggcctc ggcctcgtac gacaggatga tgcggtgacg caggacgtcg aacgccaccg
     2221 acttcacgtc ctcgggggtg acgtagccgc ggccgcgcag gaaggcgtgc gccttcgacg
     2281 ccagcgtcag gaagacggtg gcgcgcggcg aggccccgaa ctgaatcagt cccttcacgt
     2341 cgagcttgta ctcgtccggc cggcgcgtcg ccatgatcat gtcgacgatg tagtccttga
     2401 ggcggttgtc gacgtagacc tgcgccacca cctggcgcgc cttgaggatg tcggccccgt
     2461 tgacgacctt gcgcacgcgc aaatcctgga gggtgctcat ccgctcgaga atctcgcgct
     2521 cctcggtctt cgtcgggtag tcgaccttga tcttcagcat gaagcggtcg acctgcgcct
     2581 ccggcagcgg gtaggtccct tcctgctcga tcgggttctg cgtcgccagc accaggaacg
     2641 gccgatcgag gggataggtg gtgtcgccga aggtgacctg ccgctcctgc atcgactcga
     2701 gcagcgccga ctgcaccttc gccggggcgc ggttgatctc gtccgccagg acgatgttgg
     2761 cgaacaccgg ccccttgcgc ggcacgaaca tgccgtcctt ctggttgaag atcatcgtgc
     2821 cgacgaggtc ggccggcagc aggtcggggg tgaactggat gcgctggaac ttgagatcga
     2881 tgatgtcggc cagcgtcttg accgacagcg tcttcgccag ccccggcacc ccttcgagga
     2941 ggaggtgccc gtccgccagg aggccgatca gcagccggtc gaggagcgcc ttctgcccga
     3001 cgatgacctt gcggatctcg ccgaggacct gctcgacgaa aaccgcctct cttgccacga
     3061 ggtcgttgag gcgccggacg tcctgttcca tgacgggggt cgcttccatt cgggatcctc
     3121 gcgaaaaggg gcggaattgt agaccacggt cccgcccatg tcgaggatcc ggcgctggag
     3181 cgggtgcccc gcgcccctgc cgtcgctgcg gctcgccgcg cctggggggg gccgggctac
     3241 tcgtagcgga gggcttcgat ggggtcgagc cgggcggctt tgcgggcggg gtagaagccg
     3301 aagaagacgc cgacgaagcc ggcgaacagg aacgccaccg cgacggccga cgggtcgatc
     3361 gacaccggcc agcccgcgaa gcgcgcgatg aggagcgagg cgccgatgcc gagcccgacg
     3421 ccgatcgctc ccccgacgac cgacagcgcc accgattcga ggaggaactg gcccaggacg
     3481 tggcgcccct tggcgccgac cgccatgcgc aggccgatct cgcgggtccg ctcggtgacc
     3541 gacaccagca tgatgttcat gatgccgatg cccccgacca gcagggagat ggcggcgacg
     3601 gaggccagga ggatcgagaa ggtccgggcg gtctgccccg cggtcgcggc gatctcctcc
     3661 tgcgagcgca tgttgaagtc gtcctcctgc gacggggtca gccggtgccg ctggcgcagg
     3721 agcgcccgga tctcgtccat cgcctggccg atgcggtcgg gggagacggc ggccacctgg
     3781 acgatgccga ggcggatggc gccggtgagc cgcttcatga cggtggtgta gggaacgacg
     3841 acctggtcgt cctggtccga ccccatgacg ctccccccct tgcgctccag gaccccgatc
     3901 accttgaacg gaacgttctt gatccggatg accgagccga ccgggtcgga gtcggggaag
     3961 agctcgcgca ccaccgtcgc cccgagcacc gccaccttgg tgccggcctt cacctccgcc
     4021 tcgctgaaga acacgccccg ctccacgttc caggcgcgga tgaacggcca gtccacgtcg
     4081 gagccgaaca cctcggtccc ccagttgttc tcccccgcca ccacctggcc cccggagcgg
     4141 atgccggccg agacgtaggc
//
LOCUS       DASRIQ010000046         4951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3668153, whole genome shotgun sequence.
ACCESSION   DASRIQ010000046 DASRIQ010000000
VERSION     DASRIQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3668153"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1044)
                     /locus_tag="VFD06_01190"
     CDS             complement(34..1044)
                     /locus_tag="VFD06_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HZN02178.1"
                     /translation="MRHIRLHHDWRASLTAAAWAILLLASATTPALANGGDIRDIEVG
                     VYAGSMSPDSYDSLDPDNGTFWGLRGGYFFTPRWSVEGTWQDFSTEGDVAGASPDISL
                     KSLRANGLMNFRAKKKFRWFLTIGLGKEKIESDDLDVSEKALGWNYGGGARWFFGKSK
                     FWGLRADARWVTVDPGGDIDGNQTNFEWGGGLMFTFGGGTPPDTDGDKVNDKADKCAG
                     TPKGATVDAAGCPKDTDGDRVYDGLDKCASTPAGWTVDPTGCPADKDGDGVADKVDTC
                     PDTPKEAKVDVLGCPIEDADKDGIWDKVDRCPDTGHDLKVDPVGCPVDADKDGNWDKK
                     NP"
     gene            complement(1054..1944)
                     /locus_tag="VFD06_01195"
     CDS             complement(1054..1944)
                     /locus_tag="VFD06_01195"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZN02179.1"
                     /translation="MPVRRLALLVLLLVPTAVAAQDKPAEPKKLGWKDTAELGYVATS
                     GNSDSSTLGLKNTLTYDWEEARFEMKLGAIRVESGQGFVALPTATGFRREDSDPELSA
                     ESYYLNGRYDHNITERFFWFAGAGWDRNTFAGIENRYAAFGGVGNIWSDSETIKWRTD
                     YAATATKQEDVVEQPDFDDTFLGLRLSSTFLYKWSESGSYGNDTIVDENVDDTDDWRV
                     NMTNWVGVNMSTHLALKVSLQWLYDHQPAFVTADVFSADPDEGGVPVDVNPADGQTDQ
                     AAVEADTLDTIFTTALVIKY"
     gene            complement(1974..2396)
                     /gene="mscL"
                     /locus_tag="VFD06_01200"
     CDS             complement(1974..2396)
                     /gene="mscL"
                     /locus_tag="VFD06_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZN02180.1"
                     /translation="MKEFKEFLLKTNALALAIGVIIGGAIGKVVTSLVNDLLMPPIGL
                     LLGNVDFSNLFLNLSSNHYATIDEAKKAGAPTLNYGLFINSIIDFVIIAFCIFIISKI
                     AMKPAPAPPAPANKECPQCCETILKAAKKCRYCGSPQA"
     gene            2502..4427
                     /locus_tag="VFD06_01205"
     CDS             2502..4427
                     /locus_tag="VFD06_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02181.1"
                     /translation="MLRRTSLLVFGVAFAVRMAYVAGVRHLLWFPVPLVDGANYFRLA
                     HRIASGDLLGGGETFWQPPLYPYFLAVLFRLFGERMIVVVTAQAALGALTCVLVAWIG
                     ARLWGWKAGVVASLLAAFYGPLVHFDAQPLVSVLHTALVTGGLLALLRAAGIDAARSA
                     SDASGGDAARPSGTARSWALAGLLWGLAAIATPNLLLVVPAAAAWILLRRLRARPLLP
                     WLAGLALPVALVAGRNLFVAGEPVLVSSNGGINLHIGNNPDYDRTVRIRPGGEFERLA
                     QEPENLGILGAAARSRWFTQRAVSFWTGYPGPAARLFARKARDLVAGREIPRNENLYE
                     YRSASTVLSLLVWRFGVSFPFGVVAPLALAGVFLALRERGRDGSILLLVTAAAYAVSI
                     LLFFPTDRYRLPLVPIAVLFAARLLVDLRALRRPAVVLALAAGFVLFNLDAATATESY
                     PEEEALNRAYALRALGRADEAVAEYERAFALNPGRLDAPNSLAVLAAERGDWDRAVVR
                     YREALAIDPGFVDLRRSLGEALLALGRREEARQEWTIAAGLAPAAGLVLADLALLDLE
                     EGALQPAEDRALAAVRARPDLPETHMALGIVSRALRRRDRAMVSFETAARLFPAGSTG
                     RARATEWLDRMRRRGAP"
     gene            4457..>4951
                     /locus_tag="VFD06_01210"
     CDS             4457..>4951
                     /locus_tag="VFD06_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZN02182.1"
                     /translation="MKPIARATPLTTPARRRLAGRVLPAAAAAALAVLLGAAPAGAQT
                     TIDYDGNVDFTKYKTYSWGRNTSSGNPAADERIIAEIEAHLTAAGWKQAPPDEGEVVV
                     AEHATVTNDVSVDTFYTGWGTGWGWTGFGPGAGVGTTVSQNLRSGTLVVDMFDATTKK
                     LIWRG"
BASE COUNT          639 a         1698 c         1736 g          878 t
ORIGIN      
        1 tgcccttttt cccattccgg cctgggggcc gctctatggg ttcttcttgt cccagttgcc
       61 gtctttgtcg gcgtcaacag ggcatcccac cggatcgacc ttgagatcgt gcccggtgtc
      121 ggggcagcgg tcgaccttgt cccagatgcc gtccttgtcc gcatcctcga tggggcagcc
      181 gaggacgtcg accttggctt ccttgggggt gtccgggcag gtgtccacct tgtcggcgac
      241 gccgtcgccg tccttgtcgg cggggcagcc ggtggggtcg accgtccacc cggcgggggt
      301 ggaggcgcac ttgtcgagac cgtcatagac ccggtctccg tcggtgtcct tggggcagcc
      361 tgcggcgtcc acggtcgccc ccttcggggt cccggcgcac ttgtccgcct tgtcattgac
      421 cttgtcgccg tcggtgtccg gaggcgtccc gccgccgaac gtgaacatca gcccgccgcc
      481 ccactcgaag ttcgtctggt tgccgtcgat gtctcccccc ggatcgaccg tcacccagcg
      541 ggcgtccgcg cgcagccccc agaacttcga ctttccgaag aaccagcggg ccccgccacc
      601 gtagttccat ccgagcgcct tctcggagac gtcgaggtcg tcggattcga tcttctcctt
      661 cccgaggccg atggtcagga accaccggaa cttcttcttg gcgcggaaat tcatcagtcc
      721 gttggcgcgg agcgacttca ggctgatgtc gggcgatgcc ccggcgacgt ccccctcggt
      781 cgagaagtcc tgccaggttc cctcgacgct ccacctcggc gtgaagaagt agccgccgcg
      841 cagcccccag aaggtgccgt tgtcgggatc gagcgagtcg tacgagtcgg gggacatgct
      901 gccggcgtac acgcccacct cgatgtcccg gatgtcgccg ccgttggcga gggccggggt
      961 cgtcgccgac gccagcagca ggatcgccca cgccgccgcc gtgagcgacg cgcgccagtc
     1021 gtgatggagc cgaatgtgtc gcatggttcc ctcctaatac ttgatgacga gggcggtggt
     1081 gaagatggtg tcgagcgtat ccgcctcgac ggcggcctga tccgtctgac cgtcggcggg
     1141 gttgacgtcc accggcacgc ccccctcgtc cggatccgcc gagaacacgt cggcggtgac
     1201 aaatgccggc tgatggtcgt acagccactg caggctgacc ttgagcgcca ggtgcgtgct
     1261 catgttgacc cccacccagt tggtcatgtt gacccgccag tcgtcggtgt cgtcgacgtt
     1321 ctcgtccacg atggtgtcgt tgccgtacga gccgctctcc gaccacttgt agagaaacgt
     1381 cgaggacagg cgcagcccga ggaaggtgtc gtcgaagtcc ggctgctcga cgacgtcctc
     1441 ctgcttggtc gcggtggccg catagtcggt ccgccacttg atcgtctcgc tgtccgacca
     1501 gatgttgccg acgccgccga aggcggcgta gcgattctcg atgcccgcga acgtgttgcg
     1561 gtcccagccg gcgccggcga accagaagaa gcgctcggtg atgttgtgat cgtaccgtcc
     1621 gttaaggtag taggactcgg cgctcagctc ggggtcgctg tcctcgcgtc ggaaaccggt
     1681 cgcggtggga agggcgacga acccctggcc cgattccacg cggatggcgc ccagcttcat
     1741 ctcgaagcgc gcctcctccc agtcgtaggt cagcgtgttc ttgaggccga gcgtgctgct
     1801 gtcgctgttt cccgaggtcg cgacgtagcc gagctcggcg gtgtccttcc agccgagctt
     1861 cttcggctcg gccggcttgt cctgggcggc gacggccgtc ggcacgagca ggaggacgag
     1921 gagggcgagg cgtcgaacgg gcatgcaggt ctccggtgcg cgcggggcgc ggatcacgct
     1981 tgcgggctgc cgcagtagcg gcacttcttg gccgccttga ggatcgtctc gcagcactgc
     2041 gggcattcct tgttggccgg ggcggggggc gccggcgccg gcttcatggc gatcttgctg
     2101 atgatgaaga tgcagaaggc gatgatgacg aagtcgatga tcgagttgat gaacaggccg
     2161 tagttcagcg tcggcgcgcc ggccttcttg gcttcgtcga tggtcgcgta gtggttgctg
     2221 gagagattca ggaagaggtt ggagaagtcc acgttcccga gcagaagacc gatcggcggc
     2281 atcaggaggt cgttgacgag cgacgtgacg accttgccga tcgccccgcc gatgatgact
     2341 ccgatcgcga gggcgagcgc attggtcttg agcaggaatt ctttgaactc cttcatgcaa
     2401 ccccctctcc tggccggcgg caccgtcgcc gcgggaaaag attcgtcttc ggttgtccgc
     2461 gagggggcgg aggatagcac aggaggatac actcggaccc gatgctgcgg cggacctcgc
     2521 tcctcgtttt cggcgtggcg ttcgccgtcc gcatggccta tgtcgcgggc gtccgccacc
     2581 tgctctggtt cccggtcccg ctcgtcgacg gcgccaatta tttccggctg gcgcaccgca
     2641 tcgcgagcgg agatcttctc ggcggcggcg agacgttctg gcagccgccg ctgtatccgt
     2701 acttcctggc ggtcctcttc cggcttttcg gcgagcggat gatcgtggtc gtgacggcgc
     2761 aggccgcgct cggcgcgctg acctgcgtgc tggtggcgtg gatcggcgcg aggttgtggg
     2821 gatggaaggc gggtgttgtc gcttcgctcc tcgccgcctt ctacggaccg ctcgtccact
     2881 tcgacgcgca gccgctcgtg tcggtgctgc atacggcgct cgtgaccggc ggcctgctgg
     2941 cgcttttgcg cgccgccggc atcgacgcgg cgcgctccgc gtcggacgcc tccggcggcg
     3001 atgcagcgcg cccctccggg accgcacgct cctgggcgct ggcggggctt ctgtgggggc
     3061 tcgcggcgat cgccacgccg aacctcctgc tcgtcgttcc cgccgcggcg gcctggatcc
     3121 tgctgcgccg cctccgggcg cggcctctcc tgccgtggct cgcggggctg gcgctgccgg
     3181 tcgcgctggt ggcggggcgc aacctcttcg tggcggggga gccggtgctg gtgtcgagca
     3241 acggcggcat caacctgcac atcggcaaca acccggacta cgaccggacg gtgcgcatcc
     3301 gccccggcgg ggagttcgag cgcctggcgc aggagccgga gaacctcggc atcctcggag
     3361 ccgccgcccg ctcccgctgg ttcacgcaac gcgccgtgtc cttctggacc gggtatcccg
     3421 gccccgcggc gcggctcttc gcgcgcaagg cgcgcgacct cgtcgccggc cgcgagatcc
     3481 cgcgcaacga gaacctgtac gagtaccgga gcgcctcgac ggtgctgtcg ctcctggtgt
     3541 ggcgcttcgg cgtgtccttc cccttcggtg tcgtcgcgcc tctggcgctc gcgggggtct
     3601 tcctggcgct gcgggaacgc ggccgcgatg ggtcgatcct ccttctcgtt accgcggccg
     3661 cgtacgccgt ttcgatcctg ctgttctttc cgaccgatcg ctatcgcctg ccgctcgtgc
     3721 cgatcgccgt cctgttcgcg gcccggctgc tcgtcgatct ccgggcgctg cggcggccgg
     3781 cggtcgtcct cgcgctcgcg gcagggttcg tcctcttcaa cctggacgcc gcgaccgcca
     3841 ccgagtcgta ccctgaggag gaggcgctca accgcgccta cgccctgcgc gcgctggggc
     3901 gcgccgacga ggcggtcgcg gaatacgagc gggcgttcgc gctgaatccc ggaaggctcg
     3961 acgcccccaa ctcgctcgcc gtgcttgcgg ccgagcgggg ggactgggat cgggcggtgg
     4021 tccgctaccg ggaggcgctg gcgatcgatc ccggcttcgt cgatctgcgc cgcagcctcg
     4081 gcgaggcgtt gctcgcgctc ggccgccgcg aggaggcgcg ccaggaatgg accatcgccg
     4141 ccgggctggc tccggcggcc gggctcgttc tggccgacct ggcgctgctc gacctggagg
     4201 agggggcgct gcagccggcg gaggatcggg cgctcgcggc ggtgcgggcc cgtcccgacc
     4261 tgccggagac ccacatggcg ctcgggatcg tctcgcgggc gctgcggcgc cgtgaccggg
     4321 cgatggtctc gttcgagacg gcggcacgcc tcttcccggc cggatcgacc ggccgcgccc
     4381 gcgccaccga atggctggac cggatgcgcc gccgcggcgc cccgtgagac aattcgcggc
     4441 acacgaagga gagcccatga aaccgatcgc ccgcgcgacg cccctcacca ccccggcacg
     4501 gcggaggctc gcagggcggg tgctccccgc ggccgcggcc gcggcgctcg cggttctcct
     4561 cggtgccgcg ccggccggcg cgcagacgac gatcgattac gacggcaacg tcgacttcac
     4621 gaagtacaaa acctactcgt gggggcgcaa cacgtcctcc gggaatcccg ccgccgacga
     4681 gcgcatcatc gcggagatcg aagcgcacct caccgccgcc ggctggaagc aggccccgcc
     4741 cgatgaaggg gaggtggtgg tcgccgagca cgcgacggtc acgaacgacg tcagcgtcga
     4801 tacgttctac accggatggg ggaccggctg gggctggacc ggcttcggtc ctggcgcggg
     4861 ggtcgggacg acggtgtccc agaacctgcg ctcggggacg ctcgtcgtcg acatgttcga
     4921 cgccaccacc aagaaactca tctggcgcgg c
//
LOCUS       DASRIQ010000047         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2361283, whole genome shotgun sequence.
ACCESSION   DASRIQ010000047 DASRIQ010000000
VERSION     DASRIQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361283"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFD06_01215"
     CDS             complement(<1..421)
                     /locus_tag="VFD06_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02183.1"
                     /translation="MRSSEASPDGGAAIAEAPAPDPIGHDHCRNHARVPSVGRCEACL
                     APICQVCDFTWAGGRHFCPVCATAPREHLIAARKRSTAWSLGLAAWNSLGLLVLLGFG
                     VAGEPDGEMAETLLGYAMIFLCVLPGAVGFALALNARS"
     gene            complement(418..828)
                     /locus_tag="VFD06_01220"
     CDS             complement(418..828)
                     /locus_tag="VFD06_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02184.1"
                     /translation="MSQQCRDHPKTAAAAACAGCVDPFCEDCLVNVNGRTYCGSCKAM
                     TIAGMQAPTAGTIPCEEANQALKLAVFGIFILGPILEPMALVKARQARRRIVRDTRLA
                     GGTKVTIAASLAIAALVLWAITIASHLLVAVNTP"
     gene            complement(911..1909)
                     /locus_tag="VFD06_01225"
     CDS             complement(911..1909)
                     /locus_tag="VFD06_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HZN02185.1"
                     /translation="MTRAIACALLVLLAGGAAAEAHDVRPAYLELREGGDGGVLEVLF
                     KVPARPGGQRPLLELAWPEGAIASREEGGFQGDAWVERWRVERPGGFRGAALGVRGME
                     NGLSDVLVRFEGRQGAGRTARLTAASPDFTLADAPRRYEVAVTYARLGVEHILFGVDH
                     LLFVLALLLLTEGVWALVRTVTAFTLAHSLTLALATLGIARMPQAPVEATIALSIVFV
                     AAEIVRRRDRATAGIDPSVVAFAFGLLHGFGFAGALHDVGIPEGAIPTALLFFNLGVE
                     AGQLLFIAAALGLAQAVRRLPMTLPRPSWGWRVLPYAIGGVAVFWLLERLGALRTG"
     gene            complement(1906..>2198)
                     /locus_tag="VFD06_01230"
     CDS             complement(1906..>2198)
                     /locus_tag="VFD06_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZN02186.1"
                     /translation="VTHGRIAGLVADFTAFHGRPPDASERDGIIRAWVREEILFREAL
                     SLGLDREDAVIRDRLRQKMEFVAAGADATPESKEALYRDIARRYEVTIEEAP"
BASE COUNT          347 a          795 c          790 g          266 t
ORIGIN      
        1 ggctccgggc gttcagcgcc agtgcgaaac cgaccgcccc gggcaggacg caaaggaaga
       61 tcatggcgta gcccaggagc gtttccgcca tctcgccatc aggctccccg gccacgccga
      121 atccgagaag gaccagcagg ccgagcgagt tccacgccgc gaggccgagg ctccaggccg
      181 tggagcgctt gcgtgccgcg atcaggtgct cgcggggcgc cgtggcgcag accgggcaga
      241 agtgccgccc ccccgcccag gtgaaatcgc agacctggca gatcggcgcg aggcacgctt
      301 cgcagcgccc gacgctgggt acgcgcgcgt ggttccggca gtggtcgtgc ccgatcggat
      361 cgggagccgg cgcctccgcg atggcggccc ctccatcggg ggaagcctcg ctggacctca
      421 aggagtgttc acggcgacga ggaggtgcga cgcgatggtg atcgcccaga gaaccaacgc
      481 cgcgatggcg aggctcgcag cgatggtcac tttggtgccg ccggcgaggc gcgtgtcccg
      541 aacgatgcgg cgccgcgcct ggcgcgcctt gacgagcgcc atcggctcga ggatcggccc
      601 gagaatgaag atcccgaaga cggccagctt cagtgcctgg ttcgcctcct cgcacgggat
      661 cgtccccgcc gtgggggcct gcatccctgc gatggtcatg gccttgcacg aaccgcagta
      721 cgtccgcccg ttcacgttga cgaggcagtc ctcgcagaac ggatcgacac accccgcgca
      781 tgcggccgcg gcggcggtct tcgggtgatc ccggcactgc tgtgacaccg atcctcccgg
      841 cgaaggagcg attcggtcct gagcgagact cttgggcgga ttctagcccg aaatgcccgg
      901 caatcgcaga tcagccagtc cggagggccc ccaggcgctc caggagccag aagacggcga
      961 ccccgccgat ggcgtacggc aggacccgcc agccccagga ggggcgcggc agcgtcatgg
     1021 gaagccgccg cacggcctgc gcgagcccga gggcggcggc gatgaagaga agctgccctg
     1081 cttcgacgcc gaggttgaag aagaggaggg cggtcggaat cgccccctcg gggatcccga
     1141 cgtcgtgcag cgcccccgcg aacccgaagc cgtgcaggag cccgaaggcg aaggcgacga
     1201 ccgaggggtc gattcccgcc gtcgcccgat cgcgccgcct cacgatctcc gcggcgacga
     1261 agacgatgct cagggcgatg gtcgcctcga ccggggcctg cggcatccgc gcgatcccga
     1321 gcgtcgcgag ggcgagggtg aggctgtgcg ccagcgtgaa cgcggtgacc gtccgcacga
     1381 gggcccagac cccctctgtc aggagcagga gggcgaggac gaagagcagg tgatcgacgc
     1441 cgaacaggat gtgctccacc ccgagccgcg cgtaggtgac cgccacctcg taccgccgcg
     1501 gggcgtccgc gagggtgaag tcgggagagg cggccgtcag ccgggcggtc cggccggccc
     1561 cctgccggcc ttcgaagcgg acgagcacgt cgctcagccc gttctccatg ccgcgcacgc
     1621 cgagggccgc gccgcggaag ccgcccggtc gctcgacgcg ccagcgctcc acccacgcgt
     1681 caccctggaa gccgccctcc tcgcgcgagg cgatcgcccc ctcgggccac gcgagctcca
     1741 ggagtggtcg ctggccgccg gggcgcgccg ggaccttgaa gagcacctcc aggacaccgc
     1801 cgtcgccccc ctcccgcagc tcgaggtagg cgggacgcac gtcgtgcgcc tcggcggcgg
     1861 cgccgccggc caggagcacg aggagagcgc aggcgatcgc ccgcgtcacg gcgcctcctc
     1921 gatcgtgact tcgtagcgcc gggcgatgtc gcggtagagc gcctccttcg actccggggt
     1981 cgcgtccgcc cccgccgcga cgaactccat cttctgtcgc agccgatcgc ggatgacggc
     2041 gtcttcgcga tcgagcccga gggagagcgc ctcgcggaag aggatctcct cgcgcaccca
     2101 cgcccggatg atcccgtccc gctccgaggc gtccggcggc cgcccgtgaa acgcggtgaa
     2161 atcggccacc aggccggcga tgcggccgtg cgtcacga
//
LOCUS       DASRIQ010000048         6168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_17230, whole genome shotgun sequence.
ACCESSION   DASRIQ010000048 DASRIQ010000000
VERSION     DASRIQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17230"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFD06_01235"
     CDS             complement(<1..218)
                     /locus_tag="VFD06_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZN02187.1"
                     /translation="MKIATWNVNGIRARCNEVIEWAEREKPDVICLQEIKATADQVPL
                     PLCNLPGYWCYWHGNKGYSGVGLHVRKD"
     gene            complement(298..1263)
                     /locus_tag="VFD06_01240"
     CDS             complement(298..1263)
                     /locus_tag="VFD06_01240"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZN02188.1"
                     /translation="MIFVRILVGTLLGLAALWGALAWAFRQPSRGSEPYPAAGRADAS
                     RLERHVRFLSEKGAWRSVHNAPGMSRAADYISTTFAATTATVSQQPYKAGPVETRNVI
                     ARFGLEARPRVVIGAHYDVYGDLPGADDNASGVAGLLELARLLDGMNLPTAVELVAYS
                     SEEPPFFGGPQMGSAVHVAALNSSAVKVRAMISLEMIGCFTEQQPHQGILLTTIYPRH
                     GRFIALVGRWADRDLVADAKKHFRGASPLEVVSYSGPTGLGADLSDQRNYWAAGIPAF
                     MVTDTAFMRNPNYHAETDTADTLDYQRMADVVNGVLSTVVMLARH"
     gene            1367..2017
                     /locus_tag="VFD06_01245"
     CDS             1367..2017
                     /locus_tag="VFD06_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02189.1"
                     /translation="MTKNQPPSKAGTRDVAQGLWLWRAEHPGWKPKQGWEPLVASTCV
                     ESGGETFVLDPLAPSSDESEVWARLDARPPTAVVVLKPDHVRHVDRFVRRYGARAFGP
                     DLFYRDDIPETDLEPIFPGSGLPGGVMALYDGRGRYETPLWLPEQRAIVFADALTAPR
                     GDLRVWATPWHQERVLPALRALLQLPFQHVLVSHGEPVHDRAAYERALEMPPWSGE"
     gene            complement(2040..3830)
                     /locus_tag="VFD06_01250"
     CDS             complement(2040..3830)
                     /locus_tag="VFD06_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN02190.1"
                     /translation="MSTSAEPLPAALPAMVRMLRLGWRHEPRLMLLAFGLSQLAALPD
                     ALLALWLSLLGAGILRGDRRLVVAAALGLGLSATATWFLSTVSTRVQRRFRDKVTIAL
                     ESHVARLQASIATIAHQERPEFLDRLSVLRNQVFVLDHMYMSLFSTCGWILRLVVVIA
                     LLVSIHPGLALLAVFALPTVLSSTWRPGVERAIEERGAPDARLAQHLFTVATTAAAGK
                     EVRVTGIGPRLVSERRRVWERWYGPVAAGRRTSAAWHAAAWGVFGAAYAGAIVFVTSV
                     LHAPAGDLLLLLAAGGRLTAYIGATVGEIGFLRGFWLDGSRRLAWLEDYAASLTAGSD
                     ARAPERLRDGIRLDDVSFAYPGTDRLVLEHVTVHLPAGTVVAIVGENGAGKSTLVKLL
                     AKLYEPSSGRIVADGVPLDRVRADEWRGRLAGAFQDFFRFELPARHAIGLGDVPRRDD
                     DAAVRGAAGRAGAEDLLALLPAGLDTQLGPTWPGGVDVSFGQWQKLALSRGFMRESPL
                     LLVLDEPTAALDAETEHALFERYAAAARGTAEGRITVLVSHRFSTVRMADLIVMLDGA
                     RVVQVGPHDELMARGGPYAELYRIQAAAYR"
     gene            complement(3814..5631)
                     /locus_tag="VFD06_01255"
     CDS             complement(3814..5631)
                     /locus_tag="VFD06_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN02191.1"
                     /translation="MLHDFTEQPEWRWRFFAVLPRADRGLAFAWWAILLVRGLLPAVF
                     AVAMGGLVAAVARGERPAGPLAVVAAAFMLLQVLAPIHQAVSANLGSRVAAWLFDRLT
                     EACVRPPGMGHLEDPRLTADLTTAREFDLAMTGPPMHINMDFLAAELLQTVTGVACAV
                     VLFGFAWWAPLLLGGAWFLTHRLLRESAVWRDRNTPEVREAQRHAEYAYRLAVDPPAA
                     KELRLFGLADWVMERFVTRRTQLHALQYRATRLRERPLLLSLGIVLAANLCVFWALAS
                     AALAHRLDLGQVVVFAQVAVGVSAIAFGGLSWVLDGASAPVVAVARLEPAMAVEGALA
                     PSPEPPRRAEGLPARAIRFRGVAFAYPGSDRAVLDGFDLTVPAGTSLAIVGRNGAGKT
                     TLAKLLCRLYDPTSGVIEVDGVDLRTFDVDAWRGRLSAVFQDFVRFELSLRDNVAPGG
                     APDDVVRAALAEAGATGLADLDTPLARGYPRGTDLSGGQWQRVALARALCAVRMGAGL
                     VLLDEPTAQLDVRGEAEIFARILQATRRVTTILISHRFSTVRQADRIVVLEAGRVVEL
                     GTHDELMSEGGRYRTMFDMQARRFVADADEEGTAYEHLG"
     gene            complement(5713..>6168)
                     /locus_tag="VFD06_01260"
     CDS             complement(5713..>6168)
                     /locus_tag="VFD06_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN02192.1"
                     /translation="LPARHRDLGLRLQEAGDWEARFDLLDDALADCLREADDRARVVS
                     WAVRRIEETGGTLSMRALARELGYSGRHVVTLFRDQVGIPPKLLARIVRFHRLVQHVR
                     RGTGGAWPDLALGLGFYDQAHLVRDVRQFTGVTPTEVRGMVVDLAGLLA"
BASE COUNT          971 a         2197 c         2197 g          803 t
ORIGIN      
        1 aagtccttgc gcacgtgcag gcccacgccg gaatagccct tgttgccgtg ccagtagcac
       61 cagtaaccgg gcaggttgca cagcggcagc ggcacctggt cggcggtcgc cttgatctcc
      121 tgcagacaga tcacgtccgg cttctcgcgc tccgcccact cgatcacttc attgcagcgc
      181 gcgcggatgc cgttcacatt ccaggtcgcg atcttcatcg gggagagagt agcggcggag
      241 gcggcgggag tcgaacccgg gtcggacgtc ggatccgcgg cggggcgacg gccggtgtca
      301 gtgacgcgcc agcatcacca cggtgctgag cacgccgttg acgacatccg ccatgcgctg
      361 gtagtcgagc gtgtcggcgg tgtcggtctc ggcgtgatag ttcgggttcc gcatgaacgc
      421 cgtgtcggtg accatgaacg ccgggatgcc ggcggcccag tagttgcgct ggtcggagag
      481 gtcggccccc aggccggtcg ggccggaata ggagacgact tcgagcgggc tggcgccgcg
      541 gaagtgcttt ttggcgtcgg cgacgagatc ccgatcggcc cagcggccga cgagggcgat
      601 gaaccgcccg tggcgcgggt agatggtggt caacaagata ccctggtgcg gctgctgttc
      661 ggtgaagcag ccgatcatct cgagcgagat catggcccgg accttcaccg ctgagctgtt
      721 gagcgcggcc acatggacgg cgctccccat ctgtggtccg ccgaagaagg gaggctcctc
      781 gctcgagtag gcgaccagct ccaccgcggt cggcaggttc atcccgtcga gaagccgggc
      841 cagctcgaga agtccggcca cgccgctggc gttgtcgtcg gcgccgggaa ggtctccgta
      901 gacgtcgtaa tgcgccccga tcacgacgcg aggccgcgcc tcgagaccga agcgcgcgat
      961 cacgtttctg gtttcgaccg gccccgcctt gtacggttgc tgcgacacgg tcgctgtggt
     1021 cgcggcgaac gtcgtggaga tgtagtcggc ggcgcggctc atcccgggag cgttgtggac
     1081 gctccgccat gcgccctttt cgctcaggaa gcgaacgtgt cgctcgagtc gcgaggcgtc
     1141 ggcgcgcccg gcggccggat acggctcgct gccgcggctg ggctgccgga acgcccacgc
     1201 gagcgctccc cacagcgctg cgagcccgag gagcgtgccg acgaggatgc gaacgaagat
     1261 catgcgcggt cgggcagggc ggcggccccg cgccgccgaa gatcatgggt cccgcggggc
     1321 ctggacgcaa gtcccggcgt cttatactcg atccggtcgg agaggcatga cgaaaaacca
     1381 acctccgtcg aaggcgggga cccgcgacgt ggcgcagggc ctctggctgt ggcgcgccga
     1441 acatcccggc tggaagccga aacagggatg ggagccgctc gtggcctcca catgcgtcga
     1501 gtcggggggc gagacgttcg tgctcgatcc gcttgctccg tcatccgacg agtccgaggt
     1561 gtgggcgcgc ctggacgcgc ggccgccgac cgcggtcgtc gtcctcaagc cggatcacgt
     1621 ccggcacgtc gatcgattcg tgcgccgcta cggggcgcgc gcgtttggtc ccgacctctt
     1681 ctatcgcgac gatattccgg aaaccgacct ggagccgatc ttccccggca gcggacttcc
     1741 cggtggggtg atggctctct acgacggacg gggccgttac gagacgccac tgtggctccc
     1801 ggagcagcgg gccatcgttt tcgccgatgc cctgacggcg ccccgaggcg acctgcgcgt
     1861 ctgggcgacg ccctggcacc aggagcgcgt cctgcccgcg ctcagggcac tgcttcagct
     1921 tccgttccag cacgtgctcg tctcgcacgg cgagccggtc cacgaccgcg ccgcctacga
     1981 gcgggccctc gagatgcccc cctggagcgg agagtgaggc tggaccgcct cggccatttt
     2041 cagcgatacg ccgcggcctg gatgcggtag agctcggcat aggggccgcc ccgcgccatc
     2101 agctcgtcgt gcggcccgac ctggacgacc cgggcgccgt cgagcatcac gatgaggtcg
     2161 gccatccgca ccgtgctgaa gcggtgcgac acgaggaccg tgatgcgtcc ttccgccgtg
     2221 ccgcgggccg ccgcagcata gcgctcgaac agggcgtgct cggtctcggc gtcgagcgcc
     2281 gcggtcggct cgtcgagcac cagcagcagc ggagactcgc gcatgaagcc gcgcgacagc
     2341 gcgagtttct gccattgccc gaacgaaacg tccacgcccc ccggccaggt ggggccgagc
     2401 tgggtgtcga gtcccgcggg caggagggcg aggaggtctt ccgccccggc gcgtccggcg
     2461 gcaccgcgga cggccgcatc gtcgtcgcga cgcggcacat cgccgagccc gatcgcatgg
     2521 cgtgcgggca gctcgaagcg gaagaaatcc tggaacgccc ccgcgagccg cccgcgccac
     2581 tcgtccgccc ggacccggtc gagcggcacg ccgtcggcga cgatgcggcc cgaggaaggc
     2641 tcgtacagct tcgcgagcag cttgacgagc gtcgacttgc cggcgccgtt ctcgccgacg
     2701 atcgcgacca ccgtaccggc cggcaggtgg acggtgacgt gctcgagcac cagtcggtcg
     2761 gtgcccgggt aggcgaacga gacgtcgtcg agccggatgc cgtcgcgcag ccgttccggg
     2821 gcgcgcgcgt cggacccggc ggtcagcgag gcggcgtagt cctcgagcca ggcgagacgg
     2881 cgcgatccat cgagccagaa tccgcgcagg aagccgatct cccccaccgt cgccccgatg
     2941 taggcggtga ggcgcccgcc ggcggcgagc agcagcagga gatcccccgc cggcgcgtgc
     3001 agcaccgagg taacgaagac gatcgctccg gcataggccg cgccgaatac tccccaggcg
     3061 gccgcgtgcc aggcggcgct cgtccgtcgc ccggcggcga ccgggccgta ccagcgctcc
     3121 cagacgcggc gccgctcact cacgagacgc gggccgatcc cggtgacgcg gacctccttg
     3181 ccggcggcgg cggtcgtggc gacggtgaag agatgctggg ccaggcgcgc gtcgggcgcg
     3241 ccgcgctcct cgatcgcccg ctcgaccccc gggcgccagg tggaggagag gaccgtgggc
     3301 agtgcgaaga ccgccagcag ggcgaggccc ggatggatcg acacgagcag ggcgatcacg
     3361 accacgagcc gcaggatcca gccgcatgtg gagaagagcg acatgtacat gtggtcgagg
     3421 acgaacacct ggttgcgcag caccgaaagg cgatcgagga actcgggacg ctcctggtgg
     3481 gcgatggtgg cgatcgacgc ctgcaaccgc gccacgtgcg actcgagcgc gatcgtcacc
     3541 ttgtcgcgga agcgccgctg cacgcgcgtg ctgacggtgc tcaggaacca cgtggcggtc
     3601 gcggacaggc cgaggccgag tgccgcagcg acgacgaggc gccggtctcc ccggaggatc
     3661 cccgcaccca ggagactcag ccacagcgcc aggagcgcgt ccggcagcgc cgcgagctgc
     3721 gacagcccga aggcgaggag catcagccgc ggctcgtgcc gccagccgag gcgaagcatc
     3781 cgcaccatcg cggggagcgc cgccgggagc ggctcagccg aggtgctcat aggccgtccc
     3841 ttcctcgtcc gcgtcggcga cgaaacgccg cgcctgcatg tcgaacatcg tccggtagcg
     3901 tcccccctcg gacatcagct cgtcgtgcgt tcccagctcg acgactcgtc ccgcttcgag
     3961 cacgacgatg cggtcggcct ggcgcaccgt cgagaagcgg tgcgagatca ggatggtcgt
     4021 gacccggcgc gtcgcctgaa ggatgcgcgc gaagatctcc gcctcgccgc gcacgtcgag
     4081 ctgcgccgtc ggctcgtcga gaagcaccag ccctgccccc atccggaccg cgcacagcgc
     4141 ccgggccagc gccacccgct gccactgccc tcccgacaga tccgtgccgc gggggtagcc
     4201 gcgagcgagc ggggtgtcga gatcggccag gccggtcgcc cccgcctccg cgagcgccgc
     4261 gcgcacgacg tcgtccgggg ccccgcccgg cgcgacgttg tcgcgcagcg acaactcgaa
     4321 gcgcacgaaa tcctggaaga ccgccgacag tcgtccgcgc caggcgtcga cgtcgaatgt
     4381 gcgcaggtca acgccgtcca cctcgatgac gccggaggtc gggtcgtaga ggcggcagag
     4441 gagcttcgcc agcgtcgtct tgccggcgcc gttcctcccg acgatcgcga gcgacgtgcc
     4501 ggccggcacc gtcaggtcga agccgtcgag gacggcccga tcgcttcccg gatacgcgaa
     4561 ggcgacgccg cggaaccgga tggcgcgcgc cggcagtccc tccgcacgcc ggggcggttc
     4621 cggggagggc gcgagcgccc cttcgacggc catcgccggc tcgaggcgcg ccaccgccac
     4681 gacaggggcg gaggcgccgt cgagcaccca gctcaacccg ccgaacgcga tggcgctcac
     4741 cccgaccgcc acctgggcga agaccacgac ctgccccaga tcgagcctgt gggcgagcgc
     4801 cgccgaagcg agcgcccaga agacgcagag attcgcggcc aggacgatgc cgaggctgag
     4861 caggagcggt cgctcccgca ggcgcgtcgc ccggtactgc agagcgtgca gctgcgtccg
     4921 gcgcgtcacg aagcgctcca tcacccagtc ggcgagcccg aagaggcgca gctccttcgc
     4981 cgccggcgga tcgacggcca ggcggtaggc gtactccgcg tgccgctgcg cctcgcgcac
     5041 ttccggcgtg ttgcgatccc gccagacggc gctctcccgg agcagccggt gcgtcaggaa
     5101 ccacgccccc ccgagcagca gcggggccca ccaggcgaag ccgaagagga ccacagcgca
     5161 cgcgaccccg gtgaccgtct gcagcagctc tgccgccagg aagtccatgt tgatgtgcat
     5221 cggcggaccg gtcatcgcga gatcgaactc gcgcgccgtc gtcaggtccg cggtgagccg
     5281 cggatcctcc aggtgcccca tcccgggggg cctcacgcac gcctcggtca ggcggtcgaa
     5341 gagccacgcc gccacgcgac tgccgagatt ggcgctcacc gcctggtgaa tcggcgccag
     5401 cacctggagc agcatgaacg ccgccgccac gaccgcgagc ggtccggccg gccgctcgcc
     5461 gcgcgcgacc gccgcgacca gcccccccat ggcgaccgcg aagacggcgg ggagcaggcc
     5521 gcgcacgagc aggatcgccc accaggcgaa ggccaggcct cgatccgcgc ggggcaggac
     5581 ggcgaagaac ctccacctcc actccggttg ctcggtgaaa tcgtgcagca tcccttcctc
     5641 cggtatcgat cgcggatgtt ctatcaggat agccgcgccg cgtcttggaa aaatcggaac
     5701 tgccgaatcg ggctaggcca ggaggccggc gaggtcgacg accatccccc gcacctccgt
     5761 cggcgtgacg ccggtgaatt ggcgcacgtc acgcacgaga tgggcctggt cgtagaagcc
     5821 gagaccgagg gcgagatcgg gccaggcgcc gcctgtccca cggcgcacgt gctgcaccag
     5881 gcgatggaag cggacgatgc gggccaggag cttcggcggg atcccgacct ggtcgcggaa
     5941 cagcgtgacg acgtgccggc cgctgtaacc cagctcgcgc gccagcgcgc gcatgctcag
     6001 cgttccgccg gtctcctcga tgcggcgcac ggcccaggag acgacccgtg cgcggtcgtc
     6061 cgcttcgcgc agacaatcgg cgagcgcatc gtccaggaga tcgaaacggg cctcccagtc
     6121 gcccgcctcc tgcaaccgga gacccaggtc gcgatgccgt gccggcaa
//
LOCUS       DASRIQ010000049         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1838006, whole genome shotgun sequence.
ACCESSION   DASRIQ010000049 DASRIQ010000000
VERSION     DASRIQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838006"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VFD06_01265"
     CDS             <1..1268
                     /locus_tag="VFD06_01265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZN02193.1"
                     /translation="AVALKGEPVLPYVWHYLRFLPTLEVYAIPLALGLALLYAASGAA
                     GFAPLGGTILVAAALLKKLNRARSELSRALEQVQSRTRELKTVNTIGREISASLDPQV
                     VFSKIAFQLTRILDAPYLILSLRQRGAIETYVEFVARDGRVQPRPERPLGEGFTHWIV
                     EAKRPLMLTDLQEERDSLPCAPVVLAPEVRSILAAPLLTEHGQDAIGVLCVQSPRPGA
                     YGVEHVSVLTTVAQQAAVAIDNARNYQLATTDQLTHLYLRDFFRRKLQEEQARAQRYG
                     SGFAVVMVDLDRFKQINDRLGHLAGDRYLQKVGGVVCDTMRAADIPCRWGGEEFAVLL
                     PETDLEGARSIAERLQARIGSLRMMIGDAVVQTTVSAGIACYPQDTVDGSLDVLLERA
                     DKALYAAKQAGRDRIVAAEHLPPRPEKAA"
     gene            complement(1296..1610)
                     /locus_tag="VFD06_01270"
     CDS             complement(1296..1610)
                     /locus_tag="VFD06_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02194.1"
                     /translation="MVLPFLKPPAVVIVYLQAPRERYWGVVRALDATGVVLQGTDLDS
                     FDDWMRLVASGEPQHEPSTVFFPMHRVEKLLVDAPAGAAPSLQQQFESRLGRTLLSYL
                     GS"
     gene            complement(1616..2284)
                     /locus_tag="VFD06_01275"
     CDS             complement(1616..2284)
                     /locus_tag="VFD06_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HZN02195.1"
                     /translation="MKSGSSSERLVTRAAAELKDGDYVNLGIGMPTLVANHVPAGMHV
                     TFQSENGMLGVGPFPLDAEVDPDLINAGKQTVSETPGCSYFSSADSFAMIRGGHVDLS
                     ILGGMEVDEEGNLANWMVPGKMVKGMGGAMDLVAGAKRVVVMMTHTTKEGAPKILKVC
                     RLPLTGVKVVHRIITEMAVIDVTPEGLVLREVAPGVTEAEVAAATEARLVPDPAGVKP
                     MQGA"
     gene            complement(2235..2939)
                     /locus_tag="VFD06_01280"
     CDS             complement(2235..2939)
                     /locus_tag="VFD06_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZN02196.1"
                     /translation="MNKVVRSADEAIADLRDGASIMMGGFGVCGIPEHLIAALVRRGT
                     RDLTVMSNNAGVSDFGIGLLLQSRQVRKMIATYVGENELFERQFLSGELEVELVPQGT
                     FAERIRAGGAGIPAFFTPTGYGTKAAEGKETREFDGRHFVMERALHADYAFVKAWKGD
                     RLGNLVYRRTTRNFNPMMATAGRITIAEVEQLVEPGALDPDQVHTPGIFVQRLFQGSA
                     YEKRIEQRTTRDAGRR"
     gene            complement(2936..>4188)
                     /gene="meaB"
                     /locus_tag="VFD06_01285"
     CDS             complement(2936..>4188)
                     /gene="meaB"
                     /locus_tag="VFD06_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZN02197.1"
                     /translation="LRRLYGRTGRASIVGVTGPPGAGKSTLVNRLIAHHRSAGRRVGV
                     VAVDPSSAFSGGAILGDRIRMMEHAVDDGVFIRSMASRGQFGGLSRATRDAADLLDAS
                     GFDPVMIETVGVGQDEIDVVRVADTVLVVLTPGQGDEVQAIKAGLLEIADVFVINKAD
                     HAGSDRLASDLEGMLALGEARPWKPPIVRSVATDGTGIADIVAAVERQAAWLDASGAR
                     GARRREGLRLRLHDILRGRVLDRLASDAPARRSLEEWEEKVARRDVDPYTAAAALLDQ
                     GRGRSALDHVGVAVSRLDERLPLYRDLLGLPHLETEEVPTEGVRAAFLPAGRTRIELL
                     EPTRDDAALKRHLERRGEGVHHLCFEVDDLDGALERFRAAGHEIVGGGARPGAEGTRV
                     AFVHPKRTGGVLIELRATRKEGRS"
BASE COUNT          609 a         1552 c         1400 g          627 t
ORIGIN      
        1 tggccgtcgc gctcaagggg gagccggtgc tgccgtacgt ctggcactac ctgcgctttc
       61 tgccgacgct cgaggtgtac gcgatcccgc tggcgctcgg cctggcgctg ctgtatgcgg
      121 cctcgggagc ggccggcttc gccccgctcg gcggcaccat cctcgtcgcc gcggcgctcc
      181 tcaagaagct caaccgggcc cgctccgagc tgagccgcgc cctcgagcag gtgcagtcgc
      241 gcacccgcga gctgaagacg gtcaacacca tcggccggga gatcagcgcc agcctcgatc
      301 cccaggtcgt cttctccaag atcgccttcc agctgacgcg catcctcgac gcgccatacc
      361 tcatcctgtc gctgaggcag cgcggagcga tcgagaccta cgtcgagttc gtggcgcggg
      421 acggccgcgt gcagccccgt ccggagcggc cgctcgggga agggttcacc cactggatcg
      481 tcgaggcgaa gcggcccctg atgctgaccg atctccagga ggagcgggac tcgctcccct
      541 gcgccccggt cgtgctcgct ccggaggtgc gatccatcct cgccgccccc ctgctgaccg
      601 agcacgggca ggacgcgatc ggcgtgctgt gcgtgcagag cccgcgcccc ggcgcctacg
      661 gcgtcgagca cgtgtcggtc ctgacgacgg tggcgcagca ggccgccgtc gcgatcgaca
      721 acgcccgcaa ctaccagctg gcgaccaccg atcagctcac gcacctgtac ctgcgcgact
      781 tcttccgccg caagctgcag gaggagcagg cgcgcgccca gcgctatggc agcggcttcg
      841 ccgtggtcat ggtggacctc gaccggttca agcagatcaa cgatcggctc ggccatctgg
      901 ccggggaccg atacctgcaa aaagtcggcg gcgtggtgtg cgacaccatg cgagcggcgg
      961 acatcccctg ccgctggggc ggcgaagagt tcgccgtcct ccttccggaa accgacctcg
     1021 agggggcgcg ttccatcgcc gagcgcctgc aggcccgcat cgggtcgctg cgcatgatga
     1081 tcggcgacgc ggtggtgcag acgacggtca gcgccggcat cgcctgctac ccccaggaca
     1141 ccgtcgacgg atcgctcgac gtgctcctgg aacgggccga caaggcgctc tacgccgcca
     1201 agcaggccgg tcgggatcgc atcgtcgcgg ccgagcacct gccgccgcgc cccgagaagg
     1261 cggcctagcg acccgcgagg ggtgcagacc gccgatcagg atcccaggta ggagagaagg
     1321 gtccgcccca ggcgcgactc gaactgttgc tgcagcgacg gggcggcgcc ggccggcgcg
     1381 tccaccagca gcttctccac ccggtgcatc ggaaagaaga cggtgctcgg ctcgtgctgc
     1441 ggctcgccgg aggcgaccag ccgcatccag tcgtcgaagg agtcgaggtc ggtcccctgc
     1501 agcaccactc ccgtggcgtc gagggcccgc accactcccc agtagcgctc gcgcggggcc
     1561 tggaggtaga cgatgacgac cgccgggggt ttcaggaacg gcaacaccat cgccgtcagg
     1621 ccccctgcat gggcttgact cccgccgggt ccggaacgag gcgcgcctcg gtcgcggcgg
     1681 cgacctcggc ctcggtgacg cccggcgcca cctcgcgcag caccagcccc tccggcgtga
     1741 cgtcgatgac cgccatctcg gtgatgatgc ggtgcacgac cttcacgccg gtcagcggca
     1801 ggcggcagac cttgagaatt tttggggccc cctccttggt cgtgtgggtc atcatgacca
     1861 cgacccgctt cgcgccggcg acgaggtcca tcgccccgcc catccccttg accatcttcc
     1921 ccggcaccat ccagttcgcc aggttcccct cctcgtccac ctccatcccg ccgaggatgc
     1981 tgagatcgac gtggccgccg cggatcatgg cgaacgagtc ggcgctgctg aaataggagc
     2041 agcccggcgt ctcgctcacg gtctgcttgc cggcgttgat gagatcggga tcgacctccg
     2101 cgtcgagcgg gaacggaccg acgccgagca tgccgttctc cgactggaag gtcacgtgca
     2161 tccccgccgg cacgtggttg gcgacgaggg tcggcatgcc gatccccagg ttgacgtagt
     2221 cgccgtcctt cagctcagcg gcggcccgcg tcacgagtcg ttcgctgctc gatccgcttt
     2281 tcataggcgc tcccctggaa gaggcgctgc acgaagatcc ccggcgtgtg gacctgatcc
     2341 gggtcgagcg cccccggttc gacgagctgc tcgacctcgg cgatcgtgat ccgtcccgcc
     2401 gtcgccatca tcggattgaa attgcgcgtc gtccggcggt agacgaggtt cccgagccga
     2461 tcccccttcc acgccttcac gaaggcgtag tcggcgtgaa gcgcccgctc catgacgaag
     2521 tggcggccgt cgaactcgcg cgtctccttc ccctccgccg ccttcgtgcc atagccggtc
     2581 ggggtgaaga aggccgggat gccggcgccg ccggcgcgga tccgctcggc gaaggtcccc
     2641 tgcggcacca gctccacttc gagctcgccg gagaggaact gccgctcgaa cagctcgttc
     2701 tcgccgacgt aggtggcgat catcttccgc acctggcgac tctggaggag caaaccgatg
     2761 ccgaagtcgc tcacaccggc gttgttgctc atcaccgtga ggtcgcgcgt gccacggcgc
     2821 accagcgccg cgatcaggtg ctccgggatg ccgcagacgc cgaatccccc catcatgatg
     2881 gaagcgccgt cccggagatc ggcgatcgcc tcgtccgcgc tccgcacgac tttgttcatg
     2941 accgcccttc ttttcgggtg gcgcgcagct cgatgaggac gccgccggtg cgtttcgggt
     3001 gcacgaacgc cacccgcgtc ccctcggccc ccggccgcgc cccacccccg acgatctcgt
     3061 gtcccgccgc ccggaagcgc tcgagggcgc cgtccaggtc gtccacctcg aagcagagat
     3121 ggtgcacccc ttcgccgcgc cgctcgaggt ggcgtttcag cgcggcgtcg tcgcgcgtgg
     3181 gctccaggag ttcgatgcgg gtgcggccgg ccggcaggaa ggcggcccgc accccttccg
     3241 tcgggacctc ttcggtctcg agatgcggca gaccgaggag atcccggtac agcggcaggc
     3301 gctcgtcgag gcggctcacc gccacgccca cgtgatcgag cgcggaccgt ccgcgtccct
     3361 gatcgagcag agccgcggcg gccgtatagg gatcgacgtc ccgccgcgcc accttctcct
     3421 cccactcctc gagcgatcgg cgcgccggag cgtcggaggc gagccgatcg agaacccggc
     3481 cgcgcaggat gtcgtgcagg cgcaggcgca gcccttcgcg ccgccgcgca ccgcgtgccc
     3541 cgctcgcatc gagccaggcg gcttgccgtt cgacggcggc gacgatgtcg gcgatcccgg
     3601 tgccgtcggt cgcgacgctc cggacgatcg gcggcttcca cggccgcgcc tcgcccagcg
     3661 ccagcatccc ctccagatcg gaggccagcc tgtcgctccc ggcgtggtcc gccttgttga
     3721 tgacgaagac gtcggcgatc tccaggagcc ccgccttgat cgcctgcacc tcgtccccct
     3781 gccccggcgt caggacgacg agcacggtgt cggcgacccg cacgacgtcg atctcgtcct
     3841 gaccgacgcc gaccgtctcg atcatcaccg ggtcgaatcc ggacgcatcg agcaggtccg
     3901 cggcgtcgcg cgtggcgcgc gacaggccgc cgaactgccc gcggctcgcc atcgaccgga
     3961 tgaagacccc gtcgtcgacg gcgtgctcca tcatccggat gcggtcgccg aggatggctc
     4021 cgccggagaa cgcgctcgag ggatcgaccg ccaccacccc caccctgcgg ccggcgctac
     4081 ggtgatgggc gatgaggcgg ttgacgaggg tgctcttgcc ggccccggga ggaccggtga
     4141 cgcccacgat ggaagcccgg ccggtgcgtc cgtagaggcg gcgcagca
//
LOCUS       DASRIQ010000050         5027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1328324, whole genome shotgun sequence.
ACCESSION   DASRIQ010000050 DASRIQ010000000
VERSION     DASRIQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328324"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..827
                     /locus_tag="VFD06_01290"
     CDS             48..827
                     /locus_tag="VFD06_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HZN02198.1"
                     /translation="MVVERGLAPSRERAQALILAGRVIVDGRPAGKPGGTVPGDAAIA
                     LLAPDHPFVGRGGLKLEGALTRLGVDPHGLVALDVGASTGGFTDCLLKRGARRVYALD
                     VGTGQLDWSLRRDPRVVTMEGRNARHLRPEDLPEPVDLAVVDVSFISLALVLPPLRPL
                     VKTGGTVLALVKPQFEVGRRDVGKGGIVRDPALHRGAVASVARSAAAAGYALLGGCRS
                     PLPGAEGNVEFFLLLGPGPRAGAAGDAEALARSIVDEERGA"
     gene            827..1687
                     /locus_tag="VFD06_01295"
     CDS             827..1687
                     /locus_tag="VFD06_01295"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZN02199.1"
                     /translation="MPRQTRGPIERVLLVGKRDSPRATAEVRRLARFLEGLDVGVVYD
                     AGSARAVGARPARDLEGVGLCVVVGGDGTLLAAARSLAGRPIPILGVNLGGLGFMAET
                     GPRQAVARLRQVLAGRHEVGRRMTLEATLVRGRRPGLKQSALNDVVIAKSAIARMIEL
                     KVKINGSLVTTYRADGLIVSTPTGSTAYSLSAGGPIILPDMDALLISPICPHTLSMRP
                     LVVPGDARVELTLASEGSKVYLTLDGQVGQMLEPGDRVRVRRGPADVLMVRPRRLNYF
                     DVLRRKLRWG"
     gene            complement(1712..2224)
                     /locus_tag="VFD06_01300"
     CDS             complement(1712..2224)
                     /locus_tag="VFD06_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02200.1"
                     /translation="MSEAGETGGPQADSGVRAPRTVPVAALAGAVVLIGLVGFALYRR
                     GTTSAPGAAPAPATADSGAAPTDEGGTPIVAQTPFRLSPEASILAERYRCVCGCNDNL
                     SVCTCRNPNAAEDMKRLLQGLANEGKTPEEADAAMEAKFGAASLLKNPAPPQTLPSHA
                     GQAPAPASKH"
     gene            2299..3606
                     /locus_tag="VFD06_01305"
     CDS             2299..3606
                     /locus_tag="VFD06_01305"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN02201.1"
                     /translation="MSLRAPRREVAVWCLYDFADSAFTTLIVTVTYALYFRGVVAADK
                     GAAASTYWGLSISLSMALVALLAPLFGAVADFAGRKKLFLGLCAGTAIVFTALLRLVG
                     PGDIALGMALFILANVGYEGAHVFYNGFLLEIASDRELGRVSGYGWAVGYLGGLLALG
                     LSLPLTRDGFGPAGIDRYRWTFPLVALFHLTFALPLFLWLKERAPRRPVRFAAMVREG
                     TTRLATTLRSVRRYPDLLRFLLAFFIYNDGVMTVISFGAIYALNVIGFTVLQVTLLFF
                     VMQLTAAVGAFVAGRLVDSLGPRKTILMTLGLWCFVVLGAFLSRSVPQFFAVCVGASI
                     GMGSTQTASRSLMALLVPPGRSAEFFGFYGLTGKVSAIFGPLAFGAVAAFAGSERPAV
                     LSVLPFFAIGYVLLLRVDVDRARAASGRLDAPTEVSPVAGVAS"
     gene            3646..4083
                     /locus_tag="VFD06_01310"
     CDS             3646..4083
                     /locus_tag="VFD06_01310"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZN02202.1"
                     /translation="MIDRLQNDLKDSMRRGDALATSTLRLLLSQMQYAKIQAGRPLTE
                     AETIAVLERAVKTRREAIEQYERGGRPELAEKERAEIGVVERYLPPPLTPEETTAAVE
                     ALITELGAKDKKDLGRVMKEFQTRHRGRADGKTVSALIASRLS"
     gene            4094..>5027
                     /locus_tag="VFD06_01315"
     CDS             4094..>5027
                     /locus_tag="VFD06_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02203.1"
                     /translation="MAARPSAPPRTGAWRFPAAALGLLVVALTLAPIGNNDLFLHLVT
                     GQVVLERHEVPRTDDYSALARGRPYVAHEWLAAVLFRLVQRAGSGEGFDRLVLAKVAL
                     SLALAWLLLRAARHAGASRPVALAGLAWVLCLAAARLQERPHLFAYLLLAAFVLVLAR
                     RRRRLAAGRSDRGLFLLPLLQVLWANLHGSFLLGPAIVLLAAAGSAFERLLPGRSSAA
                     RDEGATRAETLRLAALALVLVPLCLVNPYGVDLLRFPFALTGSRFMKQIYEWLPPYDE
                     AFRGTYMARLYVIWAAVSAAVWIASLARPRRLPRG"
BASE COUNT          572 a         1843 c         1813 g          799 t
ORIGIN      
        1 accccgcatc gtcccctccc cgcccggcga agacgcggct cgatcgactg gtggtcgagc
       61 gcggcctggc gccgagccgc gagcgcgccc aggccctgat tctggcgggc cgcgtcatcg
      121 tcgacgggcg gccggcgggg aagccgggag ggacggtgcc gggagacgcg gccatcgcac
      181 tcctcgcccc ggatcatccg ttcgtgggac gcggcggcct caagctcgag ggggcgctca
      241 cccgcctcgg cgtcgatccc cacgggctcg tcgccctcga cgtgggagcc tccacaggcg
      301 ggttcaccga ctgccttctg aagcgcggcg cgcgccgcgt ctacgccctc gacgtcggga
      361 ccggacagct cgactggtcg ctgagacgcg atcctcgcgt cgtgacgatg gaggggagga
      421 acgcgaggca cctccggccg gaggacctgc cggagccggt cgacctggcg gtcgtggacg
      481 tgagcttcat ctcgctggcg ctggtgctgc cgccgctccg accactcgtc aagaccggcg
      541 gcacggtcct cgcgctggtc aagccgcagt tcgaggtcgg gcggcgtgac gtcggcaaag
      601 gggggatcgt ccgggacccg gcgctgcacc gcggggcggt ggcgtcggtg gcgcggtcgg
      661 ccgccgcggc cggctacgcc cttctcggcg gctgccggtc ccctcttccg ggagcggagg
      721 ggaatgtgga attcttcctc ctcctcggcc ccggtccgcg cgccggagcg gcgggggacg
      781 ccgaggcgct ggcacggtcg atcgtcgacg aggagcgggg ggcgtgatgc cgagacagac
      841 ccgggggccg atcgaacgcg tcctgctcgt cggcaagcgg gacagcccgc gcgcgacggc
      901 ggaggtgcgc cgcctcgccc gcttcctcga agggctcgac gtcggcgtgg tgtacgacgc
      961 cggcagcgcc agggcggtcg gcgcgcgccc ggcgcgcgac ctcgagggcg tggggctctg
     1021 cgtcgtcgtc ggcggcgacg ggacgctcct cgcggccgcc cgcagcctcg cggggcgccc
     1081 gatcccgatc ctgggggtca acctcggagg gctcggcttc atggcggaga ccggcccgcg
     1141 ccaggcggtg gcccgccttc ggcaggtgct ggcgggacgc cacgaggtcg ggcggcgcat
     1201 gaccctcgaa gcgaccctcg tgcgcggccg gaggcccggc ctgaagcagt cggcgctgaa
     1261 cgacgtggtg atcgccaaga gcgcgatcgc ccgcatgatc gaactgaaag tcaagatcaa
     1321 cggcagcctc gtcaccacct accgtgcgga cgggctcatc gtttccactc cgaccggatc
     1381 gaccgcctat tccctctcgg ccggcgggcc gatcatcctc ccggacatgg atgccctgct
     1441 catcagcccg atctgcccgc acacgctgag catgcggccg ctggtggtcc cgggcgacgc
     1501 ccgggtcgag ctgaccctgg cgagcgaagg ctcgaaggtc tacctgacgc tcgacggaca
     1561 ggtcggccag atgctcgagc cgggggaccg ggtgcgggtg cgccgcgggc cggcggacgt
     1621 cctcatggtc cggccgcggc gcctgaacta cttcgacgtc ctgcgccgca agctccgctg
     1681 gggctgagga cgctcggtcg ggtcggagag atcagtgctt cgaggccggt gcgggcgcct
     1741 gaccggcgtg cgacggcagg gtctgcggcg gcgccgggtt cttcaggaga gacgcggcgc
     1801 cgaacttggc ctccatcgcg gcatccgcct cttcgggggt cttcccctcg ttcgccagcc
     1861 cctgcaggag ccgcttcatg tcctcggcgg cattggggtt gcggcaggtg cagaccgaga
     1921 ggttgtcgtt gcagccgcag acgcagcggt agcgctccgc caggatgctc gcttccgggc
     1981 tcagccggaa cggcgtctgg gcgacgatcg gggttcctcc ctcgtcggtc ggggccgcgc
     2041 cgctgtcggc agtcgcgggc gcgggtgcgg cgccgggagc gctcgtggtg ccgcgacggt
     2101 acagtgcgaa cccgacgagg ccgatcagga cgaccgcccc cgccagcgcc gccaccggca
     2161 cggtgcgggg cgccctgacc ccggagtcgg cctgcggtcc gcccgtttcg cccgcctcgc
     2221 tcatcccctc accagcgtcg ccaaagctcg cggatgatag cacgccgccc tcgagcctct
     2281 cctctgtatg atggcgcggt gagcctgcgc gccccccgtc gcgaggtcgc agtctggtgc
     2341 ctttacgact tcgccgactc cgcgttcacc acgctcatcg tcacggtcac ctatgcgctc
     2401 tacttccggg gcgtcgtcgc ggccgacaag ggggcggcgg cgagcacgta ctggggcctg
     2461 tcgatctccc tgtcgatggc actcgtcgcc ctgctcgccc cgctcttcgg cgcggtggcc
     2521 gacttcgccg ggcgcaagaa gctgttcctc ggactgtgcg ccggcaccgc catcgtgttc
     2581 acggcgctcc tgcgcctcgt cgggccgggg gacatcgcgc tgggcatggc gctgttcatc
     2641 ctcgcgaacg tcggctacga gggggcgcac gtcttctaca acgggttcct gctcgagatc
     2701 gcctcggacc gggagctggg gcgggtctcg gggtacggct gggcggtcgg ctacctcggg
     2761 ggccttctgg cgctcggtct cagcctgccg ctgacgcgcg acggcttcgg gcccgcgggg
     2821 atcgatcgtt accgctggac gtttccgctc gtcgccctgt tccacctgac gttcgcgctg
     2881 ccgctcttcc tctggctgaa ggagcgggcg ccgcggcggc ctgtgcggtt cgccgcgatg
     2941 gtccgcgagg ggacgacccg cctcgccacg acgctgcggt cggtgcggcg ctacccggat
     3001 ctcctgaggt tcctgctcgc cttcttcatc tacaacgacg gggtgatgac ggtcatctcc
     3061 ttcggcgcga tctacgcgct gaacgtcatc ggcttcaccg tcctccaggt gacgcttctg
     3121 ttcttcgtga tgcagctcac cgccgccgtc ggcgccttcg tcgccggccg gctggtggac
     3181 agcctcgggc cgcgcaagac catcctcatg acgctcgggc tgtggtgctt cgtcgtgctg
     3241 ggcgccttcc tgtcgcgctc ggtgccgcag ttcttcgccg tctgcgtcgg cgcctcgatc
     3301 ggcatggggt cgacgcagac cgccagtcgc agcctcatgg cgctcctcgt cccacccggc
     3361 cggagcgccg aattcttcgg cttctacggc ctgaccggca aggtctcggc gatcttcggg
     3421 ccgctggcct tcggcgccgt cgccgccttc gccggcagcg agcgtcccgc cgtcctttcg
     3481 gtgctgccgt ttttcgcgat cgggtacgtg cttctgctgc gggtcgatgt cgatcgggcg
     3541 cgcgctgcgt cggggcggct ggacgccccg acggaggtct cccccgtcgc cggtgtggca
     3601 tcataggggc cgccgcgccg tgagcggcgc ccccggagcc acgccgtgat cgatcgactg
     3661 cagaacgacc tcaaggattc gatgcgccgc ggcgacgcgc tcgccacctc caccctgcgc
     3721 ctcctgctct cgcagatgca gtacgcgaag atccaggcgg ggcgcccgct caccgaggcg
     3781 gagacgatcg cggttctcga gcgggcggtg aagacgcggc gcgaggcgat cgagcagtac
     3841 gagcgcggcg ggaggcccga gctggcggag aaggagcgtg ccgagatcgg cgtcgtcgag
     3901 cgctacctgc caccgccgct gacgcccgag gagaccaccg ccgccgtcga ggcgctcatc
     3961 accgagctcg gcgcgaagga caagaaggac ctcggccgcg tcatgaagga gttccagacg
     4021 cgccaccgcg gacgcgccga cggaaagacg gtcagcgccc tcatcgcgtc gcgcctctcc
     4081 taggctcccg ggagtggccg cgcgtccttc ggcgcctccc cggactggcg cgtggcgctt
     4141 cccagcggcg gctctcggcc tcctcgtcgt cgccctgacc ctcgcgccca tcggcaacaa
     4201 cgacctcttc ctgcacctcg tcacgggaca ggtcgtcctc gagcgtcacg aggtcccgcg
     4261 gaccgacgac tactccgccc tggcgcgcgg ccggccgtac gtggcgcacg agtggctggc
     4321 cgccgtgctg ttccggctcg tgcagcgggc cgggagcggc gaggggttcg accgcctggt
     4381 gcttgcgaag gtggcgctgt cgctcgcgct ggcctggctg ctgctgcgcg ccgcgcggca
     4441 cgcgggcgcc tcgaggccgg tcgcgctcgc cgggctggcg tgggtcctgt gcctggccgc
     4501 cgcgcggctt caggagagac cgcacctgtt cgcctacctc ctgctcgcgg ccttcgtcct
     4561 ggtcctcgcg cgccgccggc gtcggctcgc tgcgggacgg tcggatcgcg gcctgttcct
     4621 cctgccgctc ctgcaggtcc tctgggccaa cctgcacgga tcgttcctcc tgggcccggc
     4681 gatcgtgctg ctggccgccg ccgggagcgc cttcgagcgg ctcctccccg gccggagcag
     4741 cgcggcgcgg gacgagggcg cgacccgggc ggagacgctg cgcctcgcgg cgctcgcgct
     4801 cgtcctggtc cccctctgcc tcgtcaatcc gtacggcgtg gacctcctgc gctttccgtt
     4861 cgcgctgacc ggcagccgct tcatgaagca gatctacgaa tggctcccgc cgtacgacga
     4921 ggcgttccgc ggaacctaca tggcgcgcct ctacgtcatc tgggccgcgg tctccgccgc
     4981 ggtctggatc gcgtccctgg cgcggccgcg ccggctcccg cgcggcg
//
LOCUS       DASRIQ010000051         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_275636, whole genome shotgun sequence.
ACCESSION   DASRIQ010000051 DASRIQ010000000
VERSION     DASRIQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275636"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VFD06_01320"
     CDS             complement(<1..132)
                     /locus_tag="VFD06_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012710388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN02204.1"
                     /translation="MGDALDVVVIGGGHNGLVTAGLLAVGGLKVTVLERRDIVGGACV
                     "
     gene            complement(135..1313)
                     /locus_tag="VFD06_01325"
     CDS             complement(135..1313)
                     /locus_tag="VFD06_01325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN02205.1"
                     /translation="MKRTAEVVIIGGGIQGASVAYHLARRGLTDVVVLEKETLASGCS
                     GRTGGVIRQHYSTPIVTEIARQARAFFAGFDAEVGGHSGFVQNGLVLMVGPKDEEALR
                     ANVALGRAHGVPVQMVDAAEARERVPGLDTTGAVAFAYEADAGYGDGYGTTVAFAARA
                     RDLGVTVLQRTAATGLALEKGRVAGVETPQGSIACKVVVNAAGPWADRVARMAGVDVA
                     LKLELIEEAVIRAPEGEPYPITTPSVYDFVNGLSYRPEGAGQVMAEGNSYYKGSLDPD
                     AYPSQPSDAYIEDVALRLQRSMPRLGGGTVRGGWAGLLEGSPDFHPILGAVEGADGFL
                     LCCGFSGHGFKEAPVTGRLIAELILDGRASLDIAPLAFDRFRRGALLRSNYKDDPIMA
                     "
     gene            complement(1328..>2227)
                     /locus_tag="VFD06_01330"
     CDS             complement(1328..>2227)
                     /locus_tag="VFD06_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HZN02206.1"
                     /translation="KTQPSLKAPPPPKAGLQILLDLIPENPVDAMARAFQGEMLAVMV
                     FALIFGIALTRVERSTAEPLLRWLEALLAAAMAVIAFAMRLAPYGVGAILFTLTARFG
                     IDILKPLALYMGTVILGLALHQFGTYPLVLRVFAGLSPRFFFARMREVMLTAFSTSSS
                     NATLPTTLRVAQEGLGIPRPIASFVLTLGSTLNQNGTALYEGITVLFLAQFFGVHLSL
                     GGQVTVVLLSILAGVGTAGVPGGSLPLVAALLVTVGVPYEGIGIIYGVDRLLDMCRTV
                     LNVTGDVVAAACVARAEQNSPTS"
BASE COUNT          355 a          814 c          761 g          297 t
ORIGIN      
        1 gacgcaggcg ccgccgacga tgtcgcgccg ctcgaggacg gtgaccttga gcccgccgac
       61 cgcgagcagg cccgcggtca cgaggccgtt gtgcccgccg ccaatcacca ccacatcgag
      121 agcgtcgccc atcgtcaggc catgatcggg tcgtctttgt agttcgaccg gaggagcgcc
      181 ccgcggcgga aacgatcgaa agccagaggc gcgatgtcga ggctggcccg tccgtcgagg
      241 atcagctcgg cgatcaggcg tcctgtgacc ggcgcctcct tgaagccgtg gccggagaag
      301 ccgcagcaga ggaggaaccc atcggctccc tcgaccgccc ccaggatcgg gtggaagtcg
      361 ggcgaccctt cgagcagccc ggcccagccg ccgcgcaccg tccccccgcc gagccgcggc
      421 atggatcgct gcagtcgcaa ggccacgtcc tcgatgtagg cgtccgacgg ctgcgacggg
      481 taggcgtccg ggtcgagcga tcctttgtag taggagttgc cctcggccat cacctgaccg
      541 gccccctccg gccggtagga gagcccgttg acgaagtcgt agacgctcgg cgtcgtgatc
      601 gggtacggct ccccttcggg agcccggatg accgcctcct cgatgagctc gagcttcagc
      661 gccacatcca ccccggccat ccgcgccacc cggtccgccc aggggccggc ggcattcacg
      721 acgaccttgc aggcgatcga tccctgcggg gtctcgacgc cagcgacccg ccccttctcc
      781 agcgcgaggc cggtggccgc ggtgcgctgc agcaccgtca cgccgagatc cctggcgcgc
      841 gccgcgaacg ccacggtcgt cccatagccg tcgccgtagc cggcgtccgc ctcgtacgcg
      901 aaggccaccg cccccgtggt gtcgagcccg gggacccgct cgcgcgcctc ggcggcgtcg
      961 accatctgca ccggcacgcc gtgcgcgcga ccgagcgcga cgttggcgcg cagcgcctcc
     1021 tcgtccttgg gcccgaccat gaggacgagg ccgttctgca cgaagccgga atgcccgccg
     1081 acttcggcgt cgaacccggc gaagaaggcg cgcgcctgcc gggcgatctc cgtgacgatc
     1141 ggggtggagt agtgctgccg gatcaccccg cccgtccgcc cgctgcagcc cgaggcgagc
     1201 gtctccttct ccagcaccac gacgtcggtc agcccgcgcc gcgccaggtg gtaggcgacg
     1261 ctcgccccct ggatcccgcc gccgatgatc acgacctccg ccgtgcgctt cacctcaaca
     1321 ccctctttca gctcgttggg ctgttctgct cggcccgcgc cacgcaggca gcggccacga
     1381 catctccggt gacgttgagc accgtccgac acatgtcgag cagccgatcg acgccgtaga
     1441 tgattccgat tccttcatag ggcacgccga cggtgacgag cagcgcggcc acgaggggca
     1501 gcgatccgcc cgggaccccg gcggtgccga cgccggcgag gatcgagagg aggacgacgg
     1561 tcacctggcc gccgagcgac aggtgcaccc cgaagaactg cgccaggaac agcacggtga
     1621 tcccttcgta cagggcggtg ccgttctggt tcagggtcga tccgagcgtc agcacgaagg
     1681 aggcgatcgg ccgcgggatc ccgagcccct cctgcgccac gcgcagcgtc gtcggcaggg
     1741 tcgcgttgct cgacgaggtc gagaaggcgg tgagcatcac ctcccgcatg cgcgcgaaga
     1801 agaagcgcgg cgacaggccg gcgaacaccc ggagcaccag cggataggtc ccgaactggt
     1861 gcagcgccag cccgaggatc accgtcccca tgtagagagc gagcggtttg aggatgtcga
     1921 tcccgaagcg cgccgtcagc gtgaacagga tcgccccgac cccgtagggg gcgagccgca
     1981 tggcgaaggc gatcaccgcc atcgccgcgg ccaggagggc ctcgagccag cgcaggagcg
     2041 gctcggcggt ggagcgttcg acgcgcgtca gcgcgatgcc gaagatcagc gcgaacacca
     2101 tcaccgccag catctccccc tggaaggccc gcgccatggc gtcgaccggg ttctcgggaa
     2161 tgagatcgag gaggatctgc agccccgctt tcggcggcgg cggcgccttg agggacggct
     2221 gcgtctt
//
LOCUS       DASRIQ010000052         8742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_17750, whole genome shotgun sequence.
ACCESSION   DASRIQ010000052 DASRIQ010000000
VERSION     DASRIQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17750"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2647
                     /locus_tag="VFD06_01335"
     CDS             <1..2647
                     /locus_tag="VFD06_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN02207.1"
                     /translation="ARLQRFITGPIGHLATTARQVAEEKNFAVRAHRQGNDEVGLCID
                     AFNEMLAQIQKRDEALKSTHDALEVRVQERTRELRESEMVLRSFYDSTPLMMGVIELA
                     GGDIVHLSDNPSTAAFFGRTPEAMRGRRASEMGVPPDALSMWAERYAESARSGTPVRF
                     EYAHGSGPDSRWLSATVCRIPRSAGGRAQFCYVIEDATERKRAQVELQKAKEAAESAS
                     RAKSEFLANMSHEIRTPLNGVIGMTELLRDSDLSSEQREYLDLVHTSGEALLAVINDI
                     LDFSKIEAGRLDLDPIPFRLRDSLGETMKGLAFRAQSKGLELACHVGAGAPDALVGDP
                     SRLRQVVMNLVGNAIKFTESGEVVVRVEPERSDDGSVDLRFSVQDTGIGIADEKQRTI
                     FEAFTQADGSTTRRYGGTGLGLTISQKLVQLMGGRLWVESAPGSGSTFHFTARFGLQD
                     GRAGLLEPETAVSLSGVRVLVVDDNRTNRWILEEILTQWGLRPVALASGPTALAAASE
                     ARSKGDPYRVALLDVNMPGMDGFETAERLRRDDTDGRTALLLLTSAGRRGDAARCREL
                     GIHGYITKPVNQSDLLDALMTVLDPARGQTPAAALVTRHSLREERRRIRVLLAEDNAV
                     NRRVALGLLEKRGCEVLLAGNGQEALDLLATGPVDVILMDLQMPEMGGLEATAAIRER
                     ERAAGGHVPIIAMTAHAMKGDRERCLAAGMDDYVSKPVRPSDLYAALERALPGSGVPM
                     PLPAVPPATRAAARLVAEAAPGAAARPDAVVDREQLLERLEGDLRLLAEIVTLYHQTC
                     PGLVQELRSALGRHDANGVHRAAHTLKGMVAHFGAPDATRALLDLEEEAREGWSDRLD
                     SAAETAVREIARLDEALDRLRRDAA"
     gene            2708..4261
                     /locus_tag="VFD06_01340"
     CDS             2708..4261
                     /locus_tag="VFD06_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN02208.1"
                     /translation="MDVRLLLDDAARRHEGRPAIHCSGRSLTYREVAAIQDRMAAALD
                     AAGAREGDRVAAFFPNCHLFLAAHYAALRSSLVLMPMNLRLAGPETEAVLRRGGARFL
                     LGAPDRIEPIEAALAAGTSVGRPIAQDDGWRLVPAPTAIGAGAAVPEEGALLYFTSGS
                     TGDPKGVLLTLGNLAAHAEMTLATLGFTPRDVWLHAAPMFHLADAWAIFTATAAGSGQ
                     AFLPRWDGDEALALARSAGVTLTNLVPSMIPSFLDAAGWQAGGVPSLRLLLSGGAPIS
                     PSLPTLIEAVLGCEYAQTYGLTETSPFLTFSLLDEEERRLPPEERRRIQGRTGRPAPG
                     IALRVVVPAGAERFEDVPADDATVGEVVVRGATVTPGYFGDAAATSEAFRGGWFHTGD
                     LATIDARGSIQLVDRAKDIIISGGETIYSVEVERALLRHPEVREAAVFAVPDDRFGEA
                     VRAAVVLGPGGAAGADDLAAFCRTRIAGYKCPRAFDLLAALPRTGSGKIDKRALREPH
                     WRGRPRRIS"
     gene            complement(4266..6212)
                     /locus_tag="VFD06_01345"
     CDS             complement(4266..6212)
                     /locus_tag="VFD06_01345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZN02209.1"
                     /translation="MAQQVASALATGRHLLVEAGTGVGKSLAYLVPAILHAAAGASEE
                     GSRRVVVSTFTRALQEQLARKDLPLLERAFEAQGIAFRHALLMGSENYLCVQRLEQSR
                     RLDAGAVLEALEQHAATAVSGLRSEIPFPVPEPLWARVRRERDVCLGARGPFWERCLY
                     RRDLARAREAEIVVVNHALFFLDLASGGRILPPHGVVVLDEAHRAEEAAAQQFGARLG
                     PGAVTRLLRDIVPPRRGRSEGAARGGRRGRGLRGTPVAGGVRLAAAARQVEEAAVEYF
                     ASARRLAQSPGVRGAAPAAVPLPPGALDRRGLAAAFADLETTLEEAGRETDDLFGRGA
                     LEALRQRAADLRGRLDLFTTQSRQDAVYWIEARGAQEVDLAVSPIEVAPLLRQALFES
                     GRTVVLTSATLSVGGSFAHARDRLGVTASGAEALGSPYDFRRQALLYLPTGMPDPGSD
                     PASWGDAVSAECRRIVEASDGGAFLLFTSFALLDRVHRDLMTEPGMRERPLLRHHPDA
                     PATDLLERFRASRRAVLLGALTFWQGVDVPGEALRAVVVTRLPFEVPDHPLARARAAA
                     ITGRGGDPFLDDSLPEAILTFRQGFGRLIRSHRDFGLVAVLDPRLTSRAYGARFLEAI
                     PPCPRTSSIEEVARFFARAGSETL"
     gene            complement(6329..7177)
                     /locus_tag="VFD06_01350"
     CDS             complement(6329..7177)
                     /locus_tag="VFD06_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02210.1"
                     /translation="MSDSKPPRQALAAILALPDEAIDLGQAALLIARQEYPDLEVGRY
                     LSLLDDMGAEVCRRLRGGEGPMSRIGHLNRYLFDDLGFRGNVEAYDDPRNSFLNDVLD
                     RRVGIPISLSTVYLEVGRRCGWPLDGVSFPGHFLVRWRDHGGDSGEILLDPFHRGRIL
                     TEEECRRRLLEQFGGRLAFRREFLRRARTRDILERTLVNLKRLYEKGQDFHRALRAQD
                     TLLCLSPEDPERLRDRGLLYFRLACFGQACADLEAYLERAPDAADREALRRQMPKLRS
                     LSPVMN"
     gene            7262..8113
                     /locus_tag="VFD06_01355"
     CDS             7262..8113
                     /locus_tag="VFD06_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HZN02211.1"
                     /translation="MPDPTQRRTIQIGHSPDPDDAFMFHGIASGQVPAPDGWDVVQVL
                     RDIESLNRLALEGSIEVTAVSIHAYAYLADRYALLPCGASMGDGYGPMIVAREPLSLE
                     EVRVRTIAVPGDLTSAVLALRLALGEVRTRVVPFDRILAEVREGRADAGLLIHEGQLT
                     YAGEGLHKILDLGAWWKEETGLPLPLGGNAVRKDLGPEVIARVRALLQASIEHGLRHR
                     DEALTYALDFGRGLPRSMADRFVGMYVNDYTLDYGPTGRRAIETFLGRGADAGILPRR
                     VPVEFTA"
     gene            8110..>8742
                     /locus_tag="VFD06_01360"
     CDS             8110..>8742
                     /locus_tag="VFD06_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02212.1"
                     /translation="MSPAAASAAGGGPAAGAYRAAHEEAVLVDRPDLAALRLEGKDAL
                     DLLHRLTTNDVKGLAEGMGAATVFTTAKGRIVALATLHRLKGWLHLLCDAAHAGTIRD
                     WIDRYTFREEVRVEDRSASHATLLVTGPRSEGIVARAFPSAAGLPRHGVVQVDLDGEA
                     GLLVRTFPVASDAWLLTVPGAFAASARARLLAAGAGRLMTAPLEVFEALRI"
BASE COUNT         1213 a         3202 c         3189 g         1138 t
ORIGIN      
        1 cgcccggctg cagcgcttca tcaccgggcc gatcggccat ctcgccacga ccgcccggca
       61 ggtggccgag gagaagaact tcgcggtccg cgcccaccgc caggggaacg acgaggtcgg
      121 cctgtgcatc gacgccttca acgagatgct ggcgcagatc cagaagcggg acgaggcgct
      181 caagtcgacg cacgacgccc tcgaggtgcg ggtgcaggag cggacgcggg agctgcgcga
      241 gagcgagatg gtcctgcggt ccttctacga cagcacgccg ctgatgatgg gggtgatcga
      301 gctcgccggc ggcgacatcg tccacctgtc ggacaatccc tccaccgccg ccttcttcgg
      361 ccgcaccccc gaggcgatgc gcgggcgccg ggcgagcgag atgggcgtgc cgcccgacgc
      421 gctgagcatg tgggccgaac gttatgccga gtcggcgcgc tccggcaccc cggtgcgctt
      481 cgagtacgcg cacggatcgg ggccggactc ccgctggctg tcggccaccg tctgccgcat
      541 cccgcgcagc gccggcggcc gcgcgcagtt ctgctacgtg atcgaagacg cgaccgagcg
      601 caagcgggcc caggtggagc tgcagaaggc gaaggaggcg gccgaatcgg cgagccgcgc
      661 gaagagcgag ttcctggcga acatgagcca cgagatccgc accccgctca acggcgtcat
      721 cggcatgacc gagctcctgc gggacagtga cctctcgagc gagcagcgcg agtacctcga
      781 cctcgtgcac acctcgggcg aggcgctgct cgcggtgatc aacgacatcc tcgacttctc
      841 gaagatcgag gccggccggc tcgatctcga tccgattccg ttccgcctgc gcgacagtct
      901 cggggagacg atgaaggggc tggccttccg cgcgcaatcg aaagggctgg agctcgcctg
      961 ccacgtcggc gccggcgcgc ccgacgcgct cgtcggagac ccgagccgcc tgcggcaggt
     1021 ggtgatgaac ctggtcggca acgccatcaa gttcaccgag tcgggggagg tggtggtgcg
     1081 ggtggagccc gagcgctccg acgacgggtc cgtcgatctg cgcttctcgg tgcaggacac
     1141 cggcatcggc atcgccgacg agaagcagcg gacgatcttc gaggcgttca cccaggcgga
     1201 cggatcgacg acgcgccgct acggcggcac cggcctcggg ctgacgatct cccagaagct
     1261 cgtgcagctg atgggaggaa ggctgtgggt cgagagcgcc cctggcagcg gcagcacgtt
     1321 ccacttcacc gcccgcttcg gcctgcagga cgggcgcgcg ggactgctgg agccggagac
     1381 cgcggtcagc ctctctgggg tgcgggttct cgtggtggac gacaaccgca ccaaccggtg
     1441 gatcctcgag gagatcctga cgcagtgggg gctccggccg gtggccctcg cctccggccc
     1501 caccgccctc gcggccgcct ccgaagcgcg gtccaagggg gacccgtacc gcgtcgcgct
     1561 cctggacgtc aacatgccgg ggatggacgg cttcgagacc gcggagcggc tgcgccgcga
     1621 cgataccgac gggcgcaccg ccctcctgct cctcacctcc gccgggcggc gcggcgacgc
     1681 ggcccgctgc cgcgagctcg ggatccacgg ctacatcacc aagccggtga accagtccga
     1741 cctcctcgac gcgttgatga ccgtcctcga cccggcgcgc gggcagactc cagctgcggc
     1801 cctcgtgacg cggcactcgc tgcgcgagga gcgtcggcgg atccgggttc ttctggcgga
     1861 ggacaacgcc gtcaaccggc gcgtcgccct gggcctcctc gagaaacgcg gctgcgaggt
     1921 gctcctcgcc ggcaatggcc aggaggcgct cgatctgctc gcaaccggtc cggtggacgt
     1981 gatcctcatg gacctgcaga tgccggagat gggggggctc gaggccacgg ccgcgatccg
     2041 cgagcgggag cgcgcggccg gcggacatgt gccgatcatc gcgatgaccg cccacgccat
     2101 gaagggggac cgggagcgct gcctcgcggc aggcatggac gactacgtca gcaaaccggt
     2161 gcggccgtcc gatctgtacg ccgcgctgga gcgggcgctt cccggaagcg gcgtcccgat
     2221 gccgctcccg gcggtcccgc ccgcgacgcg agcggcggcg cggttggtgg ccgaggcggc
     2281 gcccggagcg gccgcgcgac ctgacgcagt cgtggaccgc gagcagctcc tcgagcgcct
     2341 cgaaggggac ctgcgcctcc tggcggagat cgtcacgctg taccaccaga cctgccccgg
     2401 cctggtgcag gagctgcgct cggcgctcgg tcgccacgac gcgaacggcg tccatcgcgc
     2461 cgcgcacacg ctgaagggca tggtggccca tttcggggcg ccggacgcga cccgcgccct
     2521 gctcgatctc gaggaggagg cgcgcgaagg gtggtccgat cgcctcgatt ccgctgcgga
     2581 gacggccgtg cgcgagatcg cgcggctgga cgaggccctc gatcggctgc ggcgcgacgc
     2641 cgcctgatct tccgccgcgg tgccgcctga cgccccggcg cggcgtcacc ttggatacca
     2701 tgccggcgtg gacgtccgcc tgctgctcga cgacgccgcc cgccgccacg aagggcgccc
     2761 cgcgatccac tgctccggcc ggtcgctgac ctaccgtgag gtcgcggcga tccaggatcg
     2821 gatggcggcg gcgctcgacg ccgccggggc gcgggaaggg gacagggtcg cagccttctt
     2881 cccgaactgc cacctctttc tcgccgccca ttacgccgcg ctccgctcca gcctcgtcct
     2941 gatgccgatg aacctccgtc tcgcgggccc cgagaccgag gcggtgctgc ggcgcggcgg
     3001 agcgaggttc ctgctcggcg cgccggaccg gatcgagccg atcgaagcgg cgctcgccgc
     3061 cggcacgtcc gtgggccggc cgatcgccca ggacgacggc tggagactcg tcccggcgcc
     3121 gaccgcgatc ggcgccggcg ccgcggttcc cgaggagggg gcgcttctct atttcaccag
     3181 cggcagcacc ggcgatccca agggagtcct cctgaccctc gggaatctcg cggcgcacgc
     3241 ggagatgacc ctcgcgaccc tcggcttcac gccgcgggac gtgtggctgc acgccgcccc
     3301 gatgttccat ctcgccgacg cctgggcgat cttcaccgcc accgccgcgg gctcggggca
     3361 ggcgttcctt ccgcgctggg acggcgacga ggcgctggcc ctggcgcgct cggcgggcgt
     3421 gacgctcacc aacctcgtgc cgtcgatgat cccgtccttc ctcgacgccg ccgggtggca
     3481 ggcgggcggc gtcccctcgc tgcggctcct cctgagcggc ggcgcgccga tttcaccgtc
     3541 gcttcccacc ctcatcgaag cggtgctcgg ctgcgagtac gcgcagacct acggcctcac
     3601 cgagacctcg ccgttcctca ccttctcgct cctcgacgag gaggagcgcc gcctgccgcc
     3661 ggaggagcgg cgccgcattc aggggcggac cggccgcccg gcccccggga tcgccctgcg
     3721 ggtggtcgtc cccgcggggg cggagcggtt cgaggatgtc ccggcggacg acgccacggt
     3781 gggggaggtg gtggtgcgcg gggccaccgt gacccccgga tatttcggcg acgcggcggc
     3841 gacgagcgag gcgttccgcg gcggctggtt ccacaccggc gatctcgcga ccatcgacgc
     3901 gcgcggctcg attcagctcg tcgatcgcgc caaggacatc atcatcagcg gtggcgagac
     3961 gatctacagc gttgaggtgg agcgggccct gctccggcac ccggaggtgc gggaggcggc
     4021 ggtcttcgcc gttcccgacg atcgtttcgg cgaggcggtg cgggctgcgg tcgtcctcgg
     4081 ccccggcggc gccgccggcg ccgacgatct cgccgccttc tgccgcaccc ggatcgccgg
     4141 ctacaaatgc ccgcgcgcct tcgatctcct cgccgcgctg ccgcgcaccg gctccgggaa
     4201 gatcgacaag cgggcgctcc gggagccgca ctggcgcggt cgcccgcggc gcatctccta
     4261 ggagcctaga gcgtttccga tccggcgcgc gcgaagaagc gcgccacctc ctcgatcgac
     4321 gaggtccgcg ggcacggcgg gatcgcctcg aggaagcgcg ctccgtaggc gcggctcgtc
     4381 aggcgtggat cgagcacggc gacgagcccg aaatcgcggt gcgagcggat gagccgcccg
     4441 aacccctgcc ggaacgtcag aatcgcctcc ggcaggctgt cgtcgaggaa cgggtcgccg
     4501 ccgcggcccg tgatggcggc ggcgcgcgcc cgtgccagcg ggtggtccgg cacctcgaac
     4561 ggcagccggg tcaccaccac ggcgcgcaac gcctcccccg gcacgtccac tccctgccag
     4621 aaggtcagcg ccccgaggag cacggcacgg cgcgaggcgc ggaagcgctc gaggaggtcg
     4681 gtcgcgggag catccgggtg gtgccggagg agcggccgct cgcgcatccc cggctccgtc
     4741 atgaggtccc ggtgcacgcg atcgaggagc gcgaaggagg tgaagagcag gaacgccccg
     4801 ccgtccgagg cttcgacgat gcggcggcac tcggcgctca ccgcgtctcc ccaggaggcc
     4861 ggatcggatc cgggatccgg catcccggtc ggaaggtaga ggagcgcctg gcgccggaaa
     4921 tcgtacggcg acccgagcgc ctcggcgccc gacgcggtga cccccagccg gtcgcgggcg
     4981 tgcgcgaacg atcccccgac cgacagcgtc gccgaggtca gcacgacggt gcgccccgat
     5041 tcgaagagcg cctggcgcag caggggcgcg acctcgatcg gcgacaccgc gagatcgacc
     5101 tcctgcgcgc cgcgggcctc gatccagtag accgcgtcct ggcgcgactg cgtggtgaac
     5161 agatcgaggc gcccgcgcag atcggccgcc cgctgccgca gcgcctccag ggcgccgcgg
     5221 ccgaacaggt cgtcggtctc ccgtcccgcc tcctcgagcg tcgtctcgag gtcggcgaag
     5281 gcggcggcga ggccgcggcg atcgagcgcg cccggcggca gcgggaccgc cgccggcgcc
     5341 gcccccctga cgccggggct ctgcgcgagc ctccgggcgg aggcgaagta ctccaccgcc
     5401 gcctcctcca cctgccgcgc cgccgcggcg aggcggaccc cgcccgcgac cggcgtcccc
     5461 cgcaggccgc gcccgcgacg gccgccgcgc gcggcccctt cggaccggcc gcggcgcggg
     5521 gggacgatgt cgcgcaggag ccgcgtcacc gcaccgggac cgaggcgggc gccgaactgc
     5581 tgagcggcgg cctcctccgc ccggtgcgcc tcgtccagca ccacgacacc gtgcggcggc
     5641 aggatccgcc cgccgctcgc gagatcgagg aagaacaagg cgtggttcac cacgacgatc
     5701 tccgcctcgc gggcccgcgc cagatcgcgg cggtacaggc accgctccca gaacgggccg
     5761 cgcgcgccga ggcagacatc ccgctcgcgc cgcacgcgcg cccacagcgg ctccggcacc
     5821 gggaagggga tttcggatcg cagccccgac acggcggttg cggcgtgctg ctccagcgct
     5881 tcgaggacgg cgccggcgtc gaggcgcctc gactgctcga ggcgctgcac gcagaggtag
     5941 ttctccgagc ccattaggag cgcgtgacgg aaggcgatcc cctgcgcctc gaaggcccgc
     6001 tccaggagcg gcaggtcctt gcgggccagc tgctcctgga gcgcgcgggt gaaggtggag
     6061 acgacgacgc ggcgcgaccc ctcttcggag gcgccggcgg cggcgtgcag gatcgccggg
     6121 acgaggtatg cgagcgactt gccgaccccg gtgcccgcct ccaccaggag gtgccgaccc
     6181 gtcgcgaggg ccgatgcgac ctgttgcgcc atcgcgacct gctgggggcg cggctcgtag
     6241 cccgagagcg cgcgcgccag cgggccctcc gggccgagga acgactcgat ggtcgggccg
     6301 ggcgccgccg aggccggccc cgcgggactc agttcatcac cggcgacagg gagcgcagct
     6361 tcggcatctg ccggcgcagc gcctcccggt cggccgcgtc cggagcgcgc tcgaggtagg
     6421 cctcgagatc ggcgcaggcc tgcccgaagc aggccaggcg gaaatacagc agcccccgat
     6481 cgcgcagccg ctccggatcc tcggggctga gacacaggag cgtgtcctgc gcccggaggg
     6541 cgcgatggaa gtcctgcccc ttctcgtaca ggcgcttcag attgacgagc gtccgctcca
     6601 ggatgtcgcg ggtccgcgcc cggcgcagga actcgcgccg gaaggccagc cggccgccga
     6661 actgttccag gagccgccgg cggcactcct cctccgtcag gatgcggccg cgatggaacg
     6721 gatcgagcag gatctccccg ctgtcccccc cgtggtcgcg ccagcggacg aggaaatgcc
     6781 ccgggaagga gacgccgtcg agcggccagc cgcagcgtcg cccgacctcg aggtagaccg
     6841 tcgacaggct gatcggaatg ccgacgcgcc gatcgaggac gtcgttcagg aaggaattcc
     6901 gcggatcgtc gtacgcctcg acgttgccgc ggaatcccag gtcgtcgaac agataccggt
     6961 tcaggtggcc gatccggctc atcggcccct ccccgccgcg aagcctccgg cacacctccg
     7021 cccccatgtc gtcgagcagg gagagatagc gtcccacctc caggtccggg tactcctggc
     7081 gggcgatcag gagggccgcc tggcccaggt cgatcgcctc gtccggcagc gcgaggatgg
     7141 ccgcgagggc ctggcgcggt ggtttggagt cgctcatcga agggcccgtc acagggggcc
     7201 gcctatcata tccaccgcgg gccattttcc ctataatgtg cggtttcaaa cggagggtcc
     7261 gatgcccgac ccgacgcagc gccgcaccat ccagatcggc catagccccg accccgacga
     7321 cgcgttcatg ttccacggga tcgcttcggg acaggtcccg gcgccggacg gctgggacgt
     7381 ggtccaggtg ctgcgcgaca tcgagtcgct caatcgtctg gcgctggaag ggtcgatcga
     7441 agtgaccgcc gtgtcgatcc acgcctacgc ctatctcgcc gaccgctatg cccttctgcc
     7501 gtgcggggcc agcatggggg acggctacgg cccgatgatc gtggcgcgcg agccgctgtc
     7561 gctcgaagag gtgcgcgtca ggacgatcgc ggtgccgggg gacctcacct ccgccgtcct
     7621 ggcgctgcgg ctggcgctcg gagaggtccg gacgcgggtc gtcccgttcg atcgcatcct
     7681 cgcggaggtg cgggaggggc gcgcggacgc ggggctcctc atccacgagg ggcagctcac
     7741 ctacgccggc gaggggctgc acaagatcct cgatctcggg gcgtggtgga aggaggagac
     7801 cgggctgccg ctgccgctcg gcggcaacgc cgtgcgcaag gatctcgggc ccgaggtgat
     7861 cgcccgggtg cgcgcgctcc tgcaggcgag catcgagcac ggcctccgac accgggacga
     7921 ggcgctgacc tacgcgctcg acttcgggcg cgggctgccg cgctccatgg ccgatcgctt
     7981 cgtcgggatg tacgtcaacg actacacgct cgactacggt ccgaccggac gccgcgccat
     8041 cgagacgttc ctcggccgcg gcgccgacgc cgggatcctg ccccggcgcg tgccggtcga
     8101 gttcaccgca tgagcccggc cgccgcgtcg gccgccgggg gtgggcccgc ggcgggagcg
     8161 tatcgggccg cccacgagga ggcggtcctc gtcgatcgtc ccgacctcgc cgcgctgcgc
     8221 ctcgagggga aggacgctct cgacctgctg caccgcctga cgacgaacga cgtgaagggg
     8281 ctggccgagg ggatgggcgc ggcgaccgtc ttcaccaccg ccaaggggcg catcgtcgcc
     8341 ctcgccacgc tgcaccggct gaagggctgg ctgcacctcc tgtgcgacgc cgcgcacgcc
     8401 gggacgatcc gggactggat cgatcgctac accttccgtg aggaggtccg ggtggaggat
     8461 cggagcgcga gccacgcgac gctgctcgtc accggcccgc gatcggaggg aatcgtcgcg
     8521 cgcgccttcc cctccgccgc cgggcttccc cgtcacggcg tcgtgcaggt cgatctggac
     8581 ggcgaggccg ggctcctcgt gcgcacgttc ccggtcgctt cggatgcctg gctcctgacc
     8641 gtgccgggcg ctttcgcggc ttcggcgcgc gcgcgcctgc tcgccgccgg cgcggggcgc
     8701 ctgatgaccg cgccgctcga ggtgttcgag gcgctgcgga tc
//
LOCUS       DASRIQ010000053         6308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_17895, whole genome shotgun sequence.
ACCESSION   DASRIQ010000053 DASRIQ010000000
VERSION     DASRIQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17895"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VFD06_01365"
     CDS             <1..1337
                     /locus_tag="VFD06_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HZN02213.1"
                     /translation="PAYFGVGYIIGPELAALNFSGGVIAWGLMVPLITYLLGPQLAQF
                     MPAGTDIEGWGGTITAVWRFIVRPIAVGGMLVGTAYTLFMRRTSIFGGLAKAFAEVRG
                     KGAAVQQAGRTEQYMSSKVIFALIALFFFLMIALYMYMTGLVGGAIVAALVMLVVGFF
                     FATVSGYLVGLIGSSNNPISGLTLSTLVIAALLMVGMGVAGIGGVAAVLGVAAVVCVS
                     SAVAGELLQDFKVGYILGGTPRTIQIVELVAVVAASLVMYFPLLVLHQGNINAGGTGF
                     GDRQLSAPQAGLMAALAQGIVGGEMAWPLIVVGIMMGISMIMVKVKSPMLVAVGMYLP
                     LATTSAIFVGGVIRWFVDRFATRRGLNAAQRVRVENVGILAASGLIAGEALVGLGTAT
                     CNFFNIPIPGVFESPSYVAGLVVVALVVYLLVKVPLDNAGRADEPAPPSAMM"
     gene            1351..2775
                     /locus_tag="VFD06_01370"
     CDS             1351..2775
                     /locus_tag="VFD06_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN02214.1"
                     /translation="MAPEANPPVAASAAPVARLRRELGLWDLVLLNIVAIVGLRWWLT
                     SAGGYGYAALPLWILAFVCFFVPSGLAVIDLTTRYPEEGGIYAWTKHAFGEKHGFISG
                     WCLWTNNLIYFPHLLIFTVGNLVFMFGKEHEGLKSSPPFMAAVSLLIFWVVVWMNVLG
                     LKRARWLNNLGAYGTWLPATVLILLGGMALWKYGPATPLEAGGFIPIFSLGTVSFFSA
                     ICFGFSGLDLGALMNEEIVEPRRNVPRAIFISGIVITLIYVLGTAALLVALPQGNIGL
                     LDGVGAAIAAVQEKMGFGFLAGIMALLIALGGVGGCSAWLAGCSRIPFVAGIDRYLPP
                     AFGSLHPRHGTPHVALLVTGAISSLLILVGFVGVDVEEAYLFLADFTIVVYFIPYCYL
                     FAALLKLGGGTTGEGMIPVPGGRTGAILVGTVGFLTTAVAIAFALIPPKGTDALLFEA
                     KILGGCALLLGCGWVLYLRGRRKA"
     gene            2783..3523
                     /locus_tag="VFD06_01375"
     CDS             2783..3523
                     /locus_tag="VFD06_01375"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZN02215.1"
                     /translation="MADRFPTTHWSAVRGAQSAEPEERRRSLEAIAAAYWRPVYKHVR
                     LSRRRSPEDAEDLTQAFFARAFEKEFFAGYDPAKGRFRTFLRTCVDRFVANEDKAAAR
                     LKRGGGAAALPCDFEAAEAEIAAAGARGAGRSPEDLFDDEWVRSLFGLAVEALRQECE
                     AAGKGRHFDLFRRYDLEDAGAGGITYDDLAREFSLTTSLVTNHLAWARREFRRLLLET
                     LRGVTGSDEEFRLEARLLLGSEPPRHAG"
     gene            3513..4580
                     /locus_tag="VFD06_01380"
     CDS             3513..4580
                     /locus_tag="VFD06_01380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN02216.1"
                     /translation="MLDDERLAHLRAVVDLPDLTGTRYRLVHRLGAGGMATVYLVVDE
                     SLGREAALKVLTDPDPGGQMAARLVEEARLLAGLEHPNLAPVHDVGTLPDGRVFYVMK
                     YVRGARLDAWIASVTDRPAILRLFVKVCDAVAFAHAHGVVHRDLKPQNIMVGAFGEAL
                     VMDWGVAKRLSAPEAPTLQLDAPAAPGIAPGRPAGAAPVGTRAGTVIGTPAWMAPEQA
                     RGDLDQVGPRTDVYALGAILYYLLSGRPPFPAGPAESVLRQVREESPADLRRLDASIP
                     KRLAAIVRRAMAKDPGARYAAASDLSADVLRHLDGLAVAAYPESFLERGLRVLSRTRT
                     LAALIAAYIAMRLILLWFTGR"
     gene            complement(4577..6220)
                     /locus_tag="VFD06_01385"
     CDS             complement(4577..6220)
                     /locus_tag="VFD06_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02217.1"
                     /translation="MDVRRKHLLHAVMILLALAGYLFALNYRRDEVPRGLNNDVAEEG
                     LRGIQLLEAKRLEVINAHGVNADGRHFGHSMETLYLYLVGFAAEVLGSTSLAVHATTW
                     GFTLASIVLLCAVVRRLRPGMPVAIPLLLAVSSVWLFHYGRSGLRANTAPLFMLAFAL
                     ALDSVERDERPRFWKATACGGILGAGLYGYTACRVLPVAFLLYVALSMLRGHGALRGL
                     KIGSAVALGALVVSIPNLVYLAENGREFLTRGAYVLPAHLPDAARNLLHSLALPLFYP
                     WYSKAVDATHHFDGVSFGLTVAGLRPIHPLIGLAIVAGVVQAWRLRRTPIVSLLFSIW
                     TVSLLALGISGPSLTRFLIVLPVFLVVAALGIEPLLARPWTRRATVAALCLLAAAQAS
                     DYFVRLSRDPKSAREFAEAATDIGRRARALAGTDRQVLCIVAGDANVVHYLTYDGGRK
                     VAIKEFWHRRPDPREIPFQRVAPEVILVERHPDLAFLHDAFLPLGRRETHPLFDEFFV
                     DPAWDWQTLSLKEGPDSPLEKPVAGYRSDTAATTIQVIL"
BASE COUNT          886 a         2193 c         2209 g         1020 t
ORIGIN      
        1 cccccgcata cttcggcgtc ggctacatca tcgggccgga gctggcggcg ttgaacttct
       61 ccggcggcgt catcgcctgg gggctgatgg tccccctcat cacctacctg ctcgggccgc
      121 agctcgccca gttcatgccg gcgggcaccg acatcgaggg ctggggtggg accatcaccg
      181 ccgtctggcg cttcatcgtc cggccgatcg ccgtgggagg catgctcgtc ggcacggcct
      241 acacgctctt catgcggcgc accagcatct tcggagggct ggcgaaggcc ttcgccgagg
      301 tcaggggcaa gggcgctgcc gtgcagcagg cggggcgcac cgagcagtac atgtcctcca
      361 aggtcatctt cgcgctcatc gcgctgttct tcttcctgat gatcgccctc tacatgtaca
      421 tgaccgggct cgtcggcggg gcgatcgtcg cggcgctcgt gatgctggtc gtcgggttct
      481 ttttcgccac cgtctcgggg tacctcgtcg gtctcatcgg atcgtccaac aacccgatct
      541 ccgggctgac cctctcgacg ctcgtcatcg ccgccctgct gatggtcggc atgggggtcg
      601 ccggaatcgg cggggtggcg gcggtgctcg gcgtcgcggc ggtcgtctgc gtgtcgtcgg
      661 cggtggcggg ggagctgctc caggacttca aggtcgggta catcctcgga ggcacgccgc
      721 gcaccatcca gatcgtcgag ctggtcgcgg tcgtcgccgc cagcctggtg atgtacttcc
      781 cgctcctcgt cctgcaccag gggaacatca atgccggcgg gaccggattc ggagaccggc
      841 agctgtcggc gccgcaggcg gggctgatgg cggcgctcgc acaggggatc gtcggcggtg
      901 agatggcgtg gccgctcatc gtcgtcggca tcatgatggg catctcgatg atcatggtga
      961 aggtgaagag cccgatgctc gtggcggtcg ggatgtacct gccgctcgcc accacctcgg
     1021 cgatcttcgt cggcggcgtc atccggtggt tcgtcgatcg cttcgcgacg cgccgcggcc
     1081 tgaacgccgc gcagcgggtc cgggtggaga acgtcggcat cctcgctgcg tccggactca
     1141 tcgccggcga ggcgctcgtc ggcctcggca ccgccacctg caacttcttc aacattccga
     1201 tccccggggt cttcgagtcg ccgtcctacg tcgccggcct cgtcgtggtc gcgctcgtcg
     1261 tgtacctcct ggtgaaggtg ccgctcgaca acgccgggcg cgccgacgaa ccggcgccgc
     1321 cttcggcgat gatgtagccg ggcatcacgg atggcccccg aagcgaatcc gccggtcgcc
     1381 gcgtccgcgg cgccggtcgc gcgcctgcgg cgcgagctgg gcctctggga cctcgtcctc
     1441 ctcaacatcg tcgcgatcgt cggcctgcgc tggtggctga cgtccgcggg cgggtatggc
     1501 tacgcggcgc tgccgctgtg gatcctggcg ttcgtctgct tcttcgtccc gtccgggctg
     1561 gcggtcatcg atctcacgac gcgctacccg gaagaggggg ggatctacgc ctggaccaag
     1621 cacgcgttcg gcgagaaaca cggcttcatc tccggctggt gcctgtggac caacaacctc
     1681 atctacttcc cacacctgct gatcttcacg gtcggcaatc tcgtgttcat gttcggcaaa
     1741 gagcacgaag ggctgaagtc gagcccgccg ttcatggcgg cggtgtcgct gctgatcttc
     1801 tgggtggtcg tctggatgaa cgtcctgggg ctgaagcggg cgcgctggct gaacaacctc
     1861 ggggcctacg gcacctggct gccggcaacg gtgctgatcc tcctcggggg catggcgctc
     1921 tggaaatacg gccccgccac gcccctcgag gcgggcggct tcatccccat cttcagcctc
     1981 ggaacggtct ccttcttctc ggcgatctgc ttcggtttct ccggcctcga cctcggggcg
     2041 ctgatgaacg aggagatcgt cgagccgcgc cgcaacgtgc cgcgcgccat cttcatctcc
     2101 ggcatcgtca tcaccctcat ctacgtcctc gggaccgcgg cgctgctggt cgcgctgccc
     2161 caaggcaaca tcgggttgct cgacggcgtc ggcgcggcga tcgcggcggt gcaggagaag
     2221 atggggttcg ggttcctcgc ggggatcatg gcgctgctca tcgcgctcgg gggggtcggg
     2281 ggctgcagcg cctggctcgc cggctgttcg cgcatcccgt tcgtcgcggg gatcgaccgt
     2341 tacctccccc cggcgttcgg ctcgctgcac ccgaggcacg gcacgccgca cgtggcgctc
     2401 ctcgtgaccg gcgccatctc gagcctcctc atcctcgtcg gcttcgtcgg ggtggacgtc
     2461 gaagaggcgt acctcttcct cgccgacttc accatcgtcg tgtacttcat cccgtactgc
     2521 tacctgttcg ccgccctgtt gaagctgggc ggaggcacga cgggggaagg gatgatcccg
     2581 gtccccgggg ggcgaacggg agcgatcctc gtcgggacgg tcggcttcct gacgacggcg
     2641 gtcgccatcg ccttcgcgct gatcccgccg aaggggaccg acgcgctgct gttcgaggcg
     2701 aagatcctcg gcggctgcgc cctgctgctc ggctgcggct gggtcctgta tctcagaggg
     2761 cgccgcaagg cgtgagcgcg ccgtggccga ccgcttcccg acgacgcact ggtcggcggt
     2821 ccgcggcgcg cagagcgccg agccggagga gcgccgccgc tcgctcgagg cgatcgccgc
     2881 ggcctactgg cgcccggtct acaagcacgt ccggctgagc cggcgtcgat cccccgagga
     2941 cgccgaggac ctgacgcagg cgttcttcgc gcgcgcgttc gagaaggagt tcttcgccgg
     3001 ctacgatccg gcgaaggggc gcttccgcac cttccttcgc acctgtgtcg atcgcttcgt
     3061 cgccaacgag gacaaagccg ccgcgcgcct gaagcggggg ggcggcgcgg cggcgctgcc
     3121 ctgcgacttc gaggcggccg aggcggagat cgccgcggcc ggcgcccgcg gcgccgggcg
     3181 atcccccgag gatctcttcg acgacgaatg ggtgaggagc ctcttcggcc tcgccgtcga
     3241 ggcgctgcgc caggagtgcg aggcggccgg gaagggccgt catttcgatc ttttccggcg
     3301 ctacgatctg gaggatgcgg gggccggagg gatcacctac gacgatctgg cgcgcgagtt
     3361 ctccctcacg acgagcctcg tgaccaacca tctcgcctgg gcccggcgcg aattccgccg
     3421 cctgctgctc gaaacgctgc gcggcgtcac cggcagcgac gaggagttcc gcctcgaggc
     3481 gcgcctcctg ctgggatccg agccgccgcg ccatgctgga tgacgaacgg ctggcgcacc
     3541 tgcgcgccgt cgtggatctt cccgacctga ccgggacgcg ctaccggctc gtccaccgcc
     3601 tcggggccgg cgggatggcg accgtctacc tcgtggtgga cgaatcgctc gggcgggagg
     3661 cggcgctcaa ggtcctcacc gatccggacc cgggcgggca gatggcggca cgcctcgtgg
     3721 aggaggcgcg cctgctcgcc ggcctcgagc acccgaacct cgcgccggtg cacgacgtcg
     3781 gcacgctccc cgacggccgc gtcttctacg tcatgaagta cgtgcgcggc gcccgcctcg
     3841 acgcctggat cgcctcggtc accgaccgcc cggcgatcct gcgcctcttc gtgaaggtct
     3901 gcgacgccgt cgccttcgcg cacgcgcacg gcgtcgtgca ccgcgatctc aagccgcaga
     3961 acatcatggt gggagcgttc ggcgaggcgc tggtgatgga ctggggcgtc gccaagaggt
     4021 tgagcgctcc cgaagctccg acgctgcagc tggacgcgcc cgccgctccc ggcatcgcgc
     4081 caggtcgacc cgccggtgcg gctcccgtcg ggacgcgggc gggcaccgtc atcggcacgc
     4141 ctgcctggat ggcgccggag caggcacgcg gcgacctcga tcaggtgggg ccgcgcaccg
     4201 acgtctacgc tctgggcgcc atcctctatt acttgttgtc cggccgtccg cccttccccg
     4261 caggtcccgc agaatcggtt ctccggcagg tgagggagga gtcgccggcg gatctgcggc
     4321 gcctcgacgc gtcgatcccg aagcggctcg ccgcgatcgt ccggcgcgcc atggcgaagg
     4381 acccgggtgc gcggtatgcc gcggcgtccg atctctccgc cgacgtcctg cggcatctcg
     4441 acggcctcgc cgtcgcggcc taccccgaat cgttcctgga gcgcgggctc cgagtcctga
     4501 gccggacccg cacgctggcc gctctcatcg ctgcgtatat cgcgatgcgc ctgatccttc
     4561 tctggttcac cggccgctag agaatgacct ggatcgtggt tgccgcggtg tcggagcggt
     4621 agccggcgac aggcttttcg agcggcgagt cggggccttc cttgagggac agcgtctgcc
     4681 agtcccaggc gggatcgacg aagaactcgt cgaagagcgg gtgcgtctcg cggcgcccga
     4741 gcggcaggaa cgcgtcgtgg aggaacgcca gatcgggatg gcgctcgacg aggatcacct
     4801 cgggcgccac gcgctggaac gggatctcgc gggggtccgg ccgccggtgc cagaactcct
     4861 tgatggcgac cttgcggccg ccgtcgtagg tcaggtagtg cacgacgttg gcgtcgccgg
     4921 cgacgatgca cagcacctgc cggtcggtcc cggcgagcgc gcgcgcccgg cggccgatgt
     4981 cggtggccgc ctcggcgaac tcgcgcgccg acttcgggtc gcggctcagg cggacgaagt
     5041 aatcggaggc ctgcgccgcc gccaggaggc acagagccgc caccgtcgcg cggcgcgtcc
     5101 acgggcgcgc cagcagcggc tcgatcccga gcgcggcgac gacgaggaag accggcagca
     5161 cgatcaggaa gcgggtcagg ctcggcccgg agatgccgag cgccaggagg ctcaccgtcc
     5221 agatcgagaa gagcagcgag acgatcggcg tgcgccgcag ccgccacgcc tgcaccaccc
     5281 cggcgacgat cgccagaccg atgagcggat ggatcggccg caggccggcg accgtcagtc
     5341 caaacgagac accgtcgaag tgatgcgtcg cgtccaccgc cttcgagtac cacggataga
     5401 agagcggcag cgccagcgaa tggaggaggt tgcgggcggc atccggcagg tgggccggaa
     5461 ggacgtaggc gccgcgcgtc aggaactcgc gtccgttctc cgccaggtag acgaggttcg
     5521 ggatcgagac gaccagcgcc cccaacgcca ccgcggagcc gatcttcagg cctcggagcg
     5581 ccccgtggcc gcgcagcatc gacagggcga catacaggag gaaggcgacc ggcaggacgc
     5641 ggcaggcggt gtagccgtag agcccggccc cgaggattcc tccgcaggcg gtcgccttcc
     5701 agaagcgcgg gcgctcatcc cgttcgacgc tgtcgagcgc cagggcgaat gccagcatga
     5761 acagcggcgc ggtgttggcc cgcagacccg atcgtccgta gtggaagagc cacaccgaag
     5821 agacggcgag cagcaacggg atcgcgaccg gcatacccgg ccggaggcgg cggaccacgg
     5881 cgcacaacag gacgattgac gcgagcgtga agccccaggt cgtcgcgtgc accgccaggc
     5941 tcgtgcttcc caggacctcc gcggcgaacc cgacgaggta gaggtacagc gtctccatcg
     6001 agtggccgaa gtgccgcccg tcggcgttga ctccgtgcgc gttgatcacc tcgagccgct
     6061 tcgcctcgag gagctggatg ccgcgcagcc cctcctccgc cacgtcgttg ttcaatcccc
     6121 gcgggacctc gtccctccgg tagttcagag cgaacagata cccggcgagc gccagcagga
     6181 tcatcacggc gtgcaggaga tgtttgcgac gcacgtccat cccggcctcc ctgaaaaccc
     6241 ctgagggata acgcattcac ggggttgtaa ggttgcaaca atcccgtgtc ggattcgcgc
     6301 cgccgtgc
//
LOCUS       DASRIQ010000054         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2630223, whole genome shotgun sequence.
ACCESSION   DASRIQ010000054 DASRIQ010000000
VERSION     DASRIQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630223"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFD06_01390"
     CDS             <1..511
                     /locus_tag="VFD06_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN02218.1"
                     /translation="ADVRPWREGGTGGDASRPLQPEEFPHRLEGGTPNVFGIAGLRAG
                     LRWVLERGPEAILAHERRLLRGFLAALKRADAFAWYGADRALLDRGGEGRVGLVAFNA
                     RGTPPQAIAAALDERFGIAVRPGLHCAPYAHRHLGTFPYGAVRISVGALTRPEELEAA
                     AAALDQLAG"
     gene            593..715
                     /locus_tag="VFD06_01395"
     CDS             593..715
                     /locus_tag="VFD06_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02219.1"
                     /translation="MRNTPISVLARAATFGVPHSNECPHLTTYPRKIKHLHDDT"
     gene            811..2187
                     /locus_tag="VFD06_01400"
     CDS             811..2187
                     /locus_tag="VFD06_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN02220.1"
                     /translation="MPAVQEDPATPSAGIREEMERQVAAWSAKTGKSREAWERARRNV
                     PGGVSGNFRLMNPYPMFAARAKGSRLFDLDGNEYIDFMLNMGAQLVGHAHPAVVKAIG
                     EQAERGTLFCMPHLWEQEVARQLASRFGIPQWRYVNSGTEATMTAIRIARGYTGKKYI
                     VKFEGHYHGHSDQTLVTTNALLRQLGREDRGVKVPASQGIPEETYALTLMAVFNNLDS
                     VRDLFARHGGEIACVIAEPIMMDCGCIEPEPGFFEAVRDLCHANGALLVFDEVKTGCK
                     VAPGGASQLYGVTPDIVCIAKALAGGLPMGAVGASEEIMQVVGDGSVLHVGTFGANPL
                     VLYVAHVVLRDLLTEEAYRHTFALNRELADGYREIVRRHGLQAQINAVGPCGMITFTD
                     RPLRSYREFMTADEDRFRLFWFGMMNQGILPAHHFGGDVWTVSIAHTRDDVARNLEAF
                     DRLAPRLR"
BASE COUNT          392 a          867 c          879 g          382 t
ORIGIN      
        1 cgccgacgtg cgcccgtggc gcgagggggg gaccggcggg gacgccagcc ggccgctgca
       61 gccggaggaa ttcccgcacc gcctcgaagg ggggacgccg aacgtcttcg gcatcgccgg
      121 gctgcgcgcc gggctccggt gggtgctcga gcgcggccct gaggcgatcc tggcgcacga
      181 gcgccggctc ctgcgcggat tcctcgccgc cctgaagcgc gccgacgcgt tcgcgtggta
      241 cggcgccgat cgcgccctgc tcgatcgggg gggcgagggg cgggtgggcc tggtcgcctt
      301 caacgcgcgc ggcacgcccc cgcaggcgat cgccgcggcg ctcgacgagc gcttcgggat
      361 cgccgtccgg cccggtctcc actgcgcccc gtacgcccac cgccacctcg gcaccttccc
      421 ctacggggcc gtgcgcatca gcgtgggcgc cctgacccgc cccgaggaac tcgaggcggc
      481 cgccgcggcg ctcgaccagc tcgccggctg acgaccctcc gggcgccgga gcgtccggct
      541 tgacagtccc ccccgcccgg gcccaggatg gttgctgtct ccgggtttcg tgatgcgcaa
      601 cacacccatc agcgttctgg cgcgggccgc gacgttcggc gtgccgcatt ccaacgagtg
      661 cccgcacctg accacctacc cccgcaagat caagcacctg cacgacgaca cctagcctcc
      721 ccgcgaggcg ccggttcccc gtcaactcgg ggcgtcgctt tcgtgtcgac gcccgcagtg
      781 tcttcaacgc gcagggaaag gagtcgtccg atgccagccg tccaggaaga cccggcgaca
      841 ccatccgccg ggattcgcga ggagatggag cgccaggtgg ccgcctggag cgcgaagacc
      901 gggaagtcgc gggaggcgtg ggagcgggcg cgccggaacg tccccggggg agtctccggg
      961 aacttccggc tgatgaatcc ctacccgatg ttcgcggcgc gggcgaaggg cagccgtctc
     1021 ttcgacctcg acggcaacga gtacatcgac ttcatgctca acatgggggc ccagctcgtc
     1081 gggcacgcgc acccggcagt cgtcaaggcg atcggcgagc aggcggagcg cggcaccctc
     1141 ttctgcatgc cgcacctgtg ggagcaggag gtggcgcgcc agctcgcctc ccgcttcggc
     1201 atcccgcagt ggcgctacgt caactccggg accgaggcga ccatgacggc gatccggatc
     1261 gcccgcggct acaccggcaa gaaatatatc gtgaagttcg aagggcacta ccacggtcac
     1321 agcgaccaga cgctggtgac gacgaacgcg ctcctgcgcc agctcgggcg ggaggatcgc
     1381 ggcgtgaagg tgccggcgtc gcaggggatc ccggaggaga cctacgccct cacgctgatg
     1441 gcggtcttca acaacctcga ctcggtgcgg gacctgttcg cgcgccacgg cggggagatc
     1501 gcctgcgtca tcgccgagcc gatcatgatg gactgcggct gcatcgagcc ggagcccggg
     1561 ttcttcgagg cggtgcgcga tctctgccac gcgaacggcg cgctgctcgt cttcgacgag
     1621 gtgaagaccg gctgcaaggt ggcccccggc ggggcgagcc agctgtacgg cgtgacgccg
     1681 gacatcgtct gcatcgccaa ggcgctcgcc ggcggcctgc cgatgggggc ggtcggcgcg
     1741 tcggaggaaa tcatgcaggt ggtcggggac ggcagcgtcc tgcacgtcgg caccttcggc
     1801 gccaatccgc tggtgctgta cgtcgcccac gtggtgctgc gcgacctgct caccgaggag
     1861 gcgtaccgtc acaccttcgc cctcaaccgc gagctggcgg acgggtaccg ggagatcgtg
     1921 cggaggcacg ggctccaggc gcagatcaac gcggtggggc cgtgcggcat gatcaccttc
     1981 acggaccggc cgctgcggtc ctaccgggag ttcatgaccg ccgacgagga ccgcttccgg
     2041 ctcttctggt tcgggatgat gaaccagggg atcctgccgg cgcaccactt cggcggcgac
     2101 gtctggacgg tgtcgatcgc gcacacgcgc gacgacgtgg cgagaaacct cgaagcgttc
     2161 gacaggctcg caccccggct gcgataagtg tttgctcgag gtactcgtca gtgggaggct
     2221 tatgcgtcac cgacacctct gaaattgggt cagatgtgcg gtcccggctc tctcgcggag
     2281 cggagaggtt cagatctctt tcgggagata ccctctatct gagtggatta catccagcga
     2341 gacttggaga cttggcaagc ggagagccag tgccagtgga agtcaggtta acccttcgcc
     2401 ccccgggttc gagaagagag ggggagtggc tccccatgtg tcattctcct cgaacctccg
     2461 gctatcaact gcgacggcta ggacacgatc cggtcacctt ccatcacttt cacagtgcct
//
LOCUS       DASRIQ010000055         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_539578, whole genome shotgun sequence.
ACCESSION   DASRIQ010000055 DASRIQ010000000
VERSION     DASRIQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539578"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFD06_01405"
     CDS             <1..826
                     /locus_tag="VFD06_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN02221.1"
                     /translation="HPDVDKIAFTGSMEVGKLIMRSAADTLKKVSLELGGKSPNIFFA
                     DADFEAAVDGALFGVFINQGEVCSAGSRVLVQRPIYKKLVDAMVEKAKRIPVGPGIDP
                     ANRMGPLVSQEQYDRVLGYLAKGKSEGKLAVGGGRPETLAAGAEKGFFVSPTIFYDVD
                     NRATIAREEIFGPVMAVIPFDDEAEALRIANDTHYGLAAAVWTRDLFKAMRTVRRLRA
                     GVVWVNHMQPTPVEAPWGGFKQSGFGRELGPWGLDEYLEVKQVYVNLDETPIGWYR"
     gene            complement(789..2744)
                     /locus_tag="VFD06_01410"
     CDS             complement(789..2744)
                     /locus_tag="VFD06_01410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN02222.1"
                     /translation="MQRDDLRLFLGVFGTALVARFAAFRLTFDPSLVFEKYLLLAHDL
                     LAHGVREPWAFSPVYTLCVAAVLGLGGAARAIVVSQVFLGAIACVLVAALALRLFGRR
                     EAVIAGIAAALYGPFLLASVELESDGVGLVLGLGAALAAARAAERPSYTAWGIAGLLV
                     GLRSIHRPDALLLVLLLPVAQLVFGDRARARTAWALVPFALLPIAPIAWQNFRVSGEF
                     IPVTSSGGWVFYTSHNWQERGLSYFPPPLAWQWMQGPAKDPLDRLDDRVSRRLASLSL
                     GTEIDAAEASRFWRHEGIVSMGERGFAAQAALEARRLLYMLHGYEAHDNLPLLVKQER
                     LGAWGRGMGVLAPFALLGLALACSRPDFRRRNCSWLPPFLLLPVVSMGVFYVGARLRL
                     ELAALLMPFAAAAVLALWNAWRAGRASEALRGAAIVVACALLLNLPDREIERQRRLRF
                     IQVRTFLGERTGDEAELRRAIREAATPAEAEPAWRLLAGFLRSRGEQERAQAAERTAA
                     GFLDDRTLEELRDRPGDPDTLWAVARHHLVRGEPDRAAAILRRAVALAPDDPDLAFAL
                     ATAAFDAGGTPAERILAAVQAAFRKGLRFSTNAAAGYLLLGRCALMTGQAELARPAFE
                     AALRYDPDNEPARRLLSDTTRSASRRD"
BASE COUNT          460 a          974 c         1003 g          313 t
ORIGIN      
        1 ccaccctgac gtggacaaga tcgccttcac cgggagcatg gaggtcggca agctcatcat
       61 gcgcagcgcc gccgacacgc tgaagaaggt gagcctcgag ctcggcggca agtcccccaa
      121 catcttcttc gccgacgccg acttcgaggc ggcggtggac ggggcgctct tcggcgtctt
      181 catcaaccag ggggaggtct gctccgccgg cagccgcgtg ctggtgcagc ggccgatcta
      241 caagaagctc gtggacgcga tggtggagaa ggcgaagcgg attcccgtcg gaccggggat
      301 cgaccccgcc aaccgcatgg ggccgctcgt ctcgcaggag cagtacgacc gcgtcctcgg
      361 ctacctcgcc aaggggaaga gcgaggggaa gctcgccgtc ggcggcggcc gccccgagac
      421 actggccgcc ggagccgaga agggattctt cgtgtccccg acgatcttct acgacgtgga
      481 caaccgggcg acgatcgccc gcgaggagat tttcggtccg gtgatggcgg tgatcccatt
      541 cgacgacgag gcggaggcgc tgcgcatcgc caacgacacc cactacgggc tggccgcggc
      601 ggtctggacc cgcgacctgt tcaaggcgat gcggaccgtc cgccggctgc gcgccggggt
      661 ggtgtgggtg aaccacatgc agccgactcc cgtggaagcg ccgtggggcg gcttcaagca
      721 gtccgggttc ggccgcgagc tgggaccgtg gggcctcgac gagtacctcg aggtcaagca
      781 ggtctacgtc aatctcgacg agacgccgat cgggtggtat cgctgagcag gcgccgggcc
      841 ggctcattgt ccgggtcgta gcgcagcgcg gcctcgaagg cgggacgcgc cagctccgcc
      901 tgcccggtca tcagggcgca gcgccccagc aggagatagc cggcggccgc gttggtcgag
      961 aagcgcagcc ccttccggaa ggccgcctgc acggcggcga ggatccgttc ggccggcgtt
     1021 ccaccggcgt cgaacgcggc ggtggcgagg gcgaacgcaa gatcggggtc gtccggcgcc
     1081 agcgccacgg cgcgcctcag aatcgccgcc gcgcgatccg gctcgccgcg cacgaggtga
     1141 tggcgcgcca ccgcccagag agtgtccgga tcgccggggc gatcccgcag ctcttcgagg
     1201 gtccgatcgt cgagaaaccc cgccgcggtc ctttcggccg cctgcgcccg ctcctgttcg
     1261 ccccgcgatc tcagaaaacc cgccagcagg cgccaggccg gttccgcctc cgcgggtgtg
     1321 gcggcctccc ggatcgcccg gcgcagctcc gcttcgtcgc cggtccgctc cccgaggaaa
     1381 gtgcgcacct ggatgaagcg caggcggcgc tgccgctcga tctcccgatc cggaaggttg
     1441 aggagcaggg cgcaggcgac gacgatcgcg gcgccgcgaa gcgcctcgga ggcgcggccg
     1501 gcgcgccacg cgttccagag ggccaggacc gccgcggcgg cgaacggcat caagagcgcc
     1561 gccagctcga gacggagccg cgcgccgacg tagaacaccc ccatcgagac aaccggcagc
     1621 aggaggaacg gcggaagcca ggaacagttc cggcgtcgga agtcggggcg gctgcacgcc
     1681 agcgccagcc ccaggagggc gaacggcgcg aggaccccca tgccgcgtcc ccaggccccc
     1741 aggcgctcct gcttgacgag cagcggcaga ttgtcgtgcg cctcgtagcc gtgcagcatg
     1801 tagagaagcc gccgcgcctc gagcgccgcc tgcgccgcga agccgcgctc ccccatgctg
     1861 acgatcccct cgtgacgcca gaagcgggac gcctccgccg cgtcgatctc ggtccccagc
     1921 gacagggagg ccagccggcg cgacacccgg tcgtcgagcc ggtccagcgg gtctttggcc
     1981 ggcccctgca tccactgcca ggcgagcggc ggcgggaagt acgacaggcc gcgctcctgc
     2041 cagttgtggc tggtgtagaa gacccagcct cccgacgagg tcaccgggat gaattccccc
     2101 gagacgcgga agttctgcca ggcgatcggc gcgatcggga ggagcgcgaa cggcaccagc
     2161 gcccaggccg tacgcgcgcg ggcccggtcg ccgaagacga gttgcgccac cggcaggaga
     2221 aggacgagca gcagcgcgtc cggccggtgg atcgatcgca gcccgacgag caggccggcg
     2281 atcccccagg cggtgtacga cggccgctcg gcggcccgcg ccgccgcgag cgccgcgccg
     2341 agtccgagga cgagccccac tccgtcggac tcgagctcga cggaagcgag caggaacggc
     2401 ccgtagagcg cggcggcgat cccggcgatc accgcctcgc ggcggccgaa cagccgcagc
     2461 gccaacgccg ccaccagcac gcaggcgatc gccccgagga acacctgcga cacgacgatg
     2521 gcgcgcgccg cgccgccgag cccgagcacc gccgcgacgc acagcgtgta gacgggagag
     2581 aaggcccagg gctcgcgcac gccgtgggcc aggagatcgt gcgccaggag aaggtacttc
     2641 tcgaagacga gcgacgggtc gaaggtcagg cggaacgcgg cgaaacgcgc gacgagcgcg
     2701 gtgccgaaga cgccgaggaa gagccggagg tcgtcgcgtt gcatccgtca
//
LOCUS       DASRIQ010000056        15300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2887712, whole genome shotgun sequence.
ACCESSION   DASRIQ010000056 DASRIQ010000000
VERSION     DASRIQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 15300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15300
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2887712"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFD06_01415"
     CDS             complement(<1..556)
                     /locus_tag="VFD06_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02223.1"
                     /translation="MTFTHDVLHRHRILGATSGLSFTYHDGGEVATILNASGQGRTFG
                     YDAAGRLGSVSDDVSGWTAAQTYNPQGERVKRVESSGGVTTTTYFVGKLYDAQGTSHN
                     DYIYLGGQRIVEIRATAETVGVFYLLNDHLGSVNVVTDHTGGFVQRLAWNPYGELRQA
                     EGQSFSAAFPFAGARRDPGLGLDDF"
     gene            complement(565..861)
                     /locus_tag="VFD06_01420"
     CDS             complement(565..861)
                     /locus_tag="VFD06_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm27 family immunity protein"
                     /protein_id="HZN02224.1"
                     /translation="MTAALIASHETEIRGKWNMIGGKVEADDQCLRIEDLVTQHLREV
                     GRDATGWDRLFIDPQDGRYWELTYPESELHGGGPPRLRNLQELEARAKYGQFGS"
     gene            complement(858..1058)
                     /locus_tag="VFD06_01425"
     CDS             complement(858..1058)
                     /locus_tag="VFD06_01425"
                     /inference="COORDINATES: protein motif:HMM:NF026858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 10 domain-containing
                     protein"
                     /protein_id="HZN02225.1"
                     /translation="MRHEYREGHSGSFTVIEFATPSMGLASPIFRKSPGFVGGGLTSG
                     GAREFVLPNGPIPADALIRVSP"
     gene            complement(1039..2094)
                     /locus_tag="VFD06_01430"
     CDS             complement(1039..2094)
                     /locus_tag="VFD06_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZN02226.1"
                     /translation="MASARWLHRRRATRMSIARRLAVSASALSHWLRRWRENHLTVRP
                     RGRPAEEVDRETREDVLAVFALMGPHATLTALRDLFPQVARGALENLLERCRRVYRRG
                     AFWHLRTLRWTRPGSVWAMDFTEPPAWIDGVYRYLLVIRDLASGRQLLTQPCTNPSAQ
                     LTLDALRTSFLWCGKPLVLKMDNGSAFIAAEVKTFLAGLGVFPLYSPPCTPSYNGSVE
                     AGIGALEVRAFFESARHDRPGRWTSDDVAAARLQANATIRRFGPGTPTPDEMWNGRTP
                     ISTLESHVFRLAYDRERLRACAERDDAPCDRESHRFRASIDRVALSRALIVRGLLLLR
                     SRRLTPPITLRSAARIS"
     gene            complement(2208..2633)
                     /locus_tag="VFD06_01435"
     CDS             complement(2208..2633)
                     /locus_tag="VFD06_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN02227.1"
                     /translation="MSRGRPPDGVGHVDRLEGPDELKRRLKVLLATLDGSKSVPEACA
                     ELQVSESRLHELRREALEGALRALQPRPSGRPSKPEAATPRERELEAHIATLEEDLQA
                     AYVRTELALAMPHLFKGKKNLRIRKSRPLPGASGGGDGT"
     gene            complement(2646..3104)
                     /locus_tag="VFD06_01440"
     CDS             complement(2646..3104)
                     /locus_tag="VFD06_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02228.1"
                     /translation="MSGPARATRAAKPLEGDETAKKTAAMILEVLAGLRSTTEAAQAL
                     EISSMRYYVLERRAMEGMVQALQPRPKGKQKRPENTLEGLRRDKARLEREVGRLQALV
                     RAAQRTMKLPPLPSREKKKGDGRRGRRPQVRAERTIALLQAPATETTPVP"
     gene            complement(3101..4513)
                     /locus_tag="VFD06_01445"
     CDS             complement(3101..4513)
                     /locus_tag="VFD06_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZN02229.1"
                     /translation="MSVHDERESGEVEVRLKEPGETKAEAAGETSVPRLFSELGTGIS
                     TSPLQPASDTEEPAMEEEASEPERPPRLLTGRRKGSVYRRESEMQPQVAGQALSGEQR
                     LLILDAWRRSELTAGEFGKIAGVTAHTLYAWRRRFEALGPAGLDEKKRGAPSGSRVAE
                     ATKRAILMMKEKHEEWGCERIHDMLMRSAGFMASAGAVAKVLREAGYESEARKSEAHP
                     DKPREFERAKPNQLWQSDLFTFLLKREGRRLYLVGFMDDHSRFLVGCGIFASSSGAVV
                     REVFEAAIANFGAPEEVLTDQGPQYHTWRGTSEFGKLCAKRGIRQIVARARHPQTLGK
                     IERFWGTLWRECVEQAVFQSLDDARRRVGLFIDYYNFQRTHQGLDGLVPADRYFQAAP
                     QVLETLKKRVEANALDIAQHGTPRQSVYLTGRVGEAGITLHGEGGKLVLQTSEGGRQE
                     VDLTAPGRRAPLSDKEGGEA"
     gene            5196..7385
                     /locus_tag="VFD06_01450"
                     /pseudo
     CDS             5196..7385
                     /locus_tag="VFD06_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
     gene            complement(7525..9729)
                     /locus_tag="VFD06_01455"
     CDS             complement(7525..9729)
                     /locus_tag="VFD06_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN02230.1"
                     /translation="MSAAARRARAVWMLGYLALFAVAALFGALAWRLTPLEGSTLKRV
                     RRGAGHGAIPEEPSLVLISVDGLRIGRLGSYGDAPLSPTPHLDRLAAEGFRFEQATTP
                     VPETLPAHLSMMTGASPVGRPWLLRPGAALPPDAATLAEVLHGAGYQTAAFVGAAAVG
                     RATGLARGFDRFDEPRVGDRPATIRWLAERPAADVVDAARAWLDDHFRSRFYLWVQLS
                     DPTPPHPADPPRTLRHRDPYDAELERTDAEIGRLLQRLASLGVAGTTIVVVAGSHGEG
                     LGEHAERGSGFGLYDATLRVPLLLRVPGLVVRDRSIPEPVRLFDLAPTLLDLLRLAAP
                     RPMEGTSLVPLLDPEGRMPPLPARAEAVAFPNYLGDRSRVAIRGGGFKLIHGDTLELY
                     DLKRDPGEARNLASVQQDRVVEIGRQWPEAFGSGTGTFDADALLSGPPIPSPPGASAL
                     VTDALTALRAREGPKAWRALEDLRSLRQRSGERSTPPGLLAISGGTLRLLGETRQALD
                     LYEAALRQLTTPRAVAAADPAATALTPGAPPGAGDPAKDSAHPARAVQAAAAPGGAPG
                     SEAGAAPGHEDPLRALVLAEIGACRRVLGDRDGAIEAYRAAAAARLDDVDGRLALAET
                     LLEAGAVDEAIDELRAALSRAPGEPELLAGLGRAQVAAGQAEAGMRALQDAIRAAPWL
                     HRPWLDLGGAYEALGRVDEARRAYQDVQARTDPDDPLHREAAERERALRSRS"
     gene            complement(9726..10952)
                     /gene="glp"
                     /locus_tag="VFD06_01460"
     CDS             complement(9726..10952)
                     /gene="glp"
                     /locus_tag="VFD06_01460"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZN02231.1"
                     /translation="MPDMIAVERALDLVLEAARPLPPIVADLADLPGRILLEEIRADR
                     DVPSWDKSLMDGFAVAAADLGRGKEVAIAATIAAGADPGALAALPPGQAARIMTGAPL
                     PRGADAVIPVERSRAGSQPDRTALQGPVRPGENVARRGDDVREGAVLLEPGDFLGAAE
                     IAVLAACGRIRARAGGRPRVAVLATGDELVPPGEEPGPGRLRNSNGPMLEALARRAGA
                     EVRDLGIARDDEEDLRRAIASGLDADVLLLSGGVSMGLYDLVGGALRSAGAEVLFERV
                     AIRPGKPFTFARRGGTLVFGCPGNPVSSYVIFQVFARPALRRLASHPTPVWRMQPGRL
                     ASPIRQKPGRAGYVQARAVFRDGRLDVEPVPTSGSADLVSCARGNALAIVPAEAASLP
                     AGSDVAVILLDDHGDR"
     gene            complement(11015..11890)
                     /gene="prmC"
                     /locus_tag="VFD06_01465"
     CDS             complement(11015..11890)
                     /gene="prmC"
                     /locus_tag="VFD06_01465"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011908152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZN02232.1"
                     /translation="MTPAVAATLVREASALFAAAGIDAPERDAEVLLAHVLGIDRGAL
                     HARPDRPMEGDAVRRYRELVRRRAAREPIQHLTGQQEFWSLPFRVTPDVLIPRPETEG
                     ILEALLERALPVEPGRPRLLDLGTGSGCLAIAAARALPGARVTAVDDSEAALEVARSN
                     AEALGVSDRVAFALGDLYAALPSGTSDGPFDAILSNPPYVAVADLDGLAPEVRDHEPR
                     RALTPGADALAVHRRILQGAPRVLAAGGWLLVEIGAGQEAAVRALYATQEAIRLEEIR
                     PDLAGLPRVVVARAA"
     gene            complement(11887..12963)
                     /gene="prfA"
                     /locus_tag="VFD06_01470"
     CDS             complement(11887..12963)
                     /gene="prfA"
                     /locus_tag="VFD06_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZN02233.1"
                     /translation="MELQERLRTIAAKFEAFAAQMADPGIAADPDRYRQIAKAYSELE
                     RVVERYREYEKIEGEIREAQSLADDPDLRGMAREDLALLEPKRDALLAELRELLLPRD
                     PNDEKNVILEIRAGTGGEEASLFASELYRMYSRYAERRGWTVEVLTLSSTGLGGLKEV
                     IALISGKQVYSRLKHESGVHRVQRVPATESAGRIHTSTATVAVLPEADDVQIQINDKD
                     LRIDTFCATGPGGQGVNTTYSAIRITHLPTGLVVQCQDERSQHKNKARAMKVLRSRML
                     EVEQQKQRDAIAQDRRSQVGTGERSEKIRTYNFPQNRVTDHRVNVTLHRLPDLLDGAL
                     DPLVEPILAHYQAERLKEEVGAGA"
     gene            complement(12983..13309)
                     /gene="rpmE"
                     /locus_tag="VFD06_01475"
     CDS             complement(12983..13309)
                     /gene="rpmE"
                     /locus_tag="VFD06_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZN02234.1"
                     /translation="MKSGIHPKYHVVTVSCACGETFQTRSTNPELRLEICSKCHPFFT
                     GKQKLIDSAGRVERFEKRYKATQEATPPAAAAAPAASVSRAARRIGATRSKPEKKPRK
                     GTTAGV"
     gene            complement(13463..14776)
                     /gene="rho"
                     /locus_tag="VFD06_01480"
     CDS             complement(13463..14776)
                     /gene="rho"
                     /locus_tag="VFD06_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZN02235.1"
                     /translation="MVDDEVERDDVPRAELSGEKLDIRVLKEKSVSELTGIAKALDVA
                     NATALRKQELIFEILRAQTEKSGLIFAEGVLETLPDGFGFLRAPEYNYLPGPDDIYVS
                     PSQIRKFDLHTGDTISGQVRPPKEGERYFALIKVEAVNFEHPEQARNKIFFDNLTPLY
                     PNTRIKLETTRENVSSRVLDILCPIGKGQRGLIVAAPRTGKTMLLQAIANSITQNHPE
                     VVLIVLLIDERPEEVTDMQRSVNGEVISSTFDEPATRHVQVAEMVIEKAKRLVEHRKD
                     VVILLDSVTRLARAYNTIVPPSGKVLSGGVDANALQRPKRFFGAARNIEEGGSLTIMA
                     TALIETGSRMDDVIFEEFKGTGNMEIHLDRKLADRRIYPSIEITRSGTRKEELLLSKK
                     ELDRSWVLRKVLNSLSPVEAMELLIERMQKSKSNEEFLDSMSSMG"
     gene            complement(14907..>15300)
                     /locus_tag="VFD06_01485"
     CDS             complement(14907..>15300)
                     /locus_tag="VFD06_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02236.1"
                     /translation="LVEAYLEGAPGPDLSILLVDARHPPTDLDLELWDWLSAAHRPAR
                     IALTKIDKLPRGRWAAVTRAAAAAFQSEPEPPVPVSAVTGDGIPALWRILDAACERGR
                     NASPGAEPLVPPHPHGEGHHRERQRHRA"
BASE COUNT         2069 a         5661 c         5323 g         2247 t
ORIGIN      
        1 cgaaatcgtc caggcccaga ccgggatcgc gcctggcgcc ggcgaacgga aacgccgccg
       61 agaacgactg cccttccgcc tgcctcaact ctccgtacgg gttccaggcg agtctctgaa
      121 cgaagccgcc ggtgtgatcg gtgacgacgt tgacgctccc cagatggtca ttgaggagat
      181 agaacacccc gaccgtctcg gccgtcgcgc gaatctcgac gatgcgttga ccaccaagat
      241 agatgtagtc gttgtgcgag gtcccctgtg cgtcgtaaag cttgccgacg aaataggtgg
      301 tcgtcgtgac accgccgctg gactcgaccc gcttgacgcg ctccccttgt gggttgtatg
      361 tctgcgccgc cgtccatccg gacacatcgt cgctgacgct gccaagccgt cccgccgcgt
      421 cgtagccgaa cgtccgcccc tgtccggaag cattgaggat ggtcgcaact tcgccgccgt
      481 catggtaggt gaacgaaagg ccgctcgtgg cgcccaggat gcgatgccgg tggagcacgt
      541 cgtgtgtgaa cgtcacccct tccctcatga tccgaactgg ccgtacttag ctcgcgcttc
      601 caattcctgc aggttgcgca aacgcggcgg cccgccaccg tgcagttcgc tttccgggta
      661 ggtcagctcc caatatcggc cgtcttgcgg gtcgatgaac agccgatccc aaccggttgc
      721 atctcgacct acctcccgca aatgttgggt gaccagatcc tcaatgcgta ggcattggtc
      781 atcggcctca actttacctc cgatcatgtt ccactttccc cggatctcgg tttcgtggct
      841 tgcgatcagg gctgcggtca tgggcttacc ctgatgagag catccgctgg tattgggcca
      901 ttaggcagaa cgaattcacg ggcgccacca ctcgtgagtc ccccaccgac aaatccaggg
      961 ctcttccgga agataggcga ggcaaggccc atggacggcg tcgcgaattc gatcacggtg
     1021 aatgacccgc tgtgcccctc acgatattcg tgccgcagat cgcagggtaa tcggtggagt
     1081 aagtcgcctg cttcgtaaga gcagaagacc gcgtacgatg agagcgcggc tgagggcgac
     1141 gcggtcgatg gacgccctga accggtgtga ttcgcgatcg catggtgcat cgtctcgctc
     1201 ggcgcacgcg cgtagtcgtt cgcgatcata cgccaggcgg aacacgtgtg actcgagagt
     1261 gctgatcgga gtgcgcccgt tccacatctc gtcgggcgtc ggcgttccgg gaccgaagcg
     1321 acggatggtc gcgttggcct gcagccgcgc ggcggcgacg tcgtcgctcg tccagcggcc
     1381 cggccgatcg tgccgggccg actcgaagaa ggcgcggacc tcgagcgcgc cgatgcccgc
     1441 ctcgacgctg ccgttgtacg acggcgtgca gggcggcgag tacagcggga agacgccgag
     1501 cccggcgagg aacgtcttga cctctgcggc gatgaacgcg gacccgttgt ccatcttgag
     1561 caccagcggc ttgccgcacc agaggaacga cgtgcgcagg gcatcgaggg tgagttgcgc
     1621 cgatgggttc gtgcacggct gggtcaacag ctgtcgtccg ctcgccaggt cccggatcac
     1681 cagaaggtag cgatagacgc cgtcgatcca ggccggcggt tcggtgaagt ccatcgccca
     1741 gacgcttccg ggccgggtcc agcgcagcgt ccgcaggtgc cagaacgcgc cgcggcggta
     1801 gacccgccgg cagcgctcca agaggttttc cagagcgccg cgcgccacct gcgggaacag
     1861 atcgcgcaac gccgtcaagg tcgcgtgcgg tcccatcagc gcgaacaccg ccaggacgtc
     1921 ctcgcgggtc tcccggtcca cctcttcagc cggacggccg cgaggcctga cggtcagatg
     1981 gttctccctc cagcgacgca gccagtgcga caacgccgaa gccgacacgg ccagccgccg
     2041 ggcgatcgac atccgggtcg cacggcgccg gtgcagccaa cgcgcggacg ccaccgccga
     2101 gacccgcgcc gtgcgctcgg catcccgcac gcgacgctga cgcttccacc cgcgccggta
     2161 ccgaaccgga cgcgcgtgtt gcgcctcgat gatgcggcgc aaccgcttca cgtgccgtct
     2221 ccaccgccgc tcgcgccggg taacggccgg ctcttccgga tgcggaggtt ttttttaccc
     2281 ttgaacaggt gcggcatcgc caaagccagc tccgtccgca cataggccgc ctgcaggtcc
     2341 tcctccagcg tcgcgatgtg cgcctcgagc tcgcgctccc gcggggttgc cgcttccggc
     2401 ttcgaaggcc gccccgacgg ccgcggctgc aacgcccgca aggccccttc gagggcctcg
     2461 cgccgcagct cgtgcagccg cgactcgctc acctgcagct cagcacacgc ctccggcacg
     2521 ctcttgctcc cgtcgagcgt ggccagcagc accttcaacc gccgcttcaa ctcgtccgga
     2581 ccttccaggc gatccacgtg gcccacaccg tccggtggtc gtcctcggct cacggagttc
     2641 ctcctctaag gaaccggtgt cgtctcggtc gccggcgcct gcaacaacgc gatcgtcctc
     2701 tccgccctga cttgaggtcg gcgcccccgc cgcccgtccc ccttcttctt ctctcgcgag
     2761 ggaagcggcg gcagcttcat cgtccgctgc gccgcccgca ccagcgcctg cagccgtccg
     2821 acctcccgct ccaggcgcgc cttgtcgcgc cgcaacccct cgagcgtgtt ctccgggcgc
     2881 ttttgctttc ccttcggccg cggctgcagc gcctgcacca tcccttccat cgcccgccgc
     2941 tccaacacgt agtagcgcat cgacgagatc tccagagcct gcgccgcctc cgtcgtgctc
     3001 cgcagccccg ccagcacctc caggatcatc gccgccgtct tcttcgccgt ctcgtcccct
     3061 tcaagcggct tcgcggcacg cgtcgctctc gccgggccgc tcatgcctca cctccttctt
     3121 tatcagagag cggcgcgcgc cgtcccggtg cggtcagatc cacctcctgc cgcccgcctt
     3181 cgctcgtctg cagcaccagc ttccccccct cgccgtgcag cgtgattccc gcttcaccga
     3241 cgcgcccggt cagatacacg ctctgtcgtg gcgtcccgtg ctgcgcgatg tccagcgcgt
     3301 tcgcctccac ccgcttcttc aacgtctcca acacctgcgg ggccgcctgg aagtaccggt
     3361 ccgccggcac caggccgtcc aacccctgat gcgtgcgttg gaagttgtag tagtcgatga
     3421 acagcccgac ccgtcgccgt gcgtcgtcca ggctctggaa caccgcctgc tccacgcact
     3481 cccgccacaa cgtcccccag aagcgctcga tcttccccag cgtctgcgga tgccgcgccc
     3541 gcgccacgat ctgcctgatc ccccgcttcg cgcacagctt cccgaactcg ctcgtcccgc
     3601 gccacgtgtg gtactgcggc ccctggtccg tcaacacctc ctccggcgcc ccaaagttgg
     3661 cgatcgccgc ctcgaacact tcccgcacca ccgcccccga cgacgacgcg aagatcccgc
     3721 accccaccag gaaccggctg tggtcgtcca tgaaccccac caggtacaac cgccgtccct
     3781 cacgcttcaa caggaacgtg aacagatccg actgccacaa ctggttcggc ttcgcccgct
     3841 cgaactcccg cggcttgtcc ggatgggcct cgctcttgcg cgcctcgctc tcgtaccccg
     3901 cctcgcgcag caccttggcc acagcccccg ctgacgccat gaaccccgcc gagcgcatca
     3961 gcatgtcgtg gatgcgctcg cacccccact cctcgtgctt ctccttcatc atcaggatcg
     4021 cccgcttggt cgcctccgcc acccggcttc ctgacggcgc cccgcgcttc ttctcgtcca
     4081 accccgccgg ccccagcgct tcgaaccgcc gccgccacgc gtacagcgtg tgcgccgtca
     4141 ccccggcgat cttcccgaac tcgcccgccg tcagctcgct ccgccgccac gcgtccaaga
     4201 tcagcaaccg ctgctccccg ctcagcgcct gccccgccac ctgcggctgc atctccgact
     4261 cccgccggta caccgacccc ttccgccgac ccgtcaacag ccgcggcgga cgctccggct
     4321 cgctcgcctc ttcctccata gccggctctt ccgtgtcgct cgccggctgc aacgggctcg
     4381 tcgagatccc cgtccccagc tccgagaaca accgcggcac cgaagtctcg cccgccgctt
     4441 cggcctttgt ctcgcccggc tccttcagac gcacttccac ctcgcccgac tcacgttcgt
     4501 cgtgcaccga catccccgcc tccccgcggc ctacgccgcg actggaagca ggctcttact
     4561 ccaccgattt atcgattgaa ctgaggacga atttcgggaa gggagacagc gcctcgcatc
     4621 ggctcgcggt cgtccgacgt gaggatgaga gtacgtgact tatactcgcg ggcgtgtctc
     4681 gtcaggcgcg cacccgcgcg ctcgtcgccg gagccctgct cgccgcgctg atcctgctgt
     4741 tcgcgtcact ggtgcgcgtc ccggaagggc aggtcgcgct cgtctccggt cgcgcgcgat
     4801 cctccggatg gcacttgaag gccccgttcg cgcggacgcc ctctgtgccg gccgcggggc
     4861 ggctcgaggg gatcgaggtc gcggtcacga cgccggaggg ggcgacgctc agggcgcgtc
     4921 tttccttcga ctaccggatc gagccgggac gcttcgcgcc gccggatcgg gacagcgccg
     4981 cggccggcct ccctgcgatc gcccggcgcc tcgtggggga ggcgctggcg acgggaggcg
     5041 cggggatgga gacgccggcc ggggacgcgc cgctgtcgcc ggatcgcctg gatcgcatcg
     5101 cgcggcacct cgcagagcgc ggcatcgtca tctcctcggc gaccgggacg atcggcgcgc
     5161 ccgagggctc agcggccgcc ggtccgcgcg gccccatgcc gccgcgggat ccgaccggga
     5221 tccgggtgct gctcatcggc ctcgacggcg ccgactggcg caccatcgat ccgctgatcc
     5281 gggcgggccg gatgccgcgg ctgcagcggc tcgtcgccga aggcgcgcgc gcccccctgc
     5341 gctcctacga tccgatgatc tctccgctcc tgtggacgac catcgtcacc ggggtcggcc
     5401 ccgacgtcca cggcgtcgcc gacttccagg cgatcgagca gccgagcagc cggcgcattc
     5461 cgatcaccag ccgcttccgc aaggtcaagg cgctgtggaa catcctcgac gacgccggga
     5521 tgacgagcgc cttcgtcgcc tggtgggcct cgtatccggc agagcgggtg cgcggcttcc
     5581 aggtctccaa tctcctcgcc ttcgagtcgc tgcggccccg gccgcgcgac aaaccctggc
     5641 cgccggggat cgtctggccg ggcgactacc tggcgcagaa gggcgctctc ttgaagaccg
     5701 ccgccgatct gcccgaggcg gagatccgcc ccatcctgca cctcgacgcg gccgccctcg
     5761 cgaaggcgcg cggagaggtc ctgaaggagc ccagtgacgc ggtgccggcg gcgcgccggg
     5821 cggtgcagga cccggcggcc cttgctctct cgatcctgac cggcacgggc aactacgcgc
     5881 gcgtggcggc cgacctcgcc gcccgccgcg ccgatctcac cggcgtctat ttcgagggga
     5941 tcgacatgat ggggcaccgc ttccagcact gcatgccccc gcgcatgccg atctgcccgg
     6001 aggaggacta cgcgcgctac cgggacgcgg tcacaggctt ctacgcgtac caggaccggc
     6061 tgctgggcga agtcctcgac gccgccggtc cggggacgac cgtgctcgtc ctctccgacc
     6121 acggtttccg ctccggcgcc gggcgtccgg agggggtgct cccctacacg accgagcagc
     6181 cggtggagtg gcacgacaag gaggggatct tcgtcctgag cgggcctgcc gcgcggcgcg
     6241 gcgcccggct ctccgggatt ccgacgctgt tcgacatcgc cccgacgatc ctcgccctcc
     6301 tcggtctccc ggtgtccgac gcgatgccgg gccgggtcgt cgtcccggcg ctccagccgg
     6361 agttcctggc ggcgcatccg ccgcgccgca tcccgtcgta cgaaacggtc ggatcgccgc
     6421 gggagcgcga gccgtccgcg ggggatccgg cggcgcgcga ggcggaggcg gagctcctcg
     6481 cgagcctccg ttcgctcggt tacatcggcg gggacgaggg gacgcaggag gcgccggcgg
     6541 cgggcaccgg cgctccgggc ggcgccccgg ccgcggccga cacgcaggtt ttctaccacc
     6601 gcaacctcgc gacctatttc ctcaagcggc gcgactacca gcgggcgatc gaggagctgc
     6661 ggcaggcgaa cgcccggcag cggctcccga agacggacga gatgctggcg gaggcatggc
     6721 tcggcctcgg gcgcccggac gaggccggcg cggcgctcaa ggaggcgctg gcggtgcacc
     6781 ccgacctcga tccggagccg gtcctgtggc tggtgcgcct gaccgccgcg ggcgccggcg
     6841 ggccgggagc ggcgcgcgcc gtggcgcgcg actacgccgc gcgcaccgcg aggaagccgg
     6901 gactcgacga cgccatcgcg ggcctcctgc tcgaggcgga cgctcgccgg gaggaggcgc
     6961 tcgcgacgtt ccgccgctcc atggccgtcg atccggcgcg cgccttcgtc gccgagcgtc
     7021 tctacgcact cgaatcgccg gccgatcggc gcgcccgcct gctgccggcg ctgaagcagg
     7081 cgaccgcacg cgatccgcgc ggcgacgccg cctggaacct gatcggcacg atcgaagccg
     7141 agtcggggga catgaaggcg gcactcgccg ccttccggga ggcgaacgat ctggcgccgg
     7201 acgacgcgcg ctacgccgcc aacctcggcg ccgcctcggc gcgcctcgag cgctgggccg
     7261 atgcggcgga ggcgttcgag cgcgcgctgc ggctcgatcc cgctcccggc gcggcgctca
     7321 agctcggatc ggtctaccgc cggcagggcc ggcagcagga ggctctcgcc gccttcgcgc
     7381 gcgcccgcga gctcggagat tccacccccg cgaccttcct cggcatggcg ctggcgcagg
     7441 aggcgctcgg gagacgcggg gaggcgctgc gctcggtcga cgccgggctg gcgctccacc
     7501 cgggggaggc gtccctcacg cggctcagga gcgagatcgc agcgcgcgct cccgctccgc
     7561 cgcctcccgg tgcagcggat cgtccgggtc ggtccgcgcc tgcacgtcct gataggcgcg
     7621 tcgcgcctcg tccacgcggc cgagcgcctc gtaggcgccg ccgagatcga gccaggggcg
     7681 gtgcagccac ggcgcggccc ggatcgcatc ctgcagggcc cgcatccccg cctccgcctg
     7741 gccggccgcg acctgcgccc gcccgagacc tgcgagcagc tccggctccc ccggagcgcg
     7801 gctgagcgcg gcgcgcagct cgtcgatcgc ctcgtccacg gcccccgcct ccaggagcgt
     7861 ctccgccagc gccaggcgac cgtccacgtc gtcgagccgc gccgccgcgg cggcgcggta
     7921 cgcctcgatc gccccgtccc gatcgccgag cacgcgacgg caggcgccga tctccgcgag
     7981 cacgagtgcc cgcagcggat cctcgtggcc cggagccgcc ccggcctccg accccggggc
     8041 gccgccaggc gcggcggccg cctgaaccgc gcgggcggga tgcgcagagt ccttggccgg
     8101 atcaccggcg cccggcggag cgcccggcgt gagggcggtc gcggccggat cggcggccgc
     8161 cacggcgcgc ggcgtcgtga gctgccggag cgccgcctcg tagagatcga gcgcctggcg
     8221 cgtctccccc aggagccgca gtgttccgcc ggagatcgcc agaagccccg gaggcgtcga
     8281 ccgctccccg cttctctggc gcaggctcct gagatcctcg agcgcgcgcc aggccttcgg
     8341 cccctcccgc gcccgcaacg ccgtcagggc gtcggtcacg agggccgatg cgccgggcgg
     8401 ggacgggatc ggcggcccgg agagcagcgc gtccgcgtcg aacgtcccgg tccccgaccc
     8461 gaacgcctcc ggccactgcc ggccgatctc gacgacccgg tcctgctgca ccgaggccag
     8521 gttgcgcgcc tcccccggat cccgcttcag gtcgtacagc tcgagcgtgt ctccgtggat
     8581 cagcttgaag ccgccgccgc ggatcgccac gcgcgaccgg tcgccgagat agttcgggaa
     8641 cgccaccgcc tcggcgcgcg ccggcagggg cggcatcctc ccctccgggt cgagcaacgg
     8701 cacgaggctc gtcccttcca tcggccgcgg cgccgccaat cgcagcaagt cgagaagggt
     8761 cggcgccaga tcgaagagac gcaccggctc cgggatggag cgatcccgga cgacgagccc
     8821 cgggacgcgc aacaggagcg gaacgcgcag cgtcgcgtcg tacagcccga agccggagcc
     8881 tcgctcggcg tgctcgccca gcccctcgcc gtggctgccg gcgaccacca cgatggtggt
     8941 gccggcgacg ccgagcgagg cgagacgctg cagcagccgg ccgatctccg catccgtgcg
     9001 ctccagctcc gcgtcgtaag ggtcgcgatg ccggagcgtc cgcggcggat cggccgggtg
     9061 cggaggagtc gggtcggaca gctgcaccca caggtagaag cgcgaccgga agtgatcgtc
     9121 cagccaggcg cgcgccgcgt ccaccacgtc ggccgccggg cgctccgcga gccagcggat
     9181 cgtcgccggg cgatccccga cgcggggctc gtcgaagcga tcgaagccgc gcgccagccc
     9241 ggtggcccgc cccaccgccg cggcgccgac gaaggccgcg gtctggtagc ccgcgccgtg
     9301 cagcacctcc gcgagtgtgg cggcgtcggg aggcagcgcc gcgcccggcc gcaggagcca
     9361 cggacgcccg accggcgaag cgccggtcat catcgacagg tgcgccggca gcgtctcggg
     9421 gaccggggtc gtcgcctgct cgaaacggaa cccctcggcc gccaggcggt cgaggtgcgg
     9481 cgtcggcgac agcggcgcgt cgccgtacga accgaggcgg ccgatgcgca ggccgtcgac
     9541 gctgatgagc acgaggctcg gctcctcggg gatcgcgccg tgcccggcgc cgcggcggac
     9601 gcgcttcagt gtcgatccct cgagcggggt caggcgccac gcgagggcgc cgaagagggc
     9661 cgcgaccgcg aagagcgcca ggtaacccag catccagacg gcccgggcgc ggcgggcggc
     9721 ggcgctcatc gatcgccgtg atcgtcgagg aggatcaccg cgacgtcgct ccccgccgga
     9781 agcgacgccg cctccgccgg gacgatggcg agggcgttgc cccgcgcgca ggagacgaga
     9841 tcggccgagc cgctggtggg gaccggctcc acatccaggc gaccgtcccg gaagaccgcc
     9901 cgcgcctgga cgtatccggc gcgcccgggc ttctggcgga tgggcgaagc gaggcgcccc
     9961 ggctgcatcc tccagaccgg ggtcggatgc gacgcgagcc gccgcagcgc cggccgcgcg
    10021 aagacctgga agatgacgta tgacgacacc gggttgccgg ggcacccgaa gacgagagtt
    10081 ccgccgcgac gggcgaaggt gaacggcttt cccggccgga tggcgacccg ctcgaaaagg
    10141 acctcggcgc cggcggaccg gagcgcgccg ccgacgagat cgtagagtcc catcgacacc
    10201 ccgcccgaga ggagcaggac gtccgcgtcg agcccggatg cgatcgcccg gcgcagatcc
    10261 tcctcgtcgt cgcgggcgat gccgaggtcg cgcacttcgg ccccggcgcg gcgggcgagc
    10321 gcctcgagca tggggccgtt gctgttgcgc aggcggcccg gccccggctc ctcgccggga
    10381 ggcaccagct cgtcgccggt cgcgagcacc gcgacgcgcg ggcgcccgcc ggcgcgcgcc
    10441 cggatccgac cgcacgccgc gaggaccgcg atctccgcgg cgccgagaaa gtctccgggt
    10501 tcgaggagca ccgcgccctc gcgcacgtcg tccccgcggc gggcgacgtt ctcgccgggg
    10561 cgcaccggcc cctgcagggc cgtccggtcc ggctgcgatc ccgcccggct ccgctcgacc
    10621 ggtatgacgg cgtcggcgcc gcgtggcagc ggggcgccgg tcatgatgcg cgcggcctgc
    10681 cccggcggaa gcgcggcgag ggcgcccgga tcggcgccgg cggcgatggt cgcggcgatc
    10741 gccacctcct tcccccgccc gagatcggcc gccgccaccg cgaagccgtc catgagcgat
    10801 ttgtcccacg acggcacgtc gcgatcggcg cggatctcct ccagcaggat gcggccgggg
    10861 agatcggcga gatccgcgac gatgggcggg agcggccgcg cggcctcgag gacgaggtcg
    10921 agcgcgcgct cgaccgcgat catgtccggc atgccggcga gtctatcacc cggttccggc
    10981 gggccgccgg agcgccggcc tcggcgcgga cggctcaagc ggcgcgcgcg acgacgacgc
    11041 ggggcaggcc cgccagatcc ggccggatct cctcgagacg gatcgcctcc tgggtcgcgt
    11101 acagggcgcg gacggccgcc tcctggccgg cgccgatctc gacgaggagc catccccctg
    11161 cggcgaggac acgcggcgcc ccctggagga tgcggcggtg cacggcgagt gcatcggccc
    11221 ccggcgtgag cgcgcggcgg ggctcgtgat cgcggacctc cggggcgagc ccgtcgaggt
    11281 cggcgacggc gacgtacggc gggttgctga ggatcgcgtc gaacgggccg tcggacgtcc
    11341 cggacggcag ggcggcgtag aggtctccga gagcgaaggc gacgcgatcg ctcacgccga
    11401 gcgcttcggc gttcgaccgg gcgacctcga gagcggcctc ggagtcgtcc acggcggtga
    11461 cgcgcgcccc ggggagcgcc cgcgccgctg cgatcgccag gcagccggag ccggtgccga
    11521 gatcgaggag acgcggccgc cccggttcca cagggagcgc ccgctccagg agcgcctcga
    11581 gaatcccctc ggtctcgggg cgcgggatga ggacgtcggg ggtgacccgg aacggaagcg
    11641 accagaactc ctgctgcccg gtcaggtgct ggatcggctc gcgtgccgcg cggcggcgga
    11701 cgagctcccg gtagcggcgg accgcgtccc cctccatcgg acgatcgggg cgggcgtgca
    11761 gggcgccgcg gtcgatcccg aggacgtgcg ccagcaggac ctcggcgtcc cgctccggag
    11821 cgtcgattcc ggcggcggcg aacagcgccg aggcctcgcg gacgagcgtc gcggccacgg
    11881 cgggggtcac gcgccggcgc cgacctcttc cttgagccgc tcggcctggt agtgggccag
    11941 gatcggctcg acgagcggat cgagcgcgcc gtcgagcagg tcgggaagcc ggtgcagggt
    12001 gacgttgacg cggtggtcgg tgacgcggtt ctgcgggaag ttgtacgtgc ggatcttctc
    12061 ggagcgctca ccggtgccga cctggctgcg gcggtcctgc gcgatggcgt cccgctgctt
    12121 ctgctgctcg acctcgagca tccgggaccg cagcaccttc atggcgcgcg ccttgttctt
    12181 gtgctgcgat cgctcgtcct ggcactggac cacgagcccg gtcggcaggt gggtgatccg
    12241 gatcgccgaa taggtcgtgt tgaccccctg accgccgggg ccggtggcgc agaaggtgtc
    12301 gatgcgcagg tccttgtcgt tgatctgaat ctgcacgtcg tccgcctccg gcaggaccgc
    12361 cacggtcgcg gtggaggtgt ggatgcgccc ggccgactcg gtcgccggca cccgctgcac
    12421 ccggtgcacg ccgctctcgt gcttgaggcg gctgtacacc tgcttcccgc tgatcagggc
    12481 gatgacctcc ttgagaccgc cgagaccggt gctgctcagc gtcaggacct cgaccgtcca
    12541 gccgcggcgc tcggcgtagc ggctgtacat gcggtacagc tcggaggcga acagcgacgc
    12601 ttcctcgccg ccggtgccgg cgcggatttc gaggatgacg ttcttctcgt cgttcgggtc
    12661 gcgtggcagc aggagctcgc gcagctcggc gagcagcgcg tcccgcttcg gctcgaggag
    12721 ggcgaggtcc tcgcgcgcca tgccgcgcag gtcgggatcg tcggccaggg actgcgcctc
    12781 gcggatctcc ccctcgatct tctcgtactc gcggtagcgc tcgacgacgc gctccagctc
    12841 ggagtaggcc ttggcgatct gccggtagcg atcgggatcg gcggcgatgc ccgggtcggc
    12901 catctgggcc gcgaaggcct cgaatttggc cgcgatcgtc cgcagccgct cctggagctc
    12961 catgggaacc gcccggtcgg cgtcagacgc cggcggtggt ccccttgcgc ggctttttct
    13021 cgggcttgga gcgggtggcg ccgatgcggc gcgcggcgcg gctgacactg gcggccgggg
    13081 cggcggccgc cgccggaggg gtggcctcct gggtggcctt gtagcgcttt tcgaaccgct
    13141 cgacgcggcc ggccgaatcg atcagcttct gcttgccggt gaagaacggg tggcacttcg
    13201 agcagatctc gagccgcagc tcggggttgg tcgaccgggt ctggaaggtc tccccgcagg
    13261 cgcaggagac cgtgaccacg tggtacttgg ggtggatccc gctcttcatg gcgacatcgc
    13321 tcctggggac gggtgtcccc tgcgttcgtg caactcgggt ctgaatggac cggacattat
    13381 aggggtggcg gggcggctcc cacaaggggc ggagccccga gcccctccgg gcgccctccc
    13441 cggaggggac cccgtgcacg gtctacccca tgctcgacat cgagtcgagg aactcctcgt
    13501 tgctcttgct cttctgcatg cgctcgatga gcagctccat cgcctcgacc ggcgacaggg
    13561 agttgagcac cttgcgcagc acccaggagc gatccagctc cttcttcgac aggagcagct
    13621 cctccttacg ggtcccggag cgggtgatct cgatcgacgg gtagatgcgc cgatcggcga
    13681 gcttccggtc gaggtggatc tccatgttgc cggtcccctt gaactcctcg aagatgacgt
    13741 cgtccatgcg gctgccggtc tcgatcaggg cggtggccat gatggtcagc gagcccccct
    13801 cctcgatgtt gcgggccgcc ccgaagaagc gcttcgggcg ctgcagggcg ttggcgtcga
    13861 cgccgcccga cagcaccttc ccggacggcg ggacgatggt gttgtaggcg cgcgccagcc
    13921 gcgtgaccga gtcgagcagg atgacgacgt ccttgcggtg ctcgaccagg cgcttggcct
    13981 tctcgatcac catctcggcc acctgcacgt ggcgggtcgc cggctcgtcg aaggtcgagg
    14041 agatgacctc gccgttgacc gagcgctgca tgtcggtgac ctcctcgggc cgctcgtcga
    14101 tgagcagcac gatgaggacg acctcggggt ggttctgggt gatgctgttg gcgatcgcct
    14161 gcaggagcat cgtcttgccg gtgcgcggcg ccgccacgat gaggccgcgc tgccccttac
    14221 cgatcgggca gaggatgtcg agcacccgcg acgaaacgtt ctcgcgcgtc gtctccagct
    14281 tgatccgggt gttcggatag agcggcgtga ggttgtcgaa gaagatcttg ttgcgcgcct
    14341 gctccgggtg ctcgaagttg accgcctcga ccttgatcag ggcgaagtag cgctccccct
    14401 ccttcggagg acgcacctgg ccggagatgg tgtcgccggt gtgcaggtcg aacttgcgga
    14461 tctgcgacgg cgacacgtag atgtcgtccg ggcccggcag atagttgtac tcgggagcgc
    14521 gcaggaaccc gaagccgtcc ggcagggtct ccagcactcc ctcggcgaag atgagcccgc
    14581 tcttctcggt ctgggcgcgg aggatctcga agatcaactc ctgcttgcgg agcgccgtgg
    14641 cgttcgccac gtcgagcgcc ttggcgatgc cggtcagctc ggagaccgac ttctccttga
    14701 ggacccggat gtcgagcttc tcgccggaca gctcggcgcg cgggacgtcg tcgcgctcga
    14761 cctcgtcgtc gaccatcgcc ccgacggcga cgtcggcctg cgggggcggt ccggccggcg
    14821 ggtccatcgg cggacggcgg ggcggccggc cccctgcgtc gcgtcccggt ccgcggggcg
    14881 gacggggctc gcggggacct cgattgtcac gctcgatgtc tttgccgttc acggtggtgt
    14941 ccttctccat gtgggtgagg gggaacgagg ggttcggcgc cgggggaagc gttgcggccc
    15001 cgctcgcagg cggcgtcgag tatacgccag agggcgggga ttccgtcacc ggtcacggcg
    15061 gagacgggga cgggcggttc aggttcggac tggaacgccg ccgcagcggc gcgggtgacc
    15121 gcagcccacc ggccgcgggg gagcttgtcg atcttggtca gcgcgatacg ggcagggcgg
    15181 tgggcggcgg acagccagtc ccacagctcc aggtccaggt cggtcggcgg gtgccgcgcg
    15241 tccaccagca ggatcgacag gtccggcccc ggggctccct cgaggtacgc ctcgacgagc
//
LOCUS       DASRIQ010000057         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3142391, whole genome shotgun sequence.
ACCESSION   DASRIQ010000057 DASRIQ010000000
VERSION     DASRIQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3142391"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFD06_01490"
     CDS             <1..308
                     /locus_tag="VFD06_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02237.1"
                     /translation="IFFSAIGVALVLTFVDPRTFIALIPAVLITWILLGMPLDRFFRW
                     RKIGKGDWFLPGISLGSGSYSSGGYSGSSGYSSSSSSSSSYSGGGGRFGGGGASGGW"
     gene            complement(342..1088)
                     /locus_tag="VFD06_01495"
     CDS             complement(342..1088)
                     /locus_tag="VFD06_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase"
                     /protein_id="HZN02238.1"
                     /translation="MKNAFQLVASLAVVVAAASTPCAYATVYFQNTGTLSGWTGVSVG
                     ETGSVTQVSSPTYKGSTALRCYQIYNGADKTLHSEVSYGNADVGRNGDNRYYGFAFRT
                     HSNWTYATPGASISQLITNSPCGAQQTDMYQIYGARLEVKRNWGDPCNQGYERLTIVD
                     PVSVGIWHRVVIRKLWKTDNTGAWQVWYDGSRKHSKTNIPTGFTGDPPYRWTIGLYAG
                     FSSTSPKTRTIYVDHARVASSYSEADPGQW"
     gene            complement(1286..>2336)
                     /locus_tag="VFD06_01500"
     CDS             complement(1286..>2336)
                     /locus_tag="VFD06_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02239.1"
                     /translation="KLDHHFLAEGRAEPLVIELPKGSYMPVFRPRVEPPTADSAAAAA
                     EPMAIAPAPEPRATVRWPVLSFGLLLVVAAGVLMTRQSLRPASSPGASPFRDHFWRQF
                     VAGRETQVLLSDASVTMLAELRGRDVTLAEYSQGSYPGSVIADLDLPPKVRAVMEQAA
                     VKGFTNVPDTFVADNVAQMCRLHHPAVHIVSARDLKPDLARSENLVVLGHRRANPWGS
                     LFETSLDFTYRFDPATRTASIVNRAPQAGEKPAYVVDWGRRGYCVVAYLPKPRGEGNV
                     LLVFGTDWVSVRAGGLFVTDEPALRGVHERLGAGLSEPIPFFAVLLQAELAGNVTTDY
                     RPIAHRRVGPPAGER"
BASE COUNT          379 a          737 c          821 g          399 t
ORIGIN      
        1 ggatcttctt ctccgccatc ggcgtcgcgc tggtcttgac cttcgtggac ccgcgcacgt
       61 tcatcgccct catcccggca gtgctcatca cgtggatcct gctcggcatg ccgctcgatc
      121 gtttcttccg ctggcggaag atcggcaagg gcgactggtt cctccctggg atcagcctgg
      181 gctcgggcag ctattcgtcg gggggctact cggggtcgtc gggctatagc tcatcctcgt
      241 cctcgtcttc gagctattcc ggcggcggcg ggcgcttcgg cgggggcggg gcttccggcg
      301 gctggtaggc gaggctcaag gcgatcttcg aatcactaga tctaccactg ccccgggtcg
      361 gcttcgctgt aggaagaggc gacccgcgca tgatcgacgt agatcgtgcg ggtcttcggg
      421 ctcgtgctgc tgaatccggc gtagagtccg atcgtccatc gatacggagg atcgccggtg
      481 aaccccgtgg ggatgttggt cttgctgtgc ttgcgtgagc cgtcgtacca gacctgccac
      541 gcgcccgtgt tgtcggtctt ccagagctta cggatcacca cgcgatgcca gatgcccacg
      601 ctgacggggt ccacgatggt gagcctctcg tagccctgat tgcacggatc gccccagttg
      661 cgcttgactt ccagtcgagc tccatagatc tgatacatgt cggtctgctg cgcgccacac
      721 ggcgagttgg taatgagctg cgaaatggag gctcccgggg tggcatacgt ccagttgctg
      781 tgcgtgcgga acgcaaagcc gtagtagcga ttgtcgccat tgcgcccgac gtcggcgttc
      841 ccgtaggaca cctccgagtg aagggtctta tcggcgccgt tgtagatctg ataacaccgg
      901 agggcagtcg atcccttgta ggtcgggctg ctgacctggg tcacggaacc ggtttccccg
      961 acggaaacgc cggtccaacc cgaaagcgtt ccggtgttct gaaaatagac ggtggcgtag
     1021 gcgcacggcg tcgatgccgc cgccaccacc acggccagac tggccacgag ctgaaaagcg
     1081 ttcttcatgt cggcctcctg tcgttgagat gtggggctca cgttaggcgg ccccggggca
     1141 gtggagaagg atcgttcggc gcaggccgcg agggcgcgga tggttcagag aaggcggacc
     1201 ggttgcaggc tcagccgctt gcgagtcgcc cggggcgggc cgacccacga gaatcgcgcg
     1261 agaatcgcgc ccgctggcgg tccgatcagc gctcgccggc cggcggcccg acgcgacgat
     1321 gggcgatggg ccgatagtcc gtggtcacgt tgcccgccag ctcggcctgg aggaggacgg
     1381 cgaagaacgg gatcggctcc gaaaggccgg cgcccagacg ctcgtgcacg ccgcggaggg
     1441 ccggctcgtc ggtgacgaac aggccgccgg cacggacgga cacccagtcg gtcccgaaaa
     1501 cgaggaggac gttgccctcg cctcggggct tgggcagata ggccaccacg caatagcccc
     1561 gccgtcccca gtcgacgacg taggccggct tctcgccggc ctgcggcgcg cggttgacga
     1621 tgctggcggt ccgagtggcc ggatcgaagc gataggtgaa gtcgagggag gtctcgaaca
     1681 atgagcccca cggattggcg cgccggtgtc cgaggacaac gaggttttcg ctgcgcgcga
     1741 ggtcgggctt gaggtcacga gcggagacga tgtgtaccgc cgggtggtgg agccggcaca
     1801 tctgcgcgac gttgtcggcc acgaatgtat cgggcacgtt cgtgaaaccc ttcaccgcgg
     1861 cctgttccat caccgccctc accttcggcg gcagatcgag atcggcgatg acgctcccgg
     1921 gataggagcc ctggctgtac tcggccaggg tcacgtcgcg gccgcgcagc tccgccagca
     1981 tggtcacgct ggcgtcggac aacaggacct gcgtctcgcg gccggcgacg aactgccgcc
     2041 agaaatggtc ccggaagggt gaagcgccag gagaagacgc agggcgaagg gactgcctcg
     2101 tcatcaagac gcccgcggcc acgacgagca ggagcccgaa agacaggacg ggccaccgga
     2161 ccgtcgcacg gggttctggc gccggcgcga tcgccatggg ctccgcggcc gcggcggcgc
     2221 tatcggcggt cggcggctcc acgcgaggcc ggaagaccgg catataggag cccttcggca
     2281 gctcgatgac cagcggctcc gcccgtccct cggccaggaa gtggtggtcc agcttg
//
LOCUS       DASRIQ010000058         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1331676, whole genome shotgun sequence.
ACCESSION   DASRIQ010000058 DASRIQ010000000
VERSION     DASRIQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331676"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VFD06_01505"
     CDS             complement(<1..705)
                     /locus_tag="VFD06_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN02240.1"
                     /translation="MLAALPLASMPAAADEATAMAAAGRHVAAQEAAIVGELRELLAL
                     PNVATSATDIRANADLLVRMLGKRGVAARLLETPGAPVAVYGERRVPGAKRTLLFYAH
                     FDGQPVAPESAWMSPPFEPVLRAGRAEDGAPVIPWSEAKFPLPDEARIYARSSSDDKG
                     PIVAMLAALDALDAGRIPLSANLKFFLEGEEEAGSPNLARTLAAHRGLLGSDLWIFGD
                     GPIDPRGLPRLSLGVRG"
     gene            849..1385
                     /locus_tag="VFD06_01510"
     CDS             849..1385
                     /locus_tag="VFD06_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HZN02241.1"
                     /translation="MAGLLVSVSASAADPAPPATAPSTAASVGLFVYAAKGQDAAQQS
                     KDESECYTWATQNTGTDPFALQKQSQAQAQQTEQQKQQAQKAGAGAGAGGAVKGAAAG
                     ALIGEIANDDAGEGAAVGAAVGVIAGRRRGREAKKQATAQAEAQGQQAQAATAEQMTN
                     FKKAFSVCLEAKKYMVKY"
     gene            complement(1429..2160)
                     /locus_tag="VFD06_01515"
     CDS             complement(1429..2160)
                     /locus_tag="VFD06_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZN02242.1"
                     /translation="MVRLPKLSLKSRLARFWDEDRGLSALLAFLAVIAFIVAPLAETT
                     RGGGTVLRACVSLMFAFGAYVLSPRRRDRLVVALLAAAPILLGWMEYVRPAPLWQAAA
                     ALSSCLFALTLAAFVTLRVFQEGPITIHRVVGSIAVYLMIGIAFAEAARCVRIVDPGA
                     YASASFSRIDRPELYYFSLVTLSTVGYGDITPVHPFARSLAVLEALIGQLFPAILIAR
                     LVSQELMGHHARRPEPGSRRPEPDD"
     gene            2265..2945
                     /locus_tag="VFD06_01520"
     CDS             2265..2945
                     /locus_tag="VFD06_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02243.1"
                     /translation="MTNPRRLPSSRSAASRPRRRPADLLVAAALACGLALPGLPTSPA
                     RADAPALMDTWRGPGSARPEFKKVLVVGITRNAPARRRFEDLFVSILRGRESQAITSY
                     SLAPDLDNIPDPEKVVETLFAGQVDGVITVRLVSLDGRDEAQWGEAWRKELDEPVRIR
                     PYVTDSLRRLAPDADLHGAEFALWDVQTGERLWAGRSGPVKVKKLKKSATDLVQDVMT
                     ELRFRALL"
     gene            complement(2949..>4568)
                     /locus_tag="VFD06_01525"
     CDS             complement(2949..>4568)
                     /locus_tag="VFD06_01525"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HZN02244.1"
                     /translation="DPYIEPVLDLRVTKGTFFADGDFQANLLDMEHPRVSFQGNVRMD
                     DFAAIDGRDREELLRWGSVRLDRIDYSLEQDRMRIGDLTIAGGRAALVVAPDGTINLA
                     RVLRLPPPVPVAEENADAATENPGEAGSPEAGSAPAAAAAAADGGDTRIARARLRDGQ
                     IRLIDRSMTPAAELLLDRIDGTLAGLSSRPGARADVHLKALVGETAPLSLDGQVDPLG
                     AEVFSDLVLTAEGIDLAPLGPYSARYIGYELDRAKLSLDLRYRLESRAVAGTNVFTAD
                     PFLLGGKTDSPDATKLPVRLGLALLRDRHGVIRLDVPVEGSLDDPKFRLGRVILRALV
                     NVFTKLVTAPFTLLARAFAGRDDIDLSLIEFAPGSAAIAEGERPRLETLVKGLTDRPG
                     LSLAITGRVAPDPGGAGSDFDALRRAKLNGLVRAAKWKSLRRAARDVTPVASVVVEPG
                     EAPRFLKAAWKEFLEAHPDAAGDPKPETPEEIEASLLPRLEVGAEDLTSLAAARAAAV
                     RDHLIAAGVETTRLEVKTEPAGGAKVTLELQ"
BASE COUNT          691 a         1589 c         1709 g          579 t
ORIGIN      
        1 gccccgcacc cccagcgaca ggcgcggcag gccccgcgga tcgatcgggc cgtcgccgaa
       61 gatccacagg tcggaaccga gcaatccgcg gtgcgccgcg agcgtgcgcg cgaggttcgg
      121 cgaacccgcc tcttcctcgc cctcgaggaa gaacttgagg ttcgcggaga gcgggatcct
      181 tccggcgtcg agcgcatcga gggccgcgag catggcgacg atcgggccct tgtcgtcgct
      241 cgaggagcgc gcgtagatgc gggcctcgtc gggcagcggg aacttcgcct cgctccaggg
      301 aatcaccggc gccccgtctt cggcgcggcc tgcgcgaagc acgggctcga agggcggcga
      361 catccacgcg ctttccgggg cgaccggctg cccgtcgaaa tgcgcataaa aaaggagcgt
      421 gcgcttcgcg cccggcacgc gccgctcgcc gtagacggcc acgggcgcgc cgggcgtctc
      481 gaggaggcgc gcggcgacgc cgcgtttccc gagcatccgc accaggagat ccgcgttcgc
      541 gcggatgtcg gtggcgctcg tcgcgacgtt cggcagcgcg aggagctcgc gcagctcgcc
      601 gacgattgcg gcctcctgtg ccgcgacgtg ccggccggcg gccgccatgg ccgtggcctc
      661 gtcggccgcg gcaggcatgg atgccagcgg cagcgccgcc agcacgcccg cagcaatgag
      721 atgcatcgtc atgcccggat gatcgggcgc atcgcgtggc gccgcaaccg gagtacgatg
      781 gcgggccggc gcatcaacga cgcccgaagg aggccgtgat ggcaagttgc aggttcccgc
      841 ttctcggaat ggcaggcctc ctggtctcgg tgtccgcgag cgcggccgac ccggccccgc
      901 ccgccacggc cccatcaacg gcggcatcgg tcggcctttt cgtctatgcg gcgaaaggac
      961 aggacgccgc ccagcagtcg aaggacgaat ccgagtgcta cacctgggcg acgcagaaca
     1021 ccgggaccga tccgttcgcc ctgcagaagc agtcccaggc gcaggcgcag cagaccgagc
     1081 agcagaagca gcaggcccag aaggcggggg ccggcgccgg ggcgggaggg gcggtgaagg
     1141 gtgcggccgc gggggcgctc atcggcgaga tcgccaacga cgatgccgga gaaggtgccg
     1201 ccgtcggcgc cgccgtgggg gtgatcgccg gccggcgtcg cggacgcgag gcgaagaagc
     1261 aggcgacggc gcaggcggaa gcgcagggac agcaggcgca ggcggcgacc gccgagcaga
     1321 tgaccaattt caagaaggcg ttcagcgtct gcctcgaagc caagaagtac atggtcaagt
     1381 actgacggcg gggccgccgc ggtgcggccg cggggctgcg cgggctgact aatcgtccgg
     1441 ctccgggcgc cgcgagcccg gctcggggcg ccgcgcgtga tgccccatca gctcctggga
     1501 caccaggcgc gcgatcagga tggcggggaa gagctgcccg atgagcgctt cgagcaccgc
     1561 cagggaccgg gcgaaggggt ggaccggggt gatgtcgccg tagccgacgg tcgacagggt
     1621 gacgaggctg aagtagtaca gctcgggccg atcgatgcgc gagaacgagg ccgaggcgta
     1681 ggcccccgga tcgacgatcc ggacgcagcg cgcagcctcg gcgaacgcga tgccgatcat
     1741 caggtagacg gcgatcgagc cgaccacccg gtggatggtg atcgggcctt cctggaacac
     1801 ccgcagcgtg acgaatgccg cgagggtcag cgcgaacagg cacgaggaca aggcggcggc
     1861 cgcctgccac aggggcgccg ggcgcacgta ttccatccac ccgaggagga tcggcgccgc
     1921 ggcgagcagg gccacgacga ggcggtcgcg acgccggggg gacaggacgt aggcgccgaa
     1981 ggcgaacatc agcgagacgc acgcccgcag aaccgtgccg ccgccgcgcg tggtctcggc
     2041 gagcggcgcg acgatgaacg cgatgacggc gaggaacgcc agcagggcgc tgaggccgcg
     2101 atcctcgtcc cagaatcggg cgaggcgcga tttcagggag agcttcggaa gtcgaaccac
     2161 ggcttcggcc ctcgcgggaa cgacggcggg cgcggacggg ccgaggctat cacgccccgc
     2221 gggccgaagg cgcgatgcta tgatcggccg ctctcgaagc gaccatgacg aatccccgca
     2281 ggctcccgtc atcccgcagc gcagcgtcgc gcccccgccg gcgtccggcg gatctgctcg
     2341 tcgccgcggc gctcgcgtgc ggcctcgcgt tgccggggct cccgacctcg cccgcccggg
     2401 ccgacgcccc agccctgatg gacacatgga gggggcccgg ttcggcgcgg ccggagttca
     2461 agaaggtcct ggtcgtcggc atcacccgca acgccccggc ccgccgccgc ttcgaggacc
     2521 tgttcgtcag catcctgcgc ggccgcgaat cgcaggcgat cacgagctat tctctcgccc
     2581 ccgatctcga caatatcccc gaccccgaga aggtcgtcga gacgctgttc gccggccagg
     2641 tggacggcgt gatcaccgtg cggctcgtgt cgctcgacgg ccgggacgag gcgcaatggg
     2701 gggaggcctg gcgcaaggag ctcgacgagc cggtgcgcat ccgcccctac gtcaccgact
     2761 cgctgcggcg gctcgccccc gatgccgatt tgcacggggc ggagttcgcg ctgtgggatg
     2821 tccagaccgg cgagcggctc tgggcagggc gcagcggtcc ggtgaaggtc aagaagctaa
     2881 agaagagcgc caccgacctg gtgcaggacg tcatgacgga gctgcgcttc cgcgcgctcc
     2941 tttgaggcct actgcagctc gagcgtgacc ttggccccgc cggccggttc ggtcttcacc
     3001 tccaggcgcg tggtttcgac gccggcggcg atcaggtggt cgcgcacggc ggcggcgcgc
     3061 gccgcggcca gcgatgtgag gtcctccgcc ccgacctcga ggcgcggcag caggctcgcc
     3121 tcgatctcct cgggcgtctc cggcttcggg tcgcccgccg cgtcggggtg ggcttcgagg
     3181 aattccttcc acgcggcctt gaggaagcgc ggggcctccc ccggctcgac gaccacgctc
     3241 gccacgggcg tgacgtcgcg cgccgcgcgc cgcagcgatt tccatttcgc ggcgcggacc
     3301 aggccgttca gcttcgcacg ccgcagcgcg tcgaagtccg agccggcccc accgggatcg
     3361 ggcgcgacgc ggccggtgat cgccagggac agtccgggac ggtcggtgag ccccttcacg
     3421 agggtctcga gccgcggccg ctccccctcg gcgatcgccg ccgagccggg ggcgaactcg
     3481 atgaggctga ggtcgatgtc gtcgcggccg gcgaaggcgc gcgccagcag cgtgaacggc
     3541 gccgtgacca gcttggtgaa gacgttcacg agagcccgca ggatgacgcg gccgagccgg
     3601 aacttcgggt cgtcgagcga cccctccacc ggcacgtcga gcctgatgac accgtgccga
     3661 tcgcgcagca gcgccagacc caggcgcacc ggaagcttcg tggcgtcggg gctgtcggtc
     3721 ttccccccga gcaggaaggg atcggcggtg aagacgttgg tccccgcgac ggcgcggctt
     3781 tccagccggt agcgcaggtc gaggctgagc ttcgcccggt cgagctcgta gccgatgtag
     3841 cgcgcgctgt acggcccgag cggtgcgagg tcgatcccct cggcggtcag cacgagatcg
     3901 ctgaagacct cggcgccgag cgggtcgacc tggccgtcga ggctcagggg agccgtctcg
     3961 ccgaccagcg ctttcaggtg gacgtcggca cgtgccccgg ggcgcgacga cagacctgcg
     4021 agcgtgccgt cgatgcggtc gagcaggagc tccgccgcgg gggtcatgct gcgatcgatc
     4081 aggcggatct gcccgtcccg gagccgtgcc cgggcgatgc gcgtgtcgcc gccgtccgcg
     4141 gcggccgcgg cggccgcggg ggcgctgccg gcctccggcg atcccgcttc gcccggattt
     4201 tcggtcgcgg cgtccgcgtt ctcctccgcg accgggacgg ggggcgggag ccggagcacg
     4261 cgcgcgagat tgatcgtccc gtccggcgcc acgacgagcg ccgcgcgccc cccggcgatg
     4321 gtcaggtcgc cgatgcgcat gcgatcctgc tccagcgagt agtcgatccg gtccagccgg
     4381 acggatcccc agcgcaggag ctcctcgcgg tcgcggccgt cgatcgccgc gaagtcgtcc
     4441 atgcgcacgt tcccctggaa cgacacgcgc gggtgctcca tgtcgagcag gttcgcctgg
     4501 aaatcgccgt ccgcgaagaa ggtccctttc gtgacgcgca gatcgagcac cggctcgatg
     4561 tacggatc
//
LOCUS       DASRIQ010000059         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_22755, whole genome shotgun sequence.
ACCESSION   DASRIQ010000059 DASRIQ010000000
VERSION     DASRIQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22755"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /gene="alaS"
                     /locus_tag="VFD06_01530"
     CDS             complement(<1..1985)
                     /gene="alaS"
                     /locus_tag="VFD06_01530"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZN02245.1"
                     /translation="MTSANDIRRRFLAFFEGLGHQVVPSSPLIPAQDPTLLFTNAGMN
                     QFKDTFLGREQRPYARAASSQKCMRVSGKHNDLEQVGRTPRHHTFFEMLGNFSFGDYF
                     KKDAIAYAWDLLTSSKEYGLPKEKLFVTVFREDDEAHELWRKGPGVPESRIARLDEAD
                     NFWSMGETGPCGPCSEIHVDRGKQPIPCNVSPCRPGCDCGRFMEIWNLVFMQYDRRPD
                     GTLVPLARTGVDTGMGLERLSAVLQGVESNYDTDLFTPIVVAAARLAEEASGRRPDPS
                     RGDDRVGLQVIADHVRAVAFLVGDGVVPGNDGRGYVLRRILRRAIRFGRALGIERPFL
                     GDLTGTVIDVMESAYPDLSAHRTSIAAAARREEERFAGTLAVGLQKVEALAGSLRKEG
                     KGTIPGAEAFRLYDTFGVPLDLVKDVALAWRLEVDQAGFEREMEEQRSRSRRGMKEVA
                     APAAGALAQAPIASSEFAGYIEHVVEQARIVALLRGDALVDELRHGEDGGVLLDRTPF
                     YAEAGGQVGDTGTLTASGGAAAVTDTQAPRRGLPLHMVRVLEGRLRVGEPVRAEVNRE
                     RRLATTRAHDATHLVHAALREVVGTHVKQAGSLVEADRLRFDFTHYAALPEGIQRQVE
                     DLVNDVVRQDLPIRSAVLPLDEALRRGALAFFGDKYA"
     gene            complement(2080..3276)
                     /locus_tag="VFD06_01535"
     CDS             complement(2080..3276)
                     /locus_tag="VFD06_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZN02246.1"
                     /translation="MHINDLLKTATERKASDLHLKVGSYPVIRVSGHLLALTELARLS
                     QEDTIAMAFSIMSGRQKQKFKDHFEIDLAYSVPGLGRFRVNIFQQRGTVGLVLRVIPS
                     KILTTEELVLPPVLKTVADERRGLILVTGTTGSGKSTSLAAMIDYINTTRTEHIMTIE
                     DPIEFLHRDKKSLVNQREVEVDTKSFASALRSALRQDPDVILVGEMRDYETIETALNA
                     AETGHLVLSTLHTLDATETINRIISVFPPHQQKQIRLQLAAVLKAAISQRLMPRADGN
                     GRVPAVEVMRTTNYIRDCVENKDKTKLIHDAIAQGTSQYGMQTFDQSIYQLYKKDLIT
                     MEEALRRATNPDEFKLKLAGIESTAEMAQREMESTLRTGFEAGAPPGGGGSVVELSRA
                     HAGKKK"
     gene            complement(3396..4355)
                     /locus_tag="VFD06_01540"
     CDS             complement(3396..4355)
                     /locus_tag="VFD06_01540"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZN02247.1"
                     /translation="MPFAKTDKVWMNGKLVPWDQATVHVCSHVIHYGSCIFEGIRCYK
                     TPQGPAIFRLKEHIERLFDGCAIYRMEVPYSRETIEQAVVETVRVNKMDACYIRPLAY
                     RGYESLGVDPFPCPIDVAVAVWPWGAYLGKDATEKGVDAMVSSWRRQAPDTLPAMAKA
                     SANYMNSQLIKMEALANGYVEGIALDSYGFVSEGSGENLFVVKRGQLYTPPLTASILP
                     GITRASILTIAADLGLTVVERSVPREMLYISDEVFFVGTATEVTPVRSVDRHVIGNGR
                     RGEITARIQAEYMALIQGEKPDRHGWLTHVPALAAAASVRQRG"
     gene            complement(4474..>4846)
                     /locus_tag="VFD06_01545"
     CDS             complement(4474..>4846)
                     /locus_tag="VFD06_01545"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZN02248.1"
                     /translation="LGAASAAGIDADPVAVAVARQTRRMNRIGPGLTLRRADASSALD
                     PRRFDLALVNIGARVIRDLLPRLSRALRPGGRAILAGILIEDEPDLLRRARRAGLRLA
                     ARRRTRPWSALLLVKRSVTPS"
BASE COUNT          705 a         1669 c         1706 g          766 t
ORIGIN      
        1 tcggcgtact tgtccccgaa gaacgcgagc gctccgcgac gaagcgcctc gtcgagcggc
       61 aggacggcgg agcggatcgg caggtcctgg cggacgacgt cgttgaccag gtcttccacc
      121 tggcgctgga tcccctcggg cagggccgca tagtgcgtga agtcgaagcg caggcgatcc
      181 gcctcgacga gcgagccggc ctgcttcacg tgcgtgccga ccacctcccg gagcgccgcg
      241 tgcacgaggt gcgtggcgtc gtgggcgcgc gtcgtcgcca gccggcgctc gcggttcacc
      301 tcggcccgca ccggttcgcc gacgcgcagc cgcccctcca gcacccgcac catgtgcagc
      361 ggcagcccgc ggcgcggcgc ctgggtgtcg gtgacggcgg cggcgccgcc ggaggcagtg
      421 agggtgccgg tgtcgccgac ctgtcccccc gcctcggcat agaacggcgt gcgatcgagg
      481 agcactccac cgtcctcgcc gtgccgcagc tcgtccacca gcgcgtcgcc acgcagcagc
      541 gccacgatgc gcgcctgctc gaccacgtgc tcgatgtagc cggcgaattc gctcgacgcg
      601 atcggcgcct gcgccagcgc cccggcggcc ggtgcggcca cctccttcat gccgcgccgc
      661 gaccgggagc gctgctcctc catctcccgc tcgaatccgg cctgatccac ctcaagcctc
      721 cacgccaggg cgacatcctt caccaggtcg agcggcacgc cgaaggtgtc gtagagccgg
      781 aacgcctccg ccccggggat ggtccccttc ccctccttgc gcaggctgcc ggcgagtgcc
      841 tcgaccttct gcaggccgac cgcgagggtc ccggcgaagc gctcctcctc ccgccgcgcg
      901 gcggcggcga tcgaggtccg gtgggcggac agatccgggt acgccgactc catcacgtcg
      961 atcaccgtcc cggtgaggtc gccgaggaag ggccgctcga tcccgagggc ccgtccgaag
     1021 cggatggccc gcctgagaat ccgacgcagc acgtagccgc ggccgtcgtt gccgggaacc
     1081 accccgtcgc cgaccaggaa ggccacggcc cgcacgtgat cggcgatcac ctggagcccg
     1141 acccggtcgt cgccgcgcga cggatcggga cgtcggccgg aggcctcctc ggccaggcgc
     1201 gccgcggcga cgacgatcgg cgtgaagagg tcggtgtcgt agttgctctc gaccccctgc
     1261 agcaccgccg acaggcgctc cagtcccatc ccggtatcca cgccggtgcg ggcgagcggc
     1321 acgagagtcc cgtcgggccg ccggtcgtac tgcatgaaga cgaggttcca gatctccatg
     1381 aagcggccgc aatcgcagcc ggggcggcag ggcgaaacgt tgcaggggat cggctgcttg
     1441 ccccgatcga cgtggatctc ggagcacgga ccgcacgggc cggtctcccc catcgaccag
     1501 aagttgtccg cctcgtcgag ccgcgcgatg cgcgactcgg gcactcccgg ccccttcctc
     1561 cagagctcgt gcgcctcgtc gtcctcgcgg aagacggtga cgaagagctt ctccttgggg
     1621 aggccatact ccttcgagga ggtcagcagg tcccaggcgt aggcgatcgc gtccttcttg
     1681 aagtagtcgc cgaaggagaa gttccccagc atctcgaaga aggtgtggtg ccgcggcgtc
     1741 cgaccgacct gctcgaggtc gttgtgcttc cccgacacgc gcatgcactt ctgcgaagag
     1801 gcggcgcgcg cgtacggccg ctgctcgcgc ccgaggaacg tgtccttgaa ctgattcatg
     1861 ccggcgttgg tgaacaggag ggtcggatcc tgggcgggga tcagcggcga cgaggggacg
     1921 acctggtggc cgagcccttc gaagaaggcg agaaaccgcc tgcggatgtc gttggccgac
     1981 gtcatgtcgc gtggtccttc cggcgccccg tgaacccggc gccgcgacgg gcccgtgagt
     2041 atagcaagag gccccggcct ccccgcgggc gcggcggacc tacttcttct tcccggcgtg
     2101 ggcgcgggac agctcgacca ccgatccccc gccccccgga ggtgcgcccg cctcgaagcc
     2161 ggtgcgcagc gtgctctcca tctcccgctg cgccatctcg gcggtcgact cgatgccggc
     2221 gagcttgagc ttgaactcgt ccgggttggt ggcgcgcctc agggcctcct ccatcgtgat
     2281 cagatccttc ttgtagagct ggtagatcga ctggtcgaag gtctgcatgc cgtactgcga
     2341 ggtcccctgc gcgatggcgt cgtggatgag cttggtcttg tccttgttct cgacgcagtc
     2401 gcggatgtag ttggtggtgc gcatcacctc gaccgcgggg acccgcccgt tgccgtcggc
     2461 gcgcggcatg aggcgctgcg agatcgccgc cttgagcacc gcggccagtt gcagccggat
     2521 ctgcttctgc tggtgcggcg ggaagaccga gatgatgcgg ttgatcgtct cggtcgcgtc
     2581 gagggtgtgc agggtcgaga ggaccaggtg gccggtctcg gcggcgttga gcgccgtctc
     2641 gatcgtctcg tagtcacgca tctcgccgac caggatgaca tcggggtcct ggcgcagggc
     2701 cgagcgcagc gcggaggcga acgacttggt gtcgacctcg acctcgcgct gattgaccag
     2761 gctcttcttg tcgcggtgca ggaactcgat cggatcctcg atcgtcatga tgtgctcggt
     2821 gcgggtggtg ttgatgtagt cgatcatcgc ggcgagcgag gtcgacttcc ccgaaccggt
     2881 ggtgccggtc accaggatga ggccgcgccg ctcgtcggcg acggtcttga gcaccggcgg
     2941 caggaccagc tcctcggtcg tgagaatctt cgaggggatg acgcgcagca ccagccccac
     3001 ggtgccgcgc tgctggaaga tgttgacgcg gaagcggccg agcccgggaa ccgagtaggc
     3061 cagatcgatc tcgaagtggt ccttgaactt ctgcttctgc cgcccgctca tgatgctgaa
     3121 ggccatggcg atggtgtctt cctgcgacag ccgggccagc tcggtgaggg ccagcaggtg
     3181 gccgctgacg cggatcaccg ggtacgagcc gaccttgaga tggaggtcgg aggccttgcg
     3241 ctccgtggcg gtcttcagca ggtcgttgat gtgcatcgcg tctcccccgg cggtcctcgg
     3301 acacacgacc ggcgcggtcg ggggggaatc tagggtccgc ccccggggga gtcaaggaat
     3361 tggctgtgcc gtaaggactt gaacccgcgg gcggcctacc cgcgctgccg gacggacgcg
     3421 gccgcggcga gcgccgggac gtgcgtcagc cagccgtggc ggtccggctt ctccccctgg
     3481 atgagggcca tgtactccgc ttggatccgc gcggtgatct cgccccgccg gccgttgccg
     3541 atgacgtgcc ggtcgacgct gcgcaccggg gtcacctcgg tggcggtccc gacgaagaac
     3601 acctcgtcgg agatgtacag catctccctg ggcacgctcc gctcgacgac cgtgagcccg
     3661 aggtccgcgg cgatggtcag gatgctcgcg cgggtgatgc cgggcaggat cgacgcggtg
     3721 agcggcggcg tgtagagctg gccgcgcttc acgacgaaaa ggttctcgcc cgacccctcg
     3781 ctgacaaatc cgtaactgtc gagggcgatc ccctcgacgt agccgttcgc cagcgcctcc
     3841 atcttgatga gctgcgagtt catgtagttg gcgctcgcct tcgccatcgc cgggagggtg
     3901 tccggggcct gccggcgcca cgaggagacc atggcgtcca cccccttctc ggtcgcgtcc
     3961 ttcccgaggt aggcgcccca cggccagacc gccacggcca cgtcgatcgg gcacgggaac
     4021 ggatcgacgc cgagcgactc gtagccgcgg tacgccagcg gccggatgta gcaggcgtcc
     4081 atcttgttca cccggacggt ctcgaccacc gcctgctcga tggtctcgcg gctgtacggg
     4141 acctccatcc ggtagatggc gcagccgtcg aacagccgct cgatgtgctc cttgaggcgg
     4201 aagatggccg gcccctgcgg cgtcttgtag cagcggatgc cctcgaagat gcaggagccg
     4261 tagtggatga cgtgcgagca gacgtgcacc gtcgcctggt cccatggaac cagcttgccg
     4321 ttcatccaga ctttgtcggt ttttgcgaag ggcatcttct tcacctcgcg gcggggggtg
     4381 agcgaacagc gcgcagtata aaacaggtcc cgcacgtccg aacagcacaa atccaagggc
     4441 gtacccgtcc aatatgaggg ttgcctcggt cctctaggac ggggtgacgg accgtttcac
     4501 gaggagcagc gcggaccagg ggcgcgtgcg ccgccgtgcc gccagccgca gcccggcccg
     4561 gcgggcgcgc cggagcaggt cgggctcgtc ctcgatcagg atgccggcga ggatcgcccg
     4621 cccaccgggg cggagcgccc gggagagccg cggcagcagg tcgcggatga cgcgggcgcc
     4681 gatgttgaca agcgccaggt cgaagcgccg cgggtcgagc gccgaggacg cgtcggcgcg
     4741 ccgcagggtc agaccgggac cgatccggtt catccggcgc gtctgccgcg ccaccgccac
     4801 cgccaccggg tccgcatcga tccccgcggc gctcgcggcg ccgagg
//
LOCUS       DASRIQ010000060         8508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2891993, whole genome shotgun sequence.
ACCESSION   DASRIQ010000060 DASRIQ010000000
VERSION     DASRIQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2891993"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2210
                     /locus_tag="VFD06_01550"
     CDS             <1..2210
                     /locus_tag="VFD06_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZN02249.1"
                     /translation="VSLHVTGIPDIDAVPASLTFPTTFIGFSSSLPLSLRNPGTDVLH
                     IASLDVSGDYSVSGLTAPAAIPAGGAIPLVVTFTPTGAGPRSGAVAIASDDPDESPFV
                     VSLSGAALVPPAIGVDPEAIATALPPAGARTKSLTLSNTGGSDLVWSLGTVRLSTTVP
                     PGTYLALGKGEEDPRLGILGSGGPDAFGYVFKDSDEPDGPAFAWTDISGVGTPIAALT
                     GDDQNAGPIAIGFPFPFYGNTFTSVRVATNGWLSFTSTSTAHINQPLPNTASAVPENL
                     VAGLWDDLHFRGAVRAHSYNDGSRFIVQYTNVDRFATGSNLTFQIILYPSGRIVYQYL
                     SLSGVLDSATVGIQNQAKDDGLQVAFNAAYLHNNLAIEIKPIPAWLTASPTGGTLAAG
                     ASTTLTVDLNAAGLEDGLHEGRIDIASNDPYRPAITVPVSLMVGLVEPTYFNFDPDAI
                     NLAAMGTTIKVVIELPPAYDPHDIVIGSILLNDTVPAQPSPIAYSDENGNGIQELVVK
                     FNRQAVEATLGQGSSVPVTIQGEVLDTTWFRGTTTARVIRPRLTNPNSGGYFTQGMPV
                     TVTWEAAQGSGNIRHELWLSRDLGATWEPIAADLTGTSFLWTAGGTATDHGLIRVVAR
                     DSQGVMGYDTSDAAFTISAGPLMPPQPVGDTLELSADTADVILAWKRPAIDATHGPVA
                     LYRVLRAFSAQGPWEQVGTVATESLRDPLAGHGDATFVYYKVIAGNAAGDAAE"
     gene            2342..2848
                     /locus_tag="VFD06_01555"
     CDS             2342..2848
                     /locus_tag="VFD06_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02250.1"
                     /translation="MTTGLLLFTLFHVALSLIGIGAGLVVLCGLLASKRLDGWTVLFL
                     TTTIATSVTGFLFPVRGFTPAIGVGIISMIVLAVACYARYGRHLAGGWRLAYVVTAVI
                     ALYLNVFVLVVQAFLKIPALTALAPQQNEPPFAVAQGIVFVLFLVAGTRAAMRFRDVG
                     PAAAPQVA"
     gene            2865..3335
                     /locus_tag="VFD06_01560"
     CDS             2865..3335
                     /locus_tag="VFD06_01560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN02251.1"
                     /translation="MTIRGARPDERGLLLDLWLRSVRATHHFLTEADIQALLPLVRDQ
                     ALPALDLWVLCSPDGAVAGFMGLSGASVEALFLEPGFMRRGGGRRLLEHARRLKGRLS
                     VDVNEQNPEAVRFYEACGFEVVGRSPLDGGGRPFPLLHLREAASPRAPALDAKP"
     gene            3332..3781
                     /locus_tag="VFD06_01565"
     CDS             3332..3781
                     /locus_tag="VFD06_01565"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HZN02252.1"
                     /translation="MRLPAVSNRGWAIFFARAILGLIFFMAGTWKVFGLGPLEHARKW
                     FTGPYADSFLPMWSLWAVGTTIPFVELVAGALVIAGWRVREACVLLGAVLVIVTFGHL
                     LADPLYEFHTHVIPRTALLLFVLAMPREEDTLSIDGWLAARSEALRS"
     gene            complement(3778..4218)
                     /locus_tag="VFD06_01570"
     CDS             complement(3778..4218)
                     /locus_tag="VFD06_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN02253.1"
                     /translation="MQVRNHTSKKQNEHDVYVVNRYFELLHRKDFEQWQDLWNDLGRM
                     VFFYPPDGFPAILEGKAAIRAAVEAIFESFVSYTYDITALYPSPESGAVCVEYKVRAT
                     LTSNAEYCGSNILVFHFQEGLITSLHMYADPRAFQVIVDTRPKA"
     gene            complement(4341..4562)
                     /locus_tag="VFD06_01575"
     CDS             complement(4341..4562)
                     /locus_tag="VFD06_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02254.1"
                     /translation="MVLDEGTNEILVIRDPFRTRIEVHLKGETPDAYAMARLTPFEAR
                     RLAAILLFQAGKLDRPAAEKPRPTLRAIR"
     gene            complement(4660..6432)
                     /locus_tag="VFD06_01580"
     CDS             complement(4660..6432)
                     /locus_tag="VFD06_01580"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZN02255.1"
                     /translation="MNGASRIPGGIPAGTEGADLEFYRFADRYLADLFEAFPSAATAA
                     GYHRHDGRLDDWSPTGVRAKNTMARSHRASLLAVDARSLSPGARVDLGILRADTEATL
                     LSLEELRPHERDPQFFVDLLGNAILYLTLLDADSPAWPERLEGLRGRLLAVPDFLAGA
                     RATLDRPARVVTEMVAQTNAGNLRFLTDQLPRLFDKAPALAPELREANVRAIAALREF
                     QTFVEKDLLPRSTGDWRLGGALWDKKLHLTLQSEMDATTIAQRAERAIADLRRRMLEI
                     AEPLHARLFPGHAHGEAGDALVNLVVGEVIEAVSERRPTRTTLFETVRRAVDQIKGFL
                     RERDLISLPPEDDRLAIEPTPGFLDGMAVAFFNPPPTLEPGLKKSFWISSVPRGGTPE
                     EDARIEASFLREYNDYALHGLAIHEAFPGHYVQYWHAYRSPMATLYKKVFSSGTFAEG
                     WAVLAERLMFESGYNAEDPAYLLMHLKHGLRAPINALIDARLHTGDDSDEEVDRFALD
                     LLRRTGFQEETEARGKLRRAKVTSTQLSTYFVGLLEMQSLMDDAMRAAGPGANRKTVT
                     DRMLSFGTIPPRDVRSLMGLPPAR"
     gene            complement(6429..7169)
                     /gene="pgl"
                     /locus_tag="VFD06_01585"
     CDS             complement(6429..7169)
                     /gene="pgl"
                     /locus_tag="VFD06_01585"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZN02256.1"
                     /translation="MTQASPVRVLPDAAGVAREAAAEFARCAASACAARGAFQVAFSG
                     GTTPRTAYEALADPHGPHARGIPWDQVQVYFGDERQVPPDSEHSNARMACDALLRHVP
                     IPEGQIHRIRGENPDPDRAAEEYEEILRHRFRLAGGEWPRFDLVLLGMGADGHTASLF
                     PGSPALAETERLAVATRAPVEPIGRISLTLPVFNAAAAVIVLVTGAAKGAAFARVKSG
                     ADLPVARVRPADGSLLWLADRAAAGAAA"
     gene            complement(7166..8089)
                     /locus_tag="VFD06_01590"
     CDS             complement(7166..8089)
                     /locus_tag="VFD06_01590"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HZN02257.1"
                     /translation="MNDRVQEILSWYGSDSPGTLTNIARLLNHGRLAGTGKLVILPVD
                     QGFEHGPARSFAANPPAYDPRYHFELAVEAGCNAYAAPLGFIEGGARDYAGRIPLILK
                     INNKDSLLEEKDPTQAWTGSVHDALRLGCSAIGFTIYPGSSERFEMYERIRGLAEEAK
                     RSGLAVVIWSYPRGSGLDKDGETAIDVCGYAAQIAAQLGAHIIKVKLPTNYIAQDAAR
                     KVYEKEKIPIESLADRVKHVVQCAFAGRRIVIFSGGPAESDDKILGEVRGIRDGGGFG
                     SIIGRNSFQRKKADALKLLDNIMKIYSGQTP"
     gene            complement(8100..>8508)
                     /locus_tag="VFD06_01595"
     CDS             complement(8100..>8508)
                     /locus_tag="VFD06_01595"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF040139.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HZN02258.1"
                     /translation="ERGSTFSANLGRSREWHPHLRAYLDHLMASDKETKRPYSLRYIG
                     ALVADLHRCLIEGGIYFYPPDPKNTQGKLRLMYECLPLAFLTEQAGGRASDGTRRLLD
                     VEAASAHARSPLVLGSAKEVDLYETFMKTGRPA"
BASE COUNT         1193 a         2964 c         2995 g         1356 t
ORIGIN      
        1 cggtgtcgct gcacgtcacc ggcatcccgg acatcgacgc cgtgccggcg tcgctgacgt
       61 tcccgaccac cttcatcggc ttctcgtcgt cgctgccgct gagcctccgg aacccgggga
      121 ccgacgtgct gcacatcgcg agtctcgacg tcagcggcga ctactccgtc tccggcctga
      181 ccgcccccgc ggcgatcccg gccggcgggg ccatccccct cgtcgtcacc ttcacgccga
      241 ccggcgccgg tccgcggtcc ggggcggtcg ccatcgccag cgacgatccg gacgagtcgc
      301 cgttcgtcgt cagtctctcc ggcgcggcgc tcgtgccgcc ggccatcggc gtcgatccgg
      361 aggccatcgc gaccgcgctg ccgccggccg gtgcgcggac gaagagcctc accctctcga
      421 acaccggcgg gagcgatctc gtctggtcgc tcgggacggt gcggctcagc accacggtcc
      481 cgccgggcac ctacctggcg ctcggcaagg gggaggagga tccgcgcctg ggcatcctcg
      541 gaagcggcgg gccggacgcc ttcggctatg tcttcaagga ctccgacgag ccggacggac
      601 cggcgttcgc gtggaccgac atctcgggtg tcgggacacc gatcgccgcg ctcaccggcg
      661 acgaccagaa cgccgggccg atcgcgatcg gcttcccgtt cccgttctac ggcaacacgt
      721 tcacgagcgt ccgcgtcgcg accaacggct ggctgtcgtt cacctcgacc tccaccgcac
      781 acatcaacca gccgctgccg aacaccgcgt ccgcggtgcc ggagaacctg gtggcgggcc
      841 tctgggacga cctgcacttc cgcggcgcgg tccgggcgca ctcgtacaac gacgggtcgc
      901 ggttcatcgt ccagtacacc aacgtcgatc gcttcgcgac cgggtcgaac ctgaccttcc
      961 agatcatcct ctacccgagc ggccggatcg tgtaccagta cctgtcgctc tccggggtcc
     1021 tcgactccgc gaccgtcggg atccagaacc aggcgaagga cgacggcctc caggtggcct
     1081 tcaacgcggc ctacctgcac aacaacctgg cgatcgagat caagccgatc ccggcgtggc
     1141 tgacggcgtc gccgactggc ggcacgctgg ccgccggcgc ctcgacgacc ctcaccgtcg
     1201 acctcaacgc ggccgggctc gaagacggcc tgcacgaagg gcgcatcgac atcgcgagca
     1261 acgacccgta ccggcccgcg atcacggtgc cggtgtcgct catggtcggg ctggtcgagc
     1321 cgacctactt caacttcgat ccggacgcga tcaacctcgc ggcgatgggg accacgatca
     1381 aggtggtgat cgagctgcct ccggcctacg acccgcacga catcgtcatc gggtcgatcc
     1441 tgttgaacga cacggtgccg gcgcagccgt cgccgatcgc gtactcggac gagaacggca
     1501 acggcatcca ggagctggtg gtcaagttca accgccaggc ggtggaggcg acgctgggcc
     1561 agggctcgtc ggtgccggtg acgatccagg gggaggtcct cgacaccacc tggttccgcg
     1621 gcaccaccac ggcgcgggtc atccggccgc ggttgacgaa ccccaactcc ggcggctact
     1681 tcacgcaggg gatgccggtg accgtcacct gggaggcggc gcagggctcg gggaacatcc
     1741 ggcacgagct ctggctcagc cgcgacctcg gcgccacctg ggagccgatc gccgcggacc
     1801 tcaccgggac gtcgttcctc tggaccgccg gaggcacggc caccgaccac gggttgattc
     1861 gggtcgtggc ccgcgacagc cagggtgtga tggggtacga cacgagcgac gccgcgttca
     1921 ctatctccgc ggggccgctc atgccgccgc agccggtcgg tgacacgctc gagctgagcg
     1981 ccgacacggc cgacgtcatc ctggcctgga agcgtccggc gatcgacgcg acgcacgggc
     2041 cggtggcgct gtaccgggtc ctgcgtgcct tctcggcgca ggggccgtgg gagcaagtgg
     2101 gcacggtcgc gaccgagtcg ctgcgtgacc cgctggccgg ccacggcgac gccacgttcg
     2161 tctactacaa ggtgatcgcc ggcaacgccg ccggcgacgc cgcagagtaa cgcggagtaa
     2221 ggggaagctc cggccgcttc cgggtggcac cgacgccgac ggtgcccacc cgggggcggc
     2281 cggatggtcc gcaggcgtat gatccggccg ctctcgtcgc actgctgcgg aggaacggcc
     2341 gatgacgacc ggtttgcttc tgttcacgct gttccacgtc gcgctgagcc tcatcgggat
     2401 cggcgcaggg ctcgtggtcc tgtgcgggct gctcgcctcg aagcggctgg atggctggac
     2461 cgtcctcttc ctgaccacca cgatcgcgac cagcgtgacc gggtttctct tcccggtccg
     2521 cgggttcaca ccggcgatcg gcgtcggcat catttcgatg atcgtcctgg ccgtcgcctg
     2581 ctacgcgcgc tacggcaggc acctcgccgg cggctggagg ctggcgtacg tggtgacggc
     2641 ggtcatcgcc ctttatctca acgtcttcgt gctggtggtt caggccttcc tgaagatccc
     2701 ggcgctcacc gcgctggcgc cgcagcagaa cgagccgccg ttcgccgtcg cgcaggggat
     2761 cgtcttcgtc ctgttcctcg tcgccggcac ccgcgccgcg atgcgcttcc gcgacgtcgg
     2821 gccggccgcc gcccctcaag ttgcctgagc gcggcatcgg cgccatgacc atccggggcg
     2881 cacggcccga cgagcgcggc ctgctgctcg acctctggct gcgttcggtg cgcgccaccc
     2941 accatttcct gaccgaggcc gacatccagg cgctcctgcc gctggtgcgc gaccaggcgc
     3001 tcccggcgct cgacctgtgg gtcctgtgct cgcccgacgg cgccgtcgcc gggttcatgg
     3061 ggctctccgg agcaagcgtc gaggcgttgt tcctcgaacc cgggttcatg cgccggggcg
     3121 gaggccggcg cctcctcgaa catgcgcgcc ggctgaaagg gcggctctcc gtggatgtca
     3181 acgagcagaa tccggaggcg gtccgcttct acgaggcgtg cggcttcgag gtcgtcggcc
     3241 gatccccgct cgacggtggc ggtcggccgt ttccactgct gcacctccgc gaggcggcgt
     3301 cgccgcgggc gcccgcgctg gacgcgaagc cgtgaggctg ccggccgtca gcaaccgcgg
     3361 ctgggcgatc ttcttcgcgc gcgccatcct cggcctcatc ttcttcatgg ccgggacgtg
     3421 gaaggtcttc ggcctcggcc cgctggagca cgcgcggaag tggttcaccg gtccctacgc
     3481 cgactcattt ctcccgatgt ggtcgctgtg ggccgtcggg acgacgatcc ccttcgtcga
     3541 gctggtcgcg ggggcgctgg tcatcgccgg gtggcgggtg cgcgaggcgt gcgtcctcct
     3601 cggggcggtc ctcgtgatcg tcaccttcgg ccacctgctc gcggatccgc tctacgagtt
     3661 ccacacgcac gtcatcccgc gcacggcgct gctcctgttc gtgctcgcca tgccgcgcga
     3721 ggaggacacg ctgtcgatcg acgggtggct cgccgcccgg agcgaggccc tgcgctccta
     3781 ggccttcggc cgggtgtcga cgatgacctg gaaggcgcgc gggtcggcgt acatgtgcag
     3841 gctggtgatg agtccctcct ggaagtggaa caccaggatg ttgctgccgc agtactcggc
     3901 gttgcttgtc agcgtcgcgc ggaccttgta ctcgacgcat accgcccccg actcgggcga
     3961 gggatagagc gcggtgatgt cgtaggtgta ggagacgaac gattcgaaga tcgcttcgac
     4021 ggcggcacgg atggccgcct tcccttcgag gatcgccggg aacccgtcgg gcgggtagaa
     4081 aaagaccatc cgcccgaggt cattccagag atcctgccac tgctcgaaat ccttgcggtg
     4141 cagcagctcg aagtagcggt tgacgacgta gacgtcgtgc tcgttctgct ttttcgacgt
     4201 gtgatttcgg acctgcatgg ggcaattatt ctgcaggttc cggcgacgct tttcgagtac
     4261 ccgatactac gcagacggcg tcgggctcaa tccggccgaa gaacactccg ccggggaatc
     4321 ttcccgatga ccgtcgattt tcagcgaatc gcccggagcg tgggccgcgg cttctcggct
     4381 gcagggcggt cgagcttccc cgcctgaaac agcaggatcg ccgcgaggcg gcgcgcctcg
     4441 aatggcgtca ggcgggccat cgcgtaggcg tcgggcgtct cgcccttcag gtgcacctcg
     4501 atccgggtgc gaaaggggtc ccggatgacg aggatctcgt tcgtgccttc gtcgagcacc
     4561 atgttcggtc ctcctttccc gtgtcagcat ggcgtagcgg gatgaggtcc gcgtgacgga
     4621 aacggctcga tcgcgtgaag tcagggggag tgaagagggt cagcgagccg gcggcagccc
     4681 catcagcgac cggacgtccc ggggggggat ggtcccgaac gacagcatgc ggtcggtgac
     4741 cgtcttccga ttcgctccgg gtccggcggc gcgcatcgcg tcgtccatca ggctctgcat
     4801 ctcgagaagc ccgacgaagt aggtcgagag ctgcgtcgag gtcaccttgg cgcggcgcag
     4861 cttgccgcgc gcctcggtct cctcctggaa accggtgcgg cgcaggaggt ccagcgcgaa
     4921 acggtcgacc tcctcgtcgg aatcgtcgcc ggtgtggagg cgggcgtcga tgagggcgtt
     4981 gatcggggcg cgcagcccgt gcttgaggtg catcaggaga tacgccgggt cctcggcgtt
     5041 gtagccgctc tcgaacatca gccgctcggc gaggaccgcc cagccttcgg cgaaggtccc
     5101 cgaggagaag accttcttgt acagggtggc catgggggag cggtacgcgt gccagtactg
     5161 cacgtaatgc cccgggaacg cctcgtggat cgccaggccg tggagcgcgt agtcgttgta
     5221 ttcgcgcagg aaggaggcct cgatgcgggc gtcctcctcg ggggtgccgc cgcgcggcac
     5281 cgaggagatc cagaacgact tcttgagccc cggctcgagg gtggggggcg ggttgaagaa
     5341 cgcgaccgcc atcccgtcga ggaacccggg ggtcggctcg atcgccagcc ggtcgtcctc
     5401 gggcggcagg ctgatgaggt cccgctcccg caggaatccc ttgatctgat cgacggcgcg
     5461 ccgcaccgtc tcgaagagcg tggtccgtgt cgggcggcgt tccgagaccg cctcgatcac
     5521 ctccccgacg acgaggttca cgagcgcgtc cccggcctcg ccgtgcgcgt gcccggggaa
     5581 gaggcgcgcg tgcagaggct cggcgatctc cagcatgcgg cggcgcaggt cggcgatcgc
     5641 ccgctccgcc cgctgcgcga tggtcgtcgc gtccatctcc gactgcaggg tcaggtggag
     5701 tttcttgtcc cacagcgccc cgccgagccg ccaatccccg gtggagcggg gcaggaggtc
     5761 cttctcgacg aaggtctgga attcgcgcag ggccgcgatg gcgcggacgt tcgcttcgcg
     5821 cagctccggg gccagcgccg gggccttgtc gaagaggcgc ggcagctggt cggtcaggaa
     5881 gcgcaggttc ccggcgttgg tctgcgcgac catctcggtg accaccctgg cgggccggtc
     5941 gagcgtcgcg cgcgcgccgg cgaggaagtc cggcaccgcc agaagccgcc cgcgcagccc
     6001 ctccagccgc tccggccagg cgggggagtc ggcgtcgagg agggtcaggt agaggatcgc
     6061 gttgccgagg aggtcgacga agaactgcgg gtcgcgctcg tgcgggcgca gttcctccag
     6121 cgacaggagg gtcgcctcgg tgtcggcgcg caggatcccc agatcgacgc gcgcccccgg
     6181 gctgaggctc cgcgcgtcga cggccagaag ggaagcgcgg tgcgagcgcg ccatggtgtt
     6241 cttcgcccgg accccggtgg ggctccagtc gtcgaggcgg ccatcgtgcc ggtggtaccc
     6301 ggccgccgtg gccgccgacg ggaacgcctc gaagagatcg gcgaggtagc gatcggcgaa
     6361 gcggtagaac tcgagatccg ccccttcggt ccccgcgggg atcccgcccg ggatccgcga
     6421 ggcgccgttc atgccgcggc ccctgccgcc gcccggtcgg cgagccacag aagcgagccg
     6481 tccgccggcc ggacgcgcgc caccggcagg tcggcgccgc tcttcacgcg cgcgaacgcc
     6541 gcccccttcg cggcgccggt gaccaggacg atgaccgccg ccgccgcgtt gaagaccggc
     6601 agcgtcaggc tgatgcggcc gatcggctcg accggcgccc gggtcgcgac cgcgaggcgc
     6661 tcggtctcgg cgagcgccgg cgatccgggg aagagcgacg cggtgtggcc gtccgccccc
     6721 atgccgagga gcaccaggtc gaaacgcggc cactcccccc cggcgaggcg gaaccggtgc
     6781 cgcaggatct cctcgtattc ctccgcggcc cgatccgggt ccgggttctc gcctcggatg
     6841 cgatggatct gcccctccgg gatcggcacg tggcgcagga gcgcgtcgca ggccatgcgg
     6901 gcgttgctgt gctccgaatc tggggggacc tgccgctcgt cgccgaagta gacctgcacc
     6961 tggtcccagg ggatcccccg cgcatgcggc ccatgtgggt cggcgagcgc ctcgtaggcg
     7021 gtgcgcggcg tcgtcccccc ggagaaggcc acctggaagg cgccgcgcgc ggcgcaggcg
     7081 gaggccgcgc agcgcgcgaa ctcggccgcg gcctcgcgcg ccactcccgc cgcgtccggc
     7141 aggacccgga ccggagaggc ctgcgtcagg gagtctgccc cgagtagatc ttcatgatgt
     7201 tgtccagaag cttgagcgcg tccgccttct tccgctggaa cgagttgcgg ccgatgatcg
     7261 acccgaaccc gccgccatcg cggatgccgc gcacctctcc gaggatcttg tcgtcggact
     7321 cggccgggcc gcccgagaag atgacgatgc ggcggccggc gaaggcgcac tgcacgacgt
     7381 gcttcacgcg gtccgccagg ctctcgatcg ggatcttctc cttctcgtag actttccgcg
     7441 cggcgtcctg ggcgatgtaa ttcgtcggca gcttcacctt gatgatgtgg gccccgagct
     7501 gcgccgcgat ctgcgccgcg tagccgcaca cgtcgatggc ggtttcgccg tccttgtcga
     7561 ggcccgagcc gcgcggatac gaccagatga cgaccgcgag ccccgagcgc ttggcctcct
     7621 ccgccaggcc gcggatccgc tcgtacatct cgaagcgctc cgacgatccc gggtagatgg
     7681 tgaaaccgat ggcgctgcaa ccaaggcgca gcgcgtcgtg caccgagccg gtccaggcct
     7741 gcgtcgggtc cttctcctcg aggagcgagt ccttgttgtt gatcttcaga atgagcggga
     7801 tgcggccggc gtagtcgcgc gcccccccct cgatgaaccc gagcggcgcc gcgtaggcgt
     7861 tgcagccggc ctcgaccgcc agctcgaagt ggtagcgcgg gtcgtaggct ggcgggttgg
     7921 cggcgaacga gcgggccggg ccgtgctcga atccctggtc caccggcagg atgacgagct
     7981 tcccggtgcc ggcgaggcgg ccgtggttca ggagccgggc gatgttggtc agcgttccgg
     8041 ggctgtcgct cccgtaccac gaaaggatct cctgcacgcg atcgttcacg gtcgtctcct
     8101 caggccggcc ggccggtctt catgaaggtt tcgtacagat cgacttcctt cgcgctgccg
     8161 agaacgagcg gcgagcgcgc gtgcgccgag gccgcctcga cgtcgagcag ccgccgcgtc
     8221 ccgtcgctgg cgcgccctcc cgcctgctcg gtcaggaagg cgagcggcag gcactcgtac
     8281 atcaggcgca gcttcccctg cgtgttcttc ggatcggggg gatagaaata aatccctccc
     8341 tcgatgaggc agcgatgcag gtcggccacc agcgccccga tgtagcgcag ggaatagggt
     8401 cgcttggtct ccttgtcgct cgccatgagg tgatcgaggt aggcgcgcag gtgcgggtgc
     8461 cattcgcgcg accgcccgag gttggcgctg aacgtcgagc cgcgctcg
//
LOCUS       DASRIQ010000061         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2634042, whole genome shotgun sequence.
ACCESSION   DASRIQ010000061 DASRIQ010000000
VERSION     DASRIQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634042"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFD06_01600"
     CDS             <1..533
                     /locus_tag="VFD06_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02259.1"
                     /translation="AYGVSSNSVVAPADGPETTSLGRFLDAARAAGGATHRFRVMQWP
                     MNLFEGGAALERNTGPGGEQTALETARDADLKVLVNRPLNAILDGRLVRLSDASPAAR
                     AVAEHLDPLLPEHLRSVPLSQKAIAVLLSTPGIGTVLVGMRRPAYVDDVLAAAALPAV
                     PDPLPIYRRFSELTGG"
     gene            complement(558..1019)
                     /locus_tag="VFD06_01605"
     CDS             complement(558..1019)
                     /locus_tag="VFD06_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02260.1"
                     /translation="MRPPPARGPRLAQRLAAAALGAALCAGCSDTDLNPLPTVGSDDG
                     DPVVAQPSFARDVQPIFTARCAVAGCHITPTEANLGLVLTDAATSYAHLVNVPSGEVT
                     GGVLRVLPGSAIMSWLMVKLDTGEMPKEGAMLSQGTRDTIRNWIDQGAQNN"
     gene            complement(1016..1891)
                     /locus_tag="VFD06_01610"
     CDS             complement(1016..1891)
                     /locus_tag="VFD06_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02261.1"
                     /translation="MEQPTRTMGRTAAPAALAALAWLGASGAVLAAVPVEDDSNEAQL
                     MGYYAAALSFTPLGAPAGRLVEAAIDVTYLPSLSEEERQTTFAGAKLENTNFTSVIPR
                     PRIRWRPDEDWLLEAGYVPPVELFGVTPQMLGAAAAWRFAAPAEAPAWWIRAHYMRAD
                     IEGPITCPEEAVEDPENSVCFGGQVSEDRFRPETYGLDVLVSGAHLWTGGPAWYAVAG
                     YRHERLQFNTHFVNSFGRLDDQVLVARLDRGSIGGGVSWDAWRGLRLNAEAWYAPEAL
                     FTGRVAIAWGWGETR"
     gene            1943..2512
                     /locus_tag="VFD06_01615"
     CDS             1943..2512
                     /locus_tag="VFD06_01615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZN02262.1"
                     /translation="MRFVRIVTFCGSIRSGSLNRKLLAQAIEVLRPSAEIDAAELRDV
                     PLPIYDGDLETSSGVPDPAKRLAGRIAAADGLVIASPEYNNMIPGGLKNAIDWMSRLK
                     PQPFKGKPVLLLGASPGAYGAVRSQLALRQVITSLFAILVPNGVTLPHADQAFEEDGR
                     LKDPKVRAQIERACAELLRFIAAFPAQRA"
     gene            2521..>3056
                     /locus_tag="VFD06_01620"
     CDS             2521..>3056
                     /locus_tag="VFD06_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN02263.1"
                     /translation="MRAAVMPAPGAPIEVRDLPVPALEPGAVLLETIASEVCGTDVHL
                     WRGRLAGVPYPIIPGHVAVGRIAAIGSADRAPASGSSATAPADLEGRRFEVGEVVTFV
                     DVHGTCGACWYCLVARQSTRCPKRRVYGITMGVADGPSGGWAERILLRPGTQLLRLPP
                     GLEPATFIGGGCGAVTAF"
BASE COUNT          411 a         1136 c         1073 g          436 t
ORIGIN      
        1 gcgcctacgg cgtctcctcg aacagcgtgg tggcgcccgc cgacggcccg gagacgacct
       61 cgctcggccg tttcctcgat gcggcgcgcg cggccggcgg cgcgacgcat cgcttccgtg
      121 tgatgcaatg gccgatgaac ctgttcgaag gcggggcggc gctcgagcgg aacaccgggc
      181 cgggcgggga acagaccgcg ctcgagacgg cgcgagacgc ggacctcaag gttttggtca
      241 accggccgct caacgccatt ctcgacggga ggctcgtgcg gctctcggac gcctcgcccg
      301 ccgcgcgcgc ggtcgcggag cacctcgacc cgctgctccc ggagcacctc cgatccgtcc
      361 cgctgtcgca gaaggcgatc gcggtcctgc tctcgacacc ggggatcgga acggtcctcg
      421 tggggatgcg gaggcccgcc tacgtcgatg acgtcctcgc ggcggcggcg cttcccgccg
      481 ttccggaccc gctccccatc taccgcaggt tctccgagct gaccggcggc tgaagcggcg
      541 gcggtcgcgt cccggcctca gttgttctgg gccccctggt cgatccagtt gcgaatggtg
      601 tcgcgcgtcc cctgcgacag catcgcccct tccttcggca tctcgccggt gtcgagcttg
      661 accatcagcc acgacatgat cgccgacccc ggcaggacgc gcaggacgcc gccggtgacc
      721 tcccccgacg gcacgttcac gaggtgggcg taggaggtcg cggcgtccgt caggacgaga
      781 ccgagattcg cctcggtcgg tgtgatgtgg cagccggcga cggcgcagcg ggccgtgaag
      841 atcggctgga cgtcgcgggc gaacgacggc tgcgccacga ccggatcgcc gtcgtcgctg
      901 ccgacagtcg gcagcggatt gaggtcggtg tcgctgcacc cggcacacag cgcggcgccg
      961 agcgccgccg ccgcgagccg ctgcgcgagc cgggggcctc tggccggcgg aggtctcatc
     1021 gtgtctctcc ccatccccag gcgatcgcca cgcgcccggt gaagagtgcc tccggcgcgt
     1081 accaggcctc ggcgttgaga cgcagcccgc gccaggcatc ccagctgaca ccgccgccga
     1141 tcgacccgcg atcgagccgg gcgacgagga cctgatcgtc gagccgtccg aagctgttca
     1201 cgaagtgcgt gttgaactgc agccgctcgt gccggtagcc ggcgaccgcg taccaggccg
     1261 gcccgccggt ccaaagatgt gcgcccgaga ccagcacgtc gaggccgtag gtctccggac
     1321 ggaagcgatc ctcgctgacc tgaccgccga agcagacgct gttctccgga tcctcgaccg
     1381 cctcctccgg gcaggtgatc ggcccttcga tgtcggcccg catgtagtgc gcccggatcc
     1441 accatgccgg tgcctccgcc ggggcggcga agcgccaggc cgcggccgcc ccgagcatct
     1501 gaggcgtcac gccgaacagc tcgaccggag ggacgtagcc ggcctcgagt agccagtcct
     1561 cgtccggacg ccagcggatc cgcggccgcg ggatgacgga ggtgaagttg gtgttctcga
     1621 gcttcgcgcc ggcgaaggtg gtctggcgtt cctcttcgct gagcgaggga aggtacgtca
     1681 cgtcgatggc cgcctcgacg agccgcccgg ccggggcgcc gagcggcgtg aacgacagcg
     1741 ccgccgcgta gtaccccatg agctgcgcct cgttcgagtc gtcctcgacc ggcaccgcgg
     1801 cgagcaccgc cccggacgcg ccgagccagg ccagcgccgc aagtgccgcc ggcgccgccg
     1861 tccggcccat cgtccgcgtc ggctgttcca tggtccgcgg agcgtagcac cgcccccccg
     1921 cacgccggac cgccgatgta agatgcgttt cgtgcgtatc gtcacattct gcggctcgat
     1981 ccggagcggc tcgctgaacc ggaagctcct ggctcaggcg atcgaagtgc tccgcccctc
     2041 ggccgagatc gacgcggcgg aactgcgcga cgtgccgctg ccgatctacg acggggacct
     2101 cgagacatcg agcggcgtcc cggacccggc gaagcgcctg gccgggcgga tcgccgcggc
     2161 cgacgggctg gtgatcgcct cccccgagta caacaacatg attcccggcg gcctcaagaa
     2221 cgccatcgac tggatgtcgc gcctcaagcc gcagccgttc aaggggaagc cggtgctgct
     2281 cctcggcgcc tcccccgggg cctacggcgc cgtccggtcg cagctcgcgc tccgccaggt
     2341 gatcacctct ctcttcgcca tcctcgtgcc gaacggcgtg accctgccgc acgctgacca
     2401 ggcgttcgaa gaagacggcc gcctgaaaga cccgaaagtc cgcgcgcaga tcgagcgcgc
     2461 ctgcgccgag ctgttgcgct tcatcgccgc cttcccggcg cagcgcgcct gagggcggcg
     2521 atgcgtgccg cggtgatgcc ggcccccggc gcgccgatcg aggtgcgcga tctcccggtc
     2581 cccgcgctcg agccgggggc ggtcctcctc gagacgatcg cctccgaagt gtgcggcacc
     2641 gacgtgcacc tctggcgcgg ccggctcgcc ggcgtcccct acccgatcat cccggggcac
     2701 gtcgcggtcg ggcgcatcgc cgcgatcgga tccgcggacc gggcgccagc ctccggcagt
     2761 tccgcgaccg cgcccgcgga tctcgagggg cgtcgcttcg aagtggggga ggtggtcacc
     2821 ttcgtggatg ttcacggcac gtgcggggcg tgctggtact gcctcgtggc gcgccagtcg
     2881 acccgctgcc cgaagcggcg cgtctacggc atcaccatgg gagtggcgga cgggccgagc
     2941 ggcggctggg ccgagcggat cctgctgcgc cccggcacgc agctcctgcg cctgccgccc
     3001 gggctcgaac ctgcgacctt catcggcggc ggctgcggcg cggtgaccgc cttcca
//
LOCUS       DASRIQ010000062         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_279052, whole genome shotgun sequence.
ACCESSION   DASRIQ010000062 DASRIQ010000000
VERSION     DASRIQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279052"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFD06_01625"
     CDS             complement(<1..518)
                     /locus_tag="VFD06_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZN02264.1"
                     /translation="MPRATDRRVLLAVGAHPDDVEFGCGAILIKEADRGHAVTVLNLS
                     RGEAGTSGTPEVRSRESAAAAQRMGASLEWLELDGDARLEERADNAFAIARAIRRIRP
                     NLVLAPLPVENQHPDHGRAGRMVRDAARLARYGGLKGLETSAPHAIDALYFYDITGAG
                     GGTGPADPSLAR"
     gene            complement(502..1302)
                     /locus_tag="VFD06_01630"
     CDS             complement(502..1302)
                     /locus_tag="VFD06_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02265.1"
                     /translation="MNEPVICFGQQPCGFFPKRFLYAKLTAARRLRGEIGGRIVWFFH
                     DSDHDYRETTTPLRDRQTGELKRLNFDHTGKIQKKYSPLYAKRILPGWRDRTARVLPI
                     FVEPALVDAFEQVGAETAAGFCLEMYRRLGLLEGVEIVRSGDPEVRRRAIDIDDCYLD
                     VPYEGEIVRARRQGDRYILHRGGDQFLEIPGPERVGKEQVSPTRDSRLRWMQSVVRCT
                     HYVCGAGEAQYLKRDEAPEIAFVERDVIDRPHESWIGDEEEGRDAARH"
     gene            1488..2516
                     /locus_tag="VFD06_01635"
     CDS             1488..2516
                     /locus_tag="VFD06_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZN02266.1"
                     /translation="MRAMILDRIAPLSERPAPLTAVTWPDPAPGPGEIVLHVSACGVC
                     HTELDEIEGRLPPPRLPVIPGHQAVGRVESLGDGARRFAVGDRVGASWIHSACGSCAY
                     CRDGRENLCPDFRATGRDAHGGYAERMSIAEAFAIPIPDGLDDLHAAPLLCAGAIGWR
                     SLRLSRHAPGRALGLTGFGASAHLVLRTVLATDPGAEVFVFARDAEQRGFALRLGAAW
                     AGDTLDRAPRRLDAVIDTTPAWTPVLGALQSLAPGGRLVINAIRKEAGDQEALLRLDY
                     PEHLWMEKEITSVANVTRADVTEFLALAARAGLRPDVRVYPLEEANRALGDLKKGGGS
                     GALVLRLV"
     gene            complement(2535..2756)
                     /locus_tag="VFD06_01640"
     CDS             complement(2535..2756)
                     /locus_tag="VFD06_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02267.1"
                     /translation="MSGLEQEFPGKVKAENVDASTEEAKKAIKDLGFKNHGLVIRSAK
                     GEVLHKQPDHSVDIDAARKAIQEILAKGA"
BASE COUNT          384 a         1048 c         1044 g          462 t
ORIGIN      
        1 atccgtgcca gcgagggatc cgccggcccg gtgcccccgc cggcgccggt gatgtcgtag
       61 aagtagagcg cgtcgatggc gtgcggggca gaggtctcga ggcccttgag gccaccatag
      121 cgggccaggc gcgccgcgtc gcgcaccatc cgcccggcgc ggccgtgatc cgggtgctgg
      181 ttctcgaccg gcagcggcgc gagcaccagg ttcgggcgga tccggcggat ggcgcgggcg
      241 atggcgaagg cgttgtcggc ccgctcctcg aggcgggcgt cgccgtcgag ctcgagccac
      301 tcgaggctcg ctcccatgcg ctgcgcggcc gcggccgact cgcgcgaacg gacctccggc
      361 gtgccgctcg tccccgcctc gccgcgcgac aggttgagca ccgtcaccgc gtggccgcgg
      421 tccgcctctt tgatcaggat ggcgccgcag ccgaactcga cgtcatccgg gtgggccccg
      481 accgccagca ggacgcgccg atcagtggcg cgcggcatcg cgtccctcct cctcgtcgcc
      541 gatccacgac tcgtgcgggc ggtcgatgac gtcccgctcg acgaaggcga tctccggcgc
      601 ctcgtcgcgc ttcaggtact gcgcctcgcc ggcgccacag acgtagtgcg tgcagcgcac
      661 gaccgactgc atccagcgca ggcgcgagtc gcgcgtcggg ctcacctgct cctttccaac
      721 ccgctccggg ccggggatct cgaggaactg atcgccgccg cggtgcagga tgtaccggtc
      781 cccctgccgc cgggcccgga cgatctcccc ctcgtagggg acgtccaggt agcagtcgtc
      841 gatgtcgatc gcccggcgcc gcacctccgg atcgccggag cggacgatct ccacgccctc
      901 caggagcccg aggcgccggt acatctccag gcagaaaccg gccgccgtct ccgcgccgac
      961 ctgctcgaag gcatcgacga gcgccggctc gacgaagatc gggaggacgc gcgccgtgcg
     1021 gtcccgccag ccggggagga tgcgtttggc gtagagcggg gaatacttct tctggatctt
     1081 gccggtgtgg tcgaagttga gccgcttcag ctcgccggtc tgccggtcgc gcagcggcgt
     1141 ggtcgtctcg cggtaatcgt ggtcgctgtc gtggaagaac cagacgatgc ggccgccgat
     1201 ctccccccgc aggcggcgcg cggcggtgag cttggcgtac aggaagcgct tggggaagaa
     1261 gccgcagggc tgctgcccga agcagatgac cggctcgttc atgtcgaaat ccgggatccg
     1321 gacggatcga tggtcgcgcg tgttcctgag gccccccgag cccgcgaacc accttcgtgg
     1381 accccgtcga gctggccggc ggagagggta tcaccggtgc gcggtttcgg cgaagggcgg
     1441 gacggcgggg ccgatggtat ggtggccgtg gcagacaagg gccgacgatg cgcgccatga
     1501 tcctcgaccg gatcgcgccg ctgagcgaac gacctgcgcc gctcaccgcg gtgacctggc
     1561 cggacccggc tccggggccg ggcgaaatcg tcctccatgt gtccgcctgc ggcgtttgtc
     1621 acaccgagct cgacgagatt gaaggacggc tgcctccgcc gcgcctgccg gtgatccccg
     1681 ggcaccaggc ggtcgggcgg gtggagtcgc tcggcgacgg ggcgcggcga ttcgccgtcg
     1741 gcgaccgcgt cggtgcgtcg tggatccact ccgcctgcgg ttcctgcgcg tattgccgcg
     1801 atggccgcga gaatctctgc ccggatttcc gcgccaccgg ccgcgacgcg cacggtggtt
     1861 acgccgagcg aatgagcatt gccgaggcgt tcgcgatccc gatcccggat gggctcgacg
     1921 atctgcacgc ggcgccgctc ctctgcgccg gggcgatcgg ctggcgctcg ctgaggctct
     1981 ccaggcacgc gccgggacgg gcgctcggcc tgaccggctt cggcgcctcg gcgcacctgg
     2041 tgctgcgcac ggtgctcgcc acggatcctg gcgcggaggt gttcgtcttc gcgcgcgacg
     2101 cggagcagcg cggcttcgcg ctgcggctcg gggcggcctg ggccggcgac acgctcgacc
     2161 gggcgccgcg gcgcctcgac gccgtcatcg acaccacccc ggcctggact cctgtcctcg
     2221 gcgccctgca gagcctggcg ccggggggac gcctggtgat caacgccatc cgcaaggagg
     2281 cgggtgatca ggaggcgctc ctgcgcctcg actacccgga gcacctctgg atggagaagg
     2341 agatcacttc ggtggcgaac gtcacgcgcg ccgacgtcac ggagttcctg gcgctcgcgg
     2401 cccgcgccgg cctgcgcccg gacgtccgcg tgtacccgct cgaggaggcg aaccgggccc
     2461 tcggcgatct gaagaagggg ggcggctccg gcgccctcgt gctccggctc gtctgacccg
     2521 cgtccctctc gcgatcaggc tcccttcgcg aggatctcct ggatcgcctt gcgcgccgca
     2581 tcgatgtcga ccgagtggtc cggctgcttg tgcaggacct cccccttcgc ggatcggatc
     2641 acgagaccat gattcttgaa cccgaggtcc ttgatcgcct tcttcgcctc ctcggtcgac
     2701 gcgtccacgt tctccgcctt caccttgccc gggaactcct gctcgagccc gctcacggcg
     2761 ggcttcagct tcgcgcagac cattcagccg gggaggaagt agtaggtgac cagcagtggc
     2821 ccgtccgaag gagctttcgg agcgttcgcg ttcgcgatcg gcgacagcac cgtcatggcg
     2881 cacgcgagcc agatcaatgc ggaagcgatt ctcgtcttca tcgtggtgtt tcctcctc
//
LOCUS       DASRIQ010000063         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2634425, whole genome shotgun sequence.
ACCESSION   DASRIQ010000063 DASRIQ010000000
VERSION     DASRIQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634425"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..736
                     /locus_tag="VFD06_01645"
     CDS             68..736
                     /locus_tag="VFD06_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN02268.1"
                     /translation="MSGRENGGDVLTQAECGDPALGARARDYVLGTLEPDSAERMEDH
                     LAGCVACSARVTRYEEGLRRLQVDARGPLRAVRLPLLALAAILFVASALVLWHETRGL
                     APGDGPWRSPGPSFTLLSPAGAVATPPSELRWSAPDPQADCRAEIVRLDLAPLFEERA
                     AAPGRIPLPEGTRAALASGDEFLWRVRCSGASGEETSSYVGFQVVTSPAPGSDGATRE
                     SPPR"
     gene            complement(681..>3253)
                     /locus_tag="VFD06_01650"
     CDS             complement(681..>3253)
                     /locus_tag="VFD06_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZN02269.1"
                     /translation="RAQDDQVLAAVLNSLGILHRRRGELDRALAITVEGLELAQRHGD
                     LKGQAELVGSLAHIHSTAGDLAAAERAVRTALALAEKAQAPPGLSAVLLSNLASTLSQ
                     RGEVEQAMRTLDRIQRLPPGEGVQNYGPFITEALLSRGLGELDRAEALARRGLEVAQA
                     AGNREDALACEYDLAIILADRRRFDEAAERLERLVGDFDRGDRTREALYAINSLAALN
                     IERGDLDAAGRWNRLALVRLPGRPGGYPDAAGEAHSNRSRIAWARGDRDEAIRAADEQ
                     VEAERAFDRMHYVAGALEWRGELFEAAGRDEEAERDWREAMALRSQIRARILPTESIL
                     SLDRRRADAWRRLALLQLRRAAADPDSAAGRRDDAFRLLDDSRARRLVEALSRPRPVA
                     PVLSDGEGARAAHAAISALQSELLRGAPDAPTRARIEADLLLAEDRLRRAARDEAEAL
                     RRPPPASPAPALDLTALGGALLETRTALVTFLLGPGGGYALLHDGTAIRSLPLGHWEE
                     IDAMALRLEPLLRRPPDSTDAPPSAPALESLARSLVDPWIGMLPEGTRRLLIVPDGAL
                     ERVPFEALPLRGAGGATLGGRYVTAYLPSAATWLALRSRPSGSPTGRVLALAPDPGPG
                     FAPLPEGRREAERWAARGGWGSRCITGGIGAGETALLAIDLTGFDVLHIAAHGVADTA
                     HPLRSGLVLGPGGPVEDDLLQMREIPSLRLNAPLVVLAACRSGVGPTDRAEGVIALPT
                     AFLEAGARGAVATLWEVPDGVAARFMSRFLAALGRSGSPAESLQAVRSEFAASGDARL
                     RHPGAWAPFVYVGDPDAGVRLPWIGAGLARLLILGAALLVVTAAAILWSRRRSPAPAR
                     "
BASE COUNT          416 a         1225 c         1222 g          390 t
ORIGIN      
        1 tgtaccacgc gcgcatcaag atcggcctgg ccttgaatcg ccgggccgtc gggaggcaca
       61 ggtcttcgtg agcgggaggg aaaacggcgg cgatgtgctg acgcaggcgg agtgcggcga
      121 tccggccctc ggcgcgaggg cccgggacta tgtcctcggc acgctcgagc ctgattccgc
      181 cgaacggatg gaggaccacc tcgccggctg cgtcgcctgc tcggcccgcg tgacacggta
      241 cgaggagggc ctccggagac ttcaagtgga cgcgcgcggg ccgctccgcg ccgtgcgtct
      301 gccgcttctg gccctcgcgg cgatcctctt cgtcgcgtcc gccctggtgc tgtggcacga
      361 gacgcgcggg ctggccccgg gagacgggcc ctggcgatcg ccgggaccgt cgttcaccct
      421 cctatccccc gccggggcgg tcgcgacgcc gccctcggaa ctgcgctgga gcgccccgga
      481 cccgcaggcc gactgccgcg ccgagatcgt ccggctcgac ctcgccccgc tattcgagga
      541 gcgcgccgcg gccccgggac ggatccccct tcccgaaggg acgcgcgccg cgctggcctc
      601 gggagacgag ttcctctggc gcgtccgctg ttccggcgcg tccggcgagg agacctcctc
      661 ctatgtcggg ttccaggtcg tcacctcgcc ggcgccgggg agcgacggcg cgaccagaga
      721 atcgccgccg cggtgacgac cagaagcgcc gcacccagga tgagcaggcg cgccagcccc
      781 gcgccgatcc atggcaggcg cacgccggcg tccgggtccc cgacgtagac gaagggggcc
      841 cacgctcccg gatgacgcag gcgcgcgtcg cccgaagccg cgaattcgct acggaccgcc
      901 tgcagcgact ccgccgggct gccgcttcgg ccgagcgccg cgaggaagcg gctcatgaac
      961 cgggccgcca cgccgtcggg aacctcccac agcgtggcga ccgcgccgcg cgctcccgct
     1021 tcgaggaagg cagtcggaag cgcgatgacc ccttcggcgc ggtcggtcgg cccgacgccg
     1081 ctccggcacg ccgcgagcac gacgagcggg gcgttcaacc gcagcgacgg gatttcgcgc
     1141 atctgcagca ggtcgtcctc gaccggtcct cccggaccga ggaccaggcc ggagcggagc
     1201 gggtgggcgg tgtcggcgac gccgtgcgcg gcgatgtgca ggacatcgaa gccggtgaga
     1261 tcgatcgcga gcagcgccgt ctcgccggcc ccgatccccc ccgtgatgca gcgcgagccc
     1321 cagccgccgc gcgccgccca gcgctccgcc tcccgccttc cctcgggaag cggcgcgaac
     1381 cctggtcccg gatcgggggc gagggcgagg acccgtcccg tcggggatcc ggacgggcgc
     1441 gatcggagcg ccagccaggt cgccgccgac ggcaggtagg cggtgacgta gcgcccgccg
     1501 agtgtcgcgc cgccggcgcc gcggagggga agcgcctcga acgggacccg ctccagggcg
     1561 ccgtccggaa cgatcaggag gcggcgggtc ccctccggca gcatcccgat ccatggatcg
     1621 accagcgacc gcgccaggct ctcgagcgcc ggcgcgctcg ggggagcgtc ggtcgagtcc
     1681 ggcgggcggc gcagcagcgg ctccagtctc agggccatcg catcgatctc ctcccagtgc
     1741 ccgagcggca gcgatcggat cgccgtcccg tcgtgcagca gcgcgtaccc cccgccgggt
     1801 cccaggagaa acgtgacgag cgcggtgcgc gtctcgagga gcgccccgcc gagtgccgtg
     1861 agatcgagcg ccggcgcggg agaggccggc ggcggccggc ggagggcctc cgcctcgtcc
     1921 cgcgcggcgc gccgcaaccg atcctccgcg agcaggaggt ccgcttcgat gcgggcgcgg
     1981 gtcggggcat cgggggcgcc gcgcaggagc tccgactgca gcgccgagat ggcggcgtgc
     2041 gccgcccggg ccccctcacc gtcggagagg acgggggcga cgggacgcgg gcgcgacaga
     2101 gcctcgacga gccggcgcgc gcgactgtcg tcgaggaggc ggaaggcgtc gtccctccgc
     2161 ccggcggcgg agtcgggatc ggccgcggcc cggcgcagct gcagaagcgc cagccgcctc
     2221 caggcatcgg cgcgccgccg gtcgaggctc aggatcgact ccgtcgggag gatccgggcg
     2281 cggatctgcg agcgcagggc catcgcctcg cgccagtcgc gctccgcctc ctcgtcccgg
     2341 ccggccgctt cgaaaagctc gccgcgccat tcgagcgcgc cggcgacgta gtgcatccga
     2401 tcgaaggcgc gctccgcctc gacctgttcg tcggccgccc ggatcgcctc atcgcgatcg
     2461 ccgcgcgccc acgcgatgcg cgaccggttc gagtgggcct cgccggcggc gtccggatag
     2521 ccgcccggac gaccgggcag gcggacgagg gccaggcggt tccagcgtcc ggcggcgtcc
     2581 agatcgcccc gttcgatgtt cagggcggcg aggctgttga tcgcgtacag cgcctcgcgg
     2641 gtgcggtcgc cgcgatcgaa gtcgccgacc agccgctcga gccgctccgc cgcctcgtcg
     2701 aagcgccgcc ggtcggcgag gatgatcgcg aggtcgtatt cgcaggcgag cgcgtcctcg
     2761 cggttgccgg cggcctgcgc cacctccagc ccccgccggg ccagcgcctc cgcccgatcg
     2821 agctcaccga gaccgcggct caagagcgcc tcggtgatga acgggccgta gttctgcacc
     2881 ccttcccccg gcggcagtcg ctggatccga tcgagcgtgc gcatcgcctg ctccacctcg
     2941 ccgcgctgcg acagggtgct ggcgaggttc gacagcagga cggcggagag cccgggcggc
     3001 gcctgggcct tctccgcgag cgcgagggcg gtgcgcaccg cccgctcggc ggcggcgagg
     3061 tcgccggcgg tcgagtggat gtgcgccagc gatccgacca gctccgcctg tcccttgagg
     3121 tcgccgtggc gctgcgccag ctcgagccct tcgaccgtga tcgccagggc gcgatcgagt
     3181 tcgccgcgcc ggcgatgcag gatgccgagg gagttcagga ccgcggcgag gacctgatcg
     3241 tcctgcgccc ggc
//
LOCUS       DASRIQ010000064         4887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2893560, whole genome shotgun sequence.
ACCESSION   DASRIQ010000064 DASRIQ010000000
VERSION     DASRIQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893560"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VFD06_01655"
     CDS             complement(<1..1045)
                     /locus_tag="VFD06_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN02270.1"
                     /translation="MVLSLLAPPAVADGPAPGPQPTTLAQAPTPPPPPGTSPTEEPDT
                     SWLPKLEDPPYPGGAIIDSTPPNAIVLSLDDTIQQAMKNNLEIAVRSYDPQMFEQFAV
                     VERAIFDPVASGYVYASSQDSQQSFFLGALFVPASTTGDGDGYGASWFDPLTWGGNYR
                     VDVNIDNSDTDSLQADDPTTVIDESTSVNETLESNGWAFTYNQPLLRGLGRNANLWRY
                     IVASNTAQSSQAQFRRSVIDTLAVSEQSYWDLNFAIMQLRTSRFALKVAQDFLEQNKV
                     KVRVGTLAPIEITQAEAQVADRNETVILAEAGLRAAEDGVRLAIGMRKDSPDWARPVR
                     PSDPLAVNEVAPNE"
     gene            complement(1189..2163)
                     /locus_tag="VFD06_01660"
     CDS             complement(1189..2163)
                     /locus_tag="VFD06_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZN02271.1"
                     /translation="MSQPLLDVRDLVKRYPVRRGLFARVSAWVRAVDGVSLAIRPGET
                     LALVGESGSGKTTLGRCILRLLEPTSGSIRFDDSDFLALSPRELRRARRNLQVVFQDP
                     YGSLNPRMTVGAIVREPLDIHRIGRRGAEREGMVDALLQRVGLDPSMKRRYPHAFSGG
                     QRQRIGVARAIALRPKLIVADEPVSALDVSIQAQVINLLIDLQEEYRCAYLFIAHDLA
                     LVERFADRVAVMYLGRIVETAPARDLFTQPRHPYTKALLQAIPVPDPERVRDAAVVEG
                     VVPSAADLPPGCRFHPRCPIAVEACRQEDPPLREIAPGHFAACHLADG"
     gene            complement(2160..3200)
                     /locus_tag="VFD06_01665"
     CDS             complement(2160..3200)
                     /locus_tag="VFD06_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN02272.1"
                     /translation="MPPPLLRVEDLVVEFPGPLRAADGVSFTVGERETLGLVGESGCG
                     KSVTGLALLRLVPEPGCIVSGRILFRGDDLMVLSERQMCSVRGRGIAFVFQEPAAALN
                     PVLSVGTQIAEGLVVHRLMKKKEAMAEARRLLGLVRIPDAARRVHDYPHQLSGGMLQR
                     VMIAIALACRPALVIADEPTTALDVTIQAEILALLKRLRDELGLSLLLISHNLGVIAE
                     MADRVAVMYAGRIVEEAPVRSLFRDPAHPYTRALLRSMPRPGSSAAPGPRPRRLPAIE
                     GTVPDLARLPPGCAFHPRCPDVMPECRAAVPALLPLVAAAGAAAAHRVACYLHHDAAG
                     APRPRPLAEGVR"
     gene            complement(3203..4855)
                     /locus_tag="VFD06_01670"
                     /pseudo
     CDS             complement(3203..4855)
                     /locus_tag="VFD06_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130311.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
BASE COUNT          688 a         1732 c         1751 g          716 t
ORIGIN      
        1 cctcgtttgg agcgacctcg ttgacggcca gcgggtcgct ggggcgcacg ggccgggccc
       61 agtccgggga gtccttgcgc atgccgatcg ccaggcgcac cccgtcctcg gcggcgcgca
      121 gccccgcctc ggccaggatc accgtctcgt tgcgatccgc cacctgcgcc tctgcctggg
      181 tgatctcgat cggcgcgagc gtcccgacgc gcaccttgac cttgttctgc tcgaggaaat
      241 cctgcgccac cttgagggcg aagcgcgagg tgcgcagctg catgatggcg aagttgaggt
      301 cccagtagga ctgctccgag acggcgaggg tgtcgatgac gctccggcgg aactgcgcct
      361 ggctgctctg cgcggtgttc gacgcgacga tgtagcgcca caggttggcg ttcctgccga
      421 ggccgcgcag cagcggctgg ttgtaggtga aggcccagcc gttcgactcg agggtctcgt
      481 tgacgctggt cgactcgtcg atgacggtcg tcgggtcgtc cgcctggagg gaatcggtgt
      541 cggagttgtc gatgttcacg tccacccggt agttgccgcc ccaggtgagc gggtcgaacc
      601 acgacgcgcc gtatccgtcg ccgtcgccgg tggtgctggc gggaacgaag agcgccccga
      661 ggaagaacga ctgctggctg tcctggctgc tcgcgtagac gtagcccgag gcgaccggat
      721 cgaagatggc acgctcgacc accgcgaact gctcgaacat ctgcgggtcg taggagcgca
      781 ccgcgatctc gaggttgttc ttcatcgcct gctggatggt gtcgtcgagc gagagcacga
      841 tggcgttcgg cggcgtgctg tcgatgatcg ctccacccgg gtagggcgga tcctccagct
      901 tcgggagcca cgaggtgtcc ggctcctccg tcggcgacgt ccccggtggg ggcggcggcg
      961 tcggggcctg cgccagcgtc gtcggctgcg ggccgggcgc cggtccgtcg gcgaccgcgg
     1021 gcggcgccag gagggacagg accatcaaca ctcgtatcac ggccgtcgtt ctcatcaagt
     1081 cacctcgaag acaagcctgt cggtctcagt tgggtccgga cattctacgg acttatacgg
     1141 caggtggagc cgcgaaggtt ccgctgctcg caaaccccac gccagctcct atccgtcggc
     1201 caggtggcag gcggcgaaat gacccggcgc gatctcgcgc agggggggat cttcctgccg
     1261 gcaggcttcc acggcgatcg ggcagcgcgg gtggaagcgg cagcccggtg gcaggtcggc
     1321 cgccgagggg acgaccccct cgacgacggc cgcgtccctc acccgttccg gatccgggac
     1381 ggggatcgcc tgcaggagcg ccttcgtata aggatgccgg ggctgggtga agagatccct
     1441 cgccggggcc gtctcgacga tccgcccgag gtacatgacg gcgacccggt cggcgaaccg
     1501 ctccaccagc gccaggtcgt gcgcgatgaa gaggtaggcg cagcggtact cctcctgaag
     1561 gtcgatgagc aggttgatca cctgggcctg gatggagacg tcgagcgccg acaccggctc
     1621 gtcggcgacg atcagcttcg ggcgcagggc gatggcgcgg gcgactccga tgcgctgccg
     1681 ctgccccccc gagaaggcgt gcggatagcg ccgcttcatc gacgggtcga gcccgacgcg
     1741 ctgcagcagc gcgtcgacca tcccttcccg ctccgccccg cggcgtccga tgcggtggat
     1801 gtcgagcggc tcgcggacga tcgccccgac cgtcatgcgc gggttgagcg agccataggg
     1861 atcctggaag accacctgga ggttccggcg cgcccggcgc agctcgcgcg gcgacagcgc
     1921 caggaaatcg gagtcgtcga accggatgct gccggaggtc ggctcgagca ggcgcaggat
     1981 gcagcgcccg agggtcgtct tgccgctccc cgattcgccg acgagcgcga gcgtctcccc
     2041 cggccggatc gcgagcgaga cgccgtccac ggcgcgcacc cacgccgaca cccgcgcgaa
     2101 cagaccgcgg cgcacgggat agcgcttgac gagatcgcgc acgtcgagga gcggctgact
     2161 caccgcaccc cctccgccag aggccgcggt cggggtgccc cggcggcgtc gtggtgcagg
     2221 tagcaggcga cccggtgggc tgcggccgcg ccggccgccg cgacgagcgg gagcagcgcg
     2281 gggaccgcgg cgcggcactc gggcatcacg tccggacagc gcgggtggaa ggcgcacccg
     2341 ggcggcagcc gcgccaggtc cggcaccgtc ccttcgatgg ccgggaggcg gcgcggccgc
     2401 ggccccggcg ccgcagagga tcccggccgc ggcatggacc tcaagagggc gcgggtgtag
     2461 gggtgcgcgg gatcgcggaa cagcgaccgg accggcgcct cctcgacgat ccggccggcg
     2521 tacatgaccg cgacccgatc ggccatctcg gcgatgacgc cgaggttgtg gctgatgagc
     2581 aggagcgaca gcccgagctc gtcccgcagg cgcttcagga gcgcgaggat ctccgcctga
     2641 atggtgacgt cgagggcagt ggtcggctcg tcggcgatca cgagcgccgg cctgcaggcc
     2701 agggcgatgg cgatcatgac gcgctgcagc atccccccgg acagctggtg cgggtagtcg
     2761 tgcacgcgcc gcgcggcgtc ggggatgcgc accagcccca gcaggcgcct cgcctccgcc
     2821 atggcctcct tcttcttcat gagccggtgg acgacgagcc cctcggcgat ctgcgtcccg
     2881 acgctcagca ccgggttgag ggcggcggcc ggctcctgga agacgaaggc gatgccgcgg
     2941 ccgcgcacgg agcacatctg ccgctccgac aggaccatca ggtcgtcgcc gcggaagagg
     3001 atccgccccg agacgatgca ccccggctcg gggacgaggc gcaacagggc cagccccgtg
     3061 acgctcttgc cgcaccccga ttcgccgacg aggcccagcg tctccctttc cccgacggtg
     3121 aaggagacgc catcggccgc ccggagcggc ccggggaact cgacgacgag gtcctcgacg
     3181 cgcaggagcg gcggtggcaa cgtcaggcga gcctctcgcg cggcttgaag gcgccgaagg
     3241 cggcgcgcag ccggtcggag atctccccga gcgtggcccg cgcctcgacc gcggcgagca
     3301 gccgcggcat gaggttgtcg cggccgcggg ctgcctgctc caccgcgtcg agcgccgcgc
     3361 gggcggccgt cgcgtcccgc cctcccttca gggcccgcac ccgctcccgc tgcgcctcct
     3421 cgagcgcagg gtcgaggcgc aggaccggat acgcctgttc gcctcgcgcg tcgccgtcct
     3481 ggtagcggtt gacgccgacg acgacccgct cgccgcgctc ggtctgctgc tgggtgcggt
     3541 aggccgcctc ctggatctcc ttctgctggt agccggcctc gatcgcccgc accgttccgc
     3601 cctggcgatc gatcgcctcg aggtaccgga ccgcctccgc ctcgagccgg tcggtcgccg
     3661 cctcgagggc gtacgccccg ccgaacggat cgaccgtgtc ggcgacgccg gtctcgtgcg
     3721 ccaggatctg ctgcgtccgg agcgcgatgg tcgccgcctt ctcggtcggc agcgccagcg
     3781 cctcgtccat gccgttggtg tgcaggctct gcgcgccgcc gagcaccgcc gccagcgcct
     3841 gcacggcgac gcgggcgatg ttgttctccg gctgctatgc cgtcagcgtc gagccgcccg
     3901 tctgcgcgtg gaagcgcagc aggcgcgcct tcgggctggt cacgccgaag cgccgctcga
     3961 gcaggcgccc ccacagcctc cgggcggcgc ggaacttggc gatctcctcg aggaaattgt
     4021 tgtgcgcgtt gaagaaaaag gagatctgcc cggcgatctc ctccacagga agtccgcgct
     4081 ggcgcgccgc ctcgaggtag gtgaggccgt tggcgaaggt gaaggcgatc tcctgcgccg
     4141 ccgtcgcccc cgcttcacgg atgtggtagc cgctgacgct gatcacgttc cagcgcggca
     4201 tcgcctcgcg cccccacgcg aagatgtcgg tggcgaggcg cagcgagccc tccgggggat
     4261 agatgtacgt gccgcgcgcg atgtattcct tgagaacgtc gttctgcacc gtaccggaca
     4321 ggcgctcgtg cggcacgccg cgccgtgtcc cgaccgcggc atagagggcc agcaggatcg
     4381 ccgcggtggc gttgatggtc atcgaggtgg agaccttctc gagcggcagc ccctcgagga
     4441 gcgcctccat gtcctcgatc gagtcgatcg ccacgccgac gcgccccacc tcccccgtcg
     4501 ccatcgggtg atcggagtcg taccccatct gcgtcggaag atcgaaggcg atcgacagcc
     4561 cggtctggcc gcgatcgagc aggtagcgga ggcggcggtt ggtctcgcgc gcgtcgccga
     4621 acccggagta ctgccgcatc gtccagaagc ggccgcggta catcgtcggc tggatgccgc
     4681 gggtgaacgg cggctccccg ggcatcccga gcgcctcgtc gtactcccac ccctcgaggt
     4741 gctccggcgc gtacacgcgc tcgatcactc cccccgagtc gaaccggaac tcctcacgcc
     4801 gctccggaac cttttcgagc gaccccttga gagtcgcctc ctcccaggcc cgcagccgct
     4861 tcccaccttt cttccccggc gatttca
//
LOCUS       DASRIQ010000065         4563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1333145, whole genome shotgun sequence.
ACCESSION   DASRIQ010000065 DASRIQ010000000
VERSION     DASRIQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333145"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFD06_01675"
     CDS             <1..229
                     /locus_tag="VFD06_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HZN02273.1"
                     /translation="VLFAFLAGAAVGAATALIFAPASGRETRARVQRTAREVADKTRH
                     GVQEIKGSVGESWQRAVDAAKDAFEAALQRD"
     gene            422..940
                     /locus_tag="VFD06_01680"
     CDS             422..940
                     /locus_tag="VFD06_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02274.1"
                     /translation="MGSEDRDGAYLLSLSTYGHRPALAGSHAGLFCRVLARLHRRLDF
                     RLHAYVVLPDRARLIVATPDGRLGSIERLAQRLRSRFGRELQWRARWPGPVFRDGVVI
                     VPVGHGVSMARRVEFLHRLPVLMGLAGEPGDWRWSSVRGWTGSGRPPAPVDLPRPSPA
                     AKPDCAGSTPAA"
     gene            complement(957..1658)
                     /gene="lepB"
                     /locus_tag="VFD06_01685"
     CDS             complement(957..1658)
                     /gene="lepB"
                     /locus_tag="VFD06_01685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZN02275.1"
                     /translation="MTSVDTPKSLLRDYFETIVTCVVFVLFARTFVFQQSKIPTGSMI
                     PTLLIGDYIMVNKFVYAPRMAGVPLFGGLERALFPMRDVKRGDIVVFKYPEEPEKDYI
                     KRVIGLPGETIEVRDRQVYIDGQPLDEPYKVHRNQVRGLNIDQDDYPATRIPEGSYFC
                     MGDNRDNSRDSRSWGFVPASYLKGRAFMIWWSYEEDRDAWQQTAISDRLRGIWSKIAH
                     LCTKSRWDRTFQIIR"
     gene            complement(1741..2157)
                     /locus_tag="VFD06_01690"
     CDS             complement(1741..2157)
                     /locus_tag="VFD06_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02276.1"
                     /translation="MSPLARFVAPALILAAGLQISSAALAQNGTVREVPAPARRTARY
                     LSVKVWEAGAVAPNVSVRVPTALVSTTIALAAWTGALDHGFEQARIHAGRECGGPSIR
                     ITGRQIVSLWSDIVSGGPADLVRIDDGHDRVVIRLE"
     gene            complement(2236..2511)
                     /locus_tag="VFD06_01695"
     CDS             complement(2236..2511)
                     /locus_tag="VFD06_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HZN02277.1"
                     /translation="MTLVEAAAGLLNLAAAIQAAAPDAPFVAGEAAARPDQVVRGLRF
                     LTAAYTAVWVILAAYLLMLSVRQRRLERQLDRLRRRFGPDPTSPSGP"
     gene            complement(2508..3209)
                     /locus_tag="VFD06_01700"
     CDS             complement(2508..3209)
                     /locus_tag="VFD06_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HZN02278.1"
                     /translation="MLQRLPLTLAAIAVPLALQQAFLAAPPEQKMGVVQRIFYFHVPS
                     AMMAFLGVGLCAVLSALYLWKRDRRLDVAAEAAAELGVLFCTIVLITGPLWARPVWGV
                     YWTGEVRLTSTLILWLLFVGYLLLRGSAGGDRDATARFAAIVAIVGALDVPIIYKSVE
                     WWRGLHPKVLKVSGGGGLDPAMARSLLICTVAFLLLFAALYIERLRLGRLALEVASLE
                     ERSARLPDREGAVPA"
     gene            complement(3270..3950)
                     /locus_tag="VFD06_01705"
     CDS             complement(3270..3950)
                     /locus_tag="VFD06_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZN02279.1"
                     /translation="MTSTVRAALLIAGRDLRGELRRPGASLSLVVFALLVVVVFHFAF
                     DLTSAEFVALGPGVLWVAVLFTGVLALDRGFRVEREEGRIDGLLLAPMPRSALFFGKV
                     LSTCAVMAAVEILLVPLAAILFNHPFGGWLPRLLPVLMLNTVGFAAVGCLFACITTQT
                     RRGDLLLPILLFPASVPIALSAVRATGHILASRPLDRYAHWLALSAAYDLIFLAAAVL
                     LFDHALDD"
     gene            complement(3947..>4563)
                     /gene="ccmA"
                     /locus_tag="VFD06_01710"
     CDS             complement(3947..>4563)
                     /gene="ccmA"
                     /locus_tag="VFD06_01710"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HZN02280.1"
                     /translation="ERILLLGSNGAGKTTLLRVAATLLRPTAGSIAHFGAGTSEADRA
                     AARGGIGYLGHGSLLYDDLTVAENLRFHAGLHGLSDRAARVERALDEVGLRDRADDPA
                     GTLSRGLAQRLSAARAFLHRPRLMLLDEPFTGLDRPGVARLQAILRDRLGETGGCLLA
                     THDVVSAWPLVGRVVVLAQGRIAADRATAGLDPAGVASLPGLAP"
BASE COUNT          726 a         1583 c         1681 g          573 t
ORIGIN      
        1 cgtgctcttc gccttcctgg ccggagccgc cgtcggagcg gcgacggccc tgatattcgc
       61 gccggcctcc ggccgtgaga cgcgggcgcg ggtgcagaga acggcgcgcg aagtcgcgga
      121 caagacccgg cacggcgtcc aggagatcaa ggggagcgtg ggcgagtcct ggcagcgggc
      181 ggtcgacgcc gccaaggacg cgttcgaagc cgccctccag cgtgactgaa ccgaccgccg
      241 gcggacatgc cccgcggtcc ccggccggcc ccagcggccg gccgggggtc gcgcggactc
      301 cgcgccccgc cttccttccc acccggtttc ccagccctcc atcccgccgc gcatccctct
      361 tgcaaatccg aaccctcgcc gccgacgccg gcgccggaga gccgcgaggg gagggttcgt
      421 catgggatcg gaggaccggg acggagcgta cctgctgtcg ctctccacgt acggccacag
      481 gcctgcgctc gcgggctcgc acgcgggact tttctgccgt gtgctcgccc ggctgcaccg
      541 ccggctggat ttccgcctgc acgcttacgt cgtgcttccc gaccgggccc gcctgatcgt
      601 ggccacgccc gacggccgcc tcggctcgat cgaacggctg gcgcagaggc tgcgctcgcg
      661 tttcggccgc gagctgcagt ggcgcgcccg ctggcccggt ccggtgttcc gtgacggcgt
      721 ggtcatcgtg ccggtcggac acggcgtgtc gatggcgcgg agggtggagt tcctgcaccg
      781 gctgccggtc ctgatggggc tggcgggcga gcccggcgac tggcgctggt cgagcgtccg
      841 cggctggacc ggctccgggc gtccccccgc gccggtcgat ctgccgcgcc cgtcaccggc
      901 cgcgaagccg gactgcgccg gctcgacgcc ggccgcctga cgggacaggc tcctcgctaa
      961 cggatgatct ggaaggtgcg atcccagcgc gacttggtgc aaaggtgggc gatcttcgac
     1021 cagatgccac ggaggcggtc ggagatcgcc gtctgctgcc aggcgtcccg atcctcctcg
     1081 tacgaccacc agatcatgaa cgcccgcccc ttgaggtagg aggccgggac gaatccccag
     1141 gagcgactgt cgcgcgagtt gtcgcggttg tctcccatgc agaaatacga cccctcgggg
     1201 atgcgggtcg cggggtagtc gtcctggtcg atgttgagtc cgcgcacctg gttgcggtgc
     1261 accttgtacg gctcgtcgag cggctggccg tcgatgtaca cctgccggtc gcgcacctcg
     1321 atcgtctcgc cggggaggcc gatgacccgc ttgatgtagt ccttctccgg ctcctcgggg
     1381 tacttgaaga cgacgatgtc gccgcgcttc acgtcccgca tcgggaagag cgcccgctcc
     1441 agtccgccga agagcggcac cccggccatg cgcggcgcgt agacgaactt gttcaccatg
     1501 atgtagtcgc cgatgagcag tgtcgggatc atcgacccgg tcgggatctt cgactgctgg
     1561 aagacgaagg tgcgcgcgaa gaggacgaag acgacgcagg tgacgatcgt ctcgaagtag
     1621 tcgcgcagca ggctcttggg ggtgtcgacc gaggtcatcc cgtctccagg agggcgggat
     1681 tatagacgca tcgagggggg ggcgacgacc ggagggagac cggacctcgg aagccccccc
     1741 ctactcgagc cggatcacca cccggtcgtg gccgtcgtcg atgcggacga ggtcggccgg
     1801 gccgccgctc acgatgtcgg accagaggga gacgatttgc ctgccggtga tccggatcga
     1861 cgggccgccg cactctcttc cggcgtggat gcgggcctgt tcgaagccgt ggtcgagcgc
     1921 gccggtccag gccgcgagcg cgatggtcgt cgagacgagc gccgtcggga cgcggacact
     1981 cacgttcggg gcgaccgcgc ccgcctccca gaccttcacc gacaggtacc gggcggtgcg
     2041 ccgcgcgggg gccggaacct cgcggacggt gccgttctgg gcgagggctg ccgaagaaat
     2101 ctgaagcccg gcggccagga tcagggccgg agcgacgaag cgcgcgagcg gggacatggg
     2161 gcctcctctc ccaatcgctc gcgtgaaacg gcgatcgggg cggggcggtt cccgctcttt
     2221 acggcggcga ggctcctagg ggccggaggg ggaggtgggg tccgggccga atcgccgccg
     2281 gagccggtcg agctgccgct ccaggcggcg ctgccggacc gacagcatga gaaggtaagc
     2341 ggcgaggatg acccagacgg cggtgtaggc tgcggtcagg aaacgcagcc cccggaccac
     2401 ctggtcggga cgggccgccg cctcccccgc gacgaaggga gcgtcggggg ctgccgcctg
     2461 gatcgccgcc gcgaggttca ggagtccggc cgcagcctcg acgagggtca tgcgggaacc
     2521 gctccctccc ggtccggcag gcgcgccgac cgctcctcga gtgaggcaac ctcgagggcc
     2581 aggcgtccga gccggagccg ttcgatgtag agcgccgcga acaggagcag gaacgccacg
     2641 gtgcagatga gcagcgagcg cgccatcgcc ggatcgaggc cgccgccccc cgagactttc
     2701 agcactttgg gatggagccc gcgccaccac tcgaccgact tgtagatgat cggcacgtcg
     2761 agcgcgccga cgatggcgac gatcgccgcg aagcgggcgg tcgcgtcgcg gtccccgccg
     2821 gcgcttccac gcaggagcag gtagccgacg aacaggagcc acaagatcag agtcgaggtg
     2881 aggcgcacct cacccgtcca gtacaccccc cagacgggac gcgcccacaa cgggccggtg
     2941 atgagcacga tggtgcagaa gagcacgccg agctccgcag ccgcctctgc cgccacgtcc
     3001 agacgccggt cgcgcttcca gaggtagagc gcgctcagca cggcgcacag gccgactccg
     3061 aggaacgcca tcatcgcgga cgggacgtgg aagtagaaga tccgttgcac cacccccatc
     3121 ttctgctccg gcggggcggc gaggaacgcc tgctggagcg ccagcgggac ggcaatcgcc
     3181 gcgagcgtca gcggcaggcg ttgcagcatg gggcgggatg atacacccgg cggccgggac
     3241 cccgggaatg gcgccggtgc gttcgcgcct cagtcatcga gcgcgtgatc gaagagcagc
     3301 accgccgccg ccaggaagat cagatcgtag gcggcggaca gggccagcca gtgagcgtag
     3361 cgatcgagcg gccgcgacgc caggatgtgg ccggtggccc gcaccgccga cagcgcgatc
     3421 ggcaccgacg ccgggaagag caggatgggc agcagcagat cgccgcggcg cgtctgcgtc
     3481 gtgatgcagg cgaacaggca cccgaccgcg gcgaacccga cggtgttgag catcaggacc
     3541 ggcagcaggc gcggaagcca tccgccgaac ggatggttga acaggatcgc cgcgagcggc
     3601 acgaggagga tctcgaccgc cgccatcacc gcgcaggtgg acaggacctt gccgaagaac
     3661 agggcgcttc gcggcatcgg cgccaggagc agcccgtcga tgcgcccctc ctcccgctcg
     3721 acccggaagc cgcggtcgag cgccaggacc ccggtgaaca ggacggcgac ccacagcacg
     3781 cccggcccga gcgccacgaa ctcggcggag gtcagatcga aggcgaagtg gaagacgacg
     3841 acgacgagaa gcgcaaacac caccaggctg agcgaagcac cagggcggcg caactcgccg
     3901 cgcaggtcgc ggccggcgat gagcagggcg gcgcgcaccg tcgaggtcac ggcgccagcc
     3961 cggggagcga cgcgactccg gccggatcca gccccgcggt ggcgcgatcg gcggcgatcc
     4021 tcccttgcgc gaggacgacc acccgcccga ccaggggcca cgccgagacg acgtcgtgcg
     4081 tcgccaggag gcatcccccc gtttcgccga gacggtcccg gaggatggcc tggagccgcg
     4141 ccacgccggg ccgatcgaga ccggtgaacg gctcgtcgag cagcatcagt cgaggccggt
     4201 gcaggaaggc gcgggcggcc gaaaggcgct gcgccaggcc gcgcgacagc gtcccggccg
     4261 gatcgtcggc ccggtcccga aggcccacct cgtcgagggc gcgctccacg cgcgcggcgc
     4321 gatcggaaag gccgtgcagc cccgcatgga agcgcaggtt ctcggcgacg gtgagatcgt
     4381 cgtagaggag gctgccgtgt cccaggtagc cgatgccccc gcgcgccgcg gcccgatccg
     4441 cctcgctcgt tcccgcgccg aagtgggcga tcgacccggc ggtcggacgc aggagcgtcg
     4501 cggcgacgcg cagcagcgtg gtcttgccgg caccgttgct tccgaggagg aggatccgtt
     4561 cgc
//
LOCUS       DASRIQ010000066         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_24881, whole genome shotgun sequence.
ACCESSION   DASRIQ010000066 DASRIQ010000000
VERSION     DASRIQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24881"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFD06_01715"
     CDS             <1..930
                     /locus_tag="VFD06_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN02281.1"
                     /translation="VLTLEGVAKSYRTHLSLRKYWILKDLSFEVHPGEIFGFIGTNGA
                     GKTTTIKLVLGLLFPDSGTIRLCGEDPARPEARRRVGFLPENPYFHDYLTGAEFLDFH
                     ARLHGFRQPERRKRVGELLERVGLTNRADRALRLYSKGMLQRIGLAQALLHDPDVVIL
                     DEPMSGLDPIGRRDVRDLILELKRRGRTVFFSTHILSDTETICDRVGMLVRGRLAAIG
                     TVEELVRGEVESWEVTLAWPGPGNPPIGQVLSRRDDRVLLQVGSQADLHRLVASLNGG
                     GARLVAVSPARRSLEDLLVKEVRGATGAGERAS"
     gene            927..1694
                     /locus_tag="VFD06_01720"
     CDS             927..1694
                     /locus_tag="VFD06_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02282.1"
                     /translation="MSGRILAIALNTFREAIRNRILYLLLVFAVALILCAQALSLLTV
                     GSEEKIIKDFGLATIDLFGVLTAVLLGIGLVSREIERRTVYVLLSKPIHRAEFVLGKY
                     AGLVLTLLVNTAVMTACFLLLLFAKGIAAAGILAAIGLLFLQFLLITAIAVLFSCLSN
                     PIIACILTLALYVIGHLLWSFDLLRARLEAPWARALCAGLQRLLPDLAPFDVKGQVVH
                     GVPLPMDHVGYALVYLLLYGGAVLALACAAFQRKEMQ"
     gene            1691..2548
                     /locus_tag="VFD06_01725"
     CDS             1691..2548
                     /locus_tag="VFD06_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02283.1"
                     /translation="MRPGGVSGRGRSLVAAGLLLALSIAFAGRFGAMGRTSDAAPLLY
                     LPSGRYLQVVALGFDELLADALYLWSIQYYSNYAIADRYLYLEHIYKDVISELDPRFL
                     DPYLIGSMIMNVEAREPEMALRLLDKGIAKNPDQWILPFEAGFLCYQDLRDYPRASGY
                     FEQALAIPGVPPIVQRFKAEMARRGGDIRVSLQEWAAIHDAASDEYVRTIAWKHVHDL
                     RVQVDLEDLTAAVESYRSRFGRAPSGLAQLPAAGLLPALPRDPEGRPYLYDPRTGRPS
                     YGGLRILGR"
     gene            2545..>3128
                     /locus_tag="VFD06_01730"
     CDS             2545..>3128
                     /locus_tag="VFD06_01730"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZN02284.1"
                     /translation="MNGAAPDWLFPAFAGGLGAVVGSFLNVCIHRLPRDLSIVRPRSA
                     CPCCGRLIAWYDNVPILSFLLLRARCRGCSAPIAARYPLVEAATAGLFAALALLRGPG
                     IAFAAGAALAGALVALIVIDAEHQILPDAITLPGIAAGLFLAPLQAWLEPQGPGPGAV
                     LLDRLLGAVLGYLMLFLVNAAYRGWQGLQGVKPE"
BASE COUNT          392 a         1130 c         1064 g          542 t
ORIGIN      
        1 gtcctgacgc tggagggggt ggcgaagagc taccggaccc acctctccct gagaaagtac
       61 tggattctca aggacttgtc gttcgaggtg catccagggg agatcttcgg attcatcggc
      121 accaacggcg ccggcaagac cacgacgatc aagctcgtcc tggggctcct gttcccggat
      181 tccgggacga tccggctgtg cggcgaggat cccgcccgtc cggaggcgcg ccgccgcgtc
      241 ggcttcctgc cggagaaccc ctacttccat gactacctga ccggcgcgga gttcctcgac
      301 ttccacgccc ggctgcacgg gttccggcag ccggagcggc ggaagcgggt cggcgagctc
      361 ctcgagcggg tcgggctcac gaaccgggcc gaccgggcgc tgcggctcta ctcgaaagga
      421 atgctgcaga ggatcggcct ggcgcaggcg ctcctgcacg atccggacgt cgtcatcctg
      481 gacgaaccga tgtcgggact cgacccgatc ggccggcgcg acgtgcgcga cctcatcctc
      541 gagctgaagc ggcgcggccg caccgtgttc ttctccaccc acatcctctc cgataccgag
      601 acgatctgcg accgggtcgg gatgctggtg cgcggccggc tcgcggcgat cgggacggtg
      661 gaggagctgg tgcgcgggga ggtggagtcg tgggaggtca ccctcgcctg gcccgggccg
      721 gggaacccgc ccatcggcca ggtgctgtcg cggcgggacg accgggtgct ccttcaggtc
      781 ggatcgcagg cggacctgca ccgcctcgtc gcgtcgctca acggcggcgg ggcgcgcctg
      841 gtcgccgtct cgccggcgcg ccgctcgctg gaggacctgc tggtgaagga agtccgcggc
      901 gcgaccgggg cgggggagcg cgcgtcatga gcggccgcat cctggcgatc gcgctgaaca
      961 ccttccgcga ggcgatccgc aaccgcatcc tctacctgct gctcgtcttc gcggtggcgc
     1021 tcatcctgtg cgcccaggcg ctgtccctcc tgaccgtggg cagcgaggag aagatcatca
     1081 aggacttcgg cctcgccacg atcgacctgt tcggcgtcct cacggcggtg ctcctcggga
     1141 tcggtctcgt cagccgcgag atcgagcggc gcaccgtcta cgtgctcctg tccaagccga
     1201 tccaccgggc ggagttcgtc ctcggcaagt acgccgggct cgtcctgacg ctcctggtga
     1261 acaccgcggt catgaccgcg tgcttcctgc tgctcctctt cgcgaagggg atcgccgccg
     1321 ccggcatcct cgcggccatc gggctgctgt tcctccagtt cctgctcatc accgccatcg
     1381 cggtgctgtt ctcctgcctc agcaatccga tcatcgcgtg catcctgacg ctcgcgctgt
     1441 atgtcatcgg ccatttgctg tggagcttcg atctcctgcg cgcccggctc gaagcgccct
     1501 gggcgcgcgc gctgtgcgcc gggctgcagc gcctcctgcc ggatctcgcg ccgttcgacg
     1561 tcaaggggca ggtggtgcac ggagtcccgc tcccgatgga ccacgtcggc tacgccctgg
     1621 tttacctgct cctgtacggt ggggccgtgc tggcgctcgc gtgtgccgcg ttccagcgca
     1681 aggagatgca gtgaggccgg gcggcgtgtc ggggcgcggg cgctccctcg tcgccgccgg
     1741 gctcctgctg gcgctgtcga tcgccttcgc gggacggttc ggtgcgatgg ggcggacgag
     1801 cgacgcggcg ccgctcctgt acctcccgtc cgggcgatac ttgcaagtgg tggcgctggg
     1861 gttcgacgag ctgctcgccg acgctctgta cctctggtcg atccagtact acagcaacta
     1921 cgctatcgcc gaccgctacc tctacctgga gcatatctac aaggacgtca tcagcgagct
     1981 cgacccgcgc ttcctcgacc cctatctcat cggctcgatg atcatgaacg tcgaggcgcg
     2041 cgagccggag atggcgctgc gcctcctcga caaggggatc gcgaagaacc cggatcaatg
     2101 gatcctgccg ttcgaggccg gcttcctctg ctaccaggac ctgcgcgact atccgcgcgc
     2161 ctccggctac ttcgagcagg ccctcgccat cccgggtgtc ccgccgatcg tgcagcgctt
     2221 caaggcggag atggcgcgac gaggcggcga catccgcgtc tccctgcagg agtgggccgc
     2281 catccacgac gccgcgtccg acgaatacgt caggaccatc gcctggaagc acgtccacga
     2341 cctgcgggtg caggtcgatc tcgaggacct caccgcggcg gtcgagtcgt accggtcgcg
     2401 cttcgggcgc gccccgtccg gtctggcgca gctcccggcc gcgggactcc tgccggcgct
     2461 cccgcgcgat cccgaggggc ggccctatct ctacgatccg cggaccggcc ggccgtcgta
     2521 cggtgggttg aggatcctgg gacggtgaac ggcgccgctc cggactggct ctttccggcg
     2581 ttcgccggcg gcctcggcgc cgtcgtcgga tcgttcctca acgtctgcat ccaccggctg
     2641 ccgcgtgatc tctcgatcgt ccggccgcgc tccgcctgcc cgtgctgcgg gcggctcatc
     2701 gcctggtacg acaacgtgcc gatcctctcc ttcctcctcc tgcgcgcccg ctgccgcggc
     2761 tgcagcgcgc cgatcgcggc gcgctacccg ctggtcgagg cggcgacggc cggtctgttc
     2821 gcggcgctgg cgctcctgcg cggtccgggg atcgccttcg cggcgggggc cgcgctcgcg
     2881 ggggcgctcg tcgccctcat cgtcatcgac gccgagcacc agatccttcc ggatgccatc
     2941 acccttccgg gaatcgccgc cggcctcttc ctggcgccgc tccaggcctg gctcgagccg
     3001 cagggtcccg gccccggcgc ggtcctgctc gatcggctgc tcggggccgt cctcggctac
     3061 ctgatgctgt tcctcgtcaa cgccgcctac cgcggctggc aggggctcca gggggtgaag
     3121 ccggagga
//
LOCUS       DASRIQ010000067         4523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_25102, whole genome shotgun sequence.
ACCESSION   DASRIQ010000067 DASRIQ010000000
VERSION     DASRIQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25102"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFD06_01735"
     CDS             complement(<1..477)
                     /locus_tag="VFD06_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HZN02285.1"
                     /translation="MSASETLTSLLNEGAVLAVRLGPGEDLPAACLSAVRGGIGALEI
                     TLTTPGALGAIDALAKESGVLAGGGTVLTAEDVRKVARAGGRFALSPIFDREAADEAR
                     AADILYIPGAATPTEILAAHRYGAPVVKFFPSGALGGPAFMRAFRGPFPGIPLLPTS"
     gene            complement(515..1927)
                     /locus_tag="VFD06_01740"
     CDS             complement(515..1927)
                     /locus_tag="VFD06_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HZN02286.1"
                     /translation="MADIPPTLRVLFARSTLAVTGALLTTASAVLFLVLFAMERIGWF
                     HSNPYSGLIVLVLLPSLFVLGLLLIPLGIYLAIRRSRRGTLAEPSWPVLDLNRARVRH
                     GILLLLVLTVVNVVILSIGSYGALEHMDSPSFCGQTCHVPMQPQYEAWQHGPHSNVAC
                     VECHIGSGASAFVQYKWNGVHQMVGVVTGRFPRPIPAPVHNLRPASEICSPCHSSWKG
                     HGERAVVVHEYADDVAGTETVTNLAMHVTRGEAAALPDGGRHWPRGSGIHWHADSGNR
                     VEYASDEKRTQILWMKVTTPDGTTREYTAEGADPAAVAAAGRRVMDCVDCHNQPVHQF
                     PVSAARVVDQAIAAGRLRRDLPFVRREAVRLLGTEAPTQEDGLKAIEAGLRAFYAPGG
                     GAPAGAGADPAVLDQTVRAVQEIYRRSVFPSMKVTFGTYPNHLGHADNFGCMRCHDQS
                     HAMADGTVLPSDCDLCHTMP"
     gene            complement(1938..4397)
                     /locus_tag="VFD06_01745"
     CDS             complement(1938..4397)
                     /locus_tag="VFD06_01745"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HZN02287.1"
                     /translation="MAEPEEAPPQDPATEPQAPVTQMPDAPSASPASVHPSTLPSTLP
                     AAGMITGEVTVGGQGLSEDPPNSAKFFEYRDVPSGFVAESVNIFWTPRVRSFFAVNAY
                     DITQDDSRGAVEFGTFDQWRGSIHWVNNPRLWGDDPEQLWARQGDAVFTLDNTLQSAV
                     QAAPAPVDTTPADGQWDAGTKGALIKSAIDQSAQDVELGHLRKVAGASLVWTPVRQWA
                     FGVDATRERRTGTAPQSLGNYFSLAPGEVAAPVDFKTDWANLRAEYRGNTWNAGGSIG
                     VSKFETGYHDLTWDNQLSLVDQAVSPTLTSIPGTERLLLGTDNEALTATLYGGWNLPG
                     RTRVDATVSATDVTQDDPFLTMTVNTQLQPLLAPLPAESLDGEHRYSLLQLRASGRPT
                     NEFRWNVWARQYELENESPSLIFPEYVQTDYAIPLCGSIAACGATTNRIARRNLPYAY
                     GKDSYGALVGWRPTGWFDGSFSYELIDMSREFAAVEESQEDRYKLTLDFQAGEQFTIR
                     TTAVRAERRADHYEAEYNLASFPIGEPSLAPSNEGMRKFFWTDRDRDVYSVQVEWTPS
                     ENWSVYAEASWAENTYLDPNTGQEVGTSFDVQEDRLPGSPGVPDGVPETYTLLLAGRT
                     FDKDQSYSLGASFSPSNRFAIFADYTWEQWEYRLESRYRNVSAGVGTDNPLDDWGSDA
                     DDKYRTATLGLHAELNADLRWQLDVDATWARGTGDLTTDFVPGGSASGDTTLTEFPQL
                     QNTFKLARAQLTHVVSPRVSWLVRYWYESWNEDNWASDFMQPYMGDPGNDAGSSTAAY
                     LGFDFDDYTYQVVSAFLRFGF"
BASE COUNT          646 a         1528 c         1658 g          691 t
ORIGIN      
        1 ggaggtcggc aggagcggga tgccggggaa cgggccgcgg aaggcgcgca tgaaggccgg
       61 acctcccagc gccccgctcg ggaagaactt caccaccggc gcgccgtagc ggtgcgccgc
      121 caggatctcg gtcggggtcg ccgcgccggg aatgtagagg atgtctgcgg cgcgcgcctc
      181 gtccgcggcc tcgcggtcga agatggggga gagggcgaac cggccgccgg cgcgcgcgac
      241 cttgcgaacg tcctcggccg tcaggaccgt cccgcccccc gcgagcaccc ccgactcctt
      301 cgccagcgca tcgatggcgc cgagcgctcc gggcgtcgtg agggtgattt cgagcgcgcc
      361 gatgccgccg cggaccgccg acaggcaggc cgcggggagg tcctccccgg gccccagccg
      421 caccgccaga accgcccctt cgttcaggag cgacgtgagt gtctcggaag cgctcatcgg
      481 ttcgacgccg cgggacaggc tcccggccgg cggctcaggg cattgtatgg cacaggtcgc
      541 agtcggacgg caggaccgtc ccgtcggcca tcgcgtgcga ctggtcgtgg cagcgcatgc
      601 agccgaagtt gtcggcgtgg ccgaggtggt tcgggtaggt cccgaaggtg actttcatcg
      661 aagggaagac acttcgccta taaatctcct ggaccgcgcg gaccgtctgg tccagcaccg
      721 ccgggtctgc ccctgccccg gccggtgcgc cgccgccggg ggcgtagaac gcgcgcaggc
      781 cggcctcgat cgccttcagc ccgtcttcct gcgtcggcgc ctctgtcccc aggaggcgga
      841 ccgcttcgcg ccgcacgaac ggcagatcgc ggcgcagccg gccggcggcg atcgcctggt
      901 ccaccacccg cgccgccgac accgggaact ggtgcaccgg ctggttgtgg cagtcgacgc
      961 agtccatcac ccggcgcccg gcggccgcga cagccgccgg atccgcccct tcggcggtgt
     1021 actcgcgcgt cgtcccgtcg ggcgtggtca ccttcatcca gaggatctgg gttcgtttct
     1081 cgtcggaggc gtactccacc cggttgccgg agtcggcgtg ccagtgaatg ccggatcccc
     1141 gcggccagtg ccgcccgccg tccgggagcg cggccgcctc ccccctggtg acgtgcatcg
     1201 ccaggttggt cacggtctcg gtccccgcca cgtcgtcggc gtactcgtgc acgacgacgg
     1261 cccgctcgcc gtgccccttc cacgacgagt ggcacgggct gcagatctcc gaggcgggtc
     1321 tgaggttgtg gacgggggcc gggattggcc gtgggaatcg gcccgtgacg accccgacca
     1381 tctggtgcac gccgttccac ttgtactgca cgaacgccga cgccccggag ccgatgtggc
     1441 attcgacgca ggcgacgttc gaatgcggcc cgtgctgcca ggcctcgtac tgcggctgca
     1501 tcggcacgtg gcaggtctgg ccgcagaagg agggggagtc catgtgctcg agagccccgt
     1561 acgagccgat cgacaggatg acgacgttga cgaccgtcag gaccaggagg agcaggatgc
     1621 cgtggcgcac ccgcgcccgg ttcaggtcga ggaccggcca cgagggctcg gcgagcgtcc
     1681 cgcgccggct ccgccggatc gcgaggtaaa tcccgagcgg aatgagcagg agccccagga
     1741 cgaacaggga cggcaggagg acgaggacga tgagcccgga gtagggattg ctgtggaacc
     1801 acccgatcct ctccatcgcg aagaggacga ggaagaggac ggcgctggcg gtggtgagca
     1861 gcgccccggt gaccgcgagc gtgctgcgcg cgaacaggac gcgcagcgtc ggtggaatgt
     1921 ccgccactag gctcccacta gaacccgaag cgcaggaagg cgctcaccac ctggtaggtg
     1981 tagtcgtcga agtcgaagcc gagataggcg gccgtggacg agcctgcgtc gttgccgggg
     2041 tcccccatgt acggctgcat gaagtcggag gcccagttgt cctcgttcca cgactcgtac
     2101 cagtagcgga cgagccacga cacccgcggg ctgacgacat gcgtcagctg cgcccgggcg
     2161 agcttgaagg tgttctgcag ctgcggaaac tcggtcagag tggtgtcgcc gctggcggat
     2221 ccgcccggga cgaaatcggt ggtcaggtcc ccggtgccgc gcgcccaggt ggcgtccacg
     2281 tcgagctgcc agcgcaggtc ggcgttcagc tccgcgtgca gcccgagcgt ggcggtgcgg
     2341 tacttgtcgt cggcgtcgct cccccagtcg tcgagcgggt tgtcggttcc gacgccggcg
     2401 gagacgttgc ggtaccggct ctccagccgg tactcccact gctcccacgt gtagtcggcg
     2461 aagatcgcga accggttcga cggggagaag gacgcgccca ggctgtacga ctggtccttg
     2521 tcgaacgtgc gcccggcgag gagcagcgtg taggtctccg gcacgccgtc cggcactccc
     2581 ggcgacccgg gaaggcgatc ctcctggacg tcgaacgacg taccgacctc ctggcccgtg
     2641 ttgggatcga ggtacgtgtt ctccgcccac gacgcctcgg cgtagaccga ccagttctcg
     2701 ctcggggtcc attcgacctg caccgaatag acgtcgcgat cccggtcggt ccagaagaac
     2761 ttgcgcatcc cctcgttgct cggggcgagg gacggctcgc cgatcgggaa cgacgccagg
     2821 ttgtactccg cctcgtagtg gtcggcgcgc cgctccgccc tcaccgcggt cgtgcggatg
     2881 gtgaactgct cgcccgcctg gaagtcgagg gtcaacttgt agcggtcctc ctgcgactcc
     2941 tcgacggcgg cgaactcgcg cgacatgtcg atgagctcgt aggagaagga cccgtcgaac
     3001 cagccggtcg gcctccagcc gacgagtgcg ccgtaggagt ccttcccgta ggcgtaaggg
     3061 aggttgcggc gcgcgatccg gttggtggtg gcgccgcagg ccgcgatgct gccgcacagc
     3121 gggatggcgt agtcggtctg gacgtattcg gggaagatca ggctgggcga ttcgttctcc
     3181 agctcgtact ggcgcgccca gacgttccag cggaactcgt tcgtgggccg ccccgaggcc
     3241 cgcagctgga gcaggctgta gcgatgctcg ccgtccagcg actcggcggg aagaggggcc
     3301 agcagcggct gcagctgcgt gttgacggtc atcgtcagga acgggtcgtc ctgggtgacg
     3361 tcggtcgccg acacggtcgc gtcgacgcgg gtccggcccg ggagattcca gccgccgtag
     3421 agcgtcgcgg tgagcgcctc gttgtcggtg ccgagcagga gccgctcggt ccccgggatc
     3481 gaggtgagcg tcggcgacac cgcctgatcg accagcgaca gctgattgtc ccaggtcagg
     3541 tcgtgatacc cggtctcgaa cttcgagacg ccgatcgatc cgccggcgtt ccaggtgttg
     3601 ccgcggtatt cggcgcgcag gttggcccag tcggtcttga aatcgaccgg ggccgcgacc
     3661 tcgcccgggg cgaggctgaa gtagttgccg agcgactgcg gggcggtgcc ggtgcggcgc
     3721 tcgcgcgtcg cgtccactcc gaacgcccac tgccgcacgg gggtccagac gagcgacgcg
     3781 ccggccacct tgcgcaggtg cccgagttcg acgtcctgcg ccgactggtc gatggcgctc
     3841 ttgatgagcg cccccttggt gccggcgtcc cactggccgt cggcgggcgt cgtgtcgacc
     3901 ggggcggggg ccgcctgcac cgccgactgc agcgtgttgt cgagggtgaa gaccgcgtcc
     3961 ccctgccgcg cccacaactg ctcggggtcg tccccccaga ggcgcggatt gttgacccag
     4021 tggatcgagc cgcgccactg gtcgaaggtg ccgaactcga ccgccccccg gctgtcgtcc
     4081 tgcgtgatgt cgtaggcgtt gaccgcgaag aaggaccgga cccgcggggt ccagaaaatg
     4141 ttgacgctct ccgccacgaa gccgctcggg acgtcgcggt attcgaagaa cttcgcggag
     4201 ttcggcgggt cctcgctgag cccctgacct ccgaccgtga cctcgccggt gatcatgccc
     4261 gccgcgggaa gcgtcgaggg gagcgtggac ggatgcaccg aggcggggga cgcggaaggc
     4321 gcatccggca tctgcgtcac gggtgcctgc ggctcggtgg ccgggtcctg cggcggcgcc
     4381 tcctcgggct ccgccatccc cggcccttcc tccggctcgg gcggggtctc gggctgttcg
     4441 ggcggaggtt cgggctgttg gggcggcggc ggaggggccg ggtcctgcgc gatgagtggc
     4501 ggcgcgacca tcagcgtcag gag
//
LOCUS       DASRIQ010000068         9315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_801330, whole genome shotgun sequence.
ACCESSION   DASRIQ010000068 DASRIQ010000000
VERSION     DASRIQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801330"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /gene="mraW"
                     /locus_tag="VFD06_01750"
     CDS             <1..586
                     /gene="mraW"
                     /locus_tag="VFD06_01750"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HZN02288.1"
                     /translation="DHGGTTAADLVNGLPEPALRDLLARYGEEPHAARIARAVARERA
                     RAPFLRTLRLAEVVAAAVPAPARRAAAKRTAARPGGARVHPATRTFQALRIAINGEIE
                     GLPALLEGSIERLRPGGRLAVIAFHSLEDRAVKETFRSLAHRCVCPRGLPLCGCGRPD
                     IVRSVGSGAAQVAEDERSANPRSRSARLRAVERL"
     gene            583..1077
                     /locus_tag="VFD06_01755"
     CDS             583..1077
                     /locus_tag="VFD06_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HZN02289.1"
                     /translation="MNRARAAAAQIQVNQRLVRERDRQRSRELWRFLLTAAVLAVPVL
                     GYVWQRVDFLRVSYHYERLQKERQTLLEQSEQLKVERATLMDHDRIERLARQRLGLVD
                     PEPDDVRNVRLFDGRVGAVERTIEAGAIAAPIPVISKRPATAPPAGRNASGSAAGTRA
                     KGRP"
     gene            1106..3343
                     /locus_tag="VFD06_01760"
     CDS             1106..3343
                     /locus_tag="VFD06_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZN02290.1"
                     /translation="MSRLRRRFQRRAADGTATPAGLLRGARFRRGPVPGPPSERARRE
                     RLAILIAMISIGFLGMAARCAHLQIVRAPDLLALARSQHEKAVDLDPRRGPILDRNGQ
                     ELALSIDVDSLYADPSKVADAAAAARRLAPVLGMKASEIRERLDGGKHFAWVRRKVDP
                     ALRARVESLGIEGIAFVRESRRFYPKRDLAAHVVGACGVDNQGLAGLEFAFNERFTGT
                     PGRLEFLRDGRGGRVLDRSRVEPVPGDGMILTLDAAIQHIAERELDVAMRETRADGAA
                     IVVLRPETGEILALASRPGFDANEFPEASVTARRNRAVSDFFEPGSTFKVITAAAALD
                     AGRVRPGEVIWCENGSYVVAKHRFKEDRLPFGNLTFTEVLARSSNVGTIKVAQRLPST
                     DFIEVIRRFGFGRSTSLELPGESPGLLRDLKRWSGLSHASISMGQEIGVTPIQLAAAL
                     GALANDGMYNPPHLIEAFVAADGSRTEPERDGDFEARRAVSAKTARTLRDMLQSVTID
                     GTGQAAQVVGYTTGGKTGTAQKIDPATRRYARGKYAAWFGGFVPAERPALVLVVMVDE
                     PKGIHQHGGDVSAPVFSRVAEPVLRYLGVLPDREDPLVIDAPLVAAARPAAPAVQPAS
                     LRTSSRKEGSAVPASRTAPAGAAPSDARSFGRKILDAATRTIPVAGGTVQAGFIGSPA
                     GADEGREVVMPDLTGMSLKQATEALGRVGLNCSSRLTGPRVSRQEPVAGLAIRPGARC
                     SIILE"
     gene            3352..4896
                     /locus_tag="VFD06_01765"
     CDS             3352..4896
                     /locus_tag="VFD06_01765"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZN02291.1"
                     /translation="MQLQAVLRDIAGNQVSGDQTTAIRGIAYDSRQVEPGFLFAALEG
                     EARDGHDFLDQARARGAVAVLSARPRDPRFPGLAWVQVEDARRGLALASRNYYGRPDE
                     RLMMLGVTGTNGKTTVCHLLEGGLREAGLRPCLLGTVSYRYGKDETRADRTTPESLDL
                     QRHLDRFATAGARSCAMEVSSHALALRRVAGIAYRAAVFTNLTQDHLDFHSTMEEYFQ
                     AKALLFRSLKPDAVAVLNADDPAAERLAAVTGARVVTFGESARVHLRLSEVRATLAGT
                     EVTLTPSRGLTDRGTEDGPIAVRSPLLGRHNASNLAAAAATLLSVGVPAEAVPKGLAA
                     VTGVPGRMERVDDGSAFIVLVDYAHTDDALTHLLESVRALQPKRVITVFGCGGDRDRD
                     KRPKMGFAAASHSDIVVVTSDNPRTEDPHAIVRAILPGVARALGVDPGAPLPADRCHV
                     LVDRREAIHRAVSLARPGDAVVIAGKGHEPYQILGERTVPFDDRQVARDALHGGGAVR
                     GRAACE"
     gene            4898..6337
                     /gene="murF"
                     /locus_tag="VFD06_01770"
     CDS             4898..6337
                     /gene="murF"
                     /locus_tag="VFD06_01770"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZN02292.1"
                     /translation="MARLTLGEAAKAMSGRMSITERERGRVGETTTVTGYSIDSRTLK
                     AGDLFFAIVGPRVDGHDFARQAAEKGAAAVVVSKGGPGRYPEAPAVLRVDDTTKALQD
                     LGGHVRRRRPLKVVGITGSAGKTTAKEMTGSVLDRRFKTHRSEGNLNNAWGLPLTLLR
                     MQDDVEAAVLEMGMSYKGEIARLVEISNPDVGVILNVLPVHLEHFGSIAGIAAAKGEL
                     FRGLRRDAIAVYNADDARVARLGKAFKGTTLPYGIAARRAEVKADEVVSLGLKGTRFT
                     LRYGDMRAPVRLAIPGRHHVYNALAAAATGFACGLDPSDIAAGLVRMRPAAMRGVLHR
                     RQDGVEILDDSYNSNPAAMERAIELLAETAPRGRRILAAGDMLELGPASARAHARIGT
                     LAARAGIDLLVAVGPLSKRMAQAAAPKAPRKAAATKVRHCDSSEEAATYLASEIRPGD
                     LVLVKGSRGIRMERIVRALLGEGTTGGTH"
     gene            6337..7422
                     /gene="mraY"
                     /locus_tag="VFD06_01775"
     CDS             6337..7422
                     /gene="mraY"
                     /locus_tag="VFD06_01775"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZN02293.1"
                     /translation="MLYSLLYPLHETFSMFNVFRYITFRTALATLTALLISLALGPRL
                     IQRLRHFQIGQQIRPEGPLSHQSKKGTPTMGGLLILIAVILPTLLWADPANPYVWIAL
                     ISTLLYGVIGFADDYLKLTRKRSLGLTARQKFAAQMVVALGIGLVLLWMTKQGFYSTH
                     LSLPFFKRFDPDLGWVFVLWTMLVVVGAANAVNLTDGLDGLAIGSSLIASGTYAILAY
                     VAGHAKVSAYLDVPNVRGAGELAVICGALVGASLGFLWFNCNPAQVFMGDVGSMAIGG
                     GLGTIAILIKQEVLLIFVGGLFVIEALSVIAQVGSFKLRGKRVLRMAPIHHHFELAGW
                     PEQKVVIRFWIVALIFALLGLSTLKLR"
     gene            7430..8818
                     /gene="murD"
                     /locus_tag="VFD06_01780"
     CDS             7430..8818
                     /gene="murD"
                     /locus_tag="VFD06_01780"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZN02294.1"
                     /translation="MMPAPRPPRTAPDLRGARVVVAGAAKSGVALARFLLARGASVTL
                     SDQRQARDLGPEVAALAARGVAFEFGRHEEATFSGADLVVVSPGVPLSIQPLAAARRR
                     GVRVVAEVEVASWYLEGTLIGITGSNGKSTTTALAGHLLARAGRNAVACGNLGTPLIE
                     TLEQDAPDRLYVVELSSFQLEGIETFRPHVSVLLNLSPDHQDRYPDTAAYYAAKGRLF
                     MNQKAADHAVLNQDDEAVALLARGIAPRRHGFSRAGRVADGAYVRGADILLVEDGREQ
                     PVLPVKDLPLFGVHNQENVMAALLVARLCGVAAEQAADGVRSFRGLPHRLERVRDLGG
                     VSWFNDSKATNVGAAAQSLRSFAGGVVLIMGGKDKGGTFADLTSLVRDRVTHLVLLGK
                     AKETIARQIGPIVPTILVEDMEDAVAAARVAALPGGVVLLAPGCASFDQYSGFEARGE
                     HFRSLVNALEET"
     gene            8821..>9315
                     /locus_tag="VFD06_01785"
     CDS             8821..>9315
                     /locus_tag="VFD06_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZN02295.1"
                     /translation="MARKLTFDRPLFVVGIGLALFGLVMIFSASAPLAMERHQTPYHF
                     LMRQMLAFAIGLCGMAVAMNFDYRALLKRPAVGLAIGGAALLLAGVLVVDRTHQVHRW
                     FKVGPIQVQPSEIAKIALVMFLAAQMAKHENRVNDIARSLLPTAVVVVPLAGLVYLEP
                     DLGTA"
BASE COUNT         1250 a         3410 c         3326 g         1329 t
ORIGIN      
        1 cgatcacggc gggaccaccg ccgccgacct ggtgaacgga ctgcccgagc ccgcgctccg
       61 cgatctcctc gcgcgctatg gggaggagcc gcacgcggcc cgcatcgccc gcgccgtggc
      121 gcgcgagcgc gcgcgggcgc cgtttctccg gacgctgcgt ctggcggagg tcgtcgccgc
      181 cgccgtcccc gcccccgccc gccgcgccgc ggcgaagcgg acggcggcgc ggcccggcgg
      241 cgcgcgcgtc catccggcca cccgcacctt ccaggcgctg cgcatcgcca tcaacggaga
      301 gatcgagggg ctgccggcgc ttctcgaggg gtcgatcgag cgcctccgcc ccggggggcg
      361 cctggcggtg atcgccttcc actcgctcga ggaccgcgcg gtcaaggaga ccttccggtc
      421 gctcgcgcat cgctgcgtct gcccgcgtgg tctgccgctc tgcggctgcg gccggcccga
      481 catcgtgcgc tcggtcgggt ccggggccgc gcaggtggcc gaggacgagc ggagcgccaa
      541 tccgcgcagc cgatccgccc gcctccgggc ggtggagcgc ctgtgaaccg cgcgcgcgcc
      601 gcggcggcgc agatccaggt gaaccagcgc ctggtgcgcg agcgcgaccg ccagcgctcg
      661 cgcgaactgt ggcgcttcct gctcacggcg gcggtgctgg cggtgccggt gctcggctac
      721 gtctggcagc gggtcgattt cctgcgcgtc tcctaccact acgagcgcct gcagaaggag
      781 cgccagacgc tcctcgagca gagcgagcag ctcaaggtcg agcgcgccac cctcatggac
      841 cacgaccgca tcgagcgcct ggcgcggcag cgcctcgggc tcgtcgatcc ggagccggac
      901 gacgtgcgca acgtccggct gttcgacggg cgggtcgggg cggtcgagcg gacgatcgag
      961 gccggggcca tcgccgcgcc gatcccggtc atctcgaagc gccccgccac ggcaccgccg
     1021 gccggccgca acgcctcagg gagcgccgcc ggcacccgcg cgaaagggag accttgagac
     1081 gctcgagacg ccccacggtg cgggcatgag ccggctgcgc aggcgcttcc agcgccgtgc
     1141 cgccgatggc acggccaccc cggccgggct cctgcgcggg gcgcgcttcc gccgcggccc
     1201 ggtccccggc cccccgtcgg agcgggcgcg ccgggagcgg ctggcgatcc tcatcgccat
     1261 gatctcaata ggtttcctcg ggatggcggc ccgttgcgct catctgcaga tcgtccgggc
     1321 gccggacctc ctggcgctcg cccgctcgca gcacgagaag gcggtcgacc tggacccgcg
     1381 ccgcggcccg atcctcgatc gcaacggtca ggagctggcc ctctccatcg acgtcgactc
     1441 cctctacgcc gacccatcga aggtcgccga cgcggccgca gcggcgcgcc gcctggcgcc
     1501 ggtcctcggc atgaaggcat ccgagatccg cgagcgcctc gacggcggga agcacttcgc
     1561 ctgggtgcgt cgcaaggtcg atccggcgct gcgcgcccgc gtcgaatcgc tcgggatcga
     1621 ggggatcgcc ttcgtgcgcg agagccgccg cttctacccg aagcgggatc tcgcggcgca
     1681 cgtcgtcggc gcctgcgggg tggacaacca ggggctcgcc gggctcgagt tcgccttcaa
     1741 cgagcgcttc accggcacgc cggggcgtct ggagttcctg cgggacgggc gcggcggccg
     1801 ggtgctcgac cgctcgcgcg tcgagccggt gccgggggac gggatgatcc tgacgctcga
     1861 cgccgccatc cagcacatcg ccgagcggga gctcgacgtg gcgatgcggg agacgcgcgc
     1921 cgacggggcc gccatcgtcg tgctgcgccc ggagaccggg gagatcctgg cgctcgcctc
     1981 ccgccccggc ttcgacgcca acgagttccc ggaggcgtcc gtcacggcgc gccgcaaccg
     2041 cgccgtctcg gacttcttcg agcccgggtc gaccttcaag gtgatcaccg ccgcggcggc
     2101 gctcgacgcc ggccgggtgc gcccgggcga ggtcatctgg tgcgagaacg gctcgtacgt
     2161 cgtggcgaag caccggttca aggaggaccg gctgccgttc ggcaacctga ccttcaccga
     2221 agtcctggcg cggtcttcga acgtgggcac gatcaaggtg gcgcagcgtc tgccgtcgac
     2281 ggacttcatc gaggtcatcc ggcgcttcgg cttcggccgc tccacctcgc tcgagctgcc
     2341 cggcgagagc cccggcctgc tgcgcgacct gaagcgctgg tccgggctgt cgcacgcttc
     2401 gatctccatg ggacaggaga tcggcgtgac cccgatccag ctcgccgcgg ccctcggcgc
     2461 cctggccaac gacgggatgt acaacccgcc ccacctcatc gaggcgttcg tcgcggccga
     2521 cggctcgcgc accgagccgg agcgggacgg cgacttcgag gcgcggcggg ccgtgtcggc
     2581 gaagaccgcc cggacgctgc gcgacatgct gcagtcggtg accatcgacg gcaccgggca
     2641 ggcggcgcag gtggtcggat acaccaccgg cggcaagacc ggcacggcgc agaagatcga
     2701 cccggccacg cgccgctacg cgcgcggcaa gtacgccgcc tggttcggcg gtttcgtgcc
     2761 ggccgagcgc ccggccctgg tgctcgtcgt gatggtggac gagccgaagg ggatccacca
     2821 gcacggcgga gacgtgtcgg ccccggtctt ctcgcgcgtt gccgagccgg tgctgcgcta
     2881 cctcggcgtc cttccggatc gcgaggaccc gctggtgatc gacgcgccgc tcgtcgccgc
     2941 ggcgcgcccg gccgccccgg ccgtgcagcc ggcgagtctg cgcacctcca gccggaagga
     3001 ggggagcgcc gtccccgcat ccaggacggc gccggccggc gccgcgccct cggacgcccg
     3061 gtcgttcggc cggaagatcc tcgacgcggc aacgcgcacc atcccggtgg cgggtggaac
     3121 ggtccaggcc ggcttcatcg ggagtcccgc cggcgccgac gaagggcgcg aggtggtgat
     3181 gccggacctc acgggcatga gcctgaagca ggcgaccgag gcgctggggc gcgtcgggct
     3241 gaattgcagc agccgtctca ccggcccgcg cgtctcgcgc caggagccgg tcgccgggct
     3301 cgccatcagg ccgggcgcac gctgttcgat cattctcgag taggggggtc attgcagttg
     3361 caggcagtcc tcagggacat cgcagggaat caggtctcgg gtgaccagac caccgcgatc
     3421 cgcgggatcg cgtacgactc gcgccaggtc gagccgggat tcctgttcgc ggcgctcgaa
     3481 ggcgaggcgc gcgacggcca cgacttcctc gatcaggcgc gcgcccgtgg agcggtcgcg
     3541 gtcctctcgg cgcgtccccg cgatccgcgc ttccccgggc tcgcctgggt gcaggtggag
     3601 gacgctcgcc gcgggctcgc cctcgcctcc cgcaactact acggccgccc cgacgagcgc
     3661 ctgatgatgc tcggcgtcac cggcaccaac ggcaagacga ccgtctgcca tctcctcgag
     3721 ggggggctcc gggaggccgg cctgcggccg tgcctgctcg ggaccgtgtc gtaccgctac
     3781 ggcaaggacg agacgcgcgc cgaccgcacc accccggagt cgctcgactt gcagcgccat
     3841 ctcgaccgct tcgccaccgc cggcgcccgc tcctgcgcca tggaggtgtc gtcgcacgcg
     3901 ctggccctgc ggcgcgtcgc cggcatcgcc taccgggcgg ccgtcttcac caacctgacg
     3961 caggaccacc tcgacttcca cagcaccatg gaggagtact tccaggcgaa ggcgctcctg
     4021 ttccgctcgc tcaagccgga cgccgtcgcg gtgctgaacg ccgacgatcc ggcggcggag
     4081 aggctggcgg cggtgaccgg cgcccgggtc gtcaccttcg gcgagtcggc gcgggtgcac
     4141 ctgcgtctct ccgaggtgcg ggcgaccctc gccgggaccg aggtgacgct cacgccgtcg
     4201 cgcggcctca ccgaccgggg cacggaggac ggcccgatcg ccgtgcgctc gccgctcctc
     4261 ggccggcaca acgcctcgaa cctggcggcc gccgcggcga cgcttctgag cgtcggggtt
     4321 ccggccgagg cggttccgaa ggggctcgcc gcggtgaccg gcgtgccggg gcggatggag
     4381 cgggtggacg atggcagcgc atttatcgtc ctcgtcgatt acgcccacac cgacgacgcg
     4441 ctcacccatc ttctcgagag cgtgcgcgcc ctgcagccga agcgggtcat caccgtcttc
     4501 ggctgcggcg gcgaccggga ccgggacaag cggccgaaga tgggcttcgc cgccgcgtcg
     4561 cacagcgaca tcgtcgtcgt gacctccgac aacccgcgca ccgaggaccc gcacgccatc
     4621 gtccgggcga tcctccccgg cgtggccagg gcactcggcg tcgaccccgg ggcgccgctt
     4681 cctgcggatc gctgccacgt gctggtggac cggcgcgagg cgatccaccg cgccgtgtcg
     4741 ctggcgcgcc cgggggacgc ggtggtcatc gccggcaagg ggcacgagcc gtaccagatc
     4801 ctcggcgagc gaacggtgcc gttcgacgac cggcaggtgg cgcgcgatgc cctgcacggc
     4861 ggcggtgcgg tgcgcggccg cgccgcctgc gagtgagatg gcgcggctga cgctcggcga
     4921 ggcggcgaag gcgatgtccg gacggatgag catcactgag cgggaacgcg ggcgggtggg
     4981 agagacgacg acggtgaccg gctacagcat cgattcgcgg accctcaagg cgggcgatct
     5041 cttcttcgcc atcgtcgggc cgcgcgtcga cggccacgac ttcgcccgcc aggcggcgga
     5101 gaagggggcg gcggcggtcg tggtgtcgaa gggcggcccg gggcgctacc cggaggcgcc
     5161 ggcggtgctg cgcgtcgacg acacgacgaa ggcgctgcag gatctcggcg gccacgtgcg
     5221 acgccgtcgc ccgttgaaag tcgtcggcat caccgggagc gcggggaaga ccaccgcgaa
     5281 ggagatgacc ggctcggtcc tcgaccggcg cttcaagacg caccgctccg aggggaacct
     5341 caacaacgcc tgggggctgc cgctgaccct cctccggatg caggacgacg tggaggccgc
     5401 cgtcctcgag atggggatgt cctacaaggg ggagatcgcc cgcctcgtcg agatcagcaa
     5461 cccggacgtc ggcgtcatcc tcaacgtcct gccggtgcac ctcgagcact tcgggtcgat
     5521 cgccggcatc gccgccgcca agggggagct gttccgcggc ctgcgacgcg acgccatcgc
     5581 cgtgtacaac gccgacgatg ctcgcgtcgc gcgtctcggc aaggcgttca aggggaccac
     5641 cctcccctac gggatcgcgg cgcggcgcgc cgaggtgaag gcggacgagg tcgtctccct
     5701 cggtctcaag ggaacccgct ttacgctgcg ctacggcgac atgcgcgccc cggtgcgcct
     5761 ggcgatcccg gggcggcacc acgtctacaa cgcgctcgcc gccgccgcca ccggcttcgc
     5821 ctgcggcctc gacccctccg acatcgccgc cgggctcgtc cggatgcgcc ccgccgcgat
     5881 gcgcggcgtg ctgcaccggc ggcaggacgg ggtcgagatc ctcgacgatt cctacaactc
     5941 caaccccgcg gcgatggagc gcgccatcga gctcctcgcc gagaccgcgc cgcgcggccg
     6001 ccgcatcctg gccgccggcg acatgctcga gctcgggccg gcttcggccc gggcgcacgc
     6061 ccggatcggg acgctcgcgg cccgcgccgg gatcgatctg ctggtcgccg tcgggccgct
     6121 gtcgaagcgg atggcccagg cggcggcccc caaagcgccg cgcaaagcag cggcgaccaa
     6181 ggtccgccac tgcgactcct cggaggaggc cgcgacctac ctcgcctccg agatccgccc
     6241 gggcgatctc gtcctcgtca agggatcgcg cggcatccgc atggagcgca tcgttcgggc
     6301 gctcctcggc gaaggcacca cgggagggac gcactgatgc tctactccct cctgtatccg
     6361 ctccacgaga cgttcagcat gttcaacgtc ttccgctaca tcacattccg gacggcgctg
     6421 gccaccctca cggcgctgtt gatcagcctt gccctgggtc cgcggctcat ccagaggctg
     6481 cggcacttcc agatcgggca gcagatcaga cccgagggac ctctttccca ccaaagcaag
     6541 aaaggtaccc cgacgatggg agggctgctg atccttatcg cagtcattct cccgactctc
     6601 ctctgggcag atcccgccaa tccctatgtg tggatcgcac tcatatccac actcctctac
     6661 ggagtcatcg gcttcgcgga tgactacctc aaactcacga gaaagaggtc tctcgggctg
     6721 acagcccggc agaagttcgc tgcacagatg gtcgtcgccc tcggcatcgg cctggtgctg
     6781 ctgtggatga cgaagcaggg gttctacagc acgcacctga gcctgccgtt cttcaagcgc
     6841 ttcgacccgg atctgggctg ggtcttcgtg ctgtggacca tgctggtggt ggtcggggcg
     6901 gcgaacgcgg tcaatctcac cgacggtctc gacggattgg cgatcggctc gtcgctgatc
     6961 gcctcgggga cgtacgccat cctcgcctac gtcgccgggc acgccaaggt gtcggcgtac
     7021 ctcgacgtcc ccaacgtgcg cggtgccggc gagctggcgg tcatctgcgg cgcgctcgtc
     7081 ggcgcgtcgc tcggcttcct gtggttcaac tgcaacccgg cgcaggtgtt catgggggac
     7141 gtcgggtcga tggcgatcgg cgggggctta ggcaccatcg ccatcctgat caagcaggag
     7201 gtgctgctga tcttcgtcgg cggcctcttc gtcatcgagg cgctgtcggt catcgcccag
     7261 gtcggttcgt tcaagctgcg cggcaaacgg gtcctgcgca tggcgccgat ccaccaccac
     7321 ttcgagctgg ccggctggcc ggagcagaag gtcgtgatcc gcttctggat cgtcgccctc
     7381 atcttcgccc tgctgggtct gtccactctc aagctgcgat gagggcccga tgatgccggc
     7441 tccccgtccg ccccgcaccg cgcccgacct ccgtggcgcg cgcgtcgtcg tggccggcgc
     7501 cgcgaagagc ggcgtggcgc tcgcccggtt cctcctggcg cgcggcgcct ccgtcaccct
     7561 gagcgaccag cgtcaggcgc gcgacctcgg cccggaggtg gcggcgctcg cggcgcgggg
     7621 ggtcgcgttc gagttcgggc ggcacgagga ggccaccttt tccggggccg acctcgtggt
     7681 ggtgagcccg ggcgtgccgc tgtcgatcca gcctctcgcc gccgcccgcc ggcgcggcgt
     7741 gcgcgtggtc gcggaggtcg aggtggcgtc ctggtatctc gaggggaccc tcatcgggat
     7801 caccggctcg aacggcaaga gcaccacgac cgccctcgcg gggcacctgc tggcccgggc
     7861 cggcagaaac gccgtcgcct gcggcaacct cgggacgccg ctcatcgaga cgctcgagca
     7921 ggacgcgccg gaccgcctgt acgtcgtcga gctgtcctcc ttccagctcg aggggatcga
     7981 aacgttccgg ccgcacgtct cggtcctgct caacctgagc cccgaccacc aggaccgcta
     8041 ccccgacacg gcggcgtact acgccgccaa ggggcgcctg ttcatgaacc agaaagcggc
     8101 cgatcacgcc gtgctgaacc aggacgacga ggcggtggcc ctcctcgcgc gcggcatcgc
     8161 cccgcgccgg catggcttca gccgcgcggg aagggtcgcc gacggcgcct acgtccgcgg
     8221 cgccgacatc ctcctcgtcg aggacggtcg cgagcagccg gtcctgccgg tcaaggacct
     8281 cccgctcttc ggcgtgcaca accaggagaa cgtcatggcg gcgctgctcg tggcgcgcct
     8341 ctgcggcgtc gcggcggagc aggcggcgga cggggtgcga tcgttccgcg gcctgccgca
     8401 ccgcctcgag cgggtgcgcg acctcggcgg cgtgtcctgg ttcaacgact cgaaggcgac
     8461 caatgtcggc gcggcggcgc agtccctgcg ctcgttcgcg ggcggggtgg tgctcatcat
     8521 gggggggaag gacaagggcg ggaccttcgc cgacctcacg tcgctcgtcc gcgaccgggt
     8581 gacccacctc gtcctgctgg ggaaggcgaa ggagacgatc gcccggcaga tcgggccgat
     8641 cgtgccgacg atcctggtcg aggacatgga ggacgcggta gccgccgccc gcgtggccgc
     8701 cctccccgga ggggtggtgc tcctcgcccc cggctgcgcc tcgttcgacc agtactccgg
     8761 cttcgaggcg cgcggcgagc atttcaggag cctcgtgaac gcgctcgagg agacgtaggg
     8821 atggcgcgca aactgacgtt cgaccggccg ctgttcgtcg tcggcatcgg gctggcgctc
     8881 ttcggcctgg tgatgatctt cagcgcctcc gcccccctgg cgatggagcg acaccagacg
     8941 ccgtaccact tcctgatgcg gcagatgctg gcgttcgcga tcggcctctg cgggatggcg
     9001 gtggcgatga acttcgacta ccgggccctt ctcaaacgcc ccgcggtcgg tctcgcgatc
     9061 ggcggcgcgg cgctgctcct cgcgggggtg ctggtcgtcg atcggaccca ccaggtgcac
     9121 cgctggttca aggtcggccc gatccaggtg cagccgtcgg agatcgcgaa gatcgcgctg
     9181 gtgatgttcc tggcggcgca gatggcgaag cacgagaacc gggtgaacga catcgcccgg
     9241 tcgctcctgc ccaccgccgt cgtcgtggtg ccgctcgccg ggctcgtcta cctcgagccg
     9301 gacctcggca ccgcg
//
LOCUS       DASRIQ010000069         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1585922, whole genome shotgun sequence.
ACCESSION   DASRIQ010000069 DASRIQ010000000
VERSION     DASRIQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585922"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /locus_tag="VFD06_01790"
     CDS             <1..1911
                     /locus_tag="VFD06_01790"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZN02296.1"
                     /translation="DRLIPIVADPFVDSTFGTGAVKVTPAHDPNDFAMAERHDLPRLR
                     VMDDQARMNDAVPAPYRGLDRFEARQRVVDDLHAAGFLRGVKDHPHNVGHCQRCGTLA
                     EPFLSKQWFVRIGPLAEPATRAVAEGRVAFTPAHWVNSYNEWMGRIHDWCISRQLWWG
                     HPIPAWHCAACGLVTVARQDPGTCPGCRAARTVADPDVLDTWFSSALWPFSTLGWPSV
                     TPDLARYYPTTTLVTGFDILFFWVARMIMMGLRFMNEVPFHRVYITGLVRDAEGQKMS
                     KTKGNVVDPLELVERYGADALRFTLAAMATPGSDLPLSEERMAGYRAFANKLWNAARF
                     VLLNAGPNATGTAPPLPPAALRTLPDRWILSRLAGLRVEVDRALGEFRFDEMANALYR
                     FLWHEYCDWYIEAAKPSLAPGAPEEDAARARAVLLHVLEEVLRLLHPVMPFLTEEIWQ
                     RLPHHGETIAAAPYPSPDGAEAARDPDAERRMDMLMEIVGRVRNLRAEVNVDPGRKVP
                     LLVRPHDEEGRRLLTTQAALLTSLARLESLRVTDDLAAAGTAARAVAAGADLALPLTG
                     ILDVDAERRRLEREIERLTKERVGHASKLENADFLARAKPEVVEKVRGIDREIGERLD
                     RLAATLRSLRGA"
     gene            1925..2776
                     /gene="nadC"
                     /locus_tag="VFD06_01795"
     CDS             1925..2776
                     /gene="nadC"
                     /locus_tag="VFD06_01795"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZN02297.1"
                     /translation="MLDLEAAGRSVRRALEEDVGEGDVTSRAVVPEDARALGRFVARE
                     PLVLAGLPVAREVFRRVDASLLFEPLAEEGSLCAEGAILAIVRGPARPLLAGERTALN
                     FLQRLSGIATATRALVDRVRGSRLQISDTRKTAPGLRALDKYAVEVGGGTNHRFGLYD
                     AWLIKDNHWRLAGGVGPALHRARAALGGRPAPGIGLEIEVGTVDEVREAIEAGADALL
                     LDNMDDATLRQAVEAARGRAFLEVSGNVGAERLPALAQLGVDRVSIGALTHSVRAVDV
                     ALEVEAA"
BASE COUNT          382 a         1085 c         1056 g          399 t
ORIGIN      
        1 gaccggctca tcccgatcgt cgccgacccc ttcgtggaca gcaccttcgg gaccggcgcc
       61 gtgaaggtca cgccggcgca cgacccgaac gacttcgcga tggcggagcg gcacgacctc
      121 ccccggctcc gggtgatgga cgaccaggcg cggatgaacg acgccgtccc cgccccgtac
      181 cgcggtctcg atcgcttcga ggcgcgccag cgcgtcgtcg acgacctgca tgcggccggc
      241 ttcctgcgcg gggtgaagga ccacccgcac aacgtcggcc actgccagcg ctgcggcact
      301 ctcgccgagc cgttcctgtc gaagcagtgg ttcgtgcgca tcggaccgct ggccgagccg
      361 gcgacgcgcg ccgtggccga ggggcgcgtg gccttcacgc cggcccactg ggtgaactcc
      421 tacaacgagt ggatggggcg catccacgac tggtgcatca gccgccagct ctggtggggg
      481 cacccgatcc cggcgtggca ctgcgccgcc tgcggtctcg tcaccgtggc gcgccaggat
      541 cccggcacct gccccgggtg ccgggccgcc aggacggtcg ccgatcccga cgtgctcgac
      601 acctggttct cctcggccct ctggccgttc tcgaccctcg gctggccatc ggtgacgccc
      661 gacctggccc gctactatcc gacgacgacg ctggtcaccg ggttcgacat cctgttcttc
      721 tgggtggcgc ggatgatcat gatggggctc cggttcatga acgaggtccc gttccaccgc
      781 gtctacatca ccggtctcgt gcgcgacgcc gaggggcaga agatgtcgaa gaccaagggg
      841 aacgtcgtcg atcccctcga gctcgtggag cgctacggcg ccgacgcgct gcgcttcacc
      901 ctcgccgcca tggcgacgcc ggggtccgac ctccccctct ccgaagagcg gatggccggc
      961 taccgcgcct tcgccaacaa gctgtggaac gccgcccgct tcgtcctgct caacgccggg
     1021 ccgaacgcga cggggacggc gccgccgctg ccgcccgcgg cgctccggac gctcccggac
     1081 cgctggatcc tgagccgcct ggccgggctc cgcgtcgagg tggatcgcgc gctcggagag
     1141 ttccggttcg acgagatggc gaacgcgctg taccggttcc tgtggcacga gtactgcgac
     1201 tggtacatcg aggcggcgaa gccgtccctc gcccccgggg ctcccgaaga ggacgccgcg
     1261 cgggcccgcg ccgtgctcct gcacgtcctc gaggaggtgc tgcgcctgct gcaccccgtc
     1321 atgccgttcc tgaccgagga gatctggcag cgcctgccgc accacggcga gacgattgcc
     1381 gccgccccct atccgtcgcc ggacggggcg gaggcggcgc gcgatccgga cgcggagcgc
     1441 cgcatggaca tgctcatgga gatcgtcggc cgcgtgcgca acctccgcgc ggaggtgaac
     1501 gtcgaccccg ggaggaaggt gccgctcctg gtgcggccgc acgacgagga ggggcgacgc
     1561 ctgctcacaa cccaggccgc gctcctgacg tcgttggccc gcctcgaatc gctgcgcgtc
     1621 acggacgacc tcgccgccgc cgggacggcg gcgcgggcgg tcgccgcggg agccgacctg
     1681 gcgcttcccc tcaccgggat cctcgacgtc gacgccgagc gccgccgcct cgagcgggag
     1741 atcgaacggc tgacgaagga gcgggtcggg cacgcgtcga agctcgagaa cgccgacttt
     1801 ctggcccgcg ccaagccgga ggtagtggag aaggtgcgcg gcatcgaccg ggagatcggc
     1861 gaacggctcg accgcctggc cgcgaccttg cggtcgcttc ggggagcgta ggagaatcgc
     1921 cgccatgctc gatctcgagg cggccggccg ctccgtgcgt cgcgccctgg aggaggacgt
     1981 cggcgaggga gacgtcacct cccgcgccgt cgtcccggag gacgcgcgcg cgctcggacg
     2041 cttcgtggcc cgcgagccgc tcgtcctcgc cgggctgccg gtggcgcgcg aggtgttccg
     2101 ccgggtcgac gcgtcgctcc tcttcgagcc gctggccgag gaggggagcc tctgcgccga
     2161 aggcgcgatc ctcgcgatcg tgcgcggccc cgcccggccg ctcctggccg gggagcggac
     2221 ggcgctcaat ttcctgcagc gcctctccgg catcgccacc gccacccgcg cgctcgtgga
     2281 tcgggtgcgc ggttcgcgcc tgcagatctc cgacacgcgc aagaccgccc cgggcctgcg
     2341 ggcgctcgac aagtacgccg tggaggtcgg aggggggacg aaccaccgct ttggcctcta
     2401 cgacgcctgg ctcatcaaag acaaccactg gcggctggcg ggcggggtcg gcccggcgct
     2461 gcaccgggcg cgggcggccc tcggtgggcg gccggccccc gggatcggcc tggagatcga
     2521 ggtcggcacc gtggacgagg tgcgcgaggc gatcgaggcg ggggcggacg cgctcctcct
     2581 cgacaatatg gacgacgcga cgctgaggca ggccgtcgag gcggcgcggg ggcgcgcctt
     2641 tctcgaggtc tcgggcaacg tcggcgccga gcggctgccg gcgctggcgc agctcggcgt
     2701 cgatcgggtc tcgatcgggg ccctcaccca ctcggtgcgc gccgtcgacg tggcgctcga
     2761 ggtcgaagcc gcgtgagcga cgacacgcct cccgcggacg cgctcacccc ggcggccgtg
     2821 cagcggcacc tgcgcgccgc cgtcttcggc catcgcatct tctactaccc gagcatcggc
     2881 tccaccaacg agcgggcgct cgagctcgcg gccgccggcg ag
//
LOCUS       DASRIQ010000070         4301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1079621, whole genome shotgun sequence.
ACCESSION   DASRIQ010000070 DASRIQ010000000
VERSION     DASRIQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079621"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFD06_01800"
     CDS             complement(<1..768)
                     /locus_tag="VFD06_01800"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZN02298.1"
                     /translation="MPDPGAAGLVIRGAGVSDVGKKRRSNEDAYSTSDDLRLYVVADG
                     MGGHAAGEVASRLAVESIGRHISSAQPDVPPTQPTEASTRSLEETSLPVPARRVLNAI
                     RLANQEIVRSVRRDQSMRGMGTTVVMVLIQGRRAYIGSVGDSRAYLVRDGDIRQLTDD
                     HTFVNEQVRSGTLTAQEARRHPARNILTRAVGSQEDIEADLIEEDLKPGDLLLLCSDG
                     LTNMVEDQEILETLRRDGVDPEAGCQALIELANERGGD"
     gene            complement(761..1726)
                     /locus_tag="VFD06_01805"
     CDS             complement(761..1726)
                     /locus_tag="VFD06_01805"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02299.1"
                     /translation="MRFLKAIRNLLGNYQLKSGIYHHDRGESQQAIDFLMRALQSPES
                     TEPDRRMALYYLTQAYIALAEKLEEGDEMDKAVEAYRQALAITPDYPDLHFRLGALCG
                     RLGLSTDAIEAFGRALELNPDYLEARVRRAMLLLERRDTAAAAAEFAAVRDVAVRAID
                     QPFEQGRQCLDQQDLEGAAEWMRTAFLRRPESFEFHFRRGLRSLRDGKMEPAIEALRL
                     ATQFRSDFADVHNYLGVACAEQQDWDAAVAAFRKALEVNEDYVVARLNLAFALAEAGR
                     EEEAADELRQVLRQEPDNQPALARLEELSAPKREPRTRSQGESRA"
     gene            complement(1726..2871)
                     /locus_tag="VFD06_01810"
     CDS             complement(1726..2871)
                     /locus_tag="VFD06_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02300.1"
                     /translation="MGAAEPLPATPRRHPLFERVLQTNAPEALRLGAARGALPLPLHD
                     LVALQVRLQRDPSKAVATAAVESLGRLPDESLIAFLSDPGCDVALIDFAARSGRLKGE
                     GLAAAIFHPAVGDTTLEALATEGDAEALGLVLINEMRLIRNTRLLAILRANPRFTGEQ
                     RRRLAELEREVIGREPRLVAVPKVVPDAPAVEASAVPDATEALPADPLPPPNDAELPP
                     EGAADPNQEYFDEEALQKTDAYQKIQRLNVAERNLLAMKGNAEERAILVRDTARVVSH
                     AVLKNPRLSETEIVRFAGMRSIHEDVLRAIASNREWTKGYPVALALVRNPKTPAGLSV
                     QFLARLGTRDLKICAGDKNIPEMVRRQARNLFIVRTQPPKKTFKKAH"
     gene            complement(2858..4090)
                     /locus_tag="VFD06_01815"
     CDS             complement(2858..4090)
                     /locus_tag="VFD06_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN02301.1"
                     /translation="MANFGESLKRERELRDIPLRDIANATKINLRYLEALEANRFDRL
                     PGGVFNKGFIRAYARFIGADGEALVQRYMEEMSEREAAVASAQPTPDRPGLIRPAQGP
                     PPRRADPAGGAGAAPAEGSPSITFVEGRAGSAAVAAMAAARRSGRTAPTGSADSFNPS
                     ATGPQARLILAATTVVAAFAALAIVLYLVRSARHDARVPATSHEGAPASAPEAGGAGD
                     PTAVPPQDSEAQEDGAAPDAPAAPPAGTDSEPSPTAPPQRTLPEVPHDRDGKTSPVRV
                     ASPIPTPVPETAKPVPHAAAPPPPQPPTAGADNAPPSPAGPMTLVVQGVNPVRVQLGC
                     DGVDRPVRMLVAGESVTLRCYSLVRISTEDAGAVRLTLDGAACPPLGAAGSRVEHYTI
                     RAEDARAICPPGGSRGRR"
BASE COUNT          555 a         1483 c         1615 g          648 t
ORIGIN      
        1 gtccccgccc cgctcgttgg cgagctcgat gagggcctgg cagccggcct cggggtcgac
       61 gccgtcgcga cgcagcgtct cgaggatctc ctggtcctcc accatgttgg tgagaccgtc
      121 ggaacagagg agcaggaggt cgccgggctt caggtcctct tcgatcagat ccgcctcgat
      181 gtcctcctgg ctgccgacgg cgcgggtcag gatgttgcgg gccgggtggc ggcgcgcctc
      241 ctgcgcggtc agcgtgccgg agcggacctg ctcgttgacg aaggtgtggt cgtcggtgag
      301 ctggcggatg tcgccgtcgc gcaccaggta cgcccggctg tcgccgaccg agccgatgta
      361 ggcgcggcgg ccctgaatga ggaccatcac cacggtcgtc cccatgccgc gcatgctctg
      421 gtcgcggcgc accgatcgga cgatctcctg gttcgcgagg cggatggcgt tcagcacccg
      481 gcgcgccggg accggcaggg aggtctcctc gagcgatcgc gtcgaggcct cggtcggctg
      541 ggtcggcggc acgtccggct gggccgagga gatgtggcgg ccgatcgact cgaccgcgag
      601 ccgcgaggcg acttctcccg cggcgtgccc ccccatgccg tcggcgacga cgtagaggcg
      661 cagatcgtcc gaggtgctgt aggcgtcctc gttggagcgg cgcttctttc cgacgtcgga
      721 gacgccggcg ccacggatga cgagtcccgc ggcccccgga tcaggcacgc gattccccct
      781 gcgagcgggt ccgcggctcg cgcttcggcg ccgagagctc ctcgagacgg gcgagcgccg
      841 gctggttgtc gggctcctgg cgcagcacct ggcgcagttc gtcggccgcc tcctcctcgc
      901 gccctgcctc ggcgagcgcg aaggcgaggt tcaggcgggc gaccacgtag tcctcgttca
      961 cctcgagggc cttgcggaag gcggcgacgg cggcatccca gtcctgctgc tcggcgcagg
     1021 cgacgccgag gtagttgtgc acgtcggcga aatcggagcg gaactgcgtc gccaggcgga
     1081 gcgcctcgat cgccggctcc atcttgccgt cgcgcaggct ccggagcccc cgccggaaat
     1141 ggaactcgaa cgactccggg cggcgcagga acgcggtgcg catccattcg gccgccccct
     1201 cgaggtcctg ctggtcgagg cactgccgcc cctgctcgaa cggctggtcg atggcgcgga
     1261 ccgccacgtc gcgcaccgcc gcgaactcgg ccgccgccgc ggccgtgtcg cgccgctcga
     1321 ggaggagcat ggcgcggcgc acccgggcct cgagatagtc ggggttgagc tccagggcgc
     1381 ggccgaaggc ctcgatggcg tcggtcgaca gcccgaggcg gccgcacagc gcgccgaggc
     1441 ggaagtggag gtccgggtag tccggcgtga tcgctagcgc ctgccggtac gcttccacgg
     1501 ccttgtccat ctcgtcccct tcctccagtt tctcggccag ggcgatgtac gcctgggtca
     1561 gataatacag cgccatgcgc cgatccggct ccgtcgactc gggggactgc agcgcgcgca
     1621 tcagaaagtc gatcgcctgc tgcgactcgc cccggtcgtg gtggtagatg ccggacttca
     1681 gctggtagtt gccgagcagg ttgcggatcg ctttgagaaa gcgcatcagt gggccttctt
     1741 gaaggttttc ttcgggggct gggtgcgcac gatgaacagg ttgcgggcct gccggcggac
     1801 catctccggg atgttcttgt cgccggcgca gatcttgagg tcgcgggtgc cgagccgggc
     1861 gaggaactgc accgacaggc cggccggcgt cttcgggttg cgcaccagcg ccagcgccac
     1921 ggggtacccc ttggtccact cccggttcga ggcgatggcg cgcaggacgt cctcgtggat
     1981 gctgcgcatc ccggcgaagc ggacgatctc ggtctcggag aggcgcgggt tcttcagcac
     2041 cgcatgcgag acgacccgcg cggtgtcgcg gacgaggatc gcccgttcct cggcgttccc
     2101 cttcatggcg aggaggttcc gctcggcgac gttgagacgc tggatcttct ggtaggcgtc
     2161 ggtcttctgc agcgcctcct cgtcgaagta ctcctggttc gggtccgccg ccccctccgg
     2221 cggaagctcg gcgtcgttcg gggggggaag ggggtctgcg ggaagggcct ccgtcgcgtc
     2281 gggaaccgcg gaggcctcca ccgccggcgc gtccggaacg accttcggga ccgccacgag
     2341 ccgcggctcg cgcccgatga cctcgcgttc gagctcggcc aggcggcgtc gctgctcgcc
     2401 cgtgaagcgc ggattggcgc gcaggattgc gagcaaccgc gtgttgcgga tgagccgcat
     2461 ctcgttgatg aggacgaggc cgagggcctc ggcgtccccc tcggtcgcga gcgcctcgag
     2521 ggtcgtgtcg ccgaccgccg ggtggaagat cgccgccgcc agtccttccc ccttgagccg
     2581 ccccgaacgc gctgcgaaat cgatgagcgc cacgtcgcac ccgggatcgc tgaggaacgc
     2641 gatcaggctc tcgtccggca gacgtccgag gctctcgacg gccgcggtcg cgaccgcctt
     2701 cgacgggtcc cgttgcaggc gcacctgcaa cgccacgagg tcgtggagcg ggagcggcag
     2761 cgcgccgcgc gcggcgccga gccgcagcgc ctccggggca ttcgtctgca gcaccctctc
     2821 gaagagcggg tggcgccggg gcgtcgccgg gagcggctca gcggcgccca cgggatcctc
     2881 cggggggaca gatggcgcgc gcgtcctcgg cgcggatggt gtagtgctcg acccggctgc
     2941 cggcggcccc gagcggcggg caggcggcgc cgtcgagcgt gaggcgcacc gccccggcgt
     3001 cctcggtgct gatccgcacg aggctgtagc agcgcagagt caccgactcg ccggcgacca
     3061 gcatccggac cggtcgatcc acgccgtcgc agccgagctg caccctcacc gggttcaccc
     3121 cctgcaccac gagtgtcatc gggccggcgg gagacggggg agcgttgtcg gcgccggcgg
     3181 tgggcggctg cggcggcggc ggagcggcgg cgtgcggcac cggctttgcc gtctcgggga
     3241 ccggagtcgg gatgggggac gcaacccgca ccggcgacgt cttcccgtcg cgatcgtgcg
     3301 ggacctccgg aagcgtgcgc tgcggcggag cggtcgggga gggctcactg tcggtccccg
     3361 ccggcggcgc cgcgggcgcg tccggtgcgg cgccgtcttc ctgcgcctcg gaatcctgtg
     3421 gcggaacggc cgtcgggtcc cccgcgccgc ccgcctccgg cgccgacgcc ggcgcgccct
     3481 cgtgcgacgt ggccggaacg cgcgcatcgt gacgcgccga tcggaccagg tacaggacga
     3541 tggccagggc cgcgaaggcc gcgacgaccg tcgtcgccgc gaggatcagg cgcgcttgcg
     3601 gacctgtggc cgaagggttg aaggagtcgg ccgatcccgt cggcgccgtc cggccggagc
     3661 ggcgcgcagc ggccatggcg gcgacggcgg cggacccggc gcgcccttcc acgaaggtga
     3721 tcgaagggga gccctcggcc ggcgccgccc cggcgccgcc ggccggatcg gcgcggcgcg
     3781 gcggcggccc ctgcgccggg cgtatcagcc cgggacgatc cggagtcggc tgcgccgaag
     3841 cgacggctgc ctcgcgctcc gacatctcct ccatgtagcg ctgcaccagc gcttccccgt
     3901 cggcgccgat gaagcgggcg tacgcccgga tgaagccctt gttgaagacg cccccgggga
     3961 gccggtcgaa gcggttcgct tcgagggcct cgagatagcg caggttgatc ttggtggcgt
     4021 tggcgatgtc gcgcagtgga atgtcgcgca gctcgcgctc gcgcttcagg gattcgccga
     4081 aattcgccat gcggccgtct gctccgcccg aaaacgtgcg cagaccgctt tggggacgga
     4141 caatcgcttt ccgccggcga cggcgcgcgg gggggaataa taaggagggg tgccgggttt
     4201 gtcaaaggca attaaatctt taccgttctg taatttaggc agaaaaacgg cgcctgttcg
     4261 cgtcctagga gcccgtccga gtcttggcat gggccgcgcg c
//
LOCUS       DASRIQ010000071         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2107679, whole genome shotgun sequence.
ACCESSION   DASRIQ010000071 DASRIQ010000000
VERSION     DASRIQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107679"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VFD06_01820"
     CDS             <1..794
                     /locus_tag="VFD06_01820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN02302.1"
                     /translation="VGAASLIVQSLAPAWTVPVVAGASVAAVLLVLFDLYRRLLGMLH
                     ESHHAPGQNYRQIEALFSLFSLVRVEHPLPAMRGPAVSPDFACDLVSLIREIRPACVL
                     EAGSGVSTLLAGYALKASGRGRLISIEEDAGFAEITRRRVADHGLQSCVTVVHAPLKT
                     VRAGAHEHLWYDTEVLKDLPEVDLFIVDGPRQENLPARHLRYPALPLLFPRLSRGAAV
                     LVDDCHRADEREIVERWLREFTGFEAERTWGEKRHTILRRRHQVS"
     gene            complement(807..>2667)
                     /locus_tag="VFD06_01825"
     CDS             complement(807..>2667)
                     /locus_tag="VFD06_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02303.1"
                     /translation="LGMPGVWTWNFREAFGHHYLDSVAMNHNSLGRGYETWGNATAET
                     VRRVLDRSDTTMEWYRPLPPPAEFAWSARDNLNYTQTALLAALDQVAGDPAAMLRDFH
                     RKGWNSWRKGVEEAPFGFIIPEDQGDRARVAQMIGRLLDQKIEVARATAPLVLKEGRF
                     PAGTYVVRLDQPYRNYAVDLLTPQRFPKDGGEPYDDVSWSLPAHDHLEAIPTADPAVR
                     AAATTPLDAMPRPRGRVSGNGPVLLLADTGQEGLLAARYRLARFRVDVAGRAFKHGGA
                     SYPAGSWIVPAQDGVRDAARAVAEDLGLDFTSTATVPDVPRREAPAPRLGVWVPWADT
                     DSIGWVRYALDRRRAPYTYLRDEDVRAGNLRDRVDVILYGHVDLELAEQIEGLPKRWS
                     PLPYTKTAATPSHGVPAASEDITGGIGYEGMAQLQRFVETGGLLVTLGNGSMLALEAG
                     LVRGVRRAAGGVPRSSQGSGEAEAAASTGGATRTPGAHVRVTVARPGHPIVYGYPERT
                     HVFRQNYALYDTPRSWLRMAYCTSCLDGPEDASGVVMEWGDRQGAPLVVSGQAWGESG
                     LVGRPAILDLPVGRGHVVAFNFNPLHRDLNHGDHRLVWNAILNWQAIVAGPGR"
BASE COUNT          371 a          964 c          921 g          411 t
ORIGIN      
        1 ccgtgggcgc cgcgagcctg atcgtccaga gcctggcgcc ggcctggacg gtgccggtgg
       61 tcgcgggcgc ttcggtggcg gccgtcctcc tggtcctctt tgatctctac cgccgccttc
      121 tgggaatgct ccatgagtcc catcacgcgc cgggccagaa ctacaggcag atcgaggcgc
      181 tgttctcgct tttctcgctg gtccgcgtcg aacaccctct cccggcgatg cgcggtcccg
      241 ccgtctctcc ggatttcgcg tgcgatcttg tctctctgat tcgcgagatc cgcccagcgt
      301 gcgtcctgga ggcgggatcg ggagtgtcga cgctgctggc ggggtacgcg ctcaaagcct
      361 caggacgggg ccgcctgatc tccatcgagg aggacgcggg attcgccgag atcacgcgcc
      421 ggcgcgtcgc cgatcatggt ctccagtcct gcgtcaccgt cgtgcacgcg ccgctcaaga
      481 ccgtccgggc cggggcgcac gagcatctgt ggtacgacac ggaggtcttg aaggacctcc
      541 ccgaggtcga cctgttcatc gtcgacggtc cgcgtcagga gaacctccca gcgcggcacc
      601 ttcgctatcc cgccctgccg ctgctcttcc cgagactgag ccgcggcgcc gccgtcctcg
      661 tggacgattg tcaccgcgcc gacgaacggg aaatcgtgga gcgatggttg cgggagttca
      721 ccgggttcga ggcggagcgc acctggggcg aaaaacgaca caccatcctg cgccggcggc
      781 accaggtgtc ctgactggtt cgcgagtcac cgccccggtc cggcgacgat cgcctgccaa
      841 ttcaggatgg cgttccagac gaggcgatgg tcgccgtggt tgagatcgcg gtgcagcggg
      901 ttgaagttga acgccacgac gtgcccgcgg ccaaccggca gatcgagaat cgccggccgg
      961 ccgacgagac cgctctcgcc ccacgcctgg ccactgacga cgagcggcgc cccctgacgg
     1021 tcgccccatt ccatgaccac gcctgacgcg tcctccgggc cgtcgaggca ggacgtgcag
     1081 taggccatcc gcagccagga gcgtggggtg tcgtagaggg cgtaattctg ccggaagaca
     1141 tgcgtccgtt ccgggtagcc atagacgatc ggatgccccg ggcgggcgac ggtgacccgc
     1201 acgtgcgccc ccggcgtgcg ggtcgcgccc cccgtggacg ctgcggcctc ggcctcgccg
     1261 cttccctggc tgctgcgcgg cacgccgccc gcggcgcgcc ggaccccgcg caccagcccc
     1321 gcctccagcg ccagcatcga tccgttgccg agggtcacga gcagcccgcc cgtctcgacg
     1381 aaacgctgca gctgcgccat cccttcgtag ccgatgccgc cggtgatgtc ctcggacgcg
     1441 gccgggacgc catggctcgg cgtcgccgcc gtcttcgtgt agggcagcgg cgaccaccgc
     1501 ttcggcaatc cctcgatctg ctccgccagc tcgaggtcga cgtggccgta gaggatcaca
     1561 tcgacccggt cgcgcaggtt cccggcgcgc acgtcctcgt cgcgcaggta ggtgtagggg
     1621 gcccgccgcc ggtcgagcgc gtagcgcacc catccgatcg agtcggtgtc ggcccacggc
     1681 acccagacac cgagtcgtgg ggccggcgcc tcgcgccggg gcacgtccgg aaccgtggcc
     1741 gtgctcgtga agtcgagccc cagatcctcg gccaccgcac gcgcggcgtc gcgcacgccg
     1801 tcctgcgccg gcacaatcca cgatccggca ggataggacg cgccgccatg cttgaaggcg
     1861 cgtccggcga cgtcgacccg gaaccgggcg aggcggtagc gcgccgccag aagcccttcc
     1921 tggccggtgt cggcgaggag aagcaccggc ccgttgccgc tcacgcgccc gcgcgggcgt
     1981 ggcatcgcgt cgagcggcgt cgtcgccgcg gcgcgcacgg cggggtcggc ggtcgggatc
     2041 gcctcgaggt gatcgtgcgc cggcagcgac caggagacgt cgtcgtaggg ctcgccgcca
     2101 tccttcggga agcgctgcgg ggtcaggaga tcgacggcgt agttgcggta cggctgatcc
     2161 agccgcacga cgtaggtccc ggcgggaaag cggccttcct tgaggaccag cggtgccgtg
     2221 gctcgggcca cctcgatctt ctggtcgagc aggcggccga tcatctgcgc cacccgcgcg
     2281 cggtccccct ggtcctccgg gatgatgaag ccgaacggcg cctcctcgac ccccttgcgc
     2341 cacgagttcc atcccttgcg atggaagtcg cgcagcatcg cggccggatc ccccgccacc
     2401 tggtcgagcg ccgccaggag cgccgtctgg gtgtagttga gattgtcgcg cgccgaccag
     2461 gcaaactccg ccggcggcgg cagcgggcgg taccactcca tcgtggtgtc ggatcgatcg
     2521 aggacccggc gcaccgtctc ggcggtggca ttgccccacg tctcgtaccc gcggccgagg
     2581 ctgttatggt tcatcgccac cgaatcgaga tagtggtggc cgaaggcctc gcggaagttc
     2641 caggtccaca cccccggcat ccccagg
//
LOCUS       DASRIQ010000072         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_283179, whole genome shotgun sequence.
ACCESSION   DASRIQ010000072 DASRIQ010000000
VERSION     DASRIQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283179"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFD06_01830"
     CDS             complement(<1..435)
                     /locus_tag="VFD06_01830"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HZN02304.1"
                     /translation="MSRRSPAGLVPGIALLLLTAACGYHLSGHGSTLPPTVKSVGIPM
                     FENKTSRPELEQRVTEHVVDEFTSRGRVRILPGEEGADAVLIGTLFTYTTTPVILNEQ
                     GRARRYEILITARVVLSQAATDTILWEDDHFLFKRQYDVPETS"
     gene            complement(492..3002)
                     /gene="leuS"
                     /locus_tag="VFD06_01835"
     CDS             complement(492..3002)
                     /gene="leuS"
                     /locus_tag="VFD06_01835"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZN02305.1"
                     /translation="MNGDYRDQFDALERSWQERWERERLWSAPQRPTGRKYYCLEMLP
                     YPSGRLHMGHVRNYSIGDAVARFRRMRGEAVLHPMGWDSFGMPAENAAIQRGIPPAKW
                     TTDNIASMRAQLRRLGFGYDWGREIAAHRPEYYRWNQWFFLRMLEKDLAYRSRREVNW
                     CDSCQTVLANEQVEGGRCWRCDSVVGRRELDQWFLRISRYSEELVAGLAELPAWPEAV
                     RSIQENWIGRSEGAIVRFPLAGAGAAGSDAIEIFTTRLDTICGATFCVVAPEHSILRA
                     VPAGSTEARAIEELRSTAAPRREATAGETEKRGVDTGLRVTNPYSGEAIPVWAGNFVL
                     MGYGTGAIMAVPGHDERDFEFAQQYGLPIRVVVRPGPGSTAPSADEHGLPSAAMTDDG
                     VLVNSGPYDGLDSATARARMLADGEKKGQARTSVQYRLLDWGISRQRYWGTPIPVIYC
                     ERDGVVPVPDADLPVILPEDIPLTGTGGSPLAQSAAFVATTCPRCGGKARRETDTMDT
                     FIDSSWYFYRYCDARNPKQPFDPAIVAAWFPIDLYIGGINQAHLHLIYTRFFTRVLRD
                     LGLVRFSEPVVQMMCQGMVLKGGTAMSKSKGNIVDPDELIRRYGADTTRLFTLFAAPP
                     EKDLEWDDQGVEGCFRFLERIWRLFAPRAALLAAAPLPGPKDGADEPRRAALRRKVHR
                     TIQRVTHDLDRRLHLNTPVSSLMELLNETTDVARQERAGDEPYLKEAGRALALLLQPL
                     APHVSEALWEGMGGGTSVLLEPWPEADPAWLVEDEVELVVQVNGRLRGHVRVAADAPE
                     ADALARARVDPRVGAHLEGKSVRKTIYLPGRLLNIVVQ"
     gene            3225..4331
                     /locus_tag="VFD06_01840"
     CDS             3225..4331
                     /locus_tag="VFD06_01840"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZN02306.1"
                     /translation="MPADDPVLDPVALLASPGPAERVASALLQDLDPGSIPVLLDRLV
                     FHRVDGLAWRTLSSLAPGTVDEWLRATLRRRHQARSAATLAQGLALAEILEAWHRAGI
                     PAAVQRGLRVVDWIYKDAGARPFEDHDLLIRPADAEGAASLLRRLGYEPIGPALFRRA
                     TVLVDLHTDPLGSARRPSRGRIFPFDVDALFAAARPGYVAGGPALLLRSEDDLVLMAL
                     HVVKHSFDRLIRSADLAHLLAFEGRALSWERVRETADRAHATRLVTLALAALTPFGIT
                     PPEPFRLEEPVTGLSGLLLQRVRALRPLPYGGEILMALAAPRFADRLRFLFDALLPEA
                     ETGRGAEALAALPRRGVVLLEEAARARRARREAR"
     gene            4328..4639
                     /locus_tag="VFD06_01845"
     CDS             4328..4639
                     /locus_tag="VFD06_01845"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HZN02307.1"
                     /translation="MTQRGLGVTSILVPAPDIVARDIAGEHLLVPVRSGIAGIDCLYT
                     ADEVGSFVYARLDGRRDAAALARLVSEAYVVEEERALEDVLAFLGELCAAGLARPLES
                     R"
BASE COUNT          620 a         1737 c         1689 g          712 t
ORIGIN      
        1 ggaggtttcc ggcacatcgt actgccgctt gaagaggaag tggtcgtctt cccacaggat
       61 cgtgtcggtg gcggcctggc tgaggaccac ccgcgcggtg atcaggatct cgtagcgccg
      121 ggcgcgcccc tgctcgttga ggatgaccgg cgtcgtcgtg taggtgaaca gcgtcccgat
      181 cagaaccgcg tccgccccct cctcccccgg aaggatccgc acgcggccgc gggaggtgaa
      241 ttcgtccacg acgtgctcgg tcacccgctg ctcgagctcc gggcggctcg tcttgttctc
      301 gaacatcggg atgccgaccg acttgacggt cggcggcagc gtcgagccgt ggccggagag
      361 gtggtagccg caggcggcgg tcaggaggag gagcgcgatg cctgggacga ggcccgcggg
      421 ggagcggcgc gacatcgcgc gcattataac agcgcccctc gggcgcactc gacgcccgcg
      481 cgcgccgcgc gtcactgcac gacgatgttg aggagccgtc ccggcaggta gatcgtcttg
      541 cgcacgctct tcccctcgag gtgcgcgccg actctcgggt cgacccgcgc ccgcgccagc
      601 gcgtccgcct cgggggcgtc ggcggcgacg cgcacgtgcc cgcgcagccg gccgttcacc
      661 tgcacgacca gctccacctc gtcctcgacg agccacgcgg gatccgcctc cggccacggc
      721 tcgagcagga cgctcgtgcc tccccccatc ccctcccaca gcgcctccga cacgtgcggc
      781 gccaggggct gcagcaggag cgccagcgcg cgccccgcct ccttgagata cggctcgtct
      841 cccgcgcgct cctgccgggc gacgtcggtc gtctcgttca ggagttccat gagggaggag
      901 accggcgtgt tgaggtgcag gcggcgatcg aggtcatggg tgacccgctg gatggtgcgg
      961 tgcaccttgc gccgcagcgc ggcgcggcgc ggctcgtccg cgccgtcctt cggaccgggg
     1021 agcggcgccg cagcgaggag ggcggcgcgc ggcgcgaaca gccgccagat ccgctccagg
     1081 aaccggaagc acccctccac cccctggtcg tcccactcga ggtccttctc gggcggcgcc
     1141 gcgaacagcg tgaacaggcg cgtcgtgtcg gcgccgtagc ggcggatgag ctcgtccggg
     1201 tcgacgatgt tccccttcga cttcgacatc gccgtgcctc ccttgagcac catcccctgg
     1261 cacatcatct ggacgaccgg ctcggagaaa cgcacgagcc cgagatcgcg cagcacgcgc
     1321 gtgaagaagc gcgtgtagat gaggtgcaga tgcgcctggt tgatcccgcc gatgtagagg
     1381 tcgatcggga accacgcggc gacgatggcg gggtcgaacg gctgcttcgg gttgcgcgcg
     1441 tcgcagtagc ggtagaagta ccacgacgaa tcgatgaagg tgtccatcgt gtcggtctcg
     1501 cggcgcgcct tgccgccgca gcgcgggcag gtggtcgcga cgaacgccgc cgactgcgcc
     1561 agcggagatc cgccggtgcc ggtcaggggg atgtcctccg gcaggatcac cgggaggtcg
     1621 gcgtcgggga ccggcacgac gccgtcccgc tcgcagtaga tcaccggaat cggcgtcccc
     1681 cagtagcgct ggcgcgaaat cccccagtcg agcaggcggt actgcacgga cgtcctcgcc
     1741 tgtcccttct tttcgccgtc cgcgagcatc cgtgcgcgcg ccgtcgccga gtcgaggccg
     1801 tcgtacggac cggagttgac gagcacgccg tcgtcggtca tggcggcgga cggaaggccg
     1861 tgctcatccg cggaaggcgc ggtggagccg ggacccggcc gcacgaccac gcggatcggc
     1921 agcccgtact gttgcgcgaa ctcgaagtcg cgctcatcgt ggcccggcac cgccatgatg
     1981 gcgccggtgc cgtagcccat caggacgaag ttcccggccc acaccgggat cgcctcgccg
     2041 ctgtacgggt tggtcacccg gagcccggtg tcgactccgc gcttctccgt ctccccggcc
     2101 gtcgcctcgc gccgcggcgc cgccgtggag cgcagctctt cgatcgcgcg cgcctccgtg
     2161 gagccggcgg ggacggcgcg gagaatcgaa tgctcgggcg cgacgacaca gaaggtggcg
     2221 ccgcaaatgg tgtcgaggcg ggtcgtgaag atctcgatcg cgtccgagcc ggctgccccg
     2281 gcgccggcga gagggaagcg gacgatggcc ccctcgctcc ggccgatcca gttctcctgg
     2341 atgctccgca ccgcctccgg ccaggccggc agctccgcaa gccccgccac cagctcctcg
     2401 gagtagcggg agatgcgcag gaaccactgg tcgagctcgc gccggcccac caccgagtcg
     2461 cagcgccagc accggccccc ttccacctgc tcgttcgcca ggaccgtctg acaggaatcg
     2521 caccagttca cttcgcggcg gctgcgatac gcgaggtcct tctccagcat ccgcagaaag
     2581 aaccactggt tccagcggta gtactcgggg cggtgggcgg cgatctcccg cccccagtcg
     2641 tagccgaagc cgagccgccg cagctgcgcc cgcatcgagg cgatgttgtc ggtggtccac
     2701 ttcgccggcg ggatgccgcg ctggatggcc gcgttctcgg ccggcatgcc gaacgagtcc
     2761 cagcccatcg ggtgcaggac ggcttccccc cgcatgcgcc ggaaccgggc caccgcgtcg
     2821 ccgatcgagt agttgcggac gtgccccatg tgcagacgac cggacgggta cggaagcatc
     2881 tccaggcagt agtacttccg cccggtgggg cgctgcggag cgctccaaag gcgctcccgc
     2941 tcccagcgct cctgccagga gcgctcgagg gcgtcgaatt gatcgcggta gtcgccgttc
     3001 atcgcggggc gggattctaa cagaggatgc ggcctcgtcc gtgcgcgccg ccctttcagg
     3061 aacgtaagtg atcgcggcaa gcccgcgccg ggcacggctt ccccggtttc gaggtcgttc
     3121 cggcccttgc gagtctgtca tcgaaaccta tattcgtcac gccgtcgcgg ggggcgaaaa
     3181 cggacatgag atcgagcgct gctttgacac gggacgcggg gtcgttgccc gccgacgacc
     3241 cggtcctcga cccggtcgcg ctcctggcct ctcccggtcc ggcggagcgg gtcgcttcgg
     3301 cgctcctcca ggacctcgac ccggggtcga tcccggtcct cctcgatcgc ctggtctttc
     3361 accgggtcga cggcctcgcc tggcggacgc tgtcctccct ggcgcccggg accgtggacg
     3421 agtggctgcg ggcgacgctc cgacgacggc accaggcgcg ctccgccgcg acgctggcgc
     3481 aggggctggc gctcgccgag atcctcgagg cgtggcaccg cgccggcatc ccggcggcgg
     3541 tgcagcgcgg cctgcgcgtg gtggattgga tctataagga tgcgggggcc cggccgttcg
     3601 aggatcacga tcttctgatc cgcccggccg acgcggaggg ggccgcctcc ctgctgcgtc
     3661 ggctcggcta cgagccgatc ggtcccgcgc tcttccgccg cgccaccgtc ctcgtcgacc
     3721 tgcacaccga cccgctcggt tccgcgcgcc gcccgtcgcg cggccgcatc tttccgttcg
     3781 acgtcgacgc cctgttcgcc gcggcgcgcc ccggctacgt tgccggaggg ccggcgctcc
     3841 tgctgcgcag cgaggacgac ctcgtgctga tggcgctgca cgtcgtcaag cattccttcg
     3901 atcgactgat ccgcagcgcc gacctggcgc acctcctggc cttcgagggg cgggcgctgt
     3961 cgtgggagcg ggtccgcgag acggccgacc gcgcccacgc cacgcggctc gtgaccctcg
     4021 ccctcgccgc gctcacgccg ttcggcatca cgccgccgga accgttccgt ctcgaggagc
     4081 cggtgacggg cctgagcggc ctcctgctgc agcgggtgcg ggcgctccgc ccgctcccct
     4141 acggcggcga gatcctcatg gcgctcgcgg cgccgcgctt cgccgaccgg ctgcgcttcc
     4201 tcttcgacgc gctgctgccg gaggcggaga ccggccgcgg ggcggaggcg ctcgccgccc
     4261 tgccgcgccg aggcgtggtg ctgctcgagg aggcggcgcg cgcgcgacgc gcccgcaggg
     4321 aggcgcgatg acgcagcgcg gcctgggggt gacctcgatt ctcgtgccgg cgccggacat
     4381 cgtggcccgc gacatcgccg gcgagcacct gctcgtcccg gtccgctccg gcatcgccgg
     4441 gatcgactgc ctctacaccg ccgacgaggt cggatcgttc gtgtacgcgc gcctcgacgg
     4501 ccggcgcgac gccgccgcgc tggcgcgcct cgtctccgag gcctacgtgg tcgaggagga
     4561 gcgggcgctc gaggacgtcc tggcgttcct cggagagctg tgtgccgccg ggctcgcccg
     4621 tccgctggag tcgaggtgag ctgcgccgtc gcgtcgtaca gcgccttcgg cacgcgggtg
     4681 atcgaggcgt gcggcggcga gcgcgtgccg ctcaacggct ccatggagct caccttccgc
     4741 tgcaacctgc gctgcgtg
//
LOCUS       DASRIQ010000073         5034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3148803, whole genome shotgun sequence.
ACCESSION   DASRIQ010000073 DASRIQ010000000
VERSION     DASRIQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3148803"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFD06_01850"
     CDS             complement(<1..506)
                     /locus_tag="VFD06_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HZN02308.1"
                     /translation="MRRPRGSFLDAAGPLPIFERPVEAKDSMVQRWLAIGLLLTGLGC
                     MGAWTWEQLDARLFQRSSALRLEALQLKAASIATPGRAPAPKKTRAAGAPIPSGELIG
                     RIEIDRVGLSAIVAEGTDTRTLRRAVGHVPSTPLPGDDGNVVLAGHRDTFFRALKSVE
                     EGDRITVTT"
     gene            complement(515..898)
                     /locus_tag="VFD06_01855"
     CDS             complement(515..898)
                     /locus_tag="VFD06_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HZN02309.1"
                     /translation="MKRSSLLSLGALVLVASVAMTGAVLAQDPPPAGDPDPMMQSATG
                     EPGSQPSNPTYDPNRPSADPYRPADPATPSAADPDRAGDTNDPTRRNLPATASNAPVL
                     LLIGAVALGTLAAFLVLRRRGVTNT"
     gene            1066..3732
                     /locus_tag="VFD06_01860"
     CDS             1066..3732
                     /locus_tag="VFD06_01860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN02310.1"
                     /translation="MRPPVAFDTDVVLRDGTTLHLRALRPEDAAALEDLASRLAPLAA
                     VERGPGWPPQPPAPGAPEEDPARQLTIVGEAGGRLVAIARYTRDERDTERAFVGVAVA
                     EPLQGRGLGTRALEILAASAWEHGLRSFVARVPRGAEARLRLFLDTGYAVEESRDAEA
                     VRLRLSLRPTAASRERAARRSESAAAASLRPFFAPQGIAVVGAGRAPGGIGRAILDNL
                     VAHGYPGPLHAVHPEAARIGAVPAVPRVTEISGPLDLAIVSVPFPAVAAVVEDCLAKG
                     VRALTVITAGYGETGADGREREAALLERVRRAGARMVGPNCMGLLSTDPALPVNATFA
                     PVSPPEGSLAMSTQSGALGLVILDYARSLGLGLSSFVSVGNAADVSTNDLMQYWSADP
                     RTRVILLYVESFGNPRHFSRIARRVGRTKPIVAVKSGRSAAGARAASSHTGALASRDA
                     IVDALFRQSGVIRTDTIEEMFDVGMLLSQQPVPRGRRVAILTNAGGPGILAADACEAH
                     GLALPPLSDATVKALRAFLPPEASVANPVDMIASASAEQYERALSLLLRDEAIDSVLV
                     IFIPPLVTRLEDVAASVRRVAGTRAEKPVLGVFMSARGASALVAPIPCFRFPEAAALA
                     LARVASYGDWLARPESVPPSFPEEERHAAGEVVERALARGGGWMTAAEVQALLAAMRI
                     PAARAEAVASEEAAVAAAEAIGYPVALKGFGPDLLHKTEAGAVILRLTDATALRAAWR
                     DLGTRLGPRMIGGLVQEMVQGGVEMLAGAVEDETFGPVVACASGGTLTEILADAQFRL
                     HPLAESEAREMIDALRGAPLLRGARGAPPADEGALIDVLLRLSALVGEHPEVREIEIN
                     PLVVLPRGARALDARVRVEAPRPAPPDRRIRY"
     gene            complement(3755..4984)
                     /locus_tag="VFD06_01865"
     CDS             complement(3755..4984)
                     /locus_tag="VFD06_01865"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HZN02311.1"
                     /translation="MSDRRHEVLVLGAGAQGNVVATLLARAPDVSRIVLADLDAARAE
                     ETARSIGSDKVRTARADVTAVEATAALMRDGRFDLVVNVAPPQFIPQAMHAALLAERS
                     YLDLSSVALYEIDGLPYEQRQEAEAWERSGRLALINAGSAPGLTNIMARDAADRLDEV
                     ERISIKDFALTESDEYVPLWIPSVYAIDCATEPYVWENGRPERVPIFSGEEMYDFPPP
                     LAVRGKVYLHAHEEPVTIPLHLGRPVQRCEYKIGEPDIDAWRFMVERLRLMDVTPIAV
                     RSGTVSPRDLLLALLPPTPSPQRLAQLVQEGRIASRSMLVVDVEGRRGGKRVRLQTWT
                     EAPDLKEACSIVPGASDISLMTSAPAAVFALMALRGQVRGRGVVLPESLDAEARAVFR
                     DGIAAWGIRIASREIPL"
BASE COUNT          653 a         1823 c         1858 g          700 t
ORIGIN      
        1 gtcgtcacgg tgatccgatc cccttcctcc accgatttga gcgcgcggaa gaaggtgtcc
       61 cggtggccgg ccagcaccac attgccgtcg tcaccaggga ggggcgtgga agggacgtgg
      121 ccgacggcgc ggcggagcgt ccgggtgtcg gtcccctccg cgacgatcgc cgacagtccc
      181 acccggtcga tctcgatccg gccgatcaac tctcccgacg ggatcggcgc ccctgcggcg
      241 cgcgtcttct tcggcgccgg cgcccggccc ggcgtggcaa tcgaagcggc cttcagctga
      301 agcgcctcca gccgcaacgc cgacgaacgc tggaagagcc gggcgtcgag ctgttcccag
      361 gtccaggctc ccatacagcc gaggcccgtg aggagcaggc cgatcgccag ccagcgttgc
      421 accatcgagt ccttcgcctc gaccggccgc tcgaagatcg ggagaggccc cgcggcgtcc
      481 agaaaggaac cgcggggcct cctcacgacc ggtcctaagt gttggtgacg ccacgtcggc
      541 ggagcaccag gaatgcagcc agagtaccaa gcgcaaccgc gccgatcaac agaaggaccg
      601 gagcattgct tgcggtcgcc ggcagattgc ggcgcgtcgg gtcgttggtg tcaccggccc
      661 ggtccgggtc ggcggccgac ggggtcgccg gatcggccgg ccggtacgga tcggcagaag
      721 gccggttcgg gtcgtaggtc gggttcgacg gctgcgaccc cggctcaccg gtggccgact
      781 gcatcatcgg atccggatcg ccggcgggcg gcgggtcctg agccagcaca gcgcctgtca
      841 tggcgacgct ggccacgaga accaaagcgc ccagggacag aagagacgaa cgtttcatat
      901 cggatgtcct ccttgctcac ccgtgaccga gcaaggccga tgccacgctc tcgtttccct
      961 tgaaatcatt gaacggtgcg ttgttcaggg gaatcgactg gtgcgtgaag cgctcccccg
     1021 accgggccgt caggactctc cacgggcggg gtaccatacc ctcgcgtgcg cccgcccgtc
     1081 gctttcgaca ccgacgtcgt cctgagggac ggaacgaccc ttcacctgcg cgccctgcgt
     1141 cccgaagacg cggcggcgct cgaggacctc gcgtcgcgcc tggccccctt ggccgccgtc
     1201 gagcgaggtc ccggctggcc cccccagccg cccgccccgg gagctcccga agaggatccc
     1261 gcccggcagt tgaccatcgt gggggaagcc ggtggacgcc tcgtggcgat cgcccgctac
     1321 acccgcgacg agcgggacac ggagcgggcc ttcgtggggg tggccgtggc cgagccgctc
     1381 caggggcgcg ggctcgggac gcgggccctg gagatcctgg cggccagcgc gtgggagcac
     1441 gggctgcgct ccttcgtcgc ccgcgtgccg cgcggcgcgg aggcccggct gcgcctcttc
     1501 ctcgacacgg ggtacgcggt cgaggagagc cgcgacgcgg aggcggtgcg gcttcggctg
     1561 tccctgcgcc ccaccgcggc gtcgcgcgag cgggcggcca ggcgctcgga gtcggccgcg
     1621 gcggcgtcgc tccgcccctt cttcgcgccg caggggatcg ccgtcgtcgg cgccggccgc
     1681 gcccccggcg gcatcggacg ggccatcctc gacaacctcg tcgcccacgg ctatcccggc
     1741 ccgctgcacg cggtccatcc ggaggccgcg cggatcggcg cggtcccggc cgtgccgcgc
     1801 gtcaccgaga tctccggccc gctcgacctg gcgatcgtca gcgtgccgtt tccggcggtg
     1861 gcggcggtgg tggaggactg cctcgcgaag ggggtgcggg cgctgaccgt gatcacggcc
     1921 gggtacggtg agaccggggc ggatgggcgc gagcgcgagg cggcgctgct cgagcgcgtg
     1981 cgccgcgccg gcgcccgcat ggtcgggccg aactgcatgg ggctcctgag caccgatccg
     2041 gccctgccgg tgaacgccac cttcgctccg gtctctccgc cggagggaag cctggcgatg
     2101 tccacgcaga gcggggcgct cggcctcgtc atcctcgact acgcccgctc gctgggcctg
     2161 ggcctctcga gcttcgtctc ggtcggcaac gcggccgacg tctcgaccaa tgacttgatg
     2221 cagtactggt cggccgatcc gcgcacccgc gtcatcctct tgtatgtcga gagcttcggg
     2281 aacccgcgcc acttcagccg gatcgcccgg cgcgtcgggc ggacgaagcc gatcgtcgcg
     2341 gtgaagtcgg gccggtcggc ggcgggggcg cgggcggcgt cgtcccacac cggcgccctc
     2401 gcctcgcgcg acgccatcgt ggacgcgctc ttccggcagt cgggggtgat ccgcaccgac
     2461 accatcgagg agatgttcga cgtcgggatg ctcctgtcgc agcagccggt cccgcgggga
     2521 cggagggtcg cgatcctgac gaacgcgggc ggtcccggca tcctcgccgc cgacgcctgc
     2581 gaggcgcacg gcctggcgct tcccccgctc tccgacgcga cggtgaaggc gctgcgcgcc
     2641 ttcctccccc cggaggcctc ggtggcgaac ccggtcgaca tgatcgcctc cgccagcgcc
     2701 gagcagtacg agcgggcgct gtcgctcctc ctcagggacg aggcgatcga cagcgtgctg
     2761 gtgatcttca tcccgccgct cgtcacccgc ctcgaggacg tagcggcgtc ggtgcggcgc
     2821 gtcgcgggga cacgggccga gaagccggtg ctcggcgtct tcatgtcggc ccgcggggcc
     2881 tcggcgctcg tggcccccat cccctgcttc cgcttcccgg aggcggcggc gctggccctg
     2941 gcgcgggtcg cgtcgtacgg cgactggctc gcgcgccccg agagcgtccc tccctccttc
     3001 cccgaggagg agcgccacgc ggccggcgag gtcgtcgagc gggcgctcgc gcgcggaggc
     3061 gggtggatga cggcggccga ggtgcaggcg ctgcttgcgg ccatgcgcat cccggcggcc
     3121 cgggccgagg cggtggcgtc ggaggaggcg gcggtcgccg ccgcggaggc gatcggctac
     3181 ccggtcgcgc tcaaggggtt cggtcccgat ctcctccaca agacggaggc gggggcggtg
     3241 atcctgcgtc tcaccgatgc gacggccctg cgcgccgcct ggcgcgatct cgggacgcgg
     3301 ctcggcccgc gcatgatcgg cggactggtg caggagatgg tgcagggggg agtcgagatg
     3361 ctcgccggcg ccgtcgagga cgagacgttc ggcccggtcg tggcctgcgc cagcggcggc
     3421 acgctcaccg agatcctcgc cgatgcgcag ttccgcctgc accccctggc ggagtcggag
     3481 gcacgggaga tgatcgacgc cctgcgcggc gccccgctcc tgcgcggcgc gcgcggcgcg
     3541 ccgccggccg acgagggggc gctcatcgac gtgctgctgc gtctttccgc cctcgtcggc
     3601 gagcatcccg aggtccgtga gatcgagatc aacccactcg tcgtgctgcc gcgcggggcc
     3661 cgggccctcg acgcgcgcgt ccgggtcgag gcgccacgac ccgcacctcc ggaccggcgg
     3721 atccggtact gaaagagata cgccggccga tccgctagag ggggatctcg cgggaagcga
     3781 tccggatccc ccaggcggcg atcccgtccc tgaagaccgc acgggcttcg gcgtcgaggc
     3841 tctcggggag gacgacgccc cgcccccgga cctggccgcg cagggccatc agggcgaaga
     3901 cggccgccgg cgccgaggtc atgagcgaga tgtcgctcgc ccccggcacg atggagcagg
     3961 cctccttcaa gtcgggggcc tcggtccagg tctgaaggcg cacccgcttc cctccccgcc
     4021 gcccttcgac gtcgacgacg agcatcgagc ggctggcgat ccgtccctcc tgcacgagct
     4081 gcgcgaggcg ctgcggcgac ggggtcgggg gaagcagcgc caggaggagg tcgcgaggcg
     4141 acaccgtccc ggagcgcacg gcgatcggcg tcacgtccat caggcgcagc cgctccacca
     4201 tgaagcgcca ggcgtcgatg tccggctccc cgatcttgta ctcgcagcgc tgcaccgggc
     4261 ggccgaggtg cagcgggatc gtcaccggtt cctcgtgcgc gtgcaggtac accttgccgc
     4321 ggacagcgag cggtggaggg aagtcgtaca tctcctcgcc gctgaagatc ggcacgcgct
     4381 cgggccgccc gttctcccag acgtacggct cggtggcgca gtcgatcgcg tagaccgacg
     4441 ggatccagag cgggacgtac tcgtcgcttt ccgtgagtgc gaagtccttg atggagatcc
     4501 gctccacctc gtcgagccgg tcggcggcgt cgcgcgccat gatgttggtg aggccggggg
     4561 cgctgccggc gttgatgagg gcgagacgac cggagcgctc ccaggcctcc gcctcctgcc
     4621 gctgctcgta cggcagcccg tcgatctcgt acagcgccac cgacgacagg tcgaggtagc
     4681 tccgctccgc gaggagcgcg gcgtgcatcg cctgcgggat gaactgcggc ggcgcgacgt
     4741 tcaccacgag gtcgaaccgg ccgtcgcgca tcagggcggc ggtcgcctcg accgcggtga
     4801 cgtcggcgcg cgccgtgcgc accttgtcgc tgccgatgct gcgggcggtc tcctcggcgc
     4861 gcgcggcgtc gagatcggcg aggacgatcc gggagacgtc cggggcgcgc gccagaagcg
     4921 tcgcgacgac gttcccctgc gcgccggccc ccagcaccag cacctcgtgc cgccggtcgc
     4981 tcataggagc gcctcgacgc tctcgcggaa ggcggaggtg tggcgatcga cgtc
//
LOCUS       DASRIQ010000074         8218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1845759, whole genome shotgun sequence.
ACCESSION   DASRIQ010000074 DASRIQ010000000
VERSION     DASRIQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845759"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1061
                     /gene="galE"
                     /locus_tag="VFD06_01870"
     CDS             87..1061
                     /gene="galE"
                     /locus_tag="VFD06_01870"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZN02312.1"
                     /translation="MNLLVTGGAGYVGGAVARRLRAAGHQITILDDLSTGHRHVAAGF
                     DLVECDLADRPAVGAALRGGRIEAIVHFAALALVGASVEAPEEYYDNNVARPLVLLDL
                     ARRAGVKRFVFSSSAAVYGEPERTPIEEDHPTRPTNPYGETKLAFERALRWHAAAHRM
                     AAVSLRYFNAAGATDDGGHGEEHDEETHLIPNVLAAARLGREVAIHGTDYPTPDGTAV
                     RDYVHIEDLAEAHRLALEHPLDPGAAEVFNLGSGRGASVREVIETARRVTGRPIAVRE
                     GPRRPGDPARLVASHDRAARVLGWRPRRGDLASIVASAWGFEQGRTRA"
     gene            1189..2007
                     /locus_tag="VFD06_01875"
     CDS             1189..2007
                     /locus_tag="VFD06_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HZN02313.1"
                     /translation="MQGFIRDGSPEAALLARIDFERLPRHVAIIMDGNGRWARTRNLP
                     RVAGHRAGIAAVRDVVETSARIGCQVLTLYAFSLENWKRPRMEVNTLMSLLREYLRKE
                     LKTLLDHDIAFNVIGRIEELSEPVRRDLEETRAATRDNRGLLFNIALSYGGRAEIVDA
                     CNRLLRERGMAAGPRPGGPPEPVDEETFARYLYTAGQPDPDLLIRTSGEMRISNFLLW
                     QIAYSELWVTETLWPDFRRGELLQAIVDYQKRERRYGRVEATPAPVAAIPGQGR"
     gene            2017..2859
                     /locus_tag="VFD06_01880"
     CDS             2017..2859
                     /locus_tag="VFD06_01880"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZN02314.1"
                     /translation="MPSGIHSGIQRILSAVVFLPIFWVLVKRFGPGPYEALLAVAAGI
                     GLYELYALAAARGHRCHRLLGALTAALVLASFAVPAVPLAAVLAAAVFALPVASLWRG
                     EDWGRVFGDVGATLFAATFVGVTFGYLLSLRLLSDAVSGNETGSDLVFLLFFVVWGSD
                     VFAYAIGRLLGRRPLAPNVSPKKTVEGAIGGLLGAVALAFVARAWFMHRLTVRDALLL
                     GLVLGIVGMLGDLVESMLKRGAGVKDSARLVPGHGGLLDRVDSLLYAAPLLYYYYLFA
                     MRTG"
     gene            2856..4040
                     /locus_tag="VFD06_01885"
     CDS             2856..4040
                     /locus_tag="VFD06_01885"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZN02315.1"
                     /translation="MTKGGGRKRRIAILGSTGSIGVQALDLAAHLEERFEIVALAAGS
                     NADLLARQVLRHRPRLAAVADPSGRAALEAAGRETGCALAFGEEGLLAVAGHPDADVV
                     LAAIVGAAGLRPTWHALKSGRIVALANKESLVMAGALMRETAAAHGAAIVPVDSEHSA
                     VHQCLRGERVEEVERILLTASGGPFRTRPLQSLAAATPEEALRHPVWDMGRKISVDSA
                     TLMNKGLEVIEAHWLFGLPLDRIEVVIHPQGIVHSMVEMADGSVLAQMGTADMRGPIQ
                     YALGAPERLRGPVPRLDLRSSPPLEFLPVEPARYPCLALACAAFRAGGSAPVALNAAN
                     EIAVEAFLGGRLPFPGIARLVEEVLGRHRAAAVPALDDVLECDRSARRAAEEILAMKV
                     LA"
     gene            4037..5344
                     /gene="rseP"
                     /locus_tag="VFD06_01890"
     CDS             4037..5344
                     /gene="rseP"
                     /locus_tag="VFD06_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZN02316.1"
                     /translation="MSWLWEGALPFALVLGSVVLFHEFGHYLAAKMYGITVEVFSVGF
                     GPRLWGFVRRGTEYRLSWVPLGGYVKLKGETSAEDATPPDPGDMMAHSRFRRFVVFVM
                     GAVFNLATAFFLTAFIFMHGVQEYAYLYEAPIIGEIDPESPAVAAGLQPGDKVVSFDG
                     QPVTNWKDLQIRMMLSPRQTRDIVVERDGRPIPARLAIEAGPNEIGRPGLGPATGVLV
                     GGLQPGWPADEAGLKRGDRIAAIDGAPMPTVEKVYRTIQGSPGKALTFTIDRDGSSFD
                     VAITPRDDQGKGKIGFIPTPPTITRTYPFLEAARQSARQNIESIGLVFETFRKLLRRE
                     LSLRAFSGPVDLYRFSGEAMEEGLIPFLQLIAFVSLQLGIINLFPIPPLDGGHLFTLV
                     VEGILRRDLSLVIKERVMQAGLILLLLFMGTVIYFDISKNFFH"
     gene            5355..6452
                     /locus_tag="VFD06_01895"
     CDS             5355..6452
                     /locus_tag="VFD06_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZN02317.1"
                     /translation="MTPPRPDHRILFTPGPTEVVPEILAHMARPVIGHRGEEMQAILG
                     EVIERGRRLFGAAAHELFVTGSSATGLWEAAIRNVVERRVLVPVCGAFSERFFDVAVG
                     CGAEAERLDVEWGRAVDPDQVADRLKSGRFDAVALVHNETSTGVVNPLGPIAEAVRRT
                     PDTALLVDCVSSLAGMPVEVDRHGVDVALASVQKCLALPPGLALCVASARAMERSKRR
                     AGKGYYFDFVRLKGYFDKRNPMATPSVSHIFALQAQLRRIEAEGIEARYARHRAMAEE
                     TRGWAARHDLDLFAAAGARSETVTCVANNRGLEVAAFVKRAAERGYSISNGYGRLKEK
                     TFRIGHMGDHTVEGVRRLLEVLDASLPEGRA"
     gene            6449..8029
                     /gene="serA"
                     /locus_tag="VFD06_01900"
     CDS             6449..8029
                     /gene="serA"
                     /locus_tag="VFD06_01900"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZN02318.1"
                     /translation="MKVLVADKVSATCADVLRAAGFEVEQRPGLALKDLLQAVAGISG
                     LVVRSDTQVTDEVMAAAPGLKVVARAGAGVDNIDVAAATRRGIVVMNAAGENTISAAE
                     HTMSMILALARRIPAADRSMRAGKWERGSAFLGIELFGKTLGILGVGKVGREVAARAR
                     AFGMQVLGYDPVLTAEAAARAGVEAARLDDVVERADVLTLHLPLTDETRHLLNAERIA
                     RCRKGVRIVNVARGGVIDEAALVAALQSGQVAGAALDVFEHEPPTGSPLLGLDSVVLT
                     PHLGASTQEAQEKVAARSAEQVAAYLKDGTVTNAVNAEGVDARTAPALAPYRDLCERL
                     GRLLSALARGPYTELVLESSGAVNEFPLRALTASFLKGLLEGKHSDPVNAVNALVLAR
                     EAGIKVLETRAGESQDFTALVTAILRGPNGSRSVSGTLFGKRDPRLVRVDEFQIDAWP
                     SGPMLLVSNDDRPGMVGRIGTILGEAGVNIAYMSLGRDRSGGRALAVLNLDAPIPDPP
                     LRELATLEGVLWAERVRL"
BASE COUNT         1064 a         2929 c         3017 g         1208 t
ORIGIN      
        1 gcggcgcggg atcgccgggc cgccggcacc tgcgctccgg cgcgtgggct gacgctcgtg
       61 aaggtgttct acgcgggggc cgacgcgtga acctcctggt gacgggcggc gccggctacg
      121 tcggcggggc ggtggcgcgg aggctccgcg ccgcggggca ccagatcacc atcctcgacg
      181 acctgagcac cggacaccgc catgtcgccg ccgggttcga cctcgtggag tgcgatctcg
      241 ccgaccgccc cgcggtcgga gcggcgctcc gcgggggccg gatcgaggcg atcgtgcact
      301 tcgccgccct ggccctcgtc ggggcgtcgg tcgaggcgcc ggaggagtac tacgacaaca
      361 acgtggcccg gccgctcgtg ctcctcgacc tggcgcgccg cgccggggtg aagcgcttcg
      421 tcttctcctc gagcgccgcg gtgtacgggg agccggagcg cacgccgatc gaggaggacc
      481 atcccacccg cccgacgaac ccgtacggcg agacgaagct ggcgttcgag cgcgccctgc
      541 gctggcacgc ggcggcgcac cggatggcgg cggtgtcgct gcgctacttc aacgccgccg
      601 gcgcgaccga cgacggcggc cacggcgagg agcacgacga ggagacccac ctgatcccga
      661 acgtgctcgc ggcggcccgg ttgggaaggg aggtggcgat ccacggcacc gactacccga
      721 cgccggatgg caccgcggtg cgcgactacg tgcacatcga ggacctcgcc gaggcgcacc
      781 gcctggcgct cgagcacccg ctcgatccgg gcgcggcgga ggtcttcaat ctcggcagcg
      841 gccgcggcgc ctcggtgcgc gaggtcatcg agaccgcgcg ccgggtgaca ggccggccga
      901 tcgcggtgcg ggaggggccg cggcggccgg gcgacccggc gcgactcgtc gcgtcccacg
      961 atcgcgccgc gcgcgtgctc ggctggcgcc cccggcgcgg cgatctcgcg tcgatcgtcg
     1021 cctccgcgtg gggtttcgag cagggccgca cgcgcgcctg aggaggcacg cgccccggcg
     1081 ggccggccgc gcggcgcatc cttggcgccc gcggaaggcg tgtgctacga tgcgcgccga
     1141 acgcgcgagg aaagagcgca gccccgcatg cccgatccat tgaccccaat gcagggattc
     1201 atccgagacg gcagccccga ggccgcgctc ctcgcccgga tcgacttcga gcggctgccg
     1261 cggcacgtcg ccatcatcat ggacggcaac ggccgctggg cccggacgcg caacctcccg
     1321 cgcgtcgccg ggcaccgcgc cgggatcgcc gcggtccgcg acgtggtcga gacctcggcc
     1381 cgcatcggct gccaggtcct gacgctgtac gccttctcgc tcgagaactg gaagcggccg
     1441 cgcatggagg tgaacaccct gatgtcgctc ttgcgcgagt acctgcgcaa ggagctgaag
     1501 acgcttctgg atcacgacat cgccttcaac gtcatcgggc ggatcgagga gctctccgag
     1561 ccggtgcggc gcgacctcga ggagacccgc gccgcgacgc gggacaaccg cggcctgctg
     1621 ttcaacatcg cgctgtcgta cggcggccgg gcggagatcg tcgacgcctg caaccgtctg
     1681 ctgcgcgagc gcgggatggc ggccggtccg cgccccggcg gcccccccga gccggtggac
     1741 gaggagacct tcgcacgcta cctgtacacc gccggccagc ccgatcccga cctgctcatc
     1801 cgcacctccg gcgagatgcg catcagcaat ttcctgctgt ggcagatcgc ctactccgag
     1861 ctctgggtca ccgagacgct ctggcccgac ttccggcgcg gcgagctcct gcaggcgatc
     1921 gtcgactacc agaagcgcga gcggcgctac gggcgggtcg aggccactcc ggctccggtc
     1981 gccgccatcc ccggtcaggg ccggtagaac tccgccttgc cctcgggcat tcactcggga
     2041 attcagcgga tcctctcggc cgtcgtcttc ctgccgatct tctgggtcct ggtgaaacgc
     2101 ttcggtcccg gcccctacga ggcgctcctc gccgtcgccg ccgggatcgg cctctacgag
     2161 ctgtacgcgc tcgccgccgc ccggggtcat cgctgccacc gcctgctcgg ggcgctcacc
     2221 gccgccctgg tgctcgcctc gttcgccgtg ccggcggtgc cgctcgcggc ggtgctggcc
     2281 gcggccgtct tcgcgcttcc ggtggcgtcg ctgtggcgcg gcgaggactg ggggcgcgtc
     2341 ttcggcgacg tcggggcgac cctcttcgcc gcgacgttcg tcggcgtgac gtttggatac
     2401 ctcctgtcgc tgcggctcct cagcgacgcg gtctccggga acgagaccgg gagcgacctg
     2461 gtcttcctcc tcttcttcgt ggtctggggg agcgacgtgt tcgcgtacgc gatcggccgc
     2521 ctcctcgggc gccgtccgct ggccccgaac gtcagcccca agaagaccgt cgaaggggcg
     2581 atcggcgggc tcctgggggc ggtggcgctg gcattcgtgg cgcgcgcctg gttcatgcac
     2641 cgcctgacgg tgcgggacgc gctcctgctc ggcctcgtgc tcgggatcgt cgggatgctc
     2701 ggcgacctgg tggagtcgat gctcaagcgc ggcgccgggg tgaaggacag cgcccggctc
     2761 gtccccgggc acggcgggct cctcgaccgc gtcgattcgc ttctctacgc ggcaccgctt
     2821 ctctactact attacctgtt cgcgatgagg accggatgac gaaggggggc ggccgcaagc
     2881 gccgcattgc gattctcggc tcgaccgggt cgatcggggt gcaggccctc gatctcgcgg
     2941 cgcacctcga ggagcgcttc gagatcgtcg ccctggcggc cggatcgaac gccgatctcc
     3001 tggcgcgcca ggtgctgcgg caccgtccgc gcctggccgc cgtcgccgac ccgtccgggc
     3061 gcgcggcgct cgaagcggcc gggagagaga ccggctgcgc gctcgccttc ggggaggagg
     3121 ggctgctggc cgtcgccggg catcccgacg ccgacgtggt gctggcggcg atcgtcggcg
     3181 cggcggggct ccgcccgacc tggcacgcgc tcaagagcgg ccgcatcgtc gcgctcgcca
     3241 acaaggagag cctggtgatg gcgggggcgc tgatgcgcga gaccgccgcg gcccatggcg
     3301 ccgcgatcgt cccggtggac agcgagcact cggcggtgca ccagtgcctg cgcggcgagc
     3361 gggtggagga ggtggagcgg atcctcctca ccgcctccgg tgggccgttc cggacacgcc
     3421 cgctccagag cctcgccgcg gcgaccccgg aggaggcgct gcgccatccg gtgtgggaca
     3481 tggggcgcaa gatcagcgtc gacagcgcca cgctcatgaa caaggggctg gaggtgatcg
     3541 aggcgcactg gctcttcggc ctgcctctgg accggatcga ggtcgtgatc catcctcagg
     3601 gcatcgtgca ctcgatggtg gagatggcgg acgggtcggt gctggcccag atggggacgg
     3661 ccgacatgcg cggccccatc cagtacgccc tcggcgcgcc ggagcggctc cgcgggcccg
     3721 tgccgcggct cgatctgagg tcgtcgccgc cgctcgagtt cctgccggtg gagcctgcgc
     3781 gctacccgtg cctcgctctg gcgtgcgccg ccttccgcgc cggggggagc gcgccggtgg
     3841 cgctcaacgc ggcgaacgag atcgccgtcg aggcgttcct cggcgggcgg ctcccgttcc
     3901 ccggcattgc ccgcctggtg gaggaggtgc tcgggcgcca ccgggccgcg gccgtcccgg
     3961 ccctcgacga cgtcctggag tgtgatcggt ccgcgcgccg cgcggcggag gagatccttg
     4021 cgatgaaggt gctggcgtga gctggttgtg ggagggggcg ctgccgttcg cgctggtcct
     4081 cgggtcggtg gtgctgttcc acgaattcgg tcactacctc gccgccaaga tgtacggcat
     4141 cacggtcgag gtcttctcgg tcgggttcgg gccgcgtctg tggggcttcg tgcggagggg
     4201 gaccgagtac cgcctctcgt gggtcccgct cggcggatac gtcaagctca agggggaaac
     4261 ctccgccgag gacgcgacgc cgcccgatcc gggcgacatg atggcccact cgcgcttccg
     4321 ccgcttcgtg gtgttcgtca tgggcgcggt gttcaacctc gcgaccgcgt tcttcctgac
     4381 cgccttcatc ttcatgcacg gcgtgcagga gtacgcctac ctgtacgagg cgccgatcat
     4441 cggcgagatc gacccggagt cgccggccgt cgccgccggg ctgcagccgg gcgacaaggt
     4501 cgtctccttc gacggccagc cggtgacgaa ctggaaggat ctgcagatcc ggatgatgct
     4561 cagcccgcgg cagacgcgcg acatcgtcgt cgagcgggac ggacggccga ttccggcgcg
     4621 gctcgccatc gaggcggggc cgaacgagat cggccgtccg ggcctggggc cggccaccgg
     4681 cgtgctggtc ggggggctgc agccggggtg gccggcggac gaggcggggc tgaagcgcgg
     4741 cgaccgcatc gccgccatcg acggcgcgcc gatgccgacc gtcgagaagg tctaccgcac
     4801 gatccaggga tcgccgggga aggccctgac cttcacgatc gatcgggacg ggagctcctt
     4861 cgacgtggcg atcacgccgc gcgacgatca gggaaagggg aagatcggct tcatccccac
     4921 gccgccgacg atcacccgca cctatccgtt cctcgaggcg gcccgccaga gcgcccgcca
     4981 gaacatcgag tcgatcggcc tggtgttcga gaccttccgc aagctgctgc ggcgcgagct
     5041 gagcctgcgc gccttctccg gtccggtcga tctctaccgc ttctccgggg aggcgatgga
     5101 ggaggggttg atcccgttcc tccagctgat cgccttcgtc agcctgcagc tggggatcat
     5161 caacctgttc ccgatcccgc cactcgacgg cgggcacctg ttcaccctcg tcgtcgaggg
     5221 gatcctgcgg cgcgacctgt cgctggtgat caaggagcgg gtgatgcagg cggggctcat
     5281 cctgctgctg ctgttcatgg gaacggtcat ctacttcgac atcagcaaga acttcttcca
     5341 ctgaggcccg attcatgacg ccgccgcgcc ccgatcaccg catcctgttc acccccgggc
     5401 cgaccgaggt cgttcccgaa atcctcgccc acatggcgcg accggtcatc ggtcaccgcg
     5461 gcgaggagat gcaggcgatc ctcggggagg tgatcgagcg cggccggcgg ctgttcggcg
     5521 cggcggcgca cgagttgttc gtcaccggct cctccgcgac cgggctgtgg gaggcggcca
     5581 tccgcaacgt cgtcgagcgc cgcgtgctgg tgccggtgtg cggcgccttc tcggagcgct
     5641 tcttcgacgt ggcggtcggc tgcggcgccg aggcggagcg gctcgacgtc gagtgggggc
     5701 gggcggtcga tcccgatcag gtcgccgatc ggctgaagtc cgggcgtttc gacgccgtcg
     5761 ccctcgtgca caacgagact tcgaccgggg tcgtcaaccc gctcggcccc atcgccgagg
     5821 cggtgcgccg cacgccggac accgcgctcc tcgtcgactg cgtctcgtcg ctcgccggca
     5881 tgccggtgga ggtcgaccgc cacggcgtcg acgtggccct cgcctcggtg cagaagtgcc
     5941 tggcgctgcc gccgggcctg gcgctgtgcg tcgcctcggc gcgcgccatg gagcgctcga
     6001 agcgccgcgc gggcaagggg tactacttcg acttcgtgcg cctcaagggg tatttcgaca
     6061 agcgcaaccc gatggcgacg ccgtcggtca gccacatctt cgccctccag gcgcagctgc
     6121 ggcgcatcga ggcggagggg atcgaggcgc gctacgcccg ccaccgggcc atggccgagg
     6181 agacgcgcgg ctgggccgcg cgccacgacc tcgacctgtt cgcggcggcc ggggcccgct
     6241 ccgagaccgt cacctgcgtc gccaacaacc ggggcctcga ggtcgccgcc ttcgtgaagc
     6301 gggcggcgga gcgcggctac tcgatcagca acggctacgg gcggctcaag gagaagacct
     6361 tccgcatcgg ccacatgggg gatcacaccg tcgagggggt gcggcggctc ctcgaagtcc
     6421 tcgacgcgtc gctcccggag ggccgggcgt gaaggtcctc gtcgccgaca aggtttctgc
     6481 cacctgcgcc gacgtgctgc gcgccgccgg cttcgaggtg gagcagcgcc ccgggctcgc
     6541 cctgaaggat ctcctgcagg cggtcgccgg gatcagcggg ctcgtggtcc gatccgacac
     6601 ccaggtcacc gacgaggtga tggcggcggc gccgggcctc aaggtggtcg cccgcgccgg
     6661 cgccggcgtg gacaacatcg acgtcgcggc cgcgacccgc cgcggcatcg tcgtcatgaa
     6721 cgcggcgggg gagaacacca tctccgccgc cgagcacacg atgtcgatga tcctggcgct
     6781 ggcgcgtcgg atcccggcgg ccgatcggtc gatgcgcgcc ggaaagtggg agcgcggctc
     6841 cgccttcctg gggatcgagc tgttcggcaa gacgctcggg atcctcggcg tcggcaaggt
     6901 ggggcgcgag gtggcggccc gggcgcgtgc cttcggcatg caggtgctgg gctacgaccc
     6961 ggtccttacc gccgaggcgg cggcgcgcgc cggcgtcgaa gccgcccggc tggacgacgt
     7021 ggtggaacgg gcggacgtgc tcaccctgca cctgccgctg accgacgaga cgcggcacct
     7081 cctgaatgcc gagcggatcg cccgctgccg caagggggtg cggatcgtca acgtggcgcg
     7141 cggtggcgtc atcgacgagg cggcgctcgt cgcggcgctc cagagcggcc aggtcgccgg
     7201 ggcggcgctc gacgtcttcg agcacgagcc gccgaccgga tccccgctcc tcggcctcga
     7261 cagcgtcgtg ctgacgccgc acctgggggc ctcgacccag gaggcgcagg agaaggtcgc
     7321 cgcccggagc gccgagcagg tggcggcgta cctcaaggac ggcaccgtca ccaacgcggt
     7381 caacgccgaa ggggtggacg cgcgcacggc gccggccctg gcgccttacc gcgatctgtg
     7441 cgagcggctc gggcggctcc tctccgccct cgcccgcggc ccgtacaccg agctggtcct
     7501 cgagtcgagc ggggcggtga acgagttccc gctccgcgcc ctgaccgcct cgttcctgaa
     7561 ggggctcctg gagggaaagc actccgatcc ggtgaacgcg gtcaacgcgc tggtcctggc
     7621 gcgcgaggcg gggatcaagg tgctggagac gcgggccggc gagtcgcagg acttcaccgc
     7681 gctcgtgacc gccatcctgc gcgggcccaa cggctcccgg tcggtctcgg gaacgctgtt
     7741 cggcaagcgc gatccgcgcc tggtgcgggt cgacgagttc cagatcgacg cctggccctc
     7801 cggcccgatg ctcctggtga gcaacgacga tcgccccggc atggtcggcc gcatcggcac
     7861 catcctcggc gaggcgggcg tcaatatcgc ctacatgtcg ctgggccgcg atcgctccgg
     7921 agggcgcgcc ctcgcggtgc tcaatctcga cgcgccgatt cccgatcctc ctctccgaga
     7981 actcgcgacc ctggaggggg tcctctgggc cgagcgggtg cgcctctagc ccgaagcggc
     8041 gccggcccgc gtccccgcga ggccgcgatg gttgaccagc atgaatgagg tgggggagaa
     8101 ttccgccgag ggaacccccc catgctgcgg ccgagaggcg tccgtgaggc gacgctcgca
     8161 gtgctgttcg gggccgcgat cgtcctgctg caacgaagcc ctcttgccgc cgcggacg
//
LOCUS       DASRIQ010000075         8493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_549863, whole genome shotgun sequence.
ACCESSION   DASRIQ010000075 DASRIQ010000000
VERSION     DASRIQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549863"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFD06_01905"
     CDS             <1..697
                     /locus_tag="VFD06_01905"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZN02319.1"
                     /translation="LELAAAGEPEGGLVVAEEQTEGRGRRDRSWASSPSLGIYASLIL
                     RPAIPAPRAPLLTFMAAIATADALNEIPGLKARIKWPNDVTVSGRKIAGVLGEVRGSD
                     PEVREMVVGIGVNVHHLESDFPPELVARATSVRLATGRGWDRAALLARLLEDFEQGYT
                     RALRDGPGTLLRDWQSLSALQPGDPVRVLGPAGPAQGAFSGIDEEGGLRLVVDGGRTL
                     RVPFGEIVETLEP"
     gene            711..1478
                     /locus_tag="VFD06_01910"
     CDS             711..1478
                     /locus_tag="VFD06_01910"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZN02320.1"
                     /translation="MLLVMDVGNTNTVLGVYDGTTLVAHWRLTTVRDRTVDEYGILAR
                     NLLSLARIDAKAIDGVIIASVVPPLDGVLEAMARTYFGLTPLFVEPGVKTGMPILYDN
                     PQEVGADRIVNGVAAYERCRQAVIVVDFGTATTFDAISAKGEYLGGAIAPGLAISAEA
                     LFQRAARLPRVDVRKPPKVIGRNTTHSLQSGLFFGYLALVTGILSRMRAEMGEPVRTI
                     GTGGLSAALKSELSGLLDEVDPNLTLEGLRLLHDRNR"
     gene            1475..2188
                     /locus_tag="VFD06_01915"
     CDS             1475..2188
                     /locus_tag="VFD06_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02321.1"
                     /translation="MIEGEQEDPYLLAIEETFNRRRGAPHLLSPRDWALADSWRRSGV
                     PLRLVLQGIENCFDAFERRAPGPRRINSLSYCRQEVLGLHDLYVRLHGVEAGRPDATA
                     TPAEATSALAKHLGRLVRRLREAATAASRDGRDALVGALAEAAAEVRLLRKSLAGAPA
                     DPAAVEASLDRLDAALLDRARAVLTDEESHVIEAAVDAALAEGGTRLTGAALATTRAA
                     ARARRIRQTAKLPRLSLFD"
     gene            2391..4664
                     /locus_tag="VFD06_01920"
                     /pseudo
     CDS             2391..4664
                     /locus_tag="VFD06_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
     gene            complement(5684..5953)
                     /locus_tag="VFD06_01925"
     CDS             complement(5684..5953)
                     /locus_tag="VFD06_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZN02322.1"
                     /translation="MIKSDIINSVARTAEITRVRAVVAVEAILDAMKESMRRGERIEL
                     RGFGVFQVKPRKKGIGRNPRTGTEVRIPPGKTIRFKPGKNLRNLR"
     gene            6084..7397
                     /gene="der"
                     /locus_tag="VFD06_01930"
     CDS             6084..7397
                     /gene="der"
                     /locus_tag="VFD06_01930"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZN02323.1"
                     /translation="MPRPIVAIVGAPNVGKSTLFNRLLGRRQAIVADEPGVTRDRLTA
                     EAEIGARNVTVVDTGGVTSGDRDDLARRVREEALKAAEMADVILFIIDGRAGLTAADE
                     HVATLLRASGRPVIPVVNKIDSRSQEGFEFEMFRLGFDEVLPISAEEGRGLVELVEKI
                     EALLPPEQHAAEDPGVPVAIVGRPNVGKSSLFNCLLRDERSVVSELPGTTRDPVDATF
                     THAGTLYRVVDTAGIRRRVGGAGALEWVSALKARQALERASVAIGLVDVTLGIEHQDR
                     ALLGLILETRKPAVLGVNKVDLLPGGEAAVHERLETLREGLRFSPHVPLVPLSAKTGR
                     GVGTLLKTLENLREESARRFTTPVLNRALESILAERHPPTDNGRAVQLHYITQAPGSP
                     PRFVVFGNGRRITPAYRRFVEHRLRDRLGLTHAPITLLFRRKPSR"
     gene            7444..8010
                     /locus_tag="VFD06_01935"
                     /pseudo
     CDS             7444..8010
                     /locus_tag="VFD06_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
BASE COUNT         1168 a         3118 c         3025 g         1182 t
ORIGIN      
        1 gctcgagctc gcggccgccg gcgagccgga aggcggcctg gtggtggccg aggagcagac
       61 ggagggacgc ggccggcgcg atcgctcgtg ggcgtcgagc ccctctctcg ggatctacgc
      121 ctcgctcatc ctgcgccccg ccatcccggc gccgcgggcg ccgctcctga ccttcatggc
      181 ggcgatcgcg accgccgacg cgctcaacga gatccccggg ctcaaggcgc gcatcaagtg
      241 gccgaacgac gtcacggtgt cgggccggaa gatcgccggc gtcctcgggg aggtgcgcgg
      301 ctccgacccg gaagtgcgcg agatggtcgt gggcatcggc gtcaacgtgc accacctcga
      361 gtccgacttc ccccccgagc tcgtggcgcg cgccacctcg gtgcgcctgg cgaccgggag
      421 gggatgggac cgcgcggcgc tcctggcgcg gctgctcgaa gacttcgagc agggttacac
      481 gcgggcgctg cgcgacgggc cggggacgct cctgcgcgac tggcagagtc tctcggcgct
      541 gcagccgggg gacccggtcc gggtgctcgg cccggccggc ccggcgcagg gagcgttcag
      601 cggcatcgac gaggaggggg ggctgcggct ggtggtcgac ggcggccgta ccttgcgcgt
      661 gccgttcggc gagatcgtcg agacgctcga gccatgactt cgaggacgcc atgctgctgg
      721 tgatggacgt cggcaacacc aacaccgtcc tcggcgtcta cgacgggacg acgctcgtgg
      781 cgcactggcg cctcacgacc gtccgcgacc gcaccgtgga cgagtacggc atcctggcgc
      841 gcaacctcct gtcgctggcg cgcatcgacg ccaaggcgat cgacggggtc atcatcgcgt
      901 cggtcgtgcc gccgctcgac ggcgtcctcg aggcgatggc gcgcacctac ttcggcctga
      961 ctccgctgtt cgtcgagccc ggcgtgaaga ccggcatgcc gatcctctac gacaatcctc
     1021 aggaagtggg tgcggatcgg atcgtcaacg gggtcgcggc gtacgagcgc tgccggcagg
     1081 cggtcatcgt cgtcgacttc ggcaccgcca ccaccttcga cgccatttcg gcgaaggggg
     1141 agtacctcgg cggcgccatc gcccccgggc tcgccatctc cgccgaggcg ctcttccagc
     1201 gggcagcgcg cctgccgcgc gtggacgtgc ggaaaccccc caaggtcatc ggccgcaaca
     1261 ccacccacag cctgcagtcg gggctcttct tcggctacct ggcgctcgtc accggcatcc
     1321 tctcccggat gcgcgccgag atgggggagc cggtccgcac catcggcacc ggcgggctgt
     1381 cggcggcgct caagagcgag ctgtccggac tcctcgacga ggtcgatccg aacctcaccc
     1441 ttgaagggtt gcgtctcctc cacgatcgca accgatgatc gagggggagc aggaggaccc
     1501 ctacctcctg gcgatcgagg aaaccttcaa ccggcgccgg ggcgcgccgc accttctcag
     1561 cccgcgcgac tgggccctgg ccgactcgtg gcgccgctcc ggcgtgccgc tgcgcctggt
     1621 gctccagggg atcgagaact gcttcgacgc cttcgagcgc cgcgcccctg gtccccggcg
     1681 catcaactcg ctgtcgtact gccgccagga ggtgctcggc ctgcacgacc tctacgtgcg
     1741 gctgcacggc gtcgaggcgg ggcggccgga cgcgaccgcg accccggcgg aggcgacgtc
     1801 ggcgctcgcg aagcacctcg gccgcctggt ccgcaggctg cgcgaggcgg cgaccgcggc
     1861 ctcgcgcgac ggacgcgacg cgctggtcgg cgcgctggcg gaggcagccg ccgaggtgcg
     1921 tctcctgcgc aagagcctgg ccggagcccc cgccgacccg gccgccgtcg aggcgagcct
     1981 cgatcgactg gacgcggccc tgctcgatcg ggcccgcgct gtcctgaccg acgaggagag
     2041 ccacgtcatc gaggccgccg tcgatgccgc gctcgccgag ggggggacgc gcctgaccgg
     2101 agcggcgctc gcgacgaccc gcgcggcggc gcgcgcccgg cggatccgac agaccgcgaa
     2161 gctgccccgc ctcagcctct tcgactgact tgatccgggg gccggccctg gtttagggtc
     2221 ggcgcacccg ctggagagcc gggagatgcg agcccggcgt gtccccgtca cgcgtcccga
     2281 cgttcccgcc ttcggcccgt cgtgcgtcga gcctcgccct ggcgttcgcg gccggcgtcg
     2341 cagtcgcgtc ccccggagac ggggaggcgc gcctcgtccc tttcgccgcg atgctcgtcc
     2401 cggccctcct cctcggatgc gacgcgcgcc cgcggcggag cctcgccgcg ggcgcggcgc
     2461 ggctcgtcct cctcctctcc gcggggatcg ccgcggccgg gatggcgcgg cgcgcggaca
     2521 ccctcccggc gctcctggcc tcctggaccg tgcgcggctt cgcggagcac cggacccctg
     2581 tccgcatccg cggcgtcatc gccgatctgg agcgccccga cccggaccgc gcggtcctca
     2641 tcgtccgctc gctcggagcg ccggaaggtt ccgcccggtt cgtcctgccc cggggagcgg
     2701 gggtgcgtct tgcggtgccg atcgacccgg aggggccgct cccgtggcgc atcggcgacc
     2761 gcctcgagac gaccgcccgc atcggccggc cgcgagcctg gcgcaacccc ggcgcgttcg
     2821 attacgcggc gtccctcaag agccgcgaca tcgacctgac cggatcgatc aagagcccgc
     2881 gtctcgtgga gccggcgggc cgcgagccgt tccggcggca cctcggagcg cgggtgcgtc
     2941 aaaggctcgc gcggggtctc gagacggccg ccgacgaagc ggaccggccg accgcgtcgt
     3001 tcctgctggc gctcctcctc ggcgaacggc aagccctcgc gcccgatctc gaggatcgcc
     3061 tgaagcgagc cggcgtctac cacatcctcg ccctctcggg attcaacgtc gcgctggtgg
     3121 cgggaggggc ggcgctcgtc ctccgcctcg tcgtccgtgc accgaccgtc cgtcgccttc
     3181 ttctcgccgg cgtcgtgatc ctctatgcgg cgatcgcccg gcccggcgga tcgatttcgc
     3241 gcgcggcgct gatggtcctg ctgctgctcg cggcgcggca ggccggcagg cgcatgaggc
     3301 ccctcggagc gctcgcggcc tcgtcgacga tcctcctcgc ggcgaagccg gcctggctgt
     3361 tcgatcccgg cttccagctg agctatgccg cgacggcggg gctcctgctc gccggtcgtg
     3421 gatggggagg ggacggggac gcggagcgat ccacccatac gccgaccctg cggcccgtgc
     3481 gtgcgctgtg gtggctgcgc gcccaaacct tggggttact cgcggcctcc gccgcggcgc
     3541 ttgtcgcgac cgccccgctg accgcccggc acttccacac cgtcaccctc gcgggtgtcg
     3601 tcgcgaacct ggcggcggtg ccgctctcgg ccgcgtgcct cggcatcggc gcgatcgccg
     3661 cgccactggc gctcctgtgg cccggcgccg cctccgcgct cgtcgccgca gcgacgccgc
     3721 tcgtcggtct cttgacctgg agcgccggcc tggccgccga tctcccgggc gcctctttct
     3781 tcatccagcc tccctcgcgg cggatggtcg cgctgctctt cctgctcacg gcggcgggag
     3841 cgctcctgcc gcgcggccgc cggcgccggt gcgcgtggct gctcctggtc gcgctcgcgg
     3901 cggccctggc gtcgcggggc cggttgccgc ggtccaccgg acgcctggat gtcgtggtct
     3961 tcgatgtcgg gcagggggac gcgatcctgg tgcggctccc ttcgggcggg gcgatgctca
     4021 tcgacgcggg cgggctgccg cgcggagatt tcgatccggg ggcacgtgtc gtggcgccgg
     4081 ctctgcgcgc cctcggagtg ctgcggttgg atctcctcgt cctcacccac ccgcaccgcg
     4141 accacatcgc aggagggccg gcgatcctcg ggatgttccg ccccgaagcg gtctgggtcg
     4201 gcgccgaggg ggaggacggc cgggtcgctc cggtcgagcg ggcggcggag gcggtcggcg
     4261 cttccgtgct gcggccgcgc ggcgggatcg cactgcggtt cggtggtgcg aggctggagg
     4321 tgctgaaccc gcccggcccg gcatcgcgtg gcccgcgcac gggaaaggca gggaacgacg
     4381 attcgatcgt gatccgcctc cgttacggac ggcgctccgt ccttctgacg ggggacatgg
     4441 aacggccggc ggaggagcgt ctcctcgcgg agcggcggcc gctccggacc gatctcctca
     4501 aggtggcaca ccacggcagc gacacctcca cgtccgaatc gttcctcgag gcggtcgccc
     4561 ccctggcggc ggtggtctcc gccggggcgt cgaacccgtg gggacatccg tccccccaag
     4621 tcctcggccg gctcgaccgg gccggcgtcg cgatcttccg gaccgatcgg cacggcgcgg
     4681 tgcgcgcccg caccgacgga ctgtccccct ggcgcatcga acccctgacg tcagcgccag
     4741 gcgatcccga acacctgggg agcgatcggg aagaaggtca ggacgaacag gagcaggccg
     4801 aacacggcga cggcgatgcg gccgccgccg agcggctcga tctcgtcgag gggccgcggg
     4861 tgccggatgc cgaggatccc gaagaggact ccgaacacca ccagatggta tccgccgaag
     4921 agaagaccgt agaccaccag gaccgcgatc ccggcgcgcg agacgaacgc gtgcacgcgc
     4981 gccgacagcg cgtacagcac gtggccaccg tccagctgcc cgatcggcag caggttcagc
     5041 gaggtggcga acagcccgag ccaggcggcg acgaagagcg ggtggaacga gatggtcatc
     5101 ccctcgggcg gctggaagaa gagcgggtac aggagcgaca ggaggaggca cggcgacagt
     5161 cccacgtccc ccggagacgg ggcgatcggg acgacggtcg aacgggccag accgtagacg
     5221 aggatcggca ggagcgccac gaatccggcg atcggcccgg cgatcccgac gtcgaacagg
     5281 gcgcgccggg tggggaaggg ggcgcggatc cggatcaccg cgccgaacgt gccgaacagg
     5341 ttcggtccgg gaaggaagta cgggagcgag gaatcgatcc ggtacagccg gcaggcgacg
     5401 taatgcccca tctcgtggca gccgaggatg aacagggcgg cgagggaata cggcagaccg
     5461 gccccccagg tgcgcggcgt ccggaggatc ctgaggagga gctcgaacga gaacggctcc
     5521 ggccccgaga gcaccacccc cacgacggtc gtcgacagga gcgtcagcag gaagaggagc
     5581 aggtggatcg ggcggcgcgc cggccgcggc gacggctcct ccggcgggcg cggcacggcc
     5641 cagggttcgg gcggggccgg aggaaggcct ggatccaact caactacctg aggtttcgga
     5701 gattcttccc gggcttgaaa cggatcgtct tgccgggcgg gatacggacc tcggtcccgg
     5761 tcctcgggtt gcggccgatc cctttcttgc gcggcttcac ctggaacacc ccgaagccgc
     5821 ggagctcgat tcgttccccc cgccgcatcg actccttcat cgcgtccagg atcgcctcga
     5881 ccgccaccac cgctctgacc cgggtgatct cggccgtgcg ggccacacta ttaatgatgt
     5941 cgctcttaat cacgcctttt ccccccgacc tgccgaggct ggcagcggag tataggaggt
     6001 aagtaccgga ctgtcaagcc tttgcggcgg tccccatgct aagattccac cccttcgccc
     6061 ggctctccgg gcggtgaatc ccgatgccac gaccgatcgt cgccatcgtc ggcgccccca
     6121 acgtcggcaa atcgaccctc ttcaaccgcc tgctcgggcg gcggcaggcg atcgtcgccg
     6181 acgagccggg ggtgacccgg gaccggctca ccgccgaggc agagatcggg gcccggaacg
     6241 tcaccgtggt cgacaccggc ggcgtcacgt ccggcgaccg cgatgacctg gcccgccgcg
     6301 tccgcgagga ggcgctcaag gcggctgaga tggcggacgt catcctgttc atcatcgacg
     6361 gccgggccgg cctgacggcg gccgacgagc acgtcgcgac ccttctgcgc gcctccggcc
     6421 ggccggtgat cccggtggtc aacaagatcg acagccggtc gcaggagggg ttcgagttcg
     6481 agatgttccg gctggggttc gacgaggtgc tgccgatctc cgcggaggag gggcgcggcc
     6541 tcgtcgagct ggtcgagaag atcgaggcgc tcctgccgcc cgagcagcac gcggccgaag
     6601 accccggtgt gccggtggcg atcgtcggcc gcccgaacgt cggcaagtcg tctctcttca
     6661 actgcctgtt gcgggacgag cgctcggtcg tgtcggagct gccggggacg acgcgcgatc
     6721 cggtggacgc gaccttcacg cacgcaggca cgctgtaccg cgtcgtcgac accgccggga
     6781 tccggcggcg ggtcggcggg gccggcgccc tcgaatgggt gagcgcgctc aaggcacggc
     6841 aggccctgga gcgcgcctcg gttgccatcg gcctggtcga cgtgacgctc ggcatcgagc
     6901 accaggatcg ggcgctcctc ggcctcatcc tcgagacgcg caagccggcg gtgctcggcg
     6961 tgaacaaggt cgatctcctg ccgggcgggg aggcggccgt gcacgagcgg ctcgagacgc
     7021 tgcgcgaggg gctgcgcttc tcgccgcacg tcccgctcgt gccgctgtcg gcgaagaccg
     7081 ggcgcggggt gggcaccctc ctgaagacgc tggagaacct gcgcgaggag tccgcccgcc
     7141 gcttcacgac cccggtcctc aaccgcgcgc tcgagtcgat cctggcggag cggcaccctc
     7201 ccacggacaa tggccgcgcg gtgcagctgc actacatcac ccaggcgccg ggttcgcccc
     7261 cgcgcttcgt cgtcttcggc aacgggcgcc gcatcactcc cgcgtaccgc cgcttcgtgg
     7321 agcatcggct gcgcgaccgg ctcggcctca cgcacgcgcc gattacattg ctgttccgtc
     7381 gcaaaccatc gcgttgaccc ccgtgggggc ggatgctata ttccccgcgc ctccggagcg
     7441 cggatggtcc ttttcaacta ctcgacccgc gaacttaccg ccaaaatcgt ctactacggc
     7501 cccggtctct gcgggaagac caccaatctc cagttcatct acggctcgct ccccgacaac
     7561 gtcaagaagg ggaagatgct gtcgctggcg accaagaccg atcgcaccct gttcttcgac
     7621 ttcctgccga tcgagctggg gacgatccgc ggcatgcgca cgcgcgtgca gctgtacacc
     7681 gtgccgggcc aggtgttcta caactcgacg cgcaagctcg tgctcaaggg ggcggacggc
     7741 gtcgtgttcg tcgccgactc gcaggcgaag atgctcgagg cgaacaccga ggcgtaccgc
     7801 aacctccagg agaacctcaa ggagatgggg atccgcatcg aggagatccc gctggtcatg
     7861 cagttcaaca agcgcgacct cccgaacctc tgctccatcg aggagatgaa cacgcgcatc
     7921 aaccgccaca acgcgccgtt ctacgaggcg gtggcgatga ccggcatcgg cgtcgaggac
     7981 accctcaagg cgatcaccaa gctggtgctc aacaacctct cggccaagta ccgcctcgac
     8041 gaggccggcg cgacgcgccc cgccggagcc cccgccccgg cgccggtccc cgcgccgcgc
     8101 cgcgcccccg acccggtccg cgtggccccg cccgaggacc tcgcggtcga cgcgctcgac
     8161 gccggcacgc cccttcacga tcgccgcctg ccgtccaggc cggcgcgtcc cgtcatggcc
     8221 gagccggagg aggacgtcat cgagctgacc gacgagatcg gcggcacgga cgcgaccgac
     8281 gagccgttcg tcctggagcg cctgccggag cggccgcagc cggcggccgc cagcgtcacc
     8341 gtgcacccgg cggccatgcg ccccgcgctc gggccggcca cggcgcgcgc cgtggccccg
     8401 acgccgatgc tggcggagac cgatcccttc gacgacagcg atccgatcga cctggaggac
     8461 ccgctcgagg aaccgatcgc gtcggcaccg gtc
//
LOCUS       DASRIQ010000076         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_550559, whole genome shotgun sequence.
ACCESSION   DASRIQ010000076 DASRIQ010000000
VERSION     DASRIQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550559"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFD06_01940"
     CDS             complement(<1..798)
                     /locus_tag="VFD06_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN02324.1"
                     /translation="MAALPSAGVARGLIRILILVVVVLAIPVAGVFALLHAASQPPPD
                     QAKADPSEVLLQTEDATFVSSDGVSLSGWFLRGKRGVPPIVLCHDLGGSRSQLLNAAV
                     ALSKAGNPLLLLDFRRHGDSGIARSTLGVNERLDVLAAIGWLRGRPEFAQSRIGGWGV
                     GMGAYALALAALEEPGLTALALDGLYPDVATEADRRLKAMMPSAAQSLVPAARLLYGS
                     YFRCRLGQFALNHRLSGLSGRSLLLIAASEVPEQFEEERRIYDAIPET"
     gene            complement(810..2675)
                     /gene="uvrC"
                     /locus_tag="VFD06_01945"
     CDS             complement(810..2675)
                     /gene="uvrC"
                     /locus_tag="VFD06_01945"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZN02325.1"
                     /translation="MDSETNKGPERGTAGEAERPVRGKLADLPEAPGVYLYRDRRGRL
                     LYVGKAKSLRSRVRSYFQTGAPDEAKTGALVGEIWDLEYIVTRSDVEALILENNLVKE
                     EQPRYNIRLRDDKNFPYLKLTTSERFPRLVLVRQARLDKNAYYGPYLPASSARRTIQL
                     VARHFKVATCYEHLDGTRPRPCLLFQLNQCLGPCAGLVSDEEYRKAVADARLFLEGRT
                     RDLLGILKGKMTEAASDERYEAAAQHRDLIRTLERMQERQTISSVGLEGQDYVAYVRE
                     GGLASVQVFQMREGQVRARRELSFEGGEPDPGLFLDAVLERYYGSTDDLPETIVLPHE
                     PASPPVLEEWLRSRRGGRVRFLVPQRGPRRALLDTVAKNARISFEAMFRAPHTHGVEI
                     LEGLQEALGLDEPPHRIECFDISHIQGTDQVASLVVWEAGRPKRSDYRRFKIKTVEGN
                     DDFASMAEVVGRRYARLLREGKTLPDLVLIDGGKGQLSSALAVLEGLGLGHLQVAALA
                     KREEEIFLDGRAGPVRLPRDSPVLHLVQRIRDEAHRFAITFHRKVRSKRTVGSALTEI
                     EGVGPRRARQLLRRFGSIQGVREAPVEELGKVVGAAVARRVHQALVSTIPGERVV"
     gene            2744..>3343
                     /locus_tag="VFD06_01950"
     CDS             2744..>3343
                     /locus_tag="VFD06_01950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN02326.1"
                     /translation="MRRFADTAVALLLPSGARVARYFLDVAGFLYPHEGAFLHRLARA
                     APGRGTVVEIGSFHGRSTLCLASGIHRRGEGRVIAIDPQLKYGAGGSLAANLRRFGLE
                     GVVEVRAETSVAVARGFDGKARIVFVDGDHADEAVRADVAAWLPHLEPGGFLVLHDAT
                     SLSGFPGPRALASALQRTVGRDGTFQAAGTIGSIAWFRVQ"
BASE COUNT          468 a         1194 c         1189 g          492 t
ORIGIN      
        1 ggtctccggg atcgcatcgt aaatgcgccg ctcctcctcg aactgctccg gcacctccga
       61 tgcggcgatg agcaggaggc tcctcccgct cagaccggac aaccggtggt tcagggcgaa
      121 ctgcccgagc ctgcagcgga agtaggaacc gtacagcaaa cgggccgccg ggaccagcga
      181 ctgggccgcc gacggcatca tcgccttcag gcgccggtcg gcttccgtgg cgacgtccgg
      241 atacagaccg tcgagggcga gcgccgtcag gcccggctcc tcgagggcgg cgagggccag
      301 cgcgtacgcc cccatcccga ccccccagcc gccgatccgg ctctgcgcga actccgggcg
      361 cccgcgcagc cagccgatcg ccgccagcac gtcgagccgc tcgttcaccc cgagcgtcga
      421 gcgggcgatc ccgctgtcgc cgtgccgccg gaaatccagg agcaggagcg ggttccccgc
      481 cttgctgagg gccaccgcgg cgttgaggag ctgcgagcgc gatccgccga ggtcgtggca
      541 gagcacgatc ggcggcacgc cccgtttgcc gcgcaggaac cagccggaga gcgagacgcc
      601 gtcggacgac acgaaggtcg cgtcctcggt ctggagcagc acctcggaag ggtcggcctt
      661 ggcctgatcg gggggcggct ggctggccgc gtggaggagc gcgaagacgc cggcgaccgg
      721 gatcgccagc acgaccacca cgaggatgag aatccggatc agaccgcgag caacaccggc
      781 ggaggggagg gcggccatgc ggggcgcgac tataccacgc gctctccggg gatcgtggag
      841 accagggcct ggtgcacgcg gcgggcgacc gcagccccga cgactttccc cagctcctcc
      901 accggcgcct cgcgcacgcc ctggatcgac ccgaagcggc gcagcaactg gcgcgcgcgc
      961 cgcggaccca ccccttcgat ctcggtgagg gcgctgccca ccgtgcgctt gctgcgcacc
     1021 ttgcggtgga aggtgatggc gaagcggtgc gcctcgtcgc ggatgcgctg caccaggtgc
     1081 agcaccggcg agtcgcgcgg cagccgcacc ggcccggcgc ggccgtcgag gaagatctcc
     1141 tcctcccgct tggcgagggc ggcgacctgg aggtggccga ggccgagccc ctcgaggacg
     1201 gcgagcgcgg aggagagctg ccccttgccc ccgtcgatca ggacgaggtc agggagcgtc
     1261 ttcccttcgc gcagcagccg ggcgtagcgg cggccgacca cctccgccat cgaggcgaag
     1321 tcgtcgttcc cttccaccgt cttgatcttg aagcgccggt agtcggaacg cttcgggcgc
     1381 ccggcttccc agacgacgag cgacgccacc tgatcggtgc cctggatgtg ggagatgtcg
     1441 aagcactcga tgcggtgcgg cggctcgtcg aggccgagcg cctcctggag cccttcgaga
     1501 atctcgaccc cgtgcgtgtg cggggcgcgg aacatcgcct cgaacgagat gcgggcgttc
     1561 ttcgccacgg tgtcgagcag ggcgcgtctc gggccgcgct gcggcaccag gaaacggacc
     1621 cgcccgcccc gccggctccg gagccactcc tcgaggaccg ggggggacgc cggctcgtgc
     1681 ggcaggacga tcgtctccgg caggtcgtcg gtcgagccgt agtaacgctc cagcaccgca
     1741 tcgaggaaca ggccggggtc cggctcccct ccttcgaacg acagctcgcg ccgggcgcgc
     1801 acctgcccct cgcgcatctg gaacacctgc accgaggcga gcccgccttc gcggacgtag
     1861 gcgacgtagt cctgcccttc gaggccgacc gaggagatcg tctgccgctc ctgcatccgc
     1921 tcgagggtgc ggatgagatc gcggtgctgc gccgccgcct cgtagcgctc atccgacgcc
     1981 gcctcggtca tcttcccctt caggattccg agcaggtcgc gcgtccgccc ttcgaggaac
     2041 aggcgcgcgt cggcgaccgc cttgcggtac tcctcgtccg acacgagccc ggcacacgga
     2101 ccgaggcact ggttgagctg gaacaggagg catggccggg gccgcgtccc gtcgagatgc
     2161 tcgtagcagg tggcgacctt gaagtggcgc gccaccagct ggatcgtgcg gcgggcgctc
     2221 gatgccggca ggtacggtcc gtagtaggcg ttcttgtcga gccgcgcctg ccgcaccagg
     2281 acgaggcgcg ggaagcgctc ggaggtcgtc agcttcagat acgggaagtt cttgtcgtcc
     2341 ctgaggcgga tgttgtagcg gggctgctcc tccttgacga ggttgttctc gaggatgagc
     2401 gcctccacat cgctgcgcgt gacgatgtac tcgaggtccc agatctcgcc gaccagcgcc
     2461 ccggtcttcg cctcgtccgg cgccccggtc tgaaagtagg agcggacgcg gctccggagc
     2521 gacttcgcct tgccgacgta caggagccgc ccgcggcggt cccgatacag gtacacgccc
     2581 ggcgcttcgg gcaggtcggc cagcttgccc cggaccggcc gctcggcctc gcccgccgtc
     2641 ccgcgttccg gccccttgtt cgtctcggaa tccatcgctt cacgatctta ctctccgagg
     2701 catgggcccg cggcggcccg tccttccgtg agacgatggg cgcatgcgac gtttcgccga
     2761 cacggcggtc gcgctgctcc ttccctcggg ggcccgggtg gcgcgctact tcctcgacgt
     2821 cgcaggtttt ctgtacccgc acgagggggc cttcctgcac cggctggcgc gggccgcccc
     2881 cgggcgcggg acggtggtcg agatcggctc gttccacggc cgctcgaccc tgtgtctcgc
     2941 atcgggcatt caccggcgcg gcgaggggcg cgtgatcgcc atcgatcccc agctgaagta
     3001 cggcgccgga ggcagtctcg cggccaatct ccggcgcttc gggctcgagg gtgtcgtgga
     3061 agttcgggcg gagacttcgg tcgccgtcgc gcgagggttt gacgggaagg cgcggatcgt
     3121 gttcgtggac ggcgaccacg ccgacgaggc ggtgcgggcc gacgtcgctg cctggctgcc
     3181 gcatctggag cccggcggat tcctggtgct gcacgacgcg acctctctgt cgggatttcc
     3241 gggcccccgc gctctcgcca gcgccctgca acgcaccgtc ggccgggacg ggaccttcca
     3301 ggccgccggc acgatcggca gcatcgcctg gttcagggtc cag
//
LOCUS       DASRIQ010000077         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2370929, whole genome shotgun sequence.
ACCESSION   DASRIQ010000077 DASRIQ010000000
VERSION     DASRIQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2370929"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2161)
                     /locus_tag="VFD06_01955"
     CDS             complement(<1..>2161)
                     /locus_tag="VFD06_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN02327.1"
                     /translation="QIGVCTVGVCFNNFVSPTPTIGQACATHADCYVDIDRDADGILD
                     ALDNCVLTANGPLAGPSNQVDTDADGLGDACDGDCAGTVVVGRCRANGAICPNPGTSQ
                     PVACANALGLGDFCQRSLANSGGCSANGDDLDADGVLDAVDNCPTVANPPVYAGGPQR
                     DRDRDGLGDSCDPAAAHDDLPDGLPDDVVTFSGTLACHTRPLARLTLIDYPVYLDVDG
                     DHDQFPDTGERGRFTIVVRNDGPTLENAVLTLVSSDPDVACISESQIVVPSLPAGATM
                     TLGSLDPGQPGFGFVASDALQSQPLPAPSRIDLQIQVAGPGLLGLAAPLDVSLLADYD
                     MPPGTPQVYVTGPDGMPGTADDGKAVENFDVDRNGDGSITVRDTFLQAIAPGVFRGSC
                     SNAPLTVCATAADCPASPPGAVCYSGAYVRASATGSELNRVAAVSCGGFETATNYPAC
                     ALDPDFPMDWHLHCPPGATSCPNVESGTCVGGCSFGTPTGGQRALSGGNSLHMGAHFV
                     PGDNLAGDTTHLRALQGFMSAPLNLALLPRPGDLDLSFFHIARLMDNNGVGPNNENQC
                     ADCADVQIQVDQDPDPAVDAWGFWDKLAPYQNVYDHKPNAWSVFGGYYCVFTPTDTGT
                     APPNPRGVHETICYPQGAWSSCGSTIGLTSASTGDCAGPGSVDPSGVGVWVQTRFHLD
                     GYLGRRVRVRWIAESWAFDEHSSSYYQIGGTWAATQQ"
BASE COUNT          317 a          723 c          801 g          320 t
ORIGIN      
        1 tcctgctgcg tcgccgccca ggtgccgccg atctggtagt acgaggagct gtgttcgtcg
       61 aaggcccacg actcggcgat ccagcggacg cggacgcgcc ggccgagata gccgtccaga
      121 tggaagcgcg tctggaccca cacccccact cccgacggat cgaccgaccc cggccccgca
      181 cagtcgccgg tggaggcact ggtgaggccg atggtcgagc cgcagctgga ccacgccccc
      241 tgcgggtagc agatcgtctc gtggacgccg cgcgggttcg gcggcgccgt gccggtgtcg
      301 gtcggcgtga acacacagta ataaccgccg aagaccgacc aggcgttcgg cttgtgatcg
      361 tagacgttct ggtagggcgc cagcttgtcc cagaagcccc aggcatcgac ggccggatcc
      421 ggatcctggt ccacctggat ctgcacgtcg gcgcagtcgg cgcactggtt ctcgttgttc
      481 ggaccgaccc cgttgttgtc catcagccgc gcgatgtgga agaaggacag gtccaggtcc
      541 cccgggcgcg gcaggagggc gaggttcaag ggagcgctca tgaacccctg cagcgcacgg
      601 aggtgggtcg tgtcacccgc caggttgtcg ccggggacga aatgcgcccc catgtgcagc
      661 gagttcccac cgctcagggc gcgctggccg ccggtgggtg tgccgaacga gcagccgccc
      721 acgcaggtcc ccgattccac gttggggcag ctggtcgcgc cgggcggaca gtgcaggtgc
      781 cagtccatcg gaaagtccgg gtcgagggcg cacgcgggat agttggtcgc ggtttcgaag
      841 ccgccgcaac tcacggccgc gacccggttg agctcggagc cggtggcgct ggcgcgcacg
      901 tacgcccccg aataacagac cgcccccggc gggctggcgg ggcagtcggc ggccgtggcg
      961 caaaccgtga gcggcgcgtt gctgcacgag ccgcggaaca cgccgggggc gatcgcctgg
     1021 aggaaggtgt cgcgcaccgt gatgctgccg tcgccgttcc gatcgacgtc gaaattctcg
     1081 accgccttgc cgtcgtcggc ggtccccggc atcccgtccg gaccggtcac gtagacctgt
     1141 ggcgtcccgg gcggcatgtc gtagtcggcc aagagcgaga cgtcgagcgg cgccgcgagg
     1201 ccgagcaggc cgggcccggc cacctggatc tggaggtcga tgcgcgacgg cgccggcagc
     1261 ggctgcgact gcagcgcatc gctcgcgacg aagccgaacc cgggctggcc gggatcgagg
     1321 ctgccgagcg tcatggtggc gccggccggc aggctcggca cgacgatctg cgattcgctg
     1381 atgcaggcga cgtccgggtc gctcgacacg agcgtcagga cggcgttctc gagggtcgga
     1441 ccgtcgttgc gcacgacgat ggtgaagcgc ccgcgctccc cggtgtcggg gaactggtcg
     1501 tggtccccgt cgacgtcgag atagaccggg tagtcgatca gggtcagccg cgccagcggc
     1561 cgggtgtggc aggcgagcgt cccggagaag gtcacgacat cgtccggcag tccgtcgggg
     1621 aggtcgtcat gggccgccgc cggatcgcag gagtccccga gtccgtcccg atcgcgatcg
     1681 cgctgcggcc ctccggcgta gaccggcgga ttcgcgaccg tcgggcaatt gtcgaccgcg
     1741 tccaggacac cgtcggcgtc gaggtcgtcg ccgttcgcgg agcagccgcc ggagttggcg
     1801 agactgcgct gacagaagtc tccgaggccg agcgcgttgg cgcacgccac cggctggctc
     1861 gtcccgggat tcgggcagat ggcgccgttg gcgcggcagc gccccacgac caccgtcccg
     1921 gcgcaatcgc cgtcgcacgc atctccgagt ccgtcggcgt cggtgtcgac ctggttgctc
     1981 ggtccggcca gtgggccgtt cgccgtcagg acgcagttgt cgagcgcgtc gaggatcccg
     2041 tcggcgtcgc ggtcgatatc gacgtagcag tcggcgtgcg tcgcgcaggc ctgaccgatt
     2101 gtcggtgtcg gggagacgaa gttgttgaag cagacgccca cagtgcagac gccgatctgg
     2161 c
//
LOCUS       DASRIQ010000078         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1081427, whole genome shotgun sequence.
ACCESSION   DASRIQ010000078 DASRIQ010000000
VERSION     DASRIQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081427"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VFD06_01960"
     CDS             complement(<1..553)
                     /locus_tag="VFD06_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN02328.1"
                     /translation="MTTTRFLNLRDAIALVPDGALLSFGGFDMQRPPLALAAELVRQG
                     RKRLRVVVPPTPLPLDLLVGAGVVEEAEFGFIGFQYEEGFVVAPNVRRAVESGSLRWR
                     ERDVFEIVQGLRAAAAGVAYLPAPGGEGTDYERHWPAPQGKIDATGSGVATAAGAIAP
                     AIAPDFVLLHAQEADRHGNLSIAD"
     gene            complement(550..960)
                     /locus_tag="VFD06_01965"
     CDS             complement(550..960)
                     /locus_tag="VFD06_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HZN02329.1"
                     /translation="MELPRFHRLQGAYYRLEGSRCKGCDAGQFPPRRVCPKCGARDLE
                     SQRFSGRATLYAATRVTQAPRGHGSLAPYAVALVRLEEGPLVAAQLTDVGDGTLPAGL
                     PLEMVTRKIRDAEEHGLIVYGYKFRPRLEEPKRP"
     gene            complement(989..2158)
                     /locus_tag="VFD06_01970"
     CDS             complement(989..2158)
                     /locus_tag="VFD06_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434068.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HZN02330.1"
                     /translation="MRDVGVLGIGQTPVGEHWDRSIRDLAVDAARAALDEAGLDRVDA
                     IYVGNMASGLLCGQENLATLVADAAGLLPAEAVKIEAACASGGAAVRAGFQAIAGGLH
                     QRVLVVGVEKMTDRVTDGVTAALATAADADYEAAHGASFVALNAILMRRYLDERRIGR
                     EAFAPFALLAHKNAARNPHAFFRQEITAEEFARSPSVAPPIAVLDSAGICDGAAALVL
                     APLEECRRRGSEARRPSAVRLAASASVTDTLALGDRRDPLVWESVAGSARKALKEAGI
                     GLKDLSFYEPHDAFTIVTALSLEACGFADRFAAVDLAKDGRFDAGGELPICTLGGLKG
                     RGHPVGASGTYQVVEAVLQLRGAAGDAQVQGARLGMTQSVGGSGSVALTHVLEAV"
     gene            complement(2181..>2898)
                     /locus_tag="VFD06_01975"
     CDS             complement(2181..>2898)
                     /locus_tag="VFD06_01975"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HZN02331.1"
                     /translation="CKAGTEAMRAAAAFVGAGLTEAALAIGMDAAQGKPGDHLEYTAA
                     AGGGAVIVGPVSDQAIARIEGSCSYVTDTPDFFRRQHLHYPEHGHRFTGEPAYFRHVE
                     EASKRLLGTLGRAPSDYRFAVFHQPNPKFVRKAAAALGFTEAQVGPTAVVDRIGNTYS
                     GASLLGLAAALDLARPGDRIFLCSYGSGAGSDAFSFTVTDRIEAVRGRAPLLRAHLDR
                     RRPIEDYGTYLRRAGAVRTL"
BASE COUNT          405 a         1078 c         1047 g          368 t
ORIGIN      
        1 gatcggcgat ggagaggttg ccgtggcggt ccgcctcctg cgcgtgcagc aggacgaaat
       61 cgggggcgat cgccggcgcg atggcgcccg ccgcggtggc aacgccggat cctgttgcgt
      121 cgatcttccc ctggggcgcg ggccagtggc gctcgtaatc ggtcccctcc ccgcccggcg
      181 ccggcaggta ggccacgccg gccgcggcgg cccgcagccc ctggacgatc tcgaagacgt
      241 cccgctcgcg ccagcgcagg ctcccggact cgacggcgcg cctgacgttc ggcgcgacaa
      301 cgaaaccctc ttcgtactgg aagccgatga agccgaactc cgcctcctcc acgacccccg
      361 cgccgaccag gaggtcgagc ggcagcggcg tcggcggcac gacgacgcgg aggcgcttcc
      421 tgccctggcg gacgagctcg gccgcgagcg ccagcggcgg gcgctgcatg tcgaatccgc
      481 cgaaggacag aagcgccccg tcggggacga gagcgatggc atcgcgcagg ttcaggaagc
      541 gtgtggtggt catggacgct tgggctcctc gagccgtgga cggaacttgt agccgtagac
      601 gatgagcccg tgctcctccg cgtcgcggat cttgcgggtg accatctcga gcggcagccc
      661 ggccggcagc gtcccgtcgc cgacgtcggt gagctgcgcc gccacgagcg gcccttcctc
      721 cagacggacc agcgccacgg cgtaaggggc gagcgatccg tggccgcgcg gcgcctgcgt
      781 cacccgcgtc gcggcgtaga gcgtcgcgcg tcccgagaag cgctgcgact cgagatcgcg
      841 cgccccgcat ttcgggcaga cgcgccgcgg cgggaactgg ccggcgtcgc agcccttgca
      901 gcggctcccc tccagccggt agtaggcccc ctgcagccgg tggaaccgcg gcagctccat
      961 cagcctctct ccccggacgc cgtcctcgtc acaccgcctc caggacgtgg gtgagcgcga
     1021 ccgagccgga tccgccgacg ctctgggtca tgccgaggcg cgccccctgc acctgcgcgt
     1081 cgccggcggc accccgcagc tgcagcaccg cctcgaccac ctggtaggtg ccgctggcgc
     1141 ccaccggatg accgcgtccc ttgagaccgc cgagcgtgca gatcggcagc tccccgcccg
     1201 catcgaagcg gccgtccttc gcgagatcga cggcggcgaa ccggtcggcg aagccgcacg
     1261 cctcgaggct cagggcggtg acgatggtga aggcgtcgtg aggctcgtag aaactgagat
     1321 ccttgaggcc gatccccgcc tccttcagcg ccttgcgcgc ggagcccgcg accgactccc
     1381 agaccagcgg atcgcgccgg tcgccgagcg ccagcgtgtc ggtgaccgag gcgctcgcgg
     1441 cgaggcgcac ggcggacggg cgccgcgcct ccgagccccg ccggcggcat tcctcgagag
     1501 gcgccaggac cagggccgcc gcgccgtcgc agataccggc cgagtcgagc acggcgatcg
     1561 gcggcgccac cgaaggcgaa cgtgcgaact cctcggcggt gatctcctgc cggaagaagg
     1621 cgtgcggatt cctcgcggcg ttcttgtgcg ccaggagcgc gaacggggcg aacgcctcgc
     1681 ggccgatgcg gcgctcgtcg aggtagcgcc gcatcaggat ggcgttgagg gcgacgaagg
     1741 aggcgccgtg cgccgcctcg tagtcggcgt cggcggcggt ggcgagggcc gccgtcactc
     1801 cgtcggtgac gcggtcggtc atcttctcga ccccgaccac gagcacacgc tggtgcaggc
     1861 cgcccgcgat cgcctggaat ccggcgcgca ccgccgcccc gccggaggcg caggccgcct
     1921 cgatcttcac cgcctccgcc ggcaggagcc cggcagcgtc ggcgaccagg gtcgcgaggt
     1981 tctcctgacc gcacaggagc cccgacgcca tgttgccgac gtagatcgcg tccacgcggt
     2041 cgagacccgc ctcgtcgagg gcggcgcggg ccgcgtccac ggcgagatcg cggatcgagc
     2101 ggtcccagtg ctccccgacc ggcgtctggc cgattccgag cacgccgaca tcgcgcatca
     2161 gcatccgctc ccggcccgcg tcacagcgtc ctcacggcgc cggcgcgccg cagataggtg
     2221 ccgtaatcct cgattggacg gcgccggtcg agatgcgccc tgaggagcgg ggcccgtccg
     2281 cgcaccgcct cgatccgatc ggtcaccgtg aaggagaagg cgtcgctccc ggcgccggag
     2341 ccataggagc acaggaagat ccggtccccc ggacgggcga ggtcgagcgc cgccgccagg
     2401 ccgaggagcg acgcgcccga gtaggtgttg ccgatccggt ccacgaccgc ggtcggcccg
     2461 acctgcgcct cggtgaaccc gagcgccgcc gccgccttgc gcacgaactt cgggttcggc
     2521 tggtggaaga cggcgaagcg gtaatcggac ggcgcgcgcc cgagggtccc gagcagccgc
     2581 ttcgacgcct cctcgacgtg ccggaagtag gccggctcgc cggtgaaacg gtgcccgtgc
     2641 tccgggtagt gcaggtgctg ccgccggaaa aaatcggggg tgtcggtgac gtaggagcag
     2701 gagccttcga ttctggcgat cgcctggtcc gagacgggac cgacgatcac cgccccgccc
     2761 ccggcggcgg cggtgtattc gaggtggtcg cccggcttcc cctgcgccgc gtccatgccg
     2821 atcgccagcg ccgcctcggt cagcccggcc ccgacgaacg cggcggcggc gcgcatcgcc
     2881 tcggtccccg ccttgcag
//
LOCUS       DASRIQ010000079         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3422255, whole genome shotgun sequence.
ACCESSION   DASRIQ010000079 DASRIQ010000000
VERSION     DASRIQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3422255"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFD06_01980"
     CDS             complement(<1..184)
                     /locus_tag="VFD06_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02332.1"
                     /translation="MATTMTATRAWHEEAVAALEERPGVGLLQDIPLSDHTYIGIGGR
                     AKVLIEPETIADLAWGI"
     gene            complement(218..1594)
                     /gene="murC"
                     /locus_tag="VFD06_01985"
     CDS             complement(218..1594)
                     /gene="murC"
                     /locus_tag="VFD06_01985"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZN02333.1"
                     /translation="MLRKTRAIHFVGIGGSGMSGIAEVLVNLGYQVTGSDLEEGATVQ
                     RLRGLGATIARGHKAEHVGDADVVVVSSAVKPDNPEVQEARRRQIPVIPRAEMLAELM
                     RLKYGVAIAGSHGKTTTTSMVATVLSGAGLDPTIVIGGRLNILGSNAKLGRGDLLVAE
                     ADESDGSFLRLTPTIAVVTNIDAEHLDHYGSLTALQQAFTDFLNKVPFYGCGIVCLDE
                     PRVQEILPALTRRVVTYGFSAQADLSAASVDLKEFHAAFDVRMHGKKLGRARIQVPGR
                     HNVSNALAAIAVGLDLDVPFATVTTQLESFRGADRRFHLKGEAKGIMVIDDYGHHPVE
                     ILATLSAAKKGWNRRTVVVFQPHRYSRVQALANEFARAFYDADVLAVTDIYPAGEAPI
                     AGVTSEAVAHAIEEHGHKDVTLVKDIKAVPDWLVERAREGDMVITLGAGSIWKAGEEY
                     LRRIKGGA"
     gene            complement(1597..>2544)
                     /gene="murG"
                     /locus_tag="VFD06_01990"
     CDS             complement(1597..>2544)
                     /gene="murG"
                     /locus_tag="VFD06_01990"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZN02334.1"
                     /translation="HGYALVAIRSGGVVGRSLFGRLAGIGNALRGFFEAGKVIDILKP
                     QAVIGVGGYASFPMVLAAQARGVPTLIQDQNAVPGLSNRLLGRSARAIALTYDETRRF
                     FAGRGTVTGNPIRAEFHRADREQARRRRASGEPRRHLFVFGGSQGSRALNDAVLQALP
                     HLRERRDRLRIVHGTGPADADRMRAAYADHGLDATVLPYLDDIMGAYEEADLVVSRSG
                     ASTVSELAACGKAAILVPLPTSAHDHQKKNAEAASRAGAAVLLEQRDLTGATLAGEIV
                     RLLDDPGRLEAMERAAAGLARPDAAARIVELVLGLEGRP"
BASE COUNT          339 a          900 c          920 g          385 t
ORIGIN      
        1 cgatccccca cgccaggtcc gcgatcgttt ccggctcgat caggactttc gcccggccgc
       61 cgatgccgat gtaggtgtgg tcggagagcg ggatgtcctg gagcagaccg actccgggcc
      121 gctcttcgag agccgccacc gcctcctcgt gccacgcgcg cgtcgccgtc atcgtcgtcg
      181 ccatgccttc agatgacctc cttcactgcc ggaggcttca cgcgcccccc ttgatccggc
      241 gcaggtactc ctcgccggcc ttccagatgg agccggcccc gagggtgatc accatgtccc
      301 cctcgcgggc gcgctccaca agccaatccg ggaccgcctt gatgtccttc accagcgtca
      361 cgtccttgtg gccgtgctct tcgatggcgt gcgccaccgc ttcggacgtg accccggcga
      421 tcggcgcctc gccggccggg tagatgtcgg tgaccgccag cacgtcggcg tcatagaagg
      481 cgcgcgcgaa ttcattggcg agcgcctgca cgcgcgagta gcggtgtggc tggaagacga
      541 cgacggtgcg ccggttccag cccttcttcg cggccgacag ggtggcgagg atctcgacgg
      601 gatggtggcc gtagtcgtcg atcaccatga tgcccttcgc ctcccccttg aggtggaagc
      661 ggcggtcggc gccgcggaac gactcgagct gcgttgtgac ggtggcgaac ggcacatcga
      721 ggtcgagccc gacggcgatg gccgcgagcg cgttcgagac gttgtgccgc cccggcacct
      781 ggatgcgggc ccggccgagc ttcttgccgt gcatgcggac gtcgaaggcg gcgtggaact
      841 ccttgagatc gaccgacgcc gccgaaaggt ccgcctgcgc cgagaacccg taggtcacga
      901 cgcgccgggt gagcgccggg aggatctcct gcacgcgcgg ctcgtcgagg cagacgatgc
      961 cgcagccgta gaacggcacc ttgttgagga agtcggtgaa cgcctgctgc agggcggtga
     1021 gggagccgta gtggtcgagg tgctcggcat cgatgttggt gaccaccgcg atcgtcggcg
     1081 tcaggcgcag gaacgacccg tcgctctcgt ccgcctcggc gaccaggagg tcgccgcgcc
     1141 ccagcttggc gttgctcccg aggatgttga gccggccgcc aatgacgatg gtcgggtcga
     1201 gcccggcgcc ggagaggacc gtcgccacca tcgaggtggt ggtcgtcttg ccgtggctgc
     1261 cggcgatggc gacgccgtac ttcaggcgca tcagctcggc gagcatctcg gcgcgcggga
     1321 tgaccgggat ctgccggcgg cgcgcctcct gcacctcggg gttgtccggc ttcaccgccg
     1381 acgacaccac cacgacgtcg gcgtcgccga cgtgctccgc cttgtggccc ctggcgatgg
     1441 tggcgccgag gccgcggagg cgctggacgg tggcgccctc ctcgaggtcg ctgccggtga
     1501 cctggtagcc caggttcacc agcacctcgg cgatgccgct catgccggag ccgccgatgc
     1561 cgacgaaatg gatcgcccgg gtcttccgga gcatcatcac gggcgtccct ccagccccag
     1621 gacgagctcg acgatgcggg ccgccgcgtc cgggcgggcg agcccggcgg cggcgcgctc
     1681 catcgcctcg aggcgccccg ggtcgtcgag gagccggacg atctccccgg cgagggtcgc
     1741 gccggtgagg tcgcgctgct cgaggaggac cgccgctccg gcccgcgacg ccgcctcggc
     1801 gttcttcttc tggtgatcgt gggccgacgt cgggagcggc accaggatcg ccgccttgcc
     1861 gcaggcggcg agctcgctga cggtgctcgc ccccgagcgg gagaccacga ggtcggcctc
     1921 ctcgtaagcc cccatgatgt cgtcgaggta ggggaggacg gtcgcgtcga ggccgtgatc
     1981 cgcgtacgcc gcccgcatcc ggtcggcgtc ggcggggccg gtgccgtgca cgatgcgcag
     2041 gcggtcccgg cgctcgcgca ggtggggcag cgcctgcagc accgcgtcgt tgagggcgcg
     2101 cgaaccctgg ctgccgccga agacgaacag gtgccgccgc ggctccccgg aggcgcgccg
     2161 gcgcctggcc tgctcccggt cggcgcggtg gaactccgcc cggatcgggt tgccggtgac
     2221 cgtcccgcgg ccggcgaaga agcgccgggt ctcgtcgtag gtcagggcga tggcgcgggc
     2281 ggagcggccg agcaggcggt tcgacagtcc cggcacggcg ttctgatcct ggatcagggt
     2341 cgggacgccg cgcgcctggg ccgccaggac catcgggaag gaggcgtacc ctccgacgcc
     2401 gatcacggct tgaggtttca ggatgtcgat cacctttccc gcctcgaaga atccccgcag
     2461 ggcgttgccg atccccgcca gccgcccgaa cagcgaccgc ccgacgactc cgccgcttcg
     2521 aatcgccacc agcgcgtacc cgtg
//
LOCUS       DASRIQ010000080         8722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_553364, whole genome shotgun sequence.
ACCESSION   DASRIQ010000080 DASRIQ010000000
VERSION     DASRIQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553364"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFD06_01995"
     CDS             <1..327
                     /locus_tag="VFD06_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZN02335.1"
                     /translation="DEDAEAGAAVAFLRHPATMAAVTAERAFLAALGGGCRAPIGALA
                     EIEGGRLRLRGAVIDPSGRRLEAGERTGDRSGAAEIGRALAADLLDRGAVEMLARLRA
                     APFGSA"
     gene            327..1175
                     /locus_tag="VFD06_02000"
     CDS             327..1175
                     /locus_tag="VFD06_02000"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZN02336.1"
                     /translation="MPTARRPLHGRTIVVTRATEQAGALARRLRGAGARVLEAPAIAF
                     APPRSWSAADRALEGLDRFDLLLFTSVNGVVRFLERARRRSGRREALRRIPVVAIGPA
                     TAAAARRGGLRVRAVPRQFRAEGLVRLLRGLGRHRAAEAPSRGHSASAARPRGLGPAR
                     ILMPRAAAGRDVVLRALRRRGARVTVIPVYRTVPSRRGRAPVARALRTGRIDLVTFAS
                     AATARHFAAAFAGPDRRRLRRVPAAVIGPITAREARRLGFRIAAMPARATIPDLARAI
                     ARALRS"
     gene            1258..2397
                     /gene="gcvT"
                     /locus_tag="VFD06_02005"
     CDS             1258..2397
                     /gene="gcvT"
                     /locus_tag="VFD06_02005"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZN02337.1"
                     /translation="MTSDAAAGPLKTTPLHAIARSLGGKLVPFAGWEMPVQFSSVIEE
                     HLAVRTAAGIFDVSHMGEIEVEGSGALALVQRLTCNDVSRLADGQAQYSGLLNEAGGF
                     VDDILVYRRAAQRYLLVVNAGNTAKDVAWVARHAKEIRDVEARDASARYAQIALQGPR
                     AEAILQGLVKTDLRGIRYYHFAEADVRGARSIVSRTGYTGEDGFEIYGPAEAAEDLFR
                     GILEAGRPHGLLPCGLGARDTLRLEARMPLYGNDLDDTTTPLEAGLGGIVKLAKGEFT
                     GRAALETQKREGVARQLAGFEMVDRGIARHDYPVRHEGTTIGQVTSGTFAPYLKKNIG
                     LAYVPPAHAAVGSRFSVVIRERDAAALVVETPFYRRPPRVSGRSH"
     gene            2408..2809
                     /gene="gcvH"
                     /locus_tag="VFD06_02010"
     CDS             2408..2809
                     /gene="gcvH"
                     /locus_tag="VFD06_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZN02338.1"
                     /translation="MSADGIYPDDLKYTKEHEWLEARGTQATVGVTHYAQAELGDIVY
                     VELPQVGAAVVAGEAFGTVESVKAVSELFAPASGTVAEVNAALAEKPDAVNRDPYGDG
                     WMIRITMADPKEVDGLMSASQYRKYIEDEAN"
     gene            2809..4164
                     /gene="gcvPA"
                     /locus_tag="VFD06_02015"
     CDS             2809..4164
                     /gene="gcvPA"
                     /locus_tag="VFD06_02015"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZN02339.1"
                     /translation="MSGVHRYLPLSEAERAEMLRAAGFDRPEDLFASIPAGLRLKGAL
                     DVPGPLAEQDLLADFKERAADNRVPDPRAQFLGGGAYRHYQPSIVDHLISRTEFYSSY
                     TPYQPEISQGTLQAVYEYQTLVCQLTGLDLSNASMYEGASSVAEAILMADRVSPKKRA
                     VLSDRLHPEYALTVRTYLAHVGIEVATFGHRPDGTADPQAAVQALGAGASALVVQHPN
                     FFGCVEEIEALAAAAKKAGAQLIVAVTEPVSLGLLKPPGAQGADIVVGEAQGLGVPLS
                     FGGPFLGFLAARSAFMRHMPGRLVGEAKDVDGRRGYVLTLSTREQHIRREKATSNICT
                     NEGLCALTASIFMATLGKRGMMDLARHNHAKAAYARGRLASVKGLSFPEPAPFFNEFV
                     VRLKGSAGEAVQRLAGKGILAGIPLSRYFRDRERDLLVCVTEMNPKGEIDRLADEIGR
                     L"
     gene            4164..5639
                     /gene="gcvPB"
                     /locus_tag="VFD06_02020"
     CDS             4164..5639
                     /gene="gcvPB"
                     /locus_tag="VFD06_02020"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZN02340.1"
                     /translation="METRTLRPAEPLLFEVSRPGRRGADLPPLDVPAEGPHHLDRRHL
                     RDDIEGFPELSEVDVVRHYTRLSRLNYAIDLGLYPLGSCTMKYNPKINEKVARLPGFA
                     DLHPQTPEEMSQGALKLMHHLERALAAICGMDRFTLQPAAGAQGEQVGILMVRAYHQD
                     RGNPRQVVLIPDSAHGTNPASAHLAGYRVEQVASNARGCVDLEALRRRVGDDVAVLML
                     TNPNTLGVFEGEIEAVARTLHDRGALLYMDGANLNAFVGQARPGDMGVDVHHLNLHKT
                     FSTPHGGGGPGAGPVGVKAILEPYLPAPVIERDGDRLRFHHDRPKSIGRIRSFHGNFG
                     MFVRAWAFILAHGPDGLKRMSETAVLNANYLRRRLETTWHLPYKEPSLHEVVFSDKNL
                     EATGVRTLDVAKRLMDYGFHPPTIYFPLIVKGALMIEPTETESREELDLFADALLKIE
                     AEARRDPELVKNAPYTTPIRRLDETAAARNPVLRWSPKAEN"
     gene            complement(5665..6033)
                     /locus_tag="VFD06_02025"
     CDS             complement(5665..6033)
                     /locus_tag="VFD06_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02341.1"
                     /translation="MESAVLEPYDKLIPVVINGRTYEVPENNTLLRVLQFMNLELAYT
                     KYCWNGDCRNCAFRYVSRRTGKEVEALGCQMRCFTNIQITRLPEGVHLPGEAPAGAAP
                     GNGGTPSVPAAGSGSTPAAA"
     gene            complement(6139..6753)
                     /locus_tag="VFD06_02030"
     CDS             complement(6139..6753)
                     /locus_tag="VFD06_02030"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZN02342.1"
                     /translation="MAEPTSVAAPGRLLVLASASPRRAELLSLLGLRFDVRPTNVDES
                     PRPGEAPADLAERLAREKALATTPSAGPSLVVAADTVVVLDGGLLGKPADRSEAAAFV
                     GRLAGRTHEVMTGLAVRALPEEALESEVVRSRVTFSPMRPDEIDWYARTGEGLDKAGA
                     YALQGIGAVFVRSVEGSYTNVIGLPMERLYPSLVRFGILRIAGS"
     gene            complement(6761..7033)
                     /locus_tag="VFD06_02035"
     CDS             complement(6761..7033)
                     /locus_tag="VFD06_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HZN02343.1"
                     /translation="MAKGGLRLGHLVVVVLLAALIGTATGDLLRTLWPESSSVAWLAE
                     GIRIGTNTPFDIDMVVAQLTFGAGIRLSLLGGLCAAFALFFFVRRV"
     gene            7132..7371
                     /locus_tag="VFD06_02040"
     CDS             7132..7371
                     /locus_tag="VFD06_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02344.1"
                     /translation="MIDGARRGLGRVVARAGALVALAALMALPLLGAAPATPSQGQSL
                     PGQPAPPLPTIASGPAPDLDLLFTAQVAGWIEPCG"
     gene            7531..8718
                     /locus_tag="VFD06_02045"
     CDS             7531..8718
                     /locus_tag="VFD06_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZN02345.1"
                     /translation="MNRIGYAAAAFGPRELNHGWEAFAARQKAMKFPLVSANIVWQDT
                     AQPVVQPYVIVKTALRRGAKNKEVRLGVLGLAAVNPAFLKTGPQNRRIVTVDPIAAAQ
                     KYAAELRSKADVVIALSSLDLESARNLARKVKEIDLLLGGNGAMQTRPDDFPEDSLFG
                     RTRLQYIGDQGKNVGEVRLLFGEKRALATVTRAVVGLTRDWPDEPELARLMEETKVAI
                     NEYHKGQAEASNPFAAAPVAPPSGSGAAAAAAPGGAVPPLVTARGAGAVSEYTGSERC
                     QPCHEQEYAVWTKSKHAHAFDILEKVHQDFNPTCVGCHVIGHGRTGGFVNAKATPGLK
                     HVGCEACHGPSSRHPDSIGHGYGVTSIVDCASCHTRDNSPDYDPGLYVPQVRHWGEAQ
                     AGH"
BASE COUNT         1265 a         3146 c         3127 g         1184 t
ORIGIN      
        1 gacgaggacg cggaggccgg cgccgcggtc gccttcctgc gccacccggc gacgatggcg
       61 gcggtgaccg cggagcgcgc cttcctcgcg gccctcggag gcggatgccg cgcgccgatc
      121 ggggcgctcg cggagatcga gggcgggcgg ctccggctgc gcggcgcggt gatcgatcct
      181 tcggggaggc gcctcgaggc gggggagcgc acgggagatc gctccggtgc ggcggagatc
      241 ggccgggccc tcgccgccga tctgctggac cgcggcgccg tggagatgct ggcgcgcctg
      301 cgcgccgccc ccttcggttc ggcctgatgc ccacggcccg gcggccgctt cacgggcgaa
      361 cgatcgtcgt cacgcgcgcg acggagcagg cgggggctct cgcccggcgg ctccgaggcg
      421 cgggtgcgcg cgtgctcgag gcccccgcca tcgccttcgc gccgccccgg tcctggagcg
      481 ccgccgatcg ggccctcgaa ggcctcgatc gattcgatct cctcctgttc acgagcgtca
      541 acggggtcgt gcgcttcctg gagcgggcgc gacggcgttc ggggcggcgc gaggcacttc
      601 gacggatccc ggtggtcgcc atcgggccgg cgaccgccgc ggcagcgcgg cgcggcgggc
      661 tgcgcgtgcg ggcggtgccg cggcagttcc gcgccgaggg gctcgtccgg ctcctgcggg
      721 ggctggggcg acatcgcgcg gcggaggcgc cgtcgcgcgg gcattccgca tcggccgcgc
      781 ggccgcgcgg cctcggcccg gcgcggatcc tgatgccgcg ggcggcggcg ggacgcgacg
      841 tcgtcctgcg ggcactgcgg cgccgggggg cccgcgtcac cgtgatcccg gtctaccgga
      901 ccgtgccgtc gcgccgcggc cgcgccccgg tcgcccgggc gctccggacc ggccgcatcg
      961 acctcgtcac cttcgcctcg gccgcgaccg cccggcactt cgcggccgcc ttcgcggggc
     1021 ccgatcggcg ccggctgcgg cgcgtgccgg cggccgtcat cggaccgatc accgcccgcg
     1081 aagcgcgccg gctcggcttc cgaatcgccg ccatgccggc gcgggcgacg attccggatc
     1141 tcgcccgcgc gattgcgcgc gccctgcgct cctaatgggt ccgcagcagt gcgtccggcc
     1201 ggaagccgcc ttcccacgct gtggtacagt acggcccctt cggagaactc ctcgagaatg
     1261 acctcggacg ccgcggccgg gccgctcaag accacaccgc tccacgcgat cgcccgctcg
     1321 ctcggcggga agctggtgcc gttcgcaggc tgggagatgc cggtgcagtt ctcctcggtg
     1381 atcgaggagc acctcgccgt gcgcaccgcc gccgggatct tcgacgtcag ccacatgggg
     1441 gagatcgagg tcgagggatc gggcgccctg gcgctggtgc agcggctgac gtgcaacgac
     1501 gtctcccgcc tcgccgacgg ccaggcgcag tactcggggc tcctgaacga agcgggggga
     1561 ttcgtcgacg acatcctcgt ctaccggcgt gcggcgcagc gctacctgct cgtcgtcaac
     1621 gccggcaaca ccgccaagga cgtggcctgg gtcgcgcggc atgcgaagga gatccgggac
     1681 gtcgaggcgc gcgatgccag cgcccgctac gcgcagatcg cgctccaggg cccgcgcgcc
     1741 gaggcgattc tccaggggct cgtgaagacc gatcttcggg gcatccgcta ctaccacttc
     1801 gccgaggcgg atgtgcgcgg cgcgcgctcg atcgtctcgc gcaccggcta caccggcgag
     1861 gacggcttcg agatctacgg cccggccgag gcggccgagg atctcttccg ggggattctg
     1921 gaggccggcc gaccccatgg gctcctgccg tgcgggctcg gggcgcgcga cacgctccga
     1981 ctcgaggcgc gcatgccgct gtacggcaac gacctcgacg acaccacgac cccgctcgag
     2041 gccgggctcg gcgggatcgt caaactcgcg aagggggagt tcaccggccg cgccgcgctc
     2101 gagacccaga agcgggaggg ggtggcgcgg cagctcgccg gcttcgagat ggtggaccgc
     2161 ggcatcgcgc gccacgacta cccggtgcgg cacgagggga cgacgatcgg ccaggtcacc
     2221 tccgggacct tcgcgccgta cctcaagaag aacatcgggc tggcctacgt gccgccggcg
     2281 cacgccgcgg tcggatcgcg cttctcggtc gtcatccggg agcgggacgc cgcggcgctc
     2341 gtggtcgaga ctccgttcta ccggcggccg cctcgggtga gcggccgatc ccactgagga
     2401 gggggctttg agcgcagacg gcatctatcc ggacgacctg aagtacacca aggagcacga
     2461 gtggctcgag gcccgcggta cgcaggccac cgtcggcgtc acccactacg cccaggcgga
     2521 gctgggggac atcgtctacg tcgagctgcc ccaggtcggt gcggcggtgg tggcggggga
     2581 ggcgttcggc accgtcgagt cggtgaaggc ggtctccgag ctgttcgcac ccgcctcggg
     2641 caccgtcgcg gaggtgaacg cggcgctcgc cgagaagccg gacgccgtca accgcgatcc
     2701 gtacggcgac ggctggatga tccgcatcac catggccgat cccaaagagg tggacggcct
     2761 gatgagcgcc tcccagtacc gcaaatacat cgaggacgag gccaactgat gtccggcgtg
     2821 caccgctacc tgccgctctc cgaggcggag cgggcggaga tgctgcgcgc cgccggcttc
     2881 gatcgtcccg aggatctgtt cgcctcgatt ccggcgggcc tgcgcctcaa gggggcgctc
     2941 gacgtcccgg gacccctggc ggagcaggac ctcctcgccg acttcaagga gcgcgcggcg
     3001 gacaaccggg ttccggaccc gcgcgcgcag ttcctcggcg gcggcgccta ccggcactac
     3061 cagccctcga tcgtcgacca tctgatctcg cgcaccgagt tctactcgtc ctacacgccg
     3121 taccagccgg agatctcgca gggaaccctt caggctgtct acgagtacca gacgctcgtc
     3181 tgccagctga ccggcctcga cctctccaac gcctcgatgt acgaaggggc ctcgagcgtc
     3241 gccgaggcga tcctgatggc ggatcgtgtg tccccgaaga agcgcgccgt actctccgat
     3301 cgcctgcatc cggaatacgc cctgacggtc cgcacctatc tcgcgcacgt cggcatcgag
     3361 gtggccacgt tcggtcaccg ccccgacggc acggccgacc cgcaggcggc ggtccaggcg
     3421 ctcggtgccg gagcgtcggc tctggtggtc cagcacccga acttcttcgg ctgcgtcgag
     3481 gagatcgagg cgctggcggc ggcggccaag aaggcggggg cgcagctcat cgtcgcggtg
     3541 accgagccgg tgtcgctcgg cctcctcaag ccgcccggag cgcagggggc cgacatcgtg
     3601 gtgggggagg cgcaggggct cggggtgccg ctgtcgttcg gcgggccgtt cctcggcttc
     3661 ctcgcggccc gctcggcctt catgcgtcac atgccggggc gcctcgtcgg cgaggcgaag
     3721 gacgtggacg gccggcgcgg ctacgtgctg accctgtcca cacgcgagca gcacatccgc
     3781 cgcgagaagg cgacctccaa catctgcacc aacgaagggt tgtgcgccct gaccgcctcg
     3841 atcttcatgg cgacactcgg caagcgcggc atgatggatc tcgcgcgtca caaccacgcg
     3901 aaggcggcct acgcccgcgg ccgcctcgcg tcggtgaagg ggctctcgtt cccggagccg
     3961 gcgccgttct tcaacgagtt cgtcgtgcgc ctcaagggat ccgccgggga ggcggtgcag
     4021 cgtctcgccg ggaaggggat cctggcgggg attccgctct ccaggtattt ccgcgaccgg
     4081 gagcgcgacc tcctcgtctg cgtcaccgag atgaacccga agggcgagat cgaccggctc
     4141 gccgacgaga tcgggaggct gtgatggaga cccgcaccct ccgccccgcc gagccgctgc
     4201 tgttcgaggt gtcgcgcccc ggacggcgcg gcgccgacct gccgccgctc gacgtgccgg
     4261 ccgaagggcc gcaccatctc gaccgccggc acctgcgcga cgacatcgag ggattccccg
     4321 agctgagcga ggtggacgtg gtgcgccact acacccgcct gtcgcgcctc aactacgcca
     4381 tcgacctcgg tctgtacccg ctcgggtcgt gcaccatgaa gtacaacccg aagatcaacg
     4441 agaaggtggc gcggctgccc gggttcgccg atctgcaccc gcagacgccg gaggagatga
     4501 gccagggggc gctgaagctc atgcaccacc tggagcgggc cctcgccgcc atctgcggca
     4561 tggaccggtt cacgctgcag ccggcggccg gagcgcaggg ggagcaggtc ggcatcctca
     4621 tggtgcgcgc gtaccaccag gaccgcggca acccgcgcca ggtggtcctc atccccgact
     4681 cggcgcacgg caccaatccg gcgagcgccc acctcgccgg ctatcgcgtc gagcaggtgg
     4741 cgtcgaacgc ccgcggctgt gtcgatctcg aggcgctgcg ccggcgggtc ggcgacgacg
     4801 tcgccgtcct gatgctgacg aacccgaaca ccctcggggt attcgagggg gagatcgagg
     4861 cggtcgcccg cacgctgcac gatcgcgggg cgctcctcta catggacggc gccaatctga
     4921 acgccttcgt cggtcaggcg cgccccggcg acatgggggt ggacgtccac cacctcaacc
     4981 tgcacaagac cttttcgacg ccgcacggcg gcggcggccc gggggcgggg ccggtcgggg
     5041 tgaaggcgat cctcgagccg tacctgccgg cgccggtgat cgagcgcgac ggcgatcggc
     5101 tgcgcttcca ccacgatcgt ccgaagagca tcggccgcat ccgctccttc cacggcaact
     5161 tcgggatgtt cgtgcgcgcc tgggcgttca tcctggcgca cgggccggac gggctcaagc
     5221 ggatgtcgga gaccgccgtc ctcaacgcca actacctgcg ccgccgcctc gagacgacct
     5281 ggcacctgcc gtacaaggag ccgtccctcc acgaggtggt cttctcggac aagaacctcg
     5341 aggcgaccgg ggtgcggacg ctcgacgtcg ccaagcgcct gatggactac ggcttccacc
     5401 cgccgacgat ctacttcccg ctcatcgtca agggggccct gatgatcgag ccgaccgaga
     5461 ccgagtcgcg ggaagagctc gacctgttcg ccgacgcgct cctcaagatc gaagccgagg
     5521 cgcgccgcga ccccgagctg gtgaagaacg ccccgtacac gacgccgatc cgccgcctcg
     5581 acgagacggc ggcggcccgc aatccggtcc tgcgctggag tccgaaagcc gagaattagg
     5641 aagtccggga agcgccgcgg acggctacgc ggcggccggg gtgcttccgc ttccggccgc
     5701 cgggacggac ggtgtcccgc cgtttcccgg cgccgcaccc gccggcgcct cccccggaag
     5761 gtggacccct tcggggagcc gggtgatctg gatgttcgtg aagcagcgca tctggcagcc
     5821 gagcgcctcc acttccttgc ccgtgcgccg cgagacgtag cggaaggcgc aattgcggca
     5881 gtcgccgttc cagcagtatt tcgtgtacgc cagctcgagg ttcatgaact gcaggacccg
     5941 gagcagcgtg ttgttctccg gcacctcgta ggtcctgccg ttgatcacga ccggaatgag
     6001 cttgtcgtac ggctcgagga cggccgattc caagcagagt gccccccacc gccccggcgg
     6061 gcggccgctc ccaatctatg tcccggctgc gcgggggggc aacgacgggg cggtctccgt
     6121 cgatcgaacc ggaccgcctc aggatccggc gattctcagg atgccgaagc ggacgaggga
     6181 agggtagagg cgctccatcg gcaggccgat gacattggtg taacttccct cgacgctgcg
     6241 gacgaacacc gccccgatcc cctggagcgc gtacgcgccc gccttgtcga gcccctcgcc
     6301 ggttcgcgcg taccagtcga tctcgtcggg gcgcatcggc gagaaggtga cccgcgagcg
     6361 gaccacctcg gactcgagcg cctcctcggg gagcgcccgc accgccaggc cggtcatcac
     6421 ctcgtgcgtc cggccggcga ggcgcccgac gaaggccgcc gcctccgacc gatcggccgg
     6481 cttgccaaga aggccgccgt cgagcaccac gacggtgtcg gcggcgacga cgagggacgg
     6541 ccccgcggag ggcgtcgtcg ccagcgcctt ctcccgagcc agacgctcgg cgagatcggc
     6601 cggcgcctct cccgggcgcg gggactcgtc cacgttggtc ggccggacgt cgaagcgcag
     6661 ccccaaaagc gacagcagct ccgcgcggcg cggcgaggcg gaggcgagca caaggagccg
     6721 accgggcgcg gcgacggagg tcggctcggc catcgtccga tcagacccgg cggacgaaga
     6781 agaacagggc gaaggccgcg cacagcccgc cgaggaggct gagccggatg ccggcgccga
     6841 aggtcagttg cgccacgacc atgtcgatgt cgaagggggt gttcgtgccg atccggatgc
     6901 cctcggcgag ccaggcgacc gaggaggact cgggccagag cgtccgcagc aggtcgccgg
     6961 tggcggtgcc gatgagggcg gcgagcagga ccaccaccac gagatgaccc agccggagcc
     7021 cccccttcgc cacgcgcgga ctctaccacg ccggtttgac cccctcggga gggaacccta
     7081 taatccccgc cgcgcatgcg tggcgcgggg ttcggcggag acgggggttt cgtgatcgac
     7141 ggcgctcgaa gggggttggg acgggtggtg gcgcgcgccg gcgcgctggt cgcgctcgcc
     7201 gctctgatgg ccctgccgct tctcggcgcc gcccccgcca ccccttcgca gggacagagc
     7261 ctcccggggc agccggcgcc gccgctcccc acgatcgcct ccggtcccgc ccccgatctc
     7321 gatctgctgt tcaccgctca ggtggccggc tggatcgagc cctgtggctg accgcacaac
     7381 ccgcgcggcg ggctggcccg gcgggctggc tacaagacgg ttttcggcaa gtcgtatccc
     7441 gacacgccga tcgtctggct cgacgcgggc gatttcaccg gcgatccgac cgtcgccggg
     7501 gagaggcaga ccgacgccct catcgagggg atgaaccgga tcggctatgc ggcggccgcc
     7561 ttcggtccgc gcgagctgaa ccacgggtgg gaggcgttcg ccgcccggca gaaggcgatg
     7621 aagttcccgc tcgtctccgc caacatcgtc tggcaggaca ccgctcagcc ggtcgtccag
     7681 ccgtacgtga tcgtcaagac ggcgctgcgg cggggcgcga aaaacaaaga ggtccggctc
     7741 ggcgtcctcg ggctcgcggc cgtgaacccg gcgttcctga agacgggacc ccagaaccgg
     7801 cggatcgtga cggtcgatcc gatcgcggcg gcgcagaagt acgccgccga gctgcgctcc
     7861 aaagcggacg tcgtgatcgc gctctcatcg ctcgacctcg agtcggcccg caacctcgcg
     7921 cgcaaggtga aggagatcga tctgctgctc ggcggcaacg gcgccatgca gacccgtccg
     7981 gacgacttcc ccgaggattc gctgttcggg cggacccggc tgcagtacat cggcgaccag
     8041 gggaagaacg tgggcgaggt gcggctcctc ttcggcgaga agcgagccct cgccacggtc
     8101 acgcgcgcgg tcgtcgggct gacccgcgac tggccggacg agccggagct cgcgcgactg
     8161 atggaggaga cgaaagtcgc catcaacgag taccacaagg gacaggcgga ggcgtcgaac
     8221 ccgttcgccg cggccccggt cgcccccccg tccggctccg gcgcggcagc ggccgccgcc
     8281 ccgggcggcg ccgtgcctcc gctggtcacc gcgcgcggcg cgggcgccgt ctccgagtac
     8341 accggctcgg agcgctgcca gccgtgccac gagcaggagt acgccgtctg gacgaagtcg
     8401 aagcacgcgc atgccttcga catcctcgag aaggtgcacc aggacttcaa tccgacctgc
     8461 gtcggctgcc acgtcatcgg ccacgggagg accggtgggt tcgtcaacgc caaggcgacg
     8521 ccgggactga agcacgtcgg gtgcgaggcc tgccacggtc cgtcgagccg ccacccggat
     8581 tcgatcgggc acggctacgg cgtcacctcg atcgtcgact gcgcctcctg ccacacgcgc
     8641 gacaacagtc ccgattacga ccccgggctg tacgtcccgc aggtgcgcca ctggggtgag
     8701 gcgcaggccg ggcactagct cc
//
LOCUS       DASRIQ010000081         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_31741, whole genome shotgun sequence.
ACCESSION   DASRIQ010000081 DASRIQ010000000
VERSION     DASRIQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31741"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..989
                     /locus_tag="VFD06_02050"
     CDS             156..989
                     /locus_tag="VFD06_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02346.1"
                     /translation="MRISTGPGATLVACVLALAGCRATPPAEPESLLETWIRRHTEAR
                     GGAAAIERVRALRYRLTITEPSFTVEGVYEVDREGRMRIDILKDGRRVFTEGFDGRSG
                     WQQREGEEHGREASPEGSAALWHGTQSPDKLFGLHEMTRLGHQVVAAGEEDVEGKKYA
                     RVRVTLSDGWETMYYLDPDTWLVARSRDHKALHVDVDPTKRPLEESYSDTRAVAGVKR
                     SFRAVGRDLASGEVIQTTTVTGIEVNPPLDSTRFNRPRRGAGGRRRSSGSRRGSSRRR
                     R"
     gene            complement(910..1968)
                     /locus_tag="VFD06_02055"
     CDS             complement(910..1968)
                     /locus_tag="VFD06_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZN02347.1"
                     /translation="MRRALRPALLVVLAAGIAAHVLETRRIGRPLAEPGAGGAAGGPG
                     PGAAGAVAPRLDAAALLEDLETLASPSFEGRRTDSDGGRRARAFVVERFERLGLEAAV
                     PGYRQPFSFTHRSIRALWRRDRPFVLEFKDAANVLGLVRGTVDADRFLIVSAHYDHLG
                     IRKDDLYPGADDNASGVAALLGIAAYFTAHPPRHTLLFAAFDAEEQGCRGSEAFAQHP
                     PIPLDRLILDVNMDMIGRSEPGRLFVAGTHQDPRFLPLLREAAGRSAVALHAGHDRPI
                     WRTGLVDDWTTGSDHRPFAERGLPFLYFGVEDHEDYHAPGDTFERIDRDFFTGAAETI
                     LDVILTIDAAPPLLAAAG"
     gene            complement(2018..>3253)
                     /locus_tag="VFD06_02060"
     CDS             complement(2018..>3253)
                     /locus_tag="VFD06_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HZN02348.1"
                     /translation="AEGGPDGTYNEILALNGIVSDVSSQVKWIDEWGGPKTFPHFAVG
                     WAHAGNTPFQWTKQVASHFGGTRNPLIVHWPKRIPGHGEVRLQFSHVIDIAPTALEAA
                     AIPQPKSVDGAQQYAMDGVSLMYAIQDPKAAERHTTQYFEMFGNRAIYHDGWVAATRH
                     SIPWMMTQNPPLDQDRWELYNVAEDFSQATDLAASNPEKLKELQDLFTKEAIRNHVFP
                     IDDRRAERFDAAIAGRPDLLGPRTSLTLYEGMTGIAENAFLNLKSRSHSITADVNVPA
                     GANGVIISQAGRFGGWTLYMKAGKPRYTYNYGGLEWTTIEAPQPLAAGRHTLRFEFAY
                     DGGPPGTGGTGRLAVDGKPAGEKKIVRMMPFVYSADEGVDVGMDQETPVSEDYKAGDN
                     AFTGRVLKVTVEAIPPAAK"
BASE COUNT          489 a         1086 c         1190 g          488 t
ORIGIN      
        1 cggctacaac aaggtggggc cgcctccggg tggcgcaccg cctcccggca gcggactgca
       61 ccctcctcgg aacacataaa cctttcatcg ggattggggc gggcacggaa gtcacgaggc
      121 aaccccacct cgttggtgcg cgtatccgac gcgcgatgcg gatttcaacc ggaccgggcg
      181 cgaccctcgt ggcgtgcgtc ctggcgcttg cggggtgccg tgcgacaccg ccggcggagc
      241 cggagtcgct cctcgagacg tggatccggc ggcacaccga agcgcgcggc ggggcggccg
      301 ccatcgagcg cgtgcgagcg ctgcgctacc gcctgacgat caccgagccg tcgttcaccg
      361 tcgaaggggt gtacgaggtg gaccgggagg gccggatgcg catcgacatt ctcaaggacg
      421 ggcggcgcgt cttcaccgag gggttcgacg gacggtccgg ctggcagcag agggaggggg
      481 aggagcacgg acgtgaagca agtccggagg ggagcgcggc cctctggcac ggcacccaga
      541 gccccgacaa gctgttcggg ctgcacgaga tgacgcggct ggggcaccaa gtcgtggcgg
      601 ccggcgagga ggacgtggaa ggaaagaagt acgcgcgggt gcgcgtcacc ttgagcgacg
      661 gctgggagac gatgtactac ctcgatccgg acacctggct cgtggccagg agccgcgatc
      721 acaaggcgct ccacgtcgac gtcgacccga cgaagcggcc gctcgaggag tcctactccg
      781 acacgcgcgc ggttgcggga gtgaagcgca gcttccgtgc cgtcgggcgc gacctcgcgt
      841 cgggcgaggt gatccagacg accaccgtca ccggcatcga ggtcaacccg ccgctggact
      901 ccacccggtt caaccggccg cggcgaggag cgggggggcg gcgtcgatcg tcaggatcac
      961 gtcgaggatc gtctcggcgg cgccggtgaa gaagtcccgg tcgatccgct cgaaggtgtc
     1021 gcccggggcg tgatagtcct cgtggtcctc cacaccgaaa tacaggaagg ggagcccgcg
     1081 ctcggcgaag ggccggtgat cggatccggt cgtccagtcg tccaccaggc cggtgcgcca
     1141 gatcggccgg tcgtggccgg cgtgcagcgc gacggcgctg cgtcccgccg cctcgcgcag
     1201 gagcggcagg aagcgcgggt cctggtgtgt gccggcgacg aacagccggc cgggctcgct
     1261 gcggccgatc atgtccatgt tcacgtcgag gatcagtcga tcgagcggga tcgggggatg
     1321 ctgcgcgaac gcctcggagc cccggcagcc ctgctcctcg gcgtcgaacg cggcgaagag
     1381 gagcgtgtgc cgcggcgggt gggccgtgaa gtacgccgcg atgccgagga gcgccgcgac
     1441 gccgctggcg ttgtcgtcgg cgcccggata caggtcgtcc ttgcggatcc cgagatgatc
     1501 gtaatgggcg gagacgatga ggaagcggtc cgcatccacg gtgccgcgca cgagtcccag
     1561 gacattcgcg gcgtccttga actcgaggac gaacggccga tcgcgccgcc acagcgcccg
     1621 gatgctgcga tgggtgaagg agaagggctg ccgatatccg gggaccgccg cttcgaggcc
     1681 gagccgctcg aagcgttcca cgacgaaggc gcgggcgcgg cggccaccat cgctgtcggt
     1741 gcggcgccct tcgaacgacg gggaggcgag ggtctcgagg tcctcgagga gcgccgccgc
     1801 atcgagacgc ggcgccaccg cgccggccgc gccgggaccc ggaccgcccg cggcgccccc
     1861 tgcgcccggc tccgccagcg ggcggccgat gcgccgcgtc tcgaggacgt gcgccgcgat
     1921 gccggctgcg agcacgacga gcagcgccgg gcgcagcgcc cggcgcactc gcgccgcgtg
     1981 cgaccgccgg tcgggggacg atcgatcgac cagaggacta cttcgccgcg ggggggatcg
     2041 cctccaccgt caccttgagc acgcgcccgg tgaaggcgtt gtcccccgcc ttgtagtctt
     2101 ccgagactgg agtttcctgg tccatgccga cgtccacccc ttcatcggcg gaatagacga
     2161 acggcatcat ccggacgatt tttttctctc ccgccggctt cccgtccacc gccaggcgac
     2221 cggtgccgcc ggtccccggt ggtccgccgt cgtacgcgaa ctcgaagcgc agggtgtgcc
     2281 ggccggcggc gagcggctgc ggcgcctcga tcgtcgtcca ctccaggccg ccgtagttgt
     2341 aggtgtagcg gggtttgccg gccttcatgt agagggtcca accgccgaag cgacccgcct
     2401 ggctgatgat gacgccgttg gcgccggccg ggacgttgac gtccgcggtg atgctgtggg
     2461 accgcgactt gagattgagg aaggcgttct cggcgatccc ggtcatccct tcgtacagcg
     2521 tcagggacgt cctgggtccc aggaggtcgg gccggccggc gatcgccgcg tcgaaccgct
     2581 cggcgcgccg atcgtcgatc gggaagacgt ggttgcggat cgcctccttc gtgaacagat
     2641 cctggagctc tttcagcttc tccgggttcg aggcggcgag gtcggtcgcc tggctgaaat
     2701 cctccgccac gttgtacagc tcccacctgt cctgatcgag cggcgggttc tgggtcatca
     2761 tccacgggat ggagtggcgc gtcgccgcca cccagccgtc gtggtagatc gcccggttgc
     2821 cgaacatctc gaagtactgg gtggtgtgcc gctcggcggc cttcggatcc tggatcgcgt
     2881 acatcagcga gacgccgtcc atcgcatact gctgcgcccc gtccacgctc ttcggctgcg
     2941 ggatggcagc cgcctcgagg gcggtcgggg cgatgtcgat cacgtggctg aactgcaacc
     3001 gcacctcgcc atgccccggg atcctcttcg gccagtggac gatgagcgga ttgcgggtgc
     3061 cgccgaagtg cgaggcgacc tgcttggtcc actggaacgg cgtgttgccg gcatgcgccc
     3121 agcccaccgc gaagtgaggg aaggtcttcg gaccgcccca ctcgtcgatc cacttcactt
     3181 gggaggagac atcgctgacg atgccgttca gggccaggat ctcgttgtag gtgccgtccg
     3241 gccccccctc ggc
//
LOCUS       DASRIQ010000082         5135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2643630, whole genome shotgun sequence.
ACCESSION   DASRIQ010000082 DASRIQ010000000
VERSION     DASRIQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643630"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFD06_02065"
     CDS             <1..717
                     /locus_tag="VFD06_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN02349.1"
                     /translation="SAAELERAQASLAGLEARLRSARQGAGGGREGGATAGDGFAVAA
                     PWAGRVAAVSVTPGQTVTAGAPLGRLVKLRPLWIVLALRPEDAAKVREAPSGLLLRRP
                     GSAETLRLDGKEVRVVSRSPEIDPRTASIDLIVEIDRSAADLAIGSGVEADLLLGGAK
                     RGIVIPTSALVDDADVLVAYVQLEGESFARREVKVLARQGDRALVEGLRPGERLVITG
                     GGAIRRAALLSSGAPEGHVH"
     gene            725..3808
                     /locus_tag="VFD06_02070"
     CDS             725..3808
                     /locus_tag="VFD06_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN02350.1"
                     /translation="MLNAIIRGSLRHRWAVLTIAAASVVAGSWRAWRMPVDVFPDLSA
                     PTVTVVTEATGLAPEEVELLVTFPIESALNGASGVRRIRSVSGPGIGVIWVEFEWNED
                     VYRARQVVSERLQVLTLPAGVDRPMLGPISSIMGEITFVALTSDTVPPMELRRLAETG
                     VRRSLLALPGISQVVPIGGDVREYLVELDPAAGTQAGLSVREVVDALEEASAAPAAGF
                     HVDAGEEYLVRGLGRARSSADLAATVLRQHAGIPLRLGQVASVREAPEPPRGTASYRA
                     HPAVILSVQKQPGANTLEVTGRVDRVLDDLQKTLPEGVVIEKENFRQADFISVALRNV
                     SVALRDGAVLVLVVLFLFLGSVRTTIISAVAIPLSLVAGVLTISAFGGTLNTMTLGGF
                     TIAIGALVDDAIIDVENVLRRLRENRRRPESDRRPALEVVFRASSEVRGAILFATLVI
                     ALVLVPLLNLPGLEGRLLRPLGVAYLASLAGSILVALTVTPVLCLFLLPRGRLLDKEE
                     PWLLRRCKQAYDRTLAVCLDRTGLIVGGAAVALAASLAVIPFLGHSFLPPFNEGSLTI
                     SVVSPPGTPLVEGDVLGRQVEEALLAFPETVSTSRRSGRAERDEHIQGVNASELEVVL
                     RPGRPKAELLAEMRRAVATIPGVSASFGQPISHRIDHMISGSRTNLAVKIFGPDLAVL
                     RGLSASAERLLHDVPGIVDLSNQEQASVPQLLIDFDRGAMARQGLSSADLAQSVEALF
                     QGTPAGEIVEGGLTSRVMVRYPERLRARRDQMDALPVTTPDGRRIRLGDVAQVRFDLG
                     PGLIRRESVERVAMVTANVTGTDLTGVVERARRALARGLPLPEGYRIAFGGQFEEAAR
                     SLQNLALLGAGLLVAMHGLLYLAFRSHRQALIVLANLPLALIGGIAAVALGGRGLSVA
                     SLVGFITLFGIATRNGVLLVSHYGHLIREEGVTLRDAVVRGSHERLAPVLMTALTAGL
                     ALIPLVLAGDEPGNEIQSPMAEVILGGLLSSTFLNLVVVPVLFRRWGQPRAPSAT"
     gene            complement(3846..4517)
                     /locus_tag="VFD06_02075"
     CDS             complement(3846..4517)
                     /locus_tag="VFD06_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02351.1"
                     /translation="MFVGHLAVALAAKKRTPRVSLGVLMAATAFIDLIWPPLLLLGLE
                     RARIDPGNTAFTPLAFDSYPWSHSLAMVLVWGALVGLAAGRYYKDRASGVVVGGVVVS
                     HWFLDAISHRPDMPLWPGGPVVGLGLWNSIPLTLVVEGAMWAAGLAIYLRATRPADRA
                     GQVIFWSLVVLCTVMWATGPWSPPPPSVEALSKFAFGAWLLPLWAGWADAHRRPRSAA
                     VAPAA"
     gene            complement(4649..5026)
                     /gene="crcB"
                     /locus_tag="VFD06_02080"
     CDS             complement(4649..5026)
                     /gene="crcB"
                     /locus_tag="VFD06_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HZN02352.1"
                     /translation="MTLVWVGIGGCLGSVLRYLLSEYVQGRAGGPFPLGTLAVNILGC
                     FAIGGLTGLFEGTMLPPPEARAFLVAGILGGFTTFSAFGNETLLLAREAGRGPALANV
                     AAQVLLGLGAVWLGRTLVQAIWK"
BASE COUNT          714 a         1811 c         1862 g          748 t
ORIGIN      
        1 agcgccgccg agctggaacg ggcgcaggcc agcctcgccg gtctggaagc gcgcctgcgg
       61 tcggcgcggc agggggcggg cggggggagg gagggcggag cgacggccgg ggacggtttc
      121 gccgtcgccg caccctgggc cggccgggtg gccgcggtct ccgtcacacc cggccagacc
      181 gtgacggccg gcgccccgct cgggcggctg gtgaagttgc ggccgctctg gatcgtcctg
      241 gcgcttcggc ccgaagatgc cgccaaggtc cgcgaagcgc cttccgggct gctcctccgg
      301 cggcccggat ccgccgagac cctcaggctc gacgggaagg aggttcgcgt cgtctcccgc
      361 tcgccggaga tcgacccgcg caccgcctcc atcgacctga tcgtcgagat cgatcgcagt
      421 gccgccgatc tggccatcgg gagcggtgtc gaggcggatc tgctgctcgg cggtgcgaag
      481 cgcgggatcg tcatcccgac ctcggcgctg gtggacgacg ccgacgtcct ggtcgcctac
      541 gtgcagctcg aaggggagag tttcgcccgg cgcgaggtga aggtgctggc gcgtcagggc
      601 gatcgggcgc tcgtcgaggg cctgcgcccc ggcgagcggc tggtgatcac cggcggcggc
      661 gccatccggc gcgccgcgct tctctcctcg ggggctcccg aggggcacgt ccactgagac
      721 gccgatgctg aacgccatca tccgtggttc gctgcgccat cgctgggcgg tgctgacgat
      781 cgccgccgcg tccgtggtcg cgggatcgtg gcgcgcctgg cggatgccgg tggatgtgtt
      841 ccccgatctg tcggctccga ccgtcaccgt cgtcaccgag gcgaccgggc tcgcgccgga
      901 agaggtcgag ctgctcgtca ccttcccgat cgagtcggcg ctgaacggcg cttccggcgt
      961 gcgccgcatc cgctccgtct ccggtcccgg gatcggcgtc atctgggtgg agttcgaatg
     1021 gaacgaggac gtctaccgcg cccggcaggt ggtcagcgag cggctccagg tgctgactct
     1081 cccggccggg gtggaccggc cgatgctcgg gccgatctcc tcgatcatgg gggagatcac
     1141 cttcgtggcg ctgacgtccg atacggtgcc gccgatggag ctgcggcggc tcgcggagac
     1201 cggggtacgg cgcagcctgc tggcgctgcc cgggatttcg caagtggtcc cgatcggcgg
     1261 ggacgtccgc gaatacctcg tcgagctgga cccggccgcg ggaacgcagg ccggcctgtc
     1321 ggtcagggag gtcgtggacg cgctcgaaga ggcgagcgcc gccccggccg ccggattcca
     1381 cgtcgacgcg ggggaggagt acctcgtccg cggcctcggc cgcgcccgct cgtccgccga
     1441 cctcgcagcc accgtcctgc ggcagcacgc cggcattccg ctgcgcctgg gacaggtggc
     1501 gagcgtgcgc gaagcccccg agccgccgcg agggaccgcg tcgtaccgcg cccatccggc
     1561 ggtcatcctg agcgtccaga agcagcccgg agccaacacg ctcgaggtca ccggtcgcgt
     1621 ggatcgcgtc ctcgacgacc tgcagaagac ccttcccgag ggcgtggtga tcgagaagga
     1681 gaacttccgg caggccgact tcatctccgt cgccctccgc aacgtctcgg tggcgctgcg
     1741 cgatggggcg gtgctggtgc tcgtcgtcct gttcctgttc ctcggaagcg tgcgcacgac
     1801 gatcatctcc gccgtcgcca tcccgctctc gctggtcgcg ggcgtcctga cgatctccgc
     1861 gttcggcggc acgctgaaca cgatgacgct cggcgggttc accatcgcca tcggggcgct
     1921 ggtggacgac gccatcatcg acgtcgagaa tgtcctgcgc cggctgcgtg agaatcgccg
     1981 ccgtcccgag agcgatcgcc ggccggcgct cgaagtggtc ttccgcgcct cctccgaggt
     2041 gcggggcgcc atcctgttcg cgacgctcgt gatcgcgctg gtgctcgtcc cgctcctgaa
     2101 cctgccgggg ctggaggggc gcctgctgcg tccgctgggg gtggcctatc tcgcgtcgct
     2161 cgccgggtcg atcctggtcg ccctgaccgt gacgccggtc ctgtgtctct tcctgctgcc
     2221 gcgcggcagg ctgctcgaca aggaggagcc gtggctcctg cgccgctgca agcaggctta
     2281 cgatcggacg ctggcggtgt gtctcgatcg gaccggcctg atcgtcggcg gggcggcggt
     2341 cgcgctcgcc gcgtcgctgg cggtgatccc tttcctgggg cattccttcc tgcctccgtt
     2401 caacgaagga agcctgacga tctccgtcgt cagccctccg ggaacgcctc tcgtggaagg
     2461 ggacgtcctc ggccgccagg tggaggaggc gctcctggcc ttccctgaga cggtttcgac
     2521 cagccggcgc agcgggcggg ccgagcggga cgaacacatc cagggggtga acgcctcgga
     2581 gctggaagtc gtgctgcggc cggggcggcc gaaggcggag ctcctggcgg agatgcgccg
     2641 ggcggtcgcc accatccccg gcgtcagcgc cagcttcgga cagccgatca gccaccgcat
     2701 cgatcacatg atctccggca gcaggacgaa cctcgccgtc aagatcttcg gcccggacct
     2761 cgcggtgctg cgcgggctgt cggcgagcgc cgagcgcctg ctgcacgacg ttcccggcat
     2821 cgtcgatctc tccaaccagg agcaggcgtc ggtcccgcag ctcctcatcg acttcgaccg
     2881 cggggcgatg gcgcgccagg gactgagttc cgccgacctg gcgcagtcgg tcgaggcgct
     2941 gttccaggga accccggcgg gggagatcgt cgaaggcggg ctcacctccc gcgtcatggt
     3001 gcgttacccg gagcggctgc gcgcgcgacg cgatcagatg gacgcgctgc cggtgacgac
     3061 tcccgacggc cggcgcatcc ggctgggcga tgtggcgcag gtgcggttcg atctggggcc
     3121 cggtctcatc cgacgcgaga gcgtcgagcg ggtggccatg gtgacggcca atgtgaccgg
     3181 gaccgacctg accggtgtcg tcgagcgggc gcgtcgcgcc ctcgcccgcg gtctgccgct
     3241 gcccgagggg tatcggatcg cgttcggcgg gcagttcgag gaggcggccc gcagcctgca
     3301 gaacctcgcc ctgctcggcg ccggcctcct cgtggcgatg cacgggctcc tgtatctcgc
     3361 gtttcgcagc caccggcagg cgctcatcgt gctcgcgaac ctgccgctgg cgctcatcgg
     3421 cgggatcgcc gccgtcgcgc tcggagggcg cggcctgagc gtcgcgtcgc tcgtcgggtt
     3481 catcacactc ttcgggatcg ccacccgcaa cggcgtgctc ctggtgagcc actacgggca
     3541 cctgattcgt gaggaagggg tgacgctccg cgacgccgtc gtgcgcggat cgcacgagcg
     3601 cctcgcgccg gtgctgatga cggcgctgac cgccgggctg gcgctcatcc ccctggtcct
     3661 cgcgggagac gagcccggca acgagatcca gagcccgatg gcggaagtga tcctcggagg
     3721 gctgctctcc tcgaccttcc tgaacctggt ggtggtcccg gtcctgttcc gccgctgggg
     3781 ccagccgcgc gcgccttcgg cgacctgacc ctgccgaccc cacgaccgcg actagcggcg
     3841 gggcctcacg ccgctggcgc gaccgcggcg gagcgcggcc ggcggtgcgc atcggcccag
     3901 ccggcccaga gtggaagcag ccaggcgccg aaggcgaact tcgagagcgc ctcgacgctg
     3961 ggcggtggcg gagaccacgg gccggtcgcc cacatcaccg tgcacaggac cacgagcgac
     4021 cagaagatga cctggccggc tcgatcggcc ggccgcgtcg cgcgcagata gatcgccagc
     4081 cccgcggccc acatcgcccc ctcgaccacg agcgtcagcg ggatcgagtt ccagagaccg
     4141 agaccgacga ccgggccgcc cggccagagc ggcatgtccg gccggtgcga aatcgcgtcg
     4201 aggaaccagt ggctcacgac cacgccgccg accacgacgc cggaggcgcg atccttgtag
     4261 tacctgccgg ccgcgagccc cacgagcgcg ccccacacga gcaccatcgc gaggctgtga
     4321 gaccatgggt acgagtcgaa cgccagcggc gtgaaggcgg tgttgccggg gtcgatccgg
     4381 gcccgctcga gtccgagcag cagcagcggc ggccagatca gatcgatgaa tgcggtcgcg
     4441 gccatgagaa cgccgagcga gacgcgcggc gtccgcttct tcgccgcgag cgcgaccgcc
     4501 agatggccga cgaacatcgc cggccgagca tactcctacc tagctcttcg ccgtcccagc
     4561 gatagagctg ttgcaacgtg gacggatttt gagggcatcg cgatctcccg cggggggggc
     4621 tgagcgtacg gcgatcaccg ggatcgcgct acttccagat cgcctgcacg agcgtccggc
     4681 cgagccagac ggcgccgagc ccgaggagga cctgggcggc gacattcgcg agagcgggac
     4741 cgcggccggc ttcgcgcgcc agaagaaggg tctcgtttcc gaaggcggag aaggtggtga
     4801 accctccgag gatccccgcg acgaggaacg cgcgcgcctc gggaggcggc agcatcgttc
     4861 cctcgaagag gccggtgagc ccgccgatcg cgaagcaccc gaggatgttg accgcgagcg
     4921 tgccgagcgg gaagggaccg cctgcgcgtc cctgcacgta ttcgctgagc aggtagcgca
     4981 ggacggatcc gaggcagccg ccaatcccca cccacacgag ggtcacctgg acctcacagg
     5041 aatccgcacg gccgcatcgt agtccggacc cacaaaaaaa gggcgggagc ccgaaggctc
     5101 ccgcccccca ccctaccgtt cagagcgaat cagcg
//
LOCUS       DASRIQ010000083         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_286625, whole genome shotgun sequence.
ACCESSION   DASRIQ010000083 DASRIQ010000000
VERSION     DASRIQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286625"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VFD06_02085"
     CDS             complement(<1..1261)
                     /locus_tag="VFD06_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZN02353.1"
                     /translation="MPPRSHPSRPMPWVPLPLVVLAAGLLATALLPGTDRLDAAPTQV
                     TARLRVMNLNIFYGGDELNLQNGNFCVRADGCPETLALVVEEIRRADPDVVGIEEGEH
                     NACAIAQALGWNCSARMQIISRLPLIDPPGGDGVYLFVQIAPGKVVAMMNVHLPSDPY
                     GPYVVRDGGTADEVHALETSVRLPAIQEQLAALPGLLRAGIPVFLTGDFNSPSHHDWT
                     TEVAAVRPEVRFPFDWPVSRALADAGFFDPYRVVHPDPIAVPGFTWSPGGPETVTNDV
                     WDRIDWVLAAGNAVPIASTVVGEVGFPDAGVTIDPYPTDHRGVLSTFDVTPGTSPILV
                     AVGSRRVFAGETLMVTFHARGRNGEAVAIVPAGGTAGAAIVRRPTGGATDGTFPFGTS
                     SLAPGAYEAALLDSAGSIVSRSPFWLYA"
     gene            complement(1391..2371)
                     /locus_tag="VFD06_02090"
     CDS             complement(1391..2371)
                     /locus_tag="VFD06_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02354.1"
                     /translation="MSAGVAVARRRAGVGPGALWVLAAILVPAMAAPWIAPRPPDLQE
                     DVAGARDLPPLTRAHALIGADGRTRIVTGLRRAGTVWEGFRSGAIARVDEADLASPPA
                     ARIFLLGTDRLGRDVLSRVLFGLRHSAGVAALAVALSLLLALLAGSLAVFGGTLADAV
                     IMRGIDVLMAVPRLLVYLLCAALLVPSTPLLVGVFAVTTWTGLARIVRVELMALRGGG
                     LEAAARAAGARPLRTLFRHLLPAILPALAVATALRFADTILLESAVAVLGLGAPPPAV
                     SLGDIMASGRSAAMDWWLVAVPGALLAGLVLALRSAATSLDRLLDPPSIT"
     gene            complement(2368..3315)
                     /locus_tag="VFD06_02095"
     CDS             complement(2368..3315)
                     /locus_tag="VFD06_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02355.1"
                     /translation="MSAFLLRRFLFALPILLGALTLLFLLMEALPGRPFPSEPGALAP
                     EAAVRLRRLYGTDRPVAERYLLWLGGAARGDLGFSFSEREPVAATVGRAAGRTFVLAG
                     IAIFLQFVAGTAAGMLAAAGGRRVDRAVTGAAALLYAVPSYWLGLLLVAIFSVRLGWL
                     PASQMRSAGGAGLADAVRHLLLPCLALALPAAGGIALFVRDAIRAGLGERLTAAVRSR
                     GLSRAQIVVRHGAWRGLLAASTLLGLALPGLVAGSVVIETLFAWPGMGRLAYQATLAR
                     DAPLVLGCAACAAALVIAGSLLADLLAAAIDPRVRESLE"
BASE COUNT          559 a         1262 c         1316 g          339 t
ORIGIN      
        1 gggcgtacag ccagaagggg ctgcgcgaca cgatggaccc ggcggagtcg agcagcgcgg
       61 cctcgtacgc gcccggcgcc agggacgacg tcccgaacgg gaaggtgcca tcggtcgcac
      121 caccggtcgg ccggcggacg atcgcggcgc cggccgtgcc accggccggc acgatcgcca
      181 ccgcctcgcc gttgcggccg cgcgcgtgga aggtgaccat gagggtctcc ccggcgaaca
      241 cccggcgcga gccgaccgcc accaggatcg gcgacgtccc cggcgtcacg tcgaaggtcg
      301 agagcacccc gcggtgatcg gtcgggtacg ggtcgatcgt gacgccggcg tcggggaagc
      361 cgacctctcc gacgaccgtg ctcgcgatcg ggacggcgtt ccccgccgcc agcacccagt
      421 caatccggtc ccagacgtcg ttcgtcaccg tctccggacc ccccggcgac caggtgaacc
      481 cggggacggc gattgggtcc ggatgcacga cgcggtacgg atcgaagaat ccggcatcgg
      541 cgagggcgcg gctcaccggc cagtcgaacg ggaagcgcac ctcggggcgg accgcagcga
      601 cttccgtggt ccagtcgtgg tgcgacggcg agttgaaatc accggtcaga aagaccggga
      661 tcccggcgcg cagcagcccc gggagggcgg cgagctgttc ctggatcgcc ggcaggcgca
      721 cggaggtttc gagagcgtgc acctcgtccg ctgtgccgcc gtcgcgcacg acgtacggcc
      781 cgtacggatc ggacggcagg tgcacgttca tcatcgccac gaccttgccg ggggcgatct
      841 gcacgaacag gtagacgccg tcccctcccg gcggatcgat gagcggcagc cgggagatga
      901 tctgcatccg ggccgagcag ttccacccga gcgcctgcgc gatggcgcag gcgttgtgct
      961 ccccctcctc gatcccgacg acgtccgggt cggcccgccg gatctcctcc accaccagtg
     1021 ccagcgtctc gggacagccg tcggcgcgga cgcagaagtt gccgttctgg aggttgagct
     1081 cgtccccgcc atagaagatg ttgaggttca tgacgcggag gcgcgccgtc acctgtgtgg
     1141 gggcggcatc gagacggtcc gtccccggga gcagcgccgt ggcgagcaga ccggcggcga
     1201 gcacgacgag cgggagcgga acccacggca tcggacggga cgggtggctt cgcggcggca
     1261 tcgggacccc ctcggggcga aaggccccgg cggatgcgga ttgttcctgc gtcgggcaga
     1321 tgccgcgatt ctagcccgga tccgggtcga gcagccccat ctccgccgtg cgggccggct
     1381 gcgtcgaagg tcaggtgatc gacggagggt cgagcagccg gtcgagcgac gtggccgccg
     1441 atcgcagcgc gaggacgagc cccgccagga gcgcgcccgg cacggcgacg agccaccagt
     1501 ccatggcggc cgaccgtccc gacgccatga tgtcgccgag ggagaccgcg gggggcgggg
     1561 cgccgagccc gagcaccgcg accgccgact ccagcaggat cgtgtcggcg aagcgcagcg
     1621 cggtggcgac ggcgagcgcc gggaggatcg cggggagcag gtggcggaag agggtgcgga
     1681 gcgggcgcgc cccggcggcg cgggccgccg cctcgagacc gccgccgcgc agcgccatca
     1741 attcgacccg gacgatgcgc gccagcccgg tccaggtggt gaccgcaaag acgccgacga
     1801 gcagcggcgt cgacgggacg agcagggcgg cgcagaggag gtacacgagg aggcgcggca
     1861 ccgccatgag gacgtcgatc ccccgcatga tcacagcgtc cgcgagcgtc cctccgaaga
     1921 ccgccagcga gccggcgaga agcgcgagca gcagcgacag cgcgaccgcg agcgccgcga
     1981 cccccgcgga atggcgcaga ccgaacagga ctcggctcag cacgtcgcgc cccaggcggt
     2041 cggtccccag aaggaagata cgcgcagcgg gaggggaggc gagatccgct tcgtccaccc
     2101 gcgcgatcgc gccggaccga aatccctccc agacggtgcc ggcacggcgc aggccggtga
     2161 cgatccgggt gcgcccgtcg gcgccgatga gcgcgtgcgc ccgcgtgagc ggcgggaggt
     2221 cgcgcgcccc ggcgacatct tcctgaaggt ccggagggcg cggggcgatc cacggcgccg
     2281 ccatggccgg caccaggatc gcggcgagga cccagagggc ccccggtccc accccggccc
     2341 gccgccgggc gacagcgacg ccggcgctca ttcgagcgac tcccgcacgc gcgggtcaat
     2401 cgccgccgcc agcaggtcgg ccagcaggct cccggcgatc accagcgccg cggcgcaggc
     2461 ggcgcagccg aggacgagcg gcgcgtcgcg cgccagcgtc gcctggtagg cgaggcgccc
     2521 catgccgggc caggcgaaca gcgtctcgat cacgacgctg ccggcgacga gccccggaag
     2581 cgccagcccg aggagggtcg aggcggcgag gagtccccgc caggctccgt ggcgcacgac
     2641 gatctgggcg cggctcaggc cgcgcgaacg caccgcggcg gtcagccgtt caccgagccc
     2701 ggcgcggatg gcgtcgcgca cgaacagggc gatgccgccg gcggccggga gcgcgagggc
     2761 gaggcaaggc agcaacagat gccgcacggc gtccgcgagg ccggcaccgc ccgccgagcg
     2821 catctgcgac gccggcagcc accccaggcg gaccgagaag atcgcgacca gcagcaggcc
     2881 gagccagtag gacgggaccg cgtacaggag cgccgcggct ccggtcacgg cccgatcgac
     2941 ccgccggccg cccgcggcgg ccagcatccc cgccgccgtc ccggccacga actgcagaaa
     3001 gatggcgatc cccgcgagga cgaacgtccg gcccgccgcg cgtccgaccg tcgccgcgac
     3061 cggctcccgc tccgagaagg agaagccgag atcgccgcgc gccgcccccc cgagccacag
     3121 gagatagcgc tccgccacgg ggcggtccgt gccgtagagc cggcgcaggc gcacggccgc
     3181 ctccggcgcg agcgctccgg gctcggaggg gaagggccgt cccggcagcg cctccatgag
     3241 caggaacagc agtgtcagcg ccccgaggag gatcggcagg gcgaagagga aacgccggag
     3301 gaggaaggcg ctcacgcctg gggccgcgcg atcgatcctt gtggcccgcg ggggccgagc
     3361 gtaggatccc cacagaccat aggtcgcctt tccggatgag acgagccgat ccgatccttc
     3421 cgcccgggcc caagcgcccc ggacggcgca gcgggtttca ctccgccgcg atcgcg
//
LOCUS       DASRIQ010000084        13835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3425719, whole genome shotgun sequence.
ACCESSION   DASRIQ010000084 DASRIQ010000000
VERSION     DASRIQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3425719"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFD06_02100"
     CDS             complement(<1..569)
                     /locus_tag="VFD06_02100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN02356.1"
                     /translation="MALAPGTRLGPYEVIAPLGAGGMGEVYRARDARLGRDVAVKVLP
                     AAFAVHADRLARFEQEARATGALNHPNILAIHDIGSHEGMPYLVSELLEGETLRERVA
                     QGPLPVRKATDLAAQAARGLAAAHERGIVHRDLKPENLFITRDGRIKILDFGLAKLLQ
                     PDVQAGPVTSLPTTPASTEAGVIMGTVGY"
     gene            complement(614..994)
                     /locus_tag="VFD06_02105"
     CDS             complement(614..994)
                     /locus_tag="VFD06_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011769909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HZN02357.1"
                     /translation="MIHFELLRDRGILILEPRGALTAADFTALAGPVDPYLEEHGDLK
                     GLVVDAPSFPGWDDFAALVSHLRFVRDHHKRIRRIAVVSDSALLSAAPKIASHFVNAE
                     IRNFKAGERAAALAWLESPPGPAA"
     gene            complement(1116..1997)
                     /locus_tag="VFD06_02110"
     CDS             complement(1116..1997)
                     /locus_tag="VFD06_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN02358.1"
                     /translation="MSSLGLYAISVLIWGSTWLAITFQFGTVPPAVSVVYRFLLAGAI
                     LFAWSALKRIRLRFSAREHLWMALQGVLLFGINYLCVYLAETHITSGLVAVAFSLLTV
                     LNIVGSRLFFGTPLKPAVMLGAALGIAGVALVFLPEIRGGTARGSAGLGIALALAGAV
                     SASLGNLVAYRNHARGLPVVPMNTFGMVYGGLCIGAYALATRQPWRFDLSARYLVSLG
                     YLAIFGSIVAFGAFLVLLGRIGADRAGYITVAIPVVALLLSGLFEGLRWHPSLAAGIL
                     LCIAGNVAVLRSRRPAH"
     gene            complement(1994..3166)
                     /locus_tag="VFD06_02115"
     CDS             complement(1994..3166)
                     /locus_tag="VFD06_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZN02359.1"
                     /translation="MAIACLGAALAGSPVRAAGDADAEDAAEGSSTTATPKPKLRSEE
                     DGEFDVSSFLDKAYGFVPILVPITEPAVGYGGALGCVFINRPIAESAKAGFGRPNLSF
                     VGGAATENGTRAVMAGDVRQWMDDRVQTVAAILDAKVNLDFYGIGKDETLEHDPLHYT
                     LEPLGGGVRGRYRLGASRLWVGMSYALADTHISFDDESLAAALLDPPEDSRIGGLTPS
                     IAFDSRDNIFTPTRGTYFEGSTGVFDESLGGDTSFQRVALTWIVYRPLHQRVFFGVRS
                     GLTVSFSDPPFYMLPFITLRGAPVMRYQGEEAAEIETEIRWQCWKRWSVVGFVGTGAA
                     WNDLEETEDVKSIVTGGTGVRYELARKYGLHVGADVAWGPDDSALYIQFGSAWARP"
     gene            3406..5454
                     /locus_tag="VFD06_02120"
     CDS             3406..5454
                     /locus_tag="VFD06_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02360.1"
                     /translation="MRSRLRTLGGASAVLAVLLALQPGAGIAAPAAAPAAPAASAAAA
                     PVLSPAWSARLDGMVEWQRVAPLGQLLVKTTLGLTGFDPERGRAIWKQPGLGGMAQDH
                     YEEIDGTSLVVLSDGLAKPRVVILDSVDGRILFDSRAYGVAQVLSRHYLPKSRALLLF
                     GFSEGDPATTMYLADVDRGTIRWKNNRLLEGQGKFTRALTSFLQAATNQSGIISDPLE
                     IASDTFVIASTTEILAVRTATGEIAWRVPNVHNTRSTRFHADEGRAPGLLFVGSETAF
                     TSMTSTAGAQKSETIYSEYSARRMADGAAAWGKPVRVKGGLNDVIFADRGLVVSPRTT
                     GKGKILLCDYRSGEPLWGKKGKGIDITGGIINHDWTRAGLVLTTGYDSAWSNKGTEYF
                     LTLVDPATGLPRFEEPLRLRGRVVSTQVLPAGLLYTTTSEVNILDLKTGQPLLGDGVR
                     SDESLVTTTAGSSLFAYAGKHGTLHRLDLDRAALSTVSSAPERLEEDEAPMAIEADGN
                     RITVLSSQNVIAWNADGSLLFHAYHPSPRLPGLMRALLRAEQVRMGMAAAAAGMGAAA
                     FTSAATRTSPGSVDRVVTASAASGYAQASQQAALLSARYGEAARTRFKATTVSRDFVF
                     MMVKQPKGYGLARVDKQTGRIEAVIDLGRDKDPIYDVDAVSNLIFYRPTPETVCGYRF
                     "
     gene            5681..5998
                     /locus_tag="VFD06_02125"
     CDS             5681..5998
                     /locus_tag="VFD06_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02361.1"
                     /translation="MKTIHRLFTVLALVALVAAPMSATFAGAKKTHDVEVTIVSVNPD
                     GKTMTIKGPDGAEKTVPVTGNAISKLKSVQPGQTVTVTCTDKETGEHEAISDIKVAAE
                     KKG"
     gene            6109..6570
                     /locus_tag="VFD06_02130"
     CDS             6109..6570
                     /locus_tag="VFD06_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019690646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN02362.1"
                     /translation="MPIKLIVNGRPTTVDAPAEMPLLWVVRDLLNLKGTKYGCGMSVC
                     GMCTLHVDGEATRSCVTPVGSVAGKKITTIEGLSSDGSHPVQKAWMDLDVAQCGYCQA
                     GQIMSAAALLAKTAKPTDTQISAAMDGNLCRCGTYLRIREAIRKAAAAAKA"
     gene            6581..8905
                     /locus_tag="VFD06_02135"
     CDS             6581..8905
                     /locus_tag="VFD06_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZN02363.1"
                     /translation="MDTRRSFLKVSALAGGGMLIGLHLAPRGAQGQPQPETARLAPNA
                     FVRIAADGTVTITAKNPEVGQSVKVALPMLIAEELDADWKDVRIEQADLDEAKYGEQF
                     AGGSVAIPNHWLPMRQVGAAARAMLVAAAAKEWKVPAAECSTAAGQVRHAASKRSLPY
                     GALAAKAAALPVPDPETLKLKDPKEYRIIGRPLPGPDNLALVTGKPLFGTDVTVLGML
                     HAVYEKCPVYGGKVVSANLDAVKGMPGVRQAFVVEGGTDLAGLLPGVAIVADSHWAAR
                     QAREKLEVKWDEGPTASQGSEGFARRAEELWKQPPARTLRSDGDAEAALKTAAKTVEA
                     SYTYPFLAHASAEPMNCTARVVEGKVEIWAASQTPGSGRAMVAKTLGISEENVTVHLT
                     RIGSCFGRRLNNDYMVEAAAIARRAGVPVQLLWTREDDIRHDFYRPGGFHALKGGVDA
                     TGKIVAWRDHFVSFGEGERFARGASMSERDFPARFVPHYALVSSVMPLGVPTGALRAP
                     GDNAVCFVVQSFIDELAHAAGKDPLQVRLAMLDAEVVPAPAGNNPLARFNPKRMRGVY
                     EAVAAKSGWGARTLPKGTALGMGGHFCHYGYCAVVAEVRVDASNALRVDKVWTATDVG
                     RPIINPSGAIQQVQGSVVDGLSHLMDFEITFERGRAVQSNLHEFTPLRFSETPAVIDA
                     HFVETDNPPTGLGEPILPPVLPAVPTPSSPPPESASAPCRSRSSATPGPRKPERILST
                     APRAQKSLGGIPPRGRRSIPASKNCFALGQFRST"
     gene            8944..10845
                     /locus_tag="VFD06_02140"
     CDS             8944..10845
                     /locus_tag="VFD06_02140"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZN02364.1"
                     /translation="MHWRIAMRHTGACCVVLLSGIGIVAAAPPGEVTGVQVGSSSQIS
                     WTTIAGAADYNVYRGLVSWLGRGDGAECHGDEIAATSFPSAANPPAGEAYFYLVTAES
                     SSGEEGTAGTGTGGSGRPLRGSCDAIVRHHVLERVGYGGDEWTRGRIAALGNQGYLNE
                     QLDPASIDETTNNELINRRASLVPPDNANELLALDIVNAVYARRQLEQQVTMFWDNHF
                     NTDHHESFDYFNFYSALFPATQNLESAALHYEAQNQFRDLAFNGTFREIVEASGLGRA
                     MIIYLDTISNIATAPNENYPRELLELHTMGVDGGYTQQDIVQLAKVFTGWNICKKDAS
                     VAADPLAACIPSNTYGTATEPPGLWVVNFRTSRHDTTQKTLFAGTPYQRIIPSTAGNP
                     AAGIADAQLAFDAIVAHPSTPRFIAKKLLQRFVDEQPTQTMIDAVVLAWNNPANPHGI
                     GDLREVLRAVLAQAAFRDPDRAGSKIKSPFEQVVSALRAVRGMTDGQSSVRNYVNRMS
                     ELFHQNPVPTGFPEIGADWLDTNNLLERQNFGFDMATRTATSFGADVIGLLNAAGVST
                     APTPNNAPAIVDYLAGVLYGGALTASERQRAIDFLNTNDSGVVSNYTDTRIRETAGFM
                     MGFAQFLEQ"
     gene            10856..12265
                     /locus_tag="VFD06_02145"
     CDS             10856..12265
                     /locus_tag="VFD06_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN02365.1"
                     /translation="MSRHVDCDCNRRSFLRGAGLTLAGFGAASLLPGPFLRQAMAATA
                     GPFSNRRLIFIFLRGGNDGLNTVIPGGDPDYNAVNRPTLYIPNASSLDLGNGFARLHP
                     AMSALLPLYGSGDLAIVHRVGYPNNTMSHFDDQRVWENGDPAQKQLFEGWLYRYIEEN
                     AVAAGVNLPVLSVQPNQPILLRGDEPFVNIANPNSFDYLPSDPKRTKLKNGWDAAYDN
                     LTGLEPYRPLLSQTSVKLIDTLDEYRSWSQLTWDPKDPNTGFSLFPVSDATNPNFPPG
                     MFPSSSYSFFRALKICALSMLESDVVNNNGTRVAGCELGGFDTHDTQGATAGTQATLL
                     AQLAYGLKSLQIVLSGGANDPRAYPAVWNDTVVCTLSEFGRTTKENGSVGTDHAVASC
                     LFIAGGTVNGGVYNCDPSTWPAGVMFAKDGRYLSTRTDYRAIFWEILRDHMGADPVKV
                     NTVFPGYTGLGLTELGLIA"
     gene            12262..>13835
                     /locus_tag="VFD06_02150"
     CDS             12262..>13835
                     /locus_tag="VFD06_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02366.1"
                     /translation="MRPGRIAPVLIAAALLGGAAAALSPDPPALTVSPPRGTPPATIT
                     LSGRPLDPDAKVALLGGGPFVAGAFLLPEGARSLQVRDDRVSVAFYSHTSKLGGIQTI
                     DFRDPAHPVRVGGFETGDSGIGVEIRGDLAYIAFLNPYTFLGGLHVVDLAPPGGAVRL
                     GSFYTFLDPQAMVLDDRHVYVADGATGLRIVDVTDPEAPVEAATCPSHGTAHDVALGS
                     GRAYVADGVGGLRILDIADPRAPLEIGAWAPPGADLLSVALDGSLAFVTDLKRGLLVL
                     DVSEPHEASLLAEMPLPDAANGLARSGHRLYAAAGASGLQIVDVGDPHQPRVIGSRGF
                     AGNGGYFFDVAVEGDRAYVADLLNGLVVIDVGRPESPTLAGGADLPGLTASVATDAGL
                     AVAGGDSGVAVLEMDSGGGLLPAGRLPTSAPVLGIAAAGGHAFAAAGADGLLVVDLTD
                     PRQPSVVASVDTPGEARGIVLGDGKAFVADGSRGLAIFDVADPTAPAPLGSLDTQGTS
                     RGLAVEGSLVYVADDFR"
BASE COUNT         2211 a         4920 c         4700 g         2004 t
ORIGIN      
        1 atgtagccga ccgtccccat gatcacaccg gcctcggtcg aggccggtgt cgtcggcagg
       61 ctcgtgaccg ggccggcctg gacatccggc tggagcagct tcgcgagacc gaagtcgagg
      121 atcttgatcc ggccgtcgcg cgtgatgaag agattctccg gcttgaggtc gcggtgcacg
      181 atgccgcgct cgtgcgccgc cgccaggccg cgcgccgcct gggccgccag gtcggtcgcc
      241 ttgcggaccg gcagcggccc ctgcgccacg cgctcgcgca gcgtctcccc ttccaggagc
      301 tccgacacca ggtacggcat cccctcgtgc gacccgatgt cgtggatggc gaggatgttc
      361 gggtggttga gcgcccccgt cgcgcgggct tcctgctcga agcgcgccag ccggtcggca
      421 tgcaccgcga acgcggccgg caggaccttc accgcgacgt cccgccccag ccgcgcgtcg
      481 cgcgcccgat agacctctcc catgcccccc gcgccgagcg gggcgatcac ttcatagggg
      541 ccgagtcggg ttcccggggc gagcgccatg ggcggcgcgc attgtagcgc ggcggccgtt
      601 ctgtccgtgg gacctacgcg gcgggccccg ggggagactc gagccacgcg agggccgcgg
      661 cgcgctcgcc ggccttgaag ttccggatct ccgcattcac gaaatggctg gcgatcttcg
      721 gcgccgcgga gagaagagcg ctgtccgaga cgaccgcgat acggcggatg cgtttgtgat
      781 gatcgcgcac gaagcgcagg tgcgagacga gcgcggcgaa gtcgtcccag ccggggaacg
      841 acggggcgtc gaccaccagc cccttcagat ccccgtgctc ttcgaggtac gggtcgaccg
      901 ggccggcgag agccgtgaag tccgcggcgg tgagcgcccc ccgcggttcg aggatcagga
      961 tgccccggtc acgcagcagc tcgaaatgaa tcatgccgtc cctcccctgg gagtatcccg
     1021 ccggtcacga ctctcgcaca tgacgccggc cgattccacc cgggacgccc cggtcaccca
     1081 ggaacgacgg acgccgattt gcggtgcacg cgcggtcagt gcgccggccg ccggctccgc
     1141 agcaccgcga cgttccccgc gatgcacagc aggatgccgg cggcgagcga cggatgccag
     1201 cgcagcccct cgaagagtcc cgacaggagc agcgccacca ccgggatggc cacggtgatg
     1261 tagccggccc ggtcggcgcc gatccggccg aggagcacga ggaacgcgcc gaacgccacg
     1321 atcgagccga agatggcgag atacccgagc gacacgaggt aacgggccga gaggtcgaac
     1381 cgccacggct ggcgcgtcgc gagcgcatac gctccgatac acagcccgcc atagaccatg
     1441 ccgaaggtgt tcatcggcac gaccggcaga ccgcgcgcgt ggttgcggta ggccacgagg
     1501 ttcccgagcg aggcgctgac ggcgccggcg agagcgaggg cgatgccgag tccggcgctc
     1561 ccgcgcgccg tcccaccgcg aatctcgggg aggaagacga gcgccacccc ggcgatcccg
     1621 agagcggcgc cgagcatcac cgccggcttc agcggcgtgc cgaaaaagag gcggctcccg
     1681 acgatgttga gcaccgtcag gagcgagaag gccacggcca ccaggcccga ggtgatgtgg
     1741 gtctccgcca ggtagacgca gaggtagttg atcccgaaca ggagcactcc ctggagcgcc
     1801 atccaaaggt gctcccgggc cgagaagcgc aggcggatcc gcttgagcgc cgaccaggcg
     1861 aacaggatcg ccccggcgag gaggaagcgg tacaccaccg acaccgccgg aggcaccgtc
     1921 ccgaactgga acgtgatggc gagccacgtc gatccccaga tgaggaccga gatcgcgtag
     1981 agaccgaggc tgctcaaggg cgcgcccagg cgctgccgaa ctggatgtag agcgcgctgt
     2041 cgtccggccc ccaggcgacg tccgcgccga cgtgcagtcc gtacttgcgc gccagctcgt
     2101 accggacgcc agtgccgccc gtgacgatcg acttcacgtc ctcggtctcc tcgaggtcgt
     2161 tccacgcggc gccggtgccg acgaagccga cgacgctcca gcgcttccag cactgccagc
     2221 ggatctccgt ctcaatctcg gccgcctcct ccccctggta gcgcatcacc ggggcgccgc
     2281 gcagggtgat gaacggcagc atgtaaaagg ggggatcgct gaacgagacg gtcagcccgc
     2341 tgcgcactcc gaagaagaca cgctggtgca gcggacggta gacgatccag gtcagggcga
     2401 cgcgctggaa cgacgtgtcc cctcccagcg attcgtcgaa gacgccggtc gagccctcga
     2461 agtaggtgcc gcgcgtcggc gtgaagatgt tgtcgcgcga atcgaacgcg atcgacggcg
     2521 tcagcccgcc gatccgggag tcctcgggcg gatcgaggag cgccgccgcc agcgactcgt
     2581 cgtcgaagga gatgtgggtg tcggccaggg cgtaactcat cccgacccac aggcgcgacg
     2641 ccccgaggcg gtagcggcca cgcaccccgc cccccagcgg ctccagggtg taatgcagcg
     2701 ggtcgtgctc gagcgtctca tcctttccga tgccgtagaa gtcgaggttc accttcgcgt
     2761 cgaggatggc ggcgaccgtc tgcacccgat cgtccatcca ctgcctgacg tcccccgcca
     2821 tcaccgcgcg cgtgccgttc tcggtcgccg ccccgccgac gaaggacaga ttcggccggc
     2881 cgaaccccgc cttcgccgac tcggcgatag gccggttgat gaagacgcac cccagcgcgc
     2941 ccccgtaccc gaccgccggt tcggtgatcg gcacgaggat cggcacgaac ccgtacgcct
     3001 tgtcgaggaa cgagctgacg tcgaactcac cgtcctcttc ggagcgcaat ttcggtttcg
     3061 gcgtcgccgt cgtggaagac ccttcggcag catcctccgc atcggcatcg ccggcggcgc
     3121 gcaccgggct gccggcgagc gccgcgccga ggcacgcgat cgccatccac gcagcgattg
     3181 gcttgcggag ccatcgaatg gagcgtgcgg gtcgcttctc ggtcgtggtg cggctcctct
     3241 tccggggcgg ggattatatg cagcgcgacg cccgaagcgg gcggcggcgg tcgcaactca
     3301 tagaggatag ggggtcggtt gcgattccgt catcgtgcgg ccttcccgtg catcgcgccc
     3361 taaattgtcc cggcgcccgg accgttgcgg cgcgggagat tctccatgcg ttctcgactt
     3421 cgcaccctcg gcggtgcctc cgccgtcctg gccgttctgc tcgccctgca gcccggcgcg
     3481 gggatcgccg cccccgcggc cgctccggcc gcgcccgctg cttcggccgc cgcggcgccg
     3541 gtcctctccc ccgcctggag cgcgcgtctc gacggcatgg tcgagtggca gcgggtggcg
     3601 ccgctcggcc agttgctggt caagacgacc ctgggcctca cgggcttcga tcccgagcgc
     3661 ggccgggcga tctggaagca gccggggctc ggagggatgg cgcaggatca ttacgaggag
     3721 atcgacggca cctcgctcgt cgtcctttcg gacgggttgg cgaagccgcg cgtcgtcatc
     3781 ctcgactcgg tggacggccg gatcctcttc gactcacggg cgtacggcgt cgcccaggtc
     3841 ctgagccggc actacctgcc gaagagccgc gccctcctcc tcttcggctt cagcgagggg
     3901 gacccggcga cgacgatgta cctcgccgac gtggaccgcg gcacgatccg ctggaagaac
     3961 aaccggctcc tggaggggca ggggaagttc acccgcgccc tcacctcctt cctgcaggcc
     4021 gcgacgaacc agtccggcat catctcggat ccgctcgaaa tcgcctccga caccttcgtc
     4081 atcgcctcga ccaccgagat cctcgcggtg cggacggcca ccggggagat cgcctggcgc
     4141 gtcccgaacg tgcacaacac gcgatcgacg cgcttccatg ccgacgaggg gcgcgccccc
     4201 ggtctgctgt tcgtcggcag cgagacggcg ttcacctcga tgacctcgac cgccggcgcg
     4261 cagaagagcg aaacgattta cagcgagtac tccgcgcggc ggatggcgga cggcgcggcg
     4321 gcgtggggga agccggtgcg ggtcaaggga ggcctcaacg acgtcatctt cgccgaccgc
     4381 ggcctcgtcg tcagcccgcg gaccaccggc aaggggaaga tcctcctctg cgactaccgc
     4441 tccggggagc cgctgtgggg gaagaagggg aaagggatcg acatcaccgg cggcatcatc
     4501 aatcacgact ggacccgcgc cggcctcgtg ctgaccaccg gctacgacag cgcctggagc
     4561 aacaaaggga ccgagtactt cctgacgctc gtcgatccgg ccaccgggct gccgcgcttc
     4621 gaggagccgc tgcggttgcg cggccgcgtc gtctcgacgc aggtcctgcc ggcggggctc
     4681 ctgtacacga cgacgagcga ggtgaacatc ctcgatttga agaccggaca gccgctgctc
     4741 ggggacgggg tgcgttcgga cgagtccctg gtcaccacga ccgccggctc gtccctgttc
     4801 gcctacgctg gaaagcacgg gacgctgcac cggctcgacc tcgaccgcgc ggcgctctcg
     4861 acggtgtcat cggcgcccga gcggctcgag gaggacgagg caccgatggc gatcgaggcc
     4921 gacggcaacc ggatcaccgt tctttcgagc cagaacgtca tcgcctggaa cgcggacggc
     4981 tcgctgctgt tccacgccta ccatccttcg ccgcgcctgc cggggctgat gcgggcgctg
     5041 ctgcgcgccg aacaggtgcg catggggatg gccgccgctg cggccggcat gggggcggcc
     5101 gccttcacct cggccgcgac ccgcacctct cccggctcgg tcgatcgggt cgtgaccgcc
     5161 tcggccgcca gcggctacgc gcaggcgtcg cagcaggcgg cgctcttgag cgcgcgctac
     5221 ggcgaggcgg cgcgcacccg cttcaaggcc acgacggtct cccgcgattt cgtcttcatg
     5281 atggtgaagc agccgaaggg gtatggcctc gcccgcgtcg acaagcagac cggccgcatc
     5341 gaggcggtca tcgatctcgg ccgcgacaag gatccgatct acgacgtgga cgccgtctcg
     5401 aatctgatct tctaccgccc gaccccggag accgtctgtg gctatcgctt ctgaaggctc
     5461 cggcgaccgc gtccgccgcg ctcctgggag ccttcgccgg ccacgccaaa accgtaatcg
     5521 cggccccgcg gcccgcccct gtctcttcct gaaggcttcc tgaagaaatc gacccgcggg
     5581 tggttgaaac cgccttcggg gtgtacccta cggcgtttcc tacatcgacg ccggggggcc
     5641 tcgttcgagg gatcccggcg gccaagagga gggtccgagg atgaagacga ttcatcggct
     5701 gttcacggtg ctcgcgctcg ttgcgcttgt cgcggcgccg atgtcggcga cgttcgccgg
     5761 cgccaaaaag acccacgatg tcgaagtcac tatcgtctcc gtcaatcccg acggaaagac
     5821 gatgacgatc aagggaccgg acggcgctga gaagaccgtt cccgtgaccg gcaatgcgat
     5881 cagcaagctg aagagcgtgc agccgggaca gacggtcacc gtcacctgca ccgacaagga
     5941 gaccggcgag cacgaggcca tcagcgacat caaggtggcg gccgaaaaga agggctgaga
     6001 tcggctcttc gaagtcccga tccggggcgt ccggaatcct tccggacgcc ctgttttttt
     6061 ttcgggccgg tgtaggatgt ccccctacca cgcaccggga gtgacgccat gccgatcaag
     6121 ctgatcgtga acgggcggcc gaccacggtg gacgccccgg cggagatgcc gctgctctgg
     6181 gtcgtccgcg accttctcaa cctcaagggg acgaaatacg gctgcggcat gtcggtgtgc
     6241 ggcatgtgca ccctgcacgt cgacggcgag gcgacccgct cctgtgtcac gcccgtgggg
     6301 agcgtcgcgg ggaagaagat caccaccatc gaggggctct cttcggatgg ctcgcacccg
     6361 gtccagaagg cctggatgga tctcgacgtc gcccagtgcg gctactgcca ggccggtcag
     6421 atcatgtcgg ccgcggccct tctggcgaaa acggcgaagc cgaccgacac ccagatcagc
     6481 gcggcgatgg acgggaatct ctgccgctgc gggacctacc tgcgcattcg cgaggcgatt
     6541 cgcaaggcgg ccgcggcggc gaaggcgtaa gaggatcgcc atggacactc gccggtcgtt
     6601 cctcaaggtc agcgcactcg ctggaggggg catgctcatc ggcctgcacc tggcgcctcg
     6661 cggggcgcag ggccagccgc agccggagac ggcgcgtctg gccccgaacg cctttgtgcg
     6721 catcgccgcc gacggcaccg tgaccatcac cgccaagaac cccgaagtcg gccagtcggt
     6781 gaaggtggcc ctgccgatgc tcatcgccga ggagctggac gccgactgga aggacgtccg
     6841 catcgagcag gcggacctcg acgaggcgaa gtacggcgag cagttcgccg gcggcagcgt
     6901 ggcgatcccg aaccactggc tgccgatgcg ccaggtggga gccgcggcgc gggcgatgct
     6961 cgtggccgcc gccgcgaaag agtggaaggt gcccgccgcg gagtgctcca ccgccgccgg
     7021 acaggtccgc cacgccgcgt cgaagcgctc gctcccctac ggcgcgctcg cggcgaaggc
     7081 ggcggcgctc ccggtccccg atccggaaac gctgaagctc aaggacccga aggagtatcg
     7141 gatcatcggc cggccgctcc ccggacccga caacctcgcc ctcgtgaccg gcaagccgct
     7201 cttcggcacc gacgtcacgg tgcttgggat gctgcacgcc gtctacgaga agtgtccggt
     7261 gtacggcggc aaggtcgttt cggccaatct cgacgccgtc aagggaatgc ccggggtgcg
     7321 tcaggcgttc gtcgtcgaag gagggaccga tctcgccggt ctcctcccgg gcgtcgccat
     7381 cgtcgcggac agccactggg cggcgcgcca ggcccgcgaa aagctcgagg tcaagtggga
     7441 cgaaggaccg accgcgtcgc agggcagcga agggttcgcc cgccgcgcgg aggagctgtg
     7501 gaagcagccg ccggcccgca cgctgcgatc cgacggggac gccgaggcgg ccctgaagac
     7561 ggcggcgaag acggtcgaag cctcgtatac gtatccgttc ctggcgcacg cttcggccga
     7621 gccgatgaac tgcaccgccc gcgtcgtcga ggggaaggtg gagatctggg ccgcgtccca
     7681 gacgccggga tcggggcgcg ccatggtggc caaaaccctc ggcatctccg aagagaacgt
     7741 caccgtccac ctgacccgca tcggatcctg cttcggacgg cggctcaaca acgattacat
     7801 ggtcgaggcg gccgccatcg cccggcgggc gggggtgccg gtgcagctcc tctggacgcg
     7861 cgaagacgac atccggcacg acttctaccg cccgggcggt ttccacgcgc tcaagggggg
     7921 cgtggacgcc accgggaaga tcgtcgcctg gcgcgatcac ttcgtctcct tcggcgaggg
     7981 ggagcggttc gcgcgcggtg cgtcgatgtc ggagcgggac ttccccgccc gcttcgtccc
     8041 gcactatgcg ctcgtctcct cggtcatgcc gctcggcgtg ccgaccgggg cgctgcgcgc
     8101 cccgggcgac aacgcggtct gtttcgtggt gcagtcgttc atcgacgagc tggcgcacgc
     8161 cgccggcaag gatccgctcc aggtgcgact ggccatgctc gacgccgagg tcgtcccggc
     8221 gccggcgggc aacaatcccc tggcgcgctt caacccgaag cggatgcgcg gcgtgtacga
     8281 ggcggtggcg gcgaaatcgg gctggggcgc ccgcacgctc cccaagggga cggcgctcgg
     8341 catgggcggc cacttctgcc actacggcta ctgcgccgtc gtggcggagg tgcgcgtcga
     8401 cgcgagcaac gccctgcgtg tggacaaggt gtggaccgcg accgacgtcg gccgtccgat
     8461 catcaacccg agcggcgcca tccagcaggt gcaggggtcg gtcgtggacg gcctgagcca
     8521 cctgatggac ttcgagatca ccttcgagcg cggtcgggcg gtgcagagca acctgcacga
     8581 gttcacgccg ttgcggttct cggagacccc ggctgtcatc gacgcccatt tcgtcgagac
     8641 cgacaacccg ccgaccgggc tgggcgagcc gatcctcccg ccggtcctgc cggctgtgcc
     8701 aacgccatct tcaccgccac cggaaagcgc atccgctccc tgccgctcgc gaagctcggc
     8761 tactcctggg ccacgaaagc ctgagagaat cctgagcacg gcgcctcggg cgcaaaaatc
     8821 cctgggcggc atcccgccac ggggtcgcag atcgatcccc gcatcgaaaa actgctttgc
     8881 gctcgggcaa ttccgcagta catagaggct tccgtccctc ccagacagga taaggagacg
     8941 tccatgcact ggcggatcgc catgcggcac acgggcgctt gctgcgtcgt cctcctgagc
     9001 gggatcggga tcgtcgcggc cgcgcctccg ggggaagtga ccggggtcca ggtcggatcg
     9061 tcctcgcaga tctcctggac gaccatcgcc ggggccgccg actacaacgt ctaccgcggg
     9121 ctcgtttcgt ggctcggccg cggggacggc gccgagtgcc acggcgacga gatcgccgcg
     9181 acctcgttcc cgagcgccgc gaatccgccg gcgggcgagg cctacttcta cctcgtgacc
     9241 gccgagtcga gctccgggga ggaagggacg gccggcaccg gcacgggcgg gagcggccgg
     9301 ccgctgcgcg gctcctgcga cgccatcgtg cgccaccacg tcctcgaacg ggtcggctac
     9361 ggcggcgacg aatggacccg cggccgcatc gccgccctgg ggaaccaggg ctacctgaat
     9421 gagcagctcg atccggcctc catcgacgag accaccaaca acgagctgat caaccgccgc
     9481 gcctccctcg tgccaccgga caacgccaac gagctgctgg cgctcgacat cgtcaacgcc
     9541 gtgtacgccc ggcggcagct cgagcagcag gtgacgatgt tctgggacaa ccacttcaac
     9601 accgatcacc acgagagctt cgactacttc aacttctact cggcgctgtt cccggcgacg
     9661 cagaatctgg agtctgcggc gctccactac gaagcccaga accagttccg cgatctggcg
     9721 ttcaacggca cgttccggga gatcgtggaa gcgagcggcc tcggccgcgc gatgatcatc
     9781 tatctcgaca ccatctcgaa catcgccacc gcccccaacg agaactatcc ccgcgagctg
     9841 ctggagctgc acaccatggg ggtggacggc ggctacacgc agcaggacat cgtgcagctc
     9901 gccaaggtgt tcaccggctg gaacatctgc aagaaggacg cgtcggtggc ggccgacccg
     9961 ctcgccgcct gcatcccgag caacacctac ggcaccgcga ccgagccccc cggtctctgg
    10021 gtggtcaatt tccgcacctc gcgccacgac accacgcaaa agacgctgtt cgcgggaacg
    10081 ccgtaccaga gaatcatccc gtccaccgcc ggcaacccgg cagccgggat cgccgatgcg
    10141 cagctggcgt tcgacgccat cgtggcccac ccctcgacgc cgcgcttcat cgcgaagaag
    10201 ctcctgcagc gcttcgtgga cgagcagccg acccagacga tgatcgacgc cgtggtcctg
    10261 gcctggaaca accccgccaa cccgcacggc atcggcgacc tgcgcgaagt gctgcgggcg
    10321 gtgctggcgc aggcggcgtt ccgcgatccg gatcgcgcgg ggagcaagat caagtctcct
    10381 ttcgaacagg tggtctcggc gctgcgcgca gtccggggga tgaccgacgg ccagtcctcg
    10441 gtgcgcaact acgtgaaccg catgtccgag ctcttccacc agaacccggt tccgaccggc
    10501 ttcccggaga tcggcgccga ctggctcgac accaacaacc tcctcgagcg gcagaacttc
    10561 ggcttcgaca tggcgacgcg caccgcgacc agctttggag ccgacgtcat cgggctcttg
    10621 aacgccgccg gcgtctccac ggcgccgacg cccaacaacg cgccggccat cgtcgattac
    10681 ctcgccgggg tcctgtacgg cggcgcgctc accgcatcgg aaagacagcg cgccatcgac
    10741 ttcctcaaca ccaacgacag cggcgtcgtg agcaactaca ccgacacccg gatccgcgag
    10801 acggccgggt tcatgatggg cttcgcccag ttcctcgagc agtaggaggc gcccgatgag
    10861 ccgacacgtg gattgcgact gcaaccgacg ctccttcctg cgcggcgccg gactgacgct
    10921 cgccggcttc ggcgcggcgt ccctcctgcc gggccccttc ctgcggcagg cgatggccgc
    10981 gaccgccggt ccgttcagca accgccgcct catcttcatc tttctgcgcg gcggcaacga
    11041 cggcctgaac accgtcatcc cgggcggcga cccggactac aacgcggtca accgcccgac
    11101 cttgtacatc ccgaacgcct cctccctcga tctcgggaac ggcttcgcgc ggctgcaccc
    11161 cgcgatgtcg gcgctgctgc cactctacgg ctccggcgac ctggcgatcg tccaccgcgt
    11221 cggatacccg aacaacacca tgtcccactt cgacgaccag agggtctggg agaacggcga
    11281 tccggcgcag aagcagctgt tcgaaggctg gctgtatcgc tacatcgagg agaacgcggt
    11341 cgcggccggt gtgaacctgc cggtgctgtc ggtgcagccg aaccagccga tcctgctgcg
    11401 gggcgacgag ccgttcgtca acatcgccaa cccgaactcc ttcgattacc tcccctcgga
    11461 ccccaagcgg accaagctga agaacggctg ggacgccgcc tacgacaacc tgaccggact
    11521 cgagccgtac cgcccgctgc tgtcgcagac gagcgtcaag ctgatcgaca cgctcgacga
    11581 ataccgatcg tggagccagc tgacgtggga cccgaaggat ccgaacaccg gcttctccct
    11641 cttcccggtc agcgacgcga ccaacccgaa tttccctccg gggatgttcc cgtcgtcgtc
    11701 gtacagcttc ttccgcgcct tgaagatctg cgccctgtcg atgctggaat cggacgtcgt
    11761 gaacaacaac ggcacccgcg tggcgggatg cgagctgggg ggattcgaca cccacgacac
    11821 acagggggcc accgccggca cgcaggcgac cctgctggcg cagctggcgt acggcctcaa
    11881 gtcgctgcag atcgtgctgt cggggggagc gaacgacccg cgcgcctacc ccgcggtctg
    11941 gaacgacacg gtcgtctgca ccctgtcgga gttcggccgc accaccaagg agaacggcag
    12001 cgtcggaacc gatcacgccg tcgcgtcctg cctgttcatc gccgggggca cggtgaacgg
    12061 cggcgtctac aactgcgacc cgtccacctg gccggccggg gtcatgttcg cgaaggatgg
    12121 ccgctacctg agcacccgca ccgattaccg ggcgatcttc tgggagatcc tgcgcgatca
    12181 catgggggcc gatccggtga aggtgaacac ggtcttcccc ggatacaccg gcctcggcct
    12241 gaccgagctc ggtctcatcg cgtgagaccc gggaggatcg cgccggtcct catcgccgcg
    12301 gcgctcctgg gaggggcggc ggcggcgctc tcaccggatc cgcccgccct caccgtgagc
    12361 ccgccgcgcg gcacgccgcc ggcgacgatc acgctgtcgg gacgtcccct cgatccggat
    12421 gcgaaggtgg cgctgctcgg gggcgggccg ttcgtggccg gcgccttcct ccttccggag
    12481 ggcgcgcgct ccctgcaggt gcgcgacgat cgcgtttcgg tggcgtttta cagccacacc
    12541 agcaagctcg gcggcatcca gacgatcgac tttcgcgacc cggcgcaccc ggtgcgcgtc
    12601 ggcgggttcg agaccggcga cagcggcatc ggcgtcgaga tacggggcga cctcgcctac
    12661 atcgcgttcc tgaacccgta caccttcctc ggagggctgc acgtcgtcga tctggcccca
    12721 cccggcgggg cggtgcgtct ggggtcgttc tacacgttcc tggatccgca ggcgatggtc
    12781 ctcgacgacc ggcacgtcta tgtcgccgac ggggccaccg gcctgcggat cgtcgacgtg
    12841 accgacccgg aagcgccggt cgaggcagcg acctgtccgt cgcacggcac ggcgcacgac
    12901 gtcgccctgg ggtccggccg ggcgtacgtc gccgacggcg tgggagggct gcgcatcctg
    12961 gacatcgccg atccacgggc gcccctcgag atcggtgcgt gggcgccgcc gggtgccgac
    13021 ctcctgagcg tcgcgctcga cggctccctc gccttcgtca ccgacctgaa gcgcggcctg
    13081 ctggtgctcg acgtctccga acctcatgag gcgtcgctcc tggccgagat gccgctcccg
    13141 gacgcggcca acggcctggc gcggagcggc caccgtctct acgcggccgc cggcgccagc
    13201 ggtctgcaga tcgtcgatgt cggcgacccg caccaaccgc gggtgatcgg cagccggggg
    13261 ttcgcgggca acggcgggta cttcttcgac gtcgccgtgg agggagatcg ggcgtacgtg
    13321 gccgacctgc tcaacggcct cgtggtgatc gacgtcgggc ggccggaatc accgacgctc
    13381 gcgggcggag ccgaccttcc gggcctcacg gcgtcggtgg cgaccgacgc ggggctcgcc
    13441 gtggccggcg gcgacagcgg cgtcgcggtg ctcgaaatgg actccggcgg cggcctcctg
    13501 ccggccgggc ggctgccgac ctccgcgccg gtgctcggga tcgccgccgc cggaggacac
    13561 gccttcgcgg cggccggcgc cgacggcctg ctcgtcgtcg acctcacgga cccgcgacag
    13621 ccgtcggtcg tcgcgtcggt cgatacgccc ggggaggcgc gggggatcgt gctgggggac
    13681 ggcaaggcgt tcgtcgccga cggatcgcgc ggtctggcga tcttcgacgt cgcggacccc
    13741 acggctcctg cgccgctcgg ctcgctggac acgcagggga cgtcgcgcgg cctggcggtg
    13801 gagggatcgc tcgtctacgt cgccgacgat ttccg
//
LOCUS       DASRIQ010000085         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_287850, whole genome shotgun sequence.
ACCESSION   DASRIQ010000085 DASRIQ010000000
VERSION     DASRIQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287850"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /gene="groEL"
                     /locus_tag="VFD06_02155"
     CDS             complement(<1..769)
                     /gene="groEL"
                     /locus_tag="VFD06_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZN02367.1"
                     /translation="MPKQLLFEEEARAALLRGVNIMSRAVTVTLGPKGRNVVIAKKYG
                     SPAMTKDGVTVAKEIELKDPAQDMGAQMLKEVAAKTSDIAGDGTTTATVLAQAIFRGG
                     LKNVTAGANPMSLKRGIEQAVDTVVAELKHMSKPTKDKNEIAQVATIASNNDKTIGNL
                     IAEAMEKVGKDGVITVEEAKAMETTLEVVEGMQFDRGYLSPYFVTDPERMECVLEDAL
                     ILIYEKKLSVMKDMLPLLEQVARAGKPLLIVAEDLEGE"
     gene            complement(825..1133)
                     /locus_tag="VFD06_02160"
     CDS             complement(825..1133)
                     /locus_tag="VFD06_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZN02368.1"
                     /translation="MVQAIQTTLRPLHDRVLVKRLEEGDAKRGSIIIPDTAKEKPQEG
                     KVVAVGAGTVTDEGKNRPLAVKKGDRILFGKYSGSEIQLNGEDFLIMKEEDVLGILSA
                     "
     gene            1253..2314
                     /locus_tag="VFD06_02165"
     CDS             1253..2314
                     /locus_tag="VFD06_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN02369.1"
                     /translation="MKIPLNLHVDARSPIPIRRQLTEQLKHVIESGGLPQNQTLPSIR
                     ELAGFLGINANTVARVIEDLKRSGYVEARRGSGVFVASAPPVRPSPRLREGFLHDAVM
                     RAAALGMTPDDLAVGVLSLGGVTPPAIDGAVEVLLVECSPGELDFFAGQLEAHLPIRV
                     DKVLLGELAMVTRRPAQAGRWRAAVTSFCHLPEVERVLKGRGVPVIALLAEAHLETLH
                     RLAQLPAGTRVGVASDAVETAHNLEHSIANAALPNIVLAGACPAEEAALRRLVRRVDV
                     VVCSSTAAERVRKLADPAVQVMIDDRALDQRAIQMLAVLLVRQNGGGTAAEPPTAQKR
                     RSDRRRPPQSARRAAERTK"
BASE COUNT          349 a          764 c          779 g          441 t
ORIGIN      
        1 cctcgccttc cagatcctcg gcgacgatga ggagcggctt gccggcccgg gcaacctgct
       61 cgagcagggg cagcatgtcc ttcatcacgc tgagcttctt ttcgtagatc aggatgaggg
      121 cgtcctcgag cacgcactcc atgcgctcgg ggtcggtgac gaagtagggg gagaggtacc
      181 cgcggtcgaa ctgcattccc tcgaccacct ccagggtggt ctccatcgcc ttcgcctctt
      241 cgacggtgat gacgccgtcc ttgccgacct tttccatggc ttcggcgatc agattgccga
      301 tcgtcttgtc gttgttcgac gcgatcgtcg caacctgcgc gatctcgttc ttgtccttgg
      361 tgggcttgga catgtgcttc agctccgcga ccaccgtatc gaccgcctgc tcgatccccc
      421 gcttcaggct catcgggttc gcgccggccg tcacgttctt gaggccgccg cggaagatgg
      481 cctgggcgag caccgtcgcg gtggtggtac cgtcccccgc gatgtcggac gtcttcgcgg
      541 cgacttcctt gagcatctgc gcgcccatgt cctgggcggg gtccttgagc tcgatctcct
      601 tggcgacggt gacgccgtcc ttggtcatgg cagggctgcc gtacttcttc gcgatgacga
      661 cgttccgccc cttcggcccg agcgtcaccg tcacggcgcg cgacatgatg ttgacgccgc
      721 gcaagagcgc ggcgcgggct tcctcctcga acagaagctg cttgggcatg gaataccttc
      781 cttccgggta tgcacgaatg gttgaccgac gcgacgcggg gtgtctatgc gctgaggatg
      841 ccgaggacgt cctcctcctt catgatgagg aagtcctcgc cgttcagctg gatctcgctg
      901 ccggagtact tgccgaagag gattcggtcg cctttcttca ccgcgagcgg gcggttcttg
      961 ccctcgtcgg tgacggtgcc ggcgccgacg gcgaccacct tgccctcctg cggcttttcc
     1021 ttggcggtgt cggggatgat gatgctcccc cgcttcgcgt cgccctcctc gaggcgcttg
     1081 acgagaacac ggtcatggag cggacgtagc gtcgtctgga ttgcctgaac catcggatgc
     1141 cccctcttcg cggctgtggt cagcgcggct ggtactgcgt gcgaagtaac tgtaccacta
     1201 aaatagtaca gtatgacaat ttatctaggt gtactattcg agtcgtacag tgatgaagat
     1261 tccgctcaac ctgcacgtcg acgcacgcag cccgatcccg atccgtcgcc agctgacgga
     1321 gcagttgaag cacgtcatcg agagcggtgg tcttcctcag aaccagacgc tgccgagcat
     1381 ccgggagctg gccggctttc tcggaatcaa tgcgaacacg gtggcgcgcg tcatcgagga
     1441 tctcaagcgg agcgggtacg tggaggcgcg gcggggcagc ggcgtgttcg tcgcttccgc
     1501 accaccggtc cgcccgtcgc cccggctccg tgaaggattt ctccacgacg cggtgatgcg
     1561 ggcggccgcc ctcgggatga cccccgacga cctggccgtg ggcgttctga gccttggcgg
     1621 cgtcacgccc ccggccatcg acggggccgt cgaggtcctg ctcgtcgaat gtagcccggg
     1681 ggagctcgac ttcttcgccg ggcagctcga ggctcacctt ccgatccggg tcgacaaggt
     1741 gctactcggc gagctggcca tggtgacccg gcgcccggcg caggccggac gctggcgggc
     1801 cgcggtcacg agcttctgcc atctcccgga ggtcgagcgg gtgctgaaag gtaggggagt
     1861 gccggtgatc gcgctgctgg ccgaggcgca tctcgagacc ctccaccgcc tcgctcagct
     1921 tccggcgggg acgagggtcg gggtggcgtc cgacgccgtc gagacggcgc acaacctgga
     1981 gcactcgatc gcgaacgctg ccctgccgaa catcgtgctg gccggagctt gccccgccga
     2041 ggaggcggct ttgcgccgtc tggtgcgacg ggtggacgtc gtcgtgtgct cgagcacggc
     2101 ggccgagcgg gtgcgaaagc tcgccgaccc cgccgtgcag gtgatgatcg acgacagggc
     2161 gctggaccag cgggccattc aaatgctcgc agtcctcctg gtgcgacaga acggtggcgg
     2221 gacggcggcc gagcccccaa ccgcccagaa gcgtcggtcc gaccggcgtc ggccaccgca
     2281 gagcgcgagg cgcgcagccg agcgaacgaa atgatccggc tcgacagaac gaa
//
LOCUS       DASRIQ010000086         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2116216, whole genome shotgun sequence.
ACCESSION   DASRIQ010000086 DASRIQ010000000
VERSION     DASRIQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116216"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFD06_02170"
     CDS             complement(<1..462)
                     /locus_tag="VFD06_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN02370.1"
                     /translation="MDEPINILVVDDDPAIREVLSEGLTECGYRCDTAEDGCAALEHL
                     RRSPCRLLISDVDMPRLDGVGLLKQVKAVAPDTAVILLTGMHDFDTALQSMRLGASDY
                     LTKPFHLEQVRLTVERVLETQRLVRENREHHERLEARVLERTSELQRKTLEI"
     gene            complement(608..3322)
                     /gene="polA"
                     /locus_tag="VFD06_02175"
     CDS             complement(608..3322)
                     /gene="polA"
                     /locus_tag="VFD06_02175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZN02371.1"
                     /translation="MGVRPDPSRALYLIDGSNNLYRAFHAIRELRNSRGLPTNATYGF
                     TGMLRKLLKDFQPHALAVLFDTPEPTFRHEQFAGYKADRPPTPDELLVQVPYVKRVLE
                     ALRVPTFEVPGWEADDLIGTLARRARDQGRHAVIVSSDKDLLQLVGEGISYYHPVRDA
                     FYDAADVERVFGVRPDQVPDVLALWGDLSDNIPGVPGIGDKGAKDLIRRFGSLEAVLE
                     RAAEVENKRYREPLLAHAAEARLSKELAIIRCDAPLEMAMEALALRPPDLETARALYT
                     ELEFGAFLRELPQAATVVEADTPLAVSDEQIARAVSGLTGTGPVGISLIRDHPDPMRA
                     GIVGLAFAAAEGGAAYVPVAHQGLGAPRGCPPSKLFEAVAPLFAADTGRLAGHDVKAD
                     LILMRRCGATAPRFGFDTMLASYLLNPERRGHDLEIVAREVAGMEVPIWSHLLGEGAR
                     AAPVADADAARVAATAGGRAAAVARLVPTLRRALEEDGLLPLLEDLELPLAGILADME
                     MAGVRIDVPFLAALSAEWDATLRRLTSEIHLLAGREFNINSPRQLGEILFETLKLAPG
                     RKTRKTGAWSTDSEVLEELAERHDLPRRLLEYRAIQKLKSTYVDALPALVHPQTGRVH
                     TSFNQAVAATGRLSSSDPNLQNIPIRTEQGRQIRRAFITDPGWTLLSADYSQIELRIL
                     AHLSGDPGLIEAFRSGDDVHRRTAAILFGVMPGLVTGDMRRRAKAVNFGIVYGMGPQR
                     LARDQGVSIRQAEEFITGYFAGFPRVKAYIDASIAAAEEEGKVRTLLGRVRYFPEIRG
                     ADRNARQQAIRAAVNSAIQGTAADIIKKAMVELHRRLARDRRRARMLLQVHDELVLEA
                     PDDEVEPVAALVREVMEGAQPLAVPLLAEVRRGPNWLDLKDAAGRSKE"
     gene            complement(3366..4652)
                     /gene="glyA"
                     /locus_tag="VFD06_02180"
     CDS             complement(3366..4652)
                     /gene="glyA"
                     /locus_tag="VFD06_02180"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZN02372.1"
                     /translation="MLAPLHEVDPVIAAALDAEVARQDETLVLIASENFVSRAVLEAM
                     ASVLTNKYAEGYPGRRYYGGCENADAVERLAIERALKVFGGEHANVQPHSGAQANMAV
                     FVALLKPGDTLLGMDLAHGGHLTHGHPLNFSGRYFRCVGYQVDRETERIDYEAVARLA
                     REHRPRLLIAGASAYARTIDFPRMRAIADDVGAVLMADAAHVAGLIAAGVHPNPVPHV
                     DVMTTTTHKTLRGPRGALIVCRERFAKAIDSAVFPGTQGGPLMNVIAAKAVALQEAMG
                     EPFKHDQRRTIENAATLAAGLQDGGLRIVSGGTDTHLFLVDLRPKGLTGKGVEQRLER
                     LGIALNKNAIPFDPEKPMIASGVRLGTPAVTSRGMGREEMHRIAALILEAIEGTEDPG
                     RDASIRARVAELTRAFPLYPWLRRPARTAGAAERSS"
BASE COUNT          659 a         1708 c         1721 g          722 t
ORIGIN      
        1 gatctcgagc gtcttgcgct gcaactcgga ggtgcgctcg aggacgcgcg cctcgaggcg
       61 ctcgtggtgc tcgcggttct cgcgcacgag gcgctgcgtc tccaggaccc gctcgacggt
      121 gaggcgcacc tgctccaggt ggaacggctt ggtgaggtag tcgctcgcgc cgagccgcat
      181 cgactgcagc gccgtgtcga agtcgtgcat cccggtgagc aggatcaccg cggtgtccgg
      241 cgccaccgcc ttgacctgct tcaggaggcc cacgccgtcg agccgcggca tgtccacgtc
      301 gctgatcagc aggcggcagg gagagcggcg caggtgctcg agggcggcgc atccgtcctc
      361 ggcggtgtcg cagcgatagc cgcactcggt gagcccctcg gacaggacct cgcggatggc
      421 gggatcgtcg tccacgacga ggatgttgat cggttcgtcc acgcgcgctc ccggcggtcg
      481 ccggggggag gtccgccgcg tccgctgcga tcaatctagg tcgcgcgggg gcgctgtcaa
      541 cgatgacatc gaagcgaacg gcgcgcggtg cgcggcctcg aggatgcgtc gcggcgcggc
      601 gccgatttca ttccttggag cggccggcgg cgtccttcag gtcgagccag ttcggcccgc
      661 ggcgcacctc cgccagaagc ggcaccgcga gcggctgcgc cccttccatc acctcgcgca
      721 cgagggccgc caccggctcc acctcgtcgt ccggcgcttc gagcaccagc tcgtcgtgca
      781 cctgcaggag catccgggcg cggcggcgat cccgggccag cctgcggtgc agctcgacca
      841 tcgccttctt gatgatgtcg gcggcggtcc cctggatggc gctgttcacc gcggcccgga
      901 tcgcctgctg gcgggcgttg cggtcggcgc cgcggatttc agggaagtaa cgcacccgcc
      961 ccaggagcgt gcgcaccttc ccctcctcct ccgcggccgc gatgctcgcg tcgatgtacg
     1021 ccttcaccct ggggaaaccg gcgaagtagc cggtgatgaa ctcttccgcc tgccggatgc
     1081 tcaccccctg gtcgcgggcc aggcgctgcg gtcccatgcc gtacacgatc ccgaaattca
     1141 ccgccttggc gcgccggcgc atgtcgccgg tgacgagacc cggcatgacg ccgaacagga
     1201 tcgccgcggt gcggcggtgg acgtcgtcgc cggaccggaa cgcctcgatg agccccggat
     1261 cgccgctcag gtgcgccagg atgcgtagct cgatctgcga gtagtcggcc gacaggagcg
     1321 tccagccggg atcggtgatg aaggcgcgcc ggatctgccg cccctgctcg gtgcggatcg
     1381 ggatgttttg caagttgggg tcgctgctgc tcagccggcc ggtcgccgcc accgcctggt
     1441 tgaaggaggt gtgcaccctg ccggtctgcg gatggacgag cgccggcagc gcgtcgacgt
     1501 aggtcgattt cagcttctgg atcgcgcggt actcgagcag gcgccgcggc agatcgtggc
     1561 gctccgccag ctcctcgagc acctcgctgt cggtcgacca cgcgcccgtc ttgcgggtct
     1621 tccggcccgg cgcgagcttg agcgtctcga acaggatctc cccgagctgc cgcggggagt
     1681 tgatgttgaa ttcgcgcccg gcgaggaggt ggatctcgga ggtcaggcgc cggagcgtcg
     1741 cgtcccattc cgccgacagc gcagcgagaa aggggacgtc gatgcggact ccggccatct
     1801 ccatgtcggc caggatcccg gcgagcggca gctccagatc ctcgaggagc ggcagcaggc
     1861 cgtcctcttc gagcgctcgc ctgagggtcg ggacgaggcg cgcgacggcg gcggccctcc
     1921 ccccggccgt ggcggcgacg cgcgcggcgt ccgcgtccgc gaccggggcg gcccgtgccc
     1981 cttcgccgag gagatggctc cagatcggca cctccatccc ggcgacctcg cgcgccacga
     2041 tttcgagatc gtgtccgcgc cgctcgggat tcagcagata cgaggcgagc atggtgtcga
     2101 acccgaagcg gggggcggtc gcgccgcagc ggcgcatcag gatcaggtcc gccttcacgt
     2161 cgtgccccgc cagccggccg gtgtccgcgg cgaacagcgg cgcgaccgcc tcgaagagct
     2221 tcgagggcgg gcacccccgc ggcgctccga gcccctggtg cgccaccggc acgtacgccg
     2281 ccccgccttc ggccgcggcg aaggccaggc cgacgatccc ggcccgcatc gggtccgggt
     2341 gatcgcggat gaggctgatc ccgaccggcc cggtccccgt caaccccgac accgcgcggg
     2401 cgatctgctc gtccgagacg gcgagggggg tgtcggcctc gaccaccgtc gccgcctgcg
     2461 gcagctcgcg caggaacgcc ccgaactcga gctcggtgta cagggcgcgg gccgtctcga
     2521 gatcgggcgg ccgcagcgcc agcgcctcca tcgccatctc gagcggcgcg tcgcaccgga
     2581 tgatcgccag ctccttgctc agccgcgcct ccgcggcgtg cgccaggagc ggctcccggt
     2641 acctcttgtt ctccacctcc gccgcccgct cgaggaccgc ctcgaggctc ccgaagcggc
     2701 ggatgaggtc cttcgccccc ttgtcgccga tccccggcac ccccgggatg ttgtcggaga
     2761 gatcccccca cagcgccagg acatccggca cctggtcggg gcgcacgccg aacacccgct
     2821 cgacgtcggc cgcgtcgtag aaggcgtcgc gcaccggatg gtaataggag atcccctccc
     2881 ccacgagctg cagcaggtcc ttgtcggaag agacgatcac ggcgtgccgc ccctggtcgc
     2941 gggcgcggcg cgccagcgta ccgatgagat cgtccgcctc ccagcccggc acctcaaagg
     3001 tcggaacccg gagcgcctcg agcacccgct tcacgtacgg cacctgcacc agcagctcgt
     3061 ccggggtcgg gggacggtcc gccttgtagc cggcgaactg ttcgtggcgg aaggtcggct
     3121 ccggcgtgtc gaacaggacg gcgagggcgt gcggctgaaa gtccttgagg agcttccgca
     3181 gcatgccggt gaagccgtag gtcgcgttgg tcgggaggcc gcggctgttc ctcagctcgc
     3241 ggatggcatg gaaggcgcgg tagagattgt tgctgccgtc gatgaggtag agagctcggg
     3301 aaggatcggg tcgtactccc atcgcgcgtc cgcggtcccc gaggctggcg cccgccgggg
     3361 cgtccctagg aggaccgctc cgccgcgccg gcggtgcggg ccgggcgacg gagccacggg
     3421 tagagcggga aggcgcgcgt cagctcggcg acgcgggcgc ggatcgaagc gtcgcgcccc
     3481 gggtcctcgg tcccctcgat ggcctcgagg atgagggcgg cgattcgatg catctcctcg
     3541 cgccccatcc cgcggctcgt gaccgcgggg gtccccagcc ggactcccga ggcgatcatc
     3601 ggcttctcgg gatcgaacgg gatggcgttc ttgttgagcg cgatcccgag gcgctcgagg
     3661 cgctgctcca cccccttgcc ggtcagcccc ttcgggcgca gatccaccag gaacaggtgc
     3721 gtgtcggtcc cgccggagac gatgcgcagc ccgccgtcct gcagccccgc cgccagcgtc
     3781 gcggcgttct cgatcgtgcg ccgctgatcg tgcttgaagg gctcccccat cgcctcctgg
     3841 agcgccaccg ccttggccgc gatgacgttc atcagcgggc cgccctgcgt gccggggaag
     3901 acggcggagt cgatcgcctt cgcgaagcgc tcgcggcaga cgatgagggc cccgcgcggt
     3961 ccgcgcagcg tcttgtgcgt ggtggtggtc atgacgtcga cgtgcggcac cgggttcggg
     4021 tgcacccccg cggcgatgag cccggcgacg tgcgccgcgt cggccatcag cacggcgccg
     4081 acatcgtcgg cgatcgcccg catgcgcggg aagtcgatgg tgcgggcgta ggcgctggcg
     4141 ccggcgatga ggagccgcgg ccggtgctcg cgcgccaggc gggccacggc ctcgtagtcg
     4201 atccgctccg tctcgcgatc gacctggtag ccgacgcagc ggaaataccg ccccgagaag
     4261 ttgagggggt ggccatgcgt caggtgcccg ccgtgcgcca ggtccatgcc gagcagcgtg
     4321 tcccccggct tgaggagcgc gacgaagacc gccatgttcg cctgcgcccc ggaatgcggc
     4381 tgcacgttgg cgtgctcccc gccgaacacc ttcagggcac gctcgatcgc cagccgctcc
     4441 accgcgtcgg cgttctcgca gccgccgtag tagcgccgcc ccgggtaccc ctcggcgtac
     4501 ttgttggtca gcaccgaggc catcgcctcg agcaccgcgc gcgagacaaa gttctcggaa
     4561 gcgatgagga cgagggtttc atcctggcgc gccacctccg cgtcgagcgc cgcggcaatc
     4621 acagggtcca cttcgtgcag gggggccagc aagaggtcgt cctccaggaa ggatgggatg
     4681 atatgacagc gatcgagacg gtcgcaaggt cggcaacgag gggtgccgtg gtcgaattcg
     4741 gctcccggca ctccggcttc gcccgacccg tcatggtacc aggcgcgtaa aagtaagcgt
     4801 ttattttcat
//
LOCUS       DASRIQ010000087         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3684472, whole genome shotgun sequence.
ACCESSION   DASRIQ010000087 DASRIQ010000000
VERSION     DASRIQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3684472"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3292
                     /locus_tag="VFD06_02185"
     CDS             <1..3292
                     /locus_tag="VFD06_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HZN02373.1"
                     /translation="WDGAPLGTAGLRRLATRIEARGGFPGPPGAHGPGDPENPVTMRA
                     YDPYGNLASETDPLGAVRRRDRGVQDPTFTFPETETDPLGHVTVRRFDPRTGLMTRMI
                     DPNGRAIEIEYDGFGRRLAEWGPGDTRERPTVSWRHETGTLPARVRRFARETSGQGER
                     TGTSGCLESVAWYDGLGRLLEVTAEHPDGGVVTGAVAFDAAGRVARRAEPFLSQAGVT
                     FVAPAPDAAAFAFEYDAAGRLARTTDPAGGVTLDAPGSVTAARIDPRGHRLETTLDAD
                     GRAVLVREFEGSAGSWTPRPPARYRYDPFGRLVGITDPMGAVTSLEYDLLGRRTRIDD
                     PHIGTWRSGYDRASRLIEEIDPQGRVTRLAYDAGGRLFEKALADGRRFQWRYDEGGAA
                     ASALGRLTSVSDPTGTQRFAYDALGRVVEATRTLFGSTFTARTEWDALGRIASRTLPG
                     AGRFEYGYDTGGRLRELHPYIDVIETDVRGQVSELHYRSGNRLTRVSEPATGRLRTIH
                     GVHRGGGTLLLLDHFYDADGLISEIDDLTVAATPVAHRYSYDARHRLLEASGPYGSLR
                     YDYDDGGTIRLKEGMTLSPGNPAQPQHLAFTSAGDAFDYDPAGHLVTRIRAGARQTLT
                     YDAGGRLVRLEEPARALVVTSDYDAGGHLVRETTDENGRRTTLLFPFPGVEVQDGTIT
                     ANIFLDTLRVLVIGPDGTAYHPMSDAVGSARIVVSEDGVVVARAAYRPFGDRHGTELS
                     DPISSLRYAGVHRQSASGLLTMGWRHYDPVYGRFLEPDHLIAAPLDPQSLNRYAYARD
                     NPVNLVDPNGHNPFLLLGILGAFALMDRDIRADAATSVALTAASIFLTGALGPGGAAG
                     VAALKASVPALYAAAATSILMHTPLGQGIADGYASLLQDLGLSPRASQAIARAGTGLL
                     LNSHLQRSFGTALSERGRLTRGDDLGTRADLDRALHERGLDPASFGTDSGDAYGTTVL
                     ASRDAAGNGSELQRFWELRDEAGNVPGVFGVRALGGGFEHGAAAIARTGANVLQTNHH
                     YFYLLGGISTQQVARDLFEAGYSASLFTLTGRASDFMIEFFYGPHGGGLVLGLDVAAS
                     GQNSGGDP"
     gene            complement(3321..>3542)
                     /locus_tag="VFD06_02190"
     CDS             complement(3321..>3542)
                     /locus_tag="VFD06_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02374.1"
                     /translation="TARMPPSPSGTTPTTVRFPGRPVQIARPSGSNSGCASAKALVAP
                     ATKARRASRPVPKGCGRPAGGAGRPRRLG"
BASE COUNT          505 a         1344 c         1210 g          483 t
ORIGIN      
        1 ctgggacggc gcgccgctcg gcacggccgg cctacggcgg ctcgcgaccc gcatcgaggc
       61 gcgcggaggc ttccccggcc cccccggggc gcacgggccc ggcgaccccg agaacccggt
      121 gacgatgcgc gcctacgatc cttacggcaa cctggcgagc gagaccgatc cgctcggcgc
      181 cgtgcgccgc cgggaccgcg gagtacagga cccgaccttc acgttccccg agaccgagac
      241 cgatccgctc ggtcacgtca ccgtgcggcg gttcgatccg cgcaccggcc tgatgacgag
      301 gatgatcgac ccgaacggcc gggcgatcga gatcgaatac gacggcttcg gccgccgtct
      361 ggcggaatgg ggtccgggcg acacgcgcga gcgcccgacc gtctcctggc gccacgagac
      421 cgggacgctg ccggcgcgcg tccgccgctt cgcgcgcgag acgagcggac agggggagcg
      481 gaccggcacg agcggctgcc tcgagtcggt cgcctggtac gacggcctcg gccggctgct
      541 ggaggtgacg gcggagcatc ccgatggagg cgtcgtcacc ggcgcggtgg cgttcgacgc
      601 cgcgggacgt gtggctcggc gcgccgagcc gtttctgagt caggccggcg tcacattcgt
      661 cgctccggcg ccggacgccg ccgcgttcgc tttcgaatac gacgcggccg ggcgcctcgc
      721 gcggacgacc gatccggccg gcggggtgac cctggacgcg ccgggctccg tgacggcggc
      781 ccggatcgat ccgcgcgggc accggctgga gacgacgctc gatgccgacg gccgggccgt
      841 tcttgtgcgg gagttcgaag gaagcgccgg ctcatggact ccgcgcccgc cggcacgcta
      901 ccggtacgac ccgttcggac gcctcgtcgg catcaccgat ccgatgggcg cggtcacgag
      961 cctcgagtac gacctcctcg ggcgccgcac gaggatcgac gaccctcaca tcgggacgtg
     1021 gcggtcgggc tacgaccggg cctcgcgcct gatcgaggag atcgacccgc agggtcgggt
     1081 gacgcgcctc gcctacgacg ccggcgggcg cctcttcgag aaggcgctcg ccgacggccg
     1141 gcgcttccag tggcgctacg acgaaggggg cgcggccgcc tccgcgctcg ggcggctgac
     1201 ctccgtctcc gatccgaccg gcacgcaacg cttcgcctac gacgcgctcg gccgggtcgt
     1261 cgaggcgacc cggaccctgt tcggatcgac cttcacggcg aggaccgaat gggatgcgct
     1321 cgggcgcatc gcctcgcgca cgctgccagg ggcgggccga ttcgagtacg ggtacgacac
     1381 gggaggacgc ctccgtgagc tgcatcccta catcgacgtc atcgaaacgg acgtccgcgg
     1441 ccaggtcagc gagctgcact accggtccgg aaaccgcctc acccgtgtca gcgagccggc
     1501 gacgggccgc ctccggacga ttcacggagt gcaccgcggc ggcggcacgc tcctgctgct
     1561 cgaccacttc tacgatgccg acggtctcat ctccgaaatc gacgacctga cggtcgccgc
     1621 gacacccgtc gcgcaccgct acagctacga cgcgcgccac cgcctcctcg aggccagcgg
     1681 tccctacgga tcgctgcggt acgactacga cgacggcggc accatccggc tcaaggaagg
     1741 gatgacgctg tctccgggga acccggcgca gccgcaacac ctcgcattca cttcggccgg
     1801 tgacgcgttc gattacgacc cggccggtca cctcgtcacc cgcatccggg ccggggcgag
     1861 acaaaccttg acgtacgacg ccggcgggcg cctcgtccgc ctcgaggagc cggctcgcgc
     1921 cctcgtcgtg accagcgact acgatgcggg cggacacctg gtacgcgaga cgaccgacga
     1981 gaacggccgg cgcacgaccc tcctgttccc cttccccgga gtcgaagtgc aggacggaac
     2041 gatcaccgcc aacatcttcc tcgacaccct cagggtcctc gtcatcggcc ccgacgggac
     2101 cgcgtatcac ccgatgagcg acgcggtcgg aagcgcccgg atcgtcgtga gcgaggatgg
     2161 ggtcgtggtg gcgcgcgccg cgtaccgccc cttcggcgat cgccacggaa cggagctctc
     2221 cgacccgatc tcctcgctgc gctacgcggg cgtgcaccgg cagtcggcca gcggcctcct
     2281 gacgatgggc tggcggcact acgatcccgt gtacggtcgc ttcctcgaac cggaccacct
     2341 cattgccgcg ccgctcgacc cgcaatcgct caaccgctac gcctacgcac gcgacaaccc
     2401 ggtcaacctc gtcgatccga acggccacaa ccccttcctg ctgctcggca tcctgggagc
     2461 gttcgccctc atggaccgcg acatccgcgc cgacgcggcc acctcggtgg ccctgaccgc
     2521 cgcgtcgatt ttcctgaccg gggcactcgg tccggggggc gcggccggag tcgcggcgct
     2581 caaggcctcg gtcccggcgc tctacgcggc ggcggccacc agcatcctga tgcacacgcc
     2641 gctggggcag gggatcgccg acggctacgc gagcctcctc caggatctcg gcctgtcacc
     2701 gcgagcctcg caggcaatcg cccgcgccgg caccggcctc ctgctcaatt cccatctcca
     2761 gagatccttc ggcaccgcgc tttcggagcg aggccggctg acgcgaggcg acgatctcgg
     2821 gacgcgcgcc gacctcgacc gggcgcttca cgagcggggc ctcgatccgg cgtcgttcgg
     2881 caccgactcc ggcgatgcgt acggaaccac ggtcctcgcc tcgcgcgacg cggccgggaa
     2941 cggctccgag ctccaacgct tctgggagct gcgcgacgag gccggcaacg tgccgggagt
     3001 gttcggcgtg cgcgcgctcg gcggcggctt cgagcacggc gccgcggcga tcgcccgaac
     3061 cggcgcgaac gtgctgcaga ccaaccatca ttacttctac ctactcggcg ggatctcgac
     3121 gcaacaggtg gcgcgcgatc tgttcgaggc ggggtattct gcgagcctgt tcacgctcac
     3181 cgggcgcgcg tccgatttca tgatcgagtt cttctacgga ccccacggcg gcggcctcgt
     3241 cctcggcctg gacgtggccg cgtcgggaca gaactccgga ggcgatccct gacgacaccc
     3301 acccgccgcc cccacggcgt ctacccgagg cgccgcggcc gtcccgcacc tcccgcggga
     3361 cggccgcacc ctttcgggac cggtctgctg gccctcctcg ccttcgtcgc cggcgcgacg
     3421 agcgccttcg cggatgcgca cccgctgttc gagccggacg gccgggcgat ctgcaccggg
     3481 cgtcccggga accgcaccgt cgtcggcgtc gtccccgacg gcgacggggg cattctcgcg
     3541 gt
//
LOCUS       DASRIQ010000088         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_35040, whole genome shotgun sequence.
ACCESSION   DASRIQ010000088 DASRIQ010000000
VERSION     DASRIQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35040"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFD06_02195"
     CDS             complement(<1..323)
                     /locus_tag="VFD06_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase B"
                     /protein_id="HZN02375.1"
                     /translation="MGRPLSLLLLAAAALLALAGCASAQRQKYAFDPPFSVGDPQFRR
                     SLDNIGGVLVGGNSATLLENGDGVFPAMLKDIREARASVNLETYIYKPDEVGRQFADA
                     MIEAAG"
     gene            complement(1463..>2383)
                     /locus_tag="VFD06_02200"
     CDS             complement(1463..>2383)
                     /locus_tag="VFD06_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02376.1"
                     /translation="LRLTSNSGFSSNPAIAWSESEYGIAWSDNTLGRGQISFARVGCE
                     GEGDCVEPSPEICDGVDNDCNGLVDDGLGPGTLSVGLEPPRLWPPNHRMVAIHATVTL
                     SGGCPAACPAPPVVILASVSSDEPDDANGGGDGNTLNDIQDAAVGSADFDLELRAERD
                     SDGAGRHYEVAYVATDCFGSAAAASATILVPHDENGAVEPVRISVTNSADGTLLAWDP
                     VPGAVSYRVVRGNTASLRDAGEFIELGEVTCIRADSTVTATQGDEDAENPPLGQAFFY
                     LVAYSDQWGGSGYGTAAVPKPRAAASGDCP"
BASE COUNT          378 a          832 c          793 g          380 t
ORIGIN      
        1 ccggccgctt cgatcatcgc gtcggcgaac tgccttccga cctcgtcggg cttgtagatg
       61 taggtctcca gattcaccga ggccctggcc tctcgaatgt ccttgagcat cgcggggaag
      121 acgccgtcgc cgttttcgag cagcgtggcg gagttcccgc ccacgaggac gccgccgatg
      181 ttgtcgagcg atcggcggaa ctgggggtcc ccgaccgaga acggcggatc gaacgcgtac
      241 ttctgccgct gagccgaggc gcagccggcc agcgcgagga gcgccgcggc ggcgaggagg
      301 agcaaggaga gcggccgtcc catcgtccag caccccccaa gcctcggtga gccggttcgt
      361 cgtgccaagc caacgttcca ggccactcta gcactggcgt ggggacttgc cagaaaactc
      421 gactcaggac gtcacccgaa gcgatcgagt tcgaatcgcc gcgcgggtaa atcgtccgcg
      481 tcctgcgact cattcgtgat ctccacggga taacttgacg gacgctcatc gcacttctag
      541 gtttgaggac gggaagagtc acggccgaac tgggggagga tcgcaatcat gcaggcagac
      601 tctccgcgtt gcacgcggtc gattcacacg gcgctcctca ccatcgcgat ctccgcctgt
      661 tccggctcgc cgcagacggg gacggcatca cgtcatccag cgtctgctcc cgcgccgccg
      721 gggacggcca gcgagatctc cgccggcatc accacgcgcc ccgaatccga gggcgcgccc
      781 acgtcgaacc catccgggcc ttcttcggag gccgccgccg ggcctccgcc tccctcgacg
      841 agggagatcc atggctactt caccaatacg ccctcccgta actcgctgcg cctttggaag
      901 ggcccgaacg atcacaaggg cttcgagcgt tggacccggt tcaccgtcga ggtcgacggc
      961 gtgccggaat actcacgggc cgtcagcacc atccggctcg tcggcgtctg ccgctcgtcc
     1021 ggcaagggac tcgatcagct gttgctctct ttttatcccg aacgtcgcgg tggcggggcg
     1081 ttcggggtcc tccggtacga tacgaaggaa cagcggttca cgctcggctt cgccccaaag
     1141 gatgtgcgcg tcgactgcgc gggcggtcgc gccatcttcc ccgaaggagc gccggcgctc
     1201 ccctgcacct gcccgtggcg ggatggcccc gacgatctct cgggggcagc ggccgcgacg
     1261 gccgacgcga gggaaatcct gattctgccg gaggaggccg gagggccggc gttcacgcga
     1321 ggagaaccgc ccaccggggc cgacgcgcca gcgcgggccg cggggcccgg cgggcaatga
     1381 ggggaatacg acgcgaaacg ctgcggcggc cggctccagg gacaggatct tccgaggcgt
     1441 cgcacaccgt cggaattcgt tcttaaggac agtcccccga tgccgcggct cgcggcttgg
     1501 gcacggccgc ggtcccatag ccgctgccgc cccactggtc gctgtaggcc acgaggtaga
     1561 agaacgcctg tccgagcggc gggttctcgg catcttcgtc cccctgtgtt gccgtgaccg
     1621 tcgagtccgc ccggatgcag gtcacctcgc ccagctcgat gaactcgccg gcgtcccgca
     1681 ggctggccgt gttgccgcgc accacacggt aggaaaccgc cccgggaacg gggtcccaag
     1741 cgagaagtgt tccgtcggcg gaattcgtga cggagatccg gacgggttcg acggcgccgt
     1801 tctcatcgtg gggcacgagg atcgtcgcgc ttgcggcggc ggcgctcccg aagcagtccg
     1861 tggccacgta cgcaacctcg taatgtcggc cggcgccatc ggaatcgcgt tccgcccgca
     1921 actccagatc gaaatccgcg ctgccgaccg ccgcatcctg gatgtcgttc agggtgttgc
     1981 catctccgcc cccgttcgcg tcgtccggct catcgctcga aaccgaggcc aggatgacga
     2041 cggggggagc cgggcaggcc gcagggcatc cgccgctcag ggtgaccgtc gcgtggatgg
     2101 cgaccatgcg gtgattcggc ggccagagcc gcggcggctc gagcccgacg ctcaacgtgc
     2161 ccggtccgag cccatcgtcg accaggccgt tgcagtcgtt gtccacaccg tcgcagatct
     2221 cgggtgacgg ctccacgcag tcgccttcgc cctcgcagcc gaccctcgcg aaggagatct
     2281 gccctcgccc cagggtgttg tcggaccagg cgatcccgta ctcgctctcc gaccaggcga
     2341 ttgccggatt ggacgaaaac ccgctgttgc tggtcaggcg cag
//
LOCUS       DASRIQ010000089         6112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_290597, whole genome shotgun sequence.
ACCESSION   DASRIQ010000089 DASRIQ010000000
VERSION     DASRIQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290597"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VFD06_02205"
     CDS             complement(<1..907)
                     /locus_tag="VFD06_02205"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HZN02377.1"
                     /translation="MLALLLAASPVLAIAETVPDRSAGLGERSDRNLFGPLRIRDMTP
                     FNLLRLDMLPAHAVEAEPHSFAIEADVSVANTFAMSSNVRRYLQQRGERTSLTAADAE
                     AILGLGQDAFYMDGEFGLLELRFHYRVLSRTSVSLGVSAYDFSGGFMDGSIEGFHDGF
                     GLRADGRDLVARRQFQTVMSLDGAGYAALETPVEQGMGDPVLGVRHSVPLGGSRWTLV
                     MSGEAKIAWQGERPFLSTGTNDYGVQLALQRKFSRQAVYCNASLVSTDGQVFGIDLER
                     RVIPTFTAAYEVEMTEFTNLIGQVYA"
     gene            1215..1568
                     /locus_tag="VFD06_02210"
     CDS             1215..1568
                     /locus_tag="VFD06_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02378.1"
                     /translation="MDKLIYLDSGYDWSAPEFFKPFEQMLVTVAPVEAELASLDAYRA
                     WFRTAWLGKAAWSDGLEGDLRAIAQPDAGGRLHPVPTEKVFAANRDRKVAARLQEGAR
                     PGAGALRADLLPRAW"
     gene            1588..1767
                     /locus_tag="VFD06_02215"
     CDS             1588..1767
                     /locus_tag="VFD06_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02379.1"
                     /translation="MRAFERKIMAPFRRASIERLRREKRGGVKVKELAGRTHMSIGVE
                     KPDELASIVRDFLAP"
     gene            complement(1843..2010)
                     /locus_tag="VFD06_02220"
     CDS             complement(1843..2010)
                     /locus_tag="VFD06_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02380.1"
                     /translation="MEPLDDPKRKSPQQKDDPNQTDDQRDVKRGDDREETEEQEEIEG
                     PDEEESGDDEA"
     gene            2213..3790
                     /locus_tag="VFD06_02225"
     CDS             2213..3790
                     /locus_tag="VFD06_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HZN02381.1"
                     /translation="MARSRFRRRIRSTLAALACALLFSPGDIVLLAQQDKPPAPPAGE
                     DKPAKPPEQPEKPPEQPAKPEQPPKETPAGEKPATPPDTTKKEPGAEQAPKITNDQLD
                     SLVAPIALYPDALLAQVLVASTYPLEIVQLHQWLAKNKTLKDKALADAVAKQPWDPSI
                     QSMAALPDLVKRLSDDVQWTTDLGNAFLAQQSDVMDAVQRMRKKAQEKGALKSNEQQK
                     VSEEKAGEPPKTVIVVESANPEVVYVPSYSPTVVYGAPIYPYPPIYYPPYPPGAAFMS
                     FSIGVMWGAAIWGGSCCGCGWGGGDVDINVNNNFNRNEINTGGRNQVNTGDRGGAGTG
                     NRGGAGASAGTSDRGAGGPGGGAGAGNKAGGGKWQHNPSHRGGTPYSDKATANKYGGN
                     ARGDSLSQRQSSAGNRAGGGPAAGARPSTGMGGTGAGPGGSAGRPGGGSGAGPSAGTR
                     DMGGGAGGGDRIGSRDIPSSGSRGGSGGGRSGYSGGGLGSGGGYSGGSARSSSSRGSR
                     SMGGRSFGGGGGGGRRR"
     gene            3795..4769
                     /locus_tag="VFD06_02230"
     CDS             3795..4769
                     /locus_tag="VFD06_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HZN02382.1"
                     /translation="MSRSSRILLLSVLALAVVLTAAPVASAQTSEAPATPSVPKPPAT
                     PQQTFPTPEAAADALIAAATVFDVEALKKILGSEGLDLVVTADTVQDENAAKAFAEQA
                     QIRRSVVIDSTNPKIATLNVGAEDWPTPVPMVRTPSGQWRFDTKAGRVEVLRRRIGRN
                     ELDAIQVCRGYVEAQQEYAFERRDGKVNQYAQRIISTPGKKDGLVWKTADGTWEGPIA
                     EPIATAIAEGYSSRFEPYHGYYFKVLKGQGPAAQLGRIDFVVNGAMIGGFALAAAPAE
                     YGNTGVMTFIVSHDGVVFEKDLGPTTLETFKSMTLFNPDKTWKPVEEP"
     gene            5001..>6112
                     /locus_tag="VFD06_02235"
     CDS             5001..>6112
                     /locus_tag="VFD06_02235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011497516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HZN02383.1"
                     /translation="MKRSRSAVFSLFVLAAFLTVVTGGPLSTPGGGRPPAGTPAFVED
                     EVLVKFHPSAQAADKAAARAEVQGSKVRSFKSGAEHWKVGRGLGAVQAIERLMRNPRV
                     VYAEPNYILTADVVPNDPRFNELYGMLNTGQTGGTTDADIDADQAWGVSRGSHDVLVG
                     VIDTGIDYNHPDLAANIWTNPGEIPGNTIDDDGNGFVDDIHGYDFANNDSDPFDDNGH
                     GSHCSGTIGGVGNNGVGVAGVNWQVSLMALKFLSGGGSGTTANAVRAVEYSTMMGVDL
                     TSNSWGGGGFSQALYDAVAAAGAANIAFVAAAGNDGTDNDVSPHYPSNYDLANVISVA
                     ATDHNDAKASFSNWGATTVDLGAPGVNILSTLPGNS"
BASE COUNT         1028 a         2078 c         2132 g          874 t
ORIGIN      
        1 tggcgtaaac ctgcccgatc aggttcgtga actccgtcat ctccacctca taggcagccg
       61 tgaaggtcgg gatgacgcgg cgctcgaggt cgatgccgaa cacctggccg tcggtgctca
      121 cgaggctggc gttgcagtag accgcctgcc ggctgaactt ccgctgcaga gcgagctgca
      181 cgccgtagtc gttggtcccc gtcgagagga acggccgctc cccctgccag gcgatcttcg
      241 cctcgccgct catgacgagc gtccagcgcg atccgccgag cgggacgctg tggcgcacgc
      301 cgaggaccgg atctcccatg ccctgctcca ccggcgtctc gagcgccgca tacccggccc
      361 cgtcgagcga catgacggtc tggaactgcc ggcgcgccac gaggtcgcgc ccgtcggcgc
      421 ggaggccgaa gccgtcgtgg aatccttcga tgctgccgtc catgaacccg ccggaaaagt
      481 cgtaggcgga gacgcccagg gacacggacg tccggctcag gacgcgatag tggaagcgca
      541 gctccaacag gccgaattcg ccgtccatgt agaaggcgtc ctgtcccagc ccgaggatcg
      601 cctcggcgtc ggccgccgtc agcgaggtgc gctcgccgcg ctgctgcaga tagcggcgca
      661 cattgctgct catcgcgaag gtgttggcga ccgacacgtc ggcttcgatc gcgaagctgt
      721 ggggttcggc ctcgaccgcg tgcgccggca gcatgtcgag ccggagcagg ttgaacggcg
      781 tcatgtcccg gatgcgcagc ggaccgaaca gattcctgtc ggagcgctcc ccgaggccgg
      841 cggagcgatc ggggacagtc tctgcgatcg ccaggactgg gctcgccgcc agcaggagcg
      901 cgagcacgat gccttcgagt actgtccgat gcgaatttcc ggacatcctg tatctccgag
      961 gagacaagca gcgtgccacg gacggagatt cccggtcaac agaaatgcgg cgggagtcgc
     1021 gttcctcccg ccggggatgt cggtctggtc agggagagga agggggcccg aggcgttcgt
     1081 gggcgacccg cccttcatct gcgaggggcc aatcttgggc aaaaggtact caagtaaaat
     1141 ggcgggcccg tgacaatcct ggtgcgtctg ggtggcaggt acggactgtg gcccttggcg
     1201 agcatccgga gcgcgtggac aagctcatct acctggactc gggctacgac tggtccgccc
     1261 cggagttctt caagccgttc gagcagatgc tcgtgacggt cgctccggtc gaggccgagc
     1321 tcgcgagcct cgacgcctat cgcgcctggt tccggacggc gtggctcggg aaggcggcgt
     1381 ggagcgacgg gctcgagggg gaccttcgcg ccatcgcgca accggacgcc ggcgggcgcc
     1441 tgcacccggt gcccaccgag aaggtgttcg ccgccaaccg cgatcgaaag gtggccgcgc
     1501 gactacagga aggtgcgcgc cccggcgctg gcgctctacg cgccgatctc cttccccgag
     1561 catggtgatg ccgcgcgcct ggagaaggtg agggccttcg agcggaagat catggcgccg
     1621 ttccggcgtg ccagcatcga gcgcctccgg cgcgaaaagc gggggggcgt gaaggtgaag
     1681 gagctcgccg ggcgcaccca catgtcgatc ggcgtggaga agcccgacga gctggcgtcg
     1741 atcgtgcgcg acttcctggc accctgacgc acgtcggccg ccggatgtct ccgaaccctc
     1801 ccaggtgaag aagacggccg gcggccgctc ttgtgtcaac ggctaagctt cgtcgtcgcc
     1861 gctttcttcc tcgtccggcc cttcgatctc ctcctgctcc tcggtctcct cgcggtcgtc
     1921 cccgcgcttc acgtcgcgct ggtcgtccgt ctggttcgga tcgtccttct gctgcggcga
     1981 ctttctcttc ggatcgtcca gcggttccat gggcatcctc ctcttgaact tcgggcacga
     2041 agggtacggt gcgccaaccc ggctccccat tggtgaagtt accgagaggc gttcggaatt
     2101 ggcctgatcg agcactggcc gggcaccgcg gtccctcccc cattgccgtc aagcagttag
     2161 gcattggttg cgatatgctc cgcccgtctc caacaggtcg ggaggcccca cgatggctcg
     2221 gagcaggttc cggcggcgga ttcggtcgac tctggcggcg ctcgcgtgcg cgctgctctt
     2281 ctccccgggg gatatcgtcc tcctggcgca acaggacaag cctccggcgc cgccggccgg
     2341 ggaggacaag ccggcgaaac cgccggagca gccggagaag ccgccggagc agccggccaa
     2401 gcccgagcag cctcccaagg aaacccctgc cggtgagaag ccggccacgc cccccgacac
     2461 caccaagaag gagccggggg cggagcaggc gccgaagatc acgaacgacc agctcgactc
     2521 gctggtcgcg ccgatcgcgc tctatcccga cgcgctgctg gcgcaggtcc tggtggcctc
     2581 gacctatccg ctcgagatcg tgcagctcca ccagtggctg gcgaagaaca agacgctcaa
     2641 ggacaaggcg ctcgccgacg ccgtcgccaa gcagccgtgg gacccgagca tccagtcgat
     2701 ggcggcgctg ccggacctcg tgaagcgcct ctccgacgac gtccagtgga ccaccgacct
     2761 cggcaacgcg ttcctggcgc agcagagcga cgtcatggac gccgtgcagc gcatgcgcaa
     2821 gaaggcgcag gagaaaggcg ccctgaagtc gaacgagcag cagaaggtga gcgaggagaa
     2881 ggcgggggag ccccccaaga cggtcatcgt cgtcgagtcg gcgaaccccg aggtcgtcta
     2941 cgtgccgtcg tatagcccga ccgtggtgta cggcgcgccg atctatccct atccgccgat
     3001 ctactacccg ccgtacccgc ctggcgcggc gttcatgtcg ttctccatcg gggtgatgtg
     3061 gggggcggcg atctggggcg gctcgtgctg cggctgcggc tggggcggcg gcgacgtcga
     3121 catcaacgtc aacaacaact tcaaccgcaa cgagatcaac accggcggcc ggaaccaggt
     3181 caacaccggc gatcggggcg gcgctggcac cgggaaccgc ggcggggcgg gggccagcgc
     3241 ggggaccagc gatcgcggag cgggcggccc cggaggtggc gctggcgccg gcaacaaggc
     3301 gggcggcggc aagtggcagc acaacccgtc ccaccgcggc ggcacgccgt actcggacaa
     3361 ggccaccgcc aacaagtacg gcggcaacgc ccgcggcgat tccctgagcc agcgccagtc
     3421 gagcgccggg aaccgagccg gcggcggtcc ggccgcaggc gcccgtccca gcaccggcat
     3481 gggcgggacc ggggccggtc cgggagggag cgccggtcgt cctggaggcg gctccggcgc
     3541 cgggccgagc gccggcacgc gcgacatggg gggtggcgcg ggcggcggcg atcgcatcgg
     3601 cagccgcgac atcccttcga gcggcagccg cggcgggagc ggcggcggtc gcagcggcta
     3661 cagcggcggg gggctcggca gcggcggggg ctacagcggc gggagcgccc ggtccagcag
     3721 cagccgcggt tcacgcagca tgggcgggcg ctcgttcggc ggcggaggcg gcggtggccg
     3781 caggagatag cgcgatgagc cgttcgagcc gcatcctcct cctgagtgtc ctggccctgg
     3841 cggtcgtcct gacggcggcg cctgtggcga gcgcccagac gtcggaggcc ccggcgacgc
     3901 cgtctgtccc gaagcctccg gcgacgccgc agcagacctt cccgacgccg gaggcggcgg
     3961 ccgacgcgct catcgccgcg gccacggtgt tcgatgtcga ggcgctgaag aagattctcg
     4021 ggtcggaagg cctcgacctc gtcgtcaccg ccgacacggt gcaggacgaa aacgccgcga
     4081 aggcgttcgc cgagcaggcg cagatccgac gctcggtcgt catcgattcg accaacccga
     4141 agatcgccac cctcaacgtc ggcgcggagg actggccgac tcccgtgccc atggtgcgga
     4201 cgccgagcgg gcagtggcgc ttcgacacga aggcgggccg cgtggaggtc ctgcggcgcc
     4261 gcatcggccg caacgagctg gacgccatcc aggtttgccg cggttacgtc gaggcgcagc
     4321 aggaatacgc cttcgaaagg cgcgacggga aggtcaacca gtacgcccag cgcatcatca
     4381 gcacgccggg gaagaaggat ggcctggtgt ggaagacggc cgacggcacg tgggaagggc
     4441 cgatcgccga accgatcgcc acggcgatcg ccgaggggta ctcgagccgc ttcgagccgt
     4501 accacggcta ctacttcaag gtgctgaagg ggcagggtcc cgccgcacag ctcggtcgga
     4561 tcgacttcgt cgtgaacggg gcgatgatcg gcggcttcgc gctcgccgcc gccccggcgg
     4621 agtacggcaa caccggcgtg atgacgttca tcgtcagcca cgacggcgtc gtcttcgaga
     4681 aggacctcgg cccgacgacg ctcgagacct tcaagtcgat gaccctcttc aatcccgaca
     4741 agacctggaa gccggtggaa gagccgtaat cggacgtcag aagagcgcgc caccctgaca
     4801 tcgaatgtgt cgccgcagca tcgctcgccg ccctccttat gcgttaatcg cagaaaaggg
     4861 ccgggcgtcc cgcatttctg aatctcccct attgacacag gaattccggc ggcgcaaaag
     4921 agtcgctgaa actcgctcac catcctggtt ggccgaaaca cagaagtcgg gcgcgcccgc
     4981 gcgccccgtg gagggatcga atgaagcggt cgcgctccgc cgtgttctcc ctgttcgtcc
     5041 tcgccgcctt cctgaccgtc gtcaccggcg gacctctgtc cacgccgggc ggcggccgcc
     5101 cgcctgcggg cacgccggcg ttcgtcgagg acgaagtcct ggtcaagttc cacccctcgg
     5161 cgcaggcggc cgacaaggcg gccgccaggg ccgaggtcca gggctcgaaa gtgcgttcgt
     5221 tcaagagcgg cgccgagcac tggaaggtgg ggcgcgggct gggcgcggtg caggcgatcg
     5281 agagactcat gcggaatcct cgggtggttt acgccgagcc gaactacatc ctcacggccg
     5341 acgtcgtgcc gaacgacccg cgcttcaacg agctgtacgg gatgctcaac accgggcaga
     5401 ccggcggcac cacggacgcc gacatcgacg ccgaccaggc ctggggtgtg tcgcgtggca
     5461 gccatgacgt tctcgtgggc gtcatcgata ccggcatcga ctacaaccat cccgacctcg
     5521 cggccaacat ctggacgaac cccggcgaga tccccggcaa caccatcgac gacgacggca
     5581 acggtttcgt ggacgatatc cacggttacg acttcgccaa caacgactcc gacccgttcg
     5641 atgacaacgg ccacgggagc cactgctccg ggaccatcgg cggcgtcggc aacaacggcg
     5701 tcggggtggc cggagtcaac tggcaggtga gcctcatggc gctcaagttc ctgagcggcg
     5761 gcggctcggg gaccaccgcg aacgcggtgc gggcggtcga gtactcgacc atgatggggg
     5821 tcgatctcac cagcaactcg tggggcggcg gcggcttctc gcaggccctc tacgacgccg
     5881 ttgcggcggc gggggcggcc aacatcgcct tcgtggcggc ggccggcaac gacggcaccg
     5941 acaacgacgt ctcgccgcac tacccgtcga actacgacct ggcgaacgtc atcagcgtcg
     6001 ccgcgaccga tcacaacgat gccaaggcgt cgttctccaa ctggggcgcg acgaccgtcg
     6061 acctgggcgc gccgggagtc aacatcctta gcacgctgcc cggcaactcc ta
//
LOCUS       DASRIQ010000090         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3157918, whole genome shotgun sequence.
ACCESSION   DASRIQ010000090 DASRIQ010000000
VERSION     DASRIQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3157918"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1011)
                     /locus_tag="VFD06_02240"
     CDS             complement(265..1011)
                     /locus_tag="VFD06_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZN02384.1"
                     /translation="MISVVIPTLDEESALPATLAALRDQEDAPPFEVILADGGSRDTT
                     VALFESVPAVGASADDVAGAEAGPGGLHRRVVLCAARGRAAQMNAGAALARGDALLFL
                     HADTLLPPRGLAAIDAALRDPRVAGGGFRMRYLEPDARLRIVAAWATLRSRLTGIHYG
                     DQGMFLRRDLFRRLGGFPPLPLFEDLRLARLLRKEGTVITLSYAVRTSGRRLLRDGVA
                     RTSLQFGWLKLRHALGADPARLWREYRDVR"
     gene            1058..>2590
                     /locus_tag="VFD06_02245"
     CDS             1058..>2590
                     /locus_tag="VFD06_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZN02385.1"
                     /translation="MSPFSPESTSSTSASPSTDATAPRGAGSGGGFTTGPPSGGSSSG
                     GAAPPGPPSGGPGSGGRFSWLTRRWKLWGGILLGAMLVATIAGATLGWFLTSDIPEVK
                     SLQDWKPPVVTTLYSADGKPLYQFGAEKRIVVDLDQVPQAFLDGLVATEDSHFYDHVG
                     VDPWGIARAIVKDIIHMRKAQGGSTLTQQLARSLFLKPEKTWGRKIQEMVLALQIEET
                     FTKGEILTFYVNQVYMGHGRYGVEAAARFYFGKPGKELTLPEAALLAGLVQRPEAYSP
                     VRAPERATARRNHVLRRMVEEKKLEREAADAASKEPVAIAKVQDEDNLAPYFVEEVRR
                     YLDATYGEVALYQEGLEVYTTLDPALQRAANQAVFNGLRALDKRRGWRGAPRNLIKEK
                     EDPARFTHATWMRPPVVGRLRWGVVTEVGKKDAAVRLGEWTARVDAAGVRWTGRGLAQ
                     VLKVGDVAPFLVQKIDETKKTLAVALDQEPDAESALLVLDPETGEVRALVGGWDFNRS
                     QFD"
BASE COUNT          399 a          918 c          923 g          350 t
ORIGIN      
        1 ccatgcccga tgcacggaac gaagcagtac cccacgacgc ccagcggcgc ctgcctgcca
       61 ggctggagga gcacccggag cgtcgcggcc ggaaccagca tgccgccgcg gccaagagtc
      121 agcccgcggg ggtacgcctg cccccatgcg gtcggcagtc ttgccgtcga cgtcgcatcg
      181 gccgtctccg tttgggtctg ggaggccgtc ccgctggtca ccggcgaagt ctagcatcgc
      241 gcaaagcccg gctgacaggg gctctcagcg gacatcccgg tattcccgcc acaggcgtgc
      301 cggatcggcc ccgagcgcat ggcgcagctt cagccagccg aactgcaggg acgttctggc
      361 caccccgtcc ctgaggagcc ggcggcccga ggtacggacg gcgtacgaaa gcgtgatcac
      421 ggttccttcc ttgcgcaaga gccgcgcgag acgcagatct tcgaagagcg gcagcggcgg
      481 aaaccctccc agacgccgga acaggtcgcg ccggaggaac atcccctgat cgccataatg
      541 gattccggtc aggcgcgatc gcagcgtcgc ccaggcggcg acgatgcgca gccgcgcgtc
      601 cggctcgagg taccgcatcc ggaagccgcc gcctgcgacg cgcgggtccc ggagcgcggc
      661 gtcgatcgcc gcgagtcctc gtgggggcag cagcgtatcg gcgtgcagga acagcagtgc
      721 gtcgccccgc gcgagggccg cgccggcgtt catctgggcg gcccggccgc gtgccgcaca
      781 gagcaccacg cgccggtgga ggccgccggg acctgcttcg gcaccggcca cgtcgtcggc
      841 cgatgcgccc actgcgggga ccgattcgaa cagcgcgacg gtcgtgtcgc ggctgccgcc
      901 gtcggcgagg atcacctcga acggcggcgc atcctcctga tcgcgcagcg ccgcgagcgt
      961 cgcggggagg gccgactcct cgtcgagcgt tgggatgacg accgagatca tctatactcc
     1021 cccgcccggc gccggcgggg tcgcgccgtg aggtgacgtg agccccttct caccggaatc
     1081 cacttcctcc acatcggcat ccccttcgac cgatgcgacc gctccgcgcg gcgccgggtc
     1141 gggcggcggg ttcacgaccg ggccgccttc gggcggctcg tcgagcggcg gcgccgcccc
     1201 gccgggtcct ccttccggcg gccccggcag cggcggccgt ttttcgtggc tgacgcgccg
     1261 ctggaagctc tggggcggca tcctcctcgg cgcgatgctc gtcgcgacca tcgccggcgc
     1321 cacgctcgga tggttcctca cctccgacat tcccgaggtg aagtccctgc aggactggaa
     1381 gccgccggtc gtcaccaccc tctacagcgc cgacggcaag cctctctacc agttcggcgc
     1441 cgagaagcgg atcgtcgtcg atctcgacca ggtgccgcaa gcgttcctgg acggactggt
     1501 cgcgaccgag gactcgcact tctacgatca cgtcggcgtc gatccctggg ggatcgcgcg
     1561 cgccatcgtc aaggacatca tccacatgcg caaggcgcag ggggggagca ccctgacgca
     1621 gcagctggcg cgctccctct tcctcaagcc ggagaagacc tggggacgca agatccagga
     1681 gatggtgctg gcgctgcaga tcgaggagac gttcaccaag ggcgagatcc tcaccttcta
     1741 cgtcaaccag gtgtacatgg ggcacggccg ctacggcgtc gaagcggcgg cccgcttcta
     1801 tttcggcaag ccggggaagg agctgacgct gccggaggcg gcgctgctcg ccggactcgt
     1861 gcagcgcccg gaggcgtact cgccggtgcg ggcgccggag cgggcgaccg cgcggcgcaa
     1921 ccacgtcctg cgtcgcatgg tcgaggagaa gaagctggag cgcgaggcgg cggatgccgc
     1981 ctcgaaggag ccggtggcga tcgccaaggt ccaggacgag gacaacctcg cgccgtactt
     2041 cgtcgaggag gtgcgccgct acctcgacgc cacctacggc gaggtcgcgc tctaccagga
     2101 agggctcgag gtgtacacca cgctcgatcc ggccctgcag cgggccgcca accaggcggt
     2161 gttcaacggc ctgcgcgccc tcgacaagcg ccgcggctgg cgtggcgcac cgcgcaatct
     2221 gatcaaggag aaggaggacc cggcccgctt cacgcacgcc acctggatgc ggcctcccgt
     2281 ggtcggccgg ctgcgctggg gggtggtcac cgaggtcggc aagaaggacg cggcggtccg
     2341 cctcggcgag tggaccgcgc gcgtcgatgc cgccggggtg aggtggaccg gccggggcct
     2401 cgcgcaggtt ctcaaggtcg gcgacgtcgc gcccttcctg gtgcagaaga tcgacgagac
     2461 gaagaagacc ctcgccgtgg cgctcgacca ggagccggac gccgagtcgg cgctcctggt
     2521 gctcgatccc gaaaccggcg aggtgcgggc gctggtcggc ggctgggact tcaaccgcag
     2581 ccagttcgac
//
LOCUS       DASRIQ010000091         4871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3158078, whole genome shotgun sequence.
ACCESSION   DASRIQ010000091 DASRIQ010000000
VERSION     DASRIQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3158078"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFD06_02250"
     CDS             <1..730
                     /locus_tag="VFD06_02250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZN02386.1"
                     /translation="AGGGFLLAFAGASKLYPGLLGVYLLTRRRWRDIAWTVACGVGFA
                     LLSLLAFGRGPWDAFLEHMPRLLSGESFPAFRNPMAMAINFSVPGLVFKAHVFGAPAS
                     FEAMKIAGWVYTFAAVAAIWFVARRPLRPEEKPVLWLAMLILATLRSPFLPQSYAGFP
                     ALWLLTLGGAIEEPTPRRLAIILTIWAGLNIMWPLDWPIDPRALAILSSVPQALTILL
                     AVLALVPRRAPATDEDVAPMRLAA"
     gene            753..887
                     /locus_tag="VFD06_02255"
     CDS             753..887
                     /locus_tag="VFD06_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02387.1"
                     /translation="MRLAAVVLGAILCGIDGLMMTGPFGCIAGGVAGWWVSGRRPPPA
                     "
     gene            916..1653
                     /locus_tag="VFD06_02260"
     CDS             916..1653
                     /locus_tag="VFD06_02260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZN02388.1"
                     /translation="MPGAPSLLFLAYLLGVLPWAAIRSARRVEQALGRSGRSPASDRL
                     AIWGSTLVAQGLLLVLAWLAGRGFGFRFFAVALTPAGLVAAAAALGACFVLRAVARAL
                     RTEEERKALVVYRLAPRSAVEWLAWGAVALVASVTEEIAYRGVGVAILSHWSGSLAAA
                     ILVCATAFAVAHALQGWKSGVVIFMLALIFHALVLFTGTLVLAMLVHLLYDVIAAVAI
                     AREAERDDRAAPPPGQAPVSPEDGMTC"
     gene            complement(1650..3326)
                     /locus_tag="VFD06_02265"
     CDS             complement(1650..3326)
                     /locus_tag="VFD06_02265"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZN02389.1"
                     /translation="MTTGPAADPIPRPSLRLTAGMALAGLALVAAGRATPAESDPGAA
                     IRPEAIRAHMRFLSDGALEGRASDGRGYAIAASYVAAQMEALGLEPAGGPGSFLQKVP
                     MRKAILDEAATALVLVRDGKEEPLALPDDFFAAPDRQHLKADVEAPVVFVGFGITAPE
                     LGHDDYRGVDARGKIVATVWGGPKRFEATERAHFSNNTVKQENAAAHGAVGYLAIMTP
                     DDLERYRWEWLLPQSRAGSMAWLDERGIPNDTFPGIRAVARLNGKGAARLLRGAPKSP
                     EEIYAAARDGVPPVFDLPVKARLRVAARHTGFESSNVLGLLRGSDPRLKDEQVVLSAH
                     LDHLGLCPPVEGDEVCHGAYDNASGVATLLEIARAFTAMRPAPRRSILFAIVTGEEKG
                     LLGSDYLARHPAVPGTVVANVNMDGAVGILYPPADIVALGIEHSTLKRPVQDAARLLG
                     YTLSPDPMPEESIFVRSDQYSFVRQGVPSVFIVDGGKSTNPGVDGLKLIRTWLTTLYH
                     TPADNMSQPFDFTSAAKGARLNFLVTRNVAQAEARPAWNEGDFFGGKFGR"
     gene            complement(3323..3784)
                     /locus_tag="VFD06_02270"
     CDS             complement(3323..3784)
                     /locus_tag="VFD06_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN02390.1"
                     /translation="MTAQPMQRITVPEARAAMERDPKTIYLDVRTEEEFADGHPARAL
                     NIPVGHANPVTRGFELNQDFATVAKAVLPLDTPLLVGCRTGPRAEAAAQILASEGYSN
                     VTWVFGGYAGITGPAGNRIAPGWLESGFPVTREAEPGEAYEDLKKKAGVGR"
     gene            3931..>4871
                     /locus_tag="VFD06_02275"
     CDS             3931..>4871
                     /locus_tag="VFD06_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HZN02391.1"
                     /translation="MGDEAIDPVCGMRTDPTHPRGGRFEHGGRTFSFCSEGCRSRFAA
                     DPEGFLSGTRTPGHGPESAVDAVEYTCPMHPEIRQRGPGACPICGMALEPVRVTLEAG
                     PDAEERDMTRRLVAAAILTVPVFLLAMVAMPHGASEGAVAHGGGAPWTHWLQAALSLP
                     VVVWCGAPFFVRGWQGVVRRSPNMFTLIALGTGAACAFSLVATLVPHRLPAAFRRADG
                     SVDVYFESAAVIVTLVLLGQVLELRARRATGGAVRALLALASRTARRVRPDGGEEDVP
                     LAAIRAGETIRVRPGERVPVDGTVISGHGVVDESMLTG"
BASE COUNT          582 a         1681 c         1829 g          779 t
ORIGIN      
        1 cgccggggga gggttcctcc tggcgttcgc cggggcgagc aagctttatc cgggtcttct
       61 cggcgtgtac cttctgaccc gccggcgctg gcgcgacatc gcctggacgg tcgcctgcgg
      121 cgtcgggttc gcgcttctga gtcttctggc cttcggtcgc gggccttggg acgccttcct
      181 ggagcacatg ccgcgtctgc tgagcggcga atcgttcccg gcctttcgca acccgatggc
      241 gatggcgatc aacttctccg tccccggtct cgtcttcaag gcgcacgtct tcggagcgcc
      301 cgcatcgttc gaggcgatga agatcgcggg atgggtctac acgttcgcgg cggtcgcggc
      361 gatctggttc gtggcgcggc gtccgctgcg cccggaggag aagccggtcc tgtggctggc
      421 gatgctgatc ctcgcgacgc tcaggagccc gttcctgccg cagtcgtatg ccggctttcc
      481 cgccctctgg cttctgaccc tcggaggggc gatcgaagag ccgaccccgc ggcgcctcgc
      541 gatcatcctc acgatctggg ccgggctgaa catcatgtgg ccgctcgact ggccgatcga
      601 tccacgcgcg ctggcgatcc tgtccagcgt gccgcaggct ctgacgatcc tcctcgcggt
      661 cctcgccctg gtgccgcggc gcgcaccggc gacggatgag gacgtcgcgc cgatgcgtct
      721 ggcggcgtga tgggcatcct ggagagggga gcatgaggct tgcggccgtc gtcctcggag
      781 cgatcctctg cggcatcgat gggctgatga tgaccgggcc gttcgggtgc atcgcgggcg
      841 gggtggccgg gtggtgggtc tccggacgcc ggcctccgcc ggcatgagcg gtggcgtccc
      901 gcgaggtgac gcctgatgcc cggcgcgccg tcgctgctct tcctcgcata tctgctgggg
      961 gtcctgccct gggcggcgat ccggagcgca cgccgcgtcg aacaggcgct ggggcgatcc
     1021 gggcgcagcc ccgcatccga ccgcctggcg atctggggga gcaccctcgt cgcgcagggg
     1081 cttctcctgg tcctcgcctg gctcgcggga agggggttcg gattccggtt cttcgccgtg
     1141 gcgctgacgc cggccggcct cgtcgccgcg gccgcggcgc tcggcgcgtg cttcgtgctg
     1201 cgcgcggtcg cccgcgctct tcgcaccgag gaggagcgca aggcactcgt ggtgtaccgc
     1261 ctggcgccgc gcagcgcggt cgagtggctg gcgtggggtg ccgtcgccct ggtcgcgagc
     1321 gtgaccgagg agatcgccta ccgcggggtc ggagtggcga ttctgagcca ctggagcggg
     1381 agtctcgccg ccgcgatcct cgtctgcgcc acggcgttcg cggtggcgca cgcgctgcag
     1441 ggctggaaga gcggcgtcgt gatcttcatg ctggcgctca tcttccacgc gctcgtgctg
     1501 ttcaccggca cgctcgtcct ggcgatgctg gtgcacctgc tctacgacgt catcgcggcg
     1561 gtggcgatcg cccgggaggc ggagcgggac gatcgcgccg ccccgcctcc cggacaggcg
     1621 ccggtgtcgc ccgaggacgg catgacctgc tagcggccga acttcccgcc gaagaagtcc
     1681 ccttcgttcc aggcggggcg ggcctcggcc tgcgcgacgt tgcgcgtgac gaggaagttg
     1741 agtcgcgccc ccttcgccgc cgacgtgaag tcgaacggct gcgacatgtt gtcggccggc
     1801 gtgtgataga gggtcgtgag ccaggtgcgg atgagcttga gtccgtccac cccggggttc
     1861 gtgctcttcc cgccgtcgac gatgaagacc gacggcaccc cctggcgcac gaacgagtac
     1921 tggtcgctgc ggacgaagat cgactcctcc ggcatcggat cggggctgag cgtgtagccg
     1981 aggaggcgcg ccgcgtcctg caccgggcgc ttgagggtcg agtgctcgat gccgagcgcc
     2041 acgatgtcgg cgggcggata gaggatgccg accgccccgt ccatgttgac gttggcgacg
     2101 accgtgccgg ggaccgccgg gtggcgcgcc aggtagtcgg atccgaggag ccccttctcc
     2161 tccccggtga cgatggcgaa caggatcgat cggcgcggcg cggggcgcat cgccgtgaag
     2221 gcgcgggcga tctcgagcag ggtcgccacg cccgacgcgt tgtcgtaggc gccgtgacac
     2281 acctcgtccc cctcgaccgg aggacagagg ccgaggtgat cgaggtgggc gctcaggacc
     2341 acctgctcgt ccttgagacg cgggtcgctg ccgcgcagca gaccgaggac gttggagctc
     2401 tcgaatcccg tgtgccgggc cgcgacccgg agacgcgcct tcaccgggag atcgaagacc
     2461 ggcggcacgc catcccgggc ggcggcgtag atctcctccg gactcttcgg cgcgccgcgc
     2521 agcaggcgcg ccgccccctt gccgttgagc cgcgcgacgg cccggatccc ggggaacgtg
     2581 tcgttcggga tgccgcgctc gtcgagccag gccatcgatc cggcccgcga ctgcggcagg
     2641 agccactccc agcggtagcg ctccagatcg tcgggcgtca tgatggcgag gtaccccacg
     2701 gcgccgtgcg cggcggcgtt ctcctgcttg acggtgttgt tggagaagtg ggcccgctcg
     2761 gtcgcctcga agcgcttcgg tcccccccac acggtcgcga cgatcttgcc gcgcgcgtcg
     2821 accccgcggt aatcgtcgtg cccgagctcc ggggcggtga tgccgaagcc gacgaacacc
     2881 accggcgcct cgacgtcggc cttcaggtgc tgccggtccg gcgccgcgaa gaagtcgtcc
     2941 ggcagggcga gcggctcctc cttgccgtcc cgcacgagga cgagcgcggt cgcggcctcg
     3001 tcgaggatcg ccttgcgcat cggcactttc tggaggaacg agccggggcc gccggccggt
     3061 tcgaggccga gcgcttccat ctgcgccgcc acgtaggagg cggcgatggc gtagccgcgg
     3121 ccgtcggagg cgcgcccttc gagcgcgccg tccgacagga agcgcatgtg ggcgcggatc
     3181 gcctccggcc ggatcgccgc cccgggatcc gactccgccg gtgtggcgcg cccggccgca
     3241 acgagtgcga gaccggcgag ggccatgccg gccgtgagac gcagcgaggg gcgggggatg
     3301 gggtccgcgg ccgggccggt cgtcatcgcc cgaccccggc tttcttcttc aggtcttcgt
     3361 aggcctcccc cggctccgcc tcccgggtga cgggaaatcc ggactcgagc cagccggggg
     3421 cgatccggtt cccggcgggg ccggtgatgc cggcgtagcc tccgaacacc caggtgacgt
     3481 tggagtaccc ctcgctcgcg aggatctgcg ccgcggcctc ggcgcggggc cccgtgcggc
     3541 agccgacgag caacggcgtg tcgagcggca ggaccgcctt cgcgacggtc gcgaaatcct
     3601 ggttcagctc gaatccccgc gtcaccggat tggcgtggcc gaccgggatg ttgagcgccc
     3661 gcgcgggatg accatcggcg aactcctcct cggtgcggac gtccaggtag atcgtcttcg
     3721 gatcgcgctc catcgcggcg cgcgcctccg ggacggtgat gcgctgcatc ggctgcgccg
     3781 tcatcggctc cccctgtggc ggtggtccag atccatggaa ctccgggagc agtgacgata
     3841 gcgggcgcgg ccgggggagg tcaaccgtgc cggcgaacgt cgcgaccgcg gcagggctct
     3901 gccttgacga gtgtggtatg ggatcggggc atgggtgatg aggcgatcga cccggtctgc
     3961 ggcatgcgca ccgaccccac gcatccgcgc ggaggccgct tcgagcacgg cgggcggacg
     4021 ttctctttct gctcggaggg atgccggtcc cgcttcgccg ccgacccgga gggcttcctg
     4081 tccggaacgc ggacgccggg gcacgggccg gaatccgcgg tcgatgccgt cgaatacacc
     4141 tgtccgatgc acccggagat acgccagcgc ggtccgggcg cctgtccgat ctgcggcatg
     4201 gcgctcgagc ctgtgcgggt cacgctcgaa gcgggccccg atgcggaaga gcgcgacatg
     4261 acgcgccgcc tcgtcgccgc ggcgatcctc accgtaccgg tgttcctcct cgccatggtg
     4321 gccatgccgc acggcgcgtc ggaaggcgcc gtggcgcatg gcggaggggc tccatggacc
     4381 cactggctgc aggcggcgct gagtcttccg gtcgtcgtgt ggtgcggggc gccgttcttc
     4441 gtgcggggct ggcagggggt cgtgcgccgg agccccaaca tgttcacgct catcgcgctg
     4501 gggaccgggg cggcgtgcgc cttcagtctc gtcgcgaccc tcgtgccgca tcgtctgccg
     4561 gcggcgttcc gccgcgccga cggctcggtg gacgtctact tcgagtcggc ggcggtgatc
     4621 gtgaccctcg tgctcctcgg ccaggtgctg gagctgcggg cgcggcgggc gaccggcggg
     4681 gcggtgcgcg cgctgctggc cctggcctcg aggacggcgc gccgcgtgcg cccggacggt
     4741 ggcgaagagg acgtgccgct cgcggcgatc cgcgccggcg agacgattcg cgtgcgcccc
     4801 ggcgagcggg tgccagtgga cgggacggtc atctccggcc acggcgtcgt ggacgagtcg
     4861 atgctcaccg g
//
LOCUS       DASRIQ010000092         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1594369, whole genome shotgun sequence.
ACCESSION   DASRIQ010000092 DASRIQ010000000
VERSION     DASRIQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594369"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..956)
                     /gene="lsrF"
                     /locus_tag="VFD06_02280"
     CDS             complement(66..956)
                     /gene="lsrF"
                     /locus_tag="VFD06_02280"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HZN02392.1"
                     /translation="MPEADHGKEAKEFHLDIPAENRPFFLKGSGALDWGTQNRLSRIF
                     DPESGRTVMLAIDHGYFQGPTTGLERVDVSIVPLLPYCDALMVTRGILRTTIPPTFRK
                     GIVLRASGGPSLLKELSDEEIAVDADEICRLHVAALAVQIFVGGPQETRSIHNMTRLV
                     DLGNRLGVPVFAVTAVGTEMTRDARYFRLACRIAAELGAHFVKTYYVPEGFETVTASC
                     PVPIVMAGGKKQPEIDALTMAHRAIQEGAAGVDMGRNIFQSDAPAAMLQAVRKVVHEN
                     VKPREALDLYESLRAGRKGR"
     gene            complement(1070..2116)
                     /gene="cydB"
                     /locus_tag="VFD06_02285"
     CDS             complement(1070..2116)
                     /gene="cydB"
                     /locus_tag="VFD06_02285"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZN02393.1"
                     /translation="MVELWFGIVSLTVTLYVLLDGYDLGAGALHLLVAKRDSERREVL
                     ASIGPYWDGNEVWLIATGGLVYVAFPKVLGSALSGFYLAMMLVLWTLILRGLAIEFRS
                     HLKAPLWRQAGDVVFSVASALLVIVLGAAIGNLVRGMPIDRDGWFALALFTDFGASGE
                     VGILDWYTVLSGLFTLVALSAHGAAYLCHKTSGPVQERARRLGRPLAAAVGMMWLAVT
                     LATWRVSPDFLATIPKRPLAWGSALLALGGLVLLNLGLRSGRDGRAFLGSNLFLGGLL
                     AATAAGLYPVLLRSIGDPTLSLTAHGTATAASSLRIAFAWWAIAFPLAILYYVIVARL
                     HRGKVQVPAEGEGY"
     gene            complement(2109..>3158)
                     /locus_tag="VFD06_02290"
     CDS             complement(2109..>3158)
                     /locus_tag="VFD06_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZN02394.1"
                     /translation="QTLAMEGMFAFFLESSFLAILVWGEKRVGRVGHFLASLALFAGA
                     WISGYFIICTNSFMQHPVGHAVGADGRLHLVDFSAFLFNPWAIAAYLHTMLGAVVTGS
                     FVVTAVGAFYLLTGQFRDAAVRFTQIGVIAGFVASLLVAFPTGDHQAKLVAKHQPAAL
                     AAMEGRFESGPMAEITVIGQPDVAAKKMDNPIAVPGILSFLAHGAFTAEVHGLDEFPQ
                     EDWPDNIELLYYVFHIMVGLGTIFIVLTGIAALLLWRGHLFRNRAALWILMIAAPFPY
                     IANTAGWMSAELGRQPWLVYGLFRTKEGASPTVHAGSTIFTLIGFCGLYLVIGLAFLF
                     LVGREIAHGPGSRHG"
BASE COUNT          555 a         1077 c         1094 g          432 t
ORIGIN      
        1 caccggcggc tgcgtgaggt gcagcgcgtc gcgcagcggt tcggcgtgcg cggcccagtc
       61 gttcgtcatc gtccctttct cccggcgcgg agcgactcgt acaggtcgag ggcttcgcgc
      121 ggcttcacgt tctcgtgcac cacctttcgc accgcctgca gcatcgccgc cggcgcgtcc
      181 gactggaaga tgttgcgccc catgtccacg ccggccgccc cctcctggat ggcgcggtgc
      241 gccatggtga gcgcgtcgat ctccggctgt ttcttgccgc cggccatgac gatcggcacc
      301 gggcacgagg cggtcaccgt ctcgaaccct tccggcacgt agtaggtctt gacgaagtgc
      361 gcccccagct ccgccgcgat gcggcaggcg agccggaagt agcgcgcgtc gcgcgtcatc
      421 tccgtgccga ccgcggtgac ggcgaacacc ggcaccccga gccggttccc gagatcgacc
      481 aggcgcgtca tgttgtggat ggagcgggtc tcctgaggcc cgccgacgaa gatctgcacc
      541 gcgagcgctg ccacgtgcag acggcagatc tcgtcggcgt ccaccgcgat ctcctcgtcc
      601 gacagctcct tgaggaggct cggcccgccg ctggcgcgca ggacgatccc cttgcggaag
      661 gtcggcggga tcgtcgtcct caggatgccg cgcgtcacca tcagcgcgtc gcagtacggc
      721 aggagcggta cgatcgacac gtccacgcgc tcgagacccg tggtcggccc ctggaagtag
      781 ccgtgatcga tcgccagcat caccgtccgg ccgctctccg gatcgaagat gcgcgacagc
      841 cggttctgcg tcccccagtc gagcgcgccg cttcccttca ggaagaaggg gcgattctcg
      901 gccgggatgt cgagatggaa ctccttcgcc tctttgccgt gatcggcttc gggcatgtcg
      961 tcgatcctct cctggacgtg cgctgcacgc ccgcttgtat cagaagaaac cgggtccctt
     1021 caaccgcggg acgagacgga ccctgacctt ccgacaaggc cggcgggggt cagtacccct
     1081 ccccctcggc cggcacctgc accttgccgc gatgcaggcg cgccacgatg acgtaataaa
     1141 ggatggcgag gggaaaggcg atcgcccacc aggcgaaggc gatccgcagg ctcgatgccg
     1201 cggtggcggt gccgtgggcg gtcagcgaca gggtcgggtc gccgatcgag cgcagcagga
     1261 ccggatagag ccccgccgcc gtggcggcga ggagcccgcc gaggaagagg ttcgatccga
     1321 ggaacgcccg gccatcgcga cccgacctca aaccgagatt gagcagcacg agcccgccga
     1381 gtgccaggag cgccgacccc caggcgagcg gccgcttcgg gatggtcgcg aggaaatcgg
     1441 ggctcacgcg ccaggtcgcg agggtgaccg cgagccacat catcccgacc gcggccgcca
     1501 gcggccggcc gagacggcgc gcccgctcct gcacggggcc ggacgtcttg tggcagaggt
     1561 acgccgcgcc gtgggccgag agggcgacga gcgtgaacag tcccgacagc accgtgtacc
     1621 agtcgaggat gccgacctcc ccggaagcgc cgaagtcggt gaacagggcc agcgcgaacc
     1681 agccgtcgcg atcgatcggc atcccgcgca cgaggttgcc gatcgccgcc ccgaggacga
     1741 tgacgaggag cgcgctcgcg acgctgaaga cgacgtctcc cgcctggcgc cacagggggg
     1801 ccttcaggtg gctgcggaac tcgatggcga ggccgcgcag gatgagggtc cacaggacca
     1861 gcatcatcgc gagatagaag ccggagagag ccgaacccag caccttggga aaggcgacgt
     1921 agacgaggcc gccggtggcg atgagccaga cctcgttgcc gtcccagtag gggccgatgc
     1981 tcgccagcac ctcacggcgc tcgctgtcgc gcttcgccac cagcaggtgc agcgccccgg
     2041 cccccagatc gtacccgtcg agcagcacgt agagggtgac ggtcagggag acgatcccga
     2101 accacagctc aaccatgccg ggacccgggg ccgtgggcga tctcgcggcc gacgaggaag
     2161 aggaacgcga gaccgatgac gagatacagc ccgcagaagc cgatgagggt gaagatcgtc
     2221 gagccggcgt gcaccgtcgg gctcgccccc tccttcgtcc ggaagaggcc gtacaccagc
     2281 cacggctgcc gccccaactc ggcgctcatc cagccggcgg tgttggcgat gtaggggaac
     2341 ggggcggcga tcatcaggat ccagagtgcc gcgcggttgc ggaagaggtg cccgcgccac
     2401 aggaggagcg ccgcgatgcc ggtcagcacg atgaagatcg tcccgagccc gaccatgatg
     2461 tggaagacgt agtacaggag ttcgatgttg tccggccagt cctcctgcgg gaactcgtcg
     2521 aggccgtgca cctcggcggt gaacgcgccg tgcgcgagga acgagaggat cccgggcacg
     2581 gcgatcgggt tgtccatctt cttcgcggcg acgtccggct ggccgatgac ggtgatctcg
     2641 gccatcggtc cgctttcgaa ccgcccttcc atggcggcga gcgcggccgg ctggtgcttc
     2701 gccacgagct tcgcctggtg atcgccggtc gggaaggcga ccagcaggct cgcgacgaac
     2761 ccggcgatca cgccaatctg cgtaaaccgt accgccgcgt cgcggaactg cccggtcagg
     2821 agatagaacg ctccgaccgc cgtgaccacg aacgatccag tgaccaccgc cccgagcatc
     2881 gtgtgcaggt aggcggcgat cgcccacgga ttgaagagga aggcggagaa atcgaccagg
     2941 tgcaggcggc cgtccgcgcc gacggcgtgg ccgaccgggt gctgcatgaa ggagttggtg
     3001 cagataatga agtagccgga gatccaggcg ccggcgaaaa gcgccagcga cgcgaggaaa
     3061 tgccccacgc ggccgacccg tttctccccc cagaccagga tcgccaggaa cgacgactcg
     3121 aggaagaaag cgaacatccc ctccatcgcc agcgtctg
//
LOCUS       DASRIQ010000093         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1342789, whole genome shotgun sequence.
ACCESSION   DASRIQ010000093 DASRIQ010000000
VERSION     DASRIQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342789"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VFD06_02295"
     CDS             complement(<1..841)
                     /locus_tag="VFD06_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02395.1"
                     /translation="MTRLQRRLRPGLPIAVLLLLPGAFLWGGRLQQRSSQDQDDPAGQ
                     AMEVWMARIAERLRGPETYSPPASFSPFGGPIEIRRLPAEAGRDLLLTSQGFVEWDAA
                     RDAVAARVPAALRLEPGTFHRSARGTLRAGLNYLRLSESAVAARGLDAVQAEVMEHAR
                     ILGWLPERTLLVHVEPREVEALARAAAIDRSRALEPYQKVAPSLGLLPRLSRQEAENP
                     DLLARLMFVPGLGGPAAIAAIERMPGVSEVIPDATQEDGAQLRIRHDRVADLARRDEV
                     LAIE"
     gene            complement(1023..2498)
                     /locus_tag="VFD06_02300"
     CDS             complement(1023..2498)
                     /locus_tag="VFD06_02300"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZN02396.1"
                     /translation="MIGAGAETLMGLLPVLVPAVGGLWILVQVAILKQRTLRWCAGQT
                     LLVLGFAAASDVLLWRSGAEGTMLDGALLLDPQLLAFHVLFVVVAALTVLVSMAHLEA
                     LEWPHGEFYALLLFAVSGLTIMAGTENLLSLFIGLEVLSIPLYILAGFTRDRVYAIEA
                     ALKYFLLGAFSTGIFLYGIALLYGASGSVTLRGVADALQASRGGPIDPILLAGGALLL
                     IGLAFKVAAVPFHFWAPDVYQGALAPVAGFMAAGTKAAAFAAFVRVVSVALNGPPAKE
                     QWIAVTSVVAVLTMILGNLVALAQQNIKRMLAYSSIANAGYLLVALAAVGKMNAEASS
                     IIFYLFVYAFMTIGAFAVAAILGRDGEADHAYSLTAYAGLAKRRPMLAAAMTVFMLSL
                     TGIPPAAGFMAKFYIFRDAVRADLVGLAAVGLLTSVIGAFYYLRVVVQMYFRDPAPEP
                     AGGPGLRIAATDALAIGAAAIAVLAIGLFPTALLDLVGFSG"
     gene            complement(2495..>3323)
                     /locus_tag="VFD06_02305"
     CDS             complement(2495..>3323)
                     /locus_tag="VFD06_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZN02397.1"
                     /translation="LSGEQDIVKMGGLRKHLPWTHATFLIGTLAIAGVPPLAGFFSKD
                     EILWAAIAGRGGSLLLWFAGVVTAGLTAFYMTRLYMLTFAGPSRLTHEAEHHLHESPA
                     VMLLPLILLAVGSVAAGFVGVPHFLGGEAIPNFFEHWLAPVMPAVAAAEGSAPLSEWA
                     AMFVALLASGAGIAAGYAMYRGGPRPERLTPAVRWVRAKYYVDELYDLSVIRPYRALC
                     RFSSAWDARIVDGAVNAVGFVTDLSGEMLRLTQTGYVRNYALGFFGGTILILYYVLR"
BASE COUNT          576 a         1170 c         1179 g          398 t
ORIGIN      
        1 gctcaatcgc cagcacctcg tcgcgccggg ccaggtcggc gacccggtcg tggcggatcc
       61 gcagctgcgc gccgtcctcc tgcgtcgcat cgggaatcac ttcgctgacg ccgggcatcc
      121 gctcgatcgc ggcgatcgcc gcgggcccgc cgagaccggg aacgaacatc agccgcgcca
      181 ggagatccgg gttctccgcc tcctgccggc tgaggcgcgg caggagcccc agcgacgggg
      241 cgaccttctg gtagggctcc agggcgcggc tccgatcgat cgccgcggcg cgcgccagcg
      301 cttcgacctc gcgcggctcg acgtggacga ggagggtcct ctccggaagc cagccgagga
      361 tgcgggcgtg ctccatcacc tcggcctgca ccgcgtccag tccgcgggcc gcgacggcgc
      421 tctccgacag gcgcaggtag ttgagaccgg cgcgcagcgt tccgcgcgca gagcggtgga
      481 acgtcccggg ctcgaggcgc agcgcggccg gcacgcgggc ggcgacggcg tcgcgcgccg
      541 cgtcccattc gacgaatccc tggctggtca ggagaaggtc gcgccccgcc tccgcgggga
      601 ggcgccggat ctcgatcggg ccgccgaacg gtgagaacga ggccggcggc gaatacgtct
      661 cggggccgcg caggcgctcc gcgatccgcg ccatccagac ctccatcgcc tgcccggccg
      721 gatcgtcctg atcctggctc gaacgctgct ggaggcgtcc tccccacagg aaggctccgg
      781 ggagcaggag caggacggcg atcggcaaac ccggacgcag gcggcgctgc aggcgcgtca
      841 tcggaccctc ccggcgccgg aaaggatccc tccccgatcc ctgggaaggc gagtgcggca
      901 cttctttccg gcgcgttcag agcgagttgt taaaacagca tataccgcga tggcttttcg
      961 gcgtcaacct tcttggctgc tgagggatgc gtcggagcgg gggggtggaa ggttgcgcgg
     1021 tttcagcctg agaaaccgac gagatccaga agcgcggtcg ggaacaggcc gatcgccagg
     1081 acggcgatgg cggcggcgcc gatcgccagg gcgtcggtgg cggcgatccg gagcccgggg
     1141 ccgccggccg gctcgggggc cgggtcccgg aagtacatct gcaccacaac cctcaaatag
     1201 taaaaggcgc cgatgaccga ggtgaggaga ccgaccgcgg cgaggccgac gagatcggcc
     1261 cgcaccgcgt cccggaagat gtagaacttc gccatgaagc cggcggcggg cgggatgccg
     1321 gtgagcgaca gcatgaagac ggtcatcgcc gcggcgagca tcggccgccg cttggcgagc
     1381 ccggcgtagg cggtcaggga gtaggcgtgg tccgcctccc cgtcccggcc gaggatcgcc
     1441 gccaccgcga aggcgccgat ggtcatgaag gcatagacga agaggtagaa gatgatcgac
     1501 gaggcctcgg cgttcatctt gccgacggcc gccagcgcca ccagcaggta gccggcgttg
     1561 gcgatgctgg agtaggcgag catcctcttg atgttctgct gcgccagggc gacgaggttg
     1621 ccgaggatca tcgtcaggac cgccacgacc gaggtgaccg cgatccactg ctccttggcg
     1681 ggcgggccgt tgagcgccac cgacacgaca cgcacgaagg cggcgaacgc cgccgccttg
     1741 gtgccggccg ccatgaaacc ggccaccggc gccagcgccc cctggtacac gtccggcgcc
     1801 cagaagtgga acggcaccgc ggcgaccttg aaggccagcc cgatgagcag aagcgccccg
     1861 cccgcgagca ggatcggatc gatcggcccg ccgcgcgacg cctgcagggc gtcggccacg
     1921 ccgcgcagcg tgacgctgcc gctggcgccg tacaggagcg cgatgccgta caggaagatc
     1981 ccggtcgaga aggcgccgag caggaagtac ttgagcgccg cctcgatggc gtagacgcga
     2041 tcgcgcgtga agccggccag gatgtagagc gggatcgaca gcacctcgag cccgatgaac
     2101 aggctcagga gattctcggt gccggccatg atggtcaggc cggagacggc gaagagcagc
     2161 agcgcgtaga actcgccgtg cggccactcg agcgcctcga gatgcgccat cgagaccagc
     2221 acggtcagcg ccgccaccac gacgaacagg acgtggaagg cgaggagctg cgggtcgagg
     2281 agcagcgccc cgtcgagcat cgtcccctcc gcccccgacc gccacaggag gacgtcggac
     2341 gccgccgcga agcccagcac caggagcgtc tgcccggcgc accagcgcag cgtccgctgc
     2401 ttcaggatcg ccacctgcac caggatccag agcccgccga ccgccggaac caggaccggc
     2461 agcagcccca tgagcgtctc cgcgcccgcg ccgatcaccg cagcacgtaa tagaggatca
     2521 ggatcgtccc cccgaagaag ccgagcgcgt agttgcgcac gtagccggtc tgcgtcagcc
     2581 gcagcatctc gccgctcagg tcggtgacga acccgacggc gttcaccgcg ccgtccacga
     2641 tgcgcgcatc ccacgcggac gagaagcggc acagggcgcg atacggccgg atgacagaaa
     2701 gatcgtagag ctcgtccacg tagtacttgg cgcgcaccca gcgcaccgcc ggcgtgagcc
     2761 gctccgggcg cggtccgccg cggtacatcg cgtaaccggc agcgatgccg gcacccgagg
     2821 cgaggagcgc gacgaacatc gccgcccatt ccgacagcgg cgcacttccc tccgcggcgg
     2881 ccacggccgg catcaccggc gcgagccagt gctcgaagaa gttcgggatc gcctcgccgc
     2941 cgaggaaatg cggcaccccc acgaacccgg cggccaccga cccgaccgcc aggaggatca
     3001 gcggcagcag catgaccgcc ggcgattcgt gcaggtggtg ctccgcctcg tgcgtcagcc
     3061 gggacggccc tgcgaaggtc agcatgtaga ggcgggtcat gtagaaggcg gtcagccccg
     3121 ccgtcaccac cccggcgaac cagaggagga gactcccgcc gcgtccggcg atcgccgccc
     3181 agaggatctc gtccttggag aagaagccgg cgaggggcgg cacgccggcg atcgccagcg
     3241 tcccgatcag gaaggtcgcg tgcgtccacg gcaggtgctt gcgcagccct cccatcttca
     3301 ctatgtcttg ctcccccgac agc
//
LOCUS       DASRIQ010000094         5509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_292502, whole genome shotgun sequence.
ACCESSION   DASRIQ010000094 DASRIQ010000000
VERSION     DASRIQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292502"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..934)
                     /locus_tag="VFD06_02310"
     CDS             complement(380..934)
                     /locus_tag="VFD06_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HZN02398.1"
                     /translation="MKKLVSLAFLGVLVFSAPALAGIEKGNGEIGVDYGSTSYDNDAA
                     EFDSSDSLSLRGGYFFTNLFQLEGQYISSDTTVEEIGESIKASTDIMMVNGVFNFMPG
                     KEIKPYVLVGLGQASTDIDAEGFGSVDDSGTAYQIGAGSRFFFGKSKRAAFRFDVSMI
                     NQDTFEEETTNTTVAGGFTFRLGK"
     gene            1114..2871
                     /locus_tag="VFD06_02315"
     CDS             1114..2871
                     /locus_tag="VFD06_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HZN02399.1"
                     /translation="MPHPYRSRRLLRILAVVAVLLVLAALAARPSLERTLRSRLEAEG
                     ARRGLEVRVERVRLGLWPPLRLHRIAVEKAGRMRLEAEDLDVWWRGRPRVEIVNAVLR
                     GPAGLAIHAEWTVWDVLAADRDHLRVALARPANGLVLDRTTAPGRTTWTVQATDLATG
                     RLLEVRRRDRLILDGGTLRGSLQCLEEAGEVRFDLDVGAQSARLPALAPAAAQPDQDP
                     FDEREMGEPVQIGLQATGAWNRADGVLDIPHYGATLAGASASGTLSLRAIGRDPAIDL
                     SLEVEKIDFAQLLRASGLPPPEGMAAPGAAASADPSTGAPDVGLGSASLFATAQGRLS
                     DPASFVVTQNLRFTPPARLPRAIERLRGDFVHEVGLRSGARRGIEVSSASPDFIPLQD
                     VPPLFRRALLLAEDAGFYSHPGVDLREVPAALLTDLARGGAVRGASTITQQLAKNLFL
                     SRDKNVGRKLQELSLALLLESSLGKDRILEIYLNVIEWGPDLFGLRPAARAYFGREPA
                     ELTPAQTAFLVSLIPAPVKYQVSFKDGTPGPGLRQLMDALLAKLRSVEALSEEEYRGA
                     LEEPILVQGRGAPEPPPAG"
     gene            2957..3229
                     /locus_tag="VFD06_02320"
     CDS             2957..3229
                     /locus_tag="VFD06_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZN02400.1"
                     /translation="MPDITAVRARIKTLIVESLHLQGIAPADIGDDQPLFGDGLGLDS
                     VDALEMMVALEKEYGIRIDGHQVGKETFTSVSTLAAFVQERLPAAS"
     gene            3226..3960
                     /locus_tag="VFD06_02325"
     CDS             3226..3960
                     /locus_tag="VFD06_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN02401.1"
                     /translation="MSAPRPVALVTGGSRGIGRAVVQTLAAQGWIVAFTWREREEEAR
                     RLAEEIGGAGRPFRFDLRDRARAGDLVREVEEQVGPIEGLVNNAGVRRDGLLATLADD
                     GWDELIDANLGGVFRCCRAAVPRMLHRRRGSIVNVSSMTAVHGLAGQAAYGAAKAGIL
                     GLTRSLAREVGSRGVRVNAVIPGFVPTEMVADIPPERVKALRSAEALPAGVTTGGVAG
                     AVAFLLSDAAASITGQTLVVDAGLLA"
     gene            3971..5143
                     /locus_tag="VFD06_02330"
     CDS             3971..5143
                     /locus_tag="VFD06_02330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN02402.1"
                     /translation="MLPAAGGRDERLARLLPPDHLTIFDERFIRSCELFEEYVRRLAV
                     STARALRLPDAAQAPGTAMEIAARAGLDPERAQAPVGWLLATLASAGAATREADGRFR
                     IAAAPDEPDRREVEEAQAAHDPSCLPSYRLADAAAALYPDVLRGGVTGEAALFSPERF
                     SLWAAYFSNDNPIYAVSNRVGALAAERVLPRGGGEVLEIGGGLGSATAALFQRLEETG
                     GSAAVRSYRFTEVAPSFLRRGQRALTALLPGAPLVYGRLDMDRPFSEAGIEPGAVSLV
                     YGVNTLHVARDLECTLGEIRRALAPGGLLVLSECVRPFPGRPIYVEFLFNLLESFRTP
                     RLVDPWRPHGGFLAPEQWKAGLEAGGFRDVAIVPDIARIREICPACVVASLTAVRA"
     gene            5107..>5509
                     /locus_tag="VFD06_02335"
     CDS             5107..>5509
                     /locus_tag="VFD06_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02403.1"
                     /translation="MRRRLPHRGARLIPLEIPASGTLFEGHFPGRPILPGVGLLDLAL
                     RALPAGPGTAGLRAVDLLRLRSIVVPGERLALEATPPGPDGEVRLEVRRGSEVVANGT
                     LKLGDPGGPSPVGGATAGAAGVPSAAAGVPHL"
BASE COUNT          723 a         1969 c         1978 g          839 t
ORIGIN      
        1 ccgacgtcgg cccgatgccg atcgtcctga gcaccgcctc gatggagcgt tacggcggct
       61 cgagcacccc gaccttcgtg ttcatcgatc gggccgggat cgtccgaggc tacacgccga
      121 cccgcctcac cgaggcggag ctggaccgga ggctccaggc ccttctgcgc tgacctcggc
      181 ccagatcgat cggtgccggg tctcgggaag cagccaggcg ccaatcacga aggtcatcgc
      241 cgcgatggcg atcggtgcgg ggtcgggggc gctcatgggg gcgcatcata gcgacacgcc
      301 gacgaccggg agaaagaaaa agccccggcc gccgaagcga ccggggcttg ctgaagcccg
      361 atgggtcgga cgcgatcggt tacttgccca ggcggaaggt gaagccgccg gccaccgtcg
      421 tgttggtggt ctcttcctcg aacgtgtcct ggttgatcat cgacacgtcg aaacggaacg
      481 cggcgcgctt cgacttgccg aagaagaagc ggctgccggc gccgatctgg tacgccgtgc
      541 cgctgtcgtc cacggagcca aagccttccg cgtcgatgtc cgtgctcgcc tgacccaggc
      601 cgaccagcac gtacggtttg atctccttgc ccggcatgaa gttgaacacg ccgttcacca
      661 tcatgatgtc ggtcgaagcc ttgatggact cgccgatctc ctcgaccgtg gtgtccgagg
      721 agatgtactg cccttcgagc tggaacaggt tggtgaagaa gtacccgccg cggagcgaca
      781 ggctgtcgga cgaatcgaac tcggccgcgt cgttgtcgta cgacgtgctg ccgtagtcga
      841 cgccgatctc tccgttgccc ttctcgatgc cggccagcgc cggcgccgag aagacgagca
      901 cacccagaaa cgcgaggctg acaagcttct tcatggttcc tcccccatat gggtttgtga
      961 tggcaggttc cgtccgccat cgagcaagaa cctagggcgg aggatcgagt cgctgcaagc
     1021 gaatcacaac accgataggg aagaacgaat gcgggatcac ggattcgtcg cgacgcagcc
     1081 gtgacaacct cgaaacctgt ggcattctga gggatgcccc atccttatag atccaggcgc
     1141 ctcctgcgca tcctggccgt ggtggcggtc ctgctcgtgc tcgccgcgct cgccgcacgg
     1201 ccgtcgctcg agcgcaccct ccgatcccgt ctcgaggcgg agggggcgcg ccggggcctc
     1261 gaggtccgcg tcgagagggt gcggctcggt ctctggccgc cgctccggct gcaccggatc
     1321 gcggtcgaaa aggcgggccg gatgcggctc gaagccgagg acctggacgt ctggtggcgc
     1381 ggccggcccc gggtcgagat cgtcaacgcc gtgctgcgcg gccccgcggg gctcgcgatc
     1441 cacgccgagt ggaccgtctg ggacgtcctc gcggcggatc gcgatcacct gcgtgtggcg
     1501 ctggcacggc cggcgaacgg gctggtgctc gaccggacca cggcgccagg ccggacgacg
     1561 tggaccgtgc aggccaccga cctcgcgacc gggcgcctgc tggaggtacg ccggcgcgac
     1621 cgcctgatcc tcgacggcgg gacgctccgc ggctcgctgc agtgtctcga ggaggcgggt
     1681 gaggtccgct tcgacctcga cgtcggcgcg cagtccgcgc gcctgccggc gctcgctccg
     1741 gccgcggctc agcccgatca ggatcccttc gacgagcggg agatggggga gccggtgcag
     1801 atcggcctcc aggcgaccgg cgcctggaac cgggccgacg gggtcctcga catcccgcac
     1861 tacggcgcca cgctcgccgg agcgtcggcc tccggaacgc tgtcgctgcg cgcgatcggc
     1921 cgcgacccgg cgatcgacct gtcgctcgag gtcgagaaga tcgatttcgc gcagctgctg
     1981 cgggcctcgg ggctgccgcc gccggagggc atggctgcgc ccggcgccgc cgcgtccgcc
     2041 gatccctcca cgggggcgcc ggacgtcggt ctcggttcgg cgtcgctgtt cgccacggcg
     2101 caggggcgtc tctccgatcc cgcctcgttc gtcgtgacgc agaatctccg cttcacccct
     2161 ccggcccggt tgccgcgcgc gatcgaacgc ctgcggggcg acttcgtcca cgaggtcggg
     2221 ctccgttccg gcgcgcgccg cggcatcgag gtctcctccg cctcgccgga cttcatcccg
     2281 ctccaggacg tgccgccgct cttccgccgc gcgctcctcc tggcggagga cgccggcttc
     2341 tacagccatc cgggagtcga tctgcgggaa gtcccggcgg cgctcctgac cgatctcgcc
     2401 cgcggggggg cggtgcgggg cgcctcgacg atcacccagc agctcgccaa gaacctgttc
     2461 ctgtcgcgcg acaagaacgt cggccgcaaa cttcaagaat tgtcgctggc gctgctgctc
     2521 gagtcgtccc tggggaagga tcgcatcctg gagatctacc tgaacgtgat cgagtgggga
     2581 ccggacctct tcggcctgcg gccggccgcg cgagcgtatt tcggccgcga gcccgcggag
     2641 ctcaccccgg cgcagacagc gtttctcgtg tcgctcatcc ccgcgccggt gaagtaccag
     2701 gtctccttca aggacggcac gcccggaccg gggctgcggc agctgatgga cgcgctcctc
     2761 gccaagctgc gctcggtgga ggcgctgagc gaggaggagt accggggggc gctcgaggag
     2821 ccgatcctcg tccaggggcg cggagccccg gagccgccgc cggcggggtg aggcgcagcg
     2881 cgagcgtcac cggggtctga ggacaggctc ccaggcgccg ggtggacacg tcggtttggt
     2941 ctatactttt cgctccatgc ccgacatcac cgcggtccgc gcccggatca agaccctgat
     3001 cgtcgaatcg ctccacctgc aggggatcgc ccccgccgac atcggcgacg accagccgct
     3061 gttcggcgac ggactcggcc tcgactcggt ggacgccctc gagatgatgg tggcgctcga
     3121 gaaggaatac ggcattcgca tcgacggtca ccaggtcggc aaggaaacct tcacttccgt
     3181 cagcaccctg gccgccttcg tccaggagcg cctgccggcc gcgtcgtgag cgcgccccgg
     3241 ccggtcgccc tcgtcacggg cgggagccgg gggatcggtc gcgccgtcgt ccagacgctc
     3301 gcggcgcagg gctggatcgt cgccttcacc tggcgcgaac gggaggagga ggcgcgccgt
     3361 ctcgcggagg agatcggcgg agcgggccga cccttccgct tcgatctccg ggaccgggcg
     3421 cgcgccggcg atctcgtccg cgaggtcgag gagcaggtcg ggccgatcga ggggctcgtg
     3481 aacaacgccg gggtgcggcg cgacggcctc ctcgcgaccc tcgcggacga cggctgggac
     3541 gagctaatcg acgccaacct cggcggagtg ttccgctgct gccgcgcggc cgtcccgcgc
     3601 atgctccacc ggcgccgcgg ctccatcgtc aacgtctcgt ccatgacggc ggtgcacggc
     3661 ctcgccgggc aggccgccta cggcgccgcg aaggcgggaa tcctcggcct gacgcgcagc
     3721 ctggcgcgcg aggtcggttc gcgcggcgtg cgggtgaacg ccgtgatccc cgggttcgtg
     3781 ccgaccgaga tggtcgcgga catccctccc gagcgggtga aggcgctccg ctccgccgag
     3841 gcgctgccgg ccggcgtgac gacgggcggg gttgcggggg cggtcgcctt cctgctctcg
     3901 gacgccgccg cctcgatcac cggacagacc ctcgtggtcg acgccggcct gctcgcgtag
     3961 ggaaccggga atgctgcccg cggccggcgg acgggacgaa cgcctggcgc ggctgcttcc
     4021 gccggatcac ctgacgatct tcgatgagcg cttcatccgc tcgtgcgagc tgttcgagga
     4081 gtacgtccgg cgtctggcgg tctcgaccgc ccgcgcgctt cggcttccgg acgcggcgca
     4141 ggcgccggga acagccatgg agatcgccgc gcgcgccggg ctcgatccgg agcgggccca
     4201 ggctccggtc ggctggcttc tcgcgaccct cgcctcggcc ggagccgcga cgcgcgaggc
     4261 ggacggacgc ttccgcatcg cggcggcgcc ggacgagccg gatcggcggg aggtggagga
     4321 ggcgcaggcg gcgcacgatc cctcctgcct gccgtcgtac cggctggccg acgcggcggc
     4381 ggcgctctac ccggacgtcc tgcgcggggg tgtcaccggg gaggcggcgc tcttctcccc
     4441 ggagcggttc tccctgtggg ccgcctactt ctccaacgac aacccgatct acgccgtcag
     4501 caaccgcgtg ggcgctctcg ccgcggagcg cgtcctgccg cgcggcggcg gcgaggtcct
     4561 cgagatcggc ggcgggctgg ggagcgcgac ggcggccctt ttccagaggc tcgaggagac
     4621 cggcggaagc gcggcggtcc gctcctaccg cttcaccgag gtcgccccgt cgttcctgcg
     4681 ccgcggacag cgggccctca ccgcgctcct tccgggcgcg ccgctcgtct acggccggct
     4741 cgacatggat cgcccgttct cggaggcggg gatcgagccc ggggcggtgt cgctggtcta
     4801 cggcgtgaac accctgcacg tcgcccgcga tctcgaatgc accctcggag agatccgccg
     4861 cgccctggct ccgggcggcc tcctcgtcct ttcggaatgc gtgcgcccct tcccgggccg
     4921 gccgatctac gtggagtttc tgttcaacct gctcgagtcg ttccgcacac cccggctcgt
     4981 cgacccctgg cggccgcacg gcggcttcct cgccccggag cagtggaagg ccgggctcga
     5041 agccggcgga ttccgcgacg tggcgatcgt ccccgacatc gcgcgcatcc gggagatctg
     5101 tccggcgtgc gtcgtcgcct ccctcaccgc ggtgcgcgcc tgatcccgct ggagatccct
     5161 gcctcgggaa ccttgttcga agggcatttc cccgggcggc cgatcctgcc cggtgtcggg
     5221 ctgctcgatc tggcgctgcg ggcgctgccg gccgggcccg gcaccgccgg cctgcgggcc
     5281 gtcgatctcc tgaggttgag gagcatcgtc gtgccgggag aacggctggc gctcgaagcg
     5341 acgcctcccg gtccggacgg cgaggtgcgc ctggaggtgc gccgcggctc ggaggtcgtc
     5401 gcgaacggta ctttgaagct cggcgatccg ggcggcccct cgccggtcgg gggcgccacg
     5461 gcgggagccg ccggggtgcc ttccgccgcc gccggggttc cgcatctcg
//
LOCUS       DASRIQ010000095         6530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_36442, whole genome shotgun sequence.
ACCESSION   DASRIQ010000095 DASRIQ010000000
VERSION     DASRIQ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36442"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VFD06_02340"
     CDS             <1..955
                     /locus_tag="VFD06_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02404.1"
                     /translation="AKQAEVILAVVAIREGHLNEAEAGLRRALAIDPNLQTAHTKLGE
                     VLQKQGRDDEALAEFERAVAIAPLSPIEHNHIGNLHRKHGRLPQAMQAYRDSIRADPR
                     GAGGAYNNLGLCLQAQGKLDEAEALYAKGLAIRPENPVLRNSLATLRMLQGRPEEALV
                     EVERAVAADPDWPVAQGNKATLLFELGRMQDARPAFERWVALEPDSIDGRLGWALTLL
                     ATGATDDAATQFQEVLKRDPKNIRAHVALGEMYLRRGDLARAQEHLEAAVKTGEPVPR
                     AWNSLGDVYEKRGLRDRAAEAYRRSLAIDPKQPEIRARLNR"
     gene            complement(1108..2109)
                     /locus_tag="VFD06_02345"
     CDS             complement(1108..2109)
                     /locus_tag="VFD06_02345"
                     /inference="COORDINATES: protein motif:HMM:NF017003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HZN02405.1"
                     /translation="MQSPKRNDPRRAGTMAAVAALALIASGSPALAAGTDYQGWFVAL
                     DAAMTQPTGLDQSAAVLVDPITFQTTRVVIDNDADLSFAIKAGYSWGELGGLEVSYWS
                     FDNDDETSTTDAVNYVYPSVFGYGNYVGSYGLYAPPVTYSTSSGVKANVIDVDYFRAM
                     DIGERFTVKWLAGLRTASFEEDLGFNGSDTSGAYYIESKHIESDAFGIKVGAVLSFGF
                     TDRFALQGKMAFSFLQGDTEAVKTITSGIAGSDRVSADADNVRGEIRDYDLRAVWMWE
                     NLDVYLGYGGQTWDGLVSDPLGSGSCCSVSTYNTSTRDSIGFDSLHAGIVFRFGGGG"
     gene            complement(2270..2671)
                     /locus_tag="VFD06_02350"
     CDS             complement(2270..2671)
                     /locus_tag="VFD06_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02406.1"
                     /translation="MQTLFESILVLVLLLLVFRVSLTSRRHAIRLRGGLVALNILCAM
                     LGATLVACLLRDALRRSGVVPAEPLAAALVRMLPFTFAALGGLAGFVLLGNLWRRLLQ
                     NGPASARTWLRRAEFAVAIAAGLGLLARLLR"
     gene            2697..3722
                     /locus_tag="VFD06_02355"
     CDS             2697..3722
                     /locus_tag="VFD06_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02407.1"
                     /translation="MRCVARIVLAASFAALACGCGGGRDPGSGEAGNAPFLVVSNRGG
                     AWRVYETGRDGRGARLVGSPRADEASYTDTQPARLPDGAIAFVSDRGGRPALWLRPRG
                     AEEARPVLGMPAAAGGAAPNAGTSDSDPAPFGADGIVFARAEDPGGGADPAQAPATVG
                     RDLYLARRDGTGLRRLTTDPADDGAPCVLPDGRSIVFASARRGGPELFRLDASAADPE
                     ATAAPLRSLDRAVSDDAPACLPDGSILFARSTGGGPSQVFQARFTDRGLAARQITDAA
                     TLPYGAGEPVPLADGTILLTAGPGPANGGPRYAVYGISGGGYNLARVTRERAGYEDLT
                     RRLRPPR"
     gene            complement(3751..3972)
                     /locus_tag="VFD06_02360"
     CDS             complement(3751..3972)
                     /locus_tag="VFD06_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZN02408.1"
                     /translation="MARGTIAKLVRDRGFGFIRGNDGKDVFFHRSGLANLNFDDLEQG
                     TTVDFEVENSPKGPRANRVRLANVQNAVE"
     gene            complement(4089..6206)
                     /locus_tag="VFD06_02365"
     CDS             complement(4089..6206)
                     /locus_tag="VFD06_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZN02409.1"
                     /translation="MTTEKKANRLARETSPYLRQHAHNPVDWFPWGPEALEKAKAEDK
                     PIFLSIGYSACHWCHVMERESFENPAIAALMNERFVNIKVDREERPDLDEIYMAATQM
                     LSRQGGWPNSVFLTSKLEPFFAGTYFPPEARYGRPGFPDILRGVAEAYATKREEIGQV
                     ASEVAGQIRALSEMTASKEAVGPKILSRAFGEIAGRFDPVHGGFGGAPKFPHSMDVSF
                     LLRYHRRTGNAEALRMAVVSLDRMARGGLVDQVGGGFHRYSVDAQWLVPHFEKMLYDN
                     ALLARAYVEAAQAIAPLGVSHALPEGGGPPAAFFRQVARDTLDWVLREMLLPEGGFAS
                     SLDADSEGEEGRFYVWTLEDIEQAVGRSEATFVAGTYGATREGNFEGRRNVLSLPLGI
                     AEMARAQRTDEAEVRLRLAGARARMLQARERRPRPGRDDKVLADWNGLMIGALAFAGR
                     VLDDRRYVDAAARAAEMVLGRMTKDGRLLHSFLGGEARHAAYLTDYAAMLQACLDLYE
                     ATFDPRWVEEAERLARQAIDLFRDPKDGGFYFTARDHETLLARTREGNDGALPAGVSL
                     LALALPRLAALDGDAALAPLVDGILRLYRERVERFPSAFGAMLCAVDQFLDPPTQIVL
                     AGSDDAPGLPGLKAVVRERFLPNTVLAGSGGDGESPIALLRGKSKVEGRAAAYVCEGG
                     ACRAPVTAADDLARLLVIAPAAK"
     gene            6294..>6530
                     /locus_tag="VFD06_02370"
     CDS             6294..>6530
                     /locus_tag="VFD06_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02410.1"
                     /translation="MRPNLFAVIVLCACALAADRPVVLTDNAGALSKEEAAALAAEVA
                     PIVEEIRGAKFKHPVPVEIVDDQAARAHFKTRLQE"
BASE COUNT          978 a         2291 c         2379 g          882 t
ORIGIN      
        1 cgccaagcag gcggaggtga tcctcgccgt ggtggcgatc cgggaggggc acctgaacga
       61 agcggaggcg ggattgcggc gcgcgctcgc gatcgatccg aacctccaga cggcgcacac
      121 caagctcggt gaggtgctgc agaagcaggg gcgcgacgac gaggcgctgg ccgagttcga
      181 gcgggcggtc gccatcgccc ccctgagccc gatcgagcac aaccacatcg gcaacctgca
      241 ccgcaagcac ggccgcctgc cgcaggcgat gcaggcctac cgggattcga tccgcgccga
      301 cccgcgcggc gccggcggcg cctacaacaa cctcgggctc tgcctgcagg cccaggggaa
      361 gctcgacgag gcggaggcgc tctacgccaa gggcctggcg atccggccgg agaacccggt
      421 gctgcgcaac tcgctcgcga cgctgcggat gctgcagggg cggccggagg aggcgctggt
      481 ggaggtggag cgggcggtcg ccgccgatcc cgactggccg gtggcgcagg ggaacaaggc
      541 gacgctcctg ttcgagctgg ggcgcatgca ggacgcacgc cccgccttcg agcggtgggt
      601 ggcgctggag ccggactcga tcgacgggcg gctcggctgg gccctgacgc tgctcgcgac
      661 cggcgccacc gacgacgccg cgacgcagtt ccaggaggtg ctgaagcgcg atccgaagaa
      721 catccgcgcc cacgtcgctc tcggagagat gtacctgcgc cgcggcgatc tggcgcgggc
      781 ccaggagcac ctcgaagcgg cggtgaaaac cggcgagccg gtgccgcggg cctggaattc
      841 cctcggcgac gtctacgaaa agcgcggtct cagggatcgc gccgcggagg cctacaggcg
      901 atcgctcgcc atcgacccga agcagcccga gattcgcgcg cgcctcaatc gctgacggaa
      961 cggccccaat cgcaccttgc gagacacgcc gacttcgggg cggccgtcag ggatgggcgg
     1021 gtcggggaaa cgggaacgcc cccgccgggg cgattcccgt ttcccctgag gcgctgagaa
     1081 cgaagcgctg tcaggctctg gcgagactca tcccccgccg ccgaagcgga acacgatgcc
     1141 ggcgtgcagc gagtcgaacc cgatcgagtc gcgcgtgctg gtgttgtagg tggacacgga
     1201 acagcagctg ccgctcccca gcggatcgga gaccagtccg tcccacgtct ggcctccata
     1261 gcccaggtag acgtcgaggt tctcccacat ccacacggcc ctcagatcgt aatcgcggat
     1321 ctcgccgcgc acgttgtccg cgtcggcgct gacccgatcg gatcccgcga tgccggaggt
     1381 gatcgttttc accgcttcgg tgtccccctg caggaaggag aaggccatct tcccctgcag
     1441 ggcgaagcga tcggtgaagc cgaacgacag caccgcgccg accttgatcc cgaaggcgtc
     1501 cgactcgatg tgcttcgact cgatgtagta cgcgccggac gtgtcgctgc cgttgaagcc
     1561 caggtcctcc tcgaacgacg cggtccggag gccggcgagc cacttcaccg tgaaccgctc
     1621 cccgatgtcc atggcacgga agtagtcgac gtcgatgacg ttcgccttca cccccgagga
     1681 ggtggagtag gtgacggggg gcgcgtacag gccgtacgac ccgacgtagt tgccgtatcc
     1741 gaagaccgac gggtacacgt agttcacggc gtcggtggtc gaggtctcgt cgtcgttgtc
     1801 gaagctccag tacgagacct ccagcccgcc cagctcaccc cacgagtagc cggccttgat
     1861 cgcgaacgag aggtcggcat cgttgtcgat gacgacgcgg gtggtctgga aggtgatcgg
     1921 atccacgagg acggcggcag actgatcgag cccggtcggc tgcgtcatcg ccgcgtcgag
     1981 cgcgacgaac cagccctggt aatcggtgcc cgccgccagc gcggggctgc ccgaggcgat
     2041 cagcgcgagg gcggcgaccg ccgccatcgt tcctgcgcgc cgcggatcgt tccgtttcgg
     2101 cgactgcatg cgagagtcct ccgtcggtaa ggggaaaggg gccggcgccc gtaccgccgg
     2161 cgggattcgc gtcgcccggg aggccgcgta ctcctccttt ccgggccgag gtcccggcga
     2221 ggcggacggt gctatatccc agcgcggtcg ccggcgtcaa cagcggcgct cagcgcagca
     2281 ggcgcgcgag caggccgagc cccgccgcga tcgcgacggc gaattcggcg cggcgcagcc
     2341 aggtgcgcgc cgacgccgga ccgttctgga ggaggcgccg ccagagattc ccgagcagga
     2401 cgaaccccgc gagcccgccc agcgccgcga aggtgaacgg cagcatgcgg acgagcgccg
     2461 ccgcgagcgg ctccgccggc acgacgcccg agcgacgcag cgcgtcgcgc agcagacagg
     2521 cgacgagcgt cgcgcccagc atcgcgcaca ggatgttgag ggccaccagc ccgccgcgca
     2581 gccggatggc gtgccggcgg ctcgtcagcg acacccggaa cacgagcagg agcagcacca
     2641 ggacgaggat cgactcaaac agcgtctgca tcgcggttcc ggataggatg cggcgcatgc
     2701 ggtgcgttgc gcggatcgtc ctcgccgcct cgttcgcggc tctggcctgc gggtgcggcg
     2761 gcggccggga cccggggagc ggtgaggccg ggaacgcacc attcctcgtc gtctcgaatc
     2821 gcggcggcgc gtggcgcgtc tacgagaccg ggcgcgacgg ccgcggcgcg cgcctcgtgg
     2881 gatcgccgcg cgccgacgaa gcgtcctaca ccgacacgca gccggcgcgg ctcccggacg
     2941 gggcgatcgc gttcgtcagc gatcgcggtg gccgcccggc cctctggctg cgtccgcgcg
     3001 gggcggagga ggcgcggccg gtgctcggga tgccggcggc cgcgggcggc gccgcgccca
     3061 acgcggggac gtccgattcc gacccggctc cgttcggcgc cgacgggatc gtcttcgccc
     3121 gcgcggagga ccccgggggt ggcgccgatc cggcgcaggc gcccgcgacg gtcggccgcg
     3181 acctgtacct cgcgcgacgc gacgggaccg gcctgcgccg gctgacgacc gatcctgccg
     3241 acgacggggc gccgtgcgtc ctgccggacg ggcgctcgat cgtcttcgcg tcggcgcggc
     3301 gcggaggccc ggagctcttc cgcctcgacg cgtccgccgc cgatccggag gcgaccgcgg
     3361 ccccgctccg ctcgctggat cgcgccgtgt ccgacgacgc tcccgcctgc ctgccggacg
     3421 gatcgatcct gttcgcgcgc tccacgggag gcggaccgtc ccaggtgttc caggcgcgct
     3481 tcaccgatcg cgggctggcg gcgcggcaga tcaccgacgc ggcgacgctg ccgtacggcg
     3541 cgggggagcc ggtcccgctg gccgacggca cgatcctgct gacggcgggg ccggggccgg
     3601 cgaacggcgg accgcgctac gcggtttatg gcatctccgg aggggggtac aacctcgctc
     3661 gcgtgacgcg cgagcgcgcg ggctacgagg acctgacgcg ccgcctccgc ccgccgcgct
     3721 gagcgggtgg gacgagcggg ggaacgcgat ttactcgacg gcgttctgaa cgttcgcgag
     3781 gcggacgcga ttcgcccgcg gtcccttcgg gctgttctcc acctcgaaat cgacggtcgt
     3841 tccctgctcg aggtcgtcga agttcaggtt cgccagaccg gagcggtgaa agaaaacgtc
     3901 cttgccgtcg ttgccgcgga tgaacccgaa cccgcggtcg cggaccagct tcgcgattgt
     3961 gccacgtgcc atggatgaga cctcagactg caaccctgcc gtcagggttc gctggcgagg
     4021 attgtcggcg tccgggacag gggctgtcaa gacggtcgca aatcgatccg cgcgaatatg
     4081 tcatcgcact atttcgctgc cggggcgatc acgagcaggc gggcgagatc gtccgcggcg
     4141 gtgaccggcg cgcggcacgc gcccccctcg cagacgtacg cggcggcgcg tccctcgacc
     4201 ttcgatttgc cgcggaggag cgcgatcgga gactccccgt ccccgcccga tcccgccagg
     4261 accgtgttgg gcaggaagcg ctcgcgcacc acggccttca gtcccgggag tccgggggcg
     4321 tcgtccgatc ccgcgaggac gatctgcgtc ggcggatcga ggaactgatc gaccgcgcac
     4381 agcatcgcgc cgaaggcgga cggaaaacgc tcgacgcgct cgcggtacag gcggaggatt
     4441 ccgtcgacga gcggcgcgag tgcggcgtcg ccgtcgagcg ccgcgaggcg cggcagcgcg
     4501 agcgccagga gcgacacgcc ggcgggaagc gccccgtcgt tcccctcgcg cgtgcgggcc
     4561 agcagggtct cgtggtcgcg cgcggtgaag tagaagccgc cgtccttcgg atcgcggaag
     4621 agatcgatcg cctgccgggc cagccgttcc gcctcctcga cccagcgcgg atcgaaggtg
     4681 gcctcgtaca ggtcgaggca cgcctgcagc atcgcggcgt agtcggtcag gtacgcggcg
     4741 tgccgcgcct cgccgccgag gaacgaatgc aggagacgac cgtccttcgt catgcgcccg
     4801 aggaccatct ccgcggcgcg cgcggccgcg tccacgtagc gccggtcgtc gaggacgcgg
     4861 ccggcgaacg ccagcgcgcc gatcatcagg ccgttccagt cggcgagcac cttgtcgtcg
     4921 cggccggggc ggggacgccg ctcgcgcgcc tgcagcatcc gcgcccgggc tcccgcgagg
     4981 cgcagccgca cttccgcctc gtcggtccgc tgcgcccgcg ccatctcggc gatcccgagc
     5041 gggaggctca ggacgttgcg ccgcccttcg aagttcccct cccgcgtggc gccgtaggtg
     5101 ccggcgacga acgtcgcctc ggagcggccg accgcctgct cgatgtcctc gagcgtccag
     5161 acgtagaagc gcccctcctc cccctcgctg tcggcgtcga ggctcgaggc gaagccccct
     5221 tcgggcagga gcatctcgcg caggacccag tcgagcgtgt cgcgcgccac ctggcggaag
     5281 aaggcggccg gcggtccgcc cccctcgggc agcgcgtgcg agacgccgag cggggcgatc
     5341 gcctgggccg cctcgacgta ggcgcgggcg agcagcgcgt tgtcgtacag catcttctcg
     5401 aagtgcggga cgagccactg cgcatcgacc gagtagcggt ggaagccgcc gccgacctga
     5461 tcgacgaggc cgccacgcgc catccggtcg aggctcacca ccgccatccg cagcgcctcg
     5521 gcgttgccgg tgcgccggtg gtagcggagc aggaaggaca cgtccatgct gtgcgggaac
     5581 ttgggggcgc cgccgaagcc gccgtggacc ggatcgaagc ggccggcgat ctcgccgaag
     5641 gcgcgcgaga ggatcttcgg cccgaccgcc tccttcgagg cggtcatctc cgacagcgcg
     5701 cggatctgcc ccgccacctc ggaggcgacc tgcccgatct cctcgcgctt cgtcgcgtac
     5761 gcctcggcca cgccgcgcag gatgtccggg aacccgggcc ggccgtagcg cgcctccggc
     5821 gggaagtacg taccggcgaa gaacggctcg agcttcgacg tcaggaagac cgagttcggc
     5881 cagcccccct ggcgcgacag catctgcgtc gccgccatgt agatctcgtc gagatcgggg
     5941 cgctcctcgc ggtcgacctt gatgttgacg aagcgctcgt tcatcagcgc cgcgatcgca
     6001 gggttctcga acgactcgcg ctccatgacg tggcaccagt ggcaggcgga gtagccgatc
     6061 gacaggaaga tcggcttgtc ctccgccttc gctttctcaa gcgcctccgg gccccatgga
     6121 aaccagtcga cgggattgtg ggcgtgctgg cggagatacg ggctcgtctc ccgcgcgagg
     6181 cggttcgcct tcttctcggt ggtcatcgga agaagaaagg ctatcacgtc gcgctgacca
     6241 tcacgtgact cgtccaaacc ggcctggcgc gggggacggg ctatcatggc ggcgtgagac
     6301 ccaatctctt cgccgtcatt gtcttgtgcg cgtgcgctct ggcggccgac cggcccgtcg
     6361 tgctcacgga caacgcgggg gcgctgtcga aggaggaggc ggcggcgctg gcggcggagg
     6421 tggcgccgat cgtcgaggag atccgggggg cgaagttcaa gcacccggtg ccggtggaga
     6481 tcgtcgacga ccaggcggcg cgggcgcact tcaagacccg gcttcaggaa
//
LOCUS       DASRIQ010000096         4233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1853151, whole genome shotgun sequence.
ACCESSION   DASRIQ010000096 DASRIQ010000000
VERSION     DASRIQ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853151"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VFD06_02375"
     CDS             complement(<1..1322)
                     /locus_tag="VFD06_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZN02411.1"
                     /translation="MREVCRSRPAYGKRLLFASTLFTGLFVADLFILARLAFKDLGQQ
                     VIDHAFRESFDALEPRPGPVAPMLASDAPPGAGSAAECIVGAPVSRDGGIPCRPAAGG
                     PARPTGFFRALTVRLERALTDTRGRVLWRGVWEGSLPRDGGQTERHFEPGHERVRETW
                     DVDGQLRPVLALRQAIGSHPTEIREIGIPEELIERELQTLRSDLQRRIWIGALGAVIV
                     LVIAFLYVLRLLRRTRLLEAQAQMDDRLAYVGGLAAGLAHEIRNPLNVLSMNLQMLEE
                     DLVHRLGGEGAGDTRQYLGTLQGEIRRLSSLVDNFLSYARPSAPRFESRNLNEVVSGT
                     LQLVRPQFESQGVALREQPSPVLPMVDLDEGLIRQALMNILMNAVQILKAGGTVTVET
                     GIAPDGGVQVAITDDGPGIGPADRERIFEVFYSSRPGGTGLGLPIAAR"
     gene            1586..3487
                     /locus_tag="VFD06_02380"
     CDS             1586..3487
                     /locus_tag="VFD06_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN02412.1"
                     /translation="MDREATRSGSRIAFRAAVLASALVLLIVPASAPRAEERKHSWEV
                     GAFAGYTIFGHELQVDDSADYGLRVGWYLSPPYEIELQYYKSFSSTLEDGDSTLLTDS
                     SVFAENPSREWTATAWTARFTINPRNDRRRFKPYAQLGIGQLSWEPSPALTSGQEGDL
                     DAWVFSVGAGARYRFGAYTQFRVEFEDLYAVSEVYSNFHLTAGLSWVFGGGKPADSDA
                     DGVLDLKDRCPDTPAGALVDKHDGCPWDIDRDGVMEGVDQCAATPAGWPVDEKGCPLD
                     SDGDAVPDGLDKCPDTMKAAVVDSTGCPADADKDGIFDGLDRCPGTPQGAIVDPIDSP
                     TPGCPHDADNDSVFDGVDQCPLTIVGATVDEKGCPKDSDGDKVLDGLDQCPDTIKGSK
                     IDKEGCPRIRLDKTESQIVPNVKFHGMELYPGSDAWLQLLIEAANYWPDVVFEVGVYT
                     DNEGGVAANRAAAQRRAEVLRAWLVNQGIPDRRFVVKAYGPVNFIADNSTEEGRDKNR
                     RIEAKRLSGDIRKHPKPEPEAPPEPAPAPTPEQTPPAEPPPAGQEKPPAAAPEQTPPA
                     PAPEPPPPPPPADPEPKPPADPEPKPPAEPDPKPPAEAAPGGDGQAPAGDPPPADAPS
                     GGRAAPSLH"
     gene            complement(3503..>4233)
                     /locus_tag="VFD06_02385"
     CDS             complement(3503..>4233)
                     /locus_tag="VFD06_02385"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZN02413.1"
                     /translation="LVCWHRLTIRPGSMIVTVVGALAASEAADLVHQAIAEWPQDGFG
                     GSEPGALLPWGAAEVVETRRRAQTAQIIGFPTPGVQSPERHALDIVQSVTSGLGGRFF
                     EAVRGKRGLAYAVQSFNFHRLRGGAFLTYLGTSPRDEAEARRVLFLEIATLRHDGPRR
                     DEIERARRHLIGGHAIGMQGNGSRALRYADAEVRGLGAEAVVDYPRHVSTVVDQDVGD
                     AIWRYLDPDRCAIGILRGEAPASS"
BASE COUNT          650 a         1601 c         1456 g          526 t
ORIGIN      
        1 atgcgcgcgg cgatcggcag tcccaggccg gtcccgcccg gccggctgga gtagaagacc
       61 tcgaagatcc gctcccggtc ggcggggccg atccccggcc cgtcgtcggt gatggccacc
      121 tggacgccgc cgtccggggc gatccccgtc tccaccgtca ccgtcccgcc cgccttgagg
      181 atttgcaccg cgttcatcag gatgttcatc agcgcctggc gaatgagccc ctcgtcgaga
      241 tcgaccatcg gcaggaccgg cgagggctgc tcgcggaggg cgactccctg cgactcgaac
      301 tgcggccgca ccagctgcag cgtgccggac accacctcgt tgaggttgcg gctctcgaag
      361 cgcggcgcgg agggacgcgc gtacgacagg aagttgtcca cgaggctcga gagccgccgg
      421 atctctccct gcagcgtgcc gaggtactgc cgcgtgtccc cggccccctc gccgccgagg
      481 cggtgcacca ggtcctcctc cagcatctgg aggttcatgc tcaggacgtt gagcgggttg
      541 cggatctcgt gcgccaggcc ggccgcgagc ccgccgacgt aggcgagccg atcgtccatc
      601 tgcgcctgcg cctcgagcag acgcgtcctc ctcaagagac gcaggacgta gaggaacgcg
      661 atcacgagga cgatcacggc gccgagcgcg ccgatccaga tgcggcgctg cagatcggag
      721 cgcagcgtct gcagctcccg ctcgatcagc tcctcgggga tgccgatctc gcggatctcg
      781 gtcgggtggc tgccgatcgc ctgccgcagc gccagcaccg gccgcagctg cccgtccacg
      841 tcccacgtct cgcggacccg ctcgtgtccc ggctcgaagt ggcgctcggt ctgcccgccg
      901 tcgcggggca ggctcccctc ccagacgccg cgccacagca cccggccgcg cgtgtcggtc
      961 agggcccgct cgaggcgcac ggtcagcgcg cggaagaacc cggtcggccg cgcgggaccg
     1021 ccggcggcgg gccggcaggg aatgccgccg tcgcgcgaga ccggtgcgcc gacgatgcac
     1081 tcggcggcgg acccggcgcc ggggggggcg tcgctcgcca gcatcggtgc caccggcccc
     1141 ggccgcggct cgagcgcgtc gaacgactcg cggaacgcgt ggtcgatcac ctgctgcccg
     1201 agatccttga aggcgagccg ggcgaggatg aacagatccg cgacgaacag cccggtgaac
     1261 agggtggacg cgaacagcag ccgtttgccg taggccggcc gcgatcggca gacttcccgc
     1321 aaagagcgcc ctccgcgcgc cgtatgagtg agtcgaaaca tcaagatagc acaggcggcg
     1381 gcgcctcacc ggccgcggtg ccccctccgc ggatcggcgc gtccgccggg cgccgcatcg
     1441 ttgacagccc ggaaaacgac gtgctagcat ccgccgcttt ttcgatgcca gagacgagtt
     1501 tgccccgccc cgccgacccg ggccgccgtt gatccccgcc ggtcgacggt gatcggacag
     1561 ccgcgacttc gaggaaccag cgcgcatgga tcgtgaggca acgcgatcgg gcagtcggat
     1621 cgcattccgc gccgccgtcc tggcgtcggc gctcgtcctc ctgatcgttc ccgcgagcgc
     1681 cccgcgcgcc gaggagcgca agcactcgtg ggaggtcggc gccttcgccg gctacacgat
     1741 cttcgggcac gagctgcagg tggacgactc cgccgactac ggcctgcgcg tcggctggta
     1801 cctctccccg ccctacgaga tcgagctgca gtactacaag tcgttctcgt cgacgctcga
     1861 ggacggggac agcaccctcc tcaccgactc gtcggtcttc gccgaaaacc ctagccgcga
     1921 atggaccgcc acggcgtgga ccgcgcggtt cacgatcaac ccgcgcaacg atcgccgccg
     1981 cttcaagccg tacgcgcagt tgggcatcgg gcagctctcg tgggagccga gcccggccct
     2041 cacctcgggc caggaggggg acctcgacgc ctgggtcttc agcgtcggcg ccggtgcgcg
     2101 ctaccgcttc ggcgcctaca cgcagttccg ggtggagttc gaggatctgt acgccgtcag
     2161 cgaggtctac tccaacttcc acttgaccgc cggtctgtcc tgggtgttcg gcggaggcaa
     2221 gccggccgac agcgacgccg acggcgtcct cgacctgaag gaccgctgcc ccgacacgcc
     2281 cgccggggcg ctcgtcgaca agcacgacgg gtgcccgtgg gacatcgacc gcgacggcgt
     2341 catggagggg gtcgatcagt gcgccgcgac gccggccggc tggccggtgg acgagaaggg
     2401 ctgcccgctc gactccgacg gcgacgcggt cccggacggg ctcgacaagt gccccgacac
     2461 gatgaaggcg gcggtggtgg actccaccgg ctgcccggcc gacgccgaca aggacgggat
     2521 cttcgacggc ctcgaccgct gccccggcac gccgcagggg gcgatcgtcg atccgatcga
     2581 cagcccgacg ccgggctgcc cgcacgacgc cgacaacgac agcgtcttcg acggcgtcga
     2641 tcagtgcccg ctgacgatcg tcggcgccac ggtcgacgag aagggctgcc cgaaggacag
     2701 cgacggcgac aaggtgctcg acggcctcga ccagtgtccc gacacgatca agggctcgaa
     2761 gatcgacaag gaaggctgcc cgcgcatccg gctcgacaag accgagtcgc agatcgtgcc
     2821 gaacgtgaag ttccacggca tggagctcta ccccggaagc gacgcctggc tgcagctcct
     2881 gatcgaggcg gccaattact ggcccgacgt ggtcttcgag gtcggcgtct acaccgacaa
     2941 cgagggaggc gtcgccgcca accgcgccgc cgcccagcgc cgcgccgagg tgctgcgcgc
     3001 ctggctcgtc aaccagggga tcccggatcg gcgtttcgtg gtcaaggcgt acgggccggt
     3061 gaacttcatc gccgacaaca gcaccgagga agggcgcgac aagaaccgcc gcatcgaggc
     3121 gaagcggctc tccggcgaca tccgcaagca cccgaagccg gagcccgagg cgccgcccga
     3181 gccggccccc gcgccgaccc cggagcagac gccgccggcg gagccgccgc cggccggtca
     3241 ggagaagccg cccgcggcgg cccccgagca gacgccgccg gcccccgccc cggagccccc
     3301 gcctccgccg ccaccggccg atccggagcc gaagccaccg gccgatccag agccgaagcc
     3361 accagccgag ccggatccga agcctccggc cgaagccgct ccgggcggcg acggacaggc
     3421 ccctgcgggc gatccccccc ccgcggacgc gccgtcgggc gggcgcgccg ccccgtccct
     3481 gcactgattt tccgaaagcg gattacgacg aggcgggcgc ctcgcccctg aggattccga
     3541 tcgcgcagcg atccggatcg aggtagcgcc agatggcgtc gccgacgtcc tggtccacca
     3601 ccgtggagac gtggcgcgga tagtccacca ccgcctccgc ccccagcccg cgcacctcgg
     3661 cgtcggcata ccgcagcgcc cgcgagccgt tcccctgcat cccgatggcg tgtccgccga
     3721 tgaggtgccg ccgggcccgc tcgatctcgt cgcggcgcgg cccgtcgtgg cgcagcgtcg
     3781 cgatctccag gaacaggacg cggcgcgcct ccgcctcgtc gcgcggcgag gtgcccaggt
     3841 acgtcaggaa cgcgccgccg cgcaggcggt ggaaattgaa cgactgcacc gcataggcca
     3901 ggccgcgctt gccgcggacc gcctcgaaga accgcccccc cagacccgag gtgaccgact
     3961 ggacgatgtc gagggcgtgc cgctccggcg actgcacccc cggcgtcggg aacccgatga
     4021 tctgcgccgt ctgcgcgcgc cggcgggtct ccaccacctc ggcggccccc cacggcagga
     4081 gcgcccccgg ctccgagccg ccgaagccgt cctgcggcca ttcggcgatc gcctggtgga
     4141 cgagatcggc cgcctcggac gccgccagcg ccccgaccac cgtgacgatc atcgagcccg
     4201 gacggatggt cagccggtgc cagcacacca ggt
//
LOCUS       DASRIQ010000097         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2908472, whole genome shotgun sequence.
ACCESSION   DASRIQ010000097 DASRIQ010000000
VERSION     DASRIQ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2908472"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2457
                     /locus_tag="VFD06_02390"
     CDS             <1..>2457
                     /locus_tag="VFD06_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HZN02414.1"
                     /translation="NTKGVVIDQSPQNLVGGTAPGSRNVIAGGREQASGHGVEITGWP
                     ALNALHNMVQGNYIGTDITGTQRLPNLGSGVDISYADASQIGGTAPGARNLISANLQY
                     GVRTQTSSFVRVQGNILGPDITGTISLSRNGNDGSVGVALDNGTSNSVVGGSVAAARN
                     LASGGVVGILAYGHTSFGNAILGNYVGTDPTGTQAVPNAGHGILVASPNTQVGGPGLG
                     EGNLSSGNNAQSGACGVAIWSEGESCLVQGNILGLDVTGTTPLGNDRGLLINGGAYCR
                     YGGTNPGEANVIAGNDAHGVWIFDPQGNSLPEQDASLDTPKSVPDLATTTSVISVAPS
                     ATNLLVSVKVNVALPHSFDGDLILTLIAPNGTRILLANRRGGAGHDYLTTQFDDLAAT
                     PIGSGAAPFNGAFQPEEPLSVLSGVPIGGTWTLEIADVAGGDAGFLLGWSLDLVYLRT
                     DRSSFQSQLVGNFIGTNAAGASGLGNGGDGIFGERSNILTIGVPGGGNVISGNAGHGI
                     ALNATFCAGNVVQANLIGLKPDGVTPLGNGSDGIAVSGPVGLIGGPGAAFGNRIAWNA
                     GAGVNVVASTLPRQTTIRVNSIHDNGGLGIDLAPLGVTANDSGDGDGGANQLQNHPVI
                     TGAVPSFMNSLLISGTLQSRPNSKYTVDVYGSPQADPSGSGEGRVWLGFANITTDLSG
                     NASWTVLTQNGSVGLLAATATDFAGNTSEFSGLMKNPQEASPAKDMTVAIGAGGTLQI
                     AWTPACGASDHVLYWGTVGSGMIGPGGLNWTSAACGLGTSGTAGVLLGNPPVGSAFYF
                     VMVGQTGAVEGSYGQSSSGG"
BASE COUNT          398 a          894 c          823 g          342 t
ORIGIN      
        1 caacaccaag ggggtggtca tcgaccagtc gccgcagaac ctggtcggcg gcacggcgcc
       61 cgggtcccgc aatgtcatcg ccggggggcg cgagcaggcc tcggggcacg gcgtcgagat
      121 caccggctgg ccggcgctca acgctctgca caacatggtg caggggaact acatcggcac
      181 cgacatcacc ggaacgcagc gtctcccgaa cctgggaagc ggcgtcgaca tctcctacgc
      241 cgatgccagc cagatcggcg ggaccgcgcc gggagcgcgc aacctgatct ccgccaacct
      301 gcagtacggc gtccgcacgc agacgtcgtc gttcgtgcgg gtccagggga acatcctcgg
      361 tcccgacatc accggaacca tctccctcag ccgcaacgga aatgacggca gcgtcggggt
      421 cgccctcgat aatggcacca gcaacagcgt ggtcggaggc agtgtcgccg cggcgcgcaa
      481 tctcgcctcc gggggcgtcg tcgggatcct cgcctacgga cacacctcgt tcggcaacgc
      541 catcctcggg aactacgtcg gcaccgatcc cacggggacg caggcggtgc cgaacgccgg
      601 gcacggcatt ttggtcgctt ccccgaacac gcaggtgggc ggccccggtc tgggcgaagg
      661 gaacctgtcc tccgggaaca atgcccagtc cggggcctgt ggcgtggcca tctggtccga
      721 gggggagtcg tgcctcgtcc agggaaacat cctcggcctc gacgtcaccg ggacgacgcc
      781 gctcgggaac gatcgcggcc tcctgatcaa cggcggggcc tactgccgct acggcggcac
      841 caaccccggc gaggcgaacg tcatcgccgg caacgacgcg cacggagtct ggatcttcga
      901 cccgcagggg aacagcctgc ccgagcagga cgcctcgctc gacacgccga agtccgtgcc
      961 ggacctggcg accacgactt cggtgatcag cgtcgccccc tccgccacca acctgctcgt
     1021 cagcgtgaag gtcaacgtgg cgctgccgca ctcgttcgac ggggatctga tcctgaccct
     1081 catcgcaccc aacgggaccc gcatcctcct ggcgaaccgg cgcggcggag ccggccacga
     1141 ctacttgacg acgcagttcg acgacctcgc cgccacgccg atcgggtccg gcgcggcgcc
     1201 cttcaatggc gcgttccagc ccgaggagcc gctctccgtg ctgtcggggg tgccgatcgg
     1261 cggaacctgg acgctggaga tcgccgatgt cgcgggcggc gatgcgggct tcctcctggg
     1321 ctggtcgctc gacctcgtct acctgaggac ggatcgatcg agcttccagt cccagctcgt
     1381 cgggaacttc atcggcacca acgccgccgg ggcgagcggc ctcgggaacg gcggcgacgg
     1441 catcttcggc gagcgctcga atatcctcac gatcggcgtg ccgggcggcg gcaacgtcat
     1501 ctccgggaac gccggacacg gcatcgcgct gaacgcgacc ttctgcgccg gcaacgtcgt
     1561 ccaggcgaac ctgatcggcc tgaagccgga tggtgtcacc ccgctcggca acgggagcga
     1621 tggcatcgcc gtgagcggcc cggtcgggct catcggcggc cctggcgccg ccttcggcaa
     1681 ccggatcgcc tggaacgccg gcgccggcgt gaacgtcgtg gcgtcgaccc tcccgcgcca
     1741 gacgaccatc cgggtgaact cgatccacga caacggcggg ctcgggatcg acctcgcgcc
     1801 tctcggggtc accgccaacg actccggtga tggggacggc ggtgccaacc agctgcagaa
     1861 ccacccggtc atcaccggcg ccgtcccgag cttcatgaac agcctgctca tctccggcac
     1921 gctccagagc cggccgaact cgaagtacac cgtcgacgtc tacggctcgc cgcaggccga
     1981 tccctccggt tccggcgagg ggcgcgtctg gctcgggttc gccaacatca cgaccgacct
     2041 ctcgggcaac gcctcctgga cggtcctgac ccagaacggc agcgtcggcc tgctcgcggc
     2101 gaccgcgacc gatttcgccg gcaacacctc ggagttctcc ggcctgatga agaatcccca
     2161 ggaggcgtcg ccggccaagg acatgacggt cgcgatcggg gccggcggca cgctccagat
     2221 cgcctggacg ccggcgtgcg gcgccagcga ccacgtcctc tactggggga cggtggggtc
     2281 gggcatgatc ggccccggcg ggttgaactg gaccagcgcc gcctgcggtc tcggcaccag
     2341 cggcaccgcc ggcgtcctcc tcgggaatcc accggtcggc agcgccttct acttcgtcat
     2401 ggtcgggcag accggcgcgg tcgaaggctc ctacggccag agctcgagcg gcggcga
//
LOCUS       DASRIQ010000098         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1596479, whole genome shotgun sequence.
ACCESSION   DASRIQ010000098 DASRIQ010000000
VERSION     DASRIQ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596479"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1312)
                     /locus_tag="VFD06_02395"
     CDS             complement(152..1312)
                     /locus_tag="VFD06_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN02415.1"
                     /translation="MDFSLTEEQELTRRTVREFAEREIAPRSRRMDETQEFPLDLMRR
                     IGGMGLLGVVFPPEYGGAGLTYPDFALIIEELARVDGAIALSVAAHNSLCSNHIYLAG
                     NEEQKQRWLVPLAGGEKIGAWSLTEPTAGSDASGTRTTAVKDGDHWILNGSKTFATHG
                     SVCDVAVVFAVTDKAKGKHGISAFVLERGLAGWRPGKKEDKMGCRASDTAEIVMENCR
                     VPGSHLLGQEGEGFIDALRILDGGRIGIGSLSVGIAQGAMEQAIAYARGRRQFGRPIA
                     EFQAVQWMLADSATEIDAARLLVQRAAAMRAQGEDTNLASAMAKLFASETAVRVCDRA
                     VQIHGGYGYVKDYPVERAYRDAKLTTIGEGTSEIQRLVIARHLIGRAGEQTP"
     gene            1643..2047
                     /locus_tag="VFD06_02400"
     CDS             1643..2047
                     /locus_tag="VFD06_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZN02416.1"
                     /translation="MLTLQQRLNRFLVDPSNAYGPEGVGSWAPPVDIVEEPERLLFRA
                     EIPGVSKDDIDIKVENGTLILRGEKKQELEVEGETSHRVERFYGAFTRSFALPATIDA
                     DKIQARYKDGVLELVLPKAEIAKPRKIQIVAA"
BASE COUNT          347 a          726 c          669 g          356 t
ORIGIN      
        1 ccggtgatgc cgaggagggc ggccttcccc gtccgcccgt agaggcggcg cagcacctcc
       61 accccctcgg cccgtcccgc ctccaccagg ctgatggcgc gcgccagcgc cagcggctcg
      121 ccgcgcgcga cgcgctccgc gagcccttcg gtcacggcgt ctgctcgccg gcgcggccga
      181 tgagatggcg cgcgatgacg agccgctgga tctcggaggt tccttccccg atggtcgtga
      241 gcttcgcgtc gcggtaggcg cgctccaccg ggtagtcctt cacgtagccg tagccgccgt
      301 ggatctgcac cgcccggtcg cacacccgca ccgccgtctc cgaggcgaac agcttggcca
      361 tcgcggaggc caggttggtg tcctctccct gggcgcgcat cgccgccgcc cgctgcacca
      421 ggagacgcgc cgcgtcgatc tcggtggcgg agtcggccag catccactgc accgcctgga
      481 actcggcgat ggggcggccg aactgcctgc gtccgcgggc gtaggcgatc gcctgctcca
      541 tcgccccctg cgcgatgccg accgacagcg acccgatgcc gatccgcccg ccgtcgagga
      601 tgcgcagcgc gtcgatgaat ccctcccctt cctgtccgag gaggtggctt cccggcacgc
      661 ggcagttctc catgacgatc tcggccgtgt ccgaggcgcg gcagcccatc ttgtcttcct
      721 tcttcccggg gcgccagccg gcgagaccgc gctccagcac gaaggccgag atgccatgct
      781 tcccttttgc cttgtcggtg accgcgaaca ccaccgcgac gtcgcacacc gatccgtgcg
      841 tcgcgaacgt cttgctgccg ttgaggatcc agtggtcgcc gtccttcacg gcggtggtgc
      901 gggtgccgga cgcatcgctg ccggcggtcg gctcggtcag gctccaggcg ccgatcttct
      961 cgccgcccgc cagaggaacg agccagcgct gcttctgttc ctcgttcccg gcgaggtaga
     1021 tgtggttgct gcagagcgaa ttgtgggccg cgaccgacag ggcgatcgcc ccgtcgaccc
     1081 gcgccagctc ctcgatgatc agggcgaaat cgggataggt caggcctgcg ccgccgtact
     1141 cgggggggaa gacgacaccc agaagcccca tgccgccgat ccggcgcatg aggtcgagcg
     1201 ggaactcctg ggtctcgtcc atccgccggg agcgcggagc gatctcgcgc tcggcgaact
     1261 cgcgaacggt gcggcgcgtc agctcctgtt cttccgtgag gctgaaatcc aaccggggct
     1321 ccaggtgaag gtgaggaagc gtgccgcaaa gcggaattct atggcagcgt catagaccga
     1381 tcaagttgag cgcccggaag gcaccgatgc gtcggtcagc gacctttggc agggtcaatg
     1441 cttcggccac tgtcgccgag tcgattccca aatcatcatc caaatatctg aaaatattgc
     1501 agttgtagtt attgcgactc aaagtcataa aacttgacac taacacacta aagttttata
     1561 ttaccgacat cttgatattg actcatttcc aaggaggttc atcaacatgt tgaccgtttc
     1621 acgctgggag cccttccggg acatgctcac acttcagcag cgactgaacc gctttctcgt
     1681 cgacccgtcg aacgcctacg gccccgaagg ggtcggttcc tgggctcctc cggtcgacat
     1741 cgtcgaagag ccggagcgcc tgctcttccg cgccgagatc cccggggtgt cgaaggatga
     1801 catcgacatc aaggtggaga acggcaccct gatcctcagg ggggagaaaa agcaggaact
     1861 cgaggtcgag ggggagacgt cccaccgtgt cgagcgcttc tacggcgcct tcacccgcag
     1921 cttcgcgctg ccggcgacca tcgacgcgga caagatccag gcccgctaca aggacggcgt
     1981 cctggaactc gtcctgccga aggccgagat cgccaagccg cgcaaaatcc agatcgtcgc
     2041 cgcgtaagag cctccgcgcc cgccgcgcgc gggggcatgg ggcgccgggc cgccggtc
//
LOCUS       DASRIQ010000099         5116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2377291, whole genome shotgun sequence.
ACCESSION   DASRIQ010000099 DASRIQ010000000
VERSION     DASRIQ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377291"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /gene="dnaA"
                     /locus_tag="VFD06_02405"
     CDS             complement(<1..1123)
                     /gene="dnaA"
                     /locus_tag="VFD06_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZN02417.1"
                     /translation="MTLWDRILTSLQQKMNAQSFNTWLRPTHQLSLAEGLLQVEVPSS
                     LFVEWIDRNYRPLIQESAIECDAGNLQVHFTSRDLPAPPRTAPARDGLMSRILPGRRM
                     GPAPSDGHAEVRPIGAATMIDGPMAPPVRPSLPAAATLPGSGLTPRYTFDTFVVSSCN
                     QFAHAASVAVAEQPSGAYNPLFIYGGVGLGKTHLMHAVGNRITRSLPGLRMLYISAET
                     FMTELINSIRYERTIDFKERYRGVDVLLIDDIQFLAGKERTQEEFFHTFNTLHNANKQ
                     IVITSDRSPRQLATLEDRLRTRFEWGLLADIQPPDLETRIAILQLKAIRDRLVVPDDV
                     QHFIASKFESNIRELEGALLRVAAFASLSRSAIDLPLVER"
     gene            1990..2124
                     /gene="rpmH"
                     /locus_tag="VFD06_02410"
     CDS             1990..2124
                     /gene="rpmH"
                     /locus_tag="VFD06_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZN02418.1"
                     /translation="MKRTFQPNNRKRRKVHGFRSRMSTKNGRLVLKRRRAKGRKRLTV
                     "
     gene            2121..2474
                     /gene="rnpA"
                     /locus_tag="VFD06_02415"
     CDS             2121..2474
                     /gene="rnpA"
                     /locus_tag="VFD06_02415"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZN02419.1"
                     /translation="MTAGDERFRPEDRIRKRSEYQALYESARRIPSKSFVLFVQKNAC
                     GRPRLGITVSRRIGGAVQRNRAKRLVREIFRRHKRELQDVDIVVNGRASLPASDYHRL
                     EAELLGCLRPYRRTA"
     gene            2474..2707
                     /gene="yidD"
                     /locus_tag="VFD06_02420"
     CDS             2474..2707
                     /gene="yidD"
                     /locus_tag="VFD06_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZN02420.1"
                     /translation="MARAAGHRLDAGAIALIDLYQRLVSPLLPRACRFHPTCSEFCAE
                     AIRRHGLARGLARGALRLLRCQPFSRGGYDPVR"
     gene            2707..4464
                     /gene="yidC"
                     /locus_tag="VFD06_02425"
     CDS             2707..4464
                     /gene="yidC"
                     /locus_tag="VFD06_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZN02421.1"
                     /translation="MEKRLALAVALCIGFLVLWTWFMDRTSPRPPAPAVESGVPAGEP
                     AGAAGTTEAAPGATGEAVGTGPGAVPAPAGVSPAPAPAAEAVAASAEEEILLDLPYAT
                     VRLTNRGGRVTSWTLKGFRDHRGQPLDLVSKDAAKLDRLPLDLLVDDATVAGRLRTGL
                     YRVEREESAEGAAPTTTIRFTWSDGAGTGATKILRTGGASGLVEIEASATANGRPVDP
                     DIAWGAGFEQDRGEVSETMGVGVRALRSRSGSIERRFQSKITLGEPWIEEGSIDFAGV
                     ESKYFAAIFVPTPGAPVRAKTETMRLVEEGREHFHLAFSIRPPAGAAVRLFAGPKDWE
                     VLQAADHGLDRLLDFGFFGVVAHPLFAALKFVHRYVNNWGWAIVLLTVAIRLAFFPFL
                     HRSQLKMRQMQEKMKRIQPKLKSLKERYHRLEKKEVEKGGAGGRHRVRQQQNEEMMKL
                     YQEEGINPLSSVSGCLPLLAQMPILAAFYTILSISIELRHAPWVLWVHDLSEKDYRLV
                     FLMSASMLAQQLMTASAIPDPAQRRMMYLMPIIFTFFFINMPSGLVLYWLVNNLLGIL
                     QQYLVNKEADAQARAAAAA"
     gene            4494..5036
                     /locus_tag="VFD06_02430"
     CDS             4494..5036
                     /locus_tag="VFD06_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HZN02422.1"
                     /translation="MKPGDEMRRDADRGLAEEEAPPADPKDVETLANELLHGLGLELD
                     ASARDTGTTIEVDLSGPDRDLMLDHRGEALNALQYLLNRVVYRGRIGRKIHLDSEGYR
                     RSREDEIVEVARRTAEGVLAKGQESLLSPLNPYERRLVHLALGEIDGIGTRSVGDGFL
                     KRIAIFRTTAKGPRPGPAEG"
BASE COUNT          834 a         1732 c         1736 g          814 t
ORIGIN      
        1 cccgctccac cagggggagg tcgatcgcgc ttcgggacag cgaggcgaag gccgccaccc
       61 ggagcagcgc gccctccagc tcgcggatgt tgctctcgaa cttcgacgcg atgaagtgct
      121 gcacgtcgtc ggggacgacg agccggtccc ggatcgcctt gagctggagg atggcgatcc
      181 tggtctccag gtccgggggc tggatgtcgg ccagcaggcc ccactcgaac ctggtacgca
      241 ggcgatcctc cagcgtcgcc agctggcgcg gcgaacggtc tgaggtgatg acgatctgct
      301 tgttggcgtt gtgcagggtg ttgaaggtgt ggaagaactc ctcctgggtc cgttccttgc
      361 cggcgaggaa ctggatgtcg tcgatgagca ggacgtccac gccgcggtag cgctccttga
      421 agtcgatcgt cctctcgtag cggatcgagt tgatgagctc ggtcatgaag gtctcggcgc
      481 tgatgtagag catgcgcagc cccggcaagg agcgggtgat gcggttgccg acggcgtgca
      541 tcaggtgggt cttcccgagc cctaccccgc cgtagatgaa gagggggttg taggccccgg
      601 aaggctgctc cgcgaccgcc accgaggcgg cgtgcgcgaa ctggttgcag gaggacacca
      661 cgaaggtgtc gaaggtgtag cgcggcgtca gcccgctgcc cggcagggtc gcggccgctg
      721 gcagcgacgg cctcaccgga ggcgccatcg gcccgtcgat catcgtcgcc gcgccgatcg
      781 gccggacctc ggcgtggccg tccgacggcg cgggacccat ccgccgcccc ggcaggatgc
      841 gactcatcag tccgtcgcgc gcgggggcgg tgcgcggcgg cgccggcaga tcccggctgg
      901 tgaaatggac ctggaggttc ccggcgtcgc actcgatcgc gctttcctgg atgaggggcc
      961 ggtagttccg gtcgatccac tcgacgaaga ggctgctcgg cacttcgacc tgaagcagtc
     1021 cttcggcgag cgagagctgg tgggtcggcc gcagccacgt gttgaacgat tgcgcgttca
     1081 tcttctgctg cagcgacgtc aaaatccgat cccagagggt catgttacct cgtccgaaat
     1141 tcggttttgt ttcccctgcg ctgctgcgat ccgctcccaa cgggccttcc gccacgacag
     1201 cccgcacgtc cttcaacagc ttttccacag gtgtggaaaa atcctgacct tgcaaacgct
     1261 tgaagggaag gaacttcggg gggcgtcggg gacgggcggc agcgatctta tgtcaacctg
     1321 tcgagcgccc actacgtatg gggccggtcc cgccgttcgg ccaaccttta gggcctcacc
     1381 gcgtgcggac acccgcctcg cgtcgttcag agacaccacg accccccccg gaaccacttc
     1441 cggaaaaacg tccgacccgt gcactgcttc ggtgactgtg tactgtgttg tgaatggtcg
     1501 gcggaatgta ccaccatcga aatgctcgcg cgcgatcgaa attcgaaaaa aaattcttgc
     1561 cgcaccggtt gctcgaaaaa aacgcagccg gcagggtacg gggtccggtg gcgctggcct
     1621 tcggcgcgcc acctccccct cgatcgcgca tcgcgcctgc cgactgagtt tttttcgagc
     1681 gtccgattac gaaacacttt tcccgatttc gatcacgcgc atcgtctcca cgcttggaat
     1741 gttttctcgg cgtgcgtcct ctcgagtcgc ggttctggat ccggatcgga ggagcggcag
     1801 gcgcgatgcg cgatcgaggg ggaggtggcg cgccgaaggc cagcgccgcc ggaccccgta
     1861 ccaggcgggc tttgcgcctg ccgctccccc ggtgcatatg gggagccgtg ccttgacagt
     1921 aagaatgcag gagaaccata atccgcgctt cctgaagagc atacgcgcga gcgaaggaga
     1981 agaccgttca tgaaacgcac gttccagccg aacaaccgga agcggcgcaa ggtgcacggt
     2041 ttccggtccc ggatgagcac caagaacggg cgcctggtac tgaagcgccg ccgggccaag
     2101 ggacgtaagc gcctgacggt gtgaccgccg gcgacgagcg gttccgtccc gaggacagga
     2161 tccgcaagcg cagcgaatac caggctctct acgaatcggc ccgtcgcatt ccctcgaaga
     2221 gcttcgtcct ctttgtgcag aaaaacgcct gcggacggcc gcgtctcggc atcaccgtct
     2281 cccgccgtat cggcggcgcg gtgcagcgga accgcgccaa acggctggtg cgcgagatct
     2341 tccgccgcca caagcgggag ctccaggacg tggacatcgt ggtgaacggc cgcgcgtcgc
     2401 tccccgccag cgactaccac cggctcgagg cggagctgct cggctgcctg cgtccgtatc
     2461 ggaggaccgc ctgatggcgc gcgcggccgg ccatcgcctc gacgccgggg cgattgcgct
     2521 gatcgacctc tatcagcgcc tggtctcccc gctcctgccg cgggcgtgcc gcttccaccc
     2581 gacctgctcg gagttctgcg ccgaagcgat ccgccggcac gggctggcgc gcgggctcgc
     2641 gcgcggcgcg ctccgcctcc tgcgctgcca gccgttcagc cgcggcggct acgacccggt
     2701 ccgctgatgg aaaaacgact ggcgctcgcc gtggcgctgt gcatcggctt cctggttctc
     2761 tggacatggt tcatggaccg gacgtcgccg cggcctcccg cccccgccgt ggagagcggc
     2821 gtccccgcag gtgaaccggc cggggccgcg ggcacgaccg aggcggcgcc tggcgccacc
     2881 ggtgaggcgg ttggaacggg acccggcgcc gtccccgcgc ccgcgggcgt gagtccggcg
     2941 cccgccccgg cggccgaggc ggtcgccgcc tccgccgagg aggagatcct cctcgacctg
     3001 ccgtacgcga ccgtccgcct cacgaaccgc ggcgggcgcg tgacctcctg gaccctcaag
     3061 ggctttcgcg atcaccgcgg ccagccgctc gatctcgtct cgaaggacgc ggccaagctg
     3121 gaccgcctgc cgctcgacct gctggtggac gacgcgacgg tcgccgggcg gctgcgcacc
     3181 ggcctgtacc gggtggaacg ggaggagagc gccgaagggg ccgcaccgac gacgacgatc
     3241 cgcttcacgt ggagcgacgg agcgggaacc ggggcgacga agattctccg gaccggcggc
     3301 gcatcgggac tcgtcgagat cgaagcctcc gcgacggcca acggccgccc tgtcgacccg
     3361 gacatcgcct ggggggcggg gttcgagcag gaccggggcg aggtctccga gacgatgggg
     3421 gtcggcgtcc gcgccctccg cagccgctcc ggatcgatcg agcggcgctt ccagtcgaag
     3481 atcacgctcg gcgagccctg gatcgaggaa ggttcgatcg acttcgcggg ggtcgagagc
     3541 aaatacttcg ccgcgatctt cgtgccgacg cccggcgcgc cggtccgcgc gaagaccgag
     3601 acgatgcgcc tcgtcgagga ggggcgcgag cacttccatc tcgccttctc gatccgcccg
     3661 ccggccgggg ccgcggtgcg gctgttcgcc gggcctaagg actgggaggt gctgcaggcg
     3721 gccgaccacg gtctcgaccg cctgctcgac ttcggcttct tcggcgtcgt cgcccacccg
     3781 ctgttcgcgg cgctcaagtt cgtgcaccgc tacgtcaaca actggggctg ggcgatcgtc
     3841 ctgctcaccg tcgccatccg cctggcgttc ttcccgttcc tgcaccgaag ccagctcaag
     3901 atgcggcaga tgcaggagaa gatgaagcgc atccagccga agctgaagtc gctcaaggag
     3961 cgctaccacc ggctggagaa gaaggaggtc gagaaggggg gagccggcgg gcggcaccgg
     4021 gtacggcagc agcagaacga ggagatgatg aagctctacc aggaggaggg gatcaatccc
     4081 ctctcgtccg tctccggctg cctcccgctc ctggcgcaga tgccgatcct cgcggccttc
     4141 tacaccatcc tgtcgatcag catcgagctg cgccatgcgc cgtgggtgct gtgggtgcac
     4201 gatctgtcgg agaaggacta tcgcctcgtc ttcctcatga gcgcctcgat gctggctcag
     4261 cagctcatga ccgcgtcggc gatcccggat cctgcgcagc gtcggatgat gtacctgatg
     4321 ccgattatct tcacgttctt cttcatcaat atgccttcgg ggctggtgct atactggctc
     4381 gtcaacaatc tcctcggaat cttgcagcag taccttgtca acaaggaagc cgacgcgcag
     4441 gcgcgtgcgg cggcggccgc ctgaatcggc gacgcgcggg ggccacgatg tcgatgaagc
     4501 cgggggacga gatgcgacgc gacgccgatc gtgggcttgc cgaggaggaa gcgccgcccg
     4561 ccgatccgaa agacgtcgaa acgctggcca acgagctgct gcacggcctc gggctcgagc
     4621 tcgacgccag cgcgcgcgac accggcacca ccatcgaggt cgatctgagc ggtcccgacc
     4681 gcgacctgat gctcgaccac cggggagagg cgctcaacgc cctgcagtac ctgctcaacc
     4741 gcgtcgtcta ccgcggccgc atcggcagga agatccacct cgacagcgaa ggataccggc
     4801 gctcccgcga ggatgaaatc gtcgaggtcg cccggcgcac cgccgagggg gtcctggcaa
     4861 aaggacagga gtcgctcctg agccccctca atccctacga gcgccgtctc gtgcacctcg
     4921 ccctggggga gatcgacggg atcggcacgc gcagcgtcgg cgacggattc ctcaagcgca
     4981 tcgcgatctt ccgcaccacg gccaaaggac cccggcccgg gccggccgag ggctaatctc
     5041 cggagtccgc atgcggggtc cggcggcatc gtgagcctcc cgccgcgcga agacaccatc
     5101 gtcgccatcg ccacgc
//
LOCUS       DASRIQ010000100         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_811083, whole genome shotgun sequence.
ACCESSION   DASRIQ010000100 DASRIQ010000000
VERSION     DASRIQ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811083"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1198)
                     /locus_tag="VFD06_02435"
     CDS             complement(80..1198)
                     /locus_tag="VFD06_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZN02423.1"
                     /translation="MRWPRRLTAVQQVGQLLWIGFEGTTFTPPLARLLRDVQPGGIIL
                     FGRNLPHEARRLRALTDALVRAVAIPPFIALDQEGGRVSRLRGLLGPTLTGAALGARR
                     DAPSAVRRHAEGTAMTLRTLGFNVNFAPVLDLSRAEADNGIGDRAYGDDPLTVSRLAG
                     IFAATHTRAGVVPVGKHFPGLGPAVGDTHAVLPVVRTPRAALLRRDLLPYRKLRRALP
                     MVMIGHACYPGIQDRDDQPASLSPAVVTDLLRGTLDYRGLVLTDDLEMGAIDQTLGAG
                     ALAVAAFRAGSDGLMFCKSEDRIRDAAATLLVAARDGRIPADRLRGSLRRIAALKRRA
                     LVARRRLRFTAGSLARAKAVLADLGPAPVFGPDPTART"
     gene            1580..2482
                     /locus_tag="VFD06_02440"
     CDS             1580..2482
                     /locus_tag="VFD06_02440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN02424.1"
                     /translation="MAKKFYTIMIVPHATAKFRRLRVSKNLLLGGGAFLSALLLAGLT
                     VPHFLIRTLSLSHGLAKVTQENAELRKSNERFDASLNELRNRLQEIESKATKFALMVG
                     VENLPTQTMGAGGGTLPDLRGIDPRQAEPLIDSQIRTLKERSGILQDSFQVLDTAFQK
                     QALLLSSTPSIYPVRGLLGNGFGWRRDPFTGMRDFHQGLDIIAPQGTRVVAPADGIVT
                     RVGPAGGFGLSLFISHGYGLVTRYAHLSSTSVKVGQRVRRGEVVASTGSTGRSTGPHL
                     HYEVMVHQKAVDPLVYILEESKVF"
BASE COUNT          424 a          934 c          957 g          382 t
ORIGIN      
        1 ctaccgcgac atcttccgcc gcctcgccga ccgcgatctc gagtaaggcg cagcccgcgt
       61 tcggggctgt gtcgccgcgc taggtgcgcg cggtggggtc ggggccgaag acaggggccg
      121 gcccgaggtc cgcgagcacg gccttcgccc gggcgaggct cccggccgtg aaccggaggc
      181 ggcgccgcgc cacgagcgcc cgccgcttga gggcggcgat gcgacgcagc gagccgcgca
      241 gacgatcggc cggaatgcgg ccgtcgcgcg ccgccacgag gagcgtggcg gcggcgtcgc
      301 ggatgcgatc ctccgacttg cagaacatca gcccgtccga cccggcgcga aacgccgcca
      361 cggcgagcgc cccggccccc agcgtctgat cgatcgcccc catctccaga tcgtcggtca
      421 gcacgaggcc gcggtagtcg agcgttccgc gcaggaggtc cgtgacgacc gccggcgaca
      481 gactcgcggg ctggtcgtcc cggtcctgga tccccgggta gcaggcgtgg ccgatcatca
      541 ccatcggcag cgcccggcgc agcttccggt aggggaggag atcgcgtcgc aggagcgccg
      601 cccgcggcgt ccggacgacc ggcagcacgg cgtgcgtgtc gccgaccgcc ggccccagtc
      661 cggggaaatg cttgccgacc gggacgactc cggcccgggt gtgggtcgcg gcgaagattc
      721 cggccaggcg cgacacggtc agagggtcgt cgccgtaggc gcggtccccg atgccgttgt
      781 ccgcctcggc gcgcgacagg tccaggaccg gcgcgaagtt gacgttgaac cctagggtcc
      841 ggagcgtcat ggcggtcccc tcggcgtggc ggcggacggc gctgggtgcg tcgcggcgcg
      901 ccccgagcgc cgcaccggtc agggtcggcc cgagaagccc gcgcagccgg ctgacgcggc
      961 ctccctcctg atcgagcgcg atgaacggcg ggatcgcgac cgcccggacg agcgcatcgg
     1021 tcagcgcccg caggcgccgc gcctcatgtg ggaggttgcg gccgaacagg atgatgccgc
     1081 cgggctgaac gtcacgaagc aggcgggcga gcggcggggt gaaggtcgtc ccctcgaatc
     1141 cgatccacag gagctggccg acctgctgga cggcggtcag gcgtcgaggc cacctcatgg
     1201 gaggggggct ccgttgcggc gagaggacgg gtgcattcgg ccgggagaat ctaagcgatg
     1261 ccccgaagcg atcgcaaggc gccgctccgg ggcggacgaa ccggtgtcgg gccagtggcg
     1321 ttcaacagtt cgaatctcca attcgtcatc ggaccttgac ggtgtaacgc gccgaaccta
     1381 cattggatgt tgactatgtg atttttgtat ttctgatttg tattcagagg taatggccaa
     1441 tggagccccg catgttgtgt ctcgaagcgc cgcgagcccc gtatctgggg tggaggggag
     1501 gcgagtcgcg tgttgactcc ctccaggtcg ggtgatagca tcccgcgctt caaacccgcg
     1561 gcgggccggg ggacccaggg tggccaagaa gttctacacc atcatgatcg tcccgcatgc
     1621 gacggcgaag ttccgccgcc tgcgtgtgtc gaagaacctc cttctggggg ggggcgcgtt
     1681 cctgtccgcc ctgctcctcg ccggcctgac ggtgccccac ttcctcatcc gcaccctgag
     1741 cctgagccac ggcctcgcga aggtgaccca ggagaacgcc gagctccgca agagcaacga
     1801 gcggttcgac gcgtcgctca acgagctgcg caaccgcctc caggaaattg aatccaaggc
     1861 gacgaagttc gccctgatgg tcggcgtcga gaacctgccg acccagacga tgggggcggg
     1921 cggcggcacg ctgccggacc tgcgcggcat cgacccgcgt caggcggagc cgctcatcga
     1981 ctcgcagatc cggacgctca aggagcgctc ggggatcctg caggactcct tccaggtgct
     2041 cgacaccgcc ttccagaagc aggcgctcct gctctcctcg acgccgagca tctacccggt
     2101 gcgcggcctc ctcgggaacg gcttcgggtg gcgccgcgac cccttcaccg gcatgcgcga
     2161 cttccaccag gggctcgaca tcatcgcgcc gcaggggacg cgcgtcgtgg cccccgccga
     2221 cggcatcgtc acccgggtcg gcccggccgg agggttcggg ctgtcgctgt tcatctcgca
     2281 cgggtacggc ctggtcaccc gctacgcgca cctgagctcg acctcggtca aggtcgggca
     2341 gagggtccgg cgcggcgagg tggtcgcctc gaccggttcg accggccgca gcaccgggcc
     2401 gcacctgcac tacgaggtga tggtccacca gaaggcggtc gacccgctgg tctacatcct
     2461 ggaagaatcg aaggtcttct aaagccccga gaacccggac cgagcgcgcg ccgcccgcaa
     2521 acgggcggcc gaagtcgttt atcataagcc gatgatcatc gacaccatcc tcaaggcgct
     2581 gttcgggacg aagaacgagc gcgagctcaa gaagatcgtc ccgcgcgtcg ccgccatcaa
     2641 cgccctcgag gcggaggtgg cccccctgag cgacgccgcc atccgcgagc gggtcga
//
LOCUS       DASRIQ010000101         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_559208, whole genome shotgun sequence.
ACCESSION   DASRIQ010000101 DASRIQ010000000
VERSION     DASRIQ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559208"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1459
                     /locus_tag="VFD06_02445"
     CDS             293..1459
                     /locus_tag="VFD06_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZN02425.1"
                     /translation="MTEHRSARLEPGEEFVVPLAVDARGREALGVHQVLIRVRAGWAG
                     REGATEYGALAVMGAAPGAEVVLDGGMAGHAGADGSLRLASVPVGQREVRLRGSSGSV
                     VSRTVVIVKGRTVQVSPEAADGGSASQPSLSATGKNAEGFQEYRRVRDGATMVEIPEG
                     EFLMGNLETEGSPQPHTVFVSTFLMDKLPMTVGRYRRFAMATGRPLPPDPYWGVHEDH
                     PVAFVRWDEAKAYCEWVEGRLPTEAEREKAARGTDGRMFPWGSEPPSDKRAVFGRFWG
                     ELGNDTVGIRPAGAGPYGLLDTSGNMWEYCEDWYSPDDYASSPKKDPAGPKTGRARVV
                     RGSSWDSRWVTLSASKRNFAYTGYREGDFGFRCAANPPARHESSASTLPSGEGR"
     gene            1456..3033
                     /locus_tag="VFD06_02450"
     CDS             1456..3033
                     /locus_tag="VFD06_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZN02426.1"
                     /translation="MTRRLILLVVLAAASVLAARGAPRAEAVWTRLELVENSAEGPAY
                     TRCQLEATPRKGQGAGWRLFYGEARVDAASGWSAIRSSSGCRPSESEPAEMTWGLTGS
                     PYFVVDISLGPASVSGREIHMEATFSIKKLTGFAPDGSPTYEPSIEKRTVRVPEGSSA
                     VVPILVASVKETDEFRVRELLLKFRAHVSGAGPRVEYGELAVTADVPRAEIFLDGGPV
                     GRTSAEGAVILTAVRTGEREVVVRDASGREARTVTRVGKGRRTAVALTLLKGAPASTE
                     GLRPLGPNPQGGEEFWRDKDGAIVVRIPGGEFQMGSAEAEGEASERPRHAVRVHGFLM
                     DKTEVTWGQYRRFQSASGRSVAKPPIWGTPEALPVSGVSWDDARAFCEWVGGRLPTEA
                     EWERAARGDDARTYPWGGTFAPWRCNTRDGGPHAPTPAGAYPDCVSEHGVLDLAGSVA
                     EWCSDWYKSGYDVADGLVENPRGPESGTMRSVRGGNWMSSSFGVRGASRLGVEPAWNG
                     PMQGFRCVQEIRTTDGR"
BASE COUNT          469 a          939 c         1194 g          487 t
ORIGIN      
        1 ggtatcgttt gccttctggg gcacatccgg cgagggtgca cccggccggt gcggagccgg
       61 gaccttcacg gacaagtccc cggaccccac cgcggctcct tccggtgcga ccgagacgcc
      121 ttcgggcgcg gcccgggcga cattcctgat ccatgaggtc tctgcggatg tcggatggga
      181 cggaggggcc atggaccccg gtcctctggg agcgttccag atctttctga cgctgcgaac
      241 gcggcagttg accggtttgt cgaaggaggg ccggccgcag tacggagctg ccgtgaccga
      301 acaccggtcc gcccgtctcg agccggggga agagttcgtc gtgccgctcg cggtggatgc
      361 acgcggacgc gaggctctgg gggtccatca agtgctgatc cgggtccggg ccggctgggc
      421 gggtcgcgag ggggcgaccg aatacggcgc actggctgtg atgggagcgg ccccgggagc
      481 cgaggtggtg ctggacggtg ggatggccgg ccacgccggg gccgacggga gcctgcggct
      541 cgcgagcgtg ccggtcggac aacgggaggt gcgcctgcga ggttcatcgg ggtcggtggt
      601 ctcgcggacc gtggtgatcg tgaaggggag aacggtgcag gtgtcgcccg aggccgcgga
      661 cggcggctcg gcatcgcagc cgtcactttc ggccacgggg aagaacgcgg aagggttcca
      721 ggagtaccgg cgggtgcggg acggggcgac gatggtggag atcccggaag gggagttcct
      781 gatggggaac ctcgagaccg aggggtcgcc gcagccgcac acggtgttcg tctcgacgtt
      841 tctgatggac aagctgccga tgacggtggg acggtacagg cggtttgcga tggcgacggg
      901 gcgaccgttg ccgccggatc cgtactgggg cgtgcacgag gatcatccgg tggcgttcgt
      961 gaggtgggac gaggcgaagg cgtattgcga gtgggtggag ggccgtctgc cgacggaggc
     1021 ggaacgggag aaagcggcgc ggggaaccga cggtcgcatg ttcccctggg gcagcgagcc
     1081 gccgagcgac aagcgtgccg tcttcgggcg cttctggggt gagctgggga acgacacggt
     1141 cggaatccgg ccggcgggcg ccggtccgta tggtctgctc gacacgagcg gcaacatgtg
     1201 ggagtactgc gaggactggt acagtcccga tgactacgcg tcgagcccga agaaggaccc
     1261 cgcaggtccg aagaccggtc gggcacgcgt cgtccgggga tcctcgtggg acagtcgctg
     1321 ggtgacgctg agcgcttcga aacggaattt cgcctacacg ggctaccgcg aaggggactt
     1381 cggatttcgc tgcgctgcga atcctccggc gcgtcacgaa tcgtccgcga gcacgttgcc
     1441 ttctggtgag ggtcgatgac caggcgcctg atccttctgg tggttctcgc tgcggcctcc
     1501 gtgctcgcgg cccgcggtgc tccccgcgcc gaggccgtgt ggacgcgtct cgagctcgtc
     1561 gagaacagcg cggaaggacc tgcgtacacc cgctgtcagc tcgaggcgac gccgcggaaa
     1621 ggccagggcg caggctggcg gctcttctac ggcgaggcgc gcgtcgacgc tgcctccgga
     1681 tggtccgcca tccgctcctc gagcgggtgc aggccatccg aatccgagcc ggcggagatg
     1741 acgtggggat tgaccggatc gccctacttc gtcgtggaca tatcgctcgg gccggcgagc
     1801 gtctccggcc gcgagatcca catggaggcc accttctcga tcaagaagct gaccggcttc
     1861 gcgccggacg gcagtccgac ctacgagccg agcatcgaga agagaacggt tcgtgtgccg
     1921 gaggggagca gtgcggtggt tccgattctg gtcgcgagcg tgaaggaaac ggacgagttc
     1981 agggtccgcg aactgctcct gaagtttcgc gcccacgttt ccggcgccgg gccgcgggtc
     2041 gaatacggcg agttggccgt cacggccgat gttccgcgcg ccgagatctt cctggacggc
     2101 ggtccggtgg ggcggacatc cgccgagggg gcggtcattc tcaccgccgt gcggaccggc
     2161 gagcgggagg tggtcgtccg ggacgcctcc ggacgcgagg cgcgcaccgt gacgcgggtc
     2221 ggcaaagggc gccggaccgc tgttgcgctg acccttctga agggcgcccc tgcgtccacg
     2281 gaggggctgc gtccgctcgg gcccaacccg caggggggcg aggagttctg gcgcgacaag
     2341 gacggcgcca tcgtcgtgcg cattccgggc ggcgaattcc agatgggaag cgccgaggcc
     2401 gaaggggagg cttccgagcg tccgcggcac gccgtccgcg tccacggctt cctcatggac
     2461 aagaccgagg tgacgtgggg ccagtaccgg cgcttccagt ccgcgtcggg caggtctgtc
     2521 gccaagcctc ccatctgggg aacacccgaa gccctgccgg tatccggcgt gtcctgggac
     2581 gatgcccggg ccttctgtga gtgggtgggg ggccgcctcc cgaccgaggc cgaatgggag
     2641 cgggcggccc gcggcgatga cgcacggacc tacccatggg gcggaacgtt cgctccctgg
     2701 cgctgtaata cgagggacgg cggtccccat gccccgactc cggcgggagc gtacccggat
     2761 tgcgtcagtg agcacggcgt tctcgatctc gcgggcagcg tcgcagagtg gtgttcggac
     2821 tggtacaaga gcgggtacga cgtcgccgat ggtctggtcg aaaaccccag gggacccgaa
     2881 tcaggaacga tgcggtcggt ccggggaggg aactggatga gttcctcctt cggcgtccgc
     2941 ggcgcatccc gactgggagt cgaaccagcg tggaacggcc ccatgcaggg gttccggtgc
     3001 gttcaggaga ttcggacgac ggacggacga tgatcgcgat ccttttcccc ttgttggcgg
     3061 ccgtcgtgac gcttcctgcg aacgacctt
//
LOCUS       DASRIQ010000102         6792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1093358, whole genome shotgun sequence.
ACCESSION   DASRIQ010000102 DASRIQ010000000
VERSION     DASRIQ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093358"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFD06_02455"
     CDS             complement(<1..872)
                     /locus_tag="VFD06_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN02427.1"
                     /translation="MQAPHVVSVLAEIAATASETLELQEVFDRVAAAIRRLIPCDHVG
                     VVRILDGDWAVMHATTFDCGESGGKCSDPFPLGEWSPRMRPRPGAIPRVDDAQSELDP
                     SFTADAGILASGVRSALWEPFRLGASWTGGIWLTSPEAGAFTEEHQKMLAPIATLLGS
                     AVEHWRIWDAERRRRERLDRLEAPLGALQETLDVREVFTRLSQSVQEVLPHQFLVLVE
                     IDEAARSFKVLARAGDVDVAVPDGPIVFSQVELDRRTLDFEIHRDIAAEMAGDTPRNR
                     LMIASGMRSWLRVPV"
     gene            1078..3411
                     /locus_tag="VFD06_02460"
     CDS             1078..3411
                     /locus_tag="VFD06_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HZN02428.1"
                     /translation="MPPLPVPHGPARAVLLPVSPKTLFLYWVLDEVVRARLADASGPA
                     EIRLEAPTGGSPEGSGAVEALSVPFDFRSGSWYLTLPGLKGRVRARLGLFERGRFVEV
                     LASNEIPIPRTEPGSDPEVWMDRRALRAGRGQPLPPPTTRRLERSARVGPYAAGAGEI
                     GRPDAERVRRATSPAGRVAWSEATHQRAAARSPEPGADALVTWTEILAANDALAGRRP
                     PGKVAVPPLGLANPAGYLCFMLHAHLPFVRHPEHDFFLEEQWLFEGITETYLPILDNL
                     DALARERVPVRLTMSLSPTLLAMLSDPLLMQKYERLLGRLCHLAWREVERTRKDPDFS
                     PIAGFYFDRLHRYQHLFVDVWNRDLLGVFRRLEDQGVLELLTCCSTHGYLPLHRLDPG
                     TLRGQIDTAVAEHRRLLGRSPRGLWLPECAYFEGLDAYVADAGIEYFFVDTHGIRNAT
                     SPPRFDVHAPLLCPTGVAAFGRDEESSVQVWSSQEGYPGDPSYRDFYRDIGYDLEHTY
                     VAPYLDPAGTRGMTGFKYHRITGRTDWKEPYRRHAALRTAARHAADFVRNRRAQIDWL
                     KGSLDRPPLVVSMYDAELFGHWWFEGPDWLDLVLRGLPAAGIAAISPAQYLEAHPVTQ
                     VARPSESSWGENGYHDVWLRGDNDWILPPLHDAGRRMAGIAQELARDGRVGDRASSGA
                     DLRRRLAAQAARELLLAQASDWPFILKNRTTPEYARRRVHEHLERFERLARLFASRGI
                     RDGADDADTRRAEEALAAIASTDNLFPEIDPGLWGER"
     gene            3408..4697
                     /gene="glgC"
                     /locus_tag="VFD06_02465"
     CDS             3408..4697
                     /gene="glgC"
                     /locus_tag="VFD06_02465"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZN02429.1"
                     /translation="MNGPDALGARGPRPAGGSGRKLQAIVLAGGEGRRLMPLTRRRAK
                     PAMPFAGAYRLVDFVLSNLVHSNMFHIHVLTQYQSYSLIRHLSRGWTFASNLGQYCEV
                     IPAAFGEGKGWYLGSADALYQNLERMERDRPTYVAVLGADHIYRMDYQQMLDEHVESG
                     ADISVSVVPHAIEEAHQFGCLEVDGGMRVTRFLEKPKSPPPFPGDPSKALVSMGNYIF
                     SYEVLREAVLHDHEASDSTHDIGRDILPRWYEKRHVRAYNFMNNRVPGAPDSDMGYWR
                     DVGTLDAYWRASMDLVSVTPSFNTYNYEWPILTARRDDPPAKFVFADRESNRAGIATD
                     SLVCNGGIISGGHIDRCVLSPRVRINSYATVSESVLFEEVDVGRRCRIRRAIIDKNIR
                     IPPDTVIGYDAEQDRARGITVSGEGIAVVSSEARFGG"
     gene            4734..>6792
                     /locus_tag="VFD06_02470"
     CDS             4734..>6792
                     /locus_tag="VFD06_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HZN02430.1"
                     /translation="MPRASIAFLWHLHQPLYRLQGERICRQPWVRLHAARSYYDMVRV
                     LDDFPGVRVTLNLVPTLLEQMRDYVRGGTDTFREAARVPIADGDEAQRRFLVGHFFSA
                     QEERMIRPLPRYADLHRLRADARRRRGVSEAWRDFTLSDLRDLQALFDLAWFGFKAAE
                     DFPEIAALRRRGRGFTREEIETIHRIQDDILRRLPDLYRAAAARGQEISASPYAHPIL
                     PLLADTESALDAMPDAELPERLRAPEDARAQIADGIAAIEAEIGRRPRGMWPSEGSVS
                     AAAVEAMAAGGLVWAASDADVLRASERDGDADPYRPWHLEGAAAPVALLFRDHDLSDR
                     VGFVYSKRDPDEAASELLAEARARTSGDEGAAILVALDGENPWEQYPDAGGAFLRALY
                     GALEKDSEVVPLPVEEAIAACPRRGTIRRLRAGSWIEASFGTWIGGPEKNRAWSLLGE
                     TRRRLQPALEDPDRTPSHRAAWVALRAAEGSDWFWWLDGQFENLYRAEFDALFRGHLR
                     QACEAAGVAPPEGIGWPIIAGRDRDVLRAPSLDPAIDGFESDYFEWYAATPVDGATLA
                     AGGTMQRAGRPVGALRCAVTPRGELSLRLDPPAPAAAPFAGTRLSLRIAVSEGPVLES
                     RLALDADGNLASAEPDGVRARARKILEVAVPARAILAAGPSSARLSIVLEHEGIAVPL
                     RDLE"
BASE COUNT          939 a         2444 c         2453 g          956 t
ORIGIN      
        1 accggaaccc gcagccagga gcgcatgccg ctggcgatca tcagccggtt gcgcggcgtg
       61 tcgccggcca tctccgcggc gatgtcgcgg tggatctcga aatcgagggt gcggcggtcc
      121 agctcgacct gcgagaagac gatcgggccg tccgggacgg cgacgtccac gtcgccggcg
      181 cgcgccagga ccttgaagga gcgggccgcc tcgtcgatct cgaccagcac caggaactgg
      241 tgcggcagga cctcctgcac cgactgcgac aggcgcgtga acacctcgcg cacgtcgagc
      301 gtctcctgga gggccccgag cggcgcctcc agccggtcga gccgctcgcg ccgccggcgc
      361 tccgcgtccc agatgcgcca gtgctccacc gccgagccga gcagcgtcgc gatcggcgcg
      421 agcatcttct ggtgctcttc ggtgaaggcc ccggcctccg gggaggtgag ccagataccg
      481 ccggtccacg acgcgccgag ccggaacggc tcccacaggg ccgaccggac gcccgacgcg
      541 aggatgccgg cgtcggcggt gaacgacggg tcgagctccg actgggcgtc gtcgacgcgc
      601 gggatggcgc cggggcgagg ccgcatccgc ggcgaccatt cgccgagcgg gaacgggtcg
      661 gagcacttcc cccccgactc gccgcagtcg aacgtggtcg cgtgcatcac cgcccagtcg
      721 ccgtcgagga tccgcacgac ccccacgtga tcgcatggaa tgagacgccg gatcgccgcg
      781 gcgacgcggt caaagacctc ttgaagctcg agggtctccg acgcggtcgc ggcgatctcc
      841 gcgaggacgc tgacgacgtg gggggcctgc atgggagaag ccattctagt ccgcggcggg
      901 gaggcgacgc cagcgatgcg gcgaggaccg tcgaggccgg tccgtgccga tggtgcgcat
      961 ttcccgcaat tgcacgaaga cgcaccggcg catagaatcc tccgacctcg aacgacaacc
     1021 tcattccggg gacggtcggc attggccaga cacgcagtcg gatcggaacc cgagccgttg
     1081 cctccgctgc cggtcccgca cggaccggcg cgcgccgtgc tgctgccagt ctcgccgaag
     1141 accctcttcc tttattgggt gctcgacgag gtggtgcggg cgcggctcgc ggacgcctcc
     1201 gggccggcgg agatccggct cgaggcgccc accggcgggt cgccggaggg aagtggcgcc
     1261 gtcgaggcgc tgagcgttcc gttcgatttc cgcagcggct cctggtacct gacgctgccg
     1321 ggcctgaagg ggcgcgtgcg cgcgcggctc gggctgttcg agcgcggtcg gttcgtcgag
     1381 gttctggcga gcaatgagat cccgatccca cgcacggagc cgggatccga ccccgaggtc
     1441 tggatggatc ggcgggcgct gcgcgccggg cgcggccagc cgctgccgcc gccgaccacg
     1501 cgccgcctgg aacgaagcgc cagggtcggt ccgtacgccg ccggcgccgg agagatcgga
     1561 cggccggatg cggagcgcgt ccgtcgcgcc acctcacccg ccgggagggt cgcctggtcg
     1621 gaggcgacgc accagcgagc cgctgcacga agccccgagc ccggcgccga cgcgctcgtc
     1681 acgtggaccg agatcctcgc cgcgaacgac gctctcgcgg ggcgtcgtcc gccgggaaag
     1741 gtggcggtgc cgccgctcgg tctggcgaac ccggccggct atctgtgctt catgctgcac
     1801 gcccatctgc cgttcgtgcg ccatcccgag cacgacttct tcctcgagga gcagtggctc
     1861 ttcgagggga tcaccgagac ctacctgccg atcctcgaca acctcgacgc gctggcgcgc
     1921 gagcgcgttc cggtgcggct gacgatgtca ctcagcccga cgctcctggc gatgctcagc
     1981 gacccgctgc tgatgcagaa gtacgagcgg ctgctgggcc gcctgtgcca cctggcgtgg
     2041 cgtgaggtgg agcgcactcg caaggatccc gacttcagcc cgatcgccgg cttctacttc
     2101 gaccggctgc accgctacca gcacctcttc gtcgacgtct ggaatcgcga cctcctcggc
     2161 gtgttccgcc ggctcgagga ccagggggtg ctggagctcc tcacgtgctg ctcgacgcac
     2221 ggctacctgc cgctgcaccg gctcgacccg gggacgctgc ggggacagat cgacacggcg
     2281 gtcgcggagc accggcggct cctcggccga tcgccgcgcg gcctgtggct gccggaatgc
     2341 gcctatttcg aggggctcga cgcatacgtc gccgacgccg gcatcgagta cttcttcgtg
     2401 gacacgcacg ggatccgcaa cgccacgagc ccgccgcgct tcgacgtgca cgcgccgctc
     2461 ctctgcccga ccggcgtcgc cgcgttcggg cgggacgaag aatcgtcggt gcaggtgtgg
     2521 agcagccagg agggataccc gggggatccg tcgtaccgcg acttctaccg cgacatcggc
     2581 tacgacctcg agcacaccta cgttgcgccg tatctcgatc ccgccgggac gcggggcatg
     2641 accgggttca agtatcatcg catcaccgga cggaccgact ggaaggagcc ttaccgccgc
     2701 cacgcggcgc tgcgcaccgc ggcgcggcac gcggccgatt tcgtgcgcaa ccgccgggcg
     2761 cagatcgatt ggctcaaggg gagcctcgat cgcccgccgc tcgtcgtctc gatgtacgac
     2821 gccgagctgt tcggccactg gtggttcgag ggccccgact ggctcgatct ggtcctgaga
     2881 ggtcttccgg ccgcggggat cgcggcgatc agcccggcgc agtacctgga ggcgcatccg
     2941 gtgacgcagg tggcccgccc gtccgagtcg tcgtgggggg agaacggcta ccacgacgtc
     3001 tggctgcgcg gcgacaacga ctggatcctg cctccgctgc acgacgccgg ccggcgcatg
     3061 gccggcattg cgcaggagct ggcgcgggac ggtcgcgtcg gggaccgcgc ctcgtccgga
     3121 gccgacctgc gccgccgtct cgcagcgcag gcggcgcggg agctcctgct cgcgcaggcg
     3181 tcggactggc ccttcatcct caagaaccgc accacccccg aatacgcccg gcggcgggtg
     3241 cacgagcacc tcgagcggtt cgaacggctg gctcgtctct tcgcgtcgcg ggggatccgt
     3301 gacggcgccg acgacgcgga cacccggcgc gcggaggagg cgctggccgc catcgcgtcc
     3361 acggacaatc tcttcccgga gatcgatccg gggttgtggg gggaacgatg aacggacccg
     3421 acgcgctcgg ggcgcgtggc ccgaggcccg cgggcggctc ggggcgcaag ctgcaggcga
     3481 tcgtgctcgc gggcggggag gggcggcgcc tgatgccgct gacgcgccgg cgggcgaagc
     3541 cggcgatgcc gttcgccggg gcctaccgcc tggtcgactt cgtcctgtcg aatctcgtcc
     3601 actcgaacat gttccacatc catgtgctga cccagtacca gtcgtactcc ctcatccgac
     3661 acctgtcgcg cggctggacc ttcgcctcga acctcgggca gtactgcgag gtgatcccgg
     3721 cggcgttcgg cgaggggaag gggtggtacc tgggctccgc cgacgcgctg taccagaatc
     3781 tcgagcgcat ggagcgggac cgcccaacct acgtcgccgt cctcggcgcc gaccacatct
     3841 accggatgga ctaccagcag atgctcgacg agcacgtcga gagcggcgcc gacatcagcg
     3901 tcagcgtcgt cccgcacgcg atcgaggagg cgcaccagtt cggctgcctc gaggtggacg
     3961 gcggcatgcg ggtgacccgc ttcctggaga agcccaagag cccgccgccg ttccccggcg
     4021 acccgtcgaa ggcgctcgtg tcgatgggga actacatctt cagctacgag gtcctgcgcg
     4081 aggcggtgct gcacgatcac gaggcgtccg actccacgca cgacatcgga cgcgacatcc
     4141 tgccgcgctg gtacgagaag cggcacgtgc gcgcctacaa cttcatgaac aaccgggtcc
     4201 cgggggcccc ggactccgac atgggctact ggcgggacgt gggaacgctc gacgcgtact
     4261 ggcgcgcctc gatggacctc gtcagcgtca cgccgtcgtt caacacctac aactacgagt
     4321 ggcccatcct gacggcgcgg cgcgacgatc cgccggcgaa gttcgtcttc gccgaccggg
     4381 agtccaaccg ggccgggatc gccaccgact cgctcgtctg caacggcggg atcatctcgg
     4441 gcggccacat cgaccgctgc gtcctgagcc cgcgggtgcg catcaactcc tacgccaccg
     4501 tctcggagag cgtcctcttc gaagaggtgg atgtcggccg ccgctgtcgc atccggcgcg
     4561 ccatcatcga caagaacatc cgcatccccc ccgacaccgt catcggctac gacgccgagc
     4621 aggaccgggc gcgcggcatc accgtcagcg gcgaagggat cgccgtcgtc tcgtccgagg
     4681 cgcgcttcgg cggctgatcg tccgccgccg cgcctccgga gtccagaagc ctcgtgcccc
     4741 gcgcctcgat cgccttcctc tggcacctgc accagccgct ctaccgcctg cagggggagc
     4801 ggatctgccg ccagccgtgg gtgcgcctgc acgcggcgcg gtcgtactac gacatggtga
     4861 gggtgctcga tgacttcccc ggcgtgcggg tgacgctcaa cctcgtcccg acgctcctcg
     4921 agcagatgcg ggattacgtg cgcggcggca ccgacacctt ccgcgaggcg gctcgggtgc
     4981 cgatcgccga cggggacgag gcgcagcgcc gcttcctcgt cggtcatttc ttttcggcgc
     5041 aggaggagcg gatgatccgc ccgctgccgc gctacgccga tctccaccgg ctgcgcgccg
     5101 acgcgcggcg gcgccgcggc gtctcggagg cgtggcggga cttcaccctt tccgacctgc
     5161 gcgacctgca ggcgttgttc gatctcgcct ggttcgggtt caaggcggcg gaggacttcc
     5221 cggagatcgc tgcgctgagg cgccgcgggc gcggcttcac gcgcgaagag atcgagacga
     5281 tccaccggat ccaggacgac atcctgaggc gtctgcccga tctctaccgg gccgcggcgg
     5341 cgcgcggcca ggagatctcg gcgtctccct atgcccatcc gatcctcccg ctcctcgccg
     5401 acaccgagtc ggcgctcgac gcgatgccgg atgcggagct gccggagcga ctgcgggccc
     5461 ccgaggacgc ccgggcgcag atcgccgacg ggatcgcggc gatcgaggcg gagatcggcc
     5521 gtcggccgcg cggcatgtgg ccatccgagg ggtcggtgtc ggcggccgcg gtcgaggcga
     5581 tggcggcggg cggcctcgtc tgggcggcga gcgacgccga cgtgctgcgc gcctcggagc
     5641 gggacggcga cgccgatccg taccggccct ggcacctcga gggggccgcg gcgccggtcg
     5701 cgctcctgtt ccgcgaccac gatctctccg accgggtcgg cttcgtctac agcaagcgcg
     5761 atccggacga ggcggcctcg gagctcctcg ccgaggcgcg cgcccgcacc agcggcgacg
     5821 agggggcggc gatcctcgtc gcgctcgacg gcgagaaccc ctgggagcag tatccggacg
     5881 ccggtggcgc gttcctgcgc gccctgtacg gcgcgctcga gaaggactcc gaggtcgtgc
     5941 cgctcccggt ggaggaggcg atcgctgcct gtccgcgccg cggcaccatc cgccggctgc
     6001 gcgccgggtc ctggatcgag gcgtcgttcg gcacctggat cggcgggccg gagaagaacc
     6061 gggcctggtc gctcctcggc gagacgcgtc ggcggctgca gccggcgctc gaggacccgg
     6121 accggacccc atcgcaccgg gcggcgtggg tggcgctacg ggcggcggag gggagcgact
     6181 ggttctggtg gctggacggc cagttcgaga acctgtaccg ggccgagttc gacgcgctgt
     6241 tccgcggcca cctgcgccag gcgtgcgagg cggcgggcgt cgccccgccg gaagggatcg
     6301 gctggccgat catcgccggg cgcgatcggg acgtgctgcg ggccccgtcg ctcgatcccg
     6361 ccatcgacgg cttcgaaagc gactacttcg agtggtacgc cgccacgccg gtcgacggcg
     6421 ccacgctcgc cgcgggcggc accatgcagc gggccggccg gccggtcggg gcgctgcgct
     6481 gcgccgtcac gccccgcggc gagctgtcgc tgcggctcga cccgccggcg cccgccgcgg
     6541 cgccgttcgc cgggacgcgg ctcagcctgc ggatcgccgt gtcggagggg ccggtcctcg
     6601 aatccaggct ggcgctcgac gcggacggga acctcgcctc cgccgagccg gacggcgtgc
     6661 gcgcgcgggc gcgcaagatc ctcgaggtgg cggtgccggc gcgggcgatc ctcgcggccg
     6721 gcccgtcgtc ggcccgcctg tcgatcgtcc tcgagcacga ggggatcgcg gtgccgctgc
     6781 gcgacctgga gt
//
LOCUS       DASRIQ010000103         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3691405, whole genome shotgun sequence.
ACCESSION   DASRIQ010000103 DASRIQ010000000
VERSION     DASRIQ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3691405"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..>2146
                     /locus_tag="VFD06_02475"
     CDS             22..>2146
                     /locus_tag="VFD06_02475"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HZN02431.1"
                     /translation="MSAVPSPKAAAPVRLVLALHFHQPVGNFDSVFREAIDRSYDPLV
                     DHFERHPGVRAGFHLSGCLIEWLEAHDRGLLDRVLALVGRGQVEPLGGGFYEPILPVI
                     PRADALDQIERLSAWWEKRAGLRPRGAWIAERVWEPALADLLSEAGVQYTILDDQHLR
                     FAGLLDERFTGIFATERAGRGVAFFPSDSALRYLIPFRPIATVREHLEAARTLAPPDA
                     AAGPVLTYGDDAEKFGLWPETHAWVYGERWLEQFMSLLEEPDGPAIGVQPADVIAGRP
                     PARKVYVPNASYTEMLEWALPAASVAAYGRARDAASERAGREAARAFVRGSLWDMFLA
                     RYPEADQLHKHVLWSSRRARGLEASAPERGPALTAALRAECNCAYWHGLFGGIYYSHL
                     RHGLYHSLLEADALLAKHGSPVVERIDLDGDLEDELVLRAGRMQAFFRPSDAGTLVEL
                     DDLESRFNVTNVLSRFRESYHEGADLTHGGEGEGAVASPHEQKVGIEAKDLEGRAFDL
                     LPLRSLRDFTAAEPPAPEALARFEWMTLLAGRPASWEETARGFRHRIETTDFDADREV
                     TIDAEGALHVRWSVRRGPAGGVFGTLLALTLLTPKDEQRRRYVIAAEAGPGRPRPIEE
                     EPGAPAARPGVSGLILEDRVFGFALDLRVEPAAHLTATPIETLQRAEVRYEKAYQGTL
                     FALCWRLPRAGKASTPQESAASLVLR"
BASE COUNT          270 a          774 c          787 g          315 t
ORIGIN      
        1 ctgggacaag gggggccgtg cgtgagcgcc gtcccgtctc cgaaggccgc ggcgccggtg
       61 cgcctcgtcc tcgcgctcca cttccaccag ccggtcggga acttcgactc cgtcttccgc
      121 gaggcgatcg accgctccta cgacccgctc gtcgatcact tcgagcgcca cccgggcgtg
      181 cgcgccgggt tccacctttc cggctgcctc atcgagtggc tggaggcgca cgatcgaggg
      241 cttctggatc gcgtcctggc gctggtgggg cgtggccagg tcgagccgct cggcggcgga
      301 ttctacgagc cgatccttcc cgtcatcccg cgcgccgacg ccctcgatca gatcgagcgg
      361 ctgtcggcgt ggtgggagaa gcgcgccggg ctccggcccc gcggcgcctg gatcgccgag
      421 cgggtctggg agccggcgct ggcggatctc ctgtcggagg ccggggtgca gtacaccatc
      481 ctcgacgacc agcacctgcg cttcgcgggg ctcctcgacg agcgcttcac cgggatcttc
      541 gccaccgagc gggccggccg gggggtcgcc ttcttcccct cggactcggc gctccggtat
      601 ctcattccgt tccggccgat cgcgacggtg cgcgagcacc tcgaggcggc ccgcacgctc
      661 gctccgccgg acgctgcggc cgggccggtc ctgacctacg gcgacgacgc cgagaagttc
      721 ggcctctggc cggagacgca cgcctgggtc tacggcgagc gctggctcga gcagttcatg
      781 tcgcttctcg aggagccgga cggcccggcg atcggggtgc agccggccga cgtgatcgcc
      841 ggtcgcccgc cggcgcgcaa ggtgtacgtg ccgaatgcct cctacaccga gatgctggaa
      901 tgggcgctgc cggccgcatc ggtcgcggcc tatgggcggg cgcgcgacgc cgcgagcgag
      961 cgggccggcc gcgaggcggc ccgagccttc gtgcgcggca gcctttggga catgttcctg
     1021 gcccgctatc ccgaggccga ccagctgcac aagcacgtgc tctggtcgtc gcgccgcgcc
     1081 cggggcctcg aggcgtcggc gcccgaacgg ggcccggcgc tgaccgcggc gctgcgcgcc
     1141 gagtgcaact gcgcctactg gcacggcctc ttcggcggga tctactacag ccacctgcgt
     1201 cacgggctgt accactccct tctcgaggcg gacgcgctgc tggcgaagca cggatctccg
     1261 gtcgtggagc ggatcgacct cgatggcgat ctcgaggacg agctggtgct gcgggccgga
     1321 cggatgcagg ccttcttccg gccgtcggac gccggcaccc tcgtcgagct cgacgatctc
     1381 gaatcgcgtt tcaacgtcac caacgtcctg tcgcgcttcc gcgagtcgta ccacgagggg
     1441 gccgacctga cccacggcgg cgagggggag ggcgcggtcg cgtcgccgca cgagcagaag
     1501 gtcggcatcg aggcgaagga tctcgagggg cgcgccttcg acctgcttcc gctccgcagc
     1561 ctgcgggact tcacggccgc cgagcccccg gcgcccgagg cgctcgcacg cttcgagtgg
     1621 atgaccctgc tcgccgggag gccggcgtcg tgggaggaga cggcccgcgg cttccgtcac
     1681 cggatcgaga cgacggattt cgatgcggac cgcgaggtga cgatcgacgc cgaaggggcg
     1741 ctgcacgtcc gctggagcgt ccggcggggc cccgccggcg gcgtcttcgg caccctcctc
     1801 gcgctgaccc tcctgacccc gaaggacgag cagcgccggc gctatgtgat tgcggcggag
     1861 gccggaccgg ggcgcccgcg tccgatcgaa gaggaaccgg gggcgccggc ggcgcggccc
     1921 ggcgtgagcg ggctcatcct cgaggatcgg gtcttcggat tcgccctcga cctgcgcgtc
     1981 gagccggcgg cgcacctcac ggcgacgccg atcgagacgc tgcagcgggc agaggtccgc
     2041 tacgagaagg cttatcaggg gaccctcttt gcgctatgct ggcgcctccc gcgcgcggga
     2101 aaggcttcga cgccgcagga gagcgccgcg agcctcgtcc tgcggt
//
LOCUS       DASRIQ010000104         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1093807, whole genome shotgun sequence.
ACCESSION   DASRIQ010000104 DASRIQ010000000
VERSION     DASRIQ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093807"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1993)
                     /locus_tag="VFD06_02480"
     CDS             complement(65..1993)
                     /locus_tag="VFD06_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HZN02432.1"
                     /translation="MRLRPVLWMLLAAVLGPAPAAPLRADPAADLAKGQDAVLLEERL
                     EIAVASATSARVRYTNRTQVLTQRGAERYDVAFVGYGKGVTIRDLRGAVVSPAGKRAE
                     VKRQQIADAAAFESYALYADSMVRAINFPGVVPGSTVEYSWEQEIGNLFFLPDRFDLQ
                     EAIPVRIKVLTVQAPADFLLRAPVRGASPEYEREERDGLVTQRWTVRDVAPLKREPLL
                     PPIEDLVPNVTIVPRRIVWSGVATIDAANWSGIGRFYWDLARDRMVPSPEVAEAARAL
                     VQGIASPDDRLRALFEFVQGKINYVSISLGVGGWQPHTNGDVYRFRYGDCKDKATLLI
                     AMLRAVDLEALPVLIRTRDAGRIDPDNPGVVFNHAIVAVPRPDGYLFLDPTDTVTPFG
                     DLPGDDQGVPVVVVKSDGTGEFLETPLSPPERNRRDRTVRGSISAAGNFEGTLDIAAW
                     GQRRVDMAGFLDSRPSEREDDLEDLIASLCPGAIMKGYEVAPPRGPQDPIRVTIRFTV
                     PQFVTRAAGLEILSPQIVRLPWLAQMATAPDRTHALYLDYPRQENSEVRLALPPGRTL
                     KKVPADRERSAAGLTVTSRYAVKQDGDHQVLEVRRTISISRREVPASEFPALRTLLGS
                     LAEEDASAVTLVSAASGS"
     gene            2052..>3014
                     /locus_tag="VFD06_02485"
     CDS             2052..>3014
                     /locus_tag="VFD06_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HZN02433.1"
                     /translation="MIPFRPTRTRILLLALVAAALVVSARPSAAAKRVAAGAAADWPE
                     ITAAEKALAKVDEDPDAGAVILQQSRDGRIQRKADDYVNVLRYHWRLKVLNDRGKEFG
                     EVHIRAGKYTRVDAIEARTIRPDGSIVPVPPDQIFEKVAFQVGSIRQVEHVFNFPAVE
                     PGAILEYRYVRHDNGLLFIDPYLFGGDAYTLRSHVSQGFLAGTSYAILCTLCPPEPPR
                     WGEWRDGNQKGQVFTRDLTNVPAARGEALMPPPRETTPRLDMSLNVWQGQYSEALGRV
                     DKFFVDWESVARWVGTSYGKTVKSGLSEVAPVAEGWAAGAADAPA"
BASE COUNT          466 a         1031 c         1074 g          443 t
ORIGIN      
        1 cagaatgaag cacctgggcg ccgtcacggt tccgagatcg ggggttacga cagtgtgact
       61 ggagtcatga gccggacgcc gcgctgacga gggtcaccgc ggaggcatcc tcctcggcca
      121 gcgatcccag aagcgtacgc agagcgggga attccgaagc aggcacttcg cggcggctga
      181 tggagatggt gcgacgcacc tcgaggacct ggtgatcccc gtcctgcttg acggcgtacc
      241 gggaggtcac cgtcaggccc gccgccgagc gctcccgatc ggccggcacc ttcttgagcg
      301 tgcggccggg cgggagcgcc aggcggactt cggagttctc ctggcgcggg tagtcgagat
      361 acagggcatg ggtacggtcc ggggcggtcg ccatctgcgc cagccagggc agccggacga
      421 tctgcggcga caggatctcg agtcccgccg cacgggtgac gaactgcggc acggtgaagc
      481 ggatcgtcac ccggatcgga tcctgcggcc cgcggggcgg agcgacttca taccccttca
      541 tgatcgctcc cggacagagg gaggcgatca ggtcctcgag gtcgtcctcc ctttcgctcg
      601 gtcggctgtc gaggaacccc gccatgtcca cccggcgttg tccccaggcg gcgatgtcga
      661 gcgtcccctc gaagttgccg gccgcactga tcgagccccg caccgtccgg tcgcgccgat
      721 tgcgctcggg aggggagagc ggagtttcga ggaactcccc cgtgccgtcc gatttcacga
      781 cgacgaccgg tacgccctga tcatcgccgg ggaggtcgcc gaacggcgtc accgtgtcgg
      841 tcgggtcgag gaacaggtaa ccgtccgggc gcggcaccgc gacgatggcg tggttgaaca
      901 cgacgcccgg attgtccggg tcgatccgcc ccgcgtcgcg cgtccggatg agcaccggca
      961 gcgcctcgag atccaccgca cgcagcatgg cgatgagcag cgtcgccttg tccttgcagt
     1021 cgccgtagcg gaacctgtac acgtcgccgt tcgtgtgcgg ctgccagccg ccgacgccga
     1081 gcgaaatcga cacgtagttg atcttcccct gcacgaactc gaacagggcg cgcagccggt
     1141 cgtcagggga ggcgatcccc tgcacgaggg cgcgggccgc ctcggcgacc tccggcgacg
     1201 ggaccatgcg gtcacgcgcc agatcccagt agaagcggcc gatgccgctc cagttggccg
     1261 cgtcgatggt ggcgacgccg gaccagacga tgcggcgggg aacgatcgtc acgttcggca
     1321 ccaggtcttc gatcggcggc agcagcggtt cgcgcttcag gggcgccacg tcccgcacgg
     1381 tccagcgctg cgtcaccagg ccgtcccgct cctcccgttc gtattccgga ctcgcccccc
     1441 gcacgggggc gcgcagcagg aagtcggccg gagcctgcac cgtcaacacc ttgatgcgga
     1501 ccgggatcgc ctcctgcagg tcgaagcgat cggggaggaa gaagagattg ccgatctcct
     1561 gctcccacga gtactcgacc gtcgatccgg gaaccactcc ggggaagttg atcgcgcgga
     1621 ccatcgagtc ggcgtacaga gcgtacgact cgaaggccgc ggcgtcggcg atctgctgcc
     1681 gtttcacctc ggcccgtttg ccggccgggg agacgaccgc gccgcgcagg tcgcggatcg
     1741 tcaccccctt gccgtagccg acgaacgcga cgtcgtagcg ctcggcgccg cgctgggtca
     1801 gcacctgcgt ccggttggtg tagcgcacgc gcgccgaggt ggcggaggcg acggcgatct
     1861 ccaggcgctc ctcgagcagg accgcatcct gtcccttcgc caggtcggcg gccggatcgg
     1921 cgcggagcgg cgcggcggga gccggtccca ggacggcggc gaggagcatc cagagcacgg
     1981 ggcgcaggcg catgtcgggt agaataccgc caactcgccc ggactcgttc gtcgtcacca
     2041 aggaggcacg tgtgattcca tttcgcccga cccgcacccg catcctgctg ctcgccctcg
     2101 tcgccgccgc gctcgtcgtg tcggcccggc cgtccgccgc cgcgaaacgc gtcgccgcgg
     2161 gtgcggccgc ggactggccg gagatcaccg cggccgagaa ggcgctcgcc aaggtcgacg
     2221 aggacccgga cgccggggcg gtcatcctgc agcagtcgcg cgacgggcgg atccagcgga
     2281 aggcggacga ctatgtcaac gtcctgcgct accactggcg cctcaaggtg ctcaacgatc
     2341 ggggcaagga gttcggcgag gtgcacatcc gcgccggcaa gtacacgcgc gtggacgcga
     2401 tcgaggcgcg caccatccgt cccgacggat cgatcgtccc ggtgccgccg gatcagatct
     2461 tcgaaaaggt ggccttccag gtcggcagca tccggcaggt cgagcacgtc ttcaacttcc
     2521 cggcggtgga gccgggggcg attctcgagt accgctacgt ccgtcacgac aacggactgc
     2581 tgttcatcga cccgtatctc ttcggcgggg acgcctacac gctccgctcg cacgtctcgc
     2641 aggggttcct cgcggggacc agttacgcca tcctgtgcac gctctgcccg cccgagccgc
     2701 cccggtgggg ggagtggagg gacgggaacc agaaggggca ggtgttcacg cgcgacctga
     2761 cgaacgtccc ggccgcgcgc ggcgaggcgc tgatgccgcc gccgcgtgag acgacccccc
     2821 gcctggacat gtccttgaac gtctggcagg ggcagtactc ggaggcactc ggccgggtgg
     2881 acaagttctt cgtcgactgg gagtcggtgg cgcgctgggt cggaacgtcc tacggcaaga
     2941 cggtgaagtc gggtctgtcc gaggtcgccc cggtcgcgga gggatgggcc gcgggggccg
     3001 ccgatgcgcc cgcg
//
LOCUS       DASRIQ010000105         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2910614, whole genome shotgun sequence.
ACCESSION   DASRIQ010000105 DASRIQ010000000
VERSION     DASRIQ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2910614"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1136)
                     /gene="lpxD"
                     /locus_tag="VFD06_02490"
     CDS             complement(48..1136)
                     /gene="lpxD"
                     /locus_tag="VFD06_02490"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZN02434.1"
                     /translation="MPRYTLSEIAARVGGEVRGDPGRPILGVQPLDSAGPDDLSFLAH
                     PRYRGAAAESRAAALLVRRGEDLAGRDLLLVEQPYTALAEIIGLFHPPVAPSGRVSPL
                     AWVDETARLGTGVTVGPFAVLGARAEVGDGAVLMAGVTIGDGAAVGPGSVLHPRVVIY
                     PGCVVGARVVIHAGAVIGSDGFGFGEEAGGRAKIPQVGIVRVEDDVEIGAGTTIDRAT
                     FGATIIGRGSRIDNLVQVGHNVEIGPGSVLVAQSGIAGSTRLGPGAILAGQAGVAGHL
                     RIGAKAIVGAKSAALADVPDGAFVVGHPAVDHREWKRAQAALRRLPLVLRRLQALEAA
                     ALKGRPEVPAASRERSRAKRTRRPRRTR"
     gene            complement(1142..1723)
                     /locus_tag="VFD06_02495"
     CDS             complement(1142..1723)
                     /locus_tag="VFD06_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HZN02435.1"
                     /translation="MSRSILPFVLAFAVAGPTFAATPGEAMKIGVFDPEVLWKQTEVG
                     KRYNQDLSAARDRLQAQIDKKTEDVEALKSRLRQQQQSLSEDKINEMKKDIIGKQTEL
                     ERLNEDATKEMKYQLGEVQGRFQEMLVRTLDAFGKEKGFSLILNRGVVDFSAAGIDIS
                     QDLILKFNEMHKAAATPAGPAKKPEAGKEPPKN"
     gene            complement(1846..>2819)
                     /locus_tag="VFD06_02500"
     CDS             complement(1846..>2819)
                     /locus_tag="VFD06_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZN02436.1"
                     /translation="HGLRSQSSVEYAGGFLGGDNDFVKFRQDATLYLPGFMRRHYVAL
                     NLSAGYVRPINDAAFVPQYERYYLGGERSLRMFRTRSVSPIRTDTDVNHNGFIDVPED
                     RRPDGVFGPCEDLNGNGIQDPNEFDRGNCLLDPSEDANGNFLLDSEDFNRNGILDPGE
                     DLNGNGVLDNEDVNGNGLLDFSEDRPDGVFSPFCAADDRNMNGVRDGAEGDFGNCRLD
                     YGEDTNGDGVFGTVLPGGDKYVQFNVEYAVPVSDTVEFVIFYDAGNAYDNGVNPSFDD
                     LRVDFGLEMRFYLPIFQAPLRLIYGVIQDPIPGEDTSNFIFSIGTTF"
BASE COUNT          388 a          986 c          960 g          485 t
ORIGIN      
        1 ttggatgtcg aggatcgtgg cgctggattc catgctgcgg tcctccttca acgtgtccgg
       61 cggggacggc gcgtgcgctt cgcccggctc cgctcccgcg atgcggccgg cacctccgga
      121 cgtcctttga gggccgccgc ttcgagcgcc tgcaggcggc gcaggaccag cggcagccgg
      181 cgcagcgcgg cctgcgcgcg cttccattct ctgtgatcga ccgcggggtg gcccacgacg
      241 aacgcgccgt cgggaacgtc cgcgagggcc gccgacttcg cccccacgat cgccttcgcc
      301 ccgatgcgca ggtggcccgc gaccccggcc tgtccggcga ggatcgcacc cggtccgagg
      361 cgggtgctcc cggcgatccc cgactgggcc accagcaccg aaccgggccc gatctccacg
      421 ttgtggccga cctgcaccag gttgtcgatc cggctgccgc gcccgatgat ggtggccccg
      481 aaggtggcgc ggtcgatcgt cgtcccggcg ccgatctcga cgtcgtcctc gacgcgcacg
      541 attccgacct ggggaatctt ggcgcgtccc ccggcctcct cgccgaagcc gaacccgtcg
      601 ctgccgatca ccgcccccgc gtggatcacc acgcgcgccc cgacgacgca tcccgggtag
      661 atcaccacgc gggggtggag gacgctcccg gggcccaccg cggcgccgtc tccgatcgtg
      721 accccggcca tcaggacggc accgtcgccg acctccgcac gcgcgccgag gaccgcgaac
      781 ggcccgaccg tcactccggt gccgaggcgc gcggtctcat ccacccacgc gagcgggctg
      841 acccggccgg acggcgccac cggcgggtgg aagaggccga tgatctcggc cagagcggtg
      901 tacggctgtt cgaccagcag caggtcgcgt cctgccaggt cctcgccgcg ccgcacgagc
      961 aatgccgccg cgcggctttc cgccgccgcg ccacggtagc gcgggtgcgc caggaagctg
     1021 aggtcgtcgg ggccggcgct gtcgaggggc tggacgccca gaatcggccg gccggggtcc
     1081 ccgcgcacct cgcccccgac ccgcgccgcg atctcggaga gcgtgtagcg gggcaagagg
     1141 gtcagttctt cggcggctcc ttgcccgctt ccggtttctt cgccggacca gcgggagtcg
     1201 ccgcagcctt gtgcatctcg ttgaacttca agatgagatc ctggctgatg tcgataccgg
     1261 cggcgctgaa gtcgaccacg ccccggttga ggatgaggct gaaccccttc tccttgccga
     1321 aggcatccag cgtcctgacc agcatctcct ggaagcgccc ctggacctca ccgagttggt
     1381 acttcatctc cttcgtcgcg tcctcgttga ggcgctcgag ctcggtctgt ttcccgatga
     1441 tgtccttctt catctcgttg atcttgtcct cgctcaggct ctgctgctgc tgccgcaggc
     1501 gggacttgag cgcctcgacg tcttccgtct tcttgtcgat ctgcgcctgc aggcgatcgc
     1561 gcgccgcgga caggtcctgg ttgtacctct tgccgacctc cgtctgtttc cagaggacct
     1621 cgggatcgaa gaccccgatc ttcatcgcct cgcccggcgt ggccgcgaac gtcgggccgg
     1681 cgacggcgaa cgcgaggacg aagggcagga tggagcgtga catcatgggt ctccttgagg
     1741 gtgcgatttc gagccgccga ctataccaca gcgatgcggg cccgtccggg acgcctgccc
     1801 gccgcgcgga gccgcctcgg ggcctgcccg atgggcaggc gtttcctaga acgtggtgcc
     1861 gatgctgaag atgaagttcg aggtgtcctc gcccgggatc gggtcctgga tgacgccgta
     1921 gatgagccgc agcggtgcct ggaagatcgg caggtagaag cgcatctcca ggccgaagtc
     1981 gacccggagg tcgtcgaagc tgggattgac gccgttgtcg taggcgttgc cggcgtcgta
     2041 gaagatcacg aactcgaccg tgtcgctcac cggcaccgcg tactccacgt tgaactggac
     2101 gtacttgtcc ccgcccggga gcacggtccc gaagacgccg tcgccgttcg tgtcctcgcc
     2161 gtagtccagg cggcagttcc cgaagtcacc ctcggctccg tcccgcaccc cgttcatgtt
     2221 gcggtcgtcg gcggcgcaga acgggctgaa gacgccgtcc ggccgatcct cggagaagtc
     2281 gagcaggccg ttgccgttga cgtcctcgtt atcgaggacg ccgttgccgt tgaggtcctc
     2341 gccggggtcg aggatgccgt tgcggttgaa gtcctcgctg tccaggagga aattgccgtt
     2401 cgcgtcctcg gacggatcga gcaggcagtt gccgcggtcg aactcgttcg ggtcctggat
     2461 cccgttgcca ttgagatcct cgcacggtcc gaagacgccg tccggtcggc ggtcctcggg
     2521 cacgtcgatg aatccgttgt ggttgacgtc cgtgtcggtg cggatcgggc tgacgctccg
     2581 cgtgcggaac atcctcaggg aacgctcgcc gccgaggtag tagcgctcgt actgcggcac
     2641 gaaggcggcg tcgttgatcg gccgcacgta gccggccgac aggttcaggg cgacgtagtg
     2701 ccggcgcatg aagcccggca ggtacagggt cgcgtcctgg cggaacttga cgaagtcgtt
     2761 gtcccccccg aggaagccgc cggcgtactc gacgctcgac tggctgcgga gcccgtgtg
//
LOCUS       DASRIQ010000106         8868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_813109, whole genome shotgun sequence.
ACCESSION   DASRIQ010000106 DASRIQ010000000
VERSION     DASRIQ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813109"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VFD06_02505"
     CDS             <1..1264
                     /locus_tag="VFD06_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HZN02437.1"
                     /translation="SDLAGLLLVADLLRGLPEADEWRETATAGLHAHAAVQTLADGAD
                     FEASIPYHRLTTEMLLAAVLVLERRGGDAAALRPLLRRRIDYTAHYTKPDGLAPQIGD
                     NDDGRFLVLGEHGADRRDHRALLSVAAVLFDDPALLALAGDRCEDALWVHGPASLDRV
                     AARARAIEPRVTSALFREAGTVILRGGGLYVHVEAGGVGLSGHGGHAHNDTLSIEVQA
                     EGEDLIVDPGTGGYTADLVVRDRFRSTAAHNTVRVDGSEINPLPANPFRLPGIDRPSI
                     ARAVFRRHFDLIEALHRGYARLADPVVHRRILLLNRTTRRLLIEDALEGKGTHRLEWF
                     FHLAPRVLGRIEEDGRSFLGHAGKVRFRLRADQAPEGVRFTLAPDLFSPGYGRTEPAT
                     TLCLAWEGGLPVSARFHLCVEDVRREEA"
     gene            1261..3438
                     /locus_tag="VFD06_02510"
     CDS             1261..3438
                     /locus_tag="VFD06_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HZN02438.1"
                     /translation="MKQVLLREGRAVVEEMPDPVASPGRVLVRTAFSLVSAGTERAVL
                     EGTSKASLLSAAGNPGAIGKALDVLRREGAAGLLDRVRARAVPPEAVPGYAASGLVES
                     VGRGIADLPPGTAVACAGSGYACHAERIAVPRRLVVPVPRGVPLDEAAFATLGAIALQ
                     GVRRSGIAVGESAVVLGLGLVGALTAQILKAAGAIVLGFDADRERAARARAVGIEAFD
                     LAARDPRDEVPRLTSGLLADAVLVCAASDGSEVTNLALRLARKKGRVVLVGATGLDVD
                     RALLYEKELDLLMSTSYGPGRYDPEYEEHGHDYPPAYVRWTEQRNLEAFLRLLQDGSV
                     RVRPLIDLVVPLGSAPEAYERIAAAEGPRPLGVLLRYSTGETGTSAAAPSMVPGPAPA
                     AARQEIEPIAKGDLGVLLCGAGSFVRSAHVAAIRRLPDSVLRGLVTGSPASAREASRR
                     LGVERSGTDLREMLARDDAPLVLVGTRHHLHAEQALEALRAGRHVLVEKPLCLEEAEI
                     DPLLETARSRRRLLAVGFNRRYSPLATRMREALDRLSGPALAVYRVNAGALPAGHWVQ
                     DRTVGGGRIVGECCHFVDLLQFLLRAPVAGIEAQALPSNGAGVVQGDSFAATLLFPDG
                     SRAVLAYTGLGDPSFPKERLEIFKGGAALWLDDFRALHVHGGPGGSMTLPRQDKGIDG
                     QWRAVAAALRGDASEVIGLGEIASAMRATFRLDRAVRGERCAS"
     gene            3426..4658
                     /locus_tag="VFD06_02515"
     CDS             3426..4658
                     /locus_tag="VFD06_02515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN02439.1"
                     /translation="MRVLMWEHFAPGGPIRVGGHHFARRFLRRGDRVVWCAGPLAPWN
                     LAGGNDEIRARRRLWKQGGSRLEDGLFAYTPLTWLPHRRRPLLDTAAVARRTLSFTTP
                     RFDRVLGRAGFDDVDLLFMEPGAPMTALLDRYPRARWVYRMCDDTAAFPDTPRSFPEI
                     EREVLERADLVLATARALEQRARASGARRVLYLPNACEPERFAAPPRADAGLASLGRP
                     LAIYVGALDHWFDRDLLAETARLLPGWRFALVGPERGAAGPLASLPNVHRVGPRRYED
                     LPACFAAADAGLVPFRLTPMTHAIHPIKVYEYLAAGLPVVATPMAETAAMQAPLTLAS
                     DAAAFAAALVSACDRSGAPGGDAARSARIAWARRHTWDERFERLAAALADAGVDRRND
                     RVMTPEAGTVRAAAGGRR"
     gene            4655..5866
                     /locus_tag="VFD06_02520"
     CDS             4655..5866
                     /locus_tag="VFD06_02520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN02440.1"
                     /translation="MKRAVALHPEVRRLLAARPAPAAPVASAPRRVLHLLSTLQRGGT
                     EMALLRLLPRMDPAAWTFRVAWLHGPADLGDDFARACGAEPMPLGLRFKADVVVLPRL
                     VALMRRERIDLLQTHMDLADYYGAAAARLAGTRLISIRQNADEFRTRRTWKRPPFLLL
                     EHLSYAAADAVIAVSDGLVEFLARAEGLPRHKTVVIPNGVDETIAARAPESKAARQAL
                     GLPEGPLIGSVGRLAEQKGHLYLIRALPEIRSGVPGARVVLAGEGPERARLEAEAKRL
                     GVGDAVIFLGHRDDVLTVLAALDLFAFPSLWEGLPMALLEAMTLAKPSVAARGVGIDD
                     LVTDDVTGLLVEPRDAAGLAAAARRLLRDKETAERLGEAARRLVLRRHTLGRIAQDTD
                     RLYRRVLRGER"
     gene            5863..7035
                     /locus_tag="VFD06_02525"
     CDS             5863..7035
                     /locus_tag="VFD06_02525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN02441.1"
                     /translation="MTAPGPRVLALTVGWGLGGAERLLLLSAPRLRSLGFDVRVGALK
                     EDGPLRGLLEGAGVPCVAFGARHRGDLGALVRLGRYLRRERIDVLHAHLFYANLAARF
                     VGRLANVPVILAAHHDTDVWMGPHHRLLERLTAPLSHRVLACSEAVRRYAIDRHGIRA
                     ERVVTLRNAIDPEAAPAGSGARARARAEFQASEDDRVAGTLGRLDEPKKGLRVFIAAA
                     ARVAAADPHACFVLIGDGPARAGLERKAAALGLGDRVRFLGERTDALSLLPGLDLLVQ
                     PSLWEGFGLSVLEGMAAGVPVVATRVGGLVEVVRDGVDGVLVPPEDAPRLAEAIAALF
                     ADPELRERFGVAGRTRARAEFHVDRLVVETASLYRALLPGGCPDGTRGAAGVGRAA"
     gene            7032..8045
                     /locus_tag="VFD06_02530"
     CDS             7032..8045
                     /locus_tag="VFD06_02530"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZN02442.1"
                     /translation="MTFLDLVRPGLAFALALALSLYLTPLMRRAAIRFGIVDRPDGAL
                     KTQKEPVAYLGGLAIYLSFLLALTASLRFDSIEVLGLLLAGAIVVILGLVDDFGVLTP
                     KIKLAGQVVAVATLINASIYIKLGFLPSPLAIALSFLWLLAITNAFNIIDVMDGLAAG
                     VAAAASLVLLVIAVANGRTTYAILLAALAGALCGFLRSNFEPARIYMGDAGSLFVGLM
                     LGALAMNNSYTTKNLVASLAPVVILGVPVFDMLFVMYIRRRRGLPIMRGSPDHFALRL
                     RRWRLTTRQTVILSWGATLLLGSVGIGMMAGTAAVATALLGGLVVLALGTGLFLKRID
                     MGL"
     gene            8055..>8868
                     /locus_tag="VFD06_02535"
     CDS             8055..>8868
                     /locus_tag="VFD06_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN02443.1"
                     /translation="MRVPVVILGGGLTGLSAAWHLDGVESLVVEREAEPGGLCRSFTE
                     DGFTFDCTGHMLHLREPRFRDLVERLAPGSFAQHERRALIFSQGTFTPYPFQANLHGL
                     PVDTVKACVAGFVEALLRREKDGDPDAQRATFKQWVEAVFGDGIARHFMLPYNAKLWR
                     VDLEEIECGWVSWSIPRPTLREVLDGAFGATVRGLGYNPTFLYPRRGGIRTLPDAFAR
                     RVPGVRTGRSVEAIDARRRLVRLDGGETIEYERLVSTLPLDRLLQCTDGLPDE"
BASE COUNT         1021 a         3198 c         3315 g         1334 t
ORIGIN      
        1 gtccgacctc gccgggctcc tgctcgtcgc cgacctgctg cgcggcctgc cggaggcgga
       61 cgaatggcgc gagaccgcaa cggcgggcct ccacgcccac gccgcggtcc agaccctcgc
      121 cgacggcgcc gacttcgagg cctcgatccc gtaccaccgc ctgacgaccg agatgctcct
      181 cgccgcggtc ctcgtcctcg agcgacgcgg cggcgacgca gcggcgctgc gcccgctcct
      241 ccggcgccgc atcgactaca ccgcccacta caccaagccg gacggcctgg ccccgcagat
      301 cggcgacaac gatgacgggc gcttcctcgt cctcggagag cacggcgcgg accggcgcga
      361 ccaccgcgcg ctcctctcgg tcgcagcggt gctgttcgac gacccggccc tcctcgccct
      421 cgccggggat cggtgcgagg acgcgctgtg ggtgcacggc ccggcgtcgc tcgatcgcgt
      481 cgccgcgcgc gcccgggcga tcgagccgcg ggtcacgagc gccctgttcc gcgaggccgg
      541 gacggtcatc ctgcgcggtg gcgggctcta cgttcacgtc gaagcggggg gcgtgggatt
      601 gagcgggcac ggaggccacg cccacaacga cacgctgtcg atcgaggtgc aggcggaggg
      661 cgaggacctc atcgtcgacc ccggcaccgg cgggtacacc gcggacctcg tcgtgcggga
      721 ccgattccgg tccaccgcag cgcacaacac ggtgcgggtg gacggctccg agatcaatcc
      781 gcttcccgcg aacccgttcc ggctgcccgg gatcgaccgg ccgtccatcg cgcgcgccgt
      841 gttccgccgg cacttcgacc tgatcgaagc gctgcaccgc ggctacgcgc gcctcgccga
      901 tccggtggtg caccggagga tcctgctgct gaaccggacg acccggcggc ttctgatcga
      961 ggacgccctg gaggggaagg ggacgcaccg cctcgagtgg ttcttccatc tcgcgccgcg
     1021 cgtgctcgga cggatcgagg aggacggtcg gtcgtttctg ggtcacgcgg gcaaggtccg
     1081 cttccggctg cgcgccgacc aggcgccgga gggagtccgc ttcaccctcg cgccggatct
     1141 cttctcgcct ggatacggac gcaccgagcc ggcgacgacc ctctgtctcg cgtgggaagg
     1201 ggggctgccg gtgagcgccc gcttccatct ctgcgttgag gatgtacgaa gggaggaagc
     1261 atgaagcagg tgctcctgcg cgaggggcgg gcggtcgtcg aggagatgcc ggaccccgtg
     1321 gcttcacccg ggagggtgct cgtgcgcacc gccttctccc tcgtcagcgc cgggaccgag
     1381 cgggcggtcc tggagggaac ctcgaaggcg tcgctgctct ccgccgcggg gaaccccggt
     1441 gcgatcggga aagcgctcga cgtcctgcgc cgtgaggggg ccgcggggct tctcgatcgg
     1501 gtgcgggcgc gcgccgtgcc gccggaggcg gtgcccggtt acgccgcctc gggcctcgtc
     1561 gagtccgtcg ggcgcggcat cgccgatctg ccgcccggga cggcggtcgc ctgcgccggc
     1621 tccgggtacg cctgccatgc cgagcgcatc gcggtgccgc ggcgcctcgt cgttccggtg
     1681 ccgcgcggcg tgccgctcga cgaggcggcg ttcgcgacgc tcggggcgat cgccctgcag
     1741 ggggtgcgcc gctccggcat cgccgtcgga gaatcggcgg tcgtgctcgg cctcggactc
     1801 gtgggcgccc tgacggcgca gatcctgaag gcggccgggg cgatcgtcct cggcttcgac
     1861 gccgaccggg agcgcgcggc gcgggcccgc gcagtgggga tcgaggcgtt cgatctcgcc
     1921 gcgcgcgacc cacgcgacga agtgccgcgt ctcacctccg ggctgctcgc cgatgcggtc
     1981 ctcgtctgcg ccgcgtcgga cggcagcgag gtgacgaacc tcgcgctgag gctcgcgcgc
     2041 aagaagggac gcgtcgtcct cgtcggcgcc accggcctcg acgtggaccg ggcgcttctc
     2101 tatgagaagg agctcgatct gctgatgagc acgtcgtacg gaccgggacg ctacgacccg
     2161 gagtacgagg agcacgggca cgattacccg cccgcctacg tccgctggac cgagcagcgc
     2221 aatctcgaag cgtttctccg cctcctgcag gacggatcgg ttcgcgtccg gccgctcatc
     2281 gacctggtgg tgccgctggg ctcggcgccg gaggcgtacg aacggatcgc cgccgcggag
     2341 gggccgcgcc cgctcggcgt gctgctgcgt tacagcaccg gggagaccgg gacctccgcg
     2401 gcggcgcctt ccatggtccc cggtccggcg ccagcggcgg cgagacagga gatcgagccg
     2461 atcgcgaagg gggacctcgg cgtgctcctg tgcggcgcag gatcgttcgt gcgctccgcg
     2521 cacgtggcgg cgatccgccg tctccccgat tccgtcctgc gcggtctcgt gaccggctca
     2581 ccggcgagcg cgcgggaggc gtcgcgccgg ctcggggtcg agcgctccgg caccgatctg
     2641 cgcgagatgc tcgcgcggga cgacgcaccg ctcgtcctcg tcgggacccg gcatcacctg
     2701 cacgccgagc aggcgctcga ggccctgcgg gccggccgac atgtcctggt ggagaagccg
     2761 ctctgcctcg aggaggcgga gatcgatccg ctgctcgaga cggcgcgcag ccgccggcgg
     2821 ctcctggcgg tcgggttcaa ccgccgctac agccccctgg cgacgaggat gcgcgaggcg
     2881 ctcgatcggc tgtccggccc ggcgctcgcc gtctatcgcg tcaacgcggg ggcgctgccg
     2941 gcgggacact gggtgcagga ccgcaccgtc ggcggcgggc ggatcgtggg ggagtgctgc
     3001 cacttcgtgg atctcctgca gttcctcctg cgcgcgccgg tcgcggggat cgaggcgcag
     3061 gcgctgccgt ccaacggggc gggggtggtg cagggggatt cgttcgcggc gacgctcctg
     3121 ttccccgacg gctcgcgcgc cgtgctcgcc tacaccgggc tcggcgatcc ttcttttccg
     3181 aaggagcggc tggagatttt caaaggaggg gcggcgctct ggctcgacga cttccgcgcg
     3241 ctgcacgtcc acggcgggcc ggggggatcg atgaccctgc cgcgccagga caaagggatc
     3301 gacgggcagt ggcgcgccgt tgcggcggcg cttcgcggcg acgcgtcgga ggtcatcggg
     3361 ctcggagaga tcgcatcggc gatgcgggcg acgttccgtc tcgatcgcgc cgtgcgggga
     3421 gagcgatgcg cgtcctgatg tgggagcact tcgcccccgg cggcccgatc cgggtcggag
     3481 gccaccactt tgcgcggcgg ttcctgcggc gcggcgaccg ggtcgtgtgg tgcgccggtc
     3541 cgctggcgcc gtggaacctc gccgggggga acgacgagat ccgcgcccgg cggcgcctct
     3601 ggaagcaggg cggatcgcga ctcgaggatg ggctgttcgc ctacacgcct ctgacctggc
     3661 ttccgcaccg gcgccggccg ctgctcgaca ccgccgccgt cgcccgcagg acgctctcct
     3721 tcacgacgcc ccgtttcgac cgcgtcctcg gacgggccgg cttcgacgac gtggacctgc
     3781 tgttcatgga gccgggggcc ccgatgaccg ccctgctcga ccggtacccg cgcgcccggt
     3841 gggtctaccg catgtgcgac gacaccgccg ccttccccga cacgccgcgt tcgtttccgg
     3901 agatcgagcg ggaggtgctc gagcgcgccg atctcgtcct cgccacggcg cgcgccctcg
     3961 agcaacgggc gcgggcctcg ggcgcacggc gcgtgctcta cctgccgaac gcgtgcgagc
     4021 cggagcggtt cgcggccccg ccccgcgcgg acgcggggct ggcgtcgctc ggccgcccgc
     4081 tggcgatcta cgtcggtgct ctcgatcact ggttcgaccg ggatctcctg gcggagacgg
     4141 cgcgcctcct gccgggatgg cgtttcgccc tcgtcggccc ggagcgcggg gccgcggggc
     4201 cgctcgcgtc gctcccgaac gtgcaccggg tcgggccgcg ccggtacgag gaccttcccg
     4261 cgtgcttcgc cgccgccgac gccgggctcg tgcccttccg gctgaccccg atgacgcacg
     4321 ccatccaccc gatcaaggtg tacgagtacc tggccgccgg gctgccggtc gtggcgaccc
     4381 cgatggccga gacggccgcg atgcaggccc cgctcacgct ggcgtccgac gccgcggcgt
     4441 tcgccgcggc gctcgtctcc gcgtgcgatc ggtccggggc tccgggcggt gacgccgcga
     4501 ggagcgcgcg catcgcctgg gcccgccggc acacctggga cgagcgcttc gagcggctcg
     4561 ccgccgcgct cgccgacgca ggcgtggatc gccgcaacga tcgtgtcatg acgcccgagg
     4621 ccgggacggt gcgggcggcg gccggaggac gacggtgaag cgggcggtgg cgctccatcc
     4681 ggaggtgcgc cggctccttg cggcgcggcc ggcgccggcc gcgccggtgg cgtcggcgcc
     4741 gcggcgcgtc ctgcacctgc tctccaccct tcaaagaggg gggacggaga tggcgcttct
     4801 gcgtctcctc ccgcggatgg acccggccgc gtggacgttc cgggtcgcct ggctccacgg
     4861 tccggccgat ctcggcgacg acttcgcgcg cgcctgcggc gccgagccga tgccgctcgg
     4921 gctgcgcttc aaggcggacg tcgtcgtgct cccgcggctc gtggcgctga tgcggcggga
     4981 gcggatcgac ctcctccaga cgcacatgga cctcgccgac tattacggcg ccgccgcggc
     5041 ccgcctcgcg ggaacccgcc tcatctccat ccggcagaac gccgacgagt tccgcacccg
     5101 gcggacgtgg aagcggccgc cgttcctgct cctggagcac ctgtcgtacg ccgccgccga
     5161 cgcggtcatc gcggtctccg acggtctcgt ggagttcctg gcgcgcgccg aggggctgcc
     5221 gcgtcacaag acggtggtca tcccgaacgg ggtcgacgag acgatcgcgg cgcgcgcccc
     5281 cgagagcaag gcggcgcgcc aggcgctcgg gctgcccgaa gggccgctca tcggctcggt
     5341 cgggcgcctg gcggagcaga aagggcatct ctatctcatc cgtgcgctgc cggagatccg
     5401 gagcggagtc ccgggcgctc gggtggtgct cgccggtgag gggccggagc gtgcccgcct
     5461 cgaggcggaa gcgaagcggc tcggggtcgg cgacgccgtg atcttcctcg gccatcgtga
     5521 cgacgtcctg acggtcctcg ccgcgctcga cctgttcgcc ttcccttccc tgtgggaagg
     5581 gctcccgatg gccctgctcg aggcgatgac gctggcgaag ccgtcggtcg cggcgcgcgg
     5641 cgtgggcatc gacgaccttg tgaccgacga cgtcacgggg ctcctggtcg agccgcgcga
     5701 tgccgccggt ctcgcggccg ccgcgcggcg gctcctccgc gacaaggaga cggcggagcg
     5761 gctcggcgag gcggcgcgcc gcctcgtcct gaggaggcac accctcggcc ggatcgcgca
     5821 ggacacggat cgcctctacc gccgcgtgct gcgaggggaa cgatgaccgc ccccggaccg
     5881 cgcgttctgg cgctcacggt cggatggggg ctgggcggcg cggagcgcct gctgctcctg
     5941 tccgcgccgc gcctcaggag cctcgggttc gatgtgcggg tcggcgccct gaaggaggac
     6001 gggcctttgc gcggccttct ggagggcgca ggggtcccgt gcgtcgcgtt cggcgcgcgc
     6061 caccgcgggg atctcggggc gctcgtccgt ctcggccgct acctgcgccg ggagcggatc
     6121 gacgtcctgc acgcgcacct gttctacgcc aatctcgcgg cgcgcttcgt gggacggctg
     6181 gcgaacgtgc cggtcatcct ggccgcgcat cacgacaccg atgtctggat ggggccgcac
     6241 caccggctcc tggagcggct gaccgcccct ctctcccacc gcgtgctcgc ctgctccgag
     6301 gcggtccggc gctacgcgat cgatcgccac gggattcgcg cggagcgggt cgtgacgctg
     6361 cgcaacgcga ttgatccgga ggcggccccc gcggggtcgg gggcgcgcgc ccgtgcccgc
     6421 gcggaattcc aggcgtcgga ggacgatcgc gtcgccggca cgctcggccg gctggacgag
     6481 ccgaagaaag gtctgcgcgt gtttatcgcg gcggcggcgc gcgtcgctgc cgccgatccc
     6541 cacgcctgct tcgtcctcat cggcgatggg ccagcgcgcg caggcctcga gcgcaaggcc
     6601 gcggcgctcg gcctcgggga tcgggttcgc tttctcggcg agcgcaccga cgccctgtcg
     6661 cttctgcccg ggctcgatct gctggtgcag ccgtccctgt gggaggggtt cggtctgtcg
     6721 gtcctcgaag gaatggcggc gggggtgccg gtcgtcgcga cccgcgtcgg cgggctcgtc
     6781 gaggtggtgc gggacggcgt ggacggggtg ctggttccgc cggaggacgc gccgcgcctg
     6841 gcggaggcga tcgccgccct gttcgccgat cccgagctga gggagcgctt cggggtggcg
     6901 gggcggacgc gggcgcgcgc ggagttccac gtcgatcgcc tggttgtcga gaccgcatcc
     6961 ctttaccggg ccctgctccc cgggggctgt ccggatggca cgcgcggtgc ggccggcgtc
     7021 gggagggcgg cgtgacgttt ctggacctgg tgcgccccgg gctggcgttc gcgctcgccc
     7081 tcgccctgtc gctctacctg accccgctga tgcgccgggc ggcgatccgc ttcggcatcg
     7141 tcgatcggcc ggacggggcg ctcaagacgc agaaggagcc ggtggcgtac ctcggggggc
     7201 tggcgatcta cctgtcgttc ctcctggcgc tgaccgcgag cctgcgcttc gattcgatcg
     7261 aggtgctggg gctcctgctc gcgggggcga tcgtcgtcat cctggggctc gtcgacgact
     7321 tcggcgtgct gacgccgaag atcaagctcg ccggccaggt ggtggcggtc gccaccctca
     7381 tcaacgcctc gatctacatc aagctggggt tccttccttc gcccctggcg atcgcgctgt
     7441 cgttcctgtg gctgctggcg atcaccaacg ccttcaatat catcgacgtc atggacggcc
     7501 tcgccgccgg cgtcgccgcg gccgcgtcgc tggtcctgct cgtcatcgcg gtcgccaacg
     7561 gccgcaccac ctacgccatc ctcctcgcgg ccctcgccgg tgccctgtgc ggcttcctgc
     7621 gctcgaactt cgagccggcg cgcatctaca tgggggacgc cggaagcctc ttcgtcggcc
     7681 tgatgctggg ggcgctggcg atgaacaact cgtacaccac gaagaacctg gtcgcgtcgc
     7741 tggcgccggt ggtcatcctc ggcgtgccgg tcttcgacat gctcttcgtc atgtacatcc
     7801 gccgccggcg cggcctgccg atcatgcgcg ggagcccgga ccacttcgcc ctgcgcctgc
     7861 ggcgctggag gctgacgacg cgccagacgg tgatcctgag ctggggggcg acgctgctcc
     7921 tcgggtctgt cggaatcggg atgatggccg ggaccgccgc ggtcgcgacc gcacttctcg
     7981 gcgggctcgt ggtgctggcg ctcggcaccg gcctgttcct gaaacgcatc gacatggggc
     8041 tctaggggga gatcgtgcgc gttccggtgg tgatcctcgg aggaggtctg accggtctgt
     8101 cggccgcctg gcacctcgac ggcgtcgagt cgctcgtcgt cgagcgggag gcggagccgg
     8161 gagggctctg ccgctcgttc accgaggacg gtttcacctt cgactgtacc gggcacatgc
     8221 tgcacctgcg cgagccgcgc ttccgcgacc tcgtcgagcg gctggcgccc gggagcttcg
     8281 cccagcacga gcggcgcgcg ctgatcttct cgcaggggac cttcacgccg tacccgttcc
     8341 aggcgaacct gcacgggctg ccggtcgaca cggtgaaggc gtgcgtcgcc ggattcgtcg
     8401 aggcgctcct gcgccgcgag aaggacggag atccggatgc gcagcgggcc accttcaagc
     8461 agtgggtcga ggcggtcttc ggcgacggga tcgcgcggca cttcatgctg ccctacaacg
     8521 ccaagctgtg gcgcgtggac ctggaggaga tcgagtgcgg ctgggtctcc tggtcgatcc
     8581 cgcgcccgac gctgcgggag gtgctcgacg gcgcgttcgg cgcgacggtg cgcgggctcg
     8641 ggtacaaccc gaccttcctc tatccgcgcc gtggcgggat ccggacgctg ccggacgcct
     8701 tcgcgcgccg ggtcccgggg gtccgcaccg gccgcagcgt cgaggcgatc gacgcccgcc
     8761 gtcgcctggt ccgcctcgac gggggggaga cgatcgagta cgagcggctc gtctcgacgc
     8821 tgcccctcga ccggctcctc cagtgtaccg acgggctgcc ggacgaga
//
LOCUS       DASRIQ010000107         6307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2381410, whole genome shotgun sequence.
ACCESSION   DASRIQ010000107 DASRIQ010000000
VERSION     DASRIQ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381410"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..684
                     /locus_tag="VFD06_02540"
     CDS             64..684
                     /locus_tag="VFD06_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02444.1"
                     /translation="MTRDFVRIAALPVALAAALVLLLQDTDAPAAAQATAPAGTEMQF
                     TLDERALLDCLKAVLPQTVTVGSGLFSTELTFLDPSDLVLKEGTASCRVRVKGKTLPV
                     DQVLQPIVRLERDAKTGQYYGVISSLPLTLPGMGKIDLKDALPRLEVPQVVDNLHPFG
                     DRPVGLRFSLRRLAIHERRVEIGADLDLSPGTGTARRTPTPGEGRR"
     gene            681..971
                     /locus_tag="VFD06_02545"
     CDS             681..971
                     /locus_tag="VFD06_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZN02445.1"
                     /translation="MIIELVSFRVKTDRIPDFERHNEEWVRLMRRARGYVTHSLMRSL
                     DSPAEFTSEVRWVNRDYRDRFHAIDDAERRALHQNARDLFDGPPDSRLVETI"
     gene            complement(1035..5345)
                     /locus_tag="VFD06_02550"
     CDS             complement(1035..5345)
                     /locus_tag="VFD06_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02446.1"
                     /translation="MTSAIFLVYEALLFALFGVDLGRDFSLLTPGTRLLAGAAALLPA
                     GLLFVRAIRRRVLRPVRRAGALLALATATLAILVASVEAGSRRMLLRWDEGAPARLQA
                     RADLLEADFKGLLDEIIRPFETRLSESSDRRAAFTLLEQSIADTRLPAERYGLALYTA
                     EGEAYAWAGNSTLAPADLLGGSRGLRFLMAGNEEAPRLYAARVDPFTGLTRVAEFLMR
                     SPAIGRPGEAEAAARLEFLPHWGTIEPASVQVLLARSATEDMAALFAHSGDRHWWRVG
                     RQRQLTLSVPLRAQSGEMLLTSHLADRRAAQELSARRAAARRLTALLAAGMLLLAAAG
                     LERRNPWPAPVRLLLLSLTLWGGRALLLSIGPDDALPPLPVYDVAVYSSSIAWGLFRS
                     PLDLLLTAAAAATQAWLFRAALRHRAPRRVFYRMLCGLGAVAIAVASGIGLHDFLDQL
                     VVQSRVDITRIDFDDGLPARLALQTGLFLLVVSAAALVQALARAALGSPAVPGQRTPD
                     DERRVMPAPLRQALVVLALTVVYVPLLTHAYGRLRRAFFEHDLLPRVMHQEEGRRAVL
                     RDALAVVEQPEFAAAAALDEDPSTSGSLAYRLWSRTPLATMGVASSLRIFDTEGRPLS
                     RFAVNLGVMFENQFWEAKEASGRDPIASPPREGVTIKKSVLFGSRWVPVAHQPARLVV
                     LTVLDDFDNVPLLGAETAYLPLLRARAVSRTNPELMRFEPMTAVFGPSLERLYESGGE
                     IPVPSPGTLAAVERNQRAWVNDEVGEGPASILYAKGPDGVYALAFARRDWVGLVAVCL
                     RLAMLNGLLVLVAAGVMRAVLVVAGRAGGRPALGTTFYARLTTVLLLTALAPMLALGW
                     FVTRVSTRESERDLVAAGLTSLQTVRRVAEDYLNLPDPGYGHDLDDEVVFWLSRVVRQ
                     DINIYRGADLVASSTPELYGSSLLNTRLDGTVYRALELDREPVRLARARVGDLEYLTL
                     SAPMRIDPEGGKGVVSIPLAAQSRAVARKADELADAVLILTSVTVALLAFVGWQVARR
                     VSGPIAALSSAARRVAAGDLQVRVRVSGHDEIGALVEAFNTMAASLETQRQDLSRRRE
                     YIETILTRATAGVLSLDARGVVITANPAAQGLLTPQGNPPGLGDHLPDHLALHPTLAP
                     LARALERSLAGDLEGEVGLELGGADERRLRAVFLPFTPEQGALPGRIVILEDVTEIVR
                     SGRLAAWAEMARRIAHEVKNPLTPIQLSVEHVRRLWHARDARFGVVLIECLDNIQSQV
                     RKLRQMATEFSAYARLPQLRAEPTEVPALLTEALRPYLAAPPAGIDIDSAVPAGLPEV
                     VVDRGVMNRVLVNLIENALQAMPEGGRLEIAAGREQGNGRSGVSITVRDTGTGMPPGV
                     LARIFEPYFSTKSGGTGLGLAIARRAVEEHGGGIDIRSRPGAGTLVTIFLPGRDPQAP
                     "
     gene            complement(5350..5772)
                     /locus_tag="VFD06_02555"
     CDS             complement(5350..5772)
                     /locus_tag="VFD06_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN02447.1"
                     /translation="MVVLAILWLLAAPAATLDPDLVASERQVADAFHRADADRLAASL
                     SQRLKTYVACAALGVGDGYYGSDQMRILLRRLFRDRETVRFRALPPPMAPRSDGTAVA
                     MVAWTYRDAGSPPAEVRLALSFAREADGWRLREIRDLK"
     gene            5874..6161
                     /locus_tag="VFD06_02560"
     CDS             5874..6161
                     /locus_tag="VFD06_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZN02448.1"
                     /translation="MSGTDLCRAAATLDAERVDRVIDTTGLYCPVPIVRTGEAIRSLR
                     VGDRLEILSDDRVILIDLPAWCRAHGHLYEGAREEDGILKLRVRKAERRAR"
     gene            6158..>6307
                     /locus_tag="VFD06_02565"
     CDS             6158..>6307
                     /locus_tag="VFD06_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02449.1"
                     /translation="MTTIPGRRVRAIGLISGGLDSTLAARVLLEQGIDVVGLHFSTGF
                     CMNDHR"
BASE COUNT          953 a         2233 c         2337 g          784 t
ORIGIN      
        1 tcctcgcctt cacacacgcc gcggaaagct gcgaggccgg atgctatact cgccgctcct
       61 gccatgacgc gcgacttcgt ccgtattgcc gcccttcccg tcgccctcgc ggcggcgctc
      121 gtcctgctgc tgcaggacac cgatgccccg gcggcggcgc aggcgacggc gccagccggg
      181 accgagatgc agttcacgct cgacgagcgg gcgctcctgg actgtctcaa ggcggtgtta
      241 cctcagacgg tgactgtcgg aagcggcctg ttcagcaccg agctgacctt cctcgacccc
      301 tccgatctgg tcctcaagga ggggaccgcg tcgtgccgcg tgcgggtcaa ggggaagacc
      361 ctgccggtgg accaggtgct ccagccgatc gtgcgcctcg aacgagacgc gaagaccggg
      421 cagtactacg gagtgatctc gagcctaccg ctgacgctcc cggggatggg caagatcgac
      481 ctcaaggacg cgctgccgcg cctggaggtc ccgcaggtgg tcgataatct gcatcctttc
      541 ggcgatcgtc cggtcgggct gcgcttcagc ctccggcgcc tcgccatcca cgagcgtcgc
      601 gtcgagatcg gcgccgacct cgatctgtcc ccggggaccg gcacggcgcg ccgcaccccg
      661 actcccggcg agggccgacg atgatcatcg agctggtgtc gttccgagtg aagaccgacc
      721 ggatcccgga cttcgagcgt cacaacgagg agtgggtgcg gctgatgcgg cgtgcgcgcg
      781 gctacgtcac ccactcgctg atgcgcagcc tcgacagccc ggcggagttc acctccgagg
      841 tgcgctgggt gaaccgcgac taccgcgacc gtttccacgc catcgacgac gccgagcgcc
      901 gggcgctgca ccagaatgcc cgcgacctgt tcgacggccc gcccgacagc cgcctcgtcg
      961 aaaccatcta gggtccggac tccaccagac gcctcattca ccgccgcgat cccgatgagg
     1021 tagtttttta ggttctaggg ggcttgcggg tcgcggccgg gcaggaagat ggtgaccagg
     1081 gtcccggcgc cgggtcggct gcggatgtcg atgccgccgc cgtgctcctc caccgcgcgg
     1141 cgggcgatcg cgagccccag accggtcccg ccgctcttgg tggagaaata gggctcgaag
     1201 atgcgcgcca gcacccccgg cggcatgccg gtgccggtgt cgcgcacggt gatcgacacg
     1261 ccgctgcggc cgttcccctg ctcgcgcccg gcggcgatct cgagccgccc gccctccggc
     1321 atcgcctgca gggcgttctc gatcaggttc accagcaccc ggttcatgac gccgcgatcc
     1381 accaccactt ccgggagccc cgcgggcacg gccgagtcga tgtcgatccc agcggggggc
     1441 gcggcgaggt agggacggag cgcctcggtc aaaagggccg gcacctcggt cggctcggcg
     1501 cgcagctgcg gaaggcgggc gtaggcggag aattcggtcg ccatctgccg cagcttccgc
     1561 acctgactct ggatgttgtc gaggcactcg atgagcacga cgccgaagcg ggcgtcgcgc
     1621 gcgtgccaga gccggcgcac gtgctccacc gacagctgga tcggcgtcag cgggttcttg
     1681 acctcgtggg cgatgcgccg cgccatctcg gcccaggcgg cgagccggcc ggagcggacg
     1741 atctccgtga cgtcctccag gatgacgatg cgtcccggca atgccccctg ttccggggtg
     1801 aacggaagga agaccgcgcg cagccggcgc tcgtcggcac cgccgagctc gagcccgacc
     1861 tccccttcga ggtccccggc gagcgagcgc tccagcgccc gcgccagcgg cgcgagggtc
     1921 gggtggaggg ccaggtgatc cggaaggtga tccccgaggc cgggcgggtt cccctgcggc
     1981 gtcaggagtc cctgcgccgc cgggttcgcg gtgatgacga cgccgcgggc gtcgagggag
     2041 aggactccgg cggtggcgcg cgtcaggatc gtctcgatgt actcgcggcg acggctcagg
     2101 tcctggcgtt gcgtctcgag ggaggcggcc atggtgttga acgcctcgac gagggcgccg
     2161 atctcgtcat ggccgctcac gcgcacgcgc acctggaggt cgccggccgc cacgcgacgg
     2221 gccgcgctcg acagggcggc gatcggcccg gagacgcgcc gcgccacctg ccatcccacg
     2281 aaggcgagca gcgccaccgt cacgcttgtc aggatgagga cggcgtcggc cagctcgtcc
     2341 gctttgcgcg ccacggcgcg gctctgggcc gcgagcggga tggagaccac cccctttccc
     2401 ccctccggat cgatccgcat cggcgccgac agggtcaggt actcgaggtc gccgacgcgg
     2461 gcgcgcgcca ggcgcaccgg ctcccggtcg agctcgaggg cgcggtacac ggtgccgtcg
     2521 agccgggtgt tgaggaggga cgagccgtac agctccggtg tgctgctggc gacgaggtcc
     2581 gcgccgcggt agatgttgat gtcctggcgg acgacgcggc tcagccagaa gaccacctcg
     2641 tcatcgaggt cgtggccgta gccgggatcg ggaaggttga gatagtcctc ggcgacgcgg
     2701 cgcaccgtct gcagcgaggt cagccccgcg gccaccaggt cccgctccga ttcccgcgtg
     2761 ctgacgcggg tgacgaacca gccgagggcg agcatcggcg cgagcgcggt cagcagcagc
     2821 accgtcgtca gacgcgcgta gaaggtcgtg ccgagggcgg ggcggccgcc cgcccggccc
     2881 gcgaccacga ggacggcccg catcaccccc gccgcgacga gcacgaggag cccgttgagc
     2941 atcgccaggc gcaggcagac ggcgacgaga ccgacccagt cgcgccgcgc gaacgccagg
     3001 gcgtaaacgc cgtcggggcc tttcgcgtag aggatgctcg ccggcccctc gccgacctcg
     3061 tcgttgaccc aggcccgctg gttccgttcc acggccgcca aggtgcccgg agaggggacc
     3121 gggatctctc cacccgactc gtacaggcgc tcgagagacg ggccgaacac cgccgtcatc
     3181 ggctcgaagc gcatcagctc ggggttggtg cgggagacgg cgcgtgcgcg caggagcggc
     3241 agataggcgg tctcggcgcc gagcagcggc acgttgtcga agtcgtcgag gacggtgagc
     3301 accaccaggc gagccggctg gtgcgccacc gggacccagc gcgacccgaa caggacgctc
     3361 ttcttgatcg tcaccccctc gcgcggcggc gaggcgatcg ggtcgcgtcc ggacgcctcc
     3421 ttcgcctccc agaactggtt ctcgaacatg acgccgaggt tgacggcgaa gcggctcagg
     3481 ggacgcccct cggtgtcgaa gatccgcagc gacgaggcga cccccatggt cgcgagcggc
     3541 gtgcggctcc agagacggta ggcgaggctg ccggaggtgg agggatcctc gtcgagcgcg
     3601 gcggcggcgg cgaactccgg ctgctcgacg accgccagcg cgtcgcgcag gacggcgcgc
     3661 cgcccctcct cctggtgcat gacgcgcggc aggaggtcgt gctcgaagaa ggcgcggcgc
     3721 agacgcccgt aggcgtgcgt caacagtggg acgtagacca cggtgagcgc gaggacgacg
     3781 agggcctggc gcagcggcgc cggcatcacc cggcgctcgt cgtccggcgt gcgctgaccc
     3841 gggaccgcgg gggagccgag cgcggcgcgg gcgagcgcct gcacgagcgc ggccgccgag
     3901 accacgagga ggaagaggcc ggtctggagg gccaggcgcg ccggcagacc gtcatcgaaa
     3961 tcgatgcgcg tgatgtcgac gcgcgactgc accaccagct ggtcgaggaa atcgtgcaga
     4021 ccgattcccg acgccaccgc gatcgccacc gctcccaggc cgcagagcat ccggtagaac
     4081 acgcgacgcg gcgcgcggtg tcgcagggcg gcccggaaga gccaggcctg cgtcgccgcg
     4141 gcggcggcgg tcagcaggag atcgagcggc gagcggaaga ggccccaggc gatcgacgag
     4201 gagtagacgg cgacgtcgta gaccggcagc ggcgggagcg cgtcgtccgg gccgatgctg
     4261 agcaggaggg cccgccctcc ccacagcgtc agcgacagga ggagcagccg gaccggcgcg
     4321 ggccaggggt tgcgacgctc gaggccggcg gccgccagga gcagcatccc ggcggcgagg
     4381 agcgccgtca gccggcgcgc cgcggcgcgc cgggcggaca gctcctgcgc cgcgcgccgg
     4441 tcggcgaggt gcgaggtgag gagcatctcg ccgctctggg cgcgcagcgg cacgctcaag
     4501 gtcagctgcc tctgccggcc caccctccac cagtgccggt cccccgagtg cgcgaacagg
     4561 gccgccatgt cctcggtggc ggagcgggcg agcagcacct ggacggaggc cggctcgatc
     4621 gtcccccagt gcggcaggaa ctcgaggcgg gccgccgcct ccgcctcgcc ggggcggccg
     4681 atggccgggg accgcatgag gaactccgcc acccgcgtca ggccggtgaa gggatcgacg
     4741 cgggcggcgt acagacgggg cgcctcctcg ttgccggcca tgaggaagcg caggccgcgc
     4801 gatcctccca ggagatcggc gggggcgagg gtgctgttgc cggcccaggc gtacgcctcc
     4861 ccctcggcgg tgtagagcgc caggccgtag cgctccgccg gcaggcgcgt gtcggcgatg
     4921 gactgctcca ggagcgtgaa ggcggcgcgg cggtcggacg actcggagag gcgggtctcg
     4981 aacgggcgga tgatctcgtc gagcagcccc ttgaagtcgg cctcgagcag atcggcgcgc
     5041 gcctggagcc tcgccggcgc cccctcgtcc cagcgcagga gcatgcggcg cgagccggcc
     5101 tcgacggagg ccacgaggat ggcgagcgtc gcggtcgcga gcgccaggag ggccccggcg
     5161 cggcgcaccg ggcgcagcac gcggcgccgg atcgcgcgca cgaacagcag ccccgccggc
     5221 agcagggcgg ccgcccccgc gagcagccgg gtcccggggg tgagcagact gaagtcgcgc
     5281 cccagatcga ccccgaagag cgcgaacagg agcgcttcgt aaacgaggaa gatcgcgctc
     5341 gtcacggcct catttcagat cgcggatctc ccgcagcctc cagccgtcgg cctcgcgcgc
     5401 gaacgagagc gcgaggcgca cctccgccgg cggcgatccg gcgtcccggt aggtccaggc
     5461 gaccatcgcc accgccgtcc cgtcggagcg cggcgccatc gggggcggca gggcccggaa
     5521 gcggaccgtc tcccgatcgc ggaacagccg gcgcaggagg atgcgcatct ggtcggaccc
     5581 atagtaaccg tcccccacgc cgagcgccgc gcaggcgacg taggtcttca gccgctgcga
     5641 cagggaggcg gcgaggcggt cggcgtcggc acggtgaaac gcgtccgcca cctgacgttc
     5701 cgaagccacc aggtcggggt cgagggtggc ggcgggagcg gccaggagcc acaggatcgc
     5761 gaggacaacc acacggcgag gctagtgcat cgccgccggg aaggtcaaca gaaccgcatc
     5821 ctctgttaga atccctgcgg cttcgcgggt ctgccccgcg cggcaccccc atcatgagcg
     5881 gaaccgacct ctgccgggca gcggcgaccc tggacgccga gcgcgtcgat cgtgtcatcg
     5941 acaccacggg actctactgc ccggtgccga tcgtccggac cggcgaggcg atccgctccc
     6001 tgcgcgtcgg ggaccggctg gagatcctct cggacgaccg ggtgatcctc atcgacctgc
     6061 cggcctggtg ccgcgcccac ggacaccttt acgagggggc gcgtgaagag gacggtatcc
     6121 tgaagctccg ggtgcgcaag gcggagcgga gggcgcgatg accacgatcc ccggccgccg
     6181 cgtccgcgcc atcgggctca tctccggcgg gctcgacagc accctggccg cgcgcgtcct
     6241 gctggagcag gggatcgacg tggtggggct gcacttctcc acaggcttct gcatgaacga
     6301 ccaccgc
//
LOCUS       DASRIQ010000108         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3435641, whole genome shotgun sequence.
ACCESSION   DASRIQ010000108 DASRIQ010000000
VERSION     DASRIQ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3435641"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFD06_02570"
     CDS             complement(<1..319)
                     /locus_tag="VFD06_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN02450.1"
                     /translation="MRVALFDIDGTLVSMGGAARDAFVRALAETVGREVLSDGVSFGG
                     KTDPQIARELLARNGVGADRMEEAIAETVRRYLVHFERDLPKAREARLLPGVRELLEA
                     LSLR"
     gene            455..676
                     /locus_tag="VFD06_02575"
     CDS             455..676
                     /locus_tag="VFD06_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02451.1"
                     /translation="MAKAGLLKGKVDVLKKKIAEKGSSLPPERQRILRKRLKRLQRAR
                     RTATALEARANKKPAAGAADAAAPSAPQA"
     gene            697..1143
                     /locus_tag="VFD06_02580"
     CDS             697..1143
                     /locus_tag="VFD06_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02452.1"
                     /translation="MHLSRTRSAVLSTALGLAALTGCRMDLDEMVMNPNSRDEIINRL
                     LEESVSKQTIMNRLTSDEASKLELAETLLGDNKIKPLAVEKLTADTAQRPKIIDTLLA
                     DPVARQTIIDKLLGDETVRPILQEGLKKPLPPKKETPAEAPAATGT"
     gene            1317..3044
                     /locus_tag="VFD06_02585"
     CDS             1317..3044
                     /locus_tag="VFD06_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02453.1"
                     /translation="MDPLHRDRPQPDDAAPPDLPAGFLPALADAAARLERETDLHAAA
                     ALFLSGLRDHFASRKAVLAVADAQGHDHQWFFTGLSIADISWFHTHQLNEAERQALLT
                     PERRDGRTFSVPVPQCPRQAGLRLNGSQADLLFLPLSGHGGAQLGTLVVDDGRPRSAR
                     SPAIVAALELWTQPLARLLERKRLDHTARNTEMRLRQAQEQLMQADKLSSIGQLISGV
                     AHELNNPLSGVLGFTQLLQASETSPKAKRGLDRIYGEAVRCQKIVQNLLGFARRYKPE
                     KIAHDLVEVVEGVLDLRAYQLAVDNVEIERRYEKNLPPTLLDAHQIQQVVLNLVNNAH
                     QAMMTVTDRPRRLSVALARREDRLHVAIGDTGPGIPPDRLARLFEPFFTTKEPGKGTG
                     LGLSVSLGIVKDHMGNMSVDSAIGRGSTFWFDLPIVAVAASDGTAAAAATTSAKPPHA
                     AGLRVLIVDDEPVLRELLMDLLKSAGHEVESARDGRAALRLALEGRFDVILSDLKMPG
                     LDGQGLYESVCRERPEMARRFVFTTGDVVNPDAEGFLQDSGCAYLSKPFKLETILAVV
                     DQSARRKAA"
     gene            complement(3051..3413)
                     /locus_tag="VFD06_02590"
     CDS             complement(3051..3413)
                     /locus_tag="VFD06_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZN02454.1"
                     /translation="MQAVPHASLFYLPGGVIAAILLGMALTPLREMTNAGNFIFAFVI
                     LIIVVGELGGRGAAVATAACSALSLDFFLTKPYLSLRIEQKHDLIAFAGLALCGLVAA
                     ALGSRHGRRAGDDAPAGR"
BASE COUNT          590 a         1341 c         1248 g          482 t
ORIGIN      
        1 cccggagcga cagcgcctcg agcagctcgc gcacgccggg gagcaggcgc gcctcccggg
       61 ctttcggcag gtcgcgctcg aagtggacca ggtaccggcg caccgtctcg gcgatcgcct
      121 cctccatccg gtcggcgccg acgccgttgc gggccaggag ttcccgggcg atctgcgggt
      181 cggtcttccc tccgaacgag accccgtcgg agaggacctc ccgccccacc gtctcggcca
      241 gggcgcgcac gaacgcgtcc cgggccgccc cccccatgga gacgagcgtt ccgtcgatgt
      301 cgaaaagtgc cacgcgcacg agggcgagtc tagcccagca tcggggcgcc gggcgcccgc
      361 gcgctgcggc gcccctccag cccccctacc ttgacgcacg gcgccccggg tcgctaggat
      421 tccggcttcc ctgacagcgg gacggaggtt gcggatggcc aaggcaggtc tgttgaaggg
      481 caaggtggat gtgctcaaga agaagatcgc ggagaagggg tcctcccttc ctccggagcg
      541 ccagcgcatc ctgcgcaagc gcctgaagcg gctgcagcgg gcgcggcgca cggcgaccgc
      601 gctcgaggcg cgcgccaaca agaagccggc cgcgggcgcg gccgacgcgg cggcaccctc
      661 cgccccgcag gcctgacgtc ggcaagggag accacgatgc acctgtcgcg cacccgttcc
      721 gccgtccttt cgaccgccct gggcctcgcc gccctcaccg gctgccgcat ggatctcgac
      781 gagatggtga tgaaccccaa ctcgcgcgac gagatcatca accgcctgct cgaggagtcg
      841 gtctcgaagc agacgatcat gaaccgcctg acgagcgacg aggcgtcgaa gctcgagctg
      901 gccgagacgc tcctcggcga caacaagatc aagccgctgg cggtcgagaa gctgacggcg
      961 gacaccgcgc agcggccgaa gatcatcgac accctcctcg cggacccggt cgcgcgccag
     1021 acgatcatcg acaagctcct cggcgacgag acggtccggc cgatcctgca ggaagggctc
     1081 aagaagccgc tgccgccgaa gaaagagact ccggcagagg ctccggccgc cacaggcacc
     1141 tagttttccg cggcccgccg ccccggcccc cgatcacgat tccgtcatcg cgtccgcgcc
     1201 gggcgccggt cccgcccgcg cgcgttgaat ccgtcccgcc ccacctctat atttgcgacg
     1261 ccgtcgaggc ggccgagctg tgcgtcaagg tggcgcgcaa cgcatgcaca ggagccatgg
     1321 accccctcca tcgcgaccgc ccacagcccg acgacgccgc tccgccggat ctcccggccg
     1381 gattccttcc ggccctcgcc gatgccgcgg cccgtctgga gcgggagacc gacctgcacg
     1441 ccgccgccgc cctgttcctc tcggggctgc gcgaccactt cgcctcccgc aaggcggtgc
     1501 tcgcggtggc cgacgcgcag gggcacgacc accagtggtt cttcacgggg ctctcgatcg
     1561 ccgacatctc ctggttccac acccaccagc tgaacgaagc ggagcggcag gccctcctga
     1621 ccccggagcg tcgcgacggc cggaccttct ccgtgccggt gccgcagtgc ccgcgccagg
     1681 cggggctgcg gctcaacgga tcgcaggccg acctcctgtt ccttccgctc tccggacacg
     1741 gcggggccca gctcgggacg ctcgtcgtgg acgacgggag gccgcgcagc gcgcgatcgc
     1801 cggcgatcgt ggcggcgctc gagttgtgga cgcagccgct ggcgcgcctc ctcgaacgca
     1861 agcggctcga ccacacggcc cgcaacaccg agatgcggct gcgccaggcg caggagcagc
     1921 tcatgcaggc cgacaagctg tcgtcgatcg ggcagctgat ctccggggtc gcgcacgagc
     1981 tgaacaaccc gctgtccggc gtgctcggct tcacgcagct cctgcaggcg tccgagacga
     2041 gcccgaaggc gaagcgcggc ctcgatcgca tctacggcga ggcggtgcgc tgccagaaga
     2101 tcgtccagaa cctgctcggc ttcgcgcgcc gctacaagcc ggagaagatc gcgcacgacc
     2161 tcgtcgaggt ggtggagggg gtgctcgacc tgcgcgccta ccagctcgcg gtggacaacg
     2221 tcgagatcga gcgccgctac gaaaagaacc tgccgccgac cctgctcgac gcgcaccaga
     2281 tccagcaggt ggtcctcaac ctggtcaaca acgcccacca ggcgatgatg acggtcaccg
     2341 atcggccgcg ccgcctcagc gtggcgctgg cccggcgcga ggaccggttg cacgtcgcca
     2401 tcggcgacac cggccccggg atcccgccgg atcgcctggc ccgcctgttc gagccgttct
     2461 tcacgacgaa ggagccgggg aaggggaccg gcctgggcct ttcggtgtcg ctcggcatcg
     2521 tcaaggacca catggggaac atgagcgtgg acagcgcgat cggccgcggc tcgaccttct
     2581 ggttcgacct gccgatcgtc gcagtcgccg cgtcggacgg gaccgcggcc gccgccgcga
     2641 cgacatccgc gaagccgccg cacgccgccg ggctccgggt cctcatcgtg gacgacgaac
     2701 cggtgctgcg cgagctgctc atggacctgc tcaagagcgc cggacacgag gtcgagagcg
     2761 cccgcgacgg ccgcgccgcc ctgcggctgg cgctcgaggg gcgcttcgac gtcatcctga
     2821 gcgatctcaa gatgccgggg ctcgacggcc agggtctgta cgagtcggtc tgccgcgagc
     2881 ggccggagat ggcccggcgc ttcgtcttca cgaccggcga cgtcgtcaac cccgacgccg
     2941 aggggttcct gcaggattcc ggctgcgcct acctcagcaa gccgttcaag ctcgagacga
     3001 tcctcgcggt cgtcgaccag tccgcccgcc gcaaggcggc ctgaatcgga tcaccgtccg
     3061 gcgggagcgt cgtcgccggc gcgtcgtccg tggcgcgagc cgagcgcggc ggccacgaga
     3121 ccgcagagcg cgaggcccgc gaacgcgatc aggtcgtgct tctgctcgat cctcagggag
     3181 aggtacggct tcgtcaggaa gaagtcgagg ctcagcgccg agcaggcggc ggtcgcgacc
     3241 gcggcgcccc gtccccccag ctcgccgacg acgatgatca ggatgacgaa cgcgaagatg
     3301 aagttcccgg cgttcgtcat ctccctcaag ggagtgagcg ccatgccgag gaggatcgcg
     3361 gcgatcaccc ctccgggaag atagaagagg ctcgcgtgcg ggaccgcctg cattcgtcgc
     3421 atgccctgaa aggtaccgcg caccgcatca agaaagcgtc aagagacggt ccctcacccc
     3481 gttcttgacg tcgccttaac gcggaacggc gtacagatgg cggcatggag gacgcggaca
     3541 ccatgcgggg tccggcgggg gcgctgctgc tggccggcgc aatcatcggc ggcggtctct
     3601 tcgcggagga atctccgacg ccggaaacga gcgctccggc ggaggagccg cggcatatcg
     3661 a
//
LOCUS       DASRIQ010000109         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1601768, whole genome shotgun sequence.
ACCESSION   DASRIQ010000109 DASRIQ010000000
VERSION     DASRIQ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601768"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VFD06_02595"
     CDS             complement(<1..999)
                     /locus_tag="VFD06_02595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN02455.1"
                     /translation="MPDRRALLILFSALFLVMLGVGIVIPNIAYRAAELQATSIEIAL
                     LFTLYSLMQFLFAPIWGRLSDRIGRRPVLIAGLLGGGAGLLLFGAGDRLMTLYLARGL
                     SGLMSSAALPTAMAYVADVTDEKRRGHGMGAMGAAMGLGFIFGPGIGGALARFGHATP
                     FLVAGLLMIVTGLAAAMLLPESLQRPTPASDGAWFHLPAPWKALGSPLLRFYGVAFTV
                     PFAMAALETTFPLLLRDRLGMGAHEMGWMFLFMGTAVFLVQGFALGRVINAAGEERVM
                     RAGLLINAVGFLLVPQATGAIGMTAALVLCGVGNQVMRPTNASLITKRNLGGQGEAI"
     gene            1188..2306
                     /gene="thiO"
                     /locus_tag="VFD06_02600"
     CDS             1188..2306
                     /gene="thiO"
                     /locus_tag="VFD06_02600"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HZN02456.1"
                     /translation="MERAVDVLIVGGGVIGLAIAREAARSGMSVRLLERGETGGEATG
                     ASAGMLAAQLEAHEPGPLLSLSLESRTLYPAFLRALESETGESVDLRREGALVIALDE
                     AAREAIARSASAQRRMGLDVKTLDAVEVRALEPLVSGDTRGGMHLPHEHTVDPRRLAE
                     ALRRAAEAAGAEICTGAEATAILMERKTARGVRLADGRDQRAREVVVAAGAWAGALQA
                     EGIAVPPSEPVRGQMLCFAAPGIVRHVVASEEVYLVPRSDGRLLAGSTMERAGYDRSV
                     TASGMAGIAAAAIRLVPRLGHAAFHSAWAGLRPGAPDGLPVLGRAAPGLLYACGHLRN
                     GIVLTPITAAIVGRLLRGEAAGFDLTPYDPLRFGPVQG"
     gene            complement(2310..>3052)
                     /gene="speY"
                     /locus_tag="VFD06_02605"
     CDS             complement(2310..>3052)
                     /gene="speY"
                     /locus_tag="VFD06_02605"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HZN02457.1"
                     /translation="YDILFDYNVLLDTDNYLRTIMAEKPFNKEMGTAELHYLLGKYLA
                     AREKELGVEDTCVLTAAYRAGVPCYVSSPGDSSIGMNVAELAMRGCGPRIDVTRDVNE
                     TAALVYAAKNGDGKSGLLMLGGGSPKNFVLQTEPQIQEILGLTDRGHDYYIQITDARP
                     DTGGLSGATPSEAVSWGKVDPDKLTDSVVVYTDSTIAAPLMTAYALARVKPRKAKRLY
                     DVREKLVEKLKVDFLAEHERRQKAARKK"
BASE COUNT          442 a         1024 c         1149 g          437 t
ORIGIN      
        1 gatcgcctcc ccctgtccgc cgaggttgcg cttggtgatg agcgacgcgt tggtcgggcg
       61 catcacctgg ttgcccacgc cgcagagcac gagcgccgcg gtcattccga tggcgccggt
      121 cgcctgcggc acgagcagga agcccacggc attgatgagc aggccggcgc gcatcacccg
      181 ttcctccccc gccgcgttga tcacacggcc cagcgcgaac ccctgcacga ggaagacggc
      241 cgttcccatg aacaggaaca tccagcccat ctcgtgcgcc cccatgccca gccggtcgcg
      301 cagcagcagc gggaaggtcg tctcgagcgc cgccatggcg aacggcaccg tgaaggcgac
      361 gccgtagaag cgcaggagcg ggctgccgag cgccttccag ggggccggca ggtggaacca
      421 ggcgccatcg gaggcgggcg tcggtcgctg caggctctcc ggcaggagca tcgccgcggc
      481 gagaccggtg acgatcatca ggagaccggc gacgaggaac ggcgtggcgt gtccgaaacg
      541 ggcgagcgcc ccgccgatgc cggggccgaa gatgaagccc agccccatcg ccgcccccat
      601 cgcccccatt ccgtggccgc ggcgcttctc gtcggtgacg tcggcgacgt acgccatcgc
      661 ggtcggcagt gccgccgacg acatgagccc ggacagtcca cgcgcgaggt acagcgtcat
      721 caggcgatcg ccggcgccga acaggagcag gcccgcgccg ccccccagaa gccccgcgat
      781 cagcaccggc cgccgcccga tccgatcgga gaggcgcccc cagatcggcg cgaacaggaa
      841 ctgcatcagg gaatagaggg tgaacaggag cgcgatctcg atcgaggtcg cctgcagctc
      901 cgccgcccgg taggcgatgt tggggatcac gatcccgacc ccgagcatca ccaggaacag
      961 ggcggagaag aggatgagga gggctcggcg atccggcatc gcggcggtcc gcgatgatag
     1021 gggggggatc gggggctgtc aacaacgccc gctctcggtc tccatggtct gggacagcgg
     1081 ccaacaggac ccgttggtca cggcctcggt tctcccctct cgtcggcgtg acgcttcgag
     1141 ggggaggcga ttccggcgtg tcacgtgcat gtggcagact cgggggtatg gagagagcgg
     1201 ttgacgttct catcgtcggc ggaggggtga tcggcctggc gatcgcgagg gaggcggcgc
     1261 gatcggggat gtcggtgcgg ctgctcgagc gcggagagac cgggggggag gcgaccggtg
     1321 cgtcggcggg gatgctcgcc gcgcagctgg aggcccacga gccgggaccg ctgctgtcgc
     1381 tgtcactcga aagccggacg ctctaccccg ccttcctgcg ggcgctcgag agcgagaccg
     1441 gcgagtcggt cgacctgcga cgcgaggggg cgctggtgat cgccctcgac gaggcggcgc
     1501 gtgaggcgat cgcgcgctcc gcgtccgcgc agcgccgcat ggggctcgac gtgaagacgc
     1561 tcgacgccgt ggaggtccgc gccctcgagc ccctggtctc gggggatacc cgcggcggga
     1621 tgcacctgcc gcacgagcac acggtcgatc cgcgacgcct ggcggaggcg ctgcggcgcg
     1681 cggcggaggc ggccggcgcg gagatttgca ccggcgccga ggcgaccgcc atcctcatgg
     1741 agcggaagac ggcgcggggc gtgcggctcg cggacgggcg ggatcagcgg gcgcgggagg
     1801 tggtggtcgc cgccggcgcc tgggccggcg ccctccaggc agaaggaatc gcggtgcctc
     1861 cctcggagcc ggtacgcggg cagatgctgt gcttcgccgc gccggggatc gtccggcacg
     1921 tcgtcgcgtc ggaggaggtc tatctcgtcc cgcgaagcga cgggcgcctg ctcgcgggaa
     1981 gcacgatgga gcgggccggc tacgatcgga gcgtcaccgc gtcggggatg gcggggatcg
     2041 cagcggccgc catccggctc gtgccgcgcc tcggtcacgc ggccttccac tccgcctggg
     2101 cggggctgcg tccgggcgcg ccggacgggc tgccggtcct cggtcgcgcg gcgccggggc
     2161 tgctgtatgc gtgcggacat ctccgcaacg gcatcgtgct gactccgatc accgcggcga
     2221 tcgtggggcg gctcctgcgc ggagaggcgg cggggttcga tctgacgccc tacgacccgc
     2281 ttcgcttcgg cccggtgcag ggctgacgac tacttcttgc gggcggcctt ctggcgccgc
     2341 tcgtgctcgg cgaggaagtc gaccttgagc ttctcgacga gcttctcgcg gacgtcgtag
     2401 aggcgcttcg ccttgcgcgg cttgacccgc gccagcgcgt aggcggtcat cagcggcgcc
     2461 gcgatcgtcg agtcggtgta gacgacgacc gagtcggtca gcttgtcggg atcgaccttc
     2521 ccccacgaga ccgcctcgga aggcgtcgcc cccgagagcc cccctgtgtc ggggcgcgcg
     2581 tcggtgatct ggatgtagta gtcgtgcccg cggtcggtga gcccgaggat ctcctggatc
     2641 tgcggctcgg tctggagcac gaagttcttc ggcgagccgc cgccgagcat caggagaccg
     2701 ctcttcccgt cgccgttctt cgccgcgtag acgagggcgg cggtctcgtt gacgtcgcgt
     2761 gtcacgtcga tgcgcggacc gcagccgcgc atcgccagct cggcgacgtt catgccgatc
     2821 gacgagtcgc caggggaaga gacgtaacag gggacgccgg cgcggtaggc ggcggtcagc
     2881 acgcaggtgt cctcgacccc cagctccttc tcgcgcgccg cgaggtactt gccgagcaga
     2941 tagtgcagct cggcggtccc catctccttg ttgaacggct tctccgccat gatggtgcgc
     3001 aggtagttgt cggtgtcgag cagcacgttg tagtcgaaca ggatgtcgta ga
//
LOCUS       DASRIQ010000110         6228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3437294, whole genome shotgun sequence.
ACCESSION   DASRIQ010000110 DASRIQ010000000
VERSION     DASRIQ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3437294"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFD06_02610"
     CDS             complement(<1..328)
                     /locus_tag="VFD06_02610"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HZN02458.1"
                     /translation="MTSESPPGRLSRRRLHGAFKELHRTLRTEGDTPGRQAAAVALGT
                     FVGCTPIYGTHLVLCLALARLFRLNRLLTYLAAHINNPLTLPWLLALSYGLGHRFVQG
                     AWPPFSL"
     gene            complement(325..1149)
                     /locus_tag="VFD06_02615"
     CDS             complement(325..1149)
                     /locus_tag="VFD06_02615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN02459.1"
                     /translation="MTEAAARPLSAPGRAPLFVRLSAALHVAGAGVCLVAPRAWPLVV
                     ATIIPDHLLLAWGSLRPRSALVGPNLRRLPDRAAENEVALTFDDGPDPRTTPRVLDLL
                     DARRARATFFCVGRKAEQHPDLVREIARRGHRVENHSFGHAGTFCCWSPDAQAGDIDR
                     AQATLARLAGAVPRYFRAPAGLRSPWLDGVLRSRGLTLVSWTRRGLDTISRRPEAVAR
                     RLTRGLAAGDILVLHDGGGAVGPEALPRLLDAIESARLRSVPLPDPEPVDAAGAAA"
     gene            complement(1142..2614)
                     /locus_tag="VFD06_02620"
     CDS             complement(1142..2614)
                     /locus_tag="VFD06_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZN02460.1"
                     /translation="MRTPRTPDPIVTAFDRLARRRGDRPLVVARDRTVTVVGVDALAR
                     VAAEALAEAGLPPRSAVGLCAPNGAGFLAALLACRRTGHPAALLDAAAPAREIRDTAR
                     RLGFGGLLRVEPRDGARRFRVTRLEASRPPEAWHERVLGDAAVIKMTSGSSGRPRGVA
                     CSSAALLADDAAIRAAMGIGATDRLVAAVPFAHSYGLSSLVIPALTEGLPLLLPESGA
                     PLAPWIAAQELQGTVLPTVPAFVAGLAALQDAPPWPETLRLMITAGAPLRPDTARRLR
                     RRFGRPAHVFYGASEVGGIAFDASGDAGERGTVGTPLPGVRVTIETADGDLGADGGGV
                     PGAGRLVVRSAAAALSYLPEPDPALAKGRFRTSDLARRDGREIVLLGRLDALINVRGR
                     KIDPQEVERHIARLEHVEEVVVHAVHGPGDAEPRVRAVVACPSGRLSGVMVRHWCRGR
                     LGDHKVPREILLVPALPLTPRGKIDRDAVSRLPSRPSSDD"
     gene            2682..3926
                     /locus_tag="VFD06_02625"
     CDS             2682..3926
                     /locus_tag="VFD06_02625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZN02461.1"
                     /translation="MSPPAARPVRVAVTGLGAVTGFGWGVDALAAGLASGRSAIGPFD
                     LFDAARYATQIAAQVPAPPASLRRLPGWAAWSRADRFGVAAAREAIASAGLPFDAATG
                     RGTGVFFGGSTGGLLESERLYEAHRDGTLARAPRGPIRRHPVQRPAEAVARALGADGP
                     VETVCSACASGTLAIAAALDAVRQGEVRLALAGGADSLARVTYGGFNALRAVDARPCR
                     PFRGDRAGMSLGEGAAVLVLERRDAAETRGATILAEILAGGASADAHHMTAPDPRGLG
                     AVRAIARALEEGGRSPSDIDLVNTHGTGTPLNDRAEYAALRSVLQERAPVVPLAVSKG
                     SVGHLLGGAGAVEAVATVLALRSQRLQPVPGGGEHDPEMPVRLPAREEGARIRAALSI
                     NLGFGGVNGVLLLAPAGETPVR"
     gene            3923..5134
                     /locus_tag="VFD06_02630"
     CDS             3923..5134
                     /locus_tag="VFD06_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZN02462.1"
                     /translation="MIAITGLGTISACGVGRGPLLAALAAGRPRLTEVPPARGQRTGR
                     ARSAALVDPAVLETALRGRDTRRMSPPSRLALVAAAGALEEAGLAPTREPDPGLAVVA
                     STAFGPSSFTTRLFDQILDEGPAAASPALFTECVANAAVAQVDAVLRAGGPSYALAQG
                     EAGPLRAVARAASEIALGRARRALAGAVEEMPPLVHALLDRVGALAAPGGELGEQGRP
                     FDRDRRGFHAGEGATFLVLEEEGEARNRGASVAARLRAAGSAFDASAPTAGFGTGGAR
                     LARALRAGLDRAGLGPDGIDLVVSGASGSRAGDRLEAEMLRAVWGNRPLPPVLTPKAI
                     TGEYGGAFLAAALLAARGEAGGAQPWFGVADEACRIVPHRGPIPPGAKTVLVTSVAAG
                     GCASWIVLERP"
     gene            5131..5826
                     /locus_tag="VFD06_02635"
     CDS             5131..5826
                     /locus_tag="VFD06_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN02463.1"
                     /translation="MKGLRVAVAIPAYQAAATVGDVVRGTRLLCDEVLVIDDGSSDRT
                     SERAAAAGARVVRHARNLGKGRALRNAFEDLFDRGFDAVITLDADGQHLPEEIPRLLR
                     AAGEDGGADLVIGSRAHLFERMHPLRRTSNRSSSRAISFFAGRRFEDVQSGFRLYTRK
                     LIDRAGFPEPRFEAESAVVVRAVRLGLEVRCVRIALGEPDGRATSHYRPLVDSFRIAG
                     AVVRARLSPGRRV"
     gene            complement(5879..>6228)
                     /locus_tag="VFD06_02640"
     CDS             complement(5879..>6228)
                     /locus_tag="VFD06_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02464.1"
                     /translation="RSIARGECVPGGEAMGLPALMRDVYQVIGEYYRSVHAPHTSPVA
                     RRDRRNILGSFGLLPGVLFGVPVSLTSLHVLRQEWIARAGRWDALRADEPIPATTPAA
                     PGLEPDPALLPDA"
BASE COUNT          739 a         2332 c         2429 g          728 t
ORIGIN      
        1 gcaggctgaa cggcggccac gccccctgca cgaatcgatg gccgaggccg tacgacaggg
       61 cgagcagcca ggggagggtg agcgggttgt tgatgtgcgc ggcgaggtac gtgaggagcc
      121 ggttcaggcg gaagagccgc gcgagcgcga gacagaggac gaggtgcgtg ccgtagatcg
      181 gcgtgcagcc gacgaacgtg ccgagcgcca ccgcggccgc ctgacgcccc ggcgtgtccc
      241 cctcggtgcg cagcgtccgg tgcagctcct tgaacgcgcc gtgaagacgc cggcgcgaca
      301 ggcgcccggg cggcgattct gaggtcacgc ggcggctccg gccgcgtcca ccggctccgg
      361 atccggcagc ggcacgcttc tcagccgggc cgactcgatg gcgtcgagga ggcgcggcag
      421 cgcctccgga ccgaccgcgc ccccgccgtc gtgaagcacc aggatgtccc cggcggcgag
      481 cccgcgcgtc aggcggcggg cgaccgcctc gggacggcgg gagatcgtgt cgagcccgcg
      541 ccgcgtccag ctgacgagcg tcaggccgcg cgagcgcagc acgccgtcga gccaggggct
      601 gcgcagcccg gccggggcgc ggaagtagcg cgggacggcg ccggcgaggc gtgcgagcgt
      661 cgcctgcgcg cggtcgatgt cgcccgcctg cgcgtccggt gaccagcagc agaacgttcc
      721 cgcgtgcccg aaggagtggt tctccacccg gtgcccgcgc cgggcgatct cccggaccag
      781 atcgggatgc tgttccgcct tgcgcccgac gcagaagaag gtcgcgcgcg cccggcgcgc
      841 gtcgagcagg tcgaggacgc gcggcgtcgt ccgcggatcg gggccatcgt cgaaggtgag
      901 ggcgacttcg ttctccgccg cccggtccgg gaggcgccgc agattcggac cgacgagcgc
      961 gctcctcggc cggaggctgc cccaggcgag gagcaggtgg tccgggatga tcgtcgcgac
     1021 cacgagcggc caggcgcgcg gcgccacgag gcacacgccg gccccggcca cgtgcagcgc
     1081 agccgagagc cgcacgaaca gcggggccct gccgggagcg gagagcggcc tcgccgccgc
     1141 ctcagtcatc ggaggagggt cgggagggca ggcgcgacac ggcgtcgcga tcgatcttgc
     1201 cgcgcggcgt cagcggcagc gccgggacga gcaggatctc gcgcggcacc ttgtgatccc
     1261 ccagccgccc ccggcaccag tggcgcacca tcacgccact cagccggccc gagggacagg
     1321 cgacgacggc ccgcacgcgg ggctcggcat cacccgggcc gtgcaccgcg tgaaccacga
     1381 cctcctcgac gtgctcgagc cgcgcgatgt ggcgctccac ttcctgcggg tcgattttcc
     1441 gcccgcgcac gttgatgagc gcgtcgagcc gtccgagcag gacgatctcg cgcccgtcgc
     1501 ggcgcgccag gtcggaggtg cggaagcgcc cctttgcgag cgcggggtcc ggctccggga
     1561 ggtacgacag ggccgcggcg gcggagcgga ccaccagccg cccggcgccg ggaacgccgc
     1621 cgccgtccgc cccgaggtcg ccgtccgccg tctcgatcgt cacccgcacg cccggaagcg
     1681 gcgtcccgac cgtgccgcgc tccccggcgt ctcccgacgc atcgaaggcg atgccgccga
     1741 cctcgctcgc gccgtagaag acgtgggccg gacgtccgaa gcgccgccgc aggcgccgcg
     1801 ccgtgtcggg gcggagcggc gcgccggcgg tgatcatgag tcgcaaggtc tccggccacg
     1861 gcggggcgtc ctggagcgcc gcgaggccag ccacgaacgc cggcaccgtc ggcagcaccg
     1921 ttccctggag ctcctgcgcc gcgatccagg gcgcgagcgg cgcgccgctc tccggcagga
     1981 gcagcggcag gccctcggtc agcgccggga tcaccagcga cgacaggccg tagctgtgcg
     2041 cgaacggcac cgccgcgacc aggcgatcgg tggcgccgat gcccatcgcc gcgcggatcg
     2101 ccgcgtcgtc cgccagaagc gccgcggagg agcaggcgac gccgcgcggc cggccgctcg
     2161 agccggaggt catcttgatc accgccgcgt ccccgagcac ccgttcgtgc caggcctcgg
     2221 gcgggcggga ggcctcgagt cgcgtcaccc ggaagcgccg cgctccgtcg cggggctcga
     2281 cgcgcagcag tcccccgaac ccgagccggc gcgcggtgtc gcggatctcg cgcgccggcg
     2341 ccgcggcgtc gaggagcgcc gccgggtgcc cggtgcggcg gcaggccaga agcgccgcca
     2401 ggaacccggc gccgttcggg gcgcacaggc cgacggcgga gcgcggcggc aggcccgcct
     2461 cggcgagcgc ctccgccgcg acccgggcca gcgcgtccac gccgacgacc gtgacggtac
     2521 gatcgcgcgc gacgacgagc ggccggtcgc cgcggcggcg cgccagacga tcgaaggcgg
     2581 tgacgatggg atcgggcgtc cggggagtgc gcacgtggcg tgaattctat cccacgccga
     2641 tgcgtgggat aatttcgccg catgacaccc gtgtgcctcg cgtgagcccg ccggcggcgc
     2701 gtccggtccg cgtcgccgtg accggactcg gcgcggtcac cggattcgga tggggggtcg
     2761 atgcgctcgc cgcggggctg gcctccggcc ggagcgccat cggccccttc gatctgttcg
     2821 acgccgcccg ctacgcgacg cagatcgccg cgcaggtccc tgcgccgccg gcgtccctca
     2881 ggcgcctgcc cggctgggcc gcctggtcgc gcgccgatcg cttcggcgtc gccgccgccc
     2941 gcgaggcgat cgcgtcggcc ggcctgccgt tcgacgctgc gaccggccgg gggaccggtg
     3001 tgttcttcgg aggcagcacc ggagggcttc tggagagcga gcggctctac gaggcccacc
     3061 gtgacggaac tctcgcccgg gcgccgcgcg gcccgatccg ccggcacccg gtgcagcggc
     3121 ccgcggaggc ggtggcgcgc gcgctcggcg ccgacggccc ggtggagacc gtctgctctg
     3181 catgcgcctc ggggacgctg gcgatcgccg cggcgctcga cgcggtgcgc cagggagagg
     3241 ttcggctggc gctcgccggc ggggccgaca gcctggcgcg cgtcacctat ggagggttca
     3301 acgcgctgcg cgcggtcgat gcgcgccctt gccggccgtt ccgcggggat cgcgccggga
     3361 tgtcgctcgg cgaaggggcg gcggtgctcg tcctcgagcg ccgggacgcg gccgagacgc
     3421 gcggcgcgac gatcctcgcg gagatcctgg ccggcggcgc ctccgcggac gcgcaccaca
     3481 tgaccgcccc ggatccgcgc gggctcgggg cggtgcgggc gatcgcgcgg gcgctcgagg
     3541 agggcgggcg cagtccgtcc gacatcgacc tcgtgaacac gcacggcacc gggacgccgc
     3601 tcaacgatcg cgcggagtac gccgcgctcc gcagcgtcct ccaggagcgg gcgcccgtgg
     3661 tgccgctcgc cgtgtccaag gggagcgtcg ggcacctgct cggcggtgcc ggggcggtgg
     3721 aggcggtcgc gacggtgctc gccctccgga gccagcgcct gcagccggtc cccggcgggg
     3781 gcgaacacga tccggagatg ccggtccgcc ttccggcgcg ggaggagggg gcgcggatcc
     3841 gcgccgcgct ctcgatcaac ctcgggttcg gcggcgtcaa cggcgtcctc ctcctggcgc
     3901 cggccgggga gaccccggtc cgatgatcgc gatcaccggg cttgggacga tcagcgcctg
     3961 cggcgtcggg cgcggcccgc tcctcgccgc cctcgccgcc ggtaggccgc gtctcaccga
     4021 ggtgccgccg gcccgcgggc agcgcacggg ccgggcgcga agcgccgccc tggtcgatcc
     4081 ggcggtcctc gaaaccgcgc tgcgcggccg ggacacgcgg cgcatgagcc cgccgtcccg
     4141 cctggccctc gtcgccgcgg ccggcgccct cgaagaggcg ggtctcgcgc cgacgcgcga
     4201 gccggacccg ggtctcgcgg tcgtcgcctc caccgccttc ggtccgtcct cgttcacgac
     4261 ccggctgttc gatcagatcc tcgacgaggg gccggccgcc gcgtcgcccg ccctgttcac
     4321 cgagtgcgtc gccaacgcgg cggtggcgca ggtcgatgcc gtcctgcgcg ccggtggtcc
     4381 gagctacgcg ctggcgcagg gggaggcggg gccgctgcgc gccgtggccc gcgccgcgtc
     4441 ggagatcgcg ctcggacggg cgcgccgagc cctcgcgggg gcggtggagg agatgccgcc
     4501 tctggtgcac gcgctgctcg atcgcgtcgg ggcgctggcg gcgccgggtg gtgaactggg
     4561 agagcagggg cggcccttcg accgggatcg ccgtggattc catgcggggg aaggggccac
     4621 gttcctcgtc ctcgaggaag aaggggaagc gcggaaccgc ggcgcctccg tcgccgcgcg
     4681 gctgcgtgcg gcgggatcgg cgttcgacgc gagcgcaccg acggcgggat tcgggaccgg
     4741 cggtgcccgg ctcgcccggg cgctccgcgc cgggctcgat cgcgccgggc tcggccccga
     4801 cgggatcgac ctcgtcgtgt cgggggcctc cggcagccgc gccggtgacc ggctcgaagc
     4861 ggagatgctc cgcgctgtgt ggggaaaccg gccgcttccg cccgtcctga cgccgaaggc
     4921 gatcaccgga gagtatggcg gggcgtttct cgccgcggcg ctcctggccg cgcgggggga
     4981 ggccggcggg gcacagccct ggttcggcgt cgccgacgag gcgtgccgga tcgtcccgca
     5041 ccgcgggccg atccctccgg gcgcgaaaac ggtgctggtg acgagcgtcg ccgccggtgg
     5101 ctgcgcctcc tggatcgtgc tggagcggcc gtgaagggac tccgcgtcgc cgtcgccatc
     5161 cccgcctacc aggccgccgc caccgtcggc gacgtcgtgc gcgggacgcg cctcctgtgc
     5221 gacgaggtgc tggtgatcga cgacggctcg agcgaccgca cctccgagcg cgcggccgcg
     5281 gccggtgccc gcgtcgtccg gcacgcgcgc aacctcggca aggggcgggc gctgcggaac
     5341 gcgttcgagg atctcttcga ccgcggcttc gacgccgtca tcacgctcga cgccgacggc
     5401 cagcacctgc cggaggagat cccgcgcctg ctgcgtgcgg cgggggagga cggcggcgcc
     5461 gatctcgtga tcggcagccg ggcgcacctg ttcgagcgga tgcacccgct gcggcgcacg
     5521 agcaaccggt cctcgtcgcg cgccatctcg ttcttcgcgg ggcggcggtt cgaggacgtg
     5581 cagagcggct tccgcctcta cacccgcaag ctcatcgacc gggccggctt ccccgagccc
     5641 cgcttcgagg cggagagcgc ggtcgtggtg cgcgccgtgc ggctcggcct cgaggtgcgc
     5701 tgcgtccgga ttgccctcgg cgagccggat ggccgcgcca cgagccacta ccggccgctg
     5761 gtcgacagct tccgcatcgc cggcgccgtg gtgcgcgccc ggctttcgcc cggccgacgc
     5821 gtctaggaga ctgtccaaag ctccgggcgg acccgctccc gcgccggcgg cttccggctc
     5881 aggcgtccgg gagcagcgcc ggatccggtt cgagacccgg cgccgccggt gtcgtcgcgg
     5941 ggatcggctc gtccgcgcgc agcgcgtccc agcggcccgc gcgggcaatc cactcctgcc
     6001 gcaggacgtg cagggaggtc agggagaccg ggacaccgaa cagcacgccg ggcaggagcc
     6061 cgaacgatcc gaggatgttg cgccggtcgc gccgcgccac cgggctcgtg tgcggcgcat
     6121 gcaccgaccg gtagtactcg ccgatgacct ggtagacgtc gcgcatcagt gccggcagcc
     6181 ccatcgcttc tccgcccggc acgcactccc cgcgcgcgat gctccgga
//
LOCUS       DASRIQ010000111         4942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3163614, whole genome shotgun sequence.
ACCESSION   DASRIQ010000111 DASRIQ010000000
VERSION     DASRIQ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3163614"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFD06_02645"
     CDS             complement(<1..911)
                     /locus_tag="VFD06_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZN02465.1"
                     /translation="MSPLDYAVLAGTLLAIATYGAWRTRGSRDLEGYLRADHALKWGT
                     IGLSVMATQASAITFLSIPGFGYEKGLMFVQNYLGLPIALVVVSALFIPVYYRLKVYT
                     AYEYLERRFDLKTRQLGAMLFLLQRGLAAGITIYAPAIILSNLLGWNLRLTIVVAGLI
                     VIAYTVSGGSRAVSLTQRHQLTVALAGILVAFVLLVAHVTRQVPFGTALGIAGQMGRL
                     EAISFSFDPGTRYTFWSGILGGCFLALSYFGTDQSQVGRYLGGTSVAASRFGLMFNAV
                     LKVPMQFFILFVGVMVCLFYQFQEPPLL"
     gene            complement(908..1063)
                     /locus_tag="VFD06_02650"
     CDS             complement(908..1063)
                     /locus_tag="VFD06_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02466.1"
                     /translation="MTEPGAPLPAADPTGDPPPPALHSWGRVYALVLGWLGIMVVLMY
                     AFTLRYR"
     gene            complement(1060..3567)
                     /locus_tag="VFD06_02655"
     CDS             complement(1060..3567)
                     /locus_tag="VFD06_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZN02467.1"
                     /translation="MRGGLGPAGFCAAIGLAGWAGGAFAAPLVPPRPDAAAIQLALNK
                     LQVLESVLYVAAHPDDENTRLIACLVNGRLADTAYLSITRGDGGQNLIGPELGELLGV
                     IRSQELLAARRLDGGRQFFTRAIDFGYSKTAEETLRIWDRTQVLADVVRVYRQFQPDV
                     VINRFPYADRDTHGNHTASAILAKDAMVAAGDPKMFPEQLTTLKPWRPRRLLWNTSQW
                     FYDNKEDFKPETLLKVDVSGFSPLLGESFPELAARSRSMHRSQGFGSSGSRADVTEYL
                     EHVDGEKAQADLFDGIDTTWGRVPGGQAVGAILARAAKQFDPQNPPDSVPLLLEARRA
                     MEALPEGRWKSAKMAELDQVVVACLGLYLEAAAAEPTGVPGNKVSITVEAANRSPVNV
                     RIARVTVKEIRSDTSWDQTLAGRGSAKKTIEAALPAELPDSEPYWLRQRGTQGMFRVD
                     EAALIGQPENPPALSVVFGLEVGGTSLGVERPVVYKWNDPARGERYRPFEIVPAVTLE
                     VGEKVLLFPDAQPREVPVRLRAGSAVAGQVRLEAPAGWSVTPASREFSLSEKGATQSV
                     TFSVKPSGAPSAGALRAVAESEGRRFDRGLTIVEYDHIPTQTILPPAEARVVRLALQR
                     RGQEIGYVQGAGDQIPTGLRQIGYNVTELGQADLVPERLKRFDAVILGVRAYNTIDGI
                     RFRQAALFDYAKAGGTLIVQYTTPQEIKVDAPLPLPLKLSRERVSEEQAEVKVLVPES
                     PVVTRPNRIASSDFEGWVQERGLYFASEWDPAFQAILSSHDEGESPKNGGLLVAPYGK
                     GHVVYTGYSWFRQIPAGVPGAIRLFVNIISLGGPSAS"
     gene            complement(3589..4785)
                     /gene="bshA"
                     /locus_tag="VFD06_02660"
     CDS             complement(3589..4785)
                     /gene="bshA"
                     /locus_tag="VFD06_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HZN02468.1"
                     /translation="MGERSDSPRGTDRSARFGEALLKLAIVCYPSVGGSGILASQLGQ
                     QLGRRGHEVHFVAYGPPFRLDPNAPNVRFHPVRINEYELFRYPDYTLPLSVALADVSR
                     EFGLDLIHAHYAVPHAVAAYLARRMLGAASPRLITTLHGTDTTLLGKDPSYRPIIRHA
                     IAASDGVTAVSAFLRDETRQIFGIDREIDVVHNFFAPQKPKRSRSEVRASLGVGSETV
                     ALHMSNLRSGKRIEDLLAALARVRNGKRLRLLVLAGGDFAPYRPLVESLGIQDRVIVR
                     GAVPDVEDYLAASDFGVYTSESESFGLSILESMFHGHPVLATRAGGVPEVVVDGETGF
                     LLPPGDVGGFAARMEEMAEDPERFRAMGERGRARAEALFSADRIVDRYLEVYRRVLSE
                     PERSGL"
     gene            complement(4719..>4942)
                     /locus_tag="VFD06_02665"
     CDS             complement(4719..>4942)
                     /locus_tag="VFD06_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02469.1"
                     /translation="RLLVDVSDAFEAWKEAMRCHASQMKTRDYVDLLVARARVLGAQV
                     GVEHAMAVWANDPIRLAALTDLRDSGRRF"
BASE COUNT          784 a         1711 c         1750 g          697 t
ORIGIN      
        1 aggagcggtg gctcctggaa ctggtagaag aggcagacca tcacaccgac gaagaggatg
       61 aagaactgca tcggcacttt gaggacggcg ttgaacatca ggccgaaccg gctggcggcg
      121 accgaggtcc ctcccaggta ccgccccacc tgcgattggt ccgttccgaa gtacgagagc
      181 gccaggaagc agccgccgag gatgccggac cagaaggtgt agcgcgtgcc ggggtcgaag
      241 gagaaggaga tcgcctcgag gcgccccatc tgaccggcga tcccgagcgc ggtgccgaat
      301 ggaacctgcc gggtcacgtg ggccaccagg agcacgaagg cgaccaggat ccccgcgagg
      361 gccaccgtca gctggtgccg ctgcgtcagg ctgacggcgc gcgagccccc ggagacggtg
      421 taggcgatga cgatgagccc cgccaccacg atggtgagcc gcaaattcca accgagcagg
      481 ttcgacagga tgatggccgg ggcgtagatg gtgatgccgg cggcgagccc gcgctgcagc
      541 aggaacagca tcgcgccgag ctgccgcgtc ttgagatcga agcggcgctc caggtactcg
      601 taggcggtgt aaaccttgag ccggtaatag accgggatga acagcgccga aaccaccacg
      661 agggcgatcg gcaggccgag atagttctgg acgaacatca accccttctc gtagccgaac
      721 ccggggatcg acaggaaggt gatggcgctc gcctgcgtcg ccatgaccga cagcccgatc
      781 gtcccccact tcagggcgtg atcggcgcgc aggtaccctt ccagatcgcg gctgccgcgc
      841 gtgcgccagg cgccgtaggt ggcgatcgcc agcagcgtgc cggcgaggac agcgtagtcg
      901 agggggctca ccggtagcgc agcgtgaagg cgtacatcag gacgaccatg attcccagcc
      961 acccgaggac gagggcgtac actcgtcccc aggaatgcag ggccgggggt ggcggatcgc
     1021 ctgtcgggtc ggccgcgggg aggggcgcgc cgggttcggt cacgaggccg agggccctcc
     1081 gagcgaaatg atattgacga acagccggat cgcccccggc acgccggccg ggatctgccg
     1141 gaaccaggag tagccggtgt agaccacgtg ccccttgccg tagggcgcga ccagcaggcc
     1201 gccgttcttc ggggattccc cttcgtcgtg gctggagagg atcgcctgga aggccgggtc
     1261 ccactcgctc gcgaagtaga ggccacgctc ctgcacccac ccctcgaagt cggaggaggc
     1321 gatgcggttc ggtcgggtga ccaccgggga ctcggggacg aggaccttca cctccgcctg
     1381 ctcctcgctc acccgttcgc gcgacagctt cagcggaagc ggcagcggcg cgtccacttt
     1441 gatctcctga ggcgtggtgt actgcacgat gagcgttccc cccgccttgg cgtaatcgaa
     1501 cagggccgcc tggcggaagc ggatcccgtc gatcgtgttg taggcgcgca cgccgaggat
     1561 caccgcgtcg aagcgcttga gccgctcggg gacgagatcg gcctgcccga gctcggtgac
     1621 gttgtagccg atctgccgca gcccggtcgg gatctggtcg cccgccccct gcacgtagcc
     1681 gatctcctgt ccgcgccgct gaagggccag gcggacgacg cgcgcctcgg cgggcggaag
     1741 gatcgtctgc gtcgggatgt gatcgtattc gacgatcgtc agaccgcgat cgaaacggcg
     1801 gccctccgac tcggccacgg cgcgcagcgc ccccgcggag ggcgcccccg acggcttcac
     1861 gctgaacgtc accgactggg tggccccctt ctcggacagg ctgaactcgc gcgacgcggg
     1921 ggtgacgctc cagccggcgg gcgcctcgag gcggacctga ccggccacgg cgctcccggc
     1981 ccgcagccgc accggcacct cgcgcggctg cgcgtccggg aacagcagga ccttctcgcc
     2041 gacctccagg gtcaccgccg ggacgatctc gaacggacgg tagcgctcgc cgcgcgccgg
     2101 gtcgttccac ttgtagacga ccggacgctc cacgcccagg gaggttcccc ccacctccag
     2161 cccgaagacg acgctcaggg ctggcgggtt ctcgggctgg ccgatgagcg cggcctcgtc
     2221 cacccggaac atcccctgcg tgccgcgctg ccgcagccag tacggctccg agtccggcag
     2281 ttcggcgggg agcgccgcct cgatcgtctt cttcgcggac ccgcgaccag cgagcgtctg
     2341 gtcccacgac gtgtcgctgc ggatctcctt gacggtgacc ctggcgatgc ggacgttcac
     2401 cggagaccgg ttggccgcct cgacggtgat cgacaccttg ttcccgggca cgccggtcgg
     2461 ctccgcggcg gccgcctcga ggtagaggcc gaggcaggcg acgaccactt ggtcgagctc
     2521 ggccatcttg gcgctcttcc agcgcccctc cggcagggcc tccatcgcac gccgcgcctc
     2581 gaggaggagc ggcaccgagt cgggcggatt ctgcggatcg aactgcttcg cggcgcgggc
     2641 caggatcgcc ccgaccgcct gtccgcccgg gacgcgcccc caggtggtgt cgatcccgtc
     2701 gaacagatcc gcctgcgcct tctcgccgtc gacgtgctcg aggtactccg tgacgtcggc
     2761 gcgcgagccg ctcgacccga acccctggct gcggtgcatg ctgcggctgc gcgccgccag
     2821 ctccgggaac gactcgccga ggagggggga gaagccgctc acgtccacct tgaggagcgt
     2881 ctccggcttg aagtcctcct tgttgtcgta gaaccactgc gaggtgttcc acagcagccg
     2941 gcgaggccgc cacggcttga gggtcgtgag ctgctccggg aacatcttcg ggtcccccgc
     3001 ggccaccatc gcgtccttcg ccaggatcgc cgaggcggtg tggttgccgt gcgtgtcccg
     3061 gtcggcgtac gggaagcggt tgatgacgac gtccggctgg aactggcggt agacgcgcac
     3121 cacgtccgcc aaaacctggg tgcggtccca gatgcgcagc gtctcctccg cggtcttcga
     3181 atagccgaaa tcgatggcgc gcgtgaagaa ctgccggccg ccgtcgagac ggcgcgccgc
     3241 cagcagctcc tgcgatcgga tgacgccgag cagctcgccg agctcggggc cgatgaggtt
     3301 ctgtccgccg tcgccgcgcg tgatcgagag gtaggcggtg tcggccaggc gcccgttgac
     3361 gaggcaggcg atgagccggg tgttctcgtc gtcgggatgg gcggcgacgt acagcacgct
     3421 ttccagcacc tggagcttgt tgagcgcgag ctggatcgcc gcggcgtcgg ggcgcggcgg
     3481 cacgagcggg gcggcgaacg cgccgccggc ccagccggcg agcccgatcg ccgcgcagaa
     3541 accggcgggt ccgagtcctc ctctcatcgc tccctgtccc ccttctggct agaggccgct
     3601 ccgctccggc tcggagagga cgcggcgata gacctcgaga taccgatcga cgatgcgatc
     3661 ggcggaaaac aatgcctcgg cgcgcgcccg cccgcgctcc cccatggcgc ggaaacgctc
     3721 cgggtcctcc gccatctcct ccatccgggc ggcgaacccg ccgacgtcgc ccggcggcag
     3781 gaggaacccg gtctcgccgt ccacgaccac ttccggaacg ccgccggcgc gcgtggcgag
     3841 gaccggatgg ccgtggaaca tgctctcgag gatcgacagg ccgaaggatt cgctctccga
     3901 cgtgtagacc ccgaagtcgg aggcggcgag gtaatcctcg acgtccggca cggcgccgcg
     3961 gacgatgacc cgatcctgga tccccagcga ctcgacgagc ggccggtagg gcgcgaaatc
     4021 cccgcccgcg aggacgagga gccgcagccg cttcccgttc cgcacgcgcg ccagagccgc
     4081 cagcaggtcc tcgatccgct tcccggatcg gaggttcgac atgtgcagcg ccaccgtctc
     4141 ggagccgacg ccgagcgagg cccgcacctc gctccgcgac cgcttcggct tctgcggcgc
     4201 gaagaagttg tgcaccacgt cgatctcccg gtcgatgccg aagatctgcc gcgtctcgtc
     4261 gcgcaggaag gcggagacgg cggtcacgcc gtcggacgcc gcgatggcgt gccggatgat
     4321 cggccggtac gacgggtcct ttcccagcaa ggtggtgtcg gtgccgtgca gcgtcgtgat
     4381 gaggcgcggc gaggcggcgc cgagcatgcg gcgcgccagg taggcggcga ccgcgtgcgg
     4441 cacggcgtag tgcgcgtgga tgaggtccag cccgaactcc cggctgacgt cggcgagcgc
     4501 caccgacagc ggcagggtgt agtcgggata gcggaacagc tcgtactcgt tgatccgcac
     4561 cgggtggaag cggacgttcg gcgcgttcgg atcgagccgg aacggcggcc cgtacgcgac
     4621 gaagtggacc tcgtgtccgc ggcgtccgag ctgctgcccg agctgcgacg ccaggatgcc
     4681 gctgccgccg accgagggat agcagacgat ggcgagcttc agaagcgcct ccccgaatcg
     4741 cgcagatcgg tcagtgccgc gaggcgaatc ggatcgttcg cccacaccgc catggcgtgc
     4801 tcgacgccga cctgcgcccc gaggacccgg gcgcgcgcca cgagcagatc gacgtagtcg
     4861 cgcgtcttca tctgggaggc atggcagcgc atcgcctcct tccacgcctc gaacgcgtcc
     4921 gacacgtcca cgagcaggcg gg
//
LOCUS       DASRIQ010000112         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_45471, whole genome shotgun sequence.
ACCESSION   DASRIQ010000112 DASRIQ010000000
VERSION     DASRIQ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45471"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFD06_02670"
     CDS             complement(<1..188)
                     /locus_tag="VFD06_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02470.1"
                     /translation="MADEYLDVIRNEARLDALRRTCLLDSPPEEAFDRLTRMATLVLR
                     VPMALVSLVDRDRHYFKS"
     gene            complement(230..364)
                     /locus_tag="VFD06_02675"
     CDS             complement(230..364)
                     /locus_tag="VFD06_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HZN02471.1"
                     /translation="MFFGALGATTRPVPESAAYPHLARVKRAYDSDNLFRMIQNIKPA
                     "
     gene            complement(410..1300)
                     /locus_tag="VFD06_02680"
     CDS             complement(410..1300)
                     /locus_tag="VFD06_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02472.1"
                     /translation="MIGRTSALILAFLLSGCAALRLGSEPVDKIRTDPCAGGSALPAK
                     LLEPGGTLLFGEIHGTQELPGFFVEAVCWASGALPVQVGLEVSKSEQERIETFLASAG
                     AAADVEAMTAGPFWTGFQDGRKSRAMVALLDRLRRLRKEGRPIEVFLFVGDSEDMAEH
                     DRMMADAIAARVRAHPEALTMVLTGNIHAQKNKGVPWNPDLVPMGWYLADAGVRVIAL
                     NHSTPAGTTWSCYPGPPLDCGRYPMRATAPLPSGRTIGIELLAEPTKEGFDGLYALPT
                     LTASPPALQAVTAATEESRP"
     gene            complement(1365..>2746)
                     /locus_tag="VFD06_02685"
     CDS             complement(1365..>2746)
                     /locus_tag="VFD06_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02473.1"
                     /translation="WGPGVSGNTWLLAGGSFGYAYPAQFLSTGQIHNVARWDGTAWYR
                     FGRYPDDGVAGSHYFGGPQVSDFLEFDVGLGPSLYMAGGYSEAGGAPANSMARWADGF
                     SPLGAGLTTLTYAGTAQAAAGFQGQIYVAGAFNHAGPDFARNLARWDGSAWSSAGGPL
                     IDGAYVEGLHSANLGNGTLLYMGGFFLELSNPGGGPTQLARHIASFDGQNWRKLGAGL
                     GPDSNDEEDPEERPHGMVMEHRLEADGNCLYVGGQFDTAGAITASSIARWCCTKVPYH
                     PSELINAYLVTAQTPFSFTDNDGDGLPESIACDDDVEIPVLPGTELQACGLRFVLGTE
                     RNDDLHLETSTTPVVVFGGPGRNQVLGSRFDDLILGGSDDETLDGGAGDDQIYGGFGD
                     DTLFGDLGDDRLIAGPGDDRLDPGLGLNAVDGGEGTDTAFVQPADEVVDVETVTFLGG
                     GGRSRTRRK"
BASE COUNT          455 a          953 c          888 g          450 t
ORIGIN      
        1 tgactcttga agtaatgccg atcccggtcg accagcgaga cgagggccat cggcacgcgg
       61 aggacgagcg tcgccatgcg cgtcagtcga tcgaaggcct cctcgggcgg ggaatcgagg
      121 aggcacgtcc gccgcagcgc atcgaggcgc gcctcgttcc ggatgacatc gagatactca
      181 tccgccattg gtcacgcagg tgggtctcag ctcgagcacg gcgccttcct caggccggct
      241 tgatgttctg gatcatccga aagagattgt ccgaatcata cgcccgtttg acccgtgcca
      301 ggtgcgggta cgcggcggac tcagggaccg gccgcgtggt ggcgccgagg gcgccgaaga
      361 agatctcggc ctgcggcgac ggtcgccggc tctcatggtc ggccgtggct cacggccggc
      421 tctcctcggt ggcagcggtc accgcctgca gcgccggtgg cgatgcggtg agggtcggca
      481 gcgcgtacag gccgtcgaag ccttccttcg tcggctccgc cagcagctcg atcccgatcg
      541 tccgcccgct cggcagtggc gccgtcgccc gcatgggata cctgccgcag tcgagcggcg
      601 ggccgggata gcacgaccag gtcgttcccg ccggcgtgga gtggttgagc gcgatcaccc
      661 tcacacccgc gtccgccagg taccagccca tcggcacgag atccggattc cacggcacac
      721 ccttgttctt ctgcgcatgg atgttgccgg tcagcaccat cgtcaaggcc tccgggtgcg
      781 ctcgaacgcg cgccgcgatc gcgtccgcca tcatcctgtc gtgttcggcc atgtcctccg
      841 agtcgccgac gaacaggaag acttcgatcg gacgcccctc cttgcgcagt cggcggagac
      901 gatcgagcag cgcgaccatc gcacgactct tccttccgtc ctgaaagccg gtccagaatg
      961 gaccggcggt cattgcctcg acatcggccg cggcgccggc cgatgcgagg aaggtctcga
     1021 tacgctcctg ctccgacttc gagacttcga ggccgacctg cacaggcaga gcgccggagg
     1081 cccagcacac cgcctcgacg aagaaacccg gcagctcctg cgtgccgtgg atctcgccga
     1141 agagcagggt gccccccggc tccaggagct tcgccgggag cgccgaccct cccgcacacg
     1201 ggtcggtccg gatcttgtcc accggctccg atccgagccg cagcgccgcg cagcccgaca
     1261 acaggaaggc cagaatcagt gccgatgtcc gtccgatcat cggaggatct cctcgaggtc
     1321 gacggcggcg aggatacacg tcctccgacg tggggatcag cggatcactt ccggcgggtt
     1381 cgactccggc cgccgccacc caggaacgtc accgtctcta cgtcgaccac ctcatccgcc
     1441 ggttgaacga aggccgtgtc cgttccctcc ccgccgtcga ccgcattgag ccccagaccg
     1501 ggatcgagcc gatcgtcccc ggggccggcg atgagccggt cgtcgccgag atccccgaaa
     1561 agggtgtcgt cgccgaagcc tccgtagatc tgatcgtctc ccgcaccacc gtcgagggtc
     1621 tcgtcgtccg atccgccgag gatcagatcg tcgaacctgc tgccgaggac ctgattccgc
     1681 ccgggaccgc cgaacacgac gaccggcgtg gtcgaggttt cgagatgcag atcgtcgttg
     1741 cgctcggtcc cgagcacgaa ccggagtccg caggcctgaa gctcggtgcc gggaagaacc
     1801 ggaatctcca catcatcgtc gcaggcgatc gattccggca gtccgtcgcc gtcgttgtcc
     1861 gtgaagctga atggggtctg ggctgtgacg agataggcgt tgatcagctc gctcggatgg
     1921 tacgggactt tcgtgcagca ccagcgtgcg atcgatgacg ccgtgatcgc ccccgccgtg
     1981 tcgaactgac ctcccacgta caggcagttg ccgtcggctt cgaggcgatg ctccatgacc
     2041 atgccatggg ggcgctcctc gggatcttcc tcatcgttgg aatcgggtcc cagcccggct
     2101 cccagcttcc tccaattctg gccgtcgaag ctggcgatgt gccgggcgag ctgcgtcggg
     2161 cccccgccgg gattcgaaag ctcgagaaag aaaccgccca tgtagagcag cgtgccgttg
     2221 ccgagattgg cgctgtgaag gccctccacg taggccccgt cgatcagggg gccccccgcc
     2281 gaagaccatg cggagccatc ccagcgtgcg agattgcgcg caaaatcagg ccccgcatgg
     2341 ttgaacgcgc cggccacata gatttgaccc tggaaacccg cggccgcttg tgccgttccc
     2401 gcgtacgtca gtgtcgtgag tccggccccg agcgggctga acccatcagc ccagcgcgcc
     2461 atcgagttgg caggtgctcc gccggcttcg gagtatcccc ctgccatgta caaggacggg
     2521 ccgaggccga cgtcgaactc gaggaagtcg ctgacttgcg gaccgccgaa atagtgtgac
     2581 ccggcgacgc cgtcgtcggg gtacctcccg aaccggtacc aggccgttcc gtcccatcgc
     2641 gcgacgttat ggatctgccc ggtcgacagg aactgtgccg gataggcgta gccgaagctg
     2701 ccgccggcga gcagccaggt gttcccgctc acgccgggac cccagc
//
LOCUS       DASRIQ010000113         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2657981, whole genome shotgun sequence.
ACCESSION   DASRIQ010000113 DASRIQ010000000
VERSION     DASRIQ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657981"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..>2021
                     /locus_tag="VFD06_02690"
     CDS             135..>2021
                     /locus_tag="VFD06_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02474.1"
                     /translation="MRRIVIAAVVCSLVVGAGGASRAAYVNFESPHVHPIDLTPSGAR
                     FLAVNTPDALLEVFTVSPDGSLAPQASIPVGLEPVTVVARTDAEAWVVNHLSDTVSIV
                     DLDLGTVTRTIATGDEPTDVAFASGRAFVAVSNEDAVKVWSLADLTLPPDRVDLFGRD
                     VRALAASADGTKVYAVVLQSGNQTTIVNANVIWGSGIGLDPTRLAALGLNNIVCPNNN
                     QHPPYPPHPAGIPRNPTLPDPSGGTQPPVALIVKWDPATSKWRDEAGQDWTPCLPFRL
                     PDHDLFVIDAANPTTAGTTMVDHLGTSLFEVSVNPANGRIYVPHTEARNQVRFEHALG
                     VRGHVVDNRLAIVNPQAGNAVTLVDLNAHINRASDPASNLVERQASLSQPGMMVWNAA
                     GTTAWMTAIGSRKVFRLSQACLNGAGPNYGACVFGANRAAPDTVEVGEGPTGVALREG
                     AVPRLYVLDRFSNSIAVVDPSAMTKIGERALHDPSLPSMKVGRRHLYDGIDSSGHGDA
                     ACSSCHISGDRDGLAWDLGDPTGAFVPYGTSGDNVRFFPIDNPAIAAAHTGFDPEKGP
                     MTTQTLRGMLEPLHWRGDRPTMIRFNKAFVGLMGTADIGPIDGEPAGISAQRMTEFRR
                     FALDI"
BASE COUNT          301 a          729 c          693 g          298 t
ORIGIN      
        1 acgggcttgc gcctcagcgg gggacgctac ggcggcgacg gccgctgcgg tgcgacaccc
       61 tctccacgtt caccggcgga cgcgaggaat cggtcgttgt tcggcgcgga ccttggtggt
      121 agattccagc caccgtgcgc cgcatcgtca tcgctgccgt cgtctgctcg ctggtggtgg
      181 gcgcgggcgg ggcgtcgcgc gcggcctacg tcaacttcga atcgccgcac gtccatccga
      241 tcgacctgac cccctccggc gcgcggttcc tggccgtcaa caccccggac gcgctgctcg
      301 aggtcttcac cgtctcgccc gacgggagcc tcgcgccgca ggcctcgatc ccggtcgggc
      361 tcgagccggt gacggtcgtc gcccgcaccg acgcggaagc ctgggtcgtg aaccatctct
      421 ccgacacggt cagcatcgtc gacctcgatc tcggcacggt gacgcgcacg atagcgaccg
      481 gtgacgagcc gaccgacgtc gccttcgcct cggggcgcgc cttcgtggcg gtgtcgaacg
      541 aggacgcggt gaaggtgtgg agtctcgccg acctgacgct gccgcccgac cgcgtcgatc
      601 tgttcgggcg cgacgtgcgg gcgctcgcgg cgtcggcgga cggcaccaag gtgtacgccg
      661 tggtcctgca gtcggggaac cagacgacca tcgtcaacgc caacgtcatc tgggggtcgg
      721 ggatcgggct cgacccgacg cggctggctg ccctcgggct caacaacatc gtctgtccga
      781 acaacaacca gcacccgccc tatccgccgc atcccgccgg catcccgcgc aatccgacgc
      841 ttccggatcc gtccggcggg acgcagccgc cggtcgccct catcgtcaaa tgggatccgg
      901 cgacctcgaa gtggcgcgac gaagcggggc aggactggac gccgtgcctg ccgttccgtc
      961 tgccggatca cgacctgttc gtcatcgacg cggccaaccc gacgaccgcc gggacgacga
     1021 tggtcgatca cctcgggacg tctctcttcg aggtgtcggt caacccggcg aacggccgga
     1081 tctacgtgcc gcacacggaa gcccgcaacc aggtccgctt cgagcacgcg ctgggggtgc
     1141 gcgggcacgt ggtggacaac cgtctcgcca tcgtcaaccc tcaggccggc aacgcggtga
     1201 cgctcgtcga tctcaacgcc cacatcaacc gggcgagcga tccggcgtcg aacctcgtcg
     1261 agcggcaggc gagcctgtcg cagcccggga tgatggtctg gaacgccgcc ggcaccaccg
     1321 cctggatgac ggcgatcggc tcgcgcaagg tgttccggct gagccaggcg tgcctcaacg
     1381 gcgccggccc gaactacgga gcgtgcgtct tcggcgcgaa ccgggcggcg ccggacacgg
     1441 tcgaggtggg ggaggggccg accggcgtgg cgctgcgcga gggggcggtg ccgcgcctct
     1501 atgtcctcga ccgcttcagc aacagcatcg ccgtcgtcga tccctccgcc atgacgaaga
     1561 tcggcgagcg ggcgctgcac gatccgagtc tcccctcgat gaaggtcggg cggcggcacc
     1621 tctacgacgg catcgactcg tccggccacg gcgacgccgc ctgctcctcc tgccacatct
     1681 ccggcgatcg ggacggcctg gcgtgggacc tcggcgatcc gaccggggcg ttcgtcccct
     1741 acggcacgtc gggggacaac gtgcgcttct tcccgatcga caacccggcg atcgccgcgg
     1801 cgcataccgg gttcgatccg gaaaaggggc cgatgacgac gcagacgctg cgcggcatgc
     1861 tggagccgct ccactggcgc ggcgaccgcc ccacgatgat ccgcttcaac aaggcgttcg
     1921 tggggttgat gggaacggcc gacatcggcc cgatcgacgg cgagccggcc gggatctcag
     1981 cccagaggat gacggagttc cgccgcttcg ccctcgacat c
//
LOCUS       DASRIQ010000114         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1859154, whole genome shotgun sequence.
ACCESSION   DASRIQ010000114 DASRIQ010000000
VERSION     DASRIQ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859154"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..1153
                     /locus_tag="VFD06_02695"
     CDS             383..1153
                     /locus_tag="VFD06_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZN02475.1"
                     /translation="MALRLVRNVGPRAGESGPGKDATAAFLLINGRGGVVESAGWEAL
                     SLEPVPQSIPAADDARETEDPILRGIAGAVADARRGRRPSIRLVEVTLQRAHVFVIVA
                     APLPGAGRNERVAVTVAETGPPEAGGNEGKVIRQLGHDLRTPLTSISGAIELLQSGRL
                     GGLQEGQQKVLGLLEKGADALLKLINEATFPYRGSRDLASVLGVGVEELVAGGLEEGA
                     GQDADPLEPGDDGDETPGGAAPSGAGARRNRPRRERGR"
     gene            1150..1959
                     /locus_tag="VFD06_02700"
     CDS             1150..1959
                     /locus_tag="VFD06_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN02476.1"
                     /translation="MKRTILVADDSQAVRDILQMSLETLGYTVVLAEDGERAMATIQA
                     SHPDLIIADVMMPKVNGFQICRRVKSDPSTRRTPVILLTARSGQEDIFWGKDCGADDY
                     ITKPFKTQDLEQTIRRLMRGRDPNGTGPIDMDQQRRRRVAEGGQIVMLRWDPRAMDVF
                     RKKYGEIAFSQALDRLKEEAEAYLAERSEPGPVGVHVPVGLSVVVAGVASQAIVVGQE
                     LAVRLSRTALDLYDGDDRVQGCIRFRDPRSGREERLPLLSFSPRIDPDTLV"
     gene            1976..>3988
                     /locus_tag="VFD06_02705"
     CDS             1976..>3988
                     /locus_tag="VFD06_02705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN02477.1"
                     /translation="MIPRKLGRFEIVREIGRGAMGQVLLAHDPKIDRQVALKTLAPSP
                     GISEEEARETSQRFLREAQAAGKLLHPHIVTIFDVGEDAGLSFIAMEYIDGVTLERHA
                     RADSLLPPARLLELMTQAGGALDYAHQAKVIHRDIKPANLMVQDGNLKVTDFGLAKNP
                     SASLTQTGVLLGTPSYMSPEQIQGKDLDGRSDLFSLGVVLYELLTGVRPFEADSISTI
                     IYRVLYEDPRPAAAHNPALPPAVNAVLEKALAKDPARRYATGAALVADLRKAFSDLPA
                     ETLARPFPIPSPPPGGRRPSVPSMATRPGHGPMRPARPGGATARTARPPQPAPAPQPT
                     LLAHHPVKILALGLTVVLGIALFPRWANRSERRSARQSAGAVQLPEAGPAQAAGVGGV
                     PAAAAASGGRISVDVETKPAGGSVFLDDIPLAEPKVVLALSDPTTHAIRARSGCLEGT
                     AEKSAADLAALHGPLVLELRERREVVAIGSEPPGARIRIDGRDTGKTTPVEIEMDACR
                     ERTVDLRLDGHRRWTRTFTAGEDVEAVAGALSAVALEPIPRGTVALPRPKGWSGDVFL
                     GNARVGGAGEPIALDEGTYTLVVRNDKVFARTSVPVTVRANETTRPAVALPGLGSLTV
                     QAQPSNCKVYVDGTYVDVTPVLDLPIAAGAHDIKVVFVPTGAAKSKN"
BASE COUNT          577 a         1425 c         1450 g          536 t
ORIGIN      
        1 ctgtcgcgct cgccgccacg cgtgcgctcc agagcctgct cttcggcgtc gcaccggccg
       61 acggcgccac ctatgccggc gtggtcaccc tcgtggtcct catggtcgcc atggcggtgt
      121 attggccggc gcggcgggcc accgcagtcc agccctcggt gctcttgcgc agcgactaga
      181 gtaggcggac gtgcgaagcc gcggccggtc cccgcgggac actccctcca ccgcctccca
      241 gcatgagggt tgcctcctct cctttttgct tcgcgcgctc ccgaccttgc gcctggctcc
      301 ggcgctccta tattgatgac ggaatcgtcc tgagacctcg gggcgtcgcg ggcacgtgtc
      361 ctgtgcccgg agagggagat cgatggcgtt gcgtctggtc aggaacgtcg ggccgcgggc
      421 cggtgagagc ggccccggga aggacgcgac cgcggcgttc ctcctgatca acgggcgggg
      481 cggcgtcgtc gagtcggccg gctgggaggc gctgtcgctc gaaccggtgc cacagtcgat
      541 cccggcggcg gacgacgccc gcgagacgga ggacccgatc ctgcgcggca tcgccggagc
      601 ggtcgccgac gcacggcgcg gacgccgacc ttcgatccgg ctggtggaag tgaccctcca
      661 gcgcgcgcac gttttcgtga tcgtcgcggc tccgcttccc ggcgcgggac gcaacgagcg
      721 cgtcgcggtg accgtcgccg agaccggccc gcccgaggcc ggcggcaacg aggggaaagt
      781 catccgacag ctgggccacg acctgcgcac ccccctgacc tcgatcagcg gcgccatcga
      841 gctcctgcag tccggccggc tgggcgggct tcaggaaggc cagcagaagg tgctggggct
      901 cctggagaag ggggccgatg cgctcctgaa gctcatcaac gaggcgacct ttccctatcg
      961 cggtagccgc gacctggcgt cggtcctcgg cgtcggagtc gaggagctgg tggcgggggg
     1021 gctcgaagag ggggccgggc aggacgcgga cccgctcgag cccggcgacg acggtgacga
     1081 aacacccggc ggcgcggcgc cgtccggagc cggcgcccgg cggaaccgtc cgcggcggga
     1141 gcgaggacga tgaagcgcac gatcctggtc gcggacgact cgcaggcggt gcgcgacatc
     1201 ctccagatga gcctggagac gctcggctac accgtcgtcc tggcggagga cggtgagcgg
     1261 gcgatggcga ccatccaggc ctcccatccc gacctgatca tcgccgacgt catgatgccg
     1321 aaggtgaacg gcttccagat ctgccggcgg gtgaagtcgg atccctcgac ccgccgcacc
     1381 ccggtgatcc tgctcacggc ccgctccggc caggaggaca tcttctgggg gaaggactgc
     1441 ggcgccgacg actacatcac caagccgttc aagacgcagg acctcgagca gaccatccgc
     1501 cgcctgatgc gcgggcgcga tccgaacggc accggcccga tcgacatgga ccagcagcgc
     1561 cgccggcgcg tcgccgaggg ggggcagatc gtcatgctcc ggtgggatcc gcgcgccatg
     1621 gacgtcttcc ggaagaaata tggcgagatc gccttcagcc aggcgctcga tcggctgaag
     1681 gaggaggcgg aggcctacct cgccgagcgc agcgagccgg ggcccgtggg ggtgcacgtg
     1741 ccggtgggtt tgtcagtcgt cgtggccggc gtggcctcgc aggccatcgt cgtgggccag
     1801 gagctggcgg tgcgcctgag ccgcacggcc ctcgacctgt acgacggcga cgaccgggtg
     1861 cagggatgca tccgcttccg cgatccgcgg agcggacgcg aggagcgcct gccgctcctc
     1921 tccttctcgc cgcgcatcga tcccgacacg cttgtctaag agcgagggcc gatcggtgat
     1981 cccgaggaag ctgggccgct tcgagatcgt ccgggagatc ggacgcggcg ccatggggca
     2041 ggtcctcctg gcgcacgacc cgaagatcga tcgccaggtc gccctcaaga ccctcgcccc
     2101 gtcccccggg atctccgagg aggaggcgcg cgagacgagc cagcgcttcc tgcgcgaggc
     2161 gcaggcggcc ggcaagctcc tgcaccctca catcgtcacg atcttcgacg tcggcgagga
     2221 cgccggcctg tcgttcatcg ccatggagta catcgacggg gtgacgctcg agcgccacgc
     2281 ccgcgccgat tcgctcctgc cgccggcgcg cctgctcgag ctgatgacgc aggcgggggg
     2341 agcgctcgac tacgcccacc aggcgaaggt gatccaccgc gacatcaagc cggcgaacct
     2401 gatggtgcag gacggaaacc tcaaggtcac cgacttcggc ctggcgaaga acccgagcgc
     2461 cagcctcacg cagacgggcg tcctgctcgg cacgccttcg tacatgtccc ccgagcagat
     2521 ccaggggaag gacctcgacg ggcgcagcga tctgttctcg ctcggcgtcg tactctacga
     2581 gttgctgacc ggcgtgcggc cgttcgaggc cgattcgatc tcgaccatca tctaccgggt
     2641 cctctacgag gatccgcgcc cggccgcggc gcacaacccg gccctgccgc cggcggtgaa
     2701 cgccgtgctc gagaaggcgc tggcgaagga cccggcgcgc cgctacgcca ccggcgcggc
     2761 gctcgtcgcc gacctgcgca aggcgttcag cgatctcccg gccgagacgc tggcgcggcc
     2821 gttcccgatt ccgtccccgc ccccgggtgg acggcgccct tccgtcccgt cgatggcgac
     2881 ccggcccggg catggtccga tgcggccggc gcgtccgggc ggagcgaccg cgcgcacggc
     2941 gcgtccgccg cagccggccc cggcaccgca gccgaccttg ctcgctcacc atccggtcaa
     3001 gatcctcgca ctcgggctga cggtggtcct ggggatcgcg cttttcccgc gctgggccaa
     3061 ccggtcggag cggcgctccg cgcgccagag cgcaggcgcc gtccagctcc cggaggccgg
     3121 cccggcgcag gcggccggcg tcggcggtgt tcccgccgcg gcggcggcgt ccggcggccg
     3181 catcagcgtc gacgtcgaaa caaagccggc gggggggagc gtcttcctgg acgacatccc
     3241 gctcgccgag cccaaggtcg tcctggccct gtcggatccc acgacgcacg ccatccgcgc
     3301 ccgctccggc tgcctcgagg ggacggcgga gaaaagcgcc gccgacctcg cggcgcttca
     3361 cgggccgctc gtgctcgagc tcagggagcg acgcgaggtc gtggcgatcg gctcggagcc
     3421 gcccggcgcg cgcatccgca tcgacgggcg cgacaccggc aagacgaccc cggtcgagat
     3481 cgagatggac gcctgccgcg agcggaccgt cgatctgcgc ctcgacggcc acaggcggtg
     3541 gacgcggacc ttcaccgcgg gggaagatgt ggaggcggtg gcgggggcgc tctccgcggt
     3601 cgcgctcgag ccgatcccgc ggggcaccgt ggcgctcccg cggccgaagg gatggagcgg
     3661 cgacgtcttc ctcgggaacg cgcgggtcgg cggggcgggg gagccgatcg cgctcgacga
     3721 agggacttac acgctggtgg tgcgcaacga caaggtgttc gcccggacct cggtcccggt
     3781 gacggtgcgc gcgaacgaga cgacccgtcc cgcggtcgcc ctgccggggc tcggcagcct
     3841 caccgtgcag gcgcagccga gcaactgcaa ggtgtacgtg gacggcacct acgtggacgt
     3901 gacgccggtc ctcgacctgc cgattgccgc cggggcgcac gacatcaagg tggtgttcgt
     3961 gccgaccggc gcggcgaaga gcaagaac
//
LOCUS       DASRIQ010000115         9151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_304687, whole genome shotgun sequence.
ACCESSION   DASRIQ010000115 DASRIQ010000000
VERSION     DASRIQ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304687"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VFD06_02710"
     CDS             <1..1174
                     /locus_tag="VFD06_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02478.1"
                     /translation="SVLLVKPELTLRAPDSVPWSETWDVAVGPAWHLETEGPAPVHAE
                     EVPALRVRTWRPWPDEELRLVVTRPGAVDGRTLTIDRTDLTITPGLRATDAVLEMSAR
                     ASRGGEQALTLPDGAVLSGLTIQDRSQPFRQEGREVTVPIVPGAQNIRLEWREPRGLS
                     ASFRAAGVDAGIETTNATTVFAIPSDRWILFLGGPRLGPAVLFWSLLAISLIAALLLG
                     AIRMTPLRWQHWLGLSFGLTQVPIAASLVIAGWLLALGARRRRPPRNKWWFDLGQVAL
                     AFWTLVAMGLLFWAIQHGLLGDPEMQISGNGSSADRLVWYQDRTAGALPRPWVFSLPI
                     LYYRLAMLAWALWLAAALIRWLRWGWDSFSEGGVWKPLRTPKPESPSPPPLPVPSG"
     gene            1331..3025
                     /locus_tag="VFD06_02715"
     CDS             1331..3025
                     /locus_tag="VFD06_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN02479.1"
                     /translation="MTRRVSWWLLLASLLAFMPEKASAQPQVVAVDCNSSGMVSDHGG
                     WQAPGADPVSGDLACEPDGAFIFFANYQAYYEWLLLAPSFGDLTVETEVLFYEREGAD
                     TDAFSLLLRWNGSGSPACCEPAHAASGLRLLFSLARNRVEAFQEIDGASGPLLGTLPF
                     TLPPEMPRALKVNLRGTMLDLAVDGQPIGSLTVPDTPPALFGFDARGVVTRFAPFSLT
                     LRCQGDFDCDGVADVNDNCPSAPPADQTDTDGDGLGNPCDSDDDGDGVGDASDHCPLA
                     FDPGQEDADNDLVGDACDTCPLDAANDYDQDGFCTPADNCPFEANPDQADMNGNGVGD
                     FCDMNDGFLLIRWADKNRLTWQPEAPYNVFNLYRRDVAALRSTGVYVLDPYTGPGVGQ
                     RLCNINGDYTTTTVPALGQATAYFISGSMNGIDAGFGEDSSGNTRPNDYPCGCRPMVR
                     VLHNVDGYSLPANRIIDNLTDWCAFFPSACASGSVDFSTEVAVLHRWDGPCTSYDTEI
                     TCVEKAGTGVRVSGRNIEYQCPGCFFVIVAPYDVVKIARPATSATYSFGLTFPPCP"
     gene            complement(3027..3983)
                     /locus_tag="VFD06_02720"
     CDS             complement(3027..3983)
                     /locus_tag="VFD06_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HZN02480.1"
                     /translation="MQHWLSDIEAAAERIRPYTRLTPVDPSPELSRLGGSEVWLKMEH
                     LQVTGSFKLRGAMNRLLMLSPEERGRGVVAASSGNHGMAVSWGTRALGGKATIFCPEN
                     ASPVKLQAMRDHGAKVRARGTDSGLSEIEARRYAEEQRLAYVSPYNDSGVVAGQGTVG
                     LEIGGQVDRPDAVFVALGGGGLLSGIGLALEGMGRKATIVACSPANSAVMHHSLAAGR
                     IVEMESFDTLSDGTAGAVEAGAITFDLCRRLAADSVVVSEEEIRAAMRLVVERHHTLV
                     EGAAAVAVAGYLKMKDRFRGRRVVIVLCGANISLERLRAAIA"
     gene            4120..4443
                     /locus_tag="VFD06_02725"
     CDS             4120..4443
                     /locus_tag="VFD06_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02481.1"
                     /translation="MGGLGLAATLLTSPAAAGEIFGRVLQGNAPAAEGTTVAAKCGDA
                     DYAARPVDRSGNYHIVAGKTGKCTLTVTAKGQSATVDVVSYDDPVEADIVLDTKDGKL
                     SARRR"
     gene            4443..5315
                     /locus_tag="VFD06_02730"
     CDS             4443..5315
                     /locus_tag="VFD06_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02482.1"
                     /translation="MGEQKDLWDKFGVLLQPVGGALAALAVAVVGIRGSQVINERQAA
                     DTNARLYSELMSRREEAESSLRKDMFVSIIDSFLRPQPGDLGSMVLNLELLAYNFHES
                     LNLKPLFLDLQRRLRETSQANREEYRQRIDRVAREITARQLFSLEGHGDSFRRSVDLE
                     DLERTGRFGQELDAEEVQVDGTRVVVRVRALELDRANQQLIIRLEVSPAEGGPELTES
                     RATFQVGFYDFPLIDNTRLANGMRCAVTLSAWSKSNADLTTTCFPGEYASLKDRPYID
                     EVMKKLTKQAAPQP"
     gene            5482..8898
                     /locus_tag="VFD06_02735"
     CDS             5482..8898
                     /locus_tag="VFD06_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN02483.1"
                     /translation="MSRLRVIIPSAAALAAWFAFALLPAAGPGTVGAAEDTPPDGFYR
                     YPTVAKGMVVFASEGDLWKVPLAGGTAQRMTAYEGEEKFPALSPDGRFIAFTAQYEGN
                     DDVYVMSASGGEPARLTWHPATDQVIGWTPDGTILFRSRRDTPHGDYRIYTLPREGGI
                     PTLVKLEPAAWLSFEPKGKRVAFQKIGLETHNWKRYKGGEAEQIYVGTLEPLAMKETT
                     DWDGKDAFPMWAGDGRIYFVSDRWGRPNLASMKPDGTDVKRHTTFDDYDVRWPSLGDT
                     TIVFQHKMDLFSYDLASGKAAQIPVTLPSDRIQVRDRFVDPMANLRGWGLSKDGERIV
                     LETRGDLFVTRTKRKGLIRRLTENSAARTRGPAFSPDGTTVAGWTEIDGEEQLILDSA
                     DNSAAFRQVGKQEPGWHYAPVWSPDGKRLAWSDQKYRVMIADVAGGATTVVDTGQFET
                     DTYEWSPDSRYLAYEIPIPNGFSQVRIYDSQGKKSYDVSTPMFNSYSPTWDPKGKYLY
                     YLSDRWINPYLDRFEARFIVMKSALPIVVALQADGKLPFAPRSDMDPKDEKKDADKKD
                     ADKKSDDKSGDKKDADKKDDDKDKKKEDEKVEPIRIDFDGLADRIVQVPVPPGRYSGL
                     KAVEGKLHWLAFEARGMTPMEGPDDDDDDGPGGTLYTYDLAKEKMMPLAQGVRGYDVS
                     MNGKVLVYRTKDHFIRAEAGSMTTPQLDGPQGGADDPRVDLSGWTVRVNPRQEWKQML
                     REAWRLQRDFFYDPKMHGVDWEAVWKQYGELADRMASRDDLSDLLGEMFGELSVGHAY
                     HQPGDIRRGRPIGTGLLGADLAYDPSSGFWQIGKIYEADYPDPKTSSPLARPDFKVKP
                     GMWLVAIDGRALQKGEDYLRRLANRAGQEVELSVNDAPKEEGARRIIVKPVANDTRLR
                     YADWIRETRAYVDKKSNGQIGYLHLYDMGGFGLQQFARDYPPQWDKKGFIIDDRWNHG
                     GFVAPMIVAHLDRKLFSVAGLRYSSLRQTTPDRAFHGHLDVLINRQGGSDCETLALAV
                     KDFGLGQVIGTRTWGGWVGIRGDKPFRDGGVTTQPEFGGWDPKGKGWQIEGHGVDPDV
                     VIDLSPDGFLDGVDEQIDYAIKDLMDKIAKDPRDLAPMPPVPPRPLKTNR"
BASE COUNT         1490 a         3176 c         3122 g         1363 t
ORIGIN      
        1 ctccgtcctc ctggtcaaac cggagttgac gctgcgggct ccggactcgg tcccatggag
       61 cgagacctgg gacgtcgccg tcggccccgc ctggcacctc gaaacggagg ggccggctcc
      121 cgtccacgcc gaagaggtcc cggccctgcg cgtgcgcacc tggcggccgt ggccggacga
      181 ggagttgcgg ctcgtggtga cgcggcccgg agcggtcgac gggaggacgc tcacgattga
      241 ccggaccgac ctcacgatca cccccggcct gcgcgccacg gacgcggtgc tcgagatgtc
      301 ggcgcgcgcc agccgcggcg gcgagcaggc gctgacgctc ccggacgggg cggtgctgag
      361 cggcctcacc atccaggatc gatcgcagcc gttccggcag gaggggcggg aggtgacggt
      421 gccgatcgtc ccgggcgcgc agaacatccg gctggagtgg cgcgagccgc gcggcctgtc
      481 cgcctcgttc agggcggccg gagtcgatgc ggggatcgag acgacgaacg ccacgacggt
      541 cttcgccatc ccgtccgacc gctggatcct gttcctgggg ggcccccgcc ttggtccggc
      601 ggtcctcttc tggagcctgc tcgccatctc gctcatcgcg gccctgctcc tcggcgcgat
      661 ccggatgacc cccctgcgct ggcagcactg gctcggcctc tcgttcgggc tgacgcaggt
      721 cccgatcgcc gcgtcgctcg tcatcgccgg atggcttttg gcgctcggcg cccgccggcg
      781 ccggccgccg cggaacaagt ggtggttcga tctcggccag gtcgccctgg cgttctggac
      841 gcttgtcgca atggggctcc tgttctgggc gatccagcac gggctcctcg gcgatcccga
      901 gatgcagatc tccgggaacg gatcgtccgc cgatcgactc gtctggtacc aggaccggac
      961 cgccggggcc ctgccccggc cgtgggtctt ttcgctcccc atcctctatt accggctcgc
     1021 gatgctggcg tgggcgctgt ggctggcggc ggcgctcatc cgctggctgc gctgggggtg
     1081 ggactcgttc tccgagggag gcgtctggaa gccgctgcgg acgccgaagc cggagtcgcc
     1141 gagtccgccg cctctcccgg ttccgagcgg ctgagggccc ggactcccgt cggcggccgg
     1201 ccatccgcgg agtttggacc gcggccacac ccgcgttcct gccttgacaa tcgcgactcc
     1261 tgagcgtaga aaaatggcaa gctcgctttc tcgctcctcg ccgcggacag taagggttcc
     1321 ggaggcactc atgactcgac gagtgtcgtg gtggctgctc ctcgcttcgc ttctcgcctt
     1381 catgccggag aaggcttcgg cgcaacctca ggtcgtcgcc gtcgactgca acagcagcgg
     1441 catggtctcg gatcacggcg gctggcaggc gccgggggcc gacccggtga gcggcgacct
     1501 ggcatgcgag ccggacggcg cgttcatctt ctttgcgaac taccaggcct attacgagtg
     1561 gctgctgctc gccccgagct tcggggacct cacggtcgag acggaggtgc tgttctacga
     1621 gcgtgaaggc gccgacacgg acgccttcag cctgctcctg cgctggaacg gatcgggcag
     1681 cccggcctgc tgcgagccgg cgcacgctgc gtccgggctg cgtctcctgt tcagcctcgc
     1741 gcgcaatcgc gtggaggcgt ttcaggagat cgatggagcg agcgggcccc tcctggggac
     1801 gctgcctttc acgctgccgc ccgagatgcc gcgggcgctc aaggtgaacc tgcgcggcac
     1861 catgctggat ctggccgtgg acggacagcc catcggttcg ctcaccgtcc ccgacacgcc
     1921 gccggcgctt ttcggattcg atgcgcgcgg tgtcgtcacc cgtttcgctc cattttccct
     1981 gaccctccgg tgtcagggag atttcgactg cgacggcgtg gccgatgtga acgacaactg
     2041 cccctcggcg ccgccggccg accagaccga caccgacggc gacgggctgg ggaatccgtg
     2101 cgattcggac gacgacggcg acggcgtcgg cgacgcttca gaccattgcc cgctcgcctt
     2161 cgatcctggg caggaagatg ccgacaacga tctggtcggc gacgcctgcg acacctgtcc
     2221 gctcgacgcc gcgaacgact acgaccagga cgggttctgc acgccggccg acaactgtcc
     2281 tttcgaggcc aacccggatc aggcggacat gaacgggaac ggcgtggggg atttctgcga
     2341 catgaacgac gggttcctgc tcatccgctg ggccgacaag aatcggctga cctggcagcc
     2401 cgaggccccc tacaacgtgt tcaacctcta caggagggac gtggcggctc tccggtccac
     2461 aggcgtgtac gtgctcgacc cgtacactgg ccccggcgtg ggtcaacgcc tgtgcaacat
     2521 caatggggac tacacaacga ccaccgtgcc ggcccttggg caggcgaccg cctacttcat
     2581 cagcggtagc atgaacggaa tcgacgccgg gttcggggag gacagcagcg gcaacacgcg
     2641 tccgaacgac tatccctgcg gctgccgtcc gatggtgcgc gtgctgcaca atgtcgatgg
     2701 ctactccctg ccggcgaacc ggatcatcga caatctcacg gactggtgcg cctttttccc
     2761 ttccgcctgc gcctcgggat cggtcgattt cagcacggag gtggcggtgc tccacaggtg
     2821 ggatggaccc tgtacctcct atgacacgga gattacctgc gtggagaagg caggcaccgg
     2881 cgtgcgcgtg agcggccgga acatcgaata ccagtgtccg ggatgcttct ttgtgatcgt
     2941 ggcgccctac gacgtcgtga agatcgcccg tccggcgacc tcggcgacct actcctttgg
     3001 attgaccttt cccccttgtc catgactcag gcgatcgccg cgcgcaggcg ctcgagcgag
     3061 atgttggcgc cgcacaggac gatgacgacg cggcggccgc ggaagcggtc cttcatcttg
     3121 agataaccgg ccaccgcaac cgccgccgcc ccctcgacga gcgtgtggtg gcgctcgacg
     3181 acgagacgca tcgcggcgcg gatctcctct tcgctcacga cgaccgagtc ggcggcgagg
     3241 cgccggcaca ggtcgaaggt gatcgccccc gcctcgaccg cgccggcggt gccgtccgac
     3301 agggtgtcga acgactccat ctcgacgatg cggccggcgg cgagagagtg gtgcatcacc
     3361 gccgagttgg ccggcgagca ggcgacgatc gtcgccttgc gtcccatccc ttcgagcgcc
     3421 aggccgatgc cggagaggag cccgccgccg ccgagcgcga cgaagaccgc gtcgggccgg
     3481 tccacctgcc cgccgatttc gaggccgacc gtcccctgcc ccgcgaccac gccggagtcg
     3541 ttgtacgggg agacgtaggc gagccgctgc tcctccgcgt agcgccgcgc ctcgatctcg
     3601 gacaggccgc tgtcggtgcc gcgggcgcgc accttcgcgc cgtggtcgcg catcgcctgc
     3661 agcttgacgg gggaggcgtt ctccgggcag aagatggtcg ccttgccgcc gagggcgcgc
     3721 gtcccccacg acaccgccat gccgtggttc cccgacgagg cggcgacgac gccccggccc
     3781 cgctcctccg ggctgagcat gagcagccgg ttcatcgcgc cgcgcagctt gaaggagccg
     3841 gtgacctgga ggtgctccat cttcaaccac acctccgatc cgccgaggcg cgacagctcg
     3901 ggggacggat cgaccggcgt caggcgagtg tacggacgga tccgttccgc cgccgcttcg
     3961 atgtccgaaa gccagtgctg catgaacggc catgatagcc cggcgcgttc gcggcgccgc
     4021 ccccggccgg cgggttggcg gagacgcgcg cttgcggtaa tctccgcgcg tccctcggga
     4081 gcccacatga cacgatcacg cacgcggaca ttcacagcgg tggggggcct cggcctcgcc
     4141 gcgacacttc tcacctctcc cgccgcggcc ggggagatct tcgggagggt gctgcagggg
     4201 aacgccccgg ccgcggaggg aacgacggtc gccgcgaagt gcggcgacgc cgactacgcg
     4261 gcgaggccgg tcgaccgctc cggcaactat cacatcgtcg ccggcaagac cggcaagtgc
     4321 accctgacgg tgaccgccaa gggccagtcg gcgacggtgg acgtcgtttc ctacgacgac
     4381 ccggtggagg ccgacatcgt cctcgacacg aaggacggca agctcagcgc ccggcgtcgc
     4441 tgatggggga gcagaaggat ctctgggaca agttcggcgt cctgctgcag ccggtcggcg
     4501 gggcgctcgc ggcgctcgcc gtggcggtcg tcgggatccg cggctcgcag gtgatcaacg
     4561 agcgccaggc ggcggacacc aacgcccgcc tctactccga gttgatgagc cgccgcgagg
     4621 aggcggagtc gagcctgcgc aaggacatgt tcgtgtcgat catcgactcg ttcctgcggc
     4681 cgcagccggg cgacctcggg tcgatggtgc tcaacctcga gctgctcgcc tacaacttcc
     4741 acgaatcgct caacctcaag ccgctttttc tcgatttgca gcggcgcctc cgcgagacgt
     4801 cgcaggccaa ccgcgaggag taccgccagc ggatcgaccg cgtggcgcgc gagatcaccg
     4861 cgcggcagct cttctcgctc gaagggcacg gcgattcctt ccggcgctcg gtggacctcg
     4921 aggacttgga gcgcaccgga cgcttcgggc aggagctcga cgccgaagag gtgcaggtgg
     4981 acggcacgcg cgtggtggtc cgcgtgcggg cgctcgagct ggatcgagcg aaccagcagc
     5041 tcatcatccg cctcgaggtc agccccgcgg aaggggggcc ggagctgacc gagtcgcgcg
     5101 ccaccttcca ggtcggcttc tacgatttcc ccctcatcga caacacccgt ctcgccaacg
     5161 gaatgcggtg cgccgtgacc ttgagcgcct ggtcgaagtc gaacgccgac ctcaccacca
     5221 cctgcttccc cggcgagtac gccagcctca aggaccgccc ctacatcgac gaggtcatga
     5281 agaagctgac caagcaggcc gcgccgcagc cctgaccctc gcgtgcgccc gcgccgcggg
     5341 caggagcccg gcgacgcccg gaaccttccc ctcccgcctc gcgtagatga gcccgccagc
     5401 gcccgggccg ggagtgctcc gggcgctcat gtagaatcgc gccttccgtc aacgatcctt
     5461 acaggacctg gggggagacc catgtcacgt ctgcgcgtca tcattccgtc cgccgccgct
     5521 ctcgcggcct ggttcgcgtt cgccctgctt ccggccgccg gccccggcac ggtcggcgcg
     5581 gccgaggaca cgcccccgga cggcttctac cgctacccca cggtcgccaa ggggatggtc
     5641 gtcttcgcct cggaggggga tctctggaag gtgccgctcg cgggcggcac ggcgcagcgg
     5701 atgacggcgt acgaggggga ggagaaattc ccggccctct cccccgacgg gcgcttcatc
     5761 gcgttcacgg cgcagtacga agggaacgac gacgtctacg tcatgtccgc ctcggggggg
     5821 gagccggcgc ggctcacctg gcacccggcg acggatcagg tgatcggctg gacgccggac
     5881 gggacgatcc tcttccggag ccgccgcgac acgccgcacg gcgactaccg catttacacc
     5941 ctgccgcgcg agggagggat cccgaccctc gtcaagcttg agccggccgc ctggctctcc
     6001 ttcgagccga aggggaagcg cgtggcgttc cagaagatcg gcctcgagac ccacaactgg
     6061 aagcgctaca aggggggcga ggcggagcag atctacgtcg gcaccctcga gccgctggcg
     6121 atgaaggaga cgaccgactg ggacgggaag gacgccttcc cgatgtgggc gggcgacggc
     6181 cgcatctact tcgtcagcga tcgctggggg cgcccgaacc tcgcgtcgat gaagccggac
     6241 ggcaccgacg tcaagcgcca caccacgttc gacgactacg acgtccgctg gccgtcgctc
     6301 ggcgacacca ccatcgtctt ccagcacaag atggatctct tttcgtacga cctcgcgtcg
     6361 gggaaggcgg cgcagatccc ggtcaccctg ccgagcgacc gcatccaggt ccgggaccgt
     6421 ttcgtcgacc cgatggcgaa cctgcgcggc tgggggctgt cgaaggacgg cgagcggatc
     6481 gtcctcgaga cgcgcggcga tctcttcgtg acgcgcacga agcgcaaggg gctcatccgc
     6541 cgcctcaccg agaactcggc cgcccggacg cgcggcccgg ccttctcgcc cgacggcacc
     6601 acggtcgcgg gctggaccga gatcgacggc gaggagcagc tcatcctcga ctccgccgac
     6661 aacagcgccg ccttccggca ggtcggaaag caggagccgg gctggcacta cgcgccggtg
     6721 tggtcccccg acggcaagcg cctcgcgtgg agcgaccaga agtaccgcgt gatgatcgcc
     6781 gacgtggcgg gtggcgccac caccgtcgtg gacaccgggc agttcgagac cgacacgtac
     6841 gagtggtccc ccgacagccg gtacctcgcc tacgagatcc cgatcccgaa cggattcagc
     6901 caggtgcgca tctacgacag ccaggggaag aagtcgtacg acgtctcgac accgatgttc
     6961 aactcgtact cccccacctg ggatccgaag gggaagtacc tctactacct ctccgatcgc
     7021 tggatcaacc cgtacctcga ccggttcgag gcccgcttca tcgtcatgaa gtccgcgctg
     7081 ccgatcgtcg tcgccctgca ggcggacggc aagctgccgt tcgcgccgcg cagcgacatg
     7141 gatccgaagg acgagaagaa agacgccgac aagaaagacg cggacaagaa atccgacgac
     7201 aagagcggcg acaagaagga cgccgacaag aaagacgacg acaaggacaa gaagaaggaa
     7261 gatgaaaagg tcgagccgat ccggatcgac ttcgacgggc tcgccgatcg catcgtgcag
     7321 gtgccggtcc cgcccggccg ctactccggc ttgaaagcgg tggagggaaa gctccattgg
     7381 ctcgccttcg aggcgcgggg catgacgccg atggagggac cggacgacga cgatgacgac
     7441 ggccccggag gcaccctcta cacctacgac ctcgccaagg aaaagatgat gccgctggcg
     7501 cagggggtgc gcggctacga cgtgtcgatg aacggcaagg tcctcgtcta ccggacgaag
     7561 gatcacttca tccgggcgga ggccggctcg atgacgacgc cgcagctcga cggcccgcag
     7621 gggggcgcgg acgacccgcg cgtcgatctc tccggctgga cggtgcgcgt caacccgcgc
     7681 caggagtgga agcagatgct gcgcgaggcc tggcgcctgc agcgcgactt cttctacgac
     7741 ccgaagatgc acggcgtgga ctgggaggcg gtctggaagc agtatggcga actggcggac
     7801 cgcatggcga gccgcgacga tctcagcgat ctcctcggcg agatgttcgg ggagctcagc
     7861 gtgggccacg cctaccacca gccgggggac atccgccgcg gccggccgat cgggaccggg
     7921 ctcctcggcg ccgacctggc ctacgacccg tcctcgggct tctggcagat cggcaagatc
     7981 tacgaggccg actacccgga tccgaagacc tcctcgccgc tggcgcgccc cgatttcaag
     8041 gtcaagccgg ggatgtggct cgtggccatc gacggccgcg ccctgcagaa gggggaggac
     8101 tacctccggc gcctcgccaa ccgggccgga caggaagtgg aactgtcggt gaacgacgct
     8161 cccaaggaag aaggggcgcg ccgcatcatc gtcaagccgg tggcgaacga cacccgcctg
     8221 cgctacgccg actggatccg cgagacccgc gcgtacgtgg acaagaagag caacggccag
     8281 atcggctacc tgcacctcta cgacatgggg ggcttcggcc tgcagcagtt cgcgcgcgac
     8341 taccccccgc agtgggacaa gaaaggcttc atcatcgacg accgctggaa ccacggcggc
     8401 ttcgtcgccc cgatgatcgt cgcgcacctc gaccgcaaac tcttctccgt ggccgggctg
     8461 cgctacagca gtctccggca gaccacgccg gatcgggcct tccacggcca cctcgacgtc
     8521 ctgatcaacc ggcagggtgg ctcggactgc gagaccctgg ccttggcggt caaggacttc
     8581 ggtctcgggc aggtgatcgg cacacggacc tgggggggct gggtcggcat ccgcggcgac
     8641 aagccgttcc gcgacggcgg cgtcacgacg cagccggagt tcggcggctg ggacccgaag
     8701 gggaagggct ggcagatcga ggggcacggc gtggacccgg atgtcgtcat cgacctcagc
     8761 ccggacggct tcctcgacgg ggtggacgag cagatcgact acgcgatcaa ggacctgatg
     8821 gacaagatcg ccaaggatcc gcgcgatctc gccccgatgc cgccggttcc accgcggccg
     8881 ctcaagacca acaggtgaaa cgtcgggccg ggagcgggcc gcatcgtggc ccgctcatca
     8941 ggtgctggcg cgccccgaga cccggcatta cattgtggta ggatgcgtgc ctcgaggcgc
     9001 tgtcgcgcgc gccccgaggt ggcgccccag gggggcgtca ccctcaggca caacaccttt
     9061 caggagaaca cgttgagaga agcgacccag cgggaaccgt tcatcctgtc cgtcgtgcgg
     9121 tggttcgatt cctggcaggg gatccaccac g
//
LOCUS       DASRIQ010000116        13446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1608110, whole genome shotgun sequence.
ACCESSION   DASRIQ010000116 DASRIQ010000000
VERSION     DASRIQ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 13446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13446
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608110"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFD06_02740"
     CDS             complement(<1..521)
                     /locus_tag="VFD06_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZN02484.1"
                     /translation="MKGVILAGGLGTRLFPLTKITNKHLLPVYDRPMIHYPLKTLSDA
                     GIRDVMIVTGGNHSGEFLRLIGNGKEYGLNEVAYAYQEGEGGIADALALARPFVKGDR
                     MVVILGDNIIEGDIRRPVAAFAEQKKGARILLKKVSDPERFGVPIFDGGKITGIEEKP
                     KTPQSEYAVIGIY"
     gene            complement(607..1887)
                     /gene="aceA"
                     /locus_tag="VFD06_02745"
     CDS             complement(607..1887)
                     /gene="aceA"
                     /locus_tag="VFD06_02745"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZN02485.1"
                     /translation="MSVVAPASGSAVKKPGQASERFAGIVRPYATKDVERLRGSVRIE
                     HTLARLGAERLWSLMTEGPYVHALGAVTGNQAMQMVRAGLKAIYCSGWQVAADANLQG
                     QTYPDQSLYPANSVPALVKRLNQALQRADQIEHAEGGTKRHWFAPIVADAEAGFGGPL
                     NAFELMKAMIESGAAGVHYEDQLSSEKKCGHLGGKVLVPGAHFVRTLVAARLAADVLD
                     VPTVLIARTDADSARLLTSDVDPRDREFLTGERTPEGFHRITGGLEMAIARGRAYAPY
                     ADLLWCETSTPDLDEARRFAEGIHQEFPGKMLAYNCSPSFNWKKHLSSSDIARFQREL
                     GAMGYRFQFVTLAGFHALNHGMFDLAMQYRDRGMAAYSDLQEKEFAAETSGYSATRHQ
                     REVGTGYFDEVARIISDGGASTLALDDSTEKAQF"
     gene            complement(1909..3504)
                     /gene="aceB"
                     /locus_tag="VFD06_02750"
     CDS             complement(1909..3504)
                     /gene="aceB"
                     /locus_tag="VFD06_02750"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HZN02486.1"
                     /translation="MTTRPRPVSPAGLVVRGPQVQEAASVLTPAALAFVEDLARRFGP
                     EVARLLERRRARQAEWDRGARPRFLPETREIRDSEWTVAPLPGDLLDRRVEITGPVDR
                     KMILNALNSGASVFMADFEDATTPTWENLVRGQANLREAVAGTLRHTDPATGKEYRLG
                     AKTAVLCVRPRGWHLLEAHVLLDDAPIPGALFDFGLFFFHNARALVERGTGPYFYLPK
                     MQSHLEARLWNDVFLRAQEAIGLPAGTIKATVLIETLPAAFEMDEILWELRQHSAGLN
                     CGRWDYIFSFIKTRRADSAAVLPDRAQVTMEQPFMRAYTQHLVRVCHRRGVHAMGGMA
                     AQIPIKNDPAAGEAALAKVRADKEREAKDGHDGTWVAHPGLVPLAREVFDRFMPGPNQ
                     IARRRDDVVTREEDLLVPPQGTRTEAGLRRAVLVGVRYLDAWLSGLGCVPLDHLMEDA
                     ATAEICRTQIWQWVRHGARLEDGRVVDAPLVRRILAEETKGQRLHPDTVPLFEQVALS
                     DPLIDFLTLPAYERLTALEPASE"
     gene            complement(3600..4313)
                     /locus_tag="VFD06_02755"
     CDS             complement(3600..4313)
                     /locus_tag="VFD06_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02487.1"
                     /translation="MKAVRLLAGLLLGGLLVGVAWYAGWVMPPEPLARRVGVWATLYP
                     LSTKGAAVEPIPCEPLRRIRFYVVCTEGCDGVWRIFAMKGLRTIQLANLARIPPEGVT
                     ATRRRINDIVRAERVIVDDDQARLMVEFYLRLDGLATGRLLTEADRLAVEETRTEGEE
                     ALKALADTIAEGDALARIPIERGEAGIHSTLLYWDTSRRGWPVLEIAFRLGGFGEVRD
                     LRVRQLSTSEVEEEAESGE"
     gene            complement(4310..5947)
                     /locus_tag="VFD06_02760"
     CDS             complement(4310..5947)
                     /locus_tag="VFD06_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN02488.1"
                     /translation="MNRRRAVNAALLAFGLLLFGWRLGGHDLWPPDEPRFGLVAREML
                     EGGDPIVLSRNGGLYTDKPPLFFWAIDAAAILRGGEVDETAARMPSLVASMLALLLVA
                     RLGERLYDRRTGILGAVLFATNLQILERARWASIDMTLNLFVLLAFVLLWEEVEGAGS
                     RWHWRAVAAWGAMGLATLAKGPVGLVLPLLALVPALALLRRGRDLPRLFPAAGVILFL
                     AVVGGWFSLFASRLGWSEAVGIATHQTVDRYVNAWNSQHPVWYYLWRFPAGFLPWCMF
                     LPAALLAAWRPDKAGEPAASSRARERTATVFLTVWFAAIFLFFSFSTGKRGVYIIPLY
                     PAAVLLVARLFTRDGSARRAARAALAFAPLACAAALALVLAVPRHHPEFRLGAWTIAA
                     LLAASAACAAWGGARRRTWLVPWGIAAAMGLLMLFAAESVLPQVDRRFNLSGFAAEVK
                     ARRDPSVPLGATEEKRETWAFYLHTTVEELDHPDAILAWIGSGPDRDLLIEDESYRAI
                     RERLPPEVSVAYTSRVSSRPFHLLVRRAPAAAAEGPR"
     gene            complement(6407..7627)
                     /locus_tag="VFD06_02765"
     CDS             complement(6407..7627)
                     /locus_tag="VFD06_02765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN02489.1"
                     /translation="MRLLVVLNGGADHPSTRFRVLQHLDRLRARGIEPEPLVAKRTEG
                     YGPIQLRRHAARCDAVLIQKKLFSPWKLHLLPQGVPVAFDFDDALFEASPTEAARYGQ
                     EKAERRAAARRRRLQALLRRAALVLAGNRFLSEHAARLAPARAPRIEILPTGVDLRPF
                     PEERVRAAAARRAASTGGPRIGWIGSRASLRYLATLASPLRAACARVPGTRLVQVCND
                     FLDLPGVPTEKVIWSAEREEEDLLGFDVGLMPIDDRPFSHGKCGLKILQYQAAGVPVV
                     CSPVGANLEIVEPEVTGLFATGEAEWTQAMVRLLQDRERGRRFAAAGRARVEAVYSAD
                     RIGARLADALATLKTPAGRKRGRDAVVAGSGGDDAGRKPGERRNKPEEHERLLVADHL
                     RERPAVLAARVRPK"
     gene            7722..8678
                     /locus_tag="VFD06_02770"
     CDS             7722..8678
                     /locus_tag="VFD06_02770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN02490.1"
                     /translation="MQNEPELSVVIPTYGKAETLPLVMRALEGQSIGRERFEVVVIDD
                     GSPDDTTGRLRTLEGSTPLRFRWMTQENRGVSATRNRGAREARAPILLFIQDDIVARP
                     DLLERHLAIHARHPEATAAGVGRVTWPPDWSIDHFMAWLDNGGPQFRYHQVAGRATVT
                     FKHFYTCNVSLKRRALLDHPFDEEIVYGFEDLELALRLERAGFVFHYDEEALGYHHHR
                     RSFEDYRRRQFKAGQSLYVAFRNHPELVGRTGITSIRPSKRTRTRLRGAILPVARLVG
                     ARRTIEKYWRAVLDEELVAGYEQARDRDLRRGSAPRPAPAAS"
     gene            complement(8635..9570)
                     /locus_tag="VFD06_02775"
     CDS             complement(8635..9570)
                     /locus_tag="VFD06_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02491.1"
                     /translation="MRGIGLAIAFAAVAFTSDLFAADPTAGAAAAAEDPAAEDPVAAG
                     VAANDPLAADAPLDWRQIGRDARYVFGRPAHLDTAGWVRLGVGVGAGASLYLVRDEAR
                     DVALEHGGERWDDLLDDARLMGRLATPLLAAGGFYLAGLARDSDRDRETAIVLMESLA
                     FSSAIAATANYVVAAERPEDGDDVTFFDRDGHSVSLDVTIAASMLAPIIDRHLRVRPE
                     DRGSVRFWKRFGAWGLYGTAGLVALQRINEDAHWLPDVYFGYLNGLCVGRMVVDSRRG
                     GRAERDRAAGAPGEAVARPARVTMRPVPGGVRILF"
     gene            complement(9567..11240)
                     /locus_tag="VFD06_02780"
     CDS             complement(9567..11240)
                     /locus_tag="VFD06_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN02492.1"
                     /translation="MNDRPSGPRPAVLLFATDRRVLLVLLAASLLLVATGIGGRDLWD
                     PDEPRTAVITAGLLRDGRWAAPEQHGHPWLEKPPLYYWLASAASSALGGVSETSARIP
                     ANAAAVLTALVLFALGRSLFGRRCGGLAALVLLTTEDFLIEARWARPDMLLALLLTAA
                     VLCLWHATQAGGAGWWAGFFLACGLGVLAKGPVGLLPIPGALLYLASTRRLRRLLQPD
                     WIVSLPLLFVPAGLWMLAWSASTGERFPLGELLGRFAERVVSGLHHPRPAWHVLTTLP
                     LALLPWIALLPATIAETWPRPGRPRDDRLAFVYSLLASDLLIFAASVEKRGVYLLPMV
                     PLIALLIGRYWDVSLFDWDPPPARRLVSAGLWCWAAIVAAAAIVVWRRAGREAPDLVT
                     AGSWLGIAGLMAFLPPALLLRRIGPGRAIGVFAAGAAATGLVIVHLVLPALDPLKSAR
                     PLAARVAEATGEAPIGIHPDPHAGLAWYLARPVTLLPDREALFRFLDPPGRGVGLVER
                     TAWDLAQASAPDGTEVVLSGRVGHREFVLVRRTGDGAEVPRDAPADGAP"
     gene            complement(11237..13105)
                     /locus_tag="VFD06_02785"
     CDS             complement(11237..13105)
                     /locus_tag="VFD06_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN02493.1"
                     /translation="MKDLLRLVQYVKPYWRRVAAAIVSAVLISACWLALLMLVRPILD
                     EVLPKSAATATATAGKFDLLDKTRDMIDAGASRFPLLARWSQTLHDGSRGTAVLVAIL
                     IVVVFLFKGLFTYLTAYLTRWTGLQAVKDLRVDLYARIQRQSLAFFSEHSTGHLISRI
                     MGDVSRLQRTVSGDLAEVFRLAAIVLGQATYLFYLNWRLAGFCLVLLPIIVAPVARIG
                     RRLKEASRGSMQKMGDAALIMKEGLSGTRIVQAFGMEDFETRRFTRALDRMQRAEKKA
                     ARLQSITGPVMETLGSFGAAVLFVWAGHRIAADKLSAGEFGMFVTALFVIFASIRNLV
                     KINNDLQQAMAATRRVFEIMDQAPQVLEKPGASDLAPFSDRIEYRGVSFSYGRAPVLQ
                     ALDLIVNRGQVVAIVGSSGAGKSTLVNLLPRFYDVTAGSLLVDGVDVRDVSLASLRSQ
                     IGLVTQEVILFDETVRNNIAYGRSDIPQERVIEAARAAHAHPFIEALPQGYDTPLGES
                     GHRLSQGQRQRLSIARALLKNSPILILDEATSSLDSESENEVQAALENLMQGRTVFVI
                     AHRLSTVRRADAILVLDQGRIVERGAHAELLARSGVYARLHHLQFRDDTTPRASVR"
     gene            complement(13136..>13446)
                     /locus_tag="VFD06_02790"
     CDS             complement(13136..>13446)
                     /locus_tag="VFD06_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZN02494.1"
                     /translation="TRLDAAGASGFYHVHRERPFFQSLVSFMTSGPIVPMIVEGPEAI
                     ATVRRIMGATDPAKAEAGTIRKDLASNIEQNIVHGSDGKETAAFETAYFFSALEVPPR
                     "
BASE COUNT         2062 a         4806 c         4777 g         1801 t
ORIGIN      
        1 atatagatgc cgatgacggc gtactccgac tggggtgtct tcggcttctc ctcgataccg
       61 gtgatcttgc ccccgtcgaa gatgggcacg ccgaagcgct cggggtcgga gaccttcttg
      121 aggaggatgc gggccccctt cttctgctcc gcgaacgccg cgaccggacg ccggatgtcc
      181 ccctcgatga tgttgtcgcc gaggatgacc accatccggt cccccttgac gaacgggcgg
      241 gccagggcga gcgcgtcggc gatcccccct tccccctcct ggtaggcgta cgcgacctcg
      301 ttgaggccgt actccttgcc gttgccgatg agacgcagga actccccgga gtggttcccc
      361 cccgtgacga tcatcacgtc ccggatgccg gcgtccgaca gtgtcttcag cgggtagtga
      421 atcatcggcc ggtcgtacac cggcaggagg tgcttgttgg tgatcttcgt gagcgggaag
      481 aggcgcgtgc cgagccctcc cgccaggatg acgcccttca tggcggcttt ctccgtggga
      541 tgcgcgggac gttagcagag tcgccgggcg gtgtaaaggc gagccatcag gacgaccagg
      601 cggacgctag aactgggctt tttcggtcga gtcgtcgagc gccagcgtcg aggcgccgcc
      661 gtcggagatg atccgggcca cctcgtcgaa gtagccggtg ccgacctcgc gctggtggcg
      721 cgtggcgctg tagccggagg tctccgcggc gaactccttc tcctggaggt ccgaataggc
      781 cgccatgccg cggtcccggt actgcatcgc caggtcgaac atgccgtggt tgagggcgtg
      841 gaagccggcc agcgtgacga actggaacct gtagcccatg gccccgagct cccgctggaa
      901 gcgggcgatg tcggaagacg acaggtgctt cttccagttg aacgacggcg agcagttgta
      961 ggccagcatc ttcccgggga actcctgatg gatcccctcg gcgaagcggc gcgcttcgtc
     1021 gaggtcgggg gtcgaggtct cgcaccacag gagatcggcg tacggggcgt aggcgcggcc
     1081 gcgcgcgatc gccatctcca gcccgccggt gatgcggtgg aacccttccg gcgtccgctc
     1141 ccccgtcagg aactcccggt cgcgcgggtc cacgtcggag gtcaggaggc gggcgctgtc
     1201 ggcgtcggtg cgggcgatga gcaccgtcgg cacgtccagg acatcggcgg cgagccgggc
     1261 cgcgaccagc gtgcggacga aatgcgcgcc cgggaccagc accttgccgc cgaggtggcc
     1321 gcacttcttc tcgctcgaga gctggtcttc gtaatggacc ccggccgcgc cggattcgat
     1381 catcgccttc atcagctcga aggcgttgag cggcccgccg aagccggcct cggcgtcggc
     1441 gacgatcggc gcgaaccagt gccgcttcgt ccccccctcg gcgtgctcga tctggtcggc
     1501 ccgctgcagc gcctggttga ggcgcttcac cagcgcggga acgctgttgg ccggatagag
     1561 cgactggtcc gggtaggtct gcccctggag gttcgcgtcg gccgccacct gccagccgct
     1621 gcagtagatc gccttcagtc cggcccgcac catctgcatc gcctggttgc cggtgaccgc
     1681 gcccagggcg tgcacgtacg gtccttcggt catgaggctc cagagccgct cggcgccgag
     1741 acgggcgagc gtgtgctcga tgcggaccga tccgcgcagc cgctcgacgt ccttcgtcgc
     1801 gtacggccgg acgattccgg cgaagcgctc cgaagcctgg cccggcttct tcacggcgct
     1861 gcctgaggcg ggtgcgacga ccgacatgtt cggagctcct ttattccttc attcggacgc
     1921 cggctcgagc gccgtcaggc gctcgtaggc cggcagggtc agaaagtcga tgagcgggtc
     1981 ggagagggcc acctgctcga acaggggcac ggtgtccgga tgcaggcgct gtcccttcgt
     2041 ctcctcggcg aggatccgcc gcacgagcgg agcgtcgacg acgcgcccgt cctcgagccg
     2101 cgcgccgtgg cggacccact gccagatctg ggtgcggcag atctccgcgg tcgcggcgtc
     2161 ctccatgagg tggtcgagcg gcacgcagcc gaggccggag agccacgcgt cgaggtagcg
     2221 cactcccacg aggacggcgc gccgcagccc cgcctcggtg cgcgtcccct gcggggggac
     2281 gagcagatcc tcctcgcgcg tgacgacgtc gtcgcggcgg cgggcgatct ggttcggacc
     2341 cggcatgaac cggtcgaaca cctcgcgcgc cagcggcacg aggcccgggt gcgccaccca
     2401 ggtcccgtcg tggccgtcct tcgcctcccg ctccttgtcg gcccgcacct tcgcgagggc
     2461 cgcttcgccc gccgcgggat cgttcttgat cgggatctgc gccgccatcc cccccatggc
     2521 gtgcaccccg cgccggtggc aaacgcgcac caggtgctgg gtgtaggcgc gcatgaacgg
     2581 ctgctccatc gtcacctggg cgcgatccgg cagcaccgcg gcggagtcgg cgcggcgcgt
     2641 cttgatgaac gagaagatgt agtcccagcg gccgcagttc aggccggccg agtgctgacg
     2701 cagctcccag aggatctcgt ccatctcgaa cgcggcgggg agcgtctcga tcagcaccgt
     2761 cgccttgatc gtcccggccg gaagaccgat cgcctcctgg gcgcgcagga agacgtcgtt
     2821 ccagaggcgc gcctcgaggt ggctctgcat cttggggaga tagaagtacg gcccggtgcc
     2881 gcgctccacg agggcccggg cattgtggaa gaagaagagg ccgaagtcga acagcgcccc
     2941 ggggatcggc gcgtcgtcga gaaggacatg cgcctcgagc aagtgccagc cgcgcggccg
     3001 cacgcacagg acggcggtct tcgccccgag ccggtactcc ttgcccgtgg ccgggtcggt
     3061 gtggcgcagc gtgccggcca ccgcctcgcg aagattggcc tggccgcgca ccaggttctc
     3121 ccaggtcggc gtcgtcgcgt cctcgaagtc ggccatgaag acgctcgcgc cggaattgag
     3181 cgcgttgagg atcatcttgc gatcgaccgg gccggtgatc tcgacgcggc ggtcgaggag
     3241 atccccgggg agcggggcga cggtccactc cgagtcccgg atctcccggg tctccggcag
     3301 gaaccgcggg cgggcccccc gatcccattc cgcctgccgc gcgcggcggc gctcgagcag
     3361 ccgggccacc tcggggccga aacggcgcgc caggtcttcg acgaaggcga gggcggcggg
     3421 ggtcagaacc gaggccgctt cctgcacctg cgggccgcgc acgacgagcc cggccgggga
     3481 caccggccgc ggacgcgtcg tcatcgaacc ctcctcgggc ggatcgtcat cgcattgcct
     3541 cggcggaaag caccccggaa catgtcatga cctctccccc agggcaagcg acagtttcgt
     3601 cattccccgc tttccgcctc ctcctccacc tcgctggtgg agagctgccg cacccggaga
     3661 tcgcgcacct ccccgaaacc cccgaggcgg aaggcgatct ccagcaccgg ccacccccgc
     3721 cgcgaggtgt cccagtagag gagagtcgaa tggatccccg cctcgccccg ctcgatcggg
     3781 atgcgcgcga gcgcgtcccc ctcggcgatg gtgtcggcga gcgccttgag cgcctcctcc
     3841 ccctccgtcc gtgtctcctc caccgccagg cgatccgcct cggtcagcag gcgcccggtg
     3901 gcaagcccgt cgagccgcag atagaactcg accataaggc gcgcctggtc gtcgtccacg
     3961 atgacccgct cggcccggac gatgtcattg atgcgccggc gcgtggccgt gacgccctcg
     4021 ggcgggatgc gcgcgagatt cgcgagctgg atcgtccgga gccccttcat cgcgaagatg
     4081 cgccagaccc cgtcgcatcc ttcggtgcag acgacgtaga agcggatgcg ccgcaggggc
     4141 tcgcacggga tcggctcgac cgccgccccc ttcgtcgaaa gcggatagag ggttgcccag
     4201 accccgacgc ggcgcgccag cggctccggg ggcatgaccc agccggcgta ccaggccacg
     4261 ccgacgagca gccccccgag caggaggccc gcgagcagcc ggaccgcctt caccgcggtc
     4321 cctccgcggc ggccgccggc gcgcggcgca ccagcagatg gaacggccgg ctggaaaccc
     4381 ggctggtata ggcgaccgac acctccggcg ggagccgctc ccggatcgcc cggtacgact
     4441 cgtcctcgat caaaaggtcc cggtccgggc cgctcccgat ccacgcgagg atcgcgtccg
     4501 gatggtcgag ctcctcgacc gtcgtgtgga gatagaaggc ccacgtctcc cgcttctctt
     4561 cggtcgctcc gagcggcacc gacggatccc gccgcgcctt cacctccgcc gcgaagccgc
     4621 tgaggttgaa gcggcgatcg acctggggca ggacggactc ggcggcgaac agcatgagca
     4681 gtcccatcgc cgcggcgatc ccccacggga cgagccacgt cctccgcctc gccccgcccc
     4741 atgccgcgca cgcggccgag gcggcgagca gcgccgcgat ggtccacgcg ccgaggcgga
     4801 actccggatg atggcgcggc acggccagca ccagcgccag ggccgcggcg catgcgagcg
     4861 gggcgaaggc gagggcggcg cgggcggcgc ggcgcgcgct cccgtcgcgc gtgaagaggc
     4921 gcgcgaccag gagcaccgcc gccggataga gcgggatgat gtagacgccg cgcttgccgg
     4981 tcgagaacga gaagaagagg aagatggcgg cgaaccagac ggtcaggaag acggtcgcgg
     5041 tgcgctcgcg cgcccgggag gacgcggccg gctcgccggc cttgtccggc ctccatgccg
     5101 cgaggagcgc ggccggcagg aacatgcacc agggcaggaa ccccgccggg aacctccaga
     5161 ggtagtacca gacagggtgc tgggagttcc aggcgttgac gtaccggtcg accgtctggt
     5221 gcgtggcgat cccgaccgct tcgctccagc ccagacgcga cgcgaacagc gagaaccagc
     5281 cgccgacgac ggcgagaaag aggatgaccc ccgctgcagg gaagagccgc ggaagatcgc
     5341 gccccctccg cagcagggcg agcgccggaa cgagcgcgag aagcggcagc acgagcccca
     5401 ccggcccctt ggcgagcgtc gcgagtccca tcgcccccca ggcggcgacg gcgcgccagt
     5461 gccaccggct tcctgcgccc tccacctcct cccacagcag gacgaacgcc aggaggacga
     5521 acaggttcag cgtcatgtcg atcgaggccc agcgggcccg ctccaggatc tggagattcg
     5581 tcgcgaacag gacggcgccg aggatccccg tgcggcggtc gtacaggcgc tcgccgagcc
     5641 gggcgacgag caggagcgcc agcatcgagg cgacgagcga cggcatccgg gccgccgtct
     5701 cgtccacctc cccgccgcgc aggatcgccg ccgcgtcgat cgcccagaag aagagcggcg
     5761 gcttgtcggt gtagaggcct ccgttgcgcg acaggacgat cgggtcaccg ccctcgagca
     5821 tctcgcgcgc caccaggccg aaccgcggct cgtcgggcgg ccacagatcg tgcccgccca
     5881 gacgccagcc gaacaggagc agcccgaacg cgaggagcgc cgcgttgaca gcccgccgcc
     5941 ggttcactag gatgcgcgct cccacggggg gatctgcgtg tccgaacgaa gcgccctgct
     6001 cggccgcctg cgcctggccg cgctgttcct gttcgtcatc gccctcgtgg cggtgtcgcg
     6061 gccgacgcgc gccctcctga tcgccggcat ctgcttcgcc gcggcggggg agtcgatccg
     6121 gatctgggcc gctgggcatt tgaggaaatc ggtgcgtctc gccgtctccg gtccctacgc
     6181 tcacacgcaa aatccgctct acctggggcg gctgttgatt ctcaccgggc tggggatcgc
     6241 ggcgcgccac ccgtgggggc tcaactgggc ggcgctcgtc atcggctacg ccatcttctt
     6301 tttctattac atccctcgga agctccgggt ggaaggggcg aggctggagc ggatccacgg
     6361 cgatgcgttc gtgcgctggc gccagagcgt gccgatcctc ttcccctcac ttcgggcgta
     6421 cccgggcggc caggaccgct ggtcgttcgc gcagatggtc cgcaaccagg agccgctcgt
     6481 gctcctcggg cttgttgcgg cgttcgcctg gcttgcgtcc cgcgtcgtcc cctcctgagc
     6541 ccgcgacgac cgcgtcgcgg ccgcgcttgc gcccggccgg ggtcttgagg gtcgcgagcg
     6601 cgtcggcgag ccgggcaccg atccgatcgg ccgagtagac cgcttcgacc cgggcgcggc
     6661 cggcggctgc gaagcggcgt ccacgctccc ggtcctggag cagccggacc atcgcctggg
     6721 tccactccgc ctcaccggtc gcgaacagac cggtcacctc cggctcgacg atctccaggt
     6781 tcgccccgac cggcgagcag acgaccggca cgccggccgc ctggtactgc aggatcttca
     6841 atccgcactt gccgtggctg aaggggcgat cgtcgattgg catcagcccg acgtcgaagc
     6901 cgagcagatc ctcctcctcc cgctcggcgc tccagatcac cttttcggtc ggcacgcccg
     6961 gcaggtcgag gaagtcgttg cacacctgca ccaggcgcgt cccgggcacc cgggcgcagg
     7021 cggctcggag cggggacgcg agggtcgcga ggtagcgcag gctggcgcgg ctgccgatcc
     7081 agccgatacg cgggcctccc gtcgaggcgg cgcggcgggc cgcggcggcg cgcacccgct
     7141 cctccgggaa cgggcgcagg tcgacgccgg tcggcaggat ctcgatgcgc ggagcgcgcg
     7201 cgggcgcgag ccgcgcggcg tgctccgaca ggaagcgatt tcccgccagg acgagcgccg
     7261 cgcgtcgcag cagcgcctgc agccgccggc ggcgggccgc cgcccgccgc tccgctttct
     7321 cctgcccgta gcgggccgct tcggtcgggc tcgcctcgaa gagagcgtcg tcgaagtcga
     7381 acgcgaccgg aaccccctgc gggagcaggt gcagcttcca gggggagaag agcttcttct
     7441 ggatcaggac ggcatcgcag cgtgcggcgt gccgccgcag ctggatcggt ccgtaccctt
     7501 cggtccgctt ggcgacgagg ggctccggct cgatcccgcg ggcgcgcaac cgatcgaggt
     7561 gctgcaggac gcggaaccgg gtgctgggat gatccgcgcc gccgtttaac accaccagca
     7621 gacgcatcgt cgcgcatctt cgcagagacg ccgcggaggg tcaacgcacc gaaaccgggt
     7681 ccggccgggc gtcgttttgg ctgtggtaag gtctcggcat catgcagaac gaaccggaac
     7741 tgtcggtcgt gattccgacg tacgggaagg cggagacgct gccgctggtg atgcgggcgc
     7801 tcgaggggca gtcgatcggg cgcgagcggt tcgaggtcgt ggtgatcgac gacgggtcgc
     7861 cggacgacac gaccgggcgg ctgcgcacgc tggaaggctc gaccccgctg cgcttccgct
     7921 ggatgacaca ggagaaccgc ggcgtgtcgg cgacgcgcaa ccgcggcgcg cgcgaggcgc
     7981 gcgcgccgat cctgctgttc atccaggacg acatcgtcgc ccgccccgat ctgctggagc
     8041 ggcacctggc gatccatgcg cggcatcccg aggcgaccgc cgccggggtc ggccgggtca
     8101 cctggccgcc ggactggtcg atcgatcact tcatggcgtg gctcgacaac ggcggcccgc
     8161 agttccgcta ccaccaggtg gccggacgcg cgaccgtcac cttcaagcac ttctacacct
     8221 gcaacgtgtc gctgaagcgc agagcactcc tcgaccaccc gttcgacgag gagatcgtct
     8281 acggcttcga ggacctcgag ctggcgctgc ggctcgagcg ggccggattc gtcttccatt
     8341 acgacgaaga ggcgctcggg taccaccatc accgccgctc gttcgaggat taccgccggc
     8401 ggcagttcaa ggcggggcag tcgctctacg tcgccttccg caaccacccg gagctcgtcg
     8461 gccggaccgg gatcacctcg atccggccct cgaagcggac gcgcacccgc ctgcgcgggg
     8521 cgatcctgcc ggtggcgcgg ctggtcggcg cgcggcgcac catcgagaag tactggcgcg
     8581 ccgtcctcga cgaggagctg gtggccggct acgagcaggc gcgcgaccgg gatctcagaa
     8641 gaggatccgc accccgcccg gcaccggccg catcgtgacc cgcgccggtc gcgccaccgc
     8701 ctcgcccgga gccccggcgg cgcggtcccg ctccgcgcgg ccgccgcgcc gggagtccac
     8761 caccatccgt ccgacgcaca ggccattgag gtatccgaag tagacgtccg gaagccagtg
     8821 ggcgtcctcg ttgatccgct gcagggccac gagccccgcg gtgccgtaca ggccccaggc
     8881 cccgaaacgc ttccagaagc ggaccgagcc gcggtcctcc ggcctcaccc gaagatgacg
     8941 atcgatgatc ggcgcgagca tcgacgcggc gatcgtcacg tcgagcgaga cgctgtggcc
     9001 gtcgcggtcg aagaaggtca cgtcgtcgcc gtcctccggc cgctcggccg ccaccacata
     9061 gttggcggtc gcggcgatgg cggaggagaa cgccaggctc tccatcagga cgatggcagt
     9121 ctcgcggtcg cgatccgaat cgcgcgccag gccggcgaga taaaagcccc cggcggcgag
     9181 gagcggcgtc gccagccgcc ccatcagccg cgcgtcgtcg agcaggtcgt cccagcgctc
     9241 gccgccgtgc tcgagcgcga cgtcgcgcgc ctcgtcgcgc acgaggtaga gggaggcgcc
     9301 cgccccgact ccgacgccga gccggaccca gccggccgtg tcgaggtggg ccggccgccc
     9361 gaagacgtag cgcgcgtcgc ggccgatctg ccgccagtcg agcggcgcat ccgccgcgag
     9421 cggatcgttc gccgcgactc cggccgcgac gggatcctcc gcagcgggat cctccgcagc
     9481 ggcggccgcg cccgccgtgg ggtcggccgc gaacaggtcg gatgtgaaag cgacggccgc
     9541 gaaggcgatc gccagaccga tccccctcac ggggcgccgt ccgcaggcgc gtcccggggg
     9601 acctccgcgc cgtccccggt ccgccgcacg agcacgaact cacggtggcc gacgcgcccg
     9661 gacaggacga cctccgtacc gtccggagcg gacgcctgtg ccaggtccca ggcggtgcgc
     9721 tcgacgaggc cgacgccccg cccgggggga tcgaggaagc ggaaaagcgc ctcgcgatcc
     9781 ggcagcagcg tgaccggccg tgcgagatac caggcgagtc cggcgtgcgg atcggggtgg
     9841 atgccgatgg gagcctcccc cgtcgcctcc gcgacccgcg ccgcgagcgg ccgcgccgac
     9901 ttcaggggat cgagcgccgg caggacgagg tggacgatga ccaggccggt ggcggcggcg
     9961 ccggcggcaa acacgccgat cgcccggccg gggccgatcc ggcggagcag cagcgccggc
    10021 ggtaggaagg ccatcagccc cgcgatcccg agccaggacc ccgccgtgac caggtcgggc
    10081 gcctcgcggc cggcgcgccg ccagacgacg atcgccgccg cggccacgat cgccgcccag
    10141 caccacaacc cggcgctcac gaggcggcgc gccggcggag ggtcccagtc gaacagtgac
    10201 acatcccagt accgaccgat gagcagcgca atgagtggca ccatcggcaa aaggtacacg
    10261 ccgcgcttct ccaccgaggc ggcgaagatg aggagatcgc tcgcgagcag ggagtagacg
    10321 aacgccaggc gatcgtcgcg cggccgcccg ggccgcggcc aggtctccgc gatcgtcgcg
    10381 gggaggagcg cgatccacgg caggagcgcc agcggcagcg tcgtcaggac gtgccacgcg
    10441 gggcgcggat ggtgcagccc cgacaccacc cgctccgcga accgcccgag cagctccccc
    10501 agcgggaacc gctcgccggt ggaggcgctc caggcgagca tccagaggcc ggccgggacg
    10561 aacagaagtg gcagagagac gatccagtcc ggctgcagca gccggcgcag gcgccgcgtg
    10621 gaggcgaggt acaggagcgc cccggggatc ggcagaagcc ccaccggtcc cttggcgagc
    10681 acgcccaggc cgcacgccag gaagaacccc gcccaccagc cggcccctcc cgcctgggtc
    10741 gcgtgccaca ggcacaggac cgccgccgtc aggaggagcg ccaggagcat gtcgggccgc
    10801 gcccagcgcg cctcgatcag gaagtcctcg gtggtcagca gcacgagcgc cgccagcccg
    10861 ccgcagcgcc gcccgaagag cgaccgccca agggcgaaca gcaccagggc cgtcagaacc
    10921 gccgccgcgt tggccgggat ccgcgccgag gtctcggaga cgccgccgag cgcgctcgag
    10981 gccgcggagg cgagccagta atagaggggc ggcttctcca gccacggatg cccgtgctgc
    11041 tccggcgcgg cccagcggcc gtcgcgcagc agtccggcgg tgatcaccgc ggtgcgcggc
    11101 tcgtccgggt cccacaggtc ccgcccgccg atgccggtgg cgacgagcaa gagcgacgcg
    11161 gcgagcagca cgaggaggac ccgccggtcg gtcgcgaaga gcagcaccgc cggacgcggg
    11221 ccgctcgggc ggtcgttcaa cggacggagg cgcgcggggt cgtgtcgtcg cggaactgca
    11281 ggtggtgcag gcgggcgtac acgcccgacc gggccagaag ctccgcgtgc gctccccgct
    11341 cgacgatccg cccctggtcg agcaccagga tggcgtcggc ccggcggacg gtggacaggc
    11401 ggtgcgcgat gacgaagacg gtgcgcccct gcatgaggtt ctcgagcgcc gcctgcacct
    11461 cattctccga ctcggaatcg agcgacgagg tcgcttcgtc gaggatcagg atcggcgagt
    11521 tcttgaggag ggcgcgggcg atcgacagac gctggcgctg tccctgcgac aggcggtgcc
    11581 ccgactcgcc gagcggcgtg tcgtatccct gcggcagcgc ctcgatgaac gggtgggcgt
    11641 gcgccgcgcg ggccgcctcg atcacccgct cctgcgggat gtcggagcgc ccataggcga
    11701 tgttgttgcg caccgtttcg tcgaacagga tcacctcctg ggtgaccagc ccgatctggc
    11761 tgcgcagcga ggcgagcgac acgtcgcgca cgtcgacgcc gtcgacgagc aggctcccgg
    11821 cggtgacgtc gtagaagcgc ggcagcaggt tgacgagcgt ggacttgccg gcgccgctgc
    11881 tgccgacgat ggcgaccacc tggccgcgat tcacgatcag atcgagagcc tggaggaccg
    11941 gagcccgccc gtaggagaac gacacgccgc gatactcgat ccggtcggag aacggcgcca
    12001 ggtccgacgc ccccggcttc tccaggacct ggggggcctg gtccatgatc tcgaacaccc
    12061 ggcgcgtcgc cgccatcgcc tgctgcagat cgttgttgat cttcacgagg ttgcggatcg
    12121 aggcgaaaat cacgaagagt gcggtcacga acatgccgaa ctcccccgcc gacagtttgt
    12181 cggcggcgat tcggtgccct gcccagacga acagaaccgc agcgccgaac gacccgagcg
    12241 tctccatcac gggcccggtg atcgactgca ggcgggccgc cttcttctcg gcccgctgca
    12301 tgcgatcgag cgcccgggtg aagcgccggg tctcgaagtc ctccatcccg aaggcctgca
    12361 cgatccgcgt gcccgagagc ccctccttca tgatgagggc ggcatccccc atcttctgca
    12421 tgctgccgcg gctcgcctcc ttcagccgcc ggccgatgcg cgctaccggg gcgacgatga
    12481 tcggcagcag gacaaggcag aagccggcca gcctccagtt caggtagaag aggtaggtcg
    12541 cctgcccgag cacgattgcc gcgagccgga agacctccgc caggtccccg gacaccgtcc
    12601 gctgcagccg gctgacgtcc cccatgatcc gggagatcag gtgcccggtg gagtgctccg
    12661 agaagaaggc gagcgactgc cgctggatgc gggcatagag atcgacgcgc aggtccttga
    12721 cagcctgaag cccggtccag cgggtcaggt aggcggtgag gtaggtgaac agccccttga
    12781 acaggaagac gacgacgatg aggatcgcga ccaggaccgc ggtgccccgc gaaccgtcgt
    12841 gcagtgtctg cgaccagcgg gccagaagcg ggaagcggga cgctcccgca tcgatcatgt
    12901 cgcgcgtctt gtcgagcagg tcgaacttgc cggcggtggc ggtggcggtg gcggcgcttt
    12961 tcggcaggac ctcgtcgagg atcggccgca ccagcatcag aagcgccagc cagcacgccg
    13021 agatcaggac cgcagagacg atcgcggcgg ccacccggcg ccagtagggc ttgacgtact
    13081 ggacgagccg cagcaggtcc ttcatggagc cgatctcccc ggcacggggc caatcttacc
    13141 ggggcgggac ctcgagcgcg ctgaagaagt aggcggtttc gaaggccgcc gtctccttgc
    13201 cgtccgaacc gtgcacgatg ttctgctcga tattcgaggc gagatccttg cggatcgtgc
    13261 cggcctccgc cttcgccggg tcggttgccc ccatgatgcg gcggaccgtg gcgatcgcct
    13321 cgggcccctc gacgatcatc ggcacgatcg gaccggaggt catgaacgac accagcgact
    13381 ggaagaacgg ccgctcccgg tgcacgtggt agaagcccga ggcccccgcc gcgtcgagcc
    13441 tggtca
//
LOCUS       DASRIQ010000117         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3167013, whole genome shotgun sequence.
ACCESSION   DASRIQ010000117 DASRIQ010000000
VERSION     DASRIQ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3167013"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..323)
                     /locus_tag="VFD06_02795"
     CDS             complement(99..323)
                     /locus_tag="VFD06_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02495.1"
                     /translation="MTPVWMFEGTRKLDPVAERRLVERRFSALAAAVRDHEARVRRHD
                     SGRARPSDLALYRRLRQILGDHEVAGNARS"
     gene            465..707
                     /locus_tag="VFD06_02800"
     CDS             465..707
                     /locus_tag="VFD06_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02496.1"
                     /translation="MKRFASAVVAAVAIALTLALPAGAGARTIWRCQVPEDGTTVTVD
                     FVSAADAADHGINTANNHAGATFSRNFGEECVVVHD"
     gene            761..1159
                     /locus_tag="VFD06_02805"
     CDS             761..1159
                     /locus_tag="VFD06_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN02497.1"
                     /translation="MSQDKVEIVQQIFDSWARGDFGAGLTDLDPDVVFVVRHPFPEAV
                     RTVGPDGIGRYLRRFLDNWETYAVKASDFRPAGDTVVVDAVQHGRGKASRIDMEQRFY
                     MLFTFRGPKIVRIESILERDQALAAAGLRE"
     gene            1163..1585
                     /locus_tag="VFD06_02810"
     CDS             1163..1585
                     /locus_tag="VFD06_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN02498.1"
                     /translation="MSQENVEFLQGLFAGAADLDKQELLAALPELIAQTCDPEIEWVE
                     DPQRADRRIYHGHEGVRESWERWLENFDEYGGEVERMVDCGDKVLVVAREAGRGRASG
                     ATISQLIYTVFSFRDGKIARYEEFYDERAALEAAGLRE"
     gene            1737..2180
                     /locus_tag="VFD06_02815"
     CDS             1737..2180
                     /locus_tag="VFD06_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN02499.1"
                     /translation="MSPENVEIVRRVLDLMEEGVRSHDVRTAIDEGIAAGLISPECEV
                     RGGRRGGASVVGLGDEVGREGMVDFMRTWAEDFSDFAFEVEEVIDAGENGVLATQRQA
                     ATGRASGAPVEMHTATIFRLESRRVVCIEIFVNPAEALEAAGLRE"
     gene            complement(2227..>2620)
                     /locus_tag="VFD06_02820"
     CDS             complement(2227..>2620)
                     /locus_tag="VFD06_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02500.1"
                     /translation="GLSDGLACSQMRGSRGWRYLAAAFWIANGLNHFRAPRFYEAIVP
                     PPFQQMRREVNVGSGAAELIGGLAVLPDRTRPAARWWLLTTLLAIYWANVHMALRPDR
                     FPRFPPALLYARLPLQGLFAWLTWRGTQ"
BASE COUNT          459 a          814 c          929 g          418 t
ORIGIN      
        1 cgggcctcgc gcgttgcgtg gtttggggtg tggagaggcg ctattcgggg gaaggcaaag
       61 cgaccgcggg gagcgaccgg agccaatcgc gcgcgccctc aggatcgggc atttccggcg
      121 acctcgtggt cccccaggat ctgccgtagc cgccggtaga gggcgagatc cgacggccgt
      181 gcccttccgg agtcatgccg acggacgcgg gcctcgtggt cacgcaccgc agccgcgagc
      241 gcggagaagc gccgctccac gagcctccgc tcggcgaccg gatcaagctt gcgcgttccc
      301 tcgaacatcc agaccggagt cactgatctc gatcctcctt cctcagtttg ttgaccgatt
      361 agatcagaat atcacctaac ggaccctggg gcgtcttccg cgaaggagac gcggagggct
      421 gaccccgggg tatcgtttcg gtgtcgaggg aaggaggttc ccgaatgaag cgattcgcct
      481 ctgcggtcgt tgcggcggtt gctatcgcgt tgacgctcgc gctgcccgcc ggagccggag
      541 cgagaacgat ctggagatgc caggtccctg aggacgggac gacggtcacc gtggacttcg
      601 tgtccgccgc cgacgccgct gatcacggca tcaacacggc caataaccat gcgggcgcga
      661 cgttcagccg caatttcggt gaggagtgcg tcgtcgtcca cgactgaccc gtttgctggg
      721 cttgccctgc ggggtcggat ggacggatac tgcgtaggcg atgtcgcagg acaaggtcga
      781 gatcgtccag cagattttcg acagctgggc cagaggcgat ttcggcgcgg gcctcaccga
      841 tctggatccc gacgtggtct tcgtcgttcg ccatccgttc cccgaggcgg tcaggaccgt
      901 cggtccggac gggatcggcc gctacctgcg ccgcttcctg gacaactggg agacctacgc
      961 ggtcaaagcc agcgacttcc ggccggccgg tgacaccgtg gtggtcgatg cggtccagca
     1021 cggcaggggc aaggcgagcc ggatcgacat ggaacagcgg ttctacatgc tgttcacctt
     1081 ccggggacca aagatcgtgc gaatcgagtc cattctggaa cgagaccagg ccctcgcggc
     1141 ggccgggctt cgggagtagg cgatgtcgca ggagaacgtc gagttcttgc agggcctctt
     1201 cgcgggcgcc gccgacctgg acaagcagga gctgctcgcc gccttgcccg agctgatcgc
     1261 gcagacctgc gaccccgaga tcgagtgggt ggaggacccg cagcgtgcgg accggcgcat
     1321 ctaccacggc cacgaaggcg tccgcgaatc ctgggagcgc tggctcgaga acttcgacga
     1381 gtacggcggc gaggtcgagc ggatggtcga ctgcggggac aaggtgctcg tggtcgcccg
     1441 cgaggcaggc cgcggaaggg cgagcggggc gaccatcagc caactgatct acacggtgtt
     1501 cagcttccgc gacggcaaga tcgcccgcta cgaggagttc tacgacgagc gcgccgcact
     1561 cgaagccgcc ggcttgcgcg agtaggcgac gtcgaggagg accgctaacc cttcaatggc
     1621 cgctgctccc ggcagcgatt gaagacttag cctctctcag aggggtcaac tcttcaatcg
     1681 cttgcgccgc acggggcgag tgaagagtta gcgggcatag cgggatactg agcgggatgt
     1741 cgcccgagaa cgtcgagatc gtccgtcggg tcttggatct catggaagag ggcgtgcgga
     1801 gtcatgacgt ccgcaccgca atcgacgagg gaatcgccgc cggactcatc tctccggaat
     1861 gcgaggtgcg cgggggccga cgcggaggcg cttccgtcgt cggactcggc gacgaggtcg
     1921 gacgggaggg catggtggac ttcatgcgca cgtgggctga ggatttctcc gactttgcct
     1981 ttgaggtcga ggaggtcatc gacgcgggcg agaacggggt gctggcgacc caacgtcagg
     2041 ccgcgaccgg cagggcaagc ggcgccccag tggagatgca cacggccacg atcttcaggc
     2101 tcgaatcccg gcgggtcgtg tgcatcgaga tcttcgtgaa cccggcggag gccctcgaag
     2161 ccgccgggct ccgggagtag acggttccgc cgaacccttt cggtggatat cgcccggccc
     2221 gacgcgctat tgcgtcccgc gccaggtgag ccaggcgaac aggccctgca gggggagtcg
     2281 cgcgtacaag agggctggtg ggaagcgcgg gaaccggtcg ggccgtaacg ccatgtgaac
     2341 gttggcccag tagatcgcca gcagagtcgt cagcagccac cagcgggcag ctgggcgcgt
     2401 gcggtcgggc agcacggcca acccgccgat cagctcggcc gcacccgagc ccacgttgac
     2461 ctcgcgccgc atctgctgga acggaggcgg gacgatcgcc tcatagaacc tcggagcgcg
     2521 gaagtggttc agcccattcg cgatccagaa tgcggcagcc aggtatcgcc atccccgtga
     2581 cccgcgcatc tgtgagcagg ctaaaccgtc gctgagaccg
//
LOCUS       DASRIQ010000118         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3701673, whole genome shotgun sequence.
ACCESSION   DASRIQ010000118 DASRIQ010000000
VERSION     DASRIQ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3701673"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VFD06_02825"
     CDS             <1..1314
                     /locus_tag="VFD06_02825"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZN02501.1"
                     /translation="HFTGDFHAVATAHNLLAALVDNHLFQGNSLGLDPDAILWRRSLD
                     LNDRALRRVRIGVGGGTNGVERETGFDITASSEVMAILALSTGIADLKDRLGRIVVGL
                     NREGRPVRARDLRAAGSMAAVLKDAIQPNLVQNLEGGGAIIHCGPFANVAHGNNSILA
                     DRLALKLADYVVTESGFGSECGAEKLFDIKCRVSGTAPAAAVIVCSVRAVKLQGGATI
                     DTKDAATWKRPDPEAVARGGANLAKHIENIRLFGLPAVVAINRFDSDSPEEVAAVRKA
                     ALEAGALAAVPVEVWAKGGEGGIELGEAVLKACETPSRFKPLYELDRSIEEKIETIAT
                     KVYGAAGVSYSDRAKERIAFLRREGFEGLPICMAKTPLSLSHDPKIKGRPEGFVFPIE
                     EVRIAAGAGFIYPLAGSIMTMPGLPPKPAAESIDLDDAGLVHGLF"
     gene            1549..2316
                     /locus_tag="VFD06_02830"
     CDS             1549..2316
                     /locus_tag="VFD06_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HZN02502.1"
                     /translation="MLALFATLVSSSVASAATLRLSWSSDSEADLSGYRLRYGTASGA
                     LDYEVDAGRAKSVDVIGLLRGVTYYFSVFAYDASGNESAPSAEVMARLTTDLSAPPIV
                     ESAMEMTSGSIYAVRLFAHLIMIKGQNFEPGATVDLGDGIVTAPPIRTATGDLVVRVT
                     VPANAPPGPRSVTVFNPDLGVGSGTEMLTIVKSPDSNADCTVDIMDLNTLARAWNEET
                     GEARYAPTVDLDGDDYVGPEDLTIFVSYFGREFTGCP"
     gene            2362..2964
                     /locus_tag="VFD06_02835"
     CDS             2362..2964
                     /locus_tag="VFD06_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02503.1"
                     /translation="MIVRYDLHRRASILTLGLCAALVLGGCGGGGGGDDDDSPTCTNL
                     SFDRAMVTPGVGDIYMDQASSTCSTLDVVVLVSNLSGIFTVGFDLTYPTSALQYQSYS
                     VGPLMLKGNPTQPPLVLVSPLATGIQITMTRFGSDPPVSATGSEALISLRFVRTGSGV
                     GAFDFNTGAGDLVTETVLDQNGASRPAVWTPNHGGMVAIP"
     gene            2964..>3349
                     /locus_tag="VFD06_02840"
     CDS             2964..>3349
                     /locus_tag="VFD06_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02504.1"
                     /translation="MNRRPGAWISACAALLAIALAAAPVWDAWRYRRLRSPVGPHVPV
                     GVILPLRSAPPETAPPGGAPALSGEAAPIARTATYASKDAASGAPTGQSPSPTAAATH
                     RTAGTPTVATTPASTVRRADGTPGHRP"
BASE COUNT          502 a         1214 c         1100 g          533 t
ORIGIN      
        1 cacttcaccg gcgacttcca cgccgtcgcc acggcgcaca acctgctcgc ggcgctcgtc
       61 gacaatcacc tgttccaggg gaactcgctc ggcctcgatc ccgacgcgat cctctggcgg
      121 cgcagcctcg acctgaacga ccgggcgctc cggcgcgtgc gcatcggggt cggcggcggg
      181 acgaatgggg tcgagcggga gaccggcttc gacatcaccg cctcctcgga ggtgatggcc
      241 atcctggcgc tctccaccgg catcgccgac ctcaaggatc gcctggggcg catcgtcgtc
      301 ggcctgaacc gcgagggccg ccccgtccgg gcccgcgacc tcagggcggc cggctcgatg
      361 gcggcggtcc tcaaggacgc gatccagccg aacctggtgc agaacctcga ggggggcggg
      421 gcgatcatcc actgcggccc gttcgccaac gtcgcgcacg gcaataactc gatcctcgcc
      481 gatcgtctgg cgctgaagct cgccgactac gtcgtcaccg agagcggctt cggatcggag
      541 tgcggcgcgg agaaactctt cgacatcaag tgccgcgtca gcggcacagc tcccgccgcg
      601 gcggtcatcg tctgctcggt ccgggccgtc aagctgcagg ggggggccac catcgacacc
      661 aaggacgccg ccacctggaa gcggcccgac ccggaggcgg tggcgcgcgg aggcgccaac
      721 ctcgccaagc acatcgagaa catccgcctg ttcggcttgc cggcggtcgt ggcaatcaac
      781 cgcttcgact ccgacagtcc cgaagaggtc gccgcggtgc gcaaggcggc gctcgaggcc
      841 ggggccctgg cggccgtgcc ggtcgaagtc tgggccaagg gcggcgaggg agggatcgag
      901 ctcggcgagg cggtgctcaa ggcctgcgag acaccgtccc gcttcaaacc gctgtacgag
      961 ctcgaccggt cgatcgagga gaagatcgag acgatcgcga cgaaggtgta tggcgccgcc
     1021 ggcgtgtcgt acagcgaccg ggcgaaagag cggatcgcgt tcttacgccg cgaaggattc
     1081 gagggactgc cgatctgcat ggcgaagacc ccgctctcgc tgtcgcacga tcctaagata
     1141 aaggggcgcc ccgagggctt cgtgttcccg atcgaggagg tccgcatcgc cgccggcgcg
     1201 ggattcatct atccgctcgc cggcagcatc atgacgatgc ccgggctgcc gccgaagccg
     1261 gcggcggaat cgatcgatct cgacgatgcc gggctcgtgc acggtctctt ctaggaccct
     1321 gtccgagtcg catcgcaccg ctctccgggc ttccgagccc gcgctccgcg tttcactcag
     1381 cggtgctgtc cgccgcgtcc tgaccccgat ctccgcagtt tctggcacca cccttgctac
     1441 ggcacatgcc gtgacgcatc cgcgcgcagt cgccgcgcgc gagttcgcgg agaggggatg
     1501 gagcggagcc tcccagcagc agtccccaaa tcgctgatag tcgcagggtt gctcgctttg
     1561 ttcgcgacgc tcgtgtcgtc gagcgtcgcg tcggcggcga cactgcggct ttcgtggtcg
     1621 tccgattccg aggccgacct ctccggctac cgtctccgct acgggacggc ctcgggtgcc
     1681 ctcgactacg aggtggacgc cggccgcgcc aaaagtgttg atgtcatcgg ccttcttcgc
     1741 ggcgtgactt attatttttc cgtcttcgcc tacgacgcct ccggcaacga gagcgcacct
     1801 tcggcggagg tgatggcgcg cctgacgacc gacctcagcg cgccgccgat cgtcgaatcg
     1861 gcgatggaga tgacgagcgg ctcgatctac gcggtgcgat tgttcgccca cttgatcatg
     1921 atcaaaggcc agaacttcga gcccggcgcc acggtggacc tgggggacgg gatcgtcacc
     1981 gccccgccga tccgcaccgc caccggcgat ctcgtcgtcc gggtcacggt tcccgccaac
     2041 gcgccccccg ggccgcgctc cgtcaccgtc ttcaatcccg acctcggtgt cggaagcggc
     2101 accgagatgc tcaccatcgt caagtccccc gactccaacg ccgactgcac cgtcgacatc
     2161 atggatctca acacactggc ccgcgcgtgg aacgaagaga cgggggaggc gcgttatgcg
     2221 cccacggtcg acctcgacgg tgacgattat gtcggacccg aggacctgac gattttcgtc
     2281 agctacttcg ggcgggaatt cacgggctgc ccttgaccgg gccggaccgg gccacatgtt
     2341 tcgaaggtgg ggaggaatcc tttgatcgtt cgttacgatc tccaccgtcg cgcctccatc
     2401 ctcacgctcg gtctgtgcgc cgccctcgtg ctcggcggct gcggaggcgg cgggggcggc
     2461 gacgatgacg actcgcccac ctgcaccaac ctctccttcg accgcgccat ggtgaccccc
     2521 ggcgtcgggg acatctacat ggatcaggcc tcctccacct gctcgaccct cgacgtcgtc
     2581 gtcctggtgt cgaacctgag cgggatcttc acggtcgggt tcgacctcac ctatccgacg
     2641 tccgctcttc aataccagtc gtactccgtc ggaccgctga tgctgaaagg gaatccgacc
     2701 cagccgccgc tcgtgctggt ctccccgctc gcgaccggca tccagatcac catgacccgc
     2761 ttcggatccg atcctccggt gtccgcgacc ggcagcgagg cgctcataag cctgcgcttc
     2821 gtgcgcaccg gatcgggcgt cggcgcgttc gacttcaaca ccggcgccgg ggacctcgtg
     2881 accgagacgg tgctcgatca gaacggcgcc tcccgccccg ccgtctggac gccgaaccac
     2941 ggcggcatgg tggcgatccc ctgatgaacc gccgccccgg cgcgtggatc tcggcctgcg
     3001 cggcgcttct cgcgatcgcg ctcgcggcgg cgcccgtctg ggatgcctgg cgctaccgga
     3061 ggctgcgctc ccccgtcggc ccgcacgtgc cggtcggggt gatcctgccg ctccggtcgg
     3121 cgcctcccga aacggcgccg cccgggggcg cgccggccct gtcgggcgag gcggcgccga
     3181 ttgcgcggac cgcgacgtac gcatcaaagg acgcagcgtc cggtgctccc acgggccagt
     3241 ctccctcgcc gaccgccgcg gcaacgcacc ggaccgccgg cacgccgacg gtggccacga
     3301 cgccggcctc aacggttcgc cgtgccgacg ggacgccggg tcaccggcc
//
LOCUS       DASRIQ010000119         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3167195, whole genome shotgun sequence.
ACCESSION   DASRIQ010000119 DASRIQ010000000
VERSION     DASRIQ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3167195"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFD06_02845"
     CDS             <1..1179
                     /locus_tag="VFD06_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02505.1"
                     /translation="RSGFRAVLLPTVLAFAMTALVAAVRCGGWGRFVAAGALFGLLVH
                     TYPSARAAPLLLPPYLLAEFGFDRARWRQAAPGLVLFALLALLVASPMLLHYVHHPED
                     FNNPNCIVSVFSPGLAPGEANAHLKRNVAATLLMFHVRGDANWRHNISGAPMLDPLTG
                     VLFLGGLLLAFRMVFGKEIEGAERPRAAAVLLLAWLFGMLLPNLLSVEGVPHGLRSAG
                     ALPAVVLLAGLAVSWIGERAAARRLLPARAASGLAVGVALLLAVACGWRYAVTWGKDP
                     AVFLEHDGALRAAAKALLTAPPGAERFLVANGSGYRVQGWPAEAAPYRFEMRSAPPVL
                     LGSKDAARLALSGRTAYVALIAADSKVIEVIRTLNPGAPITAIHAPGVEPTSPVYRVN
                     "
     gene            complement(1213..>2342)
                     /locus_tag="VFD06_02850"
     CDS             complement(1213..>2342)
                     /locus_tag="VFD06_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZN02506.1"
                     /translation="TGGLDEDGGQVGDGDPEGFDARRGQAALATADLEGIAETVGDLP
                     IVAHRVSREVEPLRRAAALAGIPFHPRVISAARLGHLLCGLKANHLLQELAALLGVPA
                     DGPDDCRGRARLLSRVWEALVPALAAQGVETIDGLLEFQELASPPIDFAPYAFTAEEL
                     RLLPSSPGVYRFFDAGGATIYIGRAKNLRSRVGSYFVPSARRTPKGRAILERVRRLEI
                     ELVGSELEAILLEADLLLKERPPLNRQFEVSERPAPYGPRQNLVVVLRDADGKACTAH
                     FLRGGRYLRRLPCVGDAAARDSGWETIDAEVRRSYFTPATEEEQGGEIDWFLLCSYLR
                     AHADELSVLDVDECAGADDALARLRVLHDAARAGAGRVLAR"
BASE COUNT          295 a          867 c          835 g          345 t
ORIGIN      
        1 cggagcggct tccgggccgt cctgctgccg accgtgctcg ccttcgcgat gacggcgctg
       61 gtcgcggcgg tgcgctgcgg cgggtgggga cggttcgtgg cggcgggagc gctcttcgga
      121 ctcctggtgc acacctaccc gtccgcccgg gccgcgccgc tcctcctgcc gccctacctt
      181 ctcgcggagt tcggcttcga tcgcgcccgc tggcgccagg cggcgcccgg gctcgtgctc
      241 ttcgcgctcc tggctctgct cgtggcgtcg ccgatgctcc tccactacgt gcatcacccc
      301 gaggacttca acaacccgaa ctgcatcgtc tccgtcttca gccccgggct cgcgccgggg
      361 gaggcgaatg cacacctgaa gcggaacgtg gccgccaccc tcctgatgtt ccacgtgcgc
      421 ggcgacgcga actggcggca caacatctcc ggcgccccga tgctcgatcc cctgaccggc
      481 gttctcttcc tcggtggtct gctgctcgcg ttcaggatgg tgttcgggaa ggagatcgag
      541 ggggcggagc gcccgcgcgc ggcggcggtc ctcctcctcg cgtggctttt cggcatgctc
      601 ctgccgaatc tcctctccgt ggaaggggtc ccgcacgggc tgaggagcgc cggggcgctg
      661 ccggcggtgg tcctcctcgc cggtctggcg gtgtcgtgga tcggggagcg ggccgcggcc
      721 cgccgactcc tcccggcccg tgccgccagc gggctcgctg tgggtgtcgc gctcctgctc
      781 gccgtcgcgt gcggctggcg ctacgcggtg acctggggga aggatcccgc cgtcttcctc
      841 gagcacgacg gggcgctgcg cgccgcggcg aaggcgctgc tcacggcacc gcccggggcg
      901 gagcgcttcc tcgtcgccaa cggcagcggc taccgcgtgc agggctggcc ggccgaggcg
      961 gccccgtacc ggttcgagat gcgctcggca ccgccggtgc tgctcgggtc gaaggacgcg
     1021 gcgcgcctgg ccttgtccgg gcggacggcc tacgtcgctc tcatcgccgc cgactcgaag
     1081 gtgatcgagg tgatccggac tctgaacccc ggcgcgccga tcaccgcgat ccacgctccg
     1141 ggcgtcgagc cgacgagccc cgtctatcgt gtgaattagg acgaggtgtc gatcgcgtga
     1201 atcgagacga tctcagcgcg ccagcacccg cccggccccc gcgcgcgcgg cgtcatgcag
     1261 gacgcgcagc cgcgccagcg catcgtccgc gccggcgcac tcgtcgacgt cgaggacgct
     1321 cagctcgtcc gcatgggccc ggagatagga gcagagcagg aaccagtcga tctccccgcc
     1381 ctgctcttcc tccgtcgcag gggtgaagta ggaccttcgc acctccgcgt cgatggtctc
     1441 ccagccggag tcccgggcgg ccgcatcgcc gacgcagggg agccggcgca ggtaccggcc
     1501 gccgcgcagg aagtgcgcgg tgcacgcctt cccgtcggcg tcccgcagca cgaccacgag
     1561 attctggcgg gggccgtacg gggcggggcg ctccgagacc tcgaactggc ggttgagcgg
     1621 tggccgttcc ttcaggagaa ggtccgcctc cagcaggatc gcctcgagct cggagccgac
     1681 gagctcgatc tcgaggcgtc gcacccgctc gaggatcgcc cgccccttcg gagtgcgccg
     1741 tgccgagggg acgaaatacg agccgacgcg gctcctgagg ttcttcgctc tgccgatgta
     1801 gatcgtcgcg ccgccggcat cgaagaaccg gtacaccccc ggcgaagacg gcaggaggcg
     1861 caactcctcg gcggtgaagg catagggggc gaagtcgatc ggcggtgacg ccagctcctg
     1921 gaactccagg agcccgtcga tcgtctcgac cccctgcgcc gcgagcgccg gcaccagcgc
     1981 ctcccagacg cgcgacagca ggcgcgcgcg gccgcggcaa tcgtccggac cgtccgccgg
     2041 cacgccgaga agcgccgcca gctcctgaag gaggtgattc gccttcagcc cgcagagcag
     2101 gtgtccgagc cgtgcggcgg agatcacccg ggggtggaag gggatgccgg cgagcgccgc
     2161 ggcgcggcgt aacggctcca cttcccgcga cacccggtgc gccacgatcg gcagatcgcc
     2221 gaccgtctcg gcgatcccct cgagatcggc ggtcgcgaga gcggcctggc cgcggcgcgc
     2281 gtcgaagccc tccgggtcgc cgtcaccgac ctggcctccg tcctcgtcga gcccccccgt
     2341 gg
//
LOCUS       DASRIQ010000120         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_51036, whole genome shotgun sequence.
ACCESSION   DASRIQ010000120 DASRIQ010000000
VERSION     DASRIQ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51036"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..1066)
                     /locus_tag="VFD06_02855"
     CDS             complement(374..1066)
                     /locus_tag="VFD06_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN02507.1"
                     /translation="MVSPLVLVVDDEPHICLLVRESLRQAGMTVRTASDGRQALRALD
                     EDDPALILLDVGLPDIDGFSLCSRIREVSHAPIIMLTARHHDEDVVRGFKAGADDYVI
                     KPFSPRQLAARAEAMLRRSRGPSSAIGRYQDDQLEVDVSARRSVLHGVSTRLTPSEAK
                     LMDLVLAHPRRVFTPQELLRHLRGPGYEDAHGALRQVILGLRRKIEPDPSSPTYLVTA
                     PGGGYAFEPRTD"
     gene            complement(1352..1954)
                     /locus_tag="VFD06_02860"
     CDS             complement(1352..1954)
                     /locus_tag="VFD06_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN02508.1"
                     /translation="MQAAAELVVLAQRGDQAAFGSLYEQCAPLVYRFVRRRLDGDDEI
                     VQDLTEDVFVKAYEKLDRYVERGLPFTAWLYRIAHNHLVDYLRTLPRLCASSLDAVAE
                     VPERAASAAFSRVLDQQSLEPALARLTPEQRQAVELRFIEGMSVAETAETMGRSDEAV
                     KKLQARALANLRRHLAPPAAAPTGSPLGRRTGNALRMAVA"
     gene            complement(2182..>3513)
                     /locus_tag="VFD06_02865"
     CDS             complement(2182..>3513)
                     /locus_tag="VFD06_02865"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02509.1"
                     /translation="LRRYGGNGVEAQIARRLGLRSIVAAPLRGARGPVGAICVGWTSD
                     RRRPDDQDAHLVDELARRASLALEQARCLRALEQSLERIELVLNSTGAGLFIFGGDGR
                     AILVNATACAMIGLDGGGEGDGDGVGQTLAEILSPYEERFEDTGDLDDIVHRAADLGT
                     DSSGELRLRAPEPTDLHWIATPVRQPDGPVLGQVVVWVDVTHIRAAERVKDALAADLS
                     EALRLPLQAVSTHAVQALRRGRRAGTDPTLLHGLETILRNARQISMHVNDLVDAARFD
                     ASTLTLDLIEVDVHEVLQRAVDQARAMTTTHRFRIDIPPAVPPPRWDPDRVRQALLHI
                     LSNAIKYWPEGGQITIKARPQVEGVVVSVRDRGLGVPPAEQERIFERYFRLADHPARH
                     RIRGNGLGLYLVRGVVDAHRGTVWIESTGVPGEGTTVHLLLPWLPTAAQPR"
BASE COUNT          492 a         1241 c         1291 g          489 t
ORIGIN      
        1 cacgtaccgc gccggatgct ccgccgggat cagctcctcc agcgtcggcg gcagcagcca
       61 cgtctgctcc cgactgaact cccgcagcgg catcgctcca ccccctcgac ggcctaccgc
      121 cagcgtaccg cagaccgaca cacccccggt agcggaacag gagttctgtc acaggctcgt
      181 cagcgagccg gactcagtgt cgggtccggc tcgctgacgc tcgcggtgcg gaacggagcg
      241 cccggtgcgg aatggagcgc ccggtgcgga atggagcgcc cggtgcggag ctgagcgcgt
      301 acgggttgac gccccacctc caagcggtgg caggcctgcg gaacggagca cccggcccgg
      361 gagacgcagc cggtcagtcg gtgcgcggtt cgaacgcgta gcccccgccc ggcgccgtca
      421 cgaggtaggt gggcgacgac gggtccggct cgatcttacg ccgcaagccg aggatcacct
      481 gccggagcgc cccatgggcg tcttcgtagc cgggacccct gaggtgacgc agcagctcct
      541 ggggcgtgaa cacccgccgc gggtgcgcca gcaccaggtc catcagtttc gcctcggagg
      601 gggtcagccg ggtgctgacg ccgtgcagga cgctccgtcg cgccgagacg tcgacctcga
      661 gctgatcgtc ctggtaccgc ccgatcgccg aggacggccc gcgcgagcgg cgcagcatcg
      721 cctcggcccg cgccgcgagc tggcgtgggc tgaacggctt gatgacgtag tcgtcggcgc
      781 cggccttgaa tcctctgacg acgtcctcgt cgtggtggcg cgccgtcaac atgatgatcg
      841 gcgcgtggct gacctcgcgg atgcgcgagc agaggctgaa gccgtcgatg tccggcaggc
      901 cgacgtccag gaggatcagg gccggatcgt cctcgtcgag cgcgcgcagg gcctgccgac
      961 cgtccgaggc cgtgcgcacg gtcatcccgg cctggcgtag cgactcgcgt acgagcaggc
     1021 agatgtgcgg ctcatcgtcg acgacaagta cgagcggtga aaccatctga gcaggcgcgc
     1081 ctcgcgagcg tcgggactca ttgagcatct gcgagtacgg aagagcgagt gcgcgaaccc
     1141 ggcgccagag cagtttaaca cacccggcgc ccgccctcat gtcgaaactc gccgtcaccg
     1201 ccatcccgcc atcgagcggt ggcgcggccg gcaacgcgag ggccccctcg gcgggtcgtc
     1261 gagggggcct cgttgagatc cggggctggg tttcgggcgt cgccgcgcga aggtccggga
     1321 cgagacggcg cggcggcccc gcggagcggg atcaggccac ggccatccgg agcgcgttgc
     1381 cggtccggcg gccgagtgga gaccccgtcg gggcggcggc cggcggcgcc aggtggcggc
     1441 gaaggttcgc gagcgcgcgg gcctggagct tcttcacggc ctcgtcggag cgccccatcg
     1501 tctcggcggt ctcggcgacc gacatcccct cgatgaaccg cagctcgacg gcctggcgct
     1561 gctccggggt cagccgggcg agggccggct ccagcgactg ttgatcgagc acccggctga
     1621 aggcggcgct ggcggcgcgc tccgggacct cggcgaccgc gtccagcgag gaggcgcaca
     1681 ggcggggcag ggttcgcagg tagtccacca ggtgattgtg ggcgatgcgg tagagccagg
     1741 cggtgaacgg caggccgcgc tcgacgtagc ggtccagctt ctcgtaggcc ttgacgaaga
     1801 cgtcctcggt caggtcctgg acgatctcgt cgtcgccgtc gagccggcgg cgcacgaagc
     1861 ggtacaccag cggcgcgcac tgctcgtaca gcgatccgaa ggccgcctga tcgccccgct
     1921 gcgccaagac caccagctct gccgctgcct gcatggccgt tccccttatc ccgtctctct
     1981 cgcctgccgt ccggcgtgtc ccgcgtgccg ctcggccgtg agccaagtct gacacgggtc
     2041 ccgtgagacg cacgtcagga acgtgaggac cggcgtcagg ccggcgttag atttctgagg
     2101 aaggccgggc cgaaacgggc gcgagttgca ggaacctccc ctccccaggc attggggagg
     2161 ggaatgcatc ggcgggctcc ctcagcgggg ttgggcggcg gtcggcagcc agggcagcag
     2221 caggtggacc gtcgttccct cgccggggac gcccgtcgac tcgatccaga ccgtcccccg
     2281 gtgcgcatcg acaaccccgc gcaccaggta cagcccgagg ccgttgccgc ggatgcgatg
     2341 gcgcgcggga tggtcggcga ggcggaaata ccgctcgaag atccgctcct gctccgccgg
     2401 cggcaccccg agacctcggt cgcggacgct gacgacgacc ccttcgacct gggggcgggc
     2461 cttgatggtg atctggccgc cctccggcca gtatttgatg gcgttgctca ggatgtgcag
     2521 cagcgcctgg cgcacccggt ccgggtccca gcgcggcggc ggcacggccg gcgggatgtc
     2581 gatgcggaag cggtgggtag tggtcatcgc gcgggcctga tcgaccgccc gctgcaagac
     2641 ctcgtggacg tctacctcga tcaggtccag cgtcagcgtc gacgcgtcga agcgggccgc
     2701 gtccaccagg tcgttgacgt gcatcgagat ctggcgcgcg ttcctgagga tggtctcgag
     2761 gccgtgcagc agcgtggggt ccgtgccggc ccgtcggccg cgccgcagcg cctggacggc
     2821 gtgcgtggac accgcctgga gcggcagccg tagcgcctcg ctcaggtcgg ccgcgagggc
     2881 gtccttgacg cgctcggcgg cccggatgtg ggtgacgtcc acccagacga ccacctgacc
     2941 gaggaccggc ccgtccggtt gccgcaccgg cgtggcgatc cagtgcaggt cggttggctc
     3001 gggcgcccgc aggcggagct cgccgctcga atcggtgccg agatcggccg cccggtgcac
     3061 gatgtcgtcg aggtcgccgg tgtcctcgaa ccgctcttca tacggcgaca ggatctcggc
     3121 gagggtctgc ccgacgccgt cgccgtctcc ttcgccgccc ccgtcgagcc cgatcatcgc
     3181 gcacgcggtc gcgttgacca ggatggcgcg cccgtcgccg ccgaagatga acagcccggc
     3241 cccggtcgag ttcagcacca gctcgatccg ctccagcgac tgttcgagcg cgcgcaggca
     3301 gcgcgcctgc tcgagcgcca ggctggcgcg gcgggccagc tcgtcgacga ggtgggcgtc
     3361 ctggtcgtcc gggcggcggc ggtcgctcgt ccagccgacg cagatcgccc ccaccggccc
     3421 ccgcgcgcct cgcagcgggg ccgcgacgat cgaacggagg ccgagccgac gggcgatctg
     3481 ggcctcgacg ccgttcccgc cgtagcggcg cag
//
LOCUS       DASRIQ010000121         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2920833, whole genome shotgun sequence.
ACCESSION   DASRIQ010000121 DASRIQ010000000
VERSION     DASRIQ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2920833"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..377)
                     /locus_tag="VFD06_02870"
     CDS             complement(18..377)
                     /locus_tag="VFD06_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02510.1"
                     /translation="MSRFVWVGVLLTVLLAQAAPAASCLTGQAPSMPCCKPGSDCASD
                     GLNVPSCCGLDAPADRQEDADRHHGAPVPSPDRHVVMVPNAPAADVVLCGSCEAAGSP
                     PREPGIPIYLRLGALLR"
     gene            445..918
                     /locus_tag="VFD06_02875"
     CDS             445..918
                     /locus_tag="VFD06_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN02511.1"
                     /translation="MTFLALLVLALAAATPAPAAVPVLSVSGAFFALSVPDARKSASW
                     YGEKLGLTVVMDPPKHGKSKVIVLEGGGLIVELIQEDDAVPLGATAPGARSPMQVHGI
                     AKAGVIVSDFEATLAALRQKKVEIAYGPYPAGKGQRANCIIRDGDGNLIQLSGRS"
     gene            1063..>3293
                     /locus_tag="VFD06_02880"
     CDS             1063..>3293
                     /locus_tag="VFD06_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02512.1"
                     /translation="MARSRAGVYGISIAAGGAAILLRFILDPWLGNQQPYSIVYAAVA
                     LAVWLGGYRPAGVAALVAFLPATWLFVEPRQVMGHVGPTAALGFFLYLATCGIVIAFG
                     EAMRRASQRAEAAREEAQRHSRLLQESENRFRALADSAPVSIWMDGPTGDCILVNRPY
                     LEYTGLRRDQVEGPGWVDVVHPDDSARYVGAFREALAHRSEFHQEVRLRGHDGTYRSF
                     EVFARPRFEGDRFLGHVGICIDVTERKAAQATAAMSDERLASELEAMSRLHALSSRLM
                     SATDLNVALEDVMENAMATCGADFGNIQVIQPTSGALEIAAQRGFRSEFLEHFRAVGA
                     GDASACARALRQGAACLVEDVQLDPDFAPHRGIAAAAGFRAVQSTPLVTHDGMILGML
                     STHFREPHRLSERDRRFLDLYARLAADLIERLRYERTLEQADRRKDEFLATLAHELRN
                     PLAPIRNAVHVFKAKGPADPDLTWSRQVIERQLGQMARLLDDLLDVSRITRNTFDLRR
                     ASVPLAPVLANAVETSRPLIEEARHELIVEVPAEPIYLEADPVRLGQVFSNLLNNAAR
                     YTPEGGRIRLACERRGEEVVVSVRDTGIGIGPDMLPRVFDMFSQGEATRDRSRHGLGV
                     GLSLAKGLVEMHGGAIEARSAGPQQGSEFVVRLPVLQEAVHPKGDGAAAEPATEPQGA
                     KRRLLIVDDLKDNADSLAKILGLMGHEVHVAYDGEEGIQAAERHRPEVVLLDIGMPKL
                     NGY"
BASE COUNT          512 a         1132 c         1181 g          468 t
ORIGIN      
        1 cgatcccggg gccgggatca gcgcaggagg gcgcccagtc tcagatagat cgggatgccg
       61 ggctcgcgcg gcggggagcc ggcagcctcg caggagccgc acaggacgac gtccgcggcg
      121 ggcgcattcg ggaccatgac cacatggcgg tcgggtgagg ggaccggagc gccgtggtgg
      181 cggtcggcat cctcctgccg atcggccgga gcgtccagac cgcagcagga ggggacgttg
      241 aggccgtccg aagcgcaatc ggaccccggc ttgcagcagg gcatcgaggg ggcctgaccc
      301 gtaaggcacg aggcggcggg cgccgcttgc gccagcagga ccgtcaggag aaccccgacc
      361 cacacgaaac ggctcatgac cgggcagaat atacgcgggt ggtccgattt catcaaccgg
      421 tatgaaggtt ccgaggggga tcccatgact tttctcgcct tgctggttct cgctttagcc
      481 gcggcaaccc cggctccggc tgcggttccc gttctctccg tgtccggcgc gtttttcgcc
      541 ctttccgtcc cggatgcgcg caaaagcgcc tcctggtatg gggaaaaact gggcctgacc
      601 gtcgtcatgg acccgccgaa gcacgggaaa tcgaaggtca tcgtcctcga ggggggcggg
      661 ctcatcgtcg agctcatcca ggaggacgac gcagtcccgc tcggcgccac cgcaccgggg
      721 gcccgcagcc ccatgcaggt gcatgggatc gccaaggcgg gggtgatcgt gtccgatttc
      781 gaggccacgc tggcggccct gcggcagaag aaggtggaga tcgcttacgg accctatccg
      841 gccgggaagg ggcagcgcgc gaactgcatc atccgggacg gcgacggcaa cctcatccaa
      901 ctctccggac ggtcgtaagg atcgttccag gactcctttc cggtgcgaaa agtctcgaca
      961 tccgtaccgc gataggtcca aaatccctaa tccgacggtc ggcagcgaga agaaataacg
     1021 gcgcgatccc tcccgcagcg ccggggcgag gcagcagttc cgatggcccg aagccgtgcc
     1081 ggagtctacg ggatttcgat cgccgccggc ggggcggcca tcctcctccg cttcatcctc
     1141 gatccctggc tcgggaacca gcagccctac tcgatcgtct acgccgcggt cgcactcgca
     1201 gtgtggctcg gcggctaccg gcccgcgggg gtcgccgccc tggtcgcctt cctgccggcc
     1261 acctggctct tcgtggagcc gcggcaggtc atggggcacg tggggccgac cgccgcgctg
     1321 ggattcttcc tctatctcgc cacctgcggc atcgtcatcg ccttcggtga ggcgatgcgg
     1381 cgggcgagcc agcgcgccga ggcggcccgc gaggaggccc agcggcacag ccgactgctg
     1441 caggagagcg agaatcgctt ccgcgccctc gccgacagcg cgccggtgtc gatctggatg
     1501 gacgggccca ccggtgattg catcctggtg aaccgtccgt acctcgagta cacaggtctg
     1561 cgccgcgatc aggtggaagg gccggggtgg gtggatgtgg tccacccgga cgacagcgcg
     1621 cggtacgtcg gggcgttccg cgaggcgctc gcacaccgct ccgagttcca ccaggaggtc
     1681 cgcctgcgcg ggcacgacgg gacgtaccgc tccttcgagg tgttcgcccg gccgcgcttc
     1741 gaaggagatc gattcctcgg ccacgtcggc atctgcatcg acgtcacgga acgcaaggcg
     1801 gcgcaggcga ccgccgcgat gagcgacgag cgcctggcct ccgagctcga ggcgatgagc
     1861 cgcctgcacg cgctgagcag ccgcctcatg tcggcgaccg acctgaacgt cgcgctcgag
     1921 gacgtcatgg agaacgccat ggcgacgtgc ggggccgact tcggcaacat ccaggtgatc
     1981 cagccgacct ccggcgcgct cgagatcgcc gcgcagcgcg gcttccggag cgagttcctg
     2041 gagcacttcc gcgccgttgg cgccggcgac gcctcggcgt gcgcgcgcgc cctccggcag
     2101 ggcgcggcgt gcctcgtcga ggacgtgcag ctggacccgg acttcgcccc gcaccggggc
     2161 atcgcggcgg ccgcggggtt ccgggccgtg cagtcgacgc cgctcgtgac ccacgacggc
     2221 atgatcctcg ggatgctctc cacccatttc cgcgagccgc accgcctctc ggagcgggac
     2281 cggcgcttcc tggatctcta cgcgcgcctc gccgccgatc tcatcgagcg actgcgctac
     2341 gagcgcacgc tggagcaggc ggaccggcgg aaggacgagt tcctcgcgac gctggcccac
     2401 gagctgcgca acccgctggc gccgatccgc aacgccgtgc acgtgttcaa ggcgaaaggc
     2461 cccgccgatc ccgatctcac ctggagccgg caggtgatcg agcggcagct cggacagatg
     2521 gcgcggctgc tcgacgacct gctcgacgtc agccggatca cgcgcaacac gttcgacctg
     2581 cgcagggcgt ccgtgccgct cgccccggtg ctcgcgaacg cggtcgagac cagccggccg
     2641 ctcatcgagg aggcgcggca cgagctgatc gtcgaggttc ccgcggagcc gatctatctg
     2701 gaggccgacc cggtccgcct gggacaggtc ttctccaacc tgctcaacaa cgcggcgcgc
     2761 tacacgccgg agggggggcg catccggctg gcgtgcgaac ggcgcgggga ggaggtggtg
     2821 gtgtcggtgc gcgacaccgg catcggcatc ggccccgaca tgctgccgcg ggtgttcgac
     2881 atgttctcgc agggggaggc gacgcgcgat cgctcgcggc acggcctcgg ggtcggtctg
     2941 tcgctcgcca aggggctggt ggagatgcac ggcggggcca tcgaggcgcg cagcgccggg
     3001 ccgcagcagg gaagcgagtt cgtggtgcgg cttccggtgc tgcaggaggc ggtgcacccc
     3061 aagggcgacg gcgccgccgc cgagccggcg acggagccgc agggcgcgaa gcgccgcctg
     3121 ctcatcgtcg acgacctcaa ggacaacgcc gacagtctcg ccaagatcct cggcctcatg
     3181 gggcacgagg tgcacgtcgc ctacgacggc gaggagggga tccaggcggc cgagcgccac
     3241 cggccggagg tggtgctcct cgacatcggc atgccgaagc tgaacggcta cga
//
LOCUS       DASRIQ010000122        18632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1351103, whole genome shotgun sequence.
ACCESSION   DASRIQ010000122 DASRIQ010000000
VERSION     DASRIQ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 18632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18632
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351103"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFD06_02885"
     CDS             <1..694
                     /locus_tag="VFD06_02885"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02513.1"
                     /translation="LLLAALLTPALRARANLWADSRLLWEEAARRGPERARPLINLGV
                     LAADAGDPVRAGMLFDRALALEPQNAEALYNRGRLRLDQGNAEGARDDLEASVAADPT
                     SPRARINLGIARLRLGDMAGAERDFRSALDIDPGDPRGLTNLAEVRRASGSPDEAIAL
                     YREALESDSGYAHAAGRLGVALEEKGDRRGALTAYREYLARGAATDTDRRAVEAKVRD
                     LEAALATPETIR"
     gene            710..793
                     /locus_tag="VFD06_02890"
     tRNA            710..793
                     /locus_tag="VFD06_02890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Leu,seq:caa)
     gene            complement(813..1754)
                     /locus_tag="VFD06_02895"
     CDS             complement(813..1754)
                     /locus_tag="VFD06_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZN02514.1"
                     /translation="MSTRRQTTRARSVLAAGFAGLAALALASPPASVPLSTSQAADPP
                     AVPSTGGDLRLASWNIAWLAARDREGAVPRREDDYARLRRYALRLDPDVVGLQEIEGV
                     AAARRVFAPDRYAFAFAAGGGSAQRAGFAWKRGLRVTHHADVMALALGGKSRAGADIT
                     VHAARGDLRLLSIHLKSGCPGGRLDGADTACRLLAEQGRALEAWIDARAREGGAFAVL
                     GDFNRRFSERDPFWIDLDDADPPEADLFDAGFGRRPPCWNGRYRQYIDHIVLSRTAAG
                     WLVPGSFLQQVYDLSDAPWESVLSDHCPIEVRLRTGG"
     gene            1849..2661
                     /locus_tag="VFD06_02900"
     CDS             1849..2661
                     /locus_tag="VFD06_02900"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HZN02515.1"
                     /translation="MATNLWTDVEALERLARSARAAEERRDGEDGTEAGAGVPGDAPA
                     LLPESRGPGFELAAWERPAASVSGDLLDAFPIGPGEIAFVVGDVSGKGIPAGVLGAFA
                     RSIVRHVAPLGAAPGETLERVNRILCGAHLDAIYVTLFLGVLDARTGRLRFANGGHPP
                     ALRIPRRGPVAAFGAATGPILGLLDRRRFDTGEVLLDPGDLVLVHTDGVTEARRRDGA
                     FFGRRRLARALESLERRSPGDACAGIAASLRSFERDRRHDDATVLALGFDGA"
     gene            complement(2806..4509)
                     /locus_tag="VFD06_02905"
     CDS             complement(2806..4509)
                     /locus_tag="VFD06_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN02516.1"
                     /translation="MDILERIDERDGKATSRPVLGALGLFVTAAISVALSTLLLIDSA
                     LAGERPGSRGKALGKISDEVVDQAQDAGPLDLVPVIVQTYGDPSDVDLLLAGGRGAVI
                     RARHSAIFGYSASVPAGALDALAEDPKTERISLDAPVHAQLDIAVRAARGGVALDPAS
                     DLDGRGVGIALIDTGAQLHPDLTRSRPEGLVEVEVVGHEKGYADYYGHGTHVAGILNG
                     TGLESSGPAAFRTFRGVAPGARLISLRALNPDGTGTTTDVLRAVDWVLIHGKQYNIRV
                     LNLSLGHPVYESFVTDPLCRAVRAAVAGGIVVVVAAGNLGQVGSGYGTILSPGNEPSA
                     LTVGAMDDNRTVAREDDVLARFSARGPSLIDHIVKPDLVAPGAFIVSLRAQGAWIDAT
                     HPETVLAAGAYRTDGDATRPGSYLVLSGTSLAAPMVSGAAALMLQREPGLRPSDVKAR
                     LMASASKDDHLPFETGAGYLDIEAALASTTTASSALSPRAVPGEDGTLVLEPLEGSWD
                     SSWQQGLIWGGLRRVGTLLGTENDQITASGLLWGGAGCRLSGEDAALTTSGIMWSGGG
                     N"
     gene            4871..8710
                     /locus_tag="VFD06_02910"
     CDS             4871..8710
                     /locus_tag="VFD06_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02517.1"
                     /translation="MALTDISGIVRNLTQSAEERAIARALQHFSKGQIDKAIAVLKEA
                     QQKSPDDPAVLLELGRLLAYAQKGIESAEAFRALLRRDPRALPKVEEALEELKARHAS
                     VGPQHDAIAEHHLHRDDIPRALKALERMRPEDLRAVLPRHQSKYEQVRRGAKDGKLTK
                     TILLPAYHAALIHETLKDVPRALTVYRDIARTNPEEAGRILPRLETIAARDYQNAELR
                     LEIAALLIDAGRADDAAKQFGLALETNPRSAPAIAEAVSARIDAAGEAHDLRWVLVSA
                     LLAAGDEEGGREAMRPLIAAGNRLDDIIAALQPLTQKEKSAAAHRLLAEAFLKRGQPV
                     QAIGPLLQAAEEEGLASVQVPLEALVAAHPDLPRPHQLLADLHLEAGRAAEAVASLVQ
                     AHRLAPKESSALAPRLTKALQIDATSPDAHRMLADLLAEASEHPRAVIVLRHWLGVAP
                     GEAAQVLERLSRLAAESGDPRAALGASEASILLTQHEAALALLEPVVAQHPELSAEFL
                     HALGLLAERAPGLAPRLIALFETLEPRSPLPVAVHFARGLAQFAAGMSAAAAASFREV
                     LQSAPDRVEEVRQALERFDRSDAAAAEARYLLASIYVDRRDHEAAVRELRRPGPTHAA
                     LLDRVLKRYESIVKQTPDDLPARAGLMETLLLARRHDRALEVGKETLKIRDDASTARV
                     SLLMADALVEKRDSDGAVRRFYAAYRRDPSLGPLVIERLRRLLDAEGKHPFTCLVLGK
                     VLASQGRSEEALEVLKAARAGDPALNDSVLLEIEGLVRACPADPRPGLALLALLKESG
                     QHARAVQVISSHLDSHPDSAQRIAAYLDEIVRSQPDHPLAHYELGRALMHLNARARAA
                     DRFRTAAQLDPALAPMALRRLQEILIADPTCVIAWMSSAEVLEHRGQALQAAERLAEA
                     IARAPQDAESLLGRLEQLCRSHPDEGPMQLLFADASLRLGEHDRAVRAYGDAAARDLD
                     GAAAALRGLDAILEASPRHPEARVMRARARLRLSQSDAALTDLSEAAHLAPRLLPDVL
                     TEIESLVDERPAWAEGAILRADLLVLSGRPREAESLLQQRVEKTTARPARLQMRLRLA
                     RCASERQDEAAARAHLKDAATLATDRNEFLGRVHELHLAGLRRRLAAARERLERGEGS
                     AADLESAALACLDLGRSDEANALVAAHAPAFEQEDSLRRLRAALTLFRGDFPRAADLL
                     RPLGASAPLAFGALRGGDYPLAIETLETLATRDADPRTRRLLERTYRDMVAADLFGGS
                     RRLVAETFPAFGEGAAV"
     gene            8707..9681
                     /locus_tag="VFD06_02915"
     CDS             8707..9681
                     /locus_tag="VFD06_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02518.1"
                     /translation="MTRPICPMLQALPATDAAGQPVNRECLLEPCRFYDRQQKDCGLS
                     LQAVSTARLVAQSELDGAEARAATARLAEVSGRATGLEARIAGIEIAVGDLLGRIGTL
                     IETQQKTAERLLEEMSLLQARSQKEEASVAALTERLQRSEESTRGILEALETQRRRDQ
                     ADLDARRRDEAILVNNRGVAFYYRGACEAARDAFLKALELLPDYAEAHNNLGLVYSRL
                     GQGELAVESFRRALTADPKMGEVYNNLGFLYHTTAQFDRAAQMFGLAIENAGDSSVAY
                     SNLGNTYYAMKNPEKAVEAWRHAVDLDPMNESAKRGLRMFEQDDAAAN"
     gene            9712..10053
                     /locus_tag="VFD06_02920"
     CDS             9712..10053
                     /locus_tag="VFD06_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02519.1"
                     /translation="MKRPPTEDLYERLERELRTPAERLLLDAFLPAPSSIPSALARPP
                     APEPIPGAEAGLLGADRATARDREVEEPAPRRRPRPRKSAEPQRSLEEEIADFMNQRG
                     TALAPDTDPEK"
     gene            10098..13430
                     /locus_tag="VFD06_02925"
     CDS             10098..13430
                     /locus_tag="VFD06_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZN02520.1"
                     /translation="MPASPRVEIHHDFAVLERRLEDLLRALKREDGPLAPAAVVVPTA
                     RLRAHLQERLAERCGTLLQVRFFHHDSFAAEAATDAAAAGARAAPLRPIGEAAREAIL
                     SDAAARAGGATARWLTAVPPGAGALLATMDDLREAGIDPAAARRVAGLSDEARALLAV
                     YSEYGAALQRLRAAGFSDRAGRVAAALDHLPRFARRFHLLVHYGAYELVGMNLDLMRA
                     AQASGVPAVWLAPGHPSAPAFAYARSFWREAFGVDPIPLRDDDAPAAAAARGADDRRL
                     LGASLPRLYDEEHAPGEGAEVRFFHAQGCGGELQEAALTALQDHLGTATPACRIGVLA
                     RSLEPYAGRLADLAKAHALPLTTSASAPIAIAAAAQAAVRLLRVVLLDVPAPTLIELV
                     RSGCLIPPRGTEPARVVDSWERLVHHYRVAGGLALIGRALTRWVEESPPPPARRPAPG
                     SDGEARRRRALLADAVHLGRLVAALDRESADLRGCRDWSAFAAAARSLLEARLTPFAS
                     GAPEEDPGASGVLAALDDLEALHAAGVRFESPAAALQALERGIAAARTPLASLGPDGA
                     ARDQDEGGVRVYDVMQGRGLSFDTVILLGLNAGQFPRPARPDPFLGDDDRRRLREALG
                     RPVPMKEASRLEEHLLLALALGSARRRLVVGWQRADEEGRALSPSLALREIARIARGQ
                     PDLEEIARTARRVSGDPRQAATDSIREHGLLPPEDALLAAALDAGSPQGLLGRVETLA
                     PLVPGGAADFASGLEFLAAIESPDDRGFDTLVGALRDDARSRRFSPSRLEMLGSCPQL
                     YFFRHRLLLGEWEDPEAAHALDPREIGSAVHAVLAAVYARTDALSTGGHEALAAAVRS
                     AWAEATERIAARLDPLYPGLWEPLGGAWREAIVRFVERDLPALLARGGTVECETDVDA
                     SVTLAGRAESLVLGGRFDRLLRAQNGVVVSDYKTGGDPGLFVRPLEFLKGRRLQMPLY
                     ALMAETGTVRGTGEPLGAGRADRVAVEVLGVGPRFERAPEEARAALDPGRFAQAREGI
                     LETLGVLVRLDDAGLYPLNESSDRCEWCVFRRACRRAHPATLERLAAKPELRDYALLR
                     RKSTRAPLLAQAAALAGNGEEE"
     gene            13427..16843
                     /locus_tag="VFD06_02930"
     CDS             13427..16843
                     /locus_tag="VFD06_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZN02521.1"
                     /translation="MSGADRPRSAPAGPPDQEARVRATTLFGRNLVVTAGAGTGKTAI
                     LVERTLNLVGSGTAGIEALALITFTEKAAAELRLRLGAGLDRLLKRSGGDESDLDLRR
                     DADRSWTWLRDRGDRPGAIRERVLVALAALDAASVGTIHAFCLDLLRRHPRAAGIDPR
                     AAVGEEAALERLLTETWDRFLRGPEGPAGRAGAWRAAVALAAAPEPARHLGRALARFA
                     LPAEALEPHPVPASAPLLAPVLDEAIASLRSLRNRATGVPPKMRGLLQSAERELAAVR
                     TDGIDPARARSIAEFVRAHGSLPSAGAKLAGATPDEVKDTARTALELLKALARVDDDA
                     VAAALDIARPFARQARDASLARGLVPFDAMLRLARDLLVRDGTARRRARGRFRQILVD
                     EFQDTDPLQYEVLFLLARREPAHDFERDPPDPWRADLEPGRLFIVGDPKQSIYRFRGA
                     DIAAFRRAVALIEACGGESLALTASFRSPDRLLRPINRIFGGWLGPDNGTEWIGHHAP
                     PYVRLEAAASAGPASGPARLSVWSIDAAQGAAAREGRRAEATTIAGHIRARVAAGDDR
                     PGDFAILFRAATNIELYARALRRAGVPCLVDGGADFGERPEVIDLIVWLRAVASPNDA
                     PALLAVLRSPLGAVPDAEMHRFVEAGGRLGPGDAGAGPAASPAEMEAPALERALGRLE
                     AFRRAAAGRPAEDVIRMALHDTPLLALHAAAHDGPERIARLRRAAAQARALAAEGLSL
                     PEILTQWETAFVAGEEPGPLADETVDAVRLLTVHKAKGLEFKAVFVPDLGRAEAAKRP
                     ATGGVAWTSPEGGPGTAAIALSDGRTNAARVLHDLDHARHEEAEERRIFYVACTRARE
                     ELILVNSRRDRKAPWTDRLEVLGYAPGPDGSWPAEGPLAGGEAEHSVMSAVEPASSRT
                     EPSDPVPLFRAAERHAAAARSLVSGCRPLLASPSGPDDAVTGMAGAESATPRRRSDGG
                     RAARLAGSAVHAALARCDDFRNGPALLAAALEAARHLAAAESASPAGALTLRVEGAIR
                     GILAGFSASSLPDRLASVDVLAREAPILHRDAAGQVWSGACDLLYRDEGGLVVADYKT
                     GDPDEEGPEAEARSHAAQLKIYVEAIRSAFPGENVRGEILFLRRGRAVPA"
     gene            complement(17033..18448)
                     /locus_tag="VFD06_02935"
     CDS             complement(17033..18448)
                     /locus_tag="VFD06_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZN02522.1"
                     /translation="MTTECTQESLWFSRQSGRDVVARFDGGNITSDGGGLLLGEVERR
                     TGILRQFAACFTDHREAEQIEHTVEELVRQRVYGLALGYEDLNDHDELRRDPLLATLV
                     GKADPLGRDRTRSRDRGAALAGKSTLNRLELTRPETAASSRYKKIVLDGEAVDRLLVD
                     AFVQAHHRAPKQIILDLDATDDPLHGRQEGRFFHGYYGHYCYLPLYIFCGEHLLGARL
                     RPSDIDASAGALEEVQRIVGRIRSRWPRMRIVLRADSGFAREAIMAWCEGSGVDYVFG
                     LAKNARLSRVLGRELHQVKLECERTGLPARVFKHFSYRTRKTWSRARRVVGKAEHLPD
                     KSNPRFVVTSLSCDEWDDRALYEDLYCARGDMENRIKEQQLYLFADRTSTATMRGNQT
                     RLYFSSIAYLLLEALRRLGLKGTPLARAQCHTIRLKLLKIGAQIRVTVRKVWVSLASG
                     SPSAAVFARACVNLRASPLRT"
BASE COUNT         2460 a         6968 c         6721 g         2483 t
ORIGIN      
        1 attgctgctc gccgcgctgc tgacgccggc gctgcgtgcc cgcgcgaacc tctgggccga
       61 ttcccgcctg ctctgggagg aggcggcacg ccgcgggccg gagcgggccc gcccgctcat
      121 caacctcgga gtcctcgcgg cggacgccgg ggatcccgtc cgggccggca tgctcttcga
      181 tcgggcgctg gcgctcgagc cgcaaaacgc cgaggcgctc tacaaccgcg gacggctgcg
      241 gctcgaccag gggaacgccg aaggcgcgcg cgacgatctc gaagcctcgg tcgccgccga
      301 tccgacgagt ccgcgcgcgc gcatcaatct cgggatcgcg cggctgcgtc tcggggacat
      361 ggccggagcc gagcgcgact tccggagcgc cctggacatc gatcccgggg atccgcgcgg
      421 gctcaccaac ctcgccgagg tgcggcgcgc ctcggggagc ccggacgagg cgatcgcgct
      481 gtaccgggag gcgctggagt ccgattcggg ctacgcgcac gccgccggcc ggctcggcgt
      541 ggcgctggag gaaaaggggg accggcgcgg cgcgctcacc gcctaccgcg agtacctggc
      601 gcgcggcgcc gcgaccgaca ccgatcgccg ggccgtcgag gcgaaggtgc gcgatctcga
      661 ggccgccctt gcgactccgg agacgattcg ctaggcttcg atcaccgatg ccggagtggc
      721 gaaactggta gacgcacggg actcaaaatc ccgcgggcgc aagcccatgt cggttcgagt
      781 ccgacctccg gcacggcgct ccgctcctcc tctcagcctc ctgtgcgcag cctgacctca
      841 atcggacagt ggtccgagag caccgactcc cagggggcgt cggagaggtc gtagacctgc
      901 tggaggaagg atccggggac gagccagccg gctgcggtgc ggctcaggac gatgtgatcg
      961 atgtattgac ggtaccggcc gttccagcag ggcggccggc gcccgaagcc cgcgtcgaac
     1021 agatccgcct cgggcgggtc ggcgtcatcg agatcgatcc agaacgggtc gcgctccgag
     1081 aagcggcggt tgaagtcgcc cagcaccgcg aacgcccccc cctcccgggc ccgcgcgtcg
     1141 atccacgcct cgagcgcgcg cccctgttcg gccagcagcc ggcaggctgt gtccgcgccg
     1201 tcgaggcggc cacccggaca gccgctcttg aggtggatgc tcaggagcct gagatcgccg
     1261 cgcgccgcgt gcaccgtgat gtcggccccc gcacggctct tcccaccgag ggcgagcgcc
     1321 atcacgtcgg catggtgcgt cacacggagc ccgcgcttcc acgcgaagcc ggcgcgctgg
     1381 gccgagcctc ctcccgcggc gaaggcgaaa gcgtagcggt cgggtgcgaa gacccggcgc
     1441 gccgcagcca ccccttcgat ctcctgaagc cccaccacgt cggggtccag gcgcagcgcg
     1501 taccgccgca gccgcgcata gtcgtcctcc cgccgcggca ccgccccctc gcgatcccgc
     1561 gccgcgagcc aggcgatatt ccaggacgcg agccggagat ccccgccagt cgaggggacg
     1621 gccggcggat cggcggcctg cgaggtcgac agcggaacgg aggcaggtgg cgaagcgagg
     1681 gccagcgcgg cgagccccgc gaatcccgcg gcgaggaccg aacgagcacg agtcgtttga
     1741 cgtcgcgttg acattggaga cgtcttttta cctcggacac cgaccctttg acacgatctg
     1801 tccgtaccgt ccgtggcacg cgacgggaac gcgcccgggg gaaggacgat ggccaccaac
     1861 ctgtggacgg atgtcgaagc gctcgagcgg ctcgcccgct cggcccgggc cgccgaggaa
     1921 cggcgcgacg gtgaagacgg gaccgaggcg ggagcaggag ttccgggaga cgcgccggcg
     1981 ctccttccgg aatcccgggg gccgggattc gagctcgccg cctgggaacg gccggccgct
     2041 tcggtgagcg gcgacctgct cgatgccttc ccgatcggac cgggcgagat cgccttcgtc
     2101 gtcggcgacg tctccggcaa ggggattccg gccggcgtct tgggcgcgtt cgcgcgttcg
     2161 atcgttcggc acgtcgcgcc gctcggggcc gcccccggcg agacgctcga gcgggtcaac
     2221 cgcatcctgt gcggggcgca tctcgatgcg atctacgtca cgctgttcct cggcgtgctg
     2281 gacgcgcgca ccggccggct gcgtttcgcc aacggaggcc atccgccggc actgaggatc
     2341 ccgcgccgcg ggccggtggc cgcgttcggc gcggcgaccg ggccgatcct cggcctgctc
     2401 gatcggcgcc gcttcgacac gggggaggtc ctgctggatc cgggggatct ggtcctcgtg
     2461 cacaccgacg gcgtcaccga agcccgccgc cgggacggcg ccttcttcgg ccggcgccgg
     2521 ctggcgcggg cgctcgagag tctcgagcgg cgctcccccg gcgacgcctg cgcgggcatc
     2581 gccgcctcgc ttcgttcgtt cgagcgcgat cgccggcacg acgacgcgac cgtcctggcg
     2641 ctcgggttcg acggcgcctg atcgaagtcc ccgaaaaagt cgacggccgc gccggggagg
     2701 gcggcgcggc cgtctgtcgg gtcgcggagt acgggggtca gaccccgcgg cgtctccgcg
     2761 tcgttcccgg gtggaacgcg tcctcgtagg tcatctcgtc cttcctcagt tgccgccgcc
     2821 ggaccacatg atcccgctcg tcgtgagcgc cgcgtcttcg ccgctcaggc ggcagccggc
     2881 gccgccccag aggagtcccg aggcggtgat ctggtcgttc tcggtcccga gcagggtgcc
     2941 gacgcggcgc aagccgcccc agatgagccc ctgctgccac gacgaatccc acgacccctc
     3001 gagcggctcc aggacgagcg tgccgtcctc ccccggcacc gcgcggggcg acagggcgga
     3061 cgatgccgtc gtcgtggacg cgagcgccgc ctcgatgtcg aggtatccgg cgccggtctc
     3121 gaacggcagg tgatcgtcct tggacgcgct cgccatgagg cgtgccttca cgtccgacgg
     3181 ccgaaggccg ggctcgcgct gcagcatgag ggccgccgcc ccgctgacca tcggcgccgc
     3241 gagcgaggtg ccggagagca cgaggtagct ccccggccgc gtcgcgtcgc cgtcggtgcg
     3301 gtacgcgccg gcggcgagga ccgtttccgg atgcgtcgcg tcgatccacg cgccctgggc
     3361 gcgcagcgac acgatgaacg cgcccggcgc caccaggtcc ggcttgacga tgtggtcgat
     3421 gaggctcgga ccgcgcgccg agaaacgggc gagcacgtcg tcctcgcgcg ccacggtgcg
     3481 gttgtcgtcc atcgccccca cggtgagggc gctcggctcg ttgccggggc tcaggatggt
     3541 cccgtagccg ctgccgacct gccccaggtt gccggcggcc accacgacca cgatcccgcc
     3601 ggcgaccgcg gcgcggaccg cgcggcagag cggatcggtg acgaacgact cgtacaccgg
     3661 gtggccgagg gacaggttca ggacgcggat gttgtactgc ttgccgtgga tgaggaccca
     3721 gtcgacggcg cgcaggacgt cggtggtggt gccggtgccg tccgggttca gcgcgcgcag
     3781 cgagatgagg cgcgcgccgg gggcgacgcc gcggaacgtg cggaacgccg ccggaccact
     3841 gctctccagg ccggtgccgt tcaggatgcc ggcgacgtgc gtgccgtgcc cgtagtagtc
     3901 ggcgtacccc ttctcgtggc cgacgacctc gacctccacg agcccctccg gacgggaacg
     3961 ggtcaggtcg gggtgcagct gcgcgccggt gtcgatgagg gcgatgccga cgccgcgacc
     4021 atcgaggtcc gaagcggggt cgagcgccac gccgccgcgc gccgcgcgga ccgcgatgtc
     4081 gagctgggcg tgcaccggcg cgtccaggga gatgcgttcg gtcttcgggt cctcggccag
     4141 cgcgtcgagc gcgcccgccg ggaccgacgc ggaataaccg aagatggcgg agtggcgggc
     4201 gcggatgacg gcgccgcggc cgcccgccag gagcagatcg acgtcgctcg ggtcgccgta
     4261 ggtctgaacg atgaccggca cgagatcgag cggcccggcg tcctgcgcct ggtccaccac
     4321 ttcgtccgag atcttcccga gcgccttgcc gcgcgatccc gggcgctcgc ctgcgagagc
     4381 cgagtcgatg agcagcagag tcgacagggc cacgctgatt gcggccgtga cgaagagtcc
     4441 cagcgctccc aggacgggtc gtgatgtcgc ctttccgtcg cgctcgtcga tcctctccag
     4501 gatgtccatg gcctcactct ccgcttgcgc cctgaccggt caggctcacg gcgccgaccc
     4561 tctgtagagc aagccaggga ccagccacgg gaacgtcatc ccacccgcaa acacatgtcc
     4621 tgcaatgggt tgcggtggcg cgacaggttc gtgatcagcg ccctcgggag gggtcgaatg
     4681 ggcgggatcg cgacaccgtc atgcgaaatc ggccgaatga aatccgtgac ccgctgaaga
     4741 gacagcggtt gcgagacgcg tccagacggc gacagcgcgt gtgcggagaa tggccacgtc
     4801 catcctccca gggcgggtga cgaccttgtg gccgcgacct atattgcggg cggccggggg
     4861 ccgtgcgcgc atggcgttga ccgacatctc gggaatcgtc cggaacctca cgcagagcgc
     4921 cgaggagcgc gccatcgccc gcgctctgca gcacttcagc aaaggccaga tcgacaaggc
     4981 catcgccgtg ctgaaggagg cgcagcagaa gtctcccgac gatccggcgg tgctcctgga
     5041 gctgggtcgc ctgctggcct acgctcagaa ggggatcgag tcggcggagg ccttccgcgc
     5101 gctgctgcgg cgcgatccgc gggcgctccc caaggtcgag gaagcgctcg aggaactcaa
     5161 ggcgcgccat gcctcggtcg gaccgcagca cgacgccatc gccgagcacc atctccaccg
     5221 cgacgacatc ccccgcgcgc tcaaggcgct cgagcggatg cgccccgagg acctgcgcgc
     5281 cgtcctcccc cgtcaccagt cgaagtacga gcaggtgcgg cgcggagcga aggacgggaa
     5341 gctgacgaag acgatcctcc tgcccgccta tcacgccgcc ctcatccacg agacgctcaa
     5401 ggacgtgccg cgcgccctga cggtgtaccg ggacatcgcc cgcaccaacc cggaagaggc
     5461 aggccgcatc ctgccgcgcc tcgagacgat cgcggcgcgc gactaccaga acgccgagct
     5521 gcgcctggag atcgccgcgc tgctcatcga cgcgggacgc gccgacgacg cggcgaagca
     5581 gttcggcctc gccctcgaga ccaatccccg ctcggcgccc gccatcgccg aggcggtgtc
     5641 ggcgcggatc gacgccgccg gagaggcgca cgacctgcgc tgggtcctgg tctcggcgct
     5701 cctcgcggcc ggcgacgagg agggggggcg cgaggcaatg cgaccgctca tcgcggccgg
     5761 caaccggctc gacgacatca tcgccgcgct gcagcccctg acacagaagg agaagtcggc
     5821 ggcggcccac cggctgctcg ccgaggcctt cctgaagcgc ggccaacccg tccaggcgat
     5881 cgggcccctg ctgcaggcgg cggaagagga ggggctcgcc tccgtccagg ttccgctcga
     5941 ggcgctggtc gcggcccacc cggacctgcc gcgcccccac cagctgctcg ccgacctgca
     6001 tctcgaggcg ggacgcgccg ccgaggcggt ggcttccctc gtgcaggccc atcgcctggc
     6061 gccgaaggag tcgagcgcgc tcgccccgcg gctgacgaag gcgctgcaga tcgacgccac
     6121 ctccccggac gcgcaccgca tgctggccga cctgctggcg gaggcttccg agcacccccg
     6181 cgccgtgatc gtcctgcgtc actggctggg cgtcgccccg ggggaggcgg cccaggtgct
     6241 cgagcgcctg tcccgcctcg ccgcggagag cggtgacccg cgcgccgcgc tgggcgcctc
     6301 cgaggcgtcc atcctcctga cgcagcacga ggcggcgctg gcgctgctcg aaccggtcgt
     6361 cgcgcagcac ccggagctct ccgcggagtt cctgcacgca ctcggcctcc tggcggagcg
     6421 cgccccggga ctggccccgc gtctcatcgc gctcttcgag acgctcgagc cgcgatcgcc
     6481 gcttccggtg gcggtccact tcgcgcgtgg cctggcgcag ttcgccgccg ggatgtcggc
     6541 cgccgcggcg gcctccttcc gcgaggtcct gcagtcggcc ccggaccggg tcgaggaggt
     6601 gcggcaggcc ctcgagcggt tcgatcgctc cgacgcggcg gcggccgagg cgcgctacct
     6661 gctcgcctcg atctatgtcg accgccgcga ccacgaggcg gcagtgcgcg agctgcggcg
     6721 gcccgggccg acccacgcgg cgctcctcga tcgggtgctg aagcgctacg agagcatcgt
     6781 gaagcagacg ccggacgacc tcccggcgcg cgccggcctc atggagacgc tgctcctggc
     6841 gcgccggcac gatcgcgcgc tggaggtcgg caaggagacg ctcaagatcc gcgacgacgc
     6901 ctcgaccgcg cgcgtgtcgc tcctgatggc cgacgccctg gtcgagaagc gggacagcga
     6961 tggcgccgtg cgccgcttct acgccgcgta ccggcgcgat ccgtcgctcg ggcccctcgt
     7021 gatcgagcgg ctgcggcgcc tgctcgacgc cgaggggaaa catccgttca cctgcctggt
     7081 gctcggcaag gtgctggcgt cgcagggacg gtccgaggag gcgctcgagg tcctcaaggc
     7141 ggcgcgcgcc ggcgacccgg cgctgaacga ctcggtcctc ctggagatcg aggggctcgt
     7201 gcgcgcctgc cccgccgatc cccgccccgg cctcgcgctg ctcgcgctcc tgaaggagtc
     7261 cgggcagcac gcccgggcgg tccaggtgat ctcctcgcac ctcgactcgc accccgattc
     7321 ggcgcagcgc atcgccgcct acctcgacga gattgtcagg tcgcagccgg accatcccct
     7381 ggcgcactac gagctcgggc gggcgctcat gcacctcaac gcccgggccc gcgccgccga
     7441 ccggttccgc accgccgcgc agctcgatcc ggcgctcgcg ccgatggcgc tgcggcgact
     7501 tcaggagatc ctcatcgccg atccgacctg cgtcatcgcc tggatgtcga gcgccgaggt
     7561 cctggagcac cgtggccagg cgctgcaggc ggccgagcgc ctcgccgagg cgatcgcccg
     7621 cgccccgcag gacgcggaga gcctgctcgg ccgcctcgag cagctgtgcc ggtcccaccc
     7681 ggacgaaggg ccgatgcagc tgctgttcgc cgacgccagc ctgcgcctcg gagagcacga
     7741 tcgcgccgtc cgcgcctacg gcgacgcggc ggcccgggat ctcgacggcg ccgccgccgc
     7801 gctgcgcggt ctcgacgcca tcctcgaggc cagcccgcgc cacccggagg cgcgcgtcat
     7861 gcgcgcccgc gcgcggctgc gcctgtcgca gagcgacgcc gcgctcacgg atctctccga
     7921 ggcggcgcac ctggcgccgc ggctgctccc cgacgtcctc acggagatcg agtcgctggt
     7981 ggacgagcgc ccggcgtggg ccgagggggc gatccttcgc gccgacctcc tggtcctctc
     8041 cggcaggccg cgcgaggccg aatcgctcct ccagcagcgc gtcgagaaga ccaccgcgcg
     8101 ccccgcgcgt ctgcagatgc gtctgcgcct ggcgcgctgc gcctcggagc gtcaggacga
     8161 ggccgcggcc cgcgcccacc tcaaggatgc cgcgaccctc gcgaccgacc gcaacgagtt
     8221 cctcggacgc gtgcacgagc tgcacctcgc gggcctgcga cgccggctgg cggcggcgcg
     8281 cgagcggctg gagcgcggcg agggatcggc ggccgatctc gaatccgccg cactcgcctg
     8341 cctcgatctc ggccgcagcg acgaggcgaa cgccctcgtc gcggcgcacg ccccggcctt
     8401 cgagcaggag gactccctcc ggcggctgcg cgccgccctg acgcttttcc gcggcgactt
     8461 cccgcgcgcg gccgacctgc tgcgcccgct cggcgcctcg gcgcccctgg cgttcggggc
     8521 gttgcgcggc ggcgactatc ccctggccat cgagacgctc gagacgctcg ccacgcggga
     8581 cgccgacccg cgcacgcgga gactgctcga gcggacctat cgcgacatgg tggcggccga
     8641 cctgttcggc ggcagccgcc gcctggtggc ggagactttc cccgcgttcg gcgaaggagc
     8701 ggccgtatga cccgaccgat ctgcccgatg ctgcaggcgc tcccggcgac cgacgccgcg
     8761 ggccagcccg tcaaccgcga gtgcctgctg gagccctgcc gtttctacga ccgccagcag
     8821 aaggactgcg gtctgtcgct gcaggcggtc tcgaccgcgc ggctggtcgc ccagtcggag
     8881 ctcgacggcg ccgaggcgcg cgccgcgacc gcccgcctgg cggaggtctc cgggcgggcc
     8941 acgggcctcg aggcgcggat cgcggggatc gagatcgccg tgggcgatct gctcggccgc
     9001 atcggcacgc tcatcgagac gcagcagaag acggccgagc ggctgctcga ggagatgtcg
     9061 ctgctccagg cgcgctccca gaaggaggag gcgtcggtcg ccgccctgac cgagcgcctg
     9121 cagcgatcgg aggagagcac ccgcggcatc ctcgaggcgc tggagacgca gcgccggcgc
     9181 gaccaggcgg acctcgacgc gcgccggcgg gacgaggcga tcctcgtcaa caaccgcggg
     9241 gtggccttct actaccgcgg cgcctgcgag gccgcgcgcg acgcgttcct caaggcgctg
     9301 gagctcctgc cggactacgc cgaggcgcac aacaacctcg gcctcgtcta ctcccggctc
     9361 ggacaggggg agctggcggt cgagtcgttc cgccgggcgc tgaccgcgga tccgaagatg
     9421 ggagaggtgt acaacaacct cgggttcctc tatcacacca cggcgcagtt cgatcgcgcg
     9481 gcgcagatgt tcggcctcgc catcgagaac gcgggcgact cgtcggtcgc ctactccaac
     9541 ctcggcaaca cctactacgc catgaagaat ccggagaagg cggtcgaggc ctggaggcac
     9601 gccgtcgatc tcgacccgat gaacgagagc gcgaagcgcg ggctccggat gttcgagcag
     9661 gacgatgcgg ccgccaactg atcgcacgcg ccccgggccg gggaggatcg cgtgaagcgc
     9721 ccgccgaccg aagacctcta cgagcggctg gagcgggagc ttcggactcc cgccgagcgg
     9781 ctgctgctcg acgcgttcct tcccgctccg tcgtcgatcc catccgccct ggcgcgcccg
     9841 ccggcgccgg agccgatccc cggggcggag gccggtctcc tgggcgccga tcgggcgacc
     9901 gcccgcgacc gggaggtgga ggagccggca ccacggcgcc gtccgcgccc ccggaagtcg
     9961 gcggagccgc agcgcagcct cgaggaggag atcgccgact tcatgaacca gcgcggcacc
    10021 gccctcgctc ccgacaccga cccggagaag tgacccggcg cgtctgatag gatccgcgcc
    10081 cggttccggg atcgcgggtg cctgcctctc cacgcgtcga gatccaccac gattttgccg
    10141 tcctcgagcg ccgcctcgag gacctcctgc gcgcgctcaa gcgggaggac ggcccgctcg
    10201 ccccggcggc ggtcgtcgtg ccgaccgcgc ggctgcgggc ccacctccag gagcgcctgg
    10261 cggagcgctg cggcaccctc ctccaggtcc gcttcttcca tcacgacagc ttcgcggccg
    10321 aagcggccac cgacgcggcg gcggccggcg cccgcgccgc gcccctgcgg ccgatcggcg
    10381 aggcggcccg cgaagcgatc ctctccgacg ccgccgcgcg cgccggcggg gcgacggcgc
    10441 gatggctcac ggcggtcccg ccgggggccg gggcgctcct cgccaccatg gacgacctgc
    10501 gcgaggcggg aatcgatccg gcggcggcgc ggcgcgtcgc cgggctgagc gacgaggcgc
    10561 gcgccctcct ggcggtctac tccgaatacg gcgcggcgct tcagcggttg cgcgccgcgg
    10621 gcttctccga ccgcgccggc cgcgtcgccg ccgcgctcga ccacctgccg cgcttcgccc
    10681 gtcgcttcca tctcctggtg cactacggcg cctacgagct cgtcggcatg aatctcgatc
    10741 tgatgcgcgc cgcccaagcc tccggcgtgc cggcggtctg gctcgcgccc gggcatccga
    10801 gcgcgccggc gttcgcctac gcgcgctcgt tctggcggga ggcgttcgga gtcgatccga
    10861 tcccgctccg ggacgacgac gccccggccg ccgccgcggc gcggggcgcg gacgaccggc
    10921 gcctgctcgg tgcgtccctg ccgcggcttt acgacgagga gcacgccccc ggcgagggcg
    10981 ccgaggtgcg gttcttccat gcgcagggat gcggaggcga gctgcaggag gcggcgctga
    11041 cggcgcttca ggatcacctc ggcaccgcga cgccggcgtg ccgcatcggg gtgctcgccc
    11101 gcagcctgga gccgtacgcc ggccgcctcg cggacctggc gaaggcgcac gccctgccgc
    11161 tcaccaccag cgcctcggcg ccgatcgcga tcgcggccgc ggcccaggcg gcggtccgcc
    11221 tgctgcgcgt ggtgctcctc gatgtcccgg cgccgaccct catcgagctc gtgcggagcg
    11281 gatgtctcat ccctccgcgc gggaccgagc ccgcgcgggt ggtcgattcg tgggagcggc
    11341 tggtgcacca ttaccgcgtc gcggggggac tggccctgat cggccgcgcg ctcacgcgct
    11401 gggtcgagga gtcgcccccg ccgcccgcgc gtcgtcccgc tccgggcagc gacggggaag
    11461 cgcgccgccg gcgcgcgctt ctcgccgacg cggtccacct cggccgcctc gtggcggcgc
    11521 tcgaccggga gtccgcggat ctccgcggct gtcgcgactg gagcgccttc gccgccgcgg
    11581 cccggtcgct gctggaggcg cgcctgacgc cgttcgcctc cggcgcgccg gaggaggatc
    11641 ccggcgcgtc cggcgtcctc gccgcgctcg acgacctgga agcgcttcac gcggcagggg
    11701 tccggttcga atcgccggcg gcggccctcc aggcgctcga gcgcggcatc gcggcagcgc
    11761 ggactcccct cgcgtcgctc ggccccgacg gcgcagcgcg cgatcaggac gaagggggcg
    11821 tgcgcgtcta cgacgtgatg caggggcgcg ggctgtcctt cgacaccgtc atccttctgg
    11881 gcctgaacgc cggacagttc ccgcggcccg cccgtcccga tccgttcctc ggcgacgacg
    11941 accgccgccg cctgcgagag gcgctcgggc gaccggtgcc gatgaaagaa gcgtcccggc
    12001 tcgaagagca cctccttctg gccctcgccc tcggcagcgc ccgacggcgc ctcgtcgtcg
    12061 gctggcagcg cgccgacgag gaggggcggg cgctctctcc gagcctggcg ctgcgcgaga
    12121 tcgcccgcat cgcgcggggc cagcccgacc tcgaggagat cgcccgaacc gcccgccgcg
    12181 tcagcggcga tccgcgccag gcggcgaccg actccatccg ggagcacggg ctgctgccgc
    12241 cggaggatgc gctcctcgcc gccgccctcg acgcgggatc gccgcagggg ctcctgggtc
    12301 gggtcgagac gctggcgccg ctggtccccg gcggcgccgc cgacttcgcg tccggcctgg
    12361 agtttctcgc cgccatcgaa tcgccggacg atcgcggctt cgacaccctc gtcggcgcgc
    12421 tccgggacga cgcgcggagc cgccgctttt ccccgtcccg cctggagatg ctcggctcct
    12481 gcccccagct ctacttcttc cggcatcgtc tgcttctcgg ggagtgggag gacccggagg
    12541 cggcgcacgc gctcgacccg cgcgaaatcg gcagcgccgt ccacgccgtc ctcgccgccg
    12601 tctatgcgcg gaccgacgcc ctctcgaccg gcgggcacga agcgctcgcc gccgccgtgc
    12661 gcagcgcgtg ggcggaggcg accgagcgca tcgccgcgcg cctcgacccg ctctatcccg
    12721 gcctgtggga gccgctcggc ggcgcctgga gggaggcgat cgtccgcttc gtcgagcggg
    12781 acctccccgc actgctcgca cgcggaggaa ccgtggagtg cgagaccgac gtggacgcgt
    12841 ccgtgacgct cgcgggccgg gcggagtcgc tcgtcctcgg aggccggttc gatcggctcc
    12901 tgcgcgcgca gaacggcgtc gtcgtcagcg actacaagac cggcggcgat cccggcctgt
    12961 tcgtccgccc gctggagttt ctcaagggac gccggctgca gatgccgctc tacgccctga
    13021 tggccgagac cgggacggtg cgcggcaccg gcgaaccgct cggggccggc cgtgcggatc
    13081 gcgtggcggt cgaggtcctc ggagtcgggc cgcgtttcga gcgcgcgccg gaggaggcgc
    13141 gggccgcgct cgaccccgga cgattcgcgc aggcccgcga ggggatcctc gagaccctcg
    13201 gcgtcctggt gcgtctcgac gacgccgggc tctaccccct caacgagtcg agcgaccgct
    13261 gcgagtggtg cgtcttccgc cgcgcctgcc ggcgggcgca tcccgccacg ctcgagcggc
    13321 tcgcggcgaa gccggagctg cgcgactacg cgctgctgcg gcgcaagagc acccgggcgc
    13381 cgctcctcgc ccaggccgcg gccctggccg ggaacgggga ggaggaatga gcggcgcgga
    13441 tcggccccga agcgctccgg ccggacctcc cgatcaggag gcgcgcgtcc gcgccacgac
    13501 cctcttcggg cgcaacctcg tcgtcacagc gggggccggc accggcaaga ccgccatcct
    13561 cgtggagcgg actctcaatc tcgtcggctc cgggacggcg gggatcgagg cgctggcgct
    13621 catcaccttc acggagaagg cggcggccga gctacgtctc aggctcggcg ccgggctcga
    13681 ccgactgctc aagcgatcgg gcggcgacga gtcggatctc gacctccgcc gcgacgccga
    13741 tcgttcctgg acatggctcc gcgatcgcgg cgaccgcccg ggagcgatcc gcgagcgggt
    13801 cctcgtggcc ctcgctgcgc tcgacgccgc gtcggtcggc acgatccacg ccttctgcct
    13861 cgatctgctg cgccggcacc cgcgcgccgc cgggatcgat ccacgcgccg cggtcggcga
    13921 ggaggcggcg ctcgagcggc tcctcacgga gacatgggat cgcttcctgc gcggcccgga
    13981 agggccggcg ggccgcgccg gggcctggcg cgctgcggtg gccctggccg ccgcgcccga
    14041 gccggcccgc cacctggggc gggccctggc ccgcttcgcc cttcccgccg aggcgctcga
    14101 gccgcacccg gttcccgcgt ccgcgcccct cctcgctccg gtactcgacg aggcgatcgc
    14161 ctcgctgcgg tcgctccgga acagggccac cggcgtgcca ccgaagatgc gcgggcttct
    14221 ccagagcgcc gagcgggagt tggcggcggt ccggacggac gggatcgacc cggcgcgcgc
    14281 ccgctccatc gcggagttcg tgcgggccca cggctcgctg ccgtccgcgg gggcgaagct
    14341 cgcgggggcc accccggacg aggtgaagga cacggcgcgc accgcgctcg aactcctcaa
    14401 agcgctcgca cgggtggacg acgacgcggt ggccgccgct ctcgacatcg cccgcccgtt
    14461 cgcccggcag gcgcgggacg cgtcgctggc gcgcgggctc gtcccgttcg acgcgatgct
    14521 gcggctggcg cgcgacctcc tggtgcgcga cggcacggcg cgacgacggg cgcgcggccg
    14581 cttccggcag atcctggtcg acgagttcca ggacaccgac ccgctgcagt acgaggtgct
    14641 gttcctcctc gcgcggcgcg agcccgccca cgacttcgag agggatcctc ccgatccgtg
    14701 gcgcgcggat ctcgagccgg gacgtctctt catcgtcggg gatccgaagc agtcgatcta
    14761 ccgcttccgc ggcgccgaca tcgccgcctt ccgccgcgcc gtcgccctca tcgaggcctg
    14821 cgggggggag tcgctcgcgc tgacggcttc gttccgaagc cccgatcggc tgctgcggcc
    14881 gatcaaccgg atcttcggcg gctggctcgg tccggacaac gggaccgagt ggatcggtca
    14941 tcacgcccct ccctacgtcc ggctcgaggc ggccgcatcc gccgggcccg cgagcggccc
    15001 ggcgcgcctc tcggtctggt cgatcgatgc cgcacagggg gcggccgccc gcgaagggcg
    15061 ccgggccgag gcgaccacca tcgccggcca catccgcgcg cgcgtcgccg ccggcgacga
    15121 ccgcccgggc gatttcgcga tcctcttccg ggccgccacg aacatcgagt tgtatgcccg
    15181 cgccctccgc cgggcggggg tcccgtgtct cgtggacgga ggagccgact tcggagagcg
    15241 cccggaggtg atcgacctca tcgtctggct ccgggcggtc gcgagcccca acgatgcgcc
    15301 ggcgctcctc gccgtccttc gctcgccact cggtgcggtc ccggatgcgg agatgcaccg
    15361 gttcgtcgag gctggaggcc gcctcggccc gggggacgcg ggtgcggggc cggccgcgtc
    15421 ccccgcggag atggaggcgc cggcgctcga gcgggcgctc ggacgcctcg aagctttccg
    15481 ccgcgccgcc gccggccgac ctgcggagga cgtgatccgc atggcgcttc acgacacacc
    15541 gcttctcgcc ctgcacgccg ccgcccacga cgggccggag cggatcgcgc gcctccgccg
    15601 ggccgcggcg caggcgcgcg ctctcgcggc ggaggggctg tcgctcccgg agatcctgac
    15661 tcagtgggag accgccttcg tcgccggcga ggagccgggg ccgctcgccg acgagacggt
    15721 cgacgcggtg cggctgctca ccgtccacaa ggcaaagggg ctcgaattca aggcggtctt
    15781 cgtccccgac ctgggacggg cggaggccgc gaagcggccc gcgacggggg gggtcgcctg
    15841 gacctcgccg gagggagggc ccgggaccgc ggcgatcgcc ctctcggacg gccgcacgaa
    15901 tgcggcgcgc gtccttcacg acctcgacca tgcgcggcac gaggaggcgg aggagcgccg
    15961 catcttctac gtcgcctgca cccgcgcccg cgaggagctg atcctggtga acagccgacg
    16021 ggaccggaag gccccctgga ccgatcgtct ggaagtcctc ggctacgcgc ccgggccgga
    16081 cggctcctgg ccggccgagg ggccgctcgc gggaggcgag gcggagcaca gcgtgatgag
    16141 cgcggtggag ccggcttcct cccggacgga gccgtccgat cccgttcccc tcttccgggc
    16201 ggcggagcgg catgcagcgg ccgcgcggag cctcgtgtcc ggatgccgtc ccctcctcgc
    16261 ctccccttcc ggcccggacg acgccgtcac cgggatggcc ggggcggagt ccgcgacgcc
    16321 gcggcgccgg tccgatggag gacgtgcggc gcgtctcgcc ggctcggccg ttcacgcggc
    16381 gctcgcccgc tgcgacgact ttcggaacgg tccggccctc ctcgccgcgg cgctcgaagc
    16441 cgcccggcac ctcgcggccg cggagtccgc gagcccggcc ggcgctctca ccctgagggt
    16501 cgaaggtgcg atccgcggca tcctggccgg gttctccgcc tcgtccctgc cggatcggct
    16561 ggcgtcggtc gacgtgctcg cgcgggaggc gccgatcctc caccgggatg ccgcaggcca
    16621 ggtctggagc ggcgcctgcg atctcctgta tcgggacgag ggggggctcg tcgtcgccga
    16681 ctacaagacc ggcgacccgg acgaggaggg acctgaggca gaggcgcggt ctcacgccgc
    16741 gcagctgaag atctacgtcg aggcgatccg ctccgccttc ccgggggaga acgtgcgcgg
    16801 cgaaatcctg ttcctgcgcc gaggccgggc ggtcccggcc tagcccgcat ttctcaccag
    16861 tgatcgccag gcgcccctaa tcgggggaga ggcgggcggt ctgagaagcg cgatcacgga
    16921 cgggaagtgt tccgggtcgc tgaccgtcgc ggcaaggttc cttatgcgat ctcggcggat
    16981 cgctgagacg cacttcgact ggcgcgatgc gtcgaagagg tcgatgggcc ggtcaggtgc
    17041 gcagcgggct cgcgcgcagg ttgacgcagg cgcgtgcgaa cacggctgca gacggtgagc
    17101 cgctggcgag cgacacccac actttgcgca ccgtgactcg gatctgggct ccgatcttca
    17161 ggagcttgag tcggatcgtg tgacactgcg cccgagcgag aggcgttccc ttcaagccca
    17221 gacgccgaag ggcctccagg agcagatagg cgatcgagga gaagtacagc ctggtctggt
    17281 tgccccgcat agtcgcggtg ctggtgcggt cggcgaacag gtagagctgc tgctccttga
    17341 tgcggttctc catatcgccg cgggcgcagt acagatcctc gtacaacgcg cggtcgtccc
    17401 actcgtcgca cgataacgag gtcaccacga agcgcgggtt ggacttgtcg ggaaggtgct
    17461 ctgctttgcc gaccacgcgc cgcgcgcggc tccaggtctt gcgggtccgg tagctgaagt
    17521 gcttgaagac ccgcgccggc aggccggtgc gctcgcactc cagcttcacc tggtgcagct
    17581 cgcgccccag cacccggctc agtcgggcgt tcttcgccag gccgaagaca tagtccacgc
    17641 cggaaccttc gcaccaggcc atgatcgctt cacgcgcgaa gcccgagtcg gcgcgcagca
    17701 cgatgcgcat cctcggccag cgcgatcgga tccgccccac gatgcgctgc acctcctcga
    17761 gggcgcccgc cgaggcatcg atgtccgacg gtcgcaaccg ggcgcccagc aagtgctcac
    17821 cgcagaagat gtacagcggc aggtagcagt agtggccgta gtagccgtgg aagaagcgcc
    17881 cttcctgccg cccgtgcagc gggtcgtcgg tggcgtccag atcgaggatg atctgcttcg
    17941 gggcccggtg atgcgcctgc acgaaggcat ccaccagcag ccggtcgacc gcctcaccgt
    18001 ccaagacgat cttcttgtag cggctgctcg cagcggtctc cggccgcgtc agctccagcc
    18061 ggttcagcgt gctcttgccg gccagcgccg cgccgcggtc acgcgagcgt gtccggtccc
    18121 gccccagagg atccgccttc ccgaccagag tcgccagcag cgggtcccgc cgcagctcat
    18181 cgtggtcgtt gaggtcttcg taacccagcg ccagcccgta cactcgctgc ctgaccagct
    18241 cttcgaccgt gtgctcgatc tgctctgctt cgcgatggtc cgtgaagcag gccgcgaact
    18301 gtcgcaggat cccggtccgc cgctccacct caccgagcag cagcccgccc ccgtccgacg
    18361 tgatgttccc gccgtcgaac cgagccacaa cgtcgcgtcc actttgtcgg ctgaaccaga
    18421 gcgactcttg ggtacactct gtcgtcatag ggctgttctc ctatccaggt cttaaagaag
    18481 cgtcgcagct gctttatgcc tgaaagggaa cagccctttc aacgtctatg gtgagaaatc
    18541 cgggctagag aggctgccgt tccgatcgcc gaggagcccg aggaggaagc gggcggccgc
    18601 caggcgcggc accgatcccg ccggaacgaa gc
//
LOCUS       DASRIQ010000123         8761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3447628, whole genome shotgun sequence.
ACCESSION   DASRIQ010000123 DASRIQ010000000
VERSION     DASRIQ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3447628"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFD06_02940"
     CDS             <1..809
                     /locus_tag="VFD06_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN02523.1"
                     /translation="RTPAGAQIPLSQIADLRMVQGPSMLRNENGLLSGYVYVDTAESD
                     IGGFVDRAKAAVAGAVTLQPGYSLEWSGQYENMLRVRERLKLVVPVTLVLITLLLYAN
                     TGSMARAGIVLLAVPFSVVGAVWLMWALDYNVSIAAWVGMIALMGLDAETGVFMLLYL
                     DLAYKDAVAAGRMRNREDLQEAIIHGAVRRIRPKMMTVSAAFAGLMPIMWSLGTGADM
                     MKRVAAPMVGGLVTSFLLELLVYPPLYELWKWYAEVRRQSQTPMETRLTG"
     gene            complement(734..1453)
                     /locus_tag="VFD06_02945"
     CDS             complement(734..1453)
                     /locus_tag="VFD06_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02524.1"
                     /translation="MEDDRTFFRVGGVCAALSGVTTFLLWLLPRTYDAPAGFEESIAL
                     HAHAGYMARWWVNFVHIFLALAGYAATAWALRARSRMLSALGFLWFLLWGFTELLGVS
                     IHIWAVNRTWRAGWAAADAATRDILRANLTGFSAVWDGMFFLLLVAFLLGTLCFGIAA
                     VSGGSGIAAARGSGLERWVGAMLLLAVPLTIAILLDGYAGMTAAGRAVERIYPVLQPV
                     SRVSMGVWLWRRTSAYHFQSS"
     gene            complement(1454..1969)
                     /locus_tag="VFD06_02950"
     CDS             complement(1454..1969)
                     /locus_tag="VFD06_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN02525.1"
                     /translation="MAAGPAGAAAPDGKAPSKASPEARIKELGITLPPAPKPMASYVP
                     VVRTGNLLFLAGQGPTAEGKVLVAGKVGSDLTEEEGNKAARASILNSLAILKSELGSL
                     DRVRRIVKMTGWVNSAPGFNRQPWVINGASDLLIEIFGEAGKHARSAVGTNELPLNIP
                     VEIEIVVEAAP"
     gene            2229..2684
                     /locus_tag="VFD06_02955"
     CDS             2229..2684
                     /locus_tag="VFD06_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02526.1"
                     /translation="MRVAIVIVMVAGVVACREQPKADVHAVEVRMVAHWPTWHATYLI
                     EEPQGPVTEVPTGREVHVPLGAEVTLALSSRDYISDFSLPGLGLRDFAAPGLPSEFRF
                     RAERPGRFTLRGDELCGRPHDERSRGWLVVEDPASFRAWIGRRARAGLG"
     gene            2769..5375
                     /locus_tag="VFD06_02960"
     CDS             2769..5375
                     /locus_tag="VFD06_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZN02527.1"
                     /translation="MTPEQRFRRVAWWVVMGGMLLVALIVVLLREYNWLSASDVFDLE
                     ADATSDDGAAAPAGGPGLRVLATLPEFVLENERGDSFGLLDLRGRVWVADFIFTRCAG
                     TCPMITERMADLAAAIDRDPSLAEIRLVSISVDPDFDRPEVLRDYARARRAEVPRWTF
                     LTGTRAAVRGLVKDGFKLPVEDQNDPAMPILHSQAFVLVDRAGRLRTIADPLAEGGVD
                     GVLRALAGLVAEPKGNDLAFPHDAADPRWYQHRERAQRLTERSIGVRHDFRFKDRLGA
                     SGIRFRHAASPDLTRFYRASHYDHGTGIAAADVDADGRPDLYFVNQAGRNALYRNLGG
                     GRFEDVTDSASLTGTAGTGVGLGVGDRACVGAAFADVDNDGDPDLFVTAIRAGNLLFR
                     NDGHGRFADVTQEAGVAGPGGHSSGAVFFDYDGDGRLDLFVTNVGVYTGDERREDGLW
                     VSFPDAFAGHLHPDRSETSLLYRNRGDVRFEEASRSSGLVHAGWSGDATPYDDNGDGR
                     PDLYVGSMQGHDELWRNVGGGRFERRGRQVFPATPWGTMGVVVLDWNGDGRFDLFATD
                     MHTDMASDLRPENERRKHDWSTMYPPRFLGTDGNHILGNALFTNQGGDRFTEMSDAAN
                     AETGWPWGPSAGDLNADGWMDLFVAAGMSFPFRYRGNDLLLNDAGRRFANAEFLLGVE
                     PRTRLLRPWFDLACDGADRSHPICKGEELTVMSLDGSSKVEGGPRTPLHGDVTLWASR
                     SGRSAVLLDLDDDGDLDIVVNDYGDVPQILVSDLAQRGEVNFLRVRLKGARSNRDALG
                     AVVTVRAGGRARHQVHDGKSGYLGQSALPLYFGLGTATQADSVSVRWPGGTTQSMRGP
                     FRSGSTVSLEER"
     gene            complement(5350..>8761)
                     /locus_tag="VFD06_02965"
     CDS             complement(5350..>8761)
                     /locus_tag="VFD06_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02528.1"
                     /translation="ISEKVVGERRVLALRDLGGRLVEAMTVEEACVFAAETLAAYDKD
                     VPFVFFYLPNEDATEARLAAAAGLGNAEAIAPPMIDLREPRPGWPLFMARMSGAVQLV
                     EDLAGKFPEVPAGPWSDPPHRAVVLPIPSSKPQEPAAFMVAGLSSRLRWDPFYRDFFD
                     LARTRVATAIANARAYEEEKRRAEGLAELDRAKTAFFSNVSHEFRTPLTLMLAPIEDL
                     LERSRSGPEAALHGDLEVVHRNGQRLLRLVNTLLDFSRMEAGRTRARYQPTDLAAFTA
                     DLASVFRAAVERAGLKLVVECHAPSEPVYVDREMWEKIVLNLLSNAFKFTFHGEIAVT
                     LKERSDRQVELLVRDTGTGIPAGEMPRLFERFHRVHNARGRTHEGSGIGLALVQDLVR
                     QHGGRIGATSRPGEGTTFSVVVPLGSAHLPPEQIAEGQAAAVSGKATTAFVEEAMRWF
                     PDAVVEGQADAQGEVLDDEPAPAAEAGTESAEADDARPLVLIADDNADMRQYLSRLLA
                     GRYGTEVAADGEMALAAVRRRAPDLILTDVMMPRLDGFGLLQRLRQEPDTRHIPVIML
                     SARAGEESRVVGMEAGVDDYLVKPFSARELLARVSGHLQMARLRREASEAMEESHDRF
                     EALFNAAPIGVYLVDADLRIIQVNPKALPMFGDIENLIGSDFSEVIHVLWPKPYADEI
                     VARFRHTLATGQPYFVPERVEERLDRRVQEYYEWQIHRIALPDGRHGVVCYFSDISRH
                     VMARQALAEADRRKNEFLAVLAHELRNPLAPLRSALQVMKLAQDDPATVKDSRLMMER
                     QLTLLVRLIDDLMDMSRISRGKVELRRERIALSDAVRLAVETSRPEIEARGHEFVVEL
                     PSDRIGVDADVDRLAQVFSNLLNNAAKYTDPGGHITLSVQRHGAEAVVRVRDTGMGIP
                     PEMLPKVFDIFTQVDRTIEAAQGGLGIGLSLVKGLVEMHGGTVEARSEGRGKGSEFVV
                     QLPVRSLEAEKQRSDWGGATTRIPSRRVLIVDDNRDAAVSLAAMLNFMGNETQTAHDG
                     YEALDVAASFRPDLVMLDIGMPRLSGYDTARRIREQPWGRGMVLVALTGWGQEEDRRK
                     SKDAGFDSHLVKPIEPGAMERLLAGLPRPPGPHSAAATAPFNAPRATR"
BASE COUNT         1276 a         3187 c         2972 g         1326 t
ORIGIN      
        1 tccggacgcc ggccggcgct cagatcccct tgagccagat tgccgacctg cgcatggttc
       61 aagggccctc catgttgagg aacgagaacg gtctgctctc gggctacgtc tacgtggaca
      121 cggcggagag cgacatcggc ggattcgtcg atcgggcgaa agcggcggtg gcgggagccg
      181 tcaccttgca gcccggctac tcgctcgaat ggagcgggca gtacgagaac atgctgcggg
      241 tgcgcgagcg cctgaagctc gtcgtgcccg tgacgctcgt cctgatcacg ctcctgctct
      301 atgcgaacac gggctcgatg gcgcgcgcgg ggatcgtcct gctggccgtg cccttctccg
      361 tcgtcggcgc ggtctggctg atgtgggcgc tcgactacaa cgtgtcgatc gcggcatggg
      421 tcggaatgat cgccctcatg ggcctggacg ccgagaccgg cgtcttcatg ctgctgtacc
      481 tcgacctcgc ctataaggac gcggtcgccg caggccgcat gcgcaaccgc gaggacctgc
      541 aggaggcgat catccacggc gccgtccgcc gcatccgtcc caagatgatg accgtgtccg
      601 ccgccttcgc cgggctgatg ccgatcatgt ggtcgctcgg caccggcgcc gacatgatga
      661 agcgcgtcgc cgccccgatg gtcggcgggc tggtgacctc cttcctcctc gagctgctcg
      721 tctatccgcc gctctacgag ctctggaagt ggtacgccga ggtgcgccgc cagagccaga
      781 cgcccatgga gacgcggctg accggctgaa ggacggggta gatcctttcc acggcccggc
      841 ccgccgccgt catgccggcg tacccgtcca gcaggatggc gatcgtcagc ggcacggcca
      901 ggagcagcat cgcgccgacc caccgctcga gcccgctccc ccgcgccgcg gcgattcccg
      961 agccgccgga gacggcggcg ataccaaagc agagcgtccc caagaggaaa gccacgagga
     1021 gcaggaagaa catcccgtcc cacacggcgg agaagccggt gaggttggcg cgaaggatgt
     1081 cgcgggtcgc cgcgtccgcc gccgcccagc cggcccgcca cgtccggttc accgcccaga
     1141 tgtggatcga gacgccgagc agctcggtga acccccagag caggaaccag aggaacccga
     1201 gcgcggagag catccggctc cgcgcccgca gcgcccacgc cgtcgccgcg tagcccgcca
     1261 gcgccaggaa gatgtggacg aagttgaccc accagcgcgc catgtagccg gcgtgcgcgt
     1321 gcagggcgat gctctcctcg aacccggccg gtgcatcgta ggtccggggc aggagccaga
     1381 gcaggaacgt cgtgaccccc gacagcgcgg cgcacacgcc gccgaccctg aagaaggtcc
     1441 ggtcgtcttc catctagggc gccgcctcga cgacgatctc gatctcgacc gggatgttga
     1501 gcggcaattc gttcgtcccg acggcgctcc gggcgtgctt cccggcttca ccgaagatct
     1561 cgatgagcag atcggacgcg ccgttgatca cccacggctg tcggttgaat cccggcgcgc
     1621 tgttgaccca accggtcatc ttgacgatgc gccgcacccg gtcgagcgaa cccagctccg
     1681 acttcaggat ggcgagcgag ttcaggatgc tcgcgcgcgc cgccttgttc ccctcctcct
     1741 cggtgagatc gcttccgacc tttcccgcga ccagcacctt cccctcggcc gtcggcccct
     1801 gcccggcgag gaacagcaga ttcccggtcc gcaccaccgg cacgtaggaa gccatcggct
     1861 tcggagccgg cggcaacgtg attcccagct ccttgatccg cgcctcaggc gatgccttcg
     1921 agggcgcctt tccatcggga gccgctgcgc cggccgggcc cgccgccatc gcggttgcca
     1981 tcgcgaccat cgcgaaccca agcatgcgtc tcttccaccg catgatgact ttcatcgagg
     2041 tcctcctgtc atagatctca ctgaatctct ccacgctgaa ccggtgaagt gcgttgacaa
     2101 tttttttacg aacgggtgac ggaagtgtcc cacgcgtccc ggaatcggga atcgccgtgc
     2161 gccaaaggat ttccggcaac tcccccacgc ggggatgcgt agtgccttcg gggcgaaagc
     2221 gagtcgtcgt gcgcgtagca atcgtcatcg tgatggtcgc gggagtcgtg gcctgccggg
     2281 aacagccgaa ggcggacgtc cacgccgtcg aagtgcgcat ggttgcacac tggccgacct
     2341 ggcacgccac ctacctcatc gaagaaccgc aggggcccgt caccgaagtg ccgaccgggc
     2401 gcgaggtgca cgtcccgctc ggcgccgagg tgacgctggc cctctcgagc cgcgactaca
     2461 tcagcgactt ttccctaccg gggctcgggc tgcgcgattt cgcggccccg ggtctcccct
     2521 ccgagttccg cttccgggcc gaacgtcccg gccgcttcac tctgagggga gacgaattgt
     2581 gcggccggcc gcacgacgag cgatcgcgcg gctggctggt ggtggaagat ccggcgtcat
     2641 tccgggcctg gatcggcaga cgggcgcggg cggggttggg atgaggtgtc cgcagtcacg
     2701 ggcgggcggc ctcccctggg caggggcggg actcggatga accgcgcgcg ggcatgggta
     2761 tgcccgggat gacgccggag caacgcttcc gacgagtcgc ctggtgggtg gtgatggggg
     2821 gaatgctcct cgtcgccctc atcgtggtgc tcctgcgtga gtacaactgg ctgagcgcct
     2881 cggacgtctt cgacctcgag gcggacgcga cctccgatga cggcgccgcc gccccggcgg
     2941 gcggccccgg cctacgcgtc ctcgcgaccc ttcccgagtt cgtcctcgag aacgagcgcg
     3001 gagactcgtt cggccttctc gacctgcgcg ggcgcgtctg ggtcgccgac ttcatcttca
     3061 cccgctgcgc cgggacctgc ccgatgatca ccgagcggat ggcggacctc gccgccgcca
     3121 tcgaccgcga cccgtcgctc gccgagatcc gtctggtgtc gatcagcgtc gaccccgact
     3181 tcgaccggcc ggaggtgctg cgcgactacg cccgcgcccg gcgggccgag gtgccgcgct
     3241 ggacgttcct caccggcacg cgcgccgcgg tgcgcggcct cgtgaaggat ggattcaagc
     3301 tgccggtcga ggatcagaac gacccggcga tgccgatcct ccacagccag gcgttcgtgc
     3361 tcgtcgatcg cgccggccgc ctccggacca tcgccgaccc gctggccgag gggggcgtgg
     3421 acggcgttct acgcgccctc gcggggctcg tcgccgagcc gaaggggaat gacctcgcct
     3481 tcccgcacga cgccgccgat ccgcgctggt accagcaccg cgagcgcgcc cagagactga
     3541 ccgagcgctc gatcggcgtg cggcacgact tccgtttcaa ggaccgcctc ggcgccagcg
     3601 gcatccgctt ccgccacgcc gccagccccg acctcacgag gttctaccgc gcgtcgcact
     3661 acgaccacgg caccgggatc gcggcggccg acgtggacgc cgacggccgt cccgacctct
     3721 atttcgtcaa ccaggccggc cgcaacgccc tctaccgcaa tctcggcggc ggccgcttcg
     3781 aggacgtcac ggacagtgcg agcctgaccg gtaccgccgg gacgggcgtc ggcctcggcg
     3841 tcggcgaccg ggcgtgcgtc ggggcggcgt tcgccgacgt ggacaacgac ggcgatccgg
     3901 acctcttcgt caccgccatc cgggcgggga acctcctgtt ccgcaacgac ggccacggac
     3961 gcttcgccga cgtcacccag gaggccggcg tcgccggacc gggaggccat tcctcggggg
     4021 ccgtgttctt cgactacgac ggcgacggcc ggctcgacct gttcgtcacg aacgtcggcg
     4081 tctacaccgg cgacgagcgc cgcgaggacg ggttgtgggt ctccttcccg gacgccttcg
     4141 ccgggcacct ccaccccgat cgctccgaaa cctccctgct ctaccggaac cgcggcgacg
     4201 tccggttcga ggaggcgtcc cgatcctcag gtctcgtgca cgccggctgg tcgggggacg
     4261 cgacgccgta cgacgacaac ggcgacggcc gccccgatct gtacgtcggc tcgatgcagg
     4321 ggcacgacga gctgtggcgc aatgtcgggg gaggccgctt cgagcggcgc ggccggcagg
     4381 tgtttccggc gacgccgtgg ggcaccatgg gggtcgtcgt cctggactgg aacggagacg
     4441 gccgcttcga cctgttcgcc accgacatgc acaccgacat ggcgagcgac ctccgcccgg
     4501 agaacgagcg acgcaagcac gactggagca ccatgtaccc gccccggttc ctcggcaccg
     4561 acgggaacca catcctcggc aacgccctgt tcacgaacca ggggggggat cgcttcaccg
     4621 agatgtcgga cgccgccaac gccgagaccg gctggccgtg gggaccgagc gccggcgacc
     4681 tgaacgccga cggctggatg gatctcttcg tcgccgccgg catgtcgttc cccttccgct
     4741 accgcggcaa cgacctcctg ctgaacgacg cgggacgccg tttcgcgaac gccgagttcc
     4801 tcctgggggt cgagccgcgc acccggctgc tccgcccctg gttcgacctc gcctgcgatg
     4861 gcgccgaccg gtcgcacccg atctgcaaag gcgaggagtt gacggtcatg tctctcgacg
     4921 gctcctccaa ggtggagggg ggcccgagga cgcccctgca cggtgacgtg accctctggg
     4981 cgtcccgctc cgggcgctcc gccgtcctgc tcgatctcga cgacgacggc gacctcgaca
     5041 tcgtcgtcaa cgactacggc gacgtccccc agatcctcgt gagcgatctg gcgcaacgcg
     5101 gcgaggtgaa tttccttcgc gtgcgcctga aaggcgcccg ctccaaccgc gacgccctcg
     5161 gcgccgtcgt cacggttcgc gccggcggcc gcgcccggca ccaggtccac gacggcaagt
     5221 ccggctatct cggccagagc gcgctgccgc tctacttcgg gctggggacg gcgacgcagg
     5281 cggactccgt gagcgtccgg tggcctggag gaacgacgca gtcgatgcgc ggaccgttcc
     5341 gctcgggctc taccgtgtcg ctcgaggagc gttgaaaggc gcggtcgccg ccgcgctgtg
     5401 cggccccgga ggacgcggca ggcccgcgag cagcctctcc atcgccccgg gctcgatcgg
     5461 cttcaccagg tgcgagtcga aacctgcgtc cttcgacttg cgccggtcct cctcctgccc
     5521 ccagccggtc agcgccacca gcaccatccc tcgtccccag ggctgctcgc ggatgcgccg
     5581 ggcggtgtcg tagccggaga gacgcggcat gccgatgtcc agcatgacga ggtcgggccg
     5641 gaaggaggcg gctacgtcga gcgcctcgta gccatcgtgg gcggtctgcg tctcgttccc
     5701 catgaagttc agcatcgcgg cgaggctgac cgcggcgtca cggttgtcgt ccacgatgag
     5761 cacgcgccgg gacgggatcc gcgtcgtggc gccgccccag tcgctgcgct gcttctctgc
     5821 ctcgagcgag cggaccggca gctgcacgac gaactcgctc cccttcccgc gcccttcgct
     5881 cctcgcctcc accgtccccc cgtgcatctc gacgagcccc ttcacgagcg acaacccgat
     5941 gccgagcccc ccctgcgccg cttcgatggt gcggtccacc tgcgtgaaga tgtcgaacac
     6001 cttggggagc atctcggggg ggatccccat gccggtgtcg cgcacccgca ccaccgcctc
     6061 cgctccgtgc cgctgcaccg agagcgtgat gtggccgccc gggtcggtgt acttggcggc
     6121 gttgttcagg aggttcgaga agacctgcgc caggcggtcc acgtcggcgt ccacgccgat
     6181 ccggtcggac ggcagctcga cgacgaactc gtggccgcgc gcctcgatct ccgggcggct
     6241 ggtttcgacc gcgagccgca ccgcgtcgga gagcgcgatc cgctcccgcc gcagctccac
     6301 cttgccgcgg ctgatccggc tcatgtccat gaggtcgtcg atcagccgga cgaggagcgt
     6361 cagctgccgc tccatcatga ggcgggagtc cttcacggtc gccgggtcgt cctgcgcgag
     6421 cttcatgacc tggagcgcgc tcctcagcgg cgcgagcgga ttgcgcagct cgtgcgccag
     6481 gaccgccagg aactcgttct tgcgccggtc ggcctccgcc agcgcctgcc gcgccatcac
     6541 gtgccgggag atgtcgctga agtagcagac gaccccgtgc cgtccgtccg gcagcgcgat
     6601 ccggtggatc tgccactcgt agtactcctg gaccctgcgg tccaggcgct cttccacccg
     6661 ctccggcacg aagtacggct ggccggtggc gagcgtgtgg cggaaacgcg cgacgatctc
     6721 gtcggcgtac ggcttcggcc agaggacgtg gatcacctcg gagaagtccg agccgatgag
     6781 gttctcgatg tcgccgaaca tcggcagcgc cttcgggttc acctggatga tgcgcagatc
     6841 cgcgtccacg aggtagacgc cgatcggcgc ggcgttgaac agcgcctcga agcggtcgtg
     6901 gctctcctcc atcgcctcgc tcgcttcgcg cctcaggcgc gccatctgga ggtggcccga
     6961 gaccctcgcc aggagctcgc gggcgctgaa cggcttgacg aggtagtcgt ccacccccgc
     7021 ctccatgccg acgacgcggc tctcctcgcc ggcgcgcgcc gacagcatga tcaccgggat
     7081 gtggcgcgtg tccggctcct gccgcagacg ctgcagaaga ccgaacccgt ccagccgcgg
     7141 catcatcacg tcggtgagga tgaggtcggg ggcccggcgg cggaccgccg ccagcgccat
     7201 ctcgccgtcg gcggcgacct ccgtcccgta gcgaccggcg aggaggcgcg acaggtactg
     7261 ccgcatgtcg gcgttgtcgt cggcgatgag gacgagcgga cgcgcgtcgt ccgcctccgc
     7321 ggactccgtg ccggcctcgg cggccggcgc cggctcgtcg tcgaggacct ccccctgggc
     7381 gtcggcctgc ccttcgacga ccgcgtcggg gaaccagcgc atcgcctcct cgacgaaggc
     7441 ggtcgtcgct ttgccggaga cggccgccgc ctgcccttcg gcgatctgct ccggcggcag
     7501 gtgcgcggac ccgagcggca cgacgacgct gaaggtcgtc ccctcccccg gccggctcgt
     7561 ggcgccgatg cgtccgccgt gctgccgtac gaggtcctgc accagcgcca ggccgatgcc
     7621 gctcccctcg tgcgtccggc cgcgcgcatt gtggacccgg tggaagcgct cgaagaggcg
     7681 tggcatctcg ccggcgggga tgccggtgcc ggtgtcccgc accaggagct cgacctgccg
     7741 atcggaccgc tccttgagcg tcacggcgat ctcgccgtgg aaggtgaact tgaaggcgtt
     7801 ggagagcagg ttgaggacga tcttctccca catctcccga tcgacgtaga ccggctcgga
     7861 cggtgcgtgg cactcgacga cgagcttcag gcccgcccgc tcgacggcgg cgcggaacac
     7921 gctcgcgaga tccgcggtga aggcggcgag gtcggtcggc tggtagcggg cccgcgtgcg
     7981 gcccgcctcc atgcgggaga aatcgaggag ggtgttgacc aggcgcagca gccgctgacc
     8041 gttgcggtgc acgacctcga gatcgccgtg cagcgccgcc tccggtcccg agcggctgcg
     8101 ctcgaggagg tcctcgatcg gcgccagcat cagcgtcagg ggcgtgcgga actcgtggct
     8161 gacgttcgag aagaacgccg tcttcgcccg atccagctcc gccagcccct cggcgcggcg
     8221 cttctcctcc tcgtaggcgc gcgcgttggc gatcgcggtc gccacccggg tgcgggcgag
     8281 atcgaagaag tcgcggtaga aggggtccca gcgcagccgc gacgaaaggc cggcgaccat
     8341 gaaggcggcg ggttcctgag gcttgctcga cgggatcggc aggaccaccg cccggtgcgg
     8401 cgggtcggac cacggccccg ccggcacctc cggaaacttc cccgccaggt cctcgaccag
     8461 ctggaccgcg ccgctcatcc gggccatgaa caggggccag ccgggccggg gctcgcgcag
     8521 atcgatcatc ggaggcgcga tcgcctccgc attccccaac cccgccgcgg cggcgagccg
     8581 cgcttcggtc gcgtcctcgt tcggcagata gaagaagacg aacggcacgt ccttgtcgta
     8641 cgccgcgagc gtctcggcag cgaagacgca cgcctcctcg accgtcatcg cctccaccag
     8701 ccgcccgccc agatcgcgca gcgccagcac ccggcgctcg ccgaccacct tctcgctgat
     8761 c
//
LOCUS       DASRIQ010000124         3678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3448943, whole genome shotgun sequence.
ACCESSION   DASRIQ010000124 DASRIQ010000000
VERSION     DASRIQ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3448943"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VFD06_02970"
     CDS             complement(<1..1211)
                     /locus_tag="VFD06_02970"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN02529.1"
                     /translation="MRRVWPGLFVILLSILDAFAQSAPKYKDLKPIPDTPALRRAQEV
                     LEVINLGDPARIRALVESAFAPAFRDGASMDDHFAAFAGWHDMNQRLEPYGARTYDPP
                     EPETHAVIVARSRLLETWRAVVVDVEPQAPHRITSVDVARARPPLDQPAAPKLGPAEI
                     AKTLGAFVDRLAAAGLFSGTVLVRQNDTILVTRAVGVANRDFDAKVRLDTKFNLGSMN
                     KMFTGVAAMKLVQQGKLSLDDPIGKYLDATWLPKVDRNKVLVKHLLTHTSGLGSYFNE
                     TFDRSSRLLYRKVDDYKPLVAEETLAFEPGTKWAYSNTGMLIAGAVIEKASGMDYFEF
                     VRSSVTGPAGMKNTDCYELDRVNPNLAVGYERRSTPGGVEYRNNVFEHVLRGGPAGGG
                     YSTVEDLDRFS"
     gene            1344..2222
                     /locus_tag="VFD06_02975"
     CDS             1344..2222
                     /locus_tag="VFD06_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02530.1"
                     /translation="MRPGSPAQAARAAVSAVAVLACLSSVAALGAGSSYELDTSLSVL
                     IDTHRDPDAKVFVDERGHMVLLIPFQPTLFLVDRSAGTAFALRKEHAVRSKDGATLKV
                     RRELFAWSVPVSLEGSSPRFRIGASEVRVIEQARGEPDAEASAPPATPPIAPAPDPPA
                     ESSPGRPPQPDGPATPVNEPAGSAPAAGGPPATECVSLQTRPATGVPGCTRLVSIRNI
                     CEVPVVVLVQRTEHLMSGTLPQAFDTVVPKGEQFLGCAWWSGAMAPAKHEILGASYLT
                     PPGKVAHGRHGTPPRR"
     gene            complement(2238..2654)
                     /locus_tag="VFD06_02980"
     CDS             complement(2238..2654)
                     /locus_tag="VFD06_02980"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZN02531.1"
                     /translation="MPDLPLTGGCLCGQVRFEVTRPLVMAGYCHCTRCQRRTGTAAAV
                     SARIAPGSLRILSGEELVKAFRPPDGFAKIFCTNCGGALWSQSQKDPSIMSVRLGAFD
                     RDPGLRPSYRQYVAYAAKWEPIPDDGLPRFPEARTA"
     gene            complement(2787..3632)
                     /locus_tag="VFD06_02985"
     CDS             complement(2787..3632)
                     /locus_tag="VFD06_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HZN02532.1"
                     /translation="MPLSSAGSIRLATVFSLLVATLAFLPAAFGQTCTPQVPCADPRG
                     CPDLSIDVGILDQRNSTVLQTHTFAPTDCAVVEGMAVAGTRRLLLFSTQTNNRGPGAV
                     VLGNPAEHPEWFELAACHGHYHIKDYADYRLWTPAGYAQWTALRAADPGLCASQVLDA
                     NPSLQSQLVRGNKLGICFFDVLMMGQIRGSAELCPGAFDARTYVECDFAGLGVCWADI
                     YEAGLFGSIDGQWIDVTGLADGDYVLENESNANRLITEADYSNNSSAIRIRIKGKNLR
                     ILGTV"
BASE COUNT          573 a         1266 c         1252 g          587 t
ORIGIN      
        1 tgggagaagc ggtcgagatc ctcgacggtc gagtagccgc cgccggccgg cccgccgcgg
       61 aggacgtgct cgaagacgtt gttgcggtac tcgacgcctc ccggagtcga acgtcgctcg
      121 taccccaccg ccagattcgg attcacccgg tcgagctcgt agcagtcggt gttcttcatt
      181 ccggcggggc cggtgacgct cgatcggacg aactcgaagt agtccatccc cgaggccttc
      241 tcgatgaccg cgccggcgat gagcattccg gtattgctgt acgcccactt cgtccccggc
      301 tcgaaggcga gggtctcttc cgccaccagc ggcttgtagt cgtccacctt gcggtagagg
      361 agccgtgagg agcgatcgaa cgtctcgttg aaataggacc cgaggccgct cgtgtgcgtg
      421 agcaggtgct tcaccaggac cttgttcctg tccaccttgg ggagccaggt cgcgtcgagg
      481 tacttgccga tcggatcgtc gagcgacagc ttcccctgct gcaccagttt catggcggcg
      541 accccggtga acatcttgtt catggagccg aggttgaact tggtgtcgag tctgaccttg
      601 gcgtcgaagt cgcggttggc gacaccgacc gcccgggtca cgaggatcgt gtcgttctgc
      661 cgcacgagca cggtgccgct gaacaatccg gccgccgcca ggcgatcgac gaaggcgccc
      721 aacgtcttcg cgatctcggc cggtccgagt ttcggcgccg ccggctgatc gagcggcggg
      781 cgcgcccggg ccacgtccac cgaggtgatc cggtgcggcg cctgcggctc cacgtcgacg
      841 accaccgccc tccacgtctc caggagccgg ctgcgcgcca cgatcaccgc gtgcgtctcc
      901 ggctcgggcg ggtcgtaggt acgcgctccg tagggctcga gccgctggtt catgtcgtgc
      961 cagcctgcga acgctgcgaa gtgatcgtcc atcgaagcgc cgtcgcggaa ggcgggggcg
     1021 aacgccgact cgaccagcgc acggatgcgc gccgggtcac cgaggttgat gacctcgaga
     1081 acctcctgcg cccggcgcag cgccggcgtg tccgggatcg gcttgagatc cttgtacttc
     1141 ggcgcggact gtgcgaacgc gtcgagaatc gagagcagga tgacgaagag tccgggccag
     1201 acacggcgca tcaagtcctc ctggaggcgc atcagcgttc gtccccggtt ctgcgcgggg
     1261 cggcagcatg aacggccggc gggcgcccgg ggttccgcgc ctcgtccgcc ccccgggcac
     1321 gaacgctggt atgctttgac gacatgaggc ccggttcccc tgcgcaggcc gcccgcgcgg
     1381 cagtgtccgc ggtggcggtg ctggcctgcc tctcctccgt tgcggcgctc ggcgccggat
     1441 cgtcctacga gctcgacacg tccctctccg tcctcatcga cacacaccgc gacccggacg
     1501 cgaaggtctt cgtggacgag cgcggtcaca tggtgctcct gattccgttc cagcccacgc
     1561 tgtttctcgt cgatcggtcc gccgggaccg ccttcgctct caggaaggag cacgcggtgc
     1621 gctcgaagga cggagccacg ttgaaggtgc ggcgggagct gttcgcgtgg agcgtcccgg
     1681 tttcgctgga aggctcctcg ccgcggttcc ggatcggcgc gtccgaagtg cgcgtcatcg
     1741 agcaggcgcg cggcgagccc gacgcggagg catctgctcc gccggcgact cccccgattg
     1801 ccccggcgcc cgatccgccg gccgaatcat ccccgggaag gccgccacag ccggatggac
     1861 ccgccacacc cgtcaacgag cccgccggat ccgcgccggc ggcggggggt ccgccggcga
     1921 cggagtgcgt ctcgctccag acgcggcccg cgaccggggt ccctggctgc accaggctcg
     1981 tgtccatccg gaacatctgc gaggtgccgg tggtcgtcct ggtgcagagg accgagcacc
     2041 tgatgagcgg cacgcttcct caggccttcg acacggtcgt cccgaaggga gagcaattcc
     2101 tcggctgcgc ctggtggagc ggcgcgatgg ccccggcgaa gcacgaaatt ctcggggctt
     2161 cctacctcac gccgcccggg aaggtcgccc acggccggca cggaacgcct ccccgccgct
     2221 gaccaatcgc caggagccta ggcggtccgc gcctccggga accgcggcag gccgtcgtcc
     2281 gggatcggct cccacttcgc cgcgtaggcg acgtactgcc ggtacgacgg gcggagaccc
     2341 gggtcccgat cgaacgcccc caaccgcacg ctcatgatgg atgggtcctt ctgactctgg
     2401 ctccacagcg ccccgccgca gttcgtgcag aagatcttcg cgaagccgtc cggcggacgg
     2461 aacgccttga ccagctcctc tccggacaga atgcgcagcg agccgggagc gatgcgcgcc
     2521 gacaccgccg cggccgttcc ggtccgccgc tggcaccgcg tgcagtggca gtagccggcc
     2581 atgacgagag gtcgtgtcac ttcgaagcgg acctggccac acaggcagcc gccggtcagc
     2641 gggagatcgg gcatcgtcaa tcctccttga ttcagccgcg cgggttcagg cgggacccag
     2701 catcgtcaac cacgagggaa atggtcgtca agcggatcgt tccgacggaa cgattcggcg
     2761 tggagcacct cctggaattc tcgagctcag accgtcccga ggatgcgcag gttctttccc
     2821 ttgatccgga tccggatggc ggaggagttg ttcgagtagt cggcctcggt gatgaggcga
     2881 ttggcgttcg actcgttctc gagcacgtag tccccgtcgg cgaggccggt gacgtcgatc
     2941 cactgcccgt cgatgctgcc gaagagcccc gcttcgtaga tgtcggccca gcacacaccc
     3001 agccccgcga aatcgcattc gacgtacgtg cgcgcgtcga acgcgccggg gcagagttcc
     3061 gccgagcccc ggatctgccc catcatcagc acgtcgaaga aacagatccc cagcttgttc
     3121 ccgcgcacga gctgcgactg cagggaggga ttcgcgtcca ggacctgcga cgcgcacagc
     3181 cccgggtcgg ccgcccgcag ggccgtccac tgcgcgtagc cggccggcgt ccagaggcga
     3241 tagtcggcgt agtccttgat gtggtaatga ccatgacagg cggcgagctc gaaccactcc
     3301 gggtgctctg cggggttccc gaggacgacc gcgccgggcc cccggttgtt ggtctgggtc
     3361 gagaagagga gcagccggcg cgtgccggcc accgccatcc cctccaccac cgcgcaatcg
     3421 gtcggcgcga acgtatgcgt ctgcaggacg gtcgagttgc gctggtccag gatccccaca
     3481 tcgatggaca gatcggggca ccctctgggg tcggcgcagg ggacctgagg cgtgcaggtc
     3541 tggccgaagg cggccggcag gaacgccagc gtcgccacga gcaaggagaa gacggtcgca
     3601 agacggatcg atccggcgga agaaaggggc atggaacacc tcccggcccg acacggcctt
     3661 tgatgctttg tcgtaccg
//
LOCUS       DASRIQ010000125         4901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3449936, whole genome shotgun sequence.
ACCESSION   DASRIQ010000125 DASRIQ010000000
VERSION     DASRIQ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449936"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFD06_02990"
     CDS             <1..543
                     /locus_tag="VFD06_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN02533.1"
                     /translation="KMNPPLRSEDDRLALLHAIEDGTVDVIATDHAPHHSDEKLVEFD
                     DAPFGIVGLETAVALVMDRLVRPGHIDLSRMVELMALNPARILGLKKGTLQPGADADV
                     TVIDPEATRAVDPDRFESRSRNTPFGGWKLRGWPVMTFVGGRVVHDADARAGRTGRAA
                     AGRLSRNGGRPAQPGRRAPR"
     gene            563..2476
                     /locus_tag="VFD06_02995"
     CDS             563..2476
                     /locus_tag="VFD06_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN02534.1"
                     /translation="MTTAPLRRGFVAAAAAAFLAAACGTPPDPNRPDIVLVVVDTLRA
                     DRLGVYGHHSTSPRIDQMAKQGIVFDAAWAAAPWTLPSIMSIVTGRTPSSHKVENDGL
                     RLASDVPTLPRILEERGYATGGFVSHIYVTAPFGFDRGFERFEDFGLTKPGYRPEARL
                     EPNAEKVTGAALDWLDRQGGRPVFMMVHYFDPHWPYEPPEKVRALFPTTYAGPLDAGW
                     DSLSRFQDPAVPLPEDYRAFIEKRYDGEIFFVDQQIARLIDGLEKSGRSKRSWVFVTA
                     DHGEEFKDHGSIGHGRQMYEEVVRVPLVVAGPPFVATATSKAGRGSDSARATRIATPV
                     SGIDLLPTLVEIAGASVPAGVEGRSLLPLMRAAAPQLIGSTPPAAGAIPPEIDRIPTG
                     DRPVISETVRLNANRRAVRIGDLKVIHAMDENRSELYDLAADPREAHDVAAARPDERR
                     RLTRILFARPDLLSGGWNVRWRSDGRGHRFDGRITTTGIFRSVVPLFHEGGAYRLERP
                     DTLTFADLDQKGEGGIVFTVAPETATVTFDLRVDRRPRPEWVLVGGDGTPAAQIPLAL
                     DGAAGGAAVFAPPPGALAASTRGPAGTGRPGGGGAPPAGYLLWRTRPAAPDQPVTLDE
                     ETRARLRALGYID"
     gene            2544..4595
                     /locus_tag="VFD06_03000"
     CDS             2544..4595
                     /locus_tag="VFD06_03000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN02535.1"
                     /translation="MARLGVPLAAVVGFAAQFLVSLRAEKPWATIFPGILLYLVALAL
                     LRIGLRTSGAATADADRWLRGKAEWWFLAILLLGALWLRVYRIDVVPWGLNNDEAINA
                     IEANEIAVGKEFATVTERGLNRETMFHMLAAVSYQHPEFGLNILRALPALFGLNPRTI
                     NDPLADALFPLRAVSIGAALLTLVALYFFVRASFGTRVAIVATFFLAVSPWHLLYSRV
                     GERTILAPLFMLLTAGLFLRALRTGRVVDHVLWGTALGLGFWTYTTFRAVPIALAVFC
                     VWHVRRRGTGLNLKGLLAGAGLAAAGFLFVMMFSGLSPLGFLLRGAYAATPPKTNWFL
                     NLFHAVTMPLYYPSDYAVIQSEAFISDGISPTYGLIGLEPDTLLLAAFSTLGLALVAS
                     MAFARRRDGWAAPGATEGPDAAQRSDAAALVVFLLLATWFTIGWLGPSLTRLLMIVPC
                     LVLCAAIFTVRAWDGIASLRPPITAWLGATATAGLLALATAQGFSNLFLLAGRSERAM
                     QHFGAAQTIMGMFVRSLPPEQNVIVLHTLRVDTLKYLIGARPRTRMLTDTRSVSLDDI
                     MKTPNTVTFVVEYARPFAEPLRGLMTRFPQGDMSQVADARIDPDKPIFFTFTLWKDAL
                     GNVIAPADGGIGAVPPGGEGGMPGGGAPGQGMSLPDPAFPGAENRRPPPAGQAPPPPP
                     N"
BASE COUNT          674 a         1774 c         1702 g          751 t
ORIGIN      
        1 aagatgaacc cgccgctccg ctcggaggac gaccgactgg cgctcctgca cgcgatcgag
       61 gacggcacgg tggacgtcat cgccaccgat cacgccccgc accactccga cgagaagctg
      121 gtggagttcg acgacgcccc gttcggcatc gtcgggctgg agacggcggt ggcgctggtg
      181 atggatcggc tggtgcggcc ggggcacatc gacctctccc ggatggtcga gctgatggcg
      241 ctcaacccgg cccgcatcct cggcctgaag aaggggacgc tgcagccggg ggccgacgcc
      301 gacgtgaccg tcatcgaccc ggaggcgacg cgcgccgtcg atccggaccg cttcgagtcg
      361 cgctcgcgca acaccccgtt cgggggatgg aagctgcggg gctggccggt gatgaccttt
      421 gtcggcggac gcgtggtgca cgatgccgac gcccgcgcgg gacggacggg gcgggcggcc
      481 gccggccgcc tgtcgcggaa cggcgggcgc ccggcgcagc cgggtcgtcg cgccccccgc
      541 tgagcgcgac gtcgcagccg gaatgaccac cgcccccctg cgccgcgggt tcgtcgcggc
      601 cgcggctgcg gccttcctcg cggcggcctg cgggactcca cccgatccga atcgccccga
      661 catcgtcctc gtcgtggtgg acacgctgcg ggccgaccgg ctcggcgtgt acgggcacca
      721 ttccacctcg ccgcgcatcg accagatggc gaagcagggg atcgtgttcg acgcggcgtg
      781 ggccgcggcg ccgtggaccc ttccctcgat catgtcgatc gtcacgggcc gcaccccctc
      841 gtcgcacaag gtggagaacg acgggctgcg gctggcgtcg gacgtgccga cgctcccccg
      901 gattctcgag gagcgcggct acgccaccgg cgggttcgtg tcgcacatct acgtgacggc
      961 gcccttcggg ttcgatcgcg gcttcgagcg gttcgaggac ttcgggctga cgaagcccgg
     1021 ctaccggccg gaggcgcggc tcgagccgaa cgccgagaag gtcaccggcg ccgcgctcga
     1081 ctggctcgac cggcagggag ggcggccggt gttcatgatg gtgcactact tcgacccgca
     1141 ctggccgtac gagccgccgg agaaggtccg cgcgctcttc ccgacgacct acgcgggacc
     1201 gctcgacgcc ggctgggaca gcctgtcccg cttccaggac ccggccgtgc cactgcccga
     1261 ggattaccgg gccttcatcg agaagcgcta cgacggcgag atcttcttcg tcgatcagca
     1321 gatcgcccgt ctcatcgacg gtctggagaa atccgggcga tcgaagcgct cctgggtctt
     1381 cgtgaccgcg gaccacggcg aggagttcaa ggatcacggc tcgatcggcc acggccggca
     1441 gatgtacgag gaggtggtgc gggtgccgct ggtggtcgcc ggcccgccgt tcgtcgcgac
     1501 ggcgacgtcg aaggccggcc gggggtccga cagcgcgcgc gccacccgca tcgcgacccc
     1561 ggtcagcggg atcgatctcc tcccgacgct cgtggagatc gccggcgcgt cggttccggc
     1621 cggtgtggag gggcggagcc tcctgccgct gatgcgggcg gcggccccgc agctcatcgg
     1681 ctcgacgcct ccggccgccg gggcgatccc tcccgaaatc gaccggattc cgacaggaga
     1741 ccggcccgtc atcagcgaga cggtgcggct caacgccaac cgccgcgcgg tccggattgg
     1801 agacctgaag gtcatccacg ccatggacga gaaccgctcc gagctgtacg acctcgccgc
     1861 cgatccccgg gaggcgcacg acgtcgccgc ggcacgccct gacgaacgga ggcggttgac
     1921 gcgcatcctg ttcgcgcgcc ccgatcttct gtccggcggc tggaacgtcc gctggcggag
     1981 cgacggccgc ggacaccgct tcgacggccg gatcaccacg accggcatct tccggagcgt
     2041 cgtgccgctc ttccacgagg ggggcgccta ccggctggag cgtcccgaca ctttgacctt
     2101 cgccgatctc gaccagaagg gagaaggggg gatcgtcttc accgtcgccc cggagacggc
     2161 gaccgtgacg ttcgatctcc gggtggatcg gcggccccga cctgagtggg tgctcgtggg
     2221 cggcgacggg actccggccg cgcagatccc cctggccctc gacggcgccg cgggcggcgc
     2281 ggcggtcttc gctccccccc ctggagcgtt ggccgcttcg actcgaggcc ccgccggaac
     2341 ggggcgcccc gggggcggcg gcgcacctcc ggccgggtac ctgctctggc gcacgcgacc
     2401 ggccgctccc gaccaaccgg tcacgctcga cgaggagacg cgcgcgcgtc tacgcgccct
     2461 cgggtacatc gactaaactc tccgacccca tggcgcgagg cgaagggaaa gaccgaggag
     2521 cgggaccggg aatccccccc atcgtggcga ggctcggcgt gccgctggcc gcggtcgtcg
     2581 gcttcgccgc ccagttcctg gtgtcgctgc gcgccgaaaa gccctgggcg acgatcttcc
     2641 ccggcatcct gctctatctg gttgcgctgg cactcctgcg gatcggcctg cgcaccagcg
     2701 gcgcggccac ggccgacgcc gaccggtggc tgcgcggcaa ggcggagtgg tggttccttg
     2761 cgatcctcct cctcggcgcc ctgtggctgc gcgtgtaccg catcgacgtc gtgccgtggg
     2821 ggctgaacaa cgacgaggcg atcaacgcca tcgaggcgaa cgagatcgcc gtggggaagg
     2881 agttcgcgac ggtcaccgag cgcgggctca atcgcgagac gatgttccac atgctggcgg
     2941 ccgtctcgta tcagcacccc gagttcggtc tcaacatcct gcgcgcgctg ccggcgctct
     3001 tcggtctcaa cccgcgcacc atcaacgacc cactggccga cgccctcttt ccgctgcgcg
     3061 ccgtatcgat cggggccgcg ctgctgaccc tcgtcgcgct ctacttcttc gtgagggcct
     3121 ctttcggaac gcgcgtcgcc atcgtcgcga ccttcttcct ggccgtgtcg ccgtggcacc
     3181 tcctgtacag ccgggtgggg gagcgcacga tcctggcgcc gctcttcatg ctgctcaccg
     3241 ccggcctgtt cctgcgggcg ctcaggaccg gtcgcgtggt cgatcacgtc ctgtggggga
     3301 cggcgctcgg gctcgggttc tggacctaca cgacgttccg cgccgtgccg atcgccctcg
     3361 cggtcttctg cgtctggcac gtgcggcggc gcgggaccgg gttgaatctc aagggactcc
     3421 tcgccggtgc ggggctggcg gcggccggct ttctgttcgt catgatgttc tccgggctgt
     3481 cgccgctcgg gttcctgctg cgcggcgcct acgctgcgac gccgccgaag accaactggt
     3541 tcctgaacct gttccacgcc gtcacgatgc cgctctacta cccgtccgac tacgcggtca
     3601 tccagagcga ggcgttcatc agcgacggca tcagcccgac ctacgggctc atcggcctcg
     3661 agccggacac gctgctcctc gccgccttct cgacgctcgg gctggcgctc gtggcgtcga
     3721 tggcgttcgc gcgacgccgc gacggctggg cggcgcccgg ggccacggag gggccggacg
     3781 cggcgcagcg gagcgatgcc gccgcgctcg tcgtttttct acttctcgca acgtggttca
     3841 cgatcggctg gctcgggccg agtctgacgc gccttctcat gatcgtgccg tgcctcgtgc
     3901 tctgcgccgc gatcttcacg gtacgggcgt gggacggcat cgcgtcgctg cggccgccga
     3961 tcaccgcgtg gctgggcgcg acggcgaccg cggggctcct ggcgctcgcc acggcgcagg
     4021 gattcagcaa cctgttcctg ctcgccggcc gctccgagcg ggcgatgcag cacttcggtg
     4081 cggcgcagac gatcatgggg atgttcgtgc gctcgctgcc gccggagcag aacgtcatcg
     4141 tcctgcacac gctgcgcgtg gacacgctca aatacctcat cggcgcgcgc ccgcgcaccc
     4201 gcatgctgac cgacacccgg agcgtgagtc tcgacgacat catgaagacg ccgaacacgg
     4261 tgaccttcgt cgtcgagtac gcgcgtccct tcgccgagcc gctgcgaggg ctcatgaccc
     4321 gcttcccgca gggggacatg tcgcaggtcg ccgacgcccg catcgatccc gacaagccga
     4381 tcttcttcac cttcaccctg tggaaggacg cgctggggaa cgtcatcgcc cctgctgacg
     4441 gcggcatcgg cgcggtgccg ccgggcggag agggcgggat gcctggtgga ggcgcgcccg
     4501 gccagggaat gagtctcccg gatcccgctt tccctggcgc ggaaaaccgc cgtccgccgc
     4561 ccgccggcca agcgccgcca ccgccgccga actgaacgcg gacctcgtcc agcgcagccg
     4621 cgcggcttcg ctgtgacgga agtcacaccc agaccgtgac cctcttgcgg gatcgccggg
     4681 aatcgtagaa agtcttcccc ctcgaaacaa acatttccct gggggatcac catgcgatat
     4741 gcggcggcgg gatccgatcg aacgatggga aggatgctgg ccgcgagcct gctcgccgcc
     4801 gtggcgctca caacggccgc gcaggcgcag acgcgcaccg ccgagtcctc gcgatcgcag
     4861 aagcagccca acatcctggt catcttcggc gacgacatcg g
//
LOCUS       DASRIQ010000126         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_312766, whole genome shotgun sequence.
ACCESSION   DASRIQ010000126 DASRIQ010000000
VERSION     DASRIQ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312766"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2393
                     /locus_tag="VFD06_03005"
     CDS             <1..2393
                     /locus_tag="VFD06_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN02536.1"
                     /translation="IIPHPFTPELVGPLFVRAQSAGSDAFLASHWPAPPLGNLLDDDL
                     DKNILISPAYPPFGPAYSPLDTCPHLRNGINDDFNHDGIGLRCDPGEQGDPATPNRWS
                     EVPGGSLPGARAGAASVFDAGRGVIVLFGGAADSDTWEFDGSWQRRTTSAAPEPRRGH
                     RMVYDAARARVVLYGGERADGTPLGDQWEYNGATGAWTRITTAVSPGTRSGFGLAYDA
                     GRQEIVLHGGRRGAALLGDTWVYKGGVWRLVLLSRTPGARADAAMTWDGFRKLTVLVG
                     GMGAGNSVRNDAWEFDGVSWEPVDALGELPPTTAAVASFDAARRQILVFGGFMQTRGS
                     LLTMVSQYFETSAATRSFDGRRFAPLPSLDTTEPRSSPATAFDAARDRLVVQGGAGEL
                     GSIFQGGLPQPGAPLADTALLEQATDSDGDGAADADDDCPRVADPDQEDADADGSGDA
                     CDDCPLLANADQRDRDGDGDGDACDPDRDGDGIVNAVDVCPDAFVPGRPDNAILGGGG
                     ADTDGDGLADDCDLCPADAHNDQDGDGLCGDRDNCPSTANPMQADSNGDGAGDACQPM
                     VRIVSIGPASTPPQTLEARVVLGDPDGDKPSGRIEISPATAIPEVVTSQIDPCANAFL
                     PDGNAGEGVVYVAMPSTAPFLADVDSGVGCNDGLIDYVLTYGTCSEALPGVGDATLLL
                     DRPTPFPICVRRPDGSDAHDYVVQRIRQDAVLISGALPPIVSLEYKKGRLPHQTSLQP
                     LGSNGAFILRITAGDGTTPEVHDERIFDWNGEKTMVFSQNGKKTLARPSIQRPKVF"
     gene            complement(2372..>2594)
                     /locus_tag="VFD06_03010"
     CDS             complement(2372..>2594)
                     /locus_tag="VFD06_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HZN02537.1"
                     /translation="VLPTFGGHRGHPPLFHGSLFPELLGVSEETEGLRRVVRRIPERV
                     LEVPWQRSGILLNLNTPGEYESTQKTLGL"
BASE COUNT          393 a          944 c          901 g          356 t
ORIGIN      
        1 ccatcattcc gcacccgttc actcccgagc tcgtcggccc gctgttcgtc cgggctcaat
       61 cggccggcag cgatgcgttc ctcgcctcgc actggccggc gccgccgctc gggaatctcc
      121 tcgacgacga cctcgacaag aacattttga tctcgcccgc ctacccgccc ttcggccccg
      181 cctactcacc gctcgatacc tgcccgcacc tgcgcaacgg catcaacgac gacttcaacc
      241 acgacggcat cggcctgcgg tgcgatcccg gtgagcaggg cgatccggcc acgccgaacc
      301 gctggagcga ggtcccggga ggatccctcc ccggcgcacg ggccggggcc gcctcggtct
      361 tcgacgccgg gcgcggcgtc atcgtgctgt tcggaggcgc ggccgactcc gacacctggg
      421 agttcgacgg atcctggcag cggcgcacga cgagcgccgc tccggagccg cgccgcggac
      481 accggatggt ctacgacgcg gcgcgcgccc gcgtggtgct gtacggaggc gaacgggccg
      541 acgggacgcc cctcggggat cagtgggagt acaacggcgc gaccggagcc tggacccgca
      601 tcacgacggc ggtgtccccc ggcacccggt cgggcttcgg cctggcgtac gacgccggcc
      661 ggcaggagat cgtcctgcac ggcggccggc gcggggcggc gctcctcgga gacacctggg
      721 tctacaaagg gggcgtctgg cggctcgtgc tcctgtcacg cacacccggg gcgcgggccg
      781 acgcggccat gacctgggat ggattccgga agctcaccgt cctggtgggc ggcatggggg
      841 ccgggaacag cgtccgcaac gacgcgtggg agttcgacgg cgtgtcgtgg gagccggtgg
      901 acgcgctcgg cgagctcccg ccgacgaccg ccgcggtcgc gtcgttcgac gccgcacgac
      961 ggcagatcct cgtgttcgga ggcttcatgc agacgcgcgg gagcctcctg accatggtgt
     1021 cgcagtactt cgagacgtcg gccgccaccc gatccttcga cggccggcgc ttcgctcccc
     1081 tgccgtctct cgacacgacc gagccgcgct cctcgccggc gacggcgttc gacgcggcgc
     1141 gcgatcgcct ggtcgtgcag ggaggagccg gcgagctggg cagcatcttc cagggcggcc
     1201 tgccccagcc gggcgctcct ctcgccgaca cggcgctcct ggagcaggcg accgattccg
     1261 acggggacgg tgcggcggat gccgatgacg actgcccgcg cgtcgccgac ccggatcagg
     1321 aagacgccga cgccgatggc agcggcgacg cctgcgacga ctgtccgctc ctcgcgaacg
     1381 ccgatcagcg cgatcgcgac ggggacgggg acggggatgc ctgcgacccg gaccgcgacg
     1441 gagacggaat cgtcaacgcg gtcgacgtct gccccgacgc cttcgtgccg gggcggcccg
     1501 acaacgcgat tctcgggggc ggcggtgccg acaccgacgg tgacggcctc gccgacgact
     1561 gcgacctctg cccggccgat gcgcacaacg atcaggacgg cgacggcttg tgcggagatc
     1621 gggacaactg tccctccacg gccaatccga tgcaggccga ctccaacggc gacggcgccg
     1681 gagatgcctg ccagccgatg gtgcggatcg tctcgatcgg gccggcctcg acgccgccgc
     1741 agacgctgga ggcgcgcgtc gtcctcggcg atccggacgg cgacaagcca tcgggccgga
     1801 tcgagatctc cccggcaacc gccatcccgg aggtcgtcac ctcccagatc gatccctgcg
     1861 cgaacgcctt cctgccggac ggaaacgccg gggagggagt ggtctacgtc gccatgccgt
     1921 cgacggcacc gttcctggcg gacgtcgatt cgggagtcgg gtgcaacgac ggcctgatcg
     1981 actacgtcct gacctacggg acctgcagcg aggcgctgcc gggcgtcggg gacgccaccc
     2041 ttctgctcga tcgtccgacc ccgttcccga tctgcgtgcg gcgtcccgac ggatcggacg
     2101 cgcacgacta tgtcgtgcag cggatccggc aggacgccgt gctgatctcg ggcgccctcc
     2161 cccccatcgt gtcgctcgag tacaagaaag ggcggctgcc gcaccagacg tcgcttcagc
     2221 cgctcggttc caacggagcc ttcatcctgc gcatcaccgc cggggacggc acgacccccg
     2281 aggtccacga cgaacggatt ttcgactgga acggcgagaa gacgatggtc ttcagccaga
     2341 acggcaagaa aaccctggcc cggccgtcga ttcagagacc caaagtcttc tgagtcgact
     2401 cgtactcccc tggtgtgttg aggttcagga ggatcccgga gcgctgccac ggcacctcga
     2461 ggacgcgctc cgggatccgc cgcaccacgc gacgcagccc ctcggtctcc tcgctgacgc
     2521 ccagcagctc cgggaagagc gagccgtgga agagcggcgg atggccgcgg tggccgccga
     2581 aggtcggcaa gacg
//
LOCUS       DASRIQ010000127         7497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3449984, whole genome shotgun sequence.
ACCESSION   DASRIQ010000127 DASRIQ010000000
VERSION     DASRIQ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449984"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="nfi"
                     /locus_tag="VFD06_03015"
     CDS             <1..693
                     /gene="nfi"
                     /locus_tag="VFD06_03015"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866233.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HZN02538.1"
                     /translation="RWPRTPRAAIALQKSLRPRLSLLGGPRSPRTVAGADLAYDLERD
                     RLHAVVVVFRFPSLEWVETSAVTRRIPFPYIPGLLSFREAPAIIAAWRRLRRPADLLL
                     CDGQGIAHPRGLGLASHLGLLLDVPTIGCAKSLLVGEHGPVGYDRSATTPLRHAGRIV
                     GAVVRTRAGVAPIVVSPGHRIGVARAVRLALACTRGFRIPEPTRQADLLVGRLRRGLE
                     APRPPRGHGSLC"
     gene            866..1480
                     /locus_tag="VFD06_03020"
     CDS             866..1480
                     /locus_tag="VFD06_03020"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZN02539.1"
                     /translation="MVSEQIEARGIRAPEVLAALRAVPRHLFVPAEVRESAYNDGPLP
                     IGHGQTISQPYVVAFMTEAARIKPGDRVLEIGTGSGYQAAVLDAIGARVWSIEIVPDL
                     AERARRALAATGASRVTVRAGDGTGGWPEEAPFDAIVVTAAPEQVPQALLDQLKVGGR
                     LVIPVGSGSQELLRITRTGTGFEREPLLSVRFVPMTGEARRPRP"
     gene            1533..2963
                     /gene="proS"
                     /locus_tag="VFD06_03025"
     CDS             1533..2963
                     /gene="proS"
                     /locus_tag="VFD06_03025"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZN02540.1"
                     /translation="MSKVITPRAEDYSRWYTDVVLQSQMADYSPVKGCMVIRPLGYAV
                     WEAMQRELDARFRATGHQNAYFPLLIPESFMKREAEHVEGFAPECAVVTHGGGKKLEE
                     PLIIRPTSETIIYEMYSKWIRSYRDLPLLINQWANIVRWEMRTRLFLRTTEFLWQEGH
                     TAHATEAEAEEEVLKILEIYRSFAEDCMAVPVYTGRKTNSEKFAGALRTYCIEALMQD
                     GKALQAGTSHSLGQNFAKAFDVKFQTTAGDWQHVWNTSWGVSTRLVGAVVMSHGDDNG
                     VAFPPRLAPTQVVIVPIWKSDEQKARIGEAARKAEAELAATVRVKADLREEISPGWKY
                     NEWELKGVPLRLEIGPRDLDASQFTAVRRMDRKKTPVPFADLPRTVTSMLEEIQKDMT
                     EVRRKDLVSRTYVATDYQAFQKDLDEKAGFYRAGWCGDGGCEAKIKEETKATIRCIPL
                     TGNKEEGQCLRCNVPSHEWVLFARAY"
     gene            3209..4309
                     /locus_tag="VFD06_03030"
     CDS             3209..4309
                     /locus_tag="VFD06_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN02541.1"
                     /translation="MLEGTIRITVEASTSQDAQIARVSIFADDRLLTILEKSPYTLTW
                     NSGAGQVLRKLRAVAEDSLGRSGEAVLIARRIGGVQYEDVRLVQVYAAVRDAKGRAVT
                     DLERSAFTLKEDGVPQTIATFAPARVPITIALLLDTSSSMRLGGRIDFARRGAETFLG
                     AVAPEDRMLVLAFDDTVKGDRDPTTDRKTLKQRIDAVTPGGGTALYDALYQTAEILTG
                     IEGRRAIVLLSDGRDQALVENEPGSLHVFEEALERVQRSDATVYAIGLGARLDQEMDL
                     ERRRSVKELLTILATQTGGRVYLPQRPGVLDDVYRQVAADLKDQYLLAYSSTNTARDG
                     RWRAIDVAVSDPALKVTARAGYFAPGAPSAVR"
     gene            complement(4273..4602)
                     /locus_tag="VFD06_03035"
     CDS             complement(4273..4602)
                     /locus_tag="VFD06_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015496274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HZN02542.1"
                     /translation="MRERLFDVDAWVIFGLLGQAFFTSRFVVQWVVSERKGRSTVPVA
                     FWYLSMAGGAILFAYAFLYRHDLVFTVGQAAGLFVYGRNLMLIRRAEVPGVVPIQRTA
                     EGAPGAK"
     gene            complement(4595..5323)
                     /locus_tag="VFD06_03040"
     CDS             complement(4595..5323)
                     /locus_tag="VFD06_03040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN02543.1"
                     /translation="MSAERDDLDLSLVIPVYNERDNLEPLVSEIERSLLGQGWRYEIL
                     MVDDGSVDGSGEALLALRRAHPVLRVIRFARNAGQTAAMDAGFHRSRGAIVVTLDADL
                     QNDPADIPNLVRAMDGWDAVVGVRTGRRDTLVRRVSSRVANYVRNRLSDETIEDTGCS
                     LKAYRREALDRLKLYNGLHRFLPTLLRMEGFRVRELPVGHRPRIHGRSKYGISNRLVP
                     SFLDLLAVRWMKKRSLRYEVRDDA"
     gene            complement(5320..5973)
                     /locus_tag="VFD06_03045"
     CDS             complement(5320..5973)
                     /locus_tag="VFD06_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02544.1"
                     /translation="MTTRPKAVPDDRAFLSAGLKVRVLKRGPWGKADILHLSHDGTVM
                     ILKDFSEKSTLVRWFGRRQLRRERRALRRLQGIDGIPAILGEIPPCGLLLQPMPGEAI
                     TRWRRRRPEEIDPMLARLQALVTRIHERGVAHLDLRKRDNILVDASGKPSVIDFNASI
                     AFHPGSIGARLVFPWLVRVDRAALLKWRSFLLPDHLTPAERRRHRRMSRLRRLWIFN"
     gene            6012..6677
                     /locus_tag="VFD06_03050"
     CDS             6012..6677
                     /locus_tag="VFD06_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02545.1"
                     /translation="MVPRHFNTAPPVPLSRGLLERLPRRSLHAGRNVSKASIDIVEHD
                     GRALVVKDVAHRPFWVRAVLGPFQLRREARAYRRLDGLEGIPSLVQVIDRRALALERI
                     DGRAIRDVPRGAVPTVFFDRLQALVAAIHARGVAHGDLHHGDVLVAPGDRPYVIDFST
                     ASFAGRHGSRTRRFFFDQARLADVRAVAKLRRRWLGAAEAAVPERPVLYRIGTTVRRL
                     LGL"
BASE COUNT         1145 a         2662 c         2593 g         1097 t
ORIGIN      
        1 cgctggccgc ggacgccccg cgccgccatc gcgctgcaga agtcgctccg cccgcgcctg
       61 agcctcctgg gaggcccgcg atcgccgcgc acggtggccg gcgccgacct ggcctatgac
      121 ctcgagcggg acaggctgca cgccgtcgtc gtcgtgttcc gcttcccatc gctcgaatgg
      181 gtcgagacga gcgccgtcac ccggcgcatc ccgtttccct acatcccggg cctcctgtcg
      241 ttccgggagg cgcccgcgat catcgcggcc tggcggcggc tgcgccgccc cgccgacctt
      301 ttgctgtgcg acggccaggg gatcgcgcat ccgcgcggcc tggggctcgc ctcgcacctc
      361 ggtctgctcc tcgacgttcc caccatcggg tgcgccaaga gcctgctcgt gggggagcac
      421 ggcccggtcg gttacgaccg ttccgcgacc acgccgctgc gccacgccgg gcgcatcgtc
      481 ggcgcggtcg tgcgcacgcg cgccggggtc gctccgatcg tcgtctcccc cggccaccgg
      541 atcggcgtcg cccgggcggt ccgcctggcg ctcgcgtgca cgcgcggctt ccgcatcccg
      601 gagccgacgc gacaggccga tctcctcgtc gggcgcctgc gccgcggcct tgaggctccg
      661 aggcctccga ggggacacgg atcgctgtgc tagaatccgt cgcgcatcga tgggccgcac
      721 cttcctcggc agtccacgcg ccgcaaggcg tccgggcgcg gtcggcgccg gagcgatcct
      781 catcgcattg atggtcaacg gggccgccac catggcagga ccggacagtg cctctcccga
      841 ccccctgcgc gaggcgcggc tgcgcatggt gagcgagcag atcgaggcgc gcgggatccg
      901 cgcgccggag gtcctggccg ccctgcgcgc cgttccgcga catctgttcg tccccgcgga
      961 ggtgcgcgag tcggcgtaca acgacggccc gctgccgatc ggccacggtc agacgatctc
     1021 gcagccgtac gtcgtcgcct tcatgaccga ggcggcgcgc atcaagcccg gcgatcgggt
     1081 cctcgagatc ggcaccggat cgggatacca ggcggcggtc ctcgacgcga tcggagcgcg
     1141 cgtctggagc atcgagatcg tcccggatct cgcggagcgg gcgcgccgcg ccctcgccgc
     1201 caccggggcg tcgcgcgtca cggtgcgcgc cggcgacggc accggaggct ggccggagga
     1261 ggcgccgttc gacgccatcg tggtgacggc ggcgccggag caggtgccgc aggcgctcct
     1321 ggaccagctg aaggtcggcg gacggctggt gatcccggtc ggctcggggt cgcaggaact
     1381 cctccgcatc acccgcaccg ggaccggctt cgagcgcgag ccgctcctgt cggtccgctt
     1441 cgtgccgatg accggcgagg cacgccggcc gcgcccctga tcggcacacc ggacggagac
     1501 gcgatgtcca acaggttcca cgggagaccc gcatgtcgaa ggtgatcacg ccgcgcgccg
     1561 aagactactc acgctggtac accgacgtcg tcctgcagtc gcagatggcc gactactcgc
     1621 cggtgaaggg gtgcatggtg atccgcccgc tcggctacgc cgtgtgggag gcgatgcagc
     1681 gtgagctcga cgcccgcttc cgcgccaccg gacaccagaa cgcctacttc ccgctgctca
     1741 tcccggaatc gttcatgaag cgggaggcgg agcacgtcga ggggttcgcg ccggagtgcg
     1801 ccgtggtcac gcacggcggc ggcaagaagc tggaggagcc gctgatcatc cgcccgacct
     1861 ccgaaaccat catctacgag atgtactcga agtggatccg ctcctaccgc gacctgccgc
     1921 tgctgatcaa ccagtgggcc aacatcgtgc gctgggagat gcgcacgcgc ctcttcctgc
     1981 gcaccaccga attcctctgg caggaggggc acacggcgca cgccaccgaa gccgaggccg
     2041 aggaggaggt gctcaagatc ctcgagatct accgctcgtt cgccgaggac tgtatggcgg
     2101 tgccggtgta cacggggcgc aagaccaaca gcgagaagtt cgccggggcg ctccggacct
     2161 actgcatcga ggcgctcatg caggacggca aggcgctgca ggccgggacg tcgcacagtc
     2221 tcgggcagaa cttcgcaaag gcgttcgacg tcaagttcca gaccacggcc ggggactggc
     2281 agcatgtctg gaacacctcg tggggggtgt ccacgcggct cgtcggggcg gtggtgatgt
     2341 cgcacggcga cgacaacggc gtcgccttcc cgccgcgcct ggcgccgacc caggtggtga
     2401 tcgtgccgat ctggaagtcg gacgagcaga aggctcgcat cggcgaggcg gcccgcaagg
     2461 cggaggcgga gctcgcggcg acggtgcggg tgaaggccga cctgcgcgag gagatttccc
     2521 cgggctggaa gtacaacgag tgggagctca agggggtgcc gctgcgactg gagatcgggc
     2581 cgcgcgatct cgacgcgagc cagttcacgg ccgtgcgccg catggaccgg aagaagaccc
     2641 cggtgccgtt cgccgatctc ccccggaccg tgacgtccat gctcgaggag atccagaaag
     2701 acatgaccga ggttcgccgg aaggatctgg tctcgcgcac ctacgtcgcg accgactacc
     2761 aggccttcca gaaggacctc gacgagaagg cgggcttcta ccgcgcggga tggtgcgggg
     2821 atggggggtg cgaggcgaag atcaaggaag agacgaaagc caccatccgc tgcattcctc
     2881 tgaccgggaa caaagaagag ggccagtgcc ttcgctgcaa cgtcccatcc cacgaatggg
     2941 tgttgttcgc acgcgcctat tagacgtgcg gaagtcgcgg cggggtcggg ggggcgctcc
     3001 ctccggcgcc ttacagccgg ccgggggcct cgcgcggacc gcgggcgcga tcctgatcgt
     3061 cttcgcaacc atcaccgcgg tggttcaggc gcagtcgccc ccggcggctg aaggctcccg
     3121 gagggagacg ccggacggcg cggcggcgac agcaagcaca ccctcagctt cgcctgtcaa
     3181 agttcggttt gtcgagccgg gcgctgcgtt gctcgaaggg acgattcgga tcaccgtcga
     3241 ggcctcgaca tcgcaggacg cgcagattgc gcgcgtctcc atcttcgccg acgacaggct
     3301 cctgaccatc ctcgagaagt cgccctacac gctcacctgg aattccgggg ccgggcaggt
     3361 gctccggaag ctgcgcgccg tcgcggagga ctcgctgggg aggtcggggg aggcggtgct
     3421 gatcgcgcgg cggatcggcg gggtgcagta cgaggacgtg cggctggtgc aggtgtacgc
     3481 ggcggtgcgg gacgcgaagg ggcgggcggt gaccgacctc gagcgctccg cgttcacgct
     3541 caaggaggac ggggtgccgc agacgatcgc caccttcgcg ccggcgcgtg tcccgatcac
     3601 catcgcgctg ctcctcgaca ccagcagcag catgcggctc ggcggccgga tcgacttcgc
     3661 gcgccgcggg gcggaaacgt tcctcggcgc cgtggcgccc gaggaccgga tgctcgtgct
     3721 ggcgttcgac gacacggtca agggggatcg cgatccgacg accgaccgga aaacgctcaa
     3781 gcagcggatc gacgccgtca ctcccggcgg cggcaccgcc ctctacgacg cgctctacca
     3841 gaccgcggag atcctcaccg ggatcgaggg gcggcgggcg atcgtcctgc tctccgacgg
     3901 ccgcgaccag gcgctcgtgg agaacgagcc ggggagcctg cacgtcttcg aggaggcgct
     3961 cgagcgggtg cagcgctccg acgcgacggt gtacgccatc ggcctcgggg cgcgtctcga
     4021 tcaggagatg gacctggaac ggcgccgcag cgtcaaggag ctgctcacga tcctggcgac
     4081 gcagaccggt gggcgcgtct acctgccgca gcgtcccggg gtcctcgatg acgtctaccg
     4141 gcaggtggcg gccgacctca aggaccagta cctgctcgcc tactcctcca ccaacacggc
     4201 gcgggacggg cgctggcgcg ccatcgacgt ggcggtgtcc gatccggccc tcaaggtgac
     4261 ggcgcgggcc ggctacttcg cgcccggcgc cccttccgcc gtccgctgaa tcggcacgac
     4321 cccggggacc tccgcccggc ggatcagcat gaggttcctt ccgtagacga acagtcccgc
     4381 cgcctggccg accgtgaaga cgaggtcgtg ccggtacagg aaggcatagg cgaagaggat
     4441 cgcccccccc gccatgctca ggtaccagaa ggccaccgga accgtgctcc gtcccttccg
     4501 ctccgagacg acccactgca ccacgaagcg ggaggtgaag aaggcctgtc ccagcaggcc
     4561 gaagatcacc caggcgtcga cgtcgaacag ccgctcacgc atcgtccctc acctcgtaac
     4621 gcaacgaacg tttcttcatc caccgcacgg ccagcagatc caggaaagac ggcaccagcc
     4681 ggttggagat cccgtacttc gaccggccgt ggatgcgcgg ccgatgcccg acgggcagct
     4741 ccctcactcg gaacccctcc atcctgagga gcgtcggcag aaagcggtgc aggccgttgt
     4801 agagcttcag gcgatcgagc gcctcgcgcc ggtacgcctt gagcgagcag ccggtgtcct
     4861 cgatcgtctc gtcgctcagg cggttgcgca cgtagttcgc gacccgcgac gagacccggc
     4921 gtaccagcgt gtcgcgccgg ccggtgcgca cgccgacgac cgcgtcccag ccgtccatgg
     4981 cgcgcaccag gttcgggatg tcggccgggt cgttctgcag atcggcgtcg agggtcacga
     5041 cgatcgcccc gcgggagcgg tggaagccgg catccatcgc cgccgtctgg ccggcgttgc
     5101 gcgcgaagcg gatcacccgc aggaccgggt gggcgcgccg cagcgccaga agcgcctcgc
     5161 cgctgccgtc gacgctgcca tcgtccacca tcaggatctc gtagcgccac ccctggccca
     5221 gcaggctccg ctcgatctcg gagacgagcg gctcgagatt gtcccgctcg ttgtagaccg
     5281 ggatcacgag cgacaggtcg aggtcgtccc gctcggcgct caattgaaga tccacaggcg
     5341 ccgcaggcgg ctcatgcgac ggtgccggcg ccgctcggcg ggggtgaggt ggtccggcag
     5401 gaggaacgac cgccacttca ggagcgccgc ccggtcgacc cgcacgagcc acgggaagac
     5461 gagtcgcgcg ccgatcgatc ccgggtggaa cgcgatgctg gcgttgaagt cgatgacgct
     5521 cggcttgccg gaggcatcga ccaggatgtt gtcccgcttg cgcaggtcga ggtgcgcgac
     5581 gccccgctcg tggatgcgcg tcaccagcgc ctgcagccgt gccagcatcg ggtcgatctc
     5641 ctcggggcgc cgccggcgcc accgggtgat cgcctccccc ggcatcggct gcagcagcag
     5701 cccgcacgga gggatctccc ccaggatcgc cgggatgccg tcgatcccct gcagccggcg
     5761 cagcgcccgg cgctcgcggc gcagctggcg gcgcccgaac cagcgcacga gcgtgctctt
     5821 ctccgagaag tccttcagaa tcatcaccgt gccgtcgtgg ctcaggtgca ggatgtcggc
     5881 cttcccccac ggcccgcgct tgaggacgcg caccttgagg ccggcggaga ggaaggcccg
     5941 gtcgtcgggg accgccttcg gacgggtcgt cacggacagc gtgatctcct gtgctattct
     6001 cggccggctc gatggttccg cgtcatttta atacagcgcc cccggtcccc ctctcccggg
     6061 gcctgctgga gcgacttccg cgccggagcc tccacgccgg gcgcaacgtc tccaaggcct
     6121 cgatcgacat cgtcgagcac gacgggcgcg ccctggtcgt caaggacgtg gcccaccgcc
     6181 cgttctgggt gcgggcggtc ctcggaccgt tccagctgcg ccgcgaggcg cgcgcctacc
     6241 ggcgcctcga cggcctcgaa ggcattccgt cgctcgtgca ggtgatcgat cgccgtgccc
     6301 tggcgctcga gcggatcgac ggacgggcga tccgcgacgt gccgcgcggc gcggtcccca
     6361 cggtcttctt cgaccggctg caggcgctcg tcgccgccat ccacgcgcgc ggcgtcgccc
     6421 acggggatct gcaccacggc gacgtcctgg tcgccccggg cgaccgtccc tacgtcatcg
     6481 acttctcgac cgcgtccttc gccggccgcc acggcagccg cacccgtcgc ttcttcttcg
     6541 accaggcgcg gctcgccgac gtgcgcgccg tcgcgaagct ccgccgccgg tggctcggcg
     6601 ccgccgaggc cgccgtgcct gagcggccgg tgctgtaccg gatcgggacc acggttcgaa
     6661 gattgctggg gttgtagtag cgccgagccg cccccctcca ggggtgacga gttccggaca
     6721 gggcatttgg ctccccaagc aaccgaattc gccgatttcg cgcaacctcg ggaggcgcat
     6781 cacgactaac aatagaagaa cgcggtccct caccttagaa ggaggtcgca tgaagagaat
     6841 gtcacgtgtg gtactggctt cgacccttgc agtgttgacc tttggagtcg cggaaccgac
     6901 cgatcccgtc gcgaccgaac tcagcgagga actcgctccc agcaccgggc tgtcatcggc
     6961 cgacgtccac gccgtcattc gcgacctgac gacgccggcc actctcgatt gtgccgcccc
     7021 aagcgtcgcg ctccccgagc tcaacggctc gatgggatcg ctcggtagca cgaacgatgc
     7081 gtcgtcctca ctccccagtc cgcccatgga cgacgaagga cccccgtgtg gcaggaccca
     7141 ctgctttccc tgcctcaacg gggcagccta ctgttgcaac gattgcggtt gcttctatgc
     7201 gtggtgcacc tgatcgacaa cggtcccacc tgcgcattct gcgccggccg caggtcgatc
     7261 tcggcgcggg cgctgggggc ctccaccaga ctcgcccctc tccgcaccgc ttgagcagcg
     7321 tgagatggaa catcgggaag ctggcggcag gccgcgacct acaatgacga ctcggagtgg
     7381 cgcagcgagg gcgctctcgc tgtggtcgaa ctaccggccc ccgtactgac ggcggtagta
     7441 gtcgcggtac tcgcccgact tgatcggctc ccaccaggag cggtggctcc ggtacca
//
LOCUS       DASRIQ010000128         3318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2128015, whole genome shotgun sequence.
ACCESSION   DASRIQ010000128 DASRIQ010000000
VERSION     DASRIQ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128015"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFD06_03055"
     CDS             <1..297
                     /locus_tag="VFD06_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN02546.1"
                     /translation="GIGAPDLAALLYLGAIQIGLAYALLTTGLARVRAVDASLLLFLE
                     PVLNPVWAFLVHGERPPGAAIAGGLLILGATFVKTVYDLRRAPPPSAAGVAPRD"
     gene            332..865
                     /locus_tag="VFD06_03060"
     CDS             332..865
                     /locus_tag="VFD06_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN02547.1"
                     /translation="MHTFVKQLRRSMPALAFPMLVLGCGGASGAEEVRTLVQREAEAI
                     QAHDLKGLERIWSERPDIVLIDVTPPGRFEGWTAIARALGDFMTRTSDVRMTVERLNV
                     EARGDLAVATYDWTMTGKVGEQSLEDRGAATSIYRKEEAGWRLVHAHYSASPRGAAPA
                     PAGAGAVPPGGAAKPQG"
     gene            869..>3318
                     /locus_tag="VFD06_03065"
     CDS             869..>3318
                     /locus_tag="VFD06_03065"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZN02548.1"
                     /translation="MAEERVTREVLPNGLTVLVAEDAASPVVAINLWVQAGYFDETDE
                     EIGISHVIEHMFFKGTTARPRPDQIATEMKALGGETNAGTYYESTNYHVILPAERFEA
                     ALSIQADALTDPLFDADELKREKEAVLQEARRKLDTPQAYSLEMLYREAFDVHRIRRW
                     RIGTEEGIRALTRDQMVHYYRRHYVPGRIVLSIAGGVVTPRALEAARALFGAIPAGEG
                     ADLGSPPEPARTGFRTARLSGDIRRAQMVLGFRSAPILSDEDPALRVLAHVLGGGRAS
                     RLHQAVKERDGLVDGIGAGVEAFRDLGIFTVHIEGDPKHAAAALRSTIAEIERLRTEP
                     PSAAEIERARTAIEFRYHQSRSEALGRSAILAYYEALGGYGLAEESMRRMLAVTPAEV
                     RRAAEATLVVRNATLLEYVPESSAAPAPPEAEARLQELLRVEPGTPLKDPLATRVGPA
                     PPAVPPLLRLHPLPPAGGVTRRRFENGPLLVHEERRDQPLVTLCVAFRGGRAGEQREQ
                     SGVTRLMQASMLKGSPGRDARRVAVELDSLGTAVERVVDEDYFGFAMAVLSRHAAKGF
                     EILMDLVRRPAFAYEEIEKERALLLAAQESIRDQSLPHTFQLFRQAAFGSHPYSQPAY
                     GLTASVQSLKREDLVRWHRLTIRPASMLVSVVGALGPNEAADLVGQAIAEWPQDGFGG
                     SEPGALLPWGAAEVVETRRRAQTAQIIGFPTPGVQSPERHALDIVQSVTSGLGGRFFE
                     AVRGKRGLAYAVQSFNFHRLRGGAFLTYLGTSPRDEAEARRVLFQEIATLRQDGPRRD
                     EIERARRHLIGGHAIGMQG"
BASE COUNT          454 a         1217 c         1202 g          445 t
ORIGIN      
        1 gggatcggcg caccggatct cgccgcgctc ctgtatctcg gggcgattca gatcggcctg
       61 gcgtacgcgc tcctgaccac ggggctggcg cgcgtgcgcg ccgtcgacgc gtcgctgctg
      121 ctgttcctcg agccggtgct gaatccggtg tgggccttcc tcgtgcacgg cgagcggccg
      181 cccggcgcgg cgatcgccgg gggactcctc atcctcggcg ccacgttcgt caagacggtg
      241 tacgacctgc gccgcgcgcc gccgccaagc gcggcgggcg tcgcgccgcg cgattgacgg
      301 acccggatcc gtacgttaga gttcagccgg catgcacacg ttcgtgaagc agctccggag
      361 aagcatgccg gccctcgcgt ttccgatgct cgtcctcggc tgtggcggag cgtccggcgc
      421 cgaagaggtg cgtacgctcg tccagcgcga agcggaggcg atccaggcgc acgacctcaa
      481 ggggctcgag cgcatctggt cggagcgccc cgacatcgtc ctcatcgacg tcaccccgcc
      541 cggccgcttc gagggctgga ccgccatcgc ccgcgccctc ggcgacttca tgacgcgcac
      601 ctccgacgtg cgcatgacgg tcgagcgcct caacgtcgag gcgcgcggcg atctcgccgt
      661 cgcgacctac gactggacca tgaccggcaa ggtgggggag cagagcctcg aggatcgcgg
      721 cgcggcgacc tcgatctacc gcaaggagga ggccggctgg aggctggtgc acgcccatta
      781 ctccgcctcc ccccgcggcg cagcgccggc ccccgccggc gcgggcgcgg tgccgcccgg
      841 cggcgccgcg aagccccagg gctagaccat ggcggaagaa cgggtcaccc gggaagtcct
      901 gccgaacggc ctgacggtgc tggtcgccga ggacgccgcc tcgccggtcg tcgccatcaa
      961 cctctgggtg caggccggct acttcgacga gaccgacgag gagatcggca tctcccacgt
     1021 catcgaacac atgttcttca aggggacgac cgcccgcccg cgccccgacc agatcgccac
     1081 ggagatgaag gcgctcggcg gcgagaccaa cgccggcacc tactacgaat ccaccaacta
     1141 ccacgtcatc ctccccgccg agcgcttcga ggcggcgctg tccatccagg ccgacgcgct
     1201 caccgacccg ctgttcgacg ccgacgagct gaagcgcgag aaggaggcgg tccttcaaga
     1261 agcccgccgc aagctcgaca cgccgcaggc gtactcgctc gagatgctgt accgcgaggc
     1321 gttcgacgtg caccgcatcc ggcgctggcg catcggcacc gaggagggga tccgggcgct
     1381 gacccgcgac cagatggtgc actactaccg ccggcactac gttcccgggc ggatcgtcct
     1441 gtcgatcgcc ggcggggtcg tgacgccgcg cgccctcgag gcggcgcgcg ccctgttcgg
     1501 ggcgatccct gcgggggaag gggcggacct gggatcgccg ccggagccgg cgcgcaccgg
     1561 gttccgcacc gcgcgtctct ccggcgacat ccggcgcgcg cagatggtcc tgggcttccg
     1621 ctccgcgccc attctgagcg acgaggaccc ggcgctgcgg gtgctggcgc acgtgctcgg
     1681 gggcggacgg gcctcgcgcc tgcaccaggc ggtcaaggag cgggacggcc tcgtggatgg
     1741 catcggcgcc ggcgtggagg cgttccgaga cctcgggatc ttcacggtgc acatcgaagg
     1801 ggacccgaaa cacgcggcgg cggcgctgcg ctcgacgatc gcggagatcg agcggctgcg
     1861 gaccgagccg ccgagcgccg ccgagatcga gcgggcgcgc acggcgatcg agttccgcta
     1921 ccaccagagc cgctccgagg cgctcgggcg ctccgccatc ctcgcctact acgaggcgct
     1981 cggcgggtac ggcttggcgg aggagtcgat gcgccgcatg ctcgccgtca cccccgcgga
     2041 ggtgcgccgc gccgccgagg cgacgctcgt cgtccggaac gcgacgctcc tcgagtacgt
     2101 gccggaatcg tcggccgctc cggctccccc cgaggccgag gcacggctcc aggaactgct
     2161 ccgcgtcgag ccgggcacgc cgctcaagga tccgctcgcg acgcgcgtcg gcccggcgcc
     2221 ccccgccgtg ccgccgctgc tgcgcctcca cccgctccca ccggccggcg gcgtgacgcg
     2281 tcggcggttc gagaacggcc cgctgctcgt ccacgaggaa cgccgcgatc agccgctcgt
     2341 cacgctgtgc gtcgcgtttc ggggcggtcg cgccggcgag cagcgcgagc agagcggtgt
     2401 gacccgcctg atgcaggcgt cgatgctcaa ggggagcccg gggcgcgacg cgcgccgcgt
     2461 cgcggtcgag ctcgacagcc tcgggaccgc ggtggagcgg gtggtggacg aggactactt
     2521 tggcttcgcc atggcggtgc tgtcgcgcca cgcggcgaag gggttcgaga tcctcatgga
     2581 tctcgtgcgc cgcccggcgt tcgcgtacga ggagatcgaa aaggagcgcg cgctgctgct
     2641 ggcggcgcag gagtcgatcc gcgaccagtc gttgccgcac acgttccagc tcttccggca
     2701 ggccgccttc ggcagccatc cgtactccca gccggcgtac ggcctgaccg cgtcggtcca
     2761 gtcgctcaag cgcgaagacc tggtgcgctg gcaccggctg acgatccgcc ccgcctcgat
     2821 gctcgtctcg gtggtcgggg cgctgggtcc gaacgaagcg gccgacctcg tcggccaggc
     2881 gatcgccgaa tggccgcagg acggcttcgg cgggtcggag ccgggggcgc tcctcccctg
     2941 gggagcggcg gaggtggtcg agacccgccg gcgcgcgcag acggcgcaga tcatcgggtt
     3001 cccgacgccg ggcgtgcagt cgccggagcg gcacgcgctc gacatcgtcc agtcggtcac
     3061 gtccggcctg ggagggcggt tcttcgaggc ggtgcgcggc aagcggggcc tcgcctacgc
     3121 cgtgcagtcg ttcaatttcc accggctgcg cggcggcgcc tttctcacct acctcggcac
     3181 ctcgccgcgc gacgaggccg aggcgcgccg cgtcctcttc caggagatcg ccacgctgcg
     3241 ccaggacggc ccgcgccgcg acgagatcga gcgggcgcgg cggcacctca tcggcggaca
     3301 cgccatcggg atgcaggg
//
LOCUS       DASRIQ010000129         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_313564, whole genome shotgun sequence.
ACCESSION   DASRIQ010000129 DASRIQ010000000
VERSION     DASRIQ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313564"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1182
                     /gene="alr"
                     /locus_tag="VFD06_03070"
     CDS             64..1182
                     /gene="alr"
                     /locus_tag="VFD06_03070"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZN02549.1"
                     /translation="MTSGRATWIEIDLGALAHNLKVVRAHLSGARVMAVVKADAYGHG
                     AIEVARRLEREGADWLGVALVEEGIELRRAGVELPILVLGGFAPPQAQAILDHRLTPS
                     LFRLDQVEALHDAAARRGVVADAHVKIDTGMGRLGLPAREAGAFAARLAAANGIRVTG
                     AFSHFPAADTSGDVSTTRQTQSFLQAVAALKAAGLSPSDLHLANSPAVLDHRATWMTL
                     ARPGLLLYGYHPDGCARTLAVRPVLALRSAVVDLRDLQSGDTVGYGRTFKAPGPVRIA
                     TLGLGYADGLPRAASNRAEVLLAGRRAPIVGRISMDLTTVDVTAIPQTAVGDVATVIG
                     EQEGDRIGADALARACGTISWEILARLGPRLPRRHVGI"
     gene            1193..2227
                     /locus_tag="VFD06_03075"
     CDS             1193..2227
                     /locus_tag="VFD06_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZN02550.1"
                     /translation="MNNLSARLALLALSALFASMARPPSLSPLDLTGPALGLVAGGII
                     LGLERLLRDLRLRVLLGGVAGLIVGGLVATLLLALLPAESLRGVPPAATRGLVLLLFG
                     YLGLTAGASRAGRFSPAGLRAAWSEGTDGRNTKILDTSAIIDGRIADVAETGFLEGSL
                     VVPQFVLRELQQIADSADPLKRNRGRKGLEILQRLKKGSDSRVVLSEADFAGIADVDM
                     KLIELARQTEGRILTNDFNLNKIAELRGVRVLNVNTLANALKPVVLPGESMNLFILKE
                     GKEPNQGVGYLDDGTMVVVDNAKRFLGRNIAITITSVLQTTAGKMFFGRCEAAEEIAF
                     PENRGRRTPA"
     gene            2224..3243
                     /locus_tag="VFD06_03080"
     CDS             2224..3243
                     /locus_tag="VFD06_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02551.1"
                     /translation="MSSAPRLVSAAAALLLLGLAGVTQARAASPIGFVILPFENTAEV
                     EALDWLQNGLAFHTGEHLKACGASVVEEEDRAVFLEGNGIPQGASLTLASALELGRKM
                     RARSTGLRPDRLVLGRYSVQDGEFTVQVRVIDLEEERARPWLMRQGRLLNLLSVHAGL
                     MEGLGRDAGMRGCGGEESTPAPPLLAFETYTRAMAESEPKKRLGLLRRALQEFPGYPQ
                     AAYQAALLQARAERWDEASTTLQSIAGEPIPYATDAHLLSAAVALQRKDAPTAAAQAR
                     LALQRVPSARGHALLGRALAAQGDLPGARKEQERAQAIDPSEPEIEDLRRILDGGTPL
                     GGKPQ"
     gene            3240..>3659
                     /locus_tag="VFD06_03085"
     CDS             3240..>3659
                     /locus_tag="VFD06_03085"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZN02552.1"
                     /translation="MTLTDLDIARRARLVRIEALAESLGLQPEEILPYGRYKAKIALK
                     ALERLRDRPDGRLICVTGINPTPLGEGKTVVTIGLTQALRRLGKRALSTLRQPSMGPV
                     FGVKGGATGGGYSQVVPNEEINLHFTGDFHAVATAHNL"
BASE COUNT          498 a         1315 c         1337 g          509 t
ORIGIN      
        1 caccatcgag ttcgccttcc tgcgcgagta cacccggttc gagcagcggg agtggcgtcc
       61 ggagtgacgt ccgggcgggc cacctggatc gagatcgacc tcggcgccct ggcgcacaac
      121 ctcaaggtcg tccgcgccca cctgtcgggg gcccgcgtca tggcggtcgt caaggcggac
      181 gcctacgggc acggcgccat cgaggtggcg cggcgcctgg agcgggaggg ggccgactgg
      241 ctcggcgtcg cgctggtcga ggaggggatc gagctgcggc gggccggagt ggagctgccg
      301 atcctcgtgc tcggcgggtt cgcgcccccc caggcgcagg cgatcctcga tcaccggctc
      361 accccgtctc ttttcaggct cgaccaggtg gaggcgctcc acgacgcggc ggcgcggcgc
      421 ggcgtggtcg ccgacgcgca cgtgaagatc gacaccggca tggggcgcct gggcctcccc
      481 gcccgggagg ccggagcctt cgccgcccgg ctcgccgcgg cgaacgggat ccgggtcacc
      541 ggggccttct cgcacttccc cgccgccgac acctccggcg acgtctcgac gacgcggcag
      601 acgcagtcgt tcctgcaggc ggtggcggcg ctgaaggcgg ccgggctctc cccgtccgat
      661 ctgcacctcg ccaacagccc ggcggtcctc gatcaccgcg ccacctggat gacgctggcc
      721 cgcccggggc tcctgctcta cgggtaccac ccggacggat gcgcgcggac gctggcggtg
      781 cgtccggtgc tggcgcttcg aagcgccgtc gtcgatctgc gcgacctgca gtcgggcgac
      841 acggtcggat acggccgcac cttcaaggcg ccgggaccgg tgcgcatcgc caccctcggc
      901 ctcggctacg ccgacggcct gccgcgcgcc gcgtcgaacc gggcggaggt gctgctcgcg
      961 gggcggcgcg ccccgatcgt cggccgtatc agcatggatc tgaccacggt cgacgtgacc
     1021 gcgatcccgc agacggcggt cggcgacgtc gcgactgtga tcggcgagca ggagggggat
     1081 cggatcggcg ccgacgccct ggcgcgcgcc tgcggcacca tttcgtggga gattctggcg
     1141 cgcctcggtc cgcggctgcc gcggcggcat gtcgggatat gatgggccgg ccatgaacaa
     1201 cctgagtgcc cggctcgcgc tcctggctct gtccgccctg ttcgcctcga tggcgcgccc
     1261 tccatccctg agtccgctcg atctgaccgg gccggcgctg ggactggtcg cgggcgggat
     1321 catcctcggg ctggagcggc tcctgcgcga cctgcgcctg cgggtccttc tgggaggcgt
     1381 ggccggtctc atcgtcgggg ggctggtggc gacgctcctc ctcgcgctcc tgccggccga
     1441 gtcgctgcgc ggcgtgccgc cggcggcgac gcgcggtctc gtgctgctgc tgttcggcta
     1501 tctcggcctc accgccgggg cctcgcgcgc cgggcgtttc tcccccgccg ggctgcgcgc
     1561 cgcctggagc gaggggacgg acggacgcaa caccaagatc ctcgacacca gcgcgatcat
     1621 cgacggacgg atcgccgacg tggcggagac cggcttcctc gagggatcgc tggtcgtgcc
     1681 gcagttcgtc ctgcgggaac tgcagcagat cgcggactcc gccgatccgc tgaagcgcaa
     1741 ccgcgggcgc aaggggctcg agatcctcca gcggctcaag aaggggtcgg actcgcgcgt
     1801 cgtcctgtcc gaggccgact tcgccgggat cgccgacgtc gacatgaagc tcatcgagct
     1861 ggcgcgccag acggagggcc gcatcctgac caacgacttc aacctcaaca agatcgccga
     1921 gctgcgcggg gtgcgcgtgc tcaacgtcaa cacgctggcg aacgccctca agccggtcgt
     1981 ccttcccggg gagtcgatga acctgttcat cctcaaggag gggaaggagc cgaaccaggg
     2041 ggtcggctac ctcgacgacg gcaccatggt cgtggtcgac aacgccaagc ggttcctcgg
     2101 gaggaacatc gccatcacca tcacctcggt gctgcagacg acggccggga agatgttctt
     2161 cgggcgctgt gaggcggccg aggagatcgc cttccccgag aaccgcggcc ggcggacccc
     2221 ggcgtgagtt cggcaccgcg cctcgtttcc gccgcggcgg cgctcctcct cctcggcctc
     2281 gccggggtga cccaggcgcg cgccgcctcc cccatcggct tcgtcatcct gccgttcgag
     2341 aacaccgcgg aggtcgaggc gctcgactgg ctgcagaacg gcctggcgtt ccacaccggc
     2401 gagcacctca aggcctgcgg cgcctcggtg gtcgaagagg aagatcgggc ggtgttcctg
     2461 gaggggaacg gcattcccca gggcgcgtcg ctgacgctcg cgagcgccct cgagctcgga
     2521 cggaagatgc gggcgcggtc cacggggctg cgcccggacc ggctggtgct gggacgctac
     2581 tccgtgcagg acggggagtt cacggtccag gtccgggtga tcgatctcga ggaggagcgg
     2641 gcgcggccgt ggctcatgcg ccaggggcgc ctcttgaatc tgctgtcggt ccacgctggg
     2701 ctcatggaag ggctcgggcg cgacgccggc atgcgcggct gcggcggcga ggagagcacc
     2761 ccggccccgc cgctcctcgc cttcgagacc tacacgcgcg ccatggcgga gagcgaaccg
     2821 aagaagcggc tcggcctcct gcgccgggcc ctgcaggagt tccccggcta tccgcaggcc
     2881 gcgtatcagg ccgcgttgct gcaggcgcgc gcggagcgct gggacgaggc gtcgaccacg
     2941 ctgcagtcga tcgccggaga gccgatcccg tacgccaccg atgcgcatct cctgagcgcc
     3001 gccgtcgcgc tgcagcgcaa ggacgcgccc acggccgcgg cccaggcgcg gctggcgctg
     3061 cagcgcgtcc cgtcggcgcg cgggcacgcc ctgctcggcc gggcgctggc ggcgcagggg
     3121 gacctgccgg gggcgcgcaa ggagcaggag cgggcccagg ccatcgatcc gtccgagccc
     3181 gagatcgagg atctccggag gatcctcgac ggcgggacac cattgggagg gaagccacag
     3241 tgacgttgac cgacctcgac atcgcgcgcc gcgcgcggct cgtccgcatc gaggcgctcg
     3301 ccgaaagcct cggcctgcag ccggaagaga tcctcccgta cgggcgctac aaggcgaaga
     3361 tcgcgctcaa ggccctcgaa cgcctgcgcg accgtccgga cggccggctc atctgcgtca
     3421 ccggcatcaa cccgacgccg ctcggcgagg ggaagacggt cgtcaccatc gggctcaccc
     3481 aggcgctgcg gcgtctcggc aagcgggccc tcagcacgct ccgtcagccg tcgatggggc
     3541 cggtgttcgg cgtgaagggc ggggcgaccg gcggcggcta ctcgcaggtg gtgccgaacg
     3601 aggagatcaa tctccacttc accggcgact tccacgccgt cgccacggcg cacaacctg
//
LOCUS       DASRIQ010000130         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2392201, whole genome shotgun sequence.
ACCESSION   DASRIQ010000130 DASRIQ010000000
VERSION     DASRIQ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392201"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFD06_03090"
     CDS             complement(<1..634)
                     /locus_tag="VFD06_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02553.1"
                     /translation="MSAIQGRRVPRRARALSAALFVAATALPGCALTRRYDMARIHAD
                     AAAREDRNPDRNPVIFIHGFIGAKLRNERTSEGVWGRLANAITRSQVDDLDLPIEAAD
                     LKNNRDHLVPYALYESVAGVKFYGAMIDALETTGGYRIGDIRDPQPGDTGFVYVYDWR
                     RDNVESATGLAEAIHRVRTRLKAPQMRFDIVAHSMGGLVALYYLMYGGVDV"
     gene            complement(755..>2327)
                     /locus_tag="VFD06_03095"
     CDS             complement(755..>2327)
                     /locus_tag="VFD06_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M49"
                     /protein_id="HZN02554.1"
                     /translation="REPLEVKLERLRPAIFDPAYEPLLTCKTAPEGIDLLACSSVNYY
                     DGVTLADLRGFEEKNPLNSRLVKRDGRVVEEVWRAGRAAGTEGEAIPSGRYASQLRTV
                     IGFLTKAQVFAPPDEGSALRRLGDYFASGTPDEFRRYNLEWVTLNPSVDTVQGFIETY
                     HDPRGRKGGWQGMVFFSDPDRTRLLQGLAAEAHGFEERAPWDPRFKRRNFTPPVVAAV
                     EIVLAVGDGGPSPPIGVNLPNDDAIQEKVGNRSFLYANVLEASNRATLQALTEEFALP
                     EDRPMLQQHAVEAEILLTAMHEALGHAAGRSAEGLPAEPAVMLGETYAALEEARAELV
                     ALHAIFDPVLVERKLLSSPAVAEAAYRDYLLHDLSMLRRVRTGELLEDDHMRATHLIV
                     EWLRAHDGGVEVVSRDGRTYFRVADVPAMRKGVATLLAEVQRIKGEGDRAAARALLDR
                     YATRIDPRLRDEVVARADRAGIPSYMAFVMPEIVPVRDASGEVVDASLEPGGDFTLQM
                     LRFSGKLQREGDGPR"
BASE COUNT          337 a          815 c          829 g          346 t
ORIGIN      
        1 ccacgtccac cccgccgtac atgaggtagt agagggcgac gagccccccc atggagtgcg
       61 ccacgatgtc gaaccgcatc tgcggcgcct tcaggcgggt tcgcacgcgg tggatggcct
      121 cggcgagccc ggtggccgac tcgacgttgt cgcgccgcca gtcgtagacg tacacgaacc
      181 cggtgtcccc gggttgcggg tcgcggatgt cgccgatccg gtagccgccg gtggtctcca
      241 gcgcgtcgat catggcgccg tagaacttca cgccggcgac gctctcgtac agtgcgtacg
      301 ggaccagatg gtcgcggttg ttcttcaggt cggccgcctc gatcggcaga tcgaggtcgt
      361 ccacctgcga gcgggtgatg gcgttggcga ggcggcccca gaccccttcg gaggtacgct
      421 cgttgcgcag cttcgcgccg atgaacccgt ggatgaagat gaccggattg cgatcggggt
      481 tgcgatcctc gcgggcggcg gcgtccgcgt ggatccgggc catgtcgtag cggcgagtca
      541 gggcgcatcc cggaagggcg gtcgcggcga cgaacagcgc ggcggagagc gcccgggcgc
      601 gacgggggac acgacgaccc tgaatggcac tcactggctc agccccccgc tcccgccgga
      661 gccctgtcaa tatatggtcg gccccggggt caggcaatgc gacggaggcg gttggcgcgg
      721 agcacgccgc gcagggtgag ccgggacagg cacctcagcg cgggccgtcc ccttcacgct
      781 gcagtttacc cgaaaagcgc agcatctgga gggtaaagtc tcctcccggt tcgagcgagg
      841 cgtcgaccac ctcgccggag gcgtcccgca ccgggacgat ctccggcatg acgaaggcca
      901 tgtacgacgg gatgccggcc cggtcggcgc gggcgaccac ctcgtcccgc aggcgcggat
      961 cgatccgggt ggcgtaacgg tccaggagcg cgcgcgccgc ggcgcgatcc ccttcgcctt
     1021 tgatgcgctg cacctcggcc aggagcgtcg ccaccccctt ccgcatggcg gggacgtcgg
     1081 ccacccggaa gtacgtccgc ccgtcgcggg agaccacctc aacgccgccg tcgtgcgccc
     1141 gcagccactc gacgatgagg tgcgtggcgc gcatgtggtc gtcctcgagg agctccccgg
     1201 tgcgcacgcg ccggagcatg ctcaggtcgt ggagcaggta gtcccggtac gccgcctccg
     1261 ccacggcggg ggaggagagg agcttccgct ccacgagcac cgggtcgaag atggcgtgca
     1321 gtgccacgag ctcggctcgg gcctcctcga gcgccgcgta ggtctcgccg agcatcaccg
     1381 ccggctccgc cggcaggcct tccgccgacc ggccggcggc gtgcccgagc gcctcgtgca
     1441 tggcggtcag gaggatctcc gcctcgaccg cgtgctgctg gagcatcggg cggtcctccg
     1501 gcagggcgaa ttcctcggtc agcgcctgca gcgtggcgcg gttggaggcc tccagaacgt
     1561 tggcgtacag gaacgagcgg ttgccgacct tctcctggat cgcgtcatca ttcggaagat
     1621 tcacgccgat cggcggggac ggcccgccgt cgccgacggc gagcacgatc tcgaccgccg
     1681 ccaccaccgg cggcgtgaag ttgcgccgct tgaagcgcgg gtcccagggg gcccgttcct
     1741 cgaagccgtg cgcctccgcc gccagtccct gcaggagccg cgtgcgatcc gggtcggaga
     1801 agaaaaccat gccctgccag ccccccttgc ggccgcgcgg gtcgtggtag gtctcgatga
     1861 acccctgcac agtgtcgaca ctggggttga gcgtcaccca ctccaggttg tagcggcgga
     1921 actcgtccgg agtgccgctc gcgaagtagt cgccgagccg tcgtaaggcc gacccttcgt
     1981 cgggcggagc gaacacctgg gccttggtga ggaagccgat caccgtccgg agctgcgaag
     2041 cgtaccgccc ggaggggatc gcctccccct ccgttccggc cgcccgcccg gcgcgccaga
     2101 cctcctcgac gacccggccg tcgcgcttca ccagccggct gttgaggggg ttcttctcct
     2161 cgaagccgcg caggtcggcc agggtgacgc cgtcgtagta attgacgctg ctgcaggcga
     2221 gaaggtcgat cccctccggc gccgtcttgc aggtcaagag cggctcgtag gccgggtcga
     2281 agatcgccgg acgcagccgc tcgagcttca cctcgagcgg ctcgcgc
//
LOCUS       DASRIQ010000131         6877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2927511, whole genome shotgun sequence.
ACCESSION   DASRIQ010000131 DASRIQ010000000
VERSION     DASRIQ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2927511"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3348)
                     /locus_tag="VFD06_03100"
     CDS             complement(<1..3348)
                     /locus_tag="VFD06_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HZN02555.1"
                     /translation="MVEASAVASPAAVPVPPPVGWHGFRLAKLELFNWGTFDSSRGHI
                     HTVNPDGATTLLIGQNGSGKSTLVDAILTLLVRPVVRNYNVAAGAQKQERDERSYIRG
                     ACGRLGRDDDNRAEIQYLRGDRLHYSALLACFRNEAGAAFTVAQVLYLDGEGQPQKVY
                     CFSDEERSIAEHCANLPDTGKLRQQMERRGFRATTSYTEYYKWFARATGLRAKAMDMF
                     NQTVAVKDIQSLNRFIREHMLEAKPWGERIDALQSHFTQLSEAHRSLVRVRQQFELLL
                     PVEAKGKTYRAKAEEYAAAERMLAAADTFFRTKTVTLFVPECARLRGELEQNAARRCA
                     IDSELHDVAESIRTTRNEIEQSGGQRLREIPHLLRRARDGADAARAARRRFDEALRAA
                     GITDAVPDAAALAALRQRLPEAAARIAGDLRALDEERNRAVVDRDAVRRQEDEDQREL
                     AALAERQGNLPVALAEARRRLCEGVGVPERELPFVAELVAVRPDQRDWQPSIEMVLRG
                     FALSLLVPTRYYATVSGYVDRTRIEDRHGVGQRLTYLRVGETSSPPRNLPPLHPQSLV
                     RKLDFRDGQPLVARVRAELEARHNYQCCETVEEFQSARVSAVTRQRHVKRNEVRHDKD
                     DRDHALDPRRFVLGWDNREKRRLLAEGLVRLRGQARTLDERVQGLAAAMETLRHRNAA
                     LAQLGAVADFAAIDARPYERQAEALDREKAALEAGNQALRLLKQRLAEDEAAVARLQP
                     ERDACVVRQDRLSRAVLEGERALSNARHVLDERRASGDLDAHAESFAGIAALLADRPL
                     SAETLVEEEQGFGRQQRGLIDRLRSELEPERQALCDGMHHYLNKFPEDCADLAARVEY
                     LDSFCALCEQVRRDDLPQYEARFKERLNEKVTQEIGLLHGALQTERGEIESKIELLNR
                     SLRQLEYRPGTYIRLEPRPVRDREIADFQAGLRECLSGSFEGTPEADEARFVKIDKFL
                     ARLRDEQRWREKVTDVRRWFDFAAREMEEGTDVERVYYEDSAGLSGGEKAKLAFTILV
                     AAIAYQYDIDPGRPSSERFGFVVVDEMFSKVDDLYAQYALELFAKFGLQLLIVAPLDA
                     KARVTEPYVGCYLHVVKDPQANTSEIFS"
     gene            complement(3360..3983)
                     /locus_tag="VFD06_03105"
     CDS             complement(3360..3983)
                     /locus_tag="VFD06_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HZN02556.1"
                     /translation="MASNSPAQHATSELREWSVAAVKLLQGVVYDDEPKVWDVLLRSK
                     SALDSYFGRIGLSLVVDEADGYAFLRQWGDDDYPEGHDPLPRLMRRTPMGYTATLLCV
                     LLRDKLRRFEEEEVHNERCVVETEELFEQWRAFFPASSDEVRRRKELGAALSKVEELG
                     FVTKFSADPEAWEVRRALKARLPVAELERLRDKLAAATAERVGGNGQ"
     gene            complement(3946..5466)
                     /locus_tag="VFD06_03110"
     CDS             complement(3946..5466)
                     /locus_tag="VFD06_03110"
                     /inference="COORDINATES: protein motif:HMM:NF023283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 family protein"
                     /protein_id="HZN02557.1"
                     /translation="MELDALKAYLDTSPALKLLKSPNAAYIIDFLYRTFKRSNRITVR
                     TSDLQSSLDDYREGLHETYPDALADKAENYLATWCSGETRWLHRFLEGGRNEPQFQLT
                     PHAEDVFVFLDRALQRDLGFVGTESRLKLVISMLADLVAEASSDADVRLKHLMEQRAR
                     IDAEIASVARDGVTTRLEPAAVRERFSTAVSLLKQLLADFRAVEERFKEITQQVQRRQ
                     ADGRDTRGTILGFALDSEEVLKEEDQGVSFYEFVRFILSPIQQERLRALIEQLGRIED
                     IASQADGLLTVRRMVPSLLAEAEKVMRTNQRLSATLRRLLDSRAASERRRLAQLLDQI
                     GGLAARLADAAPLDGTFAEPDGGVGLGSGLERAFWAHPSRFTKVDLIERVADEAERLK
                     TFRLLARMQRLDFRAMRARVARLTAATPGTVTLRQVVEAEPPVAGVVELLGYLQIAKD
                     DRHVVSRTASEEIVLRLPGRGGRVRRVTVPLVFFLPAHGTERGSNGQQQPSAARDV"
     gene            complement(5544..>6877)
                     /locus_tag="VFD06_03115"
     CDS             complement(5544..>6877)
                     /locus_tag="VFD06_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02558.1"
                     /translation="WTATAWPTAEDLKALAREAAKLTQARRELAQAQKVYDRWKELST
                     KAEFLAEALAARPAGLPADLGTLRDELEQARAEYAAAGAAATSRREELARQRAEHQRL
                     VERQQRLQSEIGPIEQRLGQEEVTQQHLEAGIEQNMTCLAPEWAASVAGSPTELLAER
                     LRGWQREAQDLEASGVEGDHEALGQAREQVERLDGRIAEWTADAERLPAEARRDPTEV
                     AGLLEEARKSRQQQEVAARDAERRREGLNERRRQREELQQRHRAADRRNRNYKLLAEL
                     LGRNGLQMELIRRAERAVVEYARHILDRLSGGDLMLQLRGRTDVDGTPADQALDLEVV
                     PVRSGAARPVPVAMLSGSQKFRVAVSLALAIGQYAGGRHRCGECVIIDEGFGALDKAG
                     QDVMIQELHNLKGLLKRIVVVSHQEAFAEAFSEGYRFKLENGQTRVERVGL"
BASE COUNT         1000 a         2519 c         2181 g         1177 t
ORIGIN      
        1 actgaagatc tcggacgtgt tcgcctgcgg atctttgacc acatggaggt aacagccgac
       61 gtacggctcg gtcacgcggg ccttggcgtc cagtggcgcc acgatcagga gctgcagtcc
      121 gaacttcgca aacagctcta gggcatactg cgcgtagagg tcgtcaacct tcgagaacat
      181 ctcgtcaacg accacgaacc cgaaccgctc gctcgacggg cggcccgggt cgatgtcgta
      241 ctggtacgcg atggcggcca ccaagatcgt gaacgcgagc ttcgccttct cgccgccgga
      301 cagccccgcg ctgtcctcgt aatacacccg ctcgacgtcc gtgccctcct ccatctcgcg
      361 agcggcgaag tcgaaccacc gccgcacgtc cgtgaccttc tcacgccaac gctgctcgtc
      421 gcgcagtcgt gccaggaact tgtcgatctt cacgaacctt gcctcgtcgg cctcgggcgt
      481 accctcgaag ctgccggaca agcactcgcg caaacccgcc tggaaatctg cgatctcgcg
      541 gtcacggacc ggacgcggct ccagtcggat gtacgtgccg ggtcggtact cgagctggcg
      601 gagtgaccgg ttaaggagct caatcttcga ctcgatctcg ccgcgctcgg tctggagcgc
      661 cccgtgcagc aggccaattt cctgggtcac cttctcgttc aggcgctcct tgaaccgggc
      721 ctcgtactgc ggcaggtcgt cccgccggac ctgctcacac aaggcgcaga agctgtcgag
      781 gtactcgacc cgcgcggcga ggtcggcgca gtcctcgggg aacttgttta ggtaatggtg
      841 catcccgtcg cacagcgcct gccgctcggg ctccagctcg gagcggagcc ggtcgatgag
      901 accccgttgc tgccggccga acccctgctc ctcctcgacc aacgtctcgg ccgacagcgg
      961 ccggtcggct aacagcgcgg cgatcccagc gaacgactca gcatgcgcgt cgagatcgcc
     1021 cgaggcccgg cgttcgtcca gaacgtgacg tgcgttagac agcgcacgct cgccctcgag
     1081 gaccgccctc gacaaccgat cctgtcgcac aacgcacgcg tcacgctccg gctggagtcg
     1141 tgcgaccgcg gcctcgtctt cggcaagccg ctgcttcaag aggcgtaaag cttggttgcc
     1201 cgcctccaga gcggccttct cacggtccag cgcctccgcc tgccgctcgt acgggcgggc
     1261 gtcgattgcc gcgaagtctg ccaccgcccc gagctgagcg agggcggcat ttcggtggcg
     1321 gagcgtctcc atggcggccg cgagtccctg cacgcgctcg tccagcgtcc gggcctgtcc
     1381 gcgcaagcgc accagcccct cggccagcag ccgccgcttc tctcggttgt cccacccgag
     1441 cacgaaccgg cgcgggtcga gcgcatggtc gcggtcgtcc ttgtcgtggc ggacctcgtt
     1501 ccgcttcacg tgccgctgtc gcgttacggc agatacgcgg gctgactgga actcctcaac
     1561 cgtctcgcag cactgatagt tgtgacgcgc ctccagctcc gcccgcaccc gcgccacgag
     1621 cggctgcccg tcgcgaaagt cgagcttgcg caccaaggac tgcgggtgca gtggcggtag
     1681 gttgcgcggc ggcgagctgg tctcgccgac gcgaaggtac gtcagccgct ggccgacccc
     1741 atggcggtcc tcgatccgcg tccggtccac gtagccactg accgtcgcgt agtaacgcgt
     1801 gggcacgagc agactgagcg cgaatccgcg cagcaccatc tcgatcgacg gctgccagtc
     1861 gcgctggtcg gggcggaccg cgaccaactc tgccacgaac ggcagttccc gctcgggcac
     1921 tccgacgccc tcgcacaacc gccttcgcgc ctcggccaac gccaccggca ggttcccctg
     1981 ccgctccgcc agcgccgcga gctccctctg gtcctcgtcc tcctgccggc gtactgcgtc
     2041 gcgatcgacg acggcgcggt tcctttcctc gtccagcgcg cgaagatcgc cggcgatccg
     2101 cgcggcggcc tctggcagcc gctggcgcag cgcggcaagc gcggccgcgt ccgggaccgc
     2161 gtcggtgatg cccgcggccc gcagcgcctc gtcgaaccgc cgtcgggccg cccgcgctgc
     2221 gtccgccccg tcccgcgcac gccgcagcag gtgcgggatc tcccgcagcc gctgcccgcc
     2281 ggactgctcg atctcgttgc gcgtcgtgcg gatggactcg gccacgtcgt ggagctcgga
     2341 gtcgatcgcg cagcggcgtg cggcgttctg ctccagctcg ccccggaggc gcgcgcattc
     2401 tggtacgaac agcgtgacgg tcttggtgcg gaagaacgtg tcggcggcgg ccagcatccg
     2461 ctccgccgcg gcgtactcct ccgcctttgc gcggtaggtc ttccccttcg cctcgacggg
     2521 caacagcagc tcgaactgct ggcgcacgcg gactaggctg cggtgagcct cgctcagctg
     2581 cgtgaagtgg ctctgcagcg catcgatccg ttcgccccag ggcttcgcct cgagcatgtg
     2641 ctcgcggatg aaccggttca ggctctggat gtccttgacg gcgaccgtct ggttgaacat
     2701 gtccatcgcc ttcgcccgca ggccggtcgc gcgggcgaac cacttgtaat attccgtgta
     2761 gctggtggtc gcgcggaagc cgcggcgctc catctgctgc ctgagcttcc ctgtgtcggg
     2821 caggttcgcg caatgctccg cgatcgaccg ctcctcgtcc gagaagcagt agaccttctg
     2881 gggttggccc tcgccgtcca ggtagagcac ctgcgccacc gtgaacgccg cgccagcctc
     2941 gttccggaag caggccagca gggcggagta gtgcaaccgg tcgccccgta gatattggat
     3001 ttccgcgcgg ttgtcgtcgt cccgcccgag gcggccgcac gcccccctaa tgtaagaccg
     3061 ctcgtcccgc tcctgcttct gcgccccggc ggccacgttg tagttgcgca cgaccgggcg
     3121 gacgagcagc gtcaggatcg cgtcgaccag cgttgacttg ccggagccgt tctggccgat
     3181 tagcagcgtc gtcgccccgt cggggttcac tgtgtgaatg tggccgcgcg agctgtcgaa
     3241 cgtgccccag ttgaacaact ccagcttcgc cagccgaaag ccgtgccagc caacgggggg
     3301 cggaacgggc actgcggcgg gcgaagcgac ggccgaagcc tcgaccaaat gtgcgtcgtt
     3361 cattgcccgt tccccccgac acgctcggcc gtggccgccg ccagcttgtc ccggagccgc
     3421 tccagctccg cgacgggcaa ccgcgccttc agggcccggc ggacctccca cgcctccggg
     3481 tccgcactga acttcgtcac gaaccccagc tcctcgacct tcgagagtgc ggcgccgagt
     3541 tccttgcggc ggcggacctc gtcggacgat gccgggaaga aggcccgcca ctgctcgaac
     3601 agctcctcgg tctcgacgac gcaccgttcg ttgtggacct cctcttcctc gaaccgccgc
     3661 agcttgtcgc ggagcagcac gcaaagcagc gtcgctgtat atcccatcgg cgtccgccgc
     3721 atgagccgcg gcagcgggtc gtggccctcg gggtagtcgt cgtcgcccca ctgcctgaga
     3781 aacgcataac cgtccgcctc gtcgacgacg agtgagaggc cgattcgacc gaagtacgaa
     3841 tccagtgccg atttcgagcg gagcagcacg tcccagacct tcggctcgtc gtcgtagacg
     3901 acgccctgaa gaagcttgac cgccgcgaca ctccactccc ggagctcaga cgtcgcgtgc
     3961 tgcgctgggc tgttgctggc cattgctgcc cctttctgtg ccgtgagcgg gcaagaagaa
     4021 caccagcggc acggttacgc gccgcacccg accgccgcgc cccggcaggc ggagaacgat
     4081 ctcttccgac gccgtgcggc tgacgacgtg cctgtcgtcc ttggcgatct gcagatagcc
     4141 gaggagctcc accactccgg cgacgggcgg ctcggcctcg acgacttgcc gcagcgtcac
     4201 ggttccgggc gtcgccgccg tcagccgcgc gaccctggcc cgcatcgccc ggaagtcgag
     4261 gcgctgcatc cgggccagca gccggaaagt cttcagccgc tctgcttcgt cggcgacgcg
     4321 ctcaatcagg tccaccttgg tgaatcgaga cgggtgggcc caaaaggccc gttccaagcc
     4381 actcccgagc ccgacgcccc cgtccggctc tgcgaaggtg ccgtcgaggg gggcggcgtc
     4441 cgccagacgg gcggccagcc ccccgatctg gtcgagcagc tgggcgaggc ggcggcgttc
     4501 cgacgccgcg cgcgagtcga gcagcctgcg gagcgtggcc gacagccgct ggttggtgcg
     4561 cataaccttt tcggcctccg ccaggagcga cggcaccatc cgccgcaccg tgagcagtcc
     4621 gtcggcctgg gaggcgatgt cttcgatccg cccgagctgc tcgatcaggg cgcgcagccg
     4681 ctcctgctgg atgggcgaga ggatgaagcg cacgaactcg tagaagctca ccccctggtc
     4741 ctcctccttc aggacctcct cgctgtccag cgcaaagccc aagatcgtgc cgcgggtgtc
     4801 ccgcccgtcc gcctgacgcc gctggacctg ctgcgtgatc tccttgaacc gctcctccac
     4861 cgcgcggaag tccgcgagca gctgcttgag cagcgagacc gccgtcgaga aacgctcccg
     4921 cacggccgcc ggctctagcc gcgtcgtcac gccgtcccgc gcgacactgg cgatctctgc
     4981 atcgatgcgc gcccgctgct ccatcaggtg cttgagccgc acgtccgcgt cgctcgacgc
     5041 ctccgcaacg aggtcggcca acatgctgat gaccaatttg agccgcgatt cggtgccgac
     5101 gaatccgagg tcgcgctgca gcgcgcggtc aaggaataca aagacgtcct ccgcgtgcgg
     5161 cgtaagctgg aactgcggct cgttgcgccc gccctccagg aaccggtgca gccaccgggt
     5221 ctcgcccgag caccaagtgg ccaagtagtt ctccgccttg tcggccagcg cgtccgggta
     5281 cgtctcgtgg aggccctccc ggtagtcgtc gagggatgac tggaggtcgg acgtgcgcac
     5341 ggtaatccgg ttcgaccttt taaaggtgcg gtacaggaag tcgatgatgt acgccgcgtt
     5401 aggcgatttc agcagcttca gcgccggcga cgtgtccagg taggctttga gcgcgtccag
     5461 ttccaacggt tacctctcgt acagacagca tttcgcatta ggcatcccgg ccgaacctcg
     5521 cgctgagcat gcgacggtcg ctgttacaac ccgacccgct ccacccgagt ttgcccgttc
     5581 tccagcttga accggtaccc ttccgaaaac gcttcggcga atgcttcttg gtggcttacc
     5641 acgacgatgc gcttgagcag ccccttgagg ttgtgcagct cctggatcat cacgtcctgc
     5701 ccggccttgt cgagcgctcc gaagccctcg tcgatgatga cgcactcgcc gcagcgatgg
     5761 cggccgccgg cgtactggcc gatcgccagc gctaaactca cggccacccg gaacttctgg
     5821 ctgccgctga gcattgccac cggcactggg cgggcggcgc cgctgcggac tggaaccacc
     5881 tccaggtcca gtgcctggtc ggccggggtg ccgtcgacat ccgtccggcc gcgcagttgg
     5941 agcatcaggt cccctccgga gaggcggtct aggatgtgcc gggcgtattc gacgacggcg
     6001 cgctcggccc ggcggatcag ttccatctgc aagccattac ggcccagcag ctcggcgagc
     6061 aacttgtagt tccggttcct gcggtcggct gcacggtggc gctgctgcaa ctcctcgcgc
     6121 tggcggcggc gctcatttaa cccctctcgc cgccgctcgg cgtcccgcgc ggccacctcc
     6181 tgctgttgcc gggatttccg cgcctcttcc agtaggcccg cgacttccgt cggatcccgc
     6241 cgcgcctccg ctggtagtcg ctcggcgtcg gccgtccatt ccgcgatccg tccgtccagc
     6301 cgctctacct gttcccgggc ctgccccagc gcctcgtgat ctccctccac ccccgacgcc
     6361 tccagatcct gagcctcgcg ctgccagccc cgtagacgct ccgccaacaa ctcggtcggc
     6421 gaaccagcga cagatgccgc ccactcggga gccaagcatg tcatgttctg ctcgatcccg
     6481 gcttcaaggt gctgctgggt aacctcttct tgcccgagcc gctgttcaat cgggccaatc
     6541 tcggactgaa gccgctgttg gcgctcgacc agtcgctggt gctccgcgcg ctggcgggcc
     6601 aactcctcgc ggcgcgatgt ggccgccgct ccggcggcgg cgtactccgc cctggcctgc
     6661 tccagctcgt cccgaagtgt tcccagatcg gccggcaggc ccgccgggcg cgccgccagg
     6721 gcttcggcga ggaattcggc cttggtggac agttctttcc agcggtcgta gaccttctgg
     6781 gcctgcgcca gctcccggcg agcctgggtc agcttcgccg cctcgcgcgc caacgccttc
     6841 aagtcctccg cggtcggcca ggcggtggcg gtccatt
//
LOCUS       DASRIQ010000132         9209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_315345, whole genome shotgun sequence.
ACCESSION   DASRIQ010000132 DASRIQ010000000
VERSION     DASRIQ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315345"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            922..1926
                     /locus_tag="VFD06_03120"
     CDS             922..1926
                     /locus_tag="VFD06_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02559.1"
                     /translation="MTTLTVQANDARSGLKTMWGEIRSPNRSAALSFGSGNVSGGTVF
                     SFPISLPRAAQTGTWYVAWLSLTDQAGNTKLIQAASASAAPPGGTFAAFSSESDATPP
                     EVLQVWFDRSAVGPGEKNTIAVEARDDRSGVASVIGACQSPSKSALIWFSGALNADSG
                     TWVGEVSIPKTADCGEWVVQQLAVKDKAGNTTLLQANSPPLARAGFRVASGSDCDFTA
                     PTLDAFDLSPAIVLSGTATEILVTARVYDEGSGAATMTGWFEGPAPAGGQSPKNPLSC
                     SPDPRDPDAPWTCRIQVPQLAAKGTWKVGAIRLEDKAKNVRTYTSADPVVSGRVFQVQ
                     "
     gene            2096..4453
                     /locus_tag="VFD06_03125"
     CDS             2096..4453
                     /locus_tag="VFD06_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02560.1"
                     /translation="MMLLVGVVFAGSAFAATGTGARELDVEKRISAQAAIERVYWSHR
                     IWPAGNDGPKPVLHDVLPERALRASVENDLLQSKALEQLWDRPIRDAELQAEIERMVA
                     SSRAPGVLQELFAALGNDPILVAESLVRPILTDRLIRNAYAYDARFHQEQRTAIEQSL
                     SRRPTISDLRREAEYAEAIWVRGGAQPERAARRAGARLIPMSDETWQRSLSRLQEGFE
                     PGSTGPQIHPGFENLPVGILSGLQEDDESFFVQAVLEKSDARLKVATVAWRKKPFDEW
                     WAGASGEIAPGSPDLGAAIGSGASLDALPVIAAASCTSNTWAAGQTTGAPSPRQAFSS
                     VWTGTEMIVWGGYNGTSNLDTNTGSRYNPATNSWTATPTTGAPQLRDSHSAVWTGAKM
                     VVWGGGNELYVPKDTGGRYDPATNTWQATTTTGAPTARLFHTTVWSGSRMIVWGGWNG
                     TNRDFDTGGLYDPAADAWTATSRTGAPTARDTHTAVWSGTRMIVWGGEDDVPVAQNTG
                     ARFDPVANSWTAMSLTDVPAARWMHTAVWTGTRMIVWGGFDGVTDLNTGALYDPAANT
                     WTATSTTGAPAGREMHVAVWADAVSEMIVWGGQDDTVVPLNTGGRYNPGTGVWAATST
                     VGAPTWGRYGGAAWTGSEMIVWGGWNVTGQVDLNTGGRYCVGACAAAPPGGSSSIAVS
                     NQGNGDLVSWSAVAGADAYDLVRGSLSLLNSSGGNFTTSLQACLADDQAGLGFVDTAL
                     PPVGNGFWYLVRGLSCGGAGTYDSVGGNQVGSRDAEIAASPGSCP"
     gene            4478..4768
                     /locus_tag="VFD06_03130"
     CDS             4478..4768
                     /locus_tag="VFD06_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02561.1"
                     /translation="MKSSWPKAGLAAAALTLALATPGRAQEKSNDDLMKEMESLRQML
                     QAIQKDLQEVKGMLARQAPAPSGIGVVLDLGKAPVKGEKTAKLTLVEFSDYQ"
     gene            4781..5749
                     /locus_tag="VFD06_03135"
                     /pseudo
     CDS             4781..5749
                     /locus_tag="VFD06_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            5781..8123
                     /locus_tag="VFD06_03140"
     CDS             5781..8123
                     /locus_tag="VFD06_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HZN02562.1"
                     /translation="MNRLPALLGIGLAFAGIVGAVPAARDREAPPPRVTALLLNGGGT
                     PSANYQSHLSHLRLLVKVLGEAGVPARRITILSGDGPNPAADVALREAQPEEDFWLLG
                     GTRLEAPFRTPITFENTSLPGYALKAATMTEIDRWFGSVGSRLTEGDTLLLYVTDHGT
                     KNTADTRDNRITLWGKNQGLSVGALRARLAKLDRRVRVVMLMSQCYSGSFANAAPREA
                     SGLPSGNVCGYFSTTAERPAYGCYPEVRDKENTGHSFDFIEELAQAGRFPEAHTQVLR
                     SDATPDAPLRTSDAFLEELLRKKAESSGAGYDAFVDALLARAWKRKDAWEPEIRLLDA
                     IGHNFGTFSPRSLAEIREQEARLPEVSEQLDTHAKAWQETLGDANRANLDRFLARRPA
                     WGDLLNDRALRNGAARELSGSFVYDLADVTRSDAAMAKRLFTLRDKADTARALAYRME
                     VREGVMLRMRTILATVAGREYLARDGAREERAAYEALLECESFRLPLARPVPGMLPTD
                     RAPFPAMEDDVAAALAVLPAWLGIRFGDVPASLRKAHQLAPGASSVQAVFPDSPAAAA
                     DLQAGDVILGPPAARFEDPRQIREWTMLATVDEPAVLTLLRGDETIEKTIVPKPYPMK
                     WPGLPGPPRVSTAAPAWKPLQLAAYRGALPSELRGAGAHLLFFWATWCGPCKASLPEL
                     LAFESERKIPVVAITDETGETLDAFFGGFSQPFPERVLTDEQRRSFLAYGVSGTPTFV
                     LVDGEGVIRAYSVGYTRATGLPIEGWTWSGRSAIAPRVAP"
     gene            8363..>9209
                     /locus_tag="VFD06_03145"
     CDS             8363..>9209
                     /locus_tag="VFD06_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02563.1"
                     /translation="MTSLMTVPHESGALAQSPVASRPEKIVLAPKHPEGRGRGRIAIA
                     PDAAAWHRLFNAGSARVADFPLPGGHRIDLEVSSVDIVGGRTRFFVNGGEGVREVAGP
                     AMRFFRGRVAGDPESLVSLNLFGGRIAGFVRHDGREYTFGPRAFSVDRGGADDIEVVD
                     ETMEEGPTGGCDGDETADSGGVFALTGRPDLERTYSPNIDAGTLLLGWIAVEGTVEWV
                     TKHGGVAAATTYTLNLMAQVSAIYESDIKVQLQVPYILMNAAEPDGYSGASNSTSTVL
                     NEMRAK"
BASE COUNT         1538 a         3055 c         3234 g         1382 t
ORIGIN      
        1 cgagcacctg gaagccccgt ggaccgggaa ggtcctggtc ccgcagttcg cccccaaggg
       61 gccgtggaag gtgcgcgtga tccgcctgga agacaaggcg aagaacgcgc gtgagtacac
      121 ccccagcgac ccggtcctgt ccggaggcgc cttcgaggtc cgatgagccg cgtctcgtcg
      181 gatccgcctc gaaagatcgt ccgcctgtgg atcggccgtc tgttgatcgc agccgtcgtc
      241 gtggaatgcg tggtgctcgg tgcgatccac ctccggcgat ggatcgggaa cgacgaccgg
      301 atcggcgcgt ccggagcgtc ggagccggac gaggagtcgc tcgccgggcc gtcgccgtca
      361 cctcccgcgc catcgccaca ggtggcgcag acgcggggac cgggggcgat gggtgagcac
      421 ccgcaatcga cggagggtcg atcggatccc gatggagacg gtcggatggg gacgcaagca
      481 gccggcgaca ttcggcgcag cgaatccaat cagacggcgc cggtggggga cggcccggtc
      541 ggcggaacag gctcgtcggg cggcatggat ccgacgaagg atcctgcagg agccgcgaac
      601 gttgccgggc ccggcatgaa cgcgcgcccg ctcaccgcgc ccgccggcgc gcctccaggc
      661 ggatcctcga cgccgtcttc cagccggccc agatcgtcgg gaggcgccgt gggcccgtcc
      721 gtgtcgctgc ccgtgccggg aatgccggtt gcggagggtg acgcgggctc ctcgggagac
      781 ccgaagccag atgaggacac ccaggaggag catgacaagt cagacgagga tgattctgga
      841 ggggacgatt ccggagcgga cagcacaccg ccggttctcg agtcgctgcg cttcgacccc
      901 ccgcaaatcg aaggcggcaa cgtgacgacg ctgaccgttc aggcgaacga tgcccggtcg
      961 gggctgaaga ccatgtgggg ggagattcgc agccccaaca ggtcggccgc cctgagcttc
     1021 gggtcgggga atgtgagtgg cggcacggtg ttcagctttc cgatttcgct tccgcgcgcg
     1081 gcccagaccg ggacatggta cgtggcgtgg ctctctctga ccgaccaggc gggcaacacg
     1141 aaactgatcc aggcagcttc agcctcggcg gcccctcccg ggggcacgtt cgcggcgttc
     1201 tcctcggagt ccgacgcaac ccctccggag gtcctccagg tctggttcga caggagtgcg
     1261 gtcggcccgg gggagaagaa caccatcgcg gtggaggcgc gagacgaccg atcgggggtg
     1321 gcctccgtga ttggagcgtg ccagagcccg tccaagtccg cgctcatctg gttcagcggt
     1381 gctctgaatg ccgattcggg aacgtgggtg ggggaggtct ccattcctaa aaccgccgat
     1441 tgcggggaat gggtcgtgca gcagctcgcc gtcaaggaca aggcggggaa caccacgctt
     1501 ctccaggcga attcgcctcc cctggctcgg gcagggttcc gcgtcgcctc cgggtccgac
     1561 tgcgacttca ccgccccgac cctggatgca ttcgacctgt ccccggcgat cgtcctgagc
     1621 gggacggcga ccgaaatcct cgtcaccgcg agggtctacg acgaggggag cggagccgcc
     1681 acgatgacag ggtggttcga gggacccgcc ccggcaggcg gccagtcccc caagaacccc
     1741 ctaagttgtt cacccgaccc ccgggatccc gatgcgccgt ggacatgcag aatccaggtc
     1801 ccgcagctcg ccgccaaggg gacctggaag gtcggcgcga ttcgcctcga ggacaaggcc
     1861 aagaacgtcc gtacctatac ctccgcggac ccggtcgttt cgggacgcgt ctttcaggtt
     1921 caatgaatag aaaaaactca ccacatataa aggagaaaaa actcagcaca caatgattgg
     1981 ataggctcta taatccgccg ttctcagccc gccgcgcgca ttccaacaac ttaggcttcg
     2041 atctcgaggg gccggtccta gaccgagccc ggggggaatc atgtcaaggt cagggatgat
     2101 gctcttggtt ggggtggtgt tcgctggcag tgcattcgcg gccactggga cgggcgcgcg
     2161 cgagctcgac gtcgaaaaac gcatttccgc ccaggcggcc atcgaacgtg tctactggag
     2221 ccatcggatc tggccggccg gcaatgacgg cccgaagccg gtgctgcatg acgtgctgcc
     2281 ggaacgcgcg ctgcgcgcat cggtcgaaaa cgacctgctc cagtcgaagg ccctcgagca
     2341 gctctgggac cgtccgatcc gcgacgcgga gcttcaggcc gagatcgaga gaatggtcgc
     2401 gtccagtcgc gcgcccggcg tccttcagga actgtttgcg gcgctcggga acgacccgat
     2461 cctcgtggcc gagagcctag tgcggcccat cctgacggac cggctgattc gcaatgccta
     2521 cgcctatgac gcacgctttc atcaggagca gaggaccgcg atcgagcaat ccctgtcgcg
     2581 gcggccgacc atctccgacc tgcgcaggga agcggaatac gccgaagcca tctgggtgcg
     2641 cggcggcgcg cagccggagc gagccgcaag gcgcgccggt gcgcgtttga ttccgatgag
     2701 cgacgaaacc tggcagcgca gcctgtcacg gcttcaggag gggttcgagc ccggttccac
     2761 cgggccgcag atccatccgg ggttcgagaa tctgccggtc gggatcctga gcggccttca
     2821 ggaggatgac gagagtttct ttgtgcaggc cgtcctcgag aaaagcgacg cgcgcctcaa
     2881 ggtggcgacc gtggcctggc gcaagaaacc tttcgatgag tggtgggccg gagcgagcgg
     2941 ggagatcgcc cccggctctc ccgacctcgg cgccgcgatc gggagcggcg catcgctcga
     3001 tgcgctgccg gtgatcgcag cggcgtcttg cacatccaat acctgggcgg ccggccagac
     3061 caccggcgcc ccctcccccc gccaggcgtt cagctccgtc tggaccggca ctgagatgat
     3121 cgtctggggc ggctacaacg ggacctcgaa cctggacacc aacaccggca gccgctacaa
     3181 cccggcgacg aatagctgga ctgccacgcc gacgaccggc gcaccccaat tgcgtgacag
     3241 tcactccgcg gtctggaccg gcgcgaagat ggtggtgtgg ggtggcggca acgagttgta
     3301 cgtcccgaaa gataccggtg gacgatacga tccggcgacg aacacctggc aggcgaccac
     3361 gacgacgggc gcacccaccg cccgcctctt ccacacgact gtgtggtcgg gctccagaat
     3421 gatcgtctgg ggcggatgga acgggacgaa ccgggacttc gatacgggag ggctttacga
     3481 tccggcggcg gatgcgtgga cggcgacgtc ccgaacggga gctcccaccg cccgagacac
     3541 gcacaccgcc gtctggagcg ggacgaggat gatcgtgtgg ggtggtgagg acgacgtccc
     3601 cgtcgcccag aacaccggcg cccgtttcga tccggtcgcc aactcctgga cggcgatgtc
     3661 cctcacggac gtccccgccg cgcggtggat gcacaccgcc gtgtggaccg gaacccggat
     3721 gatcgtctgg ggcggcttcg acggtgtcac ggacctcaac accggagctc tctacgatcc
     3781 cgcagcgaat acctggactg cgacttcgac caccggagcg ccagcaggcc gggagatgca
     3841 tgtcgccgtc tgggccgacg cggtgtcgga gatgatcgtc tggggcggcc aggacgacac
     3901 cgtcgtcccg ttgaacaccg gcggacgata caatccgggg accggggtct gggccgccac
     3961 gtccaccgtc ggggctccca cgtgggggag gtacggcggc gcggcttgga ccggctcgga
     4021 gatgatcgtg tggggcgggt ggaacgtgac gggccaggtg gatctgaaca cgggcggacg
     4081 ctactgcgtc ggggcctgcg ccgccgctcc gcccggggga tcctcgagca tcgccgtcag
     4141 caaccagggg aatggagacc tcgtttcctg gtcggcggtc gcgggggccg atgcgtacga
     4201 cctggtgcgc gggtcgctca gcctgctcaa ctcgtcgggg ggcaacttca cgacgtccct
     4261 gcaagcctgc ctggcggacg atcaggcggg gttgggtttc gtcgataccg ccctgcctcc
     4321 cgtggggaac ggattctggt acctggtgcg cggtctgagc tgcggcggcg cgggcaccta
     4381 cgactcggtc ggcgggaatc aggtcggaag ccgcgatgcg gagatcgccg cctcgcccgg
     4441 cagctgcccc tgaaacgttg aagagggagg agaaccgatg aagtcatcgt ggccgaaggc
     4501 gggcctggca gcggcggcgc tgacgttggc gctggcgacg ccgggccgtg cccaggagaa
     4561 atcgaatgat gacctcatga aggagatgga atccctgcgg cagatgctgc aggcgattca
     4621 gaaagacctt caggaggtca aagggatgct ggcgcggcag gcacccgctc cgtccggcat
     4681 cggagtcgtc ctcgacctgg ggaaggcgcc cgtcaaaggg gagaagacgg cgaagctgac
     4741 cctcgtcgaa ttctccgact accagtgacc cttctgcgcc aagtacgtcc gtgagacgta
     4801 cccgcagatc gacagcgaat acatcaagac cggcaagctg agaacggtgt tcatggacat
     4861 gccgctcgag agcattcaca agttcgcgtt caaggccgcg caggcgacga gttgcgcggg
     4921 agtggaaggg aagttctggg agatgcacga tcggctcttc gaaaaccaga aggccctcga
     4981 gccatggacg gcgcacgctc aggcggttgg aatcgacgtt ccgaagttcg aggcctgcct
     5041 cgccagcgga aaatttgaca cggagatccg ccggcagatg ggcgaggcca agaaggccgg
     5101 cgtcacgggc acccccgctt tcctgatcgg ccggacggag cccaacagca cccaggtgaa
     5161 gatcctggcc gtgctcaagg gggccaaggc gttcgcggac ttcaagacgg agctggatcg
     5221 cctgctggcg gaggcggaga agtccccctc ggcggccgct cccgccggcg cgccgacgcc
     5281 ggagaaaatc gcggagtccg gatcgaaggt cgcggatgcg ccggcacgcg ccgccatcgc
     5341 tcgcgacgga aagctggacg acacgacgcg cgcgagcctg gagcgcgtgc tgcggacggc
     5401 cccggccgga tcgcccgcct ggatcgcggc cccgaaatcg gatgcgaggg ccgtcaccct
     5461 ggcgaaggag atctcggggg ccttcacggg cgcgggatgg cgggtgaggc cggtgcggca
     5521 gacgggggtc aggccccgtc ccggcacgta cgtgttcgca gccgacgagg agcctccccc
     5581 gtacgtggag acggtcgctc aggctctcga agaggcgggt ctcgacgcga aggtcctcac
     5641 cggctatcgc gcgtactacg acgagaaaat gaaggccgat ccgaagttcc agggctatcc
     5701 gttcgccccc gagcagacct tcctggtggt gatcggcccc atgccatgag acggcggcgg
     5761 ttcccgcggc cggcgtccgc ttgaatcgcc ttcccgcgct cctcggcatc ggtctcgcgt
     5821 tcgccgggat cgtcggcgcg gtgcccgccg cccgcgaccg cgaagcccct cctccgcgcg
     5881 tcaccgccct gctcctcaat ggcgggggga cgccctccgc gaattaccag tcacacctga
     5941 gccatctccg cctgcttgtg aaggtgctag gcgaggcggg tgttccggcg cgccgcatca
     6001 ccatcctgtc gggggacggg ccgaaccccg ccgcggatgt tgccttgagg gaggcgcagc
     6061 cggaggagga tttctggctg ctcggcggaa cccggctgga ggccccgttc cggaccccca
     6121 tcaccttcga gaacacgagc ctgccggggt acgccctcaa agcggccacc atgacggaga
     6181 tcgatcgctg gttcggctcc gtcgggtcgc ggctgaccga gggggacacg ctgctgctct
     6241 acgtcaccga ccacggcacg aagaacaccg cggacacccg cgacaaccgg atcacgctgt
     6301 ggggaaagaa ccagggcctg tcggtgggcg cgctgcgcgc ccgcctggcg aagctcgacc
     6361 ggcgtgtccg cgtcgtcatg ctgatgtcgc agtgttactc gggctcgttc gcgaacgccg
     6421 cgccgcgcga ggcgtccggt cttccgtccg ggaatgtctg cggctacttc tcgaccacgg
     6481 ccgaaaggcc cgcctacggc tgctacccgg aggtccgcga caaagagaac acgggacact
     6541 ctttcgattt catcgaagag ctggcccagg ccggccgatt tcccgaggcg cacacgcagg
     6601 tgctccgcag cgacgccacg cccgacgccc cgctgcgcac ctcggacgcg ttcctcgaag
     6661 agctgctgcg gaagaaagcc gagtcgtcgg gcgccgggta cgacgcgttc gtcgatgcgc
     6721 tgctggcccg ggcctggaaa cggaaagacg cctgggagcc ggagattcgc ctgctcgacg
     6781 ccatcggcca caacttcggc acgttcagcc cccgctcgct cgctgagatc cgggagcagg
     6841 aagcccgcct ccccgaggtc agcgaacagc tcgacacgca cgcgaaggcc tggcaggaga
     6901 cgctcggcga cgccaaccgc gcgaacctcg atcgcttcct cgcccggcga ccggcgtggg
     6961 gcgatctgct gaacgaccgc gccctgcgca acggcgcggc ccgcgagctg agcggctcct
     7021 tcgtctatga cctcgccgac gtcacgcgct cggacgccgc gatggcgaag cgactcttca
     7081 cgctgcggga caaggccgac acggcgcggg cgctggcgta tcgcatggag gtgcgcgagg
     7141 gggtgatgct gcggatgcgg acgatcctcg ccacggtcgc aggccgcgaa tacctggcgc
     7201 gcgacggcgc acgcgaagag cgcgccgcct acgaggcgct cctggagtgc gagtcgttcc
     7261 gtctgcccct cgcccgaccc gttccgggca tgctgccgac ggatcgggcc ccgttccccg
     7321 cgatggagga tgacgtggcc gcggcgctcg ccgtgcttcc ggcgtggctc ggcatccgct
     7381 tcggcgatgt cccggcctcc ctgcgcaagg cgcatcaact ggcgccgggg gcgtcgtcgg
     7441 tgcaggccgt gttcccggac tcaccggccg cggcggccga cctccaggcg ggggacgtga
     7501 tcctcgggcc gcccgcggcc cggttcgagg atccccggca gatccgcgag tggacgatgc
     7561 tcgccaccgt ggacgagccg gccgtgctca ccctgctgcg cggcgatgag acgatcgaga
     7621 agaccatcgt cccgaagccg tatcctatga agtggcccgg gctccccggg ccgccccgcg
     7681 tctcgaccgc ggccccggcg tggaagcctc tgcagctggc ggcgtatcgc ggcgctttgc
     7741 cttccgaact gcgcggcgcc ggcgcgcatc tgctgttctt ctgggccacg tggtgcggcc
     7801 cctgcaaggc gtccctcccc gagctcctgg cgttcgagag cgaacgcaag atccccgttg
     7861 tggcgatcac tgacgagacc ggcgagacgc tcgacgcatt cttcggcgga ttctcccagc
     7921 cgttccccga gcgcgtgctg accgacgagc agcggcggtc gtttctggcc tacggcgtca
     7981 gcggaacgcc gacgttcgtc ctggtggacg gggagggcgt catccgcgcg tactccgtcg
     8041 gatacacgcg cgcgaccggc ctcccgatcg agggctggac ctggtcagga aggtccgcga
     8101 tcgccccccg cgtcgccccg tagccgcgcc gtccagattt gaaattctcc gcgacgggca
     8161 cggtttttat gccatccgat ggcctccacg atcgtaagcg ccggagctta cggaaacgat
     8221 ccctcccagc ccgtgacaac ggacatggcc ggccccaggt tctcctcaaa gccattgcag
     8281 gtgcgagggg ggggccatgg atcgtcttcc gtttgtcacg cgcaggaggt cctggcgggg
     8341 tcttgtcggg gctgccgcgt tcttgacgag tctgatgacc gtgccgcacg agagcggcgc
     8401 gctcgcgcag tcgccggtgg cttccagacc agagaagatc gtgctcgcgc cgaagcaccc
     8461 ggaaggcagg ggccggggcc ggatcgcgat cgctcccgac gccgcggctt ggcaccggct
     8521 cttcaacgcc ggcagcgccc gggtcgctga tttcccgctg cccggcgggc atcgtatcga
     8581 cctcgaggtc tcttcggtcg acatcgttgg aggaaggacc cgctttttcg tcaacggcgg
     8641 ggagggcgtc cgggaagtgg ccgggccggc gatgcggttc tttcgcggcc gcgtggcggg
     8701 ggatccggag agcttggtct cgctgaacct gttcggcggg cggatcgccg gcttcgtgcg
     8761 ccatgacggc cgcgagtaca cgtttggacc gcgcgccttc tcggtcgacc gggggggcgc
     8821 cgacgatatc gaagtggtgg acgagaccat ggaggagggt cccacgggcg gctgcgacgg
     8881 cgacgagacg gccgacagcg gcggcgtctt cgcgctcacc gggcgaccgg atctggagcg
     8941 gacctactcg cccaacatcg acgcggggac cctgctgctc gggtggatcg ccgtggaggg
     9001 cacggtcgag tgggtcacga agcacggcgg ggtggcggcg gcgacgacct acaccctgaa
     9061 cctgatggcg caggtctccg cgatctacga gagcgacatc aaggtgcagc tccaggttcc
     9121 gtacatcctg atgaacgccg ccgagccgga cggctactcg ggcgcatcga acagcaccag
     9181 cacggtcctg aacgagatgc gcgccaaat
//
LOCUS       DASRIQ010000133         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1358060, whole genome shotgun sequence.
ACCESSION   DASRIQ010000133 DASRIQ010000000
VERSION     DASRIQ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358060"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFD06_03150"
     CDS             complement(<1..872)
                     /locus_tag="VFD06_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate CoA-transferase"
                     /protein_id="HZN02564.1"
                     /translation="MVTFGSDAPRHLSQMCDEVMDVSWETRLRDKTMTADEALGAVQS
                     GGRVFIHGAAATPTPLVEALARRLPELSGVETVSIHTVGDAPYAALEHAAHARHRALF
                     IGQNVRAAVNAGRADYVPIFLSDIPPLFRNGAMPIDVALINVAPPDQHGYCSLGTSVD
                     VAKAAAEHARIVIAQLNPSMPRTLGDSFIHADQLTYAVEADLPPHAAPPEPISDEERR
                     IGEHIATLVEDGSTVQLGIGAIPNAAAAALRGRRDLGVHTELFSDGVVDLVEAGAITG
                     ARKNLHRGKIVSSFV"
     gene            973..1203
                     /locus_tag="VFD06_03155"
     CDS             973..1203
                     /locus_tag="VFD06_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02565.1"
                     /translation="MLDVIGAGPLIGPWLGVWDLVLDSPEASIALAAALFAMATTGLG
                     LVVLGGRRHQEPVETVAVVPARRPPRRMPRRR"
     gene            1447..2001
                     /locus_tag="VFD06_03160"
     CDS             1447..2001
                     /locus_tag="VFD06_03160"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016879686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZN02566.1"
                     /translation="MQLETIFTGRLVRLAAPRPDDHETFARWSENDQYLRTMDNDPAR
                     PISAEAHAAWEAPFLQSPTSFIFRIRTLADDALIGVVALGDVQWMHQCAMLGIAIGDP
                     ACWGKGYGSDAIDLMLRYAFLELNLYRVWLTTIAYNERARRAFEKAGFRPEGAWREMI
                     HRDGKRFDVLHYGLLRHEWEAHRT"
     gene            2167..>2293
                     /locus_tag="VFD06_03165"
     CDS             2167..>2293
                     /locus_tag="VFD06_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZN02567.1"
                     /translation="MDDLDQQLVELLAQRAEISREVGRRKGTQGGAVFAPARESEI"
BASE COUNT          323 a          819 c          798 g          353 t
ORIGIN      
        1 acgaacgagc tgacgatctt gccccggtgc aggttcttgc gcgccccggt gatggctccc
       61 gcctccacca ggtcgaccac gccgtcggag aacagctcgg tgtggacgcc gaggtcgcga
      121 cggccgcgga gggccgccgc cgccgcgttc ggaatggcgc cgatgccgag ctggacggtc
      181 gagccgtcct ccacgagcgt cgcgatgtgc tcgccgatcc ggcgctcctc gtcggagatc
      241 ggctccggcg gcgccgcgtg cggcggcaga tccgcctcga ccgcgtaggt aagctggtcg
      301 gcgtggatga agctgtcgcc aagggtgcgc ggcatcgacg ggttgagctg ggcaatcacg
      361 atccgagcgt gctcggcggc agccttcgcg acgtcgacgg atgtccccag cgagcagtag
      421 ccgtgctgat ccggcggcgc gacgttgatg agcgcgacgt cgatcggcat ggcgccgttc
      481 cggaacagcg gcgggatgtc ggagaggaag atcggcacgt agtcggcgcg ccccgcgttc
      541 accgctgccc gcacgttttg gccgatgaag agggcacggt gccgggcatg cgcggcgtgc
      601 tcgagcgccg cgtacggggc atcgccgacg gtgtggatcg agacggtctc cacgcctgac
      661 agctccggca ggcgccgagc cagcgcctcg acgagcggcg tcggcgtcgc ggctgccccg
      721 tggatgaaga cccggccgcc gctctggacg gccccgagcg cctcgtcggc cgtcatcgtc
      781 ttgtcgcgca gtcgcgtctc ccagctcaca tccatcacct catcgcacat ctgagacagg
      841 tgtcgcggcg cgtcactccc gaacgtgacc aaccgttctg cacgtcaaag gccgcgtgct
      901 cctcggagac ggcacgggca gcgtggcggg aggcgccgcg cgtcggagcg acccgcacag
      961 ggaggatagg acatgctcga cgtgatcggg gccgggccac tcatcgggcc gtggctcggg
     1021 gtctgggatc tggtcctcga ttcgccggag gccagcatcg cgctggcggc agccctgttc
     1081 gcgatggcga cgaccgggct cgggctggtc gtcctcggcg ggcgccggca ccaggagccg
     1141 gtcgagacgg tggccgttgt gccggcccgg cgcccgcccc gccggatgcc ccgccgccgc
     1201 taaccgtcgc cgggtccgac gtcgaaatgt tcccggcggc actcgtttcg gcgacatccg
     1261 ccgtcgtatc cttccctcag attcgccctc agttggagtg ccctgtggca gcccggggag
     1321 ataccgccgg ccgctgatcc cgattccggg gtctgctttg acgcggccac ctcgctcccg
     1381 cgcccctgcg cgaggagcct gcatcgtcat tccccgcttt ctgctgcctg ctgaaagggc
     1441 accccagtgc aactcgagac gatcttcacc ggccggctgg tccgcctcgc ggcgccgcgc
     1501 cccgacgacc acgagacgtt cgctcgctgg agcgagaacg accagtacct tcgcacgatg
     1561 gacaatgacc cggcccgccc gatctcggcg gaggcccacg ccgcctggga ggcgccgttc
     1621 ctccaatccc cgaccagctt catcttccgg atccgcaccc tggcggacga cgccctgatc
     1681 ggcgtcgtcg ccctcggcga cgtccagtgg atgcaccagt gcgccatgct cggcatcgcc
     1741 atcggcgacc ccgcctgctg gggcaagggg tatggcagcg acgccatcga cctgatgctg
     1801 cgctacgcct tcctggagct gaatctgtac cgcgtctggc tcaccaccat cgcttacaac
     1861 gagcgcgccc ggcgggcgtt cgagaaggcc ggcttccggc cggagggcgc ctggcgggag
     1921 atgatccacc gcgatggcaa gcggttcgac gtgctccact acgggctgct gcgccacgag
     1981 tgggaagccc atcgaaccta atctcgtcct tccctcccct cccccgcgca cgggggaggg
     2041 gaggggggta ggggcaactt gggggtaggg gaaacttccg ctggccgccc ggccggccgg
     2101 tccgctatcg tgatcgccgt gacgcagcac cagggtcccg tcgacctctc agaccttcgc
     2161 cagcggatgg acgatctcga ccagcagctc gtcgagttgc tcgcacaacg ggccgagatc
     2221 tcccgcgagg tcgggcggcg caagggcacg cagggcggcg ccgtcttcgc gccggctcgc
     2281 gagtccgaga tct
//
LOCUS       DASRIQ010000134         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_578460, whole genome shotgun sequence.
ACCESSION   DASRIQ010000134 DASRIQ010000000
VERSION     DASRIQ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578460"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFD06_03170"
     CDS             complement(<1..430)
                     /locus_tag="VFD06_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZN02568.1"
                     /translation="MRAEFGPFTLDSDTRQLRRGQADLHLSPKAFDLLCLLLEHRPKV
                     LEKADLHARMWPDTHVVDGSLNVLITEIRRALGDDARDPTFIRTAHGVGYAFCGEATA
                     AAPAARRAAMRCWLAEAGRTIRLNEGENVVGRDPECSVWLD"
     gene            693..1511
                     /locus_tag="VFD06_03175"
     CDS             693..1511
                     /locus_tag="VFD06_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN02569.1"
                     /translation="MIDAVVENMRQNLEQLKYSTLAPSRYTVYLHPNEYARLEGIIPV
                     LRQQTVQALSEELEKLNGRPSLKGLIQRWRGGAAEPHYQSAAGDWQIEFLADPDGEMT
                     EGDLLIDSELILPAIPDLGVGERTRRITTTHSGQRTATREQTINPTPPAAVKAVFARI
                     EYDDDGGHHLYDVAKDAVSIGRGGIAFPVDVRIVSSPDVSREHARLRRDPQTGRFFLI
                     DLSTLGTTLNGRHVPKGYDEVDGTRRENGAETALPDTATIGLADTVVLEFRIVR"
     gene            1532..>2552
                     /locus_tag="VFD06_03180"
     CDS             1532..>2552
                     /locus_tag="VFD06_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZN02570.1"
                     /translation="MSAVRAAGNTDPGLQRDVNEDRFHLDLARGLFMVVDGVGGQAAG
                     GKAADTAISLLRTRLEREAGPATMVDRLREAITVANNEIHRLAGLRQEWAGMACVLTI
                     AVVDEGRAVIGHVGDTRLYKLHRGRMEKVTRDHSPVGEREDAGELSELEAMNHPRRNE
                     VYRDVGSESHAARDPEFIEVHDVPFEADAALLLCSDGLTDVVDSSSIQRVVAQWAGHP
                     QQVVDALIEAANAAGGKDNVTVVYVEGEQFALASAGLGAPDQEITRRLVPAGTRQEAP
                     PPRSTAAADPERSRGRRERVVRSILVFLLTVVILLVVFQSVPELPLVGPQAPGNSAAD
                     TWRVTV"
BASE COUNT          404 a          883 c          875 g          390 t
ORIGIN      
        1 agtccagcca gacgctgcat tcgggatcgc ggccgaccac gttctctccc tcgttgaggc
       61 ggatcgtcct gcccgcctcg gccagccagc agcgcatcgc cgcgcgccgc gcggccgggg
      121 cggccgccgt cgcctccccg cagaacgcgt agccgacgcc atgcgcggtc cggatgaacg
      181 tcgggtcccg ggcgtcgtcg ccgagggcgc gccggatttc ggtgatcagc acgttgaggc
      241 tgccgtcgac tacatgggtg tcgggccaca tccgggcgtg gaggtccgcc ttctcgagca
      301 ccttcggacg gtgctccagc agcaggcaga ggagatcgaa ggccttggga gacaggtgca
      361 ggtccgcctg cccgcgccgc agctgccgcg tgtccgaatc gagggtgaat ggcccgaact
      421 ccgctctcac gctaatagtt tctttcgaat ttccttaaga cacaggatgc cccggttgcg
      481 ggatagttgt gatcggcgga tcgggcggag ggggacagtc acgtttgtga cctccccgga
      541 gggggactgt ccccctcccc gcgggtcaca aacgtgactg tccccctccg cccccgaaac
      601 ggaacccggc gatgtcccat cccgtcatga ttggaatgcc tgcgccccgc accggcccgg
      661 ccgggacgcc gccccggcgg cccgcccggg atgtgatcga cgcggtcgtc gagaacatgc
      721 ggcagaacct cgagcagctc aaatactcga cgctcgcgcc cagccgttac acggtctacc
      781 tgcatccgaa cgagtacgcc cggctggaag ggattatccc cgttctccgg cagcagaccg
      841 tgcaggcgct ctccgaggag ctggagaagc tcaatgggcg gccgtcgctc aagggcctca
      901 ttcagcgctg gcggggcggc gccgccgaac cgcattatca gagcgcggcc ggggactggc
      961 agatcgagtt cctcgccgac ccggatggcg agatgacgga aggcgacctc ctgatcgact
     1021 ccgagctcat cctcccggcc attccggatc tgggcgtcgg cgagcgcacc cgccgcatca
     1081 ccacgacaca ctccggccag cggaccgcga cgcgcgagca gaccatcaac ccgacgccgc
     1141 cggccgcggt gaaggcggtg ttcgcgcgca tcgagtacga cgacgacggg ggacatcatt
     1201 tgtacgacgt ggcgaaggac gcggtcagca tcgggcgggg tggcatcgcc tttcccgtcg
     1261 acgtccgcat cgtgtcgtcg cccgacgtgt cgcgcgagca cgcccggctc cgccgcgacc
     1321 cgcagactgg gcgcttcttc ctgattgatc tgagcaccct ggggaccacc ctcaacggac
     1381 gtcacgtccc gaaagggtac gacgaggtgg acggcaccag gcgcgagaac ggcgcggaga
     1441 cggcgctgcc cgacaccgcc acgatcggcc tggccgacac cgtcgtgctc gagttccgta
     1501 tcgtccgatg acacccagcg gatccctgaa cgtgagcgcc gtgcgcgcgg ccggcaatac
     1561 cgatcctggc ttgcagcgcg acgtcaacga ggatcgtttc catctcgatc tggcgcgcgg
     1621 cctcttcatg gtcgtcgacg gcgtcggcgg ccaggcggcc ggcggcaagg cggcggatac
     1681 cgccatcagc ctcctccgga cgcggctcga acgcgaggcc gggccggcga cgatggtgga
     1741 ccggttgcgc gaagcgatca cggttgccaa caacgagatc catcgccttg ccggcctgcg
     1801 ccaggagtgg gcggggatgg cgtgcgttct cacgatcgcg gtcgtggacg aggggcgtgc
     1861 cgtgatcggt cacgttggcg atacccgcct ctacaagctt caccgcggcc gcatggagaa
     1921 ggtgacccgc gatcattcgc cggtcggcga gcgcgaggac gcaggggagc tctccgagct
     1981 cgaggcgatg aaccatccgc gccggaacga ggtctatcgc gacgtcggtt ccgagtcgca
     2041 tgcggcgcgc gacccggagt tcatcgaggt tcacgacgtg ccgttcgagg cggacgcggc
     2101 cctgctgctc tgcagcgacg ggttgaccga cgtcgtcgac tcatcgtcca ttcagcgggt
     2161 cgtcgcgcag tgggccgggc atccgcagca ggtcgtggac gccctgatcg aggccgccaa
     2221 cgccgccggc ggaaaagaca acgtcacggt ggtctacgtg gaaggcgagc agttcgccct
     2281 cgccagcgcc ggcctcgggg cgcccgacca ggagatcacg cggcgcctcg tgccggccgg
     2341 cacgcgtcag gaagcgccgc ctccccgcag caccgctgcc gctgatcctg aaaggagccg
     2401 cggacgccgg gagcgcgtcg ttcgatcgat cctggttttc ctgctgaccg tggtgatcct
     2461 gctggtggtc tttcaatccg ttcccgagct gcctctggtc ggcccccagg cgcccgggaa
     2521 tagcgcggcc gatacctggc gggtgacggt gc
//
LOCUS       DASRIQ010000135         4242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_826267, whole genome shotgun sequence.
ACCESSION   DASRIQ010000135 DASRIQ010000000
VERSION     DASRIQ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826267"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /gene="thiC"
                     /locus_tag="VFD06_03185"
     CDS             <1..892
                     /gene="thiC"
                     /locus_tag="VFD06_03185"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZN02571.1"
                     /translation="TVHCGVTREAVRRLDGSGRVAGIVSRGGSMLASWIARSGEENPL
                     FAQFDRLLDIAFEHDVTLSLGDGIRPGATADATDAAQIQELLTLGELTARAHARGVQV
                     MIEGPGHVPIDQIEANVLLEKRVCRGAPFYVLGPLTSDVGAGYDHITGAIGGAVASAA
                     GADVLCYLTPAEHLRLPTIEDVRDGVIATKIAAHSGDMAKGVFWARERDAQMSVYRKK
                     LDWEGQYRLALDPEKAREFRYLSEDYDKAVCTMCGTLCSMALENAREKSGTVLNTSPE
                     QARHRLVPVNIPVVPREIRA"
     gene            889..2100
                     /gene="mqnE"
                     /locus_tag="VFD06_03190"
     CDS             889..2100
                     /gene="mqnE"
                     /locus_tag="VFD06_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HZN02572.1"
                     /translation="MTAIRLSRLEHRALEAAGLSTIRDKVVAGERLSREDGLALYHGA
                     DLLAIGALANRVREARHGDAAYFIVNQHINHTNICVAGCKFCAFYRTAKQKDAYTLSP
                     EDVERRMRQHLDEPIREIHMVGGVNPALPLQYYVDVIRAMKRVRPEVHVKAFTMVEIA
                     AMAENFEMPVEEVLLRLKEAGLGSLPGGGAEILTDHAHQVLFKGKIDHRAWIEVAKTA
                     HRLGLKSNATMLYGHVETVEERIDHMLALREAQDETMGFMAFIPLAFHPENTRLSHLH
                     KPSAADDLRQIAVGRLMLDNFPHIKAYWIMMTPRVAQAGLHFGADDLDGTVVTEEIFH
                     RAGATSPQLVARQRLVDLIEEAGRVPVERDTLYRPVAAAASREDAGAATQAVPPPAFA
                     APTAPAAGAAH"
     gene            2131..2997
                     /locus_tag="VFD06_03195"
     CDS             2131..2997
                     /locus_tag="VFD06_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZN02573.1"
                     /translation="MLVHLNGRLVEEAEAVLPVFDRGFLYGDGVFESMRATHGRIFRL
                     EAHLERMERSAALAEFEGLPHRSTLAAELADLLRANRLAEARLRLTLTRGAGRPGDYV
                     GSQGAPTRVATAVPFQGLDPLHYERGVALRVSRRQAVPGAVLDAAIKTTSRLAAVLAR
                     REAARAGAFEAALLDASGNLTEGTTSNLFLVREGRVLTPAIDGNALPGVTRAVVLETA
                     RSSGIPVDECPLPAALLNVADEIFLTNTSWEVLPVTSVDGRPAGGGRPGPITATLLSG
                     YRAQVRRECGPS"
     gene            3006..>4242
                     /locus_tag="VFD06_03200"
     CDS             3006..>4242
                     /locus_tag="VFD06_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HZN02574.1"
                     /translation="MPPGPLAGSGAPRVTTLDRWIDPLAALRAVRDRRKPVLLYSGLA
                     GSHPEARHSILAWDPLATITVADGEVRLAPDGDEASCPPWTARAADPFPVLRALLPAD
                     GASARGLPFAGGALGYLGYGLRAASERLERAAPEPLGLPDAWFGVYDAALVFDHRSRV
                     VHAIDLPRPGAPSAAGRRLGLERLAAAGDAPAPPTGALAAAAGGLDAARARTGTPRAG
                     YEAAVRDVLERIARGDLYQVNLSHRVEAPLRSGPHDLFERLMRDNPAPFSAYLDAGPF
                     QLLSASPERFVALRGDRAQSSPIKGTRPRGAFPEEDLLHADDLRQSAKDRAENVMIAD
                     LVRNDLGRVCLPGSVAVESLCRLESFATVHHLVTTVAGRLRPDQDRFDLLRALFPGGS
                     MTGAPKVAAMQAIAALEREE"
BASE COUNT          568 a         1575 c         1527 g          572 t
ORIGIN      
        1 cacggtgcac tgcggcgtca cccgcgaggc ggtccgccgc ctcgatggct ccggccgcgt
       61 cgccgggatc gtgtcgcggg gcggctcgat gctcgcctcg tggatcgccc gcagcggcga
      121 ggagaacccg ctcttcgcgc agttcgatcg cctcctcgac attgccttcg agcacgacgt
      181 caccctgtcg ctcggcgacg gcatccgtcc cggggccacc gccgacgcca ccgacgcggc
      241 gcagatccag gaactcctga ccctcggcga gctgaccgcc cgggcgcacg ctcgcggggt
      301 gcaggtcatg atcgaagggc cggggcacgt gccgatcgac cagatcgagg cgaacgtcct
      361 gctcgagaag cgggtctgcc ggggcgcgcc gttctacgtc ctcgggccgc tgacgagcga
      421 cgtcggagcc gggtacgacc acatcaccgg cgccatcggg ggagccgtgg cctcggccgc
      481 cggggccgac gtgctgtgct acctgacgcc ggcggagcac ctgaggctgc cgaccatcga
      541 ggacgtccgc gacggcgtca tcgccacgaa gatcgccgcc cactccggcg acatggcgaa
      601 gggggtcttc tgggcgcgcg agcgggacgc gcagatgtcg gtctaccgca agaagctgga
      661 ctgggagggg cagtaccgcc tcgccctcga ccctgagaaa gcgcgcgagt tccgctacct
      721 gagcgaggac tacgacaagg cggtctgcac catgtgcggg acgctctgct cgatggcgct
      781 ggagaacgcc cgggagaagt ccggaacggt tctgaacacg agcccggagc aggcgcgcca
      841 tcgcctcgtg ccggtcaaca tcccggtggt cccgcgcgag atccgggcgt gacggcgatc
      901 cggctgtcgc gcctggagca ccgcgcgctc gaggcggccg ggctctcgac gatccgggac
      961 aaggtcgtcg cgggggagcg gctgtcgcgc gaggacggcc tcgcgcttta ccacggggcc
     1021 gatctcctgg ccatcggggc cctcgccaac cgggtgcgcg aggcgcgcca cggggacgcc
     1081 gcctacttca tcgtcaacca gcacatcaac cacacgaaca tctgcgtcgc cggctgcaag
     1141 ttctgcgcgt tctaccgcac cgccaagcag aaggacgcct acacgctcag ccccgaggac
     1201 gtggagcggc gcatgcggca gcacctcgac gaaccgatcc gggagatcca catggtcggc
     1261 ggggtcaacc cggcgctgcc gctgcaatac tacgtggacg tcatccgggc catgaagcgg
     1321 gtccgccccg aggtgcacgt caaggcgttc accatggtcg agatcgccgc catggccgag
     1381 aatttcgaga tgccggtgga ggaggtcctc ctgcgcctga aggaggccgg gctcggctcg
     1441 ctccccggcg gcggcgcgga gatcctgacc gaccacgccc accaggtgct gttcaagggg
     1501 aagatcgatc accgcgcctg gatcgaggtc gcgaagaccg cccaccgcct cggcctgaag
     1561 agcaacgcga cgatgctgta cgggcacgtc gagacggtcg aggagcggat cgatcacatg
     1621 ctcgccctgc gcgaggcgca ggacgagacc atgggcttca tggcgttcat cccgctcgcc
     1681 ttccacccgg agaacacccg gctgtcgcat ctccacaagc cttcggcggc ggacgatctg
     1741 cggcagatcg ccgtcgggcg cctgatgctc gacaacttcc cgcacatcaa ggcgtactgg
     1801 atcatgatga cgccgcgcgt cgcccaggcc ggtctgcact tcggggcgga cgacctcgac
     1861 ggcaccgtcg tcaccgagga gatcttccac cgcgccggcg ccacctcgcc ccagctcgtg
     1921 gcgcgccagc gtctcgtcga tctcatcgag gaggcggggc gcgtgccggt ggagcgggac
     1981 acgctctacc gcccggtggc cgccgccgca tcccgggagg acgcgggcgc tgcgacgcag
     2041 gcggtcccgc caccggcgtt cgccgccccg acggctcccg ccgccggcgc ggcgcactga
     2101 gccggtcggt tcgcggggcg ggtccctcag gtgctcgtcc acctcaacgg acgcctcgtc
     2161 gaggaggccg aggcggtcct cccggtcttc gaccgcggct tcctctacgg cgacggcgtg
     2221 ttcgaatcga tgcgcgccac ccacggtcgc atcttccgcc tcgaggcgca cctcgagcgg
     2281 atggagcgct ccgccgccct ggccgagttc gaaggactgc cacaccgttc cacgctcgcg
     2341 gctgagctcg ccgacctgct gcgggcgaac cggctcgcgg aggcgcgcct gcgtctcacg
     2401 ctcacacgcg gggcgggccg gccgggggac tacgtcggct cccagggagc gccgacgcgc
     2461 gtggccaccg cggtgccgtt ccaggggctc gacccgctcc actacgaacg gggcgtggcg
     2521 ctccgggtgt cccgccgtca ggcggtcccc ggggcggtgc tcgacgcggc catcaagacg
     2581 acgagccggc tcgcggccgt cctggcgcgg cgggaggcgg cccgcgcggg ggcgttcgag
     2641 gcggcgcttc tcgacgcctc ggggaatctg accgaaggga cgacgagcaa cctgttcctc
     2701 gtccgggagg ggcgcgtgct cacgccggcg atcgacggga atgcccttcc cggcgtcacc
     2761 cgcgccgtcg tgctggagac ggcgcgctcc tcggggatcc cggtcgacga gtgccccctc
     2821 cccgccgcgc tcctgaacgt ggcggacgag atcttcctga ccaacacctc gtgggaggtg
     2881 ctgccggtca cgagcgtcga cggccgcccg gcgggaggag gccggccggg gccgatcacc
     2941 gcgacgcttc tctccggata tcgcgcccag gtgcgccgcg aatgcggccc ctcgtagagg
     3001 gcggcgtgcc gccggggccg cttgcgggat cgggagcgcc ccgcgttacg acgctcgacc
     3061 ggtggatcga tcccctggcg gcgctccggg cggtgcggga tcggcgcaag ccggtcctcc
     3121 tctacagcgg tctggcgggc tcgcacccgg aggcgcggca ctcgatcctg gcctgggatc
     3181 cgctcgcgac gatcaccgtc gcggacggag aggtgcggct ggcgccggac ggcgacgagg
     3241 cctcgtgtcc tccatggacc gcgcgcgccg ccgacccgtt cccggtgctg cgcgcgctcc
     3301 tgccggcgga tggggcgtcc gcccgggggc ttccgttcgc gggcggcgcc ctcggctatc
     3361 tcggatacgg gttgcgcgcc gcttcggagc ggctcgagcg cgccgccccc gagccgctcg
     3421 gtctgccgga cgcctggttc ggcgtctacg atgctgccct cgtcttcgat caccggagcc
     3481 gcgtggtgca cgcgatcgac ctgccgcggc ccggcgcgcc gtccgcggcg gggcggcgcc
     3541 tgggcctgga gcgcctcgcc gccgccggag acgcgccggc tccccccacc ggtgcgctcg
     3601 ccgccgcggc gggcggactc gacgccgccc gggcgcgcac cgggacgccg cgggccgggt
     3661 acgaggcggc ggtccgggac gtgctcgagc gcatcgcgcg cggcgacctg taccaggtga
     3721 acctgtcgca tcgcgtcgag gcgccgctcc gatcgggccc ccacgacctg ttcgaacggc
     3781 tgatgcgcga caacccggcc ccgttttccg cgtacctcga cgccggcccg ttccagctcc
     3841 tgagcgcgtc gccggagcgg ttcgtcgcgc tccgcggcga ccgggcgcaa agcagcccca
     3901 tcaaggggac ccgcccgcgc ggcgccttcc ccgaggagga cctcctccac gccgacgacc
     3961 tgcgccagag cgccaaggac cgggcggaga acgtcatgat cgcggacctg gtgcggaacg
     4021 acctcgggcg ggtctgcctc cccggcagcg tcgccgtcga gtcgctgtgc cgcctcgagt
     4081 cgttcgccac ggtgcaccat ctcgtcacca ccgtcgcggg gcggctgcgg cccgatcagg
     4141 atcgcttcga tctcctgcgc gccctcttcc cggggggctc gatgaccggc gcgccgaagg
     4201 tggccgccat gcaggcgatc gccgcgctcg agcgggagga gc
//
LOCUS       DASRIQ010000136         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_578740, whole genome shotgun sequence.
ACCESSION   DASRIQ010000136 DASRIQ010000000
VERSION     DASRIQ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578740"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFD06_03205"
     CDS             complement(<1..462)
                     /locus_tag="VFD06_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZN02575.1"
                     /translation="MPAATKRKPKAPKRAARPVPAGLEPPPDRPLVNPGLSRAQLLEI
                     HRFLRLNRMLEDKLTALYRQGRITGGLYSSLGQEAISVGTAYALAPNDLLAPMIRNIG
                     SMLVRGVTPLDFFQQYMGRYESPTHGKDGTLHFGNVEKLSLIGPISHLGTLV"
     gene            complement(467..1840)
                     /gene="lpdA"
                     /locus_tag="VFD06_03210"
     CDS             complement(467..1840)
                     /gene="lpdA"
                     /locus_tag="VFD06_03210"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZN02576.1"
                     /translation="MIGSGPGGYVAAIRAAQLGLKTAIVEKYAALGGTCLHVGCIPTK
                     ALLHTADVLETAKDAARFGVTTGNVGLDLAAMHKYRTDVIRRQAKGVEYLMKKNNITV
                     LAGHGRLKGVGKVEVKPESGAAQTIATKHVILATGSAAKMLPGLKADAKRILTSTEAL
                     LLERVPKSMIVLGAGAVGVEFASIYARFGAQVTIVEMLPRVLPIEDEEISAEMQKALK
                     KRGIEVRVATKVEEVKPAEKGVEVKVTSEKGSEVLKGETLLVAVGRRPVTEDLGLDTT
                     KVQVDKGFVKVDARMRSTEPTIYAIGDIVPTPMLAHLASHEGIVAAEAIAGKNPRPIN
                     YDHVPNATYSEPEVASVGLTEATAKSRGHKVKVGKFPIPPLGKGRILNAQEGFVKLVG
                     DERYDELLGVHIIGPKATELIAEGGTALQLESTVEALHEAIHAHPTLSEAMGEAALAL
                     HARGIHL"
     gene            complement(1996..3135)
                     /locus_tag="VFD06_03215"
     CDS             complement(1996..3135)
                     /locus_tag="VFD06_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN02577.1"
                     /translation="MNDWKRHFGDFGGRAYLDCAAQGPFPRDVSEEIQRQLRLKEHPE
                     EIGPDLFERLPERAAVAAARLLGCHPSSMTFGSGASHGLNAAALGLPLRDGDEVLVGR
                     GEFPAVVLPFVNLAPAGVRVRFVHPGAGGRHVAADDFIAAFSPKTRAVAVSLVAFATG
                     YRIDLDRLGEACRERGIFLVVDGSQGIGGIEFRVADHPVDVLAVSGYKWLFGPYGTGL
                     TYISPRVLDRLRVAVVNWQTVEGAAQLNRPFDYALRFREGACRFDAPQRASFLNLGGF
                     AASMEFLARVRVSTIEAHATRLLDRLLLGLGSTVLKCASDLAPKHRSTILGLEAESFD
                     ATRRAWRRLVEAGVVVSLRANLIRVAPNIYNEPEDIDRLLKAAAR"
     gene            3226..>3409
                     /locus_tag="VFD06_03220"
     CDS             3226..>3409
                     /locus_tag="VFD06_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02578.1"
                     /translation="MTNLPSTERAARFLSPPPEAVITNAFVRPFDNVVATARTCYSSG
                     GIVRDDQILGGPEVPEE"
BASE COUNT          481 a         1204 c         1176 g          548 t
ORIGIN      
        1 cacgagcgtc ccgaggtggc tgatcggccc gatgagggac agcttctcga cgttgccgaa
       61 gtggagcgtc ccgtccttcc cgtgggtggg ggactcgtac cggcccatgt actgctggaa
      121 gaagtccagc ggcgtcacgc cgcgcaccag catcgatccg atgttgcgga tcatcggggc
      181 cagcaggtcg ttcggcgcca gcgcgtacgc cgtgccgacc gagatcgcct cctgccccag
      241 gctgctgtag aggcctccgg tgatgcgccc ctgccggtag agcgcggtga gcttgtcctc
      301 gagcatccgg ttgagccgca ggaagcggtg gatctcgagg agctgcgccc ggctcagacc
      361 gggattgacg agcggccggt ccggcggcgg ctcgagcccg gccggaacgg gacgcgcggc
      421 gcgcttcggt gctttgggct tgcgcttcgt cgcggcgggc acttgcctag aggtggatcc
      481 cacgggcgtg gagcgccagg gccgcctccc ccatcgcctc gctcagggtc gggtgggcgt
      541 ggatcgcctc gtgcagcgcc tcgaccgtcg actcgagctg gagcgccgtc cctccctcgg
      601 cgatcagctc ggtcgccttc ggcccgatga tgtgcacccc gagcagctcg tcgtaacgct
      661 cgtctcccac gagcttcacg aacccttcct gggcattgag gatgcgcccc ttgccgagcg
      721 gcgggatcgg gaatttcccc actttgacct tgtggccgcg cgacttcgcc gtcgcctcgg
      781 tcaggccgac gctcgccacc tccggctcgc tgtaggtggc gttggggacg tggtcgtagt
      841 tgatggggcg cgggttcttg ccggcgatcg cctccgccgc gacgatgcct tcatgggagg
      901 cgagatgcgc cagcatcggt gtcggcacga tgtcgccgat ggcgtagatg gtcggctcgg
      961 tgctgcgcat ccgcgcgtcc accttcacga accccttgtc gacctgcacc ttggtcgtgt
     1021 cgagcccgag gtcctccgtg accgggcgcc ggccgacggc gacgaggagc gtctcccctt
     1081 tcaggacctc gctccccttc tccgacgtca ccttgacctc gacccctttc tcggccggct
     1141 tgacctcctc gaccttggtc gcaacgcgca cctcgatgcc gcgcttcttg agcgccttct
     1201 gcatctcggc ggagatctcc tcgtcctcaa tcggcaggac gcgcggcagc atctcgacga
     1261 tcgtcacctg cgcgccgaag cgggcgtaga tcgacgcgaa ctcgacgccg accgcccccg
     1321 cgccgagcac gatcatcgac ttcggcaccc gctcgagcag gagcgcctcg gtgctggtca
     1381 ggatgcgctt ggcatccgcc ttgaggccgg ggagcatctt ggccgccgat ccggtcgcaa
     1441 ggatgacgtg cttcgtcgcg atggtctgcg ccgctccgct ctccggcttc acctcgacct
     1501 tgcccacccc cttgaggcgg ccgtggccgg cgagcaccgt gatgttgttc ttcttcatca
     1561 ggtactcgac ccccttggcc tgccggcgga tgacgtcggt gcggtacttg tgcatggcgg
     1621 cgaggtcgag cccgacgttc ccggtggtga cgccgaagcg ggccgcgtcc ttcgccgtct
     1681 ccaggacgtc ggcggtgtgc aggagcgcct tcgtcgggat gcacccgacg tgcaggcagg
     1741 tgccgccgag cgccgcatac ttctcgacga tcgccgtctt caacccgagc tgcgccgcgc
     1801 ggatcgcggc gacgtagccg ccgggaccgg aaccgatcac cgtcacatcg aaagattggg
     1861 gttcgggcac gcatgcctcc gatgaaccat ggcgggaatg ggcgcggatt ctagcacgga
     1921 gccatgatcc ggcgggcgat ccgcacctcc ggagggttgc gcctccaggc gtcccggccg
     1981 gatcgtgccg tcgggctatc gtgcggcggc tttgaggagc cggtcgatgt cttccggttc
     2041 gttgtagatg ttcggggcga cccggatgag gttcgcgcgc aggctgacca cgacccccgc
     2101 ttccacgagc cggcgccagg cgcgacgggt ggcatcgaag ctctccgcct cgaggcccag
     2161 gatcgtcgag cggtgtttcg gggccaggtc ggaggcgcac ttcaggacgg tcgagccgag
     2221 cccgaggagc agccggtcga ggaggcgcgt cgcgtgcgcc tcgatggtcg agacgcgcac
     2281 acgggccagg aattccatcg acgccgcgaa tcccccgagg ttgaggaacg aggcccgctg
     2341 cggcgcgtcg aagcggcacg ccccctcgcg gaagcgcagc gcgtagtcga acgggcggtt
     2401 cagctgcgcc gccccctcca ccgtctgcca gttcaccacc gccactcgca ggcgatcgag
     2461 gacgcgcggg ctgatgtagg tgagcccggt cccgtacgga ccgaagagcc acttgtagcc
     2521 gctgaccgcc aggacgtcga ccgggtggtc ggcgacgcgg aactcgatcc cgccgatccc
     2581 ctgcgagccg tccacgacga gaaagatgcc gcgctcgcgg cacgcctcac ccagccggtc
     2641 gaggtcgatg cggtagccgg tcgcgaaggc caccaggctc acggcgacgg cgcgagtctt
     2701 cggcgagaag gcggcgatga agtcgtcggc ggcgacgtgc cggcctcccg cgccggggtg
     2761 gacgaagcgg acccgcacgc cggccggggc gaggttcacg aacggcagga cgaccgccgg
     2821 gaactccccc cgccccacga ggacctcgtc gccgtcccgc agcggcagcc cgagggccgc
     2881 ggcgttgagc ccgtgcgacg cgccggaacc aaatgtcata ctcgaggggt ggcagccgag
     2941 cagccgcgct gcggccactg ccgcccgctc cggcaggcgc tcgaagaggt cgggaccgat
     3001 ctcctccggg tgttccttga ggcggagctg gcgctggatc tcctccgaca catcgcgcgg
     3061 aaagggcccc tgggcggcgc aatccaggta cgcccgcccg ccaaaatccc cgaaatgccg
     3121 cttccagtcg ttcatgcgcg cgtatccgcg gcggatccta cactcgaagg cggccgcacc
     3181 accggtcgcg cgatcgccgg gctataatcc gggcggtcga tgtcgatgac gaatctcccg
     3241 tccaccgagc gcgccgcgcg gttcctgtcg ccgcctcccg aagcggtgat caccaacgcc
     3301 ttcgtgcgcc ccttcgacaa cgtggtggcg acggcgcgca cctgctactc ctccggcggc
     3361 atcgtgcggg acgatcagat cctcggcggc ccggaggtcc cggaggagg
//
LOCUS       DASRIQ010000137         9578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1359077, whole genome shotgun sequence.
ACCESSION   DASRIQ010000137 DASRIQ010000000
VERSION     DASRIQ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359077"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFD06_03225"
     CDS             complement(<1..325)
                     /locus_tag="VFD06_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02579.1"
                     /translation="MSDPTRPPRVDPWLAVTLALWGGYFAWLWPRMLATRADGLYAGW
                     RLLWADWSFHFAYANVFAYSPPSEWLTRHPLFAGIPFAYPFAADAVSGLLMRAGAGRI
                     PAFLIP"
     gene            complement(345..938)
                     /locus_tag="VFD06_03230"
     CDS             complement(345..938)
                     /locus_tag="VFD06_03230"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(669..671),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZN02580.1"
                     /translation="MKRAMPRAAALLTVGLLPFLTAADSPPAPTKAGEPPAAAAAEAV
                     KPLEAGDQAPSFTLQGSDGKSYSLAGFKGKKAVVLAWFPKAFTGGUTAQCKSLRASGS
                     EIRAYDVAYFAASVDDAETNRKFAESLGLDYPILSDPGKVTARAYGVLEGDYANRHTF
                     YIGKDGRILLVDRAVKPSTAGEDTARHLAELKVPRCG"
     gene            1232..1822
                     /locus_tag="VFD06_03235"
     CDS             1232..1822
                     /locus_tag="VFD06_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02581.1"
                     /translation="MTGRNSGGVGPRTGLTGIMAAATILAASLLGLAWANGKSEDRQK
                     PNRPQGTSSTQPAPKAPDTATTSARPVTRTIGTATVSVDPTTGRMQPLSPEETAKLTA
                     SLYKMLSRETEDMLIIQHEDGSRMASLDDAFQEVAMATRDKRGNVRLHCVNQRAQAER
                     LLSGAEDRGPIYNIKATRKGGKKGAAKTAPAPAETE"
     gene            1825..3720
                     /locus_tag="VFD06_03240"
     CDS             1825..3720
                     /locus_tag="VFD06_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HZN02582.1"
                     /translation="MRRVNAAGLLAPIAVFALALSLLPATPAFASATLVIVNDNAPGV
                     GFNDPTPVAPVGGNPGLTLGQQRLNAFQFAADIWGATLDSNVEIEILASFVPLACNAT
                     GATLGSAGTIWIWDNVPANGAFPGSEFVDTWSSQALASKRFGADVNPVPICTNTALPC
                     TTHADCAVPAPAGICSNADIRARFNVNLGNPGCLTGVPWYLGFDNNHGANTIDLVTVL
                     LHEFGHGLGFQQFASLTTGAQPANQTDLYGRRLLDTTTSKTWNQMTNAERVVSAINSR
                     RVVWDNGQVGAEVPGVLAFGVPNMRVNSPGSIAGNFSVGGAQFGPPLNAGGVTGDLVQ
                     ALDAANANGPTTFDACTPMTNAAAVAGNIALVDRGTCGFIVKAANVQAAGAIAMVVAD
                     NAVGSPPPDLGGIDPALVIPAVRITLADGNLIKPLLGSGVNVTLLLDLTIRAGADGMG
                     RAQMNAPNPVVGGSSISHWDPIAFPNQLMEPAINGDLTHNVSGVDLTLALMRDIGWFP
                     DADVEGIADADDNCPDIANADQADNDLDNLGDVCDPDDDNDGVDDDADNCPIDSNVDQ
                     EDFDLDGLGDACDAETGPPQNVDQCKNGGWARFNTPRTFPNQGLCACYVLSTLGERCP
                     SPVTGRN"
     gene            4045..4413
                     /locus_tag="VFD06_03245"
     CDS             4045..4413
                     /locus_tag="VFD06_03245"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN02583.1"
                     /translation="MSSRGCLSLLLPALLTCSFLGCAEDLTTDASVVAPPGAWEDCDD
                     IRDVSLTARLNTLYLVDPYVDASRIAVETHACVVTLRGRVPDPGTRDRAGAMARETDG
                     VRQVRNRLEVVATVADGGRY"
     gene            4726..5397
                     /locus_tag="VFD06_03250"
     CDS             4726..5397
                     /locus_tag="VFD06_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZN02584.1"
                     /translation="MTPIDRDRLLQHYPVLSNIPPDLMKRIEQESVVLQAPAGRRLFG
                     EGSPCTHYPLLVEGVIRATKSSPDGHEILLYRLNPGESCVITVIALLGETAYPAIGSA
                     ESDLLIYGVPRAVFEELILTVPAFRVFVFQYLSQRMAHLMALIDDVAFRRVDQRLASR
                     LLAADAPIAVTHQMLADELGTTREVVSRTLEAFQENGVLKLGRKRIEILDRSALDRAQ
                     RPTAS"
     gene            5536..5706
                     /locus_tag="VFD06_03255"
     CDS             5536..5706
                     /locus_tag="VFD06_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02585.1"
                     /translation="MPKRPLVRLRQDPAGLPLSAPHTLDTCTADHFHSQLTMVTTPTR
                     ALLDDGDGPSSG"
     gene            complement(5801..7162)
                     /locus_tag="VFD06_03260"
     CDS             complement(5801..7162)
                     /locus_tag="VFD06_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZN02586.1"
                     /translation="MNSRWIFPFAVLLGLALWSLGTTEVPQAAGGAPIRAGDVAWLLT
                     ATGLVLLMTPGLSFFYGGMVGARNVISTMLQSFVALGVISLLWVAFGFSLAFGESLYG
                     LIGNPFTYLMFKGVGVGTDAALAPTIPLLLFAMFQLKFAVITPALVSGSFAERVRFSA
                     YLLFMCLFSVFVYAPLAHWTWHPNGFLKQWGVLDFAGGTVVHMSAGFAALAGAIYLGP
                     RLARGRQAGPSQANTPYVLLGTGMLWFGWFGFNAGSALAADAVAAQAFATTNTASASA
                     MLAWMFFDGLRGRKPGALGACIGAVVGLVAITPAAAYVSVPHSILIGVVASLLSNLAV
                     HLKSRSALDDTLDVFPCHGVGGIVGMLLTGILARDVGLLSGEPTIFLRHLMALLIVSG
                     YSFSVSLLLFKLTDLFVSVRVTPEDEAIGLDISQHAERLALVPHPEPAIVTADTDNLR
                     ASG"
     gene            complement(7332..>9578)
                     /locus_tag="VFD06_03265"
     CDS             complement(7332..>9578)
                     /locus_tag="VFD06_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN02587.1"
                     /translation="EADVNFVKPAKTGANLDRATWSVYVGCPTDSDDDGVGDACDTCT
                     DFDRDGFGNAGYPANTCALDNCPAVSNPGQQDTDLDGAGNACDPCTDIDGDGAGDPPL
                     PGNVCAPDNCPSLPNPGQQEGDGDGRGDACDNCPAVANASQTDQDADGRGDACDNCAA
                     LSNPGQQDADADGRGDACDNCPARANPDQADGNADGAGDACQPSLTLTSIREDGGTRL
                     EAQGRAADPEGEALSGSIRFQPIAAVHLLDSGYTGDCGLGWLPDGVTGEGIAYANGSA
                     GGAVLFDLDSNFGCSDGLRDFLIAPGTCAQPQGAFEEAVFFSGEVPPRPFCVRRPGQV
                     GGTDLLVSSYDDGTLDAGANGAIVLTIPWSGPGLPRDSDIAALPASGRFLMTITATDS
                     HTPGLTTERSFLHQGETRLIFNQPPVAVFQAPASVECTSHAGGAVALNGTGSSDPDST
                     PGTANDIALYQWIENPGTPGEIALGTGASLGVTLPRGTHALGLRVTDTVGDASLATGT
                     VVVVDTAAPSLSVTPGATTLWPPNHHLRPVAFGWQAADACDPAPVVTLLSATSGEPDD
                     AAGLDDGDTVGDVAGAQAGTPDAALMLRAERSRAGSGRSYAIAYRALDASGNSVTTPA
                     AVGVPIASGPDPEPLVIRLQPGASPGTARLDWDAVPGATGYDVIAADRSSVGVVDRVL
                     TLGTVRVLATGTTSLFVIETPATPLPPVGGAHLYFVQSRTATGGVGYGTESAPWPRLP
                     TSCPGGCP"
BASE COUNT         1499 a         3451 c         3299 g         1329 t
ORIGIN      
        1 aggggatgag aaacgccggg atgcgccccg ccccggcccg catgaggagt cccgagacgg
       61 catcggcggc gaagggatag gcgaacggaa tgcccgcgaa cagagggtgc cgcgtcagcc
      121 actccgaggg cgggctgtag gcgaagacgt tggcgtaggc gaagtggaac gaccagtcgg
      181 cccagagcag gcgccagccc gcgtacaggc cgtcggcccg ggtcgcgagc atccgcggcc
      241 acaaccacgc gaagtagccg ccccagaggg cgagcgtgac ggcgagccag ggatcgacgc
      301 gcggcggccg ggtcggatcg ctcatccgcc gacggggtgt cgggtcagcc gcagcgcggg
      361 accttgagct ccgccaggtg cctggcggtg tcttccccgg ccgtggatgg cttcaccgcg
      421 cgatcgacga gcaggatgcg gccgtccttg ccgatgtaga aggtgtggcg gttggcgtaa
      481 tccccttcga ggacgccgta ggcgcgggcg gtcacctttc cggggtcgct gaggatcggg
      541 taatcgaggc cgagcgattc ggcgaacttg cggttggtct cggcgtcgtc gacgctcgcc
      601 gcgaagtacg ccacgtcgta cgcgcgaatc tcggagccgc tggcacgcag cgacttgcac
      661 tgcgccgttc acccgccggt gaacgccttg gggaaccagg cgagcacgac cgccttcttc
      721 cccttgaagc cggcgagcga gtaactcttc ccgtcggagc cctggagcgt gaacgacgga
      781 gcctggtccc cggcctcgag cggcttcacc gcctcggccg cggcggcggc gggcggttcc
      841 ccggccttcg tcggggcggg ggggctgtcc gcggccgtca gaaatggaag cagcccgacc
      901 gtcaggagcg ccgcggcgcg cggcatcgct cgtttcatcg gcttccctcc ttcgacagga
      961 agacagtccc cattctggac ccgggttcgc ggccggcgca actacctccc cgcaaccccg
     1021 catccccggc cgattccgaa cggtgtcacc ccttgacacg ccgggccgtt cgcatctata
     1081 ccgtgcgcac gccgtagaaa cgctgagaaa tcgatagtcc ggaaggagaa acccgcgatg
     1141 gcctgaaccg cccccggggc gatcgtcgct ccgcagggca tgcatgcagg tgaagggcag
     1201 ttctctgctc actcgtgaag gagggcagac catgaccggt aggaactcag ggggagtggg
     1261 gccgcgcacc ggcctcaccg gcatcatggc cgcggcgacc atcctcgccg caagtctcct
     1321 cgggctggcc tgggcgaatg gcaagtcgga ggatcgacag aaacccaacc gtccgcaggg
     1381 cacgtcgtcg acccagcccg ccccgaaagc gcccgacacc gccaccacca gcgcccgtcc
     1441 cgtcacccgc accatcggca ccgccaccgt cagcgtcgat ccgaccaccg gccggatgca
     1501 gccgctgtct ccggaggaga cggcgaagct gaccgcgtcc ctctacaaga tgctcagccg
     1561 cgagaccgag gacatgctca tcatccagca cgaggacggc agcaggatgg cgagcctgga
     1621 tgacgcgttc caggaggtcg ccatggcgac cagggacaag agggggaacg tccggctgca
     1681 ctgcgtcaac cagcgcgcgc aggcggagcg gctcctttct ggcgccgagg accgcgggcc
     1741 gatctacaac atcaaggcca cccgtaaggg cggcaagaag ggcgccgcga agactgcccc
     1801 ggctcccgcg gagacggagt aaccatgcgc cgagtcaacg ccgcgggcct gctggccccg
     1861 atcgccgttt ttgccctcgc tctcagcctc ctgccggcga cgccggcctt cgccagcgcc
     1921 acgctcgtca tcgtcaatga caacgccccg ggggtcggct tcaacgaccc gacaccggtc
     1981 gcccccgtgg gtggcaaccc cggtctgacg ctcggacagc agcgcctgaa cgccttccag
     2041 ttcgccgccg acatctgggg ggcgacgctc gacagcaacg tcgagatcga gatcctggcg
     2101 tccttcgtgc cgctcgcgtg caacgcgacg ggggcgacgc tggggagcgc cgggaccatc
     2161 tggatctggg acaacgttcc ggcgaacggg gcctttccgg ggtcggagtt cgtggacacc
     2221 tggtcctcgc aggccctggc cagcaagcgc ttcggggccg acgtcaaccc agttccgatc
     2281 tgcacgaaca cggcccttcc ctgcaccacc cacgccgatt gcgccgtccc ggctccggcc
     2341 ggcatctgct cgaacgccga catccgggcg cgcttcaacg tcaacctcgg caatcccggc
     2401 tgcctcaccg gcgtgccgtg gtacctgggc ttcgacaaca accacggcgc gaacaccatc
     2461 gacctggtga cggtgctcct gcacgagttc ggccacggcc tcgggttcca gcagttcgcg
     2521 agcctgacga ccggcgcgca gcccgccaac cagaccgacc tctacggccg caggctcctc
     2581 gacaccacga ccagcaagac ctggaaccag atgaccaacg ccgagcgcgt cgtctccgcg
     2641 atcaactcgc ggcgcgtggt gtgggacaac ggccaggtcg gcgcggaggt ccccggggtc
     2701 ctggcgttcg gcgtgccgaa catgcgggtc aactcccccg gctcgatcgc cggcaacttc
     2761 tcggtcggcg gggcccagtt cggtccgccg ctcaacgccg gcggcgtgac cggcgacctc
     2821 gtccaggcgc tggacgcggc caacgcaaac gggcccacca ccttcgacgc ctgcaccccc
     2881 atgaccaacg ccgcggcggt cgccggcaac atcgcgctcg tggaccgcgg cacctgcggg
     2941 ttcatcgtca aggcggcgaa cgtgcaggcg gccggggcga tcgcgatggt ggtggccgac
     3001 aacgccgtcg gctcgccacc gcccgatctc ggcggcatcg atccggcgct cgtcatccct
     3061 gcggtgcgca tcaccctggc ggacgggaac ctcatcaagc cgctccttgg ctcgggagtg
     3121 aacgtcaccc tgctcctcga cctgacgatc cgcgccggcg cggacggcat gggccgggcg
     3181 cagatgaacg cgccgaaccc ggtcgtcggc ggctcgtcga tctcgcactg ggatccgatc
     3241 gccttcccga accagctgat ggagccggcg atcaacggcg acctgacgca caacgtcagc
     3301 ggcgtcgacc tgacgctcgc cctgatgcgc gacatcggct ggttcccgga cgccgacgtc
     3361 gaggggatcg ccgacgccga cgacaactgc ccggacatcg ccaacgccga ccaggccgac
     3421 aacgatctgg acaacctcgg cgacgtctgc gacccggacg acgacaacga cggcgtcgac
     3481 gacgacgccg acaactgccc gatcgactcc aatgtcgatc aggaagactt cgacctcgac
     3541 ggtctcggcg acgcctgcga tgcggagacc ggtccgccgc agaacgtgga tcagtgcaag
     3601 aacggcggct gggcgcggtt caacacgccg cgcaccttcc cgaaccaggg gctctgcgcc
     3661 tgttacgtgc tctcgacgct cggcgagagg tgcccgagcc cggtgaccgg aagaaactag
     3721 accttccggc ctgaaagaac gggccgccgc gctccggtgt ccgcaccgga gcgcggcggc
     3781 cgactccggg tccggctccg gaggacgctc cccatcctcg ccagaggaca cgccgctcgg
     3841 taattcccgc aatgcggccg ggagaatttc ccgacagcaa ccttccgtat tcccgcgttt
     3901 acgggcctcc gcgcgcttgg tgtccgcggg caccgatctt gcttttttcc cagcagcgtc
     3961 gccacagcaa gacagcgcgt cgccacggca agacagaaaa gtgcacgccg ccccccccgc
     4021 ggcgttgtga aggaggagga cgtcatgtct tcgcgcggct gtctgtccct gctcctgccg
     4081 gccctgctca cctgcagctt cctcggctgc gccgaggacc tcacgacgga cgcgtcggtc
     4141 gtggcccctc ccggagcgtg ggaggactgc gacgacatcc gcgacgtctc cctgaccgcg
     4201 cggctcaata ccctctacct cgtcgatccg tacgtggacg cctcccgtat cgcggtcgag
     4261 acgcacgcct gcgtcgtgac gctgcgcggg cgcgttccgg accccggcac gcgcgatcgc
     4321 gccggagcga tggcgcgcga gaccgacggg gtgcgccagg tgcgcaaccg gctcgaggtc
     4381 gtcgcgacgg tcgcagacgg ggggcggtat tgagcatgtc gttctgccgc ggactcgtcc
     4441 cggcgcacaa gtcaccgagg ccccggcatt cgggccgggc cattcacata ctggttgcgg
     4501 cgggggccgc gctgtgggtc accgtggaac cggcccccgc cgacatccag accttcgccg
     4561 gcatcgcgcc gggcgcccac gacggtccgc tcgaccgggg tcgctgagac ccgtctcccg
     4621 cgagcgcacc caagccccgc cccccgtcgc tgcagttcgg accttcccgg ccacccgcat
     4681 ggcaggtatc atggtgacgc tcaacaccat tgtgaggacg ccgccatgac tccgatcgac
     4741 cgtgaccggt tgctgcagca ttaccccgtc ctgtccaaca ttccccccga cctcatgaag
     4801 cggatcgagc aggagtcggt ggtgctccag gcccccgccg gccgccggct cttcggcgaa
     4861 gggagcccct gcacgcacta tccgctcctg gtcgaggggg tcatccgcgc gacgaagtcg
     4921 agccccgacg gccacgagat cctgctgtac cggctgaacc cgggtgagag ctgcgtcatc
     4981 acggtgatcg cgctcctggg ggaaaccgcc tatccggcga tcggttcggc cgaatccgat
     5041 ctgctgatct acggcgtgcc tcgcgcggtc ttcgaggagc tgatcctgac ggtgccggcg
     5101 ttccgcgtct tcgtgttcca gtacctgtcg cagcgtatgg cccacctgat ggcgctcatc
     5161 gacgatgtcg ccttccggcg cgtcgaccag cgcctggcgt cgcgcctgct cgccgccgac
     5221 gccccgatcg ccgtgacgca ccagatgctc gcggacgagc tcggcacgac ccgcgaggtg
     5281 gtcagccgca cgctcgaggc gttccaggag aacggagtgc tgaagctcgg tcgcaagcgc
     5341 atcgaaatcc tcgaccgaag cgccctcgat cgggcgcagc gacctaccgc cagctagaaa
     5401 gacggaactg caaaggccgt agccctcacg tttgggaggc gcggaatgcg gcaggcccgc
     5461 ggggcacgcg gcgcgctatg cccggggccc taggtcacgc gccgctctcg ccgcaaaacc
     5521 gccgcccgtc tgctcatgcc gaaacggccg ctcgtgcggc tgcggcagga ccccgcgggc
     5581 ctgccgcttt ccgcgccgca caccctcgat acctgcaccg ccgatcactt ccactctcaa
     5641 ctcaccatgg tcaccacgcc cactcgtgcg cttctcgacg atggggacgg cccgtcctcc
     5701 gggtgatagg ggtgggacgt tgactcccct ccaccaccct tgccggagga cgggcaatcc
     5761 ctctggtcgg caggggggac tcgactggtt gggggtgacg ttacccgctg gcgcgcaggt
     5821 tgtcggtatc cgcggtcaca attgcgggtt caggatgcgg aacgagggcg agccgctcgg
     5881 cgtgctggct gatgtccaga ccgatcgcct cgtcctccgg cgtgacccgc accgacacga
     5941 acagatcggt gagcttgaac aggagcagcg acacgctgaa ggagtagccg ctgacgatga
     6001 gcagggccat caggtggcgc aggaagatcg tcggctcccc ggagaggagc cccacgtcgc
     6061 gcgccaggat gccggtgagg agcatcccga cgatgccgcc gacgccgtga cacgggaaga
     6121 catcgagcgt gtcgtcgagc gcggagcggc tcttgaggtg caccgccagg ttgctcaaga
     6181 ggctcgccac gaccccgatg aggatgctgt gcggcacgct gacgtacgcc gcggccggcg
     6241 tgatcgcgac gaggcccacg acggcgccga tgcaggcgcc gagcgccccc ggcttgcggc
     6301 cgcgcagacc gtcgaagaac atccaggcga gcatcgccga ggccgacgcg gtgttggtcg
     6361 tcgcgaacgc ctgcgccgcc acggcgtcgg ccgcgagcgc cgagccggcg ttgaacccga
     6421 accagccgaa ccacagcatc ccggtgccga ggaggacata cggtgtgttc gcctgcgacg
     6481 ggcccgcctg tcgcccgcgg gccagtcgcg gccccaggta gatggcgccg gcgagcgccg
     6541 cgaaccccgc cgacatgtgg accacggtcc cgccggcgaa gtcgagcaca ccccattgct
     6601 tcaggaagcc gttcgggtgc caggtccagt gggccagcgg cgcgtagacg aagacgctga
     6661 acaggcacat gaacaggagg tacgcggaga agcgcacccg ctccgcgaac gacccgctca
     6721 ccagcgccgg cgtgatgacg gcgaacttca gctggaacat cgcgaacagc agcagcggaa
     6781 tcgtcggcgc cagcgcggcg tcggtgccga cgccgacccc cttgaacatg aggtacgtga
     6841 agggattccc gatgaggccg tagaggctct cgccgaacgc gaggctgaac ccgaacgcga
     6901 cccacagcag gctgatgacg ccgagtgcga cgaagctctg gagcatcgtc gagatgacgt
     6961 tgcgggcgcc gaccatccct ccgtaaaaga aggagagtcc cggggtcatc aggagcacga
     7021 ggccggtcgc ggtgagcagc cacgccacgt ccccggcgcg gatcggggcc cctcccgccg
     7081 cctgcggcac ctcggtggtg ccgagcgacc agagcgccag ccccaggagc accgcgaacg
     7141 ggaagatcca ccgtgagttc atcaggctcc ctttccaccc ctcacgccgg aggtgacgcc
     7201 ccggcaccgt cccgggccga tcggcaggta tgagaactta atgtgccccg cgtggaaatg
     7261 ccagaaaacc gccggtcccg ggtcaggatc cccgccgtac cttcgctcgg gcagccgatc
     7321 ctgccttcga gctaggggca gcccccgggg caggaggtcg gcaggcgcgg ccagggggcc
     7381 gattcggtgc cgtagccgac gcccccggtc gcggtgcgcg actggacgaa gtagaggtgg
     7441 gcgccgccga ccggcgggag cggcgtcgcc ggcgtctcga tgacgaacag ggacgtcgtt
     7501 ccggtcgcca gcacgcgcac cgttccgagc gtcaggacgc gatcgacgac gccgacggag
     7561 gagcggtcgg cggcgatgac gtcgtagccg gtcgcccccg gaacggcgtc ccagtcgagc
     7621 cgcgcggtcc ccggtgacgc gccgggctgc agccggatca cgagcggctc cggatccggc
     7681 cccgaggcga tcggcacacc cacggccgcg ggcgtcgtga cgctgtttcc cgaggcgtcg
     7741 agcgcccggt aagcgatcgc gtacgagcgg ccgcttccgg cgcgtgagcg ctcggcgcgc
     7801 agcatgagcg cggcgtccgg cgtcccggcc tgcgcgccgg cgacgtctcc gaccgtgtcg
     7861 ccgtcgtcga gccccgccgc gtcgtccggc tcgccactgg tggcggacag gagcgtgacg
     7921 accggcgccg gatcgcaggc atccgccgcc tgccagccga atgcgaccgg ccgcaggtgg
     7981 tggttgggag gccagagcgt ggtcgcgccc ggcgtcacgg agagcgacgg cgccgcggtg
     8041 tcgaccacca cgacggtgcc ggtcgccagg ctcgcgtccc ccaccgtgtc ggtgacgcgc
     8101 agcccgagcg cgtgcgtccc gcgcggcagg gtcacgccga gcgaggcgcc ggtgccgagc
     8161 gcgatctccc ccggagttcc cgggttctcg atccattgat agagggcgat gtcgttggcg
     8221 gtcccggggg tcgagtccgg atcgctcgag ccggtcccgt tcagcgcgac cgccccgccg
     8281 gcgtgggacg tgcactcgac cgaggcgggc gcctggaaca ctgcgaccgg gggctggttg
     8341 aagatcaggc gggtctcccc ctggtgcagg aaggaccgct ccgtcgtgag gcccggcgtg
     8401 tggctgtcgg tcgcggtgat cgtcatcagg aaccggccgc tcgcggggag cgcggcgatg
     8461 tcgctgtcgc gcggcagccc cggcccgctc cagggaatgg tcaggacgat cgccccgttc
     8521 gcgccggcgt cgagcgtccc gtcgtcgtac gaggacacca gcaggtcggt cccgcccacc
     8581 tggcccgggc gccggacgca aaacggccgc ggcggaacct ctccggagaa gaagaccgct
     8641 tcctcgaagg ccccctgcgg ctgcgcgcag gtccccgggg cgatcaggaa gtcgcggagg
     8701 ccgtcggagc agccgaagtt gctgtcgaga tcgaacagca ccgcccctcc ggcggagccg
     8761 ttggcgtagg cgatcccttc gccggtgacg ccgtcgggca gccagcccag gccgcagtcg
     8821 ccggtgtatc cggagtcgag caggtgcacg gcggcgatcg gctggaagcg gatcgagccg
     8881 ctcagcgcct ccccttccgg atcggcggcg cgtccctgcg cctcgaggcg cgtgccgccg
     8941 tcctcccgga tcgacgtcag ggtcaggctc ggctggcagg catcccccgc cccgtcggcg
     9001 ttgccgtccg cctggtccgg attggcgcgc gccgggcagt tgtcgcaggc gtcgccccgg
     9061 ccgtcggcgt cggcgtcctg ctgccccgga ttcgacaggg ccgcgcagtt gtcgcacgca
     9121 tcgccgcggc cgtccgcgtc ctggtccgtc tgcgaggcgt tcgcgaccgc cggacagttg
     9181 tcgcacgcgt cgccccggcc gtcgccgtcc ccctcctgct gccccggatt cggcagcgac
     9241 gggcagttgt ccggggcgca gacgttcccc gggagcggcg gatcgccggc gccgtcgccg
     9301 tcgatgtcgg tgcagggatc gcaggcgttg ccggcaccgt cgagatcggt gtcctgctgc
     9361 cccgggttcg agacggcggg gcaattgtcg agcgcgcagg tgttggccgg gtacccggcg
     9421 ttcccgaacc cgtcgcgatc gaagtcggtg caggtgtcgc aggcgtcccc cacgccgtcg
     9481 tcgtcggaat ccgtcgggca gccgacgtac accgaccatg tcgcacggtc gaggttcgcg
     9541 ccggtcttcg cgggcttgac gaaattgacg tccgcctc
//
LOCUS       DASRIQ010000138        12470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2929144, whole genome shotgun sequence.
ACCESSION   DASRIQ010000138 DASRIQ010000000
VERSION     DASRIQ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2929144"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFD06_03270"
     CDS             complement(<1..186)
                     /locus_tag="VFD06_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02588.1"
                     /translation="MRSLPNSASRLRPFLSVAAATLGACLTVLAADPPALMNYQGVLR
                     DASDLPLSGSYDVTFRLY"
     gene            complement(205..2808)
                     /locus_tag="VFD06_03275"
     CDS             complement(205..2808)
                     /locus_tag="VFD06_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02589.1"
                     /translation="MMRTDLLVVMAAAAFLSPGLPLAADSRAMNYPPDVIAQFDALSH
                     RPDPLGFVTAFPPEPTECMHFQGMVRRQADDGTTYFYISRSANIPVLPDGLFCEFGET
                     AGDLRVVRMWSRDENGERLRSNRLQRGVNLTLTAPPIDDQEVNLIRFDGNYGWPKYRH
                     PGGMQLIGQVLALGLEKPYDDETLPPWQVLFLDVRQPELPLPLGAFAPEDVTLQTAIV
                     GITTMPDDHYLMVVTGGDNHVIGFYRSQFTNLVDFYSAQPRTLTEGWDHVDTWEAAPI
                     IRIPLPGGGTYVENPLSPDEEYAGQNWPGDPSHQTFQFLREGGRDGTLYVAGALGDIF
                     GDDDRLDLYRLDCEAGPNCAQGQIRLKWIASRLKSTRPNADPSAYGSFETANLAAAST
                     FYVSPSGELLFYATEHANQGPDGSVRFGEWRHTDLVRPGSPTFSPTVDLFEHPFDALE
                     GGAVVLRGFALPPVTKAFVQLFADPGFHGRYVVVEYDDWARDDFDNFHRLDYAIDILS
                     PTPDAPVDDGFSDDATSWRWFAPSGCTLRVNDHQFGHPDFPGPSTLELPGTGALSICP
                     DLRDGSEVCGPANMNDDITSVEFLPDCDDYYGAPMTLEWDLDLDGVCESAGSLVVFST
                     LNFDGPGTVQVPVRAVHPRDGRTTLTSGEVAVYNVAPAIGSFRVSDPLGREIGTEVPF
                     VVQGLPITVTAQFTDPGRPDHQTADIGWGDGVVESESAFGSFSDAFGGRTGALRHSHT
                     HALAGSYRVGLSVTDDDGGPAGAAGVVRVVSPAGLVTEAIGLIDARLAAAADPRLIKA
                     LQKARKALAGSPEGHGSNGVIDKLGAGATRDTLVMLRGAIDDLRAAQALGADVGAIVL
                     LLEQLVLALSL"
     gene            2923..3633
                     /locus_tag="VFD06_03280"
     CDS             2923..3633
                     /locus_tag="VFD06_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN02590.1"
                     /translation="MSDRFQTTHWSLVLAAARGGEGAAAALEWLCGAYWYPLYAFIRR
                     QGHDADAARDLTQSFFLHVIEKDALKAIDPRLGKFRAFLLASVKNFMANEREREGAQK
                     RRADHPAFRLSLEDAEPRYLNERPADLSPEELFERRWARAILDRAVRRLEKEHAAASR
                     GEVFARLRGHLTGEEAAYDRMAEELGMTEGALRAAVHRMRRRLGTLLREEVAQTVASP
                     ADIEDEIRQLLQAAGRGS"
     gene            3658..6411
                     /locus_tag="VFD06_03285"
     CDS             3658..6411
                     /locus_tag="VFD06_03285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN02591.1"
                     /translation="MADPDRCSTCGGPLASEGATPPICIPCAFTLALKEVADGAPDAA
                     DGPIHEAAAVERIGPYRLERILGEGGMGVVWQAAQEEPVRRQVALKRLKLGMDSAQVL
                     ARFESERQALALMSHAHIAQVFDAGRTPQGQPYFAMEYVDGTWLTRHCDAGRLTVSQR
                     LDLFLLVCEAIQHAHQKGVIHRDIKPSNILVQTGKDRPTPKVIDFGVAKAIGAHLTER
                     TLVTQIGQTIGTPEYMSPEQSGPAGFDVDTRTDVYALGVVLYELLTGRLPFEAPEGDP
                     DELRRRIREDEPKRPSVRVAGSGPEDADVARARGTDPAGLARRLRGDLDWITLKALAK
                     DRAQRYGSAEDLAEDLRRHLAGLPVLAGPPTAAYRMSKFVRRHLVAVAAGSTMLLLLA
                     TTAVGMTIQAGRVARERDRANQEAKTAGAALEFLTDIFQLSDPEQARGETITAREILD
                     KGARDVERDLKAAPDVQARLILAIGRSYKNLGLYDRAEPLVRKAVGLRQDRSGPRVPG
                     RLEASLELADILRIREEYDDAEATARDTLRALEATVGADDPETANAHLVLGDTLFDRG
                     RYDEAEPHLRTALDVWTRTLGPEDRRTLHAAALFGYFLSDRGRYDEADALLRRALEDQ
                     RRAFGADSPHSIIMLTYLAQVSLRRGQLSEGEAQYREALGLARRVFGEDHLETHRVAY
                     SLGRHLLATERLDEAEALIGAALAGRRRLLGPRHQAVFVVLADWGSVQLNRGHLEEAE
                     KAVREALDGVREISGEDHPQTLRLLNLLSIVLKERGRLEEAETYARSALEGNSRVFGP
                     AHTYTLTALGNLGYLIAQRGRHTEALPLLEEALQGQRKALPARHLNIGITLSKYGYCL
                     VGLRRFEEAEAALLEAHDIVLAEVGASHNQAQGVIKNLIALYETWGKGESAARWRAAR
                     TSV"
     gene            6595..7053
                     /locus_tag="VFD06_03290"
     CDS             6595..7053
                     /locus_tag="VFD06_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02592.1"
                     /translation="MIPSTVRRVPLHTIGSVNEQIRKRTEESVARTAAQGPEAIDRRL
                     EELDWEWDIDRAVEAKLASMILIGSALGVLHDRRFFAIPAIASSFLLLYAIQGWCPPL
                     ALLRRAGFRTATEIDHERYALKVLRGDFKTNGAPTGERLAATLAVEAARA"
     gene            complement(7091..7621)
                     /locus_tag="VFD06_03295"
     CDS             complement(7091..7621)
                     /locus_tag="VFD06_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN02593.1"
                     /translation="MTLSALARRFALTTAVAATIGIIPATSGSTPAPGAGATSPSGAA
                     SDEAALRARHARVIQAHRESNPDMLCEDVSPDFVQANRGEIKRPTIEERRARFAAYLG
                     STIFEEYRDLVEPIVHVSKDGTLGWVIVQVKARGVQTTETGAKEPLEFVSAWIELYEK
                     RDGRWLAAGNVSNFKP"
     gene            complement(7630..8238)
                     /locus_tag="VFD06_03300"
     CDS             complement(7630..8238)
                     /locus_tag="VFD06_03300"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN02594.1"
                     /translation="MSAWSPGVVTRPLPFVWPYALLFWGVHLWAFWPEFRIIREARRA
                     ATAQDAKSVQVITFGMWIGYFAAYPLAWVPALQIRTHRVAVFLAGTALIVAGSLLRRH
                     CWRMLGTSFTGDVRAKAGQEVVTRGAYAILRHPSYTAATLMNTGVGIALGSWGSAALL
                     LVVSLLVYGYRITVEERVLLAAIGEPYARFVAARKRLIPFIY"
     gene            complement(8235..8825)
                     /locus_tag="VFD06_03305"
     CDS             complement(8235..8825)
                     /locus_tag="VFD06_03305"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZN02595.1"
                     /translation="MRSDDLYSLPLGLPVPVDDGACDHLPGRPWPVLRLPSTSGGTVA
                     LAEVDTDWQVLYLYPRTGQPDRDPPGGVRRWDAIPGSRGCTPQACAYRDLHAELRDLG
                     CRIFGISTQDTDYQREAAGRLHLPFPLLSDGGLELTHALRLPTFEVAGMTLLKRLTLI
                     GRRERIERCFYPVFPPDADAGNVLRYIHDRVHGENQ"
     gene            complement(8822..9802)
                     /locus_tag="VFD06_03310"
     CDS             complement(8822..9802)
                     /locus_tag="VFD06_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZN02596.1"
                     /translation="MNAITYCDYGTSEVLRLEPVEKPVAADDQVLVRVRAASVNPLDW
                     HYMRGTPYFMRLDAGLRKPAVTRLGVDFAGTVEAVGRSVTTLKPGDEVFGTRNGAFAE
                     YVAVRAERVATKPAAMTFEQAASIPVAAVTALQAVRDKGHLQSGEKLLINGASGGVGT
                     FMVQIAKTLGAQVTGVCSTRNLEMVRSLGADHVIDYTQEDFSKRDERYDLIVDNVGNR
                     SLSDFRRVLAPKGRYVLVGGGGPNDGRVIGPLGKVLYALVLSWFVPQQMGMMLADTNK
                     EDLGILAGLIESGKVTPVIDRRYPLSEVPEAITYLETGRARGKVIITIDE"
     gene            complement(10017..10787)
                     /locus_tag="VFD06_03315"
     CDS             complement(10017..10787)
                     /locus_tag="VFD06_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02597.1"
                     /translation="MSRVTAPAIVAVSLVLCVISTVAYLLIAPLFFQSIPWLGAVSLS
                     VWLWERIPGWVYNILVRGLFQIALFGFLVAMTVSLVAGPRALRARLRRRGMTLSLCGL
                     LLAAVYCAGPTIWYARSRRTMEELQASGKILMANLRESLEAGTIPEPQRERMWQLYAG
                     AMYRDEGIEVEIPDATGRLVAWQPSDSDIAVRRVHLEMLERLRPPTGQVKGSVAWVAL
                     CLGVGLLWPLRRRTTAGDASTAQSAPPHNPGTGTAGGE"
     gene            complement(10793..11299)
                     /locus_tag="VFD06_03320"
     CDS             complement(10793..11299)
                     /locus_tag="VFD06_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN02598.1"
                     /translation="MTITPRFPFAVFAVLTLSMVLTLAIAPAGAAPPKPLPADVGAGR
                     VAWFDITTTDLPRSKAFYGGLFDWKFLPVEGTDQAVEIASRGTSIGTIRVAEGAISGF
                     NGVIYVQVSDIQAACARAKTLGATIAAGFPFNLPTGIGAIAVLRDPAGHPLGLYSRTP
                     LPEAPTSK"
     gene            complement(11326..11904)
                     /locus_tag="VFD06_03325"
     CDS             complement(11326..11904)
                     /locus_tag="VFD06_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02599.1"
                     /translation="MTAPAAETRTSPVLRTLDEQRLEFVRGRYLAMPIAGTIVWTIVG
                     IAGAALPIGLASWVLFIATGSTFGVGILVARVIDEDLLGKNRASNWFDTLFFHTVMMA
                     WLVFAIAIPFFMIEPTSLPLTVGVLAGLMWIPFSWMIGHWIGLFHGIARTVLVLAAWY
                     LFPTQRFVAIPAVIVVIYLITIAVLLKRSRPA"
     gene            complement(11901..12299)
                     /locus_tag="VFD06_03330"
     CDS             complement(11901..12299)
                     /locus_tag="VFD06_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZN02600.1"
                     /translation="MKKSKPAESPDAYVAALTGWRRSCVEALRKVVRRAAPLEEVIKW
                     RNLVYLAKGPVLVIRAEESRVLFGFWRGKRLRDIEPRLKPGGKYEMATLSLREGETVD
                     PATVHRLTREAVALNRRLGDPTLAARREKK"
BASE COUNT         2038 a         4227 c         4379 g         1826 t
ORIGIN      
        1 atagagtcgg aaggtgacgt cgtagctgcc gctgagcggc aggtcgctcg cgtcgcgcag
       61 gacgccctgg tagttcatca aggcgggcgg gtcggcggcc agcacggtca ggcaggcccc
      121 gagggtcgcc gcagccacgg acaggaatgg acgcagacgg ctcgcagaat tcggcaggga
      181 acgcatggcc ttttccccct ttctctagag tgacagggcg aggacgagct gctcgagcag
      241 caggacgatc gcgccgacgt cggcgcccag ggcttgcgcc gcgcgcaggt cgtcgatcgc
      301 cccgcgcagc atgacgagag tgtcccgcgt ggctcccgcg cccagcttgt cgatcacacc
      361 gttgctgccg tggccctccg ggctgccggc gagcgcctta cgcgccttct gcagcgcctt
      421 gatcaggcgc gggtcggcgg cggcggcgag acgggcgtcg atgaggccga tcgcttcggt
      481 gaccagcccg gccggcgaga cgacgcgcac cactccggcg gcgccggcag ggccgccgtc
      541 gtcatcggtg accgacagcc cgacgcggta gctccccgca agcgcgtgcg tatggctgtg
      601 ccggagcgcg cccgtgcgcc cgccgaacgc gtcgctgaag gagccgaagg cgctctccga
      661 ttccacgaca ccgtctcccc agccaatgtc ggcggtctgg tggtccgggc ggcccggatc
      721 ggtgaactgg gccgtgacgg tgatcggaag cccctgaacg acgaacggga cctcggtccc
      781 gatctcccgc ccgagggggt cggagactcg aaacgacccg atcgccggcg cgacgttgta
      841 cacggcgact tcgccagacg tcagggtcgt gcggccatcg cgaggatgca ccgcccggac
      901 ggggacctgg acggtgccgg gaccgtcgaa gttcagggtg ctgaacacga cgagggagcc
      961 ggccgattcg cagactccgt cgaggtcgag gtcccactcg agcgtcatcg gcgcgccgta
     1021 gtagtcgtcg caatcgggca agaattcgac cgaggtgatg tcgtcgttca tgttggcggg
     1081 accgcagacc tcggagccgt cgcgcagatc ggggcagatg ctcagcgcgc ccgtcccggg
     1141 cagctcgagg gtgctcggcc ccgggaagtc cggatgcccg aactggtgat cgttgacgcg
     1201 cagcgtgcag cccgacgggg cgaaccacct ccaggaggtg gcgtcgtcgc tgaacccgtc
     1261 gtcgaccggc gcgtcgggag tggggctgag aatgtcgatc gcgtagtcca gccggtggaa
     1321 attgtcgaag tcgtccctgg cccagtcgtc gtattcgacc acgacgtagc ggccgtggaa
     1381 gccagggtcg gcgaagagct gaacgaaggc cttcgtcacc ggcggaaggg cgaagcccct
     1441 cagcacgacc gccccacctt ccagggcatc gaacggatgc tcaaagaggt ccacggtcgg
     1501 tgagaaggtc ggactgcccg ggcgcacgag gtcggtgtgg cgccactcgc cgaaccgcac
     1561 gctgccgtcc ggcccctggt tcgcgtgctc ggtggcgtag aagagaagct cgccgctcgg
     1621 cgacacgtag aacgtcgagg cggcggccag attggcggtc tcgaaggagc cgtaggcgct
     1681 cggatcggca ttcggtcggg tgctcttcag gcggctggcg atccacttca gccggatctg
     1741 gccctgcgcg cagttagggc cggcttcgca atcgaggcga tagaggtcga gacggtcgtc
     1801 gtcgccgaag atgtcgccga gggctccggc cacgtacagc gttccgtccc tgccgccttc
     1861 ccgcaggaac tggaaggtct ggtgcgacgg atcgcccggc cagttctggc cggcgtactc
     1921 ctcgtcgggc gagagcggat tctcgacgta ggtgccgcct cccggcagcg ggatccggat
     1981 gatcggcgcc gcctcccagg tgtcgacgtg atcccatcct tccgtgagcg tcctgggctg
     2041 cgccgaatag aagtcgacga ggttcgtgaa ctgcgagcgg tagaagccga tgacgtggtt
     2101 gtcgccgccg gtcacgacca tgagatagtg gtcgtcgggc atcgtcgtga tcccgacgat
     2161 ggccgtctgc agcgtgacgt cctcgggggc gaaggcgccg agcggcagcg gaagctccgg
     2221 ctggcgcacg tccaggaaca ggacctgcca aggcggcaac gtctcgtcgt cgtagggctt
     2281 ctcgaggccg agcgcgagga cctggccgat gagctgcatc cctccgggat ggcgatactt
     2341 cggccagccg taattgccat cgaatcgaat gaggttgact tcctgatcgt cgatcggcgg
     2401 cgccgtgagg gtgagattga ctccgcgctg cagccggttg ctgcgcaagc gctcgccgtt
     2461 ttcgtcgcgc gaccacatgc gcacgacgcg cagatcgccc gcggtctctc cgaattcgca
     2521 gaagagcccg tcgggcagga cggggatgtt ggcgctgcgg ctgatgtaga aataggtcgt
     2581 tccgtcgtcg gcctggcgcc gcaccatccc ctggaagtgc atgcattcgg tcggctcggg
     2641 aggaaaggcc gtcacgaagc cgagcggatc gggccggtgc gacaaggcgt cgaactgcgc
     2701 gatcacgtca gggggatagt tcatcgcgcg ggaatccgcg gcgaggggca gccccggcga
     2761 gaggaatgcc gcagcggcca tcacgaccag gagatcggtt cgcatcatga caggtccctc
     2821 cgttctcttc cgtcaaacgg atcgcgcgcg acctgttatg ggggggggca cggagggctg
     2881 tgctagactc gcgcgcacca gagacaatcg acgaacacga cggtgtctga ccgcttccag
     2941 accacgcact ggagtctcgt gctggcggcc gcccgcggag gagaaggagc ggcggcggcg
     3001 ctcgagtggc tctgcggcgc ctactggtat ccgctctacg ccttcatccg ccggcagggg
     3061 cacgacgccg acgccgcccg ggacctcacg cagtcgttct tcctgcacgt catcgagaaa
     3121 gacgccctca aggcgatcga tccccgactg gggaagttcc gggcctttct gctcgcgtcg
     3181 gtcaagaact tcatggcgaa cgagcgggag cgggaaggag cgcagaaacg gcgcgcggat
     3241 cacccggctt tccgtttgag cctggaggac gcggagccgc gctatctgaa cgaacggccg
     3301 gccgacctca gccccgagga gctgttcgag cggcgctggg cgagggcgat cctggatcgg
     3361 gccgtgcggc gtctggagaa agagcacgcg gcggcgagcc ggggcgaagt cttcgcccgg
     3421 ctgcgcgggc atctgaccgg cgaggaggcg gcctacgatc gcatggcgga ggagctcggg
     3481 atgaccgaag gggcgctgcg ggccgctgtc cacaggatgc gacggcgcct ggggacgctg
     3541 ctgcgcgaag aggtcgcgca gaccgtcgcg agccccgccg acatcgagga cgaaatccgg
     3601 cagctgctgc aggccgccgg acgcgggagc tgagccgttc ccggcggagg aagcgtgatg
     3661 gccgatccgg atcgatgctc cacctgcggc gggccactgg cctctgaagg agcgacgcct
     3721 ccgatctgca tcccctgcgc cttcacgctc gccctcaagg aagtcgctga cggcgcgcca
     3781 gacgccgccg acggcccgat ccacgaggca gccgcggtcg agcggatcgg cccctaccgg
     3841 ttggagcgaa ttctgggcga aggcgggatg ggggtggtgt ggcaggcggc ccaggaggag
     3901 ccggtccgcc gccaggtcgc cctcaagaga ctcaagctcg ggatggacag cgcccaggtc
     3961 ctggcgcgct tcgagtcgga gcgccaggcg ctggcgctga tgagccacgc ccacatcgcc
     4021 caggtcttcg atgccggccg cacgccccag gggcagccgt acttcgccat ggagtatgtc
     4081 gacgggacct ggctgacgcg ccactgcgac gcaggccggc tgacggtgtc acagcggctc
     4141 gacctcttcc tcctggtctg cgaggcgatc cagcacgcgc accagaaggg ggtcattcac
     4201 cgcgacatca agccgtccaa catcctggtg cagaccggca aggatcgtcc gacccccaag
     4261 gtcatcgact tcggggtggc gaaggcgatc ggggcgcacc tgaccgagcg gacgctcgtc
     4321 acccagatcg gccagacgat cgggaccccc gagtacatga gtcccgagca gtcaggcccg
     4381 gcggggttcg atgtcgacac gcgcaccgac gtctacgctc tcggggtcgt gctttacgag
     4441 ctgctgacgg gccgactgcc gttcgaggcg cccgaaggcg atccggacga gctgcggcgg
     4501 cgcatccgcg aggacgagcc gaagcggccc agcgtccgcg tggcgggctc cggaccggaa
     4561 gacgcggacg tcgcgcgtgc acgcggcacc gatcccgcgg gcctcgcgag gcggctgaga
     4621 ggcgatctgg attggatcac cctcaaagca ctcgccaagg atcgggctca gcggtacggc
     4681 tcggcggagg atctggcgga ggacctgcgg cgccacctgg cggggctgcc ggtcctggcc
     4741 ggccctccga cggcggccta tcggatgtcg aagttcgtcc gccggcacct cgtcgcggtc
     4801 gccgccggct cgacgatgct cctgcttctg gcgacgaccg cggtcggcat gacgatccag
     4861 gccggccgcg tggcgcgcga gcgcgaccgc gccaatcagg aggcgaagac ggccggggcg
     4921 gcgctggagt ttctcaccga catcttccag ctgtcggacc ccgagcaggc ccggggggaa
     4981 acgatcaccg cgcgcgagat cctggacaag ggcgctcgag acgtggagcg cgacctcaag
     5041 gcggcgcccg acgtccaggc ccgactgatc ctggcgatcg gccgcagcta caagaacctc
     5101 ggcctgtacg atcgcgcgga accgctggtg cgaaaggccg tcggcctgcg ccaggatcga
     5161 tccggcccca gggttcccgg gcgtctcgaa gcatcgctcg agctggcgga catcctgcgc
     5221 atccgcgagg agtacgacga cgcggaggcg acggcgcgcg acacgctgcg cgccctcgaa
     5281 gcgacggtcg gcgccgacga tccggaaacc gcgaacgcgc acctcgtcct cggcgacacg
     5341 ctcttcgatc gcggccggta cgacgaggcc gagcctcatc tccgcacggc gctcgatgtc
     5401 tggacccgga cgctcggtcc cgaggatcgc aggacactcc acgccgcggc gctctttggg
     5461 tacttcctct cggatcgcgg ccgctacgac gaggccgacg cgcttctgcg ccgtgccctg
     5521 gaggaccagc gacgagcctt tggcgccgac agcccgcact cgatcatcat gctcacctac
     5581 ctggcgcagg tctcgctgcg acggggccag ctcagcgagg gcgaagcaca gtaccgggaa
     5641 gcgctcggcc tcgcgcgacg cgtcttcggc gaggatcatc tcgagaccca ccgtgtggcg
     5701 tacagcctgg ggcggcacct ccttgcgacg gaacgactcg acgaagccga ggcgctgatc
     5761 ggcgcggctc tggcagggcg ccgccgcctc ctggggccgc gccaccaggc ggtcttcgtg
     5821 gtcctggccg actgggggag cgtgcagctc aaccgcgggc atctcgagga agcggagaag
     5881 gcggtacgcg aggcgctcga tggcgttcgc gagatatcgg gagaggacca cccccagacg
     5941 ctgcgccttc tcaatctcct ctccatcgtg ctcaaggaaa ggggtcgcct ggaggaggcg
     6001 gagacctatg cccgctccgc gctggagggg aacagccgcg tcttcgggcc ggcgcatacc
     6061 tacacgctca cggcgctcgg gaacctcggc tatctgatcg cgcagagggg ccggcacacg
     6121 gaggcgttgc cgcttctcga ggaggccctg caggggcagc gcaaggcgct ccccgcccgc
     6181 catctcaaca tcggcatcac actgagcaag tacggctatt gcctcgtcgg cctgcgccgc
     6241 ttcgaggagg ccgaggcggc gctgctcgag gctcacgaca tcgtcctggc ggaggtcggc
     6301 gcgagccaca accaggcaca gggagtcatc aagaacctga tcgccctgta cgaaacctgg
     6361 ggcaagggcg agagcgccgc gcgctggcgc gcggcccgca cctcggtctg acgtcctcac
     6421 cctgccgctc ccccggcggc tgcgggccgc gtaagtccag gtttcgcgtc cgggtcgaac
     6481 atttccccac agccggttcc gtagaactgc ggcgggtcgc cgcccgcggt cgatgcgacc
     6541 atggcacgca ttcattcttg cgtgcattcg atgccgcatt ggaggtcgat cctcatgatt
     6601 ccttccactg tccgccgcgt tcctctccac accatcgggt cggtcaacga acagattcgc
     6661 aagcggaccg aagagagcgt cgcgcgcacc gccgcccagg ggcccgaggc gatcgatcgc
     6721 cgtctggaag agctggattg ggaatgggac atcgatcggg ccgtcgaggc gaagctggcc
     6781 tcaatgatcc tgatcggctc ggccctcggc gtgctccacg atcggcgctt cttcgccatt
     6841 ccggcgatcg cctcgagctt cctcctgctg tacgccatcc agggctggtg cccgccgctg
     6901 gcgctcctgc gccgcgccgg gttccggacc gccaccgaga tcgaccacga gcgctacgcg
     6961 ttgaaggtgc tgcgcggcga cttcaagacc aacggagcgc cgaccggcga gcgcctggcc
     7021 gccaccctcg cggtcgaggc ggcgcgcgcc tagcggcgct cgggcgccca gagcccaggg
     7081 ctttcggagg ctagggcttg aagttggaga cgttgccggc ggcgagccag cggccgtcgc
     7141 gcttctcgta cagctcgatc caggcgctga cgaactccag cggctccttc gcgccggtct
     7201 cggtcgtctg caccccgcgc gccttcacct ggacgatgac ccagccgagc gtgccgtcct
     7261 tcgagacgtg gacgatcggc tcgaccaggt cgcggtactc ctcgaagatc gtgctcccga
     7321 ggtaggccgc gaaccgggcg cggcgctcct cgatcgtggg gcgcttgatt tcgccgcggt
     7381 tcgcctggac gaagtccggc gagacgtcct cacagagcat gtcgggattg gactcccggt
     7441 gcgcctgaat caccctcgcg tgccgcgccc gcagggccgc ctcgtccgaa gcggcgccgg
     7501 agggggacgt cgcgccggcg cccggagccg gggtcgagcc cgacgtcgcc gggatgatgc
     7561 cgatcgttgc cgccaccgcg gtggtgagcg cgaagcgccg agccagagca gagagtgtca
     7621 tcgtccgcct caatagatga atggaatcag ccgcttgcgc gcggccacga accgcgcata
     7681 cggctcgccg atcgcggcca ggagcacccg ctcctcgacc gtgatgcggt agccgtacac
     7741 cagcaaggat acgacgagca ggagcgccgc cgagccccac gaccccaggg cgatcccgac
     7801 ccccgtgttc atcagcgtgg ctgccgtgta ggaagggtgt cggaggatcg cgtaggcgcc
     7861 gcgcgtgacc acctcctgcc ccgccttcgc ccgcacgtcc ccggtgaacg atgtgccgag
     7921 catccgccag cagtgacgtc gcagcagact cccggcgacg atgagcgccg ttccggccag
     7981 gaagacggcg acgcgatgcg ttcggatttg cagcgcgggg acccaggcca ggggataggc
     8041 ggcgaagtag ccgatccaca tcccgaaggt gatcacctgg accgacttcg cgtcctgggc
     8101 cgtcgccgcg cgccgcgcct cccggatgat ccggaactcc ggccagaagg cccacaggtg
     8161 cactccccag aagagcaggg catacggcca gacgaacggc agcggccgcg tcacaacacc
     8221 aggcgaccac gcgctcattg attctctcca tggacgcggt cgtggatgta ccggaggacg
     8281 ttgccggcgt cggcatcggg cgggaacacc gggtagaagc agcgctcgat ccgctcccgg
     8341 cggccgatga gcgtcaggcg cttgaggagc gtcatgccgg caacctcgaa cgtcggcagc
     8401 cggagcgcgt gcgtcaactc gagcccaccg tcgctcagga gcgggaaggg caggtgcagc
     8461 cgcccggccg cctcccgctg atagtcggtg tcctgggtgc tgatgccgaa gatgcggcag
     8521 ccgagatcgc gcagctcagc gtggaggtcg cggtaggcgc acgcctgcgg ggtgcagccg
     8581 cgcgagcccg ggatggcgtc ccaccggcgc acgccgccgg gcgggtcccg gtccggctgg
     8641 ccggtgcgcg gatagaggta gagcacctgc cagtcggtgt ccacttcggc cagcgccacc
     8701 gtcccgccgc tggtggaggg gagacggagc accggccacg gccttccggg aagatgatcg
     8761 caggcgccgt cgtccaccgg caccggcagc ccgagcggca ggctgtacag gtcgtcggat
     8821 ctcattcgtc gatcgtgatg atcaccttcc cccgcgcgcg tccggtctcg aggtacgtga
     8881 tcgcctccgg gacttcgctg agagggtacc gccggtcgat caccggcgtc accttgccgg
     8941 actcgatgag cccggccagg atgccgaggt cctccttgtt cgtgtccgcc agcatcatcc
     9001 ccatctgctg cggcacgaac caggacagga cgagcgcgta cagcaccttg ccgagcgggc
     9061 cgatgacgcg gccgtcgttc ggcccgcccc cgccgacgag gacgtagcgc cccttcggag
     9121 cgaggacgcg ccggaagtcg gacagcgacc ggttcccgac gttgtccacg atgaggtcgt
     9181 aacgctcgtc ccgcttcgag aaatcctcct gcgtgtagtc gatcacatgg tcggcgccga
     9241 gggagcggac catctcgagg ttcctggtgc tgcagacgcc ggtgacctgc gcgccgagcg
     9301 tcttggcgat ctggaccatg aaggtgccca ccccgcccga ggcgccgttg atgagcagct
     9361 tctcgcccga ttggagatgt cccttgtccc gcacggcctg cagggcggtc accgccgcga
     9421 cgggaatgga ggccgcctgc tcgaacgtca tggcggccgg cttcgtcgcg acgcgctccg
     9481 cgcgcacggc gacgtactcg gcgaaggcgc cgttgcgcgt gccgaagacc tcgtccccgg
     9541 gcttgagcgt cgtgaccgaa cggccgaccg cctcgaccgt cccggcgaaa tcgaccccga
     9601 gccgcgtcac cgccggcttg cgcaacccgg catcgagccg catgaaatac ggcgtgccgc
     9661 gcatgtagtg ccagtcgagc gggttgaccg acgccgcgcg cacccgcacg aggacctgat
     9721 cgtccgcggc gaccggcttc tccaccggct cgaggcgcag cacctcggac gtgccgtagt
     9781 cgcagtaggt gatcgcgttc atcgggtcac gcggcggccc ggccggcggc ttgtcgcagg
     9841 cgttcgtcga tcggaaccag gcgatcgatc cgaacacgac gaccccgatc aggaggacgc
     9901 cgccccccca caggacgatc cgccggaaga tgccgagcgc gcgcttgagc cccatggtcc
     9961 cgtcccctcc tgggcgcggg acaaccgcgc cgcctcacga gcgactctgg gggagcctac
    10021 tcccctccgg ccgtcccggt tccgggattg tgaggcggag cgctttgcgc cgtggacgcg
    10081 tcaccggcgg tggtgcggcg ccgcaacggc cagagcaggc cgacaccgag gcagagcgcc
    10141 acccaggcca ccgacccctt gacctgcccg gtgggcgggc gcagccgttc gagcatttcg
    10201 agatgcacac gcctcacggc gatgtcagaa tcggatggtt gccaggcgac caggcgcccg
    10261 gtcgcatcgg gaatctcaac ctcgatccct tcgtctcgat acatcgctcc cgcatagagc
    10321 tgccacatgc gctcccgctg gggttccgga atggtccccg cttcgaggct ctcccgcaga
    10381 ttcgccatca ggatcttccc ggaggcctgc agttcctcca tggtgcgccg cgaacgggcg
    10441 taccagatcg tcggtccggc gcagtacacg gcggcgagca gcaaaccgca cagggaaagg
    10501 gtcatacccc ggcgccggag ccgggcgcgc agcgcccgcg gtccggcgac gagcgagacc
    10561 gtcatcgcca cgaggaagcc gaacaaggcg atctggaaga ggccgcgcac caggatgttg
    10621 tagacccatc cggggatgcg ctcccacagc cacacggaga gcgagaccgc gccgagccaa
    10681 gggatgctct gaaagaacaa cggggcgatc agcaggtagg cgacggtgct gatgacgcac
    10741 aggaccagcg agaccgcgac gatggcgggc gcggtgacgc ggctcatcgc tttcattttg
    10801 atgtgggtgc ttccggcaga ggtgtcctcg agtacaggcc gagcgggtgg ccggcgggat
    10861 cgcggaggac ggcgatggcg ccgatgccgg tcggcaggtt gaacgggaag ccggccgcga
    10921 tggtcgcgcc cagcgttttc gctctcgcgc aggccgcctg gatgtcggac acctggacgt
    10981 agatgacgcc gttgaagccg ctgatcgccc cctcggcgac ccggatcgta ccgatcgacg
    11041 tgccgcgcga ggcgatctcg accgcctggt cggtcccctc caccggcagg aacttccagt
    11101 cgaagaggcc gccgtagaac gccttcgagc gcggcaggtc ggtcgtggtg atgtcgaacc
    11161 acgcgacacg tcccgcgccg acgtccgccg gaagcggctt cggtggcgcc gctcctgccg
    11221 gcgcgatcgc cagcgtcagg accatcgaca gagtcaggac cgcgaagacc gcgaacggga
    11281 aacggggagt gatcgtcatg ggtgcctcca ccagatgatg agcctctagg caggtcgcga
    11341 acgcttcagc aggacggcga ttgtgatgag gtagatgacg acgatgaccg cggggatggc
    11401 gacgaaacgc tgcgtcggaa agaggtacca cgccgcgagc acgaggaccg tccgggcgat
    11461 gccgtggaac aggccgatcc agtggccgat catccacgag aaggggatcc acatcaggcc
    11521 ggcgaggacc ccgaccgtca gcggcagcga ggtcggctcg atcatgaaga acgggatggc
    11581 gatcgcgaag accagccacg ccatcatgac ggtgtggaag aagagcgtgt cgaaccagtt
    11641 ggacgccctg ttcttcccca gcaggtcttc gtcgatcacg cgggccacga ggatcccgac
    11701 gccgaaggtc gagccggtcg cgatgaacag cacccaggag gccagcccga tcggcagggc
    11761 cgcgccggcg atcccgacga tcgtccagac gatggtgccg gcgatcggca tggcaagata
    11821 ccggccgcgg acgaactcca ggcgctgttc gtcgagggtg cggaggaccg ggctcgttct
    11881 cgtctctgcg gcaggtgccg tcatttcttc tccctcctcg cggccagggt cggatccccg
    11941 agccggcggt tcaacgccac ggcttcacgg gtcagacggt ggacggtggc gggatcgacc
    12001 gtctcaccct cgcgcagcga aagcgtcgcc atctcgtact ttcctccggg tttgaggcgc
    12061 ggctcgatgt cccgcaggcg cttcccccgc cagaagccga aaagcacgcg gctctcctcg
    12121 gcgcggatca ccaggacggg gcccttggcc agatagacga ggttgcgcca cttgatgact
    12181 tcctcgaggg gcgcggcgcg gcgtacgacc ttccgcagcg cttcgacgca cgatcgccgc
    12241 cagcctgtga gcgcggcgac ataggcgtcg gggctctccg ccggtttcga cttcttcatg
    12301 tcctgtgcag ccactcggaa tactgccgcc agtccttgtc gtagatggac gctctctctt
    12361 cgtccgtcag cgactcgatc cgggaccagc gcttcgtggc gagcgggtac tcggcttccc
    12421 cgaggatctc gcggtagtag ccggacgcct cccccagatg gccccgcagg
//
LOCUS       DASRIQ010000139        14247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_827153, whole genome shotgun sequence.
ACCESSION   DASRIQ010000139 DASRIQ010000000
VERSION     DASRIQ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 14247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14247
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827153"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /gene="secY"
                     /locus_tag="VFD06_03335"
     CDS             complement(<1..836)
                     /gene="secY"
                     /locus_tag="VFD06_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZN02601.1"
                     /translation="MIDAFKNIFSIPDLRKRVLFTFLMLAVYRVGGHIPVPGVNFEAL
                     ESLFRTQGGGLLGFLDLFSGGNLRRLTIFALGIMPYISASIILQLLTVVWPYLEKLSK
                     EGEVGRKKIVQYTRYGTVLLSLVQGLGIAFWVENLNQAGGGGGAIVPSPGWFFRIMTI
                     VTLTTGTAFIMWLGEQISERGIGNGISLIIYVGIVAGLPAAIATLWQDVSTGTLNPFM
                     LVLLLAVMVGVVAAIVYVERAQRKVPVQYARRVVGRRMYGGMQTHMPLRLNTGGVIPV
                     IFA"
     gene            complement(833..1279)
                     /gene="rplO"
                     /locus_tag="VFD06_03340"
     CDS             complement(833..1279)
                     /gene="rplO"
                     /locus_tag="VFD06_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZN02602.1"
                     /translation="MRPAPGSRRKRKRVGRGPGSGHGKTATRGNKGQLSRSGSTAQYF
                     GFEGGQMPLHRRIPKRGFTNIFRRQYAVINVGDLARLDGEITPERLVGEGMIKKLGAG
                     LKVLGDGELAKALVVRAHRFSRSAREKITAAGGTAEEIGAAKPATS"
     gene            complement(1443..1688)
                     /gene="rpmD"
                     /locus_tag="VFD06_03345"
     CDS             complement(1443..1688)
                     /gene="rpmD"
                     /locus_tag="VFD06_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZN02603.1"
                     /translation="MPETAKKKTAAKKPARARRAAGGTSLTIEQYRSGIGCNVTHKRV
                     LKSLGFRKIRQRVVRPDNPAVRGMVETIPHLLRIVES"
     gene            complement(1681..2187)
                     /gene="rpsE"
                     /locus_tag="VFD06_03350"
     CDS             complement(1681..2187)
                     /gene="rpsE"
                     /locus_tag="VFD06_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZN02604.1"
                     /translation="MNRIKADDLDLKDQVVHINRVTKVVKGGKNFRFSALVVVGDGRG
                     HVGYGAGKAKEVPAAIAKGVEKAKRNLILIPLKGHTIPHQVLGIFGAGQVLLKPAAPG
                     TGVIAGGAVRAVVESAGIQDVLTKSLGTTNPHNVVKATFAGLLMLRDPIAERRRRKGT
                     TGEVATDA"
     gene            complement(2197..2559)
                     /gene="rplR"
                     /locus_tag="VFD06_03355"
     CDS             complement(2197..2559)
                     /gene="rplR"
                     /locus_tag="VFD06_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZN02605.1"
                     /translation="MVNEVDRIRSREKRHARVRARVEGSGGRPRLCIYRSLGHIYVQA
                     IDDAQGRTLAHASTLELRKGKTPTGNVAAAKQVGTLIAERLKTLGCETVVFDRGGYLY
                     HGRVKAVADAARAAGLKF"
     gene            complement(2567..3124)
                     /gene="rplF"
                     /locus_tag="VFD06_03360"
     CDS             complement(2567..3124)
                     /gene="rplF"
                     /locus_tag="VFD06_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZN02606.1"
                     /translation="MSRIGRMPITLPAGVNVKTSGGAVTIEKGKASLTHRLPEGITCQ
                     VEGTKVTLARRDDDRRQRALHGLMRALVANAVKGVHDGFSKDLEIIGIGYKAQVNGRV
                     LQLALGYSHPVDYPIPDGIDIAVDKQTRLTVKGPDRQLVGQVAAEIRGLRAPEPYKGK
                     GIKYSTETIRKKAGKAGKTGAAAGG"
     gene            complement(3141..3533)
                     /gene="rpsH"
                     /locus_tag="VFD06_03365"
     CDS             complement(3141..3533)
                     /gene="rpsH"
                     /locus_tag="VFD06_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZN02607.1"
                     /translation="MNDPISDLLTRIRNAVRARHARTEMPHSRLKEAIAAILKEEGYI
                     ADVTTVEKGGFKVLRLRLRYDADGRPFVSGLERVSRQGKRVYAGHDAIPRVMGGIGLT
                     IMSTPRGMMSGSRAKKAGIGGEIVCAVW"
     gene            complement(3577..3762)
                     /locus_tag="VFD06_03370"
     CDS             complement(3577..3762)
                     /locus_tag="VFD06_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZN02608.1"
                     /translation="MATKAWRAAVKKPHKFAIRHRNRCKRCGRPRSYMRRFQMCRICF
                     RNLSLQGLVPGVTKSSW"
     gene            complement(3779..4321)
                     /gene="rplE"
                     /locus_tag="VFD06_03375"
     CDS             complement(3779..4321)
                     /gene="rplE"
                     /locus_tag="VFD06_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZN02609.1"
                     /translation="MPRLKSAYNATIARQMQERYAYPNVMAVPRLRKIVINMGVGEAS
                     QNIKLLDAAADQLGAIAGQKAIIRRARKSIANFKLRKGMPIGCTVTLRGDRMWEFFDR
                     LVSITLPRVRDFRGLPSRSFDGRGNYTVGLKDQLVFLEINYAKVDKARGMNVTIVTTA
                     KTDEEARNLLAALGMPFRRD"
     gene            complement(4386..4724)
                     /gene="rplX"
                     /locus_tag="VFD06_03380"
     CDS             complement(4386..4724)
                     /gene="rplX"
                     /locus_tag="VFD06_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZN02610.1"
                     /translation="MATTPRAVHVRRNDTVVVLSGKDKGRTARVLRVYPSKGRAIVEG
                     VNFHKKHTKPNPQRNIKGGILERETPIHASNLMVVCGECKKPTRVGRTILADGRKMRS
                     CRRCGGSLDK"
     gene            complement(4724..5092)
                     /gene="rplN"
                     /locus_tag="VFD06_03385"
     CDS             complement(4724..5092)
                     /gene="rplN"
                     /locus_tag="VFD06_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZN02611.1"
                     /translation="MIQMRTILEVADNSGAKRISCIHTRGRSINFYAGIGDVITANVK
                     EAIPDGTVKKGQVVKAVVVRMNKTHRRRDGTYIRFDTNAAVLINDAGEPIGTRIFGPV
                     ARELREKNFMKIVSLAPEVL"
     gene            complement(5178..5477)
                     /gene="rpsQ"
                     /locus_tag="VFD06_03390"
     CDS             complement(5178..5477)
                     /gene="rpsQ"
                     /locus_tag="VFD06_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZN02612.1"
                     /translation="MATEQQQAAAGTESAPRRRQEKVGVVVSAKMQKTVVVEVERLVM
                     HPLYQKYLRRRSRFMAHDEKGECRAGDRVSIRETKPLSRHKRWIVKSIVERAGAR"
     gene            complement(5500..5712)
                     /gene="rpmC"
                     /locus_tag="VFD06_03395"
     CDS             complement(5500..5712)
                     /gene="rpmC"
                     /locus_tag="VFD06_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009817515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZN02613.1"
                     /translation="MKARTLRDRSVNELREQEGTLREQIFKLRFQKATGQAENPQRIA
                     LARKELARVLTVLREKEGAAGEQPAR"
     gene            complement(5723..6133)
                     /gene="rplP"
                     /locus_tag="VFD06_03400"
     CDS             complement(5723..6133)
                     /gene="rplP"
                     /locus_tag="VFD06_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZN02614.1"
                     /translation="MLMPKKVKYRKQQRGRRRGKAWRGGTIAFGRYGLKATEAGWMTD
                     KQIEAARVAITRFIKRGGKIWVRVFPDKPITKKPAETRMGKGKGPPEGWVCVIKPGRV
                     LYEMEGVTSQEAREALRLASHKIGIRTMFCERGI"
     gene            complement(6211..6876)
                     /gene="rpsC"
                     /locus_tag="VFD06_03405"
     CDS             complement(6211..6876)
                     /gene="rpsC"
                     /locus_tag="VFD06_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZN02615.1"
                     /translation="MGQKVHPFGFRLGYNRTWQSRWYAEKNYASMLHEDLDLRGILKK
                     RLSHAGMARVDIERAANKLKITIHTSRPGIIIGRKGAEVDKLRDDLQKRTGREIFINI
                     MEIHKPELEAQLVAESVGLQIERRVAFRRAMKKAIESAVRFGAKGVKLRCSGRLGGSE
                     IARSEWYLDGQLPLHTLRADIDFGFAEARTTYGQIGVKCWIYKGELKGLTRPQDRLGL
                     AQI"
     gene            complement(6903..7481)
                     /gene="rplV"
                     /locus_tag="VFD06_03410"
     CDS             complement(6903..7481)
                     /gene="rplV"
                     /locus_tag="VFD06_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZN02616.1"
                     /translation="MLPAGLVGHVRLRYLGTSAQKARLVVDLIRGRRVEEALGTLRFT
                     PKAVAREILKALDSAVANARVKRPEVDVDRLVVGTAFVDGGPMLKRIRPAPMGRAFRV
                     QKRMCHVTLGLVETGDGRPAAAARRPSAKAEGAEPSAKEHAASAAPAGGSRTTHKRHA
                     ATRPARKEGPAGGPAKGKARMRGSGSKKGGGG"
     gene            complement(7495..7836)
                     /gene="rpsS"
                     /locus_tag="VFD06_03415"
     CDS             complement(7495..7836)
                     /gene="rpsS"
                     /locus_tag="VFD06_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZN02617.1"
                     /translation="MARSIKKGAFVDPVLRRRIEEMNRRSEKRVMKTWSRRSTIVPEM
                     VGHTIAVHNGKKFIPVYVSENMVGHKLGEFAATRTFRGHSRADSAAAAPAGAGPGGPG
                     GPPAAVVATPK"
     gene            complement(7846..8661)
                     /gene="rplB"
                     /locus_tag="VFD06_03420"
     CDS             complement(7846..8661)
                     /gene="rplB"
                     /locus_tag="VFD06_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZN02618.1"
                     /translation="MAIKNSRPTSPARRYYSTIVFDDLSDVRPLKRLAVGKRRTGGRN
                     NIGRIAVRHRGGGHRRLLRTIDFRRDKTGIPGKVATIEYDPNRSARIALVHYSDGDKR
                     YILAPLGLHVGQTVLAGPEADILPGNVLPLKAIPLGTGIHNIELKPGRGGQMVRSAGS
                     VAQLMARDAGWAQVKLPSGEIRKVLETCQATIGQVGNLDHENVSIGKAGRRRWLGIRP
                     TVRGVAMNPIDHPLGGGEGKTSGGRHPCTPWGVRERKTRNNKRTDKYIVRRRK"
     gene            complement(8670..8963)
                     /locus_tag="VFD06_03425"
     CDS             complement(8670..8963)
                     /locus_tag="VFD06_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZN02619.1"
                     /translation="MNRPQDVLIGPLITEKTQILKEGRNVLAFKVERRANKIEVKKAV
                     EALFKVKVASVRTENVHGKVKRVGRFEGKRPDWKKAYVTLKPGQKSIEYFEVV"
     gene            complement(8960..9586)
                     /gene="rplD"
                     /locus_tag="VFD06_03430"
     CDS             complement(8960..9586)
                     /gene="rplD"
                     /locus_tag="VFD06_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZN02620.1"
                     /translation="MPRIAVKSLKGGKEKEVTLPDAVYAAPVNTHLVHEAVVAYQAAG
                     RAGTHATKTRSQVSGGGKKPWKQKKTGRARAASIRSPIWKGGGTVFGPQPRDYAHAMP
                     PRKRLGALRSILSAKVRDGRLVLVDSLALEATKSKDLVAAVKGLGVGGSAILVDEPVD
                     RNLALSARNVSHLHATRCTALNAVDLLRFETVVLTEAAAARLAEVLTP"
     gene            complement(9600..10301)
                     /gene="rplC"
                     /locus_tag="VFD06_03435"
     CDS             complement(9600..10301)
                     /gene="rplC"
                     /locus_tag="VFD06_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZN02621.1"
                     /translation="MVEGLIGRKIAMTQVFDDEGRVVPCTVLRVGPCVVVQRREKGRD
                     GYEAVQLGLVEQVKVKKTGKAMKGHFDKANVAPVRTLKEFRLIPGGDDPAVGGKVLVT
                     QFKVADKVDIVGVSKGKGFQGVMKRHHFRGGAASHGSMFHRAPGSVGSSAYPSRTFRG
                     MRMGGRMGGDRVTVKNLAIVRVDEEHNLLVVRGAVPGAGNSLLTVRKARAPIRVVAPQ
                     APTKPLNPLKASKKK"
     gene            complement(10309..10635)
                     /gene="rpsJ"
                     /locus_tag="VFD06_03440"
     CDS             complement(10309..10635)
                     /gene="rpsJ"
                     /locus_tag="VFD06_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZN02622.1"
                     /translation="MLNEKIRISLKAYDHRVLDQSTTEIVATAKRTGARVSGPIPLPT
                     RINKYTVNRSPHVDKKSREQFEVRTHKRLLDILDPTSQTVDALMKLDLPAGVEVEIKA
                     FEKERR"
     gene            complement(10663..11850)
                     /gene="tuf"
                     /locus_tag="VFD06_03445"
     CDS             complement(10663..11850)
                     /gene="tuf"
                     /locus_tag="VFD06_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZN02623.1"
                     /translation="MGKEKFQRTKPHVNVGTIGHVDHGKTTLTSAITMVLNKKFPKIL
                     VRSFDSIDNAPEERERGITIATAHVEYETDNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVSAADGPMPQTREHILLARQVGVPKIVVALNKVDAVDDKELLDLVELEVRELLK
                     SYQFPGDEIPVVKISALKAMQGDAEAQAGILALMEAVDKYIPIPERPIDRPFLMPIED
                     VFSISGRGTVVTGRVEQGKVKVGEEIEIVGIRPTFKRVVTGVEMFKKLLDEGMAGDNV
                     GLLLRGTEKEEVERGQVISKPGSITPHTKYKAEVYILTKEEGGRHTPFFNGYRPQFYF
                     RTTDVTGVATLPQGVEMVMPGDNVSLAVELITPIAMDKGLRFAIREGGRTVGAGTVTE
                     IQA"
     gene            complement(11880..13970)
                     /gene="fusA"
                     /locus_tag="VFD06_03450"
     CDS             complement(11880..13970)
                     /gene="fusA"
                     /locus_tag="VFD06_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZN02624.1"
                     /translation="MARQAPLERTRNIGIMAHIDAGKTTTTERILFYTGITHKLGEVH
                     DGTATMDWMVQEQERGITITSAATTCFWNDHRINIIDTPGHVDFTAEVERSLRVLDGA
                     VGVFCSVGGVEPQTETVWRQADKYKVPRIAFVNKMDRQGADFDRCVRMMKTRLNARPV
                     PIQLPWGREEQFLGIIDLIQMNAIKFVEGTQGAEFEIGPIPEEHKEEVQHARERVIEA
                     ACECSDELLHKYLEGKALKPEEIVAALRKGTIGLHFTPVLCGASFKNKGVQQLLDAII
                     ELLPSPVDKPPIDGTLADGKTEVVRRTSDSEPFAALVFKIMTDPFVGQLAFVRVYSGK
                     LLSGEHVFNATKSQRERIGRLVQMHANKREEIKEVWAGDIAAAVGLRHVRTGDTICDE
                     KSPIVLEAMDFPEPVITLAIEPKTKADQEKLGTALGRLAQEDPTFRISVDPDTAQTII
                     SGMGELHLEIITDRLVREFNVGVNVGKPQVAYKETIRAAAKGEGRYIKQTGGRGQYGH
                     CKIEVSPLPPGSGFEFLNEIVGGSIPKEFIKPIQEGIKEAMLTGPLAGYEMRDLRVTL
                     VDGSYHDVDSSEMAFKIAGSMGFKDATSRARPVLLEPIMSVEVVVPEQYMGDVIGDLN
                     SRRGKVERMEPRAGTQVITAKVPLAAMFGYATDLRSMTQGRANYTMHFSHYDEAPKSV
                     AEGIIARVQGLAKS"
     gene            complement(14059..>14247)
                     /locus_tag="VFD06_03455"
     CDS             complement(14059..>14247)
                     /locus_tag="VFD06_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZN02625.1"
                     /translation="RRTALGIRWIISYSRERSEKSMQEKLANEILDASALRGGAFKKK
                     EDTHKMAEANKAFAHYRW"
BASE COUNT         1891 a         5205 c         4510 g         2641 t
ORIGIN      
        1 gcgaagatga ccgggatgac gccgccggtg ttgagccgca gcggcatgtg ggtctgcatc
       61 ccgccgtaca tccggcggcc gacgacgcgg cgggcgtact gcaccggcac cttgcgctgc
      121 gcccgctcga catagacgat cgccgcgacc accccgacca tcaccgccag caggagcacc
      181 agcatgaacg ggttgagcgt cccggtggag acgtcctgcc acagcgtcgc gatcgccgcc
      241 ggcagcccgg ccacgatgcc gacgtagatg atgagcgaga tgccgttgcc gatgccgcgc
      301 tcggagatct gctcgcccag ccacatgatg aacgccgtgc cggtggtgag cgtcacgatg
      361 gtcatgatgc ggaagaacca gccggggctc gggacgatcg ccccgccgcc gccccccgcc
      421 tggttcaggt tctcgaccca gaaggcgatc ccgagcccct gcaccagcga cagcagcacg
      481 gtgccgtagc gggtgtactg gacgatcttc ttccgcccga cctccccttc cttggacagc
      541 ttctcgaggt agggccagac gaccgtcagg agctgcagga tgatcgaggc ggagatgtac
      601 ggcatgatgc cgagggcgaa gatcgtcagg cgtcggagat tcccgcccga gaaaaggtcc
      661 aggaagccga gcaggccgcc cccctgcgtg cggaagagcg actccagcgc ctcgaagttc
      721 acccccggca cggggatgtg gccgccgacc cggtacaccg ccagcatcag gaaggtgaag
      781 aggacccgct tgcgcaggtc cgggatgctg aagatgttct tgaaggcgtc gatcatgacg
      841 tggcgggttt cgcggccccg atctcctcgg cggtgccgcc ggcggcggtg atcttctcgc
      901 gcgccgagcg gctgaagcgg tgcgcgcgta ccacgagcgc cttggcgagt tcgccgtcgc
      961 cgagcacctt gaggccggcg ccgagcttct tgatcatccc ctccccgacc aggcgctccg
     1021 gggtgatctc accgtcgaga cgggccaggt cgccgacgtt gatgacggcg tactggcgac
     1081 ggaagatgtt ggtgaacccg cgcttcggga tgcggcggtg gagcggcatc tggccgccct
     1141 cgaacccgaa gtactgcgcc gtcgatccgc tgcgcgagag ctgtcccttg ttgccccgcg
     1201 tcgcggtctt tccgtggccc gatccggggc ctcgaccgac gcgcttgcgc ttgcggcgcg
     1261 agcccggcgc ggggcgcagg tgctcgagcc cgaccgtccg gccgtgcggc ctcggcgcct
     1321 ccgccttgtg ggcggccgcc gccttgccgg acggacggcg ggcggccgtg cgcttgccgg
     1381 cccccttgcg ggccgcgccc gccttggggc gggccgtctt gcccttgccg cggtctgcca
     1441 tgtcagctct ccacgatccg gaggaggtgc gggatggtct ccaccatgcc acgcaccgcc
     1501 gggttgtcgg gccgcaccac gcgctgccgg atcttccgga agccgagcga cttcaggacc
     1561 cgcttgtgcg tcacgttgca gccgatgccg ctgcggtact gctcgatggt gaggctcgtc
     1621 ccgcccgccg cgcgccgggc gcgtgccggc ttcttcgcgg ccgtcttctt cttcgcggtc
     1681 tcaggcatcg gtggcgacct ccccggtggt ccccttgcgg cggcgccgct cggcgatcgg
     1741 gtccctcagc atcaagaggc cggcgaacgt cgccttcacg acgttgtgcg ggttggtcgt
     1801 gccgagcgac ttcgtcagca cgtcctggat cccggccgac tccaccaccg cccgcaccgc
     1861 gccgccggcg atgacgccgg tgccgggcgc ggccggcttc aggagcacct gccccgcccc
     1921 gaagatcccc aggacctggt gcgggatggt gtgccccttg agcgggatca agatgaggtt
     1981 gcgcttggcc ttctccaccc ccttggcgat cgcggccggg acctccttgg ccttgccggc
     2041 gccgtagccc acgtggccgc gcccgtcgcc caccacgacc agggcggaga agcggaagtt
     2101 cttccccccc ttgacgacct tggtgacgcg gttgatgtgg accacctggt ccttcagatc
     2161 gagatcgtcc gctttgatcc tgttcacttg atctccctag aacttcagtc cggcggcacg
     2221 cgccgcgtcc gcgaccgcct tgacgcggcc gtgatagagg tacccgccgc gatcgaacac
     2281 caccgtctcg cacccgagcg tcttcaggcg ctccgcgatg agcgtcccca cctgcttcgc
     2341 cgcggcgacg ttcccggtcg gcgtcttacc cttccgcagt tcgagcgtcg aggcgtgagc
     2401 cagcgtgcgt ccctgcgcgt cgtcgatcgc ctggacgtag atgtgaccga ggctgcggta
     2461 gatgcacagg cgcggccgcc cccctgagcc ttcgacgcgg gcgcgcacac gcgcatgacg
     2521 cttctcgcgc gatcggatcc ggtcgacttc gttgaccatg actcgcctac cctcccgccg
     2581 ccgcgccggt cttcccggcc ttccccgcct tcttgcggat cgtttcggtc gagtacttga
     2641 ttcccttccc cttgtacggc tcgggggcgc gcagcccgcg gatctccgcc gccacctggc
     2701 cgaccagctg ccggtccggc cccttcacgg tgaggcgcgt ctgcttgtcg acggcgatgt
     2761 cgatcccatc cgggatcgga tagtccaccg ggtgcgagta gccgagcgcc agctgcagca
     2821 cccttccgtt gacctgcgct ttgtacccga tgccgatgat ctcgaggtcc ttggagaacc
     2881 cgtcgtgcac gcccttgacc gcgttggcga ccagcgcccg catcagcccg tgcagagccc
     2941 gctggcgccg gtcgtcgtcg cggcgggcga gggtcacctt cgtcccttcc acctggcagg
     3001 tgatcccttc cggcagccgg tgggtcaggg aggccttccc tttttcgatg gtcaccgccc
     3061 cgcccgaggt cttgacgttg accccggccg ggagcgtgat cggcatcctt ccgattctcg
     3121 acacgctcgc tccttccccc tcaccagacc gcgcagacga tttcgccgcc gatgccggcc
     3181 ttcttcgccc gcgagccgct catcatcccg cgcggcgtcg acatgatcgt caggccgatc
     3241 ccgcccatga cccgcgggat ggcgtcgtgc cccgcataca cccgcttccc ctgacggctc
     3301 acgcgctcga gcccggagac gaacggccgc ccgtcagcgt cgtagcgcag gcggagcctc
     3361 agcaccttga agcccccctt ttccaccgtc gtgacatctg cgatgtaccc ctcctccttg
     3421 aggatggcgg cgatcgcctc cttgagtcgg gagtggggca tctcggtgcg cgcgtgtcgt
     3481 gcgcggaccg cgttacggat gcgggtcagc aaatcggaaa tcgggtcgtt catccctcgt
     3541 caccctcgcg ccgggcgctc gccgcccggc cctggttcac cagctcgatt tcgtcactcc
     3601 ggggacgagc ccctggagcg acagattgcg gaaacagatg cggcacatct ggaagcggcg
     3661 catgtacgag cgcggccgcc cgcaccgctt gcagcggttg cggtgccgaa tcgcgaactt
     3721 gtgcggcttc ttgaccgccg ccctccacgc cttcgtcgcc atcgtcgttc tcccgtcctc
     3781 agtcgcggcg gaacggcatg ccgagcgcgg cgaggaggtt gcgtgcctcc tcgtcggtct
     3841 tcgccgtcgt cacgatggtc acgttcatgc cgcgcgcctt gtccaccttg gcgtagttga
     3901 tctccaggaa caccagctgg tccttgagac cgaccgtgta gttgccgcgc ccgtcgaacg
     3961 agcggctcgg caggccgcgg aagtcgcgca cgcgcggcag ggtgatcgac accagccggt
     4021 cgaagaactc ccacatccgg tcgccgcgca gcgtcacggt gcagccgatc ggcatgccct
     4081 tgcggagctt gaagttcgcg atcgacttgc gggcgcggcg gatgatcgcc ttctgcccgg
     4141 cgatcgcccc cagctggtcg gcggcggcgt cgagcagctt gatgttctga ctcgcctccc
     4201 ccacccccat gttgatgacg atcttccgca accgcggcac cgccatgacg ttcggatacg
     4261 cgtagcgctc ctgcatctgg cgcgcgatcg tggcgttgta ggcgctcttc agcctcggca
     4321 cgtagccggc cggtgtcgcc gtgggtgcgc tcgtctgggc catctctccg ctccctgtct
     4381 ctcgactact tgtccagcga cccgccgcag cggcggcagg accgcatctt gcggccgtcg
     4441 gcgaggatcg tgcgaccgac ccgcgtcggc ttcttgcact cgccgcacac caccatcagg
     4501 ttgctggcgt ggatcggcgt ctcgcgctcg aggatccccc ccttgatgtt gcgctgcggg
     4561 ttgggtttgg tgtgcttctt gtggaagttc accccctcga cgatcgcccg ccccttggac
     4621 gggtacacgc gcagcacgcg tgcggtccgc cccttgtcct tcccggagag gacaacgacg
     4681 gtgtcgttcc tgcggacgtg cacggcgcgc ggggtcgtgg ccatcacagg acctccggcg
     4741 cgagcgacac gatcttcatg aagttcttct cccgcagctc gcgggctacc gggccgaaga
     4801 tgcgcgtccc gatcggctcg cccgcgtcgt tgatgagcac cgccgcgttg gtgtcgaagc
     4861 ggatgtacgt cccgtcgcgg cgacggtgcg tcttgttcat ccgcacgacc accgccttga
     4921 cgacttgtcc cttcttcacc gtgccgtccg ggatggcctc cttgacgttg gcggtgatga
     4981 cgtcgccgat gccggcgtag aagttgatcg accggccgcg cgtgtggatg cacgagatcc
     5041 tcttggcgcc ggagttgtcg gcgacttcga ggatggtgcg catctggatc atcggtccgc
     5101 tccttgtcct tcctgcattg acacgtcatc gggcggcttc cgccgcgcct gggcgcggcc
     5161 tcggccgcgc gcgttcgtta ccgggccccg gcccgctcca cgatcgactt cacgatccag
     5221 cgcttgtggc gggagagggg tttcgtctcg cggatcgaca cccggtcccc cgcgcggcac
     5281 tcccccttct cgtcgtgcgc catgaagcgc gagcgacgcc ggagatactt ctggtagagc
     5341 gggtgcatca ccagccgctc cacctcgacc acgacggtct tctgcatctt cgcgctcacc
     5401 acgacgccga ccttctcctg ccgccggcgc ggcgcgctct cggtcccggc tgccgcctgc
     5461 tgctgctccg ttgccatcgt ctccccgcgt ctccttgcgt cagcgcgccg gctgctcgcc
     5521 ggcggcccct tccttctcgc gcagcacggt cagcacccgc gccagctcct tgcgcgccag
     5581 cgcgatgcgc tgcgggttct ccgcctgtcc cgtcgccttc tggaagcgga gcttgaagat
     5641 ctgctcccgc agggtcccct cctgctcccg cagctcgttc accgatcgat cccggagggt
     5701 ccgtgccttc acctgggcca tgtcagatcc cccgctcgca gaacatcgtc cggatgccga
     5761 tcttgtgcga cgcgagacgc agcgcctcgc gggcctcctg cgaggtcact ccctccatct
     5821 cgtacaggac gcggcccggc ttgatgacgc agacccagcc ctccggcgga cccttcccct
     5881 tccccatgcg ggtctccgcc ggcttcttgg tgatcggctt gtcggggaag acccgaaccc
     5941 agatctttcc tccgcgcttg atgaagcggg tgatggcgac gcgcgccgcc tcgatctgct
     6001 tgtcggtcat ccagccggcc tccgtcgcct tcagcccata gcggccgaag gcgatcgtgc
     6061 cgccgcgcca ggccttgccg cggcgccgcc cgcgctgctg cttccggtac ttgaccttct
     6121 tgggcatcag catgctgcgt cgtctcttct ctgatcgcgc ggcttccgcc gcgctttcgt
     6181 ctcatcgcgc ggcctgagcc gcgctttcgt ctagatttgc gccaggccga ggcggtcctg
     6241 cggccgcgtc agccccttga gctcgccctt gtagatccag cacttcacgc cgatctgccc
     6301 gtaggtggtg cgggcctcgg cgaagccgaa gtcgatgtca gcccgcagcg tgtgcagcgg
     6361 cagctggccg tcgaggtacc actccgagcg cgcgatctcc gatccgccga gccgccccga
     6421 acagcgcagc ttcacgccct tggccccgaa gcggaccgcc gactcgatcg ccttcttcat
     6481 ggcgcgccgg aacgcgacgc ggcgttcgat ctgcaggccg accgactccg ccaccagctg
     6541 cgcctcgagc tccggcttgt ggatctccat gatgttgatg aagatctcgc ggccggtccg
     6601 cttctgcaga tcgtcgcgca gcttgtccac ctccgccccc ttgcggccga tgatgatccc
     6661 cgggcgcgag gtgtggatgg tgatcttcag cttgttggcg gcgcgctcga tgtcgacgcg
     6721 ggccatgccg gcgtgggaca gccgcttctt caggatgccg cgcaggtcga gatcctcgtg
     6781 cagcatgctg gcgtagttct tctcggcgta ccaccgcgac tgccaggtgc ggttgtaccc
     6841 gagccggaaa ccgaacggat ggaccttctg acccaaggct gctcctttac gggcgcgcgg
     6901 cgttagccgc cgcccccttt cttgcttccc gaaccgcgca tgcgggcctt ccccttcgcc
     6961 gggccgcccg ccggcccttc cttgcgcgcc ggccgcgtcg cggcgtggcg cttgtgcgtc
     7021 gtccgcgacc caccggccgg cgccgccgaa gcggcgtgct ccttcgcgga cggctcggcc
     7081 ccctcggcct tcgccgacgg ccgccgcgcc gcggccgcgg gacgaccgtc gccggtctcc
     7141 acgagcccga gcgtcacgtg gcacatccgc ttctgcaccc ggaaggcccg ccccatcggg
     7201 gccggccgga tgcgcttcag catcggaccg ccgtcgacga acgccgtacc cacgacgagc
     7261 cgatccacgt ccacctccgg ccgcttcacc cgcgcgttgg cgaccgcgga gtcgagcgcc
     7321 ttgaggatct cccgcgccac cgccttgggc gtgaagcgca gcgtcccgag cgcctcctcg
     7381 acccggcggc cgcggatgag atccacgacc aggcgcgcct tctgcgccga ggttccgagg
     7441 taacggagcc gaacgtggcc gacgagtccc gctggcagca tcgcgtctct ccgcctattt
     7501 cggtgtggcg accacggccg ccggaggacc gcccggcccg cccggtcccg cccctgccgg
     7561 ggcggccgcg gccgagtcgg cgcgcgagtg accgcggaag gtgcgcgtgg cggcgaactc
     7621 gccgagcttg tgcccgacca tgttctccga cacgtacacc gggatgaact tcttgccgtt
     7681 gtggacggcg atcgtgtgcc cgaccatctc cggcacgatg gtgctccgcc gcgaccaggt
     7741 cttcatgacc cgcttctcgc tgcggcggtt catctcctcg atgcggcgcc tcagcaccgg
     7801 gtcgacgaag gcccctttct tgatgctccg tgccatgggc tctccctact tcctgcgccg
     7861 aacgatgtat ttgtcggtcc gcttgttgtt gcgcgtcttg cgctcgcgca cgccccatgg
     7921 cgtgcacggg tggcgcccgc cggaggtctt cccctcgccc cctccgagcg ggtggtcgat
     7981 cgggttcatg gcgacgccgc gcaccgtcgg ccggatgccg agccagcgcc ggcgccccgc
     8041 cttaccgatg gagacgttct cgtggtcgag gttgccgacc tgtccgatgg tcgcctggca
     8101 cgtctccagg accttgcgga tctcgcccga cggcagcttc acctgcgccc agccggcgtc
     8161 gcgcgccatc agctgcgcga cgctgccggc actgcgtacc atctgcccgc cgcgccccgg
     8221 cttcagctcg atgttgtgga tcccggtgcc gagcgggatc gccttgagcg gcaggacgtt
     8281 gcccggcagg atgtcggcct cgggccccgc cagcaccgtc tgcccgacgt gcaggcccag
     8341 cggcgccagg atgtagcgct tgtcgccgtc ggaatagtgc acgagggcga tgcgggccga
     8401 gcggttgggg tcgtattcga tggtcgcgac cttccccggg atccccgtct tgtcgcggcg
     8461 gaagtcgatg gtgcgcagca ggcggcgatg cccgcccccc ctgtggcgca ccgcgatgcg
     8521 cccgatgttg ttccgcccgc cggtgcggcg cttgccgacg gcaaggcgtt tgagcgggcg
     8581 gacgtcgctc agatcgtcga aaacgatcgt cgagtagtag cggcgcgcgg gagacgtggg
     8641 acggctgttc ttgatcgcca tggcggttcc tacacgacct cgaagtattc gatcgacttc
     8701 tggcccggct tgagcgtgac gtacgccttc ttccagtccg ggcgcttccc ctcgaagcgc
     8761 ccgacgcgct tcaccttgcc gtgcacgttc tcggtgcgga cggaggccac cttcaccttg
     8821 aagagcgcct ccaccgcctt cttcacctcg atcttgttcg cgcggcgctc gaccttgaag
     8881 gcgaggacgt tgcgcccctc cttcaggatc tgcgtcttct cggtgatcag cggtccgatc
     8941 agcacgtcct ggggccggtt catggcgtca gcacctcagc gaggcgcgcg gcggccgcct
     9001 cggtcagcac gaccgtctcg aagcgcagca ggtccacggc gttcagcgcg gtgcagcggg
     9061 tcgcgtgcag gtgcgagacg ttgcgcgccg agagcgcgag gttccgatcc accggctcgt
     9121 ccaccaggat ggcggatccg ccgacgccga gtcccttcac cgcggcgacc aggtccttgc
     9181 tcttcgtcgc ctcgagggcg agcgagtcga cgagcaccag gcgtccatcc cgcactttgg
     9241 cggagagaat ggaacgcagc gcccccaggc gcttgcgcgg cggcatggcg tgggcgtagt
     9301 cgcgcggctg cgggccgaac acggtgccac cccccttcca gatcggagag cggatcgagg
     9361 cggcgcgggc gcgcccggtc ttcttctgct tccagggttt cttgccgccg ccactcacct
     9421 gcgagcgggt cttggtggcg tgcgtgccgg cgcggccggc cgcctggtac gccaccaccg
     9481 cctcgtgcac gaggtgggta ttgaccggcg ccgcgtagac ggcgtcgggg agggtgacct
     9541 ccttctcctt gccgcccttc aggctcttca ccgcgatgcg tggcatcagt acgctcccct
     9601 cacttcttct tcgaggcttt cagcggattg agcggcttgg tcggcgcctg cggcgcgacc
     9661 acccggatcg gcgcgcgcgc cttgcgcacc gtgagaaggg agttccccgc ccccggcacg
     9721 gcgccccgca cgaccagcag gttgtgctcc tcgtccaccc ggacgatcgc cagattcttc
     9781 acggtcaccc ggtcgccgcc catccggccg cccatgcgca tgccccggaa ggtgcgcgac
     9841 gggtaggccg acgaaccgac cgagccgggg gcgcgatgga acatcgagcc gtggctggcc
     9901 gcgccgccgc ggaagtggtg ccgcttcatg acgccctgga accccttccc cttcgagacg
     9961 ccgacgatgt cgaccttgtc ggcgaccttg aactgtgtca ccaggacctt gccgccgacc
    10021 gccggatcgt ccccgccggg gatgagccgg aactccttca gggtccgcac cggcgcgacg
    10081 ttggccttgt cgaaatgccc cttcatcgcc ttgccggtct tcttgacctt gacctgctcg
    10141 acgaggccca gttgcaccgc ctcgtagccg tcgcgcccct tctcgcggcg ctgcacgacg
    10201 acgcacggcc cgacccgcag caccgtgcag gggaccacgc ggccttcgtc gtcgaagacc
    10261 tgggtcatgg cgattttccg gccgatcagt ccttcgacca tctcgtcctc agcgccgttc
    10321 cttctcgaac gccttgatct cgacctcgac ccccgccggc aggtcgagct tcatgagcgc
    10381 gtcgaccgtc tgcgaagtcg gatcgaggat gtccaggagc cgcttgtgcg tccgcacctc
    10441 gaactgctcg cgcgacttct tgtccacgtg cggcgagcgg ttcacggtgt acttgttgat
    10501 gcgggtcggg agcgggatcg gcccagacac ccgggccccc gtgcgcttgg ccgtggcgac
    10561 gatctcggtc gtcgactggt cgaggacccg gtgatcgtac gccttcaggc tgatgcgaat
    10621 cttctcgttg agcatatccc ccgatccttc gtctctctcg ccttacgcct ggatctcggt
    10681 gacggtgccg gcgccgacgg tgcggccgcc ctcgcggatg gcgaagcgca gccccttgtc
    10741 catcgcgatc ggcgtgatca actccaccgc cagactcacg ttgtcccccg gcatcaccat
    10801 ctccacaccc tgcggcagcg tcgccacgcc cgtcacgtcc gtcgtccgga agtagaactg
    10861 cggccggtat ccgttgaaga acggcgtgtg ccgtcccccc tcctccttcg tcaggatgta
    10921 cacctccgcc ttgtacttcg tgtgcggcgt gatgctccca ggcttcgaga tcacctgacc
    10981 ccgctccact tcctctttct ccgtcccgcg caaaagcagc ccgacgttgt ctcctgccat
    11041 cccctcgtcg agcagcttct tgaacatctc cacgcccgtc accacgcgct tgaacgtcgg
    11101 ccggatcccg acgatctcga tctcctcccc gaccttcacc ttcccctgct ccacccgtcc
    11161 cgtcaccacc gtcccgcgcc ccgaaatcga gaacacgtcc tcgatcggca tcaggaacgg
    11221 ccgatcgatc gggcgctccg gaatcgggat gtacttgtcc accgcttcca tcagcgccag
    11281 gatccccgcc tgcgcttccg cgtccccctg catcgccttc agcgccgaga tcttcaccac
    11341 cggaatctcg tccccgggga actggtagct cttcagaagc tcacgcacct ccagctccac
    11401 cagatccaaa agctccttgt cgtcgacggc gtccaccttg ttcaacgcca ccacgatctt
    11461 cggcaccccc acctgccgcg ccagaagaat gtgctcccgc gtctgcggca tcggcccgtc
    11521 cgccgccgac accaccagga tcgccccgtc catctgcgcc gctcccgtga tcatgttctt
    11581 cacgtagtcg gcgtgccccg gacagtccac gtgcgcgtag tgacgattgt ccgtctcgta
    11641 ctccacgtgc gccgtcgcaa tcgtgatccc gcgctcccgc tcctccggcg cgttgtcgat
    11701 cgagtcgaac gaccgcacca gaatcttcgg gaacttcttg ttcaacacca tcgtgatcgc
    11761 cgacgtcaaa gtcgtcttcc cgtgatccac gtgtccgatc gtccccacgt tcacgtgcgg
    11821 cttcgtccgc tggaatttct ccttgcccat cctcgatcct ccgcgctctt cggacgcgac
    11881 taggacttcg cgagcccctg gacccgcgcg atgatgccct cggcgacgct cttcggtgcc
    11941 tcgtcgtagt gcgagaaatg cattgtgtag ttcgcccgtc cctgcgtcat gctgcgcagg
    12001 tcggtggcgt agccgaacat cgccgccagc ggcaccttgg cggtgatcac ctgggtgccg
    12061 gcgcgcggct ccatgcgctc caccttgccg cgccgcgagt tcaggtcgcc gatcacgtcc
    12121 cccatgtact gttccggcac caccacctcg acgctcatga tcggctcgag gagcaccggg
    12181 cgggcgcgcg acgtcgcatc cttgaatccc atggagccgg cgatcttgaa cgccatctcc
    12241 gacgagtcga cgtcgtgata cgagccgtcg acgagcgtca cgcgcaggtc gcgcatctcg
    12301 tagccggcga gcgggcccgt cagcatggcc tccttgatcc cctcctggat cggcttgatg
    12361 aactccttgg ggatcgagcc gccgacgatc tcgttgagga actcgaagcc gctgccgggc
    12421 gggagcggcg acacctcgat cttgcagtgg ccatactggc cgcggccgcc ggtctgcttg
    12481 atgtacctcc cctccccctt ggcggcggcg cggatcgtct ccttgtacgc gacctggggc
    12541 ttgccgacgt tgacgccgac gttgaactcg cgcaccaggc gatcggtgat gatctcgagg
    12601 tgcagctccc ccatcccgga gatgatcgtc tgggcggtgt ccgggtcgac gctgatgcgg
    12661 aaggtggggt cctcctgcgc caggcggccg agagcggttc ccagcttctc ctggtcggcc
    12721 ttggtcttcg gctcgatggc gagcgtgatg actggctccg ggaagtccat cgcttcgagc
    12781 acgatcgggg acttctcgtc acagatggtg tccccggtgc gcacgtgacg cagcccgacg
    12841 gcggcggcga tgtcgccggc ccagacctcc ttgatctcct cgcgcttgtt ggcgtgcatc
    12901 tgcaccagcc ggccgatgcg ctcgcgctgc gacttggtgg cgttgaagac gtgctcgccc
    12961 gacagcagct tgccggagta gacgcgcacg aacgccagct gacccacgaa cgggtcggtc
    13021 atgatcttga agacgagcgc cgcgaacggc tcgctgtcgg aggttcgccg caccacctcg
    13081 gtcttgccgt ccgccagggt gccgtcgatc ggcggcttgt cgaccggcga gggcaggagc
    13141 tcgatgatgg cgtcgaggag ctgctgcacg cccttgttct tgaacgacgc gccgcacagc
    13201 accggggtga agtgcagccc gatcgtccct ttgcgcaggg ccgcgacgat ctcctccggc
    13261 ttgagcgcct tcccctcgag gtacttgtgc agcagctcgt cggagcactc acacgccgcc
    13321 tcgatgaccc gctcgcgcgc gtgctggacc tcttccttgt gctcctcggg gatcgggccg
    13381 atctcgaact cggccccctg cgtcccctcg acgaacttga tcgcgttcat ctggatgagg
    13441 tcgatgatcc cgaggaactg ctcctcgcgc ccccacggca gctggatcgg gaccgggcgc
    13501 gcgttcagcc gcgtcttcat catgcggacg cagcggtcga aatcggcccc ctggcggtcc
    13561 atcttgttga cgaaggcgat gcgcgggacc ttgtacttgt ccgcctggcg ccagaccgtc
    13621 tcggtctggg gctccacccc gccgaccgag cagaagacgc caaccgcacc gtccaggacg
    13681 cgcagcgagc gctccacctc ggcggtgaag tcgacgtggc ccggggtgtc gatgatgttg
    13741 atgcggtggt cgttccagaa gcaggtcgtg gcggccgagg tgatcgtgat cccgcgctct
    13801 tgctcctgga ccatccagtc catggtcgcg gtgccgtcgt gcacctcgcc gagcttgtgg
    13861 gtaatccccg tatagaagag gatgcgctca gtcgtcgtcg tcttaccggc atcgatgtgg
    13921 gccatgatgc cgatattgcg agtcctctcc agaggtgcct ggcgcgccac gtcaaagatc
    13981 catgcgcccc cggtgtgacc cgggtcgctt tgtgactgtc gactgggaac cgttaactgt
    14041 taacggttca cagctcagct accaccgata gtgcgcgaag gccttgttgg cctccgccat
    14101 cttgtgcgtg tcttccttct tcttgaacgc gccgccgcgg agggcggaag cgtccaagat
    14161 ctcgttcgcg agcttctctt gcatcgactt ttcggaccgc tcccgcgagt aggagatgat
    14221 ccagcgaatc cccagcgccg tgcgtcg
//
LOCUS       DASRIQ010000140         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1620298, whole genome shotgun sequence.
ACCESSION   DASRIQ010000140 DASRIQ010000000
VERSION     DASRIQ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620298"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFD06_03460"
     CDS             <1..237
                     /locus_tag="VFD06_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase"
                     /protein_id="HZN02626.1"
                     /translation="RSAGCEQQAFALGARVAGLQFHLEFSGDTVQDLLRFCPRDLAPG
                     PYVQTPEAMLWEPARFDAGHAVLESVLDAMSGGV"
     gene            276..>2870
                     /locus_tag="VFD06_03465"
     CDS             276..>2870
                     /locus_tag="VFD06_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HZN02627.1"
                     /translation="MKAVDALFGGAPPEPEPIRAEVFGIERLEQHAESLAAAHRTPGT
                     PPRGRPLLSRVRDNAHVLLAGYRSIAETVMAKQEITPAAEWILDNFHVVDEQLRGIRD
                     HLPRGYDRRLPKIATGHLAGDSRVYGLAWAYVAHTDSRFEMETIQRFVRAYQRVQPLT
                     IGELWAVAIHLRVALVENLRRLSQAIIRSRRERARADALADRLLGLGARPEGSPSEVL
                     ADLGDAPLTRAFAVQLVQRLRGQDPFIMPALAWLDTKMSAQGTSADEAVAQEHQAQAA
                     DNVTVRNIITSMRWMSSIDWTVFFESVSLVDEVLRTAPGFAAMDFPTRDRYRARIELL
                     SRGSSRSEMDVAREAVRVARDAGREPGPRTLADVPERAEEDPGHSILAGGRRAFERRL
                     GFRVPLRIRLRRAYRAHPIAGYLGAIVALTALLLSGPLVLTAAAGAAPWVLVLLGLLG
                     LVPASDLAVSLVQRLVPLLIPPRLVPKLALAGGVPPELRTLVVVPTLLRRRTDIEEQL
                     ERLEVHYLANPEGEIHFALLTDWADATTEHVPGDEDLVAELAAGIARLNRRYGIAPSG
                     GPRFLLLHRRRLWNGQERRWIAWERKRGKLHELNRLLRGATDTTFIPIAGRPPAVPQG
                     VRYVITLDADTRLPMGTARRLVGAMAHPLNRPRFDSRKGRVVDGYAILQPRLTPCLPT
                     GPDSTTYQRIVSGPGGVDPYAAAISDVYQDLFGEGSYTGKGIYDVDAFEAALEGKVPE
                     NTLLSHDLFESAFARTGLATDVDLFEEFPSNYEVAAQRQHRWVRGDWQLLPWILGRRM
                     RIPGLGRLKMVDNLRRSLAAPAAFLLAVAAWTLPNVPPLLWTALFVGSMVLPPVIPVL
                     DGLLPRRLG"
BASE COUNT          390 a         1049 c          994 g          437 t
ORIGIN      
        1 cgcagcgccg gctgcgagca gcaggcgttc gccctcgggg cacgggtcgc cggactgcag
       61 ttccacctcg agttcagcgg ggacacggtg caggatctct tgaggttctg cccgcgcgac
      121 ctcgcgccgg gtccctacgt ccagacgccg gaggcgatgc tgtgggagcc ggcccgcttc
      181 gacgccggtc acgcggtgct cgagagcgtg ctcgacgcga tgtccggcgg cgtgtgagcc
      241 ggcccgtcgc cgcatccttc cgcgggttcc gcgcagtgaa ggccgtcgac gccctcttcg
      301 gcggcgcgcc tccggagccg gagccgatcc gcgccgaggt gttcgggatc gagcgtctgg
      361 agcagcatgc cgagagcctg gccgcggccc atcgcacgcc cgggacgccg ccgcggggac
      421 gcccgctcct ctcccgggtc cgggacaatg cgcacgtcct cctcgccggg taccggagca
      481 tcgccgagac cgtcatggcg aagcaggaga tcacgccggc ggcggaatgg atcctcgaca
      541 acttccacgt cgtggacgag cagctgcggg ggatccggga ccacctgccg cgagggtacg
      601 accgacggct gccgaagatc gccaccggcc acctcgcggg agactcacgc gtctacgggc
      661 tggcctgggc ctacgtcgcc cacaccgaca gccggttcga gatggagacg atccagcggt
      721 tcgtccgcgc ctaccagcgg gtccagccgt tgaccatcgg ggagttgtgg gcggtcgcca
      781 tccatctccg cgtcgccctg gtggagaacc tgcgacgcct gtcgcaggcg atcatccgct
      841 cccgccggga gcgggcccgg gccgacgcgc tggcggatcg cctgctgggc ctgggggctc
      901 gtccggaggg gagtccgagc gaggtcctgg ccgatctggg agacgctccg ctgacccgcg
      961 ccttcgccgt gcagctcgtc cagcggctgc gcggccagga tccgttcatc atgccggccc
     1021 tggcgtggct cgacacgaag atgagcgccc aggggacctc ggccgacgaa gcggtcgcgc
     1081 aggaacacca ggcccaggcc gcggacaacg tcaccgtgag gaacatcatc accagcatgc
     1141 gctggatgtc ctccatcgac tggaccgtgt tcttcgagag cgtcagcctg gtggacgaag
     1201 tcctccgcac cgctcccggc ttcgcggcca tggacttccc gacccgggac cgataccggg
     1261 cgcgcatcga gctcctgtcg cgcggctcgt cacgatcgga gatggacgtg gctcgggagg
     1321 cggtgcgcgt cgcccgcgac gccggccggg agcccgggcc gcggacgctc gcggacgttc
     1381 cggagcgcgc cgaggaggac cccggtcatt ccattctcgc cggggggcgg agagcgttcg
     1441 agcgacgcct ggggtttcgc gtccccctgc gcatccgtct gcgccgcgct tatcgcgccc
     1501 acccgatcgc cggttatctg ggagccatcg tcgcgctcac cgccctcctg ctgtccggcc
     1561 cattggtcct gaccgcggcg gcgggagcgg ccccctgggt cctggtcctc ctggggcttc
     1621 tcggcctggt gccggcctcg gatctcgcgg tgtcgctcgt ccagcgcctc gtcccgctgc
     1681 tcattccgcc ccggctggtc cccaagctcg cgctcgccgg gggcgtcccc ccggagctgc
     1741 gcacgctcgt cgtcgtcccc acgctgctgc ggcgccggac cgatatcgaa gaacagctcg
     1801 agaggctcga ggtccactac ctggcgaatc ccgaagggga gatccacttc gccctgctca
     1861 ccgactgggc cgacgcgacc accgagcacg tccccggcga cgaggacctc gtcgccgagc
     1921 tggccgccgg catcgcccgc ctcaatcggc gctacgggat cgctccgagc ggcggaccgc
     1981 gattcctgct gctgcaccgc cggcgcctgt ggaacggcca ggaacggcgg tggatcgcct
     2041 gggagagaaa gcggggcaag ctgcacgagc tgaaccggct gctgcgcggc gccacggaca
     2101 ccaccttcat cccgatcgcc ggccgtccgc cggccgtgcc gcagggagtt cgctacgtca
     2161 tcaccctgga cgccgacacc cggctcccca tgggaaccgc ccgtcgactg gtcggggcga
     2221 tggcgcatcc tttgaaccgg ccgcgtttcg actcccggaa gggacgggtc gtcgatggct
     2281 atgcgatcct ccagccgcga ctcaccccct gcctgccgac ggggccggac agcacgacct
     2341 accagcggat cgtctcgggt cccgggggtg tggatcccta tgcggccgcg atctccgacg
     2401 tctatcagga cctgttcggg gaagggtcgt acaccggaaa ggggatctac gacgtcgacg
     2461 ccttcgaagc ggcgctcgag ggaaaggtcc cggagaacac cctgctcagt cacgatctgt
     2521 tcgaaagcgc cttcgcgcgc accggcctgg cgaccgacgt ggatttgttc gaggaattcc
     2581 cctccaacta cgaggtggcg gcgcaacgcc agcatcgatg ggtccggggg gactggcagc
     2641 tgctcccgtg gatcctgggc cggaggatgc ggatccccgg tcttggccgc ctcaagatgg
     2701 tggacaatct ccgccggagt cttgcggcac ccgcggcatt cctgctcgcg gtggccgcct
     2761 ggaccctccc caacgtcccc ccgctcctct ggaccgccct gttcgtcggg tcgatggtcc
     2821 tgccgccggt gatcccggtg ctcgacgggc tgctcccgcg gcgcctgggg
//
LOCUS       DASRIQ010000141         4917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2931829, whole genome shotgun sequence.
ACCESSION   DASRIQ010000141 DASRIQ010000000
VERSION     DASRIQ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2931829"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2199
                     /locus_tag="VFD06_03470"
     CDS             <1..2199
                     /locus_tag="VFD06_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02628.1"
                     /translation="DVTFRLYDAAAGGTELLVDRHLAANGQAVVVGGGLFSTQLGGGA
                     VSDGSGAGTYASLADVFRAHASVWVGIEIGAETLSPRLRVVASAYALNADAVDGKNGA
                     DLIDTSESFQLKEGRLAVGGSTGDWYGVEGFGFDGGGYFSNVGGNSALIAAPGGVGAW
                     ALGTNMGGYFKNTTTLSSPSVVLADATNGVIVTGAAVTGIDSQGSGAAGYFHETDSAA
                     SAYVASLGYGIQASGPAAGGYFFDATGSGYAYLGLNDIGVAGYGSQYGGYFRDTDMSA
                     SATLAVVGYGVSASGSAGGAQFLDTGGTSNAYIAHGDLGVEAAGNGAGAYLGDKNNSG
                     FSYVGFGDEGIYSAGLLDGGFFDDLDTSSFARVGYSTYKIFGSGSVSFAQNHPYDKDK
                     VIVYVAPEGDEAAVYTRGTARLAGGRARVALGETFALVANPDVGLTAHLTPRGKSADL
                     FVESLGTGEMIVRGPDGSDAEFDYMVWGLRLGFEEQSIVQPKQLESMIPSMADHQAFY
                     ASDPSLRDYNALARHTTMERAARGGAAIDLSRSRRMIEAIGVYEPAVDGEVQALHARP
                     RRPEPGARPVPDPDAMSEAAAGMLPAAETAATPVGPVIGLPVIPYEPLAADGPVSPGD
                     VVVLDPASAGSIRRSDIAGDTHVIGCVVEARDPSALRQGEAAVAVSRIATCRAEATER
                     PITSGDLLVVSTIPGHAMAAASPAPGQILGKALDPLQGGTGLIRVLIGAR"
     gene            2211..2786
                     /locus_tag="VFD06_03475"
     CDS             2211..2786
                     /locus_tag="VFD06_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02629.1"
                     /translation="MRSRLEERAFPAVVPLVLAVLAIGASASTATAQSSPGYRIDGSV
                     FNLGGRPESGHVAASPAFKVTLDSLGEPATATLTSGLFQATGGLPAAWPPPGEVLGLA
                     FDSPTHLFWNPERSAGAYQVYRAPSGTFPASYGSCLVGHLGVTEKDEVEVPPVGAAFL
                     YHVTVSNRLGEEGTLGRTSAGALRPNLSPCP"
     gene            2777..3403
                     /locus_tag="VFD06_03480"
     CDS             2777..3403
                     /locus_tag="VFD06_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02630.1"
                     /translation="MPLTGVPRRSLAARAGFVTLLLGLLAGSAPAHAASPRPLGRWHG
                     WLDVGRVDLDEEATQDWGVDREGYLALSVSHGDDGRWYWGVELGHAKKGEATDGETGL
                     IRDFDFGWIDLDQKWAFDLGHGWSADAGLGLAVFYVDGERETILGSGEVFFSSLAGFG
                     LGAQIVVDASWRTGHLLLGAGVRYQWAYDIIDINHSNLRFGAHLGLVF"
     gene            complement(3405..4778)
                     /locus_tag="VFD06_03485"
     CDS             complement(3405..4778)
                     /locus_tag="VFD06_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02631.1"
                     /translation="MTRRLTLDRLVLALALCAGLPGTIGALFLLWLGEFALKTQITFS
                     LLVILPWMMFAFAARERVLRPLQSVANLLAALGEGDFSLRGRGRGPDDPLEDVMREIN
                     VLGDTLREQRLGALEAGALLRKVIDEIDVGIFAFDDEGRLRLANRAAERLIGRPAEQL
                     LGRDIASLGLYACFTEGGGVPQGVAGARILDLAFAGGSGRYEARTGTFRQGGVPHRLL
                     VLADVSRALRDEERQAWQRLIRVVGHEMNNSLAPIRSIADSLRTLLHAERLPSDWRED
                     ARGGLSIIATRAESLGRFMDAYARLARLPPPRLAPVAIGTIVRRVAGLETRTSIAVRP
                     GPDLTIEADADQLEQLLINLTRNAVDAAQETGGGVTLGWRAMRGGPSPRVEIAVEDGG
                     PGLPQSANLFVPFFTTKPQGSGIGLVLCRQIAEAHGGSLTLENRKDAPGCVARLTLPL
                     PRRQRAA"
     gene            complement(4775..>4917)
                     /locus_tag="VFD06_03490"
     CDS             complement(4775..>4917)
                     /locus_tag="VFD06_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN02632.1"
                     /translation="DLPLEDVERLLIRKALERHGGNVSQAATALGLSRSALYRRLQRH
                     GL"
BASE COUNT          685 a         1714 c         1780 g          738 t
ORIGIN      
        1 gacgtcacct tccgactcta tgacgccgcg gccggcggca cggagcttct cgtggatcgc
       61 catctcgcgg cgaacgggca ggcggtggtc gtcggcggcg ggctcttctc gacgcagctc
      121 ggcggcggcg ccgtctccga cggttcggga gccggcacct acgcgtcgct cgcggacgtg
      181 ttccgggctc acgcctccgt ctgggttggc atcgagatcg gcgccgagac gctctccccg
      241 cggctgcgtg tcgtcgcgtc ggcgtatgcc ctgaacgccg acgcggtcga cgggaagaac
      301 ggcgccgacc tcatcgacac cagcgagtcg ttccagctca aggagggacg cctcgcggtg
      361 ggaggatcga ccggggattg gtacggggtc gagggattcg ggttcgacgg cggcggctat
      421 ttctccaatg tgggcggcaa cagtgcgttg atcgccgctc cgggcggtgt cggggcgtgg
      481 gcgctcggca ccaacatggg cggctatttc aagaacacga ccacactgtc gtccccatcc
      541 gtggtgctgg ccgatgcgac gaatggcgtc attgtcacgg gggctgcggt cacggggatc
      601 gacagtcagg gaagcggggc cgccggctat ttccacgaga ccgacagcgc cgcctccgcc
      661 tacgtggcca gcctggggta cgggattcaa gcctcgggac ccgctgccgg cggctacttc
      721 ttcgacgcga ccggcagcgg ctacgcctac ctcggcttga acgacatcgg tgtcgccgga
      781 tacggcagtc agtacggcgg ctacttccgg gacaccgaca tgtcggcgtc ggccaccctg
      841 gcggtggtcg gctatggcgt gtccgcgtcc gggtctgccg gcggcgcgca gttcctggac
      901 accggcggca cgagcaacgc ctacatcgcg cacggcgatc tcggtgtgga agcggcgggt
      961 aacggcgcgg gcgcctacct cggcgacaag aacaactcgg gcttctccta cgtcgggttc
     1021 ggcgacgaag ggatctactc ggctggcctg ctcgacggcg ggttcttcga cgacctcgat
     1081 acttcgtcct tcgcccgggt cggctactcg acctacaaga ttttcggcag cggctcggtc
     1141 tcctttgcgc agaaccaccc ctacgacaag gacaaggtga tcgtctacgt ggcgccggag
     1201 ggggacgagg cggcggtcta cacccgcggc acggcgcgcc tcgccggcgg ccgggcacgc
     1261 gtcgcgctcg gcgagacctt cgcgctcgtc gccaaccccg acgtcggcct gacggcccac
     1321 ctgaccccgc gcggcaaatc ggccgacctg ttcgtggaga gcctcggcac gggcgagatg
     1381 atcgtgcggg ggccggacgg ctccgacgcc gagttcgact acatggtctg ggggctgcgg
     1441 ctcgggttcg aggagcagag catcgtccag ccgaagcagc tcgagtcgat gatcccgtcc
     1501 atggcggatc accaggcgtt ctacgcgagc gacccgtctc tgcgcgacta caacgctctg
     1561 gcgcgccaca cgacgatgga gcgcgccgcc aggggcgggg cggcgatcga tctgtcgcgc
     1621 tcgcggcgga tgatcgaggc gatcggcgtc tatgaaccgg cggtcgacgg cgaagtccag
     1681 gcgctgcatg cgcgtccccg ccggccggag ccgggagcca ggcccgttcc ggacccggat
     1741 gcgatgtcgg aagctgcggc aggcatgttg ccggccgccg aaaccgccgc gactccggtc
     1801 ggcccggtga tcgggctgcc ggtgattccc tacgaacccc tcgctgccga cggtccggtg
     1861 agtcccggcg acgtggtcgt cctcgatccc gcctcggccg gatccatccg tcgctcggac
     1921 atcgcgggcg acacgcacgt catcggctgc gtcgtggagg cccgggatcc ctccgccctg
     1981 cgccagggcg aagcggccgt cgccgtgtcg cggatcgcca cctgccgcgc cgaagcgacc
     2041 gagcggccga tcacgagcgg cgatctgctc gtcgtctcca ccattccggg gcacgcgatg
     2101 gccgccgcct cgccggcgcc gggccagatc ctcggcaagg ccctcgaccc tctccagggc
     2161 ggcaccgggc tcatccgcgt cctcatcggg gcgcggtgag gaggtgaatg atgcgatcga
     2221 gactggaaga gcgcgcattt cctgccgtcg ttcccctggt gctggccgtt ctggcgatcg
     2281 gcgcttcggc ctcgacggcg acggcccaga gcagcccggg ctaccgcatc gacggatccg
     2341 tgttcaacct gggcggccgg ccggaatccg gccatgtcgc agcctcaccc gccttcaagg
     2401 tcacgctcga ctccctgggg gaaccagcga ccgccacgct gaccagcggc ttgttccagg
     2461 cgacgggcgg ccttccggcc gcatggccgc cgccggggga ggtgctcggc ctggcgttcg
     2521 actctccgac acacctgttc tggaatcccg agcgttcggc cggcgcctac caggtttacc
     2581 gcgcgccctc gggaacgttc ccggcctcct acggttcatg ccttgtggga cacctcgggg
     2641 tgacggagaa ggacgaggtc gaggtgcctc cggtcggtgc ggcgtttctc tatcacgtga
     2701 cggtgtcgaa ccggctcggc gaggagggga ccctcggccg gaccagcgcc ggcgccctgc
     2761 ggccgaacct cagtccgtgc ccctgaccgg cgtgccgcgc cggtccctcg ccgccagggc
     2821 aggcttcgtg accctcctgc tcggccttct cgccggctcg gcgccggcgc acgccgcctc
     2881 gccgcgcccg ctcgggcgct ggcacgggtg gctggacgtc gggcgcgtcg atctcgatga
     2941 ggaggcgacg caagactggg gggtggatcg cgaggggtac ctcgccctct ccgtgtccca
     3001 cggcgacgac ggcaggtggt actggggcgt ggagctgggt cacgcgaaaa agggggaggc
     3061 caccgacggc gagaccggcc tgatccggga cttcgatttc ggctggatcg atctcgacca
     3121 gaagtgggcc ttcgacctcg gacacggctg gtcggccgac gccggactgg gcctggccgt
     3181 gttctacgtc gatggcgaaa gagagacgat ccttgggagt ggtgaggtgt tcttcagctc
     3241 gctcgccggt ttcggcctcg gcgcgcagat cgtcgtggat gcctcctgga ggaccggaca
     3301 cctcctgctg ggcgccggtg tgcgctacca gtgggcctac gacatcatcg acatcaacca
     3361 ttccaacctg agattcggcg cccacctcgg gttggtcttc tgactcaggc cgcgcgctgc
     3421 cgcctcggga gcggcagggt gagccgcgcc acgcaccccg gcgcgtcctt gcggttctcg
     3481 agggtgagcg agccgccgtg cgcctcggcg atctgccggc acaggacgag cccgattccg
     3541 gatccctgcg gcttggtggt gaagaacggc acgaagagat tggcgctctg cggcaggccg
     3601 gggccgccgt cctcgacggc gatctcgaca cgcggggagg ggccgccgcg catcgcgcgc
     3661 cagccgaggg tgacgccgcc tccggtctcc tgcgccgcgt ccacggcgtt gcgggtgagg
     3721 ttgatcagga gctgctcgag ctggtcggcg tcggcctcga tcgtcaggtc cggccccggg
     3781 cgcacggcga tggaggtgcg cgtctcgagc ccggcgacgc gccggacgat cgtcccgatc
     3841 gcgaccgggg cgaggcgcgg tggcggcagc cgggccaggc gcgcgtacgc gtccatgaag
     3901 cggccgagcg actcggcgcg cgtcgcgatg atcgacagcc cgccacgggc gtcctcccgc
     3961 cagtcggacg gcagcctctc ggcgtggagc agggtccgca ggctgtcggc aatcgagcgg
     4021 atcggcgcca gcgagttgtt catctcgtgg ccgacgacgc ggatgagccg ctgccaggcc
     4081 tggcgctcct cgtcgcgcag cgcccggctg acgtcggcga gcaccagcag ccggtgcggc
     4141 acgcccccct gccggaacgt cccggtgcgc gcctcgtagc gcccggagcc gcccgcgaag
     4201 gcgagatcga ggatgcgcgc tccggcgacc ccttgaggga cgcctccccc ctcggtgaag
     4261 catgcgtaca gcccgagcga ggcgatgtcg cgccccagga gctgctccgc cgggcggccg
     4321 atcaggcgct ccgccgcccg gttggcgagc cgcagccgcc cctcgtcgtc gaaggcgaag
     4381 atcccgacgt cgatctcgtc gatcaccttg cgcaggagcg cccccgcctc gagggccccg
     4441 aggcgctgct cgcgcagggt gtcgccgagg acgttgatct cgcgcatcac gtcctcgagg
     4501 gggtcatcgg ggcctctccc ccggccgcgc agcgagaagt ccccctcccc gagcgccgca
     4561 aggagattcg cgacgctctg cagcgggcgc agcacccgct cgcgcgccgc gaaggcgaac
     4621 atcatccacg gcaggatgac gaggagcgag aaggtgatct gggtcttgag ggcgaactcg
     4681 ccgagccaca gcaggaagag cgcgccgatc gtgccgggaa ggccggcgca cagggcgagg
     4741 gcgaggacga gacggtcgag ggtgaggcgc cgggtcacaa cccgtgccgc tgcaggcggc
     4801 gatagagagc gctgcggctc aatccgagcg ccgtggccgc ctggctgacg ttgccgccgt
     4861 gccgctcgag cgccttgcgg atcagcagac gctcgacgtc ctcgagcggg agatcct
//
LOCUS       DASRIQ010000142         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_830228, whole genome shotgun sequence.
ACCESSION   DASRIQ010000142 DASRIQ010000000
VERSION     DASRIQ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830228"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFD06_03495"
     CDS             <1..357
                     /locus_tag="VFD06_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02633.1"
                     /translation="SLSRDDAALRRAPANRDGFPYVEVASAATQAGRRAVGAGPFVRA
                     PLRRWLEALAARPIAGTPLARLDAEHLRWRSAGLAIWTASLLDLEGDRAGADALGLGG
                     VAMLPDELQKVIRGAR"
     gene            742..2124
                     /locus_tag="VFD06_03500"
     CDS             742..2124
                     /locus_tag="VFD06_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02634.1"
                     /translation="MRKSVRRCGTVLFLAVVIAFLPLGTARAEESAEVAALRQEVVAL
                     RAEIEALKAAMRELQQGAAGATSPAAETAPPAAGAPSAAAGAATAPPPLAPSAAPARS
                     ANVMNPAISAVFQAIGQSFLDGREDANGFDLSEAEVAFESTVDPYVKMNLYLTFPVDE
                     SPEVEEGYAQTLALPAGLQLKGGRFKESFGKWNLLHTHAFPTVERPDALVNFFGEESL
                     TNDGLSLSWLIPNPAGLYLESVTEAGTAREGPSFNSEARALTWSQHLSAFFNPSDNAT
                     LEFGLSGAFGQTGPTETLLADLDAAGLTGILVPDERTPSNVYGADVTWKWKPLSANTY
                     RSLTAQAEVLRSRRHAQTLSGASLDEEILTSDGGYLYLEGQWIKRWKIGLRGDLAGFP
                     DSETDRTWSAAGVVKFAPSEFQEIRFQVKRTRLNPGAAARAGGDGDQEDTQVFFEWIP
                     IIGAHGAHKY"
     gene            2136..3257
                     /locus_tag="VFD06_03505"
     CDS             2136..3257
                     /locus_tag="VFD06_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HZN02635.1"
                     /translation="MTCSVRSASRTRPAALLRVASVLLATALLPGSAHASGTVKVVAS
                     LPNLGAIARDIGGDRIDLTVIASGVQDAHFVDPKPSYIVKLRAADLLIVNGLDLEVGW
                     IPPLTQGARNGKVLPGGSGYVDASTGIPVVEIPAGLNRAEGDVHPYGNPHYLGDPLNA
                     LTVATTIAEALKKADPAGAAAYDERRKAFAARLDQALFGASLVELAGGSKLEREAAAG
                     TLDHFLDATSIGGAPLRTRLGGWLGRLAPFRGRPVVTYHKDASYFAARFGLVVADYVE
                     PKPGIPPSARHLEELTERLKHGEARVIVTRPYVEHRSTDLLAQKTGVAVVTLPLEAGG
                     DPKATDYFALFDLVTDRLAAALAPTGAGPAAPKKGSGSR"
BASE COUNT          482 a         1224 c         1132 g          465 t
ORIGIN      
        1 tcgctgtcgc gcgacgacgc ggcgctccgc cgtgcgccgg cgaaccgcga tggatttccc
       61 tacgtcgaag tcgcgagcgc ggcgacgcag gccggccggc gcgcggtcgg ggcgggcccg
      121 ttcgtgcgcg cgccgctccg gcgctggctg gaggcgctcg ccgcgcggcc gatcgccgga
      181 acgccgctcg cccggctgga tgcggagcac ctgcgctggc gctctgccgg gctcgccatc
      241 tggacggcgt cgcttctcga cctcgaaggg gaccgcgcgg gcgccgacgc cctcgggctc
      301 ggaggcgtgg cgatgctccc ggacgagctg cagaaagtga tccgaggggc gcgttaggcg
      361 cgatgctggc tgcggcacgg cgctgcaccc aggcgtcccc tgatggccac gcggatgcgg
      421 tcgccgcgag gatcacgcca tcaggagacg cgctgggctg agtcgcctgc accacttggc
      481 tgctcggcag agcaagccac gggccagcgc gacacatcca tcgctccggc gccgaagttc
      541 atcgtggagc acgagttgcg aggcggacgc cgctcccatc gccgacagcc ccatgcatcc
      601 tttttacgcg aaccggacgc gggacgcatc cacgtgatgt gggctgccta ggtccgacgc
      661 gccgggccaa cggcggtgga ggtttttgac tccccccagg ccgtgcagta gcatcccggc
      721 ccgcctgcag ggggcgatca aatgcgcaag tcggtccggc gttgcgggac ggtcctgttt
      781 ctggcggtcg tgatcgcgtt tctcccactc ggcacggccc gggcggaaga gagcgccgag
      841 gtcgccgcgc tgcggcagga ggtggtggcg ctgcgcgcgg agatcgaggc cctgaaggcc
      901 gccatgcggg agctgcagca gggggctgcg ggagccacgt cccccgccgc ggagaccgcc
      961 ccgccggccg cgggggcgcc gtcagccgcc gcgggcgccg ccaccgcgcc gccgccgctc
     1021 gccccatcgg cggcccccgc ccgctcggcg aacgtcatga acccggcgat cagcgccgtc
     1081 ttccaggcga tcggccagag cttcctcgac ggccgcgagg acgcgaatgg attcgatctc
     1141 agcgaggcgg aggtcgcctt cgaatcgacc gtcgatccgt acgtcaagat gaacctctac
     1201 ctgacgttcc cggtggacga gtcccccgag gtcgaggagg gctatgcgca gaccctcgcc
     1261 ctcccggccg ggctgcagct caaggggggt cggttcaagg agtcgttcgg caagtggaac
     1321 ctcctgcaca cccacgcctt cccgacggtg gagcgtcccg acgcgctcgt caatttcttc
     1381 ggcgaggaga gcctgacgaa cgacggcctg tcgctgtcgt ggctcatccc gaatccggcc
     1441 ggcctctacc tcgaatcggt gaccgaggca gggacggcgc gcgaaggacc ctcattcaac
     1501 agcgaggcgc gggccctcac ctggagccag catctctccg ccttcttcaa tccgagcgac
     1561 aacgcgaccc tcgagttcgg cctcagcggc gcgttcggac agaccgggcc gaccgaaacg
     1621 ctcctcgccg atctcgacgc cgccggcctg accggcattc tcgtccccga cgagcggacg
     1681 ccgtcgaacg tgtacggcgc cgatgtgacc tggaagtgga agcctctgag cgccaacacc
     1741 taccgctccc tgacggcgca agcggaggtg ctccgcagcc gccggcacgc gcagactctg
     1801 tccggcgcga gcctcgacga agagatcctc acgagcgacg gcggctacct ctatctcgaa
     1861 gggcagtgga tcaaacgctg gaagatcggg ctgcgtgggg acctcgcagg gttccccgac
     1921 agcgagaccg atcgcacctg gtcggccgcc ggagtcgtga agttcgcgcc gtcggagttt
     1981 caggagatcc gcttccaggt gaagcgcacc cgcctcaacc ccggcgccgc ggcgcgggcc
     2041 ggcggcgacg gcgaccagga ggacacgcag gtgttcttcg agtggattcc gatcatcggg
     2101 gcgcacggcg cccacaagta ctaggagctt cggcgatgac ctgctcggtc cgctccgcct
     2161 cgcgcacgcg ccccgcggcc ctcctccgcg tcgcgtccgt cctcctcgcc acagcgctcc
     2221 ttcccgggtc ggcgcacgcc tccggcaccg tcaaggtggt cgcctccctc cccaacctcg
     2281 gcgccatcgc gcgcgacatc ggcggcgacc ggatcgacct gaccgtcatc gccagcggcg
     2341 tgcaggacgc ccacttcgtc gatccgaagc cgtcctacat cgtcaagctc cgggccgccg
     2401 atctgctgat cgtcaacggg ctcgacctgg aagtcggctg gatcccgccg ctcacccagg
     2461 gggcgcgcaa cggcaaggtc ctcccgggcg gctccggcta cgtggacgcc tcgaccggaa
     2521 tcccggtcgt ggagatcccg gccggcctga accgggcgga gggggacgtc cacccctacg
     2581 gcaacccgca ctatctgggc gatccgctga acgcgctcac cgtcgcgacc acgatcgccg
     2641 aggcgctcaa gaaggccgac ccggccggcg ccgccgccta cgacgagcgc cggaaggcct
     2701 tcgccgcccg cctcgaccag gccctcttcg gcgcctcgct cgtcgagctc gccggcggct
     2761 ccaagctgga gcgggaggcg gcggccggga cgctcgatca cttcctcgac gccacctcga
     2821 tcggcggcgc accgctgcgc acgcggctcg gcggctggct cggcaggctc gccccgttca
     2881 gggggcggcc ggtggtcacg taccacaagg acgcgtcgta tttcgccgcc cgcttcggac
     2941 tcgtggtcgc cgactacgtc gagccgaagc cggggatccc gccgtccgcc cggcacctcg
     3001 aggaattgac ggaacgcctg aagcacggcg aggccagagt gatcgtgacc cgaccctacg
     3061 tcgagcaccg ctcgaccgat ctgctggccc agaagaccgg tgtcgcggtc gtcaccctgc
     3121 cgctcgaggc gggaggggat ccgaaggcca ccgactactt cgccctgttc gatctcgtga
     3181 ccgaccggct cgcggcggcg ctcgcgccga ccggggccgg gcccgcggcg ccgaaaaagg
     3241 gatccgggtc gcggtgatcc cgcaattcga agtcctgttc ctgccgttcc tcgcctgcct
     3301 cat
//
LOCUS       DASRIQ010000143         5255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2932261, whole genome shotgun sequence.
ACCESSION   DASRIQ010000143 DASRIQ010000000
VERSION     DASRIQ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2932261"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3384)
                     /locus_tag="VFD06_03510"
     CDS             complement(<1..3384)
                     /locus_tag="VFD06_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN02636.1"
                     /translation="MLLNGRWEVERRLGEGGMGEVFLARDRHHAGRAVALKILRPAAL
                     DDEALEHFRDEFRSLARLRHPNLVEVFDFGQVDDTGAPFLTQELVDGPSLAALPAATR
                     REQFESLAAQALRALDAIHTAGWLHNDLKPQNILITPPLRVRLVDFGLARRISAEGPA
                     APSGTLHYLAPERLRAGDPDPRSDLYALGAVLYECLTGRTPFEGKSAGALVTAILDGR
                     LPTPRSLDPAIPERWERFVLRLLARDPDARPRSAEEAIDALNAGARAQQPRDTPETFA
                     GRIRSAPLTARDAEMRALEAAIRTHLAPSQADRASGTVASAADGTRLVILSGPAGSGK
                     SRLAEEARQRLQLAAGRTLAGRAHEVGGHALQPFDQMLAAARAAGRMPAAIEPALEQA
                     FGAPTGRRNAARLERTDLVAALARGLDLLAEAQNGVLFLEDLQWASPLAIDLMEHLVA
                     RAEQSPWLLVATLRDGDAAAPVGRWQALARRARVASIPLPPLAKDGLVRILAAMLPFE
                     TPPERLAERLHTLNLSQPWHVEEVLKSLFQERLLEHRGGTWAPTAAALRDLPVPETIA
                     DLALARFAALPPGPADAARMLAIFGKPTALPTFARALQIAAPRGRPREEAPLALETLA
                     NLGLATLTWGDDGVPRAALAQSGMHAALARRLPAAARRALHKAALRAIEATEPEAARE
                     RVEDLAHHAVEAGERKSAATFLLAAARRAEALFDARGEATYYMKALEVLPLSDRKTRL
                     ETAGALCEAMSGKVGDFKQGARIARSLTSEARRAGVTWAEIKGLRFESFALGFLGETA
                     PAERLARKAVALARSAGEPGELGASLAFLGVLLARTGRPAEAAALFDEAVPLARAEGE
                     TRRLQWILNNAALTRLVLGETRASLERFEEILEVSRRTGANTSHHRALGNIGIARFDL
                     GDLEGAIEAVEIALAWGREHTELDQANEGARSLQQIQTVRGRFDLAAAAGREARRLCD
                     RLGQPADDPEAFELDGRVELGLGRFAGAIRLFRQGAEEARRRRDPVQEMYLSIALAEG
                     CVAAGEDAEATEHAARALAAARKLGHKRGLLLARRAQGLLALVAGSEAPIASGSDSVD
                     ASVTSVTNLIESIERDGEADLRFHDRLERGLLLGALDLKR"
     gene            complement(3473..4444)
                     /locus_tag="VFD06_03515"
     CDS             complement(3473..4444)
                     /locus_tag="VFD06_03515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN02637.1"
                     /translation="MGEVVQMPEGAGIAGMLVAAGVITCALLLAWLCVHALRDLGRTP
                     QRNLADRLKSVSATVGDLDAYVALVRALAEMAPRRTAQGSFADEIRALALDLAAKLER
                     PDQEIEWLQTSLPPDPTGLPATAAPPAAVARLRGLLDRGAAGRGQEEEPAEGASESRL
                     LFVSFEREIRDAERRGTPLSLIDLRVEGFETIEERFGRPATDRILRGVARAIRSQLRP
                     GDTCIRDAGGSFLILVPGLAAEAVATLSSRIEAAVSRHKFAPERGRSLRLEASLCAAT
                     FPRDGGSYDALITAARSRRALKQPASSRDDVPAGFRPYPNRIDAPVN"
     gene            4666..>5255
                     /locus_tag="VFD06_03520"
     CDS             4666..>5255
                     /locus_tag="VFD06_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02638.1"
                     /translation="MPAVGLLLAVVILLILSFVRIAPGERAFRVSGDGAQPLAPGMHL
                     RFPGRIVRLAAGTISVRGEVPLRSSEGAALAATYEVSAGVPDAAVAKVLAQAPGPGDL
                     EGSLRRAAVAGVEKWAQGRSGEAIALGEGRAAAEDAIRRRLGEEGYDPVQVRIAGAPG
                     SSEMQAKLAAQAVRQRSEATGLKVAILGLDGADWEIL"
BASE COUNT          695 a         1912 c         1964 g          684 t
ORIGIN      
        1 ccttttcaga tcgagcgcac cgagaagcaa ccctcgttcc agccgatcgt ggaatcgcag
       61 gtcggcttcg ccgtcacgct cgatcgattc gatgagattt gtgacggagg tcaccgaagc
      121 atccacggaa tcggatccgg acgcgatcgg tgcttcggat cctgcgacca gggccagaag
      181 cccctgcgcc cgccgggcca gcaagaggcc tcgtttgtgg ccgagcttcc gcgccgccgc
      241 gagcgcgcgc gccgcgtgtt cagtcgcctc ggcatcctcg ccggcggcga cgcagccctc
      301 ggccagggcg atcgacaggt acatctcctg gaccgggtcg cgcctcctcc gggcttcctc
      361 cgctccctgg cggaacagcc ggatcgcccc ggcgaagcgt ccgagcccca actcgacgcg
      421 cccgtcgagc tcgaacgcct ccggatcgtc cgccggctgg cccaggcggt cgcagagccg
      481 ccgcgcttcg cgtcccgccg ccgcggcgag atcgaagcgg ccgcgcaccg tctggatctg
      541 ctgcagcgac cgcgccccct cgttcgcctg gtccagctcg gtgtgctccc gtccccacgc
      601 cagcgcgatc tcgaccgcct cgatcgcccc ctccagatcg ccgagatcga accgggcgat
      661 cccgatgttc ccgagggcgc ggtggtggga ggtgttcgcc ccggtccggc gcgacacttc
      721 gaggatctcc tcgaagcgct ccaggctcgc ccgcgtttca cccaggacga ggcgcgtcag
      781 ggccgcgttg ttcaggatcc actggaggcg ccgcgtctct ccctccgccc gggcgagcgg
      841 caccgcttcg tcgaaaagcg ccgccgcctc ggccggccgg ccggtgcgcg ccaggaggac
      901 tccgagaaac gcgaggctcg cccccagctc gcccggctcg ccggcggagc gggccagggc
      961 gaccgccttg cgggcgagcc gctcggccgg ggccgtctcg ccgagaaagc ccagcgcgaa
     1021 cgactcgaac cgcagaccct tgatctcggc ccaggtcacg ccggcgcggc gcgcttcgct
     1081 cgtgagcgat cgggcgatgc gggccccctg cttgaagtct ccgacctttc ccgacatcgc
     1141 ctcgcacagc gcgcccgccg tctcgagccg cgtcttgcgg tccgagaggg gcaggacctc
     1201 gagcgccttc atgtaatagg tcgcctcgcc gcgcgcgtcg aacagcgctt cggcgcggcg
     1261 cgccgcggcc agaagaaaag tcgccgcgct cttgcgctcg cccgcctcga cggcgtggtg
     1321 cgcgagatcc tcgacccgct cgcgcgccgc ctccggctcg gtcgcctcga tggcgcgcag
     1381 cgccgccttg tgcagcgccc gccgcgccgc cgcggggagg cggcgggcga gggcggcgtg
     1441 catcccgctc tgcgcgagcg cggcacgcgg cacgccgtcg tccccccagg tcaaggtggc
     1501 gaggccgaga ttcgcgagcg tctccagcgc caggggcgcc tcctcgcgtg gccggccgcg
     1561 cggagcggcg atctgcagcg cgcgcgcgaa cgtcggaagc gccgtcggct tcccgaagat
     1621 cgccagcatg cgggcggcgt ccgccggtcc gggcggcagg gccgcgaagc gggcgagggc
     1681 gaggtcggcg atcgtctccg ggaccggcag gtcgcgcagc gccgccgccg tcggcgccca
     1741 ggtgcccccg cgatgctcca ggagccgttc ctggaagagc gacttgagga cctcctcgac
     1801 gtgccacggc tgcgacaggt tgagcgtgtg cagacgctcg gccagccgct ccggcggcgt
     1861 ctcgaacggc agcatcgccg cgaggatccg caccagcccg tccttcgcga ggggagggag
     1921 cgggatcgag gcgacgcgcg cccggcgcgc cagcgcctgc cagcgcccca cgggcgcggc
     1981 ggcgtcgccg tcgcgcagcg tcgcgaccag cagccacggc gactgttccg cgcgcgcgac
     2041 gaggtgctcc atgaggtcga tcgccagggg cgaagcccac tggagatctt cgaggaacag
     2101 gacgccgttc tgcgcctccg cgaggagatc gaggccgcgc gccagcgcgg cgaccaggtc
     2161 ggtgcgctcc agccgcgccg cgttccggcg gccagtcggc gcgccgaacg cctgctcgag
     2221 ggccggctcg atcgcggccg gcatccgtcc ggcggcgcgc gccgccgcga gcatctggtc
     2281 gaacggctgc agcgcgtggc cgccgacctc gtgcgcgcgt ccggcgaggg tgcggccggc
     2341 ggcgagctgc agccgctggc gcgcctcttc ggcgagccgg ctcttcccgg atcccgcggg
     2401 accgctcagg atcaccagcc gcgttccgtc ggcggcgctc gccacggtgc cgctcgcgcg
     2461 gtcggcctgc gatggcgcga ggtgggtgcg gatcgccgcc tcgagcgccc gcatctcggc
     2521 gtcgcgcgcg gtgagcggcg cggagcggat ccggccggcg aaggtctcgg gagtgtcacg
     2581 cggctgctgc gcccgggcac cggcgttgag cgcgtcgatc gcctcctcgg cggagcgcgg
     2641 gcgcgcgtcc ggatcgcgcg ccaggaggcg caggacgaag cgctcccagc gctcggggat
     2701 ggcggggtcg aggcttcgcg gcgtcggcag tcgtccgtcg agaatcgcgg tgacgagcgc
     2761 gccggcgctc ttcccctcga acggcgtccg cccggtcagg cactcataaa gaacggcgcc
     2821 gagggcgtag agatcggatc gcgggtcggg atcccccgcc cgcagacgct ccggggcgag
     2881 gtagtgcagc gtgccgctcg gcgccgccgg cccctcggcg ctgatgcgcc gcgccaggcc
     2941 gaaatcgacg agccgcacgc gcagaggcgg ggtgatcagg atgttctgag gcttgaggtc
     3001 gttgtgcagc cagcctgcgg tatggatcgc gtcgagggcg cgcagcgcct gggcggcgag
     3061 cgactcgaac tgctcgcggc gcgtcgcggc ggggagggcg gcgagcgacg ggccgtccac
     3121 cagctcctgg gtcaggaacg gggcgccggt gtcgtccacc tggccgaagt cgaacacctc
     3181 gacgaggttc gggtggcgca gtcgcgcgag cgatcggaac tcgtcgcgga aatgctcgag
     3241 cgcctcatcg tccagcgccg cggggcggag gatcttgagc gcgacggcgc gcccggcgtg
     3301 gtgccggtct cgggcgagga acacctctcc catgccgccc tcgccaagac ggcgctccac
     3361 ctcccatcgc ccgttcagca gcaactcgaa tccgctcaaa aagttggctg gaaactacac
     3421 ccgttgccgg ggggccgcaa gtggagacga tccgacgttt ttctgacgga cgctagttga
     3481 ccggtgcgtc gatgcggttg gggtaggggc gaaacccggc agggacgtcg tcgcgcgacg
     3541 aggcgggctg cttcagggcc cggcgcgaac gggccgcggt gatgagggcg tcgtaggagc
     3601 cgccgtcgcg cgggaaggtc gcggcgcaga gactggcctc gagccgcagg gatcggccgc
     3661 gctcgggggc gaacttgtgg cgcgacaccg ccgcctcgat gcgcgacgag agggtggcga
     3721 ccgcttcggc cgccaggccc ggcacgagga tgaggaagga gccgccggcg tcccggatgc
     3781 acgtatcgcc tgggcggagc tggctgcgga tcgcccgtgc cacgccgcgc aggatccggt
     3841 cggtcgcggg ccggccgaag cgctcctcga tcgtctcgaa tccctcgacc cgcagatcga
     3901 tcaggctgag cggcgtcccg cgccgctccg cgtcccggat ctcccgctcg aagctgacga
     3961 acagcagccg gctctcggag gccccttccg ccggctcctc ctcctgcccg cggccggccg
     4021 cgccgcggtc cagcaggccg cgaagacgcg cgacggccgc cggaggagcc gccgtcgccg
     4081 gaagaccggt cgggtcgggc gggagagagg tctgcagcca ttcgatttcc tggtcaggac
     4141 gctcgagctt cgccgccagg tcgagcgcca gagcgcggat ctcatcggcg aacgacccct
     4201 gggccgtgcg ccgcggcgcc atctcggcga gcgccctcac gagggcgacg taagcgtcga
     4261 ggtcgcccac ggtggccgaa acgctcttca ggcgatcggc caggttgcgc tgaggggtgc
     4321 gacccaggtc gcggagggcg tggacgcaga gccaggcgag gagcagcgcg caggtgatga
     4381 cccccgcggc gaccagcatt cccgcgattc ccgctccttc cggcatctgg acgacctcgc
     4441 ccacggaaga tcggccgccc ggcgccggcc gtccatttcg gtttcgaaac cgccggcgtg
     4501 tcggtcggca ggccgcgtca ctggggcagc acgaaagccc gcgggtcttg ccattcagag
     4561 gcgtgccccc tagcatcctt cttctgttcg ccgggagcgc ctgacgtgaa gcagcggaaa
     4621 cgggacgtcc cacccccccc agaaaggaag gggcggaagg ggtgggtgcc ggcggtcggc
     4681 ctcctcctgg cggtcgtcat ccttctgatc ctttccttcg tcagaatcgc tccgggagag
     4741 cgggccttcc gggtatcggg ggatggcgcc cagccgctcg ctcccggcat gcacctccgg
     4801 ttcccggggc gcatcgtccg gctggccgcc gggaccatct cggtccgcgg cgaggtgccg
     4861 ctgcgatcga gcgaaggagc ggcgctcgcc gcgacctacg aggtcagcgc gggggtgccg
     4921 gacgcggcgg tggcgaaggt gctggcgcag gcgcccggcc cgggcgacct cgagggctcg
     4981 ctccgccgcg cggccgtcgc cggcgtcgag aagtgggcgc agggacgatc cggcgaggcg
     5041 atcgcgctcg gcgagggacg cgcggcggcc gaggacgcga tccgccgccg gctcggcgag
     5101 gaagggtacg acccggtcca ggtgcggatc gccggcgccc ccggctcgtc ggagatgcag
     5161 gcgaagctcg cggcccaggc ggtgcgccag agaagtgaag cgaccgggct caaggtcgcg
     5221 atcctcggcc tcgacggcgc cgactgggag atcct
//
LOCUS       DASRIQ010000144         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3176749, whole genome shotgun sequence.
ACCESSION   DASRIQ010000144 DASRIQ010000000
VERSION     DASRIQ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3176749"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFD06_03525"
     CDS             complement(<1..737)
                     /locus_tag="VFD06_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN02639.1"
                     /translation="MSSSRKWIVTILALAGLAAAGAAVARRDASKDDGGIRAETVGRG
                     EVAATVTATGTISAVTTVQVGSQVSGIVSSLSVDYNSPVTKGQLLATLDPTPFEAQVE
                     QRRADLLRAEVEARNAEVAFRRQERLLDQGLTAPSDYDTAKAAFDAAQAGVAQAEATL
                     RQARTNLSYTRIYSPIDGVVVARQYDIGQTVAASFQAPTLFTIAEDLTKMQVQADVDQ
                     SDIGRVAMGQTARFTVDAYADETFSGT"
     gene            complement(784..1116)
                     /gene="trxA"
                     /locus_tag="VFD06_03530"
     CDS             complement(784..1116)
                     /gene="trxA"
                     /locus_tag="VFD06_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZN02640.1"
                     /translation="MSSEKILTLTTGNFDDETKKAGAPLLVDFWAEWCGPCRMLAPVL
                     DQLANDYDGRVRVGKLNVDDHSSIATRFGVMSIPTLLLFKEGKVVEQYIGSTSKDNLA
                     KLIEKHLA"
     gene            1212..2081
                     /gene="rsmA"
                     /locus_tag="VFD06_03535"
     CDS             1212..2081
                     /gene="rsmA"
                     /locus_tag="VFD06_03535"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZN02641.1"
                     /translation="MRLGPEGFRPKRRWGQNFLVDPAALQAIAAAFRPAPTDRVLEIG
                     PGDGALTRRIAPRVFSLTAVEVDPELAASLRTSLEEVRIVEADVLALDPTALLRDAGA
                     SPGTPARLLGNLPYNIATAVILRFLNRHDLVRDLMVMVQREVAERILSAPHRKEFGSL
                     SVLCRTFARVERVRRLRPGSFRPRPRVESEVIHLTLCDPGGAAGAEPDRFAAFVQGCF
                     EQRRKTFQNNLARLLARSGGGADDRARAFLEAVGIAPRGRPEEVPPEGFQRLFEALEA
                     ERSGRAPAPSPPV"
     gene            2090..3781
                     /locus_tag="VFD06_03540"
     CDS             2090..3781
                     /locus_tag="VFD06_03540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZN02642.1"
                     /translation="MTTRHRGEAPRQPQQRLEVIPLGGLGEFGLNCTVLRSGNDLLVI
                     DAGVMFPEETILGVNVVIPEFAWLFERAEHVHGILLTHGHEDHVGALPYLFEKVRVPV
                     YGSDFTLGLASRRLKEHGLPTEKLLKPVQAGTEIPLGPFVVEFLQVTHSIPGTLAIAI
                     RTPAGTVIHSADFKMDQTPIDGRTFDFQSFSYYGDQGVLALLSDSTNAEVEGFTGSER
                     LVGEAIDGVFRRAQGRILFSTFASNVHRLQQVIDSAVMHKRRVVLCGTSMSSVSTVAQ
                     ELGYLKVPAGVLAETSEARRIPPNRLVILAAGSQGEPMSALSRIALDDHRDVSIEPGD
                     TVLVSARAIPGNERAINRVVNHLYRRGAEVVFGSAPPYHVSGHASQEELKLLLTLVRP
                     KYFIPIHGEIRQLYSHARLAERTGLDRRRILLAESGDVIELNGDGASITGKVRAGRVF
                     IDGTLDEVDEIVVRDRRHISEGGIVLVVVAIDKQSGQMEGEPEILSRGFVIETERGDL
                     LREANRAVRRAVEAATPEERADRGVLKAMIQQELKRFFRKRLDKRPMIIPAVVET"
     gene            3801..>4176
                     /locus_tag="VFD06_03545"
     CDS             3801..>4176
                     /locus_tag="VFD06_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZN02643.1"
                     /translation="MSRRRRKSPAKRHEAAAASRGILAEGLAILLMATAVLLALSLVS
                     HRESDPVPWLGQWEGGEPVRNIAGIVGSFLSALLYQTFGWAAWSAPLLMLFLGWRVFW
                     HRPSPFVGTAGSAVVLVAFTAIL"
BASE COUNT          579 a         1471 c         1471 g          655 t
ORIGIN      
        1 atcgtaccgc tgaacgtctc gtcggcgtag gcgtccaccg tgaagcgggc ggtctgcccc
       61 atcgccacgc gcccgatgtc ggactgatcg acatcggcct gcacctgcat cttggtcagg
      121 tcctcggcga tggtgaacag tgtcggcgcc tggaacgacg cggcgaccgt ctggccgatg
      181 tcgtactgcc gcgccaccac cacgccgtcg atcggcgaat agatcctggt gtaggagagg
      241 ttcgtgcgcg cctgccgcag cgtcgcctcc gcctgggcca cccccgcctg cgccgcgtcg
      301 aacgccgctt tggcggtgtc gtagtcggag ggggcggtca gaccctgatc gagcagccgc
      361 tcctggcggc ggaaggcgac ctcggcgttg cgcgcttcga cctcggcgcg caggagatcg
      421 gcgcggcgct gttccacctg cgcctcgaac ggcgtggggt cgagggtggc gaggagctgc
      481 cccttcgtca ccgggctgtt gtagtccacc gagaggctgc tgacgatgcc cgagacctgg
      541 cttccgacct gcaccgtggt gaccgcggag atggtgccgg tggcggtgac ggtcgcggcc
      601 acctcgcccc gcccgaccgt ctcggcgcgg atgccgccgt cgtctttgga cgcgtcgcgg
      661 cgcgccaccg cggcccctgc cgcggccagc ccggcgagcg cgagaatggt gacgatccac
      721 ttgcgggagg aagacatggc ttgcgttccg ctcccgcccc cggcgcggga gccgccggac
      781 cttctatgcg aggtgctttt cgatcagctt ggcgaggttg tctttcgagg tggagccgat
      841 gtactgctcg accaccttcc cctccttgaa gaggaggagg gtggggatgc tcatcacgcc
      901 gaaccgggtg gcgatgctgc tgtgatcgtc gacgttgagc ttgccgaccc gcacgcggcc
      961 gtcgtagtcg ttcgccagct ggtcgagcac cggcgccagc atgcggcacg ggccgcacca
     1021 ctcggcccag aagtcgacca ggagcggggc tccggccttt ttcgtctcgt cgtcgaagtt
     1081 ccccgtcgtc agggtgagaa tcttctcgct gctcatcaga aatgagacct cccgtctcgt
     1141 tccactcgtt caacggcgcc acctgccgga cccattttta taccatactc ggcgccgggg
     1201 gacttctttg gatgcgcctc gggcccgagg gattcagacc gaaacgacgc tggggacaga
     1261 acttcctcgt cgaccccgcc gcgctgcagg cgatcgccgc ggcgttccgc cccgcgccga
     1321 cggaccgggt gctcgagatc ggccccgggg acggcgccct gacccgccgc atcgccccgc
     1381 gcgtcttcag cctgaccgcc gtcgaggtcg atcccgagct cgcggcgtcc ctccggacgt
     1441 cactcgagga ggtgcggatc gtcgaggcgg acgtcctggc gctcgacccg acggcgctcc
     1501 tgcgggacgc cggcgcttcg cccgggacgc cggcgcggct cctcgggaac ctgccgtaca
     1561 acatcgccac cgccgtcatc ctccgcttcc tgaaccggca cgacctcgtc cgcgacctga
     1621 tggtgatggt gcagcgcgag gtggcggagc ggatcctctc cgcgccgcac cgcaaggagt
     1681 tcgggagcct gtccgtcctc tgccggacgt tcgcccgggt ggagcgggtg cgccgtctcc
     1741 ggcccgggtc gttccggccg cgcccgcgcg tcgagtcgga ggtcatccac ctgacgctgt
     1801 gcgacccggg gggcgcggcc ggggccgagc cggaccggtt cgccgccttc gtccagggct
     1861 gtttcgagca gcggcgcaag acgttccaga acaatctggc gcgcctcctg gcgcggtccg
     1921 gtggcggcgc cgacgaccgc gcccgggcgt tcctcgaggc ggtggggatc gcgccgcggg
     1981 gacggccgga agaggttccg ccggaggggt tccagcgcct gttcgaagcg ctcgaggcgg
     2041 agcgctccgg tcgggcgccg gcgccgtccc cccccgtata atgcgttccg tgaccacccg
     2101 ccaccgcggc gaggcaccgc ggcagccaca acagcgactc gaggtgatcc cgctcggcgg
     2161 tctgggcgag ttcggcctca actgcaccgt cctgcgatcc gggaacgatc tcctcgtgat
     2221 cgacgccggg gtgatgttcc cggaggagac gatcctcggt gtgaacgtcg tcatccccga
     2281 gttcgcgtgg ctgttcgagc gggccgagca cgtccacggg atcctgttga cgcacggcca
     2341 cgaggaccac gtcggggcgc tgccgtacct gttcgagaag gtgcgcgtcc cggtctacgg
     2401 cagcgatttc accctcgggc tcgccagccg ccgcctgaaa gagcacggcc tgccgaccga
     2461 gaaactcctc aagccggtgc aggccgggac ggagatcccg ctcgggccgt tcgtggtcga
     2521 gttcctccag gtgacgcaca gcatcccggg gaccctcgcg atcgccatcc gcacgccggc
     2581 cggcaccgtc atccactccg ccgacttcaa gatggaccag acgccgatcg acggacggac
     2641 cttcgacttc cagtcgttct cctactacgg cgaccagggg gtgctcgccc tcctgtcgga
     2701 cagcaccaac gccgaggtgg aggggttcac cggctccgag cgcctggtcg gcgaggcgat
     2761 cgacggagtg ttccggcgcg cccagggacg catcctgttc agcaccttcg ccagcaacgt
     2821 gcaccgcctc cagcaggtga tcgacagcgc tgtgatgcac aagcgccgcg tcgtgctgtg
     2881 cggcacgagc atgagctccg tctccacggt ggcgcaggag ctgggatacc tcaaggtccc
     2941 cgccggcgtg ctggcggaga cctcggaggc gcgtcgcatc ccgccgaacc gcctggtgat
     3001 cctggccgcc gggtcgcagg gggagccgat gtcggcgctg tcgcgcatcg ccctcgacga
     3061 tcaccgcgac gtctcgatcg agccggggga cacggtgctg gtgtcggcgc gcgccatccc
     3121 ggggaacgag cgggccatca accgggtggt caaccatctc taccggcgcg gcgccgaggt
     3181 ggtgttcgga tccgccccgc cgtaccacgt ctccggccac gccagccagg aggagctgaa
     3241 gcttctgctc acgctcgtgc gcccgaagta cttcatcccg atccacgggg agatccgtca
     3301 gttgtacagt cacgcgcggc tggccgagcg gaccgggctc gatcggcgca gaatcctcct
     3361 ggcggagagc ggcgacgtca tcgagctgaa cggcgacggc gcctcgatca ccggcaaggt
     3421 gcgggccggg cgggtgttca tcgacggcac gctcgatgaa gtggacgaga tcgtggtgcg
     3481 cgaccgccgc cacatctcgg aaggggggat cgtgctcgtg gtcgtggcga tcgacaagca
     3541 gagcggccag atggagggag aaccggagat cctcagccgc ggcttcgtca tcgagacgga
     3601 gcgcggcgac ctgctgcgcg aagccaaccg ggcggtgcgg cgcgccgtcg aggcggccac
     3661 ccccgaggag cgggccgacc gcggcgtcct caaggcgatg atccagcagg agctgaagcg
     3721 cttcttccgg aagcgcctcg acaagcggcc gatgatcatc ccggcggtgg tggagacgtg
     3781 agcgaagcgc ggagcaggcg gtgagccgcc ggcgccggaa gagtcccgcg aagcgccacg
     3841 aggcggcggc cgcctcgcgc ggcatcctcg ccgaggggct ggcgatcctg ctcatggcca
     3901 ccgcggtcct cctggccctg agcctcgtct cccaccgcga gtcggacccg gtgccgtggc
     3961 tcgggcagtg ggaggggggg gagccggtgc gcaacatcgc cggcatcgtc gggtcgttcc
     4021 tctcggcgct gctgtaccag acgttcggct gggccgcctg gagcgcgccg ctcctgatgc
     4081 tgttcctcgg ctggcgcgtc ttctggcacc gcccgtcccc cttcgtcggc acggccggca
     4141 gcgccgtggt gctggtggcc ttcaccgcca tcctcg
//
LOCUS       DASRIQ010000145         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_831200, whole genome shotgun sequence.
ACCESSION   DASRIQ010000145 DASRIQ010000000
VERSION     DASRIQ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831200"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1924
                     /locus_tag="VFD06_03550"
     CDS             50..1924
                     /locus_tag="VFD06_03550"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HZN02644.1"
                     /translation="MNAQTRHSMLTPNPARRGEEGSALILAALVTVILSLLGLSYLMM
                     AQTENTIAENERNSAAALYVAEAGTRLVIGWFNDPSATGYLVPTLGDLDRTKRIFDHD
                     NNGGTARVQGASADAAKPLYKDATQTLSLIFDRPYRGVLADTLLGIETGTDPNVAFAA
                     MGPDLIVNASFLTTINDTLFPNFPTPFLRARITRIEVYAPPILSIGGSDTRMGVATVK
                     VTAGVFQYPGTASERQIATRIVKAVINEIPVPGPVGPLQSCSGLAYNGDFKIHWGTGT
                     SVGNADIPVAGQLDSKMLSGLPYALNDPFTYINGATTLVSWATAHTNEQVEDPWFKYI
                     AGGTIDSAGNTNPQPWPWPSSPDLDKEHSNLFQNTTVNCPTFDYALWKSIAQSGLKNN
                     YYYKWVSGTNYSLDGTGTSTDYRVLTNGKAGIFFFDTQDTLAPNVAGTNLTPAISLSG
                     SGYGVSGFVYVNAANYATTGLGGLMTTYFPPGEPSDASGFVNFDYPGSTADNYVIKQT
                     TGNFETFQDPVTNIWYCTDAATCTSASRTPAGAPVQDLTGLPFQTDAVLNGVLYNSGT
                     FETQGNAKYFGSVVGQRGVIDGGGTPEFWFDERLIKGEWPPKGMSIPRVVVSAWQTDL
                     "
     gene            2041..2934
                     /locus_tag="VFD06_03555"
     CDS             2041..2934
                     /locus_tag="VFD06_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02645.1"
                     /translation="MDRRLNRPVFAVALFLGGLAAAGGTPLRAADPAPPSDQETQDQA
                     KKNPKADPPRRFTDDDLEEKYHRKPQAQPAEEETGAPAAATAGVPAARPPAAPPAGAK
                     APAAGAPTKPPATATAAATKPPARPGPPPVKKVLSSPTAPTDPLQGYRVAAAKDEARE
                     RELKARRERIATLQSRLDYLQAKRNALVNPGATAVGSTGPLVYDYDETTGKPVMDPTT
                     GKPSTKPDISPGRATAPVSPIFPPLPPAQTDEDRENDRKMKVKDLLAAVEKEIPGVEE
                     ELARARQDLVDYETRFAAGAP"
     gene            2931..>4009
                     /locus_tag="VFD06_03560"
     CDS             2931..>4009
                     /locus_tag="VFD06_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN02646.1"
                     /translation="MKSADPILLVEDKAALRAVVRQTLEAEGFAVEEAADGQKAIDRL
                     RRERFALVLTDLRLPKASGHEVLQAAAGADPSTPVILMTAYGTIRDAVEAMREGAWDY
                     LEKPVDQDHLIALVRKALEHQTVVRENRALKERFARDLGGPEILGESAALRQSLEQVQ
                     RVAGTDATVLLLGESGTGKELFARAVHHQSPRRERPFLALNCAAIPDNLLESELFGYE
                     KGAFTGATASKRGKLEIADRGTLFLDEIGDLPPALQGKLLRVLEQKQFERVGGNDTRT
                     VDIRLVAATNKDLRALAGTPAFRQDLYFRLSVFPITIPPLRDRREDVTLLARHFAACF
                     AAEQKRRGPVTFDPAALDAMREYSWP"
BASE COUNT          686 a         1463 c         1325 g          535 t
ORIGIN      
        1 ttcgtggtcc atccggacct gtcgatcacg ggaccttaag ggggcgccga tgaacgcaca
       61 gacgaggcat tcgatgctga ccccgaaccc ggcccgccgc ggcgaggagg gatccgccct
      121 catcctcgcc gcgctggtga cggtcatcct gagcctcctc gggctgtcct acctgatgat
      181 ggcccagacc gagaacacca tcgccgagaa cgagcggaac tccgccgcgg cgctgtacgt
      241 cgccgaggcg gggacgcggc tcgtcatcgg ctggttcaac gacccgagcg ccaccggcta
      301 cctggtgccg acgctcggcg atctcgaccg caccaaacgg atcttcgacc acgacaacaa
      361 cggcgggacg gcgcgcgtgc agggggcgtc cgcggacgcc gccaagccgc tctacaagga
      421 cgcgacgcag accctgagcc tcatcttcga tcggccgtac cgcggcgtcc tggccgacac
      481 gctcctcggc atcgagaccg gcaccgatcc aaacgtggcc ttcgccgcca tggggcccga
      541 cctcatcgtc aacgcctcgt tcctgacgac gatcaacgac acgctcttcc cgaacttccc
      601 gacccccttc ctgcgcgccc gcatcacccg gatcgaggtg tacgcgccgc cgatcctgag
      661 catcggcggc tcggacacca ggatgggagt ggcgacggtg aaggtgacgg ccggggtctt
      721 ccagtacccg gggaccgcct cggagcggca gatcgcgacg cgcatcgtca aggcggtcat
      781 caatgagatc ccggtccccg gcccggtcgg gccgctgcag tcctgctccg gcctggcgta
      841 caacggagac ttcaagattc actgggggac ggggacctcg gtcggcaacg ccgacatccc
      901 ggtcgcgggc cagctcgaca gcaagatgct cagcgggctg ccgtatgcgc tcaacgatcc
      961 cttcacctac atcaacggcg cgacgacgct cgtgtcctgg gcgaccgcac acaccaacga
     1021 gcaggtcgag gacccgtggt tcaagtacat cgccggcggg acgattgact cagcggggaa
     1081 cacgaacccg cagccgtggc cctggcccag cagccccgat ctggacaagg agcacagcaa
     1141 cctgttccag aacacgaccg tgaactgccc gaccttcgat tacgcgctgt ggaagtcgat
     1201 cgcccagagc ggcctgaaga acaactacta ctacaagtgg gtgagcggca cgaactactc
     1261 gctcgacggc accggaacgt cgaccgatta ccgggtgctg accaacggca aggcggggat
     1321 cttctttttc gacacgcagg acacgctcgc gccgaatgtc gccgggacca acctgacgcc
     1381 ggcgatctcc ctcagcggca gcgggtacgg agtgtccggg ttcgtctacg tcaacgccgc
     1441 caactacgcc accaccggtc tcggcggcct gatgacgacc tactttccgc ccggcgagcc
     1501 gtcggacgcc agcggcttcg tcaacttcga ctacccgggg agcacggcgg acaactatgt
     1561 catcaagcag acgacgggga acttcgagac gttccaggat ccggtgacca acatctggta
     1621 ctgcaccgac gcggccacct gtacctccgc ctcgcgcaca ccggcggggg cgccggtcca
     1681 ggacctcacc ggcctgccgt tccagaccga cgcggtcctc aacggcgtgc tgtacaactc
     1741 cgggaccttc gaaacgcagg ggaacgccaa gtacttcgga tcggtcgtgg gccagcgcgg
     1801 ggtcatcgac ggcggcggta cgcccgagtt ctggttcgac gagcgcctga tcaaggggga
     1861 gtggcccccg aaggggatgt cgattccgcg cgtcgtcgtc tcggcctggc agaccgacct
     1921 gtaagagcgg gccggccgcc ggagccggcg gctccgggcc gtgcggttgc acgccgcacc
     1981 cccggaacct acatttccgt catccgctcg gtgccgagcg tgtgtccgcg ggagggagtc
     2041 atggatcgac gactgaatcg cccggtcttc gccgtcgcgc tgttcctcgg cggcctcgct
     2101 gcggccggtg gcacgccgct gcgcgccgcc gatccggccc cgccgtccga ccaggagacg
     2161 caggaccagg cgaagaagaa tcccaaggcg gatcctccgc ggcgcttcac cgacgacgac
     2221 ctggaggaga aataccatcg gaagccgcag gcgcagcccg ccgaggaaga gaccggggcc
     2281 ccggccgcgg ctaccgccgg cgtccccgcc gcgaggccgc ccgcggcccc gccggcgggg
     2341 gccaaggctc ccgcggccgg tgcgccgacc aagcctcccg caaccgcaac cgcggccgcg
     2401 acgaagccgc ccgcccggcc cgggccgccg ccggtgaaga aggtcctgtc ttccccgacc
     2461 gccccgaccg acccgctcca ggggtaccgc gtcgcggcgg cgaaggacga agcgcgcgag
     2521 cgcgagctga aggcgcggcg cgagcgcatc gcgacgctcc agtcgcggct cgactatctc
     2581 caggccaagc gcaacgcgct cgtcaatccc ggagcgacag cggtcgggag caccggaccg
     2641 ctcgtctatg actacgacga gacgaccggc aagccggtga tggacccgac gaccggcaag
     2701 ccttcgacga agcccgacat ctcccccggc cgcgccaccg caccggtctc gcccatcttc
     2761 ccgccgctcc ctccggccca gaccgacgag gatcgcgaga acgatcggaa gatgaaggtc
     2821 aaggatctcc tggcggcggt cgagaaggag atcccgggcg tcgaggagga gctcgcccgt
     2881 gcccgtcagg atctcgtgga ctacgagacc cgtttcgccg ccggcgcccc ttgaaatccg
     2941 ccgatcccat cctgctggtc gaggacaagg cggcgctgcg cgccgtcgtg cgccagaccc
     3001 tcgaggcaga ggggttcgcc gtcgaggagg cggcggacgg ccagaaggcg atcgatcgcc
     3061 tgcgccgcga gcggttcgcc ctcgtcctca ccgaccttcg ccttccgaaa gcgagcgggc
     3121 acgaggtgct gcaggcggcg gccggcgccg acccctcgac cccggtgatc ttaatgacgg
     3181 cgtacgggac gatccgcgac gccgtcgagg cgatgcgcga gggagcctgg gactacctcg
     3241 agaagccggt ggaccaggac cacctgatcg cgctcgtccg caaggcgctc gagcaccaga
     3301 cggtcgtgcg ggagaaccgg gcgctcaagg agcgcttcgc gcgcgacctc ggcgggccgg
     3361 agatcctcgg cgagtcggcg gcgctccggc agtcgctcga gcaggtgcag cgggtcgcgg
     3421 ggaccgacgc cacggtgctc ctcctcgggg agagcggcac cggcaaggag ttgttcgcgc
     3481 gggcggtgca ccaccagagc ccgcgccgcg agcgcccctt cctcgccctc aactgcgccg
     3541 ccatcccgga caacctgctc gagagcgagc tgttcggcta cgagaaaggg gcgttcacgg
     3601 gagcgaccgc cagcaagcgc ggcaagctcg agatcgccga tcgcgggacc ctgttcctcg
     3661 acgagatcgg cgacttgccg ccggcgctgc aggggaagct gctgcgcgtc ctggagcaga
     3721 agcagttcga gcgggtcggc ggcaacgata cccgcaccgt ggacatccgt ctcgtcgccg
     3781 ccacgaacaa ggacctccgc gccctggccg gcacacccgc ctttcgccag gacctgtatt
     3841 tccgcctgtc ggtcttcccg atcaccatcc cgcccctgcg cgatcgccgc gaggacgtga
     3901 cgctcctcgc gcggcatttc gcggcctgct tcgcggccga gcagaagcgc cgcggaccgg
     3961 tgacgttcga ccccgccgcc ctcgacgcga tgcgcgagta ctcctggcc
//
LOCUS       DASRIQ010000146         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1117786, whole genome shotgun sequence.
ACCESSION   DASRIQ010000146 DASRIQ010000000
VERSION     DASRIQ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117786"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..985)
                     /locus_tag="VFD06_03565"
     CDS             complement(290..985)
                     /locus_tag="VFD06_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZN02647.1"
                     /translation="MKLGLVDWTAVRRDLDDLGVARLPTILSSAECDAAIALYGDETR
                     FRKRVVMERLRFGAGEYQYFAAPLPPLVAQLRAAAYGPLAVIANDWQRHLGRATSFPR
                     TLDGFLRRCRATGQRKPTPLMLRYEAGGYNRLHQDLYGDLAFPLQMTFLLSRHGADFE
                     GGEMLFLENRPREQSIGTAVALRRGEGVVFAVGDRPIRGAHGFRRATLRHGVSRITSG
                     RRFTLGIIFHDAK"
     gene            complement(1010..1741)
                     /locus_tag="VFD06_03570"
     CDS             complement(1010..1741)
                     /locus_tag="VFD06_03570"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZN02648.1"
                     /translation="MTSVNQGTLFDLEATLHDRLRPEPVEAEAVRRIARGALRRLEQI
                     ERIRAEFVVDASERGVVAVRRGGRAEAASRAARLHADRGREELHEYLDGRRSYFGVPV
                     DLSRLAPFQRRVLEGAMRVPFGETTSYSALAARIGCAGAARAVGTALGHNPVPFIVPC
                     HRVLRGDRTLGGYAFGLPMKTDLLALERSTPILEGCTSTRILCRVGCGALARSRPDRR
                     VAFASIEDARSVGYRPCRVCRPEAA"
     gene            complement(1738..>2081)
                     /locus_tag="VFD06_03575"
     CDS             complement(1738..>2081)
                     /locus_tag="VFD06_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN02649.1"
                     /translation="WLYRILTNVCLDEIRRRRARPVEDGVSGREPADRRAADRAAERL
                     DVARALQRVPNEARALLVLRYADGLSYGELARVRGITVNTVKSQLKRGKEILKAALVE
                     RPRRRHEGGRG"
BASE COUNT          294 a          771 c          741 g          275 t
ORIGIN      
        1 gggcgccgtg atctccggcg gccgtcggcc gggcggtgtc gcccgccgcc gcccatgtcg
       61 atgtcagagc cagtagtgcc gcgcccgcga atcctcgagc catttcgtcc tcctcaccgt
      121 gcggcgccga agcgccgcga cccgggccac cgcggggagt ctggcatgtc ccgggccgcc
      181 ggcccacgga tgattcggac agcgatcgga ccattccggc cacatggtcg aaacgcgacg
      241 atcgggcgga ttcggccatg ccgcgctggg cttgcggggc cgccccgggt cacttcgcgt
      301 cgtggaagat gatcccgagg gtgaagcggc ggccggacgt gatccgactg acgccgtggc
      361 gcagggtggc gcgccggaag ccgtgcgcgc cgcggattgg acgatcgccg accgcgaaga
      421 ccacgccctc cccccgtcgc agcgccaccg ccgtgccgat cgactgctcg cgtggccgat
      481 tctcgaggaa gagcatctct ccaccctcga agtcggcgcc gtgtcggctc aggaggaagg
      541 tcatctgcag cgggaaggcg aggtcgccgt agagatcctg gtgcaggcgg ttgtacccgc
      601 ccgcctcgta ccgaagcatg agcggcgtcg gcttcctctg tcccgtcgcg cggcagcggc
      661 gcaggaaacc gtcgagagtg cgcgggaagg aggtcgcgcg cccgagatgc cgctgccagt
      721 cgttggcgat gacggcgagc ggtccgtagg cggcggcgcg cagctgcgcc acgagcggcg
      781 gcagcggcgc ggcgaagtac tggtattccc cggcgccgaa gcgcagccgc tccatcacca
      841 cgcgcttgcg gaagcgtgtt tcgtccccgt acagggcgat cgcggcgtcg cactcggcgg
      901 aagagaggat cgttggcagg cgcgcgacgc cgagatcgtc caggtcgcga cgcaccgccg
      961 tccagtcgac cagcccgagc ttcaagacgc cagactcccg cagcccaggt tatgccgcct
     1021 ccgggcggca gacccggcag gggcggtagc cgaccgagcg ggcgtcctcg atcgacgcga
     1081 aggcgacgcg gcgatcgggc cgcgaacggg ccagcgcgcc gcagccgacc cggcagagga
     1141 tgcgcgtgct cgtgcacccc tcgaggatcg gcgtggagcg ctccagggcc aaaaggtcgg
     1201 tcttcatcgg caggccgaag gcgtagccgc cgagggtccg gtccccgcgc agcacccggt
     1261 ggcaggggac gatgaacggc accgggttgt gcccgagcgc cgtccccacc gcgcgggccg
     1321 cgccggcaca gccgatgcgc gccgccaggg cggaatacga ggtcgtctcg ccgaacggga
     1381 cccgcatcgc cccttcgagg acgcggcgct ggaacggcgc gaggcgggag agatcgaccg
     1441 gcaccccgaa gtacgaacgg cggccgtcga gatactcgtg cagctcctcg cgcccccgat
     1501 ccgcgtgcag gcgcgccgcc cggctggcgg cttcggcgcg gccgccgcgg cgcaccgcga
     1561 ccacgccgcg ctcgctcgcg tccacgacga actcggcccg gatcctctcg atctgctcca
     1621 gccggcggag cgccccccgg gcgatgcgcc gcaccgcctc ggcttccacc ggttccggcc
     1681 gcaggcgatc gtgcagcgtc gcctcgagat cgaacagcgt tccctggttc acggacgtca
     1741 tcctcttcct ccttcatgtc ttcgtcgcgg ccgctcgacg agggccgcct tgagaatctc
     1801 cttgccgcgc ttcagctgac tcttcacggt gttcacggtg atgccgcgca cccgcgccag
     1861 ctcgccgtac gacagcccgt cggcgtagcg cagaacgaga agcgcgcgcg cttcgttcgg
     1921 cacccgctgc agcgcccgcg ccacgtcgag ccgctccgcc gcgcggtcgg cggcgcgccg
     1981 atccgccggc tcgcggccgc tcactccgtc ttccaccggc cgggcgcggc gccgccggat
     2041 ttcgtccagg cagacgttcg tcaggatgcg atacagccag g
//
LOCUS       DASRIQ010000147         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2674761, whole genome shotgun sequence.
ACCESSION   DASRIQ010000147 DASRIQ010000000
VERSION     DASRIQ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2674761"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFD06_03580"
     CDS             complement(<1..191)
                     /locus_tag="VFD06_03580"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491284.1"
                     /note="cleaves the ring of
                     2,3-dihydro-2,3-dihydroxybenzoyl-CoA forming
                     6-hydroxy-3-hexenoyl-CoA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA-dihydrodiol lyase"
                     /protein_id="HZN02650.1"
                     /translation="MSETATAAGRATPLVFETEPRRYKHWRLEVDGATARLVWDVKEG
                     EPLRPGYALKLNSYDLGVD"
     gene            288..1130
                     /locus_tag="VFD06_03585"
     CDS             288..1130
                     /locus_tag="VFD06_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN02651.1"
                     /translation="MPRRRATPEKPEGDSRLLLTHVGEAVRAARSARGMTRRAVAVRS
                     GVSERFLAQIESGIGNVSLLRLADVAAALEVSLVSLLAGPGPSSAPGRPAAAPRIALV
                     GLRGAGKSTIGRRLAKSLDAPFVELDARIEKAAGLTIGQIFELNGDRYFRRLEREVLE
                     KLAAAPGPMVLATGGGIVTEPETWALLRSAFTTVWLSATPEDHWTRVVAQGDRRPMAD
                     NPDAKAELKALLDARRPLYALADATIDTSALGPDRAARHILARIRDGWPAPAGAARRR
                     RSSS"
     gene            1082..1843
                     /locus_tag="VFD06_03590"
     CDS             1082..1843
                     /locus_tag="VFD06_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02652.1"
                     /translation="MARARRRGAPPPFLFVNPDIYRFLESASGILVAWSCLFTAITSI
                     RALRRWRQHAGPAPDSAVYTELAGLPLTILQPICFVWAAVTADWRSILLFVWWGPGFV
                     IAAVAVVLAKRRGRAIDWYPYRYAISILCKGYYLAYMAVFLRRSMYGMIFAFSVWIIN
                     DQYEKAFLSLDADRVRRTFDDRWLFRIAYPAGLLVPWFAQDMPHRTFALLYGTTLLFL
                     WLAGIHRVARAGLLRRRPADPSLLRNMVYFPRLRR"
     gene            1840..>2476
                     /locus_tag="VFD06_03595"
     CDS             1840..>2476
                     /locus_tag="VFD06_03595"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HZN02653.1"
                     /translation="MTTASPWVRDAVRFPLAFAQVREDPLLDRRVVELCGGRARVLMV
                     ASGGCTAAALAAWGGVSELHLVDPNPSQMALARLKLDLLGEAPADRRRLMGHEALPSQ
                     ARRHALEMRLLSASLPLDIIGPPDDVARLGPDHAGRYERLFAAFREALADSREALEEL
                     LRQDDPEEQSRRVEPGTTLGSALDAAFDDAFALPNLVALFGQEATRNPLEPF"
BASE COUNT          322 a          914 c          810 g          430 t
ORIGIN      
        1 atgtccacgc cgaggtcgta cgagttcagc ttcagggcgt agcccgggcg cagcggctcc
       61 ccttccttca cgtcccagac gagccgggcg gtcgccccgt ccacctcgag ccgccagtgc
      121 ttgtagcgcc gcggctcggt ctcgaagacc agcggtgtcg ccctccccgc ggcggtcgca
      181 gtttcactca cgttcgctcc ttcgtcgaag ccatcccccc gggggatgag cagtatattt
      241 cctgtttttt cgcatctgcc acgattgacg tgcactatac taccgcaatg ccccggcggc
      301 gcgccacacc cgaaaagccc gaaggggatt cccgcctcct gctcactcat gtgggggagg
      361 cggtgcgcgc cgcccgcagc gccaggggga tgacccgccg cgccgtcgcc gtccgatccg
      421 gggtctccga gcgcttcctg gcgcagatcg agtcggggat cggcaacgtg tcgctgctcc
      481 gcctggcgga cgtggctgcg gcgctcgaag tctccctcgt ctcgctgctg gccggccccg
      541 gaccttcatc ggccccggga cgccccgccg cggcgccgcg gatcgccctc gtcggcctgc
      601 gcggcgccgg caagagcacc atcggccgcc gcctcgcgaa atccctcgac gccccgttcg
      661 tcgagctgga cgcgcgcatc gagaaggccg ccggcctgac catcggacag atcttcgagc
      721 tcaatggcga tcgctacttc cgccgcctcg agcgcgaggt cctggagaag ctggcggccg
      781 cccccggccc gatggtgctc gcgaccggcg gcgggatcgt gacggagccc gagacctggg
      841 cgcttctgcg ctcggcattc acgaccgtgt ggctctccgc cacgcccgag gatcactgga
      901 cgcgcgtggt ggcgcagggg gaccgtcggc cgatggccga caacccggac gccaaggcag
      961 agctcaaggc cctgctcgac gcacgccgac cgctttacgc gctcgccgat gcgaccatcg
     1021 acacctcggc cctcggcccc gatcgggcgg cgcgtcacat cctcgcccgg atccgggacg
     1081 gatggcccgc gcccgccggc gcggcgcgcc gccgccgttc ctcttcgtga atcccgacat
     1141 ctaccgcttt ctcgagtcgg cgtcggggat cctggtcgcc tggtcctgcc tgttcacggc
     1201 gatcacctcg attcgagcgc tccgccgatg gagacagcat gcgggtccgg cccccgacag
     1261 cgccgtctac acggagctcg ccggactgcc gctgacgatc cttcagccga tctgcttcgt
     1321 ctgggccgcc gtcaccgcgg actggcgttc catcctgctt ttcgtgtggt ggggacccgg
     1381 cttcgtcatc gcagccgtgg cggtcgttct cgcgaagcgt cgaggccgcg ccatcgactg
     1441 gtatccgtac cgatacgcca tctctattct ctgcaaggga tactatctgg cgtacatggc
     1501 cgttttcctg cggcgctcca tgtacggaat gatcttcgcg ttcagcgtgt ggatcatcaa
     1561 cgaccagtac gagaaggcgt tcctgtccct ggatgccgat cgggtgcggc gcaccttcga
     1621 tgaccgctgg ctgtttcgaa tcgcctaccc cgccggcctt ctcgtcccgt ggttcgcgca
     1681 ggacatgccg caccgcacgt tcgccctgct gtacggcacg accttgctgt tcctctggct
     1741 cgcaggcatc caccgcgtcg ccagggcagg gctcctgcgc cgccggccgg cggacccgtc
     1801 gctgctccgg aacatggtgt acttcccgcg cctgcgacga tgacgacggc ctccccctgg
     1861 gtgcgcgatg cggtccgctt tccgctcgcc ttcgcgcagg tccgggaaga cccgcttctc
     1921 gatcgccggg ttgtcgaact ctgcggaggc cgggcgcgtg tcctcatggt cgcctccggc
     1981 ggatgcaccg ccgcggcgct ggcggcctgg ggcggagttt cggaattgca cctcgtcgat
     2041 ccgaatccct cccagatggc gctcgcgcgc ctcaagctgg atctcctggg ggaagcgccc
     2101 gcggatcggc ggcggttgat ggggcacgag gcgctcccgt cgcaagcgcg gcggcacgcg
     2161 ctcgagatgc ggctcctgag tgcatcgctg ccgctggaca tcatcggccc cccggacgat
     2221 gtcgcgcgac tcgggcccga ccacgccggc cgatacgagc gactgttcgc cgcgttccgt
     2281 gaggcgcttg cggacagccg cgaagccctc gaggagcttc tccggcagga cgatccggaa
     2341 gagcaatcgc gccgggtcga gcccggcacg acgctcgggt cggccctgga cgcggcgttc
     2401 gacgatgcct tcgccctgcc gaatctggtg gcgctgttcg gtcaggaggc gacgcgcaat
     2461 ccactcgagc cgttcg
//
LOCUS       DASRIQ010000148         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3178184, whole genome shotgun sequence.
ACCESSION   DASRIQ010000148 DASRIQ010000000
VERSION     DASRIQ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3178184"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VFD06_03600"
     CDS             <1..1032
                     /locus_tag="VFD06_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02654.1"
                     /translation="QPSNVGVWALPFSLARHEATGPPFLVAASGDVPSVSHDGTLLYG
                     SGALSLQTQMVWVDRTGKEIGPIGPVEEQWPFPEISPDGRHVAISARENDVEDVWISD
                     VERNTRTRMSAGVVTYSAQAWSPDGRTLIFGEGVSAPPALKRRASDGSGEAIAMGPGW
                     SPAYSADGRTIVYADVGKDTLWDLWSRPADGTSKPVPFYVVPGTQIWPRLSPDGRFIA
                     YVSDEGGTYDIYVKRFPSGEGKWQVSSGRGMWPRWGKSGRRLYYVNRDDLMEVDVATA
                     PDLHLGAPRRLFTRPSIRRALIFGWDPGYDVTADEQRFVFCRPVGKEEKNTGMIVVQN
                     WASEFAGGK"
     gene            complement(1042..>2951)
                     /locus_tag="VFD06_03605"
     CDS             complement(1042..>2951)
                     /locus_tag="VFD06_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02655.1"
                     /translation="GDLKRELQWIAEGGAAAAGSESSLAGASAARRPGARVHFAWAAA
                     LLLSVTVAAAAAIALLRPAPVTGDAPLLRASIAPPAGARFNIIGDVAGPPVLSPDGRR
                     MVFAAIDTGGNRLWVRSLEDLEARVLPGTDGAMYPFWSPDGRSVAFFTVDKVKRIDVD
                     GGAVYTICDAVSPRGGAWGPDGTIIFPSTFDKPLSRVPAGGGTPEPLTRLETGKHTTH
                     RWPQFLPDGRHFIFLAAHHEKQDSEDNGIYLGSLDGGEPVRLLASKSSAAYADGYLFY
                     VQGNALVTQRFDAAAGRTAGPAVPTAERVQLDRTTWRGIFSIGASTRSLVYMPAAGNF
                     GTRIVSYDITTGAVEAVSDEASQLNMNLTPDGRRLTVESQDGPRSDIWIYDLVDGSRS
                     RLTFDPMDESFATWVGRGDRIVFGSNRPDGRYRIYEKPSAGGTETLLFEWDQDVYPAD
                     VSADDRFLLINKGPFASRQATDLYVLDLRGTKTPIPFATAPYEETDGQFSPDGRWIAY
                     TSTQSGQEEIYVAPRPDPATASAGGAASRGQWQVSSGGGSVPRWGSAGRTIFWRRADN
                     TTFARTEVDGRGVTFIVGPTRDLPFRAYQNWSSLSYDVSADGRRLVVASPGGDSAQTM
                     ALVSDWSAIVK"
BASE COUNT          446 a         1032 c         1053 g          420 t
ORIGIN      
        1 cagccatcca acgtcggcgt ctgggcgctg ccgttctcgc tggcccggca cgaggcgacc
       61 ggcccgccgt tcctggtcgc cgcgtccggc gacgtcccga gcgtcagcca cgacggcacc
      121 ttgttgtacg gcagcggggc gctctccctc cagacgcaga tggtctgggt ggatcgcacc
      181 ggcaaggaga tcgggccgat cggcccagtt gaggagcagt ggcccttccc ggagatctct
      241 cccgacggcc gccacgtcgc catcagcgcg cgggagaacg acgtcgagga cgtctggatc
      301 tccgacgtcg agcggaacac gcgcacccgg atgtccgccg gcgtcgtcac ttactcggcg
      361 caggcctggt ccccggacgg gcgcacgctg atcttcggcg agggggtttc cgcgccgccg
      421 gccctcaagc ggagggcctc ggatggcagc ggcgaggcga tcgccatggg ccccggatgg
      481 tcaccggcgt acagcgccga cggccggacc atcgtctacg ccgacgtcgg gaaggacacg
      541 ctctgggacc tctggtcgcg ccccgccgac ggcacctcca agccggtccc gttctacgtg
      601 gtgcccggca cgcagatctg gccgcgcctc tcccccgacg gccgcttcat cgcttacgtt
      661 tccgacgagg ggggaaccta cgacatctac gtcaagcgct tcccgtccgg ggaagggaag
      721 tggcaggtgt cgagcggccg cggcatgtgg ccgcgctggg ggaagagcgg ccggcgcctc
      781 tactacgtca accgggatga cctgatggag gtggatgtcg ccaccgcccc cgacctgcac
      841 ctcggggcgc cgcgccggct gttcacgcgc ccctcgatcc gccgcgccct gatcttcggc
      901 tgggacccgg gctacgacgt caccgcggac gagcagcgct tcgtcttctg ccggccggtc
      961 ggcaaggagg agaagaacac cgggatgatc gtggtgcaga actgggccag cgagttcgcc
     1021 ggggggaagt gaaggaagcg gctacttcac gattgccgac cagtcggaga ccagcgccat
     1081 cgtctgggcg ctgtctccgc cgggggaggc caccacgagc cggcggccgt cggcgctgac
     1141 gtcgtaggag agcgacgacc agttctggta ggcgcggaac gggagatcgc gcgtcggtcc
     1201 aacgatgaac gtcacgcccc gcccgtccac ttcggtccgg gcgaaggtcg tgttgtcggc
     1261 ccggcgccag aagatcgtcc gcccggcgct cccccatcgc ggcacgctgc ctccccccga
     1321 cgacacctgc cactggccgc gcgacgcggc gcctcccgcg gaggccgtcg cgggatcagg
     1381 gcgaggagcg acgtagatct cctcctgccc cgactgcgtc gaggtgtagg cgatccatcg
     1441 accgtcggga gagaactggc cgtcggtctc ttcgtaaggc gcggtcgcga acgggatcgg
     1501 tgtcttcgtt ccccggagat ccagcacata gagatcggtc gcctgccgcg acgcgaaggg
     1561 tcccttgttg atgagcagaa accgatcgtc ggccgagaca tcggcgggat acacatcctg
     1621 gtcccattcg aacagcagcg tctcggtgcc gccggccgac ggcttctcgt agatgcggta
     1681 gcggccgtcg ggccggttcg acccgaagac gatgcggtcc cccctgccga cccacgtcgc
     1741 gaacgactcg tccatcggat cgaaggtgag ccggctgcgc gagccatcga cgaggtcgta
     1801 gatccagatg tctgatcgcg gaccgtcctg cgactccacc gtgagccgcc ggccgtcggg
     1861 ggtcaggttc atgttcaact ggctcgcctc gtcggacacg gcctcgacgg cgccggtggt
     1921 gatgtcgtag gacacgatgc gggtcccgaa attcccggcc gccggcatgt agacgagcga
     1981 gcgggtggag gcgccgatcg agaagatgcc gcgccacgtc gtccggtcga gctggacccg
     2041 ctccgccgtc gggacggccg ggcccgcggt gcgccccgcg gcggcgtcga aacgctgcgt
     2101 caccagggcg ttcccctgca cgtagaacag gtatccgtcg gcgtacgcgg cgctgctctt
     2161 ggaggcaagg agccggacgg gttcgccgcc gtcgagcgag cccagataga tgccgttgtc
     2221 ctcggagtcc tgcttctcgt gatgcgccgc caggaagatg aaatggcggc cgtcgggcag
     2281 gaactgcggc cagcggtgcg tcgtgtgctt cccggtctcg agacgggtca gcggctcggg
     2341 cgtccctccc ccggccggaa cccgggagag cggcttgtcg aaggtcgacg gaaagatgat
     2401 cgtcccgtcc ggcccccaag cgccgccgcg cggggacacc gcgtcgcaga tggtgtacac
     2461 ggccccgccg tcgacgtcga tccgcttcac cttgtcgacg gtgaagaagg cgacgctccg
     2521 gccgtccggc gaccagaacg ggtacatggc cccgtcggtc cccgggagca cacgggcctc
     2581 gaggtcctcg agagaccgga cccagagccg gttcccgccg gtgtcgatcg ccgcgaacac
     2641 catgcggcgg ccgtccggcg acaggaccgg cgggccggcg acgtccccga tgatgttgaa
     2701 ccgcgctccc gcgggcggtg cgatggaggc gcgcagaagc ggtgcatcgc cggtgaccgg
     2761 ggcaggacgg aggagcgcga tcgctgcggc ggccgcgacg gtcaccgaca acagcagcgc
     2821 ggccgcccac gcgaaatgga cccgggcgcc cggccgccgc gccgccgagg cgccggcgag
     2881 gctcgactcg gaaccagccg cggcggcacc gccttctgcg atccactgca gctcgcgctt
     2941 gaggtcgccg g
//
LOCUS       DASRIQ010000149         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_832918, whole genome shotgun sequence.
ACCESSION   DASRIQ010000149 DASRIQ010000000
VERSION     DASRIQ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832918"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFD06_03610"
     CDS             <1..612
                     /locus_tag="VFD06_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN02656.1"
                     /translation="LEIFPGESIAIIGPSGSGKSTLMHIIGCLDVPSSGDYHLAGTPV
                     ATLGSRALAKVRNQRIGFVFQSYNLLPKASLLRNVELPMLYGGVGRQERRERAREALR
                     KVGLLERASNLPSQLSGGQRQRVAIARALVNNPSIVLADEPTGNLDTQTGKDILALFD
                     ELGRQGHTIILVTHDPSVAERASRIVRIVDGQIAADGAAVVAH"
     gene            623..1855
                     /locus_tag="VFD06_03615"
     CDS             623..1855
                     /locus_tag="VFD06_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02657.1"
                     /translation="MTLRESILDALVEVRVNRARTVLQTLGVILGVASLVAVQGMSDA
                     GRRQSVKFFSEFGGLRKILVLNKPVKERVQTATMRASHGLDWGDVAALRREVPQATQV
                     DPIAEEMLNIRTPTYRKLREVTGATPDYQAVYKFFPARGRFLIDDDLASMSRVVVLGD
                     SAARLYFGNEDPLGKTLYIGDGGFRVVGVLRRKEFYFNEGDRNALEWMNRLTIIPLTA
                     LYSRFTGDPDKKVSYINVMVDKVENNQKAVGAIRKVLERRHKGVRDFEVIDRKERMKQ
                     QQQQGQIFDIIFMATGIVSLLVGGIVIMNIMMASLRERIREVGVRKAIGARGLDVALQ
                     FLVESVLVTSIGGLVGLPIGVLFANAITALIGQPAVITPQMALVSVIASVAVGLFFGL
                     YPAIKAARLNPVEALRYE"
     gene            1852..>2782
                     /locus_tag="VFD06_03620"
     CDS             1852..>2782
                     /locus_tag="VFD06_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02658.1"
                     /translation="MSLDGFGESVRTSLVEIWSHKLRSTLTLLGIILGTLSITVMTSL
                     LDGVTTAVWDGFQDLGFDGVVYVMTRPPRDLREQAVFARSRGLQPEDADVLLARGRAV
                     NSVAPAIVTETMVRRGSLERRVRTMGVTSTYNDVRSRSVETGRFFSVLEEGSFAPVAV
                     LGHRLNKRLFGAEDPVGKWIQVDGRAFKVIGVGEKLGNSFINDDDFVEEMEGIYVPLA
                     TLRAYYSGGDAPLSFMAIKTDNPDRLSDLRSEAEASLVLAHHGANDFRVENIAQEILK
                     VRKEIKVVLRNWHIVLGTIAGISLLVGGIGLLSV"
BASE COUNT          434 a          957 c          964 g          427 t
ORIGIN      
        1 ctggagatct tccccggcga gtcgatcgcc atcatcggac cgtccgggtc gggaaaatcg
       61 accctgatgc acatcatcgg ctgcctcgac gtcccctcga gcggcgacta ccacctcgcc
      121 ggcacgccgg tggcgaccct cgggtcgcgc gctctggcga aggtgcgcaa ccagcggatc
      181 ggcttcgtgt tccagtcgta caacctgctg ccgaaggcga gcctgctgcg caacgtcgag
      241 ctgccgatgc tgtacggcgg ggtcgggcgg caggagcgcc gcgagcgggc gcgcgaggcg
      301 ctccggaaag tcggcctgct cgagcgcgcc tcgaacctgc cgtcgcagct ctccggcgga
      361 cagcggcagc gcgtggcgat cgcccgggcg ctcgtcaaca accccagcat cgtgctggcc
      421 gacgagccga ccgggaacct cgacacccag accggcaagg acatcctcgc gctgttcgac
      481 gagctggggc ggcaggggca cacgatcatc ctcgtgaccc acgacccgag cgtcgccgag
      541 cgcgcctccc ggatcgtccg gatcgtcgac gggcagatcg ccgccgacgg cgcggcggtc
      601 gtcgcccact gagggccgcg ccgtgaccct gcgcgaatcg atcctcgacg ccctggtgga
      661 ggtgcgggtg aaccgggccc gcaccgtgct gcagacgctc ggggtgatcc tcggcgtggc
      721 gtcgctcgtg gcggtgcagg ggatgagcga cgccggccgc cgccagtcgg tgaagttctt
      781 ctcggagttc ggcggcctcc gcaagatcct cgtcctcaac aagccggtga aggagcgggt
      841 gcagaccgcc accatgcgcg ccagccacgg cctcgactgg ggggacgtcg cggcgctgcg
      901 ccgcgaggtg ccgcaggcga cccaggtcga cccgatcgcc gaggagatgc tgaacatccg
      961 caccccgacc taccggaagc tgcgcgaggt gaccggcgcc acccccgact accaggcggt
     1021 ctacaagttc ttcccggcgc gcggccgctt cctcatcgac gacgacctgg cctcgatgtc
     1081 gcgcgtcgtc gtcctcggcg actcggcggc gcgcctctac ttcggcaacg aggatccgct
     1141 gggcaagacg ctgtacatcg gcgacggcgg cttccgcgtc gtgggagtgc tgcggcgcaa
     1201 ggagttctac ttcaacgagg gggaccggaa cgccctcgag tggatgaacc gcctgaccat
     1261 catcccgctc accgcgctgt actcgcgctt caccggcgac cccgacaaga aggtctccta
     1321 catcaacgtc atggtcgaca aggtggagaa caaccagaag gcggtcgggg cgatccgcaa
     1381 ggtgctggag cggcgccaca agggggtccg cgacttcgag gtgatcgacc gcaaggagcg
     1441 gatgaagcaa cagcagcagc aggggcagat cttcgacatc atcttcatgg ccaccggcat
     1501 cgtgtcgctc ctggtcgggg ggatcgtcat catgaacatc atgatggcgt cgctgcgcga
     1561 gcgcatccgc gaggtcgggg tgcgcaaggc gatcggcgcc cgcgggctcg acgtggcgct
     1621 gcagttcctc gtcgagtcgg tgctggtgac gtcgatcggc ggcctcgtcg gcctgccgat
     1681 cggcgtgctg ttcgccaacg ccatcaccgc cctcatcggc cagccggcgg tgatcacgcc
     1741 tcagatggcg ctggtgagcg tcatcgcctc ggtggcggtc gggctgttct tcggcctgta
     1801 cccggcgatc aaggcggccc ggctcaaccc ggtcgaggcg ctgaggtacg aatgagcctc
     1861 gacggcttcg gcgagagcgt ccgcacctcg ctcgtggaga tctggagcca caagctgcgc
     1921 tccaccctga cgctgctcgg catcatcctg gggacgttgt cgatcaccgt catgacgtcg
     1981 ctgctcgacg gggtgaccac cgccgtctgg gacggcttcc aggatctcgg attcgacggc
     2041 gtcgtgtacg tcatgacgcg gccgccgcgc gacctgcgcg agcaggccgt gttcgcccgc
     2101 tcgcgcggcc tgcagccgga ggacgccgac gtcctcctgg cgcgcggccg ggcggtgaac
     2161 tcggtggctc cggcgatcgt caccgagacg atggtgcgcc gcggcagcct cgagcggcgc
     2221 gtgcggacca tgggggtcac gtccacctac aacgacgtcc gcagccgcag cgtcgagacc
     2281 gggcgcttct tcagcgtcct cgaggaaggc tcgttcgccc cggtggccgt cctcgggcac
     2341 cggctgaaca agcgcctgtt cggcgccgag gacccggtcg gcaagtggat ccaggtggac
     2401 ggccgcgcct tcaaggtgat cggcgtcggc gagaagctcg gcaactcctt catcaacgac
     2461 gacgacttcg tcgaggagat ggaggggatc tacgtgcccc tcgcgacgct gcgcgcctac
     2521 tactcggggg gcgacgcgcc cctctcgttc atggcgatca agaccgacaa tccggaccgt
     2581 ctcagcgatc tgcgctcgga ggcggaggcc tcgctcgtgc tggcgcacca cggcgcgaac
     2641 gacttccggg tggagaacat cgcccaggag atcctgaagg tccgcaagga gatcaaggtc
     2701 gtcctgcgca actggcacat cgtgctgggg acgatcgccg ggatctcgct cctcgtcggc
     2761 ggcatcggcc tgctctcggt ga
//
LOCUS       DASRIQ010000150         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2934725, whole genome shotgun sequence.
ACCESSION   DASRIQ010000150 DASRIQ010000000
VERSION     DASRIQ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2934725"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..685)
                     /locus_tag="VFD06_03625"
     CDS             complement(38..685)
                     /locus_tag="VFD06_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZN02659.1"
                     /translation="MKTTALLLAAGESARMGTPKALLLWGGRPLLGHQLAVLQRSRVD
                     ECIVVLGREAGRLGGLVQPLLRPGWKARAVVNPRPADGKSSSIRAGLAALSRPPESIL
                     IAAIDQPLERDLVDALLEAGRREWELPGDGWPIKRIVIPTFDGHRGHPPLFHGSLFPE
                     LLGVSEETEGLRRVVRRIPERVLEVPWLRSGILLNLNTRSDIESTRGPGSSYRSA"
     gene            complement(682..1803)
                     /locus_tag="VFD06_03630"
     CDS             complement(682..1803)
                     /locus_tag="VFD06_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZN02660.1"
                     /translation="MFAKEGLQGVVAARTGLSRIDGERGVLAYRGYSIDDLARHASFE
                     EVVHLLWHGELPNRAALDETRRALAQQRLLSPGVAELVQGFARDGGPMDVLRTAVSAM
                     AIGDPDLHESTREANLRKGCRIAAQMPTVIALHDRARKGLEPVRPDPELGHAANFLWM
                     LTGRRPEELAVRCLDVALILQAEHGLNASTFAARVAAGTLADLHAALTAAIAALKGPL
                     HGGANHDVMVMLRTIGDPAAAADYVRAALAQKRKIPGFGHRIYRTLDPRAVHLKELSA
                     RLAQATGQETWYRMSTVIEATCKEAKNLNANVDFYAASTYSMLGFDMDLFTPIFAASR
                     SPGWIAHVIEQHTDNRLIRPLEEYIGPPDRDWVPLEERA"
     gene            1876..2907
                     /locus_tag="VFD06_03635"
     CDS             1876..2907
                     /locus_tag="VFD06_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HZN02661.1"
                     /translation="MAAVEEGLGPDDLRLARQRVARGARVTPLLPSDLLTERLGQAVA
                     LKCENLQAGGAFKIRGATNFVASLDDEARARGLITYSSGNHAIALSLAARRAGVPAVV
                     VMPETAPAIKRERARALGAELHFCGTTSLERHARAKALAEERGLTMVPPFDHPAIVAG
                     QSTCGVEIIEQLPEAAAVIVPVGGGGLLAGIALACAYHKRGVRVIGVEPKGAPKMSSS
                     LAARAPVTLASVGSIADGLLPSRPGDITFAVAQRYVHEVVTVEEGAIADAARRLLVDE
                     RLLVEWSGAVGVAALLSGALRLPGGPLAIVLSGGNVDPAAILSCGTSGGPAAPPSGPA
                     VTPPSPVFA"
     gene            2987..3454
                     /locus_tag="VFD06_03640"
     CDS             2987..3454
                     /locus_tag="VFD06_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HZN02662.1"
                     /translation="MDRTRHRAERGFTLIELLIVVTIIGIIAALAFPNLRNAMDRAKQ
                     KATMTNMRGIGNALEMYSVDFNTYPRGLNDAGASTLSGMLSPNYLRTVPPADEWSGAW
                     HVDTNTSGSQYTITSYGRDGQPGTNTGGPTGNVDCDIVYTNSAFFQWPSNQQQ"
     gene            3607..3801
                     /locus_tag="VFD06_03645"
     CDS             3607..3801
                     /locus_tag="VFD06_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02663.1"
                     /translation="MATRTQEKDGRDQESVLPPGQRDRTGFWYPAGYEDPATMVRRRP
                     RRHAEIAGEAESSEDLTEPR"
BASE COUNT          561 a         1415 c         1366 g          542 t
ORIGIN      
        1 cgcctgttcg aatggagcgg cgagcgttcg atggtcttca cgcagaacgg taagaactcc
       61 ctggcccgcg tgtcgattca atatcgcttc gggtattgag gttcaggagg atcccggagc
      121 gcagccacgg cacctcgagg acgcgctccg ggatccgccg caccacgcgg cggagcccct
      181 cggtctcctc gctcacgccc agaagctccg ggaagaggga gccgtggaac agcggcgggt
      241 ggccgcgatg gccgtcgaag gtcgggatca cgatccgctt gatgggccag ccgtcaccgg
      301 ggagctccca ctcccgcctc cccgcctcga gcagcgcgtc gacgagatcc cgctcgagcg
      361 gctgatcgat cgcggcgatc aggatcgact cgggcggcct cgacagcgcc gcgaggccgg
      421 cccggatcga cgacgacttg ccgtcggcgg ggcgcgggtt gacgacggcg cgcgccttcc
      481 agccgggacg caggagaggc tgcacgagcc ctccgagccg acccgcctcg cgtcccagca
      541 cgacgatgca ctcgtccacg cggctgcgct gcaggacggc gagctggtgg ccgagcagcg
      601 gccttccgcc ccataggaga agtgccttcg gcgttcccat gcgggccgac tctccggccg
      661 ccagcagcag tgccgtggtc ttcatgcgcg ttcctccagg ggaacccagt cccggtcggg
      721 cggcccgatg tactcctcga gtggacggat cagccggttg tcggtgtgct gctcgatgac
      781 gtgcgcgatc cagccgggcg aacggctggc ggcgaagatc ggcgtgaaca gatccatgtc
      841 gaaccccagc atcgaatagg tcgaggcggc gtagaagtcg acgttggcgt tgaggttttt
      901 cgcctccttg caggtcgcct cgatcaccgt cgacatgcgg taccaggtct cctggccggt
      961 cgcctgcgcc aggcgggcgg acagctcctt gaggtggacg gcacgcgggt cgagggtgcg
     1021 atagatccgg tgcccgaacc cggggatctt gcgcttctgc gccaacgcgg cgcgcacgta
     1081 atcggcggcg gcggccggat ccccgatggt ccgaagcatc accatgacgt cgtggttggc
     1141 gccgccgtgc agcggcccct tcaacgccgc gatcgccgcc gtcagcgcgg cgtgcaggtc
     1201 ggcgagggtg ccggccgcga cccgcgccgc gaaggtggag gcgttcaggc cgtgctccgc
     1261 ttggaggatt aacgcgacgt cgagacagcg gaccgcgagc tcctccggac gccgcccggt
     1321 cagcatccac aggaagttgg cggcgtgccc gagctcgggg tcggggcgca cgggctcaag
     1381 acccttgcgg gcgcggtcgt gcagcgcgat gaccgtcggc atctgcgccg cgatccggca
     1441 ccccttgcgc aggttcgcct cgcgcgtcga ctcgtgcagg tcgggatcgc cgatcgccat
     1501 cgccgacacg gcggtgcgca gcacgtccat cggcccgccg tcccgcgcga atccctggac
     1561 cagctccgcg acgcccggag agaggagacg ctgctgcgcc agggcgcggc gggtctcgtc
     1621 gagcgcggcg cggttcggca gctcgccgtg ccacaggagg tgcaccacct cctcgaagga
     1681 ggcatgccgg gccaggtcgt cgatcgagta gccgcggtag gcgagaaccc cgcgctcgcc
     1741 gtcgatgcgg ctcagcccgg tgcgggcggc gacgaccccc tggaggcctt ccttggcgaa
     1801 catcgatcgg ctccgccccc cgctgcgatc cggcgggtca ttataatgaa gccccccgga
     1861 gggagacagg gagacatggc ggccgtcgaa gaagggctgg gaccggacga tctgcgcctg
     1921 gcgcgccagc gggtggcgcg cggcgcccgc gtcaccccgc tcctcccctc cgacctgctg
     1981 accgagcggc tcggccaggc ggtcgcgctc aagtgcgaga acctgcaggc gggcggggcg
     2041 ttcaagatcc gcggggcgac caacttcgtc gcctcgctcg acgacgaggc gcgcgcccgc
     2101 ggcctcatca cctactcctc cgggaaccac gccatcgccc tgtcgctcgc ggcgcggcgc
     2161 gccggggtcc cggcggtcgt ggtgatgccg gagaccgcgc cggcgatcaa gcgcgagcgg
     2221 gcgcgggcgc tcggcgcgga gctccatttc tgcggcacca cgtcgctcga acgccacgcg
     2281 cgcgccaaag cgctcgcgga ggagcgcggc ctgaccatgg tcccgccgtt cgatcacccc
     2341 gccatcgtcg ccgggcaatc gacatgcggg gtcgagatca tcgagcagct cccggaggcg
     2401 gccgcggtga tcgtcccggt cggcggcggc ggccttctgg ccggcatcgc gctggcctgc
     2461 gcctaccaca agcgcggcgt gcgggtcatc ggcgtcgagc cgaagggggc gccgaagatg
     2521 tcctcgtcgc tcgccgcacg cgccccggtc accctcgcct ccgtcggctc gatcgccgac
     2581 gggctcctgc cgagccgtcc cggcgacatc accttcgcgg tggcgcagcg ctacgtgcac
     2641 gaggtggtga cggtcgagga gggggccatc gccgacgccg cacggcgcct gctggtcgac
     2701 gagcggctcc tggtcgaatg gagcggcgcc gtgggtgtcg ccgccctgct ctccggggcg
     2761 ctgcggctcc ccggggggcc gctggcgatc gtcctgagcg gggggaacgt cgatccggcc
     2821 gccatcctca gttgcggcac atcgggaggc cccgccgcgc cgccgtcggg cccggccgtc
     2881 acccccccct ctcccgtgtt cgcctagacg gttcctcgtc cctctccgta aggaggggaa
     2941 cagcgtgcta gactcgcgcg cttttcagag gagccgatcg atgtcgatgg accggacgcg
     3001 ccaccgcgcc gagcgcggct tcaccctcat cgagcttctg atcgtcgtca ccatcatcgg
     3061 catcatcgcg gcgctggcgt tccccaacct gcgcaacgcc atggatcgcg ccaagcagaa
     3121 ggcgaccatg accaacatgc gggggatcgg caacgcgctc gaaatgtact cggtcgactt
     3181 caacacctac ccgcgcggcc tgaacgacgc cggcgcgtcg acgctctccg gcatgctctc
     3241 ccccaactat ctgcgcaccg tcccgcccgc cgacgagtgg tccggagcct ggcacgtcga
     3301 caccaacacc agcggcagcc agtacaccat cacctcgtac ggccgcgacg gccagcccgg
     3361 caccaacacc ggcgggccga cgggcaacgt cgactgcgac atcgtctaca ccaacagcgc
     3421 cttcttccag tggccgtcca accagcagca gtaggtgcgc ggcgcccccg gcgtcgcgcc
     3481 cgcggtaaaa aacgacgtct tgccgtccgc caccgccctg cgctacgatg cgcttgctct
     3541 cgcacatcga catgtcgcag gatcggcgcc cgcgctccgg cgcgcgcgcg ggtccgggga
     3601 gggatcgtgg cgacccgcac ccaggagaag gacgggaggg atcaggaatc ggtgctcccc
     3661 cccggccagc gggatcgcac cgggttctgg tacccggccg ggtatgaaga cccggcgacg
     3721 atggtgcgcc ggcggccgcg ccgccacgcc gaaatcgccg gcgaggccga gtcctccgag
     3781 gatctgacgg aaccccgctg acgtgtcccc cacggggcct cctcctttcg cggtccatcc
     3841 ggagggggcg cgcgtcccgg ccgaggtgga gcggctggcg gcgc
//
LOCUS       DASRIQ010000151         9383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1624818, whole genome shotgun sequence.
ACCESSION   DASRIQ010000151 DASRIQ010000000
VERSION     DASRIQ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624818"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFD06_03650"
     CDS             complement(<1..619)
                     /locus_tag="VFD06_03650"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZN02664.1"
                     /translation="MSWRQNRSKAVGIIAAACLAAGAAWAAETQGTAGAPPAKKANDP
                     VKPSAPSAKPSGPAAPSAPRPDAPVAKVNGAPLLRRDFDLTVQMIFRKRGAGERSHAD
                     LDAVRQAALDIMIDNELLYQKAKEAGVTVTDAEAKEEAARLKAALGPPETAAAFLKEI
                     GATDADLVAQVRRTISIGRFVDQKVAGSIAVGEKEARAWYDAHPEA"
     gene            complement(594..1676)
                     /locus_tag="VFD06_03655"
     CDS             complement(594..1676)
                     /locus_tag="VFD06_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZN02665.1"
                     /translation="MSDEALRDRALAALRTVKDPELHVDLVTLGLVEGLRVDGSDVTL
                     TVNLTTPGCPLKGQIEKDVRTALGQVPGIGDVHLTMGAKVKRSIAAPAELIPEVANTV
                     AVGSGKGGVGKSTVAANLALSFARSGAAVGLMDADLYGPNLVQMLGVKERPEIREKRI
                     VPVTVHGLKLMSMAFFLKEDEPVVWRGPMLHGAIRQFLGDVEWGALDYLFIDMPPGTG
                     DVQLTLSQVIPLTGAVLVATPQDVALSDVRKAAAMFAKVNVPILGVVENMSYFECPHC
                     GERSEIFSHGGAARLAEKLGVPCLGSIPLDVRLRQGGDEGRPPVIYDPKSGPAILFKE
                     IAERLAAIISVQTWKGEAHELAAEPK"
     gene            complement(1673..2851)
                     /locus_tag="VFD06_03660"
     CDS             complement(1673..2851)
                     /locus_tag="VFD06_03660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZN02666.1"
                     /translation="MNAVAPAAAASLEPILVEAIRAAGPIPFARFMEICLYHPEHGYY
                     MRGLGGGGGRDYLTSSGVHRAYGTLVARQAAEMWRLLGTPARFRFVEFGPGEGRFARD
                     VLEASRSDRPFAAALEYVLVETSPALRERQRRLLAPGGEGAAGAPSVSWNTLEGLESE
                     ADERDGFAGCLFANEVLDAFPVHRVTGTPEGPREVHIDVENGVFREKLLPLSTPDLVE
                     DLDARGLAPAPGQIVDVAPAAAHFVGRAARLLSRGWFVLVDYGDRAEDLFHPARRRGT
                     LRAFHRHRLVDDVLARPGEQDLTAHVDFTAVRRAAATAGCEVAGQVSQSHFLLALGAL
                     EGFEAQDLAGREALKDLVLPDRMGGAFQVLVLARGAAPAGLRGLSAPWRDRPAGKGTA
                     "
     gene            complement(2848..3420)
                     /gene="nuoB"
                     /locus_tag="VFD06_03665"
     CDS             complement(2848..3420)
                     /gene="nuoB"
                     /locus_tag="VFD06_03665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HZN02667.1"
                     /translation="MSDIDRDAEPWLLTTRLADYIAWGRKNSLWPMPMGLACCAIEMM
                     AVVAPRYDIARFGAEAMRFSPRQADLMIVAGTVVKKMAPAVKRIYDQMPEPKWVIAMG
                     ACASTGGMFRSYAVTQGVDELIPVDVYIPGCPPRPEALIEGVMQIQRLIDARARAARG
                     AAPAAPVKDAAAPAAPIQGAAAPATAAAGK"
     gene            complement(3417..3992)
                     /locus_tag="VFD06_03670"
     CDS             complement(3417..3992)
                     /locus_tag="VFD06_03670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HZN02668.1"
                     /translation="MPAMPMTGKPVKRPSPKGLWARSYLPSVLKGMWITFRHLFRPKV
                     TMQYPEEKWAMPDRYRGLPALVRDEQERVKCVACFLCQYVCPPKAIHIEAQEIGTGIE
                     KGPKVFDINMLRCIMCGYCEEVCPEEAIFLTKEYQTVGTSREGLIYHKDRLLEMGGIR
                     KDTTHKWGPLEKPRPKAQVAATAPEAAGGTR"
     gene            complement(3974..4396)
                     /locus_tag="VFD06_03675"
     CDS             complement(3974..4396)
                     /locus_tag="VFD06_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HZN02669.1"
                     /translation="MTAGEGAGEYSATLLDHYRHPRNVGDIEGSAAVAQVGDPAHGDV
                     LRFGMRIEGDQVIEARFRSFGCTAAIAAGSMATVMVTGKRLEEVERVSNRDVADALGG
                     LPESKRHCSVLAEQAIREALRRWRDAAGGGEASCPPCR"
     gene            complement(4496..5557)
                     /gene="moaA"
                     /locus_tag="VFD06_03680"
     CDS             complement(4496..5557)
                     /gene="moaA"
                     /locus_tag="VFD06_03680"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZN02670.1"
                     /translation="MSPAAPGGPAAAMAPAPEPVSPTGPLVDRFGRAVRSLRISVTDR
                     CNLRCVYCMPLAGITWYERSELLTFEEIVRVVRPLAARGVREIRLTGGEPLVRRDLAR
                     LAGMLSALPGVEDLAVTTNGLLLKEQAEGLVRAGVRRFNVHIDSLDPETYRKVGRRDG
                     LGRVLAALDELERLGALPIKVNVVLIRGVNDQEIPRFADLARTRRCQVRFIELMPLGA
                     GEAFEASLIVPGAEVRARIEAGHPLRPAPRPRDASPAEVFRFEDGAGDVGFINSVTEP
                     FCDRCDRIRLTADGMVRNCLFSRGDRDLRPILRGGGSDDELVRAIAAEVDAKGPGGSL
                     DMTAVYRERLPRKMWQIGG"
     gene            complement(5563..5940)
                     /locus_tag="VFD06_03685"
     CDS             complement(5563..5940)
                     /locus_tag="VFD06_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZN02671.1"
                     /translation="MNLPYARHVLICVGPRCGGERGSGRIRQEFRKEFVRQQIDTTVK
                     DTECLCFGLCTYGPNVIIYPEGVVYSGVKPADVAEIVRRHVARGQTVDRLLYKKKEAP
                     PAPAAEDDDFDVFRDGDPVPPRG"
     gene            complement(5937..6401)
                     /locus_tag="VFD06_03690"
     CDS             complement(5937..6401)
                     /locus_tag="VFD06_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN02672.1"
                     /translation="MPGETEKSIESLGWKLPEVGKPTGNWVQTVRSGNLLFISGKFAK
                     EGGKLKYPGRIGREVTVEQGSDAARLAALSVLATAKQAIDDLDRIRRVVQLVGHIAIA
                     PGFSDAFTVMEGASDVFVKVFGDRGRHTRLAHGVDELPQNACLQLQAVLEVE"
     gene            6543..7346
                     /gene="kdsB"
                     /locus_tag="VFD06_03695"
     CDS             6543..7346
                     /gene="kdsB"
                     /locus_tag="VFD06_03695"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZN02673.1"
                     /translation="MRPPDDASTAADALGVVPARYGSTRFPGKPLVDIAGKPMIRHVW
                     ERASEARLLKGVVVATDDERIRRVVEDFGGDVMMTSPDHPSGTDRLAEVARSRPATLY
                     VNIQGDEPLVDPDDLDVLVSGMVAEPGFEMGTLSVPLDDPRAVEDPNVVKVVCDVAGR
                     ALYFSRAPIPYPRQPSQPTEGGPDPFVFRGSWRRHIGLYAYRSAFLRAFATWEPTPLE
                     RTEGLEQLRALERGSPIRVFPARGRYVAVDTPGDLRLVESALGAVLKGR"
     gene            7349..8959
                     /locus_tag="VFD06_03700"
     CDS             7349..8959
                     /locus_tag="VFD06_03700"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZN02674.1"
                     /translation="MPTKYIFVTGGVVSSLGKGLAAASIGRLLEARGFSVALQKFDPY
                     LNVDPGTMSPFQHGEVYVTEDGVESDLDLGHYERFTSTQTSRDHNFTSGKIYEHVIAK
                     ERRGDYLGGTVQVIPHVTDEIKESIRRISKGVDIQIVEIGGTIGDIESLPFLEAIRQF
                     RLEVGRSNAVFVHLTLVPYIGAAGELKTKPTQHSVRDLRAIGIQPDVLLCRTDRFIPP
                     EIKKKIALFCNVPEEAVITAKDVETIYEVPLVLAKEGVDAILLKDLDLPYRDKNMDDW
                     VEMVGKAKNPSDGEVVVGIVGKYVAYEDSYKSLNEALTHGGIANDARVRLHWIEADRL
                     VGGGMQEQMAPVHGILVPGGFGLRGVDGMIEAIRFARERRVPFFGICLGMQCAVIEYA
                     RNVAGLKDADSAEFTPETPHPVIYKLRELEGVERMGGNMRLGKWPCLVRTGSQAHKAY
                     ARTEISERHRHRYEVHRKYMDDLERRGLKVTGETPDKRFVEIVEIPDHPYFLACQFHP
                     EFKSKPMQPHPLFRSFIDACKRYRQTAR"
     gene            8925..>9383
                     /locus_tag="VFD06_03705"
     CDS             8925..>9383
                     /locus_tag="VFD06_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZN02675.1"
                     /translation="MPASDTGRPPADPPAAGRRVAVTAELSIGGGAPLAFLAGPCVIE
                     SEAHAVRMAQALASLSRRMGIPYVFKASFDKANRSSVRSFRGPGLQEGLRILEAVKRE
                     VSVPVLSDIHDPAQAEPAARVLDILQIPAFLCRQTDLLVAAGRTGRTVNIK"
BASE COUNT         1375 a         3429 c         3170 g         1409 t
ORIGIN      
        1 tcgcctcggg gtgcgcgtcg taccaggcgc gcgcttcctt ctctccgacg gcgatcgacc
       61 ccgccacctt ctgatcgacg aagcgcccga tgctgatcgt gcgccggacc tgcgccacga
      121 ggtccgcgtc ggtggcgccg atctccttga gaaacgcggc ggcggtctcg ggggggccga
      181 gcgccgcctt caggcgcgcc gcctcctcct tcgcctcggc gtcggtgacc gtgaccccgg
      241 cctccttcgc cttctggtac aggagctcgt tgtcgatcat gatgtcgagc gctgcctggc
      301 gcaccgcatc gagatcggcg tgcgagcgct cccccgcccc gcgcttgcgg aaaatcatct
      361 gcaccgtgag atcgaagtcc ctccggagca gcggagcgcc gttcaccttc gccaccggcg
      421 cgtcgggacg cggggcggac ggggccgcgg gcccggacgg cttcgcggac ggggcggagg
      481 gcttcaccgg atcgttcgcc ttcttcgcgg gcggggcccc ggccgtcccc tgggtttccg
      541 ccgcccacgc cgccccggcg gcgagacacg ccgccgcgat gattcccacc gccttacttc
      601 ggttctgccg ccagctcatg cgcctcccct ttccacgtct gcacgctgat gatcgccgcc
      661 agccgctcgg cgatctcctt gaacaggatc gccggccccg atttgggatc gtagatcaca
      721 gggggtcgcc cctcatcccc cccctgccgc agacggacgt cgagcgggat cgagccgagg
      781 cacggcaccc cgagcttctc ggccaggcgc gccgctcccc cgtggctgaa gatctccgag
      841 cgctcgccgc agtgcgggca ctcgaagtag gacatgttct ccaccactcc gaggatcggc
      901 acgttcacct tggcgaacat cgccgccgcc ttgcgcacgt ccgagagcgc cacgtcctgc
      961 ggcgtcgcca cgagcacggc gccggtcagc gggatcacct gcgacagcgt gagctgcacg
     1021 tcgccggtgc cggggggcat gtcgatgaac aggtagtcga gggcccccca ctccacgtcg
     1081 ccgagaaatt ggcggatggc gccgtgcagc atcgggccgc gccagacgac cggctcgtcc
     1141 tccttgagga agaaggccat cgacatgagc ttgaggccgt gcacggtcac gggaacgatc
     1201 cgcttctcgc ggatctccgg gcgctccttc accccgagca tctgcaccag gttcgggccg
     1261 tagagatcgg cgtccatcag cccgacggcg gccccggagc gcgcgaacga cagagccagg
     1321 ttcgccgcga cggtggactt gccgaccccc cccttccccg atccgaccgc gaccgtgttg
     1381 gcgacttcgg gaatgagctc cgccggggcg gcgatgctgc gcttcacttt cgcccccatg
     1441 gtgaggtgca cgtccccgat gccggggacc tgccccaggg cggtccggac gtccttttcg
     1501 atctgcccct tcagcggaca gccgggggtg gtgaggttca cggtcagggt gacgtcactc
     1561 ccgtccaccc tcagtccctc gacgagaccc agggtgacga ggtccacgtg aagctccgga
     1621 tccttgacgg tgcggagcgc cgcgagcgcc cgatcgcgga gagcctcgtc gctcatgcgg
     1681 ttcccttccc cgcaggacga tcgcgccacg gggcgctcag tccgcgcagc cccgccgggg
     1741 ccgcgccgcg cgccagcacg agcacctgga acgccccccc catgcgatcg ggaagcacca
     1801 gatccttgag cgcctcgcga ccggcgaggt cctgcgcctc gaatccctcg agcgccccga
     1861 gcgcgagcag gaagtgcgac tgcgacacct ggcccgccac ctcgcacccg gcggtcgccg
     1921 ccgcccgccg caccgcggtg aagtcgacgt gggcggtcag gtcctgctct cccggccgcg
     1981 ccagcacgtc gtcgaccagc cggtgacggt gaaaggcgcg cagcgttccg cggcgccgcg
     2041 ccgggtggaa cagatcctcc gcgcggtcgc catagtccac gaggacgaac cagccgcgtg
     2101 acaggagccg cgcggcgcgg cccacgaagt gcgcggccgc gggggcgacg tccacgatct
     2161 gcccgggcgc gggcgcgagg ccccgggcgt cgaggtcctc gacaaggtcc ggcgtcgaga
     2221 gcggcaggag cttctcccgg aagacgccgt tctcgacgtc gatgtgcacc tcccgcggtc
     2281 cctccggagt cccggtcacg cgatgcaccg ggaaggcgtc gaggacctcg ttggcgaaga
     2341 ggcacccggc aaagccgtcc cgttcgtccg cttcgctttc cagaccctcc agggtgttcc
     2401 aggacaccga cggcgcgccg gccgccccct ctccgcccgg cgccaggagg cgccgctgcc
     2461 tctcccgaag cgccgggctc gtctccacga ggacgtactc gagcgccgcg gcgaacggcc
     2521 ggtcggagcg cgaggcctcc aggacgtcgc gggcgaaacg cccctcgccc gggccgaact
     2581 cgacgaatcg gaaccgcgcg ggcgtgccca gcaaccgcca catctccgcg gcctgacggg
     2641 ccacgagcgt gccgtaggca cggtggacgc cgctgctcgt gaggtagtcg cgtccacctc
     2701 cgccgccgag gccgcgcatg taatagccgt gctcggggtg gtagaggcag atctccatga
     2761 agcgcgcgaa cgggatcggc ccggcggcgc ggatcgcctc gacgaggatc ggttcgagcg
     2821 acgcggcggc cgcgggggcg acggcgttca cttcccggcg gcagcggtgg ccggtgcggc
     2881 cgcgccctga atgggcgcgg caggtgccgc ggcgtccttg accggcgccg ctggtgcggc
     2941 gcctcgtgcg gcgcgagcgc gggcgtcgat gagacgctgg atctgcatga ccccttcgat
     3001 cagggcctcg ggacgcggcg ggcagccggg gatgtagacg tcgaccggga tgagctcgtc
     3061 caccccctgg gtgaccgcgt aggagcggaa cattcccccc gtggaggcgc aggcccccat
     3121 cgcgatcacc cacttcggct ccggcatctg gtcgtagatc cgcttcaccg ccggggccat
     3181 cttcttcacc acggtgccgg cgacgatcat cagatcggcc tggcgcggcg agaagcgcat
     3241 ggcctcggcg ccgaagcggg cgatgtcgta gcgcggcgcc accaccgcca tcatctcgat
     3301 ggcacagcag gccagcccca tcggcatcgg ccagagggag ttcttccgcc cccaggcgat
     3361 gtagtcggca aggcgggtcg tcagcagcca gggttcggcg tcgcgatcga tgtcgctcat
     3421 cgcgtccctc ctgcagcctc cggggcggtc gccgcgacct gcgccttggg gcgcggcttc
     3481 tccagcggtc cccacttgtg cgtggtgtcc ttgcggatgc cacccatctc gagcagccgg
     3541 tccttgtggt agatgagccc ctcgcggctg gtccccaccg tctggtactc cttggtgagg
     3601 aagatcgcct cttcggggca gacctcctcg cagtagccgc acatgatgca gcgcagcatg
     3661 ttgatgtcga acaccttcgg ccccttctcg atgccggtgc cgatctcctg cgcctcgatg
     3721 tggatcgcct tcggggggca gacgtactga cagaggaagc acgccacgca cttgacccgc
     3781 tcctgctcgt cgcggacgag cgccggcagg ccgcggtagc gatccggcat ggcccacttc
     3841 tcctccgggt actgcatcgt caccttcgga cggaacaggt gccggaaggt gatccacatc
     3901 cccttcagga ccgaggggag ataggagcgg gcccagagtc ccttcgggga cgggcgcttc
     3961 accggcttgc cggtcatcgg catggcgggc atgacgcctc ccctccgccc gccgcgtcgc
     4021 gccagcggcg cagcgcctcg cggatcgcct gctccgccag gacggaacag tgccgcttgg
     4081 actccggcag accgcccagc gcgtcggcga cgtcccggtt ggagacccgc tcgacctcct
     4141 ccagccgctt gccggtcacc atcaccgtcg ccatgctccc cgccgcgatg gccgccgtgc
     4201 agccgaacga acgaaagcgc gcctcgatca cctgatcccc ttcgatcctc atcccgaacc
     4261 tgagcacgtc cccatgcgcc ggatccccga cctgggcgac ggccgcgctc ccttcgatgt
     4321 ccccgacgtt gcgcgggtgg cggtagtggt cgagcaacgt ggcgctgtac tcccccgccc
     4381 cctcaccggc cgtcatgcgc ctctcccatg atcttcccgt tcatgctatt gcagtggccg
     4441 tcgcaggggc aacggtgccg ttccggcccg ggctcgaccc tggagcgcgg ccgggtcagc
     4501 cgccgatctg ccacatcttg cgcggcagcc gctcccggta caccgccgtc atgtcgaggc
     4561 tccctcccgg ccccttcgca tccacctcgg cggcgatcgc ccgcaccagt tcgtcgtcgc
     4621 tccccccgcc gcgcaggatc ggccggaggt cgcggtcccc gcgggagaag agacagttgc
     4681 gcaccatccc gtcggcggtg agccggatcc gatcgcagcg gtcacagaag ggctcggtga
     4741 ccgagttgat gaagccgacg tccccggcgc cgtcctcgaa gcggaacacc tccgccggcg
     4801 aggcgtcccg cggccgcggc gccggccgca gcggatgccc cgcctcgatg cgcgcccgca
     4861 cctccgcccc cgggacgatg agcgaagcct cgaaggcctc ccccgccccg agcggcatca
     4921 gctcgatgaa ccgcacctgg cagcggcgcg tgcgcgcgag gtcagcgaag cgcgggatct
     4981 cctggtcgtt gaccccccgg atgaggacga cgttcacctt gatcggcagc gcccccagcc
     5041 gctcgagctc gtcgagcgcg gcgaggaccc ggccgagccc gtcccgacgg ccgaccttcc
     5101 ggtaggtctc cgggtcgagc gagtcgatgt gcacgttgaa gcggcgcacc cccgcgcgca
     5161 ccagcccctc cgcctgctcc ttcaggagaa gcccgttcgt cgtgaccgcg agatcctcga
     5221 cgccgggaag cgcggagagc atgccggcca gccgggcgag atcgcgccgg acgagcggct
     5281 ccccgcccgt cagccggatc tcgcgcacgc cgcgggcggc gagcggccgc accacgcgga
     5341 cgatctcctc gaaggtcagg agctcggacc gctcgtacca agtgatgccg gcgagcggca
     5401 tgcagtacac gcagcgcagg ttgcagcggt cggtcaccga gatgcgcagg ctccgcacgg
     5461 cccggccgaa gcgatcgacg agcggacctg tcggggagac cggttcggga gcgggagcca
     5521 tggcggcggc cgggccgccc ggcgcggcgg gggacacagt tctcatcctc gcggcgggac
     5581 cgggtcgccg tcccggaaga cgtcgaagtc gtcgtcctcc gcggcgggcg ccggcggcgc
     5641 ctcctttttc ttgtagagca gccgatccac cgtctggccc cgcgccacgt ggcgccgcac
     5701 gatctcggcg acgtcggccg gcttcacgcc gctgtagacg accccctcgg gatagatgat
     5761 gacgttcggc ccgtaggtgc acagcccgaa gcacaggcac tcggtgtcct tcaccgtcgt
     5821 gtcgatctgc tggcgcacga actccttgcg gaactcctga cggatgcgtc cggaaccgcg
     5881 ctcgccgccg cacctgggac cgacgcagat caggacgtgg cgggcgtagg gaagattcat
     5941 tcgacctcga ggaccgcctg cagctggagg cacgcgttct gcggcagctc gtcgaccccg
     6001 tgagccagcc gcgtgtgcct gccgcgatcc ccgaagacct tgacgaacac gtcggaggct
     6061 ccttccatga cggtgaacgc gtcgctgaag ccgggggcga tggcgatgtg cccgaccagc
     6121 tgcacgacgc gacggatgcg atcgagatcg tcgatcgcct gcttggcggt cgccagcacg
     6181 ctcagggccg cgagccgcgc cgcgtccgat ccctgctcga ccgtcacctc gcgtccgatg
     6241 cgcccggggt acttcagctt ccctccctcc ttcgcgaatt ttcctgaaat gaacaggagg
     6301 ttcccggaac gcacggtctg gacccagttc ccggtcggct tgccgacttc cggcagcttc
     6361 cagcccaagg actcgatcga tttctcggtt tcgcctggca tcggattctt cctcccgggc
     6421 ggccgcgctt gcgcgcgatc ggccgcgtcc cccaagcatt tgttgcgcaa catcttatca
     6481 gagtcgcgcg accgccgagg cgcggcgttg acccttcgcg cacggctgga ttaccattcg
     6541 ccatgcgacc ccccgacgac gcgagcaccg ccgccgacgc gttgggagtc gtccccgccc
     6601 gctacggttc gacccggttc cccggcaagc cgctcgtcga catcgccggc aagccgatga
     6661 tccggcacgt ctgggagcgg gcgagcgagg cccggctcct caagggggtc gtggtggcca
     6721 ccgacgacga gaggatccgg cgcgtggtcg aggacttcgg cggcgacgtg atgatgacct
     6781 cccccgacca tccctccggg accgaccggc tggccgaggt ggcgcgaagc cgccccgcga
     6841 ccctctacgt caacatccag ggggacgagc cgctggtcga tccggacgat ctcgacgtgc
     6901 tcgtctccgg gatggtcgcc gagccgggct tcgagatggg gacgctttcg gtcccgctcg
     6961 acgatcccag agccgtcgag gatcccaacg tcgtgaaggt cgtgtgcgac gtggccggcc
     7021 gggcgctcta tttctcgcgc gctccgatcc cctatcctcg ccaaccgtcg cagcccacgg
     7081 aggggggccc cgaccctttc gtgttccgcg gctcctggcg gcgccacatc ggcctgtacg
     7141 cctatcggag cgcctttctc agggcgttcg cgacctggga gccgacgccg ctcgagcgga
     7201 cggaggggct ggagcagttg cgcgccctgg agcgtggctc ccccatccgc gtgttcccgg
     7261 cgcgcggccg ctacgtcgcg gtcgacacgc cgggggatct gcgtctcgtc gaaagcgccc
     7321 tcggcgccgt gctgaaagga cggtgagcgt gcccacgaag tacatcttcg tcacgggtgg
     7381 tgtggtctcg tccctcggta aggggctggc cgccgcctcg atcggccgcc tcctggaggc
     7441 gcgggggttc tcggtcgccc tgcagaagtt cgacccgtac ctcaacgtcg atccgggcac
     7501 gatgagtccg ttccagcacg gcgaggtcta cgtcaccgag gacggcgtcg agtcggatct
     7561 cgatctcggc cactacgagc gcttcacctc cacgcagacc tcgcgcgacc acaacttcac
     7621 gagcggcaag atctacgagc acgtcatcgc caaggagcgc cgcggcgact acctcggcgg
     7681 gacggtgcag gtcatcccgc acgtcaccga cgagatcaag gagagcatcc gccgcatcag
     7741 caagggggtg gacatccaga tcgtcgagat cggcgggacc atcggcgaca tcgagtcgct
     7801 gccgttcctc gaggcgatcc ggcagttccg gctggaggtg gggcggagca acgcggtctt
     7861 cgtgcacctg acgctggtgc cgtacatcgg cgccgccggc gagctgaaga ccaagccaac
     7921 gcagcactcg gtgcgcgatc tgcgcgccat cgggatccag ccggacgtgc tgctgtgccg
     7981 gaccgaccgc ttcatcccgc ccgagatcaa gaagaagatc gcgctgttct gcaacgtccc
     8041 ggaggaggcg gtgatcaccg ccaaggacgt cgagaccatc tacgaggtgc cgctggtcct
     8101 cgccaaggag ggtgtggacg cgatcctcct gaaggacctc gaccttccgt accgcgacaa
     8161 gaacatggac gactgggtcg agatggtcgg caaggccaag aacccctcgg acggcgaggt
     8221 ggtggtgggc atcgtcggca agtacgtcgc ctacgaggac tcctacaaga gcctcaacga
     8281 ggcgctcacc cacggcggca tcgccaacga cgcgcgcgtg cgcctgcact ggatcgaggc
     8341 ggaccggctg gtgggcggcg gcatgcagga gcagatggcc ccggtgcacg ggatcctggt
     8401 ccccggcggc ttcggcctgc ggggcgtgga cgggatgatc gaggcgatcc gcttcgctcg
     8461 cgagcgccgc gtgccgttct tcggcatctg cctcggcatg cagtgcgcgg tcatcgagta
     8521 cgcgaggaac gtcgccggcc tcaaggacgc cgactcggcg gagttcaccc ccgagacccc
     8581 gcacccggtc atctacaagc tgcgcgagct cgagggggtg gagcgcatgg gcgggaacat
     8641 gcggctcggc aagtggccct gcctcgtccg gaccgggtcg caggcccaca aggcctatgc
     8701 gcgcacggag atcagcgagc gccaccggca ccgctacgag gtgcaccgca agtacatgga
     8761 cgacctcgag cggcgcgggc tcaaggtgac cggcgagacc cccgacaagc gcttcgtcga
     8821 gatcgtcgag atcccggatc acccgtactt cctggcctgc cagttccacc cggagttcaa
     8881 gtccaagccg atgcagccgc accccctctt ccgatccttc atcgatgcct gcaagcgata
     8941 ccggcagacc gcccgctgat ccgcccgccg cgggacggcg ggtcgccgtc accgcggagc
     9001 tttcgattgg cggcggcgcg cctctcgcgt tcctcgccgg cccctgcgtc atcgagagcg
     9061 aggcgcacgc cgtccgcatg gcgcaggcgc tggcgtcgct gagtcggcgg atggggatcc
     9121 cgtacgtctt caaggcctcc ttcgacaagg cgaaccgttc gtcggtgcgg tcgttccgcg
     9181 ggcccgggct tcaggaaggt ttgcgcatcc tcgaggcggt gaagcgcgaa gtctccgtgc
     9241 cggtcctctc ggacatccac gatccggctc aggcggagcc ggcggcgagg gtgctcgaca
     9301 tcctccagat cccggcgttc ctgtgccggc agaccgacct gctggtggcg gccgggcgga
     9361 ccggacggac ggtgaacatc aag
//
LOCUS       DASRIQ010000152         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_833316, whole genome shotgun sequence.
ACCESSION   DASRIQ010000152 DASRIQ010000000
VERSION     DASRIQ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833316"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VFD06_03710"
     CDS             <1..1065
                     /locus_tag="VFD06_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02676.1"
                     /translation="TPQSPAATPAPGSAGPGGQPAGVPTILYHSNLAGVYLAKHQLDR
                     AEEEIRKGQAIDPQAPPLLLALSVLHEMRGELEKAVDALRALVPADPQAAPGRLVRIA
                     ELYARMGKPEDGIAYFGSLSTSTTPGFEACRQAGLGIVLAAARRGTEAEAAFRKALRE
                     DPSSVLAMQELFALLDGQGRAAELEPLLRDGLKRSPPPAMFHNLLGLVLKRRGDLRGA
                     EMQFRQTLEASPDLVGALANLGGLYLQEGRVPEAVSTLESALDKDPRNVEARTNLIVA
                     LGLEKNLEAARARFDEGEKQGLRAPQFYNAWAYALHVNGRRDEALEMLRASLRLDPRQ
                     ADARRLVQEIEAGVVASPYR"
     gene            1069..1656
                     /gene="ruvA"
                     /locus_tag="VFD06_03715"
     CDS             1069..1656
                     /gene="ruvA"
                     /locus_tag="VFD06_03715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZN02677.1"
                     /translation="MIARLAGTLLEKHPGLLILEVGGVGYELTIPLGTYRAMGEPGSR
                     AELHVHTYVREDTLALYGFATRLEKLLFVRLLGVSGVGPKTAVALLSGLGAELLVEAV
                     RRRDIRRLSGAPGVGRKTAERIALDLADRLDALAESAGTAAPSGVRDDLVSALVNLGY
                     NARAASEAAAGALDGAVPEPPFEDLLRRALRTLSR"
     gene            1776..>2317
                     /gene="ruvB"
                     /locus_tag="VFD06_03720"
     CDS             1776..>2317
                     /gene="ruvB"
                     /locus_tag="VFD06_03720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZN02678.1"
                     /translation="MLDPDPVRDDPAADLALRPRTLDEYIGQERMKANLRIFIQAARS
                     RGEPLDHVLLYGPPGLGKTTIAHILASAMETGLRSTSGPAIERAGDLAAILTNLEGAG
                     VLFIDEIHRLPVALEEILYQAMEDFRLDVVIGQGPGARTVKIDLPRFTLVGATTRMGL
                     LSAPLRDRFGIVHHLDFYPV"
BASE COUNT          326 a          829 c          827 g          335 t
ORIGIN      
        1 actcctcaat ctccggctgc gacccctgct ccgggctcgg cagggccggg aggtcagccg
       61 gcgggtgttc cgaccatcct gtaccactcg aatctcgccg gcgtgtacct ggccaagcac
      121 cagctggatc gcgccgagga ggagatccgc aaagggcagg cgatcgatcc gcaggccccg
      181 cctctgctcc tggccctgtc ggtcctgcac gagatgcgcg gggagctcga gaaagcggtg
      241 gacgccctcc gcgccctggt tccggccgat ccgcaggcgg cccccggccg gctcgtccgc
      301 atcgccgagc tctacgcccg gatgggaaag cccgaggacg ggatcgctta tttcggatcg
      361 ctgtcgacgt cgacgacccc cgggttcgaa gcctgccggc aggcggggct gggaatcgtg
      421 ctggcggccg cgcgccgtgg caccgaggcc gaagccgcct tccgcaaggc gctgcgcgag
      481 gacccgtcct cggttctcgc gatgcaggag ctgttcgcgc tgctcgacgg ccaggggcgt
      541 gccgccgagc tcgagccgct cctgcgggac gggttgaagc gatcaccgcc tcccgcgatg
      601 ttccacaact tgctcgggct ggtgctgaaa cgccggggag acctccgcgg tgcggagatg
      661 cagttccgtc agaccctcga ggcgtctccc gacctggtcg gcgcgctggc gaacctcggc
      721 gggctgtacc tgcaggaggg acgcgttccg gaggcggtga gcacgctcga gagcgcgctc
      781 gacaaggatc cccgtaacgt ggaggcgcgc accaacctga tcgtggcgct cggtttggag
      841 aagaacctgg aggccgcgcg tgcgcgcttc gacgaggggg agaagcaggg gttgagggcg
      901 ccgcagttct acaatgcctg ggcgtatgcg ctccacgtca acggccggcg ggacgaagcg
      961 ctcgaaatgc tccgggcttc gctgcggctc gacccccgcc aggccgatgc ccgccgactg
     1021 gtccaggaga tcgaggcggg ggtcgtcgcg tccccgtatc gctgagcgat gatcgcgcgc
     1081 ctcgcgggca cgctgctcga gaaacaccca ggcctcctga tcctcgaggt cggaggggtc
     1141 ggctacgagc tcaccatccc gctcggcacg taccgggcga tgggggagcc gggcagccgg
     1201 gccgagctgc acgtccacac ttacgtccgc gaggacaccc tggcgctcta cgggttcgcc
     1261 acccgcctcg agaagctcct gttcgtgcgc ctgctcggag tcagcggcgt cggccccaag
     1321 acggcggtgg ccctgctgtc ggggctgggg gccgaactgc tcgtggaggc ggtgcgccgg
     1381 cgggacatcc ggcgtctgag cggcgccccg ggggtcggtc gcaagaccgc ggagcggatc
     1441 gctctcgatc tcgccgatcg cctggacgcg ctcgcggagt ccgccgggac cgcggccccg
     1501 tccggagtgc gcgacgatct cgtctccgcg ctcgtgaacc tcggctacaa tgcgcgcgcc
     1561 gcctccgaag ccgcggccgg ggcgctcgac ggggccgtgc ccgaaccccc cttcgaggat
     1621 ctgctccggc gggccctgag gactctctcg cgctgagcgc tgcgccgcgg ggcatccgga
     1681 tgacgccgac cgacgacaag ccgcgcccac gccccaggcg cccccggccg gaggggaccg
     1741 tggaggaggt gcgccccggc gcgccggcgg accgcctgct cgatccggat ccggtccgcg
     1801 acgatccggc ggccgacctc gcgctgcgcc cgcgcaccct ggacgagtac atcggccagg
     1861 agcggatgaa ggcgaacctc cggatcttca tccaggcggc gcgctctcgc ggcgagccgc
     1921 tcgatcacgt cctgctgtac ggtccgccgg gtctcggaaa gacgaccatc gctcacatcc
     1981 tcgcctcggc gatggagacc ggcctgcgct ccacgtccgg accggccatc gagcgggcgg
     2041 gcgacctcgc cgcgatcctg accaatctcg aaggggccgg cgtcctgttc atcgacgaga
     2101 tccaccggct gccggtggcg ctcgaggaaa tcctctacca ggcgatggag gatttccgtc
     2161 tggacgtggt gatcggacag gggccgggcg cccgcaccgt gaagatcgac ctgccgcgct
     2221 tcaccctggt cggagcgacc acgcgcatgg gcctgctgag cgccccgctg cgcgaccggt
     2281 tcggcatcgt gcaccacctc gatttctatc cggtgga
//
LOCUS       DASRIQ010000153         4665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1366407, whole genome shotgun sequence.
ACCESSION   DASRIQ010000153 DASRIQ010000000
VERSION     DASRIQ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366407"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3249
                     /gene="dnaE"
                     /locus_tag="VFD06_03725"
     CDS             <1..3249
                     /gene="dnaE"
                     /locus_tag="VFD06_03725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZN02679.1"
                     /translation="APKSRHDRTPMGPGKKPYYHLLLLARDQQGYQNLMRLSTAGFLE
                     GYYYRPRIDRELLGQYAGGLIATSTCLGGEIPQAILAGDRKRAEETACEYRELFGAEN
                     FFFEIQDQGIPEEKALNEVLIEMGRRLKIPLLATNDCHFLRKEDHFAHDVLICIQTGR
                     TVKDADRMRFTSEHYLKPAAEMRALFKDLPEAVENTLLVAERCNVTIDKGGLFLPHFQ
                     VPEGTTIEAYFRQVTLAGFEERLESWRRLGPRGVRRAPLEAYRERLEFEIEMIGRMGF
                     PGYFLIVWDFIKHARDHGIPVGPGRGSAAGSLVAYCLRITDIDPLEYDLLFERFLNPE
                     RVTMPDIDIDFCIRGRGRVIDYVRDKYGRDNVAQIITFATMGAKAVIRDAGRGLDIPF
                     GDCDRIAKMVPAALDMTIDRALEESPPLKALYDKDDRIRQLLDVSKRLEGLTRHASVH
                     AAGVVISPRPIVEFSPLGRTKDNEIVTQYAMDEIGAIGLLKMDFLGLKTLTLLSDCVA
                     ALKRDTGDAPDLDHLPLDDPETYALFAAAKTDGVFQFESSGMKDILRKLKPDRFEDLI
                     ALNALFRPGPIGSGMIDDFIERRHGRKPMEYAVPQLEGILGVTYGVIVYQEQVMQIAS
                     QLAGFTLGEADLLRRAMGKKKRDVMAAQRDKFVAGAKGRGIGEKDAKKIFELMEYFAG
                     YGFNRSHSTAYALVAYQTAWLKVHHPRHFMAALLTAEKENTDNIVKYIGACRAMAIPV
                     LPPDLNRSDLDFTVEEDGLRFGLGAVKNVGDLAVTAILEARRRLGRIGSVAALCREVD
                     GRVLNKRVVESLIKAGALDRLGPSRASLAAGVDRAMDAAQKARSDRESGQGGLFGGSA
                     SPSAAEETLGAVEEWARAALLAFEKETLGFYLSGHPLQDESPRFRGLVSHTTASLSEV
                     QRPGPATLAGIVSALKKRRTKKGDAMAVFNLADLDGSVEVIVFPDAFARQRSLLEEDA
                     ALLVTGTVEVDEDRRRLIAETLLPLQQAEEKKAKEVVLSLPDEGLEPGAAERLRTLLS
                     DSPGPCQVFLEVTRPEGFRATLKAAGALKVSPNRDLTLALEGVLGKGSVRFR"
     gene            3308..4480
                     /gene="metK"
                     /locus_tag="VFD06_03730"
     CDS             3308..4480
                     /gene="metK"
                     /locus_tag="VFD06_03730"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZN02680.1"
                     /translation="MGQGRHLFTSESVTEGHPDKIADQISDALLDEVLRNDPTARVAI
                     ETMVTTGLAVVAGEMTTTTYVDVAAVVRDTIRRVGYTRAKYGYDGETCGILTAIDKQS
                     PDIAMGVDTGGAGDQGMMFGFACRETPELMPLPIQTAHRLAQRLADVRRDGTLPYLRP
                     DGKTQVTVEYDGVKPIRVDAVVVSTQHAPEIKTEDLRRDIEAHVIRPVIPEGMRDAKT
                     AIHINPTGRFVVGGPQGDTGLTGRKIIVDTYGGYAPHGGGAFSGKDPTKVDRSAAYMA
                     RYIAKNLVAAELAERVEVQIAYAIGVADPVSIMVDSYGTGRVPDETMTRLVREHFGLK
                     PKEIIESLDLRRPIYLPTAAYGHFGRSGPGFTWEKTDKAEALKRAAGVGAASAVAG"
BASE COUNT          782 a         1604 c         1624 g          655 t
ORIGIN      
        1 gccccgaaga gccgccacga ccgcaccccc atgggtccgg ggaagaagcc ctactaccac
       61 ctgctcctcc tggcgcgcga ccagcagggg tatcagaatc tcatgcgcct ctccaccgcc
      121 ggcttcctcg aggggtacta ctaccggccg cgcatcgacc gcgagctgct ggggcagtac
      181 gccgggggcc tgatcgcgac ctccacctgc ctcggcgggg agatcccgca ggcgatcctc
      241 gccggggatc gcaaacgcgc cgaggagacg gcctgcgagt accgggagct gttcggcgcc
      301 gagaacttct tcttcgagat ccaggaccag gggatccctg aggagaaggc cctcaacgag
      361 gtcctgatcg agatggggcg gcgcctgaag atcccgctcc tggcgacgaa cgactgccac
      421 ttcctccgca aggaggacca cttcgcgcac gacgtgctca tctgcatcca gaccgggcgg
      481 acggtgaagg acgccgaccg gatgcgcttc acgagcgagc actacctcaa gccggccgcc
      541 gagatgcgcg ctctgttcaa ggatcttccc gaggccgtgg agaacacgct gctcgtcgcc
      601 gagcgctgca acgtgacgat cgacaagggg gggctgttcc tgccgcactt ccaggtgccg
      661 gaggggacga ccatcgaagc ctacttccgc caggtgacgc tcgcggggtt cgaggagcgg
      721 ctggagtcgt ggcggcgtct cggccctcgg ggcgtgcggc gcgcccctct cgaggcctat
      781 cgcgagcggc tcgagttcga gatcgagatg atcgggcgga tggggttccc gggctacttc
      841 ctcatcgtct gggacttcat caagcacgcc cgcgaccacg gcatcccggt cgggccgggg
      901 cgcggctcgg ccgccggctc cctcgtcgcg tactgcctgc ggatcaccga catcgacccg
      961 ctcgagtatg accttctctt cgagcgcttc ctgaacccgg agcgggtgac gatgccggat
     1021 atcgacatcg acttctgcat ccgcgggcgc gggcgcgtca tcgactacgt gcgggacaag
     1081 tacggccgcg acaacgtggc gcagatcatc accttcgcga ccatgggggc gaaggcggtg
     1141 atccgcgacg ccgggcgcgg cctcgacatc ccgttcgggg actgcgaccg gatcgccaag
     1201 atggtcccgg ccgcgctcga catgaccatc gaccgggcgc tcgaggagtc gcccccgctc
     1261 aaggcgctct acgacaagga cgatcgcatc cggcagctcc tcgacgtctc gaagcggctc
     1321 gaagggctga cgcggcacgc ctcggtgcac gccgcggggg tggtgatctc gccgcggccg
     1381 atcgtcgagt tctccccgct cggccgcacc aaggacaacg agatcgtcac gcagtacgcc
     1441 atggacgaga tcggcgccat cggcctcctc aagatggact tcctggggct gaagacgctg
     1501 acgctcctct ccgactgcgt cgccgccctg aagcgcgaca ccggtgacgc cccggacctc
     1561 gaccacctgc cgctcgacga ccccgagacc tacgcgctgt tcgcggcggc gaagaccgac
     1621 ggcgtgttcc agttcgagtc gtcggggatg aaggacatcc tgcgcaagct caagccggac
     1681 cgcttcgagg acctgatcgc gctgaacgcc ctcttccggc cggggccgat cggctccggc
     1741 atgatcgacg acttcatcga gcgccgccac gggcgcaagc cgatggagta cgccgtgccg
     1801 cagctcgagg ggatcctcgg cgtcacctat ggcgtcatcg tctaccagga gcaggtgatg
     1861 cagatcgcct cgcagctcgc cggcttcacc ctcggtgagg ccgatctcct gcgccgcgcc
     1921 atgggaaaga agaagcgcga cgtcatggcg gctcagcgcg acaagttcgt cgccggggcg
     1981 aagggtcgcg gcatcggcga gaaggatgcg aagaagatct tcgagctgat ggagtacttc
     2041 gccggctacg ggttcaaccg gtcgcactcg accgcctacg cgctggtcgc ctaccagacc
     2101 gcctggctca aggtgcacca cccgcggcac ttcatggccg ccctgctgac ggcggagaag
     2161 gagaacaccg acaacatcgt caagtacatc ggcgcctgcc gcgccatggc gatcccggtc
     2221 ctgccgcccg atctgaaccg ctcggacctg gacttcacgg tcgaggagga cgggctgcgc
     2281 ttcgggctcg gcgccgtgaa gaacgtcggc gacctggcgg tgacggcgat cctcgaggcg
     2341 cgccggaggc tcggacggat cgggtcggtc gcggcactct gccgcgaggt ggacggccgc
     2401 gtcctcaaca agcgcgtcgt cgagtcgctc atcaaggcgg gcgccctcga tcgcctcggc
     2461 ccgtcgcgcg cctccctcgc cgccggcgtg gaccgggcga tggacgcggc gcagaaggcg
     2521 cgcagcgacc gggagagcgg ccaggggggc ctgttcgggg gatccgcctc cccgtcggcg
     2581 gcggaggaga cgctgggggc ggtggaggaa tgggcgcgcg ccgcgcttct ggccttcgag
     2641 aaggagacgc tcgggttcta cctgtcgggc cacccccttc aggatgaatc gccgcggttc
     2701 cgcggcctcg tgtcgcacac gaccgcgagc ctgtcggagg tccagcgacc gggtcccgcc
     2761 accctcgccg gcatcgtctc cgccctgaag aagcgccgga cgaagaaggg ggacgcgatg
     2821 gcggtcttca atctcgccga tctcgacgga agcgtcgagg tgatcgtctt cccggacgcg
     2881 ttcgcccggc agcgctcgct cctggaggag gacgccgcgc tgctggtgac cggcacggtc
     2941 gaggtggacg aggatcgccg ccggctcatc gccgagaccc tgctgccgct gcagcaggcg
     3001 gaggaaaaga aggccaaaga agtcgtcctg agtctcccgg acgaggggct agagccgggg
     3061 gcggcggagc ggctcagaac gctcctgtcg gacagccccg ggccctgcca ggtcttcctc
     3121 gaggtgaccc gcccggaggg gttccgcgcc acgctgaagg cggccggggc gctcaaggtg
     3181 tcgccgaacc gggacctgac cctcgccctc gaaggggtgc tgggcaaggg gtcggtccgt
     3241 tttcgttgac ttggccgcgc ggactctttt atcctaaccg tctttgccag accgggaggg
     3301 gagggcaatg ggccagggca ggcatctgtt cacgtccgag tcggtgaccg aggggcaccc
     3361 cgacaagatc gccgatcaga tctcggacgc cctcctcgac gaggtcctgc ggaacgaccc
     3421 gacggcgcgc gtcgccatcg aaacgatggt cacgaccggc ctcgccgtcg tcgccgggga
     3481 gatgaccacc accacgtacg tggacgtcgc cgcggtcgtg cgcgacacca tccggcgggt
     3541 cgggtacacc cgcgccaagt acggctacga cggcgagacc tgcggcatcc tgaccgccat
     3601 cgacaagcag tccccggaca tcgccatggg ggtggacacc ggcggcgccg gcgaccaggg
     3661 gatgatgttc ggcttcgcct gccgcgagac gcccgagctg atgccgctgc cgatccagac
     3721 ggcgcaccgc ctggcgcagc gcctggcgga cgtgcgccgc gacgggacgc tgccgtacct
     3781 gcgccccgac ggcaagaccc aggtgacggt ggagtacgac ggggtcaagc cgatccgcgt
     3841 ggacgcggtc gtcgtttcga cgcagcacgc tccggagatc aagaccgagg acctccgccg
     3901 cgacatcgaa gcgcacgtca ttcggccggt gatcccggag gggatgcgcg acgccaagac
     3961 cgctatccac atcaatccga ccggacgctt cgtcgtgggc gggccgcagg gggacaccgg
     4021 gctgaccggc cgcaaaatca tcgtcgacac ctacggcggc tacgcgccgc acggcggcgg
     4081 tgccttctcg gggaaggacc cgacgaaggt cgaccgctcg gccgcctaca tggcccgcta
     4141 catcgccaag aacctggtcg cggcggagct ggcggagcgg gtcgaggtgc agatcgccta
     4201 cgccatcgga gtcgccgacc cggtctcgat catggtggat tcgtacggca ccggccgcgt
     4261 cccggacgag acgatgaccc ggctggtgcg agagcacttc ggcctcaagc cgaaggagat
     4321 catcgagtcg ctcgacctgc gccgcccgat ctacctgccg accgcggcgt acgggcactt
     4381 cggccgcagc gggccgggat tcacctggga aaagaccgac aaggcggagg cgctcaagcg
     4441 cgccgccggc gtcggcgcgg cctccgccgt cgccggctga acgacgccgc atcgcggcgg
     4501 cccgaacgga aatcaacgga acaggagggg gacgtggcgc gcacgaccga cagcgaagtc
     4561 cgggatctca agcaggcgac caaagggaag agccgcgtag agtgggccga tgcgcagatg
     4621 ccggtcctcc gggcgatccg cgagcgcttc gaacgtgaga agccg
//
LOCUS       DASRIQ010000154         5111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1871013, whole genome shotgun sequence.
ACCESSION   DASRIQ010000154 DASRIQ010000000
VERSION     DASRIQ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871013"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFD06_03735"
     CDS             complement(<1..212)
                     /locus_tag="VFD06_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZN02681.1"
                     /translation="MQLGEPGKMQAAINITPLVDVVLVLLIIFMVVAPQMMRKGPEVA
                     LPETVKPHEQGDERGRILVSVDGEGGL"
     gene            complement(213..923)
                     /locus_tag="VFD06_03740"
     CDS             complement(213..923)
                     /locus_tag="VFD06_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZN02682.1"
                     /translation="MSLSELLGNVGLFGGAVMVCLALLSIFSVGMIVDKQRRFRTAVR
                     QSEAFRPVFTKFLHGGDVQDLIAVMPKHRDSHTARVVAAGVLEYDSVRQAGGDPVASL
                     ELVTSALEDSKAETLILMKRGLGFLATIGSTAPFIGLFGTVVGIINAFQSIAATGSGG
                     MAAVSGGISEALVATALGIFVAIPAVVAFNSFTGRLETFHVEINRVSSQLVNCLFTVP
                     EIRVRDGKMAAMKSSRAA"
     gene            complement(920..1309)
                     /locus_tag="VFD06_03745"
     CDS             complement(920..1309)
                     /locus_tag="VFD06_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02683.1"
                     /translation="MSRNVLFVLLLATGVALADEPAGDKPAKGAAEADAKKEAEAKAK
                     EEDAKAKEAADAKAKDEANLGMSILGNQEAPKSLVIVPWKSSEIGKGLGISTMLDDSR
                     QPIDKEVFMRVLSYYDLRSTTTHRRKQ"
     gene            complement(1306..1953)
                     /locus_tag="VFD06_03750"
     CDS             complement(1306..1953)
                     /locus_tag="VFD06_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02684.1"
                     /translation="MSRLPLPIVLASLFLAGCASAPRATAPVEPTEARPEEAKFVRDA
                     GGFTITQSATVGGEERRNYEAAVRLLEEGRDAAAIPLLVKVTEGAPTLTAAHIDLGIA
                     YARTGDLDRAEASLLNALGLNPRHPAALNELGLVQRRKGEFAKARASYQAALAQFPDF
                     HYAHRNLAILCDLFLADRACALEHYEAYHRLAPEDTEVVKWLADLRNRGSQKEKS"
     gene            complement(1950..5072)
                     /locus_tag="VFD06_03755"
     CDS             complement(1950..5072)
                     /locus_tag="VFD06_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02685.1"
                     /translation="MRRLAPIVLAPALVVGCATHKPAGTLAELRHVDPDLQDATVDEG
                     LDQAVQAYRRYLEETPVTAMTPEAIRRLADLQVEKQFGIRAGGGMPQPIASPLAAETP
                     VGAPASAVTPAPGDGPRETQQEFEQRASAESTDLPAGDASASFPGTAPGEAPPQGPLE
                     AIALYERLLKEYPSYQNSDQVLYQMARAYDELGRTEQAMEIMDRLIRANPQSLHHDEV
                     QFRRAEYYFMRRKFRDAENAYSSITAKGAASTFYELALYKLGWTFYKQDLYDEALQQY
                     MGLIDYKVSVGYDFDQQHEEEDERRVADTFRVISLAFSNLGGPDAVQKYFTTAGERPY
                     ENRVYANLGEHYLEKLRYDDAAKTYKAFVALHPFHRAAPQFSMRVVETFTKGGFPKLV
                     LESKREFASLYGLQSDYWKHNKTDEAPEVVEYLKGNLKDLATHYHAQYQGTKKADEKL
                     AHYGEARRWYGDYLASFPTDADSPGINYRLADLQLENKDFGDAAKQYERTAYDYPAHS
                     QSAAAGYAAVFSHREQLKTAGEAERDAIKRETVASSLKFADAFPDHQHAAAILGAAAD
                     DLYEMKDYAIAVATARRVIETWPAADAAIRRSAWIVVAHGSFELAEYPQAEQAYTQVL
                     AATPGDDESHAALVDTLAASIYKQGELAREAQDYRAAADHFLRIRTAAPTSTIRPASE
                     YDAGAALMALKDWTAAAAALEAFRADYPEHKLHGEATRQIALAYKESGQLSRAAGEYE
                     RLASQSEDPTVRGESLLVAGDLYAQSEARDRALEVYGRYVRDFPRPIETALETRFKIA
                     EMHKAAHDDGLYRKQLREIVKLDADAGSARTGRTRTLAGRSALVLSEQTYGEFTAVKL
                     TQPFEKSLQEKKQRMDAVIDVMDGLVAYEIADVTAAAAYYIAETYSDFSRSLVESERP
                     DDLTPAQLAEYNLALEDEAFPFEEKAIGFHEKNLEMMRAGSFNAWVEKSLGRLAELVP
                     GRYAKKEVSSGLVKALDGYVYRTPLSQIPVPAPASSDAAPPAEPATAPDSAPAPPDSS
                     PAPAPTEGGR"
BASE COUNT          697 a         1731 c         1779 g          904 t
ORIGIN      
        1 agtccgccct ccccgtcgac cgagacgagg atccggcctc gctcgtcccc ctgctcgtgc
       61 ggcttcaccg tctccggcag ggcgacctcc ggtcccttcc gcatcatctg cggcgcgacg
      121 accatgaaga tgatcagcag caccagcacc acgtcgacga gcggcgtgat gttgatggcg
      181 gcctgcatct ttcccggttc gccgagttgc atttaggccg ccctcgagga cttcatcgcc
      241 gccatctttc cgtcccggac cctgatctcc ggaaccgtga acaggcagtt cacgagctgc
      301 gacgacaccc ggttgatctc gacgtggaag gtctcgagcc ggccggtgaa gctgttgaag
      361 gcgacgacgg ccgggatggc gacgaagatc ccgagcgcgg tggcgaccag cgcctcggag
      421 atgccgcccg agaccgccgc catcccgccc gacccggtcg ccgcgatgct ctggaaggcg
      481 ttgatgatcc cgacgacggt gccgaagagg ccgatgaacg gtgccgtcga gccgatggtg
      541 gccaggaatc cgaggccccg cttcatcagg atcagcgtct cggccttgga gtcctcgagc
      601 gcgctcgtga cgagttcgag cgaggccacc ggatcgccgc cggcctggcg cacgctgtcg
      661 tactcgagga cgccggccgc gacgacccgc gccgtgtggg agtccctgtg cttcggcatt
      721 acggcgatca gatcctgcac gtcaccgccg tgcagaaact tcgtgaacac cggcctgaac
      781 gcctccgact ggcgcacggc cgttcggaag cggcgctgct tgtccacgat catccccacc
      841 gagaagatcg acagcagggc gagacagacc atcaccgcgc ctccgaagag gccgacgttt
      901 cccagcagct ccgagagact cattgcttcc tcctgtgcgt cgtggttgac ctcaggtcgt
      961 agtaactgag aacccgcatg aacacttcct tgtcgatcgg ctgccgggag tcgtcgagca
     1021 tcgtcgagat gccgagcccc ttgccgatct ccgagctctt ccaggggacg atgaccagcg
     1081 acttgggcgc ttcctggtta ccgagaatcg acatgcccag gttcgcctcg tctttggcct
     1141 tggcgtccgc cgcctccttg gccttggcgt cctcttcctt cgccttggcc tccgcctctt
     1201 tcttggcgtc cgcctcggcg gcacccttgg cgggcttgtc cccggccggc tcatcggcca
     1261 gagcgacgcc cgtcgccagc aggagcacga acagcacgtt gcggctcatg acttctcctt
     1321 ttggcttccg cggttgcgaa ggtcggccag ccacttgacg acctccgtgt cctcgggagc
     1381 gagtcggtga taggcctcgt agtgctccag cgcgcaggca cggtctgcga ggaacaggtc
     1441 gcagaggatg gccaggttcc ggtgcgcgta atggaagtcg gggaactggg ccagggccgc
     1501 ctggtagctg gcgcgcgcct tcgcgaactc ccctttgcgc cgctgcacca ggccgagctc
     1561 gttgagcgcg gcgggatgcc ggggattcag ccccagggcg ttgagcaggc tggcctcggc
     1621 gcggtcgagg tcgccggtgc gcgcatacgc gatgccgaga tcgatgtgcg cggccgtgag
     1681 cgtgggcgcc ccttcggtca ccttgaccag cagggggatg gccgcggcgt cgcggccctc
     1741 ctcgagcagc cgcaccgccg cctcgtaatt ccgccgctcc tcgcccccga ccgtcgcgct
     1801 ctgcgtgatg gtgaatcctc cggcatcgcg gacgaacttc gcctcctcgg ggcgcgcctc
     1861 ggtcggttcg accggcgcgg tcgcgcgcgg cgcggaggcg cagccggcga ggaagagcga
     1921 cgcgagcacg atcggaaggg ggaggcggct catcggcctc cctccgtcgg cgccggggcc
     1981 ggagaggagt ccggcggggc cggcgccgag tccggcgccg tggccggctc ggcgggcggc
     2041 gcggcgtcgg agctggccgg cgccgggacc gggatctgcg agaggggcgt gcggtacacg
     2101 tacccatcga gagccttgac gaggccgctg ctgacttctt tcttcgcgta gcgccccggc
     2161 accagctcgg cgagcctgcc gaggctcttc tcgacccagg cgttgaagct gccggcccgc
     2221 atcatctcca ggttcttctc gtggaagccg atggccttct cttcgaacgg gaacgcctcg
     2281 tcctcgaggg ccaggttgta ctcggccagc tgcgcgggcg tcaggtcgtc cggccgctcc
     2341 gattccacga gcgaacggct gaaatcggag taggtctcgg cgatgtaata ggcggcggcg
     2401 gcggtcacgt cggcgatttc gtacgccacc agtccgtcca tgacgtcgat cacggcatcc
     2461 atgcgctgct tcttttcctg caggctcttc tcgaacggct gcgtgagctt cacggccgtg
     2521 aactctccgt aggtctgctc cgagaggacc agggcggagc gcccggcgag cgtccgggtc
     2581 cggccggtcc gcgccgatcc ggcgtcggcg tcgagtttga cgatctcccg gagctgcttc
     2641 cggtagagcc cgtcgtcgtg cgccgccttg tgcatctcgg cgatcttgaa gcgggtctcg
     2701 agggccgttt cgatcggccg ggggaagtcc ctcacgtagc gcccgtacac ctccagcgcg
     2761 cgatcgcggg cctcggactg cgcgtacagg tcgccggcga cgagcagcga ctcgccgcgg
     2821 accgtgggat cctcggactg cgacgcgagg cgctcgtatt cccccgcggc gcgcgacagc
     2881 tggccgctct ccttgtaggc gagggcgatc tgccgggtcg cctcgccgtg cagcttgtgc
     2941 tcggggtagt cggcgcggaa cgcttcgagc gccgccgccg cggccgtcca gtccttcagc
     3001 gccatcaggg ccgcccccgc gtcgtattcg gacgcgggac ggatcgtgga cgtcggcgcg
     3061 gccgtgcgaa tcctcaggaa gtggtcggcc gcggcccggt agtcctgggc ctccctggcc
     3121 agctcgccct gcttgtagat ggaggcggcg agagtgtcga cgagcgcggc gtgcgattcg
     3181 tcatccccgg gggtggccgc cagcacctgc gtgtaggcct gttcggcctg cgggtactcg
     3241 gcgagctcga acgagccgtg cgcgacgacg atccaggcgg agcgccggat ggccgcgtcc
     3301 gcggccggcc aggtctcgat gacgcgccgg gcggtcgcca ccgcgatggc gtagtccttc
     3361 atctcgtaga gatcgtccgc ggccgctccc aggatcgccg cggcgtgctg gtggtcgggg
     3421 aaggcgtcgg cgaatttcag cgagctggcg accgtctcgc gcttgatggc gtcacgctcc
     3481 gcctcgcccg ccgtcttcag ctgctcgcga tgggagaaga cggcggcgta gcccgctgcg
     3541 gccgactgcg aatgcgccgg gtagtcgtag gcggtgcgct catactgctt cgccgcgtcg
     3601 ccgaagtcct tgttctccag ctgcaggtcc gccaggcggt agttgatccc gggggaatcg
     3661 gcgtcggtcg ggaacgacgc gaggtaatcg ccgtaccagc gccgggcctc gccgtagtgg
     3721 gcgagcttct cgtccgcttt cttcgtcccc tggtactggg cgtggtagtg cgtggcgagg
     3781 tccttcaggt tgcccttcag atactccacc acctccggcg cctcgtccgt cttgttgtgc
     3841 ttccagtagt cggactggag tccgtagagg gacgcgaact cccgcttcga ctccagcacg
     3901 agtttgggga acccgccctt ggtgaacgtc tccacgacgc gcatgctgaa ttgcggagcg
     3961 gcgcggtgga acgggtgcag cgcgacgaac gccttgtacg tcttcgccgc gtcgtcgtag
     4021 cgcagctttt ccaggtagtg ctcgccgagg ttcgcgtaca cccggttttc ataggggcgc
     4081 tccccggcgg tcgtgaagta cttctggacg gcatccggcc cgccgaggtt gctgaaggca
     4141 aggctgatga cccggaaggt gtcggcgacg cggcgttcgt cctcctcctc gtgctgctga
     4201 tcgaagtcgt agccgaccga caccttgtag tcgatgagcc ccatgtactg ctgcagcgcc
     4261 tcgtcgtaga gatcctgctt gtagaaggtc cagccgagct tgtagagggc gagctcgtag
     4321 aaggtcgagg cggccccctt ggcggtgatc gaggagtagg cgttctccgc gtcgcggaat
     4381 ttccggcgca tgaagtagta ctcggctcgc cggaactgca cctcgtcgtg gtgcagggac
     4441 tgcggattcg cccggatcag gcgatccatg atctccatgg cctgttccgt gcggcccagc
     4501 tcgtcgtagg cccgcgccat ctgatagagg acctgatcgc tgttctggta gctcgggtac
     4561 tccttgagca gccgctcgta cagggcgatc gcctcgagcg gaccctgggg aggagcctcg
     4621 ccaggtgcgg tgcccgggaa ggacgcagac gcgtcgcccg cgggcaggtc ggtgctctcc
     4681 gcggacgcgc gctgctcgaa ctcctgctgg gtttcccgcg ggccgtctcc cggggcgggt
     4741 gtcaccgcgg aagccggcgc gcccacgggc gtctcggcgg ccagcggaga ggcgatcggc
     4801 tggggcatcc cgccgccggc gcggatgccg aactgcttct cgacctggag atccgccagg
     4861 cggcgaatcg cctcgggagt catcgccgtg accggcgtct cctcgaggta ccgccggtag
     4921 gcctgcacgg cctgatcgag gccctcgtcg accgtggcgt cttggaggtc cgggtccacg
     4981 tgacgcagct ccgccagcgt tccggccggc ttgtgcgtgg cgcagccgac gacgagcgcc
     5041 ggagcgagca cgatcggagc gaggcggcgc atcatttctc ctgcgctccg ggctgggcgc
     5101 gcgcggcccg g
//
LOCUS       DASRIQ010000155         4307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2676844, whole genome shotgun sequence.
ACCESSION   DASRIQ010000155 DASRIQ010000000
VERSION     DASRIQ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2676844"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFD06_03760"
     CDS             <1..550
                     /locus_tag="VFD06_03760"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZN02686.1"
                     /translation="RLMAPVLSFTAEEVWQALHRHPDGEALERTVHVEEFPKALTLPA
                     DADLIGRWDRLFEVREDALKALEIARGAGTIGTGLEAEVILEAPADLRDLLQRHASEL
                     ATIFIVSRVVLGKAPDGAHESARFPGLRIGVRRAQGTKCERCWMITTDVGGDPELPGL
                     CARCVRTVRALLDSRAAAEPGR"
     gene            547..1104
                     /gene="lspA"
                     /locus_tag="VFD06_03765"
     CDS             547..1104
                     /gene="lspA"
                     /locus_tag="VFD06_03765"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZN02687.1"
                     /translation="MNPRVRWLALSAGVLALDQATKALVQARLPLHDSRTVIPDLFSL
                     THVTNRGALFGLLHDLPDPWRAVLFTVVPFAAILLILYFQGRTASGDRLAHGGLALIL
                     GGAAGNFLDRLRLGYVVDFLDVYVGTHHWPAFNVADSAICIGVGLLVLDLLRPHRRPR
                     PVDAGASAPAESSGSPGSANAPGPL"
     gene            1085..1906
                     /gene="lgt"
                     /locus_tag="VFD06_03770"
     CDS             1085..1906
                     /gene="lgt"
                     /locus_tag="VFD06_03770"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZN02688.1"
                     /translation="MHPVLFDLPLGRFGSFTVGTYGLFYALGFLLALRLAVHYARRDG
                     IEPGRILDLGIVGLLAGFVGAKLLLYLIDARYYFAEPARMLENLRSAGVYYGGLGLAV
                     LAGVLYVRRHRLPLGKVADLCAPALSLGQAIGRLGCFLAGCCYGRACDLPWAVTFSDP
                     RAAELTGVPIGIGLHPTQLYHAAADLLTLAVTMRLMRRRRFDGQVFWAWLLMYALLRA
                     IVEIWRGDTARGVFLDGRMSTSQIISIPVVLVSAAMLWRLARRTAPEAPRQRATR"
     gene            1903..2916
                     /locus_tag="VFD06_03775"
     CDS             1903..2916
                     /locus_tag="VFD06_03775"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZN02689.1"
                     /translation="MTAAARGAAGGILIELSESRRGERLDQALPDLVPGQSRTSLQRL
                     IREGHVEVDGRKARASYRIRGGERVQVTLPPPIPSGLEAEAIPLDILFEDEDLVVVNK
                     PAGMTVHPGAGVKRGTLVNALLHHGAGLSAIGGVERPGIVHRLDRDTSGVMVVARHDR
                     AHRDLAAQFKARSVEKVYEALVWGRPGAFAGRVEQPIGRHKTARVRMAVRRDGRPSVT
                     RWTIARAFGPATLLEVRPQTGRTHQIRVHLAHLGHPVVGDPLYGGRRRPAPSRSASAA
                     TRAALQAIEGFAGLALHAARLAFDHPKDGRRMRIDAPRPQPLADLLARLEEAGAGTPE
                     DSR"
     gene            2913..3794
                     /locus_tag="VFD06_03780"
     CDS             2913..3794
                     /locus_tag="VFD06_03780"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HZN02690.1"
                     /translation="MSDRTFSIIGAGRLGRTLGRLLVDQGYKPAGITSRTLRSARQAA
                     AFIGGGEPGTSNARGAARGDLVLITTPDGAIAPLARDLAASRLSWSRRVVAHTSGALS
                     SAALEPLARQGARTASIHPLASVADPRTAIATLKGSPFAIEGDAAAVSVLRRLVQGLG
                     GVPVTIPRQAKVLYHLVACLLSNDLVALLSIGLDAARGLGLSRRDAARLYVPLVRGTV
                     ENVARLGPVKALTGPVSRGDVTTLRLHGEALRTLPPDLRRLHRILALRSTALALEART
                     INPETAVQLARLLSALP"
     gene            complement(3885..>4307)
                     /locus_tag="VFD06_03785"
     CDS             complement(3885..>4307)
                     /locus_tag="VFD06_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02691.1"
                     /translation="TYTLTSNDAAANDSTFTRGFALISNTFNLTAPTGVTLGSKSSLA
                     LNCVAHDKQQFPAFTDVTVGQPVSFKIEAVDRAGNLATWPNQPQGTFNQTNVACNGDE
                     CACCFLLSSLPGAECVDRPGLLGTPGSGFENGLCVDLL"
BASE COUNT          537 a         1556 c         1549 g          665 t
ORIGIN      
        1 ccggctcatg gcgccggtgc tgtcgttcac cgcggaggag gtctggcagg cgcttcaccg
       61 gcaccccgac ggcgaggcgc tcgagcgcac cgtgcatgtc gaggagttcc cgaaggcgct
      121 cacgctccct gccgacgccg atctcatcgg acggtgggat cggctgttcg aggtgcgcga
      181 ggacgcgctc aaggcgctcg agatcgcccg cggcgcgggg acgatcggca ccggcctcga
      241 ggccgaggtg atcctcgagg cgccggccga cctgcgcgat cttctgcagc ggcacgcctc
      301 cgagctcgcg accatcttca tcgtctcccg ggtcgtcctc ggaaaggcgc cggacggggc
      361 gcacgagagc gcccgttttc ccggcctgcg aatcggagtg cgccgggccc aagggacgaa
      421 gtgcgagcgc tgctggatga tcacgaccga cgtcgggggc gatccggagc ttccggggct
      481 gtgcgcccgc tgcgtccgga cggtgcgggc cctcctcgac tctcgggccg ccgccgagcc
      541 gggccgatga acccgcgcgt ccggtggctc gccctgagcg ccggggtgct ggcgctcgac
      601 caggcgacca aggcgctggt ccaggcgcgg ctgccgctgc acgactcgag gacggtcatc
      661 cccgacctgt tcagcctgac gcacgtcacc aaccgggggg cgctgttcgg cctgctccac
      721 gacctgcccg atccgtggcg cgcggtgctg ttcaccgtcg tcccgttcgc ggcgatcctc
      781 ctcatcctct atttccaggg acgcaccgcg tccggggatc gcctggcgca cgggggcctc
      841 gccctgatcc tcggcggcgc ggcggggaac ttcctcgacc gcctgcggct cggctacgtc
      901 gtcgatttcc tcgacgtcta cgtcggcacg caccactggc cggccttcaa cgtcgccgat
      961 tcggcgatct gcatcggcgt cggcctgctc gtcctcgacc tcctgcgtcc gcaccggcgc
     1021 ccgcgcccgg tggacgcggg cgcctccgct cccgcggagt cctccggcag ccccggatcg
     1081 gcgaatgcac ccggtcctct ttgatcttcc gctcggccgc ttcgggtcct tcaccgtcgg
     1141 gacctacggg ctcttctacg ccctgggatt cctcctggcg ctgcgcctgg cggtccacta
     1201 cgcccgccgg gacgggatcg agccgggacg catcctggat ctcgggatcg tgggattgct
     1261 cgccggcttc gtcggggcga agctgctcct gtacctcatc gacgcgcgct actacttcgc
     1321 agagccggcc cgcatgctcg agaacctccg gtcggccggc gtgtactacg gggggctggg
     1381 actggcggtc cttgcgggcg tgctctacgt gcgccggcac cggctgccgc tcggcaaggt
     1441 ggccgatctc tgcgccccgg ccctgtcgct cggacaggcg atcggccggc tcgggtgctt
     1501 cctcgccggc tgctgctacg gccgcgcctg cgatctcccc tgggcggtga cgttcagcga
     1561 tccgcgcgcc gcggagctga ccggcgttcc gatcgggatc gggctgcacc ccacgcagct
     1621 ctaccacgcg gccgccgacc ttctgaccct ggcggtgacc atgcgcctga tgcggcggcg
     1681 ccgcttcgac ggccaggtct tctgggcctg gctcctgatg tacgccctgc tgcgggcgat
     1741 cgtcgagatc tggcgcgggg acacggcgcg cggcgtgttc ctcgacggga ggatgtccac
     1801 ctcccagatc atctcgatcc ctgtggtcct cgtctccgcg gcgatgctgt ggcgcctcgc
     1861 ccggcgcacc gccccggagg cccccaggca gcgcgcgacc cgatgaccgc ggccgcgcgg
     1921 ggcgcggcgg gagggattct gatcgaactg tcggagtcac gccgcggcga acggctcgat
     1981 caggcgctcc ccgatctcgt tcccgggcag agccgcacgt cgctgcagcg gctcatccgc
     2041 gaaggccatg tcgaggtgga cggccggaag gcgcgcgcct cgtaccggat ccgcggagga
     2101 gagcgcgtgc aggtgacgct cccgccgccg atcccgtcgg ggctcgaggc ggaggcgatc
     2161 ccgctcgaca tcctcttcga ggacgaggat ctcgtggtcg tcaacaagcc ggccgggatg
     2221 acggtgcacc cgggggcagg ggtgaagcgc ggcacgctcg tgaacgcgct tctgcaccat
     2281 ggcgcggggc tctccgccat cggcggcgtg gagcggcccg gcatcgtcca ccggctcgac
     2341 cgggacacgt ccggggtcat ggtcgtggcg cgccacgatc gcgcccaccg ggatctcgcc
     2401 gcgcagttca aggcacggag cgtcgagaag gtctacgagg cgctggtctg gggacggccg
     2461 ggcgccttcg ccggccgcgt ggaacagccg atcggtcgcc acaagacggc ccgcgtccgg
     2521 atggcggtgc gccgcgacgg ccgcccgtcg gtcacccgct ggaccatcgc gcgggcgttc
     2581 ggcccggcga ccctgctcga agtgcgcccg cagaccggac ggacgcacca gatccgggtg
     2641 cacctggcgc acctcgggca cccggtcgtc ggcgaccccc tctacggcgg ccgccgccgg
     2701 ccggcgccgt cgcgatcggc atccgcggcg acgcgcgcgg cgctccaggc catcgaaggg
     2761 tttgcggggc tcgccctcca tgccgcacgt ctggcgttcg accatcccaa ggacggccgg
     2821 cggatgcgca tcgacgcgcc gcgaccgcag ccactggccg accttcttgc acgcctcgag
     2881 gaagcggggg cggggacccc ggaggactcg cgatgagcga tcggaccttc agcatcatcg
     2941 gcgcaggacg gctcgggcgg acgctcggac ggctcctcgt cgatcaggga tacaagccgg
     3001 ccggaatcac ctcgcgaacg cttcgctccg cgcggcaggc ggcggcgttc atcggcggcg
     3061 gcgagccggg gaccagcaac gcacgcggcg ccgcgcgcgg ggacctggtc ctgatcacga
     3121 cgccggacgg ggcgatcgct cccctggcga gggatctcgc cgcctcccgg ctgtcctggt
     3181 cgcggcgggt ggtggcgcac acgagcggcg ccctttcctc cgccgccctc gagccgctgg
     3241 cgcgccaggg ggcgcgcacc gcctccatcc atcctctggc gagcgtggcc gatccgcgca
     3301 cggcgatcgc aacgctgaaa ggaagcccgt tcgcgatcga gggggacgct gccgccgtgt
     3361 cggtgttgcg gcgcctggtg caggggctcg gaggcgtccc ggtcacgatc ccacggcagg
     3421 cgaaggtgct ttatcacctg gtggcctgcc tgctgtcgaa cgacctcgtg gcgctcctgt
     3481 cgattggcct cgacgccgcc cgggggctcg gcctcagccg tcgggacgcg gcccgcctct
     3541 acgtgccgct cgtgcgcggg acggtcgaga acgtggcgcg gctgggcccc gtcaaggcgt
     3601 tgacggggcc ggtgtcacgc ggcgacgtga cgacgctgcg gctgcacggc gaagcgcttc
     3661 ggacccttcc tccggatttg cgccggctgc accggatcct ggcgctccgc tcgaccgccc
     3721 tggcgctcga agcccggacg atcaaccccg agaccgccgt tcagctggcc cggcttctca
     3781 gcgctcttcc ttgatcacgg gggtttcggg ctttagattt cgcgccggcg ggcgtgtgcg
     3841 cccgcggaga tccactgcgg atgaggcctc ggacgaccag aagtctagag gagatcgacg
     3901 cagagaccgt tctcgaaacc ggacccggga gttccgagca gcccgggtcg atccacgcat
     3961 tccgctccgg gcaggctgct gagcaggaag cagcaggcgc actcgtcccc gttgcaggcg
     4021 acgttcgtct ggttgaaggt cccctgcggc tgattcggcc aggtggcgag gttgccggcg
     4081 cgatcgaccg cctcgatctt gaagctgacc ggctgcccga ccgtgacgtc cgtgaaggcc
     4141 gggaactgct gcttgtcgtg ggcgacgcag ttgagggcca gcgacgactt cgacccgagc
     4201 gtgacgccgg tgggcgccgt caggttgaac gtgttgctga tgagggcgaa gccgcgggtg
     4261 aacgtagaat cgttcgccgc cgcgtcgttg ctcgtcaggg tgtaggt
//
LOCUS       DASRIQ010000156         5414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1626384, whole genome shotgun sequence.
ACCESSION   DASRIQ010000156 DASRIQ010000000
VERSION     DASRIQ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626384"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..3638)
                     /locus_tag="VFD06_03790"
     CDS             complement(78..3638)
                     /locus_tag="VFD06_03790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HZN02692.1"
                     /translation="MRRPSRPGCARHAAAALLVSLALAAGAASPAAPKLPTSAPRDSR
                     GPQYVEGEILVKFRREALAASRGVVRGEVAGALQRRFASGAELWRLGAGVTVPEALVR
                     LENAPDVEYAEPNYLLYATRTPDDPLFQQQYALLNTGQAGGSAGSDIDATGAWDVSIG
                     GALVVVAIMDSGIDLDHPDLAPNLWTNPDEIAGNLVDDDGNGLVDDVHGWDFVNHDDE
                     PRDDNGHGTHVAGIVAAAGDNGLGIAGVAWRASLMPVKFLDATGAGDTAGAIQAIDYA
                     ARMGARIINASWGGGGFSMAMLESIRDAGLHEVLFVAAAGNDGHDSDIVPAFPAGFDT
                     PNVIAVAATDRHDRLAPFSNHGATTVDIAAPGVQIMSALPGGFYGQSSGTSMATPHVS
                     GVAALILGFAPGMGAGPLRRRILEHAEPVAALAGLVAVGGRVNALRCLLDADTTPPGP
                     IADLRVVEPLSDGVVLAWTDTGDDSGGGAAATYDLKVSEEPFDVAGFEGAPRFPVPGP
                     PLPAGSSQTQEVFGLQPARTYYLGLRAVDEWGNAGPPGFATASTLPPPALEATPDSSS
                     AALRSGHTATSLLTLRNSSEGTLDWSLGRPNLRPTPARAATGLERWGGADGFGYVFID
                     SDEPGGPVFAWRDIGATGHSALIGADDRMSESIPLGFAFPFYGQTFTSVRIADNGFLS
                     FTHADSPFENQPLPNPGAPKNLVAGYWDDLFVWGLQDVVWLAEPGAFTVQYNAVLRYG
                     GGGPYTFQMVLFDSGEILFQYLEMVEQMDSATIGIQDASGTAGLPIGFNAPYVHDRLA
                     VRLLIQKDWVSAAPRSGRLRSGESASIPLTFDASGLAAGDYEGRVPILSNDPDRPRIE
                     HPVFLSVEDARAIAAEPAAIDFGNVFTKDGARHVVEIVSSGSRPLTVTAAVPDDPAVL
                     ADFAPFLLAPGERRLLVLDWFPAAPGDLQAGLRIESDAENAPSLVVPLAGIALNPPRA
                     AAIWPPAQVECAGPEGSEVVLDGSRSESADSRPGRTIEIVLYEWIENLGAADEALLGT
                     GRRITATLPPGTHRLGLRVTDSLGETDSIESTITVADTIPPELRVAASPAILKPPNNR
                     MVPVHVTWSAVDACSGVVPVRLIEVLSSEPDDMSDGGDIAGIEPGTADDTLLLRAERD
                     AGAPRRLYTLRYVATDAAGLSTQGEAVVTVTGGHKRTARR"
     gene            complement(3748..>5414)
                     /locus_tag="VFD06_03795"
     CDS             complement(3748..>5414)
                     /locus_tag="VFD06_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN02693.1"
                     /translation="YRPDLDGDGACSDNCPSTPNPAQQDGDGDGTGDACDVCPVHADP
                     VQEDQDGDRAGDACDTCPVTPDPAQTDADGDRLGDACDNCAAVANADQTDSDADGSGD
                     ACQPTIGIDAVVEDGGEFLEVRFRARDPQNETLAGKILLLRQDATFEIENLATTLSCA
                     LGWLPEGRTGEGIGYIAGFGYAALFDLDTGLGCSDGAADYLLGPGPCSEPTAPFDVYF
                     DMPPAPATVCIRRADGSSDDFEVTIDAYGQSVARLLTTAALVRATPDASPLARRMEIA
                     DLEPGSNYVVVVQATDGNTRPVRASAPFLYGGERWLVLNVPPQAGALWPQAPVECAGP
                     GGSAVVLDASPSSDDDSPAGTPSDIVLYEWFENAGGASESLLGTGPRITATLPLGSHR
                     VALRVTDSLGSTDTIESTIVVDDTTPPALSVTAAPLVLWPPDHRMVPVNVFWSAVDAC
                     SRDVEVLVTAGASSEPDDARGGKDGSTTGDIAGLEPGTADGSVLLRVERNVAGPGRVY
                     TLTYVATDSSGRAATGTATVTVPTDRPDRASRSKRDDRPRAGVRRR"
BASE COUNT          770 a         1856 c         2030 g          758 t
ORIGIN      
        1 acatctggcg gctcggccgc ggtgattcga accgggcgat cgtcggcgga gtcgcctgat
       61 ccggggcggg tcgaggatca gcggcgcgcg gtccgcttgt ggccgcccgt cacggtgacg
      121 accgcctcgc cctgcgtgga gaggccggcg gcgtcggtcg cgacgtaacg gagggtgtaa
      181 agacgcctcg gcgcgcccgc gtcccgctcc gcgcgcagca gcagcgtatc gtcggcggtc
      241 ccgggttcga tgccggcgat gtcgccgccg tcgctcatat cgtccggctc gctgctcagg
      301 acttcgatca ggcggaccgg gaccacgccg gagcaggcgt ccacggcgct ccaggtcacg
      361 tgcaccggga ccatgcggtt gttcggcggt ttgaggatcg cgggagaggc tgcgaccctc
      421 agctccggcg ggatggtgtc ggcgacggtg atcgtgctct cgatgctgtc ggtctcgcca
      481 aggctgtcgg tgacccgcag gcccaggcgg tgggtcccgg gcggcagcgt cgccgtgatc
      541 cgccggccgg tcccgagcag cgcttcgtcc gcggcgccga ggttctcgat ccactcgtag
      601 aggacgattt cgatcgttcg gccgggacgg gagtcggcgc tctcggagcg cgacccgtcg
      661 agcacgacct ccgagccttc cggaccggcg cactcgacct gcgccggagg ccagatcgcg
      721 gcggcccggg gcgggttcaa cgcgattccc gccagcggca cgacgagcga gggagcgttc
      781 tcggcatcgc tctcgatgcg caggccggct tggaggtcgc cgggcgccgc gggaaaccaa
      841 tcgagcacca gcagacgccg ctcgcccggg gcgaggagga acggcgcgaa gtcggcgagg
      901 acggccggat cgtccggaac ggcggcggtg accgtcaacg gccggctgcc gctgctgacg
      961 atctcgacga cgtgtcgcgc tccgtccttc gtgaacacgt tgccgaaatc gatcgcggcc
     1021 ggctcggcgg cgatcgcccg cgcgtcctcg acggacagga acaccgggtg ctcgatgcgc
     1081 ggccgatccg gatcgttgga gaggatcggc acccggcctt cgtagtcgcc ggcggcgaga
     1141 ccggaggcgt cgaaggtgag ggggatcgac gccgactctc cggaccgcag ccggccggag
     1201 cgcggggcgg cggaaaccca gtccttctga atcagcagcc gcaccgccag ccggtcgtgc
     1261 acgtacgggg cgttgaagcc gatcgggagc ccggccgttc cggaggcgtc ctgaatgccg
     1321 atcgtcgcgg agtccatctg ttccaccatc tccaggtact gaaacaggat ttcgcccgaa
     1381 tcgaagagga ccatctggaa cgtgtacggt cctcctccgc cgtagcgcag cacggcgttg
     1441 tactgcaccg tgaaggcacc cggctcggcg agccagacga cgtcctgcag gccccagacg
     1501 aaaaggtcgt cccagtagcc ggccaccagg tttttggggg cgccggggtt tggcagcggc
     1561 tggttctcga aaggcgagtc ggcgtgcgtg aaggagagga agccgttgtc ggcgatccgg
     1621 acgctcgtga acgtctgtcc gtagaagggg aacgcgaagc cgagcgggat cgactccgac
     1681 atccggtcgt cggcgccgat gagagcgctg tgtcctgtgg cgccgatgtc ccgccaggcg
     1741 aagaccgggc cgccgggctc gtcgctgtcg atgaagacgt agccgaagcc gtcggcgccg
     1801 ccccagcgct cgaggcctgt ggccgcgcgc gcgggcgtgg gccggaggtt ggggcgcccc
     1861 aggctccagt cgagcgttcc ctcgctgctg ttccgcagcg tcaggagtga cgtggccgtg
     1921 tggccgctgc gcagagcggc ggacgaggag tcgggggtcg cttccagggc cggcggcggc
     1981 agggtggagg cggtggcgaa cccgggcggg ccggcgttgc cccattcgtc gaccgcccgg
     2041 agaccgaggt aatacgtgcg cgccggctgg agcccgaaca cctcctgcgt ctgcgacgat
     2101 cccgcgggga gcgggggacc ggggaccggg aagcgcgggg cgccctcgaa cccggcgacg
     2161 tcgaacggct cttccgaaac cttcaaatcg taggtggcgg cggcgccgcc gccgctgtcg
     2221 tcgccggtgt cggtccaggc gagcaccacg ccgtcgctga gcggctccac cacccggagg
     2281 tccgcgatgg ggccgggcgg cgtggtgtcg gcgtccagca ggcaccggag ggcgttcacc
     2341 ctcccgccga cggcgacgag tccggcgagc gctgcgaccg gctccgcatg ctcgaggatc
     2401 cggcggcgca gcgggcccgc ccccataccc ggcgcgaacc cgaggatgag cgccgccacg
     2461 cccgagacat gcggcgtcgc catcgaagtg ccgctgctct ggccgtagaa gccgccgggg
     2521 agcgcgctca tgatctgcac cccgggcgca gcgatgtcca cggtcgtggc gccgtggttc
     2581 gagaacggcg ccaggcgatc gtggcggtcg gtggccgcga ccgcgatgac gttgggggtg
     2641 tcgaacccgg ccgggaaggc cggcacgatg tccgaatcgt gcccgtcgtt gcccgccgcg
     2701 gcgacgaaca gcacttcgtg caggccggcg tcgcggatcg actccagcat cgccatcgag
     2761 aagccaccgc cgccccagga agcgttgatg atccgcgccc ccatgcgggc ggcgtagtcg
     2821 attgcctgga tggcgcctgc ggtgtcgccg gccccggtcg cgtccaggaa cttcaccggc
     2881 atcaggctgg cccgccaggc cacgccggcg atcccgaggc cgttgtcgcc cgcggcggcg
     2941 acaatcccgg cgacatgcgt cccgtggccg ttgtcgtcgc gcggctcgtc atcgtggttg
     3001 acgaagtccc agccgtgaac gtcatccacc agcccgttgc cgtcgtcgtc gacgaggttc
     3061 ccggcgatct cgtccgggtt ggtccagagg ttgggcgcga gatccgggtg gtcgaggtcg
     3121 atgccgctgt ccatgatcgc caccacgacc aacgcccccc cgatcgagac gtcccacgcc
     3181 ccggtggcgt cgatgtcgga gcccgcggag cctccggcct ggccggtgtt cagcagggcg
     3241 tactgttgct ggaagagcgg atcgtccggg gtccgggtcg cgtacaggag atagttcggc
     3301 tcggcgtact cgacgtcggg agcgttctcg aggcgcacca gcgcctcggg gaccgtgacg
     3361 ccggcgccga ggcgccacag ctcggcgccg gacgcgaacc ggcgctgcag ggcaccggcc
     3421 acctcgccgc gcacgacgcc acgcgacgcg gcaagtgcct cgcgcctgaa cttgacgagg
     3481 atctctcctt cgacgtactg cggaccgcgg gagtcgcgcg gggcggaggt ggggagcttc
     3541 ggagcggcag gcgaggcggc gccggcggcc agggcgaggg agaccagaag ggcggccgcc
     3601 gcatgtcgag cgcacccggg ccttgacggt cgacgcattc ccgaaccctc gcgagcgagc
     3661 cgaacctgac gtcacgatag tgagaagcac gatgcgcgat cacgacgtgc ctcggggtga
     3721 tgcggcgcat gcaccgcgcg ggtggggtca gcggcggcgg acgccggctc ggggccgatc
     3781 gtcgcgcttc gagcggctgg ccctgtccgg gcgatcggtc gggacggtga cggtcgcggt
     3841 cccggtcgcg gcacggccgg acgaatcggt cgcgacgtac gtgagcgtgt agacgcggcc
     3901 cggtccggcg acgttgcgct cgacgcgcag gagcaccgat ccgtcggcgg tccccggctc
     3961 gaggccggcg atgtcgcccg tggtgctccc gtccttccca ccgcgggcgt catcaggctc
     4021 gctgctcgcc ccggcggtca cgagcacttc gacgtctctc gagcaggcgt ccaccgcgct
     4081 ccagaacacg ttcaccggga ccatgcgatg atcgggcggc cacagcacca ggggggcggc
     4141 ggtgacgctc agcgccggcg gggtcgtatc gtccacgacg atcgtgctct cgatggtgtc
     4201 ggtcgagccc aggctgtcgg tcacgcgcaa cgccacgcgg tgcgacccga gcggcagggt
     4261 ggcggtgatg cgcgggccgg tgccaagcag cgactccgag gccccgcccg cgttctcgaa
     4321 ccattcgtac aggacgatgt cggacggcgt tccggcaggg gagtcgtcgt cgctcgacgg
     4381 ggacgcgtcg aggaccaccg ccgagcctcc cggaccggcg cattcgaccg gcgcctgcgg
     4441 ccacagcgcc ccggcctgcg gcgggacgtt gaggaccagc cagcgctccc cgccgtacag
     4501 gaagggggcg gaggcgcgca ccggccgcgt gttcccgtcc gtggcctgga ccaccacgac
     4561 gtagttcgag ccgggctcga ggtcggcgat ctccatgcga cgcgccagcg gggaagcgtc
     4621 cggagtcgca cgcaccaggg ccgcagtggt gagaaggcgg gcgaccgact ggccgtacgc
     4681 gtcgatcgtc acctcgaagt cgtccgaaga cccatcggca cgccggatgc agacggtggc
     4741 cggggcggga ggcatatcga aatacacatc gaacggagcg gtcggctcgc tgcaggggcc
     4801 ggggcccaaa aggtagtcgg ccgctccgtc gctgcagccg agcccggtgt cgagatcgaa
     4861 cagggccgcg tagccgaatc ccgcgatgta gccgatcccc tccccggtcc gcccctcggg
     4921 gagccacccc agggcgcagg agagggtggt ggccaggttc tcgatctcga acgtggcgtc
     4981 ctgcctcaag agaaggatct tcccggccag cgtctcattc tgcggatccc gggcgcggaa
     5041 gcggacctcc aggaattcgc cgccgtcctc gacgacggcg tcgatgccga tcgtcggctg
     5101 gcaggcgtcc ccggagccat ccgcgtcgga gtcggtctga tcggcgttcg cgaccgcggc
     5161 gcagttgtcg caggcatccc ccaggcggtc cccgtcggcg tccgtctggg ccggatccgg
     5221 ggtcacggga caggtgtcgc aggcgtcgcc cgcccggtcg ccatcctggt cctcctgcac
     5281 cggatcggcg tggaccggac agacgtcgca ggcgtccccc gtgccgtcgc cgtccccatc
     5341 ctgctgggcg ggattgggag tcgacgggca gttgtcggaa caggcgccat ccccgtcgag
     5401 gtccggacgg tagt
//
LOCUS       DASRIQ010000157         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_835049, whole genome shotgun sequence.
ACCESSION   DASRIQ010000157 DASRIQ010000000
VERSION     DASRIQ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835049"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFD06_03800"
     CDS             <1..191
                     /locus_tag="VFD06_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02694.1"
                     /translation="PNAVPNEASKPAVSIGAGSESTPTAPMSQSSQAGARGYLNEVRT
                     LSASTPTRDGNDESGLKS"
     gene            229..1092
                     /locus_tag="VFD06_03805"
     CDS             229..1092
                     /locus_tag="VFD06_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN02695.1"
                     /translation="MTAYRFCRTDDMGRLVEAHNHCWLPDHPADPPLTLAEFKGLVRE
                     RQLWCSSCLIAYEGQEPVGFVFGCKREKETLLHRFAVHPSHRRRGHGRHLLTSISSKA
                     AILGPPRLVVEVPEGEDAALGILRSCGYAQESILTDWRRAWPDGVLDERGEGLIIPVT
                     AAELQANGLLSDLSPSPAWERAAPSLLALGERLSGTALVVEERLAASILYMRPGAGGE
                     GRILLLAAADPASGGAQLELLLRGLLRQHPETWVFPRALPSEPAVEALRRVGFAPGAR
                     FQRMAAVAQPA"
     gene            1128..2075
                     /locus_tag="VFD06_03810"
     CDS             1128..2075
                     /locus_tag="VFD06_03810"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HZN02696.1"
                     /translation="MKGYHDIAGDGGSNVLQQVIDLEGRIAANLAGVRHLVAVGSGKG
                     GVGKSTLTFHLAALCRQAGWEVALLDADLNGPTQARMAGVAGTVPLPSDGGRLALPRA
                     RGGFGVFSVGSLVPEAEAVDFDSVSRGASHTWRATREFTLLSEILERAEWGRLDALFV
                     DLPPGAERVVQFAELLGPRASFVLISIPSDVARGVVARSAAALRRAGRRIVGRIENMQ
                     GYACSGCGQIRPLFRTTEEPGTHGGAPASGPAAAGATTVAAPALDLPLLGSVPFDPEL
                     ADLCDRGIPITERPDLPSAPALEETARALRRAFEEDRRS"
BASE COUNT          269 a          818 c          809 g          320 t
ORIGIN      
        1 gtccgaacgc ggtcccgaac gaggcctcga agccggcggt cagcatcggc gccggctcgg
       61 aatcgacgcc gaccgccccg atgtcccagt cctcccaggc gggcgcccgt gggtacctca
      121 acgaggtccg gaccctgtcc gcctcgacgc cgacgcgcga cgggaacgac gagagcgggc
      181 tgaagtcgta gccgccgttc ggacgcctcc ctctccggag ggcgcgggat gactgcctat
      241 cgcttctgcc gcaccgacga catggggcgg ctggtggagg cgcacaacca ctgctggctg
      301 ccggaccacc cggccgatcc gccgctcacg ctcgcggagt tcaaggggct ggtgcgcgaa
      361 cggcagctct ggtgcagctc ctgcctgatt gcctacgagg ggcaggagcc ggtcggcttc
      421 gtcttcggat gcaagcggga gaaggagacg cttctccatc gcttcgccgt ccacccgtcg
      481 caccgccggc gcgggcacgg gcggcacctc ctgacctcga tcagcagcaa ggcggcgatc
      541 ctcggcccgc cgcgcctcgt cgtcgaggtg cccgaaggcg aggatgccgc gctcgggatc
      601 cttcgctcct gcggctacgc gcaggagtcg atcctgaccg actggcggcg cgcgtggccc
      661 gacggcgtcc tcgacgagcg gggggaaggg ctcatcatcc ccgtcaccgc ggcggagctg
      721 caggcgaacg ggctgctgtc cgacctgtca ccctcccctg cctgggaacg cgccgccccg
      781 tccctcctgg cgctcgggga gcggctctcg gggaccgccc tggtcgtcga agagcgcctc
      841 gccgcctcta tcctctatat gcgccccgga gcgggggggg aggggcggat cctgcttctg
      901 gccgcggccg acccggccag tggcggagcg cagctcgaac tgctcctgcg cggcctgctg
      961 cggcagcacc cggagacatg ggtgttccca cgggccctcc cctccgagcc cgccgtcgaa
     1021 gcgttgcggc gcgtcggctt cgccccgggg gcccgcttcc agcggatggc cgcggtggcg
     1081 cagccggctt gaggtaggct atgcgctccg cgaggccagg ctccgagatg aagggttacc
     1141 acgacatcgc cggggacggc ggctcgaacg tcctccagca ggtcatcgac ctggaggggc
     1201 gcattgccgc caacctcgcc ggcgtgcggc acctcgtcgc cgtcggatcc gggaaggggg
     1261 gcgtcggcaa gagcaccctg accttccacc tggcggccct ctgccgccag gccggctggg
     1321 aggtggcgct cctcgacgcc gacctcaacg gaccgacgca ggcgcggatg gcgggcgtcg
     1381 ccggaaccgt gccgctgccg tcggacggcg gccgcctggc cctgccgcgg gcgcgcggcg
     1441 gcttcggcgt cttctcggtc gggtcgctgg tgcctgaggc ggaggcggtc gacttcgaca
     1501 gcgtcagccg cggcgcctcg cacacctggc gggcgacgcg cgagttcact ctcctctcgg
     1561 agatcctcga gcgggccgag tggggccggc tcgatgccct cttcgtcgat ctcccgcccg
     1621 gggcggagcg tgtggtgcag ttcgcggagc tgctcggccc gcgcgcgtcg ttcgtgctca
     1681 tcagcatccc gtcggacgtg gcgcgcgggg tcgtcgctcg ctcggccgcg gcgctgcggc
     1741 gcgccggccg tcgcatcgtg ggccggatcg agaacatgca gggatacgcc tgctccggct
     1801 gcggccagat ccggccgctg ttccggacga cggaggaacc cgggacgcac ggcggcgcgc
     1861 cggcgtccgg accggccgcc gcgggtgcca cgaccgttgc ggccccggcg ctcgacctgc
     1921 cgcttctcgg gtcggtgccg ttcgatccgg agctggcgga cctgtgcgac cgcgggatcc
     1981 cgatcaccga gcgtcccgat ctgccttcgg cccccgcgct cgaggagacc gcccgggcgc
     2041 tgaggcgtgc cttcgaggag gaccgccgct cgtgaagttc ctctgcgtcc cctgcgatgc
     2101 gccgatgaag ctggcggagg tcgccccgcc cgaccgcggc tccctctcgg tgcgctacgc
     2161 ctgtcccgag tgcggctacg agatggcgat gctgacgaac ccgttcgaga cccaga
//
LOCUS       DASRIQ010000158         4638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1367366, whole genome shotgun sequence.
ACCESSION   DASRIQ010000158 DASRIQ010000000
VERSION     DASRIQ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367366"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(679..3600)
                     /locus_tag="VFD06_03815"
     CDS             complement(679..3600)
                     /locus_tag="VFD06_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZN02697.1"
                     /translation="MAKRTRAVTGAVEEPARGVQGHDADPLSLDPRDVAADLRSRVEG
                     ELLFSPVDRILYSTAACIYQVEPLGAVRPRHEADVLAVLEYARKHRLPVTPRGGGSGL
                     TGATLGRGIVLDLSKHFRRVREIDPERGTVRAEPGVIQADLNHLLLPHGVHFAPDPSS
                     SAWCTVGGMVANNAGGSHTIGHGATRDNLVSARLALASGEVIETGTGGSGDAALRLAR
                     GVAEIAARGRDAIERHQPRTRRNSSGYALREAVRGETVDLGPLVCGSEGTLGIVLDAT
                     LRTIPIPKVKATALVLFDDLEKAGAAVVEILRHRPAAVELLDRIFVDIVRQADPATGA
                     TFPAGTEAILIVEVDGDEEREVRARLDAIAAALVDRLRLATEVRKATRPEETARVWAV
                     RKAASPILSRREGRLRNTRFIEDAAVHPERMAEFVRDLRALLRRHGLQAAIFGHAGDC
                     NLHCNPLMNQRDPAHLALMETIAAEFTDLVIGLGGSLSGEHGDGRLRTPYLARAYGPL
                     AAVFAEVKRLFDPDGILNPGIIVHDGAARLTDDLRYGAEYARVATTTTLDDEAWQREI
                     EKCHGCGACRHYCPVAVETGDEAATARAKGNLLRAALSGRLPATALATDAFKEVMDLC
                     VNCRLCHSECPTAIDIPGMAILAKEAYVRARGKRHADRVLTASGPLLRLGTATASIAN
                     AAMANPALRRLMAPLTGLSAERRMQAFDPAPLQTRMPSVDPDGDKVAYFHGCFGGYQD
                     VEGEGRAAIDLLEAIGCTVAIPPQECCGIAAITYGHLDDVRPAAARNVATFMDLLRRG
                     YTPLYSAPSCGLALVEDAPRLLGTPQSEVLARHLRDVGDYVLERLEARPELRARLRPV
                     PLRLTYHNPCHSQARGLGDSVLRLLRLVPGVEVVPIVKDHCCGIAGTFGMKACNFDQS
                     MRMGRPLFESITATGAPVVATGCGTCKIQIEQASGLPVVHPIKVVRDSLLGMVSAVSP
                     R"
     gene            complement(3717..4199)
                     /locus_tag="VFD06_03820"
                     /pseudo
     CDS             complement(3717..4199)
                     /locus_tag="VFD06_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
     gene            complement(4251..>4638)
                     /locus_tag="VFD06_03825"
     CDS             complement(4251..>4638)
                     /locus_tag="VFD06_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZN02698.1"
                     /translation="VDLATLTGACVVALGPMASGLMSNDQRLADDLLRAAGAAGEAVW
                     QLPLYQEYREHIKSDVADIKNTGIRWGGAITAALFLQEFVRDGLPWAHMDIAGPAFGE
                     KEYSYLGKGGSGAGVRTLLRWLAPPP"
BASE COUNT          640 a         1692 c         1630 g          676 t
ORIGIN      
        1 ccatcgggcc cggattgaaa gagcgttcgt tctacacatt ctggacccca tgatttcaag
       61 tttgagttgg ttgctttcgc aatgtccacg agaccgtcta cccgcaccct ccagtttcat
      121 gccggtccct ccccttcgcg aaccgccggc gacgacgcac gcgctcgacg accttcgggg
      181 cggatgcaag tagcgagggg cgatccggcg ggaccttccg gcggaagtcc ggcgcggggc
      241 tgtggagcca gcgtcggatc cgatggggct tgagagaggc gtgtcggagc catcgcgcga
      301 ccgtgctctt gtgggcgcct aatgcatact gcccacactc tacgggtcaa gagctagcga
      361 acctcttgta cgccggcgag aaatcgctgt caacgggttg gtgttgggaa cggcggaagc
      421 tggtcggatg gcgtgatcac ctgatactgc tctagtggtg gggcctatcc gtttcaggac
      481 tgtcaacggt tttcggccca gggagtctgg gttcgacagg gcggctggct caggtgcggc
      541 gattccgtca ggtgctggcc cacggcacca tcgtcgagca gaaggtgtta ctgcgcggct
      601 tcatcgcagg gatcacgatc accccgagca aggacaccgg cgtcatcacc tggttcgacc
      661 tgccagcatc tcttgagttc agcgggggga cacagcagaa accatcccga gcagcgagtc
      721 cctcacgacc ttgatcgggt gcacgaccgg gagtcccgac gcctgctcga tttggatctt
      781 gcaggtgccg cagccggtgg cgacgaccgg ggcgcctgtg gcggtgatgc tttcaaacag
      841 cggtcgcccc atccgcatcg actgatcgaa gttgcacgcc ttcatcccga aggtgccggc
      901 gatgccgcag cagtggtcct tcacgatcgg caccacctcg acgccgggga ccagccgcag
      961 cagacggagc accgagtcgc cgagtccgcg cgcctgggaa tgacacgggt tgtgatacgt
     1021 cagacgcagc ggcaccggcc gcagccgcgc ccgcagctcg ggacgcgcct ccaggcgctc
     1081 gaggacgtag tcgccgacgt cgcgcaggtg ccgcgccagg acctccgatt gcggcgtccc
     1141 caggagacgc ggcgcgtcct cgacgagcgc caggccgcag gatggggcac tgtacagagg
     1201 cgtgtagccg cggcgcagga gatccatgaa cgtcgcgacg ttgcgcgccg ccgccgggcg
     1261 cacgtcgtcg aggtggccgt aggtgatggc ggcgatgccg cagcattcct gcggcgggat
     1321 cgccacggtg cagccgatcg cttcgagcag atcgatcgcc gcgcgcccct ccccttcgac
     1381 atcctggtag ccgccgaagc agccgtggaa gtaggccacc ttgtcgccgt ccggatcgac
     1441 cgacggcatc ctcgtctgca gcggcgccgg gtcgaacgcc tgcatccggc gctcggcgct
     1501 gaggccggtc aaaggggcca tcagccgccg cagcgccggg ttcgccatgg cggcgttggc
     1561 gatcgacgcc gtcgccgtcc ccagccgcag cagcggcccg gaggcggtga gcacccgatc
     1621 ggcgtgccgc ttcccgcgcg cccgcacgta cgcctccttc gccaggatcg ccatcccggg
     1681 gatgtcgatc gccgtcgggc actcggagtg gcacagccgg cagttgacgc agaggtccat
     1741 gacctccttg aacgcgtcgg tcgcgagtgc tgttgccggc aggcgtccgg agagcgccgc
     1801 cctgaggaga tttcccttgg cgcgcgccgt cgccgcttcg tcaccggtct cgacggcgac
     1861 cgggcagtag tgccggcagg ctccgcagcc gtggcacttc tcgatctccc gctgccacgc
     1921 ctcgtcgtcg agcgtggtcg tcgtcgcgac ccgggcgtac tccgcgccgt aacgcaggtc
     1981 gtcggtcagc cgcgcggcgc cgtcgtggac gatgatcccc gggttgagga tcccgtccgg
     2041 atcgaagagc cgtttcacct cggcgaagac cgccgcgagc gggccgtagg cgcgcgcgag
     2101 atacggcgtg cgcagccggc cgtcgccgtg ctcccccgac agcgatccgc cgagcccgat
     2161 gacgaggtcg gtgaactcgg ccgcgatcgt ctccatcagc gccaggtgcg ccgggtcgcg
     2221 ctggttcatg agcggattgc agtgcaggtt gcagtcgccg gcgtgcccga agatcgccgc
     2281 ctgcagcccg tggcggcgga gcagcgcccg gagatcccgg acgaactcgg ccatccgctc
     2341 cgggtgcacc gccgcgtcct cgatgaaccg ggtgttgcgc agccgcccct cgcggcgcga
     2401 caggatgggc gaggccgcct tgcgcaccgc ccagacgcgc gccgtctcct ccggccgcgt
     2461 cgccttgcgc acctcggtcg cgaggcgtag ccggtcgacg agcgccgccg cgatcgcgtc
     2521 gaggcgcgcg cgcacctccc gctcctcgtc gccgtccacc tcgacgatca ggatcgcctc
     2581 ggtcccggcc gggaaggtgg cgcccgtggc cgggtccgcc tgccgcacga tgtccacgaa
     2641 gatccgatcg aggagctcga ccgcggccgg ccggtggcgc aggatctcca cgaccgccgc
     2701 gcccgccttc tcgaggtcgt cgaacagcac gagggcggtc gccttcacct tcgggatcgg
     2761 gatggtccgg agcgtcgcgt cgaggacgat cccgagcgtc ccctccgagc cgcagacgag
     2821 cggcccgaga tcgacggtct ccccgcgcac cgcttcgcgc agcgcgtagc cggaggagtt
     2881 gcgccgggtt ctcggctggt ggcgctcgat ggcgtcgcgt ccgcgggcgg cgatctccgc
     2941 cacgccgcgc gccagccgca gcgccgcgtc accggatccc cccgtcccgg tctcgatcac
     3001 ctcgccgctc gcgagggcga ggcgcgccga cacgaggttg tcgcgggtgg cgccgtggcc
     3061 gatcgtgtgc gaccctcccg cgttgttggc caccatgccg ccgaccgtgc accaggcgga
     3121 cgaggagggg tcgggggcga agtgcacgcc gtgcggcagg aggaggtggt tcaggtcggc
     3181 ctggatcacc cccggctcgg cgcgcaccgt gccgcgctcc ggatcgatct cccgcacgcg
     3241 gcggaagtgc ttcgacaggt cgaggacgat gccgcgcccg agggtcgcgc cggtgaggcc
     3301 ggagccgccg ccgcgcggtg tgaccggcag gcggtgcttt cgcgcgtact ccaggaccgc
     3361 caggacgtcc gcctcgtggc gcggccggac cgcgccgagc ggctccacct ggtagatgca
     3421 ggcggcggtg ctgtagagaa tccggtcgac cggcgagaac agcagctctc cttcgacgcg
     3481 ggaacgcaga tcggccgcca cgtcccgcgg atccagtgaa agcggatccg cgtcgtgtcc
     3541 ctggacgccg cgtgcgggtt cctccaccgc accggtcacc gcccgcgttc gcttcgccat
     3601 caccgcccgc tccgtcctct tccagcagtt ttgcgccggg cagcgccgcg ccgtcctccg
     3661 ccgcgccggg ccgccgcgtg ctccccgccg cgcggcccgt gcaggagccc ggcggcatgt
     3721 cccagctccg gcacgatcag ccgtcgcgcc gccagccgga ccgcggccgg cgaacccggc
     3781 atgacgaaca ggagccgtcc ccgccaggtc ccggccacgg cgcggctcag catggcggcg
     3841 gcgccgatct cacgataact cagcatccgg aagagctccc cgaatccgtc cagccgcttc
     3901 tcgaggagac ccgcgaccgc ctcgtaggtc cggtcgcgcg gggcgatgcc ggtcccccct
     3961 gtgatcagga tcgcccgcac cggcgtcccc gcgcgggcga agcgccggag ctgcgcccgc
     4021 acttcgcgcg gctcgtcccg cacgatcgcc gtctccacca ccgggtggcc ggcgcgccgg
     4081 agcgccgcgc gcagccacgc accgctccga tcggtcccgg gccggcgggt gtcgctgacc
     4141 gtgaggatgg cacaggggac cgcgacggga cccgcgcgtc ggtgctgccg gtggctcatg
     4201 atggtcgtgg cccggggccg ctcggtgcga gggcggggcg ggccggcccc tcagggcgga
     4261 ggggcgagcc agcgaaggag ggtgcgcacg ccggcgccgg agcccccctt gcccaggtac
     4321 gaatactcct tctccccgaa cgccggcccg gcgatgtcca tgtgcgccca cggcaggccg
     4381 tcgcgcacga actcctgcag gaacagggcc gcggtgatcg ccccccccca gcggatgccg
     4441 gtgttcttga tgtccgccac gtcgctcttg atgtgctcgc ggtattcctg gtagagcggc
     4501 agttgccaga ccgcctcccc cgccgcgccg gccgcgcgca gcaggtcgtc cgcgagccgc
     4561 tgatcgttgc tcatcagccc cgacgccatc ggcccgagcg cgacgacgca cgcccccgtg
     4621 agcgtggcga gatcgacc
//
LOCUS       DASRIQ010000159         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_589374, whole genome shotgun sequence.
ACCESSION   DASRIQ010000159 DASRIQ010000000
VERSION     DASRIQ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589374"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VFD06_03830"
     CDS             <1..1058
                     /locus_tag="VFD06_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02699.1"
                     /translation="RHYVETTRERFDLVVYGHLDSHTALSGFTNLRLDNYIHTVEAFA
                     ACRRLLLPGGALHVSFWATQGWVAARLSENLRRAFGRPPLSYSAPEEHGTIQARFVAS
                     DDPGLLERARGMVAADAWSFEGPTPPASTDDWPFLFVERRRVPAPMLLVALPLLLLCG
                     GLVAGLSRRTRDAASSGSVPGIDRRGQAHFFLLGAAFLLVEVHNVSRLARLFGTTWTV
                     NAWVVGGVLVAILAANLLAGRVRPADGSRIVFAGLLVSLLAGAVVPPGLLGGLPGGGL
                     LAVVLFTLPILFAGIVFGWSFRAAADAPRALGANVLGSVLGGFLELAAFLAGLPGLVL
                     IAAALYAAALALSPRNP"
     gene            1147..1374
                     /locus_tag="VFD06_03835"
     CDS             1147..1374
                     /locus_tag="VFD06_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZN02700.1"
                     /translation="MLNQDVLKGKWKQMRGQVKQWWGDLTDDDLDQIDGSVDKLVGRL
                     QERYGYAKEDAEKEVDRRLKTYGTGTDPRMP"
     gene            complement(1385..2203)
                     /locus_tag="VFD06_03840"
     CDS             complement(1385..2203)
                     /locus_tag="VFD06_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02701.1"
                     /translation="MTCTLSRSRATGRARTSALVALLFVPALLLGCGLIDPVKKVSEN
                     AEVRAQVIEALVSDPGRRNEVIARFMAQPADRKALLDQLLGDETAKGALVETILADDR
                     GKALVAAKVAANDAGAKTFIRMLMTTGVMGASLTQKQADALGYGDAYSYGTRRRTMAD
                     LKSLGRAVDSWAKEHDGAYPVCNGLDDVRTCLGRSLEKERVASLRLDDAWGKPLMYWS
                     DKEGKEYLLISYSTDGAWDGAGRTGPTDGLDCDIVFSNGDFVQWPGSFRKEEIQ"
     gene            complement(2432..3670)
                     /locus_tag="VFD06_03845"
     CDS             complement(2432..3670)
                     /locus_tag="VFD06_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN02702.1"
                     /translation="MKPPEPPVPPRIDHAAIRRERLRTLLPRLMEEQQVEAWLTFTRE
                     NVPDPLLPVFGIGHIVARAAFLFTRTDGRFRATALAASYDVDPIARTGLFDEVLPYKE
                     EGAKPHLRRLIAEGGLTRVAVNVSRDVTIADGLTSGMRRYLEETLGPEIARGFTSSER
                     LVVSLLGRKLPAEIAALETAVLATQRICAEALTRERVVPGTTTERALADFMAARAREL
                     GCAMAFESVVVGPARGHSEPTDRVIREGDVIRLDWGASYEGYCADIQRTAWVAKAGQP
                     AVPAWLARLFAATLKANRAALAALRPGNTGLDVDRAGRSSLVADGYAEYPHGSGHAIG
                     LKVHDVGPLLGPDWKERYGDQVRFRIEPDQVFAVEPLLYIRPPELDYDFHVGLEEDAV
                     VGPDGARVIGAPQTEVILIA"
     gene            complement(3707..>3982)
                     /locus_tag="VFD06_03850"
     CDS             complement(3707..>3982)
                     /locus_tag="VFD06_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02703.1"
                     /translation="GRRAVIAHRRIRKGEPLTFFYPSTEWRMARPFPCHCGAPGGLAE
                     VRGARDLPLAVLRRFPLSAHIRRMKEAQARARTGRRRARARIRETAA"
BASE COUNT          542 a         1368 c         1457 g          615 t
ORIGIN      
        1 cgcgtcatta cgtcgagacg accagggaac gcttcgacct cgtcgtgtac ggccaccttg
       61 actcgcacac cgctctgtcc ggcttcacga acttgagact ggacaactac atccacacgg
      121 tcgaggcctt cgccgcgtgc cgccggctac tccttcccgg cggcgcgctg cacgtgtcgt
      181 tctgggcgac gcaggggtgg gtcgccgcgc gcctctcgga gaatctccgg cgcgccttcg
      241 ggcgaccgcc gctgtcctac tccgctcccg aggagcacgg cacgatccag gcgcgcttcg
      301 tggcgagcga cgaccccggt ctcctcgagc gagcgcgtgg gatggtggcc gcggacgcct
      361 ggagtttcga aggcccgact cctcctgcgt cgaccgacga ctggccgttt ctgttcgtgg
      421 agaggcggcg ggtgccggcg ccgatgctcc tcgtcgcact gccgctcctg cttctgtgcg
      481 gcggtctggt cgccggcctg tcgcgccgaa cgcgcgacgc tgcctcctcc ggctccgtcc
      541 cgggcatcga ccgaaggggg caggcgcatt tcttcctcct gggggccgcg ttcctgctgg
      601 tcgaggtgca taacgtcagc cggctggcgc ggctgttcgg cacgacctgg acggtgaacg
      661 catgggtcgt cggcggggtg ctcgtcgcca tcctggcggc gaatctcctg gcgggtcgcg
      721 ttcgtccggc ggacgggagc cggatcgtct tcgcggggct cctggtgagc cttctggccg
      781 gggcggtcgt gccgccgggt ctgctggggg ggctgccggg cggcggcctg ctggcggtcg
      841 tcctcttcac gctgccgatc ctgttcgcgg ggatcgtctt cgggtggtcg ttccgcgccg
      901 ccgccgacgc gccgcgcgcc ctcggcgcga acgtgctcgg ctcggtgctg ggaggcttcc
      961 tcgagctcgc ggcgtttctc gccggcctgc cggggctcgt cctgatcgcg gcggccctct
     1021 acgccgcggc cctggcgctc tctccgagga atccctgacg gaagaatccc cgaccccgga
     1081 atgcgtggtc tgccgaatac ctgccggcgc gcccaggccc tatcctcgtt cctgaggagg
     1141 gcagcgatgc tcaaccagga cgttctcaag ggaaaatgga agcagatgcg gggtcaggtc
     1201 aagcagtggt ggggcgacct gacggacgac gatctggacc agatcgacgg gagcgtggac
     1261 aagctcgtcg gccgcctgca ggagcgctac gggtatgcca aggaggacgc cgagaaggaa
     1321 gtcgatcggc gcctcaagac gtacggcacc gggaccgatc cgaggatgcc gtaaacgaac
     1381 gcggttactg aatctcttct ttgcggaacg accccggcca ctgcacaaag tctccgttcg
     1441 agaagacgat gtcgcagtcg agtccgtcgg tgggaccggt gcggccggca ccgtcccacg
     1501 ctccgtcggt cgagtatgaa atcagcagat attccttccc ttccttgtcc gaccagtaca
     1561 tcagcggctt cccccaggcg tcgtcgagac gcagcgacgc gacccgctcc ttctccaggg
     1621 agcggccgag gcaggtgcgc acgtcgtcca gcccgttgca cacgggatag gcgccgtcgt
     1681 gctccttcgc ccaggaatcc acggcccggc ccagagactt gaggtcggcc attgtgcgcc
     1741 ggcgcgtgcc gtaggagtag gcgtcgccgt atccgagcgc gtcggcctgc ttctgggtca
     1801 gcgacgcccc catgacgccg gtcgtcatca gcatgcggat gaacgtcttc gcccccgcgt
     1861 cgttcgccgc caccttcgcg gcgaccagcg ccttgccccg atcgtcggcg aggatggtct
     1921 cgacgagcgc ccctttggcc gtttcgtccc ccagcagctg gtcgagcagg gccttgcggt
     1981 cggccggctg cgccatgaag cgggcgatca cctcgttgcg tcgtccaggg tccgagacca
     2041 gcgcctcgat cacctgcgcc cgcacctcgg cgttctccga cactttcttc accgggtcga
     2101 tgaggccgca cccgagcagg agggccggga cgaagagcag cgcgacgagg gcggacgtgc
     2161 gggcgcgccc ggtggcgcgg ctgcgtgaga gggtgcaggt catcggggat cctccggtcg
     2221 aaggtttccg agtcggcgag acgacggcgc gccggagtct agcaaatcac gctctcaagg
     2281 acagccgtcg tcgcaggagt cggggagccg cggccagggg gccgattcgg tcgaatagcc
     2341 ggtcgtgacc cccgcggcga tcgtctggat gaggtctcga tgagttcgag ccggctggag
     2401 aggtcacaat ttcacgcccg acgcgggggg atcaggcgat gaggatgacc tcggtctgcg
     2461 gcgcgccgat gacccgcgcg ccgtcgggac ccacgacggc gtcctcctcc aggccgacgt
     2521 ggaagtcgta gtcgagctcc ggcggccgga tgtaaagcag cggctccacg gcgaagacct
     2581 ggtccggctc gatccggaag cgcacctggt cgccgtagcg ctccttccag tcggggccga
     2641 ggagcggccc gacgtcgtgc accttcaatc cgatggcgtg gccgctgccg tgcgggtact
     2701 cggcgtagcc gtcggccacg agcgaggacc gcccggctcg atcgacgtcc agtccggtgt
     2761 tccccggccg cagcgccgcc agggcggccc gattggcctt gagggtcgcg gcgaacagcc
     2821 gcgcgagcca ggcggggacg gccggctggc ccgccttggc tacccaggcg gtgcgctgga
     2881 tgtcggcgca gtacccctcg tatgaggccc cccagtcgag ccggatgacg tccccttccc
     2941 ggatgacccg gtcggtcggc tcggagtggc cgcgcgccgg cccgacgacc accgactcga
     3001 acgccatggc gcagccgagc tcgcgggccc gcgccgccat gaagtcggcg agggcgcgct
     3061 cggtcgtggt gccgggcacg acccgctcgc gcgtcagcgc ctcggcgcag atccgctggg
     3121 tcgccaggac cgccgtctcg agcgcggcga tctcggccgg gagcttgcgg ccgaggagcg
     3181 acaccaccag gcgctcggag gaggtgaagc cgcgggcgat ctccgggccg agcgtctcct
     3241 ccaggtagcg ccgcatcccg gaggtcagcc cgtcggcgat ggtgacgtcg cgcgagacgt
     3301 tgacggcgac gcgggtcagc cccccctcgg cgatcagccg gcggaggtgc ggcttggccc
     3361 cttcctcttt ataggggagg acctcgtcga acaggccagt ccgggcgatc gggtcgacgt
     3421 cgtaggaggc ggcgagcgcg gtcgcccgga agcgcccgtc ggtccgggtg aacaggaagg
     3481 cggcgcgcgc cacgatgtgg ccgatgccga acaccggcag aagagggtcc gggacgttct
     3541 cgcgcgtgaa ggtgagccag gcctcgacct gctgttcctc catcagccgc gggagaaggg
     3601 tgcggaggcg ctcgcggcgg atggccgcgt ggtcgatccg cggcggcacg gggggctccg
     3661 gcggtttcat cgcggggtca ttctaaccgg ggacagcagg aggcgttcag gcggcggtct
     3721 cacggatgcg cgcccgcgcg cgccgccgac ccgtccgggc gcgcgcctgc gcctccttca
     3781 tgcgccggat gtgggcgctc agggggaacc gccgcagcac cgcgagcggc aggtcgcggg
     3841 cgccgcgcac ctcggcgagg ccgcccgggg cgccgcagtg gcaggggaag ggccgggcca
     3901 tccgccattc ggtcgagggg tagaagaagg tcagaggctc ccccttgcgg atgcggcggt
     3961 gggcgatcac ggcgcgccgg cc
//
LOCUS       DASRIQ010000160         6544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_836222, whole genome shotgun sequence.
ACCESSION   DASRIQ010000160 DASRIQ010000000
VERSION     DASRIQ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836222"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4859
                     /locus_tag="VFD06_03855"
     CDS             <1..4859
                     /locus_tag="VFD06_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN02704.1"
                     /translation="TDYPGGGREIRDLNRNLQIDTPDVTDPFIGIQLPDEDVTFNSMF
                     IVGTANGFVSNTLGEDLNNDGDNTDPGEGDFIPNLVLDKGILALPGGPSANDKVPFSL
                     DTNNGGFLGLRHPFSRPGTSAASVVWEWQRGGLCGFQSAIPDGNPTKGFANNGAGIWH
                     TGDGNIATPDTNAAGCDNHSIATDPVTPVGTEFYMDILESPIVAKVHQTLDSRNLPYA
                     VEFQRFAANLDHQTRDGNTGGNFNIDNNIEDDTGNCLLCQEFDPNYGGIDYQVGNLQA
                     SSGVDPTNSGDIRQRTFGPRLDPDGSTTGGNPQVSGDETGFSGFTTSANPNSASPIPT
                     AFPDFLPYPLPGAPVVQASDGTPWTNNVQGPVRNFDMTLVTYEAGYVFLMAGPGASEV
                     AAVTPFDVNPGNRWQIGIGFYNIESAGNLNDYGLAVDDVVFEWDERHPVDEGAFIPAH
                     TPACQRFGQPNQPAGQQCATLMVDRTSLFECDESLRITVHNPKVSGSGSVQVSAASDS
                     DSRQVSTGIGVALHPIKTFTLPEVSPGLFSGFVAVTQTLNVDDQLFVSAAGDSNIVFY
                     YQDPTCDGNRNGIAGQTNFDNLDGDNVAFASDNCPQAYNPTQTDTDTGGADGAGNACD
                     NCPTLPNANQSDSDGDGVGDVCDFDDIDFDGVVNSLDNCPDVYNVLQTPAGGSSTRGT
                     ACNQNADRDGDGLNDRLDNCVRTSNAGQQDIDGDGVGDVCDGDCVNARAQLLAGGSCS
                     RTSTTICTSNAQCPVSGICAESITTVCTVSTAQCTCVNIAQEICQRSGIVNDGGCQVE
                     DDDADADGVSDGVDNCPVISNPPIIPGTDRQADADNDGLGDICDSALKIDGDNNGIPD
                     DVASFGVLVNCGKLDLPNLVVESTTVNDLNGDGDAFCDTGEKCEMTVVVANAGTQNLT
                     DVTIYLATSDTDVQCVTKPSILVGSLPAGGKVDTSNIGGQRRVFEYTISPTTQTVVAS
                     QPAKADWTLNVSSRQALGTKGKVTFQTLLDLDLPVGAPITRVLGPDGLPNTVDDGQIV
                     ETFDVDRNGIGGVEISDGRNGVPNDTIGYTVGTALGGLNALEGIGCGGFRVPPQDPGC
                     HVDPDNDMDWHIHCPVGTCPAPNVVGSTTAFSTTPTNGAMAFSLNNSLHWGRHTDPLN
                     RSGDTVSFRTLAAFVTTANLTPLPVSGDLRLSFMHIVDTMDNTQADIPVGMAIDFVDV
                     QVRVDQDANPTTDNWGFWDKLVPFENVYDHIPYVWSHYGAQTTYCNLTPTDTGSAAPA
                     PRGTHETMCWPLGVWSHCGNAWGTDTTFGCPGPGLNGATAPQSGALWVQSRFSLANYI
                     GARVQIRWIASTWEFDLNGPTEDYHTYGHGWENSLNDDGWWVDDIVITGAITTQVSPA
                     ADPDPAPPATCPAPGSECNPALGADHGYNVVLTVTGSDNDPAFEKGENVNFSAATTTN
                     PGGCADGVTEFQFRRNGVVVQDWSARAFYDEGVQADGTWTVQARCSSNAACTTTTGAS
                     ATLPVYAGDGSDLIITLAALNTAGNANINLVARPMPPTMTGYDFFRYTIPPAPPANLA
                     GTIILFCDQGLAAPVGSTVTVNDAAIPAVGQAFLYWAGYSSPTAGAKTALGRRTDTTI
                     RIAPLTCP"
     gene            complement(4939..>6544)
                     /locus_tag="VFD06_03860"
     CDS             complement(4939..>6544)
                     /locus_tag="VFD06_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZN02705.1"
                     /translation="VDATDDLYQDDVHFMDGGIHVDSWEMSMGLANAMPGAPGYVIDD
                     RLFADRFDQRPWMFTYKKQQRDGPFWDRTALKARYESIRIPTFVIGGWYDGYRDSVPR
                     MLEHVKAPMKAIVGPWSHCFPHDAYPKPGMEWRHEAVRWFDRWLKGKETGIDREPRLA
                     VFVRRWHPPGPRLEGNAPGEWRYEDGWPLARGRELALSPRDDHTLGPAMETARLAAGS
                     VSPPAAAHRLRYVPTSGVEIGGPVMWWGDVAPDQRPSDAYSLVYDTEPLTEEVEILGL
                     PRAVLRVSADAPLARWFARLSDVAPDGTVTLVAGAGENGAHRVSAREPRAIEPGKPFT
                     LDIEMHFTSWVFAPGHRMRFAVSNAMWPMIWPTPHPMTTTLLLGGADGTRLRLPVVPK
                     AERPRPNFLPPEKNEELAEFGVLETGTASGYGEIESIERHPPTGEARITATNGGGSRF
                     PWGTEHNTESIRHEAGDAHPEAASMTGDYSTTVTLPDRTLRFEARAVFRSDAKNFYLT
                     YTRRLFEDGVLVREKTWDEPIPRDFQ"
BASE COUNT         1099 a         2382 c         2149 g          914 t
ORIGIN      
        1 ccaccgacta cccgggcggc gggcgggaga tccgcgacct gaaccgcaat ctccagatcg
       61 acacgccgga cgtcacggat ccgttcatcg gcatccagct gccggacgag gacgtcacct
      121 tcaactcgat gttcatcgta gggaccgcga acgggttcgt cagcaacacc ctcggcgagg
      181 acctgaacaa cgacggcgac aacaccgacc ccggcgaggg ggacttcatc ccgaacctcg
      241 tcctcgacaa ggggatcctg gcgctgccgg gaggcccgtc ggcgaacgac aaggtgccgt
      301 tctccctcga cacgaacaac ggcggcttcc tcggcctgcg ccatcctttc agccgccccg
      361 gcaccagcgc cgcgagcgtg gtgtgggagt ggcagcgcgg cgggctgtgc ggcttccagt
      421 cggccatccc ggacggcaac ccgaccaagg gattcgccaa caacggcgcc gggatctggc
      481 acacggggga cggcaacatc gccaccccgg atacgaacgc cgcgggctgc gacaaccact
      541 ccatcgcgac cgatccggtg acgccggtcg gcaccgagtt ctacatggac atcctggagt
      601 cgccgatcgt cgccaaggtc caccagacac tcgacagccg caacctgccg tacgccgtcg
      661 agttccagcg cttcgccgcc aacctcgatc accagacgcg tgacgggaac accggcggga
      721 acttcaacat cgacaacaac atcgaggacg acaccgggaa ctgcctgctg tgccaggagt
      781 tcgacccgaa ctacggcggc atcgactacc aggtcggcaa cctgcaggcc tcgagcggcg
      841 tcgatccgac gaactcgggg gacatccgcc agcgcacctt cggaccccgt ctggatccgg
      901 acggctcgac gaccggcgga aacccccagg tgagcggcga cgagaccggc ttctccggct
      961 tcacgacctc cgccaatccc aacagcgcct cgccgatccc gacggcgttc cccgatttcc
     1021 tgccttatcc gttgcccggg gcgccggtgg tgcaggcctc cgacggcacg ccgtggacga
     1081 acaacgtgca gggaccggtg cgcaacttcg acatgacgct cgtcacctac gaggcgggct
     1141 acgtcttcct gatggcgggg ccgggggcga gcgaggtcgc ggcggtgacg ccgttcgacg
     1201 tcaaccccgg gaaccgctgg cagatcggca tcggcttcta caacatcgag tcggccggca
     1261 acctgaacga ttacgggctg gcggtggatg acgtggtctt cgagtgggac gagcgccacc
     1321 cggtggacga gggggccttc atcccggcgc atacgcccgc gtgccagcgc ttcgggcagc
     1381 ccaaccagcc ggccgggcag cagtgcgcca ccctgatggt cgatcgcacc tcgctgttcg
     1441 agtgcgacga gtcgctccgg atcacggtgc acaatcccaa ggtgtcgggc tccggctcgg
     1501 tgcaggtgag cgccgcctcc gacagcgact cgcgccaggt gtccaccggc atcggcgtgg
     1561 cgctgcaccc gatcaagacc ttcacgctgc cggaggtgtc gcccgggctg ttcagcggct
     1621 tcgtcgccgt gacgcagacg ctgaacgtgg acgatcagct gttcgtctcg gcggcggggg
     1681 acagcaacat cgtcttctac taccaggacc cgacctgcga cgggaaccgc aacggcatcg
     1741 ccgggcagac caacttcgac aacctcgacg gcgacaacgt cgccttcgcc agcgacaact
     1801 gcccgcaggc gtacaacccg acccagaccg acaccgacac cggtggagcc gacggggcgg
     1861 gcaacgcctg cgacaactgc ccgaccctgc cgaacgccaa ccagtccgat tcggacggcg
     1921 acggtgtcgg cgacgtctgc gacttcgacg acatcgactt cgacggcgtc gtgaactcgc
     1981 tggacaactg tcccgatgtc tacaacgtcc tgcagacgcc ggcgggcggg tcctcgacgc
     2041 gcggcaccgc gtgcaaccag aacgccgacc gggacgggga cgggctcaac gatcgcctcg
     2101 acaactgcgt gcgcacctcc aacgccgggc agcaggacat cgacggcgac ggcgtcggtg
     2161 acgtctgcga cggcgactgc gtcaatgccc gggcgcagct gctggcgggc ggctcctgca
     2221 gccgcaccag cacgacgatc tgcacctcca atgcgcagtg ccccgtctcc ggcatctgcg
     2281 ccgagagcat caccacggtc tgcaccgtct cgacggcgca atgcacctgc gtcaacatcg
     2341 cgcaggagat ctgccagcgc tccggcatcg tcaacgacgg cggctgccag gtggaggacg
     2401 acgacgccga cgccgacggc gtgtccgacg gcgtcgacaa ctgcccggtc atctccaacc
     2461 cgccgatcat ccccggcacc gatcgccagg cggacgccga caacgacggc ctcggggaca
     2521 tctgcgactc ggccctcaag atcgacggcg acaacaacgg catcccggac gacgtcgcgt
     2581 cgttcggcgt gctggtgaac tgcggcaagc tggatctgcc gaacctggtc gtcgagtcga
     2641 cgacggtcaa cgacctcaac ggcgacggcg acgccttctg cgacaccggc gagaagtgcg
     2701 agatgaccgt cgtggtcgcc aacgccggga cgcagaacct caccgacgtc accatctatc
     2761 tcgccacctc cgacaccgac gtgcagtgcg tcaccaagcc gtcgatcctg gtcgggtcgc
     2821 tccccgccgg cggcaaggtg gatacctcca acatcggcgg ccagcgccgc gtcttcgagt
     2881 acaccatctc gccgacgacg cagacggtgg tggcgtcgca gccggccaag gccgactgga
     2941 ccctcaacgt gtcgtcgcgc caggcgctcg gcacgaaggg gaaggtcacc ttccagacgc
     3001 tgctcgacct cgacctgccg gtcggcgcgc cgatcacccg tgtgctgggc ccggacggcc
     3061 tccccaacac ggtggacgac ggccagatcg tcgagacctt cgacgtcgat cgcaacggca
     3121 tcggcggtgt cgagatcagc gacgggcgca acggcgtgcc caacgacacc atcggctaca
     3181 ccgtcggcac cgccctcggc gggttgaacg ccctcgaagg gatcggctgc ggcggcttcc
     3241 gcgtcccgcc gcaggacccc ggatgccacg tcgacccgga caacgacatg gactggcaca
     3301 tccactgccc cgtcggcacc tgcccggcgc cgaacgtggt cgggtccacc accgccttca
     3361 gcaccacgcc gacgaacggg gcgatggcct tctccctcaa caactcgctg cactgggggc
     3421 gccacaccga ccccctcaac cggtccggcg acaccgtctc gttccgcacc ctcgcggcct
     3481 tcgtgacgac ggccaacctg acgccgctgc cggtctccgg agatctgcgc ctttcgttca
     3541 tgcacatcgt cgacaccatg gacaacaccc aggccgacat cccggttggc atggccatcg
     3601 acttcgtgga cgtgcaggtc cgggttgatc aggatgccaa cccgacgacc gacaactggg
     3661 gcttctggga caagctggtg ccgttcgaga acgtctacga ccacatcccg tacgtctgga
     3721 gccactacgg cgcccagacg acctactgca acctgacgcc gaccgacacc ggctcggcgg
     3781 cgccggcccc gcgcggcacc cacgaaacga tgtgctggcc gctcggcgtc tggtcgcact
     3841 gcggcaacgc ctgggggacc gacaccacgt tcggctgccc cggcccggga ttgaacggcg
     3901 ccactgcgcc gcagagcggg gccctgtggg tgcagagccg cttctcgctc gccaactaca
     3961 tcggtgcccg ggtgcagatc cgctggatcg cctccacctg ggagttcgac ctgaacggcc
     4021 cgaccgagga ctaccacacc tacggccacg gctgggagaa cagcctcaac gacgacggct
     4081 ggtgggtgga cgacatcgtc atcaccggcg ccatcaccac ccaggtgtcg ccggcggccg
     4141 accccgaccc ggctccgccg gccacctgcc cggcccccgg ctcggagtgc aacccggcgc
     4201 tcggggcgga ccacggctac aacgtggtcc tgaccgtgac cggatccgac aacgacccgg
     4261 ccttcgagaa gggggagaac gtcaacttca gcgccgccac caccaccaac ccgggcggct
     4321 gcgccgatgg agtcaccgag ttccagttcc ggcgcaacgg cgtggtcgtg caggactggt
     4381 cggctcgcgc cttctacgac gagggggtgc aggcggacgg cacctggacg gtccaggccc
     4441 ggtgcagcag caacgcggcc tgcaccacga cgaccggcgc cagcgcgacc ttgccggttt
     4501 acgccggcga tggctccgac ctgatcatca ccctggcggc gctcaacacc gctgggaacg
     4561 ccaacatcaa cctcgtggcg cggccgatgc cgccgacgat gaccggctac gacttcttcc
     4621 gctacacgat accgccggcg ccgccggcga acctggccgg gaccatcatc ctgttctgcg
     4681 accaggggct cgccgctccg gtcggcagca cggtgacggt gaacgacgcg gcgatcccgg
     4741 ccgtcggcca ggcgttcctg tactgggccg gctacagcag cccgacggcc ggcgccaaaa
     4801 ccgccctcgg tcggaggacc gacaccacga tccggatcgc gcccttgact tgtccgtagg
     4861 agggagcgtt cgcggcgcgg gggggacgtc cggaccccgc gccgcggacg gcgcgaaggg
     4921 agagactcgg acaggtccct actggaagtc gcgggggatc ggctcgtccc aggtcttctc
     4981 gcgcacgagc accccgtcct cgaagagccg ccgcgtatac gtcaggtaga agttcttcgc
     5041 gtcgctccgg aagaccgccc gcgcctcgaa gcgcagcgtc cggtcgggga gcgtgacggt
     5101 cgtgctgtag tccccggtca tcgaggcggc ctcggggtgc gcgtcgcccg cctcgtggcg
     5161 aatcgactcg gtgttgtgct ccgtccccca ggggaaccgg ctcccgccgc cgttcgtcgc
     5221 cgtgatgcgc gcctcacccg tggggggatg ccgctcgatc gactcgatct cgccgtagcc
     5281 ggaggcggtg ccggtctcga gcacgccgaa ttcagccagc tcttcgttct tctccggcgg
     5341 caggaagttc gggcgcggcc tttcggcctt cgggacgacc ggaaggcgca ggcgcgtgcc
     5401 gtcggcgccg cccagcagca gcgtcgtcgt catcgggtgc ggcgtcggcc agatcatcgg
     5461 ccacatggca ttcgacaccg cgaaacgcat gcgatgtccc ggcgcgaaga cccaggacgt
     5521 gaagtgcatc tcgatgtcca gcgtgaacgg cttccccggc tcgatcgctc tcggctcgcg
     5581 cgccgagacg cggtgcgcgc cgttctcgcc ggcgccggcg acgagcgtca cggtgccgtc
     5641 gggggcgacg tccgacagcc gcgcgaacca gcgggccagg ggcgcgtcgg cgctgacccg
     5701 caggacggcg cgcggcaggc cgaggatctc cacctcctcg gtgagcggct cggtgtcgta
     5761 caccaggctg taggcgtcgg acggacgctg gtcgggggcg acgtcccccc accacatgac
     5821 cggcccgccg atctcgacgc cggaggtcgg cacgtagcgc agcctgtgcg ccgccgcggg
     5881 tgggctcacg gagccggccg caagccgcgc cgtctccatc gccggcccga gggtgtgatc
     5941 gtcgcgtggc gagagcgcca gctcccggcc gcgggcgaga ggccagccat cctcgtaacg
     6001 ccattcgccc ggggcgtttc cttcgaggcg cgggccgggc ggatgccagc ggcgcacgaa
     6061 gacggcgagg cggggctcac ggtcgatgcc ggtctccttc cccttgagcc agcggtcgaa
     6121 ccagcggacc gcctcgtgcc gccattccat ccccggcttc gggtaggcat cgtgcgggaa
     6181 gcagtgcgac cacgggccga cgatcgcttt catgggcgcc ttcacgtgct cgagcatgcg
     6241 cggcacgctg tcgcgatacc cgtcgtacca gccgccgatg acgaaggtcg ggatgcggat
     6301 cgactcgtag cgggccttga gcgcggtgcg gtcccagaac ggcccgtccc gctgctgctt
     6361 cttgtaggtg aacatccagg gccgctggtc gaagcgatcg gcgaacagcc ggtcgtcgat
     6421 gacgtagccg ggggcgcccg gcatggcgtt cgccaggccc atgctcatct cccaggaatc
     6481 gacatggatg ccgccgtcca tgaagtgcac gtcgtcctgg tagaggtcgt cggtggcgtc
     6541 cacg
//
LOCUS       DASRIQ010000161        16115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1122730, whole genome shotgun sequence.
ACCESSION   DASRIQ010000161 DASRIQ010000000
VERSION     DASRIQ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 16115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16115
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122730"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..393
                     /gene="rpmB"
                     /locus_tag="VFD06_03865"
     CDS             199..393
                     /gene="rpmB"
                     /locus_tag="VFD06_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZN02706.1"
                     /translation="MARSCDVCGKGPVFGNQISHAHNVSPRVWYPNIQKVRVVVGKSV
                     RRKRVCTRCLRSGAVVKAAS"
     gene            complement(480..866)
                     /locus_tag="VFD06_03870"
     CDS             complement(480..866)
                     /locus_tag="VFD06_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN02707.1"
                     /translation="MTNQAEIIRTDRAPQAIGPYSQAIRAGDLVFLSGQVGLDPATGA
                     LVEGGLEAETTRALQNLEAVLRAAGAGLDRVVRTTVFLVDLGDFATMNAIYARAFGET
                     RPARSTVQVSALPRGARVEIDAIAVL"
     gene            complement(877..3024)
                     /locus_tag="VFD06_03875"
     CDS             complement(877..3024)
                     /locus_tag="VFD06_03875"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZN02708.1"
                     /translation="MIRFEDVQSKVQSYLKDADLSLLKGAYEFSAKEHKGQTRSSGEP
                     YLVHPLSVAAILADLKLDLTCIVAGLLHDVLEDTLTTREILEERFGRDIAHVVEGLTK
                     ISRIRFNSREQQQAESFRKMMLAMVDDIRVVLVKLADRLHNMRTLEHLSARQQERIAR
                     ETMEIYAPLAHRLGIGRIKNELEDLALKYLDPAGEEQLQRALEEKSRLTVDFIREIES
                     TLRTELGRAGIECSITGRRKHLYSIYKKIKRQRIDVCEVYDYIAFRVLTGTIKDCYGA
                     LGIIHGLWRPVPGRIKDFIAMPKPNMYQSLHTSVISDRGQPFEVQLRTHDMHRIAEEG
                     IAAHWKYKEGSGGKEPDANIQWLRQIMEWQQELKDPAEFLRMVKVDLYPDEVYCFTPQ
                     GQVKSLPRGGTPVDFAYSVHTEVGHHCAGARVNGKLQPLRTELKNGDIVEILTSPNHS
                     PSPDWLAFVQTPRARTKIRQWLNSERRSRAVELGKTLTDREFKRFRFNLKPHVQEGTR
                     LREALLGMGFPSIEDFYAGVGYGKAVPKALVEKLDPQAKPQDVPEGGITQVVKRALGL
                     SGKKVAVRGLDDALIILARCCNPIRGEPIVGYITRGRGVTVHSERCPNLEKLLYDPER
                     RIEVVWEGADESRFDVRLTVFSEDRPGILARITQAIADAKSNIKHVEARTFEDRRGEI
                     TLVLDIRDVDHLQKIVERLKGIDGVYHVERQVA"
     gene            complement(3199..4374)
                     /gene="secF"
                     /locus_tag="VFD06_03880"
     CDS             complement(3199..4374)
                     /gene="secF"
                     /locus_tag="VFD06_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZN02709.1"
                     /translation="MLQVFHNAGFQLMGRTKYYFMAFSIVCMTLAIVFIAGGFNYGID
                     FAGGTAVQVRFAERPSLDHLRSALDAAGLGDVTLQQIGDPKDHEILIRVERDAKAEAG
                     SGEGGGVSQRVIRALRELDGPPATDRIDLNAASESTLREWIASRLPAGEAGATPPDAA
                     AAAAAIVRARTEHGGLFSDPAQVAAIPGIPEALRPVLRDGSTLGKFAVRGVDFVGPTA
                     GAELMQNTLWAIVGAVTGILMYVWLRFHKIAWGVAAVIALVHDVVIAAGAVALTNKEF
                     SLTVVAALLTILGYSINDTIVVFDRVRENLRLYRDHDFERVVNASVNQTLSRTMLTVF
                     TVFVAVLALFLYGGEKLNPMSFCLLVGIVFGSYSSVFVAAGLLVIAYRWLGAKHVRV"
     gene            complement(4385..5983)
                     /gene="secD"
                     /locus_tag="VFD06_03885"
     CDS             complement(4385..5983)
                     /gene="secD"
                     /locus_tag="VFD06_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZN02710.1"
                     /translation="MNLSDVRVRLAIVLGSVILCAVLIYPLSEKISLGLDLRGGIHMV
                     MRVRTDEAVKADIDLARERIRAALKEKGITVGAMDSPELEALTLAGIEPARLDETRTL
                     LRDQFPQYEVQSGSGALQLRLRAADEAATRDSAVRQALETIRTRIDKFGVAEPIIQRK
                     GVGPQADQIVVQLPGVDDPERVKDLIGSPAFLEWKMVKVPPGITADQFRAAPSPEALA
                     AQFGAALPPDIEALPQEMRDQEGRLQTVWWPVTRSSPLTGADLKNARRDQDRFGGAAV
                     NFLLTAEAGQRFQELTRANQGQLLAIVLDRKILSAPRINAVIAERGIIEGGNFTIESA
                     EDLSLKLRSGALPAGMDILEERTVGASLGADSIKQGVVASVIGSIVVILFMLIYYRLS
                     GVNATVVLVINILFLLAAMASLRSTLTLPGIAGIALTVGMAVDANVLVFERIREELRG
                     GRTIKAAIQAGFQKALSTIVDSNLTTLIAAFALLAFGTGPVKGFAVTLSVGILANLIT
                     AVFMSKTLFDFLLTLRPRIAQLSI"
     gene            complement(5991..6320)
                     /gene="yajC"
                     /locus_tag="VFD06_03890"
     CDS             complement(5991..6320)
                     /gene="yajC"
                     /locus_tag="VFD06_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZN02711.1"
                     /translation="MGPLGMMAPPQGTGQPNPWLQLVPLLFFFVVIYFLLIRPARTRQ
                     KQTQAMLEALRPGDKVTTSGGLLGTVVAVDREVVQLRIADKVKVDVTRNAIVHRQEGS
                     GGAVPEA"
     gene            6548..7297
                     /gene="pgeF"
                     /locus_tag="VFD06_03895"
     CDS             6548..7297
                     /gene="pgeF"
                     /locus_tag="VFD06_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZN02712.1"
                     /translation="MLPYHLSGHGTARRITLPLFERLPGVAHGFTVKGSIPPDAVAGA
                     VGRPMPLLTLRQVHGAEVRLLGDEESLWGEGADRPQGDALLTRRIDAAIGIAVADCVP
                     ILLADPVSGWAGAVHAGWRGTAAGVLRAAIAALTARGVPVDALYTGIGPSIGPCCFEV
                     GEEVVEALLRSDPEAGASVRRGAAKPRVDLVELNRRQALAAGVRSDRIAAAGLCTVCH
                     EDMLESYRRSRGAQGRMIGFVTRTAPPPLTS"
     gene            complement(7287..8642)
                     /locus_tag="VFD06_03900"
     CDS             complement(7287..8642)
                     /locus_tag="VFD06_03900"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02713.1"
                     /translation="MTPSRFARLVAPGALVLLGLLAAPPAFAEGMTKVRGTVTDPQGK
                     PLEKVPIFFEATDIKKIVGPVKTNKKGEYLIATLDKSVAQKWKVYPKLDGYKAVIMYW
                     TIVGSDGSEQDKSEKLFDSKQEFPDFPLVLVGDAGRNQFDFVIAKDAEFAAANQAIQK
                     KRKEAQAGAAGAATAQAGAPAAAVPAAPEPPKINAEAAQSLQKAKSLADAGNHPQAIE
                     LYKAFLAKDPTSNPAVYYYLGKSLFETGDDAAAAQSFSKGLELKQDMKGAHFYLGNLS
                     LRQEDAAGAVAEYEKELTLSPDSDTVLYNLGQASIKVGDYDKAIAALDKAVAINPQKP
                     ESLMLLASAYEHKGDKVKADELYQKVAAIDPRNAAILFYNVGVKAWNEKRGKDAAQAY
                     RKAVEIDPNYAQAHRELAVALMSQQDFKGALVHFQEYLKLEPKAPDMKEIQDNIALLK
                     K"
     gene            complement(8738..9211)
                     /locus_tag="VFD06_03905"
     CDS             complement(8738..9211)
                     /locus_tag="VFD06_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02714.1"
                     /translation="MSRFERLLLDLSIGLSTLTGLVYFAMKEWMRPRDPFSILGHPWQ
                     PHVLALHVLAGPVVIFALGLIAREHVFERIVAGRPLTGRKTGLFIAGLALPMVGSGYA
                     LQIVTAPAGRRALALLHLASGLLFALLFVGHLLATAARRRTASGRRAIVPDPFSS"
     gene            complement(9208..9780)
                     /locus_tag="VFD06_03910"
     CDS             complement(9208..9780)
                     /locus_tag="VFD06_03910"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZN02715.1"
                     /translation="MALLGSVRTAACAAILALGIAAPAGARVLMTQEEALRRAFPPPQ
                     TWERKTLYLDEAQAARAAREAGAPVESRVVPYYVGAHGGRITGYAYFDTHLVRTLPET
                     ILVRLRPDGAIEAIDVIAFDEPDEYRTTPRWLQQFQGQGGAGSADLGGRIRSLTGATL
                     SARAVTEAVKRIVALHRLFVAPPAGAGRVK"
     gene            complement(9768..11144)
                     /locus_tag="VFD06_03915"
     CDS             complement(9768..11144)
                     /locus_tag="VFD06_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02716.1"
                     /translation="MTIALLAAASGAAVAAEPAAGQAAAAGESAATDDLARRLRDLEE
                     QVSALKAEMARGRGAAVPAADAADLEKRIEALSKEIERLRLGEAAAPEADKSVGGFGP
                     AASKVYQTKRGVAIGGYGEFIYTDPDDSQDDGAASGETASADLLRAVLYFGYKFNDKL
                     LFNSEIEVEHALAADGADGEVAVEFAYVDYRHNDAFGGRGGLLLVPVGFINELHEPPI
                     FFGSTRPEVERFIIPTTWRELGGGVYGSAGPVDWRAYLVTSLDASGFSADTGIRGGRQ
                     EGSEAVAEDFGLTARVDWTVRPGLVLGVGGFTGDTAQANVGVDGARVTLYEGHAEWLW
                     RGLKVRALYARTSLDDAAQVGTLTGEAVGSQMNGYYGEVGWNVLSLRKSSRQELSPFF
                     RYESLNTQAEMPTGVLADPANDRTVHTVGIHYRPIPNIALKADWQGFSNAAGTGVDRI
                     NLALGWLF"
     gene            complement(11219..12295)
                     /locus_tag="VFD06_03920"
     CDS             complement(11219..12295)
                     /locus_tag="VFD06_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZN02717.1"
                     /translation="MRTRWRWTAVVAACLVVPAAGPGPGADPATAPVERARFLMGTRL
                     VIEASGPRAEESIDRAFGEVERLDQVLSNWRDDSEIALLNRAAAEGAVTCSADLFGAV
                     AGSLTWAEKTDGAFDPTVEPLVRRLGLRGDEGRLRGAAPEAPQGPGPDPIGWRLVEIR
                     PDAKSVRFLAPGVGIDFGGIGKGIALDAAAGILKGAGVSAARLDFGGQVLVFGRGPDD
                     GGWIIGLSDPRDRDHLVGTFVLRAGSIATSGNSERAAGGPDGPRSHLLDPRLGAAARF
                     DGSLTVVAPDATSADALSTALFVMGPDRGLAYAADHRLDVLYLRRDRDGALVRRGRGA
                     LAGSAPGDSFPTTQSPGQPGEPHS"
     gene            12459..12962
                     /locus_tag="VFD06_03925"
     CDS             12459..12962
                     /locus_tag="VFD06_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZN02718.1"
                     /translation="MAEQPRPVSASRVAMTEIVMPEDTNPYGNVYGGRVMALIDKAAA
                     IAAMRHCRANVVTALVDSLEFRGPVRLGHILRLHAEVHAVFGTSLDVGVEVTSEDPLT
                     GRVAHCCSASVLMVSLDARGRPAQVPRLKPRGAVERERERAARSRRRARLRRRGPRPP
                     ASAASAV"
     gene            13034..14242
                     /locus_tag="VFD06_03930"
     CDS             13034..14242
                     /locus_tag="VFD06_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZN02719.1"
                     /translation="MSAPKVLGAAPLPHPGGGDERLLELMQIARSITSSLRLDEVLGR
                     VIDGAVRFSGAQRGFLFLKSADGRLVRWPAPPDGAPPVEVSLSVAEEVARSGRPVHRD
                     HLAGSDTASLTDSIVRLRLMAILCVPLLAQSEVIGVIYLDSRRPMPHQAHDLDLLEAL
                     AGLAAIAIQNSRLVEERLRAERTLALGRTARALVHDLRSPLTAIRALAELLHGRAAGD
                     AATRRHLASIMDEADRLAELASDLLRFAGGAPPLRREAVVLGPYLTRLLEPLRPRLES
                     SGVALVTDLEPTAVAPLDAPRAARVVHNLVANALDAMPDGGTLTVACGTTGGRAWLRI
                     GDSGCGMSEAVRSRLFEPFFTHGKEHGTGLGLALARAIVEEHGGRIEVESAPGRGSVF
                     TIELEAAPSA"
     gene            complement(14239..15738)
                     /locus_tag="VFD06_03935"
     CDS             complement(14239..15738)
                     /locus_tag="VFD06_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZN02720.1"
                     /translation="MTSRLVIDSLPGERRVALIEDDLLVELHVERAGARPVAGNVYLG
                     RVRNVLPGMQAAFVDLGLERDGFLHVDDAPGPARLRPEWAGDDTDAAPPGPAAPAPRI
                     EERVREGEEIVLQVAKDPLARKGPRVTAHLSMPGRLVVLLPTVEHLGVSRRIASEEER
                     LRLRELLRDLHERTRPPGGLILRTAGAEADPAALQEEILLLAETWRGIQARRAEARAP
                     ALLSEEAGVIERVLREQLRHGVEEVAVDSDDALEEVRRALARLGAAGPRIVRHEGPEP
                     LFDARRLTTQIDRALKARVWLPSGGHIAIHPTEALVAVDVNTGKYVGRGDLEETLLRT
                     NLEAVKEIARQIRLRDLGGLIVIDFIDMQRPESREAVRTALAERLQRDRARSRMLPMS
                     EFGLVEITRQRSRPSLDRQLLRACPACDGAGRVRSLPTLQQEIVRQARRLGARPEGTV
                     VARLAPEAAAGLRPLIPEMARALGGIDPGRLALEGDPQMGPDDVLVVER"
     gene            complement(15741..>16115)
                     /locus_tag="VFD06_03940"
     CDS             complement(15741..>16115)
                     /locus_tag="VFD06_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZN02721.1"
                     /translation="KGFLSGTQGSLQFLPEQHTDFVLAVLAEERGFLGSALVLALYFI
                     VLTRCVSTARVARDRLGAFLAMGVACVFGGQALINIGVVLGVLPTTGVPLPLLSYGGS
                     CLVSTLLGLGLVLNVHMRRLVN"
BASE COUNT         2300 a         5791 c         5618 g         2406 t
ORIGIN      
        1 tctatcagaa gtgaacagcc gctatttcgg aatcgaatat gtatacttgc ggaagaaacg
       61 gcaagccctt gatagcccgg gccttgggtt gcctgccggg ggagggggtc ggagcttgag
      121 ccgactgtcg ggattgtgct agagtttccg acttccgtaa cccagcaacc tgggcttcaa
      181 gcccctcgga ggcagtcggt ggcgcgatcc tgtgatgtct gcggcaaggg gccggtcttc
      241 ggaaaccaga tcagccacgc gcacaatgtg tcgccgcgcg tctggtaccc caacatccag
      301 aaggtccggg tcgtggtggg aaaatcggtg cgccgcaagc gcgtctgcac ccgctgcctg
      361 cgctctggcg ccgtcgtgaa ggcagcttcc tagctccaac cgaaatcgat ctcagcgctt
      421 caggggaaac gggaacgccc ccgttgcccc taacaccgag agattgggga gggctcgact
      481 caaagcacgg cgatcgcgtc gatctcgacg cgggcgccgc gcggcagcgc cgacacctgc
      541 accgtcgagc gggccggacg cgtctctccg aaggcgcgcg cgtagatcgc gttcatcgtc
      601 gcgaagtcgc cgaggtccac gaggaagacg gtcgtccgca ccacccgatc gagtcccgcc
      661 cccgccgccc gcagcacggc ctcgaggttc tgcagcgccc gggtcgtctc cgcctccagt
      721 cccccctcga ccagcgcccc ggtcgcgggg tcgagtccga cctgccccga gaggaacacc
      781 agatcccccg cccggatcgc ctggctgtag ggaccgatcg cctggggcgc ccggtcggtc
      841 cggatgatct ccgcctgatt ggtcacggaa ccctcctcac gccacctggc gttcgacgtg
      901 gtacacgccg tcgatcccct tcaaccgctc gacgatcttc tggaggtgat cgacgtcccg
      961 gatgtcgagg acgagcgtga tctcgccgcg ccggtcctcg aaggtgcgcg cctcgacgtg
     1021 cttgatgttc gacttggcgt cggcgatcgc ctgggtgatg cgcgccagga tccccgggcg
     1081 gtcctccgag aagaccgtca ggcggacgtc gaagcgcgac tcgtccgccc cctcccagac
     1141 cacctcgatg cggcgctccg ggtcgtagag gagcttctcg aggttcgggc agcgctccga
     1201 gtgcaccgtg acgccgcggc cgcgggtgat gtagccgacg atcggctcgc cgcggatcgg
     1261 gttgcagcag cgggccagga tgatgagggc gtcgtcgagg ccgcggaccg ccaccttctt
     1321 ccccgagagc cccagcgccc gcttgacgac ctgggtgatc cccccctcgg ggacgtcttg
     1381 gggcttcgcc tgcgggtcga gtttttccac cagcgctttc gggaccgcct tgccgtagcc
     1441 gaccccggca tagaagtcct cgatcgacgg gaaccccatc ccgagcagcg cctcgcgcag
     1501 gcgcgtcccc tcctgcacgt gcggcttgag gttgaagcgg aaccgcttga actcccggtc
     1561 ggtcagcgtc ttgcccagct cgacggcgcg gctgcgccgc tcggagttga gccactggcg
     1621 gattttcgtg cgggcgcgcg gcgtctggac gaacgccagc cagtcggggc tcgggctgtg
     1681 attgggggag gtcaggatct cgacgatgtc gccattcttc agctcggtcc tgagcggctg
     1741 cagcttgccg ttgacccggg cgccggcgca gtggtgcccg acctccgtgt gcaccgagta
     1801 ggcgaagtcg acgggggtcc cgccgcgcgg cagcgacttc acctgcccct gcggagtgaa
     1861 gcagtagacc tcgtccgggt acaggtcgac cttcaccatt cgcaggaact cggccgggtc
     1921 cttcagctcc tgctgccact ccatgatctg gcgcagccac tggatgttgg cgtccggttc
     1981 cttcccgccg ctcccctcct tgtacttcca gtgcgccgcg atcccctcct cggcgatgcg
     2041 gtgcatgtcg tgcgtccgga gctgcacctc gaacggctgg ccgcggtcgc tgatgaccga
     2101 cgtgtgcagc gactggtaca tgttcggctt cggcatcgcg atgaagtcct tgatgcggcc
     2161 cgggaccgga cgccagagcc cgtggatgat cccgagcgcg ccgtagcagt ccttgatggt
     2221 gccggtgagg acccggaacg cgatgtagtc gtacacctcg cagacgtcga tgcgctgccg
     2281 cttgatcttc ttgtagatgc tgtagaggtg cttgcgccgc ccggtgatgc tgcactcgat
     2341 gccggcacgc ccgagctcgg tcctcagcgt cgactcgatc tcgcggatga agtcgacggt
     2401 caggcggctc ttctcctcga gggcgcgctg cagctgctct tcgccggccg ggtcgaggta
     2461 cttgagcgcc aggtcctcca gctcgttctt gatgcgcccg atgccgaggc gatgcgccag
     2521 cggcgcgtag atctccatgg tctcgcgcgc gatgcgctcc tgctggcggg ccgacaggtg
     2581 ctcgagggtg cgcatgttgt gcagccggtc ggcgagcttg acgagcacca cgcggatgtc
     2641 gtccaccatc gccagcatca tcttgcggaa cgactcggcc tgctgctgct cgcgcgagtt
     2701 gaaacggatg cgcgagatct tcgtcaaccc ctcgacgacg tgcgcgatgt cccggccgaa
     2761 gcgctcctcg aggatctcgc gcgtcgtcag ggtgtcctcg aggacgtcgt gcagcagccc
     2821 cgcgacgatg caggtcaggt cgagcttgag gtcggccagg atcgccgcca ccgacagcgg
     2881 gtgcaccagg tacggctcgc ccgaggagcg ggtctggccc ttgtgctcct tcgcggagaa
     2941 ttcgtaggcg cccttgagca atgagagatc ggcgtccttg aggtacgact ggaccttcga
     3001 ctggacgtct tcgaagcgga tcatggaacg aggcgcatcc tggtcacggc gcgtcggggc
     3061 ggggccgggg ccgcggaccg ccgcgcgaaa cgcgcgacgc ccgggacgct cgaaagttta
     3121 gcacccgcgc ggcggaccgc gcaacgcgcg cttggtgcgg cggaacgccg gtccggctcc
     3181 gaacgggcgc ctcgcgggct agacgcggac gtgcttggcg ccgagccagc ggtaggcgat
     3241 caccaggagc ccggcggcga cgaacaccga ggagtaggag ccgaagacga tcccgaccag
     3301 caggcagaac gacatcggat tgagcttctc gccgccgtac aggaacaggg ccaggaccgc
     3361 gacgaacacc gtgaagaccg tcagcatcgt ccggctcagg gtctggttga ccgaggcgtt
     3421 caccacgcgc tcgaaatcat gatcgcggta gaggcgcagg ttctctcgga cccgatcgaa
     3481 caccacgatg gtgtcgttga tcgaataacc caggatggtc aagagcgccg ccacgacggt
     3541 cagggagaac tccttgttcg tgagcgcgac ggccccggcg gcgatgacga cgtcgtggac
     3601 cagcgcgatg accgccgcca ccccccaggc gatcttgtgg aagcggagcc agacgtacat
     3661 caggatcccg gtcaccgcac cgacgatggc ccacagcgtg ttctgcatca gctccgcgcc
     3721 cgccgtcggc ccgacgaaat cgacgccgcg cacggcgaac ttgcccaggg tcgatccgtc
     3781 gcgcaggacc ggacggagcg cttcgggaat ccccgggatc gcggccacct gggccggatc
     3841 gctgaacagg cccccgtgtt ccgtgcgcgc ccgcacgatc gccgcggcgg ccgcggcggc
     3901 gtcgggagga gtggcccccg cctcgcccgc cggcaggcgg gaggcgatcc actcgcgcag
     3961 cgtggactcg gaggccgcgt tgagatcgat gcgatccgtg gccggcgggc cgtccagttc
     4021 ccgcagggcc cggatcaccc gctggctgac gcctcccccc tcccccgatc ccgcctcggc
     4081 ctttgcgtcc cgttccacgc ggatgaggat ctcgtgatcc ttgggatcgc cgatctgctg
     4141 cagggtgacg tccccgagcc cggcggcgtc cagggcggag cgcagatgat cgagggacgg
     4201 ccgctctgcg aagcggacct gcaccgcagt cccgccggcg aaatcgatgc cgtaattgaa
     4261 gccccccgcg atgaagacga tcgccagcgt catgcagacg atggagaagg ccatgaagta
     4321 gtacttcgtc cgccccatca gttggaaccc ggcgttgtgg aacacctgca gcatcggcgc
     4381 ggcctcagat gctcagctgc gcgatgcgcg gccggagcgt gagcaggaag tcgaacagcg
     4441 tcttcgacat gaacacggcg gtgatcagat tggccaggat gccgacgctg agcgtcaccg
     4501 cgaatccctt gaccggcccc gtcccgaacg caagcagggc gaaggccgcg atcagggtcg
     4561 tcaggttgct gtcgacgatg gtcgacaggg ccttctgaaa tccggcctgg atcgccgcct
     4621 tgatggtgcg accgccgcgc agctcctcgc ggatgcgttc gaacaccagc acgttggcat
     4681 cgaccgccat gccgacggtc agcgcgatgc cggcgatgcc gggaagcgtc agggtcgagc
     4741 gcaggctggc catcgcggcg agcaggaaca ggatgttgat caccaggacg acggtggcgt
     4801 tcacccccga cagcctgtag tagatcagca tgaacaggat gacaacgatc gagccgatga
     4861 ccgacgcgac gaccccctgc ttgatcgagt ccgcaccgag cgaggcgccg acggtgcgct
     4921 cctccaggat gtccatcccg gcaggcaggg cgccgctccg gagcttgagg gacaggtcct
     4981 cggcgctctc gatggtgaag ttgcccccct cgatgatgcc gcgctcggcg atgaccgcgt
     5041 tgatgcgcgg cgccgacagg atcttccggt ccaggacgat cgccaggagc tgcccctggt
     5101 tggcgcgcgt cagctcctgg aagcgctgcc ccgcctcggc cgtgagcagg aagttgaccg
     5161 ccgcaccgcc gaagcggtcc tgatcccgcc gcgcattctt cagatcggcg ccggtcagcg
     5221 gcgacgagcg ggtcaccggc caccacaccg tctgcaggcg tccctcctga tcgcgcatct
     5281 cctgcgggag cgcctcgatg tcgggcggca gcgccgcgcc gaactgcgcc gcgagcgctt
     5341 cgggactggg ggccgcccgg aactgatccg ccgtgatgcc cggcggcacc ttcaccatct
     5401 tccactccag gaacgccggg ctgccgatca ggtccttgac ccgctcgggg tcgtccaccc
     5461 ccggcaactg cacgacgatc tggtccgcct gcggcccgac ccccttgcgc tggatgatcg
     5521 gctcggcgac gccgaacttg tcgatgcggg tccggatggt ctcgagcgcc tgccgtacgg
     5581 cgctgtcccg cgtcgccgcc tcgtcggccg cccgcagccg gagctgaagg gcgccgctgc
     5641 ccgactgcac ctcgtactgc gggaactggt cgcgcaggag cgtgcgcgtc tcgtcgagcc
     5701 gcgccggctc gatcccggcg agcgtcaggg cctcgagctc agggctgtcc atcgccccga
     5761 cggtaatccc cttctccttc aacgccgcgc ggatccgctc gcgggccagg tcgatatccg
     5821 ccttgaccgc ctcgtcggtg cgcacccgca tcaccatgtg gatgccgccg cgcaggtcga
     5881 ggccgaggct gatcttctcc gacagcggat agatcagcac cgcgcacagg atgacggacc
     5941 ccaggacgat cgcgagacgt acccgtacgt cggacaggtt catgctcccc tcacgcctcc
     6001 ggtaccgcgc cgccggagcc ttcctgccgg tggacgatgg cgttgcgcgt cacgtccacc
     6061 ttgaccttgt cggcaatccg cagctgcacg acctcgcgat cgacggcgac gaccgttccg
     6121 agaagaccgc ccgaggtggt caccttgtcg ccgggccgca gcgcctcgag catcgcctgg
     6181 gtctgcttct gccgcgtccg cgccggccgg atcagcagga aatagatgac cacgaagaag
     6241 aagaggagcg ggaccagctg cagccacgga ttcggctgcc cggtcccctg cgggggggcc
     6301 atcatgccca acggacccac gctctcactc ccgggctccg gcatccctcg cgcgtgtgcg
     6361 ctgcggcgcc gggccaaaca aaaaggaggc gtgtcaggcc tccgtccaag ggccggcggc
     6421 ggtccgccgg tcgcatgcca tggtgcgagg ggcggtagat tgccgcaagc ccttctggtt
     6481 gtcaaggcgc cgtgcgacgt cccgcgcgcg cttgcgggag gtgaagccgg ctcgtacact
     6541 cccggggatg ctcccctacc acctctcggg gcacgggacc gcgcgtcgca tcacgctgcc
     6601 gctgttcgag cgcctgccgg gggtcgcgca cggcttcacc gtgaagggat cgatccctcc
     6661 ggatgcggtg gccggagcgg tcgggcggcc gatgccgctc ctcacgctcc gccaggtcca
     6721 cggagcggag gtgcgtctcc tgggcgacga ggagtcgctc tggggggagg gcgcggatcg
     6781 cccgcagggg gatgcgctcc tgacgcgaag aatcgacgct gcgatcggga tcgccgtggc
     6841 cgactgcgtc ccgatcctgc tcgccgaccc ggtgtccggc tgggccgggg cggtgcacgc
     6901 cggctggcgc gggacggcgg ccggcgtcct gcgcgcggca atcgcggccc tcaccgcgcg
     6961 tggcgtgccg gtggacgcgc tctacacggg gatcgggccg tcgatcggac cctgctgctt
     7021 cgaggtcggc gaggaggttg tggaggccct gctgcgaagc gatccggagg cgggggcgtc
     7081 ggtgcgacgg ggcgcggcga aaccgcgcgt cgacctcgtc gaactgaacc gccgtcaggc
     7141 gctcgcggcc ggtgtgcgca gcgaccgcat cgccgcggcc ggcctgtgca ccgtctgcca
     7201 tgaggacatg ctggagtcct accgccgcag ccgcggcgcg cagggacgga tgatcggctt
     7261 cgtcacccgc accgcaccgc cccccctcac ttcttgagga gggcgatgtt gtcctggatc
     7321 tccttcatgt cgggcgcctt cggctcgagc ttcaggtact cctggaagtg gaccagcgcc
     7381 cccttgaagt cctgctgcga catcagggcc accgccagtt cgcggtgcgc ctgggcgtag
     7441 ttcgggtcga tctccaccgc cttccggtag gcctgcgccg cgtccttgcc gcgcttctcg
     7501 ttccaggcct tcacgcccac gttgtaaaag aggatggcgg cgttgcgcgg atcgatggcc
     7561 gcgaccttct ggtagagctc gtccgccttc accttgtccc ctttgtgctc gtaggcggag
     7621 gcgaggagca tcagcgactc cggcttctgc ggattgatcg ccaccgcctt gtccagcgcc
     7681 gcgatggcct tgtcgtagtc accgaccttg atggaggcct ggccgaggtt gtagagcacg
     7741 gtgtcggagt cgggcgagag ggtcagctcc ttctcgtact cggccaccgc ccccgctgcg
     7801 tcctcctggc gcagcgacag gttgccgagg tagaagtgcg cccccttcat gtcctgcttc
     7861 agctccagcc ctttggagaa ggactgtgcc gcggcggcgt cgtcgccggt ctcgaacagg
     7921 ctcttcccga ggtagtagta gacggccggg ttcgaggtcg ggtccttggc gaggaacgcc
     7981 ttgtacaact cgatcgcctg cgggtggttg ccggcgtccg cgagcgactt cgccttctgc
     8041 agcgactggg ccgcctcggc gttgatcttc ggcggctccg gggcggcggg caccgcggcc
     8101 gccggggccc ccgcctgggc ggtggctgcg ccggcggcgc ccgcctgcgc ctccttgcgc
     8161 ttcttctgga tcgcctggtt ggcggcggcg aactcggcgt ccttggcgat cacgaagtcg
     8221 aactggttgc gcccggcgtc ccccaccagg accaggggga agtccgggaa ctcctgcttg
     8281 gagtcgaaga gcttctcgct cttgtcttgt tccgagccgt ccgagccgac gatggtccag
     8341 tacatgatga ccgctttgta gccgtcgagc ttcgggtaca ccttccactt ctgcgccacg
     8401 gacttgtcga gcgtcgcaat gaggtactcg cccttcttgt tggtcttcac ggggccgacg
     8461 atcttcttga tgtcggtggc ctcgaagaag atcggcacct tctcgagcgg cttcccctgc
     8521 ggatcggtga ccgtgccgcg caccttggtc attccctccg cgaaggcggg gggggcggcc
     8581 agaagcccca ggaggaccag ggcccccggg gcgacgaggc gggcgaagcg agaaggcgtc
     8641 atgacgacct ccggacgcgc ccggatcggg cgcgacagtc gattgggacg ggcgcaggaa
     8701 ttctagaaca cgctcccctc cggatcaaca cgggggccta cgacgagaag ggatcgggga
     8761 cgatcgcccg gcgccccgac gccgtccgac gccgcgccgc cgtcgccagc aggtgaccca
     8821 cgaacaggag ggcgaagagg agccccgagg ccaggtgcag gagggccaga gcccgccgtc
     8881 ccgccggggc ggtgacgatc tggagcgcat agccgctccc caccatcggc agcgccaggc
     8941 cggcgatgaa caggccggtt ttcctcccgg tcagcggccg gccggcgacg atccgctcga
     9001 agacatgctc gcgcgcgatg aggccgaggg cgaagatcac caccggcccg gccaggacgt
     9061 gcagcgccag gacgtgcggc tgccaggggt gcccgaggat ggagaaggga tcgcgcggcc
     9121 gcatccactc cttcatggcg aagtacacga gtccggtgag cgtggagagc ccgatcgaga
     9181 gatcgaggag cagccgctcg aagcggctca cttcaccctc ccggcaccgg ccggcggcgc
     9241 gacgaacagc cggtgcaggg cgacgatgcg cttcactgcc tcggtgaccg cccgcgccga
     9301 cagggtcgcg ccggtgagag agcggatccg cccgccgagg tcggcgctcc cggctccccc
     9361 ctgtccctgg aactgctgca gccagcgggg ggtcgtgcgg tactcgtccg gctcgtcgaa
     9421 ggcgatgacg tcgatcgcct cgatcgcccc gtccgggcgg agccggacga ggatcgtctc
     9481 cggcagcgtg cgcaccaggt gcgtgtcgaa gtacgcgtag ccggtgatcc gcccgccgtg
     9541 cgcgccgacg tagtacggca cgacgcgcga ctccaccggc gcccccgcct cgcgcgccgc
     9601 gcgcgcggcc tgcgcctcgt cgaggtagag ggtcttgcgc tcccaggtct gcggcggcgg
     9661 gaaggcgcgg cgcagcgcct cctcctgcgt catgaggacg cgggcgccgg ccggcgccgc
     9721 gatccccagc gcgaggatcg cggcacaggc cgccgtgcga acgcttccta gaagagccac
     9781 ccgagcgcca ggttgatgcg gtcgacgccg gtcccggcgg cgttggagaa gccctgccag
     9841 tccgccttca gcgcaatgtt cgggatcgga cggtagtgga tgccgacggt gtggaccgtc
     9901 cggtcgttgg ccggatcggc gaggacgccc gtcggcattt ccgcctgcgt gttgagcgat
     9961 tcatatcgga agaacggaga cagctcctgc ctcgagcttt tccggagcga cagcacgttc
    10021 cagcccacct caccatagta gccgttcatc tgcgatccga cggcctcgcc ggtcagcgtg
    10081 ccgacctggg ccgcgtcgtc gagactcgtt cgcgcgtaga gggcgcggac cttgagaccg
    10141 cgccacagcc actcggcgtg cccttcgtac agcgtgaccc gcgccccgtc gaccccgacg
    10201 ttggcctggg cggtgtcccc ggtgaatccg cccacgccga ggacgagccc cggccgcacc
    10261 gtccagtcga cccgcgccgt caggccgaag tcctcggcga ccgcctccga tccctcctgc
    10321 cgcccgccgc gaatccctgt gtcggcggag aagcccgagg cgtcgaggct cgtgacgaga
    10381 taggccctcc agtcgaccgg gccggcgctg ccgtacacgc caccgcccag ctcccgccag
    10441 gtggtcggga tgatgaaccg ctccacctcc ggccgcgtcg agccgaagaa gatcggcggc
    10501 tcgtgcagct cgttgatgaa cccgaccggc acgagcagga gtccgccgcg ccccccgaag
    10561 gcgtcgttgt ggcggtaatc gacgtaggcg aactcgaccg cgacctcgcc gtcggcgccg
    10621 tccgcggcga gcgcgtgctc gacctcgatc tccgagttga acaggagctt gtcgttgaac
    10681 ttgtagccga agtagaggac ggcccgcagc agatcggcgc tggcggtctc ccccgaggcg
    10741 gcgccgtcgt cctgcgaatc gtccgggtcc gtgtagatga actcgccgta gccgccgatg
    10801 gcgaccccgc gcttcgtctg gtagaccttc gaagccgccg ggccgaagcc gccgacgctc
    10861 ttgtccgctt ccggcgccgc cgcctcgccg aggcgcagcc gctcgatctc cttcgagagg
    10921 gcctcgatcc gcttctccag atcggccgca tcggccgcgg gcacggcggc gccgcgcccg
    10981 cgcgccatct ccgccttgag ggccgacacc tgctcttcga ggtcccggag tctccgggcc
    11041 aggtcgtcgg tcgcggccga ctctcccgcg gcggccgcct ggcctgcggc cggttcggcc
    11101 gcgacggcgg ccccggaggc ggcggcgagg agggcgatcg tcatccacgt ccggagcgag
    11161 cgtgcgatcc ggggcgcggc ccttcgggcg gacatcggtt ccatgcgtcg gactcccttc
    11221 acgagtgcgg ttcgcccggc tgcccggggg actgtgtggt tggaaacgaa tcgccgggcg
    11281 cggagccggc gagggccccg cgaccgcgac gaacgagcgc tccgtcgcga tcgcggcgga
    11341 ggtacaggac atcgagacgg tgatcggcgg cgtacgcgag gccgcgatcc ggccccatga
    11401 cgaagagggc cgtggacagc gcgtcggcgg aggtggcgtc gggggccacg acggtcaggc
    11461 tgccgtcgaa gcgggcggcg gcgccgagcc gcggatccag gaggtgggat cgcgggccgt
    11521 ccggcccgcc cgccgcgcgc tcggagtttc cggaggtggc gatcgatccg gcccgcagca
    11581 cgaaggtgcc gacgaggtga tcgcggtccc gcggatccga cagcccgatg atccagcctc
    11641 cgtcgtccgg accccgcccg aacaccagga cctggccgcc gaagtcgagg cgcgccgccg
    11701 agacgccggc ccccttgagg attccggccg ccgcgtcgag cgcgatcccc ttcccgatgc
    11761 cgccgaagtc gatcccgacg ccgggcgcca ggaagcgcac gctcttcgcg tcgggacgga
    11821 tctccaccag gcgccagccg atcggatcgg gcccgggtcc ctgtggggct tccggcgccg
    11881 cgccacggag ccggccctcg tcgccgcgca gtccgagccg cctcaccagc ggctccaccg
    11941 tcgggtcgaa cgctccgtcg gtcttctccg cccacgtgag tgagccggcg accgcgccga
    12001 acaggtcggc cgagcacgtc accgcaccct ccgcggccgc ccggttgagg agggcgatct
    12061 cgctgtcgtc ccgccagttc gagagaacct ggtcgagccg ttccacttcg ccgaacgcgc
    12121 ggtcgatcga ttcctccgcg cggggccccg acgcctcgat cacgagccgg gtccccatga
    12181 ggaagcgcgc ccgctccacc ggcgccgtcg cggggtccgc gcccggaccc ggtccggcgg
    12241 ccggcaccac caggcacgcc gccaccaccg ccgtccaccg ccaccgcgtc cgcacgcccg
    12301 ccccctctcc gtccgcttgt tgcaattgcg actcagtctc aataagcgga cgcactctag
    12361 cgcccgtgcg aggctcgtgt caagcggtcc ccgcgtcgcc gcgcgcccat gcgatactcc
    12421 ccgcccggcg cacgacggcg ccgtgcggag gacggaagat ggcggagcag ccccggccgg
    12481 tcagcgcgtc gcgcgtcgcg atgaccgaga tcgtcatgcc cgaggacacc aatccctacg
    12541 gcaacgtgta cggcggccgt gtcatggcac tcatcgacaa ggcggcggcg atcgccgcga
    12601 tgcgccactg ccgggccaac gtggtgaccg cgctcgtcga ttccctcgag ttccgcggcc
    12661 cggtccggct cggccacatc ctgaggctgc acgcggaggt ccacgcggtg ttcgggacct
    12721 ccctggacgt cggcgtggag gtgacgtcgg aggatccgct gaccggacgg gtcgcgcact
    12781 gctgcagcgc ctcggtcctc atggtgtcgc tcgacgcgcg cgggcgtccg gcgcaggtcc
    12841 cgcgcctcaa gccgcgcggc gccgtcgagc gcgagcggga gcgggcggcc cggagccgcc
    12901 ggcgcgcccg cctgcgcagg cgcggtcctc gcccgcccgc gtccgccgcg tcagccgtct
    12961 gacatctctt cgtcggcgct ttgcgctccg gcaccgcccg ccccatattc ggacggccga
    13021 tggagatcgt ccggtgagcg caccgaaagt cctcggcgcc gcgcccctcc cccacccggg
    13081 agggggggac gagcgcctgc tcgaactcat gcagatcgcc cgctcgatca cctcgtcgct
    13141 gcgcctcgac gaggtgctcg gacgggtgat cgacggcgcg gtgcgctttt cgggagcgca
    13201 gcgcggcttc ctgttcctca aatcggccga cgggcggctg gtccgctggc cggctccgcc
    13261 ggacggagcg ccgccggtcg aggtgagcct gtcggtggcg gaggaggtcg cgcgcagcgg
    13321 ccgcccggtg caccgcgacc acctcgccgg ctccgacacc gccagcctga cggacagcat
    13381 cgtgcgcctg cggctcatgg cgatcctgtg cgtcccgctc ctggcgcagt cggaggtcat
    13441 cggcgtcatc tatctcgaca gccgccgccc gatgccgcac caggcgcacg atctcgatct
    13501 gctcgaggcg ctcgccgggc tcgccgccat cgccatccag aacagccgcc tggtggagga
    13561 gcgtctccgg gccgagcgca cgctcgctct cgggcggacc gcccgcgctc tcgtccacga
    13621 tctccgcagc cctctgacgg cgattcgcgc gctcgccgag ctcctgcacg ggcgcgccgc
    13681 cggcgacgcg gcgacgcgcc gccacctggc gtcgatcatg gacgaggcgg atcgcctcgc
    13741 ggagctggcc tccgatctgc tccgcttcgc cggaggggcg ccgccgctac ggcgcgaggc
    13801 ggtggtgctg gggccctacc tgacgcggct gctggagccg ctgcggccac gcctggagtc
    13861 gtccggcgtc gcgctcgtga ccgacctcga gccgaccgcg gtggccccgc tcgacgcgcc
    13921 gcgcgcggcg cgcgtcgtcc acaacctggt ggcgaacgcg ctcgacgcga tgccggacgg
    13981 cggaaccctg accgtcgcct gcggcacgac cggcgggcgc gcgtggctgc gcatcggcga
    14041 ctccggctgc ggcatgagcg aggcggtccg gtcgcggctg ttcgagccgt tcttcaccca
    14101 cggcaaggag cacggcaccg gcctcgggct ggcgctggcg cgggccatcg tcgaggagca
    14161 cggcggccgc atcgaggtcg agagcgcccc gggacgcggc agcgtcttca ccatcgagct
    14221 cgaggcggcc ccctccgcct aacgctcgac gacgaggacg tcgtccggac ccatctgcgg
    14281 atccccctcg agcgcgagtc tccccggatc gattccgccg agggcgcgcg ccatctcggg
    14341 aatcagcggc cgcagccccg ccgcggcctc cggagccagt cgcgccacga ccgtcccttc
    14401 gggccgcgcc ccgaggcgcc gcgcctggcg gacgatctcc tgctggagcg tcggaagtga
    14461 tcgcacgcgg ccggcgccgt cgcaggcggg gcaggcgcgc aggagctgcc ggtcgaggct
    14521 gggacggctg cgctggcggg tgatctccac gagcccgaac tcgctcatcg gcagcatgcg
    14581 gctgcgcgcc cggtcccgct gcagccgttc ggcgagcgcg gtccggaccg cctcgcggct
    14641 ctccggccgc tgcatgtcga tgaagtcgat gacgatgagc ccgccgagat cgcgcaggcg
    14701 gatctgccgg gcgatctcct tcaccgcctc caggttggtg cgcagaagcg tctcctcgag
    14761 atcgccgcgc cccacgtact tgccggtatt cacgtccacc gcgacgagcg cctcggtcgg
    14821 gtggatggcg atgtggccgc cggacgggag ccagacacgg gccttgaggg cccggtcgat
    14881 ctgcgtggtg agccggcgcg cgtcgaagag gggttccggc ccttcgtggc gcacgatacg
    14941 cggccccgcc gccccaagcc gcgccagcgc ccgccgcacc tcctcgaggg cgtcgtcgga
    15001 gtcgacggcg acctcctcga ccccgtggcg cagctgctcc cgcaggaccc gctcgatgac
    15061 cccggcctcc tcggagagga gcgccggcgc ccgcgcctcg gcgcgccgcg cctggatccc
    15121 gcgccacgtc tccgccagga ggagaatctc ctcctgaagt gcggcggggt ccgcctcggc
    15181 gccggcggtg cgcaggatga gcccccccgg cggccgcgtc ctctcgtgga gatcgcgcag
    15241 cagctcccgc agtcgcagcc gctcctcctc ggaggcgatc cggcgcgaca ccccgagatg
    15301 ctcgacggtg gggaggagga cgacgagccg ccccggcatc gacaggtggg cggtgacgcg
    15361 cggacccttg cgcgccagcg gatccttcgc cacctgaagc acgatctcct ccccttcccg
    15421 cacgcgttcc tcgatgcgcg gcgcgggagc cgccggcccg ggcggcgccg cgtcggtgtc
    15481 gtcgccggcc cactcgggcc ggaggcgcgc cgggccgggg gcgtcgtcga cgtgcaggaa
    15541 cccgtcccgt tccagcccca ggtccacgaa cgccgcctgc atgccgggca ggacgttgcg
    15601 cacccggccg aggtagacgt tccccgcgac cgggcgtgcc ccggcgcgct cgacgtgcag
    15661 ctcgaccagg agatcgtcct cgatcagggc gacgcggcgt tctccgggga ggctgtcgat
    15721 gaccaggcgg gacgtcaacc tcagttcacc agccggcgca tgtggacgtt caggaccagc
    15781 cccagcccga ggagggtgct gacgagacag gagccgccgt agctcaggag aggcagcggc
    15841 acgccggtgg tcggcaggac gccgagcacg acgccgatgt tgatgagcgc ctggcccccg
    15901 aagacgcacg ccacccccat ggcgaggaac gccccgaggc gatcgcgcgc gacccgcgcc
    15961 gtcgagacgc agcgggtcag gacgatgaag tagagggcga gcacgagcgc cgagccgagg
    16021 aatccccgtt cctccgccag gaccgcgagc acgaagtcgg tgtgctgctc cggcaggaac
    16081 tgcagcgacc cctgcgtccc cgagaggaat ccctt
//
LOCUS       DASRIQ010000162         9178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1872137, whole genome shotgun sequence.
ACCESSION   DASRIQ010000162 DASRIQ010000000
VERSION     DASRIQ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872137"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1459)
                     /locus_tag="VFD06_03945"
     CDS             complement(83..1459)
                     /locus_tag="VFD06_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02722.1"
                     /translation="MFGDYSQPNNHILHTAAVRVSLLAFGPSELALRLPALAGYVLLL
                     VAVALLARQLLRYATARVLCLAAIALHPYMIDFGALARGYTLGLGLALLGLVCAVRAA
                     RSPTRQVALGWLAVAGLCVGGAVGAVMLFVNLIAAEVCAAFVVLGRERPWRERALGLG
                     AVILPALAVLAATYGHVLRVPGRLQFGALDLTTSLASLHRLFVYDTQSLLDAHGHPLV
                     SGPIVPWQDGILSAAFGPLLAAPSVGVVLGVLVAAAIASAVVRAGSRERNAAALPGLM
                     LAGVFGLAAAEHAMAGLPWPFHRTWIAAVPLAVLALAVATEALLEAFPPERRRAAGAA
                     LGAACALFIAVGCTRWNPSIYREWPDNAPVREVLDRIAAARAPGGSTSVGHPWYLDAC
                     FRYYREVRHLDWLELAPRPVLAEQPGAQFVLANRRTDMGQFAEYGALARWESLGVTLL
                     QRKGGEAP"
     gene            1775..5950
                     /locus_tag="VFD06_03950"
     CDS             1775..5950
                     /locus_tag="VFD06_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZN02723.1"
                     /translation="MRLVRSPVWIAALLALGAAAPAHRLTFEDRVRDLETLERFRYAH
                     QIGARRPFEEAVPRALLEKKVRTTLQESAVLASLWNTPIQGDRLRRELERIAAETRMP
                     DRLREMYALLGNDPLRIQESLARPTLADRLARNFFAYDERIHAPSRREAERLREDLAS
                     GRLNPRAAHPRRDTVEMMRVEPAEPAVSPEETLPPAGDGTSRAFRLRLSPEDFEQRRT
                     GLPARTGVVGPLRENRDDFVVQVVLDASPDSITVANYVTPKRSWDEWWAGVASRLDER
                     ALAEEVPAAGPLPLPRSDASAGGPASSCPAGNEWDSMLKRGDSGVDAPLRRYGHPVVW
                     TGNLMIVWGGQGQNAPQGSGGRYDPATDTWMPVSTIGAPSSRTGHSAVWTGGSMIVWG
                     GTDFSGATNTGARYDPATDTWIPLSAIDAPAARSGHSAVWTGTEMIVWGGTASASVFL
                     QTGGRYDPAADSWKTNSVTGAPSGRLDQTAVWTGSLMIVWGGFDGASSLLTGGRYDPV
                     ADTWSPTSISGLRGRRGHTAVWAGSVMIVWGGEDASGVMDDGAYYEPASDFWGGVSTI
                     GAPSGRSRHTAVWSGTSMLVWGGTSGPGSRLKTGGRYDPFADAWTAIGPTNAPEGRLR
                     HSAVWAGDQMIVWGGTDLLDALSSGGRYDPGGDGWTPTARDNAPWGRSGHSAVWTGNH
                     MIVWGGFGQDGPFIQGTLHDGARYDPVLDAWTPLANGFLFGRSGHAAVWTGDRMIIWG
                     GNAGSLSSASTGSRYDPIEDVWAPTSMTNAPPPRFIPTVVWSGVEMIVWGGAAPSNVN
                     TGGRYNPLTDTWSPTSTAANVPQARSNHSAVWAGSVMVVWGGYVFDPITRQTIAVNTG
                     GRYDPVTNTWTPTAIASAPTPRRLHTAVWTGDRMIVWGGSDTVSQLPNGGRYDPVTNA
                     WTTMATPGVASEALTKTSLWTGERMLTWGGFDPATLSPTGARYDPVADTWSSLCRRNA
                     PSARNSYTAVWTGTQMIVWGGQPQSGFAYLGDGGRYTSGPGVDADNDCVTECQGDCDD
                     AVATVYPGAPELCDDLDNDCDGNKDGFATSCGVGSCASAGLCLSGVDTCVPGSPAPEI
                     CDGLDNNCDGVVDNPADDDHDGLDECHGDCDDANFAVRPGRPEQCDNLDNDCSGTIDG
                     FPTICGIGACSSAGTCIAGVNVCVPGEPSAEVCDGIDNDCDGVVDDAPIPAGTPLVAI
                     DPSVTAPTLAWEALSDATSYDVVFGDVGTLIATGGDFTAAVQGCVAFHLGGTSLPLGP
                     APAPGNAIFYLVRGVNCGGRGTYDSGGPGQVGSRDVEIDASPLGCGQCGDGACTGAET
                     CGSCIADCGYCPFCTTDEDCQAADCCPTTFCIFVWETQSCSGDACGGCTFPQIGCGTY
                     CGCNEGACMLTFY"
     gene            complement(5993..7855)
                     /locus_tag="VFD06_03955"
     CDS             complement(5993..7855)
                     /locus_tag="VFD06_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN02724.1"
                     /translation="MRDTRVLPAVVALCALLLLPVSAWSDQGGDQPKTVFCESIPAPA
                     TGACTVTTGDSALRLKGVVLAVDTVFTGGEVLVDPSGLIQYVGCSAARPAALTSMAMS
                     ATKIDCPQGVISPGLINTHDHLSFDHNAPFPATGVRYDHRNDWRPTSGVAENRSQARM
                     AWSELRQLIAGTTTITGASGVVGFIRNLDLRGFPAFDDLLWNSFAGEPPRLISPDTFP
                     LEHGQDFTENVGDCSLYPLYPDFAASASSSDVYLPHIAEGINPAAANEFDCLSSPERH
                     GLDITNDRLVMIHGIALDAHDGRTAANDRASLVWSPRSNLSLYGNTAPVRMLRNQGVL
                     MTIGTDWTPSGSSTLPRELSCAAEFNGTYLDQAFSDRELWLMATYNPALALKVGDKIG
                     SLKPGLFGDIAVYDGGSSANPYRAVLEATARTTLLVLRRSSLPFPFVNGPFYIGSIAE
                     FGDAGILSSLPPTLHDIVAPTYGVTAPLCEPISVCGASKKICPLRETWWLPLAGLGNR
                     LTLASLGVANGSSYELFSCGTPTGEPTCTPSRPAEYDGARTKVDSDGDGVVDAQDNCR
                     KTFNPVRPMDGGTQPDADGDGRGDACDKCPLDQGPLCTAIDPYTGETVIITDGE"
     gene            complement(7924..8979)
                     /locus_tag="VFD06_03960"
     CDS             complement(7924..8979)
                     /locus_tag="VFD06_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02725.1"
                     /translation="MTRQTKRWAVVAGAGSTVLLLAGAMKSLPAQSANFELVSTIVFS
                     STRDTPNAATQADIVNAAEIYLMDPDATNPRRLTENADGDGFAALSPDGKKIVFNSNR
                     LRPAEGPFNTADLFVMDADGDIEVHLIRGSSATWSPDGKDIAFHASASGTGLPIKPDP
                     GAATSDSDIFVMNVDDSLDGSPEPVNITNSPLAIDDDPDWSPDGQTIIFTSHLASDNP
                     INSATAEIMAISADGSGGPLPLTSNTEEERAPSWSHDGARIVFMCRRGGPDFEICVMN
                     ADGTDQVQLTSNSAPDATPTFSPDDQTIVFHRQLGPTQQIWVMNADGTGQTQMTSPPG
                     FNTFANWGELRVRVSQP"
BASE COUNT         1485 a         3144 c         3124 g         1425 t
ORIGIN      
        1 gtgacagagc cgcaggagcc gacgcaaccg tgaccacctg acatgcgcgc cctgtccgga
       61 ccgagcgcgc gtggggccgg cgtcatggcg cctccccgcc tttgcgctgc aagagcgtga
      121 cgccgaggga ctcccatcgc gcgagcgcgc catactcggc gaactggccc atgtccgtgc
      181 gccgattcgc gaggacgaac tgcgcgcccg gctgctccgc gagcacgggc ctgggagcca
      241 gctcgagcca atcgaggtgc cgcacctcgc ggtagtagcg aaagcacgcg tcgaggtacc
      301 acgggtgtcc cacgctcgtc gaaccgcccg gagcacgcgc ggcggcaatc cgatcgagca
      361 cctcgcgcac cggcgcattg tctggccact cgcggtagat gctcgggttc cagcgagtgc
      421 acccgacagc gatgaacagc gcgcatgcgg ccccgagcgc cgcgccggca gcgcgccggc
      481 gctcgggcgg gaacgcctcg agcagggcct ccgtcgccac cgcaagcgcg agcactgcga
      541 gagggacggc cgcaatccag gtgcgatgga acggccaggg gagcccggcc atcgcgtgct
      601 ccgccgcggc gagaccgaag acgccagcga gcatcagacc cggaagggcg gccgcgttgc
      661 gctcccggct cccggcgcgc acgacggcgg atgcgatcgc ggcggcgacc agcacgccaa
      721 gcaccacgcc gaccgagggc gcagcgagaa gcgggccaaa tgccgcggac aggatgccgt
      781 cctgccacgg gacaatcggt ccgctcacga gcggatgccc gtgcgcgtcc aggagcgact
      841 gtgtgtcgta cacgaagagc cggtgcagcg aggccaggct ggtcgtcagg tcgagagcgc
      901 cgaactgcaa ccgtccgggc acccggagga catgcccgta cgtcgccgcg agcaccgcga
      961 gcgcgggcag gatcaccgcc ccgagcccca gcgcacgttc ccgccacggc cgttcacgcc
     1021 cgaggacgac gaacgcggcg cacacctccg ccgcaatgag gttcacgaag agcatcacgg
     1081 caccgaccgc tccccccaca cacagccccg ccacggcgag ccagccgagg gcgacttggc
     1141 gggtcggact ccgcgccgcg cgcaccgcgc acacgagccc cagcagagcg agccccaggc
     1201 cgagggtgta accgcgcgcg agggcgccga aatcgatcat gtacggatgc agcgcgatgg
     1261 cggccagaca caacacgcgc gccgtcgcgt accgcagcag ctgacgcgcg agcagcgcga
     1321 ccgcgaccag cagcaagacg taccccgcga gcgcgggcag ccgcagcgcg agctcactcg
     1381 ggccgaacgc gagaagggac acgcggacgg ccgcggtgtg caggatgtga ttgttcggct
     1441 ggctgtagtc gccgaacacg cgggggaaat cgtgcgaggc ccagccattg aaattccagg
     1501 cctcgtcggt cgtgatcggc gcgagtgccg ccttggcgaa agtcagcgcg aagccgaggg
     1561 cgacgacgag cgccgtggcg accccctcga gccgcagtgt ccgcccgccg tcgagtcttt
     1621 cagtccatgc aagcatcgac ctcgatctcg ccgcggttgt agaccagatc gcccgcttcg
     1681 ttcatcgctg agcagaaaag agaggtgcat gttacgcccg gttggcgtag actcgcccaa
     1741 aatccggttc aagaccgtgc aggcggaggt gggtatgcga ctcgtccgct ctcctgtctg
     1801 gatcgccgct ctcctcgccc tcggagccgc cgccccggcg catcggctca cgttcgagga
     1861 tcgcgtccgc gacctggaaa ctctcgaacg cttccgttac gcccaccaga tcggcgcccg
     1921 gcggcccttc gaggaggcgg tgccgcgggc cctcctggag aagaaggtca ggacgactct
     1981 ccaggagtcg gccgtcctcg cgtcgctctg gaacaccccg atccagggcg atcggctgcg
     2041 ccgggaactg gagcgcatcg ccgcggagac ccgcatgccc gaccggctgc gcgagatgta
     2101 cgcgctcctg gggaacgacc cgctccgaat ccaggaatca ctcgcgcgcc ccacgctggc
     2161 cgaccggctg gcgcggaact tcttcgccta tgacgagcgg atccacgcgc catcgcgccg
     2221 ggaagccgag cgtctccgag aggacctcgc ctccggacgc ctgaatcccc gcgccgcgca
     2281 tcctcgcagg gacacggtcg agatgatgcg ggtcgagccg gcggagcccg ccgtctcacc
     2341 cgaggagacc ttgccgccgg ccggggatgg gacttcacgg gcgttccgct tgcgcctttc
     2401 cccggaggac ttcgagcagc gtcggaccgg gcttccagcg cgcaccggtg tcgtcggacc
     2461 gctgcgggag aatcgggacg acttcgtcgt ccaggtcgtc ctcgacgcga gcccggacag
     2521 catcacggtc gccaattacg tcacgccgaa acggtcgtgg gacgagtggt gggcgggcgt
     2581 cgcgagccgg ctcgacgaaa gggctctcgc cgaggaggtg cccgcagcag ggcccctccc
     2641 gctcccccga tccgacgcct ccgccggcgg cccggcctcg agctgcccgg ccgggaatga
     2701 gtgggattca atgttgaaga gaggcgactc cggcgtagat gcccccctcc gccgctatgg
     2761 ccaccccgtc gtctggaccg ggaacctcat gatcgtgtgg ggaggccagg gccaaaacgc
     2821 gccgcaaggc agcggcggcc gctacgaccc cgcgaccgac acctggatgc cggtctccac
     2881 gatcggggct ccctcgtccc gcaccggcca cagcgccgtc tggaccggcg gctccatgat
     2941 cgtgtgggga ggaacggatt tcagcggtgc gaccaacacc ggtgcccggt acgatcccgc
     3001 gaccgatacc tggatcccgc tgtccgccat cgacgcacct gccgcccgct ccggtcactc
     3061 cgccgtctgg accgggacgg agatgatcgt ctggggcggg acggcctccg catccgtctt
     3121 cctccagacc ggcggacgat acgaccccgc ggccgattcg tggaagacca actccgtcac
     3181 cggggcccct tcgggtcgcc tcgaccagac ggcggtctgg accggaagcc tgatgatcgt
     3241 ctggggcggg ttcgatggcg cgtccagcct cctgaccggc ggccgctacg atccagtcgc
     3301 cgacacctgg tcccccacga gcatctcggg tctccgcggt cgccgcgggc acactgccgt
     3361 ctgggcgggc agcgtgatga tcgtctgggg cggcgaggat gcgtccggcg tcatggacga
     3421 cggcgcgtac tacgagccgg cgtccgattt ctggggcggg gtctcgacga tcggcgcacc
     3481 gtccggacgt tcgcgacaca ccgcggtctg gtccgggacg tccatgctcg tctggggggg
     3541 gacctcgggc cccggttcga ggctcaagac cggaggccgc tacgatccgt tcgcggacgc
     3601 ctggacagcc atcggcccca cgaacgctcc ggaggggcgc cttcgtcaca gtgcggtctg
     3661 ggcgggggac cagatgatcg tgtggggcgg caccgaccta ctcgatgccc tcagcagcgg
     3721 gggccgctac gaccccgggg gcgacggatg gacgccgacc gccagagaca acgccccctg
     3781 gggccgctcc ggccattcgg ccgtctggac cgggaaccac atgatcgtct gggggggctt
     3841 cggccaggac ggaccgttca tccagggcac gcttcacgac ggcgcccgct acgacccggt
     3901 cctggatgcc tggacgcccc tcgccaacgg gttcctcttc ggacggtccg ggcatgccgc
     3961 cgtctggacg ggcgatcgca tgatcatctg gggaggcaat gccggctcac tgtcctctgc
     4021 gagcaccggc tcccgctacg accccatcga ggatgtctgg gctccgactt cgatgacgaa
     4081 cgcccctccg ccccgcttca ttcccaccgt tgtctggtcc ggggtcgaaa tgatcgtctg
     4141 gggcggagcc gcgccgagca acgtcaatac cggaggtcgc tacaatccct tgaccgatac
     4201 ctggagtccg acctcgacgg cggcgaatgt tccgcaggcg cgctccaatc attccgcggt
     4261 gtgggcgggg agcgtcatgg tcgtctgggg cgggtacgtc ttcgacccga tcacgcggca
     4321 aacgatcgcc gtcaacaccg gcggccgcta cgacccggtc acgaacacct ggacgccgac
     4381 cgcgatcgcc agcgccccca cccctcgccg gttgcacact gccgtctgga ccggggatcg
     4441 catgatcgtc tggggcggtt ccgacaccgt ctcacagctc cccaacggcg gaaggtacga
     4501 ccctgtcacg aatgcctgga ccaccatggc gacccccggc gtcgcctcgg aagccctcac
     4561 gaagacctcg ctctggacgg gagagcgcat gctcacctgg ggaggcttcg accctgcgac
     4621 tctctcaccg accggcgctc ggtacgatcc ggtcgccgat acctggtcct ccctgtgtcg
     4681 gcgcaacgcc ccctccgcac gtaacagcta caccgccgtg tggaccggga cgcagatgat
     4741 cgtctggggc ggccagcctc agtcggggtt cgcctacctc ggcgacgggg gccgctacac
     4801 ctccgggccc ggggtggatg ccgacaacga ctgcgtcacc gagtgccagg gggactgcga
     4861 tgacgctgtc gccaccgtct acccgggggc gcccgagctc tgcgatgatc tcgacaacga
     4921 ctgcgacggc aacaaggacg gtttcgcgac ctcctgcggc gtggggtcgt gcgccagcgc
     4981 cgggctctgc ctctctggcg tcgatacgtg cgtccccggg tcccctgctc cggagatctg
     5041 cgacggtctc gacaacaact gcgatggcgt cgtggacaat ccggcggacg acgaccacga
     5101 tggtctcgac gaatgtcacg gggactgcga cgacgcgaac ttcgcggtcc ggccggggag
     5161 gccggagcag tgcgacaacc tggacaacga ctgcagcggg acgatcgacg gcttcccgac
     5221 aatttgcgga ataggcgcct gcagcagcgc cggaacctgc atcgccggcg tgaacgtgtg
     5281 tgttcccgga gaaccctcgg ccgaggtctg tgacgggatc gacaatgact gcgacggggt
     5341 cgtggacgat gcaccgatcc ccgccggaac gccgttggtc gccatcgacc cgtcagtcac
     5401 ggcgccgacg ctcgcctggg aggcgctgtc cgacgcgacc tcctacgacg tcgtcttcgg
     5461 cgacgtgggg accttgatcg cgaccggcgg cgatttcacg gcggccgttc agggctgtgt
     5521 cgcgttccac ctcggcggaa ccagcctgcc gctcggcccg gctcccgctc ctgggaacgc
     5581 catcttctac ctggtgcgcg gggtcaactg tggcggccgc gggacgtacg attcgggagg
     5641 gccgggccag gtcggctcgc gcgacgtcga aatcgacgcg tcaccgctgg gctgcggcca
     5701 gtgcggcgat ggtgcctgca ccggcgcgga aacctgcggt tcctgcatcg ccgactgcgg
     5761 ttattgcccg ttctgcacga ccgacgagga ctgccaggcc gcggactgct gtcccaccac
     5821 gttctgcatc ttcgtctggg agacgcaaag ctgtagcggt gacgcgtgcg gcggctgtac
     5881 gttcccgcag atcggctgcg gaacctactg cggctgcaac gaaggagcct gcatgctcac
     5941 cttctactga ggggcgacgc cgcttgcggc gaacggggtc acgcacagcg gcctattccc
     6001 cgtcggtgat gatgaccgtt tctccagtgt acggatcgat ggcggtgcag agcggcccct
     6061 gatccagcgg gcacttgtca cacgcgtccc cgcggccgtc tccatcggca tccggttggg
     6121 tccctccatc catcggcctg acgggattga aggttttccg gcaattgtcc tgagcgtcca
     6181 cgacgccgtc gccgtcggaa tcgaccttgg tgcgggcccc gtcgtattcc gccggccgcg
     6241 agggagtaca ggtgggttca ccggtcggcg tgccgcacga gaacagctca tacgagcttc
     6301 cattcgccac cccaaggctc gcaagagtca gacggttccc caatccggcc agcggaagcc
     6361 accaggtctc ccgcaaggga cagatcttct tggaggctcc gcacacgctg atcggctcgc
     6421 agagaggcgc cgtcacaccg taggtcggcg ccacgatgtc atggagtgtc ggcggcaggg
     6481 agctcaggat gccggcatca ccgaactccg cgatgctccc gatgtagaac ggtccattga
     6541 cgaaaggaaa cggcagcgag gatcgtcgca ggacgagcag tgtcgtcctg gcggtcgctt
     6601 cgagaaccgc ccggtagggg ttggcggacg agcctccgtc gtagacggcg atgtcgccga
     6661 agaggcccgg cttcaacgaa ccgatcttgt cgccgacctt caaggcgagg gcgggattgt
     6721 aggtggccat gagccacagc tcccgatccg agaacgcctg atcgaggtac gtcccgttga
     6781 attcggccgc gcagctcagc tcgcgcggca gggtcgatga gcccgagggc gtccagtcgg
     6841 tcccgatcgt catcaggacc ccctggttcc tcagcatccg caccggcgcg gtgttcccgt
     6901 agagcgacag gttcgacctc ggggaccaga ccagcgaagc gcgatcgttg gccgcggtcc
     6961 ttccgtcgtg cgcgtccagc gcgatgccgt ggatcatgac gagcctgtcg ttcgtgatgt
     7021 ccagtccgtg tcgctccggg gagctcaggc agtcgaactc gttcgcggcc gcgggattga
     7081 ttccctccgc gatgtggggg aggtagacgt cggaggagga cgcggaggcg gcgaagtcag
     7141 ggtagagagg gtagagcgag cagtctccga cgttctccgt gaagtcctgg ccatgttcga
     7201 gaggaaaggt gtccggggaa atgagccgcg gcggctcgcc ggcgaacgag ttccagagca
     7261 ggtcgtcaaa cgccgggaat ccgcgaagat ccaggttcct gatgaagccg acgactcccg
     7321 aggcgccggt gatggtcgtg gttcctgcga tgagctgccg cagctccgac caggccatcc
     7381 tggcctgcga gcggttttcc gcgacgccgg atgtcggacg ccagtcgttg cgatggtcgt
     7441 accgcacacc cgtcgcgggg aacggagcgt tgtggtcgaa cgacaggtga tcgtgcgtgt
     7501 tgatgagtcc cggggagatc accccctgcg ggcaatcgat cttcgtggcc gacatcgcca
     7561 tggaggtcag cgctgccgga cgcgccgcgc tgcacccgac gtactgaatc agccccgagg
     7621 gatcgacgag aacctctccg cccgtgaaga cggtgtcgac ggccaggacg acgcccttga
     7681 gccggagggc gctatctccc gtggtgacag tgcacgcgcc cgtggccggc gccgggatcg
     7741 attcgcagaa caccgtcttg ggctggtcgc cgccctgatc gctccaggca ctcacaggaa
     7801 gaagcagcag agcgcacaat gcgacgacgg ccggaaggac tcgcgtgtct ctcatgaaga
     7861 cctccgattg atgaaagacg gagatgggaa gcctggtcag tcgcatggag tcctccggcg
     7921 tcttcagggc tgtgacacgc ggacccgcag ctcgccccag ttcgcgaacg tgttgaaccc
     7981 gggaggactg gtcatctgcg tctgacccgt cccgtcggcg ttcatcaccc agatctgctg
     8041 ggtcggtccc agctgccgat ggaagacgat cgtctggtca tccggagaga acgtgggggt
     8101 cgcgtcgggc gccgaattgc tggtgagctg gacctgatcc gtcccatcgg cgttcatgac
     8161 gcagatctcg aagtcgggac cgcccctccg gcacatgaac acgatccggg cgccatcgtg
     8221 ggaccaggag ggcgcgcgtt cctcttcggt gttgctggtc agcggcagcg gcccgccgct
     8281 gccatccgcg ctgattgcca tgatctcggc ggtggcggaa ttgatcggat tgtcggaggc
     8341 gagatggctc gtgaagatga tcgtctgccc atcgggcgac cagtccgggt cgtcgtcgat
     8401 cgccagcgga ctgttggtga tgttcaccgg ttccggcgac ccgtcgaggc tgtcgtccac
     8461 gttcatgacg aagatgtcgc tgtcgctcgt ggccgcgccc ggatcgggct tgatcggcag
     8521 ccctgtcccc gacgcggaag cgtgaaaggc gatgtccttg ccatccggag accaggttgc
     8581 cgagcttccc cggatgagat ggacctcgat gtccccatcg gcgtccatca cgaacaggtc
     8641 ggccgtgttg aagggtccct ccgcgggccg gagccggttg ctgttgaaca cgatcttctt
     8701 tccgtccggc gacagggccg cgaatccgtc accgtccgcg ttctccgtca accgcctcgg
     8761 attcgtggcg tccggatcca tgaggtagat ctccgccgcg ttgacgatgt cggcctgggt
     8821 ggcggcgttg ggagtgtccc gcgtgctcga aaacacgatc gtggacacga gttcgaagtt
     8881 tgcggattgc gccggcaggc ttttcatcgc gccagcgagc agcaggaccg tgctccccgc
     8941 tcccgccacg accgcccatc gtttcgtctg tctggtcatg ttcgcctcct gggatgcgtt
     9001 gtctggtttg gacgccggcg aagcctgcgg tcccgcgacg gcgcgcaggt gatctcgcgc
     9061 ccgcctgtgc cgtggtcttc ggtccgggag cccctctgtt tcataagaac gccagcaggg
     9121 cgctcgagcg cacgcgtccc ctctcggccg tggaaacgtt gggggccgag gtcagccc
//
LOCUS       DASRIQ010000163         5998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3722574, whole genome shotgun sequence.
ACCESSION   DASRIQ010000163 DASRIQ010000000
VERSION     DASRIQ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3722574"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFD06_03965"
     CDS             <1..229
                     /locus_tag="VFD06_03965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN02726.1"
                     /translation="EADGAGAVQVAERIRLSIESTEFTLDGETVRTTISAGVALFPAH
                     GNDLKSLVSRADRALYRAKEEGRNRVVLDIG"
     gene            complement(255..1511)
                     /locus_tag="VFD06_03970"
     CDS             complement(255..1511)
                     /locus_tag="VFD06_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HZN02727.1"
                     /translation="MPQNDEGPAPSGDRDALIHDWNRDGDAWRPPFKVMLDDETLRDG
                     LQGPSVVDPPIETKKGILRAMDRLGIDTADVGLPGAGPRAVADVTALCREIVDGRLTI
                     RPNCAARTMIRDIEPIVSISSSVGIPIEACLFIGSSAIRQYAEDWTLDMLLERTEEAV
                     TFAVKQGLPVMYVTEDTSRANPETLRRLYTRALACGATRLCVTDTVGHATPAGAAAIV
                     GFIKGVAAEHGSPGAGIDWHGHRDRGLDLPNVLAAVRAGATRVHGCGLGIGERAGNAP
                     MELILVNLRLLGWSDQDLSALPDYCRRVSEGCGMPIPHNYPVVGHDAFETGTGVHAAA
                     VIKALRKNDAWLADRVYSGVPASEVGRRQSIVIGPMSGKSNVVFWLEAHGIPSDDATV
                     TRVYEAAKKSDRVLQEQEIRSLIDRL"
     gene            1627..2376
                     /locus_tag="VFD06_03975"
     CDS             1627..2376
                     /locus_tag="VFD06_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02728.1"
                     /translation="MLALFLGVPQPAWALADWFGVELSALQTAPTGTAAIEDDGVPGT
                     EFDVENTLGVTDDDTSTQARVWFRWGKRNRLFVDYFNPNNEGEATLSQPLTFNGVTFN
                     AGESVDTSMEMELIQVKYRYHFLNLKVFEFGLGIGFNQVALNMDLTGSTTGTQVLDEK
                     VPFPTIAGGLVIKPLPGLHIRAEADWISISDGDDHADFLDYRAQIEWYFLHFFGVFGG
                     YRSVHCEVDTEDFGRADLTYEGPYAGLGVKF"
     gene            2469..3071
                     /locus_tag="VFD06_03980"
     CDS             2469..3071
                     /locus_tag="VFD06_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02729.1"
                     /translation="MAPRALPGRAIGRLDHPPFRPADAAEADRAAEAAARLSAPGTPE
                     HLAPRAAHQAAVLAIRMRAPLLVSGHDTFRDLIPLSSWSMNVPSAPADAALVRTLTIA
                     GDATARADEPNPEIVKVLVEGDERRLVALVRDRRRSARALGPDLFRRIAPRSAGLRGF
                     AVTLDLVPALAGSPEARRWLAGIDPTLALSLLRSAPAVFI"
     gene            complement(3212..4570)
                     /locus_tag="VFD06_03985"
     CDS             complement(3212..4570)
                     /locus_tag="VFD06_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN02730.1"
                     /translation="MTRSRIWGAVALAAALVVVGTAFNAARAGEEGKDKVHNKKVVIF
                     RSGGSWLGVQIADVDADRGKELGLRDVHGAEVQSVSPGSPAEESGIKEGDVITEYQGS
                     RIEGVAQLTRLVRETPSGRTANIEVWRNGASKDLQVTMKAREHDNDDLPGNVHVWSGD
                     ADGDGKMSWFHMPTPPEPPEPPDVDLQALEGLENLRVFGVPGMRPRLGITVDTVGKQL
                     ADYFGVKQGGGALVTSVGKGSAAETVGLKAGDVIVKVDDEAVQDAGDLHTAMRDRRGK
                     ALTLTVVRDRRETTIKVPEPPERETPPPGVRSRPAVDHGEIQRQIERAMRDGEQARRL
                     AMATQRAAYAEAMRAVHEAMERQYGTDTETPEAIEIDDEAIQDAIEDAHDEIEDAVDE
                     GIEQGLEGGADIQEIGTPTPDDEEGTVDAEDATEEQRAEIRRAVEEARRIRIQTQQED
                     DR"
     gene            complement(4584..5909)
                     /locus_tag="VFD06_03990"
     CDS             complement(4584..5909)
                     /locus_tag="VFD06_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02731.1"
                     /translation="MNPNPTVSAAPRLMTAIAGLLMALFTVRLALSVTAAPVRDPHVS
                     YYQEGRNMVHEIAGTIPAESARLKVDTHLGAVRVVRRPGGEVTYRVRVRALGPDMAEA
                     RRRLDRMVVGASREGDLLTFIGTLPKPADARGLGADFDIEIPESFGEVAVTTGAGDIR
                     AEGTPGKVTLLSRAGAIVARDLPGPLQAETRAGTITVSGMKGGARLISAGGDVLVDSG
                     DGDLFVRTSGGDVRIGRVGGRVEAETGGGSVAIERATAAVKVASNGGDIDIGDVGGEV
                     TAATGGGGIRVGRAAGGVRCETGAGSIVLNGVNGPMRALTSAGSIRALLAGRLPGDSD
                     LQTWHGDVIVAIPDSIPLTIRALVDNPVGHAIESDFPLTVLREVESTGRPLEIAETRI
                     GGGGAVLKLRTLGGRIVIRRAKALPGVPLPYPAPAPKQERRQAGEPRFE"
BASE COUNT          760 a         2218 c         2095 g          925 t
ORIGIN      
        1 tgaggccgac ggggccgggg cggtgcaggt ggcggagcgc atccgcctga gcatcgaatc
       61 gaccgagttc acgctcgacg gcgagacggt ccgcaccacc atcagcgccg gcgtcgctct
      121 cttcccggcg cacgggaacg atctcaagtc gctggtgtcg cgggccgacc gggcgctcta
      181 ccgcgccaaa gaggaaggac gcaaccgcgt cgtgctcgac atcggctagg gggccaggcg
      241 ccggcccagc gccgtcagag gcgatcgatg agggagcgga tctcctgctc ctgcaggacc
      301 cggtcgctct tcttggccgc ctcgtaaacg cgcgtcacgg tcgcgtcgtc ggaggggatt
      361 ccgtgggcct cgagccagaa gacgacgttg gacttccccg acatcgggcc gatgacgatg
      421 ctctgccgcc ggccgacctc ggaggcgggc acgccggaat aaacccggtc cgccagccag
      481 gcgtcgttct tccggagcgc cttgatcacc gccgcggcgt gcacgccggt gccggtctcg
      541 aaggcgtcgt gcccgacgac cgggtagttg tgcgggatcg gcataccgca tccctcggac
      601 acccgccggc agtagtccgg cagcgccgag aggtcctggt cgctccagcc gaggaggcgc
      661 aggttgacga ggatcagctc catcggggcg ttgccggccc gctcgccgat gccgagcccg
      721 cagccgtgca cccgcgtcgc gccagcccgc acggcggcga gcacgttcgg caggtcgagg
      781 ccgcggtcgc ggtggccgtg ccagtcgatt ccggcgccgg ggctgccgtg ctccgcggcg
      841 acgcccttga tgaagccgac gatcgccgcc gcccccgccg gggtggcgtg ccccaccgtg
      901 tcggtcacgc acaggcgcgt cgcgccgcag gcgagggctc tggtataaag ccgccgcagc
      961 gtctcggggt tcgcccggct cgtgtcctcg gtgacgtaca tcacagggag gccctgcttg
     1021 acggcgaagg tgaccgcctc ttcggtgcgc tcgagcagca tgtccagggt ccagtcttcg
     1081 gcatattgac ggatggcgga cgaaccgatg aacaggcagg cttcgatcgg aatcccaacg
     1141 gaggatgaga tgctcacgat cggctcgatg tcacggatca tggtgcgcgc ggcgcagttc
     1201 ggtcggatcg tcagccggcc gtcgacgatc tcccggcaga gggcggtgac gtccgccacc
     1261 gcccggggcc ccgcccccgg cagcccgacg tcggcggtgt cgatgccgag ccggtccatg
     1321 gcgcgcagga tccccttctt cgtctcgatc gggggatcga cgacgctcgg gccctgcagc
     1381 ccgtcgcgca gcgtctcgtc gtcgagcatg accttgaacg gcgggcgcca ggcgtcgccg
     1441 tcccggttcc agtcgtggat gagggcgtcg cggtcgccgc tcggcgccgg accctcgtcg
     1501 ttctgcggca tgaaaccgat gatagaaggg ggcgcgcgaa cgagtcaatg cgatggcgcg
     1561 ccgaatcggc gcgccggccg cggcgtccgg gcgccgcggg gccgatcggt tccggtcctc
     1621 gggctgatgc tcgcgctctt cctcggcgtg ccgcagccgg cctgggccct ggccgactgg
     1681 ttcggcgtcg agctgtcggc gctgcagacc gccccgaccg ggacggccgc gatcgaggac
     1741 gacggcgtgc ccggcaccga attcgatgtc gagaacacgc tcggcgtcac cgacgacgac
     1801 acctcgacgc aggcgcgcgt ctggttccgc tgggggaaga ggaaccggct gttcgtggac
     1861 tacttcaatc ccaacaacga aggggaggcg accctgtcgc agccgctcac cttcaacggc
     1921 gtcaccttca acgcgggcga gtcggtcgac acctcgatgg agatggagct gatccaggtg
     1981 aagtaccggt atcacttcct caatctcaag gtgttcgagt tcggcctcgg gatcgggttc
     2041 aaccaggtgg cgctcaacat ggatctgacc ggctcgacga ccggcactca ggtcctcgac
     2101 gagaaggtgc cgttcccgac gatcgcaggc gggctcgtca tcaagccgct gccggggctg
     2161 cacatccgcg ccgaggcgga ctggatctcc atcagcgatg gggacgacca cgccgacttc
     2221 ctcgactacc gggcgcagat cgagtggtac ttcctgcact tcttcggggt gttcggcggc
     2281 taccgcagcg tccactgcga ggtggacacg gaggacttcg gccgggccga cctgacctac
     2341 gagggcccct acgccggcct cggcgtgaag ttctgaagcg gcgggtcctg ctcctggccg
     2401 cgggggaggg ggcctcgcgg acggcggtcg actgggtcgc ggcgtcgcag gccgactgcg
     2461 acgtcgtcgt ggcgccgcgg gcgcttcccg ggcgcgcgat tggacgcctc gatcacccgc
     2521 cgttccggcc ggcggacgcc gcggaggcgg atcgcgccgc ggaggcggcc gcccgcctga
     2581 gcgcgcccgg cacgcccgag cacctcgcgc cgcgtgccgc gcaccaggcg gcggtcctgg
     2641 cgatccggat gcgggcgccg ctcctggtgt cggggcatga cacgttccgg gacctcatcc
     2701 cgctctcctc ctggtcgatg aacgtcccgt cggccccggc cgacgcggcg ctcgtccgca
     2761 cgctgacgat cgccggggac gcgacggcgc gcgccgacga gcccaacccg gaaatcgtga
     2821 aagtcctcgt cgagggggac gagcggcggc tggtcgcgct cgtgcgcgat cggcgccgct
     2881 ccgcccgagc tctcgggccg gacctcttcc ggcgcatcgc gccgcgcagc gccggcctgc
     2941 gcggcttcgc cgtgaccctc gacctcgtgc cggcgctggc ggggagcccg gaggcgcgcc
     3001 gctggctcgc ggggatcgat ccgacgctgg ccctttccct gcttcgcagc gcgccggcgg
     3061 tctttatcta gctcgagctg gaagaaaccg cggcccggcg ccccacgacg gagcgccggg
     3121 ccggccgtgc ctgggcggga tcagcgttca acaggcacgc acttcctggc gtgacggaga
     3181 ggtccggcgc gcgtgcgccg gagcgatcgc tctagcgatc gtcttcctgc tgcgtctgga
     3241 tgcggatgcg gcgggcctcc tcgacggcgc ggcggatctc ggcgcgctgc tcctcggtgg
     3301 cgtcctccgc gtccacggtc ccctcctcat cgtcgggggt cggcgtcccg atctcctgga
     3361 tgtcggcgcc gccctccagt ccctgctcga tcccttcgtc caccgcgtcc tcgatctcgt
     3421 cgtgcgcatc ctcgatcgcg tcctggatcg cctcgtcgtc gatctcgatc gcctccggcg
     3481 tctcggtgtc ggtcccgtac tggcgctcca tcgcctcgtg caccgcgcgc atcgcctcgg
     3541 cgtaggcggc ccgttgcgtc gccatcgcca ggcggcgcgc ctgctcgccg tcgcgcatcg
     3601 cccgctcgat ctggcgctgg atctcaccgt gatcgaccgc cggacggctg cgcacgccgg
     3661 gcggcggggt ctcccgctcc ggcggctccg ggaccttgat cgtcgtctcg cggcggtcgc
     3721 gcaccacggt gagcgtcagc gccttgccgc gccgatcgcg catcgccgtg tgcaggtcgc
     3781 cggcgtcctg gaccgcctcg tcgtccacct tgacgatgac gtcgcccgcc ttgagtccca
     3841 cggtttccgc cgcgctcccc ttgccgaccg aggtcacgag cgcgccgccc ccctgcttca
     3901 cgccgaagta gtcggccagc tgcttgccga ccgtgtccac cgtgatgccg agccgcggac
     3961 gcatgccggg gacaccgaaa acccggagat tctcgagccc ttcgagtgcc tgcaggtcga
     4021 cgtccggcgg ctcgggcggc tcgggcggag tgggcatgtg gaaccagctc atcttgccgt
     4081 caccgtcggc gtcgccgctc cagacgtgga cgttgccggg caagtcgtcg ttgtcgtgct
     4141 cgcgcgcctt catcgtcacc tggaggtcct tgctcgcgcc gttgcgccac acctcgatgt
     4201 tggcggtgcg gccggagggg gtctcgcgca ccaggcgggt cagctgcgcc acgccctcga
     4261 tgcggctccc ctggtactcg gtgatgacgt ccccttcctt gatgccggat tcctccgccg
     4321 ggctgccggg ggacaccgac tgcacctcgg cgccgtgcac gtcgcgcagg ccaagctcct
     4381 tgccgcggtc ggcgtcgacg tcggcgatct gcaccccgag ccacgaaccg ccggagcgga
     4441 agatcaccac cttcttgttg tgaaccttgt ccttcccttc ctctcccgcg cgcgcggcgt
     4501 tgaacgccgt ccccaccacc accagggcgg ccgccagcgc caccgctccc cagatgcgac
     4561 tgcgtgtcat cgttcctcct ccgtcactcg aaacgtggtt cgcccgcctg acgtctttct
     4621 tgcttcggag ccggcgccgg gtacggcagc ggcactcccg gcagcgcctt ggccctccgg
     4681 atcacgatgc gccctccgag cgtgcgcagc ttcagcaccg ccccgccacc cccgatgcgc
     4741 gtctcggcga tctccagcgg ccggccggtc gattccacct cgcgcaggac ggtcagcggg
     4801 aagtcgctct cgatcgcatg ccccaccgga ttgtccacga gggcccggat ggtgagcgga
     4861 atgctgtcgg ggatcgcgac gatcacgtcg ccatgccagg tctggagatc cgagtcgccc
     4921 ggcaggcgcc ccgcgaggag ggcgcggatg ctgcccgccg aggtcagggc gcgcatcggg
     4981 ccgttcacgc cgttgaggac gatggatccg gcgccggtct cgcagcgcac cccgcccgcg
     5041 gcgcggccga cccggatccc gcccccgccg gtggccgcgg tcacttcgcc ccccacgtcg
     5101 ccgatgtcga tgtctccgcc gttgctcgcc accttcacgg cggccgtggc gcgctcgatg
     5161 gcgacgctgc cgccgccggt ctccgcctcc accctgccgc cgacgcggcc gatgcggacg
     5221 tcccccccgg aggtgcgcac gaacaggtcc ccgtcccccg agtcgaccag cacgtcgccg
     5281 ccggcgctga tcagccgcgc cccgcccttc atgccggaga cggtgatggt gccggcgcgc
     5341 gtctcggcct gcagcggccc cggcagatcg cgcgcgacga tggcgccggc gcggctcaga
     5401 agcgtcacct tgccgggcgt cccctcggcg cggatgtcgc cggcgccggt ggtcaccgcc
     5461 acctctccga agctctccgg gatctcgatg tcgaaatcgg cgccgagtcc gcgggcgtcg
     5521 gccggcttcg gcagcgtgcc gatgaacgtg agcaggtccc cctcgcggct cgcgccgacg
     5581 accatgcgat cgagccggcg ccgcgcctcc gccatgtcgg gcccgagggc gcgcacccgc
     5641 acccggtacg tcacctcgcc ccccgggcgg cgcacgacac gcaccgcgcc gaggtgggtg
     5701 tcgaccttca agcgggccga ctcggccggg atggtgccgg cgatctcgtg caccatgttg
     5761 cgaccttcct ggtagtagct cacatggggg tcgcgcaccg gcgccgcggt caccgacagc
     5821 gcgaggcgga cggtgaacag ggccatgagc agcccggcga tcgccgtcat caggcgcgga
     5881 gcggcggaga cggtcgggtt cgggttcact ggcggttgct cgcgtacagg gcgtcgacga
     5941 aatcgccgga ccgcatccgg acgtaggcgt ccgggtcgtc gcgcgacagg cgctccat
//
LOCUS       DASRIQ010000164         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_837209, whole genome shotgun sequence.
ACCESSION   DASRIQ010000164 DASRIQ010000000
VERSION     DASRIQ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837209"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..733
                     /locus_tag="VFD06_03995"
     CDS             368..733
                     /locus_tag="VFD06_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02732.1"
                     /translation="MLRDKGTGSGLLLFVLAAVFAVAFGPGPAFGDRPPDGAAATTPV
                     LSIPEDPASTPVGSAEESLLPPLEGGFCPAVGPEDTDRFEVAPKPKFRTCRCSCGAPC
                     RTNEDCGPGGICSAGITCC"
     gene            complement(808..1668)
                     /locus_tag="VFD06_04000"
     CDS             complement(808..1668)
                     /locus_tag="VFD06_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02733.1"
                     /translation="MHQYSVRDVEKLAGLPRRRIRSLVEAGLVSPARGPRNTWLFSFQ
                     DLTVLRSARALATARVPRKRIRSSVEELKRRLPESAPLSGVSLSADADGVVIREGERR
                     WQAESGQYLLGFEEQGDAALAGVLPPGTPADATPRVLKRQPAARPESAEDWFERGAVL
                     EAQDQKSAREAYIQAIAADPGLLKARINLGCLLHEIGMLEQAETVYREALRVHGADPV
                     VLYDLGVLLEDMGRKREAMHAYETALRRDAGFADCHYNLALLCRGLGRPREAIQHMAA
                     YRRLTATTES"
     gene            complement(1690..>2026)
                     /locus_tag="VFD06_04005"
     CDS             complement(1690..>2026)
                     /locus_tag="VFD06_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZN02734.1"
                     /translation="EQQASDTFDPAPYKDEVRTRIEAAVQKKVEGQEITMTEAPEGGG
                     QVIDLMEALRASLERKAPGKARESQAAASETHAEARESRPAARETAARKPPQRAQDTE
                     TRARKTAKK"
BASE COUNT          279 a          702 c          721 g          324 t
ORIGIN      
        1 agcggtcgtg ccgaacccga cgacggtttc gccggaggcg gaaacgtcga aggcgccggg
       61 gcgaccatgc accggttgtc gttgctgtcc ggattcgatg tcgaggagga tcacccagag
      121 gccggcccgc ctgagtggca ttccggccag tataggaaac cggcgtggcc cgggcacccg
      181 cgttcttcag ccaaccggtc cgtgcgtgca gggaaccgat tgccgaaccg atcgcagacc
      241 tcccacgcca cgtcgaagtc gaggacgcgc tcgattgcga cgtgagcgtt ccgggcgtaa
      301 aatgcgcggc atcgacagcc acgaatcgcc acagggccgg gtccgtccaa tcgaaggagg
      361 agcgctcatg cttcgagaca agggaacggg aagcgggctg ttgctgttcg tccttgctgc
      421 agtcttcgcg gtcgcattcg gcccggggcc ggccttcggc gaccggccgc cggacggggc
      481 ggcggccacc acgccggtcc tttcgatccc ggaggatccg gccagcacac cggtcggctc
      541 cgccgaggaa agcctgctcc ccccgctgga gggcggattc tgcccggcgg tcgggccgga
      601 agacacggac cgattcgagg tggcgccgaa gccgaagttc cgcacctgcc gctgcagctg
      661 cggagccccg tgccggacca acgaggactg cggaccggga gggatctgct ccgccggcat
      721 cacctgctgc tgagacattc gcgagtcgtg ctaggtccgc cggcgccgct caaagagccg
      781 gtggcgccgg cggatacctg tatgtgtcta cgactcggtc gtcgcggtca ggcgacggta
      841 ggcggccatg tgctggatcg cctcgcgggg acggcccagc ccccggcaga gcagggcgag
      901 gttgtagtga cagtcggcga accccgcatc cctgcgcaag gcggtctcgt aagcgtgcat
      961 cgcttcccgc ttccggccca tgtcctcgag caggacgccg aggtcgtaga gcacgaccgg
     1021 gtcggctccg tgcacacgca gggcctcgcg gtagaccgtc tcggcctgct cgagcatccc
     1081 gatctcgtgg agcaggcatc cgagattgat ccgggccttg aggaggccgg gatcggcggc
     1141 gatcgcctgt atgtaggcct cgcgcgcgga tttctgatcc tgcgcctcga ggacggcgcc
     1201 gcgctcgaac cagtcttcgg cgctctcggg ccgcgccgcg ggttgccgct tgagcaccct
     1261 cggagtcgcg tcggccgggg tcccgggcgg caggacaccg gcaagggctg cgtcgccctg
     1321 ctcttcgaag ccgagcaagt actgccccga ttcggcctgc cagcggcgtt ccccttccct
     1381 gatcacgacg ccgtcggcgt ccgccgaaag gctcactccg gacaggggcg ctgactcagg
     1441 aagacgacgc ttcagctcct cgacggagga gcggatgcgc ttgcgcggca cgcgggccgt
     1501 cgcgagcgcc cgggcgctgc gcaggaccgt caggtcctgg aaggagaaca gccaggtgtt
     1561 ccgcggcccg cgcgccgggg acacgagccc ggcctcgacg agggagcgga tccggcggcg
     1621 cgggagaccg gcgagcttct cgacgtcgcg cacgctgtac tggtgcatgg cgggccggtc
     1681 cctccactct tatttcttgg cggtcttcct ggctcgggtt tccgtgtcct gggcgcgctg
     1741 cggcggcttg cgggccgccg tctcccgcgc cgccggccgg gactcgcgcg cctcggcgtg
     1801 ggtctcgctc gccgccgcct gggattcgcg cgccttgccg ggcgccttcc gctccaggct
     1861 ggcgcgcagc gcctccatga gatcgatgac ctggccgcca ccctcgggcg cctcggtcat
     1921 ggtgatctcc tggccttcga ccttcttctg caccgccgcc tcgatgcgcg tgcgcacttc
     1981 gtccttgtac ggagcgggat cgaaggtgtc ggaggcctgc tgctcg
//
LOCUS       DASRIQ010000165         4198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_591860, whole genome shotgun sequence.
ACCESSION   DASRIQ010000165 DASRIQ010000000
VERSION     DASRIQ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591860"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFD06_04010"
     CDS             complement(<1..495)
                     /locus_tag="VFD06_04010"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZN02735.1"
                     /translation="MDILDRILAAKRVELEAEKRSIPPRRAIEMASSAAPPHAFLPAL
                     KRSGRVNVIAEVKRASPAKGAIRPGAAPSEVAKAYAEAGAAAISVLTDARFFLGAPEH
                     LQEVRRQVDVPLLRKDFLFEEYQIYRSRALGADAVLLIARVLDPRLLNTLIGITRSLD
                     MEALV"
     gene            complement(508..1542)
                     /gene="trpD"
                     /locus_tag="VFD06_04015"
     CDS             complement(508..1542)
                     /gene="trpD"
                     /locus_tag="VFD06_04015"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZN02736.1"
                     /translation="MSSPDPFLAILRKAVAGERLAAEEAGAAFDAVMEGTVSPARLAA
                     LLVALRMRGETVEEIAAFARAMRARAVPVTSRHADLLDTCGTGGDGAGTFNVSTVAAL
                     VAAAAGVPVAKHGNRAVSGRCGSADLLEGLGVRADLAADEAAACLDDLGFGFLFAPRL
                     HPSMGHAAGVRRELGVRTVFNLLGPLANPAGARRQVLGVYDPAWVEPLARVLVELGTT
                     RALVVHGAGTDEIALHDATIAAEVRDGRVTLLTLQPEEAGLRRAPLQAIAGGDPPAQV
                     EAARRVLEGERGPRRDVVVLNAGAALWVADRTAGLEEGARLAEETLDRGLASELLERL
                     RRRTPHRGST"
     gene            complement(1539..2114)
                     /locus_tag="VFD06_04020"
     CDS             complement(1539..2114)
                     /locus_tag="VFD06_04020"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZN02737.1"
                     /translation="MILMIDNYDSFTWNLVQYLQEMEQEVEVVRNDAIDADGIAALGP
                     KAIVISPGPGTPDDAGVSLETVRRFSGRLPILGVCLGHQAIGQAFGGRIVRAPTLMHG
                     KTSPIRHDDGPLYHGLPNPFEATRYHSLVVDPKSFPQALVVSATTDAGVIMGLRHRSH
                     PTEGVQFHPESILTTVGKDLMRNFLRAAGIA"
     gene            complement(2111..3595)
                     /locus_tag="VFD06_04025"
     CDS             complement(2111..3595)
                     /locus_tag="VFD06_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZN02738.1"
                     /translation="MTRGEFNFGPGDLARLARPGHVVPLCREIGADLITPVGAAMRLR
                     RGARAPFLLESIEGGETVARYSFLGRDPVGWLRLLDDEPDAGHGRAARPLGALRQALR
                     PFRPVRLPDLPRFVGGAVGYASYDLARRFERLPRSAHDDLCVPALACGLYDTVVAFDH
                     LRQRLQIVTLVAAEHGGRPLRGAALGRSWEAGRRRIEAIERALARPLRAASRPRRRPG
                     RWRASTSRSDYLAAVRRARAAIARGDIFQVVLSQRFDRPAVAPPLGVYRALRRINPSP
                     YMFHLDHDGAALSGASPEMLVRVDGGRVRTRPIAGTRPRGRDEEEDRRLERALRADAK
                     ERAEHVMLVDLARNDLGRVCRAGSVVVSRFLQVERFSHVMHLVSEVEGLLRPGCDALD
                     AFAAAFPAGTVSGAPKVRAMQIIDALEPVRRGPYAGAVFWADGAGALDSCLTIRTVLE
                     RNGTVHVQAGAGIVADSRPAREHRECLAKAQALIAAVERAEAAP"
     gene            complement(3592..>4198)
                     /locus_tag="VFD06_04030"
     CDS             complement(3592..>4198)
                     /locus_tag="VFD06_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02739.1"
                     /translation="GACVGLRRNPNVPGTYSDKLSPYNYREDGSLASMVVGVDAARFI
                     RKEPFVPLFVQVINKSKGTFEVTRESFLLEDRLGKQYGVAPAAEVADAYPRLDIDRRI
                     FTQNRSITLTYVSLYTYITSDFFPSSARRSLLVDHVSMPPRSYMEDVLYFPVPESGLN
                     DVPLRLLFQVPGLADPIQVVFEVPLTPGILEKELKEKQGGS"
BASE COUNT          594 a         1566 c         1513 g          525 t
ORIGIN      
        1 gacgagcgcc tccatatcga gcgatcgggt gatcccgatg agggtattca ggagccgcgg
       61 atcgagaacg cgcgcgatca ggagcaccgc gtcggcaccg agcgcgcggc tgcggtagat
      121 ctggtactcc tcgaagagga agtccttcct caggagcggc acgtccacct gccggcgcac
      181 ctcctgcagg tgctccgggg cgccgaggaa gaaccgggcg tccgtcagga ccgagatcgc
      241 cgccgcaccg gcctcggcgt acgccttcgc gacctcgctc ggagccgccc cggggcggat
      301 tgcccccttc gcgggagagg cgcgcttcac ctcggcgatg acattgacgc ggccggaacg
      361 cttcagggcc gggaggaagg cgtgcggcgg ggcggccgag ctcgccatct cgatggcccg
      421 gcgcggcggg atgctccgtt tctccgcttc gagctccacc cgcttcgccg ccaggatccg
      481 gtccaggatg tccatgcgcc tcccgtccta cgtcgaacca cgatgcggcg tacgccgccg
      541 caggcgctcg aggagctctg aggcgaggcc gcggtcgagg gtctcttccg cgagccgcgc
      601 cccctcctcg aggccggccg tccgatccgc cacccacagc gcggcaccgg cgttcagcac
      661 caccacgtcc cgccgcgggc cgcgctcccc ttcgaggacg cgccgcgccg cctccacctg
      721 cgccggcgga tcaccgccgg cgatcgcctg gagcggcgcc cgtcggagcc ccgcctcctc
      781 cggctggagc gtgagcaggg tcacgcggcc gtcccgcacc tccgccgcga tcgtcgcgtc
      841 gtgcagggcg atctcgtcgg tccccgcgcc gtgcacgacc agcgcccgcg tcgtccccag
      901 ctcgaccagg acccgtgcca gcggctccac ccaggcggga tcatagacgc ccaggacctg
      961 ccggcgcgcg ccggccgggt tcgcgagcgg gcccaggagg ttgaaaacgg tccgtacccc
     1021 gagctcccgc ctcacgccgg cggcgtgccc catcgaaggg tgcagccgcg gcgcgaacag
     1081 gaacccgaag ccgagatcgt cgaggcaggc ggccgcctca tcggccgcga ggtcggcccg
     1141 gaccccgagc ccttcgagca gatcggcgct gccgcagcgc ccgctcacgg cgcggttgcc
     1201 gtgcttggcc accggcacac ccgccgccgc ggccacgagc gccgcgacgg tcgagacgtt
     1261 gaaggtgccg gcgccgtcgc cgccggtgcc gcaggtgtcc agcaggtcgg catggcggct
     1321 cgtcaccggg acggcgcggg cccgcatggc ccgcgcgaag gcggcgatct cctccaccgt
     1381 ctcgccgcgc atgcgcagcg ccacgagcag cgccgccagc cgcgccggcg agaccgtccc
     1441 ctccatgacc gcgtcgaagg cggcccccgc ctcctccgcg gcgaggcgct ccccggcgac
     1501 cgccttccgg aggatcgcga ggaacgggtc gggggagctc atgcgatccc ggccgcgcgc
     1561 aggaaattgc gcatcaggtc cttccccacc gtcgtgagga tcgactcggg gtggaactgg
     1621 accccctcgg tcgggtggga gcggtgccgc agtcccatga tcacacctgc gtcggtcgtg
     1681 gcggagacca cgagcgcctg ggggaagctc ttcggatcga ccacgagcga atggtagcgg
     1741 gtcgcttcga acgggttcgg cagcccgtga tacagcggac cgtcgtcgtg ccggatcggg
     1801 ctcgtcttgc cgtgcatgag ggtcggcgcg cggacgatcc ggcctccgaa cgcctgcccg
     1861 atcgcctggt gcccgagaca gacgccgagg atcggcaggc gtcccgagaa ccgccgcacg
     1921 gtctcgaggc tcaccccggc gtcgtccggc gtgccgggcc cgggggagat gacgatcgcc
     1981 ttcgggccga gcgcggcgat cccgtccgca tcgatcgcgt cgttgcggac gacctcgacc
     2041 tcctgctcca tctcctggag gtactgcacc aggttccagg tgaaggagtc gtagttgtcg
     2101 atcatcagga tcacggcgcc gcctccgccc gctcgaccgc ggcgatcagc gcctgcgcct
     2161 tggccaggca ctcccggtgc tcgcgcgccg ggcgcgagtc ggcgacgatc ccggcgccgg
     2221 cctggacgtg caccgtcccg ttgcgttcca ggacggtgcg gatggtgagg caggagtcga
     2281 gcgccccggc cccgtccgcc cagaagacgg cgccggcgta cggcccgcgg cgcaccggct
     2341 ccagggcgtc gatgatctgc atggcgcgca ccttgggcgc tcccgacacg gtcccggccg
     2401 gaaaggcggc cgcgaaggcg tccagggcgt cgcaccccgg gcgcaggagc ccttccacct
     2461 ccgacacgag gtgcatgacg tgcgagaacc gctcgacctg gaggaagcgg ctcaccacga
     2521 cgctgccggc gcggcagacg cgcccgaggt cgttgcgcgc caggtcgacg agcatgacgt
     2581 gctccgcccg ttccttggcg tcggcgcgca gcgcccgctc caggcgacgg tcctcctcct
     2641 cgtcgcggcc gcgcggccgc gtcccggcga tcggccgcgt gcgcacccgc ccgccgtcga
     2701 cccgcacgag catctccggc gaggcgcccg acagggccgc cccgtcgtgg tcgaggtgga
     2761 acatataagg ggacggattg atgcgccgga gcgcccggta gacgccgagc ggcggcgcca
     2821 ccgcgggccg gtcgaagcgc tgcgacagga ccacctggaa gatgtcgccg cgcgcgatcg
     2881 ccgcgcgcgc gcgacgcacc gccgccaggt agtcggaacg gctcgtcgag gccctccagc
     2941 gccccgggcg gcggcgcggt cgggacgcgg cgcggagcgg ccgcgcgagc gcccgctcga
     3001 tcgcctcgat gcgccgccgc cccgcctccc aggagcgacc gagcgcggcg ccgcgcagcg
     3061 gccggccacc gtgctccgcg gcgacgagcg tgacgatctg cagcctctgg cgcaggtggt
     3121 cgaaagcgac gacggtgtcg tacagcccgc aggcgagggc cgggacgcag agatcgtcgt
     3181 gcgccgaccg gggcagccgc tcgaagcgcc gcgccaggtc gtacgaggcg tagccgaccg
     3241 cgcccccgac gaagcgcggc agatccggca gccggaccgg ccggaaggga cgaagcgcct
     3301 gccgcagcgc cccgagcggc cgcgccgccc gtccgtggcc cgcgtccggc tcgtcgtcca
     3361 agagccgcag ccacccgacc gggtcgcgcc ccaggaacga gtagcgcgcc acggtctccc
     3421 ctccttcgat cgactcgagc aggaacgggg cgcgcgcccc gcgccgcagg cgcatcgccg
     3481 cccccaccgg ggtgatcaga tcggcgccga tctcgcggca cagcggcacg acgtgacccg
     3541 ggcgcgcgag gcgggccaga tcgcccggac cgaagttgaa ctccccccgg gtcacgatcc
     3601 gccctgcttc tccttcagtt ccttctcgag gatgccgggc gtcaggggca cttcgaacac
     3661 cacctggatc gggtcggcga gccccggcac ctggaacagc agccgcagcg ggacgtcgtt
     3721 caacccgctc tccgggaccg ggaagtacag cacgtcctcc atgtaggagc gcggcggcat
     3781 cgacacatgg tcgacgagca gcgaccggcg cgccgaggag gggaagaagt cgctggtgat
     3841 gtaggtgtac aggctgacgt aggtcagcgt gatcgagcgg ttctgcgtga agatgcgccg
     3901 gtcgatgtcg aggcgcggat aggcgtcggc cacctcggcg gcgggggcga cgccgtattg
     3961 cttgccgagg cgatcctcga ggaggaacga ctcgcgggtg acctcgaatg tccctttcga
     4021 cttgttgatc acctggacga agagggggac gaacggctcc ttgcggatga agcgggcggc
     4081 gtcgacgccg accaccatcg aagcgaggct gccgtcctcg cggtagttgt acgggctgag
     4141 cttgtcgctg taagtccccg ggacgttggg gttccgcctc aggcccacgc aggcgccg
//
LOCUS       DASRIQ010000166         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1370037, whole genome shotgun sequence.
ACCESSION   DASRIQ010000166 DASRIQ010000000
VERSION     DASRIQ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370037"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1943
                     /locus_tag="VFD06_04035"
     CDS             <1..1943
                     /locus_tag="VFD06_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02740.1"
                     /translation="GQRAFAGDSAVETMHAILKNDPKDLAAIDPGLPAALDRIVRHCL
                     EKNPEERYQTARDVAFSLEAFAGGSTATSGPQASAPLARAMGRRRLVAGLGILGAAAC
                     LAGGVLLGARLGDRRPSGPPSFTRLTFRHGFIHSARFVNQGSGIVFGAAWDGQPLDIY
                     LAKPGVPEARSLGLQGLNVLAVSRSDELAVCRVTRRGFTNLYRSTGTLGRMPLNGGAP
                     RDIVENVMYADWAPDGASLAVIRDLGNHRRLEYPVGTLLYETQHNLFNPRVSPDGKRV
                     ALWETSARSARVIVIDAQGTVRTLSPDWADWWYLAWSPDGREIWYGAAKSVGFSSTVY
                     AVDLEGHRRTLYAGPGDYDVHDVLADGRALLGSVKQRLRTVGLLDGWGRTRDLSWFDG
                     TMVIDIAADGTSLLLSEESEAGGPDESVYLQRSDGAPPVRLGEGRALALSPDGRLALA
                     ARSRREIVIMPIGAGVEKPLASGVFESVGEARWLPDGRRFAVQASLPGQRTQLFLLDV
                     GGGTPLALADADYGDTSLIAVSPDGARIAAVTVDERIALVPVAGGTPVFDDAFRPGDQ
                     PIAWSADSAALYVYRREAPTATVERVDLRTRRREIWKELPVTDSAGVSSIQRVVMTSD
                     AKSYAWAWQQLLTDLYLVEGLR"
     gene            2001..2393
                     /locus_tag="VFD06_04040"
     CDS             2001..2393
                     /locus_tag="VFD06_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02741.1"
                     /translation="MSTTRIAVAALAVLSILAVARHEPVPAQAAGKSCKVSLTVGLNN
                     TTPNDVKTKYTFGIGANSAEACADVVYGLVITEQLPDGSRKTSEIPGDMRVRGQTRIE
                     VVTYDTDSKNTLKGFEAKMKSCQVCDAP"
     gene            complement(2390..>2838)
                     /locus_tag="VFD06_04045"
     CDS             complement(2390..>2838)
                     /locus_tag="VFD06_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02742.1"
                     /translation="TASLQTILPATDATRHQVPYVEHAHVLDFLGGQIGWVKGWTHHL
                     GDFLFYTLDGGRTWKRADVEQRPYDRLLRLGDGSWLGLVLPHEIRILKDGVFVPLRVF
                     PRAIDWTIVDATGSLVLHLDGGETWQLQGDGKDWLLLSRRDSANEN"
BASE COUNT          439 a          979 c          989 g          431 t
ORIGIN      
        1 cggggcagcg ggccttcgcc ggcgactcgg cggtcgagac gatgcacgcg atcctcaaga
       61 acgacccgaa ggatctggcg gcgatcgacc cggggcttcc ggcagcgctc gatcggatcg
      121 tccggcactg cctcgagaag aatcccgagg agcgctacca gaccgcgcgc gatgtggcct
      181 tctcgctcga ggcgttcgcg ggcggatcga cggcgaccag cgggccccag gcttccgcgc
      241 cgctggcgcg cgccatgggt cgccggcggc tcgtcgccgg cctggggatc ctgggtgccg
      301 ccgcctgcct ggccggggga gtcctcctcg gtgcccggct cggcgaccgg cgcccctccg
      361 gcccgccgtc gttcacccgt ctgaccttcc ggcacggctt catccactcg gcgcgctttg
      421 tcaaccaggg gagcggcatc gtcttcggcg ccgcctggga cgggcagccg ctggacatct
      481 acctggcgaa gccgggggtg ccggaggcgc gttctctcgg cttgcagggc ctgaatgtcc
      541 tcgcggtctc gcgctccgac gagctggccg tgtgccgcgt cacccgacgc ggctttacaa
      601 acctctaccg gtccaccgga acgctgggcc ggatgccgct caacggcggg gcgccgcgcg
      661 acatcgtcga gaacgtcatg tacgcggact gggctcccga cggcgccagt ctggccgtga
      721 tccgggatct cggcaaccat cgccggctcg agtacccggt cgggacgctt ctctacgaga
      781 cccagcacaa tctcttcaat ccgcgggtct cccccgatgg gaaacgggtc gcgctctggg
      841 agacgtcggc gcgaagcgcc agggtgattg tcatcgatgc gcaggggacg gtacgcacgc
      901 tcagcccgga ctgggccgac tggtggtacc tcgcatggtc ccccgacggg cgggagatct
      961 ggtatggagc ggcgaagagc gtcggcttca gctccacggt ctacgccgtc gacctggaag
     1021 gacaccggcg caccctttat gccggcccgg gtgactacga tgtccatgac gtcctggcgg
     1081 acgggcgcgc gctcctcggc tcggtcaagc agcgcctccg gacggtgggc ctgctcgacg
     1141 gctggggccg gactcgcgac ctgtcgtggt tcgacgggac catggtgatc gacatcgccg
     1201 cggacgggac atcgctgctc ctgtccgagg agagcgaggc gggcggaccc gacgagtcgg
     1261 tctacctcca gagaagcgac ggcgcaccac cggtccgcct gggggagggc cgggcgctcg
     1321 cgctctctcc ggacggccgc ctggcgctcg cggcccgctc ccggagggag atcgtcatca
     1381 tgccgatcgg cgccggggtc gagaagccgc tggcctccgg agtcttcgaa tcggtggggg
     1441 aagctcgctg gctgccggac ggaaggcggt tcgccgtcca ggccagcctg ccgggacagc
     1501 gcacgcagct gttcctcctc gatgtcggcg gcggcacgcc gctggcgctg gccgatgcgg
     1561 attacggcga taccagtctc atcgccgtgt cacctgatgg ggcccggatc gccgccgtca
     1621 ccgtggacga acggatcgcg ctggtgccgg tggcgggggg aacgccggtc ttcgacgacg
     1681 cgttccgccc cggcgaccag ccgatcgcct ggagcgcgga cagcgccgcc ctctacgtct
     1741 accggcgcga agccccgacc gccacggtcg agcgggtgga cctgcgcacg cgccgtcgcg
     1801 agatctggaa ggagctgccg gtcaccgatt ctgccggggt gtccagcatc caacgggtgg
     1861 tcatgacctc cgacgcgaag tcctatgcct gggcctggca gcagctcctc acggatctgt
     1921 atctcgtcga agggttgcgg tgatccgcgg cggaccctat acatagccgg gagcacgtgt
     1981 caccgagggg agaccacgcc atgtccacca cccgaatcgc cgtcgccgcg ctcgcggtcc
     2041 tgtcgatcct ggccgtcgcc cggcatgagc cggtccctgc gcaggccgcg gggaagtcgt
     2101 gcaaggtcag cctgaccgtc ggtctcaaca acaccacgcc gaacgacgtg aagacgaagt
     2161 acacgttcgg catcggggcg aacagcgccg aggcgtgcgc cgacgtggtc tacgggctcg
     2221 tgatcaccga gcagctcccc gacggcagcc gcaagaccag cgagatcccg ggggacatgc
     2281 gggtccgggg tcagacgcgc atcgaggtcg tcacctacga caccgactcg aagaacacat
     2341 tgaagggctt cgaggcgaag atgaagtcct gccaggtctg cgacgccccc tagttctcgt
     2401 tcgcggagtc gcggcgggag agcaacagcc aatccttccc gtccccctgc agctgccagg
     2461 tctcccctcc gtcgagatgc aggacgaggc tgccggtcgc gtccacgatc gtccaatcga
     2521 tcgcccgcgg gaaaaccctc agggggacga agaccccgtc cttgaggatg cgaatctcgt
     2581 gcggcaggac gagccccagc caggaaccgt cgccgagtcg aagcaggcga tcgtagggcc
     2641 gttgctccac gtccgccctc ttccacgtcc tgccgccatc gagggtatag aacaggaagt
     2701 cacccagatg gtgggtccaa cctttgaccc agcctatctg cccgccgaga aagtcgagga
     2761 cgtgcgcatg ctcgacgtac gggacctggt gccgggtcgc atcggtggcg ggaaggatcg
     2821 tctgaagtga cgcggtcg
//
LOCUS       DASRIQ010000167         7726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1371010, whole genome shotgun sequence.
ACCESSION   DASRIQ010000167 DASRIQ010000000
VERSION     DASRIQ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371010"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFD06_04050"
     CDS             complement(<1..757)
                     /locus_tag="VFD06_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02743.1"
                     /translation="MKHDSVISPLPGWARLVRPLALLALAFGFASSEARAQAISFGKS
                     VLQGTSLTSPTALQFGPDNRLYVTQQNGTILIYTLTRNGPNSYVVTATQTITSVRDIP
                     NRNDNGALNASVTGRQVTGILVTGTAANPVIYVASSDPRIGAGSGGTDSNLDTNSGIL
                     SRLTWNGSAWVKLDLVRGLPRSEENHASNGLALIDSGNTLLVAQGGNTNMGAPSNNFA
                     LLPEYALSAAILSIDLAAIGSTTYDLLTLDDPAP"
     gene            831..2435
                     /locus_tag="VFD06_04055"
     CDS             831..2435
                     /locus_tag="VFD06_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN02744.1"
                     /translation="MDRNEGARRGGGDHRSGIALLLGLSFAALAAPLSAFATRAAAPA
                     PASPAAPADAASKRPRLVLLVVIDQFRADYLDRFLPRFGPDGFKRLLGDGTLFTGCAY
                     SYAFTETAPGHATIATGTTPDRHGVVANEWWDAALGRTTEAIEDPEAPLFGPPGTTGA
                     SPRRLLVETIGDVLRRETKGAAKVWAVAGKDRSAIFSAGLRPSGAVWYDKRNGRMVTS
                     RYYATEPPEWLRRFNGARPAERLLRNEQVKDFSSLRTTPQFHDLLLRCARTIVASEQL
                     GADDVPDLLMVGLSATDLLGHEVGPFDDRLADLVVRLDGQLAGFLKFLDERVGPGRTI
                     VALSSDHGVAPTRAQAIASGLTPPALDRRRLRETLERALAAAILGEKPAPRLHGASST
                     RLWFDDDELARAGMTKEQAEEIVGQAAAGIEGIAGWVTPRRATVEASMAELYRLSRYA
                     GRSPDLCLVPAEFAIDATGDPATHGSPWPYDRRVPLLFAGAPFRHGARDEQPCSPADL
                     APTLAAALRIPPPAGATGRVLSSALP"
     gene            2578..4833
                     /locus_tag="VFD06_04060"
     CDS             2578..4833
                     /locus_tag="VFD06_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN02745.1"
                     /translation="MSLPAVLVGTLTLGLSGIGLPATALAVPTLAPGFSMTTVVGGMG
                     DPLSFDWAPNGDLFIAEKSGKVRLFRSGVLSIIGTIPVSVTDELGITAIEVDPEFATN
                     HYLWMTYSTPSPVRQRVARFTIVNDQLTAETVVVEYPVTFPLHHAGCIRFAADGTLFI
                     STGDDGLLSTGSQNPFDLRGKILHINRDGTPAAGNPFASGAQGDPRVWALGFRHPWRF
                     TIQPETGTLFIADIGAAEWEELDLGIPGGNYGWSTVEGPAPPGQAGFVYPIDSYPHTN
                     PAGASIMAGPFATDADFNGEFTGQFFFADEAARDLRRMVLDASNNVILTEIWATDLQN
                     PVEIRFGPDGAFYFVSFNAGAVYRIARVGGANRQPQAIGAAVPASGPAPLQVLLDGTA
                     SSDPDQQQTLQFAWTAGHQGATGTQATLNHLYPAGVYFARLTVNDGQGGTDATPDIRI
                     VSGNRAPTATITAPLDEAHYQAGDTIDFSGGGSDPEEGTVPCSRFTWSVTFHHAGHTH
                     PHLGPIEGVCGGSFVIPRTGETSADVFYEILLVVNDTGVPLGAAAVLTGATAVQVRPD
                     TAPLTFATAPHPGLLVLLDGTLQPAPIVTDAVVNFFRSIGAPASQTGPDGRTYTFASW
                     SDGGLQSHTIVVPAIPAQYTATFTCGVINEVAGLQLAHATGGQVALSWQPISDLCAAT
                     TGVRYLIYASTNARPSVLPGSFPTDPAFVLRGMVTGTSFTYLPDPSDTFFLVVAVGSD
                     GSAGRAGHYGG"
     gene            5008..6318
                     /locus_tag="VFD06_04065"
     CDS             5008..6318
                     /locus_tag="VFD06_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HZN02746.1"
                     /translation="MRLAALSLPGLLLVGAAVLAAIPFAAPAEAPSSPSTGRPLLVTI
                     DDLPVASPKLHTDPADREEVTRKLLEILKAHKVPAAVFTIWGQVKGPKDTALLDRYRA
                     AGHELGNHSFGHLDLPRTPVAAYVEDIERGRAGLAGYLAPHRLEVRFFRFPYLREGET
                     GEQWDAVRKALRASKQRVMPVTIDTQDWSFEEPYVAAVRRGDATAARDVAADCLAALK
                     LEVRRHEERGDDLFGRQTPQILLLHANAVGTAVWDDLFSWLKRTGHRFATADEVLADP
                     AFSEEPRFLARYGCSLWDRIGHEREAKESAAAIRKLIADQVEAWNRGDLVAFTNVYDD
                     DATFLSPTGSVRGRQAVHERYTKRYPDRAAMGTLKLEIDDIRPVWGMEITPGGDAVPG
                     RIHGASASGRWTLSYPDREAATGRTLLVLSRAGTRWTIVQDASM"
     gene            6315..>7726
                     /locus_tag="VFD06_04070"
     CDS             6315..>7726
                     /locus_tag="VFD06_04070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN02747.1"
                     /translation="MSGPPGDPFAIGGKRGRLFLGILLAGAALRLAYVARPLDHRMRA
                     PWRQADYLMIARNFAREDGCILHPRIDWRGDTPGLVEMEAPILPWIGGMLFRVFGVHA
                     GILRALSCLAELLALLLFAGLARRMLPDAGATLALAAFALNPLLVDLATALQPEPLML
                     LLCIAVVVCLERYDAVPPTEGRAVGREEWLLLCGAALGGAILAKAPAACLGILIGWVV
                     LRRMGTAALLRPAVWGTALLALVPPALWYAWAKRYYLLYGNSMGVSNEYPFLGFDMLW
                     PPRFFLGLFKWETLGVVTPIGWILMIASLSAPWKVLERPLVWYLSVCVFLVVGARTTA
                     DDWAFYYHSLAVAPACLLMGMGFVAFSQAWPGAARAAAAATCLCLAGATGYILYARDH
                     RPDLEAMRRCGLQFVAHIPEGATVVTRGGTLRDEEGTPVAHNESMLFAWLDRRGFTYG
                     NEELSFPLLDRIAARGGRFW"
BASE COUNT         1048 a         2769 c         2709 g         1200 t
ORIGIN      
        1 acggcgccgg gtcgtcgagg gtcagcaggt cgtaggtcgt cgagccgatc gcggccagat
       61 cgatggacag gatggcggcc gacagcgcgt actccggcag gagcgcgaag ttgttcgacg
      121 gcgcccccat gttggtgttg cccccctgtg cgaccagaag cgtgttgccg gagtcgatca
      181 gcgccagtcc gttcgaggcg tggttctcct cggagcgcgg caggccgcgc acgaggtcga
      241 gcttcaccca cgccgagccg ttccaggtca ggcgcgacag gatgcccgaa ttggtgtcga
      301 ggttgctgtc ggtcccgccc gatccggcgc cgatgcgcgg gtcgctcgag gcgacgtaga
      361 tcaccgggtt ggcggcggtg ccggtgacga ggatcccggt cacctggcgc ccggtcaccg
      421 aggcgttgag cgcgccattg tcgttgcggt tcgggatgtc gcggaccgag gtgatggtct
      481 gggtcgcggt gacgacgtac gagttcggcc cgttccgcgt cagggtgtag atgaggatcg
      541 tgccgttctg ctgcgtgacg tagagccggt tgtcgggccc gaactgcagc gccgtcgggc
      601 tcgtcagcga ggtcccctgc aggacgctct tgccgaagga gatcgcctgc gcccgcgcct
      661 cggatgaagc gaagccgaag gcgagcgcga gaagcgccag cgggcgcacc aggcgcgccc
      721 atcccgggag gggggagatg acggaatcgt gtttcatctg gagagtgcga ccaccttcat
      781 gtacggtgat ccctccccgc cgtcaaggcg cactgtcata tgctgcgccg atggatcgga
      841 acgaaggggc gaggcgcggc ggaggcgatc accgttccgg gatcgccctg ctgctgggct
      901 tgtctttcgc ggcgctcgcc gcgccgctgt ccgccttcgc gacgcgcgcg gcggccccgg
      961 cgccggcgag ccccgcggcg ccggcggacg ctgcatcgaa gaggccgcgc ctcgtcctcc
     1021 tcgtcgtcat cgatcagttc cgggccgact acctcgaccg gttcctcccg cgcttcggtc
     1081 cggacggctt caagcgcctc ctcggggacg ggaccctgtt caccggatgc gcttactcgt
     1141 acgccttcac cgagaccgcc ccggggcacg ccacgatcgc gaccggcacc actccggatc
     1201 ggcacggcgt ggtcgccaac gagtggtggg acgccgcgct ggggcggacg accgaggcga
     1261 tcgaagatcc cgaggctccc ctgttcggcc cgccggggac gaccggggcg tcgccgcgga
     1321 ggctcctggt cgaaacgatc ggcgacgtgc tgcgccggga gacgaagggg gcggcgaagg
     1381 tgtgggcggt cgccggcaag gatcgctcgg cgatcttctc ggccgggctc cgtccctcgg
     1441 gggcggtctg gtacgacaag cgcaacgggc gcatggtcac gagccgctac tacgccaccg
     1501 agccgccgga gtggctgcgg cgcttcaacg gcgcccggcc cgcggagcgc ctgctgcgca
     1561 acgagcaggt gaaggatttc tcgtcgctgc ggacgacgcc ccagttccac gatctcctgc
     1621 tccggtgcgc ccgcaccatc gtggcgtccg agcagctcgg ggccgacgac gtccccgatc
     1681 tcctgatggt cggactctcg gcgaccgacc tcctggggca cgaggtcgga ccgttcgacg
     1741 atcggctcgc cgacctcgtc gtccggctcg acggtcagct cgccgggttc ctgaagttcc
     1801 tcgacgaacg ggtcggcccg gggcggacga tcgtggcgtt gtcgagcgat cacggcgtag
     1861 cgccgacccg cgcccaggcg atcgcctccg gcctgacgcc gccggcgctc gaccgccggc
     1921 gcctccgcga gacgctggag cgggccctcg ccgcggcgat cctgggagaa aagccggcgc
     1981 cgcggctcca cggagcctcc tccacccgtc tctggttcga cgacgacgag ctggcgcgcg
     2041 ccggcatgac gaaagagcag gcggaagaga tcgtgggaca ggcggcggcc ggaatcgaag
     2101 ggatcgccgg atgggtcacg cctcgccgag ccacggtcga agcctcgatg gcggagctgt
     2161 accgcctgag ccgctatgcg gggcgcagcc ccgatctctg tctcgtgccg gccgagttcg
     2221 cgatcgacgc cacaggggat cccgccacgc acggctcccc ctggccctat gaccgccgcg
     2281 tgccgctctt gttcgccgga gccccttttc ggcatggggc gcgcgacgaa cagccctgct
     2341 cgcctgccga cctcgctccg acgctggcag cggcgctgcg gatcccccct cccgccggag
     2401 ccaccggccg cgtcctgagc tccgctcttc cctaggagcc tggcctccgc tctcccctag
     2461 cgacagcaac acgggatcct gcctggctta cggacgattg cgaggtggct ctgagcctct
     2521 cctcgccgta cactcccggc ccgcatgccc tcccaggcgc acaggcgcac gcaacgagtg
     2581 tccctcccgg ccgtcctcgt cggaacgctg acgctcggac tgagcgggat cgggctgccg
     2641 gccaccgcgc tggcggtccc gaccctcgcc cccggcttct ccatgaccac ggtcgtcggc
     2701 ggcatggggg atccgctttc cttcgactgg gcccccaacg gcgatctctt catcgccgag
     2761 aagtcgggga aggtgcggct cttccggagc ggggtcctgt cgatcatcgg aacgatcccg
     2821 gtgagcgtga ccgacgagct ggggatcacc gccatcgagg tggatccgga gttcgcgacc
     2881 aaccactacc tctggatgac ctacagcacg ccttcgccgg tgcggcagcg ggtcgcccgc
     2941 ttcaccatcg tcaacgatca gctgaccgcc gagacggtcg tcgtcgaata cccggtcacc
     3001 ttccccctgc accacgccgg ctgcatccgc ttcgcggcgg acgggacgct cttcatcagc
     3061 accggcgacg acgggctgct gtccaccggg tcacagaacc cgttcgacct gcgcggcaag
     3121 atcctgcaca tcaaccggga cggcacgccg gccgccggca atccgttcgc aagcggcgcg
     3181 cagggggatc cgcgcgtctg ggcgctcggg ttccggcacc catggcgctt cacgattcag
     3241 ccggagaccg gaaccctctt catcgccgac atcggcgcag cagagtggga ggagctcgac
     3301 ctcgggatcc cgggagggaa ctacggctgg tccacggtcg aggggccggc gccgccgggg
     3361 caggcggggt tcgtctaccc gatcgactcg tacccgcaca cgaaccccgc cggcgcctcg
     3421 atcatggccg gtccgttcgc gaccgacgcc gacttcaacg gagagttcac cgggcagttc
     3481 ttcttcgcgg acgaggcggc ccgcgacctg cgacgcatgg tgctcgacgc ctcgaacaac
     3541 gtgatcctca ccgagatctg ggcgaccgac ctgcagaacc cggtggagat ccggttcggc
     3601 ccggacgggg cgttctactt cgtgtcgttc aacgccgggg cggtgtaccg catcgcccgg
     3661 gtgggagggg cgaaccggca accgcaggcg atcggcgcgg cggttccggc gtccgggccg
     3721 gcgccccttc aggtcctgct cgacgggacc gcctcgagcg atccggacca gcagcagacg
     3781 ctgcagttcg cctggacggc cgggcaccag ggggccaccg gcacgcaggc gacgctcaac
     3841 cacctctatc cggccggcgt ctacttcgcg cggctgacgg tgaacgacgg ccaggggggg
     3901 accgacgcga ccccggacat ccggatcgtg tccggcaacc gggccccgac cgcgacgatc
     3961 acggcgccgc tcgacgaggc gcactaccag gcgggggaca ccatcgactt ctccggcggc
     4021 ggcagcgacc ctgaggaggg gacggtcccc tgcagccgat tcacctggtc ggtcacgttc
     4081 caccatgccg gccacaccca ccctcacctg gggccgatcg aaggggtctg cggcgggtcg
     4141 ttcgtcatcc cgcggaccgg agagacctcc gccgacgtct tctacgagat cctgctggtg
     4201 gtcaacgaca ccggcgtgcc gctcggggcc gctgccgtgc tcaccggagc gacggcggtc
     4261 caggtccgcc cggacacggc gcccctgacc ttcgcgacgg cgccgcaccc gggcctcctg
     4321 gtgctgctcg acgggacgct gcagccggcc cccatcgtca ccgacgcggt ggtgaacttc
     4381 ttccgctcca tcggagcgcc ggcttcacag accggaccgg acggccgcac ctacacgttc
     4441 gcttcctggt cggacggagg cctccagagc cacacgatcg tcgtccccgc gatcccggcg
     4501 cagtacaccg ccacgttcac atgcggcgtc atcaatgagg tggcggggct ccagctggcg
     4561 cacgcgacgg ggggccaggt cgcgttgagc tggcagccga tttcggacct gtgcgccgcg
     4621 acgacggggg tgcgctatct catctacgcc tccaccaacg cccggccgtc ggtcctcccc
     4681 ggttcatttc cgaccgatcc ggccttcgtg ctgcgcggga tggtgaccgg gacctcgttc
     4741 acctatctcc ccgacccctc ggatacgttc ttcctggtcg tggcggtcgg cagcgacggg
     4801 tcggcggggc gcgcggggca ctacggcgga taggccttca gcggggcggc agaagcgaat
     4861 ctccgatcgc gcatcggacc cgccaaccgc gactcgttcc caccgcctcg gtagaaacct
     4921 atcggctcat gcccgtccgg cccgggctat ccgtggcgga tcgatcagga agcgcgatag
     4981 gtaggcggat cgttagaata cgcggcgatg cggttggccg ccctctccct tccgggcctg
     5041 ctccttgtcg gggcggcggt gctggcggcc attccttttg ccgcgcccgc cgaggctccg
     5101 agttccccgt cgaccggccg tccgctgctg gtcaccatcg acgacctgcc ggtggcgtcg
     5161 ccgaagctgc atacggatcc ggcggaccgc gaggaggtga cgcggaagct cctcgaaatc
     5221 ctgaaagcgc acaaggtccc ggcggcggtc ttcacgatct gggggcaggt gaaggggccg
     5281 aaggacaccg cactgctcga tcgctaccgc gcggcggggc acgagctggg gaatcactcc
     5341 ttcggccacc tcgatctgcc gcgcacgccg gtcgccgcgt atgtcgagga catcgagcgg
     5401 ggccgcgccg ggctcgccgg ctacctcgcg ccgcaccggc tcgaggtgcg cttcttccgc
     5461 ttcccgtatc tgcgcgaggg ggagaccggg gagcagtggg acgccgtccg caaggcgctg
     5521 cgcgcctcga agcagcgggt catgccggtg accatcgaca cgcaggactg gtcgttcgag
     5581 gagccgtacg tcgcggcggt gcggcgcggc gacgcgacgg cggcgcgcga cgtcgccgcc
     5641 gactgcctgg cggcgctcaa actcgaggtc cggcgccacg aggagcgggg ggacgatctc
     5701 ttcggccgcc agaccccgca gatcctcctc ctgcacgcca acgccgtcgg caccgccgtc
     5761 tgggacgatc tcttctcgtg gctgaagcgc accggccacc gcttcgcgac ggcggacgag
     5821 gtgctggcgg atcccgcctt cagcgaggag ccgcgctttc tcgcccgcta cggctgcagc
     5881 ctgtgggacc ggatcggcca cgagcgcgaa gcgaaggaat ccgccgcggc gatccggaaa
     5941 ctcatcgccg accaggtcga ggcgtggaac cgcggcgatc tcgtcgcctt cacgaacgtc
     6001 tacgatgacg acgcgacctt cctctccccc accgggagcg tgcgcggccg gcaggcggtt
     6061 cacgagcgtt acacgaagcg ttatcccgac cgggcggcga tggggacgct gaagctcgag
     6121 atcgacgaca tccgcccggt ctggggaatg gagatcacgc cgggagggga cgcggtgccg
     6181 ggccgcatcc acggcgcctc ggcctccggc cgctggaccc tctcctaccc ggaccgcgag
     6241 gcggcgaccg gacgcaccct gctcgtcctg tcgcgcgccg ggacccgctg gacgatcgtc
     6301 caggacgcct cgatgtgagc ggtcctccgg gggatccgtt cgccatcggg gggaagcgcg
     6361 ggcgcctgtt cctggggatc ctgctggccg gggcggcgct ccgcctcgcg tacgtggcgc
     6421 ggccgctcga tcaccgcatg cgcgccccat ggcgccaggc ggactacctg atgatcgcgc
     6481 ggaacttcgc gcgcgaggac ggctgcatcc tccatccgcg catcgactgg cgcggcgaca
     6541 cgcccggtct cgtggagatg gaggcgccga tcctcccctg gatcggcggg atgctgttcc
     6601 gcgtcttcgg cgtccacgcc gggatcctgc gcgcgctgtc atgcctcgcc gagctgctcg
     6661 cgcttctgct cttcgccggt ctggcgcggc ggatgctgcc ggacgcgggg gcgacgctgg
     6721 ccctcgcagc gttcgcgctg aacccgctcc tcgtcgatct cgcgacggcg ctccagccgg
     6781 agccgctgat gctcctgctg tgcatcgcgg tggtcgtctg cctggagcgc tatgacgccg
     6841 tgccgcccac ggagggccgg gcggtcggcc gcgaggagtg gctgctgctg tgcggggcgg
     6901 cgctcggcgg tgcgatcctc gccaaggccc ccgccgcctg cctcgggatc ctcatcgggt
     6961 gggtggtcct gcggcggatg gggacggcgg cgctcctgcg cccggccgtc tgggggacgg
     7021 cgctcctggc cctcgtccct cccgcgctgt ggtacgcgtg ggcgaagcgc tactacctgc
     7081 tctatggcaa ctcgatgggt gtgtcgaacg agtatccgtt cctcgggttc gacatgctgt
     7141 ggccgccgcg tttcttcctg ggtctcttca agtgggagac gctcggcgtc gtcacgccga
     7201 tcggctggat cctgatgatc gcttcgctgt ccgctccgtg gaaggttctc gagcggccgc
     7261 tcgtctggta cctgtcggtc tgcgtcttcc tcgtcgtggg tgcccgcacc acggccgatg
     7321 actgggcgtt ctactaccac agcctcgcgg tggcgccggc gtgcctgctc atggggatgg
     7381 ggttcgtggc cttctcccag gcgtggccgg gggcggcgcg cgccgcggcc gccgcgacct
     7441 gcctctgcct cgccggggcc accggataca tcctgtacgc gcgcgatcac cggcccgatc
     7501 tcgaggcgat gcgccggtgc ggcctgcagt tcgtggcgca catccccgaa ggagcgacgg
     7561 tcgtcacgcg cggcggcacc ctgcgcgacg aggaggggac tccggtcgcg cacaacgaat
     7621 cgatgctctt cgcctggctc gaccggcgcg gcttcaccta cggcaacgag gagctgtcgt
     7681 tcccgctgct cgaccggatc gcggctcgcg gcggacgctt ctggat
//
LOCUS       DASRIQ010000168         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2685560, whole genome shotgun sequence.
ACCESSION   DASRIQ010000168 DASRIQ010000000
VERSION     DASRIQ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685560"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFD06_04075"
     CDS             <1..658
                     /locus_tag="VFD06_04075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683148.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZN02748.1"
                     /translation="DFTASFPKVLDELDELLTGNTIWMGRTQGVGVIGAKDAVALGMS
                     GPNLRGSGVAYDVRRTEPYLEYETYEFDVPVGSTGDVYDRYLVRFEEMRQSVRILTQA
                     LKRLPDGPINIDDPKIILPPKKDVLTKMEELIQQFMVVTEGFPAPAGESYHAIEAPKG
                     ELGFYIRSTGGKAPYRLHIRSPSFISLQAIEAMSVGGLVSDLIAIIASLDPVMGEVDR
                     "
     gene            655..1143
                     /gene="nuoE"
                     /locus_tag="VFD06_04080"
     CDS             655..1143
                     /gene="nuoE"
                     /locus_tag="VFD06_04080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014768610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HZN02749.1"
                     /translation="MSDAAATLPVSVETDRKIDALLTKYPRRSSALLPALYAVQEEHG
                     WISEEAMDYVAGKIGVSPAFVAGVVSFYTMYHRKPVGKHHIQVCRTLSCMLRGSFQVM
                     ARFKEKLGVDVGQVTPDGRFSLQYVECLGACDTAPCAQVNDADVPLLDATKVDEMLAR
                     LK"
     gene            1166..2455
                     /gene="nuoF"
                     /locus_tag="VFD06_04085"
     CDS             1166..2455
                     /gene="nuoF"
                     /locus_tag="VFD06_04085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZN02750.1"
                     /translation="MAYEKLLLKNAGRENSHTIDVSLKAGGYEALKKALKGMTPEALL
                     EEVKASGLRGRGGAGFPTGMKWSFLPKTRDKPRYLCVNADESEPGTFKDRLLIERDPH
                     LVLEGIILSSFAIDCHTAFFYIRGEFQKGCRIFEQAVAEAYQKGFLGKNILGSGYDLD
                     VLVYRGAGAYICGEETALLESLEGKRGLPRIKPPFPAIVGLYGCPTIINNVETLANVP
                     FIVERGAAWFAGIGRPKNTGPKLFCLSGHVKKPGVFEAPLGIPFLELLNEHAGGMLHP
                     DRPLKAAVPGGSSMKILPANRCEVLLDFDSLAAAGTMLGSAGGMVMDSGTCMVGALLN
                     LAEFYEHESCGQCTPCREGTGWFTKILHRLETGHGRKEDPDLLIDVARRVEGNTICPF
                     GEAIAWPVQSYVAEFKSEFDDHVTKGACPFGGAAVKR"
     gene            2469..>3363
                     /locus_tag="VFD06_04090"
     CDS             2469..>3363
                     /locus_tag="VFD06_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN02751.1"
                     /translation="MPEPTPPAPPAPAAATAAPPPELPATGPDEVALLIDDKPVVVKK
                     GTTVLQAAERLGIVVPHYCYHPGLSVAGNCRMCFVEIEKMPKLQIGCATPATQGMVVR
                     TANTRVKSARESIMEFLLINHPLDCPICDQAGECRLQEYAVAYGDGESRFTESKLALA
                     KAVDIGPHVMLDQERCIQCSRCIRFCDEVTKTGELAFFSRGDHTVIGVAPGKPLDNAY
                     SGNVVDICPVGALTLKEFRFRTRVWYLKNTPSACVACARNCSVLVGTGTQREIMTTSG
                     QQDERIKRLVPRYNPEVNGHWM"
BASE COUNT          560 a         1145 c         1150 g          508 t
ORIGIN      
        1 cgatttcacc gcctccttcc ccaaggtcct ggacgagctg gacgagctgc tgaccggcaa
       61 cactatctgg atgggccgca cccagggggt cggggtgatc ggcgcgaagg acgccgtcgc
      121 gctcggaatg agcgggccga acctgcgcgg ctccggcgta gcgtacgatg tgcgcaggac
      181 cgagccgtac ctcgaatacg agacgtacga gttcgacgtg ccggtcggct ccaccggcga
      241 cgtctacgac cgctacctcg tccgcttcga ggagatgcgc cagtcggtcc gcatcctgac
      301 gcaggcgctg aaacgcctcc ccgacggtcc gatcaacatc gacgacccga agatcatcct
      361 ccctccgaag aaggacgtcc tcacgaagat ggaggagctg atccagcagt tcatggtcgt
      421 caccgagggg ttcccggccc ccgccggcga gtcgtaccac gccatcgaag cccccaaggg
      481 ggagctcggc ttctacatcc gcagcaccgg cggcaaggcg ccgtaccggc tgcacatccg
      541 ctcgccgtcg ttcatctccc tgcaggcgat cgaggcgatg tcggtcgggg ggctggtgtc
      601 ggatctcatc gccatcatcg ccagcctcga tccggtgatg ggcgaggtcg accggtgagc
      661 gacgctgccg cgacgctccc ggtgtcggtc gagaccgacc ggaagatcga cgcgctcctg
      721 acgaagtacc cgcgccggtc gtcggcgctc ctgccggccc tctacgccgt gcaggaggag
      781 cacggctgga tctccgaaga ggcgatggac tacgtcgccg gcaagatcgg cgtgtcgccg
      841 gccttcgtcg ccggagtcgt ctccttctac acgatgtacc accgcaagcc ggtggggaag
      901 caccacatcc aggtctgccg gacgctctcc tgcatgctgc gcggctcgtt ccaggtgatg
      961 gcgcgcttca aggagaagct cggggtcgac gtcggccagg tcacccccga cggccgcttc
     1021 tcgctccagt acgtcgaatg cctcggcgcc tgcgacacgg cgccgtgcgc gcaggtcaac
     1081 gacgcggacg tgccgctgct ggacgcgacg aaggtggacg agatgctcgc gaggctgaag
     1141 tgacgcgcgc ggggacgatc ggctgatggc gtacgagaag ctccttctga agaacgccgg
     1201 gcgggagaac tcgcacacga tcgacgtctc cctcaaggcc ggcggatacg aggcgctgaa
     1261 gaaggcgttg aaggggatga cccccgaggc gctcctcgaa gaggtgaagg cgtcggggct
     1321 gcgcggccgc ggcggcgccg gcttcccgac cgggatgaag tggtccttcc tgccgaagac
     1381 cagggacaag ccgcgctacc tgtgcgtcaa cgccgacgag agcgagccgg ggaccttcaa
     1441 ggatcgcctg ctcatcgagc gcgacccgca cctggtgctc gaggggatca tcctgtcgtc
     1501 gttcgccatc gactgccaca ccgccttctt ctacatccgc ggcgagttcc agaagggctg
     1561 ccggatcttc gagcaggcgg tcgccgaggc gtaccagaaa gggttcctcg ggaagaacat
     1621 cctcggcagt gggtacgacc tcgacgtgct cgtctaccgc ggcgccgggg cctacatctg
     1681 cggcgaggag acggcgctgc tggaatcgct ggaggggaag cgcgggctgc cgcggatcaa
     1741 gccgccgttc ccggcgatcg tcggcctcta cggctgcccg acgatcatca acaacgtcga
     1801 gacgctcgcg aacgtcccgt tcatcgtcga gcgcggcgcg gcgtggttcg ccggcatcgg
     1861 ccgccccaag aacaccggcc cgaagctctt ctgcctctcg gggcacgtca agaagcccgg
     1921 ggtgttcgag gcgccgctcg gcatcccgtt cctggagctc ttgaacgagc atgccggcgg
     1981 catgctgcat ccggaccggc cgctcaaggc ggccgttccc ggcgggtcgt cgatgaagat
     2041 ccttccggcg aaccgctgcg aggtgctgct cgacttcgat tcgctggccg ccgccgggac
     2101 gatgctcggc tccgccggcg ggatggtgat ggattccggc acctgcatgg tcggggcgct
     2161 cctgaacctg gcggagttct acgagcacga gtcgtgcggg cagtgcacgc cgtgccgcga
     2221 ggggaccggc tggttcacga agatcctgca ccgtctcgag accggccacg gccgcaagga
     2281 ggatccggat ctcctgatcg acgtggcgcg ccgggtcgag gggaacacca tctgcccgtt
     2341 cggcgaggcg atcgcctggc cggtgcagtc gtacgtcgcc gagttcaaga gcgagttcga
     2401 cgaccacgtg acgaaggggg cctgcccctt cggcggcgcc gccgtgaagc gctgagatcg
     2461 aggagacgat gcccgagccg accccgcccg ccccgccggc ccccgccgcc gcgaccgccg
     2521 cgccgccgcc cgagctgccg gccacaggcc ccgacgaggt ggcgctcctc atcgacgaca
     2581 agccggtcgt cgtgaagaag gggacgaccg tcctgcaggc ggcggagcgc ctcgggatcg
     2641 tcgtgccgca ctactgctac cacccggggc tttcggtcgc cggcaactgc cgcatgtgct
     2701 tcgtcgagat cgagaagatg ccgaagctgc agatcggctg cgccacgccg gcgacgcagg
     2761 ggatggtggt gcgcaccgcc aacacgcgcg tcaagtcggc gcgcgagtcg atcatggagt
     2821 tcctgctcat caaccacccc ctcgactgcc cgatctgcga ccaggcgggg gagtgccgcc
     2881 tgcaggagta cgcggtcgcc tacggcgacg gcgagagccg gttcacggaa agtaagctcg
     2941 cgctcgcgaa ggcggtcgac atcggccccc acgtgatgct cgaccaggag cgctgcatcc
     3001 agtgcagccg ctgcatccgc ttctgcgacg aggtcacgaa gaccggcgag ctggcgttct
     3061 tcagtcgcgg cgaccacacc gtcatcgggg tcgcccccgg gaagcccctc gacaatgcct
     3121 actcggggaa tgtggtcgac atctgcccgg tgggggccct gaccctgaag gagttccgct
     3181 tccgcacccg tgtctggtac ctgaagaaca ctccctcggc gtgcgtggcc tgcgcgcgca
     3241 actgcagcgt gctggtgggg acggggacgc agcgagagat catgaccacc agcggccagc
     3301 aggacgagcg catcaagcgc ttggtccccc gctacaaccc ggaggtcaac ggccactgga
     3361 tgt
//
LOCUS       DASRIQ010000169        10688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3729168, whole genome shotgun sequence.
ACCESSION   DASRIQ010000169 DASRIQ010000000
VERSION     DASRIQ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3729168"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFD06_04095"
     CDS             <1..988
                     /locus_tag="VFD06_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HZN02752.1"
                     /translation="GTSEGVSLVATADGFGAAWSDARSGHYEVFMARLDAAGRPLSEM
                     QVTREDTTLSIAPKLAWNGAEFRIGWTDARDSGSDVYVAAIDALGVRTGPETPLSSHF
                     SHTAGLGSVALAGSALGAAWSDHRDGDFEIYFGRLGCHCVDDDADGYSACAECDDQHA
                     EINPGVLEACNGEDDNCDGLADNDSDGYDSDSDAVPNACDNCRFTTNLDQADLDADGV
                     GDRCDLDDGLIMVMMRDATFVEWQMEAGAASFNLYRGAIPGLVDPDHDGAASNYGGCL
                     ATGVAGPAYGDVAVPPLGTAFLYIVTGGGPGGETSFGVASSGASRPNVSPCP"
     gene            1049..1225
                     /locus_tag="VFD06_04100"
                     /pseudo
     CDS             1049..1225
                     /locus_tag="VFD06_04100"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
     gene            1404..3635
                     /locus_tag="VFD06_04105"
     CDS             1404..3635
                     /locus_tag="VFD06_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02753.1"
                     /translation="MRVPILACFVCLSAPVSSFSAMAQVFVSAGQMGGLDTEGEGAGG
                     VAVDREGNVTVIGSFDHTGDFDPGPAAYWMTGLGIADPFIVRFDHQGRLLWAGSMSGP
                     GWDSAGDLALDAAGDILFVGSAAAGAFDADPGPGTFIISSTSGEGFLSKVDRGSGNLI
                     WARQIAHPQASSFSSSGNSVALDAGGNIHVAGSFNNGTVDCDPGPGTFNLSTTTTSYF
                     ISKLDSTGALVWARQLGGSPSSPFSLVYIHDLLVDAGGNLTMTGRFSGTVDFDPGPGT
                     FPMTASGNNDGFIARLDPAGNFVWAGRLGGNGDDVAARAALDADGSLYVTGHFQVTAD
                     FNPGPGSFLMTTTDGAFDAFVGKFTSAGGLVWARQLGGSGLQYGTTLALDAARGIITT
                     GSFNHAGDFNPGPGVFTLEPPGGPGTYGTYLSHLDPAGNFVSASVLGQRYVSAATADV
                     RGDLYLVGGFQGTDDFEPGPGTHLLTAVGRSDAYISRLGWPCPRFNDRSAYGQLVLPD
                     SCTREAFPLTADLYASTQVPIERGSRDRFFLSSTGGAFGEVLGPVFAPLVADDRIRIN
                     GIDLGLGPYAYQPDVPPIHLYTPIESTLVPLPAPELGTNLIPTGSSTMQVEILDADRN
                     IYGHTALYLVRDCGLALAGSSPTSLNWISHDAEITGTNPLFEVRYGLLSHLQQDRDFS
                     RIACLGHFSGTPATDAGPDPLPGDGYYYMGRGLDTCVEQGYGTSSLIPDPRAALDALP
                     ACP"
     gene            complement(3645..4451)
                     /locus_tag="VFD06_04110"
     CDS             complement(3645..4451)
                     /locus_tag="VFD06_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN02754.1"
                     /translation="MSTGGVGGIAHLGVLSRLQEEGVRIDALVGASAGALVAAYYAAL
                     GDPLPVLIEDAARTSLGRLAAFALHLRRLPVPGREAREHCRRLQARLEALDQVAFDGL
                     RGPVRALGVLAFDWRRLGAFFACTGSPETGTLSVGRVVRGSASIPIVFPPVRLRDGRA
                     TRFMSDGGLVRSLPLEWAFAPPLSATHALGVQLPTLRRHLDALLPARRRFLRAHESRL
                     IVVEPRIDLGRNAFRGRRGLEDVFRAGREALDDRALSLLLRWQDDPAPCT"
     gene            4508..5851
                     /locus_tag="VFD06_04115"
     CDS             4508..5851
                     /locus_tag="VFD06_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HZN02755.1"
                     /translation="MRQVVSRIIAGILLIGLGVSPSLAVDRCRAERDAVVLLHGLGRT
                     PRSMSRLEEALVGAGYEVYNVGYPSRKLRIEDLALDLDAAIEACCRGRDRYAHFVTHS
                     LGGIVLRYYLKRRPLDNVGRVVMLSPPNAGSEIVDLMRLAPILKDHIGPARGQLGTRR
                     DDPPTALGGVDFELGVIAGDRGVIPPLSWMIPGKDDGIVAVDRTRVEGMSDFVVLPRA
                     HSFIMRGADVIGQTRAFLETGRFDPIDLPGFARVDAGIFRGGQPGENGIEELAGLGIK
                     TILNLRREPETVAEERARAEAAGLRFIHVPLAPLGRPDHDSVSLILGHLTDPTNQPIF
                     VHCKRGADRTGMIVACFRILRQGWSAERAIEEAREMGLGWWQDGLNDFIDDACGAVVE
                     DLPPPRSAAQTPAAAPRACARAIGSAASADGSVKIGVPAVASKAIFPRSSSTQPR"
     gene            complement(6267..8273)
                     /locus_tag="VFD06_04120"
     CDS             complement(6267..8273)
                     /locus_tag="VFD06_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HZN02756.1"
                     /translation="MSHVPARLAPFTFALALLATAVPAHAAGLLKPKDASHGPLQIKS
                     HEVSVLINNGFAVTEVDQTFHNPHSQDLEAIYSFPLPKDASLSELSLWIDGEEMVGEV
                     VEKEKAKEIYEEEKREGRETARADKREYVAFDVHISPVRAGADTRVRLVYYQPLEIDG
                     GIGRYVYPLQEGRIDEAMQAFWDMQTRVHGRFLFDCQLRTSYPIDEVRLTGWPRPAAA
                     RQEASDTWRVRVEDVEGAASLDQDIVVYYRLAPDHPAGVELLAHRSGDGPGTFMLVIT
                     PGVDLKPIAEGVDWSIVLDVSGSMQDKLAPASEALARALDRLRPQDRFRLIVFSNQPR
                     GLIGRWTPVTPASVEQAREALRAVHSDGGTNLYEGFKQGIENLDEGRTTALVLVSDGG
                     ANIGPTQHEDFLALLATTDVRVFTFIMGQGANEPLLARLAEESGGFSMGVSTGDDLYG
                     RILQAKDKLGREALHGVEVKIDGVPITDPAPRRLPSAYFGQQIVTFGRYTKPGEAEII
                     LTARVSGEEKTWRSRAVFPARATSYPELERLWALARIKDLGRSSGKAGERSDPRKAIV
                     DLGVTYSLVTDETSMIVVRKERFDALGIDRKNRDRVDGERQARAQRSAQAPQPTRVDA
                     NQPMFTQPMHRTRGGGGAAGPELVGLFGALIGVRAWLRRRSERS"
     gene            complement(8476..9900)
                     /locus_tag="VFD06_04125"
     CDS             complement(8476..9900)
                     /locus_tag="VFD06_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN02757.1"
                     /translation="MTTRRDRGPAPAAARAHILVVDDEPNVRKVLGALLEQAGYVATL
                     AADGREALALVRSQDPDVLISDLKMPGMDGLALLRALEEAFPEIPVVLLTAHGTIEAA
                     VEAMKHGAFDFLTKPFEKDRVLEVVAKGLAQSGRSRLEFQGPWSPEAACGMVGRGSAM
                     AELRRLIERLAPSPSTVLISGETGTGKELVAEALHRMSPRAARPFVKINCGALPDTLV
                     EAELFGYESGAFTGASRAKPGRFELADGGTLFLDEIGELPAAAQVKLLRVLQDGVVDR
                     IGTTQPHRVDVRLIAATNRDLEQETARGTFRQDLLFRIRVVEIRVPPLRERLEDIPLL
                     VDFFLTKQSRRLQRPRPEVGPETLAALAARPWMGNVRELENAVERAMLLGGDSSLTPA
                     DFETGHAAAPGPVDSPAAAVDVSAPGGIKSAARAAAAVAERRMIRAAMESTGGNVTQA
                     AARLGLSRRGLQLKLKEFGLRYDF"
     gene            complement(9907..>10688)
                     /locus_tag="VFD06_04130"
     CDS             complement(9907..>10688)
                     /locus_tag="VFD06_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02758.1"
                     /translation="GGGFPDRYLRAEIAAALLAVGNQVVASLERLQAIEQARRREALA
                     AVGEMAAGLAHEVRNPLGAIQGAAQVLSGETDPVRARDMLEVIQEETARLGRVVGDFL
                     DYARAGGRRRESVDLADLARRALRSSDAAGDGLQGVVRVAPGTPPAAGDPDQLQRAIG
                     NIVRNACEAAGRGGRLTIDVAPEGAHRVSMRFEDDGPGIPPENVPRLFLPFFTTRPGG
                     TGLGLALVHRVMEAQGGEVRVDVRPGRGAVFTLVLPALDVH"
BASE COUNT         1621 a         3720 c         3694 g         1653 t
ORIGIN      
        1 tgggacgtcc gagggggtct ccctggtggc gaccgcggat ggtttcgggg ccgcctggag
       61 cgatgcccgt tccgggcact acgaagtgtt catggcgcgt ctcgatgcgg ccgggcgacc
      121 gctgtccgag atgcaggtga cccgggaaga taccacgctg tcgatcgcgc cgaagctcgc
      181 ctggaacggc gcggagttcc gcatcggttg gaccgatgca cgcgacagcg gcagcgatgt
      241 ctacgtcgcc gcgatcgatg cgctcggagt gcggaccggc cccgagacgc ccctcagcag
      301 ccacttctcg cacaccgccg gcctgggctc cgtggccctg gcagggagcg ctctcggcgc
      361 ggcctggtcc gaccatcgcg acggggattt cgaaatctat ttcgggcggc tcgggtgcca
      421 ttgcgtcgac gacgatgccg acggctacag cgcctgtgcc gagtgcgacg accagcacgc
      481 ggagatcaac ccgggcgtgc tcgaagcttg caacggcgag gacgacaact gcgacgggtt
      541 ggccgacaac gacagcgatg gatatgactc cgacagcgac gcggtgccga acgcctgcga
      601 caactgccgt ttcacgacca acctcgatca ggccgacctc gacgcagacg gggtcggcga
      661 ccgctgcgat ctggacgatg ggttgatcat ggtgatgatg cgcgacgcga cgttcgtcga
      721 atggcagatg gaggcaggcg ccgcctcgtt caatctctac cgtggcgcca ttccggggct
      781 cgtcgacccg gaccacgacg gcgcggcgtc gaactacggc ggctgcctgg cgacaggtgt
      841 ggcgggccct gcgtacggcg atgtggcggt gcccccgctg gggaccgcgt ttctctacat
      901 cgtgaccggg ggcggcccgg gaggcgaaac cagcttcggc gtcgccagca gcggcgcttc
      961 gcgcccgaac gtttctccct gcccctgatc tccctgcgcg ccgcgacgcc gcgctccctg
     1021 cccgcgaagc gctcgcgcga ctccgggagg tgcgaaagcc catggtcgat cggtggtggt
     1081 ccgcatcaac gatcgcggtc cctatgtcga gaagcgggtg atcgacctct cctacgccgc
     1141 cgcccgggag ttgcagttca tcgacgacgg tacgacccgg gtgcgcctcg aagtgctcga
     1201 tcaccgtgcc gacaaacgtc cgtgaagctc cagcgcggga agatggcgtc gacgaagatc
     1261 cgcgcgatgg ccggtctcct gagcgcgcct tcgatcggcg gcagcaggac cacggcgccg
     1321 ctgcgcgcca tgaattcgtt gttgtttgtg cgtcctgcgt caccgatcct ataatcgccg
     1381 caaagctcga cgggaggtga cgcgtgcgtg tcccaatcct cgcctgcttc gtgtgcctct
     1441 ctgcgcccgt ctcttctttc tccgcgatgg cccaggtctt cgtcagtgcc ggacagatgg
     1501 gcggcctcga tacggaaggg gaaggagccg gcggcgtcgc cgtggaccgg gagggcaacg
     1561 tcaccgtgat cggatcgttc gatcacaccg gtgacttcga tccgggcccc gccgcttact
     1621 ggatgaccgg tctaggaatt gcggacccct tcatcgtcag gttcgaccat cagggccgtc
     1681 tcctctgggc cggcagcatg agcgggcccg ggtgggattc cgccggcgac ctggccctgg
     1741 acgcggccgg agacatcctg ttcgtgggca gcgccgccgc cggcgccttc gacgccgacc
     1801 ccggtcccgg gacgttcatc atcagcagca cctcggggga gggattcctc tccaaggtgg
     1861 accgcggttc ggggaatctg atctgggcca ggcagatcgc ccatccacag gcgagctcgt
     1921 tctcctcctc cgggaactcc gttgcgctgg atgcgggagg caacatccat gtcgccgggt
     1981 ctttcaacaa tggcacagtg gattgcgatc ccggaccggg cacgttcaac ctgagcacga
     2041 cgaccacttc ctacttcatc tccaagttgg acagcaccgg cgccctcgtc tgggccaggc
     2101 agctgggcgg ctcgccttcg tccccttttt ccctcgtgta tatccacgac ctgctggtgg
     2161 atgcaggggg aaatctgacc atgaccggac gtttctccgg gaccgtcgac ttcgatcccg
     2221 gaccgggaac gtttccgatg acggcgtcgg ggaacaacga tggtttcatc gcccggctgg
     2281 acccggcagg gaacttcgtc tgggcaggcc ggctgggagg gaacggggac gacgtggccg
     2341 cacgcgctgc gctggacgca gacggcagcc tctacgtgac cggtcatttc caggtgaccg
     2401 cggacttcaa tcccggcccc ggctccttcc tcatgacgac gacggacggc gccttcgacg
     2461 ccttcgtcgg caagttcacc agcgcgggtg gtctggtctg ggccaggcag ctgggcggta
     2521 gcggcctgca atatggcacc accctcgctc tggatgccgc ccgcggaatc atcacgacgg
     2581 gaagcttcaa tcacgccggt gacttcaatc ccggtcccgg cgtgttcacc ctggagccac
     2641 cgggcgggcc tggaacctac gggacctatc tttcgcatct cgatccggcg ggcaacttcg
     2701 tgtcggccag cgtcctgggg cagcgctatg tgtccgccgc gacggcggac gtcaggggag
     2761 acctgtatct cgtcggaggg ttccaaggga cggatgactt cgagcccggt ccggggacgc
     2821 acctcctgac cgcggtgggg cgcagcgacg cttacatctc caggctcgga tggccctgtc
     2881 cccgcttcaa tgacaggagc gcgtacggtc agctcgtcct cccggattcg tgcacccgcg
     2941 aagcctttcc cctgacggcc gacctctacg cctcgacgca agtcccgatc gagcggggtt
     3001 cgcgcgatcg gttcttcctt tcgtcaaccg gtggcgcctt cggcgaggtc ctcggcccgg
     3061 tcttcgctcc gctcgtcgcc gacgacagga ttcgcatcaa cgggatcgac ctggggctcg
     3121 ggccgtacgc ctaccagccg gacgtgcctc ccatccacct ctacacgccg atcgaatcga
     3181 ctctggtccc gctcccggcc ccggagctgg ggaccaatct catccctacc ggctccagca
     3241 cgatgcaggt cgaaatcctc gacgccgatc ggaacatcta cggccatacg gcgctctacc
     3301 tcgtgcgcga ctgcggactc gcgctggcgg ggagctcgcc gaccagcctc aactggatca
     3361 gccacgatgc ggagatcact gggacgaatc ctctgttcga ggtgcggtat ggcctgctgt
     3421 cccatctgca gcaggaccgg gatttttcgc gcatcgcgtg cctcgggcac ttctccggca
     3481 cgcccgccac ggatgccggg ccggatccgc tccccggcga cggctactac tacatgggac
     3541 gcggcctcga cacctgcgtc gagcagggtt acggaacgtc gagtctcatt cccgacccca
     3601 gggccgcgct cgatgcgctg ccggcgtgcc cctgattccg gggctcaggt gcagggggcg
     3661 gggtcgtcct gccagcggag caggagggac agggcgcgat cgtcgagcgc ctcgcggccg
     3721 gcacggaaca cgtcttcgag cccccgccgc ccccggaatg cgttgcgccc caggtcgatg
     3781 cgcggctcga cgacgatgag acggctctcg tgcgcgcgca ggaaccggcg gcgcgccggc
     3841 agcagcgcat ccaggtggcg gcggagcgtg ggcagctgga ctccgagcgc gtgggtcgcc
     3901 gacagcggcg gcgcgaaggc ccattcgagc ggcagcgagc gcaccaggcc gccgtcgctc
     3961 atgaagcggg tcgcgcgacc gtcacgcaga cggaccggag ggaacacgat cggaatcgag
     4021 gcgctgcccc gcaccacgcg gccgaccgag agcgtgccgg tctccggcga gccggtgcag
     4081 gcgaagaagg ctcccaaccg gcgccagtcg aaggccagga ctcccagcgc ccgcaccggt
     4141 ccgcgcagtc catcgaacgc gacctgatcg agcgcttcga gacgcgcctg cagacggcgg
     4201 cagtgctcac gcgcctcgcg gccgggcacc ggcaggcggc gcagatgaag cgcgaaggcc
     4261 gcgaggcgcc cgaggctggt ccgggcggcg tcctcgatca ggacagggag gggatcgccg
     4321 agcgccgcgt agtacgccgc caccagcgcc ccggcgctcg ccccgacgag cgcgtcgatg
     4381 cgcacgccct cttcttgtaa gcggctcagg acccccaggt gggcgattcc gcccaccccg
     4441 ccggtcgaca tgacgacggc cagcctaggg ctggacaaag tgcgcctcga ggagaatgct
     4501 gagcgccatg cggcaggtcg tttcgaggat catcgccggg attctgctga tcggccttgg
     4561 tgtctcaccg tcgttggccg tcgatcgctg ccgcgccgag cgggacgccg tggtcctgct
     4621 gcacggcctg ggacgcaccc cgcggtcgat gagccggctc gaagaggcgc tggtgggcgc
     4681 gggatacgag gtctacaacg tcggctatcc ctcccgcaag cttcggatcg aggatctggc
     4741 gctggacctg gacgccgcca tcgaggcgtg ctgccgcggc cgcgatcggt acgcgcactt
     4801 cgtcacgcac tcgctcggag ggatcgtcct ccgttattac ctgaagcggc ggccgctcga
     4861 caatgtcggc cgcgtcgtga tgctgagccc gccgaacgcc ggcagcgaga tcgtcgatct
     4921 catgcggctg gcgcccattc tcaaggatca cattggccct gcccgcgggc agctcgggac
     4981 gcggcgggac gatccgccga cggcgctggg aggcgtcgat ttcgagctcg gcgtcatcgc
     5041 cggggatcgt ggtgtcatcc ctcccctgtc ctggatgatc ccggggaaag acgacgggat
     5101 cgtggccgtc gacaggacac gcgtggaggg catgagcgat ttcgtcgtgc tcccccgcgc
     5161 gcactcgttc atcatgcgcg gagcggacgt catcggccag acacgcgcgt tcctggagac
     5221 aggacggttc gacccgatcg atcttcccgg cttcgcgcgc gtcgatgccg gaatcttccg
     5281 gggaggccag ccgggcgaga acggcatcga agagctggcc ggactgggga tcaagaccat
     5341 tctgaacctc cgccgggaac ccgagacggt cgccgaggag cgcgcccggg cggaagccgc
     5401 cggcttgagg ttcatccatg ttcctctggc gcccctcggc cgtccggacc acgactccgt
     5461 cagcctgatc ctcgggcacc tgacggaccc gacgaatcag ccgatcttcg ttcattgcaa
     5521 gcgcggggcc gatcgcacgg ggatgatcgt cgcctgcttt cggatcctga ggcaaggctg
     5581 gagcgcggag cgggcgatcg aggaggcgcg ggagatggga cttggctggt ggcaggacgg
     5641 cctgaacgac ttcatcgatg acgcctgcgg ggccgtggtc gaagatctcc cgccgcccag
     5701 aagcgccgcg cagacgccgg cggcggcccc cagggcgtgc gccagagcga tcggttccgc
     5761 cgccagcgcc gacggatccg tgaagatcgg cgtgccggcg gtcgcctcga aggcgatctt
     5821 tcccaggtcc agcagcacgc aacctcggta gacgaagggc agggaacggc gcccctcgcg
     5881 gctctcgttc cgtgcggcca gcagggcgcc tgcgacccag atcccgttca gcgcgccgga
     5941 caggccgcga taggaatcga gtccgggctc caggagcagc agccctccgc ccaccgcggc
     6001 acaggagccg aacagcaccg gccagaagcg acggccgagc gcgtcttcga acaggaagcc
     6061 gatcgcgacg agaggcagca ggttccagat cctgtgatac gtgctgcagt gcacgaagtg
     6121 ggccgtcacg atcctccaga cctcaccacg caggatggcg gcgcggtcgt attcgagcag
     6181 gcgggccgcg gctggcgccg cggcggcgag gatcgccgcc gcgagcgcca gtgacttcaa
     6241 cggcagccgg gacgagacca gccgcttcac gaccgttcgc ttctccggcg cagccaggca
     6301 cggacgccga tgagggcgcc gaacaggccg accagctccg ggcctgcggc gccgcctccg
     6361 ccgcgcgtcc gatgcatcgg ctgggtgaac atcggctggt tcgcgtcgac gcgcgtgggc
     6421 tgcggtgcct gcgccgagcg ctgggccctg gcctggcgtt cgccgtccac gcgatcgcgg
     6481 ttcttgcgat cgatcccgag cgcgtcgaac cgttccttgc gaacgacgat catcgacgtt
     6541 tcgtcagtga ccagcgagta cgtgacgccg agatcgacga tggccttccg gggatcggag
     6601 cgctcgccgg cttttccaga gctacggccg aggtctttga tgcgcgccag cgcccagagg
     6661 cgctccagct cggggtagct ggtcgcacgc gccgggaaga cggcgcgcga gcgccaggtc
     6721 ttctcttctc ccgagacgcg cgccgtcagg atgatttcgg cttcaccggg cttggtgtag
     6781 cggccgaacg tcacgatctg ctgtccgaag taggcgctgg gaagtcgccg gggggccggg
     6841 tccgtgatcg gcacgccgtc gatcttcacc tcgactccgt gaagcgcctc gcggcccagc
     6901 ttgtccttcg cctgcaggat gcggccgtac aggtcgtcgc ccgtcgaaac ccccatcgag
     6961 aagccgcccg actcttccgc gaggcgcgcc agcaggggct cgttggctcc ctgtcccatg
     7021 atgaaggtga agacgcggac gtcggtcgtg gcgagcagcg cgaggaagtc ctcgtgctgc
     7081 gtcggaccga tgttggcgcc gccgtcgctc accaggacga gggccgtggt ccgtccctcg
     7141 tcgagattct cgattccctg cttgaagccc tcgtagagat tggtgccacc atccgaatga
     7201 acggcgcgca gggcctctcg ggcctgctcg acgctcgctg gggtcacggg cgtccaccgg
     7261 ccgatgaggc cgcgcggctg gttcgagaag acgatgaggc ggaagcgatc ctgcggccgc
     7321 agccgatcga gggcccgggc gagcgcctcg gacgccggcg ccagcttgtc ctgcatgctg
     7381 ccggagacgt cgagaacgat gctccagtcg accccttccg cgatcggctt caggtcgacg
     7441 cccggggtga tcaccagcat gaacgttccc ggtccgtccc ccgagcggtg cgcgagcagc
     7501 tccacgcccg cgggatggtc cggcgccagg cggtagtaca cgacgatgtc ctggtcgagc
     7561 gacgcggctc cttccacgtc ctcgacgcgc acgcgccagg tgtcgctcgc ctcctggcgc
     7621 gccgccgccg gccgcggcca gccggtgagg cggacctcgt cgatgggata cgacgtgcgc
     7681 agctgacagt cgaacagaaa ccgtccgtgc acgcgcgtct gcatgtccca gaaggcctgc
     7741 atggcctcgt cgattcgccc ctcctgcagc ggatagacgt agcgcccgat tcctccgtcg
     7801 atctcgagcg gctggtagta gacgaggcga acgcgggtgt ccgcgccggc gcgtacgggc
     7861 gagatatgga cgtcgaaggc cacgtactcg cgcttgtcgg cccgcgccgt ctcgcggccc
     7921 tcgcgtttct cctcctcgta gatctccttc gccttctcct tctccacgac ctcgccgacc
     7981 atctcttcgc cgtcgatcca gaggctcagc tcggagaggg aggcgtcctt gggcagcggg
     8041 aagctgtaga tggcctcgag gtcctgggag tgcgggttgt gaaaggtctg atcgacctcg
     8101 gtgaccgcga aaccgttgtt gatgaggacc gacacctcgt ggctcttgat ctggagcgga
     8161 ccgtgcgagg cgtccttcgg cttgagcagt ccggcggcgt gcgccggaac cgccgtcgcc
     8221 agcagcgcga gcgcgaacgt gaacggggcg aggcgtgcag gaacgtggga catcaccgac
     8281 cccctgtcgg ccgggggaaa cgccggcgcc gtgccaggcg ccgcgggtcc ggccggcgcg
     8341 accctggcgc aacccgcgtg ccgccgatgc cggccgaccc gggtcgttgt ggccgaagga
     8401 cttaggcggg atcccccgcg gcggtgcgca gcgcggttcg cagcgcgccg cggtggatgc
     8461 aaagagtcgt tcgcttcaga agtcgtaccg caggccgaac tccttgagct tcagctgcaa
     8521 tccgcgccgc gacaggccga gccgcgccgc cgcctgggtc acgttgcccc cggttgactc
     8581 catggccgcg cggatcatgc ggcgctcggc gaccgcggcg gcggcgcgcg ccgccgactt
     8641 gattccgccc ggggcggaga catcgaccgc cgcagcaggg gagtccaccg ggcccggagc
     8701 ggccgcatgg cccgtttcga aatcggccgg ggtgagcgac gagtcccctc cgagcagcat
     8761 cgcccgctcg acggcgttct cgagctcgcg cacgtttccc atccagggac gggcggccag
     8821 tgcggccagc gtctccgggc cgacctcggg ccgcggacgc tgcagacgcc gcgactgttt
     8881 ggtcaggaag aagtcgacca gcagcgggat gtcctcgagg cgctcgcgca gcggcggcac
     8941 gcggatctcg accacccgga ttcggaacag caggtcctgg cggaacgtcc cacgcgccgt
     9001 ctcctgctcc agatcgcggt tggtggccgc gatcagtcgg acatcgacgc ggtgcggctg
     9061 cgtggtcccg atgcgatcga ccacgccgtc ctgcaggacc cgcagcagct tgacctgggc
     9121 cgctgcggga agctcgccga tctcgtcgag gaagagcgtc ccgccatcgg cgagctcgaa
     9181 gcgtcccggc ttggccctcg acgctccggt gaacgcgccg ctttcgtaac cgaacagctc
     9241 cgcctccaca agggtgtcgg gaagggcgcc gcaattgatc ttgacgaagg gacgggcggc
     9301 tcgcggcgac atccggtgca gcgcctcggc caccagctcc ttgccggtgc cggtctcgcc
     9361 ggagatcagc accgtggagg ggctcggcgc caggcgctcg atcaaccgcc gcaactcggc
     9421 catcgccgac ccacgcccga ccatcccgca ggccgcctcg ggagaccacg gtccctggaa
     9481 ctcgagccgc gagcgccccg actgggccag gcccttggcg accacctcca gcacgcggtc
     9541 cttctcgaac ggcttggtga ggaaatcaaa ggctccgtgc ttcatggcct cgacggccgc
     9601 ctcgatggtc ccgtgcgcgg tgaggagaac gaccgggatc tccgggaacg cctcctcgag
     9661 ggcgcgcagg agggcaagcc cgtccatccc gggcatcttc agatcggaga tgaggacgtc
     9721 gggatcctgg gagcgcacga gggccagcgc ctcgcggccg tcggccgcca gggtcgccac
     9781 gtatccggcc tgctccagaa gggcgccgag gaccttgcgg acattgggct cgtcgtccac
     9841 gacgaggatg tgagcccttg cggcggccgg cgccggacct ctgtcccgtc gggttgtcat
     9901 tgcgcctcag tgtacgtcga gcgccggcag gacgagggtg aagaccgcgc cgcggccggg
     9961 acggacgtcc accctcactt cacccccctg cgcttccatg acgcgatgca cgagcgcgag
    10021 gccgaggccc gtgccgcccg ggcgcgtggt gaagaacggc aggaagaggc gcggcacgtt
    10081 ctccggcggg atgcccgggc cgtcatcctc gaatcgcatc gagacgcgat gggccccctc
    10141 cggcgcgacg tcgatggtca ggcgcccccc ccgcccggcg gcctcgcacg cgttgcgcac
    10201 gatgttgccg atggcgcgtt gcagctggtc gggatcgccc gcggccggcg gcgtccccgg
    10261 ggcgacgcga accaccccct gcaggccgtc gccggcggcg tcggaggagc gcagggcgcg
    10321 gcgggcgagg tcggcgagat cgaccgactc gcgccggcgt ccccccgccc gggcgtaatc
    10381 gaggaagtcg ccgaccacgc gccccagtct cgccgtctct tcctggatca cctccagcat
    10441 gtcgcgggcg cggaccgggt cggtttcccc tgacaggacc tgcgccgcgc cttgaatcgc
    10501 cccgagcggg ttgcgcactt cgtgcgccaa accggccgcc atctctccca ccgccgcaag
    10561 cgcctccctg cggcgcgcct gctcgatcgc ctgcagccgc tcgagcgagg ccacgacctg
    10621 attgccgacg gcgagcaggg cggcggcgat ctccgctcgg aggtatcgat ccggaaagcc
    10681 gccgccaa
//
LOCUS       DASRIQ010000170         8501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2143169, whole genome shotgun sequence.
ACCESSION   DASRIQ010000170 DASRIQ010000000
VERSION     DASRIQ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143169"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFD06_04135"
     CDS             complement(<1..491)
                     /locus_tag="VFD06_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02759.1"
                     /translation="MTDEFLMQDASIRHPWFAAGSTAADSANPFLLPDIDPTNPPMES
                     MERRQSTVKHLNLELEKLEQRIAPGGVGLLGLGNCGSASGSKDDGSKGSNGDDNGSKD
                     ASSGTKDNGSNGSEDHSASEGDDGSKDCASRDDDDCDDDDDDDGGSKDDDCDDDDDDD
                     DGGS"
     gene            658..981
                     /locus_tag="VFD06_04140"
     CDS             658..981
                     /locus_tag="VFD06_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02760.1"
                     /translation="MWLLVVAAFGMLVPNGLFVWWLARHSFDLLAPLSDPLALAFVLD
                     VFASTFLLCWLFARKPFGPYRWGWFLGLSLLGTLCFGIPMYLWLNWRSVPAPRPAFAD
                     WWRAA"
     gene            1153..2919
                     /gene="kaiC"
                     /locus_tag="VFD06_04145"
     CDS             1153..2919
                     /gene="kaiC"
                     /locus_tag="VFD06_04145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015185582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HZN02761.1"
                     /translation="MTRPAGHLKAARPKLAKAPSGIQGLDEITGGGLPQGRPTLVCGA
                     AGCGKTLLAMEFLVRGATQHGEPGVFMAFEETAEELTQNVRSLGFDLDALCRQKKLAV
                     DFVRIERSEIEETGEYDLEGLFIRLDHAIDSIGAKRVVLDTVEALFGGLSNTLILRAE
                     IRRLFRWLKDKGVTAIITAERGDGTLTRHGLEEYVTDCVILLDHRVDDQVSTRRLRVV
                     KYRGSSHGTNEYPFLIDEDGISVLPITSLGLNHAVTDERVPTGVARLDAMLDGQGYYR
                     GSTVLVSGTSGSGKSSMAAHFVDTASRRGERSLYFAFEESPGQIVRNMRSIGLDLEPW
                     VKKGLLHFQASRSSLYGLEMHLAVMHKLVKEIEPRLVILDPINNLSRAGGTAKDVLST
                     LTRLVDFLKVQGITTMLTNVTTQDHVEETDSDISSLVDTWLLLRDLELNGERNRAMYV
                     LKSRGMPHSNQIREFLLTSRGIELNDVYLGAEGVLTGSARLAQEAREKAATLKRQQEV
                     EAKQRELERRREALEARITALRKEFEAESQELERRIVEDKARTDVARMDKVRMGISRK
                     SEDGSGVPRAVARSRRPQEHQA"
     gene            2916..3257
                     /locus_tag="VFD06_04150"
     CDS             2916..3257
                     /locus_tag="VFD06_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HZN02762.1"
                     /translation="MKRTATVAVKKVTELRSDAAVWELRLYVAGQSPKSVAAFTNLKK
                     LCDEHLAGKYKIEVIDLVQQPQLAAGDQIVAIPTLVRKLPEPLRKIVGDLRDTERALV
                     GLNLRPVRSEA"
     gene            3263..3622
                     /locus_tag="VFD06_04155"
     CDS             3263..3622
                     /locus_tag="VFD06_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HZN02763.1"
                     /translation="MSPPVKRSIAATTRAFEKMLQEPPGQQQYVLRLYVSGRKPRSAR
                     AITTLTALYHQHLEGRCDLEIIDVDEDPERAIQAQIFALPTLVKELPDPVRRWVGDFA
                     DEDRILVGLDLKRRLQT"
     gene            3622..5373
                     /locus_tag="VFD06_04160"
     CDS             3622..5373
                     /locus_tag="VFD06_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02764.1"
                     /translation="MERDASPPKPGTAPSPGAPPGDYGNGRGSTVDGLKAANAALRAR
                     LDEAEETIRAIQQGAVDAFVLEELGRHRVYTLEGGDRPYQVFVEQMQQGVATLHLDGT
                     VMYGNRRLAEMLKTPRESLIGTSLRGFVAELDKPLYDDLLKQGQGRSARGEIRLRPAE
                     GEEFPAYLTLSLLPGESMVGLFVTDLTVQKHHEALAAAQEALREADRRKNEFIAMLAH
                     ELRNPLAPIRNAAGILKRTADDPKMVEEIGGMLERQIGLMVRLVDDLLDVSRITRNRV
                     ELRRQRLELASVLEQAVESIRPLCRKARHHITVALPPEPVYLDADPVRLTQVFTNLLD
                     NACKYMEPGGRVRLSAEVTPARDDAPGEVVVRVRDEGIGIPAEALSRIFEMFAQIDTS
                     LERRNAGLGIGLSLVNSLVELHGGKVEARSEGKGQGSEFVVRLPTVAEGCDGLSAQEG
                     EKSMKRYRMLVVDDNRDSAESLALLLKLTGHDTHTALDGEAAIRAAAENQPDVVLLDI
                     GMPKLNGYDACKRIRSEPWGRDMILIAQTGWGQDDDRRRTEEAGFDGHVVKPVDPEDL
                     IRMVARLSTARRPEQQS"
     gene            5675..7234
                     /locus_tag="VFD06_04165"
     CDS             5675..7234
                     /locus_tag="VFD06_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN02765.1"
                     /translation="MTLAQYLERRSGPRRVPIGPEDAPAALWNPLLPGLPRLPETNRF
                     VRSPATGGAKPPAAEDDIAYAPVTRLARWIETRAITSERLTGIYLERIKRFDGRLRSV
                     ITLTSERALERARQADREIAAGKYRGPLHGIPYGVKDLLDTKGIPTTYGAEPFRDRVP
                     AADAAVVRRLDDAGAVLLAKLSLGALALNDIWFGGQTMNPWLPEEGASGSSAGPGAAT
                     AAALVPFSIGSETGGSIVSPAMRCGLTGLRPTFGRVARAGAMTLCWSLDKLGPMTRGV
                     EDAFLVLQAIDGPDAGDVSSVASRLDFDATASVSGLRIGFIPKWMDEPPATDVDREAL
                     RVMRGLGMQVREVTLPDWPFSSLYPILFAEGAASFEELALDGRLHDLRMQVKDAWPNL
                     FRQARFLSAVDLVQADRLRRKVSMLMAGIFRDLDLLLVPSLRDEQLTITNFTGHPSLT
                     LRAGFVKVSEARSDWAPDPERPLPKFDPPRRVPHGITLIGRLFDDGLLGRAGIALEKA
                     FGVAAEKPPRF"
     gene            7383..>8501
                     /locus_tag="VFD06_04170"
     CDS             7383..>8501
                     /locus_tag="VFD06_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN02766.1"
                     /translation="MAEPRPQAEAALVRGLGTLDVGLITFNALVGSAIFIAAAIVPRA
                     APHAWLILPLWIAGGALCLAGVTTYAELGTMFPKAGGQYQYLKEAYGPLWGFLFGWTA
                     FLVIQCGAIAYISVAGADGLGRIWPALGSSHTLLTIPLGSATWRVQGSQAGGAALIAG
                     ITFVNYFGLRAGAGLQNAIGAVKIVSLLVFIGFGLAVTPTAPDPWSEPLPQGSLLTPL
                     AVAMIGVLWCYDGFYQASFSAGEIRDPAKSLPRGMILGTAGTVVLYALVNIVYLRALP
                     PKALGEAPRVAEAAAAALFGEGPGRFMSAAVLVSILGCLATCILTAARIYLPMAQDGL
                     FFRSLARVHPVYRTPGACLVAQAAWSSVLAFSGSYEQLG"
BASE COUNT         1287 a         2970 c         2915 g         1329 t
ORIGIN      
        1 gacccgccgt cgtcgtcatc gtcatcgtcg tcgcagtcgt cgtccttcga cccgccgtcg
       61 tcgtcatcgt cgtcgtcgca gtcgtcgtcg tccctggaag cgcagtcctt ggagccgtcg
      121 tccccttcgg acgcgctgtg atcttcgctg ccgttggacc cgttgtcctt ggtgccggag
      181 ctggcgtcct tggagccgtt gtcgtctccg ttcgagccct tggatccgtc gtccttggat
      241 ccgctggccg agccgcagtt cccgagcccg agcaatccga cgccgccggg cgcgatgcgc
      301 tgctcgagtt tttccagttc gagattcagg tgcttcacag tgctctgcct cctttccatg
      361 ctttccatcg ggggatttgt aggatcgatg tcagggagga ggaatgggtt cgcactgtcg
      421 gcggccgtgg atcctgccgc gaaccatgga tgcctgattg atgcgtcctg catgaggaac
      481 tcatcagtca ccccttcgat cctgattggt cggtcgtgcg agtatcacgt cagcgcgccg
      541 ggtcgacaat gcgcgaatgt cctgtcatca agactcgtga atctacggat cgacacgtag
      601 aaaccgccgt gacattcgga cttttcggca tgccggctgg caccaggagg ggaaaccatg
      661 tggcttctcg tcgtcgccgc cttcggcatg ctcgtgccga acggcctctt cgtgtggtgg
      721 ctggcgcgcc actcgttcga cctgctcgcg ccgctgtcgg atccgctggc gctcgcattc
      781 gtcctcgacg tcttcgcctc caccttcctg ctctgctggc tgttcgcccg caagccgttc
      841 ggcccgtacc gctgggggtg gttcctgggg ctgtcgctcc tcgggacgct ctgcttcggg
      901 atcccgatgt atctctggct caactggcgc agcgtccctg cgccgcgccc ggccttcgcc
      961 gactggtggc gcgccgccta gaatggccgt cgcggccgag cccccgcagc actgcgaggc
     1021 ttgccgtacg ggcattccgt cacctttcta cgaacttcta cggacgatcg tccggccgcg
     1081 gctgaggtac gctcactggc aaccctaggt gcagaaggct cggcgcaaac aggcctgacg
     1141 taaggagacc cgatgacgag acctgccggc cacctcaagg ccgcacgccc gaagctggcg
     1201 aaggcgcctt ccggcattca ggggctcgac gagatcaccg gcggagggct gccgcagggg
     1261 cgtccgacgc tcgtctgcgg cgccgccggc tgcggcaaga cgctcctggc gatggagttc
     1321 ctggtgcgcg gcgccaccca gcacggggag ccgggcgtct tcatggcgtt cgaggagacc
     1381 gccgaagagc tgacccagaa cgtccgctcg ctcggcttcg acctggacgc cctgtgccgt
     1441 cagaagaaac tcgccgtcga cttcgtgcgc atcgagcgca gcgagatcga ggagaccggc
     1501 gaatacgacc tcgaggggct gttcatccgg ctggaccacg ccatcgactc gatcggcgcg
     1561 aagcgcgtgg tgctcgatac cgtggaggcg ctgttcggcg gcctgtcgaa caccctcatc
     1621 ctgcgcgccg agatccggcg gctcttccgc tggctcaagg acaagggggt gacggcgatc
     1681 atcaccgccg agcgcggcga cgggacgctc acccggcacg gcctcgaaga gtacgtcacc
     1741 gactgcgtca tcctgctcga ccaccgcgtg gacgaccagg tctcgacgcg ccggctgcgg
     1801 gtggtcaagt accgcggatc gtcccacggc acgaacgagt accccttcct catcgacgag
     1861 gacggcatca gcgtcctgcc gatcacctcg ctcggcctga accacgcggt gaccgacgag
     1921 cgggtcccga ccggcgtcgc ccgcctcgac gccatgctcg acgggcaggg gtattaccgc
     1981 ggcagcacgg tgctcgtgag cggcacctcc gggagcggca agagcagcat ggcggcccac
     2041 ttcgtggaca cggcgagccg acgcggcgag cgctccctct acttcgcctt cgaggagtcg
     2101 ccggggcaga tcgtccgcaa catgcgctcc atcgggctcg acctcgagcc gtgggtgaag
     2161 aagggactgc tgcacttcca ggcctcgcgc tccagcctgt acgggctgga gatgcacctg
     2221 gcggtgatgc acaagctggt gaaggagatc gagccgcgtc tcgtcatcct cgacccgatc
     2281 aacaacctgt cgcgggcggg cggaaccgcg aaggacgtgc tgtcgaccct cacccgcctg
     2341 gtggacttcc tgaaggtgca ggggatcacg acgatgctga ccaacgtgac cacccaggat
     2401 cacgtcgagg agaccgacag cgacatctcg tcgctcgtgg acacctggct cctgttgcgg
     2461 gacctcgagc tgaacgggga gcggaaccgc gcgatgtacg tcctgaaatc gcgcggcatg
     2521 ccgcactcca accagatccg cgagttcctg ctgaccagcc gcggcatcga gctgaacgac
     2581 gtctacctcg gcgccgaggg tgttctgacc gggtcggcgc gtctggcgca ggaggcgcgc
     2641 gagaaggccg ccacgcttaa gcggcagcag gaggtcgagg cgaagcagcg cgagctggag
     2701 cgcaggagag aggcgctcga ggcgcggatc acggcactgc gcaaggagtt cgaagcggag
     2761 tcgcaggagc tggagcgccg catcgtcgag gacaaggcgc gcaccgatgt cgcgaggatg
     2821 gacaaggtgc gcatgggcat cagtcgcaaa tcggaagacg gctcgggcgt tccccgggcg
     2881 gtggcccggt cgcgcaggcc gcaggagcac caggcatgaa gagaacagcc acggtcgcgg
     2941 tgaagaaagt gaccgagctc cgctcggacg ctgccgtctg ggagctgaga ctctacgtcg
     3001 cggggcagag ccccaagtcg gtggccgcct tcacgaacct caagaaactc tgtgacgagc
     3061 acctcgccgg gaagtacaag atcgaggtca tcgacctggt gcagcagccg cagctggcgg
     3121 caggggatca gatcgtcgcg atcccgacgc tggtgcggaa actccccgaa ccgctgcgca
     3181 agatcgtcgg cgacctgcgc gacaccgagc gggccctcgt cggtctcaac ctgcgtccgg
     3241 tgcgatcgga ggcgtgagcc gaatgtcgcc tcccgtgaag cgctccatcg cggcgacgac
     3301 gcgggcgttc gagaagatgc tgcaggagcc gcccgggcag cagcagtacg tgctgcgtct
     3361 ctacgtcagc ggccggaagc cgcgctcggc ccgcgcgatc accacgctga ccgccctcta
     3421 ccaccagcac ctcgaggggc ggtgcgatct ggagatcatc gacgtggacg aggacccgga
     3481 gcgggcgatt caggcgcaga tcttcgcctt gccgaccctg gtcaaagagc tcccggaccc
     3541 ggtgcgccgg tgggtcggtg atttcgccga cgaggaccgc atcctcgtcg gcctcgacct
     3601 gaagcggagg ctccaaacgt gatggaacgt gacgccagcc cgccgaagcc cggcactgcg
     3661 ccgtcccccg gtgctccacc gggcgactac ggcaacggcc gcggctcgac ggtcgatggc
     3721 ctgaaggctg ccaacgccgc cttgcgagcc cgtcttgacg aggccgagga gacgatccgc
     3781 gccatccagc agggggcggt ggatgccttc gtcctggagg agttggggcg ccacagggtc
     3841 tacacactgg aaggggggga tcggccctac caggtcttcg tcgagcagat gcagcagggg
     3901 gtcgccacgc tgcacctgga cggcaccgtc atgtacggga atcgccgcct ggcggaaatg
     3961 ctcaagacgc cgcgcgaatc gctcatcggg acatcgctgc gcggtttcgt cgccgagttg
     4021 gacaagcctc tctacgatga tctcctcaag caggggcagg gccgctccgc gcgcggggag
     4081 atccgactgc gcccggcgga gggggaggag tttccggcct atctgaccct gagcctgctg
     4141 ccgggcgagt ccatggtcgg ccttttcgtc accgacctga cggtgcagaa gcaccacgag
     4201 gcgctcgccg cagcgcagga ggcgctgcgc gaggcggacc gccggaagaa cgagttcatc
     4261 gccatgctgg cgcacgagct gcgcaacccg ctggcgccca ttcgcaacgc cgccggcatc
     4321 ttgaagcgga ccgcggacga tccgaagatg gtggaggaga tcggcggcat gctcgagcgc
     4381 cagatcgggc tcatggtgcg cctggtcgac gatctcctgg acgtcagccg catcacccgc
     4441 aaccgcgtcg agttgcgccg ccagcgtctc gagctggcct cggtcctcga gcaggcggtc
     4501 gagtcgatcc ggccgctgtg ccgcaaggcc cggcatcaca tcacggtcgc cctcccgccc
     4561 gagccggtgt acctcgacgc cgacccggtg cgtctcaccc aggtgttcac caatctcctc
     4621 gacaacgcct gcaagtacat ggagccgggc ggccgcgtcc ggctgagcgc cgaggtcacc
     4681 ccggcgcgag acgacgcgcc gggcgaggtc gtggtgcgcg tgcgcgacga ggggatcggc
     4741 atccccgccg aagcgctgtc gcgcatcttc gagatgttcg cgcagatcga cacctcgctc
     4801 gagcgccgca acgccgggct gggaatcggc ttgagcctgg tgaacagcct cgtcgagctg
     4861 cacggtggga aggtcgaggc ccgcagcgag gggaagggtc agggaagcga attcgtagtt
     4921 cgactgccga ccgtcgccga aggttgcgac ggactctcgg cccaagaggg agagaagtcg
     4981 atgaagcgct atcgcatgct ggtggtggac gacaaccgtg attcggccga atcgctcgcg
     5041 ctgctcctga agctcaccgg gcacgacacc cacaccgcgc tggacggaga ggcggccatc
     5101 cgggccgcgg cggagaacca gccggacgtc gtgctgctcg acatcgggat gccgaagctg
     5161 aacggctacg acgcctgcaa gcgcatccgc tcggagccgt ggggacggga catgatcctg
     5221 atcgcccaga ccggctgggg ccaggacgac gaccgccgcc gcaccgagga ggccggtttc
     5281 gacggccacg tcgtcaagcc cgtggacccc gaggatctca tccggatggt ggcccgcctg
     5341 tccaccgccc ggcgccccga acagcagagc tgaagcgccg gcgcgtgccg cgcaaggcct
     5401 gccgcctcag gtcgtcttct ccgcgttgaa cacgcggcgc cagttcctca tccgggcgcc
     5461 actgggtttc ctggtgggcg ccgccgcctt gcgctccgga gcctgcgccg ccgcggaacc
     5521 gacgacgggc gcgacaccgg gagcgccgcc gacgttcggg accggccccg gttccggtcc
     5581 ggccgtcacg ccgcagacgt tcgcggaggc ggagaagctg atgcaggtga cgatgaccgc
     5641 cgcggagcgg gagcaggcgg cctccacctg gcggatgacg ctggcgcagt atctcgagcg
     5701 ccgcagcggg ccgcgccgcg tcccgatcgg gccggaggac gccccggccg cgctgtggaa
     5761 tcccctgctc cccggcctcc cgcgcctgcc ggagacgaat cgtttcgtgc gctcgccggc
     5821 gaccggaggc gcgaagccgc cggcggcgga ggacgacatc gcctacgcgc cggtcacccg
     5881 tctggcgcgc tggatcgaga cgcgcgcgat cacctccgag cggctcaccg gcatctacct
     5941 cgaacgcatc aagcgcttcg acggccggct gcgctcggtc atcacgctga cttcggagcg
     6001 ggcgctcgag cgcgcgcggc aggcggaccg ggagatcgcc gctgggaaat accgcggccc
     6061 cctgcacggc atcccgtacg gcgtgaagga cctcctcgac acgaagggga tccccacgac
     6121 ctacggcgcc gagccgttcc gcgaccgggt gccggccgcg gacgccgcgg tcgtgcgccg
     6181 gctcgacgac gccggggcgg tgctcctggc gaagctctcg ctcggggcgc tcgccctcaa
     6241 cgacatctgg ttcggcgggc agacgatgaa cccgtggctc ccggaggagg gggcctccgg
     6301 ctcgagcgcc ggaccgggcg cggccaccgc ggcggcgctc gtccccttct ccatcggcag
     6361 cgagaccggc gggagcatcg tcagtccggc gatgcgctgc gggctcaccg gcctgcgacc
     6421 caccttcggc cgcgtcgccc gcgccggcgc gatgaccctg tgctggtcgc tcgacaagct
     6481 cggaccgatg acgcgcggcg tcgaggacgc cttcctcgtg ctgcaggcga tcgacggccc
     6541 cgacgccggg gacgtgtcga gcgtcgcgag ccgtctcgac ttcgacgcca ccgcctccgt
     6601 ctccgggctt cgaattggat tcatcccgaa gtggatggac gagcctcccg cgaccgacgt
     6661 cgaccgcgaa gccctccggg tgatgcgcgg gctcggcatg caggtgcgcg aggtgacgct
     6721 tcccgactgg ccgttctcct ccctctaccc gatcctcttc gccgaggggg cggcatcgtt
     6781 cgaggagctg gcgctcgatg gccggctgca cgatctcagg atgcaggtga aggacgcatg
     6841 gccgaacctc ttccgccagg cgcgcttcct gtcggcggtc gatctcgtgc aggccgaccg
     6901 gctgcggcgt aaggtttcaa tgctgatggc cgggatcttc cgcgacctgg acctgcttct
     6961 ggtgccgtcg ctgcgggacg agcagctcac catcaccaac ttcaccggcc atccttcgct
     7021 gacgctgcgc gccggcttcg tgaaggtctc cgaggcgcgc agcgactggg cccccgaccc
     7081 cgagcgcccg ctgccgaagt tcgacccacc ccgccgcgtg ccgcacggca tcaccctcat
     7141 cgggcggctg ttcgacgacg gcctcctcgg ccgcgccggc atcgccctcg agaaggcctt
     7201 cggggtcgcc gcggagaaac ccccgcgctt ctgaggggat gcgggtcagc ggaagcgctc
     7261 tcgttcccgc cccgcatggc cttgacggcc gacggctctt cggtcatcgt ccgccggcgc
     7321 cgccgcgtcc gctgaaaccc ggcgcgccgg gacggggcat gacccttcac ctgaacagat
     7381 gaatggctga gccgcggccg caggccgagg ccgcgctcgt ccgcgggctc gggacgctcg
     7441 atgtcgggct gatcacgttc aacgccctcg tcggctcggc gatcttcatc gccgccgcca
     7501 tcgtgccccg ggcggcgccg catgcctggc tcatcctgcc gctgtggatc gccggcgggg
     7561 ccctctgtct cgcgggggtg accacctacg ccgagctcgg cacgatgttc ccgaaagcgg
     7621 gcgggcagta ccagtacctt aaagaagcct atggcccgct gtgggggttc ctcttcggat
     7681 ggaccgcctt cctcgtcatc cagtgcggcg ccatcgccta catcagcgtc gccggggcgg
     7741 acgggctcgg gcgcatctgg ccggcgctcg gctcgtcgca cacgctgctc acgattccac
     7801 tcggatcggc gacctggcgc gtccagggaa gccaggccgg cggggcggcg ctgatcgccg
     7861 gcatcacgtt cgtcaactac ttcgggctgc gcgccggcgc cgggctgcag aacgccatcg
     7921 gcgcggtgaa gatcgtgtcg ctcctcgtct tcatcggctt cggcctggca gtgacgccga
     7981 ccgctccgga tccgtggtcg gagccgctcc cgcaggggtc gctcctgacg ccgctcgcgg
     8041 tcgccatgat cggcgtgctc tggtgctacg acggcttcta ccaggcgagc ttcagcgctg
     8101 gcgagatccg cgacccggcg aagagcctgc cgcgcgggat gatcctcggg accgccggca
     8161 ccgtggtcct ctacgcgctc gtgaacatcg tctacctgcg cgccctcccg cccaaagcac
     8221 tcggggaggc gccgcgggtc gccgaagcgg cggcggccgc cctgttcggc gaggggcccg
     8281 ggcgcttcat gtcggcggcg gtcctcgtgt cgatcctcgg gtgcctcgcg acctgcatat
     8341 tgaccgccgc gcgcatctac ctcccgatgg cgcaggacgg gttgttcttc cgatcgctcg
     8401 cccgcgtcca tccggtctac cggacgccgg gagcgtgcct cgtcgcgcag gccgcctggt
     8461 cgagcgtgct cgccttcagc ggctcgtacg agcagctcgg c
//
LOCUS       DASRIQ010000171         9524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3190000, whole genome shotgun sequence.
ACCESSION   DASRIQ010000171 DASRIQ010000000
VERSION     DASRIQ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3190000"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFD06_04175"
     CDS             <1..714
                     /locus_tag="VFD06_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN02767.1"
                     /translation="SETMARRFWPGQDPIGRRFHPFNANNPPVTVVGVAGDVRLFTLD
                     ETPGPAVYLFFVRWNPIQIAVRTQGEPGAFAGLLRDEVRAIDPGVPVAQIRPMEELLG
                     GTTSPRRFTMTLLALFAVAALLLSGVGLFGLMAFLVAQRTRDIGVRLALGARRRDILG
                     LVVGRAMLLTSIGVAAGLAGALALAGAIRSMLYGVGARDPLTLAATALLLGLVALLAC
                     WIPARRAARIDPMVALRCE"
     gene            complement(745..1449)
                     /gene="mtgA"
                     /locus_tag="VFD06_04180"
     CDS             complement(745..1449)
                     /gene="mtgA"
                     /locus_tag="VFD06_04180"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HZN02768.1"
                     /translation="MMKGTRRRRLAIWLAAGIGLALAFWHLWTFGQVWRLRWNNPRTT
                     AFMEAGLERLRARDPHARLQHRWVPYGRVSDHLKRAVLAAEDQRFLEHDGFDVGAIER
                     AYETNTRRGRIRHGGSTISQQLAKNLFLSPHRSYFRKGREAVITLMIESVLGKRRILE
                     LYLNVIEWGDGVYGAEAAARYYFGVAAADLGPDESALLAAVIPKARVYSHLPVTPYLE
                     ERQAALLRQMESVRIP"
     gene            complement(1446..3236)
                     /locus_tag="VFD06_04185"
     CDS             complement(1446..3236)
                     /locus_tag="VFD06_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02769.1"
                     /translation="MSDAGPLALVPIVFGLSAFGPGYRLVRRAGWPPLETAVAGLALS
                     VLLVGQASFVIGLLWLPAVWRVLLLVACAAATIASVPEVVRLVRSDAGARTALVWFAI
                     LAVWGVGLQACVRNYSGGNIVYDWLDHYERSKFFLGFQPTDKLFSGALLPARPPLMNA
                     FAAQILALTRPRFPCYQMTAALLGLLAYFPMLLVARLFSASRGLPRILAVVLMFQPMF
                     LQNVSYSWTRMTAVFFTLSAIGFYVSGWRAGDGRRTLMAFACGAGAILTHYSSAPYVL
                     FLALHFLLFALPRRPRPFRELAAIVVAATLVLLPWLAWASAVYGRETVVSAGLRSSGK
                     PLPTFGANVLKAALNLRDTFVPPLLRDVPHDPEQQSWGWGRIRDEAFLMYQGNLPLAL
                     GSLAALFLATEALRAWRRRRAAERAGAGRASEPERGPPADSWRFWVSLAAFVILTGIG
                     VQGGRDPFGLAHTAQQPLVMIGVSFLAARFGTWLRWQRLLAAAGLLLDALLGVLLQFW
                     LQSYSLDLPSNWRGPLGLTFKDLTRMMATWNWGEKVSGRHLFVADAIGGIGWVLLPVL
                     ALLFAVAAATLLRQASRRATSSPHATTNPG"
     gene            complement(3347..3976)
                     /locus_tag="VFD06_04190"
     CDS             complement(3347..3976)
                     /locus_tag="VFD06_04190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HZN02770.1"
                     /translation="MNRATAMKKNAVLLGSLAVTLLLATSGAAAYCLIGVRWPSTGNI
                     RYNPNGKVTSGQCISASQMDSAVTGGITPWKPLRYLGTTSNTANKRDGMNVVGWANLG
                     GQTLGVTNFLRYDRQATVSCNGNLFSNLIESDVRITTTYRWTSGGGQCPCAAGSAFYL
                     NGVSEHEFGHVTGLCHTNNPNSLMYPSFGVCENKSSGSDENAGENALCY"
     gene            complement(3973..4554)
                     /locus_tag="VFD06_04195"
     CDS             complement(3973..4554)
                     /locus_tag="VFD06_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02771.1"
                     /translation="MTRRILTGCVLALAAGAAMASTVIGLSVEDQARLSTMVVVGEVL
                     HQRGVNHPEHGIETAVTLKVTEVLKGDVRTKQAITFHTRGGEVDGEISQAMGEAIFKP
                     GETVLVFIESVDGRLYNLGLSMGVWKVHENAIGDVTFTRDIQDGLSIVGEAAVQHGPL
                     PYAEMVSRVRYAARNPRVDNDMVRGRLIEGRER"
     gene            complement(4663..5472)
                     /locus_tag="VFD06_04200"
     CDS             complement(4663..5472)
                     /locus_tag="VFD06_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02772.1"
                     /translation="MRSSWTAFANRVLPIAAAALLAACGGRDAAAPEAPAASAPAAPG
                     PRYVCEYDPDPANLPECELESKYLADPIESSMMMMLEVTRFPAIDPTPEQKKAAEDLV
                     RRCHASAEQHGWHDFAKATADGYKLKVSDDTHYNNVEFLFDDRVLDPDRPEYLMFYDT
                     PKGKVLTGFMFVPRSNTEEGPQIGGNLTRWHYHTWHKPICMVKGIYDTGPVVDGTCAT
                     GSPSARSPEMLHVWLLDHPQGPFATAMGLPPALLERLLAEREARSAASATK"
     gene            5634..6980
                     /locus_tag="VFD06_04205"
     CDS             5634..6980
                     /locus_tag="VFD06_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZN02773.1"
                     /translation="MPDHERRVYESILEMLSSEENPTPLVRLRKVVPYQHTRVYAKLE
                     WYNPFGAVKDRIAANLLQDAETRGTLKPGEKSLVEPTSGNTGLGLAMMANARGYALRT
                     PLSKKIPLEKRAVLRFFGADVVELDDELCPAPGAPEGAIAYARQTARMPDFHMLDQYR
                     NEANPESHFKTTGPEIWRQTQHQVTHFAAGLGTCGTITGTGRFLKTQNPAVKVLGIHP
                     QEGHDIPGVRSLKQLVQTELFAPKEYDQTIDVANEEAYALCLRLVREEQIIAGPSSGM
                     ALAGALKMVPDAPGNLLVVIFPDNIFKYASSVMRHFPAMFPDAAKAASPASELNARLF
                     DEMVAHAKTSPDALPVDAAHTLLEKGGATVIDVRSAEKYEEGHVPGAINIPLATLGAS
                     LDRLPARKDQPILTVCDRGNISANGMLYLKSLGYSNVKSLSGGTNAWKQEGHPTEP"
     gene            7018..8064
                     /locus_tag="VFD06_04210"
     CDS             7018..8064
                     /locus_tag="VFD06_04210"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN02774.1"
                     /translation="MRPISAARRPTTLAATALLGAIGAGVSWSQAPAPSPAPPAEPPP
                     VAAAPAAPATPAVPAPPAPPPGPDRGIRNKVAAGDLLSAESILEEHRVEKGEDSAWVN
                     GLGWLARGALLLGDLDKAARYAAQTRSRCADAVARGAKLEEDHPLEIALGAAIEVEAQ
                     LVERKAGARKAAEQLRKELSGLSGPVALRSRIQKRINILTLAGTPAPALVAEDFLGDP
                     PPSLAALRGKPIVLFLFAEWCGDCKAQQRALAAAWKRRAGDGTALIALTRYYDEEAKR
                     SAEKDRVAVVWKTSYPDVGAVPIVISTASMERYGGSSTPSFVFIDRAGIVRGYAPTRL
                     TEAELDARLQALAR"
     gene            complement(8101..9465)
                     /locus_tag="VFD06_04215"
     CDS             complement(8101..9465)
                     /locus_tag="VFD06_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN02775.1"
                     /translation="MSAPDPSPIDDVVSEPTARPRGLSRVIGAAAAGTVIEWYDFYIF
                     GSMAGILAEHFYPAGNPTAAFLSTLATFATGFVVRPFGAVVFGRIGDLVGRKYAFLVT
                     LLLMGLGTAAIGLIPGYATIGVAAPVLLVLMRLLQGLALGGEYGGAAIYVAEHAPDAR
                     RGFYTSFIQITATAGLFVSLGVIEATRTALGVDAFRAWGWRVPFLLSILLVGLSFYIR
                     VSLKESPLFSRLKAQGKTSMRPIRDTLASRENRRLMLLALFGATAGQGVVWYTGQFYA
                     LIYLQKVLHVGDRASNVIVAAALLCAVPFFVVFGALSDRIGRRPIILAGCLLAALSYV
                     PIYAAMSAVSSPPRMVALTALVFVQVLFVTMVYGPIAAFLVEFFPARIRYTSLSLPYH
                     LGNGVFGGLTPFVATAIATAAGNPYAGLAYPIAIAAMTFVIGVFFLPETRRRSIWAEV
                     DGAE"
BASE COUNT         1539 a         3332 c         3354 g         1299 t
ORIGIN      
        1 agcgagacga tggcgcggcg cttctggccc ggccaggatc cgatcggacg acgtttccat
       61 ccctttaacg cgaacaaccc tccggtcacg gtcgtcggcg tagcggggga cgtccggctc
      121 ttcaccctcg acgagacgcc gggacctgcc gtctacctct tcttcgtgag gtggaacccg
      181 atccagatcg ccgtgcgcac gcagggggag ccgggcgcct tcgccggcct gctgcgcgac
      241 gaggtgcggg cgatcgatcc cggcgtgccg gtggcgcaga tccggcccat ggaggagctg
      301 ctcggcggca ccacctcccc gcgacggttc accatgacgc tcctggcgct cttcgcggtc
      361 gcggcgctgc tcctgtcggg tgtcgggctg ttcggtctca tggcgttcct ggtggcacag
      421 cggacgcgtg acatcggtgt ccgcctggcc ctcggggcga ggcggcgcga tatcttaggg
      481 ctggtggtgg gacgcgccat gctcctgacc tcgatcgggg tcgccgccgg cctcgctggc
      541 gctctggcgc tcgccggagc gatccgcagc atgctctacg gggtcggcgc gcgcgatcca
      601 ctgaccctgg ccgcgaccgc gctgctgctc gggctggtgg ccctcctggc gtgctggatc
      661 ccggcgcggc gggcggcgcg gatcgacccg atggtcgcgc tgcgctgcga gtgacttcta
      721 ccggcgcgac gaggctcctc gccgtcaggg aatgcggacg gactccatct ggcggaggag
      781 ggccgcctgg cgctcctcga ggtacggcgt caccgggagg tgcgagtaga cgcgcgcctt
      841 cgggatcacc gccgccagaa gcgccgactc gtccgggccg aggtcggccg ccgccacgcc
      901 gaagtagtac cgggccgccg cctcggcgcc gtagacgccg tccccccact cgatcacgtt
      961 gagatacagc tccaggatgc ggcgcttgcc caggacgctc tcgatcatca gggtgatcac
     1021 cgcttcgcgc cccttgcgga agtatgagcg gtgcggcgac aggaacaggt tcttggcgag
     1081 ctgctggctg atcgtcgagc cgccgtggcg gatccgcccg cggcgcgtgt tggtctcgta
     1141 cgcccgttcg atcgctccca cgtcgaagcc gtcgtgctcg aggaagcgct gatcctcggc
     1201 ggcgaggacc gcgcgcttca ggtggtcgga gacgcggccg tacggcaccc atcggtgctg
     1261 caggcgcgcg tgcgggtcgc gggcgcgcag gcgttcgagc cccgcctcca tgaacgcggt
     1321 cgtccgcggg ttgttccagc gcagccgcca cacctgcccg aaggtccaga gatgccagaa
     1381 cgccagcgcc aggccgatgc cggcggccag ccagatcgcc aggcgacggc ggcgcgtccc
     1441 tttcatcatc ccgggttcgt ggtcgcgtgc ggcgatgacg tcgcccgtcg cgacgcctgc
     1501 cggagaagcg tggcggcggc gacggcgaac agcagcgcga ggaccggaag cagcacccag
     1561 ccgatccctc cgatcgcatc cgcgacgaac aggtgccgcc cgctgacttt ctccccccag
     1621 ttccaggtcg ccatcatccg ggtgaggtct ttgaaggtga gaccgagggg gccgcgccag
     1681 ttggacggca ggtccagcga ataggactga agccagaact gcagcagcac gccgagcagg
     1741 gcgtcgagga gaaggccggc cgccgcgagc aggcgctgcc agcgcagcca cgtcccgaac
     1801 cgggcggcga ggaacgacac gccgatcatc accagcggct gctgcgccgt gtgggccaag
     1861 ccgaacggat cacgtccccc ctgcacgccg atgccggtga ggatcacgaa cgcggcgagc
     1921 gagacccaga accgccagga atcggcaggc ggtccgcgtt ccggctccga cgcgcggccc
     1981 gcgccggccc gttctgccgc acgacggcgt ctccacgcgc gcagcgcttc ggtcgcgagg
     2041 aacagcgccg cgagcgagcc gagcgccagc ggcaggttcc cctggtacat caggaacgcc
     2101 tcgtcccgga tccgccccca gccccaggac tgctgctccg gatcgtgggg cacgtcgcgg
     2161 agaagcggcg ggacgaaggt gtcgcgcaaa ttcagtgcgg ccttgaggac attcgcaccg
     2221 aaggtcggga gcggcttgcc ggacgagcgc agaccggccg agacgaccgt ctcgcggccg
     2281 tacaccgccg acgcccaggc gagccatggc aggagcacca gcgtcgcggc gacgacgatg
     2341 gcggcgagct cccggaacgg ccgcgggcgt cgggggagcg cgaacaggag gaagtgcagc
     2401 gccaggaaca gcacgtacgg agccgacgaa tagtgcgtga ggatcgcccc cgcgccgcag
     2461 gcgaacgcca tgagcgtccg ccggccgtcg ccggcccgcc agccgctgac gtagaacccg
     2521 atcgccgaca gcgtgaagaa aaccgcagtc atccgggtcc aggaatacga cacgttctgc
     2581 aggaacatcg gctggaacat cagcacgacg gcgaggatgc gcggcaaccc gcgggacgcc
     2641 gagaacagcc gcgcgacgag cagcatcggg aaatacgcca gcaggcccag cagcgccgcg
     2701 gtcatctggt agcaggggaa gcgcggccgc gtcagcgcga ggatctgcgc cgcgaaggcg
     2761 ttcatcagcg gcggccgcgc cggcagcagc gccccggaga acagcttgtc ggtcggctgg
     2821 aacccgagga agaacttgct gcgctcgtag tggtcgagcc agtcgtagac gatgttcccc
     2881 ccggaatagt tccggacgca ggcctggagc cccacccccc agacggccag aatcgcgaac
     2941 cagacgaggg cggtccgcgc tcccgcgtcg gagcgcacga gtcgcaccac ttccggcacg
     3001 gaggcgatgg tcgcggcggc gcacgcgacg agcagcagca cgcgccagac cgccgggagc
     3061 cacagcaggc cgatgacgaa ggaggcctgg ccgaccagga ggacggagag ggccaggccc
     3121 gcgaccgcgg tctcgagcgg cggccagccg gcgcgccgga cgagcctgta cccgggcccg
     3181 aacgccgaca gcccgaagac gatgggaacg agcgcgagcg gccccgcgtc agacatcccg
     3241 gctcacggac acgggcgcct cgaagacctc gaaggggaag gggcgtcagg ggcgcgccgc
     3301 gcggcgccgg atcggccggc gccgcgcggc gggcctcaaa gggaactcag tagcagaggg
     3361 cgttctcgcc cgcgttctcg tcgctgccgc tgctcttgtt ctcgcagacg ccgaacgacg
     3421 ggtacatgag cgagttcggg ttgttcgtgt ggcagagccc cgtcacgtgg ccgaactcgt
     3481 gctccgagac gccgttcagg tagaaggcgg agccggcggc gcaggggcac tggcctccac
     3541 cggacgtcca gcggtaggtc gtggtgatcc gcacgtccga ttcgatgagg ttcgagaaca
     3601 ggttgccgtt gcaggagacc gtcgcctgcc ggtcgtagcg caggaagttg gtgaccccga
     3661 gcgtctgccc gccgaggttc gcccagccga cgacgttcat cccatcgcgc ttgttcgcgg
     3721 tgttgctcgt cgtcccgaga tagcgcagcg gcttccacgg cgtgatgccg ccggtgaccg
     3781 cgctgtccat ctgcgaggcg ctgatgcact ggccgctggt caccttgccg ttcgggttgt
     3841 agcggatgtt gccggtcgag ggccagcgaa cgccgatgag gcagtaggcc gccgcgcccg
     3901 aggtcgcgag cagcagcgtc acggcgaggc ttccgaggag gacggcgttc ttcttcatcg
     3961 cggttgccct gttcatcgct ccctcccctc gatcagccgg ccgcggacca tgtcgttgtc
     4021 gacgcgcggg ttgcgggcag cgtaacgcac gcgcgacacc atctcggcgt agggcagggg
     4081 accgtgctgc accgccgcct cgccgacgat cgacagaccg tcctggatgt cccgcgtgaa
     4141 ggtgacgtcg ccgatcgcgt tctcgtgcac cttccagacc cccatcgaga ggcccaggtt
     4201 gtacagccgg ccgtccaccg actcgatgaa gacgagcacg gtttcgcccg gcttgaagat
     4261 cgcctccccc atcgcctggg agatctcgcc gtccacctcg ccgccgcgcg tgtggaacgt
     4321 gatcgcctgc ttcgtcctca cgtccccttt gagcacctcg gtgaccttga gggtcaccgc
     4381 ggtctcgatg ccgtgctcgg gatggttcac cccccgctgg tgcagcacct cccccacgac
     4441 gaccatcgtg gacagccgcg cctggtcttc cacggacagt ccgatgaccg tgctcgccat
     4501 cgcggctccc gccgccaggg cgaggacgca ccccgtcagg attcttcgcg tcatgcgtgt
     4561 ccctccgaag aaagtgaaga gcgaacagcg tgttgcggcc ttataacccc gaggcgcgcg
     4621 cgagggcaag gcgggcccat cgccccggcg gtctgatgcg cgttacttcg tcgcggacgc
     4681 ggccgaccgc gcctcgcgct cggcgagcag ccgctcgagg agagcggggg gcaggcccat
     4741 cgcggtggcg aacggacctt gcggatggtc gagcagccag acgtgcagca tctcagggct
     4801 gcgggccgac ggggagccgg tcgcgcacgt gccgtccacc accgggccgg tgtcgtagat
     4861 ccccttcacc atgcagatcg gtttgtgcca cgtgtggtag tgccagcggg tcaggttgcc
     4921 gccgatctgc ggcccctcct cggtgttgct gcgcggcacg aacatgaacc cggtgagcac
     4981 cttccctttc ggcgtgtcgt agaacatcag gtattcgggg cggtcgggat cgaggacgcg
     5041 gtcgtcgaac aggaactcga cgttgttgta gtgcgtgtcg tccgaaacct tcagcttgta
     5101 cccgtcggcc gtcgccttgg cgaagtcgtg ccagccgtgc tgttcggcgc tcgcgtggca
     5161 gcggcgcacg agatcctcgg ccgccttctt ctgctcgggg gtcggatcga tcgccgggaa
     5221 gcgcgtcacc tccagcatca tcatcatgct cgactcgatc ggatcggcga ggtacttgga
     5281 ctcgagctcg cactccggga ggttggccgg gtccgggtcg tactcacaga catagcgcgg
     5341 acccggcgcg gcgggcgcgg aggcggcggg agcctcgggc gcggcggcgt cacgtccgcc
     5401 gcacgcggcg agcagcgccg ccgcggcgat cggcaggacg cggttggcga aggctgtcca
     5461 ggaggatcgc atcgcggcgg gatgctatct caggcctcct cgagaggcca gacctcgagg
     5521 agcgccgcca ggtcgggggg acgtctttcg gccatgggca tccgttccgg aaggtgaccc
     5581 atcgtagcgg acgtttccgg atgggaccgc agggatctat actttggcca cccatgcccg
     5641 accacgaacg ccgcgtctac gagtcgatcc tcgagatgct ttcgagcgag gagaatccca
     5701 cccccctcgt ccgtctgcgc aaggtcgtcc cgtaccagca cacgcgggtc tacgccaagc
     5761 tcgagtggta caacccgttc ggcgccgtca aggatcgcat cgccgcgaac ctcctgcagg
     5821 atgccgagac acgcggcacc ctgaagcccg gggagaagtc gctggtggag ccgacctccg
     5881 gcaacaccgg gctcgggctc gcgatgatgg cgaacgcgcg gggctacgcg ctgcggaccc
     5941 ccctgtcgaa gaagatcccg ctcgagaagc gggcggtgct gcgcttcttc ggggcggacg
     6001 tcgtcgagct ggacgacgag ctgtgcccgg cgccgggggc cccggagggg gcgatcgcct
     6061 acgcgcggca gacggcgcgc atgcccgact tccacatgct cgaccagtac cgcaacgagg
     6121 cgaaccccga gagccacttc aagaccaccg gccccgagat ctggcggcag acgcagcacc
     6181 aggtgacgca cttcgcggcg gggctcggca cctgcggcac catcaccggc accggccgct
     6241 tcctcaagac gcagaacccg gcggtgaagg tgctcggcat ccacccgcag gaagggcacg
     6301 acatccccgg ggtccgcagc ctcaagcagc tggtgcagac cgagctgttc gcgccgaagg
     6361 agtacgacca gacgatcgac gtcgcgaacg aggaggccta cgcgctctgc ctgaggctcg
     6421 tgcgcgagga gcagatcatc gcgggcccct cgtccggcat ggctctggcg ggggcgctca
     6481 agatggtgcc ggacgcgccg gggaacctgc tcgtcgtgat ctttccggac aacatcttca
     6541 agtacgcctc gtcggtcatg cgacatttcc cggcgatgtt ccccgatgcc gcgaaggcgg
     6601 cctcccccgc ctcggagctg aacgcccgac tcttcgacga gatggtggcg cacgcgaaga
     6661 cctccccgga cgccctgccc gtggatgcgg cgcacaccct gctcgagaaa gggggagcga
     6721 cggtcatcga cgtacgctcc gccgagaagt acgaggaggg gcacgtgccg ggggcgatca
     6781 acatcccgct cgcgacgctc ggcgcctccc tcgatcgcct gccggcccgc aaggaccagc
     6841 cgatcctcac cgtgtgcgat cgcggcaaca tctccgccaa cggcatgctc tacctcaagt
     6901 cgctcggcta ctcgaacgtc aagagcctga gcggcggcac caacgcctgg aagcaggaag
     6961 gtcaccccac cgaaccctag gcctctgatg ccggcgcggc ccgccgggga gactccgatg
     7021 cgcccgatct ccgctgctcg aagaccgacc acccttgccg cgaccgcgtt gcttggcgcg
     7081 atcggagccg gcgtttcgtg gagccaggcc cctgcacctt cacctgcgcc cccggccgag
     7141 ccccctccgg tcgccgcagc accggccgcc cccgcgacac cggccgtccc ggcgccgccg
     7201 gcgccgccgc ccggcccgga tcgcggcatc cgcaacaagg tcgcggctgg tgatctcctg
     7261 agcgccgagt cgattctcga ggagcatcgc gtcgagaaag gcgaggattc cgcctgggtg
     7321 aacggcctcg gctggctggc gcgcggcgcc ttgctgctgg gcgatctgga caaggcggcg
     7381 cgctacgccg cgcagacgcg gtcgcgctgc gccgacgccg tcgcccgggg ggcgaagctc
     7441 gaggaggatc acccgctgga gatcgccctc ggcgccgcga tcgaggtcga agcccagctc
     7501 gtcgagcgga aggccggggc gcgcaaggcg gcggagcagc tccggaagga gctgtccggt
     7561 ctctccggac cggtggcgct gcgctcgcgg atccagaagc ggatcaacat cctgacgctg
     7621 gccggcaccc cggctccggc cctcgtggcc gaggacttcc tcggcgaccc gccgccgagc
     7681 ctcgccgcgc tccggggcaa gccaatcgtc ctgttcctct tcgccgagtg gtgcggcgac
     7741 tgcaaggcgc agcagcgggc gctcgcggcg gcgtggaagc gccgagccgg ggatggcacg
     7801 gcgctcatcg ccctgacgcg ctattacgac gaagaagcga agcgctccgc cgagaaggat
     7861 cgggtcgccg tggtctggaa gacgtcctac cccgacgtcg gcgccgtccc gatcgtgatc
     7921 agcaccgcct cgatggagcg ttacggcggc tcgagcacgc cgtccttcgt gttcatcgac
     7981 cgggcgggaa tcgtgcgcgg ctacgcgccg acgcgcctca ccgaggccga gctcgacgcc
     8041 aggctgcagg ccctcgcccg ctgatcgaaa agccgcgcgc ctggcccgcg cccggcgccg
     8101 tcattccgcc ccatccacct cggcccagat cgatcgccgc cgcgtctcgg ggaggaagaa
     8161 gaccccgatc acgaaggtca tcgccgcgat ggcgatcggg tacgcgaggc cggcgtacgg
     8221 gttgccggct gcggtggcga tcgccgtggc gacgaacggc gtgagccccc cgaagacgcc
     8281 gttccccagg tggtatggga gcgacagcga ggtgtagcgg atacgggccg ggaagaactc
     8341 cacgaggaac gccgcgatcg gtccgtacac catcgtcacg aagaggacct gcacgaacac
     8401 cagcgccgtc agcgccacca tccggggcgg cgacgacacg gcgctcatcg cggcgtagat
     8461 cggcacgtaa ctcagcgctg cgagcaggca ccccgccagg atgatcgggc gccgcccgat
     8521 ccggtcggag agggcgccga agacgacgaa gaacggcacg gcgcacagga gcgccgccgc
     8581 cacgatgacg ttcgacgccc gatcccccac gtgcaggacc ttctgaagat agatgagggc
     8641 gtagaactgc cctgtgtacc agaccacccc ctgcccggcc gtggcgccga aaagcgccag
     8701 gagcatcaga cggcggttct cgcgactcgc gagggtgtcg cggatcgggc gcatggaggt
     8761 cttcccctgc gccttcagcc gcgagaacag cggcgactcc ttcaggctga cgcggatgta
     8821 gaaggacagc ccgacgagga ggatggagag caggaagggc acgcgccagc cccaggcccg
     8881 gaaggcgtcg acgccgagcg ccgtgcgcgt cgcctcgatc acgccgagcg agacgaacag
     8941 cccggcggtg gcggtgatct ggatgaagct cgtgtagaag ccccgccgcg cgtcgggggc
     9001 gtgctcggcg acgtagatcg ccgcgccgcc gtactcgccg ccgagcgcca gcccctgcag
     9061 gagccgcatc aggaccagga gcaccggcgc cgccacgccg atcgtcgcgt agcccgggat
     9121 gagcccgatc gccgccgtcc ccagtcccat cagcagcagg gtcacgagga aggcgtactt
     9181 gcggccgacg agatcgccga tgcggccgaa gaccaccgcg ccgaaggggc ggacgacgaa
     9241 gccggtcgcg aacgtcgcca gcgtggacag gaacgccgcc gtgggattcc ccgcggggta
     9301 gaagtgctcg gcgaggatcc ccgccatgct cccgaagatg tagaagtcgt accactcgat
     9361 caccgtcccg gccgcggccg cgccgatgac ccgggacagg ccgcgcggcc gagcggtggg
     9421 ctcgctcacg acgtcgtcga tcggagaggg atccggagcg ctcatggggg cgcatcatag
     9481 cgaaccgtcg cgccggcgcc acgccgtcga ccgggagaaa gaga
//
LOCUS       DASRIQ010000172         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_841472, whole genome shotgun sequence.
ACCESSION   DASRIQ010000172 DASRIQ010000000
VERSION     DASRIQ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841472"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VFD06_04220"
     CDS             complement(<1..1420)
                     /locus_tag="VFD06_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZN02776.1"
                     /translation="MNKEMIISSGDHDTRVAILEDDQVVEIFIERERSRGVVGNIYKG
                     RVSKVLPGMQSSFIDVGLERDAFLYVSEVVNTVEEFDRLEAGDDDEDLTPAPVSEPVA
                     VADGVAVEELSAPAQNGGARVERRSRDRDQSRGPREDRPEPKIEDLLKEGQEILVQVV
                     KEPLGTKGARLTSHVTMPGRFLVFMPTVDHVGVSRKIESREERARLRGIVRTFREAHG
                     FTGGVIIRTAASGRSDEDIVSDLSWFHQIWTEIRQKMDGRRAPAVLFQEQSLVVKLLR
                     DLLTDDYTAIRIDDDQEHRRVLALIERIMPGLQPRVKLYTKTFPIFEEYGVQGEIDKA
                     LRSKVWLKSGGYLVINQTEALVAIDVNTGRYVGKKSSGRLEDTIVKTNLEAVKEIVRQ
                     LRLRDLGGIIVLDLIDMEEKKNRQRVFQEVEKELRKDRSPSKALQVSDFGLVIVTRKR
                     VKQSLERVLTEPCPYCSGAGTIK"
     gene            complement(1612..2640)
                     /gene="rodA"
                     /locus_tag="VFD06_04225"
     CDS             complement(1612..2640)
                     /gene="rodA"
                     /locus_tag="VFD06_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZN02777.1"
                     /translation="MIYSTTYVRLPDGSGHAGREFWTQLYALALGCVALLACLTIDYR
                     LLAEHSLFLYAGLLGLLVFVLMAGDSAMGAQRWIELGRFKFQPSEFARMTLALILAMF
                     FGENRRGARNTSDLVIGGIFVVVLFLLIVKQPDLGSAMTLLPVFLGVAYLAGLRLRLL
                     AIAAAIAILLTPIAWKYGLKDYQKSRIVIFIDPEQDPRGAGYQPIQARVSVGSGGLRG
                     KGFMEGTQGQYKFLPVAHNDFIFSVLAEEFGFLGVLAALGLYLFVILRSLEAARLAKD
                     RIGAYLVGGIISGFTFQVIYNITMSAGLAPVKGLTLPLMSYGGSSIIATLAAFGLILN
                     VRMRRFTN"
BASE COUNT          478 a          921 c          901 g          443 t
ORIGIN      
        1 acttgatcgt gccggcgcct gaacagtagg gacagggctc ggtgagcacc cgctccaggc
       61 tctgcttgac acgcttgcgg gtcacgatca ccagcccgaa gtcggacacc tgcagcgcct
      121 tcgacggcga ccgatccttc cgcagctcct tctcgacctc ctggaagacc cgctgccggt
      181 tcttcttctc ctccatgtcg atgagatcga gcacgatgat gccgcccaga tcgcggaggc
      241 ggagctggcg cacgatctcc ttcaccgcct ccaggttggt cttgacgatc gtgtcctcga
      301 gccggccgct cgacttcttg ccgacgtagc ggccggtgtt cacgtcgatg gcgaccagcg
      361 cctcggtctg gttgatcacc aggtagccgc ccgacttcag ccagaccttg ctgcggaggg
      421 ccttgtcgat ctccccctgg acgccgtact cctcgaagat cgggaacgtc ttcgtgtaca
      481 gcttcacccg gggctgcagc cccggcatga tgcgttcgat cagcgcgagc acccgccggt
      541 gctcctggtc gtcgtcgatg cggatggcgg tgtagtcgtc ggtcaggaga tcgcgcagca
      601 gcttcaccac caggctctgc tcctggaaga gcacggcggg cgcgcggcgg ccgtccattt
      661 tctgccggat ctcggtccag atctggtgga accaggagag gtcgctgacg atgtcctcgt
      721 cggagcggcc ggatgcggcg gtgcggatga tgacgccgcc ggtgaacccg tgggcctcgc
      781 ggaacgtccg cacgatgccg cgcagccgcg cgcgctcttc ccgcgactcg atcttcctcg
      841 agacgccgac gtgatcgacc gtcggcatga acaccaggaa gcgtccgggc atggtgacgt
      901 gggaggtgag gcgcgcgccc ttggtgccga gcggctcctt gacgacctgg accaggatct
      961 cctgcccttc cttgagcagg tcctcgatct tcggctcggg ccggtcctcg cggggccccc
     1021 gggactgatc gcgatcgcgg ctgcgccgct cgacgcgcgc gcccccgttc tgcgccggag
     1081 cggaaagctc ttcgaccgca acgccgtcgg ccacggccac cggctcgctc accggagccg
     1141 gggtcaggtc ctcgtcgtcg tcgcccgctt cgaggcggtc gaactcttcg acggtgttga
     1201 cgacttcgga cacgtagagg aacgcgtccc gctcgaggcc gacgtcaatg aaggaggact
     1261 gcatcccggg gaggaccttg gagacccgcc ccttgtagat gttgccgacc acgccgcgcg
     1321 atcgctcgcg ctcgatgaag atttcgacga cctgatcgtc ttcgaggatg gcgacgcgcg
     1381 tatcgtggtc gccagaggag atgatcatct ccttgttcat ggatgaagct ccgctgatct
     1441 gcggacgcag gcgcgacccc ggcgcgcacg gacgcggccc gtcgcgcggg ccgaggcccg
     1501 gcggcgaccc gcaccgtcgc gatgatgctc agcatgagca cgagcaagct gccggtgaag
     1561 accatctgcc tgcgatgtcc ggaaaaggtg gacctgaagc cggcttcagg cctaattcgt
     1621 gaaccgccgc atcctgacat tcaggatgag gccgaaagcc gccagcgtcg caatgatcga
     1681 cgacccgccg taactcatca gcggaagcgt cagccccttg acaggtgcga ggccggccga
     1741 catggtgatg ttatagatca cctggaacgt aaatcccgaa atgatccctc cgaccaggta
     1801 ggcgccaatg cggtccttgg cgagccgcgc cgcctcgagc gacctgagaa tcacgaacag
     1861 atagagacca agggcggcaa ggacgcccag gaacccgaac tcctccgcga gcacggagaa
     1921 gatgaagtcg ttgtgggcga ccggcaggaa cttgtactga ccctgcgtgc cctccatgaa
     1981 gcccttgccg cgcaggccgc cggagccgac gctgacgcgg gcctggatcg gctgataccc
     2041 ggcgccccgc ggatcctgct ccgggtcgat gaatatcacg atgcgcgatt tctggtagtc
     2101 cttgagcccg tatttccagg cgatcggcgt gagcaggatg gcgatcgccg cggcaatcgc
     2161 cagcagccgc agccgcagcc cggccagata ggcgaccccg aggaagaccg gcagcagcgt
     2221 catcgcggag ccgagatccg gctgcttgac gatcagcagg aacagcacga cgacgaagat
     2281 gccgccgatg acgagatcgg acgtgttgcg cgcgccgcgc cggttctcgc cgaagaacat
     2341 cgcgaggatc agcgccagcg tcatgcgggc gaactccgat ggctgaaact tgaagcggcc
     2401 gagctcgatc cagcgctggg cgcccatcgc gctgtcgccg gccatgagca cgaacaccag
     2461 caacccgagc aggccggcgt acaggaacag ggagtgctcc gcgagcaggc ggtagtcgat
     2521 ggtcaggcag gcgagcagcg ccacgcagcc gagcgccagc gcgtagagct gggtccagaa
     2581 ctcacgcccg gcatgtccgc tcccgtcggg caggcgcacg taggtcgtgc tgtagatcat
     2641 cgccgcgaac gcgcggccgt cagccgtcgg ccctcagccg tcagcggacg ccgcaaccat
     2701 ccggcggtga tggggtcttc cgcctgacca gcctccgcta aag
//
LOCUS       DASRIQ010000173        12052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2687950, whole genome shotgun sequence.
ACCESSION   DASRIQ010000173 DASRIQ010000000
VERSION     DASRIQ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687950"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VFD06_04230"
     CDS             complement(<1..1369)
                     /locus_tag="VFD06_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02778.1"
                     /translation="MPRNDHARFQAQLGRLLESIDDPVKIYRTALAQAGRFLDATGGA
                     VILRNPVSDTLKTYGWGADRGAWDEKTVRAFIALERPEIPPRALLAPIVVGGRAIGVL
                     AIARKEGSFAEGAGRFLVEVGRKVAHEITLREERRLNRVVDQIKDRTVRDLRPKDLFY
                     QVLHGLRSLTRYDHSSALLIAEDEGARLVLHAEQIAWEKQKSRRIGRKVHVPDPVRGL
                     LRRPGVVRVVTFDPPRSRREGGAGGAVEPGGGGAGGAGAASGDAATLGRLLRWGGRGK
                     EPAEGTLLLAPLHHDNELLGLLKIAALRPGAFGDPEAEAVGRVLRHVASVIYLSRRAL
                     SMEERMIESEKKHAMADLARAISHDVKNAIGAILPLAQQAREELQAGAAKPEVLIQDL
                     AQIEQSAQVCQRIFDGMLHLARSGVRPQEKAPLARVLQGPLGILATRIRRAGIALEVR
                     IPDDLP"
     gene            complement(1407..1625)
                     /locus_tag="VFD06_04235"
     CDS             complement(1407..1625)
                     /locus_tag="VFD06_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1584..1586),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HZN02779.1"
                     /translation="MANKVVRIFGKDTUPYTTSAREAFADKQYDVKYLDVLSDKALLD
                     EMLGLTKGGRRVPVIVEEGKVTIGYGGS"
     gene            complement(1653..1725)
                     /locus_tag="VFD06_04240"
     tRNA            complement(1653..1725)
                     /locus_tag="VFD06_04240"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1690..1692),aa:Lys,seq:ctt)
     gene            complement(1742..1817)
                     /locus_tag="VFD06_04245"
     tRNA            complement(1742..1817)
                     /locus_tag="VFD06_04245"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1782..1784),aa:His,seq:gtg)
     gene            complement(1855..1930)
                     /locus_tag="VFD06_04250"
     tRNA            complement(1855..1930)
                     /locus_tag="VFD06_04250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1895..1897),aa:Arg,seq:tct)
     gene            complement(2014..2090)
                     /locus_tag="VFD06_04255"
     tRNA            complement(2014..2090)
                     /locus_tag="VFD06_04255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2054..2056),aa:Arg,seq:ccg)
     gene            complement(2157..2230)
                     /locus_tag="VFD06_04260"
     tRNA            complement(2157..2230)
                     /locus_tag="VFD06_04260"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2194..2196),aa:Pro,seq:tgg)
     gene            2406..3275
                     /locus_tag="VFD06_04265"
     CDS             2406..3275
                     /locus_tag="VFD06_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008221735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN02780.1"
                     /translation="MIREKERTAFSGYAALAILLVVLAAAVYGFVQGIIDQNKGMAIA
                     SIVTFVVSVFMMAGLFVVDPNEGRVLQLFGSYVGTVKQPGLRFANPFYSKRRLSLRVR
                     NFESAHLKVNDIEGNPIELAAVVVWRVVDTAEACFEVDSYENYVHVQSEAALRNLATS
                     YAYDAHDEKQMSLRGHTAEIAEHLKTEIQARLNRAGVEVLEARISHLAYAPEIASAML
                     QRQQAGAIIAARQRIVDGAVGMVEMALARLEQNKVVTLDGERRAAMVSNLLVVLCGER
                     SAQPVLNTGTLYQ"
     gene            3279..3476
                     /locus_tag="VFD06_04270"
     CDS             3279..3476
                     /locus_tag="VFD06_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02781.1"
                     /translation="MSERRPFLLRVDPEVLAALERWARDDLRSLNGQIEFLLRRLLRD
                     AGRLKDGEGAPAPPPREPRRR"
     gene            3558..4640
                     /gene="ychF"
                     /locus_tag="VFD06_04275"
     CDS             3558..4640
                     /gene="ychF"
                     /locus_tag="VFD06_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZN02782.1"
                     /translation="MKFGLLGFPKVGKTSLFNILTGARVSVDKYASRSQSNVGVAKVV
                     DPRVARLGEIFKPKKLTYAQFDFIDLQGVQKGEGQQSLSLGEMRNVDAIAHVVRAFQD
                     PEIAHAEGTVDPSRDIATMEMELILADLEVASRRKERLALNLRKAKNKEDELELPIVE
                     RCLAALEKETPIRSLDLKAEDLKRIRGFAFLTAKPLLILVNVDEAAASRVDRVADEWG
                     LKDWTGHPRTAVAGLSAKVEEEIARLDPEDARAFLADLGLAEPAKDRVIHAAFGLLGL
                     IQFLTAGEDECRGWSIASGTRAPQAAGTIHSDIERGFIRAEVVAFDDLVGAGSLAAAR
                     EKALLRSEGKDYVVRDGDVINFRFNV"
     gene            complement(4630..6084)
                     /gene="selA"
                     /locus_tag="VFD06_04280"
     CDS             complement(4630..6084)
                     /gene="selA"
                     /locus_tag="VFD06_04280"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZN02783.1"
                     /translation="MGSPSTLRRKGSRPPSRAGALRTLPSVHEVLERPEVRALCERHG
                     RPVVVALLRERLAALRQGTGRRAAGVADLASAVDALPAWLEAEAIGRTRSSLVPVINA
                     TGVVIHTNLGRAPLPEAALRRMAEVGGSYSTLEYDLREGGRGSRGVHLERLFRFLFPE
                     AATLVVNNNAAGVLLALNSLAEGREVIVSRGELVEIGGSFRIPEILAKSGAVLREVGT
                     TNKTRLADYEKAIGPRTALLLKVHPSNYRIVGFTQSVSLADLARLGRRRRVPILMDQG
                     SGNLHDLRRHGVRGEPTVGEALADGADLVCASADKLLGGPQAGLLLGRPALVERLRKN
                     PLARALRVDKTIVAALEAVLLAHVRGTPETLPAFRMIALAPGDLERRARSFVERLERL
                     AGSGVRARVRPGASVTGGGSGPGEGLPSALVAIRADGTSARVLEERLRRGERPVIGRI
                     EAGELLLDLRTVRDEEEPALEAAVVAAAAADLRR"
     gene            6202..6855
                     /locus_tag="VFD06_04285"
     CDS             6202..6855
                     /locus_tag="VFD06_04285"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HZN02784.1"
                     /translation="MALEEELKALDFEIRRLKVQYDLYFQGAVPRPPTDQHGSLSRAM
                     RKLQGVELRNLSERFMYNNIVNKFNTFQELWNKMMRMKEEGARVHPLAARAAKRAAGA
                     ANGGSAGPPTPARAPAAPGPTPKAAPAASPSGSFRIPSKGQDEASVRRLYETFVAARR
                     DAGDARPLAFETFSREISRQTAAMQSKGLCEAVEFKIYSRDNKVTLKVKAVAPKEGS"
     gene            6885..7550
                     /locus_tag="VFD06_04290"
     CDS             6885..7550
                     /locus_tag="VFD06_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02785.1"
                     /translation="MRFAAAAALLAAWSVVSAQEPQAPPPAPPSGAPAVAPEETAPEP
                     APAPATPKPPARKPDNAVPLDIDVLSEKLARYPDNPYLLNELGNLLLRQGRRLDAEAR
                     YLRAVKLDKDFAAGWNNLGVVRFSLGKKSDAKLAYRKAVAIQPNYALAWYNLGVCLDS
                     LNYYTQAIKAYQRAFVLDPGLLDVRQNPQVASNNRIPAVMAQTYIDRGGTVVFPVDSS
                     YPE"
     gene            complement(7570..9672)
                     /gene="rhaD"
                     /locus_tag="VFD06_04295"
     CDS             complement(7570..9672)
                     /gene="rhaD"
                     /locus_tag="VFD06_04295"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF006189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HZN02786.1"
                     /translation="MQSLWRDEEARGLSGLDLLVYRSRLLGRDPALVLWGGGNTSLKH
                     QEHDFRGRPVAVLRVKGSGADLRTIGPGDFPGLRLDDLLLLQERASMDDDAMVAWLMQ
                     CLMEPQARRPSIETLLHAFVPHPHVDHTHADAILALADQRDARRHVEAAFGEQAVFIP
                     YRRPGFLLSKEVGEAVRGAPRAHAAVLERHGLITWGSGAKESYETTLSVLTRAEEYAG
                     DRARGRVVLGPLTQPPLEPQARRRLAARLLPRLRGILSRVPVPGEAEGPGSRVVLRWD
                     DSDDLLDFVGREAAPALALAGPATPDHLMYTRVRPAIVALHDAPPAGEEDTDRLAAAC
                     EREVAKFADWYRLFVRKHGGAAAAADPRPRVLLVPGLGAIVAGRDARHARIVADVLRH
                     GTQVMMAAAGLGEYRSLTLQEAHDIEFWPLELYRMSLAPKEAELSRRVALVTGAARGI
                     GRAAALRLAEAGCHVAVTDRDAEGADAVAEEIRARHGTDRALGVRLDVTDEASVAAGF
                     EAAALAWGGLDIVVSSAGIAHVSPVERLERSDWDRVMLVNATGHFLVSREAVRILKAQ
                     GTGGSIVVIGSKNVLAPGKDFGAYSASKAAQVQLARVLAIEAGSDRIRVNLVHPDAVF
                     QDSGLWSDEVRQERARAHGIAPEGLEEFYRKRNLLGEKVTAEDVAEAVLWLASDRSLK
                     TTGCILTVDGGLREAFPR"
     gene            complement(9679..9897)
                     /locus_tag="VFD06_04300"
     CDS             complement(9679..9897)
                     /locus_tag="VFD06_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZN02787.1"
                     /translation="MRHNGRVKWFNASRGYGCIALDGGQEVTVNSAAILREGVRTLEE
                     GQAVECDVAQGPQGPHAERVVPIGEIPE"
     gene            10045..10875
                     /locus_tag="VFD06_04305"
     CDS             10045..10875
                     /locus_tag="VFD06_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02788.1"
                     /translation="MNRLRIFVLVAAIVSLSFAPAMAWTDETRLRMLADALKVAPPGL
                     AAILKKHRKDLERGMTVPSKHEEEEVHFLRAEGGGLAADAVILKTKQAKDLLSKKGTL
                     DRFAFEAGTLAHLVSDVSFPLNASDADPREPLIREGYRTLIDRWLDKIPFVLDQAPSP
                     LAKSGDLRTYLQESARRAARSYARISPAFKDDGTPVTPQAVDERSVPFGVSALAYSQA
                     VNDVARVWTLLWREAGGDMTGAGPLVATTPPVYGPVQPPVPTAAKPPAPAKPPDAGKP
                     "
     gene            10872..>12052
                     /gene="purH"
                     /locus_tag="VFD06_04310"
     CDS             10872..>12052
                     /gene="purH"
                     /locus_tag="VFD06_04310"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZN02789.1"
                     /translation="MSPLPPRALLSVYDKTGIADLARRLAGLGFQILSTGGTAETLRR
                     EGIEVTDVSRHTGQPEILGGRVKTLHPRIHGGLLGDPSEPAHRADMERSGIEPIGLAV
                     VNLYPFRSVAAKENAPLADLVEMIDIGGPAMIRSAAKNHAAVGVVVDPADYESVAEEI
                     TSTGGLSEATRRRLAAKAFAHTAAYDADIRDTLHRRFAALDSAPDSGDAAQAGFPDTL
                     RLEFTRRQSLRYGENPHQRGALYVAPGARGGTVARARQIQGKELSYNNLLDLDAAWRL
                     VREFSAPAAVIVKHNNPCGAACAPDLAAAFDAARRTDPVSAFGGILAFNRELDAEAAA
                     AATGLFLECVIAPSVAAAARPILAAKTGLRVLEAGLPAASPDGALDLRSISGGLLAQD
                     G"
BASE COUNT         1813 a         4427 c         4136 g         1676 t
ORIGIN      
        1 ccgggaggtc gtccgggatc cggacctcga gcgcgatgcc ggcgcgccgg atgcgtgtcg
       61 cgaggatgcc gagcgggccc tggagaacgc gggccagggg cgccttttcc tgggggcgca
      121 cgccggagcg cgccagatgc agcatgccgt cgaagatcct ctggcagacc tgggcggact
      181 gctcgatctg cgccaggtcc tggatcagca cctccggctt ggcggccccg gcctgcagct
      241 cctcgcgcgc ctgctgcgcc aggggcagga tggcgccgat ggcgttcttg acgtcgtgcg
      301 agatggcgcg cgcgagatcc gccatcgcgt gcttcttctc gctctcgatc atccgctcct
      361 ccatcgagag cgcgcggcgc gaaaggtaga tcaccgaggc gacgtgccgg aggacgcggc
      421 cgaccgcctc cgcctcggga tcgccgaaag cgccgggacg aagcgccgcg atcttgagaa
      481 gccccagcag ctcgttgtcg tggtgcagcg gcgcgaggag cagcgttccc tccgccggct
      541 ccttgccgcg cccgccccag cgcaggagac gcccgagcgt cgccgcgtca ccgctggcgg
      601 ctcccgcgcc gcccgcgccg ccgccacccg gctcgaccgc cccgccggcg ccgccctcgc
      661 gccgcgatcg cggcggatcg aacgtgacga cccgcacgac gcccggccgg cgcagaagcc
      721 cgcgcaccgg atccggcacg tggaccttgc ggccgatccg ccggctcttc tgcttctccc
      781 acgcgatctg ctcggcatgc aggacgagcc gcgcgccttc gtcttcggcg atcaggagcg
      841 ccgaggagtg gtcgtagcgc gtcagcgatc ggagaccgtg cagcacctgg taaaagaggt
      901 ccttcggacg cagatcgcgc accgtacggt ccttgatctg atcgacgacg cggttgaggc
      961 ggcgctcctc gcgcaacgtg atctcgtgcg cgaccttgcg cccgacctcc accaggaacc
     1021 gcccggcgcc ctcggcgaac gacccctcct tccgggcgat cgccagaacg ccgatggcgc
     1081 gcccgccgac cacgatcggc gccagaagcg cccggggcgg gatctccggc cgctcgagcg
     1141 cgatgaaggc gcgcacggtc ttctcgtccc aggcgccccg gtcggcgccc cagccatagg
     1201 tcttgagggt gtcggaaacc ggattgcgga ggatcaccgc gccgccggtc gcgtcgagga
     1261 agcgaccggc ctgcgccagc gcggtgcggt agatcttgac gggatcgtcg atcgactcga
     1321 gcagacgccc gagctgggcc tggaagcgcg cgtgatcgtt cctgggcatc aatgactccg
     1381 gccgccggcc ggagagctca gacgcctcag gagccgccgt agccgatggt gaccttgccc
     1441 tcttcgacga tgaccggcac ccgccggccg cccttggtca ggccgagcat ttcgtcgagg
     1501 agcgccttgt cggagagcac atccagatac ttcacgtcgt actgcttgtc ggcgaaggcc
     1561 tcacgggccg acgtcgtgta gggtcaggtg tccttgccga agatccggac gactttgttg
     1621 gccatcgcga cacctcaaaa gaagaagatc tgtgagccgt ggaggactcg aacctccgac
     1681 ccgctgatta agagtcagct gctctaccga ctgagctaac ggcccaattc ccgtggaaat
     1741 ctggggtgag tgacggggtt cgaacccgcg acaaccggag ccacagtccg gggctctacc
     1801 agctgagcta cactcaccat catttcaacg ccgactgccg cacgaccgcc aggatggtgc
     1861 gcctgaaggg attcgaaccc ttgacccacg gcttagaagg ccgttgctct gtcccctgag
     1921 ctacaggcgc gcagtcggaa cttcgtttcc acaacggccc gacgccacga tccggacctt
     1981 ttggggagcg gcatgttaac acgacgtccg ggctggcgcg cctgagagga ttcgaacctc
     2041 cgacccccag ctccggaggc tggtgctctg atccgctgag ctacaggcgc gcacccgagg
     2101 cctgacgaca gggagaccgg accggaaccg gccgtcgtcg ccgcgcggat ccggggtcgg
     2161 ggcgagtgga ttcgaaccac cgaccctctg ctcccaaagc agatgcgcta ccaggctgcg
     2221 ctacgccccg gagtacccga actgccgccg acgaggcggg attctatcag agaccgtgca
     2281 ccaaacctcg cggctgggtg cctgggtttt ccgcttgaca cttgacaatg gtgattgcat
     2341 gttgatatca cttcgcaatc acaggacagc ttgaggccgc gggagcggcg gagggagaac
     2401 gggacatgat tcgggaaaag gaacggacgg cgttctctgg ctatgcggcc ctggcgatcc
     2461 tgctggtggt gctggccgcg gcggtctacg ggttcgtcca gggaatcatc gaccagaaca
     2521 aggggatggc gatcgcgtcg atcgtgacgt tcgtcgtctc cgtcttcatg atggccgggc
     2581 tgttcgtggt cgatccgaac gaggggcggg tcctgcagct cttcgggtcc tacgtcggga
     2641 cggtcaagca gccggggctg cggttcgcca acccgttcta cagcaagcgg cgcctgtcgc
     2701 tgcgggtgcg caacttcgag tcggcccacc tgaaagtcaa cgacatcgag gggaacccga
     2761 tcgagctggc cgcggtcgtc gtctggaggg tggtggacac cgccgaggcc tgcttcgagg
     2821 tggacagcta cgagaactac gtccacgtgc agagcgaggc ggcgctccgg aacctcgcga
     2881 cctcctacgc ctacgacgcc cacgacgaga agcagatgtc gctgcgcggc cacacggcgg
     2941 agatcgccga gcacctcaag acggagatcc aggcgcggct gaaccgcgca ggggtcgaag
     3001 tcctggaggc gcgcatcagc cacctggcgt acgcgccaga gatcgcgtcc gccatgctgc
     3061 agcgccagca ggcgggggcg atcatcgccg cgcggcagcg gatcgtcgac ggcgccgtcg
     3121 gcatggtgga gatggcgctc gcccggctgg agcagaacaa ggtcgtcacc ctcgacggcg
     3181 agcggcgcgc cgccatggtc agcaacctcc tcgtcgtcct gtgcggcgag cggtcggcgc
     3241 agccggtcct gaacaccgga acgctctatc aatagccggt gagcgaacgg cgcccgttcc
     3301 tgctgcgcgt cgatccggag gtcctcgccg cgctcgaacg ctgggcccgg gacgatctcc
     3361 gcagcctcaa cggccagatc gagttcctgc tgcggcgcct gctgcgcgac gccggccggc
     3421 tcaaggacgg cgagggggcc cccgcccccc cgccgcgcga gccgcgccgg cgctgacgca
     3481 cgcgaaatcc gggctagaat ccgcccttcg cgccggcggc gcgccccgcg cccgccggcc
     3541 ctccgaggga cacgacgatg aagttcggac tgctgggctt ccccaaggtg gggaagacca
     3601 gcctcttcaa catcctgacc ggcgcgcgcg tcagcgtcga caagtacgcc tcgcggtcgc
     3661 agtcgaacgt cggcgtcgcc aaggtcgtcg atccccgcgt cgcccggctc ggggagatct
     3721 tcaagccgaa gaagctcacc tacgcgcagt tcgacttcat cgacctgcag ggggtgcaga
     3781 agggggaagg gcagcagtcg ctgtccctcg gcgagatgcg caacgtggac gccatcgccc
     3841 acgtcgtccg cgccttccag gacccggaaa tcgcccacgc cgagggcacc gtcgacccgt
     3901 cgcgcgacat cgcgacgatg gagatggaac tcatcctcgc cgatctcgag gtggcgtcgc
     3961 gccgcaagga gcgcctggcg ctcaacctcc ggaaagcgaa gaacaaggag gacgagctcg
     4021 agctgccgat cgtcgagcgc tgcctcgcgg cgctcgagaa ggagacgccg atccgctcgc
     4081 tcgatctcaa ggcggaggac ctcaagcgga tccgcggctt cgctttcctg accgccaagc
     4141 cgctcctcat cctggtcaac gtggacgagg cggcggcctc gcgcgtcgac cgggtcgcgg
     4201 acgaatgggg attgaaggac tggaccggcc acccacggac ggcggtggcc ggcctgtccg
     4261 ccaaggtcga agaggagatc gcccggctcg accccgagga tgcgcgcgcc ttcctcgccg
     4321 atctcggcct cgccgagccg gccaaggacc gcgtcatcca cgccgccttc ggcctcctcg
     4381 ggctgatcca gttcctgacg gccggggagg acgaatgccg cggctggtcg atcgcgtccg
     4441 gcacgcgcgc gccgcaggcg gccgggacga tccacagcga catcgagcgg ggcttcatcc
     4501 gggcggaagt cgtcgccttc gacgacctcg tcggggcggg gagcctcgcg gcggcgcgcg
     4561 agaaagccct cttgaggtcc gaagggaagg actacgtcgt gcgggacggc gacgtcatca
     4621 acttccgctt caacgtctga gatccgccgc cgccgcggcg acgaccgccg cctcgagcgc
     4681 cggctcctcc tcgtcgcgga ccgtgcgcag atccaggagc agctcccccg cctcgatccg
     4741 cccgatcacc ggccgctcgc ccctccgcag ccgctcctcg aggacgcgcg cgctcgtccc
     4801 gtccgcccgg atcgccacga gcgccgaagg gagtccctcc cccgggcccg atcccccgcc
     4861 ggtcaccgac gcccccggcc gcacgcgcgc ccgcactccc gacccggcga ggcgctcgag
     4921 acgctcgacg aaggaccgcg cccgccgctc cagatcgccc ggagcgaggg cgatcatccg
     4981 gaacgccggc agagtctccg gcgtcccgcg cacgtgggcg aggaggaccg cttcgagcgc
     5041 ggcgacgatc gtcttgtcca cccgcagcgc gcgcgcgagc gggttcttgc gcagccgctc
     5101 cacgagcgcc ggccgcccga ggaggagacc ggcctgcggc ccgccgagga gcttgtcggc
     5161 gctggcgcac acgagatccg cgccgtctgc gagcgcctcc cccaccgtcg gctccccccg
     5221 cacgccgtgc cgtcgcaggt cgtgcaggtt gccgctcccc tgatccatga gaatcggcac
     5281 gcggcgccgc cgcccgagcc gcgcgagatc ggcgagggac accgactgag tgaatccgac
     5341 gatccggtag ttgctcgggt gcaccttgag gaggagggcg gtgcgggggc cgatcgcctt
     5401 ctcgtagtcg gcgagacggg tcttgttcgt cgttcccact tcgcgcagga cggcgccgct
     5461 cttcgcgagg atctccggga tccggaagga gccgccgatc tccaccagct ccccgcgcga
     5521 gacgatcacc tcgcgcccct ccgccaggct gttgagcgcg aggagcacgc cggccgcgtt
     5581 gttgttcacc acgagcgtcg cggcctccgg gaacaggaag cggaacagcc gctcgaggtg
     5641 cacgccgcgc gagccgcgcc ccccctcgcg gagatcgtat tccagggtcg agtaggagcc
     5701 tccgacctcc gccatgcggc gcagcgccgc ctccgggagc ggcgcccggc cgaggttggt
     5761 gtggatgacg acgccggtcg cgttgatcac cggcacgagg ctcgagcggg tgcgcccgat
     5821 cgcttcggcc tccagccagg ccgggagcgc gtcgaccgcc gacgcgagat cggccactcc
     5881 cgctgcccgg cgcccggtcc cctggcggag cgcggcgagc cgctcccgca ggagcgccac
     5941 cacgaccggc cggccgtgcc gctcgcacag ggcgcgcacc tccgggcgct ccagcacctc
     6001 gtgaaccgaa ggcagcgtgc gcaaagcccc ggcccgggag ggggggcgcg atccctttct
     6061 tcgcagcgtg gagggggatc ccatgcgggg tggcatcaca tcacagtcat cgggaggtgt
     6121 caaaagcccg acgttttgaa acgtcgggac gcgcaaccta tattcggcgc gcggctcggc
     6181 cgcgacgagg acgtctgaca catggcgttg gaagaggaac tgaaggcgct cgacttcgag
     6241 atccgccgcc tcaaggtcca gtacgacctt tacttccagg gcgcggtccc gcgtcccccg
     6301 accgaccagc acggctccct gtcccgcgcg atgcgcaaac tccagggagt ggagctgcgc
     6361 aacctgtccg agcgcttcat gtacaacaac atcgtcaaca agttcaacac gttccaggaa
     6421 ctctggaaca agatgatgcg gatgaaggag gagggggcgc gcgtgcaccc gctcgcggcc
     6481 cgcgccgcga agcgcgccgc cggcgccgca aacggcggca gcgccggccc ccccaccccc
     6541 gcacgcgcac cggccgcccc cggcccgacc cccaaggcgg ctccggccgc ctccccctcc
     6601 ggctccttcc ggatcccctc gaagggccag gacgaggcct ccgtgcggcg gctgtacgaa
     6661 acgttcgtgg cggcgcgccg cgacgcgggg gacgcccgtc cgctggcgtt cgagaccttc
     6721 tcgcgcgaga tctccaggca gacggccgcc atgcagtcga agggcctgtg tgaggcggtg
     6781 gagttcaaga tatactcgcg cgacaacaag gtgactctca aggtgaaagc cgtggccccg
     6841 aaggagggtt cgtgaacgaa ggatggagga acggagcgcg gggcatgcgg ttcgccgctg
     6901 cggcggcgct tctcgccgcg tggtccgtgg tgtccgccca ggagccgcag gctcctccgc
     6961 ccgcgccccc ctccggcgct cccgctgtcg cgcccgaaga aaccgcgccg gagccggccc
     7021 cggctcccgc gaccccgaag ccgccggcgc gcaagccgga caatgccgtc ccgctcgaca
     7081 tcgacgtgct gtcggagaag ctcgcgcgct atcccgacaa cccctacctg ctcaacgagc
     7141 tggggaatct gctgctgcgc caggggcggc ggctcgatgc ggaggcccgt taccttcgcg
     7201 ccgtcaagct cgacaaggac ttcgccgccg gctggaacaa cctcggcgtc gtgcggttct
     7261 ccctggggaa gaagagcgac gcgaagctcg cataccgcaa ggcggtggcg atccagccga
     7321 actacgccct cgcctggtac aacctcggag tctgcctcga ctcgctgaac tactacacgc
     7381 aggcgatcaa agcgtatcag cgggcgttcg tgctcgatcc ggggctcctc gacgtccgcc
     7441 agaatcccca ggtggcgagc aacaaccgga tcccggcggt gatggcgcag acctacatcg
     7501 accgcggcgg gacggtggtc ttcccggtcg actcctccta cccggagtag ccggcgtccg
     7561 tcgcgcgcgt cagcgcggaa acgcctcccg cagtcccccg tccacggtca ggatgcatcc
     7621 ggtcgtcttg agcgaccgat ccgaggcgag ccacaagacc gcctcggcga cgtcctcggc
     7681 ggtgaccttc tccccgagca ggttgcgttt ccggtagaac tcctcgagcc cttcgggagc
     7741 gatcccgtgg gcgcgcgccc gctcctggcg cacctcgtcc gaccagagcc ccgaatcctg
     7801 gaacacggcg tccgggtgga cgaggttgac ccggatgcga tccgatccgg cctcgatcgc
     7861 caggacgcgc gccaattgca cctgcgccgc cttcgaggcg ctgtacgctc cgaagtcctt
     7921 ccccggggcg aggacgttct tcgagccgat cacgacgatg ctcccgccgg tcccctgcgc
     7981 cttgaggatg cgcaccgcct cgcgcgagac gaggaagtgc ccggtcgcgt tcaccagcat
     8041 caccctgtcc cagtcgcttc gctccaggcg ctccaccggg ctgacgtggg cgatgccggc
     8101 gctgctgacg acgatgtcga gcccgcccca ggcgagcgcc gccgcctcga acccggcggc
     8161 gaccgacgcc tcgtcggtca cgtcgagccg cacgccgagg gcgcggtccg tcccgtgccg
     8221 agcgcggatc tcctccgcca ccgcgtccgc cccctcggcg tcccgatcgg tcacggccac
     8281 gtggcagccg gcctcggcga gcctcagcgc cgccgctcgt ccgatgccgc gcgccgcacc
     8341 cgtgaccagt gcgacgcggc gcgaaagctc cgcctccttc ggcgcgagac tcatgcggta
     8401 cagctcgagc ggccagaact cgatgtcgtg cgcctcctgg agtgtcagcg agcgatactc
     8461 gccgagtccc gccgcggcca tcatcacctg cgtcccgtgc ctgaggacgt ccgcgacgat
     8521 gcgtgcgtgc cgcgcgtccc ttccggcgac gatcgccccg agccccggga cgagcagcac
     8581 gcgcggccgc ggatcggccg cggcggcggc gccgccgtgc ttgcggacga agagccggta
     8641 ccagtcggcg aacttggcga cctcacgctc gcaggccgcg gcgagacggt ccgtgtcctc
     8701 ctcccccgcc ggcggcgcat cgtgcagcgc cacgatcgcc ggccggaccc gcgtgtacat
     8761 caggtgatcc ggagtcgccg gccccgcgag cgccagcgcc ggcgccgcct cacggccgac
     8821 gaaatcgagc aggtcgtcgg agtcgtccca gcgcaggacg acgcgggagc ccggcccctc
     8881 cgcctccccg gggactggga cgcgcgacag gatgccccgg agccgcggca ggaggcgcgc
     8941 ggcgagccgc cggcgcgcct gcggctcgag cggcggttgc gtcagcggcc ccagcacgac
     9001 gcgcccgcgg gcccgatcgc ccgcgtactc ctccgcgcgc gtcaggacgg agagggtcgt
     9061 ctcgtacgac tctttcgcgc cggatcccca cgtgatgagc ccgtggcgct cgagcaccgc
     9121 cgcgtgggcg cgcggcgctc cgcgcaccgc ctcccccacc tccttcgaca acaggaagcc
     9181 ggggcgccgg taggggatga agaccgcctg ctcgccgaac gccgcctcga cgtggcgccg
     9241 ggcgtcccgc tggtcggcga gggcgaggat ggcgtcggcg tgcgtgtgat cgacgtgcgg
     9301 atgcggcacg aaggcgtgca gcagcgtctc gatcgacggc cggcgcgcct gcggctccat
     9361 gaggcactgc atgagccacg ccaccatcgc atcgtcgtcc atggaggcgc gttcctggag
     9421 gaggagcaga tcgtcgagcc gcagtccggg gaaatcgccg gggccgatcg tcctgaggtc
     9481 ggcgccgctc cccttgaccc ggagcaccgc gaccgggcgg ccgcggaagt catgctcctg
     9541 gtgcttgagc gaggtgttgc cgccgcccca caggacgagc gccgggtcgc ggccgaggag
     9601 ccgcgatcgg tacaccagga gatcgaggcc ggagaggccg cgggcctcct cgtcgcgcca
     9661 cagactctgc atgccgtgct actcggggat ctccccgatc gggacgaccc gctcggcgtg
     9721 cggcccctgc gggccctggg cgacgtcgca ctcgaccgcc tgcccttcct cgagtgtgcg
     9781 cactccctcg cgcaggatcg cggcgctgtt caccgtcacc tcttggccgc cgtcgagggc
     9841 gatgcacccg tagccgcggc tggcgttgaa ccacttcacg cgcccgttgt gtctcatcgg
     9901 tgcggtatcc tgcacggccc tcttgcggtt gtcaacgcgg aggacctcct ggtcgccagg
     9961 gtccgggagg ccgggggcag gcccgcgtcc ccgttccctc tgaggcgtcg actgcgactc
    10021 gttcgccgca tgtaaaatgc ctccgtgaat cgcttgcgca ttttcgttct cgtcgccgcg
    10081 atcgtgtcgc tgtcgttcgc gccggcgatg gcctggacgg atgagacgcg cctgcgcatg
    10141 ctggccgacg cgctgaaggt ggcgccgccg ggcctcgcgg cgatcctcaa gaagcaccgc
    10201 aaggacctgg agcgcggcat gacggtcccc tcgaagcacg aggaagagga agtgcacttc
    10261 ctgcgcgccg aggggggcgg cctggcggcc gacgccgtga tcctcaagac caagcaggcg
    10321 aaggacctgc tctcgaagaa gggaacgctc gaccgcttcg cgttcgaggc ggggacgctc
    10381 gcccatctgg tgtccgacgt ctcgttcccg ctcaacgcct ccgatgccga tccgcgcgag
    10441 ccgctcatcc gcgaggggta ccggaccctg atcgatcgct ggctcgacaa gatcccgttc
    10501 gtcctcgacc aggcgccgtc accgctcgcc aagagcggcg acctgcgcac gtacctccag
    10561 gagagcgcgc ggcgcgccgc ccgctcgtac gcgcgcatct cccccgcctt caaggacgac
    10621 ggcacgccgg tgacgccgca ggcggtggac gagcggtcgg taccgttcgg cgtctccgcc
    10681 ctcgcctact cgcaggcggt gaacgacgtg gcgcgggtgt ggacgctgct gtggcgggag
    10741 gcgggtgggg acatgaccgg agcggggccg ctggtcgcca cgacaccgcc ggtctacggc
    10801 cccgtccagc cgcctgtccc caccgcggcg aagcctcccg ccccggcgaa gcctcccgac
    10861 gcggggaagc cgtgagcccg ctgccgcccc gcgccctgct gagcgtgtac gacaagaccg
    10921 ggatcgccga tctcgcgcgc cggctcgccg gcctcggctt ccagatcctc tccaccggcg
    10981 ggacggccga gaccctgcgg cgcgagggga tcgaggtcac cgacgtctcg cggcacaccg
    11041 gccaaccgga gatcctcggc ggccgcgtca agacgctgca cccgcgcatc cacggcggcc
    11101 tcctcggcga tccgtcggag cctgcgcacc gcgccgacat ggagcgctcc ggcatcgagc
    11161 cgatcggcct ggcggtcgtc aacctgtacc ccttccgatc ggtggccgcg aaggagaacg
    11221 cgccgctcgc cgatctcgtc gagatgatcg acatcggcgg gccggcgatg atccgctcgg
    11281 ccgccaagaa ccacgcggcg gtcggggtgg tcgtcgaccc agccgactac gaatccgttg
    11341 cggaggagat cacctccacc ggcgggcttt ccgaggccac ccggcgccgg ctggcggcga
    11401 aggcgttcgc ccacaccgcc gcctacgacg ccgatatccg ggacacgctg caccggaggt
    11461 tcgcggcgct cgactccgca ccggactccg gagacgcggc ccaggcgggc ttcccggaca
    11521 cgctgcgcct cgagttcacg cggcgacagt cgctccgcta cggggagaac ccgcaccagc
    11581 ggggcgccct ttacgtcgct cccggcgcgc gcggagggac ggtggcgcgg gcgcggcaga
    11641 tccaggggaa ggagctgtcc tacaacaacc tgctggacct ggacgccgcc tggcggctgg
    11701 tgcgcgagtt ctccgccccc gcggcggtca tcgtgaagca caacaatccg tgcggcgccg
    11761 cctgcgctcc ggacctcgcg gcggcgttcg atgcggcgcg ccgcaccgat ccggtctccg
    11821 cgttcggcgg catcctcgcc ttcaaccggg agctggatgc cgaggcggcg gccgcggcga
    11881 ccggcctctt cctcgagtgc gtgatcgccc catcggtggc ggccgccgcc cgcccgatcc
    11941 tcgcggcgaa gacggggttg cgggtgctcg aggcgggcct ccccgcggcg tccccggacg
    12001 gcgcgctcga cctccgcagc atctcggggg gcttgctcgc gcaggacggc ga
//
LOCUS       DASRIQ010000174         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1880198, whole genome shotgun sequence.
ACCESSION   DASRIQ010000174 DASRIQ010000000
VERSION     DASRIQ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880198"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFD06_04315"
     CDS             complement(<1..385)
                     /locus_tag="VFD06_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02790.1"
                     /translation="MPRRTPLFARLASVLARGAAGLVLAGLVGAPGTAAAATPGEATN
                     LMVTGYDPVTGDIAISWTNGCGAADHHIEYGPLGSISTYGYSGQVCGLGTGGSYVSFD
                     PGPDSYFFFIVADDGASVEGSYGTSW"
     gene            complement(518..1654)
                     /locus_tag="VFD06_04320"
     CDS             complement(518..1654)
                     /locus_tag="VFD06_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02791.1"
                     /translation="MRATVLNDAALAKHAGRFAWLSINTEQSRNAEFVEHLAVGAYPT
                     FLVLDGATGNVALRWAGSLTAPEFERLLDDGERAVAASGGGAPADAALARADRLGAAG
                     KTAEAAAVYTEALRLAPAEWGGRGRALASLLAALQKSGDLEQCAALARRETPPLERGP
                     AFAGASAAGLGCAVDAPEGAAWRVAAIQELEALVVESLALDNVLADDRSSAYGMLVDL
                     HDARGDKAGAKEMAGRWLTFLENQAAAAGSVEARAAFDSHRVGAALALGDPARAVPAL
                     LASERDLPDDYNPPARLAILYREQGRFDEALAASRRALDKAYGARKLRIFDTRADIYT
                     KKGDPAAARRTLEEALDYADSLPKSQQPQAFLAQLRKKAAAIGS"
     gene            complement(1670..1894)
                     /locus_tag="VFD06_04325"
     CDS             complement(1670..1894)
                     /locus_tag="VFD06_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02792.1"
                     /translation="MWTTRAPIVGLMLALIAPACAERPSPDAANAAAARTGKAAVAGA
                     LRFIEDDAPTALAEAKRRGVPIFVEAWAPW"
     gene            2092..2739
                     /locus_tag="VFD06_04330"
     CDS             2092..2739
                     /locus_tag="VFD06_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02793.1"
                     /translation="MCRRLRRFILLQALLVATSFPSSAGYRGYVERPWTPTTCQQETH
                     FLTDPCTGNAQWLLTDQYGGIPMDPYLCTYVDVDGPDVGVECTVIQPMSVLAAQPSCP
                     ITFRSLWIDAATPPRLDWYRVPCAGTYDVIRGTLPGPASDGSRINLGPVTCLANDFPQ
                     IDFWYVSGPVDPESPPLGKAFFYLVRAAGLPAGDMTYGHSSDGLEEVPLSGDCSP"
     gene            complement(2750..3004)
                     /locus_tag="VFD06_04335"
     CDS             complement(2750..3004)
                     /locus_tag="VFD06_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02794.1"
                     /translation="MIFGDRNRERERERLIEEAVSAWRPRTPDGRILAHPSWADLPEA
                     DRLRAHDETLRARALEGLIDRQGLSTTVRAVLRRIGARPD"
     gene            complement(3001..3276)
                     /locus_tag="VFD06_04340"
     CDS             complement(3001..3276)
                     /locus_tag="VFD06_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02795.1"
                     /translation="MPAIVETEQDTNHRGCGGRLVSLDGRSLPLRKLPRAPRRAIEMR
                     IAAAGGESDTSLAAHLGMRLNTFLQNVTRARRLLAQCLEKAGIVVPA"
BASE COUNT          479 a         1253 c         1263 g          515 t
ORIGIN      
        1 tccaggaggt tccgtacgaa ccctcgacgc tggcgccgtc gtcggccacg atgaagaaga
       61 agtacgagtc cggccccggg tcgaacgaca cgtacgaccc gccggtcccg aggccgcaca
      121 cctggccgga atagccgtag gtcgagatgc tcccgagggg gccgtactcg atgtggtggt
      181 ccgcggcccc gcagccgttc gtccaggaga tggcgatgtc accggtcacg gggtcgtagc
      241 cggtgaccat gagattcgtc gcctcccccg gcgtcgcggc cgccgcggtg cccggggcgc
      301 cgacgagacc ggcgagcacg agcccggccg cccccctcgc gagaaccgac gcaaggcggg
      361 cgaaaagcgg cgtccgtcgc ggcatgccgg ggattctgcg gcgaaagggg cggccgcgca
      421 agcggatccg acgaccgact tgcgcctcga ggacggatcc ggccgaccct ccccggcgtc
      481 ccgccgtgca tcgaccgctc ttccgcggcc cggcctctca ggagccgatc gcggcggcct
      541 tcttgcgcag ctgggccagg aacgcctgcg gctgctgcga cttgggcagg ctgtcggcgt
      601 agtccagcgc ctcttcgagc gtgcgcctgg ccgcggccgg gtcgcccttc ttcgtgtaga
      661 tgtcggcgcg ggtgtcgaag atgcgcagct tgcgggcgcc gtacgccttg tcgagcgcgc
      721 gccggctggc ggccagggcc tcgtcgaatc ggccctgctc gcggtagagg atcgccagcc
      781 gggccggggg gttgtagtcg tcggggaggt cgcgctcgga ggcgagcagc gccggcacgg
      841 cgcgggccgg atcgccgagc gccagcgcgg ctccgacgcg gtgcgaatcg aaggcggcgc
      901 gcgcctcgac gctcccggcc gcggccgcct ggttctcgag gaaggtcagc cagcggccgg
      961 ccatctcctt ggcaccggcc ttgtcaccgc gcgcatcgtg caggtcgacg agcatcccgt
     1021 acgcgctcga acggtcgtcg gcgagcacgt tgtcgagggc gagactctcg accacgagcg
     1081 cctcgagctc ttgaatcgcc gcgacgcgcc aggctgcgcc ttcgggcgcg tcgacggcgc
     1141 agccgaggcc ggccgcggag gcgccggcga atgccgggcc gcgctcgaga gggggggtct
     1201 cgcgtcgcgc cagcgcggcg cactgctcga ggtcgccgct cttctgcagc gcggcgagga
     1261 gcgacgccag ggcgcgacca cgaccacccc attccgcggg tgccagacgg agcgcctcgg
     1321 tgtagacggc ggccgcctcg gccgtcttcc ccgcggcgcc caggcggtcg gcgcgagcca
     1381 gggcggcatc ggccggtgcg ccgccgccgg acgcggcaac ggcgcgctcg ccgtcgtcga
     1441 gcagccgctc gaactccgga gccgtcagac tcccggccca gcgcaacgcc acgttcccgg
     1501 tcgcgccatc gagcaccagg aatgtggggt aggcgccgac ggccagatgc tcgacgaact
     1561 cggcgttacg cgactgctcg gtgttgatcg agagccaggc gaaccgcccg gcgtgcttcg
     1621 cgagcgcggc gtcgttgagc acggtcgcgc gcacgaagcg gcaggtgtgt caccaggggg
     1681 cccaggcttc gacgaagatc ggcacaccac gccgcttcgc ctcggccagg gccgtgggcg
     1741 cgtcgtcctc gatgaagcgc agcgcgccgg cgacggccgc cttgccggta cgcgccgcgg
     1801 cggcgttcgc ggcgtccggc gacggccgct cggcgcacgc cggcgcgatg agggcgagca
     1861 tcagcccgac gatgggggcc cgcgttgtcc acatgcgtga aggctaccgc gcagggcgag
     1921 cggggtcaac ggaggacggc gggacctcgc gtccggggcg catcctcagg cgtgtatctt
     1981 ccggttgacg gcctccggtt ctcggcgtat aggtcgcttg agtgcgttcg tcgacgctct
     2041 cccggctcgc cgcgccgctc ccgcgtggca cgaacaggaa ggagaggccc catgtgtcgg
     2101 cgcctgcgcc ggttcatcct cctgcaggct ctgctggtcg cgacctcgtt cccgtcgtcg
     2161 gcgggatacc ggggatacgt cgagcggccc tggacgccca cgacctgcca gcaggagacc
     2221 cacttcctga ccgatccctg cactggaaat gctcaatggt tgctcacgga ccagtacggc
     2281 gggatcccta tggatcctta cctgtgcacc tacgtcgacg tggacgggcc cgatgtcggc
     2341 gtcgaatgca ccgtgataca gccgatgtcg gtcctggcgg cgcagccctc ctgtcccatc
     2401 acgttcagga gtctctggat cgacgctgcg acgcccccgc gcctcgactg gtatcgagtc
     2461 ccctgcgccg gcacctacga cgtcattcgc ggcactctcc cgggaccggc ctccgacggc
     2521 tcgcggatca acctgggtcc ggtgacctgc ctggcgaacg acttccccca gatcgacttc
     2581 tggtacgtgt ccggaccggt cgaccccgaa tccccacccc tcggcaaagc ctttttctac
     2641 ctggtgcgcg ccgccggact tcccgctggc gacatgacgt acggtcattc gagcgacggg
     2701 ctcgaggaag tgccgctctc gggcgactgt tccccctgac gcgaacagct tagtccgggc
     2761 gcgcgccgat ccgccgcagc acggcccgca ccgtagtcga cagcccctgg cggtcaatca
     2821 ggccctcgag ggcgcgggcg cggagggtct cgtcgtgcgc ccgcagccgg tccgcctcgg
     2881 ggaggtcggc ccaggagggg tgcgcgagga tgcggccgtc gggcgtgcgc ggccgccacg
     2941 ccgagacggc ctcctcgatg agacgctcgc gctcgcgttc ccggtttcgg tcgccgaaga
     3001 tcatgcgggc accacgatcc ccgccttctc gaggcactgc gccaggagcc gccgggcgcg
     3061 ggtgacgttc tgcaggaatg tgttcagacg catgccgaga tgggccgcga gactcgtgtc
     3121 gctctccccg ccagcggcgg cgatgcgcat ctcgatcgcc cggcgcgggg cgcgcggcag
     3181 cttgcgcaat ggcaggctcc ggccatcgag gctgacgagc ctgccgccgc atccccggtg
     3241 attcgtgtcc tgttcggttt cgacgatcgc gggcatgttc ggccctccca ccctgataac
     3301 gcgccggggg atggtcttgt gacagcgatc ccggactcac tcgaagacgt tccgggagag
     3361 tatctccgcc cagagcgcct gccaggtgtt ccagcgatgc ccgccgggaa cgcgaaggac
     3421 atgtccggcc ggcagcgatt ctgtgaggag gtcgagggaa gggcccatcg gctcgtcccg
     3481 gccgtagccg agatagaggg tcgggtgtcc
//
LOCUS       DASRIQ010000175        18908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2942878, whole genome shotgun sequence.
ACCESSION   DASRIQ010000175 DASRIQ010000000
VERSION     DASRIQ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 18908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18908
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2942878"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFD06_04345"
     CDS             <1..378
                     /locus_tag="VFD06_04345"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HZN02796.1"
                     /translation="RPAAARRGDPGGRGAPAPRGDAMTPGALAGASIVVPAPDVVARD
                     IAGEHLLVPVRSGIAGVDSIYTADEVGSFLYARLDGRRSAAELARLVSEAYVVPEDRA
                     LADVLAFLRELCDAGLARPAETR"
     gene            375..1661
                     /locus_tag="VFD06_04350"
     CDS             375..1661
                     /locus_tag="VFD06_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN02797.1"
                     /translation="MSGGTATFNAFGARVLEACGGERVPLNGSLELTFRCNLRCVHCY
                     IPDFSGRGEMTTSEIVRILDETADAGCLWMLLTGGEVLSRPDFAEIYRHVKRRGMLPT
                     VFTNGTLLQDRHADLFAEYPPFSLEITLYGMSDATYERTTGMPGGFRRVRAAVERLVE
                     RRVRLTLKAVAMAPLVGELPAMRAWADLLGVPFRYDATIHGRLDGSLQPAAARATPAE
                     VVGLDAADAERRVHWQRFYDQFVKNAPKTDRLLSCGAGLQSFHVDPKGWLWSCEALPL
                     DGYDLRHGTFLEGWNGIVGEVRHRRASAANVCAGCDLKSLCDRCPATAILETGSPDGW
                     IPHYCEITHRRAALLESESGDPERAARYLAHAEKVAAGWTPPGVTLPRASRLPQAPSC
                     GTGCGAACGRESSGARPVRSALLSIERPETSREILR"
     gene            1839..2768
                     /locus_tag="VFD06_04355"
     CDS             1839..2768
                     /locus_tag="VFD06_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02798.1"
                     /translation="MAPDAFRPLVLEIAGLGLEVKPAEPGIRLVTDAAHQPFLAADAT
                     GDCATVTLAPLPSPRPSRKAARTVFDTGSVWRLEETHAGHLLTLRSPVYGQNPYLAVE
                     FDRHYAVGAGETVDGGPHLSGDTMHPFVYPFDEVVYLARLARGRGVLVHACGLVHRGR
                     GILLLGTSGAGKSTSARLWADRPGTTLLSDDRIVIRSDGPRPRIFGTPWHGEAGIESP
                     GSAPLHAVYILEQAPRNRVIDMPPSAAVAQMMVRSFPALWEQEGLQFAVQFLSQLAEQ
                     VPVRRLQFLPEPSAIDCLLASLEPHGTDAGAAA"
     gene            2765..4954
                     /locus_tag="VFD06_04360"
     CDS             2765..4954
                     /locus_tag="VFD06_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02799.1"
                     /translation="MSDAPLPDAVLLAERQGVVFVAAPHGMGPLRRPRRMPPDGAQLV
                     VIPATEDEMRAAELQTAADPDAWPLALPRADLLAPALSRLLHGGHAEMTLLRGRDGVK
                     GILVGADGQKHPFPVATRDALGLLAAMFRCAPRGVVALPGPPSAARLLLSVRPGPRLH
                     EFRLRIAGTVGPPPRSLSDLGLSASLLEVVTDALDQHTGLLGVSGGPASGRGTTLDRM
                     VQALLARGRRGGRIGGRGPAGPLPWIADTLTDWPFPESLREVAPDFLLVDHLEGEREI
                     GLAGRVAACGALVLAGLPPGDPERRARRLRRILEEEARPGVPVGVLSQGLVRTVCRGC
                     RSTTTLPPERALRIGFHRRDVEEMDRHGGLPVARGAGCAACAGTGADGLTGVFAWSGP
                     DDRDTTLPSLQEEGWRRVVDGVVHFEDVMTLPAEGMRAMRSLREVAALAGAPRPPRAA
                     GAAEAQAPAAPLAVDGRAAAESPASPIASAEEDATRLEEIWRAAARGDDRSREIQEMA
                     RALAGRTVDGVLPPLLARGGPSPGPARHAVNTALIASRLSSALGSGEDAPATAFLALV
                     HETGLDERGLDHLGAGGAEMRARLAEVRLLLDAVAPSTERSRSDLRLQAVALAALAHR
                     MHESGLARGLDLHDVTSLVMAQHGQRFSPLLFRALLRAVPIFPIGCLVELSSGDVARV
                     VTQNEENHFRPRVEIASGAPGAERRVVDLARAPFLHIRHRVAAAERA"
     gene            4951..6411
                     /locus_tag="VFD06_04365"
     CDS             4951..6411
                     /locus_tag="VFD06_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HZN02800.1"
                     /translation="MSAARLAATGDATPVAAATIEAEPVLAALLEGGLDVSLPVSGRS
                     MLPTLRPGDRIIVAPCLGLPRPGAILVRRDPAAGFVAHRLVLVEMVDGRRLYRMQGDH
                     EAGLDAGCRREDLMGRVTAVVRDGALLPIDDSPLARRRALFRLAIRRRRPGRWLRRAA
                     ALPVLVSALSAAGAPQARAAAIGPFAQAQTTFAPAADYLFAPGDVLSLRIWDGEKIDE
                     QQLTVQSDGTAFLPILGLGSIAVGGKTAIDAKKEIEDRLGKIYKEIYTELLVLRYAGH
                     RVHLMGEVRTTARTDSGPGDWALEGPTRLVSFLAEHGGPTPEADLMRVQIVRKDGTKE
                     DVNLFRAVFHHRDEDNPILQDGDFVFIPSLQMGLRKVYVLGEVNQPGVVTIFDSMTLI
                     EALARAGGLNQKANPKEVVLIRRRADGKPDLRSANLKELFKTGDLKDDFDIEPGDIVY
                     VSKGFLAHLHDVFQIIAPALDTIESLYIIDDFKNGD"
     gene            6413..8593
                     /locus_tag="VFD06_04370"
     CDS             6413..8593
                     /locus_tag="VFD06_04370"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZN02801.1"
                     /translation="MSAGENLNLQDYLRILRRRAKVVAGCCLLLGLLTFVLTPSAQTT
                     YEAAARVKWTRTDSVAGLFLETFSWSPGDDLATQAQIIASKPVALQAAQRLGRIEEGV
                     TLPEVLSDRTLSARLDAVIASYGARPIENTSLIEIRSASGKPEEAVAIANAVMQEYIT
                     LHTLERNRQAIEARQFVEGQVAEVGGRLAESEDRLAAFKEENVGAKNPDAKEVQFFQD
                     EAGRSEARIVALETLLQSLASGAEGGSPDLLLVELEEDGLQALRQEILKLQDRRHQLE
                     TFMRADAPEVAKVGEQIARLFERLRREARTALDLSQARKAILDAKLSRFPRTEQRLLQ
                     LQRDVQVNAEAYEMLMRKRQEALIREADKVTELSVVETATRAEARQAPGRMLRALVGL
                     LVGLLLGLVAAFAVESLDTSIGSIEDVEQYLGLSVIGVVPHIDADAVRRDILHRQPAL
                     ESDPGLDGLTSLVTQFAPQSPVAEAFRSLRTNLEFSRLGKAGRTFLFTSASPGEGKST
                     TVANLGVAMAQMGMRTLVWDCDLRSPTLNRMFGVAREPGFTHVVLGMARLDDVLHRFS
                     DVFLGRIDMKALQTSAGLENLHVLTAGALPPNPSELLSSPLFNDFVVEVASRFDVILI
                     DAPPVLPVTDAAILSTRVDGVVLVYQLGRISRAALKRAKVHLDNVRATIRGIVLNDVR
                     AEVSSYTPEMAYLNAAYEPEARRGRPAAAGRGAARAVAAFGRRG"
     gene            8610..10007
                     /locus_tag="VFD06_04375"
     CDS             8610..10007
                     /locus_tag="VFD06_04375"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN02802.1"
                     /translation="MPVPRPSFGRFLPALLLVPFGVGLALYIGRAPLAQPLFAAAALV
                     LFAAAFLHAEAGLVLLVASMLLSPEIPLGAAGAGGLEASRSVILRTEDLVLLLVGLGW
                     LARMAIHKDLAAIRRSRLNAAIALYVACCVGSTLLGIEAGRVKPLVGLCYVAKYVEYF
                     FIFFMTLNHVRTPERLQRLLGALLATAAIIVAYAWWQIPGGVRPSAPFEGTPGEPNTL
                     GGYLVLVTAVAAAMALHAPQPVWRRVTAALALAAVPPLIVTLSRSSWLGLAAAIAALI
                     VLSERRGRLAAATIVAGAVLFLAMPQRVEERIRYTFTPEGADAVQVGRVHLDASSSAR
                     LRSWQEALAGFERHPVFGWGITGYGFLDAQYFRVLVEIGSVGALAFATLLFGCGRLFL
                     AARREAADPLHRALGLGLFAGFAGLLAHAIGTNTFLLIRIMEPFWMFTALAAASLALP
                     AAGAPRAVPAARLAA"
     gene            10004..11599
                     /locus_tag="VFD06_04380"
     CDS             10004..11599
                     /locus_tag="VFD06_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZN02803.1"
                     /translation="MSHVPIGPGPLPSPGADERRAAARGVAWSGVEAAAAALTALLMT
                     PLVLRTCGLEGLGLWGAAWSVAHTAGLIDLGVGSAYGRFAAQALARGDERDLNATLAV
                     GVGFQGAVSLVILGLAALFGPALLDRVLPAGDRPPEAGFVLGATFLTVLLRGTLSAYR
                     GVVAGAQRLDLLARIGTVGTIGEAALAAALLLSGGGLRGPAIAALVGALGVTLAEAAA
                     AHRLCPGLHIYPFRAPREQYARVLVFGGQVQVTRAFEVLGGHAPRLLLAAGPGLAAAG
                     AYEIAARLSGFAQSAASLPLKVLIPLAGHLEVRADTRRLKALLERSTRYVTLLALPVV
                     VALLLLPETVIVAWTARPAPPGTAAAARLVVLAVAALVVVSPLRLLLRASGRAGLEAA
                     ATAAGALVQIGAAAALAGRHGAPGVALATCAGAIVALLFVAAGGRRAATLRTGDAARA
                     ARAPLIAGLGALAVGIALQMLFPVAAATGRGQAIATLLTHLLLPAAVFLGLAWRGRAA
                     GRDEIGLLFEALRAPSRAAGRAS"
     gene            11596..12648
                     /locus_tag="VFD06_04385"
     CDS             11596..12648
                     /locus_tag="VFD06_04385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN02804.1"
                     /translation="MRVLLSGAFHPDFEALPDYLAAALARQGHDVALFDHRRFLLPGR
                     LRARAPLLHRLDRARLNRAFAAAVRRHRAAVAIVNQGMTIEPATIARLRDAGVRMVNW
                     FSDFPAEFEEGLVAAPAYDAFHLGSSYAVERHRALGHRHASWLPFACDPQAHRPEGPM
                     DVIGPGRVVFAGSHYPERQVLLRHLRGLPLDVYGPGWERARDDGHVAPMIRDPFALRP
                     AAWRRLYSRAAAVLNIHYGAFGPVSASGEMANTRAFEIPACGALQIADRRRDLLAVFR
                     EGEELLAFSSGEELRCVVETALADEALRRNVAAAGRRAVLAGHTYDDRVRVLLGEAQA
                     PALPGAADGMAQGRAA"
     gene            12645..13757
                     /locus_tag="VFD06_04390"
     CDS             12645..13757
                     /locus_tag="VFD06_04390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN02805.1"
                     /translation="MSRARVLHVVEALGLGGLEQVVLSLTRHASPDFPPEVLALSRGG
                     PVQAAIEAAGVRVRRLALNDYYPGSVLRAARAVRAASPDVIHTHGHFAGVAGRLAARL
                     AGVRALVHHLHTADSTLKPRHRRLERLLARGTRRIVCCSEAVVRHARVDLGAPEDLAV
                     VVRNGIDPPAAISREAARLLLPPELTEPIVGCVGGLWPHKGQAVLLEAWGALPPSLRR
                     GSLLFAGDGSERAALEEQATRLGVADAVRFLGLRPDVRSFLPALALLVAPSIGREGLG
                     LAVLEGMDAGLPVIASRTGGLPEVVEEGATGLLVPERDPAALQAAMAALLADEGRRLR
                     MGDAGRRRVEREFRAEPMTRRIEAIYEAAIGVQRAA"
     gene            13765..14922
                     /locus_tag="VFD06_04395"
     CDS             13765..14922
                     /locus_tag="VFD06_04395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN02806.1"
                     /translation="MVLLVSERADFFGGGQRSLLDLAAALQAGARFAPLVVLPEEGPL
                     GAALAAAGVVVRRLPLPAVRGGALFATPRALADLARLAREAGAALLHSDSPRTALYAG
                     TAARLLGRPHLWHVRASVGSSAFADAALLALSDRVVAVSRAAARRSPPLARSSRLSVI
                     PTGIAPPVCLPRREARLLLGLEPEGYVVGVVGRVEPDKGGEEAVASLPLLRLARPEAR
                     LVFLGDTGSPGVLVGDDAGSHAERLGEIARSLGLGDAVRFAGPRDAAAPLLSAFDLLI
                     HPSRHEALPRVVLESLHAGVPVVATAVGGVPEAIEDGVCGLLVPPRDPEQLGAASARL
                     ASDADFAARLVSAGRERARTRFSLAAMTEALEDLYDRMTSRVARRAQRGRR"
     gene            15039..15782
                     /locus_tag="VFD06_04400"
     CDS             15039..15782
                     /locus_tag="VFD06_04400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN02807.1"
                     /translation="MCVACGLIFLDPVPDEATETGAYGSDYYAPWQGEEERPRRRMWR
                     TRLRAIDRHAGRGSLLDIGCGDGLFLEEARRAGWRGDGIEFSPEGARRAAARLGRPVA
                     VGDLAKSKVLPGPYDVATLWHVVEHLPEPRPMIEAARRRLRRGGLLVVAVPNTDNLPM
                     RAAYRLGRGRRLPLWEPGAREPHVSHFTPATLRALLLRGGFDPLVIEPDRCALTPAKR
                     LIDAVAASLSILGGVLVTDAIAVFARRPA"
     gene            15779..16939
                     /locus_tag="VFD06_04405"
     CDS             15779..16939
                     /locus_tag="VFD06_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZN02808.1"
                     /translation="MNPPVPFPLKPVTVPPDRHTAERLRAAARQVVLAARTPVLLAAR
                     LVRRRVTPPAVTLVHRLLVIRTDRIGDMVLSTAALADLKAHFRHARITVLAPEGPLQV
                     LRGHPAVDALVPLKDRRLPPDLMGKFDLAIDLTPDARLLGARLAAATGAPWRTGLSAY
                     GRQAFFTLPTAKARPDLHIVDLNRDLLRAIGVPSPAAEPTLHLQPSERAAAQARVSAL
                     GAAAPRVVVHPGAHYPSQRWAAGRFAEVIARLTETVGAACIVVAGPGEEDLAEAIGAA
                     TPDALLPGPLDVRGLMGMIAVADLFLGNNSGPLHIAAALGVPTLSVMGPTDPRRFAPR
                     RPEDTVLRLELPCSPCQRARCWHHTCLEAIPAEVVAARARDILRAEPAEQAA"
     gene            16936..18093
                     /locus_tag="VFD06_04410"
     CDS             16936..18093
                     /locus_tag="VFD06_04410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN02809.1"
                     /translation="MSRSLSVLHLITRLERGGASDCTLWQAIGAARRGHRVTLACGPS
                     EIETPLIARAHRQAGLEILTLPALGRTLRPGRDLRALAAIATLIKSRPFDVIHLHTSK
                     AGALGRIAAGLLGQSQRVVHQPHGHLFYGYYGRMGGRMVLLAERLLAPLARRHVTLTG
                     RGAREHLARGVGQADQFHVIPSGVDFRPLRKAARQHGAARQRLGYAASEFVVMSLARL
                     EPVKGAEETLEAFAAAARSRPDLRLHLGGDGPLLESLRARARALGIADRVRIDGVWAA
                     PEDLLPAADLFVLAPRNEGMGRAVIEALALSLPVIATAVGGLPEVLEDGAAGILVPPR
                     DTAALSEAIGRIADDPALARAFGRRGRARAVEFGAGRMVHRVLKLYRDIAA"
     gene            18161..18856
                     /locus_tag="VFD06_04415"
     CDS             18161..18856
                     /locus_tag="VFD06_04415"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HZN02810.1"
                     /translation="MILAAHQPQYLPWLGYFDKMARADRFVLIDTVQYKKNEWQNRNR
                     IRAASGWQWLTVPVHARFPARIDEVAADEAAPWRRKHAATLRQAYARAPHRDAVLPSL
                     ERLLERPLHRLAEINIESIRLLADLLEVRTETILASDIEGIPDGPDARLIALCRRFGA
                     DRYLSGAGGRDYMDLDAWKRAGIEVVFQEFRGPVYAQGRSGFEPNLSAVDFLVHCGPG
                     PIERPAAPARVAA"
BASE COUNT         2149 a         7208 c         6880 g         2671 t
ORIGIN      
        1 cgccctgccg cggcgcggcg tggcgatcct ggaggacgcg gcgcgccggc gccgcgcggg
       61 gacgcgatga cgccgggcgc cctcgccggg gcctcgatcg tcgtgccggc gccggacgtc
      121 gtggcgcgcg acatcgccgg cgagcacctg ctcgtcccgg tccgctccgg gatcgccggt
      181 gtcgattcca tttacaccgc cgacgaggtc ggctcgttcc tctacgcgcg cctcgacggg
      241 cggcgctccg cggcggagct cgcgcgcctc gtctccgagg cctacgtcgt cccggaggac
      301 cgcgccctcg ccgacgtcct ggcgttcctg cgcgagctgt gcgacgccgg gctggcgcgg
      361 cccgcggaga cccggtgagc ggcggcaccg cgaccttcaa cgccttcggc gcgcgcgtcc
      421 tcgaggcatg cggcggggag cgcgtgccgc tgaacggctc gctcgagctc acctttcgct
      481 gcaacctgcg ctgtgtgcac tgctacatcc ccgatttctc cggccgcggc gagatgacga
      541 cgtcggagat cgtccgcatc ctcgacgaga ccgccgacgc cgggtgcctg tggatgctcc
      601 tcaccggcgg cgaggtcctg agccgtcccg acttcgcgga gatctaccgg cacgtcaagc
      661 ggcgcggcat gctcccgacg gtcttcacca acgggacgct cctgcaggat cgtcacgcgg
      721 atctcttcgc cgagtacccg ccgttcagcc tcgagatcac cctctacggg atgtcggacg
      781 ccacctacga gcggaccacc gggatgccgg ggggcttccg ccgggtgcgc gcggccgtgg
      841 agcggctcgt cgagcgccgg gtccggctga ccctcaaggc ggtggcgatg gcgccgctcg
      901 tcggcgagct gccggccatg cgcgcctggg ccgacctcct cggggttccc ttccgctacg
      961 acgccaccat ccacgggagg ctcgacggct cgctgcaacc ggccgccgcc cgggccacgc
     1021 cggccgaggt ggtcggcctc gacgccgccg acgccgagcg gcgcgtccac tggcagcgct
     1081 tctacgacca gttcgtgaag aacgctccga agaccgaccg gctcctgtcg tgcggcgccg
     1141 gcctgcagtc gttccatgtc gatccgaagg gctggctgtg gtcgtgcgag gcgctgccgc
     1201 tcgacggcta cgacctgcgc cacggtactt tcctcgaagg ctggaacggc atcgtcggag
     1261 aggtgcggca ccgccgcgcg tcggccgcga acgtctgcgc cggctgcgac ctcaagtcgc
     1321 tgtgcgatcg ctgccccgcg accgcgatcc tcgagaccgg cagcccggac gggtggatcc
     1381 cccactactg cgagatcacc caccgccgcg ccgcgctcct ggagtccgag tccggagatc
     1441 cggagcgggc cgcccgctac ctcgcccacg ccgagaaggt cgccgcgggc tggacgccgc
     1501 cgggagtgac gctgccgcgc gccagccggc tgccgcaggc gccttcctgc ggaaccggct
     1561 gcggcgccgc ctgcggccgg gagtcctccg gcgcgcggcc cgtgcgatcc gccctcctga
     1621 gcatcgagcg ccccgaaaca tcccgggaga tcctccgatg agcccgaaac ccgacacgcc
     1681 cgacgcctct ccgcgccggg cgtaccagcc gccgcgtctg acccgggtgc aactgatccc
     1741 ggacgaggcg gtgctggcct cctgtaagaa cgcctccact cagggaccgc tcggcgggtc
     1801 ccgaaaatgc accggacccc cggcgctctg ttcccacagt ggctcctgac gcgttccgcc
     1861 cgctggtcct cgaaatcgcc ggcctcggtc tcgaagtgaa gccggcggag ccggggatcc
     1921 gcctcgtcac cgacgcggcg caccagccgt tcctggccgc cgacgccacg ggggactgcg
     1981 ccacggtgac gctggcgccg ctcccctcgc cgcgtccgtc gcggaaggcg gcgcgcaccg
     2041 tcttcgacac cggatcggtg tggcgcctgg aggagaccca tgccgggcac ctgctgaccc
     2101 tgcgctcccc cgtgtacggg cagaatccct acctcgcggt ggagttcgac cgccactacg
     2161 ccgtcggggc cggcgagacg gtggacggcg gcccgcacct ctccggcgac accatgcacc
     2221 cgttcgtcta tcccttcgac gaagtggtgt acctggcgcg cctggcgcgc ggccgcggcg
     2281 tgctggtgca cgcctgcggc ctcgtgcacc gggggcgcgg cattctcctc ctggggacga
     2341 gcggcgccgg caagagcacc tcggcgcggc tgtgggcgga ccgccccgga acgaccctgc
     2401 tgtccgacga ccggatcgtc atccgctcgg acgggccgcg gccgcgcatc ttcgggaccc
     2461 cgtggcacgg cgaggcggga atcgaatccc ccggatcggc gccgctgcac gccgtctaca
     2521 tcctcgagca ggcgccgcgc aaccgggtga tcgacatgcc gccatcggcg gcggtggcgc
     2581 agatgatggt gcgctccttc cccgcgctgt gggagcagga gggcctgcag ttcgccgtgc
     2641 agttcctgtc gcagctcgcc gagcaggtcc cggtgcggcg actgcagttc ctgccggagc
     2701 cctcggcgat cgactgcctg ctcgcctcgc tcgaacctca cgggacggac gcgggggcgg
     2761 cggcgtgagc gacgctccgc tcccggacgc cgtcctcctg gccgagcgcc agggggtggt
     2821 gttcgtcgcc gccccgcacg ggatgggccc gctgcggcga ccgcgccgga tgccgccgga
     2881 cggcgcgcag ctcgtcgtca ttccggcgac cgaggacgag atgcgggccg ccgagctgca
     2941 gaccgccgcg gacccggacg cctggccgct ggccctcccg cgcgccgatc tcctggcgcc
     3001 ggcgctgtcg cgcctgctcc acggcggcca cgccgagatg accctcctgc gcggacggga
     3061 cggcgtgaag gggatcctgg tcggcgcgga cggacagaag cacccgtttc cggtggcgac
     3121 gcgcgacgcg ctcggtctgc tcgcggcgat gttccgctgc gcgccgcgcg gcgttgtggc
     3181 gctgcccggg ccgccttcgg cggcgcgcct gctgctgtcg gtgcgccccg gtccgcgcct
     3241 gcacgagttc cgcctgcgca tcgccggcac cgtcgggccg ccgccgcgat cgctctccga
     3301 tctcgggctc tccgcctcgc tcctggaggt cgtcaccgat gcgctggacc agcacaccgg
     3361 cctcctgggg gtctcgggcg gtccggcgag cggacgcggc accacgctcg atcggatggt
     3421 gcaggcgctc ctggcgcgcg gccgccgcgg cggccggatc ggcgggcgcg gccccgccgg
     3481 ccctctgccg tggatcgccg acacgctcac cgactggccg tttccggaga gcctgcggga
     3541 ggtcgccccc gacttcctcc tcgtcgatca cctcgagggg gagcgggaga tcgggctcgc
     3601 cgggcgcgtc gccgcctgcg gcgcgctcgt cctcgccggt ctgccgcccg gcgacccgga
     3661 gcggcgcgcg cgacggctgc gccggatcct cgaagaggag gcgcgtcccg gggtgccggt
     3721 gggggtcctg tcgcaggggc tggtgcgcac cgtctgccgc ggctgtcgtt cgacgacgac
     3781 gctcccgccg gagcgggcgc tccggatcgg cttccaccgc cgcgacgtgg aagagatgga
     3841 tcgccacggc gggctcccgg tggcccgcgg cgccggctgc gccgcctgcg ccggcaccgg
     3901 ggcggacggg ctgaccgggg tgttcgcgtg gtccggcccg gacgatcggg acaccaccct
     3961 cccgagcctc caggaggaag gatggcgccg ggtggtcgac ggggtcgtcc acttcgagga
     4021 cgtcatgact cttccagccg aggggatgcg ggcgatgcgc tcgctgcgcg aagtggcggc
     4081 gctggccggg gcgccgcggc cgccgcgcgc cgccggcgcg gccgaggcac aagcccctgc
     4141 ggcaccgctg gcggtggacg gacgcgccgc ggccgaatcc cccgcctcgc cgatcgcctc
     4201 ggccgaggag gacgcgaccc gactggagga gatctggcgc gccgccgcgc gtggcgacga
     4261 ccgatcgcgc gagatccagg agatggcacg cgccctggcg gggcgcaccg tggacggggt
     4321 gctgccgccg cttctggcgc gcggcgggcc gtcccccggc ccggcgcggc acgcggtcaa
     4381 cacggccctc atcgcgtcgc ggctctcctc cgccctgggc tccggcgagg acgcgccggc
     4441 gacggcgttc ctggcgctgg ttcacgagac cgggctcgac gagcgcgggc tcgatcatct
     4501 cggagccgga ggggcggaga tgcgcgcgcg cctcgcggag gtgcgcctcc tgctcgacgc
     4561 ggtcgcgccg tcgaccgagc ggagccgctc cgatctgcga ctgcaggcgg tcgccctcgc
     4621 ggcgctggcg caccggatgc acgagtccgg gctggcgcgc ggcctggatc tccacgacgt
     4681 cacctcgctg gtcatggcgc agcacgggca gcgcttctcg ccgctcctgt tccgggctct
     4741 tctccgcgcc gtgccgatct tcccgatcgg ctgcctcgtc gagctgtcga gcggggacgt
     4801 ggcgcgcgtc gtgacgcaga acgaggagaa ccacttccgg ccgcgcgtcg agatcgcctc
     4861 gggcgcgccg ggagcggagc ggcgggtggt cgacctggcg cgcgcgccgt tcctgcacat
     4921 ccgccaccgg gtcgccgcgg cggagcgcgc gtgagcgcgg cccgcctcgc ggccaccggc
     4981 gacgccacgc cggtcgccgc cgccacgatc gaggcggagc cggtgctggc ggcactcctc
     5041 gagggggggc tcgacgtctc cctgccggtg agcggccgga gcatgctgcc gacgcttcgt
     5101 cccggggatc ggatcatcgt cgccccctgc ctcggtctcc cccgccctgg cgcgatcctg
     5161 gtccggcggg accctgcggc cgggttcgtg gcgcaccgcc tcgtcctggt ggagatggtg
     5221 gacgggcggc gactctaccg catgcagggg gaccatgaag ccgggctcga cgccgggtgc
     5281 cggcgcgagg acctcatggg gcgcgtcacc gccgtggtgc gcgacggggc cctcctgccg
     5341 atcgacgact ctcccctggc gcgccgtcgg gcgctgttcc gcctggcgat ccgccggcgg
     5401 cggcccggcc ggtggctgcg gcgcgccgcg gcgcttcccg tcctcgtctc cgccctgagt
     5461 gcggccggcg cgccgcaggc gcgcgccgcg gcgatcggtc ccttcgccca ggcgcagacg
     5521 accttcgccc cggccgccga ctacctcttc gcgcccgggg acgtcctgtc gctgcgcatc
     5581 tgggacggcg agaagatcga cgagcagcag ctcacggtgc agagtgacgg caccgccttc
     5641 ctgccgatcc tcgggctcgg ctcgatcgcc gtcggaggca agaccgccat cgatgcgaag
     5701 aaggagatcg aggatcgcct cgggaagatc tacaaggaga tctacaccga gctcctcgtc
     5761 ctgaggtacg cgggccaccg cgtccacctc atgggggagg tgcgcacgac ggcgcgcacc
     5821 gacagcggcc cgggcgactg ggcgctcgag gggccgacgc ggctcgtctc cttcctcgca
     5881 gagcacggcg gaccgacccc ggaggccgat ctcatgcggg tgcagatcgt ccgcaaggac
     5941 ggcacgaagg aggacgtcaa cctcttccgg gcggtcttcc accaccgcga cgaggacaac
     6001 ccgatcctgc aggacgggga cttcgtcttc atcccctcgc tgcagatggg gctgcgcaag
     6061 gtatacgtcc tgggggaggt gaaccagccc ggcgtcgtga cgatcttcga cagcatgacc
     6121 ctgatcgagg cgctggcgcg ggccggcggg ctgaaccaga aggccaaccc caaagaggtc
     6181 gtcctcatca ggaggcgcgc cgacggcaag ccggatctcc gatcggcgaa cctgaaggaa
     6241 ctgttcaaga ccggcgatct caaggacgac ttcgacatcg agccggggga catcgtctac
     6301 gtctcgaagg gcttcctcgc ccacctgcac gacgtcttcc agatcatcgc gccggccctc
     6361 gacaccatcg agtcgctcta catcatcgac gacttcaaga acggggactg acgtgagcgc
     6421 gggcgagaac ctgaacctgc aggactacct gcgcatcctg cgccgccgcg ccaaggtggt
     6481 ggcgggctgc tgcctgctgc tcggtctgct gacgttcgtc ctgacgccga gcgcgcagac
     6541 cacctacgag gcggcggcgc gtgtgaagtg gacccgcacc gacagcgtcg ccgggctctt
     6601 cctcgagacc ttctcgtgga gccccgggga cgacctcgcc acgcaggcgc agatcatcgc
     6661 cagcaagccg gtcgcgctgc aggcggcgca gcgcctcggg cgcatcgagg agggggtgac
     6721 gctgccggag gtgctgtccg atcggacgct ctcggcgcgc ctcgacgcgg tgatcgcctc
     6781 ctacggggcg cggccgatcg agaacaccag cctcatcgag atccgctccg catccggcaa
     6841 gccggaggag gcggtggcga tcgccaacgc cgtgatgcag gagtacatca ccctgcacac
     6901 cctcgagcgc aaccggcagg cgatcgaggc ccggcagttc gtcgaggggc aggtggcgga
     6961 ggtgggaggc cgcctcgccg agtcggagga tcggctcgcc gccttcaagg aggagaacgt
     7021 cggggcgaag aacccggacg ccaaggaggt gcagttcttc caggacgagg cggggcggag
     7081 cgaggcgcgc atcgtcgcgc tcgagacgct gctccagagc ctcgcgtccg gggcggaggg
     7141 cggcagcccg gatctcctgc tcgtcgagct ggaggaggac gggctgcagg cgctgcggca
     7201 ggagatcctg aagctccagg accggcggca ccagctggag accttcatgc gcgccgacgc
     7261 gccggaggtc gcgaaggtcg gggaacagat cgcgcgtctc ttcgagcggc tgcggcgcga
     7321 ggcgcgcacc gcgctcgacc tgtcgcaggc gcgcaaggcg atcctcgacg ccaagctgtc
     7381 ccgcttcccg cgcaccgagc agcggctcct ccagctgcag cgcgacgtac aggtcaacgc
     7441 cgaggcgtac gagatgctga tgcgcaagcg ccaggaggcg ctcatccgcg aggcggacaa
     7501 ggtcaccgag ctgtcggtgg tcgagaccgc cacgcgggcc gaggcgcgcc aggcgcccgg
     7561 ccggatgctg cgcgcgctcg tcgggctcct ggtcggcctg ctgctcggcc tcgtcgccgc
     7621 gttcgccgtg gagagcctcg acacctccat cggcagcatc gaggacgtcg agcagtacct
     7681 cggcctgtcg gtcatcggcg tcgtgccgca catcgacgcc gacgccgtgc gacgcgacat
     7741 cctgcaccgc cagccggcgc tcgaatccga tccggggctc gacgggttga cctcgctcgt
     7801 gacccagttc gcgccgcagt cgccggtcgc cgaggcgttc cgatcgctgc gcaccaacct
     7861 cgagttctcg cgcctcggca aggcggggcg cacgttcctg ttcacctccg cctcgcccgg
     7921 ggaggggaaa tcgacgaccg tggccaacct gggcgtggcg atggcgcaga tggggatgcg
     7981 gacgctcgtc tgggactgcg acctccgcag ccccaccctc aaccgcatgt ttggcgtggc
     8041 gcgcgagccc ggcttcaccc acgtcgtcct cgggatggcg cggctcgacg acgtcctgca
     8101 ccgcttctcc gacgtcttcc tcgggcgcat cgacatgaag gcgctgcaga cctccgccgg
     8161 cctcgagaac ctgcacgtgc tgaccgccgg ggcgctgccg ccgaacccgt cggagctgct
     8221 gtcgtcgccg ctgttcaacg acttcgtggt cgaggtcgcg tcgcgcttcg acgtcattct
     8281 gatcgacgct ccgccggtgc tgccggtgac cgacgccgcc atcctgtcga cgcgcgtgga
     8341 cggggtggtg ctggtgtacc agctgggccg gatcagccgg gcggccctga agcgggccaa
     8401 ggtgcacctc gacaacgtgc gcgcgaccat ccgcgggatc gtgctcaacg acgtccgtgc
     8461 ggaggtctcc tcctacaccc ccgagatggc ctacctgaac gcggcctacg agccggaggc
     8521 gcgccgcggg cgtccggccg ccgccggccg aggagcggcg agggccgtgg ccgccttcgg
     8581 gaggcgcggc taggagcgag gagcccagga tgcccgtccc ccgtccttcc ttcggccgct
     8641 tcctcccggc gctcctgctc gtcccgttcg gcgtcggcct ggcgctgtac atcggccgcg
     8701 cgccgctggc gcagccgctg ttcgccgcgg ccgcgctcgt gctgttcgcc gccgcgttcc
     8761 tgcacgccga ggcgggactc gtcctcctcg tcgcctcgat gctcctctcc cccgagatcc
     8821 cgctcggggc ggcgggggcc ggcgggctcg aggcctcgcg cagcgtcatc ctgcgcaccg
     8881 aggacctggt gctcctgctc gtcggcctcg gctggctggc gcgcatggcg atccacaagg
     8941 acctcgcggc gatccggcgc tcgcggctca acgccgccat cgccctctac gtcgcctgct
     9001 gtgtcggttc gaccctcctc ggcatcgagg cggggcgcgt gaagccgctc gtcggcctgt
     9061 gctacgtcgc gaagtacgtc gagtacttct tcatcttctt catgacgctg aaccacgtgc
     9121 gcacccccga gcgtttgcag cgcctcctgg gcgcgctcct cgcgacggcg gcgatcatcg
     9181 tggcctacgc ctggtggcag atccccggtg gcgtccgccc ctcggcgccg ttcgaaggga
     9241 cgcccgggga gccgaacacg ctcggcggct acctcgtgct cgtgacggcg gtcgccgccg
     9301 ccatggcgct gcacgcgccg cagcctgtct ggcgccgcgt cacggcggcg ctcgccctcg
     9361 cggcggtccc gcccctcatc gtgacgctgt cgcggtcctc ctggctcggg ctcgccgcgg
     9421 cgatcgccgc cctgatcgtc ctctcggagc gtcggggccg gctggcggcc gcgacgattg
     9481 tcgccggcgc ggttctgttc ctggcgatgc cgcagcgggt cgaggagcga atccgctaca
     9541 ccttcacgcc ggaaggggcc gacgcggtgc aggtgggccg cgtgcacctc gacgcctcgt
     9601 cgtcggcgcg gctgcgctcc tggcaggagg cgctcgccgg cttcgagcgg catccggtct
     9661 tcggctgggg gatcaccggc tacggctttc tcgacgcgca gtacttcagg gtgctcgtcg
     9721 agatcggcag cgtcggagcc ctggcgttcg cgacgcttct gttcgggtgc gggaggctct
     9781 tcctggcggc gcgccgcgag gccgcggatc ccctgcaccg cgccctgggg ctcgggctct
     9841 tcgccgggtt cgcggggctc ctggcgcacg ccatcggcac caacacgttc ctgctcatcc
     9901 gcatcatgga gccgttctgg atgttcaccg ccctcgccgc cgcgtcgctc gccctgccgg
     9961 cggccggggc gccgcgcgcc gtcccggccg cgaggctcgc ggcatgagcc acgttccgat
    10021 cggacccggc ccgcttccct cccccggcgc cgacgagcgg cgcgcggcgg cccgcggcgt
    10081 cgcgtggagc ggggtcgagg cggccgccgc ggcgctgacg gccctcctca tgaccccgct
    10141 ggtcctgcgc acctgcggcc tcgaggggct cgggttgtgg ggggccgcgt ggtcggtggc
    10201 gcacaccgcc ggcctcatcg acctcggggt gggctcggcc tacggccgct tcgccgcgca
    10261 ggcgctggcc cgcggcgacg agcgcgatct caacgccacg ctcgccgtcg gcgtcggatt
    10321 ccagggtgcg gtgtcgctcg tcatcctggg gctcgccgct ctcttcgggc cggcgctcct
    10381 cgaccgcgtc cttcctgccg gcgatcgccc ccccgaagcc ggcttcgtgc tcggcgcgac
    10441 gttcctgacg gtgctcctac ggggcaccct ctccgcctac cgcggcgtgg tcgcgggcgc
    10501 gcagcgcctc gatctgctcg cccgcatcgg aacggtcggg acgatcggcg aggcggcgct
    10561 ggcggcggcc ctgctgctgt cgggaggcgg cctgcgcggg ccggcgatcg ccgcgctcgt
    10621 cggggcgctc ggcgtgacct tggcggaagc ggcggcggcc caccgcctct gccccgggct
    10681 tcacatctac ccgttccgcg ccccgcgcga gcagtacgcg cgcgtcctgg tgttcggagg
    10741 gcaggtgcag gtgacgcgcg ccttcgaggt cctcggcgga cacgccccgc gcctcctcct
    10801 cgcggccggc ccgggactgg cggcggcagg ggcctatgag atcgccgcgc gcctctccgg
    10861 cttcgcgcag tcggcggcgt ccctgccgct caaggtcctg atccccctgg ccgggcacct
    10921 cgaggtccgc gccgacacgc ggcggctcaa ggcgctgctc gagcgctcga cgcgctacgt
    10981 gacgctcctc gcgctgccgg tggtcgtcgc gctcctgctc ctgccggaga cggtgatcgt
    11041 ggcgtggacc gcccggcccg ccccgcccgg caccgcggcc gcggcgcgcc tcgtggtcct
    11101 cgccgtggcg gcgctcgtcg tcgtctcccc cctacggctg ctcctgcgcg ccagtggccg
    11161 cgccgggctg gaggccgccg cgaccgcggc cggagcgctc gtccagatcg gcgcggcggc
    11221 ggccctcgcg gggcgccatg gcgcgcccgg agtcgcgctc gcgacctgcg cgggtgcgat
    11281 cgtggcgctt ctgttcgtcg ccgccggcgg ccgccgggcg gcgacgctcc ggaccggcga
    11341 cgccgcgcgt gccgcgcgag cgccgctcat cgccgggctc ggcgccctcg cggtggggat
    11401 cgcgctgcag atgctcttcc ccgtggccgc cgccaccggg cgcgggcagg cgatcgccac
    11461 cctcctgacg cacctcctcc tgccggcggc cgtcttcctc gggctggcgt ggcggggacg
    11521 ggccgccggc cgggacgaga tcgggctgct cttcgaggcg ctgcgggccc cctcccgcgc
    11581 ggccgggagg gcgtcgtgag ggtgctcctg tccggcgcct tccacccgga cttcgaggcg
    11641 ctccccgact acctcgcggc ggccctcgcg cggcagggac acgacgtcgc gctcttcgat
    11701 caccggcggt tcctcctgcc gggacggctg cgggcgcggg caccgctcct ccaccgcctc
    11761 gaccgcgccc ggctcaaccg cgccttcgcc gcggcggtgc ggcgccaccg ggcggctgtc
    11821 gcgatcgtca accaggggat gacgatcgag cctgcgacca tcgcccgcct gcgcgacgcc
    11881 ggcgtccgca tggtcaactg gttctccgac ttcccggccg agttcgaaga gggactcgtc
    11941 gccgctcccg cctacgacgc cttccacctc ggatcgtcgt acgccgtcga gcgacaccgg
    12001 gcgctcgggc accggcacgc ctcctggctg ccgttcgcct gcgatccgca ggcgcaccgt
    12061 cccgaagggc cgatggatgt catcggtccc ggccgcgtcg tcttcgccgg ctcgcactac
    12121 ccggagcgtc aggtgctgct ccggcacctg cgcggcctcc cgctcgacgt ctacgggccg
    12181 ggctgggagc gggcgcgcga cgacggccac gtcgcgccga tgatccgcga tcccttcgcg
    12241 ctccgcccgg ccgcgtggcg ccgcctctat tcccgcgccg cggccgtcct caacatccac
    12301 tacggggcgt tcggcccggt gagcgcctcc ggcgagatgg cgaacacgcg cgccttcgag
    12361 atcccggcgt gcggggcgct gcagatcgca gaccggcgcc gcgatctgct cgcggtgttc
    12421 cgcgaagggg aggagctcct ggccttctcc agcggcgaag agctgcgctg cgtcgtcgag
    12481 acggcgctcg ccgacgaggc gctgcgccgg aacgtcgcgg cagccggtcg ccgcgccgtc
    12541 ctcgccgggc acacctacga cgatcgcgtc cgggtcctcc tcggcgaggc gcaggcgccg
    12601 gcccttccgg gggcggccga cgggatggcg caggggaggg cggcgtgagc cgggcccggg
    12661 tcctgcacgt cgtcgaggcg ctcgggctcg gcgggctcga gcaggtggtc ctgagcctga
    12721 cgcggcacgc ctcccccgac ttcccgccgg aggtcctggc gctcagccgg ggcggcccgg
    12781 tgcaggccgc gatcgaggcg gccggagtgc gggtgcggcg gctggcgctc aacgattact
    12841 accccgggag cgtgctgcgg gcggcgcgcg ccgtgcgggc cgccagcccc gacgtgatcc
    12901 atacccacgg acatttcgcg ggggtcgccg ggcgcctggc ggcccgtctc gcgggggtgc
    12961 gggcgctcgt ccaccacctg cacaccgcgg actccaccct caagccgcgc caccgccgcc
    13021 tcgagcggct cctggcgcgc ggaacccggc ggatcgtctg ctgctccgag gcggtggtgc
    13081 gccacgcgcg cgtcgatctc ggcgcgccgg aggacctcgc ggtcgtcgtc cggaacggca
    13141 tcgacccgcc cgccgcgatc tcccgcgagg cggcgcgcct cctcctcccg cccgaactca
    13201 cggagccgat cgtcggctgc gtcggcgggc tctggcccca caagggacag gcggtgctcc
    13261 tcgaggcctg gggggcgctg ccgccgtcgc tgcgccgggg ttcgctcctg ttcgccggcg
    13321 acggaagcga gcgcgccgcc ctcgaggagc aggcgacgcg cctcggcgtg gcggatgcgg
    13381 tgcgcttcct cggactgcgc cccgacgtgc ggtcgttcct gccggcactc gcgcttctcg
    13441 tcgctccgtc gatcggccgg gaggggctcg ggctcgcggt gctcgaaggg atggacgcgg
    13501 ggctcccggt catcgccagc cgcaccggcg gcctgccgga ggtggtcgag gagggggcga
    13561 ccgggctgct cgtgcccgag agggatccgg cggcgcttca ggcggcgatg gccgctctcc
    13621 tcgcggacga gggacgacgc ctgaggatgg gcgacgccgg gcgcaggcgc gtggagcggg
    13681 agttccgcgc cgagccgatg acccggcgca tcgaggcgat ctacgaggcg gcgatcgggg
    13741 tgcagcgtgc ggcctgagcg gcccgtggtc ctcctcgtga gcgagcgcgc tgacttcttc
    13801 ggcggcggcc agcggagcct gctcgatctc gccgccgcgc ttcaggccgg cgcccgcttc
    13861 gcgcctctgg tcgtgctgcc ggaggagggt ccgctcggcg cggcgctcgc cgccgccggg
    13921 gtggtggtgc gacggcttcc tctccctgcg gtccggggcg gggcgctgtt cgccacccct
    13981 cgcgccctcg ccgacctcgc gcggctggcg cgggaggccg gggccgcgct cctccacagc
    14041 gactcgccgc gcaccgccct ttacgccggg accgcggcgc gccttctcgg ccgaccgcac
    14101 ctgtggcacg tgcgggcgtc cgtcggctcg tcggcgttcg ccgacgcggc gctcctggca
    14161 ctttcggatc gggtcgtcgc cgtgtcgcgg gcggcggcgc gacggagccc gcccctcgca
    14221 cgctcgtcgc gcctgagcgt catccccacc ggcatcgcgc ctccggtctg tctgccgcgc
    14281 cgggaggcgc gcctcctcct cgggctcgag ccggagggtt acgtcgtcgg tgtcgtcggg
    14341 cgcgtcgagc cggacaaggg gggggaggag gcggtggcgt cgctgcctct cctgaggctc
    14401 gcccgcccgg aggcgcgtct cgtcttcctg ggcgacaccg ggtcacccgg tgtcttggtc
    14461 ggcgacgacg ccggatcgca cgcggagcgg ctcggggaga tcgcccgctc gctcggtctc
    14521 ggcgacgcgg tccgcttcgc cggcccgcgc gacgcggcgg cgccgctgct ttcggcgttc
    14581 gatctgctga ttcatccctc gcgccacgag gcgctgccgc gcgtcgtcct ggaatccctc
    14641 cacgcggggg ttccggtcgt ggcgaccgcg gtcggcggtg tccccgaggc gatcgaggac
    14701 ggcgtgtgcg gcctgctggt gccgccgcgc gacccggagc agctcggcgc cgcctccgcc
    14761 cgcctcgcgt ccgatgcgga cttcgccgcc cggctcgtca gcgccggaag ggagcgcgcc
    14821 cggacccgat tctcgctcgc ggccatgacc gaagccctcg aagatctcta cgaccggatg
    14881 acctccaggg tcgcgcgccg cgcgcagaga ggcagacggt gatcctctcc ggccgggaag
    14941 ccaaaggacc ccgcaccacc cgcccgtgtc cgctctgcga tcatcacacc ggccggctca
    15001 accgcctcac cgcggcctgg gccgaacccc ggcccatcat gtgcgtcgcg tgcgggttga
    15061 tcttcctcga tccggtcccg gacgaggcga ccgaaacggg cgcttacgga tccgactact
    15121 acgccccctg gcagggggag gaggagcggc cgcgccgccg catgtggcgg acgcggctcc
    15181 gggcgatcga ccggcacgcc gggcgcggca gcctgctcga catcggctgc ggggacgggc
    15241 tcttcctcga ggaggcgcgc cgcgccggct ggcgtgggga cgggatcgag ttctcccccg
    15301 aaggggcgcg ccgcgccgcg gcccggctcg ggcggccggt ggcggtgggc gatctcgcga
    15361 agtcgaaggt cctgccgggt ccgtacgacg tcgcgaccct gtggcacgtc gtcgagcatc
    15421 tgccggagcc gcggccgatg atcgaagcgg cgcgccgccg gctgcggcgc ggcgggctgc
    15481 tcgtcgtcgc ggtgccgaac accgacaacc tgccgatgcg cgccgcctac cgcctgggac
    15541 gcggccgccg gctgccgctg tgggagccgg gggcgcgcga gccgcacgtc agccatttca
    15601 caccggcgac gctgcgcgcg cttctgctgc gcggcggctt cgacccgctc gtgatcgagc
    15661 ccgatcgttg cgctctgacc ccggccaagc ggctgatcga cgccgtggcg gcatctcttt
    15721 ccattctcgg cggcgtcctc gtcaccgacg ccatcgccgt cttcgcacgg aggcccgcat
    15781 gaatccgccc gtaccgttcc cgctcaaacc ggtcaccgtc ccccccgatc ggcacacggc
    15841 cgagcggctg cgggccgccg cgcgccaggt cgtgctcgcg gcgcggacgc ccgtcctcct
    15901 ggcggcgcgg ttggtgcgac gccgtgtcac ccccccggcg gtcacgctgg tgcaccggct
    15961 cctggtgatc cggacggatc ggatcggcga catggtcctc agcaccgccg ccctggccga
    16021 tctcaaggcg cacttccgcc acgcccgtat caccgtgctc gcacccgagg gaccgctcca
    16081 ggtgctgcgc gggcacccgg cggtcgacgc gctcgtgccg ctcaaggatc gccggcttcc
    16141 ccccgacctc atggggaaat tcgatctcgc gatcgacctc acccccgatg cgcggctgct
    16201 cggggcccgc ctcgcggcgg ccacgggcgc tccgtggcgc accgggctct ccgcgtatgg
    16261 ccggcaggcc ttcttcaccc tgccgacagc caaggctcgg ccggacctcc acatcgtcga
    16321 tctcaaccgg gatctcctgc gggcgatcgg tgtcccctcc cccgccgcgg agccgaccct
    16381 ccacctccag ccgtcggagc gggcggcggc ccaggcgcgg gtctccgccc tgggagcggc
    16441 cgctccccgc gtcgtcgtcc accccggggc gcactacccg tcgcagcgct gggcggccgg
    16501 tcgcttcgcc gaggtgatcg cccggctcac cgagacggtc ggcgccgcct gcatcgtcgt
    16561 cgcgggcccc ggcgaggagg acctcgcgga ggcgatcggc gccgccactc ccgacgcgct
    16621 cctgcccgga ccactcgacg tccggggcct gatggggatg atcgcggtcg ccgacctctt
    16681 cctcggcaac aactccggcc cactgcacat cgccgcggcg ctcggcgtcc ccaccctctc
    16741 cgtgatgggg ccgaccgacc cgcgccgctt cgcgccgcgc cgacccgagg acacggtgct
    16801 gcgcctcgag ctcccctgct cgccgtgcca gcgggcccgc tgctggcacc acacctgcct
    16861 cgaggcgatc ccggcggagg tggtcgccgc ccgggcgcgg gacatcctcc gggccgagcc
    16921 cgcggagcag gcggcgtgag ccggagtctc agcgtgctcc acctcatcac ccgcctggag
    16981 cggggcgggg cctccgactg cacgctgtgg caggcgatcg gcgcggcgcg ccgcgggcac
    17041 cgggtgacgc tcgcgtgcgg gccgtcggag atcgagacac ccctgatcgc gcgggcgcac
    17101 cggcaggccg gcctcgagat cctgacgctg ccggcgctcg gccggacgct ccgcccggga
    17161 cgcgatctgc gcgccctcgc ggcgatcgcg acgctcatca agagccggcc cttcgacgtc
    17221 atccacctgc acacctccaa ggcgggggcg ctcgggcgca tcgccgcggg gcttctcggc
    17281 cagagccagc gggtcgtgca ccagccgcac gggcacctct tctacggcta ctacggacgc
    17341 atgggcggga ggatggtgct cctcgcggag cggctcctcg cgccgctggc gaggcggcac
    17401 gtcacgctca ccgggcgggg agcgcgcgag cacctggcgc gcggcgtcgg ccaggccgac
    17461 cagttccacg tcatcccgtc gggggtggat ttcaggccgc tgcgcaaggc cgcccgccaa
    17521 cacggtgcgg cgcggcagcg cctcggatac gccgcctcgg agttcgtggt gatgtcgctc
    17581 gcccgcctcg agccggtgaa gggggccgag gagacactgg aggcgttcgc cgcggcggcg
    17641 cggagccgcc cggatctgcg tctccacctc ggcggcgacg gccccctgct cgagtcgctc
    17701 cgcgcgcggg cgcgggccct cgggatcgcg gaccgcgtcc gcatcgacgg cgtctgggcc
    17761 gcgcccgagg atctcctgcc ggcggccgac ctgttcgtcc tcgcgccgcg caacgaaggg
    17821 atggggcggg ccgtcatcga ggcgctggcg ctgtccctgc cggtgatcgc gaccgccgtc
    17881 ggcggcctcc ccgaggtgct ggaggacggc gccgcgggga tcctggttcc gccccgcgac
    17941 acggcggcgc tttcggaggc gatcggccgc atcgcggacg atcccgccct cgcccgtgcg
    18001 ttcggacgcc gcggtcgcgc ccgcgccgtg gagttcggcg cgggccgcat ggtgcaccgc
    18061 gtgctcaagc tctaccggga catcgccgca tgaacgttgc ccgtgaggaa cgcggtccct
    18121 atgttgcgat ccgcgaaggt ttgagcggga ggagagcggc gtgatcctgg cggcgcacca
    18181 gcctcagtac ctgccctggc tcggctattt cgacaagatg gcgcgcgccg accgcttcgt
    18241 cctgatcgac acggtgcagt acaagaagaa cgaatggcag aatcgtaatc ggatccgcgc
    18301 ggcctccggc tggcagtggc tcaccgtccc ggtgcacgcc cgcttccccg cccgcatcga
    18361 cgaggtggcc gccgatgagg ccgccccgtg gcgccgcaag cacgccgcga ccctgcggca
    18421 ggcctacgcc cgggcgccgc accgggacgc cgtccttccg tcgctcgagc gcctgctgga
    18481 acgccccctc catcgtctcg ccgagatcaa catcgagtcg atccgcctgc tcgccgatct
    18541 gctggaggtg cgcaccgaga cgatcctcgc ctcggacatc gaaggcatcc cggatgggcc
    18601 ggacgcgcgc ctcatcgccc tgtgccggag gttcggggcc gatcgttatc tctccggagc
    18661 cggcggacgc gactacatgg acctcgacgc ctggaagcgg gccgggatcg aggtcgtgtt
    18721 ccaggagttc cgcggaccgg tctacgcgca gggacgctcc ggcttcgagc cgaacctgtc
    18781 ggcggtcgat ttcctcgtgc actgcggccc cgggccgatc gagcggccgg ccgcgccggc
    18841 acgggtggcg gcgtgaacat cctggcgatc ggcgcccacc cggacgacat cgagttcggc
    18901 tgcggcgg
//
LOCUS       DASRIQ010000176         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_68820, whole genome shotgun sequence.
ACCESSION   DASRIQ010000176 DASRIQ010000000
VERSION     DASRIQ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68820"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFD06_04420"
     CDS             complement(<1..520)
                     /locus_tag="VFD06_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN02811.1"
                     /translation="MGYLLVILSALAYSSLGIFGKIGLEADLPLASFLAARFALAATA
                     LWTVVLIVPRLRQALAAAARARAGLLLWGMVGLAGQSALYFAGLRTLSASLMEVLLYT
                     CPAFLALIEWGRTGVRPKRRVVAALLFALFGTWLAAAPAFGGSTLTGVILGLLTGLWY
                     ASFLVPLDRLTKG"
     gene            585..1760
                     /locus_tag="VFD06_04425"
     CDS             585..1760
                     /locus_tag="VFD06_04425"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HZN02812.1"
                     /translation="MSISAGVGLSQNADAVRAAREAAGAALERIGGERADWGVVFATP
                     AHRPQYAPLLAEAQKTLGTASLAGCSGAGVIGAGEEIEGGHGVSVLAVRSETLRAETH
                     LLPGGDDFGRQAAIDLASRLPEEGTLVAFPDPFAIRPQLMLAELRAAGSHVAVIGGAA
                     GGAPRHPATFQFFGRNVATRSVAALHLRGRGARGTIGITQGCQPLGPPCRVTRASENI
                     VLMLDGKPALDVLRGRLPAGVSDALERLGGHLFVGFAPDPDQEDIRPGEYLVRPIVAI
                     DPSRGALLMAEDVREGQPLAILLREGQAARDDLKTMLEGLAGRGGPYKFGLYFNCAGR
                     GSALYGIPGVDAAYIAQRFGDLPIAGFFGNAEMAPLQDRNVLFTHTGVLALFGEGEE"
     gene            1757..>2212
                     /locus_tag="VFD06_04430"
     CDS             1757..>2212
                     /locus_tag="VFD06_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02813.1"
                     /translation="MKDRIALRDDLLVFLRNARGAVASRDLAARFLAIPRADEETCRR
                     LLTPILSGVRGLEHQAGAGWVFTGAGHAIAEVAGPSAGADDPPGEAAVASVETPPLPT
                     ERRNEDNDSETLARLDFVALATDGIGPGGSGAPRAVAIVPVLGGEALHEE"
BASE COUNT          313 a          799 c          833 g          267 t
ORIGIN      
        1 tccccttggt gagccggtcg agcggcacga ggaaggaggc gtaccacagc cccgtcagga
       61 gcccgagaat cacccccgtg agcgtgctcc cgccgaaggc cggagcggcg gcgagccagg
      121 tcccgaagag cgcgaacagg agcgccgcga cgacgcgccg cttggggcgc acgccggtcc
      181 tcccccactc gatcagggcg aggaaggcgg ggcaggtgta gaggagcact tccatgaggc
      241 tggcggagag agtccgcagc ccggcgaagt agagggccga ctgtccggcg aggccaacca
      301 tcccccagag gaggagcccg gcgcgcgcgc gcgcggccgc ggcgagcgcc tggcgcagcc
      361 gcggcacgat caggacgacg gtccacaggg ccgtcgccgc cagcgcgaag cgggcggcga
      421 ggaacgacgc gagcggcagg tcggcctcga ggccgatctt cccgaagatg ccgagcgacg
      481 agtaggcgag cgcggagagg atgacgagca ggtagcccac ggggcggtga ttataggggc
      541 ccgtcacgcc gggaagcgcg cggcccccgt ggtatcgtcc cggcgtgagc atctccgcgg
      601 gagtcggcct ctcccagaac gccgacgcag tgcgggcggc gcgcgaggcg gcgggagcgg
      661 cgctcgagcg gatcggcggc gagcgcgccg actggggggt cgtcttcgcc accccggcgc
      721 accggccgca gtacgcccct ctcctcgccg aggcgcagaa gacactcggc acggcgagcc
      781 tcgccggctg cagcggcgcc ggcgtgatcg gcgccggcga ggagatcgag ggggggcacg
      841 gcgtgagcgt tctggcggtg cgctccgaga cgctgcgcgc cgagacccac ctcctgcctg
      901 gcggcgacga cttcgggcgc caggcggcca tcgatctcgc ctcgcgcctg cccgaagaag
      961 gcaccctggt ggcgtttccc gatccgttcg ccatccggcc gcagctgatg ctcgcggagc
     1021 tgcgcgcggc cggcagccac gtggcggtga tcggcggcgc cgccggcggg gcgccccgcc
     1081 accccgccac cttccagttc ttcggacgca acgtcgccac ccggtcggtc gccgccctgc
     1141 acctgcgcgg acgcggggcg cggggaacca tcggcatcac ccagggctgc cagccgctcg
     1201 gccccccgtg ccgggtcacc cgcgccagcg agaacatcgt cctcatgctc gacggaaagc
     1261 cggcgctcga cgtgctgcgc ggccggcttc cggccggcgt gtcggacgcg ctcgagcggc
     1321 tcggcgggca cctgttcgtc ggattcgcgc cggacccgga tcaggaggac atccgcccgg
     1381 gggaatacct cgtccgcccg atcgtcgcca tcgacccgtc ccgcggcgcg ctgctcatgg
     1441 cggaggacgt ccgggagggg cagccgctgg cgatcctcct gcgcgaagga caggcggcgc
     1501 gggacgatct caagacgatg ctggaggggc tcgccgggcg cggcggtccc tacaagttcg
     1561 gcctctactt caactgcgcc ggacgcgggt cggccctgta cggcatcccc ggcgtggacg
     1621 ccgcttacat cgcccagcgc ttcggcgacc tgccgatcgc cggcttcttc ggcaacgccg
     1681 agatggcgcc gcttcaggat cgcaacgtcc tgttcacgca caccggggtg ctggcgctgt
     1741 tcggcgaggg ggaagaatga aggatcgcat cgcgctgcgc gacgatcttc tcgtgttcct
     1801 caggaacgcc cgaggagcgg tggcgagccg cgacctcgcc gcgcgcttcc tcgccatccc
     1861 ccgcgccgac gaagagacgt gccggcggct cctgacaccg atcctctcgg gcgtccgcgg
     1921 tctggagcac caggccggcg cggggtgggt cttcaccgga gcgggccatg caatcgcaga
     1981 ggtcgccggc ccgtccgccg gcgcggatga tccgcccggg gaggcggccg tcgcctccgt
     2041 ggagacgccc cctctcccga cggagcgacg caacgaggac aacgattcgg agacgctcgc
     2101 gcggctcgat ttcgtcgcgc tcgccaccga tgggatcggc cccggtggat cgggggcgcc
     2161 gcgcgccgtg gcgatcgttc cggtgctcgg cggcgaggcg ctccacgagg ag
//
LOCUS       DASRIQ010000177        13122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1638915, whole genome shotgun sequence.
ACCESSION   DASRIQ010000177 DASRIQ010000000
VERSION     DASRIQ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638915"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFD06_04435"
     CDS             complement(<1..362)
                     /locus_tag="VFD06_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZN02814.1"
                     /translation="MTRGRAAALIVPALVAAGILHAPSGATARPALPLAATAPAGTTL
                     SPTASLESRVREIRLDNGMRFLLLRRGTAPVFAGILRFRVGSADDPGGSTGLAHLFEH
                     MAFKGTSTIGVTDAAKERP"
     gene            complement(359..1216)
                     /locus_tag="VFD06_04440"
     CDS             complement(359..1216)
                     /locus_tag="VFD06_04440"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN02815.1"
                     /translation="MRPRVPLSPATAAVLAAGIALASSAGGAAENPAPPATPPPPAAQ
                     AKPQVRNIGGPGDYPLREKLVNRMIRDPELASARPSIILVNGGAVFSGEVPTWTVRRR
                     ALALAGSVRGIVNVTDQMTVPRGNVKDEEILKGMAAILKDWKEPLEIADLDISVQDGV
                     ATLRGKVKNFAARVRAEEAAGTVLGAQTIVNRLRPANAPAGTDDPSIRKAIAKYLGDF
                     RDYPYPATLEVAVKDGRATLTGEVPIYLGRQQAGTMAALVGGVQAVENRIRVEPSSQL
                     PDTTVREVP"
     gene            complement(1254..2210)
                     /locus_tag="VFD06_04445"
     CDS             complement(1254..2210)
                     /locus_tag="VFD06_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02816.1"
                     /translation="MITGCATSATDATRSRGAMAAAVAATLLLLATGRAGTSGAFAAE
                     APAAVDPDTPGDLRDRFEKIEKLLRKAVDALEDKDQARVSFLLQRADEQMARFEAGSN
                     VARYLASLDAARQAADQGTLPAAETALRAARTALPPLGDYTVARSMEIAFRAALQGAA
                     DGSVEEFRKGLLEMDEATLAGPIAARCREVREAMGRTRKAMGRNDAVAGRKELDAAVA
                     ALARIDYGGLLSRARSGLQLASELLRGRATLAARDQTQRGLKELAEAIPIAPEADREP
                     LTRAQEEARTVWRRLNRAEKDDPDRLAAASEQIETLRRAQRS"
     gene            complement(2179..2538)
                     /locus_tag="VFD06_04450"
     CDS             complement(2179..2538)
                     /locus_tag="VFD06_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018745561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZN02817.1"
                     /translation="MDREHFEQLVGEAIDSLPDDFRSRMNNVAVVVEDLPSPRDERRF
                     ARGRLLLGLYQGQPLTKRDSRYGMAFPDKITIYQANIEALCRTDDEIRRQVRKTVLHE
                     IAHHFGIDDHRLRDLGY"
     gene            complement(2632..3081)
                     /locus_tag="VFD06_04455"
     CDS             complement(2632..3081)
                     /locus_tag="VFD06_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZN02818.1"
                     /translation="MNWLVKEEPDSYSFDRLVEDEGTDWSGVRNPLAQRHLRAIRRGD
                     GIFYYHTGSERAVVGIARAASDAGPDPADRTGRNATVRIVPVKRLAHPVPLAALKADP
                     AFAHHPIVRMGRLSVMPISDREWSLILKRAERGDAGNEVPRKPRRKK"
     gene            3123..3839
                     /locus_tag="VFD06_04460"
     CDS             3123..3839
                     /locus_tag="VFD06_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZN02819.1"
                     /translation="MRRDSRRRRGPGQGTRRRERRGAILFDFNGVLMEDEGLHFRCYL
                     TILAPLGIAFDRRRYEARYLPYDDRAAVARMLSDAGRAAPPALVRRLVATKRRLFRAV
                     VRREGVAIEPAVRRLVRRLARTRTLVIVSGAARSEILAALAAARLRTAFRFIVAAESV
                     RRGKPHPEGYRRALARLPLAARNGEGTMAIEDSPGGIKAARGAGLPVLGVATSYRPAE
                     LRRAGAFAVVPSLAFPGRFF"
     gene            complement(3836..5521)
                     /locus_tag="VFD06_04465"
     CDS             complement(3836..5521)
                     /locus_tag="VFD06_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN02820.1"
                     /translation="MRTVAAAVGLAAAALAGASCRVVRPGESVDLILHHGVVLTLDPA
                     RPEAQAVAIRGDRIVAVGADADILALAGDATRSVDLKGAFVLPGLIDAHGHVASLGER
                     LSSVDVRGVTSIEAIAAAVRARASEGLPEGAWITGGGWDQNLWPGEQFPDHRPLDAAS
                     PDRPVLLRRVDGHASWANLKAMQAAGISRDTPDPDGGAIVRDARGEPTGVFVDNAMAL
                     LEAAQPKASRAQTKDWIRLALKRCAAAGLTEIHDAGVSMEQAAAYRELADAGDLPLRV
                     YLMWDGTTPEPIDAMIATEPYVNHRDRLTLRAVKLMVDGAMGSRGAVFFDDYEDARGT
                     RGLFVTSPEEIESRAEKAMARGYQVCTHAIGDRGIREVIDAYAKALQAVPPGDRRPRV
                     EHVQCVRKDDLADFKRLGVIASMQPSHATSDMPWAEERVGMDRGRGLYAWRWVLEAGV
                     PIAAGSDFPVDPERPLVGLHSAVTRQAPDDSPAGGWHPDQAMTLLEAITAYTTTAAWA
                     AFEEKDKGRIAPGMWADLTILGEDLRAVPPARIDDVPVRATLVGGRFAYESSN"
     gene            complement(5559..6413)
                     /locus_tag="VFD06_04470"
     CDS             complement(5559..6413)
                     /locus_tag="VFD06_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN02821.1"
                     /translation="MAAALAATLLWGMNFSAAKRVLEEVDPLAVAAARALGGVVVFAA
                     LLTILDGPRGLAWPRLRRAVPLGLLGVFANQIFFIEGLKRTSAAHSAVLISLLPLYVL
                     LLSVAFGQERLNLWKGAGIATAFAGVVLVASEHGGRSAGAATLQGDLITMCGGLSFAA
                     YTVLGRPVVRDLGPLRSTALAFAGGGTAILLVAAPAARRQDWRALSPAALSGLLYVVV
                     GATVLAYLFYYLALDRLEPSKVAVFMYLQPVVAALVAYFVTGEALTPRFFAGGTLILA
                     GVLVAERG"
     gene            complement(6470..8389)
                     /locus_tag="VFD06_04475"
     CDS             complement(6470..8389)
                     /locus_tag="VFD06_04475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZN02822.1"
                     /translation="MKRSQLCPALVTGLALFCGLALLLGGLAACGSGGPAVGPQEGAA
                     HPMVGKPAPDFVGEPPVGGWLPLRNLKDKPIAILFYRPGAPFTRELAAAFKGFRSDPT
                     MAPTVFLGIAADDMENIKRFMAETGDALPTLRDPGSITRSYGVGDAPTVILLDSRRFV
                     RFRLDGFAGRHFRARVEAVAEAVRRLPNAEAPQVDALPIEYTGHPRAPVFSGRDLDGR
                     VFDTAAWKGQVLVLIFFDQECPHCQSDLPRLGPVLREYRGRGVRALGVSARDLRGGMR
                     GFLKQHDADFPVLIDTDRAVFGRFQSKRTPDLFIIDKEGLIRFRERGDRPDRAELLRL
                     QLRVVLGEEAPQAIAASLPKGRYVGDAACAACHEREMQDWLLTPHSIAWDSLSQGDKW
                     KDPECVKCHVTGMGDPGGFKSPEETLHLVNVQCEVCHGMAGGHATGGGMDVDALSNVC
                     VTCHTGKFVLTFTLDEALPLVAHQDHPDLDRLFRYSDAQRQRLEQINKRRMEKFKSGV
                     AHVGSDACRPCHQKEYEQWSHTPHAAAFATLLKQGRNTDPACQSCHTTGFGLRRGFGD
                     ALATASLTGVQCEVCHGPGEDHVNAPPALKKETIYGITDQCSFCIIQGVCATCHDAKN
                     DPDFDIEAALPKVKH"
     gene            8512..8817
                     /locus_tag="VFD06_04480"
     CDS             8512..8817
                     /locus_tag="VFD06_04480"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN02823.1"
                     /translation="MAEWFKVAGGGTLADGQARVVEVNGRSIALFKTGGRMYATDSAC
                     AHRGGPLAEGTIEADSIVCPWHGFRFDLKEGACSTNPALRLACYPARLAGDDVEVEM"
     gene            8886..9464
                     /locus_tag="VFD06_04485"
     CDS             8886..9464
                     /locus_tag="VFD06_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02824.1"
                     /translation="MRPRPGRFVPWTALALLACVLGASPARAEILYNPMGWKFPNIIT
                     AAKEAIHVSDRTPTIPGKETLNKGYRRPNGTRCMTYEIEGKIFGVEIDEDGKPPFEYG
                     IMDADGDGKFETKIVYHKAQKDHFYVPAWVIDHYFSVHPDVERGPGGVPVMPSLNAAA
                     AAPAPNRKKASAPAPPPKKKPGVPSPGDLATP"
     gene            complement(9563..11296)
                     /locus_tag="VFD06_04490"
     CDS             complement(9563..11296)
                     /locus_tag="VFD06_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZN02825.1"
                     /translation="MIERRRRWRRRVAPGLATLAGVLAAARLLSGEALPETPPAAAAA
                     TAPVLFVDIAKAAGVTLLNRSGSREKDTINETVGNGVCLADFDGDGFLDIFLPNGQPR
                     GGAKASSALYRARGDGTFEDRTEGSGTALPGYWAQGCAAADYDADGRTDLFVTGFGRY
                     WLLRQTDRLRFGDVTAAAGLAGGRGWSTGAAFADYDRDGWIDLYVSHYVDYDQNSPPL
                     PPTGSGPNCNYRGYPVMCGPRGLKPQAGQLFRNLGGRFRDVTAAAGVNAAYPGYGLGA
                     VWSDLDDDGDLDLFVASDSTPNYLFRNDGGRFKDVATTSGVAYAEDGRTQASMGVDAG
                     DYDNDGRFDLVTTNFSHDYTTVYHNEGGLLFLDVALESGVGPATMPSLGWGVHFLDYD
                     GDGRRDLFEANGHVYPGIDALNLGTAWAQANQLFRNEGGVRFREVTATAGPAFQEKHS
                     ARGSAVGDLDNDGDPDLVVNNMDGPPSVLRNDAPLHRWIGLRLEGAGKNRAAIGAKVT
                     LTNGPLRQVDEVRAGGSHNSSSDPRLLFGLGTFSGPVRIEIRWPTGNKQTVTGLTPDR
                     YHTLKEPPPTR"
     gene            complement(11289..12410)
                     /locus_tag="VFD06_04495"
     CDS             complement(11289..12410)
                     /locus_tag="VFD06_04495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN02826.1"
                     /translation="MSRAAIVALGAAAAIACSETPEHALAEARRLQDAGRSEAAMKKV
                     DTLLEKNPGLFEARLYRAVLVGAQTGREEEGLSALRQLAQAEPRRTGVHRAMGQILAR
                     TGRFAQAVNQFEKELAIDPADTETLTDLGLFYFQTGQTDQAEDRLRRATADGAGNARA
                     YRALAEISLRAGRIEEGLAQQRRALELTPDDADLVVTHARALGAYGHAPDAKALLDAA
                     VARGLKDAALPAEQARQAREGLDYVGAVAAYRRSLDIDPTRSATLMELGKTLLLMGRR
                     DEARAAFEAGRDASPSDPYPWFYLGTMSADDGALEPAISDLRRSLELDPVNPKAHYAL
                     AQALLRSGRPDEAQAEFARHAESLDFLRSNRTQGTSTLD"
     gene            complement(12511..12711)
                     /locus_tag="VFD06_04500"
     CDS             complement(12511..12711)
                     /locus_tag="VFD06_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZN02827.1"
                     /translation="MPTGKVKWFNNSKGYGFIQQEDGTDIFVHFSAIAGEGYKTLDEG
                     QSVQFEVTQGPKGLQAANVSKV"
BASE COUNT         1854 a         4568 c         4770 g         1930 t
ORIGIN      
        1 ggccgctcct tcgcggcgtc ggtgacgccg atggtcgagg tccccttgaa cgccatgtgc
       61 tcgaagaggt gcgccagccc ggtcgaaccc ccggggtcgt ccgccgagcc gacgcggaag
      121 cgcaggatcc cggcgaacac cggcgccgtt ccgcgcctca gaagcaggaa gcgcatcccg
      181 ttgtcgaggc ggatctcccg aacccggctc tcgagcgagg ccgtggggga gagcgtcgtc
      241 cccgcgggcg cggtggcagc gagcgggagc gccgggcgtg cggtggctcc ggacggggca
      301 tggaggattc cagcggcgac gagcgccgga acgatgaggg cggccgcccg cccccgggtc
      361 acgggacttc ccggacggtc gtgtccggca gctgcgacga gggctcgacg cggatacggt
      421 tctcgaccgc ctgcaccccc ccgacgagcg ccgccatggt gccggcctgc tggcggccga
      481 ggtagatggg gacctcgccg gtcagggtgg cgcggccgtc cttcaccgcc acctccagcg
      541 tcgcgggata gggatagtcg cggaagtcac cgaggtattt ggcgatcgcc ttgcggatgg
      601 agggatcgtc ggtgccggcc ggggcgttgg cgggccgcag gcggttgacg atcgtctgcg
      661 ccccgaggac cgtcccggcc gcctcctcgg cgcgcacgcg cgcggcgaag ttcttcacct
      721 tcccccgcag ggtggcgacg ccgtcctgca cgctgatgtc gaggtcggcg atctcgagcg
      781 gctccttcca gtccttcagg atcgccgcca tccccttgag aatctcctcg tccttgacgt
      841 tgccgcgcgg aacggtcatc tggtcggtca cgttgacgat gccgcgcacc gatccggcga
      901 gcgccagcgc ccggcgccgc accgtccagg tcggcacctc tccggagaag accgcgccgc
      961 cattgaccag gatgatcgaa ggccgcgcgc tcgccagctc cgggtcgcgg atcatccgat
     1021 tgaccagctt ctcccgcagc gggtagtcgc cggggccgcc gatgttgcgc acttgaggtt
     1081 tcgcctgggc ggcggggggg ggcggcgtcg ccggaggtgc ggggttctcg gcggcccccc
     1141 cggcggacga cgccagggcg atgccggcgg ccagcaccgc ggcggtggcg ggactcagcg
     1201 ggacacgggg tctcatgacg gggcggtatt ctgacacaag ggttcgacca ggtctaagaa
     1261 cgctgcgccc gccgcaaagt ttcaatctgc tcgctcgccg ccgccagccg gtccggatcg
     1321 tccttttcgg cgcggttcag gcgccgccag acggtgcgcg cctcctcctg ggcccgggtg
     1381 agcggttccc ggtccgcctc cggggcgatg gggatcgctt cggccagctc cttgagcccc
     1441 cgctgcgtct ggtcgcgcgc cgcgagagtg gcccggccac gcagcagctc cgaggcgagc
     1501 tgcaggccgg accgggcgcg tgagaggagc ccgccgtagt cgatgcgggc gagcgctgcc
     1561 acggcggcgt ccagctcctt ccggccggcg acggcgtcgt tccggcccat cgccttgcgc
     1621 gtccgcccca tcgcctcgcg cacctccctg cagcgagcgg cgatcgggcc ggcgagcgtc
     1681 gcctcgtcca tctcgagaag gcccttgcgg aactcctcca ccgagccgtc cgccgccccc
     1741 tggagcgccg cccggaaggc gatctccatc gagcgggcga cggtgtagtc cccgagcggc
     1801 ggcagggcgg tgcgggcggc acgaagggcc gtctccgccg ccggcagcgt cccctgatcg
     1861 gccgcctgcc gcgccgcgtc cagggaagcg aggtagcgcg ccacgttcga gccggcctcg
     1921 aaccgggcca tctgttcgtc ggcgcgctgc agcaggaacg acacgcgcgc ctggtccttg
     1981 tcctcgagcg cgtccaccgc cttgcgcagg agcttttcga ttttttcgaa gcggtcccgc
     2041 aaatcgcccg gtgtgtccgg gtcgaccgcg gccggggcct cggcggcgaa ggcgccggaa
     2101 gttcctgccc gacccgtggc caggaggagc agcgtcgccg ccacggcggc ggccatcgcc
     2161 ccccggctgc gcgtcgcgtc agtagccgag gtcgcgcagc cggtgatcat cgatgccgaa
     2221 gtggtgggcg atttcgtgca ggacggtctt gcgcacctgg cgccggatct cgtcgtcggt
     2281 tctgcagagg gcctcgatgt tggcctggta gatggtgatc ttgtccggga acgccatgcc
     2341 gtagcgggag tcgcgcttcg tcaggggctg cccctggtac agcccgagca agagccgtcc
     2401 gcgcgcgaag cggcgctcgt cgcgcggaga ggggaggtcc tcgaccacga cggcgacgtt
     2461 gttcatgcgg ctccggaagt cgtcagggag ggagtcgatc gcctcgccca ccagctgctc
     2521 gaagtgctcc ctgtccatgg cggcattcta tccaaggtgg gccggtgatt gcggcaggcg
     2581 aggcggagac cgcgccgcgc cggcgccgca gccgcgggtg ggacggccga tctacttttt
     2641 ccggcggggc ttgcgtggca cttcgttccc ggcatcgccc cgctcggcgc gtttgaggat
     2701 caggctccat tcccggtcgc tgatcggcat gaccgagagc cgccccatcc gcacgatcgg
     2761 gtgatgcgcg aacgccggat ccgccttcag cgcggcgagc gggaccgggt gcgccagacg
     2821 cttcaccggg acgatccgga cggtcgcgtt gcgtccggtc cggtcggccg ggtccggccc
     2881 ggcgtcggag gcggcgcggg cgatgccgac gacggcccgc tcggagccgg tgtggtagta
     2941 gaagatgccg tccccgcggc ggatggcgcg cagatgccgc tgcgccaggg gattgcgcac
     3001 gccgctccag tcggtcccct cgtcctcgac caggcggtcg aacgaatacg agtcgggctc
     3061 ctccttgacg agccagttca tgaaggcgat cctcccgcga tccttcgacg atgaggtggt
     3121 ggatgcggcg cgattctagg aggcggagag gaccagggca agggacccgc cggcgtgaac
     3181 gtcgcggggc gatcctcttc gacttcaacg gcgtcctcat ggaggacgag ggactgcact
     3241 tccggtgcta cctcacgatc ctcgccccgc tcggcatcgc gttcgaccgg cgccgctacg
     3301 aagcgcgcta cctgccgtac gacgaccggg ccgcggtggc gcggatgctg tcggacgccg
     3361 gacgtgcggc gccgccggcg ctcgtgcggc ggctggtggc gacgaagcgg cgcctgttcc
     3421 gtgcggtggt gcggcgggaa ggggtggcga tcgagccggc ggtgcggcgg ctggtgcggc
     3481 gcctggcgcg gacgaggacg ctcgtcatcg tctccggggc ggcgcgctcg gagatcctcg
     3541 cggcgctcgc ggcggcgcgc ctgaggacgg cgttccgctt catcgtcgcg gcggagagcg
     3601 tccgccgcgg caaaccgcac ccggaagggt accggcgcgc gctcgcgcgg ctccctctcg
     3661 cggcccggaa cggggagggg acgatggcga tcgaggattc cccgggcggc atcaaggcgg
     3721 cgcgcggtgc agggcttccg gtgctcgggg tcgcgacgtc gtatcggccg gcggaactcc
     3781 ggcgcgccgg ggcgttcgcc gtcgtgccga gcctggcctt ccccggccgc ttcttctagt
     3841 tcgaggactc gtaggcgaag cggccgccga ccagggtcgc ccgcaccggg acgtcgtcga
     3901 tgcgggccgg cgggacggcg cgcaggtcct cgccgaggat cgtgagatcg gcccacatcc
     3961 ccggggcgat gcgccccttg tccttctcct cgaacgccgc ccacgcggcg gtcgtcgtgt
     4021 aggcggtgat cgcctccagg agcgtcatcg cctggtcggg gtgccagccc ccggcggggg
     4081 agtcatcggg agcctggcgt gtgaccgcgc tgtgcaggcc gacgaggggg cgctccgggt
     4141 ctaccggaaa atcggagccg gcggcgatcg ggacgccggc ctcgagcacc cagcgccagg
     4201 catacagccc gcgcccccga tccatgccga cccgctcctc ggcccacggc atgtcgctcg
     4261 tggcgtggct cggctgcatc gaggcgatca ccccgagccg cttgaagtcg gcgagatcgt
     4321 ccttccggac gcactgcacg tgctcgacgc gcggccgccg atcccccggt ggcacggcct
     4381 gaagcgcctt ggcgtacgcg tcgatcacct cgcggatgcc gcggtcgccg atggcgtggg
     4441 tgcagacctg atagccgcgc gccatcgcct tttcggcgcg gctctcgatc tcctcggggg
     4501 aggtgacgaa gaggccgcgc gtcccgcggg cgtcttcgta gtcgtcgaag aagaccgcgc
     4561 cgcgcgatcc catggcgccg tccaccatca gcttcacggc gcgcagcgtc aggcggtcgc
     4621 ggtggttgac gtacggctcc gtggcgatca tcgcgtcgat cggttcgggg gtggtgccgt
     4681 cccacatcag gtagacgcgc agcggcaggt cgccggcgtc ggccagctcg cggtaggcgg
     4741 ccgcctgctc catcgagacg ccggcatcgt ggatttcggt gaggccggcg gcggcgcagc
     4801 gcttgagcgc caggcggatc cagtccttgg tctgggcgcg cgacgccttc ggctgcgccg
     4861 cctcgaggag cgccatggcg ttgtccacga acacgccggt cggctctccc cgcgcgtccc
     4921 gcacgatcgc gccgccgtcg gggtcgggcg tgtcgcggct gatgccggcc gcctgcatcg
     4981 ccttgaggtt cgcccacgaa gcgtgcccgt ccacgcgccg gaggagcacc ggccggtcgg
     5041 gggaggcggc gtcgagcggc cggtgatcgg ggaactgctc gcccggccag aggttctggt
     5101 cccagccgcc gccggtgatc caggcgccct cggggagccc ctcggaggcg cgcgcccgca
     5161 cggcggcggc gatcgcctcg atgctggtga cgccgcgcac gtccaccgac gacagccgct
     5221 ctccgaggct cgcgacgtgc ccgtgcgcgt cgatgaggcc ggggaggacg aaggcccctt
     5281 tcaagtcgac ggagcgggtc gcgtctcccg cgagcgccag gatgtcggcg tcggcgccga
     5341 ccgcgacgat gcggtcgccg cggatcgcca ccgcctgagc ctccgggcgc gccggatcga
     5401 gggtcagcac gacgccgtgg tgcaggatca ggtccacgct ctctcccgga cggacgacgc
     5461 ggcaggaggc gccggccagg gcagccgccg cgagcccgac ggccgcggca accgttcgca
     5521 ccccctccgg acgcctccgc atcgccgcgg aggatacctc agccgcgctc ggcgacgagc
     5581 acgccggcga ggatgagggt cccaccggcg aagaaacggg gagtgagcgc ctccccggtc
     5641 acgaagtacg ccaccagcgc cgcgaccacc ggctgcaggt acatgaagac cgcgaccttc
     5701 gacggctcca gccggtcgag cgcgaggtaa tagaagaggt acgcgaggac ggtcgcgccg
     5761 acgacgacgt agagcaggcc ggagagggcg gccgggctca gggcgcgcca gtcctggcgg
     5821 cgcgccgcgg gggcggcgac gaggaggatg gcggtgcccc ccccggcgaa cgcgagggcg
     5881 gtggagcgca gcggcccgag gtcgcgcacg accggtcgcc cgagcacggt gtaggcggcg
     5941 aacgacagcc cgccgcacat ggtgatgaga tccccttgaa gcgtcgccgc cccggcggag
     6001 cgccccccgt gctccgacgc gaccagcacg acgccggcga aggcggtggc gatgccggca
     6061 cctttccaga gattgaggcg ctcctggccg aaggcgaccg aaagcagcag cacgtagagc
     6121 ggcagcaggg agatgagcac ggcggagtgg gccgcgctcg tccgcttcag cccctcgatg
     6181 aagaagatct ggttggcgaa gacgccgagc aggccgagcg ggaccgcgcg ccgcaggcgc
     6241 ggccaggcga gcccgcgcgg tccgtcgagg atcgtcagga gcgcggcgaa gacgacgacc
     6301 ccgccgagcg cccgcgccgc ggccaccgcc agcggatcga cctcctcgag aacccgcttc
     6361 gcggcggaga agttcatccc ccagaggagc gtcgctgcga gcgctgcggc catcccgggg
     6421 aacgcggcgg cgtgagggcc ggcggcgggc ccggccgccg cccgatcgct cagtgcttca
     6481 ccttggggag cgccgcttcg atgtcgaagt cggggtcgtt cttggcgtca tggcaggtgg
     6541 cgcacacccc ctggatgatg cagaaggagc actggtcggt gatgccgtag atcgtctcct
     6601 tcttgagcgc cggcggggcg ttgacgtgat cctcgccagg cccgtggcag acctcgcact
     6661 gcacgccggt caggctggcc gtggcgagcg catcgccgaa gccgcgcctc agcccgaagc
     6721 cggtggtgtg gcaggactgg cacgcggggt cggtgttgcg cccttgcttc aggagcgtgg
     6781 cgaaggcggc ggcgtgcgga gtgtggctcc actgctcgta ctccttctgg tggcaggggc
     6841 ggcaggcatc cgacccgacg tgcgccaccc ccgacttgaa cttctccatg cgccgcttgt
     6901 tgatctgctc gaggcgctgc cgctgcgcat cgctgtaacg gaacaggcgg tcgagatccg
     6961 ggtgatcctg gtgcgccacc agaggcagcg cctcgtcgag cgtgaaggtg aggacgaact
     7021 tccccgtgtg acaggtgacg cagacgttcg acagcgcgtc cacgtccatt ccgccgccgg
     7081 tcgcgtgccc gccggccatg ccgtggcaga cctcgcactg cacattcacc aggtgcagcg
     7141 tctcctccgg actcttgaag ccgcccggat cccccatgcc ggtgacgtgg cacttgacgc
     7201 actcggggtc cttccacttg tccccctggg agaggctgtc ccacgcgatg ctgtgcggcg
     7261 tcaggagcca gtcctgcatc tcccgctcgt ggcaggcggc acatgcggcg tcgccgacat
     7321 agcgtccctt cggcagcgag gcggcgatcg cctgcggggc ttcctcgccg aggacgacgc
     7381 gcagctgcag gcgcaggagt tccgcgcgat ccggccgatc cccccgctcg cggaaacgga
     7441 tgagcccctc cttgtcgatg atgaacaggt ccggcgtgcg cttcgactgg aaccggccga
     7501 acacggcccg gtcggtgtcg atgaggaccg ggaaatccgc gtcgtgctgc ttcaggaacc
     7561 cgcgcattcc gcctctgagg tcccgcgcgg agacgccgag cgcccgcacg ccccgcccgc
     7621 gatactcgcg cagcaccggc ccgaggcgcg gcaggtcgct ctggcagtgg gggcattcct
     7681 ggtcgaagaa gatgaggacg aggacctgcc ccttccaggc ggcggtgtcg aacacccgcc
     7741 cgtccagatc gcgcccggag aagaccggcg cccgcgggtg gccggtgtac tcgatcggca
     7801 gcgcatcgac ctgaggggcc tcggcgttcg gcaggcgacg caccgcctcg gccacggcct
     7861 ccacccgcgc gcggaagtgc cggcccgcga acccgtcgag ccggaaacgc acgaagcgcc
     7921 gtgagtcgag gaggatgacg gtcggcgcgt cccccacgcc gtagctgcgg gtgatggagc
     7981 cgggatcgcg cagcgtcggg agggcgtcgc cggtctccgc catgaaccgc ttgatgttct
     8041 ccatgtcgtc ggcggcgatg ccgagaaaga ccgtcggcgc catcgtcgga tcggagcgga
     8101 accctttgaa ggcggccgcc agctcgcgcg tgaacggggc gccgggacgg tagaagagga
     8161 tggcgatcgg cttgtccttg aggttgcgga gggggagcca ccctccgacc ggcggctcgc
     8221 cgacgaagtc gggagccggc tttccgacca tcggatgggc ggccccctcc tggggaccga
     8281 ccgccggccc cccgctgccg caggcagcga gcccgccgag aagcagggca aggccgcaga
     8341 acagagcgag gcccgtgacg agtgcgggac acagctgcga acgcttcatc gaatcctgcc
     8401 agggacgaga ccgcacgccg gaaccgctgt cctgcgggct tctccgccgc gggcccggga
     8461 gtataggcta ggacccgccg atttcaccgt ggaagctgag gaggcctggt catggcggag
     8521 tggttcaagg tggcgggagg cgggaccctg gccgacggcc aggcgcgtgt cgtcgaagtc
     8581 aacgggcgct ccatcgccct cttcaagacc ggcggccgga tgtacgcgac cgacagcgcc
     8641 tgcgcccatc ggggcgggcc gctggccgag gggacgatcg aggcggacag catcgtctgc
     8701 ccgtggcacg gcttccgctt cgacctcaag gagggcgcct gcagcaccaa tccggccctc
     8761 aggctcgcct gctacccggc gcgcctcgcg ggggacgacg tcgaagtcga aatgtgacgg
     8821 ccgaaaccct ataatcggcg ctcctttcga cgaacgggga tctctgccac atgtccgaca
     8881 tcctcatgag accgcgaccg ggacgcttcg ttccctggac ggccctggcg cttctcgcgt
     8941 gcgtcctcgg cgcctcgccg gcgcgcgccg agatcctgta caacccgatg ggctggaagt
     9001 tccccaacat catcacggcc gccaaggaag cgatccacgt cagcgaccgg accccgacca
     9061 ttccgggcaa ggagacgctc aacaagggct accggcgtcc gaatggcacg cgctgcatga
     9121 cctacgagat cgaggggaag atcttcggcg tggagatcga cgaggacggc aagccgccgt
     9181 tcgagtacgg gatcatggac gccgacggcg acgggaaatt cgagaccaag atcgtctacc
     9241 acaaggcgca gaaggatcac ttctatgttc cggcctgggt gatcgatcac tacttcagcg
     9301 tccaccccga cgtggagcgc ggcccgggcg gcgtgccggt catgccgagc ctcaacgcgg
     9361 cggccgcggc tcccgccccc aaccggaaga aggcatccgc accggcgcct ccgccgaaga
     9421 agaagcctgg cgtgccttcg ccgggggatc tcgcgactcc ctgagctgaa tcagacttaa
     9481 tcagcccctc ggacccgcca accgcgactc gttccgccac aggggccaat tcgccgcgca
     9541 agaatcctcg cggcccttgc ggtcagcggg tggggggcgg ttctttcagg gtgtggtagc
     9601 ggtcgggggt gaggccggtt acggtctgtt tgttgccggt gggccagcgg atttcgattc
     9661 ggacggggcc ggagaaggtg ccgaggccga agaggaggcg tgggtcgctc gaggagttgt
     9721 gagagccgcc ggcacgcacc tcgtcaacct ggcgcagggg gccgttggtg agggtcacct
     9781 tcgccccgat cgccgcgcgg ttcttcccgg ccccttcgag gcgcaggccg atccagcggt
     9841 gcagtggcgc gtcgttgcgc agcacgctcg gcgggccgtc catgttgttg acgaccagat
     9901 cggggtcgcc gtcgttgtcg aggtctccga ccgccgatcc gcgcgcgctg tgcttctcct
     9961 ggaacgccgg gccggcggtc gcggtcacct cgcggaagcg cacccctcct tcgttgcgga
    10021 agagctggtt cgcctgcgcc cacgcggtgc cgaggttgag cgcgtcgatg ccgggataga
    10081 catggccgtt cgcctcgaac aggtcgcggc ggccgtcgcc gtcgtagtcg aggaagtgca
    10141 ccccccagcc gagcgacggc atggtcgccg gcccgacgcc gctctcgagc gccacgtcga
    10201 ggaacagcag ccctccttcg ttgtggtaga cggtcgtgta gtcgtgcgag aagttcgtgg
    10261 tcacgagatc gaaccggccg tcgttgtcgt agtcgccggc gtccaccccc atcgaggcct
    10321 gcgtcctgcc gtcctcggcg taggcgacgc cgctggtcgt ggcgacgtcc ttgaagcggc
    10381 cgccgtcgtt acggaacagg tagttcggcg tcgagtcgct ggcgacgaac aggtcgaggt
    10441 cgccgtcgtc gtcgaggtcg ctccagaccg cccccagccc gtagccgggg taggcggcgt
    10501 tcacccccgc cgcggcggtg acgtcgcgga accggccgcc gagattccgg aacagctgac
    10561 cggcctgcgg cttgagaccg cgcggaccgc acatcaccgg atagccgcgg tagttgcagt
    10621 tcggcccgct gccggtcggc ggcaggggag ggctgttctg gtcgtagtcg acgtagtgcg
    10681 agacgtacag gtcgatccac ccgtcgcggt cgtagtccgc gaaggcggcg ccggtgctcc
    10741 agccgcgccc ccctgcgagc ccggccgcgg cggtgacgtc gccgaagcgc agccggtcgg
    10801 tctgccgcag gagccagtag cgcccgaacc cggtgacgaa cagatcggtg cgcccgtcgg
    10861 cgtcgtagtc ggcggcggcg caaccttgcg cccagtaccc cgggagcgcg gtccccgatc
    10921 cctcggtgcg atcctcgaag gtcccgtcgc cgcgggcgcg gtagagggcg gagctggcct
    10981 tggcgccgcc gcgcggctgg ccgttcggca ggaagatgtc gaggaacccg tcgccgtcga
    11041 aatcggcgag gcagacgccg ttgccgaccg tttcgttgat ggtgtccttc tccctgctgc
    11101 cgcttcgatt caggagcgtg actccggccg ccttcgcgat atcgacgaac aggaccggag
    11161 cggtggccgc ggcggccgca ggcggggttt cgggaagcgc ctctccggac aggagccgcg
    11221 ccgctgcgag gacgcccgcc agcgtcgcga ggccgggagc gacccggcgg cgccatcgtc
    11281 gccgtcgctc aatcaagggt ggaggtgccc tgggtccggt tgctgcgcag aaagtcgagc
    11341 gactcggcat ggcgggcgaa ctcggcctgc gcctcgtcgg gccggccgga gcgcaggagc
    11401 gcctgcgcca gcgcatagtg cgccttcggg ttgaccggat cgagctcgag cgatcggcgc
    11461 agatcggaga tcgccggctc gagcgcaccg tcgtcggcgc tcatcgtccc gaggtagaac
    11521 catggatacg gatcggacgg cgacgcgtcg cgccccgcct cgaacgcggc gcgcgcctcg
    11581 tcgcgccgcc ccatcaggag cagcgtcttc cccagctcca tgagggtcgc ggaccgcgtg
    11641 ggatcgatgt cgagcgaccg ccggtaggcc gcgacggcgc ctacgtagtc gagcccctcg
    11701 cgcgcctgcc gcgcctgctc ggcggggagg gcggcgtcct tcagcccccg cgcgaccgcc
    11761 gcgtcgagga gcgctttggc gtccggcgcg tggccgtaag ccccgagcgc gcgggcgtgc
    11821 gtcaccacga ggtcggcgtc gtccggcgtc agctcaaggg cgcggcgctg ctgcgcgagt
    11881 ccctcctcga tccgtccggc gcggagggag atctccgcca gggcgcggta cgcccgggcg
    11941 ttcccggcgc cgtccgcggt cgcgcggcgc agccgatcct ccgcctgatc cgtctgaccg
    12001 gtctggaagt agaagaggcc cagatcggtc agcgtctccg tgtccgcggg atcgatcgcc
    12061 agctccttct cgaactggtt caccgcctgc gcgaagcgac cggtgcgcgc caggatctgt
    12121 cccatcgccc ggtgcacccc cgttcgccgc ggctccgcct gggcgagttg acggagcgcg
    12181 ctgagcccct cctcctcgcg tcccgtctgg gcgccgacga gcaccgcccg gtacagccgc
    12241 gcctcgaaca ggccggggtt cttctcaagg agcgtgtcca ccttcttcat cgcggcttcg
    12301 gaccgccccg cgtcctggag gcgacgggct tcggccaggg cgtgttcggg ggtctcggaa
    12361 caggcgatcg cggctgcggc gccgagcgcg acgatcgcgg cgcgcgacac gcgggaggtc
    12421 ggtaggaaca tgaagggcga tggtaaacga aaacccccgg gggttcaccc cgggggtcat
    12481 gtgtggtcct cgtcgtgcga ggagggaggt tcagaccttc gagacgttgg ccgcctgaag
    12541 acccttcggt ccctgcgtca cttcgaactg gaccgactgg ccctcgtcga gggtcttgta
    12601 gccttcaccc gcaatggccg agaaatgaac gaagatgtcc gtcccgtcct cctgctggat
    12661 gaaaccgtac cccttgctgt tgttgaacca cttcacttta cctgtaggca ctgaacccct
    12721 ctcctttcga ttcatcgcgg cgatggatcg cgcgcgccgc ccgcgctcat tcgtcgagca
    12781 aaaaaaaagc cacaggaaaa cggcttcctg tggtctcgac gcattcaagc gatagaacga
    12841 actcctgaag ccaatgtaca acgcaaattg tatgcacagg cagtgtatcc gtcaacatct
    12901 ttttcccgca aatctcgtgg tccggcgcgg ggaacgtgtc gtgtcggggg aaaaacggag
    12961 gtcagactcc ggggtcgtac ggagcacgct ccggcgcgcg caggagattc cgtttgcgct
    13021 cgcgcgtcgt ctgcacgtcc cccatcacct ggcggaggat gaccattccg ccctcgcgat
    13081 tcccgccctc gaactcgcgc gccgccatcc gcacctggcc tt
//
LOCUS       DASRIQ010000178         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1377094, whole genome shotgun sequence.
ACCESSION   DASRIQ010000178 DASRIQ010000000
VERSION     DASRIQ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377094"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFD06_04505"
     CDS             complement(<1..316)
                     /locus_tag="VFD06_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN02828.1"
                     /translation="MAAARYDAIIVGGGHNGLVAAGYLAKARFKVLVLERRPIVGGAC
                     VTEELIPGHKVTRTSYVCSLLMPEIIRDFRMADYGFQTLIPEPHSFYTYPDGRYMLWW
                     ADL"
     gene            614..2056
                     /locus_tag="VFD06_04510"
     CDS             614..2056
                     /locus_tag="VFD06_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZN02829.1"
                     /translation="MVPEVRILSDEEVRRLHTRALEILERVGIRYESPRALDVLAEAG
                     QEVDREAGVARLKPEFVEACIRSTPRVITLAGRLPEHDMTLEGKRIRITTNGQATFTQ
                     DFTTNLRRAATVKDLRESTKVAHYLDVVDYVWPMVVPTDVPGGSRGLHEMYHSFAMTS
                     KHIQHEIQLPEHVPFALEMLEVLCGDREAIRRRPPFSVVACTVSPLQHEPKMADLCID
                     LARAGIPIIIWPMPLTGATAPVTVPGTCLMSIVEFLSGVALFQAAAPGCPLIFPLGPE
                     VLDMKSGMAVCAGPEIPLFNLVLGQMAKFYDIPAIAVGLNSDAKEPGIQAAYESMQTG
                     LAAALSGADLLVGIGILDDNNVMSLPQMVIESEIARMVKRIREGVVISDETMMSHLIE
                     KAGPGSHFLGFKETLAGLKRGDVFFPRLSYRNTYDKFFEEGRNEVVYARSEVQRLLAQ
                     PDPDPLSLETDRELRRILDAADRACADSAA"
BASE COUNT          344 a          760 c          710 g          327 t
ORIGIN      
        1 cgaggtcggc ccaccacagc atgtaacggc cgtccgggta ggtgtagaac gagtgcggct
       61 cggggatgag ggtctggaag ccgtagtcgg ccatccggaa gtcgcggatg atctccggca
      121 tcaggagcga gcagacgtac gacgtgcgcg tgaccttgtg ccccgggatg agctcctcgg
      181 tgacgcaggc gccgccgacg atggggcggc gttcgaggac gagcaccttg aagcgggcct
      241 tcgccaggta gcccgcggcg acgaggccgt tgtgcccccc gccgacgatg atcgcgtcgt
      301 agcgtgccgc cgccatgccc ccctcctccc gccgcgccgc gcgcggcctg gcttcgttcg
      361 aatcccggaa actctgatgc tgtggcgcca atctagcaca gcgaccgcgg cgccgaaccg
      421 cgacgcgccc cagcgatgct gttacggctt gaggggaggt ggatcggcgc aactccaaag
      481 ccgtaacgcc attacggcgg tttcagggac cggaacgggt cgatcgggag ggggcgaccg
      541 gggcataatc ggccttgtca cggcagcatc tacgggcgcc cgcggcgcgg cgtccagagc
      601 tttgaggtga gacatggtcc ccgaggtccg gatcctgagc gacgaagaag tgcggcgcct
      661 ccacacccgc gcgctcgaga tcctcgagcg ggtcggcatc cgctacgaga gcccgcgcgc
      721 cctcgacgtc ctggcggagg cggggcagga ggtcgaccgc gaggccggcg tcgcccgcct
      781 gaagccggag ttcgtcgagg cctgtatccg gagcaccccg cgcgtcatca cgctggccgg
      841 ccgcctcccc gagcacgaca tgaccctcga agggaagcgg atccggatca cgaccaacgg
      901 gcaggcgacc ttcacccagg acttcacgac caacctgcgc cgggccgcga ccgtgaaaga
      961 cctgcgcgag tcgaccaagg tcgcccacta tctcgacgtc gtcgactacg tctggccgat
     1021 ggtggtgccg accgatgtcc cgggcggaag ccgggggctg cacgagatgt accactcgtt
     1081 cgccatgacc tcgaagcaca tccagcacga gatccagctc ccggagcacg tgccgttcgc
     1141 cctcgagatg ctcgaggtcc tatgcggcga tcgcgaggcg atccgccgcc ggccgccgtt
     1201 ctcggtcgtc gcctgcacgg tctcgccgct gcagcacgag ccgaagatgg ccgacctgtg
     1261 catcgacctg gcgcgcgccg gcatcccgat catcatctgg ccgatgccgc tcaccggcgc
     1321 gaccgctccc gtgaccgtcc cgggaacctg cctcatgagc atcgtcgagt tcctgtcggg
     1381 ggtggccctg ttccaggcgg cggcccccgg ctgcccgctc atcttcccac tcggtcccga
     1441 ggtcctcgac atgaagtcgg ggatggcggt ctgcgccggc ccggagatcc cgctcttcaa
     1501 tctcgttctc gggcagatgg cgaagttcta cgacatcccg gcgatcgcgg tcgggctcaa
     1561 ctccgacgcc aaagagcccg gcatccaggc ggcgtacgag tcgatgcaga ccggcctcgc
     1621 cgccgccctg tcgggagccg acctgctggt cggcatcggc atcctcgacg acaacaacgt
     1681 catgtcgctg ccgcagatgg tgatcgagtc ggagatcgcc cggatggtga agcggatccg
     1741 ggaaggggtg gtgatcagcg acgagaccat gatgagccac ctgatcgaga aggccggccc
     1801 cggctcccat ttcctcggct tcaaggagac gctggccggg ctcaagcgcg gcgacgtctt
     1861 cttcccccgg ctctcctacc gcaacaccta tgacaagttc ttcgaggaag gccgcaacga
     1921 ggtcgtctac gcccgctccg aggtccagcg gctcctcgcg cagcccgatc cggacccgtt
     1981 gtcgctggag accgaccgcg aactacggcg catcctcgac gccgccgatc gggcgtgcgc
     2041 cgactccgcc gcctagtcgg accgcttcac acggagccgc gggccgctcc ctccgccgcc
     2101 tcccgacatg agggttgcct ccgcccttct tcccggattg g
//
LOCUS       DASRIQ010000179         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3192102, whole genome shotgun sequence.
ACCESSION   DASRIQ010000179 DASRIQ010000000
VERSION     DASRIQ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3192102"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFD06_04515"
     CDS             <1..246
                     /locus_tag="VFD06_04515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN02830.1"
                     /translation="IGGRLLEALLEFGAGEGCVEATLEVRPSNVPALRMYTRAGFRVI
                     GRRRGYYSDTHEDALVMSCPLTPETAPPRLAGSDPGC"
     gene            323..571
                     /locus_tag="VFD06_04520"
     CDS             323..571
                     /locus_tag="VFD06_04520"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HZN02831.1"
                     /translation="MLHNHEDLRRELAQSNPEFRRLLDEHARYQTRLAELQGKAVLED
                     NERMETVNLKKQKLHVKDQMERILQEHIEKMSGSGVRH"
     gene            657..1283
                     /locus_tag="VFD06_04525"
     CDS             657..1283
                     /locus_tag="VFD06_04525"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HZN02832.1"
                     /translation="MPVAVQALPYLAALAAVAILAGWLGGTWAAAPFLAAAGFVLYFF
                     RDPQRVSPEGEGLVVSPADGRVTDVERRPEGARISIFLSLFNCHINRAPVAATIVSVT
                     HTPGLFRAAWDRRAATENERNHLQLRAADGEYGVTQIAGILARRIVCSKRAGDAVGRG
                     ERIGLIQFGSRTDLHLPPGVEPAVEVGDRVRGAVTVLARRVRAARVAS"
     gene            1280..2035
                     /gene="pssA"
                     /locus_tag="VFD06_04530"
     CDS             1280..2035
                     /gene="pssA"
                     /locus_tag="VFD06_04530"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HZN02833.1"
                     /translation="MKASGRLRRGAYLIPSLFTTGNLLCGYIAVVRSLKGEYEWAAVA
                     IFVAALLDRLDGWVARLTRTSSEFGVQLDSIADIVSFGVAPAFLVYLWGLAHLPKPWS
                     LAPFLYLAAGATRLARFNIQAPVQDRRFFVGLPIPAAACVIAACIFYSPARLVDPLTS
                     ILVSLLVVVLGVLMVSRLRYRSFKEIDLRSRIPWAMVALMAVAYFVVAASPQIVALAL
                     SFLYVLSGLRPRRSPAARPEARPHPAGGSHAGG"
     gene            2022..3062
                     /locus_tag="VFD06_04535"
     CDS             2022..3062
                     /locus_tag="VFD06_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HZN02834.1"
                     /translation="MRVVEPSAARGAHLAMFDAQTLVAKGVRDHLVGRAFPIASMRFF
                     SSRPDPEVNLTEFAGEAMFVSEPDPEALGPLDIAFFCGSRPDGERYLAWPRQRGFTAI
                     DLTSAARPQNDEPPINVAVNADRLPSEPRVVATPHPIALMLSTVLAAAHRGCGLKGAV
                     VTALQPVSEAGEEGINELYRQTLGLLNFQEWPQEVFGRQLAFNLVPAFAYGRGPVPGR
                     IDAREIEEEVRLIAGPGFDLSIGAVLAPVFHCHAAMVRLVLAPGRRPAELAAALAASA
                     DIKLDAAAARATPVDRAGQAGVLVAGPWPAGAPDACWIWVVSDNLQAGAALNAVRIAE
                     ALWRRDVPKGEA"
     gene            3059..3817
                     /locus_tag="VFD06_04540"
     CDS             3059..3817
                     /locus_tag="VFD06_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02835.1"
                     /translation="MRRIVAAVFAALLAAAGPGAAAQDLKRPEEQLAALYALRVQLEV
                     EQKRLDDDLLKHQRTAEQRESARGRLRQLYDELDDIVEGKTQSTPEELDQREAAVGRA
                     EQELAALTLAARDLRIRIRDAQERIALLQDRAGRLRRTLPSDNETLTGTWDVTYLPSN
                     DKGSFTLRQSGTIVAGEYVLEGGWRGSMQGTFVNGKILLHRIDSKLGHSQDLEGSIAP
                     DGKTLRGRWQNFILSGGQATSGDWVAQKRDRGDS"
     gene            3821..>3948
                     /locus_tag="VFD06_04545"
     CDS             3821..>3948
                     /locus_tag="VFD06_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HZN02836.1"
                     /translation="MGFPLIVARRYLFARRKQAIVFVISTISVLGVIVGVAALVVVL"
BASE COUNT          528 a         1383 c         1428 g          609 t
ORIGIN      
        1 atcggtgggc ggctgctcga ggcgctcctg gagttcgggg cgggggaggg gtgcgtggag
       61 gcgacgctcg aggtgcgccc gagcaacgtc cccgccctgc ggatgtacac ccgggcggga
      121 ttccgggtca tcgggcgtcg ccgcggctac tacagcgaca cgcacgagga cgcgctcgtc
      181 atgtcctgcc cgctcacccc ggagacggcg ccgccgcgcc ttgcagggtc ggatccggga
      241 tgctagaatt ttgggcagat gtcggccttc gaaggacgct cgcacttcgc ccgcaacccc
      301 aaagcgcagg gaggtctgaa cgatgctgca caaccacgag gatctcaggc gggagctcgc
      361 ccagagcaat ccggagttcc ggcggctgct cgatgagcat gcgcgctacc agacgcgtct
      421 cgcggagctc caggggaaag cggtcctcga ggacaacgag aggatggaga cggtcaatct
      481 gaaaaagcag aagctgcacg tcaaggatca gatggagcgg atcctgcagg agcacatcga
      541 gaagatgagc ggctccggcg tgcgtcactg acgtcgaact ccccgatcgg gcctcccgcc
      601 gacatcgcgc cacccgggcg gtccctcccc cctcgccttc tggagtcttg gagccgatgc
      661 cggtcgcagt tcaggctctc ccctatctcg ccgcccttgc tgccgtggcc atcctggccg
      721 gctggctggg agggacttgg gcggcggcgc cgttcctcgc ggccgccggc ttcgtcctgt
      781 acttcttccg cgatccgcaa agggtcagcc ccgaagggga aggactcgtc gtgtcgccgg
      841 cggacggccg ggtcaccgat gtcgagcgcc gcccggaggg ggcgcggatt tccatcttcc
      901 tgtccctgtt caactgtcac atcaaccgcg cgccggttgc cgcgaccatc gtctcggtga
      961 cccacacgcc cgggttgttc cgcgcggcgt gggaccggcg ggcggccacc gagaatgagc
     1021 gcaatcatct gcagctccgc gccgccgacg gggaatacgg cgtgacccag atcgccggaa
     1081 tcctggcgcg ccgcatcgtc tgctccaagc gcgccggcga cgcggtcgga cggggggagc
     1141 gcatcgggtt gatccagttc ggttcgcgca ccgatctgca ccttcccccc ggcgtggagc
     1201 cggcggtgga agtgggggac cgggtgcgcg gcgccgtgac cgtcctggcg cgccgggtcc
     1261 gcgcggcgcg ggtggcatcg tgaaggcctc ggggcgcctg cggcgcggcg cttacctgat
     1321 cccgagcctg ttcaccaccg gcaacctgct gtgcggctac atcgcggtgg tgcgcagcct
     1381 gaagggggaa tacgagtggg cggcggtggc gatcttcgtg gccgcgctgc tcgatcgcct
     1441 ggacggctgg gtcgcccgcc tgacgcgcac ctcgagcgag ttcggcgtgc agctcgactc
     1501 gatcgccgac atcgtctcgt tcggggtggc ccccgcgttc ctcgtgtacc tgtgggggct
     1561 ggcgcacctg ccgaagccgt ggtcgctggc gccgttcctc tacctcgcgg cgggggcgac
     1621 gcgcctcgcg cgcttcaaca tccaggcgcc ggtgcaggat cggcgcttct tcgtcgggct
     1681 gccgatcccg gcggcggcct gcgtgatcgc ggcctgcatc ttctacagcc cggcgcgcct
     1741 cgtcgatccg ctcacctcca tcctcgtgtc gctcctggtg gtggtgctcg gcgtgctgat
     1801 ggtcagccgg ctccgctacc gctccttcaa ggagatcgac ctgcgcagcc gcatcccctg
     1861 ggcgatggtc gccctgatgg cggtcgcgta tttcgtggtg gccgcgtcgc cgcagatcgt
     1921 cgcgctggcg ctgagcttcc tctacgtgct ctccggcctg cgcccgcggc gttccccggc
     1981 cgcccggccc gaggcgcggc cccaccccgc gggaggcagc catgcgggtg gttgagccgt
     2041 ccgcggcgcg gggggcgcac ctggcgatgt tcgacgccca gacgctcgtc gcgaaggggg
     2101 tccgggacca cctcgtcggt cgcgccttcc cgatcgcctc gatgcgtttc ttctcctcgc
     2161 gtcccgatcc ggaggtcaac ctcaccgagt tcgcgggcga ggcgatgttc gtctcggagc
     2221 cggacccgga ggcgctgggg ccgctcgaca tcgcgttctt ctgcggctcc cggccggacg
     2281 gggagcgcta tctcgcctgg ccccgtcagc gcggcttcac cgccatcgac ctgacctcgg
     2341 cggcccgccc gcagaatgac gaaccgccga tcaacgtcgc agtcaacgcg gaccggctcc
     2401 cgagtgagcc gcgcgtggtg gcgacgccac acccgatcgc gctgatgctc tccaccgtcc
     2461 tcgccgccgc gcaccggggc tgcgggctga agggcgccgt cgtgaccgcg ctgcagccgg
     2521 tgtcggaggc gggggaggag gggatcaacg agctgtaccg ccagacgctc ggcctgctca
     2581 atttccagga gtggccgcag gaggtgttcg gccgccagct ggcgttcaac ctcgtgccag
     2641 cgttcgcata cggcaggggc cccgttccgg ggcgcatcga cgcgcgggag atcgaggagg
     2701 aggtccggct catcgcagga cccgggttcg atctgtcgat cggggcggtg ctcgcgccgg
     2761 tcttccactg ccacgcggcg atggtgcgtc tcgtgctggc gccgggccgc cgtcccgccg
     2821 agctggccgc cgccctggcc gcatcggccg acatcaagct ggacgcggcc gccgcgcgcg
     2881 ccacgcccgt cgaccgcgcc ggccaggcgg gcgtgctcgt cgccgggccg tggccggcgg
     2941 gagcgcccga tgcctgctgg atctgggtcg tcagcgacaa cctgcaggcc ggggccgcgt
     3001 tgaacgcagt gcggatcgcc gaggcgctgt ggcggcggga cgtcccgaag ggggaggcat
     3061 gaggcggatc gtggcggccg ttttcgcggc cctcctggcg gcggccggac cgggggcggc
     3121 ggcccaggac ctgaagcgcc ccgaagagca gctcgcggcg ctgtacgccc tgcgggtgca
     3181 gctcgaggtg gagcagaagc gcctcgatga cgatctgctg aagcaccagc gcacggcgga
     3241 gcagcgggag tcggcgcggg gccggctccg gcagctgtat gacgagctcg acgacattgt
     3301 ggaggggaag acccagtcca cccccgagga gctggaccag cgcgaggcgg cggtcggacg
     3361 cgccgagcag gagctcgccg cgctgacgct cgccgcgcgc gacctccgga ttcgcatccg
     3421 cgacgctcag gagcgcatcg cgctcctcca ggaccgggcc ggccggctga ggcggaccct
     3481 cccgagcgac aacgagaccc tcaccgggac gtgggacgtc acctacctgc cgagcaacga
     3541 caagggctcg ttcacgctgc ggcagtccgg aaccatcgtc gccggcgagt acgtgctcga
     3601 ggggggatgg cgcggcagca tgcaggggac gttcgtcaac ggaaagatcc tgctgcaccg
     3661 catcgactcc aagctggggc actcgcagga tctcgaaggt tcgatcgccc cggacgggaa
     3721 gacgctgcgc ggccgctggc agaacttcat actctccggc gggcaggcga cctccggcga
     3781 ctgggtggcg cagaagcggg atcgcggcga ctcctgatcg atggggttcc cgctcatcgt
     3841 cgcccgccgc tacctgttcg cccgccgcaa gcaggcgatc gtcttcgtca tctccaccat
     3901 ctcggtcctc ggcgtcatcg tcggggtcgc ggccctggtc gtggtcct
//
LOCUS       DASRIQ010000180         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3734245, whole genome shotgun sequence.
ACCESSION   DASRIQ010000180 DASRIQ010000000
VERSION     DASRIQ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3734245"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFD06_04550"
     CDS             <1..189
                     /locus_tag="VFD06_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HZN02837.1"
                     /translation="VARWIPEIARLPPEHRHAPWLAPARVLEEAGVALDDDYPRPIVE
                     HASARERALVAFASIKAR"
     gene            complement(434..1120)
                     /locus_tag="VFD06_04555"
     CDS             complement(434..1120)
                     /locus_tag="VFD06_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreH"
                     /protein_id="HZN02838.1"
                     /translation="MLAVLGLGFILGLRHALDADHIAAVGAMVSERPGWRTALRTGLL
                     WGLGHSIALLVAGAFVIGLHLVIPDAIARALEAGVALMVLLLGGAALARALRSRSDVH
                     SHYHAHGRLVHRHLHFHDTSGAHDPHRIACVGIKPLLVGTLHGLAGSAALTLLVLAQI
                     PSATGGLAYLAVFGAGSIGGMALLSVAIGLPFAATLTRPGWNLALRVTAGAAGVLFGI
                     AYAWNQLGSA"
     gene            1179..1703
                     /locus_tag="VFD06_04560"
     CDS             1179..1703
                     /locus_tag="VFD06_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN02839.1"
                     /translation="MRRIILAATCIAMTGAGIAMSEMSAETTPAPTAAATVPGTGTVA
                     YVPGGEPRDAGLRRAIEDYVGRYRADRLADWKRLLHERLSVADPRPDGSIRFRGREDF
                     FATQQDFFATGRKIGERLEAVRVEEGRRIARVSAEFVFVEEGEERRGRLGLHFAEAGG
                     AWSVVAILFSYDRA"
     gene            complement(1732..2292)
                     /locus_tag="VFD06_04565"
     CDS             complement(1732..2292)
                     /locus_tag="VFD06_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02840.1"
                     /translation="MPRFAPRRHASRTAAPRAPAALGAVLMLVLATPARALAPVEVPP
                     GPVKQRVLSIGGWAGEPALGVSVKMLTSHELLDAWEAGFGWSQAAEDGWQFHGQRQWH
                     LKTLRKTPEGVLTFYLGAGGRVKHAEGTRFGLRGSAGLNYVFPNVERRWEAFLEIAPI
                     VDITPDVHSFVNAEIGLRYFLPFPTR"
     gene            2332..>3072
                     /locus_tag="VFD06_04570"
     CDS             2332..>3072
                     /locus_tag="VFD06_04570"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN02841.1"
                     /translation="MHYTVLPPSPALRSAVECFWRLEGIGAGLPAETIVPDGCMEIVL
                     HLGDRFEQVAPCGTVAPQPRAFVVGQMLRPLVVRPGARVETWGIRFRPGGAAPFFRAG
                     MQELTGRVVGLESLWGGDGERFAGRLFDARTVPARRAVLEAILLVKRKAPADPLATAA
                     ARAIVATRGVARTSVLAARAGVGRRQLERRFLRGVGIPPRRLARIVRFQHLFRLVDGR
                     DGANGAWADLACAAGYYDQAHLLRDFREF"
BASE COUNT          429 a         1072 c         1158 g          413 t
ORIGIN      
        1 gtcgcccgat ggatcccgga gatcgcccgt ctgccgccgg agcatcgcca cgccccctgg
       61 ctcgcccccg cgcgtgtcct cgaggaggcc ggcgtcgcgc tcgacgacga ctacccgcgg
      121 ccgatcgtcg agcacgcctc agcgcgcgag cgggccctcg tcgccttcgc atccatcaaa
      181 gctcgatagc gtcgggaatc cgcacgtttg ggaggcggcg gagggactgg gttgcgggga
      241 ccgcccgcgc gcgtgctgaa tcgcggccaa cgacgcgcgc gggctccggc cgcggattcg
      301 ctcgtctgcc gaaactcgag atggccctcg tgcgtccgcg gcaggccccc gcaacccagt
      361 ccctccgccg ccgcacaccc gaggccgtcc ggcgcttcaa accttgaatg cgacggctag
      421 gtgcggttac ttttcaggct gacccgagct ggttccaggc gtaggcgatt ccgaagagga
      481 ctcccgccgc gcccgccgtc acgcgcagcg ccaggttcca gccgggacgg gtgagggtgg
      541 cggcgaacgg caggccgatg gcgacgctca ggagtgccat gccgccgatc gagccggcgc
      601 cgaagacggc gaggtaggcg aggccgccgg tggcggacgg gatctgcgcc agcacgagga
      661 gcgtgagcgc cgccgacccg gcaaggccgt gcagcgtgcc gacgaggagc ggcttgatgc
      721 cgacgcaggc gatgcggtgc gggtcgtgcg cgccgctggt gtcgtggaag tgcaggtgcc
      781 ggtgcaccag ccggccgtgc gcgtggtagt gcgagtgcac gtcggatcgg gagcggagcg
      841 cgcgcgcgag cgccgcgccg ccgaggagca gcaccatgag ggcgacgccg gcttcgagcg
      901 cccgcgcgat cgcgtcgggg atgaccaggt gcagtccgat cacgaacgcg ccggcgacga
      961 ggagcgcgat ggagtggccg aggccccata gcaggccggt ccgcagcgcc gtgcgccagc
     1021 cgggacgttc gctgaccatc gcgccgaccg ccgcgatgtg atcggcgtcg agtgcgtggc
     1081 gcagaccgag gatgaaaccg agccccagaa ccgccagcat gcgcgggcat catatataag
     1141 gatgcggcgg acaggaagtc cgcggcggag gagacggcat gaggcggatc attctggcgg
     1201 cgacgtgcat cgcgatgacc ggagcgggga tcgcgatgag cgagatgagc gcggagacga
     1261 caccggcgcc gacggcggcg gcgaccgtcc cggggacggg aaccgtcgcc tacgtcccgg
     1321 ggggggagcc gcgcgacgcc gggctgcgcc gggcgatcga ggactatgtc ggccggtacc
     1381 gcgccgatcg cctcgccgac tggaagcgcc tgctgcacga gcgactgagc gtcgccgatc
     1441 cgcgccccga cggcagcatc cgcttccgtg ggcgcgagga cttcttcgcc acgcagcagg
     1501 acttcttcgc caccggccgg aagatcggcg agcggctgga agcggtgcga gtggaggagg
     1561 ggcgccgcat cgcgcgggtc tcggcggagt tcgttttcgt ggaggagggg gaggagcgcc
     1621 gcggtcgtct cggcctccac ttcgcagagg cgggcggagc gtggagcgtc gtcgccattc
     1681 tcttctcgta cgatcgggcc tgaaccgccg ctctcccggt gggggcgtgg gctaacgggt
     1741 ggggaatggc aggaagtagc gcaggccgat ctcggcgttc acgaaggagt ggacgtcggg
     1801 ggtgatgtcg acgatcggcg cgatctcgag aaacgcctcc cagcgccgct cgacgttcgg
     1861 gaagacgtag ttcagccccg ccgatccgcg cagaccgaag cgggtcccct cggcgtgctt
     1921 cacccgtccc cccgccccca ggtagaaggt cagcactcct tcgggcgtct tgcgcagcgt
     1981 cttgagatgc cactggcgct ggccgtggaa ttgccagccg tcctccgccg cctgcgacca
     2041 gccgaacccc gcttcccagg catccaggag ctcgtggctc gtcagcatct tcacgctgac
     2101 gccgagcgcc ggctcgccgg cccatccacc gatcgagagg acccgctgtt tcaccgggcc
     2161 cggcggcacc tcgaccggcg ccagggcgcg ggccggggtc gcgagcacca gcatcaggac
     2221 ggcgccgagc gcggccgggg ctcggggagc ggccgtccgc gatgcgtgac gcctcggcgc
     2281 gaagcgcggc atggagggcg gattatacgg gagcggccct aagatacgcg catgcactac
     2341 acggtgcttc ccccctcccc cgcgctgcgc tccgccgtcg agtgcttctg gcgcctcgag
     2401 gggatcggag ccggccttcc ggccgagacg atcgtgccgg acggctgcat ggagatcgtc
     2461 ctccacctgg gggatcggtt cgagcaggtc gctccctgtg ggaccgtcgc gccgcagccg
     2521 cgcgccttcg tcgtcgggca gatgctccgg ccgctggtcg tccgccccgg cgcgcgcgtc
     2581 gaaacctggg ggatccgctt ccggccgggt ggcgcggcgc cgttcttccg cgcggggatg
     2641 caggaactga ccggacgcgt cgtcggtctc gagtcgctgt ggggcgggga cggagaacgc
     2701 ttcgccgggc ggctcttcga cgcgcgcacc gtcccggccc gccgcgccgt cctcgaggcg
     2761 atcctcctgg tgaaacgaaa ggcgccggcc gatccgctcg ccaccgcggc ggcccgcgcc
     2821 atcgtcgcga cgcgcggcgt cgcgcgaacg tccgtcctcg cggcgcgcgc cggagtcggg
     2881 cggcgccagc tcgaacggcg cttcctgcgc ggcgtcggga tcccgccgcg caggctcgcg
     2941 cgcatcgtcc ggttccagca cctcttccgg ctggtggacg ggcgggatgg agccaacggg
     3001 gcgtgggccg atctcgcctg cgccgccggc tattacgacc aggcccacct gctgcgcgac
     3061 ttcagggagt tc
//
LOCUS       DASRIQ010000181         7534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3734638, whole genome shotgun sequence.
ACCESSION   DASRIQ010000181 DASRIQ010000000
VERSION     DASRIQ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3734638"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2043
                     /locus_tag="VFD06_04575"
     CDS             <1..2043
                     /locus_tag="VFD06_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02842.1"
                     /translation="GSPISRSARVLILLVGGAIALAAWVGWRVARDPAFPFLTHDAAA
                     EWILFPAPPSTLAHPGEEVETAFRASFDLASVPARATLRARCFHAGDATLNDTPVDVP
                     CAGETDWKRTVEGDVARALRPGANVLSVRVRSRFGPPALWLSIEGTGIRAATGARWEA
                     SQAGSPWRPARLATTPMDRWELETPGGATGTPSVADPTVLEAARHSLPAWIAFAAACG
                     ALLLAVRRLPQWLASLEGGSAAEVERLVAPILAGFAALLWCMLVWNNLWVVLSWSGEP
                     GVAPPLGFDLSSHLEYIQYILERGALPLAHEGWEMYQPPLYYLMASGLLACFGQVTLD
                     QAMAILRALNWAGGLLLVGALYESLRLLHPNETRRVLRGFLLGAFLPAQLYMTHYVSN
                     EIWSAALASASIAVAIRILVRSPDSIGLHLLLGALMGAALLTKFSTVMVVAVIVAVLV
                     GRRVVDGGSSTVIALRTVGGMLAACAAVCGWHFLRVALRFGNPLIGNWDPQIGYTWWQ
                     DPGYHTAGYYTGFGAALHRPVYSAFHSFADAFYSTLWGDGMIGGSALVEVRPPWDFGS
                     MTAGYLLAILPTVAILVGTVAALAQLVRAPRAEGWLVLGVLGATAYGVFYMTLRLPFY
                     AQAKAFYGLSAFIPLAVLGSLGLGVLAHRAPAAHAVFDVLLGVWAINAFVTFWIRL"
     gene            2191..3270
                     /locus_tag="VFD06_04580"
     CDS             2191..3270
                     /locus_tag="VFD06_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02843.1"
                     /translation="MAVARFRVLFPCCLLVTLAWAGFFSGEALAQPVATLALCGDGDG
                     NIGTNWFSLPTHTDMTTAEDLCLSIPGAVTVSQGVADSLPATSLRWVFDCATGACTSS
                     QTIPEPGCALSDCFCIDPGEGYEVITAADTPWDVNQCDRFDPIALPVGFQNYLVSIPF
                     DTFLVTANDLAEHVYLPNQGIVRGTVTGINCATGALTTCNAGTAGCDAFLLQPGRAYR
                     VRYTTPTTGISYTNPVSCVPAAPPGLAVCPIGDLSFTDDDTFTWTAPVACPLPLLYDA
                     IRGDLACLRGSCRQSIPALTPPCAGCLLLEDDDGADTTTVDAESPELGIGWWYHSRVD
                     GGTWRDPVAGASCTDRDIALAAGCP"
     gene            complement(3314..3389)
                     /locus_tag="VFD06_04585"
     tRNA            complement(3314..3389)
                     /locus_tag="VFD06_04585"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3354..3356),aa:Lys,seq:ttt)
     gene            complement(3452..4012)
                     /gene="rfbC"
                     /locus_tag="VFD06_04590"
     CDS             complement(3452..4012)
                     /gene="rfbC"
                     /locus_tag="VFD06_04590"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HZN02844.1"
                     /translation="MPFQILDRPLPGVVVLQPDAYADERGLFVESFRADRFLEMGLPA
                     TFVQENQSTSRRGVIRGLHFQWEPPMGKLMRVTRGRAFLVGVDIRKDSETLGRWFGLE
                     ASDEDRIQVWAPAGFARGFSALEDGTEVLYKCTGTYDPRHESGIRWDDPRIGIRWPVK
                     DPLLSQKDRAAQSLDDWLRRPESKHF"
     gene            4198..6930
                     /locus_tag="VFD06_04595"
     CDS             4198..6930
                     /locus_tag="VFD06_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02845.1"
                     /translation="MAFGGALLVSIGLAALNSVLPLRQTIRQEALDDLHDSATILLER
                     VEGYLGQRCADMRSMAALLADARVDAELGARLGRAQEAWDVPWLLLAAVDEHGRPLAL
                     SRPGTTFGSLLGPATALSDPTACSIVVRPEGRPLVLVEAVLGDPARQHRLRGLLDWAP
                     IEAMVRGAGVERAGPDSSAFLVLRDGVTGALLAGSWSHLAALQGSAEPAPGSDGEPRH
                     VPLPDGREFLVAHAGPSGTAPGIPPLRVTAVRDPSIALAKPDHRVGVLALAALVGLVL
                     AATLAYLIAGDLVERIERLGDAARRLAAGDRGAPDLAAGDDGREDEVGILARDFAAMS
                     RSIQRERASLEDAVARRTRELEQKNAALDHALSESRAGTRAKSEFLANVSHEIRTPLN
                     GILGMTALALDADLPPEARSHLTLVKRSADGLLSLVNDLLDFSRIESSHMRLEPIPFY
                     LRPCVEEVVRTMAPRAEEKGLDLGVRIDERIADDLVGDPGRLRQVLVHLLGNALKFTE
                     RGRVDLDVEPLREAPGETTLRFTIRDTGIGIDREKQALILEPFTQADGSSTRRHGGVG
                     LGLTIASELVTLMHGRITVESEPGRGSAFTFHVTFGRPRPQAQPSPGQPAPLVRVRAL
                     VVDDDPVNRRLLEARLVSWGMRPQCARDAEEAMALLQSAAGSGDLYQVALVDRRMPRQ
                     DGFALAARIKQDVRLATLPILLLSSDGERGDALRCRSIGIAAYLIKPVKDADLKDALT
                     TVLGASGAAVDRLVTRHALYEGRPRPPALEAAAEDGDAAAPSAPRPVPARDEPPAIDP
                     VDLIGRVEGDRLLLSDLVRAFLTTAPLQLRAIDAALERGEGSAIVRAANTLRGSIGTF
                     GALPAVQVLGRLEVLGGLGDLASARQVRSDLEREMDRLVRALRPFATENNECAS"
     gene            6918..7529
                     /locus_tag="VFD06_04600"
     CDS             6918..7529
                     /locus_tag="VFD06_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN02846.1"
                     /translation="MRILIAEDDLVSRKMLEATLSRWGYEVVVTCDGQGALDSLTGPD
                     APRVAILDWMMPSLDGVDVCRKVREGSVAQPPYIILLTAKGNKEDIVSGLEAGADDYV
                     IKPFDREELRARLQAGLRIITLQNQLAARVRELEEAIARIRTLQGLLPICSYCKRVRN
                     DGDYWQQVESYISDHSDARFSHGICPDCYESVVRPQLQTLKAG"
BASE COUNT          995 a         2755 c         2592 g         1192 t
ORIGIN      
        1 ggctccccga tctcccgcag cgcgcgcgtt ctgatcctcc tcgtcggggg cgccatcgcg
       61 ctcgcggcat gggtgggatg gcgggtcgcg cgggaccctg cgtttccctt cctcacgcac
      121 gacgccgcgg cggagtggat cctgtttccc gccccgccca gcactctcgc gcaccccggc
      181 gaagaggtcg agaccgcctt tcgcgcctcg ttcgacctcg cgagcgtccc cgcgcgggcg
      241 acgctgcgcg cccgctgctt ccatgcgggg gacgcgacgc tcaacgacac gccggtcgat
      301 gtcccctgcg cgggcgagac cgactggaag cgcaccgtgg agggggacgt cgcccgcgcc
      361 ctgcgaccag gagcgaatgt cctgtccgtc cgggtcagga gccggttcgg gccgccggcc
      421 ctgtggctgt cgatcgaggg aaccgggatc cgcgcggcga ccggcgcgcg ctgggaggcc
      481 agccaggccg gatccccctg gcgtccggcg cggctcgcga cgacgccgat ggatcgctgg
      541 gagctggaga cgccgggggg agccaccggc acgccctccg tggccgatcc caccgtcctc
      601 gaggccgccc ggcattcgct tccggcctgg attgccttcg ccgccgcgtg cggcgcgctt
      661 ctcctcgcgg tccgacgcct gccgcaatgg ctcgcctctc tggaaggggg atccgcggcc
      721 gaggtggaac gcctggtcgc gccgatcctc gccgggttcg cggccctgct ctggtgcatg
      781 ctcgtctgga acaatctctg ggtcgtcttg agctggagcg gcgagcccgg cgtcgcgccg
      841 cccctcggct tcgacctctc gagtcacctc gaatacatcc agtacatcct cgagcggggc
      901 gccctgcctc tcgcccacga gggctgggag atgtaccagc ctccgctgta ctacctcatg
      961 gcgtcgggcc tcctggcgtg cttcggacag gtgacgctcg accaggcgat ggcgattctt
     1021 cgcgccctga actgggcggg cgggctcctc ctcgtgggcg ccctgtacga gagcctccgc
     1081 ctcctccatc ccaatgagac gcggcgcgtc ctgcgcgggt tcctcctcgg tgccttcctc
     1141 ccggcgcagc tctacatgac gcactacgtc agcaacgaga tctggtcggc ggctctggcc
     1201 tcggcgtcga tcgccgtcgc gatccgcatc ctcgtgcggt cccctgattc gatcgggctg
     1261 cacctgctgc tcggggcgct gatgggggcg gccctcctca ccaagttctc gacggtcatg
     1321 gtggtggcgg tgatcgtcgc ggtcctcgtg gggcggcggg tcgtggacgg cggatcatcc
     1381 acggtgatcg ccctgcgcac ggtcggcggc atgctcgccg cctgcgccgc ggtctgcggc
     1441 tggcacttcc tgcgcgtcgc gcttcgtttc ggcaacccgc tgatcgggaa ctgggacccg
     1501 cagatcggct acacgtggtg gcaggacccc ggctatcaca ccgccgggta ctacaccggg
     1561 ttcggcgctg cgctgcaccg gccggtctac agcgccttcc attccttcgc cgacgcgttc
     1621 tactcgaccc tctggggcga cgggatgatc ggcgggagcg ccctcgtcga ggtccggccg
     1681 ccgtgggatt tcggctcgat gaccgccggc tatctcctgg ccatcctgcc gaccgtggcg
     1741 atcctggtgg ggacggtcgc ggcgcttgcg caactcgtgc gggcgccgcg ggccgaaggg
     1801 tggctggtgc tcggcgtcct cggcgcgacc gcctacggcg tcttctacat gacgctgcgc
     1861 ctccccttct acgcacaggc gaaggccttc tacggcctct ccgcgttcat ccccctcgcg
     1921 gtgctgggca gcctgggcct gggcgtcctc gcccaccgcg cgccggccgc gcatgcggtc
     1981 ttcgatgtcc tcctcggagt ctgggcgatc aacgcgttcg tcacgttctg gatccgtctc
     2041 taaggacgga gcacgaggat ctcagcaacc ttgctgtctt gacgcgatcg ggatttgacg
     2101 gtagaagaca gcccagctcg cctttccctc cggaacgtcc ggaagcgatc ggatcctcgc
     2161 cgcgcgccgc ctcatcctcg gaggtgccag atggctgttg ctcgtttccg cgtcctcttt
     2221 ccctgctgtc tgctcgtcac gctggcatgg gccggcttct tctccggcga ggcgctggcc
     2281 cagccggtcg ccacgctcgc gctgtgcggc gacggcgacg gcaacatcgg aaccaactgg
     2341 ttctcgctgc cgacccatac cgacatgacg accgcggagg atctctgcct ctcgattccc
     2401 ggggcggtca cggtttctca gggggtggcc gacagccttc cggccacgag cctccgctgg
     2461 gtgttcgact gcgccacggg cgcctgcact tccagccaga ccatccccga gcccggctgc
     2521 gcactgtccg actgtttctg catcgatccg ggggaagggt acgaggtcat caccgcggcg
     2581 gacacgccct gggacgtgaa ccagtgcgac aggttcgacc cgatcgcatt accggtgggc
     2641 ttccagaact acctcgtctc gatccccttc gacaccttcc tcgtgacggc caacgacctg
     2701 gcggaacatg tctacctgcc aaaccagggg atcgtgcgcg gcacggtcac ggggatcaac
     2761 tgcgcgaccg gggcgctgac cacctgcaat gcgggcaccg ccgggtgcga cgcgttcctc
     2821 ctccagcccg ggcgggcgta ccgggtgcgc tacacgacgc cgacgacagg catctcctat
     2881 acgaacccgg tgtcctgcgt tccggccgcc ccgccggggc ttgcagtctg tcccatcggg
     2941 gacctgtcgt tcaccgacga cgacacgttc acatggacgg cgccggtcgc gtgcccgctg
     3001 ccgctccttt atgacgcgat ccgtggggac ctggcctgcc tgcgtggctc ctgccgccag
     3061 agcatcccgg cgctgacgcc accgtgcgcc ggctgcctgc tgctcgagga cgacgacggc
     3121 gccgacacga ccacggtcga tgcggaatcg ccggaactgg ggatcggctg gtggtatcac
     3181 tcccgcgtcg acggcggcac ctggcgcgat cctgttgcgg gcgcctcgtg caccgaccgc
     3241 gacatcgcgt tggccgcggg ctgtccctag cgtccgaacg gtggccagtc tgttggagga
     3301 actcgaggaa gaatggtgag ccgtgctgga ctcgaaccag cgaccctgtg cttaaaaggc
     3361 acatgctcta ccgcctgagc taacggcccg accgattggc gcgagtctag gaggggctta
     3421 cgcaaatagc aagagcggcg ccgctcacac cttagaagtg cttcgattcc gggcggcgca
     3481 gccagtcgtc gagcgactgc gcggcacggt ccttctgcga caggagcggg tccttcaccg
     3541 gccagcggat gccgatgcgc gggtcgtccc agcggatccc cgactcgtgc cgcggatcgt
     3601 aggtgccggt gcacttgtac aggacctcgg tcccgtcctc gagggcggag aagccgcgcg
     3661 cgaaaccggc cggggcccac acctggatcc gatcctcgtc ggaggcctcg aggccgaacc
     3721 agcggccgag cgtttcggag tctttccgga tgtccacccc gacgaggaag gccctaccgc
     3781 gggtcacccg catcagtttc cccatcggcg gctcccactg gaaatgcagg ccgcggatga
     3841 cgccgcgccg cgaggtggac tggttctcct gcacgaaggt cgcaggcagc cccatctcga
     3901 ggaaccggtc ggcccggaac gactcgacga acaggccccg ctcgtcggcg taggcatccg
     3961 gctgcagcac gacgaccccc ggaagcgggc gatccagaat ttgaaagggc atgcgccggt
     4021 ctccgggcgg atgataccgt gcgggggcct aggcggaccg gcccggcgtc ggttcgttga
     4081 cacagggggc gtcccccttc ccccttcttg caccccttcc ccgcggtcct tacctttgtc
     4141 catcgcatgc gcgccgggtg gaggtctgcg tcttcgcggc tgttcacgcg tctcgccgtg
     4201 gcgttcggcg gcgcgctcct cgtctccatc ggcctcgcgg cgctcaactc ggtcctgccg
     4261 ctgcggcaga ccatccgcca ggaggcgctc gacgacctgc acgactccgc caccatcctg
     4321 ctcgagaggg tcgaggggta cctcggccag cgctgcgccg acatgcgttc gatggccgcc
     4381 cttctggccg atgcgcgcgt ggatgcggag ctcggcgcgc ggctcggccg cgctcaggag
     4441 gcttgggatg tcccctggct gctcctcgcc gccgtggacg aacacgggcg gcctctggcg
     4501 ctgagccgac ccgggaccac cttcgggagc ctgctgggcc ccgcgacggc tctctccgac
     4561 ccgaccgcct gctccatcgt ggtgcggccc gagggccgtc cgctcgtcct cgtcgaagcg
     4621 gttctcggcg atcccgcgcg gcagcaccga ctccgaggtc ttctcgactg ggccccgatc
     4681 gaagcgatgg tgcgcggcgc cggggtcgag cgcgccggtc ccgacagcag cgccttcctg
     4741 gtcctgcgcg atggggtgac gggggcgctg ctcgcgggct cctggtcgca cctcgcggcg
     4801 ctccaggggt ccgccgagcc ggctccggga agcgacggcg agccgcggca cgtccctctc
     4861 cccgacggcc gcgagtttct cgtggcccac gcgggtcctt cggggacggc cccgggaatc
     4921 ccgccgctcc gcgtgaccgc ggtgcgcgac ccgtcgatcg cgctcgccaa gccggatcac
     4981 cgcgtcgggg tgctcgccct cgccgccctc gtcggtctgg tgctggcggc gacgctggcc
     5041 tacctgatcg ccggcgatct ggtcgagcgg atcgagcgcc tcggagacgc ggcgcgccgt
     5101 ctcgcggcgg gcgatcgcgg cgcccccgac ctggccgccg gggacgacgg acgcgaggac
     5161 gaagtcggga tcctggcgcg cgacttcgcc gccatgtccc ggtcgatcca gcgcgagcgg
     5221 gcctcgctcg aggacgccgt ggcccggcgc acccgcgagc tcgagcagaa gaatgccgcc
     5281 ctcgaccacg ccctgagcga gtcgcgcgcc ggcacccgcg ccaagtcgga gttcctcgcg
     5341 aacgtcagcc acgagatccg cacgccgctc aatggcatcc tcggcatgac ggcgctggcg
     5401 ctcgacgccg acctcccgcc ggaggcgcgc tcccacctga ccctcgtcaa gcgatccgcc
     5461 gacgggctcc tgtcgctggt caacgacctg ctcgacttct cgcgcatcga gtcgtcgcac
     5521 atgcgcctcg agccgatccc gttctatctc cgcccgtgcg tcgaggaggt ggtgcgcacc
     5581 atggcgccgc gcgccgagga gaaggggctc gacctcggcg tccgaatcga cgaaaggatc
     5641 gcggacgacc tcgtcggaga tccggggagg cttcggcagg tgctggtgca cctcctcggc
     5701 aacgccctca agttcaccga gcggggccgg gtcgatctcg atgtggagcc gctgcgcgag
     5761 gctccgggcg agacgacgct gcgcttcacc atccgcgaca ccggtatcgg catcgatcgc
     5821 gagaagcagg ccctcatcct cgagccgttc acccaggcgg acggatcgag cacgcggcgc
     5881 cacggcggcg tcggcctcgg tctgaccatc gcctccgagc tcgtgacgct catgcacggg
     5941 cggatcacgg tggagagcga gccggggcgc ggcagcgcct tcaccttcca tgtgacgttc
     6001 ggccggccgc gtccgcaggc gcagccgtcg ccgggccagc cggcgccgct cgtccgggtg
     6061 cgggcgctcg tcgtggacga cgacccggtc aaccggcgcc tcctcgaggc ccgcctcgtc
     6121 tcgtggggga tgcggccgca gtgcgcccgc gacgccgagg aggcgatggc cctcctccag
     6181 agcgccgccg gctcgggcga tctctaccag gtggcgctcg tcgatcgccg catgccgcgc
     6241 caggacggtt tcgccctcgc ggcccgcatc aagcaggacg tccggctcgc caccctgccg
     6301 atcctcctgc tcagcagcga cggcgagcgc ggcgacgcgc tccggtgccg gagcatcggc
     6361 atcgccgcct acctcatcaa gccggtgaag gacgccgacc tcaaggatgc cctgaccacc
     6421 gtgctcgggg cgtccggggc ggcggtcgat cgcctcgtga cccgccacgc cctctacgaa
     6481 gggcggccgc ggccgccggc gctggaagcc gccgcggagg atggagacgc cgcggccccc
     6541 tccgcccccc ggccggtccc ggcgcgcgac gagccgccgg cgatcgatcc ggtggacctg
     6601 atcgggcggg tggaagggga tcgtctgctg ctgtcggacc tcgtgcgcgc cttcctcact
     6661 acggcgccgt tgcagctgcg cgccatcgac gccgccctcg agcgggggga ggggtcggcc
     6721 atcgtccggg ccgcgaacac cctgcgcgga tcgatcggga ccttcggggc gctcccggcg
     6781 gtgcaggtgc tgggacggct cgaagtgctg ggaggactgg gagacctggc gtcggcacgc
     6841 caggtccgga gcgacctgga acgggaaatg gaccggctgg tgcgggccct gcggccgttc
     6901 gccacggaga acaacgaatg cgcatcctga tcgccgaaga cgacctggtg tcgcgcaaga
     6961 tgctcgaagc caccctgtcc cgctgggggt acgaggtggt ggtcacctgc gacggccagg
     7021 gggcgctcga ctcgctgacc ggccccgatg cgccgcgcgt cgccatcctc gactggatga
     7081 tgccgagcct cgacggggtg gacgtctgcc gcaaggtgcg cgaggggagc gtggcgcagc
     7141 ctccgtacat catcctgctc accgccaagg gcaacaagga agacatcgtc tcggggctgg
     7201 aggccggggc ggacgactac gtcatcaagc cgttcgatcg cgaggagctg cgggcgcgac
     7261 tccaggccgg gctgcgcatc atcacgctgc agaaccagct ggcggcgcgc gtccgggagc
     7321 tggaggaggc gatcgcccgc atccgcaccc tgcaggggct gctgccgatc tgctcctact
     7381 gcaagcgggt gcgcaacgac ggcgactact ggcagcaggt ggagtcgtac atcagcgatc
     7441 actccgacgc ccgcttcagc cacggcatct gccccgactg ctacgagagc gtcgtccgcc
     7501 cgcagctgca gaccctcaag gccggctgag ccgc
//
LOCUS       DASRIQ010000182         5261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2148996, whole genome shotgun sequence.
ACCESSION   DASRIQ010000182 DASRIQ010000000
VERSION     DASRIQ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148996"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2052)
                     /locus_tag="VFD06_04605"
     CDS             complement(<1..2052)
                     /locus_tag="VFD06_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN02847.1"
                     /translation="MTQRQDPRNREHGFSLVEMLVASAVFAIVAAVVFIFYTAAQKSY
                     KSGENFSEQQQSTRVAFDRMISDMRLAGFNTNPDGDATRVDEQIEGAWDTAVTIRGDF
                     DFEDTSANASPEAALPGAVYNVVSTGNDEIVTYVLAKPGPVGSDSLTIRVDANRPRVK
                     AVQTVTIPNVVLVQNDPPYTLYRVTLRDVAGAFPGAPQAVTSFSYEPVADNIRTLTFQ
                     YMPDAGTVPLNANTPANTADDIGGGDATALTRSIIRKISVNLVGMTPDEDLDYVDASD
                     ATATTHYRKFDLSSDVNPENLGKTGVKDIDVTPPPAPTNISLVPGHCKGLLVKWDTPS
                     SSSGVTSYGLKYWPNGAPNPFSTAAVTYPHSEFGVVDFEGHGFVAGLNNGTSYCVQVQ
                     ARDVVGNQSGWAPGTAPCATVAEASTPDVPQNLTATGNGTLAPQDSQIVLTWNEVKNN
                     SNNVTNDPDLLNAHTILRDGKGYKLYRDTVSTFTPDDATNLVAPNTILGNGVLTWTDT
                     AVANCQTYYYKLKASDTCDVLSNASTGATGQASTLIAPNAPTGVTGARTSIQTVTLSW
                     TPVTTKVDGTTTFVNQYKVYRTKQPYGTILASLSSGAFSLLGVATSNTYVDNLAGADI
                     SDLNNRTQALYYQVAAADLCGNESTRSGGVDVACNIGNITVNPADGASASGMVPISLS
                     IS"
     gene            complement(2102..2578)
                     /locus_tag="VFD06_04610"
     CDS             complement(2102..2578)
                     /locus_tag="VFD06_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02848.1"
                     /translation="MRAIERNHRTSPDAGFSLIEVIIAIGVLAGVMLSIASMFIMGGR
                     QVKTGKTLTEATTLAQDLMEQFDKESFASLYLNLGAAATDSNRTVLSNVTGSPIVGWQ
                     TEIARKLNNGFASVRLDAMGPGTPNFGAAAGIKVTVRVTWNELGRPQSVALSTVRF"
     gene            complement(2594..3097)
                     /locus_tag="VFD06_04615"
     CDS             complement(2594..3097)
                     /locus_tag="VFD06_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN02849.1"
                     /translation="MTTERGKEAMKRRTEGFSLSELLVVIALMGIFILFGGPAMADAF
                     RAYKVRAAADIVATDVRAMRYSAVGQRGPRTMTINSQGAGTDPNTYTFVNAVGDTLLR
                     RVEQGVNIEDASADTLSFSITGATGSAGAEVIIVSMDINGTRGDRYTISVSPSGTVST
                     AFSSYTP"
     gene            complement(3294..5207)
                     /locus_tag="VFD06_04620"
     CDS             complement(3294..5207)
                     /locus_tag="VFD06_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02850.1"
                     /translation="MTPAFGRSYAGQIKIFLVLLVLFLAVAIYFDFHLLVIARNAIQE
                     EAGLRLGVEADLLRTELERDDMLRPASPTEATPFVPPAHLERLVRVHDLRRVDLLAID
                     GRVVASSDPARIGRSDPFLLRDGGRERRRVLAGATAVTPLDRAGRSPFATLAAYRPIR
                     NRAYETQAILRLEREVPALASVDLDLRSIAALQAGGLAFVLLLVLLFARWLLRPYRRL
                     QQAAGLAAVPDPSAGMDDAEALVTAVQGVLEKMRAQEGELLALKGSAERASGSAPGLP
                     GERLLSGMTSAALVFDGAGRLVTLNAAAEKLLSVGPQPPGRSASDLLAAHPPLLDLVR
                     GGLGRGEARSREVVTLRGRDGRPAHVGVMLSPIRGTGAGTGDAAIEGVVCLLTDLTEI
                     RALRERARLRDNLAILGEMSAGIAHEFRNALAVIQGHARLQSRALAQNPARGHAEAIE
                     REVARLLKVVGDFLRYARPTTPDLAETDLARLVREAVDDLRADPGNALVTVSVEGSFP
                     SLFADETLLRQALQNLLRNASEAVREAGGIVAPRTIVVRGREEPGEGGRVRIEVEDDG
                     PGIPPADRGHIFTPFYTTKQEGTGLGLPLVQKAAALHDGTVEALPREGGGTIMVLILP
                     LRPAPPVPIDLVA"
BASE COUNT          731 a         1794 c         1885 g          851 t
ORIGIN      
        1 gctgatgctg aggctgatcg gcaccatccc ggacgccgag gctccgtcgg ccgggttgac
       61 cgtgatgttg ccgatgttgc aggcgacgtc gacgccgccc gagcgggtgc tctcgttccc
      121 gcagaggtcc gcggcggcga cctggtagta gagggcctgc gtccggttgt tcaggtccga
      181 gatgtctgcg ccggccaggt tgtccacgta cgtgttcgag gtggcgaccc cgaggagcga
      241 gaaggctccg gaggacaggc tcgcgaggat cgttccgtac ggctgcttcg tgcggtagac
      301 cttgtactgg ttcacgaagg tcgtcgtgcc gtccactttg gtcgtgaccg gagtccagga
      361 cagggtcacg gtctggatgc tcgtgcgggc gcccgtcacc ccggtcgggg cgttcggcgc
      421 aatgagtgtc gacgcctggc cggtcgcgcc ggtcgaggca ttgctgagga cgtcgcaggt
      481 gtcgctcgcc ttgagcttgt agtagtaggt ctggcagttg gcaacggccg tgtcggtcca
      541 ggtcaggacg ccgttgccga ggatcgtgtt gggcgccacg agattcgtcg cgtcgtccgg
      601 cgtgaacgtc gagacggtgt cgcggtaaag cttgtagccc ttcccgtcgc gcaggatcgt
      661 gtgcgcgttc agcaggtccg gatcgttggt gacgttgttg ctgttgttct tcacctcgtt
      721 ccaggtcagg acgatctggc tatcttgggg ggcgagcgtg ccgttgccgg tggcggtcag
      781 gttctgcggc acgtcggggg tcgaggcctc cgccaccgtc gcgcatggcg ccgtcccggg
      841 cgcccagccg ctctggttgc ccacgacgtc ccgcgcctgc acctgcacgc aataggaggt
      901 cccgttgttc aggcccgcga cgaaaccgtg cccctcgaag tcgacgacgc cgaactcgct
      961 gtgcgggtac gtgacggctg cggtgctgaa cgggttcggc gcaccgttcg gccagtactt
     1021 gaggccgtac gaggtcactc ccgaggagct cgacggcgtg tcccacttga ccaggagccc
     1081 cttgcagtgg cccgggacga gggagatgtt ggtcggggcc ggcggcgggg tgacgtcgat
     1141 gtccttcacg ccggtcttcc cgaggttctc cgggttgacg tcggaggaca ggtcgaactt
     1201 gcggtagtgc gtcgtcgccg tcgcgtcgct cgcgtccacg tagtcgaggt cctcgtcggg
     1261 ggtcatgccg acgaggttca cgctgatctt ccggatgatg gagcgggtca gggccgtggc
     1321 gtcgccgccg ccaatgtcgt cggccgtgtt cgccggggtg ttcgcgttga gcggcaccgt
     1381 gccggcgtcc ggcatgtact ggaaggtcag ggtgcggatg ttgtccgcca ccggctcgta
     1441 ggagaagctg gtgaccgcct gcggtgcgcc ggggaacgcc ccggccacgt cccggagggt
     1501 cacccggtac agcgtgtagg ggggatcgtt ctggacgagc accacgttcg ggatggtgac
     1561 cgtctgcacc gccttgacgc gcggccggtt ggcgtccacc cggatggtga gggagtcgga
     1621 tccgaccggg cccggcttcg ccagcacgta agtgacgatc tcgtcgttgc cggtggagac
     1681 gacgttgtac accgcccccg gcaacgccgc ctccggcgag gcattggcgc tcgtgtcctc
     1741 gaagtcgaag tccccgcgga tggtgacggc ggtgtcccag gccccctcga tctgctcgtc
     1801 gacgcgggtg gcgtcgccgt ccgggttggt gttgaacccg gcgaggcgca tgtcggagat
     1861 catccggtcg aaggcgactc gggtcgactg ctgctgctcg gagaagttct cgcccgactt
     1921 gtacgacttc tgggccgccg tgtagaagat gaacaccacg gcggcgacga tggcgaagac
     1981 cgccgacgcg acgagcatct cgaccaggct gaatccgtgc tctctgttcc tcgggtcctg
     2041 tcgttgcgtc aacaccggag cctccgttcc gccgcggcga accgcggctt tgtggcgtcc
     2101 ttcagaatct caccgtcgac agggcgacgc tctgcggccg cccgagctcg ttccaggtca
     2161 ccctcaccgt caccttgatt ccggcggcgg ccccgaaatt gggcgtgccg ggccccatgg
     2221 cgtcgaggcg aaccgaggcg aagccgttgt tgagcttccg ggcgatctcg gtctgccacc
     2281 cgacgatcgg gcttcccgtg acgttgctca ggacggtgcg gttggaatcc gtcgcggccg
     2341 ccccgaggtt caggtagagc gaggcgaacg actccttgtc gaactgctcc atcaggtcct
     2401 gcgccagggt cgtcgcctcg gtcagggtct tgccggtctt cacctgccgc ccgcccatga
     2461 tgaacatcga ggcgatcgac agcatcaccc cggcgagcac gccgatggcg atgatcacct
     2521 cgatgaggct gaatcccgcg tccggcgatg tcctgtgatt ccgctcgatg gcgcgcatgc
     2581 tccggctcct cccctacggc gtgtagctgc tgaatgccgt gctgacggtg ccgctcggcg
     2641 acacgctgat ggtgtagcga tcgccgcgcg tgccgttgat gtccatgctg acgatgatga
     2701 cctcggcgcc ggccgacccg gtcgcgccgg tgatgctgaa ggacagggtg tcggcgctgg
     2761 cgtcctcgat gttgacgccc tgctccacgc gccggaggag cgtgtccccc acggcgttga
     2821 cgaaggtgta ggtgttcggg tcggtcccgg ccccctggga gttgatcgtc atcgtgcgcg
     2881 ggccgcgctg gccgacggcg gaatagcgca tggcgcggac gtcggtcgcc acgatgtccg
     2941 ccgccgcccg caccttgtag gcgcggaagg cgtccgccat ggccggcccg ccgaacagga
     3001 tgaagatccc catcagggcg atcaccacca gcagctcgga gagcgagaac ccttccgttc
     3061 tccgtttcat cgcttccttc cctcgctcgg tggtcatcgg ctctcctcag cgttcgcgcc
     3121 cccttcacgc aaggggggtg ccacggggga gcggggagat cgcggtgggc gcaagtccgt
     3181 gccccgcaag gggttgaggg gagggtcacg gcctcgtgat cgatgacgga atcaggatcg
     3241 cgggcacgag acgcggtgaa atgtaacgcc ggagcctgtc cgggccggtg tcgtcaggcg
     3301 acgagatcga tcgggaccgg cggcgcgggg cgcagcggga ggatcagcac catgatggtc
     3361 ccgccccctt cacgcggcag cgcctcgacc gtgccatcgt ggagcgccgc cgccttctgc
     3421 accagcggca gcccgagccc cgtcccctcc tgcttggtcg tgtagaacgg agtgaagatg
     3481 tgcccccggt ccgccggagg gatgccgggg ccgtcgtcct cgacttcgat gcggacgcgc
     3541 cccccctccc ccggctcctc gcggccgcgc acgacgatcg tccgcggggc gacgatccca
     3601 cccgcctccc gcaccgcctc cgaggcgttg cgcaggaggt tttgcagcgc ctgccgcagc
     3661 agcgtttcgt cggcgaaaag ggacgggaac gagccttcca ccgaaaccgt gaccagggcg
     3721 ttgccgggat cggcgcggag gtcgtccacc gcctcgcgca ccagcctcgc gagatcggtc
     3781 tccgcgagat cgggggtcgt ggggcgggcg tagcgcagga aatcgcccac caccttcagg
     3841 agccgcgcca cctcccgctc gatcgcctcg gcgtgccccc gggccggatt ctgcgcgagg
     3901 gcgcgcgact gcagtcgcgc gtgcccctgg atcaccgcga gggcattgcg gaactcgtgc
     3961 gcgatcccgg ccgacatctc gccgaggatc gccaggttgt ctcgcaggcg ggcccgctcc
     4021 ctgagggccc ggatctccgt caggtcggtg agcaggcaca cgaccccctc gatcgcggcg
     4081 tccccggttc ccgcgccggt cccccggatc ggagagagca tcacgccgac gtgcgccggg
     4141 cggccgtcgc ggccgcgcag cgtcaccacc tcgcgcgatc gcgcctcccc ccggccgagc
     4201 ccgccgcgca ccagatcgag gaggggcggg tgtgcggcga ggaggtccga ggcggagcga
     4261 cccggcggct gcggcccgac gctcaggagc ttctcggcgg ccgcgttgag ggtgaccaga
     4321 cgtccggcgc cgtcgaacac cagggccgcg ctcgtcatcc cggagaggag ccgctccccc
     4381 ggcaggcccg gcgccgaacc cgaggcccgc tcggcgctcc ccttgagcgc cagcagctcc
     4441 ccttcctggg cgcgcatctt ctcgagcacc ccctgcaccg ccgtcaccag cgcctcggcg
     4501 tcgtccatgc cggccgaggg atccgggacc gccgcgagcc cggcggcctg ctgcaggcgc
     4561 cggtacggcc gcaggagcca gcgcgcgaac agcagcacca gcagcaggac gaaggcgagc
     4621 ccgccggcct gcagggcggc gatgctgcgc aggtccagat cgaccgacgc gagcgccggg
     4681 acttcgcgct cgagccggag gatcgcctgg gtctcgtacg cgcggttgcg gatcggccgg
     4741 tacgcggcga gcgtcgcgaa cggcgatcgg cccgcgcggt cgagcggcgt caccgcggtc
     4801 gcaccggcga gcacgcggcg ccgctcccgc ccgccgtccc ggagcaggaa cggatcggac
     4861 cggccgatgc gggcgggatc cgacgacgcc acgacgcgac cgtcgatcgc gagcagatcg
     4921 acgcgccgca ggtcgtgcac ccgcaccagc cgctcgaggt gggccggcgg cacgaacgga
     4981 gtcgcctcgg tcggcgatgc cggtcgcagc atgtcgtcgc gctccagctc ggtgcgcagc
     5041 aggtccgcct cgacccccag gcgcagcccc gcctcctcct ggatggcgtt gcgcgcgatc
     5101 accaggaggt ggaagtcgaa atagattgcc accgccagga acagcaccag gaggaccagg
     5161 aagatcttga tctggccggc gtacgaccgc ccgaaggcgg gggtcacggg atcgcctcga
     5221 gacgcagggc gtaccagcgc acgatcggct ctccgacgaa c
//
LOCUS       DASRIQ010000183         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2945452, whole genome shotgun sequence.
ACCESSION   DASRIQ010000183 DASRIQ010000000
VERSION     DASRIQ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2945452"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VFD06_04625"
     CDS             <1..1624
                     /locus_tag="VFD06_04625"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HZN02851.1"
                     /translation="SLRYRGLDDVERVTRIAYSLPPISAGPSLLRFRIDLDPDTDRTL
                     SFTIDCNATEPASAPRPLPRKANPPDLWRGRVGASQAGFDSWIRRSYSDVAMMTTCTP
                     HGPFPYAGVPWFATPFGRDALLTAYEILWIAPGVARGVLRFLAAHQATKDDPERDAEP
                     GKIFHEMRDGEMPRLGEVPFDRYYGSVDSTPLFLMLAAAYFERTGDIDLMRELWPSFE
                     RAVAWMDGPGDRDGDGFLESQRRSEQGLENQGWRDSRDANMHADGTLAEGPLALCEVQ
                     AYAFAARAGLASIARALGRAGDADRLARGAEDLKQKFEKAFWLPSLGTYALALDGAKR
                     PCEVRSSCAGHALFAGIASPERAATVAQTLLSADHFSGWGVRTLARSAVRYNPMSYHN
                     GSVWPHDNALIGLGLARYGETAGAERILTALFEASRSIEASRLPELMCGFPRQPGEAP
                     TLYPVACSPQAWAAGAVFMLLQGLLGMSIHAETRQVRLSRPRLPPFLNALTIRQLRVG
                     EGELDLLLERADDEVVVRVLRRTAAVDLVLSS"
     gene            complement(1666..2259)
                     /locus_tag="VFD06_04630"
     CDS             complement(1666..2259)
                     /locus_tag="VFD06_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02852.1"
                     /translation="MNQEELTELIYQTLETEIGGVEVYATALECVQNEDLKEEFEKYH
                     EETKRHVEAVRGLCESLALDPGEETPGRLIVRHKGEALIAAMKEALKAGNPGAAEIVA
                     VECVVDAETKDHANWELIGEIVKKSKSESAGALKKAYDEIEEQEDEHLYHSKGWAREL
                     WIQALGMKPVLPPPEEEKDVHSAIEASRARHARERMM"
BASE COUNT          293 a          855 c          784 g          391 t
ORIGIN      
        1 cagcctgcgc taccgcgggc tggacgacgt cgagcgggtc acgcgcatcg cgtacagcct
       61 gccgccgatc tccgccggcc catcgctcct tcgcttccgc atcgatctgg atccggacac
      121 cgaccggacc ctgtcgttca ccatcgattg caacgccacc gagccggcga gcgcgccgcg
      181 ccccctgccg cgcaaggcga acccacccga cctgtggcgc gggagggtcg gggcgtcgca
      241 ggcgggattc gattcctgga tccgccgctc gtactccgac gtcgcgatga tgaccacctg
      301 cacgccgcac ggccccttcc cgtacgccgg cgtgccgtgg ttcgcaaccc cgttcggccg
      361 cgatgcactc ctcacggcgt acgagatcct ctggatcgcg ccgggcgtgg cgcggggcgt
      421 gctgcgcttc ctcgcggcgc accaggcgac gaaggacgat ccggagcggg acgcggagcc
      481 ggggaagatc ttccacgaga tgcgcgacgg cgagatgccg cgcctcgggg aggtgccgtt
      541 cgaccgctac tacggcagcg tcgactcgac gccgctcttc ctgatgctcg ccgccgccta
      601 cttcgagagg accggggaca tcgatctcat gagggagctg tggccgtcct tcgagcgcgc
      661 ggtcgcgtgg atggacggtc ccggagaccg ggacggcgac ggcttcctcg agtcccagcg
      721 ccggtcggag caggggctcg agaaccaggg atggcgggac tcgcgcgacg ccaacatgca
      781 cgccgacggg acgctggcgg agggaccgct ggcgctgtgc gaggtgcagg cgtacgcctt
      841 cgccgcccgt gccggcctgg cgtcgatcgc gcgcgccctc gggagggcgg gggacgcgga
      901 tcgcctggcg cgcggcgccg aggacctgaa gcagaagttc gagaaggcgt tctggctgcc
      961 gagcctcgga acctacgccc tggcgctcga cggcgcgaag cggccctgcg aggtgcgatc
     1021 gtcctgcgcc gggcacgccc tcttcgcggg catcgcgtcc cccgagcggg ccgcgaccgt
     1081 cgcccagacc ctgctgtcgg ccgatcactt ctccggctgg ggggtgcgga cgctggcgcg
     1141 ctcggcggtt cgctacaacc cgatgtcgta ccacaacggc tcggtctggc cgcacgacaa
     1201 cgccctcatc ggcctcggcc tggcgcgcta cggagaaacc gccggagcgg agcggatcct
     1261 caccgccctg ttcgaggcga gccgatcgat cgaggcctcc cgccttccgg agctgatgtg
     1321 cggctttccg cgccagccgg gtgaggcgcc gacgctctat ccggtcgcct gctccccgca
     1381 ggcgtgggcc gcaggcgccg tcttcatgct gctgcagggg ctgctcggca tgagcatcca
     1441 cgccgagacg cgccaggtcc gcctgagccg gccgcggctg cccccgttcc tgaacgccct
     1501 caccatccgg cagctgcgcg tcggggaggg ggaattggat ctcctccttg agcgggcgga
     1561 cgacgaggtg gtcgtgcggg ttctgcggcg cacggccgcc gtcgatctcg tcctctcgag
     1621 ttaacgaacg aacttccccc cctgaggcgc tgagttcgat ctcgcttaca tcatgcgttc
     1681 gcgggcgtgc ctggcgcggg aggcttcgat ggccgagtgg acgtcctttt cctcctccgg
     1741 cggcggcagc accggcttca tccccagcgc ctggatccac agctcgcgcg cccagccctt
     1801 cgagtggtag aggtgctcgt cctcctgctc ctcgatctcg tcgtaggcct tcttgagcgc
     1861 cccggcgctc tccgacttgc tcttcttgac gatctccccg atgagctccc agttggcgtg
     1921 gtccttcgtc tccgcgtcga cgacgcactc gacggcgacg atctccgcgg cgccggggtt
     1981 gcccgccttc agcgcctcct tcatcgcggc gatcagcgcc tcccccttgt ggcgcacgat
     2041 gagccggccc ggcgtctcct ctccgggatc gagggcgagg ctttcgcaga ggccgcgcac
     2101 cgcctcgacg tggcgtttcg tctcctcgtg gtacttctcg aactcctcct tcaggtcctc
     2161 gttctgcacg cactcgagcg ccgtcgcgta cacctcgacg ccgccgatct ccgtctccag
     2221 ggtctggtag atgagttcgg tcagctcctc ctggttcatg gtcgcggagc cgttccccgc
     2281 ggtggacgcg tggctcgacg atttggcccg ccgacccccc gac
//
LOCUS       DASRIQ010000184         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3194049, whole genome shotgun sequence.
ACCESSION   DASRIQ010000184 DASRIQ010000000
VERSION     DASRIQ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3194049"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /gene="rpsB"
                     /locus_tag="VFD06_04635"
     CDS             <1..793
                     /gene="rpsB"
                     /locus_tag="VFD06_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZN02853.1"
                     /translation="VQRIGSEGRTILFVGTKRQAQEVVAEEATRAGMPFVNTRWLGGT
                     LTNFRTIKKRIERLRWLENFLEKPVEGKYTKKEMLQLGKEQVKLAKVLSGIRHLDRLP
                     DGLFVIDPKKEHIAVLEARKLGIPVVGVVDTNCDPDDIDFPIPGNDDAIRAIKLFTGK
                     VADAVLQGRNAWEKRAADRNSDEGAMAAAGGGGVSGMPSLGEDPGRVAPRRAGAAGAA
                     RPAGPAKRKRENGDEAAPIVEVQATQGGEVPAPVGAAREGVATGE"
     gene            878..1504
                     /gene="tsf"
                     /locus_tag="VFD06_04640"
     CDS             878..1504
                     /gene="tsf"
                     /locus_tag="VFD06_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZN02854.1"
                     /translation="MEISASLVKELRDRSGAPMMECKTALKEAQGDLDGAHKILRQRG
                     QATAAKRSAKATSEGVVSAYLHGGGRIGVLIEVNCETDFVARTADFQNLVKDLAMHVA
                     ASDPRFIDRDEVTEAVLSEEREISRQQAKATGKPDAVVEKIVAGKMEKFYQEFCLLEQ
                     PFVRDPATSVKDVIHTVIAKTGENVRVRRFVRFVLGQGEVRVASARNS"
     gene            1575..>2176
                     /gene="pyrH"
                     /locus_tag="VFD06_04645"
     CDS             1575..>2176
                     /gene="pyrH"
                     /locus_tag="VFD06_04645"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZN02855.1"
                     /translation="MGTRPYGLDPAVVRRIAGEIAEVRGAGIEIAMVIGGGNIYRGLS
                     AAGEGLDRVTGDHMGMLATVINSLAMQDALEAVQVNTRVLSAIEIRQVAEPFIRRRAL
                     RHMEKGRIVIIAAGTGNPYFTTDTAAALRAMEMKADVLLKATQVDGVYSADPKKDAAA
                     ERYEEITYRQVIADGLRIMDASAIALCQENKVPIVVFHLGV"
BASE COUNT          380 a          712 c          801 g          283 t
ORIGIN      
        1 cgtgcagcgc atcggatcgg aaggacggac catcctgttc gtcggcacca agcggcaggc
       61 gcaggaagtg gtggccgagg aggcgacccg ggccgggatg cccttcgtca acacgcgctg
      121 gctgggcggc accttgacca acttccgcac catcaagaag cgcatcgagc ggctgcgttg
      181 gctggagaac ttcctggaga agccggtcga ggggaagtac accaagaagg agatgctgca
      241 gctcggcaag gagcaggtga agctggccaa ggtgctctcc ggcatccgcc atctggatcg
      301 cctgcccgac gggctgttcg tcatcgaccc caagaaagag cacatcgcgg tcctggaggc
      361 tcgcaagctg ggcatccctg tcgtgggggt cgtggacacg aattgcgatc ccgacgacat
      421 cgacttcccg atccccggca acgacgacgc catccgggcc atcaagctgt tcaccggcaa
      481 ggtggcggac gcggtcctgc aggggcgcaa cgcctgggag aaacgcgctg ccgatcgcaa
      541 ctccgacgag ggggcgatgg cggcggcggg cggcgggggg gtgtcgggga tgccgtcgct
      601 cggcgaagat ccgggacggg tggcgccacg gcgcgccggc gcagccgggg cggcccgacc
      661 ggccgggccg gcgaagcgca agcgcgagaa cggggatgag gccgctccga tcgtggaagt
      721 gcaggcaacg caggggggtg aggtcccggc cccggtcggc gccgcgcgcg aaggggtcgc
      781 gacgggcgag taactccgcc ccggcccggc ggaaggggcc ggggaggcct gccgcccgga
      841 catcacgcac acagaaatag aggagtcagg aggagtgatg gagatcagcg cgtctctggt
      901 caaggaactc cgcgatcgca gcggggctcc gatgatggag tgcaagacgg ccctgaagga
      961 ggcccagggg gacctcgacg gggcgcacaa gatcctgcga cagcgcgggc aggcgaccgc
     1021 cgccaagcgc tccgccaagg ccacctccga gggggtggtc agcgcctacc tgcacggcgg
     1081 cgggcggatt ggcgtcctca tcgaggtgaa ctgcgagacc gacttcgtgg cccgcaccgc
     1141 cgacttccag aatctcgtga aggacctggc catgcacgtg gccgcctcgg atccgcgctt
     1201 catcgaccgg gacgaggtga ccgaggcggt gctctccgaa gagcgcgaga tctcccgcca
     1261 gcaggcgaag gcgaccggga agccggacgc cgtcgtcgag aagatcgtcg ccggcaagat
     1321 ggagaagttc taccaggagt tctgccttct cgagcagccg ttcgtgcgcg accccgcgac
     1381 ctccgtcaag gacgtcatcc acacggtgat cgccaagacg ggtgagaacg tccgcgtccg
     1441 ccgcttcgtg cggttcgtcc tcgggcaggg tgaggtgcgc gtcgcctcgg cgcgcaactc
     1501 ctgacggcgg agggaccttg tcccggccgc cccgctaccg ccgcgtcgtc ctgaagcttt
     1561 cgggcgaggc cctcatgggg acccgtccct acgggctcga ccccgccgtc gtccggcgca
     1621 tcgccggcga gatcgccgag gtgcgcggcg ccgggatcga gatcgccatg gtgatcggcg
     1681 gcggcaacat ctaccgcggg ctgtcggcgg cgggcgaggg gctcgaccgg gtcaccggcg
     1741 accacatggg gatgctcgcc acggtgatca actccctggc gatgcaggac gcgctcgagg
     1801 cggtgcaggt gaacacgcgg gtgctgtcgg cgatcgagat ccgccaggtg gccgagccgt
     1861 tcatccgccg ccgcgccctg cggcacatgg agaaggggcg catcgtcatc atcgccgccg
     1921 gcaccggcaa cccctacttc acgaccgaca ccgccgccgc cctgcgggcg atggagatga
     1981 aggcggacgt cctgctcaag gcgacgcagg tggacggcgt ctacagcgcc gacccgaaga
     2041 aggacgccgc ggcggagcgc tacgaggaga tcacctaccg ccaggtgatc gcggacggcc
     2101 tgcgcatcat ggacgcctcc gccatcgcgc tgtgccagga gaacaaagtg ccgatcgtcg
     2161 tgttccatct gggcgt
//
LOCUS       DASRIQ010000185         4250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2946266, whole genome shotgun sequence.
ACCESSION   DASRIQ010000185 DASRIQ010000000
VERSION     DASRIQ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2946266"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFD06_04650"
     CDS             complement(<1..254)
                     /locus_tag="VFD06_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02856.1"
                     /translation="MSLTAQMHLMAGLGALGLGLGVFCAEPRRRRNRLFALLCGTLVF
                     WNLGVALERAGFAPSFPWHRLFLIGSCFAAPTGLHFFREV"
     gene            complement(906..2954)
                     /locus_tag="VFD06_04655"
     CDS             complement(906..2954)
                     /locus_tag="VFD06_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HZN02857.1"
                     /translation="MLQHDHRPNGDRAGCRRRASFLSALAILACAAPWTVAPADAAGL
                     LKPSDGGAPLAIEEHQVRVDINNGIAATEVVQVFRNDGPNPLEAVYSFPLPPESSLAG
                     FSMWINGQEMTGEVVERKRAREMYDSIVRPAVPDQQTAAWRPPVKDPGLVEEVSYREF
                     QVTIFPVPARGTQRVRLVYYEYLKAEDGRLTYLYPMETRATEDSRAHGRFSIEIGVRS
                     VVPIVESTFPGELDDQVRGETVDPHHLRATLTRADGDLDRDFIMSYVLNRDAPGLDLI
                     SYRAPGEAGYFMALATVPPTTPRAAPVDYTFIVDVSGSMRQRQRLAVAGEALARFLES
                     CPAEDRFQVIAFNVAPHVLSPDALANDRAGRDALIDFLAGFRGVGGTDLYPALEKAYT
                     QAAPGRRHAIVVVSDGGLNDLDEDHGRFLRLARGRPAVIFGLAIGNDANVPLLQALAT
                     ETGGFTAQVSGSDDLTRRGAALRERMRQEPLRDLHLTLDGDAELIDVVPARIPDLYPG
                     RQVAILGRYRGSGDVRWRLHGTSDGEPVVLEAAGALPQEDRLNPEIRRLWAERKAFDL
                     LQQTRAGRLPDAARDAVVRLGVDYSIVTPHTSFIVLESDRMFSDFGIERRNRALLEEE
                     SQARAQRARAIDARAVAAESDGPRSSPLSDLGGAVDPLLLAAILLLAAGPVIVRRLAP
                     "
     gene            complement(2984..4138)
                     /locus_tag="VFD06_04660"
     CDS             complement(2984..4138)
                     /locus_tag="VFD06_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02858.1"
                     /translation="MTWIAKTTFIAAGALAWSAPALAKVSVTQGLSVAGTPLLLTNQG
                     EVYSIAAAEAPARRLGRLPGHASSLVPSGQDAWAVVTGRQRAAAVRLYRIDAATGGIE
                     SIVPSHSSIDGPIEVDGLAGADSRFVYLLPDGRFDRDRQEILPGHLDAEPGLQLLRVL
                     VRGERAWYLARDRQPDPSGLVRLWLLHDSIWNDQGLRVMAGVENGPVDLYSDDRNLFL
                     VFEAGRVLRFDSASLRLLEDLSPMLRSEPIQFFAADGSHYWVGTRGAAEGGEVRRALW
                     RIERDNLDGAPLHAESLPEGYVPLVATEETLWFGSLERPDANPVVAVDKGDLTARTYG
                     VRGVHARRWRAFGRGALNTGERALIITAAVPVVAVAIVTAPLWIWWVPFC"
BASE COUNT          701 a         1453 c         1534 g          562 t
ORIGIN      
        1 cacacctcgc ggaagaagtg gaggccggtg ggagccgcga agcaggaacc gatcaagaag
       61 agacggtgcc agggaaagga aggggcgaag ccggcgcgtt ccagcgcgac gccgaggttc
      121 cagaacacga gcgtgccgca cagaagcgcg aacaaccggt tgcgccgccg ccgcggctcg
      181 gcacagaaga ccccgaggcc caggcccagc gctccaagcc ccgccatgag gtgcatctgc
      241 gccgtgagcg acaccggact cctgtcgtga cgacgcgatt ctatccgagc ctccgcgtgc
      301 ggcgcgcgcc cgcggcagcc gccgtcgcca gagcgccgac aggcacgcga ccagggctcc
      361 gcaggcagct ccgaggaggt gcgccgccgg cgacggaacc gccccagcca gggcggcgga
      421 ccccgcgaag accggcgcgc cccggtgcgt ctccgtcacc agcttggcga ggaagaggcc
      481 ggcggcgagc gagggcacca gcgcctccca tgggcggcgc gagcgcatcg actgcatcag
      541 tcgcgcgact agaagagccg cgaactgggc gctggcgatc cccgacagcc cgcggtagct
      601 gccgagctga gggtgcaaga ggagcaggcc ggcccccacg ccgacggcag cggcgacgag
      661 cagtgccagg taatgccccc gcagctccgg ctcgcacagg cttccgagaa tcagaaaggc
      721 gaggacgttg agggcgagat gggccggagt caggtgggcg agatgggagg tcacaatccg
      781 ccagggctcc accatcggca cctgaagccc gcgcagctcg agccgggccg ggaggccgtc
      841 ccatgcgccc actgccatgg ccagcagggc gacgccgatc gtccagcgtc gcgagcggac
      901 ccagctcatg gcgcaagccg ccgcacgatg accgggcctg cggccagcag gagaatcgcc
      961 gcaagcagga gaggatcgac ggcgccgccg agatccgaca gcggactgga acggggtccg
     1021 tcggactcgg cggcaacggc gcgcgcgtcg atcgcccgtg cgcgctgggc ccgcgcctgc
     1081 gactcctcct cgaggagcgc ccggttgcgg cgctcgatgc cgaagtcgct gaacatgcga
     1141 tcgctctcga gcacgatgaa cgaggtgtgc ggcgtcacga tcgagtagtc aacgccgaga
     1201 cgaacgacgg cgtcgcgcgc ggcgtcgggc agccggccgg cgcgcgtctg ctggaggagg
     1261 tcgaaggcct tgcgctccgc ccagagacgg cggatttccg ggttcaggcg atcctcctga
     1321 ggcagcgccc cggcggcttc cagaacgacc ggctctccgt ccgatgttcc gtgaagcctc
     1381 caccggacat ctcccgaccc gcggtaacga ccgagaatcg ccacctgacg gccgggatag
     1441 agatcgggga tacgggccgg gacgacgtcg atcagctcgg cgtcgccgtc gagcgtcaga
     1501 tgcaggtcgc gcaggggctc ctggcgcatg cgctcgcgga gcgcggcacc gcgccgcgtc
     1561 aggtcgtcgc tgccggagac ctgagccgtg aacccgcccg tctccgtggc gagcgcctgc
     1621 agcagcggga cgttggcgtc gttgccgatc gccaggccga agatgacggc cggccggccg
     1681 cgcgcgagcc gcaggaagcg gccgtgatcc tcgtcgaggt cgttcagccc tccatcgctg
     1741 acgacgacga tcgcgtgacg ccgccccgga gcggcctgtg tgtaggcctt ctcgagcgcc
     1801 gggtagaggt cggttccgcc gacgccccgg aacccggcga gaaagtcgat caacgcgtcg
     1861 cgtcccgcgc ggtcgttcgc aagcgcatcc ggcgagagga cgtggggagc gacgttgaag
     1921 gcgatgacct ggaagcggtc ctccgccggg cacgactcca ggaaccgcgc cagcgcctcg
     1981 ccggccacgg cgagccgctg tctctgacgc atcgatccgg acacgtcgac gatgaaggtg
     2041 tagtcgacgg gcgcggccct gggcgtcgtc gggggaaccg tggcgagcgc catgaaatac
     2101 ccggcttcgc ccggcgcgcg gtagctgatc aggtccaggc ccggcgcgtc ccggttgagg
     2161 acatagctca tgatgaagtc gcggtcgaga tcgccgtcgg cgcgcgtcag ggtggcgcgc
     2221 agatggtgcg gatcgacggt ctcccccctc acctgatcgt ccagctcgcc cgggaaggtg
     2281 gattcgacga tcggcacgac cgaacgcacg ccgatctcga tcgagaagcg gccgtgggcc
     2341 cgcgagtcct cggtggcgcg cgtctccatc gggtacagat acgtgaggcg gccgtcttcg
     2401 gctttgaggt attcgtagta gacgagccgc acgcgctgcg tgccgcgggc agggacgggg
     2461 aagatggtca cctggaactc gcgatacgag acctcttcga ccagccccgg atccttgacg
     2521 ggtggcctcc aggccgccgt ctgctgatcg gggaccgcgg ggcgcacgat gctgtcgtac
     2581 atctcccggg cccgtttgcg ttccaccact tcgccggtca tctcctggcc gttgatccac
     2641 atggagaagc cggccaggct cgattcgggt ggcaggggaa acgaatagac cgcttcgagc
     2701 gggttcggac cgtcattgcg gaagacctgc acgacctcgg tcgcggcgat gccgttgttg
     2761 atgtcgacgc ggacctggtg ttcctcgatg gcgagcggcg cgccgccgtc ggagggcttg
     2821 agcagccccg ccgcatcggc gggcgcgacc gtccacggcg ctgcgcatgc caggatggcc
     2881 agcgcggaga ggaacgacgc ccggcgacgg catcccgcgc ggtctccgtt cggacgatga
     2941 tcgtgttgaa gcatggtgtg tcctcctcgt cgatgcgcgg cggtcaacag aatggaaccc
     3001 accagatcca gagcggcgcg gtcacgatcg ccacggcgac aacggggacc gcggccgtga
     3061 tgatcaaggc cctctcgcct gtgttcaggg cgccgcggcc gaacgcccgc cagcgacgtg
     3121 cgtggacccc tcgcacgccg tacgtgcgcg cggtcaggtc tcctttgtcg accgccacca
     3181 cggggttcgc gtccggcctt tcgagcgagc cgaaccagag ggtctcctcc gtcgcgacga
     3241 gagggacgta cccctcgggc agcgattcgg cgtgaagcgg ggcgccatcg aggttgtcgc
     3301 gctcgatgcg ccacagtgcc cgacgcacct cgccgccctc ggcagcgcca cgggtaccga
     3361 cccagtagtg gctgccgtcc gcggcgaaaa actggatcgg ctccgaacgc agcatcgggc
     3421 tcagatcctc gagcaggcgc agcgaggcgc tgtcgaaccg cagcacgcgt cccgcctcga
     3481 agaccaggaa caggttccgg tcgtccgagt agagatcgac cggaccgttc tcgaccccgg
     3541 ccatgacgcg cagaccctgg tcgttccaga tcgaatcgtg caggagccag agacggacca
     3601 gtccggaggg gtccggctgg cgatcgcgcg ccaggtacca ggccctttct ccgcggacga
     3661 ggacgcgcag cagctgcagg ccgggctcgg cgtcgaggtg cccgggcagg atctcctggc
     3721 ggtcgcggtc gaaacgccca tccggcagga gatagacgaa acgcgagtcg gcgccggcga
     3781 gcccgtcgac ctcgatcggc ccgtcgatcg aggagtgcga ggggacgatc gactcgattc
     3841 cccctgtcgc tgcgtcgatg cgatacaggc ggacggcggc ggcgcgctgc cgcccggtca
     3901 ccaccgccca cgcatcctga ccggagggca ccagagacga ggcgtgcccg ggcagccggc
     3961 cgagacgccg ggccggcgcc tccgcggcgg cgatggaata gacctctccc tggttcgtca
     4021 gcaggagcgg ggtgccggcc accgagagtc cctgggtgac gctgactttc gcgagggccg
     4081 gcgccgacca ggcgagagca cccgctgcga tgaaggtcgt cttcgcgatc catgtcatgg
     4141 tcgtctcctc cgcgcgcgtc cgtcctgcag acggcgtgcc gctggtctga ctctggaaag
     4201 tcgctctcga tccgagggat ttgcaggccg cgtgggtgac gcgcgcgcag
//
LOCUS       DASRIQ010000186         3616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1133090, whole genome shotgun sequence.
ACCESSION   DASRIQ010000186 DASRIQ010000000
VERSION     DASRIQ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133090"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VFD06_04665"
     CDS             complement(<1..159)
                     /locus_tag="VFD06_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN02859.1"
                     /translation="MSERFKGKVAVVTGGNSGIGLATAKAYVREGAKVAITGRSDATL
                     KSAQKELGP"
     gene            complement(380..2650)
                     /locus_tag="VFD06_04670"
     CDS             complement(380..2650)
                     /locus_tag="VFD06_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN02860.1"
                     /translation="METRSGARRAHVRSGFVVSSVVLSLCVGVPSSRGADPSTPERGS
                     IAGKVVESGGGPAPEAKVTIFELRRSVQAGADGSFRFDDVPPGTYLLEVVSPKFGSAV
                     VSAPVAAGQTAEVSVRLDLTVHHEDIVVSAGPLVQSASSVAAPVSVLDGPDLQAAVQP
                     TLGETLAREPGVSATSFSPGVSRPVIRGQGGDRIRILEDGIGVGDASNVSEDHAVAFD
                     PLAAERIEVVRGPATLMYGSNASGGVVNVFDGRIPAEANPEAIAGAFEARFGSNSDEA
                     AGLLNLNGGVKQFGWHLDALKRDTDDFEAGDGVPLVNSDLETRQGTAGASWTGKDGFL
                     GASWGRFETNYGIPEPDEPVRIDMHQDRVDLRGEYTAPLGIFQGIKVRAGRVDYEHAE
                     LEDTGEVGATFFNNAWEGRVEATHKQAGRFRGAFGVQYYSRDFEVVGEEAFVPPTVTR
                     NGAFFAFEQIGAGKNLTFEVGARYETQDNTVEDPVAPDRSFNGVSGSGAVVWKMPRDY
                     ALAFTVSRSERLPAAEELYANGPHLATFEFQIGDPNLSSETGLSYDLTFRRTEGIVTG
                     SISVFATRYSRYIFLSPNGLCVDADGAPLPCTDPDAIPEFNYVQTDADFRGAEAHVDI
                     ELYHTDPRHVTLEIGADTVRAEEQDSGDDLPRITPTRYSLGVRYRGPRLWGLVEGRRT
                     EEQDRTAPLETPTDGYTFLNVVLGYRILAAGLVHDIILRGTNLTDELARNHISPLKDI
                     VPLPGRDIGLSYRLTF"
     gene            complement(2774..3118)
                     /locus_tag="VFD06_04675"
     CDS             complement(2774..3118)
                     /locus_tag="VFD06_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02861.1"
                     /translation="MSSTSSGPLRSQLVGATLAAVVAIVAVAGAWHEHAFLLEGFHLC
                     APTEAAPKHHDHDCLACKIAPPFATPWNASTLVDPGLAGRQTFVPHEAQALSTAPIDT
                     AAPRGPPLLPQA"
     gene            complement(3164..>3616)
                     /locus_tag="VFD06_04680"
     CDS             complement(3164..>3616)
                     /locus_tag="VFD06_04680"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HZN02862.1"
                     /translation="TLVMSGEAKIAWQGERPFLSTGTNDYGAQLALQRKFSRQAVYCN
                     ASLVSTDGRVFGLELGRRVIPTFTAAWEVEMTEFTNLIGQVYASKSTIRDTSMEVITA
                     DKYQVSVGVRSRRGGLIYGFAFTENVKNFDNTPDVGLTLTLAWADLMP"
BASE COUNT          522 a         1230 c         1286 g          578 t
ORIGIN      
        1 cggccccagc tccttctgtg ccgacttcag ggtggcgtcg ctgcggccgg tgatcgccac
       61 cttcgccccc tcgcgaacgt acgccttggc cgtcgcgagg ccgattccgc tgttgccgcc
      121 cgtcacgacc gccaccttgc ctttgaatct ctcgctcatc gcaacctcct cttgtgcgtt
      181 ggacgtggat gcagcacttc aatcttcatg ccacgggcgc gcggcggccc ggcctgcggc
      241 gaacgcattg agcggccggc gctttcgtcg tggtctggtc ctccggatgc tcccacgaca
      301 ttgcgcggaa ttcgacacgt gcccgaatag cttcagcgag aaggtccccc aatggacatc
      361 agggggcggc ctcctcccct cagaacgtca gccggtagga gaggccgatg tcgcgtcccg
      421 gcagcggcac gatgtccttg agcggggaga tgtgattgcg cgccagctcg tcggtgaggt
      481 tcgtgccgcg caggatgatg tcgtgcacga ggcccgcggc gaggatccgg tagccgagga
      541 cgacgttgag gaaggtgtag ccgtcggtcg gagtctcgag cggggcggtg cggtcctgct
      601 cttcggtccg gcgtccttcg accaggcccc agagacgcgg gccgcgatag cggaccccga
      661 ggctgtagcg ggtcggggtg atgcgcggca ggtcgtcgcc cgagtcctgc tcttcggccc
      721 gcaccgtgtc ggcgccgatt tcgagggtca cgtggcgcgg atccgtgtgg tacagctcga
      781 tgtccacgtg cgcctcggcg ccgcggaaat cggcgtccgt ctgaacgtag ttgaactccg
      841 ggatggcgtc cgggtcggtg cacgggagcg gcgcgccgtc cgcgtccacg cagaggccgt
      901 tcggcgacag aaagatgtaa cggctgtatc tggtggcgaa gacggaaatc gacccggtga
      961 cgattccctc ggtgcgccgg aaggtgagat cgtaggagag gccggtctcc gacgagaggt
     1021 tcgggtcgcc gatctggaat tcgaacgtgg cgaggtgcgg gccgttggcg tacagctcct
     1081 cggcggccgg caggcgctcg gagcgggaca cggtgaaggc cagcgcgtag tcgcgcggca
     1141 tcttccagac gacggcgccg gagccggaca cgccgttgaa ggagcggtcg ggagcgaccg
     1201 ggtcctccac ggtgttgtcc tgcgtctcgt agcgcgcgcc gacctcgaag gtcaggttct
     1261 tccccgcgcc gatctgctcg aacgcgaaga aagcgccgtt gcgggtcacc gtcggcggca
     1321 cgaacgcttc ctccccgacg acctcgaagt cgcgggagta gtactgcacg ccgaaggccc
     1381 ctcggaagcg gcccgcctgc ttgtgggtcg cctcgacgcg cccctcccac gcgttattga
     1441 agaaggtggc tccgacctcc cccgtgtcct cgagctccgc gtgctcgtag tccacccggc
     1501 cggcgcgcac cttgatcccc tggaagatgc ccagaggcgc cgtgtactcg ccgcgcaggt
     1561 cgacacggtc ctggtgcatg tcgatgcgca ccggctcgtc cggctcgggg atgccgtagt
     1621 tggtctcgaa ccggccccac gaggcgccga ggaagccgtc cttgccggtc caggacgccc
     1681 cggcggtgcc ctgcctggtt tcgaggtcgc tgttgacgag gggcaccccg tcgcccgcct
     1741 cgaagtcgtc ggtgtcgcgc ttcagcgcgt cgaggtgcca gccgaactgc ttcaccccgc
     1801 cgttgaggtt gaggagaccg gccgcctcgt cggaattcga tccgaagcgc gcttcgaacg
     1861 ccccggcgat cgcctccggg ttcgcctcgg ccgggatgcg cccgtcgaag acgttcacca
     1921 cgcccccgct ggcgttgctg ccgtacatga gcgtcgcggg gccgcgcacc acctcgatcc
     1981 gctcggccgc gagggggtcg aacgcgaccg cgtgatcctc gctgacgttg ctggcgtcgc
     2041 cgacgccgat gccgtcctcg aggatgcgga tccggtcccc tccctggccg cggatcaccg
     2101 gacggctcac tccggggctg aatgaggtcg cgctcacccc cggctcgcgc gccagcgtct
     2161 cgccgagggt cggctgcacc gccgcctgca ggtcgggccc gtcgaggacc gacaccggcg
     2221 ccgcgacgga ggaggcgctc tgcaccagcg ggccggcgct gaccacgatg tcctcgtggt
     2281 gcaccgtcag gtcgagccgc accgaaacct cggcggtctg gccggcggcg acaggggcgc
     2341 tgaccacggc gctgccgaac ttcgggctga ccacctcgag caggtaggtc ccgggcggaa
     2401 cgtcgtcgaa gcggaaggag ccgtccgctc cggcctggac gctgcggcgc agctcgaaga
     2461 tggtcacctt cgcctcggga gcgggccccc cgccgctctc gaccaccttc ccggcgatcg
     2521 agcctctctc cggtgtggag gggtcagccc ctctggagga cggaacgccc acgcacagcg
     2581 acagcacgac cgacgacacg acgaagccgg aacgaacatg ggcgcggcgc gcccccgaac
     2641 gagtctccat gacgatctcc ccagtgagac aacggccatg ggaaagcagc gcgtgtgcgg
     2701 acgccggccg ccgcttcagg cggcgcgggc gccgtcaccg gcgcgcggat ctttcccgtt
     2761 ctctgtggtg gaactaggcc tgggggagga ggggcgggcc gcgcggcgcg gccgtgtcga
     2821 tgggggctgt cgaaagagcc tgcgcctcgt gcgggacgaa cgtctggcgg ccggcgaggc
     2881 cgggatcgac gagcgtcgag gcgttccacg gcgtcgcgaa cggcggggcg atcttgcagg
     2941 cgagacagtc gtggtcgtgg tgtttcggcg ccgcctcggt gggggcgcag aggtgaaagc
     3001 cttcgagcag gaacgcgtgc tcgtgccagg cgccggcgac cgcgacgatg gcgacgaccg
     3061 ccgccagtgt cgcgccgacc agctgcgatc ggagtgggcc tgacgatgtc gaactcatcc
     3121 tcatcgaacc ttggcgcgcc gggacgagcc gcgaacggag agtctacggc atcaggtcgg
     3181 cccaggcgag ggtcagcgtc aacccgacgt cgggggtatt gtcgaagttc ttgacgttct
     3241 cggtgaaggc gaagccgtag atcagcccgc cgcgacggct gcgcacgccg acgctgacct
     3301 ggtacttgtc ggcggtgatc acctccatgg aggtgtcccg gatggtgctc ttgctcgcgt
     3361 agacctgccc gatcaggttc gtgaactccg tcatctccac ctcccaggcc gcggtgaagg
     3421 tcgggatgac gcggcgcccg agctcgaggc cgaacacccg gccgtcggtg ctcacgagac
     3481 tggcgttgca gtagaccgcc tgccggctga acttccgctg cagagcgagc tgcgcgccgt
     3541 agtcgttggt ccccgtcgag aggaacggcc gctccccctg ccaggcgatc ttcgcctcgc
     3601 cgctcatgac gagcgt
//
LOCUS       DASRIQ010000187         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2694962, whole genome shotgun sequence.
ACCESSION   DASRIQ010000187 DASRIQ010000000
VERSION     DASRIQ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694962"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFD06_04685"
     CDS             <1..875
                     /locus_tag="VFD06_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN02863.1"
                     /translation="VSILDDFSTGHRRNLDAALKARPADAPAPEILEGDLRDVALVRR
                     AVAGVTHVLHQAALPSVPRSVAEPLMSHEVNASGTLGLLLAARDAGVKRFVYASSSSA
                     YGDAPTLPKIESQPTAPLSPYAVSKLAGEQYCRIFTPLYGLETVSLRYFNIFGPRQDP
                     QSQYAAVIPRFVTAVQRGESPSVYGDGTQSRDFTYIDNAVQANLLACDAPASAAGLAY
                     NVACGASATLLEVLGLIGRILNKPVRPVHEPFRAGDVKHSLASIDLARRHLDFDPKID
                     LEEGLRRTAAAFAG"
     gene            872..1552
                     /locus_tag="VFD06_04690"
     CDS             872..1552
                     /locus_tag="VFD06_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN02864.1"
                     /translation="MKLSIVIPVYNERETLPKTLARVQATPFDKEIILVDDGSTDGTR
                     TWLETLRDPGVVVVLQPENRGKGAALRAGFERATGDVVLIQDADLEYDPVDYPRLLQP
                     ILDDQADVVFGSRFGGESHRVLFFWHFVGNRFLTTLSNMFTNLNLSDMETGYKVFRAG
                     VLKRLTLRSNRFGFEPEVTAKVARLGCRIYEVAISYHGRSYEEGKKITWKDGFAAIGC
                     ILRYRFFD"
     gene            1518..2618
                     /locus_tag="VFD06_04695"
     CDS             1518..2618
                     /locus_tag="VFD06_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZN02865.1"
                     /translation="MGASSVTASSTEGRRPPVYAVILAGGRGERLWPRARRGRPKPFV
                     PLLGRGTLFEATARRARALAGSERVLVVCGADQARRVRSLSPWIDPDRIVREGVGRNT
                     AAAVALAALWVERRAGDAVVVILPADHSVSPPGRFLATVRRAARAVARREGLAVLGVP
                     ATKPERGYGYIGLGRPAGLRGVRLAAGFREKPSAALAARMVRGGRHLWNCGIFVGRAS
                     ILLRELQRLAPRVLGPLRHFATGARGPWTVPSHVLRAIPALPFDRAVLERTRDLMVVP
                     AGYRWSDLGTWSGLADAMQDRGAPVGEGTVVQSGSKRCAAFNPGGLTAFVGVRDLVVV
                     RDGDVVLVCRRDASQGVRKVVAGLRGSLSRHA"
     gene            2611..>3293
                     /locus_tag="VFD06_04700"
     CDS             2611..>3293
                     /locus_tag="VFD06_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02866.1"
                     /translation="MRETTSGEETAIRWCDAVIRWGLVALIAFTPLAFGTVEAWSIAV
                     MEWGVASLLLVAVARRILVGPAPEGTRWTTGVEWPLGLFLGLVLLQLVPLPRPLVAVL
                     APGSASAHASALAAPENGVTTRVDFEALRAALSLGPPPDRVPVSLDREETAHRTGLLL
                     TFASVFYLVAGWGATKGRAQFLLRAIVIVGFCVALFSIVQRLTWNGKIYWLRPSPQAT
                     ATGPFVNHN"
BASE COUNT          421 a         1179 c         1172 g          521 t
ORIGIN      
        1 cggtgtcgat cctcgatgac ttctccaccg gccaccgcag gaacctcgac gcggccctga
       61 aggcgcggcc cgcggatgcc cccgcccccg agattctcga aggggacctg cgcgacgtgg
      121 cgctggtccg ccgggccgtc gccggcgtca cccacgtgct gcaccaggcg gcgctccctt
      181 cggtgccgcg ctcggtcgcg gagccgctca tgagccacga ggtgaacgcc agcggcacgc
      241 tcgggctgct cctcgccgcc cgcgacgccg gggtgaaacg cttcgtctac gcctcctcct
      301 cgtcggcgta cggcgacgcc ccgacgcttc caaagatcga atcgcagccg acggcgccgc
      361 tgtcccccta cgccgtgagc aagctggcgg gggagcagta ctgccgcatc ttcacgccgc
      421 tgtacggcct cgagacggtg tcgctgcgct acttcaacat cttcggaccg cggcaggacc
      481 cgcagtcgca gtacgccgcg gtcatcccgc ggttcgtgac cgcggtgcag cgcggcgagt
      541 ccccgagcgt gtacggcgac gggacgcagt cgcgcgactt cacgtacatc gacaacgcgg
      601 tgcaggcgaa tctcctggcg tgcgatgctc ccgcgtccgc cgccggcctc gcctacaacg
      661 tcgcctgcgg agcgagcgcc accctcctcg aggtgctcgg cctcatcgga cggatcctga
      721 acaagccggt ccgcccggtg cacgagccgt tccgcgccgg cgacgtgaag cactcgctcg
      781 ccagcatcga cctggcgcgc cggcacctgg atttcgatcc gaagatcgat ctcgaggaag
      841 gactgcgacg aacggcggcc gcgttcgccg gatgaagctc tccatcgtca tccccgtcta
      901 caacgaacgc gagacgctgc cgaagaccct cgcgcgggtg caggctacgc ccttcgacaa
      961 ggagatcatc ctcgtcgacg acggatcgac cgacggcacg cgcacctggc tcgaaacgtt
     1021 gcgcgacccg ggcgtggtgg tggtgcttca gccggagaac cgcggcaagg gggcggccct
     1081 gcgggccgga ttcgagcgcg ccaccgggga tgtcgtgctg atccaggacg ccgacctgga
     1141 atacgacccg gtcgactacc cgcgcctgct gcagccgatc ctcgacgacc aggccgacgt
     1201 cgtcttcggg tcgaggttcg gcggagagag ccaccgcgtg ctgttcttct ggcacttcgt
     1261 gggcaaccgc ttcctgacga cgctctccaa catgttcacc aacctcaacc tctccgacat
     1321 ggagaccgga tacaaggtct tccgcgccgg ggtgctgaag cggctgacgc tccgctccaa
     1381 ccgcttcggg ttcgagccgg aggtcaccgc caaggtggcc cgcctcggct gccgcatcta
     1441 cgaggtcgcg atttcgtatc atggccgcag ctacgaggaa gggaagaaga tcacgtggaa
     1501 ggacggcttt gccgcgattg ggtgcatcct ccgttaccgc ttcttcgact gaagggcgcc
     1561 gtccccctgt ctacgcggtc atcctcgcgg gcggcagggg ggagcgtctg tggccgcgcg
     1621 cgcgccgggg ccggccgaag ccgttcgtcc cgctcctcgg ccgcggcacc ctgttcgagg
     1681 ccacggcacg gcgggcccgg gccctggccg ggagcgagcg ggtcctcgtg gtgtgcggcg
     1741 cggatcaggc ccgccgggtg cgatcgctgt cgccgtggat cgatccggat cggatcgtgc
     1801 gcgagggggt cggccgcaac acggccgccg cggtggccct ggcggcgctc tgggtggagc
     1861 gcagggcggg ggacgcggtc gtcgtgatcc tgcccgcgga tcattccgtt tccccgccgg
     1921 gacgctttct cgcgaccgtg cggcgggcgg cgcgggcggt cgcgcgccgg gagggactcg
     1981 ccgtcctggg cgtccccgcg acgaagccgg agcgcgggta cgggtacatc ggcctcggtc
     2041 gccctgcggg tctccgcggg gtccggctgg cggcgggctt ccgggagaag ccctcggcgg
     2101 cgctggcggc ccggatggtg cgcggcggcc ggcatctctg gaactgcggg atcttcgtcg
     2161 gacgggcctc gatcctgctc cgggagctgc agcgcctggc gccgcgcgtg ctcgggccgc
     2221 tgcgccactt cgcgacgggc gcgcggggcc cctggaccgt gccgagccac gtcctccggg
     2281 cgatccccgc gcttccgttc gatcgggcgg tcctggaacg aacgcgcgac ctgatggtcg
     2341 ttccggccgg ctaccgatgg tcggacctgg gaacctggtc ggggctcgcc gacgccatgc
     2401 aggaccgcgg cgcgccggtg ggagagggga ccgtcgttca gagcggctcg aagcgctgcg
     2461 cggcgttcaa tccgggcggc ctgacggcgt tcgtcggcgt gcgggatctg gtggtggtcc
     2521 gggacggcga tgtcgtcctc gtctgccggc gcgacgcctc ccagggggtc cgcaaggtcg
     2581 tcgcgggact gcgcgggagt ctttcgcggc atgcgtgaga ccacctccgg cgaggagact
     2641 gcgatccggt ggtgcgacgc cgtgatccgt tgggggctgg tcgcgctcat cgcgttcact
     2701 cccctggcgt tcggcaccgt ggaggcatgg tcgatcgccg tcatggaatg gggggtggcg
     2761 agcctcctcc tggtggcggt ggcgcggcgg atcctcgtcg gccccgcccc ggaaggaacc
     2821 cgctggacca ccggcgtcga gtggccgctc ggcctcttcc tcggactcgt gctgctgcag
     2881 ctcgttcccc tcccgcgccc tctggtcgcg gtcctcgccc cgggctcggc ctccgcccac
     2941 gcgtcggcgc tcgcggcccc cgagaacggc gtcacgacgc gggtggactt cgaagccctc
     3001 cgggcggcct tgagcctcgg tcctcctccg gaccgggtgc cggtgagcct cgatcgcgaa
     3061 gagacggcgc accggaccgg gctgctcctg accttcgcga gcgtcttcta cctggtggcc
     3121 ggatgggggg cgacgaaggg acgcgcccag ttcctgctgc gagccatcgt catcgtcggc
     3181 ttctgcgtcg cgctgttctc catcgtccag cgcctgacct ggaacggcaa gatctactgg
     3241 ctgcggccgt ccccccaagc gaccgccacc ggtccgttcg tcaatcacaa tca
//
LOCUS       DASRIQ010000188         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1641452, whole genome shotgun sequence.
ACCESSION   DASRIQ010000188 DASRIQ010000000
VERSION     DASRIQ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641452"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFD06_04705"
     CDS             complement(<1..311)
                     /locus_tag="VFD06_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02867.1"
                     /translation="MSLDRLARLGFVLVLSLAAASPAGAATSFDFLFSMDRVHNDNQY
                     FLNLAVSNFGYQRPALEPLLPRVRYVEADLPVVFFLQHASGWPLPNLVDLRAQGASWS
                     V"
     gene            444..1349
                     /locus_tag="VFD06_04710"
     CDS             444..1349
                     /locus_tag="VFD06_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZN02868.1"
                     /translation="MPELPDVTVYIECLERRILGRPLLKVRLASPFLLRSVTPPLSEA
                     EGTKVIGLRRLGKRIVLALEEDLFLVLHLMIAGRLRWNDAGAKVPGRLGLAALDFPDG
                     TLLLTEAGSKKRASLHLVRGEEGLEPFLCRGLEVLDSTPEEFASALRSENHTLKRALT
                     DPRLFSGIGNAYSDEILHRARLSPMQLTSRLGDEEAARLHAATVATLRDWTERLRRET
                     GEGFPEKVTAFREGMAVHGRFGQPCPDCGTSVQRIVYVENECNYCPRCQTEGRLLADR
                     ALSRLLKDDWPKTIEEMEGVSGGRG"
     gene            complement(1366..>2305)
                     /locus_tag="VFD06_04715"
     CDS             complement(1366..>2305)
                     /locus_tag="VFD06_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN02869.1"
                     /translation="AVDEVLLIAREAGLPAEIYHLKAAGEENWPKLDAVIAKVEAARA
                     EGLPITADMYTYTAAATGFDACLPPWAREGDQEALYRRLRDPKSRAMIYKEMASAAEG
                     WENICNLARSPASMLLLEFKNPALKPLQGKRLAEVAWERKKDWPEVVMDLILEDRTRV
                     GVAFFLMSEDNVRRQIQLPWVSFGSDAASMSPEGVFLKSSAHPRAYGNFARLLGHYVR
                     EEKLISLEEAVRRLTGLPATNLGLDRRGFLKEGQFADVAVFDPATIVDRATFDKPHQY
                     AEGVHHVLVNGVPVLKDGRHTDALPGRALKGPGATR"
BASE COUNT          358 a          768 c          815 g          364 t
ORIGIN      
        1 atgaccgacc agctcgcccc ctgggcgcgc aggtcgacga ggttcggcag cggccatccc
       61 gaggcgtgct gcaggaagaa caccaccggg aggtcggcct cgacgtagcg cacccgcggc
      121 aggagcggct cgagggccgg ccgctggtag ccgaaattgc tgacggcgag attgaggaag
      181 tactggttgt cgttgtggac ccggtccatg ctgaacagga agtcgaacga cgtggccgca
      241 ccggccggag aagccgccgc gagcgagagg acgaggacga agccgagccg ggccaggcga
      301 tccaaggaca tgatgatctc ctcctgtcga ggtcgggcgt tggcgttacg aggtgagatt
      361 ctaggtccgg ccggggtatt ccgccagcgg accgtgaggg tcgggatcgt agaatcggag
      421 accttcgagg agggctgcag cgcgtgccgg agcttcccga cgtcacggtc tacatcgagt
      481 gcctcgagcg ccggatcctc gggcggccgc tcctgaaggt ccgcctggcg agccctttcc
      541 tcctgaggag cgtgacgccg ccgctctcgg aggcggaagg gacgaaggtc attgggctgc
      601 gccgcctcgg caagcggatc gtcctggcgc tcgaagaaga tctcttcctc gtcctgcacc
      661 tcatgatcgc cggccggctg cgctggaatg acgcgggcgc caaggtgccg ggacggctcg
      721 gcctcgcggc gctcgatttc cccgacggca ccctcctcct cacggaggcc ggctcgaaaa
      781 agcgcgcctc cctccacctg gtgcgcggcg aggaggggct cgagccgttc ctgtgccgtg
      841 ggctcgaggt gctcgattcg acgcccgagg agttcgcctc agcgctccgc agcgagaacc
      901 acacgctcaa gcgggctctg accgacccgc ggctgttcag cggcatcggc aacgcctatt
      961 cggacgagat cctgcaccgg gcccgcctct ccccgatgca gctcacgagc cgcctgggag
     1021 acgaagaggc ggcccgcctg catgccgcga ccgtcgcgac cttgcgcgac tggacggagc
     1081 ggctccggcg cgagacgggg gagggctttc ccgagaaggt cacggccttc cgcgagggca
     1141 tggcggtcca cggccgcttc gggcagccgt gccccgactg tggcacctcg gtgcagcgga
     1201 tcgtctacgt cgagaacgag tgcaactact gtccgcgctg ccagacggag ggtcgcctgc
     1261 tcgccgaccg cgccctgtcg cgcctgttga aggacgactg gccgaagacg atcgaagaga
     1321 tggaaggggt ctcgggcggc aggggatgac ggccgatccg ggggcctacc tggtcgctcc
     1381 gggtcctttg agcgcccggc cggggagcgc gtccgtgtgc cggccgtcct tcagcaccgg
     1441 gaccccgttc acgaggacgt gatgcactcc ttcggcgtac tggtgcggct tgtcgaaggt
     1501 cgcgcggtcg acgatggtgg ccggatcgaa cacggcgaca tcggcgaact ggccctcctt
     1561 cagaaagccg cgccggtcca ggccgaggtt cgtggccggc aagcccgtca gtcgtcgaac
     1621 cgcctcttcc aggctgatga gcttctcctc gcgcacgtag tgtcccagca gccgggcgaa
     1681 gttgccgtag gcgcgggggt gggcggagga cttgaggaac accccctccg gggacatcga
     1741 tgcggcgtca gacccgaagg agacccacgg gagctggatc tggcgccgga cgttgtcctc
     1801 cgacatcagg aagaaggcga cgccgacgcg ggtacggtcc tcgaggatca ggtccatgac
     1861 cacctcgggc cagtctttct tgcgctccca cgcaacctcc gcgaggcgct tcccctgcag
     1921 cggcttgaga gcagggttct tgaactcgag caaaagcatg ctcgcgggcg atcgcgccag
     1981 gttgcagatg ttctcccacc cctctgcggc ggacgccatc tccttgtaga tcatggcccg
     2041 ggacttgggg tcccgcaggc gcctgtagag cgcctcctgg tccccctccc gggcccaggg
     2101 gggcaggcag gcgtcgaagc cggtggcggc ggcggtatag gtgtacatgt cggcggtgat
     2161 cggcagcccc tcggctcggg ccgcctcgac cttggcgatg accgcgtcga gcttcggcca
     2221 gttctcctct cccgccgcct tcaggtggta gatctcggcc gggagccccg cctctctggc
     2281 gattagcagg acctcgtcca ctgcc
//
LOCUS       DASRIQ010000189         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1133307, whole genome shotgun sequence.
ACCESSION   DASRIQ010000189 DASRIQ010000000
VERSION     DASRIQ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133307"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2821
                     /locus_tag="VFD06_04720"
     CDS             <1..>2821
                     /locus_tag="VFD06_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN02870.1"
                     /translation="VIPDLFVPGGITDPPFGDPKPIQIPPPSTAKNIVAVGASLTDCF
                     TLFGATDCEGSLVTFSSRGPATPESLRMAPMLTAPGADADFGPFTTAVAVFRSNDNDN
                     LAPVQAVLDEGNTGTSFSSGYLTGAAAVLRDYFAQGFYPTGSRGPAGDRVSNLSGALV
                     KAALSASADFNEDGIGTEGETSPQVHTLRRTRCMDLGTAVGIMCNSEQGYGRPVLTNV
                     LPLTAWSDSFALHPSSGRPREYPAAGLLVFDRLATGEGLIDNGANTSRSHLFRVAGPA
                     TVPTASGGQAIAAAQLRITLAWTDRPSVAGSGGPLINDLDLRLEGPGPDSCLAPGDLR
                     PDGSACPAGSASDNVFYDGNNYGPLTSGDPSRMQWSLPRAAAQEKHDRWNPVEAIHLA
                     ADPDNDRSFGDSPLYAGLWRVTVKRGLGGTTPGSLTLTPPTPAQDPDQNEDDNNNGRL
                     DAGEDNNANGLLDMPGEPYALVVSGPVFAGEAPPPAGPQSFPASRLSFDRVQYDCGGG
                     AVLGLFDTTPGASAALASSATTVTVLDPMGAVLDTETGLPFSAPSPGSALSAPVPVRL
                     AGPAVSGNGILEADTGRTIRAVYAPSGQTEVEATASVRCTPDLIPENFTAMQGTGSQV
                     SVQGGCDNDPFPDAGEVVSYGIALRNRSRRDAYADVIATLTPSGPAAGAVRVLDSPKP
                     VGLLPDGGANGVFFQVYIDPAAIASLPAAQRIVTMRLDLDSSERGVRLGRQSYSFTHP
                     LNSDRDIRHYSTDLLTGGREVRDLNRSGVIDRPGEGDPALGYVLPSEDVTFSSLFSGS
                     GAPAGHFTNELGEDLDLNGAFTATVSLFERNVIPNVDGGGIPVLDRGILNSNNPADPN
                     HRAPWNFDNNAGGWIPFRHPESNAAGVNVNPIWKYRTTYHCGFQTAGGASKFGIWHSS
                     EFDEMPSTTCHHYALPHDPNTPAKVE"
BASE COUNT          415 a         1088 c          921 g          397 t
ORIGIN      
        1 gtgatccccg acctgttcgt gccgggggga atcaccgatc cccccttcgg ggaccccaag
       61 cccatccaaa tccctccgcc ctccacggcc aagaacatcg tcgcggtcgg tgcgtcgctg
      121 accgactgct tcacgctgtt cggagcgacc gactgcgagg ggtcgctcgt caccttctcg
      181 tcgcgcggtc cggccacccc cgaatcgctg cgcatggcgc cgatgctgac ggccccgggc
      241 gcggacgccg atttcggacc tttcaccacc gccgtggcgg tgttccggag caacgacaac
      301 gacaacctgg cgccggtgca ggcggtgctc gacgagggga acaccggcac gtcgttctcg
      361 tcgggatacc tgaccggcgc ggcggcggtc ctgcgcgact acttcgccca ggggttctac
      421 ccgaccggca gccgcggacc ggccggcgac cgggtgagca atctctccgg cgcgctcgtc
      481 aaggcggccc tctccgcctc cgccgacttc aacgaggacg gcatcgggac ggagggggag
      541 acgagcccgc aagtccacac cctgcgccgg acccgctgca tggacctcgg gacggcggtc
      601 ggcatcatgt gcaacagcga gcaggggtac ggacggccgg tcctgacgaa cgtcctgccg
      661 ctcaccgcat ggtccgactc cttcgcgctc catccgtcgt cgggccggcc gcgcgagtac
      721 ccggccgccg ggctcctggt gttcgatcgc ctcgccaccg gcgaggggct gatcgacaac
      781 ggcgcgaaca cctcgcggtc gcacctgttc cgggtggccg gccccgcgac ggtcccgacc
      841 gcttcgggcg ggcaggcgat cgccgccgcc cagctgcgca tcacgctcgc ctggaccgat
      901 cgcccgtcgg tcgcggggag cggcgggccg ctcatcaacg atctcgatct ccggctggag
      961 gggcccggcc ccgactcctg cctcgccccc ggcgacctgc gcccggacgg ctccgcctgt
     1021 cccgccggaa gcgcctcgga caacgtcttc tacgacggca acaactacgg gccgctgacc
     1081 agcggggatc cgtcccggat gcagtggtcg ctgccgcgcg ccgccgccca ggagaagcac
     1141 gacaggtgga acccggtgga ggcgatccac ctggccgccg atccggacaa cgaccgctcc
     1201 ttcggcgact cgcctctcta cgccgggctg tggcgggtga ccgtgaagcg cggcctcggc
     1261 ggcacgacgc ccggatcgct cacgctcacc cctcccactc cggcccagga cccggaccag
     1321 aacgaggacg acaacaacaa cggccggctc gacgcggggg aggacaacaa cgccaacgga
     1381 ctgctcgaca tgccgggcga gccgtacgcg ctcgtcgtgt ccggcccggt cttcgccggc
     1441 gaggcgccgc cgccggccgg gccgcagtcc ttcccggcca gccggctgtc gttcgatcgc
     1501 gtccagtacg actgcggcgg cggcgccgtc ctcggcctct tcgacacgac gccgggcgcg
     1561 tccgccgccc tcgcctcctc ggccaccacc gtcaccgtcc tcgatcccat gggcgcggtc
     1621 ctcgacaccg agaccggttt gccgttcagc gcgccgtcac cgggatccgc gctgtcggcg
     1681 ccggtgccgg tgcgcctggc gggaccggcg gtgagcggca acggcatcct cgaggcggac
     1741 accgggcgga cgatccgcgc tgtttacgcc ccctccggac agacggaggt cgaggcgacc
     1801 gcctcggtgc gctgcacccc cgacctcatc ccggagaact tcacggcgat gcaggggacc
     1861 ggctcgcagg tctcggtgca gggaggctgc gacaacgatc ccttccccga cgcgggggag
     1921 gtcgtctcct acgggatcgc acttcggaac cgctcgcgcc gtgatgccta cgccgacgtc
     1981 atcgccacgc tcaccccctc ggggcccgcg gcgggggcgg tgcgggtcct cgactcgccg
     2041 aagccggtcg ggctcctccc cgacggcggc gccaacggcg tcttcttcca ggtgtacatc
     2101 gaccccgccg cgatcgcatc cctgccggcg gcgcagcgga tcgtcacgat gcgcctcgac
     2161 ctcgactcct cggaacgcgg cgtccggctc gggcggcagt cgtactcctt cacgcacccg
     2221 ctgaactccg accgcgacat ccggcactac tccaccgacc tcctcaccgg cggccgcgag
     2281 gtgcgcgacc tgaaccgcag cggcgtcatc gaccggccgg gcgaagggga tccggcgctc
     2341 ggctacgtgc tgccgtcgga ggacgtcacc ttctcctcgc tcttctccgg cagcggcgcc
     2401 ccggcggggc acttcaccaa cgagctgggg gaggatctcg atctgaacgg cgcattcacc
     2461 gcgaccgtct ccctgttcga acgcaacgtg atcccgaacg tcgatggcgg cgggattccg
     2521 gtgctcgatc ggggcatcct caactccaac aacccggccg atccgaacca ccgcgctccg
     2581 tggaatttcg acaacaacgc cggcggctgg atccccttcc ggcatccgga gagcaatgcc
     2641 gcgggggtca acgtcaatcc gatctggaag tacaggacaa cctatcattg cggcttccag
     2701 acggccggcg gcgcgagcaa gttcgggatc tggcactcca gcgagttcga tgagatgccg
     2761 tccacgacct gtcatcacta cgcgctcccg cacgacccca acacccccgc caaggtggag
     2821 g
//
LOCUS       DASRIQ010000190         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_601004, whole genome shotgun sequence.
ACCESSION   DASRIQ010000190 DASRIQ010000000
VERSION     DASRIQ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601004"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2568)
                     /locus_tag="VFD06_04725"
     CDS             complement(<1..>2568)
                     /locus_tag="VFD06_04725"
                     /inference="COORDINATES: protein motif:HMM:NF026036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN02871.1"
                     /translation="SPDGRWILFTGHYGGAADVFIMPAEGGEPRRLTWHPGVDEACGF
                     TPDGKAVLFTSNRDTPFGSDSVVLRQFFTMPLGGGFPERLPVPTTRRGSYSEDGRLLA
                     YEQARWQEEWRLYRGGQAQPIRILNLSDLSMSAVPGPMSVNQHPVWLGGRLYFTSDRD
                     GKRMNLQEFDPASQAVRPLTRHEDFDVHSVSAGGGMLAYEQGGRIHTLDPKSGQDETL
                     VIRVRGDFPWAMPHWDDVAAQIADAGLSPGGARAVFEARGDIFTVPAEKGDVRNLTRS
                     PGAADHGPAWSPDGAKIAWFSDEGGEYRLLIADQDGIAPPRAVAIAPATYFFDLAWSP
                     DSKRVAFTDAARNVHVVEVATGRDTPIDTDRMAHPERSLVPVFSPDSRFLAYAKQLPN
                     QYRGIMVYSFDEGKARLVTDGLSDAVAPVWDKSGKYLYFLASTDLALGTGWLDLSSLE
                     RPVRRGVYCAVLARDLPSPLLPESDDEKPSEEEKAEGKGKGAAEGKKGSGEDKKPEPV
                     KVRIDFDGLLNRIVALPIPPRNYSGLSAGTEGVVFVAEQPDLFNDSDSPQPAASVHRF
                     DFAKRKETTFLSGPISTFTVAAGGKKALYHSGETWGIVATDAEPKLGDGALKVELQMQ
                     QDPQAEWRQIFLEAWRFERDFFYVPNHHGADWNAMKDRYGAWLPSVRHRADLSDLINR
                     MGGELGVGHHFVAGGDNPTIPGSRIGLLGADLKVEQGRYRIVRIFDGESWNPDLRAPL
                     AAPGVDVRKGDFLLAVNGREIRPPDTPYRLFEGTADKQTTITVNDKPLLEGARKVVVV
                     PVPGERGLRRRAWVEDNRRLVDRLSGGRLAYVWLPNTAADGYVYFNRYYFAQQDRPG"
BASE COUNT          365 a          911 c          891 g          401 t
ORIGIN      
        1 ccccgggccg atcctgctgg gcgaagtagt agcggttgaa gtagacgtag ccgtccgccg
       61 cggtgttcgg cagccagacg taggcgagcc ggccccccga gaggcgatcg accaggcgcc
      121 ggttgtcctc cacccaggcg cggcgccgca ggccccgttc ccccggcacc ggcaccacca
      181 cgaccttgcg ggccccttcg agcaacggct tgtcgttgac ggtgatcgtc gtctgcttgt
      241 ccgccgtccc ttcgaagagg cggtacgggg tgtcgggggg ccggatctcc cgcccgttga
      301 cggcgagcag gaaatccccc ttgcgcacgt ccacaccggg ggcggcgagc ggcgcccgga
      361 gatccgggtt ccaggactcg ccgtcgaaga tgcggacgat gcggtagcgc ccctgctcca
      421 ccttgaggtc ggcgccgagc aggccgatgc ggctgccggg gatggtcggg ttgtcgccgc
      481 cggcgacgaa gtggtgcccg acccccagct caccgcccat gcggttgatg aggtcgctca
      541 gatcggcgcg gtgccgcacc gacgggagcc acgcgccgta gcgatccttc atggcgttcc
      601 agtcggcgcc gtggtggttc gggacgtaga agaagtcccg ctcgaagcgc caggcttcga
      661 ggaagatctg ccgccactcc gcctgcgggt cctgctgcat ctggagctcg actttgagcg
      721 cgccgtctcc caatttgggt tcggcgtcgg tcgcgacgat cccccaggtt tccccggagt
      781 ggtacagcgc tttcttcccg cccgcggcca ccgtgaacgt cgagatgggg ccgctcagga
      841 aggtcgtctc cttgcgcttg gcgaagtcga agcggtgcac cgacgccgcc ggctgcggcg
      901 agtcggaatc gttgaacaga tccggctgct ccgcgacgaa caccaccccc tcggtccccg
      961 ccgacagccc cgagtagttg cgcggcggta tcggcagcgc gacgatccgg ttcaggagtc
     1021 cgtcgaagtc gatccgcacc ttgaccggct ccggtttctt gtcctccccg ctgcctttct
     1081 ttccttccgc cgcccccttc cccttgccct ccgccttctc ctcctccgac ggcttctcgt
     1141 cgtcgctctc gggaaggagc ggcgacggta ggtcgcgcgc cagcacggcg cagtagacgc
     1201 cgcggcgcac tggacgctcg aggctgctga ggtccagcca gccggtgccg agcgcgaggt
     1261 cggtgctggc gaggaaatag aggtacttgc ccgacttgtc ccagacgggc gcgacggcgt
     1321 cggagagtcc gtcggtcacg aggcgcgcct tcccctcgtc gaacgagtac accatgatgc
     1381 cgcggtactg gttcggcagc tgcttggcgt acgcgaggaa gcgcgagtcg ggggagaaga
     1441 ccggcacgag cgagcgctcc gggtgcgcca tccggtcggt gtcgatcggc gtgtcgcggc
     1501 cggtcgcgac ctccacgacg tgcacgttgc gcgcggcgtc ggtgaaggcg acccgtttcg
     1561 agtcggggga ccaggcgaga tcgaagaagt aggtcgccgg agcgatggcg acggcgcgcg
     1621 gcggcgcgat cccgtcctga tcggcgatca ggaggcggta ctcgccccct tcgtccgaga
     1681 accaggcgat cttcgcgccg tccggcgacc acgcggggcc gtgatcggcc gccccggggc
     1741 tgcgggtgag gttccggacg tcccccttct ccgccggcac cgtgaagatg tcgccgcggg
     1801 cctcgaacac cgcccgcgcc ccgccgggcg aaagacccgc gtcggcgatc tgcgccgcca
     1861 cgtcgtccca atgcggcatc gcccacggga agtcgccgcg gacccggatg accagcgtct
     1921 catcctggcc gctcttcgga tcgagggtat ggatccggcc cccctgctcg taggccagca
     1981 ttccgccccc ggccgagacc gagtgcacgt cgaagtcctc gtgccgcgtc agtggccgga
     2041 ccgcctggct cgccggatcg aactcctgca aattcatccg cttcccgtcg cggtcggacg
     2101 tgaagtagag gcggccgccg agccacaccg ggtgctggtt caccgacatg gggccgggga
     2161 cggcgctcat cgacaggtcg gagaggttca gaatgcggat cggctgcgcc tggccgccgc
     2221 ggtagagacg ccattcctcc tgccagcgcg cctgctcgta agccagcagg cggccgtcct
     2281 cgctgtacga gccgcgacgt gtcgtcggga ccggcagccg ctccgggaac ccgccgccca
     2341 gcggcatcgt gaagaactgc cgcagcacga ccgagtcgct gccgaacggg gtgtcacggt
     2401 tgctggtgaa gagcaccgcc ttcccgtccg gcgtgaagcc gcacgcctcg tcgacccccg
     2461 ggtgccacgt cagccggcgc ggctcccctc cctcggccgg catgatgaag acgtcggcgg
     2521 cgccaccgta gtgaccggtg aacaggatcc accgaccgtc gggggaga
//
LOCUS       DASRIQ010000191         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1134891, whole genome shotgun sequence.
ACCESSION   DASRIQ010000191 DASRIQ010000000
VERSION     DASRIQ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134891"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..648)
                     /locus_tag="VFD06_04730"
     CDS             complement(88..648)
                     /locus_tag="VFD06_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02872.1"
                     /translation="MSIHRPAAPFTGAAVFLAALCSGAAADFFPASAARSDAPPSARV
                     GAVRRIQSVCFAARPPSLLKGLTGHDAVPAEVRDDPARQMKAFVDVDRREKGLFYPTS
                     LDDVLDAFESEVRPGMRLLDLGSGDGRVVFLSALLGADATGVEWEESLHRIALESRDR
                     LADLVPKRRARLVVKVYIRPGRGSLP"
     gene            complement(694..>2555)
                     /locus_tag="VFD06_04735"
     CDS             complement(694..>2555)
                     /locus_tag="VFD06_04735"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HZN02873.1"
                     /translation="TWVCGDPGMQFDTTVNDWHPCPNTARINASNPCSEYMFLDDSAC
                     NLASLNLMKLTTPEGELDVTACKKAIETTILAQEIIVDNASYPTERIARNSHDYRPLG
                     LGYANLGALLMSRGLAYDSDTGRDYAGAITAVLTGHAYVMSARIASTMGPFNGYEANR
                     EPFLRVIRKHGSHVAKIDPAHVPLDLYTAARDAWNEAHELGSKHGIRNGQVTVIAPTG
                     TIAFMMDCDTTGIEPDIALIKYKKLVGGGMLKIVNQTVPKALARLGYSDQQTKEILES
                     LDENETIEGAPHLKDQHLTVFDCAFRPMRGSRSIHYMGHVKMMAAAQPFISGAISKTV
                     NVPADTTVDEIQQAYVESWKQGLKAVAIYRDGCKRSQPLNTGKDQAKTKIEAVSTAAV
                     AEAPRAVRRRLPDERKALTHKFSIAGHDGYLTVGMYEDGKPGEIFLVMAKEGSVVSGL
                     MDCFATAVSLALQYGVPLQVLVDKFSHVRFEPSGFTNNSEIPIAKSIIDYIFRWLGSK
                     FLGREAAEAIGVHVKPDAETAPEPQNVGHTAPGTAPLHMVPRHGGGPIPVALVPGPST
                     PSSVGAAGNDTSRAPFAFRPDEDAPPCPDCGSIMVRNGACYKCLNCGSTSGCS"
BASE COUNT          366 a          858 c          917 g          414 t
ORIGIN      
        1 gcggctcgag ccagaagccc cacaggccga ggagcgcgag gaggagcgcc accgccgcac
       61 cgaacaaccg gaccgggagg ggcgtgttca ggggagggag ccgcgtcccg gccggatgta
      121 gaccttgacg acgaggcggg cgcgccgctt cggaaccaga tcggcgagcc ggtcccggga
      181 ctcgagggcg atccggtgga gcgactcctc ccactcgacg ccggtggcgt cggcgccgag
      241 gagggccgac aggaacacca cccgcccgtc gccgctgccg aggtcgagga gcctcatccc
      301 cggccgcacc tccgactcga aggcgtccag gacgtcgtcg agggaggtcg ggtagaagag
      361 ccctttctcg cggcggtcca cgtccacgaa cgctttcatc tgccgcgccg ggtcgtcccg
      421 cacctcggcc gggacggcgt cgtggccggt gaggcccttc aggagtgacg gcggccgggc
      481 ggcgaagcag acgctctgga tgcgacggac cgctccaacc cgcgccgacg gcggcgcgtc
      541 ggaccgcgcg gcgctcgccg ggaagaagtc ggccgccgcg ccggagcaga gggcggccag
      601 aaaaaccgcg gcgcccgtga agggcgccgc agggcgatga atcgacatca taaccaccgg
      661 ccgatcgacg cgggtgcgcc ggcgagcccc gcctcaggaa cagccgctgg ttgagccgca
      721 gttgaggcac ttgtagcagg ccccgttgcg caccatgatc gagccgcagt cggggcacgg
      781 tggtgcgtcc tcgtccgggc ggaacgcgaa cggcgcgcgg ctggtgtcgt tcccggccgc
      841 tcccaccgac gacggcgtgg acggtccggg aacgagcgcc accgggatcg gccccccgcc
      901 atgacgcggg accatatgga gcggcgctgt tcccggcgcc gtgtgcccga cgttctgggg
      961 ttcgggcgcc gtctctgcgt ccggcttgac gtgcacgccg atcgcctccg ccgcctcgcg
     1021 ccccaggaac ttcgaaccga gccagcggaa gatgtagtcg atgatcgact tggcgatcgg
     1081 gatctccgag ttgttggtga acccggacgg ctcgaagcgg acgtggctga acttgtccac
     1141 gaggacctgg agcggcacac cgtactgcag cgccagggac accgcggtcg cgaagcagtc
     1201 catgagcccg gagacgacgc tgccttcctt cgccatcacg aggaagatct cgcccggctt
     1261 gccgtcctcg tacatcccga ccgtcaggta cccatcgtgc ccggcgatgg agaacttgtg
     1321 cgtcagcgcc ttgcgctcgt ccggcagccg gcggcgcacg gcgcgcggcg cctcggcgac
     1381 cgccgccgtc gagaccgcct cgatcttcgt cttcgcctga tccttgccgg tgttgagcgg
     1441 ctggctgcgc ttgcagccgt cgcggtagat cgccaccgcc ttgagcccct gcttccagga
     1501 ctcgacgtag gcctgctgga tctcgtccac cgtcgtgtcg gccgggacgt tgaccgtctt
     1561 gctgatggcg ccgctgatga acggctgcgc cgccgccatc atcttgacgt gccccatgta
     1621 gtggatgctg cggctgccgc gcatcgggcg gaaggcgcag tcgaagaccg tcaggtgctg
     1681 gtccttgagg tgcggcgccc cctcgatggt ctcgttctcg tcgagcgact cgaggatctc
     1741 cttggtctgc tggtcgctgt agccgagccg cgcgagcgcc ttcgggacgg tctggttgac
     1801 gatcttgagc atcccgccgc cgaccagctt cttgtacttg atgagggcga tgtccggctc
     1861 gatgccggtg gtgtcgcagt ccatcatgaa ggcgatggtg ccggtcggcg cgatgacggt
     1921 cacctggccg ttgcggatgc cgtgcttcga gccgagctcg tgcgcctcgt tccaggcatc
     1981 gcgcgccgcg gtgtagagat cgagcggcac gtgcgccggg tcgatcttcg cgacgtgcga
     2041 gccgtgcttc cggatgaccc tgaggaacgg ctcgcggttc gcttcgtacc cgttgaacgg
     2101 ccccatggtg gaggcgatgc gcgccgacat cacgtaggcg tgtccggtca gcacggcggt
     2161 gatcgcgccg gcgtagtcgc gcccggtgtc cgagtcgtac gccaggccgc gcgacatcag
     2221 gagcgccccg aggttggcgt agccgagccc gagcggccgg tagtcgtgcg agttcctggc
     2281 gatccgctcg gtcgggtagg aggcgttgtc gacgatgatc tcctgggcca ggatcgtcgt
     2341 ctcgatcgcc ttcttgcagg cggtgacgtc cagctcccct tccggcgtcg tcagcttcat
     2401 gaggttcagc gacgccaggt tgcaggcgga gtcgtcgagg aacatgtact cggagcacgg
     2461 gttgctggcg ttgatgcgcg ccgtgttcgg gcacgggtgc cagtcgttca ccgtggtgtc
     2521 gaactgcatg ccgggatcgc cgcacaccca ggtcg
//
LOCUS       DASRIQ010000192        13211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2699224, whole genome shotgun sequence.
ACCESSION   DASRIQ010000192 DASRIQ010000000
VERSION     DASRIQ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2699224"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /gene="larC"
                     /locus_tag="VFD06_04740"
     CDS             <1..474
                     /gene="larC"
                     /locus_tag="VFD06_04740"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HZN02874.1"
                     /translation="GALEVFYAPIQMKKNRPGTMVTIICPEPRLEAVTEVIFRETTTI
                     GFRYLPMGRIEMARRFDRVRTPWGPVRVKVSRYNGRVMQATPEYEDCRALALGASVPL
                     KEVQQAAQAEWAKGPGRAGAEGRRRPAAQRRARPRTRRTARTGERRRTRRRGGGR"
     gene            471..770
                     /locus_tag="VFD06_04745"
     CDS             471..770
                     /locus_tag="VFD06_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02875.1"
                     /translation="MIYDRDKAARMLKVVVDITAGAIDLATRQGGDPGVRQPALAAEK
                     TMRTLAAKGDCDGMYHEYLRLLQDAEHLGRPLDRVHQKSPESELYRFVQAYWERE"
     gene            796..2808
                     /gene="metG"
                     /locus_tag="VFD06_04750"
     CDS             796..2808
                     /gene="metG"
                     /locus_tag="VFD06_04750"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZN02876.1"
                     /translation="MTRPIYFTTPIYYVNDAPHIGHTYTTVVTDVLARFHRMRGRDVR
                     FLTGSDEHGQKIERAAAARGLEPLALADEMVARYRALWSALGIAPDDFIRTTEPRHRK
                     GVEALYAKIKARGDIYKDRYAGWYCTSCESFYPENQVVDGRCPDQGHKVEWTEEESYF
                     FRLSKYQEPLLNLYRERPDFVFPETRRNEVVAFVAAGLKDLSISRSSFRWGLPFPDDP
                     KHVLYVWFDALSNYITALGYGAPDASLYGRYWPGIHLVGKDILRFHAVYWPAFLLAAG
                     EPPPRQIVAHGWWLRDAVKISKSRGGVVDPLPFIRDFGTDALRYVLLREMVFGQDASF
                     SDEAFIDRVNTDLANDLGNLQSRALKMIEDYRGGVLPAHSGGDGDAALRAAAQGAWDG
                     LVDGFESYALQAGLQRVSDLVGALNRHIVQHEPWKMARDPAQARRLDDLLYAIAEGLR
                     IAAVLLAPVIPRAAQTLYASVGGEGEVSGVSLASFQWGLLSPGSRIVRGQPLFPRIDK
                     AAHLKAAVQTADKAPIQEQRMETPDNRKDTPPAPAPAAPGMPPTAAAAPSAGPAEIGI
                     DDFARVELRTAKVLEAERVAGADRLLKLTVDIGTEKRTIVAGIAARYAPEALIGKTIV
                     VVANLKPAKLRGVVSQGMLLAASDASGAPYILTTEDPGVPAGWRVK"
     gene            2825..3619
                     /locus_tag="VFD06_04755"
     CDS             2825..3619
                     /locus_tag="VFD06_04755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZN02877.1"
                     /translation="MPPAWSDSHAHLTDERFADDLDAVIDRARAAGLARLVTVASRLG
                     DGEACADLAGRHDFVTFAVGLHPHEAKHWSLETVRGIERTARRNKCIAIGEIGLDYHY
                     NLSPPKEQRAAFREQIVLARGLGLPIVLHTRSAWDDTFAILRDENAASLGGAFHCFSG
                     GPEEAHKALATGFLLSFAGPLTFKNADALREAAAMVPADRLLIETDAPYLTPHPHRGE
                     RNEPARVGLVGERLAAIHDVAPDALAATVTANLERLFRIAEARSAT"
     gene            3680..3958
                     /locus_tag="VFD06_04760"
     CDS             3680..3958
                     /locus_tag="VFD06_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02878.1"
                     /translation="MRKVAVEPVEEQAALLADLLESAGFTALLAWIDDGGHPHIIPRQ
                     QSVAPAAGLLPPVTNPFAVYIPEEDAEEALQVLRDAGRAGVTDAPFAS"
     gene            complement(3933..4418)
                     /locus_tag="VFD06_04765"
     CDS             complement(3933..4418)
                     /locus_tag="VFD06_04765"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HZN02879.1"
                     /translation="MSSAYRVFFICTGNSARSQMAEGLANHLGQGRLEAHSAGTYPVG
                     VNSHAIEVMREHGIDISNHYSKRIDEVPGPFDLVITLCDNAALLCPVSVRDYPMEHWS
                     TPDPSFVPGGSNAVHQAFRDVRDRLEGQIRDLVTRIEGEETAKPPATFTPGVTKRTAR
                     P"
     gene            4517..6337
                     /locus_tag="VFD06_04770"
     CDS             4517..6337
                     /locus_tag="VFD06_04770"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZN02880.1"
                     /translation="MSRGGGRRVLAGGAAAGAAARTPGAGTPAAADAFDRLITEDWEW
                     RLADCPPYATLVGDPRYDDRWMDLSFEAFDRRASRYRSFLAHLEATDTAALDPKRRLD
                     HRLLLEETRLNVESLRFPEELQPLSQISGAHQEAAEVMQFTPRGDATARERIVQRLRA
                     VGDLVEQTIALMRRGAQRGITPPRAILAPVPAQIAKQIAADPDDTPVARILFAEPPPG
                     AAGEGWSRFQRDVRRAIESVVTPAHRRLDEFVRREYLPRTRVTTGLAHLPDGEAWYAH
                     LIRRYTSTELDAKAIHALGKSEVARLRAEMKKTLRRAGHRGDLASYFRHLRTDRRYFF
                     ARREDLLTAYRDLCKRVDASLPRLFKTLPRLPYGVAPVPAYSEKEQTTAYYQPGSQEA
                     GRAGLFYANTYNLKARPKWEMEALTAHEAVPGHHLQIALAQELADLPAFRRHGHHTAF
                     VEGWGLYAESLGEELGLYRDPASAFGRLTYEMWRAIRLVVDTGLHAFGWSRERAVRYF
                     EENAGKAGHDIAVEVDRYLAWPGQALAYKLGELTIKDLRERARRAHGARFDVRDFHDV
                     VLGTGPVPLSVLKTRVEDWIATVPAEQPPPRGPRRAARSL"
     gene            6334..7233
                     /locus_tag="VFD06_04775"
     CDS             6334..7233
                     /locus_tag="VFD06_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN02881.1"
                     /translation="MRSASRVLAGVLATALAGAAATGAPARHPTAASRIENRSATVHG
                     LKIHYLEAGYGQPLVLLHGLGGSAESMKPLVAVLARRHHVYAPDQIGFGGSDKPLIDY
                     RITTLVDFLHGFFATLGIERADLVGHAVGARVASLYALEHPERIGRLVLISGAGYRPD
                     LDEEAERALTFATLADARRALTLAYADDGRATQAAAERLFSTRLKSGSAWTVARLVES
                     FRSGEGWADDLSPIQAPTLIVWGRDDELAKVGVAERAHRQIRGSRLVLLDHCGHLPLE
                     EQPKALESALTSFLDPVAGPASR"
     gene            7406..9424
                     /locus_tag="VFD06_04780"
     CDS             7406..9424
                     /locus_tag="VFD06_04780"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HZN02882.1"
                     /translation="MTSRILRQFSGVIAAVVSAFFAAIPSVPCAAAEAREAGATTGGP
                     ALDPGTGSLYWRSSDGLVPLPALAIDADLRVTGLLVQGTVRQTFRNTSGRTIEALYVF
                     PLPERASVDAFEMRLGDRVVHSEVRERDTARAAYEAAKQEGRRTALLEFTRKGLFRTS
                     IAGIAPDEEIAVTLRYFDEAEDRDGGFALALPLTWRPRYRPGAERRPDETFVPVSSRS
                     APAVEVRVAIEAGHPVAAVRSASHPIRVNGDLHAGSLQAATLPGVPADRDFRLEWTLE
                     SASQPRAAVFLEEREDGPYGLVMIVPPDDGAAHDDGLSTATLFVVDVSGSMEGPSLAQ
                     AKRALLAALDALRPGDSFNLLKFSDGSAPFRADYQPATPSRLDEARDWIRGLEVEGGT
                     EILAALRHAQALVETAPERGARRVILITDGAADADDEAIAAITRGFGDARLHAVGIGP
                     APNRPLMRSLARRGRGACEFIGSLEEVASRLDGFLDRLRRPVLTDLVLDWNGVPPLDV
                     RPSPLPDLYAGEPLVLSFRAASGPVPIGMTLRGATASGPFSTSVEIAPDAPRGAGLGA
                     RWARARIEDLLDGLAAGSAEEQVREAVVPLALEFGLVTPYTSRVAIEERITYRGATPV
                     AAANALPGSAGDEITLPGTGTLDPLWLRCGLLLIAAGLMFVCARRWLA"
     gene            9421..10071
                     /locus_tag="VFD06_04785"
     CDS             9421..10071
                     /locus_tag="VFD06_04785"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HZN02883.1"
                     /translation="MTAAVAGRPPKRVAAILAAASAALLAAGAALVGRHLYLEVKAVA
                     AGVLIDRAWAGRLDGGRTLRPWSWADFAPIARLESRRLGIDRPILSDASGRTLAFGLG
                     HLPGSAPPAETGTCVVAGHRDTWAAFLRDLRTGDEVLIRIPRGARRYRVAGATVVDAG
                     DLRLTAGLEPTAPTDRLLLTTCWPFGALRRGPLRYVVICEAIDGLPSIALNRRPTR"
     regulatory      10108..10275
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10290..11549
                     /locus_tag="VFD06_04790"
     CDS             10290..11549
                     /locus_tag="VFD06_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02884.1"
                     /translation="MGRPTVPAARRFRRAAAPFTTFVALLASAASGATAGAVDCFPDR
                     VALYAPGPVSSPPPFNSWQPGIVLGPPGNTTPTNGTLTVVSLGRGGRIVLEFTDNVIV
                     DGPGPDFVVLENPFFCTAAPLTAADPWSSFAEPGIVEASEDGVVFHAFPYDPAALAQV
                     TSICSDRTVIAALRGLAGLTPSFTGDWTVPDDPDVFDAAAPGGVSGHGGDAFDLQSVG
                     LAQARFLRITDANLAIGPPGASEGFDLDAVVALHAVALDAGPDADGDGLSDATETQVY
                     GTSPSDPDSDGDGTSDGVEAATCRAPLGASTAPSFAPSLDLEVFEAQPTMVRWNTLGP
                     GNSYDLVRGSTAALHPVGGMIDLGVVACIENDSTDLSNRTLGDAAIPPAGQAFFYLAR
                     PTPRGTPPGYGLSSSGLPRVPASGDCQ"
     gene            11546..>13211
                     /locus_tag="VFD06_04795"
     CDS             11546..>13211
                     /locus_tag="VFD06_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN02885.1"
                     /translation="MINAPGGADPRRLRSVAAALLVAAAFLRASSGAFAGEDSVEDGA
                     APPAPPAEAGPSPEPDAGSSAPSVPEETITVRAPTDVLRADPTAFGTVVRKEQWADRI
                     TTIPDLLREVVGVQVKSLGDSFSTVSIRGSTAEQVMVYLDGVPLNRGAGGAVNLADIP
                     LAQVERIEVYRGMTPSSLPEASIGGAILIHTRAPKGRFAADGYAGLGSYGSGEFSAGF
                     GGEKGRGGWRIATDGGTSDGDFPFWDNNGTPDDPADDGVAPRVNNDFTSGHVLARGHL
                     RAAETLFTFEADAFRREQGIPGVDSVQSEGARLETARLLLAARAERAGLLSGRLVLHG
                     GLTRHDESEEFNGQGTHFVFGANTDNRYISTSLSGGGTLVAGSRQAIAFLASLRDETA
                     DLRDPSPGEGQVGFAERRILAATLEDQIVLAAGRVQIVPSLRFESSASDYAPGEAGGG
                     VSGSGDVDSDEVTGRIGARWDLSAAWSIRGNAGNYHRLPDITELYGHRGGVVGNPGLL
                     PERGTNADLGLTWQAVQPIGWFRTMRVEGVLFGTDAEDLIVYEPTPS"
BASE COUNT         1804 a         4889 c         4650 g         1868 t
ORIGIN      
        1 ggcgccctcg aggtgttcta cgcgccgatc cagatgaaga agaaccggcc cgggacgatg
       61 gtgacgatca tctgccccga gccgcgcctc gaggcggtga ccgaggtgat cttccgggag
      121 acgaccacca tcggcttccg ctacctgccg atggggcgca tcgagatggc ccggcgcttc
      181 gaccgcgtcc ggacgccgtg gggccccgtg cgcgtcaagg tctcgcgcta caacggccgc
      241 gtcatgcagg cgacgcccga gtacgaggac tgccgcgcgc tggcgctcgg cgcgtccgtg
      301 ccgctcaagg aggtccagca ggcggcgcag gcggagtggg cgaaggggcc cggccgcgcg
      361 ggcgcggagg gccggcgccg gcctgccgct caacgccggg cgcggccacg gacgcggcgc
      421 accgcgcgta caggagaacg gcgccgcacc aggaggcgtg gagggggacg atgatctacg
      481 atcgtgacaa ggcggcccgc atgctgaagg ttgtggtcga catcaccgca ggggcgatcg
      541 acctggccac gcggcaggga ggcgatcccg gcgtgcgcca gccggcgctc gccgccgaaa
      601 agaccatgcg caccctcgcc gccaagggag actgcgacgg catgtaccac gagtacctgc
      661 gtctcctcca ggatgcggag cacctcggcc ggccgctcga tcgggtgcac cagaaatcgc
      721 ccgagtcgga gctgtaccgg ttcgttcagg cctactggga acgggagtga actgctgacg
      781 ccagatgggg cccctgtgac ccgcccgatc tacttcacca cgccgatcta ctacgtcaac
      841 gacgctccgc acatcggcca cacctacacg accgtcgtca ccgacgtcct ggcccgcttc
      901 caccgcatgc gcgggcgcga cgtccgcttc ctgaccggca gcgacgagca cggacagaag
      961 atcgagcggg cggcggcggc gcgcgggctc gagccgctgg ccctcgccga cgagatggtg
     1021 gcgcgctatc gcgccctgtg gtcggccctc gggatcgcgc ccgacgactt catccgcacc
     1081 accgagccgc gacaccggaa gggggtcgag gccctgtacg cgaagatcaa ggcgcgcggg
     1141 gatatctaca aggaccgcta tgccggctgg tactgcacct cctgcgagtc gttctacccg
     1201 gagaaccagg tcgtggacgg ccgctgcccc gaccaggggc acaaggtgga gtggaccgag
     1261 gaggagtcgt acttcttccg gctgtcgaag taccaggagc cgctcctgaa cctctaccgc
     1321 gagcgccccg acttcgtctt cccggagacg cggcgcaacg aggtggtcgc cttcgtcgcc
     1381 gccgggctca aggacctgtc gatcagccgc tcgtcgttcc gctggggtct ccccttcccc
     1441 gacgatccga agcacgtcct gtacgtctgg ttcgacgcgc tcagcaacta catcaccgcg
     1501 ctgggctacg gcgcccccga cgcgtcgctg tacgggcgct actggccggg gatccacctc
     1561 gtcggcaagg acatcctgcg ctttcacgcg gtctactggc cggcgttcct gctcgccgcc
     1621 ggcgagccgc cgcccaggca gatcgtcgcg cacggctggt ggctgcgcga cgcggtgaag
     1681 atctcgaagt cgcgcggggg cgtcgtggac ccgctgccgt tcatccgcga cttcgggacc
     1741 gacgcgctgc gctacgtgct gctgcgcgag atggtcttcg gccaggacgc ctcgttctcg
     1801 gacgaggcgt tcatcgaccg cgtgaacacc gatctcgcca acgacctcgg caacctccag
     1861 agccgcgccc tgaagatgat cgaggactat cgtggcggcg tcctgccggc ccactcgggc
     1921 ggggacggcg acgcggcgct gcgcgcggcg gcgcaggggg cgtgggacgg gctcgtggac
     1981 gggttcgagt cgtacgccct gcaggcgggg ctgcagcgcg tgtccgatct cgtcggcgcc
     2041 ctcaaccgtc acatcgtgca gcacgagccc tggaagatgg cgcgcgatcc cgcccaggcg
     2101 cgccgtctcg acgacctgct ctacgccatc gccgagggac tgcggatcgc cgcggtcctc
     2161 ctggcgccgg tgattccccg ggcggcgcag acgctctacg cttccgtcgg gggcgagggg
     2221 gaggtctcag gcgtctccct cgcatcgttc caatggggcc tcctttcgcc cggctcgcgc
     2281 atcgtgcgcg gccagcccct cttcccacgc atcgacaagg cggcgcatct caaggccgcc
     2341 gtgcagacgg ccgacaaggc gccgattcag gagcaacgca tggagacccc cgacaatcgc
     2401 aaggacactc ccccggcccc ggcccccgcc gcgcccggga tgccccccac cgccgcggcc
     2461 gcgccgtctg cgggaccggc ggagatcggg atcgacgact tcgcccgcgt cgagctgcgc
     2521 accgcgaagg tgctggaggc ggagcgggtg gccggggcgg accgcctcct caagctgacc
     2581 gtggacatcg gcaccgagaa gcgcaccatc gtcgccggca tcgccgcgcg ctacgccccg
     2641 gaggccctca tcggcaagac gatcgtcgtg gtggcgaacc tcaagccggc gaagctgcgc
     2701 ggcgtcgtgt cgcaggggat gctgctcgcc gcgtccgatg cctccggggc gccgtacatc
     2761 ctgacgaccg aggaccccgg cgtcccggcc ggctggcggg tgaagtagga ccccaggagc
     2821 ctgcgtgccg ccggcctgga gcgacagcca cgcccacctg accgacgagc gcttcgccga
     2881 cgacctcgac gcggtgatcg atcgcgcccg cgccgccggc ctcgcgcggc tcgtcacggt
     2941 ggcgtcgcgg ctcggggacg gcgaggcgtg cgccgatctc gccgggcggc acgacttcgt
     3001 cacgttcgcg gtcggcctcc acccgcacga ggcgaagcac tggtcgctcg agacggtgcg
     3061 ggggatcgag cggaccgccc ggcggaacaa gtgcatcgcc atcggcgaga tcggtctcga
     3121 ctaccactac aacctgtcgc cgccgaagga gcagcgggcc gccttccggg agcagatcgt
     3181 cctggcgcgc gggctcggac tgccgatcgt cctgcacacc cgctccgcct gggacgacac
     3241 cttcgccatc ctgcgggacg agaacgccgc ctcgctcggg ggggcgttcc actgtttctc
     3301 cggcgggccc gaggaggcgc acaaggcgct cgccaccggg ttcctcctgt cgttcgccgg
     3361 cccgctgacg ttcaagaacg ccgacgccct ccgggaggcg gcggcgatgg tgccggcgga
     3421 ccgcctgctg atcgagaccg acgcccccta cctcacgccg catccgcacc gtggggagcg
     3481 caacgagccg gcgcgcgtgg ggctcgtggg ggagcgcctg gcggcgatcc acgacgtcgc
     3541 tccggacgcg ctggcggcca cggtcaccgc gaacctggag cgcctgttcc ggatcgcgga
     3601 ggcgcgctcc gcgacctgac gcgcgttgac gaggccgagg gagccggtct agcatcgacg
     3661 catggatccc ttccaaggca tgcgcaaagt agcggtggaa ccggtggagg agcaggcggc
     3721 gctgctcgcc gatcttctgg agagcgccgg cttcacggcg ctgctcgcct ggatcgacga
     3781 cggcggtcac ccgcacatca tcccgcgcca gcagagcgtc gcaccggcgg ccgggctgct
     3841 tccgccggtg acgaatccct tcgcggtcta cattcccgag gaggacgcgg aggaggcgct
     3901 ccaggtcctg cgcgacgcgg gccgcgccgg agtcacggac gcgccgttcg cttcgtgacg
     3961 ccgggggtga acgtcgcggg aggcttcgcc gtctcctccc cctcgatccg ggtgaccagg
     4021 tcgcggatct gcccctcgag ccggtcccgc acgtcccgga acgcctgatg gacggcgttc
     4081 gatccccccg gcacgaagct cggatcgggg gtggaccagt gctccatcgg ataatcccgc
     4141 accgacacgg ggcacaggag cgcggcgttg tcgcagagcg tgatcaccag gtcgaacggc
     4201 cccggcacct cgtcgatccg cttggaatag tggttcgaga tgtcgatgcc gtgctcgcgc
     4261 atcacctcga tggcgtggct gttgaccccg accggatagg tgccggcgct gtgcgcctcg
     4321 aggcgtccct gcccgagatg gttcgccagt ccctcggcca tctggctgcg cgccgagttg
     4381 ccggtgcaga tgaagaaaac gcggtacgca gacgacatgg acgttcaccc tccccgagcc
     4441 atgtcttcac tccggcacgc attgtaatgc acacccgctc gccggacagg ttctcgatcc
     4501 gtcgcgcgga ggcacgatga gccgcggcgg gggccgccgc gtgctcgccg gcggcgcggc
     4561 agccggagcc gccgcgagga cgcccggcgc ggggacgccc gccgccgcgg acgccttcga
     4621 tcgcctgatc accgaggact gggagtggcg tctcgccgac tgcccaccct acgccaccct
     4681 cgtcggcgat ccgcgctacg acgatcgctg gatggacctg tcgttcgagg ccttcgaccg
     4741 tcgggcgagt cgttaccggt cgtttctcgc gcacctcgag gccaccgaca cggccgccct
     4801 cgatcccaaa cgccgcctcg accaccggct cctcctcgag gagacgcgcc tcaacgtcga
     4861 gagcctgcgc tttccggagg agctgcagcc gctgtcgcag attagcgggg cgcaccagga
     4921 ggctgcggag gtgatgcagt tcaccccgcg cggcgacgcc acggcccggg agcggatcgt
     4981 ccagaggctg cgcgcggtcg gagacctcgt ggaacagacc atcgcgctga tgcggcgggg
     5041 ggcccagcgc ggcatcacgc cgccacgggc gatcctggcg ccggtgccgg cccagatcgc
     5101 gaaacagatc gccgccgatc cggacgacac gccggtcgcg cgcatcctct tcgccgaacc
     5161 gccccccggc gcggccggcg aaggatggag ccggttccag cgggacgtgc gccgcgccat
     5221 cgaatcggtt gtgacgccgg cgcaccggcg gctcgacgag ttcgtccgcc gcgagtacct
     5281 gccacggacg cgcgtcacaa cagggctcgc tcatctcccc gacggggagg cctggtacgc
     5341 gcatctcatc cggcgctaca cctcgaccga gctcgacgcg aaggcgatcc acgccctcgg
     5401 gaagagcgag gtcgcgcgtc tgcgcgcgga gatgaagaag acgctgcgcc gcgccgggca
     5461 ccgcggcgac ctggcctcgt acttccgtca cctgcgcacc gaccggcgct atttcttcgc
     5521 ccggcgggag gacctgctca ccgcctaccg cgatctgtgc aagcgcgtgg acgcgagcct
     5581 gccgcggctc ttcaagaccc tgccccggct gccgtacggc gtggcgccgg ttccggcgta
     5641 ctccgaaaag gagcagacga ccgcctacta ccagcccggc tcccaggagg cgggacgggc
     5701 cggcctcttc tacgccaaca cctacaacct caaggcgcgg ccgaaatggg agatggaggc
     5761 gctcacggca cacgaggcgg tccccggcca ccatctccag atcgccctgg cgcaggagct
     5821 cgccgatctc cccgcgttcc gccggcacgg gcaccacacg gcgttcgtcg agggttgggg
     5881 gctgtacgcc gagtcgctcg gcgaggagct ggggctttac cgcgaccccg cctccgcctt
     5941 cggccggctg acctacgaga tgtggcgcgc catccgtctg gtcgtcgaca ccggcctgca
     6001 cgccttcggc tggagccggg agcgggcggt gcgctacttc gaggagaacg ccggcaaggc
     6061 ggggcacgac atcgccgtgg aggtcgatcg ctatctcgcc tggccggggc aggcgctcgc
     6121 ctacaagctg ggagagctga ccatcaagga tctccgggag cgggcgcgac gcgcgcacgg
     6181 agcgcgtttc gacgtgcggg acttccacga cgtcgtcctc gggacggggc cggtgccgct
     6241 ctcggtcctg aagacgcggg tggaggactg gatcgccacg gtgccggcgg aacagccccc
     6301 gccgcgcggg ccacgccggg ccgcgcgctc cctgtgagat ccgcttcccg ggtgctggcg
     6361 ggcgtgctcg ccacggcgct cgcgggcgcc gccgcgaccg gcgcccccgc gcgccacccg
     6421 acggcggctt cgcggatcga gaatcgcagc gccacggtcc acgggctcaa gatccactac
     6481 ctcgaggccg gctacggcca gccgctcgtc ctcctgcacg ggctcggcgg atcggcggag
     6541 tcgatgaagc cgctggtggc ggtcctggcg cggcggcacc acgtgtacgc cccggatcag
     6601 atcggcttcg gaggatcgga caagccgctc atcgactacc gcatcacgac cctcgtggac
     6661 ttcctgcacg ggttcttcgc gaccctcggc atcgagcgcg ccgacctcgt cggccacgca
     6721 gtcggggcgc gggtcgcgtc gctctatgcc ctggagcatc ctgagcggat cgggcgcctg
     6781 gtgctcatca gcggcgcagg ctaccggccg gatctcgacg aagaggccga acgggcgctg
     6841 acgttcgcca cgctcgccga cgcccgccgg gcgctcacgc tcgcctacgc ggacgacggc
     6901 cgcgcgacgc aggcggccgc cgagcggctt ttctcgacgc gcctgaagag cggcagcgcc
     6961 tggaccgtgg cgcgcctcgt cgagtcgttc cgctccggcg agggatgggc cgacgatctg
     7021 tcgccgatcc aggcgccgac gctcatcgtc tgggggcgcg acgacgagct cgccaaggtc
     7081 ggggtggcgg agagggccca caggcagatc cgcggctccc ggctcgtcct cctcgaccac
     7141 tgcggccacc tgccgctgga ggagcagccg aaggcgctcg agtcggcgct gacgtcgttc
     7201 ctcgatccgg tcgccggccc cgcttcgcgg taggacgcga ccgcttccgg cccgcatcgg
     7261 cggccgtgtc gccgcggaca cggatcgtgt catcccctcc acgtgcctgc cccgccggac
     7321 acaggcctga ggccgccatc gggcgtttcc gcgcggcaca gttcctgcgc tgagggcgcc
     7381 cgtaccccct cgaggaggag acgcgatgac ctcacgaatc ctgcgacagt tcagcggagt
     7441 gattgcggcc gtcgtttcgg cgttcttcgc ggcaatcccc tccgtcccct gtgcggccgc
     7501 cgaggcgcgc gaagcgggcg cgaccacggg cggaccggcg ctcgacccgg gcaccggctc
     7561 actgtactgg cgctcctcgg acggcctcgt gcctctcccc gccctcgcga tcgacgccga
     7621 cctgcgcgtg accggcctgc tggtccaggg gacggtgcgc cagacgtttc gcaacacctc
     7681 cggtcgcacg atcgaggcgc tgtacgtctt cccgctgccg gagcgcgcct cggtcgacgc
     7741 gttcgagatg cggctcggcg accgtgtcgt ccattccgag gtgcgcgagc gcgacacggc
     7801 gcgcgccgcc tacgaagccg cgaagcagga ggggcgccgg acggccctgc tcgaattcac
     7861 ccgcaagggg ctgttccgga cctcgatcgc cgggatcgcg cccgacgagg agatcgccgt
     7921 cacgctgcgc tacttcgacg aggcggagga ccgcgacggc ggcttcgcac tcgccctgcc
     7981 gctcacctgg aggccgcggt accgacccgg cgcggagcgg cggccggacg aaaccttcgt
     8041 gccggtctcg agccgatcgg cgccggccgt cgaggtgcgc gtcgccatcg aggcgggcca
     8101 ccccgtggct gccgtccggt ccgcctcgca cccgatccgc gtgaacggcg accttcatgc
     8161 gggatccctt caggccgcca cgcttcccgg cgttccggcc gaccgggact tccgactgga
     8221 atggacgctc gagagcgcgt cgcagccgcg ggccgcggtc ttcctcgagg agcgcgagga
     8281 cggcccgtac ggcctcgtca tgatcgtgcc gcccgatgac ggcgcggcgc acgacgacgg
     8341 attgtccacc gcgacccttt tcgtcgtgga cgtctccggc tccatggaag gaccgtccct
     8401 ggcgcaggcg aagcgggcgc ttctggctgc gctcgatgcg ctgcgccccg gggactcgtt
     8461 caacctcctg aagttctcgg acgggagcgc cccgttccgc gccgactacc agcccgccac
     8521 accctcccgg ctcgacgaag cccgcgactg gattcgcgga ctcgaggtgg aaggagggac
     8581 ggagatcctg gcggcgctgc gtcatgcgca ggccctcgtc gagaccgcgc cggagagggg
     8641 ggcgcggcgc gtcatcctga tcacggacgg cgcggcggac gcggatgatg aggcgatcgc
     8701 cgccatcacc cgcgggttcg gcgacgcccg cctgcacgcc gtcggcatcg gccccgcgcc
     8761 caaccggcct ctcatgcgct cgctggcgcg ccgcggccgg ggcgcctgcg agttcatcgg
     8821 gagcctcgag gaggtggcgt cgcgcctcga cggcttcctc gatcgcctgc ggcgcccggt
     8881 gctgaccgac ctggtcctcg actggaacgg cgtgcccccg ctcgacgtcc gcccgtcgcc
     8941 gctccccgac ctgtacgcgg gggagccgct cgtcctctcg ttccgcgccg cgtccggccc
     9001 cgttccgatc ggaatgaccc tgcgcggtgc caccgcctca gggcccttct cgacgagcgt
     9061 cgagatcgcc cccgacgctc cgcgcggcgc cggcctgggg gcgcgctggg cccgggcccg
     9121 gatcgaggat ctcctcgacg gcctcgccgc cggctcggcc gaggagcagg tgcgcgaagc
     9181 ggtggtcccc ctggcgctcg aattcggtct ggtcaccccc tacacgagcc gggtggcgat
     9241 cgaagagcgg atcacctacc gaggcgcgac gccggtcgcc gcggccaacg ccctccccgg
     9301 atccgccggc gacgagatca ccctgccggg caccgggacg ctcgatcccc tgtggctgcg
     9361 ctgcggcctg ctcctcatcg cggcgggact gatgttcgtc tgcgcccggc ggtggctcgc
     9421 gtgacggctg ccgtggccgg acggccgccg aagcgagtgg ccgccatcct ggcggccgcc
     9481 tcggccgcct tgctcgcggc tggggcggcg ctcgtcggcc ggcacctcta cctcgaggtg
     9541 aaagcggtcg ccgccggggt gctgatcgat cgtgcctggg cggggcgcct cgatggcggg
     9601 cggaccctgc gaccatggtc ctgggccgac ttcgccccga tcgcgcggct cgagtcgcgt
     9661 cgcctgggga tcgatcgccc gatcctctcg gacgcgagcg gccgcactct ggccttcggt
     9721 ctcggccatc tccccggaag cgcgccgccc gcagagaccg gcacgtgcgt cgtggcaggg
     9781 catcgcgaca cctgggcggc cttcctgcgc gatctgcgga cgggagacga ggtcctgatc
     9841 cgtatcccgc gcggcgcccg ccgctatcgg gtcgcgggcg cgacggtcgt cgatgcgggt
     9901 gacctccgcc tcacggccgg cctcgaaccc acagcaccga cggatcgcct gctcctcacc
     9961 acatgctggc ccttcggcgc gctgcgccgc gggccgttgc gctacgtcgt gatctgcgaa
    10021 gcgatcgacg ggctgccgtc gattgccctc aatcgccggc cgacgcgttg acagccggag
    10081 cgccgcgcgg gtataagacc tcgtcgtctt tgaaaaatcg tcgtgggtgc gggaaggggg
    10141 tgcaactccc ccgctgcccc gcaactgtga gcggtagcga acgccggaga atcccactgg
    10201 gggctcgtcc cctgggaagg gccggtcagt agaaagccgc gagccaggac acccaccctc
    10261 gacgcgcttc acctcgaggg aggatgtcca tgggacgacc caccgttccc gccgcccgcc
    10321 gtttccggcg cgccgcggcc ccgttcacga cgttcgtcgc gctgctcgct tccgccgcgt
    10381 cgggagctac ggccggcgcg gtcgactgct ttcccgatcg ggtggcgctc tacgcgccgg
    10441 gcccggtctc ctcaccaccc cccttcaact catggcagcc ggggatcgtc ctcggccccc
    10501 cgggcaacac gaccccgacc aacgggacgc tgacggtcgt gtccctgggt cggggcggcc
    10561 gcatcgttct cgagttcacc gacaacgtca tcgtggacgg cccgggcccg gatttcgtcg
    10621 tcctcgagaa cccgttcttc tgcaccgccg ccccgctgac cgcggccgac ccgtggtcgt
    10681 cgttcgccga gcccgggatc gtcgaggcga gcgaggacgg cgtcgtcttt cacgcgtttc
    10741 cgtacgaccc ggccgccctt gcgcaggtga cgtcgatctg cagcgatcgc acggtcatcg
    10801 cggcgctgcg cgggctggcg ggcctgacgc cttcgttcac cggcgactgg acggttccgg
    10861 acgatccgga cgtcttcgac gccgctgcgc cgggcggggt ctccggccac ggcggcgacg
    10921 cgttcgatct tcaatcggtc ggcctggcgc aggcccgctt cctgcggatc accgacgcga
    10981 atctggcgat cggcccacca ggcgcctcgg aagggtttga cctcgacgcc gtcgtggcgc
    11041 tgcacgcggt cgcgctcgac gccggcccgg acgccgacgg tgacgggctg agcgacgcga
    11101 ccgagacgca ggtctacggc accagcccgt ccgacccgga cagcgacggc gacgggacct
    11161 ccgatggtgt cgaggcggcc acctgccgcg cgccgctcgg ggcctcgacc gcgccgtcct
    11221 tcgctccgtc gctcgatctc gaggtcttcg aggcgcagcc gacgatggtg cgctggaaca
    11281 cgctggggcc gggaaactcc tacgacctgg tgcgcggcag cacggcggcg ctgcacccgg
    11341 tcggcgggat gatcgacctg ggcgtcgtcg cctgcatcga gaacgactcg accgatctgt
    11401 cgaaccgcac tctcggcgac gccgcgatcc cgccggccgg ccaggcgttc ttctatctcg
    11461 cccgcccgac gccgcgcggg acgccgcccg ggtacggcct gtcgtcctcc ggtctgccgc
    11521 gcgtccccgc ctccggggac tgtcagtgat caacgctccg ggcggcgcgg atccgcgccg
    11581 cctccggagc gtcgccgcgg cgcttctcgt cgcggcggcg ttcctgcgcg cttcctccgg
    11641 tgctttcgcc ggcgaagact ccgtcgagga cggggccgcg cccccggcgc ccccggcgga
    11701 ggcggggcct tcgcctgaac cggacgcagg gtccagcgcg ccgtcggtcc ccgaggagac
    11761 gatcacggtc cgcgccccca ccgacgtgct gcgcgccgac cccacagcgt tcggcacggt
    11821 ggtgcggaag gagcagtggg ctgatcggat cacgaccatt cccgatctgc tgcgcgaggt
    11881 ggtcggcgtg caggtgaagt cgctcggcga cagcttctcg accgtgtcga tccgcggctc
    11941 gacggccgag caggtgatgg tctacctcga cggcgtgccg ctcaaccgcg gcgccggggg
    12001 cgccgtcaac ctcgccgaca tcccgctcgc gcaggtggag cggatcgagg tgtaccgcgg
    12061 catgaccccc tcttcactgc cggaggcgtc gatcggcggc gcgattctca tccacacgcg
    12121 cgccccgaag ggccggttcg ctgcggacgg ctacgccggg ctcggctcct acggcagcgg
    12181 ggagttcagc gccgggttcg gcggcgagaa ggggcgcggc ggctggcgga tcgccacgga
    12241 cggcggcacg agcgacggcg acttcccgtt ctgggacaac aacggcactc cggacgatcc
    12301 ggccgacgac ggcgtcgctc cgcgcgtcaa caacgacttc acgagcggcc acgtcctggc
    12361 gcgcggccac ctgcgcgcgg ccgagaccct cttcaccttc gaggcggacg cgttccgccg
    12421 cgagcagggg atccccggcg tcgactcggt gcagtcggag ggggcccgtc tcgagaccgc
    12481 acggctcctt ctggcggcgc gcgccgagcg ggcggggctg ctgtccgggc ggctcgtcct
    12541 ccacggcggc ctgacgcgac acgacgagtc ggaggagttc aacggccaag gcacccactt
    12601 cgtcttcggc gcgaacaccg acaaccgcta catctccacg agcctgagcg gcggcggcac
    12661 gctggtcgcg gggtcacggc aggcgatcgc cttcctcgcg tcgctgcgcg acgagaccgc
    12721 cgacctgcgc gacccctccc ccggcgaggg acaggtgggt ttcgcagaac ggcggatcct
    12781 ggcggcgacg ctcgaggacc agatcgtgct cgcggccggg cgggtgcaga tcgtcccgtc
    12841 gctgcgcttc gaatcttccg ccagcgacta cgcccccgga gaggccggcg ggggcgtgtc
    12901 cggctcgggg gatgtcgact ccgacgaggt gaccggccgg atcggcgcgc gctgggatct
    12961 ctccgccgcc tggtcgattc gcggcaacgc cgggaactac caccggcttc ccgacatcac
    13021 cgagctgtac ggccatcgcg gtggggtcgt cggcaacccg ggcctcctgc cggagcgcgg
    13081 cacgaacgcc gatctggggc tgacctggca ggcggtccag ccgatcggat ggttccggac
    13141 catgcgtgtc gaaggggtcc tgttcggaac cgacgcggaa gacctgatcg tgtacgagcc
    13201 gaccccctcc g
//
LOCUS       DASRIQ010000193         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1387853, whole genome shotgun sequence.
ACCESSION   DASRIQ010000193 DASRIQ010000000
VERSION     DASRIQ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387853"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFD06_04800"
     CDS             complement(<1..513)
                     /locus_tag="VFD06_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02886.1"
                     /translation="MSEPRKSSTSPAAHGADAPRAPLGLRLLASLGRFWPFLLLAAIA
                     FLGWEDLRRLDLAAVRAALRVLDPRWLLIAAGLTVLNLALFGLYDLAALAGSPVPRAA
                     RWRLGALAFAWSNFLTLGPLAGPAIRIWLYAPYNLEAGALRGAVIATMIAFASALGAI
                     LLCVALLPPLP"
     gene            complement(510..1022)
                     /locus_tag="VFD06_04805"
     CDS             complement(510..1022)
                     /locus_tag="VFD06_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZN02887.1"
                     /translation="MTGRRSPSNHRAHQLALRFLAKEWDQADETERRVLLAIAEQLGT
                     HRGTHRKDPRTFGERLADQVATFGGSWTFIIFFLLVLLAWIALNTWALAAQAFDPYPY
                     ILLNLLLSCLAALQAPVILMSQNRQAAWDRQRAEEDYEINLKAELEIKALHDKIDGLA
                     ARIESLRGGR"
     gene            complement(1030..1929)
                     /locus_tag="VFD06_04810"
     CDS             complement(1030..1929)
                     /locus_tag="VFD06_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02888.1"
                     /translation="MRRASAALLIALLLSILGAGRAGAQDPPEDLPEETVTPREFEGP
                     EDPPPAPQETPPPPSRVAAPRPSADRDARRLFQRFAEDAAIIPGGWVEGLLSYEHFDG
                     GVDRAFLVGLLAFQVGEENEAGLRLGFEWLEADAPDPDGTGLADIDVYFKHRFAGASP
                     CAIGGLLKLPTADEDEGLGTGKADFEFFGACRANLEAVSFTANAGARYNGDPDPPFPE
                     SEVSILAGAGAIFPVGREMSIIVETTWESERLDGVGNDARLIIGLQGVATRPGFGFRG
                     ALALPLTDAAPDYQVLFGAVYLY"
     gene            2046..>3391
                     /locus_tag="VFD06_04815"
     CDS             2046..>3391
                     /locus_tag="VFD06_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN02889.1"
                     /translation="MTTTRRRFVLGSMAAAGAGAIAASGASGLVNAAESAAGSTAAPE
                     AAALKPPGRSEALLLSNPEHPKPSTLDRLDQKWHQGRVTRLKQRLNEEGLAGILLADR
                     WSIIYFSGLWFTSTERPFTLFVPAATDTPPTFFHPSLDRDLVGSWWIKDTESYFDFLH
                     GDGAFPNLGKVQMGATVDLNRWLLKGLKKRGHGGKPLGVDFPITAEWTTAVEEILPGT
                     TIRKAGAIGEKMRMVKTAEEIALTQRAMNYWSHIHAFARDYILEHGTNATDFEVQMAA
                     VRYGTDLILRDLKRDGKPHTAVGVEIGIGVRTGIGTAYPHPNQFHHNKIKKGDALQVA
                     GVVTIAGCGGELYRAYQIEPWSDAREKMWQVHTNSCLIQAEESAAGVTCSYVAKRVHD
                     YQVKNGMAKYIYHRPAHGQGWEGHQPPYVALGDHTMLEEGMMFSNEPGLYAPDLGFG"
BASE COUNT          589 a         1166 c         1190 g          446 t
ORIGIN      
        1 gggaagcggc gggaggagcg cgacgcacag gagaatcgcg cccagcgccg aggcgaacgc
       61 gatcatcgtg gcgatgacgg cgccacgcag cgcccccgcc tccaggttgt acggggcgta
      121 gagccagatc cggatcgcgg gcccggccag cggcccgagc gtcaggaaat tgctccaggc
      181 gaacgccagc gccccgaggc gccagcgggc ggcccgaggg accggcgacc cggccagcgc
      241 ggccagatcg tagaggccga acagggccag gttgaggacg gtcaggccgg cggcaatcag
      301 gagccagcgc gggtcgagaa cgcgcaacgc cgcccgcacg gcggcgaggt cgagccgccg
      361 gagatcctcc cagcccagaa acgcgatggc ggcgaggagg aggaacggcc agaaacgccc
      421 gaggctcgcg aggagccgca gcccgagcgg cgcgcgcggc gcgtcggcgc catgcgccgc
      481 cggcgaagta gaggattttc ggggctcgct caccggccgc cgcgcagcga ctcgatcctg
      541 gcggcgaggc catcgatctt gtcgtgcagc gccttgatct ccagctccgc cttcaggttg
      601 atctcgtagt cctcctcggc ccgctgccgg tcccacgccg cctgacggtt ctggctcatc
      661 aagatcaccg gcgcctgcag cgcggcgaga cacgagagga gcaggttcag caggatgtac
      721 ggatacggat cgaaggcctg cgccgcgagg gcccaggtgt tgagggcgat ccaggcgagc
      781 aggaccagga ggaagaagat gatgaaggtc caggagccgc cgaacgtcgc gacctggtcc
      841 gccagccgct ccccgaaggt gcgcgggtcc ttgcggtggg tgccgcgatg cgtgccgagc
      901 tgctcggcga tggcgaggag gacgcgacgt tccgtctcgt cggcctggtc ccactccttg
      961 gccaggaatc gcagcgcaag ctgatgcgcc cggtggttcg agggagaacg gcgcccggtc
     1021 atcggcacct cagtagagat agacggcgcc gaagaggacc tggtagtcgg gcgccgcgtc
     1081 ggtcagcggc agcgccagcg ctccgcggaa cccgaagccg gggcgcgtcg ccaccccctg
     1141 cagaccgatg atcaggcggg cgtcgttgcc gaccccgtcg aggcgctcgc tctcccaggt
     1201 cgtctcgacg atgatcgaca tctcgcggcc gaccgggaag atggcgccgg cacccgcgag
     1261 gatcgagacc tcgctctcgg ggaagggcgg atcgggatct ccgttgtagc gcgcaccggc
     1321 gttcgcggtg aaggagaccg cctccagatt cgcacggcag gcgccgaaga actcgaagtc
     1381 ggccttgccg gtgccgagcc cctcgtcctc atcggcggtc gggagcttga gcaggccgcc
     1441 gatggcgcac ggcgaggcgc ccgcgaagcg gtgcttgaaa tagacgtcga tgtcggcgag
     1501 gccggtgccg tccggatccg gcgcgtcggc ctcgagccac tcgaatccca gccggagccc
     1561 cgcctcgttc tcctccccca cctggaaggc gagcaacccc acgagaaagg cgcgatcgac
     1621 gccgccgtcg aaatgctcgt aggagagcag gccctccacc cagccgccgg ggatgatcgc
     1681 cgcgtcctcg gcgaagcgct ggaagaggcg gcgcgcgtcg cggtccgcgg agggtcgcgg
     1741 cgcagcgact ctcgacggtg gcgggggtgt ttcctgaggt gcggggggcg gatcctcggg
     1801 accctcgaat tcccgcggcg tcaccgtttc ctcgggcaga tcctccggag gatcctgcgc
     1861 gccggcgcgc ccggcgccga ggatcgaaag caacagagcg atcagaagcg cagcgctggc
     1921 tcgtcgcatg gcccccggct tctaccgtac ttccagcgtg ttgacaagcc ctcgcgcact
     1981 ccctcaccat cgccgcttcc gcgccgcccg gttcgggcgc ggcgcgccgg atgacggggg
     2041 gaacgatgac gacgactcga cgcaggttcg tgctcggttc gatggccgcg gcgggggccg
     2101 gcgccatcgc cgccagcggc gcctcaggcc tcgtgaacgc ggccgaatcg gcggccggct
     2161 ccacggcggc gccggaagcg gccgcgctga agcccccggg ccgctccgag gcgctcctcc
     2221 tttccaatcc ggagcatccg aagccgtcga cgctcgaccg gctggaccag aagtggcacc
     2281 aggggcgcgt cacccgcctg aagcagcggc tcaacgaaga ggggctggcc ggcatcctcc
     2341 tcgccgaccg ctggagcatc atctacttca gcggcttgtg gttcacctcg acggagcggc
     2401 cgttcactct cttcgtaccg gcggcgaccg acactccgcc gaccttcttc cacccgagcc
     2461 tcgatcgcga cctcgtcggc tcctggtgga tcaaggacac cgagtcgtac ttcgacttcc
     2521 tgcacggcga cggtgcgttc ccgaacctcg gcaaggtgca gatgggggcg acggtcgatc
     2581 tcaaccgctg gcttctcaaa gggctgaaga agcgcggcca cggcggcaag ccgctcggcg
     2641 tcgatttccc gatcaccgca gagtggacca cggcggtcga ggagattctg cccggcacga
     2701 cgatccggaa ggccggcgcc atcggcgaaa agatgcgcat ggtcaagacg gccgaggaga
     2761 tcgcactgac gcaacgggcg atgaactact ggagccatat ccacgccttc gcgcgcgact
     2821 acattctcga gcacggcacc aatgccaccg acttcgaggt gcagatggcc gcggtccgct
     2881 acggcaccga cctgatcctg cgagacctga agcgcgacgg gaagccgcac acagccgtcg
     2941 gcgtggagat cggcatcgga gtgcgcacgg ggatcggcac cgcctacccg cacccgaacc
     3001 agttccacca caacaagatc aagaaggggg acgccctgca agttgcaggc gtcgtcacca
     3061 tcgccggctg cggcggcgag ttgtaccgcg cctaccagat cgagccgtgg agcgacgcgc
     3121 gagagaagat gtggcaggtc cacacgaatt cctgcctcat ccaggccgag gaatcggccg
     3181 ccggcgtcac ctgctcgtac gtcgcgaagc gggtgcacga ctaccaggtg aagaacggca
     3241 tggccaagta catctaccat cgccccgccc acggccaggg ttgggaaggc caccagcccc
     3301 cgtacgtcgc gctgggcgat cacaccatgc tcgaggaagg gatgatgttc agcaacgagc
     3361 cagggctcta cgcgcccgac ctcgggttcg g
//
LOCUS       DASRIQ010000194         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1136906, whole genome shotgun sequence.
ACCESSION   DASRIQ010000194 DASRIQ010000000
VERSION     DASRIQ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136906"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFD06_04820"
     CDS             complement(<1..282)
                     /locus_tag="VFD06_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HZN02890.1"
                     /translation="MKRTLIAAALLLVGAAPCLASPSLDRLRAERSGGAVVISLQIRE
                     ALDPPRLEEIEAGIESVLRYRIRLVRRRAGLLDEELADVEVEACVRRDAL"
     gene            complement(441..1367)
                     /gene="lpxC"
                     /locus_tag="VFD06_04825"
     CDS             complement(441..1367)
                     /gene="lpxC"
                     /locus_tag="VFD06_04825"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZN02891.1"
                     /translation="MSQQRTVRRAVDIEGVGLHSGADARIRILPAPADHGIVFSRRDL
                     HGASVEATPEAIGDSSLATTISRNGVSIGTVEHLLAALRGLGIDNARIEVDGPEIPIL
                     DGSAVPFVVAIRDAGGSVTQRAARRPLAVRRPLSIRDGERAILALPSREFRISYAIDF
                     PHPAIGYQAVALSVDEEVFAASIAPARTFCLLRDVQAMRAAGRARGGSLDNAVVVGDD
                     GIVSGALRFRDEFVRHKVLDLIGDLALLGAPLQAHVVVFKGGHRLHAQLVRQLRAGAG
                     AGPVARPHSPQWYDRFAHLGEALVPRPSVLTT"
     gene            1690..2688
                     /locus_tag="VFD06_04830"
     CDS             1690..2688
                     /locus_tag="VFD06_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02892.1"
                     /translation="MTRFRSEVEASLAALAAPIDLPASPVLSEDAHAVDAVRNVLEAA
                     SAGGQREILRAVLQGSEICFQRTILFIGRDQTLVGWERRGFGDDRPGRNAGGVPLQIS
                     GGDHLPGRARGGGAMIHAGPEGPGDDVVRALGGDAPVTSAAAPLMVRTRAVAVLYGDA
                     FEPLAAGRLGLFDLLARVGGLALEIRAHRRRLTTETGALLPAAAGVRRERVVAFDANP
                     AAPEEAEMQALLGDIETLPRREEGEGLSPEEQRQHNDARRFASLLVSELLLYNEEAVI
                     QGRKQRDLSQRLAPEIERTRRAYEARIPSALRGGPRYLEDELLRILADGDEALLTL"
     gene            complement(2689..>3862)
                     /locus_tag="VFD06_04835"
     CDS             complement(2689..>3862)
                     /locus_tag="VFD06_04835"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZN02893.1"
                     /translation="VAARERGLPYLSMAETVGRLFLQGHHSMVVSGTHGKTTTSALLA
                     WVLHAAGRRPSFLVGGVLRNFDRSFDLGDGEEFVIEGDEYETAFFDKGPKFLHYRPRT
                     ALLTSVEFDHAEMYRDLEAVKIAFRRFVDLLPPDGLLVVCVDDPNAAAIASGSRAPVQ
                     TYGIGEGAEWWGRAVQAGPDGVEIEIARAGEPFLRTRTPLFGPHNLRNALAVTAAAAS
                     RGVAADDIARGLSSFAGVRRRQEVRGETSGITVLDDFAHHPTAVRLTLEGLRQQYGDR
                     RLWAVFEPRTNTTRRSVFQTEYAEAFDAADRVIVSAVDHPERAPEGERFSAERLVRDL
                     EDRGRWAQYLRTVDAIVDRVAREGRPGDVVAIMSNGAFGGIHAKLLDALQRRPPAD"
BASE COUNT          572 a         1376 c         1390 g          524 t
ORIGIN      
        1 gagcgcgtcg cgccggacgc aggcctcgac ctcgacgtcg gccagctcct cgtccaggag
       61 cccggcccgc cgacgcacca gccggatccg gtaacgcagg acgctctcga tgccggcctc
      121 gatctcctcg aggcgcggcg ggtcgagcgc ctcccggatc tgcagggaga tcaccaccgc
      181 cccccccgaa cgctccgcgc gcaggcggtc gagggagggg gaagccagac agggcgccgc
      241 gccgacgagc aggagcgcgg ccgcgatgag ggttcgtttc actcggagat cggcgatagc
      301 gcccgcgtgg cgggcgccgc cggcagacgg ggagtctagg agcctctccg ggaaatggca
      361 atggccgcgt tcgggacgcc ggggctttgt cccggcgagc ctccccggac cgatcggtcc
      421 ggagtgacct gcgcctcaga tcaggtggtc aggaccgagg ggcgcggcac gagcgcctcg
      481 ccgaggtgcg cgaagcggtc gtaccactgc ggggaatgcg ggcgcgcgac cgggccggcc
      541 cccgcgccgg cgcggagctg ccggaccagt tgggcgtgca gacggtggcc tcccttgaag
      601 accacgacgt gcgcctgcaa cggggccccc agaagcgcga ggtcgccgat caggtcgagc
      661 accttgtgac gcacgaactc gtcgcggaag cggagggcgc cactgacgat gccgtcgtcc
      721 cccaccacca cggcgttgtc cagcgatccg ccgcgggcgc gaccggcggc gcgcatcgcc
      781 tggacgtcgc gcaacagaca gaaggtccgg gccggggcga tggaggcggc gaacacctcc
      841 tcgtcgaccg acagcgccac cgcctggtag ccgatggccg gatgcgggaa atcgatcgcg
      901 taggagatgc ggaactcgcg cgacggaagc gcgagaatgg cgcgttcccc gtcccggatc
      961 gagagcggac gccggacggc gagcgggcgg cgcgccgccc gctgggtgac gctccccccc
     1021 gcgtcgcgga tcgccacgac gaaggggacc gcgctgccgt cgaggatcgg gatctccggc
     1081 ccatcgactt cgatccgcgc gttgtcgatt cccaggccgc gcagcgccgc cagcagatgc
     1141 tcgacggtcc cgatcgagac gccgttccgc gagatggtcg tggcgaggct cgaatcgccg
     1201 atcgcctcgg gggtcgcttc caccgaagcc ccatggagat cgcgccgcga gaacacgatg
     1261 ccgtggtcgg ccggtgcggg caggatccgg atgcgggcgt cggcaccgga atggagcccg
     1321 actccctcga tgtcgacagc ccggcgcacc gttctctgct ggctcatcat gcaagtccct
     1381 cggccccccc aggcgggcgc gctcccttag agcagggccc gtgccagagg cggacactgc
     1441 tcggaataga ccattaacgt cttttgaatc agcgtgatac gcaaccgcac tcgcaacctg
     1501 gtggagcctg cgcggcgtgg cgaatggtat ttctgccacg cttgcctgtg gtatccgcac
     1561 caatcatctg cgccgccatc acggcgtttg acagccgttc gccgccggtg ctactgtcgc
     1621 gcgccgctgg cgatgggcat cttgagcgac gatccggcat ccgaagcgac gcgtcactgg
     1681 gcggagcgcg tgacgcgctt tcgttccgag gttgaggcgt cgctcgccgc gctcgcggcc
     1741 ccgattgatc tccccgcctc cccggtcctt tcggaagatg cgcacgccgt cgacgcggtg
     1801 cggaacgtcc tcgaagcggc gagcgccggg ggccagaggg agatcctgcg ggccgtcctt
     1861 cagggaagcg agatctgctt tcagcggacg attcttttca tcggccgcga ccagacgctg
     1921 gtcggatggg agcggcgggg attcggcgac gaccgtcccg ggcgaaacgc tggaggcgtt
     1981 ccgctgcaga tctccggcgg agatcatctt cccgggcgtg cccgcggcgg gggcgcgatg
     2041 atccacgcgg gtccggaggg acccggcgac gacgtcgtgc gggctctcgg aggcgacgcc
     2101 ccggtcacat cggcggccgc gcctctgatg gtgcggaccc gcgccgtggc cgttctctac
     2161 ggcgatgcct tcgaacccct ggccgccgga cgcctgggac tgttcgatct cctcgcacgg
     2221 gtcggtggcc tggcgctcga aatccgcgcg caccgccgga gactcaccac ggagaccggc
     2281 gcgctgctgc cggcggccgc gggcgtccgg cgcgagcgcg tcgtcgcatt cgacgcgaat
     2341 ccggcggcgc ccgaggaggc ggagatgcag gcgctgctcg gcgacatcga gacgctgccg
     2401 cggcgcgagg agggggaagg cctgtccccc gaggagcagc gccagcacaa cgacgcgcgg
     2461 cggttcgcga gccttctggt gtccgagctc ctgctctaca acgaggaggc ggtcatccag
     2521 gggcgcaagc agcgcgacct ctcgcagcgg ctggcgcccg aaatcgagcg gacgcgacgg
     2581 gcgtacgagg cgcgcatccc ctccgccctc cggggtgggc cgcgctatct cgaggacgag
     2641 ctgctgcgga tcctcgccga cggggacgag gcgcttctca ctctctgatc agtcggccgg
     2701 cggacgccgc tgcagggcgt ccagcagttt cgcgtggatc cccccgaacg cgccgttgga
     2761 catgatcgcc acgacgtcgc ccgggcgccc ctcgcgggcg acgcgatcga cgatcgcgtc
     2821 gaccgtccgc aaatactgcg cccaccggcc gcggtcctcc aggtcccgca ccaggcgctc
     2881 cgccgagaag cgctccccct ccggcgcgcg ctccgggtgg tcgacggcgg agacgatcac
     2941 ccggtcggcc gcgtcgaacg cctccgcgta ctccgtctgg aagacggatc gccgggtcgt
     3001 gttggtgcgc ggctcgaaga ccgcccacag acggcgatcg ccgtactgct ggcgcagccc
     3061 ctcgagcgtc agacggaccg ccgtcggatg atgcgcgaaa tcgtccagca ccgtgatgcc
     3121 cgacgtctcc ccccggacct cctgccgccg tctcacgccg gcgaaggagg acagcccgcg
     3181 ggcgatgtcg tcggctgcaa cgccgcggga cgcggcggcg gccgtcacgg cgagggcgtt
     3241 gcgcaggttg tgcggtccga agagaggggt ccgggtgcgc aggaacggct cccccgcccg
     3301 cgcgatctcg atttccactc cgtcggggcc ggcctgcacg gcgcggcccc accactcggc
     3361 cccttcgccg atcccgtagg tctggaccgg ggcgcggctc cccgaggcga tcgcggccgc
     3421 gttcgggtcg tcgacgcaca cgaccaggag gccgtcgggc ggtaggagat ccacgaaccg
     3481 ccggaaggcg atcttgaccg cctcgagatc gcggtacatc tcggcgtgat cgaactcgac
     3541 cgacgtgagc agggcggtgc gcggccggta gtggaggaac ttcggcccct tgtcgaagaa
     3601 ggccgtctcg tactcgtccc cttcgatgac gaactcctcc ccatctccga ggtcaaacga
     3661 gcggtcgaag ttccgcagca cgcccccgac gaggaacgac ggacggcgtc ccgccgcgtg
     3721 caggacccag gcgagcagcg ccgacgtcgt cgtcttcccg tgcgtgccgg agacgaccat
     3781 ggagtggtgc ccctggagga acaggcggcc gaccgtctcc gccatgctca ggtacgggag
     3841 gccacgctcg cgcgccgcga cg
//
LOCUS       DASRIQ010000195         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2411774, whole genome shotgun sequence.
ACCESSION   DASRIQ010000195 DASRIQ010000000
VERSION     DASRIQ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2411774"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VFD06_04840"
     CDS             complement(<1..1042)
                     /locus_tag="VFD06_04840"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HZN02894.1"
                     /translation="MKARTIHEGKAGLGAPLTNTFHKHLDALRKSDSKNPEVQNRYDQ
                     AVKRDRARTGPDISFSGNEADPLFYNTGSGFVEIGTTLGLGRVEDGRGMVLVDVDGDG
                     AQDVVVHNYFRNPIVALHNRAAGEGRWVRLRLRGRTSNRFGIGAKVTVNGRLQELACG
                     AGYLSGNAPELHFGLGPAAHADVAVRWPSGKQDEYKALPVNRIHTMVEGEAAALRSEE
                     LVRHPIAPAVPDPAPPELDVRALMKGLRTLEGEPAAVDGTAVVVFFSVSCHACIQDLQ
                     QMVEHEQRARERGLRLVWVTIDRDLHAVGEEFRVNKAPSLPLRPTGSVAGLATPTVYR
                     ITPDRVEKFTGRF"
     gene            complement(1061..2794)
                     /locus_tag="VFD06_04845"
     CDS             complement(1061..2794)
                     /locus_tag="VFD06_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN02895.1"
                     /translation="MGTRVRALLIAAVGFLVLIALILRARSGQAPVPPPASLDQDYSS
                     ADEDAVIEIEEMMIDVAAQLTRKAWGGVLHHVADDFEGTPLFRTADGPSKVVAGVTLR
                     SAGRDSRAVNRAGFREALDGVDVDDVVFKFPAATLAGETLTGRMKMDAKRERGTSASR
                     WVVQGDVEFQRRGGRWLLRRFQGIEVKTEEGARRFPDVTVPLGLPIAPGDDDRDRTSI
                     TFGRLFLGGIAAGDFDGDGLVDLFVPQTGQDLLFRNTGGKFVECARDRGIIEADAGAG
                     ALFLDYDNDGLLDLLVTNYEPEALRDLKSNALRDNSGHRALALYRNTGGRFVDVTAKA
                     GLTCRGPAMSPCAADVNGDGLLDLYVCMYKDDSVEDPRFSEEVPSVVWAARDGVANQL
                     WINQGDGSFKEEAAARGVADTGWGLAASFCDYDADGKADLYLANDYGENRLFRNRGDG
                     RFEDVTAKSATGDAGFGMGVTWLDYDADGRMDLYVSNMYSTAGNRILARGPGRMSREQ
                     HSRLLKMASGNSLFRNRGDGTFEDVTAGMGVGRAGWAWSSAAYDFENSGVPHLYVANG
                     FRTSSFATTDI"
BASE COUNT          461 a         1100 c         1048 g          467 t
ORIGIN      
        1 cgaagcggcc ggtgaacttc tcgacgcggt ccggcgtgat ccggtagacc gtcggcgtgg
       61 ccagccccgc aaccgaaccg gtcggacgca gcggcagcga cggcgccttg ttcacgcgga
      121 attcctcgcc gaccgcatgg aggtcccggt cgatcgtcac ccacacgagc cggaggcccc
      181 gctcccgggc gcgctgttcg tgctcgacca tctgctggag atcctggatg caggcatggc
      241 aggagacgct gaagaagacg acgacggccg ttccatcgac ggctgcgggt tcgccctcca
      301 gcgtccggag gcctttcatc agcgcccgca cgtcgagctc cggcggcgcc ggatccggaa
      361 ccgcgggcgc gatcggatgc cggaccagtt cctccgaccg cagcgccgcc gcctcccctt
      421 ccaccatcgt gtggatgcgg ttgacgggaa gggccttgta ctcgtcctgc ttccccgacg
      481 gccagcggac ggcgacatcc gcgtgggccg ccgggccgag gccgaagtgg agttcgggcg
      541 cgtttcccga aaggtagccg gcgccgcagg ccagttcctg gaggcggccg ttcaccgtca
      601 ccttcgcgcc gatgccgaag cggttcgagg tccgcccccg cagccggagc cggacccagc
      661 ggccctcccc cgccgcgcgg ttgtgcagcg cgacgatcgg attccggaag tagttgtgga
      721 cgaccacgtc ctgcgcgccg tcgccgtcga cgtccaccag aaccatgccc cgcccgtcct
      781 cgacccggcc caggcccagc gtcgtgccga tttccacgaa cccggagccc gtgttgtaga
      841 acagcggatc cgcctcgttt ccgctgaagg aaatgtcggg gcccgtccgc gcccggtcgc
      901 gcttcaccgc ctggtcgtag cggttctgca cctcggggtt tttggagtcc gacttccgga
      961 gcgcgtcgag gtgcttgtgg aaggtgttcg tcagcggagc gccgagcccg gcctttccct
     1021 catggatcgt ccgcgccttc acgaggcgcc agaagggcgc tcatatgtcg gtcgtcgcga
     1081 acgacgacgt gcggaagccg ttggcgacgt agagatgggg aacgccgctg ttctcgaagt
     1141 cgtaggcggc cgagctccag gcccagcccg cccggcccac gcccatgccg gccgtcacgt
     1201 cctcgaaggt gccgtcgccc cggttccgga agagcgaatt cccggacgcc atcttgagca
     1261 gccgcgaatg ctgctcccgg ctcatccggc ccggcccccg cgcgaggatc cggttccccg
     1321 ccgtggagta catgttcgag acgtagagat ccatccggcc gtcggcgtcg tagtcgagcc
     1381 aggtgacgcc catgccgaag ccggcgtccc cggtcgcgga cttcgccgtg acgtcctcga
     1441 agcggccgtc cccgcgattg cggaagaggc ggttctcgcc gtagtcgttc gcaagataga
     1501 ggtcggcctt gccgtccgcg tcgtagtcgc agaacgaggc cgcgaggccc caccccgtgt
     1561 ccgccacgcc ccgcgcggcg gcctcctcct tgaagctccc gtcgccctgg ttgatccaga
     1621 gctggttcgc gacgccgtcc cgcgccgccc agacgaccga cggcacctcc tcggagaacc
     1681 gcggatcctc gaccgagtcg tccttgtaca tgcagacgta gaggtccagg agaccgtcgc
     1741 cgttcacgtc cgcggcgcag ggggacatgg cggggccgcg gcaggtcagg ccggccttcg
     1801 cggtgacgtc gacgaaccgc cccccggtgt tccggtagag cgcgagggcg cggtggccgg
     1861 agttatctcg cagagcgttc gacttcaggt cccgcaacgc ctccggctcg tagttcgtca
     1921 ccagcaggtc gagaaggccg tcgttgtcgt agtcgaggaa gagcgcgccg gcgcccgcgt
     1981 cggcttcgat gatcccccgg tcgcgcgcgc attcgacgaa tttaccgccc gtatttcgga
     2041 agagaaggtc ctggcccgtc tggggcacga agaggtcgac cagaccgtcg ccgtcgaagt
     2101 ctcccgcggc gatcccgccc aggaacagcc ggccgaacgt gatcgaggtc cggtcgcggt
     2161 cgtcgtcccc cggcgcgatc ggaaggccga gcggaaccgt gacgtccggg aatcggcgcg
     2221 cgccttcctc ggtcttgacc tcgatcccct ggaaccggcg gagcagccac cggccgccgc
     2281 ggcgctggaa ctccacatcg ccctggacga cccagcgcga cgccgacgtt ccccgctcgc
     2341 gcttcgcgtc catcttcatc cggcccgtga gcgtctcccc cgccagggtc gccgcgggaa
     2401 acttgaagac cacgtcgtcc acgtcgaccc cgtcgagcgc ctcgcggaag ccggcgcggt
     2461 tgacggcccg cgagtcgcgc ccggcggacc gcagcgtcac gccggccacg accttcgagg
     2521 gaccgtccgc ggtccggaac aggggcgtgc cctcgaaatc gtccgcgacg tggtgcagga
     2581 cgccgcccca cgccttgcgg gtgagctgcg cggcgacgtc gatcatcatc tcctcgatct
     2641 cgatcaccgc gtcttcgtcg gcgctggaat agtcctggtc gagagaggcc ggaggcggga
     2701 ccggggcttg cccggagcgc gcccgaagga tcaacgcgat cagaacgagg aagccgacgg
     2761 cggcgatcag gagcgcccgg actcgggttc ccatcggttc aactctagca gcacaggccg
     2821 tcgctgtcac gcgatcgccg ggcgtggtat aacgggcctg tgatgcatcg ggtgctcgcc
     2881 ggaatcacgg ccctcctcgt tctgaccgga accgggtgcg gccgaaagga ggagagtccg
     2941 gtccggaagc ccgcgccgac cggccacgcg agaatgatcg ccctcctccg ggagatcggg
     3001 gaccgcgtcg acgaggatca ctactggatc ggggatcggg aagcgcggga gttgaagcag
     3061 cggctggccg ccctga
//
LOCUS       DASRIQ010000196         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1388659, whole genome shotgun sequence.
ACCESSION   DASRIQ010000196 DASRIQ010000000
VERSION     DASRIQ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388659"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..321)
                     /locus_tag="VFD06_04850"
                     /pseudo
     CDS             complement(124..321)
                     /locus_tag="VFD06_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
     gene            complement(371..496)
                     /locus_tag="VFD06_04855"
     CDS             complement(371..496)
                     /locus_tag="VFD06_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02896.1"
                     /translation="MLSRPLGGARALAPGALPMLDPEGRFVTGSLFGVMRIELAR"
     gene            complement(846..1850)
                     /locus_tag="VFD06_04860"
     CDS             complement(846..1850)
                     /locus_tag="VFD06_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN02897.1"
                     /translation="MFQVEPVLWLQSFASPLTIGFMSAITWLGTVPCLVAIVFVLAFG
                     VRFRVGLAVLFLLCANSLATDVMKDGFALPRPGDVDTRVQQLGGDSAHPIAERGGAGG
                     FFAPLPEGTLAAARGTEGLSYAFPSGHLSSATTLMLSIALLLRSRRVLWLAALWLPLT
                     GLSRLFLGRHYLADLFGGAALGAAVALLGALFFGELALGLERGAAAPWRRAAIVSVAA
                     AALLLAVLVPGLDVATVGRLVAAAILLYIASGLAFREEVEPIARIARTAIAVALCFAA
                     APALAAAFGAAGLAGTPRAEMLQGAILVLVGFGGGAIASRVVPFAGGGRLTRQPVGEA
                     "
     gene            complement(1927..3009)
                     /locus_tag="VFD06_04865"
     CDS             complement(1927..3009)
                     /locus_tag="VFD06_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZN02898.1"
                     /translation="MTGDDAADLEALRERIRRLDADLVAKTAERIALGREIGAIKHRQ
                     GLGAADYAQESIVLQRARDAAAAHAVDPALAEEIVARLIRASLLAQEEDRLKLTALGA
                     GRRAVVVGGAGRMGRWMRRFLSAQGYAAAPLDPQADPADDAAGRRDVATADLVVCAAP
                     PAAIASLYLDWAAAPPQGIVVDIASIKTPLVEPITRLRRAGGRVASIHPMFGPATLLL
                     RDADVVVCDTGDDAAREAIERLFEPTTARLVRLPLEDHDRVMADLLSLAHAAAIAFAL
                     ALPEEGHQVRSTTFQALERLAAAVVRESPEVYYEIQARNPHSAASIARLRDALDRIVA
                     ASAAGDPSPFRALFEAGRARTDPKSS"
     gene            complement(3023..>4289)
                     /locus_tag="VFD06_04870"
     CDS             complement(3023..>4289)
                     /locus_tag="VFD06_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN02899.1"
                     /translation="QSKQGRLINISLTTSPVRDGTGRVVGASKVARDITARVKAEEAL
                     READRRKDEFLATLAHELRNPLAPVRNAVEVLRLKGSPTPEVQWAREVIDRQMRQMSR
                     IIDDLLDVSRITRDVLELRRERVDLATVLNAAIETSQGLIETSGHELDVHLPAHPVFL
                     HADVTRLAQVFSNLLSNAAKFSERGSRITILAERRGDEVVVAVRDTGIGIPGEMQPRI
                     FDIFMQVDRSLERSQGGLGIGLTLVKRLVEMHGGSVEVRSEGRGTGSEFIVRLPIALA
                     DAAAASGPALRPRTARSAPARGRVLVADDNRDAAASLSTMLDILGYETRTASDGAEAL
                     QAAKEFRPEVALLDIGMPKANGYDVARRIRQQDWGKDVVLMAITGWGQIEDRQRTFEA
                     GFDHHLVKPVDPDLLASLLASLHAKRHEV"
BASE COUNT          648 a         1554 c         1560 g          527 t
ORIGIN      
        1 aggatgaagt gaagcaggag catcaccacg agggccagga ggaagtagac aggaggatac
       61 agccgccgcg tcgggaacgg gtgggcggag ccgcttgtgc tctcgccccg atcgctcacc
      121 ccttcacccc tctcgcccac cccatgaccc cttgaagact cgatagaccg cgccgcacag
      181 gcacaaggcg gcccgcaggt cattcaggcg cagcggctcc ttctggtgcc cgatccggtt
      241 ggccgggacc tgcgccaggt actcgaagag cgcgatcccc cacgacacga tcgccgcgac
      301 gtaccacgcg ctcgacttga gccagcagga cgaccttggc gatcaccggc atctgtttcc
      361 ccccctggtt ttatcgcgcc agctcgatgc gcatcacgcc gaagagcgaa ccggtgacga
      421 agcgtccttc gggatcgagc atcggcagag cccccggcgc cagcgcgcgc gcaccgccga
      481 gcggccgcga cagcacggac gtgccgtccc cccagtcgat cccttcgagc gtcgagcgtc
      541 cgccgcggaa ggcgacgaag tagaccgccc ggccatcggg cgtgaccagg ggcgttcccg
      601 atacggcgcc gccgcccgcc caggtcctac tgggcgcccg cgtccgggga tcccagaccc
      661 acttcgtgag cccccccggt gccggctcca gcgggagcgg ggcgacgtgt ggcccgcccc
      721 gacttcctgg gagaggagtt cacgcgccgt cggcggcgcc tcaggcttcg gggcgacgac
      781 gttcgccccg catcccagaa gtagcacgga cgccggaagg aggagcctcg ccaccttcgc
      841 ccgggtcaag cctcgccgac cggctgccgg gtgaggcggc cgccccccgc gaaggggacg
      901 acgcgcgacg cgatcgcccc gccgccgaag ccgacgagca ccaggatcgc cccctgcagc
      961 atctccgcgc gcggtgtccc ggccaggccg gccgccccga acgcggccgc gagcgctggc
     1021 gccgcggcga agcacagcgc cacggcgatc gccgtgcgcg cgatcctggc gatcggctcg
     1081 acctcctcgc ggaaggcgag accggacgcg atgtaaagga ggatcgccgc ggccaccagc
     1141 cggccgaccg tcgccacgtc gagccccggc acgaggaccg cgagcaggag cgccgcggcc
     1201 gcgaccgaca cgatcgcggc gcgccgccac ggcgccgccg ccccccgttc gagcccgagc
     1261 gccaactcac caaagaaaag ggcgccgaga agcgcgacgg ctgctccgag cgccgccccg
     1321 ccgaacaggt ccgcgaggta gtgacgcccg aggaagagcc gcgacagccc ggtcaacgga
     1381 agccagagcg ccgcgagcca caggacccgg cgggagcgca ggaggagggc gatcgacagc
     1441 atgagcgtcg tggcggagga gaggtggccg gacgggaagg cgtacgacag cccctccgtg
     1501 ccccgcgccg cggcgagcgt cccttccgga aggggagcga agaagccgcc ggcgccaccc
     1561 cgctcggcga tcgggtgcgc ggagtcgccg ccgagctgct gcacccgcgt gtccacgtcc
     1621 ccgggccgcg gcagggcgaa tccgtccttc atgacatcgg tcgcgaggct gttggcgcag
     1681 agcaggaaga ggaccgccag gccgacgcgg aagcggaccc cgaacgccag gacgaagacg
     1741 atcgccacca ggcacggcac cgtgccgagc caggtgatcg cggacatgaa accgatcgtc
     1801 aggggggagg cgaacgactg cagccacagc accggctcga cctggaacac cgcgtcctcc
     1861 acaacctgga tgagacggac ggcattctaa gcgagaagtg cggggccgcg ggacggcccc
     1921 gaggagtcag ctcgacttcg gatcggtccg ggcccggccg gcctcgaaga gcgcgcggaa
     1981 gggggaaggg tccccggcgg ccgacgcggc gacgatccgg tcgagcgcgt cccggagccg
     2041 cgcgatcgag gcggcggagt gggggttgcg cgcctggatc tcgtagtaca cctccgggct
     2101 ctcgcgtacg accgcggcgg cgaggcgctc gagcgcctgg aaggtggtgc tacgcacctg
     2161 gtgcccctcc tccggcagcg ccaaggcgaa ggcgatcgcc gcggcgtgcg ccaggctcaa
     2221 aaggtcggcc atgacccggt cgtggtcctc gagcggcagg cggacgaggc gggccgtggt
     2281 cggctcgaag aggcgctcga tcgcttcgcg ggccgcgtcg tcgccggtgt cgcagacgac
     2341 cacgtcggcg tcccgcagga ggagcgtcgc ggggccgaac atcgggtgga tcgacgcgac
     2401 ccggcccccc gcccgccgca gccgggtgat cggctcgacg agcggcgtct tgatgctggc
     2461 gatgtcgacg acgatccctt gcggcggggc ggcggcccag tcgaggtaca gcgacgcaat
     2521 ggcggcggga ggggcggcgc agacgacgag gtcggcggtc gcgacgtccc ggcgtccggc
     2581 cgcgtcgtcg gccgggtcgg cctgtgggtc gagcggcgcg gcggcgtaac cctgcgccga
     2641 caggaagcgg cgcatccagc gtcccatccg gccggcgccg ccgacgacca ccgctcgccg
     2701 gccggcgccg agggcggtca gcttgaggcg gtcctcctcc tgcgccagga gcgaggcgcg
     2761 gatgagccgc gcgacgatct cctccgccag ggccggatcg acggcgtgcg ccgcggccgc
     2821 gtcccgcgcg cgctgcagca cgatcgattc ctgcgcgtag tcggcggccc cgagcccctg
     2881 ccgatgcttg atcgcgccga tctcccggcc gagcgcgatc ctttcggcgg tcttggccac
     2941 gaggtcggcg tcgagccgac ggatgcgctc ccgcagcgcc tcgagatcgg cggcgtcatc
     3001 gcccgtcatc ctgttcaggc tctcacacct cgtgccgctt cgcatgcagc gaggcgagca
     3061 ggctcgcgag caggtcggga tcgaccggct tgaccaggtg gtgatcgaaa ccggcctcga
     3121 aggtgcgctg ccggtcctcg atctgccccc agccggtgat cgccatcagg acgacgtcct
     3181 tcccccagtc ctgttgccgg atccggcgcg ccacgtcgta gccgttggcc ttgggcatgc
     3241 cgatatcgag aagcgccacc tccggccgga actccttcgc cgcctggagc gcctcggcgc
     3301 cgtcgctggc ggtgcgggtc tcgtaaccga ggatgtcgag catggtggag aggctcgccg
     3361 ccgcgtcccg attgtcgtcc gcgaccagca cccgaccgcg cgccggcgcc gacctcgcgg
     3421 tgcgcgggcg cagcgcgggg ccggacgccg cggcggcgtc agcgagggcg atcggcagcc
     3481 ggacgatgaa ctcgctcccg gtgccgcgcc cctcgctccg gacctcgacg ctcccgccgt
     3541 gcatctcgac gaggcgcttg acgagcgtca ggccgatgcc gagccctccc tgggagcgct
     3601 cgagggagcg gtccacctgc atgaagatgt cgaagatgcg cggctgcatc tccccgggga
     3661 tgccgatccc ggtgtcgcgc accgccacca ccacctcgtc accgcgccgc tccgccagga
     3721 tggtgatccg gctgccgcgt tcggagaact tggcggcgtt gctcaggagg ttcgagaaga
     3781 cctgcgccag ccgggtgacg tcggcgtgca ggaagaccgg gtgcgcgggg aggtggacgt
     3841 ccagctcgtg cccggaggtc tcgatgagcc cctggctcgt ctcgatcgca gcgttcagga
     3901 ccgtggcgag atcgacccgc tcgcggcgca gctcgagaac gtcgcgggtg atgcgcgaga
     3961 cgtccaggag gtcgtcgatg atccgggaca tctggcgcat ctgtcgatcg atcacctcgc
     4021 gcgcccactg cacctcgggg gtgggggagc ccttgaggcg cagcacctcg acggcgttgc
     4081 gcaccggcgc gaggggattg cgcagctcgt gggccagcgt cgccaggaac tcgtccttgc
     4141 gccggtccgc ctccctcaga gcctcctcgg ccttgacccg ggcggtgatg tcgcgcgcga
     4201 ccttggacgc gccgacgacc cgacccgtgc cgtcccggac cggcgaggtc gtcagggaga
     4261 tgttgatcag gcggccttgc ttggattgg
//
LOCUS       DASRIQ010000197         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2703008, whole genome shotgun sequence.
ACCESSION   DASRIQ010000197 DASRIQ010000000
VERSION     DASRIQ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703008"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFD06_04875"
     CDS             complement(<1..1034)
                     /locus_tag="VFD06_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HZN02900.1"
                     /translation="MSAPLEFQSHPGDLAALSADLGIVYRLKGDGGIAGPVGDALSQR
                     LLEVAREDRFNGRAGRLLLWHAPDGAFPCRRYLLLGLGSRDEVTLERYRRVLGDAFVE
                     ADRLGAASVAVPLLVDDRLGFSPNEAARALAEAALLGTYRFDRYRSEPRPGRRHLKAI
                     RVALGPAPAKGVSAGIAWGERASSAVNLARDLVNEPPGILTPPRLAEIAEEQAKAAGL
                     EVEVFGPDALRKMGMGGILGVAQGSHKEPRLVRLQYRPKGKADRKVAVIGKGLTFDSG
                     GLSLKTSEGMETMKCDMAGSAAVLATLTALGDLGCRSEVTGLMGLAENMPGGEAIRPG
                     DVLRIMNGKTV"
     gene            complement(1068..3041)
                     /gene="dxs"
                     /locus_tag="VFD06_04880"
     CDS             complement(1068..3041)
                     /gene="dxs"
                     /locus_tag="VFD06_04880"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZN02901.1"
                     /translation="MSEALSGNLPLPLARGAERTEPRAEPSATAAPLLARIQSPADIK
                     GLDATQLKQLAEELRQYIIDVVSRTGGHLAPSLGVVELTIALHAVYDAPRDRIVWDVG
                     HQAYGHKVLTGRRDRLPSLRQYQGLSGFVSPAESPYDAFGVAHASTSIAAAMGMVVAR
                     DMAGEDYHVLTVTGDGAMTGGLAYEGLNNAGHSERDLLVILNDNEMSISPNVGAIARH
                     LTDITSSGAYNRMREEIRSLAKRLPLGEPAGELAKRVERGLKDFFVPGGLFQALGFKY
                     YGPIDGHDLDELVSVLNNLKKVKGPRLLHVITKKGKGYSFAEADSCVYHGVTAFDPAS
                     GQMAAAGKGAAPDYNKVLCDTLSRLAAEDRRVVAVTAAMADGTGLVKFKERHPDRFHD
                     VGIAEAYGVTFAGGLAISGMRPVAAIYSTFLQRAYDQIIHDVGVQRLPVVFCLDRAGL
                     VGADGPTHHGVFDLSYLRAVPNFIIAAPKDGDELADLLKTAVDQTERPFAIRYPKRTS
                     RRYQPDRVPQVLPIGSWEALSEGRDACLLAVGSMVETAEAVRDLLRVDGIDVGVVNAR
                     FVKPLDETMLRDLASRLPVLVTLEENALAGGFGAAVHESLAASGHSAPALHHVGLPDR
                     FVTHGSVGQLLEEVGLSPARVAERLRPLLRGRA"
BASE COUNT          451 a         1133 c         1109 g          451 t
ORIGIN      
        1 accgtcttgc cgttcatgat ccgcaggacg tcgccggggc ggatcgcctc cccgcccggc
       61 atgttctccg ccagccccat caggccggtg acctccgacc ggcagccgag atcgccgagc
      121 gcggtcaggg tcgcgagcac cgccgccgat ccggccatgt cgcacttcat cgtctccatc
      181 ccctcgctcg tcttgagcga caggccgccg gagtcgaagg tcagcccctt gccgatcacc
      241 gcaactttcc tgtcggcctt ccctttcggc cggtactgca ggcggaccag gcgcggctcc
      301 ttgtgcgagc cctgcgccac gccgaggatc cctcccatcc ccatcttccg gagcgcgtcg
      361 gggccgaaca cctcgacctc caggccggcc gccttcgcct gctcctcggc gatctccgcg
      421 aggcgcggcg gcgtcaggat cccgggcggc tcgttgacca ggtcgcgcgc caggttgacc
      481 gccgaggacg cccgctcccc ccaggcgatt ccggctgaga cacccttcgc cggggccggg
      541 ccgagcgcga cccggatcgc cttcaggtga cgccgcccgg ggcgcggctc ggagcggtag
      601 cgatcgaagc gataggtccc gagcagggcg gcctccgcca gcgcccgcgc cgcctcgttc
      661 gggctgaagc cgagccggtc gtcgacgagc agcggcaccg cgacggacgc cgcgccgagc
      721 cggtccgcct ccacgaaggc gtcgcccagt acgcggcggt accgctcgag ggtcacctcg
      781 tcgcgcgacc cgagcccgag caggagatag cggcggcacg gaaaggcgcc atcgggggcg
      841 tgccacagaa gcaggcggcc ggcgcggccg ttgaagcgat cctcgcgggc cacctcgagc
      901 aggcgctgcg acagcgcgtc gccgaccgga cccgcgatcc cgccgtcccc tttcagccgg
      961 taaacgatgc cgagatcggc cgaaagagcg gcgagatccc cgggatggct ctggaactcg
     1021 agaggggcgc tcaagacccg cgcgctccta ggtccgcgcg gccgtcatca ggcgcggccc
     1081 cggagcaggg gtctcagccg ctcggcgacg cgggccggtg agagaccgac ctcttcgagg
     1141 agctgtccga ccgagccgtg cgtgacgaac ctatccggaa ggcccacgtg gtgcagcgcc
     1201 ggcgccgagt ggcccgacgc ggcgaggctc tcgtgcacgg cggcgccgaa cccgccggcg
     1261 agtgcgtttt cctcgagcgt caccagcacc ggcaggcgcg acgccaggtc gcggagcatc
     1321 gtctcgtcga gcggcttgac gaaccgcgcg ttcacgacgc cgacatcgat cccgtcgacg
     1381 cgcaggaggt cccggaccgc ttcggccgtc tccaccatcg acccgaccgc cagcaggcac
     1441 gcgtcgcgcc cctcggagag cgcctcccat gagccgatcg gaaggacctg ggggacccgg
     1501 tcgggttggt agcgccggct ggtccgcttc gggtagcgga tggcgaaggg gcgctccgtc
     1561 tgatcgaccg cggtcttcag gaggtcggcc agctcgtccc cgtccttcgg ggcggcgatg
     1621 atgaagttcg gcaccgcccg gaggtacgac aggtcgaaga cgccgtggtg cgtcggaccg
     1681 tcggcgccga cgagcccggc gcggtcgagg cagaacacca ccggcagccg ctgcaccccc
     1741 acgtcgtgga tgatctggtc gtaggcgcgc tggaggaacg tggagtagat cgccgccacc
     1801 ggccgcatgc cggaaatggc cagcccgccg gcgaacgtca caccgtacgc ctcggcgatg
     1861 ccgacgtcgt ggaagcggtc ggggtggcgc tccttgaact tcacgagccc ggtgccgtcg
     1921 gccatcgcgg cggtcaccgc cacgacgcgg cgatcctccg ccgccaggcg cgacagggtg
     1981 tcgcacagga ccttgttgta gtccggggcg gcccccttgc cggccgccgc catctgaccc
     2041 gacgcgggat cgaaggcggt gacgccgtgg tagacgcacg aatcggcctc ggcgaaggag
     2101 taccccttcc ccttcttcgt gatcacgtgc aggagccgcg gccccttcac cttcttgagg
     2161 ttgttcagga cgctgaccag ctcgtcgagg tcgtgcccgt cgatcgggcc gtagtacttg
     2221 aacccgagcg cctggaagag gcctccgggg acgaagaagt ccttgagccc gcgttccacg
     2281 cgcttggcca attcaccggc cggctcgccg agaggcagcc gcttcgcgag cgaccggatc
     2341 tcctcgcgca tccggttgta cgccccgctc gaggtgatgt cggtcaggtg ccgcgcgatc
     2401 gcgccgacgt tcggcgaaat cgacatctcg ttgtcgttca ggatcaccag caggtcgcgc
     2461 tcggaatggc cggcgttgtt gagcccttcg taggcgagcc cgccggtcat ggcgccgtcc
     2521 ccggtcaccg tcaggacgtg gtagtcctcc cccgccatgt cgcgcgccac gaccatcccc
     2581 atggcggcgg cgatcgaggt gctggcgtgc gccacaccga aggcgtcgta gggggactcc
     2641 gccggggaga cgaagccgga gagcccctgg tactggcgca gcgacggcag gcggtcgcga
     2701 cggccggtga gcaccttgtg gccgtacgcc tggtgcccga cgtcccagac gatgcggtcg
     2761 cgtggcgcgt cgtagacggc gtgcagcgcg atggtcagct cgaccacgcc caggctgggc
     2821 gcgaggtgcc cgccggtgcg ggacacgacg tcgatgatgt actggcgcag ctcctcggcc
     2881 agttgcttga gctgggtggc gtcgagcccc ttgatgtcgg ccggggactg gatgcgcgcc
     2941 aggagcggcg cggcggtggc cgaaggctcc gcccgaggct cggtcctctc ggcgccgcgc
     3001 gccagaggga gtggcaggtt cccgctaagt gcctcggaca tgacggccgg tcatgttatc
     3061 ggcgtcacct ccgggggtca agcacatcgt gcgaggcgtg ctattctccc gcgccgagcc
     3121 cgcggtgatc cccttcaagg acga
//
LOCUS       DASRIQ010000198         7994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1894267, whole genome shotgun sequence.
ACCESSION   DASRIQ010000198 DASRIQ010000000
VERSION     DASRIQ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894267"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFD06_04885"
     CDS             <1..645
                     /locus_tag="VFD06_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HZN02902.1"
                     /translation="KEKKITLRGAKVAVQGFGNAGSIAARLLHEDGATIIAVSDSSGG
                     IHSPKGLDIAAVEAHKGRTGSLKGYAEADAISNERLLELECDILVPAALENQITTENA
                     ARVRAKIVAEAANGPTTPGADDILSRNGVFLIPDILANAGGVTVSYFEWVQSLQSFFW
                     EEQQVNEHLEKIMTRSFHEVLALAKKHQVHMRTAAYILGVGRVAEATRIRGIYP"
     gene            645..1463
                     /locus_tag="VFD06_04890"
     CDS             645..1463
                     /locus_tag="VFD06_04890"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HZN02903.1"
                     /translation="MRALLATAAVAALLVVAAAPPARAADPPIRPKVVSLRLKMGLMG
                     QITLYAPAMSRPDLLPVLFFAGDWGWRPLQQESASHLASEGRFVVGIDSTEYFKRRLE
                     AADWAADLKQLRDFTNEKAGRPAGGPVLLVGYCWGGEVIPYMLNRGGASGFAGAVLLG
                     PDKDSAFIYRVSLQMTNVPSPPDEAFNVAEELKRLPAGFPVAFIEGELDTNSAARTLE
                     ALVPGPHKRAVVPGGDRQFREVRNIYLGLLSQALAWIEGQGKGAPPTDPPAGGR"
     gene            complement(1471..2190)
                     /locus_tag="VFD06_04895"
     CDS             complement(1471..2190)
                     /locus_tag="VFD06_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN02904.1"
                     /translation="MTGTPTDASPECGHPANELLDFHVNGSLEGDEAAMVAAHVAACA
                     ACARDVRELQSLSEALEAHGVAPASAWRGGRLLWGLGTAAAVLGGIIIVRLGPSESRV
                     ASVPPGNGTPAAVDSAGAAPSGVEKMLDLGGGPMRDAGAGLPRVELEPGVSVLRLTLT
                     HPVPGTELRFGVRGPGDEETVADQALPPHDAMGRVEIPVPASRFATSGTYQVVLKPAT
                     PGGEAGPYYYSFEVRISSDRS"
     gene            complement(2187..2783)
                     /locus_tag="VFD06_04900"
     CDS             complement(2187..2783)
                     /locus_tag="VFD06_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN02905.1"
                     /translation="MDEVLDEKVAVPVRSPSEDDRRLIRRLLVGHVQSIATLDSWIQT
                     VLRTEFHSLHREWDDLRQEVRMRVFRNLRSDRFHGASELRTYVHRIARNTCIDAWRRA
                     GARRDPSTDPALDRRREITSDETTDRLISRDLLRKILLGSTPEERHLLHMVHAEHLSY
                     AEIARKLGVAEGTIKARVFRSRDRLLALRRRLLRIADR"
     gene            3011..6259
                     /locus_tag="VFD06_04905"
     CDS             3011..6259
                     /locus_tag="VFD06_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HZN02906.1"
                     /translation="MRGRSFRVSLRLLAPVLVCLVAGGSIASPPGTEAEPADAAALRP
                     LIHDARWAEAETAARALVAQRAAAYGEDSPEYAAALELLLEVLVRSGRWSEPKTRELA
                     ARAVAINEKTLPPDAPALATSLIRLGGIERLVGPFDHARAILERALAIREAAFGANSL
                     PVAEALAQLGWSMIDTPDPQQAIAMHERALAIRESILGKDDLLVAVSLAGLGGVARNA
                     GDFQKAEAAHERALDIRRRHLRADHPDIALSILSVADAKHRLGDLGGALPLYEEALTL
                     REHEPTPDLPRLSEALLNLGDLLTDLGSLDRARPMYERGVRIRDETIGDGSFITGIGH
                     QSYALFLEAAGDAAGARREFERAIAIFDGATEPGHPLRAVGRQDLGLMLKRQGDLDAA
                     REMLQASLDIWRMAPAGRPGHPFSAVPMIALGSILRDRGDVAAAGPLLDQAVAILEAS
                     YGPAHPRYAEALLERSRLAFREGNDDAALEDALRAEESLRINLRATTRALSESEALRY
                     QEVMISGLDTAFSILLDAGDPPDASTHARTVFEALGRSRTLVLDEIAARHRSITRSDD
                     PKIRSLDEALVAALRNYARLVVEGPSQRSPAGYAPRLQGAREAMERAERGLAAASRAF
                     ADARERQNMTYAAIAKTLPEASALVSFVAYTRSDRAAGKGKPSYLACVVAPGAAAPAV
                     VPLGAAAGIDAAIDRWRKESSTPPAGAAAEESYRLAAAAVREALWDPLAGRLGSARRV
                     FIVPDAAIHALSFATLVDRGGKYLMETGPEFHYVSSERDLLEGAGGPVRGSGLLALGG
                     PDFDAAAKPGKTGSPQGSQAGPPGNVSPTVPVRPGIRLRGPDGPCADFDALTFSPLPG
                     SAAEVRRVQELWGASGDRARGGPVKVLTGREASEANLSRAAVGKKVLHLATHAFFVPS
                     RCEERITAGAVAPKAGVTGGRLQAGTPLRISGLALSGANRRASARSADDDGLLTAEEI
                     SSLDLDGVEWVVLSGCQTGIGLARAGEGILGLRRSFQVAGARTLVLSLWPVSDNDTQS
                     WMAELYRARSRGATTSDAVTAASRRIVEARRKAGVTVHPFFWGAFVAAGDWH"
     gene            complement(6360..6695)
                     /locus_tag="VFD06_04910"
     CDS             complement(6360..6695)
                     /locus_tag="VFD06_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02907.1"
                     /translation="MKTRKALLVALVAVAAIGLAHVVMADPSRTNAEGSVTLAGLPDD
                     SAPACGPAASFETTVDPTLPGSNSSQQPQPMASCQIYASDCVFDGGPCGPQGLCHCQE
                     NPGFWICAR"
     gene            6979..7551
                     /locus_tag="VFD06_04915"
     CDS             6979..7551
                     /locus_tag="VFD06_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN02908.1"
                     /translation="MDNEHAAADIAAFLRGEPEVVAEVRASIRGVVRGFQFRGRDAER
                     DLVQEAVSRVVQNLSGGQFRGESSLRTYAQQLAKYVCLEHIRRQRAEVRLDFESVPSE
                     ARWSEPEETLLREEEHARNMQAFAALPAESRELLRLLFVDGLSYVEAGRRLGLSETAV
                     KSRVHRLRAECRETLARITGAWKRMPGAER"
     gene            7548..>7994
                     /locus_tag="VFD06_04920"
     CDS             7548..>7994
                     /locus_tag="VFD06_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02909.1"
                     /translation="MTAPDDHPDLIAWVSGAMRRVGPGAPATMESEVAAHLRGCEACR
                     TEAEELRSMRRSLRASEESHLAIDTLLEMEAAGIAGFSGKDGAAEKHLSRCGACRADL
                     DALVGAGRARLRMEPERTPAPDRAARRRARLAWAAAAAIAVVGLGVL"
BASE COUNT         1169 a         2799 c         2921 g         1105 t
ORIGIN      
        1 aaggagaaga agatcaccct gcgcggcgcg aaggtggcgg tgcaggggtt cggcaacgcc
       61 ggcagcatcg cggcgcgcct cctgcacgag gacggagcga ccatcatcgc cgtctccgac
      121 agcagcggcg gcatccacag cccgaaggga ctcgacatcg ccgccgtcga ggcgcacaag
      181 gggcgcaccg gcagcctcaa ggggtacgcc gaggcggacg ccatcagcaa cgagcgcctg
      241 ctcgagctgg agtgcgacat cctggtgccg gcggcgctcg aaaaccagat caccacagag
      301 aacgccgcgc gcgtgcgggc gaagatcgtc gccgaggcgg ccaacggccc gaccaccccg
      361 ggggcggacg atatcctctc caggaatggg gtgttcctca tccccgacat cctggcgaac
      421 gcgggcgggg tgacggtctc ctacttcgag tgggtgcaat cgctgcagtc tttcttctgg
      481 gaggagcagc aggtcaacga gcacctcgag aagatcatga cccgctcctt ccacgaggtc
      541 ctggcgctcg cgaagaagca ccaggtgcac atgcgcaccg ccgcctacat cctgggggtc
      601 ggccgcgtcg ccgaggcgac ccgcatccgc ggtatctacc cgtagtgcgc gcgctgctcg
      661 cgaccgccgc ggtggcggcg ctgctcgtgg tggccgcggc gccgccggcg cgcgccgccg
      721 atcctccgat ccgtcccaag gtggtcagcc tgcgcctgaa gatggggctc atggggcaga
      781 tcacgctcta cgccccggcc atgtcgcggc ccgacctcct ccccgtcctc ttcttcgcgg
      841 gggactgggg ctggcggccg ctgcagcagg agtcggcctc ccacctcgcc tccgagggac
      901 gcttcgtcgt cgggatcgac tcgaccgagt acttcaagcg gcgcctggag gcggccgact
      961 gggcggccga tctcaagcag ctgcgcgact ttaccaacga gaaggcggga cgccccgccg
     1021 gcggaccggt cctgctcgtc gggtactgct gggggggaga ggtgatcccc tacatgctca
     1081 accgcggcgg cgcgtcggga ttcgccgggg cggtgctgct cgggcccgac aaggacagcg
     1141 cgttcattta ccgagtgagt ctacagatga cgaacgtccc gtcgccgcca gacgaagcct
     1201 tcaacgtcgc cgaggagctg aagcgtctcc cggcgggctt cccggtcgcg ttcatcgagg
     1261 gggagctgga caccaacagc gcggcgcgca cgctcgaggc gctcgtcccc ggcccgcaca
     1321 agcgcgccgt cgtccccggc ggcgaccgcc agttccgcga ggtccgcaac atctacctcg
     1381 gcctgctgag ccaggcgctg gcgtggatcg aaggccaggg gaagggcgcg ccgccgaccg
     1441 acccgcccgc cgggggccgc tgacgctcga tcagcttctg tcggaagaaa tccgcacttc
     1501 gaatgagtag tagtagggac ccgcttcgcc ccccggcgtc gcgggtttca gcaccacctg
     1561 atacgttccg gacgtcgcga agcgggaggc cggaaccggg atctcgacgc ggcccatcgc
     1621 atcgtgcgga ggcagcgcct ggtcggcgac ggtctcctcg tcgccggggc cgcgcacacc
     1681 gaaccgcagt tccgttcccg gcaccggatg agtgagcgtc aggcgcagga ccgagacgcc
     1741 cggctcgagt tcgacgcgcg gcagtcccgc gccggcgtcg cgcatcggtc cgccgccgag
     1801 gtcgagcatt ttctcgactc cggacggcgc agcgccagca gaatcgacag cggccggggt
     1861 gccgttcccc ggcgggacgg acgcgactcg cgattcggac ggacccaggc ggacgatgat
     1921 gatgccgccc agcaccgccg cggcggtgcc gagtccccac aggaggcgcc cgccccgcca
     1981 ggcggaggcg ggtgcgacgc cgtgcgcctc gagggcctcc gacaggctct gcagctcgcg
     2041 gacgtcgcgc gcgcaggcgg cgcatgcggc gacatgggcg gccaccatcg ccgcctcgtc
     2101 tccttccagg ctcccgttga catggaagtc gagcaactcg ttcgccgggt gtccgcattc
     2161 cggagacgcg tcggtcggcg tcccggtcat cgatctgcga tcctcaggag ccgccgccgc
     2221 agggcgagca ggcggtcgcg gctgcggaag acgcgcgcct tgatcgtccc ttccgcgacc
     2281 cccagcttgc gggcgatctc cgcgtaggag aggtgctcgg cgtgcaccat gtggaggaga
     2341 tgccgctcct ccggggtcga gccgaggagg atcttgcgca gcaggtcgcg cgagatgagg
     2401 cgatccgtcg tctcgtccga ggtgatctcc ctccgccggt cgagggccgg atcggtcgat
     2461 gggtcgcgtc gcgccccggc gcgacgccac gcgtcgatgc aggtgttccg ggcgatccgg
     2521 tgcacgtagg tgcgcagctc cgacgcgccg tggaaccggt ccgagcgcag gttccggaac
     2581 acgcgcatgc ggacttcctg ccggaggtcg tcccattccc tgtgcaggct gtggaactcg
     2641 gtccggagca ccgtctggat ccaggaatcc agcgtggcga tcgactgcac gtgcccgacc
     2701 agcagccggc ggatcagccg gcggtcgtcc tccgaagggg aacggacggg gacagcgacc
     2761 ttctcatcca gcacttcgtc catggcgacg tggggggagc atagggtgaa gtccgcaccc
     2821 gtgcaactgg cttcttgcgc caatccaagg cttttccacc gcgcgacggg ccagggggcc
     2881 ggatccaatc cggacatgga gatcgcagga ttcatgatgt gttagtcgcc gggaccaggc
     2941 cggggttgcc ggatttcacg ggagaagatc ccagcagcgg aggccctccg gacgcggtat
     3001 atagaacgcc gtgagagggc gttcgttccg ggtttcgctc cgcctgctcg cgccggtcct
     3061 cgtctgcctc gttgccggag ggtcgatcgc ctctcctccc ggtacggagg cggagcccgc
     3121 cgacgccgcc gcgctgcgac ccctcatcca tgacgcgcgc tgggcggagg cggagaccgc
     3181 cgcccgcgcc ctggtcgcgc aacgggcggc ggcatacggc gaggactccc ccgagtacgc
     3241 ggccgctctc gagctcctgc tcgaggtgct ggtgcgctcc ggccggtgga gcgaaccgaa
     3301 gaccagggag ctggcggcgc gcgccgtcgc gatcaacgag aagacgctcc cgccggatgc
     3361 gccggcgctg gcgacgagcc tcatccggct gggaggcatc gagcggctgg tcgggccgtt
     3421 cgatcacgcc cgggccatcc tggagcgggc gctcgcgatc cgggaggcgg cgttcggggc
     3481 gaacagcctg ccggtcgccg aggcactcgc ccagctgggc tggtccatga tcgacacccc
     3541 cgatccgcag caggcgatcg cgatgcacga gcgggccctg gcgatccgcg agtcgattct
     3601 cgggaaggac gatctcctgg tcgccgtcag cctggccgga ctgggaggcg tggcgcgcaa
     3661 cgcgggggat ttccagaagg ccgaggcggc gcacgagcgg gccctcgaca tccgccgccg
     3721 tcacctgcgg gccgatcatc cggacatcgc gctgagcatc ctgagcgtcg ccgacgcgaa
     3781 gcaccgcctc ggcgacctcg ggggcgcgct accgctgtac gaggaggccc tgacgctccg
     3841 cgagcacgag cccacgcccg acctgccgcg tctcagcgag gcgcttctca atctgggcga
     3901 cctgctcacc gatctcggca gcctggaccg ggcccggccg atgtacgagc ggggagtccg
     3961 gatccgggac gaaacgatcg gagacggatc cttcatcacg gggatcgggc accagtccta
     4021 cgctctcttc ctggaggcgg ccggggacgc ggccggggcc aggcgcgagt tcgagcgggc
     4081 catcgccatc ttcgatgggg cgacggagcc gggccatccg ctgcgggcgg tcggccggca
     4141 ggacctgggc ctgatgctca agcggcaggg ggatctcgac gcggcgcgcg agatgctgca
     4201 ggcctccctc gacatctggc gcatggcgcc cgcgggcagg ccgggccatc ccttcagcgc
     4261 cgtcccgatg atcgctctcg ggtcgatcct ccgggaccgc ggggacgtgg cggccgctgg
     4321 gccactgctg gaccaggcag tcgcgatcct cgaggcctcg tacgggcccg cgcacccgcg
     4381 ctacgccgaa gcgctcctgg aacggtcacg gcttgccttc cgggagggga acgacgacgc
     4441 ggcgctcgag gacgcgctgc gcgccgagga gtcgcttcgc atcaatcttc gcgccacgac
     4501 gcgcgcgctg tcggagtcgg aggcgttgcg ttatcaggag gtgatgatct ccgggctcga
     4561 cacggccttc tcgatcctgc tcgacgccgg cgacccgccg gatgcgtcca cccatgcgcg
     4621 cacggtcttc gaggcgctcg ggcggtcgcg gacgctggtt ctcgacgaga tcgccgcgcg
     4681 ccatcgcagc atcacccgga gcgacgaccc gaagattcgc agtctcgacg aggcgctcgt
     4741 ggccgccctg cggaattacg cccggctcgt ggtcgagggc ccgagccaac ggtctcccgc
     4801 agggtacgcg ccgcgcctgc agggggcgcg cgaggcgatg gaacgggcgg agcgcggtct
     4861 ggccgccgcg agcagagcgt tcgccgacgc ccgtgagcgg cagaacatga cgtacgctgc
     4921 gatcgcgaag acgcttcccg aggcctcggc cctcgtgtcg ttcgtcgcgt acacgcgatc
     4981 cgatcgcgcg gccggcaagg gaaagccctc gtacctcgcc tgcgtcgtcg cgccgggagc
     5041 ggccgccccc gcggtggttc ctctcggcgc ggcggctggg atcgacgctg ccatcgatcg
     5101 ctggaggaag gagtcgtcga cgccgcccgc cggtgccgcc gcggaggagt cgtaccgcct
     5161 cgcggcggcg gcggtccggg aggcgctctg ggatccgctg gccggacggc tgggcagcgc
     5221 gcgccgcgtg ttcatcgtgc cggacgccgc catccacgca ctgtcgttcg cgaccctcgt
     5281 cgatcgcggc gggaagtacc tcatggagac cgggccggag ttccattacg tgtcctccga
     5341 aagggacctg ctcgaaggcg ccggcgggcc ggtgcgcggc agcggattgc tggcgctcgg
     5401 cggccccgat ttcgacgccg cggcgaagcc cgggaagacc ggttccccgc agggaagcca
     5461 ggcgggaccc ccgggcaacg tgagcccgac ggtgccggtc cgcccgggca tccggctgcg
     5521 gggccccgac gggccgtgcg cggacttcga cgcgctcacg ttcagcccgc ttcccggatc
     5581 ggccgctgag gtgcggcggg tgcaggagct ctggggtgcc tcaggggatc gcgcccgcgg
     5641 gggaccggtg aaggtgctca ccgggcgcga ggcgagcgag gcgaatctca gccgcgccgc
     5701 cgtcgggaag aaggtcctgc acctcgcgac gcacgccttt ttcgttccga gccggtgcga
     5761 ggaaaggatc accgccgggg cggtggcccc gaaggccggt gtcacaggag ggcgcctgca
     5821 ggccgggacc ccgctgcgca tctcggggct ggcgctgtca ggggcgaacc ggcgcgccag
     5881 cgccaggtcg gccgacgacg acggcctcct cacggccgag gagatctcgt ccctcgatct
     5941 cgacggcgtc gagtgggtcg tcctgtccgg atgccagacc gggatcggcc tggcgcgcgc
     6001 cggcgaaggg atcctcgggc tgcgccgctc cttccaggtg gcgggcgcgc gcacgctcgt
     6061 cctcagcctg tggccggtga gcgacaacga cacgcagtcc tggatggcgg agctctaccg
     6121 cgcgcgttcc cgaggcgcca ccacctccga cgccgtgacc gcggccagcc gccgcatcgt
     6181 cgaggcgcgc cgcaaggcgg gggtcacggt tcaccccttc ttctggggag ccttcgtcgc
     6241 cgccggcgac tggcactgac acgccggcga ggccgggttc gggcagaacg gccaagccgg
     6301 aaaacacgaa ggggccccga cctccggggc cccttctcga ttcgcaggcc tggttcgaac
     6361 tagcgggcgc agatccagaa gccgggattc tcctgacagt ggcagagacc ctgcggaccg
     6421 cagggaccgc cgtcaaaaac gcaatcactc gcgtagatct ggcaggaagc catcggctgc
     6481 ggctgctggc tcgagttgga accggggagg gtcggatcga cggtcgtctc gaaagaggcc
     6541 gccggtccgc aggccggcgc ggagtcgtcg ggaagcccgg ccagcgtgac cgatccctcg
     6601 gcgttcgtgc gggacggatc cgccatcacc acgtgagcga gaccgatcgc cgcgacggcg
     6661 accagcgcga ccaggagagc cttgcgcgtc ttcatcgtgc tcctcccttc gggttgagaa
     6721 gacctgctcg tgcacttgga aaccggcagc gactccggcc gcgcttctag tgtcgcgagc
     6781 cggtggaagg tcgcagcgtt gccgcgggcg gaacggcagg gcggcgctcc accctccacg
     6841 cccgcggacc gatccgcgaa gacacaaccc ccaacgtgaa gaacccttgt agtgtcacga
     6901 acaccgcaaa ggtcgctaac tttccgtcat ccaggccgac tcatacagta gatttcggga
     6961 cggatacgcc gcgacatcat ggacaacgag cacgccgccg ccgacatcgc cgcgttcctg
     7021 cgcggcgagc cggaggtcgt cgcggaggtg cgcgcgtcga tccgcggcgt ggtgcgcggc
     7081 ttccagttcc gcggccggga cgccgagcgg gatctcgtcc aggaggcggt gagccgcgtc
     7141 gtccagaacc tcagcggcgg ccagttccgc ggcgagtcgt cgctccgcac gtacgcgcag
     7201 cagttggcca agtacgtctg tctcgagcac atccggcgcc agcgcgcgga ggtgcggctg
     7261 gacttcgagt cggtcccctc ggaggcgcgc tggtccgagc cggaggagac gctcctccgc
     7321 gaggaggagc acgcgcgcaa catgcaggcg ttcgcggcgc tgccggccga gtcgcgcgag
     7381 ctgctgcggc ttctgttcgt cgacggcctg tcctacgtcg aggcggggcg gcggctgggt
     7441 ctcagcgaaa ccgcggtcaa gtcgcgcgtc caccggctgc gcgccgagtg ccgcgagacg
     7501 ctggcgcgga tcacgggcgc gtggaagagg atgcccggag cggagcgatg acggcgcccg
     7561 atgatcatcc cgacctgatc gcctgggtgt ccggcgccat gcgccgggtc ggaccaggcg
     7621 ctcccgcgac gatggagagc gaggtcgccg cgcacctccg cggctgcgag gcgtgccgca
     7681 ccgaggcgga ggagctgcgc tcgatgcggc ggagcctgcg cgcctccgag gaatcgcacc
     7741 tcgcgatcga cacgctcctc gagatggaag cggccgggat cgcaggattc tcggggaagg
     7801 acggtgccgc cgagaagcat ctgtcgcggt gcggcgcgtg ccgcgccgat ctcgatgcgc
     7861 tcgtgggcgc cggccgcgcg cggctccgga tggagccgga gcgaacgccg gcgccggatc
     7921 gggccgcccg ccgccgcgcg cggctcgcct gggcggcggc cgcggcgatc gccgtcgtcg
     7981 gcctcggcgt gctg
//
LOCUS       DASRIQ010000199         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3202515, whole genome shotgun sequence.
ACCESSION   DASRIQ010000199 DASRIQ010000000
VERSION     DASRIQ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3202515"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="VFD06_04925"
     CDS             <1..1442
                     /locus_tag="VFD06_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZN02910.1"
                     /translation="EDPLLVVNANLLMGVDLRQMIRAHRESKASVTVGVVPLHGDARP
                     RPQRERLHLAEDGGLRGLEVDYERVPQEDSYRMAGIYLFDKASLRQIPAGVYFDLKEQ
                     FLPALLASGRPVHTHPLSGYLRELNSAEDYLLAHFDLCNGRTGSSRIGEELADGMWVQ
                     GVVDLPPDVIVIGPVVLGPGVVVGSGARLVGPLVIGRGSEIGEGAHLRETVIGEEVSV
                     GPRARVERSILADGLRVGEGAVLKDSLATSQPLSVGDLNLVERDLRIGVASLPFERYL
                     GVGARRRGYSAIKRAFDFVFASLAFVVALPILIAVALATAWDSGFPILFRQRRCGRDG
                     RPFTMVKFRSMRPDAEQLRQGLRERNEMDGPVFKISNDPRFTRVGRFLRRYSLDELPQ
                     LWNVLRGDMSIVGPRPLQDAEMRVCPSWREARLRVKPGLTGLWQVSSREKGSFQDWIQ
                     HDLRYVRTQSFFLDLKIVVRTILALAKGL"
     gene            1482..1772
                     /locus_tag="VFD06_04930"
     CDS             1482..1772
                     /locus_tag="VFD06_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN02911.1"
                     /translation="MTVRSGARTGQNGTNGDKAVSGDRGRNRASPGPDAAEQILTSRE
                     ARAFLRIGRTKLHELTRRQIIPAYRIGEGRTSSLRYLRSDLLGWLVSQRVSR"
     gene            1784..2953
                     /locus_tag="VFD06_04935"
     CDS             1784..2953
                     /locus_tag="VFD06_04935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN02912.1"
                     /translation="MPRVLLVVNSLGYGGTERMVERLVTALEGRGDVLFTIVTLEGKG
                     PIGARLQKDGRDVRPLALTGGAARQILGGRRALRRLLRSERFDLIHSFLYRSHSACRL
                     ARIGARPAPPLLSSERCLGDNRGFGIRVVNRLTGAWSDRVLAVSRAVAETVVRRDRVP
                     EEKVAVVPNGIEEAEADPRRRSRLRRALGVRDGDVLLLFLGRLHAEKGPDLMLDALER
                     LRRIAPHGWRAALVGDGPLRDALARRIAGSGLADRVVQPGARRFVGPWLDAADLLVLP
                     SREEGMPVAALEAMMRGRPVVGTAVGGTPEVVDHGRTGLLVPAGDGEALARALADLIG
                     DAARRRAMGAEAAAEARRRFTLERMADATLAEYRALLRAAEATLAKSAPAPARID"
     gene            2953..>3645
                     /locus_tag="VFD06_04940"
     CDS             2953..>3645
                     /locus_tag="VFD06_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HZN02913.1"
                     /translation="MCGIAGYWNHKSGRPADRGVVAAMTRALRHRGPDDEGFFLDGPL
                     ALGMRRLSIIDIAGGHQPIGNEDGRVQLVFNGEIYNHRALRRDLEAAGHRFRTHADTE
                     VVVHGYEIHGERVLDRLNGMFGLALWDARARRLLLARDRLGVKPLYWTRIPEGVAFAS
                     ELKALLLHPAVERRFDPESLRQYLAWEYIPAPRTPFTGIHKLPPAALLAIDASGTRQE
                     TYWRLRPAPEITD"
BASE COUNT          473 a         1281 c         1343 g          548 t
ORIGIN      
        1 tcgaggatcc gctcctcgtg gtgaacgcga atctcctcat gggggtcgat ctgcgccaga
       61 tgatccgcgc ccaccgcgag tcgaaggcct ccgtcacggt cggcgtggtg ccgctgcacg
      121 gcgatgcgcg cccgcggcca cagcgcgaac gtctgcatct cgccgaggac ggcggactgc
      181 gcgggctcga ggtcgactac gagcgcgtgc cccaggaaga ctcctaccgc atggcgggga
      241 tctacctctt cgacaaggcc agcctgcggc agattccggc cggcgtctat ttcgatctca
      301 aggagcagtt cctgccggcg ctgctcgcga gcggccgccc ggtgcatacg cacccgctgt
      361 cgggctacct gcgcgagctg aacagcgccg aggactacct tctggcgcac ttcgatctct
      421 gcaacggccg caccggttcg tcgcgcatcg gcgaggagct ggcggacgga atgtgggtgc
      481 agggggtcgt cgatctgccg ccggacgtca tcgtgatcgg cccggtcgtg ctcggtccgg
      541 gcgtcgtcgt ggggtcgggg gcgcgactcg tcggcccgct ggtcatcggc cgcgggtcgg
      601 agatcggcga gggggcgcac ctgcgcgaaa cggtgatcgg cgaagaggtg agcgtcgggc
      661 cgcgggcgcg tgtcgagcgt tcgatcctcg ccgacggcct gcgggtgggc gagggggcgg
      721 tcctgaagga cagcctggcg acgtcgcagc cgctgtcggt cggcgatctc aacctggtgg
      781 agcgggacct gcgcatcggc gtcgcctcgc tgccgttcga gcgctatctc ggggtggggg
      841 cccggcgccg aggctactcc gcgatcaagc gcgccttcga cttcgtcttc gcgtcgctgg
      901 cgttcgtcgt cgcgctgccg atcctgatcg ccgtcgcgct cgccaccgcc tgggactccg
      961 gcttcccgat cctgttccgt cagcggcgct gcggccgcga cggacgtccg ttcaccatgg
     1021 tgaagttccg ctcgatgcgc ccggatgccg agcagctccg ccagggcctg cgcgagcgca
     1081 acgagatgga cggaccggtg ttcaagatct ccaacgatcc gcgcttcacg cgggtcggcc
     1141 gcttcctccg gcgctacagc ctcgacgagc tgccgcagct ctggaacgtg ctgcgcggcg
     1201 acatgagcat cgtcgggccg cgcccgctgc aggacgcgga gatgcgtgtc tgcccttcgt
     1261 ggcgcgaggc gcggctgcgc gtgaagccgg ggctgaccgg gctctggcag gtctcgtcgc
     1321 gcgagaaggg gagtttccag gactggatcc agcacgacct gcgctacgtg aggacccagt
     1381 cgttcttcct cgatctgaaa atcgtcgtgc gcaccatcct ggcgctggcc aagggtctct
     1441 agaccgcttc ctttcagagc atgatcagga caaaacggac catgacggtg cgatccggag
     1501 cgcgaacggg ccaaaacggt acgaacgggg acaaggcggt ctccggcgac cggggccgga
     1561 accgcgcgtc cccgggaccg gacgccgcgg agcagatcct cacgagccgc gaggcgcgcg
     1621 ccttcctccg catcgggcgg acgaagctcc atgagctgac gcgccggcag atcatcccgg
     1681 cctaccgcat cggcgaggga cggacctcct cgctgcgcta cctgcgcagc gacctgctcg
     1741 gatggctggt gagccagagg gtgtcgcggt gaggcgcgcc ggcgtgcccc gcgtgctcct
     1801 ggtggtgaac agcctcggct acggcggaac cgagcggatg gtggagcggc tggtgaccgc
     1861 cctcgaaggt cgcggggacg tcctcttcac gatcgtcacg ctggagggga aaggtccgat
     1921 cggcgcgcgc cttcagaagg acgggcgcga tgtccgcccg ctcgccctga cgggcggggc
     1981 ggcgcggcag attctcggcg ggcgccgcgc gctgcggcgg ctcctgcgct cggagcggtt
     2041 cgacctgatc cacagcttct tgtatcgttc gcattccgcc tgccggctgg cgcgcatcgg
     2101 cgcgcggccg gcgcccccgc ttctctcgtc ggagcgctgc ctgggggaca accgcggctt
     2161 cgggatccgc gtcgtcaacc gcctcaccgg cgcgtggagc gatcgggtcc tggccgtgtc
     2221 gcgcgccgtg gcggagaccg tggtgcgccg cgatcgggtg ccggaggaga aggtggcggt
     2281 ggtcccgaac gggatcgagg aggcggaggc ggacccccgg cggcgctccc ggctgcggcg
     2341 cgcgctcggc gttcgggatg gcgacgtgct cctcctgttc ctcgggcggc tccacgccga
     2401 gaaggggcca gatctcatgc tcgacgcgct cgaacgtctg cgccggatcg ccccccatgg
     2461 ctggcgggcc gcgctggtgg gggacggccc gctccgcgac gccctcgcgc ggcggatcgc
     2521 cgggtccggc ctggccgatc gcgtcgtcca gccgggggcg cggcgtttcg tcggcccctg
     2581 gctcgatgcc gctgacctcc tggtcctgcc gtcacgcgag gaggggatgc cggtggccgc
     2641 gctcgaggcg atgatgcgcg gacgcccggt ggtcggcacc gcggtcggtg gcactcccga
     2701 ggtggtggac cacggccgga ccggcctcct ggttccggcc ggggacggcg aggcgctcgc
     2761 ccgggcgctc gccgatctga tcggggacgc ggcgcgccgg cgcgcgatgg gagcggaggc
     2821 ggcggccgag gcgcgacggc gcttcacgct cgagcgcatg gccgacgcga cgctcgccga
     2881 ataccgggcc ctgctccgcg ccgccgaggc cacgctcgcg aaaagcgctc cggcccccgc
     2941 gaggatcgac tgatgtgcgg catcgcgggt tactggaacc acaagagcgg ccgtccggcc
     3001 gatcgcggtg tcgtcgcggc gatgacccgg gcgctccggc accgcggccc cgatgacgag
     3061 gggttcttcc tcgacggtcc gctggcgctc gggatgcgcc gcctgagcat catcgacatc
     3121 gccggcggac accagccgat cggcaacgag gacggccggg tccagctggt cttcaacggc
     3181 gagatctaca accatcgcgc cctgcgccgc gatctggagg ccgccgggca ccgtttccgc
     3241 acccacgccg acaccgaggt cgtcgtgcac ggctacgaga tccacgggga gcgcgtcctc
     3301 gaccgcctga acggcatgtt cggcctggcg ctctgggacg cccgcgcccg gcgccttctc
     3361 ctggcgcggg atcgcctggg cgtgaagccg ctctactgga cccgcatccc ggagggtgtc
     3421 gccttcgcct cggagctcaa ggcgctgctg ctccacccgg cggtggagcg ccgcttcgat
     3481 cccgagtcgc tccgccagta tctcgcctgg gagtacatcc ccgcgccgcg cacccccttc
     3541 accggcattc acaagctgcc gccggcggcg ctcctggcga tcgacgcctc ggggacgcgg
     3601 caggagacct actggcgact gcggccagcg ccggagatca ccgac
//
LOCUS       DASRIQ010000200         8453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2705585, whole genome shotgun sequence.
ACCESSION   DASRIQ010000200 DASRIQ010000000
VERSION     DASRIQ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2705585"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VFD06_04945"
     CDS             complement(<1..1346)
                     /locus_tag="VFD06_04945"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HZN02914.1"
                     /translation="MNFSGTKPRLLLVLLAQRADSASVWGALERIPQPLLGRMESIVV
                     MAGEDLAPVLEEQSRARDKALAERIVVERDPRRYGYGGRRKAALEYARGRAVDVVAVA
                     DASVDLPLERLAELFGAALEPEAPIVIASRSRPGRPAGAGFAHNFLLGFRLHDYASGF
                     RLLPRVWLQRLPFELNADDRRFDTEILIQARAAGARIVEVPVPPFGDGLPAEDRFSIA
                     RSIGAAVDYRLHQLHVTRRGQYLVEAAAPYLFKRSPHGSHMQVLDAIAAHTRVLDLGC
                     SQGLLAGPLRARDVRVVGVDAHDPAPVSPDLEAYYQRDLEEPLVLPEGRSFDYVVVSD
                     VIEHIRNRAQLLRSVRRNLKPDGRLLISTPNVAIWFYRLSLLAGRFEYGPRGVLDETH
                     VHLFTRATFRREIERAGFRVLRERVTALPFEVVFESTGQSRLLRLASAASHLLARL"
     gene            1482..2003
                     /locus_tag="VFD06_04950"
     CDS             1482..2003
                     /locus_tag="VFD06_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZN02915.1"
                     /translation="MGSKFHATRLSIDPTAWVAPGAALVGEVTIAARASVWFQATVRG
                     DLAPIEIGEESNVQDGCVLHVERDGPVRLGRRVTLGHGAIVHGAVVEDEVLVAMKAVV
                     LSGCHIGRHSIVGAGAVVPEGTRVPEGSLVLGVPGKVVRPLRPEEIERIVRNAASYVA
                     LAAAYRGGEVLVP"
     gene            2000..3031
                     /locus_tag="VFD06_04955"
     CDS             2000..3031
                     /locus_tag="VFD06_04955"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZN02916.1"
                     /translation="MSGAAKGEGRDDEFSASAAERLKPLLATAKTAVLATHVTPDGDG
                     IGAEICLQAYLADRGIEARIFNTEATPLRYRFLDERNAVEVYDEAAHGEFLRSADLLF
                     MLDNSAPSRFGPLEKAARASRAVTVCIDHHNITDPFWKVNLIDSSAAATGEMVYRIVE
                     ALGGRPDFTAAQAAYVSLVTDTGSFRFGKTAPRSHRLAADLLEAGVSPPRVHEEVYER
                     SSAALVRLAGVALAGLSLGEGNRLGWITLTLQQVRDSGGMNEDTSDVVNELLTIDGVR
                     LAVLLKELEGGKLKLSFRSKGTLDVNRIAQSFGGGGHTNASGAVIAGTIAERLEEILQ
                     PCRALLQSG"
     gene            3004..3468
                     /locus_tag="VFD06_04960"
     CDS             3004..3468
                     /locus_tag="VFD06_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02917.1"
                     /translation="MPGAAPERVGGSGPPESIPPLVLRCRTPYILIVGGAAAALLALA
                     TGCLAAGGPEGPVYAAVLFAGGAITTHFFLRYCLAALELSDRGFRLRGPLHAGVEVGW
                     RDVRAWRRRGAPLGPGFLRIEPHERPRITIPLLYEDAHLLELGLTQERFPVW"
     gene            complement(3392..4357)
                     /locus_tag="VFD06_04965"
     CDS             complement(3392..4357)
                     /locus_tag="VFD06_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZN02918.1"
                     /translation="MSLRRLLARPGTVMLDGATGTELQRRGIDTRLPLWSARALTSDE
                     GRRVLAGIHREYTEAGAEILTTNTFRTTPRALERAGLLQHWTMLNRRAVEAARAGAGK
                     DGLVAGSLAPLEDCYEPDRVPLQETCLREHLRQADLLARLGVDLFLVETMNCRREARA
                     AILAARSTGLHLLLALCPGAPDRLLSGEPLEDALPRLLDDGGGRILGVLLNCAPPEVL
                     ERAAPLLDRVLGTLPWGLSAHLGHADPEDGWRLPEEHAPDAYADWAAARVREGARLAS
                     GCCGTTPAHIARLTARLGDAPTRPENAPGSGPARAGARPRTAAES"
     gene            complement(4354..6225)
                     /locus_tag="VFD06_04970"
     CDS             complement(4354..6225)
                     /locus_tag="VFD06_04970"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZN02919.1"
                     /translation="MDAAALKSDKIRVLFQPRDATTRVPPGTTLFHAAAWIGQPVEST
                     CGGRGTCGKCKVRILSGPAPVSTADQHHLGSEEIASGWRLSCQAEVHAETVVEVPRLL
                     GTPKTAMFGVGRQVLLDPNVHKVHLKLVPPELHDQRSDLQRLKEALEAEGYPLVADLQ
                     ALRILPKALRDADFDITAVLCGADLVAIEPGDTTQASYGAAFDLGTTTIVGTLLDLNT
                     GEARAVASSLNAQAVHGGDVLSRISYTMGKREGLGELQRMAVLTFNTLLEDLGRQTGV
                     PADRIYEVAIAGNATMQHLLLGLDPEPISVTPFAPVVTRGVEVRADLLGLAIHPNGRA
                     YLFPHLGAYVGGDIVAGLLATAVPRGDRLRLFVDVGTNGEIALGWEKRTISTAAPAGP
                     AFEGAEISCGMRATRGAIEGVRIGEDAVTLQTIGDAAPVGLCGSGLIDAVAQLYRRGL
                     LDATGRLRSAEEVRAKVPESLASRLITLDGVRAFVLATPDETGGTAVLLSQKDIRQLQ
                     FAKGSIATGIQVLMNEMGVAAAELQEVLLAGAFGSYIHPDSARTIGLVPPVPLERIRA
                     VGNAAGEGAKIALLSYREREAAEGMPLRVEYLELSGREDFNDLFMKALGFPPLASMP"
     gene            complement(6239..7114)
                     /locus_tag="VFD06_04975"
     CDS             complement(6239..7114)
                     /locus_tag="VFD06_04975"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZN02920.1"
                     /translation="MKTVVSSATRTVVIGPDEPFAIIGERINPTNRKKLAEELRRLDM
                     GRVRQDAKAQVEAGAHLLDVNAGIPGADEPGMLTAAVRAIMETVDVPICIDSSTPEAL
                     EAALPAYQGKALVNSVTGEDEVLDRLLPLIAKHKAAVIGIANDDTGISNDPRERLAIA
                     RKIVERAGDHGIPPEDVVIDPLCMPIGANHTFGRITLETMRLIRDELGVNMSIGAGNV
                     GFALPDRPPLTAAFLVLAMAHGLTAAITNPIEAEIRKAILAADLMLGHDPMGRRWVAH
                     YRKAAAKPAATGPAA"
     gene            complement(7114..8094)
                     /locus_tag="VFD06_04980"
     CDS             complement(7114..8094)
                     /locus_tag="VFD06_04980"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZN02921.1"
                     /translation="MTASAAATRRLEEAFASDRPVLTCEIAAGDSPDPSGLLKRAALV
                     RDHVDAVNVPDNTAGIAHMSGLAAAAILASAGVEPILHVTCRDLNRIGLQSLLVGAAA
                     LKVRNLLCLTGDHMAHGDQPQAKPVFDLDSLQLLRLAGTVRSGRYLSGRPIVPAPDLF
                     PGTTENPFAPPYEFRPLRLRKKIEAGARFVQTQIVYNVPRFREFMTRVVDLGIDRQAP
                     ILAGVSPLRSARGARHMRDHIPGMDVPAALVRRMEGAGDDRARAEEEGLAICLEILEE
                     LRTIPGLRGFHLMPIHWDEAVPEIVSRARLRGPEAVPALPAATRLQEARA"
     gene            complement(8091..8402)
                     /locus_tag="VFD06_04985"
     CDS             complement(8091..8402)
                     /locus_tag="VFD06_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZN02922.1"
                     /translation="MLIKKPIFGCRMCGQCTLHETGLTCPMGCPKTLRNGPCGGVRLD
                     GRCEVIPEMACIWVKAERRARFLPWRGAILRIQPALDWTQRGHSSWVNALKGTAREAR
                     P"
BASE COUNT         1200 a         2909 c         3164 g         1180 t
ORIGIN      
        1 cacagccgcg ccaggaggtg agacgcggcc gaggccaggc gcagcaggcg gctctggccg
       61 gtcgactcga agaccacctc gaacgggagg gcggtcacgc gctcgcgcag gacgcggaag
      121 ccggccctct cgatctcccg ccggaaggtg gcgcgcgtga acaggtgcac gtgggtctcg
      181 tcgaggacgc cgcgcggccc gtactcgaag cggcccgcga ggagcgacag acggtagaac
      241 cagatcgcga cattgggagt cgagatgagg agccggccgt ccggcttgag attgcggcgg
      301 acgctgcgca ggagctgggc gcggttgcgg atgtgctcga tcacgtccga gaccacgacg
      361 tagtcgaagg agcgtccttc gggcaggacg aggggctctt cgaggtcgcg ctggtagtag
      421 gcctcgagat ccggcgagac cggcgccggg tcgtgggcgt ccacccccac cacgcgcacg
      481 tccctggcgc gcagcggccc cgcgaggagc ccctgcgagc agccgaggtc caggacgcgc
      541 gtgtgcgctg cgatggcgtc gagcacctgc atgtgcgagc cgtgcgggct gcgcttgaag
      601 aggtacggag ccgcggcctc gacgaggtac tgtccgcggc gcgtcacgtg gagctggtgc
      661 aggcgatagt cgacggcggc gccgatgctc cgcgcgatcg agaagcgatc ctccgccggc
      721 agcccgtcgc cgaacggggg gaccggcacc tcgacgatgc gcgcgccggc cgcgcgcgcc
      781 tggatcagga tctccgtgtc gaagcgccga tcgtcggcgt tcagctcgaa gggcagcctc
      841 tggagccaga cgcgcggcag gaggcggaag ccgctggcgt agtcgtgaag cctgaagccg
      901 agcagaaaat tgtgcgcgaa cccggcgccg gccgggcgac ccgggcgcga ccgggaggcg
      961 atgacgatcg gggcctcggg ttcgagcgcc gcgccgaaca gctccgccag gcgctcgagc
     1021 ggcaggtcca ccgacgcgtc ggcgaccgcg acgacgtcga cggcgcgccc gcgggcgtat
     1081 tcgagcgctg ccttgcgccg gccgccgtag ccgtaacggc gcggatcgcg ctcgacgacg
     1141 atccgctccg cgagggcttt gtcccgggcg cgggactgct cctccaggac gggggccaga
     1201 tcctctcccg ccatcacgac gatgctctcc atgcggccga ggagaggctg cgggattcgc
     1261 tccagggcgc cccagacgga ggcgctgtcc gcccgctgcg ccaggagcac gagcaggagc
     1321 cggggcttcg tgccggagaa attcaagccg agagtctatg tgcggtcagg gctggtccgt
     1381 caacctcgac tctgcggccg agtgtcgtcg aagcgttccg ttttgttgac acggggggaa
     1441 gcgtggccta tattcgccgg cttcgcggag aagacggcgg gatgggttcg aagttccacg
     1501 cgacgagact gagcatcgat ccgacggcgt gggtggcgcc gggggcggcg ctcgtcggcg
     1561 aggtgacgat cgcggcgcgc gcctcagtct ggttccaggc gacggtgcgg ggcgatctgg
     1621 cgccgatcga aatcggagag gagagcaacg tgcaggacgg gtgcgtgctg cacgtcgagc
     1681 gggacggacc ggtgcggctg gggcggcgcg tcaccctggg ccatggagcc atcgtgcacg
     1741 gcgcggtcgt ggaggatgag gtgctcgtgg cgatgaaggc ggtggtcctg agcggttgtc
     1801 acatcgggcg gcattcgatc gtcggcgccg gggcggtcgt gccggaaggg acacgcgtcc
     1861 cggaggggtc gctcgtgctc ggcgttcccg ggaaggtcgt gcgcccgctc cgccctgagg
     1921 agatcgagcg gatcgtgcgc aacgccgcct cctatgttgc gctggccgcc gcctatcgcg
     1981 gcggcgaggt cctggtccca tgagcggcgc cgcaaagggc gaggggcgcg acgacgagtt
     2041 ctccgcgagc gcggcggaaa ggctcaagcc gctcctcgcc accgccaaga cggcggtcct
     2101 ggcgacgcac gtgaccccgg acggcgacgg catcggggcc gagatctgcc tgcaggcata
     2161 cctcgcggac cgcggtatcg aggcgcgcat cttcaacacg gaggcgacgc ctctgcgcta
     2221 ccgcttcctc gacgagcgga acgcggtcga ggtgtatgac gaggcggcgc acggggagtt
     2281 cctgcgctcc gccgacctgc tcttcatgct cgacaattcc gcgccgtcgc gcttcggacc
     2341 gctggaaaag gctgcacggg cctcgcgcgc cgtcaccgtg tgcatcgatc accacaacat
     2401 caccgacccg ttctggaagg tgaacctgat cgattcgtcc gcggccgcca ccggcgagat
     2461 ggtgtaccgg atcgtcgagg cgctcggagg tcgcccggac ttcaccgcgg cgcaggcggc
     2521 ctacgtgtcg ctcgtgaccg acaccgggtc gttccgcttc ggcaagaccg cgccgcgctc
     2581 gcaccggctg gcggcggacc tcctcgaagc gggcgtcagc ccgccgcgcg ttcacgagga
     2641 ggtctacgag aggagcagcg ccgcgctggt gcgcctcgcc ggcgtggcgc tcgccgggct
     2701 cagcctcggg gaagggaacc ggctcggctg gatcaccctg acgctccagc aggtgaggga
     2761 cagcgggggg atgaacgagg acacgtcgga cgtcgtcaac gagctgctca ccatcgacgg
     2821 cgtccggctg gcggtcctgc tcaaggagct cgaaggcggg aagctgaagc tgtccttccg
     2881 cagcaagggg acgctcgacg tcaatcgcat cgcgcagtcg ttcggcggcg gcggccacac
     2941 caatgcttcg ggggcggtga tcgccgggac gatcgcggag cgcctcgagg agatcctgca
     3001 gccgtgccgg gcgctgctcc agagcgggta ggcgggagcg gcccccccga gagcatcccg
     3061 cccctggtcc tgcgctgccg gaccccgtac atcctgatcg tcggcggtgc cgcggccgcg
     3121 ttgctcgcgc tcgccaccgg ctgcctcgcc gccggcggac ccgaaggccc ggtctacgcc
     3181 gccgtcctgt tcgcgggagg cgcgatcacg acgcacttct tcctgcgcta ctgcctcgcg
     3241 gcgctcgagc tgtcggatcg cggcttccgg ctccgcgggc cgctccacgc cggggtcgag
     3301 gtcggatggc gtgatgtgcg cgcctggcgt cgtcgcggcg cgccgctcgg cccgggcttc
     3361 ctccggatcg agccgcacga aaggccgagg atcacgattc cgctgctgta cgaggacgcg
     3421 cacctgctcg agctgggcct gacccaggag cgttttccgg tctggtaggc gcatccccga
     3481 ggcgggcggt gaggcgcgcg atgtgcgccg gcgtcgtgcc gcagcaaccg gaggcgagac
     3541 gggccccctc gcgcacccgc gccgccgccc aatcggcgta ggcatccggc gcgtgctcct
     3601 ccgggaggcg ccatccgtcc tccggatcgg cgtggccgag gtgcgccgac agtccccagg
     3661 ggagggtgcc gagcacgcgg tcgagcagcg gcgccgcacg ctccagcact tcgggcggcg
     3721 cgcagttgag cagcacgccc aggatccggc cgccgccgtc gtcgaggagc cgcggcagcg
     3781 cgtcctcgag cggctcaccg gacagaagtc gatcgggcgc tccggggcag agggcgagaa
     3841 ggaggtggag cccggtgctg cgcgcggcga ggatcgccgc ccgcgcctcg cggcggcagt
     3901 tcatggtctc gacgaggaac aggtcgacgc cgaggcgcgc cagaagatcg gcctgacgca
     3961 gatgctcccg caggcaggtc tcctgaaggg gaacacggtc cggttcgtag cagtcctcga
     4021 gcggcgccag cgaaccggcg accagcccgt ccttcccggc gccggcgcgc gccgcctcga
     4081 cggcgcggcg gttgagcatc gtccagtgct ggaggagccc ggcccgctcg agcgcccgag
     4141 gcgtcgtccg gaaggtgttg gtggtgagga tctccgcgcc cgcctcggtg tactcccggt
     4201 ggatgcccgc gaggacgcgg cgtccttcgt cgctcgtcag ggcgcgcgcc gaccagaggg
     4261 ggagccgcgt gtcgatcccg cggcgctgca gctcggtgcc ggtggcgccg tcgagcatca
     4321 ccgtccccgg gcgggccagg agccggcgca ggctcacggc atcgacgcga gcggcgggaa
     4381 ccccagggcc ttcatgaaca ggtcgttgaa gtcctcgcgc cccgacagct cgaggtactc
     4441 gacccgcaag ggcatccctt ccgccgcctc gcgctcgcgg taggagagga gggcgatctt
     4501 cgccccttcg cccgcggcgt tgccgacggc gcggatccgc tcgagcggca ccggcgggac
     4561 gaggccgatg gtgcgggcgg aatcggggtg gatgtaggag ccgaacgccc cggccagcag
     4621 cacttcctga agctcggccg cggcgactcc catctcgttc atcagcacct ggatgccggt
     4681 ggcgatcgat cccttggcga actgcagctg gcggatgtcc ttctggctca ggaggacggc
     4741 ggtgccgccg gtctcgtccg gggtcgcgag gacgaaggcc cgcaccccat ccagggtgat
     4801 cagccgcgat gcgagggact cggggacctt ggcgcggacc tcctcggccg agcgcagccg
     4861 accggtggcg tcgagaagcc cgcgacgata cagctgcgcc accgcgtcga tgagccccga
     4921 tccgcacagc cccacggggg ccgcgtcgcc gatggtctgg agggtcacgg cgtcctcgcc
     4981 gatgcgcacc ccttcgatgg ctccgcgggt ggcccgcatc ccacaagaga tctccgcccc
     5041 ctcgaaggcc ggcccggcgg gggccgccgt cgagatcgtc cgcttctccc aaccgagggc
     5101 gatctcgccg ttggtgccga cgtcgacgaa cagccgcagg cgatcgccgc gcgggaccgc
     5161 ggtcgcgagc agcccggcga cgatgtcgcc gccgacgtag gcgccgaggt gcgggaacag
     5221 gtaagcgcgg ccgttcgggt ggatcgccag gccgaggaga tcggcgcgca cctcgacgcc
     5281 gcgcgtcacc accggcgcga acggggtcac cgagatcggc tccgggtcga gcccgagcag
     5341 gaggtgctgc atcgtggcgt tgccggcgat cgccacttcg tagatccggt cggccgggac
     5401 tcccgtctgc cggccgagat cctcgagcag cgtgttgaag gtgaggaccg ccatgcgttg
     5461 cagctccccc aggccttccc gtttccccat cgtgtaggag atccggctca gcacgtcgcc
     5521 gccgtgcacc gcctgggcgt tgagcgagga ggcgacggcg cgcgcctcgc cggtgttgag
     5581 gtcgaggagc gtcccgacga tggtcgtggt cccgagatcg aacgccgcgc cgtaggaggc
     5641 ctgcgtcgtg tcccccggct cgatcgccac gaggtcggcg ccgcacagga ccgcggtgat
     5701 gtcgaaatcc gcgtcccgca gcgccttcgg gaggatgcgg agagcctgca ggtcggcgac
     5761 cagcgggtac ccctccgcct cgagcgcctc cttcagccgc tgcagatcgg agcgctgatc
     5821 gtgcagctcg ggtgggacga gcttcaggtg caccttgtgg acgttgggat cgagcagcac
     5881 ctgccggccg acgccgaaca tcgcggtctt cggggtcccg aggagccgcg gaacttcgac
     5941 gacggtctcg gcgtgcacct ccgcctggca cgacaggcgc cagccggagg cgatctcctc
     6001 ggagcccaga tggtgctggt cggcggtcga gaccggggcc ggccccgaga ggatccgcac
     6061 cttgcacttg ccgcaggtgc cgcgcccgcc gcacgtggac tcgaccggct ggccaatcca
     6121 ggccgcggcg tgaaagaggg tggtgccggg tgggacgcgg gtcgtcgcgt cgcgcggctg
     6181 gaacagcacg cggatcttgt ccgatttcag cgctgcggcg tccatgggtc cctccgcctc
     6241 aggcggcggg ccccgtcgcg gcgggcttcg ccgccgcctt gcggtagtgc gccacccagc
     6301 gccggcccat cgggtcgtgg cccagcatga gatccgcggc gaggatggcc ttgcggatct
     6361 cggcctcgat gggattggtg atggcggccg tcaggccgtg cgccatcgcc aggacgagga
     6421 aggcggcggt cagcggcggc cgatccggaa gcgcgaagcc gacgttgccg gcgccgatcg
     6481 acatgttgac accgagctcg tcgcggatga gccgcatcgt ctcgagagtg atgcgcccaa
     6541 aggtgtggtt ggcgccgatc ggcatgcaca gcgggtcgat gacgacgtcc tccggcggga
     6601 tgccgtgatc gcccgcccgc tcgacgatct tgcgcgcgat cgccaggcgc tcgcgggggt
     6661 cgttgctgat gccggtgtcg tcgttggcga tgccgatgac cgccgccttg tgcttggcga
     6721 tgagcggcag caggcgatcg agcacctcgt cctcgccggt gaccgagttg accagcgcct
     6781 tcccctggta cgcggggagc gccgcctcca gcgcttcggg ggtggaggaa tcgatgcaga
     6841 tcggcacgtc gacggtttcc atgatcgccc gcaccgccgc ggtgagcatc cccggctcgt
     6901 ccgcccccgg gatgccggcg ttgacgtcga gcaggtgcgc ccccgcctcg acctgtgctt
     6961 tggcgtcctg ccgcacccgt cccatgtcga gacgccgcag ctcctcggcg agcttcttgc
     7021 ggttggtcgg attgatccgc tcgccgatga tcgcgaacgg ctcgtccggg ccgatgacga
     7081 cggtgcgcgt ggcggaggag acgacggtct tcatcaggcg cgtgcctcct ggagccgggt
     7141 cgcggccggg agcgcaggga cggcctccgg cccccgcaga cgggcgcgcg acacgatctc
     7201 cggcaccgcc tcgtcccagt gaatcggcat gagatggaac cccctgagcc cggggattgt
     7261 acgcaattcc tccaggatct cgagacagat cgccagcccc tcttcctcgg cccgggcgcg
     7321 atcgtcgccg gctccttcca tgcggcggac gagcgccgcc ggcacgtcca tcccggggat
     7381 gtgatcgcgc atgtggcgcg cgccgcgcgc cgagcgcagc ggggagacgc cggcgaggat
     7441 cggcgcctgc cggtcgatcc cgaggtcgac gacccgcgtc atgaactcgc ggaagcgggg
     7501 gacgttgtac acgatctgcg tctggacgaa gcgcgccccc gcctcgatct tcttgcgcag
     7561 ccgcaggggg cggaactcgt agggaggcgc gaagggattc tcggtcgtcc ccgggaagag
     7621 atcgggtgcc gggacaatcg gccgccccga gaggtagcgc cccgagcgca ccgtgcccgc
     7681 gaggcgcagc agctgcagcg agtcgaggtc gaacaccggc ttggcctgcg gctggtcgcc
     7741 gtgcgccatg tgatcgccgg tgagacagag gagattgcgc accttgagcg ccgccgcccc
     7801 cacgagcagg ctctgcaggc cgatgcggtt caggtcgcgg caggtgacgt gcaggatcgg
     7861 ctcgacgccg gccgacgcga ggatcgccgc cgccgccagc cccgacatgt gggcgatccc
     7921 ggcggtgttg tccgggacgt tcaccgcgtc gacgtgatcg cgcaccagcg ccgcccgctt
     7981 caggagaccc gaaggatcgg ggctgtcccc ggcggcgatc tcgcacgtca ggaccggccg
     8041 gtccgaggcg aacgcctcct ccaggcgccg cgtggccgcg gcggaggcgg tcacggacgc
     8101 gcctcgcgcg cggttccctt gagggcgttg acccaggagg agtggccgcg ctgggtccag
     8161 tcgagcgccg gctggatgcg caggatggcg ccgcgccagg gaaggaagcg cgcgcgccgc
     8221 tccgccttca cccagatgca ggccatctcc gggatcacct cgcaccggcc gtccagccgc
     8281 acgccgccgc acggtccgtt gcgcagcgtc ttcgggcatc ccatcgggca ggtgagcccg
     8341 gtctcgtgca gggtgcactg cccgcacatg cggcagccga agatcggctt cttgatcagg
     8401 atctcgagag gccgggcggc gcgatcgagg aaccgcgacc acgccgggtg ccg
//
LOCUS       DASRIQ010000201         3882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1896691, whole genome shotgun sequence.
ACCESSION   DASRIQ010000201 DASRIQ010000000
VERSION     DASRIQ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896691"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VFD06_04990"
     CDS             <1..1443
                     /locus_tag="VFD06_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HZN02923.1"
                     /translation="VYLGIHVISRKVIFVDIALAQIAALGGTVAFLLGYDPRSEGAYW
                     CSLAFAVLAAGVFALTRTRTERVPQEAVIGLTYATASAAAILLADISPHGAEHLHDLL
                     AGSIVWVTPAQIVKTAVLYALLGLFHFAFRKRFLQISLHPEAAYAAGVRVRLWDFLFY
                     LSFGVVITSSVQIAGVLLVFCYLVAPSIFAVMFYENLRPRLVTGWIMGTLVSAIGLAF
                     SYDRPSGPTIMVVFAVALLTGALVRAVLRGARPAVGLAATAVVLATLTGAAASLQASR
                     LASAAPEGAAAAAEAAAARAGHAPGEAAAGDAHEQGEGIEGLRRALRDAHAEVRSRAV
                     AGLARTGDPRVVHDLLDALGDQDAGVREAAAAALGQMGSRSAVTALRQHLDHRDEDPW
                     VRLRMAESLGSLGEPAALPVLVELASGGEGKVLRLEALQILSRLTNLGDSALDDPDDE
                     AAQTRLAKARAFLKASGGRPRFDASTRTFR"
     gene            complement(1495..1938)
                     /locus_tag="VFD06_04995"
     CDS             complement(1495..1938)
                     /locus_tag="VFD06_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02924.1"
                     /translation="MLEALARALQRPADRVTVLDEAEGERLAARFEERWRQAGASDDP
                     ERALSTSTPDRLVVESLFGWLAARLPQGRELIFLLRIDGRTLPARATAQEALVAAPEL
                     VREIGDAVHLMFPGGEFGLALEMVSDAVPGGDGRIYKVFVWGAPR"
     gene            complement(2147..2887)
                     /locus_tag="VFD06_05000"
     CDS             complement(2147..2887)
                     /locus_tag="VFD06_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN02925.1"
                     /translation="MQSPVVLVTHSRKLYRRMRSAAPPGALLRVGCLFPAGPGEAAAT
                     VMVDPRAVDSTCLRTLSGWRRDRASHHVVYLAFHGSPDRLITLPRVHPGPYLDVDRAI
                     EHLRRLQIGPAPAPAWDDWAQILEAAGGNERARVFLSLALRASGHNAVVDAAAALHVG
                     PRQLTRLCRRWFGHPPAAIIRQARVVRAIPDLLVSGLSLTQIARRHGFSSRQAMTRLF
                     EISSGHPPRAFRRPPDEEVRGADAGAAC"
     gene            3187..3636
                     /locus_tag="VFD06_05005"
                     /pseudo
     CDS             3187..3636
                     /locus_tag="VFD06_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
BASE COUNT          520 a         1432 c         1419 g          511 t
ORIGIN      
        1 gtctatctcg ggatccacgt catcagccgc aaggtcatct tcgtggacat cgcgctggcg
       61 cagatcgccg cgctcggggg aacggtggcg ttcctcctcg ggtacgaccc gcgatcggag
      121 ggggcctact ggtgctcgct ggcgttcgcg gtgctggcgg ccggggtgtt cgcgctgacc
      181 cgcacgcgca ccgagcgcgt tccccaggaa gccgtgatcg gcctgaccta cgccaccgcc
      241 tccgcggcgg cgatcctgct cgccgacatc tccccgcacg gggcggagca cctgcacgac
      301 ctgctcgccg gcagcatcgt ctgggtgacc ccggcgcaga tcgtcaagac ggcggtgctg
      361 tacgctctgc tcggcctctt ccacttcgcc ttccggaagc gcttcctgca gatctcgctg
      421 cacccggagg cggcgtacgc cgcaggggtg cgggtgcgcc tgtgggactt cctcttctac
      481 ctctccttcg gcgtcgtcat cacctcgtcg gtgcagattg ccggcgtgct cctggtcttc
      541 tgctacctcg tggccccgtc aatcttcgcg gtgatgttct acgagaacct ccgcccgcgc
      601 ctcgtcaccg ggtggatcat ggggaccctc gtcagcgcca tcggtctcgc cttctcctac
      661 gaccggccgt cgggtccgac catcatggtg gtgttcgccg tggcgctcct gaccggagcg
      721 ctcgtccgcg cggtgctccg gggcgcgcgc ccggcggtgg ggctcgccgc aacggccgtc
      781 gtcctggcga cgctgacggg cgccgcggcg tccctgcagg cgagccgcct ggcgagcgcc
      841 gcgccggagg gggctgccgc cgcggccgag gcggcggccg cccgcgccgg acacgcgccg
      901 ggggaggcgg cggccggaga cgcgcacgag cagggggagg ggatcgaggg gctgcgccgc
      961 gccctgcgcg acgcccatgc cgaggtgcgg tcgcgggcgg tggccggcct ggcgcgcacc
     1021 ggggacccgc gcgtggtgca cgacctgctc gacgcgctcg gggaccagga cgccggagtg
     1081 cgggaggcgg cggccgccgc gctgggtcag atgggaagcc gctcggcggt cacggccctc
     1141 cgacagcacc tcgatcaccg cgacgaggat ccctgggtgc gactgcgcat ggcggaaagc
     1201 ctcggatcgc tcggcgagcc ggcggcgctg ccggtgctcg tcgagctcgc ctcgggaggg
     1261 gaagggaagg tcctgcgcct cgaggccctt cagatcctgt cgcggctcac gaacctcggg
     1321 gacagcgccc tcgacgatcc ggacgacgag gcggcgcaga cgcgtctggc gaaggcgcgc
     1381 gctttcctca aggccagcgg cggccggccg cgcttcgacg cctccacccg cacgttccgc
     1441 tgagcccggc gtcacggacg gacaccggcg cggatcgcgc cgggcggcca gcagctagcg
     1501 cggggcgccc cagacgaaga ccttgtagat ccgcccgtcg cccccgggaa cggcgtcgga
     1561 gaccatctcg agcgccagcc cgaactcgcc gccggggaac atcaggtgca ccgcgtcgcc
     1621 gatctcgcgc acgagctcgg gggcggcgac aagcgcctcc tgcgccgtgg cgcgtgccgg
     1681 cagggtccgg ccgtcgatgc gcagcaggaa gatcagctcg cgcccctgcg gcaggcgggc
     1741 cgccagccat ccgaagagac tctcgacgac caggcggtcc ggcgtcgagg tggacagggc
     1801 gcgctcggga tcgtcgctgg cgcccgcctg tcgccagcgt tcctcgaagc gggcggcgag
     1861 ccgttcccct tccgcttcgt cgagaacggt cacgcgatcg gccggccgct gcagggcccg
     1921 cgccagcgcc tcgagcatcc ccggagggcg tccgcgctcc ccgcgcctgg gggacgcgcg
     1981 tttcgagcgg gacccctccg aatcccctcg cgccagtgtc atgtcccggg accgtaacaa
     2041 aggacgcgcg cgggcggaag ggtccgtttt cggacctggg ggatcgttcc cggacatacc
     2101 gccggggcgt ccgccgggaa acgaccggac agatgccggc cgcctctcag caggccgccc
     2161 cggcgtccgc gccacgcacc tcctcgtcgg gggggcggcg gaaagcgcgt ggcggatgtc
     2221 cggacgagat ttcgaagagc cgcgtcatcg cctgccggct gctgaacccg tggcgccgcg
     2281 cgatctgcgt gaggctgagc cccgagacca gcagatcggg gatcgcccgc accacgcgcg
     2341 cctgccggat gatcgccgcc ggcggatgcc cgaaccatcg ccggcacaac cgggtcagct
     2401 ggcgcggacc gacgtgcagc gccgccgccg cgtcgacgac cgcgttgtgt cccgacgcgc
     2461 gcagcgcgag actcaggaag acgcgggcgc gttcgttgcc gcccgccgcc tcgaggatct
     2521 gcgcccagtc gtcccaggcc ggggccggcg ccggcccgat ctggagacgc ctgagatgtt
     2581 cgatcgcccg atcgacatcg agatacgggc ccggatggac ccgcggcagc gtgatgaggc
     2641 gatcggggct gccgtggaac gcgaggtaga ccacgtggtg cgacgcgcgg tcgcgccgcc
     2701 agccggagag cgtccgcagg caggtcgaat cgacggcgcg cgggtccacc atgacggtcg
     2761 ccgccgcctc cccgggcccg gccgggaaga gacagccgac gcgcaggagc gcaccgggag
     2821 gcgcggcgga gcgcatccgc cggtacagct tgcgcgagtg ggtgacgagc acgaccgggc
     2881 tctgcatggg tccctccgcg ccgaccagtc ttgcggcgcg gatgtccaag agcagcgttc
     2941 gtgccagccg gccccgacct ccggctcggc cgcgaaacca cgtgcgatca acgacttgac
     3001 cttttccacc tggagtctca cgatgccgcc gcgccctgcg tgaccgtctc acgagactct
     3061 cacgagactc gagcgggccg cgcgaggcag gggccgcgcg ggacgccttg agcggcggcg
     3121 cggggcgata ggagcctgtc caggtgcgat acgatgcgcg gcgcacgagt tcccggagga
     3181 tccgcgatgc ctcccttcac cctcaagatc aacggcgcct cccgccgcgt cgacgtcccg
     3241 gccgacatgc cgctcctgtg ggtgctccgc gacacgctcg gcctcaccgg caccaagttc
     3301 ggctgcggcg agtccctgtg cggcgcctgc acggtccacc tcgacggcct gccggtgcgc
     3361 tcctgcgtca ctccggtgtc cgaggcggcg caggcgcagg cgatcaccac catcgaaggg
     3421 ctctcgccgg atcgcagcca cccggtgcag aaggcctgga tcgaggagga cgtcccgcag
     3481 tgcggctact gccagcccgg gatgatcatg tcggcggcgg ccctcgtccg cgagaacacc
     3541 tcccccaccg acgccgacat cgacgaggcg ctcggcgggc acatctgccg ctgcggcacc
     3601 taccagcgca tccgcgccgc cgtgaagcgg gcggcgcgcg ccgccgccgg tccgcagggg
     3661 ccgaagggag ggcgtcgccg ttttgaggcc gccccagggg ctcccggacc aaagccggga
     3721 acagggaaac ctccgggcgg cacgacacct ggaggaggct cgtcatgagc cgcctcgtga
     3781 acatcccgcg gcgcgagttc ctgcgcatcg tgccggcggc gggcgccggg ctcgtcatcg
     3841 ggttccggat cggaggcgac gcacgggcgg accagccggc cg
//
LOCUS       DASRIQ010000202         4984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_80804, whole genome shotgun sequence.
ACCESSION   DASRIQ010000202 DASRIQ010000000
VERSION     DASRIQ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80804"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..448
                     /locus_tag="VFD06_05010"
     CDS             173..448
                     /locus_tag="VFD06_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02926.1"
                     /translation="MKAKNAMGFAVAAAVVGMLATGYQFTADEKKDEGSVKCAGINSC
                     KGHGACAAADGSHACAGKNECKGKGWVKVKSDKECTDKGGTVIKDAK"
     gene            512..1387
                     /locus_tag="VFD06_05015"
     CDS             512..1387
                     /locus_tag="VFD06_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HZN02927.1"
                     /translation="MRPASLGHGVGLRREHFERVLSAPTRIDWFEVISENFMADGGRP
                     VDVLDQVRARYPIVLHGVSLSIGSTDPLNKPYLDRLAALARRTEPAWVSDHLCWAGVG
                     GHYAHDLLPLPYTEEALRHVVGRVDQVQEHLGRPIALENVSSYVTFRQSSMPEWEFLA
                     EIARRSGCGILLDVNNIYVSSRNHGFDPRAYVEGIPKDCVWQFHLAGHADKGKYLLDT
                     HDHPVPDPVWELYALAVERFGAVSALVEWDDRLPDFERLEQESLRARQIEAGILHPAT
                     AGASHPKAAAGEAAP"
     gene            1384..2379
                     /locus_tag="VFD06_05020"
     CDS             1384..2379
                     /locus_tag="VFD06_05020"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HZN02928.1"
                     /translation="MSGPSTLSRTESLFWDLITAPEGVRPGLDALVRDGRAAQGALEE
                     MIAGDDRLPAAERLDIYANMYFFRLLDCLREDYPRLATAVGEDRFHNLCTDYLLTHPS
                     GHPSLRYLGRRLPEFLAGHVVGRESGCLVDLARLEWTRADLFDAADARALTREDLARL
                     SQEAAGDLPLRPVPAFRLLRLDHDAPRLWRELRDREAAGPETPGEGGAATGTETAEAT
                     WGAGAANAARLGEETDGARCASGHVHDDRPAPPLPRQRRRKTAVRVWRREFTVYHRAV
                     EDEEAALLELLTEGTTLGQVAQRLSAGRSQAKATEALGRVLQAWIDAGLLAVEPR"
     gene            2547..4667
                     /locus_tag="VFD06_05025"
     CDS             2547..4667
                     /locus_tag="VFD06_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN02929.1"
                     /translation="MNSAKPPAARVQEVTDTVHGVRIADPYRWLEDGSSNEVRAWVDG
                     QNAWAEALLGARPGRAGVRERLGRLLAIGTIGTPDVRGDLYFFTRREGTQNQPILYVR
                     QGLKGQDRVLVDPNPLSPDGTATIDWWFPSRDGKLLAYGISTAGDEKSTLRVRDTATG
                     RDLPDVIPHTRYCSLGWVPDGSGFYYTRYPTPGSVPAGQENYNRRVFHHRLGDDPAND
                     PLVFGADRKPEEIIEIDLSADGRWLTAVIYDGWARTDLFVSDRKAQSPTFTAVAQGLD
                     AIFTGGVVHGILYVRTNFEAPRYRLLAIDPSKPGRDGWKTLIPQGAPVLDDARVVGGT
                     IVARLMQQASSRVVLYDRDGRRLTEIPLPALGTVDSISGEPDGKEVFFGFSSYTVPPR
                     VERIDLATKTASTWQKVTADVDLSRLEVKQVFYPSKDGTKVSMFVVGRKGLALDGSNP
                     TLLYGYGGFNVSQTPGFSRSLVLWLERGGVYAEANLRGGGEYGEDWHRAGMLDRKQNV
                     FDDYIAAAEWLIAKKYTRSDRLAIYGGSNGGLLVGAALTQRPDLFKAVVCAVPLLDML
                     RYQNFQIARLWVPEYGSAEDAAQFKYLYAYSPYHHVKKGTAYPATLLTTADSDSRVDP
                     MHARKMAALLQASTSSDRPILLRTETRAGHGAGKPLSKQIDEAADVYGFLIWQLGLEM
                     KRDLAPEPYPESGSNATKAGGDRS"
     gene            4664..>4984
                     /locus_tag="VFD06_05030"
     CDS             4664..>4984
                     /locus_tag="VFD06_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014875141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN02930.1"
                     /translation="MSVSVTAGKPHVAHTVCPLDCPDRCSLDVTVDAGRVTAIDGSRV
                     NPVTDGFICGKVRHFTKRLYGPDRLLHPMRRTGQKGSGTFEAISWEEAIATIAARLAQ
                     ARNES"
BASE COUNT          779 a         1758 c         1705 g          742 t
ORIGIN      
        1 cccctttgca aggggggacg gcataccccc cgggaatccc cgacagagga cagggagggg
       61 atttgcctcc ccgaggggtg atgtgtaata tccggcgttc acggaatagc ctgtagtccc
      121 tgtctcaact gcggttgcag tcaaccagct gtctcgaaga ggaggaaggg caatgaaagc
      181 aaagaatgcg atgggcttcg cggtcgctgc ggctgtggtc ggcatgctgg ctaccggcta
      241 ccagttcacg gcggacgaga agaaggacga ggggagcgtc aagtgcgccg gcatcaactc
      301 ctgcaaggga cacggtgcct gcgccgccgc cgacggcagc catgcctgcg ccgggaagaa
      361 cgagtgcaag ggcaagggct gggtcaaggt caagtccgac aaggagtgca cggacaaggg
      421 cggcacggtc atcaaggacg cgaagtaagc gacatcacgc cgcggggacc ggctccctcc
      481 ccgggatccg gttcccgcgg cttttcattt catgcgcccc gcctccctcg gccatggcgt
      541 agggctgcgc cgtgagcact tcgagcgtgt cctctccgct cccacccgca tcgattggtt
      601 cgaggtcatc tccgagaact tcatggccga cggcgggcgg ccggtggacg ttctcgatca
      661 ggtgcgcgcg cgctatccaa tcgtcctgca cggcgtatcg ctgtcgatcg gctccaccga
      721 tcccctgaac aagccctatc tcgaccgcct ggccgcgctg gcccggcgca ccgagccggc
      781 ctgggtctcc gatcacctct gctgggcagg ggtcggaggc cattacgccc atgacctgct
      841 gccgcttcct tatacggagg aggcgctgcg tcacgtggtc ggccgggtcg atcaggtgca
      901 ggagcacctg ggccgcccga tcgccctgga gaacgtctcc tcgtacgtga ccttccgcca
      961 gtcgagcatg cccgagtggg agttcctggc ggagatcgcc cgccgttcgg gctgcggcat
     1021 cctgctcgac gtcaacaaca tctacgtctc gtcgcgcaac cacggcttcg acccgcgtgc
     1081 ctacgtcgag gggatcccga aggactgcgt ctggcagttc cacctcgccg gccacgccga
     1141 caagggcaag tacctgctcg acacccacga ccacccggtg ccggatcccg tctgggagct
     1201 ctacgccctg gcggtcgagc ggttcggcgc ggtcagcgcc ctcgtcgagt gggacgatcg
     1261 gctccccgac ttcgagcggc tcgaacagga gtcgctccgc gcgaggcaga tcgaggctgg
     1321 catccttcat ccggccactg ccggcgcttc gcatccgaaa gcggccgccg gggaggccgc
     1381 cccatgagcg gcccctccac gctgagccgg accgaatccc tcttctggga cctcatcacg
     1441 gcgccggaag gagtgcgccc gggtctcgac gccctggtgc gggacggccg cgcggcccag
     1501 ggcgccctcg aggagatgat tgcgggcgac gatcgtctgc cggcggcgga gcggctcgac
     1561 atctacgcca acatgtactt cttccgcctc ctcgactgtc tgagggagga ctacccgcgt
     1621 ctcgcgaccg ccgtcgggga ggaccgcttc cacaatctct gcaccgatta cctcctgacg
     1681 cacccgtccg ggcacccgtc actgcgctat ctcgggcggc gcctgccgga gttcctcgcc
     1741 gggcacgtgg tcgggcgcga gtcgggatgc ctcgtcgatc tcgcccgcct ggaatggacc
     1801 cgggccgacc tgttcgatgc cgccgacgcg cgggcgctca cccgggagga tctcgcccgc
     1861 ctttcccagg aggcggcggg ggatctgccg ctccggccgg tgccggcgtt ccgtctgctg
     1921 cggctcgatc acgatgctcc ccggctgtgg cgcgagctgc gcgaccgcga ggccgccggc
     1981 ccggaaacac cgggcgaagg gggagcggcc acgggcacgg agacggccga ggcgacctgg
     2041 ggagccgggg ccgccaacgc cgcgcggctc ggtgaagaga ccgacggggc gcgctgcgcc
     2101 tcgggccacg tgcacgatga tcgcccggct ccgccgcttc cgaggcagcg ccgaagaaag
     2161 accgccgtcc gggtctggcg gcgcgagttc accgtctatc accgcgccgt cgaggacgag
     2221 gaagcggccc tgctcgagct cctgacggag gggacgaccc tcggccaggt ggcgcagcgg
     2281 ctgagcgcag ggcgctccca ggcgaaagcc accgaagcgc tgggtcgggt tcttcaagcc
     2341 tggatcgacg ccggcctgct ggctgtcgag ccccgctgac cggggtgccc gcggcgtccg
     2401 ccacttccta ttcctgggat agaatcaccg ctcctgaccc ggagcgtggc gtgtccgttc
     2461 ggccccgcac ccatcgaccg ttttccgcgc gtgccgcggc atgcgccgcg ctcgcgtgcg
     2521 tcggggccgc ggagatcctc ctcgccatga acagcgccaa gccgccggcc gcccgcgtcc
     2581 aggaagtgac cgacaccgtc cacggcgtgc ggatcgccga tccgtaccgc tggctcgagg
     2641 atgggtcctc gaacgaagtg cgggcctggg tcgacgggca gaacgcctgg gccgaagccc
     2701 tcctcggagc gcgcccgggg cgggcgggag tgcgggagcg gctcggccgg ctcctggcca
     2761 tcggcacgat cggcaccccg gacgtgcgcg gcgacctgta cttcttcacc aggcgggagg
     2821 ggacgcagaa ccagccgatc ctctacgtcc gtcaggggct gaagggacag gaccgggtgc
     2881 tcgtcgatcc gaaccctctg agtccggatg ggaccgccac catcgactgg tggtttccgt
     2941 cgcgcgacgg aaagctcctg gcgtacggca tctcgaccgc gggcgacgag aagagcacgc
     3001 tgcgggtgcg cgacacggcg accggccgcg atctccccga cgtcatcccg cacacgcgct
     3061 actgctctct ggggtgggtc cccgacggca gcggcttcta ctacacccgt tacccgacgc
     3121 ccggctcggt gccggccggc caggagaact acaaccggcg tgtcttccac catcggctcg
     3181 gcgacgatcc ggcgaacgac ccgctcgtgt tcggcgccga tcgcaagccg gaagagatca
     3241 tcgagatcga tctctccgcc gacggccgct ggctgaccgc ggtcatctac gacggctggg
     3301 cccggaccga cctcttcgtc agtgacagaa aggcgcaaag ccccacgttc accgccgtgg
     3361 cacaagggct cgacgccatc ttcaccggcg gcgtcgtgca cggcatcctg tacgtccgca
     3421 ccaacttcga ggcgccgcgc taccgcctgc tggcgatcga tccgtcgaag ccggggcggg
     3481 acggctggaa gaccctcatc ccgcaggggg cgccggtcct cgacgatgcc cgcgtcgtcg
     3541 ggggaacgat cgtcgcccgg ctgatgcagc aggcgtcgtc gcgcgtcgtg ctgtacgacc
     3601 gggacggccg gcgcctgacg gagattccgc tcccggcgct cggcacggtg gactcgattt
     3661 cgggcgagcc ggacggcaag gaggtcttct tcggtttctc ctcctacacc gtaccgccgc
     3721 gcgtcgagcg gatcgacctg gcgacgaaga ccgcctccac ctggcagaag gtgaccgccg
     3781 atgtcgatct ctcccgcctc gaggtgaagc aggtcttcta cccttcgaag gacggtacga
     3841 aggtctcgat gttcgtcgtg gggcgcaagg ggctggcgct cgacggctcg aacccgacgc
     3901 tcctctacgg ctacggcggc ttcaacgtca gccagacccc cggcttcagc cgctccctgg
     3961 tgctgtggct cgagcggggc ggggtctacg ccgaggcgaa cctgcgtggc ggcggtgagt
     4021 acggcgagga ctggcaccgc gccggcatgc tggatcggaa gcagaacgtc ttcgatgact
     4081 acatcgccgc cgccgagtgg ctgatcgcca agaagtacac gcggtccgat cgcctggcga
     4141 tctacggcgg gagcaacggc gggctcctcg ttggggcggc gctgacgcag cgccccgatc
     4201 tcttcaaggc ggtggtgtgc gccgtgccgc tcctcgacat gctgcgctac cagaacttcc
     4261 agatcgcccg gctgtgggtg cccgagtacg gctccgcgga ggacgcggcg cagttcaagt
     4321 acctctacgc ctactccccc tatcaccacg tgaagaaagg gacggcgtac ccggcgacgc
     4381 tcctgacgac cgccgacagc gacagccgcg tggacccgat gcacgcgcgc aagatggcgg
     4441 cgctgctgca ggcgtccacg tcctccgacc ggccgatcct cctgcgcacg gagacgcgcg
     4501 ccggccacgg cgccggcaag cctctgtcga agcagatcga cgaggcggcc gacgtgtacg
     4561 gcttcctgat ctggcagctc gggctcgaga tgaagcggga tctcgccccc gagccatacc
     4621 cggagagcgg cagcaacgcc acgaaggccg gaggagatcg ctcatgagcg tctcggtgac
     4681 cgccgggaag ccgcacgtcg cccacaccgt ctgcccgctc gactgccccg atcgctgcag
     4741 cctcgacgtc accgtggacg cggggcgcgt cacggccatc gacggcagtc gcgtcaaccc
     4801 ggtcaccgac gggttcatct gcggcaaggt gcgccacttc acgaagcgcc tgtacggccc
     4861 tgaccggctg ctccacccga tgcgccgcac cgggcagaag gggagcggca ccttcgaggc
     4921 gatctcctgg gaggaggcga tcgcgacgat cgccgcgcgg ctcgcgcagg cgcggaacga
     4981 gagc
//
LOCUS       DASRIQ010000203         4285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_341981, whole genome shotgun sequence.
ACCESSION   DASRIQ010000203 DASRIQ010000000
VERSION     DASRIQ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341981"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VFD06_05035"
     CDS             complement(<1..522)
                     /locus_tag="VFD06_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HZN02931.1"
                     /translation="MSWDGEEVLREHDAETGAWFTIAIHSTRLGPAVGGTRMKPYPSP
                     EEAEADARRLSAAMTLKMAASGMPWGGGKGVIAAPPGLADEARCGLLRRYGRLVGSLG
                     GRYRIGPDVGTSAEDMDIVAETASPYVFGCTEARGGSGPSGPATALGVHAAIRIACEE
                     AFGSASLAGRHVLV"
     gene            complement(562..3186)
                     /locus_tag="VFD06_05040"
     CDS             complement(562..3186)
                     /locus_tag="VFD06_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN02932.1"
                     /translation="MPLPPGTTLGPYTIVGPLGAGGMGEVYRARDARLHRDVAVKVLP
                     ASFAADGERLRRFEQEARATGQLNHPNIVAVYDIGTHGGTPYVVEELLEGDTLRARLA
                     GGPLPARKAIDSARQIAIGLAAAHQKGIVHRDLKPENLFVTNDGRVKILDFGLAKLTR
                     PEAGAVAPEALTSAPTAAGTDAGVILGTVGYMSPEQVRGLPADHRSDIFSFGCILYEM
                     LSGRRAFSASSSVETMNAILKEDPPDLGRSTSDLPPGLERIVLHCLEKSPDERFQSAR
                     DLGFQLEALSAISAGSIAGAIAGAGKSTGTAVGGARSGLLGARVLLWPAIAAAVLLAA
                     AGYWAGLSSRPSETEASYTQMTFRPGMITSARYAPDGETVVYSAAWDGKPTAIFTTRA
                     GSPESRAVDLPPADVLSISRSGELAILLDSHFTIGWMRQGTLARASLAGGAPREVMQD
                     VQDADWAPDGESLAVVRTVGGRYHLEYPTGKVLYETDTWVTGPRFSPDGKLIAFVDHP
                     SMGDDRGRIGVVDLAGQVRFLTDAFSSARGVAWTPNGRAVWFSAGRTGNVRAVYEVDL
                     QGRQRMVDGAPTGMTLGDVSASGRILIVRDSARRGIAGTSPGDPVERDLSWLDWSRPA
                     ALSRDGRQVLFEEQGQGGGPGYTVYLRPTDGGPAIRLGRGSALDLSPDGRWAMTTTIG
                     DESLLSFLPTGPGEPRTLRVPGFQVINAQFHPDGRRILLLATESGHPPRFYLLDPDAS
                     GPPRPVTPEGVGIYCAPSSDGRSVAAALAGGAAQLWPIDGGEPVPLSGSQPGDLPTQW
                     SEDGKTVYVVQKQEKAARIDALDVASGRRTPIRTLVPADRAGLIAVDFVQISTNTGAY
                     VYSYRRILSALYQVDGLR"
     gene            complement(3283..3714)
                     /locus_tag="VFD06_05045"
     CDS             complement(3283..3714)
                     /locus_tag="VFD06_05045"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN02933.1"
                     /translation="MNTDKVVLQAEERFFAALSEGNRQALGEIVADDCILIDVLTGSE
                     VPRLDFVDLVGSRRLVFESIVRMPARVRLYGGTAIVTGQTRMTGRFDAQTFQVRSRYT
                     HVYVQHRGGFRLVNAQGTPVPAAAAIVTGAVRDPRLREAGW"
     gene            4006..>4285
                     /locus_tag="VFD06_05050"
     CDS             4006..>4285
                     /locus_tag="VFD06_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN02934.1"
                     /translation="MTKPFDVSDDEARLQALRRTSLLDSPPEEAFDRLTRTATTVLRV
                     PMAIVSLVDRDRLFFKSQSGLAEPLASLRQGPIQHSFCQYAVGTREPLV"
BASE COUNT          630 a         1470 c         1564 g          621 t
ORIGIN      
        1 cacgagcacg tggcgaccgg cgagcgaggc cgagccgaag gcctcctcgc aggcgatgcg
       61 gatcgccgcg tggacgccga gcgccgtcgc ggggccagac ggtccagagc caccgcgcgc
      121 ctcggtgcaa ccgaagacat acggggacgc ggtttcggcg acgatgtcca tgtcctccgc
      181 cgaggtgccg acgtcggggc cgatgcggta gcggccgccg agcgagccga cgagccggcc
      241 gtaacgcctc agcaggccgc agcgcgcttc atccgcgagc ccgggcggcg ccgcgatgac
      301 ccctttccct cctccccacg gcatgcctga agccgccatc ttgagcgtca tcgcggccga
      361 caggcggcgg gcgtcggcct ccgcctcctc gggggaggga tacggcttca tgcgcgtacc
      421 gccgacggcg gggccgagcc gcgtcgaatg gatggcgatc gtgaaccacg cgccggtctc
      481 agcgtcgtgt tcgcgcagca cctcttcgcc gtcccagctc atccgcccat tctaacgggg
      541 gcgggcaaag ggtcgttccc gctagcgcag cccgtccacc tggtacagcg ccgacaggat
      601 gcgccggtag gaatagacgt aggcacccgt gttggtggag atctggacga agtccaccgc
      661 gatcaggccg gcgcggtcgg cggggaccag ggtgcgaata ggggtccggc gcccgctcgc
      721 gacgtccagg gcatcgatgc gggcggcctt ctcttgcttc tgaacgacgt acaccgtttt
      781 cccgtcctcg ctccactggg tcggcaggtc gcccggctgc gacccggaga gcggaacggg
      841 ctcgccgcca tcgatcggcc agagttgcgc cgcgccaccg gccaatgcgg cggcgacgct
      901 gcggccgtcg gacgagggtg cacagtagat gccgaccccc tcgggggtga ccggccgcgg
      961 cggcccggat gcgtcgggat ccagcagata gaagcgcggc gggtggccgc tctcggtcgc
     1021 gagcaggagg atccgccggc cgtcgggatg gaactgtgcg ttgatgacct ggaagccggg
     1081 aacgcgcagc gtgcgcggct cgccggggcc ggtcggcagg aagctcagga ggctctcgtc
     1141 gccgatcgtc gtggtcatgg cccagcgtcc atccgggctg aggtcgagcg ccgaaccgcg
     1201 cccgaggcgg atcgccggcc cgccgtcggt cgggcgcaga tacacggtgt agccggggcc
     1261 gcccccctgt ccctgctcct cgaacaggac ctgacgtccg tcgcgcgaca gggctgccgg
     1321 gcgcgaccag tcgagccacg acaggtcacg ctcgaccgga tcgcccgggg acgtgccggc
     1381 gatgccgcgt cgcgcgctgt cgcggacgat gaggatccgg ccggacgcgg agacgtcccc
     1441 gagcgtcatt cccgtgggcg ccccgtccac catgcgctgc cggccctgga ggtcgacctc
     1501 gtacacggca cgcacattgc cagtgcgccc ggcgctgaac cagacggccc ggccgttcgg
     1561 cgtccaggcc actccgcgcg cggacgagaa cgcgtcggtc aggaaacgca cttgacccgc
     1621 cagatccacg acgccgatcc gcccgcggtc gtcgcccatc gacgggtgat cgacgaaggc
     1681 gatgagcttt ccgtcagggg agaagcgcgg ccccgtcacc cacgtgtcgg tttcgtagag
     1741 gaccttgccg gtcgggtatt cgaggtggta gcgcccgccg acggtgcgca cgaccgcgag
     1801 gctctctccg tccggtgccc agtcggcgtc ctggacgtcc tgcatcacct cgcgcggcgc
     1861 cccgccggcg agggaggcgc gcgccagcgt cccctggcgc atccagccga tcgtgaaatg
     1921 cgagtcgagc agaatcgcca gctctcccga tcgggagatc gagaggacgt cggcgggcgg
     1981 caggtccacc gcccgggact ccgggctgcc ggcgcgtgtg gtgaagatcg cggtgggctt
     2041 gccgtcccag gcggcggagt acaccacggt ctctccatcg ggggcatagc gggcggaggt
     2101 gatcatcccg ggccggaagg tcatctgcgt gtacgacgcc tcggtttcgg aggggcgcga
     2161 cgagaggccg gcccagtatc cggccgcggc gagcagcacc gcggcggcga tcgccggcca
     2221 cagcaggacg cgggccccga ggaggccgga gcgtgcgccg ccgaccgcgg tcccggtcga
     2281 cttcccggcg cccgcgatcg cgcccgcgat cgaaccggcc gagatcgccg acaacgcttc
     2341 caactggaag ccgagatcgc gcgccgactg gaagcgctcg tccgggctct tctcgaggca
     2401 gtgcaggacg atccgctcca gccccggcgg caggtcgctc gtcgagcggc cgaggtcggg
     2461 cgggtcctct ttgaggatcg cgttcatcgt ctcgaccgac gacgaggcgc tgaaggcccg
     2521 ccggccggag agcatctcgt agaggatgca gccgaacgag aagatgtccg accggtggtc
     2581 ggcgggcagg ccgcgcacct gctcgggcga catgtagccg accgtgccga ggatgacccc
     2641 ggcgtcggtg ccggcggcgg tcggggcgga ggtgagcgcc tcgggcgcga ccgcgccggc
     2701 ctcggggcgc gtcagcttcg cgaggccgaa gtcgaggatc ttgacgcggc cgtcgttggt
     2761 gacgaagaga ttctcaggct tcaggtcgcg atggacgatc cccttctggt gcgccgcggc
     2821 gaggccgatc gcgatctgac gcgcggagtc gatcgccttg cgcgccggca gcggaccgcc
     2881 cgcgaggcgg gcgcgcagcg tgtcaccctc gagcagctcc tcgaccacgt acggcgtccc
     2941 accgtgcgtg ccgatgtcgt agacggcgac gatgttcggg tgattgagct gtccggtggc
     3001 gcgcgcctcc tgctcgaagc ggcgcaggcg ctcgccgtcg gcggcgaacg acgccggcag
     3061 gaccttgact gccacgtcgc ggtgcaggcg agcgtcgcgg gcccggtaca cctctcccat
     3121 gccgccggcg ccgagaggac cgacgatggt gtacgggccg agcgtcgtgc cgggagggag
     3181 cggcatgcgg tcgggaggat agccgcggcg acggggaggt gtccacttct ggcgcggggc
     3241 cgggacgtcc ggctcgaaag agcctgcccg gtttcatggt tctcaccagc cggcttcccg
     3301 gagacggggg tcgcggacag ctccggtcac gatggccgcc gcggccggga ccggagttcc
     3361 ctgcgcgttg accagccgga agccgccccg atgttgcacg tacacgtgcg tatagcggct
     3421 gcgaacctgg aacgtctgag cgtcgaagcg ccctgtcatc cgggtctgac cggtgacaat
     3481 ggcggtgccg ccatacaggc gcacccgggc gggcatacgc acgatcgatt cgaagaccag
     3541 ccgccgtgag ccgacgagat cgacgaagtc gaggcgcggg acttcggagc cggtcaagac
     3601 gtcgatcagg atgcagtcgt cggccacgat ctccccgagg gcctgtcgat tgccctcgga
     3661 cagcgccgcg aaaaaccgct cttccgcctg caggaccact ttgtcggtgt tcatggaatc
     3721 ctccttgtcg ttgggacccg ggtaaggcaa gcggaatgcc agatcgattc cgcagcgtct
     3781 tcgatggtct caagtgcggc gcgcacgtcg tcatgccgct cctttgggag aatgccaacg
     3841 ggatggcagg cgccgggccc gctctcggat ccatccctct cggatccgct ggttcggttc
     3901 ttgcgaagtg ctcgagccat gtgccgcgcg gcgctgccgc cggcgggaga ttgacgtggt
     3961 atgctcgcca acacccttct tcgagttcgg gctggggctt ccgtgatgac gaagccgttc
     4021 gatgtctccg acgatgaagc ccggctccag gcgctgcggc ggacctcgct gctcgacagt
     4081 ccgccggaag aggccttcga ccgcctgacg cgcacggcga cgaccgtgct ccgcgtgccg
     4141 atggcgatcg tctcgctggt ggatcgcgac cggctgttct tcaagagcca gagcgggctg
     4201 gcggagccgc tcgcctcact gcggcagggc ccgatccagc attcgttctg tcaatacgcg
     4261 gtcggtaccc gggaaccgct cgtgg
//
LOCUS       DASRIQ010000204         4609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2414982, whole genome shotgun sequence.
ACCESSION   DASRIQ010000204 DASRIQ010000000
VERSION     DASRIQ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414982"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1956
                     /locus_tag="VFD06_05055"
     CDS             <1..1956
                     /locus_tag="VFD06_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZN02935.1"
                     /translation="VNEIRALRSFQDAEQASRRGQRLFAALEALPFPVVAAIGGPCLG
                     GGTELALACHYRVAADQPRLEIGLPEVRLGILPGWGGTQRLPRLVGLAPALDIILNGR
                     SLDARRARAVGLVDDVAPPELLLHAAERLIEEAHDGTRSRAGRLSDGLLARVNPWRRA
                     SIALTARVARQRLQSKVNERHYPAPYLALEAVTHGLVAGREEGFAFEAESLGHLAVGP
                     TSRGLVALFALQQDARRDPVVAARPRPIESTAVVGAGVMGGGIARALARAGFTVRLKD
                     IDVQALGRGMAVAHGLEGADLRKRRITRGERDRRVDRLLPTLEYTGFKRATVVIEAVV
                     ESLRVKHQVLRDLEAAAAEDFIFATNTSSIPIAAIASPARRPDRVIGMHFFNPVHRMP
                     LVEVIRGPQTSDETVATVVDLTRRIGKTPVVVGDAPGFLVNRILMTYLGEALLLFEEG
                     ARVDAVDRAMREFGMPMGPFALLDQIGIDVAAHVAQVLTEAFPDRAPKTGALHAMKER
                     GWLGRKAGKGFYLYREGARDEPDATSSAPGEVHAEAASVVPSARTAAAAPGALESRLV
                     LPMINEATRCLESRLVRDPAAIDLAMVLGAGFPPFRGGLLRHADSLGLPTVVQSLDLL
                     AQRLGTRFQPARLLADLARSGRGFFAE"
     gene            1970..3691
                     /locus_tag="VFD06_05060"
     CDS             1970..3691
                     /locus_tag="VFD06_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN02936.1"
                     /translation="MAAPKRSFMKSLFAGRLDAGLLFPYPQQDDEGRETLNLLLETFR
                     GWAGDNLDGAAIDRRGAFPEEEVRALKEMGFFGMTIPEAYGGSGLPLTSYCRLMEEIC
                     RHCATTATIVGAHLGIGSKGLVLYGNEDQKRKWLPSVARGDLLCAYALTEPGSGSDAA
                     SLTTRAVWDGKRNVWRLSGGKRYITNGGHAGLFTVFARMPVNGADKISAFIVTRDLAG
                     VTTGKEEEKLGLKGSSTTDLNLQDVAVPEGNLLGEAGRGFKYAMEILNDGRVSLAAGS
                     VGVAKEMTDRAVAYALERRQFGKPIAEFEMIRAKVAEMATGTYAAESMVYLTTALASD
                     KSIDASIESALCKVFASENAWRVVNHAVQIAGGNGFIKEYPYERALRDCRINMIFEGT
                     NEILRLFVALSAVQNLGDELKKVATALQHPIGQIGVLSEFALRKARMALTDQGFPGID
                     PALAKTAARAAHYAEALSASAEAALRLHRKGFIEREYLHERLSEAAGDLYALIACLSR
                     ADTRIKKEGIEAARRDILMTRTFGNAAWRRIRRLLHQVGKNQDANLTRVSDLAYESRG
                     FGPDLVG"
     gene            3714..>4609
                     /locus_tag="VFD06_05065"
     CDS             3714..>4609
                     /locus_tag="VFD06_05065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN02937.1"
                     /translation="MAESVGLTCSSCGEALAAVSRYCPGCGGVVDVSAGSPTRTLHGH
                     GAGSAAATTPDRSGRATATSSSPSSDSDHGRFVPGTLLLGRYRVLGLLGRGGMGEVYR
                     ADDLKLGQTVALKFLPADLAQDAGRLARFHAEVRLARQVSHPSVCRVYDLGDLDGQPF
                     LSMEYIDGENLSTLLRRIGRLPEDKATEVARQICAGLAAAHERGVVHRDLKPGNVMID
                     GRGRARIADFGLAGAIGAEDGSRSGTPAYMAPEQLRGETGVASDLYALGLVLYELYTG
                     KRAFRGETMAEIARSRDEAPPSP"
BASE COUNT          621 a         1654 c         1693 g          641 t
ORIGIN      
        1 gtcaacgaga tccgcgccct gcgatccttc caggacgccg agcaggcgtc gcggcgcggc
       61 cagcggctgt tcgcggcgct cgaggcgctc ccgttcccgg tggtcgccgc gatcggcggt
      121 ccgtgcctcg gcggcggcac cgagctggcg ctggcctgcc actaccgcgt cgcggcggac
      181 cagccgcgcc tggagatcgg gcttccggag gtgcggctcg gcatcctccc gggatggggc
      241 gggacgcagc ggctgccgcg tctcgtcgga ctggcgccgg cgctcgacat catcctgaac
      301 ggccgctcgc tcgacgcgcg ccgcgcccgc gccgtcggcc tggtcgacga cgtcgccccc
      361 cccgagctgc tgctgcacgc cgcggagcgg ctgatcgagg aggcgcacga cggcacgcgg
      421 agccgcgccg ggcggctctc cgacgggctt ctggcgcggg tcaacccgtg gcgccgcgcg
      481 tcgatcgcgc tgacggcgcg cgtggcgcgt cagcgcctgc agtcgaaggt gaacgagcgc
      541 cactacccgg ccccgtacct ggcgctcgag gcggtgacgc acggtctcgt cgccggacgg
      601 gaggaggggt tcgccttcga agcggagtcg ctcgggcacc tggcggtcgg gccgacgagc
      661 cggggactcg tcgcgctgtt cgcgctgcag caggacgcgc gccgcgatcc ggtggtggcg
      721 gcccgcccgc gtccgatcga gtcgaccgca gtcgtcggcg ccggcgtgat ggggggcggc
      781 atcgcccgcg ccctggcccg cgccgggttc acggtgcggc tcaaggacat cgacgtgcag
      841 gcgctcggcc gcggcatggc ggtggcgcac ggcctcgagg gggcggatct gcgcaagcgc
      901 cgaatcaccc ggggcgagcg ggaccgccgg gtcgaccggc tgctgccgac gctcgagtac
      961 accgggttca agcgcgcgac ggtggtgatc gaggcggtcg tcgaatccct gcgcgtcaag
     1021 caccaggtgc tgcgcgacct cgaggcggcg gccgcggagg acttcatctt cgcgaccaac
     1081 acctcgtcga tcccgatcgc cgccatcgcc tcgcccgcgc gccggcccga ccgggtgatc
     1141 ggcatgcact tcttcaaccc ggtgcaccgc atgccgctcg tcgaggtgat ccgcggcccg
     1201 cagacctccg acgagacggt cgccacggtg gtcgacctga cccgccgcat cggcaagacg
     1261 ccggtggtcg tcggcgacgc ccccgggttc ctggtgaacc gcatcctcat gacgtacctg
     1321 ggggaggcgc tcctcctgtt cgaggagggg gcgcgggtgg acgccgtcga tcgcgccatg
     1381 cgggagttcg gcatgccgat ggggccgttc gccctgctcg atcagatcgg gatcgacgtc
     1441 gcggcgcacg tggcgcaggt cctgaccgaa gcgtttcccg accgggcgcc gaagaccggc
     1501 gcgctgcatg cgatgaagga gagaggctgg ctcggccgca aggccggcaa ggggttctat
     1561 ctctaccgcg agggggcgcg ggacgagccg gatgcaacct cctccgcccc gggcgaggtg
     1621 cacgcggagg ccgcctccgt cgtcccgtcg gcgcgcaccg ccgcggccgc gccgggggcg
     1681 ctcgagtcgc gcctggtgct cccgatgatc aacgaggcga cccgctgcct cgagtcgcgc
     1741 ctggtgcgcg acccggcggc gatcgatctc gccatggtcc tcggcgccgg cttcccgccg
     1801 ttccgcggcg gactcctgcg tcacgccgat tccctcgggc tgccgacggt ggtccagagc
     1861 ctcgatctgc tcgcccagag gctcgggacc cgcttccagc cggcgcgcct cctcgcggac
     1921 ctcgcccgct ccgggcgtgg cttcttcgcc gagtgacgag gaactcctga tggccgcgcc
     1981 gaagcgctcc ttcatgaagt cgctgttcgc cggccgtctg gacgccgggc tcctctttcc
     2041 ctacccgcag caggacgacg aggggcgcga gaccctgaac ctcctgctcg agacgttccg
     2101 cggctgggcc ggggacaacc tcgacggcgc cgccatcgat cggcgcggcg ccttcccgga
     2161 ggaggaggtg cgggcgctca aggagatggg gttcttcggg atgaccatcc cggaggccta
     2221 cggcggctcc gggctcccgc tcacctccta ctgccgcctg atggaggaga tctgccggca
     2281 ctgtgccacg accgcgacca tcgtcggggc gcatctcggg atcggcagca aggggctggt
     2341 gctgtacggc aacgaggatc agaagcggaa gtggctcccg tccgtggcgc gcggcgacct
     2401 gctgtgcgcg tacgcgctca ccgagcccgg ctcgggatcg gacgcggcgt cgctgacgac
     2461 gcgcgccgtc tgggacggca aacggaacgt ctggcgcctc tcggggggca agcgctacat
     2521 caccaacggc ggccacgcgg ggctgttcac cgtcttcgcg cgcatgccgg tcaatggcgc
     2581 ggacaagatc agcgccttca tcgtgacgcg cgatctggcc ggggtcacga ccggcaagga
     2641 ggaggagaag ctcggcctca agggctcctc cacgaccgac ctgaacctgc aggacgtcgc
     2701 cgtccccgag gggaacctcc tcggcgaggc ggggcgcggc ttcaagtacg ccatggagat
     2761 cctcaacgac gggcgcgtct cgctcgccgc cggctcggtc ggcgtcgcca aggagatgac
     2821 cgatcgggcc gtggcctacg cgctcgagcg ccgccagttc ggaaagccga tcgccgagtt
     2881 cgagatgatc cgcgccaagg tcgccgagat ggcgaccggc acctacgccg ccgagagcat
     2941 ggtgtatctg acgaccgcgc tcgcctccga caagtcgatc gacgcctcca tcgagtcggc
     3001 gttgtgcaag gtgttcgcgt cggagaacgc ctggcgcgtg gtcaaccacg cggtgcagat
     3061 cgccggcggc aacggcttca tcaaggagta cccgtacgag cgggcgctgc gcgactgccg
     3121 gatcaacatg atcttcgagg ggacgaacga gatcctgcgc ctgttcgtcg cgctgtcggc
     3181 ggtgcagaac ctcggcgacg aactcaagaa ggtggcgacc gccctgcagc acccgatcgg
     3241 gcagatcggc gtgctgtcgg agttcgcgct gcgcaaggcg cgcatggcgc tcaccgacca
     3301 ggggttcccc ggcatcgatc cggcgctcgc gaagaccgcg gcgcgcgccg cgcactacgc
     3361 cgaggcgctg tctgcgtccg ccgaggcggc gctccggctg catcgcaagg ggttcatcga
     3421 gcgggagtac ctgcacgagc ggctcagcga ggcggccggg gacctctacg ccctgatcgc
     3481 ctgcctgtcg cgcgccgaca cgcggatcaa gaaggaaggg atcgaggcgg cgcggcgcga
     3541 tatcctgatg acgcgcacgt tcggcaacgc ggcctggcgg cgcatccggc gcctcctgca
     3601 ccaggtgggg aagaaccagg acgccaacct gacgcgcgtc agcgatctcg cctacgagag
     3661 ccgggggttc ggccccgacc tcgtcggctg aggcgaatgc accggagtgt ccgatggctg
     3721 agagcgtcgg gctgacctgc tcctcctgcg gcgaggcgct cgccgccgta tcccgctact
     3781 gccccggctg cggcggcgtg gtcgacgtgt ccgccgggtc tccgacccgc acgctgcacg
     3841 gccacggcgc cggctccgcg gcggccacga ccccggatcg atccggtcgc gcgacggcga
     3901 cctcctcctc cccctcctcg gactccgacc acgggcgctt cgtcccgggg acgctcctcc
     3961 tggggcgcta ccgggtcctc gggctgctcg gccggggcgg catgggggag gtgtaccggg
     4021 ccgacgacct caagctcggc cagaccgtgg cgctgaagtt cctgccggcc gatctggcgc
     4081 aggacgccgg gcgtctcgcc cgcttccacg ccgaggtgcg gctggcccgg caggtgtcgc
     4141 acccgagcgt ctgccgcgtc tacgacctcg gcgatctcga cggccagccg ttcctgtcga
     4201 tggagtacat cgacggcgag aacctgtcga cgctcctgcg gcggatcggc cggctgccgg
     4261 aggacaaggc gaccgaggtg gcgcggcaga tctgcgccgg cctggcggcg gcgcacgagc
     4321 gcggcgtcgt gcatcgggat ctcaagccgg ggaacgtcat gatcgacggg cgcggccgcg
     4381 cccgcatcgc cgacttcggc ctcgcggggg cgatcggcgc ggaggacgga tcgcgctccg
     4441 gcaccccggc ctacatggcg ccggagcagc tgcgcggcga gactggggtg gcgagcgacc
     4501 tctacgccct cggtctcgtg ctgtacgagc tatacacggg gaagcgcgcc ttccgggggg
     4561 agacgatggc ggagatcgcc cgctcgcgcg acgaggcccc gccgtcccc
//
LOCUS       DASRIQ010000205         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_608125, whole genome shotgun sequence.
ACCESSION   DASRIQ010000205 DASRIQ010000000
VERSION     DASRIQ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608125"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2212)
                     /locus_tag="VFD06_05070"
     CDS             complement(<1..>2212)
                     /locus_tag="VFD06_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012142094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN02938.1"
                     /translation="TGLALPVLHAGDNVLVVGVWNSGAPTSTELVVVPFLSAANTSAV
                     TRGPWLQMGTPTSLVVRWRTDVATGGRVLYGTDPGDLSSTVLDATPTTEHALALTGLT
                     PSTRYYYAVGSPTAILAGGDASYSFQTPPTTGTAAPTRVWVVGDSGTADANARAVRDA
                     YTQYSAGASTQLWLMLGDNAYPEGTDPQYQSAMFDMFPEMLRQSVLWPTLGNHDGITA
                     DSATQTGPYYDNFTLPTAAQAGGLASGTEAYYSFDHANIHFICLDSFETSRATGGAML
                     TWLQQDALSTNQPWIIAFWHHPPYSKGSHNSDTEIELIEMRQNALPILEQAGVDLVLA
                     GHSHSYERSFLIDGHYGFSSTFGPSMKVDGGDGRIGGQGAYSKPSAGPAAHEGAVYIV
                     AGTSGQVSGGTLNHPAMFLSLNTLGSLVLDVNDRRLDAGFLDSTGVLRDDFTMIKGVT
                     APAADFDATPTSGTAPLLVQFTDLSADGPTAWSWDFDDNGSVDSLERSPAYTYASPGL
                     YTVRLTASNVAGPSAQASPDLICVTSADGTADADGDGTPDGVDVCPCVPDPGQEDADQ
                     DGSGDACDGDDDNDGVADAADCAPLLRGVSSPPDPIADTLRLQQAGTPPETLLHWTRS
                     RGGHTANVYRGTLPLSGPRSRNETCMQTEVTATEIADPEAPPPGFFFYYLVSARNTCA
                     ESAAGRDSAGQDIIPALPCGMLNRDSDGDGIPDAGDTCPLAADASQYDADSDTVG"
BASE COUNT          321 a          713 c          821 g          357 t
ORIGIN      
        1 cccaccgtgt cggaatcggc gtcgtactgg gaagcgtcgg cggcaagggg acaggtgtcg
       61 cccgcgtcgg gaatcccgtc gccgtcgctg tcccggttca gcatcccgca ggggagcgcg
      121 ggaatgatgt cctggcctgc actgtcccgg cctgccgcgc tctcggcgca ggtgttgcgc
      181 gcgctcacca ggtagtagaa gaagaagccg ggcggagggg cttccgggtc cgcgatctcg
      241 gttgccgtga cttcggtctg catgcaggtc tcgttgcggg agcgcgggcc gctcagcggg
      301 agcgtgccgc ggtacacatt ggcggtgtgt ccgccgcgac tgcgcgtcca gtgcagcagc
      361 gtctcggggg gcgtgccggc ctgctgcagc cgcagagtgt cggcgatcgg gtccggcggc
      421 gaggacactc cgcgcagcag gggagcgcag tccgccgcat cggcgacgcc gtcgttgtcg
      481 tcgtcgccgt cgcacgcatc gccacttccg tcctggtcgg cgtcctcctg cccgggatcg
      541 gggacgcagg gacagacgtc gacgccgtcc ggggttccgt cgccgtccgc gtccgcggtg
      601 ccatcggcgg acgtcacgca tatcaggtcg ggactcgcct gcgcgctcgg tccggcgacg
      661 ttcgaggcgg tgaggcgcac ggtgtacagc cccggagacg cgtaggtgta ggcggggctc
      721 cgctccaggc tgtcgaccga cccgttgtcg tcgaagtccc aggaccacgc ggtcggtcca
      781 tcggcggaga gatcggtgaa ctgcaccagt agcggcgcgg tgcccgaggt cggggtcgcg
      841 tcgaaatcgg cggccggcgc cgtcacaccc ttgatcatcg tgaagtcatc ccgcaacact
      901 cccgtggagt ccaggaaccc ggcgtcgagc cggcgatcgt tgacgtccag gaccagggac
      961 ccgagggtgt tgagcgacag gaacatggcc gggtggttca gggtgccgcc gctgacctgg
     1021 ccggaggtgc cggccacgat gtagaccgcg ccctcgtgcg ccgccggccc ggcggacggc
     1081 ttgctgtagg ccccctgtcc gccgattcgg ccgtccccgc cgtccacctt catcgagggg
     1141 ccgaaggtcg aggagaaccc atagtggcca tcgatgagga acgagcgctc gtacgagtga
     1201 ctgtgcccgg caagcaccag gtcgacgccc gcctgttcga ggatcggcag ggcattctgg
     1261 cgcatttcga tgagctcaat ctcagtgtcc gagttgtgcg atcccttgct gtagggcgga
     1321 tggtgccaga aagcgatgat ccacggctga ttggtggaca gcgcatcctg ctgcagccag
     1381 gtcagcatgg cgccgcccgt ggcccggctg gtctcgaacg agtcgaggca gatgaagtga
     1441 atgttggcgt gatcgaacga gtagtaggct tccgtgccgg aagcaagtcc gcccgcctgc
     1501 gccgcggtcg gcagggtgaa gttgtcgtag taggggccgg tctgggtcgc cgagtcggcg
     1561 gtgatcccgt cgtggttgcc gagcgtcggc cagaggacgg actggcgcag catctcgggg
     1621 aacatgtcga acatggccga ctggtactgc ggatcggtcc cctcggggta ggcattgtcc
     1681 cccagcatca accacagctg tgttgatgcg ccggcgctgt actgcgtgta ggcgtcccgc
     1741 accgcgcggg cgttggcgtc cgccgtgccg gaatcgccga cgacccagac gcgcgtcggc
     1801 gcggcggtgc cggtcgtcgg cggggtttga aacgagtagg acgcatctcc tcccgcgagg
     1861 atcgcggtgg gactgccgac ggcatagtag tagcgggtcg acggagtgag cccggtcagg
     1921 gccagcgcgt gctcggtcgt cggcgtcgcg tcgagcaccg tcgagctcag gtcgccaggg
     1981 tcggtcccgt acaggacgcg gcccccggtc gccacgtcgg tgcgccagcg cacgacgagg
     2041 ctcgtcggcg tgcccatctg aagccagggt ccccgtgtca cggcactcgt gttggcggcg
     2101 gacaggaaag gcacgaccac cagctcggtg gacgtcgggg cgccgctgtt ccacaccccg
     2161 accacgagga cgttgtcacc ggcgtgcagg accggcagcg ccagcccggt ga
//
LOCUS       DASRIQ010000206         8999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3748396, whole genome shotgun sequence.
ACCESSION   DASRIQ010000206 DASRIQ010000000
VERSION     DASRIQ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3748396"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFD06_05075"
     CDS             complement(<1..711)
                     /locus_tag="VFD06_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZN02939.1"
                     /translation="MTPRSALLPTVFILLAAVVLTAGSAPPPAGAATASSSSGPAPGI
                     AMREVRIPMPDGVRLAADLWMPADAAAKKPKRYPVLLEYLPYRKTESRGDRFGLYAYF
                     VRRGYVVARVDIRGTGNSEGVLIPHEYSDIENQDGDAAIAWLARQAFSTGKVGMFGIS
                     WGGFNSIHMAMRNPPALGAIIAVDATDDLYQDDVHFMDGGIHVDSWEMSMDLANAMPG
                     APDYAIDDRLFADRFDQKP"
     gene            complement(757..3147)
                     /locus_tag="VFD06_05080"
     CDS             complement(757..3147)
                     /locus_tag="VFD06_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN02940.1"
                     /translation="MTRGRFALLGAALAVALLVAVLWARSKGPAAPAHGDPPLSLLLV
                     TIDTLRADHLGCYGDGDASTPAIDRLAAEGVLFEQASTCVPVTLPSHASILTGTYPVF
                     HGVRNNGAYRLDDAALTLAEILRGRGWRTGAVIAAYPLLARFGLAQGFDTYDDALPPE
                     RERQIGYREKSAEEVSRAGLDFLAKDAEPPFFLWLHYFDPHIPYAPPEPFAGRFRGKP
                     YDGEIAFVDEQIGRVLERIRRPDLASRTLVVLMSDHGEGLGDHGEWTHGVFLYESTLR
                     VPLMMALPGVLPAGRRVATPVSSIDVLPTVLDLLRVPVPDEVQGESLRPLFEGSAAAP
                     RVILAESEMPRENYGWSGLAAIREGGLKLIRAPRRELYDLSADPREAEDRSSARPEEA
                     ARLAADLDRRLTEATRAALDGSGRQEPDEETRKRLASLGYILSPGPARRAPLPDPKDR
                     IAVLDLLDQGRVLAADGKLAEAIATYTRALGADPGNPTVYERRGNARLASKDFRGAQK
                     DYAKVLGSHPDHLEALQNMGSALLGQGDLEGAADHFSRVLALAPDSPRTLTSLGIVRY
                     RQGRPDEARPLFKRALALEPDSPQAKEWAAAAGEGPAAESPGGTPTGRAPIDQARSLY
                     EAGRFQEIVTLADGEIRDGRDAPDLRFVRGNALFRLGKVEDAAADYRRVLEARPDDVG
                     ALFGLALVDLRQGKTAAAMRRLEQAKRIDPRHPDVARNLAILYDEAGRTGDAITSYES
                     ALALAPDALELRFFLGRALAKAGRKSEARSQFQAYLDGGGTEYRDAARSALAGLKP"
     gene            complement(3165..4655)
                     /locus_tag="VFD06_05085"
     CDS             complement(3165..4655)
                     /locus_tag="VFD06_05085"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZN02941.1"
                     /translation="MAARPKVSMLSPEEVRLIHESSLEILEHTGVLYENKAALDVLEA
                     HGCKVDRDRGIAYIKPDLVEKCIKSAPRHFVLASRDGKNDAVIDGKKMHHMTDGQASF
                     TLDEKTGERRTSTLADLTLSTLLADALDPVKVMWSTVFPTDASTDKRALFETAASFIW
                     SSKHFQMVGGVQDPDDVPYLLAMIDAVSGDRRKQRDRPIFSMVTCPVSPLKHDDAMTE
                     ACIKLAKEWVPIVFWPMPLQGATAPITMAGTLLVTNTEFLSGLVLYQCVQPGLPMIYP
                     AGPETLDMKTGLASTASPEGILLNLPFAQMTEFYGVPYMAGGICSDAKIPGIQAGYEN
                     MATGMTAALSGCDLLVGIGILEDGNTLSFPQMVIDEEICNILSFVRDGMEVTRETLMV
                     ESIQKVGHSPNHHLGEPFTRHFLKSGQGYWPRISFRGTHGDWKRRGKDEVQLARDRMK
                     ALLDKHEPAPLAPEVLKELRRLLVERTEFSENDPRIEGVFRHPWKG"
     gene            4782..5240
                     /locus_tag="VFD06_05090"
     CDS             4782..5240
                     /locus_tag="VFD06_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02942.1"
                     /translation="MLLRLAVPSAATGKAGSRRAPRGSTARQVRGACENAPVIDHLSP
                     REMETLEAAAASGESWPIVLRRFFVDGTRLVLGWDQAWSEPEMLAALASDPAGGGRAP
                     SGAIDRAGLRAALERAARFCTEHGLHLLSFEGEGPGEEPLGERRWFLAVG"
     gene            5338..6927
                     /locus_tag="VFD06_05095"
     CDS             5338..6927
                     /locus_tag="VFD06_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN02943.1"
                     /translation="MKRDVVVIGAGHNGLVCAALLAEAGHRVTIVEARGLPGGACVSE
                     ELWPGYQVSTTSYVSTLLLPQVVQRFDLPRHGYKVVKQEPAFFVPFPDGRTLTLWGDD
                     RDLAEIAKFSKRDAEAYPRFHHALARVGAFLKPQILKPPPPLAPGGPRDLFDLLRLAR
                     DARKLTPLETQFMVALATRGVAEIVDSWFESEEIKAFLCSQAVIGAYGGVHQPGTAFL
                     LLHDVLGGVDGAAGVWGVVVGGMGGITRALASAAQERGATLRLSAPVEGIDLNDRGEV
                     AGVRLSGGETLHASVVVSNAAPRVTYLKLLPKGALPADLTAHMESFQDLGASLKVHLA
                     LSELPDFTAAPGKTAGPQHRGLLNFCPTVDFIERAWDDCKRGGFSERPTIEACLHSVN
                     DPSCAPPGKHVMTCFVQYGPRHLKEGRWEDLKETVADRVIAELARYAPNLPGAVLHRH
                     VYTPEDLEKVFGLPGGNIYHGAMTPDQLFSFRPAAGWADYRTPVRRLYLCGSGTHPGG
                     GVWGAAGWNAGHQVLRDIGRG"
     gene            complement(6966..8147)
                     /locus_tag="VFD06_05100"
     CDS             complement(6966..8147)
                     /locus_tag="VFD06_05100"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02944.1"
                     /translation="MKLGERFIQQGLLTPAQLEAALKAQLIFGGHLGTVLLEFGHVDE
                     HTLGKTLGKIFNVDYAPPGLFTDIPKTILDLVPKRLVEKHHAVPFDKRDRHLAVAMID
                     PKDLQAIDEISFATGHTIHPWVAPEARIFQVMERYYDLPRRHRYISVCQTLDAAGAAA
                     AKGPGGPSERERAVLDSAYAPPPYLAALGSSPPDAAADAEGPPPAPLSITGIDGEAGP
                     GTETQLSDSLCRSEGASEIAEAAVQFLSSRLKRSILFLVRGDTATVWRTHGPAQSTPT
                     ALLPLTSEPLFALLHERDHYQGPLPAHPRIEQMMATFGMKPPREIVLLPGYLDDRLLV
                     IVYGDSGPDAAIAAEVGDLRPIVTKIACALHLVALKRRIRSTAEAAEAAAPSQLAAKA
                     A"
     gene            8279..8656
                     /locus_tag="VFD06_05105"
     CDS             8279..8656
                     /locus_tag="VFD06_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02945.1"
                     /translation="MATQDSCCTIVPYFKVHAGKMPMFRDLCERFVARTSTEPKCLYY
                     GFSFDGDEVHCREGYVDAEGALQHLENVGALIKESLTIADLVRLEIHGPASELEKLRA
                     PLEPLQAKFFRLEYGFRRLECSR"
     gene            complement(8658..>8999)
                     /locus_tag="VFD06_05110"
     CDS             complement(8658..>8999)
                     /locus_tag="VFD06_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZN02946.1"
                     /translation="TAAPMSRSLAAGSPQAFPEWKASFVDGAGGQSVFPRMWERMKPL
                     VDDSIVVTLDQTKAAMRLMAEKARVIAEGAGALPLAAALTGKAGRGPIVAIVSGGNID
                     LAKFAQLVGAV"
BASE COUNT         1297 a         3004 c         3276 g         1422 t
ORIGIN      
        1 cggcttctgg tcgaagcgat cggcgaagag ccgatcgtcg atggcatagt ccggggcgcc
       61 gggcatggcg ttggcgaggt ccatgctcat ctcccaggaa tcgacgtgga tgccgccgtc
      121 catgaagtgg acgtcgtcct ggtagaggtc gtcggtggcg tccacggcga tgatggcgcc
      181 gagcgccggc gggttgcgca tcgccatgtg gatcgagttg aacccgcccc acgagatgcc
      241 gaacataccg accttcccgg tcgagaacgc ttgccgcgcg agccaggcga tcgccgcgtc
      301 gccgtcctgg ttctcgatgt cgctgtactc gtgcgggatg agcacccctt cgctgttgcc
      361 ggtgccgcgg atgtcgacgc gcgccacgac gtacccgcgc cgcacgaagt aggcgtagag
      421 cccgaaccga tcgccgcggc tctccgtctt gcgatacggc aggtattcga ggaggaccgg
      481 gtaacgcttg ggtttcttcg cggcggcgtc cgccggcatc cagagatcgg cggcgagcct
      541 cacgccgtcc ggcatcggga tccgcacttc gcgcatcgcg atcccgggcg cggggccgct
      601 ggaagagctc gcggtcgcgg cgccggcggg cggcggcgcg gagccggccg tcaggacgac
      661 ggccgccagg aggatgaaga cggtcggcag gagcgctgaa cgcggtgtca tgtcattccg
      721 gcttccgcag ggcggcgcgg attctgacac actcgatcaa ggcttcaggc cggcgagggc
      781 ggagcgggcg gcgtcacggt actcggtgcc gccgccatcg aggtaggcct ggaactggga
      841 gcgggcctcg ctcttgcggc ccgccttcgc cagcgcacgg ccgagaaaga agcgcagctc
      901 gagagcgtcc ggggccaggg cgagggccga ctcgtacgag gtgatcgcgt ccccggtccg
      961 ccccgcctcg tcatagagga tggcgaggtt ccgcgcgacg tcgggatggc gcgggtcgat
     1021 ccgcttcgcc tgctcgagcc ggcgcatcgc cgcggcggtt ttcccttgcc gcaggtcgac
     1081 gagggccagc ccgaagaggg cgccgacgtc gtccggacgc gcctcgagga cgcggcggta
     1141 gtcggcggcg gcgtcctcca ccttgccgag ccggaacagg gcgttgccgc ggacgaagcg
     1201 gagatcgggc gcgtcgcgtc cgtcgcgaat ctccccgtcg gcgagcgtga cgatctcctg
     1261 gaaccgtccg gcctcgtaga gcgaccgcgc ctggtcgatc ggtgcccgcc ctgtcggcgt
     1321 cccgccgggg gactcggcgg cgggcccctc tccggccgcc gccgcccact ccttcgcctg
     1381 cggcgagtcc ggctcgaggg ccagcgcccg cttgaagagc ggcctggcct cgtccggacg
     1441 cccttgccgg tagcgcacga tgccgaggga ggtcagggtc cggggggaat cgggggcgag
     1501 cgcgaggacg cgggagaagt ggtccgcggc gccttcgagg tccccctggc cgagaagggc
     1561 gctccccatg ttctgcagcg cctcgagatg atccggatgg gagccgagga ccttcgcata
     1621 gtccttctgg gcgccgcgga aatccttcga ggcgagccgg gcgttgccgc gccgctcgta
     1681 caccgtcggg ttgcccgggt cggctccgag ggcgcgcgtg taggtcgcga tggcctccgc
     1741 gagctttccg tcggcggcga ggacccgccc ctggtcgaga aggtcgagga ccgcgattcg
     1801 atccttcggg tcggggagag gtgcccggcg cgccggaccg ggagagagga tgtaaccgag
     1861 cgacgccaga cgtttccggg tctcttcgtc cggctcctgg cgcccgcttc catccagagc
     1921 ggcccgggtc gcctcggtca gccggcgatc gaggtcggcc gcgagacggg ccgcctcctc
     1981 aggccgcgcc gacgaccgat cctccgcctc gcgcgggtcg gccgagaggt cgtacagctc
     2041 gcggcgcggc gcccggatga gcttgagccc cccctcccgg atggcggcga gtccgctcca
     2101 gccgtagttc tcgcgcggca tctcggattc ggccaggatg actcgcggcg ccgcggcgct
     2161 cccttcgaag agcggccgca ggctctcccc ctgcacctca tccgggaccg gcacgcggag
     2221 cagatcgagg acggtgggca ggacatcgat gctcgagacc ggcgtcgcga cccggcgccc
     2281 ggctggcaga acgccgggaa gcgccatcat gagaggcacg cgcagcgtcg actcgtagag
     2341 gaagacgccg tgcgtccact cgccgtgatc gccgagccct tcgccgtgat ccgacatcag
     2401 gacgaccagc gtcctcgagg cgaggtcggg gcggcggatc cgctccagca cccggccgat
     2461 ctgctcgtcg acgaatgcga tctcgccgtc gtacggcttg ccgcggaacc ggccggcgaa
     2521 cggttccggc ggggcgtagg ggatgtgcgg atcgaagtag tggagccaca ggaagaaggg
     2581 cggttccgcg tccttcgcga ggaagtcgag gccggcgcgg ctgacctcct cggccgattt
     2641 ttcccgatag ccgatctgcc gctccctctc cggcggcagg gcgtcgtcgt aggtgtcgaa
     2701 accttgcgcg agcccgaacc gggcgaggag cgggtatgcg gcgatcacgg cgccggtgcg
     2761 ccagccgcgc ccgcgcagga tctccgccag cgtcagggcg gcgtcgtcga gccggtacgc
     2821 gccgttgtta cgcacaccgt ggaagacggg gtaggtgccg gtcaggatcg aggcgtgcga
     2881 cggcagcgtg accggcacgc aggtcgaggc ctgctcgaac agcaccccct cggccgcgag
     2941 gcgatcgatc gccggagtgc tcgcgtcacc gtccccgtag cagccgaggt gatcggcgcg
     3001 cagcgtgtcg atggtgacga ggaggaggct gagcggcggg tcgccgtgcg ccggtgccgc
     3061 cggtcccttc gatcgcgccc agaggaccgc gacgaggagc gcaaccgcca gcgccgcgcc
     3121 caggagcgcg aaccgacccc gcgtcatacc gcaatcttag cgggctagcc cttccagggg
     3181 tggcggaaga cgccttcgat gcgagggtcg ttctcggaga actcggtccg ctcgacgagg
     3241 aggcggcgca gctccttgag cacctcgggc gcgagcggcg ccggctcgtg cttgtcgagg
     3301 agcgccttca tccgatcgcg cgccagctgc acctcgtcct tgccgcggcg cttccagtcg
     3361 ccgtgcgtgc cgcggaagga gatgcgcggc cagtacccct gtcccgactt gaggaaatgg
     3421 cgggtgaacg gctcgccgag gtggtggttg ggggaatggc cgaccttctg gatcgactcc
     3481 accatcagcg tctcgcgcgt cacctccatc ccgtcgcgca cgaacgacag gatgttgcag
     3541 atctcctcgt cgatcaccat ctgcgggaac gacagggtgt tgccgtcctc gaggatgccg
     3601 atgccgacga gcaggtcgca gccggagaga gccgccgtca taccggtcgc catgttctcg
     3661 tagccggcct ggatgccggg gatcttggcg tcggagcaga tgccccccgc catgtacggc
     3721 acgccgtaga actcggtcat ctgggcgaac ggcaggttga gcaggatccc ctcgggcgac
     3781 gcggtcgagg cgagcccggt cttcatgtcg agcgtctcgg gcccggccgg gtagatcatc
     3841 ggcagccccg gctggacgca ctggtagagg accagcccgc tcaggaactc ggtgttggtc
     3901 acgaggagcg tcccggccat ggtgatcggc gccgtggcac cttgaagggg catcggccag
     3961 aagacgatcg gcacccactc cttcgccagc ttgatgcacg cctcggtcat ggcgtcgtcg
     4021 tgcttcagcg gcgacaccgg gcaggtgacc atggagaaaa tcggccggtc gcgctgcttc
     4081 cgccggtcgc cgctcacggc gtcgatcatc gccaggaggt agggcacgtc gtccgggtcc
     4141 tgcacgccgc cgaccatctg gaagtgcttc gacgaccaga tgaaggacgc ggcggtctcg
     4201 aagagcgcgc gcttgtcggt cgaggcgtcg gtcgggaaga cggtggacca catcaccttc
     4261 accgggtcga gcgcgtcggc caggagcgtc gagagcgtca gatccgcgag ggtcgaggtg
     4321 cggcgctcgc cggtcttctc gtcgagggtg aacgacgcct gcccgtcggt catgtggtgc
     4381 atcttcttgc cgtcgatgac ggcgtcgttc ttcccgtcgc gcgaggcgag gacgaaatgg
     4441 cgcggggccg acttgatgca tttctccacg aggtccggct tgatgtaagc gatgccgcga
     4501 tcgcgatcga ccttgcagcc gtgcgcttcg aggacgtcga gggcggcctt gttctcgtac
     4561 aggacgccgg tgtgctcgag gatctcgagg gacgactcgt ggatgaggcg gacctcttcg
     4621 ggcgagagca tcgagacctt gggccgcgcc gccatgcgag tgcctccttc ctctgcgaga
     4681 ttttcgcagc catgacaaca cgggcgcggc tttcaggtca atcgcgcagc gccataaagc
     4741 gttgctgtga tggcattacg gcatggaggc gtgcggcggc catgctgtta cggcttgccg
     4801 tgccgtccgc tgcgaccgga aaggcgggat cgcggcgcgc gccgcgcgga tcgaccgccc
     4861 ggcaggtgcg gggcgcgtgc gagaatgcgc cggtgatcga tcacctttcg ccgcgtgaga
     4921 tggagacgct cgaggcggcc gccgcgtccg gagagtcctg gccgatcgtg ctgcgccgct
     4981 tcttcgtcga cggcacgcgt ctcgtcctcg gctgggacca ggcgtggagc gagccggaga
     5041 tgctcgcggc gctcgccagc gatcccgctg gcggagggag ggcgccctcc ggggcgatcg
     5101 atcgcgccgg cctgcgcgcc gcactcgagc gcgccgcccg cttctgcacc gagcacgggc
     5161 tgcacctcct gtcattcgag ggagaggggc cgggggagga gccgctcgga gagcggcgct
     5221 ggttcctggc ggtcgggtga ggccgccggg actggctgat acgccacgat ccggccgggt
     5281 gccgggtgcg catggaagag attctcgcgg acgaacattc ggttttgggg acgaagcgtg
     5341 aagcgtgacg tcgtcgtcat cggagcgggg cacaatggcc tcgtatgcgc cgcccttctc
     5401 gccgaggcgg ggcaccgcgt gacgatcgtc gaggcgcggg ggcttcccgg cggggcgtgc
     5461 gtctcggagg agctgtggcc cggctaccag gtctcgacca cctcctacgt ctcgacgctc
     5521 ctcctccctc aagtcgtgca gcgcttcgac ctgcctcggc acggctataa ggtcgtcaag
     5581 caggagccgg ccttcttcgt gccgttcccg gacgggcgca ccctcacgct ttggggggac
     5641 gaccgcgacc tcgccgagat cgcgaagttc tcgaagcgcg acgccgaggc gtacccgcgc
     5701 ttccaccacg cgctggcgcg cgtcggggcg ttcctcaagc cgcagatcct caagccgccg
     5761 ccgccgctcg cccccggcgg accgcgcgac ctgttcgacc tcctgcgcct ggcgcgcgat
     5821 gcgcgcaagc tgacgccgct cgagacgcag ttcatggtgg cgctcgcgac gcgcggcgtc
     5881 gccgagatcg tcgattcctg gttcgagtcg gaggagatca aggcgttcct ctgctcgcag
     5941 gcagtcatcg gcgcctacgg cggcgtgcac cagccgggga ccgcgttcct cctcctgcac
     6001 gacgtgctcg gcggggtgga cggtgccgcc ggggtgtggg gcgtcgtcgt cggtggcatg
     6061 gggggcatca cccgcgccct cgcctctgcg gcgcaggagc gcggcgcgac cctgcgcctc
     6121 agcgcgccgg tcgaagggat cgatctgaat gatcgcggcg aggtggccgg cgtccgcctc
     6181 tctggcggcg agacgctcca cgcctcggtc gtcgtcagca acgcggcgcc gcgtgtcacc
     6241 tacctgaaac ttcttccgaa gggggcgctt cccgccgatc tcacggcaca catggagtcg
     6301 ttccaggacc tcggcgcgtc gctcaaggtc cacctggccc tctccgagct gcccgatttc
     6361 accgccgcgc ctgggaagac ggccgggccg cagcaccgcg ggctgctcaa cttctgcccg
     6421 actgtcgatt tcatcgagcg ggcctgggac gactgcaagc gggggggatt ctcggagcgg
     6481 ccgacgatcg aggcctgcct gcactcggtg aacgatccct cctgcgcgcc ccccgggaag
     6541 cacgtcatga cctgcttcgt ccagtacggc ccgcgccatc tcaaggaggg gcgctgggag
     6601 gacctgaagg agaccgtggc cgaccgggtg atcgcggagc tggcgcgcta cgccccgaac
     6661 ctgccggggg cggtcctgca ccggcacgtc tacacgccgg aggatctgga gaaggtgttc
     6721 gggctgccgg gagggaacat ctaccacggt gcgatgaccc ccgatcagct cttctccttc
     6781 cggccggcgg ccgggtgggc cgactaccgc acgcccgtgc gccggctgta tctgtgcggc
     6841 tcggggacgc atcccggggg aggggtctgg ggggccgccg gatggaacgc cgggcaccag
     6901 gtgctgcgcg acattggtcg cggctgaggc gttgctcagc cttgggtcgc ggctgagcga
     6961 ggcgatcagg ccgctttggc ggcgagttgg gatggcgccg cggcttcggc ggcttcggcg
     7021 gtggagcgaa tgcggcgctt cagcgccacg aggtgcaggg cgcaggcgat cttcgtcacg
     7081 atcgggcgca ggtctccgac ctccgcggca atcgccgcgt ccggcccgct gtcgccgtag
     7141 acgatgacga gcaaccggtc gtcgaggtag cccggcagca ggacgatctc gcgcggcggc
     7201 ttcatgccga acgtcgccat catctgctcg atgcgcgggt gcgcgggcag cggaccctga
     7261 tagtggtcgc gctcgtgcag gagcgcgaac agcggctccg aggtcagcgg caggagcgcc
     7321 gtcggggtgc tctgcgccgg gccgtgcgtc cgccacacgg tcgccgtgtc gccgcgcacc
     7381 aggaagagga tcgaccgctt caatcggctc gagaggaact gcacggccgc ctcggcgatc
     7441 tccgaggccc cctcgctccg gcacagactg tcggagagct gcgtttccgt ccccggaccg
     7501 gcctctccat cgatcccggt gatgctcaag ggcgccgggg gcgggccctc cgcgtcggcg
     7561 gcggcatccg ggggggagga ccccagcgcc gccaggtacg gcggcggcgc ataggcgctg
     7621 tcgagcaccg cacgctcccg ctcgctcgga ccgcccggcc ccttcgcggc agcggccccc
     7681 gcggcgtcga gcgtctggca gaccgagatg tagcggtgcc ggcgcgggag gtcgtagtag
     7741 cgctccatca cctggaagat gcgagcctcg ggggcgaccc aggggtggat cgtgtgcccg
     7801 gtggcgaagg agatctcgtc gatggcctgc aggtccttgg gatcgatcat ggcgaccgcc
     7861 aggtggcggt cccgtttgtc gaacggcacg gcgtggtgct tctccaccag ccgcttcggg
     7921 acgaggtcga ggatcgtctt cgggatgtcg gtgaagaggc ccggcggagc gtagtccacg
     7981 ttgaagatct tcccgagcgt cttgccgagc gtgtgctcgt cgacgtgccc gaactcgagg
     8041 agcacggtgc cgaggtgccc cccgaagatg agctgcgcct tgagcgccgc ctcgagctgc
     8101 gccggggtca gcagcccctg ctggatgaaa cgctctccga gcttcatcga tccggacgga
     8161 atatggggac cggcatcggg ggagtcaatc gtgaccgtgc tccggtcacg cggcatcccg
     8221 ggggcgtcag ccggctgacg ccggtatgat tccgacccag gtgttcgatg gaggtcccat
     8281 ggcgacgcaa gacagctgct gcaccatcgt tccttacttc aaagtccatg ccggcaagat
     8341 gcccatgttt cgggatttgt gtgaacggtt cgtggccaga acgtcgactg agcccaagtg
     8401 tctctattac ggtttctcct tcgacggtga cgaggttcat tgccgcgagg gttatgtcga
     8461 cgcggagggg gcgcttcaac acctggaaaa cgttggagcg ttgatcaaag aatcactgac
     8521 aattgccgat ctcgttcggt tggaaatcca cggacctgca tccgaactcg aaaagcttcg
     8581 cgctccattg gaaccgctcc aggcgaaatt cttcaggctg gagtacggct ttcgacgtct
     8641 ggagtgttct cgctgagtca gacggcgccg acgagctggg cgaacttcgc gaggtcgatg
     8701 ttgccgccgg agacgatcgc cacgatcggg ccgcggccgg ccttgccggt cagcgccgcg
     8761 gcgagcggca gggctcccgc cccctcggcg atgacgcggg ccttctcggc catcaggcgc
     8821 atcgccgcct tcgtctgatc gagcgtcacg acgatcgaat cgtccaccag cggcttcatc
     8881 cgctcccaca tgcgcgggaa gacgctttgg ccgccggcgc cgtccacgaa cgacgccttc
     8941 cactcgggga aggcctgggg cgaacccgcg gccagcgacc gggacatggg ggcggcggt
//
LOCUS       DASRIQ010000207         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1142060, whole genome shotgun sequence.
ACCESSION   DASRIQ010000207 DASRIQ010000000
VERSION     DASRIQ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142060"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VFD06_05115"
     CDS             complement(<1..1121)
                     /locus_tag="VFD06_05115"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZN02947.1"
                     /translation="MDDSPLRDALRAAWKQTAPKPFDPARPVVKLHEATYDDEEIAAV
                     LDCLLHTEVTMGPKVRQFEQEFAKRFGHGHAVMVNSGSSANLLAVAGLANEATANRLQ
                     PGDEVIVPALCWSTTVWPLIQHQLVPVIVDPDPKTLNIDVEQAARAVGPKTRAIMPVP
                     IYGNPCDMDALGALATKHHLTILEDSCESLGATYRGRHVGSFGRAGTFSFYYSHHITT
                     LEGGMVVTDDFELAETLRVLRAHGWTREMEQPKKHLEASPEIHPKFLFVNLGYNLRAT
                     EPQGAMGSVQLRKLDRFIQVRSDNAEHWLRELAGFREFFEFVEVTPGARSSWFGFPMR
                     VKAGAPFTARELMAWFQSRGIEMRPLNAGNIAVQPAIRR"
     gene            complement(1128..2054)
                     /locus_tag="VFD06_05120"
     CDS             complement(1128..2054)
                     /locus_tag="VFD06_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HZN02948.1"
                     /translation="MDPHASVYVAGHRGMVGSALVKRLRAAGHSRLVLRTREELDLTR
                     REAVEEFFLREKPGYVFLAAGRVGGIQANQASPADFIRDNLLIQTSVLHAAHRSGVKR
                     LIFFAGSCLYPRECPQPMKEEAILTGPMEQTSEAYSTSKLAGYMMCEAYNRQYGTQFL
                     TVIPATLYGPGDNFDPATSHVLSALIRRFHEQRRQSSVTVWGTGRPVREFLYVDDLAE
                     ACVLLMDLPGPLPGAPINIGTGRGTSIKELAESIAGLLGYRGSIQFDASKPDGAPIKV
                     LDSSRIHSLGWASRTSLQDGLRRTYEWYLSRN"
BASE COUNT          345 a          699 c          783 g          346 t
ORIGIN      
        1 tggcgccgga tcgccggctg gacggcgatg ttgcccgcgt tgaggggccg catttcgatg
       61 ccgcgcgact ggaaccacgc catgagctcg cgcgccgtga acggggcgcc cgccttcacg
      121 cgcatgggga agccgaacca ggagctccgg gcccccgggg tgacctccac gaactcgaag
      181 aactcccgga agcccgccag ctcgcgcagc cagtgctcgg cgttgtcgga gcggacctgg
      241 atgaaccggt cgagcttgcg gagctggacc gagcccatgg ccccctgggg ttcggtcgcc
      301 cgcaggttgt acccgaggtt gacgaacagg aacttcggat ggatctccgg gctggcctcg
      361 aggtgcttct tcggctgctc catctcccgc gtccagccgt gcgcccggag cacccgcagg
      421 gtctcggcga gctcgaagtc gtcggtgacc accatgccgc cttccagcgt ggtgatgtgg
      481 tgcgagtagt agaagctgaa cgtgcccgcc cgcccgaagg agccgacgtg gcggccgcgg
      541 tacgtggcgc ccagcgactc gcacgagtcc tcgaggatcg tgagatggtg cttcgtcgcc
      601 agcgcgccga gcgcgtccat gtcgcacgga ttgccgtaga tcgggacggg catgattgcc
      661 cgggtcttcg gccccaccgc gcgggccgcc tgctcgacgt cgatgttgag cgtcttcgga
      721 tcgggatcga cgatcaccgg aacgagctgg tgctggatca gcggccagac ggtcgtcgac
      781 cagcagagcg cgggcacgat gacctcgtcg cccggctgga ggcggttcgc cgtcgcctca
      841 ttggccaggc cggcgaccgc gagcaggttg gcggacgagc cggaattcac catgacggcg
      901 tggccgtgtc cgaaccgctt cgcgaactcc tgctcgaact gccggacctt cggccccatc
      961 gtgacttcgg tgtggagcag gcagtcgagg accgcggcga tctcttcatc gtcatacgtc
     1021 gcctcgtgga gcttgaccac gggccgggcg gggtcgaacg gcttcggcgc cgtctgtttc
     1081 caggcggccc ggagggcgtc gcgaagaggg gagtcgtcca cgtcagttca gttccggctc
     1141 aggtaccatt cgtaggtccg ccgcagcccg tcctggaggg acgtgcggga ggcccatccc
     1201 aggctgtgga tgcgggagga gtcgagcacc ttgatgggcg cgccgtcggg cttgctcgca
     1261 tcgaactgga tcgagcctcg gtatcccaga aggccggcga tcgactccgc cagctccttg
     1321 atcgacgtgc cccggcccgt gccgatgttg atcggcgcgc cggggagcgg cccgggaagg
     1381 tccatcagca ggacgcacgc ttcggccagg tcgtcgacgt aaaggaactc gcgaaccggt
     1441 cggccggtcc cccagaccgt gacggaggat tggcggcgct gctcgtggaa gcggcgaatc
     1501 agggcggaca ggacgtgcga cgtcgccgga tcgaagttgt cgccgggtcc gtacagcgtc
     1561 gccgggatga cggtcaggaa ctgcgtgccg tactggcggt tgtaggcctc gcacatcatg
     1621 tagccggcga gcttggaggt cgagtaggcc tcgctggtct gttccatcgg gcccgtcagg
     1681 atcgcctcct ccttcatggg ctgggggcac tcgcggggat agaggcagga gccggcgaag
     1741 aagatgagcc gcttcacgcc gctccgatgg gccgcgtgga gcaccgacgt ctggatgagc
     1801 aggttgtcgc ggatgaagtc ggcgggcgac gcctggttgg cctggatgcc gccgacgcga
     1861 ccggccgcaa ggaagacgta ccccggcttc tcgcggagga agaactcctc gacggcctcc
     1921 cggcgggtga ggtccagctc ctcccgggtg cggaggacga gccgggagtg gccggcggcg
     1981 cgcagccgtt tgaccagcgc cgaccccacc attccccggt gaccggcgac gtagacggag
     2041 gcgtgaggat ccatgcgaag tacctgattg taccacccca gaatcgagaa tgaagaatcg
     2101 agaatcgaaa cgaccatgct gctacagcag gccgtcctgc gcgacgtagc tggtccgatc
     2161 gacgcccgcc ggc
//
LOCUS       DASRIQ010000208         6543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1899945, whole genome shotgun sequence.
ACCESSION   DASRIQ010000208 DASRIQ010000000
VERSION     DASRIQ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899945"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFD06_05125"
     CDS             complement(<1..566)
                     /locus_tag="VFD06_05125"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HZN02949.1"
                     /translation="MKRFRDRSITQKLTLIVLFTTGAALVTTCCLFLGYEWATARAGM
                     EREITTLAEFLGANTNAALAFHDRKAASETLSTLRAQRPITAAALYDNDGRLVASYRR
                     LGRMSPAPATPPSLGDPVWGGGRLTLTRPVFLEGDRLGTLWLEADLEEMEGRMQRFTA
                     VAASVLLLAAGLALLLSARLQRFITGPIG"
     gene            complement(563..1129)
                     /locus_tag="VFD06_05130"
     CDS             complement(563..1129)
                     /locus_tag="VFD06_05130"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HZN02950.1"
                     /translation="MCAFLLLNLLAGPVAAQPGPEATLPPSVEYQIKADFVYTVAKFV
                     DWPEKAFAGPGAPLTFGIVGSDVVADAIAQALRGKKVHERPIRTVRITDLRRALECQI
                     VYVSESDPSRVKALLEEVEAASILTVGEGSDFADRGGILGISMRDTLVQFEVNMRSAE
                     RAGIAISSKILRLGEVVGAARPAAEGRK"
     gene            complement(1363..2229)
                     /locus_tag="VFD06_05135"
     CDS             complement(1363..2229)
                     /locus_tag="VFD06_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZN02951.1"
                     /translation="MSQLGAGPRLRLGTSSWTAPSWEGPFYPKGLPPARWLEHYATQF
                     DSVEIDATFYRIPEERTVDGWRDRTPPGFLFSAKVPQVVTHEKMLENCLEEMTAFLKV
                     MQRLGDRLGPLLLQFRYFRKAELPDPKPFLERLERFLPSLPPTMRFAVEVRNRAFLGD
                     RLFDLLRRQEIALAFIDHPWFPGVGEMMKRRAARTAPFSYIRWLGDRVAIEKRTTTWD
                     ALIVDRRAEMRRWAEAVRTLQREERTVHGYFNNHYAGYGVGSIRLFRDILDESAGNSE
                     PPARSPGPGVEV"
     gene            complement(2249..2764)
                     /locus_tag="VFD06_05140"
     CDS             complement(2249..2764)
                     /locus_tag="VFD06_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZN02952.1"
                     /translation="MSKGKIAILVENDYQDMEVWVPLYRLREEGYQTVVLGTGAPSYK
                     SKHGYPVAAEMKASEARAADFAGVVVPGGWAPDRLRMDAGVLRFVREIDAQGGIVAAI
                     CHAGWVLASAGIVKGRKVTAYQAIRDDLVHAGATFVDQEVVRDRNLITSRKPDDLPAF
                     CRELVRALEAS"
     gene            2834..3322
                     /gene="bfr"
                     /locus_tag="VFD06_05145"
     CDS             2834..3322
                     /gene="bfr"
                     /locus_tag="VFD06_05145"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HZN02953.1"
                     /translation="MKGDPKVLTLLNQVLRKELTGINQYFLHARMCANWGYHVLAKRL
                     RTESIDEMKHADLVMERLLFLDGTPNMAEIDRLFIGKNVRDQLKSDLGLEMAAIEVLR
                     PGIDLCVKVSDHATRELLEHILEEEEHHVDWLEAQLHQIEEMGYENYLTTLVGGEEVK
                     AG"
     gene            complement(3319..3786)
                     /locus_tag="VFD06_05150"
     CDS             complement(3319..3786)
                     /locus_tag="VFD06_05150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN02954.1"
                     /translation="MSDRPGPADFPFAAPIEVRFRDMDGMGHVNNAVFLTYFEVARAH
                     YWKAVGEDEWSRLRTYVLARAEVDFRSPVTLADELVCHVRVAAFGTRSFAMEYLLVDR
                     KSGRTVAEGRTVQAMYDYEKNASRPLDEAMKAAVRRFEKRAIPEQSRAGGAGG"
     gene            complement(3783..4574)
                     /locus_tag="VFD06_05155"
     CDS             complement(3783..4574)
                     /locus_tag="VFD06_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN02955.1"
                     /translation="MLLEGRVAVITGGGRGIGRAIAERFYREGAKVAIASRNAKTLQT
                     VAMEMNKGDHRIVPFRCDVSNRDEVEVLFGNVIEVWDRVDILVNNAGLSGITPAMPAP
                     EGDETEARFRQILDVNVTGTWFCVREAVPHMPEDGRGRILNISSVLGKFGVPGYGGYC
                     ASKHGVIGLTKALALELAPKKITVNALCPGWVETDMAREGVEAGARSERITPEEFRRR
                     AEEAVPLGRFLRPEEVADLATFVASDAAAGMTGQAINLDGGSAMW"
     gene            complement(4590..5147)
                     /locus_tag="VFD06_05160"
     CDS             complement(4590..5147)
                     /locus_tag="VFD06_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN02956.1"
                     /translation="MSPVDLFHDDGSVPYRFCPRCGGGLESRVVFSHDPPRLVCGACG
                     FVFYLDPKVAAGAICRMDGGVVLLRRGIEPGYGLWVYPGGYVDRGESLEEAAVREARE
                     EVGLEIRLTGLVNAYSYPGRPVIVVVFAADVIGGALTRGEEALDAQVFPPGDIPWGDL
                     AFRSTTDALKEFFGGAARRFQPEPR"
     gene            complement(5144..5896)
                     /locus_tag="VFD06_05165"
     CDS             complement(5144..5896)
                     /locus_tag="VFD06_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN02957.1"
                     /translation="MHGEHGGAVVSRFDSEARDFAKSPFHRDPARLKRLLDFAAVTPE
                     EEALDLACGPGIVAQALRERGASVTAIDLTPGMLREARTSGARLLRADALRLPFREAA
                     FDLVLCRNSFHHFPDPAGAFAEAARVLRPGGRLAYEDMAAAEDLRERDLQETIERLRD
                     RAHARTLPPGEVRALLAATGLRESASLLVPMTVDFDEWIDRPRPSAAARARARFLMES
                     RLGAPAGTLRSFVEEGRLRFERASLLAIAVKG"
     gene            5947..>6543
                     /locus_tag="VFD06_05170"
     CDS             5947..>6543
                     /locus_tag="VFD06_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02958.1"
                     /translation="MTRAAFATAVLTGLLALPPAAGAADTAGPAKPGSAASLDVIQLH
                     VGIPGTVWLGASMPEILARFPDVKQLPFANQPYVIRLQIPSEGISALAMGDTPASMTI
                     ESIGFNFAEPYEGVQAGPWRTVEGIGAGSNVNELLEIYGKPAESGPEERRGMLAPAPG
                     GAGGGSGRVRHLYRSADGSVATYFVVEGSRVLRMAMSRP"
BASE COUNT         1010 a         2392 c         2225 g          916 t
ORIGIN      
        1 ccgatcggcc cggtgatgaa gcgctgcagc cgggcgctga ggaggagcgc cagcccggcg
       61 gcgagcagga gcaccgaggc ggcgaccgcg gtgaagcgct gcatccgccc ttccatctcc
      121 tcgagatcgg cttcgagcca cagggtgccg aggcggtccc cttcgaggaa gaccggccgg
      181 gtcagggtga gcctcccgcc gccccagacc gggtcgccga gcgacggcgg ggtggccggc
      241 gccgggctca tccgtcccag gcgacgatac gaggcgacca gccgcccgtc gttgtcgtac
      301 agggcggcgg ccgtgatcgg ccgctgggcg cgcagcgtgg agagggtctc ggaggccgcc
      361 ttgcgatcgt ggaacgccag agcggcgttg gtgttggcgc cgaggaactc cgcgagcgtc
      421 gtgatctccc gctccatccc ggcccgcgcc gtcgcccact cgtatccgag gaacaggcag
      481 caggtcgtga cgagcgccgc cccggtcgtg aacagcacga tcagggtcag cttctgggtg
      541 atcgaccggt cgcggaagcg cttcacttgc gcccctccgc cgccggccgc gccgcgccga
      601 cgacctcccc cagacggagg atcttgctgc tgatggcgat gccggcgcgc tcggcggagc
      661 gcatgttcac ctcgaactgc acgagggtgt cgcgcatcga gatccccagg atgccgccgc
      721 gatccgcgaa atcgctcccc tcgccgaccg tcaggatgct cgccgcctcg acctcctcga
      781 ggagagcttt cacccgggac ggatcggact cgctcacgta gacgatctgg cattcgaggg
      841 cgcggcgcag gtcggtgatc cggacggtgc ggatcggacg ctcgtgcacc ttcttgccgc
      901 gcagcgcctg ggcgatggcg tcggcgacga cgtcgctccc gacgatcccg aaggtgaggg
      961 gggcgccggg gcccgcgaag gccttctcgg gccagtcgac gaacttggcg acggtgtaga
     1021 cgaagtccgc tttgatctgg tactcgaccg aggggggaag ggtggcttca ggaccgggct
     1081 gcgccgcgac cggcccggcg aggaggttca ggaggagaaa tgcgcacacc gccgcgactt
     1141 cgcggcggcg cacccgtgcg atgggtggac gctccccctc gaccgacaag ccggacccct
     1201 ccgcgatccg ggacgccagt caccctgccc ggacggcggc agcaatataa ggtccggccg
     1261 cggggcgatc caccgctgga caccgattct gccaaaggat tgacacaggg gagccggcga
     1321 ccgggccgca ccccggcagg accgggcggc gccccggcgc ggtcagactt cgacccccgg
     1381 gccgggactc cgggccgggg gttcgctgtt tccggcggac tcgtccagga tgtcccggaa
     1441 cagacggatc gaccccaccc cgtagccggc gtagtggttg ttgaagtagc cgtgcaccgt
     1501 ccgctcctcc ctctggagcg tccgcaccgc ctccgcccag cgccgcatct ccgcgcgccg
     1561 atcgacgatg agggcgtccc aggtggtggt gcgcttctcg atcgccaccc gatccccgag
     1621 ccagcggatg tacgagaacg gcgcggtgcg cgccgcccgg cgcttcatca tctcgccgac
     1681 gcccggaaac cacgggtgat cgatgaacgc cagcgcgatc tcctgccggc gcagcagatc
     1741 gaagagccga tctccgagga acgcccggtt gcgcacctcg acggcgaagc gcatcgtcgg
     1801 cggcagcgac ggcaggaagc gctccaggcg ctcgaggaac ggcttcgggt ccggcagctc
     1861 cgccttgcgg aaatagcgga actgcagcag gagcggcccg agccggtcgc cgagccgctg
     1921 catcaccttg aggaaggcgg tcatctcctc gaggcagttc tcgagcatct tctcgtgcgt
     1981 gacgacctgg ggaaccttgg ccgagaacag aaagccgggc ggagtgcggt cgcgccaccc
     2041 gtccacggtc cgctcctccg ggatgcggta gaaggtggcg tcgatctcga cgctgtcgaa
     2101 ctgcgtcgcg tagtgctcca gccaccgggc cggcggcaac cccttcgggt agaacggccc
     2161 ctcccaggag ggggcggtcc aggaggaggt ccccaggcgc agccgaggtc cggcgccgag
     2221 ctggctcacg acccgatgcc ggttgcgctc acgacgcctc gagggcgcgg accagttcgc
     2281 ggcagaacgc cggcaggtcg tccggcttgc gcgaggtgat caggttgcga tcccgcacca
     2341 cctcctgatc gacgaaggtc gccccggcgt gcaccaggtc gtccctgatc gcctggtacg
     2401 cggtgacctt acgtcccttg acgatgccgg cggaagccag aacccagccg gcgtgacaga
     2461 tggccgccac gatccctccc tgcgcatcga tctcccgcac gaagcgcagg acgccggcgt
     2521 ccatgcgcag tcgatccggg gcccagcctc ccggaaccac gaccccggcg aagtcggcgg
     2581 cgcgcgcctc ggaggccttc atctcggcgg cgactggata gccgtgcttc gacttgtagc
     2641 tcggcgcgcc ggtgccgagg acgaccgtct gatacccctc ctcgcgcagc cggtacagcg
     2701 gcacccacac ctccatgtcc tggtagtcgt tctccaccag gatcgcgatc ttccccttgc
     2761 tcatgtcgct cctcctctct tagactgcgg ccttcctgac agccgctctg acgcacgcac
     2821 cgacggaggc cgaatgaaag gcgatcccaa ggtcctgacg ctcctcaacc aggtcctccg
     2881 caaggagctg accgggatca accagtactt cctgcacgcc cgcatgtgcg ccaactgggg
     2941 ctaccacgtc ctcgcgaagc gcctgcggac ggaatcgatc gacgagatga agcacgccga
     3001 cctcgtcatg gaacggctgc tcttcctcga cggcactccc aacatggcgg agatcgaccg
     3061 gctgttcatc ggcaagaacg tccgtgacca gctgaagagc gacctcggcc tggagatggc
     3121 ggcgatcgag gtcctgcgcc ccggcatcga cctgtgcgtc aaggtgtccg atcatgccac
     3181 ccgcgagctc ctggagcaca tcctcgagga ggaggaacac cacgtcgact ggctcgaggc
     3241 ccagctgcac cagatcgagg agatgggcta cgagaactac ctgaccaccc tcgtcggggg
     3301 ggaagaggtg aaggccggct agcccccggc tccccccgca cggctctgct ccgggatcgc
     3361 ccgcttctcg aagcgccgca ccgcggcctt catcgcctcg tcgagcgggc ggctggcgtt
     3421 tttctcgtag tcgtacatcg cctgcaccgt ccgcccctcg gcgacggtgc gtccgctctt
     3481 ccggtcgacg agcaggtact ccatggcgaa cgagcgggtc ccgaatgcgg cgacccgcac
     3541 atggcagacg agttcgtcgg cgagggtcac cgggctccgg aaatcaacct ccgcccgcgc
     3601 caggacatag gtgcgcaggc gcgaccactc gtcctcgccg accgccttcc agtagtgtgc
     3661 ccgcgcgacc tcgaagtagg tcaggaagac ggcgttgttg acgtgcccca tgccatccat
     3721 gtcgcggaag cggacctcga tcggcgccgc gaacgggaag tccgcgggtc cgggacgatc
     3781 gctcaccaca tcgccgaccc gccgtccagg ttgatcgcct ggccggtcat ccccgccgcc
     3841 gcgtcggagg cgacgaatgt cgcaagatcc gcgacctcct ccggccgcag gaagcggccg
     3901 agcggcacgg cctcctccgc gcggcgccgg aactcctccg gggtgatccg ctcgctccgc
     3961 gcccccgcct cgaccccttc cctcgccatg tcggtctcga cccagcctgg gcacagggcg
     4021 ttgacagtga tcttcttagg cgccagctcg agcgccagcg ccttggtgag gccgatgacg
     4081 ccgtgcttgc tggcgcagta gcctccgtag ccggggaccc cgaacttgcc gaggaccgac
     4141 gagatgttga ggatgcggcc gcgcccgtcc tccggcatgt gcggcaccgc ctcgcgcacg
     4201 cagaaccagg tcccggtgac gttgacgtcg aggatctgcc ggaagcgcgc ctccgtctcg
     4261 tccccttccg gcgccggcat cgccggcgtg atgccggaca ggccggcgtt gttgacgagg
     4321 atgtcgaccc ggtcccacac ctcgatgacg ttgccgaaga gcacctcgac ctcgtcgcgg
     4381 ttggagacgt cgcagcggaa cggcacgatg cgatggtccc ccttgttcat ctccatggcg
     4441 accgtctgca gcgtcttggc attgcgcgac gcgatcgcca ccttcgcccc ctcgcgatag
     4501 aaacgctcgg cgatggcgcg gccgatgccg cggccgccgc cggtgatcac cgccacccta
     4561 ccctccagca gcattcctcc ccctctctct cagcgtggtt cgggctggaa gcgccgcgcg
     4621 gcgcccccga agaactcttt caacgcgtcg gtcgtgctgc ggaacgccag gtccccccat
     4681 ggaatgtcgc ccggagggaa gacctgcgcg tcgagcgcct cctccccccg cgtcagcgcg
     4741 ccgccgatga cgtcggcggc gaacaccacg acgatcaccg ggcgccccgg gtacgagtag
     4801 gcgttgacca gaccggtcag gcggatctcg aggccgacct cctcgcgcgc ctcccgcacc
     4861 gccgcctcct cgagcgactc gccccggtcg acgtagccgc cgggatagac ccacaggccg
     4921 taccccggct cgatgccgcg ccgcaggagc acgacgccgc cgtccatgcg gcagatggcg
     4981 ccggccgcga ccttcgggtc gagatagaag acgaagccgc aggcgccgca gacgagccgc
     5041 ggcgggtcgt gcgagaagac caccctcgac tcgagcccgc cgccgcagcg cgggcagaag
     5101 cggtacggaa cgctgccgtc gtcatggaac agatcaaccg ggctcatccc ttgaccgcga
     5161 tcgccagcag gctcgcccgc tcgaaacgca ggcgcccctc ctcgacgaac gaccgcagcg
     5221 tccccgccgg ggccccgagg cgcgactcca tcaggaaccg ggcgcgggcg cgggccgcgg
     5281 cggaggggcg cgggcgatcg atccactcgt cgaaatcgac cgtcatcggg acgaggagcg
     5341 acgcgctctc ccgcaggccg gtcgccgcga ggagcgcgcg cacctccccg ggcggcaggg
     5401 tgcgggcgtg cgcccgatcg cggagccgtt cgatcgtctc ctgcaggtcg cgctcgcgca
     5461 gatcctccgc cgccgccatg tcctcgtacg ccagccggcc cccggggcgc agcacgcgcg
     5521 ccgcctcggc gaaggccccc gccgggtcgg ggaagtggtg gaaggagttg cggcacagga
     5581 cgagatcgaa cgccgcctcc cggaacggca gccgcagcgc gtcggcgcgc agcaggcggg
     5641 cgccgctcgt ccgcgcctca cgcagcatcc ccggcgtcag atcgatcgcc gtgaccgagg
     5701 cgccgcgctc gcggagcgcc tgcgccacga tgccgggccc gcaggcgagg tcgagagcct
     5761 cctcctccgg cgtcaccgcc gcgaagtcga ggagccgctt cagccgcgcg ggatcacggt
     5821 ggaacggact cttcgcgaag tcgcgggcct ccgaatcgaa gcggctcacg accgcgccgc
     5881 cgtgctctcc gtgcaacggg atctcctcgg gaagggccga ttgtaccgca tgctatagtg
     5941 ccgacgatga cgcgcgcggc gttcgcaacg gcggtgctca cgggcctcct ggcgcttcct
     6001 cccgccgccg gagcggcgga cacggccggc cccgcgaagc ccggaagcgc ggcctcgctc
     6061 gacgtcatcc agctgcacgt cggcatcccc ggcaccgtgt ggctcggcgc gtcgatgccg
     6121 gagatcctgg cccgcttccc cgacgtgaaa cagttgccgt tcgcgaacca gccttacgtg
     6181 atccggctgc agatcccctc ggaggggatc tcggcgctgg cgatggggga cactcccgcg
     6241 tccatgacga tcgaatcgat cggcttcaat ttcgcggagc cgtacgaagg ggtgcaggcc
     6301 gggccctggc gcaccgtcga ggggatcggc gccggctcga acgtcaacga gctgctcgag
     6361 atctacggca agccggccga atccggcccc gaggagcgcc gcggcatgct ggcgccggct
     6421 ccgggcggcg ccggcggcgg atcgggccgg gtccggcacc tgtaccgcag cgccgacgga
     6481 tcggtcgcga cctatttcgt ggtcgaggga agccgcgtgc tccgcatggc gatgagccgg
     6541 ccc
//
LOCUS       DASRIQ010000209         9435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2955975, whole genome shotgun sequence.
ACCESSION   DASRIQ010000209 DASRIQ010000000
VERSION     DASRIQ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2955975"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..610
                     /locus_tag="VFD06_05175"
     CDS             278..610
                     /locus_tag="VFD06_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN02959.1"
                     /translation="MAERTELLRGTLDVLILKALALGENHGLGIARRVEQLTRGAFEV
                     KAGSLFPALHRLEERGWLDATWGESETRRRARYYRLTAAGRRRLREETGQWRRIAAAI
                     SQALAATS"
     gene            621..3320
                     /locus_tag="VFD06_05180"
     CDS             621..3320
                     /locus_tag="VFD06_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN02960.1"
                     /translation="MPLLRRLRSLARNVLRRDQVEHDLDREIESFLQDLTDERIAAGM
                     PPEQARREARLALGGAEQVREEVRAVRAGSFLDALAQDLRFGARMLRRAPAFTAVAVV
                     TLALGIGAVTSIFSVVHAILLRPLPYPEPDRLVLLRQAYPQVGLTFWRLSQANFLAYR
                     DRATSFETAGAYTYAGYNVSGGGEPARVQAAAVSANLFATLGAKAALGRTFLPGEDHP
                     GRRLLVLLSDGEWRRRFGGDPGVVGRTLLLDNVPAEIVGVMPKDFDFPNRAVQIWTPL
                     DLDPSRTSPYLLLLVARLRPGVTPQAAQEETTGILTTMSAAQPAFAGGPTPPGPGADL
                     HTVVRPLGEALTSRARTPLLLLFAATGLLLLIACANVANLVLARSTARAREIAVRFAL
                     GATAARVGRQLLTELLLLGALGGAAGLLVALPILAAARRLPTGFLPRLEEVRLDPSVL
                     AAALVSGLAAAILAGLLPALRLARGGAASDRPLQARTTAAPGGRRASGALVGLQFALS
                     LLLLVGSGLLLRSLHRLMAVDPGFDPSRLMTLSLDLPPRQAGVYANPLGAVAEAEQEG
                     TARFYDRVVEAVRAQPGIGDAALASSLPFGADLDSDMTVCERREGKAGEEGSLTLIVR
                     TSPGFFRTLGLPLLRGRDFTETDRVGGAPVAIVDETLAAAAWPGEDAVGRRIRYRWDD
                     RPERWKTIVGVVAAVRDDSLDHGAEPHMYIPFGQEPRRRMGLVARSATDDAGAVAGGI
                     RAALRSVDPTVPAFDVRSMEETMGLSLFERRFTNLLLGAFAATALLLAAAGIYGVMSL
                     EVASRFKEIAVRMALGARPGNVFRLVLRHGATLAAAGMAAGLLGSLAMTRLLSGLLFE
                     VRPSDPPTLCAVVLILGIVALLACAAPARRATRVDPIAALRQD"
     gene            complement(3352..3570)
                     /locus_tag="VFD06_05185"
     CDS             complement(3352..3570)
                     /locus_tag="VFD06_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02961.1"
                     /translation="MLLAVVPAGGFDALSVRAASDKAPAGRFALLLEEMDGGPDLALA
                     FARHLEGSADAEPGAGKERLKASRAARD"
     gene            complement(3998..4324)
                     /locus_tag="VFD06_05190"
     CDS             complement(3998..4324)
                     /locus_tag="VFD06_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02962.1"
                     /translation="MTVGMLVLVGLTGQFWAVRPADTPRRDSDAAGGLRLGLAAGAAV
                     APAQSAAMRRLESESGARLRVSHNGLTRVPRTLGSDRPLSPPSSADRALLATGGSSRG
                     LLMQLP"
     gene            complement(5505..8009)
                     /locus_tag="VFD06_05195"
     CDS             complement(5505..8009)
                     /locus_tag="VFD06_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN02963.1"
                     /translation="MGGIRWNDVGGLRWNDVGGIRWNDVGGTLFTDASGIRWNDVGGI
                     RWNDVGGLQFDEPPDAPSIDPALLNLLSTLPDTSSIDVIVTYRLMPSTADLATLTALG
                     ILGGTKFRRLPMVVVDATPEQIRAIAVLPAVRSLYADRTLSFFDADSRDLIGMPDVET
                     DAALRSPGGAPLTGAGVTIAVLDTGVDGTHPDLPYGSKVIGNVRLNGTGGIGLGFRYP
                     AAVEGLINTDLVLGHGTAVASVAAGSGAASAGTYKGVAPSASILSLSAGDVFILKVLE
                     GFDYLLDNRARYGVRIVNCSWGTSGFFDPSDPVNVATRLLFDEGVTVIFAAGNHGPAP
                     DTLNPYAVAPWVIGVGSVGRDGRLSGFSSRGIFEEILSHPTLVAPGEGIVAANPLAVG
                     MVNGIVGVADPAGGAVVAPAYALWYSAVSGTSFAAPHVAGVAALMLEASPALTPAALK
                     RILQATATPILARERSEAGAGRLEAWAALTQATDAARPFGTFVPGWLDERRYAYQHQA
                     ALSSGLVPWSGALALPVTLDAAAAAWGMSIAWGDELAGLDDLDLILEDEHGAEVGRSE
                     SINATVLFAHTEGVQLAGAMPRTMTARIVHKLGVGPADRTVSIRQETAVAVLTGFTDL
                     GQLGSTDRDLLARAVSRHVLDGRGDRFEPSASLRRGELARSLALVADRPQRVPALSSF
                     TDVPPSSPDFPFVESAAGQRAAVVLIDPEDPLRFKPADAVDRLDFAVAMVRAAGRAAE
                     ADARAGEALGLKDDTQIPPALRGYAAVALERGFIETVPSSKGARFAPAGSVPRLAAAR
                     FLLRLLGDRNGGLSLVPMRDVLSVLPSLIRVEPRPQ"
     gene            complement(8239..>9435)
                     /locus_tag="VFD06_05200"
     CDS             complement(8239..>9435)
                     /locus_tag="VFD06_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZN02964.1"
                     /translation="VVERARAAGRREGLASALLAAGETEVRAGRDDRAVLALEEAAAI
                     FSGLDRAYPSCLALAWLAFARHRQGAADPAGRAAETALLLSARFDYEAALLRVADLDP
                     GFRRWLGARAGAPAALRADGMEAPRRSAPAPGGADLAVRLFGPVEVFRDRDQAIPPRA
                     WKIRRALTIFCYLAVARDRRATKERIVDALWGDARPAVIEKNFHPTISFLRRALNDGH
                     NVPRHFILFERGAYRLNPAYRYDIDAEKFEAGIREARRLQAAGDATAALAAYDAALAL
                     YRGPFLEEEYDEWIEGPRAHYEVLMAAALREAGRLHVERGDAGAGLGYLGRAVAQDAI
                     DEAASADLMLALGRLRRRAEIEKEHERLRKALLDGLSAVPSTSTRQAFERALRLAAEP
                     PPAGRR"
BASE COUNT         1295 a         3470 c         3450 g         1220 t
ORIGIN      
        1 cggatccggt gccgcccaat gacggcgggt tcttcgccat gggaggctcg gattcgcgcg
       61 gccgcgacag cgtcggcttc aacagcctgc acgccggcgt cgccttcaag ttcggcaaag
      121 gaacgtagcg ggccggcgcg cgagccgcag ggatgcgccc agggggcggc tccggccgcc
      181 cccttttttt cgccacgccc cgtcgccggg gcggcgtgga ttgacacgca tcctcccgcc
      241 gtcgtatcgt cctatagact ttctatagga gagacgcatg gcggagcgga cggaactcct
      301 gcgcggcacg ctcgacgtgc tgatcctcaa agcgctcgcc ctcggcgaga accacggcct
      361 cggcatcgcg cggcgcgtcg agcagctgac gcgcggcgcc ttcgaggtca aggccggctc
      421 cctcttcccg gccctgcacc gcctcgagga gagggggtgg ctcgacgcga cctgggggga
      481 gtcggagacc cgccgccgcg cccgctacta ccgcctcacc gccgccggcc ggcgccgtct
      541 ccgcgaggag acggggcagt ggcgtcgcat cgccgccgcg atctcgcagg cgctggcggc
      601 gacgtcctga ggaggccgcc atgccgctgc tccgccgcct ccgaagcctc gcccgcaacg
      661 ttctccgccg cgatcaggtc gagcacgacc tggaccgcga aatcgaaagc ttcctgcagg
      721 acctgaccga cgagcggatc gccgccggga tgccgccgga gcaggcgcgc cgcgaagcgc
      781 gcctggccct cggcggggcc gagcaggtga gggaggaggt gcgggccgtg cgcgccggct
      841 ccttcctcga cgcgctggcg caggacctgc gcttcggcgc ccgcatgctg cggcgcgccc
      901 ccgccttcac ggccgtcgcc gtcgtcacgc tggcgctcgg catcggcgcc gtcacgtcga
      961 tcttcagcgt cgtccacgcc atcctcctgc gtccgctccc ctacccggag ccggaccggc
     1021 tcgtcctgct gcgccaggcg tatccacagg tcgggctgac cttctggcgt ctgtcgcagg
     1081 cgaacttcct ggcctaccgg gatcgcgcca cgagcttcga gacggccggc gcgtacacgt
     1141 acgccggcta caacgtctcg ggcggcggcg agccggcgcg cgtccaggcg gcggcggtga
     1201 gcgcgaatct gttcgccacg ctcggcgcca aagcggcgct cggacgcacc ttcctcccgg
     1261 gggaggacca ccccggtcgc cgtctcctcg tgctcctgag cgacggggaa tggcggcggc
     1321 gtttcggcgg cgatcccggc gtggtcgggc gcacgctcct gctcgacaac gtcccggcgg
     1381 agatcgtcgg ggtcatgccg aaagacttcg acttccccaa ccgcgccgtg cagatctgga
     1441 cgccgctcga cctggatcct tcgcggacct ctccctactt gctgctcctg gtcgcgcggc
     1501 tgcgccccgg cgtgaccccg caggcggcgc aggaggagac gaccgggatc ctgacaacga
     1561 tgagcgccgc ccagccggcc ttcgcgggcg ggccgactcc tccggggccc ggcgccgacc
     1621 tgcacacggt cgtccggccg ctcggcgaag cgctcacctc acgggcgcgc actccgcttc
     1681 tcctcctgtt cgcggcgacg gggctcctgc tcctgatcgc ctgcgccaac gtcgccaacc
     1741 tggtgctggc ccgttcgacg gcgcgcgccc gggagatcgc cgtccgcttc gcgctcggcg
     1801 cgacggccgc gcgcgtcggc cggcagctcc tgacggagct tctgcttctc ggcgctctgg
     1861 gcggggccgc cgggctcctc gtcgcgctgc cgatcctcgc ggcggcgcgg cgcctgccca
     1921 ccgggttcct gccgcgcctc gaagaggtgc gtctcgatcc gtcggtgctc gcggcggcgc
     1981 tcgtctccgg cctcgccgca gcgatcctcg ccggactgct gccggccctg cgcctggcgc
     2041 gcggcggcgc cgcgagcgat cggccgctcc aggcgcgcac taccgcagca ccggggggcc
     2101 gccgcgccag cggcgcgctc gtcggcctgc agttcgcgct gtcgctcctc ctgctcgtgg
     2161 gatccgggct cctcctccgg agcctgcacc gtctcatggc ggtcgatccg ggcttcgatc
     2221 cgtcgcggct gatgaccttg agcctcgatc tccccccgcg ccaggccggc gtgtacgcga
     2281 acccgctggg agctgtcgcc gaagcggagc aggaggggac ggcgcgcttc tacgacaggg
     2341 tcgtcgaggc ggtccgcgct cagccgggga tcggggacgc cgccctcgcc tcgagccttc
     2401 cgttcggcgc cgatctcgac tcggacatga cggtgtgcga aaggcgcgaa ggcaaggcgg
     2461 gggaggaggg ctcgctgacc ctcatcgtgc ggaccagccc gggctttttc cggacgctgg
     2521 ggctgccgct cctgcgcggt cgggacttca ccgagaccga ccgcgtcgga ggggcgccgg
     2581 tcgccatcgt ggacgagacc ctggcggcgg ccgcctggcc cggcgaggac gcggtcgggc
     2641 gacgcatccg ctaccgctgg gacgaccgcc ccgagcggtg gaagacgatc gtcggcgtcg
     2701 tcgccgcggt ccgcgacgac agcctcgacc atggcgccga gccgcacatg tacatcccct
     2761 tcgggcagga gccgaggcgg cgcatgggcc tcgtggcccg ctccgcgacc gacgacgcgg
     2821 gagcggtggc cggcgggatc cgggcggcgc ttcgaagcgt cgatccgacc gtcccggcct
     2881 tcgacgtccg cagcatggag gagaccatgg ggctgagcct gttcgagcgg cgcttcacca
     2941 acctcctgct cggcgccttc gcggcgacgg cgctcctgct cgccgcggcg gggatctacg
     3001 gcgtgatgtc gctcgaagtc gccagccgct tcaaggagat cgccgtgcgc atggcgctcg
     3061 gggcccgccc cgggaacgtg ttccggctcg tcctgcggca cggcgcgacg ctggcggcgg
     3121 ccggcatggc ggcggggctt ctcggctcgc tggcgatgac gcggctgctc tccggcctgc
     3181 tgttcgaagt ccggccgtcc gatccgccga ccctctgcgc ggtggtcctg atcctcggaa
     3241 tcgtggcgct gctcgcgtgc gccgcgccgg cccggcgcgc cacgcgggtc gacccgatcg
     3301 ccgccctgcg ccaggattga ccggttccgc ggagcgctga cggcaggcgg ctcagtcgcg
     3361 cgcggcgcgc gacgccttca ggcgttcctt ccccgcgccg ggctcggcgt cggccgagcc
     3421 ctcgaggtgc cgcgcgaaag caagcgcgag gtcagggcca ccgtccatct cctcgagaag
     3481 cagcgcgaag cgccccgccg gagccttgtc ggaggcggcg cgcacgctca gcgcatcgaa
     3541 cccaccggcc gggacgacgg cgaggagcac ctcctcgccg ctcgagcgcc gatccgattc
     3601 gaccgccggc ccaccggcgc cggaagcgag cgccaggacg acgtcgacgc ccgtctggac
     3661 gaagggggcg cgcgggcgcg tccgcccgga acgccggttc gagggacact ccccccaggc
     3721 ccacgagggc cagcacgacg gcgcggcggg cgcgccggtc tcgctgctgt cgagttcctg
     3781 cgtgcatcgg cggagcccct tcggcgatgg gggagcccgg actccgctcg gggcgggatc
     3841 gggccgctcg acgcatcgta ccgaaccggc gctccggccg ggggcgaaaa aaaaggcagg
     3901 cgcgatccga gggagatcgc gcctgcctgc tccgggtcag ggggagggca cgccagcggg
     3961 gccgccctcc ccctccccca ccccccccgg ggccggacta cgggagctgc atcagcaggc
     4021 ccctggaaga cccgccggtc gccagcagcg cccgatctgc ggaggaaggc ggcgagagcg
     4081 gcctgtcgga accgagcgtg cgcgggaccc gcgtcaggcc gttgtggctg acgcgcagtc
     4141 tggcgccgct ctccgactcg agacggcgca tcgccgctga ttgcgccggc gcgacagccg
     4201 ctcccgccgc cagtccgaga cgcagtccgc ctgctgcgtc ggaatcgcgc cgcggcgtgt
     4261 ccgccggcct gacggcccag aactgtccgg tgagaccgac caggacgagc atgcccactg
     4321 tcatgaggat gattcgcctg ggagagacgt tcatgttccg gacctccatt gggtgtttcg
     4381 ggcttggagg ctgccccgct ggttcgcgcg caaggtgcgc cggggggctt gaacgatcct
     4441 tgaaccggac cgtctctgaa gggggtgggc ggagcctaag acgctgcggc ggtggtggtt
     4501 acggtttttc tgcgaaaaac gcccttgccg gcttgccccg ggcgggcgcc tgcggcgtca
     4561 ctgcggccgc ggctcgacta gagaggctgc cgttccgatc gccgaggagc ccgaggagga
     4621 agcgggcggc cgccaggcgc ggcaccgatc ccgccggaac gaagcgtgcg cccggtgggg
     4681 acgggatggt ggcgatgaac ccacgctcga gcgcgacggc gacgtagcct ccccaggccg
     4741 agaagggcca gcacgacggc gtgacaggcg cgctgagacg tgcgggctgg agctcctgcg
     4801 cgcatcgacg gtgcctcttc gcctttcgcg atgaacccgg attcagctcg gcgcggggcg
     4861 ggcgatgcgc agcatcgtac ctaaaaaaaa ggcaggcgcg atggagagag agagatcgcg
     4921 cctgcctcct ccgggtgggg ggagggcctg ccagcggggc tgccctcccc tccccggagc
     4981 cgaactacag gagctgcatc aggagaggat ccgcggacga aggcggcgag agcggcttgt
     5041 cggaacggag cgtgcgcggt acccgcgaca ggccattgtg gctgacgcgc agtcgaccgc
     5101 cgctctccga ctcgagacgg cgcatcgccg ccgttgcgcc ggcgcgacga ccgctcccgc
     5161 cgccagtccg agacgcagtc ccccggccgc gtcgaagtca cggcgtggcc tgtcggccga
     5221 cctgaccgcc cagaactggc cggtgaggcc ggcgagcagg agcgcaccga tggtgatcag
     5281 gatccgcctg ggagagacgt tcaagttcca cctccatcga atggtgcggg cttggaggct
     5341 gccccgctgg ttgcgcgcga gaccttgcga ccgggggctt gaacgatcct tgaacagacc
     5401 ccgcgcgtgg cgggacggcg gcggtgtaag acgctgctgc ggctggggtt aaggtttttc
     5461 tgcgaaaaaa ctcgcgcgcc ccttacgaag gccgcgcgca gcggtcactg cgggcgcggc
     5521 tcgacccgga tgaggctcgg aaggaccgag agcacgtcgc gcatcggcac cagggacaag
     5581 ccgccgttgc ggtccccgag gagccggaga aggaagcggg cggcggcgag gcgcggcacc
     5641 gatccggcgg gcgcgaagcg cgcgcccttg gacgagggga ccgtctcgat gaagccgcgt
     5701 tcgagcgcca cggcggcgta gccgcgcagc gcggggggaa tctgcgtgtc gtccttgagc
     5761 cccagcgcct cgccggcgcg cgcgtcggcc tcggccgcgc gcccggcggc gcgcaccatc
     5821 gcgaccgcga agtcgagacg atcgaccgcg tccgccggct tgaaccggag gggatcctcc
     5881 ggatcgatga gcacgacggc ggcgcgctgc cccgccgccg actcgacgaa cggaaaatcc
     5941 ggcgacgacg gcgggacatc ggtgaaggag gagagcgccg gcacccgctg cggccggtcg
     6001 gcgaccagcg cgagcgagcg ggccagctcg ccgcggcgca gcgacgcgga cggctcgaag
     6061 cgatcgccgc gcccgtcgag gacgtgccgg ctgacggcgc gcgccagcag gtcccgatcg
     6121 gtgctcccga gctgtccgag atcggtgaac ccggtcagca ccgccaccgc cgtctcctgc
     6181 cggatcgaga cggtccgatc ggcgggccca acccccaact tgtggacgat gcgcgcggtc
     6241 atcgtccgcg gcatggctcc ggccagctgc accccttccg tgtgggcgaa gagcaccgtg
     6301 gcgttgatcg actcggagcg gccgacctcc gcgccgtgct cgtcctcgag gatgagatcg
     6361 agatcgtcga gaccggccag ctcgtccccc cacgcgatcg acatccccca cgcggcggcc
     6421 gccgcgtcga gcgtgaccgg cagcgccagc gcgccgctcc aggggaccag gccggaggag
     6481 agcgccgcct ggtgctggta cgcgtaccgc cgctcgtcga gccagccggg gacgaaggtc
     6541 ccgaacgggc gcgcggcatc ggtggcctgc gtcagggcgg cccaggcctc gagccgcccc
     6601 gcccccgcct cggagcgctc gcgcgccagg atcggcgtcg cggtcgcctg caggatccgc
     6661 ttgagggcgg cgggggtcag cgcgggcgac gcctcgagca tcagcgccgc gacccccgcg
     6721 acgtgcggcg ccgcgaacga ggtgccgctc accgccgagt accagagcgc gtacgccggc
     6781 gccaccaccg ccccgccggc cgggtcggcg acgccgacga tgccgttgac catgccgacc
     6841 gccagcgggt tcgcggcgac gatcccctcg cccggcgcga ccagcgtcgg gtgcgagagg
     6901 atctcctcga agatgccgcg cgaagagaag ccggaaagcc ggccgtcgcg cccgaccgag
     6961 ccgaccccga tcacccacgg cgcgaccgcg tagggattca gcgtgtccgg ggcggggccg
     7021 tgattgccgg cggcaaagat caccgtgact ccttcgtcga agaggagacg ggtcgcgacg
     7081 ttcacgggat cggacgggtc gaagaacccg gacgtccccc aggagcagtt gacgatccgc
     7141 acgccgtacc gcgcgcggtt gtcgaggaga tagtcgaacc cctcgaggac cttgaggatg
     7201 aagacgtccc cggccgacag ggagaggatc gacgccgacg gggcgacccc cttgtacgtc
     7261 cccgcgctgg ccgcgccgct ccccgcggcg accgaggcca ccgccgtgcc gtggccgagg
     7321 acgaggtcgg tgttgatcaa cccctccacc gccgccgggt accggaaccc caacccgatc
     7381 cctccggtgc cgttgagccg gacgttgccg atcaccttgg agccgtaggg cagatcggga
     7441 tgggtgccgt ccacgccggt gtcgaggacc gcgatggtga cgcccgcgcc ggtgagaggg
     7501 gcgccaccgg gcgaccgcag cgccgcgtcg gtctcgacgt ccggcatgcc gatgaggtcg
     7561 cgactgtcgg cgtcgaagaa cgacagcgtc cgatcggcgt agagggagcg caccgccggc
     7621 agcaccgcga tggcgcggat ctgctccggc gtggcgtcca ccacgaccat cggcaggcgc
     7681 cggaacttcg tcccgccgag gatcccgagg gccgtcaggg tcgcgaggtc ggccgtcgac
     7741 ggcatcaggc ggtaggtcac gatgacgtcg atcgacgacg tgtccgggag cgtggagagg
     7801 aggttcagga gggccggatc gatcgagggg gcgtccggcg gctcgtcgaa ctgtagtccg
     7861 cccacgtcgt tccagcggat cccccccacg tcgttccagc ggatcccgga ggcgtcggtg
     7921 aacagcgtcc caccgacgtc gttccagcgg atgccgccga cgtcgttcca gcgcagaccg
     7981 ccgacgtcgt tccagcggat gccgcccacg tcgttccagc gcaggccgcc gacatcgttc
     8041 catcggatcc cggacgcctc ggtgacgtac ggaccggcgg gcaggagagt gacggacagg
     8101 aggaggcaga ccgccgccgc ggcgatccgc cgccggcctc cggccttcgc gtcacccgcg
     8161 ctgaataacg tggcgacctc ctgagtgtcg gacgagctcc ggctttcgaa aaagaattcc
     8221 tcaatgtacc acagcgcttc agcggcgacc ggccggaggc ggctccgccg ccagccgcag
     8281 ggcgcgctcg aacgcctggc gcgtcgaggt ggacgggacc gccgacaacc cgtcgaggag
     8341 cgccttgcgc agccgctcgt gctccttctc gatctcggcg cgccgccgca ggcgtccgag
     8401 cgccagcatc aggtcggcgg aggcggcctc gtcgatcgca tcctgggcga cggcacgccc
     8461 gagatacccg agcccggcgc cggcatcccc ccgctcgacg tgcaggcggc ccgcttcccg
     8521 cagcgccgca gccatcagga cctcgtaatg cgcccgcggc ccctcgatcc attcgtcgta
     8581 ctcctcctcg aggaacggcc cgcggtacag ggcgagcgcc gcgtcgtacg cggcgagcgc
     8641 cgccgtcgcg tcccccgccg cctgaagcct gcgcgcctcc cggatccccg cttcgaattt
     8701 ctcggcgtcg atgtcgtagc ggtacgccgg attgagacgg taggcgccgc gctcgaacag
     8761 gatgaagtgg cgcggcacgt tgtggccgtc gttgagcgca cgcctcagga acgagatcgt
     8821 cggatggaag ttcttctcga tgaccgcggg gcgcgcgtcc ccccagagcg cgtcgacgat
     8881 ccgctccttg gtggcgcgcc ggtcgcgcgc cacggcgagg taacagaaga tggtcagggc
     8941 gcgccggatc ttccaggcgc gcggcgggat cgcctgatcg cgatcgcgga acacctcgac
     9001 cggtccgaac aggcgcaccg ccaggtcggc gccgcccggg gccggggcgg accggcgggg
     9061 cgcctccatg ccgtcggcgc gcagcgcggc cggcgccccg gcgcgcgcgc cgagccagcg
     9121 ccggaagccg gggtcgagat cggcgacgcg caggagcgcc gcctcgtaat cgaagcgggc
     9181 ggagaggagc agcgccgtct ccgccgccct cccggccgga tcggccgccc cctggcggtg
     9241 ccgcgcgaag gccagccacg cgagcgccag gcaggaggga taggcgcgat cgagcccgga
     9301 gaagatcgcc gccgcctcct cgagcgccag gacggcccga tcgtcgcggc cggcgcgcac
     9361 ctccgtctcc cccgccgcca ggagcgccga ggcgagtccc tcgcggcggc cggcggcgcg
     9421 ggcccgctcc acgac
//
LOCUS       DASRIQ010000210        11873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_83904, whole genome shotgun sequence.
ACCESSION   DASRIQ010000210 DASRIQ010000000
VERSION     DASRIQ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83904"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFD06_05205"
     CDS             <1..934
                     /locus_tag="VFD06_05205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN02965.1"
                     /translation="LGVEVEEFRSTFRREFGNSWNEFRSRIRESRPDLVNVYAGSIGA
                     FAASLACPVPLVMTFGGPDLLGPAKKDRLSERGVGVMAVMFSQVAVPNADALVVRSPQ
                     LRGRLLRARDRRRAHVISTGVDTDLFRPCDRGMARREVGWPEQETIVLFGASRRRPIK
                     RFDRAVAAVERLSRRGLSARLESCEDVPPARMPLYLNAADCLLLTSQHEGSPNIVKEA
                     AACGCPVVSVPVGDVADILRGVTPGGLAAPDPESLAAGLYETLASRRRSNGPDRVRES
                     YALTLTARRVRQVYEDALSAWRSGRRHGWRVLHR"
     gene            941..1900
                     /locus_tag="VFD06_05210"
     CDS             941..1900
                     /locus_tag="VFD06_05210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN02966.1"
                     /translation="MTHERMPSGEGMAIVVVPRDRFSMFARCLDAIEARTPPPFRVVA
                     VAGGADPATRSYLEGLRTMRPHVTALLPDRLLEQSESRNLALRQVRERFVVVLENDTI
                     VQEGWLPPLLECLREERAAAVAPLLWWYRGLHAAGGSFEERDQDGAVAFDYRINYSGI
                     RRRRIDYPENHCVLIDRERLADFEFDEVEPFDVDLGLTLRQRGLSAFLEPRSLAHYEA
                     PPPLERRDLPPFRLRWDWTAWERSNLRFERKWGFVYDRACKRASYRRQRLKFGLAARY
                     PNALTLAAANLAFGATNRLQAAVAHRRWLRSGAARLFRLHATE"
     gene            1887..3074
                     /locus_tag="VFD06_05215"
     CDS             1887..3074
                     /locus_tag="VFD06_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN02967.1"
                     /translation="MRPNEPPPRLLLVANQAGDVLEYRLPLLDALRRAAFEVHLAVPP
                     EPAAAALAEHGYPVHAIPLRRLNVGPRDELRTLAALLRLYRSLRPALVHHLCLKPVLH
                     GGIAARLARVPSTIGTLTGLGPIFTRRSPDMRALRPLVSAGLRFACAGERHVLVVQNP
                     SDRDRLVASRIARPDRTVVIGGSGVDPTLFTPRPEPEGPPAVLLACRLLREKGVEDFV
                     AAAGRLRAGGHSARFVLSGEPVPEHPDSVPLAAIAALRDSGLLEWHGWSGDMPALFAR
                     SHIVCLPSYYGEGLPRVLLEAAASGRPIVTTDTPGCRDVVRHGWNGLLVPARDPEALA
                     GAIATLIEKPELRAVMGARGRAMVLAKFTLTRVIDEYVALHRAVIEAPGAAAAASPGV
                     IAR"
     gene            3181..3474
                     /locus_tag="VFD06_05220"
     CDS             3181..3474
                     /locus_tag="VFD06_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02968.1"
                     /translation="MRHKNLAFLLACLGFLTVAVASAPVPVESVPAPEQGVILSEPDA
                     CPPLPPATGEELTPVACRMMPECWSDSDCGAVCGTNGGRCVHSKCPVRICKCN"
     gene            complement(3499..6228)
                     /locus_tag="VFD06_05225"
     CDS             complement(3499..6228)
                     /locus_tag="VFD06_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN02969.1"
                     /translation="MTLPPGLRLGPYEVIAPLGAGGMGEVYRARDTRLARAVAIKILP
                     GRLASEPGARERFEREARTISQLSHPHICALYDLGHHEGLEYLVLEFLEGETLACRLE
                     RGSLPLEQTLRFGAQIASALGTAHQKGILHRDLKPANVMLTGAGVKLLDFGLAKALPG
                     PSDPEAAGELAALPTRTELTQEGRILGTVPYMAPEQIEGGRLDARTDIFALGAVLYEM
                     ATGKRAFEGKSSASLMASILEREPPPIASLRPQAPAAFDRLVRVCLAKRPDDRWQSAA
                     DVARELSWIAESKGEAPETSRAGAAARRGGKSRMAWVGLALLAAGALAGWLAARIGGG
                     WAARAPASGAASVERLSIVLPERAPLASASATPFAYLRNSLAVAPDDSRIACVSVTDD
                     GGTQIALRRADQFDPSPIPGTEGGFDPFFSPDGRWIGFFTFDSLRKVSLDGGAPLTLA
                     AIVNPAGGTWCDDGTIYVAGDEGTSLWRISAAGAASGGKAMQVALEGGAPLPAYPHCL
                     PGSKGLLMTMVERYEASDAAGEPVRFRQPLTSDHMSVGLLDLPEGRTPRPLVARGYAP
                     QYAASGHVVFARSGTLMAVPFDLARRAVTGPEAAMVNGVSMCSIALPTAQYAISPAGS
                     LLDVPGGDQARTRLVWVDRQGKIAPTAAGPDLFGSLKLSPDGKRAVVEVGGLQDDVYV
                     YDLGDGRRTRITSDGRSGRRGMWHPDGQRVIFYSKSDHHWVIKRVESDAAPELLAGIG
                     GFFTSWSAAGTLAGERSGRIWTGTLERQTAITDAGGEWGALVAPNGRLVAYTSSRTGI
                     YQVFAQPIPPTGQEWQVSADFGEEPIWSADSAELFYRRHSQWWSVPFAAGRPGSPRLL
                     FQGPFLNALGPSYDVAPDGRFLMALPPATGAIRVLQWTRNWTDELSLRLPQPR"
     gene            complement(6321..7793)
                     /locus_tag="VFD06_05230"
     CDS             complement(6321..7793)
                     /locus_tag="VFD06_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HZN02970.1"
                     /translation="MAIAAGALSLALPARAGEVALTIYNSDFALVKETRTLTLQQGAN
                     ETRFTNVTSLLEPDSVVLRDRKDPDGLRILEQNYEGDPLSQASMLRLSEGKTLQFRNV
                     NPATGAVEIVSGRLLRAGYGGGDGWAMRRHGGQPFGVAQTPMSPIVEMGGKVLFDLPG
                     QPLFEPPGKDAILKPTLLWRLQAGRGGERDVEVSYLTGGMRWEATYNLVAPEQGDAFD
                     LVGWITLENESGAEFQNARVKLMAGDVSKVQPGMAGMRKDMMVAMEAAAAPPVTEKAF
                     DEYHLYTLTNPTTLREREVKQVEFCRAARLPGKRIYVYDGALVPVYAYQGGMGQQPDL
                     GAQSNPKVATMLEFRNSKEAGLGIPLPKGTMKMYRADSDGSREFIGENSIDHTPADET
                     VRLTLGNAFDLRGERRQTDFKVDSSRQTATEAFEIKVRNHKKTAVEVRIVEHLYRWSG
                     WKIADSSVPYEKVDARTIEFKVTIPPDGEKVVTYRVNYSW"
     gene            7919..10315
                     /locus_tag="VFD06_05235"
     CDS             7919..10315
                     /locus_tag="VFD06_05235"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZN02971.1"
                     /translation="MLHHSHWTRPSGGSSRRVHRSSGSSRLTRSALLLLFAAVAQPGG
                     ASQAEPPELEKILDSVPTSESYGRHLKFLTEEPHQTGTARNMLLADYVRDRFVEYGLE
                     EVAFHDTPALMTYGRASSLTIVAPERIELRLVEEPHPPDKDSDLYSDPAHVPFHGYAP
                     SGDVQGEVVYANGGAPEDFALLDRMGVGVRGRIVLMRYSEPYSYRGTKIYEAERRGAI
                     GTILYSDPEDDGAGRGPVYPNGPWGPLSHMQWGAVLYDWLGPGEPFTFHWKQKKDGAW
                     VEGSKRDKQLPRLPSLPLSAGNAALILERLKGARGPESWQGGLPLPYHIGPGPVAVRL
                     RTVNEETIGTLRNVVGVLRGREEPDRLVILGNHRDAWIYGAVDPSSGTAALLETAKAL
                     GAASRRGLRPRRTIVFANWDGEEQLLGGSTQFAIDHRERLLREAVAYINVDSGVAGER
                     LEIGATPALAEFTRAIAAAVRDPRSGDSLLSAWEARSPERRAQVGMIVGATDYTAFQE
                     HLGISCIDMAFGGPYGVYHSMYDNYYWMSTIGDPGFEYGAALSRLWSLMAWRLADAPL
                     LPMRYSDYARKVQEHLTAIETKGSRERRLLRLDAAHAAARRWEEASVVFESAAPAQAT
                     GATERTSLRAVNDLLMQVERSLTEPKGLEGRPFYRHLIYAPQPTYREEILPRLWEAIE
                     RGDARKFPGHERELVAAFDRAAGLMIEARAALPAPVPTAAATREFRKDVLPILEGRCN
                     PCHFPGGVMHDRMPFEVEGTARVLGTRLFTRLKEPEEVAVIRAWLEQEAGAPRAEDPE
                     "
     gene            complement(10320..>11873)
                     /locus_tag="VFD06_05240"
     CDS             complement(10320..>11873)
                     /locus_tag="VFD06_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HZN02972.1"
                     /translation="LLGLTLLEAGEDPLDPTYADWLALSRDVYLAIPTPLADEYFGTS
                     DEALTDLLDGAPLDPDLLAAIDALPPGLRAPVSEAVATGSMSMQRGALPDGLARSRRG
                     GISPEVLPADLPGLVGDVNAAAAATAVLYGRVVARGERGPFGGGDTFGSGALYRAIFR
                     GLVAARAGDVAALGDIAAGAEQAAQAVAEARLLAASVAPQAVQHLRDGVAVLAAALDA
                     ADGDPADLQVLGTRLAELVYRIDRAAGNSAALAALEAGAADFLLPDLSAPVVTAAPAG
                     PLFTGSVVVTLTSDEPATIFYTRDGSDPVPGGSATFSGAHQVAISITSDTVLSWLGRD
                     PAGNQGSVVRITYRNDGDADGLADIADNCPLVANAGQENFDGDAQGDVCDPDDDNDTF
                     ADFADCRPLDPTLWAAPGESPITLTFIDADTLHWTSIAAAAGPATTYDGARGSLADLR
                     TQPPGSEFSTAVCLFDDLLNPALETDDATLPAVGQGFYYFLRGDNLCGMGSYGRGSGG
                     AERVLLACP"
BASE COUNT         1681 a         4277 c         4168 g         1747 t
ORIGIN      
        1 cctcggggtc gaggtggagg agttccgcag caccttccgg agggagttcg gcaacagctg
       61 gaatgagttc cgctcccgca tccgcgagag ccgccccgac ctggtcaatg tctacgcggg
      121 atcgatcggc gccttcgccg catcgctggc ctgcccggtc ccgctggtca tgaccttcgg
      181 gggtcccgat ctgctgggcc ccgcgaagaa ggaccggctc tccgagcgcg gtgtcggcgt
      241 catggcggtg atgttctccc aggtggccgt gccgaacgcc gacgcgctgg tggtgcgctc
      301 gccccagctt cgcggccgtc tcctgcgggc ccgcgaccgg cggagggctc acgtgatttc
      361 cacgggcgtg gacacggatc tgttccggcc ctgcgaccgg gggatggcca ggcgcgaggt
      421 cggctggccc gaacaggaga cgatcgtcct gttcggggcg tcccgccgcc gtcccatcaa
      481 gcgcttcgac cgcgccgtcg ccgcggtcga gcgcctcagc cgccgcggcc tctccgcccg
      541 cctggagtcc tgcgaggacg tccctcccgc gcgcatgccg ctgtatctga acgcggccga
      601 ctgcctcctg ctgacgagcc agcacgaggg ctcccccaac atcgtcaagg aggccgccgc
      661 ctgcggctgt ccggtggtct ccgttcccgt cggcgacgtg gccgacatcc tccggggcgt
      721 caccccgggg ggcctggccg ctcccgaccc ggagtcgctc gcggcggggc tgtacgagac
      781 gctggcgtcc cggcgtcgat ccaacggtcc cgatcgcgtc cgggagtcgt acgccctgac
      841 gctcacggcg cgccgggtgc ggcaggtcta cgaagacgcg ctgtccgcct ggcgctcagg
      901 acgacgccac ggctggcgcg tcctgcaccg ctgaggccgc atgacccacg agcgcatgcc
      961 gtcgggcgaa gggatggcga tcgtcgtcgt cccgcgcgat cgtttcagca tgtttgcgcg
     1021 ctgcctcgac gcgatcgagg cgcggacgcc gccgccgttc cgcgtcgtcg cagtcgccgg
     1081 cggcgcggat ccggccaccc ggtcgtacct cgaagggctg cggaccatgc ggccccacgt
     1141 gaccgcgctg ctcccggacc ggctcctcga gcagagcgag tcgcgcaacc tggccctgcg
     1201 gcaggtccgc gagcgcttcg tcgtcgtcct ggagaacgac acgatcgtgc aggaggggtg
     1261 gctccccccc ctcctcgagt gcctgcggga agagcgggcc gcggcggtcg cgccgcttct
     1321 ctggtggtac cgcggcctgc acgccgccgg cggctccttc gaggagcggg accaggacgg
     1381 cgccgtcgcg ttcgactacc ggatcaacta ctcggggatc cggaggaggc ggatcgacta
     1441 tccggagaac cactgcgtcc tcatcgatcg ggagcggctc gccgactttg agttcgacga
     1501 ggtcgagccg ttcgacgtcg atctcggtct gacgctccgg cagcgcgggc tcagcgcctt
     1561 tctcgagcct cgctcgctgg cgcactacga ggctccgcct cctctggaga ggcgcgatct
     1621 ccctccgttc aggctgcgct gggactggac ggcctgggag cggagcaacc tccggttcga
     1681 gcggaaatgg ggattcgtct acgaccgggc ctgcaagcgg gcctcgtacc gccgacagcg
     1741 gctcaagttc gggctcgcag cccggtaccc gaacgcactg acactcgccg cggcgaacct
     1801 ggccttcggc gcgaccaacc gactccaggc ggcggtggcg caccgtcgct ggctgcgctc
     1861 cggcgccgcg cgcctgttcc gactccatgc gaccgaatga acccccgccg cggctgctcc
     1921 tcgtcgccaa ccaggccggc gacgtcctgg agtaccgtct gcccctgctc gacgccctgc
     1981 ggcgggcggc cttcgaggtc cacctggcgg tcccgccgga gcccgccgcg gcggccctcg
     2041 cagagcatgg ataccccgtt cacgccatcc cactccgccg gctgaacgtc ggcccccggg
     2101 acgagctgcg cacgctcgcc gcgctgctcc gtctctaccg gagtctgcga ccggcactcg
     2161 tgcaccatct ctgtctcaag ccggtcctgc acggcgggat cgccgcgcgg ctggcccggg
     2221 tgccttccac gatcggcacc ctgaccggtc tgggcccgat tttcacgaga cgctcgccgg
     2281 acatgcgggc cctgcgccca ctggtctcgg cggggctccg gtttgcctgc gcgggcgagc
     2341 ggcacgtcct ggtcgtgcag aatccctccg atcgcgatcg cctcgtcgcg agccgcatcg
     2401 cccggccgga tcgcacggtg gtcatcggag gctccggggt ggatccgacg ctgttcacgc
     2461 cccgcccgga gcccgaaggg ccgcctgcgg tcctgttggc ctgcaggctg ttgcgcgaaa
     2521 aaggggtcga ggacttcgtg gcggcggccg ggcggctgcg cgccggcgga cactcggcgc
     2581 gcttcgtcct gtccggcgaa ccggtgcccg aacacccgga cagcgtcccg ctcgcggcga
     2641 tcgcggcctt gcgcgactcc ggacttctgg agtggcacgg gtggagcggc gacatgccgg
     2701 cgctgttcgc ccggagccac atcgtctgcc tgccttccta ctacggcgaa gggcttcccc
     2761 gcgtcctcct cgaggcggcc gccagcggcc gccccatcgt gaccaccgac actccgggat
     2821 gccgggacgt ggtgcgccac ggatggaacg gcctgctcgt gccggcacgc gaccccgagg
     2881 ctctcgccgg ggccatcgcc accctgatcg agaagccgga gctgcgagcc gtcatggggg
     2941 cgcgcggccg cgcgatggtg ctggcgaaat tcacgctcac gcgggtgatc gacgagtacg
     3001 tcgcgctcca tcgcgcggtg atcgaagcgc cgggagcggc cgcggcagcc tcaccaggag
     3061 tcatcgcccg gtaggtgctg accttctctc tgcgacccgt cgatcgacga tctcgactgc
     3121 cccgttgcgc agccggccag agccgattat tctcgcggtc accttccgga ggaggaaacc
     3181 atgaggcaca agaatctggc gtttctcctg gcgtgtctgg ggttcttgac cgtcgctgtg
     3241 gcgagcgcac cggtccccgt cgaaagcgtg ccggctcccg agcagggcgt cattctcagc
     3301 gaacccgacg cctgcccccc gctcccgccc gcgaccgggg aagagctcac tccggtggcg
     3361 tgccggatga tgcccgagtg ctggagcgac agcgactgcg gcgctgtgtg cggcacgaac
     3421 ggcggccgct gcgtccacag caagtgcccg gtccgcatct gcaagtgcaa ctgaccggcc
     3481 ttccgcgctg agaagcgctc agcgcggctg cggcaggcgg aggctcaact cgtcggtcca
     3541 gttccgggtc cactgcagca cgcggatcgc gccggtcgcg ggcggaaggg ccatgaggaa
     3601 acggccgtcc ggggcgacgt cgtacgacgg gccgagtgcg ttgaggaacg gcccctggaa
     3661 gagaagacgc ggcgaacccg gtcgtcccgc cgcaaagggg acgctccacc actggctgtg
     3721 ccggcggtag aacagctcgg ccgagtcggc ggaccagatc ggctcctctc cgaaatcggc
     3781 cgagacctgc cactcctgtc cggtcggcgg aatgggctgc gcgaagacct ggtagatgcc
     3841 ggtgcgtgat gaggtgtagg cgacgagtct gccattcggc gcgaccaggg ccccccattc
     3901 tccgccggcg tcagtgatcg cggtctggcg ctcgagggtc ccggtccaga ttcgcccgct
     3961 ccgctctccc gcgagcgttc cggccgccga ccaggaggtg aaaaacccgc cgatgccggc
     4021 gagcaactcg ggggccgcat cgctctcgac ccgtttgatc acccaatggt ggtcgctctt
     4081 ggaataaaag atgacccgtt gtccgtcggg atgccacatg ccacgccggc cgctcctgcc
     4141 gtcgctcgtg atgcgggtgc gccggccatc cccgagatcg tagacataga cgtcgtcctg
     4201 caggccgccg acctccacga ccgcgcgctt tccgtcggga gagagcttga ggcttccgaa
     4261 cagatcaggg ccggcggcgg tcggcgcgat cttcccctgg cgatccaccc agacgagccg
     4321 ggtgcgcgcc tggtcgccgc cggggacatc gagcagtgag ccggccgggc tgatggcgta
     4381 ctgcgcggtc gggagcgcga tcgagcacat ggagacaccg ttcaccatcg ccgcctcggg
     4441 accggtcacc gcgcggcgcg ccaggtcgaa cgggacggcc atgagcgtgc cgctgcgggc
     4501 gaagacgacg tgtcccgacg cggcgtactg cggcgcgtag ccgcgcgcca cgagcggacg
     4561 cggggtccgg ccctcaggga ggtcgagcag acccacagac atgtggtcgg aggtcagcgg
     4621 ctgccggaag cgcaccggct ctcccgctgc gtcggacgcc tcgtaccgtt cgaccatcgt
     4681 catcagcagg cccttgcttc ccggcaggca gtgcgggtac gccggcagcg gggcgccgcc
     4741 ctcgagcgcc acctgcatcg ccttgccgcc cgaagccgcg ccggccgccg agatacgcca
     4801 caggctggtc ccctcgtccc ccgcgacata gatcgtgccg tcgtcgcacc acgtccctcc
     4861 ggccgggttg acgatggcgg ccagcgtcag cggggcgccg ccatcgagcg acaccttgcg
     4921 cagcgagtcg aaggtgaaga agccgatcca acgcccgtcg ggtgaaaaga acggatcgaa
     4981 tcccccctcc gtcccgggga tcggcgaggg atcgaactga tccgcccgtc gaagagcgat
     5041 ctgcgtgccg ccgtcatccg tgacggagac acaggcgatg cgggagtcgt ccggggcgac
     5101 cgccagggaa ttcctcaggt aggcgaacgg ggtggcggag gccgacgcca gcggggcgcg
     5161 ttccggcagc acgatggaca ggcgctcgac gcttgcggcg ccggatgcgg gcgcgcgggc
     5221 cgcccagccg ccccctatcc tcgcggccag ccacccggca agcgcgcccg cggcgagcaa
     5281 cgccagcccc acccacgcca ttcggctttt ccccccgcgg cgcgccgcgg ccccggcgcg
     5341 cgaggtctcg ggcgcctccc ccttcgactc ggcgatccac gacagctccc gggcgacgtc
     5401 ggcagcgctc tgccagcgat cgtcgggacg cttcgcgaga cagacgcgca ccagccggtc
     5461 gaaggccgcc ggcgcctgtg gtcgcaagga agcgatcggg ggcggctcgc gctcgaggat
     5521 cgaggccatc aggctggccg agcttttccc ctcgaacgct cgtttccccg tggccatctc
     5581 gtagagcacc gccccgagcg cgaagatgtc ggtgcgcgcg tccagccgcc ccccctcgat
     5641 ctgctcggga gccatgtacg ggaccgttcc gagaatcctc ccctcctggg tcagctcggt
     5701 gcgggtcgga agcgcggcga gttcgccggc ggcctccgga tccgagggcc ccggcaacgc
     5761 cttcgccagc ccgaagtcga ggagcttcac tccggcgccg gtgagcatca cgttcgcagg
     5821 cttcagatcg cgatggagga ttcccttctg gtgggccgtc ccgagcgccg aagcaatctg
     5881 cgctccgaag cgcaaggttt gttcgagcgg cagactcccc cgctccagac gacacgcgag
     5941 tgtctcccct tcgaggaatt cgaggacgag atactcgaga ccctcgtggt gcccgaggtc
     6001 gtacaaagcg cagatgtgcg ggtgggaaag ctgcgagatg gttctcgcct cccgctcgaa
     6061 acgctcacgc gcgccgggtt cggaggcgag acgcccgggg aggatcttga tggcgaccgc
     6121 gcgcgccagc cgcgtgtcgc gcgcccggta gacctccccc atcccgcctg ctccaagcgg
     6181 cgcaatgact tcgtacggcc ccagacgaag gccgggcggc agagtcattc gaggtcgtgg
     6241 cgctgacggc ggatcgacgg ctcccgcgtt ggaacgtcgc tgcctatctt acgtcgggcc
     6301 caggcctggc gccttcgctc tcaccaggag taattcacgc ggtacgtgac gaccttctca
     6361 ccgtcggggg ggatcgtgac cttgaactcg atggtgcggg cgtccacctt ttcgtaaggg
     6421 acggaggagt cggcgatctt ccagccgctc cagcggtaga ggtgctcgac gatgcgcacc
     6481 tcgaccgcag tcttcttgtg gttgcgcacc ttgatctcga aggcttcggt cgcggtctgg
     6541 cgcgacgaat cgaccttgaa gtcggtctgg cggcgctcgc cgcggaggtc gaaggcgttg
     6601 ccgagcgtca ggcggaccgt ctcgtcggcg ggggtgtggt cgatgctgtt ctcgccgatg
     6661 aactcgcgcg agccgtcgct gtcggctcgg tacatcttca tcgtcccctt cggcagcgga
     6721 atgccgaggc ccgcctcctt gctgttgcgg aactcgagca tggtcgcgac cttggggttc
     6781 gactgcgcgc cgaggtcggg ctgctgcccc atccccccct ggtaggcgta caccgggacg
     6841 agcgccccgt cgtagacgta gatgcgcttg ccggggaggc gcgcggcgcg gcagaactcc
     6901 acctgcttga cctcccgttc gcgcagcgtc gtcgggttcg tgagcgtgta caggtggtac
     6961 tcgtcgaacg ccttttccgt gaccgggggc gccgccgcgg cttccatcgc caccatcatg
     7021 tccttgcgca tgccggccat ccccggctgc accttgctga cgtcccccgc catcagcttc
     7081 acgcgggcgt tctggaactc ggcgccgctc tcgttctcga gggtgatcca gccgacgagg
     7141 tcgaaggcgt ccccctgctc cggcgccacc aggttgtagg tcgcctccca gcgcatcccg
     7201 ccggtcagat acgacacctc gacgtcgcgc tcgccgccgc gccccgcctg cagccgccat
     7261 aggagcgtcg gcttcaggat ggcgtccttc cccggcggct cgaacagcgg ctgccccggg
     7321 aggtcgaaca gaaccttgcc gcccatctcg acgatcggcg acatcggcgt ctgcgccacg
     7381 ccgaagggct ggccgccgtg ccggcgcatc gcccagccgt cgccgccacc gtagccggcg
     7441 cggaggagcc ggccgctgac gatctccacg gcgccggtgg ccgggttgac gttgcggaac
     7501 tgcagcgtct tcccttccga gaggcgcagc atcgacgcct ggctcagcgg atccccctcg
     7561 tagttctgct cgaggatgcg gaggccatcg gggtcttttc gatcacggag gaccaccgag
     7621 tccggctcga ggagcgacgt gacgttggtg aagcgggtct cgttcgcccc ctgctgcagg
     7681 gtgagcgtgc gggtctcctt cacgagggcg aagtcgctgt tgtagatggt cagcgccacc
     7741 tcgccggcgc gggccggcag cgccagcgac agcgcgcccg cggcgatcgc catcctcatg
     7801 gaccgtacgt gaagaccccg catcgttgct cctttcgtgt gcctggaccc cttccgcggt
     7861 acgatgccgc gcccggcgcg tctggccgcc gggtggcggc atcttagagg aaggacgctt
     7921 gctccaccac tcccattgga cccggccaag cggcgggagt tcccgccgcg ttcatcgaag
     7981 cagcgggagt tcccgcctga cgagaagcgc gctcctgctt ctcttcgccg ccgtcgccca
     8041 gccgggcggg gcctcgcagg ccgagccgcc cgagctggag aagatcctcg actccgttcc
     8101 cacatccgag tcctacggcc ggcacctgaa gttcctgacc gaggagccgc accagaccgg
     8161 cacggcgcgc aacatgctgc tcgcggacta cgtgcgcgac cgattcgtcg agtacggact
     8221 cgaagaggtc gccttccacg atacgccggc cctgatgacc tacggtcgcg cgtcgagcct
     8281 cacgatcgtc gcccccgaaa ggatcgagct gcggctcgtc gaggagccgc acccgcccga
     8341 caaggactcc gacctgtact ccgacccggc gcacgtgccg ttccacggct atgccccctc
     8401 gggagacgtg cagggggagg tggtgtacgc caacggcggc gccccggagg acttcgcgct
     8461 gctcgatcgc atgggggtgg gggtgcgcgg ccgcatcgtc ctgatgcgct actcggagcc
     8521 ctattcctac cgcgggacca agatctacga ggccgagcgc cgcggcgcga tcgggacgat
     8581 cctctactcg gatccggagg acgacggtgc cgggcgcggt ccggtctatc cgaacggccc
     8641 gtggggaccc ctcagccaca tgcagtgggg agcggtcctg tacgactggc tcggccccgg
     8701 cgagccgttc accttccact ggaagcagaa gaaggacggc gcctgggtgg agggatcgaa
     8761 gcgcgacaag cagctgccgc gcctgccatc gctgccgctc agcgccggca acgccgccct
     8821 cattctcgag cggctcaagg gggcgcgggg accggagtcg tggcaggggg gacttcccct
     8881 cccctaccac atcggcccgg ggccggtcgc ggtgcgcctc cgcaccgtca atgaggagac
     8941 gatcggcacg ctgcgcaacg tcgtcggcgt gctgcgcggg cgcgaggagc cggaccggct
     9001 ggtgatcctc gggaaccacc gcgacgcctg gatctacggc gcggtcgacc cctcgagcgg
     9061 caccgcggcg ctcctcgaga cggcgaaagc gctcggggct gcgagccggc gcggcctccg
     9121 gccgcgccgc acgatcgtct tcgccaactg ggacggcgag gagcagctcc tcggcggttc
     9181 gacgcagttc gccatcgacc atcgcgagcg gctgctcagg gaggcggtcg cgtacatcaa
     9241 cgtggacagc ggcgtcgccg gcgagcggct cgaaatcggg gcgacgccgg cgctcgcgga
     9301 gttcacgcgc gccatcgcgg cagccgtccg cgatccccga agcggcgact cgctcctctc
     9361 cgcgtgggag gcgcgctccc ccgaacggcg cgcccaggtc ggcatgatcg tcggcgccac
     9421 cgactacacc gccttccagg agcacctcgg catctcctgc atcgacatgg cgttcggcgg
     9481 cccctacggc gtctaccact cgatgtacga caactactac tggatgagca ccatcggcga
     9541 cccgggcttc gagtacggcg cggcgctgtc gcgcctctgg agcctcatgg cctggcggct
     9601 cgccgacgcg ccgctcctgc cgatgcgcta ttcggactac gcccgcaagg tgcaggagca
     9661 cctcaccgcg atcgagacga aaggctcgcg cgagcgccgc ctgctccgcc tcgatgcggc
     9721 ccacgccgcc gcgcgccgct gggaggaggc ttccgtcgtc ttcgagtcgg ccgcaccggc
     9781 gcaggccacc ggcgcgacgg agcgaacctc actgcgggcg gtgaacgatc tcctgatgca
     9841 ggtggagcgg tccctgaccg agccgaaagg cctcgaaggg cggccgttct accggcacct
     9901 catctatgcg ccgcagccga cctaccgcga agagatcctg ccgcgcctct gggaggcgat
     9961 cgagcgcggc gacgcgcgga agttccccgg ccacgagcgg gagctggtcg cagcgttcga
    10021 ccgggcggcc gggctgatga tcgaggcgcg cgccgcgctc cccgcgccgg tccccacggc
    10081 tgcggcgacg cgcgagttca ggaaggacgt cctgccgatc ctcgaggggc gctgcaaccc
    10141 gtgccatttt ccgggcgggg tgatgcacga tcgcatgccg ttcgaggtgg aagggacggc
    10201 gcgcgtcctc ggaacgcggc tgttcacgcg cctgaaggaa ccggaggagg tggcggtgat
    10261 ccgcgcctgg ctcgagcagg aggccggagc gccgcgcgct gaggatcccg agtgatcgat
    10321 cagggacagg cgaggaggac ccgctccgct cctcccgaac cacggccgta ggatcccatg
    10381 ccgcagaggt tgtccccgcg caagaagtag tagaacccct gccccacggc cggaagggtc
    10441 gcgtcgtcgg tctcgagcgc cgggttcagc agatcgtcga agaggcagac cgccgtcgag
    10501 aactcgctcc cgggcggctg cgtccggagg tcggccaaag agccgcgcgc gccgtcgtag
    10561 gtggtggccg ggcccgccgc ggccgcgatg ctcgtccagt gcagcgtgtc ggcgtcgatg
    10621 aaggtcaggg tgatcggcga ttcgccgggg gcggcccaca gagtggggtc gagcggccgg
    10681 cagtcggcga aatcggcgaa ggtgtcgttg tcgtcgtccg gatcgcagac gtccccctgc
    10741 gcgtcgccgt cgaagttctc ctgtccggca ttggcgacga gcgggcagtt gtcggcgatg
    10801 tcggcgagcc cgtcggcgtc gccgtcgttc cggtaggtga tgcggacgac cgagccctgg
    10861 ttgccggccg gatcgcggcc gagccaggac aggaccgtgt ccgacgtgat ggagatcgcg
    10921 acctggtgcg cgcccgagaa ggtcgcggac ccgcccggaa ccggatcgga tccatcccgc
    10981 gtgtagaaga tggtggccgg ctcgtcggac gtgagcgtga ccacgaccga gcccgtgaac
    11041 agcggccccg ccggcgcggc ggtcacaacg ggggccgaca ggtccggcag caggaagtcg
    11101 gccgcccccg cctcgagcgc cgccagcgcc gcggagttcc cggcggcgcg atcgatccgg
    11161 tacaccagct cggcgagccg ggtgccgagc acctgcaggt cggcgggatc gccgtcggcg
    11221 gcgtccagcg cggcggccag gaccgcgacc ccgtcgcgca gatgctgcac ggcctggggc
    11281 gcgacggagg cggcgaggag ccgcgcctcg gccaccgcct gcgccgcctg ctcggcaccg
    11341 gcggcgatgt cgccgagcgc cgcgacgtcc cccgcccgcg ccgcgaccag cccccggaag
    11401 atggcgcgat agagtgcccc ggacccgaac gtgtcgccgc ccccgaaagg cccgcgctcg
    11461 ccgcgcgcga cgacgcggcc gtagagaacc gccgtggccg ccgccgccgc attgacgtcc
    11521 ccgaccagcc ccgggagatc ggccggcagg acctcggggc tgatgccgcc gcggcgggat
    11581 cgggcgagcc cgtccggcaa cgcaccccgc tgcatcgaca tcgatcccgt cgccaccgct
    11641 tcggagacgg gagcgcgcag ccccggcggc agggcgtcga tcgccgcgag cagatccgga
    11701 tcgagggggg cgccgtcgag caggtcggtc agcgcctcgt cgctcgtccc gaagtactcg
    11761 tccgcgagcg gcgtcgggat cgcgaggtag acgtcgcggc tcagcgccag ccagtcggcg
    11821 taggtcggat cgagcgggtc ctccccggct tcgagcagcg tgagaccgag gag
//
LOCUS       DASRIQ010000211         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3751701, whole genome shotgun sequence.
ACCESSION   DASRIQ010000211 DASRIQ010000000
VERSION     DASRIQ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3751701"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VFD06_05245"
     CDS             <1..135
                     /locus_tag="VFD06_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015428748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02973.1"
                     /translation="KCYGGDITRKRKLLEKQKEGKKRMKKVGVVDIPQEAFLALLKVD
                     "
     gene            159..908
                     /gene="lepB"
                     /locus_tag="VFD06_05250"
     CDS             159..908
                     /gene="lepB"
                     /locus_tag="VFD06_05250"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZN02974.1"
                     /translation="MPDLPAPPQARPVPAVAPHPPRRWLFDYAQAAVVALIFALFVRT
                     YLFQVFKIPSASMEDTLLVGDHLVVNKFSLAPLALPAERGILPLGEVRRGDIVIFRYP
                     HDPRQDYIKRVIGLPGDSLKIVDQVVWVQTRGQTGYAPLSEPYAVHKYPGGVPDSLNN
                     YGPVEVPDGEYFMMGDNRDDSLDSREWGFVPRRLIVGRPLVIYWSFDGIAPDGALRLA
                     AEDDSGWRRLLHSFTAIFRMTRWERSGRMVR"
     gene            complement(928..1332)
                     /locus_tag="VFD06_05255"
     CDS             complement(928..1332)
                     /locus_tag="VFD06_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02975.1"
                     /translation="MASTSSRVTQQPDTRSRWSERGEGRLGTIFGLVVLAVTVYLAFK
                     VVPVMVNAYAFRDYIEQETRFAALRNRDEEVIKRVLRKAEELDLPVTRKSITVNRSTS
                     HFDIRVAYTIPIETPVYTYNWIFKEEYRAPLF"
     gene            complement(1543..>2773)
                     /locus_tag="VFD06_05260"
     CDS             complement(1543..>2773)
                     /locus_tag="VFD06_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02976.1"
                     /translation="AGGGGAGAWGFWDRLAPFQNVYDHISYIWSAFGVSPTYCVLTPT
                     DTGSGGFAPRGVKETLCYPNGVWSHCGNSRDGTNTGQCPGPGENGTSGPGLWVQSKFS
                     LANFLGQTVRIRWIAEAWEFDCCASSYYELGGAWAPQTGDEGWWIDDIRVQGALQASA
                     LPLPDLKAPGPGACPATACNAASGDGGFAMDLTVTEADLDTLVVGGESIVLSAAATLN
                     TGGCVGGGVQYRFFKNGTTNADVVQDWSSDPTFVDNPMSDAVYRVQARCSIFPACSST
                     IATGASTETIQVYSGDSQDINLTLTHAAGTTTIQWLSRPQSVLVSGYDYFAGGTINTS
                     GDVGQNTLAGLACGTGNMAQVLPLGTPITRTDGVTPAVGTARYFLVGHSPLAAGGQAA
                     LGRRSNGALRPLGPVCP"
BASE COUNT          431 a          977 c          936 g          429 t
ORIGIN      
        1 aagtgctacg gcggcgacat cacccgcaag cgcaagctcc tcgagaagca gaaggagggg
       61 aagaagcgca tgaagaaggt cggcgtcgtg gacatccctc aagaagcatt cctcgccctc
      121 ctgaaagtgg actaggacga ggggagcgga gcgccctcgt gccggacctc cccgcccccc
      181 ctcaggcacg tccggtcccc gcggtggcgc cgcacccgcc gcggcgctgg ctcttcgact
      241 acgcgcaggc ggcggtcgtc gccctgatct tcgccctctt cgtgcggacg tacctcttcc
      301 aggtcttcaa gatcccgagc gcctcgatgg aggacacgct cctcgtcggc gatcatctgg
      361 tggtgaacaa gttctcgctc gccccgctgg ccctcccggc cgagcgcggc atcctgccgc
      421 tgggcgaggt gcggcggggc gacatcgtca tcttccgcta cccgcatgat ccgcggcagg
      481 actacatcaa gcgggtgatc ggcctgccgg gggacagcct gaagatcgtc gaccaggtcg
      541 tctgggtgca gacgcgcggc cagaccgggt acgcgccgct cagcgagccg tacgcggtgc
      601 acaagtaccc gggaggggtg ccggacagcc tgaacaacta cggtccggtc gaggtccccg
      661 acggggagta cttcatgatg ggagacaacc gcgacgacag cctcgacagc cgcgagtggg
      721 ggttcgtccc gcgccgcctc atcgtcggcc ggccgctcgt gatctactgg tccttcgacg
      781 gcatcgcgcc ggacggcgcg ctccggctcg ccgcggagga cgactccggc tggcgccgcc
      841 tgttgcactc cttcacggcg atcttccgga tgacgcgctg ggagcgatcg ggacggatgg
      901 tgcgctgaga cgggcgggag ggcgccctca gaagagcggg gcgcggtact cctctttgaa
      961 gatccagttg taggtgtaga ccggggtctc gatcgggatc gtgtaggcga cccggatgtc
     1021 gaagtgcgag gtgctgcggt tgaccgtgat gctcttgcgg gtcaccggca ggtcgagctc
     1081 ctccgccttc ctgagcaccc gcttgatgac ctcctcgtcc ctgttgcgca gcgccgcgaa
     1141 gcgcgtctcc tgctcgatgt agtcgcggaa ggcgtaggcg ttcaccatca ccggcaccac
     1201 cttgaacgcc aggtacaccg tcaccgccag caccaccagc ccgaagatcg tccccagccg
     1261 accctcgccg cgctccgacc acctcgagcg cgtgtcaggc tgttgcgtga ccctcgaact
     1321 cgtggacgcc atcgaagtct ctcccggccg cacccgcagc cgaccccaat ataggaaccg
     1381 caccccctcc acgccactcg tggcgccgca cccacccgac ccgattacga ccccgtaatc
     1441 gccacgcggc gaccgcgtgc cccgccggcc tcacctcccg ttcaggcgta aaaaaagggg
     1501 ccccggtttc ccggggcccc tcgttctttt cgcgttacga agctacggac aaaccggtcc
     1561 gagcgggcgc agcgccccgt tcgaacggcg tccgagagcc gcctgaccgc cggccgcgag
     1621 cgggctgtgg ccgacgagga agtaccgtgc cgtgccgacc gccggggtga cgccgtcggt
     1681 ccgggtaatc ggcgtgccga gcggcagcac ctgcgccatg ttccccgtac cgcaggcgag
     1741 accggccagc gtgttctgcc caacatcccc ggaggtgttg atggtgccgc cggcgaagta
     1801 gtcgtagccg gagaccagca ccgactgcgg ccgcgacagc cactggatcg tggtggtccc
     1861 ggcggcatgg gtcagggtca ggttgatgtc ctgactgtcg cccgagtaga cctggatcgt
     1921 ctcggtcgac gcccccgtcg cgatggtgct ggagcaggcc gggaagatgc tgcagcgcgc
     1981 ctgcacgcgg tagacggcgt cggacatcgg attgtcgacg aaggtcgggt ccgatgacca
     2041 gtcctgcacc acgtcggcgt tggtcgtgcc gttcttgaag aagcggtact gcactccgcc
     2101 tccgacgcag ccgccggtgt tgagagtcgc ggcggcggac aggacgatcg actctccgcc
     2161 gacgaccagg gtgtcgaggt cggcttcggt caccgtcagg tccatggcga agccgccgtc
     2221 gccgctggcc gcgttgcagg cggttgccgg gcaagcgccc ggacccggtg ctttgagatc
     2281 cggaagcggc agcgccgagg cctgcagcgc cccctggacc cggatgtcgt cgatccacca
     2341 gccctcgtca ccggtctgcg gggcccaggc gccgcccagc tcgtagtacg acgacgcgca
     2401 gcagtcgaac tcccaggctt cggcgatcca gcggatgcgg accgtctggc cgaggaagtt
     2461 ggcgaggctg aacttgctct ggacccacag gcccggaccg ctcgtgccgt tctcgcccgg
     2521 cccggggcac tgcccggtgt tggtgccgtc gcgcgagttg ccgcagtgcg accagacccc
     2581 gttcgggtag cacagggttt ccttgacgcc gcgcggcgcg aagccgccgc tgccggtgtc
     2641 ggtcggggtc aggacgcagt acgtcggcga cacgccgaac gccgaccaga tgtaggagat
     2701 gtggtcgtag acgttctgga acggcgccag ccgatcccag aagccccacg cccccgcccc
     2761 gccgccgccg gcc
//
LOCUS       DASRIQ010000212         7220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1902312, whole genome shotgun sequence.
ACCESSION   DASRIQ010000212 DASRIQ010000000
VERSION     DASRIQ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902312"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFD06_05265"
     CDS             <1..495
                     /locus_tag="VFD06_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZN02977.1"
                     /translation="IESRRAVVEVEQYHYATSQLAQTTLRSVLGQVELDDLLSQRERL
                     NADLQQILDRHTDPWGIKVSMVEVKHVDLPPEMQRAMAKQAEAEREKRAKIIHADGEL
                     QASASLAKAADIISANPTTLQLRYLQTLTEIAAEKNSTIIFPMPIELFQGLKHALGPA
                     RDRA"
     gene            492..1541
                     /locus_tag="VFD06_05270"
     CDS             492..1541
                     /locus_tag="VFD06_05270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZN02978.1"
                     /translation="MSEAPAPLADQLDVAQRALEVCRCIEPQPAAEAFLRGAVSYTRL
                     PVGALYLYDAESDMFRLAVSTLPDGAAQREEIASLDLAALRRSGPPETWGPAVVSPPL
                     PLDLLGSDPIVAVPLTGSGRLLGMLVLGDVAGGAAAPEPLARLRRLLPEMLPGLLNAH
                     RVQAYRDLVIKDDQSDCYNRRFFDRCLSEEVYRAQRYGQALSLVFLDLDNLKQVNARY
                     GHAAGSRTVREVSRRLLGTIRGSDRAFRYGGDEFCLVLPATDLTGARELCERLRVAIG
                     GRPFVVDTAAHLTVTASFGIATYPLHARTSLGLIKCADEAMQTAKAAGKNLVYVAGET
                     APSPSPRAKAAAERR"
     gene            1538..2050
                     /locus_tag="VFD06_05275"
     CDS             1538..2050
                     /locus_tag="VFD06_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZN02979.1"
                     /translation="MSSTRRAGRPIVAVLVTSVLMLAGARFIGRVAASRAPAAGAGGD
                     AATQQAAAVPDLTFYSTLGDASPPRGKGGAKGTGEAALRAAPGDIVPAEGIYVVQVAA
                     GGDEARAKRVRDRLASKGYPAAVIQDEGGGLWRVRVGRWKDRGPAEAMAERIRKETGL
                     QPWVLREGAR"
     gene            2076..3710
                     /locus_tag="VFD06_05280"
     CDS             2076..3710
                     /locus_tag="VFD06_05280"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02980.1"
                     /translation="MRRQDEPRLLGELLLEAGVVSGAGLRHGLEAQRLGGGRLGWHLM
                     RLGEVVPVGFHLFLDTHLEGMRPDLMNDLREGSAAPFVPARLAHHYGMMPLRDAGGAL
                     EIALGSADQRGLVPAVEALTSRRVEPIICPPSFVAEAIARHYPAEVEPGVVFPAAGEA
                     ELVLRDARRGLVPAEYEALSPAAPPAEWLRALIAAALHRGARLLSLEPGRISDRMRLE
                     SGEGEEAIDLPRGPLAGVAALVDGLSRAAARGRILPREGRFTVRDEGRRIGVSALSLP
                     GLHGRSTVLHFREERILEPTPLQHALALPALGKAVERLAAERRGLLFLAATGPSEWNS
                     ALLAVLGLLGDRLPHRDVRGAWDDGAPSSLETGADLLVVASPWEAAQAAALERQADKC
                     VVLATVVAGNAFRAAQSMAQGRVGRGPSGSSAAGILAARHVEAVCGGCRRSFDFGDLL
                     DLVPEAGAARSGSFATAPGCGACRGSGRIDLVRALEYLPLGPDALDRPGRHAARLRAE
                     QAEANRPTLLDAVLSEAADGRVDVREVLRLLVHEPR"
     gene            3719..5272
                     /gene="lnt"
                     /locus_tag="VFD06_05285"
     CDS             3719..5272
                     /gene="lnt"
                     /locus_tag="VFD06_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZN02981.1"
                     /translation="MSLRALASALTSLLLVLSFPHADLGWLAFVALVPLLLALRGSAP
                     REGARLGFLAGVVFFGGLLAWLTGVMTRYGGLSVVAGAAILALLVAYLSVYVALFAAI
                     VAAAVERAGPLAYLLAPFVWTGLELLRGRLLTGFPWGTLGATQWRHPAMLQAGALGGV
                     ALVSWLVVLSNAAVAILLMRGAARRHRIVAAAALALVGIAGAAGSRAVTRLPAGEGEA
                     IPVAAVQANVPQDRKWRPEEQAAIVTHLIELSRQAVAQGARLVVWPESSSPLSYFRPA
                     GDAARAMPIEADRKDAERVAAFVRESGVTLVAGAVQYRHDGSRMRAYNSAFVSTPEGE
                     AGESYDKVHLVPFGEYVPLQRVLFFVNRMVQGAVAEFEAGDRLAPLETPFGPAGPLIC
                     YEAIFPEQVRRLASARFLLNLTNDAWFGRTAAPRQHLALATVRAAENHRWMLRAANTG
                     ISAIVDPAGRVVAETPLEEERVLVGTIRARTDMTPYAARGDLFGWGCVIVAAVAGLRW
                     RAWSARRNG"
     gene            5312..6443
                     /gene="prfB"
                     /locus_tag="VFD06_05290"
                     /pseudo
     CDS             5312..6443
                     /gene="prfB"
                     /locus_tag="VFD06_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110174822.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            6562..>7220
                     /locus_tag="VFD06_05295"
     CDS             6562..>7220
                     /locus_tag="VFD06_05295"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HZN02982.1"
                     /translation="MRSVSLGALWIPLAAAVMVMGASVPEAAVCTPGAPEICNGLDDD
                     CDGIADEDPACSTACWPGLENPLVPAPGGAPSYAPSLAWDGAGYGWVGGTFTFDRRSA
                     DGQPLGAPVSLPLDVFTPASDFRGASIVWTGTEYGVAWGDTQIHFLRLDASGQPIGPL
                     TTLPNPLAWVFFSADLAWTGTEYGLVWAETPSTFMFPDERILFARFDAPGTLLGAPRV
                     L"
BASE COUNT          959 a         2581 c         2636 g         1044 t
ORIGIN      
        1 atcgaatcgc ggcgggccgt ggtcgaggtg gagcagtacc actacgccac ctcccagctg
       61 gcgcagacga ccctgcgatc ggttctcggg caggtggagc tggacgatct cctgagccag
      121 cgggagcggc tcaacgccga cctgcagcag atcctcgacc gccacaccga cccatggggg
      181 atcaaggtct ccatggtcga ggtgaagcac gtggacctgc cgcccgagat gcagcgggcg
      241 atggcgaagc aggcggaggc ggagcgggag aagcgggcca agatcatcca cgccgacggg
      301 gagctgcagg cgtccgcatc gctcgccaag gcggcggaca tcatctccgc gaacccgacg
      361 acgctgcagc tgcgctatct ccagaccctg accgagatcg ccgccgagaa gaactccacc
      421 atcatcttcc cgatgccgat cgagctgttc cagggtctga agcacgccct cggtcccgcg
      481 cgggaccgcg cgtgagcgaa gcgccggcgc cgctcgccga ccagctggac gtcgcgcagc
      541 gggccctcga ggtctgccgc tgcatcgagc cgcagccggc cgccgaggcg ttcctgcggg
      601 gcgccgtctc ctacacgcgg cttccggtgg gagcccttta tctttatgac gccgagagcg
      661 acatgttccg gctggcggtc tccacccttc cggacggggc ggcgcagcgc gaggagatcg
      721 cctcgctcga cctcgcggcg ctgcgccggt ccgggccgcc ggagacctgg ggcccggcgg
      781 tcgtctcgcc gccgctgccg ctcgacctgc tggggagcga tccgatcgtg gcggtgccgc
      841 tcaccggcag cgggcgcctg ctgggcatgc tcgtgctggg agacgtcgcg ggcggcgccg
      901 ccgccccgga gccgctggcg cggctgcggc gtctcctgcc ggagatgctg ccggggctgc
      961 tcaacgcgca ccgggtgcag gcctaccgcg atctcgtcat caaggacgac cagagcgact
     1021 gctacaaccg gcgcttcttc gatcgctgcc tgtcggagga ggtctaccgg gcgcagcgct
     1081 acggccaggc gctgtcgctg gtcttcctcg atctcgacaa cctcaaacag gtgaacgcgc
     1141 gctatggcca cgccgcaggc tcccggaccg tgcgcgaggt gtcgcggcgg ctcctcggga
     1201 cgatccgcgg cagcgaccgc gccttccgct acggcgggga cgagttctgt ctggtgctgc
     1261 cggcgaccga cctcacggga gcgcgcgagc tgtgcgagcg cctgcgcgtc gccatcggag
     1321 ggcgtccctt cgtcgtcgac accgccgcgc acctcacggt gaccgcctcc ttcgggatcg
     1381 ccacctatcc gttgcacgcc cgcaccagcc tcggcctcat caagtgcgcc gacgaggcga
     1441 tgcagaccgc caaggcggcc ggcaagaacc tcgtctatgt cgccggcgag accgccccct
     1501 cgcccagccc gcgggcgaag gcggcggcgg agcgtcggtg agctccaccc gacgcgccgg
     1561 ccggccgatc gtggcggtgc tcgtgaccag cgtcctgatg ctcgcgggag cccgcttcat
     1621 cggccgcgtc gccgcgtcgc gcgcccccgc ggccggcgcc ggtggggacg cggcgacgca
     1681 gcaagcggcc gccgttccgg acctcacctt ctacagcacg ctcggcgacg cctctccgcc
     1741 gcgcggcaag ggaggggcga agggaacggg ggaggcggcg ctgcgggcgg cgccggggga
     1801 catcgtgccg gcggagggga tctacgtggt gcaggtggcg gcggggggtg acgaagcgcg
     1861 ggcgaaacgg gtgcgcgacc ggctcgcgtc gaaaggatat cccgcggcgg tgatccagga
     1921 cgagggcggc ggcttgtggc gcgtgcgcgt cggccgctgg aaggatcggg gcccggccga
     1981 ggcgatggcg gagcggatcc gcaaggagac cggcctgcag ccgtgggtgc tgcgcgaagg
     2041 ggcgcgatga gcgccccgtt caagaaggcc gaacgatgag gcgccaggac gagccgcgcc
     2101 tcctcggaga gctgctcctc gaggcgggcg tcgtcagcgg cgccgggctg cgccatgggc
     2161 tcgaggcgca gcggctcgga gggggccgcc tcggctggca cctgatgcgg ctcggggagg
     2221 tggtgccggt cgggttccac ctcttcctcg acacccatct cgaagggatg cgtccggatt
     2281 tgatgaacga cctgcgcgaa ggaagcgccg ctccgttcgt tcccgcgcgg ctggcgcacc
     2341 actacggaat gatgccgctg cgtgacgcag gaggcgcgct cgagatcgcc ctcggcagcg
     2401 ccgatcaacg ggggctcgtc ccggcggtcg aggcactgac ctcccgacgg gtcgagccga
     2461 tcatctgccc gccgtccttc gtcgccgagg cgatcgcccg ccactatccg gccgaagtgg
     2521 agccgggggt cgtcttcccg gccgctggtg aggcggagct ggtgctgcgc gacgcgcgtc
     2581 gcggtctggt tccggccgaa tacgaggcgc tgtcgccggc cgccccgccc gccgaatggc
     2641 tgcgcgccct gatcgcggcg gccctgcacc gcggtgcccg gctcctctcg ctcgagcccg
     2701 ggcggatcag cgatcgcatg cgcctcgaat ccggggaggg ggaggaggcg atcgacctcc
     2761 cgcgcggtcc gttggcgggc gtcgcggcgc tcgtggacgg gctgtcgcgt gccgccgcgc
     2821 gcggacggat cctgccgcgc gaggggcgct tcacggtgcg cgacgagggg cggcggatcg
     2881 gcgtgtcggc gctgtcgctt cccgggctgc acggccgctc gactgtcctg cacttccgcg
     2941 aggagcggat cctcgagccg acgccgctgc agcacgcgct cgccctcccg gcgctcggga
     3001 aggcggtcga gcgcctggcg gcggagcggc gtggtctcct gttcctcgcc gccaccggcc
     3061 cctcggagtg gaactccgcc ctcctcgcgg tcctggggct cctcggggat cgcctgccgc
     3121 accgcgacgt ccgcggcgcg tgggatgacg gagccccctc ctcgctcgag accggggcgg
     3181 atctgctcgt cgtcgcctcc ccctgggagg cggcgcaggc cgccgccctg gagcgacagg
     3241 cggacaagtg cgtcgtcctc gcgaccgtcg tggcggggaa cgccttccgg gccgcgcagt
     3301 cgatggcgca gggacgcgtc ggacgggggc cgtccggatc gtccgcggcc gggatcctgg
     3361 cggcgcggca cgtcgaggca gtctgcggcg gctgccggcg ttcattcgat ttcggcgacc
     3421 tcctcgatct cgttccggag gcgggggcgg cacgctccgg ctccttcgcg accgccccgg
     3481 gctgcggcgc ctgccgcgga tcggggcgga tcgacctcgt gcgcgccctc gagtacctgc
     3541 cgctcggacc ggacgccctc gatcgtcccg gacggcacgc ggcgaggctg cgcgccgagc
     3601 aggcggaggc gaaccggccc acgctcctcg acgccgtcct cagcgaagca gcggacggac
     3661 gggtcgacgt gcgcgaggtg ctgcgcctgc tcgtgcacga gccgcgctga tccggaggat
     3721 gagtctccgg gcgctcgcgt ccgctctcac ctccctcctg ctcgtcctgt cgtttccgca
     3781 cgccgacctc gggtggctgg cattcgtcgc gctcgtgccg ctcctcctgg ccctgcgcgg
     3841 ctcggcgccg cgcgaggggg cgcggctcgg cttcctcgcc ggcgtcgtgt tcttcggggg
     3901 gctcctcgcc tggctgaccg gcgtcatgac ccgctacggc gggctgtcgg tggtcgccgg
     3961 cgccgcgatc ctcgcgctgc tcgtcgcgta cctgtcggtg tacgtggcgc tgttcgcggc
     4021 gattgtcgcg gcggcggtgg aacgggccgg ccccctcgcg tatctcctgg cgccgttcgt
     4081 ctggaccggc ctcgagcttc tgcgcgggcg gctcctgacc ggattcccct gggggacgct
     4141 gggggcgacg cagtggcggc acccggcgat gctgcaggcg ggggcgctcg ggggggtggc
     4201 gctggtgtcg tggctcgtcg tgctctcgaa cgcggcggtc gccatcctcc tcatgcgggg
     4261 agcggcccgg cgccaccgga tcgtcgccgc ggcggcgctc gcgctcgtcg gcatcgcggg
     4321 ggccgccggc tcgcgggcgg tgacgcgtct gccggcgggg gagggtgaag ccatcccggt
     4381 ggcggcggtg caggcgaacg tccctcagga tcgcaagtgg cggcccgagg aacaggcagc
     4441 gatcgtgacg catctgatcg aactgagccg gcaggcggtc gcgcaagggg cgcgtctcgt
     4501 cgtctggccg gagtcgtcga gcccgctgtc gtacttccgg ccggccggcg acgccgcgag
     4561 agcgatgccg atcgaggcgg atcggaagga cgccgagcgg gtcgccgcct tcgtgcgcga
     4621 gagcggcgtg acgctcgtcg ccggggcggt gcagtaccgc cacgacggct ctcgcatgcg
     4681 cgcctacaac agcgccttcg tctccacgcc cgagggagag gcgggtgagt cgtacgacaa
     4741 ggtccacctc gtgccgttcg gcgagtacgt cccgctccag cgggtgctct tcttcgtcaa
     4801 ccgcatggtg cagggggcgg tggcggagtt cgaggcgggg gatcggctgg cgccgctcga
     4861 gaccccattc ggcccggccg gcccgctcat ctgctacgag gcgatcttcc cggagcaggt
     4921 gaggcgcctg gcgtcggccc ggttcctgct caatctgacc aacgacgcct ggttcggccg
     4981 gaccgcggcg ccgcgccagc acctggcgct cgccacggtg cgggccgcgg agaaccaccg
     5041 ctggatgctc cgcgccgcca acaccggcat ctcggcgatc gtcgatccgg ccggccgggt
     5101 cgtcgcggaa acaccgctcg aggaggagcg ggtgctcgtc ggaacgatcc gcgcgcgcac
     5161 cgacatgacg ccgtacgcgg cgcgcggcga cctgttcgga tggggctgtg ttatagtcgc
     5221 cgcggtcgct ggcctccggt ggcgcgcgtg gagcgcccgc cggaacggat gaaacaacgg
     5281 cggcgaccgc ctcccccgga ccttcttgct gatgacgcga tccttcgacg tcgacgaact
     5341 ggcccggcgt gtggatgcgc tgcgggcgcg ccacgccgag ctccgggtgc acctttgagc
     5401 gggccgcgcc cgaagcgcgg ctcgacgagc tcgagacccg cgccgccgat cccgatctct
     5461 tcaaggaccc gcagcgcgcc cagcaggtcc tgagggatcg cgcccgcctc accgaggcga
     5521 tcgaggcgaa gaaatcgctc gccgccaggg cggacgacgc gcagaccctc gtcgagctcg
     5581 tgcgcgaggg ggagccggcg ggggaggacc tgcaggccgc cgtcgacgcc ctcgccgcgg
     5641 ccctcgacgc gaccgagctc gagctgatgc tgtccggcga gcacgacgcg aacaacgcca
     5701 tcctcaccat ccacccggga gccggaggga ccgagtcgca ggactgggcc gagatgctgt
     5761 accgcatgta cctgcgctgg gcggaggcgc acggataccg catcgaagcg ctcgactacc
     5821 agaaggggga cgaggcgggg atcaagagcg ccaccctgat gatccggggc gccaacgcct
     5881 acggccggct gcgcgccgag aacggcgtgc accggctggt gcgcatctcc ccgttcgact
     5941 cctcggggcg ccgccatacc tcgttcgcca gcgtctacgc catcccggag ctcgacgacg
     6001 cgatcgacat caagatcgag gagaaggacc tgcgcgtcga cacctaccgc agcagcggcg
     6061 ccggcgggca gcacgtcaac gtcaccgact cggcggtccg cgtcacccac ctgccgaccg
     6121 gcatcgtcgt gtcgtgccag aacgagcgct cgcagcaccg caaccgcgag atggcgatgc
     6181 gcgtcctcag ggggcgcctc tacgaaatgg aacagcggaa gcgggacgag aagcgcaagg
     6241 cggacgaagg ggacaagaag gacatcgact tcggcagcca gatccgctcc tacgtcctgc
     6301 agccgtacca gctcatcaag gaccaccgca ccggcttcga gcgcggcgac gtggaccgcg
     6361 tgctggacgg cgatctcgac ccgttcatcc gggcgttcct gctcaagaga gtcgccccct
     6421 caggcgcgcc cgtgcagaac tgaccgttcg atccgtcttt cttgttgacg gcccccccga
     6481 acttgcgtaa atagagcgta tctcgccccc ggagcgtttc gcccgggaca tgtcacatcg
     6541 ccgtaaaggg aggtgacact gttgcgctcc gtttcactcg gcgccctgtg gatccctctc
     6601 gctgccgccg tgatggtcat gggcgcttcg gtccccgaag ccgccgtctg cactcccgga
     6661 gcccccgaga tctgcaatgg gctcgatgac gattgcgacg gtatcgccga tgaggacccg
     6721 gcgtgctcca cggcctgctg gcccggactc gaaaaccccc tggtgccggc gcccggcggg
     6781 gcgccctcct acgcaccatc gctggcctgg gacggggcag gatacggctg ggtgggtgga
     6841 acattcacct tcgaccgccg gagcgccgac ggtcagccgc tgggcgcccc ggtttccctc
     6901 cccctggacg tattcactcc cgcatcggac ttccgggggg cgtcgattgt ctggaccgga
     6961 accgaatacg gcgtcgcatg gggggacacg cagatccact ttctgcgact cgacgcctcg
     7021 ggtcagccga tcggccctct gacgaccctc cccaaccccc tcgcctgggt gtttttttcg
     7081 gccgatctcg cgtggaccgg aacggaatac ggcctcgtct gggctgaaac gccaagtact
     7141 ttcatgttcc ccgacgagcg catcctgttc gcccgtttcg acgcgccggg gacgctcctg
     7201 ggcgcgccgc gcgtcctgaa
//
LOCUS       DASRIQ010000213         7413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1904709, whole genome shotgun sequence.
ACCESSION   DASRIQ010000213 DASRIQ010000000
VERSION     DASRIQ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904709"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFD06_05300"
     CDS             complement(<1..395)
                     /locus_tag="VFD06_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN02983.1"
                     /translation="MTPAEMCRRLERLCRLASEVAALREEQSTLRHVVETAVKVVGVP
                     AAHVALVDRSRKALYGLVSSGAHPPRAPRARFVLSRGQAALMALEKRRPIVVRDARRD
                     RRVHAEARARLRLGSVAYLPLLAGKESFGL"
     gene            812..2998
                     /locus_tag="VFD06_05305"
     CDS             812..2998
                     /locus_tag="VFD06_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZN02984.1"
                     /translation="MSIVPSFLMSAAGPAAAGTSELTLARVARFPPPGARVATQFRFT
                     HDGRFLYFLSLEGPGPDRSLVRLDVRGGAREVVGRAEGTGAATLTPEEILRRERQRIQ
                     DLGITEYTLAEKADAVVYVSGGALFLVRPGSPPVALPEAGQGMLTPQLSPDGTRLAFV
                     RDGELLVMDLATRAVVTLTRGAGETLKHAVAEYIAQEEMDRPLGFWWSPDGGALAWTE
                     VDESPVPVYPIVHQGRDAWDVERPRYPFAGGPNARVRLGIVPSRGGETRWLPLAQEGE
                     DVYLARVRFGPDGALYAQVQSRDQKSLRLVRFRPPSWQATPLLEERSETWVALHEDLR
                     PLKDGRFLWSSERSGWRHLELRATDGRPVRTLTSGAWPVDRIESVDEERGIVFFTAAR
                     ESPLHKPVYRVPLEGGKVARVTPEDGFHATVFAPDGRRFVDVHDRLSQPPKALLREAS
                     GAIERVIDANDDPEVKALGLAPPELVTLQGADGTPLHGAIYHPPAGTGGPRHPAIVRV
                     YGGPTSQTVKDSWELTQDLRAQFLARRGYVVFRLDNRGTPRRGVAFERTLARRLGAVE
                     VEDQAAGARWLASRPDVDGARIGIYGWSYGGYMALLCLLRAPDIFRAGVAGAPVTDWA
                     GYDTHYTERYMGTPQENADGYRASSVLTEAARLDRPLLLVHGMSDENVHFRHTARLLN
                     VLNPAHRRYDLLLFPDERHLPRGQNDREELEARLLQHFDRHLAPGS"
     gene            complement(3065..3274)
                     /locus_tag="VFD06_05310"
     CDS             complement(3065..3274)
                     /locus_tag="VFD06_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZN02985.1"
                     /translation="MAMPRGKVKWFNDAKGYGFITPEDGSKDVFVHHTAIQSQGFRTL
                     QEGETVEFEITQGQKGPAAANVRKV"
     gene            complement(3400..4815)
                     /locus_tag="VFD06_05315"
     CDS             complement(3400..4815)
                     /locus_tag="VFD06_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN02986.1"
                     /translation="MQAAAAGRAIRRATTFQLVFLTYSVVCSGAYGLEQMVSAAGPGL
                     ALTLLLVLPVLWGIPISLACAELSSHYPVEGGYYRWVRAAFGDFIGYQAGWLVWLANL
                     MTNAAFAVLFASYLRYFIPDLGTTGHYLVALMLIWGTTLLNCFGIRLVGTTAVVLTCL
                     IFLPFLGLTAGGFLQWTHAPFVPFAHPDKTLAAAFTEGLLIALWLYGGYEKLTPNAEE
                     VENPSRAFPIALAIAMPMVIGSYLLPTFAGLVACDGWRDWGEAHFTVLAGQVGGAPLA
                     AAMSIGGLLSNTCILMVTILGQSRLPMVLAQDRLFPRLFARVDLKFGTPTPSLVAGAL
                     ALSLLAFLPFAALAGLFSLVQVLAYVLICAALLRLRRQRARGQAPAGFGSAAGAAGRP
                     FRVPLGLPGMVAILLPVFALTTFVVVRQLWPDEGLDLKAIATDLLVFASGPLTYVLFR
                     WHARRGDPEGNLGGRESSLGV"
     gene            complement(4864..6753)
                     /locus_tag="VFD06_05320"
     CDS             complement(4864..6753)
                     /locus_tag="VFD06_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02987.1"
                     /translation="MNLPCVDDGNACTDEVCPQGSCTHPPKPDGTPCADRCHTCQGGV
                     CSDTPVNCDDDNPCTDDPCDPTFGCLHTPRNCLDTNPCTFDICDASDGCRHLPAPAGD
                     QCDDGIACTIQDSCVANSGGGMVCAGTVPGCDDGNPCTTDAGVVEGDSCSCRHTPVDD
                     PCCSEGTPVSCDDGNACTVDACDGTNGDCRHDLLVCDDANACTHDVCEPQTGCVSRAD
                     PRDGQACDDLNACTRNDVCADGACGGTVLGDGEACDDSNVCTAEDQCYSGRCVGYGLE
                     GGMPCDDGNSCTADDQCLYPENGDPECHGAARAIGASCDDGDLCTVNDRCFAAGAEGG
                     APACQGTGRDCADDDACTQDACDSSTGLCAHPPRSCEDGNSCTLDSCSTQTGECVHEP
                     QDGTPCSEAGDRCHVGSCQGGACTGIAPVSCDDGNQCTFDFCDPASGCVHGGSGTFNP
                     PPEICNGVDDDCDGKVDERELAPACEVRPRTMRDGRGQDSFTVTCRWNPACAGSPVPA
                     PLDDIRPGWLSAADGLEEAGDDVVLPNPIAHCDTAIVEDDGKRTTSNAAVTFVFDPDG
                     NGVCGTTGGGRAGLMQHLADVPDGELARICVRWRLAGPERCGVVLVRHETFTEQRRER
                     RSKGQ"
BASE COUNT         1112 a         2631 c         2576 g         1094 t
ORIGIN      
        1 agtccgaagc tctccttccc ggcgaggagc ggcaggtacg cgacgctccc gagccggagc
       61 ctcgcgcgcg cctcggcgtg cacgcgccgg tcgcggcgcg cgtcgcgcac gacgatcggc
      121 cggcgtttct cgagcgccat gagcgccgcc tgcccgcggg agagcacgaa acgggcgcgc
      181 ggggcccgcg gagggtgcgc gccggagctg accaggccgt agagggcctt gcggctgcga
      241 tcgacgagcg ccacgtgcgc cgccggcacc ccgaccacct tcaccgcggt ctcgaccacg
      301 tgccggaggg tggactgctc ctcgcgcagc gccgccacct ccgacgcgag gcggcagagc
      361 cgctcgagac gccggcacat ctccgccgga gtcaaggcaa cccctcgtga gacccgacgt
      421 ttcctcgtcc tggatgcagc cattcagccc ttccccgcgg aacgccgggt gcgggcggcg
      481 atgccggacc ttctaccgct cccccggagg actgtcaact ggaattgtcg tctggatcgc
      541 cgaatccggg gtccggaccg ggggccctcc cctcttgacg attccgactt ttgaccgtac
      601 tgcggactgg gcggcgcttt cgagcgctcc cgcatcctgc gggacggccc cgctgaaccc
      661 gctcccggcc ggctccctcc accggggcgg ttccccttcc ggcgcgcatc ggcggtgagc
      721 cggaagggtg gggtcgtccc gctttcgacg cggttgacac ccgttcggag gtcggtttat
      781 accggcgcgc gtgcgccgga gcctccttct catgtcgatc gtcccgtcct tcctgatgtc
      841 cgccgcgggg cccgcggcag cggggacctc cgagctcacc ctcgcgcggg tcgcccgctt
      901 tcctcctccg ggggcccgcg tcgcgaccca gttccgcttc acccatgacg gacgcttcct
      961 ctatttcctg tcgctcgagg gcccggggcc ggaccgatcg ctcgtccgcc tcgacgtgcg
     1021 cggcggcgcc cgcgaggtcg tggggcgcgc cgaggggacc ggcgcggcca cgctgacgcc
     1081 cgaggagatc ctccgccgcg agcgacagcg catccaggat ctcgggatca ccgagtacac
     1141 gctcgccgag aaggcggatg cggtggtgta cgtctcggga ggcgcgctgt tcctcgtccg
     1201 tcccgggtct ccgccggtgg cgcttcccga ggcgggccag gggatgctga cgccacagct
     1261 ctcgcccgac gggacacgcc tcgccttcgt gcgcgacggc gagcttctgg tgatggatct
     1321 cgccacgcgc gcggtcgtca ctctgacacg aggcgcgggc gagaccctga agcacgcggt
     1381 cgcggagtac atcgcccagg aggagatgga ccggccgctc gggttctggt ggtcccccga
     1441 cggcggcgcc ctcgcctgga ccgaggtgga cgaatcgccg gtgccggtct atccgatcgt
     1501 ccaccagggg cgcgacgcgt gggacgtcga gcgcccccgc tacccgttcg ccggcggacc
     1561 gaatgcccgg gtgcggctcg ggatcgtgcc gtctcgcggc ggagagacgc gctggctgcc
     1621 gctggcgcag gagggcgagg acgtttacct ggcgcgcgtg cgcttcggcc cggacggcgc
     1681 cctgtacgcg caggtgcaga gccgcgatca gaaaagcctg cggctggtgc ggttccgccc
     1741 gccgtcgtgg caggcgacgc cgctgctcga agagcgctcc gagacctggg tggcgcttca
     1801 cgaggatctc cgtccgctca aggatgggcg tttcctctgg tcgagcgagc gctccggctg
     1861 gcggcacctg gagctgcgcg ccactgacgg ccgccccgtg cgcacgctca cctccggagc
     1921 ctggccggtc gatcggatcg agagcgtgga cgaggagcgc ggcatcgtct tcttcacggc
     1981 ggcgcgcgag tcgcccctgc acaagccggt ctaccgcgtg ccgctcgaag gcggaaaggt
     2041 cgcccgcgtc acccccgagg acggattcca cgcgacggtg ttcgctcccg acggccggcg
     2101 gttcgtggac gtgcacgacc ggttgtcgca gcccccgaag gctctcctgc gcgaggcgtc
     2161 cggcgcgatc gagcgggtga tcgacgcgaa cgacgacccg gaggtcaaag cactcgggct
     2221 cgcgcctccc gagctggtca cgctgcaggg ggcggacggc acgccgcttc acggcgccat
     2281 ctatcatcct cccgcaggga cgggcggccc gcggcatccg gcgatcgtgc gggtctacgg
     2341 cggaccgacg tcgcagacgg tgaaggactc gtgggagctg acgcaggatc tccgggcgca
     2401 gttcctggcg cggcgcggct acgtcgtgtt ccggctcgac aaccgcggca cgccgcgccg
     2461 cggcgtcgcc ttcgagcgca cgctcgcccg ccggctcggc gcggtcgagg tggaggacca
     2521 ggcggccggc gcgcgctggc tcgcgtcacg cccggatgtc gacggcgcgc gcatcgggat
     2581 ctatggctgg tcgtacggcg ggtacatggc gctcctgtgc ctgctgcgcg ccccggacat
     2641 cttccgggcc ggggtcgcgg gcgcgccggt gaccgactgg gccggatacg acacccatta
     2701 caccgagcgc tacatgggaa cgccgcagga gaacgcggat ggatatcgcg cttcgtccgt
     2761 cctcaccgag gcggcgcggc tcgaccgccc gcttctgctc gtccacggga tgtcggacga
     2821 gaacgtgcac ttccggcaca cggcgcggct gctcaacgtg ctgaacccgg cgcaccgtcg
     2881 ctacgacctg ctcctcttcc ccgacgagcg tcacttgccg cgcggccaga acgatcgcga
     2941 ggagctggag gcgcgcctcc tccagcattt cgatcgccac ctcgctccgg ggtcctgaag
     3001 cacgaagggc cgctcccctt acgggaagcg gccctgtatg gccaagaatc gtgcggtgtg
     3061 tccgctagac tttgcggacg ttcgcggcgg cggggccctt ctgcccctgc gtgatctcga
     3121 actccaccgt ctcgccttcc tggagggtgc ggaatccctg ggactggatt gccgtgtgat
     3181 gcacgaagac gtccttgcta ccgtcctcgg gcgtgatgaa accgtacccc ttcgcgtcgt
     3241 tgaaccactt caccttcccc ctgggcatag ccactccacc tccggaaagc acgggactcc
     3301 cctgacggcg caacgccacc gctggcgccg catacccatt atcctgtcga gggctgtccc
     3361 atggaacgcg tcattctcct cgatgtatcg ttgcggtgtc tagaccccca aggaagattc
     3421 ccggcccccc aggttcccct ccggatcccc cctcctggca tgccatcgga agaggacgta
     3481 cgtcagggga cctgaggcga aaaccaggag atcggtcgcg atcgccttga ggtcgagccc
     3541 ctcgtcgggc cagagctggc gcacgacgac gaacgtggtc agggcgaaca ccggcagcag
     3601 gatcgcgacc attcccggca ggcccagcgg cacgcggaac ggccgacctg cggcgccggc
     3661 ggcggacccg aaaccggccg gggcctggcc gcgcgcgcgc tgtcgtcgca gacgcaggag
     3721 cgcggcgcag atgagcacat aggccagcac ctgcacgagc gagaagagcc ccgcgagcgc
     3781 cgcgaacggc aggaacgcca gcaggctcag ggccagggcg ccggccacga gcgacggcgt
     3841 cggcgttccg aatttcaggt ccacgcgcgc gaagagacgc gggaagagcc ggtcctgagc
     3901 cagcaccatc ggcagcctcg actggccgag gatcgtcacc atcaggatgc atgtgttgct
     3961 caggagcccg ccgatcgaca tggcggctgc gagcggcgcc ccgcccacct gacccgccag
     4021 gacggtgaag tgcgcctctc cccagtcccg ccagccgtcg caagcgacca gcccggcaaa
     4081 ggtggggaga aggtagctgc cgatcaccat cggcatcgcg atcgcgagcg cgatcgggaa
     4141 ggcgcgcgat ggattctcaa cctcctcggc gttgggagtg agcttctcgt acccgccgta
     4201 gagccacagg gcgatcagaa gaccttccgt gaaggcggca gcgagcgtct tgtcgggatg
     4261 ggcgaagggg acgaaggggg cgtgagtcca ttgcaggaat ccgccggccg tcagccccag
     4321 gaacggcagg aagatgaggc aggtgaggac gaccgccgtc gtcccgacca gccggatgcc
     4381 gaagcagttc aggagtgtcg tcccccagat gagcatgagg gccacgaggt aatggccggt
     4441 cgtcccgaga tccgggatga agtaacgcag gtagctcgcg aacagcaccg cgaaggcggc
     4501 gttcgtcatg aggttcgcga gccacacgag ccagcccgcc tggtacccga tgaaatcccc
     4561 gaaggcggcc cgcacccagc ggtagtaccc cccttccacc gggtagtgcg acgacagctc
     4621 ggcacacgcg agggagatgg ggatgcccca cagcaccggc agcacgagga ggagcgtcag
     4681 cgcgaggccg ggaccggccg ccgagaccat ctgctcgagg ccgtacgccc cgctgcagac
     4741 gacggaatag gtcaggaaga cgagctggaa ggtggtggcg cgccggatcg cgcgtcccgc
     4801 ggccgcggcc tgcatcgccg cggactctag gcgctcgcgt cgccggccgt caaggcgagg
     4861 cgctcattgc cccttgctcc ggcgctcccg acgttgctcg gtgaaggtct cgtgcctgac
     4921 cagcacgaca ccgcaccgct cggggccggc gaggcgccac ctcacgcaga tcctcgccag
     4981 ctcaccgtcc gggacgtcgg ccaggtgctg catgagcccg gcccgtcccc cgccggtggt
     5041 accgcagacg ccgttgccgt ccggatcgaa cacgaacgtc acggccgcgt tgctcgtcgt
     5101 ccgcttcccg tcatcctcga cgatggcggt gtcgcagtgc gcgatcggat tcggcagcac
     5161 cacgtcgtca cccgcctcct cgagcccatc cgccgccgac agccagccgg gccggatgtc
     5221 gtcgagcggc gccggaaccg gcgatcccgc gcaggcgggg ttccaccggc aggtgacggt
     5281 aaagctgtcc tgtcccctgc cgtcccgcat ggtgcgcggc cggacctcgc acgccggcgc
     5341 gagctcccgc tcgtccacct ttccgtcgca gtcgtcgtcg acgccgttgc agatctcggg
     5401 gggaggattg aacgtgccgc tcccgccgtg gacgcatccg ctcgccggat cgcagaaatc
     5461 gaacgtgcac tgattcccgt catcgcacga gacgggcgcg atgcccgtgc aggcgccgcc
     5521 ctggcaggag ccgacgtggc accgatcgcc cgcctcgctg cagggggtgc catcttgcgg
     5581 ttcgtggacg cactcccccg tctgggtgga gcaggaatcg agcgtgcagg agttgccatc
     5641 ctcgcagctc cgaggtggat gcgcgcacag ccccgtgctg gaatcgcacg catcctgggt
     5701 gcaggcatca tcgtcggcac aatcgcggcc ggtcccctgg cacgcgggag ccccgccctc
     5761 cgcccccgcg gcgaagcagc gatcgttcac ggtacagagg tcgccgtcgt cgcaggacgc
     5821 tccgatcgcg cgggccgccc cgtggcattc aggatcgccg ttttccggat agaggcactg
     5881 gtcgtccgcc gtgcaggagt tgccgtcgtc gcacggcatg cccccctcga gcccgtatcc
     5941 cacgcagcga cccgagtagc actgatcctc ggccgtgcag acattgctgt cgtcacaggc
     6001 ctcgccgtct ccgagcaccg tgccgccaca cgcgccgtcg gcgcacacgt cgttccgagt
     6061 gcacgcattc agatcgtcgc aggcctggcc gtcccgcggg tcggcgcggg agacgcagcc
     6121 ggtctgcggc tcgcagacgt catgggtgca ggcattggcg tcgtcgcaga ccaggaggtc
     6181 gtggcggcag tcgccgttcg tgccgtcgca ggcatcgacc gtgcaggcat tgccgtcgtc
     6241 gcaggagacg ggggtcccct ccgagcagca cggatcgtcc accggcgtgt gacggcagga
     6301 gcagctgtca ccctccacga ctccggcgtc ggtcgtgcac ggattgccat cgtcgcagcc
     6361 cggaacggtc ccggcgcaga ccatgccccc tcccgagttc gcgacgcagc tgtcctggat
     6421 cgtgcaggcg atgccatcgt cgcactggtc gccggcgggc gccggcaggt gccggcaccc
     6481 gtccgacgcg tcgcagatgt cgaacgtgca ggggttcgta tcgaggcagt tccggggcgt
     6541 gtgcaggcag ccgaaggtcg ggtcgcatgg atcatccgtg cagggattgt cgtcgtcgca
     6601 gttcaccggc gtgtcactgc agacgccgcc ctggcaggtg tggcagcgat cggcacacgg
     6661 cgtcccgtcg ggcttgggag gatgggtgca ggagccctgc ggacagacct cgtcggtaca
     6721 ggcgttgccg tcgtcgacgc agggcagatt caatgccggc tgacaacctc ctcgaatgca
     6781 gtgagcgagc ccgcagtagc cgggaacaac acaagaggtg aacggcgact gatctcggga
     6841 ctgacaggct ccgtgctcgc aaacatagaa atagcattgg cccggaggag gacacggccc
     6901 gatatcgtcc aatcggcaga agtcgtcgtc ctcatcgcag cgtccgaccc cgcatcctcc
     6961 gctggggcag acgcgggcct gtccgttctg gcagttcccg ccggagcaga cgtcattcac
     7021 ggtgcagaac cggccgtccg agcagggacc gtcggcatgg tcccgccggc acagcccggt
     7081 cgcgggatcg cagacatcgg tcgtgcagac attcccgtcc tgacacgaga ccgggggatg
     7141 gagacactcc ccggtcgcgg cgtcgcaacg atcgtctgtg cagaggtctc cgtcggagca
     7201 gtcgcggctg gtcccctcgc aggccgccga gccgtcttcg cccgtcacgc agcggtcgtc
     7261 gcgcgtgcag gcattccgat cgtcgcagcc cgccccgtcg ccgagggggg ttccgctgca
     7321 ctgatgggac gaatcgcagg cgtcggagac ggtgcagggg ttgccgtcat cgcacggcgt
     7381 gttcgcgccg agaaactcgc cggggacgca gag
//
LOCUS       DASRIQ010000214         7688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_859843, whole genome shotgun sequence.
ACCESSION   DASRIQ010000214 DASRIQ010000000
VERSION     DASRIQ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859843"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFD06_05325"
     CDS             <1..711
                     /locus_tag="VFD06_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN02988.1"
                     /translation="TREARRGVMRRVVTLLKKGLFYSFSVAKTSWVSAEYFEHNINAL
                     LDDVGIDQTGIPFCAVAADLLGGGPIVLRQGRLRAAVSASCAVPGLLPPVRIDGRLLI
                     DGGWVAQVPVVPALQEGADLVIAVDVSNDLEALSDLTTGLNIMVRASGMRAEALRGMQ
                     GRLADVLIEPEVGRIHWADFGAVEECIRLGEQAAEARLGEIRQALRLARWPSFIGFSR
                     SRRLTRARLERERQKKVS"
     gene            725..2788
                     /locus_tag="VFD06_05330"
     CDS             725..2788
                     /locus_tag="VFD06_05330"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZN02989.1"
                     /translation="MSGARTLKWGLGIVAAVVVTLIAAVVLLPLLIDVNRYAPLLSAQ
                     TQSLTGRAAKFGPMTLRVLPAPALHVAPVSLAEGARYPGRDFIRIESIDVRLRLLPLF
                     RGRVEFGTIVLDRPTVTVIRDRQGRWNFDDLLERTAALRRQGAGAAGAPPTAGAGPTI
                     GVGEAVLRGGRLLVYDDAVVPGRRSELTVGPIDARLAGFGLRQESDADLSLGLGKSRL
                     AVTARLSGDGEAQVVEAKIDRSRLETADLVPLIPWLGVARPAGLQVGGGADVEGKATV
                     PLARPETLQFDGTITLDNLHYKDAGMTRAVEKVGGRLKVAGDRASWEGFTATVGKSDL
                     SGTLTVEDYLRPRVGFALTSKRLDLNELVALYAPAPAGRAAAPAAPAAGGSSEADLLS
                     QVSAAGTLKADALRFQTFDLADVKGKVTLKDGVANLSDLDARIADGTIRGSAGLDLGR
                     PKPGYRLEAALKKVDVNALATAYDPGLKDLLRGRLGGELSLAATGTDLDAILSSARGN
                     ARIEIADGAVTSISILKQLASLLEMAGGKGIGKDETPFDMLRGNFAIGDRRAATNDLS
                     LDSKDLDLAGIGHVGLDASLDLAVTGRFSEEASRGMIEKTPQVKALADSAGIVTVHLL
                     ARGPLAAPQVGLNTRAQVRQVQEQKKEQIKEKVRDRLLDYLGGGKKKEEPPTPEPTPP
                     PPPPR"
     gene            complement(2789..3262)
                     /locus_tag="VFD06_05335"
     CDS             complement(2789..3262)
                     /locus_tag="VFD06_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02990.1"
                     /translation="MRWRGMLLKWGLPALLLSMAAGSASLDAGKQPKPEDDPEERDKP
                     KLRLLAEPTYGFTPVTALLTAQLTGIAPDDPNFCHPSVTWIRIRPGQTEENATRIHED
                     PVCRHPETETAAATSFIKTFDLYQPGTYLFRIIVEGKDHHRIQSGTAQVVVLRVQ"
     gene            3473..5506
                     /locus_tag="VFD06_05340"
     CDS             3473..5506
                     /locus_tag="VFD06_05340"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HZN02991.1"
                     /translation="MFAKTGVRASGRFGRLLVAAGLLLPLAAALPPAAFAGEEDEEAG
                     SIYARVRWLDSGLSVERPSEGEVLAASANLPIIPGDRVSTGGGRAEIELADSSVLWLG
                     EGTRLEVRNLADYDTRYERTNLLVLRRGALRIDNRDSTDSESVFQIDTEAGSIYLLSG
                     GSFRIEANGSGTIVSAYSGVAEFSGEGGSVLVRTGQRSSVAIGAVPDEPRRFNTGRSD
                     DFDRFHDDRVAAYVSDGSEPPEYEEVPVEVRPYVRELSSYGTWTTIAPYGVVWRPVFA
                     GGWSPYTHGTWGWYPTGWVWISYDPWGWAPYRYGRWDYAPSYGWFWVPGRIWSGAWVS
                     FAVSTTYVGWCPLNYWNRPVFHDPWIAGVPAVSVARLDPRGWQFVPAGRFGARGVAVA
                     ASRIDHLPRGTEVVVTRSLPAPLNPRAVATRTDPARGFYDQVRRSRVALPAASSSSGE
                     PVSFRVQEHRQSVPPGAPKRAAPPRGRASQPRLSPSGAIAVPHQSAAASPAPRARTAR
                     PDDGTRTEPPKSSRAPHDTQARQQPQGRVVGQMAAPPQASRTTAPRTTTPTRTTTAPP
                     APRNADPSRATRQNPSRTPQETPPPAPHDPAVGRLVGGARPAPATPPQNSTSPTATPR
                     SATPASQAEPDPTASQNRGASRSDSAREKQHQGKRQGKGPEKPKEQDNRASGS"
     gene            5578..6105
                     /gene="def"
                     /locus_tag="VFD06_05345"
     CDS             5578..6105
                     /gene="def"
                     /locus_tag="VFD06_05345"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZN02992.1"
                     /translation="MAILPILRYPHEVLLTRTEDVTDITPDVERLVRDMIETMHAAPG
                     VGLAANQVGVGKRIAVVDLSVGEDPKQLLVLINPRILENHGGYDDEEGCLSLPGFTER
                     VRRPDRAVIEAMDLEGKRRTYEGRELLARAFNHEIDHLEGRLFIEHLSVLKRRLIKRK
                     VQKKMRAGDWQLAPS"
     gene            6116..7078
                     /gene="fmt"
                     /locus_tag="VFD06_05350"
     CDS             6116..7078
                     /gene="fmt"
                     /locus_tag="VFD06_05350"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZN02993.1"
                     /translation="MRVVFMGTPEFAVPSLDRLAADGHDIPLVVTRPDSRRNRRARPE
                     PSPVKARARALGLEVAEPQSAREPALLERIASLRPDLAVVVAYGKILPAALLEIPSRG
                     AVNVHASLLPRWRGAAPIARAILAGDAVTGVTTMQMELGLDTGPVLLERSCDIAPRET
                     TATLTGRLAVLGADLLAETLPLLARGAITPRPQDESRATWAPPLRREDGRIGWDAPAV
                     EIDRRIRACHPWPVAEAGLRGDRVQILEAEPLEDREPTDQPADACEPGWVLRAGESIV
                     VACGGGTRLAVLTLRVPGGRALSARDAVNGRLVARGECFTAPPG"
     gene            7171..>7688
                     /locus_tag="VFD06_05355"
     CDS             7171..>7688
                     /locus_tag="VFD06_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZN02994.1"
                     /translation="MNERILSLLRRSARTGIKIAVVGAVVVLVAAISAYVTVRRAVSG
                     RDIVVPEVIGMTADEATVALRKQALMLEPVSERHDAHVEAGHILAQEPAPGSTIKAER
                     KVKVVLSLGDEGAVIPELRGSAARGAQIALQQQGYRLSGQVYAYSRKEDENLVIAQDP
                     PAGGAGVKEGRVS"
BASE COUNT         1102 a         2691 c         2785 g         1110 t
ORIGIN      
        1 acccgcgagg cgcgccgcgg cgtgatgcgg cgggtcgtga ccctgctgaa gaagggcctc
       61 ttctattcct tctcggtcgc caagacctcc tgggtctcgg ccgagtactt cgagcacaac
      121 atcaacgccc tgctcgacga cgtcggcatc gaccagaccg ggattccgtt ctgcgccgtc
      181 gccgccgatc tcctcggcgg cgggccgatc gtgttgcggc aggggcggct gcgcgcggcg
      241 gtgagcgcct cgtgcgccgt tccggggctg ctgccaccgg tgcgcatcga cgggcggctg
      301 ctcatcgacg gcggttgggt ggcgcaggtt ccggtcgtgc cggcgcttca ggagggggcc
      361 gacctggtga tcgccgtgga cgtctcgaat gacctcgagg ccctctccga tctcaccacc
      421 gggctcaaca tcatggtgcg cgccagcggc atgcgcgccg aggcgctgcg cgggatgcag
      481 ggacgtctcg ccgacgtgct gatcgagcct gaggtcggcc ggatccactg ggccgatttc
      541 ggcgcggtcg aggagtgcat ccggctcggg gagcaggcgg cggaggcgcg gctgggggag
      601 atccgccagg cgctgcggct ggcgcgctgg ccctccttca tcggcttctc ccgctcgcgc
      661 cggctgacgc gcgcccgcct cgagcgggag cggcaaaaga aggtatcctg agcgcgagtt
      721 gccgatgagc ggggcgcgta ccctgaagtg gggcctgggg atcgtcgcgg cggtcgtggt
      781 gaccctgatc gcggcggtcg tcctcctgcc gctcctcatc gacgtcaacc gctacgcccc
      841 gctgctgagc gcgcagaccc agtcgctcac cggtcgagcc gcgaagttcg gcccgatgac
      901 gctgcgggtc ctgccggcgc cggcgctgca cgtcgcgccg gtgtccctcg ccgagggggc
      961 gcgttacccg gggcgcgact tcatccgaat cgagtcgatc gacgtgcggc tgcggctcct
     1021 gccgcttttc cgcggccgcg tggagttcgg gaccatcgtg ctggatcgtc cgaccgtcac
     1081 ggtgatccgc gatcggcaag gtcgctggaa cttcgacgat ctcctcgagc gcaccgccgc
     1141 actccggcgg caaggagccg gcgcggccgg ggcgccaccc accgccggcg ccggcccgac
     1201 gatcggcgtg ggcgaggcgg tgctgcgagg cggccggctc ctcgtttacg acgacgcggt
     1261 ggtaccgggc cgccgatccg agctgaccgt cggtccgatc gacgcgcgcc tcgcgggatt
     1321 cggcctccgc caggagagcg atgccgacct gtcgctcggt ctgggaaaga gccggctcgc
     1381 cgtcaccgcc cgtctgtcgg gggacgggga ggcgcaggtg gtcgaagcga agatcgaccg
     1441 ctcccgtctc gagacggccg atctcgttcc gctcattccg tggctcgggg tggcccggcc
     1501 ggccgggttg caggtggggg gtggcgcgga cgtcgagggg aaggcgaccg tgccgctggc
     1561 gcgcccggag acgctgcagt tcgacgggac gatcacgctc gacaacctcc attacaagga
     1621 cgccggcatg acgcgggcgg tggaaaaggt cggcggccgc ctcaaggtcg ccggagaccg
     1681 ggcctcgtgg gaagggttca ccgcgaccgt cgggaagtcc gatctctcgg gcacgctgac
     1741 ggtcgaggac tatctccgcc cgcgcgtcgg cttcgccctc acgtccaagc ggctggacct
     1801 gaacgagctc gtcgcgctgt acgcgccggc gccggccggg cgggccgcgg cgccggccgc
     1861 ccccgccgcg ggcggatcga gcgaggccga cctcctgtcc caggtgagcg cggccgggac
     1921 gctcaaggcg gacgccctgc gcttccagac gttcgatctt gccgacgtga agggaaaggt
     1981 gacgctcaag gacggcgtcg cgaacctctc cgatctcgac gcccggatcg ctgacgggac
     2041 gattcgcggc tcggccgggc tcgatctcgg gcggccgaag ccggggtacc gtctcgaggc
     2101 ggcgctcaag aaggtggacg tcaacgccct cgcgaccgcc tacgatccgg gcctcaagga
     2161 tctgctgcgg ggccgcctgg gcggggagct gtcgctcgca gcgacgggga cggatctcga
     2221 cgccatcctg tcgtcggccc gcgggaacgc gcgcatcgaa atcgccgacg gcgcggtcac
     2281 ctcgatcagc atcctcaagc agctggcatc gctcctcgag atggccggcg gcaaggggat
     2341 cggcaaggac gaaacgccgt tcgacatgct ccgggggaac ttcgccatcg gcgaccggcg
     2401 cgcggcgacc aacgacctga gcctcgattc gaaggatctc gatctcgcgg ggatcgggca
     2461 cgtcgggctc gacgcctcgc tcgacctggc cgtgaccggc cggttttccg aggaggcgag
     2521 ccgcggaatg atcgagaaga ccccgcaagt gaaggcgctc gccgattccg cgggaatcgt
     2581 caccgtgcac ctcctggcgc gcgggcctct cgccgcccct caggtcggac tcaacacccg
     2641 cgcacaggtg cgtcaggtgc aggagcagaa aaaggagcag atcaaggaga aggtccggga
     2701 tcgcctgctc gactacctcg gcggcgggaa gaagaaggag gagccgccga cccccgagcc
     2761 gactccgccg ccgcccccgc cgcgctgatt actggactct caggacgacg acctgcgccg
     2821 tacccgactg gatgcggtga tgatccttcc cctcgacgat gatccggaag aggtacgtcc
     2881 ccggctggta caggtcgaag gtcttgatga agctggtcgc ggcggcggtc tcggtctcgg
     2941 gatggcggca gaccggatct tcgtggatcc gggtcgcatt ctcctcggtc tggcccggtc
     3001 ggatccggat ccaggtcacc gaggggtggc agaagttcgg gtcgtcgggg gcgatgccgg
     3061 tgagctgcgc cgtcagcagg gcggtcaccg gcgtgaaacc gtaggtcggc tcggcgagaa
     3121 gtcgaagctt cggcttgtcg cgctcttccg ggtcgtcctc gggcttcggc tgctttccgg
     3181 cgtcgagcga cgcgcttccg gctgccatcg acagaagaag cgctggaagt ccccatttca
     3241 agagcattcc gcgccaccgc atgcaccgag tataggacag ccgtccgatc acgtttcgga
     3301 catcgaccgc cgtggatatc aggagcgtcc ccacggcggc gcgagcgtcc accgtcaagg
     3361 gccccgaaat ggccgcggtt caggttccgc accgtggcat agttttggct ctgcgccggt
     3421 cggccgggga aaaccagacc agagaaaaga cggcggaaag gcgggcctca ctatgttcgc
     3481 gaaaaccggg gtgcgcgctt cagggcggtt cggtcgcctg ctcgtggcgg cggggttgct
     3541 cctgccgctc gctgccgccc tccccccggc cgcgttcgcc ggggaggagg acgaggaggc
     3601 cggcagcatc tatgcgcggg tgcgctggct ggactccggg ctgagcgtcg agcgcccgtc
     3661 ggagggagaa gtcctcgccg cgtcggcgaa ccttccgatc atccccggag accgggtgtc
     3721 gaccggcggg gggcgggccg agatcgagct ggccgattcg tccgtgctct ggctcggcga
     3781 agggacgcgc ctcgaggtgc gcaacctcgc cgactacgac acccgctacg agcgcacgaa
     3841 tctcctggtg ctgcgccgcg gggcgctgcg catcgacaat cgcgacagca ccgactccga
     3901 aagcgtcttt cagatcgaca ccgaggccgg ttcgatctat ctcctgtcgg ggggctcgtt
     3961 ccgcatcgag gcgaacggct ccgggacgat cgtgtcggcg tatagcggcg tcgccgagtt
     4021 ctccggcgag ggcgggtcgg tgctcgtccg gaccggccag cgcagcagcg tcgccatcgg
     4081 ggctgtgccg gacgaaccgc gccgattcaa caccggccga tccgacgact tcgatcgttt
     4141 ccacgatgat cgcgtcgccg cctatgtgag cgacggatcg gagcctccgg agtacgagga
     4201 ggtcccggtc gaagtccggc cttacgtccg cgagctgtcg tcctacggaa cgtggaccac
     4261 gatcgccccg tacggcgtgg tctggcggcc ggtcttcgcc ggcggctggt cgccgtacac
     4321 gcacggcacg tggggctggt atccgaccgg ctgggtgtgg atctcgtacg acccgtgggg
     4381 ctgggcgccg taccgctacg gccgctggga ctacgcgccg tcctatggct ggttctgggt
     4441 cccggggcgc atctggagcg gcgcgtgggt gagcttcgcc gtgtccacca cgtacgtcgg
     4501 ctggtgcccg ctgaactact ggaaccgtcc cgtcttccac gacccctgga tcgcgggtgt
     4561 ccccgcagtg tcggtcgccc gtctcgatcc acgcggctgg cagttcgtgc cggccggacg
     4621 cttcggagca cgcggcgtcg cggtcgccgc cagccgcatc gaccacctgc ctcgcggaac
     4681 ggaggtcgtc gtcacccgct cgctccccgc ccctctgaat ccgcgcgccg tggcgacgcg
     4741 gacagatccc gcgcggggtt tctacgatca ggtgcggcgg agccgggtgg cgctgccggc
     4801 cgcgagcagc tcatcgggcg agccggtgtc gttccgggtc caggagcacc ggcagtcggt
     4861 cccacccggc gcgccgaagc gtgcggcccc gccgcgcggc cgtgcctccc agccacgcct
     4921 gtctccgtcc ggcgcaattg cggtgccgca tcagtcggcg gcggcctccc cggcgccgcg
     4981 cgccaggaca gcgcggccag atgacggcac ccgcactgaa ccgccgaaat cttcgcgcgc
     5041 gccgcacgac acgcaggcgc gccagcagcc gcagggtcgg gtcgtggggc agatggccgc
     5101 gcccccgcag gcatcccgga cgaccgcccc caggacgacg acgcccacga ggacgacgac
     5161 cgcgcctccg gcgccgcgca acgccgaccc ttcgcgcgct acccggcaga acccgtcgcg
     5221 gactccgcaa gagacccccc ctccggcacc gcacgatccg gcggtgggcc ggctcgttgg
     5281 cggcgcccgg ccggcgcctg ccacgcctcc gcagaactcg acgtcgccga cggcgacccc
     5341 gcgctccgcc acccccgcct cgcaggcgga accggatccc accgcatcgc agaaccgggg
     5401 cgcctcgcgc tccgattcgg cccgggagaa gcaacaccag gggaaacggc aggggaaggg
     5461 gcccgaaaag ccgaaggagc aggacaaccg ggcctccggc agctaggaca ggctcctaga
     5521 gtcccttcgt cgcgcgggtt tgaccccccc gggggcgtgt gatagatagg cgccccgatg
     5581 gcgatcctcc cgatcctccg atatccccat gaagtgctcc tgacgcgcac cgaggacgtc
     5641 accgacatca ctccggacgt cgagcggctg gtgcgcgaca tgatcgagac gatgcacgcc
     5701 gcgccggggg tcggtctcgc ggcgaaccag gtcggggtcg gcaagcggat cgcggtcgtc
     5761 gacctgtcgg ttggggagga cccgaagcag ctcctcgtcc tgatcaaccc gcgcatcctc
     5821 gaaaaccacg gcggctacga cgacgaggaa gggtgcctga gtctccccgg attcaccgag
     5881 cgcgtgcgtc gccccgatcg ggcggtcatc gaggcgatgg acctcgaagg gaagcgccgc
     5941 acctacgagg gtcgcgaact cctggcgcgg gccttcaacc acgagatcga tcacctcgag
     6001 ggccggttgt tcatcgagca cctcagcgtc ctgaagcgcc gccttatcaa gcgcaaggtc
     6061 cagaagaaga tgcgggcggg ggactggcag ctcgctccga gctgagtccg gcgccatgcg
     6121 cgtcgtgttc atgggaaccc cggagttcgc ggtcccgtcc ctcgatcggc tggcggcgga
     6181 tgggcacgac atcccgctgg tcgtcacccg tccggacagc cgccgcaacc gcagggcgcg
     6241 gcccgagccc tcgccggtca aggcgcgcgc ccgagcgctc ggtctcgagg tcgcggagcc
     6301 gcaaagcgcc agggagccgg cgctcctcga gcggatcgcc tcgcttcgcc ccgacctcgc
     6361 cgtcgtcgtc gcctacggga agatcctgcc ggccgcgctc ctggagatcc cgtcgcgggg
     6421 ggcggtgaac gtgcacgcgt cgctcctgcc gcgctggcgg ggggcggcac caatcgcccg
     6481 ggcgatcctc gcgggcgacg ccgtgaccgg cgtcacgacc atgcagatgg agctggggct
     6541 cgacaccggc cccgtcctcc tcgagcgctc ctgcgacatc gctccgcgcg agaccaccgc
     6601 gacgctcacg gggcgcctgg ccgtcctcgg agcggacctg ctcgctgaaa cgctgccgct
     6661 cctggcgcgc ggcgccatca cgccgcgccc tcaggatgaa tcgcgcgcca cctgggctcc
     6721 tcccctccgt cgcgaagacg ggcggatcgg gtgggacgcg ccggcggtcg agatcgaccg
     6781 gcgcatccgc gcctgccatc cgtggccggt ggcggaggcg ggcctgcgcg gggaccgggt
     6841 gcagatcctc gaggcggagc cgctcgagga cagggaaccg accgaccagc cggccgacgc
     6901 ttgcgagccg gggtgggtgc ttcgagcggg ggagagcatc gtcgtcgcgt gcggtggcgg
     6961 gacccggctc gccgtcctga cgctgcgggt gccgggcggg cgcgcccttt cggctcggga
     7021 cgccgtgaac ggacgcctcg tggcccgggg cgaatgcttc accgcccccc ctgggtgaga
     7081 tcgctgtgct accatcgccg tccctgcgcg cgtcggaagt cggccgtccg acccgaccgc
     7141 ggtgaccgcg gtccacgcgc gatgacccgg atgaacgaac gcatcctgtc gctcctgcgc
     7201 cggtccgcca ggaccgggat caagatcgcc gtcgtcggcg cggtcgtggt gctggtggcc
     7261 gccatcagcg cctacgtcac ggtgagacgc gccgtctcag ggcgcgacat tgtggtgccc
     7321 gaggtgatcg gtatgacggc cgacgaggcg acggtggcgc tgcgcaagca ggcgctgatg
     7381 ctcgagccgg tgtcggagcg gcacgacgcg cacgtcgaag cgggtcacat cctggcgcag
     7441 gagccggccc ccggttcgac gatcaaagcg gagcggaagg tgaaggtcgt cctcagcctc
     7501 ggcgacgaag gggcggtgat tccggagctg cgcggcagcg ccgcgcgcgg cgcccagatc
     7561 gccctgcagc agcagggata ccgcctgtcg gggcaggtgt atgcgtactc ccgcaaggag
     7621 gacgagaacc tggtgattgc ccaggatcct ccggctggcg gtgccggggt gaaggaggga
     7681 cgcgtctc
//
LOCUS       DASRIQ010000215         5299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2420168, whole genome shotgun sequence.
ACCESSION   DASRIQ010000215 DASRIQ010000000
VERSION     DASRIQ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420168"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFD06_05360"
     CDS             complement(<1..263)
                     /locus_tag="VFD06_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02995.1"
                     /translation="MANAPRPAKAPPRRRRLDRADDSTDRLERLVKRLLAGWRSRRGR
                     DPIVPSPAQVRSLAPRFSRPLPWRPEPFSRIAADLESLLALSR"
     gene            332..2332
                     /locus_tag="VFD06_05365"
     CDS             332..2332
                     /locus_tag="VFD06_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZN02996.1"
                     /translation="MLRLSGVTRAYGVHEVLKGVDWGVPPRARVGLIGPNGSGKTTLL
                     RVIAGREEPDKGTITLTSGLTLGYLAQEGARLAEGTVLDALLAPFEKVAALERELEAL
                     HHEMATAQGERMDTLIRRAGDVQHRFEAAGGFTLETQAKTILGGLGFAPDDHARPLKE
                     FSGGFRMRAALGALLMQQPDYLLLDEPTNHLDLEAVQWLEQFLLDARSALVVVSHDRT
                     FLNRLVTSIAEISDGRVTEWSGNWDAFRAQKDQARALAVKHAASEAHRVADVERFIER
                     FRYKATKARQVQSRIKMLEKMERTEVMAEEHTWRFTFPPVTRSAGRVALLSEVRKAYD
                     GRAVLKGLDLEIWRGDRVALVGPNGCGKSTLLRLVAGREPVDSGRIEIGEKVVTHYFA
                     QHVLETLQAGRTVLEELQAWSPGRTEGQLRSLLGIFQFSGDEVFKKVQVLSGGEKNRL
                     ALARLCLDPGNFLLLDEPTNHLDLPTREALEDALAGYGGTLLFVSHDRYFINRVATKV
                     AAFEDGRVRVYDGGYDDYLAAARGIAVTARAAAVARAGASPKPRPAPAPAAKQEALRQ
                     EPSDDRRTQRREEAETRNRRNRELRVFKDRIAKIESSILPLETRLKEIDQAMAEPDTW
                     HDPGKGKSLGEEKKALEIELAHLYDDWDHATTEMQAEEARLG"
     gene            complement(2346..3053)
                     /locus_tag="VFD06_05370"
     CDS             complement(2346..3053)
                     /locus_tag="VFD06_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZN02997.1"
                     /translation="MEKLPEFLIWYAVFLFTLAFHEGSHALIAYWGGDDTAYRGGQVS
                     LNPWPHIQREPFGTVLFPILTFFMSGWMMGWASAPFDPAWGARHPRRMAAMSAAGPAA
                     NLLLAVISFGILKALLGTGYFEAPTRINFSHMVEPSASGAESYGYAVGLLLSVALNLN
                     VLLGLFNLLPIPPLDGAGIASGLAPRTIGEGLERLNMNPAFRIMGLLIAWQVFGPIAR
                     IAFKGVLSMLHPELSYG"
     gene            complement(3062..3271)
                     /locus_tag="VFD06_05375"
     CDS             complement(3062..3271)
                     /locus_tag="VFD06_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HZN02998.1"
                     /translation="MSEHVYKSIELTGSSKKSIEDAVEKALARAGKTLRSMRWFEVLS
                     TRGYIEKGKLAYWQVSIKVGFTLED"
     gene            3317..3613
                     /locus_tag="VFD06_05380"
     CDS             3317..3613
                     /locus_tag="VFD06_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN02999.1"
                     /translation="MQRVRWVLIGIAIAFAFAAAIRSARMHGTGLPEPVDAAVGRPVA
                     ENRAERLTGEPPPPSARRAPEFPRSDADGWINSPPLSMSGLRGKVVLLDVWTYG"
     gene            3632..3955
                     /locus_tag="VFD06_05385"
     CDS             3632..3955
                     /locus_tag="VFD06_05385"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZN03000.1"
                     /translation="MPWVASIAEQFGSRGLHVVGIHTPEFEQEKDPALVRAAVARLKV
                     PYPVVLDNDETMWDALGTYAWPSLYLVDRKGAVRVEHVGEIHAGTKDAWHLEAQIESL
                     LRESA"
     gene            complement(3952..4584)
                     /locus_tag="VFD06_05390"
     CDS             complement(3952..4584)
                     /locus_tag="VFD06_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZN03001.1"
                     /translation="MSREIFFCVDIEASGPVPALYNMVSIGALAVSKREHRWVPEEER
                     FYIELKPMAAGFDPGAMKVHGITREHLEAHGVAPDEAMRRLAAFVEERLRKTGGRAVF
                     VGHNAAFDWSYISWYYERFSIPNPFGYKALDSKSLAMGRLGIGWSETNKERLEELLYL
                     PPQDKSQTHRADYDAYYQALILCGLLNRKIVASLTPPPLRTRPGGRRGPT"
     gene            complement(4599..4859)
                     /locus_tag="VFD06_05395"
     CDS             complement(4599..4859)
                     /locus_tag="VFD06_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HZN03002.1"
                     /translation="MKPLGGKRVFLRFEDGVSGEIDLAGRIEFTGVFAPLVDEREFAK
                     VRVNPDLGTIVWPNGADLAPETLYSWLTEAPVAGSDPESKVQ"
     gene            complement(4884..5144)
                     /locus_tag="VFD06_05400"
     CDS             complement(4884..5144)
                     /locus_tag="VFD06_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HZN03003.1"
                     /translation="MPEISRFFGIIITMYYNEHVPPHFHARYGGERASIGIESLQVLD
                     GSLPPRALGLVMEWASAHRSELMNDWFLAQSRCPLQPIDPLR"
BASE COUNT          882 a         1795 c         1838 g          784 t
ORIGIN      
        1 tgccgcgaca gcgccaggag cgactcgaga tcggcggcga tgcgggagaa cggctccggg
       61 cgccacggga gaggccggga gaatcggggc gcgagcgatc ggacctgcgc cggggacggg
      121 acgatcggat cgcgcccgcg acgcgagcgc cagccggcga gaagcctctt caccagccgt
      181 tccagccggt cggtggagtc gtcggcccgg tcgagacggc ggcgacgagg aggtgccttc
      241 gccggccggg gtgcgttcgc catgagccgc gcatcatgcc acagggcgca tcaggagccg
      301 gcgggcggcg gcttgcaagt aaaatcaccg gatgctgcga ttgtccgggg tgacgcgcgc
      361 ctacggcgtg cacgaggtgc tcaaaggggt ggactggggc gtcccgccgc gcgcccgcgt
      421 cgggctcatc ggcccgaacg gctccggcaa gaccaccctc ctccgcgtca tcgccggccg
      481 cgaggagccg gacaagggga cgatcaccct cacctccggc ctcaccctcg gctatctggc
      541 gcaggaaggg gcgcgcctgg ccgaagggac ggtgctcgac gccctcctcg cgccgttcga
      601 gaaggtggcg gcgctcgagc gcgagctgga ggcgctgcac cacgagatgg cgacggcgca
      661 gggggagcgg atggacacgc tgatccgccg cgccggggac gtgcagcacc gcttcgaggc
      721 ggccggcggc ttcacgctcg agacccaggc gaagacgatc ctcggggggc tcggcttcgc
      781 gcccgacgat cacgcgcgtc ccttgaagga gttctccggc gggttccgga tgcgcgccgc
      841 gctcggagcg ctgctgatgc agcagccgga ctatctcctc ctcgacgagc cgacgaacca
      901 cctcgatctc gaggcggtgc agtggctgga gcagttcctc ctcgacgccc gctcggcgct
      961 cgtcgtcgtg tcgcacgacc ggaccttcct gaaccggctg gtcacatcga ttgcggagat
     1021 ctccgacggg agggtgaccg agtggtccgg caactgggac gccttcaggg cgcagaagga
     1081 ccaggcgcgg gcgctggcgg tgaagcacgc cgcgagcgaa gcgcaccggg tggccgacgt
     1141 cgagcggttc atcgagcgct tccgctacaa ggcgaccaag gcgcgccagg tgcagagccg
     1201 catcaagatg ctggagaaga tggagcgcac cgaggtgatg gcggaggagc acacctggcg
     1261 cttcaccttc ccgcccgtga cccgctccgc cggccgtgtg gcgcttctct ccgaggtgcg
     1321 caaggcgtac gacggccgcg ccgtcctcaa ggggctcgac ctcgagatct ggcgcggcga
     1381 ccgggtggcg ctcgtcggcc cgaacggctg cggcaagagc acgttgctgc gcctggtggc
     1441 gggacgcgag ccggtcgact cagggcggat cgagatcggc gagaaggtcg tcacccacta
     1501 cttcgcccag cacgtcctcg agacgctgca ggcgggccgg accgtcctcg aggagctgca
     1561 ggcgtggtcg ccggggcgca ccgaggggca gctgcgctcg ctcctcggaa tcttccagtt
     1621 ctccggcgac gaggtgttca agaaggtcca ggtgctgagc ggcggggaga agaaccggct
     1681 cgctctggcg cggctctgcc tcgacccggg gaacttcctg ctgctcgacg aaccgaccaa
     1741 ccacctcgat ctcccgaccc gcgaggcgct cgaggacgcg ctcgccgggt acggcgggac
     1801 gctcctgttc gtgtcgcacg accgctactt catcaaccgc gtggcgacga aggtggcggc
     1861 gttcgaggac ggccgggtgc gcgtgtacga cggcggctac gacgactacc tcgcggcggc
     1921 gcgcgggatc gccgtgacgg cgcgggccgc ggcggtggcc cgggccggtg cctccccgaa
     1981 gccccgtccg gcgcccgcgc ccgcggcgaa acaggaggcc ttgcgccagg aaccctcgga
     2041 cgatcggcgg acccagcgcc gcgaggaggc ggagacgcgc aaccggagga accgcgagct
     2101 gcgcgtcttc aaggaccgga tcgcgaagat cgaatcgtcg atcctgccgc tcgagacgcg
     2161 cctcaaggag atcgaccagg cgatggccga gccggacacc tggcacgacc cgggcaaggg
     2221 gaagagcctc ggcgaggaga agaaggcgct cgagatcgag ctggcccacc tgtatgacga
     2281 ctgggaccac gccacgaccg agatgcaggc ggaagaagcg cgtctcggct gacccccctc
     2341 ggacttcagc cgtacgacaa ttccggatgc agcatcgaca gcaccccctt gaaagcgatg
     2401 cgggcgatcg ggccgaagac ctgccacgcg atgagcagcc ccatgatccg gaaggccggg
     2461 ttcatgttga ggcgctccag gccctccccg atcgtcctcg gcgccagccc ggacgcgatg
     2521 ccggcgccgt cgagcggcgg gatcgggagc aggttgaaca ggccgagcag gacgttgaga
     2581 ttgagcgcca ccgacagcag cagccccacc gcgtagccgt aggactcggc gccgctggcg
     2641 ctgggctcga ccatgtggct gaagttgatc cgggtgggcg cctcgaagta gccggtgccg
     2701 agcagcgcct tgaggatccc gaaggagatg accgcgagca ggaggttggc cgccggcccg
     2761 gcggccgaca tcgccgccat gcgccgcgga tgcctcgccc cccaggctgg atcgaacggg
     2821 gccgaggccc atcccatcat ccatcccgac atgaagaagg tcaggatcgg gaacagcacg
     2881 gtgccgaacg gctcccgctg gatgtgcggc cagggattga gcgacacctg gccgcctcgg
     2941 tacgccgtgt cgtccccgcc ccagtacgcg atgagcgcgt gcgacccttc gtggaaggcc
     3001 agggtgaaca ggaagacggc gtaccagatg agaaactccg ggagtttctc cacgtcgccg
     3061 ctcagtcctc gagcgtgaat ccgactttga tggagacctg ccagtaggcg agcttcccct
     3121 tctcgatgta gccgcgcgtc gagagcacct cgaaccagcg catgctgcgg agcgtcttgc
     3181 cggcgcgggc gagcgccttc tcgaccgcgt cctcgatgct cttcttcgag gatcccgtca
     3241 gctcgatcga cttgtagacg tgctcgctca tggcatcggt cctcccggtc caccgtgggc
     3301 cgtgtattct agacccatgc agcgtgtccg gtgggtgctc atcgggatcg cgatcgcctt
     3361 cgctttcgcg gcggccatcc gctcggcccg catgcacgga accggactgc cggagccggt
     3421 cgatgcggcc gtcggccgcc cggtcgccga gaaccgggcc gaacgcctga cgggcgagcc
     3481 ccctccgccg tccgcccggc gggcgccgga gtttccgcgc tccgacgccg acggctggat
     3541 caactccccg cccctgagca tgagtgggct ccgcggcaag gtggtcctcc tcgacgtctg
     3601 gacctacggc tgaatcaact gcacacgagc gctgccgtgg gtggcgtcga tcgccgagca
     3661 gttcggaagc cgcggcctgc atgtcgtggg tatccacact cccgagttcg agcaggagaa
     3721 ggacccggcc ctggtgcgtg ccgctgtcgc gcgcctgaag gtcccctacc cggtcgtgct
     3781 ggacaacgat gagacgatgt gggacgcgct cggcacctac gcctggccga gcctctacct
     3841 cgtcgatcgg aagggcgccg ttcgggtcga gcacgtgggg gagatccacg cgggcacaaa
     3901 agacgcgtgg catctcgaag cgcagatcga gagcctgctg cgggagtccg cctaggtggg
     3961 gccccgccgg ccgcccggcc tcgtcctcag cggcggaggc gtcagggagg cgacgatctt
     4021 cctgttgagc agaccgcaga ggatcagcgc ctggtagtag gcgtcgtagt cggctcgatg
     4081 cgtctgcgac ttgtcctgcg ggggaagata cagcagctcc tcgaggcgct ccttgttcgt
     4141 ctcggaccag ccgatgccga gccgccccat cgccagcgac ttgctgtcga gcgccttgta
     4201 cccgaacggg ttcgggatcg agaagcgctc gtagtaccag gagatgtacg accagtcgaa
     4261 ggcggcgttg tgcccgacga acaccgcccg acctccggtt ttcctgagcc gctcctcgac
     4321 gaacgccgcc agacgccgca tcgcctcgtc cggcgcgacg ccgtgcgcct ccaggtgctc
     4381 gcgggtgatc ccgtgcacct tcatcgctcc cggatcgaac cccgccgcca tcggcttcag
     4441 ctcgatgtag aagcgttctt cttccggcac ccagcgatgt tcccgcttcg atacggccaa
     4501 cgcgccgatg gagaccatgt tgtacagcgc cggcaccggc ccggaggcct cgatgtcgac
     4561 gcagaaaaag atctcccggg acacttcttg cggccctcct actgaacctt gctctccgga
     4621 tcggacccag ccactggggc ttcagtcagc cacgagtaaa gcgtctccgg agcgagatcg
     4681 gcgccattgg gccagacgat cgtgccaagg tccggattca cgcggacctt tgcgaactcc
     4741 cgctcatcga ccagcggggc gaagactccg gtgaactcaa tccgcccggc gagatcgatc
     4801 tcaccggaca ccccgtcctc gaagcggaga aacacccgct tccctccgag cggcttcact
     4861 tcgacgacat cccggagcac caattacctc agcggatcga taggctgcag cggacagcgg
     4921 gactgcgcga ggaaccaatc attcatgagc tcgctcctgt gcgccgatgc ccattccatc
     4981 acgagtccga gtgcccgggg cggtagcgag ccgtccagaa cctgaagcga ctcgatcccg
     5041 atgcttgctc gttccccgcc atagcgtgca tggaaatgcg ggggaacgtg ctcgttgtaa
     5101 tacatggtga tgatgattcc gaagaacctg ctgatctcgg gcaaggtgcc ccctcacagg
     5161 ctctccgagc aagattctag cccaggagcg tgtctgggga cttcgtcaga cgggctactg
     5221 gaggcgcggc ctctggtcca tccgggcgat ccaggcgagg aggttcgggt ggttcgtgcc
     5281 gggccgctgg aagtcgcgc
//
LOCUS       DASRIQ010000216         6335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_861716, whole genome shotgun sequence.
ACCESSION   DASRIQ010000216 DASRIQ010000000
VERSION     DASRIQ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861716"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFD06_05405"
     CDS             complement(<1..601)
                     /locus_tag="VFD06_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03004.1"
                     /translation="MTEVTIGGYVASSAALQGAAGRAQAIAGLCEQMAAAGLAIAREV
                     RRAALTGRTGYAGGANVSGDRQKKLDVVGNALVLDAFRKSQHVAAIVSEEDVQPVPTE
                     HPEAPYVLSTDPIDGSSNTDIDGPLGTIFSFSTRRPDGGMPRGGDIVAAGYVLYGPST
                     ICAVSAGAGAAAFTLDEERSAWVRTHPELRCPERGSTFSA"
     gene            complement(627..1598)
                     /gene="glk"
                     /locus_tag="VFD06_05410"
     CDS             complement(627..1598)
                     /gene="glk"
                     /locus_tag="VFD06_05410"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HZN03005.1"
                     /translation="MILAGDVGGTKTRLALFDTEQGGGRLRARNERTYPSREHRNLEA
                     IVARFQRDVPAPIERAAFGVAGPVRDGVCVTTNLPWVVEAASLARLLRLSGSGLLNDL
                     EATAWGLAELREEDTVTLQAGAAGAAGNSAIIAAGTGLGEAGLFWNGRRHVPFATEGG
                     HADFAPRSTIEDELLRHLRARYDRVSWERVVSGPGLVEIHDFLSGRDGTKTVEVDSGE
                     DRGPEITRAALAGTSRVASEALDLFVSAYGAEAGNLALKMMATGGLFVGGGIAPRIID
                     RILAGGFMGAFLDKGRMRPLLQAMPVRVVLNDRTALLGAARQAAEMP"
     gene            complement(1540..3135)
                     /gene="zwf"
                     /locus_tag="VFD06_05415"
     CDS             complement(1540..3135)
                     /gene="zwf"
                     /locus_tag="VFD06_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZN03006.1"
                     /translation="MSTGDLVQGSWPQHRPGPGDPCILCIFGATGDLTRRKLLPALLN
                     LSRGGLLPAQVGIVGVGREPLDDATFRTRIAEGLAEFATPEIPPQAREDLIRRMRYLS
                     GDFADPGLYRALKDLLAEVDAKEKTRGNVLYYLATPPSVFAPIVHALGAAGMVAENGG
                     WRRVIVEKPFGEDLESAKGLNLELRRDLDERQIFRIDHYLGKETVQNIMVFRFANGIF
                     EPVWNRRYIDHVQITVAESDGIGTRGRYYDQFGALRDMVPNHLFQLLSFIAMEPPISF
                     EAGAVRDEKAKVLRAIAPMTEEEVLRRTVRGQYKDGLGPEGRRHPGYRTEEYVKSGSS
                     TETFVALKLYVDNWRWAEVPFYLRTGKGLAERVTEIVIQFKRAPFVLFRDTEIERLPA
                     NTLVLNIQPDEGISLRFQAKVPGPTVRMGEVEMDFKYAEHFGEVPSTGYETLLHDCMV
                     GDATLFQRDDMVEAGWRIVQPILDVWKALPPRNFPNYPFGSSGPREADELMQRDGRKW
                     KAIGDDPGGRRRGNQDPAGAVRH"
     gene            complement(3132..4154)
                     /gene="gnd"
                     /locus_tag="VFD06_05420"
     CDS             complement(3132..4154)
                     /gene="gnd"
                     /locus_tag="VFD06_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HZN03007.1"
                     /translation="MKIGMIGLGRMGANMVRRLMRAGHPCVVHARNPESVRALVAEGA
                     EGAFSLRELVGNVPRPRAVWVMIPAGAPTEETVKELGTLLSQDDVVIDGGNSFFKDDL
                     RRAAALKERGIHYIDVGTSGGVWGVERGYCLMIGGPRAAVERLDPIFKTLAPGRAAAP
                     PTPGRPRRSDHPVTSSDVTVPSTAEEGYLHCGPSGAGHFVKMIHNGIEYGAMQAYAEG
                     FDIMKSASRDTVPEAQRFDLDVAEIAELWRRGSVVSSWLLDLTAQALAASPTLEEFEG
                     VVADSGEGRWTVQTAIEQAVPADVLSAALYARFRSRQAHTFAEKILSAMRAGFGGHIE
                     PGKRHP"
     gene            complement(4151..>6335)
                     /locus_tag="VFD06_05425"
     CDS             complement(4151..>6335)
                     /locus_tag="VFD06_05425"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HZN03008.1"
                     /translation="GADKTRLERLLGKVAIANGKQTYRTYKEIFSGPRWKALEAKGAQ
                     TQRVLWASTSTKNPAYRDVMYVEDLIGPDTVNTLPPATLDAFRDHGEARATIEAGLDE
                     ADRTLRDLEAAGLSLKKITDQVLDEGVRLFADAFDGLLSAVEKKRREILGPRLARQTA
                     SLPDALESEVQSTLEEWRRNGNVRRLWSGDASLWTGGDEAKWLGWLESPWEMRRAAPA
                     LMAAGREVRGAGFTHALLLGMGGSSLAPEVAVATLGPAQGAPRLVVLDSTDPAQIRTA
                     DQQTDAGRTLHIVASKSGTTLEPAIFRDYFLDRARKGLAEGAGGRFVAITDPGSELEA
                     QARRDRFRAVVPGIPSVGGRYSALTNFGLFPAAVAGIDVAALLDRALMLARSCSASVP
                     PAENPGVILGTVLGVLGRRGRDKVTFVVSPGLATLGAWLEQLLAESTGKNGQGLIPIA
                     LETPGPPHDYGADRLFVYIRLEAAPDAAQDQAVTEIGKAGHPVVRIEVPSKEDLGGEW
                     FRWEIATAVAGAVLGLNPFDQPDVEASKVATRLLVSEYEKTGALPQETPVASGDGLRL
                     FADARNGRSLLEASGANASVATVLKAHLARLGAGDYFALLAYLEMSPHHVDALQQIRH
                     RVRRARKVATCLGFGPRFLHSTGQAYKGGPPSGVFLQITCDDAKDLPIPGRKATFGIV
                     KAAQARGDFQVLAERGRRALRVHLGADVATGLETLGHLVERALGA"
BASE COUNT          871 a         2254 c         2289 g          921 t
ORIGIN      
        1 tggcgctgaa cgtcgagccg cgctcggggc agcgcagctc cgggtgggtc cgcacccagg
       61 cgcttcgctc ctcgtcgagc gtgaaggcgg ccgccccggc gccggcgctc acggcgcaga
      121 tcgtgctcgg accgtagagc acgtaccccg cggccacgat gtcgccgccg cgcggcatcc
      181 cgccgtccgg gcgccgcgtc gagaaggaga agatggtccc gagcgggccg tcgatgtcgg
      241 tgttcgagga gccgtcgatc gggtcggtgg agaggacgta cggcgcctcc ggatgctctg
      301 tcggcaccgg ctgcacgtcc tcctcgctga cgatggcggc gacgtgctgg ctcttccgga
      361 aagcgtcgag gaccagcgcg ttgccgacga cgtccagctt cttctgtctg tcgccgctga
      421 cgttggcgcc gccggcgtag ccggtgcgcc cggtcagggc cgcgcgccgg acctcccggg
      481 cgatggcgag ccccgcggcc gccatctgct cgcagagccc ggcgatcgcc tgcgcccggc
      541 cggcggcgcc ctgaagggcc gcgctcgaag cgacgtagcc tccgatggtg acttccgtca
      601 cagtgcctcc cgtagggaca tccccatcac ggcatctccg ccgcctgacg cgccgccccc
      661 agaagggcgg tccggtcgtt gaggacgacg cggaccggca tcgcctggag cagcggccgc
      721 atgcgcccct tgtcgaggaa cgcccccatg aagccgccgg cgaggatccg gtcgatgatg
      781 cgcggcgcga tgccgccgcc gacgaagaga ccgccggtgg ccatcatctt gagggcgagg
      841 ttccccgcct cggccccgta ggcggacacg aacaggtcga gcgcctcgga ggcgacccgg
      901 ctggtgcctg cgagcgcggc gcgggtgatc tccggcccac ggtcctcccc ggaatcgacc
      961 tcgaccgtct ttgtgccgtc ccggccggag aggaagtcgt ggatctcgac gagacccggg
     1021 ccggagacga ccctttccca cgacaccctg tcgtaacggg cgcgaagatg gcgcaacagc
     1081 tcgtcctcga tggtggagcg gggggcgaag tcggcatgcc ccccctcggt cgcgaagggg
     1141 acgtggcggc ggccgttcca gaaaagcccg gcctccccca gtcccgtgcc ggcggcaatg
     1201 atagcggaat tccccgccgc ccccgccgcc cctgcctgca gcgtgacggt gtcctcctcc
     1261 ctcagctcgg ccaaccccca ggcggtcgcc tcgagatcgt tcaggaggcc gctcccggaa
     1321 agacggagaa gcctcgcgag gctcgccgcc tcgacgaccc aggggaggtt ggtggtgacg
     1381 cagacgccgt cccggacggg acccgcgacg ccgaaggcgg cccgctcgat cggggccggg
     1441 acgtcgcgct ggaagcgggc gacgatcgcc tcgaggttgc ggtgctcgcg gctcgggtag
     1501 gtgcgctcgt tccgcgcgcg cagccgcccg ccgccctgct cagtgtcgaa cagcgccagc
     1561 cgggtcttgg ttcccccgac gtcgcccgcc aggatcatcg ccgatggcct tccacttgcg
     1621 accgtcgcgc tgcatcagct cgtccgcctc gcgggggccg ctgctgccga acgggtagtt
     1681 ggggaagttg cgcggcggca gcgccttcca gacgtcgagg atcggctgga cgatgcgcca
     1741 gccggcctcc accatgtcgt cgcgctggaa cagcgtggcg tcgccgacca tgcagtcgtg
     1801 caggagcgtc tcgtagccgg tggaggggac ctcgccgaag tgctcggcgt acttgaagtc
     1861 catctccacc tcccccatgc gcaccgtcgg tccgggcacc ttcgcctgga accgcaggga
     1921 gatcccctcg tccggctgga tgttgaggac gagggtgttg gccggcaggc gttcgatttc
     1981 cgtgtcgcgg aacaggacga acggggcccg cttgaactgg atgacgatct cggtcacccg
     2041 ctcggccagc cccttgccgg tgcgcagata gaacggcacc tccgcccagc gccagttgtc
     2101 cacgtagagc ttgagcgcca cgaaggtctc ggtggaggag ccggacttga cgtactcctc
     2161 ggtccggtac ccggggtggc gccgcccctc cggcccgagg ccgtccttgt actggccgcg
     2221 cacggtgcgg cgcagcacct cctcctcagt catcggagca atggcgcgca ggaccttcgc
     2281 cttttcgtcc cgcaccgccc cggcttcgaa ggagatcggc ggctccatgg cgatgaacga
     2341 cagaagctgg aacaggtggt tcggcaccat gtcccggagc gcgccgaact gatcgtagta
     2401 gcgaccccgg gtcccgatgc cgtcgctctc ggcgacagtg atctggacgt ggtcgatgta
     2461 gcggcggttc cagaccggct cgaagatccc gttggcgaac cggaacacca tgatgttctg
     2521 gaccgtctcc ttgcccaggt agtgatcgat gcggaagatc tggcgctcgt cgaggtcgcg
     2581 ccgcagctcc aggttgagcc ccttcgccga ctcgaggtcc tcgccgaacg gcttctcgac
     2641 gatcaccctc cgccagccgc cgttctcggc gaccatcccg gcggcgccga gggcgtgcac
     2701 gatcggcgcg aagaccgacg gcggcgtcgc gaggtagtag aggacgttgc cgcgggtctt
     2761 ctccttcgcg tccacctcgg ccagcaggtc cttcaacgcc cggtacagcc cggggtcggc
     2821 gaagtccccc gacaggtagc gcattctgcg gatcaggtcc tcgcgcgcct gcggcgggat
     2881 ctccggcgtg gcgaactcgg cgagcccctc ggcgatgcgg gtgcggaacg tcgcgtcgtc
     2941 cagcggctcg cgccccacgc cgacgatccc gacctgcgcc ggcagaagcc ccccgcgcga
     3001 caggttgagc agggcgggga ggagcttgcg gcgcgtcagg tcgccggttg cgccgaagat
     3061 gcagaggatg caggggtcgc cggggcccgg ccggtgctgc ggccagctcc cctggacgag
     3121 gtctccggtg ctcacgggtg gcgtttcccc ggctcgatgt ggccgccgaa gccggcgcgc
     3181 atcgccgaga ggatcttctc ggcgaaggtg tgggcctggc gcgagcggaa gcgggcgtag
     3241 agggccgccg acaggacgtc ggccggcacc gcctgctcga tggcggtctg caccgtccag
     3301 cgcccctcgc ccgagtccgc gacgacccct tcgaactcct cgagcgtcgg gctcgccgcc
     3361 agcgcctggg cggtcaggtc gaggagccac gacgacacga cgctgccgcg ccgccacagc
     3421 tcggcgatct cggccacgtc gaggtcgaag cgctgcgcct cggggaccgt gtcgcgcgaa
     3481 gcgctcttca tgatgtcgaa cccttcggcg taggcctgca tggcgccgta ctcgatgccg
     3541 ttgtggatca tcttcacgaa gtggccggcc ccggacgggc cgcagtgcag gtacccctcc
     3601 tcggcggtgg aagggacggt gacgtccgag gacgtcaccg ggtgatcgga gcggcgcggg
     3661 cgtcccgggg tcggcggggc ggcggcgcgg ccgggggcga gcgtcttgaa gatcgggtcg
     3721 agacgctcga ccgcggcgcg cgggccaccg atcatcaggc agtagccgcg ctcgacgccc
     3781 cagacgccgc cgctcgtgcc gacgtcgatg tagtggatgc cgcgctcctt gagcgccgcg
     3841 gcgcggcgca gatcgtcctt gaagaaggag ttgccgccgt cgatgacgac atcgtcctgg
     3901 gagaggagcg tcccgagctc tttcaccgtc tcctcggtgg gagcgccggc gggaatcatg
     3961 acccagacgg cgcgagggcg ggggacattg ccgacgagct cgcgcagcga gaacgcccct
     4021 tcggcccctt cggcgacgag cgcgcggacg ctctcgggat tgcgggcgtg gacgacgcac
     4081 gggtgaccgg cgcgcatcag gcggcggacc atgttcgcgc ccatgcggcc gaggccgatc
     4141 atcccgatct tcatgcgccg agggcccgtt cgacgagatg gccgagcgtc tcgaggccgg
     4201 tggcgacgtc ggcgccgagg tgcacgcgca gggcgcgccg gccgcgctcg gccagaacct
     4261 ggaagtcgcc gcgcgcctgg gccgctttca cgatcccgaa cgtcgccttg cgtccgggga
     4321 tcggcaggtc cttcgcgtcg tcgcaggtaa tctggaggaa gacgccgctc ggtggcccgc
     4381 ctttgtaagc ctggccggtg gagtgcagga agcgcggccc gaacccgaga caggtggcga
     4441 ccttgcgggc gcgccgcacc cggtggcgaa tctgctgcag cgcgtccacg tggtgcgggc
     4501 tcatctcgag atacgcgagg agcgcgaagt agtcgcccgc gccgagccgg gcgagatgcg
     4561 ccttgaggac cgtcgccacg gacgcgttcg cgccggacgc ctcgaggagc gaccggccgt
     4621 tgcgggcgtc cgcgaagagc ctcaggccgt cgccagaggc gaccggcgtc tcctggggga
     4681 gcgccccggt cttctcgtac tcgctcacga gcagacgcgt cgccaccttg ctcgcctcga
     4741 catccggctg atcgaagggg ttcagcccca ggaccgcgcc ggcgacggcg gtggcgatct
     4801 cccagcggaa ccactcgccg ccgaggtctt ccttcgaggg gacctcgatc cgcaccaccg
     4861 ggtgcccggc cttgccgatc tccgtgaccg cctgatcctg ggcggcgtcg ggggccgcct
     4921 cgagccggat gtacacgaac agccgatcgg cgccgtagtc gtgcggcggg ccgggggtct
     4981 cgagggcgat cgggatgagc ccctgcccgt tcttgccggt ggactcggcg aggagctgct
     5041 cgagccacgc gccgagcgtc gcgagcccgg gggagacgac gaaggtgacc ttgtcccgac
     5101 cgcggcgacc gagcaccccg agcaccgtcc cgaggatcac gcccgggttc tcggccgggg
     5161 gcaccgacgc ggagcacgag cgcgcgagca tcagggcgcg atcgagaagc gccgccacgt
     5221 cgatcccggc gaccgccgcg gggaacaggc cgaagttcgt cagcgccgaa tagcggccgc
     5281 ccaccgacgg gatgccgggg accaccgccc ggaagcgatc gcgccgcgcc tgcgcctcga
     5341 gctcggatcc ggggtcggtg atggcgacga agcggccgcc cgccccctcc gcgagcccct
     5401 tgcgggcgcg gtcgaggaag taatcgcgga agatcgccgg ctcgagcgtc gtgcccgact
     5461 tgctggcgac gatgtggagc gtcctcccgg catcggtctg ctgatcggcc gtccggatct
     5521 gcgccggatc ggtgctgtcg agcaccacga ggcggggtgc gccctgcgcc ggaccgaggg
     5581 tcgccaccgc cacttccgga gcgaggctcg atccccccat gccgaggagg agcgcatggg
     5641 tgaagccggc gccgcggacc tcgcgcccgg ccgccatcag ggccggggcg gcgcggcgca
     5701 tctcccacgg gctctcgagc cagccgagcc acttcgcctc gtccccgccg gtccacagcg
     5761 atgcgtcgcc gctccacagc cgccgcacgt tcccgttgcg gcgccactcc tcgagcgtgg
     5821 actgcacctc cgattcgagg gcatccggga ggctcgccgt ctggcgggcc agccgcggtc
     5881 cgaggatctc gcgccgcttc ttctcgaccg cggagaggag cccgtcgaag gcgtcggcga
     5941 agaggcgcac cccttcgtcg aggacctggt cggtgatctt cttcagcgac agcccggcgg
     6001 cctcgaggtc gcgcagcgtc cggtccgcct cgtcgagccc cgcctcgatc gtggcgcgcg
     6061 cctcgccgtg atcgcggaag gcgtcgaggg tggcgggcgg cagcgtgttg acggtgtcgg
     6121 ggccgatcag gtcctcgacg tacatgacgt cgcggtacgc ggggttcttc gtgctggtcg
     6181 aggcccacag gacgcgctgc gtctgcgccc ccttcgcttc gagcgccttc cagcgcgggc
     6241 cggagaagat ctccttgtac gtcctgtacg tctgcttgcc gttggcgatg gcgaccttcc
     6301 cgagcagccg ctcgagacgc gtcttgtcgg cgccg
//
LOCUS       DASRIQ010000217         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3483353, whole genome shotgun sequence.
ACCESSION   DASRIQ010000217 DASRIQ010000000
VERSION     DASRIQ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3483353"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="VFD06_05430"
     CDS             <1..1400
                     /locus_tag="VFD06_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZN03009.1"
                     /translation="SGMRKFIFSALLLAGLVAIVAPVAFAQEEKPFVLHGEVRTRFEY
                     TNNANDFADADGPLGTGDDQANYWPYRIRIAAEGHFTKNISAWIEFQNAGVFGGDFTG
                     PVKNGTVFGFAADSGVSLYQGNLTLDNLWSESFSLTFGRSELVKGNELHLGDLDFYAG
                     IVHDGVIGNWDFKKVDITGWYTRPIEGSVFHPDNAIPPGAVLVSGTPVTQHFWGAYVT
                     WDVATEQGLDLYFMEARNRDFTGPDSPTTLDMIGVRWFHDNTTKNGPVWNGEVAQQFG
                     EISDLSTPGAFTAGADLSGMVIEAMFGWNFKTGDIIHRPYGKFESASGNETEVGGDDE
                     DEGFRPFFTDFHNRLGHGDWFTLSGGDPSFVGAGANGGTGITAVSLGYTGWGTRHSWG
                     AEFWDYTLDQDAVIGGVEEDDLGTAFDLWYGFNYSKNLVFEAALSQLSPGDALTAGGP
                     DDSAMRFYGQARLRF"
     gene            complement(1456..2745)
                     /gene="aroA"
                     /locus_tag="VFD06_05435"
     CDS             complement(1456..2745)
                     /gene="aroA"
                     /locus_tag="VFD06_05435"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZN03010.1"
                     /translation="MSAPPDRGGARVIRPGGRIAARLQAPPSKSATQRALVIASLARG
                     TSRILRPLLGEDGALLRDALAACGVPITTGVALEVQGGDAARGAGTTLSLGNAGTAMR
                     FLAARLAIEPDPRLLDGGARMRERPIEDLLAALRLLGMEAEAVRGNGCPPIRVGGGRP
                     RGGEVRLPGGRSSQFASALLMSAPRFEAGLSLAIEGEFVSRPYVRLTAALMRRFGVEV
                     GQEEQRFVVAAGQDYRPIGLEIEGDWSSASYHFAAAAIGGGRVEVAGLDQGSAQGDAG
                     ILELLRRMGCRAGTSGDGSIFVEGPERLSAIEADLRDMPDLAPTVAILGLFAEGPTRI
                     HGVPHLRLKETDRIAVLCEGIAALGGSAEPAADGLTVRPRPLAGGTVDPRGDHRMAMA
                     FAVAGLRVPGVVILDPDCVAKSYPGFWDDFDALSRDE"
     gene            2832..>4223
                     /locus_tag="VFD06_05440"
     CDS             2832..>4223
                     /locus_tag="VFD06_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03011.1"
                     /translation="MRHAPILPVLLALAAFGAPASAASEPPPQRAVQSAEDRGFAIVL
                     TSPTEPWLNGRQAIRIEAIIPRDDTVEQVDFFIDRKLAFVDTDEPYQHTFDFGAEIRR
                     HTIEVKALTKGGRRARVSFVSRSIDLAENAQGRIVTLTAMVRDTAGHPVDRLNVSDFS
                     VTEEGVRQPIVHFLAGPSPASMAVVVEPEAWIECRAGLERFLSTLPGHQAVALLGAPE
                     NTPLPAPAPEEPGEPAATPAVGKALPAAPVARAGSPFTYDLAALVASMPRGEAAPPEP
                     AAESPAVAKRPPSASLAATLTDRLVHAATALASRRGPRRLLLVAAVAPFGDEPIGPLL
                     PAVLPEAPKPGPAAVAGGQAEARPADPLAAALEEARKSGAAVHVIALPAPDRILWHEA
                     NAAALTLKAAVEASGGSWDEAADAAGLERAFASVTERLRNQYLVSYVSGAAAHPGWRP
                     LEVRTGGREWLVEA"
BASE COUNT          616 a         1558 c         1465 g          584 t
ORIGIN      
        1 catcgggaat gagaaagttc atcttcagtg cgctcctgct cgcgggcctc gtggcgatcg
       61 tcgccccggt cgccttcgcg caggaggaga agccgttcgt gctccacggc gaggtccgca
      121 cgcggttcga gtacaccaac aacgcgaacg acttcgcgga cgccgacggg ccgctcggca
      181 ccggtgacga ccaggccaac tactggccgt accgcatccg gatcgcggcc gaggggcact
      241 tcaccaagaa catcagtgcc tggatcgagt tccagaacgc cggcgtgttc ggcggcgact
      301 tcaccggccc ggtgaagaac ggcacggtgt tcggcttcgc ggccgactcc ggcgtcagcc
      361 tctaccaggg gaacctgacc ctggataacc tctggagcga gtccttcagc ctgaccttcg
      421 gccggagcga gctcgtgaag gggaacgagc tgcacctcgg cgacctcgat ttctacgccg
      481 ggatcgtcca cgacggcgtc atcggcaact gggacttcaa gaaggtcgac atcaccggct
      541 ggtacacccg cccgatcgaa gggagcgtct tccacccgga caacgcgatt ccgccggggg
      601 ccgtgctggt cagcggcaca ccggtgaccc agcacttctg gggtgcctac gtgacctggg
      661 acgtggcgac ggagcagggg ctcgacctct actttatgga ggcgcgcaac cgcgacttca
      721 ccggccccga ctcccccacc acgctcgaca tgatcggcgt ccgctggttc cacgacaaca
      781 cgacgaagaa cggcccggtc tggaacggcg aggtggcgca gcagttcggc gagatcagcg
      841 acctgtccac gcccggcgcc ttcaccgccg gcgccgatct ctccgggatg gtgatcgagg
      901 cgatgttcgg ctggaacttc aagaccggcg acatcatcca ccggccgtat gggaaattcg
      961 agtcggccag cggcaacgag accgaggtcg gtggcgatga cgaggacgag ggcttccgcc
     1021 cgttcttcac cgacttccac aaccgtctgg gccatggcga ctggttcacc ctgtccggcg
     1081 gcgacccgtc cttcgtcggc gccggcgcga acggcggcac cggcatcacc gccgtctcgc
     1141 tcggctacac cggctggggc acccggcatt cctggggcgc cgagttctgg gactacacgc
     1201 tcgaccagga cgcggtgatc ggcggcgtgg aggaggacga cctcggcacg gcgttcgacc
     1261 tctggtacgg cttcaactac tcgaagaacc tggtgttcga ggcggcgctg tcacagctgt
     1321 cgccgggcga tgccctgacc gcgggcgggc ccgacgactc agcgatgcgt ttctacgggc
     1381 aggcgcgact ccgcttctaa gcgcccaacg ttctggcagt tcccgacccc cgcggggttt
     1441 ccccgcgggg gttttttatt cgtcccggga gagcgcgtcg aagtcgtccc agaagccggg
     1501 gtacgacttg gcgacgcagt cgggatcgag aatgacgact cccgggacgc gcagcccggc
     1561 gaccgcgaag gccatcgcca tgcggtggtc gccgcgggga tcgacggtcc cccccgcaag
     1621 cgggcgcggc cggaccgtca ggccgtcggc ggccggttcg gccgacccgc cgagcgccgc
     1681 gatcccctcg cacagcaccg cgatccggtc ggtctccttg aggcgcaggt gcggcacgcc
     1741 gtggatccgg gtgggaccct ccgcgaacag gccgaggatc gcgaccgtcg gagcgaggtc
     1801 cggcatgtcg cgcaggtcgg cctcgatggc gctcaaccgt tccggtcctt cgacgaagat
     1861 gctcccgtcc ccggaggtcc cggcgcggca ccccatgcgg cgcagcagct cgaggatccc
     1921 cgcgtccccc tgcgcggagc cttgatcgag cccggcgacc tcgacccggc ctccgccgat
     1981 cgccgccgcc gcgaagtgat aggacgcgga cgaccagtcc ccttcgattt cgagaccgat
     2041 cggccggtaa tcctgtccgg cggccaccac gaagcgctgc tcctcctgac ccacctcgac
     2101 gccgaaacgg cgcatgaggg ccgcagtcag ccgcacgtag gggcgggaca cgaattcccc
     2161 ctcgatcgcg agcgacagcc cggcctcgaa gcgcggcgcg gacatgagca gcgccgaggc
     2221 gaactggctg ctccgcccgc ccggcaggcg gacctctccg ccgcgcggcc gcccgccgcc
     2281 gacgcggatc ggagggcagc cgttgccgcg caccgcctcc gcctccatcc cgaggaggcg
     2341 cagcgccgcc aggagatcct cgatcgggcg ctcccgcatc cgcgcgccgc cgtcgagcag
     2401 gcgcgggtcc ggctcgatcg ccaggcgtgc cgcgaggaag cgcatggcgg tgccggcgtt
     2461 gccgagcgaa agggtcgtgc cggcgccgcg cgccgcgtca ccgccctgca cttccagagc
     2521 gacgccggtc gtgatcggca caccgcaggc ggcgagcgca tcgcgcagga gcgccccatc
     2581 ctctccgaga agcggtcgca ggatacggga cgttccacgg gcgagcgagg cgatcaccag
     2641 ggcccgctgc gtggcgctct tggagggggg cgcctggagg cgggccgcga tccggccgcc
     2701 ggggcggatg acccgggcgc ctccgcggtc gggcggggcg ctcaccatcg gcgccggagg
     2761 cttgacgggg tcgccccgtc gtttctatgt tgtccgtgcc gggtcgtttc ggaccggcac
     2821 ggtgccccct gttgcgtcac gccccgatcc tcccggtcct tctggcgctc gccgccttcg
     2881 gtgcgccggc gtccgccgcc tcggagccgc cgccgcagcg agcggtgcag agcgccgaag
     2941 atcgcggctt cgccatcgtc ctcaccagcc cgaccgagcc gtggctgaac ggacgccagg
     3001 cgatccgcat cgaggcgatt atcccccgcg acgacaccgt cgagcaggtc gatttcttca
     3061 tcgaccggaa gctcgcgttc gtcgacaccg acgagccgta tcagcacacc ttcgacttcg
     3121 gcgccgagat ccgccgccac acgatcgagg tgaaggcgct gacgaagggg ggccgccgcg
     3181 cccgggtctc gttcgtgtcc cgctcgatcg acctggcgga gaacgcccag gggcggatcg
     3241 tcacgctgac cgcgatggtg cgggacaccg ccggacatcc ggtcgatcgt ctcaacgtca
     3301 gcgatttcag cgtgaccgaa gagggcgtcc ggcagccgat cgtgcacttc ctcgcggggc
     3361 cgagccccgc ctcgatggcg gtcgtcgtcg aaccggaagc ctggatcgaa tgccgcgccg
     3421 gcctggagcg gttcctttcg accctgccgg gacaccaggc ggtggcgctt ctcggagcgc
     3481 cggagaacac gccgctcccc gccccggctc ccgaggaacc gggcgagccg gcggccacac
     3541 cggctgtcgg gaaggcgctc ccggctgcgc ccgtcgcgcg ggcgggctcc cctttcacct
     3601 acgacctcgc cgctctcgtg gcgtcgatgc cgcgcggcga ggcggcgccc cccgagccgg
     3661 ccgcggaaag ccccgccgtg gcgaagcgcc ccccatccgc gtcgcttgcc gcgacactga
     3721 ccgatcggct cgtgcacgcg gcgacggcgc tcgcttcccg ccgcgggccg cggcggctgc
     3781 tcctcgtggc cgccgtcgcc ccgttcggcg acgaaccgat cggtccactg ctccccgcgg
     3841 ttctgcccga agccccgaag ccgggacctg ccgccgtcgc cggcggtcag gcggaggccc
     3901 gccccgccga ccctctcgcc gcggcgctcg aggaggcgcg gaaaagcggc gcggcggtgc
     3961 acgtcatcgc gctcccggcc ccggatcgca tcctgtggca cgaggcgaac gcggccgcgc
     4021 tcacgctcaa ggcggccgtc gaggcgagcg gcgggtcctg ggacgaggcg gccgacgccg
     4081 ccgggctcga acgggcgttc gcgtcggtta ccgaaaggct gcgcaaccaa tacctcgtga
     4141 gctacgtcag cggcgccgcg gcccaccccg gctggaggcc gctcgaggtc aggaccggcg
     4201 gccgcgaatg gctcgtcgag gcg
//
LOCUS       DASRIQ010000218         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3212996, whole genome shotgun sequence.
ACCESSION   DASRIQ010000218 DASRIQ010000000
VERSION     DASRIQ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3212996"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /gene="mnmE"
                     /locus_tag="VFD06_05445"
     CDS             <1..1421
                     /gene="mnmE"
                     /locus_tag="VFD06_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZN03012.1"
                     /translation="AGSGGIVSLPPREDTIVAIATPPGRGGVGAVRLSGEGAASIARR
                     LFRPSGAPTGPDRFEPGRALPGTFVLADGAPIDRGYLVLFAAPRSFTGEEVAELWTHG
                     SPPVLRALVDAAVSCGARPATPGEFSYRALLNRRIDATQAEAIRDLVEARTLHQARVA
                     HAQEAGALSRALRPLSARLADVAARVEAAIEFSEEPEVERADAEGGAAEILRSTLASL
                     QDLLVGFDRGRRLREGASVVLAGLPNAGKSSLFNRLVGSARAIVTPVAGTTRDVVEET
                     IEIGGVPVTLADTAGLHDAANEADREAVQRARAAAAGADIVLVVLDWSRPWRDADRET
                     IEAAGADRALVVLNKVDLPCRVGLDRILSLRKRNAALEVSAGTGEGVEDLRRLLLERI
                     APLGARQPDEIFLTSVRQKDLVARAAAAIERALQAGRERRGLECVAVDLRDALDRLGE
                     LSGEIHLDDLYDRLFSTFCIGK"
     gene            1418..>3268
                     /gene="mnmG"
                     /locus_tag="VFD06_05450"
     CDS             1418..>3268
                     /gene="mnmG"
                     /locus_tag="VFD06_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZN03013.1"
                     /translation="MIASRADVVVVGAGHAGLEAALAAARMGCSTLLVTLAREATGRM
                     SCNPAIGGLAKGHLVREIDALGGEMGRAIDATGIQFRLLNASRGPAVQAPRAQADKEA
                     YARRMRETVERTERLGLVEDEVIDLAVEGGRITGVILRRAGRVDAAAVVVTSGTFLRG
                     LIHIGSSQRAGGRIHEAAAEGLSAALQRLGLPLGRLKTGTPPRILKDSVDRTRLERQP
                     GDKEPVPFSFETGRIDRPQVDCHITWTSPETHRLIRANLDRSPLYTGVIRSVGPRYCP
                     SIEDKVVRFADRERHQIFVEPEGIDHPWLYLNGLSTSLPEEVQQPLVRTLPGLEEAVV
                     ARPGYAVEYDFVPPTACRHTLETKTVAGLFLAGQINGTTGYEEAAALGLLAGVNAGLQ
                     AQGRPPFVLSRGAAYAGVMVDDLVTRGTSEPYRMFTSRAECRLLLDIDTADLRLTPYG
                     RDLGLIGAGRFARFEERRRRIAAWSAVLETRAILPNAATNERARRDLGIVLNEPTTAA
                     RLLRRSDVTAEGIERFLAEDLPRGLTVRERNTVANRLRYGGYIERQERHVDRLRREEG
                     RRIPDDFDYRAIAGLSREVVEKLTIARPGTLREAGLISGVTPAALVLVNVA"
BASE COUNT          434 a         1184 c         1213 g          437 t
ORIGIN      
        1 atgcggggtc cggcggcatc gtgagcctcc cgccgcgcga agacaccatc gtcgccatcg
       61 ccacgccccc cggccgcggc ggcgtcggcg ccgtgcggct ctccggagag ggggccgcgt
      121 cgatcgcgcg ccggctgttc cgcccgtcgg gagcgccgac cggcccggat cgtttcgaac
      181 cgggacgggc cctgcccggg acgttcgtgc tcgccgacgg cgctccgatc gatcgcggct
      241 atctcgtcct cttcgccgcc ccgcgctcgt tcaccggcga ggaggtcgcc gagctgtgga
      301 cgcacggcag cccgccggtg ctgcgcgcgc tggtcgacgc ggcggtgtcg tgcggcgccc
      361 gtccggccac ccccggagaa ttcagctacc gcgcgctgct caaccgccgg atcgacgcca
      421 cgcaggcgga ggcgatccgc gatctcgtcg aggcgcgcac cctgcaccag gcgcgggtcg
      481 cgcacgccca ggaggccggc gccctgtccc gcgccctccg accgctcagc gcgcgtctcg
      541 cggacgtcgc ggcgcgggtc gaggcggcga tcgagttctc cgaggagccg gaagtcgagc
      601 gggccgacgc ggaaggcggg gccgcggaaa tcctgaggtc gacgctcgcc tcgctgcagg
      661 acctcctggt cgggttcgat cgcggccgcc gactgcgcga gggggcgtcg gtcgtcctcg
      721 ccggactgcc gaacgccggg aaatcgagcc tcttcaatcg cctcgtcggc agcgcgcggg
      781 ccatcgtcac cccggtcgcc gggacgacgc gcgacgtcgt ggaggagacg atcgagatcg
      841 gcggcgtccc cgtgaccctc gccgacaccg ccggtctcca cgacgcggcc aacgaggcgg
      901 accgggaggc ggtgcagcgg gcgcgcgcgg ccgccgccgg cgccgacatc gtcctcgtcg
      961 tgctcgactg gagccgcccg tggcgcgacg ccgaccgtga gacgatcgag gcggcgggag
     1021 cggatcgggc cctcgtcgtc ctcaacaagg tcgatctccc ctgccgcgtc ggtctcgacc
     1081 ggatcctctc cctccgcaag cggaacgcgg cgctcgaggt gtcggccggg accggcgagg
     1141 gggtcgagga ccttcgccgg ctcctcctcg agcggatcgc tccgctcggc gcgcggcagc
     1201 cggacgaaat cttcctgacg agcgtgcggc agaaggacct cgtcgcgcgc gctgcggccg
     1261 cgatcgagcg ggcccttcag gccgggcgcg agcggcgcgg cctggagtgc gtcgccgtcg
     1321 atctgcgcga cgcgctcgac cggctcggcg agctgagcgg cgagatccac ctggacgatc
     1381 tctacgaccg gctgttctcc accttctgca tcggcaaatg atcgcgtccc gcgccgatgt
     1441 cgtggtggta ggggccggcc acgcgggcct cgaggcggcc ctggccgcgg cccggatggg
     1501 atgctccacc ctgctcgtca cgctcgcgcg cgaggcgacc ggccggatgt cgtgcaaccc
     1561 ggcgatcggc gggctcgcga aggggcacct ggtgcgtgaa atcgacgcgc tggggggcga
     1621 gatggggcgg gcgatcgacg ccaccggcat ccagtttcgt ctcctcaacg ccagccgcgg
     1681 gccggcggtg caggcgccgc gcgcgcaggc cgacaaggaa gcgtacgccc ggcgcatgcg
     1741 cgagaccgtc gagcgtaccg aacgcctggg gctcgtcgag gacgaggtga tcgacctcgc
     1801 ggtcgagggg ggtcgcatca ccggcgtgat cctgcgccgc gcgggccgcg tcgacgccgc
     1861 cgcggtcgtg gtcacgagcg ggacgttcct gcgcggcctc atccacatcg gatcgtcgca
     1921 gcgcgccggc ggccgaatcc acgaggcggc cgcggaaggc ctgtcggcgg ctctccagag
     1981 gttggggctg ccgctcgggc gcctcaagac cggcacgccc ccgcgcatcc tgaaggacag
     2041 cgtcgatcgc acgcgtctcg agcggcagcc gggggacaag gagccggtgc cgttctcctt
     2101 cgagaccggg cggatcgacc ggccgcaagt ggactgccac atcacctgga cgagcccgga
     2161 gacgcaccgg ctcatccgcg ccaacctcga tcgctcgccg ctctacaccg gcgtcatccg
     2221 ctcggtcggc ccgcgctact gcccgtcgat cgaggacaag gtggtgcgct tcgccgatcg
     2281 cgagcggcac cagatcttcg tggagccgga ggggattgat catccgtggc tctacctcaa
     2341 cgggctgtcg acgagcctgc ccgaggaagt gcagcagccg ctggtccgca cccttcccgg
     2401 cctcgaggag gcggtggtcg cgcgccccgg ctacgcggtg gagtacgact tcgtcccgcc
     2461 gaccgcgtgc cggcacacgc tcgagacgaa gacggtcgcg ggactcttcc tcgccggcca
     2521 gatcaacggg acgaccggtt acgaggaggc ggcggcgctc ggccttctcg ccggcgtgaa
     2581 cgccgggctc caggcacagg gacggccgcc gttcgtgctg tcgcgcggcg cggcgtacgc
     2641 gggggtgatg gtggacgatc tcgtgacgcg cggcacgagc gagccgtacc ggatgttcac
     2701 ctcgcgcgcc gagtgccggc tgctgctcga catcgacacg gccgacttga ggctcacccc
     2761 gtacggccgt gatctggggc tcatcggcgc cgggcgtttc gctcgcttcg aggagcggcg
     2821 ccggcgcatc gccgcctggt ccgccgtcct cgagacgcgg gcgatcctcc cgaatgccgc
     2881 gaccaacgaa cgggcgcggc gcgatctggg aatcgtcctc aacgagccga ccacggcggc
     2941 gcgcctgctg cgccgcagcg acgtcacggc cgaagggatc gagcgcttcc tggccgagga
     3001 tctccctcga gggttgacgg tgcgggagcg gaacacggtg gcgaaccgcc tgcgctacgg
     3061 cggctacatc gagcgccagg agcggcacgt cgatcgcctg cggcgcgagg aggggaggcg
     3121 catcccggac gacttcgact acagggcgat cgccggcctt tcgcgcgagg tggtggagaa
     3181 gctgacgatt gcgcgtccag gaacgctgcg ggaggccggc ctcatctccg gcgtgacccc
     3241 ggcggccctc gtcctggtga acgtcgcg
//
LOCUS       DASRIQ010000219         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3483847, whole genome shotgun sequence.
ACCESSION   DASRIQ010000219 DASRIQ010000000
VERSION     DASRIQ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3483847"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFD06_05455"
     CDS             <1..1180
                     /locus_tag="VFD06_05455"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZN03014.1"
                     /translation="IGKQQAAYEDLCRLLMPPASAAYYDRYPSRRRKDAQWNREIKKK
                     AQEVARYVLPVATFAYLYHTINGITLLRYWRTCRQYDAPLEQRLLVGRMVEELLRLDP
                     AYKAVLEEPMADDELPEQRAARDLADGDGDRVRRFRQEFDAQLSGRTSVLIDYPAKNE
                     ETMAQAVREVLGLPRAGLADDAAIEMVLDPAQNRYLGEALNLTTLSKLSRAMAHAHYT
                     FRKKMSHAGDSQNQRHRTTPASRPILELQIDDEPDFETPTLVGTDEACARRYREAMET
                     AWDGVRRLRALGTPAEYALYLLPNAVSIRFTESASLLDLRHKHAMRLCYNAQEEIWRA
                     SLDEALQIRAVHPRIGRWLLPPCTLRQGAGLTPVCPEGRRYCGERVWTYDIEEYRRVI
                     "
     gene            1184..1777
                     /gene="trmB"
                     /locus_tag="VFD06_05460"
     CDS             1184..1777
                     /gene="trmB"
                     /locus_tag="VFD06_05460"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HZN03015.1"
                     /translation="MGDLEILPGQVDGPLEIESIFGRRAPFEVEVGIGKGRFLLAEAE
                     RRPDTDFLGLEWSLKHLRLAKERAAKRGLANLRLHRADARHVLADLLRDGSAVRIHVY
                     CPDPWPKKRHHKRRLFDAETTRHLERILARGGYLHVSTDVADYFEAIVTAVAAHTGLI
                     PAEDPEFPDAPAGGNTNYEAKYLAAGRRIHRATWRRE"
     gene            1770..2726
                     /locus_tag="VFD06_05465"
     CDS             1770..2726
                     /locus_tag="VFD06_05465"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HZN03016.1"
                     /translation="MNKPARGPAAALLAGVFLTGCASIQKMAVNRLGDFLAEGGSVYE
                     SDDDPDLVADALPFGLKLMESLLESSPDHPGLLRAACKGFTVYTYAYVDAEAERMEES
                     DLDAARAMRARARRLYLRAWGYGERGLALAAKRAPAILADPRAAVAAMKEKDVPLLYW
                     SAAALGLAISDSRGDAEMIARLPAVEALLDRARALDPDWGEGALDEFDIVLAGTRPGA
                     PLDEERLRADYERALRLSEGKRASLFVGYAEQSAVKRQDPAEFRDLLDRALAIDPDAH
                     TSIRLANLVAQRRARWLLDHVDSLFLDPDAGAAAAGAKPEKP"
     gene            2723..3802
                     /gene="dctP"
                     /locus_tag="VFD06_05470"
     CDS             2723..3802
                     /gene="dctP"
                     /locus_tag="VFD06_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZN03017.1"
                     /translation="MSLPTVTSAARRPALVSAGLVVIVALSAFGPAALPWPAAAAPAG
                     APVTIKLATVAPEGSPWHEVLKQMGQDWERTSSGKVKLRLYPGGVLGDEAALVTKLRI
                     GQLHAAALSAVGLYRIDRGVACLGVPMMFDTYEELDRVRDKLAPRLEKAFEAQGFVVL
                     NWGDAGWIRFFSKEPARTPDDIRRMKLFIAADDAETLELYKALGVKPVPLAETDILAS
                     LQTGLITAFDVPPLLALLNQWFGVAPHMLDVRWAPLVGATLISRKAFMTLPADVRPQL
                     LEAGRQAGVKFRGDMRRLSEEAIPAMEKRGLKVHRPDEATLALWRKEAEGAWPRLRGS
                     MVPADLFDEAKRLRDEARSGKPSPP"
     gene            3799..>4837
                     /locus_tag="VFD06_05475"
     CDS             3799..>4837
                     /locus_tag="VFD06_05475"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZN03018.1"
                     /translation="MSAGAAVDRAARRVEEALAILALGIMVLLPMLEIVGRRLVGVGV
                     PGSVLIVQNLTLWVAFLGAVLAARRGQLLALSSALFLPERFRPAARAAVAILATAVVG
                     WIARASLDLLAAEREAGGDLVPGVPRWVVVAILPLGSLAVAAHLIRGASARWPGRLFA
                     AAGLLLPFLLEQAASHGEGAATAGILVLIAAAACGMPLFAVIGGAALLLFWGQGSPVA
                     AVPAETHRMVTFPGLPAVPLFTLAGYILAAGGASGRLLKVFSSLFGWLPGGTAIVTTV
                     LFAFFTSFTGASGVTILALGGLLLPVLLADNYPEDFAIGLLTVSGSIGLLFPPSLPVI
                     LYGVQSNQSIAD"
BASE COUNT          641 a         1785 c         1703 g          708 t
ORIGIN      
        1 gatcgggaag cagcaggcag cgtacgagga cctgtgccgc ctgctcatgc ccccggcgag
       61 cgccgcgtac tacgaccgct acccctcccg ccgccgcaag gacgcgcagt ggaatcgcga
      121 aatcaagaag aaggcgcagg aggtggcgcg ctacgtcctg ccggtcgcca ccttcgcgta
      181 cctgtaccac accatcaacg gcatcacgct cctgcgctac tggcggacct gtcgccagta
      241 cgacgcgccg ctcgagcagc gcctgctcgt cggccggatg gtcgaggagc tgctgcgcct
      301 cgaccccgcc tacaaggcgg tcctcgagga gccgatggcc gacgacgagc tccccgagca
      361 gcgggcggcg cgggacctcg ccgacggaga cggcgatcgc gtgcgccgct tccgccagga
      421 gttcgacgct cagctctctg gccgcacctc ggtcctgatc gactacccgg cgaagaacga
      481 agagacgatg gcgcaggcgg tgcgcgaggt gctgggcctg ccgcgcgcgg gcctggcgga
      541 cgacgccgcc atcgagatgg tcctcgaccc ggcgcagaac cgctacctcg gcgaggcgct
      601 gaacctcacg accctgagca agctgtcgcg cgccatggcg cacgcccact acaccttccg
      661 caagaagatg agccacgccg gggactcgca gaaccagcgg caccgcacca ccccggcgtc
      721 gcgcccgatc ctcgagcttc agattgacga cgagccggac ttcgagaccc cgacgctggt
      781 cggcaccgac gaggcctgcg cccggcgcta tcgcgaggcg atggagacgg cctgggacgg
      841 cgtgcggcgc ctccgggcgc tcgggacgcc ggcggagtac gcgctgtacc tcctgccgaa
      901 cgccgtgtcg atccgcttca ccgagtcggc gagcctcctc gatctccggc acaagcacgc
      961 catgcgcctc tgctacaacg cccaggagga gatctggcgc gcctcgctcg acgaggcgct
     1021 gcagatccgc gcggtgcacc cgcgcatcgg ccgctggctc ctccccccct gcacgctgcg
     1081 ccagggtgcg gggctgactc cggtctgccc ggaggggcgg cgctactgcg gcgaacgggt
     1141 ctggacctac gacatcgagg agtatcgccg ggtcatctga tccatgggcg atctcgagat
     1201 ccttcccgga caggtcgacg gcccgctgga gatcgaatcg atcttcgggc gcagggcccc
     1261 cttcgaggtc gaggtcggca tcggcaaggg gcggttcctc ctggcggagg cggagcggcg
     1321 tccggacacc gatttcctcg ggctggagtg gtcgctcaaa cacctgcgcc tcgcgaagga
     1381 gcgggcggcg aagcgtgggc tcgcgaatct gcgcctgcac cgcgccgacg cgcggcacgt
     1441 cctcgccgat ctcctgcgcg acggaagcgc cgtgcgcatc cacgtctact gccccgatcc
     1501 gtggccgaag aagcggcacc acaagcggcg gctgttcgac gcggagacca cccgccacct
     1561 tgagcgcatc ctcgcccgcg gcggctacct gcacgtatcc accgacgtcg ccgactactt
     1621 cgaggcgatc gtcaccgccg tcgccgcgca caccgggctc atccctgcgg aggacccgga
     1681 gttcccggac gcccccgccg gcgggaacac caactacgag gccaagtacc tggccgccgg
     1741 acgccgcatc caccgggcga cgtggcggcg tgaataagcc ggcacggggc cccgcggcgg
     1801 cgcttctcgc gggcgtcttc ctgaccggct gcgcctcgat ccagaagatg gcggtgaacc
     1861 ggctggggga cttcctcgcc gagggggggt cggtgtacga aagcgacgac gaccccgatc
     1921 tcgtcgccga cgcgctgccg ttcggcctca agctgatgga gagcctgctc gagtcctccc
     1981 ccgatcaccc ggggctcctg cgcgccgcgt gcaaggggtt caccgtgtac acgtacgcct
     2041 acgtcgatgc ggaggcggag cggatggagg agagcgatct cgatgccgcc cgggcgatgc
     2101 gggcccgggc ccggcgactg tacctgcgcg cctggggcta tggcgagcgc ggcctggcgc
     2161 tggccgcgaa gcgcgcgccg gcgatcctcg cggatccacg ggcggcggtg gcggcgatga
     2221 aggagaagga cgtgccgctt ctctactgga gcgccgccgc gctgggcctc gccatctccg
     2281 acagccgggg ggacgcggag atgatcgcgc ggctgccggc ggtcgaagcg ctcctcgacc
     2341 gggcccgcgc cctcgatccc gactgggggg aaggggcgct cgatgagttc gacatcgtcc
     2401 tcgccgggac gcgtccgggg gcgccgctcg acgaggagcg cctgcgcgcc gactacgagc
     2461 gggcgctgcg cctgtcggag gggaagcgcg ccagcctctt cgtcggctac gcggagcagt
     2521 cggcggtgaa acggcaggac cccgcggagt tccgcgacct gctcgaccgc gccctcgcca
     2581 tcgacccgga cgcccacacc tccattcggc tggccaacct cgtggcgcag cggcgcgccc
     2641 gctggctcct cgaccacgtc gattccctct tcctcgaccc ggacgccggc gccgccgcgg
     2701 ccggagcgaa accggagaag ccgtgagcct tccgaccgtg acatccgccg cgcggcgtcc
     2761 cgcgctcgtg tccgccggtc tcgtcgtcat cgtcgccctg tccgccttcg ggccggccgc
     2821 gctgccgtgg cccgccgccg cggctcccgc cggcgctccg gtgaccatca agttggcgac
     2881 cgtggcgccc gagggatccc cctggcacga ggtcctcaag cagatgggtc aggactggga
     2941 gcgaacctcc tcggggaagg tgaagctgcg tctctatccc ggcggcgtgc tcggcgacga
     3001 ggcggcgctc gtcaccaagc tgcgcatcgg ccagctgcac gccgcggcgc tgtcggcggt
     3061 cgggctctat cgcatcgacc gcggcgtcgc ctgcctcggc gtgccgatga tgttcgacac
     3121 ctacgaggag ctcgaccggg tgcgcgacaa gctggcgccg cggctcgaga aggcgttcga
     3181 ggcgcagggg ttcgtcgtcc tcaactgggg ggacgccggc tggatccgct tcttctcgaa
     3241 ggagccggcc cgcacccccg acgacatccg ccgcatgaag ctgttcatcg ccgccgacga
     3301 cgccgagacc ctcgagctgt acaaggcgct cggggtgaag ccggtgccgc tcgccgagac
     3361 cgacatcctg gcgtcgctgc agaccgggct catcaccgcc ttcgacgtgc cgccgcttct
     3421 ggcgctgctc aaccagtggt tcggcgtcgc gccgcacatg ctcgatgtgc gctgggcccc
     3481 tctcgtcggc gccacgctca tcagccgcaa ggctttcatg acgctgccgg cggacgtccg
     3541 cccgcagctg ctcgaggccg gccgccaggc cggcgtgaag ttccgcggcg acatgcgccg
     3601 gctgagcgag gaggcgatcc ccgcgatgga gaagcgcggg ttgaaggtgc accgcccgga
     3661 cgaggcgacg ctcgcgctgt ggcgcaagga ggcggagggc gcctggccgc gcctgcgcgg
     3721 cagcatggtc ccggccgacc tgttcgacga agcgaagcgc ctgcgcgacg aggcgcgctc
     3781 cggaaagccc tccccgcctt gagcgcgggc gcggcggtcg atcgcgcggc ccgccgcgtc
     3841 gaagaggcgc tggcgatcct cgccctcggc atcatggtgc tgctgccgat gctcgagatc
     3901 gtcgggcggc gcctggtcgg cgtcggcgtc cccggctcgg tcctcatcgt ccagaacctg
     3961 accctgtggg tcgccttcct cggcgccgtg ctcgccgccc gccgcggcca gctgctggcc
     4021 ctttcctccg ccctgttcct gccggagcgg ttccgtccgg cggcccgcgc cgccgtcgcc
     4081 atcctggcga ccgcggtggt cgggtggatc gcccgcgcca gtctcgatct gctggctgcg
     4141 gagcgcgagg cgggagggga tctcgtcccg ggagtgccgc ggtgggtcgt cgtggcgatc
     4201 ctgccgctcg gatcgctcgc ggtcgccgcc cacctcatcc gcggcgcctc ggcccgctgg
     4261 ccgggacgcc tgttcgcggc ggccggcctc ctgctgccgt tccttctcga acaggcggct
     4321 tcgcacggcg agggggcggc gaccgcgggc attctggtgc tgatcgcggc cgcagcctgt
     4381 ggcatgccgc tcttcgccgt catcggcggc gcggccctgc tcctcttctg ggggcaggga
     4441 tcgccggtcg cggcggtgcc ggccgagacg caccggatgg tgacgttccc ggggcttccc
     4501 gccgtgccgc tcttcaccct cgccggctac atcctggcgg cgggcggggc gagcgggcgc
     4561 ctcctgaaag tgttttcgtc cctcttcggc tggctgcccg gagggacggc gatcgtcacg
     4621 acggtcctgt tcgccttctt cacgtcattc acgggcgcct cgggggtcac catcctggcg
     4681 ctcggcggac tcctgctccc ggtgctgctg gccgacaact acccggagga tttcgccatc
     4741 gggctcctca ccgtctcggg atcgatcggg ctcctgttcc cgccgagcct gccggtgatc
     4801 ctgtacggtg tgcagtcgaa ccagtcgatc gcggatc
//
LOCUS       DASRIQ010000220         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1148105, whole genome shotgun sequence.
ACCESSION   DASRIQ010000220 DASRIQ010000000
VERSION     DASRIQ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148105"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1606)
                     /locus_tag="VFD06_05480"
     CDS             complement(206..1606)
                     /locus_tag="VFD06_05480"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN03019.1"
                     /translation="MSRKSSILVLHNDPSFAKCLDEALASAGHKVTAVDEEKAAIERG
                     RSRAYDMALVDLDRPGAGLGFVEEMKGACPELPVAIAGRALDVDVVIGAMRRGVWEVL
                     RKPCASEDLLRLAERAARIKEMGSERRRTAEDLSAERARNAELRRSLQGEHAFSAILG
                     RSAKMAQILALLKEVCATDSTVLIMGESGTGKGLVARILHYNSHRAAGPFVEANCAVY
                     SEGVLHSELFGHEKGAFTGALKQKKGRFELADGGTVFLDEIGDLTPSTQLMLLRFLQE
                     RRFERVGGEETLHVDARVIAATHKNLKEGMERGSFRHDLFYRLNVIPLQIPPLRDRPE
                     DVPVLGAAFLDRFSRKIGKPMRGFSDEALEILGRHGWPGNVRELENVIERTMVLAKGD
                     RIEASDLPGALRDGNDPTVDARLSLLEHERLYILKTLAECNWNKKLAASVLGINRSSL
                     YSKLKKHVIGRGPSLN"
     gene            1860..>2564
                     /locus_tag="VFD06_05485"
     CDS             1860..>2564
                     /locus_tag="VFD06_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03020.1"
                     /translation="MRLARLAIGLPLALLTGGSAARAPLDAGLQAMHVGDYRSAIVEF
                     RRAADSDPGSPDPVYLLLFSRWSQRAFEEGENTKADPVFDTAYDDLVRVAAARLAESP
                     KDADALAALGGGQVLRAQVEAVRGNYFRAGREARQGKKALERALAISPSLETALFPMG
                     ALNYYADRVPLLIKGIRPLLAIPGGDVDLGLRQIKEVADGNGRFQVEARLLFGILCAD
                     RYQKRYREAVRHLERAV"
BASE COUNT          362 a          892 c          918 g          392 t
ORIGIN      
        1 cgccaccagg gcgccgagga tcgccagggc cagcgtggag gaggtgagga cgaggtggtg
       61 cccggggagg atcgcgagca ggacgaggac ggcccccgcc acggtggcgg cgatgtagag
      121 gagaacgccc cgatcgagct cgcgcatgac ggctttccgg gcgccccggc gcggggccgg
      181 agcgccgcgc ctcagcccgg cctcctcagt tcagcgacgg cccgcggccg atgacgtgct
      241 tcttgagctt gctgtacagg ctgctccggt tgatcccgag gaccgaggcg gcgagcttct
      301 tgttccagtt gcactccgcc agggtcttga ggatgtagag gcgctcgtgc tcgaggagcg
      361 acagccgggc gtcgacggtc gggtcgttcc cgtcccgcag cgcccccggc aggtcgctcg
      421 cctcgatccg gtcccccttg gcgagcacca tggtccgctc gatgacgttc tccagctcgc
      481 gaacgttccc gggccagccg tgccggccga ggatctcgag cgcctcgtcg gagaagccgc
      541 gcatcggctt gccgatcttg cgagagaagc ggtcgaggaa cgcggcgccg agcaccggca
      601 cgtcctcggg ccggtcccgc agcgggggga tctgcagcgg gatgacgttg aggcggtaga
      661 agaggtcgtg ccggaaggag ccccgctcca tcccctcctt caggttcttg tgggtcgcgg
      721 cgatgacgcg cgcgtccacg tgcagcgtct cctcgccgcc cacccgctcg aagcgccgct
      781 cctgcaggaa gcgcagcagc atcagctggg tcgagggggt gagatcgccg atctcgtcga
      841 ggaagaccgt gccgccgtcc gccagctcga accgcccctt cttctgcttg agcgctccgg
      901 tgaaggcccc cttctcgtgg ccgaagagct ccgagtgcag caccccttcg gagtagacgg
      961 cgcagttcgc ctcgacgaac ggtccggcgg cgcgatgcga gttgtagtgg aggatgcgcg
     1021 ccaccagccc tttgccggtg ccgctctccc ccatgatgag cacggtcgag tcggtggcgc
     1081 agacttcctt caggagcgcc aggatctgcg ccatcttcgc gctccggccc aggatggcgg
     1141 agaaggcgtg ctccccctgg agcgagcgcc gcagctcggc gttgcgcgcc cgctcggcgc
     1201 tgaggtcctc ggcggtgcgc cgccgctccg atcccatctc cttgatgcgg gccgcgcgct
     1261 cggccaggcg gagcaggtcc tccgaggcgc acggcttgcg caacacttcc cagacgccgc
     1321 ggcgcatcgc gccgatgacc acgtcgacgt cgagggcgcg cccggcgatc gccaccggca
     1381 gctccgggca ggcccccttc atctcctcca cgaatcccag cccggcgccg ggacggtcga
     1441 gatcgacgag ggccatgtcg taggcgcggc tgcggcctcg ctcgatcgcc gccttctcct
     1501 cgtctaccgc cgtgaccttg tgcccggcgg aggcgagcgc ttcgtcgagg cacttcgcga
     1561 acgacggatc gttgtgcagg accaggatgc tggacttgcg gctcatgcgg ccccccttcg
     1621 tgtccagaag atcggcaaga accgttccac gaagccggga aggacggtcg cgaaacccgc
     1681 gtcgggagcg gcgtttacgc gatcacgatc gacggatcgg cgccggtgtc gtgaatcccg
     1741 acaaaagtgg caggaccggg tgacgaggag tgtcaggaga ccggcgccgg gcggcgcgtg
     1801 gaccggcgcc cgggggtgaa ggtggcgcgg cggcgcggcg atgctagatt gctggggtca
     1861 tgcgcctcgc acgtcttgcg atcggactcc ctctcgcgct cctgacgggc gggtcggcgg
     1921 cccgagcgcc tctcgacgcg gggctccagg cgatgcacgt cggcgactat cgcagcgcca
     1981 tcgtcgagtt ccgccgcgcc gccgactccg atcccgggtc tcccgatccg gtctatctcc
     2041 tgctcttcag ccgctggtcg caacgcgcct tcgaggaggg ggagaacacc aaggccgatc
     2101 cggtcttcga caccgcctac gacgacctgg tgcgggtggc cgcggcgcgc cttgccgagt
     2161 ccccgaagga tgccgacgcc ctcgcggccc tcggcggagg acaggtgctg cgcgcccagg
     2221 tcgaggcggt gcggggcaac tacttccggg cgggacgcga ggcgcgccag gggaagaagg
     2281 cgctcgagcg ggcgctcgcg atctccccga gcctcgagac cgcgctcttc ccgatggggg
     2341 cgctcaatta ctacgccgac cgcgtgccgc tcctcatcaa ggggattcgc ccgctgttgg
     2401 ccatccccgg gggggatgtc gatctcggtc tgcgtcagat caaggaagtg gccgacggca
     2461 acggccggtt ccaggtcgag gcgcggcttc tcttcggcat cctgtgcgcc gatcgctacc
     2521 agaagcgcta ccgcgaggcg gtgcggcacc tcgagcgggc ggtc
//
LOCUS       DASRIQ010000221         4878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1908944, whole genome shotgun sequence.
ACCESSION   DASRIQ010000221 DASRIQ010000000
VERSION     DASRIQ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908944"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFD06_05490"
     CDS             complement(<1..661)
                     /locus_tag="VFD06_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZN03021.1"
                     /translation="MSKSSPFSDKARTINDFQSIRISLASPEKIRSWSYGEVTKPETI
                     NYRTFKPERDGLFCAKIFGPVTDWECLCGKYKRMKHRGVICDKCGVEVTQAKVRRERL
                     GHITLATPVSHVWFFKGLPSRIGHLLDISLRDLERVLYFEAYVVIEPGDTDLKQNQLL
                     TEEQFRKAREDYGIKFRAQMGAEAIKELLKRVSVEKMAVELREKMRGENSVQKKLKYA
                     KR"
     gene            complement(688..>4878)
                     /gene="rpoB"
                     /locus_tag="VFD06_05495"
     CDS             complement(688..>4878)
                     /gene="rpoB"
                     /locus_tag="VFD06_05495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZN03022.1"
                     /translation="SYERFLQIYTAPADREDIGLQAVFKSIFPISDFRQTCSLEFVEY
                     TIGNWECKCGKLEGLEHLRIACVKCGKKSISHNPKNPRLTCPDCGWSNENRVVHCEIC
                     GDPVSLKYKYDVAECQERGMHFTVPLKVKIRLVVYEKDSASGQKEIRDIKEQEVYFGE
                     IPLLTENGTFIINGTERVIVSQLHRSPGVFFQSDDGKKTWMAKVIPYRGSWLEFEFDD
                     KDLLHVRIDRKRKFLGSVVLRAIGHKTDTDILRTFYRTETIEVEDGKLFWRTGPGLAG
                     HRAKGEVTDKKGKEVLIPKGKKITAHMLQALEKAGVERVPVDAEELGGALAMSDIVDI
                     KSGEVLLEANEPLTGVILDQTVGRGLPSIEIFFPQDSEIGTVLPDTVRKDTVKAPDEA
                     LVEIYRRLRPGDPPTLDGSRTLFNGMFFDPNRYDLSRVGRLKMNTKLGTDRPLDQRVL
                     TPDDFVRVLKHLLGLRRNPSGVDDIDHLGNRRVRAVGELLENQFRIGLVRMERAIKEK
                     MSVYQEMTTAMPHDLINAKPVMAAIQEFFGSSQLSQFMDQTNPLSEITHKRRLSALGP
                     GGLSRERAGFEVRDVHPTHYGRICPIETPEGPNIGLISSLSCYARINEFGFIESPYRK
                     VQGGRVRDYYRVASKGESEFELGQVIEKDDFEREMNKLKRRKEKVPDAEPHPFYLTAW
                     EEDKAIIAQANVRLDDQGLMVEDRVTARVAGEFKFVERDEVHYIDVSPKQLVSVAASL
                     IPFLENDDANRALMGSNMQRQAVPLVRAEAPFVGTGMEKITAQDSGAVVLCQREGSVD
                     YVDSQRIIVRVSGEGYGLKETDDFGADIYNLTKFKRSNQNTCINQKPIVAAGQKVKRG
                     DVLADGPCTDGGELALGRNVLVAFMPWRGYNFEDAILVSERLVSDDYFTSIHIEEFEI
                     EARDTKLGPEEITRDIPNVSEDFLRNLDESGIIRIGAHVKAGDILVGKVTPKGETQLT
                     PEEKLLRAIFGEKSGDVRDASLTTPPGIEGTVVDVKIFCRKGVEKDGRALQIEQEQIA
                     RMEKNLNDEIRILKEENRNRMTELLLDHKLAEPLTEKRRKKELLPKGTVLTREVMAKL
                     GVDDLMRIEFEGRKGEAEEIREIEERTERKVQILRRLHEEKIQQLRKGDELSPGVIKM
                     VKCFVAMKRKLSVGDKMAGRHGNKGVIARILPPEDMPYLPDGTPVEIVLNPLGVPSRM
                     NVGQILETHLGWAAKALGLHFATPVFDGASEREIKDYLKQADLPSTGKTHLHDGRTGE
                     RFDQKVTVGYIYMLKLSHLVDDKIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWA
                     LEAYGAANILQELLTAKSDDVYGRTKIYEALVKGETTVEPGLPESFNVLVRELQSLCL
                     DVELRAGDGAGPAGGDVR"
BASE COUNT          691 a         1618 c         1613 g          956 t
ORIGIN      
        1 ggcgcttggc gtacttcagc ttcttctgga cggagttctc accgcgcatc ttctcgcgca
       61 gctcgaccgc catcttctcg acgctgaccc gcttcagcag ctccttgatc gcttccgcgc
      121 ccatctgtgc gcggaacttg atcccgtagt cttcgcgcgc cttgcggaac tgctcctcgg
      181 tgaggagctg gttctgcttg aggtcggtgt cgccgggctc gatgacgacg taggcctcga
      241 agtagaggac gcgctcgaga tcgcgcagcg agatgtcgag caggtggccg atgcggctcg
      301 gcagcccctt gaagaaccac acgtggctca ccggcgtggc gagcgtgatg tggccgaggc
      361 gctcgcgccg gaccttggcc tgcgtgacct cgacgccgca cttgtcgcag atcacgccgc
      421 ggtgcttcat gcgcttgtac ttgccgcaca ggcactccca gtcggtgacc gggccgaaga
      481 tcttggcgca gaagagcccg tcgcgctccg gcttgaaggt gcggtagttg atcgtctccg
      541 gcttcgtcac ctcgccgtac gaccacgagc ggatcttctc gggcgaggcg agtgaaatcc
      601 ggatcgactg gaagtcattg atcgtccgcg ccttgtccga gaacggactg ctcttgctca
      661 tggggcggaa gccttcggtg tagggcctca tcgaacgtca cctcccgcgg gacccgcgcc
      721 gtcgccggcg cgcagctcga cgtccaggca caggctctgc agctcccgca ccagcacgtt
      781 gaacgactcg gggagccccg gctccacggt cgtctctccc ttgaccagcg cctcgtagat
      841 cttggtgcgg ccgtacacgt cgtccgactt cgcggtcagc agctcctgca ggatgttggc
      901 ggcgccgtag gcctcgagcg cccagacctc catctctccg aagcgctggc cgccgaactg
      961 cgcctttccg ccgagcggct gctgggtgat cagcgagtac ggcccgatcg agcgggcgtg
     1021 gatcttgtcg tcgaccaggt gcgacagctt cagcatgtag atgtatccga ccgtgacctt
     1081 ctgatcgaag cgctcgccgg tgcggccgtc gtgcaggtgg gtcttgccgg tggacggcag
     1141 gtccgcctgc ttgaggtagt ccttgatctc acgctcgctg gcgccgtcga acaccggggt
     1201 ggcgaagtgc agccccagcg ccttggcggc ccatccgagg tgggtctcga ggatctgccc
     1261 gacgttcatc cggctcggaa cgccgagcgg gttgagcacg atctcgaccg gcgtcccatc
     1321 cggcaggtac ggcatgtcct ccggtggcag gatgcgggcg atgacgccct tgttgccgtg
     1381 ccgccccgcc atcttgtcgc cgaccgacag cttgcgcttc atggcgacga agcacttgac
     1441 catcttgatg acgcccggcg acagctcgtc ccccttgcgc agctgctgga tcttctcctc
     1501 gtgcaggcgc ctgaggatct gtactttgcg ctcggtgcgc tcctcgatct cgcggatctc
     1561 ctccgcctcc cccttgcgcc cctcgaactc gatgcgcatc aggtcgtcga cgcccagctt
     1621 cgccatcacc tcgcgcgtca ggaccgtccc cttcggcagc agctccttct tgcggcgctt
     1681 ctcggtgagc ggctccgcca gcttgtggtc gaggaggagc tccgtcatcc ggttgcggtt
     1741 ctcttccttg aggatgcgga tctcgtcgtt gaggttcttc tccatccggg cgatctgctc
     1801 ctgctcgatc tgcagcgccc ggccgtcctt ctcgacccct ttgcggcaga agatcttgac
     1861 gtcgacgacc gtcccttcga tgcccggcgg ggtggtgagc gacgcgtcgc gcacgtcccc
     1921 cgacttctcg ccgaagatcg cccgcagcag cttctcctcg ggggtgagct gggtctcccc
     1981 cttcggggtg accttgccga ccaggatgtc gccggccttg acgtgggcgc cgatgcggat
     2041 gatcccggac tcgtccaggt tccgcaggaa atcctccgag acgttcggga tgtcgcgggt
     2101 gatctcctcc ggtcccagct tcgtgtcgcg cgcctcgatc tcgaactcct cgatgtggat
     2161 cgaggtgaag tagtcgtcgg agacgaggcg ctcggagacg aggatggcgt cctcgaagtt
     2221 gtagccgcgc cacggcatga aggcgacgag cacgttccgc ccgagcgcca actcgccgcc
     2281 atcggtgcac ggcccgtcgg ccagcacgtc gccgcgcttg accttctggc ccgccgcgac
     2341 gatcggcttc tggttgatgc aggtgttctg gttcgagcgc ttgaacttcg tcaggttgta
     2401 gatgtcggct ccgaagtcgt cggtttcctt gagcccgtat ccttcgcctg agacgcgcac
     2461 gatgatgcgc tgggagtcga cgtagtcgac cgacccctcg cgctggcaca gcacgaccgc
     2521 cccggagtcc tgggcggtga tcttctccat gccggtgccg acgaacggcg cctcggcgcg
     2581 gaccagcggc accgcctgac gctgcatgtt cgagcccatc agggcgcggt tcgcgtcgtc
     2641 gttctcgagg aacgggatga gcgacgcggc gaccgacacc agctgcttgg gcgacacgtc
     2701 gatgtaatgc acctcgtcgc gctcgacgaa cttgaactcg ccggcgacgc gggcggtgac
     2761 gcggtcctcc accatgagcc cctggtcgtc gagtcggacg ttggcctggg cgatgatggc
     2821 cttgtcctcc tcccaggcgg tcaggtagaa ggggtgtggc tcggcgtccg ggaccttctc
     2881 cttgcgccgc ttcagcttgt tcatctcccg ctcgaagtcg tccttctcga tcacctggcc
     2941 gagctcgaac tcggattctc ccttgctcgc gacgcggtag tagtcgcgga cccgcccgcc
     3001 ctgcaccttg cggtacggcg actcgatgaa gccgaattcg ttgatgcgcg cgtagcacga
     3061 cagggacgag atgaggccga tgttcggccc ctccggcgtc tcgatcgggc agatgcggcc
     3121 gtagtgggtc gggtggacgt cgcgcacctc gaagccggcc cgctcgcggg agagcccgcc
     3181 cggcccgagg gccgacaggc gccgcttgtg ggtgatctcc gagagcgggt tcgtctggtc
     3241 catgaactgc gacagctgcg acgagccgaa gaactcctgg atggcggcca tgaccggctt
     3301 ggcgttgatc aggtcgtgcg gcatcgccgt ggtcatttcc tggtagaccg acatcttctc
     3361 cttgatcgcc cgctccatcc gcaccaggcc gatgcggaac tggttctcga ggagctcgcc
     3421 caccgcgcgg acgcggcggt tgccgaggtg gtcgatgtcg tccacgccgg acgggttgcg
     3481 ccgcagcccc agcaggtgct tcaggacgcg gacgaagtcg tccggggtca ggacccgctg
     3541 gtcgagcggc cggtcggtcc ccagcttggt gttcatcttc aggcggccga cgcggctcag
     3601 gtcgtagcgg ttcggatcga agaacatgcc gttgaacagc gtgcgggagc cgtcgagggt
     3661 cggaggatcg cccgggcgca ggcggcggta gatctcgacg agcgcctcgt cgggggcctt
     3721 caccgtgtcc ttgcggacgg tgtcgggcag gaccgtgccg atctcggagt cctgcgggaa
     3781 gaagatctcg atcgacggca ggccgcggcc gacggtctgg tcgaggatga cgccggtgag
     3841 cggctcgttc gcctcgagca gcacctcgcc cgacttgatg tcgacgatgt cgctcatcgc
     3901 cagcgcgccg cccagctcct cggcgtccac cggcacgcgc tcgaccccgg ccttctcgag
     3961 ggcctgcagc atgtgggcgg tgatcttctt ccccttcggg atgaggacct ccttcccctt
     4021 cttgtccgtc acctcgcctt tggcgcggtg gccggcgagg ccggggccgg tgcgccagaa
     4081 gagcttcccg tcctcgacct cgatcgtctc ggtgcggtag aaggtacgca ggatgtcggt
     4141 gtcggtcttg tggccgatgg cgcgcagcac gaccgagccg aggaacttgc gcttgcggtc
     4201 gatgcggacg tgcagcaggt ccttgtcgtc gaactcgaac tccagccacg agccgcggta
     4261 cgggatgacc ttcgccatcc aggtcttctt gccgtcgtcg ctctggaaga agacgcccgg
     4321 ggagcggtgc agctgcgaca cgatgacccg ctcggtcccg ttgatgatga aggtgccgtt
     4381 ctccgtgagg agcgggatct cgccgaagta gacctcctgc tccttgatgt cgcggatctc
     4441 cttctgcccg gaggcggagt ccttctcgta gacgaccagg cggatcttga ccttgagcgg
     4501 cacggtgaag tgcatgccgc gctcctggca ctcggcgacg tcgtacttgt acttcaggct
     4561 gacggggtcg ccgcagatct cgcagtgcac gacgcggttc tcgttcgacc agccgcagtc
     4621 ggggcaggtc aggcgcgggt tcttcgggtt gtgcgagatg gacttcttgc cgcacttcac
     4681 gcaggcgatg cgcaggtgct cgagcccctc cagcttgccg cacttgcact cccagttccc
     4741 gatggtgtac tcgacgaact ccagcgagca agtctgccgg aagtcggaga tcgggaagat
     4801 cgacttgaac accgcctgca gaccgatgtc ctcgcggtcc gcgggggcgg tgtagatctg
     4861 caggaagcgc tcgtagga
//
LOCUS       DASRIQ010000222         6475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_92950, whole genome shotgun sequence.
ACCESSION   DASRIQ010000222 DASRIQ010000000
VERSION     DASRIQ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92950"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>6475
                     /locus_tag="VFD06_05500"
     CDS             <1..>6475
                     /locus_tag="VFD06_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN03023.1"
                     /translation="TVPTPSGGLAIAAAQLRLALAWIDLPSPENSGGPLINDLDLRLE
                     SPGPDSCLAAGDTRPDGAVCPAGSASDNVYYDGNDYASVNDDPALSQWSRPRAASPEV
                     HDTRNPVEAIHLTADPDNDRSFADSAIYAGRWRVTVKRGLGGATPGSITIVTGPDPDE
                     DDNNNGRLDAGEDENGNGLLDLPGQAYALIVSGPVFAAEPPPAAGPQTFPASRLSLDR
                     TAYDCGAGAVASILDTTPAASPALSSSATTFTVLGANGSVLDTETGILFSAAGSPGAT
                     TSAAVPVRLAGPALGGNGILEADTGQRIRVRYAPAGQTAVEASAVVRCSPDLIRASFS
                     TQGGRSIGDQVSIQGGCDGDAFPDAGEVVSYGVALQNRSREAQFADVVATLTPSGPGA
                     GAVRVLDSPRALGLLPDGGSNAAFFQVYVDPAVVQALPVASRVVTMRLDLDSSERGVR
                     LGRQTYTFTHALNADRDERRYSTDAITGAREVRDLNRNGVIDRAGETDPALGFVLPAE
                     DATFSSLFSGSGAPAGHFTNELGEDLNLSGSLEFAERDFIPNAAVDRGILNSNNPADP
                     AHRAPWSFDSNNGGWLSLRHSGSKVGLGIGPLPIWEYKTNGRCGFQTAGGLNKFGVWH
                     TGDTNAATPTASSPCDNHPSPSDPSTPNTTEYLFDIAVSPLLAKVNQSADARGFPWTV
                     EFQRVGLNLNIQLFDGYAGGGVNVDNDVESDGANSLLAQRSDPYYASNSGGWPVGLFR
                     FAYDYFPGDGIDPQSLQPQRTFGPFTNPDGSATLNGDETGFTGYTTNTNPYSTSPIPE
                     APPDFLPFPRPGQALAGVCTGGGAAGSPCAPGVPGDPCVVQGGACAPQTDTVAGPVRN
                     FDATLLGYEGGFASLNNGLDAVENIVTALPGRAGNRWQIGIGFWAVESASAFTDYGVA
                     FDDVVFEWNEWHPEDESVLGVPPACSRFGGTGQPAGGACATVTADRTALYECDESIEV
                     TVHDAKCLAIGSGATAALGGACVADADCGAGGICTAARPSVEVAVVTDSDANAVVVDG
                     QAVLFPTSKRFALPAVAGTPGLYKGRVAVTTVENDAAHLYVDPANDRRFAVYYFDPLC
                     DGDRDGQAGEDGFDNADGDGIPDASDKCPLVHDPGQADADGDGRGDLCDNCPNAANAN
                     QADADADGAGDACEFDDLDGDLVPNLNDNCPDVRNLNQSDIDANGRGDLCDTLKTSGI
                     TFGGSTGMADCVAGTCARPGDAVGDACVTDEDCIRGCHNNLCTQGNSGTAGGLSCLSG
                     PNTGQACTSNTNCSVCVGGTTPGVACITNSCVSACVGGTNPGAGCANDRDCAGGGTCS
                     NIGTCVVGLCFINFVAPTPTIGQACVTHADCYADLDRDADGVIDALDNCVIAANGPLG
                     GPSNQIDSDLDGLGDACDADCGGAAVAFRCRANGAPCPAPETNQPAACANPYGLGPVC
                     GFYVTNAGGCSTRDDDLDADGVGDAFDDCPATANAAVFAGGPQRDRDRDGLGDACDPP
                     AAYDDTADGLPDDVVTFQGAIACHARPLASLTLVGIPAYVDFDGDHDQFPDTGERGRV
                     SITVRNDGPALDDAVLTLVSTDPDVACITEAQVVVPSLPAGVTMTIGSLDPGQPGFGF
                     VASNALQSQPPPVPVSRIDLQIQVAAPGILGNAAPIAVSLFADYNMPPGSQQFVLGPD
                     GLAGTADDGTVIESFDSDRDEDGNVTVRDTFLEAVAPGVFRGSCSNAPLTACVTAADC
                     PASPPGAVCQSGAYLRGIAGGSGLNRVAAVVCGGFDAYSFGPCVLDPDFPMDWHLHCA
                     PGAANCPNVESGTCVGGCSFNTPTGGQRALSPPNSLHMGAHFVPGDALAGDTTHLRAL
                     QGFMTAPLNLAFLPRPGDLDLSFFHIARLMDNNGVGPNNEHQCVDCADVQIQVDQNPD
                     PNVDAWGFWDKMAPYQNVYDHKPVAWSTFGSYYCVFTPADAGAAPPNPRGVHETICYP
                     QGAWSHCGSTIGTTPAATVDCAGPGVVDPSGTGVWVQTRFRLDSYLGQRIRIRWIAET
                     WAFDETSSAYYPLGGIWAATMEDDGWWLDDIQVTGVLTRQLTPAIDTTPRTGACPGDP
                     CDAAAGDAGTSAVLEVADAAGAPIDGVTRIPTTGERIRLGAAASTFPGGCVDGHAEYR
                     FLRDGAVVQDWSANPFFTDSPERTTR"
BASE COUNT          963 a         2387 c         2212 g          913 t
ORIGIN      
        1 agaccgttcc cacgccgtcg ggcgggctcg cgatcgcggc cgcgcagttg cgcctcgcgc
       61 tcgcgtggat cgacctccct tcgccggaga acagcggtgg tcccctcatc aacgatctcg
      121 atctccggct ggagagcccc ggccccgatt cgtgccttgc ggcgggcgac acacggccgg
      181 atggcgccgt ctgccccgcc gggagcgcct ccgacaacgt ctattacgac ggcaacgact
      241 acgcctcggt gaacgacgac ccggcgctca gccagtggtc gcgcccgcgc gccgcctctc
      301 ccgaggtgca cgacacgcgc aacccggtgg aggcgatcca cctgaccgcc gatcccgaca
      361 acgatcgctc cttcgccgat tcggcgatct acgcgggacg ctggcgcgtc acggtgaagc
      421 gcgggctcgg cggggccacc ccggggtcga tcaccatcgt caccggcccc gacccggacg
      481 aggacgacaa caacaacggc cggctcgacg ccggcgagga tgagaacggc aacggcctgc
      541 tcgatctccc cgggcaggcg tacgcgctga tcgtgtcggg gccggtcttc gccgccgagc
      601 cgccgccggc cgcggggcct cagacgttcc cggcgagccg cctcagtctc gaccggaccg
      661 cctacgattg cggcgccggt gccgtcgcgt cgatcctgga caccacgccg gccgcgtcgc
      721 cggccctgtc ctcctcggcg acgaccttca cggtgctcgg ggcgaacggc tcggtgctcg
      781 ataccgagac cggcatcctg ttctccgccg cggggagtcc cggagcgacg acttcggcgg
      841 cggtgccggt gcggctggcc gggccggctc tcggcggcaa cgggatcctc gaagccgaca
      901 ccggccagag aatccgcgtc cgctacgctc cggcagggca gaccgcggtc gaggcgtcgg
      961 cggtggtccg ctgctccccc gacctgatcc gtgcctcctt ctcgacccag ggcgggcgct
     1021 cgatcggtga ccaggtgtcg atccaggggg ggtgcgacgg cgacgcgttc cccgacgccg
     1081 gggaggtggt gagctacggc gtcgccctgc agaaccgctc gcgcgaggcg cagttcgcgg
     1141 acgtcgtcgc gacgctgacg ccgtcggggc cgggggccgg ggcggtgcgg gtcctcgact
     1201 cgccgcgggc gctggggctt ctgccggacg gcgggtcgaa cgcggctttc ttccaggtct
     1261 acgtcgatcc ggcggtggtg caggcactcc cggtcgcgag ccgtgtcgtc accatgcgtc
     1321 tcgacctcga ctcgtcggag cgcggggtgc ggctgggaag gcagacctac accttcacgc
     1381 acgcgctcaa tgccgacagg gacgagcggc gctactccac cgacgccatc accggggcgc
     1441 gtgaggtgcg cgacctcaac cgcaacggcg tcatcgatcg ggcgggggag accgatccgg
     1501 ccctcggctt cgtgctgccg gcggaggacg cgaccttctc gtccctgttc agcggcagcg
     1561 gcgcgccggc cggccacttc accaacgagc tgggggagga tctcaatctc agcggcagcc
     1621 tggagttcgc cgagcgcgat ttcattccga acgcggccgt cgaccgcggc atcctcaact
     1681 cgaacaaccc cgcggatccg gcgcaccgcg ccccctggag cttcgacagc aacaacggcg
     1741 gctggctgtc gctcaggcac tccggcagca aggtcggtct cggcatcggc ccacttccga
     1801 tctgggagta caagacgaac ggccgatgcg gcttccagac ggccgggggg ctgaacaagt
     1861 tcggcgtctg gcacaccggc gacaccaacg ccgcgacccc cacggcgtcg tcgccatgcg
     1921 acaaccatcc ttcgccgtcg gacccctcca cgcccaacac gacggagtac ctgttcgaca
     1981 tcgccgtgtc tccgctcctc gccaaggtga accagtcggc cgacgcgcgc ggcttcccgt
     2041 ggaccgtcga gttccagcgc gtcggcctga acctcaacat ccagctgttc gacggttacg
     2101 ccggcggcgg ggtgaacgtg gacaacgacg tcgagtccga cggcgccaac agcctgctgg
     2161 cgcagagaag cgatccgtac tacgcgtcca acagcggcgg ctggccggtc gggctcttcc
     2221 ggttcgccta cgactatttc cccggggatg ggattgatcc ccagtcgctg cagccgcagc
     2281 gcacgttcgg ccccttcacg aaccctgacg gctccgcgac cctgaacggg gacgagaccg
     2341 gattcaccgg gtacaccacg aacacgaacc cctacagcac gagcccgatt ccggaagccc
     2401 cacccgactt cctgccgttc ccgcggcccg gccaggcgct ggcgggggtc tgcaccggag
     2461 gcggcgcggc cggaagcccc tgtgcgccgg gcgtgccggg agacccgtgc gtcgtgcagg
     2521 ggggcgcgtg cgcgccgcag accgacacgg tcgccggtcc ggtccgcaat ttcgacgcga
     2581 ccctgctcgg ctacgaagga ggcttcgcca gcctgaacaa cgggctggac gccgtggaga
     2641 acatcgtgac cgccctgccg gggcgggcgg ggaaccgctg gcagatcggc atcggcttct
     2701 gggcggtaga gagcgcctcg gcgttcaccg actatggcgt cgccttcgac gacgtcgtct
     2761 tcgaatggaa cgaatggcat cccgaggacg agagcgtctt gggcgtcccg cccgcctgct
     2821 cacgcttcgg cggcacgggg cagccggccg gcggcgcctg cgccaccgtc accgccgacc
     2881 ggacggcgct ttacgagtgc gacgagtcga tcgaagtgac ggtgcacgat gccaagtgcc
     2941 tcgcgatcgg atcgggcgcg acggcggcgc tcggtggtgc ctgcgtcgcc gacgccgact
     3001 gcggcgccgg agggatctgc accgccgccc gcccctcggt cgaagtggcg gtggtcaccg
     3061 acagcgacgc caacgccgtc gtggtggacg gccaggcggt cctgttcccg acctccaagc
     3121 gcttcgcgct gccggcggtc gccggcacgc ccggccttta caaggggcgg gtcgccgtca
     3181 cgacggtcga gaacgacgcc gcgcacctgt acgtggaccc cgcgaacgat cggcgcttcg
     3241 ccgtctacta cttcgacccg ctgtgcgacg gcgaccggga cggccaggcg ggagaggacg
     3301 gcttcgacaa cgccgacggc gacggcatcc cggacgcgtc cgacaagtgt ccgctcgtcc
     3361 acgacccggg ccaggcggac gccgacggcg acgggcgcgg cgacctgtgc gacaactgcc
     3421 cgaacgccgc caacgcgaac caggccgacg ccgacgccga cggcgcgggg gatgcctgcg
     3481 agttcgacga cctcgacggc gatctcgtcc cgaacctgaa cgacaactgc ccggacgtcc
     3541 gcaatctcaa ccagagcgac atcgacgcga acggtcgcgg cgacctgtgc gacacgctca
     3601 agacctcggg catcaccttc ggcggcagta ctgggatggc cgactgcgtc gccggaacct
     3661 gcgcgcgtcc gggtgacgcg gttggcgacg cctgcgtcac cgacgaggac tgcatccgcg
     3721 gctgccacaa caatctctgc acccagggca acagcggcac agcgggcggt ctgagctgcc
     3781 tcagcggacc gaacacgggc caggcctgca ccagcaacac gaactgctcc gtatgcgtcg
     3841 gcggcaccac tcccggggtg gcgtgcatca ccaacagttg cgtcagcgcc tgcgtcggtg
     3901 gcacgaatcc gggggcgggc tgcgccaacg acagggactg cgctggcggc ggtacgtgca
     3961 gcaacatcgg cacctgcgtc gtgggccttt gcttcatcaa cttcgttgcg ccgacaccga
     4021 cgatcgggca ggcctgtgtc acgcacgccg actgttacgc ggacctcgat cgggatgcgg
     4081 acggagtgat cgacgccctc gacaactgcg tcatcgccgc gaacgggccg ctcggaggac
     4141 cgagcaacca gatcgacagc gacctcgacg ggctcggcga cgcgtgcgat gccgactgcg
     4201 gcggcgcggc ggtggccttc cgctgccggg ccaatggcgc gccgtgcccc gctcccgaga
     4261 cgaaccagcc ggcggcctgc gcgaacccgt acggactcgg accggtctgc ggcttctacg
     4321 tcaccaacgc cggcggctgc tcgacgaggg atgacgacct cgatgccgac ggagtcggag
     4381 acgccttcga cgattgtccg gcgacggcca acgcggccgt gttcgccgga ggcccgcagc
     4441 gcgaccggga tcgggacggc ctgggggacg cctgcgatcc gcccgcggcg tacgacgaca
     4501 ccgccgacgg gctcccggac gatgtcgtga cgttccaggg agcgatcgcc tgtcacgccc
     4561 ggccgctcgc gagcctgacc ctcgtcggga tccccgccta cgtcgatttc gacggtgacc
     4621 acgaccagtt ccccgacacc ggggagcgcg gccgcgtctc catcaccgtc cgcaacgacg
     4681 gtccggccct cgacgacgcg gtcctgacgc tcgtctcgac cgaccccgac gtcgcctgca
     4741 tcaccgaggc acaggtcgtc gtgccgagcc tgcctgccgg cgtcaccatg acgatcggca
     4801 gcctcgaccc cggccagccc gggttcggct tcgtcgcgag caacgcgctg cagtcacagc
     4861 ccccgccggt gcctgtctcc cgcatcgatc tgcagatcca ggtcgcggcc ccggggattc
     4921 tcgggaacgc ggccccgatc gccgtctcgc tcttcgccga ctacaacatg ccgcccgggt
     4981 cgcagcagtt cgtcctcggt ccggatgggc tggcgggtac cgcggacgac ggcacggtga
     5041 tcgagagctt cgactccgac cgggacgagg acggcaacgt gaccgtgcgc gacacgttcc
     5101 tcgaggcggt cgcgccgggc gtgttccgcg gctcgtgcag caatgcgccg ctgaccgcct
     5161 gcgtcacggc ggccgactgt ccggcgagtc ccccgggggc ggtgtgccaa tcgggggcgt
     5221 acctgcgcgg catcgccggc ggctccggtc tcaaccgggt cgcggctgtg gtctgcggcg
     5281 gtttcgacgc ctattccttc ggtccgtgcg tcctggatcc ggatttcccg atggattggc
     5341 acctgcactg cgcgcccggc gccgccaact gtcccaacgt ggagtcgggg acctgcgtcg
     5401 gcggctgctc gttcaacacg ccgaccggcg gccagcgcgc gctgagcccc cccaactcgc
     5461 tgcacatggg ggcccacttc gttcccggtg acgcgctcgc gggggacacc actcatctcc
     5521 gcgcgctgca ggggttcatg accgccccgc tcaacctcgc gttcctgcct cggccgggcg
     5581 atctcgacct gtccttcttc cacatcgcgc gcctcatgga caacaacggc gtcgggccga
     5641 acaacgagca ccagtgcgtc gactgcgccg acgtgcagat ccaggtcgat cagaatcccg
     5701 atcccaacgt cgatgcgtgg ggcttctggg acaagatggc gccgtaccag aacgtctacg
     5761 accacaagcc ggtcgcctgg tccaccttcg gttcgtacta ctgcgtgttc accccggccg
     5821 acgccggcgc ggcgccgccg aacccgcgcg gcgtgcacga gacgatctgc tatccgcagg
     5881 gggcctggtc gcactgcgga tcgacgatcg gcaccactcc tgccgcgacc gtggactgcg
     5941 ccggcccggg ggtcgtcgat ccatccggca ccggagtctg ggtgcagacg cgattccgcc
     6001 tcgactccta tctgggacag cgcatccgca ttcgatggat cgccgagacg tgggccttcg
     6061 acgagacctc ttccgcgtac tacccgctcg gcggcatctg ggccgcgacc atggaggacg
     6121 acggctggtg gctcgacgac atccaggtga ccggcgtctt gacccggcag ctgaccccgg
     6181 cgatcgacac cactccgcgc accggcgcgt gccccggcga tccgtgcgat gccgccgcgg
     6241 gggacgcggg gacgagcgcg gtcctcgagg tggccgatgc cgccggggcg ccgatcgacg
     6301 gtgtcacgcg cataccgacc acgggggagc gcatccgcct gggtgccgcc gcctcgacct
     6361 tcccgggcgg ctgcgtcgac ggccacgcgg aataccgatt cctgcgggac ggcgcggtcg
     6421 tgcaggactg gagcgccaac ccgttcttca ccgacagccc ggagcgcacc acgcg
//
LOCUS       DASRIQ010000223         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3214072, whole genome shotgun sequence.
ACCESSION   DASRIQ010000223 DASRIQ010000000
VERSION     DASRIQ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3214072"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VFD06_05505"
     CDS             complement(<1..1000)
                     /locus_tag="VFD06_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZN03024.1"
                     /translation="MSLRLKLVLALAFAALLPMGVAAAVPWLQAERRAGEATARRLDL
                     IRRQAAILVERRKAETVSRMDQASSDLSTGRARIQDLLQGPVAAARSIVTDLKERYGL
                     DHLEARNERGAVLATTGPERGGALALEPEDLTDGAFGLRRLPAPSGGEEAPLLYVESR
                     SVRAGGEALTLVGGLIVGEAFVAGVSEILGEPARLVGGQAGTSDRDPGVEPAGSPSAP
                     GQNVVDVPLGGAGWMLRVSVQPEDARQVRRELRAAYSGLAPLVLGSALVVGLILAEGI
                     SRPIRGLVQRAERISAERSRGTVRAQGRDEVRRLTQSFDRMLQALSESERQRLAAER"
     gene            1135..2220
                     /locus_tag="VFD06_05510"
     CDS             1135..2220
                     /locus_tag="VFD06_05510"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HZN03025.1"
                     /translation="MAPPSRAAVLEFHQSLPGYAPTPLHSCHGLARALGVGGLYVKDE
                     AHRFGLKAFKGLGASWALQRVLKDGRAAGTSPPTAVATATDGNHGRAVAWAARRAGLA
                     ATIFIPAGSAPARVEAIRGEGARVILVEGTYDDAVRQCAEESGRNGWQVIADTGYEGY
                     LTIPRLVAEGYSTMFTEADSQIEMASLARPGLLIVQGGVGALACAAVEHYRGSSDRPA
                     IAVVEPVDADCLLESIASPDGRPRPARGRQASIMAGLNCGEVSLAAWPTLRGGVDLFF
                     SIEDRHAEEAMRRLHHAVTGDPPIEAGESGAAGLAGLVALQTEEALRGARERLSLSDR
                     TVVLVLNTEGATDPEAFRRIAGLGPLP"
     gene            complement(2186..>2601)
                     /locus_tag="VFD06_05515"
     CDS             complement(2186..>2601)
                     /locus_tag="VFD06_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HZN03026.1"
                     /translation="IVRKLAEMGGDMAVHDPYVLRWWEFEEQKTYPRPGYSWSRFFRN
                     QEGLKALRVQQDLGAALKGADAAILAVRHKAYLDLSPDEVVRMAGRTLCVIDCFGILD
                     DAKIRRYFELGCEVKGLGRGHIQRIKEEVRGRRSS"
BASE COUNT          361 a          966 c          913 g          361 t
ORIGIN      
        1 cccgctccgc cgccaggcgc tgccgctcgc tctcggagag cgcctggagc atccggtcga
       61 aggactgcgt cagccgccgg acctcgtccc tgccctgcgc ccgtacggtc cctcgcgatc
      121 gctcggccga gatccgctcc gcccgctgca ccaggccgcg gatgggccgg gagatcccct
      181 ccgccaggat gaggccgacg acgagggcgc tccccaggac gagcggggcc aagccggagt
      241 acgcggcacg cagctcgcgc cgcacttggc gcgcatcctc cggctgcacc gagacgcgca
      301 gcatccagcc ggcaccgccg aggggcacgt ccacgacgtt ctgaccgggg gccgaaggcg
      361 agcctgccgg ttcgacgccc ggatcgcggt ctgaggtgcc tgcctgtccg ccgacgaggc
      421 gcgccggctc gccgaggatc tccgacacgc cggccacgaa cgcctcgccg acgatcagtc
      481 ccccgacgag ggtcagcgcc tcgccccccg cgcgcaccga gcggctctcc acgtacagaa
      541 gaggcgcctc ttctccgccg gagggcgcgg gcagacgccg cagcccgaaa gccccgtccg
      601 tcaggtcctc gggctcgaga gccagggcgc cgccgcgctc cggccccgtc gtcgccagga
      661 cagcgcccct ttcgttcctc gcttcgaggt ggtccaggcc gtagcgctcc ttgaggtcgg
      721 tcacgatgct gcgcgcggcc gcgacgggcc cctggagcag gtcctggatg cgggcgcgtc
      781 ctgtcgagag atccgacgat gcctggtcca tgcggctgac cgtctccgcc ttccgcctct
      841 cgaccaggat cgccgcctgc cgtcggatga ggtcgagccg ccgcgcggtc gcttcgccgg
      901 cccgccgttc ggcctggagc cagggcaccg ccgcggccac ccccatcggc agaagcgctg
      961 cgaaggcgag ggccaggacg agcttcagcc gcaggctcat cttccggaga ctctacgtca
     1021 gggccggtcg ttccgtccac atccggacac cacacgatcc gcatcaccat gtaatctctt
     1081 gtcgtgagcc cgatgcggtt cgtcttcaat ccctttctgc gccaaggcga ggccatggcg
     1141 ccaccgtcgc gagcggcggt gctggagttc caccaatcac tccccggcta cgccccgacg
     1201 ccgctgcact cctgccacgg cctggcgcgg gcgctcggcg tcggcggact ctacgtcaag
     1261 gacgaggcgc accgcttcgg actgaaggcg ttcaaggggc tgggggcatc ctgggcgctg
     1321 cagcgtgtcc tgaaagatgg ccgcgcggcg ggaacgtcac ccccgaccgc cgtcgctacg
     1381 gcaaccgacg gcaaccacgg gcgcgccgtg gcctgggcgg cccggcgcgc cgggctcgcg
     1441 gcgacgatct tcattcctgc cgggtcggcc ccggcgcgcg tcgaggcgat ccgcggcgag
     1501 ggggcgcgcg tcattctggt cgaggggacc tacgacgacg ccgtgcgcca gtgcgccgaa
     1561 gagagcggca ggaacggctg gcaggtcatc gccgacacgg gctacgaggg gtatctcacc
     1621 atcccgcgac tcgtggccga gggctacagc accatgttca cggaggccga cagccagatc
     1681 gagatggcgt ccctggcgcg ccccgggctc ctgatcgtcc agggaggcgt gggcgctctg
     1741 gcgtgcgccg ccgtcgagca ctaccgcgga agcagcgacc ggccggcgat cgccgtcgtc
     1801 gagccggtgg acgccgactg cctcctcgag tcgatcgcct cccccgacgg ccgcccgcgg
     1861 ccggcgcgcg ggcgccaggc ctccatcatg gcgggcctca attgcgggga ggtgtcgctg
     1921 gcggcgtggc ccacgctgcg gggcggggtg gaccttttct tcagcatcga ggaccgccat
     1981 gccgaggagg cgatgcgccg gctgcaccac gcggtcacgg gcgatccgcc gatcgaagcg
     2041 ggcgagtcgg gtgcggccgg gctcgcgggg ctcgtcgccc ttcagaccga ggaggccctg
     2101 cgcggcgccc gggagcggct ctccctatcg gatcggacgg tggtgctggt tctgaacacc
     2161 gagggggcca ccgaccccga ggcgttcagg aggatcgccg gcctcggacc tcttccttga
     2221 tgcgctggat gtggccgcgc ccgaggccct tgacctcgca gccgagctcg aaataacgcc
     2281 ggatcttggc atcgtcgagg atgccgaagc agtcgatgac gcacagggtt cggcccgcca
     2341 tccggaccac ctcgtcgggg gagaggtcga ggtaggcctt gtggcgcacc gcaaggatcg
     2401 ccgcgtcggc ccccttgagg gccgccccga ggtcctgctg gacgcgcagc gccttgagcc
     2461 cctcctggtt gcggaagaag cgcgaccagg aatagccggg ccgcgggtag gtcttctgct
     2521 cctcgaactc ccaccagcgc aggacgtacg gatcgtgcac cgccatgtcg ccgcccatct
     2581 cggccagctt gcgcacgatc a
//
LOCUS       DASRIQ010000224         8680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1148806, whole genome shotgun sequence.
ACCESSION   DASRIQ010000224 DASRIQ010000000
VERSION     DASRIQ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148806"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..779
                     /locus_tag="VFD06_05520"
     CDS             195..779
                     /locus_tag="VFD06_05520"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN03027.1"
                     /translation="MAAEPTDLELVQEVLAGRIEQFEILVRRYQRLVATAALRMGIPR
                     QEIEDVANEVFYKVFRSLHRYEPSHALSTWLYRITVNAALDHKRSRRHDAKSDELSPA
                     LADDRPGQEETTGDRERAALLREALQRLPDHYRTPLVLTHVEGLPIEEVAKALNLPEG
                     TVKSRLFRARAQLKEILRRHYPGLVPPGLVEASS"
     gene            776..1330
                     /locus_tag="VFD06_05525"
     CDS             776..1330
                     /locus_tag="VFD06_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03028.1"
                     /translation="MKHEMQDDRELALWTEATLAPLRRQMAEVDVTGSVMQRIAASRP
                     RPVASLLPVRWQRPAWAATLVAGFAAFVLLSTTAVLMLANGDPGARDAMTLAATGGSL
                     LLHGLNAAWGVVKALAETAFALGRGGFTLLDAASPLVRAAGVLAAFCGAASIAFSALI
                     VSRAQRHAPLVSRASWNILNGGFS"
     gene            1327..2337
                     /locus_tag="VFD06_05530"
     CDS             1327..2337
                     /locus_tag="VFD06_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03029.1"
                     /translation="MSRPGSLTTVRALALGLLVALSALLPVSPLFASGGQAVSVSDDS
                     YVPAGKVHQGDLVSVMGDVKVEGEVTGTVVVVLGSVEIQGKVDGDLVSIMSRAELGDA
                     ASVDGEFVNVGWTPIRSSGSSIGGEIVNVNFMNLIPFAGHGGGISGLLRFLFILSLIK
                     LALLFMAMVIITSLIPRRLAVMAAAFPTKWGWSFVVGLLTYVGIWIGVLFLAVTLIGI
                     PLALFLWFAGKAIKWMGLAAILYLMGQTMGRNLWGRQLPHLASVLAGFVVFAVASMLP
                     GLGWIFSGVLSVLALGLAVTTRFGSEPAVAGAAAQGPTPPPVWEAAPANGAPTPGPGT
                     TA"
     gene            2433..3008
                     /locus_tag="VFD06_05535"
     CDS             2433..3008
                     /locus_tag="VFD06_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZN03030.1"
                     /translation="MFHRDARLGIPSAFVAACGTAPPSGYASNFTDCDDSTAQARPGG
                     TEYAGDEIDGNCDGTEVCFLDDDNDGYLTGQIVLSSDTDCADSGEGRFTEPAGDCCDQ
                     NADARPGQTLFFSVSNGCGGYDYNCDGIHTQQFTQVSTTTLACGGSPPFCVDLGTGWV
                     TSVAFCGVTANYRDCSSTCGTTIFPLTQPCR"
     gene            complement(3024..4385)
                     /locus_tag="VFD06_05540"
     CDS             complement(3024..4385)
                     /locus_tag="VFD06_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN03031.1"
                     /translation="MIARLLRRAADVKDGEVGPVLWACLYCFFVLASFYVLRPLREEM
                     GIAGGIKNLPRMYLGTLAATIVMVPLFSWLVSRFSRRRFIPIAYRFFALNLVGFYLLF
                     RWLPEDSHVIAARVFYIWLSVFNLFVVTVFWGYMADIFTSGQGKRLFGFIAAGGTLGA
                     VAGAGFTNQIVERVGTHAMFFIAAFVVEAAVQAFRRLDRLPVFLGPEGNLSLDAVAVK
                     VAAAAVKKEERLDRTGFLGGFTLVVRSPYLLAYCLFMLLFTNSSTFAYFEQARFVKAA
                     SASPEARTAIFAQMDLLVNLLALFVQMFLTGRVVRWLGLGGALCILPLLSLSGFGALL
                     VWPTLVMLMTFQVLRRGTDYAVSKPTREVLFTVLPREEKYKAKSFIDTFVYRAGDAIN
                     AAVYGQLTESGDPARRVALVSVPVTAAWIAVSLMLSRRQQRLAATVAPVPVAPVTRPA
                     PAV"
     gene            complement(4382..5575)
                     /locus_tag="VFD06_05545"
     CDS             complement(4382..5575)
                     /locus_tag="VFD06_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN03032.1"
                     /translation="MSGIDDRDEQDRFRGGSRRSFLKNAAAAAALAGLGAQGLAFGKD
                     KSKKGEKAKKGKGMSILILGGTRFLGPATVEAALKRGHTVTLFNRGKSDPGRFPNLET
                     LLGDRDGNLKALEGRKWDAVVDTSGYVPRVVRDSVNLLKDNVGHYVFISTLSVYSDNS
                     KPWMDESGPLMTVEDPNNESVKENYGALKALCEQAAEKIMPGRVANIRPGLIVGIDDP
                     SDRFTYWPVRVAKGGDVLAPGTPADPVQFIDVRDLGEWTVKVIEDKTVGVYNATGPNT
                     GPKDEVGMGMLLDACNKNGGGKSNFVWCSADFLAEQKVEAWSDMPVWVPPSGDSAGFA
                     RTSCAKAIAKGLTFRSTPDTVKSTLDWFRTEPAEHQAKLKTGIAPERGAEVLAAWRAK
                     QQPQG"
     gene            complement(5667..7085)
                     /gene="boxB"
                     /locus_tag="VFD06_05550"
     CDS             complement(5667..7085)
                     /gene="boxB"
                     /locus_tag="VFD06_05550"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HZN03033.1"
                     /translation="MTSIDLDATIPNNVDLSTDKRLQRALEQWQPSFREWWKGMGPDR
                     FQDRDVYLRTAIAVDSAGWAHFDYVRMPEYRWGIFLAAPEADRKIGFGDNMGQPVWQE
                     VPGEHRNALRRLIVTQGDTEPASVEQQRQLGATAPSLYDLRNLFQVNVEEGRHLWAMV
                     YLLHAYFGRDGREEAEALLARRSGDPDKPRILGAFNAPIEDWLSFFMFTMFTDRDGKF
                     QLLALAESGFDPLSRTTRFMLTEEAHHMFVGETGVRRIVQRAAELTKEAKGGDVRALG
                     GIDLATLQKYVNDWYALSLDLFGGEISSNAADYFANGLKGRANEKKHPDHLALEGVYS
                     MQVPEGSRLVTQEVPMRNALNEVIRDAYIDDNQRAVDQWNKTLADNGVDFRIRLPFRG
                     FNRRIGLYANLNATPDGTLVSREEWERRKDEWLPSESDRAYVASLMQPVYEPGKIANW
                     IAPPTKGINNQPLEFEYVRLKG"
     gene            complement(7088..>8680)
                     /gene="boxC"
                     /locus_tag="VFD06_05555"
     CDS             complement(7088..>8680)
                     /gene="boxC"
                     /locus_tag="VFD06_05555"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HZN03034.1"
                     /translation="TLGMDVQEDAGLVSDYRLKLNSYDLGVDIELADAVERLRFEHPE
                     VRVVVVTSGKDRVFSAGANIHMLASSSHAFKVNFCKFTNETRLAIEDASAHSGIRFLA
                     ACNGIAAGGGYELALACDEILLVDDGNSAVSLPEVPLLGVLPGTGGLTRVVDKRRVRR
                     DHADIFCTLAEGVKGQRAVDWRLVDATAPRSRFDAAVKERAAALAAADRGRVAPPRRG
                     PGVALPPLEPDIAKDLWAYSTLGIAFDRKARTATVTLKGPDGPPPEDVEAAHKLGANL
                     WMLRLSRELSDALCRLRFNEPEVGLLVIKTLGSVDAVLQHDARMAALAQTDWYAREVV
                     LHFARTLRRLDVTARSLFALVEPGSCFAGSLLETALAADRTYALDDPKRKVEMALSPL
                     NFGALPMGHDLTRLEVRLLSRPADHAALRGRIQAGETPKYDAPGAVAAGLVTVAADDI
                     DYEDEVRVAIEERASLSPDALTGMEASLRFPGAETPETKIFGRLSAWQNWIFQRPNAV
                     GEHGALKCYGRPERPAFDWTRT"
BASE COUNT         1305 a         3024 c         2974 g         1377 t
ORIGIN      
        1 cggggtgcgg gactcgggga tcgggcggga gggcccccgc tattccatgc acgaaatgtc
       61 ggaaccgaaa ctggtcctgt tcaacctgaa ccactgacgc gggcgggcgg gattttccgg
      121 cctgatccgc atatatcgca ggaaccggcc gcccgggccg ccggtactat ggggacggac
      181 cgaacagacc ctcgatggcc gccgagccga ccgacctcga gctggtccag gaggtcctgg
      241 cgggaaggat cgagcagttc gagatcctcg tccgccggta ccagcgcctg gtggccaccg
      301 ccgctttgcg catggggatc ccccgtcagg agatcgagga cgtggccaac gaagtctttt
      361 acaaggtttt ccggagcctg caccgctacg agccgtcgca cgccctctcc acctggctct
      421 accggatcac cgtcaacgcc gctctcgacc acaagcgcag ccgccgccac gacgccaagt
      481 cggacgagct ctcccccgcc ctggccgacg accgcccggg ccaggaggag acgaccggtg
      541 accgggagcg ggcggccctg ctccgggagg ccctgcagcg cctcccggac cactatcgga
      601 cgcccctcgt gctgacgcac gtcgaggggc tgccgatcga ggaggtggcc aaagcgctca
      661 acctgccgga agggacggtc aagtcgcgtc ttttccgggc gcgggcgcag ctcaaggaga
      721 tcctccgtcg ccactatccg ggcctggtgc cgcccggcct ggtcgaggcg tcgtcatgaa
      781 gcacgaaatg caggacgacc gggaactagc cctctggact gaggcgaccc tggcgccgct
      841 gcggcggcag atggccgagg tggacgtgac cgggtcggtg atgcagcgca tcgccgcctc
      901 ccggccgcgg ccggtggcct cgctcctccc ggtccgctgg cagcgtccgg cctgggcggc
      961 gacgctggtc gcgggattcg ccgccttcgt gctgctttcg acgaccgccg tcctgatgct
     1021 ggcgaacggc gacccggggg cgcgcgacgc catgaccctg gcggcgaccg gcgggagcct
     1081 gctgctccac ggtctcaatg ccgcatgggg agtggtcaag gcgctcgcgg agaccgcctt
     1141 cgcgttgggc cgcggcggct tcaccctcct cgatgccgcc tccccgctcg tgcgggcggc
     1201 cggagtgctg gcggccttct gcggcgccgc ctcgatcgcc ttcagcgccc tcatcgtctc
     1261 ccgggcgcag cggcacgcgc cgctcgtctc ccgcgcttcc tggaacatct tgaatggagg
     1321 attttcatga gccgtcccgg ttccctgacg accgtccgcg ccctcgcgct cggattgttg
     1381 gtcgcccttt ctgccctgct gccggtctcc ccgctcttcg cctccggcgg ccaggcggta
     1441 tcggtgtcgg acgactcgta cgtccctgcc ggcaaggtcc accaggggga cctcgtctcc
     1501 gtcatggggg acgtcaaggt ggaaggcgag gtgacgggaa cggtggtggt cgtcctcggc
     1561 tccgtggaga tccaggggaa ggtggacggc gacctcgtct cgatcatgtc gcgcgccgag
     1621 ctgggagacg cggcctcggt ggacggcgag ttcgtcaacg tcggctggac ccccatccgc
     1681 agctccggga gcagcatcgg cggcgagatc gtcaatgtga acttcatgaa cctgatcccg
     1741 ttcgccgggc acggcggggg gatcagcggg ctcctgcgct tcctgttcat cctgagcctc
     1801 atcaagctgg cgctgttgtt catggcgatg gtgatcatca cctcgctcat cccgcggcgc
     1861 ctggcggtga tggcggccgc cttcccgacg aagtggggct ggtcgttcgt cgtggggctc
     1921 ctgacctacg tcgggatctg gatcggagtg ctcttcctcg cggtgaccct catcggcatc
     1981 ccgctggcgc tgttcctctg gttcgccggc aaggcgatca agtggatggg gctcgccgcc
     2041 atcctgtacc tcatgggcca gacgatggga cggaacctct ggggccggca gctgccacac
     2101 ctcgcgagcg tgctcgccgg gttcgtcgtg ttcgccgtcg cttcgatgct cccgggcctc
     2161 ggctggatct tcagcggtgt cttgagcgtg ctcgccctcg ggctcgccgt caccacccgc
     2221 ttcggatcgg agccggcggt cgccggcgcg gcggcgcagg ggccgactcc gccgccagtc
     2281 tgggaagcgg cccccgccaa cggcgccccg acgcccggcc ccggcaccac cgcctagacc
     2341 cggcgacacg tttcggtccg ctccgtcacc cgcatcaggc agacctgata cgggaggatg
     2401 gagatgggct cgaaccttcg cttcgccctg acgtgttcca ccgcgatgct cgcctcggca
     2461 tcccctccgc cttcgtcgcg gcgtgcggca cggccccgcc gagcggctat gcctccaact
     2521 tcaccgactg cgacgactcg actgcccagg cccgccccgg cggaaccgag tacgcgggcg
     2581 acgagatcga cgggaactgc gacgggaccg aggtctgctt tctggatgac gacaacgacg
     2641 ggtacctgac cggtcagatc gtcctgtcgt ccgacaccga ctgcgccgac tcgggggagg
     2701 gtcgcttcac ggagccggcc ggcgactgct gcgaccagaa cgccgacgcc cgtcccggcc
     2761 agacgctctt cttcagcgtg tccaatgggt gcggcggcta cgactacaac tgcgacggca
     2821 tccacacgca gcagttcacg caggtctcga cgacgaccct ggcatgtggc ggctcgccgc
     2881 ctttctgcgt cgatctcggg accggctggg tgacgagtgt cgcattctgc ggcgtgaccg
     2941 cgaattatcg cgactgcagc tcgacatgtg gcacgacgat tttcccgctc acgcagccct
     3001 gccgctgaga accggaagcc ggatcagacc gcgggcgcgg gccgggtgac cggggcgacg
     3061 ggcaccggcg cgaccgtcgc cgccaaccgc tgctggcgcc gcgacagcat cagggagacg
     3121 gcgatccagg cggcggtgac cgggacgctg acgagggcca cgcgtcgcgc cggatccccg
     3181 gactccgtca gctgcccgta aacggcagcg ttgatcgcat cgccggcgcg gtacacgaag
     3241 gtgtcgatga aggacttcgc cttgtacttc tcctcgcgtg gcagcaccgt gaagagcacc
     3301 tcgcgcgtcg gcttgctcac cgcgtaatcg gtcccgcggc gcagcacctg gaaggtcatc
     3361 agcatcacga gagtcggcca cacgagaagc gctccgaacc cgctcaggct caggagcggc
     3421 aggatgcaca acgccccgcc gagcccgagc cagcgcacca cccgtccggt gaggaacatc
     3481 tggacgaaaa gcgccaggag gttcacgagg agatccatct gcgcgaagat ggcggtgcgc
     3541 gcttcggggc tcgcggaggc ggctttcacg aaccgggcct gctcgaagta ggcgaaggtc
     3601 gatgagttcg tgaacagcag catgaagagg cagtaggcaa gaagataagg cgagcggacg
     3661 accagggtga accctccgag gaacccggtc cggtcgaggc gctcctcctt cttcaccgcg
     3721 gccgcagcga ctttcacggc gactgcgtcg agcgacaggt tcccctcggg ccccaggaag
     3781 acagggaggc gatcgagccg ccggaaggcc tgcaccgccg cctcgaccac gaacgcggcg
     3841 atgaagaaca tcgcgtgcgt gccgacgcgc tccacgatct ggttggtgaa tcccgccccc
     3901 gcgacggctc cgagcgttcc tccggccgca atgaacccga acagccgctt cccctggccg
     3961 ctcgtgaaga tgtcggccat gtacccccag aagaccgtca ccacgaagag gttgaagacg
     4021 ctcagccaga tgtagaagac gcgcgccgcg atgacatgcg agtcctcggg aagccaccgg
     4081 aacaggaggt agaagcccac gaggttcagc gcgaagaagc ggtaggcgat cgggatgaag
     4141 cgccggcggc tgaagcggct gacgagccag gagaagagcg gcaccatgac gatggtcgcc
     4201 gcgagcgtgc cgaggtacat cctcggcagg ttcttgatgc cgccggcgat ccccatctcc
     4261 tcgcgcagcg gccgcagcac gtagaacgac gccaggacga aaaagcagta gaggcaggcc
     4321 cagagcacgg ggccaacctc gccgtccttg acgtcggcgg cgcgccgcag gaggcgggcg
     4381 atcacccttg cggctgctgc ttggcccgcc aggcggcgag gacctccgcc ccacgctccg
     4441 gggcgatccc ggtcttgagc ttcgcctggt gctcggccgg ctcggtgcgg aaccagtcga
     4501 gggtggactt caccgtgtcg ggcgtcgagc ggaaggtgag ccccttggcg atcgccttcg
     4561 cgcaggaggt gcgggcgaag ccggcgctgt cgccgctcgg cggcacccag accggcatgt
     4621 cggaccaggc ctcgaccttc tgctccgcca ggaagtcggc gctgcaccag acgaagttcg
     4681 acttgccgcc gccgttcttg ttgcaggcgt cgaggagcat ccccatgccg acctcgtcct
     4741 tggggccggt gttcgggccg gtggcgttgt agacgccgac cgtcttgtcc tcgatcacct
     4801 tgacggtcca ttcgcccagg tcgcgcacgt cgatgaactg caccggatcg gccggcgtgc
     4861 cgggggcgag gacgtccccc cccttcgcca cccgcaccgg ccagtaggtg aaacggtcgc
     4921 tcgggtcgtc gatgccgacg atgagcccgg ggcggatgtt ggccacgcgc cccggcatga
     4981 tcttctcggc cgcctgttcg cacagcgcct tcagggctcc gtagttctcc ttgacgctct
     5041 cgttgttcgg gtcctcgacg gtcatcagcg gcccgctctc gtccatccag ggcttgctgt
     5101 tgtcggagta gaccgagagg gtcgagatga agacgtagtg gccgacgttg tccttgagga
     5161 ggttgaccga gtcccgcacc acgcgcggca cgtagccgga ggtgtccacg accgcgtccc
     5221 acttgcgccc ctcgagcgcc ttgaggttgc cgtcccggtc gccgaggagg gtctcgaggt
     5281 tcgggaatcg ccccgggtcc gacttgccgc ggttgaagag ggtcacggtg tggccgcgct
     5341 tcagcgccgc ctccaccgtc gccggcccga ggaagcgggt gccaccgagg atgaggatcg
     5401 acatcccctt ccccttcttc gccttctccc ccttcttcga cttgtcctta ccgaaggcca
     5461 gcccctgggc tcccaggccc gcgagggcgg ccgcggccgc cgcattcttc agaaagctcc
     5521 ggcgcgaccc gccacggaac cggtcctgtt cgtcgcgatc gtcgatcccg ctcatctgat
     5581 ccgcctcctc ccgatgggcc ccgggcgaac gaccacctct ccccgccggg gacggccatt
     5641 ctaacgaagg ccatcccccg gaggtttcag cccttcaggc ggacgtattc gaattcgagc
     5701 ggctggttgt tgatcccctt ggtcggcggg gcaatccagt tcgcgatctt gcccggctcg
     5761 tagaccggct gcatcaggga ggcgacgtac gcacggtccg actccgacgg cagccactcg
     5821 tccttgcggc gctcccactc ctcgcgggac acgagtgtgc cgtcgggggt ggcattcagg
     5881 ttggcgtaga ggccgatgcg gcggttaaag ccgcggaacg gcaggcggat gcggaagtcg
     5941 acgccgttgt cggcgagggt cttgttccac tgatcgaccg cccgctggtt gtcatcgatg
     6001 taggcgtcgc ggatcacctc gttgagggcg ttgcgcatcg gcacctcctg ggtgacgagg
     6061 cggctgccct ccggcacctg catcgagtaa accccctcga gcgccaggtg gtccggatgc
     6121 ttcttctcgt tcgcccgtcc cttgaggccg ttggcgaagt agtcggcggc gttcgaggag
     6181 atctccccgc cgaacagatc gagcgacagc gcgtaccagt cgttcacgta tttctgcagc
     6241 gtcgcgaggt cgatgccgcc gagcgcccgg acgtcccccc ccttcgcctc cttcgtcagc
     6301 tcggcggcgc gctgcacgat gcggcgcacg ccggtctcgc cgacgaacat gtggtgcgcc
     6361 tcctcggtca gcatgaagcg cgtggtgcgc gacagcggat cgaaaccgct ctcggcgagc
     6421 gccaggagct ggaacttgcc gtcccggtcg gtgaacatgg tgaacatgaa gaaggagagc
     6481 cagtcctcga tcggcgcgtt gaaggcgccg aggatgcgcg gcttgtccgg atcgccggag
     6541 cggcgcgcca ggagcgcctc cgcctcctcg cggccgtccc gtccgaaata ggcgtgcagc
     6601 aggtacacca tcgcccagag gtgccgtccc tcctcgacgt tcacctggaa gagattgcgc
     6661 aggtcgtaga ggctcggcgc ggtggcgccg agctgccgct gctgctcgac cgaggccggc
     6721 tcggtgtccc cctgcgtgac gatgaggcgc cgcagggcgt tgcggtgctc ccccgggacc
     6781 tcctgccaca ccggctgccc catgttgtcg ccgaagccga tcttccggtc ggcttccggg
     6841 gcggcgagga agatgcccca gcggtactcc ggcatccgga cgtagtcgaa gtgggcccag
     6901 ccggccgaat cgacggcgat ggcggtgcgc agatagacgt cccggtcctg gaagcggtcc
     6961 ggccccatcc ccttccacca ctcgcggaag gagggctgcc actgctcgag cgcccgctgc
     7021 aggcgcttgt cggtcgagag atcgacgttg ttcgggatgg tggcgtcgag atcgatgctg
     7081 gtcatgtcta ggtcctcgtc cagtcgaagg ccgggcgctc cggccggccg tagcacttga
     7141 gcgccccgtg ctcgccgacc gcattggggc gctggaagat ccagttctgc caggcggaga
     7201 gacggccgaa gatcttggtc tccggcgtct cggcgccggg gaagcggagc gacgcctcca
     7261 tgccggtgag cgcgtcgggc gacagcgacg cccgctcctc gatcgccacg cggacctcgt
     7321 cttcgtagtc gatgtcgtcc gccgcgaccg tgacgagccc ggcggcgacc gcccccggcg
     7381 cgtcgtactt cggggtctcc cccgcctgga tcctgccgcg cagcgccgcg tggtcggccg
     7441 gccgcgacag cagccgcacc tcgagccgcg tcaggtcgtg tcccatcggc agggcgccga
     7501 agttcagggg cgacagcgcc atctccacct tgcgcttcgg atcgtcgagc gcgtaggtgc
     7561 gatcggccgc cagcgccgtc tcgaggagag agccggcgaa gcatgagccg ggctcgacga
     7621 gggcgaagag tgaccgggcg gtgacgtcga ggcggcgcag cgtgcgggcg aaatgcagga
     7681 ccacctcgcg cgcgtaccag tcggtctggg cgagcgccgc catcctcgcg tcgtgctgca
     7741 ggaccgcgtc gacgctcccg agcgtcttga tcaccaggag ccccacctcc ggctcgttga
     7801 aacgcagccg gcacagggca tccgacagct cgcgcgacag tcgcagcatc cagagattcg
     7861 cgccgagctt gtgggcagct tcgacgtcct ccggaggagg accgtccggc cccttgaggg
     7921 tgacggtcgc ggtgcgcgcc ttgcgatcga aggcgatgcc gagggtggaa taggcccaca
     7981 ggtccttcgc gatgtccggc tcgaggggcg gcagcgccac ccccggcccg cgccgcggcg
     8041 gcgccacgcg gccgcggtcg gcggcggcga gagcggcggc ccgctccttc acggcggcgt
     8101 cgaagcggct cctcggcgcg gtcgcgtcca ccagcctcca gtccaccgcc cgctgcccct
     8161 tcaccccctc ggcgagggtg cagaagatgt cggcgtggtc ccgccgcacg cgccgcttgt
     8221 ccaccacgcg cgtcaggcca ccggtccccg gcagcacgcc gagcagcggc acctccggca
     8281 ggctgaccgc cgagttgccg tcgtccacga ggaggatctc gtcgcaggcg agcgccagct
     8341 cgtagccgcc gccggcagcg atgccattgc acgccgcgag gaagcggatg ccggagtgcg
     8401 ccgacgcgtc ttcgatcgcg aggcgggtct cgttggtgaa cttgcagaag ttgaccttga
     8461 aggcgtgcga cgaggaggcg agcatgtgga tgttcgcccc ggcggagaag acccggtcct
     8521 tgccggaggt gacgacgacc acccgcacct ccgggtgctc gaagcgcaac cgctcgacgg
     8581 cgtcggccag ctcgatgtcc acgccgaggt cgtacgaatt gagcttgagg cggtagtcgg
     8641 agacgagccc cgcatcttcc tgcacatcca tcccgagcgt
//
LOCUS       DASRIQ010000225         5931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2719752, whole genome shotgun sequence.
ACCESSION   DASRIQ010000225 DASRIQ010000000
VERSION     DASRIQ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719752"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VFD06_05560"
     CDS             <1..1263
                     /locus_tag="VFD06_05560"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZN03035.1"
                     /translation="SRNPVFILDEVDKLGMDFRGDPASALLEVLDPEQNRNFQDHYLD
                     VPFDLSAVLFITTANILDTIPGPLRDRMEVLELPGYTEEDKLAIARLFLIPRQTTENG
                     ITVDDISLSDETLRAVISGYTREAGLRNLERSIGTLCRKVARGRAEGETGLVVITPDD
                     LFSYLGPERFTSELAERIAQPGVAVGLAWTPFGGEILFVEATRMPGSKGLIITGQLGD
                     VMRESAMAALSFVRSRAKSLGIDPDFFSQYDLHVHVPAGAIPKDGPSAGVTMATALAS
                     LLTGRPVRGETAMTGEITLRGKVLPVGGIKEKVLAAKRAGIRTILVPADNRKDLSDVP
                     KEALDALTFHFVSTVDEVLANALQPTPAEEPATPKTVDEETTPADDAAGSSPEVGDKG
                     RAVAAGKTSRPLPSGKRRVPNRGPDVTP"
     gene            complement(1307..4012)
                     /locus_tag="VFD06_05565"
     CDS             complement(1307..4012)
                     /locus_tag="VFD06_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN03036.1"
                     /translation="MSSSAAAPTPLTPADRVERLRRFGGRRAYLWRDGVRWRERSYAE
                     LHAGVLAVAAELRAAGLKSKEPVLVQGPDEPDWVEGLLGIFLAGGVAVPLEAATADPF
                     REKIASTVGARLLLAPASIPAPAGIRRIEFGAWRRAHPDGSAGGARSSAAAAAALTDT
                     AEIVFTSGTTGEPKGVVLTHANLAADFAPLEEIFRHYERIIVPLGEFRVLSTLPLSHM
                     FGQAMNVFLSLYMGLTVVLVAPRPRDVLDAATRVGAWGLFSVPRLLELLAAEIRREAG
                     HADAQARLDARLAQHAGKLFVLQRLLFRQVRRSLGWRFTVVVSGGAALADPVREFYER
                     IGVLVVQGYGLTETAPIVSISNPFQRGGRGDVGRALKGQEVRLGPDGEIQVRGANVTS
                     GYFGQKEGGEWLGTGDIGEIDAQGRIAIKGRIKDVIVTPEGENVYATDVEAAFFGLPG
                     LRDAAAFGLPHATGEQVHAALLLAPGADAAALVTEANRRLLPKQRVRDWTVWTEGDFP
                     RTSTGKVKRVALRASVMAGQKETPGGERRASAAGGVRPIIARIARVPAERLEDGTRLV
                     EDLGLASLDLVEVAAALEEDFGLTIAEDGMATATVGELEARARTALSGRREFGAAAPA
                     PAVRPTTADAGPSSAPDSEPAPAAVAPSNRDGGTGGAASWSARVIRGALRMPRWARSW
                     PMHVNRRLVEEALQRPIVHVWGRPDIRGRERLEAASPPYLFVANHHSYFDTALLRSTL
                     PRPMRGRIAPAMTTRYHRVWFGETAGTRGQYLLEGLQAGLTAFFFNAFPLPETAGFRR
                     SVVYAGELMDAGFSILIFPEGRHVPEGTMERFRGGIGVFARELRAPVVPVHVSGTHHV
                     LPDERYWPAFHPTRIVYGAPMTFAADADPTQVTQRLEAAVRELSG"
     gene            4127..4675
                     /locus_tag="VFD06_05570"
     CDS             4127..4675
                     /locus_tag="VFD06_05570"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HZN03037.1"
                     /translation="MPHHFSLDDFVAEMAHLSCENLTIGILHQLAGDLDVRDDLIREH
                     IHFTNDAYTRNLVCRTPRFDMLVLCWKPGHVTTIHDHAGSLNVTRVLQGRLTSRLFVP
                     GGRPTPDRCLVRLASEDQLGPGDFSLVDRAEIHQLANTSDADLVTLHVYAKPLRDIVV
                     YDPATGDMKKVALRYSLEDDFQ"
     gene            complement(4678..>5931)
                     /locus_tag="VFD06_05575"
     CDS             complement(4678..>5931)
                     /locus_tag="VFD06_05575"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZN03038.1"
                     /translation="DIHWGYGFPIGGVAAFDPDDGVVSPGGVGYDINCGVRLLRSEVR
                     ADEAAGRMRDLVEALFRNIPTGVGSHRRDFKVTQPEEKRLLAQGAAWAVKQGFGDPDD
                     LLHIEEGGALPGAEPSEVSERARERGRTQLGTLGSGNHFVEVQRVAEIHDVDAARALG
                     LEEGNLTVSIHTGSRGLGYQVCDDFLQVMMKASRRHGIHLPDPQLCCAPLGSEEGQRY
                     FGAMAAAANFAFGNRQLITHFVRKSFEETFARGPKDLGLGVVYDVCHNIAKFERHVVD
                     GRERRLCVHRKGATRAFPPQHPSTPAAYREIGQPVLIPGDMGRASWVLIGTGKGYEET
                     FGSSCHGAGRVMSRHAARKAARGRSIAREMEDRGVVVRSDGRETLAEEMPEAYKDVTD
                     VVNVVHRAGLSRKVAKLTPVGVIKG"
BASE COUNT          876 a         2226 c         2018 g          811 t
ORIGIN      
        1 agccgcaatc cggtcttcat cctggacgag gtcgacaagc tggggatgga cttccgcggc
       61 gacccggcgt cggcgctgct cgaggtcctc gacccggagc agaaccgcaa cttccaggac
      121 cactacctcg acgtgccctt cgatctgtcg gcggtgctgt tcatcaccac cgccaacatc
      181 ctcgacacca ttccggggcc gctgcgcgac cggatggagg tgctcgagct gccgggctac
      241 acggaggagg acaagctggc gatcgcccgc ctcttcctga tcccgcgcca gacgacggag
      301 aacggcatca cggtggacga catctccctg agcgacgaga cgctgcgtgc ggtgatctcg
      361 ggctacacgc gcgaggcggg gctgcgcaac ctcgagcgct ccatcggcac gctgtgccgc
      421 aaggtggcgc gcgggcgcgc cgagggagag acaggcctcg tcgtcatcac ccccgacgat
      481 ctgttctcgt acctcggccc ggagcgcttc acgagcgagc tggcggagcg gatcgcccag
      541 cccggcgtgg cggtggggct cgcctggacg ccgttcggcg gcgagatcct gttcgtcgag
      601 gcgacccgga tgcccggctc gaaggggctc atcatcaccg gacagctggg cgacgtgatg
      661 cgcgagtcgg cgatggcggc actctcgttc gtgaggagcc gggcgaagtc gctcggcatc
      721 gacccggact tcttttcgca gtacgacctg cacgtgcacg tcccggccgg ggcgatcccg
      781 aaggacgggc cgtcggccgg cgtgaccatg gcgaccgccc tggcctcgct cctgaccggg
      841 cgtccggtgc ggggcgagac ggcgatgacc ggcgagatca ccctgcgcgg caaggtgctg
      901 ccggtcggcg gcatcaagga gaaggtgctg gcggcgaagc gggccggcat ccggacgatc
      961 ctcgtgcccg ccgacaaccg caaagacctg tccgacgtcc cgaaggaggc gctcgacgcg
     1021 ctcaccttcc acttcgtctc gacggtggac gaggtcctcg cgaacgccct gcagccgacg
     1081 ccggcggagg agccggcgac tccgaagacc gtcgacgagg agacgactcc ggccgacgat
     1141 gcggcaggct cctcccccga ggtcggagac aagggacgcg ccgtcgccgc cggcaagacg
     1201 tcccggccgc tcccatccgg caagcgccgc gtcccgaacc gcgggcctga cgtcactccc
     1261 tgatccggcg ccctcccgca gcccatctcc aagcgggagg cgcgggtcag ccggagagtt
     1321 cccgcaccgc cgcctcgagc cgctgggtga cctgcgtcgg atcggcgtcg gctgcgaaag
     1381 tcatcggcgc cccgtagacg atgcgggtcg ggtggaacgc cggccagtag cgctcgtccg
     1441 ggaggacgtg gtgggtgccg gagacgtgca ccggcacgac cggggcgcgc agctcgcgcg
     1501 cgaacacccc gatgccgccg cggaagcgct ccatcgtccc ctccggcacg tgccgcccct
     1561 ccgggaagat caggatggag aagccggcgt ccatcagctc gccggcgtac accacgctgc
     1621 ggcgaaaccc ggccgtctcc ggcagcggga aggcgttgaa gaaaaacgcg gtcagccccg
     1681 cctgcagccc ctcgaggagg tactgcccac gcgtgccggc ggtctcgccg aaccagacgc
     1741 ggtgatagcg cgtcgtcatg gccggcgcga tgcgaccgcg catgggacgt ggcagcgtcg
     1801 atcgcagcag cgccgtgtcg aaataggaat ggtggttggc gacgaagaga tacggcggtg
     1861 acgcggcctc gagccgctcg cgcccgcgga tgtccggccg cccccagacg tggacgatcg
     1921 gccgctgcag cgcctcttcg accaggcgac ggttgacgtg catcggccag gagcgcgccc
     1981 accgcggcat ccgcagcgcc ccgcggatca cccgggcgct ccaggacgcc gcgccgcccg
     2041 tgcccccgtc ccggttcgac ggcgcgaccg cggcgggcgc cggctcggaa tccggagccg
     2101 aagacggtcc ggcgtccgcg gtcgtcggcc gcaccgccgg cgccggcgcg gccgcgccga
     2161 actcgcgccg tcccgacagc gcggtgcggg cccgtgcctc cagctcgccc acggtcgccg
     2221 tggccatccc gtcctcggcg atcgtcaggc cgaagtcctc ctcgagcgcc gccgcgacct
     2281 ccaccaggtc gaggctcgcg aggccgagat cctcgacgag gcgcgtcccg tcttcgaggc
     2341 gttccgccgg cacccgcgcg atccgcgcga tgatcggcct caccccgccg gccgccgagg
     2401 ccctgcgctc ccctcccggt gtctccttct gccccgccat cacggaggcc cgcagcgcca
     2461 cccgcttcac cttgccggtg gaggtgcgcg ggaaatcgcc ctcggtccag acggtccagt
     2521 cccggaccct ctgcttcggg aggagccggc ggttcgcttc ggtgacgagg gccgccgcgt
     2581 ccgcccccgg cgcgaggagg agcgccgcgt gcacctgctc tcccgtcgcg tgcggcagac
     2641 cgaaggccgc ggcgtcgcgc aggccgggga ggccgaagaa ggccgcttcc acgtcggtgg
     2701 cgtagacgtt ctccccctcc ggcgtgacga tgacgtcctt gatgcgcccc ttgatggcga
     2761 tccgcccctg cgcgtcgatc tcaccgatgt cgccggtgcc gagccactcc cctccctcct
     2821 tctggccgaa gtagccggag gtgacgttgg cgccgcgcac ctggatctcg ccgtccggcc
     2881 cgagccgcac ctcctgcccc ttcagggccc ggccgacgtc gccgcggccg ccccgctgga
     2941 aggggttgga gatcgacacg atcggcgccg tctcggtgag accgtacccc tgcacgacga
     3001 ggaccccgat ccgctcgtag aactcccgca ccggatcggc gagggccgcg ccgccgctga
     3061 cgaccaccgt gaagcgccag ccgaggctcc tgcgcacctg ccggaagagg agccgctgca
     3121 gcacgaacag cttgccggcg tgttgcgcga gccgcgcgtc gaggcgggcc tgggcgtccg
     3181 cgtgccccgc ctcgcggcgg atctccgccg ccaggagctc gaggaggcgc ggcaccgaga
     3241 agagtcccca ggctccgacg cgcgtcgccg cgtcgagcac gtcgcgcggc cgcggcgcca
     3301 ccaggaccac cgtcagcccc atgtagaggg acaggaagac gttcatcgcc tgcccgaaca
     3361 tgtgcgacag aggcagcgtg ctcaggacgc ggaactcgcc gagggggacg atgattcgct
     3421 cgtaatggcg gaagatctcc tcgagcggtg cgaagtcggc cgccaggttg gcgtgggtca
     3481 gcaccacgcc cttcggctcc ccggtcgtcc cggaggtgaa gacgatctcc gccgtgtccg
     3541 tgagcgccgc ggcggccgcc gccgaagacc gcgcgccgcc cgcggatccg tcgggatgcg
     3601 cgcgtcgcca ggccccgaac tcgatgcgcc ggatgccggc aggcgccggg atcgacgccg
     3661 gcgccaggag caggcgcgcc cccaccgtcg aggcgatctt ctcccggaag ggatcggcgg
     3721 tcgccgcctc gagcggcacg gcgacgcccc cggccagaaa gattccgagg agcccctcca
     3781 cccagtccgg ctcgtccggt ccctgcacca ggaccggctc cttcgacttc aggccggcgg
     3841 cccgaagctc tgcggcgacg gcgaggactc cggcgtgcaa ctcggcgtag ctccgctcgc
     3901 gccagcggac accatcgcgc cagagatacg cgcgcctgcc cccgaagcgg cggagccgtt
     3961 cgacccgatc ggccggggtg agaggcgtcg gcgctgcggc ggaggaggac atggaaggca
     4021 gatgctaccg cgtccgcgcc gcgatgggga gggcatgctg ccccggcttc gtgtggcccg
     4081 gacccggcca ttgccatttc acggacaggc tcctagaatc cagacgatgc ctcaccattt
     4141 ctcgctcgac gatttcgtgg ccgagatggc gcacctgtcg tgcgaaaacc tgacgatcgg
     4201 catcctgcac cagctcgccg gcgatctcga cgtcagggac gacctcatcc gggagcacat
     4261 ccacttcacg aacgacgcct acacccggaa cctggtctgc cgcacgccac gcttcgacat
     4321 gctggtgctg tgctggaagc cgggccacgt gacgacgatc cacgatcacg ccgggtcgct
     4381 caacgtgacg cgcgtcctgc agggaaggct cacctcgcgc ctgttcgtcc ccggcggacg
     4441 gccgaccccc gatcggtgtc tcgtgcgcct ggcgagcgag gaccagctgg gaccggggga
     4501 tttctccctc gtggaccggg cggagatcca ccagctcgcc aacacctccg acgccgatct
     4561 cgtgaccctg cacgtgtacg cgaagccgct gcgggacatc gtcgtgtacg acccggcgac
     4621 cggcgacatg aagaaggtcg cgctgcgcta ctctctcgaa gacgactttc agtagggcta
     4681 gcccttaatc acgccgaccg gcgtcagctt cgcgactttc cgtgacagcc ccgcccggtg
     4741 caccacgttg accacgtcgg tgacgtcctt gtaggcctcc ggcatctcct cggccagcgt
     4801 ctcgcgcccg tccgatcgca ccacgacgcc gcgatcctcc atctcccgcg cgatcgatcg
     4861 gccgcgcgcc gccttcctgg cggcgtgccg gctcatcacc cggccggctc cgtggcagga
     4921 ggagccgaag gtctcctcgt accccttgcc cgtgccgatg aggacccacg acgcccgccc
     4981 catgtccccc gggatcagga ccggctggcc gatctcccgg taggcggctg gcgtggaagg
     5041 atgctgcggc gggaaggcgc gcgtcgcccc cttgcggtgc acgcacaggc gccgctcccg
     5101 gccgtcgacg acgtggcgct cgaacttggc gatgttgtgg cagacgtcgt agacgacccc
     5161 gaggccgagg tccttcggac cgcgcgcgaa cgtctcctcg aacgatttcc gcacgaaatg
     5221 cgtgatgagc tgccggttgc cgaaggcgaa attcgccgcc gccgccatcg ctccgaagta
     5281 gcgctgaccc tcctcggagc cgagcggcgc gcagcacagc tgcgggtccg ggaggtggat
     5341 cccgtgccgg cgcgacgcct tcatcatcac ctgcaggaag tcgtcgcaca cctggtaccc
     5401 gagtccgcgc gagccggtgt ggatcgagac ggtcaggttc ccctcctcga gcccgagggc
     5461 acgcgccgcg tccacgtcgt ggatctccgc cacccgctgc acctcgacga agtggttgcc
     5521 ggagccgagc gtcccgagct gcgtccggcc gcgctcgcgc gcccgctccg acacctccga
     5581 cggctccgcc cccggaagcg cccctccctc ctcgatgtgc aggaggtcgt cgggatcgcc
     5641 gaacccctgc ttcaccgccc aggccgcccc ctgcgcgagg agccgcttct cctcgggttg
     5701 agtgaccttg aaatcacgac ggtgcgagcc gacgccggtc gggatgttgc ggaacagcgc
     5761 ctcgacgaga tcgcgcatcc gccccgccgc ctcgtccgcc cgcacctccg atcgcagcag
     5821 ccgcacaccg cagttgatgt cgtagccgac cccgccgggc gaaacgaccc cgtcgtccgg
     5881 atcgaatgcc gcgaccccgc cgatcgggaa gccgtagccc cagtggatgt c
//
LOCUS       DASRIQ010000226         4861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1660390, whole genome shotgun sequence.
ACCESSION   DASRIQ010000226 DASRIQ010000000
VERSION     DASRIQ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660390"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VFD06_05580"
     CDS             complement(<1..681)
                     /locus_tag="VFD06_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN03039.1"
                     /translation="MTSPFRALLLSRLREFYREPEAIFWVYGFPVLLAIGLGIAFRER
                     PPEEVRVDVLVTRAGAAPSSDVPDPAAAAAALVALQGDARFRARTCAGEECAQRLRLG
                     RTDIVVVARADGVEFRFDPTRPESVLARARAEEALQRAAGRSDPVAIREREVKEPGSR
                     YIDFLIPGLLGMNLMGGGLWGIGFVIVDMRVRKLLKRLVATPMRRTDFLLAMMGSRIL
                     FMLPEVALL"
     gene            complement(678..1649)
                     /locus_tag="VFD06_05585"
     CDS             complement(678..1649)
                     /locus_tag="VFD06_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN03040.1"
                     /translation="MQPVKGVAIRCSGLRKRFVSRSAMVDAVDGLDLEVRSGECFGLL
                     GPNGAGKTTTIEILEGLLPPTSGEVEVLGGRWDAHPDDLRQRIGISLQETRFAEKLSV
                     RETLVLFRSFYRRGIAPEEAIALVSLQEKSGAWVGKLSGGQRQRLAVACALVGDPELL
                     FLDEPTTGLDPQSRRALWEILRELRARGRTILLTTHYMDEAERLCDRVAVVDHGRVIA
                     LGSPAELVARLGGEHVIEFALEAGPDGLREEDYRGLPGVSGARIDLEGVSLTVTEPHV
                     TLPALLERLGRERLALARLATRHVSLEDVFVDLTGRHLRDDTASSAA"
     gene            complement(1697..2377)
                     /locus_tag="VFD06_05590"
     CDS             complement(1697..2377)
                     /locus_tag="VFD06_05590"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HZN03041.1"
                     /translation="MKAGWIADATCAVLVLGMFLYGALAWPNAPDRFPMHWNLHGEVD
                     RWGGRFEGLFVLPLIALATSLLLRFLPRVDPGRANYDNFSGAWATMRVTVAAILTVVY
                     GIVQVAARGRAIEVRTVMPLAIGALFVVLGNLLGKLRPNWFFGIRTPWTLSSAESWNR
                     THRAGGWVFIGLGLLMIVSGLLAPGRSILIVVALVLLATFGLAVYSYVVWRRDPDKVP
                     PAGRTAAP"
     gene            complement(2374..2655)
                     /locus_tag="VFD06_05595"
     CDS             complement(2374..2655)
                     /locus_tag="VFD06_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN03042.1"
                     /translation="MTEVLQALADPTRREILRRLRRRDLTAGEIADGFPLAKSTLSGH
                     FNVLKRAGLVVTERRGTTIVYSLNVSALEQALAGLWDLLGRTREKEGKR"
     gene            complement(2767..4086)
                     /locus_tag="VFD06_05600"
     CDS             complement(2767..4086)
                     /locus_tag="VFD06_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HZN03043.1"
                     /translation="MRAERERFIDVMRELLPGLLFAAALGLFAFRLAIPPRYVMDEVY
                     HAFTAAQIVEGNPDVFNWNAKAPRAGVGYTWNHPPLGVLLIAGGVAIWGDGPFGWRFA
                     SALFGALGATLGYLLAWRLTGHRTAGVLTALFLMLDTLYFTQSRIGMLDIFGTVFMLA
                     AFLFLHRTLVARPAPRRADLMMTGLCLGAAIAVKWNAAYATAVIAVVLLARVARTGLS
                     RRDGSPGESGAVFRREAAWILLAVGVVPTVVYVAAYTPFFAAGSGPREFLALQEQIFR
                     YHAGLQAAHDYASRWWQWPLTLRPVWYYVQYDPDGTVAHVYANGNPLLHWLFVPAVCA
                     VLWAWRRRPPLPLILAVGFFGQWLPWALVPRVAFVYHFLPAATFGCVALGIGIAALLG
                     AGRWRRTLGIAYAVAVLAAFVFFYPTSSAVPLTRQAIEARMWLDSWR"
     gene            4257..>4861
                     /locus_tag="VFD06_05605"
     CDS             4257..>4861
                     /locus_tag="VFD06_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03044.1"
                     /translation="MAAEFLRRAQPLDEIVGRIEVRRGRMARVPVPVDCPGNRRGCLD
                     EQTFDEAVRFDFIVERRSLRDGSLDETETPAGTILVAASFSDRGRFAFWDGIEAAAQR
                     LAAEAFASPFAGWSPGQAPFDGDSVVRSADTWWGCFESSEKSVLVHFIPPARRGSAAP
                     EGTAFGTRVYGPPPDGTCAAIRAATGSAQAEAHRTAPSPLRS"
BASE COUNT          779 a         1753 c         1742 g          587 t
ORIGIN      
        1 caggagcgcc acctccggca gcatgaacag gatgcggctc cccatcatcg cgaggaggaa
       61 atcggtccgg cgcatcggcg tcgccaccag gcgcttgagg agcttgcgca ccctcatgtc
      121 cacgatgacg aagccgatcc cccagagtcc gccccccatc aggttcatgc cgaggagtcc
      181 cgggatcagg aagtcgatgt agcgcgagcc cggctccttg acctcgcgct cccggatcgc
      241 gaccggatcg ctgcgccccg ccgcccgctg cagggcctct tcggcgcgcg cccgcgccag
      301 gacgctctcg ggccgggtcg ggtcgaagcg gaactcgacg ccgtccgccc gcgccaccac
      361 cacgatgtcg gtccggccga ggcgcaggcg ctgcgcgcac tcctccccgg cgcaggtgcg
      421 cgcccggaac cgcgcgtccc cctgcagcgc cacgagcgcc gccgcggccg cggccgggtc
      481 cggcacgtcc gacgacgggg cggccccggc ccgggtgacg agcacgtcca cccgcacctc
      541 ctccggcggc cgctcgcgga aagcgatgcc gagcccgatc gccagcagca ccgggaaccc
      601 gtagacccag aagatcgcct ccggctcgcg gtagaactcg cgcagccggc tcagcagaag
      661 ggcgcggaag gggctcgtca tgcggcggac gaggcggtgt cgtcgcgcag gtggcgaccg
      721 gtcaggtcga cgaagacgtc ctcgaggctg acgtggcgcg tcgccagacg ggccagcgcc
      781 agacgctccc gtccgagccg ctcgagcagc gccgggagag tcacgtgcgg ctcggtgacg
      841 gtgagcgaga ccccctcgag atcgatgcgc gccccgctca cgccgggaag cccgcggtag
      901 tcctcctcgc gcagtccgtc cggccccgcc tcgagcgcga actcgatgac gtgctcgccg
      961 ccgagccgcg ccaccagctc cgccggcgag ccgagcgcga tgacccggcc gtggtccacg
     1021 accgccaccc ggtcgcacag gcgctccgcc tcgtccatgt aatgcgtcgt caggaggatc
     1081 gtccgtccgc gcgcccgcag ctcccgcagg atttcccaca gcgcgcggcg cgactgcgga
     1141 tcgaggccgg tggtcggctc gtcgaggaac agcagctccg gatcgccgac gagggcgcag
     1201 gcgaccgcca ggcgctgccg ctggccgccg gagagcttgc cgacccaggc gccgctcttc
     1261 tcctgcaggg agaccagcgc gatggcctcc tccggcgcga tgccgcggcg gtaaaaggag
     1321 cggaacagga cgagcgtctc gcgcacgctc agcttctcgg cgaaacgggt ctcctggagc
     1381 gagatgccga tccgctggcg caggtcgtcg gggtgcgcgt cccagcgccc gccgaggact
     1441 tcgacctcgc ccgaggtcgg cggcagcagc ccctcgagga tctcgatggt cgtcgtcttg
     1501 ccggcgccgt tcggtccgag cagaccgaag cactcgccgg agcgcacctc gaggtcgagc
     1561 ccgtccaccg cgtcgaccat ggcggagcgg ctgacgaagc gcttcctgag gccactgcag
     1621 cggatggcga cccctttgac cggctgcatg gcgggatgat acccgcgaac cgggagggtc
     1681 ccggcgtggc gggccttcag ggcgccgcgg tgcggccggc cggcgggacc ttgtcgggat
     1741 cgcggcgcca gacgacgtac gagtacacag ccaggccgaa agtcgcgagc agcaccaggg
     1801 cgaccacgat gaggatcgag cggcccggag cgaggaggcc cgagacgatc atcaggaggc
     1861 cgagcccgat gaacacccag ccgccggcgc ggtgcgtgcg attccacgac tcggcgctcg
     1921 agagcgtcca gggggtgcgg atgccgaaga accagttggg gcgcagcttg ccgagcaggt
     1981 tcccgaggac gacgaacaat gcgccgatcg ccagtggcat gacggtgcgg acctcgatgg
     2041 cccggccacg ggcggccacc tggacgatgc cgtacaccac cgtcaggatc gccgcgaccg
     2101 tcacgcgcat cgtcgcccac gccccggaga aattgtcgta gttggcccgc cccggatcga
     2161 cgcgcggcag gaagcgcagc agcagcgacg ttgcgagcgc gatgagcggc agcacgaaca
     2221 gcccctcgaa gcgcccaccc cagcgatcca cctccccatg caggttccag tgcatcggaa
     2281 agcggtcggg agcgttgggc caggccagcg ccccgtacag gaacatgccg agaaccagga
     2341 cggcgcacgt cgcatcggcg atccacccgg ctttcatcgc ttcccctcct tctcccgcgt
     2401 ccggccgagg agatcccaca ggccggcgag cgcctgctcc agggccgaga cgttcaggct
     2461 gtagacgatg gtcgtgccgc ggcgttccgt gaccacgagc cccgcccgct tcagaacgtt
     2521 gaagtggccg gagagggtcg atttcgccag cgggaacccg tcggcgatct cccccgcggt
     2581 caggtcacgc cggcgcagcc gccggaggat ctcccggcgg gtcggatcgg cgagtgcttg
     2641 cagcacctca gtcatttcgt tatttcgcga acgaccgaaa tatagtcgca tcaggcggcc
     2701 aggtcaagag cgtcgcgacg ggaggtgcgg aaggccggtg ggcggggtgc cagggagcct
     2761 gggagcctag cgccaggagt cgagccacat gcgcgcttcg atcgcctggc gcgtcagcgg
     2821 cacggcgctg ctcgtcgggt agaagaagac gaacgccgcg aggacggcga ccgcgtaggc
     2881 gatcccgagc gtccgccgcc atcgcccggc ccccaagagc gcggcgatgc cgatcccgag
     2941 cgccacgcac ccgaacgtcg cggcgggtag gaagtggtag acgaaggcca cgcgcggcac
     3001 gagcgcccag ggaagccact gcccgaagaa cccgacggcg aggatcaggg ggaggggcgg
     3061 ccggcggcgc caggcccaca gcacggcgca gactgccggc acgaacagcc agtggaggag
     3121 cgggttgccg ttggcgtaga cgtgcgccac cgtcccgtcc ggatcgtact ggacgtagta
     3181 ccagaccggc cgcagggtga gcggccattg ccaccaccgc gacgcgtagt cgtgcgccgc
     3241 ctgcagcccg gcgtgatagc ggaagatctg ctcctgcagc gccaggaact cgcgcggccc
     3301 gctgccggcc gcgaagaacg gcgtgtaggc ggcgacgtag accacggtcg gaacgacgcc
     3361 gacggccagg aggatccagg ccgcttcgcg ccggaagacc gcgccggatt cgcccggcga
     3421 cccgtccctc cgggacaacc cggtgcgggc gacgcgcgcc aggagcacga ccgcgatcac
     3481 cgccgtggcg taggcggcgt tccatttcac cgcgatcgcc gcgcccaggc agagaccggt
     3541 catcatgaga tccgcgcggc gcggcgccgg ccgggcgacg agcgtccggt gcaggaacag
     3601 gaacgccgcg agcatgaaga cggtcccgaa gatgtcgagc atcccgatgc gcgactgggt
     3661 gaagtacaga gtgtcgagca tcaggaaaag cgccgtcagc acgcccgcgg tccggtgccc
     3721 cgtcagccgc cacgccagga ggtagccgag cgtcgccccg agcgcgccga acagggcgct
     3781 cgcgaaccgc catccgaacg gaccgtcccc ccagatggcg acgccccccg cgatgaggag
     3841 cacccccagg gggggatggt tccaggtgta gccgacgccg gcgcgcggtg ccttcgcgtt
     3901 ccagttgaag acgtccgggt tcccctcgac gatctgggcc gccgtgaacg cgtggtagac
     3961 ctcgtccatg acgtagcggg gagggatcgc gagccggaag gcgaagagtc ccagagccgc
     4021 cgcgaacagg agccccggca acagctcgcg catcacgtcg atgaagcgct cccgctccgc
     4081 ccgcatcccc cctcgtcccg gcgcgcgccc gacccatggg gagccatggc gccgccgccg
     4141 gcggggacgt atcttaggtc gcacccgggg gaatcgccat gcggtgcgga acagcaggac
     4201 gcggtcggcg tgccgtcgcg atcgcggcgc tcgcttccgc cgtcacgtcg agcggcatgg
     4261 cggccgagtt cctgaggcgg gcgcagccgc tcgacgagat cgtcggacgg atcgaggtgc
     4321 ggcgcggccg gatggcgcgc gtgccggttc cggtcgactg tcccggcaac cgccgcggct
     4381 gcctggacga gcagaccttc gacgaggcgg tccgcttcga cttcatcgtg gagcggcgct
     4441 cgctgcgcga cgggagcctg gacgagaccg agacaccggc gggcaccatc ctggtcgccg
     4501 cctcgttctc cgaccgcggc cgattcgcct tctgggacgg catcgaagcg gcggcgcagc
     4561 ggctggcggc ggaagcgttc gcctcaccgt tcgccggctg gagtcccggg caggccccct
     4621 tcgacggcga ctccgtggtc cgctcggcgg acacctggtg gggctgtttc gaatcctcgg
     4681 agaaaagcgt cctcgttcat ttcatccctc cggcacggcg aggcagcgcc gctccggagg
     4741 ggacggcgtt cggaacccgg gtgtacggac cgcctccgga cgggacgtgc gcggcgattc
     4801 gagcggcgac cggttccgcc caggcggagg cgcaccgcac ggccccctcg cccctccggt
     4861 c
//
LOCUS       DASRIQ010000227         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3215019, whole genome shotgun sequence.
ACCESSION   DASRIQ010000227 DASRIQ010000000
VERSION     DASRIQ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3215019"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFD06_05610"
     CDS             <1..544
                     /locus_tag="VFD06_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03045.1"
                     /translation="SMALLSRMALATVLLLIVVACNVEDGPDMVPVPVLDFTPTDFNG
                     RWKMTRHLVSDDCAGNYVFSSVDSGFLDITETGSIFIVENLNECAAPVYAGLGTANGD
                     VATVVSDYNTSTNPQCIVTEHWIETVSRSGSTLSGDIVVTVTNSPDCNSGPTPCNVRM
                     TLEAESCISVICFSYPPSCP"
     gene            592..849
                     /locus_tag="VFD06_05615"
     CDS             592..849
                     /locus_tag="VFD06_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03046.1"
                     /translation="MHRGSRNLVVGLLALAATGVLADGASIPSGAPAPAPVQVEVPTE
                     CLAERNHGACVNCCKDKTGLDGNICTRFCRNVVPPLPEPQP"
     gene            1046..>2804
                     /locus_tag="VFD06_05620"
     CDS             1046..>2804
                     /locus_tag="VFD06_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN03047.1"
                     /translation="MTALVLASLALAAVTASSAAPTRFSSVSARHGPGGPRYVEGEIL
                     VKFRCDAHVASRGPVRADVAGSTQRRFASGAELWRLGAGVAVQDALQRVKGMPGIEYA
                     EPNWLLHATRIPNDPFFPQQYGLANTGQEGGEAGADIDAAGAWDISTGGASVIVAVVD
                     TGIDLDHPDLVPNLWTNPDEIPGNLVDDDGNGRIDDVHGWDFVNDDGDPRDDEGHGTH
                     VAGTIAAAGDNDLGVAGVAWRARLMALKILDASGTGTTSDAIQAIDYAAARGARILNA
                     SWGGGGFSMAMLESIRDAGAHEVLFVAAAGNDAADSDLVPFSPAGYDAPNVVAVAATD
                     RHDHLAPFSNFGGTKVDLAAPGVDILSTLPGGTYGLSSGTSMATPHVSGIAALILGFA
                     PGMQTASLRRRILENAEPVASLADKVATGARVNAFQCLLRSETVPPGPIVDLRVTEPL
                     SDGLALAWTAPGDDLDSGAAFTYDLRVSGAPFAAAGFETAPRFPLSGPPRSAGSPETM
                     EIGGLEPSRTYFLGLRAIDEWGNAGPPGFATATTRPPPAGQVTPESFSASLRSGAVMT
                     TLLTMRNTGEGTLDWSAARP"
BASE COUNT          426 a          985 c          933 g          460 t
ORIGIN      
        1 tagcatggca ctcctatccc gcatggcgct cgcgaccgtt ctgctcttga tcgttgtcgc
       61 gtgcaacgtg gaggatgggc cagacatggt gcccgtgccg gtgctcgatt tcactcccac
      121 agacttcaat ggcaggtgga agatgaccag acacctcgtc agtgatgact gcgcgggcaa
      181 ctatgtgttt tcgtcggtgg acagcggctt cctggacatc acagagacgg ggagcatttt
      241 catagtggag aacctgaatg agtgcgccgc gcctgtctat gcgggcctcg gaacggcgaa
      301 cggggatgtc gccactgtcg tctcggacta caacacttct acgaaccctc aatgcatcgt
      361 gaccgagcat tggatcgaga ctgtttcccg tagcggctcc actctaagcg gtgacattgt
      421 ggtcacggtc acgaattcgc ccgattgcaa ttcggggccg acaccctgca acgtcaggat
      481 gacattggag gcggaatcgt gtatttccgt aatctgcttt tcctatccac cttcctgtcc
      541 ctagtcatcg atcaccgctc ggagaatcgc acgcacagag gaggagagtc catgcatcgt
      601 ggatcccgga atctcgtcgt cggcctgctc gctctcgccg ccaccggcgt tctcgcggat
      661 ggggcctcca tcccctccgg cgcgcctgcg cccgctcccg ttcaggtcga ggtgccgacg
      721 gagtgcctgg ccgagcggaa tcacggcgcc tgcgtgaact gctgcaagga caagacgggc
      781 cttgacggca atatctgcac ccgcttctgc cggaatgtcg tcccgccgct gccggagccg
      841 cagccgtaga cgaagcgctg cgagccggcc gcggtgttct gagtccgatc acaatccgag
      901 cccgcgcggc gcacgaaccg catcattccg gatccaggga gtgattgcgt ctgcctgcgg
      961 cgagataagg taacagcacg ttttcggctc gctcacgagg gttcggggaa tgcgtcgtcc
     1021 gtccaggccg ggatgcgccc gacatgtgac agcgctcgtc ctcgcctcgc tcgccctggc
     1081 cgccgtcacc gcgtcctccg ccgctcccac gcgcttctcc tccgtgtccg cccgacacgg
     1141 cccgggcggt ccgcggtatg tcgaaggtga gatcctcgtc aagttcaggt gcgatgcgca
     1201 cgtcgcgtcg cgcgggcccg tgcgcgcgga tgtggccggg tcgacgcagc gccgcttcgc
     1261 gtccggcgcc gagttgtggc gtctcggcgc cggcgtggcg gtccaggacg cgctgcagcg
     1321 cgtcaaagga atgcccggga tcgagtacgc cgagccgaac tggctcctgc acgcgacgcg
     1381 catcccgaac gatcccttct tcccgcagca gtacggcctc gcgaacaccg gccaggaggg
     1441 gggcgaggcg ggggccgaca tcgatgccgc cggggcgtgg gacatctcca ccgggggcgc
     1501 gtcggtcatc gtggcggtcg tggacactgg tatcgacctc gatcatccgg acctcgtccc
     1561 caacctgtgg accaacccgg acgagatccc ggggaatctc gtcgatgacg acggcaacgg
     1621 ccggatcgac gatgtccacg gctgggactt cgtcaacgac gatggcgacc cgcgcgacga
     1681 cgaagggcac gggacgcacg tcgccgggac gatcgccgcc gccggggaca atgacctcgg
     1741 ggtcgcaggg gtcgcctggc gcgcgcgcct gatggccctg aagatcctcg acgcgagcgg
     1801 caccggcacg acctcggacg ccatccaggc gatcgactac gccgccgcca ggggggcgcg
     1861 catcctcaat gcctcgtggg gcggcggcgg cttctcgatg gccatgctgg agtcgatccg
     1921 cgacgccggg gcgcacgagg tgctgttcgt ggcggcggcc ggcaacgacg cggccgactc
     1981 ggatctcgtc cccttctccc cggccggcta cgacgccccc aacgtcgtcg ccgtcgccgc
     2041 gacggatcgc cacgaccatc tcgcaccctt ctcgaacttc ggcgggacca aggtcgatct
     2101 cgcggcgccg ggtgtcgaca tcctctcgac cctgccggga gggacgtacg gtctgagcag
     2161 cggcacgtcg atggcgacgc cgcacgtctc cgggatcgcg gcgctcatcc tcgggttcgc
     2221 cccgggaatg cagaccgcat cgctgcgccg gcggatcctt gaaaatgccg agccggtcgc
     2281 ctcgctcgcg gacaaggtcg ccacgggcgc gcgggtgaac gccttccagt gcctgctgcg
     2341 ctccgaaacg gtcccgccgg gccccatcgt cgatctccgg gtgacggagc cgctgagcga
     2401 cggtctggcg ctcgcctgga ccgccccggg cgacgacctc gacagcggcg ccgccttcac
     2461 ttacgacctg agggtttccg gggcaccgtt cgccgccgcc gggttcgaga ccgccccgcg
     2521 cttcccgttg tccggcccgc cccgctccgc cggctcgccg gagacgatgg agatcggcgg
     2581 cctcgagccc tcccggacct acttcctcgg cctccgggcg atcgacgaat gggggaacgc
     2641 cgggccgccg ggcttcgcga ccgcgacgac ccggccgccg ccggccgggc aggtgacgcc
     2701 ggagtccttc tccgcgtcgc tgcgcagcgg tgcggtgatg accaccctcc tcacgatgcg
     2761 caacaccggc gagggcacgc tcgactggag cgcggcgagg ccga
//
LOCUS       DASRIQ010000228         4849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1149581, whole genome shotgun sequence.
ACCESSION   DASRIQ010000228 DASRIQ010000000
VERSION     DASRIQ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149581"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(520..2724)
                     /locus_tag="VFD06_05625"
     CDS             complement(520..2724)
                     /locus_tag="VFD06_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN03048.1"
                     /translation="MGVRETEPPPLRVARRSRAAGRPRMVPGVRCGSRMPVVDTGTPW
                     ENPLKLIFAVGFACGCAGLSSMPLVAEAVPVRHSVMFQGKVGGRQTTQRGTDGRVTVD
                     LSYRDNGRGPDYHEEIRLDDGGVQLAHTITGKSTFGAPADDEFSRAGGVARWKSVSDS
                     GSASTKAAAFYVPVNTAFETYAMLARAALRQPDGRMTLLPSGEARAIRVVSREVESSG
                     RRQKLALVALKGLDLQPTYVWLTDDVEQRFFAHVFPGWIQVIEEGWEATAPAIEAAQV
                     AAEKEWLEQMAARLTHRLSDPILIRNARVFDSEKAILGPPSDVYVFNGRVAAVWPVDT
                     GTPDAATQIDAGGKTLLPGLFDMHDHSSAWGTPLQIAGGITTARDKGNDNPTLFDLID
                     KCDSLRHVGPRIEPSGYIEGDSEFASRSGIVVADLDAARRAVDWYARRGYREIKLYNS
                     IKPEWVAPIAAHAHDRGLRVTGHIPAFMSAEQAVRAGYDEVTHINQVMLTSLAGPKDD
                     SRTLARFYLIAENADKLDLDSRPVEDFIALLKEKGTVVDPTLAVFQDMGQRHGELGKN
                     YAVVASHFPISQQRSLKRNPMNITDENQERWRAADRKLVEMVGRLHRAGIPLVAGTDA
                     IAGFTLHRELELYVEAGIPAPEALQIATWNGAKYTGTLSERGSVTPGKRADLVLVDGD
                     PTSDISAIRRPVLTMKGGVAYYPSEIYEALGITPFVEPVRAQAVSARAGGGR"
     gene            complement(2802..3062)
                     /locus_tag="VFD06_05630"
     CDS             complement(2802..3062)
                     /locus_tag="VFD06_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03049.1"
                     /translation="MRSPPPLKPCPALTLKGGTPTRGLAAPDGGGSYLPTLLVNVSAL
                     ADSLLDAHPPPTRAMPAHPRAIPIIGATTARLLLDPRMLIIE"
     gene            3022..3813
                     /locus_tag="VFD06_05635"
     CDS             3022..3813
                     /locus_tag="VFD06_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HZN03050.1"
                     /translation="MSAGQGFSGGGDLNDLAADQMTTLLDQSERFSVIERAQLQKLLD
                     EQNLEGIVVPGEMAKPAQVRGVDYLLLGKVTNLRAKKETSSTGFGLAQVGGVFNVGGA
                     DVKQKEVVITTEAGVDIRLVDPTTGEIAVSNFSEFKRTDSAGAFGLTILGASANADAD
                     LQISEDDKGKLLRLALDDALRKSLPKIDKLVKSDKVKSAPAAAPQTLASPAAATPDPV
                     APAAAAAGASATPEAPAVTKKFCGQCGKEAAAAAKFCGGCGAKLD"
     gene            3915..>4849
                     /locus_tag="VFD06_05640"
     CDS             3915..>4849
                     /locus_tag="VFD06_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03051.1"
                     /translation="MVRNASALLLIPVLARAAEPGPEEAAFRKRAAERYLAFAEATRE
                     RLLVPASCPFSPAAIVLAVGSDPAQLASFVRARIAYEPYAGVVRGAEGTLAAQAGGDW
                     DRAVLLQALLAEAGYLSRLRVVRRTEAEAAAVVDDFLRRPAAIASWYAGVNGAIEIGD
                     PAPILQEFGVEPENRRFHASAEVMRVRRILDECLDAAAVELPHLSTALGATKVGQPLD
                     SWRARLLAGAAERVEVEVEGPGVLAIAPDAAPIAGGAATLDAPPGDRIAKLSIRLAFT
                     PVTDGKPAEPVVLLDRELPLGSLFRKPIRLEIVPS"
BASE COUNT          741 a         1678 c         1650 g          780 t
ORIGIN      
        1 gtgaggagtt catccgtcgc gtcggtttcc actcattgga gcaagactgc cggattcgtg
       61 gggaggagcg tgtctgggta cttcttcctc agcacctccg cccaggaccg ccattcggcc
      121 ctcaggtact tggcggccgg acagaagatg ttgttttcgt agtgccggtg ctggcactcg
      181 atcgtgagca gcttttccag caggtctttc tgtggctgct tcaacgaagc cccatgtgcc
      241 ggtgggcacg tacgatcccg cgactcgctc cgtgatgacc ccacaacctc gcctccaccg
      301 ccacgtcgac gggatccccg catggggacg tgggtcctcc ggggacggac cgcaaaactc
      361 ctgggaagaa ctcgaagctc agagctggaa aacgtgaacg cgtacaaact tccttggagt
      421 aacttcttca gcctacagaa ggcggcacca agcactcctt cagcggctgg tctcaggagg
      481 ataaggtcct ccccgacaat aaacgagaca tttcctccat caacggcctc caccggcccg
      541 agccgatacc gcctgagcgc gcaccggctc gacgaaaggc gtgatgccga gcgcctcgta
      601 gatctccgat ggatagtagg cgacgccgcc cttcatggtc agcacggggc ggcggatcgc
      661 ggagatgtcg ctcgtcgggt cgccatccac caggaccagg tccgcccgct tgccgggcgt
      721 gaccgagccc cgctccgata gcgtccccgt gtacttggcg ccgttccacg tcgcgatctg
      781 gagcgcttcg ggtgcgggaa taccggcctc gacgtacagc tccagctcgc ggtgcagggt
      841 gaaccccgcg atggcgtccg tgccggcgac cagcggaatg ccggctcgat ggagccgtcc
      901 caccatctcg accagcttgc ggtcggcggc gcgccagcgc tcctggtttt cgtccgtgat
      961 gttcatgggg ttccttttca gcgagcgctg ctggctgatc gggaagtggc tcgcgaccac
     1021 ggcgtagttc ttgcccagct cgccgtggcg ctggcccatg tcctggaaga cggcgagggt
     1081 cgggtcgacc acggtgccct tctccttcaa gagtgcgatg aagtcctcta ccggccggga
     1141 gtcgagatcg agcttgtcgg cgttctcggc gatcaggtag aagcgcgcca gcgtgcgcga
     1201 gtcgtccttg ggaccggcga gggaggtgag catcacctgg ttgatgtgtg tgacctcgtc
     1261 gtagccggcg cgcaccgctt gctcggcact catgaaggcc gggatgtggc cggtgacgcg
     1321 caggccgcgg tcatgggcgt gggcggcgat cggcgccacc cactccggct tgatcgagtt
     1381 gtagagcttg atctcacggt agccgcgccg cgcataccag tcgacggcgc ggcgcgcggc
     1441 atcgaggtcc gcgacgacga tgccgctgcg ggacgcgaac tcgctgtcgc cctcgatgta
     1501 gccgctgggc tcgatccgcg gccccacatg gcgcaagctg tcgcacttgt cgatcaggtc
     1561 gaacagggtc ggattgtcgt tccccttgtc gcgcgcggtc gtgattccgc ccgcgatctg
     1621 taacggcgtc ccccaggcgc tcgagtgatc gtgcatgtcg aacagcccgg gcaggagcgt
     1681 cttgccgccg gcgtcgatct gggtggcggc atccggcgtg cccgtgtcga cgggccagac
     1741 cgcagcgacc cgtccgttga acacgtagac gtcgctcggc ggacccagga tggccttctc
     1801 cgaatcgaac acacgcgcgt tgcggatcag gatcggatca gacaggcggt gcgtcagccg
     1861 tgccgccatc tgctccagcc actccttctc cgctgcgacc tgagccgcct cgatggccgg
     1921 ggccgtcgct tcccaaccct cttcgatgac ctggatccag ccggggaaga cgtgggcaaa
     1981 gaaccgctgc tcgacgtcgt cggtcagcca gacgtaggtc ggctggaggt cgagtccctt
     2041 cagtgcgacg agggcgagct tctggcggcg cccgcttgat tccacttccc gggaaacgac
     2101 gcggatcgcc cgggcttcgc cgctgggcaa cagggtcatc cggccgtccg gctggcgcag
     2161 ggcggcacgc gccagcatgg cgtaggtctc gaacgcggtg ttgaccggca cgtagaaggc
     2221 cgccgccttc gtcgaggccg atccagagtc ggaaaccgac ttccatcgcg ccacgcctcc
     2281 ggcgcgcgag aactcgtcgt ccgccggcgc accaaacgtg gacttcccgg tgatcgtgtg
     2341 ggcgagctgg acgccgccgt cgtcgaggcg gatctcctcg tgataatcgg ggccgcggcc
     2401 gttgtcccgg tagctcaagt ccaccgtcac ccggccgtcg gtaccccgct gcgtcgtctg
     2461 acggcccccg accttcccct ggaacatcac gctgtgccgg acgggcacgg cctcggccac
     2521 gagcggcatc gacgacaggc ccgcgcagcc gcaggcgaaa ccgaccgcga agatgagctt
     2581 caagggattc tcccagggtg tgccggtatc gacgacaggc attctcgacc cacatctaac
     2641 ccctggcacc attctgggcc tacccgcggc cctcgaacgc cgcgcaacgc gcagcggagg
     2701 cggttccgtt tcccggaccc ccacgctaca atcccgcgct tactggcgga cggagtggcg
     2761 cacggggcgc cgcggtcgtt cgtctcggga ccgcgggggg atcattcgat gatgagcatt
     2821 cggggatcga ggaggagccg cgcggtcgtc gcgccgatga tgggaatcgc tctcgggtgc
     2881 gccggcattg ccctcgtcgg gggcggatgc gcatcgagca gcgagtccgc gagcgccgac
     2941 acgttgacca gcaacgtcgg caggtacgat cctccgccgt cgggcgcggc caggccccgg
     3001 gtcggcgtgc cgcccttcag cgtgagcgcc ggacagggtt tcagcggcgg cggcgacctc
     3061 aacgatcttg ccgccgacca gatgacgacc ctcctcgacc agagcgagcg cttcagcgtc
     3121 atcgagcgcg cccagctcca gaagctgctc gacgagcaga acctggaagg gatcgtggtc
     3181 cccggcgaga tggccaagcc cgcgcaggtc cgcggtgtcg actacctcct cctcggcaag
     3241 gtcacgaacc tccgcgccaa gaaggagacc tcgtccaccg gcttcgggct cgcgcaggtg
     3301 ggcggcgtgt tcaacgtcgg cggggcggac gtgaagcaga aggaagtcgt catcacgacc
     3361 gaggccggcg tcgacatccg cctcgtcgac ccgaccaccg gcgaaatcgc ggtcagtaac
     3421 ttcagcgagt tcaagaggac cgactccgcc ggcgcgttcg ggctcaccat cctgggggcc
     3481 agcgccaatg ccgacgccga cctccagatc agcgaggacg acaaggggaa gctcctgcgg
     3541 ctggccctgg acgatgcgct gcggaagagc cttccgaaga tcgacaagct cgtgaagagc
     3601 gacaaggtaa agagcgcgcc ggccgccgcc ccgcagaccc tggcctcgcc tgcggcggcg
     3661 actccggatc ccgtcgcgcc ggcggctgcc gccgccgggg cctccgccac gcccgaagcc
     3721 cccgcggtga cgaagaagtt ctgcgggcag tgcggcaagg aagccgcggc cgctgcgaag
     3781 ttctgcgggg gctgcggcgc caagctcgat tagccgatcc ttgccggaag agcgaggctc
     3841 gagcggtcct gtcctctccg gcagagggga gaggaccgct ttcttgatgg gctgcccccg
     3901 gttccggagt cctcatggtg cgtaacgctt ccgccctgct cctgattccc gtcctcgcgc
     3961 gcgcggccga gcccggcccg gaggaggccg cgttccggaa gcgagccgcc gagcgctacc
     4021 tggccttcgc ggaggcgacg cgggagcgcc tgctcgtccc cgcgagctgc ccgttctcgc
     4081 cggcggcgat cgtgctcgcg gtcggctcgg atccggcgca gctggcctcg ttcgtgcgcg
     4141 cgcgcatcgc ctacgagccc tacgcggggg tcgtccgcgg cgccgaggga acgctcgcgg
     4201 cgcaggcggg cggcgactgg gaccgcgcgg tgctcctcca ggcactcctc gcggaagccg
     4261 gctacctgtc ccgcttgagg gtcgtgcggc ggaccgaggc cgaggcggcc gcggtcgtcg
     4321 atgacttcct ccggcgcccg gcggcgatcg cgtcctggta cgccggcgtg aacggcgcga
     4381 tcgagatcgg cgacccggct cccatcctcc aggagttcgg cgtcgagccg gagaaccgca
     4441 ggttccacgc gtcggcggag gtgatgcgcg tccgtcgcat tctggacgag tgtctcgatg
     4501 ccgccgcggt ggagctgccg cacctctcga ccgctctcgg cgcgacgaaa gtgggccagc
     4561 ccctcgactc gtggcgggcc cggctcctcg ccggcgcggc cgagcgcgtc gaagtggagg
     4621 tcgagggacc gggagtgctc gccatcgcac cggacgccgc gccgatcgcg ggcggcgcgg
     4681 cgaccctgga cgcacctccc ggggaccgca tcgcgaagct ctccatccgg ctcgccttca
     4741 ctcccgtcac ggatggaaag cctgccgaac cggtcgtgct cctcgaccgc gagctccccc
     4801 tgggctcgtt attccgcaaa cccattcgtc tcgagatcgt cccgagcga
//
LOCUS       DASRIQ010000229        10889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2721326, whole genome shotgun sequence.
ACCESSION   DASRIQ010000229 DASRIQ010000000
VERSION     DASRIQ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721326"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="VFD06_05645"
     CDS             complement(<1..1417)
                     /locus_tag="VFD06_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03052.1"
                     /translation="MRRRTLVASASVAAALLASTAVLLALGNVEVITQNGTRIGNRVW
                     HVNSMPIVWRYHDPTTFAGCYSSPNAPPETLLPEIQTAFDAWQDDPDSRIEFVYGGET
                     SVRDQGADGVNVVTFCDSGVLASNLGFGAQVISTALTLPVVVSQGGGCPPGQGPILAG
                     ICFPAGSYPAGTLIDADIRFNSFGTVEETLATNGAVGGLHPSTQLYDVRNLATHEVGH
                     VAGLAHDPLPQATMHFFVDPNPPSDGVGERALSRSDRSTLGRYYPEASYGTAYGSITG
                     RITINNVAADGVHVVAIDPVTMLGVAGRLSLSRFEDIAALGPEGPDATFQGNGFYRID
                     GLPPGNYYVYAEYFDSSDLFPGLLPGRLSQKYNLTVGNSNVAPGSGSAGVLGFLPALA
                     EFYDAAESPWGGDGVSPGTASDNSDAASLVVVTAGAVTPNINIGINIEPPNGQTPAQR
                     QNPTTRSTLVDDNHQPSDLLWN"
     gene            complement(1414..2040)
                     /locus_tag="VFD06_05650"
     CDS             complement(1414..2040)
                     /locus_tag="VFD06_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03053.1"
                     /translation="MNDFMRSGTARIGLAVAVACSFTLRAEATSLRLLDLPTMVQSAK
                     RVVHARASATRVYWDEQRRGIFTDTDFEVLEEAKGKGPARLTVTMLGGRIDPIEMRTE
                     GTPEFEIGEEVLLMTTPRPDGRQNIVGFSQGVFRVHRNPLSGERFVTVAGSVEDPGTG
                     QPAAQAGRSPRPLATFLEQIRILASGAGAPPESPRMRLVPVPRGEVRR"
     gene            complement(2053..2637)
                     /locus_tag="VFD06_05655"
     CDS             complement(2053..2637)
                     /locus_tag="VFD06_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03054.1"
                     /translation="MTRIHRFVATLALAAGLAVPVVARANDHVRVPEDIDPNFYTSAP
                     LESLTDGEWTAIPFFRPPECIRADFNILRYFDAAALLCPLLVEGFAEFNDVGPILSSL
                     HGRGAVPIWFVRAAELAAATADRVVTIGELGALPSLQKGVAGFYNEQNHIFGVHQVSH
                     FTLVARGALDDGRAFEVGVIEVDLELVRAIIEFE"
     gene            2768..5821
                     /locus_tag="VFD06_05660"
     CDS             2768..5821
                     /locus_tag="VFD06_05660"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN03055.1"
                     /translation="MPDPRQKRVKELFQAAADLRPADRHAFLEAQCGADDALRTAVES
                     LLAAGEDKDVLLDRRPFLSAAGSGTGSPDSASPTASLGTAVGPSLVIDRYRLLEKLGE
                     GGMGEVWLAEQQAPVVRRVALKLIKAGMDTAQVIARFEAERQALALMDHPAIARVFDA
                     GATPEGRPYFVMEPVTGLPITQHSDRERLDIAARLELFMQVCEGVQHAHQKAILHRDL
                     KPSNVLVTAQDGRAAPKIIDFGVAKAMAQPLTAHTLYTGLGILGTPGYMSPEQADLGR
                     QDVDTRTDVYSLGVMLYELLVGVLPHEPERLRRAGWEGLGRLLRDEEPATPSKRLTTL
                     PADTLDVSARLRRLDVPALRRHVSGDLDWITMKAIEPDRERRYGSPADLAEDIRRHLR
                     DEPVLAAAPTAAYRLGKFVRRHRFGVAAAAAIGLVVAAGLVGTTTGLVRARRAESEAR
                     KQAAASERVAGFLSSVLASVDPARMGTTLVDDLRRRVEKVERGRGGSETQVAATLAAF
                     DGATKGINRVDAARDLVDQEILSRAGHSIDEQMRSDPLLAARLQSTLGSTYYDLGLFE
                     KAAVYHLKALETRRRELGLEHPDTLRSMDALGRSLHVSGRIDEAETTLRQAIDIQSRV
                     LGAEHPDTLQSMNDLAYAMSFAGKNKESEALYRKTLESRRRVLGNESPDAALTMRLLA
                     DNQVLQGRFAEAEQLYREALAIQRRVLGPEVHETLRSMGNMARALTGLGRHDEAERVS
                     LEVIDIRRRTSGEDSIYTVLSLGYLGMGYANQGRRQDAERVLMEALERSRRSEVSLVT
                     ALINYTLAPILRSQGRLPEAETLYRETVEIQRGTGSGRAVAGTLRLLAEVLVQEDRCQ
                     EAEPVYREIVTIQEREFGADASPTIAAKWDLARLYWGTVDVERGRSNVASFLADLERL
                     AKQPEAKADFLNLYAGCLLICEPVDVRDPQRAFPFAEKAVAASGRKDANILDTLALAY
                     HLTGRDSEALKIQREAITMLPSGPSRLREELERNLATYEQALRRR"
     gene            complement(5861..9010)
                     /locus_tag="VFD06_05665"
     CDS             complement(5861..9010)
                     /locus_tag="VFD06_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN03056.1"
                     /translation="MLAAGLAAAEVVSAQQPTTGSFVASVTDQTGAPVAEAKLTVAGP
                     LGQRVVTADDNGRVEIRLLPAGDYRVQVEKPGFGTIVIEEVTVAAGERTWLPIVLTTG
                     YTEEIEVTGAAPLVDMKRTEVVTTFKTDEVISTLPVGREFTDLARLAPGVVPGHGTGE
                     LNHSIGGSSGLENAYIIDGTNITSVGFGSVGTGLFGRTISTGIGLDFLEELQVKTAGF
                     EAEYGQALGGVINGVVKSGGNAFTGSIRFFFQPADLEAPGEEIVIEGGATQVEDRERT
                     DVSVQAGGPFIKDKLFWFVALNPVHDSSLVFRDAPTTTNPVLAFDPGAAETYPYAELQ
                     PPEALDGRRDDQRRFNYAAKVTWQVSPRHRLELSGFGDESRGDGSLGIGAGNPFNLAG
                     DLDGDGTLDVTKTAAGYLDGALRTDAEYGSGHQALRYSAVFGPNWFFESQISHRHSRF
                     EATSVVDDYPYTDERIIRGAGIDFIPRGGGGLPASSDEEAWDAALKFTGILGRHEVKA
                     GYEYWDAEYEQILGGKHVDLPFPIDTNADGVVDSTTVIRSPTGTMVTVIGGIPGCTAC
                     SSQIPSYESFTFLNPSGPTNSQEHSLFVQDTWSIGRRWVAKLGARATRQSLEGSGDFT
                     LNLSFVAPDVFTTDPTRFEPVSYTFETEISPRLGLTFDPRADGRTKLYVNAARYFERV
                     PLDLAAHAFSNDIIVLNFPFRDPDLTVRNPESSILMTALLPTRVEKNTRLPYVDEFVV
                     GWQQLLSADLMFEIRAILRDQGRVLEDVQSTSAEEFLNFSLGPDAGGNLPFPGFGSAP
                     LGQYVVTNPGRNTPKDVAYPFADPEREYKALELVLEKRLSKRWQLIGNYRYSSLRGNY
                     EGLFRNDNGQGNPNFTTLFDFPDSPLMRGQFITGPLNLDRPHVLHVLGTRFFDGGFEV
                     GGAVQWMSGLLRTPMLIHPAYLSNNEVPGVDPVYRIWDGVAGQSTFMASNEPQQRGSL
                     GRAPSITTFDLHVAYGVPLGRTSLKVGVDVFNVFNSQHATTFNDTLELGMGVPNPNYG
                     RVNGYEQPRTVRIATIWGW"
     gene            9169..9912
                     /gene="lexA"
                     /locus_tag="VFD06_05670"
     CDS             9169..9912
                     /gene="lexA"
                     /locus_tag="VFD06_05670"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZN03057.1"
                     /translation="MRGNGADRITPLDIRLIYPHAAVIVDGRAEMAASPGTARDRGER
                     MAEITDLTEKQQAVYDFIKRFIGERGMAPSYEEIRKAFRFRSYNSVQKYLKRLEEKGH
                     IKSPWGNRKRALELTDPGPMQRTIPLLGTVAAGRPIEAIQTPDTVEVPETLLRGDDHF
                     ALKVVGDSMVDDGIREGDIIIVKRQRDARNGQTVIALIGDDATVKQYHRRGNQIELRP
                     ANSHMRPIFVQEGDIQIQGIVIGLIRRFQ"
     gene            9909..10148
                     /locus_tag="VFD06_05675"
     CDS             9909..10148
                     /locus_tag="VFD06_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016862294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZN03058.1"
                     /translation="MSDSDRERTSLRVFNAVVERDLDSGLYVGYVPGWPGAHSQGSSL
                     DELHDRLQEVVAMLLEDGALPRASEFVGVQTIRVA"
     gene            10148..10612
                     /locus_tag="VFD06_05680"
     CDS             10148..10612
                     /locus_tag="VFD06_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HZN03059.1"
                     /translation="MTDALIVTLGLFLLAAAEPPSKTLHAKGTFEVQMTPAGEGDKAP
                     GATLGRSTIAKQFHGDLEGTSTATMLTAMTEVKGSAGYVAIERVTGVLNGRRGTFVLQ
                     HSGTMTRGAQQLSVTVVPDSGTGELAGITGKMVITITGGKHEYDFEVVIPRQ"
BASE COUNT         1787 a         3607 c         3787 g         1708 t
ORIGIN      
        1 agttccacag gaggtcggag ggctggtggt tgtcgtcgac gagggtgctg cgggtcgtcg
       61 ggttctgccg ctgtgccgga gtctgcccgt tggggggctc gatgttgatc ccgatgttga
      121 tgttgggggt caccgctccc gcggtcacga cgaccaggct ggcggcgtcg gagttgtccg
      181 acgccgttcc cgggctgacg ccgtcgcctc cccaggggga ctccgccgcg tcgtagaact
      241 cggccaacgc cggcaggaag cccaggacgc cggccgaacc gctgcccggc gccacgttcg
      301 agtttccgac cgtgaggttg tacttctggg agagccggcc cgggagcagc cccgggaaaa
      361 gatcactgct gtcgaagtat tcagcgtaga cgtagtagtt gccgggcggc aggccgtcga
      421 tccggtagaa gccattcccc tgaaacgtcg catccggtcc ttccgggccg agcgccgcaa
      481 tgtcttcgaa acgggagagc gacaggcgcc ccgccacgcc cagcatcgtg accggatcga
      541 tcgccaccac gtggactccg tcggccgcga cgttgttgat ggtgatccgc cccgtgatcg
      601 agccgtaggc ggtcccgtag ctcgcttccg ggtaataccg gccaagggtg ctccggtccg
      661 accgcgacag cgcccgctcg cccacaccgt cgctcggtgg gttgggatcg acgaaaaagt
      721 gcatcgtcgc ctgcggcagc ggatcgtggg ccagacccgc gacgtgtccg acctcatggg
      781 tcgcgaggtt gcgcacgtcg tacaactgcg tggagggatg gaggccgccg acggcgccgt
      841 tcgtggccag ggtctcctcg accgttccga agctgttgaa ccggatgtcg gcgtcgatga
      901 gggtgccggc ggggtacgag ccggccggaa agcagatgcc ggccaggatc gggccctgcc
      961 ccggcggaca tcctcccccc tgcgacacga ccaccgggag agtcagggcg gtggagatca
     1021 cctgtgcgcc gaagcccaga ttggacgcga gaacgccgct gtcgcagaag gtcacgacgt
     1081 tgacgccgtc cgccccctga tccctgacgg aggtttcgcc gccgtagacg aactcgatgc
     1141 gtgagtcggg atcgtcctgc caggcgtcga acgcggtctg gatctcgggc aggagcgtct
     1201 ccggcggcgc gttcgggctg ctgtagcagc cggcgaacgt ggtcgggtcg tgataccgcc
     1261 agacgatcgg catggagttg acgtgccaga cccgattgcc gatgcgcgtc ccgttctggg
     1321 tgatcacctc gacgttcccc agtgccagga ggacggccgt gctggcgagg agcgccgccg
     1381 cgacgctcgc cgatgcgacc agcgtccgcc gcctcaccgc cgcacctcgc cccggggcac
     1441 gggaacgaga cgcatccgcg gggattcggg cggcgcgccg gcgccggagg ccaggatgcg
     1501 gatctgctcc aggaaggtcg cgagcggccg cggcgatcga ccggcctgcg ccgccggctg
     1561 tccggtgccg gggtcttcga cggaaccggc gacggtcacg aaccgctccc cggagagcgg
     1621 attgcggtgg acacggaaca cgccctggct gaaaccgacg atgttctgcc tgccgtcggg
     1681 gcggggcgtc gtcatcagca gcacctcctc cccgatttcg aactccggag tcccctcggt
     1741 gcgcatctcg atcggatcga tgcggccgcc gagcatcgtg accgtgagcc gggcggggcc
     1801 tttccccttg gcctcctcga gcacctcgaa gtcggtgtcg gtgaagatcc ccctccgctg
     1861 ctcgtcccag tagacgcggg tcgcgctggc gcgggcgtgg acgacgcgct tcgccgactg
     1921 caccatcgtg gggaggtcca ggaggcgcag gctggtggcc tccgcccgca gagtgaagga
     1981 gcaggcgacg gccaccgcga ggccgatccg tgccgttccc gatcgcatga aatcgttcat
     2041 cgttgcctcc tctcactcga attcgatgat ggcgcgcacc agctcgagat cgacctcgat
     2101 caccccgact tcgaaggccc ggccatcgtc cagggcgccg cgcgcgacga gggtgaagtg
     2161 gctgacctgg tggacgccga agatgtggtt ctgctcgttg tagaagccgg cgacgccctt
     2221 ctggagggag ggcagggcgc ccagctcgcc gatggtcacg acgcgatccg cggtcgccgc
     2281 cgccagttca gcggcgcgca cgaaccagat cgggaccgcc ccccgtccgt gcagcgatga
     2341 caggatcggt ccgacgtcgt tgaactccgc gaacccctcc accagcagtg gacacaggag
     2401 tgcagcggcg tcgaagtagc gcaggatgtt gaaatcggcc cggatgcact ccggcggacg
     2461 gaagaaggga atcgccgtcc actctccgtc ggtcagcgac tcgagcggag cgctggtgta
     2521 gaagttcggg tcgatgtctt ccgggacccg cacgtgatcg ttggcccggg ccacgaccgg
     2581 gacggcgaga cctgcggcga gcgcgagcgt tgcgacgaaa cggtggatgc gtgtcatggg
     2641 cggcctcctt cgcactgatt cggcattgcc acgcccctgc atgcgtcaaa ccggtgcgat
     2701 tcccatcaaa caattccgcg cccgggggtc tgtcgtagaa tcgggcgcat ccggggggcc
     2761 gccccgcatg cccgatccaa ggcagaagcg cgtcaaggaa ctgttccagg cggcggcgga
     2821 cctccgtccg gcggacaggc acgccttcct ggaggcgcag tgcggcgcgg acgacgcgtt
     2881 gcgaaccgct gtcgagtcgc tcctggcggc cggcgaggac aaggacgtcc tgctcgaccg
     2941 gcggccgttc ctttcagcgg ccggctccgg caccgggtca ccggattccg ccagcccgac
     3001 ggccagtctg gggacggctg tcggcccctc cctggtgatc gatcgctacc ggctcctgga
     3061 gaagctcggc gaaggtggga tgggggaggt ctggctggcg gagcagcagg cgccggtggt
     3121 ccggcgcgtc gcgctcaagc tcatcaaggc cggtatggac acggcgcagg tgatcgcccg
     3181 cttcgaggcg gagcgccagg cgctggcgct gatggatcac ccggcgatcg cccgggtctt
     3241 cgatgccggc gcgacgccgg agggtcgtcc ttacttcgtc atggagcccg tgacggggct
     3301 cccgatcacc cagcacagcg accgggagcg gctcgacatc gccgcgcggc tggagctgtt
     3361 catgcaagtg tgcgaggggg tgcagcacgc ccaccagaag gcgatcctgc accgcgatct
     3421 caagccctcg aacgtcctag tgaccgcgca ggacggccgg gcggccccca agatcatcga
     3481 tttcggagtg gcgaaggcga tggcgcagcc gctcacggcg cacacgctgt acaccggcct
     3541 cgggatcctc gggacccccg ggtacatgag cccggagcag gcggacctcg gccgccagga
     3601 cgtcgacacc cgcaccgacg tgtactcgct cggagtgatg ctctacgagc tgctcgtggg
     3661 cgtcctgccc cacgagccgg agcggctgcg ccgggccggc tgggaagggc tcgggcggct
     3721 gctgcgggac gaggagccgg cgacgccgag caagcgcctg accacgctgc cggccgacac
     3781 gctggatgtc tcggcgcgcc tgcggcggct cgacgttccg gcgctgcgcc ggcacgtctc
     3841 gggcgatctc gactggatca ccatgaaggc gatcgagccg gaccgcgagc gccgatacgg
     3901 cagccccgcg gatctggcgg aggatatccg gcggcacctg cgcgacgagc cggtgctcgc
     3961 cgccgccccg acggcggcgt accgtctggg caagttcgtg cggcgtcacc ggttcggcgt
     4021 ggccgcggcc gccgccatcg ggctcgtcgt ggcggcgggc ctggtcggca cgaccacggg
     4081 gctggtgcgg gcgcgccggg ccgagagcga agcgaggaag caggcggccg cctcggaacg
     4141 cgtcgcggga ttcctgtcga gcgttctcgc atcggtcgat ccggcacgca tggggacgac
     4201 gctcgtggac gacctgcggc ggcgcgtgga gaaggtggag cgtggccgcg gcgggagcga
     4261 gacgcaggtg gccgccaccc tggcggcgtt cgatggagcg acgaagggga tcaatcgcgt
     4321 cgacgcggcc cgggacctcg tggaccagga gatcctgtcg cgcgccgggc attcgatcga
     4381 cgagcagatg cggagcgatc cgctgctggc ggcccgcctg cagtcaacgc tcggatcgac
     4441 ctattacgat ctgggcctct tcgagaaagc ggccgtgtac catctcaagg cgctcgagac
     4501 ccggcgccgg gagctcgggc tggagcatcc cgacaccctc cggtcgatgg atgccctggg
     4561 ccgctccctg cacgtcagcg gtcgcatcga cgaggcggag acgacgctcc gacaggcgat
     4621 cgacattcag agccgcgtcc tcggagccga gcacccggac accctgcagt ccatgaacga
     4681 cctggcctac gcgatgtcgt tcgccgggaa gaacaaggaa tcggaggcgc tctaccggaa
     4741 gacgctggag agccgccgcc gggtcctcgg aaacgagtcc cccgatgccg cgctcacgat
     4801 gcggctcctc gccgacaacc aggtcctgca gggacgcttc gccgaagcgg agcagctgta
     4861 ccgggaagcg ctcgcgatcc agcgccgcgt cctcggcccg gaggttcatg agactctgcg
     4921 atcgatgggg aacatggcgc gcgccttgac cggcctcggc cggcacgatg aagccgagcg
     4981 cgtgtcgctc gaggtgatcg atatccgccg ccggaccagc ggcgaggaca gcatctacac
     5041 cgtgctcagt ctcggctatc tcgggatggg gtatgccaac caggggcgca ggcaggatgc
     5101 cgaacgggtc ctcatggagg ccctggagcg ctcgcggcgg agcgaggtgt ccctggtcac
     5161 ggcgctgatc aactacacgc tggcccccat cctgcgctca cagggacgcc tgccggaggc
     5221 ggagacgctg taccgggaga ccgtggagat ccagagaggg actggctcgg ggagggcagt
     5281 cgccggcacg ctgcggctgc tcgcggaggt tctggtgcaa gaagatcgat gtcaggaagc
     5341 cgagcccgtc taccgtgaga tcgtgaccat ccaggaacgg gagttcggcg cggacgcatc
     5401 tccgacgatt gccgccaaat gggacctcgc gcgactgtac tggggcaccg tcgatgtgga
     5461 gcgcgggcgg tccaacgtcg cgtcgttcct ggccgacctc gagcgccttg cgaagcagcc
     5521 cgaagcgaag gccgacttcc tgaatctcta cgccggatgc ctgctcatct gcgagccagt
     5581 ggatgtgcgc gacccgcagc gggccttccc gttcgcggag aaagccgtcg ccgcctcggg
     5641 gcggaaggac gccaacatcc tcgacaccct cgccctcgcc tatcacctca cgggacgcga
     5701 ctccgaggcc ctgaagatcc agcgagaggc gatcaccatg ctcccctccg ggccgtcgcg
     5761 gctgcgggag gagctggagc ggaacctggc cacctacgag caggccctgc ggcggcgctg
     5821 actccgccac cgcgcgcgcg gccgcggccg caccgccgtg tcaccagccc cagatggtcg
     5881 cgatgcggac ggtgcggggt tgctcatagc cgttgacgcg gccgtagttg ggattgggga
     5941 cacccatccc caactcgagc gtgtcgttga aggtggtcgc gtgctgggag ttgaagacgt
     6001 tgaagacgtc gacccccacc ttcaacgacg tccgtccgag cggcacgccg taggcgacgt
     6061 gcaggtcgaa ggtcgtgatg gaaggtgccc gccccaggct gccgcgctgc tgcggctcgt
     6121 tgctggccat gaacgtgctc tgcccggcga cgccgtccca gatgcggtaa acgggatcga
     6181 ctccgggaac ctcgttgttg gacaggtagg ccgggtgtat cagcatcggc gtgcgcagga
     6241 ggccggacat ccactgcacc gctccgccga cctcgaagcc tccgtcgaag aagcgggtgc
     6301 cgagcacgtg caggacgtgc ggccggtcca ggttcaacgg accggtgatg aactgtcccc
     6361 gcatcagggg cgagtcgggg aaatcgaaca gcgtggtgaa attcgggttc ccctggccgt
     6421 tgtcgttccg gaagaggccc tcgtagttcc cgcgcaggct cgaataccgg tagttgccga
     6481 tcagctgcca tcgcttcgac aggcgcttct cgaggacaag ttcgagagct ttgtactcgc
     6541 gctccggatc ggcgaatggg tacgcgacat ccttcggagt gttgcggccc gggttcgtca
     6601 ccacgtactg gccgagaggg gccgatccga atcccggaaa tggcaggttg ccgcccgcat
     6661 ccgggcccag ggagaagttg aggaactcct cggccgaggt ggactgcacg tcctcgagca
     6721 cgcgtccctg atcgcggagg atggcgcgga tttcgaacat caggtcggcc gacaggagtt
     6781 gctgccagcc gaccacgaac tcgtccacgt aggggagccg cgtgttcttc tccactcgcg
     6841 tcgggaggag ggccgtcatg agaatcgaac tctccggatt ccggaccgtt aggtcgggat
     6901 cgcggaaggg gaaattgagc acgatgatat cgttggagaa ggcgtgcgcc gccagatcga
     6961 gcgggacccg ctcgaagtag cgggcagcgt tcacgtacag cttcgtacgt ccgtccgccc
     7021 gcggatcgaa ggtgagaccg agtctcgggg agatctccgt ctcgaacgtg tacgacacgg
     7081 gctcgaagcg cgtcgggtcg gtggtgaaaa catccggcgc gacgaacgac agattgagcg
     7141 tgaagtcgcc acttccctcg aggctctgcc gcgtggcccg ggctccgagt ttcgccaccc
     7201 agcggcggcc gatcgaccac gtgtcctgca cgaagaggga gtgttcctgc gagttcgtcg
     7261 gcccggacgg gttgagaaac gtgaaactct cgtacgaagg aatctggctg ctacacgcgg
     7321 tgcatccggg gatgccgccg atcaccgtga ccatcgtgcc ggtcggcgat cggatgacgg
     7381 tggtggagtc gaccacgccg tccgcgttcg tgtcgattgg aaaggggaga tcgacgtgct
     7441 taccaccgag gatctgctcg tactcggcat cccaatactc gtacccggcc ttgacctcgt
     7501 gccttccgag gatgccggtg aacttgagcg ccgcgtccca cgcctcctcg tcgctgctcg
     7561 ccggcagccc cccgcctccg cgcgggatga agtcgatccc ggcgccccgg atgatgcgct
     7621 cgtcggtgta gggatagtcg tccacgacgg aggtcgcctc gaatcggctg tggcggtggc
     7681 tgatctggct ctcgaaaaac cagttcggac cgaaaaccgc gctgtagcga agagcctggt
     7741 ggccggatcc gtactcggcg tccgtcctca acgccccatc gagatagccg gcggcggtct
     7801 tggtgacgtc gagggtgccg tccccatcga ggtcgccggc caggttgaag ggatttccgg
     7861 cgccgatccc gagactgccg tcaccgcggg attcgtcccc gaatccggac agctcgagcc
     7921 ggtgccgcgg cgagacctgc caggtcacct tggcggcgta attgaaacgc ctctggtcgt
     7981 cgcgccgccc gtccagggct tccggcggtt gcagctccgc gtacgggtag gtctcggcgg
     8041 cgccgggatc gaacgccagg acaggattcg tggtcgtcgg cgcatcccgg aacaccagcg
     8101 agctgtcgtg cacggggttg agtgcgacga accagaagag tttgtccttg atgaacggcc
     8161 ccccggcctg cacgctgacg tcggttctct cgcgatcttc gacctgggtg gcgcctccct
     8221 cgatcacgat ctcttccccg ggagcctcca gatcggccgg ttggaagaag aagcggatcg
     8281 agccggtgaa tgcgttcccg ccggacttga cgacgccatt gatcacgccg cccagcgcct
     8341 ggccgtactc cgcctcgaag ccggcggtct tgacctgaag ctcctccagg aagtcgaggc
     8401 caatccccgt ggagatggtg cggccgaaca gcccggtgcc gacgctcccg aaaccgacac
     8461 tggtgatgtt ggtgccatcg atgatgtagg cgttctccag ccccgaggag ccgccgatgg
     8521 agtggttgag ctccccggtg ccgtggccgg ggacgacgcc cggtgcgagc cgggcgagat
     8581 cggtgaattc tcgcccgacc gggagcgtgg agatcacctc gtcggtcttg aaggtcgtga
     8641 cgacctcggt gcgcttcatg tccacgagcg gcgcggcgcc ggtcacctcg atctcctcgg
     8701 tgtatccggt ggtcagcacg atcggcagcc aggtccgctc ccccgcggcg accgtgacct
     8761 cctcgatcac gatcgtgccg aagccgggct tctccacctg cacccggtag tcgccggcgg
     8821 ggaggaggcg gatctcgacg cggccgttgt cgtcggcggt gacgacacgc tggcccagcg
     8881 gtcccgcgac ggtgagcttc gcctccgcca ccggcgcgcc ggtctgatcg gtgacgctgg
     8941 cgacgaagct cccggtggtc ggctgctggg cggacaccac ctcagccgcg gcgaggccag
     9001 cggcgagcac gatgagccgg cacgttgttt tcgtgaccga actccccatt gggccgtgcc
     9061 cgagcgacga gcaggtatct ttgccgcata gactaaccga ggtgcgcctg atttcgaccc
     9121 ccccgggttc ggcggcgcct gccccctggg cttgccggcc ctcccggcat gcgcggaaac
     9181 ggcgcggatc gcatcacccc tcttgacatc cgcttaattt acccccatgc tgcggtcatc
     9241 gtggacggcc gggcggagat ggcggcttcc cccgggacgg cccgcgaccg aggggagcgg
     9301 atggccgaga tcaccgacct caccgagaag cagcaggcgg tctacgactt catcaagcgg
     9361 ttcatcggcg agcgcggcat ggccccctcg tacgaggaga tccgcaaggc cttccgcttc
     9421 cggtcgtaca actcggtcca gaagtacctg aagcggctcg aggagaaggg gcacatcaag
     9481 agcccctggg ggaaccgcaa gcgggccctc gagctgaccg accccgggcc gatgcagcgg
     9541 accatcccgc tcctcggcac cgtcgccgcc ggccggccga tcgaggcgat ccagaccccc
     9601 gacacggtgg aggtccccga gaccctcctg cgcggcgacg accacttcgc cctcaaggtg
     9661 gtcggcgaca gcatggtgga cgacggcatc cgcgaagggg acatcatcat cgtcaagcgc
     9721 cagcgcgacg cgcgcaacgg ccagactgtc atcgccctca tcggcgacga cgcgaccgtg
     9781 aagcagtacc accgccgcgg caaccagatc gagctgcgcc ccgccaactc ccacatgcga
     9841 ccgatcttcg tgcaggaggg ggacattcaa attcagggaa tcgtgatcgg cctcatccgg
     9901 aggttccaat gagcgacagc gaccgggaac ggaccagcct gcgcgtcttc aacgccgtcg
     9961 tcgagcgcga cctcgacagc ggcctctacg tcggctacgt cccgggctgg cccggggccc
    10021 acagccaggg ctccagcctc gacgagctgc atgaccgtct ccaggaggtc gtcgcgatgc
    10081 tgctcgagga cggcgcgctg ccgcgggcgt cggagttcgt cggagtccag acgatccggg
    10141 tggcgtgatg accgacgcgc tgatcgtgac tctcgggctg ttcctgctgg cggccgccga
    10201 gcctccttcc aagacgcttc acgcgaaagg cacgttcgaa gtccagatga ccccggccgg
    10261 ggagggggac aaagccccgg gcgcgacgct cggccggtcc accatcgcca agcagttcca
    10321 cggcgacctc gaggggacga gcaccgccac catgctcacc gccatgaccg aggtcaaagg
    10381 ctccgccggc tacgtggcga tcgagcgcgt gaccggcgtg ttgaacggcc gccgcggcac
    10441 cttcgtcctc cagcacagcg gcacgatgac ccgcggcgcg cagcaactct cggtgacggt
    10501 ggtgcctgat tccgggacgg gggagctggc ggggatcacc ggcaagatgg tgatcacgat
    10561 caccgggggg aagcatgaat acgacttcga ggtagtgatt ccacggcagt gatgagcagc
    10621 tcgaactcag aggtactgtg tggcacgtga cgaattttca aaatctgtca agttagcgct
    10681 catgattcgg gctgggttga gatgttcccg tccagactgt gccgccacca cctgtgggcc
    10741 acattcgaac ccgagtaaat ggctaagttt cggcgaggct tgtcatgtta ccgcggcttc
    10801 gccaggcggt cccagatatg attcgtccct tacacctgaa caacgagcag atatctcgaa
    10861 cggtctatgg ctgtgcgcat cctgtgcca
//
LOCUS       DASRIQ010000230         4670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_97106, whole genome shotgun sequence.
ACCESSION   DASRIQ010000230 DASRIQ010000000
VERSION     DASRIQ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97106"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFD06_05685"
     CDS             <1..437
                     /locus_tag="VFD06_05685"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN03060.1"
                     /translation="ETPARSGGGHDPFLSIVAEDPHGRSLRFADFKGKVRVIDLWASW
                     CGPCRMTIPELNALYERYRERGLVVIGVSVDEQPAAVLEFQRQVPVRYPSVMFNPPLA
                     FVIGQPSSIPTTYVVDRDGEVRKTFVGYVDAATLEEAVRQLL"
     gene            521..1147
                     /locus_tag="VFD06_05690"
     CDS             521..1147
                     /locus_tag="VFD06_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03061.1"
                     /translation="MKRRVRLGDVIDDYCTRCRRIMNHGIVGMVGDDVAKVRCNTCQS
                     EHAYKHGRLPKRRRGETDRLFAEVLRGIRGAPDPSEEADEERARPEPGEGADEEPGDV
                     PAPRAAAGDADREDEPDAADEPDAAPGESDLPARPETDDHPAHGVRRRLFTIRRHSGG
                     KAPVEGVSTDIGRPGGPVTGNRPGGPAGGRGGRPQHGGRWPRRHGRRR"
     gene            complement(1173..2615)
                     /locus_tag="VFD06_05695"
     CDS             complement(1173..2615)
                     /locus_tag="VFD06_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZN03062.1"
                     /translation="MSPKVKSERSVTSAGPGSERAVAGERVYDDIVAAIGHTPTVRLN
                     RLGRDTGATLYAKLEYMNPGSSIKDRIAVQMIADAEQAGTLKPGGTIIECTSGNTGMG
                     LAMAGTVRGYRTILVMPDKVSDEKIKALRAFGARVITTPTAVAPDDPRSYYSVAQRLN
                     REIPNSFLANQYHNPSNPESHYRTTGPEVWDAFGDKLDAVVIATGTGGTITGIGRYLK
                     ERKPSIRMVLIDPVGSILYDYFKTREVMKSFKTYKVEGFGEDFIPSSLNIDIVDDCYQ
                     VTDKECFLTARELTRKEGLFTGGSGGGAVCGAIKFAREHPECKTILVILPDSGSRYLS
                     KVYDDDWLRENSFLDDEETFGRLSDLMERQEQTLITARPGDEVKGIIRLMKQHGISQL
                     PVLEGDRLVGIISEGDLVQALLQDPEIINRPIEELVDQSYVVVPPDTPLTRLAAIFGE
                     GKVALVVEQGKVRSLVTKIDLIDHMAGQLR"
     gene            complement(2625..3365)
                     /locus_tag="VFD06_05700"
     CDS             complement(2625..3365)
                     /locus_tag="VFD06_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZN03063.1"
                     /translation="MSGGGARDGTLIAAVSVLAFRDGRLLSLRRSPRKDAAPGAWEVI
                     SGRIEPGEQPTEAAAREGREESGFDLAIDPRPIAAYRGDRLGRDMLVVAFRADAPPGD
                     PVLSDEHDDWAFVTLEEFARRCPFPRLVAVADLAAEAAGPSGAAAPAPAHVLVWEFRP
                     APGREAEFERAYGPDGDWARLFRRDPAYLGTELLRGTAGRYLTLDRWRSRAAYEGFRA
                     RHADDYADLDRRMEDLTSHESSIGAFDV"
     gene            3409..4458
                     /gene="hflK"
                     /locus_tag="VFD06_05705"
     CDS             3409..4458
                     /gene="hflK"
                     /locus_tag="VFD06_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HZN03064.1"
                     /translation="MAQDDGDFPAGGGGPRRVRNQPFGNVDLGSLTTGRLLGAAWLVA
                     AALLGLIGVLSSWYQVESDEVAIVQRFGAYVRTTEPGLHMKIPFLVERVTKVPVRRQL
                     KMEFGFRTTHVGVQSQFAPPTAETRAESLMLTGDLNVAEVEWSVQYRIKEPRQYLFHV
                     RDVDRTFRDMSQAAMRHVVGDHSVDEVITIGREGIAAEAKEELQRLCDLYGIGIDVQQ
                     LVLQDVNPPDPVKPAFNEVNQAIQEKERAINDAWAEYNRTVPLAKGQADQAVRSAEGY
                     ALERVNNAEGDARRFSAIQAEYSRAPLVTRRRLYLETMTEVLPKAGRRFIVDDKAKGI
                     LPLLNLSVPAKEATP"
     gene            4481..>4670
                     /locus_tag="VFD06_05710"
     CDS             4481..>4670
                     /locus_tag="VFD06_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN03065.1"
                     /translation="MKSALLFGWIALIAIVTFASTFTISETEQVVITQFGRPVGGVIA
                     SPGLHLKTPFVQEVHRFDK"
BASE COUNT          697 a         1624 c         1646 g          703 t
ORIGIN      
        1 gcgagactcc cgcccggtcc ggaggaggac acgacccgtt tctgtccatc gtcgccgagg
       61 acccgcacgg ccggtcgctg cgcttcgccg acttcaaggg gaaggtgcgg gtgatcgatc
      121 tctgggcgtc gtggtgcggc ccctgccgca tgacgatccc ggagctgaac gcgctgtacg
      181 agcgctatcg cgagcgcggg ctggtggtga tcggggtgtc ggtggacgag cagccggcgg
      241 cggtcctcga gttccagcgc caggtcccgg tgcgctaccc gtccgtgatg ttcaacccgc
      301 cgctggcctt cgtgatcggc cagccgtcgt cgatcccgac cacgtatgtg gtcgatcgcg
      361 acggtgaagt ccgcaagacc ttcgtcggct acgttgacgc cgcgacgctc gaagaggcgg
      421 tccggcagct gctttgatgc tcggacagac tcccgggctt cccaatcggg ccccgagagg
      481 ctaccatgac cgcctccagc cccccgcctt cggaggcccg atgaaacgac gcgtccgcct
      541 gggggacgtg atcgacgact attgcacccg gtgccgtcgg atcatgaacc acggcatcgt
      601 cggcatggtc ggcgacgacg tcgccaaggt ccgctgcaac acctgccagt ccgagcacgc
      661 ctacaagcac gggcggctgc cgaagcgccg ccgcggcgag accgatcgtc tgttcgcgga
      721 ggtcctgcgc ggcatccgcg gagcgccgga tccttccgaa gaggcggacg aggagcgggc
      781 gcgcccggag ccgggggagg gggcggacga ggagccgggg gacgtgccgg ccccgagggc
      841 cgcggcgggg gacgccgatc gagaggatga gcccgatgcg gccgacgagc cggacgccgc
      901 gccgggggaa tcggatctgc cggcgcgtcc ggagaccgac gaccatccgg cccatggcgt
      961 tcgacggagg ctcttcacca tccgccgcca cagcggcggc aaggcgccgg tggagggggt
     1021 gtccaccgac atcggcaggc cgggagggcc ggtgacggga aaccgccccg gcggtccggc
     1081 cggcgggcgc ggcgggcggc ctcaacacgg cgggcgctgg ccacggcgcc acggacggcg
     1141 ccgctgaatc gacgccgcca cggacggcgc cgctaacgca gctgccccgc catgtggtcg
     1201 atgagatcga tcttcgtgac caggctgcgc accttcccct gctcgacgac gagcgccacc
     1261 ttcccctcgc cgaagatggc ggcgaggcgg gtcagcggcg tgtcgggggg aacgaccacg
     1321 tacgactgat cgacgagctc ctcgatcggc ctgttgatga tctccgggtc ctgcagaagc
     1381 gcctgcacca ggtccccctc ggagatgatg ccgacgagac ggtccccctc gagcaccggc
     1441 agctgcgaga tgccgtgctg cttcatgagg cggatgatcc ccttcacctc gtccccgggc
     1501 cgggcggtga tgagcgtctg ctcctgccgc tccatgaggt cgctcagcct cccaaatgtt
     1561 tcttcgtcgt cgaggaagga gttctccctg agccagtcgt cgtcgtacac cttgctgaga
     1621 tagcgcgagc cggagtcggg gaggatcacc aggatcgtct tgcactccgg gtgctctctg
     1681 gcgaacttga tggcgccgca caccgccccg ccgccggagc ctcccgtgaa cagcccctcc
     1741 ttgcgggtca gctcgcgcgc cgtcaggaag cactccttgt cggtgacctg gtagcagtcg
     1801 tccacgatgt cgatgttgag gctcgacggg atgaagtcct cgccgaaccc ctcgaccttg
     1861 taggtcttga acgacttcat cacctcgcgc gtcttgaagt agtcgtacag gatcgagccg
     1921 accgggtcga tcaacaccat gcggatggac ggcttgcgct ccttgaggta gcggccgatg
     1981 ccggtgatcg taccgccggt gccggtggcg atcaccaccg cgtcgagctt gtcgccgaac
     2041 gcgtcccaga cctccgggcc ggtggtgcgg tagtgcgact ccgggttgct cgggttgtga
     2101 tactggttcg cgaggaagga gttcgggatc tcgcggttga ggcgttgggc gaccgagtag
     2161 taggagcgcg gatcgtcggg ggcgaccgcg gtcggcgtcg tgatgacgcg ggcgccgaag
     2221 gcgcgcaggg ctttgatctt ctcgtccgag accttgtccg gcatgacgag aatcgtgcgg
     2281 tagccgcgca ccgttcccgc catcgcgagg cccatgccgg tgttgccgct ggtgcactcg
     2341 atgatcgtcc cacccggctt gagcgtcccc gcctgctcgg cgtcggcgat catctggacc
     2401 gcgatccggt ccttgatcga cgacccgggg ttcatgtact cgagcttggc atagagggtc
     2461 gcgccggtgt cgcgtccgag gcggttcagc cggacggtcg gggtgtggcc gatcgccgcg
     2521 acgatgtcgt cgtagacccg ttcgcccgcc accgcccgct cgctcccggg gccggcgctc
     2581 gtcaccgatc gttcgctctt caccttgggg ctcatcgacg cattctacac atcgaaggcg
     2641 ccgatcgagg actcgtgcga ggtcagatct tccatccgcc ggtcgaggtc ggcgtaatca
     2701 tccgcgtgcc gcgcccggaa cccctcgtac gcggcgcgcg agcgccagcg gtccagggtg
     2761 aggtagcgcc cggccgtgcc gcgcagcagc tcggtcccga gataggccgg gtcgcgccgg
     2821 aacaggcgcg cccagtcgcc gtcgggaccg taggcccgct cgaactccgc ctcgcgcccg
     2881 ggggcggggc ggaactccca caccaggacg tgcgccggcg ccggtgcggc cgcgccgctc
     2941 gggccagccg cttccgccgc gaggtcggcg accgcgacga gccgcggaaa cgggcagcgg
     3001 cgggcgaact cctcgagcgt cacgaaggcc cagtcgtcgt gctcgtccga gaggaccgga
     3061 tcgcccggcg gggcgtcggc ccggaaggcc accaccagca tgtcccggcc caggcgatcg
     3121 ccgcgatagg ccgcgatcgg ccgcgggtcg atggcgagat cgaacccgct ctcctcgcgc
     3181 ccctcgcgcg ccgccgcctc ggtcggctgc tcgccgggtt cgatgcggcc cgagatcacc
     3241 tcccacgcgc ccggggcggc gtccttgcgc ggcgagcggc gcagcgacag caggcgcccg
     3301 tcccggaacg ccagcaccga caccgccgcg atcagcgtcc cgtcgcgcgc tccgccgccg
     3361 ctcatcctcc tcgcctctcg gagacctgtg gtatagatcg cggccatcat ggcacaagat
     3421 gacggcgact tccccgcggg gggaggaggt ccccgtcgcg tccgcaacca acccttcggc
     3481 aacgtcgatc tcggctccct gaccacgggg cgtctgctgg gggccgcctg gctcgtggcc
     3541 gccgccctcc tcgggctcat cggcgtcctg tcctcctggt accaggtcga gtcggacgag
     3601 gtcgccatcg tgcagcgctt cggcgcctac gtccgcacga ccgagccggg gctgcacatg
     3661 aagatcccgt tcctggtcga gcgggtcacg aaggtgccgg tccgccgcca gctcaagatg
     3721 gagttcggct tccgcacgac gcacgtcggc gtgcagtcgc agttcgcgcc gccgaccgcc
     3781 gagacgcgcg ccgagtcgct gatgctcacc ggcgacctca acgtggcgga ggttgagtgg
     3841 agcgtgcagt accgcatcaa ggagccgcgc cagtacctgt tccacgtgcg cgacgtggac
     3901 cgcacctttc gcgacatgtc gcaggcggcg atgcgccacg tggtcgggga ccacagcgtc
     3961 gacgaggtga tcaccatcgg ccgcgagggg atcgccgccg aggcgaagga ggagctgcag
     4021 cgcctgtgcg acctgtacgg catcggcatc gacgtccagc agctcgtcct gcaggacgtc
     4081 aacccgcccg atccggtgaa gccggcgttc aacgaagtga accaggcgat ccaggagaag
     4141 gagcgggcga tcaacgacgc ctgggccgag tacaaccgca cggtgccgct ggccaagggc
     4201 caggccgacc aggcggtgcg ctcggcggag gggtacgccc tcgagcgggt gaacaacgcc
     4261 gagggggatg cccgccgctt ctccgccatc caggcggagt acagccgcgc gccgctcgtg
     4321 acgcgccggc ggctctatct cgagacgatg accgaggtcc tgccgaaggc cggccgccgg
     4381 ttcatcgtgg acgacaaggc gaaggggatc ctgcccctcc tgaacctgag cgtcccggcg
     4441 aaggaggcca cgccatgacc cggcagcggc gggtcaagag atgaagtcgg cgctgttgtt
     4501 cggatggatc gccctgatcg cgatcgtgac gttcgcctcg accttcacga tcagcgagac
     4561 cgagcaggtg gtgatcaccc agttcggccg gccggtcggg ggcgtcatcg cctccccggg
     4621 gctgcacctc aagacgccgt tcgtccagga ggtgcaccgc ttcgacaagc
//
LOCUS       DASRIQ010000231         7365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3762857, whole genome shotgun sequence.
ACCESSION   DASRIQ010000231 DASRIQ010000000
VERSION     DASRIQ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3762857"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /gene="tyrS"
                     /locus_tag="VFD06_05715"
     CDS             <1..771
                     /gene="tyrS"
                     /locus_tag="VFD06_05715"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZN03066.1"
                     /translation="LERDDFMKRYKAQQPIGLHEFMYPLAQGYDSVALEADVEMGGTD
                     QTFNLLVGRDLMREYGLEPQVVLTLPLLEGTDGVEKMSKSLGNAIGIDESPREIFGKT
                     MSLSDDLMWRYYTLCTDLTLRDIEALKGRVAAGETHPKQAKADLARRIVADFHGETAA
                     AEAAAEFERIFARKENPDEIREVRMPAVAEPLWIPKLLVTAGLAKSNGEARRLIEQGG
                     VSLDGERLSDPAATLAARPAASHLFKVGKRHFVRIVFD"
     gene            complement(789..1430)
                     /locus_tag="VFD06_05720"
     CDS             complement(789..1430)
                     /locus_tag="VFD06_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03067.1"
                     /translation="MNARFVPRVLLLAAALAVVAVPSAHGVARDVVNEARSAYEGRTL
                     RLRLDLRSSASAIEPNTLTLEGIGYGRPDAPVLFGRLETVHLDRVASEGGDRLSLTVY
                     RSREEMQQLRATAIPPPTIGGTPIGMQPMSGFARIGSTMVIFDLLAKKKDIAGQRAEI
                     DTLMRRLFYIDDAPERGELEEFVRQHHSWPVPRLASVTGLSQDEVRAILEAKP"
     gene            1460..2845
                     /locus_tag="VFD06_05725"
     CDS             1460..2845
                     /locus_tag="VFD06_05725"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HZN03068.1"
                     /translation="MPPPEAHHFVPPLAAVLPFAGLLLSIAILPARVPRFWHRHYHRV
                     ALAWAGAYAIPALLWRPGAAVHDLLHVVLTEYLPFLVLLGALFIISGGIVLRGRLRGT
                     PAHNTGLLAFGTILASFIGTTGASMVLIRPLLRANAWRIRRGHVVVFFIFLVANIGGA
                     LTPLGDPPLFLGFLQGVPFFWTLKLLPHFLLASGLLLAAFFALDTWALRHEPAARPGG
                     PLSAPAAGREPAVGRERIGIDGAVNLGLLAAVLGAVLLSGVWHPGSVSIFGVEWKVES
                     ALRDVVLLLLAGLSLRLTPDRLRQDNGFSWEPIREVAWLFAGIFVTIIPAIALLRAGE
                     GPVATLLRELSEPWQLYWATGLLSSVLDNAPTYLSFFNALLGRLAPGMSSAEGVARLT
                     TEGALWLEAISAGAVFMGAMTYIGNAPNLMVRAIAEDAGVSMPTFGGYLLRYALPILL
                     PVLVLVQVALF"
     gene            complement(2850..3887)
                     /locus_tag="VFD06_05730"
     CDS             complement(2850..3887)
                     /locus_tag="VFD06_05730"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HZN03069.1"
                     /translation="MSRWFGALLLPALLVCSIGAAAPPSGSAPPAAAADLFRRFAGAW
                     DATMQVVGPPGEPPQILNGIEVNTTGGGGLWVTGDFRSQIDGRPFQGHSIVTWDAPSS
                     KFRRLWADSTGTTFWISEGTWDPKKATLTMWIETRNSSGEPVRWREETIFEEAGRTFT
                     MYIPGPTTVEAAAMTITYRRRSAAQKVPPVTGPAPAPAGPELARLQKSMGVWSVRRED
                     RAGGEAMKGTETNAWCCDGRFLVTDVEGRSGKRPYAGHGILAYDPEQKRYERAFVDTA
                     GAALERVPGAWDESAGTLTFHVDRPDGRGGTLHAEEVLAWDGDERTTTLSQVSSGGGK
                     VLATSRYRKER"
     gene            3976..>7365
                     /locus_tag="VFD06_05735"
     CDS             3976..>7365
                     /locus_tag="VFD06_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HZN03070.1"
                     /translation="MLRRPPSIRKRRTVPSLWAGIAAALLFASFAAPARAANHLAGAT
                     SPYLLEHADNPIDWYPWGPQALEKAKREDKPIFLSVGYFSCHWCHVMERESFTDQEIA
                     GLLNEGFVAIKVDREERPDLDALYMNAVIAMTRGGGWPMSVFLTPDLRPFHAGTYYPK
                     ARFRDLVGSIGIAWRSERARLLEAAGTLRDAMAEIQKTPDEAGEDDPSTDLLALATKA
                     LAKDFDAEHGGFGRAPKFPPHGALTLLLRADRQGDAQALRMAAATLEAMRRGGIRDQI
                     GGGFHRYAVDAAWKVPHFEKMLSDNALLAPIYFMAWKQTGYGEFRRVAEETLAFVERE
                     MSDPAGGYVTALDADSDGEEGRYYTFTVDEIRAAVPGKDADLVLEYYGVTPKGSVARG
                     RSVLAPTEKDEAFASRHGLSIQDWFTRLEAARKKLLQARSRRPAPHRDDKVLTAWNGL
                     MITALASAHVATGDAAHLTRARRAADFALKELVGPDGRARVSWRRGRPGGPGFLDDTA
                     FLIRGLLDLNATDPDRRWMQGATALAKDAARFADPSGGWFFAADTPDLIVRPRSLDDS
                     ALPAGNAIMVENLARLAHLSGDLGALQEAKRTLDRGAAVMRRAPTAHPYLILARETLK
                     QSRAHGPAADAASAPGLLSRPALAADTGAARAAASPASAPASDAVSAGQAAAGAGATR
                     DVPGQVVGRANRERVVESVLKAPSQPVRPGAAAPLEFVMTIRDGWHTNSSRPTLEYLI
                     PTKVTFTDTGRGAAEGIEYPDGKMVKLQFADEQLSVYEGKIGIRARLRVPPDAPAGPM
                     AFVARLNYQSCSDKACLPPETVEFKGAVEVGGAPLSEAERAAEAPAPPSEGGAGAAAG
                     GPATGATPGGAGSVASAPLKGRDGQDQLSLLLRERGMLFVIGFVFLGGLALCLTPCVY
                     PMIPVTLGYFSQQAEGASWGRRIGLPSLYVLGIAITYSVLGVVVGLTGGLFGSMMQNP
                     IVVGVLILLFVAMAFWMFGVYELRLPGFLTRFGGGRAGAVGAFVMGLTLGLVAAPCVG
                     PFIVTLLAFVAATGSPVIGFWLFFVLALGMGLPFLVLGIFSGALSSLPRSGAWLLYAK
                     KVMGVALLGVAIYFMQPFLSDRVLGWLAIAFALGAGVWLALI"
BASE COUNT          972 a         2619 c         2606 g         1168 t
ORIGIN      
        1 ctcgagcggg acgacttcat gaagcgctac aaggcccagc agccgatcgg cctgcacgaa
       61 ttcatgtacc cgctggctca ggggtacgac tcggtggcgc tcgaggcgga cgtcgagatg
      121 gggggaaccg accagacctt caacctgctg gtcgggcgcg atctcatgcg ggagtacggc
      181 ctcgagcccc aggtggtgct gacgctgccg ctcctcgaag ggaccgacgg ggtcgagaag
      241 atgtcgaagt cgctcggcaa cgccatcggc atcgacgagt ccccccgcga gatcttcggc
      301 aagacgatgt cgctctcgga cgacctgatg tggcgctact acacgctgtg caccgacctg
      361 acgctgcgcg acatcgaggc gctgaagggt cgcgtggcgg cgggggagac gcacccgaag
      421 caggcgaagg cggacctcgc ccgccgcatc gtcgccgact tccacggcga gacggcggcc
      481 gccgaggcgg cggcagagtt cgagcggatc ttcgcgcgca aggagaaccc ggacgagatc
      541 cgggaggtcc ggatgccggc cgtggcggag cctctgtgga ttccgaagct tctggtgacg
      601 gcggggctgg cgaaatccaa cggcgaggcg cgccgcctca ttgagcaggg aggggtgagc
      661 ctcgacggcg agcgcctctc cgacccggcg gcgaccctcg ctgcgcgccc cgccgcctcg
      721 cacctgttca aggtcggcaa gcgccacttc gtgcgcatcg tcttcgactg accgcgtgcg
      781 cttgtgaatc agggcttggc ttcgaggatc gcccgcactt cgtcctgcga cagcccggtg
      841 accgatgcga ggcgcggcac cggccacgag tggtgctggc gcacgaactc ctcgagctcg
      901 ccgcgctccg gcgcatcgtc gatgtagaaa agccgccgca tcagagtgtc gatctcggcg
      961 cgctgccccg cgatgtcctt ctttttcgcg agcaggtcga agatcaccat ggtggagccg
     1021 atgcgcgcga acccggacat cggctgcatc ccgatcggtg tcccgccgat ggtcggcggt
     1081 gggatggcgg tggcgcgcag ctgctgcatc tcctcgcggc tgcggtaaac cgtcagcgac
     1141 aggcggtccc ccccttcgct cgccacccga tcgaggtgaa ccgtctcgag ccggccgaac
     1201 agcaccggcg cgtcggggcg cccgtagccg atcccctcga gcgtcagggt gttcggctcg
     1261 atggcggagg cggaggatcg caggtcgagc cgcagccgga gcgtgcgccc ctcgtaggcg
     1321 ctacgcgctt cgttgaccac atccctcgcc accccgtgcg cggacggaac ggcgacgacc
     1381 gcgagcgccg cggcgaggag caggacccgg gggacgaacc tcgcgttcac gtctctcgat
     1441 tgtaatagga tgccccgccg tgccgccgcc cgaggcccat cacttcgttc cgccgcttgc
     1501 agcggtgctg ccgttcgccg ggctcctcct ctccatcgcc atcctgccgg cccgggtccc
     1561 gcgcttctgg caccggcact accatcgggt cgccctcgcc tgggccgggg cctacgccat
     1621 tccggccctg ctgtggcgcc cgggggccgc ggtccacgat ctcctgcacg tcgtcctgac
     1681 ggagtacctg ccgttcctcg tccttctcgg agcgctcttc atcatctccg gcgggatcgt
     1741 cttgcgcggc cggcttcgtg gcacgccggc gcacaacacc gggctcctcg ccttcgggac
     1801 catcctcgcc tcgttcatcg ggacgaccgg cgcgtcgatg gtgctgatcc ggccgcttct
     1861 ccgggccaac gcgtggcgca tccgccgcgg tcacgtggtc gtcttcttca tcttcctcgt
     1921 cgccaacatc gggggagcgc tgacgccttt gggcgatccg ccgcttttcc ttggattcct
     1981 gcagggcgtg ccgttcttct ggaccttgaa gctcctgccg catttcctcc tcgcctccgg
     2041 cctcctgctc gccgccttct tcgctttgga cacgtgggcc ctgcggcacg agccggcggc
     2101 gcgtcccgga ggaccgcttt ccgcgccggc ggccgggagg gagcctgcgg tcgggaggga
     2161 gcgaatcggg atcgacggcg ccgtcaacct gggcctcctg gcggcggttc tcggggcagt
     2221 gctcctctcc ggcgtctggc acccgggatc ggtttcgatc ttcggcgtgg aatggaaggt
     2281 ggagagtgcc ctgcgcgacg tggtcctgct cctcctggcg gggctgtcgc tgcgtctcac
     2341 gccggatcgg ctgcggcagg acaacggctt ttcctgggag ccgatccgcg aggtggcgtg
     2401 gctcttcgcc gggatcttcg tcaccatcat cccggccatc gcgctcctgc gcgccggcga
     2461 ggggccggtg gccacgctcc tcagggaact gtcggagccc tggcagctgt actgggcgac
     2521 cggcctgctc tcctcggtgc tcgacaacgc gccgacctat ctgagcttct tcaacgccct
     2581 tctcgggagg ctcgctccgg ggatgtcgtc cgcagaaggg gtcgcgcgcc tgacgaccga
     2641 gggggcgctg tggctcgagg cgatctcggc cggggcggtg ttcatggggg cgatgaccta
     2701 catcggcaac gcccccaacc tgatggtgcg ggcgatcgcc gaggatgcgg gcgtgtcgat
     2761 gccgacgttc ggcggttatc tgctgcgcta cgccctgccg atcctgctgc cggtgctcgt
     2821 cctcgtgcag gtggcgctgt tctaggagcc tagcgctcct tgcgatagcg gctcgtcgcc
     2881 aggaccttcc cgcctcctga ggagacctgc gacagcgtgg tggtgcgttc gtcgccatcc
     2941 caggcgagca cctcctcggc gtgcagcgtc ccgccccggc catcggggcg atcgacgtgg
     3001 aaggtgagcg ttccggccga ctcgtcccag gcgcccggga cccgctcgag cgcggcgccg
     3061 gccgtgtcca cgaaggcacg ctcgtagcgc ttctgttccg ggtcgtacgc cagaatcccg
     3121 tggccggcgt acggacgctt gccggatcgg ccctcgacgt ccgtgaccag gaaccggccg
     3181 tcgcagcacc aggcgttcgt ctcggtccct ttcatcgcct ctccccccgc gcggtcctcg
     3241 cggcgcaccg accagacacc catgctcttc tgcaggcggg cgagctccgg gccggccggg
     3301 gccggcgcag gtccggtgac gggaggcacc ttctgcgccg ccgaccgacg ccggtaggtg
     3361 atggtcatgg ccgccgcctc caccgtcgtc ggtcccggga tgtacatggt gaaggtgcgt
     3421 cccgcctcct cgaagatcgt ctcctcgcgc cagcgcaccg gctccccgct cgaattgcgc
     3481 gtctcgatcc acatcgtcag ggtcgccttc ttcggatccc aggtcccctc ggagatccag
     3541 aaggtggtgc cggtcgagtc ggcccagagg cgccggaact tcgaagaggg ggcgtcccag
     3601 gtcacgatcg agtgcccctg gaacggccgg ccgtcgatct ggctgcggaa gtcgccggtc
     3661 acccacagcc ctccgccgcc ggtcgtgttc acctcgatgc cgttgaggat ctgcggaggc
     3721 tcgcccggcg gaccgaccac ctgcatcgtc gcgtcccagg cgccggcgaa gcgccggaac
     3781 aggtcggccg ccgccgcggg aggcgccgac ccggagggcg gggcggcggc gccgatcgag
     3841 cacaccagca gcgccggcag cagcagggca ccgaaccacc gcgacatcgt cacgctcctc
     3901 ctccgcgaaa aagggccgca ccccccgttt ccggaccggg gcggggcacg gacgctacgg
     3961 tacaatacgc gccccatgct gcgccgcccc ccctccatcc gaaagcgccg caccgtcccg
     4021 tccctgtggg ccgggatcgc cgcggcgctg ctgttcgcct cgttcgcggc accggcgcgc
     4081 gcggcgaacc acctcgcggg ggcgacgagc ccgtacctgc tggagcacgc cgacaatccg
     4141 atcgactggt atccctgggg gccgcaggcg ctcgagaagg cgaagcgcga ggacaagccg
     4201 atcttcctgt cggtcggcta cttctcctgt cactggtgcc acgtcatgga gcgggagtcg
     4261 ttcaccgacc aggagatcgc cgggctcctg aacgaagggt tcgtcgccat caaggtggat
     4321 cgcgaggagc gccccgatct cgacgccctc tacatgaacg ccgtcatcgc catgacccgc
     4381 ggaggcggct ggccgatgtc ggtcttcctg acccccgatc tgcggccgtt ccacgccggc
     4441 acctactacc cgaaggcgcg cttccgcgat ctggtgggat cgatcggcat tgcgtggcga
     4501 tcggagcggg cgcggctgct cgaggcggcc gggacgctgc gcgacgcgat ggcggagatc
     4561 cagaaaaccc ccgacgaggc gggggaggac gacccgtcga ccgacctgct ggcgctcgcg
     4621 accaaggcac tggcgaagga cttcgacgcc gagcacggcg gcttcggccg tgcgcccaag
     4681 ttcccgcccc acggcgccct cacgctcctg ctgcgcgccg atcgccaggg ggacgcgcag
     4741 gcgctgcgca tggcggccgc gaccctcgag gcgatgcggc gcggcggcat ccgcgatcag
     4801 atcggcggcg ggttccaccg ctacgcggtg gacgccgcct ggaaggtgcc gcacttcgag
     4861 aagatgctgt cggacaacgc gctgctcgcg ccgatctact tcatggcctg gaagcagacc
     4921 ggctacgggg agtttcgccg cgtcgcggag gagaccctcg ccttcgtcga gcgggagatg
     4981 tccgacccgg cgggagggta cgtcaccgcg ctcgacgccg acagcgacgg cgaggagggg
     5041 aggtactaca ccttcacggt cgacgagatc cgcgccgccg ttcccgggaa ggatgcggac
     5101 ctcgtcctcg agtattacgg cgtcacgccg aaggggagcg tggcgcgcgg gcgttcggtc
     5161 ctggccccga ccgagaagga cgaggccttc gcctcacgcc acggcctgtc gatccaggac
     5221 tggttcaccc gcctcgaggc ggcccgcaag aagctgctcc aggcgcgctc acgccgcccg
     5281 gccccgcacc gcgacgacaa ggtcctgacc gcctggaacg ggctgatgat cacggcgctc
     5341 gccagcgcgc acgtggcgac cggggacgcg gcccacctca cgcgggcgcg gcgagccgcc
     5401 gatttcgcgc tcaaggagct ggtcgggccg gacgggcggg cgcgcgtctc ctggcggcgc
     5461 ggacggcccg gggggccggg gttcctcgac gacaccgcgt tcctcatccg cgggctgctc
     5521 gacctcaacg ccaccgatcc cgacaggcgg tggatgcagg gggccacggc cctggcgaag
     5581 gacgccgcgc gcttcgccga tccgtccggc gggtggttct tcgccgccga cacccccgat
     5641 ctcatcgtcc gcccgcgcag cctggacgac tcggcccttc cggccgggaa cgccatcatg
     5701 gtggagaacc tggcgcggct ggcgcacctg tccggggatc tcggcgcgct gcaggaggcg
     5761 aagcggaccc tcgatcgcgg cgcggcggtc atgcgcaggg cgcccacagc gcacccgtat
     5821 ctcatcctgg cgcgcgagac gctgaagcag tcgcgtgccc acgggccggc cgccgacgcc
     5881 gcgtcggccc cgggtctcct gagccggccg gcgctcgccg ccgacaccgg cgcggcccgc
     5941 gccgccgctt caccggcgag cgccccggcg agcgatgccg tgagcgcggg gcaggcggcc
     6001 gccggcgccg gggccacgcg cgacgtcccg gggcaggtgg tggggcgggc caaccgcgag
     6061 cgggtggtcg agtcggttct caaggcgccg tcgcagccgg tccgtcccgg cgccgccgca
     6121 ccgctcgagt tcgtcatgac gatccgggac ggctggcaca cgaactcgtc gcgaccgacc
     6181 ctcgagtatc tgattccgac gaaagtcacc ttcaccgaca cggggagggg agcggccgag
     6241 gggatcgagt acccggacgg gaagatggtg aagctgcagt tcgccgacga gcagctctcg
     6301 gtctacgaag ggaagatcgg tatccgggcg cggctgcggg tcccgccgga cgcgccggcc
     6361 ggtccgatgg cgttcgtcgc ccggttgaac taccagtcgt gcagcgacaa ggcgtgcctg
     6421 cctcccgaga cggtcgagtt caagggcgcg gtcgaggtcg gcggagcgcc gctgagcgaa
     6481 gcggagcgcg ccgccgaagc ccccgcaccg ccctcggaag gcggtgccgg agccgcggcg
     6541 ggcggccccg cgacaggcgc aactccggga ggggcgggct ccgtcgcgtc ggcgccactc
     6601 aagggtcgcg atgggcagga tcagctgtcg ctcctcctgc gggagcgcgg catgctgttc
     6661 gtgatcgggt tcgtgttcct gggcggcctg gcgctctgtc tgaccccttg cgtctacccg
     6721 atgatcccgg tgacgctcgg gtacttcagc cagcaggccg aaggggcctc gtggggccgg
     6781 cggatcgggc tgccgtcgct gtacgtcctc ggcatcgcca tcacgtactc cgtgctcgga
     6841 gtcgtcgtcg ggctcaccgg cggactgttc ggctcgatga tgcagaaccc gatcgtcgtc
     6901 ggcgtcctga tcctcctgtt cgtcgccatg gcgttctgga tgttcggagt gtacgagctg
     6961 cggctccccg gcttcctgac ccggttcggc ggcgggcgcg ccggagcggt cggcgccttc
     7021 gtgatggggc tgacgctcgg actcgtggcg gcgccctgcg tcgggccgtt catcgtgacg
     7081 cttctggcgt tcgtggcggc gaccggcagc ccggtcatcg ggttctggct cttcttcgtc
     7141 ctggcgctcg gcatgggcct gccgttcctc gtgctcggga tcttctccgg agcgctctcg
     7201 tcgctgccgc gctcgggggc gtggctcctg tatgccaaga aggtgatggg ggtggcgctt
     7261 ctcggggtgg cgatctactt catgcagccg ttcctgagcg accgggtgct cgggtggctg
     7321 gcgatcgcgt tcgctctggg ggccggtgtt tggctcgccc tgatc
//
LOCUS       DASRIQ010000232         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_97570, whole genome shotgun sequence.
ACCESSION   DASRIQ010000232 DASRIQ010000000
VERSION     DASRIQ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97570"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFD06_05740"
     CDS             <1..407
                     /locus_tag="VFD06_05740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN03071.1"
                     /translation="GTIFVLLSRSTGRYDGLEGFGLALLEASSHGIPVIGGRTGGIPE
                     AVRHGRTGLVLPAEDARGLADEVASLLADPERRRRFGDFGRRFAAEHDWGRTAAAVRR
                     LWAGGIEAAGPPAAGSSVVGGRAERCAASPAP"
     gene            383..2356
                     /gene="asnB"
                     /locus_tag="VFD06_05745"
     CDS             383..2356
                     /gene="asnB"
                     /locus_tag="VFD06_05745"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN03072.1"
                     /translation="MCGIAGAVSLDGQPIGHDEVRAMTDALSHRGPDEGAVRLLGAGA
                     GLGRASAALGHRRLKVIDLSSAAAQPMRDAQDTGTLVYNGELYNTAELRRALLEAGRT
                     FRSRSDTEVVLQAILHWGPEEALTRFNGMFALAFWDNRERKLVLARDRFGEKPLFYAE
                     SSGRLLFASEIDALLAHGGIPDAIDPEAVELYLTFGWIPAPWTIYRAVRKLPHAAYLL
                     AGADGRVRFRRYYRLEERLQRTPPRNAPEAVRAALESAVRRRLEADVPLGAYLSGGID
                     SSAIVTFMSRLLPEPPRTYSISFPGHGYFDEGPQARLLARERSTRHTEVVLDAARLRD
                     EIPTALSAFGEPFADSSALPCSAIARVAKSGLTVALSGDAGDELFGGYRLYRALAASR
                     HVQRLPGALRSGLAQLLRALPSRHGGGVDGAVHRARKLLAGVGSELAATHAAWMSILG
                     PAARRALRPGVEDRDLGRSLVAERHRRFGGGLDRVLAVEVDLPLPDDMLAKVDRTSMR
                     HALEVRAPFLDPELVELALALPAAVHFGAASGKRLLRRSLRGIVPDRILDAPKRGFEV
                     PVGDWIAGPLAGHFGEVVTPRILEEAGGLAAATVTTWRREHAARRADHGRTLWALFVL
                     CHWHQVIRPRQQAAARRVEPLARLGLVSSSALS"
     gene            2381..2929
                     /locus_tag="VFD06_05750"
     CDS             2381..2929
                     /locus_tag="VFD06_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03073.1"
                     /translation="MRSALLVLVLMLLFIAQPAAGAADETVRPVRVINFPAVQKVQGE
                     VTIPVAVPHAALVRLRDVTVPPVEPSERGRLIDGGVVVAEGYTGVVLSLSGRAAGRAL
                     RSGAVGAYLIPDEETVMQAYEEDGLLQFPIEISAPLTQGTFRMHASQQERQTLAFPRY
                     KVRLYNTTDKTVSVNLFAYLTQ"
BASE COUNT          382 a         1141 c         1152 g          472 t
ORIGIN      
        1 tcggcacgat cttcgtcctt ctgagccgca gcaccgggcg ctacgacggc ctcgaggggt
       61 tcggcctggc gctcctcgag gcgtcgagcc acggcatccc tgtcatcggg gggcggaccg
      121 ggggcatccc ggaggcggtg cgccacggca ggaccggtct cgtcctgccg gcggaggatg
      181 cgcgcggcct ggcggacgag gtggcgagtc ttctcgccga tccggagcgg cggcggcggt
      241 tcggcgactt cggccggcgc ttcgcggcgg agcacgattg gggcaggacg gcggccgccg
      301 tgcggcgcct gtgggcgggg gggatcgagg cggcgggtcc ccccgcggcc ggttcgtccg
      361 tcgtcggagg gagggcggag cgatgtgcgg catcgccggc gccgtaagcc tcgacgggca
      421 gccgatcggc cacgacgagg tgcgcgccat gaccgacgcg ctgtcgcacc gcggcccgga
      481 cgaaggggcg gtgcggcttc tcggcgccgg cgcgggcctc ggccgtgcgt ccgcggcgct
      541 cggccaccgc cgactcaagg tgatcgacct gtcctccgcg gcggcgcagc cgatgcgcga
      601 cgcgcaggac accggcacgc tggtctacaa cggcgagctg tacaacaccg ccgagctgcg
      661 ccgggcgctc ctcgaggccg gccggacctt ccgatcccgt tccgacaccg aggtggtgct
      721 gcaggcgatc ctccactggg ggccggagga ggcgctcacc cgcttcaacg gcatgttcgc
      781 cctcgcgttc tgggacaacc gggagcggaa gctcgtcctg gcccgcgacc gcttcggtga
      841 gaagccgctc ttctacgccg agagctcggg gcggctgctg ttcgcgtcgg agatcgacgc
      901 gcttctcgcc cacggcggga tcccggacgc gatcgacccc gaggcggtgg agctgtatct
      961 gacgttcggc tggatccccg cgccgtggac gatctaccgc gcggtgcgca agctgccgca
     1021 cgcggcgtac cttctggccg gggccgacgg gcgcgtccgc ttccgccgct actaccggct
     1081 ggaggaacgg ctgcagcgga ccccgccacg gaacgcgccg gaggcggtgc gcgcggccct
     1141 cgagtcggcg gtgcgccggc gcctcgaggc ggacgtgccg ctcggcgcct atctctccgg
     1201 cgggatcgac tcgtcggcga tcgtcacctt catgtcccgc ctgctgccgg agcctccgcg
     1261 cacctactcg atctccttcc ccgggcacgg ctacttcgac gaggggccac aggcgcggct
     1321 cctggcgcgc gagcgctcca cccgccacac cgaggtggtg ctcgatgcgg cgcgcctgcg
     1381 cgacgagatc ccgaccgcgc tttccgcctt cggggagccg ttcgccgact cgagcgcgct
     1441 cccgtgctcc gccatcgccc gcgtggcgaa gagcggcctg accgtcgcgc tctccggcga
     1501 cgcgggggac gagctgttcg gcggctaccg cctctaccgt gcgctcgccg cctcgcgcca
     1561 cgtccaacgc ctcccgggag cactgcgatc gggcctggcg cagctgctcc gggcgctccc
     1621 gtcgcgccac ggcggcggcg tcgacggggc ggtgcaccgg gcgagaaagc tcctggccgg
     1681 cgtcggatcc gagctggccg cgacgcacgc cgcctggatg tcgatcctgg gccctgcggc
     1741 gcgccgcgcc ctgcggccgg gcgtcgagga tcgggatctg ggccgatccc tggtggcgga
     1801 gcggcaccgg cgcttcggcg gcgggctcga tcgggtcctg gcggtggagg tcgatctccc
     1861 tcttccggac gacatgctcg ccaaggtcga tcggaccagc atgcgccacg ccctcgaggt
     1921 gcgggccccg ttcctcgacc cggagctcgt ggagctcgcc ctggccttac cggccgccgt
     1981 ccatttcggg gccgcctccg ggaaacgcct gctgcgccgc tccctgcgcg gcatcgtgcc
     2041 ggatcggatc ctcgacgcgc cgaagcgtgg cttcgaagta ccggtcggcg actggatcgc
     2101 cggtccgctc gcggggcatt tcggcgaggt cgtcaccccg cggatcctcg aggaggccgg
     2161 cggcctcgcc gccgcgacgg tcacaacctg gcgccgcgag cacgccgccc gccgcgccga
     2221 tcacggacgg accctgtggg cgctgttcgt cctctgtcac tggcaccagg tgatccgccc
     2281 gcgacagcag gcggcggccc gccgtgtcga gccgctcgca cgattgggcc tcgtctcctc
     2341 atcagcgcta tcctgacgcc cccatttccg ggaggtctcg atgcgcagcg cgctgctggt
     2401 cctcgtcctc atgcttctct tcatcgctca gcccgccgcg ggggccgccg acgagacggt
     2461 ccgcccggtc cgggtgatca acttccccgc cgtccagaag gtgcaggggg aggtgacgat
     2521 cccggtggcg gtgccgcacg cggcgctcgt gcgcctgcgg gacgtcaccg tcccgccggt
     2581 cgaaccgtcg gagcgcggcc ggctcatcga cggcggggtc gtcgtggcgg aggggtacac
     2641 cggcgtcgtg ctgagcctga gcgggcgggc ggccggacgg gcgctccgct cgggggcggt
     2701 cggggcctac ctcatcccgg acgaggagac ggtgatgcag gcttacgagg aggatggtct
     2761 cttgcagttc ccgatcgaga tcagcgcgcc tctgacgcag gggaccttcc ggatgcatgc
     2821 gtcgcagcag gagcgccaga ccctggcctt cccgcgctac aaggtgcgcc tctacaacac
     2881 gaccgacaag acggtcagcg tcaacctgtt cgcgtatctg actcagtaag aaaatgcccg
     2941 acgagcgtgc gggctggcgg tcggaggcga acgggcggtc ggacgagtgg ccgggcccgg
     3001 tcgcgaactc cagcagattc gcgggaatct gagagtttcc tggacagtgg cgttggcact
     3061 ccacttcctc tcctcctttt cttcggagga gggttcccgt gttcgatcca ccgcgttgtc
     3121 ccaggaccac ctgtgaggcg cacgcca
//
LOCUS       DASRIQ010000233         8261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1402623, whole genome shotgun sequence.
ACCESSION   DASRIQ010000233 DASRIQ010000000
VERSION     DASRIQ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402623"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3859)
                     /locus_tag="VFD06_05755"
     CDS             complement(<1..3859)
                     /locus_tag="VFD06_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN03074.1"
                     /translation="MRPRLSRHVPGGLVWTSAAVCCLLALAGLMTRADRRQQSDRESV
                     LDQAEAQIERGLRLPTPTARPDGLSPFGTLQRLGWERSDAARTKSLDTPLGLVDPAGL
                     EDLRRQAPLLAGERGRALGRGRRGEIASGFNAVQITEDAATHRTPDEIDAELRAAGAE
                     IHGRMAERGYLVRLSDHAAGRLMDSTLVEAAMPWGPAFRVEPAIGRRPFVQRSRAQSS
                     DLRLVVEFFRGADAAQAKAEVERIAGRPAAPFAIDGQSFLVEAHHTKVPALARLPWVQ
                     WIYEEPEHLLMDSETPTTAMVGNMKDNLPFQRPYFDVGIDGGGIDTNADGQRVNNGTD
                     TVPPQIVAVTDNGISVDSAQFAQTATQPTTLASPIGPLHRKVHAIQNVTDAGDGCDGI
                     LSGGGTHGNVVAGVIAGNATALGFKISKHIYNTRPRVDGLELSGVARGARILLQDAAN
                     VTQCSLNDLIERGGNVNPGSLLTRLTLAICPRSGGVGACTGITGAGTEGHLHVMPFGV
                     PNFDTLLDNLSDGTYTAESRDIDTFLVNNRDVMVFAPVGNTGTTRVQFFFSSFNSQVR
                     NRYPDLFDGTSLDNDANIPVGLQVSPPATAKNLVSVGAHFQDVQTALSASVEENIANF
                     SAKGPATSGSLRMAPLVVGVGADLSGFFFGPNTVSAAVFKSRDNDNAGPVDAVLDDTN
                     YGTSYASAEIAGVAALIRDYLAQGFYPTGVRVDADRMPSPSGALVKAAIAASANFEEL
                     LEAEYPTPTDRTVAFSRATDLGTVSGQLIGVFGNMEQGYGRPVLTSVLPLANWPQGKG
                     VGAPQAVEYPAPGLIIYDDLGTGELAINNTTRTQIEHTFTVGSDITRLVSGTRVVDRG
                     QIRVSMAYSDPPSLAGSAGSAINDLDLELESPGADGNIATTGDNLVYDGNVYQQGGIK
                     VGQWGAARTFAAADRGDRRNPVEAVHLSADPNGDGNTNDSQLVVGTWKVRVKRGAGGA
                     TPGSISNLTGAVEDANGNGRLDAGEDTDGDTLLDADGQPYGLVISGPVFGSGPQTFNG
                     SSHSFPASRVSLDKSLYGCADEVRGSLYDAGTNAATVTGAAIFEVVNRNGVVVDSETR
                     VAFSAASGLDAYSSATVPLRESKPIAVPDNGVLETSGVVADEPYSVRLRYPDAPREVS
                     ASARISCQPYLVAWRFQTDGTNWNQQDAVFGGCDQDQNLDSGENVLYSIAFGNANRDQ
                     DYSDVSATLAVSGTGAAAVRVLNSPQNIGRLPGGQITSATFALNVDPAALNLIAVANR
                     IVDLTLTLQSSNVNVQLPRQTFTF"
     gene            4108..5553
                     /locus_tag="VFD06_05760"
     CDS             4108..5553
                     /locus_tag="VFD06_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN03075.1"
                     /translation="MFRMMKRNRDYTSPDAGDDAPFATVSDRPIDPPWRPEMEALEAA
                     FEALEGARPPLPPFAATLEWERLRSVLVEAAGRLADNFPYGHPLYVGQMLKPPHPAAR
                     LAYALAMTINPNNHALDGGRASSAMEREAVAALAALFGWSTHLGHLCGGGTMANFEAL
                     WVGRQERPGKGVAASTQAHYTHARLSEVLGVPFQGIATDPHGRIDPGALAAALQSGTV
                     GTVVATLGTTAAGAVDPLPEILDLCRARDVRVHADAAYGGYFTLAGNLDPPARAAFDR
                     IAECDSIVIDPHKHGLQPYGCGAVLFRDPAVGRHYRHDSPYTYFTGKDLHLGEISLEC
                     SRPGAAAVALWTTLRLLPLVKGGEFARGLEAGREAALQLHQAVAEDPRFAAFGPPELD
                     IVVFSRRAASASAASEANRRLFETAAAQDLHLALAAFPRHMLEGSGAVTDWDVGTLTC
                     LRACVMKPEHRAWMPEILARLRRAADAAGEP"
     gene            5604..6884
                     /locus_tag="VFD06_05765"
     CDS             5604..6884
                     /locus_tag="VFD06_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03076.1"
                     /translation="MSSPRSRGAVRLVAAACLIATTHAMAAHSAAVPAGSPAPIAPFK
                     CPGTGPNAGYAPQAMARSAERTLVLCADTEPAEGRGVVATAVAIHEPASKTPLKPIFA
                     AEEAVNRLRVEAIPDRGFALTLEALLPLGGPEYAWTPIAGFDVACGPEGCGERGARCT
                     LTLSASFKSDLTGKVRQLARGGRIEPSVAQRLLDELTVQALLGEDVAAWTVEHFDRIV
                     GIPPESKAALADARGLLARARDAGCDALVPPGDQPVVVLSQAERAAQIKEKIDQKRAG
                     KDEPAKPSAAPAAIDLQTVAWLVGGRWEGQGKMADGSALHVEETYRWGPARRSIRFQA
                     KNAGKSGAAAVEGILFFEPIPGKVILWNVKPGGGLSESAVTRADGKGMELLGPDGRVK
                     LGLQGADAQSRTVEQLQTGTWTTVATAKYQRHPR"
     gene            6881..7162
                     /locus_tag="VFD06_05770"
     CDS             6881..7162
                     /locus_tag="VFD06_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZN03077.1"
                     /translation="MSDVVEWPIDGTLDLHLFDPREIGDLVPDYLQACRERGILTVRI
                     VHGKGTGSLRSTVQAILERLPELVAERRPADETAGGWGATLVTLHPLPS"
     gene            complement(7290..8066)
                     /locus_tag="VFD06_05775"
     CDS             complement(7290..8066)
                     /locus_tag="VFD06_05775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN03078.1"
                     /translation="MGFEAQDVERARAEVRAAYSANAARVEARGFRDNPTYIATKALV
                     DWDLLDGLPLEESDVLNVGCFEPVDELHFAHRVRTWTAIDVNPDAIAVARRIVSRELA
                     PRLQERLRFQVEDGTRLSFPAASFDVVVSFSSIEHIPGAADRRAALAEMTRVLRPQGH
                     LVVTVPNRYSTFLPAHLRNRRTQSDYGYSYLYSPLELRREIRALGLELLKFSSEWHGL
                     LVMPSFMPKPLKDLLFPLVYLGERIGVLARKPGSLADPAV"
BASE COUNT         1165 a         2915 c         2957 g         1224 t
ORIGIN      
        1 ggaaggtgaa ggtctggcgc ggcagctgca cattgacgtt cgacgactgc agggtcagcg
       61 tcaggtcgac gatccggttc gccaccgcga tgaggttgag cgccgccgga tcgacattca
      121 gggcgaaggt cgcggaggtg atctgcccgc ccggcagccg gccgatgttc tgcggcgaat
      181 tgaggacgcg caccgcggcg gcccccgtgc cgctcaccgc cagggtcgcg ctcacgtcgc
      241 tgtagtcctg gtcgcggttg gcgttgccga aggcgatcga gtacagcacg ttctcgccgg
      301 agtccaggtt ctggtcctgg tcgcagcctc cgaacaccgc gtcctgctgg ttccagttgg
      361 tgccgtcggt ctggaagcgc caggccacga ggtacggctg gcaggagatg cgggccgacg
      421 cggagacctc gcgcggcgcg tccggatagc gcagccggac cgagtacggc tcgtcggcga
      481 ccacgcccga ggtctcgagc acgccgttgt ccggcacggc gatcggcttc gactcgcgca
      541 gcggcaccgt cgcggaggag taggcatcga gccccgaggc agcgctgaac gccacgcgcg
      601 tctcgctgtc caccacgacg ccgttgcggt tgaccacctc gaagatcgcg gcgccggtga
      661 cggtcgcggc gttggtgccg gcgtcgtaca ggctgccgcg cacctcgtcg gcgcagccgt
      721 acaggctctt gtcgaggctg acgcggctgg ccgggaacga gtggctggac ccgttgaagg
      781 tctgcgggcc gcttccgaag accgggcccg aaatcaccag cccgtatggc tggccgtcgg
      841 cgtcgagcag cgtgtcgccg tcggtgtcct cccccgcgtc gaggcggccg ttgccgttgg
      901 cgtcctccac cgccccggtg aggttgctga tgctgccggg ggtggcgccg ccggcgcccc
      961 gcttcacgcg caccttccag gtcccgacca cgagctgcga atcgttggtg ttgccgtcgc
     1021 cgttcggatc ggcggagagg tgcaccgcct cgaccgggtt gcggcgatcg ccgcgatcgg
     1081 ccgcggcgaa ggtccgcgcc gccccccact ggccgacctt gatccccccc tgctgataga
     1141 cgttgccgtc gtagacgagg ttgtcgccgg tggtggcgat gttgccgtcg gcgccgggag
     1201 actccagctc gagatcgagg tcgttgatcg ccgacccggc gctgccggcc agcgacggcg
     1261 ggtcggagta ggccatcgag acccggatct ggccgcggtc cacgacgcgg gttcccgaca
     1321 cgagccgcgt gatgtccgat ccgaccgtga aggtgtgctc gatctgcgtc cgcgtggtgt
     1381 tgttgatcgc cagctcgccg gtccccagat cgtcatagat gatgagcccc ggggccgggt
     1441 actccaccgc ctgcggagcg ccgaccccct tcccctgcgg ccagttggcc agcggcagca
     1501 ccgaggtcag gaccggccgg ccgtacccct gctccatgtt gccgaagacg ccgatgagct
     1561 ggcccgacac cgtcccgagg tcggtggcgc ggctgaaggc gacggtgcga tccgtcggcg
     1621 tcgggtactc cgcctccagg agctcctcga agttggcgga ggcggcgatc gccgccttca
     1681 ccagcgctcc ggacggcgac ggcatccggt cggcgtccac cctcacgccg gtcgggtaga
     1741 acccctgcgc caggtagtcg cggatgaggg ccgcgacgcc ggcgatctcc gccgaggcgt
     1801 aggaggtgcc gtagttggtg tcgtcgagca ccgcgtccac cggcccggcg ttgtcgttgt
     1861 cgcggctctt gaagaccgcc gccgagacgg tgttcggccc gaagaagaag ccgctcagat
     1921 cggcgccgac gcccaccacc aggggcgcca tgcgcagcga gccggaggtg gccggcccct
     1981 tggcggagaa gttggcgatg ttctcctcga cgctcgccga cagcgccgtc tgcacgtcct
     2041 ggaaatgggc gccgaccgag acgaggttct tggcggtcgc cggcgggctc acctggagcc
     2101 cgaccgggat gttggcgtcg ttgtcgaggg aggtgccgtc gaacaggtcc ggatagcggt
     2161 tgcgcacctg gctgttgaac gacgagaaga agaactgcac gcgcgtcgtc ccggtgttgc
     2221 cgaccggcgc gaacaccatc acgtcgcggt tgttcaccag gaaggtgtcg atgtcgcgcg
     2281 attccgccgt gtaggtcccg tcgctgaggt tgtcgaggag ggtgtcgaag ttcggcacgc
     2341 cgaacggcat cacgtgcagg tgcccctcgg ttcccgcgcc ggtgatgccg gtgcacgccc
     2401 cgacgccgcc ggagcgcggg cagatcgcca gggtcagccg cgtcaggagg gatcccggat
     2461 tgacgttccc cccccgctcg atgaggtcgt tcagcgagca ctgggtgacg ttggcggcgt
     2521 cctgcagcag gatgcgggcg ccgcgcgcca cgccgctgag ctccagcccg tcgacgcgcg
     2581 ggcgggtgtt gtagatgtgc ttgctgatct tgaagccgag cgccgtggcg ttcccggcga
     2641 tgaccccggc cacgacgttg ccgtgcgtgc ctccgccgga gaggatgccg tcgcagccgt
     2701 cgccggcgtc ggtcacgttc tggatggcgt ggaccttccg atgcaacggg ccgatggggc
     2761 tcgcgagcgt cgttggctgg gtggcggtct gcgcgaactg cgccgagtcg accgagatgc
     2821 cgttgtcggt caccgcgacg atctgcggcg gcaccgtgtc ggtgccgttg ttgacccgct
     2881 ggccgtcggc gttcgtgtcg atcccgccgc cgtcgatgcc gacgtcgaag taggggcgct
     2941 ggaacggcag gttgtccttc atattgccga ccatcgccgt ggtcggcgtc tcggagtcca
     3001 tgagcaggtg ctccggctcc tcgtagatcc actgcaccca ggggaggcgg gcgagggccg
     3061 ggaccttcgt gtggtgcgcc tcgacgagga acgactggcc gtcgatcgca aacggcgccg
     3121 ccggccgacc ggcgatccgc tcgacctccg ccttcgcctg cgccgcgtcg gcgccgcgga
     3181 agaactcgac gacgagcctc aaatcggagg actgggcgcg cgagcgctgc acgaaggggc
     3241 gccggccgat cgccggctcg acgcggaagg cggggcccca cggcatcgcc gcctcgacga
     3301 gcgttgagtc catcagccgt ccggccgcgt ggtcgctgag gcgcacgagg tagccgcgct
     3361 ccgccatccg tccgtggatc tccgcccccg cggcgcgcag ctccgcgtcg atctcgtccg
     3421 gggtcctgtg cgtcgccgcg tcctcggtga tctgcacggc gttgaagccg gaggcgatct
     3481 ctccgcggcg cccgcggccc agcgcgcgcc cgcgctcccc cgcgaggagc ggggcctggc
     3541 ggcgcaggtc ctcgagcccg gccggatcga cgagcccgag cggggtgtcg aggctcttcg
     3601 tgcgcgccgc gtcgctcctc tcccacccga gacgctgcag cgtgccgaac ggcgagaggc
     3661 cgtccggccg ggcggtcggg gtcggcagcc gcaggcccct ctcgatctgg gcctcggcct
     3721 gatccagcac cgactcgcga tccgactgct ggcggcggtc ggcgcgagtc atgaggccgg
     3781 cgagtgcgag cagacagcag acggcggcgc tggtccagac caggccgcca ggaacgtggc
     3841 gcgaaaggcg aggacgcatg gactcctccc tctcctccct cacgggaagt cgaatggaat
     3901 gggactcgac ctggcgagaa cggcgattct caggtcagga atgacgacac tcctgcacgc
     3961 gggccggcgc acatgggtcc cgtccggacc cgcacgccgg cacatggcgt tcatgtacga
     4021 cgaccgggag ggggagtcaa cctgcgaacg accgattccg ccgatctccg ggaagggccg
     4081 gccgtcaagg agatgccgcg gggggcgatg ttccgcatga tgaagagaaa ccgggactac
     4141 acttcccccg atgccggcga cgacgccccc ttcgcgaccg tgagcgaccg accgatcgac
     4201 cctccctggc ggcccgagat ggaggcgctc gaggccgcct tcgaagcgct cgagggcgcc
     4261 cgcccgccgc tcccaccgtt cgccgcgacg ctcgaatggg agcggctgcg ctccgtcctg
     4321 gtcgaggcgg ccgggcggct cgccgacaat tttccgtacg gccacccgct ctacgtcggg
     4381 cagatgctca agccgcccca tccggcggcg cgcctcgcct acgccctggc gatgaccatc
     4441 aacccgaaca accacgccct cgacggggga cgcgcctcct ccgccatgga gcgggaggcg
     4501 gtggccgcgc tcgccgccct gttcgggtgg tccacccacc tcggccacct gtgcgggggg
     4561 gggacgatgg ccaacttcga ggccctgtgg gtcggacggc aggaacgtcc cggaaagggg
     4621 gtcgcggcct ccacgcaggc gcactacacc catgcccgcc tgtcggaggt gctgggggtg
     4681 ccgttccagg ggatcgccac cgatccgcac gggcggatcg atccgggcgc cctcgccgcg
     4741 gcccttcaga gcgggaccgt cggcaccgtg gtcgcgacgc tcgggaccac cgccgccggg
     4801 gcggtcgatc ccctgccgga gatcctcgat ctctgccgcg cccgcgacgt ccgggtgcat
     4861 gccgacgccg cctacggcgg ttacttcacc ctcgccggca acctcgaccc tccggcgcga
     4921 gccgccttcg accggatcgc cgagtgcgat tcgatcgtca tcgacccgca caagcacggg
     4981 ctgcagccgt acggatgcgg cgcggtgctc ttccgcgatc cggcggtcgg ccgccactac
     5041 cggcacgact ccccgtacac ctacttcacc gggaaggatc tgcacctcgg cgagatctcg
     5101 ctcgagtgct cccgccccgg ggccgcggcg gtggcgctct ggaccaccct ccgcctgctg
     5161 ccgctcgtga agggggggga gttcgcgcgc ggcctcgagg cgggacgcga agcggcactg
     5221 cagctccacc aggcggtggc cgaagacccc cgcttcgccg cgttcgggcc gcccgagctc
     5281 gacatcgtcg tgttctcgcg ccgggccgcc tcggcctcgg ccgcctcgga ggcgaaccgg
     5341 cggctcttcg agaccgccgc cgcgcaggac ctccacctcg cgctggccgc cttcccccgc
     5401 cacatgctgg agggttcggg ggcggtcacg gactgggacg tcgggaccct gacctgcctg
     5461 cgcgcctgcg tcatgaagcc ggagcatcgc gcctggatgc cggagatcct cgcccgcctg
     5521 cggcgggccg ccgacgccgc cggggaaccg tgaaccggcg agtccgcctt gactctccgc
     5581 aaaggggagt cgatcatccc tccatgtctt cgccccgatc gcgcggcgcc gtccggctcg
     5641 tggcggcggc ctgtctgatc gcgacgacgc atgccatggc ggcacactcc gccgcggtcc
     5701 ctgccggctc ccccgccccg atcgccccgt tcaagtgccc gggcaccggc ccgaacgccg
     5761 ggtacgcgcc gcaggcgatg gcccgctcgg ccgaacggac gctcgtgctg tgcgccgaca
     5821 ccgagccggc ggaagggcgc ggcgtcgtcg cgaccgcggt cgccatccac gagccggcat
     5881 cgaagactcc tctcaagccg atcttcgcgg cggaggaggc cgtcaaccgc ctgcgcgtcg
     5941 aagcgatccc ggatcgcggc ttcgctctga ccctcgaggc gcttctgccg ctcggcggac
     6001 cggagtacgc gtggactccg atcgccggct tcgacgtcgc ctgtggtccg gagggatgcg
     6061 gcgagcgcgg agcgcggtgc accttgaccc tgtccgcgtc cttcaagagc gacctgacgg
     6121 ggaaggtgcg gcagctggcg cgcggcgggc gcatcgagcc gagcgtggcg cagcggctcc
     6181 tcgacgagct caccgtccag gcgcttctcg gcgaggacgt cgccgcctgg accgtcgagc
     6241 acttcgatcg gatcgtgggg atcccgcccg agagcaaggc ggcgctcgcg gacgcgcgcg
     6301 gcctgctggc gcgggcgcgc gacgccggct gcgacgcgct ggtcccgccc ggggatcagc
     6361 cggtcgtcgt gttgagccag gccgagcggg cggcgcagat caaggaaaag atcgaccaga
     6421 agcgcgcggg gaaggacgag ccggccaagc cttccgccgc acccgccgcc atcgatctcc
     6481 agacggtcgc ctggctcgtc ggcggccgct gggaagggca ggggaagatg gccgacggca
     6541 gcgcgctgca cgtcgaggag acctatcgct gggggccggc ccggagatcg atccgcttcc
     6601 aggcgaagaa tgccggcaag tccggcgcgg ccgcggtcga agggattctc ttcttcgagc
     6661 cgatccccgg gaaggtgatc ctctggaacg tcaagcccgg cggcgggttg agcgagagcg
     6721 ccgtcacccg ggccgacggc aaaggcatgg agctgctcgg ccccgacggg cgcgtcaagc
     6781 tggggctcca gggcgccgac gcgcagagcc ggacggtgga gcagctgcag acggggacct
     6841 ggacgaccgt cgccaccgcc aagtatcagc ggcacccccg ttgagcgacg tcgtcgagtg
     6901 gccgatcgac ggcacgctcg acctgcacct gttcgacccg cgcgagatcg gcgatctcgt
     6961 cccggactat ctccaagcct gccgggagcg gggcatcctc acggtccgca tcgtccacgg
     7021 caaggggacc ggttcactgc gcagcaccgt ccaggcgatc ctggagcgcc tcccggagct
     7081 ggtcgccgag cggcggccgg ccgatgagac cgccgggggg tggggggcga cgctcgtcac
     7141 gctccatccc ctgccgagct gatctctacg ccggccgact ctttcgcatc acgctcgcac
     7201 accgtcgaat gaatttcgaa ggcggcccgt tgttcaccca gccgcccgcg attttgaaga
     7261 gagtgggggg cgtgccggcg tcaggaccct cagaccgccg ggtccgccag cgatccgggc
     7321 ttgcgagcca gaacgccgat gcgctcgccc aagtagacca gcggaaagag gagatccttg
     7381 agcggcttcg gcatgaagga gggcatgacc agaaggccgt gccactccga tgagaacttc
     7441 aggagttcca gtcccagggc gcggatctcg cgtctcaact ccagcgggct gtagaggtag
     7501 gagtatccgt agtcgctttg tgtgcgccgg ttgcgcaggt gcgccggcaa gaaggtgctg
     7561 tagcggttgg gcaccgtgac gacgagatgt ccctgcggcc gtagcacgcg ggtcatctcc
     7621 gcgagcgccg cacggcgatc ggcggcgccc gggatgtgct cgatggatga gaatgacacc
     7681 accacgtcga aggaggcggc cgggaacgac aggcgcgtcc cgtcctcgac ctggaatcgc
     7741 aggcgctcct ggagccgggg cgccagttcc cggctgacga tccgccgggc gaccgcgatc
     7801 gcgtccgggt tgacgtcgat ggcggtccaa gtccgtacgc ggtgagcgaa gtgcagttcg
     7861 tcgaccggct cgaagcagcc aacgttgagc acgtcgcttt cctcgagcgg gagaccgtcc
     7921 aggaggtccc agtcgaccag ggctttggtc gcgatgtagg tggggttgtc cctgaaccct
     7981 ctggcctcga cgcgggccgc gttggcgctg taggccgcgc ggacctcggc tcgggcccgc
     8041 tccacatcct gcgcttcgaa gcccatcccc gcctttcccg cccggccgcc atcgggcgca
     8101 caggaccctc ggccaacgta gccgctacgt caccaaccta ggtgctgcgt cacggggtgt
     8161 caaccttccg acacaattcg cagcccggcg gtccctcagc caagtctggc tccggtccgc
     8221 acaggtcggt cgtgatccaa cccactgccg ggcgctaggt t
//
LOCUS       DASRIQ010000234         5597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1154941, whole genome shotgun sequence.
ACCESSION   DASRIQ010000234 DASRIQ010000000
VERSION     DASRIQ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154941"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFD06_05780"
     CDS             complement(<1..624)
                     /locus_tag="VFD06_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN03079.1"
                     /translation="MRPALSEAGARLRIACAAVLFSTGGAAIKWIELGGWQVAALRSA
                     IAVPAVLLLLPEARRRPRPAVLVLGVFYAATLVCFVLATKWTTAAAAIFLQSTAPIYV
                     VLLSPRLLGEPVRRSDVAFLLPLAAGLILLLAGGAPVQATAPRPVLGNLLAALSGLTW
                     ALTLIGLRRAGREGRDDAPSAVVVGNAFACLMTLPWALPLAGIGAPDL"
     gene            complement(631..1257)
                     /locus_tag="VFD06_05785"
     CDS             complement(631..1257)
                     /locus_tag="VFD06_05785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZN03080.1"
                     /translation="MNIVDSRLDEYLDRLVPERPPVVREMETLAQRRHFPIVGPQVGR
                     LLHLLARGIGAKDILECGSGFGYSAWWFVTAIPEGGRVVMSEGGAENCEQARDFLGRA
                     GMGERIALHHANALDVLERLPGPFDIVFCDIDKRDYPRVYPFLATRLRRGGLFICDNM
                     LWFGRVAGPDEDPDTRGVRELTRLLYEDPAFVTTILPVRDGVGVSLKI"
     gene            complement(1287..1808)
                     /locus_tag="VFD06_05790"
     CDS             complement(1287..1808)
                     /locus_tag="VFD06_05790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZN03081.1"
                     /translation="MAIGAQHFRDTLRLWASGVTVVTARAPRGLVAITVSSFASLSLR
                     PPLVLVCIERRARAHRALARARAFGVNLLRVDQEELSDRAAGRLGRRAARLPRARLRQ
                     AVTGAPILKDALAWLDCTLVARHRGGDHTIFVGRVEAAGTGRGRPLLWFDRDYAALRR
                     KSSRAPLRRAARL"
     gene            1915..2586
                     /locus_tag="VFD06_05795"
     CDS             1915..2586
                     /locus_tag="VFD06_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN03082.1"
                     /translation="MIRGARIESLYLAGPAGRLEAVLKHPPVPASPAGDDDARADPPA
                     VAVVCHPHPLFGGTLHNKVVHAAAEALARRGLPTLRFNFRGVGRSAGRHDNGRGEQED
                     LGAALAFLEERYPGRKILAAGYSFGAFVALAVGCADERVVSLVAIAAPVGVYPFGFLR
                     ACRKPLVLIQGEADPLAPLPLALALAAALPAGARVVPIPGADHAFRGRLDLVGQRVEE
                     VAASL"
     gene            2680..3006
                     /locus_tag="VFD06_05800"
     CDS             2680..3006
                     /locus_tag="VFD06_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZN03083.1"
                     /translation="MRAQRLTAAAAVILAAAVLPLLAQDDPKIARGKEVYAAQKCSMC
                     HMVEGKGNKNAPLDGVGSKLKPEEIRKWVTAPKEMQPNSKMKAYPNLPPADLDALIAY
                     LSSLKK"
     gene            3021..4466
                     /locus_tag="VFD06_05805"
     CDS             3021..4466
                     /locus_tag="VFD06_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN03084.1"
                     /translation="MTSPARRRSPPALQACLRVVFTIRLRPAFALALAVAAGLTAPAI
                     TVAESPAPPLVLRAARLLDGTGSDLRRNVAVVIKDGRIERVAAAGGFQPPPGASTIDL
                     GDVTLVPGLIDCHVHLTMEITRSWEFESVKGTPADAALLGAVSAMKTLRAGFTTVRNL
                     GDRGGESVALRNAIDRGLLPGPRVLTARRMLSITGGHGDWSGGFRPGLSLLGLDPPEA
                     GLCDSPDACRAAVRTQVKYGADVIKISATGGVLSSGDEIGARQFSDEELKAIVSEAHL
                     LGRKVAAHAHGTSGIKAAVLAGVDSIEHGSILDDETIRLMKERGTVLVPTLMAGETVE
                     AAARAGTLPDFAVSKALAVRPKMVSSFRRAVAAGVRIAFGTDSSVSPHGKNAHEFELM
                     VQGGMTPGAALKAATSTAAALLGREAEIGTITEGKAADLVAVPGDPLRDVSVLLRPVA
                     VFQAGREVDLGAAPAPRTASPSAPAASSSQR"
     gene            4463..5011
                     /locus_tag="VFD06_05810"
     CDS             4463..5011
                     /locus_tag="VFD06_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HZN03085.1"
                     /translation="MNAATGKPRVGVSSCLLGEAVRWNGAHKHDPNLTEALGRFFDWV
                     AVCPEVELGLGVPREPIRLEGDPASPRLVGRTTGIDLTDRMEAFAAERVEQLASFDLC
                     GYVLKSDSPTCGMERVEVSGAPPDGPQSGAGLFARALMRRLPLLPIEEEGRLRDPGVR
                     ETFIARVHTYRTRATRRLRNHA"
     gene            5004..>5597
                     /locus_tag="VFD06_05815"
     CDS             5004..>5597
                     /locus_tag="VFD06_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN03086.1"
                     /translation="MPEAHAPFGRIGFGGYRVDEGIPAHRQALERALERGCTLIDTST
                     NYTDGGSESLVGEVLAERERRDPGSRGRVVVVSKIGYVQGRNLDLAMERERRGEPFPE
                     MVRYMDGCWHCIHPEFLRDQLRRSLERLQADRLDVCLLHNPEYFLSDAAHRGDARDLE
                     ATRAEFYRRVQAAFAFFEEQVKSGLLGAYGVSSNSVVA"
BASE COUNT          798 a         2019 c         2051 g          729 t
ORIGIN      
        1 gagatccggt gcgccgatcc ccgcgagcgg cagcgcccac ggcagcgtca tcaggcacgc
       61 gaaggcgttg ccgaccacga ccgcggacgg ggcgtcgtcc cgcccctccc gcccggcgcg
      121 ccgcagtccg atgagggtga gcgcccaggt caggccgctg agggcggcga gcaggttgcc
      181 gaggaccgga cgcggggcgg tcgcctgaac cggcgccccg ccggcgagga gcaggatcag
      241 cccggccgcg agcggcagga ggaacgcgac gtcggatcgc cgcaccggct cgccgaggag
      301 ccgcggcgag aggagcacga cgtagatcgg cgccgtggat tgcaggaaga tggcggccgc
      361 ggcggtggtc cacttcgtgg cgaggacgaa gcagacgagg gtcgcggcgt agaacacccc
      421 gaggacgagc accgccgggc gggggcgccg gcgcgcctcc ggcagcagga ggagcaccgc
      481 cggcaccgcg atcgccgatc gcagcgccgc gacctgccag ccgccgagct cgatccactt
      541 gatggccgct ccgccggtgg agaacagcac cgcggcgcag gcgatccgca gtcgcgcccc
      601 cgcttcgctc agggccgggc gcaagagaat ctagatcttg agggagacgc cgaccccgtc
      661 gcgcaccgga aggatggtgg tcacgaacgc ggggtcctcg tacagcaggc gggtcagttc
      721 gcgcacgccg cgggtgtcgg ggtcttcgtc cggaccggcc acccggccga accacagcat
      781 gttgtcgcag atgaagagcc cgccgcggcg cagccgcgtg gcgaggaacg ggtagacgcg
      841 cggatagtcc cgcttgtcga tgtcgcagaa gacgatgtcg aacggccccg ggaggcgctc
      901 gaggacgtcc agggcgttgg cgtggtgcag ggcgatccgc tcccccatgc cggcgcggcc
      961 gaggaagtcg cgcgcctgct cgcagttctc cgccccgccc tcgctcatca ccacgcgtcc
     1021 cccctcgggg atcgcggtga cgaaccacca ggcggagtag ccgaagccgg agccgcactc
     1081 gaggatgtcc ttcgccccga tcccgcgcgc cagcaggtgc aggagacggc ccacctgggg
     1141 gccgacgatc ggaaagtggc ggcgctgcgc cagcgtctcc atctcgcgca cgaccggggg
     1201 acgctcgggc accagacgat cgaggtactc gtccaggcgg gagtcgacga tattcacggt
     1261 cggatcgtac cggatcgccc cgtgactcac aagcgcgccg cgcggcgcag aggcgcccgg
     1321 gaggacttcc ggcgcagcgc cgcgtagtcg cggtcgaacc acaggagcgg cctgccgcgg
     1381 ccggtcccgg ccgcctcgac ccgcccgacg aagatggtgt gatcgccgcc gcgatggcgg
     1441 gcgacgagcg tgcagtcaag ccacgcgagc gcgtccttga ggatcggggc gccggtgacc
     1501 gcctgccgca gccgcgcgcg cggcagccgc gccgcacggc ggccgagccg gcccgcggcc
     1561 cggtccgaca gctcctcctg gtccactctc aggagattga ctccgaaggc acgcgcccgg
     1621 gcgagggccc gatgggcgcg ggcgcgtcgc tcgatgcaga cgagcacgag cggcggccgg
     1681 agcgacagcg acgcgaacga cgacacggtg atcgccacga gaccgcgcgg cgcgcgcgcc
     1741 gtcacgacgg tgacgccgct ggcccagagc ctgagcgtgt cccggaaatg ctgcgccccg
     1801 atcgccaacg acgtgccgct ccccctccgg gtaactttcc agcgggcgag attacactag
     1861 gagcctgtcc gagtcttcgc aaggatcgcg ttcgcggcga acctcggaac catcgtgatc
     1921 cgcggcgcgc gcatcgaatc gctgtatctc gccggcccgg ccgggcggct ggaggcggtg
     1981 ctcaagcacc cgccggtccc ggcctcccct gccggcgacg acgacgcccg ggccgacccg
     2041 ccggccgtcg cggtcgtctg ccacccgcac ccgctgttcg gcggcaccct gcacaacaag
     2101 gtggtgcacg cggccgccga ggcgctggcg cggcgcgggc ttccgaccct gcgcttcaac
     2161 ttccgcggcg tcgggcggag cgccggccgt cacgacaacg gccgcggcga gcaggaggat
     2221 ctgggggcgg ctctcgcctt tctcgaggaa cgctatcccg gccggaagat cctcgccgcg
     2281 gggtactcgt tcggggcctt cgtcgccctc gccgtcggct gcgccgacga gcgggtcgtc
     2341 tcgctcgtcg cgatcgccgc cccggtgggg gtgtacccct tcgggttcct gcgcgcctgc
     2401 cgcaaaccgc tggtgctcat ccagggagag gcggacccgc tcgcgccgct ccccctggcg
     2461 ctcgcgctgg ccgccgccct ccccgcgggg gcccgggtgg tgccgatccc cggggcggac
     2521 cacgccttcc gcggccgcct cgacctggtc gggcagcggg tcgaggaggt ggcggcctcg
     2581 ctgtgagggc cggcgcggcg ggcgcggcgc gggaccaccg aggtgtgccg gggggaggag
     2641 aaggtgatat cgttccggct catctccgag gagagcccca tgagagccca gcgtctgaca
     2701 gcagccgccg cggtcatcct ggccgccgcg gtccttcccc tcctggcgca ggacgatccc
     2761 aagatcgccc gcgggaagga agtctatgcg gcgcagaagt gctcgatgtg ccacatggtc
     2821 gaggggaagg ggaacaagaa cgccccgctc gacggtgtcg gcagcaagct gaagccggag
     2881 gagatccgca agtgggtcac cgcccccaag gagatgcagc cgaactccaa gatgaaggcg
     2941 tacccgaatc ttccgccggc cgacctcgac gcgctgatcg cctacctctc cagcctcaag
     3001 aagtaaggac caggagcctc gtgacgtcgc cggcgcgccg gagatcaccg ccggcgcttc
     3061 aggcctgcct gcgcgtcgtg ttcacgatcc gtctccggcc ggcgttcgcg ttggcgctcg
     3121 cggtggccgc gggcttgacc gcgccggcca tcaccgtggc ggaatcgccg gccccgccgc
     3181 tcgtcctgcg cgccgcgcgc ctgctcgacg gcacaggctc cgacctccgc cgcaacgtcg
     3241 cggtcgtgat caaggatggc cggatcgagc gcgtggccgc ggcgggtggg ttccagcctc
     3301 cgcccggggc cagcacgatc gatctcggcg acgtcaccct cgtccccggg ctcatcgact
     3361 gccacgtcca cctcacgatg gaaatcaccc gctcctggga attcgagtcg gtgaaaggga
     3421 cgccggccga cgcggcgctt ctgggggcgg tcagtgccat gaagaccctg cgcgccggtt
     3481 tcacgacggt gcgcaacctc ggcgaccgcg gaggggagag cgtggcgctg cgcaacgcga
     3541 tcgaccgcgg gctcctcccc ggaccccgcg tcctcaccgc ccggcgcatg ctgtcgatca
     3601 ccggcggcca cggcgactgg agcggcggct tccggcccgg gctgtcgctt ctcggcctcg
     3661 acccgccgga ggccgggctg tgcgactccc ccgacgcctg ccgggcggcg gtgcgcaccc
     3721 aggtgaagta cggcgcggac gtcatcaaga tctccgccac cggcggggtg ctctcgtccg
     3781 gagacgagat cggcgcgcgg cagttcagcg acgaggagct caaggcgatc gtctccgagg
     3841 cgcacctgct cggccgcaag gtggcggcgc acgcccacgg cacctccggg atcaaggcgg
     3901 cggtgctggc aggcgtcgac tccatcgagc acgggtcgat cctggacgac gagacgatcc
     3961 ggctgatgaa ggagcgcggc acggtgctcg tgccgacgct gatggccggc gagactgtgg
     4021 aggccgccgc gcgcgcgggc acgctccccg attttgccgt gtccaaggcg ctcgcggtgc
     4081 ggccgaagat ggtcagctcc ttccggcggg cggtcgcggc cggcgtgcgg atcgccttcg
     4141 gtaccgacag ctccgtctcc ccgcacggga agaatgcgca cgagttcgag ctgatggtcc
     4201 agggggggat gactccgggc gcggcgctca aggcggcgac cagcaccgcc gccgcgcttc
     4261 tggggcgcga ggcggagatc ggcacgatca ccgaggggaa ggcggccgac ctcgtcgccg
     4321 tccccggcga tccgctgcgc gacgtgagcg tccttctgcg tccggtcgcc gtgttccagg
     4381 cgggacgcga agtggacctg ggggccgccc ccgcgccgcg gacggcgtct ccctcggccc
     4441 cggcggcttc ctcgtcgcag cgatgaatgc cgcgacgggg aaaccgcgcg tcggcgtctc
     4501 ctcgtgcctc ctgggcgaag cggtccgctg gaacggggcc cacaagcacg acccgaacct
     4561 gacggaggcc ctcggccgtt tcttcgactg ggtcgcggtc tgcccggagg tggagctcgg
     4621 cctcggagtg ccgcgcgaac cgatccgcct cgaaggcgat ccggcttcgc cgcggctcgt
     4681 cgggcggacg acggggatcg atctcaccga tcgcatggag gcgttcgccg cggagcgcgt
     4741 cgagcagctc gcgtccttcg acctgtgcgg ctatgtcctg aagtccgatt cgccgacctg
     4801 cgggatggaa cgggtggagg tgtccggtgc gccccccgac gggccgcagt cgggagccgg
     4861 tcttttcgcg cgcgcgctga tgcggcggct gccgctgctg ccgatcgagg aggaggggcg
     4921 actccgcgac cccggcgtgc gcgagacctt catcgcgcgg gtgcacacct accggacccg
     4981 ggcgacacgg aggctccgga accatgcctg aagcccacgc accgttcggc cggatcggct
     5041 tcggcggcta ccgcgtcgac gaagggattc ccgcgcaccg ccaggcgctc gagcgcgccc
     5101 tcgagcgcgg ctgcaccctc atcgacacct ccaccaacta caccgacggc ggcagcgagt
     5161 cgctcgtggg ggaggtgctc gcggagcggg agcggcgcga tcccggctca cgcgggcggg
     5221 tcgtcgtggt gtcgaagatc ggctacgtgc agggccggaa cctcgacctg gcgatggagc
     5281 gcgagcgccg gggcgagccg ttcccggaga tggtgcgcta catggacggc tgctggcact
     5341 gcatccaccc cgagtttctg cgggaccagc tgcgccgttc tctcgagcgg ctccaagccg
     5401 atcgtctcga tgtctgcctg ctgcacaacc cggaatactt cctgtccgac gcggcgcacc
     5461 gtggcgacgc ccgcgatctc gaggcgacgc gcgccgagtt ctatcgccgc gtgcaggcgg
     5521 ccttcgcctt cttcgaagaa caggtcaaga gcggcctcct cggcgcctac ggcgtctcct
     5581 cgaacagcgt ggtggcg
//
LOCUS       DASRIQ010000235         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_622047, whole genome shotgun sequence.
ACCESSION   DASRIQ010000235 DASRIQ010000000
VERSION     DASRIQ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622047"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VFD06_05820"
     CDS             complement(<1..747)
                     /locus_tag="VFD06_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HZN03087.1"
                     /translation="MTAEEYERAVQLLGRVPTWTELGIIAVMWSEHCSYKSSKIHLKR
                     LPTTGPRMVQGPGENAGVVDIGDGIVAVFKMESHNHPSFVEPYQGAATGVGGILRDIF
                     TMGARPIASLDSLRFGALTAPRMKYLVGGVVAGIAGYGNCMGVPTVGGDLAFHPSYNN
                     NILVNVFNLGIARPDKIHKGAAKGNKNPVMYVGSRTGRDGIHGASLLASAEFDAESES
                     KRPTVQVGDPFTEKLLLEACIEVMKEKGLVV"
     gene            complement(830..1540)
                     /gene="purQ"
                     /locus_tag="VFD06_05825"
     CDS             complement(830..1540)
                     /gene="purQ"
                     /locus_tag="VFD06_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZN03088.1"
                     /translation="MKFGIVVFPGSNCEHDVYHVVKHLLGHEATFIWHKDESLAGIDC
                     IVLPGGFAHGDYLRTGAMARFSPIMKSIKQYADRGGMVFGICNGFQILAEAGLLPGAL
                     VRNTNLQYICRDLYLRVERQDTAYTNRIKQGSVLKIPIGHGEGCYYAPDAVLQELAKK
                     RQVVFRYCDPKGEITAEANPNGSLQAIAGICNEKGNVLGMMPHPDRCAEEVLGNADGL
                     RLFTSILHTWQQTRRNRI"
     gene            complement(1548..1793)
                     /gene="purS"
                     /locus_tag="VFD06_05830"
     CDS             complement(1548..1793)
                     /gene="purS"
                     /locus_tag="VFD06_05830"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HZN03089.1"
                     /translation="MKARVYVTLKRGVLDPQGKAVCTSLHQLGYREVTDVRIGKFMEV
                     NLGGLPREEAEKRLGDMCQKLLANTVIEDYRFEFLDE"
     gene            complement(1863..>2205)
                     /locus_tag="VFD06_05835"
     CDS             complement(1863..>2205)
                     /locus_tag="VFD06_05835"
                     /inference="COORDINATES: protein motif:HMM:NF021883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZN03090.1"
                     /translation="LDYMTARFTRIVNGMVVNAAAMQRNLEASGGLIYSGNVLLALAE
                     RGVTRDQAYEWVQAAAMQARSGGGDFRALLKGNPEVTRVLPVAEIDRCFDLGHQLRHV
                     ETLFKRVFGPA"
BASE COUNT          327 a          760 c          710 g          408 t
ORIGIN      
        1 gacgacgagc cccttctcct tcatcacttc gatgcacgcc tcgaggagga gcttctccgt
       61 gaacgggtcg ccgacctgaa cggttggacg cttgctctcc gattcggcat cgaactccgc
      121 cgacgccagc aggctcgccc cgtggatgcc gtcgcgcccg gtgcgcgagc cgacgtacat
      181 caccgggttc ttgtttccct tcgccgcccc cttgtgaatc ttgtcggggc gcgcgatccc
      241 gaggttgaag acgttcacca ggatgttgtt gttgtacgac gggtggaacg ccaggtcgcc
      301 gccgaccgtc gggacgccca tgcagttgcc gtagccggcg atgcccgcga cgacgccgcc
      361 gacgaggtat ttcatgcggg gggcggtgag ggcgccgaat ctcagggagt cgagggaggc
      421 gatcggtctg gcccccatcg tgaagatgtc tctgaggata ccgcccaccc ccgtcgccgc
      481 cccctggtac ggttcgacga acgacggatg attgtgcgac tccatcttga agacggcgac
      541 gatcccgtcg ccgatatcga cgacgccggc gttctccccg ggtccctgga ccatcctggg
      601 gcccgtggtc gggagccgct tcagatggat tttcgaggac ttgtaggagc agtgctccga
      661 ccacatcacc gcgatgatgc cgagctccgt ccaggtcggc acgcgcccga ggagctgcac
      721 ggcccgctcg tactcctccg ccgtcatccc ctccttgatc gcgaggtcga ggtcgacctt
      781 ggggtccttc ggcgccgctg tcacgctcgc tctccgctcg attcggccgt catatccggt
      841 tccgtcgcgt ctgctgccag gtgtggagga tcgaggtgaa cagccgcagg ccgtcggcgt
      901 tgccgaggac ctcctcggcg caccggtccg gatgcggcat catccccagg acgttcccct
      961 tctcgttgca gatgccggcg atcgcctgca gcgacccgtt cgggttggct tccgccgtga
     1021 tctccccctt cgggtcgcag tagcggaaca ccacctgccg cttcttcgcc agctcctgca
     1081 ggacggcgtc cggcgcgtag tagcaccctt cgccgtgccc gatcgggatc ttcagcaccg
     1141 acccctgctt gatgcggttg gtgtacgcgg tgtcctgccg ctcgacccgc aggtacaggt
     1201 cgcggcagat gtactggagg ttggtgttgc gcaccagcgc ccccggcagc agccccgcct
     1261 cggcgagaat ctggaagccg ttgcagatgc cgaacaccat cccgccgcgg tcggcgtact
     1321 gcttgatcga tttcatgatg ggcgagaagc gcgccatggc gccggtgcgc aggtagtcgc
     1381 cgtgcgcgaa cccgccgggc aggacgatgc agtcgattcc cgcgagcgat tcgtccttgt
     1441 gccagatgaa ggtcgcctcg tggccgagga gatgcttgac cacgtgatag acgtcgtgct
     1501 cgcagttgct gccggggaag acgacgatcc cgaacttcac cgcagtgtca ctcgtccagg
     1561 aattcgaatc ggtagtcctc gatgaccgtg ttggcgagga gcttctggca catgtcgccg
     1621 agccgcttct ccgcctcctc gcgcggcagg ccgccgaggt tgacctccat gaacttgccg
     1681 atgcgcacgt ccgtcacctc gcggtagccg agctggtgca gcgacgtgca caccgccttc
     1741 ccctgcgggt cgagcactcc ccgcttgagg gtcacgtaca cccgcgcttt caagtggctt
     1801 cctccatccc gatgaggagg ctggatttta caccgtccgc gggccggcgt gcccgccggt
     1861 ggctaggcgg gcccgaagac gcgcttgaac agggtctcga cgtgccggag ctggtggccg
     1921 aggtcgaaac agcggtcgat ctcggcaacg ggcaggacgc gggtcacttc cgggttcccc
     1981 ttcagaagcg cccggaagtc gccgcctccc gagcgggcct gcatggcggc ggcctgcacc
     2041 cactcgtagg cctgatcccg cgtcaccccc cgctcggcca gcgccagcag cacgttcccc
     2101 gagtagatga ggccgcccga cgcctccagg ttccgctgca tcgccgcggc attcaccacc
     2161 atgccgttga cgatgcgcgt gaagcgggcg gtcatgtagt cgagc
//
LOCUS       DASRIQ010000236         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1918806, whole genome shotgun sequence.
ACCESSION   DASRIQ010000236 DASRIQ010000000
VERSION     DASRIQ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918806"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFD06_05840"
     CDS             complement(<1..194)
                     /locus_tag="VFD06_05840"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZN03091.1"
                     /translation="MRGSASDGSRDRLTVSALRLMVHIGVTEEERRARQRLEVDVELF
                     ADLEAAGRSGDLARTIDYRQV"
     gene            complement(175..1563)
                     /locus_tag="VFD06_05845"
     CDS             complement(175..1563)
                     /locus_tag="VFD06_05845"
                     /inference="COORDINATES: protein motif:HMM:NF040121.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HZN03092.1"
                     /translation="MALTNVLFVTGQLAKPSLEATLAALQPAGSAGPDGSVYSVAALR
                     IKVAALMTTDYIRHTLKPEPSARIVLPGLCQADTTALAAHFGTPVVKGPKDLRDLPEF
                     LGGEAQAYDPQGPHDLTLLAEINDVGGLDDAAVLKIAERSRDDGADVIDLGCSPERRH
                     PDLGRLVRRLIAEGHRVSIDTFDEREALAGDEAGAEMLLSLDGRNLHLAGRLHAVPVV
                     LPDPGKGIPSLLKNVAAAKERGAKRVIADPILEPIPFGLAASLVRFAEARAALPDTDL
                     LMGVGNLSELTEADSTGVNALLCGLMSELEVRYALTTEVAGWARGSVREIDCGRRMMH
                     HARTRKTLAKGFDHRLVTTRDPRVRRPREEELRALQSRVTDRNFRIDTDGEAIYIYNS
                     ERFVRTTDLRAVFDRLGVEDDASHAFYLGRELMKAEIARRLGKKYIQGEPLDWGYLSW
                     TEEEEPHARQRV"
     gene            complement(1554..2300)
                     /locus_tag="VFD06_05850"
     CDS             complement(1554..2300)
                     /locus_tag="VFD06_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN03093.1"
                     /translation="MPPSRTALVTGSTRGIGRAIAERLARDGFAVVVNHSSDSGAGRT
                     VDTIRAAGGVVHAVRADVSEPDECRRLVRAAQAAVGPLDVLVNNVGPFLERPLLETSD
                     DDWRMMVDGNLGSAFWCSREALHVMRERKGGAIVNIGALNAEVSPGMTHEAPAYFVAK
                     AGLMMLTRTLARTEGPHGIRVNAVSPGFIETESYADWNEAEQARWRSQIPLGRFGRPE
                     DVADAVAFLVSDRAAYVSGAILTVHGGLWL"
     gene            complement(2364..>2912)
                     /locus_tag="VFD06_05855"
     CDS             complement(2364..>2912)
                     /locus_tag="VFD06_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZN03094.1"
                     /translation="VVAALAAPPFRYSGNVAGPIDALVPDEARRTLARAATRIAARFE
                     LRGLNGIDFVLTERGPVVLEVNPRFTASMEILEERDEASYFDRHLQALDGTLPGAPAP
                     RPGWRAKGILYADRPVTAPDPESLVSFGVRDRPRRGEAFEPGRPLCTLVVRGADAADC
                     RRRLAERADQVRRLFFSVAGGA"
BASE COUNT          399 a         1034 c         1054 g          425 t
ORIGIN      
        1 acctggcggt agtcgatggt gcgcgccaga tcgccgctcc gcccggcggc ttcgaggtcg
       61 gcgaacagtt cgacgtccac ctccagccgc tgccgcgcgc ggcgctcctc ctcggtgacg
      121 ccgatgtgga ccatcagacg cagggcggac acggtcaggc gatcgcgcga gccgtcagac
      181 gcgctgccgc gcatgcggtt cctcctcctc ggtccacgac aggtagcccc agtcgagggg
      241 ctccccctgg atgtacttct tccccaggcg ccgggcgatc tccgccttca tcagctcgcg
      301 cccgagatag aacgcgtgcg aggcgtcgtc ctcgacgccg agacggtcga aaaccgcgcg
      361 caggtcggtc gtgcggacga agcgctcgct gttgtagatg tagatcgctt cgccgtcggt
      421 gtcgatacgg aaattccgat ccgtgacccg ggactgcaac gcacgcagct cctcctcccg
      481 cggccggcgg acgcgcgggt cccgggtcgt gacgagccgg tggtcgaacc ccttggccag
      541 cgtcttccgg gtccgggcat ggtgcatcat ccggcgcccg cagtcgatct cccgcacgct
      601 cccccgcgcc cacccggcca cctccgtcgt cagcgcgtag cgaacctcga gctcgctcat
      661 gagaccgcac aggagcgcgt tgacgccggt cgagtcggcc tccgtcagct cgctcaggtt
      721 cccgacaccc atgaggagat cggtgtccgg cagggcggcg cgcgcctcgg cgaagcgcac
      781 gagcgaggcc gccaggccga acgggatcgg ctcgaggatc gggtcggcga tgacccgctt
      841 ggccccccgc tccttcgcgg cggcgacgtt cttcagaagg gacgggatcc ccttccccgg
      901 atcgggcagc acgaccggca cggcgtggag gcgccccgcg agatggaggt ttctcccgtc
      961 gaggctcagg agcatctcgg cgccggcctc gtcgccggcg agcgcctccc gctcgtcgaa
     1021 ggtgtcgatg ctgacccggt gcccctcggc gatcaggcgg cgcacgagtc ggccgagatc
     1081 ggggtggcgc cgctccggcg agcagccgag atcgatgacg tcggccccgt cgtcccgtga
     1141 gcgctccgcg atcttcagga cggcggcgtc gtcgaggccg ccgacatcgt tgatctccgc
     1201 cagaagcgtc aggtcgtgcg ggccctgcgg gtcgtatgcc tgcgcctcgc cgccgaggaa
     1261 ctccggcagg tcgcgcaggt ctttgggccc tttcaccacc ggcgtgccga aatgcgcggc
     1321 gagcgccgtc gtgtcggcct ggcagagtcc gggaaggacg atgcgcgccg acggctccgg
     1381 cttgagggtg tggcggatgt agtcggtggt catcagcgcc gcgaccttga tgcgcagggc
     1441 ggcgacgctg tagacggatc cgtccgggcc ggcggatccg gccggctgaa gcgcggcgag
     1501 ggtcgcctcg agcgacggtt tcgccagctg gccggtgacg aagaggacgt tcgtcagagc
     1561 cacagccccc cgtggaccgt gaggatcgcc ccggagacgt aggccgcccg atccgacacg
     1621 aggaacgcga ccgcgtcggc gacgtcttcg ggccgcccga accggccgag cgggatctgc
     1681 gagcgccagc gcgcctgctc cgcttcgttc cagtcggcgt acgactcggt ctcgatgaat
     1741 cccggcgaga cggcgttgac gcggatcccg tgcggaccct cggtccgtgc cagggtgcgg
     1801 gtcagcatca tcagtcccgc cttggcgacg aaataggcgg gggcctcgtg cgtcatgccg
     1861 ggggagactt cggcgttgag cgcgccgatg ttgacgatcg cgccgccttt ccgctcccgc
     1921 atcacgtgga gcgcctcgcg gctgcaccag aaggcgctgc cgagattgcc gtccaccatc
     1981 atccgccagt cgtcgtcgct cgtctcgagg agcgggcgct ccaggaacgg cccgacgttg
     2041 ttgaccagga cgtcgagggg gccgacggcc gcctgcgccg cccgcaccag gcgccggcac
     2101 tcgtccggct cgctgacgtc ggcgcgcacc gcgtggacga ccccgccggc ggcccggatc
     2161 gtgtcgacgg tccggcccgc gccgctgtcg ctggagtggt tgaccaccac ggcgaagccg
     2221 tcgcgcgcga gtctttctgc gatggcgcgt ccgatgccgc gcgtgctccc cgtgaccagc
     2281 gccgtgcggg atggaggcat gtgcgtgtcg tccgttgaaa tgaggtttcg tctgagccta
     2341 tcacacggac cccggcgcgc cggtcaggcg ccgccggcga cggagaagaa gagcctccgc
     2401 acctggtcgg cgcgctccgc aagcctccgc cggcagtcgg cggcatcggc gccgcggacg
     2461 acgagggtgc agagcggccg gcccggctcg aacgcttcgc cgcggcgcgg ccggtcgcgg
     2521 acgccgaagg aaacgagcga ttcgggatcc ggtgccgtga cgggacggtc ggcgtagagg
     2581 atccctttcg cgcgccagcc gggccggggc gcgggagcgc cgggcagcgt tccgtcgagc
     2641 gcctggaggt gtcgatcgaa gtacgacgct tcgtcccgct cttcgaggat ctccatcgac
     2701 gcggtgaagc gcgggttcac ctccaggacg accgggccgc gctccgtcag gacgaagtcg
     2761 atcccgttga ggccgcgcag ctcgaagcgg gcggcgatcc gggtcgcggc gcgcgcgagc
     2821 gtgcggcgcg cctcgtccgg gaccagggcg tcgatcggcc ccgcgacgtt tccagagtaa
     2881 cgaaacggcg gcgccgccag cgccgcgacc ac
//
LOCUS       DASRIQ010000237        15165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2971996, whole genome shotgun sequence.
ACCESSION   DASRIQ010000237 DASRIQ010000000
VERSION     DASRIQ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 15165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15165
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2971996"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2118)
                     /locus_tag="VFD06_05860"
     CDS             complement(<1..2118)
                     /locus_tag="VFD06_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN03095.1"
                     /translation="MVGRTLGHYRIDGKLGSGGMGEVYLARDLKLDREVALKVLPTEM
                     AANPQRLQRFEREAKTVATLNHPNIVTIFSVEEADGVRFLTMERVVGQALDVRITPGG
                     MSFKPFMEVARPLADALAAAHEKGVVHRDLKPANIMVSDDGRVKVLDFGLAKLQEPDR
                     PEHVSELQTQGLTREGTIVGTVPYMSPEQLQGKAIDHRSDLFSLGVIYYQMLTGKRPF
                     HGGTSAETISAILRDTPHPVNEINVSLPNHLGRIVRRCLNKDPNERYQTARDLALDLK
                     ELKQEISDTERANVNSIAVLPFANMSPDPEQEYFCEGIAEELINGLGRIKNLRVASRT
                     SAFQFKGTKHDIREIGERLNVEKVVEGSVRKSGSQIRITAQVVNVADGYRLWSERYDR
                     KLEDIFAIQDEIAENIVRALEVTLSPKERRAIQNVATRDVEAYDFYLRGRKSFWEWRH
                     TGWTQARQMFERAIKLDPNYALAWAAIADCCSFTYMYADRSEKERLLADEASRKALQL
                     DPDLAEAHASRGMVLSLSDNNEQAAVHFEKAILLNPRLYEAYYFFARASVAQGQYAKA
                     ARLYEQAMEVRPEDYQVPLLLPQVYHSLHRAADEAAIARVGIEKAKRHLELNPDDPRA
                     LYLGGGAMAKLGVAEGNDAMVEEGLRMVDRTVTLTPDEVGVLYNVSCIYATLGRADEA
                     LTLLERAVRNGYNHLAWLETDSDF"
     gene            complement(2187..4883)
                     /locus_tag="VFD06_05865"
     CDS             complement(2187..4883)
                     /locus_tag="VFD06_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN03096.1"
                     /translation="MRRRDCAGFSLVEMLVASAIFAIVASVAFIFFSGAQKSYKSGGN
                     FAEQQQATRVAFDRMIADLRLAGFNTNPDGDASRVDEQVEGAWDTAVTIRGDFDFEDV
                     VLRSNPEASLPGTVYNVVSTGNDEVVTYVLAKPGPQGPATLTLRVDADRPRTRTVKTV
                     TIPNVALVQNDPPYTLYRVTLRDVAGAFLSSPQPASQFVYETVAENIRSMTFRYLPDS
                     GAVLGPDTPANPADDIGGDDAGILPRSRIRRISVSLVGMTPDEDLDYVDASDATATTH
                     FRKFDLKSDVNAENLGRAGVRDIDITPPPAPANLSLVPGHCKGMLVKWDTPSAASGVT
                     AYDIKYWPSGNPSAFTTGAVTYPHAEYGVVDFEGHGFLAGMTQGTTYCFQARARDIMG
                     NQSGWAPTTAPCAAVAEASNPAAPQNLAATGGTSLAPIDSQVTLTWDEVKNNANDVPN
                     DPDFINAHTLLRDGAGYKLYRDTTSTFTPDDATNLVAGPATLQPGVLTFTDTAVANCQ
                     PYYYKLKAADTCDVVGAASVAAVGQAQTNIVPSIPTGLSGQRSGAGAVTLTWNAVTAK
                     ADASAVHINLYRVYRYFAPRGTSPASLSTGAFTLRGTSSNPGYTDALSTQDEKAVEDG
                     QSLYYVVTAADLCGNESSKPMPVEVSCDFNGSLVVSPGDGDANGGSVRISMSVIGSDT
                     YVRGRVRIANPQVPGLFVYDETSSAYPFQFRDWNTTVSGLGKYQIYWEVQNAAGCVQT
                     LTTTFEATENLSCQITPTSPDLSPTNGKPSDQSKKLTWDIVNSSGRDLSIVTISASWT
                     SVLGPHKLVQIEYPTGTIVKSYGLLGPLVQLLADFTSLPLMLLDGVGSGCSGSSCVRM
                     SMVWDSQMMDSAGQGETITVTYGFKDPSGLGGTCSFSIKPTP"
     gene            complement(5051..6241)
                     /locus_tag="VFD06_05870"
     CDS             complement(5051..6241)
                     /locus_tag="VFD06_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZN03097.1"
                     /translation="MNLVTRLLVIAGAVLLGILLLWVAQSEIVLVTAEENGEFATSRY
                     VLAEEDFRHPRLVLLRERERLDAVLAGAPRQFEAAVRLRAWAHRQWDPGTSFHYPPWD
                     AVEILDLARRHDNRGFCAQYAIVFLQACQAMGIHARYVDLPGHFVVAIWSDDFDRWVL
                     MDPMHDLHYEKEGAPLRGRDLHRAYAAGDVAGLEQVDSAGRRQTVTLDDLAAFRLYSI
                     DTVANQLSQPVQVRINGAWRTLRHAKDHRVYPRVGRDQLEIESEFLAWRSGENAERFP
                     ERPESRDSDDFRYAMNQTIVLLANARVTQRILKVALVHQNSPTFARFQVRSEAGPDWV
                     PSPAETLKWLLHPGRNEIQARVETRDGWKGPASALRLYYKPALLPFLPQPGYVVRVMA
                     RRAG"
     gene            6341..7735
                     /locus_tag="VFD06_05875"
     CDS             6341..7735
                     /locus_tag="VFD06_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03098.1"
                     /translation="MRSRHFAGALCLAAALLALPAGARGAGQPRAGAEAAGQELEGPA
                     AARRADLVEFREKFFEQDRSYPAAARAEAEARLARLEKEAAGMEAAAFELEVARIVAL
                     ADNGHTVSFPGPRSRRYDRIPVRFVPFGERFFVLRAREPLSDLLGAELVAIDGRPMSE
                     LRAAARSLAGGVPSRRDRFASLLFESPEQLFALGFAAARDAATYRFAAAGGTNVERRI
                     AAEPADPARPRGNADRFLFPDPVEGEGSAWRTLLAPAQAPASLREPDVPFRWRPEPGI
                     EAIVIELRQNNDSGGRPIDRFLREATGAVRDAQPKNVVLDMRLNPGGDLGTTRDFAEG
                     LPALVPGRIFVLTSPWTFSAAISTVGYLKAKAPSRVTIVGEPVGDRLVFFAEGRPVTL
                     TNTGIVLLPATQRHDYRNGCRDFTDCHGAVVRHPIAVPTLDPEIAAPWTIEAYRSGRD
                     PGMEAVAAALARKS"
     gene            complement(7782..10145)
                     /locus_tag="VFD06_05880"
     CDS             complement(7782..10145)
                     /locus_tag="VFD06_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HZN03099.1"
                     /translation="MIDPLPIDAHVEAIAATVRARRALVLTAAPGAGKTTRVPPVLAA
                     DGPLILLQPRRVAARALARRIAEERGWTLGEEIGWQVRFERRFSPRTRLLVATEGILT
                     ARLLEDPLLEAFRTIVLDEFHERSLHADLALALARQAFLARHDLRLVVMSATLDTGPI
                     ARFLGDCPVLEVQGRPHPVAIEHRPGVPVATAVASVLGRHGGHLLCFLPGAPEIRRAQ
                     EEIAARAGGGGREAVTGPILLPLHGSLDAAEQDAALAPSKRRKVILATNIAETSLTVD
                     GVTDVIDTGLHKVLRNDPERGLDRLVTERIPLDAAMQRAGRAGRTGPGRVIRLWDPAE
                     ILRARREPEIARVDLAGALLDILAWGSAAHRFEWFEPPPRERIDAALDLLAALGAVRG
                     ESITALGRTLRRFPLHPRLARLLEAAGGSRTAAACCAVLSEEWTPPRGSGAPASTSSD
                     VLSRLDRLLEAPARVRNAASELQRLAERLLDRRREADDDATLRRAIFDAWPDRVARRR
                     APGSDRFVLASGHGASLGRESGVRDGEFVAALDVVGASTRSGASEALIRMASRVEPEW
                     IAPTRKETAHLFDAASGKVRASERWFYRDLLLRESPVPPDPGAAGEILAAECLARGPG
                     DEAAALLRRLRFADIEANPADLVRRACAGRTELPARLDLAAHLPAAAVRALARHAPET
                     LPLPSGRSARLDYREDGSVAAAVKLQELFGLGETPRLGPRREPVVFELLAPNGRPVQT
                     TRDLRSFWENTYPEVRRELRGRYPRHPWPEDPWTATPTHRATRRPRR"
     gene            complement(10142..11545)
                     /locus_tag="VFD06_05885"
     CDS             complement(10142..11545)
                     /locus_tag="VFD06_05885"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN03100.1"
                     /translation="MRISLSVALFVLLLPAGAQASQAASAAVDAAAVERVVGRLRAEE
                     QASQGAYQRLAWLCDRIGHRLSGSPGAAAAVGWALETMRRDGLKEVRAEKVMVPHWVR
                     GTAEITLLAPVARPLAGLALGMSVPTPPEGVEGEVIEVDSMEALESLGEKARGRVVLF
                     NKAIWRDPNGEGYGQVSPLRHAGPSQAARQGAVATLIRSLGTLPGRLTHTGTLSYADD
                     APRVPAAAIAQEDADLLHRLLASGEKVRVRLRLDCRTLPDAESANAVGEWRGRERPEE
                     IVVLGAHLDSWDVGTGAHDDGAGVVMALESIRLLKTLGLRPRRTIRAVLFMNEENGVR
                     GGKAYAADHAAELSRHVAALEADRGAGRPLGFSVGAGPGGADAVRRLAQGLAAIGATE
                     IVENGEGGVDISRLRPAGVPLLGLRSDMTSYFDWHHSEADTLDKIDRAALVDNVVAMA
                     AIAWALAEMDPPLPRQP"
     gene            11610..13733
                     /locus_tag="VFD06_05890"
     CDS             11610..13733
                     /locus_tag="VFD06_05890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN03101.1"
                     /translation="MASHRRLRTRPILHASFPLLLLAMGGALLSGPPAASGAGVAAAP
                     WKWTPETVADTTRLQDVILSPDGRRVVYAATRPRPEGAPFGAAYANLWLVDTAGGAAR
                     RLTSADAEDKSPAWSGDGRAIAFLSARGGEKAKTRLWVLSADGGEPWPLTSDKSDVES
                     FAWSREGRRIAYIAVDPKSEEREKEEKAGRDWKVVDHDDRPRRLFVVDVPAVGAVPPA
                     PRPVQALGERSAWQIAWAPDGRSLLATVTDTPRIDDAYMKKRIEILPIAGEGRGREVA
                     AVVGKVDEILWSRDGRTIVWRGGIDGSDPTTGSIFALAAGAAAGTKPANLTGNRPETV
                     RDLLWADGSAVAAITVQGTRTSIVAHDLKDPAVRRTLLDGGRLVFDDGSASNDGTRFA
                     FGGSSADGPEDVYTAKASIEPKARAGKTAAGPAGSFETARLTDLNPQLEGLPRGRQEV
                     IRYKASDGLEIEGVLIAPAGDPQRQSYPLVVIVHGGPEYEFHDGWVTRPSEPAQALAE
                     RGHFVFLPNYRGSAGRGVAFAKADHGDLGGREFQDVLDGIDWLIANRRIDRERVGMTG
                     GSYGGYFTSLAVTRYSDRFAAGAALFGISHWMSFLGQSDIPVENSAVHWNLWCYEHQE
                     TCAKASPVHHIARARTPTLILQGEEDKRVPKPQSDELYAALKWKGVPAEYVVFPRAKH
                     GFVEREHQIESTRLILDWFDRYLKP"
     gene            13756..14649
                     /locus_tag="VFD06_05895"
     CDS             13756..14649
                     /locus_tag="VFD06_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZN03102.1"
                     /translation="MPELPDITVYIEALEARVRGRRLEAIRLRSPFLVRSVDPPIAAA
                     EERLVERLVRLGKRIAFALEGDLFLVLHLMIAGRLKWGAKGMKVPGRLGLAAFDFDSG
                     TLVLTEAGSKKRASLHLVRGEAGLEPLRRGGLEVLDADASAFAAALRRENHTLKRALT
                     DPRLFSGIGNAYSDEILHRARLSPLALTRHLDDEEVERLRAATVATLEDWISRLRAET
                     GSGFPEKVTAFRPGMAVHGRFGKPCPDCGTAVQRIVSAENECNYCPRCQTGGRLLADR
                     ALSRLLKDDWPRTIDELEKEG"
     gene            14646..14945
                     /locus_tag="VFD06_05900"
     CDS             14646..14945
                     /locus_tag="VFD06_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03103.1"
                     /translation="MMRPTLSRVLLLAVAAVAVSAATAPAPPARRLALTFDDLPGADA
                     KAPLAELRDQNRRILEALRDAGAPARRAGSAEVDARRLRAAEPAVSAPRPEAARR"
     gene            complement(14950..>15165)
                     /locus_tag="VFD06_05905"
     CDS             complement(14950..>15165)
                     /locus_tag="VFD06_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03104.1"
                     /translation="EPQGEMEETLLGFTMIFLCVLPGAVGFALALNARSLSGPSGGTS
                     LTLPIVWNAVLLGLWFALMVVGVFQTP"
BASE COUNT         2113 a         5376 c         5486 g         2190 t
ORIGIN      
        1 gaagtcggag tcggtctcga gccaggcgag gtggttgtac ccgttgcgca cggcgcgctc
       61 gaggagggtg agcgcttcgt cggcgcgccc gagcgtcgcg tagatgcacg agacgttgta
      121 gagcacccct acctcgtccg gggtgagcgt gaccgtccgg tccaccatcc gcagcccttc
      181 ctccaccatc gcgtcgttcc cctcggcgac gccgagcttc gccatcgctc cgccgccgag
      241 gtacagggcg cgggggtcgt cggggttcag ctcgaggtgc cgcttcgcct tctcgatgcc
      301 gacccgggcg atcgccgcct cgtccgcggc ccggtgcagg ctgtggtaca cctggggcag
      361 gaggagaggc acctggtaat cctccgggcg tacctccatc gcctgctcgt acaggcgggc
      421 cgccttggcg tactgcccct gggcgacgct ggcgcgggcg aagaagtagt acgcctcgta
      481 gaggcgcggg ttgaggagga tggccttctc gaagtgcacc gccgcctgct cgttgttgtc
      541 ggagagggac agcaccatgc cgcgggaggc gtgcgcctcg gcgaggtccg gatcgagctg
      601 cagcgccttg cggctggcct cgtcggcgag caggcgctcc ttctcgctgc gatcggcgta
      661 catgtacgtg aacgagcagc agtcggcgat cgcagcccag gccagggcgt agttcgggtc
      721 gagcttgatg gcccgctcga acatctggcg tgcctgcgtc cacccggtgt gccgccactc
      781 ccagaacgac ttgcggccgc gcaggtagaa gtcgtacgcc tcgacgtcgc gggtggccac
      841 gttctggatg gcgcggcgct ccttcgggct cagggtcacc tcgagcgcgc gcacgatgtt
      901 ctcggcgatc tcgtcctgga tggcgaagat gtcctcgagc ttgcggtcgt agcgctcgga
      961 ccagaggcgg tagccgtcgg cgacgttgac gacctgcgcg gtgatgcgga tctgactccc
     1021 ggatttgcgg acgcttccct cgaccacctt ctcgacgttg aggcgctcgc cgatctcgcg
     1081 gatgtcgtgc ttggtccctt tgaactggaa ggcggaggtg cgcgaggcga cgcgcaggtt
     1141 cttgatgcgc ccgagaccgt tgatcagctc ctcggcgatc ccctcgcaga agtactcctg
     1201 ctccgggtcg gggctcatgt tggcgaacgg cagcacggcg atcgaattga cgttggcccg
     1261 ctcggtgtcg gagatctcct gcttcagctc cttgaggtcg agggcgagat cgcgggccgt
     1321 ctggtagcgc tcgttcgggt ccttgttgag gcagcgccgg acgatgcgcc cgagatggtt
     1381 cggcagcgac acgttgatct cgttcaccgg gtgcggcgtg tctcgcagga tcgcggagat
     1441 cgtctcggcc gaggtgccgc cgtggaacgg ccgcttgccg gtcagcatct ggtagtagat
     1501 gacgccgagc gagaagagat cggagcggtg atcgatcgcc ttcccctgca gctgctccgg
     1561 ggacatgtac ggcaccgtcc cgacgatcgt cccctcccgg gtcagaccct gcgtctgcag
     1621 ctcggagacg tgctccggcc ggtccggctc ctggagcttc gccagcccga agtcgagcac
     1681 cttcacgcgg ccgtcgtcgc tcaccatgat gttggccggc ttcaggtcgc ggtgaacgac
     1741 ccccttctcg tgggccgccg ccagcgcgtc ggccagcggc cgggcgacct ccatgaacgg
     1801 cttgaaggac atgccgccgg gggtgatccg gacgtcgagc gcctgcccga ccacgcgctc
     1861 catggtcagg aagcggacgc cgtccgcctc ctcgaccgag aagatcgtca cgatattcgg
     1921 gtggttgagg gtggcgaccg tcttcgcctc ccgctcgaag cgctgcagcc tctgcgggtt
     1981 ggccgccatc tccgtcggca gcaccttgag cgccacctcg cggtcgagct tcaggtcccg
     2041 ggcgaggtag acctccccca tcccgcccga tccgagcttc ccgtcgatcc tgtagtggcc
     2101 cagcgtcctg ccgaccatga gtgccgcaat ctacggcatc ggcaatccgc ggtcaatctc
     2161 gggtttcgcg gagacggcgc gaaccatcag ggggtgggct tgatgctgaa cgaacaggtc
     2221 ccgccgaggc cggaagggtc cttgaagccg taggtgacgg tgatcgtctc cccctgcccg
     2281 gcggaatcca tcatctggct gtcccagacc atcgacatgc ggacgcagga cgagccgctg
     2341 cacccggacc cgaccccgtc gaggagcatc agcggcaggg aggtgaagtc ggccagcagc
     2401 tggaccaggg gtccgaggag gccatagctc ttcacgatcg ttccggtcgg gtactcgatc
     2461 tgcaccagct tgtgcggccc gaggaccgag gtccaggacg ccgagatcgt gacgatcgac
     2521 aggtcgcgcc ccgagctgtt gacgatgtcc caggtcagct tcttgctctg gtcggacggc
     2581 ttgccgttgg taggggacaa gtccgggctg gtcggggtga tctgacagga caggttctcg
     2641 gtcgcctcga aggtcgtcgt cagcgtctgg acgcagccgg cggcgttctg cacctcccag
     2701 tagatctggt acttgcccag gcccgacacg gtcgtgttcc agtcccggaa ctggaagggg
     2761 tacgcgctgg acgtctcgtc gtagacgaag aggcccggga cctggggatt cgcgatgcgc
     2821 acccgccccc ggacgtaggt gtcggagccg atgacgctca tgctgatccg gacgctgccc
     2881 ccgttggcgt cgccgtcccc cggcgacacc acgaggctgc cgttgaagtc gcaggagacc
     2941 tcgaccggca ttggcttcga actctcgttg ccgcacaggt ccgccgcggt gacgacgtag
     3001 tagagcgact ggccgtcctc gaccgccttc tcgtcctgcg tgctcagcgc gtcggtgtag
     3061 ccggggttcg acgacgtccc acgcagcgtg aaggccccgg tcgagagcga agcgggggag
     3121 gtgccgcgcg gcgcgaagta gcggtacacg cggtagaggt tgatgtgcac cgccgaggcg
     3181 tcggccttgg ccgtcaccgc gttccaggtc aaggtcacgg cgccagcgcc gctgcgctgg
     3241 ccgctcagac cggtcgggat cgaggggacg atgttcgtct gcgcctgccc gaccgcggcg
     3301 acgctcgccg ccccgaccac gtcgcaggtg tccgccgcct tcagcttgta gtagtacggc
     3361 tggcagttcg cgaccgcggt gtcggtgaag gtcagcactc ccggttggag ggtcgccggg
     3421 ccggcgacga gattggtcgc gtcgtcgggg gtgaaggtcg aggtcgtgtc ccggtacagc
     3481 ttgtatccgg cgccgtcccg cagcagggtg tgggcgttga tgaagtccgg gtcgttcggg
     3541 acgtcgttgg cgttgttctt cacctcgtcc caggtcaggg tcacctggct gtcgatcggc
     3601 gccagagagg tcccgccggt cgcggccagg ttctgcggcg cggcggggtt cgaggcctcc
     3661 gcgaccgcgg cgcacggggc ggtcgtcgga gcccagccgc tctggttgcc catgatgtcc
     3721 cgggcgcgcg cctggaagca gtaggtggtc ccctgagtca tgccggccag gaagccgtgg
     3781 ccctcgaagt ccacgacgcc gtactcggcg tgcggatagg tgaccgcgcc ggtggtgaac
     3841 gcggacggat tcccgctcgg ccagtacttg atgtcgtagg cggtgacgcc ggaggcggcc
     3901 gacggcgtgt cccatttcac cagcatcccc ttgcagtgtc ccgggacgag cgacaggttc
     3961 gccggagccg gcgggggagt gatgtcgatg tcgcggacgc cggccctgcc caggttctcg
     4021 gcgttgacgt cgcttttcag atcgaacttg cggaaatgcg tcgtcgccgt ggcgtccgag
     4081 gcgtccacgt agtccaggtc ctcgtccggc gtcatgccga ccaggctgac gctgatgcgg
     4141 cggatccggc tgcggggcag gatcccggcg tcgtcgccgc cgatgtcgtc cgccgggttc
     4201 gccggcgtgt ccgggccgag caccgccccg gagtcgggga ggtagcggaa ggtcatgctg
     4261 cggatgttct cggcgaccgt ctcgtagacg aactggctgg ccggctgcgg cgaggagagg
     4321 aacgcgccgg cgacatctcg cagcgtcacc cggtacagcg tgtacggagg gtcgttctgc
     4381 accagcgcca cgttggggat ggtgaccgtc ttgaccgtcc tcgtgcgggg gcggtcggca
     4441 tcgacgcgga gcgtcagcgt cgcggggccc tgcgggcccg gcttcgccag gacatacgtg
     4501 acgacctcgt cgttgccggt cgagacgacg ttgtagaccg tcccggggag cgacgcctcg
     4561 gggttcgaac ggagcaccac gtcctcgaag tcgaagtcgc cgcggatggt gacggcggtg
     4621 tcccacgccc cctccacctg ctcgtcgacg cggctggcgt cgccgtccgg gttggtgttg
     4681 aacccggcga ggcggaggtc cgcgatcatc cggtcgaagg cgacccgggt cgcctgctgc
     4741 tgctcggcga agttcccgcc cgacttgtac gacttctgcg cgccactgaa gaagatgaag
     4801 gccaccgacg cgacgatcgc gaagatcgcc gacgccacca gcatctcgac caggctgaat
     4861 cccgcgcagt ctcggcgtcg catgccctcg aatagagcaa ccccggtgcc ggatttcctg
     4921 acgattgaga ggctgcaggc acaggagttc cggcccgttt cacggtttcg tcatcgggcg
     4981 cgtgtgcggg aacccatcac acccggtgtc ggattccggg cacgcgggga aagacggcgg
     5041 tgatggcgga tcagccggcg cgccgcgcca tgacccgcac cacgtatccc ggctgcggca
     5101 ggaacggcag gagcgccggc ttgtaataca ggcggagagc gctggccggt cctttccagc
     5161 cgtcgcgcgt ctccacgcgc gcctggatct cgttcctccc ggggtgcagc agccacttca
     5221 gcgtctccgc cggggagggg acccagtccg gccccgcctc ggagcggacc tggaagcgcg
     5281 cgaaggtcgg gctgttctgg tgcacgagcg ccaccttgag gatccgctgc gtgacgcgcg
     5341 cgttggccag caggacgatc gtctggttca tggcgtagcg gaagtcgtcc gagtcccggc
     5401 tctccggccg ctccgggaag cgctccgcgt tctcgccgga gcgccaggcg aggaactccg
     5461 actcgatctc gagctggtcg cggccgacgc gcgggtagac gcggtggtcc ttcgcgtgcc
     5521 gcagcgtcct ccacgcgccg ttgatgcgca cctggaccgg ctgcgagagc tggttcgcca
     5581 ccgtgtcgat ggaatagagc cggaacgccg cgagatcgtc gagcgtcacc gtctgccggc
     5641 ggccggccga gtcgacctgc tcgagcccgg ccacgtctcc cgccgcgtac gcgcggtgga
     5701 ggtcccggcc gcgcaggggg gccccttcct tttcatagtg gaggtcgtgc atcgggtcca
     5761 tcaggaccca gcggtcgaag tcgtcggacc agatggcgac gacgaagtgt ccggggagat
     5821 cgacgtagcg ggcgtggatc cccatcgcct ggcaggcctg caggaacacg atggcgtact
     5881 gggcgcagaa gccgcggttg tcgtgccggc gggccaggtc gaggatctcc accgcgtccc
     5941 acggcgggta gtggaaggag gtgcccggat cccactggcg gtgcgcccag gcgcgcagcc
     6001 ggaccgccgc ctcgaactga cgtggtgccc cggccagcac cgcgtcgagc cgctcccgct
     6061 cccgcagcag gaccaggcgg ggatggcgga aatcctcctc cgcgaggacg tagcgtgagg
     6121 tcgcgaactc gccgttctcc tcggccgtga ccaggacgat ctccgactgc gcgacccaga
     6181 gcagcaggat cccgagcaga accgcccccg cgatgacgag caggcgcgtc accaggttca
     6241 tttttgtgcc cgggcggccc gggcgcaagg aatccgagcc gggggtgcgg aacccgccgc
     6301 cccgggcggg cgtatctggc cgggacaaga ggagaacccg atgagatcac gccacttcgc
     6361 aggggcgctg tgcctggcgg cggccctgct ggccctgcca gccggggcgc gaggcgcggg
     6421 tcagccccgt gccggggcgg aggccgcggg tcaagagctt gaggggccgg cggcggcgcg
     6481 gcgcgccgac ctggtcgagt tccgcgagaa gttcttcgag caggatcgct cctatccggc
     6541 ggcggcccgg gcggaggcgg aggcccggct cgcccggctg gagaaggaag ccgccggcat
     6601 ggaggcggcc gccttcgagc tggaggtggc ccgcatcgtg gctctggccg acaacggcca
     6661 cacagtgtcg ttccccgggc cgcggtcgcg ccgctacgac cgtattcccg tgcgcttcgt
     6721 cccgttcggc gagcgcttct tcgtcctgcg cgccagagag cccctctccg acctgctcgg
     6781 cgccgagctc gtcgccatcg acgggcggcc catgtccgag ctgcgggccg ccgcgcgctc
     6841 gctcgccggc ggggtgccgt cgcgccggga ccgtttcgcc agcctcctgt tcgagagccc
     6901 ggagcaattg ttcgccctcg gcttcgccgc ggcgcgcgac gcggccacct atcgcttcgc
     6961 cgcggccggc gggacgaatg tcgagcggcg catcgccgcc gagccggccg atccggcgag
     7021 gccgcgcggc aacgccgacc gcttcctgtt ccccgacccg gtcgaagggg aggggagcgc
     7081 gtggcgaacg ctcctcgcgc cggcccaggc gccggcgtcg ctgcgggagc cggacgtccc
     7141 cttccgctgg cggccggagc cggggatcga agcgatcgtc atcgagctgc ggcagaacaa
     7201 cgacagcggc ggccggccga tcgatcgctt cctgcgcgag gcgacgggcg ccgttcggga
     7261 cgcgcagccg aagaacgtcg tcctcgacat gcgactcaat ccgggcggcg acctcggcac
     7321 cacgcgcgat ttcgcggagg gcctgccggc gctcgtcccg ggccggatct tcgtgctcac
     7381 cagcccatgg accttctcgg cggcgatctc gacggtcggg tatctcaaag cgaaggcgcc
     7441 gtcgcgcgtg accatcgtcg gtgagccggt cggcgatcgg ctggtcttct tcgccgaggg
     7501 gaggccggtg acgctgacga acaccgggat cgttctcctc ccggcgacgc agcggcacga
     7561 ctaccggaac ggctgccgcg atttcaccga ctgccacgga gcggtggtgc gccatccgat
     7621 cgccgtgccg acgctcgatc ccgagatcgc cgccccctgg accatcgagg cgtaccgctc
     7681 cggccgggat cccggcatgg aggcggtcgc cgcggcgctc gcccggaagt cctgacgccg
     7741 cgctccggca tgcgcactcc gcggccagcc gaccgagccg gtcagcgccg cggccgccgc
     7801 gtcgcgcggt gcgtcggcgt ggcggtccag ggatcctccg gccaggggtg tctcgggtag
     7861 cgcccacgca gctccctgcg gacttccgga taggtgttct cccagaagga gcgaaggtcg
     7921 cgcgtcgtct gcaccggccg cccgttcggc gccaggagct cgaacaccac cggctcgcga
     7981 cgcggcccga ggcgcggcgt ctcgccgagt ccgaacagct cctggagctt gaccgccgcc
     8041 gccacgctcc cgtcctcgcg gtagtcgagg cgggcggatc ggccgctcgg aagcgggagg
     8101 gtctcggggg cgtgccgcgc cagggcccgg acggccgccg ccggcaggtg ggcggcgagg
     8161 tcgaggcggg ccggcagctc ggtgcgcccg gcgcaggcgc gacggacgag atcggccggg
     8221 ttggcctcga tgtccgcgaa gcgcagccgg cgcaggagcg ccgccgcttc gtctccggga
     8281 ccgcgcgcca ggcactctgc cgccaggatc tccccggcgg cgcccggatc gggcgggacg
     8341 ggcgattcgc gcagcaggag atcgcggtag aaccagcgct ccgaggcgcg cacctttccg
     8401 gacgcggcgt cgaacaggtg cgccgtctct ttccgcgtcg gggcgatcca ctccggctcg
     8461 acgcggctgg ccatgcggat gagcgcctcg gaggcccccg accgggtgct cgccccgacc
     8521 acgtcgagcg cggcgacgaa ttcgccgtcg cggacgccgc tctcgcgccc gagcgaagcg
     8581 ccgtggcccg aggcgaggac gaagcgatcc gatcccgggg cacgacggcg cgccacgcgg
     8641 tcgggccagg cgtcgaagat cgcccggcgc agcgtggcgt cgtcgtccgc ttcccgccgc
     8701 cgatcgagca ggcgctccgc cagccgctgc agctccgagg cggcgtttcg cacccgcgcc
     8761 ggcgcctcga ggagccgatc gaggcggctc agcacgtcgg aggaggtcga tgccggcgcc
     8821 ccggaaccgc gcggcggggt ccactcctcc gacaggacgg cgcagcaggc cgcggcggtg
     8881 cgcgatcctc ccgccgcttc gagcaggcgc gccagccgcg ggtgcagcgg aaaccggcgg
     8941 agcgtccggc cgagcgcggt gatcgactct ccccgcaccg ctccgagcgc cgcgaggaga
     9001 tcgagcgccg cgtcgatccg ctctcgtggc ggtggctcga accactcgaa gcgatgcgcc
     9061 gcggatcccc acgccaggat gtcgaggagc gcccccgcca ggtcgacgcg ggcgatctcc
     9121 ggctcgcgcc gggcccggag gatctccgcg gggtcccaca gccgaatcac ccggcccggc
     9181 ccggtccgtc cggcgcgtcc ggcccgctgc atcgcggcgt cgagcggaat ccgctcggtg
     9241 acgagccgat cgaggccgcg ctccgggtcg ttcctcagga ccttgtgcag gccggtgtcg
     9301 atgacgtcgg tcaccccgtc caccgtgagc gacgtctcgg cgatgttggt cgcgaggatc
     9361 accttgcgcc gcttcgaggg ggcgagggcc gcatcctgct ccgccgcgtc gagcgagccg
     9421 tgcagaggga gcaggatcgg gccggtcacg gcctcccggc ccccgccgcc ggcgcgcgcc
     9481 gcgatctcct cctgcgcccg gcggatctcc ggagcgccgg ggaggaagca caggaggtgg
     9541 cccccgtggc gccccaggac cgacgcgacc gccgtcgcga cggggactcc ggggcggtgc
     9601 tcgatcgcca ccgggtgcgg ccgcccctgc acctcgagga ccgggcagtc gccgagaaac
     9661 ctcgcgatcg gcccggtgtc gagggtcgcc gacatgacga cgagccgcag atcgtggcgc
     9721 gccaggaacg cctgccgcgc cagcgccagg gcgagatcgg cgtgcaggct ccgctcgtgg
     9781 aactcgtcga ggacgatggt gcggaaggct tccaggagcg gatcttcgag gagccgcgcc
     9841 gtcaggatcc cctcggtcgc gaccaggaga cgcgtgcgcg gcgagaagcg ccgctcgaag
     9901 cggacctgcc agccgatctc ctcgccgagc gtccagcccc gctcctcggc gatgcgccgc
     9961 gccagggcgc gcgccgccac ccggcgcggc tgcaggagga tgagcgggcc gtcggcggcg
    10021 aggacgggag ggacgcgcgt cgtcttgccg gcccccggcg ccgccgtcag gacgagggcg
    10081 cgtcgcgcgc gcaccgtggc ggcgatcgcc tcgacgtgcg cgtcgatcgg cagcggatcg
    10141 atcacggctg gcgaggcagc gggggatcca tctcggcgag cgcccaggcg atcgcggcca
    10201 tggcgacgac gttgtccacg agcgccgccc ggtcgatctt gtcgagcgtg tcggcctcgc
    10261 tgtggtgcca gtcgaaatag gacgtcatgt cggagcgcag tccgaggagc ggcaccccgg
    10321 cgggacgcag gcgcgagatg tccacgcccc cctcgccgtt ctcgacgatc tccgtcgcgc
    10381 cgatcgcggc cagcccctgc gccagccgcc gcaccgcgtc cgcccctccc ggtccggcgc
    10441 cgacgctgaa gccgagcggc cggccggcgc cgcgatccgc ctcgagggcg gcgacgtgcc
    10501 gcgacagctc cgcggcgtga tcggcggcgt acgcctttcc gccgcgcacg ccgttctcct
    10561 cgttcatgaa gaggaccgcg cggatcgtgc gacgcggacg caggccgagc gtcttgagga
    10621 gccggatcga ctccagcgcc atcaccaccc cggccccgtc gtcgtgcgcg ccggtcccga
    10681 cgtcccacga gtcgaggtgc gcgcccagca cgacgatctc ctccggccgc tcccggccgc
    10741 gccattcgcc gacggcgttc gccgactccg cgtcgggaag cgtccggcag tcgaggcgca
    10801 gccggacccg caccttctca ccggacgcga ggaggcggtg gaggaggtcg gcgtcctcct
    10861 gggcgatcgc ggccgccggg acgcgcggcg cgtcgtcggc gtacgacagc gtgccggtgt
    10921 gagtcagccg ccccgggagc gtcccgagcg atcggatgag ggtggcaacc gccccctgcc
    10981 gcgccgcctg cgacggaccg gcatggcgca gcggcgacac ctggccgtag ccttcgccgt
    11041 tcgggtcacg ccagatcgcc ttgttgaaca ggacgacgcg gccgcgcgcc ttctccccga
    11101 gactctcgag agcctccatc gagtcgactt cgatcacctc cccctcgacc ccctcgggag
    11161 gggtgggcac gctcatgccg agcgccagcc ccgcgagcgg ccgcgcgacg ggggcgagga
    11221 gggtgatctc cgccgtgccg cggacccagt gcggcaccat caccttctcg gcccgcacct
    11281 ccttgagtcc gtcccggcgc atcgtctcga gggcccagcc gaccgcggcc gccgcccccg
    11341 gcgatccgga gaggcgatgg ccgatccggt cgcacagcca ggcgagccgc tggtacgccc
    11401 cctgcgaggc ctgttcctcc gcccgcagcc gcccgaccac gcgctcgacc gccgcggcgt
    11461 cgaccgccgc cgaagccgcc tgcgatgcct gcgcgccggc gggcaggagg agcacgaaga
    11521 gcgcgacgct caaggagatg cgcacggggg cggatgataa gatgaccgcc ccgcaagcgg
    11581 caacgactcg acgcgcaccg gaggtcacga tggcatcgca ccggcgtctc cgcacccgtc
    11641 ccatcctgca cgcttcattc ccgctcctgc tcctggccat gggaggcgcg ctcctctccg
    11701 gtcctccggc ggcgtccgga gccggggttg ccgccgcgcc gtggaagtgg acgccggaaa
    11761 cggtggccga cacgacccgc ctccaggacg tgatcctctc ccccgacggc cggcgcgtcg
    11821 tctacgccgc cacccggccc cgtcccgaag gggccccctt cggcgccgcg tacgcgaatc
    11881 tgtggctggt ggacacggcg ggaggcgcgg cgcgccggct cacgagcgcc gacgccgagg
    11941 acaagtcccc cgcctggtca ggggacggcc gggccatcgc cttcctgtcg gcgcgcggcg
    12001 gcgagaaagc gaagacccgc ctctgggtcc tgtcggcgga cggcggggag ccctggcctc
    12061 tgacgtcgga caagtcggac gtcgaatcgt tcgcctggtc gcgcgaaggg cgccgcatcg
    12121 cctacatcgc cgtcgatccg aagagcgagg agcgcgagaa agaggagaag gcgggccggg
    12181 actggaaggt cgtcgatcac gacgaccgac cgcgacggct cttcgtcgtg gacgtgccgg
    12241 cggtcggggc ggtgccgccg gcgccgcgtc cggtccaggc gctcggggag cgctcggcct
    12301 ggcagatcgc ctgggccccg gacgggcgtt cgctactggc caccgtgacc gacacgccgc
    12361 ggatcgacga cgcttacatg aagaagcgaa tcgagatcct gccgatcgcc ggcgaaggcc
    12421 gggggaggga ggtcgccgcg gtcgtcggca aggtggacga gatcctctgg tcgcgcgatg
    12481 gacggacgat cgtctggcgc ggcggcatcg acggatccga tccgacgacc ggcagcatct
    12541 tcgccctcgc cgccggtgcc gcggcgggga cgaagcccgc caacctgaca ggaaacaggc
    12601 ccgagacggt gcgcgatctt ctgtgggccg acggctcggc ggtcgccgcc atcacggtgc
    12661 aggggacccg cacctccatc gtcgcccatg acctgaagga tcccgcggtg cgccgcaccc
    12721 tcctcgacgg agggcggctg gtgttcgacg atggctcggc atccaacgac ggcacccgct
    12781 tcgctttcgg cggctcgagc gccgacggcc ccgaggacgt gtacacggcc aaagcgtcca
    12841 tcgagccgaa ggcgcgcgcc ggaaagaccg cggccgggcc ggcgggcagc ttcgagaccg
    12901 cgcgtctcac cgatctcaac ccgcagctcg aggggctgcc gcgcggtcgc caggaggtga
    12961 ttcgctacaa ggcctccgac ggcctcgaga tcgaaggggt gctgatcgcg cctgccgggg
    13021 atccgcagcg ccagtcgtac ccgctcgtcg tcatcgtgca cggcgggccg gagtacgagt
    13081 tccacgacgg ctgggtcacc cgcccgagcg agccggcgca ggcgctcgcc gagcgcggcc
    13141 acttcgtctt cctgccgaac taccgcggct ccgccgggcg cggcgttgcc ttcgcgaagg
    13201 cggaccacgg cgatctcgga gggcgcgaat tccaggacgt cctcgacggc atcgactggc
    13261 tcatcgcgaa ccggcgcatc gaccgggagc gggtcggtat gaccggcggc tcgtacggcg
    13321 gctacttcac gagcctcgcg gtcacgcgct acagcgaccg cttcgccgcc ggcgccgccc
    13381 tcttcggcat cagccactgg atgtcgttcc tcggtcagag cgacatcccg gtggagaaca
    13441 gcgccgtgca ctggaacctc tggtgctacg agcaccagga gacctgcgcc aaggccagcc
    13501 cggtgcacca catcgcccgg gcccgcaccc ccaccctcat cctgcagggg gaggaggaca
    13561 agcgcgtccc gaagccgcag agcgacgagc tgtacgccgc cctcaaatgg aagggggtcc
    13621 cggccgagta cgtcgtcttc ccgcgcgcga agcacggctt cgtcgagcgc gagcaccaga
    13681 tcgagtcgac gcgcctcatc ctcgactggt tcgaccggta cctgaagccc tgagcgggcg
    13741 aggcgccgga cggacgtgcc cgagctcccg gacatcaccg tctatatcga ggcgctcgag
    13801 gcgcgcgtgc gcggccgccg gctggaggcg atccgtctcc ggagcccctt cctcgtgcgc
    13861 agcgtcgatc cgccgatcgc cgcggccgag gagcgtctcg tcgagcgcct cgttcgcctc
    13921 gggaagcgga tcgccttcgc gctcgaaggg gatctgttcc tcgtcctgca cctcatgatc
    13981 gccgggcgcc tcaagtgggg ggcgaagggg atgaaggtcc cgggccggct ggggctcgcg
    14041 gcgttcgact tcgactcggg gacgctcgtt ctcacggagg ccggatcgaa gaagcgggcg
    14101 tcgctgcacc tggtgcgcgg cgaggcgggg ctcgagccgc tccgacgcgg cgggctcgag
    14161 gtcctcgacg ccgacgcttc tgccttcgcg gcggcgctgc gccgcgagaa ccacacgctg
    14221 aagcgggcgc tgaccgaccc gcgcctcttc tccggcatcg gcaacgccta ctcggacgag
    14281 atcctgcacc gggcgcgcct gtcgccgctg gccttgacca ggcatctcga cgacgaggag
    14341 gtcgagcgcc tgcgcgcggc gaccgtcgcg acgctcgaag actggatctc ccggctgcgc
    14401 gccgagaccg gctcggggtt ccctgagaaa gtgaccgcct tccgtcccgg catggcggtg
    14461 cacggccgct tcggcaagcc gtgtcccgac tgcggcacgg cggtgcagcg catcgtctcc
    14521 gccgagaatg agtgcaacta ctgcccgcgc tgccagaccg gcgggcgcct cctcgccgat
    14581 cgcgccctgt cgcgcctgct caaggacgac tggccccgca ccatcgacga gctggagaaa
    14641 gaggggtgat gcgacccacc ctctcgcggg tcctcctcct ggccgtcgcc gccgtggccg
    14701 tctccgcggc gaccgctccg gcgccgccgg cgaggcgcct ggcgctgacg ttcgacgacc
    14761 tgcccggggc tgacgcgaaa gcgccgctcg cggagctccg ggatcagaac cgccgcatcc
    14821 tcgaagcgct ccgggacgcg ggtgcgcctg cgcgacgagc cggatccgcc gaagtggatg
    14881 ctcgacgact acgcgcggcg gaacccgcgg tcagcgcgcc ccgcccggag gctgcgcggc
    14941 gctgagcgat cagggcgtct gaaagacccc gaccaccatc aacgcgaacc agagtccgag
    15001 caggacggcg ttccagacga tcggcagtgt gagcgaggtc ccgccggagg ggccggagag
    15061 gctccgggcg ttcagcgcca gggcgaaacc gaccgccccg ggcaggacgc agaggaagat
    15121 catggtgaag cccaggagcg tttcctccat ctcgccttga ggctc
//
LOCUS       DASRIQ010000238         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_353109, whole genome shotgun sequence.
ACCESSION   DASRIQ010000238 DASRIQ010000000
VERSION     DASRIQ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353109"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFD06_05910"
     CDS             <1..590
                     /locus_tag="VFD06_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN03105.1"
                     /translation="CDVLGFSLGGMIALQVAQDRPSIFRRMILAGTAPRGGEDIMHLE
                     KPALSRHFADPKLQGYAILTKIFFAPTESSQAAAQAFVARLMQSAQRREDRDPPSGPE
                     VAAAQMAAFREWEQFQGERFADLKGILHPTLVVNGVHDEMIPVSNSYRLAEHLPNAVL
                     LVYPDSGHGSLFQFHESFARQAAAFLSSESPFAPY"
     gene            complement(641..>3341)
                     /locus_tag="VFD06_05915"
     CDS             complement(641..>3341)
                     /locus_tag="VFD06_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HZN03106.1"
                     /translation="RFVGSQWEHLGADWNPPAFTENFLGFDRAGYLWAREIGRLLFLR
                     PGGTRFEVVEEGLDAIEFDVAGFLLDADGYVVTSPAWAPRSPAPKGGPTAYPPLKDHL
                     NGLIDREGGVWMSLGGLAHLWPEGALEDALNATRFEPGPLISGQASRELLLVASDGRS
                     QVENFNIHVYVLSKLLDREGNVWFGSSGGLHRFFYKRFFQLDLPDLTGPVAVVADGQG
                     GVWAGAQGTRLFHVPSGRPTWATRPGSWKVQVAYLGPAGDLWIGTSSDLWHQALSDRH
                     LVPATGERSATLAALQNHTGRAWERVDLPPQVAGKAGYLQALTQDRQGGLWVSLGRHG
                     LYRLADGVWTPDGGRDDLPKTGVVSEFTDAAGRVWFGFTRNQLAVLDGDRVRVFGPDD
                     GLRVGNVTAMAGRGPDLWIGGEFGLQRYSGGRLHAFVADDDLMLRGISGIVERANGDL
                     WLNGLTGIFHIDAAEIARAERSPAHRITGEHFGGRDGLPGFAAQLRPLASAFEGPDGR
                     LWFAVSSGVVWLDPESAQHPVSPLPITIQSVLVDDAPVHQDPPLRLPPHASTVQIAYA
                     AVSLSSPEAVRYRYRLSGIDKEWREVRVANPVTYRNLSPGTYRFTVGASNTNGAWSDR
                     LATVTFTLLPAFYQTGWFVALCAVAAAGALSLMFLFRLKQATRRLQSQLEARLGERER
                     IARELHDTLLQGVQGLLLKLQAVANRMPPEDPTRRSIEATLDDADRVVAEGRDRVRDL
                     RDGALPIRDLPAALRRVAEECPGGRIADLEVKVEGDARDLHPMVLEESYAIGREAVTN
                     ALTHSRGEHVAVEIGYAPAQFRLRVQDDGRGIDPAILETGGRDGHWGLQGMRERARRI
                     GAHLELRSGPGVGTEIELTVSAKTAYRSAVSRRSKPTVNPRSG"
BASE COUNT          547 a         1183 c         1092 g          519 t
ORIGIN      
        1 cctgcgacgt cctcggattc tccctgggcg ggatgatcgc gctgcaggtg gcgcaggatc
       61 gcccctcgat ctttcggcgg atgatcctcg cggggacggc tccgcgcggg ggcgaggaca
      121 tcatgcacct cgaaaagccc gcgctgtccc ggcacttcgc ggacccgaag ctccagggat
      181 acgccatcct gacgaagatc ttctttgcgc cgacggagtc gagccaggcg gccgcccagg
      241 ccttcgtcgc gagactgatg caatcggcgc aacgacggga ggatcgcgat cctccttccg
      301 gacccgaggt ggcggcggcg cagatggcgg cgttccgcga atgggagcag ttccaggggg
      361 agcgcttcgc ggatcttaag ggaatcctgc atccgacgct cgtcgtcaac ggcgtgcacg
      421 acgagatgat cccggtgtcg aactcgtatc gcctcgccga gcacctgccg aacgccgtcc
      481 tcctggtcta ccccgactcg ggacacggct ctctctttca gttccacgag tcgttcgcga
      541 gacaggcggc ggcgttcctg tcctccgaat cgccattcgc gccgtactga ctaagaggag
      601 atgaccgcat gtccgggaca ctcgcccgat ccggcgacgg tcaccctgat ctcggattca
      661 cggtgggctt cgatcttcga ctcacggcgg atcgataggc cgtcttcgcg gacacggtca
      721 gctcgatctc cgtgccaacg ccgggcccgc tgcgcagttc caggtgcgcc ccgatcctcc
      781 gggcccgctc gcgcatcccc tgcaggcccc agtggccgtc acggccaccc gtctcgagaa
      841 tcgccgggtc gatcccccgg ccgtcgtcct ggactctcag ccggaattgc gccggcgcat
      901 agccgatttc gaccgcgacg tgctcacctc gggaatgcgt cagcgcgttg gtcaccgctt
      961 cccggccgat cgcatacgac tcctccagca ccatcggatg caggtcccgt gcgtcaccct
     1021 cgaccttgac ctcgaggtcc gcgatccgcc ctccggggca ctcctccgcc acgcgccgca
     1081 gagcggccgg gaggtcgcgg attggaagcg cgccgtcgcg caggtcgcgg acacggtccc
     1141 tgccctccgc gacgacccga tcggcgtcgt cgagcgtcgc ctcgatggac cggcgcgtcg
     1201 ggtcttcggg cggcatccgg ttggccaccg cctgcagctt caagaggagc ccctggacgc
     1261 cttgaagcag cgtgtcgtgc agctcgcgag cgatccgctc ccgttcgccg agccgcgcct
     1321 ccagttgcga ttgcagcctc cgggtcgcct gcttcaaacg aaaaaggaac atcagggaga
     1381 gggcgccggc cgcagcgacc gcgcacaacg ccacgaacca ccccgtctgg tagaacgccg
     1441 gcagcagggt gaaggtgacg gtcgccaacc ggtccgacca cgccccgttc gtgttgctgg
     1501 cgccgacggt gaagcgatag gttcccggcg acaggttccg atacgtgacg gggttcgcga
     1561 cccggacctc gcgccattcc ttgtcgattc ccgacagcct gtagcgatac cgaaccgcct
     1621 cgggtgagga gaggctgacc gccgcgtagg caatctggac ggtcgaagcg tgcggcggga
     1681 gccgcagcgg cggatcctgg tggacgggtg catcatcgac caggaccgac tggatcgtga
     1741 tcggcaacgg ggacacagga tgctgcgcgc tttccgggtc cagccagacc acgccgctgg
     1801 acaccgcgaa ccacagacgc ccgtccgggc cctcgaaggc gctggcgagc gggcggagct
     1861 gcgcggcgaa ccccgggagt ccatctcgtc ctccgaaatg ctctccggtg atgcggtgcg
     1921 cggggctccg ctccgcccgc gcgatctccg cggcgtcgat gtggaagatg ccggtcaggc
     1981 cgttgagcca caagtccccg ttcgcccgct cgacgatgcc ggagatcccc cggagcatca
     2041 ggtcatcgtc cgcgacgaag gcgtgcaggc ggccgccgct gtagcgctgc aggccgaact
     2101 cgccgccgat ccagagatca ggcccgcgtc cggccatcgc ggtcacgttg ccgacgcgca
     2161 gaccgtcgtc aggtccgaag acgcgcacgc ggtcgccgtc cagcaccgca agctggttcc
     2221 tggtgaagcc gaaccagacg cgtccggcgg cgtccgtgaa ctccgagacc accccggtct
     2281 tcgggagatc gtcccggccg ccgtcgggag tccaaactcc atccgccagc cggtaaagcc
     2341 cgtggcgtcc cagcgagacc cacagtcccc cctgccgatc ctgcgtcaag gcctgcagat
     2401 aaccggcctt gccggcaacc tgcggcggaa gatcgactcg ctcccaggct cgcccggtgt
     2461 gattctggag tgcggcgagc gtcgcagacc gctcgccggt tgcggggacg aggtggcggt
     2521 cggaaagcgc ctggtgccag agatcgctgc tcgtgccgat ccagagatcg ccggcgggac
     2581 cgaggtacgc aacctggact ttccagctgc caggtctcgt cgcccaggtc ggcctcccgg
     2641 agggaacatg gaagagcctc gttccctgag cgccggccca gacgcctccc tgaccatcgg
     2701 cgaccaccgc cacgggtccc gtcaggtccg ggagatcaag ctggaagaac cgtttgtaga
     2761 agaagcggtg cagcccgccg gacgatccga accagacgtt cccttcccgg tccaggagct
     2821 tcgacaggac atacacatgg atattgaagt tttcgacctg gcttcgtccg tcgctcgcga
     2881 cgagcagcag ctcgcgcgag gcctggccgg aaatgagggg gcccggctcg aagcgtgtcg
     2941 cgttgagagc gtcttcgagc gctccctccg gccagagatg cgccaggccg cccagcgaca
     3001 tccagacacc cccctcccga tcgatcaggc cgttgaggtg gtccttcaga ggggggtagg
     3061 cggtcggccc ccccttcggc gcaggggacc gaggcgccca cgccggactg gtcacgacgt
     3121 acccgtcggc gtccagcagg aacccggcga catcgaattc gatggcgtcg aggccttcct
     3181 ccacgacttc aaagcgcgtg ccgccgggac gcaggaagag aagcctcccg atctctctcg
     3241 cccacaggta gccggcgcgg tcgaagccca ggaagttctc ggtgaatgcc ggcggattcc
     3301 agtcggctcc gagatgttcc cactgcgaac cgacgaacct c
//
LOCUS       DASRIQ010000239         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2727387, whole genome shotgun sequence.
ACCESSION   DASRIQ010000239 DASRIQ010000000
VERSION     DASRIQ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2727387"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFD06_05920"
     CDS             <1..545
                     /locus_tag="VFD06_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN03107.1"
                     /translation="RSRARAGDLVAVQGIGGLGHLAVQYARHMGFRVAAIARGRSKQE
                     LAMRLGAHHYVDSEATDPAKALQQLGGATVILATASSSKSQSGLLGGLRPGGTLLIVG
                     LDGGPIEIGSTDLVLAARSVDGSLTGSAQDAEDTLTFSVLQNIRSMNEILPLEEAAAG
                     YARMMSNSARFRIVLTMDKT"
     gene            complement(579..1643)
                     /locus_tag="VFD06_05925"
     CDS             complement(579..1643)
                     /locus_tag="VFD06_05925"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HZN03108.1"
                     /translation="MGATLALLTAVAIAPAAAADEPEKDFRQMYLFRFEFDNDNLLGS
                     DDAFTAGWSFQLHSPLDDTWGPAYAKWIGRVPGLGDDGRGGRIVRWAVGINQIILTPA
                     DISIEAPQPNDVPWAGILDVSLSWSAYDNQRMAALQLFVGCMGPCSGAESVQKFVHDD
                     LGFGETPEGWDNQLVNQALGNLNYEYRYKVMADEPDQYFNPSHFATDLAVGGEVGVGN
                     FATYVWGELEYRFGWGLPTGFTKTPDPPGFGMMLDPVYVDPVAPLPGRVPAWRNYFTL
                     MGRYAYFTYLAPSEGGETVAGDDHPKLRPYPGKYQALIGYHVARIPFAFHLTYYRYFN
                     EITDTGSSTDWVNLSFEYRF"
     gene            1777..2982
                     /locus_tag="VFD06_05930"
     CDS             1777..2982
                     /locus_tag="VFD06_05930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HZN03109.1"
                     /translation="MMMTGTTTKYLIVGGGMTGDAAVHGIREIDGTGSIRVIGSEPHP
                     SYNRPPLSKALWKGKPLESIWREPEERGVAWHRGRTAVLLDPRNRKVTDDQGAVHAYE
                     RLLLATGASPRRLPFGAGRVVYYRTLDDYRRLRDMTVKGRRFAVIGGGFIGSEIAAAL
                     AMNDREVVLLFPDDGICGRLFPPDLARSLNDYYREKGVEVMAGASVTGLDASGDRSVL
                     RVREGRTGRVTSIAVDGVVAGIGVQPNVDLAQAAGLEIDNGIRVDAGLRTKHPDIFAA
                     GDVASCHNPILDQWLRVEHEDNANTMGRLAGRAMAGEAVTYDHLSMFYSDLFDLGYEA
                     VGELDARLETVADWKEPFREGVVYYLRSGRVRGVLLWNVWEQVDAARQLIAEPGPIQA
                     RDLKGRLPA"
BASE COUNT          483 a          991 c         1076 g          465 t
ORIGIN      
        1 gccgttcgcg tgcgcgcgcc ggcgacctgg tggcggtgca agggatcggc gggctcggcc
       61 acctggccgt gcaatacgcc cggcacatgg ggttccgagt cgccgccatc gcgcgcgggc
      121 ggtccaagca ggagctcgcg atgcgcctcg gcgcgcacca ctacgtcgac agcgaggcga
      181 ccgatccggc gaaggcactt cagcagctcg gcggcgcgac ggtgatcctg gcgacggcgt
      241 caagcagcaa gtcgcagtcg gggctgctcg gcggattgcg ccccggcggc accctgctga
      301 tcgtcggcct cgacggcggg cccatcgaga tcggctcgac ggatctcgtg ctggccgcgc
      361 gctccgtcga cggctctctg acgggctccg cccaggacgc cgaggacacc ctgaccttca
      421 gcgtcctcca gaacatccgc tcgatgaacg agatcctgcc gctcgaggag gccgccgccg
      481 gctacgcgcg catgatgagc aacagcgcgc gcttccgcat cgtcttgacc atggacaaga
      541 cgtaagtccc cgtcgaggcc gcgggttcgg ccgcggacct agaagcggta ctcgaacgac
      601 agattgaccc agtcggtgga gctgccggtg tcggtgatct cgttgaagta gcggtagtag
      661 gtcaggtgga aggcgaacgg gatccgggcc acgtgatagc cgatgagcgc ctggtatttc
      721 cccgggtagg gccgcagctt cggatggtcg tcgccggcga ccgtctcccc cccctcggac
      781 ggcgccaggt aggtgaagta cgcatagcgt cccatcaagg tgaagtagtt gcgccaggcc
      841 ggcactcggc cggggagcgg cgccaccgga tcgacgtaga ccgggtcgag catcatgccg
      901 aagccggggg gatcgggcgt cttggtgaag ccggtcggca ggccccagcc gaagcggtat
      961 tcgagctcgc cccagacgta ggtggcgaag ttcccgacgc cgacctcacc tccgaccgcg
     1021 aggtcggtgg cgaagtgact gggattgaaa tactggtccg gctcgtcggc catgactttg
     1081 tagcggtact cgtagttcag gttgccgagc gcctggttca ccagctggtt gtcccagccc
     1141 tcaggggtct cgccgaagcc gagatcgtcg tggacgaact tctgcaccga ctccgccccc
     1201 gagcacggcc ccatgcagcc cacgaagagc tgcagcgccg ccatcctctg gttgtcgtac
     1261 gccgaccagg agaggctgac gtcgaggatg ccggcccagg ggacgtcgtt cggctgcggc
     1321 gcctcgatgc tgatgtcggc gggggtcagg atgatctggt tgatccccac cgcccagcgc
     1381 acgatgcgtc cgccgcggcc gtcgtccccc aggccgggca cgcggccgat ccacttcgcg
     1441 taggcgggac cccaggtgtc gtcgagcggc gaatgcagct ggaagctcca gccggccgtg
     1501 aacgcatcgt ccgatccgag gaggttgtcg ttgtcgaact cgaagcggaa gaggtacatc
     1561 tgccggaagt ccttctcggg ctcgtccgcg gccgccgccg gcgcgatcgc caccgccgtc
     1621 aggagggcca gcgttgcgcc cacgcagagg aaggccgccg gccggcgctg agatgcggag
     1681 cctgggagcg ggcggtgcat ggcggcggat tatcgaactc ttgtcgccac cgcgcaacag
     1741 cgtacaattt ctgcattcga accctgaggt gtcgcgatga tgatgacggg gacgaccacg
     1801 aagtatctga tcgtcggcgg cgggatgacc ggcgatgccg ccgtccacgg gatccgcgag
     1861 atcgatggca ccgggtcgat ccgcgtgatc ggcagcgagc cgcatccctc ttacaaccgg
     1921 ccgccgctgt cgaaagccct ctggaaaggc aagccgctcg agagcatctg gcgcgagccg
     1981 gaagagcgcg gcgtcgcctg gcaccggggg agaacggcgg tcctcctcga tccgcgcaat
     2041 cggaaggtga cggacgatca gggagcggtg catgcgtacg agcgcctgct cctcgcgacc
     2101 ggcgcctcgc ccagaaggct gccgttcggc gccgggcggg tcgtctatta ccgcaccctc
     2161 gacgactacc gccgcctgcg cgacatgact gtcaaggggc ggcgcttcgc ggtgatcggc
     2221 ggagggttca tcggctccga gatcgccgcg gcgctggcga tgaacgatcg cgaggtggtg
     2281 ctcctcttcc ccgacgacgg catctgtggc cgcctcttcc cgcccgatct cgcgcgatcg
     2341 ctgaacgact attaccgcga gaaaggggtc gaggtgatgg ccggcgcctc cgtgacggga
     2401 ctcgatgcgt cgggcgatcg atccgtcctc cgggtgcgcg aggggcgcac cggccgggtg
     2461 acctcgatcg cggtcgacgg tgtcgtcgcg gggatcggcg tgcagccgaa cgtcgacctg
     2521 gcgcaggccg ccggtctcga gatcgacaac ggcatccggg tggacgcggg gctgcgcacc
     2581 aagcaccccg acatcttcgc ggccggggac gtcgcctcct gccacaaccc gatcctcgac
     2641 cagtggctgc gcgtcgagca cgaggacaac gccaacacca tggggcgtct cgcgggacgc
     2701 gccatggcgg gggaggcggt gacctacgac cacctgtcga tgttctattc cgatctcttc
     2761 gatctgggct acgaggcggt cggcgagctc gacgcgcgtc tcgagacggt ggcggactgg
     2821 aaggagccgt tccgcgaggg tgtggtctat tacctgcgca gcggacgggt gcggggcgtc
     2881 ctcctgtgga acgtctggga gcaggtggac gccgcgcggc aactgatcgc cgagccggga
     2941 ccgatccagg cgagggatct caaggggcgg ctgcccgcct gagcacgcga gcgccaaatc
     3001 gacgtgcgac ctatt
//
LOCUS       DASRIQ010000240         7201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_354372, whole genome shotgun sequence.
ACCESSION   DASRIQ010000240 DASRIQ010000000
VERSION     DASRIQ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354372"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VFD06_05935"
     CDS             <1..1213
                     /locus_tag="VFD06_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03110.1"
                     /translation="RRFANVKDGIAFRLQARGGILYHHSNSMPKRLFPIRGFVWYVVD
                     VAYRVDTGTYDLVIREEGTATPVVARRDQPNASREPRSAVELFSFIGDPGTDESEVDY
                     FVDDVVLSADRPVNLAPFAAPGRRRLFVDAWLETVRQAGRAPGCPRATGPEDFGYSMD
                     DLRRLAREAGADLMTALRALPHGAGRRIAKPAALGALAAGLEPVFDWQRGCDALAAGK
                     AQEALAAFEEAARARPAGRVYHLSAIAALARLDRFSEAEARLDVLDASARGDPRLGLA
                     AAMIGLERPDLERLADGKDEGLAEEFFFVLLYRKTWTDAARFADRMAERARGLGRPDD
                     LWVEHAGDAAFLIGNDAAARTAYETALAARPGDRLLLTKLSDVAWRLGDTRLERQYRE
                     RVFGSLEKSDR"
     gene            1242..1700
                     /locus_tag="VFD06_05940"
     CDS             1242..1700
                     /locus_tag="VFD06_05940"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HZN03111.1"
                     /translation="MLASILSALHLLSLGIGLGAVFARGRALRALTSGDSSAVGRILF
                     ADNLWGLAAMLWIATGLTRLFGGFDKAPGFYYSNGFFWLKMGLFSLVFALEILPMVTF
                     IAWRGALRRGAAIDARRVPLLVRLNDAETALVLVIPFAAAAMARGVWLLA"
     gene            1754..2986
                     /locus_tag="VFD06_05945"
     CDS             1754..2986
                     /locus_tag="VFD06_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03112.1"
                     /translation="MTIERTAEGLEARLRPRGFGRWFTATFLLVWLCGWFAGECFAIW
                     MLVGGVRAFIAGTLLAQEQPDQPLAMAVLAGLFLLLWLSFWTLGGYAAGREFLRLVSS
                     EDRILVQHGTLVVRRRIGPFRSTRTVPRDEVAGIHAVARKNRVMAETKGGAVELTMLA
                     RGAEIESLVVALREDLGLGVGDSREALFLPDPWREVIDDEGRTVLVRDENQRRIRGRI
                     AWGLTALVAAASIPVFAGTRANPDGLPAACMLGSLALALGWGAWRISRTRLEWRIDPG
                     RLSLRRRSARGARELFEAQSLEFVGTTDSDDDRWYTLYAVAPGAPPTQVPPRLSDLRR
                     RRKIAREMDDPSMPRRLGTWIARRSGMRLDDRSPADGKPADLAELLGRLEASGRLGRW
                     LAKRLPRPDSGRGGGSAG"
     gene            complement(3008..3640)
                     /locus_tag="VFD06_05950"
     CDS             complement(3008..3640)
                     /locus_tag="VFD06_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZN03113.1"
                     /translation="MSRTLKLYDLTPSPNNIKVRCALAYKKLPYERIGVDPANREAVV
                     KASTQPLSPVLLHGETVVFDSYAIMRYLDANFPETPRLYSADRPTLKSIEAWELYART
                     DVGPGISMAFGQLRAETPDPDVMKRANDVVSKGASRVEEALSKSEWLLGETPTAADFT
                     VGTMLFLGAVPEAEVKRVPAWGFFRKHLKLERAPKTLDWVGRIMGYDRQP"
     gene            complement(3661..5172)
                     /locus_tag="VFD06_05955"
     CDS             complement(3661..5172)
                     /locus_tag="VFD06_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN03114.1"
                     /translation="MIPVLLTLALAAPVAATPRAAATAPASGLVERSVHVPFAGSATL
                     YVPPVRPSRAILFLSGDGGWNLGVVEMARRAARESGAVVAGLSLPAYLKAASREKVPC
                     WCPACDLEEIGKAVEKQAGFEEPSPPILLGYSSGATLVYAALAGGPPETFAGGMSLGF
                     CPDLEGVPPICRHAGWRPTYDAAKLRLDVPPVGLERPWVVLQGNADQVCAAAATDRFV
                     RAVEGATIARLDGVGHGYGNEKRWGAAFDAGLKTLMASSASPTPAGENPVAAGAATPE
                     SATVLEAALDRLDLPLVNRLTERPRAFLVFLSGDGGWSSLDRALANRLVRDHVDTIGI
                     SALQYFWKEKPAALVAADLRRILDLLRASGKPVLLGGYSFGAEVAPFLVNRSEPSFAG
                     LVLVAPGPYATWEVSALDWLRQKEKPSPDRVRPRIESLGEDLPTLCIYGAEDRESACS
                     GLASTSGRLVRALGGGHHFGGDYDVIADAVAGFVEKTIAAGVLTSASDPTPDR"
     gene            complement(5169..>7201)
                     /gene="mprF"
                     /locus_tag="VFD06_05960"
     CDS             complement(5169..>7201)
                     /gene="mprF"
                     /locus_tag="VFD06_05960"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HZN03115.1"
                     /translation="AAAAALLALVALALGGLERRGLVPAWLRRGAGSWRALLGVALAD
                     WIVAALVFVATLQACPERLPLGLALRGFFNGQAVGVLSLLPGGIGSADAFWLLRLPLP
                     QATLAAALVSYRIIYYVAPWLAACLVLLARGARSGARWLSPVPAVLGLLVAGAGTVML
                     VSTASPALEHRLHTLERWVPIGVVEASHLCGALSGLGLLFLARGVMRGYREAHRLTVV
                     LLLAAALAAGLKGLDYEEAGVLLALAAMVTSQSAQFRRSGRAPWVGWTALLLVGLAIG
                     IYTAVGFGSFPASAYEHAHWGRFGFGPHADQVARFLRGLALLALAGAGVLLLAGLRAA
                     ARFRAPDAAEIDRALASHARTGEGTTAMLVAAGDKSIFFQERDAFCSYRVVGSYVVVF
                     GDPTIAQGEERAFLDALIEHADALDRRLLFYQVSAAWLPSLHDRGFSFFKLGEEAILP
                     LADFSLAGGRWKSFRHAVRRVEEKEGGRFVVLTPDEVAARLPDLQRVSEEWLERRIGR
                     EKQFSVGAFGEGYMKRFPCAVVLDERDRVVAFANLLCGPNKGELSIDLMRHGRDAPDG
                     VMDYLFASLLEWGHKEGFATFNFGVAPLAAVGDLSGARLWERLAHLLFRHGEGLYHFQ
                     GLRSYKEKFQPRWVPRYMAYGESWEWPFAVVQVSVLIAGGWRAVLRPKKAAA"
BASE COUNT         1049 a         2614 c         2561 g          977 t
ORIGIN      
        1 gcggaggttc gcgaacgtga aggacggcat cgcgttccgg ctgcaggcgc gcggcggcat
       61 cctctaccac cactccaaca gcatgccgaa gcgcctgttc ccgattcggg gcttcgtctg
      121 gtacgtcgtg gacgtcgcgt atcgggtcga cacgggcacc tacgacctcg tcatccgcga
      181 ggaagggacc gcgaccccgg tcgtcgctcg gcgcgaccag ccgaacgcct cgcgcgagcc
      241 tcgctcggcg gtcgagctgt tctccttcat cggcgatccc gggaccgacg agtcggaggt
      301 ggactacttc gtggacgacg tcgtgctgtc ggccgaccgg ccggtgaacc tggccccctt
      361 cgccgcgccg gggcgccgcc gtctgttcgt ggacgcctgg ctcgagacgg tgcggcaggc
      421 cgggcgcgcg cccggctgcc cgcgcgcgac cgggccggag gatttcggct actccatgga
      481 cgacctccgc cgtctggcac gcgaggccgg cgccgatctg atgaccgccc tccgggcgct
      541 cccccatggc gcgggacgcc gcatcgcgaa gcccgccgcg ctcggcgcgc tggccgccgg
      601 actggagccg gttttcgact ggcagcgcgg gtgcgatgct ctcgccgccg gaaaggcgca
      661 agaggcgctc gcagcgttcg aggaggccgc gcgggcgaga ccggccggcc gcgtctacca
      721 tctgtccgcg atcgccgctc tcgcccggct cgatcgcttc tccgaggcgg aggcgcggct
      781 cgacgtgctc gacgcgtcgg cccgcggcga tccgcgcctc ggcctcgccg cggcgatgat
      841 cgggctcgaa cgccccgacc tcgagcgcct cgccgacggc aaggacgagg ggctggcgga
      901 ggagttcttc ttcgtcctcc tgtaccgcaa gacgtggacc gacgcggccc gtttcgcgga
      961 tcggatggcg gagcgggccc gcggcctcgg ccgccccgac gacctctggg tcgagcacgc
     1021 cggggatgcc gccttcctca ttggaaacga cgcggcggca cgcacggcgt acgagaccgc
     1081 gctcgccgcc cgccccggcg accgtcttct cctcacgaag ctgtccgatg tcgcctggcg
     1141 cctcggcgac acgcgcctcg agcgccagta ccgcgagcgc gtcttcggca gcctggaaaa
     1201 atccgaccga tagtccgtgt gcggtatcct gtgacttccg catgctggca tcgatccttt
     1261 cggcactgca cctgctgagc ctcggcatcg gactcggggc ggtgttcgcg cgcggccggg
     1321 ctctgcgtgc cctgacctcc ggggactctt ccgccgtcgg ccggatcctg ttcgccgaca
     1381 atctctgggg gctcgcggcg atgctgtgga tcgccaccgg cctgacgcgc ctcttcggcg
     1441 gattcgacaa ggctcccggg ttctactact cgaacggttt cttctggctg aagatgggcc
     1501 tcttcagcct ggtcttcgcc ctcgagatcc tgccgatggt gacgttcatc gcctggcgcg
     1561 gggcactgcg acgtggggcc gcgatcgacg cgcgacgggt cccgctgctc gtgcgcctca
     1621 atgacgcgga gacggcactc gtcctcgtca ttccgttcgc ggcggcggcg atggcccggg
     1681 gagtctggct gctggcctga ccgtcccgta atcgcccctt gacgccggga cgcacggagc
     1741 cgatcttcgc gtcatgacga tcgagcgcac cgcggagggg ctcgaagccc ggctgaggcc
     1801 gcgcgggttc ggacgctggt tcacggccac cttccttctc gtctggctct gcgggtggtt
     1861 cgcgggcgag tgcttcgcga tctggatgct cgtcggcggc gtgcgcgcgt tcatcgccgg
     1921 gacgctgctg gcccaggagc agccggacca accgctcgcc atggcggtcc tcgccggact
     1981 gttcctgctc ctgtggcttt cgttctggac gctgggaggc tatgcggccg gacgcgagtt
     2041 cctgcggctc gtctcgtccg aggaccggat cctcgtccag cacgggaccc tggtcgtgcg
     2101 gcggcggatc ggcccgttcc gctcgacgcg caccgtgccg cgggatgagg tcgccggcat
     2161 ccacgccgtc gcgcggaaga accgggtgat ggcggagacg aaagggggcg ctgtcgagct
     2221 gaccatgctg gctcgcggcg ccgaaatcga atcgctggtc gtagcacttc gggaggacct
     2281 cggcctgggg gtgggcgact cgcgggaggc cctcttcctg ccggatccgt ggcgcgaggt
     2341 catcgacgac gaagggcgca ccgtcctggt gcgcgacgag aaccagcggc gcatccgggg
     2401 ccgaatcgcg tgggggctga ccgccctggt cgcggccgcc tcgattccgg tcttcgccgg
     2461 cacgcgggcg aacccggacg gtctgccggc cgcctgcatg ctcgggtcgc tggcgctcgc
     2521 gctgggctgg ggggcgtggc ggatctcgcg cacccggctc gagtggcgca tcgaccccgg
     2581 acgactcagc ctcaggcgcc gatcggcgcg cggggcgcgg gagctgttcg aggcccagtc
     2641 gctcgaattc gtcgggacca ccgactcgga cgacgaccgc tggtacacgc tctatgccgt
     2701 cgcaccgggc gcccctccga cgcaggttcc gccgcgtctc tccgacctgc gccggcgccg
     2761 gaagatcgcc cgcgagatgg acgacccttc catgccgcgg cggctcggca cgtggatcgc
     2821 gcggcgctcc ggcatgcgcc tggacgatcg ctcgccggcc gatgggaagc cggcggacct
     2881 ggccgaactt ctcggccggc tcgaagcgag cgggcggctc ggccgctggc tcgcgaagcg
     2941 ccttccccgc cctgattcag gacgcggcgg aggttcggcg ggttaggtcg tcgtcccggc
     3001 cagtagccta cggctggcgg tcgtacccca tgatccggcc gacccagtcg agggtcttcg
     3061 gcgcccgctc gagcttcagg tgcttgcgga agaaccccca ggccgggacc cgcttgacct
     3121 ccgcctccgg caccgctccc aggaagagca tcgtcccgac ggtgaagtcg gccgctgtcg
     3181 gcgtctctcc gaggagccac tccgacttcg agagcgcctc ctcgacgcgc gacgccccct
     3241 tcgaaaccac gtcgttggcg cgcttcatca cgtccgggtc cggcgtctcc gcgcgcagct
     3301 ggccgaaggc catcgagatc cccggaccga cgtcggtgcg ggcgtaaagc tcccacgcct
     3361 cgatcgactt cagcgtggga cggtcggcgc tgtagagacg cggcgtctcg ggaaagttgg
     3421 cgtcgaggta acgcatgatg gcgtacgaat cgaagacgac cgtctcgccg tgcagcagca
     3481 ccggggacag cggctgcgtg gacgccttga ccaccgcctc gcggttggcg ggatcgaccc
     3541 cgatccgctc gtagggcagc ttcttgtacg ccagggcgca gcgtaccttg atgttgttcg
     3601 gagacggggt gaggtcgtag agcttgagcg tccgggacat ggtttctcct tgaagcgcgg
     3661 tcagcgatca ggggtgggat cggaggcgga tgttagcacg cctgccgcga tcgtcttctc
     3721 gacgaagccg gcgacggcgt cggcgatgac gtcgtagtcg ccgccgaagt gatgtccgcc
     3781 gccgagcgcc cggacgagcc gccccgaggt cgaagcaagg cccgagcagg ccgattcccg
     3841 atcctcggcg ccatagatgc acaaggtcgg cagatcctcc ccgagcgact cgatccgcgg
     3901 ccgcacgcgg tccgggctcg gcttctcctt ctgtctcagc cagtccagcg cgctcacttc
     3961 ccaggtcgcg tacgggcccg gagcgacgag gaccagcccg gcgaacgacg gctcggaacg
     4021 gttcacgagg aacggcgcca cctcggcgcc gaacgaatac ccccccagca ggaccggctt
     4081 ccccgaggcg cgcagcaggt ccaggatgcg gcgcagatcg gccgccacga gcgcggccgg
     4141 cttctccttc cagaagtact gcagcgcgct gatgccgatc gtgtcgacgt ggtcccggac
     4201 gaggcggttg gcgagggcgc ggtcgaggct cgaccagccg ccgtcccccg acaggaagac
     4261 aaggaaggcg cgcggccgct cggtcagacg gttgaccaga ggcagatcga gccggtcgag
     4321 cgccgcctcc agaacggtgg cgctttcggg cgtcgcggcg ccggctgcga ccggattctc
     4381 gcccgcgggc gtcggtgagg ccgacgaagc catgagcgtc ttgagccccg cgtcgaaggc
     4441 ggctccccag cgtttctcgt tgccgtagcc gtggccgacg ccgtcgaggc gcgcgatcgt
     4501 cgccccctcc accgcgcgca cgaaccggtc ggtcgcggcg gcggcgcaca cctggtcggc
     4561 attcccctgc agcacgaccc acggccgctc gagaccgacc ggcgggacat cgagccgcag
     4621 cttcgcggcg tcgtacgtgg ggcgccagcc ggcgtgacgg cagatcggcg gcacgccctc
     4681 gagatcgggg cagaaaccga gactcatccc gccggcgaag gtctccggcg gccccccggc
     4741 gagcgccgca tacacgaggg tcgcgccgct cgagtagccg agaaggatgg gggggctcgg
     4801 ctcctcgaac ccggcctgct tctcgaccgc cttgccaatc tcctcgagat cgcaggcggg
     4861 gcaccagcac gggaccttct cgcgggatgc cgctttcaga tacgccggga gggagagacc
     4921 ggcgacgaca gcgccgctct cccgcgcagc ccgtcgcgcc atctcgacga cgccgaggtt
     4981 ccagccgccg tcgccgctca ggaagaggat ggcgcggctc ggccggaccg ggggcacgta
     5041 cagggtggcg ctcccggcga acgggacgtg cacggagcgc tccacgagcc cggaggccgg
     5101 cgccgtggcg gccgcgcgcg gcgtcgcggc caccggcgcg gcgagcgcca gggtgagaag
     5161 gacggggatc acgccgcggc cttcttcgga cgcagcaccg cccgccagcc tcccgcgatc
     5221 agcaccgaca cctgcacgac cgcgaacggc cactcccacg actcgccata ggccatatac
     5281 cggggcaccc agcggggctg gaacttctcc ttgtacgacc tcaggccctg gaaatggtac
     5341 agcccctcgc cgtgccggaa caggaggtgc gccagccgct cccacaggcg cgccccgctc
     5401 aggtcgccca ccgcggcgag cggcgccacg ccgaagttga aggtggcgaa cccttccttg
     5461 tgtccccact cgagcaggct ggcgaagagg tagtccatga ctccgtcggg agcatcgcgg
     5521 ccgtggcgca tgaggtcgat cgacagctct cccttgttcg ggccgcacag gaggttcgcg
     5581 aaagccacca cacggtcccg ctcgtcgagg acgacggcgc acgggaagcg cttcatgtac
     5641 ccctcgccga aggcgccgac ggagaactgc ttctcccgcc cgatgcgccg ctcgagccac
     5701 tcctccgaca cgcgctggag atccggcagg cgcgccgcga cttcgtcagg cgtcagcacc
     5761 acgaagcggc ccccctcttt ctcctcgacg cggcgcaccg cgtgccggaa ggacttccag
     5821 cggccgcccg cgagggagaa gtcggcgagc ggcaggatcg cctcctcccc cagcttgaag
     5881 aaagagaagc cgcggtcgtg caggctcggg agccatgcgg ccgagacctg gtagaagagg
     5941 aggcgccggt cgagcgcgtc ggcgtgctcg atgagcgcgt cgaggaaggc gcgctcctct
     6001 ccttgcgcga tggtcgggtc gccgaagacg acgacatacg agccgacgac ccgataggag
     6061 cagaaggcgt cgcgctcctg gaagaagatc gacttgtccc ccgccgccac gagcatcgcg
     6121 gtcgtcccct cccccgtgcg ggcgtgcgac gcgagggccc gatcgatctc cgccgcgtcc
     6181 ggcgcccgga acctcgcggc ggcccgcagc ccggcgagga gcagcacacc ggcccccgcc
     6241 agcgccagga gcgccaggcc gcgcaggaag cgggccacct ggtcggcgtg cggaccgaac
     6301 ccgaagcgtc cccagtgggc gtgctcgtag gccgacgccg ggaacgaccc gaacccgacg
     6361 gcggtgtaga tgccgatcgc gaggccgacg agcagaaggg cggtccaccc gacccacggc
     6421 gcccggccgg agcggcgaaa ctgcgccgac tgcgaggtga ccatggccgc gagcgcgagc
     6481 aggacccccg cctcctcgta gtcgagcccc ttgagccccg cggcgagcgc cgccgcgagc
     6541 aggaggacga ccgtgagccg gtgcgcctcg cgatatccgc gcatcacgcc gcgcgcgagg
     6601 aagagaagcc cgagccccga cagcgcgccg cacaggtgcg acgcctcgac gacgccgatc
     6661 gggacccagc gttcgagcgt gtgaaggcgg tgctcgagcg ccggcgaggc ggtgctcacg
     6721 agcatcaccg tcccggcgcc ggcgacgaga agaccgagga ccgccggcac cgggctcagc
     6781 cagcgggcgc cgctcctggc gccgcgcgcc agcagaacca ggcaggcggc gagccagggg
     6841 gcaacgtaat agatgatgcg gtaggagacg agcgccgcgg cgagggtcgc ctgcggcagc
     6901 ggcaggcgca gcagccagaa cgcatcggcg ctgccgatcc cccccggcaa gagcgacagc
     6961 acgccgaccg cctgcccgtt gaagaatccc cgaagcgcca ggccgagcgg cagccgctcc
     7021 gggcacgcct ggagggtcgc gacgaacacc agcgccgcga cgatccagtc ggccagcgcg
     7081 acgccgagaa gcgcgcgcca cgaacccgca ccgcgccgga gccacgccgg gacgagcccg
     7141 cgccgctcga gcccccccag cgccagcgcg acgagcgcca ggagcgcggc cgccgccgcg
     7201 a
//
LOCUS       DASRIQ010000241         5827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2728347, whole genome shotgun sequence.
ACCESSION   DASRIQ010000241 DASRIQ010000000
VERSION     DASRIQ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2728347"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..978)
                     /locus_tag="VFD06_05965"
     CDS             complement(22..978)
                     /locus_tag="VFD06_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03116.1"
                     /translation="MRTGRRETTRILLSWALVTGAGCASARASVALEATNRMQDQWTG
                     AWVVTSTETFSDCAGFYTDNEVSGDDSVASRGRTRIDAGTPARVDSVDVRPERLSLRL
                     TLSVNQMFERPHREFTLRALARCQAEVRVMVPPGIVEGGDVTGLESLLDRVVERHSDE
                     RSALLASNFLPVDTPEYAAERQEAIAEHREWRQEQTAEALKARLEQWTAQTTRLSKGI
                     SQDPDYLAGFGKGVEAGRNSVPSACEEVTRFEPALQPARMNAGAAFAAKGDRQKNWTR
                     GYEDGMRLAQGLEVLRLMPGCMPPAPAPRPVKADPAASAVRR"
     gene            1301..4606
                     /locus_tag="VFD06_05970"
     CDS             1301..4606
                     /locus_tag="VFD06_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZN03117.1"
                     /translation="MTTFVSAALVAALFAVPSGAAASKDSRSKEKAKGPETRFKPETF
                     SGLELRGIGPAVTSGRIIDIAVQPNDRATWYAAVASAGVFKTTNAGTTWSSVFEGQGS
                     YSIGCVTLDPKNPLVVWVGSGENNSQRSVGYGDGVYKSTDGGASWENVGLKASEHIGK
                     IAIDPRDSNVVYVAAQGPLWGPGGDRGLYKTTDGGKTWKQVLKISENTGVSEVVLDPA
                     HPDVLYAAAYQRRRHVWTLVNGGPESAIHKSVDGGATWKKLENGLPKEEMGRIGLAIS
                     PVQTDTVFAVIEAANKAGGVYRTRDAGGTWEKAGDYVPGGPQYYMELFPDPKNPNRLY
                     SMDVFMQVSEDAGTTWKKAGEKSKHVDNHALWIDPDDPDHLLNGCDGGVYESWDRGAT
                     WDFKANLPVTQFYKLAIDNAEPFYNVYGGTQDNYTLGGPSRTFSTHGITNADWFVAQE
                     GDGFQAQVDPKDPNIVYAEAQHGAIIRLDRKTGERIDIQPQPDKGEDGSRWNWDSPLI
                     ISPHSNTRLYFASQRVYRSDDRGDSWRAVSPDLTRQIDRNRLKVMGRVQSVDSIAKNA
                     STSFYGNIVALAESPKREGLLYVGTDDGLVQVSADSGKTWRKAESFPGVPDRTYVSRL
                     TASIHSAGRVFAAFNNHKMADFKPYLLKSDDEGKSWTSIAGDLPERGSVWAIAEDHVD
                     PALLFAGTEFGLFFTVDGGGHWVQLKGKFPVIPVRDIAIQKRENDLVVATFGRGFYIL
                     DDYTPLRGLRASTLEQDAVLFPIKQAPLYQPTNWLGLKDKAFQGESFFNAPNPPFGAV
                     FTYYLKDELLTQKKVRQKAEKETIKNAGDVFYPTWEALKAEDREEDPVILMTVSDASG
                     QVVRQITGPVTAGFHRVAWDLRYPPSQPALLKVPDPEPFDDPRQGPFAPPGEYTVRLA
                     KRVNGVTTPLAAPMVVEAEGIGASSLPPADRRAVAEYSRKVARLQRAVLGAEEAAGEA
                     QNRILLVKRAIQDTPGADPKLLDEAWAIEARLKDLMVALTGDTTRSRRNEPTSPSISG
                     RVNQVVGGFWSTTSAPTATHKRNYDIAAAEFTPLLASLRSLIDDDLRKIETAAETAGA
                     PWTPGRVPVWSAE"
     gene            complement(4595..>5827)
                     /locus_tag="VFD06_05975"
     CDS             complement(4595..>5827)
                     /locus_tag="VFD06_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZN03118.1"
                     /translation="RHNDNPRFWGYVNPPGLAAGALGDWLASGLNQNLTAFRSAPGAA
                     LVEMQVVGWIREMVGLPRSAGGLLTSGGSVANLAGLAAARDAAVPFDLARRGLRGEPG
                     PPLVMYASEEVHHAVLKAASILGIGRDFVRLVPVDRRLRIDVAQLREAVQRDRRARLL
                     PFAVVASAGTVTTGTVDPLRHVWRLCRRERLWMHVDACYGGFAILTPSGRPILRALSL
                     ADSIALDPHKWLYLPVDAGCVLYRRPEAARRAFRQEADYTRMFGAGRLEQTAFWDYGP
                     ELSRRFRALKIWLALKHHGAEAFSASIEEDLQLARALADKVRATRRLELLSRGDLGIV
                     CFRYVPPGKRLPEVDLTRLNEAVLLAVQRRGRAFVSNARVRGRFALRACLVNYRTTEA
                     DLDVLLGEVTEAGVSLLR"
BASE COUNT          944 a         2042 c         2024 g          817 t
ORIGIN      
        1 ggccggtgct gggcgggcgg ttcatcggcg caccgccgag gcggccgggt cggccttgac
       61 cgggcgcggc gccggcgccg gtggcatgca gccgggcatc agccggagga cttcgagccc
      121 ctgcgccagc cgcatcccgt cctcgtagcc gcgcgtccag ttcttctgcc ggtccccctt
      181 cgccgcgaag gcggcgcccg cgttcatccg cgccggctgc agcgccggct cgaagcgggt
      241 cacctcttcg cacgcggacg gcacgctgtt gcgacccgct tccactccct ttccgaagcc
      301 ggcgagatag tccgggtcct ggctgatccc cttcgacagg cgggtcgtct gggcggtcca
      361 ctgttcgaga cgcgccttga gcgcctcggc cgtctgctcc tggcgccact cgcggtgctc
      421 ggcgatcgcc tcctggcgct ccgccgcgta ctccggcgtg tccaccggca ggaagttcga
      481 ggcgaggagc gccgatcgct cgtcggagtg ccgctcgacg acgcgatcga ggagcgattc
      541 gagcccggtc acatccccgc cttcgacgat gccgggcgga accatcaccc gcacctcggc
      601 ctggcaacgc gccagcgccc gcagggtgaa ctcgcggtgc ggccgctcga acatctggtt
      661 caccgacagg gtgagcctca ggctcaggcg ctcggggcgc acgtccacgc tgtcgacgcg
      721 agccggcgtc ccggcgtcga tgcgggttcg gccgcggctc gcgaccgagt cgtcgccgga
      781 gacctcgttg tcggtgtaga agccggcgca atcggagaag gtctcggtgg aggtgacgac
      841 ccaggctccg gtccactggt cctgcatccg gttcgtcgcc tccagcgcca ccgaggcgcg
      901 ggcggaggcg caaccggccc ccgtgaccag cgcccatgac aacaggatcc tcgtcgtctc
      961 ccgccgtccc gtccgcatct cggcatcctc tcccggctcc cgccggctcc gaatcccgcc
     1021 tccgtcacga ctttccagcg aaccctgtcc ctgaacctag ggaggggcgt cgtgatcgtc
     1081 aacggccgtt ttccggatcg ccgcgccggt tacggagttg cgcggaagcg atttttccgt
     1141 tacaacgcgc aggtccccgg agaagctgag gcccccggac ggcggcggtt acaacgctgc
     1201 ccggccactt acggaacggg cccggctgac agaaccgtcc tccggaaggc gcgttcaaag
     1261 tcgcgacagg gagaaagcca tggaaggccg aattcgccgg atgaccacat tcgtttccgc
     1321 cgccctcgtg gcggccctct tcgccgtccc ctcgggcgcc gccgcctcca aagactcgcg
     1381 gagtaaggag aaagccaagg gacccgagac ccgcttcaag ccggagacct tctccggcct
     1441 cgagctgcgc ggcatcggtc cggcggtcac ctccgggcgg atcatcgaca tcgcggtgca
     1501 gccgaacgat cgcgccacct ggtacgccgc ggtcgcctcc gccggcgtct tcaagacgac
     1561 caacgccggc acgacctggt cctccgtttt cgaaggacag gggtcctact cgatcggctg
     1621 cgtcacgctc gacccgaaga acccgctggt cgtctgggtc ggctcgggcg agaacaacag
     1681 ccagcggagc gtcggatacg gcgatggggt ctacaagtcc accgacggcg gcgcgtcgtg
     1741 ggagaacgtc ggcctcaagg cgagcgagca catcgggaag atcgccatcg acccgcgcga
     1801 ctcgaacgtc gtgtacgtgg cggcgcaggg gccgctgtgg ggcccggggg gggatcgtgg
     1861 cctctacaag acgaccgacg gtggcaaaac gtggaagcag gtgctgaaga tcagcgagaa
     1921 caccggcgtc agcgaggtgg tcctcgatcc ggcgcacccc gacgtcctgt acgccgccgc
     1981 ctaccagcgg cgccggcacg tctggacgct cgtcaacggc ggccccgagt cggccatcca
     2041 caagtcggtg gacggcggcg ccacgtggaa gaagctcgag aacggcctcc ccaaggagga
     2101 gatgggacgg atcggtctgg cgatctcccc ggtgcagacc gacacggtct tcgcggtgat
     2161 cgaggcggcc aacaaggcgg ggggcgtgta ccgcacgcgc gacgccggcg ggacctggga
     2221 gaaggccggc gactacgtcc cgggcgggcc gcagtactac atggagctgt tccccgaccc
     2281 gaagaacccg aaccggctct actcgatgga cgtcttcatg caggtcagcg aggatgccgg
     2341 gaccacctgg aagaaggcgg gtgagaagag caagcacgtg gacaaccacg ccctgtggat
     2401 cgacccggac gaccccgacc acctgctcaa cggctgcgac ggcggcgtgt acgagtcgtg
     2461 ggaccgcggc gccacctggg acttcaaggc gaacctgccg gtgacccaat tctacaagct
     2521 ggcgatcgac aacgccgagc cgttctataa cgtctacggc ggcacccagg acaactacac
     2581 gctcggcggc ccctcacgca cgttctcgac gcacgggatc accaatgccg actggttcgt
     2641 ggcgcaggaa ggcgacggct tccaggcgca ggtcgacccg aaggacccga acatcgtcta
     2701 cgccgaggcg cagcacggcg ccatcattcg tctcgatcgg aagaccggtg agcggatcga
     2761 catccagccg cagccggaca agggggagga tggctcgcgc tggaactggg actccccgct
     2821 catcatcagc ccgcactcca atacgcgact ctacttcgcg tcgcagcggg tgtaccggag
     2881 cgacgaccgg ggcgactcgt ggcgggcggt cagccccgac ctcacgcggc agatcgatcg
     2941 caaccgcctc aaggtgatgg ggcgggtgca gagcgtcgat tccatcgcca agaacgcctc
     3001 gacgtcgttc tacgggaaca tcgtggcgct ggccgagtcg ccgaagcggg agggccttct
     3061 ctacgtcggc accgacgacg gcctggtgca ggtgagcgct gactctggga agacctggcg
     3121 caaggccgag tcgttccccg gcgtccccga ccgcacctac gtgtcgcggc tgaccgcctc
     3181 gatccactcc gccggcaggg tgttcgccgc cttcaacaac cacaagatgg ccgacttcaa
     3241 gccctatctg ttgaagtccg acgacgaggg gaaatcgtgg acctcgatcg cgggggacct
     3301 gccggagcgc ggttcggtct gggctatcgc cgaggaccac gtcgatccgg cgctcctgtt
     3361 cgccgggacc gagttcggcc tcttcttcac ggtggacggc ggcgggcact gggtgcagct
     3421 gaaggggaag ttcccggtca tcccggtgcg cgacatcgcg atccagaagc gcgagaacga
     3481 cctcgtggtc gccaccttcg ggcgcggctt ctacatcctg gacgactaca cgccgctgcg
     3541 gggcttgcgc gcctcgacgc tggagcagga cgcggtcctc ttcccaatca agcaggcgcc
     3601 gctgtaccag ccgaccaact ggctcgggct caaggacaag gcgttccagg gggagtcgtt
     3661 cttcaacgcc ccgaatcccc ccttcggggc cgtgttcacc tactacctca aggacgagct
     3721 gctgacgcag aagaaggtcc ggcagaaggc ggagaaggaa accatcaaga acgccgggga
     3781 cgtcttctac ccgacctggg aggcgctgaa ggccgaggac cgggaggaag acccggtgat
     3841 cttgatgacc gtctccgatg cttccggaca ggtggtgcgc cagatcaccg gtcccgtgac
     3901 cgccggcttc caccgggtcg cctgggacct gcgctacccg ccgtcgcagc cggcgctcct
     3961 caaggtgccg gacccggagc cgttcgacga tccgcgccag gggccgttcg ctccgccggg
     4021 cgagtacacc gtgcgtctgg cgaagcgggt gaatggcgtc acgaccccgc tcgcagcccc
     4081 gatggtggtc gaggcggagg ggatcggcgc ctcgtcgctg ccgccggccg atcggcgcgc
     4141 cgtcgccgag tactcgcgca aggtcgcccg gctgcagcgg gccgtcctcg gcgccgagga
     4201 ggcggccggc gaggcgcaga accgcatcct cctcgtgaag cgggcgatcc aggacacgcc
     4261 gggggccgat ccgaagctgc tcgacgaggc gtgggcgatc gaggcgcggc tcaaagacct
     4321 gatggtcgcg ctgaccggcg atacgactcg ctcgcggcgc aacgagccca cttccccgtc
     4381 gatcagcgga cgcgtgaacc aggtggtggg cggcttctgg tccaccacct cggcgccgac
     4441 cgcgacgcac aagcgcaact acgacatcgc cgccgccgag ttcaccccgc tcctcgcgtc
     4501 gctgcgatct ctcatcgacg acgatctgcg gaagatcgag accgcggccg agaccgccgg
     4561 ggctccgtgg acgccgggcc gagtcccggt ctggtcagcg gagtaacgac acccccgctt
     4621 ccgtcacttc tccgaggagc acgtcgaggt ccgcctcggt cgtgcggtaa ttcacgaggc
     4681 aggcgcggag cgcgaaccgc ccgcgcaccc gggcgttact gacgaatgcg cggccgcgac
     4741 gctggactgc gagcaggacc gcctcgttca gccgggtgag gtccacctcg ggaagccttt
     4801 tgcccggcgg gacgtagcgg aagcagacga tcccgaggtc gccgcggctg agcagttcga
     4861 ggcggcgggt ggcgcggacc ttgtcggcga gcgcgcgggc gagctgcaga tcctcctcga
     4921 tcgaggcgga aaacgcctcg gcgccgtggt gcttgagggc gagccagatc ttgagcgccc
     4981 ggaagcggcg cgacagctcc gggccgtagt cccagaacgc cgtctgctcg agacgtccgg
     5041 cgccgaacat gcgggtgtag tcggcctcct ggcggaaggc gcggcgggcc gcctcggggc
     5101 gccgatagag gacgcagccg gcatccaccg gcaggtagag ccacttgtgc gggtcgaggg
     5161 cgatcgagtc ggcgaggctc agggcgcgca ggatcggccg ccccgagggc gtcaggatgg
     5221 cgaatccgcc gtagcaggcg tccacgtgca tccacagccg ctcgcgccgg cagaggcgcc
     5281 agacgtggcg cagcggatcg accgttcccg tggtgaccgt cccggcgctg gcgaccaccg
     5341 cgaacggcag gaggcgcgcc cggcgatcgc gctgcaccgc ttcgcggagc tgcgcgacgt
     5401 cgatccgcag ccggcgatcg accggcacga ggcgcacgaa gtcgcggccg atcccgagga
     5461 tcgatgccgc cttgaggacg gcgtggtgga cctcctccga ggcgtacatc accagcggcg
     5521 gccccggctc gccgcgcagc ccgcgccggg cgagatcgaa cggcaccgcc gcgtcgcgcg
     5581 ccgccgccag ccccgcgagg ttggcgaccg agccgccgct cgtcaggagc cctccggccg
     5641 atcgcggcag gccgaccatc tcgcggatcc agccgaccac ctgcatctcg accagtgcgg
     5701 cgccgggggc cgagcggaac gccgtcaggt tctggttgag gccggacgcc agccagtcgc
     5761 cgagcgctcc cgcggcgagt ccgggagggt tgacatagcc ccagaagcgc gggttgtcgt
     5821 tgtgccg
//
LOCUS       DASRIQ010000242        14366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1670449, whole genome shotgun sequence.
ACCESSION   DASRIQ010000242 DASRIQ010000000
VERSION     DASRIQ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 14366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14366
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670449"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VFD06_05980"
     CDS             <1..1127
                     /locus_tag="VFD06_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03119.1"
                     /translation="YTRVDVDPDFDHLEHAIAFSSLPFFHSFEAPILTPAPGDPICGE
                     AALASGWFEYTPAEDGRVQLYLQANTGHFHYVSLYERESGGLRAVACDRWSVRADVVA
                     GRTYDVMVGLPQPVPSFYLRVDPLPAPPPNDERDRPTVVTALPFHEEIDAGGARNEAG
                     DPGACGNQFVHNPNVWYAYTPAEDMRVTVDIPFDQTSVFRGPNEALVLLDCETDAPVT
                     FTALAGETYRVMTSQPTFPLSWPISVTISGRPAFGFGVTFDQTAGVNTRTGDAVVRGE
                     VTCARPARLHLEGELRQGSADRVGARGTFGVVVACDGRTGWTATVAPLPGAGARRRSG
                     FRNGAASIVIEAAGAPDDDPEDLAQRQVLGIILLRGGAAR"
     gene            1186..3918
                     /locus_tag="VFD06_05985"
     CDS             1186..3918
                     /locus_tag="VFD06_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZN03120.1"
                     /translation="MEFSFRPGMRAFAPPRARLALAVALVSLGAAAGDADRRSGAEIP
                     FVRPASRAASRADVATSDFVLRRRAVDLDLAALRAGSRRPGSRIRFDLFQDVALRGFV
                     DRVERRGTRGLTVTGRIAGEGTFVLVVERAQVSGSIRLPGRQFTIGADAGGRAIVEEV
                     DLERTSECTGAVVPPAAAQAPSVQEGKSPGGEAAPTPAAPDAGDLIDVLVAWTGRAET
                     AAGGPAAARALAQRAVDEANVAYGNSGIDTRLRLVWRGRTEYDESLDGHLAHLQRLVG
                     TGDGFMDEIHALRDATGADVVSLFVDDSEAAGLSYLMQSESTTFAGLAFSVVWFFAAS
                     NNLTLPHEIGHNQGCEHDRDHATSMPVFHDAYGMRFVGNDGRPYRTVMGYPPGQRLPN
                     FSNPAVFYQGRPTGRANKEDNAGAINATADTVANFRLARRHPGSLSDFDGNGADDLAT
                     FDALQGTLWIGLARPPVFDTMTWGAIPSSGAWQAHLRGDFDGDGWEDLASFDASIGAW
                     WVNRSRGDRFESLVWLQGAPGGTWLRHLTGDFDGNGRDDVASLDAAGILRVALSSGSG
                     FTPAAWGTLADPSAWAATIAGDYDGDGRDDLAHYDGTDGAWWVSLSTGTGFATSLWDR
                     HDAAAGWGDAVAGDFNGDGREDLAHLRSADGALWVEASTGAGFVPAAWAAFPPGGSWG
                     ALFAGDFDGDGLDDVAAFDAAEGGWWVGASNGGAFAVSPWSALQPATGWTRHEIGDFD
                     GDGRMDVADYLAARGEWWVGLSTGASFETSLWESFVLPRDDTDTDGDGLSDAADCDPT
                     ASDVWARPDGTDGLMLDQVGGPGSAARLQWQAPASIGGLLVHYDVLRSDSPWYFGWGS
                     VACLETNDAGDRQAVDPAPPAPIWYYLVRAGNACGEGSLGADSAGAPRQGVSCP"
     gene            complement(3931..4686)
                     /locus_tag="VFD06_05990"
     CDS             complement(3931..4686)
                     /locus_tag="VFD06_05990"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03121.1"
                     /translation="MFDAHAAQAEALYDSGRGADAERVYRGLLAQQPDHVASLVGLSQ
                     ILLDRDDLGGASVAIDRALAANPQSAPALRQRVAIQIARDQTKEAVATAQSAVRAHPG
                     SSPLHAALGDAERAFGDLPRAIAAYRKASSLNPEWAPGWTRLGEALAENGDAAEAEKA
                     LKRALALNPQSYPAYRALARMHYENGAPAQAVTEWEAALRLQPDRGEAHQGLAEALLA
                     AKRPAEARPHAEAAQRLGRDVQALMRQIDAAAK"
     gene            complement(4966..5784)
                     /locus_tag="VFD06_05995"
     CDS             complement(4966..5784)
                     /locus_tag="VFD06_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN03122.1"
                     /translation="MADEKDYVLGTHDEEIERLGLQHRVWRPRVLDAWKRAGFTVGQT
                     ILDVGCGPGYAALDLAEIVGPRGRVVAVDRSRRFLDALASAGRERGLAWIETREQDLD
                     LADLPSVGADAAWARWVIAFVRDPRALVGRIARALRPGGIFVSHEYFDYRTWRLLPRS
                     EEFERFVLQVMEVWRQSGGEPDIGLDLPRWLRDEGFEIRAIRPIVDVVSPASFVWHWP
                     RAFVEVGLRRLVDLGRLSASEAAAVQENFAARQAEPTTLMATPAVLETIAVRTT"
     gene            complement(5894..8398)
                     /locus_tag="VFD06_06000"
     CDS             complement(5894..8398)
                     /locus_tag="VFD06_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN03123.1"
                     /translation="MARGIVDGAAQDLKYAWRGLLKQPGFSALAILTLALGLGSVTAI
                     FSVVYGVLLQPLPYPRPDRLVAVWEVMPGGRRARLADPNFEDFRAQNRTFSALAKYSG
                     WTTAVTGLDEPVRAKVAAVSRDFFTVLGVHPVIGRTLGAEDAHPGAPAVAFLSDAFWR
                     AHVGAGGAPPTAADLPAIHLRIEGRDFAVAGVMPAGFAFPAADLWLPADLEPPNSSRT
                     SHNFSAIGRLRDGVTVEAAGADLDAIARGIARATAEQTDYLLSGAGAGPLRSVLAGRW
                     RTPLTLLLAAVGFLLLVACANVALFLLARASQRVRELAIRRALGAGHGRLVRQFVIEA
                     LLLTGLAAAAGTVLAAWALRLLLTLAPAELPRVAEIGLHAPVLGLAAGLSLAIGAALG
                     LVAAGRPTGFDAHGLTGALSEGGRGEAGGRRGRRAGRVLVGAQLAVTFVLLAGAALLG
                     RSLLEVLSIDPGFRTEGMLVMEVDPGSDPSEEADAVAAKARRSQLVSRLLERLAPIAG
                     VEQVAAASVLPLDGGLPDGMFLMVSERENPRTIEEYQALAKQPERRGTADFCLVTPGY
                     FAALGIPLRRGRDFDARDGLEAPHTAIISETLARRQWPDQDPLSRTLQFGNMDGDLHL
                     LTVVGVVADTRADGPEYPPRPIVYANLLQRPRAPFSVVMLAGEGSPATTAAARGILRS
                     EAPSAPPRFRTFESMYADALGYRRFNLLLVAAFALTALVLAVAGVYGVTAYGVARRTR
                     EIGVRMALGALPSQVARLILGEESRVALFGILAGAGGALILLRAMRTLLFGVSESDPF
                     TLVLVAALLLAVALLACFVPVRRASRIDPLEALREE"
     gene            complement(8512..9042)
                     /locus_tag="VFD06_06005"
     CDS             complement(8512..9042)
                     /locus_tag="VFD06_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03124.1"
                     /translation="MSRPAAERKEEAMGALAKRLVLGVLGVAVMLGYWTVKGWFVDDA
                     DATVSHIPAKIWDGGGGSIVLEAEATEAGRVSISFETNNAIDESDHKLLEAWERIGPG
                     LHTWTIEVPPDVSGTAEVDIDGPKVGSKARIAVKVNGRTAVEDRQTLDEPLKNGYAFF
                     AQVGLDDYAKGTPAED"
     gene            complement(9151..10431)
                     /locus_tag="VFD06_06010"
     CDS             complement(9151..10431)
                     /locus_tag="VFD06_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZN03125.1"
                     /translation="MGEKIAVIGLGYVGLPVALGFAKRFPGTVGFDINVEKVRSLRAG
                     SDITGEVPSETLRATTLRMTSDPVDLKEATFYVVAVPTPVDRNNRPDLTPLVKASQMV
                     GRALKKGDVVVYESTVYPGVTEEVCGPILENVSGLKRGDFRLGYSPERINPGDTLHTL
                     EKITKVVSGEDDATLDRVAAAYGAIVEAGVFRAASVKVAEAAKVIENTQRDLNIALMN
                     ELALIFDRMGIPTRDVLAAAGTKWNFLKFSPGLVGGHCIGVDPYYLTTKAEELGYQPQ
                     VILAGRRINNDMGPFVAQKTIKMLIHADLQVKGAKVGVLGLTFKENVADIRNSKVPDI
                     IHELRAFGIEPRLHDPYAGAEEARHEYGLGLSRLEDLEGLDALILAVAHKQYLDLGAE
                     ALARRVNPGGVLIDVKSALDPKQLPPGRVRYWSL"
     gene            complement(10461..12293)
                     /locus_tag="VFD06_06015"
     CDS             complement(10461..12293)
                     /locus_tag="VFD06_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZN03126.1"
                     /translation="MARTSLLACEGVSKSFGGPPLFEGLTFVVHDGDHVGLVGPNGAG
                     KSTLLKILGGLETPDSGSCARRKNLRIGYVPQTPVFESGRTAGAIVAEAIATDPHVDE
                     HERDERAERALRQAGFADPDVLIDHLSGGWRARLALTREIARSPDLLLLDEPTNHLDI
                     ESILWLEQLLENAPGAFVAVSHDRYFLERTARRMLEIDRLHESGLFQANGTYADFLEA
                     RDAALSREAAYLDTLAGLVRREVAWLRRGARARTTKSKARIASAGRSIERLEEGRART
                     AVTTAAIEFTASGRKSKRLWAAEGLRIAFGERTIIEGLDLVLTPGLRLGVVGPNGSGK
                     TSLLRTIVGEIPPAGGSVQPAEALRVVYFAQNRETLDPRLSLKRALAPEGDTVVYGDR
                     ALHVASWAKRFLFQPGQLETPVSRLSGGERARIVLARMMLRPADLLVLDEPTNDLDIP
                     ALEVLEEALLEFPGALVLVSHDRHLLDRVSTRILALDGRGSAQHFADYAQWEEARPPA
                     NRLGKEAPPLSPTRPAPRPAAPGAPRPVTRRLGYLEQREWDGLEAAVHEAETRLAEAR
                     RSAEDPSIATDAALLAKRLSALQGLQAEVDRLYARWAELEEKQR"
     gene            complement(12310..>14366)
                     /locus_tag="VFD06_06020"
     CDS             complement(12310..>14366)
                     /locus_tag="VFD06_06020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN03127.1"
                     /translation="AAQSPAPPAAAPPSAAAKAAAPPAAGRFQPMDMFGLEWASDPQI
                     SPDGRRVAYVRTSLDVMKDLRRSQIWLVNADGTDHRPLTSGAGEFAPRWSPDGRRLVY
                     AARDGDSMQLFVRWMDTGATTRLAQLQRAPGGVAWSPDGKSIAFTMLVPAKPEPLAKM
                     PEKPEGADWAKPPRVITRLVFRADGEGFIEEGFSHLFVVPSEGGTPRPISSGSFHHDG
                     APAWTADGKALLISANRRDDWEYESADSEIYEFAVADGAMRRLTDRRGPDTSPVVSPD
                     GRRIAYLGYDDRYQGYQVTRLYMMNRDGSGMRLLTGGYDRDIEQPMWSRDGKRILFAT
                     ADRGNGKIGSIAVDGAAGAKPEILAADLGGGDLGRPYSGGSYSVARADDRIAYTWTRP
                     DRPPEVAVAAPGAKTAVLTSLNDDLFAGKTLATVEEIWTESSFDHRRVQGWIMKPPGF
                     DPAKKYPMILEIHGGPFADYGDRFGAELQLYAAAGYVVLYGNPRGSTSYGEEFGNLIH
                     HAYPGNDYDDLMSLVDATVGRGYVDPANLFVTGGSGGGVLTAWIVGKTDRFRAAVSAK
                     PVINWYSFVLTADETNYFTRYWFASPPWENPQEYLKRSPLSYVGNVKTPTMLLTGEAD
                     YRTPIAEAEQFYEALKMRKVDTALVRVPDASHGMFERPSMLLGKSAHILAWFEKYRKP
                     AA"
BASE COUNT         2059 a         5093 c         5129 g         2085 t
ORIGIN      
        1 tctacacgcg cgtcgatgtg gaccccgact tcgaccatct ggagcatgcc atcgcgtttt
       61 cgtccctccc cttcttccat tcctttgaag cgccgatcct cacgccggcc cccggcgatc
      121 cgatctgcgg cgaggcggct ctggcgtccg gctggttcga atacacgccg gcggaagacg
      181 gacgcgtcca actgtacctg caggcgaaca ccggtcattt ccactacgtt tcgctttacg
      241 aaagggagag tgggggcctg cgcgccgtgg catgcgaccg gtggagcgtg cgcgccgatg
      301 tcgtcgccgg ccggacctat gacgtgatgg tcggtctgcc gcagccagtt ccctcctttt
      361 acttgcgggt cgaccctctg cccgcgccgc cgcccaacga cgagcgcgat cgccccaccg
      421 tcgtgacggc gctccccttc cacgaagaga tcgatgcagg cggcgcgcgc aacgaagcgg
      481 gagaccccgg agcctgcgga aaccagttcg ttcacaatcc gaacgtctgg tacgcctaca
      541 cgcccgccga agacatgcgg gtcaccgtcg acatcccatt cgaccagacc tcggtcttcc
      601 gcggtccgaa cgaggcgctg gtcctgctgg actgtgaaac ggacgccccc gtcaccttca
      661 cggcgcttgc cggagagacc taccgcgtca tgaccagcca gccgaccttc ccgctgtcgt
      721 ggcccatttc cgtcaccatc agcggccggc ccgcgttcgg cttcggagtg acgttcgacc
      781 agaccgccgg ggtgaacacg aggaccggcg acgcggtggt ccgcggggag gtgacctgcg
      841 ctcgccccgc gcgcctccac ctggaaggcg aattgcgcca gggctccgcg gatcgcgtcg
      901 gagcgcgcgg cacgttcgga gtggtggtgg cgtgcgacgg aaggaccggc tggaccgcga
      961 ccgtcgcgcc tcttcccgga gccggcgcac gccgccgctc cggcttccgc aacggtgccg
     1021 cgagcatcgt gatcgaggcc gccggagccc cggacgatga cccggaggac ctcgcgcagc
     1081 gacaggtcct gggaatcatc ctccttcggg gcggcgcggc gcgctgagct gcgcttgatc
     1141 gcggggccgc gcccggacta gagtcaagcg catcgagcca tcgcgatgga gttttccttc
     1201 cgcccaggga tgcgggcgtt cgcgccgccg cgcgccaggc tggccctggc cgtcgcgctc
     1261 gtgtcgctcg gcgcggccgc gggtgacgcc gatcggcgtt ccggcgcgga gatcccgttc
     1321 gtgcgtcccg cttcccgcgc cgcgtcgcgg gccgacgtcg cgacctccga cttcgtcctc
     1381 cgccggaggg ccgtggatct cgacctggcc gcgctgcgcg cgggatccag gaggccgggg
     1441 agccggatcc gcttcgacct cttccaggac gtcgcgctgc gcggcttcgt cgatcgcgtc
     1501 gagcggcgcg gcactcgtgg cctgacggtc accggtcgaa tcgccggcga agggaccttc
     1561 gttctcgtcg tcgagcgggc gcaggtctcg ggaagcatcc gcttgccggg ccggcagttc
     1621 accatcggcg cggacgccgg tggtcgcgcg atcgtcgagg aggtcgacct cgaacgcacg
     1681 agcgaatgca ccggggcggt ggttccgccc gccgcggcac aggcgccctc ggtccaggaa
     1741 gggaagtcgc cgggagggga ggcggcgccc accccggcgg cgccggacgc tggcgacctc
     1801 atcgacgtcc tcgtcgcgtg gaccggccgt gccgagacgg cggcgggcgg gccggccgcg
     1861 gcgcgcgccc tggcgcagcg cgcggtggac gaggcgaacg tcgcctacgg caacagcggc
     1921 atcgacacgc ggctgcggct cgtctggcgc ggccggacgg agtacgacga gagcctcgac
     1981 ggccacctgg cccatctcca gcgcctcgtc ggcacgggcg acggcttcat ggacgagatc
     2041 cacgcgctca gggacgcgac cggggccgac gtcgtcagcc tgttcgtgga cgactcggag
     2101 gcggcggggc tcagctacct gatgcagtcg gagtcgacga ccttcgccgg actggccttc
     2161 agcgtcgtct ggttcttcgc cgcctccaac aacctgacgc tcccccacga aatcgggcac
     2221 aaccaggggt gcgagcacga tcgcgatcac gccacctcga tgccggtgtt ccacgacgcc
     2281 tacggcatgc gcttcgtcgg caacgacggc cgcccgtacc ggaccgtgat ggggtatccg
     2341 cccgggcagc gccttccgaa tttctccaac ccggcggtct tctatcaggg gcggccgacc
     2401 gggcgcgcca acaaggagga caacgccggc gccatcaatg cgacggcgga tacggtcgcg
     2461 aacttccgtc tcgcccggcg ccatcccgga tcgctgagcg acttcgacgg caacggcgcc
     2521 gacgacctgg cgaccttcga cgcgctgcag gggacgttgt ggatcggtct cgctcgtccg
     2581 ccggtgttcg acaccatgac gtggggcgcg attccgtcct ccggcgcatg gcaggcgcat
     2641 ctccgcggag acttcgacgg cgacggctgg gaggacctgg cgagcttcga cgcttcgatc
     2701 ggcgcctggt gggtcaaccg ctcgcgcggc gatcgcttcg agagcctcgt ctggctccag
     2761 ggcgcgcccg ggggcacgtg gctgcggcac ctgaccggag acttcgacgg caacggcagg
     2821 gacgacgtcg cgagcctcga cgccgcgggg atcctgcgcg tcgcgctctc gagcggcagc
     2881 gggttcacgc cggccgcgtg gggaacgctc gccgatccgt ccgcctgggc cgcaacgatc
     2941 gccggcgact acgatggcga cgggcgggac gacctggcgc actacgacgg gacggacggc
     3001 gcgtggtggg tcagcctctc cacgggaacc ggcttcgcca cgtccctctg ggatcggcac
     3061 gacgccgcgg cggggtgggg cgacgcggtc gccggggatt tcaatgggga cggccgggag
     3121 gatctggcgc acctccgttc cgcggacggc gccctgtggg tcgaggcctc caccggcgcc
     3181 ggattcgtcc ccgcggcgtg ggcggcgttc ccgcccgggg gatcgtgggg ggcgttgttc
     3241 gccggagatt tcgacggcga cggcctcgac gacgtcgcgg cgttcgacgc ggccgaagga
     3301 gggtggtggg tcggtgcctc gaacggcggc gcgttcgcgg tttccccatg gtcggcgctg
     3361 cagccggcga ccggatggac ccggcacgag atcggcgact tcgacggcga cgggaggatg
     3421 gacgtcgccg actatctcgc ggcgcgcggc gagtggtggg tcggcctctc caccggagcc
     3481 tcgttcgaga cgtcgctctg ggagagcttc gtcctgccgc gcgacgacac ggacaccgac
     3541 ggcgacggtc tctccgacgc cgccgactgc gacccgacgg cgtccgacgt ctgggcccgt
     3601 ccggatggga cggacgggtt gatgctcgat caggtcgggg gaccgggaag cgccgcgagg
     3661 ctgcagtggc aggcgccggc gtcgatcggg gggctcttgg ttcactacga cgtgctgcgc
     3721 tcggactccc cgtggtattt cggctggggc tcggtcgcct gcctcgagac caacgacgcc
     3781 ggcgatcgcc aggcggtgga tcccgcgccg cccgctccta tctggtacta cctcgtccgg
     3841 gccggcaacg cctgcggtga ggggagcctc ggcgccgact cggccggagc gccgcgccag
     3901 ggcgtgtcct gtccctgata cggcgccggc ttacttcgcg gcggcgtcga tctgccgcat
     3961 cagcgcctgg acgtcgcgcc cgagacgctg cgccgcctcg gcgtggggac gggcttcggc
     4021 cggacgcttc gcggcgagga gcgcttcggc cagcccctga tgcgcttcgc cgcgatccgg
     4081 ctgcaggcgc agcgccgcct cccattcggt cactgcctgc gccggcgcgc cgttctcgta
     4141 gtgcatcctg gccagtgctc ggtacgcggg gtacgactgc gggttgagcg cgagcgcccg
     4201 cttcagcgcc ttctccgcct ccgcggcgtc gccgttctcc gccagcgcct ccccgagccg
     4261 cgtccagccg ggggcccact cggggttcag cgaggaggct ttccggtagg ccgcgatcgc
     4321 cctggggaga tcaccgaagg cgcgctcggc gtcaccgagc gcggcgtgga gcggggagga
     4381 gccgggatgc gcccgcacgg cgctctgcgc cgtcgccacc gcttccttcg tctgatcgcg
     4441 ggcgatctgg atcgcgactc tctgccgcag cgccggggcc gactgcgggt tggccgcgag
     4501 cgcccggtcg atggccacgg aggcgccgcc gagatcgtcc cgatcgagca ggatctgcga
     4561 cagcccgacg agcgacgcca cgtgatcggg ctgctgcgcg aggagtccgc ggtaaacccg
     4621 ttcggcatcc gcgccacggc cgctgtcgta caaggcctcc gcctgcgccg cgtgcgcgtc
     4681 gaacatctcc gcgacggccg tgcccggcgc agcggaatcc gcggaagcgg ccgcggacgg
     4741 tgccgcaggg tccggagtgg cggacgatgg agccgaggca cccgggggcg ccgccgccgt
     4801 gcgctcggcg gcatccggcg gcgtctgcgg cttctccccg caggaagcgg ccgcgatcgc
     4861 gaggaccagg agcggcgcga tcctccctgc atgctttccc cacgcggcca ggcacgacgg
     4921 cttcggcatc ggagtcctcc cggcgatcgt tcgccgggga cgattctaag tcgttcggac
     4981 ggcgatcgtc tcgaggacgg caggcgtggc catcaacgtc gtcggctcgg cctgacgggc
     5041 ggcgaagttc tcctgcacgg ccgccgcctc gctggcgctc agtcggccga gatcgacgag
     5101 ccgtcgcaga ccgacctcca cgaaggctcg cggccagtgc cagacgaacg aggcagggga
     5161 gaccacgtcg acgatggggc ggatcgcgcg gatctcgaat ccttcgtcac ggagccagcg
     5221 cggcagatcg agcccgatgt ccggctcgcc gccgctctgc cgccagacct ccatgacctg
     5281 gagcacgaag cgctcgaact cctccgaccg cggcaggagg cgccaggtgc ggtagtcgaa
     5341 gtactcgtgc gacacgaaga tgccgcccgg ccggagggcg cgggcgatgc ggccgacgag
     5401 ggcgcgcggg tcccgcacga aggcgatcac ccagcgcgcc caggcggcgt cggccccgac
     5461 cgacggaaga tccgccaggt cgagatcctg ctcgcgcgtc tcgatccagg cgaggccgcg
     5521 ctcccggccg gccgaggcga gggcgtcgag gaagcgccgc gatcgatcga ccgccaccac
     5581 ccgcccgcgc ggcccgacga tctccgccag gtcgagcgcc gcgtaccccg gcccgcagcc
     5641 gacgtcgagg atcgtctgcc cgacggtgaa ccccgcgcgc ttccaggcat cgagcacccg
     5701 cggccgccag acgcggtgct gcagaccgag ccgctcgatc tcctcgtcgt gcgtcccgag
     5761 gacgtagtcc ttctcgtccg ccatcgccac ctccgggaga gtttacgcac cccggaggcg
     5821 tttggggtgg aagcggggtc acgtctcggc gttgacgttc ccgttcggtc ccagttcaag
     5881 gcggcgaagc cggctattcc tcccgcagag cctcgagcgg atcgatgcgc gaagcgcgcc
     5941 gcaccgggac gaaacacgcg agcagggcca cggccaggag gagcgccgcg acgaggacca
     6001 gcgtgaacgg gtcgctctcg ctcaccccga acagcagcgt ccgcatggcg cgcaggagga
     6061 tcagcgctcc gccggctccg gcgaggatgc cgaaaagcgc cacgcgcgac tcttcaccca
     6121 ggatcaagcg cgccacctgc gacggcaggg cgccgagcgc catgcgcaca ccaatctcgc
     6181 gggtgcgccg cgccaccccg tacgcggtga cgccgtagac gccggcgacg gcgagcacga
     6241 gcgccgtcag cgcgaaggcg gcgacgagga ggaggttgaa gcgccgatat cccagcgcgt
     6301 cggcgtacat cgattcgaag gtgcggaagc gcggcggcgc cgacggcgcc tcggacctca
     6361 agataccgcg ggccgccgcc gtggtcgcgg gcgagccctc cccggcgagc atcaccacgc
     6421 taaagggggc gcgggggcgt tgcaggagat tggcatagac gatcgggcgg ggaggatact
     6481 cgggcccgtc cgcgcgcgtg tcggcgacga caccgaccac cgtcaggagg tgcaggtcgc
     6541 cgtccatgtt gccgaactgc agcgtccggc tcagaggatc ctgatccggc cactgcctgc
     6601 gggccagcgt ttcgctgatg atggcggtgt gcggcgcctc gagcccatcg cgggcatcaa
     6661 agtcccggcc cctccggagg ggtatgccga gcgccgcgaa ataacccggc gtcaccaggc
     6721 agaagtcggc cgtgccacgg cgctccggtt gcttcgccag cgcctggtat tcctcgatcg
     6781 tccgtgggtt ctccctctcg ctcaccatca ggaacatccc gtccggcagc ccgccgtcga
     6841 gcggcaggac gctggcggca gcgacctgct ccacaccggc gatcggagcc aggcgttcca
     6901 gaagccggct gaccagctgc gaccgccggg ccttcgccgc caccgcgtcc gcctcctcgg
     6961 aggggtcgga tcccggatcg acctccatca ccagcatccc ttcggtgcgg aaaccgggat
     7021 cgatcgacag cacctcgaga aggcttcgcc cgaggagcgc cgcgccggcg aggagcacga
     7081 aggtgactgc cagctgcgcg ccgacgagga cgcgtccggc tcgccggccg cggcggccgc
     7141 cggcctcccc ccgccctcct tcgctcagcg cccccgtgag gccgtgcgcg tcgaacccgg
     7201 tcgggcgccc cgcggccacg agcccgaggg ccgcgccgat cgccagcgac aggccggcgg
     7261 cgagacccag cacgggtgcg tgcaggccga tctcggcgac ccgcggaagc tcggcggggg
     7321 ccagcgtcag gagcaggcgc agcgcccagg cggcgagcac ggtccccgcc gcggccgcca
     7381 gccccgtcag cagcagcgcc tcgatgacga actggcgcac cagacgcccg tgcccggcgc
     7441 cgagggcgcg gcggatcgcc aactcccgca cccgctgcga agcccgcgcc agaaggaaca
     7501 gtgccacgtt ggcgcacgcg acgagcagga ggaatccgac cgcggcgagg aggagcgtca
     7561 gcggagtgcg ccaccgcccg gcgagcaccg agcgtagcgg cccggccccc gccccgctca
     7621 ggaggtagtc ggtctgttcg gccgtggcgc gggcgattcc gcgggcgatg gcgtcgaggt
     7681 cggcgccggc ggcctctacg gtgacgccgt cgcgcagccg gccgatggcc gagaagttgt
     7741 gggaggtgcg gctggaattc ggaggctcga gatcggccgg cagccacagg tcagcggccg
     7801 ggaaggcgaa cccggccggc atcacccccg cgactgcgaa gtcacgtcct tcgatgcgaa
     7861 ggtggatcgc cggcagatcc gccgccgtcg gcggcgcgcc gccggcgccc acgtgcgccc
     7921 gccagaaggc atcgctcagg aacgccaccg ccggcgcccc cgggtgcgcg tcctccgcgc
     7981 cgagggtgcg tccgatgaca gggtgaacgc caagcacggt gaagaaatcg cgcgacacgg
     8041 cggcgacttt ggcccgcacc ggctcgtcga ggccggtcac cgccgtcgtc catccggagt
     8101 acttcgccaa ggccgagaag gtgcggttct gcgcgcggaa gtcctcgaag ttcgggtcgg
     8161 cgaggcgcgc ccgtcggcca cccggcatga cttcccacac tgccacgagg cgatcgggcc
     8221 gcgggtacgg cagcggctgc agcagcacgc cgtacaccac gctgaagatc gccgtgaccg
     8281 acccgagccc caacgccagc gtcaggatgg cgagggcgct gaagcctggt tgcttgagga
     8341 gtccgcgcca ggcgtacttc aggtcctgcg ccgccccatc gacgatgccg cgcgccatga
     8401 cgatccctcg gcgatccacg tctgcgtggg gaaggatacg gcgggaccgg cggaaaaggt
     8461 tccgtcaagg gtggaaggcg ttcgaccggc gggctgggtc cagcggggag gctagtcttc
     8521 ggcgggcgtc cccttcgcgt agtcgtcgag gcccacctgg gcgaagaagg cgtatccgtt
     8581 cttgagcggt tcgtcgagcg tctggcggtc ctcgacagcg gtccggccgt tgacctttac
     8641 ggcgatgcgc gctttcgatc cgaccttcgg gccgtcgatg tccacctcgg ccgtccccga
     8701 cacgtccggt ggaacctcga tggtccaggt gtgcaggccg ggcccgatcc gctcccaggc
     8761 ctcgagcagc ttgtgatccg actcgtcgat cgcgttgttc gtctcgaagg agatcgagac
     8821 gcgccccgct tcggtcgcct ccgcttccag gacgatgctc ccgccgcctc cgtcccagat
     8881 cttggcgggg atgtgggaga cggtcgcgtc cgcgtcgtcg acgaaccatc ccttgaccgt
     8941 ccagtatccg agcatgaccg cgactcccag gacgcccagg accagccgct tcgccaacgc
     9001 tcccatcgcc tcctccttgc gctcggccgc cggtcgggac acacctcccg cgccggggcg
     9061 gagcggggcg gaaacatagc gacccgagcg gcgcggatca agccagaaga cggcggtctt
     9121 tcgatggaaa cttggatccg tggcggtgcg tcagagggac cagtagcgga cacggcccgg
     9181 gggcaattgc ttcggatcga gggcgctctt cacgtcgatc agcacgccgc cggggttgac
     9241 gcgccgggcc agcgcctccg cgccgaggtc gaggtattgc ttgtgcgcca ccgcgaggat
     9301 gagcgcgtcc agcccctcga gatcttcgag ccgcgagagg ccgagcccgt attcgtgccg
     9361 cgcttcctcg gcgccggcgt acggatcgtg gagccggggt tcgatcccga aggcgcgcag
     9421 ctcgtggatg atgtcgggca ccttcgagtt cctgatgtcc gcgacattct ccttgaaggt
     9481 cagcccgagc accccgactt tggctccctt cacctgcagg tcggcgtgga tcagcatctt
     9541 gatcgtcttc tgcgcgacga acggtcccat gtcgttgttg atccggcggc cggccaggat
     9601 gacctggggc tgatagccca gctcctcggc cttcgtcgtc aggtagtagg gatcgacgcc
     9661 gatgcagtgg ccgccgacga gaccgggcga gaacttcagg aagttccact tggtgccggc
     9721 cgcggcgagc acgtcgcggg tcgggatccc catccggtcg aagatgagcg ccagctcgtt
     9781 catcagggcg atgttcaggt cgcgctgggt gttctcgatc accttcgccg cctcggccac
     9841 tttcaccgag gccgcccgga acacccccgc ctcgacgatc gcgccgtagg cggccgccac
     9901 tcgatccagc gtcgcgtcat cctcgcctga gacgaccttg gtgatcttct cgagcgtgtg
     9961 cagggtgtct ccggggttga tccgctccgg cgagtagccg agccggaagt cgccccgctt
    10021 cagccccgag acgttttcga ggatggggcc gcagacctcc tccgtgacgc ccgggtagac
    10081 ggtggactcg tagaccacga catccccctt cttcagggcg cgccccacca tctgcgacgc
    10141 tttcaccagc ggggtcagat cgggacgatt gttccggtcg acaggcgtcg ggacggccac
    10201 gacgtagaac gtggcctcct tcaggtcgac cgggtcggag gtcatccgaa gcgtggtcgc
    10261 ccgcagtgtc tcggacggca cctcgccggt gatgtcgctc ccggcacgga gcgaccgcac
    10321 cttctcgacg ttgatgtcga accccacggt tccggggaac cgcttcgcga agccgagcgc
    10381 gaccggaagc ccgacgtatc ccagcccgat gactgcgatt ttctcgccca tgggagcggt
    10441 ggattatagc gtcgcgcgcg tcaacgttgt ttttcctcga gctccgccca gcgggcgtag
    10501 agccggtcga cctcggcctg aagaccctgg agcgccgaca gacgcttcgc gagcagcgcc
    10561 gcgtcagtcg cgatcgacgg gtcctcagcg ctccgccgcg cttccgcgag ccgcgtctcc
    10621 gcttcgtgca ccgccgcctc caggccgtcc cattcgcgct gctcgagata gccgagacgc
    10681 cgcgtcacag ggcgcggcgc cccgggtgct gcggggcgcg gcgccggccg agtcggcgac
    10741 aggggcggcg cctccttccc gaggcggttc gcaggcggcc gggcctcctc ccactgtgcg
    10801 tagtcggcga aatgctgggc gctgccgcgg ccgtcgaggg cgaggatccg cgtcgagacc
    10861 cgatcgagca ggtggcgatc gtggctgacg aggacgagcg cgccgggaaa ctcgagcagc
    10921 gcttcctcca ggacctcgag cgccgggatg tcgagatcgt tcgtcggctc gtcgaggacg
    10981 agcagatcgg ccggacgcag catcatccgg gcgaggacga tccgggcgcg ctcgccgccg
    11041 gacaggcggg acaccggggt ttcgagctga ccgggctgga agagaaagcg cttcgcccag
    11101 gacgcaacgt gcagcgcccg gtcgccatag acgacggtgt ccccctcggg agccagcgcc
    11161 cgcttgagcg agagtcgcgg gtcgagcgtc tcccggttct gggcgaaata gaccacccgc
    11221 agcgcttcgg ccggctgcac cgatccgccc gcgggcggga tctccccgac gatggtgcgg
    11281 aggagcgacg tcttgccgga tccgttggga ccgacgacgc cgagccgcag gccgggcgtc
    11341 agtacgagat cgaggccctc gatgatcgtc cgctcgccga aggcgatgcg caggccctcc
    11401 gcagcccaga gccgcttcga ctttctcccg gaggcggtga attcgatggc ggcagtcgtc
    11461 accgcggtcc gcgcccgccc ctcctcgagc cgctcgatgc tgcgcccggc cgaggcgatg
    11521 cgcgccttcg acttggtggt gcgcgcccgg gcgccgcggc gcagccaggc cacctcgcgc
    11581 cgcaccagcc cggcgagcgt gtcgagatag gccgcctccc ggctgagcgc cgcgtcacgc
    11641 gcctcgagga aatcggcgta ggtgccgttc gcctggaaga gccccgattc gtggagacgg
    11701 tcgatctcca gcatccgccg cgccgtccgc tcgaggaaat agcggtcgtg gctgaccgcg
    11761 acgaaggccc ccggcgcgtt ctcgagcaac tgctcgagcc agaggatcga ctcgatgtcg
    11821 aggtggttgg tcggctcgtc gagtagcagc aggtccggcg agcgggcgat ctcgcgcgtc
    11881 agggcgagcc gggcgcgcca ccctcccgac aggtggtcga tcaggacgtc cgggtcggcg
    11941 aaccccgcct gccgcagcgc ccgctccgcg cgctcgtccc gttcgtgctc gtccacgtgc
    12001 ggatcggtcg cgatcgcctc cgcgacgatg gcgccggccg tccggcccga ctcgaagacc
    12061 ggggtctgcg ggacgtagcc gatgcggaga ttcttccggc gggcgcagga acccgagtcg
    12121 ggggtctcga gcccgccgag gatcttgagg agcgtggatt tcccggcgcc gttcgggccg
    12181 acgagcccga cgtgatcccc gtcgtgcacg acgaacgtca gcccttcgaa cagcgggggc
    12241 ccgccgaacg acttggagac gccttcgcag gcgagaagag aggtgcgggc catgcggggt
    12301 ggcagcctct caggcggccg gcttccgata tttctcgaac cacgcgagga tgtgggccga
    12361 cttgccgaga agcatgctcg ggcgctcgaa catcccgtgc gacgcgtccg gcacgcgcac
    12421 gagcgccgtg tcgaccttcc gcatcttgag cgcctcgtag aactgctccg cctcggcaat
    12481 cggcgtgcga tagtcggcct cgccggtcag gagcatcgtc ggcgtcttga cgttgccgac
    12541 gtacgacagc ggcgagcgct tcaggtactc ctgagggttc tcccatggcg gcgaggcgaa
    12601 ccagtagcgg gtgaagtagt tcgtctcgtc ggcggtcagg acgaagctgt accagttgat
    12661 caccggcttg gccgagaccg cggcgcggaa gcggtcggtc ttgccgacga tccaggcggt
    12721 caggacgccc ccgccgctgc cgccggtcac gaagaggttc gccggatcga cgtatccccg
    12781 gccgaccgtc gcgtccacca ggctcatcag atcgtcgtag tcgttccccg ggtaggcatg
    12841 gtggatgagg ttgccgaact cctcgccgta cgaggtcgag ccgcgcgggt tgccgtagag
    12901 gacgacgtac ccggccgcgg cgtagagctg cagctcggcg ccgaagcggt cgccgtaatc
    12961 ggcgaacggc ccgccgtgga tctcgaggat catcgggtac ttcttcgccg ggtcgaaccc
    13021 gggcggcttc atgatccagc cctgcacgcg gcgatggtcg aacgaagact cggtccagat
    13081 ctcctccacc gtggccagag tcttgccggc gaagaggtcg tcgttgagcg aagtgaggac
    13141 cgccgtcttc gcgccggggg ccgcgaccgc cacctccggc ggccggtcgg gacgggtcca
    13201 ggtgtaggcg atgcgatcgt cagcgcgcgc caccgagtac gagccgccgg agtacgggcg
    13261 gccgaggtcg ccgccgccga gatccgccgc gaggatctcc ggcttcgcgc cggcggcgcc
    13321 gtcgaccgca atcgacccaa tcttcccgtt gccgcggtcg gcggtggcga agaggatgcg
    13381 cttgccatcg cggctccaca tcggctgctc gatgtcccgg tcgtaaccgc cggtgaggag
    13441 gcgcatccct gagccgtccc ggttcatcat gtacaggcgc gtgacctggt acccctggta
    13501 gcgatcgtcg tacccgaggt acgcgatgcg ccggccgtcg ggggagacga cgggtgacgt
    13561 gtcggggccg cggcgatcgg tcagccggcg catcgcgccg tcggccacgg cgaactcgta
    13621 gatctccgag tccgccgact cgtactccca gtcgtcccgg cggttcgccg agatgagcag
    13681 ggcttttccg tctgccgtcc aggccggcgc cccgtcatgg tgaaaggacc cggacgagat
    13741 cggccgcggc gtccctcctt cggaggggac cacgaaaaga tggctgaacc cctcctcgat
    13801 gaacccctcg ccgtcggccc ggaacacgag gcgcgtgatc accctggggg gcttcgccca
    13861 gtccgcgcct tccggcttct cgggcatctt cgccagcggc tccggcttcg ccgggacgag
    13921 catggtgaag gcgatcgatt ttccgtccgg agaccacgcg acacctcccg gcgcgcgctg
    13981 cagctgggcc aggcgcgtgg tggcaccggt gtccatccag cggacgaaca attgcatcga
    14041 gtcgccgtcg cgcgccgcgt agacgagccg gcggccgtcc ggcgaccagc gcggcgcgaa
    14101 ctcgccggcg ccggacgtca gcggccggtg atccgttccg tcggcgttca cgagccagat
    14161 ctgcgagcgt cgcagatctt tcatgacgtc caacgaggtg cgcacgtagg cgacccgccg
    14221 tccgtcgggg gagatctgcg ggtcggacgc ccattccagc ccgaacatgt ccatcggctg
    14281 gaagcggccg gcggcgggcg gcgccgcggc cttcgctgcg gcggatggcg gcgcggccgc
    14341 cgggggagct ggggactggg ccgcgg
//
LOCUS       DASRIQ010000243         5471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3767629, whole genome shotgun sequence.
ACCESSION   DASRIQ010000243 DASRIQ010000000
VERSION     DASRIQ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3767629"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFD06_06025"
     CDS             complement(<1..535)
                     /locus_tag="VFD06_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03128.1"
                     /translation="MLLTCAVAAGALALAALVARPWLARLVAEHARQEAVEKAAAALG
                     APVSIARSTAGFAPVVVILEGLRLEADGAFGLRAGSSVERVEISGDPWALVRWGSGPI
                     DFHVERPHCVVSVAAAAGAAAPGAGVSGGGAAPGPTVPPAVAAPSSGPPATQSSTQTV
                     KSAEVASPGGAAPAFPAG"
     gene            complement(593..1816)
                     /locus_tag="VFD06_06030"
     CDS             complement(593..1816)
                     /locus_tag="VFD06_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03129.1"
                     /translation="MTPTPPRDPRPRRRFFLPLAAIAPGVREAAASKGLLGHLWPMPS
                     VVFSAFMIAWGAECAQFWVSRGMALAILAWLQALPEFAVEAVIAWSQQIHFMTANFTG
                     SLRLLVGLGWPLIFSVAAFGAWRREKRLLRQITLDRENSIEVFALGLPILYFLFIWLK
                     ASLTLVDAGLLLAIYFLYLWVLRKIPPREEEHVDDLEAVPRRIMRLPKRAQGFAILGL
                     FVGGGLLLAYSAHPFLESMLHFAVTLGVSEYLFIQWVAPFLSEFPEKLSAMYWARRPG
                     KAAMALMNMVSANINQWTMLAAMIPVVYSMSVGTPTFVPFDDHQRAEILLTISQSLLG
                     MLLLMNMSFHIVEAGGIFLLWFAQFLVPGLHLPVTLIYLGWSAYEAFMMIVVRRRVEA
                     WHALSEVWREHGRRR"
     gene            complement(1846..4542)
                     /locus_tag="VFD06_06035"
     CDS             complement(1846..4542)
                     /locus_tag="VFD06_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZN03130.1"
                     /translation="MNRRSRALLVSLPLLAAIVLPAAAEDPAPDPAAVEARQNAAVTA
                     AVNGLVQEGNKARREGRLQAALDAYRSASGLDPKRYEIRILIADTLRRVGRVSEAAAA
                     YEAATTLAPGRAEGYTGQAILKRAEYDFDGAEALLLPALKKVQGAERADLLVTLGETR
                     RREGKTKDAEESFREGLATDPKNAPAQAGLARLAEERGDLDGAVAAWDRFLALAPDDA
                     PARARRDELQEVRALLAALGAAAKTSGSGRAGAEVWGEIGRLRAIAGDAPGAAEAYRA
                     ALKRAPGDNGLRRGLALALEAAGDEAGAGAEFAKLLRAAPGDPIALYHQAALARRRGD
                     ADAEERAWRDLVAAHPDDLYATRALAAFVERAGADARGRVLAAAPAGISGLRLRALLL
                     ADAGAWTDVEIALEAALRIDSTEPWTLDVFTDLLGRRPDMLAHLAARAQKDLAAAAAD
                     PTPSLLVLARCHLLAGHPGEARAILDRVVAARPNLAVPRSALAETQQGAGRDRSAAIA
                     SLEKAIALDPKRLAPHVDLALLHLRLRRAAESEQAARRGLESHPEAAPLLSLLGAARA
                     DQDDAEGAARFYARALAADPADNFHLARSQYPAVLASLGRTFEARRALGGMLPALPDL
                     QYLEAWAFARDSFRDRTFHGQDWNAARARHRELAVAPGSAALRAIALLLATLGDPYTR
                     LRDPEETSAVWLTRHQGPRETDALGRNRAGGATVTTEELPGNLGYVQIANFTDPNAVR
                     ELRRALAALGDKSGLVLDLRGNAGGLTRSADEVADLLIGPGVEVGSESGPEGTVDRVT
                     QGDGAVTDKPITVLVDGQTASAAERLAGSLETAGRAEVVGGSTYGKGLYQTSRVLPGG
                     CTVLVSTGESLDLQGAPIQGRGVRGTRAPAQGPPTDEPVPDR"
     gene            complement(4628..5338)
                     /locus_tag="VFD06_06040"
     CDS             complement(4628..5338)
                     /locus_tag="VFD06_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN03131.1"
                     /translation="MSGSGPSVLLVEDDPQIRRFIRATLPSHGLQLIEAATGREALSQ
                     AAVRTPDVVLLDLGLPDLDGLEVIRRLREWSAIPIIVLSARGLERDKVTALDAGADDY
                     LTKPFGVDELLARLRVALRHRAASGSGATTFATGDLSVDLAGRIVRLAAREIRLTPTE
                     FKLLAVLVRHAGKVVTHRQLLVEVWGPGSAGNTHYLRVQMHGLRHKLEAIPARPRYLI
                     TEPGVGYRLRDEPAASPA"
BASE COUNT          777 a         2042 c         2004 g          648 t
ORIGIN      
        1 agccggccgg gaacgccgga gccgcgccgc cgggggaggc cacctcggcc gacttcacgg
       61 tctgggtcga ggactgcgtc gcgggcgggc cggacgacgg cgcggccact gccggtggga
      121 cggtcgggcc gggagcggcg cccccgccgg agactcccgc accgggcgcg gcggcgccgg
      181 cggcggccgc cacgctgacg acgcaatgcg gccgctcgac gtggaaatcg atcgggccgc
      241 ttccccaacg cacgagggcc cacggatcgc cgctgatctc gaccctctcg accgacgatc
      301 cggcgcgcag gccgaaggcc ccgtccgcct cgagccgcag cccctcgagg atgaccacga
      361 ccggcgcgaa tccggcggtg gatcgcgcga tcgacaccgg cgcaccgagc gcggcggcgg
      421 ccttctcgac cgcctcctgg cgggcgtgct ccgccaccag gcgcgccagc cacgggcggg
      481 cgaccagcgc cgccagggcg agcgcgccgg ccgcgacggc gcaggtcaga agcaaacgtc
      541 cccgacggct catagaagca gaagaggata tcgcgccgcc gggttgggag ccctagcgac
      601 ggcggccgtg ctcgcgccag acctcggaga gggcgtgcca cgcctccacc cgccggcgca
      661 ccacgatcat catgaacgcc tcgtacgccg accagccgag gtagatgagg gtgaccggca
      721 ggtgcagccc ggggacgagg aactgagcga accagaggag gaagatcccc cccgcctcga
      781 cgatgtggaa cgacatgttc atgaggagga gcatcccgag cagcgactgc gagatggtca
      841 ggaggatctc cgcccgctgg tggtcgtcga acggcacgaa ggtcggggtc ccgacgctca
      901 tgctgtagac gaccgggatc atcgccgcca gcatcgtcca ctggttgatg ttcgccgaga
      961 ccatgttcat gagcgccatc gccgccttcc ccggccgccg cgcccagtac atggcggaga
     1021 gcttctcggg gaactccgac aggaacggcg ccacccactg gatgaagagg tactcggaca
     1081 ccccgagcgt gacggcgaag tgcagcatgc tctcgaggaa gggatgcgcc gaataggcga
     1141 gcaggagccc gccaccgacg aagagcccga ggatggcgaa cccctgggcc cgcttcggca
     1201 gccgcatgat gcggcgcggg accgcctcca gatcgtcgac gtgctcctcc tcccgcggcg
     1261 ggatcttccg cagcacccag aggtacagga agtagatcgc caggaggaga ccggcgtcca
     1321 cgagggtcag cgacgccttc agccagatga acaggaagta caggatcggc aggccgagcg
     1381 cgaacacctc gatggagttc tcgcggtcga gggtgatctg acgcagcagc cgcttctcgc
     1441 gccgccaggc tccgaacgcc gcgaccgaga agatgagcgg ccagcccagc ccgacgagca
     1501 ggcgcagcga gccggtgaag ttcgcggtca tgaagtggat ctgctgcgac caggcgatca
     1561 ccgcctcgac ggcgaattcc ggcagcgcct gcagccacgc gaggatcgcc agggccatgc
     1621 cgcgggacac ccagaactgc gcgcactccg ccccccacgc gatcatgaag gcggagaaga
     1681 ccaccgacgg catcggccac aggtggccga gcagcccctt gctcgctgcc gcctcgcgca
     1741 cccccggggc gatggcggcg agcgggagga agaagcgtcg tcgggggcgg ggatccctgg
     1801 gaggggtcgg cgtcatcggc cggccatctt acaggagcac gcccctcatc ggtcggggac
     1861 cggctcgtcc gtgggcggcc cctgcgccgg ggcgcgcgtg ccgcgcacgc cgcgcccctg
     1921 gatcggcgcc ccctgcaggt ccaagctctc ccccgtggag acgagcaccg tgcacccgcc
     1981 gggcagcacc cgcgatgtct ggtagagccc cttgccatag gtcgatccgc cgacgacctc
     2041 ggcgcgcccg gccgtctcga gactcccggc gagccgctcg gcggcgctcg cggtctgacc
     2101 gtccacgagc accgtgatcg gcttgtccgt gacggcacca tccccctgcg tcacccgatc
     2161 gaccgtcccc tccggcccgc tctcggatcc gacctcgacc ccgggaccga tgagcaggtc
     2221 ggccacctcg tccgccgatc gggtcagccc gccggcgttg ccgcgcaggt cgaggacgag
     2281 gccgctcttg tcgccgagcg ccgcgagcgc gcgcctcagc tcgcgcaccg cgttcggatc
     2341 ggtgaagttg gcgatctgca cgtagccgag gttgcccggc agctcctcgg tcgtgacggt
     2401 ggcgccgccg gcgcggttcc gccccagggc gtcggtctcc ctgggtccct ggtgccgcgt
     2461 cagccacacg gcggaggtct cctccgggtc acggagccgc gtgtacggat cgccgagcgt
     2521 ggcgagcaga agcgcgatgg cgcggagcgc ggcggagccc ggggcgaccg ccagctcgcg
     2581 gtggcgggcg cgcgcggcgt tccagtcctg gccgtgaaag gtgcgatcgc ggaacgagtc
     2641 gcgcgcgaac gcccacgcct cgaggtactg cagatccggc agcgccggca gcatcccgcc
     2701 gagcgcccgg cgtgcctcga aggtccgtcc gagcgacgcc agcaccgccg ggtactggct
     2761 gcgcgccagg tggaagttgt cggccggatc ggcggcgagg gcgcgggcgt agaagcgcgc
     2821 cgccccctcg gcgtcgtcct gatcggcccg cgccgcgccc aggagcgaca ggagcggcgc
     2881 cgcctccgga tgggactcga ggccgcgccg cgcggcctgc tcggactccg ccgcccgccg
     2941 caacctgaga tggaggagcg cgagatcgac gtgcggcgcc agccgcttcg ggtccagcgc
     3001 gatcgccttc tccaggctgg cgatcgccgc cgatcgatcg cggccggccc cctgctgagt
     3061 ctcggccagc gccgatcgcg gcacggcgag gttcggtcgg gcggcgacga cgcgatcgag
     3121 gatggcgcgc gcctcgccgg gatgtcccgc caggagatga cagcgcgcca ggaccaggag
     3181 cgacggcgtc gggtccgccg ccgcagcggc gaggtccttc tgcgcccgcg cggccaggtg
     3241 cgccagcatg tcgggacggc gtccgagcag atcggtgaac acgtcgagcg tccacggttc
     3301 ggtgctgtcg atccgcaggg ccgcttcgag ggcgatctcg acgtcggtcc acgcgccggc
     3361 gtccgccagc aggagggccc ggagccgcag gccggagatc ccggcggggg cggcggcgag
     3421 gacccgtccc cgcgcgtcgg cgcccgcccg ttcgacgaag gcggcgagcg cccgcgtcgc
     3481 gtacagatcg tcggggtgcg ccgccacgag gtcccgccag gcgcgctcct cggcgtcggc
     3541 gtcgccgcgc cgccgcgcca gggccgcctg atggtagagg gcgatcggat cgccgggcgc
     3601 cgcgcgcagc agcttcgcga actcggcgcc ggcccccgcc tcgtcccccg ccgcctcgag
     3661 cgcgagcgcg agaccgcgcc gcaggccgtt gtcgccgggc gcccgcttca gcgcggcccg
     3721 ataagcttcg gccgctccgg gggcgtcgcc ggcgatggcg cgcagccgcc cgatctcccc
     3781 ccacacctcc gccccggccc ggccggagcc ggacgtcttc gccgccgccc cgagcgccgc
     3841 gaggagcgcg cgcacctcct gcagctcgtc gcgacgcgct cgcgccggcg cgtcgtccgg
     3901 cgccagcgcg aggaaccgat cccacgccgc gacggcgcca tcgagatcgc cgcgctcttc
     3961 ggcgaggcgc gccagcccgg cctgcgccgg ggcgttcttc gggtcggtcg cgagcccctc
     4021 ccggaaactc tcctccgcgt ccttcgtctt cccctcgcgt cgccgcgtct cgccgagggt
     4081 caccaggagg tcggcgcgct cggccccctg caccttcttc agggccggaa ggagcagcgc
     4141 ttccgcgccg tcgaagtcgt actccgcccg cttgagaatc gcctggccgg tgtacccctc
     4201 ggcgcggccc ggggcgagcg tcgtcgccgc ctcgtaggcg gccgccgcct cggagacgcg
     4261 gccgacgcgc cgcagggtgt cggcgatgag aatgcggatc tcgtagcgtt tcggatcgag
     4321 gccggaggcg gagcgatagg cgtcgagggc cgcctgcagc cgcccttcgc ggcgcgcctt
     4381 gttcccttcc tgcacgagcc cgttcaccgc ggcggtgacg gcggcgttct gccgcgcctc
     4441 gaccgcggcc ggatcggggg ccgggtcctc ggcggcggcc ggaaggacga tcgcggcgag
     4501 gagcggcagc gacacgagaa gggcgcggga gcggcgattc acgaggggcg ccaaggtacg
     4561 gcgacaccgc cccgaagtca aacggaagcg acccgggtcg gcgaagaccg ggcccgggga
     4621 acgcccgtca ggcggggctc gcggccggct cgtcccgcag gcgatagccg acgccgggct
     4681 cggtgatgag gtagcgcggg cgcgccggga tcgcttcgag cttgtgacgc agtccgtgca
     4741 tctgcacccg gaggtagtgg gtgttccccg ccgatccggg cccccacacc tcgaccagca
     4801 gctgcctgtg cgtcaccacc ttccccgcgt gccgcacgag gacggcgagg agcttgaatt
     4861 cggtgggagt gagacggatc tcgcgggcgg cgaggcgcac gatgcggccg gccaggtcga
     4921 cgctcagatc gccggtcgcg aacgtcgtcg cccccgagcc ggaggcggcg cggtggcgca
     4981 atgcgacccg aagccgcgcc agcagctcgt ccacgccgaa cggcttcgtc aggtagtcgt
     5041 cggccccggc gtcgagcgcc gtcaccttgt cccgctcgag tccacgggcg gacagcacga
     5101 tgatcgggat cgccgaccac tcgcgcagcc gacggatcac ctcgaggccg tcgaggtccg
     5161 gaagcccgag gtcgaggagc acgacgtccg gtgtgcgcac cgccgcctgc gacagcgcct
     5221 cgcggccggt ggccgcctcg atcagctgca ggccgtgcga cggcagcgtg gcgcggatga
     5281 agcggcggat ctgcgggtcg tcctcgacga ggagcaccga cggaccggac ccgctcacgc
     5341 ccagcctccc gcctcgagcg cgttcacgct ccgcctcccg ccgcttcgac tgcgcccgcc
     5401 tcgccccctt ccgccgggat cacggccgga ggctcgcctc ccagcggcag cgtgaaacgg
     5461 aaggcggctc c
//
LOCUS       DASRIQ010000244         5007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2176963, whole genome shotgun sequence.
ACCESSION   DASRIQ010000244 DASRIQ010000000
VERSION     DASRIQ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176963"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VFD06_06045"
     CDS             <1..1447
                     /locus_tag="VFD06_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03132.1"
                     /translation="WVQTRFHLDGYLGRRVRVRWIAESWAFDEHSSSYYQIGGTWAAT
                     QQEDGWWLDDIQVTGVVTQQLSPTLDTTPRTGTCPADPCNATVGDRGTHAVIEVTDAA
                     GAPIDGVTRIPTTGERVRLGAGASTFPGGCSNGHAEYQFLRDGLVAQAWSANPFFTDS
                     PERTTRFTALVRCTSDPGCTSVVGASIDLPVRSGEGGDVVFGARGALLDPGAGVVYYR
                     GACSAGIVGAPCNGAIDCGAGGACAPTAATADDVTVLRLWSASDDGLDVIRGNVPAGP
                     APKGTLSGSFWTLPGLSGPCFLSNLPAMAAATGFNHTSGPRTQAQDPNPPLGGVVYYQ
                     AAANGPGGANLDAYGCPSPSICSNPGWCDLGTNAGGPCTTGAECPGGGTCVLATTFCS
                     TDAGTAGQGGCGHHLTCAGGSNSGKLCQSVLHCPGGGTCPAATATQQATEGQVCLTLT
                     GAPLAPAPYGNCPPAGHPKRIVSRSDGLACP"
     gene            2237..>5007
                     /locus_tag="VFD06_06050"
     CDS             2237..>5007
                     /locus_tag="VFD06_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN03133.1"
                     /translation="MTLRRLRSPLWLLVLLLLPGALLFGSRPQPLPEGLEEDPVERAM
                     QLRGARAAERFQLPDPVAPPAEFSPFGQGMPFVILEGEAARGMLLTSQGHVHWDAGRD
                     AVAARIPAGLRAPEAELRRSERGTLRPGLNYLRLSEAETASRGSDAVQAEVARHARIL
                     GWLPERTVVVWVEAREMEALSRVPGVDASRALEPYHKISPALGALPRLSRREAENPDL
                     LARVTFVPGRGGERTIEVVRRMPGVSEVVPDATSDDGLQLRVHFDRVVDLARRDEVLS
                     IEPVLDFVLANAENVPTVQAGSAEDARLARPFDDAGVDGGGIDTNGDGMRLNDGSDLV
                     PPQLVAITDNGISLDTPSFAQTMSATILAGIPIGPVHRKIHVIQNVTDSGTTCDAPLS
                     GAGTHGNVVASVIAAYPSFLGAYLTRNGIGGPTSPRNENLDGIARGARIIVQDAGTTS
                     QCTINSLVERGGNVSPGPLLDRLNAALASGGSQVHLSIFPFGAPANFSTIQFLASNGT
                     YPSESAQIDTFLYNNRDYMVFVPAGNYGGLLGTGRLGLMLRIIPDLFNGTAADENPSF
                     PAPIQIPPPATAKNIVTVGAGTADCFTFFGSTDCEQTIANFTSRGPATPQSLRMAPIL
                     TAPAFDLIGTPFAASLAVFRSNDNDNLGPIDAQLDEGNFGTSFAAASLTGAGALIRDY
                     FAQGFYPTGSRGAAADRMPGISGAVVKAALVASADFNEGGIATQGQDNNERNLRRTRC
                     LDLGTVSGTAVDIMCNSEQGYGRAVLTNVLPLANWSDQFVLHPTSLLAREYPAAGLLV
                     WDRLATGEPFINNTTQTSVTHTFRVASPNIITKVAPAPDAGAVAVAPGQLRIAVSWAD
                     PPSLAGSGGPLINDLDLVVESPGPDGNLATAVDNIFYDGNRYDGGRNNASFDQWSLGR
                     TSTTPVEKH"
BASE COUNT          735 a         1847 c         1717 g          708 t
ORIGIN      
        1 gtgggtccag acgcgcttcc atctggacgg ctatctcggc cggcgcgtcc gcgtccgctg
       61 gatcgccgag tcgtgggcct tcgacgaaca cagctcctcg tactaccaga tcggcggcac
      121 ctgggcggcg acgcagcagg aggacgggtg gtggctcgac gacatccagg tgaccggcgt
      181 cgtgacgcag cagctctcgc ccacgctcga cacgactccg cgcaccggga cctgcccggc
      241 cgatccgtgc aacgccacgg tcggcgatcg ggggacgcat gcggtgatcg aagtgaccga
      301 tgccgccggg gcgccgatcg acggtgtcac gcgcatcccg acgacgggcg agcgcgtccg
      361 cctcggcgcc ggcgcctcga ccttccccgg cggctgctcg aacgggcacg cggagtacca
      421 gttcctgcgc gacgggctcg tggcgcaggc ctggagcgcc aacccgttct tcaccgacag
      481 cccggagcgc accacgcgct tcacggcgct cgtgcgctgc accagcgatc ccgggtgcac
      541 gagtgtcgtc ggcgcatcga tcgacctgcc ggtccgatcc ggcgaggggg gagacgtggt
      601 gttcggggcg cgcggcgcgc tgctggaccc cggcgccggc gtcgtgtact accgcggcgc
      661 ctgcagcgcc gggatcgtcg gggcgccgtg caacggggcg atcgactgcg gggcgggcgg
      721 cgcctgcgcc ccgaccgcgg cgacggccga cgacgtcacg gtgctgcggc tctggtcggc
      781 ctcggacgac ggactcgatg tcatccgggg gaacgtcccg gcgggcccgg ccccgaaggg
      841 gacgctgtcc gggtcgttct ggacgcttcc gggactctcc ggtccctgct tcctgtcgaa
      901 ccttccggcg atggccgccg ccaccgggtt caaccacacc tcgggaccca ggacccaggc
      961 gcaggacccc aacccacccc tgggaggcgt cgtctattac caggcggcgg cgaacggtcc
     1021 gggcggggcc aacctggacg cctacggctg tcccagcccg tcgatctgca gcaacccggg
     1081 ctggtgcgac ctgggaacca acgccggtgg accgtgcacg accggcgccg agtgccccgg
     1141 aggcgggacg tgcgtcctcg cgacgacctt ctgctcgacc gacgccggaa cggcgggcca
     1201 ggggggctgc ggccaccacc tgacctgcgc aggggggagc aactccggga agctctgcca
     1261 gtcggtgctg cattgtcccg gagggggcac gtgcccggcg gcgaccgcga cgcagcaggc
     1321 gaccgaagga caggtctgcc tgacgctgac cggcgcgccg ctggcgccgg ccccctacgg
     1381 caactgtccg ccggccggcc acccgaagcg gatcgtcagc cgctccgacg gtctggcctg
     1441 cccgtgagga actgccgtcc acccggcgcc agaccggcgg cgccgggaga aatgtcgttc
     1501 caacggaacc tttgctccat gtggcacgac caaggatcat ccgggttttc cgtgattaac
     1561 cggaatcgcc cgcgctgtat atgctgtttt aacattcgcc gatagctcgc tctgtaccgg
     1621 aaaagacatt ctgctgtccg cctccagggg gatcggaagg tcgaggttcc ggccgggagg
     1681 aatgtcccat gacgcgacgt gcaacgcgtt cgctcctctg gctttccgtc gtgctcctgc
     1741 ttcccggcgc ggtcctgttc ggcggcgggc tccagccgct tctggaaggg gagggcgaag
     1801 acccggtcga gcgggcgatg caggccggcc tcgggcgggc ggcggagcgt ttccggctcc
     1861 cggaaccgac catgccgccg cccggcggcg cagcaggcga ccgagggaca ggtctgcctg
     1921 acgctgaccg gcgcgccgct ggcgccggcc ccttacggca actgtcctcc ggccgggcac
     1981 ccgaagcgga tcgtcagccg ctccgggggt ctggcctgcc cgtaagggac cgtccccccg
     2041 gcgccggacc ggcggtgccg gggggaacgt tcggagccac gcgaccgctt ggcgacgagg
     2101 gtcacgttga ccggggtggt tcgacgcggt atatggtttt gcaacatcgc tcgatcgctc
     2161 tgaatgcgcc ggaaccgacc tgccgcagtc gccccgaggg gcaacggggg tcgtgttccg
     2221 gcgaggaggt gcatccatga cgctccgacg cctgcgatcg cccctctggc tcctcgtcct
     2281 cctcctgctc ccgggggcgc tcctcttcgg cagccggccc cagccgctgc cggaaggcct
     2341 ggaagaggac ccggtcgagc gggcgatgca gctccgcggc gcgcgcgcgg cggagagatt
     2401 ccagctcccc gatccggtcg cccccccggc ggagttctcc ccctttggac aggggatgcc
     2461 gttcgtcatc ctcgaggggg aggcggcccg cggcatgctc ctcaccagcc aggggcacgt
     2521 ccactgggac gccgggcggg acgccgtcgc cgcccgcatc ccggcggggt tgcgggcccc
     2581 agaggcggag ctgcggcggt cggagcgcgg cacgctccgt ccgggcctca actacctgcg
     2641 actctcggaa gcggagacgg cctcccgcgg ttcggacgcc gtgcaggcgg aggtcgcccg
     2701 gcacgcccgc atcctcggct ggctcccgga gcggaccgtc gtcgtctggg tcgaagcgag
     2761 ggagatggag gcgctgtcgc gcgtccccgg cgtcgatgcc agccgggcgc tggagccgta
     2821 ccacaagatc tccccggctc tcggcgcgct gccgcggctc agccggcgcg aggcggaaaa
     2881 ccccgacctc ctggcgcgag tgaccttcgt tcccgggcgc ggcggcgaaa ggacgatcga
     2941 agtggtccgg agaatgccgg gtgtgagcga ggtcgtcccc gacgcgacca gcgacgacgg
     3001 cctgcagttg cgggtgcact tcgatcgggt cgtcgatctc gcgcgccgcg acgaggttct
     3061 gtcgatcgag ccggtgctcg acttcgtcct ggcgaacgcc gagaacgttc caaccgtgca
     3121 ggccgggtcg gccgaggacg cccgcctggc gcgcccgttc gacgacgccg gggtggacgg
     3181 cggcggcatc gacaccaacg gcgacggcat gcgcttgaac gacggaagcg acctcgtccc
     3241 gccgcagctc gtcgccatca ccgacaacgg catcagcctc gacacgccgt cgttcgcgca
     3301 gacgatgtcg gcgacgatcc tggccggcat cccgatcggt ccggtgcacc ggaagatcca
     3361 cgtcatccag aacgtcaccg acagcggcac cacctgcgac gcgccgctgt ccggagcggg
     3421 aacgcacggc aacgtggtcg ccagcgtcat cgccgcgtac ccgagtttcc tcggcgccta
     3481 cctgacccgc aacggcatcg gcgggccgac ctcgccgcgc aacgagaacc tcgacggcat
     3541 cgcccgcggc gcccgcatca tcgtccagga cgccggcacg acctcgcagt gcaccatcaa
     3601 ctccctggtg gagcgaggcg ggaacgtcag ccccgggccg ctcctcgacc gcctcaacgc
     3661 cgccctcgcg agtgggggca gccaggtcca cctcagcatc ttcccgttcg gcgcgccggc
     3721 gaatttctcg acgatccagt tcctggccag caacggcacc tatccgagcg agtcggcgca
     3781 gatcgacacc ttcctttaca acaaccggga ctacatggtg ttcgtgccgg ccgggaacta
     3841 cggcggcctc ctcggaaccg gccggctcgg gctgatgctc cggatcatcc ccgatctgtt
     3901 caacggcacc gcggccgatg agaacccgag cttcccggcg ccgatccaga tcccgccgcc
     3961 ggcgacggcg aagaacatcg tcaccgtcgg ggcgggaacc gccgactgct tcaccttctt
     4021 cgggtcgacc gactgcgagc agacgatcgc caacttcacc tcgcgcggtc cggcgacgcc
     4081 gcagtcgctg cggatggcgc cgatcctcac cgcccccgcc ttcgatctca tcggcacccc
     4141 gttcgccgca agcctcgcgg tcttccgcag caacgacaat gacaatttgg ggcccatcga
     4201 cgcccagctg gacgaaggga acttcgggac ctccttcgcg gccgcgtccc tgaccggggc
     4261 cggagcgctc atccgcgact atttcgccca ggggttctac ccgaccggca gccgcggcgc
     4321 cgctgccgat cggatgcccg gcatctccgg ggcggtggtc aaggccgcgc tcgtcgcctc
     4381 ggccgacttc aacgaaggcg gcatcgccac gcaggggcag gacaacaacg agcgcaacct
     4441 gcgccgcacc cgctgcctcg atctcggcac cgtctcggga accgcggtcg acatcatgtg
     4501 caacagcgag cagggatacg gccgggcggt gctcaccaat gtcctgccgc tggcgaactg
     4561 gtcggatcag ttcgtcctgc accccacctc cctcctggcg cgcgagtacc cggccgccgg
     4621 gctgctggtc tgggaccggc tggccacggg cgagccgttc atcaacaaca cgacccagac
     4681 gtcggtgacg cacaccttcc gcgtcgcgtc gccgaacatc atcaccaagg tggccccggc
     4741 gcctgacgcc ggcgcggtcg ccgtcgcccc cggacagttg cgcattgcgg tctcgtgggc
     4801 ggatccgccc agcctcgccg gctccggcgg tccgctgatc aatgacctcg atctcgtcgt
     4861 cgagagcccc ggtccggacg gcaacctcgc cacggccgtg gacaacatct tctacgacgg
     4921 gaaccgctat gacggcggcc gcaacaacgc gagcttcgac cagtggtccc tgggccgcac
     4981 ctccaccacc ccggtggaga agcacga
//
LOCUS       DASRIQ010000245         8118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_873610, whole genome shotgun sequence.
ACCESSION   DASRIQ010000245 DASRIQ010000000
VERSION     DASRIQ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873610"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VFD06_06055"
     CDS             <1..1087
                     /locus_tag="VFD06_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZN03134.1"
                     /translation="GRESAETDGLPFPLSARGTVYFHRGGGALYHLMRTLRFGPAATV
                     LVPDYHGGNEIAALRAAGARLIFYRVDRFMQPDLDQIERLSPGARCLYVIHYLGWPQP
                     MHAIRDLCRERGLFLIEDCALSFLSTTDEGPLGRLGDFSIFCLYKTLPVPNGGLLVQN
                     TLRLDGIEAAPQKHAGLSTLFGRTADLLIRRVHGRSRRTGRALVAMRDGAGRLARRLP
                     SRRQPVAGGGFDAGDTHAGIAWLSRALLGRFDYNAIVQRRRRNFRRMADRLAGRVEML
                     RTDLPDGVCPLFLPILVSDKEAAARALKDRGVEAVEFWNQGDLLGSLAEGADARHLRR
                     HLLEIPIHQDLGDEAIDFASDQVLAVV"
     gene            1084..2256
                     /locus_tag="VFD06_06060"
     CDS             1084..2256
                     /locus_tag="VFD06_06060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN03135.1"
                     /translation="MTRVERIHDADGFRRLRPEWTELLAASPADTVFLTWEWLHTWWT
                     HFAGRRRLALMAVREGQDLIALAPLALRPPQSSRLSFAWRAEFLGSGTAGSDYLDIIV
                     RDGQEEPALEALAGPLERAGAALDLTQTRRGASIADRLGERLRDRGWWLRRSATNACP
                     FIPLVGHTWSSYLTSLGSSHRANLNRRLRALARTCDVSFDSARTEEDRRLFLARLVEL
                     HNARRRTLDGSEAFDRPERIAFHDRFSALALERGWLRLFLLRLDGLPVASLYGLRYRD
                     VFSFYQSGFDPAYARQSVGLVTMGWSIRAAIDEGASEFDFLHGEESYKSLWTRKRRAL
                     GRLEIYPPRPAGAFARRMDDAGRGARRLARALLPEGTADRIARGLQAGAEGRPDAS"
     gene            2246..3382
                     /locus_tag="VFD06_06065"
     CDS             2246..3382
                     /locus_tag="VFD06_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN03136.1"
                     /translation="MLRDLVKSGMAHALHASGGGRLYGSLRGARRSPVILGYHRVVDS
                     FDAAATTAIPALLISTGTLERQLAWLQRDRKVVPLDEIGERVFAGQPVDHLAAVTFDD
                     GYRDVYEQAFPILKRMGIPAAVFVVAGTVGTAQMLNHDRLYGLLRIGYQRWTSPSREL
                     ALLLDGCGVAPLHADALAAARGSAYTATRLLLAFYPRADLLALIGALEARFGVEGENG
                     GLPMSWDMLREMRAAGITIGSHTMSHALLDLEGRRTVRDELADSRRTLEAHLGARIDH
                     FAYPDGRFDAATIEEVAREYRYAYTICGHRDPRFPRHTLSRRVLWERSCSSGSESMVA
                     SILACQVNGLFDPMSPCRRPHGASITGRSIPPGQQRRDWKGALA"
     gene            3379..4872
                     /locus_tag="VFD06_06070"
     CDS             3379..4872
                     /locus_tag="VFD06_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZN03137.1"
                     /translation="MNDPRRRWWPRLRPGPEVVLILGRGIGCAAAFLIPVALARVFDP
                     TDFGTYKQLFLIFATLYYIAQAGMAESLFYFVPRDRAGGGRYAVNSILALALMGLLTF
                     AGLLLLAPRVASFMANPALVEGIPWIGLFLLLMLASAGWEILLMARGRYRSAAFAYAG
                     SDVVRALALTLPGVLLGSVTAVIWGAIAFAVLRIGATIATLVREFPGALAPDGAFFGR
                     QLAYALPFGAAVVVEILQANLHQYVVSAKVEPAVFAIYAVGCLQIPLIDLVAGPSGNV
                     MMVRMSEKGVGPAGMLALWHGTIRKLAFVFLPLAAFLMVSARDLIEALFTSRYAASAP
                     IFVAWSATVVLASLPTDCVLRVHAGTRYLLAVNVARLLVVIASIGLLMRGFGLVGAVL
                     ATFLGQVVAKGAMLWRIGRLLHAGPVRLLPWGSLLSSAAVAIGAALPVLFAGPFLGAS
                     PMVRLAADTVIFGVAGLLLFLLLGGRPATFPRLPLRSSESCAVSQAS"
     gene            4848..6737
                     /gene="asnB"
                     /locus_tag="VFD06_06075"
     CDS             4848..6737
                     /gene="asnB"
                     /locus_tag="VFD06_06075"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN03138.1"
                     /translation="MCGIAGIVTRDGAPVAKRLVAGMCGALRHRGPDDEGLVTGSGAC
                     LGMRRLAIIDPAHGHQPMSNEDGSVQVVLNGEIYNYRELRRRLIRNGHQVRTHSDTET
                     IAHLYEEHGAGLVEHLRGMFAFAVWDERRRTLLLARDRLGVKPLYYLEDGRRLAFASE
                     LKALLRLPGVEPEIDWGAAAHLFAYLTTPRTKSIVRGIRKLPPGHVLLATPGQPSWIA
                     PYWEVRFEPDRSRRPEERVEELKALLDESIRLRLVSDVPVGAFLSGGLDSSAVVAAMA
                     RQVSGPIQTFTIGFNEPEYDERPAARLVAGAFGTEHHELLLDPDLTSEIETIAWHLDE
                     PFGDSSAIPTYMVSKLASRHVKVVLTGDGGDEVFAGYDRYVVEAQRRRRRRVPAFLRG
                     PLGRLAAALPPQTRGRNFLRSLSLTGPERYLDAATLLRQDALERLLRPEAHAAMSRSD
                     AETDRGGLRERWGSDWLGAAQWHDLTSSLPLDILTKVDRMSMAHSVEAREPLLDHRLI
                     EWAATLPPEDRLRGTTTKWIFRQAIRDMVPQAILDRPKRGFAVPLGRWFRGRLDGTLR
                     DLLLSRRSRERGVFEPREIERLIAAHERGRPLDLELWTLMSFELWCRAFQDAGPRTLR
                     EVSCA"
     gene            6730..7857
                     /locus_tag="VFD06_06080"
     CDS             6730..7857
                     /locus_tag="VFD06_06080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN03139.1"
                     /translation="MPEAILTAARPLRIVMVAPGRGATGGQETQASTLEDRLRAEGHR
                     VRRIPIDPAPPHALRWARDVRYARTILNEALYVRSLAALRRADVVHVFSASYWSFLLA
                     PLPALLAGRLFGRRVILHYHSGEAEEHLGRWGMLVHPWLRLADAVVVPSLYLREVFAV
                     HGYHTRHIPNIIETDRFRFRPRMPLQPRFVSTRNLEPGYRVDVTIRAFALLRERYPDA
                     TLLVAGRGSEEDRLRALASEVGPQGIRFLGALDRSHMPQLLERGDILLNASVIDNQPV
                     SILEAFAAGLLVVTTVPGDLRHMVRDGETGRIVPPANPKAMAQAAAALLEDPERAARI
                     AARARREAGRHSWGAVRDAWMKAYGCPVAAAGSAGMAGAAA"
     gene            7857..>8118
                     /locus_tag="VFD06_06085"
     CDS             7857..>8118
                     /locus_tag="VFD06_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03140.1"
                     /translation="MRIERWTSIGPREIAHRGRQEVSKWIDRIVPPAARMPLQEAAPP
                     AAAPAAPPFFAGLDSPLVREMFEGRLGVARAQLLAAARRFAEG"
BASE COUNT          962 a         3015 c         2911 g         1230 t
ORIGIN      
        1 ggggcgcgag agcgccgaga cggacggcct gccgttcccg ctctcggcgc gcggcacggt
       61 ctatttccac cgcggcggcg gcgccctcta ccacctgatg cgcacgctgc gcttcggacc
      121 ggcggcgacg gtgctggtgc cggactacca cggcggcaac gagatcgcgg cgctccgcgc
      181 cgcgggggcg cggctcatct tctaccgcgt cgatcgcttc atgcagccgg acctcgatca
      241 gatcgagcgc ctgagcccgg gcgcgcgctg cctctacgtc atccactacc tcggctggcc
      301 gcagccgatg cacgccatcc gcgacctgtg ccgggagcgc ggcctgttcc tcatcgagga
      361 ctgcgccctg tcgttcctca gcacgaccga cgaggggccg ctcggccgcc tcggcgactt
      421 ctccatcttc tgcctctaca agacgctgcc ggtgccgaac ggcgggctcc tggtgcagaa
      481 caccctgcgg ctcgacggca tcgaggcggc gccgcagaag cacgccggcc tctcgaccct
      541 gttcgggcgc accgccgacc tgctgatccg ccgcgtgcac gggcgctcgc gccgcaccgg
      601 ccgcgccctc gtcgcgatgc gcgacggcgc cggccgcctg gcgcgccgcc tgcccagccg
      661 ccggcagccg gtcgccgggg gcggtttcga cgccggcgac acccacgccg gcatcgcctg
      721 gctgtcgcgg gcgctcctgg gccgcttcga ctacaacgcc atcgtccagc gccgccgccg
      781 gaatttccgg cgcatggcgg atcgtctcgc gggacgggtc gagatgctgc gcaccgacct
      841 gccggacggg gtctgcccgc tgttcctgcc gatcctcgtg tccgacaaag aggcggcggc
      901 gcgcgcgctg aaggatcgcg gcgtcgaggc ggtggagttc tggaaccagg gggatctcct
      961 cggctcgctc gccgaagggg cggacgcgcg gcacctgcgc cggcacctgc tcgagatccc
     1021 gatccatcaa gacctggggg acgaggcgat cgacttcgcc tccgaccagg tgctggcggt
     1081 cgtatgacgc gggtcgagag gatccatgac gccgacgggt tccgccgctt gcggccggag
     1141 tggaccgagc tgctcgccgc cagccccgcg gacacggtct tcctgacctg ggaatggctc
     1201 cacacctggt ggacccattt cgccggacgg cgccgcctcg ccctcatggc ggtgcgcgag
     1261 gggcaggacc tcatcgcgct cgcccccctg gcgctgcggc cgccgcagtc gtcccgcctg
     1321 tcgttcgcct ggcgcgccga gttcctcggg agcggcacgg ccggctcgga ctacctcgac
     1381 atcatcgtgc gggacggcca ggaagagccg gccctcgagg ccctggcggg cccgctggag
     1441 cgggcgggcg cggcgctcga cctgacccag acgcgccgcg gcgcctcgat cgccgaccgg
     1501 ctgggggagc ggctgcggga ccgcggctgg tggctcagga ggagcgccac caacgcctgt
     1561 cccttcatcc cgctcgtggg gcatacctgg agcagttacc tgacgtcact ggggtcgtcg
     1621 caccgcgcca acctcaaccg gcgactgcgc gccctggcgc ggacctgcga cgtgagcttc
     1681 gattcggcgc gcaccgagga ggatcggcgg ctcttcctcg cccggctcgt cgagctgcac
     1741 aacgcgcggc gccgcaccct ggacggctcg gaggcgttcg atcgccccga gcggatcgct
     1801 tttcacgacc gcttcagcgc gctcgccctc gagcgcggct ggctgcggct gttccttctg
     1861 aggctcgacg gcttgccggt ggcgtcgctg tacgggctgc gctaccggga cgtcttctcc
     1921 ttctaccagt ccggattcga tccggcctac gcgcgccaga gcgtggggct ggtgacgatg
     1981 ggatggtcga tccgggccgc gatcgacgag ggggcgtcgg agttcgactt cctgcacggc
     2041 gaggaatcct acaaatcact ctggacccgc aagcggcgcg ccctcggaag gctggagatc
     2101 tatccacctc gtcccgcggg ggcgttcgcc cgccggatgg acgacgcagg acgcggcgcc
     2161 cgccggctgg cgcgcgcgct cctgcccgag ggcaccgccg accgcatcgc gcgcggcctg
     2221 caggccggcg cggaggggag acccgatgct tcgtgacctg gtcaagtccg gaatggccca
     2281 tgccctgcac gcgagcgggg gcggccggct gtacggcagc ctgcgcggcg cccgccgctc
     2341 ccccgtcatc ctcgggtacc accgcgtggt cgactcgttc gacgccgccg cgacgaccgc
     2401 gatcccggcg ctgctgatct ccaccggcac cctggagcgg cagctggcgt ggctgcagcg
     2461 cgaccggaag gtggtgccgc tcgacgagat cggcgagcgc gtgttcgccg gacagccggt
     2521 ggaccacctc gccgccgtca ccttcgacga cggctaccgc gacgtctacg agcaggcctt
     2581 cccgatcctc aagcgcatgg ggatcccggc ggcggtgttc gtggtggcgg gaaccgtcgg
     2641 cacggcgcag atgctcaacc acgatcgcct gtacggtctg ctgcggatcg gctaccagcg
     2701 ctggacctcg ccgtcgcgcg agctggcgct cctgctcgac ggctgcggcg tcgcgccgct
     2761 gcacgccgac gcgctggccg cggcgcgcgg ctccgcctac accgcgacgc gactcctcct
     2821 ggccttctac ccgcgcgcgg acctgctggc cctcatcggc gccctcgagg cgcgcttcgg
     2881 ggtggagggc gaaaacggcg ggctgccgat gtcgtgggac atgctgcggg agatgcgggc
     2941 cgccgggatc accatcggct cgcacaccat gagccacgcg ctcctggatc tcgagggacg
     3001 gcggacggtg cgggacgagc tggcggactc gcgccgcaca ctcgaggcgc acctcggggc
     3061 gcgcatcgat cacttcgcct accccgacgg acgcttcgac gccgccacca tcgaggaggt
     3121 ggcgcgcgag taccgctatg cctacaccat ctgcgggcac cgcgatccgc gctttccgcg
     3181 ccacaccctg tcgcgccgcg tgctctggga gcgctcctgt tcctcgggga gcgagtcgat
     3241 ggtcgcctcg atcctcgcct gccaggtgaa cggcctgttc gatccgatgt ccccgtgccg
     3301 ccgccctcac ggcgcgtcga tcaccgggcg ctccatcccg cccggtcagc agcgcaggga
     3361 ctggaagggg gcgctggctt gaacgatccg cgccgccggt ggtggccgcg cctgcggccg
     3421 gggcccgagg tggtcctcat cctcgggcgc ggcatcggct gcgcggccgc cttcctcatc
     3481 ccggtggcgc tggcgcgggt cttcgacccg accgacttcg gaacctacaa gcagctgttc
     3541 ctgatcttcg cgacgctgta ctacatcgcc caggccggca tggcggagag cctcttctac
     3601 ttcgtgccgc gcgatcgggc cggcggcggg cgctatgcgg tgaactcgat cctggcgctc
     3661 gccctgatgg ggctcctgac cttcgccggg ctcctgctcc tggcgccgcg cgtcgcctcc
     3721 ttcatggcca acccggcgct cgtcgagggg atcccgtgga tcggcctctt cctcctcctg
     3781 atgctggcct cggccggctg ggagatcctc ctgatggcgc gcggccgcta ccgctcggcc
     3841 gccttcgcgt acgccggctc ggacgtcgtg cgcgccctgg cgctgacgct tccaggggtg
     3901 ctcctcggca gcgtcacggc ggtgatctgg ggggcgatcg ccttcgcggt gctgcggatc
     3961 ggggcgacga tcgcgacgct cgtccgggag ttcccgggcg cgctcgcgcc ggacggggcg
     4021 ttcttcggcc ggcagctggc ctacgcgctg ccgttcggcg ccgcggtggt cgtcgagatc
     4081 ctgcaggcga acctgcacca gtacgtggtg tcggcgaagg tcgagccggc ggtcttcgcg
     4141 atctatgccg tggggtgcct gcagatcccg ctcatcgatc tcgtcgccgg tccgtcgggg
     4201 aacgtcatga tggtccggat gtcggaaaag ggggtcggcc ccgccggcat gctggccctc
     4261 tggcacggca ccatccgcaa gctggcgttc gtcttcctgc cgctcgccgc ctttctcatg
     4321 gtgtcggcga gggatctcat cgaggcgctc ttcacgtcgc gctacgccgc gagcgcgccg
     4381 atcttcgtcg cctggagcgc cacggtcgtc ctcgcgtcgc tcccgaccga ctgcgtgctg
     4441 cgggtgcacg ccgggacgcg ctacctcctc gccgtcaacg tcgctcgcct gctcgtcgtg
     4501 atcgcatcga tcgggctcct catgcgcggg ttcggcctgg tgggcgcggt gctcgccacc
     4561 ttcctcggcc aggtggtggc gaagggggcg atgctctggc gcatcggccg gttgctgcac
     4621 gcgggaccgg tgcgcctgct gccgtggggg tcgctcctgt cgtcggccgc cgtcgcgatc
     4681 ggcgcggcgc tcccggtcct gttcgccggg ccgttcctgg gcgcatcgcc gatggtgcgt
     4741 ctcgccgccg acacggtcat cttcggagtc gcggggctcc tgctgttcct tctcctgggg
     4801 gggcggcccg ccacgttccc ccgtcttccc ctgaggtcct cggaatcatg tgcggtatcg
     4861 caggcatcgt gacgcgtgac ggcgccccgg tggccaagcg cctcgtggcc gggatgtgcg
     4921 gggcgctccg gcaccgcgga ccggacgacg aggggctcgt gaccggctcc ggcgcctgcc
     4981 tcggcatgcg ccggctcgcc atcatcgacc cggcgcacgg ccatcagccg atgagcaacg
     5041 aggacggctc ggtgcaggtg gtgctcaacg gcgagatcta caactatcgg gagctgcgcc
     5101 ggcgcctcat ccggaacggg caccaggtga ggacccacag cgacaccgag acgatcgcgc
     5161 acctctacga ggagcacggc gccgggctcg tcgagcacct gcgcggcatg ttcgcgttcg
     5221 cggtgtggga cgagcggcgc cgcacgctgc tcctggcgcg cgaccggctc ggcgtcaagc
     5281 cgctctacta cctcgaggac ggccgccgcc tggcgttcgc ctccgagctg aaggcgctcc
     5341 tgcgcctgcc gggggtcgag ccggagatcg actggggggc ggcggcgcac ctcttcgcgt
     5401 acctcacgac gccgcgcacc aagagcatcg tgcgcggcat ccgcaagctc ccccccgggc
     5461 acgtgctcct cgccacgccg ggccagccgt cctggatcgc tccctattgg gaggtgcgct
     5521 tcgagccgga tcgctcgcgc cggcccgagg agcgcgtcga ggagctgaag gcgctcctcg
     5581 acgagtccat tcgactgagg ctggtgagcg acgtcccggt cggggcgttc ctgtcgggcg
     5641 gtctcgacag cagcgcggtg gtggcggcga tggcgcggca ggtgtccggc ccgatccaga
     5701 ccttcaccat cggcttcaac gagccggagt acgacgagcg tccggcggcg cgcctcgtgg
     5761 ccggcgcctt cggcaccgag caccacgagc tgctcctcga ccccgacctg acgagcgaga
     5821 tcgagacgat cgcctggcac ctcgacgagc cgttcggcga ctcctcggcg atccccacct
     5881 acatggtcag caagctcgcg tcgcgtcacg tcaaggtggt gctcaccggc gacggcgggg
     5941 acgaggtgtt cgcgggctac gaccggtacg tcgtcgaggc gcagaggcgc cgccggcgcc
     6001 gggtcccggc gttcctgcgc gggccgctcg ggcggctggc ggcggcgctg ccgccccaga
     6061 cgcgcggacg gaacttcctg cgcagcctgt ccctcaccgg cccggagcgc tacctcgacg
     6121 ccgccacgct cctgaggcag gacgcgctcg agaggctcct ccggccggag gcgcacgcgg
     6181 cgatgtcccg gtccgatgcc gagaccgatc ggggcgggct ccgcgagcgc tgggggagcg
     6241 actggctggg ggcggcgcag tggcacgacc tgacctcgtc gctgccgctc gacatcctga
     6301 ccaaggtgga tcgcatgagc atggcccact cggtggaggc gcgcgaaccg ctcctcgatc
     6361 accgtctcat cgagtgggcc gccacgctcc cgcccgagga ccggctgcgc ggcaccacca
     6421 ccaagtggat cttccgccag gcgatccggg acatggtgcc gcaggcgatc ctcgaccggc
     6481 cgaagcgcgg cttcgccgtg ccgctcggcc gctggttccg cggtcgcctc gacggcacgc
     6541 tgcgcgacct gctcctgtcg cgccgcagcc gcgagcgcgg ggtgttcgag ccgcgcgaga
     6601 tcgagcggct gatcgccgcg cacgagcgcg gccgccccct cgacctcgag ctgtggaccc
     6661 tgatgtcgtt cgagctctgg tgccgcgcct tccaggacgc ggggccgcgg accctgcggg
     6721 aggtctcctg tgcctgaggc gatcctgacg gcggcgcgcc cgctgcgcat cgtcatggtc
     6781 gcccccggtc gcggcgccac cggcggccag gagacgcagg cctccaccct cgaggatcgg
     6841 ctgcgcgccg agggacaccg cgtgcgccgc atcccgatcg atccggcgcc gccgcacgcg
     6901 ctgcggtggg cgcgggacgt ccgctacgcc cgcaccatcc tgaacgaggc gctctacgtc
     6961 cgatcgctcg ccgccctgcg ccgcgccgac gtggtgcacg tcttctccgc gtcgtactgg
     7021 tcgttcctgc tggcccctct gccggcgctc ctggccgggc ggcttttcgg gcgtcgggtc
     7081 atcctccatt accacagcgg cgaggcggag gagcacctgg ggcgctgggg gatgctggtg
     7141 cacccctggc tgcggctcgc cgacgcggtg gtcgtcccga gtctgtacct gcgcgaggtg
     7201 ttcgcggtgc acgggtacca cacccgccac atcccgaaca tcatcgagac cgatcgcttc
     7261 cgcttccgcc cccgcatgcc gctgcagccg cgcttcgtgt cgacgcgcaa cctcgagccc
     7321 ggctaccgcg tggacgtgac gatccgggcg ttcgcgttgc tgcgcgagcg gtacccggac
     7381 gcgacactgc tcgtcgccgg gcgcgggagc gaggaggatc ggctgcgggc gctcgcctcc
     7441 gaggtcgggc cgcaggggat ccgcttcctc ggggcgctcg atcgctcaca catgccgcag
     7501 ctcctggagc ggggcgacat cctgctcaac gcctcggtca tcgacaacca gccggtgtcg
     7561 atcctcgagg cgttcgccgc ggggctcctg gtggtcacca ccgtgcccgg ggacctgcgc
     7621 cacatggtgc gcgacggcga gaccggccgc atcgtcccgc ccgcgaaccc caaggcgatg
     7681 gctcaggcgg ccgcggccct cctcgaggat ccggagcggg ccgcgcggat cgccgcgcgc
     7741 gcccggcgcg aggcgggccg gcattcctgg ggcgccgtgc gggacgcctg gatgaaagcc
     7801 tacggatgtc cggtcgcggc ggcgggatcg gccgggatgg cgggggcggc ggcgtgatgc
     7861 gcatcgagcg gtggacctcg atcgggccgc gggagatcgc ccaccgcggg cgccaggagg
     7921 tgtcgaagtg gatcgatcgg atcgtcccgc cggcggcccg gatgccgctc caggaggccg
     7981 cgccgccggc ggccgccccg gccgcgccgc cgttcttcgc cggcctcgac agcccgctcg
     8041 tccgggagat gttcgagggc cgcctcgggg tggcgcgggc gcagctcctc gcggccgccc
     8101 gccgcttcgc ggagggac
//
LOCUS       DASRIQ010000246         5734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2974970, whole genome shotgun sequence.
ACCESSION   DASRIQ010000246 DASRIQ010000000
VERSION     DASRIQ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2974970"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFD06_06090"
     CDS             complement(<1..696)
                     /locus_tag="VFD06_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03141.1"
                     /translation="MTVSARLSLLVCLFAGILLLSAATPPEKPLEEEGEAAEEEGLAP
                     QVDIGALATAPPPAQAPSIQAPSLPEKPAAGMGRLRIDVEGTRRWCTFPDDRVVKPPH
                     KPSAPAGRSPVYTFGYMFTVAAVNRARPSETVMLFESPVIRTAVLRQAAKLGQGQAAP
                     PNKPPVIGDMPEKVKAEKKLDPSAMVPFWQEAYRCTKLPEAFDFDVPPGTYDFYLAFD
                     ILLRSGNWTHRTIA"
     gene            1038..3407
                     /locus_tag="VFD06_06095"
     CDS             1038..3407
                     /locus_tag="VFD06_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZN03142.1"
                     /translation="MDEKLCSDLEFGAILEGLQEETATPLGALRAAALRPYRDADDVA
                     REQRITLEALAHLEQRGTLPFGTLLDLTPVIDRLGLEGQELGAREVLDLLALMRSGRD
                     LRGALASAREAFPGLWRLTADLPDLGNLFRFLDGKIGPQGEVLDHASDTLGGLRADLR
                     RAGARLETVLAGVLQRPEVERALQDDFVALRSERHVIPIRTESRGAVAGIVHAVSGSG
                     ATVFVEPLETVELNNEIVTLREREAAEVRRLLREYSDLLRGRLSELRALAHGVGGLDL
                     AMARARLARRQGGVAAAIAPDDVVDLRGARHPLVEASLRSEGASIVPLDLSLGPDGKV
                     LVISGPNTGGKTVALKTLGLLVLMHQSGLLVPAREAALPVYGSVHLDIGDRQSIADRL
                     STFSARVRAIGRMSETLAPPALVLLDEVGSGTDPEEGVALAIAVIDHFRRRGARVLAT
                     THLEALKAYAATTPGCGNAAMQIDEASGTPTYRLALGVPGRSSALEMAARLGLPAGIL
                     EEARARRGAAGRMLDEYLRRLEVLTADLEQKTAAAERESARLVSERAAFERGLEELEA
                     RMRERVAKEIERAVADARAAGERYLAGLRDGEIAGRLRREEDRLAARLRDEAHQRILE
                     ITGRTAPAAPGPERLRPGIRVRVRGLGLIAEVESIQGDRVAVVARGKRLLVPRSDCAP
                     EPAAAAGRVSALPDGVRLERRPEASPSRAGGIDLRGVKVEEALERLDKFLDDASVDGL
                     DQVRLLHGVGSGRLRRAVHDLLGDHPHVAAFAPASADDGGDGTTIVTLR"
     gene            complement(3424..4284)
                     /locus_tag="VFD06_06100"
     CDS             complement(3424..4284)
                     /locus_tag="VFD06_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN03143.1"
                     /translation="MRAGRWISILAAGCVLVGAGIRAGQEPGVVLLDAPRGGWLGEIR
                     AGAPLEVIEERDGWRKVRIEGWVREMPTPGSPASAGAAPAGPAGSAPAPGVSAAGVAA
                     AAAPGASRISGLLSPRPGMQPATAGGGLVVLLLADPDATDAAHRALGEPCRADLAAMD
                     TRLEDLAKDVQAALNSSSNFKEAAQRNDRAKAALATAQRERAARLQQCREQAEGFFSE
                     RALARTVTDAAGRFELQPVEPGRYRVVAMERTPSGTRAWSMSAEVRATEGAVLEGRAA
                     NGPDPYWDLR"
     gene            complement(4304..5098)
                     /locus_tag="VFD06_06105"
     CDS             complement(4304..5098)
                     /locus_tag="VFD06_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03144.1"
                     /translation="MRCVAIAAAALLPPAAPDAAAAPPGGEDRLLVGFIEDGALINQA
                     WIEFAGTLRDAEDGRDLGLGVRAAFRYGRDAEAGFDLAVLNRERDEGALLYGSPVPDR
                     FEHTGAADPVVYGKYRLVRGACDLALGARASLPLAPHDSGLTSGAWEGRGFVAFRGAW
                     RQAAFVGHLGLGVSGDAQYEDGAEGGTFATGSIGAVVAVARLWNLLAEFNFEGARFRG
                     EEERGRGLVGIDWQPTRNLRVRGGIGSGWGGESGTISGIAAFVFNL"
     gene            complement(5393..>5734)
                     /locus_tag="VFD06_06110"
     CDS             complement(5393..>5734)
                     /locus_tag="VFD06_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03145.1"
                     /translation="LLEFKADSEAKTEEIRRNQERYQALRQRAMKEIGLLRKRLREAE
                     IALEERRIEMAVLEERLRRGETPAPERGGPALHELLAEHDDRPAAAAGAPAVLGGWTG
                     GFPPLDRKDDH"
BASE COUNT          766 a         2119 c         2074 g          775 t
ORIGIN      
        1 agcgatggtg cggtgggtcc agttccccga tcggagcagg atgtcgaacg cgaggtagaa
       61 gtcgtaggtc ccgggcggga cgtcgaaatc gaacgcctcc gggagcttcg tgcagcggta
      121 cgcctcctgc cagaatggca ccatggccga cgggtccaac ttcttttcgg ccttcacctt
      181 ctccggcata tcgccgatca ccggcggctt gttcgggggg gccgcctgtc cctgccccag
      241 tttcgccgcc tggcgcagca cggcggtccg gatgaccggc gactcgaaga gcatgaccgt
      301 ctccgacggg cgcgcccggt tcaccgccgc caccgtgaac atgtagccga acgtgtagac
      361 cgggctgcgc cccgcggggg cgctcggctt gtgcggaggc ttgacgacgc gatcgtccgg
      421 gaaggtgcac cagcggcgcg ttccttcgac gtcgatccgc aggcgtccca tgccggccgc
      481 gggcttttcg ggcaggctcg gcgcctggat cgacggcgcc tgcgcggggg gcggggcggt
      541 cgcgagcgct ccgatgtcca cctggggggc gaggccctcc tcctcggccg cctccccctc
      601 ctcctcgagt ggtttttcgg gaggcgtggc ggcgctgagc agaaggatcc cagcgaacag
      661 gcagacgagc agggacaggc gcgcggacac ggtcatgccg atctcaaagg gtggcgatta
      721 tactgccggt ttcagaaccg ggtgtccgtt ctcgggggcg agggacgaag cgatgccgtt
      781 cccggagctg ccccatcctc gctggtcgag gttgacggac gcgggaccgg ggactgccat
      841 ccccggtcga taaggcgcgg gagggtcagg gcggaatggg ttccgaggcg tggtcaactt
      901 caggaaggcg ggcagctccg ggaaattcag catcgctctc ctgggagggg tctgcaagga
      961 cccctcccgc ggagcgcact ttacccattc cgtagggccg agtcaacacc tcctcctcaa
     1021 ttagttgggg tccaaggatg gacgaaaaac tctgctccga cctggaattc ggcgccattc
     1081 tcgaggggct ccaggaggag accgcgacac cgctgggggc gctgcgggcg gcggctttgc
     1141 gcccctaccg ggatgccgac gacgtagccc gggagcagcg gatcaccctg gaagcgctcg
     1201 cgcacctcga gcagcgtggg acgctgccgt tcgggacgct cctggatctg acccccgtga
     1261 tcgaccgcct ggggctcgag gggcaggagc tgggggcgcg cgaagtcctc gatcttctgg
     1321 ccctgatgcg ctccggacgc gatctccggg gcgcactcgc ctcggcgcgc gaggcgttcc
     1381 cggggctctg gcgcctgaca gccgatctcc ccgacctcgg gaatctgttc cgcttcctcg
     1441 acggcaagat cggaccccag ggggaggtgc tcgatcacgc cagcgacacg ctcggcggcc
     1501 tgcgcgccga cctgcgccgc gccggcgcgc ggctcgagac ggtgctggcg ggtgtgctcc
     1561 agcgcccgga ggtcgaacgc gcgctccagg acgatttcgt ggcgctgcgc agcgaacggc
     1621 acgtcatccc gatccgcacc gagagccgcg gcgcggtcgc ggggatcgtg cacgcggtct
     1681 cgggaagcgg cgccaccgtc ttcgtcgagc cgctcgagac ggtcgagctc aacaacgaga
     1741 tcgtcacgct gcgcgagcgg gaggcggcgg aggtgcggcg gctcctgcgc gagtactccg
     1801 atctgctgcg cggaaggctc tcggagctgc gggcgctggc gcacggcgtc ggcggactcg
     1861 acctcgcgat ggcgcgggcc cgcctggcgc ggcgccaggg gggcgtcgcg gccgcgatcg
     1921 cgccggacga cgtcgtcgac ctgcgcggcg cccgccatcc gctcgtcgag gcgagcctgc
     1981 ggagcgaggg ggcgtcgatc gtgcctctcg atctgtcgct cggtccggac ggaaaggtgc
     2041 tggtgatcag cggcccgaac accggcggca agacggtggc gctcaagacg ctcgggctcc
     2101 tcgtcctgat gcaccagtcg gggctgctcg tcccggcgcg cgaggcggcg cttccggtct
     2161 acggctcggt ccacctcgac atcggcgatc gccagtcgat cgccgatcgt ctcagcacct
     2221 tctcggcgcg cgtgcgggcg atcggccgga tgtcggagac cctcgcgccg ccggcgctcg
     2281 tcctcctcga cgaggtcggc agcggcaccg accccgagga gggggtggcg ctggcgatcg
     2341 ccgtcatcga tcacttccgg cggcggggcg cgcgcgtgct cgccacgacg cacctcgagg
     2401 ccctcaaggc gtacgcggcg accacgcccg ggtgcggtaa cgccgccatg cagatcgacg
     2461 aggcgtcggg gactccgacc taccgcctgg cgctcggggt gcccggccgc tcgagcgccc
     2521 tggagatggc ggcgcgcctg ggactccccg ccgggatcct cgaggaggcg cgcgcccgcc
     2581 gcggcgccgc gggacggatg ctcgacgagt acctgcgccg cctcgaagtc ctgacggccg
     2641 acctcgagca gaaaaccgcc gccgcggagc gcgaatcggc gcggctggtc tcggaacggg
     2701 ccgcgttcga gcgcggcctc gaggagctcg aggcgcggat gcgcgagcgc gtggcgaaag
     2761 agatcgagcg cgccgtcgcc gacgcccgcg ccgccggcga gcgctatctc gccggcctgc
     2821 gcgacggcga gatcgccggg cgtctgaggc gcgaggagga tcgcctggcg gcgcgcctcc
     2881 gcgacgaggc ccaccagagg atcctcgaga tcaccggccg caccgccccc gccgcgcccg
     2941 ggcccgagcg gctgcggccg gggatccgcg tgcgcgtgcg cggcctcgga ctgatcgcgg
     3001 aggtcgaatc gatccaggga gatcgcgtcg cggtggtcgc acgcggcaag cggctgctcg
     3061 tgccgcgatc cgactgcgcg ccggagccgg ccgcggcggc ggggcgcgtc tccgcgctcc
     3121 ccgacggggt acggttggag cggcggccgg aggcatcccc gtcgcgcgcc ggcggcatcg
     3181 acctgcgcgg ggtgaaggtg gaggaggcgc tcgagcgact cgacaagttc ctcgacgacg
     3241 cgagcgtcga cgggctcgat caggtgcggc tgctgcacgg cgtcggatcg ggacgtctgc
     3301 gccgggcggt gcacgacctg ctcggagacc atcctcacgt cgccgccttc gcgccagcct
     3361 cggcggacga cggcggcgac gggacgacga tcgtcaccct gcgctgaccg gggcgcgatc
     3421 cttctagcgc aggtcccagt acggatcggg gccgttcgcg gcacgccctt cgagcaccgc
     3481 cccctccgtc gccctgacct cggcgctcat cgaccaggcg cgcgtccccg agggggtgcg
     3541 ctccatggcg accacgcggt agcggcccgg ctcgaccggc tgaagctcga agcgcccggc
     3601 tgcgtcggtg acggtgcgcg cgagggcgcg ctccgagaaa aacccctccg cctgctcccg
     3661 gcactgctgc aggcgggcgg cgcgctcgcg ctgcgccgtc gcgagcgccg ccttggcccg
     3721 gtcgttgcgc tgcgccgcct ccttgaagtt cgagctcgag ttgagagccg cctggacatc
     3781 cttggcgagg tcctcgagcc ttgtgtccat cgcggcgagg tcggcacggc aaggctcccc
     3841 gagcgcccgg tgcgccgcgt cggtcgcgtc gggatcggcg agcagcagca ccaccagccc
     3901 tccgccggcg gtggcgggct gcatcccggg ccgcggggag aggagcccgg agatgcgcga
     3961 cgcgccaggc gccgccgcgg ccgccacccc cgctgccgac acgcccggcg ccggcgcgct
     4021 tccggcggga cccgccggcg ccgctccagc ggacgccggg gatccggggg tcggcatctc
     4081 gcggacccag ccctcgatcc ggaccttccg ccagccgtct ctctcttcga tcacctccag
     4141 gggcgccccc gcccggatct cgccgagcca gccgccgcgc ggcgcgtcga gcaggacgac
     4201 ccccggctcc tgtcccgccc tgattcccgc cccgaccaag acgcaacccg ctgccaggat
     4261 ggaaatccaa cgtcctgcgc gcatgactcc tccttactcg tcgtcagaga ttgaacacga
     4321 aagcggcgat ccccgagatt gtcccagatt ctccacccca gcccgagccg atgcccccgc
     4381 gcacccggag gttcctcgtc ggctgccagt cgatccccac gagcccccgg ccgcgctctt
     4441 cctcgcctcg aaagcgggcc ccctcgaagt tgaattccgc caggaggttc cacagccggg
     4501 ccacggcgac caccgcgccg atcgatccgg tcgcgaacgt gccaccctcc gcgccgtcct
     4561 cgtactgtgc gtcgccgctc acgccgagcc cgagatgccc cacgaaggcc gcctgccgcc
     4621 aggcccccct gaaggcgacg aagccccggc cctcccacgc gccggaggtg agtccggagt
     4681 cgtgcggggc cagaggcaac gacgcgcggg cccccagcgc caggtcgcag gcaccgcgca
     4741 ccagccggta cttgccgtac accacgggat cggcggcgcc cgtgtgctcg aatcgatccg
     4801 ggaccggact cccgtacagg agcgcgcctt cgtcgcgctc ccggttcagc accgcgagat
     4861 cgaacccggc ctccgcatcg cggccgtagc ggaacgccgc gcgcacgccg aggccgagat
     4921 cccggccgtc ctccgcgtcg cgaagcgtcc cggcgaactc gatccaggcc tggttgatga
     4981 gggcgccgtc ctcgatgaaa ccaacgagca gccgatcctc ccctcccggc ggcgcggcgg
     5041 ccgcgtccgg cgccgcgggc gggaggagcg ccgccgcggc gatcgcaacg cagcgcacga
     5101 ggcgaaaggc acgcggtccg cttgcgcggc gggcggcggt ccctatattc gccgggggac
     5161 ccggcgcggt atgcggtccg ccgggacggg ggcggtgacg aaagcgcatc tcatccggac
     5221 gatccgcagg gaatgcgatc ggcgctcgct gcacagcggg cccgattgct cctgccgacc
     5281 gcgccgctcg cccgcctccg gcggcgggct cacgaagcgg agccggccgc gctgccggcc
     5341 gcgcggcgca gggggcggtc cctcgcgctg agccaggagc ctgtccaggc tcctagtgat
     5401 cgtccttgcg atcgagcggc gggaatccac cggtccaccc gcccaggacg gccggcgcgc
     5461 ctgcagcggc ggcgggacga tcgtcgtgct ccgccagcag ctcgtgcagc gccggaccac
     5521 cgcgctccgg ggcgggagtc tcgccccggc gcagccgctc ctcgagcacc gccatctcga
     5581 tgcggcgctc ttccagggcg atctccgcct cgcgcagccg cttgcgcaga agcccgatct
     5641 ccttcatggc gcgctgccgc agtgcctggt agcgctcctg gttccgccgg atctcctccg
     5701 tcttcgcctc ggagtcggcc ttgaactcca ggag
//
LOCUS       DASRIQ010000247         6835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3768657, whole genome shotgun sequence.
ACCESSION   DASRIQ010000247 DASRIQ010000000
VERSION     DASRIQ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3768657"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFD06_06115"
     CDS             complement(<1..299)
                     /locus_tag="VFD06_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZN03146.1"
                     /translation="MNRPPRWMEADRRIVDAALRRLLPRPGAVPPTVRRAMAYSLFPG
                     GKRLRPVLALEACRVVGGRLRDVLPAAAAIEMIHTYSLVHDDLPALDNDDLRRGRP"
     gene            complement(296..649)
                     /gene="xseB"
                     /locus_tag="VFD06_06120"
     CDS             complement(296..649)
                     /gene="xseB"
                     /locus_tag="VFD06_06120"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZN03147.1"
                     /translation="MSLPPKDSEEPRARRARAKEPSFEAALERLEEIVVLLEAGDRPL
                     QESLKLFEEGIALTRQCADRLDHAQRRIELLTRDAQGRPVLLPFAPGEEPAGDPGADE
                     ESGGAAETDDDGVES"
     gene            complement(624..1883)
                     /gene="xseA"
                     /locus_tag="VFD06_06125"
     CDS             complement(624..1883)
                     /gene="xseA"
                     /locus_tag="VFD06_06125"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZN03148.1"
                     /translation="MTGLFDTAAGEAARPTRRPLTVSELNAWVGALLEEGVPQVKVEG
                     EISNLRRYPSGHTYFTLKDADAQVAAVLFRGNALGLRFRPADGAHVVVRGRVGLYQAR
                     GAYQIVVEGMEPAGLGALQRALEALRERLRAEGLFEAARKRPLPRLPRCIGIVTSPRG
                     AALKDILRVLERRFDGLRIIVAPARVQGEGAGREIAGALGDLDRVREVDVVIVTRGGG
                     SIEDLWAFNEEIVARAIAASRVPVISAVGHEIDVTLADLVADLRAPTPSAAAEMVVLS
                     RLELFDRLASLRARLASAARYLVAEARRRFERVRTDRALREASARLREAIFRADDLGA
                     RLRAAAAGRTRDGRHRLERLAGRLGALSPLAVLARGYAVCTDVTGTILREAGGVGAGA
                     PVRVRLHRGRLFCEVKEVEDVAPPEGL"
     gene            complement(1867..2676)
                     /locus_tag="VFD06_06130"
     CDS             complement(1867..2676)
                     /locus_tag="VFD06_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HZN03149.1"
                     /translation="MRILMLGDVYGRPGRRILQEHLPRLRADRRIDFVVANVENAADG
                     FGVTPDLAQQLRAAGVDCLTSGNHIWDKPEILDYLRAEPRLLRPFNYPDGAPGAGLFA
                     GEAADGTPVAVVNLMGRLFMPPCDDPFRAAERALQEIGGRTKVILVDMHAEATSEKQA
                     IAVFLDGRVSAVCGTHTHVQTADERLLPGGTAYLSDLGMTGPYDSVIGIEKDLAIRRF
                     LSGMPVRFTVARHDPRLCGALVEIDPATGRALSIERVQLRPPGPGVDDRPV"
     gene            complement(2685..4238)
                     /gene="rny"
                     /locus_tag="VFD06_06135"
     CDS             complement(2685..4238)
                     /gene="rny"
                     /locus_tag="VFD06_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HZN03150.1"
                     /translation="MIPILIASALALAAGAAAWLLLRAGRGLVARAADRARVLEDEAE
                     MRVRLRQKEADLEIEERRAAVEAQAERQAQARREELRAQEDRVREQERNLQRRLQLLG
                     QKEREVEGREATVKDREAVAGEREREAQSALLRQRQALERIAGLTAKEARVELVQQIE
                     SEARHEAALSVRRIEEEARDGAMAAARRLTAEAVQRLPTGEIVDNVVTVVMLPNEEMK
                     GRIIGREGRNIRALEMATGVDLIVDETPQAIVLSCYDPFRRAVAQAALDRLIEDGRIH
                     PARIEEVVQKTRIDLEEGLEAAGESAAFDLGLTGLPPRLTRLLGRLRYRVVLGYNLLS
                     HSTTVARLGQQMATLLGARSDVMLRAGLLHEIGHVEEVQDGAPHPWVLSADLAAKFGE
                     DARVVQAIRALQTPDAAPSVEAVLLQVAERAVVARPGERDDNLDVFIERLRRLEEIGA
                     SFSGVSRVFAMRAGKEVRVIVEPASVGDTEVAWLSKDITRRIAQEVQFPGSVRVSVIR
                     ETRAVDYAT"
     gene            complement(4327..4626)
                     /locus_tag="VFD06_06140"
     CDS             complement(4327..4626)
                     /locus_tag="VFD06_06140"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZN03151.1"
                     /translation="MDRPTSHLIQVEIYGQSYNLRGEGDTSYLQELAGYVDRRMREVS
                     EATATVDSLKVAILAALNIADEGRRERPDAASRLPAANERIARMIDRLDRGLAEA"
     gene            complement(4650..4973)
                     /locus_tag="VFD06_06145"
     CDS             complement(4650..4973)
                     /locus_tag="VFD06_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03152.1"
                     /translation="MEAKKVVVDLQQLDLLEERIQRAVEILAGMRRERDTARAEAKEL
                     RERCDRLQAEQRRLEQERQGARGLTEQLDLLKEERQAIRGRVGRLLEMMAELEETGAP
                     ARADH"
     gene            complement(4994..>6835)
                     /gene="pheT"
                     /locus_tag="VFD06_06150"
     CDS             complement(4994..>6835)
                     /gene="pheT"
                     /locus_tag="VFD06_06150"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZN03153.1"
                     /translation="YGVPLRRPASGLKPGGPPTSGHATVEIAAPGRCGRYAARCVLGV
                     KVGPSPDWLRRRLERLGHRSINNVVDATNFVLWEQGQPLHPFDLDRVEKQSVIVRQAR
                     PGESLVTLDGVARRLSPDILVIADPRQALALAGIMGGQSSEIRDGTQNVLLESAWFDP
                     VTVRRGAKSLGMHTDASHRFERGADPEGVLPALDRAAALIADIAGGRVTDPPIDVHPS
                     PEPPRAVALRPSRTAALLGCDPGRGRMRAILERLEFVIAEETEDRLLARVPSFRRDVE
                     REVDLIEEVARHVGYDAIPSLLPRLDPVEHGRPEGLRRLDEARRALLQAGSSEAVNFD
                     LAEAEECRAFEPAIRPVAIENPLMSQAASLRTTLLPGLLRNVAHNLNRGLPAVQLFEV
                     GTVFHPGPDRPVERTRVAAVVAGRSPAPHWSAGRRDADLFDAKGAVESLAAALGVSRL
                     RFASDRIAPGAALVGPAIESEGALLGRVGEVAPEALRRYEIDVPVFAFEMDLDAWLAV
                     SAAGRIFRPLPRVPAVRRDLALVVPDRLTIEALEEAMVGASPLPVAGVTVFDRYRGKG
                     VPPGHASVALQLVFQDPGRTLEAEAVQKAIEAIVAEAERHGARLRGA"
BASE COUNT          876 a         2642 c         2422 g          895 t
ORIGIN      
        1 ggccggccgc ggcgcaggtc gtcgttgtcg agcgccggca ggtcgtcgtg gacgagggag
       61 taggtgtgga tcatctcgat cgccgcggcg gcggggagca cgtcgcgcag gcgtcccccc
      121 acgacccggc aggcctcgag cgccagcacc ggccgcagcc gctttccgcc ggggaagagc
      181 gagtacgcca tggcgcgccg caccgtcggc ggcacggccc ccggacgcgg gaggaggcgc
      241 cgcagcgcgg cgtcgacgat gcggcggtcc gcctccatcc agcgcggcgg acggttcacg
      301 attcgacccc gtcgtcgtcc gtctccgcgg cgccgccgga ctcctcgtcc gcgccgggat
      361 cgccggccgg ctcctccccg ggggcgaacg gcaggagcac cggccgcccc tgcgcgtcgc
      421 gcgtcaggag ttcgatgcgg cgctgtgcgt gatcgagccg gtcggcgcac tgccgcgtca
      481 gggcgatccc ctcctcgaac agcttgagcg actcctggag cgggcgatcc cccgcctcga
      541 ggaggacgac gatctcctcg agccgctcga gcgccgcctc gaacgacggc tccttcgcgc
      601 gcgctcgcct tgcgcgcggc tcctcagagt ccttcggggg gagcgacatc ctcgacctcc
      661 ttgacctcgc agaacaaacg gccgcggtgc aggcggaccc gcaccggcgc gccggcgccg
      721 acgccgcccg cttcgcgcag gatcgtcccc gtcacgtcgg tgcagacggc gtagcctcgg
      781 gccagcaccg cgagcgggga gagggccccg agacgtccgg ccaggcgctc gagacggtgg
      841 cggccgtccc gcgtccgccc cgccgcggcg gcccgcaggc gcgctcccag atcgtcggcg
      901 cggaagatcg cctcgcgcag ccgggccgac gcctcgcgca gcgcccgatc ggtgcggacc
      961 cgctcgaagc gccgccgcgc ctccgccacc aggtaccgcg ccgccgaagc gagccgggcg
     1021 cgcagcgacg ccagccggtc gaagagctcg aggcgcgaca gcaccaccat ttcggcggcg
     1081 gccgaagggg tcggggcgcg caggtcggcc acgaggtccg ccagcgtgac gtcgatctcg
     1141 tgcccgaccg ccgagatcac cgggacgcgg cttgcggcga tggcgcgcgc cacgatctcc
     1201 tcgttgaagg cccacaggtc ctcgatcgat ccgccgccgc gggtcacgat cacgacgtcg
     1261 acctcccgca cgcggtcgag gtcgccgagc gcgccggcga tctcgcggcc cgccccctcc
     1321 ccctgcaccc gcgccggcgc cacgatgatc cgcagcccgt cgaagcgacg ctcgaggacc
     1381 cgcaggatgt ccttgagcgc cgcgccgcgc ggagacgtga cgatgccgat gcagcgcggc
     1441 aggcgcggca gcggccgctt gcgcgccgcc tcgaagagcc cttcggcgcg cagccgctca
     1501 cggagcgctt cgagggcgcg ctgcagcgcc ccgagccccg ccggctccat cccctcgacg
     1561 acgatctggt aggcgccgcg cgcctggtac aggccgacgc gcccgcgcac gacgacgtgc
     1621 gccccgtccg cggggcggaa gcgcagtccc agggcgttgc cgcggaacag caccgcggcc
     1681 acctgggcgt cggcgtcctt gagggtgaag taggtgtggc cgctcgggta gcggcgcagg
     1741 ttgctgatct ccccctcgac cttgacctgg gggaccccct cctcgaggag ggcgccgacc
     1801 cacgcgttca gctcgctgac ggtgagcggg cggcgggtcg gccgcgccgc ctctcccgcc
     1861 gcggtgtcaa acaggccggt catcgacgcc tggtcccggc gggcggagct gcacgcgctc
     1921 gatcgacagg gctcgccccg tcgccggatc gatttcaacg agggctccgc acagccgcgg
     1981 gtcgtggcgc gccacggtga agcggaccgg catgccggag agaaagcgcc ggatcgccag
     2041 atctttctcg atgccgatga ccgagtcgta gggaccggtc atcccgaggt ccgacaggta
     2101 cgcggtgccg cccgggagca gccgctcgtc ggcggtctgc acgtgcgtgt gggtgccgca
     2161 caccgccgag acgcggccgt cgaggaagac cgcgatcgcc tgcttctcgg acgtcgcctc
     2221 ggcgtgcatg tccaccagga tcaccttcgt ccgcccgccg atctcctgaa gcgcccgctc
     2281 ggcggcgcgg aagggatcgt cgcacggagg catgaacagc cgccccatca ggttgacgac
     2341 cgccacaggg gtgccgtccg cggcctcgcc ggcgaagagt cccgcgccgg gggcgccgtc
     2401 cgggtagttg aacggacgga gcaggcgggg ttccgcgcgc aggtagtcga ggatctccgg
     2461 cttgtcccag atgtggttgc cggaggtcag gcagtcgacg ccggcggcgc gcagctgctg
     2521 cgccaggtcc ggcgtgacgc cgaacccgtc ggccgcattc tcgacgttgg cgacgacgaa
     2581 atcgatgcgg cgatcggccc ggaggcgcgg caggtgctcc tgcaggatgc ggcggccggg
     2641 ccggccgtag acgtccccca gcatcaggat gcgcacgcga cccctcaggt ggcgtagtcc
     2701 acggcgcggg tctcgcggat gacgctcacc cgcacgctgc ccgggaactg cacctcctgc
     2761 gcgatccgcc gggtgatgtc cttcgacagc caggcgacct cggtgtcgcc gacggaggcg
     2821 ggctcgacga tgacgcgcac ctccttgccg gcgcgcatgg cgaagacccg ggacacgccg
     2881 ctgaacgagg ccccgatctc ctccaggcgc cgcagccgct cgatgaagac gtcgaggttg
     2941 tcgtcccgct ccccgggacg tgcgaccacc gcgcgctcgg cgacctggag cagcaccgcc
     3001 tcgaccgagg gggcggcgtc gggcgtctgc agcgcccgga tcgcctgcac cacccgcgcg
     3061 tcctcgccaa atttcgcggc gaggtcggcg ctgagcaccc acggatgcgg tgcgccgtcc
     3121 tgcacctcct cgacgtggcc gatctcgtga agcagcccgg cgcggagcat gacgtcgctg
     3181 cgcgctccga ggagcgtcgc catctgctgt ccgagccgcg ccaccgtcgt cgaatgcgac
     3241 aaaaggttat agcccagcac cacgcggtag cgcagccgtc cgaggagccg cgtcagccgc
     3301 ggcggcagcc cggtgagccc gagatcgaag gcggccgact cgccggcggc ctccagcccc
     3361 tcctcgaggt cgatcctggt cttttggacc acctcctcga tgcgggccgg gtggatgcgg
     3421 ccgtcctcga tgaggcggtc gagcgccgcc tgtgccaccg cgcggcggaa cgggtcgtag
     3481 cacgacagga cgatcgcctg cggcgtctcg tcgacgatga ggtcgacccc ggtcgccatc
     3541 tccagggcgc ggatgttgcg cccctcgcgc ccgatgatgc gccccttcat ctcttcgttc
     3601 ggcagcatca cgacggtgac gacgttgtcg acgatctccc cggtggggag gcgttgcacc
     3661 gcctcggcgg tgagccgccg cgccgcggcc atcgccccgt cgcgcgcctc ctcctcgatg
     3721 cggcgcaccg acagcgccgc ctcgtggcgc gcctccgact cgatctgctg cacgagctcg
     3781 acgcgcgcct ccttcgccgt cagcccggcg atccgctcga gcgcctggcg ctggcgcagg
     3841 agggcgctct gcgcctcgcg ctcccgctcg ccggccaccg cctcgcggtc cttcacggtc
     3901 gcctcgcgcc cttccacctc gcgctccttc tgtcccagga gctgcagccg gcgctggaga
     3961 ttgcgctcct gctcgcgcac ccggtcctcc tgggcgcgca gctcctcgcg gcgcgcctgc
     4021 gcctgccgct ccgcctgcgc ctcgacggcg gcgcgccgct cctcgatctc gaggtccgcc
     4081 tccttctggc gcaggcggac gcgcatttcc gcctcgtcct cgaggacccg ggcccggtcg
     4141 gcggcgcgcg ccacgagccc cctgccggcg cgcagcagga gccacgcggc ggcgccggcg
     4201 gcgagcgcca gggccgaagc gatcagaatc ggaatcatcg ttctccccgc gtcgggcgtt
     4261 cgtgcgcggc cggccggatg gtgcggtgtt cgtggagggg atccggggtc ttttgggagg
     4321 gccccgtcac gcctcggcga ggccgcgatc gagccggtcg atcatgcggg cgatccgctc
     4381 gttcgccgcc ggcaggcggg aggccgcgtc cgggcgctcg cgccgtccct cgtcggcgat
     4441 gttcagcgcc gccaggatcg cgaccttcag cgagtcgacg gtggcggtcg cctcggacac
     4501 ctcccgcatg cggcgatcga cgtagccggc cagctcctgc aggtaggagg tgtccccctc
     4561 gccgcgcagg ttgtacgact gcccgtaaat ctcgacctgg atgaggtggc tcgtggggcg
     4621 atccatgcgg cctccgctcg gtgccggcgt cagtgatcgg cgcgcgccgg cgctccggtc
     4681 tcctccaact ccgccatcat ctccagcagc cggccgacgc ggccgcggat cgcctggcgc
     4741 tcctccttga ggagatccag ctgctcggtc aggccgcgcg ccccctgtcg ctcctgctcc
     4801 agccggcgct gctccgcctg cagccgatcg cagcgttcgc gcagctcctt cgcctcggcg
     4861 cgggccgtgt cgcgctcccg gcgcatcccg gccaggatct ccacggcccg ctggatccgc
     4921 tcctcgagca gatcgagctg ctgcagatcg accaccacct tcttcgcctc catccgtccc
     4981 tcctcgcgag ccttcacgcg ccccgcaggc gcgccccgtg tcgctccgcc tccgccacga
     5041 tcgcctcgat cgccttctgc accgcctcgg cctcgagcgt ccgcccgggg tcctggaaca
     5101 ccagctgcag cgccacgctg gcgtggccgg ggggaacccc ctttccccgg taccgatcga
     5161 acaccgtcac cccggcgacc ggcaggggac tggcgcccac catcgcctcc tccagcgcct
     5221 cgatcgtcag ccggtccggc accacgagcg cgaggtcgcg ccgcaccgcc ggtacccgcg
     5281 gcagcggccg gaagatcctc ccggccgcgg aaacggcgag ccacgcgtcg aggtccatct
     5341 cgaacgcgaa gaccggaacg tcgatttcgt accgccgcag agcctccggc gccacctcgc
     5401 cgacccggcc gagcaacgct ccctccgact cgatcgccgg tccgacgagc gcagcaccag
     5461 gcgcgattct atccgaagcg aagcggagcc gggagacgcc gagggccgcg gcgagcgact
     5521 cgaccgcccc cttggcgtcg aacaggtccg cgtcccgccg cccggcgctc cagtgcgggg
     5581 cgggggagcg gccggcgacg accgcggcga cccgggtgcg ctcgaccggt cgatccgggc
     5641 cggggtggaa gaccgtgccc acctcgaaca gctgcacggc gggcaagccg cggttgaggt
     5701 tgtgggcgac gttgcgcagg aggcccggga ggagggtcgt gcgcagcgac gccgcctgcg
     5761 acatcaacgg gttctcgatg gcgacggggc ggatcgccgg ctcgaacgcc cgacactcct
     5821 ccgcctcggc gagatcgaag ttcaccgcct cggaggaacc cgcctgcagg agcgcccgcc
     5881 gcgcctcgtc gagacgccgc agcccttccg ggcggccgtg ctccaccggg tcgaggcgcg
     5941 gcaggagcga cgggatggcg tcgtagccga cgtgccgcgc cacctcctcg atgagatcca
     6001 cctcccgctc cacgtcccgc cggaacgagg ggacgcgcgc cagcaggcga tcctccgtct
     6061 cctccgcgat cacgaactcc agccgctcga ggatggcgcg catgcgcccg cgccccgggt
     6121 cgcagccgag gagcgccgcc gtccggctcg gccgcagcgc caccgcgcgg ggcggctcgg
     6181 gcgagggatg gacgtcgatc ggcgggtcgg tgacccgccc tccggcgatg tcggcgatga
     6241 gcgccgcggc ccgatcgagc gccggcagga ccccttccgg atcggccccc cgctcgaagc
     6301 ggtgcgaggc gtcggtgtgc atcccgaggc tcttggcgcc gcgccgcacg gtgaccggat
     6361 cgaaccaggc gctctcgagc aggacgttct gcgtgccgtc ccggatctcc gacgactgcc
     6421 cccccatgat gccggcgagc gcgagcgcct ggcgcgggtc cgcgatgacc aggatgtccg
     6481 gcgacaggcg gcgcgccacg ccgtcgagcg tgacgaggct ctcccccggg cgggcctggc
     6541 gcacgatgac gctttgcttt tcgacccggt cgagatcgaa cgggtggagc ggctgcccct
     6601 gctcccagag gacgaagttc gtcgcgtcga ccacgttgtt gatcgaccgg tgcccgaggc
     6661 gctccaggcg ccgccgcagc cagtcgggag acgggccgac cttgaccccc agcacgcagc
     6721 gggcggcgta gcgcccgcac cgacccgggg cggcgatctc gaccgtcgcg tgcccggagg
     6781 tcggcggccc tcccggcttc agcccggagg ccggccgacg cagcggcacg ccgta
//
LOCUS       DASRIQ010000248         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3222520, whole genome shotgun sequence.
ACCESSION   DASRIQ010000248 DASRIQ010000000
VERSION     DASRIQ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3222520"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VFD06_06155"
     CDS             <1..602
                     /locus_tag="VFD06_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZN03154.1"
                     /translation="EMALLAEERAALATEKAALEARLAEMKSREDAPGSQRKEREQEA
                     KKLQATYEGLVADLKKEVKSGQIQVRQLRDGLRVNVSQDILFDSASAELDNNGTEVLG
                     RVAGQLKKSPHQILVIGHTDNKPIGVALAKQYPSNWELAGARASSVVRLFNRSGQSAK
                     RMLAVSVADAKPVASNDSPEGRAKNRRIEIRLRPIPAED"
     gene            complement(695..1267)
                     /locus_tag="VFD06_06160"
     CDS             complement(695..1267)
                     /locus_tag="VFD06_06160"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HZN03155.1"
                     /translation="MKHTRVLLAAMTVAVLIGGAVIAQDPDSQSRRIAAVLRGIEEPP
                     SISSTGTGMFRATIAPDASSISYTLSYADLEGDVLQAHIHLGQRGVNGGIAVFLCSNL
                     GNGPAGTQLCPGPRAGTISGTFTGADIVGPAAQGLTAAEFTELVRAIDRSVTYVNVHT
                     SKHTGGEIRGQVAGQINIAPTAPAPTAPPD"
     gene            1408..1884
                     /locus_tag="VFD06_06165"
     CDS             1408..1884
                     /locus_tag="VFD06_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HZN03156.1"
                     /translation="MTQRRIVSGGQTGVDRAALDAALDAGFPCGGWCPPGREAEDGPI
                     PGRYPLDEMPSGGYLERTIRNVEESDGTLVIHFGPLRGGTERTVFHCTRLGKPVLLVD
                     GDRVPPGPTADRAAVFIAQHRIAVLNVAGPRASQSPQGHHHAYATVTRLLGLIANP"
     gene            complement(1967..>2604)
                     /locus_tag="VFD06_06170"
     CDS             complement(1967..>2604)
                     /locus_tag="VFD06_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03157.1"
                     /translation="ISQHADLDAFSGATGGLTGSLTEGHTYTTPGEQLVNLQVLDDDG
                     GLLSPTTTVNVLDARGAILLLIDQIDVRIAGSTGSHRAAWQAVRDLLDGNLLGDPSNG
                     AIDKLDADDLEAAVLRVIQSIGALRNATLVTGTDTTDMQAMLALSAWSIAVEAQKDAI
                     AATNPPTAGEAKQLGRIQSSITTGAARLASNDWSGAAQKFHDAVKLAVALL"
BASE COUNT          402 a          869 c          935 g          398 t
ORIGIN      
        1 aggaaatggc cctgctggcc gaggagcggg ccgcgctcgc gaccgagaag gcggccctcg
       61 aggccaggct ggcggaaatg aagtcgcgcg aggacgcgcc cggctcgcag cgcaaggagc
      121 gcgagcagga agcgaagaag ctgcaggcga cctacgaagg cctggtcgcc gatctcaaaa
      181 aggaggtgaa gtccggacag atccaggtgc ggcagctgcg ggacgggctg cgcgtcaatg
      241 tctcgcagga catcctgttc gattcggcgt cggccgagct cgacaacaac ggcaccgagg
      301 tcctgggacg cgtggccggg cagctcaaga agtcgccgca ccagatcctg gtgatcggtc
      361 acacggacaa caagccgatc ggagtggcgc tggcgaagca gtacccgtcg aactgggagc
      421 tggccggcgc ccgtgcctcg agcgtcgtgc gcctcttcaa ccgatcgggc cagtcggcga
      481 agcgcatgct cgccgtctcg gtggccgacg cgaagcctgt cgcgtccaac gacagcccgg
      541 aaggacgcgc caagaaccgc cgcatcgaga tccggctgcg gccgatcccg gcggaggact
      601 gagggcgcct cgtcctggac cgccatccac cgggatggcg ccctcgccgc tcagggcgcc
      661 ggcgtcggcc ggcgccctga gcgttcctgt tgcttcagtc cggcggggca gtcggggccg
      721 gggcggtcgg agcgatgttg atctggccgg cgacctggcc gcggatctcc ccgcccgtgt
      781 gcttgctggt gtgcacgttg acgtaggtga cgctccggtc aatggcgcgc accagctccg
      841 tgaactcggc cgcggtcagc ccctgcgcgg ccgggccgac gatgtcggcg ccggtgaagg
      901 tgccgctgat cgttcccgcc cggggtcccg ggcagagctg ggtcccggcc gggccgttcc
      961 ccaggttgct gcacaggaag acggcgatgc cgccgttgac gccgcgctgc ccgaggtgga
     1021 tgtgcgcctg cagcacgtcc ccctcgaggt cggcatacga cagcgtgtag gagatggacg
     1081 aggcgtccgg ggcgatcgtc gcgcggaaca tcccggtccc cgtcgaggag atcgaagggg
     1141 gctcttcgat cccgcgaaga acggcggcga tcctccgact ctgggaatcc ggatcctggg
     1201 cgatcacggc tcccccgatc aggacggcga cggtcattgc ggcgagcagg actctggtgt
     1261 gtttcatcgg gcttcctcca ctccgccgaa gcggtcgatg aattgatcgt gaaccagcag
     1321 attcaccctc gcaccggggc ccggtctgtg atccaggaca cactcggagg ctcagggaaa
     1381 atactcaacc gcgatgcaga atgagacatg acgcagcggc ggatcgtctc gggaggacag
     1441 accggcgtcg atcgcgccgc cctcgacgcc gccctcgacg cggggtttcc gtgcggtggc
     1501 tggtgccctc cgggccgcga ggccgaggac ggcccgattc ccgggcgtta tccgctcgac
     1561 gagatgccgt ccggcggata tctcgagcgg acgatccgca atgtcgaaga gagcgacggc
     1621 acgctcgtga tccatttcgg accgctgcgt ggcgggacgg agcgaaccgt cttccattgc
     1681 acgcggcttg gaaagcccgt cctcctcgtc gacggcgatc gcgtcccccc cgggccgacc
     1741 gccgatcggg ccgcggtctt catcgcgcag caccggatcg cggttctcaa cgtcgcgggg
     1801 ccgcgggcga gtcagagtcc gcagggtcac caccacgcct acgccaccgt cacacgactc
     1861 ctcggcctga tcgccaatcc gtgaacctga acgaggcccc ggaaaaacgc cggggggcgc
     1921 ccctcggatc gggacgcccc ccggttggtg gttgctactt tcccggctag agaagcgcga
     1981 cggccagctt cacggcgtcg tggaacttct gcgccgcgcc ggaccagtcg ttcgaggcga
     2041 gcctggcggc tcccgtggtg atcgaggact ggatgcggcc gagctgcttc gcttcgcccg
     2101 ccgtgggggg gttggtcgcc gcgatcgcgt ccttctgagc ctcgactgcg atcgaccagg
     2161 ccgacagcgc gagcatggcc tgcatgtcgg tcgtgtcggt cccggtgacg agcgtcgcgt
     2221 tgcgcagggc cccgatcgac tggatgaccc gaaggacggc ggcctccagg tcgtcggcgt
     2281 cgagcttgtc gatcgctccg ttggagggat cgcccagcag gttgccgtcc agcagatccc
     2341 gcaccgcctg ccaggcggcg cggtgactcc ccgtcgagcc ggcgatccgc acgtcgatct
     2401 gatcgatcag cagcaggatg gcgccgcgcg cgtccagcac gttgacggtc gtggtcgggc
     2461 tcagcaggcc gccgtcgtcg tccaggacct ggagattgac gagctgctcg ccgggggtcg
     2521 tgtaggtgtg accctcggtc aggctgccgg tcaggcctcc cgtggcgccg ctgaaggcgt
     2581 cgagatctgc gtgctgggag atgg
//
LOCUS       DASRIQ010000249         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2179501, whole genome shotgun sequence.
ACCESSION   DASRIQ010000249 DASRIQ010000000
VERSION     DASRIQ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179501"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..>2176
                     /gene="secA"
                     /locus_tag="VFD06_06175"
     CDS             26..>2176
                     /gene="secA"
                     /locus_tag="VFD06_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZN03158.1"
                     /translation="MVDTILKALFGTKNERELKKIVPRVAAINALEAEVAPLSDAAIR
                     ERVEGLRVRVRDGAALDDVLNEMFALTREAAKRTVRMRHFDVQLIGGMVLHHGKIAEM
                     KTGEGKTLVATLAASLNALAEKGVHVITVNDYLARRDASWMGPIYAFLGLSVGVIQHD
                     MDDASRQAAYAADITYGTNNEFGFDYLRDNMKYSLGAMVQRGHPFAIVDEVDSILIDE
                     ARTPLIISGPSDESTDLYARAAAVIPRLERESHFTVDEKARTVALTEDGVERCEQMLK
                     VENLYDPAQMELNHAAQQALRAHVLYRRDVDYVIKDGQIIIVDEFTGRLMPGRRWSDG
                     LHQAVEAKEKVAIQRENQTLATITFQNYFRMYGKLAGMTGTADTEAVEFDRIYKLEVM
                     VIPTNRTLIRIENPDVVYRTEREKFDAVVEEIKELYERRQPVLVGTISIEKSEHLSTM
                     LHKKKIPHVVLNAKYHEREAEIVAQAGRLGAVTIATNMAGRGTDILLGGNAEFVARRQ
                     LLEGREPGNVPPPEELAAAIEEARQACLTEHDEVVGLGGLHILGTERHEARRIDSQLR
                     GRAGRQGDPGSSRFYLSLEDDLMRIFGSDRISGLMQRLGMEEGVPIEHRMVTNAIERA
                     QKQVEARNFEVRKHLLEYDDVNNKQRTEIYRLRRELLEGKGQKEYILTKAEEILDHII
                     DSTCNPQADPDEWEPENLRNGMLRYFGVDTNQAGI"
BASE COUNT          424 a          737 c          724 g          291 t
ORIGIN      
        1 cgaagtcgtt tatcataagc cgatgatcgt cgacaccatc ctcaaggcgc tgttcgggac
       61 caagaacgag cgcgagctca agaagatcgt cccgcgcgtc gctgccatca acgccctcga
      121 ggcggaggtg gcccccctct ccgacgcggc catccgcgag cgggtcgaag ggctgcgcgt
      181 ccgggtgcgc gacggcgcgg cgctggacga cgtcctcaac gagatgttcg ccctgacccg
      241 cgaggcggcg aagcggaccg tccgcatgcg tcacttcgac gtccagctca tcggcggcat
      301 ggtcctgcac cacgggaaaa tcgccgagat gaagacgggg gaggggaaga ccctcgtcgc
      361 caccctcgcg gcgtcgctca acgcgctcgc cgaaaagggc gtgcacgtca tcacggtgaa
      421 cgactacctc gcccgccgcg acgcctcctg gatggggccg atctacgcgt tcctgggcct
      481 ttcggtcggg gtcatccagc acgacatgga cgacgcatcg cgccaggccg cctacgccgc
      541 cgacatcacc tacggcacca acaacgagtt cggcttcgac tacctgcgcg acaacatgaa
      601 gtactcgctc ggcgccatgg tgcagcgcgg ccaccccttc gccatcgtcg acgaggtgga
      661 ctcgatcctc atcgacgagg cgcgcacgcc cctcatcatc tccgggccgt ccgacgagtc
      721 gaccgatctc tacgcccggg ccgccgcggt catcccgcgt ctcgagcggg agtcccactt
      781 cacggtggac gagaaggcgc gcaccgtggc gctcaccgag gacggggtgg agcgctgcga
      841 gcagatgctc aaggtcgaga acctctacga cccggcgcag atggagctga accacgcggc
      901 gcagcaggcg ctgcgggcgc acgtgctcta caggcgggac gtcgattacg tcatcaagga
      961 cggccagatc atcatcgtcg acgagttcac cgggcgcctg atgccggggc gccgctggag
     1021 cgacgggctg caccaggcag tcgaggcgaa ggagaaggtc gccatccagc gcgagaacca
     1081 gacgctcgcg accatcacct tccagaacta cttcaggatg tatggcaagc tcgccggcat
     1141 gaccgggacc gccgacaccg aggcggtcga gttcgaccgc atctacaagc tcgaggtcat
     1201 ggtcatcccg accaaccgga cgctcatccg catcgagaac ccggacgtcg tgtaccggac
     1261 cgagcgggag aagttcgacg cggtggtgga ggagatcaag gagctgtacg agcggcgcca
     1321 gccggtgctg gtcgggacca tctccatcga gaagtcggag cacctcagca cgatgctcca
     1381 caagaagaag atcccgcacg tggtgctgaa cgccaagtac cacgagcgcg aggcggagat
     1441 cgtcgcgcaa gcggggcggc tgggggcggt gacgatcgcc accaacatgg ccggccgcgg
     1501 caccgacatc ctgctcggcg gcaacgccga gttcgtggcg cgccggcagc tcctcgaagg
     1561 gcgcgagccg ggaaacgtcc cgcccccgga ggaactggcc gccgccatcg aggaggcccg
     1621 gcaggcctgc ctgacggagc acgacgaggt ggtcggtctc ggcgggctgc acatcctcgg
     1681 caccgagcgc cacgaggcgc gccgcatcga cagccagctg cgtggccgcg ccggccgcca
     1741 gggggatccg gggtcttcgc gcttctacct gtcgctcgag gacgacctga tgcgcatctt
     1801 cgggtcggac cgcatctccg gcttgatgca gcggctcggc atggaggaag gggtcccgat
     1861 cgagcaccgc atggtgacca atgccatcga gcgcgcgcag aagcaggtcg aggcgcgcaa
     1921 cttcgaggtg cgcaagcacc tcctcgagta cgacgacgtc aacaacaagc agcgcaccga
     1981 gatctaccgc ctgcgccgcg agctgctcga ggggaaaggg cagaaggaat acatcctcac
     2041 caaggcggag gagatcctcg atcacatcat cgactccacc tgcaacccgc aggccgaccc
     2101 ggacgagtgg gagccggaga acctgcgcaa cggcatgctg cgctacttcg gcgtggacac
     2161 gaaccaggca gggatc
//
LOCUS       DASRIQ010000250         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3771455, whole genome shotgun sequence.
ACCESSION   DASRIQ010000250 DASRIQ010000000
VERSION     DASRIQ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771455"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFD06_06180"
     CDS             <1..374
                     /locus_tag="VFD06_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HZN03159.1"
                     /translation="CHSKTRDLERIASEADILIAAIGQPAFVRGEHIRQGAVVVDVGI
                     NRVTDLALVKDAYGPESPRVAQVQDRGFTLMGDVHWRQALEKASWITPVPGGIGPLTI
                     ACLLRNTVRAARLRREPAAWA"
     gene            371..1039
                     /gene="coaE"
                     /locus_tag="VFD06_06185"
     CDS             371..1039
                     /gene="coaE"
                     /locus_tag="VFD06_06185"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZN03160.1"
                     /translation="MSAAPPGILRVGLTGGIATGKTTVARHFAGWGATVIDADRLARE
                     LVEPGAAGYAPVVQEFGPGIVAPDGRIDRAALARIVFADPARRARLEAILHPLIFAAE
                     EAALDRLAASPPEIVAGNAGIAVVNAALLVEAGTWRNYQRLVVVHCDEAVQVGRLMQR
                     DGMGRDDAQARVRAQMPTREKLKLAHYAIDTSGSEAETEARTRDVFAALLRDRTAFRA
                     NPAG"
     gene            complement(1017..1871)
                     /gene="panC"
                     /locus_tag="VFD06_06190"
     CDS             complement(1017..1871)
                     /gene="panC"
                     /locus_tag="VFD06_06190"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZN03161.1"
                     /translation="MAFHRTARDMREASEAIRRQGLRLGLVPTMGALHEGHLSLVAQA
                     RADCDVVVASVYVNPLQFGPREDFAAYPRDLERDARLLGGAGTAILFSPAEGEVHVPD
                     HRTSIEVTGMQDVLCGRSRPGHFRGVCTVVAKLFQIVAPHAAYFGEKDAQQLRIVRRM
                     TRDLHFDMTIVGCPTVRAEDGLALSSRNAYLSPEERHAAPVLHRALQAAAARVRAGER
                     DVVRLVEETRAVIAQEPLARIDYVEAVDDETLGPVEVLDRPVLLAVAVFFGRARLIDN
                     IVIRPDSP"
     gene            complement(1889..>2369)
                     /locus_tag="VFD06_06195"
     CDS             complement(1889..>2369)
                     /locus_tag="VFD06_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZN03162.1"
                     /translation="QRRAPLVQALVDAEIPVMGHIGLTPQSVHVMGGYKVQGKRIDEA
                     HALVEDAQELEAAGAFSIVLEGMPEPVGRRVTESVGVPTIGIGAGRSCDGQVLVFHDL
                     VGLTQGPAPRFVRRYADLAGAISDATKRFIDDVRSGSFPSEAETYSMPAAVGTPPRR"
BASE COUNT          335 a          885 c          852 g          297 t
ORIGIN      
        1 tctgccactc gaagacgcgc gacctcgaga ggatcgcgag cgaggccgac atcctcatcg
       61 ccgccatcgg ccagccggcg ttcgtgcgcg gtgagcacat ccgccagggg gccgtcgtcg
      121 tcgacgtcgg gatcaaccgc gtcaccgacc tggcgctggt gaaggacgcc tacgggcccg
      181 agtcaccccg ggtcgcgcag gtgcaggatc gcgggttcac cctgatgggg gacgtccact
      241 ggcgccaggc gctcgagaag gcctcgtgga tcactccggt gccgggcggc atcgggccac
      301 tcaccatcgc ctgcctgctg cgcaacacgg tgcgcgccgc gcgcctccgc cgcgagcccg
      361 ccgcctgggc atgagcgccg ccccccccgg gatcctccgg gtgggcctca cgggagggat
      421 cgccacaggc aagacgaccg tcgcccgcca cttcgccggc tggggggcca ccgtcatcga
      481 cgccgatcgg ctggcgcgcg agctggtcga gcccggggcc gccggatacg cgcccgtggt
      541 gcaggagttc ggccccggca tcgtcgcccc ggacggccgg atcgaccggg ccgccctcgc
      601 ccgcatcgtc ttcgccgatc cggcgcgccg cgcccgcctc gaagcgatcc tgcacccgct
      661 gatcttcgcc gccgaggagg cggcgctcga ccggctggcc gcgtccccgc cggagatcgt
      721 cgcggggaac gccgggatcg cggtggtgaa cgccgcgctg ctggtggaag ccggcacctg
      781 gaggaactac cagcggctcg tcgtcgtcca ctgcgacgag gcggtccagg tgggacgcct
      841 gatgcagcgc gacggcatgg gccgcgacga cgcccaggcc cgggtgcgtg cccagatgcc
      901 gacgcgcgag aagctgaagc tcgcccacta cgccatcgac accagcggca gcgaggcgga
      961 gacggaggcg cgcacgcgcg acgtcttcgc ggcgctgctg cgcgatcgga ccgccttcag
     1021 ggcgaatccg gccggatgac gatgttgtcg atgagacggg cgcggccgaa gaagacggcg
     1081 accgcgagca ggaccggtcg gtcgagcacc tcgacaggac cgagcgtctc gtcgtccacc
     1141 gcctcgacgt agtcgatccg cgccaggggc tcctgggcga tcaccgcccg cgtctcctcc
     1201 accaggcgca ccacgtcgcg ctcccccgcc cggacccgcg ccgccgcggc ctgaagcgcc
     1261 cggtggagca ccggggcggc atggcgctcc tccggggaga ggtaagcgtt gcgcgacgag
     1321 agggccaggc cgtcctccgc ccgcaccgtg gggcagccga cgatcgtcat gtcgaagtgc
     1381 aggtcgcgtg tcatccttcg gacgatgcgc agctgctgcg cgtccttctc accgaagtac
     1441 gcggcgtgcg gcgcgacgat ctggaacagc ttggcgacga cggtgcagac cccccggaag
     1501 tgccccgggc ggctgcggcc gcacaggacg tcctgcattc ccgtcacctc gatggaagtg
     1561 cggtgatccg gaacgtggac ctccccctcc gcgggggaga acaggatggc cgttcccgcc
     1621 ccccccagga ggcgcgcatc acgctcgagg tcgcgcgggt aggcggcgaa gtcctcgcgc
     1681 ggcccgaact gcagcgggtt gacgtagacc gaggcgacga ccacgtcgca gtcggcgcgg
     1741 gcctgggcca cgagcgacag gtgcccctcg tgcagcgccc ccatcgtcgg cacgagcccg
     1801 agacggagcc cttggcgccg gatcgcctcc gacgcctcgc gcatgtcgcg cgcggtccgg
     1861 tggaacgcca ggctcagcgg gcctggtctc accgccgcgg cggcgtgccg acggcggccg
     1921 gcatcgagta ggtctccgcc tccgacggga acgagccgga acggacgtcg tcgatgaagc
     1981 gcttcgtcgc gtcggagatg gcgccggcga ggtcggcgta gcggcgcacg aagcgcggcg
     2041 ccggcccttg cgtcagcccg acgagatcgt ggaacaccag cacctggccg tcgcaggagc
     2101 gcccggcgcc gatgccgatg gtcgggacgc cgaccgattc ggtgacccgg cggccgaccg
     2161 gctccggcat cccctccagc acgatcgaga aggcccccgc cgcctcgagc tcctgggcgt
     2221 cctcgacgag cgcgtgcgct tcgtcgatcc gtttcccctg caccttgtag ccgcccatca
     2281 cgtgcaccga ctgcggcgtc aggccgatgt gccccatgac cgggatctcc gcgtcgacga
     2341 gcgcctgcac cagcggcgcg cgccgctgg
//
LOCUS       DASRIQ010000251        18374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2180989, whole genome shotgun sequence.
ACCESSION   DASRIQ010000251 DASRIQ010000000
VERSION     DASRIQ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 18374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18374
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180989"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VFD06_06200"
     CDS             complement(<1..1224)
                     /locus_tag="VFD06_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN03163.1"
                     /translation="MRDLPGEEATLVRPEDGGVPPEAGHVQPVRHLGPERLVAIGLNS
                     VIGGGIFVLPAHAAALVGSTSLPAYVATGLLVLGIGLVLGRLAARFETSGGPYLYIER
                     AYGPLAGFQIGWLFWLARTSAMANLLNGVALYVGALWPAFAAPGPRAAVIAACAAAVI
                     GLDIAGIRQAARATDLFAILKIAPLLVVGTAGLWLVEIPRLIPQAVDPGSFLRAVLLL
                     VYAFTGFETLTVPVEESKEPRRDLPRALLATIGAVCGIYLLVHIAALGALADLATEKA
                     PLATLADLEFGTGGRLVITFLAGLSMAGCSLSSLLGGTRMLYAMSSNRRIPAWIGALH
                     PGRRTPMRASFLTGGIAAIGAILGGYAFLAAISSGNRLLIYAACCLACLWTPKKAAKA
                     VKPAGGLLLPAMTLLT"
     gene            complement(1191..1511)
                     /locus_tag="VFD06_06205"
     CDS             complement(1191..1511)
                     /locus_tag="VFD06_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HZN03164.1"
                     /translation="MIVPRRAETTAAGGLSIVWADGHVSTYDPAPLRLACRCAHCEDE
                     WTGERRITAAAIPEDIALLAYRPVGRYGYQLEWSDGHNTGIYTFERLREMCACETCRA
                     KKRP"
     gene            complement(1579..2058)
                     /locus_tag="VFD06_06210"
                     /pseudo
     CDS             complement(1579..2058)
                     /locus_tag="VFD06_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            complement(2071..2385)
                     /locus_tag="VFD06_06215"
     CDS             complement(2071..2385)
                     /locus_tag="VFD06_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03165.1"
                     /translation="MRRDSGMTWTEMGTLVGLAAVLVVGAETWRETRSLQKTVTERLT
                     AMETRLTQVATKVDTMGSRPQQQEQRGPDPNKVYAIKTEGAPFEGPKAAPVTIAEFSD
                     FQ"
     gene            complement(2457..3446)
                     /locus_tag="VFD06_06220"
     CDS             complement(2457..3446)
                     /locus_tag="VFD06_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZN03166.1"
                     /translation="MSCKVLVVAEQDGGAVRDITWELLGLAHKVAREAGWGEGSVKAL
                     LLGQNLDAAAKGLAERGAAEAIVADSPALANYSCDGSAAVIEAQAQAESPALVLLGHT
                     PNGWDVAPHVAAGLDAPLATECSAVTAPGGKPRFTRKAFNGKFVQEIEVGDAPMSIAT
                     VQRGASQAFSGSTQGTVRKVDPGIDPAKLQARFVEVRKGESGGLDLTQAAIIVSGGRG
                     LGAAEKFSIIRDLAQALGGQVGASRPVTDSGWLPHEHQIGSSGVTVAPKLYVACGISG
                     AIQHLVGMRGSGFIVAINKDADAPIFGVADVGVVGDLFEIVPALTKAVQEARK"
     gene            complement(3475..4266)
                     /locus_tag="VFD06_06225"
     CDS             complement(3475..4266)
                     /locus_tag="VFD06_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZN03167.1"
                     /translation="MKTLVLVKQVPNTDSPFRINAAGTWVDEGNLTYGLNDYDRYALE
                     EALRLKDKGAATEVVALSVGPERAATALRTCLATGADRAIHVKDDRLAGSDPLAIAKV
                     IAAAIKGEEFRLIFAGLQADDDNYTQTPALVARLLGRPCATAVLKAELLDGSIRVERE
                     LENNRVQVIEMTLPAVVAVQTGINEPRYASLKGIMAAKKKEIRTVGLSDIGVDAAQVG
                     GGAARLKTVRLAHPPKGKGAEILKGSADEVAKALVQRIREKTGVL"
     gene            complement(4481..4792)
                     /locus_tag="VFD06_06230"
     CDS             complement(4481..4792)
                     /locus_tag="VFD06_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03168.1"
                     /translation="MSLDHLLREVVVSGRLAEPFTASEATRAVSLQDWPLTKVQNYLA
                     RHCIGNLAATVLLVERVSYGKYRLIYDGPREDDACAVPDNGPEGPARCATTDHPGEHQ
                     S"
     gene            4991..6301
                     /locus_tag="VFD06_06235"
     CDS             4991..6301
                     /locus_tag="VFD06_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03169.1"
                     /translation="MSERDRRHGLAAWQAIERLARDILRGRPGAHLLEGRLPVLDLRL
                     SLALAGRPEDAEAFAVSLAAEIERQIDDAIEEQAAFQPGHAWCHRCEASACEHSRPPS
                     SRHVFLGYAPAGAPRWMEFAQYALEARLEGFERLYAEPPAFITVQMDQSDLYGPLLPA
                     FRSAGRDLLGQLVAGFFPIPARAGEGRGVVALTFQAAVRITSEGRRRIGLNILGTAPH
                     GEDLTLLYERQDDLPWRRAVRWAQAALTTAARDARTPRGQRGGTAPGDGSGGAVAPGG
                     GAGEGALERRVLGILQGLARRLEQDRRGSGRRTAHAGERHAGGDRPTRKAIDDIRAAR
                     ADAFLRDDRSGALVVLGDRGRTHFFTPAGRLMSSVRYSREAIVRKQRQGVWRPIAAAD
                     ARAARERLLSGAAGDDAEPLSEGTGGPLGDAGEPAPPAGSGGST"
     gene            6348..7826
                     /locus_tag="VFD06_06240"
     CDS             6348..7826
                     /locus_tag="VFD06_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN03170.1"
                     /translation="MPRSFVLLASIVTIVIPLGVAVPASAQAPFDDAVFLAAGATPEA
                     VAIGDLDGDGWNDLAVVNFGGDLHVLFNRGDGTFDPAVPYLDLWATSVMDVKVADLDR
                     DGDLDLAVAFFRFQGGVSVVLNRGDGTFDPPIEYDSCYSTQGIAVGDMNGDLHADIVG
                     MSNCFRATVLLNDGSAGLAHLGDFGNGYVPGGISMADLDDDGDSDVVYANQGVSTLTV
                     LFNDGSGTLQPFTQYDSPGSPQEVALGDFDGDGDVDIAAANLYGPDDSAAPDDFSLLR
                     NDGTGRFAPAVKTPLGREPEGLVAADFDLDGDLDVVAANRGGNSVTFRWNAGNGTFPS
                     ATTKTAGSGSDDVAAGDLNGDARPDVVAVNQDGASLAVYRNRKIVTADSDGDGATDAA
                     DCAPTDPASWRLPGAVEDLGLAGGATALLSWSEPIDPGGRPPRYDVLRSDDPADFSSP
                     AFVVADAAGRAAADPAVPAEAFFYRVRARNGCGASGATAVSP"
     gene            7905..8237
                     /locus_tag="VFD06_06245"
     CDS             7905..8237
                     /locus_tag="VFD06_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN03171.1"
                     /translation="MFPRELKKGSTELLILSLLEDRPRYGYEIGKRIELRSKGRLTFR
                     IGSLYPMLSRLEARGLITGRWAEKAHEPRRRYYRLTPSGRRALKEKRSVWKEFIEAVN
                     RIAGIGHA"
     gene            8230..11001
                     /locus_tag="VFD06_06250"
     CDS             8230..11001
                     /locus_tag="VFD06_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN03172.1"
                     /translation="MREWAAYVRARLRLPELESGREAEIVEELAQQLDEAAREALASG
                     ATPEEARTRALDHLTDWGRLRDDLLASERLRVPRVERWRDGAETAAAAGSGAARLFGT
                     LRADVLYGLRQLARSPGFAAVAIVTLALGIGATTAIFSVVRAVLLRPLPFPDPQRLVL
                     VFDAGDAPGRERAQPSPGNFLDWRARSDAFDGMAAWYSSSRTLRDETSAEQVQTVQVS
                     GDFFKVWGLAPALGTIFTPEESPGALYNEGNLFVGGDRIAVLSDAFWRRRFGADPAIV
                     DTPIRLDGKTWRVAGVMPPGFAVPDPLVDIWVPWDLARSYDARRFPDGPPRDYRFLHV
                     AARLRAGQSLEQAEANLKAIAADLEQRFPWPNKGWSVRLEPLRDAMVRNDRPALLVLF
                     AAVGFVLLIACANVAGIQMSRASARIQEMAVRTALGASRGRLLRQLLTESVLLSLAGA
                     AAGLLIASSGLDLLVALAPRAIPRLDETRIDAGVLLFTLLVAVATGLIFGLAPALQGS
                     RTDLVAGLKEGGQGGRAAGPARHRLRALLVVSEVALALVLLAGAGLFTRSFARLVAVD
                     PGFDPRRLLVLRVFLDTDAYGSELKSSQYYRQLLERLGALPGVASVSATTVLPMSEVG
                     IDFNRPYWREGDPDPGGQPAMAGIRMVTPDYFSTMRIPVHEGRAFTEGDRLGGAPVIM
                     VNQEMARRVWPGASPVGRRLVIDYKRSKYVYEVVGVAGDTRYDGLRSRPEPEIFIPHA
                     QNPYLAMNVVLRTAVDPRRLARAAAAEALALDPAQPVHSVTTMDQLLERTLAPDRFSM
                     TLLSLLALVAVVLAGVGIYGLTAQVVGLRTHEIGVRIAVGAQPGQVVAMVLRQSCRLA
                     AAGGAVGLLAALALTRLVGGLLYGVGSADPATFAAVTLVLAAVTIGAAWIPARRVLRI
                     DPIAALRHD"
     gene            11015..11686
                     /locus_tag="VFD06_06255"
     CDS             11015..11686
                     /locus_tag="VFD06_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03173.1"
                     /translation="MTRRTAALLVGSLLAGAALAADPPPIPLSAARAYTALGVKVEET
                     EYRGRRALRVVEPKINEGGGIALANGVTFANGTIEVDVAGLPAAGAVEASRGFIGIAF
                     RVQTDPQRYECLYLRPTNGRADDQLRRNHSTQYVSEPEFPWHRLRKEQPGVYESYVDL
                     EPGAWTRLRIVVDGTRARLYVHDAPQPALIVNDLKLGDTQGGIALWIGQGTEAYFSSL
                     TITPR"
     gene            11722..12978
                     /locus_tag="VFD06_06260"
     CDS             11722..12978
                     /locus_tag="VFD06_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HZN03174.1"
                     /translation="MNSDLYTLASFVASAGGDLLAKAAPFEACIADARAKGYHTYRRV
                     ATAGMDHRVPIVDPETQREREFIMLGSNNYLGLANHPKVIEAAVEATRRFGTGMGGPP
                     LLNGTTTLHRRLEERLAAFKRCEDAIVFPTGYAANLGAIAALVRKGDVVVADRLAHAS
                     IFDGCRLSDGAMHAFRHNSAAHLDLILRNKAAAAPGGRLVAVEGVYSMDGDVPPLPDI
                     LAVATRHGAALLVDEAHATGVMGRTGRGTVEHYGLEGRVDLIMGTLSKTLASVGGFIC
                     GRKDVVTYLRYYARSYFFSASPPPATMAAALAALDLVDQEPWRIERLRENIAYLKAQL
                     RALGFDCLDSRSAILPIMIGDEMTCRKIGRRLHELGIFVNPVAYPAVPKNRTRLRLSV
                     MATHTRDDLDRAIEALALAGREHGLR"
     gene            12975..14090
                     /locus_tag="VFD06_06265"
     CDS             12975..14090
                     /locus_tag="VFD06_06265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN03175.1"
                     /translation="MSAAIAAADWDALVRPGAGPLRHGFLEAATRIELAAFEFRPLVL
                     PGGDRGGFAAHAPAYRYDLDLGLFARGGAARALAAVRSAWRRCLVLRVFEVGCPVALC
                     EPFSVASGTDQAAAVEALACAAIDEAGRAGASLVIVHDFESKAPRAATRTLARLGFEP
                     VPILPNFVLDLDGRFETFDAYLAAMRSPYRRRARLRLEKAAALRPELLSDFGEDAPVL
                     ARLWRAIDDRASEARREILTEPFFRAIDGLAHVRMLALRRPDGSIASFALLYEDGATL
                     RFLYSGFLLEAREEGAYFRLLYEIVRVAIEGRFTRVSLGWTTADPKLDLGARPVPLEA
                     WIHHRRRSHQRAIGRAMRLLLRAEDPRPRHVFREAKS"
     gene            complement(14098..>18374)
                     /locus_tag="VFD06_06270"
     CDS             complement(14098..>18374)
                     /locus_tag="VFD06_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZN03176.1"
                     /translation="VVRARDEWDAAGPASVVVSATTLPAPVFASAPAAIVLSVPAGKE
                     IDGGIALTNAGPGTLDWSAEALPPADAPGGAPQAAASEPEPLAWLSFVPPTGRIGAGA
                     TQALQIHVRSVGLTAGEHRATVVVRSNDPARARVEHALTLHVLDASTLLVAPPAIDFD
                     PVVIGTTALRFFTLMNAGTTALDVLVVESDDAAVIPPSGGLFIPPGLTLTLPVVFAPA
                     TPGAVETQLRVTSLADNAAEIGPIPVRGVGLAPPVPTVTPQAVAVALRAGDRTSIPMA
                     LGNSGGSILTVNIETQLTGDDATAPWIAPRPEALTIPPGETASIDLDVNADRLAPGTY
                     EAFLRLNTNEPGRLPPWTVPVTLAVAAGAHLRIESSEVLLESRASFTTPGETTAHHLD
                     APIEPAGGGTVTLRLEGDYGSRLESARLTVEGRDLGTLRGGDPNGPAAGGDNGGTLPP
                     ECNEVSMSAPIDAVGLAALLADRRVEAEVANGPAVDPVCDTSRHTLQIRYAPRLDRID
                     FGDLLAGSERRRQFGLRNSGSDPMRGTLAIAGEAGFSVFPEEFDLAPGASMTATVTFR
                     APAAGAAARTGPRAPEAAAGRLDLDSDDPDRPHLEIALAGTILPLPEIAASPASVAAT
                     LLEGRVEARPIALTNLGTEPVSLALGIAPGDGTAHPSDCRPEALYAATFNTGEVRERD
                     LATGVERVAVSGLFGPRGVAVSPDGRRLYVTEFNGRLAIADLATPGTPARILLGLSTP
                     FGVTLDPEGQTAWVTGFGSGAVVQVALASGASTPVASGLFGPHGIALDAEGLAAFVTE
                     ETRGSLAQVDLEDGSARLIASGLGGVTGLAFDPAGTEAYASLPGRGTIVAIDLVTGTV
                     RDVVSGLSSPTEVVIDPARRLLYVSEFGAARVVSVDPATGARATVIASIPNPTGLALR
                     VPGICSARFARVAARRIEIPGGATVEAVLGLDSTGLPEGARTAALLAGTRAPFVPLAR
                     VPITLDVVARPRLVLSGQPQAVESLLSYTGSAARTVHPLSLPVPPGTAGRLEITVEGD
                     FGNVREQADVFIDGFLIGSVGVTGQDCVPATRVIDLPLPFLRVVAGNDKAEVALQNSA
                     DVAATCAVNRHRVRLTYDSADPQAGLDLGSLDVGELRTVSLLIRNNGLARLDLIDLRS
                     TDPQCTVTPGPLSVGPGAVRGIVLRCVPVRSGPFAATLRLTSNDPDRPVLDTSITATV
                     SDRPPVAALSAPAAVECDRPLAGRVLLDGRASSDPDSIPGTPGDIAAYEWFLRDVVTG
                     VERVVATGALVEVDLPLGSSRVVLRVTDTAGVTSEAEAVVEVRDTRPPAFEVAAAPAI
                     LWPPDHRMRAVRLTPAASDACDPAPAARLVEATSSEPDDAPGIGDGSTTGDIAAGAGC
                     AVIGLRAERIGGGPGRVYRVVCEGSDHSGNATRAETLVRVPATTPP"
BASE COUNT         2506 a         6636 c         6569 g         2663 t
ORIGIN      
        1 cgtcagcagg gtcatggcgg gcaggagaag tcccccggcc ggtttcacgg cctttgcggc
       61 ctttttcgga gtccagaggc aggcgaggca gcacgcagcg tagatgagga ggcggttccc
      121 ggaggagatc gccgccagga aggcataccc tccaaggatg gccccgatgg ccgcgatccc
      181 tccggtcaga aaggaggccc gcatgggggt ccgccgtccc ggatggagcg ctccgatcca
      241 ggccggaatg cggcggttgg acgacatggc gtagagcatc cgcgtccccc cgagcaggct
      301 cgacaggctg cagccggcca tcgacagccc cgcgaggaag gtgatcacga ggcgtccccc
      361 cgtgccgaac tcgaggtccg ccagcgtcgc gagcggggcc ttctccgtcg cgaggtccgc
      421 gagggcgccg agcgcggcga tgtgcaccag caggtagatg ccgcacacgg ccccgatcgt
      481 cgccagaagc gcccgcggca ggtcccgccg gggctctttc gactcttcca ccgggacggt
      541 gagcgtctcg aaccccgtga aggcgtacac gagcagcagg accgcgcgga ggaaggagcc
      601 gggatcgacc gcttgtggga tgaggcgagg gatctcgacg agccagaggc cggcggtgcc
      661 gacgacgaga agcggcgcga tcttgaggat cgcgaagaga tccgtggcgc gggccgcttg
      721 ccggatgccc gcgatgtcga gcccgatgac cgcggcggcg caggcagcga tgacggcggc
      781 acgggggccg ggggcggcga aggccggcca cagggctccg acgtagaggg cgacaccgtt
      841 gaggaggttc gccatggccg acgtgcgcgc cagccagaag agccacccga tctggaatcc
      901 ggcgagcggt ccgtaggccc gctcgatgta gaggtacggc ccgcccgagg tctcgaagcg
      961 ggccgccaga cggccgagga ccaggccaat cccgaggacc agcagaccgg tcgcgacgta
     1021 cgcggggagg ctcgtcgatc ccacgagcgc cgcggcgtgg gccggcagga cgaaaatgcc
     1081 gccgccgatg accgagttca ggccgatcgc gaccagccgc tccggcccga ggtggcggac
     1141 cggctggacg tgacctgctt cgggagggac gcccccgtct tccggccgga ctagggtcgc
     1201 ttcttcgccc ggcaggtctc gcacgcgcac atctcccgca ggcgttcgaa ggtgtagatg
     1261 ccggtgttgt ggccgtcgct ccactcgagc tggtagccgt accggccgac cggcctgtag
     1321 gcgaggagcg cgatgtcttc cgggatcgcg gcggcagtga tccgccgctc gccggtccat
     1381 tcgtcttcgc agtgggcgca gcggcaggcg aggcggagcg gagccggatc gtacgtcgag
     1441 acgtgcccgt cggcccagac gatcgacagg ccgccggcgg cggtggtctc cgcgcgccgc
     1501 gggacgatca tgcgtcggcc ccgcgggggc ggggcgcggc gttcatgcag attcggagaa
     1561 gtgctgtgcc gcgccccgtc acttccccgg cgcgccggcg ggggcctgtg cctgctgcgc
     1621 cgccggcggg atcgggatgt tctgcttctg caactcggcg ttgatcaccg tcgcgaactc
     1681 ggcgaacggc ttcgctccgg acaggaagcg gccgttgacg aagaaggccg gggtgccggt
     1741 gacgccgaga gtcttcgcct cggcgatgtc ggcgtcgatg accgccttcc ccttcgccgt
     1801 gtcgagcgcc tgcttgaagc gctgcacgtc gaggccgagg tccttggcgt actgctcgta
     1861 ctgcgcccgg tccatcttgt cctgcgctcc gaagatcttg tcgtgcatcg gccagaactt
     1921 cccctgttcg gcggcagccg ccgtggccag gtgcgccatc ggcgccttgg gatggaaggg
     1981 gagcgggaag tgcttccaga cgatgcggac cttgtcgccg tatgtcttct gaatctggtc
     2041 gagggtcgga ccgaccctcg cacagtaggg tcactggaag tcggagaact cggcgatggt
     2101 gaccggcgcc gccttcggcc cctcgaacgg cgccccttcg gtcttgatgg cgtagacctt
     2161 gttggggtcg gggccgcgct gctcctgctg ctggggtcgc gaccccatgg tgtccacctt
     2221 ggtggccacc tgggtgaggc gggtctccat ggcggtcagg cgttcggtca cggtcttctg
     2281 gagggaacgg gtctccctcc aggtctcggc gccgacaacc agcaccgccg caagaccgac
     2341 gagcgtgccc atctcggtcc aggtcattcc ggagtcgcgc cgcatgggcg gcccctcctt
     2401 cagattcggt tgtttcgaca tcatgtcgga atccctcttc ggatgggcat tctagactac
     2461 ttgcgggctt cctggaccgc cttcgtcagg gccggcacga tctcgaagag gtcccccacc
     2521 accccgacgt cggccacgcc gaagatcggc gcgtcggcgt ccttgttgat ggcgacgatg
     2581 aagccggagc cgcgcatccc gaccaggtgc tggatcgccc cggagatgcc gcaggcgacg
     2641 tagagcttcg gggccacggt gacgccgctc gagccgatct ggtgctcgtg cggcagccat
     2701 ccggaatccg tgaccggccg tgacgcgccg acctgcccgc cgagcgcctg cgccaggtca
     2761 cggatgatgg agaacttctc cgccgccccg agcccccggc cgccggagac gatgatcgcc
     2821 gcctgggtca ggtcgagccc gcccgactcc cccttgcgca cctccacgaa gcgggcctgg
     2881 agcttcgcgg ggtcgatgcc gggatcgacc ttgcgcaccg tcccctgcgt cgagccggag
     2941 aacgcctgcg aagcgccgcg ctgcaccgtg gcgatcgaca tcggcgcgtc gcccacttcg
     3001 atctcctgga cgaacttgcc gttgaacgcc ttgcgcgtga agcgcggctt cccgcccggt
     3061 gccgtgacgg cggagcactc ggtcgcgagc ggcgcgtcga ggccggcggc gacgtgcggc
     3121 gcgacgtccc agccgttcgg cgtgtgaccg aggaggacga gcgcgggtga ctccgcctgc
     3181 gcctgcgcct cgatgacggc ggcagagccg tcgcagctgt agttcgcgag ggcgggcgag
     3241 tcggcgacga tcgcctccgc cgcaccgcgc tccgccagcc ccttcgcggc ggcgtcgagg
     3301 ttctgtccca ggaggagcgc cttgaccgac ccttcgcccc accccgcctc cctggcgacc
     3361 ttgtgggcga gaccgaggag ctcccaggtg atgtcgcgga ccgctccccc atcctgttcg
     3421 gcgacgacca ggaccttgca actcatgttc tggcgacctc ctggcgcgcg cggctcacag
     3481 gacccccgtc ttctcgcgga tccgctggac cagcgccttc gcgacttcgt cggcggagcc
     3541 cttcaggatc tccgccccct tccctttcgg cggatgggcg aggcggaccg tcttcaggcg
     3601 cgccgctccg ccgccgacct gcgcggcgtc caccccgatg tcggacagcc cgaccgttcg
     3661 aatctccttc ttcttcgccg ccatgatccc cttgaggctg gcgtagcgcg gctcgttgat
     3721 gccggtctgc accgccacga ccgcgggcag cgtcatctcg atcacctgga cgcgattgtt
     3781 ctccagctcc cgctccacgc ggatggaccc gtcgagcagc tccgccttca ggaccgcggt
     3841 ggcgcagggg cggccgagga ggcgcgccac gagcgccggg gtctgcgtgt agttgtcgtc
     3901 gtccgcctgc agccccgcga agatgagccg gaactcctcc cccttgatcg cggcggcgat
     3961 gaccttggcg atcgccagcg gatcggatcc ggcgagccgg tcatccttga cgtggatggc
     4021 gcggtcggcg ccggtcgcga ggcaggtgcg gagcgccgtg gcggcccgct ccgggccgac
     4081 cgagagggcc acgacctccg tcgccgcgcc cttgtccttg aggcgcagcg cctcctcgag
     4141 cgcgtagcga tcgtagtcgt tgaggccgta ggtgaggttc ccttcgtcca cccatgtgcc
     4201 ggcggcgttg atgcggaacg gcgaatcggt gttcgggacc tgcttgacga gaacgagcgt
     4261 cttcatgcgg ctccgtcacg cggatgcgcg gcttgatctc gaaatggatg ttcgtgctat
     4321 cggtcgcgcg aagccggcat cttacaacag agaagtgccc ggggccggtg acacaaatgc
     4381 ccggggccgg actgccttgt cccttgggca gccggctccc ggggtcgtgc gacagcggaa
     4441 aggacggcga cgcgggccgc cctctgggcg gacaggcggg tcaggactgg tgttcgcccg
     4501 gatggtccgt tgtcgcgcag cgtgcggggc cttccggccc gttgtcgggg acggcgcacg
     4561 cgtcgtcttc gcgcgggccg tcgtagatca ggcggtactt gccataggag acccgctcga
     4621 ccagaagcac cgtcgcggcc aggttgccga tacagtggcg ggccaggtag ttctggactt
     4681 tggtcagcgg ccagtcctgc aggctgacgg cacgggtggc ctcggaggct gtgaacggct
     4741 cggcgaggcg tccactgacg acgacttccc tcagaagatg gtcgagactc attttcgacc
     4801 tcctttgcga agaacccggt actcggagca acagcaacga cgacgccggg aatgtacacc
     4861 cggtcacgtt atgctgcaat aacatttaca gggaaagttt tatgcggcac ctgatggcgg
     4921 cgtccacatt tccggatgca aaccccgctc tctccttggc ttcgcgcctc ctgtgcgatc
     4981 atcgagcccc atgagcgagc gtgatcggcg tcacggactg gccgcctggc aggcgatcga
     5041 gcggctggcg cgcgacatcc tgcgcggccg ccccggcgcg cacctgctcg aagggcggct
     5101 cccggtgctc gatcttcgac tgtcgctggc gctcgccggg cgaccggagg acgccgaggc
     5161 gttcgcggtg tcgctcgccg ccgagatcga gcggcagatc gacgacgcca tcgaggagca
     5221 ggcggccttc cagccggggc acgcctggtg ccatcgctgc gaggcctcgg cctgcgagca
     5281 ctcgcgcccg ccgtcgtcgc gccacgtctt cctcggctac gcgccggccg gcgcgccgcg
     5341 ctggatggaa ttcgcgcagt acgccctcga ggcgcgcctc gagggtttcg agcgcctgta
     5401 cgccgagccg cccgctttca tcaccgtgca gatggaccag agcgatctct acggtccgct
     5461 gctgccggcc ttccgcagcg ccgggcgcga ccttctgggg cagctggtcg ccggcttctt
     5521 cccgatccct gcgcgtgccg gagaaggacg cggcgtcgtg gcgctgacct tccaggccgc
     5581 ggtgcgcatc acttccgagg ggcgacggcg catcgggctg aacatcctcg gcaccgcgcc
     5641 gcacggcgag gacctgacgc tgctctacga gcgccaggac gacctgccgt ggcgccgggc
     5701 cgtgcgatgg gcgcaggcgg ccctcaccac cgcggcgcgc gatgcccgga cgccgcgcgg
     5761 acagcgcggc ggcacggccc cgggcgatgg gtcgggcgga gcagttgccc ccggtggagg
     5821 ggccggcgag ggggcgctcg agcgccgggt gctcgggatc ctgcagggcc tcgcgcggcg
     5881 tctcgaacag gaccgccgcg gcagcggccg gcgcacggcg cacgccggcg agcgccacgc
     5941 cggaggggat cgcccgaccc ggaaagcgat cgacgacatc cgcgccgccc gcgcggacgc
     6001 cttcctgcgc gacgatcgca gcggcgccct cgtcgtcctc ggcgatcgcg gccgcactca
     6061 cttcttcacg ccggctggcc ggttgatgtc gagcgtccgt tacagccgcg aggcgatcgt
     6121 tcgcaagcaa cggcagggag tgtggcgccc gatcgccgcg gctgacgcgc gggccgcccg
     6181 cgagcggctg ctgagcggcg ccgccgggga cgacgcggag cctctgtcgg aaggaaccgg
     6241 cgggccgctc ggtgatgccg gcgagccggc gccgccggcc ggcagtgggg gatcgaccta
     6301 ggagcctgtc cggccgccgg gagtatcatc gggcggaggt gagtgccatg ccccgctcct
     6361 tcgtcctcct cgcgtcgatc gtcaccatcg tcatcccgct cggagtcgcc gtcccggcat
     6421 cggcccaggc gcctttcgac gacgccgtct tccttgccgc gggcgcgacg ccggaagccg
     6481 tggcgatcgg cgatctcgac ggcgacggct ggaacgacct cgccgtggtc aacttcggcg
     6541 gcgacctgca cgtcctgttc aaccgaggcg acggtacgtt cgaccccgcc gttccgtacc
     6601 tcgacctgtg ggccacctcg gtgatggacg tcaaggtcgc cgacctcgac cgggatggag
     6661 acctcgatct ggccgtcgcg ttcttccgct tccagggggg tgtctcggtc gtgctcaacc
     6721 gcggcgacgg caccttcgat ccgccgatcg aatacgactc ctgctactcc acccagggga
     6781 tcgcggtcgg cgacatgaac ggcgacctcc acgcggacat cgtcggcatg agcaactgct
     6841 tcagggcgac ggtgctcctg aacgacggct ccgcgggcct ggcgcacctc ggcgatttcg
     6901 gcaacgggta cgttcccggc gggatttcca tggccgacct cgacgacgac ggggacagcg
     6961 acgtcgtcta cgccaatcag ggagtctcga cactgaccgt cctgttcaac gacggatcgg
     7021 ggacgctgca gccgttcacg cagtacgact cgcccggcag cccgcaggag gtggcgctcg
     7081 gcgatttcga cggcgacggc gacgtggaca tcgccgccgc gaatctttac ggccccgacg
     7141 attccgcggc gccggatgac ttttctctcc tgaggaacga cggcaccggc cggttcgcgc
     7201 cggcggtcaa gacgccgctc ggccgcgagc ccgagggact ggtcgcggcg gatttcgatc
     7261 tcgacgggga tctcgacgtc gtggcggcca accgcggcgg caacagcgtg acgttccgct
     7321 ggaacgccgg gaacggcacg ttcccctcgg ccaccacgaa gacggcggga agcgggtcgg
     7381 acgacgtggc cgccggagac ctcaacggcg acgcccgccc cgatgtcgtc gccgtcaacc
     7441 aggacggggc cagcctcgcc gtctatcgca accggaagat cgtcaccgcc gactccgacg
     7501 gcgacggggc gaccgatgcc gccgactgcg ctccgaccga ccccgcctcc tggcgtctgc
     7561 cgggagccgt ggaggatctc ggcctcgccg gcggggcgac agccctcctg tcatggagcg
     7621 agccgatcga cccgggcggc cggcctccac gctacgacgt gctgcgcagc gacgacccgg
     7681 ccgatttttc ttcgccggcg ttcgtcgtgg cggacgcggc gggtcgcgcg gcggccgacc
     7741 ccgccgtccc ggcggaggcc ttcttctacc gcgtacgcgc ccggaacggc tgcggcgcgt
     7801 cgggcgccac ggccgtttcc ccttagcgcc ccgcccgcca tccggaaccc cgaccaccca
     7861 tatatcgtat tgaagtatgc tgtggcgccg catagggggc ccggatgttt ccgcgcgaac
     7921 tcaagaaggg cagcaccgag ctcctgattc tctccctgct cgaggatcgc ccccgctacg
     7981 ggtacgagat cggcaagcgg atcgagctgc ggtcgaaggg ccggctgacg ttccgcatcg
     8041 gctccctcta cccgatgctc agccggctcg aggcgcgcgg gctcatcacc gggcgctggg
     8101 cggagaaggc gcacgagccg cggcggcgct attaccggct gacgccgtcc gggcgccggg
     8161 ccctcaaaga gaagcggagc gtctggaagg agttcatcga agcggtcaac cggatcgcgg
     8221 ggatcggcca tgcgtgagtg ggcggcgtac gtccgcgcgc gcctgcgtct gccggagctg
     8281 gagtcggggc gcgaagcgga gatcgtcgag gagctggcgc agcagctcga cgaggcggcg
     8341 cgcgaagcgc tggcctcggg cgccacgccg gaggaggcgc gcacccgcgc cctcgaccac
     8401 ctgacggact ggggccggct gcgggacgat ctcctcgcct cggagcgcct cagggtgccg
     8461 cgggtggagc gctggcgcga cggcgcggaa accgcggcgg ccgcgggctc gggcgccgcc
     8521 cggctcttcg gaacgctgcg cgcggacgtg ctgtacgggc tgcgccagct cgcccggagt
     8581 cccggcttcg cggcggtcgc catcgtgacg ctggccctgg gcatcggggc gaccacggcg
     8641 atcttcagcg tcgtccgcgc ggttctgctg cgcccgctcc ccttccccga cccccagcgc
     8701 ctggtgctcg tgttcgatgc cggcgacgct ccggggcggg agcgcgccca gccgtctccc
     8761 gggaacttcc tcgactggcg ggcgcgcagc gatgccttcg acggcatggc ggcgtggtat
     8821 tcgtcctcgc gcacgctgcg cgacgagacc agcgccgagc aggtccagac ggtgcaggtg
     8881 tcgggggact tcttcaaggt gtggggtttg gcacccgcgc tcggcacgat cttcaccccc
     8941 gaggagtcgc cgggcgccct ctacaacgaa ggcaacctct tcgtcggcgg ggatcgcatc
     9001 gcggtgctga gcgacgcctt ctggcggcgg cgcttcggag ccgatcccgc catcgtcgac
     9061 acgccgatcc ggctcgacgg caagacctgg cgcgtcgcgg gggtgatgcc gccgggcttc
     9121 gccgttcccg atccgttggt tgacatctgg gtgccctggg acctggcgcg gtcgtacgat
     9181 gcgcgccgct tccccgatgg accgccgcgc gactatcgct tcctgcacgt ggcggcccgc
     9241 ctccgggccg ggcagtcgct cgagcaggcg gaggccaatc tcaaggcgat cgcggccgac
     9301 ctggagcagc gcttcccctg gccgaacaag ggatggagcg tgcggctgga gccgctgcgc
     9361 gacgcgatgg tgcgcaacga tcgtccggcc ctgctcgtgc tgttcgccgc ggtcggattc
     9421 gtgctcctga tcgcctgcgc caatgtcgcc ggcattcaga tgtcacgcgc ctcggcgcgc
     9481 atccaggaga tggccgtccg cacggccctg ggcgcctcgc gcgggcgcct gctgcgccag
     9541 ctcctgaccg agagcgtgct cctgtcgctc gccggcgccg cggccggtct gctgatcgcg
     9601 tcctcggggc tcgatctcct ggtggcgctc gccccgcgcg ccatcccgcg cctcgacgag
     9661 acccgcatcg acgccggcgt gctgctgttc acgctgctgg tcgcggtcgc gaccggcctc
     9721 atcttcggcc tggccccggc gctccagggc agccggaccg acctggtcgc cgggctcaag
     9781 gagggagggc agggcggccg cgcggcgggg cccgcgcgtc atcgcctgcg cgcgctgctc
     9841 gtggtctccg aggtcgcgct ggcgctcgtg ctcctggccg gcgccggcct gttcacccgg
     9901 agtttcgcgc gcctggtcgc ggtcgatccc gggttcgacc cgcgtcgtct cctggtgctg
     9961 cgggtcttcc tcgacaccga cgcctatggc agcgagttga agtcgtcgca gtattaccgg
    10021 cagctcctgg agcggctggg agcgcttccg ggagtcgcct cggtctcggc gacgaccgtc
    10081 ctgccgatga gcgaagtggg gatcgatttc aatcgcccct actggcgcga gggggatccc
    10141 gaccccggcg ggcagccggc catggcgggc atccgcatgg tcacgccgga ctacttctcc
    10201 accatgcgca tccccgtgca cgaggggcgg gctttcactg aaggcgatcg cctcggcggc
    10261 gcgcccgtca tcatggtcaa ccaggagatg gcgcgccggg tctggcccgg ggcgtcgccc
    10321 gtcggccggc ggctggtgat cgactacaag cgcagcaagt acgtctacga agtcgtgggc
    10381 gtcgcgggcg acacccggta cgacgggctc cggagccggc ccgagcccga gattttcatc
    10441 ccgcacgcgc agaaccctta tctcgccatg aacgttgttc tccgtacggc ggtcgacccg
    10501 cggcgcctcg cgcgggcggc ggcggccgaa gcgttggcgc tcgatccggc gcagccggtg
    10561 cacagcgtca cgacgatgga tcagctcctg gagcgcacct tggcgccgga tcgattctcg
    10621 atgactctgc tctcgctcct cgctctcgtc gccgtggtgc tggcgggcgt cggcatctac
    10681 ggcctgaccg cccaggtcgt cggcctgcgg acgcacgaga tcggcgtgcg catcgccgtc
    10741 ggggcgcagc cgggccaggt cgtcgcaatg gtgctccggc agtcgtgccg cctggcggcg
    10801 gcgggggggg cggtgggcct cctggcggct ctggccctga cgcgtctcgt cggcggcctg
    10861 ctgtacggcg tcggcagcgc cgatcccgcg acattcgcgg cggtcaccct ggtcctcgcc
    10921 gccgtgacga tcggcgccgc ctggattccg gcccggaggg tcctgcgcat cgacccgatc
    10981 gcggcgctcc ggcatgacta gacaacgctc cgccatgacg agacggacgg ccgcgctcct
    11041 cgtgggttcg cttctggcgg gcgcggccct cgcggcagac ccgccgccga ttccgctctc
    11101 cgccgcccgg gcctacacgg ccctcggcgt caaggtcgag gagaccgagt accggggccg
    11161 gcgggcgctg cgggtcgtgg agcccaagat caacgaaggc ggcggcatcg cgctcgcgaa
    11221 cggcgtgacc ttcgccaacg ggacgatcga ggtggacgtc gctggattgc cggccgcggg
    11281 ggcggtcgaa gcgtcgcgcg gtttcatcgg catcgccttc cgggtacaga ccgatccgca
    11341 gcgctacgag tgcctctacc tgaggcccac caacggccgc gccgacgacc agctgcggcg
    11401 caaccactcg acgcagtacg tctccgaacc cgagttcccg tggcaccggc tgcgcaagga
    11461 gcagccgggc gtctacgagt cgtacgtcga cctcgagccc ggcgcctgga cgcgcctccg
    11521 catcgtcgtg gacggcaccc gcgcccggct gtacgtccac gatgcgccgc agccggcctt
    11581 gatcgtcaac gacctgaagc tcggggacac ccagggcggg atcgccctct ggatcggcca
    11641 ggggaccgag gcgtacttct cgtccctgac gatcaccccc aggtaggaaa ccgcgggccg
    11701 ccggacggcg tagcaccgtc cgtgaactcc gacctctaca ccctcgcgag cttcgtcgcg
    11761 tcggcgggcg gggatctgct ggcgaaagcc gcccccttcg aggcctgcat cgccgacgcc
    11821 cgcgccaagg gctaccacac ctaccggcgc gtcgcgaccg ccggcatgga ccaccgggtc
    11881 ccgatcgtcg atccggagac ccagcgcgag cgcgagttca tcatgctcgg ctcgaacaac
    11941 tacctcggac tcgcgaacca tcccaaggtc atcgaggcgg cggtcgaggc gacgcgccgc
    12001 ttcggaacgg gcatgggcgg acccccactc ctcaacggga cgacgacgct tcatcgccgg
    12061 ctcgaggagc ggctcgcggc gttcaagcgc tgcgaggacg cgatcgtctt cccgaccggc
    12121 tatgccgcga acctcggcgc gatcgccgcc ctggtccgca agggggacgt ggtcgtcgcc
    12181 gaccggctcg cgcacgcctc gattttcgac ggctgccggc tctccgacgg cgcgatgcac
    12241 gccttccgcc acaacagcgc ggcgcacctg gacctcatcc tccgcaacaa ggcggcggcc
    12301 gcgcccggcg gacgactcgt cgcggtcgag ggcgtgtaca gcatggacgg cgacgtcccg
    12361 ccgctccccg acatcctcgc ggtcgcgaca cggcacggcg ccgcgctcct tgtggacgag
    12421 gcgcacgcga ccggcgtcat ggggaggacc ggccgcggca cggtcgagca ctacggcctc
    12481 gagggccgcg tggacctcat catggggaca ctctcgaaga cgctcgcgag cgtgggcgga
    12541 ttcatctgcg ggcgcaagga cgtcgtgacg tacctgcgct actacgcgcg ctcatacttc
    12601 ttctcggcga gcccgccccc cgcgacgatg gcggcggcgc tcgcggcgct cgatctcgtg
    12661 gatcaggaac cgtggaggat cgagcgcctc cgggagaaca tcgcgtacct caaggcgcag
    12721 ctccgcgcgc tcggcttcga ctgcctcgac agccgatcgg ccatccttcc gatcatgatt
    12781 ggcgacgaga tgacctgccg gaagatcggc cgccgcctcc acgagctcgg catcttcgtg
    12841 aatccggtcg cctatccggc cgtcccgaag aaccgcacgc gcctccgcct gagcgtcatg
    12901 gcgacgcaca cgcgcgacga cctcgaccgc gccatcgagg cgctcgctct ggccggacgc
    12961 gagcacggcc tccggtgagc gcggcgatcg cggcggccga ctgggacgcc ctcgtccggc
    13021 cgggcgcggg accgctgcgc cacggtttcc tcgaggcggc gacccggatc gagctcgccg
    13081 cgttcgagtt ccgtccgctc gtgcttcccg gcggcgaccg cggcgggttc gccgcgcacg
    13141 cccccgccta ccgctacgat ctcgacctcg gactcttcgc gagaggcggc gccgcgcggg
    13201 cgctcgcggc cgtccgctcg gcctggcgcc gctgtctcgt gctccgcgtc ttcgaggtcg
    13261 gctgccccgt ggcgctctgc gaaccgttca gcgtcgcatc cggcacggac caggccgcgg
    13321 ccgtggaggc gctcgcgtgc gccgccatcg acgaggcggg ccgcgccggt gcttcgctcg
    13381 tcatcgtcca cgacttcgaa tcgaaggcgc cgcgggccgc cacccggacc ctcgcccggc
    13441 tcggcttcga gccggttccg atcctcccga acttcgtcct cgacctcgac ggacgcttcg
    13501 agaccttcga cgcctatctc gccgccatgc gctcgcccta ccggcgtcgc gcgcgcctgc
    13561 gcctcgaaaa ggcggcggcg ctccggccgg agctcctgtc cgatttcggc gaggacgcgc
    13621 ccgtgctggc tcgcctgtgg cgcgcgatcg acgaccgcgc gtccgaggcc cggcgcgaga
    13681 tcctgaccga gccgttcttc cgggcgatcg acgggctcgc gcacgtccgc atgctcgcgc
    13741 tccggcgccc cgacggctcg atcgcctcgt tcgcgctcct gtacgaggac ggggcgacgc
    13801 tccgcttcct ttactcgggc ttcctcctcg aagcgcggga ggaaggggcc tatttccgtc
    13861 tcctctacga gatcgtccgc gtcgcgatcg aggggcgctt cacccgggtg agcctcggct
    13921 ggacgaccgc cgatccgaag ctcgacctcg gcgcgagacc tgtcccgctc gaagcctgga
    13981 tccatcaccg ccgccgatcg caccagcgcg cgatcggccg ggcgatgcgg ctgcttcttc
    14041 gagcggagga ccccaggcct cgccacgtgt tcagggaggc gaagtcgtag cccctgccta
    14101 ggggggtgtc gtggccggga cgcggacgag ggtttcggcg cgcgtcgcgt tgccggagtg
    14161 gtcgctcccc tcgcacacca cgcggtacac acgcccgggg ccgccgccga tccgctccgc
    14221 gcgcagcccg atcaccgcgc agccggcccc cgctgcgatg tcccccgtgg tgctgccgtc
    14281 gccgatgccg ggcgcgtcat ccggctcgct gctcgttgcc tcgacgaggc gcgcggccgg
    14341 cgccgggtcg cacgcgtcgc tcgcagccgg cgtgagacgc accgcccgca tcctgtggtc
    14401 gggaggccag aggatggccg gagcggccgc cacctcgaac gccggcggcc gcgtgtcgcg
    14461 cacctcgacg accgcctccg cctcggacgt cacccccgcc gtgtccgtga cgcgcaggac
    14521 gacgcgcgac gaaccgagag gcaggtcgac ctcgacgagc gcgccggtcg cgacgacgcg
    14581 ctccaccccg gtgacgacgt cgcgcaggaa ccattcgtac gccgcgatgt cgccgggcgt
    14641 ccccgggatc gaatcgggat cgctcgaggc gcgcccgtcg agaagcaccc ggcccgcgag
    14701 cggccgatcg cactcgacgg cggccggcgc ggagagcgcc gcgaccggcg ggcgatcgct
    14761 cacggtcgcg gtgatcgacg tgtccagcac cggccgatcc gggtcgttgc tcgtcaggcg
    14821 cagcgtcgcg gcgaaggggc cgctccggac ggggacgcag cgcaggacga tcccccgcac
    14881 cgcacccggc ccgacggaca gggggccagg cgtgacggtg cactgcggat cggtcgagcg
    14941 gagatcgatg aggtcgaggc gcgccaggcc gttgttgcgg atgaggagcg acaccgtccg
    15001 cagctcgcca acgtcgagcg acccgagatc gagacccgcc tgcgggtcgg cgctgtcgta
    15061 ggtcagccgc acccggtgcc ggttcaccgc gcacgtcgcg gcgacgtcgg cgctgttctg
    15121 cagcgccacc tcggccttgt cgttgccggc gacaacccga aggaagggca gcggcagatc
    15181 gatgacgcgg gtcgcgggaa cgcagtcctg gccggtgacg ccgacggacc cgatgaggaa
    15241 cccgtcgatg aaaacatccg cctgctcgcg cacgttgccg aagtcccctt ccaccgtgat
    15301 ttcgagccgg ccagccgtcc cgggcggcac gggaagggac aacgggtgca cggtgcgcgc
    15361 cgcggagccg gtgtaggaca gcagcgactc gaccgcctgc ggctgccccg acagaacgag
    15421 gcgcgggcgg gccacgacgt cgagcgtgat cggcacgcgc gcgagcggca cgaacggcgc
    15481 ccgcgtcccg gccaacagcg cggcggtgcg cgctccttcc ggcagcccgg tgctgtcgag
    15541 ccccaagacc gcttcgaccg tcgcgccccc aggaatctcg atccgccggg cggcgacgcg
    15601 ggcgaaccgg gccgagcaga tccccggaac acggagcgcc aggccggtcg ggttcgggat
    15661 cgaagcgatg accgtggcgc gcgcgccagt cgccggatcg accgagacga cccgggcggc
    15721 gccgaactcg ctgacataga gcaggcggcg ggccggatcg atcaccacct cggtcgggct
    15781 ggagagcccc gagacgacgt cgcgcacggt cccggtcacg aggtcgatcg ccacgatcgt
    15841 gccgcgtccg gggagggagg cgtacgcctc ggtgcccgcc ggatcgaaag cgagcccggt
    15901 cacgccgccc aggccggacg cgatgagccg cgcgctcccg tcctcgagat cgacctgcgc
    15961 gagcgagccg cgcgtctcct cggtcacgaa ggcggcgagg ccttccgcgt cgagagcgat
    16021 gccgtgcggg ccgaagaggc cggacgcgac cggcgtcgaa gcgcccgacg ccagcgcgac
    16081 ctggacgacg gcgcccgaac cgaacccggt cacccaggcg gtctgcccct ccggatcgag
    16141 ggtcactccg aacggcgtgc tgagaccgag gaggatgcgg gccggggttc ccggcgtcgc
    16201 cagatcggcg atcgccaacc ggccgttgaa ctcggtgacg taaagacgcc ggccgtcggg
    16261 gctcacggcg acgccgcgcg gaccgaacag accgctcacg gcgacccgct cgactccggt
    16321 cgcgagatcg cgctcccgca cctccccggt attgaacgtg gcggcgtaga gcgcctcggg
    16381 gcggcagtcg gacggatgcg ccgtcccgtc gccgggggcg atcccgagcg ccagcgacac
    16441 cggctcggtg ccgaggttcg tcagagcgat cggccgcgcc tcgacccgcc cttcgagcag
    16501 ggtcgccgcg acactggcgg gcgatgccgc gatctccggc agcggcagga tggtcccggc
    16561 gagcgcgatc tcgagatgcg gccggtccgg atcgtcgctg tcgagatcga gccggccggc
    16621 cgccgcctcc ggcgcgcgcg ggccggtccg cgccgcggcg ccggcggccg gcgcgcggaa
    16681 cgtcactgtc gcggtcatcg aggctccggg ggcgagatcg aactcctccg ggaaaacgga
    16741 gaagccggct tcccccgcga tcgcgagcgt gccgcgcatc ggatcgctgc cgctgttgcg
    16801 cagtccgaac tgccggcggc gctccgaccc cgcgaggaga tcgccgaagt cgatccggtc
    16861 gagccgcggc gcgtagcgga tctgcagcgt gtgccggctg gtgtcgcaga ccggatcgac
    16921 ggcggggccg ttggccacct ccgcctcgac ccgccgatcg gcgaggagcg cggcgagccc
    16981 caccgcgtcg atcggggcgc tcatcgacac ctcgttgcac tcgggcggaa gggtgccgcc
    17041 gttgtccccg ccggctgcag ggccgttcgg atcgccgccg cgcagcgtcc cgaggtcgcg
    17101 tccttcgacc gtcagacggg ccgattcgag ccggctgccg tagtccccct cgaggcgcag
    17161 ggtcaccgtg ccgccgccgg ccggctcgat cggggcatcg agatgatgcg cggtcgtctc
    17221 ccccggcgtc gtgaacgacg cccgggactc gaggaggacc tccgacgatt cgatccgcag
    17281 atgcgcgccg gcggcgaccg cgagcgtgac cggcacggtc cagggtggca gacgccccgg
    17341 ctcgttggtg ttcagacgca ggaacgcttc gtatgtcccc ggagcaagcc gatccgcgtt
    17401 gacgtcgaga tcgatggagg cggtttctcc cggtgggatc gtcagggctt ccggcctcgg
    17461 cgcgatccat ggagccgtgg cgtcatcccc cgtcagctgc gtctcgatgt tcacggtgag
    17521 gatcgaccct ccgctgttgc cgagcgccat cgggatcgag gtgcggtccc ccgcgcgcag
    17581 cgcgacggcg accgcttgcg gcgtcaccgt cgggaccggc ggcgccagac cgacgccgcg
    17641 caccgggatc ggcccgatct cggccgcgtt gtcggccagc gacgtcactc gcagttgcgt
    17701 ttcgacggcg ccgggcgtcg ccggcgcgaa caccaccggg agcgtcagcg tcagccccgg
    17761 ggggatgaac aggccccccg acggcgggat caccgccgcg tcgtcgcttt cgaccacgag
    17821 gacgtcgaga gccgtggtcc ccgcgttcat cagggtgaag aaccgcagcg ccgtggtgcc
    17881 gatcaccacc gggtcgaagt cgatcgcggg cggcgcgacg agcagcgtcg aggcgtcgag
    17941 aacgtggagc gtcagcgcat gctcgacgcg cgcccgcgcc ggatcgttgc tgcggacgac
    18001 gaccgtcgcg cggtgttctc ccgccgtgag cccgacgctc cggacgtgga tctgcagcgc
    18061 ctgcgtggcg ccggccccga tcctcccggt cggcggcacg aacgacagcc aggccagcgg
    18121 ctccggctca ctggccgccg cttgcggcgc gccgcccggg gcgtccgcgg gcggcagcgc
    18181 ttcggcgctc cagtcgagcg ttccgggacc ggcattcgtg agcgcgatcc cgccgtcgat
    18241 ctccttcccc gccggaaccg acaggacgat cgcggccggg gcggacgcga acacaggggc
    18301 cggcagcgtc gtcgcagaga cgaccacgga cgcaggaccg gccgcgtccc actcgtcccg
    18361 ggcgcgcacg acga
//
LOCUS       DASRIQ010000252         4901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_631253, whole genome shotgun sequence.
ACCESSION   DASRIQ010000252 DASRIQ010000000
VERSION     DASRIQ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631253"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1000
                     /gene="hpnD"
                     /locus_tag="VFD06_06275"
     CDS             143..1000
                     /gene="hpnD"
                     /locus_tag="VFD06_06275"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HZN03177.1"
                     /translation="MEILTQRSARRGDSPGSTGSSFHYSFTLLSRERRRAIEAVYAFC
                     RVVDDLADEAASSRDAQAGLYLFRDELVRCYDGAPTQPVTRSLQEHIRRYRIPREPFE
                     AILEGVEMDLHKRRYANFAELRGYCVRVASAVGLVCLPIFGAVHPKSRDYAADLGLAL
                     QLTNILRDLKSDAERGRIYLPADEMEGFGYHEKALYRGERSSAFLGLMRYQADRAERH
                     FESAAALLPSSDRRALLPAEVMGAIYRRLLQRMRDERFPVFERRVAVPRLTQVRLTLA
                     AWVTGRAGA"
     gene            1009..2373
                     /gene="hpnE"
                     /locus_tag="VFD06_06280"
     CDS             1009..2373
                     /gene="hpnE"
                     /locus_tag="VFD06_06280"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HZN03178.1"
                     /translation="MAARGGGSPDVVVVGGGFAGLAAATALAQTGAKVLLCEERPHCG
                     GRARSWIDPDTGSIVDNGQHLFAGCYAEMLRFLERIGAADRIAWGSGLEVPYLDPGGK
                     AAVLRAGAGRWRALAGLMRYKGLPFGERMAAARVPLAARRPDEDLDALTVAQWLARLE
                     QSTEARRRLWDPLAIAALNEAPEKAAAGGFAAALRHAFFSGPRGIALGSPRVGLSDLY
                     VEPGIQYLRARGSEVRLRAPVQRLLESGTRCSGVLLHGGARVEAGAVIAAVPPKPLLE
                     ILPPALAAAPFFARAGGLKDSAILSVYLWFGAPVVDRPYAALLGGFWQWAFERDGVRP
                     PPGAEGGPAVHGGMHPVTLVRSAAGAEVDRPKEALVRLALEELRRYFPQAARLTPRHD
                     LVIKEKSATVSLTPGTAALRPPTVTPLDRFYLAGDWIGTGIPATVESACLSGHAAARA
                     AARY"
     gene            2407..2886
                     /locus_tag="VFD06_06285"
     CDS             2407..2886
                     /locus_tag="VFD06_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03179.1"
                     /translation="MAPLLAGAGLCLLAFVVWLVTTHFRERPWFDRPGFARWRGFDAL
                     ASLLRWALFAAGLLALSAHSGAGAAVAVGLFLFGLGWRLAARSAALKRRAMRRALVEL
                     RSKHPGEPETELLVRLVLAAHPRWGEELVRQMALDNPDVDDMARVVARMEQGFRGFR"
     gene            complement(2925..4097)
                     /locus_tag="VFD06_06290"
     CDS             complement(2925..4097)
                     /locus_tag="VFD06_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN03180.1"
                     /translation="MNLETIQASLKELGLPAWLLYDFHGSNPIARHVAGLGPERLVTR
                     RWFGLLPAQGEPTWLHHAIEAHLFTELPGKRIPFVGYRELQERLREILKGIDRVAMEY
                     VPQGAIPYVSRVDGGTIEMVRAAGPTVVSSADLVQYCEARWSAEGLASHRRAAQGLRE
                     VLQATFQEVASRVGGGQEPSEHAIQQFMMEQFRARKLFAEPPIVAVNAHAGNPHYEPR
                     ATGSAPIRRGDLLLLDLWCREEKEGSIWADITWMAFLGSKVPEKHREVWDIVAGARDA
                     AVAFLKESVGRGKPVRGSEVDEVTRDHIRKRGYADRFLHRTGHSIGFEVHGNGCNIDA
                     LETIDDRKLIPRTGFSIEPGIYLADFGIRSEIDVYMDERTVEVTTAVQDQIVPLLT"
     gene            complement(4129..>4901)
                     /locus_tag="VFD06_06295"
     CDS             complement(4129..>4901)
                     /locus_tag="VFD06_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009751372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN03181.1"
                     /translation="DDNIPDVTCGAGQCLSSAPGCINGTPGICVPGTPSPETCNGLDD
                     NCDSLIDNDADTDLDGLFNCTDPDDDNDGVDDAADCAPITHWVSGSPGTVSDTIVADG
                     AVPGKYSWLLLAQTQIYGVYRGIAGPNMPGEYLSSSQCLQPEVPQGTFTDPDVPPVGQ
                     IYYYLVGGYNACGSGSLGTSSNGQPRNITPLCAPSGTDVDGDTIASVYDVCPLVPNPS
                     QADGDRDGRGDVCDNCPAVANPTQADSDHDGLGDACDP"
BASE COUNT          611 a         1681 c         1846 g          763 t
ORIGIN      
        1 ggctgggctg ggagctgcgg ctgacctggc tcggcggcat gcgcatcctc gagcggatcg
       61 aggcggccgg ttacgacgtg ctgtcgcgtc cggtgcgcat cggcgccgcg gatgcggccc
      121 ggatcgcctt gcggagcctc acgtggagat cctgacgcag cggtccgccc ggcgcggcga
      181 ttcccccggg agcaccgggt cgtcgttcca ctattccttc acgcttctgt cgcgcgagcg
      241 gcgccgggcg atcgaggcgg tgtacgcctt ctgccgcgtc gtggacgatc tggcggacga
      301 ggcggcgtcg agccgcgacg cgcaagccgg cctgtatctc ttccgcgacg agctggtgcg
      361 ctgctacgac ggcgccccga cccagccggt cacccgcagc ctgcaggagc acatccggcg
      421 ctaccgcatc ccgcgcgagc cgttcgaggc gatcctcgaa ggggtggaga tggacctgca
      481 caagcggcgc tacgccaact tcgccgagct gcgcggctac tgcgtgcggg tggcgtcggc
      541 cgtcgggctc gtctgcctgc cgatcttcgg agcggtccac ccgaagagcc gcgattacgc
      601 cgccgatctc ggcctggcgc tgcagctcac caacatcctg cgcgacctca agtccgacgc
      661 ggagcgcgga cgcatctacc ttccggccga cgagatggag gggttcggtt atcacgaaaa
      721 ggcgctttac cgcggcgagc gatcgagcgc cttcctcggc ctgatgcgct accaggccga
      781 ccgggccgag cgccacttcg aatcggccgc ggcgctcctg ccgtcgagcg atcggcgggc
      841 cctcctgccg gcggaggtga tgggggcgat ctaccggcgc ctgctgcagc ggatgcgcga
      901 cgagcgcttc ccggtcttcg agcgccgcgt cgccgtgccg cggctcacgc aggtccgcct
      961 gacgctcgcc gcctgggtca ccgggcgcgc cggcgcctga cgcgcggcat ggcggcgcgc
     1021 ggcggcggat cccccgatgt cgtcgtcgtg gggggcgggt tcgccgggct cgccgccgcg
     1081 accgcgctcg cccagaccgg ggcgaaggtg ctgctgtgcg aggagcggcc gcactgcggc
     1141 ggacgggcgc gctcctggat cgatcccgac accggcagca tcgtggacaa cggccagcac
     1201 ctgttcgcgg gctgctacgc ggagatgctg cgcttcctcg agaggatcgg agccgccgat
     1261 cggatcgcct gggggagcgg tctggaagtg ccttatctcg acccgggcgg gaaggcggcg
     1321 gtgctgcgcg ccggagcggg gcgctggagg gcgctcgcgg gcctgatgcg ttacaagggg
     1381 ctgccgttcg gagagcggat ggccgcggcc cgcgttccgc ttgcggcgcg ccgcccggac
     1441 gaggatctcg acgccctgac cgtggcgcaa tggctggcgc gcctggagca gtcgacagag
     1501 gcgcgccggc gtctgtggga tccgctggcg atcgcggcgc tcaacgaggc gccggagaaa
     1561 gcggcggcgg gtgggttcgc cgcggcgctg cggcacgcct tcttcagcgg cccgcgcggc
     1621 attgcactgg ggtcgcctcg cgtcggtctg agcgatctgt acgtcgagcc ggggatccag
     1681 tacctgcgag cccgcggctc ggaggtgcgg ctgcgcgccc ccgtgcagcg cctcctcgaa
     1741 tcggggacgc gctgctcggg ggtgctgctg cacggggggg cgagggtgga agcgggcgcg
     1801 gtgatcgcgg cggtgccgcc gaagccgctc ctcgagatcc tgccgccggc gctcgcggcg
     1861 gccccgttct tcgcccgagc gggggggctc aaggactcgg cgatcctgtc ggtctacctc
     1921 tggttcgggg cgccggtcgt cgatcgcccg tacgcggccc tgctcggcgg cttctggcag
     1981 tgggccttcg agcgggacgg cgttcgtccg ccgcccgggg cggaaggggg gccggcggtg
     2041 cacggcggga tgcaccccgt cacgctcgtg cgcagcgccg ccggcgccga ggtcgatcgc
     2101 cccaaggagg cgctagtgcg cctggcgctc gaggagctgc ggcgctattt cccgcaggcg
     2161 gcgcggctga caccgcgcca cgacctggtc atcaaggaga agagcgccac cgtctcgctc
     2221 acgccgggaa cggcggcgct gcggcctccg acggtgaccc cgctcgaccg cttctacctc
     2281 gccggcgact ggatcggcac cggcatcccg gcgacggtcg agagcgcgtg cctctccggg
     2341 catgccgccg cccgcgccgc ggcgcggtat tgaaggcatg tcggcgagcc ggacggatct
     2401 cctctgatgg cgccgctcct ggccggcgcc ggcctctgcc tcctggcgtt cgtcgtgtgg
     2461 ctcgtgacga cgcacttccg cgaacggccg tggttcgatc gtcccggttt cgcgcgctgg
     2521 cgcggcttcg atgcgctcgc gtcgcttctg cgctgggccc tcttcgccgc tggcctgctg
     2581 gcgctgtcgg cgcactcagg ggcgggcgcc gccgtcgctg tggggctgtt tcttttcggg
     2641 ttgggctggc ggctcgcggc ccgaagcgct gcgctcaagc gccgcgccat gcgccgcgcc
     2701 ctcgtggagc tgcgttcgaa gcaccccggc gagccggaga ccgagctgct cgtgcggctc
     2761 gtcctggcgg cccacccgcg ctggggggag gagctggtgc gccagatggc gctcgacaac
     2821 ccggacgtcg acgacatggc gcgcgtcgtg gcgcgcatgg agcagggttt tcggggattt
     2881 cgctaagacg ggccaggccg gggagacccg gcggagcgtc gggcctaggt caacagcggg
     2941 acgatctggt cctggacggc ggtcgtcacc tcgacggtgc gctcgtccat gtagacgtcg
     3001 atctcgcttc ggatgccgaa gtcggccagg tagatgccgg gctcgatcga gaagccggtg
     3061 cgcgggatga gcttgcggtc gtcgatcgtc tcgagcgcgt cgatgttgca gccgttgccg
     3121 tgcacctcga agccgatgct gtgcccggtg cggtgcagga agcggtcggc gtagccgcgc
     3181 ttgcggatgt ggtcccgggt cacctcgtcc acctccgagc cgcgcaccgg cttgccgcgc
     3241 cccaccgact ccttcaggaa ggcgacggcg gcgtcgcgcg cccccgcgac gatgtcccac
     3301 acctcgcggt gcttttctgg caccttcgag ccgaggaagg ccatccaggt gatgtcggcc
     3361 cagatggagc cctccttctc ctcgcggcac cacaggtcga ggagcagcag gtcgccgcgg
     3421 cggatcggcg cgcttccggt ggcccgcggc tcgtagtgcg ggttgccggc gtgggcgttc
     3481 accgcgacga tcggaggctc ggcgaagagc ttgcgcgccc ggaactgctc catcatgaac
     3541 tgctggatgg cgtgctcgct cggctcctgc ccgccgccga cgcgcgacgc gacttcctgg
     3601 aaggtcgcct gcagcacctc gcgcagcccc tgcgcggcgc ggcggtgcga ggcgagccct
     3661 tcggcggacc agcgggcctc gcagtactgc acgaggtcgg cgctgctgac gaccgtgggg
     3721 cccgccgccc ggaccatctc gatcgtcccg ccatccaccc gcgagacgta cggaatcgcc
     3781 ccctggggca cgtactccat cgccacccgg tcgatgccct tgaggatctc ccggagccgc
     3841 tcctgcagct cccgatagcc gacgaaaggg atcctcttcc ccggcagctc ggtgaacaga
     3901 tgcgcctcga tcgcgtggtg cagccaggtc ggctccccct gggcggggag gaggccgaac
     3961 cagcgccgcg tcaccagccg ctcggggccg aggcctgcga cgtgccgggc gatcgggttg
     4021 ctgccgtgga agtcgtagag cagccaggcg ggaagcccca gctccttgag gctcgcctgg
     4081 atcgtctcga gattcatggg ggcgaagggt accacacgcc ccgaagagct atgggtcgca
     4141 ggcgtcgccg agaccgtcgt ggtcgctgtc ggcctgggtc ggattggcga cggcggggca
     4201 gttgtcgcag acgtcgccgc gaccatcgcg atcgccgtcg gcctgcgacg ggttgggcac
     4261 gagcgggcag acgtcgtaga cgctggcgat ggtgtcgccg tccacgtcgg ttccggacgg
     4321 cgcgcacaac ggcgtgatgt tgcgcggctg gccgttcgag gaggttccga gcgacccgga
     4381 cccgcaggcg ttgtagccgc ccaccaggta gtaatagatc tgcccgaccg gcggaacatc
     4441 ggggtcggtg aaggtcccct gcggcacctc aggctggagg cactgggagg aggacaggta
     4501 ctcgccgggc atgttcggac cggcgatgcc gcggtagacg ccgtagattt gtgtctgcgc
     4561 cagcagcagc cacgagtact tcccgggcac ggcgccgtcg gcgacgatgg tgtcggagac
     4621 ggttcccggc gacccgctca cccaatgggt gatcggcgcg caatcggccg cgtcgtcgac
     4681 gccgtcgttg tcgtcgtcgg gatcggtgca gttgaacaaa ccgtccaggt cggtgtcggc
     4741 gtcgttgtcg atgaggctgt cgcagttgtc gtcgaggccg ttgcaggtct ccggggacgg
     4801 ggtccccggg acgcagattc ccggcgtgcc attgatgcag ccgggggcgg acgaaaggca
     4861 ctgacccgcg ccgcaggtga cgtcggggat attgtcgtcg g
//
LOCUS       DASRIQ010000253         5413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_631370, whole genome shotgun sequence.
ACCESSION   DASRIQ010000253 DASRIQ010000000
VERSION     DASRIQ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631370"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFD06_06300"
     CDS             <1..476
                     /locus_tag="VFD06_06300"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN03182.1"
                     /translation="FAPGTRRQAGAETEMAQVSRHMTCRQLGYHMRRVLPGNVWPAFH
                     YETGEEEMFLVWKGTMKVRTPQGIFRVGPGDLVAMPTGPAHAHQFVNDGTEPCEIFCL
                     GVDRPQSLAFYPDTNRIMVSERGEFYRVADRREDYWEDDPTVARAPVGPDEPPDS"
     gene            501..794
                     /locus_tag="VFD06_06305"
     CDS             501..794
                     /locus_tag="VFD06_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN03183.1"
                     /translation="MFIVELTYKAPLARIDAQMRAHVAFLRRYYAAGNFLMSGRQIPR
                     TGGIIVAMADSRERIEAIMAEDPFCRHGLAEARIIEFRASQHADDVPSRVTQP"
     gene            903..4751
                     /locus_tag="VFD06_06310"
     CDS             903..4751
                     /locus_tag="VFD06_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HZN03184.1"
                     /translation="MPRGHVYLATSLFLIALLCPASLARAHNYGDVSVAFEGVLEVTQ
                     SVQTAPQLGTEAVSLTAHKPTVIRAYLSARRYVHVNPLLQYNLHNPSFNPISVDGTLT
                     IKQGSTVIATLPASNGPLAVVPWHSPNPEVKNSSLNFEWASPPDGAVTFTLDLTSGTS
                     GIAALDPPSQQRTFTHNERLRIQAVKLELDHDNNPSTPPTKTPNATMVGNGKDWFLKS
                     GPLAPCKLQYWVNPTPLPWTTDIAFATSTDLLNELAAMKSQGTVTYDNVYGWWQDGVA
                     SNGLSYQPYCAYNHTVGAYGNTDPTRYQRTFTHELYHTYHQNHLADNQGNPTCDAHPG
                     TDPGPEHPNGEIGVVGWDTQADLPVPNEKLDVMVAGKLTAEAWLTPERYQTMWNRWMG
                     TTPEQVLDPCHTNWWQFIPPDLFRPLGPMQPFEEFGRSTDFIHIGGAVDLDGTGFLDP
                     AWRLSRVVATDPPGGEGAFGIIIVNSAGNPLGEWRFDPEFHVDSEEAGPASFQFKVPT
                     PLDAGFNIDLREIRLVGPTGMVLDVLRLSPSPPVVEMTAPSPLRPDLTEPTTLQWEAS
                     DPDLDPLTCSVQYSPDNGQSYLALAGALTGATMSIDPARLPGGSTAFFRVLCTDGIRT
                     AADTAGPFAVPRKVPQVEIADPIPDPIGPVTVCAGRPMTMIGFGSSPDDGVLPQEQLE
                     WSSDLQGSLGTGNTLGVTLEEGTHVVRLVGTDRAGDTGSASISIAALSTLIDTDGDGV
                     PDCSDRCPTVPGPWSNDGCPEEEIVDLGVLVLPGVGPDPPPEPRLVFESPSEVHFNVT
                     VGNQRAVPLMVQALRYHLTEPAGAVRVSDVSLGGISIPPGGSFVKTDAFVDVFADPGV
                     SPPDGTWTVVVEAVLGINETSGIAEQIFVVDRTLSFPLASVVPSNPMPAQGSQAAACV
                     FIDMRNTPERLGSYGAALRWNPTVLRFLGFTGGDAPFDTPAVDTANVGTGEVRFSDMA
                     PQGAGGDLFVACFDFEVLGPAGQATQLDLRLTSLFTATSFTDILAMAAVNDAASSVQL
                     ACTIGDANLDGDINSGDALVVLAHEIGRTIPPEPKLAIDAGCGDANGDGATNSIDANI
                     ILSFEVGLPILPSFPLGTSNVTIHRCPVCTGATAAAPAESLAPRAAAPPGGGTIVTDI
                     VPPRGRVRPAEEFEIAVVLDLSAVDHRLGSYGAELTWDPKALRFAGVSSAEFSTPLVN
                     QGEAERGRLRFAAASPSGAEGRVEAVRLRFVALRALPKPERAFDLRFSSLGATGPGFE
                     NLLPLVGGEHGGRLGAEEGE"
     gene            4748..>5413
                     /locus_tag="VFD06_06315"
     CDS             4748..>5413
                     /locus_tag="VFD06_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03185.1"
                     /translation="MSTRRGLLMTVLVAGALAGAADAAPMAEVRPSPGVAASGQMLSV
                     AIDIDMATTGHPLGSYGAALRWNPAYLHYMSDSGGGVAPFDGATVNRSDVGAGILRFA
                     DASAAGAGGRINVQNITFLVIAEPCLTAQLDLSVTSLFSADTLQDLLPVFAVHDGAVN
                     VNDFFLNLQVIGASDTFLSWNAIAGASSYDVIRGEVASIFDDGASIRLGSVVCLENGS
                     FDTT"
BASE COUNT          863 a         1944 c         1775 g          831 t
ORIGIN      
        1 agttcgctcc cggcacccgg cgccaggccg gcgcggagac cgagatggca caggtctcgc
       61 gccacatgac ctgccgccag ctcggctacc acatgcggcg ggtcctgccc ggcaacgtct
      121 ggccggcctt ccactacgag acgggggagg aggagatgtt cctggtctgg aaggggacga
      181 tgaaggtccg caccccccag gggatcttcc gcgtggggcc gggggacctg gtcgcgatgc
      241 cgacgggccc ggcgcacgcg caccagttcg tcaacgacgg gaccgagccg tgcgagatct
      301 tctgcctcgg cgtggaccgg ccgcagagcc tcgccttcta ccccgacacg aacaggatca
      361 tggtgagcga gcgcggagag ttctatcgcg tcgccgaccg ccgcgaggac tactgggaag
      421 acgaccccac ggtggctcgc gctccggtcg gacccgacga gccgcccgac tcgtaggatc
      481 gggcgtcgga gaggagaccg atgttcatcg tcgagctcac ctacaaggca cctctcgcgc
      541 gcatcgacgc gcagatgcgc gcccacgtcg cgtttctcag gaggtactac gcggcgggga
      601 acttcctgat gtccggccgg cagatcccgc gtacgggcgg catcatcgtg gccatggccg
      661 actcgcgcga gcggatcgag gccatcatgg ccgaggatcc gttctgccgc cacgggctcg
      721 cggaggcgcg catcatcgag tttcgtgcca gtcagcacgc cgatgatgtc cccagccgag
      781 tcacgcaacc ctgagcttgc cgcctttttt gcccgagacg tgaggaagtc gcggggctcc
      841 ggcgttcttc cagtgtgaac gccagagcct ccggggctcc gcactcagtg gagggcaaga
      901 acatgccgcg cggccacgtg tacctcgcaa cctcgctgtt cctgatcgca ctcctgtgcc
      961 cggcttcgct cgcccgcgcg cacaactacg gagacgtctc ggtcgccttc gagggcgtgc
     1021 tggaggtcac gcaatcggtg cagaccgcgc cgcagctcgg gacggaggcc gtctcgctca
     1081 cggcgcacaa gccaacggtg atccgcgcct acctctcggc gcggcgctac gtgcacgtca
     1141 acccgctcct ccagtacaac ctccacaacc cgtccttcaa cccgatctcg gtggacggca
     1201 ccctcacgat caagcagggt tccactgtca tcgcgacgct gccggcgagc aacggacctc
     1261 tcgccgtcgt cccctggcac tccccgaacc cggaggtgaa gaacagcagc ctcaacttcg
     1321 agtgggcgtc cccgccggac ggcgcggtga cgttcacgct cgacctcacg agcggcacat
     1381 ccgggatcgc ggccctcgac cccccgtcgc agcagcggac gttcacgcac aacgagcggc
     1441 tgcggatcca ggcggtcaag ctggagctcg atcacgacaa taacccgtcc acgcccccca
     1501 ccaagacacc caatgcgacg atggtcggga atgggaagga ctggttcctg aagtccggtc
     1561 cgctcgcccc ctgcaagctc cagtactggg tcaacccgac cccgttgccg tggacgaccg
     1621 acatcgcgtt cgccacttca acggaccttc tcaacgagct ggccgcgatg aagagccagg
     1681 gcaccgtcac gtacgacaac gtgtacggct ggtggcagga cggagtcgcc agcaacggcc
     1741 tgtcgtatca gccgtactgc gcctataacc atacggtggg ggcctacggc aacaccgatc
     1801 ccacgcgcta ccagcgcacc ttcacccacg agctgtacca cacctatcac cagaaccacc
     1861 tggcggacaa ccaagggaat ccgacctgtg acgcgcatcc cgggaccgat cccggccccg
     1921 agcacccgaa cggggagatc ggggtcgtcg gctgggacac gcaggccgac ctgccggtgc
     1981 cgaacgagaa gctcgatgtc atggtcgccg gcaagctcac ggcggaagcc tggctgacac
     2041 ccgagcgcta tcagacgatg tggaacaggt ggatggggac gactccggag caggtcctcg
     2101 acccctgtca caccaactgg tggcagttca taccgccgga cctcttccgg ccactgggac
     2161 cgatgcaacc cttcgaagaa tttggacgaa gcaccgactt cattcacatc ggcggcgccg
     2221 tcgatctcga cggcacgggc ttcctggatc cagcctggag gctcagccgg gtcgtcgcga
     2281 cggatcctcc cggcggggag ggagccttcg gcatcatcat cgtcaacagc gccggcaatc
     2341 ctcttggcga atggcgcttc gaccccgagt tccacgtcga ctccgaggag gcgggaccgg
     2401 ccagcttcca gttcaaggtc ccgacgccgc tcgatgccgg cttcaacatc gatctccgtg
     2461 agatccgtct cgtcggcccg accggaatgg tcctcgacgt cctgcgcctc agcccgagcc
     2521 ctcccgtcgt ggagatgacc gctccgtccc ccctgagacc ggatctgaca gagccgacga
     2581 cgctccagtg ggaggcttcc gacccggatc tcgatcccct cacgtgctcg gtccagtaca
     2641 gccccgacaa cgggcagagc tacctcgctc tcgccggtgc gctcaccggt gcgacgatga
     2701 gcatcgatcc tgctcgcctg ccgggagggt cgacggcttt cttccgggtc ctgtgcacgg
     2761 acggcatccg cactgctgct gacacggccg gtccgttcgc ggtgccccgc aaggtgccgc
     2821 aggtggagat cgccgatccc atcccggatc ccatcggccc ggtgacggtc tgcgccggga
     2881 ggccgatgac catgatcggt ttcgggtcca gcccggacga cggcgtcctg cctcaggagc
     2941 agctcgagtg gtcctccgac ctccaagggt ccctcggcac cgggaacaca ctcggggtca
     3001 cgctggagga ggggacccat gtcgtgcgcc tggtcggcac cgaccgcgcc ggcgacacag
     3061 gaagcgccag catctcgatc gcggcgctct ccaccctcat cgacaccgac ggtgacgggg
     3121 tccccgactg ctccgaccgc tgcccgacag tgcccggccc ctggtcgaac gacgggtgcc
     3181 cggaggagga gatcgtggat ctgggggttc tcgtcctccc gggcgtggga ccggatccgc
     3241 cgccggagcc caggctcgtg ttcgaaagcc cctccgaggt tcacttcaac gtgacggtgg
     3301 ggaaccagcg cgcggtgccg ctgatggtgc aggcgctgcg ctaccacctg accgagccgg
     3361 ccggagccgt gcgcgtgagc gacgtgtcgc tcggagggat ctccatccca cccggaggct
     3421 cgttcgtgaa gaccgatgcg ttcgtggatg tcttcgccga ccccggggtg agtccgcccg
     3481 acggcacgtg gacggtggtg gtggaggccg tcctggggat caacgagacc tcggggatcg
     3541 ccgagcagat cttcgtcgtg gatcggacgc tgtcgttccc gctcgcgagc gtcgtgccct
     3601 cgaacccgat gccggcgcag gggagccagg cggcggcctg cgtcttcatc gacatgcgca
     3661 ataccccgga gcggctcgga agctacggcg ccgccctgag gtggaacccc acggtgttga
     3721 gattcctggg cttcacgggt ggagacgcgc cgttcgacac gccggccgtc gacacggcca
     3781 acgtcgggac cggcgaggtc cggttctccg atatggcccc ccagggcgcc gggggcgacc
     3841 tcttcgtcgc ctgtttcgac ttcgaagtcc tgggcccggc cgggcaggcc acgcagctcg
     3901 acctccggct cacgtcgttg ttcaccgcga cgagcttcac cgacatcctc gccatggcgg
     3961 cggtcaacga cgccgcgtcg agcgtccagc tggcgtgcac gatcggcgac gcgaacctgg
     4021 acggggacat caactcgggc gacgcgctcg tcgtcctggc gcacgagatc ggcaggacca
     4081 tccctcccga gccgaagctg gccatcgacg ccggttgcgg cgacgccaac ggcgacgggg
     4141 ccaccaactc gatcgacgcc aacatcatcc tgtcgttcga ggtcggcctg ccgatcctgc
     4201 cgtccttccc gctcgggacg tcgaacgtca cgatccaccg ctgtcccgtg tgcacgggcg
     4261 ccacggccgc cgcgccggcc gagtcgctcg cgccccgcgc cgccgcgccg ccgggcgggg
     4321 ggaccatcgt cacggacatc gtcccccccc gtggccgggt gcgccccgcg gaggagttcg
     4381 agatcgccgt cgtgctcgac ctgagcgccg tggaccaccg gctcggaagc tacggcgccg
     4441 agctgacgtg ggacccgaaa gcgctccgct tcgccggcgt gtcgtccgcg gagttctcca
     4501 cgccgctcgt gaaccagggc gaagcggagc gcgggcgtct gcgcttcgcc gccgccagcc
     4561 cttcgggcgc ggagggtcgc gtcgaggcgg tgcgactgag attcgtcgcg ctgcgggcac
     4621 tgcccaagcc ggagcgcgcc ttcgatctgc gcttcagctc gctgggcgcc acggggcccg
     4681 gcttcgagaa cctcctcccg ctcgtgggtg gagagcatgg cggcaggctc ggcgccgagg
     4741 agggagaatg agcacgcggc gcggtctctt gatgacggtc ctggtggccg gggcgctggc
     4801 cggcgctgcg gacgcggctc cgatggctga ggtgcgtccg tctcccggcg tcgccgccag
     4861 cgggcagatg ctctcggtcg ccatcgacat cgacatggcg acgacaggcc acccgctcgg
     4921 gagctacggc gcggccctgc gctggaaccc ggcgtatctg cactacatgt cggacagcgg
     4981 cggcggagtc gcgcctttcg acggcgccac cgtgaaccgc agcgatgtcg gcgccggtat
     5041 cctgcgcttc gccgacgcct cggcggccgg cgccggcggg cgcatcaacg tccagaacat
     5101 cacgttcctc gtgatcgccg aaccgtgcct gacggcccag ctcgacctga gcgtcacgtc
     5161 cctcttctcc gccgacaccc tccaggacct gctgccggtg ttcgcggtcc acgacggagc
     5221 cgtcaacgtg aacgacttct tcctcaacct ccaggtcatc ggggcctccg acacgttcct
     5281 cagctggaac gcgatcgcgg gcgcctcgag ctacgacgtg atccggggag aggtcgcatc
     5341 catcttcgac gacggcgcct ccatccgtct cggaagcgtc gtctgcctgg agaacggctc
     5401 gttcgacacg acg
//
LOCUS       DASRIQ010000254         3928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1925771, whole genome shotgun sequence.
ACCESSION   DASRIQ010000254 DASRIQ010000000
VERSION     DASRIQ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925771"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFD06_06320"
     CDS             complement(<1..686)
                     /locus_tag="VFD06_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03186.1"
                     /translation="MRTPGPAPGSDRLLLLGASVRALAASVLRSPRARARYSGGLLLL
                     DYFADDDLAREPRVVFACGMDQRARPRSTAGLGRAALALAADGWRWEGLAFTGGLENR
                     PGLLRLLARRAPILGSDPASIAAVRDPVRLAAFLEKEDLQHAGVSRLDTVPSEGRWLF
                     KRRRGAGGSGVREARSGERRRSGDDLQARLDGCAGSIAFVANGSEARLLGATAEIGSA
                     AVVAALGAPP"
     gene            complement(709..1665)
                     /locus_tag="VFD06_06325"
     CDS             complement(709..1665)
                     /locus_tag="VFD06_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylene-tetrahydromethanopterin dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HZN03187.1"
                     /translation="MESGGAGASGSAGPGVLSVPPKRLLFILAADRHVSPFDVALAHD
                     AGFDAVHLYPEVAPEETRPLTQDLMFARGPKGAAASALFVTTADLARAEAAFRAAREA
                     LLDPFRIHLMIDPKGGYTTAAALLAKVEALSRRRGPGDLRGRTVAILGGTGGVGRAAA
                     GMAAAAGARVLLTSRDTARAQAAAREVADLFGAAVESRAASGEKESAALVAEADVVLA
                     TGAAGARLLSRASLAGMKGPKVVADVNAVPPSGIEGLEPADQERELAPGVFGIGALAI
                     GALKSKVESSLLRDLATSDKALVIDAAAAGRRAAEIVAASQA"
     gene            complement(1696..2190)
                     /gene="fae"
                     /locus_tag="VFD06_06330"
     CDS             complement(1696..2190)
                     /gene="fae"
                     /locus_tag="VFD06_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HZN03188.1"
                     /translation="MKDILIGESLVGEGNEVAHIDLLIGPKNGPVGAAFANALVNQNA
                     GHTALLAVLEPNLIPKPATIMMNKVTMKGAGQAVQMFGPAQMAVAKAVADSVAEGIIP
                     QAEAENLVCIVGVFIHWEAKDNKKIFDYNYKATKESIARAMRGEPSVATVTERKDKAK
                     HPFA"
     gene            complement(2965..3693)
                     /locus_tag="VFD06_06335"
     CDS             complement(2965..3693)
                     /locus_tag="VFD06_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate/uridylate kinase"
                     /protein_id="HZN03189.1"
                     /translation="MSRRRGAVGSVLDRCAFDIVVKVGGSLGRGPAPLRAIVRRLTAL
                     ARRRRVLVVPGGGAFADLVRRERRRLGLDAEAAHRMALRAIDQYGLLLASLHPRASAA
                     ADLAAARRLAVSRRLAILLPSALVDRAPSLERTFRLTSDAIAAWVAGRTGARRLLLLK
                     SVRGIDLSFSDREAAAGLARRGIVDPLFPRLLPTGIAVQVVDGRGAWTLSRTIDAAPP
                     GRSGGARPRANRSVPRRRGRPGRR"
     gene            complement(3690..>3928)
                     /locus_tag="VFD06_06340"
     CDS             complement(3690..>3928)
                     /locus_tag="VFD06_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03190.1"
                     /translation="RFLMDRQVDRIAAAVRAVAPAAGCPVVVCGAGRFLAAEAARRAG
                     MPSLPIEDLLPGYGPGWGTAAPAAALALLAAAVA"
BASE COUNT          496 a         1512 c         1439 g          481 t
ORIGIN      
        1 aacggcggcg cgcccagcgc cgcgaccacg gcggccgatc cgatctccgc ggtcgctccg
       61 aggagccgcg cctcgctccc gttcgcgacg aaggcgatcg acccggcgca cccgtcgagg
      121 cgggcctgca ggtcgtcgcc cgaccgccgc cgctcccccg accgggcctc ccgcacaccg
      181 ctccccccgg cgccccgccg gcgcttgaag agccagcgcc cctctgaagg gacggtgtcg
      241 aggcgcgaca cgccggcgtg ctgcagatct tccttctcga ggaacgcagc gaggcgcacc
      301 ggatcgcgga ccgcggcgat ggaggccgga tcgctcccga gaatcggggc gcgtcgcgcc
      361 aggagacgca ggagcccggg gcggttctcg agcccccccg tgaacgcgag cccctcccac
      421 cgccacccgt cggccgcgag ggcgagcgcc gcgcggccga gaccggcggt cgatcgcggc
      481 cgtgcccgtt ggtccatccc gcacgcgaac acgacgcgcg gctcgcgcgc cagatcgtcg
      541 tcggcgaagt agtccagcag gagcagcccc cccgaatagc gggcgcgcgc ccgcgggctc
      601 cggagcaccg aggcggcgag cgccctcacg ctcgcgccga gaagcagcag gcgatcggat
      661 ccgggtgccg gtccgggtgt ccgcatgcgc gggccgctgc gccggccgtc aggcctggga
      721 ggccgcgacg atctcggcgg cgcggcgccc ggccgccgcc gcgtcgatga cgagcgcctt
      781 gtcggaggtg gcgaggtcgc gcaggagcga cgattcgact ttcgatttca gggcgccgat
      841 ggcgagcgcg ccgattccga agacgcccgg cgccagctcg cgctcctggt ccgcgggctc
      901 cagcccctcg atcccggagg gaggcacggc gttgacgtcg gcgaccacct tgggaccctt
      961 catccccgcg agcgatgcgc gcgacagcag ccgcgcgccg gccgcgccgg tggcgagcac
     1021 gacgtccgcc tccgcgacga gcgccgcgga ctccttctca ccggatgcgg cgcgcgactc
     1081 gaccgccgcg ccgaacagat cggcgacctc gcgcgccgcc gcctgcgcgc gcgccgtgtc
     1141 gcgcgacgtc agcagcacgc gggctccggc cgccgccgcc atgccggcgg ccgcccggcc
     1201 gacgccgccg gtcccgccga ggatcgccac ggtgcggccg cgcagatcgc cggggccgcg
     1261 ccggcgcgac agcgcctcca ccttggcgag gagcgccgcg gcggtggtgt atcccccctt
     1321 cgggtcgatc atcagatgga tgcggaacgg atcgagcagc gcctcgcgcg cggcgcggaa
     1381 cgccgcttcg gcgcgggcca ggtcggcggt cgtgacgaac agcgccgagg cggccgcccc
     1441 tttcgggccg cgcgcgaaca tcaaatcctg ggtgagcggg cgcgtctcct ccggcgcgac
     1501 ctccgggtag aggtgcacgg cgtcgaaccc ggcgtcgtgc gccagggcga cgtcgaacgg
     1561 gctgacgtgc ctgtcggcgg cgaggatgaa gaggaggcgc ttgggcggca cggacaggac
     1621 ccccgggccg gcggagccgg acgcaccggc tccgccggac tccatgaacg ccgcgcttcg
     1681 agagcgcggc gacgatcagg cgaagggatg cttggccttg tccttgcgct ccgtcaccgt
     1741 cgcgacgctc ggctcgccgc gcatggcacg ggcgatcgac tccttcgtcg ccttgtagtt
     1801 gtagtcgaag atcttcttgt tatccttcgc ttcccagtgg atgaagacgc cgacgatgca
     1861 gaccaggttc tcggcctcgg cctgcgggat gatcccctcg gccaccgagt cggccaccgc
     1921 cttggcgacc gccatctgcg ccggaccgaa catctgcacc gcctgccccg cccccttcat
     1981 cgtcaccttg ttcatcatga tcgtcgccgg cttcggtatc aggttcggct cgagcaccgc
     2041 cagaagcgcc gtgtgtccgg cgttctggtt cacgagcgcg ttcgcgaacg ccgcgccgac
     2101 cggcccgttc ttcgggccga tgagcaggtc gatgtgggcc acttcgttcc cctcgccgac
     2161 gagggattcg ccgatcagga tgtccttcat ggcttgctct cctttggtcg aaccgagtgc
     2221 gttggatgaa acgccgatcg agatgcgcgg gatccggcga aggatctagc acgcccgcgc
     2281 gcgcccggtc aatcgcgacg ccgctgggga cggcaccttc ccgtcttgag cgggccggcc
     2341 gcgcgttccc gccggcgctc ctcgcgagcg tcgcctcggc cggagaggcg gcgacagcgc
     2401 tcgagggcgg ggcggagatc gtggatctca aggacccccg gcgcggtccg ctcggcccct
     2461 gctcggcgag gacgctcgcg gccgcgcgcc ggaccgggct ccgcctcgac ccggcgcggc
     2521 cgatgagcgc cgcgctcggt ccggcccgtt gcccgcggac gccccggcgg gccgcttcgg
     2581 ccgcgaggct cggttatgcc tacgtgaagt gcggcctgga gggactcgcg gacctgcccg
     2641 ccgccgtccg ggcgctccgg gccgtggcgc gtgcggcgcg cgccgcctcg ccgcaggtgc
     2701 aggtcatcgc cgccggcttc gccgacgcgg agcggatccg ctccctcccg tggcgtctcc
     2761 tgccggaggc ggcggcgcgc gccggcgtcg acgggtgcct cctcgacacg gcggggaagg
     2821 atggacgcag tctctccgtc tgggtctccc cgcgcgatct cgccgcgttc gtcgccgact
     2881 gccgtcggcg cggcctgctg tgcgccctcg ccggctcgat cgcggcaaac gatctgccgg
     2941 cgatcgtcgc gctcggcgct gacatcatcg gcgcccgggg cgccctctgc gacgggggac
     3001 ggaccggttg gctctcggcc gagcgcctcc ggatcttccg ggcggcgctg catcgatcgt
     3061 tcgagacagg gtccaggccc cccgtccgtc gaccacctgc acggcgatcc cggtcggcaa
     3121 caggcgcggg aagagcgggt cgacgatccc ccgccgcgcc agccccgccg ccgcctcacg
     3181 gtcgctgaac gacaggtcga tcccgcgcac gctcttgagg agcaggagtc gtcgcgctcc
     3241 ggtccgaccc gccacccacg ccgcgatcgc gtcggaggtc aggcggaacg tccgctcgag
     3301 ggacggcgcg cggtcgacga gcgccgacgg caggaggatc gcaagtcgcc ggctcacggc
     3361 gaggcgccgc gctgcggcga gatcggccgc cgccgaagcc cgcggatgca gggacgcgag
     3421 gaggagcccg tactgatcga tcgcgcggag cgccatccgg tgcgcggcct cggcgtcgag
     3481 cccgaggcgc ctccgctccc ggcggacgag gtccgcgaac gcgccccccc ccggcacgac
     3541 gagcacccgc cggcgccgcg ccagggccgt gagccgccgg acgatggcgc gcagcggggc
     3601 cggtccgcgt ccgagactgc cgccgacctt gacgacgatg tcgaacgcgc agcgatcgag
     3661 gaccgacccg actgcgccgc gccgcctgct cacgcgacgg cggcggcgag aagcgccagg
     3721 gccgccgccg gggcggccgt gccccacccc gggccgtatc ccggcaggag atcttcgatc
     3781 gggagggaag gcatcccggc gcgccgggcc gcctccgccg ccaggaagcg gccggcgccg
     3841 cagacgacga ccgggcaccc ggccgccgga gcgaccgccc ggacggcggc tgcgatccga
     3901 tccacctggc ggtccatgag gaagcgcg
//
LOCUS       DASRIQ010000255         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_879724, whole genome shotgun sequence.
ACCESSION   DASRIQ010000255 DASRIQ010000000
VERSION     DASRIQ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879724"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="VFD06_06345"
     CDS             complement(<1..1916)
                     /locus_tag="VFD06_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN03191.1"
                     /translation="MAIYGALGFGIGLTLTFVGYVVDFWALYGVLPARINFAMIQGLH
                     RVTPVHYFTAAFAPILAIVGALAGWLQDKVLFYSRHLADLVTERTEDLRASQERYELA
                     ARGANDGLWDWDLVRNTIYYSPRWKEALGHQAADVANAPGAWLDRVHPDDRPGLEARI
                     RSHLAGASDHLVAEYRIQHADGSQRWMLSRGIAVRDPATGNAYRIAGSQTDIDDQKRM
                     EEQLRRLALHDPLTGIANRTLFLDRLSRAFGRARRRRRTESLAIIFVDIDRFKNINDS
                     LGHPIGDRVLAILAERFRSCLERTFPWAEAEQDEDAVRRGDRLLSWTIARMGGDEFIV
                     LLEEIRSLRDATQVVQALEAEVSRSVEVEGRDLFVTLSTGIVLGPGVYERPEDLLRDA
                     DTAMYRAKAGGRGRFEIFDENMLARAQEQLRLETDLHRALAQNEFQVVYQPIYELEGG
                     RLRGFEALLRWHHPERGLIPPSRFIPLAEEIGLIGRLGRMMLVEACRRLKRWQDLDRE
                     WRDLTLATNLSLRQIYNPGLEEEITGILEETGVDPATLYFEITENTLIEHPKQVIKML
                     TRLKRLGFGVAIDDFGTGYSSLAMLQDLPVDILKIDQVFVSRMDERKGRQIVATIVSL
                     ARALGHEVIAEGIET"
     gene            complement(1960..>2148)
                     /locus_tag="VFD06_06350"
     CDS             complement(1960..>2148)
                     /locus_tag="VFD06_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03192.1"
                     /translation="APGGFGWMGWILPALLGAMAGVGAQIILRHRRRPVARRGDETQP
                     EWLTDEDARDADGRRDAA"
BASE COUNT          313 a          750 c          747 g          338 t
ORIGIN      
        1 gtctcgatcc cctcggcgat cacctcgtgg ccgagggcgc gcgccaggct cacgatggtc
       61 gcgacgatct ggcgcccttt ccgctcgtcc atgcggctga cgaacacctg gtcgatcttc
      121 aggatgtcca ccggaagatc ctgcagcatg gcgagcgacg aatagccggt tccgaagtcg
      181 tcgatggcga cgccgaagcc gagccgcttc aggcgcgtca gcatcttgat cacctgcttc
      241 gggtgctcga tgagggtgtt ctcggtgatt tcgaaataga gcgtcgcggg gtcgaccccc
      301 gtctcctcga ggatgccggt gatctcctcc tcgagcccgg ggttgtagat ctgccggagc
      361 gacaggttcg tcgccagcgt caggtcgcgc cattcacggt cgaggtcctg ccagcgcttc
      421 aggcgcctgc aggcctccac gagcatcatc cgacccagcc ggccgatgag gccgatctct
      481 tcggcgagcg ggatgaaccg cgacggcggg atgaggccgc gctccgggtg gtgccagcgg
      541 aggagcgcct cgaatccccg cagccgccct ccctccagct cgtagatcgg ctggtacacc
      601 acctggaact cgttctgcgc cagcgcccgg tgcaggtcgg tctccagccg cagctgctcc
      661 tgggcccgcg ccagcatgtt ctcgtcgaag atctcgaagc ggccgcggcc gcccgccttc
      721 gcccggtaca tggcggtgtc ggcgtcgcgg aggagatcct ccgggcgctc gtagacgccc
      781 gggccgagga cgatcccggt gctcagcgtc acgaacaggt cgcgcccctc gacctccacc
      841 gagcggctga cctccgcctc gagcgcctgc accacctgcg tcgcgtcgcg cagtgagcgg
      901 atctcctcga gcagcacgat gaactcgtcc ccgcccatcc gggcgatggt ccacgacagc
      961 aggcgatcgc cccgccggac ggcgtcctcg tcctgctccg cctccgccca cgggaaggtg
     1021 cgctccaggc acgagcggaa gcgctcggcg aggatagcca ggacccggtc cccgatgggg
     1081 tggccgaggc tgtcgttgat gttcttgaag cggtcgatgt cgacgaagat gatcgccagg
     1141 ctctcggtac ggcggcggcg tcgggcgcgt ccgaaggcgc gcgacaggcg gtcgaggaac
     1201 agggtccggt tcgcgatgcc ggtgaggggg tcgtggagcg ccaggcgtcg cagctgctcc
     1261 tccatccttt tctgatcgtc gatgtcggtc tgggagccgg cgatgcggta ggcgttcccc
     1321 gtggctgggt cccggaccgc gatgccgcgc gacagcatcc agcgctgcga tccgtcggcg
     1381 tgctggatgc ggtactccgc gacgaggtgg tccgacgcgc cggcgaggtg gctccggatg
     1441 cgcgcctcga ggccggggcg gtcgtcggga tggacccggt cgagccaggc ccccggcgcg
     1501 ttcgcgacgt ccgcggcctg gtggccgaga gcctccttcc agcggggcga gtaatagatg
     1561 gtgttgcgga cgagatccca gtcccacagc ccgtcgttcg cgccgcgcgc cgccagctcg
     1621 tagcgctcct ggctggcccg gaggtcctcg gtccgctcgg tgacgaggtc ggccaggtgc
     1681 cgcgagtaga aaaggacttt atcctgaagc catccggcca gcgcgccgac gatcgccagg
     1741 atcggcgcga aggcggcggt gaagtagtgg accggcgtca cccggtgcag tccctggatc
     1801 atcgcgaagt tgatgcgggc cgggaggacg ccgtagagcg cccagaagtc gacgacgtag
     1861 ccgacgaacg tcagggtgag tccgatgccg aagccgagcg ccccgtagat cgccatcctc
     1921 gacgggtgcg ccgggaccct cctcacccag gacctgcgct caggcggcat cccgccggcc
     1981 atcggcgtcc ctcgcatctt catccgtcaa ccattcgggc tgcgtctcgt ccccgcgccg
     2041 agcgacgggc cgccggcgat ggcgcaggat gatttgcgcc ccgacgccgg ccatcgcccc
     2101 gagcagcgcg gggaggatcc atcccatcca cccgaagccg cccggagc
//
LOCUS       DASRIQ010000256        10550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1413488, whole genome shotgun sequence.
ACCESSION   DASRIQ010000256 DASRIQ010000000
VERSION     DASRIQ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413488"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1857)
                     /gene="lepA"
                     /locus_tag="VFD06_06355"
     CDS             complement(61..1857)
                     /gene="lepA"
                     /locus_tag="VFD06_06355"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZN03193.1"
                     /translation="MDASLIRNFCIIAHIDHGKTTLSDRILELCGALSMREMDEQVLD
                     NMDLERERGITIKAHAVALEYKSGDGKTYRLNLIDTPGHVDFAYEVSRSLSACEGALL
                     VVDATQGVEAQTLANSYMAVDHHLHIIPVINKIDLASAEPERMKEQIENIIGISAENA
                     LPCSAKAGTGVQEILEAVVREVPFPKGDPDAPLKALVFDSWFDAYRGVTILARVIDGT
                     FRKGTKIMLMATGRTYDAEEIGLMTPKAVPAESLSAGEVGYVVANIRNATEVKIGDTI
                     TEARRPTATAFPGFQEMKPMVFAGLFPIGESGYDTLRDALEKLRLNDASFSVEPENSE
                     ALGFGFRCGFLGLLHMEIVQQRLERHFGLSLITTAPSVRYRITTTDGEVLEVSNPARL
                     PEPQRIDQWEEPVITCLILTPPEFLGGILALCQDKRGVQKDLRYVTTTRVLVSYDLPF
                     NEVVIDFYDKLKALSRGYASLDYHHTGWWKSDMAKLDILVNGAPVDALSLIVHRERAA
                     QKGRALAQKLRQEIPRQLFEVAIQAAIGGKIVARENVAALRKNVLAKCYGGDITRKRK
                     LLEKQKEGKKRMKRVGRVDLPQEAFLAILKVD"
     gene            complement(1879..2928)
                     /gene="tsaD"
                     /locus_tag="VFD06_06360"
     CDS             complement(1879..2928)
                     /gene="tsaD"
                     /locus_tag="VFD06_06360"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZN03194.1"
                     /translation="MTAPPSSGPLLLGIESSCDETAAAVLRGSTILSNVIASQTELHA
                     PNGGVVPELASRHHLEQIDLVIDTALERAGCGLADLDGVAVTYGPGLVGCLLVGLQAA
                     KAIAWGRGLPLLGVNHLEGHLRSVWLEHGEIPSPSMNLVASGGHTGLYLIEGDEPPRR
                     IARTRDDAAGEAFDKVAKMLGLPYPGGPVVDRLAPSGDPKAVRLSPPKMSDGSLDFSF
                     SGFKTAALRHAQARGLTRAYPAPDQDAPSQETRDLLASFQASVVAFLVDRLMTAADEA
                     GARAVCLSGGVACNSELRRRVAEEAKRHGLAMYSVRRDLAVDNAAMIAEVGRRRLLEG
                     RAASLDLNAEPGLEL"
     gene            complement(2930..3994)
                     /gene="mtnA"
                     /locus_tag="VFD06_06365"
     CDS             complement(2930..3994)
                     /gene="mtnA"
                     /locus_tag="VFD06_06365"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZN03195.1"
                     /translation="MFKTIEWGEGGVLLLDQRRLPEEEIYLTLRTPEEVAEAIRGMAI
                     RGAPAIGVAAAFGIALGFQPGVRVPGTEDPSARFEALAALLGGTRPTAVNLFWAIKRM
                     RRRFEAHRHEGAGPLAAALLSEARAVHAEDIAANRSMGRHGAALLPGEGAVLTHCNAG
                     ALATAGYGTALGVVRAAVEAGKRIAVFADETRPFLQGARLTAWELQKDGIPVTVLCDG
                     MAGHLFQKKAIGACIVGADRIAANGDVANKIGTYQVAVLARAHEVPFYVAAPTSTVDL
                     SLATGASIPIEERSADEVVEIAGKRVVPRGVPVLHPAFDVTPHALVTAIVTENGVARA
                     PYEASLASLCGGAGRAGGAS"
     gene            4302..>10550
                     /locus_tag="VFD06_06370"
     CDS             4302..>10550
                     /locus_tag="VFD06_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN03196.1"
                     /translation="MERSRSGPSFTSRIRGIALRLLATGGLAALVLATLTPIRAGRGA
                     AEAPGYDRTQRDSSEAFFRRNRLPHGHPRIEGLSPFGTQIRGFAGPAEGSSRVSTAAG
                     FFDLTRPEEIASLPAPLRLRSEVPLPGGRGGLVAGIDLVQVRAAVLQGRGESGMRLLL
                     EPYGRVLGLVPERGFIVEVRDRAAAEALAREPFVDAASPYHPAFKIDPNLGRTPFIEA
                     KRAASPILRLQVIAWQALDASGRDELRAGLSRLLGGGAVRIDGDDRLLYVEAEASKVA
                     AIAALPGVEAVAEEPELMLSNAEAPSVVMVGSVEDTLGARPYHDLGVDGGGIDTNGDG
                     ARVNDGTDSVPPQIVAVTDNGISIDTPSFSQTATQTTTITSPVGAKHRKVHAIQSVAD
                     AGDNCDGVLSGSGSHGNIVASAIAAWPSALGVYATKTTLPRNPIVSGIALDGVARGAR
                     IIMQDAANFTRCTIDELIEQGGNISAGNVTTRLTQGICPKSGGTGPICTGVSGGGSET
                     HLHVMPFGVPNFDNILDNPQNGAYSVEASQIDTFLVNNRDFMVFTPIGNHGANPKNLT
                     QRRIPDLFDGTALDNDPNFPSANQINPPATAKNVVSVGSHRTDMQTFAGTFNEEEVSS
                     PWASRGPATNASLRMAPIVTSIGEDFSGVFGAPGIGGVAAFRSRDNDSTAPIDAQLDE
                     LNFGTSYAAAYAGGAGAIVRDYFAQGFYPGGTRRTADRMSNVSGALVKAALVASANFL
                     EENGRSQYPTLTDRFVAQARAGNVGMVSGVNVGVLGNMEQGYGRVQLSNVLPIPNWPT
                     VIGIGKPDTIEHSAAALLVWDDIGTGEPPINNTTTVIEHTFLVDAPNTLVLGGGGRAV
                     SLGALRIALAWIDPPGTALGDGALINDLDLEVESPGPDGSLATTADNLIYDGNLYNLG
                     LGPRAGQWSAPRVAADLADLRNPVEAVHIPSDLNGDGNPADSRLYAGTWRVRVKRGVG
                     GAVPGSITRIDGPSEDANGNFRLETLPLPSEDLDADGLLDANGQPFALVVSGPVMGTG
                     TQTWGGAPHSLPQSATHLDQPTYGCADDLVVNVFDPDGTIAGIGAATTLTVQDALGNV
                     LDSEQGFTFTEAPAGSHGFRSAKIPVRVIAPTPVSNNGILETDTGQFIVADYADSPVP
                     GQARATVACNPSLFAGVLQIRDEADGAALFTGGCDRDQYPDAGESLTYTVALLNADRG
                     DDYTDVTATLVPSGLGAAAIQVLDSPKAIGRLPGGQTAGISFSLRVNATTLNALPVAS
                     RIATLTLTLDSTSRHKVIGRQSFAFTHALNSDKETFHYSTDYPAGGREIRDLNRNLQI
                     DKADQIDPFTGIEVPDEDLVFSTLWLSDGGVVRNILGEDLDNDGVRDPNEADIIPNNG
                     LLDKGILALPGGPSANDKVPFSFDANNGGFYPVRHPNSEAGAGGTQVVWEFHADGLCG
                     FQTANPDGDGSPRFQNNGAGIWHTGDGDTFTPDEFSTGCDNYFMPQNLATPAQAEFIF
                     DMLESPIVAKVHQTTDARGFPYTVEFQRLAMNLNHQTIDAYAGGQVNLDSDVDSDDRN
                     CLLCQVFYPRFGGAYYGVARFNTYENGVDQNGAGEVRQRTFGPKVDPDNSINAFGYVS
                     GDETGFSGFTSDSNPVSSSPIPTAPPDLLPYPIPGGPLPLASDGTPALNNVRGPTRNF
                     ELSLVNYQDSYVYFPTGPGAYEPGGYFNPGPTGNRWMFGIGFFVIESTTLLNDYGLAV
                     DDAVLEWDEFHPLDESQFVPAHTPACQRFGQPGQPAGQQCATIVLDRTSLYECDESVS
                     VTVHDAKKAGQGSVVVQAASDSDGTPFFAANGTGFLPAKSFTLPEVTPGVFRGNITVT
                     SQFNNAATVFVTPASDQILSVYYNDPLCDADGDTQVAESAFDNIDGDGIASASDKCPA
                     VYDPAQPDADGDGRGDLCDNCPALANATQVDSDADGIGDACDFDDVDVDGVANEVDNC
                     PDVYNPLQTPGAAGRGTACDQTTDRDGDGVTDRNDNCVRTSNANQLNSDTDFLGNVCD
                     GDCASPAATNATGGVCQRTNTTICTTNANCPTTGTCNITSSTVCTNNGQCPQGEQCIG
                     IVQETCMRA"
BASE COUNT         1652 a         3831 c         3576 g         1491 t
ORIGIN      
        1 ggtcgaggcg ctcgccggcc atcgacttga ccgggtagcg ccacagctcc gcgacgtgca
       61 tcagtccacc ttcaggatcg cgaggaacgc ctcctggggc agatcgaccc ggccgacgcg
      121 cttcatgcgc ttcttcccct ccttctgctt ctcgaggagc ttgcgcttgc gggtgatgtc
      181 gccgccgtag cacttcgcca ggacgttctt gcggagcgcg gcgacgttct ctcgcgcgac
      241 gatcttgccg ccgatggccg cctggatcgc cacctcgaac agctgccggg ggatctcctg
      301 gcgcagcttc tgcgccagcg cgcggccctt ctgcgccgcc cgctcgcggt gcacgatgag
      361 cgacagggcg tccaccggcg cgccgttcac caggatgtcg agcttcgcca tgtcggattt
      421 ccaccagccg gtgtggtggt agtcgagcga agcgtagccg cgcgacaggg ctttgagctt
      481 gtcgtagaag tcgatcacca cctcgttgaa cggaaggtcg tacgacacca gcacccgggt
      541 ggtcgtgacg taccggaggt ccttctgcac cccgcgcttg tcctggcaga gggccaggat
      601 gccgccgagg aactccggag gggtcaggat gaggcaggtg atgaccggct cctcccactg
      661 atcgattctc tgcggctccg gcagacgggc cgggttcgac acctcgagca cctcgccgtc
      721 ggtcgtggtg atgcggtagc ggaccgacgg ggcggtcgtg atgagcgaca ggccgaagtg
      781 gcgctcgagg cgctgctgca cgatctccat gtgcaggagt cccaggaagc cgcagcggaa
      841 gccgaagccg agcgcctcgc tgttctccgg ctcgaccgag aacgacgcgt cgttgagccg
      901 cagcttctcg agggcgtcgc gcagcgtgtc gtagccgctc tcgccgatcg ggaagagccc
      961 ggcgaacacc atcggcttca tctcctggaa gccggggaac gcggtcgcgg tcggccgccg
     1021 cgcctcggtg atggtgtcgc cgatcttcac ctcggtggcg ttgcggatgt tggcgacgac
     1081 gtagccgacc tcccccgccg acagcgactc cgccggcacc gccttgggcg tcatcaggcc
     1141 gatctcctcg gcgtcgtagg tccgcccggt cgccatcagc atgatcttcg tccccttccg
     1201 gaaggtgccg tcgatgacac gcgccaggat ggtgacgccg cggtaggcgt cgaaccagga
     1261 gtcgaagacg agcgccttga gcggcgcgtc ggggtccccc ttcggaaacg ggacctcgcg
     1321 caccaccgcc tcgaggatct cctgcactcc ggtgccggcc ttggcggagc aggggagggc
     1381 gttctccgcg gagatcccga tgatgttctc gatctgctcc ttcatccgct ccggctccgc
     1441 cgaggcgaga tcgatcttgt tgatgaccgg gatgatgtgc aggtggtggt cgaccgccat
     1501 gtacgagttg gccagggtct gcgcctcgac cccctgggtg gcgtcgacca ccagcagcgc
     1561 cccctcacag gccgagagcg atcgcgacac ctcgtaggcg aagtcgacgt gtccgggcgt
     1621 gtcgatgagg ttgaggcggt aggtcttccc gtcgcccgac ttgtactcga gcgccacggc
     1681 atgcgccttg atggtgatgc cgcgctcccg ctccaggtcc atgttgtcga gcacctgctc
     1741 gtccatctcc cgcatcgaca gcgcgccgca cagctccagg atccgatccg acagggtggt
     1801 cttgccgtga tcgatgtggg cgatgatgca gaagttgcgg atgagcgacg cgtccatgtc
     1861 ggagacctga ggagagtcct acagctcgag accgggctcg gcgttgaggt ccagggaggc
     1921 ggcgcggccc tcgaggagcc ggcgccggcc cacctccgcg atcatcgcgg cgttgtccac
     1981 cgccagatcg cgccgcaccg agtacatcgc cagcccgtgc cgcttcgcct cctcggcgac
     2041 gcggcggcga agctccgagt tgcaggcgac cccgcccgac aggcacaccg cccgggcgcc
     2101 cgcctcgtcc gccgccgtca tcaggcgatc gacgaggaac gccaccaccg acgcctggaa
     2161 tgaggcgagc aggtcgcggg tctcctggga gggggcgtcc tggtccgggg ccgggtaggc
     2221 ccgcgtcagg ccgcgcgcct gggcgtgccg cagcgccgcc gtcttgaagc cgctgaagga
     2281 gaaatcgagg ctgccgtcgc tcatcttcgg cggcgacagg cggaccgcct tcgggtcgcc
     2341 gctcggcgcc agccggtcga ccaccggccc gccgggatac ggaagcccaa gcatcttcgc
     2401 caccttgtcg aatgcctcgc cggcggcgtc gtcgcgcgtg cgcgcgatgc gccggggggg
     2461 ctcgtccccc tcgatgaggt agagaccggt atggcccccc gaggcgacca ggttcatcga
     2521 cggggagggg atctccccgt gctccagcca gacggagcgc aggtgccctt ccaggtggtt
     2581 cacccccagg agcggcagtc cccgccccca ggcgatcgcc ttcgccgcct gcaggccgac
     2641 gagcaggcac cccacgagcc ccggtccgta ggtgaccgcc acgccgtcga gatcggcgag
     2701 cccgcagccg gcgcgttcga gtgctgtgtc gatgaccagg tcgatctgct ccaggtgatg
     2761 gcgcgaggcg agctccggca ccaccccgcc gttcggagcg tgcagctcgg tctgcgaggc
     2821 gatgacgttc gacaggatcg tcgagccgcg gagcaccgcc gcggcggtct cgtcgcagga
     2881 gctctcgatc ccgaggagga gcggcccgga cgaagggggg gccgtcatct caggaggccc
     2941 cgccggcgcg gccggcgccc ccgcacaggg aagcgaggct cgcttcatac ggcgcccgcg
     3001 ccaccccgtt ctccgtgacg atcgccgtca ccagcgcgtg cggcgtcacg tcgaacgccg
     3061 ggtgcaggac cggcacgccc cgcggcacga cccgcttgcc tgcgatctcg accacctcgt
     3121 cggcggagcg ctcctcgatc gggatggagg cgccggtcgc gaggctcaga tcgacggtcg
     3181 aggtgggggc ggcgacgtag aacggcacct cgtgggcgcg cgccagcacc gcgacctggt
     3241 acgtcccgat cttgttcgcg acgtcgccgt tcgcggcgat ccggtcggca ccgacgatgc
     3301 aggcgccgat cgccttcttc tggaacaggt ggcccgccat cccgtcgcac aggacggtga
     3361 ccgggatgcc gtccttctgc agctcccatg cggtcagccg tgccccctgc aggaacggcc
     3421 gggtctcgtc ggcgaacacc gcgatccgct tgccggcttc gaccgcggca cggacgacgc
     3481 cgagcgccgt gccgtagccg gcggtcgcga gcgccccggc gttgcagtgc gtcaggaccg
     3541 ccccctcgcc cggcaggagc gcagcgccgt gccggcccat gctgcggttc gccgcgatgt
     3601 cctcggcgtg gaccgcgcgg gcctcggaca gaagcgcggc cgcaaggggc cccgccccct
     3661 cgtgccggtg cgcctcgaag cggcggcgca tccgcttgat cgcccagaac aggttcacgg
     3721 cggtgggacg cgtccccccc agaagcgccg cgagcgcctc gaagcgggcg gacgggtcct
     3781 ccgtcccagg gacgcgcacc ccgggctgga agccgagggc gattccgaag gcggcggcga
     3841 cgccgatcgc gggggcccct cggatcgcca tgccgcggat cgcctccgcg acctcctccg
     3901 gcgtcctcag ggtgaggtag atctcctcct ccgggagccg ccgctggtcg aggagcagaa
     3961 cccccccctc gccccactcg atcgttttga acattgggcc ggatgttagc gtgtgtgggc
     4021 cggtggcgca atcaatcgtg cgccggccac gggacccccg aaccgacccc atccgggtcg
     4081 cagtatggtg gcgcctctgg ggcaggctcc gggcggtatc cggggccccg cggccgccaa
     4141 gaaaccattg acagacaagc ggtttcaccg tatcaatcgg cgaaccgaat gacaccgctc
     4201 tggcggacgg atccgtaagg tcgctcgggc gtctttctgt cctgatcgcg ggatggaggg
     4261 cgccctttgc cattgttgga ggcgcgaggg agccccggat gatggaacgc tcacggtctg
     4321 gaccttcgtt caccagccgc atccggggaa tagcgctgcg gctcctggcc accggcgggc
     4381 tcgcggccct cgtgctggcg accctcacgc cgatccgggc cggccgaggc gcggccgagg
     4441 cgccgggtta cgaccggacc cagcgcgatt cgtccgaggc ctttttccgg cgcaatcgcc
     4501 tgccccatgg ccaccccagg atcgaggggc tgagcccgtt cgggacccag atccggggct
     4561 tcgccgggcc ggccgagggg tcgagccggg tgtccacggc cgccgggttc ttcgacctga
     4621 cccgcccgga ggagatcgcc tccctcccgg cccccctccg cctgcgctcc gaagtccccc
     4681 tccccggggg tcgcgggggg cttgtggccg gcatcgacct ggtgcaggtg cgcgccgcgg
     4741 tgctccaggg ccggggcgaa tcggggatgc gcctgctgct cgagccctac ggccgggtgc
     4801 ttggcctcgt cccggagcgc ggtttcatcg tcgaggtgcg cgatcgggcc gcggcggagg
     4861 ccctggcgcg cgagccgttc gtcgacgccg cgagcccgta ccacccggcc ttcaaaatcg
     4921 acccgaacct gggtcgtaca cccttcatcg aggcgaagcg ggcggcgagc ccgatcctgc
     4981 gcctccaggt gatcgcctgg caggccctcg acgcctccgg gcgcgatgag ctgcgcgccg
     5041 gcctgtcccg tctgctcggg ggcggggcgg tgcggatcga cggggacgac cgcctgctct
     5101 atgtcgaggc ggaggcgtcg aaggtcgccg ccatcgccgc gctgcccggg gtcgaggcgg
     5161 tggcggagga gccggagctg atgctcagca acgccgaggc gccgagcgtc gtgatggtcg
     5221 gaagcgtcga ggacaccctc ggggcgcgcc cgtaccacga cctcggagtc gacggcggcg
     5281 ggatcgacac caacggcgac ggcgcgcgcg tcaacgacgg caccgacagc gtgccgccgc
     5341 agatcgtcgc ggtcaccgac aatggcatca gcatcgacac cccgtccttc tcgcagaccg
     5401 cgacccagac gacgaccatc acgagcccgg tcggagccaa gcaccggaag gtgcacgcca
     5461 tccaatcggt ggccgacgcg ggggacaact gcgacggcgt cctgtccggc tccgggtcgc
     5521 acggcaacat cgtcgcctcc gccatcgccg catggccgtc ggcgctcggc gtctacgcca
     5581 ccaagacgac cttgccgcgc aacccgatcg tctcgggcat cgcgctggac ggcgtcgcgc
     5641 gcggcgcgcg catcatcatg caggacgccg ccaacttcac gcgctgcacc atcgacgaac
     5701 tgatcgagca gggggggaac atcagcgcgg ggaacgtgac cacgcgcctg acccagggca
     5761 tctgtccgaa gagcggcgga accgggccca tctgcacggg cgtctccggc ggcggcagcg
     5821 agacgcacct gcacgtcatg ccgttcggcg tgccgaactt cgacaacatc ctggacaacc
     5881 cgcaaaacgg ggcgtactcg gtcgaggcga gccagatcga caccttcttg gtgaacaacc
     5941 gggacttcat ggtcttcacg ccgatcggca accacggcgc caacccgaag aacctgacgc
     6001 agcggcgcat ccccgacctg ttcgacggca ccgccctcga caatgatccc aacttcccct
     6061 cggcgaacca gatcaacccg cccgcgaccg ccaagaacgt cgtcagcgtc ggcagccacc
     6121 gcaccgacat gcagaccttc gccgggacct tcaacgaaga ggaggtctcc tcgccgtggg
     6181 cctcgcgcgg ccccgcgacc aacgcctcgc tgcgcatggc cccgatcgtc acctccatcg
     6241 gggaggactt cagcggcgtc ttcggcgccc ccggcatcgg cggcgtggcc gccttccgga
     6301 gccgcgacaa cgacagcacc gcgccgatcg acgcgcagct cgacgagctg aacttcggga
     6361 cgtcgtacgc cgccgcgtac gccggcggag cgggggcgat cgtccgcgac tacttcgcgc
     6421 aggggttcta cccgggcggc acgcggcgca ccgccgaccg gatgtcgaac gtctccgggg
     6481 ccctggtgaa ggcggcgctg gtcgcgagcg ccaacttcct cgaggagaac ggccggagcc
     6541 agtacccgac cctcaccgac cgcttcgtcg cgcaggcgcg cgccggcaac gtcggcatgg
     6601 tgtcgggggt gaacgtcggc gtgctgggga acatggaaca gggatacggc cgcgtgcagc
     6661 tctcgaacgt cctgccgatc ccgaactggc cgaccgtgat cggcatcggg aagccggaca
     6721 cgatcgagca ctccgccgcg gcgctcctgg tgtgggacga catcggcacc ggcgagccgc
     6781 cgatcaacaa cacgacgacg gtcatcgagc acaccttcct cgtcgacgct ccgaacacgc
     6841 tcgtcctggg gggcggcggg agggccgtct ccctcggggc gctgcgcatc gcgctggcct
     6901 ggatcgatcc ccccgggacg gcactcgggg acggggccct catcaacgac ctcgacctcg
     6961 aggtcgaatc gcccgggccg gacggatccc tcgccaccac ggccgacaac ctgatctacg
     7021 acggcaacct ctacaacctc gggctcggtc cgcgggccgg gcagtggtcc gctccacgcg
     7081 tcgccgccga cctcgccgac ctgcgcaacc cggtcgaggc ggtgcacatc ccctccgacc
     7141 tcaacggcga cgggaacccg gccgactcgc gactctacgc cggcacctgg cgggtgcgcg
     7201 tcaagcgcgg cgtcggcggc gccgtgcccg ggagcatcac ccgcatcgac gggccgtcgg
     7261 aggacgccaa cggcaacttc cggctcgaaa ccctgcccct cccgagcgag gacctggacg
     7321 ccgacggcct gctcgacgcc aatgggcagc cgttcgcgct ggtcgtctcg gggccggtga
     7381 tggggaccgg gacgcagacc tggggcgggg cgccgcacag cctgccgcag agcgccacgc
     7441 acctcgacca gccgacctac ggctgcgccg acgatctggt ggtcaacgtc ttcgacccgg
     7501 acggcacgat cgccggcatc ggcgccgcca ccaccctgac ggtgcaggac gcgctcggga
     7561 acgtgctcga ctccgaacag gggttcacgt tcaccgaggc gccggcgggc agccacggat
     7621 tccgctccgc gaagatcccg gtgcgggtca tcgcgccgac gccggtgtcc aacaacggga
     7681 tcctcgagac cgacaccggc cagttcatcg tcgccgacta cgccgacagc ccggtgcctg
     7741 gacaggcgcg cgccaccgtc gcctgcaacc cgagcctgtt cgccggcgtc ctgcagatcc
     7801 gcgacgaggc tgacggcgcg gcgctgttca ccggcggctg cgatcgcgac cagtacccgg
     7861 acgcggggga gagcctgacc tacacggtcg cgctgctcaa cgccgaccgc ggcgacgact
     7921 acaccgacgt gacggcgacg ctggtgccgt ccggtctggg cgcggcggcg atccaggtgc
     7981 tcgactcgcc gaaggcgatc ggccggctcc cgggggggca gacggccggg atcagcttct
     8041 cgctgcgggt caacgcgacg acgctcaacg ccctgccggt cgccagccgc atcgccacac
     8101 tgaccctcac cctcgattcg acgtcgcgcc acaaggtgat cggccggcag tcgttcgcct
     8161 tcacgcacgc gctgaactcc gacaaggaga ccttccacta ctccaccgac tacccggcgg
     8221 gggggcgcga gatccgcgac ctgaaccgta atctccagat cgacaaggcc gatcagatcg
     8281 atccgttcac cggcatcgag gtgccggacg aggacctggt cttctcgacg ctgtggctca
     8341 gcgacggcgg ggtggtgcgg aacatcctcg gcgaggacct cgacaacgac ggtgtccgcg
     8401 acccgaacga ggcggacatc attccgaaca acggcctgct ggacaaaggg atcctggcgc
     8461 tcccgggcgg gccttcggcc aacgacaagg tgccgttctc cttcgacgcc aacaacggcg
     8521 ggttctaccc cgtccgtcac ccgaacagcg aggcgggggc gggaggcacg caggtggtct
     8581 gggagttcca cgcggacggg ctgtgcggct tccagaccgc caacccggac ggcgacggct
     8641 ccccccgttt ccagaacaac ggggccggca tctggcacac cggggatggc gacaccttca
     8701 cccccgacga gttctcgacc ggctgcgaca actacttcat gccgcagaac ctggccacgc
     8761 cggcgcaggc ggagttcatc ttcgacatgc tcgagtcacc catcgtcgcc aaggtgcacc
     8821 agacgaccga cgcgcgcggc ttcccgtaca ccgtggagtt ccagcgcctg gcgatgaatc
     8881 tcaaccatca gaccatcgac gcgtacgccg gggggcaggt caacctcgac tccgacgtgg
     8941 acagcgacga tcggaactgc ctgctctgcc aggtcttcta tccgcggttc ggcggggcgt
     9001 actacggcgt cgcgcgcttc aacacgtacg agaacggcgt cgatcagaat ggagcgggag
     9061 aggtccggca gcgaaccttc ggcccgaaag tcgatcccga caactcgatc aacgcgttcg
     9121 gatacgtctc cggagacgag accggcttct ccgggttcac ctccgacagc aacccggtct
     9181 cctcgagccc gatcccgacc gcgccgccgg atctgctgcc gtacccgatc ccgggtggcc
     9241 cgctgccgct cgcctccgac ggcaccccgg cgctcaacaa cgtccgcggc ccgacgcgca
     9301 acttcgagct gtcgctcgtc aactaccagg actcctacgt ctacttcccg accggccccg
     9361 gggcttacga gccgggcggg tacttcaacc ccggtccgac cggcaaccgc tggatgttcg
     9421 gcatcggctt cttcgtgatc gagagcacca ccctcctcaa cgactacggc ctggcggtgg
     9481 acgacgcggt gctggaatgg gacgaattcc acccgctcga cgagtcgcag ttcgtccccg
     9541 cccatacccc cgcctgtcag cggttcgggc agccggggca gccggccgga cagcagtgcg
     9601 ccaccatcgt cctcgatcgc accagcctct acgagtgcga cgagtcggtc agcgtgaccg
     9661 tccacgacgc gaagaaggcg ggccagggat cggtcgtcgt gcaggcggcg tccgacagcg
     9721 acggcacgcc gttcttcgcc gccaacggca ccggcttcct gccggcgaag agcttcacgc
     9781 ttccggaggt cacgccgggc gtgttccgcg gcaacatcac cgtcacctcg cagttcaaca
     9841 acgccgcgac cgtgttcgtg acgccggcga gcgaccagat cctcagcgtg tactacaacg
     9901 acccgctctg cgacgccgac ggcgacacgc aggtggcgga gtcggcgttc gacaacatcg
     9961 acggcgacgg gatcgcgtcc gcgtccgaca agtgccccgc ggtctacgac ccggcccagc
    10021 cggacgccga cggcgacggc cggggcgacc tgtgcgacaa ctgcccggcg ctggcgaacg
    10081 ccacgcaggt ggactccgac gcggacggga tcggggacgc ctgcgacttc gacgacgtgg
    10141 acgtggacgg cgtcgccaac gaggtggaca actgcccgga cgtctacaac ccgctgcaga
    10201 ctccgggagc ggcggggcgg gggacggcct gcgatcagac cacggatcgg gacggcgacg
    10261 gggtgaccga tcgcaacgac aactgcgtgc ggacgtcgaa cgccaaccag ctgaactccg
    10321 acaccgactt cctcggcaac gtctgcgacg gcgattgcgc gtcgccggcg gcgacgaacg
    10381 ccaccggcgg cgtctgccag cgcaccaaca cgacgatctg cacgacgaac gccaactgcc
    10441 cgaccaccgg gacctgcaac atcacctcct cgactgtctg caccaacaac ggccagtgcc
    10501 cgcaggggga gcagtgcatc ggcatcgtgc aggagacctg catgcgcgcg
//
LOCUS       DASRIQ010000257         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1926661, whole genome shotgun sequence.
ACCESSION   DASRIQ010000257 DASRIQ010000000
VERSION     DASRIQ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926661"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VFD06_06375"
     CDS             <1..938
                     /locus_tag="VFD06_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZN03197.1"
                     /translation="HAHLMVRDGEEIHAGDVLAKIPRATTKTKDITGGLPRVVELFEA
                     RRPKEPAVISEIDGVVRYGEVVKGKRGLVVSDDETGEVKEYEVPKGVHINMQENERVR
                     AGEPLIDGPRDPHDILRVKGKEDLQQYLVNEIQEVYRLQGVNINDKHIETIVRQMMRW
                     VKIEEVGDTEFIVDEQVDKFRFQSENERVIGEGGQPATGRPLLLGITKASLSTDSFIS
                     AASFQETTRVLTEAAIAGKVDYLRGLKENVIMGRLIPAGTGLDVYRSIQVAPDGPPPM
                     EEILPEGLEGTGLEEGLDQRTVEEIISARQDKGVA"
     gene            1136..1510
                     /gene="rpsL"
                     /locus_tag="VFD06_06380"
     CDS             1136..1510
                     /gene="rpsL"
                     /locus_tag="VFD06_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZN03198.1"
                     /translation="MPTVNQLVRIGRERLRGRTKSPALISCPQKRGVCTRVFTSTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGVGHNLQEHSIVLIRGGRVKDLPGVRYHVVRGTL
                     DSMGVEGRKQSRSKYGAKRQKS"
     gene            1601..2074
                     /gene="rpsG"
                     /locus_tag="VFD06_06385"
     CDS             1601..2074
                     /gene="rpsG"
                     /locus_tag="VFD06_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZN03199.1"
                     /translation="MPRRAMVAKRETEADPIHGSPLVTKFINCLMTQGKKSTAQAIFY
                     GALEQIREKTKDDPVKTLKKAVDNVKPLLEVKSRRVGGATYQVPVEVNPARRQSLGIR
                     WLLLYATQRGSEKQMLNKLANELLDASNNRGGAIKKKEDTHKMAEANKAFAHYRW"
     gene            2089..>2267
                     /locus_tag="VFD06_06390"
     CDS             2089..>2267
                     /locus_tag="VFD06_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZN03200.1"
                     /translation="MARPHPLDRYRNIGIVAHIDAGKTTTTERMLFYTGKIYKLGEVH
                     DGTATMDWMVQEQERG"
BASE COUNT          446 a          704 c          805 g          312 t
ORIGIN      
        1 cccacgcgca cttgatggtg cgggacggcg aagagatcca cgccggtgac gtgctggcga
       61 agatcccacg tgcgacgacg aagacgaagg acatcaccgg cggtctgccg cgcgtcgtcg
      121 agctgttcga ggcgcggcgt ccgaaggagc cggccgtcat cagcgagatc gacggcgtcg
      181 tgcgttatgg cgaggtggtg aaggggaagc gcggtctcgt ggtctccgac gacgagaccg
      241 gcgaggtcaa ggaatacgag gttcccaagg gcgtgcacat caatatgcag gagaacgagc
      301 gggtgcgcgc cggcgagccg ctcatcgacg gcccgcgcga cccgcacgac atcctgcggg
      361 tcaaggggaa ggaggacctg cagcagtacc tggtgaacga gatccaggag gtctaccggc
      421 tgcagggcgt caacatcaac gacaagcaca tcgaaacgat cgtccggcag atgatgcgct
      481 gggtgaagat cgaggaggtc ggcgacaccg agttcatcgt cgacgagcag gtggacaagt
      541 tccgcttcca gtcggaaaac gagcgggtca tcggcgaagg gggccagccc gccaccggcc
      601 gcccgctgct cctcgggatc accaaggcgt cgctgtcgac cgactcgttc atctcggcgg
      661 cctccttcca ggagacgacg cgcgtgctca ccgaggcggc catcgccggc aaggtggact
      721 acctgcgcgg tctcaaggag aacgtcatca tgggccgcct gatccccgcc gggaccgggc
      781 tcgacgtgta ccgcagcatc caggtcgccc cggacgggcc gccgccgatg gaggagatcc
      841 tcccggaggg gctcgagggg accgggctgg aggaagggct cgaccagcgg accgtcgagg
      901 agatcatttc tgcccggcag gacaaggggg tcgcgtagcg gttccggccc cgatcctccg
      961 tctttcgtaa ggtttcggtg tagccccctt gacaggtccg ggggggcctc tggtagtatc
     1021 cgcgggtttc cggcttacag ccttgagtgt caaggcgccc ggaagtgcaa cggtgccttg
     1081 gagttgtgcg cggtcgcgcc cagcgcgacc gcgttgtcgt cacttcggag atctcatgcc
     1141 gaccgtcaac cagctcgtgc gcatcggacg ggagcgcctg cgcgggcgca ccaagagccc
     1201 ggcgctcatc tcctgcccgc agaagcgcgg cgtctgcacg cgcgtgttca cctccacgcc
     1261 gaaaaagccg aactcggcgc tgcggaaggt ggcgcgcgtc cgcctgacca acgggatcga
     1321 ggtgacgacc tacatcccgg gcgtcgggca caacctgcag gagcactcga tcgtgctcat
     1381 ccgcggcggc cgcgtgaagg atctcccggg cgtgcggtac cacgtggtgc gcgggacgct
     1441 cgactcgatg ggagtcgaag ggcggaagca gagccggtcg aagtacgggg cgaagcgaca
     1501 gaagtcgtag cgcggaagag agcgcgtgcg ggggcggacg aaggacggtc cgccccctgt
     1561 gttcgaggcg gcgaaggatg accgccggga gtgaggttcg atgccgcgaa gggcgatggt
     1621 ggcgaagcgg gagacggagg ccgatccgat ccacgggtcg ccgctggtca ccaaattcat
     1681 caactgcctg atgacccagg ggaagaagtc gaccgcccag gcgatcttct acggggcgct
     1741 ggagcagatc cgggagaaga ccaaggacga ccccgtgaag accctcaaga aggcggtgga
     1801 caacgtcaag ccgctcctcg aggtcaagtc gcggcgcgtc ggcggcgcca cgtaccaggt
     1861 gccggtcgag gtgaacccgg cgcgtcggca gtcgctcggc atccgctggc tgctgctgta
     1921 cgccacccag cgcggctccg agaagcagat gctcaacaag ctcgccaacg agttgctgga
     1981 cgcgtccaac aaccgcggcg gggcgatcaa gaagaaagaa gacacccaca agatggcgga
     2041 ggccaacaag gccttcgccc attaccgctg gtgagctgag acaaggccat ggcccgaccg
     2101 catcctctcg accgctaccg caacatcggc atcgtcgccc acatcgacgc gggcaagacg
     2161 acgacgaccg agcggatgct gttctacacg gggaagatct acaagctcgg cgaggtgcac
     2221 gacggcaccg cgaccatgga ctggatggtc caggagcaag agcgcgg
//
LOCUS       DASRIQ010000258         4530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3774976, whole genome shotgun sequence.
ACCESSION   DASRIQ010000258 DASRIQ010000000
VERSION     DASRIQ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3774976"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4317)
                     /locus_tag="VFD06_06395"
     CDS             complement(<1..4317)
                     /locus_tag="VFD06_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HZN03201.1"
                     /translation="MTAGPRRLLLSFVTLFTVVALPFVQRHELGADSCSPEAPPGNYG
                     TDLDGQGSRIDLGAPPALAGTVFTLEAWVRRQGPGLATSTGEVQAIPILTRGRDEAGS
                     EGNNLDLNWFLGLRASDGVLVADFEDDAIGANHPAAGTTAIPLHVWTHVAATYDGGTW
                     RLYVNGALETTLAVGATPRADALAHAAIGTAFDSAGAADGWFDGVVDEARVWSVARGD
                     GDILAGMAAAIASAAGLAGRWGLDEGIGTVALDSSGGLNHGTLSAGVSVRGTPFASQP
                     EALVDHALEFDGAAAHVSFGTAGPLGLATFTIEAWFNRRGTGIATSTGVDGVVAIPLA
                     AKGRDEGEGDATDLNWFLGLRAADGVLVADFEDAATGANHPVAGTTTVLSGRWNHAAA
                     VFDGATWRLYLNGILEAEAAAAFTPRSDSLAHAALGSALDSAGTAAGFFDGSLDEVRV
                     WSVARSGAEIQDGLKREITTGTGLVARYGLSEGTGASIADPDHPAAPVGTVVSGTWVD
                     GYPFADRLVVADDTCDGIDQDCDGTPDDGYVPVMCGTTDVGACALGTSVCTNGTIGCS
                     GNIEPTTEVCDGADNDCDGATDEALGTLTCGAGVCAASMPACAGGVPQTCIPGPPQTG
                     TSLSFDGVDDSVDFGNHASINTHGTQSFTVEEWFKATSFGPNAGIFRAGRQGSWSQVV
                     IQTPASAPFNRLTVSVESNSQEQIDTAPVEINPNQWYHVAAVIDRGAGEVRVYLDGNL
                     VATSDSSLWGSATIGDTFYSTVIGAARRSDETLGNFFSGAIDELRIWNQARTAQQIQA
                     HRFQAITTAPGLLARWGFDAGAGTQAVDSVSGGALNNGSLTNGTAWLNESGMGVESAC
                     DGLDNDCDGLVDEDDQSVPTICGSGVCVSTGVTSCVNGVVQDSCIPLPGAPNDASCNN
                     VDDDCDGLVDEDFVGQPITCGEGVCGATGTTTCAYGQPGVSCQPNRPAANYGLDLDGA
                     GSYVTMGHVPALGLQTYTVEVWFKRHGTGVGCDTGQQGLSEAVPLVSRGRGEADGGTM
                     DMNWFLGVGQTNGRLEADFEEYGTGLNHPVVGATPTAPDVWYHGAVTYDGSHMRLYLN
                     GVLEAEHFEQNAVPRYDSAQHVAIGSALNTLGQPSGWFDGVIDEARVWNYARSAAEIQ
                     ADLGHPVLSSPGLVARWALDDTAGSAVHDSAGTANGTLTSGSFVTGTPFAIGPSPNPD
                     HAVALDGRVSRIVIPPTPALGLQTFTIETWFNRKWLGDYTDTGSGGLEAVPLIAKGRG
                     EAEGDNRDINYFLGLRYSDSRLMADFEDMGGGNHPLQGTAAIPDGTWNHVAAVYDGAS
                     WRLYLNGRLEAIQQGVTATPRFDSIQPIGFGSAFTSEGSPGGFFAGEMNEVRIWSVAR
                     TQTQIQDGMFEQIASAPGLVGRWALTENTGTLVADTAGLPAHDGTLESGAWADGYPFN
                     NKP"
BASE COUNT          614 a         1569 c         1646 g          701 t
ORIGIN      
        1 cggcttgttg ttgaacggat agccgtccgc ccaggcgccg ctctccagcg tgccgtcgtg
       61 ggccggtaac ccggcggtgt cggcgacgag ggttccggtg ttctccgtca gcgcccagcg
      121 tcccacgagc cccggagcgg acgcgatctg ctcgaacatc ccgtcctgga tctgcgtctg
      181 ggtgcgcgcc acactccaga tgcgcacctc gttcatctct cccgcgaaga acccaccggg
      241 agagccctcg gaggtgaacg cgcttccgaa gccgatcggc tggatgctgt cgaagcgcgg
      301 cgtcgccgtc acgccttgct ggatcgcctc caaccggccg ttgaggtaga ggcgccagga
      361 ggcgccgtcg tacaccgcgg cgacgtggtt ccaggtgccg tccgggatcg ccgccgtgcc
      421 ctggagcggg tggttccctc cgcccatgtc ctcgaagtcg gccatcagac ggctgtcgct
      481 gtaccgcagc ccgaggaagt agttgatgtc gcggttgtcg ccttcggctt cgccgcgtcc
      541 cttcgcgatg agcggcacgg cttcgagccc gccggagccg gtgtcggtgt agtccccgag
      601 ccacttccgg ttgaaccagg tctcgatggt gaaggtctgg aggccgagcg cgggggtcgg
      661 cgggatgacg atccgcgaga cgcgtccgtc cagcgcgacc gcgtggtcgg gattggggga
      721 cggcccgatc gcgaacgggg tgccggtcac gaacgaaccg gacgtcagcg tcccgttggc
      781 cgtcccggcc gaatcgtgga cggccgagcc ggccgtgtcg tccaacgccc agcgcgccac
      841 caggcccggg gaggagagaa cggggtgccc caggtccgcc tggatttccg ccgcggagcg
      901 cgcgtagttc cagacgcgcg cttcgtcgat gacgccgtcg aaccagccgg acggctgccc
      961 gagcgtgttg agggcgctgc cgatggcgac gtgctgcgcg ctgtcgtagc gcgggacggc
     1021 gttctgctcg aagtgctccg cctcgagcac gccgttcagg tagagccgca tgtggctgcc
     1081 gtcgtaggtg accgcgccgt ggtaccagac gtccggggcg gtgggggtcg ctccgacgac
     1141 cgggtggttg aggccggtcc cgtattcttc gaagtcggcc tcgaggcggc cgttggtctg
     1201 gccgacgccg aggaaccagt tcatgtccat ggtgccgccg tccgcctcgc cgcggccgcg
     1261 gctgacgagc ggcaccgcct ccgagagccc ctgctggccg gtgtcgcacc cgacgcccgt
     1321 gccgtgacgc ttgaaccaca cctcgacggt gtaggtctgc agcccgagcg cggggacatg
     1381 ccccatggtg acgtacgagc cggcgccgtc gagatcgagg ccgtaattgg ccgccggccg
     1441 gttcggctgg cagctgacgc cgggctggcc gtatgcgcac gtcgtcgtcc cggtggcgcc
     1501 gcacaccccc tcgccgcacg tgatcggctg cccgacgaag tcctcgtcca cgaggccgtc
     1561 gcagtcgtcg tccacgttgt tgcacgacgc gtcgttcggc gcccccggga gcgggatgca
     1621 actgtcctgg acgaccccgt tgacacagga ggtgacgccg gtcgagacgc acacgccgga
     1681 gccgcagatg gtcgggacgg actggtcgtc ctcgtccacg agcccgtcgc agtcattgtc
     1741 gagcccgtcg caggccgact cgacgcccat gccggactcg ttcagccatg ccgtgccgtt
     1801 ggtcagtgag ccgttgttga gcgcgccgcc gctgaccgag tccaccgcct gcgtcccggc
     1861 gccggcgtcg aagccccagc gggcgagaag ccccggcgcc gtggtgatcg cctggaagcg
     1921 atgcgcctgg atctgctgcg cggtgcgcgc ctggttccag atgcgcagct cgtcgatggc
     1981 tccggagaag aagttgccca gcgtttcgtc ggaccgccgc gccgccccga tcacggtcga
     2041 gtagaaggtg tcaccgatgg tggcgctgcc ccagaggctg gagtcggacg tcgccacgag
     2101 attgccatcc agatagaccc tcacctcgcc ggcgccgcgg tcgatgacgg cggccacgtg
     2161 gtaccactgg ttcgggttga tctcgaccgg cgccgtgtcg atctgctcct gcgagttgct
     2221 ctccaccgat accgtcaggc ggttgaacgg cgccgacgcc ggtgtctgga tgacgacctg
     2281 cgaccaggat ccctgccggc cggcgcggaa gatgccggca ttcggcccga aggaggtcgc
     2341 cttgaaccat tcctccaccg tgaagctctg cgtcccgtga gtgttgatcg aggcatggtt
     2401 gccgaagtcc acggagtcat ccacgccgtc gaacgacagg ctcgtcccgg tctggggcgg
     2461 tcccggaatg caggtctgcg gcacgccgcc ggcacacgcc ggcatgctcg cggcgcagac
     2521 gccggcgccg cacgtcaggg tgccgagggc ttcatccgtg gcgccatcgc agtcgttgtc
     2581 ggcgccgtcg cagacttccg ttgtgggctc gatgttcccg ctgcagccga tcgttccgtt
     2641 ggtgcagacc gaggtgccga gggcgcaggc gccgacgtcg gtcgtgccac acatcactgg
     2701 gacgtagccg tcgtcggggg tgccgtcgca gtcctgatcg atgccgtcgc atgtgtcgtc
     2761 ggcgacgacg agccggtcgg cgaacggata gccgtccacc caggtcccgg agaccaccgt
     2821 gccgaccgga gcggccgggt gatccgggtc ggcgatcgag gcgccggtcc cttcgctcaa
     2881 cccgtagcgc gcgaccagcc cggtgccggt ggtgatttcc cgcttcaggc cgtcctggat
     2941 ctccgcgccc gagcgcgcca cgctccagac gcgcacctcg tcgagagagc cgtcgaagaa
     3001 tccggccgcg gtcccggcgg agtcgagggc gctgccgagc gcggcgtgcg ccaggctgtc
     3061 ggagcggggc gtgaacgcgg ccgccgcttc cgcctcgagg atgccgttca ggtacaggcg
     3121 ccaggtggcg ccgtcgaaca ccgcggcggc gtggttccag cgtcccgaca ggaccgtggt
     3181 cgtcccggcc accgggtggt tggcgccggt ggcggcgtcc tcgaaatcgg ccacgagcac
     3241 gccgtcggcg gcgcgcagcc cgaggaacca gttgaggtcg gtcgcgtccc cctccccttc
     3301 gtcgcgccct ttggcggcga gcgggatcgc cacgacgccg tcgacgccgg tgctggtcgc
     3361 gatgccggtg ccgcgtcggt tgaaccaggc ctcgatcgtg aaggtcgcaa gaccgagggg
     3421 gcctgccgtg ccgaagctga cgtgcgcggc ggcgccgtcg aactccagcg cgtgatccac
     3481 gagcgcctcc ggctgcgacg cgaacggcgt gccgcggacg gacaccccgg cgctgagcgt
     3541 gccgtggttc aggccgccgc tgctgtcgag cgcgaccgtg ccgatccctt cgtcgagccc
     3601 ccagcggccg gcgagaccgg ccgcgctcgc gatcgccgcg gccatcccgg ccaggatgtc
     3661 gccgtcgccg cgggcgacgc tccagacgcg cgcctcgtcc acgacaccgt cgaaccagcc
     3721 gtccgcggca ccggcggaat cgaacgcggt gccgatcgcc gcgtgcgcca gggcgtcggc
     3781 gcgcggcgtc gccccgaccg cgagggtcgt ctcgagcgcg ccgttgacgt acaggcgcca
     3841 cgtgccgccg tcgtaggtcg cggcgacgtg cgtccagacg tgcagcggga tcgccgtggt
     3901 gccggcggcc ggatggttcg cgccgatcgc gtcgtcctcg aagtcggcca cgagcacgcc
     3961 gtcgctcgcg cgcagcccga ggaaccagtt gagatcgaga ttgttccctt ccgagccggc
     4021 ctcgtcgcgg ccgcgcgtga ggatcgggat cgcctgcact tcgccggtgc tggtcgcgag
     4081 ccccggcccc tgccggcgca cccacgcctc gagcgtgaag acggtgccgg cgagcgccgg
     4141 cggggccccc agatcgatgc gcgatccctg gccgtcgaga tcggtcccgt agttgccggg
     4201 aggggcttcg ggcgagcagg aatcggcgcc gagctcgtgg cgctgcacga aggggagcgc
     4261 gacgacggtg aacagggtga cgaaggacag aagcaggcgg cgggggcctg cggtcatgtc
     4321 gtggctcctt caagctgaag ccggcggagg gagagccgac gggggaggga ccgaaggtcc
     4381 ggcatcttca ttcagttgtt gtcgatgttc gcgctgcgac atgacccggg gacggggccc
     4441 ggtccaccct gcgcgtgact gggatctacg atgatttcag gcggatgcta cggacagttt
     4501 cgagatcgcg gggagctggc catcactcgc
//
LOCUS       DASRIQ010000259         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2733219, whole genome shotgun sequence.
ACCESSION   DASRIQ010000259 DASRIQ010000000
VERSION     DASRIQ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2733219"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFD06_06400"
     CDS             <1..459
                     /locus_tag="VFD06_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03202.1"
                     /translation="QRAETRDDVFAVILDFVAARFRRTALFVVQGDRVTGWSGRGDGI
                     EPIRVRQVSVPLDRPSLFAFFRGGADDYQGPVSDLPANTRFYLDLGCPAPERALLLPL
                     RIKDRPAVILYADNAGERGPSPDIATLRRLLAKASLALEILILKTKILTL"
     gene            456..1052
                     /gene="amrA"
                     /locus_tag="VFD06_06405"
     CDS             456..1052
                     /gene="amrA"
                     /locus_tag="VFD06_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HZN03203.1"
                     /translation="MSDLPPGSLLTPEEGDLLLKLARRAVQAAIEGGDVPEEADGRPW
                     LRARRDVFVTLRLQGALRGCLGTYGSPGTLLENLVQAARGAARDDARFSPVAPHELRG
                     LRIELTLLDPPFAIAGPEAIALGRHGIRVSQRHARGLLLPQVAIEHRLDAEAFLGLAC
                     RKAGLRPDAWRHGARIEAFTAECVSEPEALPGPRAGPA"
     gene            complement(1049..2440)
                     /locus_tag="VFD06_06410"
     CDS             complement(1049..2440)
                     /locus_tag="VFD06_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03204.1"
                     /translation="MPCRALRLVAGGETMPISKNAVLLMSAVLALVLCLPGIVPAAEP
                     DGAAAGSGTVNTVPPETPTAPPKNLRKVGDHWTPWAPPDPESFPPGATVHVIVPGETL
                     WDLSDLTYNNPYLWPQLWNENRYILDSHWIYPGDPLLLPPRPMVVSGPGAVPTEIVPQ
                     DQEGAPHTDLGPMQDGSEGPEGPLQAETPAQPGPSTPAHGQGDGFSSGRLVDWDDLRC
                     SGYITEDSKRGELFIAENEEPGYQTVTLGSLVYLNKGKKDERLQPGAKFTIVEHEGKV
                     LHPITDRTQGIYTKRLGELRVLKVLDDTALATVTFACDEMRVGDELETIDLKEVPQGP
                     VPPFDRLRLERNGKPTGYVIHTKDLAVRVATGDIVSIDLGSEDGISPGVYLTAFAAVG
                     KDRLGHMPEYHFKYQNEIFTSADLHWDEGHDEFPSLPIAQIIVMTTEPHTATAKVIYS
                     LREMSVGTMVEVD"
     gene            complement(2519..>3139)
                     /locus_tag="VFD06_06415"
     CDS             complement(2519..>3139)
                     /locus_tag="VFD06_06415"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03205.1"
                     /translation="GDDAGARDAFEAALAIDARFGAARAGLALLRLRSGDEAGALEEW
                     ERAERSSPLPPESLAARAALHAARRRLDLAIEDYRRALRLAPGRADRRADLALLLLAR
                     GLSDQARAEARHAVETAPGSCRARLAEARVLRAAGDGPGALAAARAAFATDPACADAR
                     VEAARILLQAGRQSEAAAEVASALAAGTDPAVFSVDPDLRDLLPGR"
BASE COUNT          422 a         1140 c         1116 g          461 t
ORIGIN      
        1 cagcgcgccg agacccgcga cgacgtcttc gcggtcatcc tcgatttcgt cgcggcgcgg
       61 ttccgccgca ccgcgctgtt cgtcgtgcag ggggatcgcg tgaccgggtg gagcggacgc
      121 ggcgacggga tcgagccgat ccgggtgcgg caggtgagcg tgccgctcga tcgaccgtcc
      181 ctcttcgcgt tcttccgcgg gggcgccgac gactaccagg gaccggtgtc ggacctgccc
      241 gcgaacacgc gcttctacct cgacctcggc tgccccgcgc cggagcgtgc cctgctcctg
      301 ccgctgcgga tcaaggaccg gccggcggtc atcctctacg cggacaacgc gggcgagcgc
      361 ggcccttctc ccgatatcgc caccctgcgg cgcctcctcg cgaaggcgtc gctggcgctc
      421 gaaatcctca tcctcaagac caaaatcctg accttgtgag cgacctgccg cccggctccc
      481 tcctgactcc ggaggaaggg gatctgctcc tcaaactggc gcggcgggcg gtgcaggcgg
      541 cgatcgaagg cggggacgtc ccggaggagg cggacggccg cccctggctc cgggcccgcc
      601 gggacgtgtt cgtgacgctg cggctgcagg gagcgctccg gggctgcctc ggcacctacg
      661 gttcgcccgg cacgctgctc gaaaacctcg tgcaggcggc ccgcggggcg gcgcgcgacg
      721 acgcgcggtt ttctcccgtc gcaccccacg agctgcgcgg tctgcggatc gagctgacgc
      781 ttctcgatcc ccctttcgcg atcgcgggac cggaggcgat cgccctggga cgacatggga
      841 tccgcgtctc gcagcgtcac gcgcgagggc tgctcctccc ccaggtggcg atcgagcatc
      901 gcctcgatgc cgaggccttt ctcgggctcg cctgccgcaa ggcggggctt cggcccgacg
      961 cctggagaca cggcgcgcgg atcgaggctt ttacggcgga atgcgtgtcg gagccggagg
     1021 ccctcccggg gccgcgggcc gggccggctt agtcgacctc gaccatcgtc cccaccgaca
     1081 tctcgcgcaa cgaatagatc accttcgcgg tggcggtgtg cggctcggtc gtcatgacga
     1141 tgatctgggc gatcggcagc gacgggaact cgtcgtggcc ctcgtcccaa tgcaggtcgg
     1201 cgctggtgaa gatttcgttc tggtacttga agtggtactc cggcatgtgc cccagcctgt
     1261 ccttccccac cgccgcgaag gcggtgaggt acaccccggg ggagatgccg tcctcgctgc
     1321 caagatcaat ggacacgatg tcgccggtcg cgacccggac cgccagatcc ttggtgtgga
     1381 tgacgtaccc ggtcggcttg ccgttgcgct cgaggcgcag ccgatcgaac ggcgggaccg
     1441 gcccctgcgg cacttccttc aggtcgatcg tctcgagctc gtcgccgacg cgcatctcgt
     1501 cgcaggcgaa ggtgacggtc gccagcgcgg tgtcgtcgag caccttgagc acgcgcagct
     1561 cgcccaggcg cttggtatag atcccctgcg tccggtcggt gatcgggtgc agcaccttcc
     1621 cctcgtgctc gacgatggtg aacttggcgc cgggctgcag ccgctcgtcc ttcttcccct
     1681 tgttgagata gacgagcgag ccgagcgtga ccgtctggta ccccggctcc tcgttctcgg
     1741 cgatgaacag ctccccgcgc ttcgagtcct cggtgatgta cccgctgcag cgcaggtcgt
     1801 cccaatccac gagccggccg gaggagaacc cgtccccctg cccatgcgcc ggggtggacg
     1861 gaccgggctg cgccggagtc tccgcctgca gcggcccttc cggcccctcg gacccgtcct
     1921 gcatcggccc cagatcggtg tgcggcgccc cctcctgatc ctgcggcacg atctcggtcg
     1981 ggacggcgcc cggcccggag accaccatcg ggcgcggcgg aagcagcagc ggatcgcccg
     2041 ggtagatcca gtgagagtcc aggatgtacc ggttctcgtt ccacaactgc ggccacaggt
     2101 aaggattgtt gtaggtcagg tcggacaggt cccagagggt ctcccccggc acgatgacgt
     2161 gcaccgtcgc ccccggcggg aacgattccg gatcgggcgg cgcccacggg gtccagtggt
     2221 cccccacctt gcgcaggttt ttcggaggcg ccgtcggcgt ctcgggcggc acagtgttca
     2281 ccgtcccgct gcccgcggct gcgccgtcgg gctcggcggc cggcacgatc cccgggaggc
     2341 agagcaccag ggcgagcacg gcggacatca ggagcacggc gttcttggag atcggcatcg
     2401 tctctccccc agcgaccagc cgcagcgcgc ggcagggcat ggaccgctcc aaaaataggt
     2461 tccggagaga tgaagtcaag gcgggtggtc ggcgccgggg gcggttacgg gagtgcgatc
     2521 agcgtccggg cagcaggtcg cggaggtccg gatcgaccga gaacacggcg ggatccgtcc
     2581 ccgcggcgag cgcggatgcc acctccgcgg ccgcctccga ctgccggccg gcctggagca
     2641 gaatgcgggc cgcctccacc cgcgcgtcgg cgcaggccgg gtcggtggcg aacgccgccc
     2701 gcgcggcggc cagagccccc ggcccgtcac ccgccgccct gaggacccgc gcctcggcca
     2761 gccgcgcccg gcacgaacca ggggcggtct cgacggcatg gcgtgcctcg gcgcgcgcct
     2821 gatcgctcag cccgcgggcc aggaggagga gggcgagatc agcccgccgg tcggccctcc
     2881 cgggggcgag ccgaagggcg cggcggtagt cctcgatggc cagatcgagc cggcgccgcg
     2941 cggcgtgcag ggcggcgcgg gccgcgagcg actccggcgg cagcggtgag gaccgctccg
     3001 cgcgttccca ctcctcgagc gcccccgctt cgtccccgga acgcaggcgc aagagcgcca
     3061 gcccggcgcg ggccgcgcca aaccgcgcgt cgatcgccag cgccgcctcg aaggcgtcgc
     3121 gggcaccggc gtcgtcgcc
//
LOCUS       DASRIQ010000260         5417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3495026, whole genome shotgun sequence.
ACCESSION   DASRIQ010000260 DASRIQ010000000
VERSION     DASRIQ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495026"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFD06_06420"
     CDS             <1..268
                     /locus_tag="VFD06_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN03206.1"
                     /translation="IGATDTQIFMQFLAESAILSLIGGLLGVGAGIGVILAVTKYFPG
                     GLPVHLVAISISLGLSVLLGVLYGLYPALKASRLQPVEALRTAL"
     gene            284..1774
                     /locus_tag="VFD06_06425"
     CDS             284..1774
                     /locus_tag="VFD06_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HZN03207.1"
                     /translation="MRGAPVLVLLAALAWSAPAAAAPAADDPTRPLLWATDAEGGAPY
                     TFKDPKDPNRNIGFEVEIIAEIEKEIGQPIVAHQYAFSSLILGLQRGDFDFAMNGLEV
                     TPDRAEKVLFSVPYYVFREQLVVRKGEARFRDLEGCGTIDCVVGTLEDTAADRLLHAR
                     GIRATMYDGVVEPYRDLVLGRLDAVLLDLPIAIYYAKPNPDLEFAGEAMARGTYAIMF
                     QKGNEALKRRIDAALETILEDGRLRAILERWNLWDEEQAALAQVGSGDILSASRDAWT
                     FDRYFPLLLRGAVLTVFLAFVSFGLAILLGLPIACMRLYGPAPMRWVAIGYVEFFRGI
                     PVLLLLYVLYYGLADLSTASGLGSAFRLDPIHAAILGLGLNYAAYEAEIYRAGISAVP
                     YGQWEAAASLGMTGWLAFRRIILPQAVRTILPPMTGDFVALFKDTSIVSTIAVVELSK
                     QYQILSKSSMKYLEIGLLTAVLYLVMSVPLGFLSRWLERRWGRGIL"
     gene            1771..2535
                     /locus_tag="VFD06_06430"
     CDS             1771..2535
                     /locus_tag="VFD06_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZN03208.1"
                     /translation="MTANPGIDLTLEGLRKVYDGHTILDDVTLSIAAGTTVALIGPSG
                     GGKSTLLRCLNGLTPFEGGSVRVGEHRLGPGPVEGPNRAVAQQIRRLLGMIFQDFQLF
                     PHLTAIENLIEAPVRVLGSPAEAARAEADRLLDRVGLSHRKDAYPNMLSGGQKQRVAI
                     ARALAMHPRGLLCDEITSALDPELKGEVLAVVEDLRRDGITLVMVTHEIGFARRAADR
                     VLVLDHGAILEDGPPREVLERPKTARTRQFLSLLMG"
     gene            complement(2567..3208)
                     /locus_tag="VFD06_06435"
     CDS             complement(2567..3208)
                     /locus_tag="VFD06_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03209.1"
                     /translation="MRLAMGLVATMTALLLGLVTASARSSFDSQDASMRTNATNILTL
                     DRLLARYGPETRATRDLIRQAVAFRVEATWPEDGSAGAGLTQAVGTPAVEEIENQVLQ
                     LAPATDAQRWFKSEALRLTEDVMKARWRALGSQEGSVPLLFLAVVILWLTATFVSFGL
                     YAPPNASVITVLFVAAISVAAAVFLTLELDGPFDGLIKISSGPMRYALSQLGQ"
     gene            3549..4187
                     /locus_tag="VFD06_06440"
     CDS             3549..4187
                     /locus_tag="VFD06_06440"
                     /inference="COORDINATES: protein motif:HMM:NF022620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 family protein"
                     /protein_id="HZN03210.1"
                     /translation="MIRTGLAFVLALACCLPAMAAAAPDKAEKVASGMLKFDDVLTEG
                     TTRISGVLATMNSLSTATGSDLVSKYKTFTKQVNELDATAKKAKARSEDATSQREEYL
                     EQWQESQGQIQNEELKAASAARRAELMPKIEGIKEALTAASQGFAPFMQDLKDLTIFL
                     GNDLSEKGVATASELMAKCTEGGAKVNADIERGSAGVKDLAASISPTSAAKK"
     gene            complement(4218..5057)
                     /gene="speB"
                     /locus_tag="VFD06_06445"
     CDS             complement(4218..5057)
                     /gene="speB"
                     /locus_tag="VFD06_06445"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HZN03211.1"
                     /translation="MTRRQATSPGAGSVALVGVPLDENSSLLRGPAQAPGRIRETLFS
                     GSSSDCSEDGTVIIGSERFVDTGDLSLPQGAAAMQAIEAGVADLLARGARVLALGGDH
                     SITFPILQAHAARHPDLAVLQFDAHPDLYDDFDGNPHSHASPFARVLEKGLVKRLVQV
                     GVRSTNAHLRQQIARFGVESITMREFRPDLRLEFAQPVYVSVDMDALDPAYAPGVSHH
                     EPGGLSVRDILSVLEGTRGRIIGADIVEMNPLRDPVGTTAAVAAKLVKELAARLLRDA
                     VVS"
     gene            complement(5054..>5417)
                     /locus_tag="VFD06_06450"
     CDS             complement(5054..>5417)
                     /locus_tag="VFD06_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HZN03212.1"
                     /translation="NDGGAAAGHWISLKLVGTRSNKDALGAFVTLTAGERRQVREVRS
                     ASSYMSQNDMRLHFGLGTATRVDSIEIRWPNKAKTRETIGPQEADRFLVLREGAGVVA
                     SARAGQTIAAGAAKGPGR"
BASE COUNT          853 a         1903 c         1822 g          839 t
ORIGIN      
        1 gatcggcgcc accgacacgc agatcttcat gcagttcctg gccgagtcgg cgatcctgtc
       61 gctcatcggg gggctcctgg gcgtcggcgc cggcatcggg gtgatcctcg cggtgacgaa
      121 gtacttcccc ggcggcctgc cggtgcacct cgtcgccatc tccatctcgc tcggcctgtc
      181 ggtcctcctc ggggtcctct atgggctcta cccggccctc aaggcgtccc gcctgcagcc
      241 ggtcgaagcc ttgcgcacgg ccctctgata taaaacgcgc ctgatgcgcg gcgcccctgt
      301 cctggtcctc ctcgcggcgc tcgcctggtc cgctcccgcc gccgcagcgc cggccgcgga
      361 cgatcctacg cggccgcttc tctgggcgac cgatgccgaa ggcggggccc cctacacctt
      421 caaggatccg aaagacccga accggaacat cggcttcgag gtcgagatca tcgcggagat
      481 cgagaaggag atcggccagc cgatcgtggc gcaccaatac gccttctcga gcctcatcct
      541 cgggctgcag cgcggggact tcgacttcgc catgaacggg ctcgaagtca cccccgatcg
      601 ggccgagaag gtcctgttca gcgtgccgta ctacgtcttc cgcgagcagc tcgtcgtgcg
      661 caagggggag gcgcgcttcc gcgacctcga agggtgcggg acgatcgact gcgtcgtcgg
      721 taccctcgag gacaccgccg ccgaccgtct gctgcacgcg cgggggatcc gcgcgacgat
      781 gtacgacggc gtggtcgagc cgtaccggga cctggtcctc ggccggctcg acgccgtcct
      841 gctcgacctg ccgatcgcca tctactacgc gaagccgaat ccggacctgg agttcgccgg
      901 cgaggcgatg gcccgcggca cctacgccat catgttccag aagggcaacg aggcgctcaa
      961 gcgccgcatc gacgcggcgc tcgagacgat cctcgaggac gggcggctgc gggccatcct
     1021 ggagcgctgg aacctctggg acgaggagca ggcggcgctc gcccaggtcg gaagcggcga
     1081 catcctctcc gcctcgcgtg atgcctggac gttcgatcgc tacttccctc tgctgctgcg
     1141 cggcgcggtg ctgaccgtgt tcctggcgtt cgtgtccttc ggcctcgcca tcctgctcgg
     1201 gctgccgatc gcctgcatgc gcctctacgg accggcgccg atgcgctggg tcgccatcgg
     1261 ctacgtcgag ttcttccgcg gcatcccggt cctgctgctg ctctacgtgc tgtattacgg
     1321 cctcgccgat ctctccacgg cgagcggcct tgggagcgcc ttccggctcg acccgatcca
     1381 tgcggcgatc ctcggcctcg ggctgaacta cgccgcgtac gaagcggaga tctaccgggc
     1441 cggcatctcc gccgtgccct acgggcagtg ggaggcggcg gcctccctgg ggatgaccgg
     1501 ctggctcgcc ttccggcgga tcatcctgcc gcaggcggtc cgcaccatcc tgccgccgat
     1561 gaccggcgac ttcgtcgcgc tgttcaagga caccagcatc gtctcgacga tcgccgtcgt
     1621 cgagctctcc aagcagtacc agatcctctc caagtcgagc atgaaatacc tcgagatcgg
     1681 gcttttgacc gccgtcctct atctcgtgat gtcggtaccg ctcgggttcc tgtcgcgctg
     1741 gctggagcgg cgctgggggc gcgggatcct gtgaccgcca atcccggcat cgacctgacg
     1801 ctcgaagggc tgcggaaggt gtacgacggc cacaccatcc tcgacgacgt gaccctctcc
     1861 atcgcggcgg gcaccaccgt cgccctcatc ggtccgagcg gcggcggcaa gtcgaccctg
     1921 cttcgctgcc tcaatgggct cacgccgttc gagggcggaa gcgtccgcgt gggcgagcac
     1981 cgcctcggcc cgggcccggt cgaggggccg aaccgcgccg tggcgcaaca gatccgccgc
     2041 ctcctcggga tgatcttcca ggacttccag ctcttcccgc acctgaccgc catcgagaac
     2101 ctcatcgagg cgccggtgcg cgtcctcggg tcgccggccg aggcagcgcg ggcggaggcg
     2161 gatcgcctcc tcgaccgggt cggcctgtcg caccgcaagg acgcctaccc gaacatgctg
     2221 tcaggcgggc agaagcagcg ggtcgccatc gcccgcgcgc tggccatgca cccgcgtggc
     2281 ctcctgtgcg acgagatcac cagcgcgctc gacccggagc tcaaggggga ggtgctggcg
     2341 gtggtggagg acctccgccg cgacggcatc accctggtga tggtgacgca cgagatcggc
     2401 ttcgcgcggc gcgccgccga ccgggtgctg gtcctcgacc acggcgccat cctcgaggac
     2461 ggccccccac gcgaagtgct ggagcgcccg aagacggcgc gcacccgtca gttcctgtcg
     2521 ctgctgatgg gctgagcccc cgcggcagca gaccgcatgt ccggcgctac tgaccgagct
     2581 gggagagcgc gtagcgcatc gggccgctgg agatcttgat cagcccgtcg aacgggccgt
     2641 ccagctcgag agtcaggaag accgcagccg cgaccgagat cgcggcgacg aagagcacgg
     2701 tgatgaccga ggcgttcggg ggcgcgtaga ggccaaagct cacgaatgtc gcggtcaacc
     2761 agaggatcac cacggccaga aacaggagcg gcaccgaccc ttcctggctg cccagcgcgc
     2821 gccagcgcgc cttcatgaca tcctcggtca gcctgagcgc ctcggatttg aaccaccgtt
     2881 gcgcgtcggt ggcgggcgcg agctggagga cctggttctc gatctcctcg accgccggcg
     2941 ttcccaccgc ttgcgtcagc cccgcgccgg ccgatccgtc ctcgggccag gtcgcctcga
     3001 cccgaaaggc cacggcctga cggatgagat cccgggtggc tctggtctcc ggaccgtagc
     3061 gcgcgagaag ccggtcgagc gtgaggatat tggtcgcgtt ggtccgcatc gacgcgtcct
     3121 ggctgtcgaa cgagctgcgc gcagaggccg tcaccaggcc gagcagcaac gcggtcatcg
     3181 tcgcgaccag ccccatcgcg agccgcatga catcctgcga ctctttgctc aggtgtcccg
     3241 acgggaggat tgcgcggaca taggttccga ggagcgcccc tccgaaagcg cacgcgaagg
     3301 tgatcgaggc ggcagcgagg gcactcaaag ccggctcacg cacggattgt aagagcgcca
     3361 cgcgatcctg aacaagcccc cgtgtgtcag accgcctggc caatccattt gaattgtggc
     3421 aatcgaggtg tcgacattga cggcgcgagg tgttccggga taggatgtgc tcctccgaac
     3481 agacgattcg actccctccc atcgccttgt gcggcattcc atcaataagg gggttctcca
     3541 tgaaaatcat gatccgaacc ggactcgcat tcgtcctcgc cctggcgtgc tgtctgccgg
     3601 cgatggccgc cgcggcccct gacaaggccg agaaggtcgc atcggggatg ctgaagttcg
     3661 atgacgtcct cactgagggg acgaccagaa ttagcggagt cctcgccacc atgaactcgc
     3721 tgagcacggc gaccggctcc gacctggtca gcaagtacaa gaccttcacg aagcaggtga
     3781 acgagttgga tgcgaccgcc aagaaggcca aggcccgctc cgaggatgcc acgtcgcagc
     3841 gcgaggagta cctcgagcag tggcaggaat cacagggtca gatccagaac gaggagctca
     3901 aggcggcctc ggcagcccgg cgcgcggagc tgatgccgaa gatcgagggg atcaaggaag
     3961 ccctgaccgc cgccagccag ggcttcgcgc ccttcatgca ggatctcaag gacctgacga
     4021 tcttcctggg gaatgacctg agcgagaagg gggtcgccac ggcgagcgag ctgatggcga
     4081 agtgcaccga gggtggcgcc aaagtgaacg ccgacatcga gcgcggatcc gccggcgtca
     4141 aggatctcgc cgccagcatc agccccacct cggcggccaa gaagtagacc ccgctcgaag
     4201 tcgccgaagc tttccggcta ggagaccacg gcgtcgcgca gcaggcgcgc cgccagctcc
     4261 ttgaccagct tcgccgccac cgccgcggtc gtccccaccg ggtcgcgcag cggattcatc
     4321 tcgacgatgt cggccccaat gatgcggcca cgggtcccct cgaggacgct caggatgtcg
     4381 cgcacgctca gcccccccgg ctcgtggtgc gacacgccgg gggcataggc cgggtcgagc
     4441 gcgtccatgt cgaccgagac gtaaaccggc tgcgcgaatt cgagcctcag gtccggccgg
     4501 aattcgcgca tcgtgatcga ctcgacgccg aaccgggcga tctgctgacg caggtgggca
     4561 ttggtggagc ggacgccgac ctgcaccagc cgcttcacca ggcccttctc caggacgcgg
     4621 gcgaacgggc tcgcgtgcga gtgcgggttg ccgtcgaagt cgtcgtagag gtccgggtgg
     4681 gcgtcgaact gcaggaccgc caggtccgga tgccgggcgg cgtgcgcctg caggatcgga
     4741 aaggtgatcg agtggtctcc tcccagcgcc aggacgcgcg ctccgcgggc gaggaggtcg
     4801 gccacgcccg cctcgatcgc ctgcatcgcg gccgccccct gcgggagcga caggtcgccc
     4861 gtgtcgacga agcgctcgga gccgatgatg accgtcccgt cctcggagca gtcgctcgac
     4921 gagccggaga aaagcgtctc gcggatccgt cccggcgcct gggccggccc gcgcaggagg
     4981 gacgagttct cgtcgagcgg aaccccgacg agcgccacgc tgcctgcgcc aggagaggtc
     5041 gcctgccggc gggtcatcgc cccggtccct tcgctgcgcc cgctgcgatc gtctggccgg
     5101 cgcgcgccga cgcgacgacc ccggccccct cgcggaggac caggaaccgg tccgcctcct
     5161 gggggccgat cgtctcccgc gtcttcgcct tgttcggcca gcggatctcg atcgagtcga
     5221 cgcgggtcgc cgtccccagg ccgaagtgca gccgcatgtc gttctgcgac atgtacgagg
     5281 aggcgctccg cacttcgcgc acctgccggc gctccccggc ggtgagcgtg acgaaggcgc
     5341 cgagggcgtc cttgttcgac cgggtcccga cgagcttgag ggagatccag tggccggcgg
     5401 ccgcgccgcc gtcgttg
//
LOCUS       DASRIQ010000261         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1414885, whole genome shotgun sequence.
ACCESSION   DASRIQ010000261 DASRIQ010000000
VERSION     DASRIQ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414885"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFD06_06455"
     CDS             <1..707
                     /locus_tag="VFD06_06455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN03213.1"
                     /translation="ELDAVMRASLKKRYLYTSLEKANRQLRDANEKLTKLSVTDDRTN
                     LLNDRYLKRRLAEEFKRARRYGAPLTVMLLDLDHFKQVNDKYGHDCGDQVLAEFAKIL
                     VDNAREIDIVGRYGGEEFLIILPNTDGIRAAVVAERVRKAAEEHVYRYREFLVRLTVS
                     IGLAAVPANASVHTEADLLRAADEALYRAKQTSRNKVILDRASIPGDILEGDLSTIFK
                     ASYEDGPAIPPPGDDF"
     gene            797..3142
                     /locus_tag="VFD06_06460"
     CDS             797..3142
                     /locus_tag="VFD06_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZN03214.1"
                     /translation="MAARSTRRTGKKRAGPSRRALLWGVPVAGLALVAGALTIAFIDV
                     NWRFSRLASPPPIRVLSTSFHLREGMRLSREDLVARLQRLGYRQVEGQPSTPGEYSLR
                     FRSIEISRNRFDGPDGPVEPLLVRVRLGSSRVGNVEDAETGRDVIDFRLEPEPLGFLS
                     GDAHEERRRTPIADVPAVLKRAVIAVEDRRFLRHPGFDPLGILRAFFANLREGEVVQG
                     GSTITQQLAKNLYHAGDRRTVPRKIWETLAAISLEMARTKDAILERYLNEVYLAQRGP
                     MAIVGVGAAAQHYFGKEVRYLDLPEAALLAGLIQSPGHYHPYRHPDAAHERRDLVLGL
                     MRDAGFITEDERQAAAAAPLRLRPEPSHDPRQAPYFVDYIAEELRRVGIDDDSSSGLT
                     VFTTLDPLLQARAERALESRLAKDEKNWKQLRPLPGGSLQGALVALRPADGAIVAMVG
                     GRDYGSSQFNRVAQAHRQPGSLFKPFVYLAGFQRAQEEGEASFTPATVLDDSPLEEVV
                     AGKLWAPHNFDEEFRGPVTVRQALTHSLNIPTIRAAEQIGLREVIRAARRCGIESPLE
                     PVPSIALGTFEVTPLEIAAAFSTFANAGERQATRAIEAVVDGRGKRRELPAPERRRAT
                     TPQAAYLTIDLMRDVVRQGTAASLGAWGFEGDLAGKTGTTDEGRDAWFVGFNRDLLAL
                     AWVGFDNNRPLRLEGSALALPIWADFAAGAGLDPQSRFEEPEGLVREQVDPTTGLLAG
                     WRCPDSLDELFIEGSAPVDPCEHDKPFTSWAKRLFHWLKRE"
     gene            complement(3166..>3898)
                     /locus_tag="VFD06_06465"
     CDS             complement(3166..>3898)
                     /locus_tag="VFD06_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03215.1"
                     /translation="PPEFRKIEVLSSGVTLQEVPAAAPGQGQEGKPASGDDAAARHRP
                     RPTSRRGFDAGARSITWQVVDPNEDDLTYDVQYRALDEKAWKTIRTRIDEDFVTFDGS
                     ALPDGTYLVRVIASDALSNPSGLALTAEKISAPFDVDNTPPRIDRLKAQIEHGTLKVS
                     FEANDGFSVVREASYSVDAGEWMPARPEDGLSDSGAETFAIEIPTPPAGECSIVVRAV
                     DGAGNVGAGRTVVEVPEGRATASSH"
BASE COUNT          567 a         1407 c         1372 g          552 t
ORIGIN      
        1 acgagctgga tgcggtgatg cgcgcctcgc tcaagaagcg ctacctgtac acctcgctcg
       61 agaaggcgaa ccggcagctg cgcgacgcca acgagaagct caccaagctg tcggtcaccg
      121 acgaccgcac caacctgctc aacgatcgct acctgaagcg gcggctggcg gaggagttca
      181 agcgggcgcg gcgctacggc gcgccgctca ccgtcatgct cctcgacctc gaccacttca
      241 agcaggtgaa cgacaagtac gggcacgact gcggcgacca ggtgctcgcc gagttcgcga
      301 agatcctcgt ggacaacgcg cgagagattg acatcgtcgg ccgctacggc ggcgaggagt
      361 tcctcatcat cctgccgaac accgacggca tccgcgccgc ggtcgtcgcc gagcgggtgc
      421 gcaaggcggc cgaggagcac gtctatcgct accgcgagtt cctcgtccgg ttgacggtga
      481 gcatcggcct ggccgcggtg ccggccaacg cctcggtgca caccgaggcg gacctgctgc
      541 gcgccgcgga cgaggcgctc taccgggcga agcagacgtc ccgcaacaag gtcatcctcg
      601 atcgcgcctc gatcccggga gacatcctcg aaggggacct ctcgaccatc ttcaaggcct
      661 cctacgagga cggccccgcg attcccccac cgggcgacga tttctgacct cgcgcccggg
      721 gccgcccgcg ccgggcggcc ggccctcgcg gtgatagact cctgaggcgg ttcgcgggag
      781 ccgcgtcgga gcctcgatgg ccgctcgcag cactcgcagg accgggaaga aacgcgccgg
      841 tccctcccgc cgcgctctcc tctggggcgt gccggtcgcc gggctggccc tcgtcgccgg
      901 cgccctgacc atcgccttca tcgacgtcaa ctggcgcttt tcgcgcctgg cctccccgcc
      961 ccccatccgc gtgctttcga cgtcgttcca tctgcgcgaa gggatgcgcc tgtcgcgcga
     1021 ggatctggtc gcgcgcctgc agcgcctcgg ctaccgtcag gtcgaaggcc agccgtccac
     1081 cccgggcgag tactcgctgc gcttccgatc catcgaaatc tcccgcaacc gcttcgacgg
     1141 gccggacggg ccggtcgagc cgctgctcgt gcgggtgcgg ctgggctcgt cgcgcgtcgg
     1201 caacgtggag gacgcggaga ccggccgcga cgtgatcgat ttccgcctcg agccggagcc
     1261 gctcgggttc ctgtcggggg acgcgcacga ggagcgccgc cgcaccccga tcgcggacgt
     1321 ccccgcggtc ctgaagcgcg cggtgatcgc cgtcgaggat cggcgcttcc tgcgccaccc
     1381 cggattcgat ccgctcggca tcctgcgcgc gttcttcgcc aacctgcgcg agggggaggt
     1441 ggtgcagggg gggagcacga tcacccagca gctcgccaag aatctctacc acgcggggga
     1501 ccggcgcacc gtcccccgca agatctggga aacgctggcg gcgatcagcc tcgagatggc
     1561 ccgcaccaag gacgcgatcc tcgagcggta cctcaacgag gtctatctcg cgcagcgcgg
     1621 gccgatggcg atcgtcggcg tcggcgccgc cgcgcagcac tacttcggca aggaggtgcg
     1681 ctacctcgac ctgccggaag cggcgctcct cgcggggctc atccagtccc cgggccatta
     1741 ccatccgtac cggcacccgg acgcggcgca cgagcgccgc gacctggtgc tcggcctgat
     1801 gcgcgacgcc ggcttcatca ccgaggacga gcgccaagcg gccgccgccg cgccgctcag
     1861 gctccggccg gagccgtcgc acgacccgcg gcaggcgccg tacttcgtgg attacatcgc
     1921 cgaggagctg cgccgcgtcg gcatcgatga cgacagctcc tccggcctga ccgtcttcac
     1981 gacgctcgat ccgctgctgc aggcgcgggc cgagcgggcg ctcgagagcc ggcttgcgaa
     2041 ggacgagaag aactggaagc agctgcggcc gctcccgggc gggagcctgc agggggcgct
     2101 cgtggcgctg cgacccgcgg acggcgcgat cgtcgcgatg gtcggcggcc gcgactacgg
     2161 gagctcgcag ttcaaccgcg tcgcgcaggc ccaccgccag cccggctccc tcttcaagcc
     2221 gttcgtctac ctcgccgggt tccagcgggc gcaggaggag ggggaggcga gcttcacgcc
     2281 ggcgacggtc ctcgacgact cacccctgga agaggtggtc gcggggaagc tctgggcgcc
     2341 gcacaacttc gatgaggagt tccggggacc ggtgacggtg cgccaggcgc tgacgcacag
     2401 cctgaacatc ccgaccatcc gggcggcgga gcagatcggc ctgcgcgagg tgatccgggc
     2461 ggcgcggcgc tgcggcatcg agagcccgct cgagccggtc ccgtcgatcg cgctcgggac
     2521 cttcgaggtg acgccgctcg agatcgccgc cgccttctcc acgttcgcga acgcggggga
     2581 gcggcaggcg acgcgcgcca tcgaggcggt ggtggacggc cgcggcaagc ggcgcgaact
     2641 gccggcgccg gagcgccggc gcgcgaccac cccccaggcg gcctacctga cgatcgacct
     2701 gatgcgcgac gtggtgcgcc aggggaccgc cgccagcctc ggcgcgtggg gcttcgaggg
     2761 ggatctcgcc ggcaagaccg gaacgaccga tgaagggcgt gatgcgtggt tcgtcggctt
     2821 caaccgggac ctgctggcgc tcgcctgggt ggggttcgac aacaaccggc cgctgaggct
     2881 cgagggctcc gccctggcgc tgccgatctg ggccgatttc gcagccggcg ccgggctcga
     2941 tccccagtcc cgcttcgaag agccggaggg gctggtgcgg gagcaggtcg atccgacgac
     3001 cgggctgctc gccggctggc gctgccccga ctcgctggac gagctgttca tcgaggggag
     3061 cgcgccggtc gacccgtgcg agcacgacaa gccgttcacc tcctgggcga agcgtctctt
     3121 ccactggctc aagagggaat agccgcttgg cctcacgaaa ccacctcagt gggaggaggc
     3181 cgtagcgcgc ccttcgggaa cctcgacgac ggtacggccg gcgccgacgt tgccggcacc
     3241 gtccacagcg cgcacgacga tgctgcactc ccccgccggc ggcgtcggga tctcgatggc
     3301 aaaggtctcc gcgcccgagt cgctcaggcc gtcctcagga cgggccggca tccactcccc
     3361 ggcgtcgacc gagtaggacg cctcccgcac cacgctgaag ccgtcgttgg cctcgaagga
     3421 caccttgagg gtgccgtgct cgatctgcgc cttgaggcgg tcgatgcgcg gcggcgtgtt
     3481 gtccacgtcg aacggcgccg agatcttctc ggcggtcagc gccaggccgc tcgggttcga
     3541 caacgcgtcg ctggcgatga cgcgcacgag gtaggtgccg tccggcagcg ccgacccgtc
     3601 gaaggtgacg aagtcctcgt cgatgcgggt gcggatcgtc ttccacgcct tttcatcgag
     3661 cgcccggtac tgcacgtcgt aggtgagatc gtcctcgttg ggatcgacca cctgccaggt
     3721 gatgctgcgc gctcccgcgt cgaagccgcg gcgcgaggtc gggcgcggcc ggtgccgcgc
     3781 cgccgcgtcg tcgccggacg ccggcttccc ctcctgcccc tgtccggggg ccgcggcggg
     3841 gacctcctgt agcgtcacgc cggacgacag cacctcgatc ttgcggaact cggggggc
//
LOCUS       DASRIQ010000262         7781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2438336, whole genome shotgun sequence.
ACCESSION   DASRIQ010000262 DASRIQ010000000
VERSION     DASRIQ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2438336"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFD06_06470"
     CDS             <1..345
                     /locus_tag="VFD06_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03216.1"
                     /translation="LGVWPDYVVVDALTLPGLPIPQEGITGGDRTCASIAAASIVAKV
                     HRDALMRRLHDTWPAYGFALHKGYGTATHLDALRKHGASPVHRLTFRGVRMPDEALLL
                     PFARPDDAAEES"
     gene            349..3438
                     /locus_tag="VFD06_06475"
     CDS             349..3438
                     /locus_tag="VFD06_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03217.1"
                     /translation="MPALDRAKTLQAAEKLVRAGKLLEAAKEYQKLADDNPRDMNLVN
                     KLGDLLLRGGQNQAALKQFMRIADFFAKDGFHLKAIAMYKKISKIDPSNLECQQRLAL
                     LYHEQGLATEAKAQYQQVADAFVKSGRTPGAAEALRRLLEIDPDNAKVRMSLADLLGR
                     NNQNDDAAVQYAHLARVAAARGAVDEAIAVVRKAIKLSPKNAELPGILLAVVTRVEQA
                     PAELVQAAEEIARHAAKSGRAVLLMAEVLKRAGRVDEADETIRRLDGEDFEDELDLES
                     LTLVGRFHADRGRVADGYRWIDRSVEQLVAGGKLDEAASVVDVFLHAHDSHPEALQRR
                     AAIAAKAGDAEAEARALQRLASNLMDQGHSDRARPAVDRLATLRPGDPATVELVERLQ
                     GGKAKPAVAPTRAAPPPAPEPPPVPAPRPAPAAPTQAASEDEEASEIFNLDEEDAEPP
                     AIEAVEEESVGQDEELAIEIEADDSSEEDAYGGKSRIQEIQDADPSGQPIDEEFISEH
                     LTEAEVFVKYGLLDKAREQLKAILKKYPQNDTAHVRLKDLAVSEGNKDAAVRACLALA
                     DIRRSQGKDDEARGLVNEAVRIDPDHPELQRLAIAADRPAGPRIVPAAPPPVPPAAAK
                     KGKPAAPAPPPPPAPEFEDDLEIDLDLPEETAPAPAPAPPPAAAKATKPKPAAAPDFA
                     TTTAKTVLPIVEEDAGEDATDDLEMLRDALAEPDSEKLGEVDFYIEQGLVDEARQILF
                     QLNKRFPGSDAISERMERLESPRAHASAASAPAAGEDEGVDFEVEQALTGKQRAVEPA
                     HKPAPPAAKARAATPAPPEPKAAKKPHAAPVAPPKAAPVRPVFKMETPVADETGDFFD
                     LAGELDRSMEDEQAKTDAAAKEALDSQAHTFEDIFAAFRKGVEQQVDSDDFDTHYNLG
                     IAYKEMGLVDEAIGEFQFAARDPNRALECCGILGLCFRDKGMPDLALKWYKRGLDMPG
                     LDEHQSVGLRYDMAEVHREKGEVDEALRIYMEVFGVDSTYREVGSRIKELKAQSGKR"
     gene            4000..5493
                     /locus_tag="VFD06_06480"
     CDS             4000..5493
                     /locus_tag="VFD06_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN03218.1"
                     /translation="MTSQPFRNLIDGEWVPARSGRTFEDLSPADTRTLVGEFPDSSAE
                     DVAAAVVAAARAFRSWRLVPAPKRGEIVFRMGDILARRKEDLARAMAQEMGKPLREAR
                     GDVQEGIDVCYHIGGEGRRLHGFTTPSELPDKTCFTMRQPVGVCGLIAPWNFPLAIPS
                     WKLIPALVCGNAVVLKPASAAPLLAWHLAAIAQEAGAPRGVVNFVTGAGGAVGEAIVR
                     HPGTALVSFTGSSDTGRRVAIACAEGFKRVSLEMGGKNALIVCDDADLDLAVDGALWG
                     AFGTAGQRCTATSRIVVDQRVHDEFVRRLVERAGLLRVGDPLDDSNDVGPLVSDGQRR
                     TVEEYVAIGRKEGARLACGGVRLEDGGRERGFFFAPTVFTGVEPGMRIAREEIFGPVV
                     SVLRADGFEEALRIANDTEYGLSSAIYTRDVDRAFRAARDLEAGITYVNSSTIGAETH
                     LPFGGTKRTGNGHREGSAATAIEIFTEWKTVYVDHSGRLQKAQIDNN"
     gene            5555..6670
                     /gene="ald"
                     /locus_tag="VFD06_06485"
     CDS             5555..6670
                     /gene="ald"
                     /locus_tag="VFD06_06485"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZN03219.1"
                     /translation="MQIGVPKEIKDNEYRVSLVPAGVRALVEHGHQVLVQDGAGLGSG
                     IENIAYEQAGAVVVADAPAVFRRADLVVKVKEPLSSETKLLRKGQILFTYLHLAPAPE
                     LTRALIESGCTAIAYETITDADGGLPLLTPMSEVAGRMAIQVGAYFLTRPMGGRGVLL
                     AGVPGVPHGDVVILGGGVVGINAAFMALGLGAKVTVLDRSLPRLRYLDEIFRGQVTTV
                     YSTTAYVHEAVRRADLLVGAVLVPGASAPRLVTKAMLGHMKKGSVIVDVAVDQGGCIE
                     TTHPTTHSDPTYVVDGVVHYCVANMPGAVPRTSTFALTNATLPYILKIAGKGLADALR
                     ADTTLRPGVNVHAGKVTCEAVASSQGLPYTPLESTLS"
     gene            complement(6675..>7781)
                     /locus_tag="VFD06_06490"
     CDS             complement(6675..>7781)
                     /locus_tag="VFD06_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZN03220.1"
                     /translation="YQPNNYYRKYYGITTLREAMEESRNIVAVKLLNEVGYGRTIDTA
                     RRMGISSALRPYPSMALGTMEVSLWDLTTAFSVFANQGVRVEPHFIRQVADREGRVRE
                     AAKPRVVEAIRADVAYLMTYLLEGVTEAGTGAAAASALDRVVAGKTGTTDDLADAWFV
                     GFSPSLVAGAWVGFDQKKTLGEGETGARAALPIWIDFMRAAHEGKPVEKFNKPANVVY
                     TPIDRRLGRLATSDSHCPVPFLEAFVEGSEPSEPCSEVEHFRATLPYYLQKFEISRDF
                     ELRIEPDVVAKLVREGSGDLELAPDGKELVLHEEEGKERRLRLDLSRGERREIQNDLA
                     VLQPIDPQDGGAGDGEVPVGIDGRPATIVPIKPD"
BASE COUNT         1259 a         2697 c         2732 g         1093 t
ORIGIN      
        1 ctcggggtgt ggcccgatta cgttgtcgtg gacgccctca ccctgccggg cctgccgatc
       61 ccccaggagg ggatcacggg cggggaccgg acctgcgcgt ccatcgccgc cgcctccatc
      121 gtcgccaagg tgcaccggga cgctctcatg cgccgcctgc acgacacctg gccggcctac
      181 ggcttcgcat tacataaagg ttacgggacc gcgacccacc tggacgccct ccgaaaacat
      241 ggcgcatcac ccgttcaccg ccttacattc cggggggtgc ggatgccgga cgaggcgctc
      301 cttctgccct tcgctcgtcc ggacgacgcc gccgaggaga gctgaggcat gcccgcgctc
      361 gaccgcgcga agacgctgca ggccgccgag aaactggtgc gcgccggcaa gctgctcgaa
      421 gcggccaagg aataccagaa gctcgccgac gacaacccgc gcgacatgaa cctcgtgaac
      481 aagctcgggg atctcctgct ccgcgggggc cagaaccagg ctgccctcaa gcagttcatg
      541 cgcatcgcgg atttcttcgc caaggacggg ttccacctca aggcgatcgc gatgtacaag
      601 aagatctcca agatcgatcc cagcaacctc gagtgccagc agcgcctggc gctgctctac
      661 cacgagcagg ggctcgcgac cgaggcgaag gcgcagtacc agcaggtcgc cgacgccttc
      721 gtgaagtccg gcaggacgcc cggcgcggcg gaggcgctgc gccgcctgct cgagatcgat
      781 cccgacaacg ccaaggtgcg catgtcgctc gccgatctcc tcgggcgcaa caatcagaac
      841 gacgacgcgg cggtgcagta cgcccacctg gcgcgcgtcg ccgccgcccg cggcgcggtg
      901 gacgaggcga tcgcggtggt gcgcaaggcg atcaagctga gcccgaagaa cgccgagctg
      961 cccggtatct tgttggcggt ggtgacgcgg gtcgagcagg ccccggccga gctggtgcaa
     1021 gcggcggagg agatcgcccg gcacgcggcg aagagcggcc gcgccgtgct gctcatggcc
     1081 gaggtgctga agcgcgccgg acgggtcgac gaggcggacg agacgatccg ccggctcgac
     1141 ggcgaggact tcgaggacga gctcgatctc gagtcgctca cgctggtcgg gcggttccac
     1201 gccgaccgcg gccgggtcgc cgacggctat cgctggatcg accgttccgt cgagcagctg
     1261 gtggccggcg gaaagctcga cgaggcggcc tccgtggtgg acgtcttcct gcacgcgcac
     1321 gactcgcacc cggaggcgct ccagcgtcgc gccgccatcg ccgccaaggc gggggacgcc
     1381 gaggcggagg ctcgggcgct gcagcgcctc gcgtcgaacc tcatggacca gggtcactcg
     1441 gaccgcgcgc gccccgccgt cgaccggctc gcgacgctgc gccccggcga tccggccacg
     1501 gtggagctgg tggagcgctt gcaggggggc aaggcgaagc cggcggtcgc gccgacccgt
     1561 gccgcgcccc cccccgcgcc cgaacccccc cccgttcctg cgccacgccc ggcccccgcg
     1621 gcgcccaccc aggccgcgtc cgaggacgag gaggcgagcg agatcttcaa cctggacgag
     1681 gaagacgccg agccgccggc catcgaggcg gtggaggagg agagcgtcgg ccaggacgag
     1741 gagctggcga tcgagatcga agccgacgac tcgtccgagg aggacgcgta cggcggcaag
     1801 agcaggatcc aggagatcca ggacgcggac cccagcggac agccgatcga cgaggaattc
     1861 atcagcgagc acctgaccga ggcggaggtg ttcgtgaagt acggtctgct cgacaaggcg
     1921 cgcgagcagc tgaaggcgat cctcaagaag tacccgcaga acgacacggc gcacgtgcgg
     1981 ctcaaagatt tggcggtgag cgaagggaac aaggacgccg cggtgcgcgc ctgcctcgcg
     2041 ctcgccgaca tccgacgttc ccaggggaag gacgacgagg cgcgcgggct ggtgaacgag
     2101 gcggtgcgca tcgatcccga ccatcccgag ctgcagcgcc tggcgatcgc ggcggatcgg
     2161 ccggccgggc cgcgcatcgt cccggccgcc ccgcctccgg ttccgcccgc cgcggcgaag
     2221 aaggggaagc cggccgcgcc cgctccgccc ccgccgccgg ccccggaatt cgaggatgac
     2281 ctggagatcg atctcgatct tccggaggag actgctccgg ctcccgcgcc tgccccccct
     2341 ccggccgcgg cgaaggcgac caagccgaag cccgcggcgg cgcccgactt cgcgaccacc
     2401 accgcgaaga cggtgctgcc gatcgtcgag gaagacgccg gggaggatgc gaccgacgac
     2461 ctggagatgc tccgcgacgc gctggcggag ccggattcgg agaagctcgg agaggtcgac
     2521 ttctacatcg agcaggggct ggtggacgag gcgcggcaga tcctcttcca gctcaacaag
     2581 cggttccccg gaagcgacgc catctccgag cggatggaac ggctcgagag tccccgggcg
     2641 cacgcgtcgg ccgcctcggc gccggccgcg ggagaggacg agggcgtcga cttcgaggtg
     2701 gagcaggcgc tgaccgggaa acagcgcgct gtggagccgg cccacaagcc cgcccctccg
     2761 gcggcgaaag cccgggcggc cactccggcc ccgccagagc cgaaggccgc gaagaagcct
     2821 cacgccgcgc cggtcgcgcc gccgaaggcg gctccggtgc ggccggtctt caaaatggag
     2881 accccggtgg cggacgaaac cggggacttc ttcgacctcg ccggagagct cgaccgatcg
     2941 atggaagacg agcaggcgaa gaccgacgcg gccgccaagg aagccctgga cagccaggcc
     3001 cacactttcg aggacatctt cgccgccttc cgcaaggggg tggagcagca ggtcgactcg
     3061 gatgacttcg acacgcacta caacctcggc atcgcctaca aggagatggg gctcgtcgac
     3121 gaagcgatcg gggagttcca gttcgcggcg cgcgatccga accgcgccct cgagtgctgc
     3181 ggcatcctcg gcctctgctt ccgcgacaag gggatgcccg acctggcgct caagtggtac
     3241 aagcgcgggc tcgacatgcc ggggctcgac gagcatcagt cggtcggcct gcgctacgac
     3301 atggccgagg tgcaccgcga gaagggggag gtcgacgagg cgctccggat ttacatggag
     3361 gtcttcggcg tcgattccac ctaccgcgag gtcggcagcc gcatcaagga gttgaaggcg
     3421 caatcaggga agcgctgacc gggggcggac agtccggaca gagctgcata ccagttccgc
     3481 gccaattcgt cgtcctgaag tgtttccctg actccgtccc tgcacttccc gcaggtgctc
     3541 caggatcgtt taactcgttg attcgacgtt agaaacatgt cgcggaacga ccgtttctac
     3601 ctctcagcct tccgcgggca catggattgc ttctaaggcg gcggaaccga cgcagcgaat
     3661 caagcgttca ctgcatcggg cactgaagag gttcgttgga tgcaacggtc ggtccgtcac
     3721 gcatgccgga tgagcagtga ccctcgaaag accccctgcc atcctagatg cacccaccta
     3781 caagagcgag agcaacagat tcggcgaata cggcgatgcc ggtagcgtga cggaccgacc
     3841 gaacctctcc tcgaaaacga ttcgcaggaa accctgactc ctcgaaggcc tttcgacacg
     3901 gtgccttgac cgtgcccgaa cgggtctgtt agcatcacgc cccaaccaat tggcgacaaa
     3961 ggacttcgct cagccgtcgc gggtggaggc aggtcgggtg tgacgtcgca gccattccgc
     4021 aacctcatcg acggagagtg ggttcccgcc cgcagcgggc gcaccttcga agatctcagt
     4081 cccgccgaca cgcgcaccct cgtcggcgag tttcccgact cctcggccga ggacgtcgcc
     4141 gccgcggtag tggccgccgc gcgcgccttc cgatcgtggc gcctcgtccc ggccccgaag
     4201 cgcggcgaga tcgtcttccg gatgggagac atcctggcgc ggcgcaagga ggatctggcg
     4261 cgcgccatgg cgcaggagat gggaaagccg ctgcgcgagg cgcgcggcga cgtccaggaa
     4321 gggatcgacg tctgctatca catcggcggc gaggggcggc gtctgcacgg cttcaccacc
     4381 ccctcggagc ttccggacaa gacctgcttc accatgcgcc agcccgtggg ggtgtgcggg
     4441 ctcatcgctc cctggaactt cccgctggcg atcccctcgt ggaagctgat ccccgccctc
     4501 gtgtgcggca acgccgtcgt cctcaagcct gcgagcgccg cgccgctcct cgcctggcac
     4561 ctggcggcga ttgcgcagga ggccggcgcg ccgcgcggcg tggtcaactt cgtcaccggt
     4621 gccggagggg cggtcgggga ggcgatcgtc cgccatcccg ggaccgcgct cgtgtctttc
     4681 accggatcct ccgacaccgg gcggcgcgtc gccatcgcgt gcgccgaggg attcaagcgg
     4741 gtctccctcg agatgggagg gaagaacgcg ctcatcgtct gcgacgacgc cgacctcgat
     4801 ctcgccgtgg acggggcgct gtggggggcg ttcggcaccg ccgggcagcg ctgcaccgcc
     4861 accagccgga tcgtcgtcga tcagcgcgtg cacgacgagt tcgtccggcg cctggtggag
     4921 cgcgccggcc tccttcgggt gggggatccc ctcgacgatt cgaacgacgt cgggccgctc
     4981 gtcagcgacg gtcagcgccg caccgtcgag gagtacgtgg cgatcggccg gaaggagggg
     5041 gcgcgcctgg cgtgcggcgg cgtccggctc gaagacggcg gacgggagcg cggcttcttc
     5101 ttcgcgccga ccgtcttcac cggggtcgag ccggggatgc gcatcgcgcg cgaggagatc
     5161 ttcggtccgg tggtgtcggt gctgcgggcg gacggcttcg aggaggcact gcggatcgcc
     5221 aacgacacgg agtacggtct gtcgtcggcg atctacacgc gcgacgtgga ccgggcgttc
     5281 cgggcggcgc gcgatctcga ggccggcatc acctacgtga actcctccac catcggggcg
     5341 gagacccacc tgccgttcgg cgggacgaaa cggaccggga acggccaccg cgaggggtcg
     5401 gccgcgaccg ccatcgagat cttcaccgag tggaagacgg tgtacgtcga ccactccggg
     5461 cggctgcaga aggcgcagat cgacaacaac tgatcgaccg gcctccgcgc cgccgtggcg
     5521 gcgcggaggc gcacgggggc gcgcaagagt gagcatgcag atcggggtgc cgaaggagat
     5581 caaggacaac gagtaccgcg tcagcctcgt ccccgcgggg gtgcgggctc tggtcgagca
     5641 cgggcaccag gtcctggtgc aggacggcgc ggggctcggc agcggcatcg agaacatcgc
     5701 ctacgagcag gccggcgccg tcgtcgtggc cgacgcgccg gcggtcttcc ggcgcgccga
     5761 cctcgtcgtc aaggtcaagg agccgctctc ctccgagacg aagctcctgc gcaagggaca
     5821 gatcctgttc acctacctgc acctcgcccc ggctccggag ctgacgcgtg ccctcatcga
     5881 gagcggctgc accgccatcg cctacgagac gatcaccgac gcggacggcg ggctgccgct
     5941 gctgacgccg atgagcgagg tggccggacg gatggcaatc caggtcggcg cgtacttcct
     6001 gacgcggccg atgggaggac gcggcgtgct cctcgccggc gtcccggggg tgccgcacgg
     6061 cgacgtggtg atcctcggcg gcggcgtcgt cgggatcaac gccgcattca tggccctcgg
     6121 gctcggcgcc aaggtgacgg tgctcgatcg atcgctgccg cgtctgcgct atctcgacga
     6181 gatcttccgc gggcaggtga cgacggtcta ctcgaccacc gcctacgtcc acgaggcggt
     6241 gcggcgcgcc gacctcctcg tgggggccgt cctcgtcccg ggggcgtcgg cgccgcgtct
     6301 ggtgacgaag gccatgctgg gacacatgaa gaagggatcg gtgatcgtcg acgtggcggt
     6361 cgaccaggga ggctgcatcg agaccacgca cccgaccacc cactcggatc cgacctacgt
     6421 cgtggacggg gtcgtgcact actgcgtcgc caacatgccg ggggcggtgc cgcgcacctc
     6481 caccttcgcg ctgaccaacg ccacgctccc ctacatcctg aagatcgcgg ggaaggggct
     6541 cgcggacgcg ctgcgcgcgg acaccaccct cagacccggc gtgaacgtgc acgccggcaa
     6601 ggtgacctgc gaggcggtgg cgtcgtccca ggggcttccc tacacgccgc tggagtcgac
     6661 cctctcctag gctcctagtc gggcttgatc gggacgatgg tggcggggcg gccgtcgatt
     6721 ccgaccggca cctcgccgtc gccggccccg ccgtcctgcg ggtcgatcgg ctggagcacc
     6781 gccaggtcgt tctggatctc gcgccgctct ccgcggctca gatcgaggcg caggcgccgc
     6841 tccttcccct cctcctcgtg caggaccagc tccttgccgt cgggggcgag ctcgagatcg
     6901 ccggacccct cgcgcaccag tttcgcgacc acgtccggct cgatgcgcag ctcgaagtcg
     6961 cggctgatct cgaatttctg gaggtagtag gggagggtgg cgcggaagtg ctccacctcg
     7021 gagcacggct cactcggctc gctcccctcg acgaacgcct cgaggaacgg caccgggcag
     7081 tgtgagtcgg acgtcgcgag ccgtccgagg cggcggtcga tcggcgtgta gacgacgttg
     7141 gccggcttgt tgaacttctc caccggcttc ccttcgtgcg cggcgcgcat gaagtcgatc
     7201 cagatcggca gcgccgcccg ggccccggtc tccccctcgc cgagggtctt cttctggtcg
     7261 aagccgaccc aggcgccggc gacgaggctc gggctgaagc cgacgaacca ggcgtccgcc
     7321 aggtcgtcgg tcgtcccggt cttgccggcg acgacccggt cgagcgccga cgccgccgcg
     7381 gcgccggtgc cggcctcggt caccccttcg agcagatagg tcatgaggta ggcgacgtcg
     7441 gcccggatcg cttcgacgac gcgcggcttc gccgcttcgc gcacgcgccc ctcccgatcc
     7501 gccacctggc ggatgaagtg cggctcgacc cgcaccccct ggttggcgaa gaccgagaag
     7561 gcggtggtca ggtcccagag cgacacctcc atcgtgccga gcgccatcga tgggtacggg
     7621 cgcagggcgc tcgagatccc catgcgacgc gccgtgtcga tggtgcgtcc gtagccgacc
     7681 tcgttgagga gcttcaccgc cacgatgttg cgcgattcct ccatcgcctc gcgcagcgtc
     7741 gtgatgccgt agtacttgcg gtagtagttg ttcggctggt a
//
LOCUS       DASRIQ010000263         5333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3775728, whole genome shotgun sequence.
ACCESSION   DASRIQ010000263 DASRIQ010000000
VERSION     DASRIQ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3775728"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFD06_06495"
     CDS             complement(<1..248)
                     /locus_tag="VFD06_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03221.1"
                     /translation="MNNKPYDARPRSRDGSGRAAGHAVGSPLRRVEPSLLILAVLTLG
                     VAACLACSRSKSGAAGTASSASVAGGSGDAPDAQGPGAV"
     gene            complement(265..3816)
                     /gene="mfd"
                     /locus_tag="VFD06_06500"
     CDS             complement(265..3816)
                     /gene="mfd"
                     /locus_tag="VFD06_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZN03222.1"
                     /translation="MNRDVRALLDLPRRDAALAELLRRLDTPDGRLTLAGLSGPAAAV
                     LLPSLLDRAPGGKIVLLVPGERESEPFRHDLEFAASALRERGVRVRLFPSLEADPYQE
                     MTPHLQVACDRVEALRALREPGEAIVVVPVRALIYPLAPPRTLDIATFDLRAGQSLRP
                     DDLSEALLAAGYDRADLVESMAEFSRRGGIIDLFPPDGALPLRVEFWGEEVESLRRFD
                     PATQRSGEAVASARVPPVREYPWDAAALRRLHEALEARRIERRGHAAALRADDLAEKV
                     EALASGRTFPGVEACVRLVEPEPATLFDYAPSALLTAWEPAKVLSELEAVYVEMHAAL
                     DLTEAFDMPMPEDLLVPRHEAQAAIGRARLRLMELAIEEETPPPRVACAPAPTYRGRI
                     QDLAKDLAALPPPTATICLMDSAGRIERLAEVLTQYGQPPAVSTDEALPGMPSIGAGP
                     GIPPGAAPRVLVAPGRLSQGFVLSGARVQVLTEQDVFGAHAEPERKRRKVAAFSPGFK
                     DLKVGDLVVHAEHGIARYGGIARVGENEGQRDFMLLFYEGNDKLYVPVDRLDLVQRYS
                     GVAHHRARLDRLGGITWERTQRKVKKAMRDMAEELLALYAARAAATGHAFPPDTPWQK
                     EFEDAFPFPLTPDQERAVKDTKADMEKDGPMDRLICGDVGFGKTEVAMRAAFKAVMDG
                     RQVAVLCPTTVLTFQHLRTFRERFASWPAQIDMISRFRSAKEQKAILNAAKEGKLDIL
                     IGTHRLLSKDVAFHRLGLLIVDEEQRFGVKHKESVKEMRKDVDVLTLTATPIPRTLQM
                     SLGGIRDMSIIETPPENRLAIQTAVVPFKEGTIASALRHELQRGGQVYFVHNRVESIG
                     SMANFLRRIVPEARVIVGHGQMSEGKLEQTMMAFVKGEADVLLATTIIENGLDIPRVN
                     TLVVNRADRFGLAQLYQLRGRVGRSDRRAYAFLMVPGRKALSDVARRRLKALQEFTEL
                     GSGFRIAAMDLEIRGAGNLLGGEQSGHIAAVGFEMYVSLLERAVRELKGEAPQPETKA
                     QINLNVDIRLPEEYVPDFGDRLVFYKEVASAPDQPTVDRLADRMRDLYGELPPQAQRL
                     LALARLRIEADAVQAKTIDAVRGGVQIRLSESSPIPPETVVRIVNTRRHVRVVPPGLL
                     RLDLPAEASDLERITEVRALLLDLGARDRLAASPARS"
     gene            complement(3813..4334)
                     /gene="rfaE2"
                     /locus_tag="VFD06_06505"
     CDS             complement(3813..4334)
                     /gene="rfaE2"
                     /locus_tag="VFD06_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HZN03223.1"
                     /translation="MGRRKAAAHAAGLRRRGARVVFTNGVFDILHVGHLDLLERARAL
                     GDVLVVGVNSDASVRRLKGNGRPIVPLRERMEMLAGLKPVDVVVAFGEDTPAAIIAAI
                     RPAVLVKGADYRADQIVGADTVRALGGRVVRVRLSHGRSTSRIVSRARTAVRAGQRAR
                     TAMRAAAGRDRRR"
     gene            complement(4388..5194)
                     /locus_tag="VFD06_06510"
     CDS             complement(4388..5194)
                     /locus_tag="VFD06_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HZN03224.1"
                     /translation="MTAPSLSIVIPAYNEAGRIRDSLERLHVWLAVPGRLPGPVEILV
                     VDDGSRDDTAAIVEAFAAGAVARPAGAAAGSALRLLRNGVNRGKGYSLKHGVLLAAGT
                     YVLLSDADFSTPIDELPVLLNPVAASECDIAIGSRGLRGSRVELRQPIWRQTMGRIFN
                     RIVRMLTRLPYRDTQCGFKLMRREAAAPLFRAARVERFAYDVEILMLARKAGLRVREV
                     PVLWRDAPGSKVHAVKDSLAMLRDVLQVLWREHRGRYDRLAPQRDGAPAS"
     gene            complement(5191..>5333)
                     /locus_tag="VFD06_06515"
     CDS             complement(5191..>5333)
                     /locus_tag="VFD06_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase delta/epsilon chain alpha-helix
                     domain-containing protein"
                     /protein_id="HZN03225.1"
                     /translation="EQAKERAEQRLKSAAPETDFERAQQAMRKALLRAEVGKSRHRRE
                     GP"
BASE COUNT          725 a         1982 c         1882 g          744 t
ORIGIN      
        1 accgcccccg gcccctgcgc gtccggcgcg tctccggacc ctcccgccac cgacgcggaa
       61 ctcgcggtcc cggccgcgcc gctcttcgag cgggagcacg ccaggcatgc ggccacacca
      121 agcgtgagca cagcgagaat gagcagcgag ggctcgactc gcctcaaggg cgagccgacc
      181 gcgtgccccg cggccctgcc gctgccgtcc cggcttcgtg ggcgtgcgtc gtagggtttg
      241 ttgttcattc gttctccgcg ctgactagga gcgcgccggc gaggccgcga gcctatcacg
      301 cgctcccagg tcgagcaata acgcccggac ttccgtgatg cgctcgagat ccgacgcctc
      361 cgccggcagg tcgagacgca ggaggcccgg gggaacgacc cgcacgtgcc gccgggtgtt
      421 gacgatccgg accaccgttt cggggggaat ggggctgctt tccgagaggc gaatctgcac
      481 gccgccgcgc accgcgtcga tcgtcttcgc ctgcacggcg tccgcctcga tccggagccg
      541 cgccagcgcc aggaggcgct gcgcctgcgg cggcagctcg ccgtagaggt cgcgcatccg
      601 gtcggcgagg cgatcgaccg tcggctggtc gggggccgac gcaacctctt tatagaagac
      661 cagccggtcg ccgaagtccg ggacgtactc ctccggcagg cggatgtcca cgttgaggtt
      721 gatctgcgcc ttggtctcgg gctggggcgc ctcccccttg agctcccgca ccgcccgctc
      781 caggagcgac acgtacatct cgaaaccgac ggcggcgatg tggccgctct gctccccgcc
      841 gagcaggttt ccggcgccgc ggatctccag gtccatcgcg gcgatgcgga acccggatcc
      901 cagctcggtg aattcctgca acgccttgag ccgccgccgc gccacgtcgc tcagggcctt
      961 ccgccccgga accatcagga acgcgtacgc ccgccggtcg gaccgcccga cgcgcccgcg
     1021 cagctggtac agctgcgcca ggccgaaccg gtcggcgcgg ttgacgacga gggtgttgac
     1081 ccgcgggatg tcgaggccgt tctcgatgat cgtcgtcgcc aggagcacgt ccgcctcccc
     1141 cttcacgaac gccatcatcg tctgctcgag cttcccttcg ctcatctgcc catgcccgac
     1201 gatgacgcgc gcctccggca cgatgcggcg caggaagttc gccatggaac cgatcgattc
     1261 gacccggttg tgcacgaagt acacctgccc gccgcgctgc agctcgtggc ggagggcgga
     1321 ggcgatcgtc ccctccttga acggcacgac cgcggtctgg atggcgagcc ggttctcggg
     1381 gggcgtctcg atgatcgaca tgtcgcggat gccgccgagc gacatctgca gcgtccgcgg
     1441 gatcggcgtc gcggtcagcg tcaggacgtc cacgtccttc cgcatctcct tgaccgattc
     1501 cttgtgcttg acgccgaagc gctgctcctc gtccacgatg agcagtccga gccggtggaa
     1561 cgccacgtcc ttcgacagca gccggtgcgt gccgatgagg atgtcgagct tcccttcctt
     1621 cgcggcattc aggatggcct tctgctcctt tgcgctccgg aagcgcgaga tcatgtcgat
     1681 ctgcgccggc cacgacgcga agcgctcgcg gaaggtccgg aggtgctgga acgtcagcac
     1741 cgtcgtcggg cacaggaccg ccacctgccg gccgtccatc accgccttga aggcggcgcg
     1801 catggcgacc tcggtcttcc cgaagccgac gtcgccgcag atgaggcgat ccatcggccc
     1861 gtccttctcc atgtcggcct tggtgtcctt gaccgcccgc tcctggtcgg gggtgagcgg
     1921 gaaggggaac gcgtcctcga actccttctg ccacggcgtg tcgggcggga aggcgtggcc
     1981 ggtcgcggcg gcgcgcgcgg catagagggc gagcagctcc tccgccatgt cgcgcatcgc
     2041 cttcttcact ttgcgctggg tgcgctccca ggtgatgccg ccgaggcggt cgagccgcgc
     2101 cctgtggtgc gcgacgccgg agtaccgctg cacgaggtcg aggcgatcga ccggcacgta
     2161 cagcttgtcg ttcccctcgt agaacaacag catgaagtcg cgctggcctt cgttctcccc
     2221 gacgcgcgcg atgccgccgt agcgggcgat gccgtgctcg gcgtgcacca cgaggtcgcc
     2281 gaccttgagg tccttgaagc cggggctgaa ggcggcgacc ttgcggcgct tccgttccgg
     2341 ctcggcgtgc gcgccgaaga cgtcctgctc ggtcagcacc tggacgcggg cccccgacag
     2401 gacgaacccc tgcgacagtc gccccggcgc caccagcacc cgcggcgccg cgcccggggg
     2461 gatcccgggt cccgcaccga tcgagggcat gccgggcagc gcttcgtcgg tggagacggc
     2521 ggggggctgg ccgtactgcg tcagcacctc ggcgaggcgc tcgatccggc cggcgctgtc
     2581 catgaggcag atggtggccg tcggtggagg gagcgccgcg agatccttcg ccaggtcctg
     2641 gatgcgcccg cggtaggtcg gggcgggggc gcaggcgacg cgcggcggcg gcgtctcctc
     2701 ctcgatcgcc agctccatca gtcgcaggcg ggcgcggccg atcgccgcct gcgcctcgtg
     2761 gcgcggcacg agcaggtcct ccggcatcgg catgtcgaac gcctcggtga ggtcgagggc
     2821 ggcgtgcatc tcgacgtaca ccgcctcgag ctcggagagg accttcgccg gctcccacgc
     2881 cgtgagcagc gccgacggcg cgtaatcgaa gagcgtcgcc ggctccggct cgaccagccg
     2941 cacgcacgcc tcgaccccgg ggaaggtgcg gccggaggcg agcgcttcga ccttctcggc
     3001 gagatcgtcg gcgcgcaacg cggcggcgtg gccgcggcgc tcgatccgcc gcgcctccag
     3061 ggcttcgtgc aggcgccgga gggccgccgc gtcccatggg tactcccgca ccggcgggac
     3121 gcgggccgat gcgaccgctt cccccgatcg ctgcgtcgcc gggtcgaagc ggcggagcga
     3181 ctccacctcc tccccccaga actcgacccg cagcggcagg gcgccgtcgg gagggaagag
     3241 atcgatgatg ccgccgcgcc gcgagaactc cgccatcgac tccacgaggt cggcccggtc
     3301 gtagccggcg gccaggagcg cctccgacag gtcgtcgggg cgcagcgact gcccggcgcg
     3361 caggtcgaag gtcgcgatgt cgagggtgcg aggcggcgcc agcgggtaga tgagggcccg
     3421 caccggcacg acgacgatcg cctctcccgg ctcgcgcagc gcccgcagcg cctcgacccg
     3481 gtcgcacgcc acctggagat gcggcgtcat ctcctggtac gggtcggcct cgagcgacgg
     3541 gaagaggcgc acgcgcacgc cgcgctcccg cagggccgag gcggcgaact cgaggtcgtg
     3601 ccggaacggc tccgactcgc gctcccccgg gacgaggagg acgatcttcc ccccgggcgc
     3661 gcggtcgagg agggacggca ggagcaccgc cgccgccggc ccggacagcc cggcgagggt
     3721 cagccgcccg tcgggcgtgt cgaggcggcg cagcagctcg gcgagcgccg catcccgcct
     3781 cggaaggtcg agaagggcgc ggacgtcccg gttcaccgcc gccgatcccg tcccgccgcc
     3841 gcccgcatcg cggtccgggc gcgctgccca gcccgcaccg ctgtccgggc gcgcgagacg
     3901 atacgggagg tggaccgccc atggctcagc cggacgcgga ccacccgccc gcccagggcg
     3961 cgcaccgtgt cggcgccgac gatctggtcg gcgcggtagt cggccccctt cacgaggacc
     4021 gccggccgga tcgcggcgat gatcgccgcc ggcgtgtcct cgccgaacgc caccacgaca
     4081 tccaccggct tcagcccggc gagcatctcc atccgttccc ggagcggcac gatcggccgc
     4141 ccgttcccct tgagccgccg caccgacgcg tcgctgttca ccccgacgac caggacgtcc
     4201 cccagggcgc gcgcacgctc gagcaggtcg aggtgaccga cgtgcaggat gtcgaacacg
     4261 ccgttggtga agacgacgcg tgcgccgcgc cgccgcagcc ccgcggcgtg cgccgccgcc
     4321 ttccggcgtc ccacgattcg ccccattggg acccgccctg cccgcaccgt cccctcccca
     4381 acccgcttca tgacgccggc gcgccgtccc tttgcggcgc gagacgatcg taccgcccgc
     4441 ggtgctcgcg ccacaggacc tgcaggacgt ctcgcagcat cgccagcgaa tccttgacgg
     4501 cgtgcacctt cgagccgggg gcgtcgcgcc agaggaccgg gacctcccgg acccgcaacc
     4561 ccgccttgcg cgccagcatc aggatctcga cgtcgtaggc gaaccgctcg acccgcgcgg
     4621 cgcggaagag aggtgccgcg gcctcgcgcc gcatcagctt gaagccgcac tgcgtgtcgc
     4681 ggtacggcag gcgggtcagc atccggacga tccggttgaa gatgcgcccc atcgtctgcc
     4741 gccagatcgg ctggcgcagc tccacccgcg atcccctgag gccgcgcgag ccgatggcga
     4801 tgtcgcactc ggaggcggcg accgggttca agaggaccgg cagctcgtcg atcggcgtgg
     4861 agaagtcggc gtcgctcaga aggacgtacg tgccggccgc gaggagcacg ccgtgcttga
     4921 gcgagtaccc cttgccgcgg ttgaccccgt tgcgcagcag ccgcagcgcg gatccggcgg
     4981 cggcgccggc cggccgggcg accgcgccgg ccgcgaacgc ctcgacgatg gcggcggtgt
     5041 cgtcgcggct gccatcgtcc acgacgagaa tctcgaccgg tcccgggaga cgccccggga
     5101 cggcgagcca gacgtgcagg cgctccaggc tgtcgcggat cctcccggct tcgttgtacg
     5161 ccgggatgac gatcgacaga gaaggcgccg tcaggggccc tctcggcggt ggcgggactt
     5221 cccgacttcg gcgcggagga gggctttgcg catcgcctgc tgggcgcgct cgaagtcggt
     5281 ctccggcgcg gcgctcttca ggcgctgctc cgcccgctcc ttggcctgct cgg
//
LOCUS       DASRIQ010000264         3771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_882594, whole genome shotgun sequence.
ACCESSION   DASRIQ010000264 DASRIQ010000000
VERSION     DASRIQ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882594"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFD06_06520"
     CDS             <1..925
                     /locus_tag="VFD06_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN03226.1"
                     /translation="AVRLDVGTEEHYPYGWTFPMGLASGDFDGDARNDLVVAVSGLHD
                     PGVPGTVRVLRGLPGGVFAPYATVGQTEFSASVAVADLDADGDADIAVADVASYTAQR
                     PGGLFMLLNDGSGAFLQSSILASGIGPYFVQVADMTGDGIPDLVASANAGYTAILAGL
                     GGGAYAEPIFFGTPGTVVPGDFDGDGFQDLLVVSYLSSFVMLNQTPSEVPLQIDAAIS
                     FTKQLGRGSGTLTFTTNVETDLVGFNVLEITAQGTTSVNRALIPCEECTSGLGATYTF
                     LVPRHRSGRNFYVEAVHAGGGTELFGPARRE"
     gene            962..1468
                     /locus_tag="VFD06_06525"
     CDS             962..1468
                     /locus_tag="VFD06_06525"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZN03227.1"
                     /translation="MVGRAEGPLGPLVAGVDDGGVWFLEFGGGGRSDAHLEALGRRLD
                     RPLVRRRHPLLDRLRGELEEYFDGRRRVFEVPVRLEGTPFQLRVWHELTKIPYGEVRS
                     YLQIARGLGDPQAVRAVGGANGSNPISIVVPCHRVIGADGSIVGYGGGLERKRWLLAH
                     ERGERPLF"
     gene            1565..2008
                     /locus_tag="VFD06_06530"
     CDS             1565..2008
                     /locus_tag="VFD06_06530"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HZN03228.1"
                     /translation="MKVVAQRRLTAAGLLLLSGSFAGLAADTPPAAPAKGDLWEVVSQ
                     MSMEGMPMQFPPQKTTVCSAKEWTAPPGANDEKRKCTSSDFKMTGPKATWKVHCDGPP
                     AMDGTGEMTRDGADAFAGSIKFTSTEAAMNVKLTGKRLGDCDNPK"
     gene            complement(2019..>3771)
                     /locus_tag="VFD06_06535"
     CDS             complement(2019..>3771)
                     /locus_tag="VFD06_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZN03229.1"
                     /translation="YFSWAAWATVAARPGKDYTYTNNWPYEPLVGNRPSTSTYLWSGL
                     SLVALLGGLGLILLIFGKFDFLGWRGAPGAERQDLGQDALARWTLTPSQRVTGLYFAV
                     VAILFLLQTLAGGALAHYRVESGAFYGIDLASFLPYNLLRTWHLQLAIFWIATAWVAG
                     GLFLAPLVGGAEPRGQKTGALVLLGALAVVVFGSLTGEFLGINNQLGNLWFWLGHQGS
                     EYLDLGRLWQVLLAVGLVGWLVLMFRALAPAMRRGGKDSLASLFLYAAVAIPVFYLPA
                     LFYGPHTNYAVIDNWRFWIIHLWVEGFFELFATVLVAVIFFQMGMVSGRTATRVVYLD
                     AILYLGAGVVGTGHHWYFTGQGTLNMGLAACFSAMEVVPLTLLTLDAWDFIRLQNRAE
                     GGAHPAAARHRWAIAFLIAVGVWNFVGAGVFGFLINLPVISYFEMGTGLTTNHGHAAM
                     FGVFGMLALGVLVFCLRASTPDARWAKSEPLVRVGFWGLNIGLALMCLIDLFPAGILQ
                     LVDSLQHGYWHARRLTFTMTGLFHTLEWLRMFADMIFLVFGVCPIVAATLRATLGRGA
                     VGGREPAPLQAAPSPTV"
BASE COUNT          640 a         1296 c         1302 g          533 t
ORIGIN      
        1 ggcggtccgc ctcgatgtcg gaaccgagga gcattacccc tatggatgga ccttcccgat
       61 ggggctggcg agcggtgatt tcgacggcga cgcgcgcaac gacctcgtcg tcgcggtgag
      121 cgggctccac gacccgggag tgcccgggac ggtccgggtg ctccgcgggc tgccgggagg
      181 ggtcttcgcc ccgtacgcca ccgtgggcca gaccgagttc tccgcttcgg tcgccgtcgc
      241 cgacctcgac gcggacgggg acgccgacat cgccgtcgcc gatgtggcct cctacacggc
      301 gcagcgtccc ggcggcctgt tcatgcttct caacgacggc agcggcgcgt tcctgcagtc
      361 atccatcctc gcctcgggca tcggaccgta tttcgtgcag gtcgccgaca tgacgggaga
      421 cgggatcccg gatctcgtcg ccagcgcgaa cgccggctat accgccatcc tggcagggct
      481 cggcggcgga gcctacgcgg agccgatctt cttcgggacg ccggggaccg tggttcccgg
      541 ggacttcgac ggcgatggat tccaggacct gctggtcgtc tcgtacttga gttcgttcgt
      601 gatgctcaac cagacgccgt cggaggtgcc gctgcagatc gacgcggcca tctcgttcac
      661 caaacagctc ggtcgcggct ccggtacgct cacgttcacg accaacgtcg agaccgacct
      721 cgtcggcttc aacgtcctgg agatcaccgc gcagggcacc acgtccgtca accgggccct
      781 gatcccctgc gaggagtgca cgagcggcct cggagcgacc tacacgttcc tggtccccag
      841 gcaccggagc ggcaggaatt tctacgtcga ggcggtgcac gccggcgggg gcaccgagct
      901 gttcggtccg gcgcgccggg agtagccgcg cgccgcccgc cacatggccg gcgaaccgct
      961 gatggtcggc cgggccgaag gccccctcgg ccccctcgtc gccggggtcg acgacggggg
     1021 ggtctggttc ctggagttcg gcggcggggg gcgcagcgac gcgcatctcg aggcgctcgg
     1081 ccggaggctc gacagaccgc tcgtccggcg ccggcatccc ctgctcgacc ggctgcgcgg
     1141 ggagctcgag gagtacttcg acggccggcg gcgcgtcttc gaggttcccg tgcggctcga
     1201 agggacgccg ttccagctcc gcgtctggca cgaactgacg aagatcccgt atggcgaggt
     1261 acggtcctat ctgcagatcg cccggggcct cggcgatccg caggcggtgc gcgcggtcgg
     1321 aggcgcgaac ggcagcaacc cgatctccat cgtcgtcccc tgccaccggg tgatcggcgc
     1381 ggacgggtcg atcgtcggct acggcggcgg cctggaacgg aagcgctggc tcctggcgca
     1441 cgagcgcggc gaacggccgc tgttctagtc tcgcggcatt gcgacacgca cctgcgcgcg
     1501 tgtatcgttc cggccacagt cgcccggtcc cacacaacgc tgttcctctc gtggaggtgg
     1561 atggatgaag gtcgtggcgc agcgccgcct gacggcggca ggcttgcttc tgctttccgg
     1621 ttccttcgcc ggcctcgcgg ccgacacgcc gccggccgcc ccggcgaagg gggatctgtg
     1681 ggaggtcgta agccagatgt cgatggaagg gatgccgatg cagttccctc cccagaagac
     1741 cacggtctgc tccgcgaagg aatggacggc gccgccgggg gccaacgacg agaagcgcaa
     1801 gtgcacgtcc tccgacttca agatgaccgg accgaaggcg acctggaagg tgcactgcga
     1861 cgggccgccc gccatggacg gcacggggga gatgacccgc gatggggccg acgccttcgc
     1921 gggatcgatc aagttcacct ccaccgaggc ggcaatgaac gtcaagctca ccggcaagcg
     1981 cctcggcgac tgcgacaacc cgaagtagcg tcgtctcgtc agacggtcgg gctcggagcc
     2041 gcctgcagcg gcgccggctc gcgaccgccg acggcacccc gtccgagtgt tgcacgcagc
     2101 gtcgcggcga cgatcgggca gacgccgaag accaggaaga tcatgtcggc gaacatccgc
     2161 agccattcga gcgtgtggaa caggccggtc atggtgaacg tcagccgccg cgcgtgccag
     2221 tagccgtgct gcagtgaatc gacgagctgc aggatcccgg ccgggaacag gtcgatcaga
     2281 cacatcagcg ccagcccgat gttgagcccc cagaatccca cccggacgag cggttcgctc
     2341 ttggcccagc gcgcgtcggg agtcgaggca cgcaggcaga acaccagcac cccgagcgcc
     2401 agcatgccga atactccgaa catcgccgcg tgcccgtggt tggtggtcag cccggtcccc
     2461 atctcgaagt aggagatgac cggcaggttg atcaggaagc cgaacacgcc ggcgccgacg
     2521 aagttccaga caccgaccgc gatcaggaag gcgatggccc agcgatgccg cgccgccgcg
     2581 ggatgcgcgc ccccctcggc ccggttctgc aggcggatga agtcccaggc gtccagcgtc
     2641 aggagggtca gcggcaccac ctccatggcc gagaagcacg cggccagccc catgttgagc
     2701 gtcccctggc cggtgaagta ccagtggtgc cccgtgccga cgacgccagc cccgaggtac
     2761 aggatggcgt cgaggtagac gacgcgcgtc gcggtgcggc cgctcaccat ccccatctgg
     2821 aagaagatga ccgcgacgag caccgtcgcg aacagctcga agaagccctc cacccacagg
     2881 tggatgatcc agaagcgcca gttgtcgatg acggcgtagt tggtgtgcgg cccatagaac
     2941 agcgccggca ggtagaagac cgggatcgcc accgcggcat agaggaagag tgacgcgagc
     3001 gagtccttcc ctccgcgccg catcgccggc gcgagcgcgc ggaacatcag tacgagccac
     3061 ccgacgagcc cgaccgcgag cagcacctgc cacaggcggc cgaggtcgag gtattccgat
     3121 ccctggtggc ccagccagaa ccacaggttg ccgagctggt tgttgatccc gaggaactca
     3181 cccgtgaggc tcccgaagac gacgacagcg agggcgccga ggagcaccag cgccccggtc
     3241 ttctggccgc gcggctccgc ccctccgacc agcggcgcga ggaagaggcc gccggcgacc
     3301 cacgcggtcg cgatccagaa gatcgccagc tgcaggtgcc aggttctcaa aaggttatat
     3361 gggaggaagc tcgcgaggtc gatgccgtag aacgcccccg actcgacgcg atagtgggcg
     3421 agcgcccctc ccgccagggt ctgcagcagg aacaggatcg ccaccaccgc gaagtacagc
     3481 ccggtgaccc tctggctcgg ggtgagcgtc cagcgcgcca gggcatcctg accgagatcc
     3541 tggcgttccg cccccggcgc gccacgccag ccgaggaaat cgaacttccc gaagatgagc
     3601 aggatgagcc cgagccctcc gagcagggcg acgaggctga ggccgctcca gagatatgtg
     3661 gatgtgctcg gccggttccc gaccagcggc tcgtacggcc agttgttggt ataggtgtag
     3721 tccttccccg gtcgtgccgc gaccgtcgcc cacgccgccc aggagaagta g
//
LOCUS       DASRIQ010000265         5074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1163845, whole genome shotgun sequence.
ACCESSION   DASRIQ010000265 DASRIQ010000000
VERSION     DASRIQ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163845"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="VFD06_06540"
     CDS             complement(<1..1727)
                     /locus_tag="VFD06_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HZN03230.1"
                     /translation="MRPSARLLLACLLAMIPGLMWPGRPTRAQDGPRPGWFGSARHCI
                     DDREFELTRLSGSGAETWQAPNRAHRIRTTFSPERVRVVPRNGQAFVPWTLEWSVAVW
                     ERDGVAWPASRATLVPDGRRVEFRRDAAPLEWYENTPRGLEQKFLIRAPRGPERPCRR
                     APFPERVQIDLRLGGDVSPRLRDGGQEIDFVTASGARALHLGPLRAVDARGRVLAARY
                     ESWERSDGRGLRYVVDVSGAVDPIRLEAIVNDSGWAAESEQPGASFGLSVATAGDVNG
                     DGYSDIIVGAPHFDAGQEDEGRVFAYFGSAAGPSASPDWSAEGDQAHALFGSAVAAAG
                     DVNGDGYGDLVVGAPRFDDGDEDVGGAFVYLGSPSGPGDSPVWMVEGTRPFAGFGTSV
                     ATAGDVNGDGRDDLLVGAPRHAEGGVEQGAAFLYLGSAAGPADQPAWVATGGQPGGRF
                     GERVAAAGDVNGDRRSDIAVGAPRQDDGDMEEGRVFVYLGSTTGLSAGPDWSAEIDQP
                     GALFGSSLAAGDVNGDGYGDLVVGAPFFEDHEDDEGHAFGYFGSAGGLGAGPEWVGEC
                     NQPSAGFGTS"
     gene            complement(1809..3614)
                     /locus_tag="VFD06_06545"
     CDS             complement(1809..3614)
                     /locus_tag="VFD06_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN03231.1"
                     /translation="MATIGRYIVERELGRGAMGRVCLATDPHLGRKVAVKTYVLPDGV
                     SPELAAQFHERFLREARAAASLSHPGLVTVFDAGEDEASSRPFIAMEFVDGRSLKERL
                     EEKGRLDAAWVLDMGSTLAEALGVAHRAGIVHRDIKPANILIREEDGAAKLADFGVAH
                     LKSSELTQTGTAPGSPGYMSPEQIQSGEVDGRSDLFSLAVVLYEALSGRRPFRGDDLV
                     ALTYSIAHETQVPLSRHLRDCPPALDQFFDRALSKDPTKRFGDAKEFRAALVAAAQAK
                     GPEASERTILEAESGATTPLPGLTIPLPPAAAGAAVGPEPAAKRPAPPAAGPARRGGR
                     RFAALSVAAIALLAAAGAAVWLGLGRPDLLRAGSRASKPATTAANVTTDPGTPLPRRE
                     DILAEVTPVPEPPPRRQPEPRRPAPATPPRPPSRVSVTLPAGTDIDVVLDTALSSAAS
                     FAGDAFTARVVHPVLDGDRVVIPAGSTVRGQVSAVMPAKRGLRDKGGSLTLSFDRLQM
                     PDGTGAALAASLTRAGGTSGKKTAGAVGGGAVGGALLGKILGGTSKDAAAGSVVGAAI
                     GTGVAAGTRGEDVDLSRGAPLTVQLGSPLTLSIRR"
     gene            3877..4890
                     /locus_tag="VFD06_06550"
     CDS             3877..4890
                     /locus_tag="VFD06_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZN03232.1"
                     /translation="MLAVFAGAWAAQLARPVLLPIVLAVLIAIVLTPLVGLLGMLRLP
                     APAGAFIVVALFAGGIGYGVYALSEPAADWLERAPETMREVEIRLRALKASMAEARAA
                     AESVEKMASVDGEAPPAEVTVREPSITARAVRVARTLLLRILEVLLLVYFLLAFGGPF
                     YRRLLRIPDGRRGKLRIVQITTEIQREVSSYLLTVSIINVGLGIATTLATSLLGMPNP
                     LLWGVMATVLNFVPYLGSAVTLIVLTIVAILTYDSLARALLVPLVFLGIATIEGQLVT
                     PIIVGRRMSLSPMVIVVALLVGDWMWGVVGMLIAVPVLAVFKILCSHDERLAPIAELL
                     GTD"
BASE COUNT          681 a         1904 c         1782 g          707 t
ORIGIN      
        1 gacgtcccga acccggccga cggctgattg cactctccca cccactccgg tcccgctccc
       61 agtccgccgg ccgaaccgaa gtagccgaag gcgtgccctt cgtcgtcctc gtgatcctcg
      121 aagaacgggg cgccgacgac gaggtcgccg tagccgtcgc cgttcacgtc ccccgctgcc
      181 agcgaggatc cgaagagagc gcccggctga tcgatctccg ccgaccagtc gggaccggct
      241 gacagccctg tcgtcgaacc gaggtagacg aagacgcgtc cttcctccat gtctccgtcg
      301 tcctggcgcg gcgctccgac ggcgatgtcg gatcgtctgt cgccgttcac gtcccctgcc
      361 gcggcgaccc gttctccgaa tcgcccgccc ggctgacctc cggtggcgac ccacgcaggc
      421 tggtccgccg gtcccgccgc cgaaccgagg tacaggaacg ccgccccctg ctcgacgccc
      481 ccctcggcgt ggcgtggggc gccgacgagc aggtcatcgc ggccgtcgcc gttgacatcc
      541 cccgccgtcg ccaccgacgt cccgaacccg gcgaacggcc gggtcccttc caccatccac
      601 acggggctgt cccccggacc ggagggcgat ccgagataga cgaacgcgcc ccccacgtcc
      661 tcgtcgccgt cgtcgaagcg cggcgccccc acgacgagat cgccgtatcc atcaccgttc
      721 acgtcccctg ccgcggcgac ggcggatccg aacagcgcgt gcgcctgatc cccctccgcc
      781 gaccagtcgg ggctcgccga cggcccggcg gcggagccga agtaggcgaa cacgcgtcct
      841 tcgtcctcct gacctgcatc gaaatgaggg gcgccgacga tgatgtcgga atacccatcg
      901 ccgttcacgt caccggcggt cgcgaccgac aggccgaagg aggcacccgg ttgttccgac
      961 tctgccgccc agccactgtc gttgacgatc gcctcgaggc gaatcggatc gacggcgccc
     1021 gacacgtcga ccacgtagcg caggccgcgt ccgtccgacc tctcccacga ctcgtaccgt
     1081 gccgcgagga cacggccccg ggcgtcgacg gcgcgcagcg ggcccaggtg cagcgcgcgc
     1141 gccccgctcg ccgtgacgaa atcgatctcc tggccgccgt cgcgaagccg cggcgacacg
     1201 tcgccgccga ggcgcaggtc gatctggacg cgctccggga acggggcgcg gcgacacggc
     1261 cgctccggcc cccgcggagc ccggatcagg aacttctgct cgagaccgcg cggcgtgttc
     1321 tcgtaccact ccagcggcgc cgcgtcccgg cggaactcga cgcgcctgcc gtcgggaacg
     1381 agcgtggcgc gcgacgccgg ccacgccacg ccatcacgct cccagacggc gaccgaccat
     1441 tccagggtcc aggggacgaa ggcctggcca ttccgtggga cgacgcggac ccgttcgggc
     1501 gagaacgtgg ttctgatgcg atgcgcccgg ttcggagcct gccatgtttc ggcgccgctg
     1561 ccggacagcc gggtcagctc gaattcgcgg tcgtcgatgc agtgacgggc agagccaaac
     1621 catccggggc gcggcccgtc ctgtgcgcgc gtcgggcggc ccggccacat caggccgggg
     1681 atcatcgcca gaaggcaggc gaggaggagg cgcgcggatg ggcgcatcgg ccctcgttgt
     1741 acggccgggg ccacgaagcg tcaagcgaat tcgcggcggt ggggtctgcg ggcgggcgat
     1801 ggaagcggtc agcgccggat cgagagggtc agggggctcc cgagctgaac ggtcagcgga
     1861 gcgccgcggg acagatccac gtcctcgccg cgcgtgccgg ccgcgactcc ggtgccgatc
     1921 gccgccccga ccaccgaccc ggcggcggcg tccttcgacg tgccgccgag gattttcccg
     1981 agcagcgcgc cgccgacggc gccgccaccc accgcccccg ccgtcttctt tccggacgtt
     2041 ccgccggccc gggtcagcga cgcggcgagc gcggctcccg tcccgtccgg catctgcagc
     2101 cgatcgaacg acagcgtcag cgatcccccc ttgtccctga gccctctttt cgccggcatg
     2161 accgcgctca cctgtccgcg gacggtgctc ccggcgggaa tgacgacgcg atcgccgtcg
     2221 aggaccggat ggacgacgcg ggcggtgaag gcgtccccgg cgaagctcgc ggccgaggag
     2281 agcgcggtgt cgaggacgac gtcgatgtcg gtcccggcgg gaagcgtcac gctcacgcgc
     2341 gacggcggcc ggggcggcgt cgccggcgcg gggcgccgcg gctccggctg gcggcgcggc
     2401 ggcggctccg ggaccggagt cacctccgcg aggatgtctt cccggcgcgg cagcggcgtc
     2461 cccggatcgg tggtcacgtt cgccgcggtc gttgccggct tcgaggcgcg gctccccgcg
     2521 cgcagcaggt ccggccgccc gagtccgagc cagacggcgg cgcccgccgc ggcgaggagc
     2581 gcgatcgcgg cgaccgagag cgcggcgaag cggcggccgc cgcgccgagc gggacccgcc
     2641 gccggcggcg ccggtcgctt cgcggcgggc tccggtccga cggccgcacc ggccgcggca
     2701 ggcggaagcg gaatcgtcag cccggggagc ggcgtggtcg cgccgctctc cgcctcgagg
     2761 atcgtccgct ccgaagcctc ggggcccttc gcctgcgccg cagcgacaag cgccgcgcgg
     2821 aactccttcg catccccgaa gcgcttcgtc ggatccttgg agagggcccg atcgaagaac
     2881 tggtcgagcg ccggcgggca gtccctgaga tgccgcgaca gcggcacctg ggtctcgtgc
     2941 gcgatagagt aggtcagcgc cacgagatcg tcgccacgga acggccgccg gccggacagc
     3001 gcctcgtaca gcacgacggc gagcgagaac agatcgctgc ggccgtccac ctcaccactc
     3061 tgaatctgct cgggcgacat gtagccgggg gacccggggg cggtgccggt ctgggtcagc
     3121 tccgacgact tcaggtgggc gacgccgaag tcggccagct tggctgcgcc gtcctcctcg
     3181 cggatgagga tgttcgccgg cttgatgtcg cgatggacga tgccggcccg gtgtgcgaca
     3241 ccgagcgcct cggcgagcgt cgaccccatg tccagcaccc aggccgcgtc gagccgcccc
     3301 ttctcttcga gccgctcctt caggctgcgg ccgtccacga actccatggc gatgaaaggc
     3361 cggctgctcg cttcgtcctc accggcgtcg aacacggtga cgagcccggg atgggacagc
     3421 gacgccgcgg cgcgcgcctc gcgcaggaag cgctcgtgga actgcgccgc cagctccggc
     3481 gagacgccat ccggaaggac gtaggtcttc accgcgacct tgcggccgag gtgcggatcg
     3541 gtcgccaggc acacccggcc catcgcgccg cggcccagct cacgctcgac gatgtaccgg
     3601 ccgatcgtcg ccatcgaccc gaatataggt gcatgatccc ggtggacaac cgggctcccg
     3661 ggggcgtcgc cctgccgacg ccgtctcccc ggggacgtga ccgggaactg gacggaggat
     3721 gaacctggga acccgacgac gatgagcgcg accaggcgcg ggccggaaga tccgcctcgc
     3781 gcggaccgga ccggagcggc ggccgagcgg cctgtgctac caccccccga cgcggtggcc
     3841 tggtgggaga cccggcccgg ccggatcgga ttggggatgc tcgccgtgtt cgccggcgcc
     3901 tgggcggcgc agctggcgcg cccggtgctc ctgccgatcg tcctggcggt cctcatcgcc
     3961 atcgtcctca ccccgctcgt ggggctgctc ggcatgctcc gcctccccgc cccggccgga
     4021 gccttcatcg tcgtcgccct gttcgccggc gggatcggct acggcgtcta tgccctgtcg
     4081 gagccggccg ccgactggct ggagcgggcg ccggagacga tgcgcgaggt ggagatccgg
     4141 ctgcgcgcgt tgaaggcctc catggcggag gcgcgcgccg ccgccgagtc ggtcgagaag
     4201 atggccagcg tggatggcga ggcgcctccg gcagaggtca cggtccgtga gccctccatc
     4261 accgcgcgcg ccgtgcgggt ggcgcgcacc ctgctgctcc ggatcctcga ggttctgctc
     4321 ctcgtctatt tcctgctggc cttcggcggc cctttctacc gccggctgct gaggatcccg
     4381 gacgggcggc gcggcaagct gcggatcgtg cagatcacca ccgaaatcca gcgggaggtc
     4441 tcgagctacc tcctcaccgt gtcgatcatc aacgtcggcc tcggcatcgc cacgaccctc
     4501 gcgacgtcgc tcctcgggat gccgaacccg ctgctgtggg gggtgatggc gaccgtcctg
     4561 aacttcgtcc cctatctcgg aagcgcggtc accctcatcg tgctgacgat cgtcgccatc
     4621 ctgacgtacg actctctggc gcgggcgctg ctcgttcccc tggtgttcct cgggatcgcg
     4681 acgatcgagg gacagctcgt cacgccgatc atcgtcggcc ggcgcatgtc gctcagcccc
     4741 atggtgatcg tggtggcgct gctcgtgggg gactggatgt ggggggtcgt cgggatgctg
     4801 atcgccgtgc cggtgctggc ggtcttcaag atcctctgct cgcacgacga gcgcctggcg
     4861 ccgatcgccg agctcctcgg gacggactag cagccgctcc gccgggtgtc gaccggaacg
     4921 gctccttcag caccaccttc accccctgaa tcgtgccgac gacggcgtgc cgcaccttgt
     4981 cggcggcggt cgccccgacc tcgctcgccg cgcgcatcgc tccggtggcc gcggcgccgg
     5041 ccgcctcgga ggcgtcgagc ccgatctcct tcgc
//
LOCUS       DASRIQ010000266        12823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_638184, whole genome shotgun sequence.
ACCESSION   DASRIQ010000266 DASRIQ010000000
VERSION     DASRIQ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638184"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFD06_06555"
     CDS             <1..462
                     /locus_tag="VFD06_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03233.1"
                     /translation="AAWRPLVRGLREPSFAFDSMSLPDGLYRIRLDVSDAPSNPGDHA
                     RVASQTSEAFLVDNTPPSVQVTARKGPKGGSTTIEVTATDATGPLARAEMSLDAARFA
                     PLSAADGASDSRAESYAQTLEGLRPGEHLVIVRVVDLLGNVGSGKALVTTE"
     gene            459..2180
                     /locus_tag="VFD06_06560"
     CDS             459..2180
                     /locus_tag="VFD06_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN03234.1"
                     /translation="MIRRSGGAAAVRVAVPRPRRTCVVLCVLLLTAALVPSCRRQQAL
                     GGRFVYSMQAEPTTLNFVAGSDFPSTLVERLISDFLVDHDTSLAIVPRLAESWAWSED
                     GRTLTFRLRPGVRFHDGVPLTSADVLFTYERLIDPKSKAHGRLDGFLSVERVAAPDPG
                     TVVVTYREPYAPALRAWQVPILPAHRYRGADFETAPENRSPIGAGPFRFVAWETGRRI
                     VLRANEDYWGGRPALDEFVFQFQPSLDTALLALLSGEVDYAKLTASAWEARRGDSEFA
                     SRYRVLNFTPLFFYYIAWRGDGSNPFFADPAVRRALGLALDRQGYVRSVLHGLGEPIA
                     SPFAPLLPSQDAGRPPEQEAAEAGPLRFDPDEAARLLDMAGWRIDRRTGQRARGGLPF
                     RFTLLVFAGSEDHIQFAQVAQDALARIGVTMRVERLDWPALYARLKSGAFEAALSGFT
                     PPPDPDSLYGMLHSSQIGGGQNYAAYRDPEVDRWMDEGRRTPDSPARTALYRRVEERL
                     ATTQPYACLFAPTVVGAINARFEMVHPSPMGIIGHVPGALAIRLATGADTSPAASPGG
                     SRTGAGG"
     gene            2186..3169
                     /locus_tag="VFD06_06565"
     CDS             2186..3169
                     /locus_tag="VFD06_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN03235.1"
                     /translation="MAGFVARRVLLAGPTLLGISFLAFFVLSVLPGDPLAARIDADTP
                     LSAEAEARLRAELRLDRPVLVRYAEWLVAAARGDLGRSVHDGRPVAGILREAAPWTLL
                     LNLSALATIYGLALPLGLLQARRPRSAWARLSEGGLILASVIPPFVAALLLQALFAVR
                     LGWLPLEGTGEPRSGFASPGGLLSHLALPALCLALAGWAFATRYARGAFAEAVPASAL
                     AAARARGLRGPDLLKHFALLLALPLVWLLGGIVPSLLSGSVIVEEAFSWPGLGRLMVQ
                     AVLGRDTPLAMALLLLSAGSVLAAQLLIDILYPALDPRLRSEGGETARAPD"
     gene            3156..4091
                     /locus_tag="VFD06_06570"
     CDS             3156..4091
                     /locus_tag="VFD06_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN03236.1"
                     /translation="MHRTDPAAVAGAPGAASASAGVPSERPPGARSRRAGLALLGGLG
                     LLALAAPRLASEDPLLDLRLGSARLTVPAPVPWSERRVRLEESLAPPSRRHLCGTDLL
                     GRDVLARLLHGTRASLTIGFGATLGALLLGLLLGGTAALRPGPVDLMLGRVIEILGSF
                     PPLLLALSLVAASGQRGILPMIAGIAAGRTAAAARFARAETLRLQGTSFWLAARASGA
                     GPWQTARRHLLPMLEAPLLVQAAFGIAQAILLESTLGFLGLGVEPPIPSWGQMIGEGR
                     GIAAVAWWTIAYPAAALAVTLALLLRLPKVDSRVW"
     gene            4218..5393
                     /locus_tag="VFD06_06575"
     CDS             4218..5393
                     /locus_tag="VFD06_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03237.1"
                     /translation="MHWPRTPVESLSGFQPGFCPRRQCSEHLRSKPGYRFRRLGFYST
                     SRRWRIPRFVCLTCRGSFSRQSFAVSYYRKRPELLLPVAAGLVAGSAHRQLARTLGCA
                     PSTVTRIASRLGRHAILLLALARDDLLGKVEESFVLDHFETFEITQDYPFGVATLVGA
                     RSWYVYDLDPAPHGRTGRMSHGQKDRLRERPQRPRRGGYLGSSRRVFERVLALAPASG
                     NAVVRGDGHAAYDRAAAEQHRLSRDGMPQLVLERYPNPARGSKGSPRSAEARLRDARL
                     FPVDLLHKILRHSLAHQRRETIAFGRRLNAILERLFLAAVWRNFVKRRSERFSRSGTP
                     AMRLGLARDRWDWQRVLARRIFPGRTPTPPTWALLYRRLWTTPLYPTNSTHALSRAF"
     gene            complement(5520..12758)
                     /locus_tag="VFD06_06580"
     CDS             complement(5520..12758)
                     /locus_tag="VFD06_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN03238.1"
                     /translation="MVESPGPDGNLATAADNRFYDGNRYDGGRNNANFDQWSLGRTST
                     TPVEKHDKRNNTEAVHLSADTNIDFLFDESPLYTGLWRVTVKRGLGGAIAGQITITAP
                     TVGQDPDQNEDDNNNGRLDAGEDNNANGLLDQLGQPYSLVVSGPVFLAEAAPPAGPSI
                     FPSSMVSWDANRYGCSSNARLLVLDTTGTATPANVQSSTSYLVQNAAGATTDSESSFT
                     YAAGAATGSFTSAAIPVRLGGPAVANNGILEADTGSLISATYARLGQAAVTAKALVSC
                     SPDLIQGAFTAFAGNALGNQFAVAGGCDNDDNLDAGEVVTYGIALQNRSRVNNYADLV
                     ATLTPSGPGAAAIRVLDSPKNMGSFPGNGVNGVFFHVFVDPTIANGLAVANRVVDMTL
                     SLDSLVRGTRISRQTYTFKHAINSDRQEFFYSTDFPNGGRQVRDINRNLVVDPPDTTD
                     PFLGFIVTREDVTFSSLFSGTGAPVGQFTNQLGEDLDNNGIFSGVERNVVPDVDGGGV
                     AILNKGVLNSNNPADPLHRVPWNFDNNNGGWIPFRHPGSNAAGVNVNPLWEYKTSGLC
                     GFQSTGGLNKFGIWHTGDGDPTTPGGAATACDNHTQPNDPNTPEKVELLMDVLESPIV
                     AKVNQNNDARGFAFTVEFQRFAYNENIQLNDGYAGGGVNIDNDIDSDNANSLLGQQMD
                     SYYTRRTGGWPYGMFRDAGSYFPGDGIDPATTAPFQRTFGPFTNPNASVNLDGDETGF
                     SGHTQDTNADSSTPIPTTIPDFLAYPVPNAPVIGICDGGTQNDDTCDPGNGADPCIVG
                     GGVCTAAVNTVNGPVRNWDATLIGYEGGFASLIDNGPQENFYFFFPGGASNRWQIGVG
                     FWAMEGTAGDTDYGKSMDDAVFEWKEYHPQDEAALGAQPACTRFNNASLNFPQGGQCA
                     TLTADRTSLYECDEGMEITVYDAKCRTIGAGNTVPVGGACTTNTPCGAGGLCSATLPS
                     VQVQVVTDTDSVPVAAAGGQVRFPNAKLYTLNAVPGPGNAGLFRGTIIFSTTTNDPNH
                     AYTNPGSDGVFSVYYHDPICDGDFDGQAGEDNFNNVDGDGVADASDNCPQLYNPLQED
                     TDADGFGTFCDNCPTVDNADQGDANADGIGDACEFDDVDGDGLPNDADNCPDNRNPNQ
                     ADIEADGRGDLCDTLKTQGVTFGTSAGAADCVSGLCTVPGDARGDACVTDEDCIRSCR
                     TNICTQGNNGSAGGAQCYGGPNDGNACAPGGTDCGICVGGPTPGAPCAVAATCGAGGA
                     CNVGVCFNNFVSPTPKIGQACATHADCYNDLDRDGDGIVDALDNCTIADNGPLQGPNI
                     QTDSDGDGLGNVCDGDCANAVEVFRCRANGVACPVPESNQAACANAFGLGNVCGFYVQ
                     NSGSCSTVDDDFDADNIGDLGDSCATVFNAAIIAGTDRQRDSDRDGIGDACDPAGTFD
                     DAQDGLPDDVVAFNGNIVCRTQPLANLAILAADYRDTDGDIDAFPDTGENGRIRLTLR
                     NLGPNLTDATVVLTSSDSDVACITKPTLLVPADPGNTPPQALGQWQNGVTRVLGDFVN
                     NNPGFQFIASAGLNYTGPPAPIPTTNFCITVVANETLGTASPFCFSLLADLDPPGGPQ
                     PAFTLGNDGLNGTADDGTLVENFDVDKNGDGQITVKDTFLQATAPGTFRGYCSNAPNT
                     TCQTDNDCGGAPALCYMGSYIRGDATGVAVGTVAATSCGGYDTYGGANTYCQLDPDFP
                     MDWHVHCSPGAVNCPNLETGTCVGGCSYATPVGGQKSHSGSNSLHMGAHFDPADNLAG
                     DTTHFRTLQGYQSGPMNLALFPRPGDMELSFYHIARLMDNNGVGPNNERQCVDCGDVQ
                     IQLDGDGGANVDNWGFWDKLVPFQNVYDHKPNAFSVFGGYYCEFTPTDTGTAPPNPRG
                     VHETTCYPLGTWSHCGSTIGTVATATVDCAGPGEVDATGVGVWVQTKFNLSGYVGQRV
                     RLRWIAESWTFDENSSNYFEIGGTWAATQQDDGWWLDDIRITGTIQSQFTPNPDLAPS
                     PGAICPSDPCNQALGDGGTNVVLNITDLNGNVLDGVTTFPFAGQSIRVSAIDSTNPGG
                     CMNGVLEYEFSKDGVVKQAFGPKSFYLDAPESAANYGCRARCSTDFTCTSLVGGSIAV
                     NPYTGDGSDAIFANPPNQSLGVRYFRGTCTAGPNPLGGCNEAADCGVGGACSVTAATG
                     DDTTRYSVVTNAGGLPNGLVDLYRGTVATAKGSRNGQIWLLNHTDGAAFVQHLAFVAN
                     NPPGKQFANTLNQATDVNPAVGAVQYYLAVGHAPGGNSVNALGCANPGICGNAGWCSL
                     GASAGAPCNVAGDCPGGACTIRTTFCTTDGGSGDVGGCARHQVCVGGTNAGNLCLAAG
                     DCPGGTCPAIPASPAGTCFSVQSGMPSSGCLPPGSAKRLVDQAVVLAP"
BASE COUNT         1670 a         4424 c         4581 g         2148 t
ORIGIN      
        1 gcggcgtgga ggccgctggt gcgcgggctg cgggagccgt cgttcgcgtt cgactcgatg
       61 tcgctgccgg acgggctgta ccggatccgg ctggacgtga gcgacgcgcc gtcgaatccg
      121 ggggatcacg cgcgcgtcgc cagccagacg agcgaggcgt tcctcgtcga caacaccccc
      181 ccctcggtgc aggtgaccgc ccgcaaggga ccgaagggag ggagcacgac gatcgaggtc
      241 accgccaccg acgcgaccgg cccgctggcc cgcgccgaga tgtcgctcga cgccgcccgg
      301 ttcgcgcccc tgtccgccgc cgacggcgcg agcgattcgc gcgccgagtc gtacgcccag
      361 acgctcgaag ggctgcggcc gggcgagcac ctcgtcatcg tgcgggtcgt cgatctgctc
      421 gggaacgtcg gctccggcaa ggcgctcgtc accaccgaat gatccggcgg tccggaggcg
      481 ccgcggcggt gcgggtcgcg gtcccgcggc cccgccggac gtgcgtcgtc ctgtgcgtcc
      541 tgctgctgac ggcggcgctg gtcccctcct gccggcgcca gcaggctctt gggggccgct
      601 tcgtctattc catgcaggcg gagccgacga cgctgaactt cgtcgccggc tccgacttcc
      661 cttcgaccct ggtcgaacgg ctgatcagcg acttcctcgt cgatcacgac acgtcactcg
      721 caatcgtgcc ccgcctggcg gaatcatggg cctggtcgga ggacggccgg acgctgacct
      781 tccgtctccg cccgggcgtc cgcttccacg acggcgtccc gctcacgagc gccgatgtcc
      841 tcttcacgta cgagcgtctg atcgatccga agagcaaggc gcacggccgg ctcgacggct
      901 tcctgtctgt ggagcgggtc gccgcccccg accctggcac cgtcgtcgtg acctaccgcg
      961 agccgtacgc cccggcgctg cgcgcctggc aggtgccgat cctccccgcg caccgttacc
     1021 gcggggccga tttcgagacg gcgccggaga accgctcccc gatcggggcc ggtcccttcc
     1081 gcttcgtcgc ctgggagacc ggccgccgga tcgtcctgcg cgccaacgag gactactggg
     1141 ggggccgccc ggcgctcgac gagttcgtct tccagttcca accgtcgctc gacacggcgc
     1201 tcctggcgct gctctccgga gaagtcgact acgccaagct gaccgcgtcc gcctgggagg
     1261 cgcggcgcgg ggattccgag ttcgcgtcgc ggtaccgcgt cctgaacttc accccgctgt
     1321 tcttctatta catcgcgtgg cgtggcgacg gctcgaaccc gttcttcgcc gatcccgccg
     1381 tccggcgcgc cctcggcctg gcgctcgatc ggcaggggta cgtccgctcg gtcctgcacg
     1441 gcctcggcga gccgatcgcc tcccccttcg cgccgctgct tccgtcccag gacgcaggtc
     1501 gcccgccgga gcaggaggcg gccgaggcgg gacccttgcg tttcgatccg gacgaagcgg
     1561 cgcggcttct ggacatggcc ggctggagga tcgatcggcg cacggggcag cgggcgcgtg
     1621 gcggcctccc cttccgcttc acgctcctgg tgttcgccgg cagcgaggac cacatccagt
     1681 tcgcccaggt ggcgcaggac gcgctcgcgc ggatcggcgt gacgatgcgg gtggagcggc
     1741 tcgactggcc ggcgctgtac gcgcggctga agagcggcgc cttcgaggcg gccctctccg
     1801 gattcacccc cccgcccgat cccgattccc tgtacggcat gctgcactcg tcgcagatcg
     1861 gcggagggca gaactacgcc gcctaccgcg acccggaggt ggatcggtgg atggatgagg
     1921 ggcgccgcac ccccgattcc cccgcccgga cggcgctcta ccgccgcgtc gaggagcggc
     1981 tcgcgacgac gcagccgtac gcctgcctgt tcgcgccgac cgtcgtcggg gcgatcaacg
     2041 cccggttcga aatggtgcac ccctccccca tggggatcat cggacacgtc ccgggagcgc
     2101 tcgcgatccg cctcgccacc ggcgccgaca cgagtcccgc agcgtcgccc ggcgggtcgc
     2161 ggacgggggc gggaggctga acgcgatggc agggttcgtc gcgcggcggg tgctcctcgc
     2221 cggcccgacg ctcctgggaa tcagcttcct cgctttcttc gtcctgagcg tcctgccggg
     2281 ggatccgctc gccgcccgga tcgacgcgga caccccgctc tccgccgagg cggaggcccg
     2341 cctgcgtgcc gagctgcggc tcgatcggcc ggtcctggtc cgctacgcgg agtggctcgt
     2401 ggcggcggcc cggggcgatc tcggacgatc ggtccacgac gggcgcccgg tcgccggcat
     2461 cctgcgggag gcagccccct ggacgctcct gctcaacctc tcagcgctcg ccacgatcta
     2521 tggcctggcg ctgcccctgg gcctgctgca ggcgcgccgg ccccgttccg cctgggcccg
     2581 gctgtcggaa ggggggctca tcctggcctc ggtgatcccc cccttcgtcg ccgccctgct
     2641 gctgcaggcg ctgttcgccg tccggctcgg atggctgccg ctggagggga ctggcgagcc
     2701 gcgaagcggc ttcgcgtctc ccggcggcct gctctcgcac ctcgccctcc ccgccctctg
     2761 tctcgccctc gcgggttggg cattcgccac gcgctacgcc aggggagcct tcgcagaggc
     2821 ggtccccgcg tccgccctgg cggccgcccg ggcccgcgga ctgcgggggc cggatctcct
     2881 gaagcatttc gccctcctcc tcgcgctgcc gctcgtctgg ctcctcgggg ggatcgtccc
     2941 ctcgctcctg tcaggcagcg tcatcgtcga ggaggcgttc tcctggcccg ggctgggccg
     3001 gctcatggtg caggcggtgc tcgggaggga cacgcccctg gccatggcgc tcctcctgct
     3061 gtcggcgggg tcggtcctcg ccgcgcagct gctgatcgac attctctatc cagcgctcga
     3121 cccgcgactg cggtcggagg ggggagagac ggcgcgtgca ccggactgac ccggcggccg
     3181 tcgcaggtgc gccgggcgcc gcttccgcgt ccgccggcgt cccgtccgag cgcccccccg
     3241 gcgcccgctc ccggcgcgcg gggctggccc ttctcggcgg tctcggcttg ctggcgctcg
     3301 cggcgcctcg cctcgcgtcg gaggatccgc tgctcgatct gcgcctggga tcggcgcggc
     3361 tcaccgtccc ggctcccgtc ccctggtccg agcggagagt gcgtctcgag gaaagcctcg
     3421 cccccccctc ccgccggcac ctctgcggaa ccgacctgct cggacgggac gtcctggcgc
     3481 gcctgctcca cggaacgcgc gcctcgctca cgatcggctt cggcgccacc ctgggcgcgc
     3541 tcctcctcgg cctgctgctc ggagggacgg cggcgctgcg tccgggcccg gtcgatctga
     3601 tgctgggacg cgtcatcgag atcctcggct ccttccctcc ccttctcctg gccctgtccc
     3661 tcgtcgccgc atccggccag cggggcatcc tgccgatgat cgccggcatc gccgccggcc
     3721 gcaccgccgc cgccgcccgt ttcgcacgcg ccgagaccct ccggctgcag ggaacctcct
     3781 tctggctcgc ggcccgcgcc agcggggccg gaccctggca gacggcacgc cgtcacctcc
     3841 tgccgatgct cgaagcgccg ctcctcgtgc aggccgcgtt cggcatcgcc caggcgatcc
     3901 tgctggagtc cactctgggg ttcctcggcc tgggagtgga gccgcccatt ccctcgtggg
     3961 gtcagatgat cggcgaaggg agggggatcg cggcggtggc gtggtggacg atcgcctacc
     4021 cggcggcggc gctggccgtc acactcgccc tacttctccg cctccccaag gtggactcga
     4081 gagtgtggtg agcggcggcc gctcgagggg tcgaccggat gaccgcgggc ggacgcggtg
     4141 tcatggtgat ttggcccgcg acgcccgcaa gaccggcgtg atctcgacgg cacagcgttt
     4201 gatcccaccg cctggccatg cactggccac gcacgccggt cgagtccctg tcggggtttc
     4261 aaccaggatt ttgcccgaga cggcagtgct ccgagcacct gcggagtaag cccgggtatc
     4321 gcttccggcg gctcggcttc tattcgacgt cgcggcgctg gcgcatcccg cgctttgtct
     4381 gcctgacctg tcgcgggtcg ttttcccgcc agtcgttcgc cgtctcctac tacaggaaaa
     4441 ggccggagct cctgctgcct gttgcggccg ggctcgtggc tggctcggcc cacaggcagc
     4501 tggcccggac gctcggctgt gcgccctcga cggtcacgcg catcgcgtcg cgcctcggac
     4561 ggcacgcgat cctgctcctg gcgctggccc gcgacgacct gctcgggaag gttgaggaat
     4621 cgttcgtcct cgaccacttc gagaccttcg agatcacgca ggactacccg ttcggggtgg
     4681 cgacgctggt gggggcacgt tcctggtacg tgtacgacct cgacccggcg cctcacggaa
     4741 ggaccgggcg catgtcgcat gggcagaagg accgactgcg ggaacgaccg caacgaccgc
     4801 ggcgcggcgg ctacctggga tcgagccgtc gggtcttcga acgggtcctg gcgctcgcgc
     4861 ccgcatcggg aaacgccgtc gtgcgcggcg acggccacgc ggcgtacgac cgggcagcgg
     4921 cggagcagca ccgcctttcc agagacggga tgccgcagct cgtgctcgag cgctacccga
     4981 acccggcgcg cggctccaaa gggtcgccac gcagcgccga ggcccgcttg cgggacgcgc
     5041 gcctcttccc ggtcgatctt ctgcacaaaa tcctgcgaca ttcgctggcg caccagcggc
     5101 gggagacgat cgccttcggc cggcgactga atgccattct cgagcgactg ttcctcgcgg
     5161 ccgtgtggcg taacttcgtg aagcgccgca gcgaacgatt ctcgcgaagc ggcacgccgg
     5221 cgatgcggct cggtctcgcg cgcgatcgct gggactggca gagggtcctc gcgcgacgaa
     5281 tcttccccgg ccgcactccg acgccgccca cctgggcgct cctgtaccgc cggctctgga
     5341 cgacgccgct ctaccccacc aactcgaccc acgctctcag cagggctttc taagacaggt
     5401 cgccggcagc gaaccccggc acatcgagcg ggtccgcgaa ccacactctt ggcaccacca
     5461 tgaaaaaaaa gggggccccc gaaggggccc ccttgggtgt tacgccctga gttggggctt
     5521 tacggcgcca gcacgaccgc ctggtccacc agccgcttcg cgctgcccgg aggcagacag
     5581 ccgctggagg gcatgccgct ctgcacgctg aagcaggtgc cggcaggaga ggccgggatc
     5641 gccgggcagg taccgcccgg gcagtcgccg gccgcgaggc agaggttgcc ggcgttggtg
     5701 ccgccgacgc agacctggtg ccgcgcgcaa ccgccgacgt cacccgagcc accgtccgtg
     5761 gtgcagaagg tggtgcggat ggtgcaggcg ccgccggggc agtcaccggc cacgttgcac
     5821 ggagcgccgg cgctcgcacc gagggagcac cagcccgcgt tgccgcagat cccggggttg
     5881 gcgcagccga gggcgttgac cgagttgccg cccggggcgt gtccaaccgc caggtagtac
     5941 tgcaccgcgc ccaccgcagg attcacgtcc gtagcctggt tgagcgtgtt ggcgaactgc
     6001 ttgcccggtg ggttgttcgc cacgaacgcg aggtgctgga cgaacgccgc gccgtcggtg
     6061 tggttcagca gccagatctg gccgttgcgc gaccccttcg ccgtggcgac ggtgccgcgg
     6121 tagaggtcga cgagaccgtt cggtaggccg ccggcgttgg tcaccaccga gtagcgggtg
     6181 gtgtcgtcac cggtggcggc cgtcacgctg cacgcgccgc cgacgccgca gtccgctgcc
     6241 tcgttgcagc cgcccagcgg gttcggcccc gccgtgcagg tgccgcggaa gtagcggacg
     6301 ccgaggctct ggttcggcgg gttcgcgaag atcgcgtcgc tgccatcgcc ggtgtacgga
     6361 ttcaccgcaa tgctcccacc gacgaggctg gtgcaggtga agtcggtgct gcaacgcgcc
     6421 cggcagccgt agttcgcggc cgactccgga gcgtcgaggt agaacgactt cggtccgaac
     6481 gcctgcttca cgacgccgtc cttcgagaac tcgtactcga ggacgccgtt catgcagccg
     6541 ccggggttcg tcgaatcgat ggcgctgacc cggatcgact ggcccgcgaa cgggaaggtg
     6601 gtgaccccgt cgaggacgtt gccgttcagg tcggtgatgt tgaggaccac gttcgtcccg
     6661 ccgtcgccga gagcctggtt gcacgggtcg gacgggcaga tcgcgccggg gctcggagcc
     6721 aggtcgggat tcggcgtgaa ctgcgactgg atcgtaccgg tgatccggat gtcatccagc
     6781 caccagccgt cgtcctgctg cgtcgcggcc cacgtgccgc cgatctcgaa gtagttcgag
     6841 ctgttctcgt cgaacgtcca ggactcggcg atccagcgca gacggacgcg ctgtccgacg
     6901 tacccggaga ggttgaactt ggtctgcacc cagacgccga cgccggtcgc gtcgacttcg
     6961 cccggaccgg cgcagtccac ggtcgccgtg gcgacggtgc cgatggtcga accgcagtgc
     7021 gaccacgtcc ccagcggata gcaggtggtc tcgtgcacgc cccgcgggtt cggcggagcg
     7081 gtgccggtgt cggtcggcgt gaactcgcag tagtagccgc cgaagaccga gaacgcgttc
     7141 ggcttgtggt cgtagacgtt ctggaacggc accagcttgt cccagaagcc ccagttgtcg
     7201 acgttcgcgc ccccgtcgcc gtccaactgg atctggacgt cgccgcagtc cacgcactgg
     7261 cgctcgttgt tcggtccgac gccgttgtta tccatgagtc gggcgatgtg gtagaacgac
     7321 agctccatgt cgcccggccg cgggaacagg gccaggttca tcggccccga ctggtaaccc
     7381 tggagcgtcc ggaagtgggt ggtgtcgccg gccaggttgt cggccgggtc gaagtgcgcc
     7441 cccatgtgca gcgagttgct gccgctgtgg gacttctgtc cgcccaccgg cgtggcgtac
     7501 gagcagccgc cgacgcaggt gccggtctcg aggttcgggc agttcacggc acccggcgag
     7561 cagtgcacgt gccagtccat cgggaagtcc ggatccagct ggcagtaggt gttcgcaccg
     7621 ccgtaggtgt cgtacccgcc gcaggaggtc gccgcgacgg tcccgaccgc gacgccggtg
     7681 gcatcgccgc ggatgtagct gcccatgtag cacagcgccg gagcgccgcc acagtcgtta
     7741 tcggtctggc aggtggtgtt cggggcgttg ctgcagtagc cgcggaaggt gccgggcgcg
     7801 gtggcctgca ggaacgtgtc cttcaccgtg atctggccgt cgccgttctt gtccacgtcg
     7861 aagttctcga cgagggtccc gtcgtcggcc gtgccgttga gaccgtcgtt gcccagcgtg
     7921 aaggctggct gcggcccgcc gggcgggtcg aggtcggcca gcaggctgaa gcagaacgga
     7981 gaggcggtcc ccagcgtctc gttggccacc acggtgatgc agaagttggt cgtcggaatc
     8041 ggcgccggcg gccccgtgta gttgaggccg gcgctggcga tgaactggaa gcccgggttg
     8101 ttgttgacga agtcgccgag cacgcgggtg accccgttct gccactgtcc aagcgcctgc
     8161 ggcggagtgt tgccggggtc ggccgggacc agcaacgtcg gcttggtgat gcaggccacg
     8221 tcactgtcgg acgaggtcag caccaccgtc gcgtcggtca ggttcggacc gaggttccgc
     8281 agggtcaggc ggatgcggcc gttctcaccg gtgtccggga aggcgtcgat gtcgccgtcc
     8341 gtgtcccggt aatcggccgc gaggatcgcc aggttcgcca gcggctgggt gcggcagacg
     8401 atgttgccgt tgaaggccac gacgtcgtcc ggcagaccgt cctgggcgtc atcgaaggtg
     8461 ccggccgggt cgcaggcgtc gccgatgccg tcgcgatcgc tgtcgcgctg gcggtcggtg
     8521 ccggcgatga tggccgcgtt gaagaccgtg gcgcagctgt cacccaggtc cccgatgttg
     8581 tcggcgtcga agtcgtcatc gacggtcgag caggaaccgg agttctggac gtagaagccg
     8641 cagacgttgc cgaggccgaa ggcgttggcg caggccgcct ggttcgattc cggaaccgga
     8701 caggcgacgc cgttggcgcg gcagcggaag acttcgaccg cgttggcgca gtcgccgtcg
     8761 cagacgttgc cgagcccgtc accatcggag tcggtctgga tgttcggacc ctgcagcgga
     8821 ccgttgtcgg cgatggtgca gttgtcgagc gcatcgacga tcccgtcccc gtcgcggtcg
     8881 aggtcgttgt agcagtcggc atgcgtcgcg cacgcctggc cgatcttcgg ggtcggggag
     8941 acgaagttgt tgaagcagac cccgacgttg caggctccac cggcgccgca ggtggcggcg
     9001 acggcgcagg gcgcccccgg agtcgggccg ccgacgcaaa tgccgcagtc cgtgccgcca
     9061 ggcgcgcagg cgttgccgtc gttcggtccg ccgtaacact gcgctccgcc cgccgaccca
     9121 ttgttgccct gggtgcagat gttggtgcgg cacgaccgga tgcagtcctc gtcggtgacg
     9181 cacgcgtcgc cgcgggcatc gcccggcacg gtgcacaggc cggacacgca gtcggccgcg
     9241 ccggcgctgg tgccgaacgt gactccctgc gtcttcagcg tgtcgcacag gtcgccgcga
     9301 ccgtccgctt cgatgtcggc ctggttcggg ttgcggttgt ccgggcagtt gtcggcgtca
     9361 ttcggcagac cgtcgccgtc gacgtcatcg aactcgcagg cgtcgccgat gccgtcggca
     9421 ttggcgtcac cctgatcggc gttgtcgact gtggggcagt tgtcgcagaa ggtgccgaag
     9481 ccgtcggcgt cagtatcctc ctgcagcggg ttgtagagct gcgggcagtt gtcgctggcg
     9541 tccgcaaccc cgtctccgtc gacgttgttg aagttgtcct cgcccgcctg accgtcgaag
     9601 tcaccgtcgc agatcgggtc gtggtagtag acgctgaaga cgccgtccga gccggggttc
     9661 gtgtacgcgt ggttgggatc gttcgtcgtg gtcgagaaga tgatcgtccc acggaacagc
     9721 ccggcgttcc ccgggcccgg gacggcgttc agggtgtaca gcttggcgtt cgggaagcgg
     9781 acctggccgc ccgccgcggc caccggcacg gagtcggtgt cggtgaccac ctggacctgg
     9841 acgctcggga gggtggcgct gcagagtccc cccgctccac acggcgtgtt ggtcgtgcac
     9901 gcgcctccca ccggcaccgt gttgccggcg ccgatggtgc ggcacttcgc gtcgtagacc
     9961 gtgatctcca tcccctcgtc gcactcatag aggctggtcc ggtcggcggt cagcgtggcg
    10021 cactgtccgc cctgtgggaa gttgaggctt gcgttgttga atcgggtaca ggccggctgg
    10081 gctccgagag ccgcctcgtc ctgcgggtgg tactccttcc actcgaagac ggcgtcgtcc
    10141 atcgacttgc cgtagtcggt gtctccggcc gtcccctcca tcgcccagaa accgacgccg
    10201 atctgccagc ggttgctggc gccgcccggg aagaagaagt agaagttctc ctggggaccg
    10261 ttgtcgatca ggctggcgaa gccgccctca tagccgatca gggtggcgtc ccagttgcgg
    10321 accgggccgt tcaccgtgtt gaccgccgcg gtgcagacgc cgccaccgac gatgcacggg
    10381 tcggcgccgt tgcccgggtc gcaggtgtcg tcgttctggg tgccgccgtc gcagatgccg
    10441 atgaccggag cgttcggaac cgggtaggcc aggaagtccg ggatggtggt cggaatcggc
    10501 gtggagctgt cggcgttggt gtcctgggtg tgaccgctga agccggtctc gtcgccgtcc
    10561 aggttcaccg acgcgttcgg gttggtgaac ggcccgaagg tgcgctggaa cggtgcggtc
    10621 gtcgccgggt cgatcccgtc gccggggaag tacgaaccgg cgtcgcggaa catcccgtac
    10681 ggccagccgc cggtccggcg ggtgtagtac gagtccatct gctggcccag caggctgttg
    10741 gcgttgtcgc tgtcgatgtc gttgtcgatg ttgacaccgc cgcccgcgta gccgtcgttg
    10801 agctggatgt tctcgttgta ggcgaagcgc tggaactcca ccgtgaaggc gaagccgcgc
    10861 gcgtcgttgt tctggttcac cttggcgacg attggagact ccaggacgtc catcaggagc
    10921 tcgaccttct caggggtgtt cgggtcgttc ggctgcgtgt gattgtcgca ggccgtcgcg
    10981 gcgccgcccg gggtggtcgg atcgccgtca ccggtgtgcc agatcccgaa cttgttcagc
    11041 ccgccggtgc tctggaagcc gcacagcccg gaggtcttgt actcccacag cggattgacg
    11101 ttgacccccg ccgcgttgct gcccgggtga cggaacggga tccacccgcc attgttgttg
    11161 tcgaagttcc acggcacgcg gtgcagcggg tcggccgggt tgttggagtt caggaccccc
    11221 ttgttgagga tcgcgacccc gccgccgtcc acgtccggca ccacgttgcg ctccacgccc
    11281 gagaagatgc cgttgttgtc gaggtcctct ccgagctggt tggtgaactg gcccaccggg
    11341 gcaccggtgc cgctgaacag gctcgagaac gtcacgtcct cgcgcgtcac gatgaagccc
    11401 aggaacgggt cggtcgtgtc gggcggatcg accacgaggt tgcggttgat gtcgcgcacc
    11461 tggcggccgc cgttcgggaa gtcggtcgag tagaagaatt cctggcggtc cgagttgatc
    11521 gcgtgcttga aggtgtaggt ctggcgcgag atccgggtgc cgcggacgag cgagtcgaga
    11581 ctcagggtca tgtcgacgac gcggttcgcc acggcgaggc cgttggcgat cgtcggatcg
    11641 acgaagacgt ggaagaagac gccgttgacg ccgttcccgg ggaacgaccc catgttcttc
    11701 ggggagtcga ggacgcgaat ggcggccgcg ccgggcccgg agggagtcag ggtggcgacc
    11761 aggtccgcgt agttgttgac ccgggaacgg ttctgcagcg cgatgccgta agtcacgacc
    11821 tcgcccgcat ccaggttgtc gtcgttgtcg cagccgccgg cgacggcgaa ctggttgccg
    11881 agcgcgttcc ccgcgaacgc ggtgaaagcg ccctggatca ggtcgggcga gcaggacacc
    11941 agcgccttcg ccgtgacggc cgcctggccg aggcgggcgt acgtggcgga gatcagcgag
    12001 ccggtatcgg cctcgaggat cccgttgttg gcgaccgccg ggccgccgag acggaccggg
    12061 atcgcggccg aggtgaacga gccggtcgcc gcacccgccg cgtaagtgaa ggacgattcg
    12121 ctgtcggtcg ttgcaccagc ggcgttctgc accaggtagc tcgtgctgga ctggacgttc
    12181 gccggggtcg ccgtgccggt cgtgtcaagc accagcaggc gggcgttcga cgagcagccg
    12241 taccggttcg cgtcccagga gaccatcgac gacgggaaga tggacggacc ggccggcggg
    12301 gccgcctcgg cgaggaacac cgggccggag accaccaggg agtacggctg gcccagctgg
    12361 tcgagcaggc cgttggcgtt gttgtcctcg cccgcgtcca gccgaccgtt gttattgtcg
    12421 tcctcgttct ggtcggggtc ctgcccgacc gtcggggcgg tgatggtgat ctgacccgca
    12481 atcgcgccgc cgaggccgcg cttcacggtc acgcgccaca ggccggtgta gaggggcgac
    12541 tcgtcgaaca ggaagtcgat gttcgtgtcg gcgctcaggt gcaccgcctc ggtgttgttc
    12601 cgcttgtcgt gcttctccac cggggtggtg gaggtgcggc cgagcgacca ctggtcgaag
    12661 ttcgcgttgt tgcggccgcc gtcgtaacgg ttcccgtcgt agaagcggtt gtccgcggcc
    12721 gtcgcgaggt tgccgtccgg accggggctc tcgaccacca ggtcgaggtc gttgaccagc
    12781 ggaccgccgg tgccggcggc gctgggcgga tccgcccacg aca
//
LOCUS       DASRIQ010000267         3913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1676743, whole genome shotgun sequence.
ACCESSION   DASRIQ010000267 DASRIQ010000000
VERSION     DASRIQ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676743"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3913
                     /locus_tag="VFD06_06585"
     CDS             <1..>3913
                     /locus_tag="VFD06_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03239.1"
                     /translation="GLRFTATGDNGNVGTATTFDVRYRIGAPINNEADFAAAIAAAGE
                     PAPGPAGTVTFYAIVPLAASTPYFIRMKVLDEVPNASTLSNQVTATTTAVDGAAPASV
                     TLAGTPNGLGLVGLTWTAPGDDGPFGIAAGYDLRRSLTPITTLAAFNAATPVAGEPPP
                     APAGTPQGMTVGGLAPGTDWSFAVRAFDEAGNGSTVPAMAMTVATLPQFLEFGPVTNL
                     NRIETIATSYSAVTSLGGGSWRLSGNADVTLGGALRSFAFANLDVATTGELANDGVSR
                     AARVTAGSVTVNIAGNPIDYPLAGDLDLQLLSFRLSSIGGEIVPGGLRLTLPPGLTSS
                     IGDVLTFFVTTGLAQDLDFNAIVTPGPGFFFAPKDYPFRIAPEVGILYIATRSRVELG
                     PSLYSPIDKERGILSPAGCAVAGACIEQANDLYFSFPWTFNAYAGGGGPPGTIGTPYF
                     EPDGLRATLVLTADGTHRPLFPAGVEIPILAGSRIEFIAGEPAGGILAGEVELTMSRG
                     ARVPDGEGGIRADCSSSSDLVRSAGFAAGEIQVGGRMTIDAMTLIPGKVFSEIPWGQP
                     GVNPGDPQTSGFFVGGLDSSNLSFYAPGTVARLTTARPSVQAHLLAERDDLSGAGTYA
                     GMNIAMDAGAPGTFGLDSLCPEAPDVPGGHSLEVSSDSTRLYARASGVSGIGDGGSLD
                     PPRDFDYMGYDMTLSHFAVAFLDNAVPTREGSGVVISHVQIPNPSGIGFDFESPAGID
                     SCGGFTDSLGDMTNAGTPQTLVCWEADFTPFAARFQKVDAELEPECAGVAPPPRCCIG
                     ETPPLCPDLDASEVRYLHFSARVPFELEPGTPAVAHFDEQVPMDFAPGPLGNLVCNDI
                     QPTTPGGTVRNEFEPEYGFDVDVTRAALRPENPACPAPLKGGPAPAVPDPDPARYEVE
                     GETLFPFYGGTAGCAVVKQLGLVEVGGSCLPNAPPPMHVHRRVFADLFALDFDLHYLP
                     PIRLPDARATAARFFAQDATLDAGLFTVPVAVKLLGEVSDGAPGVPQTEEAPQLFFGF
                     LSDLSAYVEAIQDNDVCDAACLDRLEDLVDLDVGNPEIDVDRLLPGMRAYYDRAKALG
                     VPDLRRVAADALHQTVGSVLPADFVSGARRARGVVSNLMAVTDAFKTKDLTGAGVFET
                     YAGEAADYVLDTAQLDTEVDALAFLNFKGFVHFNRHTANAVGDTDDTAHLTIGAHDVD
                     LSWAIDSVRARTIEGTFLFDPGPTFTGFEGMIELTGMRFGEVAVDQAGLLLGLGNPAG
                     PDNDFHYVAASARGGYKNVTAEVGLFLGKSIDLDPLIYVDPD"
BASE COUNT          547 a         1448 c         1327 g          591 t
ORIGIN      
        1 cggcctgcgc ttcacggcga ccggcgacaa cggcaacgtc ggcacggcga cgacgttcga
       61 cgtgcgctac cggatcggcg cgccgatcaa caacgaggcc gacttcgcgg cagcgatcgc
      121 cgccgccgga gagccggcgc cggggcccgc cggcaccgtc accttctacg cgatcgtccc
      181 gctggcggcc tccaccccgt atttcatcag gatgaaggtg ctggacgagg tgccgaacgc
      241 ctcgaccctg tcgaaccagg tgacggccac gacgaccgcc gtcgatgggg cggcgcccgc
      301 ctcggtgacg ctcgcgggga ccccgaacgg gctgggtctg gtcgggctga cgtggacggc
      361 tccgggggac gacgggccgt tcgggatcgc ggccggatac gacctgcgac gctccctcac
      421 gccgatcacc accctggcgg cgttcaacgc ggcgacgccg gtcgcggggg agccgccgcc
      481 ggcgccggcg ggcacaccgc aggggatgac ggtcggcggg ctggcccccg ggaccgactg
      541 gtctttcgcc gtgcgcgcct tcgacgaggc ggggaacgga tcgacggtcc cggcgatggc
      601 catgaccgtg gcgacgctgc cgcagttcct cgagttcggt ccggtgacga acctgaaccg
      661 gatcgagacg atcgccacct cctattcggc cgtcaccagt ctcggtgggg ggagctggcg
      721 gctgtcgggg aacgccgacg tcacgctcgg cggggcgctg cgctccttcg cgttcgccaa
      781 cctcgacgtt gccacgaccg gggagctggc caacgacggc gtcagccgcg cggcgcgggt
      841 cacggccggg tcggtcacgg tgaacatcgc cggcaatccc atcgactacc cgctcgccgg
      901 cgatctcgac ctgcagctcc tgtcgttccg gctgagcagc atcggcggag agatcgtccc
      961 gggcggtctg cgcctgacgc tcccgcccgg gctgacctcc tcgatcggcg acgtcctcac
     1021 gttcttcgtc accaccggtc tcgcccagga cctcgacttc aacgccatcg tcaccccggg
     1081 gcccggattc ttcttcgcgc cgaaggacta cccgttccgg atcgccccgg aagtcgggat
     1141 cctgtacatc gcgacccgat cgcgcgtcga gctcgggccg tccctctaca gcccgatcga
     1201 caaagagcgc ggcatcctca gcccggccgg ctgcgccgtc gccggcgcct gcatcgagca
     1261 ggccaacgat ctctacttct ccttcccctg gaccttcaac gcctatgccg gcgggggcgg
     1321 gcctccggga acgatcggca caccgtattt cgagccggac ggcctgcggg cgacgctcgt
     1381 cctcaccgcc gacgggacgc accggccgct cttccctgcc ggggttgaga tcccgatcct
     1441 cgccggatcg cggatcgagt tcatcgccgg ggaaccggcg ggcgggatcc tcgcggggga
     1501 ggtcgagctg accatgtcgc gcggcgcccg cgtgccggat ggcgagggag gaatccgcgc
     1561 cgactgctcc tcgtcgagcg acctcgtccg ctccgccggc ttcgccgccg gagagatcca
     1621 ggttggcggc aggatgacga tcgatgcgat gacgctgatt ccgggcaagg tcttcagcga
     1681 gatcccctgg ggccagccag gggtcaatcc cggcgatccg cagaccagcg ggttcttcgt
     1741 cggcggtctc gattcctcga atctttcctt ctacgcgccc ggcacggtgg cccgcctgac
     1801 caccgcccgt ccctcggtcc aggcgcacct gctcgccgag cgggacgacc tgtccggcgc
     1861 cgggacctac gccgggatga acatcgccat ggacgccggc gccccgggga cgttcggact
     1921 cgactccctc tgccccgagg cgccggacgt ccccggcggt cattccctcg aagtctcgtc
     1981 ggattccacg cgcctgtacg cccgcgcctc gggggtgagc ggcatcggcg acggcggttc
     2041 cctcgatccg ccgcgcgact tcgactacat ggggtacgac atgacgctgt cccacttcgc
     2101 ggtggcgttc ctggataacg ccgtcccgac gcgggagggc agcggcgtgg tcatcagcca
     2161 cgtccagatc ccgaacccct ccggcatcgg cttcgatttc gagagcccgg ccggcatcga
     2221 ctcgtgcggc gggttcaccg acagcctcgg cgacatgacc aacgccggca cgccgcagac
     2281 cctggtctgc tgggaagccg acttcacgcc gttcgccgcc cgcttccaga aggtggacgc
     2341 ggagctggag ccggagtgcg ccggggtcgc gccgccgccg cgctgctgca tcggcgagac
     2401 gccgccgctg tgcccggacc tggacgcctc cgaggtgcgc tatctccact tcagcgcccg
     2461 cgtcccgttc gagctggaac cggggacgcc ggcggtcgcc cacttcgacg agcaggtgcc
     2521 gatggacttc gccccgggac cgctcggcaa tctggtgtgc aacgacatcc agccgaccac
     2581 cccgggagga acggtgcgca acgaattcga gccggagtac ggcttcgacg tggacgtcac
     2641 ccgggccgcg ctgcggcccg aaaacccggc ctgcccggcg ccgctcaagg gggggcccgc
     2701 gcccgccgtc cccgatcccg acccggcccg ctacgaggtg gaaggggaga ctctgttccc
     2761 gttctacggc ggcaccgccg ggtgcgccgt cgtcaagcag ctcgggctcg tcgaggtcgg
     2821 cgggtcgtgc ctgccgaacg ccccgccgcc gatgcacgtc caccggcgcg tcttcgccga
     2881 cctgttcgcc ctggacttcg atctgcacta cctgccgccg atccgcctcc cggacgcgcg
     2941 cgccaccgcc gcccgcttct tcgcgcagga cgcgacgctc gacgccggtc tgttcaccgt
     3001 gccggtggcg gtcaagttgc tcggggaagt cagcgatggc gcgccggggg tgccgcagac
     3061 ggaggaggcg ccgcagctgt ttttcgggtt cctgtcggat ctctccgcct acgtcgaggc
     3121 gatccaggac aacgacgtct gcgacgccgc ctgcctcgac cgcctggagg acctcgtcga
     3181 tctcgacgtg gggaacccgg agatcgacgt cgaccggctc cttcccggca tgcgggccta
     3241 ttacgaccgg gcgaaggcgc tcggcgtccc ggacctgcgg cgggtcgccg ccgacgcgct
     3301 gcaccagacg gtcggcagcg tcctgccggc cgacttcgtc agcggcgcgc gccgggcgcg
     3361 cggggtcgtc tccaacctga tggccgtcac ggatgccttc aagacgaagg acctcaccgg
     3421 ggccggcgtg ttcgagacct acgcggggga ggcggccgac tacgtcctcg acacggcgca
     3481 gctcgacacc gaggtggacg ccctggcgtt cctcaacttc aaggggttcg tccacttcaa
     3541 ccgccacacg gcgaacgccg tcggcgacac cgacgacacc gcccatctga ccatcggggc
     3601 gcacgacgtc gatctctcct gggcgatcga ctcggtgcgg gcacggacga tcgaggggac
     3661 cttcctgttc gatccggggc ccactttcac cggcttcgag gggatgatcg agctgaccgg
     3721 gatgaggttc ggcgaggtgg cggtcgatca ggccgggctc cttctcggac tcggcaatcc
     3781 cgccgggccg gacaacgact tccactatgt cgccgcctcg gcccgcggcg gctacaagaa
     3841 cgtcacggcg gaggtcggcc tcttcctcgg caagtcgatc gatctcgatc cgctcatcta
     3901 cgtcgacccc gac
//
LOCUS       DASRIQ010000268         4879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_363252, whole genome shotgun sequence.
ACCESSION   DASRIQ010000268 DASRIQ010000000
VERSION     DASRIQ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363252"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1238
                     /locus_tag="VFD06_06590"
     CDS             78..1238
                     /locus_tag="VFD06_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HZN03240.1"
                     /translation="MPARPSFTIGIEEEFQVIDPKTRELRSHIAEIFEQGKAVLKERM
                     KPELHQSVVEIGTEICRDIQQAQRDVTATRTHIARLARDNGLRIAAAGTHPFSHWATV
                     EITGGNPRYDRLIEELQMVARANLIFGLHVHIGVEDREAQIRIMNQARYFLPHVLALA
                     VNSPFWLGRDTGWMSYRCKVFDKFPRTNIPDFFGSYGEFQQYVDLLVKTGCVIDGRQI
                     WWDVRPHVLYPTLEYRICDIPMRVEETIAIAALFQAITAKLWRLGSGNLTFRPYRRSL
                     IMENKSRAARWGLNGRLIDFGKEEEVDVKSLIAELMEFVDDVVDDLGSRDALRYVGTI
                     LEHGTGADRQLEVYRKTGDLKAVMDYVVSETERGLDLGPMTEPPRRAPEARL"
     gene            1235..2287
                     /locus_tag="VFD06_06595"
     CDS             1235..2287
                     /locus_tag="VFD06_06595"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZN03241.1"
                     /translation="MKGVMVARDLRTVPYDPEFRPGAHSAVFTCLRVTPGERVVLITD
                     EEALGIGASLFEQFEKAGAKVEAFVLEDFAPRPLGEFPPVIADAMRTSQVSCFAASAQ
                     PGELRIRIAMTAVANEKRLRHAHMVNITPRIMVEGMRADFARVDALSRWVFERASRAK
                     TLTASTPAGTRLTATFDPRLRWLKTSGIITPEKWSNLPGGEVLTAPARVDGVYVVDGV
                     LGDWLAPKYGDLSGTPMAVHIEDSRIAKVECARRDMVDDFLAYTSADPNGNRVGELAL
                     GTNLAVKDVIGQILQDEKIPGLHIAFGHPYSEHTGADWRASTHIDIVGRHFDVAFDGV
                     PIMRNSRYLVDAASLA"
     gene            2325..3548
                     /locus_tag="VFD06_06600"
     CDS             2325..3548
                     /locus_tag="VFD06_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03242.1"
                     /translation="MDPRLRAIFNRNFSDGLEARMQALMDERLATPRFAFRLAETPVF
                     LTPDLRDRCARTAREILEQISKPEVIGACQAAIPERYRTPRPDPLPHFLAIDLALVRG
                     ASGEIEPRLIELQGFSSLYGMTFVQSRVWSELLAEMPGMPQAFTALFSGLDGDGYLDL
                     LRRTVVADAAPEEVILLDLDPETQKTVPDFRAAARFLGVRSVCVSELRREGRRLFAPV
                     PDGRMVPVRRILHRVVFDELERRPVPMCFDYRDDLDVVWAAHPIWYWVWSKYTLPLLR
                     HPAAPRTWRLSEVERLPDDLQNYILKPIFSFAGRGVVVDLDRATIDALPGEERSNYLL
                     QEKVEYAPALTAPDGSGVKAEIRMMFLRPEGAYDMTLAINLARLSRGKMHGVDHNKGL
                     RWVGSSVALWPAASA"
     gene            complement(3589..3996)
                     /locus_tag="VFD06_06605"
     CDS             complement(3589..3996)
                     /locus_tag="VFD06_06605"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN03243.1"
                     /translation="MSDEKKDPAATELDPSLAPRRMKSGLVPPDTGLYLRIEEGPGQG
                     RTFVLSAGGVYTIGREGADIALEDGKVSRKHAEIGLYGPDAWILRDLASTNGTSLNGR
                     RIGERINLKHWDLIRVGDTRLRFAVIEKSIQVA"
     gene            complement(4039..>4879)
                     /locus_tag="VFD06_06610"
     CDS             complement(4039..>4879)
                     /locus_tag="VFD06_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03244.1"
                     /translation="HETPAPVRHRVPDLPPGLDAFFARAFEKDPADRYQDAAAFRAAL
                     LEAGRADAIDAAPPGSIEEESPDESAPEEPKPKRRRGSKRKEKPRPQPAAIDTTADAS
                     EPAVIPAVMDGDAGAGYSQAQGLRAVWVPLLIAVLFFVTLGGAPFVFARRPAYVRIET
                     RSAEPGSLRVLLDGKDVATLPQIGPALASVSFRPASPGRAAAVAGAPPDGASAFALVA
                     TFDVWLQAPRGRHTLDVVARLPGLAEPARAKLEVDLTPGETRTVRLNAVTGPDGLPHL
                     RLD"
BASE COUNT          695 a         1756 c         1714 g          714 t
ORIGIN      
        1 ggcgcagcag cccggcgagc cgcgggcccc gcggccggcc ggccggtgag cctcccggcc
       61 gcccggcgga ggagctgatg ccggcccgcc cctccttcac catcgggatc gaggaggagt
      121 tccaggtcat cgatccgaag acgcgcgagc tgcgctccca catcgccgag atcttcgagc
      181 aggggaaggc ggtcctcaag gagcgcatga agccggagct gcatcagtcg gtcgtggaga
      241 tcggcaccga gatctgccgc gacatccagc aggcgcagcg cgacgtcacg gcgacccgca
      301 cccacatcgc ccgcctggcc cgcgacaacg gcctgcgcat cgccgccgcc ggcacgcatc
      361 ccttctccca ctgggcgacg gtcgagatca ccggcgggaa tccccgctac gaccggctca
      421 tcgaggagct gcagatggtg gcgcgggcca acctcatctt cggcctgcac gtccacatcg
      481 gcgtcgagga tcgcgaggcc cagatccgga tcatgaacca ggcgcgctac ttcctgccgc
      541 acgtcctggc gctggcggtg aactcgccgt tctggctcgg gcgcgacacc ggctggatgt
      601 cttaccgctg caaggtgttc gacaagttcc cgcgcaccaa catcccggac ttcttcggct
      661 cgtacggcga gttccagcag tacgtcgacc tgctcgtcaa gaccggctgc gtcatcgacg
      721 ggcggcagat ctggtgggat gtgcggccgc acgtcctcta tcccaccctc gagtaccgca
      781 tctgcgacat cccgatgcgg gtcgaggaga cgatcgccat cgccgcgctg ttccaggcga
      841 tcaccgccaa gctgtggcgg ctggggagcg gcaacctgac gttccgcccg taccgtcgct
      901 ccctgatcat ggagaacaag tcgcgcgccg cccgctgggg cctgaacggg cggctcatcg
      961 acttcggcaa agaggaggaa gtggacgtga agagcctcat cgcggaactc atggagttcg
     1021 tcgacgacgt ggtcgacgac ctcggctcgc gcgacgcgct gcgctacgtc gggaccatcc
     1081 tcgagcacgg caccggcgcc gaccggcagc tggaggtgta ccggaagacc ggcgacctca
     1141 aagcggtcat ggactacgtc gtctcggaga ccgaacgcgg cctcgacctc ggcccgatga
     1201 cggagccgcc gcggcgcgcc cccgaggcgc gactgtgaag ggggtcatgg tggcgcgcga
     1261 tctgcggacc gttccctacg acccggagtt ccggccgggg gcgcacagcg cggtcttcac
     1321 ctgcctgcgg gtgacccccg gggagcgggt cgtgctgatc accgacgagg aggcgctcgg
     1381 gatcggcgcc tccctcttcg agcagttcga gaaggcgggg gcgaaagtcg aggcgtttgt
     1441 gctggaggac ttcgcgccgc gccctctcgg ggaattcccg ccggtgatcg ccgacgcgat
     1501 gcggacgtcc caggtgtcct gcttcgcggc ctcggcgcag ccgggtgaat tgagaatccg
     1561 catcgccatg acggccgtcg ccaacgagaa gcggctgcgg cacgcccaca tggtcaacat
     1621 cacgccgcgc atcatggtgg aggggatgcg ggccgacttc gcgcgggtgg acgcgctctc
     1681 ccgctgggtg ttcgagcggg cgagccgggc gaagaccctc accgcctcga ccccggccgg
     1741 cacgcgcctg accgccacct tcgatccgcg cctgcgctgg ctgaagacct ccggcatcat
     1801 cacaccggag aagtggtcga acctcccagg cggcgaagtg ctgaccgcgc cggcgcgtgt
     1861 ggacggcgtc tacgtcgtgg acggggtcct cggcgactgg ctggcgccga aatacggcga
     1921 tctctccggc acgccgatgg cggtgcacat cgaggactcg cgcatcgcga aggtggagtg
     1981 cgcccgccgc gacatggtgg acgacttcct cgcctacacc tcggccgatc cgaacggcaa
     2041 ccgcgtgggg gagctggcgc tcggcaccaa cctcgcggtc aaggacgtca tcggacagat
     2101 cctccaggac gagaagatcc cggggctgca catcgccttc ggccacccgt acagcgagca
     2161 caccggcgcc gactggcgcg cctcgaccca catcgacatc gtcggacggc acttcgacgt
     2221 cgcgttcgac ggcgtgccga tcatgcgcaa cagccggtac ctcgtggacg ctgcctcgct
     2281 cgcctgagcg ggcctccgtc cgaatctccc ggggacgtca cgccttggac ccgcggctcc
     2341 gcgccatctt caaccggaac ttctcggacg ggctcgaggc gcgcatgcag gcgctcatgg
     2401 acgagcggct ggcgacgccg cgcttcgcct tccgtctcgc ggagacgccg gtcttcctga
     2461 cgccggacct gcgcgatcgc tgcgcgcgaa cggcccgcga gatcctcgag cagatctcga
     2521 agccggaggt gatcggcgcc tgccaggcgg cgatcccgga gcgctaccgc acgccgcgcc
     2581 ccgacccgct gccgcacttc ctcgccatcg atctggcgct ggtgcgcggc gcgtcggggg
     2641 agatcgagcc gcggctcatc gagctccagg ggttctcgtc gctctacggg atgacgttcg
     2701 tacagagccg cgtctggtcg gagctgctcg cggagatgcc ggggatgccg caggcgttca
     2761 ccgcgctctt ctccgggctc gacggcgacg gttacctcga tctcctgcgc cgcacggtcg
     2821 tcgccgacgc ggcgccggaa gaggtgatcc tcctcgacct cgatccggag acgcagaaga
     2881 cggtccccga cttccgcgcc gcggcgcggt tcctcggcgt gcgctcggtc tgcgtctcgg
     2941 agctgcggcg cgaggggagg aggctcttcg ccccggtgcc ggacgggcgg atggtcccgg
     3001 tgcgccgcat cctgcaccgg gtggtcttcg acgagctgga gcgccgcccg gtgccgatgt
     3061 gcttcgacta tcgcgacgat ctcgatgtcg tctgggcggc gcatccgatc tggtactggg
     3121 tctggtcgaa gtacacgctg ccgctcctgc gccatccggc cgccccgcgc acctggcgcc
     3181 tgtcggaggt ggagcggctg ccggacgacc tccagaacta catcctcaag ccgatcttct
     3241 cgttcgcggg gcgcggggtc gtggtcgatc tcgaccgcgc gacgatcgac gccctccccg
     3301 gggaggagcg ctccaactac ctccttcagg agaaggtgga gtacgcgccg gcgctgaccg
     3361 cgccggacgg ctcgggcgtg aaggcggaga tccggatgat gttcctgcgg ccggaggggg
     3421 cgtacgacat gacgctcgcc atcaacctcg cccgcctgtc gcgcggcaag atgcacggcg
     3481 tcgaccacaa caaggggctg cgctgggtcg gatcgtcggt cgccctgtgg ccggcggcat
     3541 ccgcctgacg cgacgccggc cggcgtccac ccgcccgatg cgacgcgatc aggcgacctg
     3601 gatcgacttc tcgatcaccg cgaagcgcag ccgggtgtcg ccgacccgga tcagatccca
     3661 gtgcttcaga ttgatgcgct cgccgatgcg ccggccgttc agcgacgtgc cgttggtgct
     3721 ggcgagatcg cggaggatcc acgcgtccgg gccgtacagc ccgatctcgg cgtgcttgcg
     3781 cgacaccttg ccgtcctcga gggcgatgtc ggccccctcg cggccgatgg tgtagacgcc
     3841 gccggccgac agcacgaacg tccgcccctg tcccggcccc tcctcgatgc gcaggtacag
     3901 gccggtgtcg ggggggacca gcccggactt catgcggcgc ggcgccaggc tcgggtcgag
     3961 ctcggtcgcc gccgggtcct tcttctcgtc gctcatgtcg gcctcctccc gtcccgcgcc
     4021 ccggcgcggg acgcggcgtc aatcgagtct caggtgcggc agtccgtccg gtccggtcac
     4081 ggcgttgagg cgcaccgtgc gcgtctcccc cggagtgaga tcgacctcga gcttcgcccg
     4141 cgccggctcc gcgaggcccg gcaggcgcgc cacgacgtcg agcgtgtggc ggccgcgcgg
     4201 cgcctgcagc cagacgtcga acgtcgcaac cagggcgaaa gccgacgcgc cgtccggcgg
     4261 ggcgcccgcg acggcggcgg cccggcccgg ggacgcgggc cggaacgaca cggaggcgag
     4321 cgcggggccg atctgcggca gggtcgcgac atcctttccg tcgaggagga cgcgaaggct
     4381 tcccggctcc gcgctgcgcg tctcgatccg cacgtaggcc ggccggcgcg cgaagacgaa
     4441 cggcgccccc ccgagcgtca cgaagaagag gacagcgatc aggagcggca cccacacggc
     4501 gcgcagacct tgcgcctggc tgtaccccgc cccggcgtct ccgtccatga cggccgggat
     4561 gacggccggc tccgaggcat cggccgtggt gtcgatcgcg gcgggctgcg ggcgcggctt
     4621 ctccttgcgc ttcgagccgc gccggcgctt cggcttcggc tcctctggcg cgctctcgtc
     4681 cggagactcc tcctcgatcg agcccggggg cgccgcgtcg atcgcgtcgg cgcgccccgc
     4741 ctcgagcagg gcggcccgga acgccgcggc gtcctggtag cgatccgccg gatccttctc
     4801 gaaggcgcgc gcgaagaacg cgtcgagtcc gggcgggaga tccgggacgc ggtggcgcac
     4861 cggcgccggc gtctcgtgc
//
LOCUS       DASRIQ010000269        10052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1677376, whole genome shotgun sequence.
ACCESSION   DASRIQ010000269 DASRIQ010000000
VERSION     DASRIQ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677376"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1681
                     /locus_tag="VFD06_06615"
     CDS             56..1681
                     /locus_tag="VFD06_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03245.1"
                     /translation="MAIRRFLPAVLLLSCAALAVAAVDRDKDDDRPLDARLYSQGMEA
                     ARAGRRDEAGRLLRRVFTEFPDSPSAPPAILKTVELVYPVTDWSQIGSASPQSVQQAT
                     DLLKVLAQKYRASREAPQALVRLGYVGFEPAGPRLNLDEACGRFATAAQIYPDSAAAD
                     DALFASGMCDLLRSRPARASEMFGRMLEEHPESPLAGEAQYRQGVALSLLDDAGEAML
                     ALQRVRSRGPDSRFTSQALDRLTLLHRMRLQPALAAAAGREGATPPAPEPPSSEGTPL
                     FRPDSDYGPDPRAAGANPPQAIRGITDLSIDSQGLIVAASPKTPAVFRLDGKGRIQER
                     IDHPGPDHVAAAEGLAVFISGREQIAVNQRNWSGTDLRDANGHAPSDFGPIAVDTLGT
                     VHLLDRRENAVLVYDRARRLVGAVRPPAGRDGRFVDIAAGVEGAVYALDARARSVVVL
                     AQGRETKRIDLTPLGAADPTALAVDGLGDLFILDGGTGWVFVGDPQGKRIAVVRPSKI
                     VTDRTGAITAIEVDAQGRLYLAGRRSGAVVRLR"
     gene            1678..3891
                     /locus_tag="VFD06_06620"
     CDS             1678..3891
                     /locus_tag="VFD06_06620"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZN03246.1"
                     /translation="MIRRLAFACGLALLLLAAPLPPAGTARAQEEEDLTKELVAMRAA
                     FDRARERLDELDFTAAVRELGTIIEPRARTRPSDLGFEETRLLASAYDLRARAQFNLG
                     NAAAAEADFGSLLRLDPSYSIDRQTLSPKVVDLFDRVRAKTVGILNLTVDPARARVKV
                     DGDPVDAGPEGIGVLSGTHELRVEMEGYDPHVETITAAGGARLERRVRLRPNRRSLEF
                     ITVPTGVTVMIDDAAAGTSAGPPTPEVEGLAATYGFDARKASAPLLVPTVTPGEHRVS
                     FQRDCYETQSVVVRVELDPEQNRPMRFAPVVLKESRTRLQITSVPAGAEVQVDGVPQG
                     TTPLTLESLCGGDREITVVRREVGRWSERVRLATGQVNTLDVRLRPTLLYLGTFRLDE
                     WGRAVWSDEDKPLLDALGRGLKTLNVARVPEVQQSIRDAVIRWMIADPREARAGTLVP
                     PDLLRDAAERAGADLILAGLTLADDPERSWTLGLYSALHTTPDVVRLRLDRPAGVADF
                     VARLDSAPQQGSTLWGIGLADSALPPGGPVVARVLAGSPAAKAGVRLGDRVTSIGTKK
                     TTSTREALAALASESSRGGGARSAVVVTLQGTDGTRTTRIAPVEAPALLSLTDPQILY
                     NRALVEYRLRARAAADETARGVAALNTGLALMHFRAYDKALAEGLQRASLPPGSGIAQ
                     GTVDYYRGLCAQRRGDTDGARAAFQAAVKAAGSTLESPEGPSASAAATRALQSLQ"
     gene            3965..4567
                     /locus_tag="VFD06_06625"
     CDS             3965..4567
                     /locus_tag="VFD06_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN03247.1"
                     /translation="MRATAFGILLAALSAPAGFAPLFGAENEGALIHGIIYRSDEATR
                     LQGALVTAINVRTGRRYSSIHTGANGAYEIAGLPAGTYDIAIDTPDDNLYVTDGLIDL
                     HENQRLMLSLSLKKSGGAVPGGPARGEPTQSFTDPGAVPPTFAPGAATPLPPAAEASG
                     GKSDKKPEKKPKKPVGTKAKTGSGATATLRLEPASSFPVS"
     gene            4582..5754
                     /locus_tag="VFD06_06630"
     CDS             4582..5754
                     /locus_tag="VFD06_06630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZN03248.1"
                     /translation="MTAETERVAALDPAAEYAAVREEIDAAVSRVLASGRYVGGPEVE
                     GLEAEFAAFCGVTHAVAVSTGTDALRFALIASGLPAGGEVVTSPFTFIGTTEAIHQAG
                     GRAIFADILPDTFAIDPARVKAALTLRTAAVLPVHLYGHPADMDSLQPEAGHRRLTIV
                     EDACQAHGASLHGRMVGGMGTAGCFSFYPTKNLGACGEGGMVTTGDAAIAARVRRLRD
                     HGQSEKYLHAEEGYNGRLDAVQAAILRVKLRRLWEWNERRRSLATLYADRLAPLEGRG
                     WLRRPVERPGVKHAWHLYAVRIPSEAADLRAADRTSRRDQVRARLLGQGIETGIHYPV
                     PLHRQPAYASLGLAEGSFPEAERAAREVLTLPIHPHLSERQAERVATALAAALESA"
     gene            5751..6869
                     /gene="wecB"
                     /locus_tag="VFD06_06635"
     CDS             5751..6869
                     /gene="wecB"
                     /locus_tag="VFD06_06635"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZN03249.1"
                     /translation="MSRRRRARVLLVAGARPNFMKVAPLLRALRGRPDRFEPILVHTG
                     QHYDETMSDVFFKELGIPRPDRHLGVGSGSHAAQTARIMEAFEPVLHDAKPDRVVVVG
                     DVNSTAACALVASKMAPPVPVAHVEAGLRSFDRAMPEEINRLVTDALSDLLFTTSPDA
                     DRNLRSEGVPRRRIHRVGNLMIDTLRAFVRRADGSDVLRRLRLAPPYALLTLHRPSNV
                     DDETTFRRILAALEEIGRGLPVLFPAHPRTTERLRRLGAGNGTRGLRLVEPLGYLDFL
                     HLQKRASLVLTDSGGVQEETTILGVPCLTLRENTERPITLTRGTNRLVGTDTRRIVTE
                     ARRALRKRRGRAPTIPLWDGRAAERIARVLGAERRVED"
     gene            7014..7703
                     /locus_tag="VFD06_06640"
     CDS             7014..7703
                     /locus_tag="VFD06_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZN03250.1"
                     /translation="MIRQRSIAVAIKRALRRERHRIAADLHDELGQAMAAAHYNLSLL
                     EAWAIGGPRDVRRILREVTRIVSRTLGETRRIAADLQPRAIDGKGFEPALRGLLKSFS
                     RRTGARARLQIEGGALPLCLGAQSLLFRILQESLNNVAAHARARNVAIRLVCSDGGTV
                     MSVHDDGIGLRAVGRRAPTSGLGLAGMRRRVRDVGGSLRLHSTPGRGTRLVVTLHRRE
                     PVPGPREMARR"
     gene            7700..8356
                     /locus_tag="VFD06_06645"
     CDS             7700..8356
                     /locus_tag="VFD06_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN03251.1"
                     /translation="MSPNRKIRVLLADDHTLVRQGLRALLEREADIKIIGEARNGREA
                     VNRVLALKPDIVVMDISMPHLNGFEATRRIAAECPETRVVALSLHKEEHHVQALLRAG
                     ARGYVPKDAPASELLAAIRAVDGGGCYLHPQISAKVVDGYLDGNRPPGESEPLTPRER
                     EVLQLVAEGRTNRLIASELRLSIKTVEAHRARIMTKLKIHNAAGLTRYAIGRGIIGSD
                     "
     gene            8566..>10052
                     /locus_tag="VFD06_06650"
     CDS             8566..>10052
                     /locus_tag="VFD06_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN03252.1"
                     /translation="MKNGTRRDLRHPKGSLAPPAQAAGARTRPGGSGAVVLPGREDEE
                     SLKVVEPVAAIPASRRARAKARARRGARRFPVEKVIAESGRWLDIIARGDRLTGAQKK
                     RVIKETVKSFSTYFNTGFLAYRKSVAEAEQYASIEWTGQGTMIEDITGRQYIDCLGGF
                     GIYSAGIRHPKIVEAVRNQLDRMPLSSQELLDPLRGALAELLGEIAPGNLQKCFFINN
                     GTDAVEGAMKLARLYTEKSGFISTIRGFHGKSYGSLSLMGKAEFRIAFEPLLQDVYFV
                     EFGDADEVEWQLKKARDVGADIAAVVAEPVQGEAGAIVPPDDYWPRLRAICDEFDVLL
                     IADEVQTGMGRTGKMFGVDHWDVVPDIMCLGKSLGGGVMPLSAFVSTPEIWKVLEQNP
                     FLHSSTFGGNPLACAAGIAAIHVTLSEDLPGQAAERGAEFIQGLRDIQARYSDVIVDV
                     RGKGLLIGVEFNDTEFGYSVAAGLFRRGVLVAGTLLNAKTIRIEPA"
BASE COUNT         1426 a         3652 c         3609 g         1365 t
ORIGIN      
        1 gcaagaacgt gacgatccgc gccgaccgga ccgagcgcgt cgtggtgaag ttctgatggc
       61 gatccggcgg ttccttccgg ccgtcctgct cctgtcgtgc gccgccctcg ccgtggcggc
      121 ggtggacaga gacaaggacg acgaccggcc gctcgacgcg cggctgtaca gccaggggat
      181 ggaggcggca cgcgccgggc gccgcgacga ggccgggcgg ctgctgcgcc gggtcttcac
      241 cgagttcccg gacagcccgt cggccccgcc ggccatcctc aagaccgtgg agctggtgta
      301 tccggtgacc gactggagcc agatcgggtc cgcctcgccg cagtcggtgc agcaggcgac
      361 cgacctgctc aaggtcctgg cccagaagta ccgcgcctcg cgggaggcgc cgcaggcgct
      421 ggtgcggctc gggtacgtcg gcttcgagcc ggccggcccg cggctgaacc tcgacgaggc
      481 gtgcggccgc ttcgcgacgg cggcgcagat ctatcccgac agcgccgccg ccgacgacgc
      541 gctgttcgcc tccggcatgt gcgatctctt gaggtccagg ccggcgcgcg cctcggagat
      601 gttcggccgg atgctcgagg agcaccccga aagccctctg gcgggggagg cgcagtaccg
      661 gcagggcgtg gccctctccc tcctcgacga cgcgggggag gcgatgctgg cgctgcagcg
      721 ggtgcgttcg cgcggccccg actcccgctt cacctcccag gcgctcgatc gcctgacgct
      781 cctgcaccgg atgcgcctgc agccggcgct cgccgcggcc gcggggcgcg agggggccac
      841 gccccctgcc cccgagcccc cttcgagcga ggggacgccg ctgttccgcc ccgactcgga
      901 ctacggcccc gatccgcgcg ccgccggcgc gaaccccccg caggcgatcc gcggcatcac
      961 cgatctcagc atcgactcgc aggggctcat cgtcgccgcg tcgccgaaga caccggcggt
     1021 cttccggctc gacgggaagg ggcgcatcca ggagcggatc gatcaccccg ggccggacca
     1081 cgtggcggcg gccgaggggc tggcggtctt catctcgggg cgcgagcaga tcgccgtcaa
     1141 ccagaggaac tggagcggca ccgacctgcg cgacgcgaac gggcacgccc cgtccgattt
     1201 cggaccgatc gccgtggata cgctcggcac cgtgcacctc ctcgaccggc gcgagaacgc
     1261 ggtcctggtc tacgaccggg cgcggcggct ggtgggggcg gtgcgtccgc cggccgggcg
     1321 ggatggccgc ttcgtggaca tcgccgccgg cgtcgaaggg gcggtctatg cgctcgacgc
     1381 ccgggcccgc tcggtcgtgg tgctggcgca gggccgggag acgaagcgga tcgacctcac
     1441 gccgctcggg gcggccgacc ccacagcact cgccgtcgac gggctggggg acctgttcat
     1501 cctcgacggc ggcaccggct gggtgttcgt cggcgatccg caggggaagc ggatcgcggt
     1561 cgtgcgcccg tcgaagatcg tgaccgatcg caccggggcg atcacggcga tcgaggtgga
     1621 cgctcaggga cgcctctacc tcgccggccg gcgctccggg gcggtggtgc gcctccgatg
     1681 attcgccgcc tggcgttcgc ctgcgggctc gccctgctcc tcctggcggc gccgctcccc
     1741 cccgccggga cggcgcgggc gcaggaagag gaggacctga cgaaggagct ggtggcgatg
     1801 cgcgccgcct tcgaccgggc gcgcgagcgc ctcgacgagc tcgacttcac ggcggccgtg
     1861 cgcgagctcg gcacgatcat cgagccgcgc gcccggacgc gcccttccga tctcgggttc
     1921 gaggagacgc ggcttctcgc ctccgcctac gatctgcggg cgcgggcgca gttcaacctc
     1981 ggcaacgccg cggcggccga ggccgacttc gggtccctgc tgcgcctcga tccgtcctat
     2041 tccatcgacc ggcagacgct gtccccgaaa gtcgtcgatc tcttcgaccg ggtgcgcgcg
     2101 aagacggtcg gcatcctgaa cctcacggtc gatccggcgc gggcgcgtgt gaaggtggac
     2161 ggggatccgg tcgacgccgg cccggagggg attggagtcc tgtccgggac ccacgagctg
     2221 cgcgtcgaga tggagggcta cgacccgcat gtcgagacga tcacggccgc cggcggcgcg
     2281 cgcctcgagc gccgggtgcg tctccgcccc aacaggcgtt cgttggagtt catcaccgtg
     2341 ccgacggggg tgaccgtgat gatcgacgac gcggcggccg gcacctcggc ggggccgccg
     2401 acgccggagg tggaggggct cgcggcgacc tacgggttcg acgcgcggaa ggcgtcggcg
     2461 ccgctgctcg tcccgaccgt cacgccgggg gagcatcgcg tctcgttcca gcgcgactgc
     2521 tacgaaacgc agtcggtcgt ggtccgggtc gagctcgatc cggagcagaa ccgccccatg
     2581 cgcttcgccc cggtggtcct gaaggaatcg cgcacccgcc tgcagatcac ctcggtgccg
     2641 gccggtgcgg aggtgcaggt ggacggcgtc ccgcagggga cgacgccgct gaccctcgaa
     2701 tcgctctgcg gcggcgatcg cgagatcacc gtggtccggc gcgaggtggg gcgctggagc
     2761 gagcgcgtgc ggctggcgac cggtcaggtg aacacgctcg acgtgcggct gcgcccgacc
     2821 cttctgtacc tcggcacctt ccgcctcgac gagtgggggc gggcggtctg gagcgacgag
     2881 gacaagccgc tcctcgacgc gctcggccgc ggtctcaaga ccctcaacgt ggcacgcgtg
     2941 ccggaggtgc agcagtcgat ccgcgacgcg gtgatccgct ggatgatcgc cgatccgcgc
     3001 gaggcgcgcg ccggcaccct cgtccccccc gacctgctgc gcgacgcggc ggagcgagcc
     3061 ggggccgatc tcatcctcgc cgggctgacg ctggccgacg atccggagcg ctcctggacc
     3121 ctgggtctct actcggcgct gcacacgacc ccggacgtcg tccggctgcg gctggaccgg
     3181 ccggccgggg tcgccgattt cgtcgcgcgg ctcgacagcg caccgcagca ggggtccacc
     3241 ctgtggggga tcggcctggc cgacagcgcg ctgccgccgg gaggaccggt cgtggcgcgc
     3301 gtgctggcgg gaagcccggc cgccaaggcc ggcgtgcggc tcggcgaccg ggtgacctcg
     3361 atcgggacga agaagacgac ctcgacccgg gaggcgctcg cggcgctcgc atcggaaagc
     3421 tcgcgcggcg gcggcgcgcg ctcggcggtc gtcgtgaccc tgcaagggac ggatgggacc
     3481 cgcaccaccc gcatcgcccc ggtcgaggcg ccggcccttc tgtcgctcac cgatccgcag
     3541 atcctctaca accgcgcgct cgtcgagtac cgcctgcgcg cccgcgccgc ggccgacgaa
     3601 accgcccgcg gcgtcgcggc gctcaacacc ggcctggcgc tgatgcactt ccgcgcctac
     3661 gacaaggcgc tcgccgaagg gctgcagcgg gccagcctgc cccccgggag cggcatcgcg
     3721 caggggacgg tggactacta tcgcggtctg tgcgcgcagc ggcgcggcga caccgacggc
     3781 gcgcgtgccg ccttccaggc ggcggtgaaa gcggccggct cgactctcga atcccccgag
     3841 ggcccatccg cttccgcggc ggcgacccgg gcgctgcagt cgctgcagta gcgagctcag
     3901 gaggctcccg ttgcttgacc cccgtcccac cttcctttac agtccgacga gcatgagcgg
     3961 gcggatgcgg gcgacagctt tcgggatcct cctggcggcg ctctctgccc cggccggctt
     4021 cgcgccgctg ttcggggcgg agaacgaggg ggccctcatc cacggcatca tctaccgctc
     4081 cgacgaggcg acccgcctcc agggggcgct cgtgaccgcc atcaacgtcc gcaccggccg
     4141 gcgctactcg agcatccaca ccggcgcgaa cggcgcctac gagatcgcgg gccttccggc
     4201 cgggacctac gacatcgcca tcgacacccc ggacgacaac ctgtacgtca ccgacggact
     4261 catcgatctg cacgagaacc agcggctgat gctctccctg tcgctcaaga agagcggcgg
     4321 ggcggtgccg ggcggtcccg cgcgcggcga gccgacgcag agcttcaccg acccgggggc
     4381 ggtgccgccg acctttgccc cgggtgcggc gacgccactg ccgcccgcgg ccgaagcctc
     4441 cggcgggaag tccgataaga aacccgaaaa gaagccgaag aagccggtcg gcacgaaagc
     4501 caagaccggt tcgggcgcga cggcgacgct ccgcctggag ccggcctcct ccttccccgt
     4561 ttcctgaagc ccgagcgacc attgaccgcc gagacggagc gcgtggcggc gctcgatccc
     4621 gcggcagagt acgccgccgt ccgcgaggag atcgacgcgg ccgtctcgcg ggtgctcgcc
     4681 tccgggcgct acgtcggcgg gcccgaggtg gaggggctcg aggcggagtt cgccgccttc
     4741 tgcggggtga cgcatgctgt ggcggtctcc accgggaccg acgccctgcg cttcgccctg
     4801 atcgcctccg ggctgcctgc cggtggagag gtggtgacga gccccttcac cttcatcggc
     4861 acgaccgagg cgatccacca ggcggggggg cgcgccatct tcgccgacat cctcccggac
     4921 acgttcgcca tcgacccggc gcgggtgaaa gcggccctca ccctccgcac cgccgccgtc
     4981 ctgccggtcc acctgtacgg acacccggcc gacatggatt cacttcaacc agaagcgggc
     5041 catcgacgac tcacaatcgt cgaagacgcc tgccaggcgc acggggcctc gctgcacggg
     5101 cgcatggtcg gggggatggg gacggccggg tgcttctcgt tctacccgac gaagaacctc
     5161 ggagcctgtg gcgagggggg gatggtgacg accggcgatg ccgcgatcgc ggcgcgggtg
     5221 cgtcgcctgc gcgatcacgg ccagtcggag aaatacctgc acgccgaaga ggggtacaac
     5281 ggccgcctgg acgccgtgca ggcggcgatc ctgcgggtca agctgcgccg cctctgggag
     5341 tggaacgagc ggcgccggtc gctcgcgacg ctgtacgccg accggctcgc ccccctggaa
     5401 gggcgcgggt ggctgcgccg gccggtcgag cggcccgggg tgaagcacgc gtggcatctc
     5461 tacgccgtgc gcattccctc cgaggcggcg gacctccgcg cggccgaccg gacgtcgcgg
     5521 cgggatcagg tccgtgcgcg acttctgggg caggggatcg aaaccggcat ccattacccg
     5581 gtgccgctgc accgccagcc cgcctatgcg tcgcttggac tcgccgaagg ctccttcccg
     5641 gaggccgagc gggcggcgcg cgaggtgctc accctcccga tccatccgca cctcagcgag
     5701 cggcaggcgg agcgggtggc cacagcgctc gccgcggcgc tcgagtcggc atgagccggc
     5761 ggcggcgcgc gcgggtgctg ctcgtcgccg gtgcgcggcc gaacttcatg aaggtcgcgc
     5821 cgctgctccg cgccctgcgc gggcggcccg accgcttcga gccgatcctg gtccacaccg
     5881 gccagcacta cgacgagacg atgtcggacg tcttcttcaa ggagctcggg atcccgaggc
     5941 cggaccggca cctcggcgtc ggatcgggga gccacgcggc gcagacggcc cgcatcatgg
     6001 aggccttcga gccggtcctg cacgatgcga agccggaccg cgtcgtcgtg gtgggggacg
     6061 tcaactccac cgccgcctgc gcgctggtgg cgtcgaagat ggcgcccccg gtgccggttg
     6121 cgcacgtcga ggcggggctt cgctccttcg accgcgccat gccggaggag atcaaccggc
     6181 tggtgaccga cgccctgtcc gatctgctct tcacgacgtc gccggatgcg gaccgcaacc
     6241 tgcgctcgga gggggtgccg cgccgccgca tccaccgcgt cggcaacctc atgatcgata
     6301 cgctccgggc gttcgtccgc cgcgccgacg gctcggacgt gctgcggcgg ctgcggctcg
     6361 cgccgccgta cgcgctcctg acgctgcacc gcccgtcgaa cgtggacgac gagacgacct
     6421 tccgccgcat cctcgccgcc ctcgaggaga tcggccgcgg cctgccggtc ctgttccccg
     6481 cgcacccgcg gacgacggag aggctccgcc ggctcggggc gggaaacggc acacgcggtc
     6541 tgcgcctcgt ggagccgctc ggctatctcg acttcctcca tctccagaag cgcgcctccc
     6601 tcgtcctgac cgattcgggc ggggtgcagg aggagacgac catcctcggc gtgccgtgcc
     6661 tgacgctgcg ggagaacacc gagcggccga tcacgctgac gcgcggcacc aaccgcctgg
     6721 tcggcaccga cacccgccgc atcgtgaccg aggcgcgccg ggccctgcgg aagagacgcg
     6781 gccgcgcccc gaccatcccc ctgtgggacg gccgtgccgc ggagcgcatc gccagggttc
     6841 tgggggcgga acgccgcgtc gaggattgac acgtgcaacc cgccccggac agaattgcgc
     6901 cactcgctct ggcttcgccg cgccggacgc tcccctggag tagtgtgaga gcggacgtcg
     6961 actcgacaga accgggatca cgacgccgcc gccctgcgaa gggcggcgcc ggtgtgatcc
     7021 gtcaacgctc catcgccgtc gccatcaagc gcgcgctccg ccgcgagcgg caccgcatcg
     7081 cggccgatct gcacgacgag ctcggccagg cgatggcggc tgcccactac aacctgtcgc
     7141 tcctcgaggc ctgggccatc ggcggcccgc gcgacgtgcg ccgcatcctg cgggaagtca
     7201 cccgcatcgt ctcccgcacc ctcggagaaa cccgccgcat cgccgccgac ctgcagccgc
     7261 gcgccatcga cggcaagggg ttcgagccgg cgctgcgcgg tctgctgaag tccttctccc
     7321 gccgcaccgg ggcccgcgcc cggctgcaga tcgaaggggg agcactgccg ctttgcctgg
     7381 gcgcgcagtc gctcctgttc cgcatcctgc aggagtcgct caacaacgtc gcggcgcacg
     7441 cgcgcgcccg gaacgtggcg atccgcctcg tctgctcgga cggcggcacc gtaatgagcg
     7501 tccacgacga cgggatcggg ctccgcgccg tcggccgccg cgctccgacc tccgggctgg
     7561 ggctcgccgg catgcgtcgg cgcgtgcgcg acgtgggcgg ctcgctgcgg ctccactcga
     7621 ccccggggcg cgggacgcgc ctcgtcgtca ccctgcaccg gcgtgagccg gtcccgggac
     7681 ctcgagagat ggcccgccga tgagcccgaa ccggaaaatc cgcgtgctcc tggccgacga
     7741 tcacaccctc gtccggcagg ggctgcgtgc gctcctcgag cgcgaagccg acatcaagat
     7801 catcggcgag gcgcgcaacg gccgcgaggc ggtcaaccgg gtcctggccc tcaagccgga
     7861 catcgtggtg atggacatct ccatgccgca cctgaacggg ttcgaggcga cccggcggat
     7921 cgcggccgag tgcccggaga cccgggtcgt ggccctctcg ttgcacaagg aggagcacca
     7981 cgtgcaggcg ctcctccggg cgggggcccg cggctacgtc ccgaaggacg cgccagcctc
     8041 cgagctcctg gccgcgatcc gggcggtgga cgggggaggg tgctacctcc acccccagat
     8101 ctccgccaag gtcgtggacg gctacctcga cgggaaccgg cccccggggg agagcgagcc
     8161 gctcactccc cgcgagcgcg aggtcctgca gctggtggcc gaggggcgca ccaaccgcct
     8221 gatcgcctcg gagctgcgcc tctcgatcaa gacggttgag gcgcaccggg cccggatcat
     8281 gaccaagctc aagatccaca acgcggccgg gctgacccgc tacgccatcg gccgggggat
     8341 catcggctcc gactgagctc cgacggctct gactgaacct acgacgcccg gttcacgggg
     8401 cggaccgtca ccttgaccct ctgaaaagcg gtctgctacg atccgggacg cccttcagag
     8461 gttcccatgt tccaagccca tccaccttcc ttcttcgacc tctaggtacc ttcacacgcc
     8521 ggcggtactg cgcagtaaac cgttgtgaag aaagaggttg ttaaaatgaa gaatgggacc
     8581 aggcgggacc tcaggcaccc gaagggaagt ctcgcccccc ctgcccaggc cgccggagcc
     8641 aggacgcggc cgggcggcag cggcgcggtc gtcctgccgg ggagggagga cgaggagtcc
     8701 ctcaaggtcg tcgagccggt ggccgccatc cccgccagcc gccgggcgcg ggccaaggcc
     8761 cgggcccgcc ggggcgcccg ccgcttcccc gtggagaagg tgatcgccga gtcggggcgc
     8821 tggctcgaca tcatcgcccg gggggaccgg ctgaccgggg cgcagaagaa gcgcgtcatc
     8881 aaagagacgg tcaagtcctt ctccacctac ttcaacaccg gcttcctggc ctaccgtaaa
     8941 tcggtcgccg aggcggagca gtacgcctcg atcgagtgga ccggccaggg gacgatgatc
     9001 gaggacatca ccggccggca gtacatcgac tgtctcgggg ggttcggcat ctacagcgcc
     9061 ggcatccgcc acccgaagat cgtcgaggcg gtccggaacc agctcgaccg gatgccgctg
     9121 tcgagccagg agctgctcga cccgctgcgc ggagcgctgg cggagctgct cggggagatc
     9181 gcccccggga acctccagaa gtgtttcttc atcaacaacg gcaccgacgc ggtggagggg
     9241 gcgatgaagc tggcgcgcct ctacaccgag aagtccgggt tcatctcgac gatccgcggc
     9301 ttccacggca agtcgtacgg gtcgctgtcg ctcatgggga aggcggagtt ccgcatcgcg
     9361 ttcgagccgc tgctccagga cgtctacttc gtcgagttcg gcgatgccga cgaggtcgaa
     9421 tggcagctca agaaggcgcg tgacgtcggg gcggacatcg ccgcggtcgt cgccgagccg
     9481 gtgcaggggg aggcgggggc gatcgtcccg ccggacgact actggccgcg cctcagggcg
     9541 atctgcgacg agttcgacgt gctgctcatc gcggacgagg tccagaccgg catggggcgg
     9601 accgggaaga tgttcggggt cgatcactgg gacgtggtgc ccgacatcat gtgcctcggg
     9661 aaatcgctcg gcggcggcgt catgccgctg tcggccttcg tgtcgacgcc ggagatctgg
     9721 aaggtgctcg agcagaatcc gttcctgcac tcttcgacgt tcggcggcaa cccgctcgcc
     9781 tgcgccgccg gcatcgcggc gatccacgtg acgctgagcg aagacctgcc ggggcaggcg
     9841 gcggagcggg gggcggagtt catccagggg ttgcgcgaca tccaggcgcg ctactccgac
     9901 gtcatcgtcg acgtgcgcgg caaggggttg ctcatcggcg tcgagttcaa cgacaccgag
     9961 ttcggctact cggtcgccgc cgggctcttc cgccgcggcg tgctggtcgc cgggacgctc
    10021 ctcaacgcca aaacgatccg catcgaaccg gc
//
LOCUS       DASRIQ010000270         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1166222, whole genome shotgun sequence.
ACCESSION   DASRIQ010000270 DASRIQ010000000
VERSION     DASRIQ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166222"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFD06_06655"
     CDS             <1..417
                     /locus_tag="VFD06_06655"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpC"
                     /protein_id="HZN03253.1"
                     /translation="ASDRDSAYDDRREVVLRELRAALHPEVINRVDEIIVFDALTDEN
                     LLAISRLMIRQLNTSLSERGIRIRVTDDAHRWLIEKTCGDRSYGARPLRRAIQKHIED
                     VLSESLILGRLPERGEIEIFVDQERLAFREPIGITS"
     gene            674..>2441
                     /locus_tag="VFD06_06660"
     CDS             674..>2441
                     /locus_tag="VFD06_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZN03254.1"
                     /translation="MVDGNVRVSDAAFFSNIRLRTGTPYDEEEVRREFRRMWDLDLFD
                     DIAVESRRRPDGTVDLIFHVRDRPLLGSVVFTGMKAITETSILERLQQGKAEVRRGQP
                     VDFTTLRHAESLIGHMLAEKGYLDARVKARLGPVAQGQRDVTFEIHEGAKTKIKEIDF
                     VGNTVYSDGELRKTLKLTKQAFWLTSWASSKPLYHPAKFDQDAEGIRTAYRSRGYLDV
                     VIQPEVVEIESEVDRKERQEAGAPAPDAKAPPQRVAFDPMVFQDLEEEPIEAPPPPPP
                     GETEKEKKKRLKKEAKAREQQTSGPKKRWVYLTVPIVEGRPYRLGSVEIEGNTVFSDR
                     QVMARFPLRGGMIYNDALVKAATKRMEDDYGEVGYFYVSIDPQVRKHDGIADLSLEIT
                     EDRKYFVDRIDFSGNDSTRDSVLRREMRLQEQQLFNVKLMRLGVRKIAQLGYWQVEGD
                     PVVTPEQESGQVQIKVQGTEASRNEIQVGGGVSGLDGGFFQGSYSTRNFLGRGEVFSA
                     FLQTGQRGSRYALNFTEPWFLGRPWSLGFSLFRRQAVYTGYRREGQGGAVNFGRLLGA
                     FSRFDVAYTLEDITFVPTSAAFG"
BASE COUNT          417 a          847 c          842 g          335 t
ORIGIN      
        1 gcatccgacc gggactccgc gtacgacgac cgacgcgagg tggtgctgcg cgagctgagg
       61 gcggcgctcc atccggaggt catcaatcgc gtcgacgaga tcatcgtctt cgacgcgctc
      121 accgacgaga acctgctggc gatctcccgt ctgatgatcc gccagctcaa caccagcctc
      181 tccgagaggg ggatccggat ccgcgtcacc gacgacgctc accgctggct gatcgagaag
      241 acctgcggcg atcgctcgta cggcgcgcgc ccgctccgcc gcgccatcca gaagcacatc
      301 gaggacgtcc tctcggaaag cctcattctg gggcggctgc ccgaacgcgg ggagatcgag
      361 atcttcgtcg accaggaacg gctcgccttc cgcgagccga tcggcatcac ctcctgaacc
      421 gaaaccgagg cgctccgttc gcccccttgt cctgctgatc gccgcgatcc tgctgccgtg
      481 gccgggcccg acggccgcgc aggacgtccc tccgccgccg gccggcgagg agggagagtc
      541 cgcgcccctt ccgccacccc ccacgcacga cgagcgggcc caggagccgc ccgccgcgcc
      601 cgcccccgac cgggaggcgc ccgcggagga ggagccgccg cagggggccg ggccggccac
      661 catcggccgc atcgtggtcg acggcaacgt gcgcgtctcc gacgccgcct ttttctccaa
      721 catccggctg cgcaccggca ccccctacga cgaggaggaa gtgcggcggg agttccggcg
      781 catgtgggat ctcgatctgt tcgacgacat cgccgtcgag tcgcgccggc gccccgacgg
      841 cacggtcgac ctgatcttcc acgtccgcga ccggccgctg ctcggcagcg tcgtcttcac
      901 cggcatgaag gcgatcaccg agacctccat cctcgagcgg ctgcagcagg ggaaggcgga
      961 ggtgcggcgc ggccagccgg tcgacttcac gacgctgcgg cacgcggagt cgctcatcgg
     1021 ccacatgctc gcggagaagg ggtacctcga cgcgcgcgtg aaggcccgcc tcgggcccgt
     1081 ggcgcagggg cagcgggacg tgaccttcga gatccacgag ggggccaaga ccaagatcaa
     1141 ggagatcgac ttcgtcggca acaccgtcta ttccgatggg gagctgcgca agacgctgaa
     1201 gctcacgaag caggcgttct ggctgacatc gtgggcctcg agcaagccgc tgtaccaccc
     1261 cgccaagttc gaccaggacg ccgaggggat ccggaccgcg taccgatcgc gcggctacct
     1321 cgacgtggtg atccagccgg aggtggtcga gatcgaatcg gaggtcgatc gcaaggagcg
     1381 gcaggaggcc ggcgccccgg ccccggacgc caaggccccc ccgcagcggg tcgccttcga
     1441 tccgatggtg ttccaggacc tcgaggagga gccgatcgag gcgcccccgc cgccgccccc
     1501 cggcgagacg gagaaggaga agaagaagcg cctgaagaag gaggccaagg cccgtgagca
     1561 gcagaccagc ggcccgaaga agcgctgggt ctacctcacg gtgccgatcg tcgagggacg
     1621 gccgtaccgg ctcggcagcg tcgagatcga ggggaacacc gtcttcagcg atcgccaggt
     1681 gatggcccgc ttcccgctgc gcggcgggat gatctacaac gacgccctgg tgaaggccgc
     1741 gaccaagcga atggaggacg actacggcga ggtcggctac ttctacgtgt cgatcgatcc
     1801 tcaggtgaga aagcacgacg gcatcgccga cctgagcctc gagatcaccg aggaccgcaa
     1861 gtacttcgtg gaccggatcg acttctccgg caacgacagc acgcgcgaca gcgtcctgcg
     1921 gcgggagatg cggctgcagg agcagcagct gttcaacgtc aagctgatgc ggctgggggt
     1981 gcgcaagatc gcccagctcg gctactggca ggtggagggg gatccggtgg tgacccccga
     2041 gcaggagagc gggcaggtcc agatcaaggt gcaggggacc gaagcgagcc gcaacgagat
     2101 ccaggtgggg ggcggtgtct caggcctcga cggcgggttc ttccagggct cctactcgac
     2161 gcgcaacttc ctgggccgcg gggaggtctt ctcggcgttc ctgcagaccg gacagcgcgg
     2221 cagccgctac gccctcaact tcaccgagcc gtggttcctc ggccgcccgt ggtcgctcgg
     2281 cttctccctc ttccgccggc aggcggtgta caccggctac cgccgggaag ggcagggagg
     2341 ggcggtgaac ttcgggcgcc tgctcggcgc gttctcgcgc ttcgacgtcg cctacaccct
     2401 cgaggacatc accttcgtcc ccacctcggc ggcgttcggg a
//
LOCUS       DASRIQ010000271         5524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3229546, whole genome shotgun sequence.
ACCESSION   DASRIQ010000271 DASRIQ010000000
VERSION     DASRIQ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229546"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VFD06_06665"
     CDS             <1..1502
                     /locus_tag="VFD06_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03255.1"
                     /translation="DGYDIVLTEAHTLSSRGVRMKMKRSPAIARRPDEGRGTAWMFRP
                     QPPDVLRDAGAGPPESTVLLVPPTRPDGSGGDAPGRSPGAGFLGSADGEIAQLVGAGL
                     LPGLEGAGGDAVRDTTVTLRAPLSESIAWSVAGRSLRNEAPAEGDEVISGRSDRLALG
                     MSLDGGAEARVRGRARAGYADDAAGTDRISDRVIEAEGDVRLADTNTRLAVAVQVWST
                     HSEADAATAGEGAAIYAGSRSRVGDRLDVDYGFEYRADTLTRRPRAVPRIGFGYARPA
                     GDPLTLRADLLLDATSPGARVAITARPRGQVDLAAALTFLPANALDAVAPAAASDPAF
                     FRSRSAAVEPAGARSADRRALELSVARSFGALRGSLSGSVGRTGRRAVPLVEDAALPV
                     LSYGEERFYETRLGVGYLRSSTRMELGYRRVAAAEGDPEGTEGAGLAYSRMDVLVAQG
                     LPSPRLLSGARLSAMLSWQGLDYERLDAGAGVLLCGLATRLTGGVGLSF"
     gene            1516..4305
                     /locus_tag="VFD06_06670"
     CDS             1516..4305
                     /locus_tag="VFD06_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN03256.1"
                     /translation="MRLRRTVALAVTLLLLAWGAHSFTSRLARLEPEIGVEWIDTPRG
                     AVADWVVPREPGWRAGLRPGDRLLEIDEEPVRMVVEAERSVDAVERDGTLLFRVDRNG
                     ERVDCPVRPVWVGGVDAVDYYLLLVALTFLGVGLIVWLRATRARAAVPFALHCQAMFL
                     YLVHKPTGRGGMLDLATYWGDLAGLLLAPALFVHINLALTDDDRGAAGRRLRRAALYL
                     PPFLLFLINLTLIAFEGAYRFDDPVAAIRLKERLEQLYVAVYLIAGIVMAVRSFVAAE
                     KLQTRWQLKWMAWGSVCGFLPPALLYLAPLSLGVRIGHWSQLSVLPMALVPLSFAAAV
                     VRYRLLDLDLFLKRGTVAIGLLLSFASAYAACYVVMDRMAGGASRPGFNVALIGATLM
                     MVIFYPRLHRRLRAWVDQFFYRERHDFRRTLVEFGAALNRELSLPALVTRIEGRIERT
                     FNAAAVRVLVRDRDGALFSDGAGTLAAEDPLVGLLAGVDWLSLRDHPGALDLPSGARL
                     LEESGVEYLFPLLVEGEILAIVGVAPPRDGVPFTSEDLQLLVSFGGHAAVAIAGARLY
                     SALAEKVSEIESLREFNESILESSRVAILVLDPEGRVAGVNRAFEALSGTPRAHLAGR
                     PVAEALPARAARGLPACGEEGARLDRAPWRSADGTSAIVNLTRSPLRDGRGRLLGTVV
                     TLDDVTDQVRREEDLQRREHLASIGLLASGVAHEVNTPLTGISSYAQMLLQGRNQDDP
                     AYPLLRKIQQQTERASGIASSLLNFARQADGDYQPVDVGDMAQETLQLFEPHLKGRRI
                     ELHRHIEPSLPAVTGNRGRLQQVLMNLLLNAADATPDGGAIAVRATSSSGRIRLTVSD
                     TGVGIPAEHLDRIYDPFFTTKPRGRGTGLGLSVSYGIVKEHAGTLIAESAPGEGSRFV
                     ISLPVIEAGVRRVTA"
     gene            4302..>5524
                     /locus_tag="VFD06_06675"
     CDS             4302..>5524
                     /locus_tag="VFD06_06675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN03257.1"
                     /translation="MSEGGSVDVGSAVEAERVVESAPREAGRILLIDDEPIIQEVLGA
                     ILAPEGHRLTVVADAERGLETLEGEDYDLVILDLMLPGMGGLEALTRIRQRDPDQVVL
                     MLTAYGSVETAVQAMRMGANDYLTKPFQNEEVLRSVRHGLRHRRLALENRTLRRALQG
                     KTGSWGLVGKSRSMQALYSLIEQVAPSRSTVLIQGESGTGKELVAHAIHLRSGRPAES
                     FVVVNSGSMPADLLESNLFGHVKGAFTGAVAAKKGLFEVANGGSIFFDEISSIRPEVQ
                     AKLLRVIQEKEFLPLGAVQSEHVDVRIIAATNIDLMELVRRGEFREDLYYRLNVITLL
                     LPSLRDRREDIPPLVEHFIEKYAEENRKDIHSLAPEAMGRLIDHPWRGNVRELENVIE
                     RAVVLAQGPEIGVDLL"
BASE COUNT          728 a         1967 c         2021 g          808 t
ORIGIN      
        1 aggacggcta cgacatcgtc ctgaccgagg cgcacacgct gtcgagccgc ggggtgcgca
       61 tgaagatgaa gcgcagcccg gcgatcgcgc gccggccgga cgaagggcgc ggcaccgcgt
      121 ggatgttccg tccgcagccg ccggacgtgc tgcgcgacgc gggggccggt ccgccggaat
      181 cgaccgtcct gctcgtcccg ccgacgcggc cggacggcag cgggggggac gcgccgggac
      241 ggagccccgg ggccggcttt ctcgggagcg cagacggcga gatcgcgcag ctcgtcggcg
      301 cgggcctcct gcccggtctt gagggggcgg ggggggatgc ggtccgcgac acgaccgtga
      361 cgctgcgagc ccccctgtcg gagtcgatcg cctggagcgt cgccggccgc agcctgcgaa
      421 acgaggcccc cgcggagggg gacgaggtga tctccggccg ctccgatcgt ctggcgctcg
      481 gcatgagcct ggatggaggg gcggaggctc gggtgcgcgg cagggcgcgc gccggctacg
      541 cggacgacgc cgcggggacg gaccggatct ccgatcgagt gatcgaggcg gagggggacg
      601 tgcggctggc cgacacgaac acccgtctgg cggtggccgt gcaggtttgg agcacgcaca
      661 gcgaggccga cgccgccacg gccggcgagg gggcggcgat ctacgccggc agccgctcgc
      721 gggtcggcga tcgcctcgac gtcgactacg gcttcgagta ccgcgccgac accctgacac
      781 ggcggcctcg ggcggtgccg cggatcggtt tcggatacgc aaggccggcc ggcgacccgc
      841 tgacgctgcg ggccgacctg ctcctcgacg cgacatcccc cggggcgcgg gtggcgatca
      901 cggcgcgccc gcgcgggcag gtggacctcg ccgccgcgct gacgtttctg cccgcgaacg
      961 cgctcgacgc cgtcgccccg gccgccgcct cggacccggc gttcttccgg tcccggtcgg
     1021 cggcggtcga gccggcgggg gcccggagcg ccgatcgccg tgcactcgaa ctgtcggtgg
     1081 cgcgaagctt cggggcgctt cgcggcagcc tcagcggctc ggtcggacgc accggccggc
     1141 gcgccgtgcc gctggtggag gacgccgcgc tgccggtcct gtcgtacggc gaggagcgct
     1201 tctatgagac gaggctcggg gtcggatacc tccgctcctc cacgcggatg gagctcggct
     1261 accgccgcgt cgccgcggcg gagggtgatc cggaagggac cgagggcgcg ggtctcgcgt
     1321 acagccggat ggacgtcctg gtcgcgcagg gtctgccgtc tccgcgcctg ctctccggcg
     1381 cgcgcctgag cgccatgctc tcgtggcagg gactcgacta cgaacgcctc gacgccggag
     1441 ccggcgtcct gctctgcggt ctggccacgc gcctgaccgg tggagtcggc ttgtccttct
     1501 gacgccggat cccccatgcg cctccgtcgc accgtcgccc tcgccgtcac cttgctcctg
     1561 ctcgcctggg gggcccattc gttcacctcc cgcctcgcgc gcctggagcc ggagatcggc
     1621 gtcgagtgga tcgacacccc gcgcggcgcc gtcgccgact gggtcgtgcc gcgcgagccg
     1681 ggctggcggg ccgggctgcg gccgggcgac cggcttctcg agatcgacga ggagccggta
     1741 cgcatggtgg tcgaggcgga gcgctcggtc gacgccgtgg agcgcgacgg gacgctgctg
     1801 ttccgcgtcg atcgcaacgg cgagagggtg gactgcccgg tgcgcccggt ctgggtcggc
     1861 ggggtcgacg cggtcgacta ctacctcctc ctcgtcgcgc tgacctttct cggcgtcggc
     1921 ctgatcgtct ggctgcgcgc gacgcgggcg cgggcggcgg tgccgttcgc cctccactgc
     1981 caggcgatgt tcctgtacct cgtgcacaag ccgaccggac gcggcggcat gctcgacctc
     2041 gccacgtatt ggggggacct cgcgggactg ctcctggcgc cggcgctctt cgtccacatc
     2101 aacctggcgc tgaccgacga cgatcgcgga gcggccggcc ggaggctgcg gcgcgccgcg
     2161 ctctacctgc cccccttcct cctgttcctc atcaatctga cgctgatcgc cttcgagggg
     2221 gcctaccgct tcgacgatcc ggtggcggcg atccgcctca aggaacgtct ggagcagctg
     2281 tacgtcgccg tgtacctcat cgccggcatc gtcatggcgg tgcgctcctt cgtcgccgcc
     2341 gagaagctgc aaacgcgctg gcaattgaag tggatggcct ggggcagcgt ctgcggcttc
     2401 ctgcctccgg cgctcctgta cctcgcgccg ctgtcgctcg gcgtccggat cggccactgg
     2461 agccagctgt cggtcctgcc gatggcgctg gtaccgctgt cgttcgccgc cgcggtcgtg
     2521 cgttatcggc tgctcgacct cgacctcttc ctcaagcgcg gcaccgtcgc catcggcctg
     2581 ctgctgtcgt tcgcgtcggc ctacgccgcc tgctacgtcg tcatggatcg gatggccggc
     2641 ggcgcctccc gccccggctt caacgtcgcg ctcatcggcg ccaccctgat gatggtgatc
     2701 ttctatccgc gcctgcaccg gcgcctgcgc gcctgggtcg accagttctt ctaccgcgag
     2761 cgccacgact tccggcgcac gctcgtcgag ttcggcgcgg cactcaaccg cgagctcagc
     2821 ctgccggccc tcgtgacccg tatcgagggg cggatcgagc ggaccttcaa cgcggctgcg
     2881 gtgcgcgtcc tcgtgcggga ccgggatggc gccctcttca gcgacggggc cgggaccctc
     2941 gccgcggagg accccctcgt cgggctgctc gcgggggtgg actggctgtc gctgcgcgac
     3001 catccgggag ccctcgacct gccgagcggc gcgcgcctgc tcgaggagtc gggggtggag
     3061 tacctcttcc cgctcctggt cgagggggag atcctggcca tcgtcggggt cgcgccgccg
     3121 cgggacggcg tcccgttcac gagcgaagat ctccaactgc tggtgtcctt cggagggcac
     3181 gcggccgtgg cgatcgccgg ggcgcgcctc tacagcgccc tcgccgagaa ggtctcggag
     3241 atcgagtcgc tccgggagtt caacgagagc atcctcgagt cgagccgcgt ggcgatcctc
     3301 gtcctcgacc cggaggggcg tgtcgccggg gtcaaccggg cgttcgaggc gctctcgggg
     3361 acgccgcgcg cgcacctcgc gggacgcccg gtcgccgagg cgctgccggc gcgcgcggcc
     3421 cgcggcctcc cggcgtgcgg cgaggagggg gcgcggctgg accgcgctcc gtggcgctcc
     3481 gccgacggca ccagcgccat cgtcaacctg acccggtcgc cgctgcgcga cggccgcgga
     3541 cggctgctcg gcaccgtcgt gacgctcgac gacgtcaccg atcaggtgcg gcgcgaggag
     3601 gatctccagc ggcgcgagca cctcgcctcc atcggcctgc tcgcctccgg cgtggcacac
     3661 gaggtcaaca cgccgctgac cgggatctcc tcctacgccc agatgctgct gcagggccgg
     3721 aaccaggacg acccggcgta cccgctcctg cgcaagatcc agcagcagac cgagcgcgcc
     3781 tccgggatcg cctcgagcct cctcaacttc gcgcggcagg cggacggcga ctaccagccg
     3841 gtggacgtcg gcgacatggc gcaggagacg ctgcagctgt tcgagccgca cctcaaggga
     3901 cggcggatcg agctgcaccg ccacatcgag ccgtcgctcc cggcggtcac cggcaatcgc
     3961 ggccggctcc aacaggtctt gatgaacctg ctgctcaacg ccgccgacgc gaccccggac
     4021 ggcggcgcga tcgccgtccg cgcgacgagc tcgtcgggac ggatccggct caccgtcagc
     4081 gacaccgggg tcggcatccc ggccgagcac ctcgaccgca tctacgatcc gttcttcacc
     4141 accaagcccc gggggcgcgg cacggggctc ggactgtccg tctcgtacgg catcgtcaag
     4201 gagcacgccg ggaccctgat cgcggagagc gctccgggtg aggggtcccg tttcgtcatc
     4261 agcctgccgg tcatcgaagc cggcgtcagg agggtcaccg catgagcgaa gggggcagcg
     4321 tcgacgtcgg atccgccgtg gaggcggagc gggtggtgga atccgctccg agggaggccg
     4381 gccgcatcct actcatcgac gacgagccga tcatccagga ggtgctcggc gccatcctgg
     4441 cgcccgaagg gcaccggctg accgtcgtcg ccgatgccga gcgggggctc gagaccctcg
     4501 agggcgagga ctacgacctg gtcatcctgg atctcatgct ccccgggatg ggagggctcg
     4561 aggccctcac ccggatccgg cagcgcgacc ccgaccaggt ggtgctcatg ctgaccgcct
     4621 atgggtcggt cgagacggcc gtccaggcga tgcgcatggg ggcgaacgac tacctgacga
     4681 agccgttcca gaacgaggag gtgctccggt cggtgcggca cggcctgcgg caccggcgcc
     4741 tggcgctcga gaaccggacg ctgcggcggg cgctccaggg gaagaccggc tcctgggggc
     4801 tggtcggcaa gagtcggtcg atgcaggccc tgtactccct catcgagcag gtggcgccca
     4861 gccgctcgac cgtcctcatc cagggggaga gcgggaccgg caaggagctg gtggcgcacg
     4921 ccatccacct gcgctccggc cggccggcgg agtcgttcgt cgtggtgaat tccgggagca
     4981 tgccggccga cctcctcgag agcaacctct tcggccacgt gaagggggcg ttcaccgggg
     5041 cggtggcggc gaagaagggg ctgttcgagg tggccaacgg tggctccatc ttcttcgacg
     5101 agatcagcag catccggccc gaggtgcagg cgaagctcct gcgggtcatc caggaaaagg
     5161 agttcctgcc gctcggggcg gtgcagagcg agcacgtgga cgtgcgcatc atcgccgcca
     5221 ccaacatcga cctcatggag ctggtgcgcc ggggggagtt ccgcgaggac ctctactatc
     5281 ggctcaacgt gatcaccctg ctactgccct cgctccggga ccggcgcgag gacatcccgc
     5341 cgctggtgga gcacttcatc gagaaatacg ccgaggagaa tcgcaaggat atccacagcc
     5401 ttgcacccga agccatgggc aggctcatcg atcacccctg gagggggaac gtccgcgagc
     5461 tcgaaaacgt gatcgagcgg gccgtggtcc tcgcccaggg gccggagatc ggggtcgatc
     5521 tcct
//
LOCUS       DASRIQ010000272         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1677885, whole genome shotgun sequence.
ACCESSION   DASRIQ010000272 DASRIQ010000000
VERSION     DASRIQ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677885"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFD06_06680"
     CDS             <1..468
                     /locus_tag="VFD06_06680"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03258.1"
                     /translation="SIYPLLMRLRSLGLVQERPHAEGRRKSVFLSLTAEGRAALAQWI
                     LPPLPDVVVGVPSDPLRTRLGFLVVLSPAQRRRFLADAAAGLERQRSEIEADERRHRK
                     RGDRYGILLARGATAMQAARIEWLQEVSRELEPVTRDRRTTASRGGSRRRARS"
     gene            525..854
                     /locus_tag="VFD06_06685"
     CDS             525..854
                     /locus_tag="VFD06_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN03259.1"
                     /translation="MPEKAAGLVQGTLDMLILKTLALEPMHSYDIGARLARVSQGVFQ
                     VNAGSLFPAFRRLERAGLIRGEWRATENNRRARYYIVTARGLRRLGAETREWRRLAGA
                     IARILEA"
     gene            856..>3355
                     /locus_tag="VFD06_06690"
     CDS             856..>3355
                     /locus_tag="VFD06_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN03260.1"
                     /translation="MDAAGRFLRGLLALFRRDRLERDLDEELRAYADAATDDKTRGGM
                     DRGAAQRAVRLEMGGLDAVKEQVRDAGWETGFESFVRDVRYGARILRRHPGFSIAAIL
                     TLALGIGANTALFSVVNAVLLRPLPYPDSDRLVTLEGSHSLPDIVDLAEMSSSMTVMG
                     TAARWDFDLVGEGEPRRVVGELAGEDLFPALGVQAALGRTFDASDDRARAPVVVIGDG
                     FWRSHLGADPGVVGRRLQLSGALYEVIGVMPPHFRSPFFGSEAQVWIPFRTGYPEGAS
                     ARGAHFTLPVARLASEATLPSAQAELTLIGRRIAEKNPTEARAFVARMLRDRVVGDVR
                     QPLLLLLASVALVLLVACANYASLLLARGAGRGGEMRLRLALGAARGRLVRQLVTESA
                     LLSLLGGLAGAALAGAGLRFLLALRPAGLPALNPITLDGRALGFALLASLVTGVLFGL
                     APAWQVARGGERLQAPGATMVATSALRRGLVVAQLSLALVLLTGAGLLLRSFWILRNQ
                     PSGFEPQGVLTMRLSLPAARYESIEAQQAFLARLEEGVRGLPGAGAAGLVTELPLSGW
                     RMMHNMIVEGDQPVPEGQEPEIYTHEVSPGYFAAAGTPILRGRGVRESDTPTAPLVGV
                     VNEAFVRRFSPDRDPLGLRARWARGAPDAWMTIVGVAADARFEDLGEAQEPTIYTPFL
                     QKQQPWKRWAAIVVRPAAGDPMTFAEPVRQAVRRLDAALPITDVKPMSAVMLDSMSDR
                     RFHLVLLSTFAGLAFVLAVVGVYGVLAHLVAQRSREVGVRMALGARRLDVLWLMLRQG
                     LPLIAGGVAFGAIGAMAATRLLRGLLYGVSAGDPA"
BASE COUNT          396 a         1209 c         1235 g          515 t
ORIGIN      
        1 tcgatctacc cgctgctgat gcggcttcgg agcctcgggc tcgtgcagga gcgccctcac
       61 gcggaagggc gccgaaaaag cgtctttctg agcctcaccg ccgaggggcg ggccgcgctc
      121 gcccaatgga tccttcctcc cctgccggac gtggtcgtcg gcgtcccctc ggatcccctg
      181 cggacccggc tggggtttct cgtcgtgctc tccccggcgc agcgacggcg ctttctcgcc
      241 gacgccgcgg cgggtctcga acggcagcgt tccgagatcg aggcggacga gcggcggcac
      301 cgcaagcggg gcgaccgcta cggcatcctg ctcgcccgcg gggcgacggc gatgcaggcg
      361 gcgcgcatcg aatggctcca ggaagtctcg cgggagctcg aaccggtgac ccgcgaccgt
      421 agaacgacgg cgtcccgcgg tggatcgagg cggcgcgccc gctcttgaca atcccggcgc
      481 gaggagtaga gtctcaccac tagaccgtct agtggaggag tccgatgccg gagaaagccg
      541 ccgggctcgt gcagggcacg ctcgacatgc tcattctgaa gaccctggcg ctcgagccga
      601 tgcacagcta cgacatcggc gcccgcctcg cccgcgtcag ccagggggtg ttccaggtca
      661 acgccggatc gctcttcccc gccttccggc ggctcgagcg ggccggcctc atccggggcg
      721 aatggcgcgc caccgagaac aaccgccgcg ccaggtacta catcgtcacc gcgcgcggcc
      781 ttcgacggct cggggccgag acgcgggagt ggcggcggct cgcgggggcc atcgcccgca
      841 tcctggaggc ttgagatgga cgccgcgggc cggttcctcc gcgggcttct cgcgctgttc
      901 cgtcgggacc gcctcgagcg ggatctcgac gaggagctgc gcgcctacgc cgacgccgcg
      961 acggacgaca agacccgcgg tggcatggat cgcggcgcgg cgcagcgggc cgtccgcctg
     1021 gagatgggcg gcctcgacgc cgtcaaggag caggtgcgcg acgccggctg ggagaccggc
     1081 ttcgagtcgt tcgtgcggga cgtgcgctac ggcgcgcgca tcctgcgccg gcatcccggt
     1141 ttctccatcg ccgcgatcct gacgctggcg ctcgggatcg gcgccaacac cgccctgttc
     1201 agcgtggtga acgcggtgct cttgagaccg ctgccgtatc ccgactccga ccgcctggtg
     1261 acgctcgagg ggagccactc gcttccggac atcgtcgatc tcgcggagat gtcctcctcg
     1321 atgacggtca tgggcaccgc cgcgcgatgg gacttcgacc tggtcggcga gggggagccg
     1381 cggcgcgtcg tgggcgagct ggccggcgag gatctgttcc ccgcgctcgg agtccaggcg
     1441 gccctcggac ggaccttcga cgcctcggat gaccgggcgc gcgcgcccgt cgtcgtgatc
     1501 ggcgacgggt tctggcgcag ccacctcggc gccgatccgg gcgtcgtggg ccgacgcctg
     1561 cagctcagcg gcgcgctcta cgaagtgatc ggcgtgatgc cgccccattt ccgatccccc
     1621 ttcttcggat ccgaggcgca ggtgtggatt cccttccgca ccggctatcc cgagggggcc
     1681 tcggcgcgcg gagcgcattt cacgcttccc gtggcgcgtc tcgcctcgga ggccacgctt
     1741 ccctccgcgc aggcggagct gactctgatc gggcgccgca tcgccgagaa gaaccccacg
     1801 gaggcgcgcg cattcgtcgc ccggatgctg cgcgatcgcg tcgtggggga cgtgcggcag
     1861 ccgctcctgc tgctgctcgc gtccgtggcg ctcgtcctgc tcgtggcctg cgccaactac
     1921 gcttcgctcc tcctggcgcg gggcgccgga cgcggggggg agatgcggct gcggctcgcc
     1981 ctcggcgccg cgcgcggccg gctggtccgc cagctcgtca ccgagagcgc cctgctgtcc
     2041 ctcctcggag gcctggccgg cgccgcgctg gccggcgccg gtctccggtt cctgctcgcg
     2101 ctcaggcccg cgggccttcc ggccctgaat cccatcaccc tggatgggcg tgcgctgggg
     2161 ttcgcgctcc tcgcctcgct cgtcaccggc gtcctgttcg gcctcgcacc cgcctggcag
     2221 gtggcgcgcg ggggcgagcg cctgcaggcg ccgggggcga ccatggtcgc cacctcggcg
     2281 ctgcggcgcg gcctcgtggt ggcgcagctc agcctcgcgc tggtgctgct gacgggagcc
     2341 gggctcctgc tgcgcagctt ctggatcctc cgcaaccagc cgagcggctt cgagccgcag
     2401 ggggttctga cgatgcggct gtcgctgccg gcggcgcgct acgagtcgat cgaggcgcag
     2461 caggcgttcc tggcgcgcct cgaggagggg gtgcgcgggc tcccgggcgc cggcgcggca
     2521 ggcctggtca cggagctgcc gctcagcggc tggcggatga tgcacaacat gatcgtcgag
     2581 ggggatcagc cggttccgga aggacaggag ccggagatct acacgcatga ggtgagtccc
     2641 ggctatttcg cggcggccgg aactccgatc ctccggggcc ggggggtccg cgaaagcgac
     2701 acgcccacgg caccgctcgt gggcgtggtc aacgaggcgt tcgtccgccg cttctcgccg
     2761 gaccgcgatc cgctgggatt gcgggcgcgc tgggcccgcg gcgccccgga cgcctggatg
     2821 acgatcgtcg gggtggccgc cgatgcgcgc ttcgaggacc tcggcgaagc gcaggagccg
     2881 acgatctaca cgccgttcct tcagaagcag cagccctgga agcgctgggc ggcgatcgtc
     2941 gtgcgcccgg cggcgggcga cccgatgaca ttcgcggagc cggtgcgcca ggcggtgcgg
     3001 cggctcgacg ccgcgctgcc gatcaccgac gtgaagccga tgtcggccgt catgctggac
     3061 tcgatgagcg atcggcgctt ccatctcgtc ctgctgtcca ctttcgcggg gctcgcgttc
     3121 gtcctcgccg tcgtcggcgt gtacggcgtg ctggcccacc tcgtcgccca gcgctcgcgc
     3181 gaggtggggg tgcgcatggc gctcggcgcc cggcggctcg acgtgctctg gctcatgctg
     3241 cgccaggggc tgccgctcat cgccggcgga gtcgccttcg gggccatcgg agccatggcc
     3301 gccacgcgac tcctgcgcgg ccttctctac ggcgtgagcg ccggcgatcc cgcga
//
LOCUS       DASRIQ010000273         6324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3229672, whole genome shotgun sequence.
ACCESSION   DASRIQ010000273 DASRIQ010000000
VERSION     DASRIQ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229672"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VFD06_06695"
     CDS             <1..1380
                     /locus_tag="VFD06_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZN03261.1"
                     /translation="DGALLSAAWIGVALSLVVLCGYANALILAALWALYFSFVSIGQD
                     WYGYGWEIQLLETGAVAILLCPLLDGRPFPRRPPPFAAILLLRWLAFRIYLGAGLIKI
                     RGDACWRDLTCLVHHYETQPIPNPVSRFYHFMPLWFHKGGVLYNHLIELAAPWFLFGP
                     RLLRLTAGSLMVAFQVILMTSGNLSFLNYLTIVPCLAAFDDAFLRRLLPRVVTRSADA
                     AAAAHAAAATTVAHDAAAAATAGPAPEAIGAPPQAALLKPSGAGRAHEAIAWAYAALV
                     AVLSLGPVGNMLSSRQIMNTSFDRLHLVNTYGAFGSVGEVRREIVFEGTADDAVTAAT
                     VWKEYDFWCKPGTPTRRPCLIAPFQPRLDWQIWFAAMSGPERYPWTLHLVAKLLRNDA
                     GALSLLSTNPFPDRPPRFIRAAYYRYQFAPPGEPDGAWWRRTYLGEWLPPLALEDPRL
                     IRFLQAYGW"
     gene            complement(1399..2199)
                     /locus_tag="VFD06_06700"
     CDS             complement(1399..2199)
                     /locus_tag="VFD06_06700"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HZN03262.1"
                     /translation="MRRIDLNCDVGEIPDLIADGTDDRLLARVTSANIACGGHAGDDA
                     TMRATLATALRHGCAVGAHPGYDDPAHFGRRRLDLPLDRVTGLVRDQVARLLGIARDT
                     GAALVHVKPHGALYNAAADDRDLAAAIAAGVRAACTDLGRPETPVPGGGLPILVGLAG
                     SASLEVYARSGFRVAAEAFADRRYEPDGRLRSRALSGALLEAPEEAAAQALAIVRDRR
                     VTASDGSHLEVAADTLCVHGDTPGAVRIAAAVRDALEAAGITVAPLPH"
     gene            complement(2242..3501)
                     /locus_tag="VFD06_06705"
     CDS             complement(2242..3501)
                     /locus_tag="VFD06_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN03263.1"
                     /translation="MSQRVRALVAAFLGWMLDGMDIMLYAFALTALQREFGLAPAALG
                     GVASITLVASAVGGIAAGALADRFGRARVLVWSILVYSFCTAGTATATGLTALLFWRA
                     LVGLGLGAEWSAGSVLVAETWPSEHRGKAIGFVQSGWAIGYLAAAGLAALILPRYGWR
                     ALFLVGVLPALLTVWIRRRVEEPEIWLRRQAGHTAAAASGAGPSGPPAGEPVLTTAQR
                     FRALLRPPLRRPMIVATTMGSALLFAYWGLFTWIPTYLSSPIEQGGAGLGIVKSSAWI
                     APMQIGAFLGYNLFGFIADRVGRRPTFLAFVLGAAAIVPLYGLASRHPGVLLALGPLV
                     GFFGHGYFSLFGAMLAELFPADVRGTAQGLCYNAGRAVSALAPWIIGAAAQSLGYGVA
                     LALTSMLYLVGGALIFLLPETRGRALT"
     gene            complement(3498..4484)
                     /locus_tag="VFD06_06710"
     CDS             complement(3498..4484)
                     /locus_tag="VFD06_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HZN03264.1"
                     /translation="MIGIDVLEPGLQTTVQDRGRPGHARFGVSAAGAADPLALDLGNR
                     LVGNPPEAAAVEMTLLGGSFRFEAPAVVALAGADMGAEADGLPFPPFASRRIRAGAIV
                     RCGAAAKGARCYLAVAGGIDTPPWLGSRSTHLPSRLGGLEGRALRRGDRLPIGALPAA
                     DPGVSSAAGGPPPMILRDRELLAPSRTIRVTAGAHAPRFDAETLRLFETAEFTVSATS
                     DRMGLRLDGATLPARGSGRMPSEGMPLGAIQVPPDGAPILLFVDHQTTGGYPVIAAVI
                     AADLWRVGQLRPGARLRFEPVTMETARALQLAQRQRLRSPDLLAADSAGPGA"
     gene            complement(4516..5241)
                     /gene="pxpB"
                     /locus_tag="VFD06_06715"
     CDS             complement(4516..5241)
                     /gene="pxpB"
                     /locus_tag="VFD06_06715"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HZN03265.1"
                     /translation="MRRRAAHRNRKRSGKDSPTPQSPSSRLIAASDTSLLVAFASGEE
                     GAVTAEERRCVRRLLRLLDASPLDGILDASPGDGSLLVRFDPERLDHRALGGHVERLL
                     PRCEEAVEPPPRRVTIPVRYGGEAGPDLEEVARLTRLDPDEVVRRHSAAVYEVRFLGF
                     TPGFAYLGTLDPSLVCPRLDRPRRAVPAGSVAIAADQTAVYPSATPGGWRIIGRTETR
                     MFDPGREPMSLLAIGDTVQFEPR"
     gene            complement(5210..5752)
                     /locus_tag="VFD06_06720"
     CDS             complement(5210..5752)
                     /locus_tag="VFD06_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03266.1"
                     /translation="MGGRLPRSLVTFAATLLFASSALAAAPLPRPATVGFFALRLARN
                     LGFEAKTAGEAQVLLSAAGVTFAGRLAEPLTEDEATRLLGDLGLETSPSADPDRILSQ
                     PFADRLAGIAARGVLATGGVPATEGTLPTSCQSLERSQCFQCCIASLGRLASVPQRMI
                     DLCNSSCTTLGAPPSSTSQP"
BASE COUNT          901 a         2300 c         2306 g          817 t
ORIGIN      
        1 gacggcgcgc tcctgtcggc cgcctggatc ggcgtcgcgc tgtcgctcgt cgtcctctgc
       61 gggtacgcca acgccctgat cctggcggcc ctgtgggccc tctacttctc cttcgtctcc
      121 atcgggcagg actggtacgg ctacgggtgg gagatccagc tcctggagac gggggccgtg
      181 gcgattctcc tctgtccgct cctcgacggc cggccgttcc cgcgccggcc gccgccgttc
      241 gcggcgatcc tgctgctccg gtggctggcg ttccgcatct acctcggggc ggggctgatc
      301 aagatccggg gcgacgcctg ctggcgcgat ctcacctgcc tcgtgcacca ctacgagacg
      361 cagccgattc cgaatccggt gagccgcttc tatcacttca tgccgctctg gttccacaag
      421 gggggcgtgc tttacaacca cctcatcgag ctcgccgccc cgtggtttct cttcgggccg
      481 cgcctcctgc gcctgacggc gggatcgctc atggtcgcct tccaggtcat cctgatgacc
      541 agcggcaacc tgtcgttcct gaactacctg acgatcgtcc cgtgcctcgc ggcgttcgac
      601 gacgccttcc tccggcgcct gctgccgcgc gtcgtgacgc ggagcgccga cgccgcggcg
      661 gccgcccacg ccgccgctgc gacgacggtg gcccacgatg ccgccgctgc cgcgacggca
      721 gggcctgccc ccgaagcgat cggggcgccg ccgcaggcgg ccctgttgaa gccctccggc
      781 gccggccgcg cgcacgaggc gatcgcctgg gcctatgccg cgctcgtcgc ggtgttgagc
      841 ctcggtccgg tcggcaacat gctgtcgagc cggcagatca tgaacacgtc cttcgaccgg
      901 ctgcacctgg tcaacaccta cggcgccttc ggcagcgtcg gcgaggtgcg tcgcgagatc
      961 gtcttcgagg ggaccgcgga cgacgccgtc accgccgcga ccgtgtggaa ggagtacgac
     1021 ttctggtgca agccggggac gccgacgcgc cgtccctgcc tcatcgcgcc gttccagccg
     1081 cgcctcgact ggcagatctg gttcgcggcg atgtccggtc cggagaggta cccctggacg
     1141 ctccacctgg tcgcgaagct cctgcgcaac gacgcggggg cgctgtcgct cctctcaacg
     1201 aacccgttcc cggaccggcc gccccggttc atccgcgccg cctattaccg ctaccagttc
     1261 gccccgccgg gggagccgga cggggcgtgg tggcgccgga cctatctcgg ggagtggctc
     1321 ccgccgctcg ccctcgagga cccgaggctg atccgtttcc tgcaggccta cggatggtga
     1381 ccgcgggcga cgcggcggtc agtgcggaag cggcgcgacc gtgatgccgg cggcctcgag
     1441 cgcgtctcgc accgccgcgg cgatgcggac cgctccgggg gtgtcgccgt ggacgcacag
     1501 cgtgtcggcc gccacctcga ggtgcgagcc gtccgacgcg gtcacgcgcc gatcgcgcac
     1561 gatggccagc gcctgcgcgg ccgcctcctc cggcgcttcg agaagggctc cggaaagggc
     1621 gcgggatcgc agccggccgt ccggctcgta gcgccggtcg gcgaacgcct cggccgccac
     1681 ccggaagccg ctccgcgcgt agacctcgag tgacgccgag ccggcgagtc ccaccaggat
     1741 cggcaggcct ccgcccggca ccggcgtctc cggccgcccg aggtcggtgc aggcggcgcg
     1801 cacccccgcg gcgatggcgg cggcgagatc gcggtcgtcc gccgccgcgt tgtacagcgc
     1861 gccgtgcggc ttgacgtgta cgagtgccgc tcccgtgtcc cgcgcgatcc cgaggaggcg
     1921 cgccacctga tcgcgcacca gccccgtgac ccggtcgagc ggcaggtcga ggcgcctcct
     1981 tccgaagtgc gcggggtcgt cgtagccggg atgggcgccg acggcacagc cgtgccggag
     2041 ggccgtggcc agggtcgcgc gcatcgtggc gtcgtcgccg gcgtggccgc cgcaggcgat
     2101 gttggccgag gtgacccggg ccaggagccg atcgtccgtg ccgtcggcga tgaggtccgg
     2161 gatctccccg acgtcgcagt tcaggtcgat gcgtcgaatg cgccggctcc tttttccgac
     2221 gcgcgggcgg ctcggcgcct cttacgtgag cgcgcgcccg cgcgtttccg gaagcaggaa
     2281 gatgagggcg ccgccgacaa gatacagcat cgacgtgagg gcgagggcga cgccgtagcc
     2341 gaggctctgg gcggcggcgc cgatgatcca cggcgcgagc gccgagacag cgcggccggc
     2401 gttgtagcag agcccctgcg cggtgccgcg cacgtcggcc gggaacagct ccgccagcat
     2461 cgcgccgaac aggctgaagt agccgtgccc gaagaagccg accagcggcc ccagcgcgag
     2521 gaggacgccg ggatggcgcg acgcgaggcc gtagagagga acgatcgccg ccgccccgag
     2581 gacgaacgcg aggaaggtcg gacgccgtcc gacccggtcg gcgatgaacc cgaacaggtt
     2641 gtatccgagg aaggcgccga tctgcatcgg cgcgatccag gcggaggact tgacgatccc
     2701 gagcccggcg cccccctgct cgatcgggct gctcaggtac gtcgggatcc aggtgaacag
     2761 cccccagtag gcgaagagaa gcgccgaccc catggtggtg gcgacgatca tgggacgccg
     2821 cagcggcggc cggaggagcg cccggaaccg ttgcgcggtc gtgaggaccg gctcgcccgc
     2881 cggcgggccg gacggcccgg ccccgctggc ggctgcggca gtgtggcccg cctggcggcg
     2941 gagccagatc tccggctcct cgacgcggcg gcggatccag acggtcagaa gcgccggcag
     3001 cacccccacg aggaagagcg cccgccagcc gtaccgcggc aggatcagcg cggcgaggcc
     3061 ggcggcggcg agatagccga tcgcccagcc ggactgcacg aagccgatcg ccttgccgcg
     3121 atgctccgag ggccatgtct ccgccaccag cacggagccg gccgaccatt ccgcgccgag
     3181 tccgagcccg acgagagccc gccagaagag cagggccgtg aggccggtgg ccgtcgcggt
     3241 gccggcggtg cagaacgagt agacgaggat cgaccacacg agcacgcgcg cccgcccgaa
     3301 gcgatcggcg agcgcccccg cggcgatgcc gccgacggca gaggcgacga gcgtgatcga
     3361 ggcgacgccg ccgagcgccg ccggagccag gccgaactcc cgctgcagcg cggtcagggc
     3421 gaaggcatag agcatgatgt ccatgccgtc gagcatccat ccgagaaacg cggcgacgag
     3481 ggccctgacg cgctggctca tgcgccgggc ccggcgctgt cggcggcgag cagatcgggg
     3541 gagcggagcc tctgccgctg cgcgagttgc agggcgcgcg ccgtctccat cgtcacgggc
     3601 tcgaagcgga ggcgcgcccc ggggcgcagc tgtccgacgc gccacagatc ggccgcgatc
     3661 accgcggcga tcaccgggta gcccccggtc gtctggtgat cgacgaacag caggatcggc
     3721 gcaccgtcgg gaggcacctg gatcgcgccg agaggcatcc cctcactcgg catccgtccg
     3781 gagccccggg ccgggagcgt cgcgccgtcg aggcggagcc ccatgcgatc ggaggtggcg
     3841 ctgacggtga actccgcggt ctcgaacagc ctcagcgtct ccgcgtcgaa gcgcggggcg
     3901 tgcgccccgg cggtgacccg gatcgtccgg gacggagcga gaagctcgcg gtcgcgcagg
     3961 atcatggggg gcgggccgcc ggcggcggac gagacgcccg ggtccgcagc cggcagcgcg
     4021 ccgatcggca gccggtcacc acgccggagc gcgcgcccct cgagaccgcc gagccggctc
     4081 ggaaggtgcg tcgaacggct gccgagccac ggcggcgtgt cgatcccccc cgcgaccgcg
     4141 agatagcacc tggcgccttt ggccgcggcg ccgcaccgga cgatcgcgcc ggcgcggatc
     4201 cgcctcgagg cgaagggggg gaaggggaga ccgtccgcct cggcgcccat gtcggctccc
     4261 gcgagggcga cgacggcagg cgcctcgaac cggaacgatc cgccgaggag ggtcatctcc
     4321 accgccgccg cctccggagg attcccgacg aggcggttgc cgagatccag cgcgagggga
     4381 tcggcggcgc cggccgcgga gacgccgaag cgcgcgtgcc ccggtcgccc gcggtcctgc
     4441 accgtcgtct gcagcccggg ctcgagcacg tcgatgccga tcacgcgccg cctcgcgatc
     4501 cccgccgacg ggtcctcacc gcggctcgaa ctgcaccgtg tcgccgatgg cgaggaggct
     4561 catcggctcg cgcccgggat cgaacatccg cgtctccgtt cggccgatga tccgccaccc
     4621 gcccggggtc gcggagggat agacggcggt ctggtcggcg gcgatggcga cgctgccggc
     4681 gggcacggcg cgccgcgggc gatcgaggcg cggacagacg agcgagggat cgagcgtgcc
     4741 gaggtaggcg aatcccggcg tgaagccgag gaaccgcact tcgtaaaccg cggcgctgtg
     4801 gcgccgcacc acttcgtccg ggtccagccg cgtgagccgc gccacctcct cgaggtcggg
     4861 gccggcttcg cctccatagc gcaccgggat cgtcacgcgc cgcggcgggg gctcgaccgc
     4921 ctcctcgcac cggggcagga gccgctcgac gtggccgccg agcgcccggt gatccaggcg
     4981 ctccggatcg aaacggacga gaagcgatcc gtcccccggg ctggcgtcga ggatcccgtc
     5041 caggggcgag gcgtcgagca gccgcagaag acgccgcacg cagcggcgct cctccgccgt
     5101 caccgcccct tcttcgccgg aggcgaacgc gacgagcaga ctcgtgtcgc tggccgcgat
     5161 cagacgggac gacggggact gcggcgtcgg actgtcctta ccggaacgct tacggttgcg
     5221 atgtgctgct cggcggcgca ccgagcgtcg tgcacgagct gttgcacagg tcgatcatcc
     5281 tctgcgggac gctcgcgagc cgaccgagcg atgcgatgca gcactggaag cactggctgc
     5341 gttcgagcga ctggcacgag gtcggaagcg tcccctcggt cgccggaact cctcccgtcg
     5401 ccaggacgcc gcgggccgcg atccccgcca ggcgatcggc gaacggctgc gacaggatgc
     5461 gatcgggatc ggcggagggc gaggtctcga gcccgaggtc gccgagcagg cgcgtcgctt
     5521 cgtcctcggt gagcggctcg gcgagcctcc ccgcgaacgt cacgccggcc gccgacagta
     5581 gcacctgcgc ttcgcccgcg gtcttcgcct cgaacccgag gttgcgcgcg aggcgcagcg
     5641 cgaagaagcc gaccgtcgcc ggtcgaggca atggtgcggc ggcgagtgcc gatgacgcga
     5701 aaagcagcgt ggcggcgaat gtcacgagac ttcgtggcag gcgacccccc atctccacct
     5761 cccgtggact acgtactacc gtcagggctc gtactaggag cctgtccgag tattcgcatg
     5821 ggtcgcgttc gcggcgtcgc ggggtcggtc gagaggcgcc gcgacgccgt cgatgctagg
     5881 ccatcgtcaa ggagcggcaa cgacgcgggc ggcccggcgc cgccgcgaac ccgaagggtg
     5941 catggggatg gcggtcccat gcttcgttgc tcgtcgtccg cgatccaacc acatcgcgga
     6001 atctaggcgc ctcgcctgca actcgcaagt cccctgcgcg cgacccatgc caatactcgg
     6061 acaggctcct caggtccaga ccgcccacgg gcggcgagga gcatcgcgca tccccgaaaa
     6121 ccgcgcggag tctacaaaag gaacttctgt gccgtcaaca ggttgcccca gacgtgggat
     6181 aatcgcgccg atgtccctcg gagttttcca tccgctcgtc cgcgactggt tcgcctcgag
     6241 gttcgccgcc ccgaccggag tgcaggaacg gggctggcac gccatcgccg ccggccggca
     6301 cgcgctcctg accgcgccga ccgg
//
LOCUS       DASRIQ010000274         6170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_365050, whole genome shotgun sequence.
ACCESSION   DASRIQ010000274 DASRIQ010000000
VERSION     DASRIQ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365050"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFD06_06725"
     CDS             <1..748
                     /locus_tag="VFD06_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03267.1"
                     /translation="FPVDYPVWLDRDDVARGRDTVLETALRWIGNRPPTASIRADSTT
                     ECTGPDGAGVRLDASTSTDPDSTTGTRDDIALFEWFEDFGAPTEQALGTGELIEPVLA
                     IGSHRVTLRVRDRFGEVALAETEFKVVDTMAPTMRIDVSPSILWPPDHRLVPVHAAVA
                     AEDRCGEARVVLTSVRSDQPDDGIGSGDGHTVDDVQDADPGREDFDVLLRAELGRARG
                     SMRTYSLIYTVSDPAGNSASASADVRVQAP"
     gene            853..1236
                     /locus_tag="VFD06_06730"
     CDS             853..1236
                     /locus_tag="VFD06_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN03268.1"
                     /translation="MLKNSPMYSYIPVKEIARARAFYEEKLGFKPKQLTKGGVVYEFA
                     GGTACFLYPTPNAGTSRASQAFWEVADIEQEVAELKARGVKFEKYDVPGMPVVDENGI
                     ATGGGAKAAWFKDTEGNIMAVIQSL"
     gene            1335..1559
                     /locus_tag="VFD06_06735"
     CDS             1335..1559
                     /locus_tag="VFD06_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03269.1"
                     /translation="MSYDVLSTLDPLDFMSGASCVASDVVALTCADTSTPALGQALFY
                     LVRPRNACPQGQGPLGDASNGTERVGRSCP"
     gene            1637..1834
                     /locus_tag="VFD06_06740"
     CDS             1637..1834
                     /locus_tag="VFD06_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03270.1"
                     /translation="MLGFCVASVYGILQALHVVFGRFYALLAVCAIRGMSLHAVDLWR
                     RFDDMKLSAKSPAGRVELRVD"
     gene            2030..5335
                     /locus_tag="VFD06_06745"
     CDS             2030..5335
                     /locus_tag="VFD06_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03271.1"
                     /translation="MTPRPRPRTHHDAAWFVRTLPRVVAVLLIAAAASWTAAMSAPEE
                     LLYTPATSPEPDRSVDLLGEASGWVLPNETTLDSFDAIESADRGTADNLCEGINGILT
                     GADPGWVRVGGSNDPFTSFTEARGQVLPDFNFKTNPFVNHTDAPFTHYAHDLNVFVTL
                     DAGYRHLLASGNFELGDPSEFGTLELEWERGGIPMFAWPSPHDRITVWGPWVFDCGHG
                     EPDENDSDVALSDAKYRTEIHSPVGWVVYRNTSNMTDRDGTPRPEKRMANPWTWYEAD
                     DFPGMGVPLPGMGGTLDTPIKATVADAFFSSFAGNIPEGLNGCDDTTVVSDDTIDSTC
                     FYTLSAETFEWAAPLLQQDYSFFIPAPPKPASDPDATLVWDSEDHCAQVPSNPANPPG
                     DDVEDVFEADDGAENIGSPACNIPDEVFPGEIVAGQIGIRVVVRASTGGVDYPANKYI
                     AFAKRYKVGWDHVDPDAQRPHSYQVDFQTLKVIDDTDSDTGGDGEWVVSLRANDKWIH
                     PVRGHGDDDEPFWEGGALAPDTDYSIGQSLTVTPLAGQPVRVWMKGWDDDNAPITHNN
                     NFNDILPTVNEYHLLADHRLPTPGTPLSFAVDGTPSFLGDEWEGGGAYKLSYTITDVT
                     DPQAPDCCILYGQPKYGPNADTGGLLRVNGTDAHETPIFLVNAQADGLEWRSWRVADA
                     IPAGWSFDLDASDQLRIELFGLASDSGTYNVQSAPVLTTDSRQIVSPRTRVQLQLDNT
                     PPALVVPGPVAVFADGTAGAHVDYTVGVSDNFPGPIDYACVPPSGALFPNGKNAPQTT
                     TVTCTATDTVENQSVETFDVTVTSPFGYLKDFVALGLEWTKLAGGVVVQSGNVGAFDS
                     SAGVPNNPGFEVVTASSTNLGSASTAACPSSSPPNAPQIAAESEKLGNLSSSGDVFFV
                     TPISAGNGSQHNPCPGYVPLFLGLPIAPPNGPGGADIQLSGSQTLGPGAYGKLSVKPN
                     AVVTFTGGSYTFAAIEVKPGATVTFAAPSTVVVAGRVLIADGGRIGPSSSGGFASRDI
                     VLYVNGADGPPNNPSDAFSAGTGAVVRVNAFVPNGTLSMGTSTAGTGAFIGRRVSFGT
                     SVVLALDSSFIVP"
     gene            5491..>6170
                     /locus_tag="VFD06_06750"
     CDS             5491..>6170
                     /locus_tag="VFD06_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03272.1"
                     /translation="MSASDSGRWSATMLSFVILALVGGFPTAIAGVATHPVPLQNSDC
                     AGAGGTPGFTGHALFEYRIETTGAVQGIKLLYAEVQPETRKPGFLAEVTGCLEKWKFR
                     PATVDGVPAAAVMKVAFHRLPPAPAGGDEVMLPGGKIVPASLLKQVRAATLTFTESLL
                     KGPAYREAKGNGWLIRTDLPKASLDDMQAAIDFARRVFDEAFPGPGQGEASQDVTLIL
                     FKDEEKYR"
BASE COUNT         1026 a         2143 c         2011 g          990 t
ORIGIN      
        1 attccccgtg gactatcccg tctggctgga ccgcgacgac gtcgcccgcg gcagggacac
       61 ggtgctggag acggcccttc gctggatcgg caatcgcccg cccacagcct ccatccgcgc
      121 cgattccacg acggagtgca cggggcctga cggggccggg gtccgtctgg atgcctcgac
      181 ctcgaccgac cccgactcga ccacgggcac ccgcgacgac atcgcgctct tcgagtggtt
      241 cgaggacttc ggcgcaccga cggagcaggc gctcggcacc ggggagctca tcgagcccgt
      301 cctggcgatc ggatcccatc gcgtcactct gcgcgtgcgg gacaggttcg gcgaggtcgc
      361 cctcgcggag accgaattca aggtcgtcga cacgatggct ccgacgatgc ggatcgacgt
      421 ctctccttcc atcctgtggc cccccgacca ccgtctcgtc cccgtgcacg ccgccgtggc
      481 agcggaggat cggtgcggcg aagcgcgcgt ggtgctgacc tccgtccgat ccgatcagcc
      541 cgacgacggg atcggatcgg gggacggaca tacggtggac gacgtccagg atgccgaccc
      601 gggacgggag gacttcgacg tcctcctccg cgccgagctc ggacgcgccc gcggcagcat
      661 gcgcacgtac tcgctgatct acacggtgag cgacccggcg gggaattccg cctccgccag
      721 cgccgacgtg cgcgtgcagg cgccgtagcc ggaaccgacg ctcgcgctga agtccttggc
      781 gcgctcgctg accgggtcta tcttgggggt cgagaccggc cgcgctcttt cgcggcaagc
      841 aaggaggacc ccatgctgaa aaactctccg atgtattcct acataccggt gaaggaaatc
      901 gcccgggcac gcgcgttcta tgaagagaag ctgggtttca agcccaagca gctcacgaaa
      961 ggcggcgtcg tctacgagtt tgccggcggc accgcgtgct tcctctatcc gacgccgaac
     1021 gccggcacgt cccgggcaag tcaggccttc tgggaggtcg cggacatcga acaggaggtc
     1081 gcggagctga aggcgcgcgg cgtgaagttc gagaagtacg acgtgccggg catgccggtc
     1141 gtggacgaaa acggcatcgc gaccggcggc ggcgccaagg ctgcgtggtt caaggatacg
     1201 gaaggaaaca tcatggcggt gatccagtcc ctctgaccct gcctatctcg tcaccgtggc
     1261 ccagaagggg aggaactact tcgcgtcctt gatcccctgg tccgtgacgc cctttgcagc
     1321 cgggaggact gaacgtgtcc tacgacgtcc tgtccacgct tgacccgctc gatttcatgt
     1381 cgggggcgtc gtgcgtggcc tccgatgtcg tggccctgac ctgtgccgac acgtccactc
     1441 cggccctcgg ccaggcactg ttctacctgg tccggccgag gaatgcctgc cctcagggcc
     1501 aggggccgct cggcgacgct tcgaacggca ccgagcgggt cggtcgctca tgtccctgat
     1561 cgtgaggact cgatgcggcg cgcaattcgt atgcaggttc atatgatttc tgcgggtccg
     1621 ccccacttct gaggcgttgc tcggcttctg cgtggcgtcc gtctacggga tcctccaggc
     1681 cctgcacgtg gtgttcgggc gcttctacgc gttgctggcg gtgtgtgcca tccgcggaat
     1741 gtcgctgcat gcggtcgacc tgtggcgccg gtttgatgac atgaagctca gcgccaaatc
     1801 gccggccgga cgcgtcgagc tccgcgtgga ttgacgcgcc gcccgcggct tgggcggtcg
     1861 acatgtccgg atttccccgc agtccaggcg gattctgtca gggtccaccc gacgcgcatt
     1921 gacagagatt caggggccgt gctatagcct gcgccaacct cactctttcg cttttcgctt
     1981 tggcgccgaa tcgactggag ccggagattc cggctaagga gcctgtgcca tgacgccccg
     2041 tccgcgaccc cgaacccacc acgatgccgc ctggttcgtc cgcacgctcc cgcgcgtcgt
     2101 ggcggtgctg ctgatcgcgg ctgccgcctc gtggaccgcc gcgatgtccg cgcccgagga
     2161 actcctctac acgcccgcca cgtcccccga accggacagg agcgtggatc tcctcggaga
     2221 ggcgagcggc tgggtccttc cgaacgagac gacgctcgac agcttcgacg cgatcgaatc
     2281 ggcggaccgg ggcacggcgg acaacctgtg cgaggggatc aacggcatcc tcaccggggc
     2341 cgatccgggc tgggtgcgcg tcgggggcag caacgaccca ttcacgtcct tcaccgaggc
     2401 gcgcggacaa gtgttgccgg acttcaactt caagaccaac ccgttcgtca atcacaccga
     2461 cgcgccgttc acccactacg cccacgacct gaacgtcttc gtgaccctcg atgccgggta
     2521 tcgccacctg ctcgcgagcg gcaacttcga gctgggtgat ccgagcgagt tcggcacgct
     2581 ggagctcgaa tgggagcgtg gcgggatccc gatgttcgcc tggccctcgc cgcacgaccg
     2641 catcacggtc tggggtccgt gggtgttcga ctgcggccac ggggagcccg atgagaacga
     2701 ctccgacgtc gccctcagcg atgcgaagta ccgcaccgag atccactcgc cggtgggctg
     2761 ggtcgtgtac cgcaacacct cgaacatgac cgatcgcgat ggcacgccgc gcccggagaa
     2821 gcggatggcc aatccctgga cctggtacga ggcggacgac ttccccggca tgggcgtccc
     2881 cctgccgggg atgggcggca cgctggacac ccccatcaag gcgacggtgg ccgacgcctt
     2941 cttctccagc ttcgccggaa acatcccgga gggcctgaac ggctgcgacg acacgacggt
     3001 cgtcagcgac gacaccatcg actcgacctg cttctacact ctcagcgcgg aaacgttcga
     3061 atgggcggcg cctctcctgc agcaggacta cagcttcttc atcccggcgc ccccgaagcc
     3121 ggcgtccgat cccgacgcga cgctggtgtg ggactccgag gaccattgcg cccaggtccc
     3181 gagcaacccc gccaatcctc ccggagacga cgtcgaggac gtgttcgagg cggacgacgg
     3241 cgccgagaac atcggcagcc ccgcgtgcaa cattcccgac gaggtcttcc cgggggagat
     3301 cgtggccgga cagatcggca tccgcgtggt cgtcagggct tcgaccggcg gggtggacta
     3361 tcccgccaac aagtacatcg ccttcgcgaa gcgctacaag gtgggatggg atcacgtcga
     3421 tcccgatgcg cagcgccccc acagctatca ggtcgacttc cagacgctca aggtgatcga
     3481 cgacacggac agcgacaccg gcggcgacgg cgagtgggtg gtgtcgctgc gggcaaacga
     3541 caagtggatc catcccgtgc gcgggcatgg cgacgacgac gagccgttct gggaaggcgg
     3601 ggccctcgcg cccgacaccg attacagcat cggccagagc ctcaccgtca caccgctcgc
     3661 gggccagccg gtccgcgtct ggatgaaggg gtgggacgac gacaacgccc ccatcaccca
     3721 caacaacaac ttcaacgaca tcctcccgac ggtcaacgag tatcacctgc tggcggatca
     3781 tcgcctgccg actcccggta ccccgctctc gttcgccgtc gacggcacgc ccagcttcct
     3841 gggggacgaa tgggaggggg gcggggccta caagctgtcc tacacgatca ccgacgtcac
     3901 cgacccgcag gctcccgatt gctgcatcct ctatggccag ccgaagtacg gacccaacgc
     3961 cgacaccggc ggcctgctgc gcgtcaacgg caccgatgcc cacgagacgc ccatctttct
     4021 ggtcaacgcc caggccgacg gcctcgaatg gcggtcctgg cgggtcgccg atgcgatccc
     4081 ggcgggctgg agcttcgacc tcgacgcgtc ggaccagctg cgcatcgagc tcttcggcct
     4141 cgcgagcgac agcgggacgt acaacgtgca gtcggcaccc gttctgacca cggacagtcg
     4201 ccagatcgtg tcgccgcgga cgcgcgtgca gctgcagctc gacaacacgc ctcccgcgct
     4261 ggtcgtgccg ggccccgtcg ccgtcttcgc ggacggcacc gcgggggccc acgtggacta
     4321 cacggtcggc gtgagcgaca acttccccgg tccgatcgac tacgcctgtg ttcctccgtc
     4381 gggcgcactc ttcccgaacg gcaagaatgc tcctcagacc accacggtga cctgcacggc
     4441 caccgacacg gtggagaacc agtcggtcga aaccttcgat gtcaccgtga cctccccctt
     4501 cggctacctc aaggacttcg tggcgctggg gctcgagtgg acgaagctgg ccggcggcgt
     4561 ggtggtccag tcgggcaacg tcggcgcctt cgactccagc gcgggcgtgc ccaacaaccc
     4621 cggcttcgag gtggtcaccg cctcgtcgac gaacctcgga tccgcatcga ccgcggcttg
     4681 cccctcgtcg agcccaccaa acgctccgca aatcgccgcg gagagcgaga agctgggcaa
     4741 tctcagttcc tcgggggatg tgttcttcgt gacgccgatc agtgccggca atggctcgca
     4801 gcacaaccca tgcccgggct acgtgccgct tttcctgggt ctgccgatcg cccctcccaa
     4861 tggtcccgga ggggcggaca tccagctgtc ggggagccag acgctcgggc ccggcgccta
     4921 cggcaagctg agcgtgaagc cgaacgccgt cgtcacgttc accggcggca gctacacctt
     4981 cgcggccatc gaggtgaagc ccggggcgac cgtgaccttc gcggcgccgt ccacggtcgt
     5041 cgtcgccggc cgtgttctga tcgccgatgg cggtcgcatc gggccgtcgt cgtcgggcgg
     5101 tttcgcgtcc agggacatcg tcctctacgt gaacggagcc gacggaccgc cgaacaatcc
     5161 gtccgacgcg ttcagcgccg gcaccggcgc cgtggtgcgg gtcaacgctt tcgtgccgaa
     5221 cggcaccctc tcgatgggga cttcgaccgc gggaacggga gccttcatcg gcaggagggt
     5281 gagcttcggc accagcgtgg tgctcgcgct ggacagttcg ttcatcgtcc cctgagcgaa
     5341 gatcgaccgt tcatcgccac gcggtggctc ctacagcgag gctcctccaa ccctcgcgga
     5401 aggtcaacgg cgacgttcac gtcctgtcct atcggcaacc ctgaaggccg acgcgattcc
     5461 gttcacgagc gaagcccgca ggagcgatgc atgagcgcat cggattccgg ccgctggtcg
     5521 gcgacaatgc tgtccttcgt catcctggct ctcgtaggag gcttccccac cgcgatcgcc
     5581 ggcgtcgcga cgcaccccgt gccgctgcag aactccgatt gcgccggggc gggcgggacc
     5641 cccggcttca ccggacacgc cctgttcgaa taccgcatcg agacgaccgg agccgtgcag
     5701 gggatcaagc tcctctatgc ggaggtccag ccggaaaccc ggaagccggg gttcctggcc
     5761 gaggtgacgg gctgcctcga gaagtggaaa ttccgcccgg caaccgtcga tggcgtcccg
     5821 gcggcggcgg tcatgaaggt ggcgttccac cggttgccgc ccgcacccgc gggaggagac
     5881 gaggtgatgc tgccgggcgg gaagatcgtc cccgcgtcgc ttctcaagca ggtccgggcc
     5941 gcgacgctga ccttcacgga atcgctgctc aagggaccgg cctacaggga agcgaagggg
     6001 aacgggtggc tcatccgcac cgacctgccc aaggcctccc tggacgacat gcaggcggcc
     6061 atcgacttcg ccaggcgcgt cttcgacgaa gcgtttcccg ggccgggaca gggggaagcc
     6121 tcgcaggacg tgaccttgat cctgttcaag gacgaggaaa agtaccgcca
//
LOCUS       DASRIQ010000275         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1678393, whole genome shotgun sequence.
ACCESSION   DASRIQ010000275 DASRIQ010000000
VERSION     DASRIQ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678393"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VFD06_06755"
     CDS             <1..1190
                     /locus_tag="VFD06_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009751372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HZN03273.1"
                     /translation="GRGACLRTVPACVGGVAPACIPGPPSFDVCNGIDDDCDGLVDNG
                     DADHDLSGVCVDCNDTVASMHPGAPELCNGVDDDCNGIADDRNGAVSDPDADGRAGAC
                     DNCPSIANPGQEDSDHDGLGDACDPCPADPAQFPDTDGDGTPDACDLCPLNPAPTTDV
                     DGDGIGAACDNCPTVANPGQQDGDLDGFGDACDACPLRATPTNDPTDADGDGIASACD
                     NCPLAPNPGQQDFDHDGFGDACDSCPLLATPPQDQSDGDGDGVLDACDNCVFNVNTDQ
                     SDTDGDGEGDACDLNDGLLMVWVTGPEEVDWDSEQTFLLYDVYRGDLDRLKATGESTQ
                     DPAVVPLAGRFCGMSEPFLLDEPPPPGKAVFYLVAATTTTGYQGIGSDSSGHPRLDLH
                     PCP"
     gene            1269..2321
                     /gene="hrcA"
                     /locus_tag="VFD06_06760"
     CDS             1269..2321
                     /gene="hrcA"
                     /locus_tag="VFD06_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZN03274.1"
                     /translation="MSAKESELPPRAQEILRCVVSHYILTGEPVGSRTIAKMSWEGLS
                     PASIRNVMSDLEEAGFLVQPHTSAGRVPTDKGYRYYVDTLLPAGRLAPQDRALIDQSL
                     RDLAGEPGSTMEAIPKLLSRLTRQVAYLLAPPPADAVLKHIEFVRLHPKRVLVVFVDR
                     NEVVSHRLLDTDEDYDAQVLEKAGRYLVAEFAGRALGEIRRRLVGLMAEEKARFDTLM
                     RDAVALGTRYFDAEAGARRIVVEGTTNIIHHPELADVEIMRGLFATFEEKHRLVTLLD
                     RYLDADRSRIVIGSEAGDLASGRLSLVASPYQAGEGGTGWIGVLGPTRMDYDRALALV
                     EYISRLFSTLLRRPIP"
     gene            2347..3102
                     /locus_tag="VFD06_06765"
     CDS             2347..3102
                     /locus_tag="VFD06_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZN03275.1"
                     /translation="MSARPSGKSKERPQPGPSKDEETGPGCPLPEDGDIEILEVIGVN
                     ETEPVIGGETGAIAHLEHATHDDGEAHHGSHAHPHHGGDEAADLRHRAEEAEKEKERL
                     HDLWLRAQAETSNVRKRLDREATDRVASETAERIRRLLPILDSLERAIGSPGWGDDGL
                     RQGVALTLQQMLEVLGRDGLKPIGSSGTLFDPRFHEAVEMVPHSEAPEGTVLEEMQRG
                     YLLRDRLLRPALVKVARSAGAADGGAERRRSAG"
     gene            3118..>3908
                     /locus_tag="VFD06_06770"
     CDS             3118..>3908
                     /locus_tag="VFD06_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZN03276.1"
                     /translation="MGKVIGIDLGTTNCCVAIMEGGVPKVIPNKEGARTTPSVVAFTE
                     RGEKLVGQIARRQAVTNPKNTVYAVKRLIGRKYSSPEVQHARQFVPYQISEAANGDVR
                     VRVREKDYSPQEISAMILGRLKEMSEEFLQIDVSEAIITVPAYFDDSQRQATKDAGRI
                     AGLKVLRILNEPTAASLAYRLNETSEKKIAVYDLGGGTFDVSILQLGQGVFEVRATSG
                     DTFLGGEDFDQRILEWLLQEFERKEGISLREDRLALQRLKEASEKA"
BASE COUNT          652 a         1335 c         1365 g          556 t
ORIGIN      
        1 gcgggcgcgg cgcctgcctg cggaccgtgc ccgcctgtgt cggaggggtg gccccggcgt
       61 gcatccccgg gccgccgtcg ttcgacgtct gcaacgggat cgacgacgac tgtgacggcc
      121 tcgtggacaa cggcgacgcc gatcacgacc tttcgggcgt ctgtgtcgat tgcaatgaca
      181 ccgtcgcctc gatgcatccc ggggcgcctg aactctgcaa cggcgtcgac gacgactgca
      241 acggcatcgc ggacgatcgc aacggcgcag tgtccgaccc tgatgcggac ggccgcgccg
      301 gcgcgtgcga caactgcccg tcgatcgcca accccggcca ggaggattcg gatcacgatg
      361 ggctcgggga cgcctgtgac ccctgtcccg cggacccggc gcagttcccg gacaccgatg
      421 gggatggcac gccggacgcg tgcgatctct gtccgctcaa tcccgctccg acgaccgatg
      481 tcgatggaga cggcatcgga gcggcgtgcg acaactgtcc gacggtcgca aacccgggtc
      541 agcaggacgg cgatctcgac ggattcggag acgcgtgcga tgcatgcccc ctccgcgcga
      601 caccgaccaa cgatccgacc gatgccgacg gagacggcat cgcctccgcc tgcgacaact
      661 gccccttggc gccgaatccc ggccagcagg acttcgacca cgatggattc ggagacgcct
      721 gcgacagctg cccgttgctt gcgacaccac cgcaggacca gagcgacggg gacggagatg
      781 gcgttctcga cgcgtgcgac aactgtgtct tcaatgtcaa cacggaccaa tcggacaccg
      841 acggcgacgg ggaaggggac gcctgcgatc tgaacgacgg gctcctcatg gtctgggtca
      901 ccggtccgga agaggtcgat tgggactccg agcagacctt cctgctgtac gacgtctatc
      961 gcggggatct cgatcggctc aaggcgaccg gcgaatcgac gcaggatccc gcggtcgtcc
     1021 cgctcgccgg acggttctgc ggcatgtcgg aaccgttcct gctggacgag cccccgcccc
     1081 ccggcaaggc ggtcttctac ctggtcgcgg cgacgacgac cacgggttat caggggattg
     1141 gcagcgacag ctccgggcat ccgagactgg acctgcatcc ctgcccgtga tgcgcgcgca
     1201 tcggagaaca cgctcggacc tgcgatagac gggctttgcc gggcgggtta tattggcact
     1261 cgctgcttat gagtgccaaa gagtccgagc tgccgccccg cgcccaggaa atcctccgct
     1321 gcgtggtctc ccactacatc ttgacggggg agccggtcgg gtcgcggacc atcgcgaaga
     1381 tgtcctggga gggcttgagc ccggcctcga tccggaacgt catgtccgat ctggaggagg
     1441 cggggttcct cgttcagccg cacacctcgg ccgggcgggt gccgaccgac aagggctacc
     1501 ggtactacgt cgacacgctc ctgcctgccg ggcggctggc cccgcaggac cgggccctca
     1561 tcgatcagag cctgcgcgat ctcgccggcg agccgggttc gacgatggag gcgatcccga
     1621 agcttctgtc ccggctcacc cggcaggtcg cgtacctcct ggcgccgccg ccggcggatg
     1681 cggtcctcaa gcacatcgag ttcgtccgcc tccacccgaa gcgggtgctg gtggtcttcg
     1741 tcgatcgcaa cgaggtcgtg agccaccggc tgctcgacac cgacgaggac tacgacgccc
     1801 aggtcctgga gaaagcggga cgctatctcg tggcggagtt cgcggggcgg gcgctcggcg
     1861 agatccgccg gcgcctcgtc gggctgatgg cggaggagaa ggcccgcttc gacaccctga
     1921 tgcgcgacgc cgtcgccctc ggcacgcgct acttcgacgc cgaggcgggg gcgcggcgca
     1981 tcgtggtcga ggggacgacc aacatcatcc atcacccgga gctggccgac gtcgagatca
     2041 tgcgggggct gttcgccacc ttcgaggaga agcaccggct ggtgacgctc ctcgaccgct
     2101 acctcgacgc cgatcggtcc cggatcgtca tcggctcgga agcgggcgac ctcgcctccg
     2161 gccgcctgtc gctcgtcgcc tctccgtacc aggcggggga aggcgggacc ggctggatcg
     2221 gcgtgctcgg cccgacccgc atggactacg accgggcgct cgcgctcgtc gagtatatct
     2281 cccgtctttt ctccactttg ttgcgtcgcc cgatcccgta acctatattc cggcgccgga
     2341 ggaaccatgt ccgcgcgccc gtccggcaag tccaaagagc gcccgcagcc gggtccctcg
     2401 aaggacgagg agaccggccc gggctgcccc ctccccgaag acggcgacat cgagatcctc
     2461 gaggtcatcg gcgtcaacga gaccgagccg gtgatcggcg gagagacggg cgccatcgcg
     2521 cacctcgagc acgccaccca cgacgacggc gaggcgcatc acgggtcgca cgcgcatccg
     2581 caccatgggg gcgacgaggc ggccgatctg cgccatcgcg cggaagaagc ggagaaggaa
     2641 aaggagcgcc tgcacgacct gtggctgcgc gcccaggccg agacctcgaa cgtccgcaag
     2701 cggctggacc gcgaggcgac cgaccgcgtc gccagcgaga ccgccgaacg gatccggcgc
     2761 ctgctgccga tcctcgacag cctcgagcgg gcgatcgggt cgcccggttg gggagacgac
     2821 gggctgcgcc agggggtggc gctgacgctc cagcagatgc tcgaggtcct gggacgggac
     2881 ggcctgaaac cgatcggctc cagtgggacc ctcttcgacc cgcgcttcca cgaggcggtc
     2941 gagatggtgc cgcactcgga ggcgccggaa gggacggtcc tggaggagat gcagcgcggc
     3001 tacctgctgc gcgaccggtt gctccggccg gccctggtga aggtcgccag gtccgccggg
     3061 gcggcggacg gcggggcgga gcgccgccgc tccgcggggt aacggagggg agagacgatg
     3121 ggcaaggtca tcggcatcga cctcgggacg accaactgct gcgtggcgat catggagggg
     3181 ggcgtcccca aggtgatccc gaacaaggaa ggcgcccgca ccaccccttc ggtcgtggcg
     3241 ttcaccgagc gcggcgagaa gctggtcggg cagatcgccc gccgccaggc ggtcaccaac
     3301 cccaagaaca ccgtctacgc ggtcaagcgg ctgatcggcc gcaagtactc gagcccggag
     3361 gtgcagcacg cccgccagtt cgtcccgtac cagatcagcg aggcggcgaa cggcgacgtc
     3421 cgcgtgcggg tgcgggagaa ggactacagc ccgcaggaga tctccgcgat gatcctcggg
     3481 cgcctcaagg agatgtcgga ggagttcctg cagatcgacg tcagcgaggc gatcatcacc
     3541 gtcccggcct acttcgacga cagccagcgc caggcgacca aggacgccgg gcggatcgcc
     3601 gggctcaagg tgctgcgcat cctgaacgag ccgaccgccg cgtcgctcgc ctatcgcctg
     3661 aacgagacga gcgagaagaa gatcgccgtg tacgacctcg gcggcgggac gttcgacgtg
     3721 tcgatcctgc agctcggcca gggggtgttc gaggtgcgcg ccacctccgg cgacaccttc
     3781 ctcggcggcg aggacttcga ccagcggatt ctcgagtggc tcctgcagga gttcgagcgc
     3841 aaggagggga tttccctgcg cgaggaccgc ctggcgctgc agcgtctcaa ggaggcctcg
     3901 gagaaggc
//
LOCUS       DASRIQ010000276         6945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3781018, whole genome shotgun sequence.
ACCESSION   DASRIQ010000276 DASRIQ010000000
VERSION     DASRIQ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781018"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFD06_06775"
     CDS             <1..808
                     /locus_tag="VFD06_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZN03277.1"
                     /translation="KKTALAAVERSGRGPELRALEERYSANFAEKREAVRDLAAALER
                     RDDAAIAAGGASVRDLGARGEALRTEAKDLVRAVDPRENPNDADYVFLTFVIDHLPRG
                     IVGLLIAVIFCAALSANSSELNALASTSTVDVYRRLLRPAESERHYVRVSRVLTALWG
                     FLAIGFALGAGLVENLIEAVNILGSLFYGTILGLFLTAFLLKRVRGTAVFLAALAAEG
                     TVIVLYLTTTIGYLWFNVIGCALVLALGGLFEVLGPGPRKQAVAPAGTVR"
     gene            867..1733
                     /locus_tag="VFD06_06780"
     CDS             867..1733
                     /locus_tag="VFD06_06780"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HZN03278.1"
                     /translation="MKKALLFTAVLSLLLPAGAFAQQQLKLPRPSPKASVQQTVGLTE
                     VTINYCRPGVKGRTIWGDLVPYDKVWRTGANEATTIQFSQDVKVNGKPLPAGLYSVHT
                     IPSKSAWTVILNKKAEQWGSYEYDEKQDALRLQVQPTAGPHQEFMEFSFPEVTPEGAT
                     IELAWEKVRVPFKIEVATKEQAMANIRQALSGDVKEWTVPYSAANYSFTSNLGNADEA
                     MKWIDQSVGIKETYWNLRLKAAMLEKAGKKKEAVTVAEKAVQVGKANEDEPSEIAKTE
                     KQIAEWKKSIAG"
     gene            complement(1809..2102)
                     /locus_tag="VFD06_06785"
     CDS             complement(1809..2102)
                     /locus_tag="VFD06_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03279.1"
                     /translation="MVRDVFERLDNRLRRIGEVRLAIAVLEDAVRCIELGHATRNFQA
                     RVKRRDAERWIASHARTHVFAFENVCGLLKLSSEEIRARIACWRERSRAACHT"
     gene            complement(2309..2959)
                     /locus_tag="VFD06_06790"
     CDS             complement(2309..2959)
                     /locus_tag="VFD06_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN03280.1"
                     /translation="MTMRILVADDHEVVRHGVRALLEARPGWQVVAEAVDGREAVEKA
                     KRLQPDVIILDIGMPGLNGLDAARQILKAAPKSGILILTMHESEQVVREVLAAGARGY
                     VLKSDAGRDLVNAVEAVGRQRIFFTSSVAQVVLKRSLGRNPETTPPAEPVSLTHRERE
                     VVQLLGEGKSNKEVADILGISVRTAETHRSNIMHKLECGSLSDLVRYAIRNHIITV"
     gene            complement(2959..5184)
                     /locus_tag="VFD06_06795"
     CDS             complement(2959..5184)
                     /locus_tag="VFD06_06795"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HZN03281.1"
                     /translation="MDDARNDMPAPPGPPLRAEVEPSRWVGIVLLAAVYFLAARLGLK
                     LDAVGGFASPVWPPTGIAIAALGLFGTRLWPGIALGALAANFAAGAPALAACGMAAGN
                     TLEALFAIALMRGPQGLRASFDRIGETLRFVLLGAALAPVVSASFGVVSGYLGGVIPG
                     VSLGRAWASWWLGDFMGALVVAPLLLAWADRPPFRLTLPRAAEAAALCAAFAFLGRYV
                     FWGGTSPLVQEMLRPSALFPLLIWGALRFGRIGSVTAVAALSVLSIVPSAMGHGPFAH
                     TSLNESLLLAQVFMAVTAVTMLVFSAGLSERRQALRRIQVSYVAARALGEASSVEVAS
                     PEILRVICENFEWDCAHVWTVDHEAGVLRHGAHWHAPGDRLMLCARSAESMTFHPGVG
                     LAGRIWSEGRSIWIRSLQASYDLVRAETAAELGLKSGMGAPILLGQEVHGVLTFFSRK
                     TREPDASHQQLLTALGSQMGQFMERRRAESDLRAAHADLEARIERRTRQLSDANRALE
                     DEIGVRRQTEQALRQLSTRLLKVQDEERQRLARDLHDSTAQVLAALSMKLTVLQRGVS
                     RFDRRTRALADEGAALAELCVRQIRSMSYLLHPPLLEEMGLPAALRWCAEGFSQRSGI
                     AVEVDLPARFGRLPMEVENALYRIVQECLSNVQRHSGSPTARVQLTRTEDGVELEVRD
                     QGRGMPIAIGDDRGGIETLGLGLLGMRERVRQLGGQMTIRSTTQGSTVHVAIDVPRAE
                     A"
     gene            5356..>6945
                     /locus_tag="VFD06_06800"
     CDS             5356..>6945
                     /locus_tag="VFD06_06800"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HZN03282.1"
                     /translation="MALVLCAAGAGGAPGPPSGGPRVLSARTVVAAFLVDRFEARLLT
                     ADVKPRPYLEPPAPPGLRDAGEGGAAQVEIALRDAAGTRFTLRPEIRGLCLEHPPGEP
                     PHIEGDTIRLHRETFLVELPDLPGAGQVEIAIGERGRDHRAVRRLLGAAALDAAPAPG
                     PGGHGGSATEGFTPAQPPALATAGQVHWPEEFSDPDVTFTWGDVSEAAKRINIVLVPD
                     GYTYAEKGTLNAHAQALIDYLRTTTPYREHDRFINYTLVYAYSSQSGTDQCDCASVVD
                     TAMNTAFPSSGDPCGGSANRCLYYGSGCDTSSAVNIALAEARAPAHDTTVVLVNTTRY
                     GGCGGQRAVYSAGNSAATDIAAHELGHSLAGLADEYVSFAGCGGGAGEINTSTNGVNG
                     AWPEWIGDLGAPRLGAQYWSQCLYRPANDCKMRSLFTPFCPVCNQHWSLTVFGHPRVA
                     FSAPLTALVPPEQTLSMPVGGSQAFSVTTRLQTTPGVTNRITWTVQGPGDPAPVTVAT
                     GVTSYSRSFPSVGAYTVRCDVE"
BASE COUNT         1075 a         2447 c         2423 g         1000 t
ORIGIN      
        1 caagaagacg gctctcgcgg cggtggagcg gagcggccgg gggccggagc tgagggccct
       61 cgaggaacgc tactcggcca acttcgccga gaagcgggag gcggttcgcg acctggccgc
      121 ggcgctcgag cgccgggacg acgccgcgat cgcggcgggg ggcgcgagtg tccgtgacct
      181 cggtgcccgc ggcgaggcgc tgcggaccga ggcgaaggat ctcgtccggg cggtggaccc
      241 gcgtgagaat ccgaacgacg cggactacgt cttcctgacg ttcgtcatcg accatctgcc
      301 gcgggggatc gtcggcctcc tgatcgccgt catcttctgc gccgcgctgt cggcgaattc
      361 ctcggagctc aacgccctcg cctcgaccag caccgtggac gtctaccgcc gcctcctgcg
      421 gccggcggaa agcgagcgtc actacgtgcg ggtgtcgaga gtgctgaccg ccctctgggg
      481 attcctggcg atcggcttcg cgctcggcgc gggcctcgtc gagaacctga tcgaggcggt
      541 gaacatcctg gggtcgctct tctacgggac catcctcggc cttttcctga ccgcctttct
      601 cctgaagcgg gtgcgcggca cggcggtctt cctggcggcg ctcgccgcgg agggaacggt
      661 gatcgtcctg tacctgacga cgaccatcgg ctacctgtgg ttcaacgtca tcggctgcgc
      721 cctggtgctc gcgctggggg gcctgttcga ggtgctcggc cccggccccc ggaagcaagc
      781 ggttgctccg gcgggcacgg ttcgttagga tccggtcctc tctcacagag gaccattctc
      841 aaaggatgtc tcgaggaggt acgtgcatga aaaaagccct gctgttcact gccgtgttgt
      901 ccctgctgct gccggcgggc gccttcgccc agcagcagct caagctgccc cgcccgagtc
      961 cgaaggcctc ggtccagcag accgtcggcc tgaccgaggt caccatcaac tactgccgcc
     1021 ccggcgtgaa gggacggacc atctggggcg atctcgtccc ctacgacaag gtctggcgga
     1081 ccggcgccaa cgaggcgacc accatccagt tcagccagga cgtgaaggtc aacggcaagc
     1141 cgctgccggc ggggctgtac agcgtgcaca ccatcccgtc gaagagcgcc tggacggtga
     1201 tcctcaacaa gaaagcggag cagtggggct cctacgagta cgacgagaag caggacgcgc
     1261 tgcgcctcca ggtccagccg acggcggggc cgcaccagga gttcatggag ttctccttcc
     1321 cggaggtcac gccggagggg gccaccatcg agctggcctg ggaaaaggtg cgggtgccgt
     1381 tcaagatcga ggtcgccacc aaggagcagg cgatggcgaa catccgtcag gccctgtcgg
     1441 gcgacgtgaa ggaatggacc gtcccttaca gcgccgccaa ctactcgttc acctcgaacc
     1501 tgggtaacgc cgacgaggcg atgaagtgga tcgaccagtc ggtggggatt aaggagacct
     1561 actggaacct gcgtctcaag gccgcgatgc tcgagaaggc ggggaagaag aaggaggcgg
     1621 tgacggtcgc cgagaaggcg gtgcaggtcg gcaaggcgaa cgaggacgag ccgtcggaaa
     1681 tcgccaagac cgagaagcag atcgccgagt ggaagaagtc gatcgccggc tgagccgcga
     1741 tacctaaagc cgggaggggc ggcgcctgcg gccctgcaac gtcgaggcgc cgctcctccc
     1801 tggccgtctc aggtgtggca ggccgcccgg gaacgttccc gccaacaggc aatgcgggcc
     1861 cggatctcct cggaggagag tttcaggagc ccgcagacat tttcgaacgc gaacacgtgg
     1921 gtgcgcgcgt ggctggcgat ccagcgctcc gcgtcgcgcc tcttgacgcg cgcctggaag
     1981 ttgcgcgtgg cgtgaccgag ttcgatgcag cgcaccgcgt cctcgaggac ggcgatggcg
     2041 agtctcacct cgccgatgcg ccgcaggcgg ttgtccagcc tctcgaagac atcccggacc
     2101 aacgatcctt ggcgaggacg atccaggatc aggcttccgt ccaatgactg ggaggaacga
     2161 atcatggagt gttctccctc ctgttgaaga atgatgccgt ggattcttgc agggggagcg
     2221 gggagcggct atccgtagga gccggtattc acggctcggg ccgatccgga aagtcgacgg
     2281 cggccgccgg gtggcggcgc ggtgctcgtc agaccgtgat gatgtggttc cggatggcgt
     2341 agcggacgag gtcgctgagc gagccgcact ccagcttgtg catgatgttg gagcggtgcg
     2401 tctcggcggt gcgcacgctg atgccgagga tgtccgcgac ctccttgttg ctcttcccct
     2461 cgccgagcag ctgcacgacc tcccgctcgc ggtgcgtcag cgacaccggc tccgccggcg
     2521 gcgtggtctc cgggttgcgt cccaggctgc gcttcaggac cacctgcgcg acgctcgagg
     2581 tgaagaagat ccgctgccgc cccaccgcct cgacggcgtt gacgaggtcg cggcccgcgt
     2641 ccgacttgag gacgtagcct cgcgcgccgg ccgccaggac ctcgcgcacc acctgctcgg
     2701 actcgtgcat ggtgaggatg aggatgccgc tcttcggcgc ggccttgagg atctgccgcg
     2761 cggcgtcgag cccgttgagc cccggcatcc cgatgtcgag gatgatcacg tccggctgca
     2821 ggcgcttcgc cttctccacc gcctcgcggc cgtccaccgc ctccgcgacc acctgccagc
     2881 cgggtcgcgc ctcgagcagg gcacgcaccc cgtggcgcac cacttcatga tcgtcggcca
     2941 ccagaattcg catcgtcatc aagcctccgc gcgtggcacg tcaatcgcga cgtgcaccgt
     3001 cgatccttgc gtcgtcgatc ggatcgtcat ctgccccccg agctggcgca cccgttcgcg
     3061 catgcccagg agcccgagtc cgagtgtttc gattcccccg cggtcgtcgc cgatcgcgat
     3121 gggcatgccg cgtccctgat cccgcacctc cagctccacg ccgtcctccg tgcgcgtcag
     3181 ctggacgcgg gccgtcgggc tcccggagtg ccgctgcacg ttcgacaggc actcctggac
     3241 gatccggtag agggcgttct cgacttccat cggcaggcgc ccgaagcgcg ccgggaggtc
     3301 cacctcgacg gcgatgccgc tcctctggct gaacccctcg gcgcaccagc gcagcgccgc
     3361 cggcagcccc atctcctcga ggagcggcgg gtgaaggagg tacgacatcg agcggatctg
     3421 gcgcacgcac aactccgcga gggcggcccc ctcgtccgcc agcgcgcggg tccgccggtc
     3481 gaagcgcgag acgccgcgct gcagcacggt cagcttcatc gagagggccg cgagcacctg
     3541 cgccgtgctg tcgtgcaagt cgcgcgccag acgctggcgt tcctcgtcct ggaccttcag
     3601 cagccgcgtc gagagctggc gcagggcctg ctcggtctgg cggcgcacgc cgatctcgtc
     3661 ctcgagggcg cgattggcgt ccgagagctg ccgggtccgg cgctcgatgc gcgcctcgag
     3721 gtcggcgtgc gcggcgcgca gatccgactc ggcccgacgg cgctccatga actgccccat
     3781 ctggctgccg agcgccgtca ggagctgctg atgcgacgcg tccggctcgc gcgttttccg
     3841 gctgaagaag gtcaggaccc cgtgcacctc ctgcccgagg aggatcggcg cccccatgcc
     3901 ggacttcagc cccagctcgg cggcggtctc cgcgcgcacc aggtcatagg aggcctgcag
     3961 gctgcggatc cagatgctcc tcccctcgct ccagatccgg ccggccaggc cgacgccggg
     4021 atggaaggtc atcgactcgg ccgaccgggc gcacagcatc agtcgatccc cgggcgcgtg
     4081 ccagtgcgcg ccgtgccgca gcacgccggc ctcatgatcg accgtccaga cgtgggcgca
     4141 gtcccattcg aagttctcgc agatcacgcg gaggatctcc gggctcgcga cctccacgct
     4201 cgaggcctcg cccagggcgc gcgccgccac gtagctcacc tggatgcgcc gcagcgcctg
     4261 ccgccgctcg ctcagcccgg ccgagaagac gagcatggtg acggccgtga ccgccatgaa
     4321 cacctgcgcg agcagcaggc tttcgttgag cgaggtgtgc gcgaacggac cgtgccccat
     4381 cgcggacggg acgatggaga gaaccgacag ggcggccacc gcggtcaccg atccgatccg
     4441 gccgaagcgc agcgcccccc agatgagcag cgggaagagg gccgagggac ggagcatctc
     4501 ctggacgaga ggtgacgtgc cgccccagaa cacgtagcgc cccaggaagg cgaaggcggc
     4561 gcagagcgcc gccgcctcgg cggcccgcgg aagcgtcaga cggaaagggg gccggtcggc
     4621 ccaggccagt agaagcggcg cgacgaccag cgcccccatg aagtccccga gccaccacga
     4681 agcccaggcg cgtcccaggc tcacgccggg gatcacgccg ccgaggtaac cgctgacgac
     4741 gccgaaggag gcgctgacca ccggggcgag cgctgctccg agcagtacga agcggagcgt
     4801 ctcgccgatc cggtcgaacg aggcgcgcag cccctgggga ccgcgcatca gggcgatggc
     4861 gaacagcgcc tcgagcgtgt tgccggccgc catgccgcag gcggcgagcg ccggcgcccc
     4921 cgcggcgaaa ttcgccgcca gcgcgccgag ggcgatgccg ggccacaacc gcgttccgaa
     4981 caggccgagg gccgcgatgg cgatcccggt ggggggccac accggactcg cgaacccgcc
     5041 gacggcgtcg agcttcaggc cgagccgggc ggcgaggaag taaaccgcgg ccagcaacac
     5101 gatccccacc cagcggctgg gttcgacttc ggcccggagg ggggggcccg gtggcgccgg
     5161 catgtcgttc cgcgcgtcgt ccatggacct cggaatccgg tgattgacct tcatttgtac
     5221 cttagttacg gggattgcgg caaccaggag acattcctcg aaccatcttg accgatcctc
     5281 gcggtgatgg cacattccgg accaaccgtg ctcccgttcc ggacaatctc gaggcgcgcg
     5341 cccgctgtcg cggcggtggc gctggtgctt tgcgccgcgg gggcgggtgg ggcgcccggg
     5401 ccgccgtccg gcgggccgcg ggtgttgtcg gcgcgcacgg tcgtggcggc cttcctcgtc
     5461 gatcgcttcg aggcgcggct cctcaccgcc gacgtgaagc cgcgcccgta tctcgaaccg
     5521 ccggcgccgc cggggctgcg ggacgcgggt gaagggggcg cggcgcaggt ggagatcgcg
     5581 ctccgcgatg cggcgggaac gcggttcact ctccgcccgg agatccgcgg tctctgtctc
     5641 gagcacccgc cgggggaacc gccgcacatc gagggggaca cgatccgcct gcaccgcgag
     5701 acgtttctcg tcgagctgcc ggacctgccc ggggccggcc aggttgaaat cgcgatcggc
     5761 gagcggggcc gcgatcaccg cgccgtccgc cggctcctcg gcgccgcggc cctcgacgcc
     5821 gccccggcgc ccgggccggg aggccacggc ggaagcgcga cggaagggtt cacccccgcg
     5881 cagccgcccg ccctggcgac cgccggccag gtgcactggc ctgaggagtt cagcgatccc
     5941 gacgtcacct tcacctgggg ggacgtgagc gaggcggcga agcggatcaa catcgtcctc
     6001 gtcccggacg gttacaccta cgccgagaag ggaaccctca acgcgcacgc gcaggcgctg
     6061 atcgattatc tgaggacgac gacgccctac cgcgagcacg atcgcttcat caactacacg
     6121 ctggtgtacg cgtattcgag ccagtccggc accgaccagt gcgactgcgc ctccgtcgtc
     6181 gacaccgcca tgaacaccgc gttcccctcc tcgggggacc cgtgcggcgg cagcgccaac
     6241 cgctgcctct actacggctc cgggtgcgac acgagcagcg ccgtcaacat cgcgctcgcc
     6301 gaggcgcgcg cgccggcgca cgacacgacg gtcgtgctcg tcaacaccac ccgctacggc
     6361 ggctgcggcg ggcagcgcgc ggtctacagc gccggcaact ccgcggccac cgacatcgcg
     6421 gcgcacgagc tggggcacag cctcgccggg ctggcggacg agtacgtctc cttcgccggc
     6481 tgcggcggtg gcgccgggga gatcaacacc tcgaccaacg gggtgaacgg ggcctggccg
     6541 gagtggatcg gcgatctcgg cgcgccgcgc ctcggggcgc agtactggtc gcagtgcctc
     6601 tatcgcccgg cgaacgactg caagatgcgc tcgctgttca cgccgttctg cccggtctgc
     6661 aaccagcact ggagcctgac cgtgttcggg catccgcgcg tcgcgttctc ggcgccgctc
     6721 acggccctcg tccctccgga gcagaccctc tccatgccgg tcggaggatc ccaggccttc
     6781 tcggtgacga cgcggctgca gacgacgccg ggggtcacca accggatcac ctggacggtc
     6841 cagggacccg gcgatcccgc gccggtcacg gtggcgaccg gcgtgacgtc ctacagtcgg
     6901 tcgttcccct cggtcggcgc ctacaccgtc cgctgcgacg tcgag
//
LOCUS       DASRIQ010000277         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2188657, whole genome shotgun sequence.
ACCESSION   DASRIQ010000277 DASRIQ010000000
VERSION     DASRIQ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2188657"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="VFD06_06805"
     CDS             complement(<1..1930)
                     /locus_tag="VFD06_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03283.1"
                     /translation="MRLWSAVAAAALLVLGAEASVRDLPPRAARAPSPAPASAADRAG
                     RLAQARDEVARAEAEFGATSPEVARALADEAYLLWGLEEYGTGLPIAERALAIRSAAA
                     RETAELAASRYQAADFRRATGDLAGALEGYRGAIAVWTRLFGPDHVENGSAWHYIGVL
                     HGIAGDPERARAALERALRIRERGLGPNDALVAHTLQGLAHLAARGGDGAAEPLFARA
                     QRIWEKTLGPRDPAVARSLLSRASLRVAAGDPAGARPLLDRALSIRLQAFGPRHYLVA
                     QARRVRADLLAREGDFAAAAVEAEAALDILRASLGPVHPEVASALADRARLLWSAGRR
                     ADAFDSALAAERMAREQFLRSSRDLDDALALRHAAARTSGLDVLLTALAQNGAAAGPA
                     VGAAADLELSAAAREAIDALIRSRGMVLGPARRARSDAVDAGLEEVGHAVPPGSALVA
                     YARYRRVGGIAGPGEPSYLAVVVRPGSVPPAILALGPAAVVDASVESWRAEATRDPIA
                     RGGPDPEAMTLEAGDRLRRRIWDPLAPAIGAADRVFIVPDGAVDLVPFAALPDGADRF
                     LAETGPLFHYLTAERDLVVTTGRRAVGRGVLSIGGPRFDAMGRATAPAGGAGAIRPLC
                     PEFGALSFEPLPGAAEEAR"
     gene            complement(1932..>2317)
                     /locus_tag="VFD06_06810"
     CDS             complement(1932..>2317)
                     /locus_tag="VFD06_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03284.1"
                     /translation="VLQILRSFAPGSGVAPSSEGFVVFSAPARGGTPRTLIGGGPATV
                     RVALPFGAQGGAYEARLERPDGGVVVASETRVLKDGELIEARLDVPAEEGPYRLTLVP
                     LSGRGEPIVYPLQVVADQRLPLPKP"
BASE COUNT          321 a          900 c          873 g          223 t
ORIGIN      
        1 cccgcgcctc ctccgcggcg ccggggagcg gctcgaacga gagggcgccg aactcggggc
       61 acagcggccg gatcgcgccc gcaccgcccg ccggcgccgt ggcgcgcccc atggcgtcga
      121 agcggggacc gccgatcgac agaacgccgc gcccgaccgc ccggcgtccg gtcgtgacga
      181 cgagatcccg ctcggcggtc aggtagtgga acagcggccc tgtctccgcg aggaaacgat
      241 cggcgccgtc cggaagcgcc gcgaagggaa ccagatcgac ggcgccgtcc ggcacgatga
      301 agacccggtc tgccgcgccg atcgcggggg ccaacggatc ccagatcctc cgtcgcaggc
      361 gatcgcccgc ctcgagcgtc atcgcctcgg gatccggccc gccccgggcg atgggatcgc
      421 gggtcgcctc ggcccgccac gactccaccg acgcgtcgac cacggcggcc ggcccgagcg
      481 cgaggatcgc cggcggcacg gacccgggcc ggacgacgac cgccagatac gacggctcgc
      541 ccggcccggc gatgccgccg acccgccgat agcgggcgta cgcgacgagg gcgctgccgg
      601 gcggcacggc atgcccgacc tcttcgagac cggcatccac cgcgtccgac cgcgcgcggc
      661 gcgcgggccc gagcaccatg ccgcgcgagc ggatgagggc gtcgatcgcc tcgcgcgccg
      721 ccgcggacag ctcgagatcg gcagccgccc cgacggccgg cccggccgcg gcgccgttct
      781 gcgccagcgc agtcaggagg acgtcgagcc cggaggtgcg cgcggccgca tggcgcagcg
      841 ccagggcatc gtcgagatcg cgcgaggacc ggaggaactg ctcgcgcgcc atgcgctcgg
      901 cggcgagcgc ggagtcgaat gcatcggcgc gccggcccgc cgaccagagg aggcgcgccc
      961 gatcggcgag ggccgaggcg acctcggggt gcacggggcc gagcgaggcc ctcaggatgt
     1021 cgagggcggc ctccgcctcg accgccgccg ccgcgaaatc cccctcgcgc gcgagcagat
     1081 cggcccgcac gcggcgggcc tgcgccacga gatagtgccg cgggccgaac gcctggagcc
     1141 ggatcgacaa cgcccgatcc aggagcggac gcgccccggc aggatctccg gcggcgaccc
     1201 gcaggctggc gcgggagagg agacttcgag cgaccgccgg gtcgcgcgga ccgagcgtct
     1261 tctcccagat ccgctgcgcg cgcgcgaaga gcggctcggc ggccccgtcc ccgccgcgcg
     1321 ccgccaggtg cgccagcccc tgcagcgtgt gggcgacgag agcatcgttc gggccgaggc
     1381 cacgctcgcg gatcctcagg gcgcgctcga gagcggcgcg cgccctttcc gggtcgccgg
     1441 cgataccgtg cagaaccccg atgtagtgcc aggcggaccc gttctcgacg tgatccggac
     1501 cgaacagccg ggtccagacg gcgatggcgc cgcgataccc ctcgagcgcg ccggcgaggt
     1561 cgccggtggc gcggcggaaa tcggccgcct ggtaccggct tgccgcgagc tcggcggtct
     1621 cgcgcgccgc cgcgctccgg atggcgagcg cccgctcggc gatcggcaac ccggtgccgt
     1681 actcctcgag cccccagagg agatacgcct cgtcggcgag agcgcgcgcc acttcaggcg
     1741 acgtggcgcc gaactcggcc tcggcgcgtg cgacctcgtc ccgcgcctgc gccagacggc
     1801 cggcgcgatc cgcggcggac gccggcgccg gactcggggc gcgcgcggcg cgcgggggaa
     1861 ggtcgcggac cgacgcttcc gctccgagca ccaggagcgc tgcggcggcg acggcggacc
     1921 agagccgcat gtcagggttt cgggagcgga agcctctggt ccgccacgac ctggagcgga
     1981 tagacgatcg gctcgccgcg gccgctcagc gggacgagcg tcagccgata cggaccttcc
     2041 tcggcgggca cgtcgagccg cgcctcgatc agctcgccgt ccttcaggac gcgggtttcg
     2101 gaggcgacga cgaccccacc gtccggtcgc tcgagccggg cctcgtaggc gccgccttgc
     2161 gcgccgaacg gcagcgccac ccgcaccgtc gccggtccgc cgccgatgag cgtgcgcggg
     2221 gtgccgccgc gcgccggcgc ggagaagacc acgaacccct ccgaagacgg cgccaccccc
     2281 gaccccggcg cgaatgacct caggatctgc agcacgc
//
LOCUS       DASRIQ010000278         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3781253, whole genome shotgun sequence.
ACCESSION   DASRIQ010000278 DASRIQ010000000
VERSION     DASRIQ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781253"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFD06_06815"
     CDS             complement(<1..409)
                     /locus_tag="VFD06_06815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZN03285.1"
                     /translation="MPPETLRLDVVTPERVLVAESVDEVILPGSQGSFGVLPGHAPLL
                     TDLDIGPLVYRRGQERRELAVAGGFVEVLPDRVTVLAEVAEAPEEIDVARAEQAKERA
                     EQRLKTAAPDTDYERAQQAMRKALLRAEVGKSRP"
     gene            complement(399..1814)
                     /gene="atpD"
                     /locus_tag="VFD06_06820"
     CDS             complement(399..1814)
                     /gene="atpD"
                     /locus_tag="VFD06_06820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZN03286.1"
                     /translation="MNVGKVVQIIGPVVDVEFAEGTLPAIYNAVRITSDGFDVPQKID
                     VIAEVEQHLGEGRARCVAMEPTDGMVRGMQAADTGAPITVPVGNEVLGRVINVLGRPV
                     DQMGDVKAKHHSPIHRAAPSFEQQNTKLEMFETGIKVIDLIEPYLKGGKTGLFGGAGV
                     GKTVLIQELINNVALKHGGVSVFAGVGERTREGNDLWLEMKESGVISKTALVYGQMTE
                     PPGARLRVGLTGLTVAEHFRDELGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQ
                     PTLATELGELQERITSTVKGSITSVQAIYVPADDYTDPAPATTFAHLDASTNLSRQIS
                     EMGIYPAVDPLASTSRILDPRIVGDEHYGVARQVQGVLQRYKDLQDIIAILGIDELSE
                     DDKLVVARARKIQRFLSQPFFVAEQFTGLKGKFVPIKDTVRGFKEIVEGKHDDLPEQA
                     FYLVGGIEEVLEKAEKMKNAA"
     gene            complement(1811..2284)
                     /locus_tag="VFD06_06825"
     CDS             complement(1811..2284)
                     /locus_tag="VFD06_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZN03287.1"
                     /translation="MRSRLRPDETVRLVVRRHPIAVAWPVTVLLFCLGGLAAAAMSET
                     RAWIYIAAAASATAALWALVRWLTWRVDLWVVTNSRVIDESGVLTVRMMDSPLETIHN
                     VGCEQTLFGRMFGFGRVVIQTAAEHGETTIEGLAQPEELRDAIIEMKEQRRRSVG"
BASE COUNT          328 a          884 c          746 g          393 t
ORIGIN      
        1 ggggccggct cttccccacc tcggcgcgca ggagcgcctt gcgcatcgcc tgctgggcgc
       61 gctcgtaatc ggtgtcgggg gccgcggtct tcagacgctg ctccgcccgc tccttcgcct
      121 gctcggcccg cgcgacgtcg atctcctcgg gggcctccgc cacctccgcc aggacggtga
      181 cgcggtcggg cagcacctcg acgaagccgc cggccacggc cagctcgcgc cgctcctggc
      241 cgcgccggta caccagcggg ccgatgtcga gatcggtcag cagtggagcg tggcccggca
      301 ggaccccgaa cgagccctgc gatcccggca ggatcacctc gtccaccgac tcggcgacga
      361 ggacccgctc cggcgtgacg acgtcgagtc gcagcgtctc aggcggcatt cttcatcttc
      421 tcggccttct cgaggacctc ttcgatgccg ccgacgaggt agaaggcctg ctccggcagg
      481 tcgtcgtgct tcccttcgac gatctccttg aagccgcgca ccgtgtcctt gatcggcacg
      541 aacttcccct tgaggccggt gaactgctcg gccacgaaga agggctgcga caggaagcgc
      601 tggatcttgc gggcccgggc gacgaccagc ttgtcgtcct ccgacagctc gtcgatcccg
      661 aggatggcga tgatgtcctg caggtccttg tagcgctgca ggaccccctg cacctgccgc
      721 gcgaccccgt aatgctcgtc gccgacgatg cgcgggtcga ggatgcgcga ggtggacgcc
      781 agcggatcga ccgccgggta gatccccatc tcggagatct gccgcgacag gttggtggag
      841 gcgtccaggt gggcgaaggt ggtcgccggc gccgggtcgg tgtagtcgtc ggccggcacg
      901 tagatcgcct gcaccgaggt gatcgacccc ttgaccgtcg aggtgatccg ctcctggagc
      961 tccccgagct cggtcgccag cgtcggctgg tagccgaccg ccgacggcat gcggccgagg
     1021 agcgccgaca cctccgaccc cgcctgcgtg aaccggaaga tgttgtcgat gaagagcagc
     1081 acgtcctgcc cgagctcgtc gcggaagtgc tctgcgactg tcagcccggt cagcccgacg
     1141 cgcaggcgcg cccccggcgg ctcggtcatc tggccgtaca ccagcgcggt cttgctgatg
     1201 acccccgact ccttcatctc gagccacagg tcgttcccct cgcgggtgcg ctcgccgacg
     1261 ccggcgaaca ccgacacgcc gccgtgcttc agggcgacgt tgttgatcag ctcctggatg
     1321 aggaccgtct tgccgacgcc ggcgccgccg aacaggccgg tcttcccccc cttgaggtac
     1381 ggctcgatga gatcgatgac cttgatgccg gtctcgaaca tctccagctt ggtgttctgc
     1441 tgctcgaacg acggcgccgc gcggtggatg ggtgagtggt gcttcgcctt gacgtccccc
     1501 atctggtcga ccgggcgccc gagcacgttg atcacccgcc cgagcacctc gtttccgacc
     1561 gggacggtga tcggcgcgcc ggtgtcggcc gcctgcatgc cgcgcaccat gccgtcggtc
     1621 ggctccatgg cgacgcagcg ggcccgcccc tcgcccaggt gctgctccac ctcggcgatg
     1681 acgtcgatct tctgcgggac gtcgaacccg tcgctggtga tgcgcaccgc gttgtagatc
     1741 gccggcagcg tcccctccgc gaactccacg tccaccaccg gtccgatgat ctgcaccacc
     1801 ttgccgacgt tcatcccacg ctccttcgtc tctgctcttt catctcgatg atggcgtcgc
     1861 gcagctcctc cggctgcgcc agcccctcga tcgtcgtttc cccgtgctcg gccgccgtct
     1921 ggatcaccac ccggccgaac ccgaacatcc tgccgaacag cgtctgctca cagccgacgt
     1981 tgtgaatcgt ctcgagcggg ctgtccatca tccggaccgt cagcaccccc gactcgtcga
     2041 tcacccgcga gttcgtcacc acccacaggt ccacgcgcca cgtcagccac cgcaccagcg
     2101 cccacagggc cgccgtcgcg ctcgccgccg cggcgatgta gatccaggcc cgtgtctccg
     2161 acatcgcggc cgcggcgagc cccccgagac agaacagcag gaccgtcacg ggccaggcca
     2221 cggcgatcgg gtgccggcgc accaccagcc ggaccgtctc gtcgggccgc aggcgcgagc
     2281 gcacctttac ttcagcgcct ccgcccctcc gaccacttcg aggatctcct tggtgatggc
     2341 ggcctggcgg a
//
LOCUS       DASRIQ010000279         6038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2446024, whole genome shotgun sequence.
ACCESSION   DASRIQ010000279 DASRIQ010000000
VERSION     DASRIQ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446024"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..2490)
                     /locus_tag="VFD06_06830"
     CDS             complement(145..2490)
                     /locus_tag="VFD06_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN03288.1"
                     /translation="MRSRLRPLPAISLALLLLASSSGPRALGGGRSIVPGDDPASTPP
                     PSPDGGMESVIIRFHPPYDALVAQILAAGGEVRHTFDIVDAVSARVPRAALPLLGRFA
                     GPGSITKDPAIPQPERVTVDPFERILPVERRDDDRTIVAESVEALPTPADVAALAAAH
                     PESYSVNAGFLGVATLHAIGVAGQGTVVAVIDSGLKPGFPHLEIDGSILGGEDFAHDG
                     IAWSSRLNDGHGTFVAGMISGNVMAQLSNGAFLSAVRRYAPGAIVGTNTMPIIGSAPF
                     SGIFVMRVFSPFIQVSSSAVLEAMERVLDLKDAYRQGNPGGLDIGVVNMSLSSVSLDP
                     GRDLMQAAADKLVERGVVVVASAGNAGPSGLTTGSPGSSYASLDVGAASLAHNERILR
                     DLQWGGGVGFLYRPFDGPQTAVFSSRGPIADGRAAPDVTASGYASMGMGFGATNSIQL
                     SSGTSFSSPSVAGVAALLRQGFPQATARQIRNALVLTARPDVLADGSTTSDQGAGYVD
                     GQAAWNLLLAGGASDAPILPPHALSSVAANLVSNAAIKIVSGSLARRVGPLLPGQRAE
                     IFYQVPPNVQAINVTVTPVHPSPPGPENELFGDDLILSVHSAKTGRHRTFFGDGDYLR
                     QTLVIGSDPVVFPLATLEPGIVRITVTGDWTNASEISADLAVAIVQAPSPVQTRLGRI
                     SEFQTIDIPFDVPPGAKRLGARLSWRQGWENIPLNDIDLTLVDPYDFPYYGAATLNVP
                     EEATLLDPPPGRWTAQIYGFEMPTSEDRFKLRIVIDGVVVH"
     gene            2593..2892
                     /locus_tag="VFD06_06835"
     CDS             2593..2892
                     /locus_tag="VFD06_06835"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HZN03289.1"
                     /translation="MARVRVMYWKHIPYAVRAMDEGGPVSRPLPEPFQEAIDALAMIQ
                     GATAAEDYQAGFRWGPTEETPLAAAAAADAAVAAIVAAWPDDRLKAAGRGEDRAE"
     gene            2889..4019
                     /locus_tag="VFD06_06840"
     CDS             2889..4019
                     /locus_tag="VFD06_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03290.1"
                     /translation="MMPRAGTGGPEPGRPPLPAGDPALLPFLQARDEAQAGLLLDDIV
                     ARHAEPVARAAVARHLRAAGAHPGPPPDAEALSAHVLLILRQRLRDLRRPASAPGDTR
                     LFGELRSFLTAQADDACRGWLRGAFPHRIRLAHRLRYLLARDRRFVLRATPDDEWRCG
                     LSGRDARLFASGEASGPGRRIPEAPHGARPIPARLASDPGALSDRAFDLLTRAGGPVD
                     LEHLVDALEAEIALVEPPEAAGAKAERGAFPGPSDAAFETLEAAAGDDPVALLKRLWD
                     QIVALPPLQRAAFLLNLRDGEGRGAIALFPIAGVADLRRIAAVLDMTPERLAERWRAL
                     PLDDAAIAARLGLTRHQVGLLRRAARERLARRIAAGSPATRA"
     gene            4016..5767
                     /locus_tag="VFD06_06845"
     CDS             4016..5767
                     /locus_tag="VFD06_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03291.1"
                     /translation="MTHLGADLLMGYARRTLDAEALLLADDHLSGCPECRGRLIDEDG
                     LKATVRSLWHDLAAARHAPTGHLPYDQIEARAEGRLRGAAREVAETHLEHCAPCAAQT
                     RDLEGFATAFRDARPRATVAGAVAAAAENDAPFPPATRSAGDPAAPHVPASEPQARGA
                     KNGGPRSTLRLSDEALAEEPALRPSAKGTFVFSSPREEKSRSGIVALAIVGAAILVAV
                     AYGGRAGWQRLVALKGKVQELERTNESLQAQAATRAGNEPTAAGGDPAGPSAGATGAT
                     AGAASAGAANDAASGTEGGLAAAPAAETAATAPTLAARRALTIQDRKGPVGLDAEGDL
                     VGFDGLPVAAGKVLAAALRRGGIEVPEEIRSLAAARPVPADPGVGPEPPAPGGGDIVM
                     TLATAAGTFVRSTRPVLSWGAVEGATAYRVAVFDAQGTAIARGGPMRGTKWTVSGPLR
                     RGALYEWRVVAIRPAETGTATAAAGTGDAAVQAAATDAPVAETPVPAAAPRGPSMERI
                     ALGAARFRVLAGDESAALERGLRSAPGSRLVAGVLSARAGLLDDAERELRALAEENPG
                     SDVARGLLASVEAARLP"
     gene            5794..>6038
                     /locus_tag="VFD06_06850"
     CDS             5794..>6038
                     /locus_tag="VFD06_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZN03292.1"
                     /translation="MRQHLDQFTIRTARGLTDVTAQVRRIVAASGIRRGLCTVTIRHT
                     SASLVIQENADPKVLSDLEAFLTRLVPDGDPLFTHND"
BASE COUNT          785 a         2225 c         2268 g          760 t
ORIGIN      
        1 gagatcgtca gcggcaacca ggacctcggc gcgcgcgccc tcgtcgatct catcttccag
       61 aaggtggagg cgttctcggg ccgcacccgc ccggccgacg accagacggt cgtggtggtg
      121 agactcccgc gcctctgacg aggctcagtg gacgacgacg ccgtcgatca cgatccgcag
      181 cttgaaccgg tcctcgctgg tcggcatctc gaaaccgtag atctgcgccg tccacctccc
      241 gggcggcgga tcgaggagcg tcgcctcctc cggcacgttc agcgtggcgg cgccgtagta
      301 ggggaagtcg tacggatcca cgagcgtcag gtcgatgtcg ttgagcggga tgttctccca
      361 cccctgccgc cacgacagcc gcgcccccag gcgcttcgcc ccggggggga catcgaacgg
      421 gatgtcgatc gtctggaact cggagattcg gccgaggcgc gtctgcaccg gtgacggggc
      481 ctgcacgatc gccacggcga ggtcggccga gatctccgag gcgttcgtcc agtccccggt
      541 gaccgtgatc cgcacgatcc ccggctcgag cgtcgcgagc ggaaagacca cgggatcgga
      601 cccgatgacg agggtctggc gcaggtagtc gccgtcgccg aagaacgtgc ggtgccggcc
      661 ggtcttggcg ctgtgcaccg agaggatgag gtcgtcgccg aacagctcgt tctcggggcc
      721 ggggggagag gggtggaccg gcgtcacggt gacgttgatg gcctgcacgt tcggcgggac
      781 ctggtagaag atctccgccc tctgcccggg caggagcggc ccaacccggc gcgccagcga
      841 cccggagacg atcttgatcg cggcgttgct gacgagattg gcggcgacgc tcgacagcgc
      901 gtgcggcggc aggatcgggg cgtccgacgc tccccccgcc aacagcagat tccaggccgc
      961 ctgcccgtcg acgtacccgg ccccctgatc gctcgtggtc gagccgtcgg ccaggacgtc
     1021 gggccgggcg gtcaggacga gggcgttgcg gatctggcgg gcggtcgcct gcgggaatcc
     1081 ctgcctcagg agcgccgcca cgccggcgac gctcgggctc gagaacgagg tcccggagga
     1141 gagttggatg ctgttggtcg cgccgaaccc catccccatc gaggcgtagc cggaggcggt
     1201 gacgtccgga gcggcgcggc cgtcggcgat cggtccgcgc gaggagaaca ccgccgtctg
     1261 cggcccgtcg aaggggcggt agaggaaccc gacgcccccg ccccactgga gatcccgcag
     1321 gatccgctcg ttgtgagcca ggctcgcggc tccgacgtcg aggctggcgt acgacgatcc
     1381 ggggctgccg gtggtgagac cgctcgggcc ggcgttgccg gccgaggcca ccaccacgac
     1441 gccgcgctcg accagcttgt cggcggccgc ctgcatcagg tcgcgccccg ggtcgaggct
     1501 gaccgaggac aggctcatgt tgaccacgcc gatgtcgagc ccgcccgggt tcccctgccg
     1561 gtacgcgtcc ttcaggtcca gcacccgctc catcgcctcg agcacggcgg acgaggagac
     1621 ctggatgaag gggctgaaga cgcgcatgac gaagatgccg gagaacgggg ccgacccgat
     1681 gatcggcatg gtgttcgtgc cgacgatggc gcccggcgcg tagcggcgca cggcgctcag
     1741 gaacgcgccg ttcgacaact gcgccatgac gttcccggag atcatcccgg cgacgaaggt
     1801 gccgtgcccg tcgttgaggc gcgacgacca cgcgatgccg tcgtgcgcga agtcctctcc
     1861 tccgaggatc gacccgtcga tctcgagatg cgggaagccc ggcttcaggc cgctgtcgat
     1921 caccgcgacc accgttccct gcccggcgac cccgatggcg tgcagcgtcg cgaccccgag
     1981 gaagccggcg ttgaccgagt aggactccgg gtgcgccgcg gccagggcgg cgacgtcggc
     2041 cggagtcgga agcgcttcga cgctctcggc gacgatcgtc ctgtcatcgt cgcggcgctc
     2101 caccggcagg atgcgttcga acggatcgac ggtcacccgc tcgggctgag ggatcgccgg
     2161 gtccttggtg atcgatcccg ggccggcgaa gcggccgagc aacggcagcg ccgcccgcgg
     2221 cacccgcgcc gacaccgcgt cgacgatgtc gaaggtgtgc cgcacctccc cgcccgcggc
     2281 gaggatctgt gcgacgagcg cgtcgtaggg gggatggaat cggatgatca ccgactccat
     2341 cccgccgtcc ggggacgggg gaggggtcga cgcgggatcg tctcccggaa cgatcgatcg
     2401 accgcccccc agggcgcgcg gcccggagga tgaggcgagg aggagcaggg ccagactgat
     2461 ggcggggagg gggcggagcc gcgaacgcat cggtgtacct ccgttcaact gcgtgggacg
     2521 ggtgcgtggc agaccctttc tactgcgatc ggtcgccgcg tgcaatacaa tgagttcgca
     2581 ggaggccggt ctttggcgcg cgttcgtgtc atgtactgga agcacatccc ctacgccgtg
     2641 cgggccatgg atgagggcgg cccggtgtcg cgtccgctcc ccgagccgtt ccaggaggcg
     2701 atcgacgcgc tggcgatgat ccagggggcg accgccgcgg aggactacca ggccggcttc
     2761 cgctggggcc ccaccgagga gacgcccctg gccgccgcgg ccgcggccga tgccgcggtc
     2821 gccgccatcg tcgccgcctg gcccgacgac cgcctgaagg cggccgggcg cggcgaggac
     2881 cgggccgagt gatgccgcgc gccggcaccg gcggtccgga gccgggacgt ccgcctctcc
     2941 ccgccggtga cccggccctc ctgccgttcc tgcaggcgcg cgacgaggcg caggcggggc
     3001 tgctgctcga cgacatcgtg gcccgccatg cggagccggt ggcccgcgcc gcggtggcgc
     3061 gccacctgcg cgccgccggc gcccaccccg gccccccgcc cgacgccgag gcgctgagcg
     3121 cgcacgtgct gctcatcctg aggcagcgcc tgcgcgacct gcgccgcccc gcctccgcgc
     3181 ccggcgacac gcgcctcttc ggggagctga ggtcgttcct cacggcgcag gcggacgacg
     3241 cctgccgcgg ctggctgcgc ggcgccttcc cgcaccgtat ccgcctggca caccggctgc
     3301 gctacctcct ggcgcgcgat cggcgcttcg tcctgcgcgc cacgccggac gacgagtggc
     3361 gctgcggtct gtcggggcgc gacgcgcgcc tcttcgcctc cggcgaggcg tccgggccgg
     3421 gacggcggat cccggaggcg ccgcacggcg cccggccgat cccggcgcgg ctcgcgagcg
     3481 atcccggggc gctgtccgat cgcgccttcg acctgctcac gcgcgccggc ggcccggtcg
     3541 atctcgagca cctcgtggat gcgctggagg cggagatcgc tctcgtcgag ccgccggaag
     3601 cagccggtgc gaaggcggag cggggggctt ttccgggacc ctccgacgcc gcgttcgaga
     3661 cgctggaggc ggcggccggc gacgatcccg tggcgctcct gaagcgcctg tgggaccaga
     3721 tcgtcgcgct gccgccgctg cagcgcgcgg cgttcctcct gaacctgcgc gacggcgagg
     3781 ggcgcggcgc catcgcgctc ttccccatcg ccggcgtggc ggatctgcgc cgcatcgccg
     3841 cggtcctcga catgacgccg gagcggctgg ccgagcgctg gcgagccctg ccgctcgacg
     3901 acgccgccat cgccgcccgc ctcgggctga cgcgccacca ggtcgggctc ctccgccggg
     3961 cggcgcgcga gcgcctggcg cggcgcatcg cggccggctc ccccgcgacc cgcgcatgac
     4021 ccacctcggg gccgatctgc tgatggggta cgcccgtcgc accctcgacg ccgaggcgct
     4081 cctgctcgcg gacgatcacc tctccggctg tccggaatgc cgcggccggc tcatcgacga
     4141 ggacggcctc aaggcgacgg tccgctcgct ctggcacgat ctcgccgccg cccggcacgc
     4201 gccgaccggg cacctgccgt acgaccagat cgaggcgcgc gccgaggggc ggctgcgcgg
     4261 cgccgcccgc gaggtggcgg aaacgcacct cgagcactgc gcgccgtgcg ccgcgcagac
     4321 gcgcgacctg gagggattcg ccaccgcctt ccgcgatgcg cggccccgcg ccacggtcgc
     4381 gggggccgtc gcggcggcgg cggagaacga cgcacccttt cccccggcca cacgatccgc
     4441 gggcgatccg gccgccccgc atgtcccggc ctcggaaccc caggcgcgcg gcgcgaagaa
     4501 cggcggcccg cgctcgacgc tccgactttc ggacgaggcg ctggcggaag agccggcgct
     4561 gcgcccgtcg gcgaagggga cgttcgtctt ctcctccccg cgcgaagaga agagccggtc
     4621 gggaatcgtg gcgctcgcga tcgtcggcgc ggcgatcctg gtcgcggtcg cgtacggcgg
     4681 gcgtgccggg tggcagcggc tcgtggcgct caaggggaag gtgcaggagc tcgagcgcac
     4741 gaacgagtcg ctgcaggcgc aggcggccac gcgcgccggg aacgagccga cggcggccgg
     4801 cggcgatccg gcgggcccga gcgcgggggc gaccggcgcc acggccggag ccgcgtccgc
     4861 cggcgcggcg aacgacgccg cgtccggcac ggaaggcggc ctcgccgccg cgcccgccgc
     4921 ggagaccgcg gccacggccc ccacgttggc ggcgcgccgc gcgctgacga ttcaggatcg
     4981 caagggtccg gtcgggctcg acgccgaagg ggatctcgtc ggcttcgacg gtctgccggt
     5041 ggcggccggg aaggtcctcg ccgcggcgct gcgccgcggg ggcatcgagg tcccggagga
     5101 gatccgcagc ctcgccgccg cgcgtccggt gccggccgac cccggcgtcg gccccgagcc
     5161 tccggcgccg ggaggagggg acatcgtcat gaccctcgca accgcggccg gcacgttcgt
     5221 ccgttccacc cgaccggtgc tgtcgtgggg agcggtggaa ggggcgacgg cgtaccgggt
     5281 cgcggtgttc gacgcccagg ggaccgcgat cgcgcggggc gggccgatgc gcgggacgaa
     5341 atggacggtg tcgggcccgc tgcggcgcgg cgccctgtac gagtggcgcg tcgtcgcgat
     5401 ccgtcccgcc gagacgggga ccgccaccgc ggccgcgggg acgggggacg cggcggtgca
     5461 ggctgcggcc acggacgctc cggtcgcgga gacgccggtt ccggcggccg ccccgcgagg
     5521 tccctcgatg gagcgcatcg ccctgggcgc cgcccgcttc agggtcctcg ccggggatga
     5581 atcggcggcg ctcgagcgcg gactgcgctc cgcccccgga tcgcgcctcg tcgccggcgt
     5641 gctgtcggcg cgggccggcc tgctcgacga cgccgagcgg gagctgcggg cgctcgccga
     5701 ggagaacccc ggatccgacg tggcgcgagg gctcctcgcg agcgtcgagg cggcccgcct
     5761 cccctgacct gaggacggac cgccacttcg ccgatgcgcc agcacctcga tcagttcacc
     5821 atccggacgg cccgcggcct gaccgacgtc accgcgcagg tgcggcgcat cgtcgcagcc
     5881 tccgggatcc gccgcggcct ctgcaccgtc acgatccgcc acacctcggc gagcctggtg
     5941 atccaggaga atgccgaccc caaggtcctg agcgacctcg aggcgttcct gacgcgtctg
     6001 gtcccggacg gcgatcccct cttcacccac aacgacga
//
LOCUS       DASRIQ010000280         4139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3231578, whole genome shotgun sequence.
ACCESSION   DASRIQ010000280 DASRIQ010000000
VERSION     DASRIQ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3231578"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2152
                     /locus_tag="VFD06_06855"
     CDS             <1..2152
                     /locus_tag="VFD06_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03293.1"
                     /translation="LDCDGRLDFLLEDENGNGHLDPGEDKDGDGRMDDGTEDRNHNFH
                     LDDVPRPAGDYPYGARTPRAPDRFYTIDQMTGRTSGPYYEDYDDERQRFTLRQDLTLF
                     VPDFYGSHDLKTGLVVEREQFSRAAGGRPVVAPIERQPTEGFSTVRTLLPAESLVTNE
                     ATNLTTGIYVQDAYKPFPNLSFGLGLRFDREATDSFGYTPFDPRRERRLYDRIGALMG
                     GEIRAAEDLTQGNNDGILSQGILGDPMFQGNALQSQAPLLEALRRAALQRMTRHHLTT
                     TILSYELANLFPEIRQGGEVATDTLAAIGVRAQHQEPFRLTNNNLAPRLAVAWDPFFD
                     GRTKLFATWGRYYDKLFLNTIVGEEGPDTINRYYLLDPDGVTGLGVPNRSMGQAISKA
                     PPSATQIDRGLQTPFSDELTIGFERELAPELSIALIYVARDYRQQLQDTDINHELRYG
                     ANGRPVDQLGQLPPAFNPNSLGDSGARLPDARPDLYINNFFFNQILRVGNSNEARYRG
                     LTVEMVKRMSRRWEMQGSYTYSRAQGAAEDFQSRLGNDPSTIESEYGYLDFDQRHVVK
                     LSGGFFLPRDWQIGMTASWGSGLPYSIVSCFFALDNVGYQQFRTLFGTTVLDPGASKL
                     RFETEARNSRRNSAVLNLGGRARKTFVLGRNTAGVFLEVFNILNTDDLHLITFEEAPA
                     EISQVDSPNGQGPLQLNGTRQFGRRFQIGFQINF"
     gene            2167..>4139
                     /gene="recG"
                     /locus_tag="VFD06_06860"
     CDS             2167..>4139
                     /gene="recG"
                     /locus_tag="VFD06_06860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZN03294.1"
                     /translation="MISLDAPLDTLRGIGPARARDLREAGVATVEDLLLHLPLRYEDR
                     TSFLPIAALAPGVRGTIRGRVRSAALRRTRVRGLTIFEVLVEDGTGAIRVIFFNQPYL
                     RTHLTRGREVILYGEAKGSRPGRRGLALESPQIEIAGDEEGAEGIHMGRIVPIHLRLP
                     GLSPRAIRTLVHRLLRGLPADLPDPLPPGLADTRSLMARGRAFREAHFPGEGSDLAAL
                     QARATPAQRRLIFEEFFFLQLGFALERRASDAAGRGGPGLHVDDALRDRLRAVLPFRL
                     TSAQRRALQEIAADLLSPRPMNRLLQGDVGCGKTMVALLAALLVAENGLQTVFMAPTE
                     ILAEQHHRLFTRLLQGRGVPVGLLTASVTGSARRQVLAGLESGALRLVVGTHALVEDE
                     VRFRALRLLVIDEQHRFGVAQRGRLRDKGDRPDVLVMTATPIPRSLALTVYGDLDVSV
                     IDALPPGRRPVLTVLREAAGRARVYEFVRRKVEAGRQAFVVVPVIEETERGDLQAAAS
                     LRERLQREVFPGRPIGLLHGRLPAEEREAVMADFAQGRLPVLVATTVIEVGIDVPNAA
                     VMVVEQADRFGLSQLHQLRGRVGRGAHESYCVLLAGESATDEARQRLQVLCDTSDGFE
                     VARRDLELRGPGQFLGTRQSGMADLRLGDLMRD"
BASE COUNT          609 a         1459 c         1465 g          606 t
ORIGIN      
        1 cctggactgc gatgggcgcc tcgacttcct gctggaggat gagaacggca acggccacct
       61 cgatccgggt gaggacaagg acggcgacgg tcggatggac gacgggaccg aggatcgcaa
      121 ccacaacttc cacctcgacg acgtcccgcg cccggcgggg gactatcctt acggcgcgcg
      181 cacgccgcgg gcgccggatc gcttctacac gatcgatcag atgaccggac ggacctccgg
      241 accctattac gaggactacg acgacgagcg gcagcgcttc accctgcggc aggacctgac
      301 gctgttcgtc cccgacttct acggcagcca cgatctgaag accggcctcg tggtcgagcg
      361 ggagcagttc tccagggcgg ccggtggacg gccggtcgtg gcccccatcg agcgccagcc
      421 gaccgagggg ttcagcacgg tgcgcacgct cctgccggcc gagtcgctgg tgacgaacga
      481 ggcgaccaac ctcaccaccg gcatctacgt gcaggacgcc tacaagccgt tcccgaacct
      541 gagctttggc ctggggctgc gcttcgatcg cgaagcgacc gactcgttcg gctacacgcc
      601 gttcgatccc cgcagggagc gtcgcctgta cgaccggatc ggcgctctca tggggggaga
      661 gattcgcgcc gccgaggacc tgacgcaggg gaacaacgac ggcatcctga gccaggggat
      721 cctgggggac ccgatgttcc aggggaacgc ccttcagagc caggcgccgc tgctcgaggc
      781 gctgcggcgc gcggccctgc agcgcatgac gcgccaccac ctcacgacga ccatcctgtc
      841 ctacgagctg gcgaacctct tcccggagat ccgccagggc ggggaggtgg cgaccgacac
      901 gctcgcggcg atcggtgtcc gcgcacagca tcaggagccg ttccgcctca ccaacaacaa
      961 cctcgcgccg cggctcgcgg tcgcctggga ccctttcttc gacggccgca ccaagctgtt
     1021 cgcgacctgg ggccggtact acgacaagct cttcctgaac accatcgtcg gggaggaggg
     1081 gccggacacg atcaatcgct actacctgct cgacccggac ggcgtcaccg gcctcggggt
     1141 gccgaaccga tcgatggggc aggcgatctc caaggcgccc ccctccgcga cccagatcga
     1201 ccgcgggctg cagacgccct tcagcgacga gctgacgatc gggttcgagc gggaactcgc
     1261 ccccgagctg tcgatcgcgc tgatttacgt ggcgcgcgac taccggcagc agcttcaaga
     1321 taccgacatc aaccacgagc tgcgttacgg agcgaacggg cggccggtcg accagctggg
     1381 ccagctgccg ccggccttca acccgaacag cctcggagac tccggcgccc gcctgccgga
     1441 cgcccggccc gacctgtaca tcaacaactt cttcttcaac cagatcctcc gggtggggaa
     1501 ctccaacgag gcgcgctacc gcggcctgac cgtcgagatg gtgaagcgga tgtcgcgccg
     1561 ctgggagatg cagggctcgt acacctactc gcgcgcgcag ggggcggcgg aggacttcca
     1621 gtcgcggctg gggaacgatc cctcgacgat cgagtcggag tacggctatc tcgacttcga
     1681 tcagcgccac gtggtgaagc tgagcggcgg gttcttcctc ccgcgcgact ggcagatcgg
     1741 gatgacggcg tcctggggat cggggctgcc gtactcgatc gtcagctgct tcttcgccct
     1801 cgacaacgtc gggtaccagc agttccgcac ccttttcggc acgacggtgc tggacccggg
     1861 agcctcgaag ctccggttcg aaaccgaagc gcgcaacagc cgccgcaaca gcgcggtgct
     1921 gaacctcggc ggccgcgccc gcaagacgtt cgtgctgggg cgcaacaccg ccggggtctt
     1981 cctggaggtg ttcaacatcc tgaacaccga cgacctccat ctcatcacct tcgaagaggc
     2041 cccggcggag atctcccagg tcgattcccc caacgggcag gggcccctcc agctgaatgg
     2101 cacgcgccag ttcggccgcc gcttccagat cggcttccag atcaacttct gaccccggat
     2161 cgactcttga tttccctcga cgcgccgctc gacaccctgc gcgggatcgg tcccgcccgg
     2221 gcccgtgatc tgcgcgaggc gggggtggcg accgtcgaag acctgctgct gcacctgccg
     2281 ctgcgctacg aggatcggac ctccttcctg ccgatcgcgg cgctcgcccc gggggtgcgc
     2341 gggacgatcc gcggccgggt gcggagcgcc gcgctgcgac gcacgcgggt gcgcgggctg
     2401 acgatcttcg aggtgctcgt cgaggacggc accggggcga tccgggtgat cttcttcaac
     2461 cagccgtacc tccgcacgca cctgacgcgc ggccgcgagg tgatcctcta cggcgaggcg
     2521 aaggggtcgc gcccgggccg gcgcggcctg gccctggagt cgccgcagat cgagatcgcc
     2581 ggcgacgagg agggggcgga ggggatccac atgggccgga tcgttccgat ccatctgcgc
     2641 ctcccgggac tgtctccgcg cgccatccgg accctggtgc accggctgct gcgcgggctg
     2701 ccggccgatc tccccgatcc gctgccgccg gggctcgccg acacgcgctc gctcatggcg
     2761 cgcggacggg cgttccggga ggcgcacttc ccgggcgaag ggagcgacct ggccgcgctc
     2821 caggcacgcg ccacgccggc gcagcgccgc ctgatcttcg aggagttctt cttcctgcag
     2881 ctcgggttcg ccctcgagcg tcgcgcctcc gacgccgcgg ggcgaggcgg accggggctg
     2941 cacgtcgatg acgcactccg ggatcgcctg cgcgccgtcc tcccgttccg gctgacctcg
     3001 gcgcaacgcc gcgccctgca ggagatcgcc gccgatctcc tgtcaccgcg cccgatgaac
     3061 cggctccttc agggggatgt cggctgcggc aagacgatgg tggcgctcct ggcggccctg
     3121 ctggtggcgg agaacggcct gcagaccgtc ttcatggcac cgaccgagat cctcgccgag
     3181 cagcaccacc ggctgttcac gcgcctcctc caggggcgcg gagtcccggt cggcctgctc
     3241 accgcttcgg tcacggggtc cgcacgccgg caggtgctcg cggggctcga gagcggggcg
     3301 ctgcggctcg tcgtcgggac gcacgccctc gtcgaggacg aggtgcgctt ccgggcgctg
     3361 cggctgctgg tgatcgacga gcagcaccgc ttcggcgtcg cgcagcgggg gcggctgcgc
     3421 gacaaggggg atcgccccga cgtgctggtg atgaccgcga cgccgatccc gcgatcgctc
     3481 gccctgacgg tgtacggcga tctcgacgtc tcggtcatcg acgctctgcc gcccggacgg
     3541 cggccggtcc tgacggtgct gcgtgaggcg gccgggcgcg cgcgcgtcta cgagttcgtc
     3601 cggcggaagg tcgaggcggg gcgccaggcg ttcgtcgtgg tgccggtgat cgaggagacc
     3661 gagcgcggcg acctgcaggc ggcggcgtcg ctccgggagc gcctgcagcg ggaggtgttc
     3721 cccgggcggc cgatcgggct cctgcatggc cggctgccgg cggaggagcg ggaagcggtg
     3781 atggcggact tcgcccaggg ccgcctgccg gtcctcgtcg cgacgacggt gatcgaggtc
     3841 ggcatcgatg tgccgaacgc cgcggtcatg gtggtggagc aggcggaccg cttcggcctg
     3901 tcgcagctgc accagctgcg cggccgggtg gggcgcggcg cgcacgagtc gtactgcgtc
     3961 ctcctggcgg gggagagcgc gaccgacgag gcgcggcagc ggctccaggt gctgtgcgac
     4021 acctccgacg gtttcgaggt ggcgcggcgc gatctcgagc tgcgcggccc gggacagttc
     4081 ctcgggacga ggcagtcggg gatggccgac ctgcggctcg gggacctgat gcgcgacca
//
LOCUS       DASRIQ010000281         5287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_367378, whole genome shotgun sequence.
ACCESSION   DASRIQ010000281 DASRIQ010000000
VERSION     DASRIQ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367378"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VFD06_06865"
     CDS             <1..1550
                     /locus_tag="VFD06_06865"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN03295.1"
                     /translation="PGFKSAADWSVRTMKKWGLADPRFETWGRFGRGWDVVRFRAELT
                     SPTYAVLHGVPRAWSGGTSGPARGEVTLAPFATENEEEEIRADLPRYRAAVDAYERKW
                     KGKLRGRFVLVDPVRKLDLPLKPAVRRYDADGLADLLAAPDPRPVPPIEWPIDKMPAD
                     EEARRAMSAALPGEITADYNTRRLAILDRLNAFLKSEGVLGVIHSDKRGPGGIVFAER
                     AGTWYLDTPTVAPTVVLESEDYNRLARLADRGAGPRVEIDLETRFHDDHPDGFNVVAE
                     IPGGAKRNEVVMLGAHLDSWHGGTGATDNAAGCAVVLEAARILKALGVRLDRTVRVGL
                     WGGEEQGLLGSRGYVKAHFADPVTMALKPEHARLSGYFNLDNGAGKIRGVYLQGNDAM
                     RPVFEAWLAPFKDLGGEAVSIRNTFGTDHKPFDAVGLPAFQFIQDPLDYQTRTHHSDL
                     DVPDRVPPGDLMQAAVVMASCVYHAANRPALLPRKSLPPPLPPKKPLPEETTTQTPAS
                     SERKAAS"
     gene            complement(1511..3049)
                     /locus_tag="VFD06_06870"
     CDS             complement(1511..3049)
                     /locus_tag="VFD06_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168061264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HZN03296.1"
                     /translation="MQAMRSADGTATEAHPLRAPGAILLVSCYELGRQPLSVANAAAI
                     LTASGFAPQALDLARVPIDAEAVRRARLIAVSVPMHTALRLGVAAAERLRALNPEACL
                     CFYGLYAWLNAESLLAGPADAVIGGECDEPLLSLAQSLAGAAATGMMDAAAGAGRNVP
                     GVRTRTSDEGPWLARPRPLVPERSLLPAIDGYVHIEADGERLVAGQVESSRGCRHLCR
                     HCPIPPVYGGRFFVVPAETVLEDARRQVEAGARHLSFADPDFLNGPGHAMKVARALHA
                     AHPGITFDFTAKIEHLVKHRDRLAELRDLGCLFIISAVESIDDRVLRILDKGHTRSDV
                     EAVLAATRAAGLTLRPTFVPFTPWTGLEGQRALIEFIVALDLVDAVDPIQTTIRLLVP
                     PGSLLLEHEEMKPHLAGLDAARFTWTWRHPDPRVDALQIESAAIVEAAARAGEPAEGT
                     FRKLAAAAGATTVPAATDAAGADSRGSRRKRRPAPRLTEPWFCCAEPMEGQLAALRSD
                     DAGV"
     gene            complement(3065..3355)
                     /locus_tag="VFD06_06875"
     CDS             complement(3065..3355)
                     /locus_tag="VFD06_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HZN03297.1"
                     /translation="MDPLDDKEVKERVQKILDEMINPAIAGHGGWVDLLDVKDNNVYV
                     RLGGGCQGCGMVNVTLKQGIEATIREEVPQIAGVIDQTDHAGGSNPYYQPAK"
     gene            3458..4231
                     /locus_tag="VFD06_06880"
     CDS             3458..4231
                     /locus_tag="VFD06_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HZN03298.1"
                     /translation="MIRRTDDNFVEGRAAMQIDIRYQPSYSLAILRLEQNEAIQAEAG
                     AMVSMTANMTVDTTMKGGVLGALTRTVLGGETLFANTFRAEGGPGEITLAPALPGDVN
                     SVQMRGETLLIQSGSFLAGDPGIQLDLKWGGARAFFGSEGLFLLRATGTGPVLLSSYG
                     AIHKITLDGRAPYLCDTGHVVAFGAGLTYEVRRVGGWKSTLLSGEGFVCEFRGTGDLY
                     MQTRSTQAFLSWLIPRIPGRTGGGGGVGSGVLGNILRGD"
     gene            4240..4869
                     /locus_tag="VFD06_06885"
     CDS             4240..4869
                     /locus_tag="VFD06_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZN03299.1"
                     /translation="MLTLYRMKHSTNCERVEMALAYKKLEACSIWVDPEDRSEVNRVS
                     GQDLVPVLMFGETPVIDSMEIVRWIEERFPDRPRLYPAEPAKRAECLLFIDWFNRVWK
                     RPPNELDGELQKPEKDRDPRRVARLGAAMAGSLDLFEQMLTGREYLLGEFGAADIAAF
                     PFLKYASIPVAPDDDDLFHFILRDFQRPGTNHPNVLAWIARMDRRPRLG"
     gene            4866..>5287
                     /locus_tag="VFD06_06890"
     CDS             4866..>5287
                     /locus_tag="VFD06_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZN03300.1"
                     /translation="MSRDVFFCVDIEAFGPVPALYNMVSIGAVVVSKQDLRWVPEEER
                     FYVELKPMAPGFDPGAMKVHGISREHLEAHGVPSDEAMRRLSAFVEARLRKTGGRAVF
                     VGHNAVFDWSYISWYYERYGLPNPFGYKALDSKSLAMGR"
BASE COUNT          781 a         1829 c         1894 g          783 t
ORIGIN      
        1 gtccggggtt caagtccgcc gccgactggt cggtgcggac gatgaagaag tgggggctcg
       61 ccgacccgcg cttcgagacc tgggggcgct tcggtcgcgg ctgggacgtc gtgcgcttcc
      121 gggccgagct gacgtcgccc acttacgccg tgctgcacgg cgtgccgcgc gcctggagcg
      181 gcggcacctc cggcccggcg cgcggggagg tgaccctcgc gcccttcgcc accgagaacg
      241 aggaggagga gatccgcgcc gacctgccgc gctaccgggc cgcggtggac gcctacgaac
      301 gcaagtggaa ggggaagctc cgcggccggt tcgttctggt cgacccggtc cgcaagctcg
      361 acctgcctct gaagccggcg gtgcgccggt acgacgccga cgggctggcg gacctcctgg
      421 ccgcccccga tccccgtccg gtgccgccga tcgagtggcc gatcgacaag atgccggccg
      481 acgaggaggc ccggcgcgcc atgagcgcgg cgctgccggg ggagatcacc gccgactaca
      541 acacccgacg cctggcgatc ctcgatcggc tgaacgcctt cctgaagagc gaaggggtgc
      601 tgggggtgat ccactccgac aagcgcggcc ccgggggcat cgtcttcgcc gagcgggccg
      661 gcacctggta tctcgacacg cccaccgtgg ccccgaccgt cgtgctggaa tcggaggact
      721 acaaccgcct ggcgcgcctc gccgacagag gggccgggcc gcgcgtcgag atcgacctcg
      781 agacccgctt ccacgacgac catccggatg gcttcaacgt cgtcgccgag atcccggggg
      841 gcgcaaagcg caacgaggtc gtgatgcttg gggcgcacct cgactcgtgg cacggcggaa
      901 ccggcgcgac cgacaacgcc gccggctgcg ccgtcgtgct ggaggcggcc cgcatcctca
      961 aggcactcgg ggtgcggctc gatcggacgg tgcgggtggg gctgtggggc ggcgaggagc
     1021 aggggcttct cggatcgcgc ggctacgtca aggcgcactt cgccgacccg gtgaccatgg
     1081 cgctcaagcc ggagcacgcg cggctctccg gctacttcaa cctcgacaac ggcgcgggga
     1141 agatccgcgg cgtctatctg caggggaacg acgcgatgcg gccggtgttc gaggcgtggc
     1201 tcgcgccgtt caaggatctc gggggagagg cggtcagcat ccgcaacacc ttcggcaccg
     1261 accacaagcc gttcgacgcc gtcgggctgc cggccttcca gttcatccag gacccgctcg
     1321 actaccagac ccggacgcac cactccgacc tggacgtgcc ggatcgggtg ccgccggggg
     1381 acctgatgca ggcggcggtc gtgatggcgt cgtgcgtcta ccacgccgcc aaccgaccgg
     1441 cgctcctgcc gaggaagtcg ctgccgccgc cgctgccgcc gaaaaagccg ctgccggagg
     1501 agacgacgac tcagacgccg gcgtcgtcgg agcgcaaagc cgcgagctga ccttccatcg
     1561 gctcggcgca gcagaaccac ggctcggtca gtcgcggcgc cggccgccgc ttccgtcgcg
     1621 atccccgtga atccgcaccc gccgcatcgg ttgcggcggg caccgtcgtc gcccccgcgg
     1681 cggcggcgag tttccggaag gtcccctccg ccggctcgcc ggcccgggcc gccgcttcga
     1741 cgatggcagc gctctcgatc tgcagcgcat cgacgcgcgg gtccgggtgg cgccaggtcc
     1801 aggtgaagcg cgcggcgtcg agccctgcga ggtgcggctt catctcttcg tgctccagga
     1861 gcagcgaccc gggtggcacg aggagccgga tggtcgtctg gatcggatcc acggcgtcga
     1921 cgaggtcgag cgccacgatg aactcgatga gggcgcgctg cccttcgagg ccggtccagg
     1981 gcgtgaacgg cacgaacgtc ggccgcagcg tcagaccggc cgcgcgcgtc gccgcgagca
     2041 ccgcctcgac gtcgctccgc gtgtgcccct tgtcgaggat ccgcagcacc cggtcgtcga
     2101 tcgactcgac cgccgagatg atgaacaggc acccgaggtc gcgcagctcg gcgagccggt
     2161 cgcgatgctt caccaggtgc tcgatcttcg cggtgaagtc gaaggtgatc ccggggtgcg
     2221 cggcgtgcag cgcccgcgcg accttcatgg cgtgaccggg gccgttgagg aagtcggggt
     2281 cggcgaagga caggtggcgc gccccggcct ccacctgccg ccgggcgtcc tcgaggaccg
     2341 tctcggccgg cacgacgaag aagcgtcccc cgtagaccgg cgggatcggg cagtgccggc
     2401 acaggtgcct gcagccgcgg ctcgactcca cctgcccggc gacgagccgt tccccgtccg
     2461 cctcgatgtg cacgtagccg tcgatcgccg gcaagaggct gcgctcaggg acgagcggcc
     2521 ggggccgggc gagccatggg ccttcgtcgg aggtccgcgt gcgcactccc ggaacgtttc
     2581 gtcccgcacc tgcggcggcg tccatcatgc cggtcgcggc agcgccggcg aggctctgcg
     2641 cgagagagag gagcggctcg tcgcactcgc cgccgatgac ggcgtcggcc ggtcctgcga
     2701 ggaggctctc ggcgttgagc caggcgtaga gcccgtagaa acacaggcag gcctccgggt
     2761 tgagggctcg caaccgctcc gccgccgcga cgcccagccg cagcgccgtg tgcatcggca
     2821 cggagacggc gatgagccgc gcccggcgca ccgcctcggc gtcgatcggg acgcgcgcca
     2881 ggtcgagcgc ctgcggcgcg aagccggacg cggtcagaat cgcggcggcg ttcgcgacgc
     2941 tcagcggctg gcggcccagc tcgtaacagg agacgaggag aatcgcgccc ggtgcgcgca
     3001 gcgggtgcgc ttcggtcgcc gtcccgtcgg cggagcgcat cgcctgcatg tccggggccc
     3061 cgccttactt ggcgggctgg tagtaggggt tgctgccgcc ggcgtggtcg gtctgatcga
     3121 tgacgccggc gatctgcggg acctcttcgc ggatcgtcgc ctcgatcccc tgcttcaagg
     3181 tgacgttcac catgccgcag ccctggcatc cgccccccag gcggacgtag acgttgttgt
     3241 ccttcacgtc cagaagatcc acccagccgc cgtggccggc gatcgccggg ttgatcatct
     3301 cgtccaggat cttctgcacc ctttccttga cttccttgtc gtccagaggg tccacgaagg
     3361 ctcctttccg atgagaacgc ggccccattc tagcccagat gcaacccctc ccaccggaag
     3421 aatccccgac atgcaaccgc ggggaggccg gggcggtatg atccggcgca ccgacgacaa
     3481 cttcgtcgag gggagagccg cgatgcagat cgacatccgc taccagccgt cctactcgct
     3541 cgccatcctg cgcctggagc agaacgaggc gatccaggcg gaggccggag cgatggtcag
     3601 catgaccgcc aacatgacgg tggacaccac catgaagggg ggggtgctcg gcgcgctcac
     3661 gcgcaccgtg ctcggcggcg agacgctgtt cgccaacacc ttccgggcgg agggagggcc
     3721 gggggagatc accctcgcgc cggcgctccc cggcgacgtc aactccgtgc agatgcgtgg
     3781 ggagacgctg ctcatccagt ccgggtcgtt cctcgccggc gatcccggca tccagctcga
     3841 cctcaagtgg ggcggagcgc gcgccttctt cgggtcggag gggctcttcc tgctgcgcgc
     3901 tacgggcacc gggccggtgc tgctctcatc gtacggcgcc atccacaaga tcaccctcga
     3961 cggccgcgcg ccgtacctgt gcgacaccgg gcacgtcgtg gcgttcggcg cggggctgac
     4021 gtacgaggtc cgccgcgtcg gcggctggaa gtccacgctc ctgtcgggag aagggttcgt
     4081 ctgcgagttc cgcggcaccg gcgatctcta catgcagacc cgctcgacgc aggcgttcct
     4141 ctcctggctc atcccgcgca tcccggggcg taccgggggc ggcgggggag tggggtccgg
     4201 agtgctgggc aacatcctgc gcggcgacta gggcacgcga tgctgacgct ctaccggatg
     4261 aagcactcga cgaactgcga gcgggtcgag atggcgctgg cgtacaagaa gctcgaagcg
     4321 tgctcgatct gggtcgatcc ggaggaccgt tctgaggtga accgggtgag cggccaggat
     4381 ctggtgccgg tcctgatgtt tggtgagacg ccggtcatcg actcgatgga gatcgtccgc
     4441 tggatcgagg agcggttccc ggatcggccg cgcctgtacc cggcggagcc ggcgaagcgc
     4501 gccgagtgcc tgctgttcat cgactggttc aaccgggtct ggaagcgtcc gccgaacgag
     4561 ttggacggcg agctgcagaa gccggagaag gaccgcgacc cgaggcgcgt cgcgcgcctc
     4621 ggcgccgcca tggcaggctc tcttgacctg ttcgagcaga tgctgaccgg gcgggaatac
     4681 cttctcggcg aattcggcgc cgccgatatc gccgccttcc cgttcctcaa gtacgcctcg
     4741 atcccggtcg ccccggacga cgacgatctg ttccacttca tcctgcgcga cttccagcgg
     4801 cccggcacga accacccgaa cgtcctcgcc tggatcgccc ggatggaccg aagaccgcgc
     4861 ctcggatgag ccgcgatgtc ttcttctgcg tcgatatcga ggccttcggg ccggtgccgg
     4921 cgctctacaa catggtctcc atcggcgcgg tcgtcgtgtc caagcaggat cttcgctggg
     4981 tgccggagga ggagcgcttc tacgtcgaac tgaagccgat ggcgccgggg ttcgatccgg
     5041 gggcgatgaa ggtgcacggc atcagccgcg agcacctcga ggcgcacggc gtgccgtcgg
     5101 acgaggcgat gcggaggctt tcggcgttcg tcgaggcgcg gttgcggaaa accggcggac
     5161 gggcggtgtt cgtcgggcac aacgccgtgt tcgactggtc gtacatctcc tggtattacg
     5221 agcgctacgg cctcccgaac ccgttcggct acaaggcgct cgacagcaag tcgctggcga
     5281 tggggcg
//
LOCUS       DASRIQ010000282         6940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2189559, whole genome shotgun sequence.
ACCESSION   DASRIQ010000282 DASRIQ010000000
VERSION     DASRIQ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189559"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFD06_06895"
     CDS             complement(<1..261)
                     /locus_tag="VFD06_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN03301.1"
                     /translation="MSGLAVGFVGLGIMGRPMAGHILKAGFPLTVFNRTRERARELEA
                     SGARLAATPAEVARAADVIVVMVTDSPDVEAVVAGSGGLAQGV"
     gene            complement(258..791)
                     /locus_tag="VFD06_06900"
     CDS             complement(258..791)
                     /locus_tag="VFD06_06900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN03302.1"
                     /translation="MSSSRPGGSEAPLVVEAMGAEDWSAVRAIYEAGIASGHATFETK
                     APSWEAWDRDHLARPRLVARSAAGAPVLGWAAVVPVSGRCVYGGVADLSVYVAPEARG
                     RGVGRALLAALVRESEEAGLWTLQAGIFPENAASLALHRSLGFRDVGTREKIGRMAGR
                     WRDVLLLERRSPTVGKE"
     gene            complement(805..2025)
                     /locus_tag="VFD06_06905"
     CDS             complement(805..2025)
                     /locus_tag="VFD06_06905"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN03303.1"
                     /translation="MSRWGGLNPYSSLRGLPREAWILCGAVLVNRMGTMVIPFLILYL
                     TRELGVSPSRAALVMAVYGLGAVMTAPLAGRLADRIGPRRIVTVSLLCAGLMLLPYPW
                     LRSYWAIVGLTFAWSVAGEALRPASLSWISGVVPPAQRRAAFALNRLAVNLGMSIGPA
                     VAGFIVAHSFAAIFILDALTSIAAGGILFFATREASPPHLLSPDAPPQAAPPPSQRAL
                     RDPAFLYFLLGCLPVQIVFFQHASTLPLYLVRDLGLTESIYGMMFTINTILIILLEVP
                     LNGATSEWSHRRTLALGALLTGLGFGGVGLVGSAAGVALMVVVWTFGEMILLPGSAAF
                     AADAAPPERRGEYLGIYSMSFSCAFAVAPWLGTSTMELWSPRALWAATALCGVLSALL
                     LARVRIARSPAPGV"
     gene            complement(2063..3610)
                     /locus_tag="VFD06_06910"
     CDS             complement(2063..3610)
                     /locus_tag="VFD06_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZN03304.1"
                     /translation="MTLAGRPGKDHLLDSAGAGVAFLDYDRDGRLDVYLPNGWRIESG
                     RIVERGRHALYRGLPDGAFRDVTDEAGVGGEGCWGTGVTAADYDGDGWTDLFVSCFGV
                     NLLFRNLGGGRFENVAARVGVEAPGWNTAAALFDADADGDLDLYVAAYIETSAEDVLK
                     ATPTLDWKGLEKVAFGPFGLEGAPDHFFRQEAGRFVDATVEAGFADRGLGFGLAVRAG
                     DYDGDGDTDLYVANDSDPNYLYRNEGGGRFREVGVWSGCALDAAGAAQASMGITAGDL
                     FASGRLDLFVTNFSEDYSTLYRNLGNGAFEDVSEETGVGPATWKALSWGTAFGDFDAD
                     GDLDLVVANGHIYPQIDRHPEIVGTYAQPLTLLENRGGVFVDATTTSGPGFAAPAAHR
                     GLATGDYDNDGDLDILVLRLDATPALLRNDTPGGAWLTVVPEGERGGPPPPGTIVRVK
                     AGGRTWVRDVAAGDSYASTHDPRLHFGLGAAEAVDEVVVTWPDGSTTRRTAVPSRRFL
                     VVQRGGA"
     gene            complement(3874..4443)
                     /locus_tag="VFD06_06915"
     CDS             complement(3874..4443)
                     /locus_tag="VFD06_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03305.1"
                     /translation="MSSSRRWRGGIVAAVGSGLVLLAGAVPVRAAEGAGETPSAMVAS
                     YKTLADAILAVKKTEANLVRSILTAAHERAQAEMGKARAALKGNDTKGAQEAIENVAA
                     AVGQIGSEGDSSVGAIRKRLLEGGHHHNADGEAKGIYDEGFVIVTKASKTQFLDAAKA
                     IGQLAKAPKADALEAEWTKVEAAWAALMK"
     gene            complement(4520..5308)
                     /locus_tag="VFD06_06920"
     CDS             complement(4520..5308)
                     /locus_tag="VFD06_06920"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZN03306.1"
                     /translation="MPSRPDPMPTLFVSHGAPTLPLDPGPTGPFLKDLGRRLGRPAGV
                     VCVSAHWDTQSPRVTGSPAPETVHDFGGFPEALYSLRYPAPGDPALAQRVVELLGASG
                     RPAAVDPKRGLDHGAWVPLLLMFPAADVPVVQLSVQSHLDARHHFEMGRALHPLRREG
                     VLILGSGGATHNLGTFGRAPVESPAAPWANAFDEWLRAAIEANRSDDLLAWRERAPDP
                     LRNHPTPEHFMPLFVPLGAATGPGRRIHSTFTFSTFSMAAFEWS"
     gene            complement(5321..>6940)
                     /locus_tag="VFD06_06925"
     CDS             complement(5321..>6940)
                     /locus_tag="VFD06_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03307.1"
                     /translation="DGLKLIDGGAAANLPIGIAQALGAERVIAVDITSPLGDIENLGS
                     LVQIYNQWTGYVTEGNRREDLKRLKRGDVIIRPELGDISFIGFDRAGEAMTIGEQAAR
                     AIIPELRRFATDDATWKKFQESHRLRDRQEYRIDAVTIDNHSWVDDDIVRAHVDIPTG
                     VPYDEQQVTQQVMRLHGLDYSGTIDVRLEQDGDGTTKDNGPPGGGTLALQVPEKPYGH
                     NSLQFGASFIDDFKGENGYSLSFRHLRIAANRRGGEWENIGQLGDVGLARTEFYQPFD
                     AGMKWFTSVGAEVRRTNWDIWSEGDILAEFRVNRDSAHVDFGRVFGRWGEARVGAFYA
                     HEDGGVRVGDPAFPSYSESDAGYGFAFNVDTLDAVVFPHEGTSVQARWYDSREAWGAD
                     LDRTRATLRMGHASEIGKVTLYPVGQGCINLDEPATIGSACALGGFLALSGLGQNELL
                     GDKSVFGSLIAYYALKKFDLGALTQGVYLGLSLEAGQVYLRDDAVAWDTLLTGGSFFF
                     GARTVLGPVYLGYGYTEGGRDRIYFLIGQRF"
BASE COUNT         1078 a         2548 c         2380 g          934 t
ORIGIN      
        1 caccccttgg gcgagcccgc cggagccggc caccaccgcc tcgacatcgg gggaatcggt
       61 caccatgacg acgatgacgt cggcggcccg ggccacctcg gccggcgtcg cggcaaggcg
      121 cgctccggac gcctccagct cgcgggcccg ttcacgggtg cggttgaaca cggtcagcgg
      181 aaagccggcc ttcaggatgt gccccgccat cggccggccc atgatcccga ggccgacgaa
      241 gccgacggcc aggccgctca ttccttcccc accgtcgggc tgcggcgctc gagcaggagc
      301 acgtcgcgcc agcggccggc catccttccg atcttttccc tcgtcccgac gtcgcggaag
      361 ccgagcgagc gatgcagcgc caggctcgcg gcgttctccg ggaagatgcc ggcctgcagc
      421 gtccagaggc cggcctcctc cgattcccgc accagcgcgg cgaggagcgc ccggccgacc
      481 ccccgcccgc gcgcctccgg agcgacgtac acgctgaggt cggcgacgcc gccgtagacg
      541 cagcgccccg acaccggaac gaccgcggcc cagccgagga ccggcgcgcc ggcggccgat
      601 cgggcgacga gccgcgggcg ggcgaggtga tcgcgatccc acgcctccca ggagggggct
      661 ttcgtctcga aggtggcgtg gccactggcg atgccggctt cgtaaatggc ccgtaccgcg
      721 gaccaatcct ccgcgcccat cgcttcgacc acgagggggg cttcggagcc gcccggccgc
      781 gaactgctca tgcctgcggg cattctacac gccgggcgcg ggggatcggg cgatccggac
      841 ccgcgccagg aggagcgccg agaggacgcc acacagcgcc gtcgcggccc acagcgcacg
      901 cggcgaccac agctccatcg tgctcgtccc gagccagggg gcgaccgcga aggcgcagct
      961 gaaggacatg gaatagatcc cgaggtactc gccgcgccgc tcgggagggg ccgcgtcggc
     1021 cgcgaacgcg gcgctccccg gcagcaggat catctcgccg aaggtccaga cgacgaccat
     1081 cagggcgacc cccgcggcgc tccccaccag ccccacgccc ccgaacccca gcccggtcag
     1141 gagcgcgccg agggcgagcg tgcggcggtg cgaccactcc gacgtggcgc cgttgagcgg
     1201 cacctccagc aggatgatga ggatggtgtt gatggtgaac atcatcccgt agatcgactc
     1261 ggtcaggccg agatcgcgca cgaggtacag cggcagggtc gaggcgtgct ggaagaagac
     1321 gatctgcacc ggcaggcatc ccagcaggaa gtagaggaag gctggatcgc gcaacgccct
     1381 ctggctcgga ggcggcgccg cctgcggcgg cgcatccgga gacagtaaat gcggcggcga
     1441 cgcttcgcgc gtcgcgaaga acaggatgcc gccagcggcg atcgacgtca gcgcgtcgag
     1501 gatgaagatc gccgcgaagg aatgcgccac gatgaacccg gcgaccgccg ggccgatgct
     1561 catgccgagg ttgacggcga gccggttgag ggcgaacgcg gcgcgccgct gcgccggcgg
     1621 cacgacgccg ctgatccacg acaggctcgc cggccgcagc gcctccccgg ccaccgacca
     1681 ggcgaaggtc agtccaacga tcgcccagta agagcgcagc cacggatagg gaaggagcat
     1741 cagcccggcg cacaggagcg acacggtcac gatgcgccgc ggcccgatcc ggtcggcgag
     1801 acgcccggcg agcggcgccg tcatgaccgc cccgagcccg tagaccgcca tcaccagcgc
     1861 cgcgcgcgag ggcgacaccc ccagctcgcg cgtcaggtac agaatgagga acgggatcac
     1921 catcgtcccc atccggttca cgagcaccgc gccgcacagg atccaggcct cgcgcggcag
     1981 gccccgcagc gaggagtacg ggttgagccc cccccagcgg ctcacggttc ctcccgccgt
     2041 gccccgcggc cgcagacgcg gctcatgcgc ccccccgctg caccacgagg aaccggcgcg
     2101 acggcaccgc cgtgcgccgg gtcgtcgatc cgtccggcca ggtcacgacg acctcgtcca
     2161 cggcctctgc ggcaccaaga ccgaagtgca gtctcgggtc gtgcgttgac gcataggaat
     2221 cgccggcggc gacatcacgc acccacgtgc gtccgccggc cttgacccga acgatcgtcc
     2281 cgggaggcgg cggcccgccg cgctctcctt cagggacgac ggtcagccag gccccgccgg
     2341 gagtgtcgtt gcgcagcaag gccggggtcg cgtcgaggcg cagcaccagg atgtcgagat
     2401 cgccgtcgtt gtcgtaatcc cccgtcgcga ggccgcggtg cgccgccggt gccgcgaacc
     2461 cgggtccgga ggtcgtcgtg gcatccacga agacaccgcc gcggttctcc aggagcgtca
     2521 gcggctgggc ataggtcccg acgatctccg gatgccgatc gatctgcgga tagatgtggc
     2581 cgttggccac caccagatcg agatcgccgt cggcgtcgaa atcgccgaag gcggtccccc
     2641 acgagagggc cttccaggtc gcagggccga cgccggtctc ctcgctgacg tcctcgaagg
     2701 cgccgttccc gagattgcga tagagggtcg agtagtcctc gctgaagttg gtgacgaaca
     2761 ggtcgaggcg ccccgacgcg aacaggtctc ccgccgtgat ccccatgctc gcctgcgccg
     2821 cgccggcggc gtcaagcgcg cagccggacc agaccccgac ttcgcggaac cgaccgcccc
     2881 cttcgttccg gtacaggtag ttgggatccg agtcgttggc gacgtaaagg tcggtgtcgc
     2941 cgtcgccgtc gtagtctcct gcccgcaccg cgagcccgaa gccgaggcca cggtcggcga
     3001 agccggcctc cacggtcgcg tccacgaacc gtcccgcctc ctgccggaag aaatgatccg
     3061 gcgccccttc gaggccgaac gggccgaacg ccaccttctc gagccccttc cagtcgagcg
     3121 tcggagtcgc cttgagcacg tcttcggccg acgtctcgat gtaggccgcg acgtagagat
     3181 cgagatcgcc gtcggcgtcc gcgtcgaaga gggcggcggc ggtgttccag ccgggggctt
     3241 cgacgccaac ccgcgccgcg acgttctcga agcgcccacc gccgaggttt cggaacaaca
     3301 ggttcactcc gaagcaggag acgaacaggt cggtccaacc gtcgccgtcg tagtccgccg
     3361 cggtcactcc ggtcccccag caaccctcgc cgccgacacc ggcttcgtcg gtgacgtcgc
     3421 ggaaagcgcc gtctggcaga cctcggtaga gggcgtgccg gccgcgctcg acgatgcggc
     3481 ccgactcgat gcgccacccg ttcggaaggt agacgtccag ccgaccgtcg cggtcgtaat
     3541 cgaggaacgc cacccccgcc cccgccgaat cgagcaggtg atctttcccc ggccgccccg
     3601 ccagcgtcac gcggtcgagc ccggcctcct tcgcgacatc gacgaaccgg aagcgtcccg
     3661 gcgtcgcgac gcccggaccg gacgcggtac cgggcccgga tccagcggcg ggggcctgtg
     3721 acgacggtcc ggcgcccggc acggcaggcg gcgcgccccg ggaggcacac cccggactcg
     3781 ccgcgatcgc gggcaggagg acggccgaaa cgacgagcgc cgccacatcg tggcggcgcc
     3841 cccgtgactt cagcacgacg aaagggaccg cggctatttc atcagcgccg cccaggcggc
     3901 ctcgactttg gtccattccg cttcgagggc gtcggccttc ggcgccttgg cgagctgtcc
     3961 gatcgccttt gccgcgtcga ggaactgcgt cttggaggcc ttggtgacga tgacgaaccc
     4021 ctcgtcgtag atccccttcg cctcgccatc ggcgttgtga tggtgccctc cctcgaggag
     4081 ccgcttgcga atggcgccga ccgacgagtc cccttcggac ccgatctgac cgaccgcggc
     4141 cgccacgttc tcgatcgcct cctgcgcgcc tttggtgtcg ttccccttga gcgcggcgcg
     4201 cgccttcccc atctccgcct gggcccgctc gtgggccgcc gtcagaatgc tgcgcaccag
     4261 gttcgcctcg gtcttcttga ccgcgaggat cgcgtcggcc agcgtcttgt acgacgccac
     4321 catggccgac ggcgtctctc ccgccccttc cgccgcccgc accgggaccg ccccggcgag
     4381 caggaccagc ccggagccca ctgccgccac gatcccgccc cgccaccgcc gacttgagct
     4441 catcgttcct ccttgagaag ggtccctgca gcgccgcgag tctagcgagg agccgagcgg
     4501 ctccgcaatg gccgccgtct catgaccact cgaaggcggc catcgagaag gtggagaacg
     4561 tgaacgtcga gtggatccgg cggccggggc cggtggcggc gccgagcggc acgaagaggg
     4621 gcatgaagtg ctcgggcgtc ggatggttgc gcagcgggtc gggcgcccgc tcgcgccacg
     4681 ccaggaggtc gtcgctgcgg ttcgcctcga tggcggcccg cagccactcg tcgaaggcgt
     4741 tcgcccacgg cgcggcgggg gattcgacgg gcgcgcgtcc gaacgtcccg aggttgtggg
     4801 tcgcgccgcc gcttccgagg atcaggactc cttcgcgccg caggggatgg agcgcgcggc
     4861 ccatctcgaa gtgatggcgg gcgtcgaggt gcgactgcac cgagagctgc accaccggga
     4921 cgtccgccgc cggaaacatc aggagcagcg gcacccacgc gccgtgatcg agaccgcgct
     4981 tcgggtccac cgcggccggg cggccggagg cgcccagcag ctcgacgacg cgctgcgcca
     5041 gcgccggatc cccgggcgcc ggataacgga gggaatagag cgcttcggga aagccgccga
     5101 agtcgtggac cgtctccggc gccggcgacc cggtgacccg cggcgactgc gtgtcccagt
     5161 gcgccgagac gcagaccact cccgccggcc ggccgagccg gcggccgaga tccttcagga
     5221 acgggccggt cgggccggga tcgagcggca gcgtgggggc gccgtgcgag acgaacaggg
     5281 tcggcatcgg atcggggcgg ctgggcatga cgcttcgatg ctagaaccgc tggccgatca
     5341 ggaagtagat ccggtcgcgg cccccttccg tgtagccata tccaagatac acggggccga
     5401 ggacggtgcg cgctccgaag aagaacgagc cgccggtcag gagcgtgtcc caggcgacgg
     5461 catcgtcacg gaggtagacc tggccggcct cgagcgagag cccgaggtac accccctgcg
     5521 tcagggcccc gaggtcgaac ttcttgagcg cgtagtacgc gatgagactg ccgaacaccg
     5581 acttgtcccc caggagctcg ttctgcccca gccccgagag ggccaggaag ccgccgaggg
     5641 cgcacgccga tccgatcgtc gccggctcat ccaggttgat gcaaccctgg cccaccggat
     5701 agagcgtgac cttgccgatc tcggaggcat gccccatgcg cagcgtcgcg cgggtgcgat
     5761 cgagatccgc cccccacgcc tcgcgcgagt cgtaccagcg cgcctgcaca ctcgtcccct
     5821 cgtgcgggaa gacgacggcg tcgagggtgt ccacattgaa ggcgaagccg tagccggcgt
     5881 cgctctcgct gtacgagggg aaggccggat cgccgacccg cactccgccg tcctcatggg
     5941 cgtagaaggc cccgacgcgc gcctcccccc agcggccgaa gacccgcccg aaatcgacat
     6001 gcgccgagtc ccggttcacg cggaactcgg cgaggatgtc cccctccgac cagatgtccc
     6061 agttggtgcg gcggacctcc gcgcccaccg aggtgaacca cttcatgccg gcgtcgaacg
     6121 gctggtagaa ctcggtgcgc gccagcccca cgtcaccgag ctgcccgatg ttctcccact
     6181 ctccgccgcg ccggttcgcc gcgatccgga ggtgacggaa cgacagcgaa tagccgttct
     6241 cccccttgaa atcgtcgatg aacgaggccc cgaactgcag gctgttgtgg ccgtacggct
     6301 tctcggggac ctggagggcg agggttcctc cccccggcgg gccgttgtcc ttcgtcgtgc
     6361 cgtcaccgtc ctgctccagg cgaacgtcga tggtcccgct gtagtcgagg ccgtgcagcc
     6421 gcatcacctg ctgggtgacc tgctgctcgt cgtacggcac gccggtcggg atgtcgacgt
     6481 gcgcccggac gatgtcgtcg tccacccagg agtggttgtc gatggtcacc gcgtcgatgc
     6541 ggtactcctg ccgatcgcgc agccgatggc tctcctggaa cttcttccag gtggcgtcgt
     6601 cggtcgcgaa gcggcgcagc tccgggatga tggcgcgggc cgcctgctcg ccgatcgtca
     6661 tcgcctcccc ggcccggtcg aacccgatga agctgatgtc ccccagctcc ggccggatga
     6721 tcacgtcccc ccgcttcagc cgcttgaggt cctcgcgccg gttcccctcg gtgacgtagc
     6781 cggtccactg gttgtagatc tgcaccagcg acccgaggtt ctcgatgtcg ccgagcggcg
     6841 aggtgatgtc cacggcgatc acccgctccg cccccagcgc ctgggcgatg ccgatcggta
     6901 ggttggcggc ggcgccaccg tcgatcagct tgagaccgtc
//
LOCUS       DASRIQ010000283         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_367598, whole genome shotgun sequence.
ACCESSION   DASRIQ010000283 DASRIQ010000000
VERSION     DASRIQ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367598"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2016
                     /locus_tag="VFD06_06930"
     CDS             <1..2016
                     /locus_tag="VFD06_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HZN03308.1"
                     /translation="VVNALSEALDLEIWRDGNVYEMRFERGRTVGAFRQTGTTKRRGT
                     KVHFKPDPTVFEETVFSFDTLSQRLRELAFLNEGVTIVIQDERAENRRHEFSYKGGIV
                     SFVEHLNKNKNPIHPKPIHIRSERDGIAAEVALQYNDGYNEIVFSFANSINTLEGGTH
                     LSGFRSALTRTINNYASRLSSKEAVSLSGDDVREGLTAVISVKLPNPQFEGQTKTKLG
                     NSDVKGLVEAMLNDKLAAYLEENPGVARKILEKGLDAMRAREAARKAKELTRRKGALD
                     SGSLPGKLADCQETDPAKSELFLVEGDSAGGSAKQGRDRRNQAILPLRGKILNVEKAR
                     FDKMLSSQEIRTIITALGTGIGEGDYDHNRLRYHRVIIMTDADVDGSHIRTLLLTFFY
                     RQMPQVIERGHLYIAQPPLYRVKRGKVEEYLAHDDDLNRFLMEQASQERAVKLQGGGR
                     LEGNELRHVLNRLTRYQMHLDLMKKRGIDPGLVERLIEAGLTEIGQFEKPEPLAAVRE
                     AIEATGFRTGELERDEEHGRYRFEVGSSQRGKAPVVVSWDLVSSIEYKTLHALDAELT
                     ALRGPLAVAENGHETGVGGRLELLRYLMDEGKKGVTIQRYKGLGEMNPGQLWETTMNP
                     ETRRMLQVNVADAVEADEIFTVLMGDVVEPRRKFIEENALNVRNLDI"
     gene            2016..>3236
                     /locus_tag="VFD06_06935"
     CDS             2016..>3236
                     /locus_tag="VFD06_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZN03309.1"
                     /translation="MDETRHDIGALTPITLEDEMRRSYLDYAMSVIVGRALPDVRDGF
                     KPVHRRVLYAMHEAGNTHDKPYKKAARIVGDVMGKYHPHGDTAIYDTLVRLAQDFSMR
                     YPIVDGQGNFGSIDGDAPAAMRYTEVRMARLAHEMLADIERDTVDFGPNYDGQLQEPL
                     VLPSRLPNLLVNGSSGIAVGMATNIPPHNLGEICDALVAQIEDPDITIAGLMEHVKGP
                     DFPTAGYLCGMEGIRQAYMTGRGSLVMRARATVEEGGKRGKDRIVITEIPYQVNKTRL
                     IEQIAGLVNDKRVDGITDIRDESDRDGIRVVLDLRRDAVPRVILNSLFKHTQMQESFG
                     AIFLAIVNNRPAVLNLKQMLQHFIDHRREIIVRRTQFDLAKAEERAHILEGLKIALDH
                     LDEMVALIRQARTPD"
BASE COUNT          623 a         1098 c         1083 g          432 t
ORIGIN      
        1 gtcgtgaacg cgctgtcgga ggcgctcgac ctcgagatct ggcgggacgg gaacgtctat
       61 gagatgcgct tcgagcgcgg ccggaccgtc ggcgcattcc ggcagaccgg cacgaccaag
      121 cggcgcggca ccaaggtcca cttcaagccg gacccgacgg tgttcgagga gacggtcttc
      181 tccttcgaca ccctgtcgca gcgcctgcgc gagctggcct tcctgaacga aggggtgacc
      241 atcgtcatcc aggacgagcg cgccgagaac cgccggcacg agttcagcta caagggtggc
      301 atcgtctcgt tcgtcgagca cctcaacaag aacaagaacc cgatccaccc gaagccgatc
      361 cacatccgca gcgagcggga cggcatcgcg gccgaggtgg cgctgcagta caacgacggc
      421 tacaacgaga tcgtcttctc cttcgccaac agcatcaaca ccctggaagg gggaacgcac
      481 ctctctggct tccgctccgc gctgacccgc accatcaaca actacgcgtc gcgcctgtcg
      541 agcaaggagg cggtgtccct ctccggagac gacgtccggg aggggctgac ggcggtgatc
      601 agcgtcaagc tgccgaaccc ccagttcgag gggcagacca agaccaagct cggcaacagc
      661 gacgtgaagg ggctggtcga ggcgatgctc aacgacaagc tcgccgccta cctcgaggaa
      721 aacccggggg tggcccgcaa gatcctcgag aagggactcg atgcgatgcg ggcgcgcgag
      781 gcggcccgca aggccaaaga gctgacccgc aggaaggggg cgctcgattc cgggtcgctc
      841 ccggggaagc tggccgactg ccaggagacc gacccggcca agtccgagct gttcctcgtc
      901 gagggggatt cggccggcgg atcggcgaag caggggcgcg accggcgcaa ccaggcgatc
      961 ttgccgctgc gcgggaagat cctcaacgtc gagaaggccc gcttcgacaa gatgctgtcg
     1021 agccaggaga tccgcaccat catcaccgcg ctgggcaccg gcatcggcga gggggactac
     1081 gaccacaacc gcctgcgcta ccaccgcgtc atcatcatga ccgacgccga tgtggacggc
     1141 tcgcacattc ggacgctcct cctgaccttc ttctaccgcc agatgcccca ggtgattgag
     1201 cgcggccacc tgtacatcgc ccagcccccc ctgtacaggg tgaagcgggg gaaggtggag
     1261 gagtacctgg cgcacgacga tgatctgaac cgcttcctga tggagcaggc atcgcaggag
     1321 cgggcggtga agttgcaggg gggcgggcga ctggaaggga acgagctgcg gcacgtgctg
     1381 aaccgcctga cccgctacca gatgcacctc gacctcatga agaagcgcgg catcgacccg
     1441 gggctggtcg agcgcctcat cgaggcgggc ctgacggaga tcggccagtt cgagaagccg
     1501 gagccgctcg cggcggtgcg cgaggcgatc gaggcgaccg ggttccggac cggtgagctc
     1561 gagcgggacg aggagcacgg ccggtaccgc ttcgaagtcg gctcgtccca gcgcggcaag
     1621 gcgccggtcg tcgtctcctg ggacctggtc tcgtccatcg agtacaagac cctccatgcc
     1681 ctcgatgccg agctgaccgc cctgcgcggc ccgctggcgg tggcggagaa cggccacgag
     1741 acgggcgtcg gcgggcggct ggagctcctg cgctacctca tggacgaagg gaagaagggg
     1801 gtcaccatcc agcgctacaa ggggctcggc gagatgaacc ccgggcagct gtgggagacc
     1861 accatgaacc cggagacccg gcgcatgctc caggtgaacg tcgccgacgc ggtcgaggcg
     1921 gacgagatct tcaccgtcct gatgggggac gtggtcgagc cccgccgcaa gttcatcgaa
     1981 gagaacgccc tcaacgtccg gaacctcgac atctgatgga cgagacccgc cacgacatcg
     2041 gcgcgctgac gccgatcacg ctcgaagacg agatgcgccg gtcgtatctc gactacgcca
     2101 tgagcgtgat cgtcggccgc gccctgcccg acgtccgcga cggcttcaag ccggtgcacc
     2161 gccgcgtgct gtacgccatg cacgaggcgg ggaacacgca cgacaagccg tacaagaagg
     2221 cggcccgcat cgtcggcgac gtgatgggga agtaccaccc gcacggcgac accgccatct
     2281 acgacacgct cgtgcggctg gcgcaggact tctccatgcg ctacccgatc gtggacgggc
     2341 aggggaactt cggctcgatc gacggcgacg ccccggcggc gatgcgctac acggaggtcc
     2401 gcatggcccg gctggcgcac gagatgctgg ccgacatcga aagggacacc gtcgacttcg
     2461 gccccaacta cgacggccag ctccaggagc cgctggtcct gccgagccgg ctgccgaacc
     2521 tgctggtgaa cggctcctcc ggcatcgcgg tcggcatggc gaccaacatc cccccgcaca
     2581 acctcgggga gatctgcgac gcgctcgtgg cccagatcga ggaccccgac atcaccatcg
     2641 ccggcctgat ggagcacgtg aaggggcccg acttcccgac cgccgggtac ctctgcggca
     2701 tggaggggat ccggcaggcc tacatgaccg ggcgcggcag cctcgtgatg cgcgcccgcg
     2761 ccaccgtcga ggaggggggg aagcgcggca aggaccgcat cgtcatcacc gagatccctt
     2821 accaggtgaa caagacccgc ctcatcgagc agatcgccgg gctcgtgaac gacaagcggg
     2881 tggacgggat caccgacatc cgcgacgagt cggaccggga cggcatccgc gtcgtcctcg
     2941 acctgcgccg cgacgcggtg ccgcgggtga tcctgaactc gctcttcaag cacacccaga
     3001 tgcaggagtc gttcggggcg atcttcctgg cgatcgtcaa caaccgcccg gcggtgctga
     3061 acctcaaaca gatgctgcag cacttcatcg atcaccgccg cgagatcatc gtccggcgca
     3121 cccagttcga cctggcaaag gccgaggagc gggcccacat cctcgagggg ctcaagatcg
     3181 cgctcgacca cctcgacgag atggtcgccc tcatccgcca ggcccgcacc ccggac
//
LOCUS       DASRIQ010000284         4109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2986420, whole genome shotgun sequence.
ACCESSION   DASRIQ010000284 DASRIQ010000000
VERSION     DASRIQ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2986420"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VFD06_06940"
     CDS             <1..1257
                     /locus_tag="VFD06_06940"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03310.1"
                     /translation="VYASLLDEDQPSAPILKRIALLQMELERPADAIETLLAAEEADP
                     ADHRIRLLMAQVRDSAGDADGARADCERFLLVEPENLDGLFERARLLRRQGEGAASRR
                     AFEDLIARAARQPLPEPQGTTMITVAWAQVGVLAASARDWDGAARALAQAVALAHDPQ
                     DDLLRLLARTHLERGDADAAERVAAAGLLRSPESEAFETLLAEALLVRGEEAKASAAL
                     TAILARHGHSIEATLGIADTLMRRKRFAEGRDILVDALRRHPGDDRLLFAHGAVLERL
                     GRFEEAERALGRAVVANPNNAMALNYLGYMLAQRGRRLPDSVLYVERAVALDPGNPAY
                     LDSLGFALFKLARYVQAEEMLRTALRYDSFDPAIRDHLGDLLVATGRPAEAMREWEAA
                     IACGHEEPDRIRAKVSRARRALVSGR"
     gene            1290..2012
                     /locus_tag="VFD06_06945"
     CDS             1290..2012
                     /locus_tag="VFD06_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03311.1"
                     /translation="MVIACATGTGGAPRPGASVPHIEGFKALYDARSVTPAKTHRFRL
                     ALALAPDRMRMEVLPPVGGPRLVVTADGRRLMALDPQKRRAEIWNPEQDGVARLLGAA
                     IAASDLRTLLEGRSPCGHAGEPAQDDRSCPFGGGRYRPGPAGDADTLRSATLLDAAGT
                     PLLYLEYPPPGPAEGEWWRSIVLKRPAGASTIRLTLVSGPTRADLDPALFSTEPPERF
                     ESGEVLGDSGLSGTDVGEAQAP"
     gene            2009..2965
                     /gene="ispE"
                     /locus_tag="VFD06_06950"
     CDS             2009..2965
                     /gene="ispE"
                     /locus_tag="VFD06_06950"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZN03312.1"
                     /translation="MNGISVEACAKVNLFLRILGLRPDGFHELWSVAQSISLADRLDL
                     EPAPGGIQLEVDGDPEVPSGPDNLVHRAAVRMLGHDPHPGVAMTLVKRIPHGAGLGGG
                     SSDAAAALLGIDRMFGLGTPSGTLREHAAALGSDVPFFLEGGTAVLRGRGTEIEALPD
                     GPHLELLILDPRRPLSTALVYAQVQEPLTLAGKTASIPGFGRIPQDPLSRVRLGNDLE
                     THAARLCPDIPEMRRLLLEGGARVAAMTGSGSAVFGLFDRAETAQEAAGRAEQRGFRA
                     LPCRTLHRSEVGAGRFSARAPGVGPSGGAGHGDHRRPGVPCR"
     gene            2928..3254
                     /gene="spoVG"
                     /locus_tag="VFD06_06955"
     CDS             2928..3254
                     /gene="spoVG"
                     /locus_tag="VFD06_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HZN03313.1"
                     /translation="MEITDVRVFPVDEDKLKAFVSIVIDDCFVISDIKIISGQTGLFI
                     SMPSKKRKNGTFRDIAHPLNSETRRRIEERILARYEETLKERGTGEGGGNDAPPDPSG
                     QGPEGA"
     gene            3257..3330
                     /locus_tag="VFD06_06960"
     tRNA            3257..3330
                     /locus_tag="VFD06_06960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3289..3291,aa:Gln,seq:ttg)
     gene            3360..>4109
                     /locus_tag="VFD06_06965"
     CDS             3360..>4109
                     /locus_tag="VFD06_06965"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZN03314.1"
                     /translation="MTAAGVDGDLKVFTGNAHPALAGEICDQLGVSLGAAEVRRFADG
                     EAYCHIQENIRGTDVFVIQPTCPPVDANLLELLVMLDAFKRSSAARVTAVLPYYGYAR
                     QDRKDKPRVPITAKLVADLLTAAGASRILAMDLHAPAIQGFFDIPVDHLFAAPVLIEH
                     VARMELKDLVVVAPDAGGVERARAYAKRLDASLAIIDKRRGEKNEPQVMHVIGEVAKK
                     TCLIVDDMIDTAGTLVGTVQALTKEGATAIYG"
BASE COUNT          607 a         1469 c         1469 g          564 t
ORIGIN      
        1 gtgtacgcct ccctcctcga cgaggaccag ccctctgcgc cgatcctcaa gcggatcgcc
       61 ctcctgcaga tggagctcga gcggccggcc gacgccatcg agacgctcct ggccgcggag
      121 gaggccgacc cggcggacca tcggatccgg ctgctcatgg cgcaggtgcg cgacagcgcc
      181 ggcgacgccg acggcgcgcg cgccgactgc gagcgcttcc tcctggtcga gccggagaat
      241 ctcgacgggc tgttcgagcg ggcccggctg ctgcgccgcc agggcgaggg ggcggcctcg
      301 cgccgcgcct tcgaggacct gattgcgcgc gccgcccggc agccgctgcc cgagccgcag
      361 gggacgacga tgatcaccgt ggcgtgggcg caggtcgggg tgctggccgc gtcggcccgc
      421 gactgggacg gcgccgcccg ggccctcgcg caggcggtgg cgctggcgca cgacccccag
      481 gacgatctcc tccgactgct ggcgcggacc cacctcgagc gcggggatgc ggacgcggcg
      541 gagcgggtgg cggcggccgg tctgctgcgc agccccgagt ccgaagcgtt cgagacgctt
      601 ctggccgaag cgctcctcgt ccgcggcgag gaggcgaagg cctccgcagc cctgaccgcg
      661 atcctcgcgc gccacgggca cagcatcgag gcgaccctgg gcatcgccga caccctgatg
      721 cgccgcaagc gtttcgccga ggggcgggac atcctcgtgg atgcgctgcg ccggcacccg
      781 ggggacgacc gcctcctctt cgcgcacggc gccgtgctcg agcgcctggg acggttcgag
      841 gaggcggagc gcgccctcgg ccgcgccgtg gtcgccaacc cgaacaacgc catggcgctc
      901 aactacctcg gctacatgct ggcgcagcgc ggacgcaggc tgccggactc ggtcctgtac
      961 gtggagcggg cggtggcgct cgatcccggg aacccggcgt acctcgacag tctcggcttc
     1021 gcgctcttca agctggcccg ctatgttcag gccgaggaga tgctgcgcac cgccctgcgc
     1081 tacgattcct tcgatccggc gatccgggat catctcggcg acctgctggt cgccaccggg
     1141 cggccggcgg aggcgatgcg ggaatgggaa gcggcgatcg cgtgcggcca cgaggagccc
     1201 gatcggatcc gcgccaaagt ctcccgcgcg cgccgcgccc tcgtctccgg ccgctaacaa
     1261 ctttcctcgc gctcctcatc ggatcggcga tggtcatcgc ctgcgccacc ggaaccggcg
     1321 gcgcgccccg tcccggagcg tcggtcccgc acatcgaggg gttcaaggcg ctctacgatg
     1381 cccgcagcgt caccccggcg aagacccacc gcttccggct ggcgctcgcg ctggcgccgg
     1441 atcggatgcg gatggaggtc ctgcctccgg tgggagggcc ccgactcgtc gtgaccgcgg
     1501 atgggcggcg tctcatggcg ctcgatccgc agaagcgccg cgccgagatc tggaacccgg
     1561 agcaggatgg agtggcgcgg ctcctcggag cggcgatcgc cgcctccgac ctgcgaaccc
     1621 tgctcgaggg ccggtccccg tgcggccacg cgggtgaacc cgcgcaggac gatcgcagct
     1681 gcccgttcgg gggcggcagg tatcggcccg gaccggccgg cgatgccgac acgcttcgga
     1741 gcgccacgct cctcgacgcc gccggaactc cgctgctcta cctcgaatac ccgccgccgg
     1801 ggccagccga aggggagtgg tggaggtcga tcgtgctcaa gcgaccggcg ggagcttcca
     1861 cgatccggct caccctcgtg tccggcccga cgcgcgccga cctcgacccg gcgctgttct
     1921 cgaccgagcc gcccgaaaga ttcgagtccg gcgaggtcct gggggattcc gggttgtccg
     1981 ggacggacgt cggggaggcc caggcgccat gaacgggatc tcggtcgaag cgtgcgccaa
     2041 ggtcaatctc ttcctcagga tcctggggtt gcgtcccgac ggcttccacg agctgtggag
     2101 cgtcgcgcag agcatctccc tggccgaccg actcgacctg gagcccgccc cgggcggaat
     2161 ccagctcgag gtggacggag atccggaggt cccgtccgga ccggacaacc tcgtccaccg
     2221 ggcggcggtc cgcatgctgg gtcacgaccc gcatccgggc gtggcgatga ccctggtcaa
     2281 gaggatcccg cacggggccg ggctcggcgg cggcagcagc gacgccgccg ccgccctgct
     2341 cggcatcgac cggatgttcg gcctcggcac cccgtcgggg acgctgcggg agcacgccgc
     2401 agccctcgga tcggatgtcc cgttctttct cgaggggggg accgccgtgc tccggggccg
     2461 cgggaccgaa atcgaggcgc tgccggacgg tccccacctt gagctgctga tcctcgaccc
     2521 gcggcggccc ctttcgaccg ccctcgttta cgcgcaggtt caagagccgt tgacacttgc
     2581 gggcaaaacc gctagcattc ccggcttcgg gcgcatcccg caggatccgt tgtcccgggt
     2641 gcggctcggc aacgatctcg aaacgcacgc ggcgcggctc tgtccggaca tcccggagat
     2701 gcggcgcctg ctcctcgagg gcggtgcccg ggtcgcggcc atgaccggga gcggctcggc
     2761 cgtcttcggc ctgttcgaca gggcggaaac ggcgcaggag gcggccggaa gggcggagca
     2821 gcggggcttc agggccctgc cgtgccgcac gcttcatcgg agcgaggtcg gagcgggccg
     2881 attctccgcc agggcgcccg gagtcgggcc gtcaggtggg gccggacatg gagatcaccg
     2941 acgtccgggt gttccctgtc gatgaggaca aactcaaggc gttcgtctcc atcgtcatcg
     3001 acgactgctt cgtgatcagc gacatcaaga tcatcagcgg acagaccggg ctgttcatct
     3061 cgatgccgag caagaagcgg aagaacggca ccttccgcga catcgcgcat ccgctcaaca
     3121 gcgagacgcg ccggcgcatc gaggagcgca tcctcgcgcg ctacgaggag acgctgaagg
     3181 aacggggaac gggcgagggc ggcggaaacg acgcgccgcc cgatccttcg ggacagggtc
     3241 ccgagggcgc gtgaggtggg gcgtcgtcaa gcggtaagac actggacttt ggatccagca
     3301 ccggaggttc gaatcctccc gccccagcca tcggccggga gcgggcgagg cgaggcggca
     3361 tgacggcggc gggggtagac ggcgacctca aggttttcac cggcaacgcg catccggcgc
     3421 tcgccggcga gatctgcgat cagctcggcg tgtcgctcgg ggcggccgag gtgcgccgct
     3481 tcgcggacgg cgaggcgtac tgccatatcc aggagaacat ccgcggcacc gacgtcttcg
     3541 tcatccagcc cacctgcccg ccggtcgacg ccaacctgct cgaactgctc gtcatgctgg
     3601 acgccttcaa gcgatcctcg gcggcgcgcg tcaccgcggt gctgccgtac tacggctacg
     3661 cccgccagga tcgcaaggac aagccgcgcg tcccgatcac cgccaagctg gtcgcggatc
     3721 tcctgacggc cgcaggggcc agccgcatcc tggcgatgga tctgcacgcc cccgccatcc
     3781 agggattctt cgacatcccg gtggaccacc tgttcgccgc gccggtgctc atcgagcacg
     3841 tggcccgcat ggagctcaag gacctcgtcg tggtggcccc ggacgccggc ggcgtcgagc
     3901 gggcccgcgc ctacgccaag cggctcgacg cctcgctcgc aatcatcgac aagcgccgcg
     3961 gcgagaagaa cgagccccag gtgatgcacg tcatcggcga agtcgcgaag aagacctgcc
     4021 tcatcgtcga cgacatgatc gacacggcgg ggaccctcgt cggcacggtg caggcgctca
     4081 cgaaggaagg ggcgacggcg atctacggc
//
LOCUS       DASRIQ010000285         6170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1937566, whole genome shotgun sequence.
ACCESSION   DASRIQ010000285 DASRIQ010000000
VERSION     DASRIQ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937566"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1614)
                     /locus_tag="VFD06_06970"
     CDS             complement(<1..1614)
                     /locus_tag="VFD06_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN03315.1"
                     /translation="MALSRGTKLGAYEVIGPIGAGGMGEVYRARDTRLQRDVAIKVLP
                     DLQNAEPERLARMAREAQALAALNHPNIAQIHGLEESGGVRALVMELVEGEDLSTLIA
                     RGPMPFAEARPIARQIAEALESAHEQGIVHRDLKPANVKVRQDGTVKVLDFGLAKITD
                     PLSSASGGRGDLALSPTLTAQATRAGVILGTAAYMSPEQARGRMVDRRADVWAFGVVL
                     YEMLTGRRAFEGEDISITLANVLKEDVDWRALPADLPPPTRRLLRRCLDKDPRRRLSA
                     MGDARLELDDSLARDADAGAATTAAARGRRGVTIAIGLAIAAGAALLTAGVMRAWAPT
                     AAPAPAPGVTRLAIALPEGAEVTETNRLPLALSPDGTMLAYIGRHEGGVRQLYLKRLG
                     DRDPIALQDTDGAISPFFSPDGQWIAFFAQGKIKRIAVGGAALQIVASEVPDPRGGTW
                     TDDGQIYFTPTNTSGLYKVPAAGGAATPVTKLDPEKDEISHRWPEALPGGRSLLFTVW
                     TGPGSDERRIVVQSLESGERHALVPGGDSA"
     gene            complement(1677..5612)
                     /locus_tag="VFD06_06975"
     CDS             complement(1677..5612)
                     /locus_tag="VFD06_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZN03316.1"
                     /translation="MRLIAVAGCVALTAISAQGAGVATRALTFEQRIQAQEAIERVTY
                     AHQIGATRPFEEVVTRDVLERKVRTSLRQSAALEAFWKTPVTSEMLRREMERIVTGTR
                     MPARLRELFEALGNDPVLIQECLARPALVDRLTRSFFASDAVVHEKARREAEALHEEL
                     ESGRLDPRERHPRRTLFELVRASGVEAVPEPGGSAADDARNVIRLDPEAFEKHTARLP
                     QRIGAIGAMEEDSDSFVTRVVLDSSATRIEFASYLVAKVPWDEWWASVEATLEWESLA
                     TVALSDVLVPNLPSRSRVPADDSCALDDEWEDGLRDVPDPRSDHTAVWTGNVMIVWGG
                     YDGRFLDTGSRYDPVIDVWARTSRTGAPSGRRLHTAVWTGTSMVVWGGAGAGTFNTGG
                     RYDPATDVWTPTTLTNAPAARDSHVAVWTGSRMIVWAGTQPSGGRYDPAADSWSGLST
                     AGAPNPARSATAIWTGNLMIVWGGLVGFNPIGYASGGRYNPATDTWSPVSASGPYSRV
                     GHTAVWTGTRMIVWGGRQDSTRLDSGGQYNPVTDSWTLTSLSGAPTPRSQHTAIWTGS
                     LMIVWGGLEGGPQGTAASTGGRYDPAADVWTPVSTGNVPERRSGHSAVWTGGQMLVWG
                     GTSLSRLNSGGRYDPATDSWTPTAVSFSPPGRRDFTAVWTGSHLIVWGGAASASLNSG
                     GRYDPTLDVWSPTSTIDAPSARVKHTAVWTGNSMVVWGGSSGPRFATGGRYDPVADVW
                     VPTSTIDAPSARERHTAVWTGDVMIVWGGYDGSSLDTGSRYDPVSDVWSPMSLLGDHP
                     AARADHTAVWTGSLMIVWGGNNPSLGHVNSGGRYDPVGDVWSQTSTINAPSPRLRHTA
                     TWAGDVMVVWGGSGSTAGYLGTGGRYDPLSDAWTPTSTANAPLSRFSHSAIWTGDLVV
                     VWGGQGQGQGFLNTGGRYNPINDLWTATSTVEAPSLRSEHRAVWTGTSMVVWGGFDGA
                     YPETWGRYVVAPFVDEDGDGFDSCKDDCDDADAAIHPGAAELCDARDNDCSGTADGFP
                     TSCGDPLCVQTGMCIAGVDNCVPLTPGPEACNSIDDDCDGSVDEDFDVDHDGFTTCEG
                     DCDDGRAAVHPGVAEVCDALDNDCNGVVDGFTTWCGTGGCFASGVCSEGANTCVPGLP
                     SAEYCDLVDNDCDGPIDEIPVGSVTVMVESDQDASLLTWDWLEDATAFDVVLGDVGIL
                     RATGGDFTAAIQGCLVDEWGDTTLTLGATPDPGAAFFYLVRGGSCLGQGTYDSGASSQ
                     VGLRDAEIDTSPSSCPAVPPFCGDGYCSPEESCDCFDCYWCCYFGGCGP"
     gene            5857..>6170
                     /locus_tag="VFD06_06980"
     CDS             5857..>6170
                     /locus_tag="VFD06_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03317.1"
                     /translation="MKPPAEGGSGPLADAPRRRDAPAEMSPEEFREAGHVLVNRIADW
                     LGRMPKGPVTRAATPRALRTVIGEGPLPSEGTPALPALEEAARLLFDHSLFNGHPRFW
                     GY"
BASE COUNT          966 a         2155 c         2103 g          946 t
ORIGIN      
        1 cgcgctgtcg ccgcccggaa ccagtgcgtg ccgctcgccg ctctcgagag actgcaccac
       61 gatccggcgc tcgtccgacc ccggcccggt ccagacggtg aagaggagcg accggccgcc
      121 gggaagggcc tcaggccagc gatggctgat ctcgtccttt tcgggatcga gcttcgtcac
      181 cggagtcgcg gcgccccccg cggcgggaac cttgtacaac ccggaggtgt tggtcggagt
      241 gaaatagatc tggccgtcgt ccgtccaggt cccaccgcgc gggtccggga cctcgctcgc
      301 cacgatctgc agcgccgcac caccgacggc gatcctcttg atctttccct gtgcgaagaa
      361 ggcgatccac tggccgtcgg gcgagaagaa gggggaaatg gccccgtccg tgtcctgcag
      421 ggcgatcggg tcccgatccc ccagccgctt caagtagagc tgccgaacgc ccccctcgtg
      481 ccggccgata taggcgagca tcgtgccgtc cggtgagaga gcgaggggca gccggttcgt
      541 ctcggtgacc tccgccccct ccggcagcgc gatggcgagg cgggtcacgc cgggcgccgg
      601 agcaggcgcc gccgtgggcg cccaggcacg catcacgccg gcggtcagaa gggcggcgcc
      661 ggccgcgatc gccaggccga tcgcgatcgt gacgccgcgg cgcccgcgcg cggcggccgt
      721 ggtcgcggcg cccgcgtcgg cgtcgcgcgc gaggctgtcg tcgagctcca gtcgcgcgtc
      781 ccccatcgcg gagagccgcc gccgcggatc cttgtcgagg cagcggcgca gcagccggcg
      841 cgtggggggc ggcagatcgg ccggcaacgc ccgccagtcc acatcctctt tcaggacgtt
      901 ggccagcgtg atcgagatgt cctccccctc gaaggcgcgc cggccggtca gcatctcgta
      961 gagcacgacg ccgaaggccc agacgtcggc gcgccggtcg accatgcggc cgcgcgcctg
     1021 ctccggcgac atgtaggccg ccgttccgag gatcactccg gcgcgcgtcg cctgcgccgt
     1081 gagcgtcgga gacagcgcga ggtcgccgcg gccgccggac gcgctgctca gcgggtcggt
     1141 gatcttcgcc agtccgaagt ccagcacctt gacggtgccg tcctggcgca ccttgacgtt
     1201 cgccggcttc agatcgcggt ggacgattcc ctgctcgtgc gccgactcga gcgcctcggc
     1261 gatctgccgc gcgatcggcc gcgcctcggc gaacggcatc ggcccccgcg cgatgagcgt
     1321 cgagaggtcc tccccttcga ccagctccat caccagggcg cgcacgcccc ccgactcctc
     1381 gagcccatgg atctgcgcga tgttcggatg gttgagcgcg gcgagcgcct gcgcctcgcg
     1441 cgccatccgc gcaagccgct ccggctcggc gttctgcagg tcgggcagaa ccttgatggc
     1501 cacgtcgcgt tgcaggcggg tgtcgcgggc gcgatagacc tcccccatcc cgccggctcc
     1561 gatcgggccg atcacctcgt acgctcccag tttcgtgccg cggctcaaag ccatgaggaa
     1621 cggagcgtag cagagaacgc ttcccggtca ccggatcgat gtccccccaa ggaccctcat
     1681 gggccgcacc ccccgaagta gcagcaccaa tagcagtcga agcagtcgca tgattcctcc
     1741 ggactgcagt aaccgtcccc gcaaaaggga ggcactgctg gacaggagga aggcgatgtg
     1801 tcgatctccg cgtcgcgcag ccccacttgt gaggacgcgc ctgagtcgta tgttccctgg
     1861 cccaggcagc tccccccgcg caccagatag aagaacgccg cgcccggatc gggcgtcgcg
     1921 ccgagcgtca gtgtcgtgtc tccccattca tcgacgagac agccctggat ggccgctgtg
     1981 aaatcgccgc cggtcgctcg caggatcccg acgtcgccga ggacgacgtc gaacgccgta
     2041 gcgtcctcca gccagtccca ggtgagcagg gacgcgtcct ggtcggactc gaccatgacg
     2101 gtcacgcttc caacggggat ttcatcgatc ggtccatcgc aatcgttgtc gacgaggtcg
     2161 caatactcgg cggacggaag acctggaacg caggtgttcg ctccctccga gcagaccccg
     2221 gaggcgaagc accccccggt gccacaccag gtggtgaagc cgtcgacgac gccgttgcag
     2281 tcattgtcga gggcatcgca gacctccgcc acgcctgggt ggaccgccgc cctcccgtcg
     2341 tcgcagtctc cctcgcaggt cgtgaatccg tcgtgatcga cgtcgaagtc ctcgtcgacc
     2401 gagccgtcgc agtcgtcgtc gatgctgttg caggcttcgg gccccggcgt gagcgggaca
     2461 cagttgtcca cgcccgcgat gcacatcccg gtctggacgc aaagcgggtc cccgcaggac
     2521 gtggggaaac cgtcggctgt cccgctgcaa tcgttgtccc gggcatcgca cagctctgcc
     2581 gctcccggat gaatggcggc atccgcgtcg tcacaatcgt ccttgcaact gtcgaacccg
     2641 tccccgtctt catcgacgaa aggggccacg acgtagcgac cccaggtctc cgggtacgcc
     2701 ccgtcaaaac ctccccaaac caccatcgag gttcccgtcc agacggcgcg gtgctcggat
     2761 cggagggaag gagcctcgac ggtcgaggtc gcggtccaga gatcgttgat cgggttgtag
     2821 cgcccgccgg tgttgaggaa tccctggccc tggccttgac caccccagac gacgaccaga
     2881 tcgccggtcc agatcgcgga atgactgaaa cgggacaatg gtgcattggc cgtcgacgtg
     2941 ggtgtccagg catcggaaag cggatcgtat cggccgccgg tgccgagata gcctgcggtg
     3001 gaaccggatc ctccccagac gaccatgaca tctccggccc aggtcgcggt gtgtcgcagg
     3061 cggggggacg gcgcattgat cgtggaggtc tgcgaccaga catccccgac cgggtcgtat
     3121 cgacctccgc tgttgacatg gccgaggctg gggttgttgc cgccccagac gatcatgagg
     3181 ctcccggtcc agacggcggt gtggtctgcc cgcgcggccg gatggtcgcc gagcaaggac
     3241 atcggggacc agacgtcgga gacgggatcg tagcggctgc ccgtgtcgag agagctgccg
     3301 tcgtagccgc cccacacgat catgacgtct cccgtccaga cggcagtgtg cctctcccgt
     3361 gcggacggtg catcgatcgt cgaggtcggg acccagacgt cggcgacagg gtcgtacctg
     3421 ccgccggtgg caaaacgggg cccactggac cctccccaca cgaccatcga gtttccggtc
     3481 cacaccgcgg tgtgtttcac acgggcggaa ggagcatcga tggtcgaagt cggactccag
     3541 acgtcgaggg tcggatcgta ccggccgccg ctgttcagcg acgcggaggc ggcgccgccc
     3601 caaacgatga gatggctccc cgtccagacc gcggtgaagt cgcgccggcc tggcggtgag
     3661 aaactgaccg ccgtgggtgt ccacgagtcg gtcgccgggt cgtagcgacc accgctgttg
     3721 agacgggaca gggaggttcc gccccagacg agcatctggc ccccagtcca gacggccgag
     3781 tgccccgacc ggcgctcggg gacattcccc gtggagacgg gagtccacac gtcggcggcg
     3841 ggatcgtacc ggcccccggt gcttgccgcg gtcccctggg gacctccttc caaaccgccc
     3901 cacacaatca tgaggctgcc ggtccagatc gcggtgtgct gggagcgcgg cgtcggggcg
     3961 ccggaaaggg aagtcagcgt ccaggagtcg gtgacgggat tatattgccc gcctgagtcc
     4021 aaccttgtcg agtcctgtcg cccgccccag acgatcatgc gcgttcccgt ccagaccgcg
     4081 gtgtgcccca cgcgcgaata cggcccgctg gcggacacag gactccaggt atccgtggcg
     4141 ggattgtaac gtcccccgga ggcatacccg atcgggttga agccgacgag tccaccccag
     4201 acgatcatga ggtttcccgt ccagatcgcg gtggcggaac gcgccgggtt gggcgctcca
     4261 gcggtggaca acccggacca gctatcggcg gccgggtcgt accgaccgcc cgacggttgc
     4321 gtgccggccc agacgatcat gcggcttccg gtccacacgg cgacgtggga gtcccgggcc
     4381 gcgggcgcgt tggtcagcgt ggtcggagtc cagacatcgg tcgccgggtc gtaccgtcca
     4441 cctgtgttga acgtgcccgc gccggccccc ccccagacca ccatggaagt ccccgtccag
     4501 acggcggtgt gcaagcgacg acccgatggt gcgccggtcc tggaggtgcg cgcccagaca
     4561 tcaatcaccg ggtcgtaacg gctgccggta tcgaggaacc ggccgtcgta gccgccccag
     4621 acgatcatga cgttcccggt ccagacggcg gtgtggtcgc tgcgcggatc ggggacgtcc
     4681 cggagtccgt cttcccactc gtcgtcgagg gcacacgaat cgtccgcggg aacgcgggag
     4741 cgggaaggaa ggttcggaac gaggacatcg gacagggcga cggtcgccag cgactcccat
     4801 tccagggtcg cttcgaccga ggcccaccat tcgtcccatg gcaccttcgc cacgaggtag
     4861 ctcgcaaact cgatccgcgt ggcggaactg tcgaggacga cgcgcgtgac gaaggagtcg
     4921 gagtcctcct ccatcgcgcc gatcgcgccg atgcgttggg gaaggcgggc ggtgtgcttc
     4981 tcgaacgctt cggggtcgag tcggatcacg ttcctcgcgt cgtcagcagc gctgccgccc
     5041 ggttccggaa ctgcttcgac gccgctcgcc cgcaccagct cgaagagggt ccggcggggg
     5101 tgccgctcgc gcggatcgag acgaccgctt tcgagttcct cgtggagggc ttccgcttcc
     5161 cggcgcgcct tctcgtggac cacggcgtcg gaggcgaaga agctccgggt caaccggtcc
     5221 acgagggcgg gccgcgccag gcactcctgg atcaggaccg ggtcgtttcc cagggcttcg
     5281 aagagctccc gcaacctcgc gggcatgcgc gtgcccgtga cgatccgctc catctccctg
     5341 cggagcatct cggacgtcac gggcgtcttc cagaacgcct cgagggcggc cgattgcctg
     5401 agtgaggtcc ggacctttct ctccagcacg tcccgggtga cgacctcctc gaacggccgc
     5461 gtggcgccga tctggtgggc gtaggtgacg cgctcgatcg cctcctgcgc ctggatgcgc
     5521 tgttcgaacg tgagcgcgcg ggttgcaacg ccggctccct gggccgaaat ggccgtcagg
     5581 gccacacagc ccgcaaccgc gatcagtcgc atgggtccct ccgatcagac gtcggcgacg
     5641 tcagggaatg aaggccagct cgggatgggg cagcctcggc aagcgcgaca ggcgaccgtg
     5701 atgctccccc ctcgaaatcc catgcgctca aggtcgtttc gggggcgtct tggttcggga
     5761 atgaatgggg ggccactcgt gcggccgcgt cgtaccggct tccacccgga tccgtcgctc
     5821 ctgacgtata tagggcgcac aggaggttcc gtggagatga aaccaccggc cgaagggggc
     5881 tccggccctc tcgccgacgc gccgcggcgc cgggacgcac cggccgagat gagcccggag
     5941 gagttccgcg aagccgggca cgtgctcgtc aatcggatcg ccgactggct cggcaggatg
     6001 ccaaaggggc cggtgacccg ggccgcgaca ccgcgcgccc tgcgcaccgt catcggcgag
     6061 gggccgctgc catccgaggg gacgcccgcg ctcccggcgc tcgaggaggc ggcacgcctt
     6121 ctcttcgatc actcgctgtt caacgggcac ccgcgcttct ggggctacat
//
LOCUS       DASRIQ010000286         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_892605, whole genome shotgun sequence.
ACCESSION   DASRIQ010000286 DASRIQ010000000
VERSION     DASRIQ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892605"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1568
                     /locus_tag="VFD06_06985"
     CDS             174..1568
                     /locus_tag="VFD06_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZN03318.1"
                     /translation="MPKSRGKSKQRKPARQRAVPVPSSAQRGLGRAAKVVRAAAARAA
                     RASTSLFGGEGSRRVVLLVGTRKGAWILVGDAERRRFRVEGPHFLGCIVNHVVLDPRD
                     RRTLLASAKTGHLGPTVYHSTDLGRTWAEAVRPPAFPKVAVAAGATAGDSPSETAAVE
                     PASAANGPDGGGASKGRAVDANFWLTPGHISQPEVWWAGTVPHGLFRSHDGGVTWDGI
                     DGFNRNPAGVSWDAPGFFSTPDGAITHSVLIDPRDADHMYVGLSIGGLFETRDGGVHW
                     TPLNRGVAADFLPDPTVPYGHDPHCVALHPKNPDRLYQANHCGTYRLDRPGDTWERIG
                     RNMPKEIGDIGFPIVLHPRDPDTAWVFPMDGTTVWPRTSPGGRPAVYRTRDAGATWER
                     QDGGLPKGQGWFTVKRQAMAADAGDPVGLYFGTTSGEIWGSADEGRTWRPILAHLPQI
                     LSIAAAEVPAAALP"
     gene            1565..1849
                     /locus_tag="VFD06_06990"
     CDS             1565..1849
                     /locus_tag="VFD06_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZN03319.1"
                     /translation="MTGEAAGPHRVHIPSPLRSYTAGRAEVEASGGTLAAILADLEAA
                     FAGIRFRMIDEQDRIRPHILIFVAGRPARDLRARVEPGDEVQIVAALSGG"
     gene            1853..>2629
                     /locus_tag="VFD06_06995"
     CDS             1853..>2629
                     /locus_tag="VFD06_06995"
                     /inference="COORDINATES: protein motif:HMM:NF016087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HZN03320.1"
                     /translation="MTTRSALAFVRKRGMVLMAARGPVPSLAEAIAGAPLRGSWWGHP
                     RGRLMFRIFEALADSKEILVCRLVDGKITFVHRRIWPALVRLAPRLPKRGLAAVRQEH
                     TESGRHRNVITPFPDWVPKDVRRAGERLDVAAAERLLGAAAGALSGSARRRTPPRAGR
                     PGRQGTARRLATRLAVIALLVTGIAPLAGCGGSGKGPAVAAPAASEVPGTPVDAPEVP
                     YATTYAGDIPCADCPGQRLTLTLFPDGTWRMRRVYRGAEAG"
BASE COUNT          334 a          988 c          971 g          336 t
ORIGIN      
        1 gagtcggacg acttcggcgg ccccggcgcc gatttcggcg gagacgattt cggcggggac
       61 gcggggggtg gcggcgacat gggcggcgag cacggcggag acatggacgc ctcgtccggc
      121 ggatgcggca actgcggcga cccccgcggg cccggctcct gcgccgtcaa ctgatgccga
      181 agtcccgcgg aaaatcgaaa caacggaaac cggcgcgcca gcgggcggtg ccggtcccct
      241 cgtccgcgca gcgcggcctc gggcgcgcgg cgaaggtggt ccgggccgcc gcggcgcgcg
      301 ccgcccgcgc ctccacgagc ctcttcggcg gcgagggatc gcgtcgggtc gtcctcctcg
      361 tcggcacgcg caagggggcc tggatcctcg tcggcgacgc cgagaggcgc cgcttccgtg
      421 tcgaggggcc gcacttcctc ggctgcatcg tcaatcacgt cgtcctcgat ccgcgcgatc
      481 gacgcacgct cctcgcctcg gcgaagaccg ggcacctggg gccgaccgtc tatcactcca
      541 ccgatctcgg acgcacctgg gccgaggcgg tccgcccgcc cgcgttcccc aaggtcgcgg
      601 ttgcggcggg cgcgaccgcg ggagattcgc cctccgaaac agcggcggtc gaaccggctt
      661 ccgccgcgaa cgggccggac ggcggcggcg cgtcgaaggg gcgcgcggtg gacgcgaatt
      721 tctggctgac cccgggccac atcagccagc cggaggtgtg gtgggccgga acggtcccgc
      781 acgggctctt ccgctcgcat gacggcggcg tcacctggga cgggatcgac ggcttcaacc
      841 gcaatccggc cggcgtgtcg tgggacgcgc cggggttctt cagcacgccg gacggcgcca
      901 tcacccactc ggtcctcatc gatccgcgcg acgccgatca catgtacgtc ggcctgtcga
      961 tcggcggcct gttcgagacg cgcgacggcg gcgtgcactg gacgccgctc aaccgcggcg
     1021 tcgccgccga cttcctcccc gacccgaccg tgccgtacgg gcacgacccg cactgcgtgg
     1081 cgctccatcc gaagaacccc gatcgcctct atcaggccaa ccactgcggc acctaccgcc
     1141 tcgatcgccc gggcgacacc tgggagcgga tcggccgcaa catgccgaag gagatcggcg
     1201 acatcggctt cccgatcgtc ctgcacccgc gcgatccgga caccgcctgg gtcttcccga
     1261 tggacggcac caccgtctgg ccgcgcacca gccccggcgg ccggccggcc gtctaccgga
     1321 cgcgcgacgc cggcgccacc tgggagcggc aggacggcgg gctgccgaag gggcaggggt
     1381 ggttcacggt caagcgccag gcgatggccg ccgatgcggg ggatccggtc gggctctact
     1441 tcgggaccac cagcggcgag atctggggga gcgccgacga ggggcgcacc tggcggccga
     1501 tcctcgcgca cctgccgcag atcctctcga tcgccgccgc cgaggtcccg gccgcggcgc
     1561 tgccgtgacc ggcgaggccg ccggcccgca ccgcgtccac atcccctccc cgctccgctc
     1621 ctacacggcc gggcgcgccg aggtcgaagc ttcgggcggc accctcgccg cgatcctcgc
     1681 cgatctcgag gccgccttcg ccggcatccg cttccggatg atcgacgagc aggatcgcat
     1741 ccggccgcat atcctgatct tcgtcgccgg ccggccggcg cgcgatctcc gggcgcgggt
     1801 cgagccgggc gacgaggtgc agatcgtggc ggctctgagc ggaggctgaa aaatgaccac
     1861 tcgttcggcg ctcgcattcg tccggaaacg cggcatggtg ctgatggcgg cgcgcgggcc
     1921 ggtcccgagc ctggcggagg cgatcgccgg cgccccgttg cgcggctcgt ggtgggggca
     1981 cccgcgcgga cggctcatgt tccggatctt cgaggcgctc gccgactcga aggagatcct
     2041 cgtctgcagg ctggtggacg ggaagatcac cttcgtccac cggcggatct ggccggctct
     2101 cgtgcgcctg gcgcctcgcc tcccgaagcg cgggctcgcg gccgtccggc aggagcacac
     2161 ggaaagcggc cggcaccgga acgtcatcac gccgtttccc gactgggtgc cgaaggatgt
     2221 gcgtcgcgcg ggagagcggc tcgacgtcgc ggcggcggag cggcttctcg gcgccgccgc
     2281 gggcgcgctg tccggcagcg cgcgccggcg cacgcctccc cgcgccggcc ggccgggccg
     2341 ccagggcacg gcgcggcgtc tcgcgacgcg cctggccgtc atcgcgcttc tcgtcaccgg
     2401 catcgcgccg ctcgcggggt gcggcggatc cggaaagggg cccgcggtcg ccgcgcccgc
     2461 cgcgtcggag gtgccgggga ccccggtgga cgcgccggag gtgccgtacg cgacgacgta
     2521 cgcgggagac atcccctgcg ccgactgccc cggccagcgg ctgaccctca ccctcttccc
     2581 ggacggaacg tggcggatgc gccgcgtgta ccgcggcgcg gaggcgggg
//
LOCUS       DASRIQ010000287         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3785439, whole genome shotgun sequence.
ACCESSION   DASRIQ010000287 DASRIQ010000000
VERSION     DASRIQ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3785439"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /gene="carB"
                     /locus_tag="VFD06_07000"
     CDS             complement(<1..1447)
                     /gene="carB"
                     /locus_tag="VFD06_07000"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZN03321.1"
                     /translation="MPKRTDIHSILIIGSGPIVIGQGCEFDYSGVQACRALREEGYRI
                     VLINSNPATIMTDPEFADATYVEPITPEAVEKILRREREHGTPVDAVLPTLGGQTGLN
                     TAMACYDKGIFTKYGVKMIGANREAIFKGEDRQAFKDLMIGIGLKVPASRVVHTLEEA
                     RAAMDEVVGLPAIIRPAFTLGGTGGGIAYNVEEFEAIVQRGLDASPVTEVLIEQSVIG
                     WKEYELEVMRDRNDNVVIICSIENLDPMGVHTGDSITVAPVQTLTDKEYQRMRDAAIA
                     VIRAVGVETGGSNIQFGVNPDNGEMVVIEMNPRVSRSSALASKATGYPIAKIAAKLAL
                     GYTLDELRNDITRDTPASFEPTIDYVVVKIPRFNFEKFPRADRTLTTSMRSVGEVMAI
                     GRTFSEAYLKALRSMEASRLALEPVDLPADKEEREKVLTEALRVPRPERPWYVAQAFR
                     EGFSVEQVHELSAIDPWFLRKIEDLVHRAQAL"
     gene            1950..>2753
                     /locus_tag="VFD06_07005"
     CDS             1950..>2753
                     /locus_tag="VFD06_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03322.1"
                     /translation="MRLSSLFRRKNRQQPGVAPRPFEQLEGRLLFAVGDPDDDVLPPV
                     IEFFAPVADGLIGNDGTASRFYRYSAFDPSGVSNTNWQLTRDTGSGPEVILSALAAPR
                     EGTIDIAPFGLGTFRLTVTATDNDTDDGDLSDRSTASASRSITIVDDDTAGPLIVFSQ
                     PNVPVGNDGTLPRRFLFTVNDASLLSTAGYSLTRDTGSGPVEIATGAVNLDSNTVDIA
                     PYGTGAFTLTVTATDGDNDRGGDASTSTLSHSITITDDDTAGPLIVFSEP"
BASE COUNT          417 a          942 c          916 g          478 t
ORIGIN      
        1 gcagcgcctg ggcccggtgc accagatcct cgatcttccg caggaaccac gggtcgatgg
       61 cggacagttc gtgcacctgc tccacggaga agccctcgcg gaaggcctgg gccacgtacc
      121 aggggcgctc ggggcggggg acgcggagcg cctcggtgag caccttctcg cgctcctcct
      181 tgtcggcagg cagatccacc ggctccagcg ccagccggct ggcctccatg gagcgcaggg
      241 ccttcaggta cgcctctgag aaggtgcggc caatggccat cacctcgccc acggagcgca
      301 tgctcgtggt gagcgtccgg tccgctcggg ggaacttctc gaagttgaag cgcgggatct
      361 tcaccaccac gtagtcgatg gtgggctcga aggaggccgg cgtgtcgcga gtaatgtcgt
      421 tgcgcagctc atccagcgtg tagcccagcg ccagcttcgc ggcgatcttg gcgatggggt
      481 agcccgtcgc cttgctcgcc agggcggagc tgcgcgagac gcgcgggttc atctcgatga
      541 cgaccatctc cccgttgtcg gggttgacgc cgaactggat gttcgacccg cccgtctcga
      601 cgccgacggc gcggatgacc gcgatcgccg cgtcgcgcat ccgctggtac tccttgtcgg
      661 tcagcgtctg caccggcgcg acggtgatcg agtcgccggt gtggacgccc atcgggtcga
      721 ggttctcgat cgagcagatg atgacgacgt tgtcgttgcg gtcgcgcatc acctccagct
      781 cgtactcctt ccacccgatc acgctctgct cgatgagcac ctcggtgacc gggctggcgt
      841 cgaggccgcg ctggacgatc gcctcgaact cctcgacgtt gtaggcgatg cccccgcccg
      901 tccccccgag cgtgaacgcc gggcggatga tcgccggcag ccccacgacc tcgtccatcg
      961 ccgcccgcgc ctcctccagc gtgtgcacca cgcggctggc cggcaccttg aggccgatgc
     1021 cgatcatcag gtccttgaag gcctggcggt cctcgccctt gaagatcgcc tcgcggttgg
     1081 ccccgatcat cttcacgccg tatttcgtga agatcccctt gtcgtaacag gccatcgcgg
     1141 tgttgaggcc ggtctggccg ccgagggtgg gcaggacggc gtcgacgggg gtgccgtgtt
     1201 cgcgctcgcg cctgaggatc ttttcgaccg cctccggggt gatcggctcg acgtaagtgg
     1261 cgtcggcgaa ctccgggtcg gtcatgatcg tagcgggatt ggagttgatg aggacgatcc
     1321 ggtacccctc ctcccgcagc gcccggcagg cctgtacgcc ggaatagtcg aactcgcacc
     1381 cctggccgat gacgatcggg ccggagccga tgatcaggat cgagtggatg tcggtgcgct
     1441 tgggcatcgt ggcaagtcgt tctcgggacg tctgaggccg gttgccgcgc ccccgccccg
     1501 gccggtgcaa aattcggggc aatgtaacgg cggcgcagcg cgttgggaag tgtcagccgt
     1561 cgggcgtcgc gtagcggata tgcggcgtcc gggggcggtg gaatcgcccg acagcaatgt
     1621 atttgaaaag tgccggttga cgtgatatga gtgtaagttc aggggatgag gcggcagaag
     1681 accggccgcc tcccggggtt ttccgttaag agcggtaatc taataagacg tcaggcctgg
     1741 cgctaggggt ttttcccggg cggggcctgc atcccgcggc cagaagggga tcggaaggtc
     1801 cggccgccgg gagtcgtgtt ttcttcaaga acaatcctgg tttgtcttcg ccgtgctgcc
     1861 ggcgcgcgcc cgcgcgcaca gccccgagca tttgggcgcg gcacggacgc ttcacgccgg
     1921 caatgagccg gaatcgtcag gagactttca tgcgcctgtc gtccctgttt cgccggaaga
     1981 atcgccagca gcccggcgtt gcgccgcggc cgttcgagca gttggagggc cggctgctgt
     2041 tcgccgtcgg cgaccccgac gacgacgtcc tgccgccggt gatcgagttc ttcgccccgg
     2101 tcgccgacgg cctcatcggc aatgacggca ccgccagccg cttctaccgc tacagcgcgt
     2161 tcgacccgtc cggcgtgtcc aacacgaact ggcagctcac ccgtgacacc gggtcgggcc
     2221 ccgaggtgat cctgtccgca ttggcggcgc cccgtgaggg cactatcgac atcgccccct
     2281 tcggcctggg caccttccgc ctgaccgtca ccgccaccga caacgacacc gacgacggcg
     2341 acctctccga ccgctcgacc gcgtcggcga gccgttccat cacgatcgtc gacgacgaca
     2401 ccgccggtcc gctgatcgtc ttctcgcagc ccaacgtgcc cgtcggcaac gacggcaccc
     2461 tgccgcggcg gttcctgttc accgtcaatg acgcttcgct gttgtcgacc gcgggctact
     2521 cgctgacgcg cgacaccggc agcgggccgg tcgagatcgc gacgggcgcc gtgaacctgg
     2581 acagcaacac cgtagacatc gcgccgtacg gcaccggcgc gttcaccctc accgtgacag
     2641 ccaccgacgg ggacaacgac cgcggcggcg acgcgtcgac gtcgacattg agccactcca
     2701 ttaccatcac ggacgacgac acggccgggc cgctcatcgt cttctccgag ccg
//
LOCUS       DASRIQ010000288         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_893167, whole genome shotgun sequence.
ACCESSION   DASRIQ010000288 DASRIQ010000000
VERSION     DASRIQ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893167"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VFD06_07010"
     CDS             complement(<1..1529)
                     /locus_tag="VFD06_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZN03323.1"
                     /translation="MSRTAWRASAFLAMLGPAALLSLAPFSAAPAAAAPQARLVRIGL
                     STDSVRSRITADGGVIVLDPVKRTPIWRKVFDPGLTFVAEVQGKGPLVLYRVQIGSFA
                     TEDGANQKKAAIESLLPAEKVVVAYHPDRRSWRVRAGEFRTREEAATLVKRLADEGYN
                     ELWIVEDEPETAGRRRVRLVDERWHDFLAATDRVLLQPARSGAWLKVDATPYRGTLEV
                     RVDRAGRIRVINVLELEEYLRGVVPNEMGPGVYPELEALKAQAVAARTYIVSNLGQFA
                     DSGYDVCDSAQCQVYKGAGTEHPLTNQAVEETRGLVAVQDGRPINALYTSTCGGHTED
                     GALIFPEEKGSYLKGVECYPEAEAEGHALTGVEWVEPVTLEDGARVNEEIQMMRLLGI
                     VGNEALDRSWLQDRCKVSEAERWTTYTLGQIGKKPDHEGLRDNRLQLHDYVVYLTRSL
                     GWDEKMRLALDARDLPYLLAFRDRDLVPEEARRAYAELILEGIFDPFPDNTLRPTHEP
                     SR"
     gene            complement(1553..2167)
                     /gene="folE"
                     /locus_tag="VFD06_07015"
     CDS             complement(1553..2167)
                     /gene="folE"
                     /locus_tag="VFD06_07015"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HZN03324.1"
                     /translation="MKGRRSGKAGRVPPSRPDRRRLRAAARQFLDAIGEKDLRGDQKR
                     TPERVAEAWADEILSGYAVDPRRVLGRGFPARDRDLVIVRDIPFTSICVHHLLPFSGT
                     AHVGYLPGERLAGLSKIARVVDALSRRLQLQERLTRQVTSVVMEGLAPLGVACRMEAE
                     HLCMTVRGARKRGARVVTTAYDGAFRSRPALRSEFLRLSGNSPK"
     gene            complement(2164..3327)
                     /locus_tag="VFD06_07020"
     CDS             complement(2164..3327)
                     /locus_tag="VFD06_07020"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZN03325.1"
                     /translation="MSAGTGRIDRLECGRCGRARPFGPPAATMCLCGGTLLARYDLAA
                     GEVMRARVAGRPPDLWRYAEVLPPVEAVSLGEGFTPLLDAPRLGAALGMERLLVKDES
                     QNPTGSFKARGMTVAVSVALAHGVKVAAAPSAGNAGSALAAYGARAGMEVRLYLPEST
                     PSPLIAEARRLGARVTLVPGSIADAGKAMRAALGPPAENGWFDFSTLREPFRVEGKKT
                     MGYELFEQLDGRLPDVILYPTGGGTGLIGMWKAFDEMQALGWIGTTRPRMVSVQSQGC
                     APIPRAFAAGTETAEPWSDPVTVAWGLRVPGSLGDALMLRALRESRGDAVAVSDEDLL
                     AGAVRMATLEGIDACPEGGACVAALQAMLAGGRVGRGDLVVLFNTGSGIKYAA"
     gene            complement(3324..>3898)
                     /locus_tag="VFD06_07025"
     CDS             complement(3324..>3898)
                     /locus_tag="VFD06_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN03326.1"
                     /translation="ALLGVAIYFMQPFLSDRVLGWLAIAFALGAGVWLALIEGTKMQA
                     AWFRPLRLLVGALVVVAGAWLALPLVRAREEVPWLPYSDGALAEARSAGRPVLIDFFA
                     VWCAPCRELDRYTYVDPKVLDALDRFVLLKADLTNEESPEVQSLRDRYEVYGVPTVVM
                     IDSAGVDRKDLRLTGFEPPQPFLRRLEQVR"
BASE COUNT          585 a         1449 c         1337 g          527 t
ORIGIN      
        1 cgcgacggct cgtgggtcgg gcgcagcgtg ttgtcgggga acggatcgaa gatcccttcg
       61 aggatcagct cggcgtaggc gcgccgcgcc tcctccggca ccaggtcacg atcgcggaac
      121 gccaggaggt acggcaggtc gcgggcgtcg agggcgaggc gcatcttctc gtcccagccg
      181 agcgagcgcg tcaggtagac gacatagtcg tgcagctgca ggcggttgtc ccggagcccc
      241 tcgtggtccg gcttcttgcc gatctgcccg agcgtgtagg tggtccagcg ctccgcctcc
      301 gaaaccttgc agcgatcctg gagccaggag cggtcgagcg cctcgtttcc gacgatcccg
      361 agcaggcgca tcatctggat ctcctcgttg acgcgcgcgc cgtcctcgag cgtcaccggc
      421 tcgacccact caacccccgt cagcgcgtgc ccctccgcct ccgcctcggg atagcactcg
      481 acccccttga gatacgaccc cttctcttcc gggaagatca gggcgccgtc ctcggtgtgc
      541 ccgccgcacg tcgaggtgta cagggcgttg atcggccggc cgtcctggac ggcgacgagg
      601 ccccgcgtct cctcgaccgc ctggttggtg agcggatgct cggtgccggc ccccttgtac
      661 acctggcact gcgccgagtc gcacacgtcg tagccgctgt cggcgaactg gccgaggttc
      721 gagacgatgt aggtgcgcgc cgccaccgcc tgcgccttga gcgcctcgag ctccggatag
      781 accccgggcc ccatttcgtt cgggacgacg ccgcgcaggt actcctcgag ctcgaggacg
      841 ttgatcaccc ggatccgccc ggcccggtcc acccgcactt cgagggtgcc acggtacggg
      901 gtcgcgtcca ccttcaacca ggcgccgctt ctcgccggct gcaacaggac gcggtcggtc
      961 gcggccagga agtcgtgcca gcgctcgtcc acgaggcgca cgcggcggcg gccggcggtc
     1021 tccggctcgt cctcgacgat ccacagctcg ttgtacccct cgtcggcgag ccgcttcacg
     1081 agggtcgccg cctcctcgcg ggtgcggaat tccccggcgc gcacccgcca cgagcggcgg
     1141 tccgggtggt aggcgacgac cactttctcc gccgggagca ggctctcgat ggcggccttc
     1201 ttctggttcg ctccgtcctc ggtggcgaac gatccgatct gcacccggta caggacgagc
     1261 ggccccttcc cctgcacctc ggcgacgaac gtcagtccgg gatcgaacac cttgcgccag
     1321 atcggagtgc gcttcaccgg atccagcacg atcaccccgc cgtcggcggt gatgcgcgag
     1381 cggaccgagt cggtgctcag accgatgcgc accaaccgcg cctgcggtgc ggccgcggcc
     1441 ggcgccgccg agaacggggc cagggagagg agcgccgccg gtccgagcat cgccaggaac
     1501 gccgaagccc gccacgccgt gcggctcatc gcggagcgcc ggccgacgcg gctcatttcg
     1561 gcgagttccc cgaaaggcgc agaaactccg agcgcagcgc cgggcgggag cggaaggccc
     1621 cgtcgtacgc cgtcgtcacg accctcgccc cgcgtttgcg ggcgccgcgc actgtcatgc
     1681 agagatgctc cgcctccatc cggcaggcga cccccagggg cgccagcccc tccatcacga
     1741 ccgaggtcac ctgtcgggtc agcctctctt gaagttggag ccggcgcgac agggcgtcaa
     1801 ccacgcgggc gattttcgaa agaccggcga gccgctctcc cggcagataa ccgacatggg
     1861 cggtcccgga gaacggcagc aggtggtgga cgcagatcga cgtgaagggg atgtcacgca
     1921 cgatgacgag atcgcgatca cgcgccggaa acccacgccc gaggacacga cgcggatcga
     1981 cggcgtaccc ggagaggatc tcgtcggccc aggcctcggc gacccgctcc ggcgtgcgct
     2041 tctggtcgcc gcgcaggtcc ttttcgccga tcgcgtcgag gaactgacgc gccgcggcac
     2101 gcaggcgccg ccggtcgggg cgggagggtg ggacgcgtcc cgccttgccg cttcgtcgtc
     2161 ccttcatgcg gcgtacttga tcccgctgcc ggtattgaac aggacgacga ggtcgccacg
     2221 cccgacccgc ccgccggcga gcatcgcctg aagcgccgcg acgcacgccc ccccttcggg
     2281 gcaggcgtcg atcccttcga gggtcgccat gcgcaccgcc ccggccagga gatcctcgtc
     2341 ggacaccgcc accgcgtcgc cgcggctctc ccggagcgcc cggagcatca gggcgtcccc
     2401 gaggcttccc ggcacccgca gcccccaggc gaccgtcacc ggatcgctcc acggctcggc
     2461 ggtctccgtc cccgccgcga aggcgcgcgg gatcggcgcg cagccctgcg actgcaccga
     2521 gaccatgcgc ggccgcgtag taccgatcca gccgagcgcc tgcatctcgt cgaacgcctt
     2581 ccacatgccg atgaggccgg tcccgccccc cgtgggatag aggatgacgt cgggaagacg
     2641 tccgtccagc tgctcgaaca gctcgtaccc catggtcttc ttcccttcca cccggaacgg
     2701 ctcgcgcagc gtcgagaagt cgaaccagcc gttttcagcg gggggcccga gggcggcgcg
     2761 catcgccttg ccggcgtcgg cgatcgatcc cgggacgagc gtgacgcggg cgccgaggcg
     2821 gcgcgcctcg gcgatgaggg gggacggcgt ggactcgggc aggtacagcc gtacctccat
     2881 cccggcgcgc gcgccgtagg cggcgagcgc gctgccggcg ttgccggccg agggcgcggc
     2941 ggcgaccttc acgccgtgcg ccagggcgac cgacaccgcc accgtcatgc cgcgcgcctt
     3001 gaacgagccg gtcggatttt gcgactcgtc cttgacgagg aggcgttcca tcccgagcgc
     3061 cgcaccgagc cgcggcgcgt cgaggagcgg cgtgaaccct tcgcccaggc tcaccgcctc
     3121 gacgggaggc aggacctcgg cgtagcgcca cagatccgga gggcggccgg ccacgcgcgc
     3181 ccgcatcacc tcgccggccg cgaggtcgta gcgggccagg agcgtcccgc cgcagaggca
     3241 catcgtcgcg gccggagggc cgaaggggcg ggcgcggccg cagcggccgc actccagtcg
     3301 atcgatccgg ccggtccccg cgctcaccgg acctgttcga gacgcctgag gaacggttgc
     3361 ggaggctcga aaccggtcag gcggaggtcc ttccggtcca ccccggccga gtcgatcatc
     3421 acgacggtcg gcaccccgta cacctcatac cgatcgcgca gcgactgcac ttcgggagac
     3481 tcctcgttgg tcaggtcggc cttgagcagg acgaagcgat cgagggcgtc gagcacttta
     3541 gggtcgacgt aggtgtagcg gtcgagctcc cggcaggggg cgcaccagac tgcgaagaaa
     3601 tcgatcagca ccggccggcc ggcggagcgc gcttcggcga gcgccccgtc ggaatagggg
     3661 agccacggca cctcttcgcg cgcccgcacc agcggcagcg ccagccacgc gccggcgacg
     3721 accacgagcg cgccgaccag caggcgcagc ggccggaacc aggccgcctg catcttcgtc
     3781 ccctcgatca gggcgagcca aacgccggcc cccagagcga acgcgatcgc cagccacccg
     3841 agcacccggt cgctcaggaa cggctgcatg aaataaatcg ccacgcccag gagcgcca
//
LOCUS       DASRIQ010000289         7157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_895415, whole genome shotgun sequence.
ACCESSION   DASRIQ010000289 DASRIQ010000000
VERSION     DASRIQ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895415"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFD06_07030"
     CDS             complement(<1..267)
                     /locus_tag="VFD06_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03327.1"
                     /translation="MAMAAGAISVALLAEASAADTVRLRYGLKPQAAYEQTIGINLVM
                     TIDPASLPAPLLALLQSMAGDVKQEVTSKARLQTKTRNADGSLPL"
     gene            425..509
                     /locus_tag="VFD06_07035"
     tRNA            425..509
                     /locus_tag="VFD06_07035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:459..461,aa:Leu,seq:tag)
     gene            655..1965
                     /gene="tig"
                     /locus_tag="VFD06_07040"
     CDS             655..1965
                     /gene="tig"
                     /locus_tag="VFD06_07040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZN03328.1"
                     /translation="MPANDNDAMTVVVNELERCKRGLEVAIPHVRVADEMDRAFREYA
                     HHARVPGFRKGHIPMDVVRRRFGKEVRDEVIGRIVRTEALRILEERRLDPVEPPVLEE
                     VTYEDGQPLKFRATFEVRPAIEVSDYRGLSVTVPKHAVTDEMVESSVRGLAERAARLE
                     AISGRPVQKGDFIVGTLSCRFVKGKGKNLKDEPLLLEAGSEENHPDFNAAILGMEAGT
                     TRTFEVDYPADYNAVSLRNSIVEYTLVLQEIKKKVVPPLDDSLAKEIGAFASMEEMRA
                     RVRSEIERRAALAERDEARQRLLADLVRRHPMEVPDAMVEAQIDARVEALAREMIARG
                     VDPMKAEIDWPQERERARPGATDAVRAMLLLDAIAAREKIEASEDDLNEVLKEEARRH
                     NSSVASVKEKLGQSARLAGIRRQIVREKSLDFVLRDATITPEVR"
     gene            1972..2556
                     /gene="clpP"
                     /locus_tag="VFD06_07045"
     CDS             1972..2556
                     /gene="clpP"
                     /locus_tag="VFD06_07045"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZN03329.1"
                     /translation="MPLIPMVVEQTNRGERAYDIYSRLLKDNIVFIGHAIDEHLSNVV
                     IAQLLFLEAEDPEKDISIYINSPGGSVTAGLAIYDTMQFIRPDISTICIGEAASMAAV
                     LLAAGTKKKRFALPNSRVLLHQPMGGFSGQATDVDIHAKEILRVRQRLNEILAHHTGQ
                     TLERIHRDTDRDFILTGGSAMEYGVIDQVISHRD"
     gene            2606..3844
                     /gene="clpX"
                     /locus_tag="VFD06_07050"
     CDS             2606..3844
                     /gene="clpX"
                     /locus_tag="VFD06_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZN03330.1"
                     /translation="MAGKRGDGESLRCSFCNKDQRDVKKLIAGPTVYICDECVDICLD
                     IIAEEREPDEPKAKVKLPKPMEIREFLDEYVVGQEKAKKKLAVAVYNHYKRNELAKRR
                     GEVEVQKSNILLIGPTGTGKTLLAQTLAKLLSVPFAIVDATTLTEAGYVGEDVENILL
                     RLLQAAGNDIEKAKRGIIYIDEVDKISRKSDSPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQDFIPMDTTNILFICGGAFVGLDTIIEQRIGKKAMGFKADIKRKTDR
                     TVLEVLEQVQPADLIKYGLIPEFVGRLPVVGVLGELDREALVKILTEPRNALVKQYQR
                     SFEFENVTLTFTRDALDAIAEQALLRKIGARGLRLIMEDLMLDLMYQLPSRTDIHECV
                     ITKEVVENKMAPIMLMEKAG"
     gene            3859..6264
                     /gene="lon"
                     /locus_tag="VFD06_07055"
     CDS             3859..6264
                     /gene="lon"
                     /locus_tag="VFD06_07055"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZN03331.1"
                     /translation="MLVKLEDSTETLPLVPLRDVVVFPYTMIPFVVGRRSSLLAVERA
                     LQGDKRIFLATQRDAKIDDPKPDEINVIGTVANIVQSLKLPNGNIKLLVEGTSRGRCQ
                     RLEIVDGCLIASLTLIDLKAELTPEVETQMGKVVSLFEKYVKLSPNMPYETMISTIRV
                     SEPGRLTDTIAAHLQVEVEAKQSLLEVFNPVERLSLLSGILDAEIEKLRVDRRIHQRV
                     KKQMEKAQKEYYLNEKMKAIQQELGRKDDKLNEVEELKQRIQAAKMPKEALEKAMQEL
                     KRLEVMPPVSAEATVSRNYLDWLLSVPWHKRTKEIRDLDFAEKVLNEDHHGLEKVKER
                     ILEFLAVRRLVGNKHMKGSILCLVGPPGVGKTSLAQSVAKATGRKFVRVSLGGVRDEA
                     EIRGHRRTYIGAFPGQILQMMKKAGTKNPVFLLDEVDKMSMDFRGDPSSALLEVLDPE
                     QNGTFLDHYLDTEFDLSQVMFICTANVLHPIPPALKDRMEVLRLAGYTLIEKEAIARR
                     FLIPKQLKAHGLDPAKFQVEDAALKDAIEKYTREAGVRNLERELASVCRKLARRVVRE
                     GSEFTFTVKSEHLSDFLGIPRYRPTTSDKLPEIGVATGLAWTEVGGEILLTEATLMKG
                     RGKLMLTGKLGDVMQESAQAAMSFVRSRAQMLGIDEDFHRKYDIHVHVPEGAIPKDGP
                     SAGITMATALASLLTKTPVRGDVAMTGEITLRGKVLPIGGVKEKILAAHRLGVRTVIL
                     PRDNEKDLADVPEDVQKALEFRLVETMDDVLQIALERESAPKVDKEYNKDLDKGLPGS
                     VTH"
     gene            6376..7077
                     /gene="yihA"
                     /locus_tag="VFD06_07060"
     CDS             6376..7077
                     /gene="yihA"
                     /locus_tag="VFD06_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZN03332.1"
                     /translation="MLIRETELFASASSPDRFPRDGAPQIAFMGRSNVGKSSLINRLL
                     GRKGLARTSKTPGRTRALHFFLVNGRCYFVDLPGYGYAKVPRPMREEWKGFVESYLEG
                     RGPDLAVVLTDARRPPTPLDRELVDWLRAAGVPHTVVLTKADKLSRRELLPASRQAGA
                     ALGLPSDLPPLPVSAVSGDGVPALWRTIDDACSRRRSPEPVPSSDDDGASHRPNPRHQ
                     ALTTPEIPASRRMRP"
BASE COUNT         1342 a         2364 c         2426 g         1025 t
ORIGIN      
        1 cagggggagg ctgccgtcgg cgttccgggt cttcgtctgc agccgtgcct tcgacgtaac
       61 ctcctgcttg acgtctccgg ccatcgactg gagcagggcc aggagcgggg ccgggagcga
      121 agcgggatcg atggtcatga cgaggttgat gccgatcgtc tgttcgtacg ccgcctgcgg
      181 cttgagccca tagcgcaggc gaacggtgtc ggccgcggag gcctcggcca gaagcgcgac
      241 cgagatcgcc cctgcggcca tcgccaagcg cgccgcatgg ccgcggcggg ccggacacgg
      301 agcggtgtgg gtgctcacag gcgtcggagc ctagcccacg cccccgtgga ggtcaaggtt
      361 cgccggggcc ctggcggcgc gatcggtctc gggggcctgg tgttagagtg tcgccgcggc
      421 ggccgcgaaa gtggcggaac cggcagacgc gccggactta ggatccggtg gggcaacccg
      481 tgagggttcg aatccctcct ttcgcaccac gccgcgggga cgcaggctcc tgggggcttg
      541 cggggggcga ctcggtctgc tagagtccca cgcgtgtaga aaggaagttc gcaacgcgtc
      601 cgccgtggtt gacggacccc gcctgtagcg aatcccaaga gcctggcgac gacgatgccc
      661 gcgaacgaca acgacgccat gaccgtggtc gtgaatgaac tggagcgctg caagcgcgga
      721 ctcgaagtgg ccattccgca cgtgcgggtg gccgacgaga tggaccgcgc gttccgggag
      781 tatgcccacc atgcccgcgt ccccggcttc cgcaaggggc acatcccgat ggacgtcgtg
      841 cgccggcgct tcggcaagga ggtccgcgac gaggtgatcg gccgcatcgt gcgcaccgag
      901 gcgctccgga tcctcgagga gcgaaggctc gatccggtcg agccgccggt cctcgaggag
      961 gtcacctacg aggacggcca gccgctcaag ttccgcgcca ccttcgaggt gcggccggcg
     1021 atcgaggtgt ccgactaccg cggcctgagc gtgaccgttc cgaagcacgc ggtgaccgac
     1081 gagatggtgg aatcctcggt gcggggcctg gcggagcggg cggcgcgtct cgaggcgatc
     1141 tccgggcgcc ccgtgcagaa gggggacttc atcgtcggca ccctctcctg ccggttcgtg
     1201 aaggggaaag ggaagaacct caaggacgag ccgctgctgc tggaggccgg atcggaggag
     1261 aaccatcccg acttcaacgc cgccatcctc gggatggagg cgggcacgac gcgcaccttc
     1321 gaggtcgact acccggccga ttacaatgcc gtcagcctgc ggaactccat cgtcgagtac
     1381 acgctcgtac tccaggaaat caagaagaag gtcgtccctc ccctcgacga cagcctcgcg
     1441 aaggagatcg gggctttcgc ctcgatggag gagatgcggg cgcgggtgcg cagcgagatc
     1501 gagcggcgcg ccgccctcgc cgagcgcgac gaggctcgcc agaggctcct cgccgacctc
     1561 gtgcggcgcc acccgatgga ggtccccgac gcgatggtcg aggcgcagat cgacgcgcgc
     1621 gtcgaggccc tggcccgcga gatgatcgcg cgcggtgtcg acccgatgaa ggcggagatc
     1681 gactggccgc aggagcgcga gcgggcccgc ccgggcgcca ccgacgcggt gcgcgccatg
     1741 ctcctgctcg atgcgatcgc cgcccgcgag aagatcgagg cgagcgagga cgatctcaac
     1801 gaggtgctca aggaggaagc gcgccgccac aactcgagcg tcgcgtctgt caaggagaaa
     1861 ctcggccaaa gtgcgcgtct ggcgggcatt cggaggcaga tcgtcaggga aaagtcgctt
     1921 gacttcgtgc tgcgtgatgc tactattacg cccgaggtga gatagccccc catgcccctc
     1981 atccccatgg tcgtcgaaca aacaaaccgt ggggaacgcg cctacgacat ctactcgcga
     2041 ctcctcaaag acaacatcgt gttcatcggc cacgccatcg acgagcacct cagcaacgtg
     2101 gtgatcgccc agctgctgtt cctcgaagcc gaggatccgg agaaggacat ctcgatctac
     2161 atcaacagcc cgggcggctc ggtgaccgcg gggctggcga tctacgacac gatgcagttc
     2221 atccgtccgg acatctcgac catctgcatc ggcgaggccg ccagcatggc cgccgtcctc
     2281 ctcgcggccg ggacgaagaa gaagcgcttc gcgctgccga actcgcgcgt gctgctgcac
     2341 cagccgatgg gcgggttctc gggacaggcg accgacgtcg acatccatgc gaaggagatc
     2401 ctccgcgtcc ggcagaggct caacgagatc ctggcccacc acaccggcca gacgctcgag
     2461 cgcatccacc gggacacgga ccgcgacttc atcctgaccg ggggctccgc gatggagtac
     2521 ggcgtcatcg atcaggtcat ttcgcaccgc gattaggcga ggccccgaga tctcgatgct
     2581 cgggtcgacg aggagagcat aggccatggc cgggaagcgt ggtgacggag aatccctccg
     2641 ctgcagcttc tgcaacaagg atcaacgcga cgtcaagaag ctgatcgcgg gtccgaccgt
     2701 ctacatctgc gacgagtgcg tcgacatctg cctcgacatc atcgccgagg agcgcgagcc
     2761 ggacgaaccc aaggccaagg tcaagctccc gaagccgatg gagatccgcg agttcctcga
     2821 cgagtacgtc gtcggccagg agaaggcgaa gaagaagctg gcggtcgccg tctacaacca
     2881 ctacaagcgc aacgagctgg cgaagcggcg cggcgaggtc gaggtgcaga agagcaacat
     2941 cctgctcatc ggcccgaccg gcaccggcaa gacgctcctg gcccagacgc tggccaagct
     3001 cctctccgtg ccgttcgcga tcgtcgacgc gacgacgctg accgaggccg gctacgtcgg
     3061 cgaggacgtc gaaaatatcc tgttacggct tctccaggcg gccggcaacg acatcgagaa
     3121 ggcgaagcgc ggcatcatct acatcgacga agtggacaag atcagccgca agagcgactc
     3181 gccgtcgatc acccgcgacg tgtccggcga gggggtgcag caggcgctcc tgaagatcct
     3241 cgaggggacg gtcgccaacg tgccgccgca gggcggacgc aagcacccgc accaggactt
     3301 catcccgatg gacacgacga acatcctgtt catctgcggc ggggccttcg tcgggctcga
     3361 caccatcatc gagcagcgca tcggcaagaa ggcgatggga ttcaaggccg acatcaagcg
     3421 gaagaccgat cgcacggtgc tcgaggtgct cgagcaggtg cagccggccg acctgatcaa
     3481 gtacggactc atcccggagt tcgtcggccg cctgccggtc gtcggcgtgc tcggagagct
     3541 ggaccgcgag gcgctcgtga agatcctgac cgagccgcgc aacgcgctgg tgaagcagta
     3601 ccagcgctcc ttcgagttcg agaacgtgac gctgaccttc acccgcgacg cgctcgatgc
     3661 gattgccgaa caggcgctgc tgcgcaagat cggcgcgcgc ggcctgcggc tgatcatgga
     3721 agacctgatg ctcgacctga tgtaccagct cccgtcccgc accgacatcc acgaatgcgt
     3781 catcacgaag gaagtcgtcg agaataaaat ggcgcccatc atgttgatgg agaaggccgg
     3841 ctgagcgccg gatccccgat gctcgtaaaa ctcgaagatt cgaccgaaac cctgcccctc
     3901 gtccccctgc gggacgtggt ggtcttcccg tacaccatga tccccttcgt cgtcgggcgc
     3961 cgctcttcgc tcctggcggt cgagcgggcg ctccaggggg acaagcggat cttcctggcg
     4021 acgcagcgcg acgcgaagat cgacgacccc aagccggacg agattaacgt catcggcacg
     4081 gtggcgaaca tcgtccagag cctgaagctg ccgaacggca acatcaagct cctggtcgaa
     4141 ggaacgtcgc gcggccgctg ccagcggctg gagatcgttg acgggtgcct catcgcgtcg
     4201 ctcacgctca tcgacctcaa ggcggagctc acgcccgagg tcgagacgca gatggggaag
     4261 gtggtctcgc tcttcgagaa gtacgtgaag ctgtccccga acatgcctta cgagacgatg
     4321 atctcgacga tccgggtctc cgagccgggc cgcctcaccg acaccatcgc cgcccacctc
     4381 caggtcgagg tggaagcgaa gcagagcctg ctcgaggtgt tcaacccggt cgagcgtctg
     4441 tcgctcctct ccggcatcct cgacgccgag atcgagaagc tgcgcgtcga ccgccgcatc
     4501 catcagcgcg tcaagaagca gatggagaag gcgcagaagg agtactacct caacgagaag
     4561 atgaaggcga tccagcagga gctgggccgc aaggacgaca agctcaacga ggtcgaggaa
     4621 ctcaagcagc gcatccaggc ggcgaagatg ccaaaggagg cgctcgagaa ggcgatgcag
     4681 gagctgaagc gcctcgaggt gatgcctccg gtcagcgccg aggcgacggt gtcacgcaac
     4741 tacctcgatt ggctgctgtc ggtgccgtgg cacaagcgca cgaaggagat ccgcgacttg
     4801 gacttcgccg aaaaggtcct caacgaggac catcacgggc tggagaaggt caaggagcgc
     4861 atcctcgagt tcctggcggt gcgccgcctc gtcggcaaca agcacatgaa gggctcgatc
     4921 ctgtgcctcg tcggcccgcc cggcgtgggg aagacctccc tggcgcagtc ggtcgccaag
     4981 gcgaccggcc gcaagttcgt gcgcgtctcg ctcggcgggg tgcgcgacga ggcggagatc
     5041 cgcgggcacc ggcgcaccta catcggcgcc ttcccgggcc agatcctgca gatgatgaag
     5101 aaggccggga cgaagaaccc ggtcttcctg ctcgacgagg tggacaagat gagcatggac
     5161 ttccggggcg acccgtcgtc cgcgctcctc gaggtgctcg atccggagca gaacggcacc
     5221 ttcctggatc actacctcga caccgagttc gacctgagcc aggtgatgtt catctgcacg
     5281 gcgaacgtcc tgcacccgat cccgccggcc ctcaaggacc ggatggaggt gctgcggctg
     5341 gccggctaca cgctcatcga gaaggaggcg atcgcccgcc gcttcctgat cccgaagcag
     5401 ctcaaggcgc acgggctcga ccctgcgaag ttccaggtcg aggacgccgc cctcaaggac
     5461 gccatcgaga agtacacgcg cgaagccggg gtgcgcaatc tcgagcgcga gctcgcctcc
     5521 gtctgccgca agctggcccg gcgcgtggtg cgagagggtt cggagttcac cttcaccgtg
     5581 aagtccgagc acctgagcga cttcctcggg atcccgaggt accggccgac cacgagcgac
     5641 aagctgccgg agatcggggt tgccaccggg ctggcgtgga ccgaggtcgg aggggagatc
     5701 ctcctcaccg aggcgaccct gatgaaaggg cgcggcaagc tgatgctcac cggcaagctc
     5761 ggcgacgtca tgcaggagtc ggcgcaggcg gcgatgtcgt tcgtgcggtc ccgcgcccag
     5821 atgctcggca tcgacgagga cttccaccgc aagtacgaca tccacgtgca cgtgccggag
     5881 ggggccatcc cgaaggacgg cccctcggcc gggatcacca tggccacggc gctggcgtcc
     5941 ctcctcacca agacaccggt gcgcggcgac gtcgcgatga ccggcgagat caccctgcgc
     6001 ggcaaggtcc tgccgatcgg cggcgtcaag gagaagatcc tggcggcgca ccgcctcggc
     6061 gtgcgcaccg tcatcctccc ccgcgacaac gagaaggacc tggccgacgt ccccgaggac
     6121 gtgcagaagg cgctggagtt ccgcctggtg gagacaatgg acgacgtgct gcagatcgca
     6181 ctggagcgcg agtccgcccc caaggtggac aaggaataca ataaggacct cgacaagggc
     6241 cttccgggct cagtcacgca ctgatttccg atacgcgcga gcgcggccgt cgcgctccgc
     6301 tccctgggat gacgggacgg tctccggcgc ggtgttccgg cgcccggcgg cgccccggcc
     6361 cgatgcttcc gaaccatgct gatccgagaa accgaacttt tcgccagcgc ctcttccccc
     6421 gatcgcttcc cgcgcgacgg ggcgccgcag atcgccttca tggggcgctc gaacgtgggg
     6481 aagtcgagcc tgatcaacag gctcctcgga cggaagggac tggccaggac cagcaagacc
     6541 ccgggaagga cccgagccct tcacttcttc ctggtcaacg ggaggtgcta cttcgtggat
     6601 ctcccgggct acgggtacgc caaggtgccg cggccgatgc gcgaggaatg gaaggggttc
     6661 gtcgagtcct acctggaagg gcgggggccg gacctggcgg tggtgctcac cgacgcgcgg
     6721 cgccctccga cgcccctcga ccgggagctc gtcgactggc tccgggccgc gggcgtgccc
     6781 catacggtcg tgctgaccaa ggcggacaag ctgtcccgga gggagctcct gccggcctcc
     6841 cggcaggccg gcgccgcgct gggcctgcca tccgacctgc cgccgctccc ggtctcggcg
     6901 gtgtcgggtg acggcgtgcc ggcgctgtgg cggaccatcg acgacgcctg ctcgcgaagg
     6961 cgatccccgg aacctgtccc gtcgtccgac gacgacgggg cttcgcatag accgaaccct
     7021 cgacaccaag cgctgacgac gccggagatc ccggccagca gaaggatgcg accgtgaacg
     7081 gaaaagaaac ggaccgtgat tccagaggca accgcgagcc ccgcccgccc cgcgggccgg
     7141 gccgcgagca gggaggg
//
LOCUS       DASRIQ010000290         5028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_895517, whole genome shotgun sequence.
ACCESSION   DASRIQ010000290 DASRIQ010000000
VERSION     DASRIQ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895517"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFD06_07065"
     CDS             <1..339
                     /locus_tag="VFD06_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose 5-phosphate 3-epimerase"
                     /protein_id="HZN03333.1"
                     /translation="KGEAEHLAPASVRDALFPEGTAARLFVGHTRPEFLLGLVAPLHT
                     GRGRTAALGFLGLGGTLTTSGMLFVNRCTWAHILQESARLAGLPRESLLSRGEMDVLD
                     GRIAPEGIVM"
     gene            336..860
                     /locus_tag="VFD06_07070"
     CDS             336..860
                     /locus_tag="VFD06_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HZN03334.1"
                     /translation="MIRRMWRAAVLDSELYDEVESDPRLGWEALWIVLLVSVAGGIGA
                     GWPHPGGVAAGAAAMFVGWLAWAAVTNWLGTRVFPEPQTECDFPEMLRTLGFAASPGL
                     LAVLGVVSETRVAAFACATLWTLAATIVAVREALDYGSTGRALVVCAIGWVLQLAAVV
                     ATLALFAVGARPAL"
     gene            complement(889..1311)
                     /locus_tag="VFD06_07075"
     CDS             complement(889..1311)
                     /locus_tag="VFD06_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZN03335.1"
                     /translation="MVDMKDIEGQVRGRLERDLGRVTHWVAALRAGWRPEELEAGGDN
                     TPLSEAADAAQVVEERETGTQLLDRLVHRGEEIRGALRRLDEGTYGFCVSCSRTIHPK
                     RLMARPEASLCLDCQTERERHHPDRVTREPVLDASPLD"
     gene            complement(1426..2436)
                     /locus_tag="VFD06_07080"
     CDS             complement(1426..2436)
                     /locus_tag="VFD06_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZN03336.1"
                     /translation="MDVLSDVLKAVTLEGAVFYNAEFSAPWGFRAPHSRTVRPLLSPG
                     VGHVIMFHLLTEGRGSARLDEGEVLPLEAGDIVVFPHGDPHVVENGGWRPSAKKKGDV
                     QRVMSRGLRLARGGGGGEVTRFVCGYMACEPRLGRLVLAGLPPVFKVHIRDDAAGRWL
                     ESSIRFSVDAAAQAGPGGEAVLAKLSEALFVETLRRYVAQWPEGRTGWLAGARDPEVG
                     RVLALMHRRPAHPWSLADLAREAGLSRSVLAERFRHFLHQPPMAYLTGWRLQLGARIL
                     GSSSRSVADVAAEVGYESEAAFNRAFKRSFGVPPARFRNQSRSASFPGNPATPKSSAR
                     RR"
     gene            2632..4020
                     /locus_tag="VFD06_07085"
     CDS             2632..4020
                     /locus_tag="VFD06_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011865287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN03337.1"
                     /translation="MKAVSEAAIRELKNGFAGEVILPGDAEYDEARRIWNAMIDRRPA
                     ILARCAETGDVVRAVRAARKLGLRLAIRGGGHNIGGLALCDDGLVVDLSRMRRAQVNA
                     PARRVTIEGGATLAELDAATQAHGLATPVGINSTTGLAGLTLGGGFGWLSRRHGLTID
                     NLESAEVVTAEGEVVRASASEHPDLFWALRGGGGNFGVVTRFEYRLHPVGPEVLAGLI
                     VYPLSDGESVLRQYGEFAAQAPDDLTVWAVLRNAPPLPFLPPAVHGTGIVALAVFYGG
                     DPARGAALVEPLRKFGPLAGEHVGVQPYAAWQQAFDPLLTAGARNYWKSHNLLRLDDR
                     LLDAAVGAARKLPSSGSEIFLAAVGGAMSRLAQDSAAYPHREAQFVVNVHGRWDTAEE
                     DARGISWARAFFNATSEFAAGGAYVNFMTDDEGADRVRAAYGANYDRLARVKRAWDPE
                     NVFSVNQNIRPA"
     gene            complement(4026..>5028)
                     /locus_tag="VFD06_07090"
     CDS             complement(4026..>5028)
                     /locus_tag="VFD06_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HZN03338.1"
                     /translation="RHATDRPLRRALRTVLLACPLFLTAPAAELPVAEAGAEAGAEPG
                     GAVAGPARAGAEPSRGPAAGPKDGPMSPTAAETSKVSIQAIRYATIPGFPLAGLMMGA
                     PPDERIDIAMVIWLVRLPNHAVLFDTGFHRDRWLKEFTVADYLRPDEAVALAGVRADQ
                     VTDVVISHAHWDHLGGVDLFPNARVWIQKQEYAYYTGDAWQPGGRSGGVDPEDVACLL
                     RLNTAGRLRLVDGDDVEILPGLRVYTGARHTFASQYLRVETPAGPFVLASDNCYLDRN
                     LRERVPSATFDEADRPKNLAAQRRMIALAGEAERVIPGHDPRQFERFPSEGRVARIH"
BASE COUNT          696 a         1784 c         1852 g          696 t
ORIGIN      
        1 aagggggagg cggagcacct ggcccccgcc tccgtgcggg acgccctctt tcccgagggg
       61 acggcggcgc gcctgttcgt cggccacacg cgtcccgaat tcctcctcgg cctcgtcgcg
      121 ccgctccaca ccggacgcgg gcggaccgcg gcgctcggct tcctggggct cggcgggacc
      181 cttacgacct ccggcatgct cttcgtgaac cgctgcacct gggcgcacat cctgcaggaa
      241 tcggcgcgcc tcgccggcct gccacgcgag agcctgctct cccggggcga gatggacgtc
      301 ctggacgggc gcatcgcccc cgaagggatc gtcatgtgat ccgccggatg tggcgcgccg
      361 cggtgctcga ttcggagctg tacgacgagg tcgagtcgga cccgcggctc ggctgggagg
      421 cgctgtggat cgtgctgctc gtcagcgtgg cgggcgggat cggcgccggc tggcctcacc
      481 cgggaggagt ggcggcgggg gcggcggcga tgttcgtggg atggctcgcg tgggcggcgg
      541 tcacgaactg gctcgggacg cgggtctttc cggagccgca gaccgagtgc gactttcccg
      601 aaatgctccg cacgctcggc ttcgccgcgt cgcccgggct cctggcggtg ctcggagtgg
      661 tttcggaaac gagagtcgcc gccttcgcct gcgcgaccct ctggacgctg gcggccacca
      721 tcgtggcggt gcgggaggcg ctggactacg gatccacggg acgcgcgctc gtcgtctgcg
      781 cgatcggctg ggtgctgcag ctggccgccg tcgtcgcgac cctggcgctg ttcgccgtcg
      841 gcgcccgacc ggccctctga atcctgcggg gcggcgcggc gggaccggtc agtccagcgg
      901 ggaagcgtcc agcaccggct cgcgcgtgac ccgatcggga tggtgccgct cgcgctccgt
      961 ctggcagtcg agacacaacg atgcctcggg acgcgccatc aggcgctttg ggtggatcgt
     1021 ccgactacag gatacgcaga atccgtacgt tccctcgtcg aggcgtcgca gcgcgccccg
     1081 gatctcctcg ccgcggtgca ccagccggtc gagaagctgc gtgccggtct cccgctcctc
     1141 gaccacctgc gcggcgtcgg ccgcttcgct cagcggcgtg ttgtctccgc ccgcttccag
     1201 ctcctcgggc cgccagccgg cccgcagcgc ggccacccag tgggtgaccc gacccaggtc
     1261 cctttcgagc cgaccccgca cctggccttc gatgtccttc atgtccacca tgttccctcc
     1321 ttcctcagcg tgcgcggcac gacgctgcgc gacgtttagg ctaaacctcc agcaacgcgt
     1381 gtgccacggg caggaccacc gaccggcgca gcggcctggc caggtctagc gccggcgggc
     1441 ggatgacttc ggcgttgcgg gattcccggg gaaggaggcc gaccgggact ggttgcggaa
     1501 gcgtgcgggc gggacgccga aggagcgctt gaaggcgcgg ttgaaggcgg cctccgattc
     1561 gtagccgacc tccgccgcca cgtcggcgac gctgcggctg gacgatccga ggatgcgggc
     1621 gccgagctgg aggcgccagc ccgtcaggta ggccatcggc ggctggtgca gaaagtggcg
     1681 gaaacgctcc gccaggacgg accgcgacag ccccgcctcg cgtgccaggt cggcgagcga
     1741 ccagggatgg gcgggcctcc ggtgcatgag cgcgagcacc cggccgactt cgggatcacg
     1801 tgcccccgcg agccacccgg ttcgcccctc cggccactgc gccacgtagc gccggagcgt
     1861 ctcgacgaac aaggcctccg acagcttggc gagcaccgcc tcgcctccgg ggcccgcctg
     1921 ggccgcggca tcgacggaga agcggatgga gctctcgagc cagcggccgg cggcatcgtc
     1981 ccggatgtgc accttgaaca cgggcggcag ccccgccagc acgagacggc cgagccgcgg
     2041 ctcgcacgcc atgtagccgc agacgaacct ggtgacttcg cccccgcctc cgccgcgggc
     2101 gagccggagc ccgcgtgaca tcacgcgctg cacatcccct tttttcttgg cggacggccg
     2161 ccagccgccg ttctccacga cgtgcggatc gccgtgcggg aagacgacga tgtcccccgc
     2221 ctcgagcggc agcacctccc cctcgtcgag gcgggcggat ccacgccctt ccgtgagcag
     2281 gtggaacatg atgacgtggc cgacgccggg ggacaggagc ggacgaaccg tgcgcgagtg
     2341 cggcgcccgg aatccccacg gcgccgagaa ctcggcgttg tagaagaccg ccccttcgag
     2401 ggtcaccgcc ttcagcacat cggagaggac atccataggg ccggcggcgc gacgcccggc
     2461 aaagcgggcc ggacgcgcgg gcaagcggag ccgatgctaa cgtcttgcgg tcaaggacgc
     2521 cagacgcgcg ggcaagcgcc gcggaacgag gccgcgccac agtatgccgc gcgggccgga
     2581 aaccggttcg aagaccggac ggaatcccga tccccgcgaa aggagcgaac catgaaggcg
     2641 gtgagcgagg cggcgatccg ggagctcaaa aatggattcg cgggggaagt gatcctcccc
     2701 ggcgacgcgg aatacgacga agcgcggcgg atctggaacg cgatgatcga ccggcggccg
     2761 gcaatcctcg cgcgctgcgc cgaaaccggg gacgtcgtgc gcgccgtgcg cgccgcgcgc
     2821 aagctcgggc tgcgcctcgc gatccggggg ggcgggcaca acatcggcgg gctggcgctc
     2881 tgcgacgacg gcctcgtcgt cgatctctcc cgcatgcggc gcgcgcaggt gaacgccccc
     2941 gcgcggcggg tgacgatcga gggcggggcg acgctcgccg agctcgacgc cgcgacgcag
     3001 gcgcacgggc tggcgacgcc ggtcggcatc aactccacca ccggcctcgc cggcctgacg
     3061 ctcggcggcg ggttcggctg gctgagccgc cgccacggac tgaccatcga caacctggag
     3121 tcggccgagg tggtgacggc cgaaggggag gtggtccgcg cctccgcctc ggagcatccg
     3181 gatctcttct gggccctgcg cggcgggggc ggaaatttcg gggtggtcac gcgcttcgag
     3241 taccggctgc acccggtcgg cccggaggtg ctcgccggtc tcatcgtcta cccgctgtcg
     3301 gacggggagt cggtcctgcg gcagtacggg gaattcgcgg cgcaggcgcc ggacgatctc
     3361 accgtctggg cggtcctgcg caacgcgccg ccgctgccgt tcctgccgcc cgcggtgcac
     3421 ggcaccggga tcgtggcgct ggcggtgttt tacgggggcg atccggcgcg cggcgcggcg
     3481 ctcgtcgagc cgctgcgcaa gttcggcccg ctcgccggcg agcatgtcgg ggtgcagccg
     3541 tacgcagcct ggcagcaggc gttcgatccg ctgctcaccg ccggggcgcg caactactgg
     3601 aagtcgcaca atctgttgcg gctcgacgac cggctcctcg acgccgccgt gggcgccgcg
     3661 cggaagctgc cctcgtccgg aagcgagatc ttcctcgccg ccgtcggcgg cgccatgtcc
     3721 cgtctggcgc aggactccgc cgcctacccg caccgcgagg cgcagttcgt cgtgaacgtg
     3781 cacggccgct gggacaccgc ggaggaggac gcgcgcggca tcagctgggc ccgggcgttc
     3841 ttcaacgcga cctcggaatt cgcggccggc ggcgcctacg tcaacttcat gaccgacgac
     3901 gagggggcgg accgggtgcg cgccgcctac ggggccaact acgaccgtct ggcgcgggtg
     3961 aagcgcgcct gggatccgga gaacgtcttc tcggtcaacc agaacatccg cccggcctag
     4021 gctcctcagt ggatgcgcgc cacccggcct tcggacggga agcgctcgaa ctgacgggga
     4081 tcgtgtccgg ggatgacgcg ttcggcctcg cccgcgagcg cgatcatccg cctctgcgcg
     4141 gcgagattct tcggtcggtc cgcctcgtcg aaggtggcgg aggggacccg ttccctgagg
     4201 ttccggtcga ggtagcagtt gtccgacgcg aggacgaacg ggccggccgg ggtctcgacg
     4261 cgcaggtact gcgaggcgaa ggtgtggcgc gcgccggtgt agacgcgaag gccgggaagg
     4321 atctccacgt cgtcgccgtc caccagacgc agccgccccg cggtgttgag gcggagcagg
     4381 caggccacgt cctcgggatc gacgccgccg ctccggccgc ccggctgcca ggcatccccg
     4441 gtgtagtagg cgtattcctg cttctggatc cagacgcgcg cgttcgggaa gagatcgacg
     4501 cctccgaggt ggtcccaatg cgcgtgactg atgacgacgt cggtgacctg gtccgcgcgc
     4561 acccccgcga gcgccaccgc ctcgtcgggg cgcaggtagt cggcgacggt gaactccttg
     4621 agccaccggt cccgatggaa gccggtgtcg aagagcacgg cgtgattggg aaggcgcacg
     4681 agccagatca ccatggcgat gtcgatccgc tcgtcggggg gcgcacccat catcagcccc
     4741 gcgagcggga agcccggaat cgtcgcgtag cggatcgcct ggatggaaac cttcgacgtt
     4801 tcggccgccg tgggggacat tggcccgtcc ttcgggcccg cggccggccc gcgggacggc
     4861 tccgcgcccg cccgggctgg accggcgacc gcgccccccg gctcggcccc cgcctccgcc
     4921 cccgcctccg cgaccggtag ttctgcggcc ggcgccgtca ggaacagcgg acaggcgagg
     4981 aggaccgttc ggagggcgcg ccgaaggggc cgatccgtgg catgtcgg
//
LOCUS       DASRIQ010000291         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_896260, whole genome shotgun sequence.
ACCESSION   DASRIQ010000291 DASRIQ010000000
VERSION     DASRIQ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896260"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..2133
                     /locus_tag="VFD06_07095"
     CDS             16..2133
                     /locus_tag="VFD06_07095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011044998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZN03339.1"
                     /translation="MVALGAVAIAAEENAVKDNVLLAEWTGPYGGVPAFDRVRVADMK
                     PALEAAMAQNLQELEAIAKDPAPPTFENTIAAMEKTGRTYDRVITYWGLWGQNLSTPE
                     YREVQEEMAPRLAEFRSRITQNAALFARVKAVYDARTTSGLRPDQQRLVYLIYDSFAR
                     DGAALTGAAKERHAAINSRLAELYTRFANNVLADEESYVTWLTKEQTGGLPESLLKSA
                     AAAAEEKGRKGEYAITNTRSSVEPFLTYSTDRALREKVWRAFYARGGNGDEHDNNAVI
                     AEILALRNERAHLLGYPNYAAWQTENKMAKTPERATALMQEVWAAATARVKEEVADMQ
                     AVADKDKAGVTIAGWDYRFYAEKVRRARYDLDSEEVKQYLQLDRLREGMFYVAGELFG
                     FTFTPLPEGKVPVFHPDVKVWEVKVKGTGDLVGLWYLDPYARAGKRSGAWASTYRDHE
                     TFDGRRNVLGSNNSNFVKGVPGEPVLISWTDAETLFHEFGHALHYFSSNVAYPTLNGG
                     VRDYTEFQSQLLEHWLLTDRVIDKFLVHVKTGKPMPKALVAKIQKAATFNKGFETTEY
                     LASALMDMKYHTVDPKGIDPKAFEKTTLATLKMPDEIVMRHRSTQFTHVFSGEGYAAG
                     YYGYIWADVLTADAAEAFQAAPGGYYDRALGKKLVDNLFAVRNAVDPADAYRAFRGRD
                     AQIGALMRDRGFPVPAGAPASAK"
     gene            2436..>2813
                     /locus_tag="VFD06_07100"
     CDS             2436..>2813
                     /locus_tag="VFD06_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03340.1"
                     /translation="MKLRIAGLLMLAGLALASASGIVPLAGESPLPIVRGDAWKYLKG
                     TAEPPAAWNTSGFDDSAWLTGASGIGYGDNDDATVLSDMSNTYSTVYMRRLFDVPNPA
                     ALTGLEMRVDYDDGFVAWINGVEV"
BASE COUNT          505 a          951 c          946 g          411 t
ORIGIN      
        1 gcgctggccg gcctcgtggt cgccctcggt gccgtcgcca tcgcggcgga ggagaacgct
       61 gtgaaggaca acgtcctgct ggccgagtgg accggtcctt acggcggcgt gccggcgttc
      121 gaccgggtcc gggtcgccga catgaagccg gccctcgagg cggcgatggc gcagaacctc
      181 caggagctgg aggcgatcgc gaaggacccc gcgccgccga cgttcgagaa caccatcgcg
      241 gccatggaga agaccggccg cacctacgat cgggtgatca cgtattgggg cctctggggc
      301 cagaacctgt ccacgcccga ataccgcgag gtgcaggagg agatggcgcc gcggctggcc
      361 gagttccgct cgaggatcac ccagaacgcc gcgctgttcg cgcgcgtcaa ggcggtgtac
      421 gacgcgagga cgacctccgg tctccggccg gaccagcagc gtctcgtcta tctcatctac
      481 gacagcttcg cccgcgacgg tgcggccctc accggagcgg cgaaagagcg ccacgccgcc
      541 atcaacagcc gcctcgcgga gctctacacc cgcttcgcca acaacgtcct cgccgacgaa
      601 gagtcctacg tgacctggct gacgaaggag cagaccggcg gcctgccgga gtcgctcctg
      661 aagtcggcgg cggccgcggc ggaggagaaa gggcgcaagg gggaatacgc catcaccaac
      721 acccgctcct cggtcgagcc gttcctgacg tactcgaccg accgcgccct gcgcgagaag
      781 gtctggcgcg ccttctacgc ccgcggcggc aatggcgacg agcacgacaa caacgcggtg
      841 atcgccgaga tcctcgcgct ccggaacgag cgtgcgcacc tgctcggcta tccgaactac
      901 gccgcgtggc agaccgagaa caagatggcc aagaccccgg agcgggcgac ggcgctgatg
      961 caggaggtct gggcggccgc caccgcgcgt gtcaaggaag aggtcgccga catgcaagcg
     1021 gtggccgaca aggacaaggc cggcgtcacc atcgccggct gggactatcg cttctacgcc
     1081 gagaaggtgc ggcgcgcccg ctacgacctc gactcggagg aggtgaagca gtacctgcag
     1141 ctcgatcgtc tgcgcgaggg gatgttctac gtcgccggcg agctgttcgg attcacgttc
     1201 acccccctgc cggagggcaa ggtcccggtg ttccacccgg acgtgaaggt ctgggaggtg
     1261 aaggtgaagg ggaccggcga cctcgtcggc ctctggtacc tcgacccgta cgcccgcgcc
     1321 ggcaagcgtt cgggggcgtg ggcgagcacc taccgtgacc acgagacctt cgacggcagg
     1381 cgcaacgtcc tgggctcgaa caactcgaac ttcgtgaagg gagtgccggg ggagccggtg
     1441 ctgatctcct ggaccgacgc cgagacgctg ttccacgagt tcgggcacgc gctgcactac
     1501 ttctcgtcga acgtcgccta tcccactctc aacggcggcg tgcgcgacta cactgagttc
     1561 cagtcgcagc tcctcgagca ctggctcctg accgaccggg tgatcgacaa gttcctcgtg
     1621 cacgtcaaga ccggaaagcc gatgccgaag gcgctggtcg cgaagatcca gaaggcggcc
     1681 accttcaaca aggggttcga gacgacggag tacctcgcgt cggcgctgat ggacatgaag
     1741 taccacaccg tcgatccgaa ggggatcgac ccgaaggcgt tcgagaagac gaccctcgcg
     1801 acgctgaaga tgccggacga gatcgtcatg cggcaccgct cgacgcagtt tacccacgtc
     1861 ttctcgggcg aaggctacgc cgccgggtac tacggctaca tctgggccga cgtgctgacc
     1921 gcggacgccg cggaggcgtt ccaggccgct ccgggcgggt actacgacag ggcgctgggg
     1981 aagaagctgg tggacaacct gttcgcggtg cgcaacgccg tcgatcccgc cgacgcctac
     2041 cgcgccttcc ggggccgcga cgcccagatc ggcgcgctca tgcgcgaccg cggcttcccg
     2101 gtgccggccg gagctcccgc ctcggcgaaa taacgcccgc aggaaaccgc agacagaatc
     2161 ggtgcgcctg cgggcgcttc agcgggcgag gttcgcgcaa gcgagcacgc gctggaagcg
     2221 gggatccagg gagccctgtc ccgggggtcg gccactcccc aaagatgggg aagtttgctg
     2281 catgaccaag ttttcatttg cgatacagca acttgtgcgt agaatcacgc agcctcagcc
     2341 acatgccagt gagtcgcgcc cggtccgccg ccaccgggtt tgtcgcggtc atccgctccg
     2401 gaaccggggg gttcatgtca cgactctgct cgtcgatgaa actgagaatc gcaggtcttc
     2461 tcatgctcgc ggggctcgcc ctcgccagcg ccagcggaat cgtcccgctc gccggcgagt
     2521 cccccctgcc gatcgtccgg ggggatgcct ggaagtacct gaaggggacg gccgagcccc
     2581 ccgccgcctg gaacacctcc ggtttcgacg actccgcctg gctgacaggc gcgagcggca
     2641 tcggctacgg cgacaacgac gacgccaccg tcctctccga catgagcaac acctactcca
     2701 cggtgtacat gcgccggctg ttcgatgtcc cgaaccctgc cgccctcacc ggactcgaga
     2761 tgagggtcga ttacgacgac gggttcgtcg cctggatcaa cggggtggag gtg
//
LOCUS       DASRIQ010000292         6609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_373135, whole genome shotgun sequence.
ACCESSION   DASRIQ010000292 DASRIQ010000000
VERSION     DASRIQ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373135"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VFD06_07105"
     CDS             <1..1540
                     /locus_tag="VFD06_07105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN03341.1"
                     /translation="APEDRRADLAASRRGLERILGTPVRHLAYPNGRSADWDHAVAED
                     ARAAGFESAVTTIEGINRGETDPFRLARISVGDDTTDALALRLLPAVARARARVHARR
                     GGGARPQNVGRPLRVAFIGGRGLGSAYSGIERYYEEIGSRLAAGGMEVIAYCRPHFAP
                     TGTTWRGITVRRVPTIRSKHLETFVHTVLSTADVCLRRVDLVQFHALGSSPFAWVPRL
                     FGKRTIVSVRGLDWQRAKWGALARLYLRFCETTAMSCPDATAVVSRTLRDHFQARFGR
                     PVRYIPNGVARIERAVAAEIGRFGLEPGRYFLYAGRLSPEKGLHVAIEAHRPLDGVQL
                     ALAGGSSYSEGYMRELRAMGGDRVVFTGFLTGPLLQEIYSNALAFLLPSRMEGLSVAL
                     LEAMAYGLPVIASDIPENRELVDECGGYLFRLDDVEDLRRALRSVAADPAAAAETGRR
                     ARERVRALFDWDRIAAETAAFYGEVMAGTGRAPSRSAAPPGVKTTAAPRGAATAPADP
                     ASNG"
     gene            1558..2199
                     /locus_tag="VFD06_07110"
     CDS             1558..2199
                     /locus_tag="VFD06_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZN03342.1"
                     /translation="MSSETPRTKDRTRHEREVEEIVRRGYERHRAALQRLGEGPALER
                     LRRLALLAHEVLARGGKVLACGNGGSASDAQHFAAELVGRYKDERPALAAVALTSDGA
                     VLTCLANDYDYERIFARQVEALGRPGDLLLAISTSGRSPNVLRALETARARGLSTAAL
                     LGRDGGPALPLADVAVVVEDEETARIQEGHALLIHLLCEALDHLPRPGNAPPR"
     gene            2196..3641
                     /locus_tag="VFD06_07115"
     CDS             2196..3641
                     /locus_tag="VFD06_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN03343.1"
                     /translation="MNLPAAGGQAAARGRAAAALLPDLLRRLRVGWWLGGTILVVAGW
                     AIATLTPSQACAVIGATVGLIFWVQSPGLAVYGLAAIAPFSLSFSVGGIDGVGLRDIL
                     LAALAVSALTTLAGGSTRWARFRAPRTKMLLILWAFLLVWGAITFLLGPANRWMLSDK
                     IHNAWFVWCDIGRPLSVFPLVLACLDGRRMMERILLLLVLVGAGVAVNAILLAGTMDD
                     NATGHFETGNALAGYLILILPLAAARLATTKIPWERAVCGVSTLLMLRALWLAGSRGG
                     FVAFLVSMAVLACFIPRRRVAAVAVAGTAVLALVIGMRGGLSSLPMLERFAVLSDPKE
                     VETFQWRQEQWGIFIQRIQDRPMLGYGSDVDESLKDLDRARTAHNAFLALSVKSGIPV
                     AAAWACILILLAALGVRCALQPWENDDRPFWFALLAFLAALLTHNIVESTLLTPIVQD
                     IFWIVTAGAMLLGLPSGRPADAVRFGPSPSA"
     gene            complement(3591..4859)
                     /locus_tag="VFD06_07120"
     CDS             complement(3591..4859)
                     /locus_tag="VFD06_07120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN03344.1"
                     /translation="MSGAPHRRAEALRLLIVAPEYPPATGGMETHAAETAARLARRGH
                     QVTVITAAGGGPSGAGVIPAEPLLDRRFGRALRCLARRARATGADAVLLMNAGYAPLA
                     LRRTDPLPPILVRTVGNDAYGAWHGPRLPLRVLFWRLPHHRADSLGARLRRLDQARRV
                     EAVLAGLGRCDAVLCNSGYTRDRLRSLGVDPARLGLLVGGVDPDLFRPDPSRAPEPDR
                     PILGTSGRLKPIKGFDVALEATARLRAAGLRPRLLVAGSGPDERRLRERASALALRDA
                     VEFLGDQPRQAMPAFYRRLDLYLQPSVEVRHEATGVAQEESMGRAILEAQASGVPVVA
                     SRTGGIPDVVEDGTTGVLVPPKDAARLAEAIAVLVADPERRRQLAATARRVVEERFSW
                     EAVVEATEAAIASALRRRRGAEADRVGGPA"
     gene            complement(4856..5962)
                     /locus_tag="VFD06_07125"
     CDS             complement(4856..5962)
                     /locus_tag="VFD06_07125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN03345.1"
                     /translation="MPKALLVTQDLQRAGAQRQCVELALGLLRAGWKVEVACLEAGGA
                     LAAELQAAGVPIHQMPRRWRWDLSPAFGLAGLVARLDPDVVQTFLFLPNFYGRAARLL
                     RRPRLLVSSLRSTGIEGWPRYLAEVVLAPLCDRILANSEAGRNDLVARGVAPRRVVVV
                     RNGMRLERFDAALAGATTPAGCGARLGMVAQMERRKDHIGLVEALARVRARVPEARLV
                     LAGDGSLRQAVEERVARLGLSDSVELPGIVERPETLYAALAIYVQASAAEEGTSNSIL
                     EAMACGRPVIATDIGGNREVVEDGTTGLVVPPRDPDALARAILSLLADPGRLEAMGEA
                     GQRAVRTRFSLDTMIRETLAAYRGVPGRAPDERR"
     gene            complement(5970..>6609)
                     /locus_tag="VFD06_07130"
     CDS             complement(5970..>6609)
                     /locus_tag="VFD06_07130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN03346.1"
                     /translation="LERFHPGVRPDSGVRFPAGVAPIVGFVGSFRRWHGTDLMTRMAR
                     EVGAARPDVGFLFVGDGPEAAAVQEAIRPFGARAVMTGRVPHARVPGLTAAFDIGVLP
                     ETLFYASPLKVIEWMAAGRSVVAPGYPALDDIVENGKEALLFPPGDADALVRAVLRLI
                     DDGALRARLAAAAAERAAKSLSWVENARRVLRACETARASRLPGGDTSHRPA"
BASE COUNT          835 a         2475 c         2408 g          891 t
ORIGIN      
        1 cgccccggag gatcgccgcg ccgatctcgc cgcttcgcgg cgaggcctcg agcggatcct
       61 cgggacgccg gtccgccacc tcgcctaccc gaacggccgg tcggccgact gggaccatgc
      121 ggtggcggag gacgcccgtg cggccggctt cgagtcggcg gtgacgacga tcgaggggat
      181 caaccggggc gagacggatc cgttccgcct cgcccgcatc agcgtcggcg acgacacgac
      241 cgacgcgctg gcgctccggc tgctgccggc cgtggcgcgg gctcgcgccc gggtccacgc
      301 gcggcggggc ggcggcgcgc gtccgcagaa cgtcggccga ccgctccgcg tggccttcat
      361 cggcgggcgc gggctcggct ccgcctacag cggcatcgag cgctactacg aggagatcgg
      421 cagccgcctc gcggccggcg ggatggaagt catcgcctac tgtcgcccgc acttcgctcc
      481 gaccgggacg acctggagag gcatcacggt gcgacgcgtg ccgaccatcc gcagcaagca
      541 cctcgagacc ttcgtgcaca cggtcctctc gaccgccgac gtctgcctgc ggcgcgtcga
      601 cctggtgcaa ttccacgcgc tcggctcgtc gccgttcgcc tgggtgccgc gcctgttcgg
      661 caagcgcacc atcgtctcgg tccgcgggct cgactggcag cgcgccaagt ggggggcact
      721 cgcgcgcctc tacctgcgct tctgcgagac caccgcgatg tcgtgcccgg acgccaccgc
      781 ggtcgtctcc cgcacgctgc gcgatcactt ccaggcacgc ttcggccgtc cggtgcgcta
      841 cattcccaac ggcgtcgcgc ggatcgagcg ggcggtcgcc gcggagatcg gccgcttcgg
      901 cctcgagccg gggcgctact tcctgtacgc ggggaggctc tcgcccgaga aggggctgca
      961 cgtggccatc gaggcgcatc gtccgctcga cggcgtccag ctggccctcg ccggtggctc
     1021 cagttattcc gagggctaca tgcgggagct tcgagcgatg ggaggggacc gggtggtatt
     1081 caccgggttc ctcaccgggc cgttgctcca ggagatctac tcgaacgcgc tcgcgttcct
     1141 gctcccgtcg cgcatggagg gactgtcggt ggcgctcctc gaggcgatgg cgtatgggct
     1201 tccggtcatc gccagcgaca tcccggagaa ccgcgagctg gtggacgagt gcggcggcta
     1261 cctgttccgt ctcgacgacg tggaggacct gcggcgggcg ctccggtcgg tcgcggcgga
     1321 cccggccgcc gccgcggaga ccgggcgacg ggcgcgcgag cgggtccggg cgctcttcga
     1381 ctgggaccgc atcgctgccg agaccgccgc gttctacggc gaggtgatgg ccggaacggg
     1441 gagggcgccc tccaggtccg ccgcccctcc cggcgtgaag acgacggccg ccccgcgggg
     1501 ggcggcgacc gccccggccg atccggcctc caacggctga ccttcgagga gcgagccatg
     1561 tcctccgaga cgccacgaac aaaagacagg acgcgccacg agcgggaggt cgaggagatc
     1621 gtgcgccggg gatacgagcg ccaccgggcc gcactgcagc ggctcgggga agggccggcg
     1681 ctcgagcggc tgcggcggct cgcgctgctg gcgcacgagg tcctggcgcg cgggggcaag
     1741 gtgctggcgt gcggcaacgg gggaagcgcg tccgacgcac agcacttcgc cgccgagctc
     1801 gtcggacgct acaaagacga gcgcccggcg ctcgccgccg tggccctcac cagcgacggg
     1861 gcggtgctga cctgtctcgc caacgactac gactacgagc ggatcttcgc ccggcaggtc
     1921 gaggcgctgg ggcggcccgg cgatctgctc ctcgcgatca gcacgtcggg gcgatcgccg
     1981 aacgtgctgc gggcgctcga gacggcccgg gcccggggac tctcgacggc ggcgctcctg
     2041 ggccgggacg gcgggccggc gctgccgctc gccgacgtcg cggtcgtggt ggaggacgag
     2101 gagaccgccc gcatccagga ggggcacgcc ctgctcatcc acctcctgtg cgaagccctg
     2161 gatcacctgc ctcgtccggg gaacgcgccg ccgcgatgaa cctccccgcc gccggtggcc
     2221 aggcggcggc gcgcggccgc gccgcggcgg cgctcctccc ggacctgctg cgccgcctgc
     2281 gcgtcggctg gtggctcggg gggacgatcc tcgtcgtcgc cggctgggcg atcgccaccc
     2341 tgacgccttc gcaggcctgc gcggtgatcg gcgcgacggt cggactcatc ttctgggtgc
     2401 agtcgccggg gctcgccgtg tacggactcg ccgcgatcgc ccccttcagc ctgagcttct
     2461 cggtcggcgg gatcgacggc gtcggcctgc gcgacatcct cctggcggcc ctcgcggtct
     2521 cggcgctgac gacgctcgcc ggcggatcga cgcggtgggc gcgcttccgc gcgcctcgga
     2581 cgaaaatgct gctcatcctc tgggcgtttc tcctggtctg gggcgcgatc accttcctgc
     2641 tcggaccggc gaatcgctgg atgctcagcg acaagatcca caacgcctgg ttcgtctggt
     2701 gcgacatcgg ccgcccgctc tcggtctttc ccctggtcct ggcctgcctg gacggtcgcc
     2761 ggatgatgga gaggatcctg ctgctcctgg tgctcgtcgg ggcgggcgtc gcggtcaacg
     2821 ccatcctgct cgccggcacc atggacgaca acgccaccgg ccacttcgag accggcaacg
     2881 cgctggccgg gtatctgatc ctgatcctgc cgctggcggc ggcgcggctg gcgacgacga
     2941 agattccgtg ggagcgcgcc gtgtgcgggg tgtccacgct cctcatgctc cgggcgctgt
     3001 ggctcgcggg gtcgcgcggc ggcttcgtcg cctttctcgt gtccatggcg gtgctcgcct
     3061 gtttcatccc gcgccgccgc gtcgccgccg tcgccgtcgc cggcaccgcg gtgctcgccc
     3121 tggtcatcgg catgcgcggc gggctgtcga gcctgccgat gctggagcgc ttcgccgtcc
     3181 tgagcgatcc caaggaggtc gagacgtttc agtggcgtca ggagcagtgg ggaatcttca
     3241 tccagcggat ccaggatcgg ccgatgctcg ggtacggctc cgacgtggac gagtccctga
     3301 aggacctgga ccgcgcccgc acggcgcaca acgcctttct cgccctgtcg gtcaagtcgg
     3361 ggattccggt ggcggccgcc tgggcgtgca tcttgatcct gctcgccgcc ctcggggtgc
     3421 gctgcgcgct ccagccgtgg gagaacgacg atcgtccttt ctggttcgcg ctcctggcct
     3481 tcctcgccgc gctcctgacg cacaacatcg tggagtccac gctcctgacg ccgatcgtgc
     3541 aggacatctt ctggatcgtc accgccggag ccatgctgct cggcctgccg tcaggccggc
     3601 ccgccgacgc ggtccgcttc ggcccctcgc cgtcggcgta acgccgaggc gatcgccgcc
     3661 tcggtcgcct ccaccaccgc ctcccaggag aagcgctcct cgacgacgcg ccgcgccgtc
     3721 gcggccagct gccgtcgccg ctccgggtcg gcgacgagga ctgcgatcgc ctccgccagc
     3781 cgcgcggcgt ccttcggagg cacgaggacg ccggtcgtgc cgtcctcgac gacgtccggg
     3841 atcccgccgg tgcggctggc cacgaccggc acgccgctcg cctgcgcctc gaggatggcg
     3901 cgccccatcg actcctcctg cgccacgccc gtggcctcgt gccgcacctc caccgacggc
     3961 tggaggtaca ggtcgaggcg gcggtagaac gccggcatcg cctgccgggg ctgatctccg
     4021 aggaactcca ccgcgtcgcg cagtgcgagc gccgaagcgc gctcgcggag ccgccgctcg
     4081 tccggccccg agccggcgac gagcaggcgc ggccgcagcc ctgcggcgcg caggcgggcg
     4141 gtcgcctcga gcgccacgtc gaaccccttg atcggcttga gtcgcccgct cgtcccgagg
     4201 atcggccggt ccggctccgg cgcgcgggac ggatccggcc ggaagagatc ggggtcgact
     4261 cctccgacga gaagcccgag gcgcgccggg tcgaccccga ggcttcgcag ccgatcccgc
     4321 gtgtagccgc tgttgcagag gaccgcgtcg cagcgcccga ggccggcgag caccgcctcg
     4381 acgcgccgcg cctgatcgag gcgccgcaac cgggcgccga gagagtccgc ccggtggtgc
     4441 ggcaggcgcc agaagagaac gcgcagcggc agccgcggtc cgtgccaggc gccgtacgcg
     4501 tcgttgccga cggtgcggac caggatcggc ggcagcggat ccgtccgtcg aagggcgagc
     4561 ggggcgtagc cggcgttcat gaggagcacg gcgtcggcgc cggtcgcgcg cgcgcggcgg
     4621 gcgaggcacc gaagggcccg gccgaaacgg cgatcgagga gtggctccgc ggggatcacg
     4681 cccgccccgg aggggccgcc cccggccgcc gtgatcaccg tgacctggtg cccgcgccgc
     4741 gccagccgcg cggcggtctc ggccgcgtgc gtctccatcc caccggtggc cggcggatac
     4801 tcgggggcga cgatgaggag gcgcagggcc tcggcccggc ggtgcggcgc tccgctcatc
     4861 gccgttcgtc cggagcgcgc ccgggcacgc cgcgatacgc cgcgagcgtt tcgcggatca
     4921 tcgtgtccag ggagaagcgc gtccgcaccg cgcgctgccc ggcctctccc atggcctcga
     4981 ggcgcccggg gtcggcgagc agcgagagga tcgcccgcgc cagcgcgtcc ggatcgcgcg
     5041 gcggcacgac gagaccggtc gtcccgtcct ccaccacttc gcggttccct ccgatgtcgg
     5101 tggcgatgac cggccggcca caggccatcg cctccaggat gctgttgctc gtcccttcct
     5161 cggccgccga cgcctgcacg tagatcgcca gcgcggcata cagggtctcc ggccgctcca
     5221 cgattccggg gagctccacc gaatccgaga gtccgagccg cgcgacccgc tcctcgaccg
     5281 cctggcggag cgagccgtcc cccgccagga cgagccgcgc ctccgggacg cgggcgcgca
     5341 cccgcgccaa cgcctcgacc agcccgatgt ggtctttgcg ccgctccatc tgggccacca
     5401 ttcccaggcg cgcgccgcat ccggcgggtg tcgtcgcgcc ggcgagggcg gcgtcgaagc
     5461 gctccaagcg catcccgttg cgcaccacga cgacgcgtcg gggggcgacg ccgcgcgcca
     5521 cgaggtcgtt tcggcccgcc tcggagttgg cgaggatccg gtcgcacagg ggcgcgagca
     5581 ccacctcggc gagataccgc ggccagcctt cgatcccggt cgaacgcagc gaagacacca
     5641 ggagccgcgg ccggcgcagc agacgtgccg cccgcccgta gaagttgggg aggaacagga
     5701 aggtctgcac gacgtccgga tcgaggcgcg cgaccaggcc ggcgagaccg aaggcggggg
     5761 agagatccca gcgccagcgc cgcggcatct ggtgaatcgg aactccggcc gcctgaagct
     5821 ccgccgcgag cgcgcccccg gcttcgaggc aggccacttc gaccttccag ccggcgcgca
     5881 ggaggccgag ggccagctcg acgcactgcc gctgcgcgcc ggcgcgctgc agatcctgcg
     5941 tgaccaggag cgccttcggc atgatccgct caggccgggc ggtgcgacgt gtcgccgccg
     6001 gggagccggg acgcgcgcgc cgtctcgcag gcccgcagga cccttcgggc gttctcgacc
     6061 caggagaggc tcttcgccgc gcgctccgcc gccgccgcgg ccagccgggc gcgcaaggcg
     6121 ccgtcgtcga tcagccgcag caccgcccgc acgagcgcgt ccgcgtcgcc cggcgggaag
     6181 agaagcgctt ccttgccgtt ctccacgatg tcgtcgaggg ccgggtagcc gggggcgacg
     6241 acactccgtc cggcggccat ccattcgatc accttgagcg gcgaggcgta gaagagggtc
     6301 tcgggaagga cgccgatgtc gaaggccgcg gtcaggcccg gcacgcgcgc gtgcgggacc
     6361 cgtccggtca tcaccgcgcg cgccccgaac ggccggatcg cctcctgcac cgccgcggcc
     6421 tccggtccgt cccccacgaa gaggaatccg acgtcgggcc gcgccgcgcc gacctcgcgc
     6481 gccatccggg tcatgagatc ggtcccgtgc cagcggcgga acgatccgac gaagccgacg
     6541 atcggcgcga ccccggccgg gaaccgcact ccagagtcgg ggcgcacgcc gggatggaac
     6601 cgctccaga
//
LOCUS       DASRIQ010000293         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3789050, whole genome shotgun sequence.
ACCESSION   DASRIQ010000293 DASRIQ010000000
VERSION     DASRIQ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3789050"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2802
                     /locus_tag="VFD06_07135"
     CDS             <1..2802
                     /locus_tag="VFD06_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN03347.1"
                     /translation="DVRRGRVPSLLYGGPSTPVPGSAAPLRALGLSSVLLLGLGSGGR
                     RTGALLLASREADAFAGEPMIMAELLASEMSEHCERVRASNGPGGRGAQGADDAEMKR
                     LRARNAELEAQAAIVSAASLSHDADRQIELTLRKALDLTGHRGGAIYLVETNDAGDDI
                     LCLARGLGDPDWLEQARLPRWRKGEGPLGKVWEKKEGLALGDLKEDPDGFARAALERA
                     GYHRVCAEPLTAGARILGVLQMFGEGAHPYDAAERSLVRAIARQVGSAIHNARLLGDL
                     TRHSLELEWEVDRLRGGEARRASEGRGIADLLAAGAQAQDGESRASAVLARLLESIRA
                     DAAAILLLERGGLALRLMAQRGFPDAAANALWSLDADDAVIVRGIEAGDGGAVLDLAD
                     ERLLEGIWPRRAGYRHVLALPCRTGGELRGILLVATRYRDFFGEPERCEVRPFADLLS
                     LLLDAAHHAIPPQAAPRPDDEPALHPMAAAPVAAAPDRPASGRGDDPTTADDATGRDD
                     DAAPRSEQLIEAQRMESIGRLASGIAHDFNNSLGAILGHATHIKTLVPDHNPVHGKAV
                     VIEEQTQRAADLMRRLVTFSGGPSGKRELIDINALVEETVAMLLRALDPSVVLETRSS
                     PGLPPVEADAGQIRQALLNLAVNARDAMPDGGRIVFETRTGHLDRRETGPGAPIAAGD
                     YVSIAVNDNGAGMAKDVADQVFEPFFTTKPVSHGSGLGLTVVQDIVQELGGHVSLSSA
                     PGIGTSVRLYLPAAARPDLPRDAGARGAEATAVMATTGPMPVIDQTEAEPPALPGTTS
                     NPALETRPLHSPTLPARILVVDDEPVLREMTVEMLRSRGHEVLMARDGVEALDIYRQE
                     WGRIDLVVLDMIMPRLGGIETFRRLIGMDRKARILLCSGLSHNQQGQEAVRQGALGLL
                     PKPFGMNELFGWVDKALKR"
     gene            2873..>3207
                     /locus_tag="VFD06_07140"
     CDS             2873..>3207
                     /locus_tag="VFD06_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03348.1"
                     /translation="MAGRGARIASVLLSASLLGAGSGKPAPASAGPATTDSTAASPGR
                     MALERGIALRKDRPADAAPILQEALRLLQSEGNLGGQAEARRNLGILERQAGRDDAAL
                     AHYQAALDLA"
BASE COUNT          431 a         1170 c         1203 g          403 t
ORIGIN      
        1 gacgtgcggc gcgggcgcgt cccgagcctg ctctacggcg gcccttcgac ccccgtcccg
       61 gggtcggcgg cgccgttgcg ggcgctcgga ctgtcgtcgg tgctgctcct cgggctcggc
      121 agcggcggcc ggcgcaccgg ggcgctcctc ctcgccagcc gcgaggcgga cgcgttcgcc
      181 ggcgagccga tgattatggc ggagctcctg gcgtcggaga tgtcggagca ctgcgagcgg
      241 gtgcgcgcct ccaacggtcc cggcggccgg ggcgcgcagg gggcggatga cgccgagatg
      301 aagcgcctgc gggcgcgcaa cgccgaactc gaagcgcagg cggcgatcgt ctccgccgcc
      361 agcctcagcc acgacgccga tcggcagatc gagctgacct tgcgcaaggc gctcgatctg
      421 accggccacc gcggcggggc catctacctg gtggagacca acgacgcggg ggacgacatc
      481 ctgtgcctgg cgcgcggtct cggcgatccg gactggctgg agcaggcccg gctgccgcgc
      541 tggcgcaagg gagaagggcc cctcggaaaa gtctgggaga agaaggaagg gctcgccctc
      601 ggcgacctga aagaggatcc ggacggcttc gcgcgcgcgg cgctcgaacg ggccggctac
      661 catcgcgtct gcgccgagcc gctcaccgcc ggagcgcgca tcctcggcgt gctgcagatg
      721 ttcggcgagg gggcgcaccc ctacgacgcc gccgagcgga gcctcgtgcg ggcgatcgcc
      781 cgccaggtcg gcagcgccat ccacaacgcg cggctgctcg gggacctgac gcgccacagc
      841 ctggagctgg agtgggaggt ggaccggctg cgcggcggtg aagcgcgccg cgcgtcggag
      901 ggacggggga tcgccgatct cctcgccgcc ggggcgcagg cccaggacgg cgagtcgcgc
      961 gcctccgccg tcctggcgcg cctcctcgag tcgatccggg ccgacgcggc cgccatcctc
     1021 ctgctcgagc ggggggggct cgcgctgcgc ctgatggcgc agagggggtt cccggacgcc
     1081 gccgccaacg cgctgtggag cctcgacgcc gacgacgcgg tgatcgtgcg cgggatcgag
     1141 gcgggggacg ggggggcggt gctcgacctg gccgacgagc gcctgctcga aggcatctgg
     1201 ccgcgccgcg ccgggtaccg gcacgtcctc gcgcttccgt gccgcaccgg cggagagctg
     1261 cgcgggatcc tgctcgtggc gacgcgctac cgcgatttct tcggggagcc ggaacgctgc
     1321 gaggtgcggc cgttcgccga cctgctgtcg ctcctgctcg acgccgccca ccatgccatt
     1381 ccgccgcagg ccgcgccgcg tcccgacgac gagccggccc tgcatccgat ggccgccgcc
     1441 ccggtcgcgg cggcgccgga ccggccggcc tccggccggg gggacgatcc gacgacggcc
     1501 gacgacgcca ccgggcgcga cgacgacgcg gccccccgct ccgagcagct catcgaagcg
     1561 cagcggatgg agtcgatcgg gcggctcgcc tccggcatcg cccacgactt caacaactcg
     1621 ctcggcgcca tcctgggcca cgccacccac atcaagacgc tcgtccccga ccacaacccg
     1681 gtgcacggca aggcggtcgt catcgaggag cagacgcagc gcgccgccga tctcatgcgc
     1741 cggctggtga ccttctcggg agggccgagc gggaagcgcg agctcatcga catcaacgcg
     1801 ctcgtggagg agacggtggc gatgctcctg cgcgcgctcg atccgtcggt cgtcctcgag
     1861 acgcgctcct cccccgggct gccgccggtc gaagcggacg cggggcagat ccgccaggcg
     1921 ctcctgaatc tggcggtgaa cgcgcgcgat gcgatgccgg acggcgggcg gatcgtcttc
     1981 gagacgcgca ccgggcacct cgaccggcgc gagaccggcc cgggcgcgcc gatcgcggcg
     2041 ggggactacg tctcgatcgc ggtcaacgac aacggcgccg gcatggccaa ggatgtcgcc
     2101 gaccaggtgt tcgagccgtt cttcaccacc aagccggtgt cgcacgggag cggcctcggt
     2161 ctgacggtcg tccaggacat cgtgcaggaa ctcggcggtc acgtgtccct gtcgagcgcc
     2221 cccggtatcg ggacctcggt gcgcctgtac ctcccggccg cggcgcgtcc ggatctgccg
     2281 cgcgacgccg gagcgcgggg cgccgaggcg accgccgtga tggcgaccac cgggccgatg
     2341 ccggtgatcg accagacgga ggcggagccg ccggctctcc ccggcacgac gtcgaacccg
     2401 gcgctcgaga cgcggccgct ccattcgccg acgctgccgg cgcggatcct cgtcgtggac
     2461 gacgagccgg tgctgcgcga gatgacggtc gagatgctca ggagccgcgg ccacgaggtg
     2521 ctgatggccc gcgacggcgt cgaggccctc gacatctacc gccaggagtg ggggcggatc
     2581 gatctcgtgg tgctcgacat gatcatgccg cgcctcggcg gcatcgagac cttccggcgt
     2641 ctcatcggca tggaccggaa ggcccgcatc cttctctgct cggggttgag ccacaaccag
     2701 cagggccagg aggcggtgcg ccagggggcg ctggggctcc tcccgaagcc gttcgggatg
     2761 aacgagctct tcggctgggt ggacaaggcg ctcaagcgct gacggcggcc gcgggacggc
     2821 gccggcgaaa tcctccgcac gatcggggtg ctatactcgc ccccttcctc acatggccgg
     2881 tcgcggggct cgcatcgcgt cggtgctcct gtcggcgtcg cttctgggcg ccgggtcggg
     2941 aaagcctgcg ccggcgtccg ccggccctgc caccaccgat tcgaccgccg cctcgccggg
     3001 ccgcatggcg ctcgaacggg ggatcgccct gagaaaggat cgccccgccg acgcggcgcc
     3061 gatcctccag gaggcgctcc ggctgctgca gtccgaaggg aacctggggg ggcaggcgga
     3121 agcccgtcgc aacctcggca tcctcgagcg ccaggccggc cgggacgacg cggccctggc
     3181 gcactaccag gcggcgctcg acctcgc
//
LOCUS       DASRIQ010000294         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1175426, whole genome shotgun sequence.
ACCESSION   DASRIQ010000294 DASRIQ010000000
VERSION     DASRIQ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175426"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFD06_07145"
     CDS             complement(<1..363)
                     /locus_tag="VFD06_07145"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HZN03349.1"
                     /translation="MTTKIALRWTEGAIVEPWTRANLAAAAGLTRLLGMEATVIGTNL
                     SSGGATLNVQNGCDGIHAALILVAAIAAFPAPLSRRLVGIAAGTLAIFGLNAVRLVNL
                     LVVAVHLPGWLDFFHIYVW"
     gene            508..>2843
                     /gene="uvrA"
                     /locus_tag="VFD06_07150"
     CDS             508..>2843
                     /gene="uvrA"
                     /locus_tag="VFD06_07150"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZN03350.1"
                     /translation="MAADSIVIKGAAEHNLKHIDLTLPRNRMIVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLEQMEKPEVESIEGLSPAISIEQKTTSKNPRSTVGT
                     VTEIYDYLRLLFARIGLPHCPQCGRPIARQTVQQIVDQVMEIPAGARLMVLAPIVRGR
                     KGEYKKELREMLRKGFVRARIDDEVMEIDEEIDLDKKKAHTIEIVVDRLVLKPEVKQR
                     LTDSLETALKLAEGLATVNVLGEQGRDIIFSDSLACPYDGVSVPELAPRMFSFNSPYG
                     ACPTCDGLGSHQEIDPAKLVANPTQSVADGAISGTWGFGKSMLRMAVEAMGRAMKFDP
                     NLPFKNLPKEVQRVLLYGSGRTEFDYEYKEGRNRYFFRKPFTGVIPSMMKRYRESDSS
                     ALHEEIERYMSLEPCAVCEGARLKPESLAVTVADHNIHYYTGLTIKEANATFNDMTLS
                     ARDQQIAHMVMKEIRERLGFLLNVGLGYLSLSRTAATLSGGEGQRIRLATQIGSRLTG
                     VLYVLDEPSIGLHQRDNRRLLDTLCAMRDLGNTVIVVEHDEETIRTADHVVDLGPGAG
                     EMGGRVVAQGTPEEIAAHPDSITGQYLSGRRAIPIPKKRRPAAANAKRPEEGWLSVVE
                     AEENNLKKITARFPLALLTCVTGVSGSGKSTLVNETLYKALAKRIYRLPIRPGKHKEI
                     RGIDQLDKVIDIDQSPIGRTPRSNPATYTGLFTPLRELFAQVPEARARGYRVGRFSFN
                     VKGGRCEKCEGDGIIKIEMHFLPDVYVTCEDCKGRRYNR"
BASE COUNT          527 a         1001 c          914 g          401 t
ORIGIN      
        1 ccacacgtag atgtggaaga aatccagcca gccgggcaga tggacggcga cgaccaggag
       61 gttcacgagg cggacggcgt tcaatccgaa gatggccagg gtccccgccg cgatcccgac
      121 gagccgccgg gagagaggcg ccgggaaagc ggcaatcgcc gcgaccagga tgagcgcggc
      181 gtgaatgccg tcgcacccat tctggacatt cagagtcgcg cctcccgagg acaggttcgt
      241 tccgatgacg gtcgcctcca tgcccaggag gcgcgtcaac cccgccgccg cggccaggtt
      301 cgctcgcgtc cagggttcga cgatcgcccc ttccgtccag cgcagggcga tcttggttgt
      361 caagtggagc ctatgaagcc gcagggagca gagttcaggc ttggcggggc cgtggtctga
      421 cccggccggt tgacccgccc gccgccacaa ggtccgcccc tccgagccgg gggggcttgc
      481 caggcccggt ataatctgcc ccatcccatg gccgccgact ccatcgtcat caagggtgcc
      541 gccgagcaca acctcaagca catcgacctg accctgcccc ggaacaggat gatcgtcatc
      601 accgggctgt cggggtcggg gaagtcgtcg ctcgccttcg acaccatcta cgccgagggg
      661 cagcggcgct acgtcgagtc gctctccgcc tacgcccgcc agttcctcga gcagatggag
      721 aagccggagg tcgagtcgat cgaggggctc tcccccgcca tctccatcga gcagaagacg
      781 acttcgaaga acccgcgctc gacggtcggc acggtcaccg agatctacga ctacctgcgc
      841 ctactgttcg cccgcatcgg gctgccgcac tgcccccagt gcgggcggcc gatcgcccgg
      901 cagacggtgc agcagatcgt cgaccaggtg atggaaatcc cggccggcgc gcgcctcatg
      961 gtcctggcgc cgatcgtccg cggccgcaag ggggaataca agaaagagct gcgcgagatg
     1021 ctgcgcaagg ggttcgtgcg cgcccgcatc gacgacgagg tgatggagat cgacgaagag
     1081 atcgacctcg acaagaagaa ggcccacacc atcgagatcg tcgtcgaccg cctggtgctg
     1141 aagccggagg tcaagcagcg cctgaccgat tcgctcgaga ccgccctgaa gctggcggag
     1201 gggctcgcga cggtcaatgt gctgggcgag caggggcgcg acatcatctt cagcgactcg
     1261 ctcgcctgcc cgtacgacgg cgtctcggtc cccgagctgg cgccgcgcat gttctccttc
     1321 aactcgccgt acggcgcctg ccccacctgc gacggcctcg ggtcgcacca ggagatcgac
     1381 ccggcgaagc tcgtcgcgaa cccgacccag tcggtcgccg acggggcgat ctccggcacc
     1441 tgggggttcg gcaagtcgat gctgcggatg gcggtcgagg cgatggggcg ggcgatgaag
     1501 ttcgacccga acctgccgtt caagaacctc cccaaagaag tgcagcgggt cctcctctac
     1561 ggcagcggcc gcaccgagtt cgactacgaa tacaaggaag ggcgcaaccg ctacttcttc
     1621 cgcaagccgt tcaccggcgt catcccgtcg atgatgaagc gctaccgcga gtcggactcc
     1681 tcggcgctgc acgaggagat cgagcgctac atgtcgctcg agccgtgcgc cgtctgcgag
     1741 ggggcccgcc tcaagcccga gtcgctggcg gtcaccgtcg ccgaccacaa catccactac
     1801 tacaccggcc tcaccatcaa ggaggcgaac gccaccttca acgacatgac cctgtcggcg
     1861 cgcgaccagc agatcgccca catggtgatg aaggagatcc gcgagcgcct cggcttcctg
     1921 ctgaacgtcg gcctcggcta cctcagcctg tcccgcaccg ccgcgaccct ctcaggagga
     1981 gaagggcagc ggatccgcct cgcgacgcag atcggctcgc gcctgaccgg cgtgctgtac
     2041 gtcctggacg agccgtcgat cggcctgcac cagcgggaca accggcgcct cctcgacacc
     2101 ctgtgcgcca tgcgcgacct cggcaacacc gtcatcgtcg tcgagcacga cgaggagacg
     2161 atccgcaccg ccgaccacgt ggtggacctc ggccctggcg cgggtgagat ggggggccgc
     2221 gtcgtggcgc aggggacgcc tgaggagatc gccgcccatc ccgactcgat caccggccag
     2281 tacctgagcg gccggcgcgc catcccgatc ccgaagaagc gccgccccgc cgccgccaac
     2341 gccaagcgcc ccgaagaagg ctggctgagc gtcgtcgaag cggaggagaa caatctcaag
     2401 aagatcaccg cccgcttccc gctggcactc ctgacctgcg tcaccggcgt cagcggctcg
     2461 gggaagagca ccctggtcaa cgagacgttg tacaaggcgc tcgccaagcg gatctatagg
     2521 ttgcccatcc gtcccgggaa gcacaaggag atccgcggca tcgaccagct cgacaaagtg
     2581 atcgacatcg accagtcccc catcggccgc accccgcgca gcaacccggc gacctacacc
     2641 ggcctgttca caccgttgcg ggagttgttc gcccaggtgc cggaggcgcg cgcccgcggc
     2701 taccgcgtgg gccgcttctc gttcaacgtc aaggggggcc gctgcgagaa gtgcgaaggg
     2761 gacggcatca tcaagatcga gatgcacttc ctccccgacg tctacgtcac ctgcgaagac
     2821 tgcaaaggcc gccgctacaa ccg
//
LOCUS       DASRIQ010000295         9120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1175727, whole genome shotgun sequence.
ACCESSION   DASRIQ010000295 DASRIQ010000000
VERSION     DASRIQ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175727"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFD06_07155"
     CDS             complement(<1..396)
                     /locus_tag="VFD06_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HZN03351.1"
                     /translation="MTREIGVLGYGAYVPEGRVSAKVLASAWGRNGEGRLPVKEKSVP
                     GLDEDTITMAIEAARHALARAGDAVTPERLGAVFVGTESKPYAVKPSGTVVAEAIGAV
                     PWTSAADFEFACKAGTEALRAAAAFVGAGL"
     gene            complement(393..1055)
                     /locus_tag="VFD06_07160"
     CDS             complement(393..1055)
                     /locus_tag="VFD06_07160"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN03352.1"
                     /translation="MSRAARLDHATRQGQILEKAARVFCERGYEKTSMGDVAAAVGMT
                     KAGVYHHIAGKEDLLAAIMHYGMDLFEERVLSRTEPIADPLARLCATVQGHLLLVLRD
                     RPKEVTVILHEGGALPASARARLDARKKRYIRFLEKTIRELVMRKVARPVDPTVAAYA
                     LLGMVNWTYQWFRPGGRLDEEGLARQMTDFYLRGLLRDDAAAAEVGTGRRRGRRPARR
                     IA"
     gene            1216..2634
                     /locus_tag="VFD06_07165"
     CDS             1216..2634
                     /locus_tag="VFD06_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN03353.1"
                     /translation="MEPAGAAAPEAAREEGLRKSLSFRDVFLFLVTAGVNLQWVATAA
                     ASGPSVMVVWFIGLAAMTVPLSWCTIRLAAHHPDEGGLYVWSRKAFGEAGGFLCGWIY
                     WMTNLPYFPGVLYFAAGNGLFLAGANYRHLSSDRGYFLVFSLLALGLATWMNLVGLSV
                     AKRLNNLGAHARWVGGLLLIGLGATAWWHFGSMTPFDRASLTPRLDLQGLVFWSTIAF
                     ALTGLESVSFMGGEIHDARKVVPRAIAAAVPLIFVIYYLGTAAVLVALPPGEVSGLQG
                     VIEAVAAIESRLSLHGVTRVMAGILLLHSLGSVGAWLGAVARIPYVAGIDRFLPQGFA
                     RLHPRWGTPYVALLTQAVVTVVFIVIGQAGTSVAGAYEVLVGMMVVIYMLPFLFLYGA
                     ALRLPLDRTVAARIATRCLALLGLFTVTAAIGLAMVPAAHEPNKPLAILKIAGSTAAM
                     VGSGVLVYLFRRPRLRSPGAAA"
     gene            complement(2594..4102)
                     /locus_tag="VFD06_07170"
     CDS             complement(2594..4102)
                     /locus_tag="VFD06_07170"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZN03354.1"
                     /translation="MRGFRNPLKPLDLLSPESEEQVHRASMRLLQEQGMVFEEPRAVD
                     LFRRAGQKIDDGAQRVFFDPDFVIETAAKAPAQFVLHARDPDWNVTVGDDTVIFAPVS
                     GPPFVADADRGRRDGTLPDQNDLVRLAETLDVLHHGTPEVACKDLSVESRHLDILHHQ
                     IRLSRKGMIGDAWSTVRARDHIALMGILFRGLDRKAGIGSGGSGGELRDAIRDRAVLV
                     GIINSNSPLRYDTPMLEGLLEYAEAGQVNIITPFIMAGATSPVTLAAAVAQQNAEALG
                     AVVLTQLVRPGAPVMYGSFLTGLEMRTGAPGFGRAEAALGILSSAQMARRYRLPCRGG
                     GILTNSKVPDHQAGQEKMNMLWPLLLGRVHYVLHAAGWLDGGLIASFESLVLDAEVLE
                     MTQRFFGGLLVDDDRLALDVIAHVPVGGHFLGEAHTRRHFKSEFYFPRLADTEAWDSW
                     VKKGSKDAAARATPRWKTLLESYKEPVLDPGIDEELSDYVTRRRRAIEAEGA"
     gene            4299..5594
                     /locus_tag="VFD06_07175"
     CDS             4299..5594
                     /locus_tag="VFD06_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03355.1"
                     /translation="MPRSGHVRRPHRSAPRALLALAILVTSALSGSPLRAGAPDQDEP
                     RLVRAEPGGFVVAPQAGDVALDLYGENLWDADRLWTERNVRVYARHASGDEAWTRLFT
                     GSGDAGNGAPEDASDALARGRVVESLPDRYRFLLPASLWLDRKGTLEFKVVKGTWRRA
                     GEGRWRYQETAASNILKLPVAEAPHRAPEVREISPALLPARHDGGPPPTLWVRASDLT
                     LDPEVRIGGVPCPTVRIDVSLDFIECRVPGDILDHPDTYQVTVRTPVGEAAEGLSLSV
                     VSPPAIAALSPEALPAKGPDALVVVRFSGSEPDTARMRRIDRGGWTTVALRHLEPGAA
                     RLELPAALRGFAGEIEVELGNLAGTARASLALCAEEGPNPSVCSRAGTLAGPGPEVAA
                     AALRRPSGSARPAGIEPASPAPAQGTSSMAVIPLARSAR"
     gene            complement(5543..8545)
                     /locus_tag="VFD06_07180"
     CDS             complement(5543..8545)
                     /locus_tag="VFD06_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN03356.1"
                     /translation="MRRTGPVWVVAGYHVIVPALLMMAGPAPAVPNPPNPPDSASATF
                     RGLGDLPGGVFHSQAYGMSADGRVVVGQSSGNTGREACRWVLPGGPQSLGPVTVGPVG
                     AVADSVSDDGSVIVGSRQLAQTVFLAYRWSEATGLIPLGDLPGGPENSIATDTTSDGS
                     VIVGQSFGPFGIEAYRWTLPTGMQPLGDLPGGVFASNALGISGDGSVIIGHGNSAAGI
                     EGWRWTSVTGMVGLGDFPGAAVESSAEGISRNGQFIVGFGRTEQGLLAFRWTAEEGMV
                     PLGELYGGLFLSWGEVVSDDGQIVAGRSEGQEGHAAFFWTPSFGMVPLEPFLRLFGAD
                     VPEDWILLEVTGITPDGRSLVGNGVNPSGDNEAWIATLPASLDCEPSTCETCVDTDGD
                     GFGDPGIPTNLCPVDACPLDPDPEQTDTDRDGRGDACDACPLDPVNDSDLDGFCSDAD
                     NCPGHFNPDQADADTDGRGDTCDNCPFAENSGQIDQDGDGAGDACDRCPAVPNPGQVD
                     TDADGRGDLCDNCPGAANHLQEDANGDGAGDACQPVVRIDALAPSGGTLFVRAGASDP
                     QGEPLQGRLEIYQETAQDVAISDPAGELSCAIGLLPEGEPGEGIGYAFATTGIPVLFD
                     LDGNLGCVDGLPDFVLGRGRCAGSPAAFDTVLDLSGMSAGDIVCVRAVGGLGPGSELA
                     LAEISPGLLRGRLTSASVLVLSTAFGPGLPRQTDISGLNAGAPHLLKLIATDHHTPPA
                     VGEAPFVPQGENALVINHPPRAAAVAPNAAECDRPGAGSATLDGSASSDADVSPDGDT
                     EIVRYEWIFDPAGPAEAPLGEGAVLQAILPLGSHAVGLRVTDRFGESDATTFAVEVRD
                     TQAPTLSLTAVPNRLWPPNHKRVHVRVSWTAADACDAAPEVMLLSAASSEPDDLPGPS
                     DGVTVGDIFDASPGTPDRDIWLRAERQDGGPGRIYTLTYRVVDAAGQVTTTQTTIVVP
                     VSRWPGVDPTRTYRADRARGITAIDEVP"
     gene            8722..9012
                     /locus_tag="VFD06_07185"
     CDS             8722..9012
                     /locus_tag="VFD06_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HZN03357.1"
                     /translation="MSQETKTPQQGMIQISTGDEMSRGRYSNNMLILHSREEFIMDWL
                     LNSPAGTHLVSRIVVSPGHVKRIIGALVDNLRKYEEKFGPITPPEPKDQAFH"
BASE COUNT         1280 a         3327 c         3158 g         1355 t
ORIGIN      
        1 cagccccgcg ccgacgaacg cggccgcggc ccgcagcgcc tcggtccccg ccttgcaggc
       61 gaactcgaag tcggcggcgc tcgtccacgg cacggcgccg atcgcctccg ccacgacggt
      121 gccgctcggc ttcaccgcgt acggcttcga ctcggtcccg acgaacaccg ccccgagccg
      181 ttccggcgtc accgcgtcgc cggcacgcgc cagggcgtgc cgtgccgctt cgatcgccat
      241 ggtgatggtg tcctcgtcga gccccgggac cgatttctcc ttcaccggca gccgtccctc
      301 gccgttccgc ccccaggccg aggccagcac cttcgccgac acccggccct ccgggacgta
      361 ggcgccgtag ccgagaaccc cgatctcccg ggtcacgcga tcctccgcgc cggccggcgc
      421 ccacgccgcc gcccggttcc gacctccgcc gccgcggcgt cgtcgcgcag cagcccgcgc
      481 aggtagaagt ccgtcatctg tcgggcgagc ccctcttcgt cgaggcgtcc gccggggcgg
      541 aaccactggt aggtccagtt caccatgccg agcagcgcat acgccgcgac ggtcggatcg
      601 accggccgcg ccaccttccg catcacgagt tcccggatgg tcttctcgag gaaacggatg
      661 tagcgcttct tgcgcgcgtc gaggcgggcg cgcgccgagg ccggcagcgc ccccccttcg
      721 tgcaggatga cggtgacctc cttcggccga tcccgcagca cgagcagcag gtggccctgc
      781 acggtggcgc acaggcgcgc aagcggatcg gcgatcggct cggtgcgcga caggacccgc
      841 tcctcgaaca ggtccatgcc gtagtgcatg atcgcggcga gcaggtcctc cttccccgcg
      901 atgtggtgat agaccccggc cttggtcatc ccgaccgccg cagcgacgtc ccccatggac
      961 gtcttctcgt agccccgctc gcagaagacc cgcgccgcct tctccaggat ctgcccctgg
     1021 cgggtggcgt gatcgagacg ggcggcacgg ctcatggcgt tcgagggtct ccggtcggcg
     1081 gcacgaggcc gaagctacct accgaccggt cggtagtatt cacggcgtgg gcttggcgtc
     1141 aagaccccgg tccgcatcgt ggacacccgc cgcttggggc gcatccggtc gcactccccg
     1201 tacactacgc gcccgatgga accggccgga gccgcggcac ccgaggcagc gcgcgaggaa
     1261 gggctcagga agagtctctc cttccgcgac gtgttcctct tcctcgtcac ggccggcgtg
     1321 aatctgcaat gggtcgcgac cgccgccgcc tccggcccga gcgtcatggt ggtgtggttc
     1381 atcggtctcg ccgccatgac ggtgccgctc tcctggtgca ccatccggct cgccgcgcac
     1441 catcccgacg agggggggct gtacgtctgg agccgcaagg cgttcggaga ggcgggcggc
     1501 ttcctgtgcg gctggatcta ttggatgacc aacctgccgt acttccccgg cgtgctctac
     1561 ttcgccgccg gcaacggcct cttcctcgct ggcgcgaact accgccacct gtcctcggac
     1621 cgcggctact tcctcgtctt ctcgctcctg gcgctcggcc tcgccacctg gatgaacctc
     1681 gtcggcctgt cggtcgccaa gcgcctgaac aacctcggag cgcacgcccg ctgggtcggc
     1741 ggcctgctcc tcatcgggct cggggccacc gcctggtggc acttcggctc gatgacgccg
     1801 ttcgaccggg cctcgctgac cccgcgcctc gacctccagg ggctggtctt ctggagcacc
     1861 atcgccttcg cgctcaccgg cctcgagtcg gtctcgttca tgggcggaga gatccacgac
     1921 gcccgcaagg tcgtgccgcg cgccatcgcc gcggcggtgc cgctcatctt cgtcatctat
     1981 tacctcggca cggcggcggt cctggtggct ctgccgccgg gagaggtcag cggactgcag
     2041 ggggtgatcg aggcggtggc ggcgatcgag tcgcggctct ctctccacgg ggtgacccgg
     2101 gtgatggccg ggatcctgct gctgcactcg ctggggagcg tcggcgcctg gctcggggcg
     2161 gtggcgcgga tcccgtacgt cgccgggatc gaccgcttcc tgccgcaggg cttcgcgcgt
     2221 ctccacccgc gctggggaac accgtacgtg gcgcttctga cgcaggcggt cgtgaccgtc
     2281 gtcttcatcg tcatcggcca ggcggggacg agcgtggcgg gcgcgtacga agtgctcgtc
     2341 gggatgatgg tagtgatcta catgctgccg ttcctgttcc tgtacggcgc ggcgctgcgg
     2401 ctgccgctcg accggaccgt cgccgcgcgg atcgcgaccc gatgcctggc gctcctggga
     2461 ctgttcacgg tgaccgcggc gatcgggctc gcgatggtgc cggcggcgca cgagccgaac
     2521 aagccgctcg cgatcctcaa gatcgccggc tcgaccgccg ccatggtggg aagcggcgtg
     2581 ctcgtctatc tcttcaggcg ccctcggctt cgatcgcccg gcgccgccgc gtgacgtagt
     2641 cggagagttc ctcgtcgatc cccgggtcga ggaccggctc cttgtacgac tcgagaaggg
     2701 ttttccagcg cggcgtggcg cgggcggcgg cgtccttgct ccccttcttg acccaggagt
     2761 cccaggcctc cgtgtcggcg agcctcggga aatagaactc gctcttgaag tgcctgcgtg
     2821 tgtgcgcctc cccgaggaaa tgcccgccga ccggcacgtg cgcgatgacg tcgagcgcca
     2881 gccgatcgtc gtccacgagc agccccccga agaagcgctg cgtcatctcg agcacctcgg
     2941 cgtcgaggac cagcgactcg aacgaggcga tgagcccccc gtcgagccag ccggcggcgt
     3001 gcaggacata gtgcacccgc ccgaggagga gcggccagag catgttcatc ttctcctgcc
     3061 ccgcctggtg gtccggcacc ttggagttcg tcaggatgcc gccgccgcgg cacggcagcc
     3121 ggtagcgccg cgccatctgc gccgacgaca ggatcccgag tgctgcctcc gcccggccga
     3181 agccgggggc cccggtgcgc atctcgaggc cggtcaggaa cgacccgtac atcaccgggg
     3241 cgccggggcg caccagctgc gtcagcacca ccgcccccag cgcctcggcg ttctgctgcg
     3301 ccaccgccgc cgccagcgtc accgggctcg tcgccccggc catgatgaac ggcgtgatga
     3361 tgttcacctg ccccgcctcg gcgtactcga ggagcccctc gagcatcggc gtgtcgtaac
     3421 gcagcggcga gttcgagttg atgatgccga cgaggaccgc ccggtcgcgg atcgcgtcgc
     3481 gcagctcccc gcccgagcct ccggaaccga tcccggcctt ccggtcgagg ccgcggaaca
     3541 ggatccccat gagggcgatg tggtcgcgcg cccgcaccgt gctccaggcg tcgccgatca
     3601 tccccttgcg gctcaggcgg atctggtggt ggaggatgtc gaggtggcgg ctctcgaccg
     3661 agagatcctt gcaggcgacc tccggcgtgc cgtgatgcag cacgtcgagc gtctccgcca
     3721 ggcgcacgag atcgttctgg tccggaaggg tgccgtcgcg ccggccccga tcggcgtccg
     3781 ccacgaaggg cgggccgctc acgggggcga agatcaccgt gtcgtcgccg acggtgacgt
     3841 tccagtccgg atcgcgcgcg tgcaggacga actgcgccgg cgctttcgcc gcggtctcga
     3901 tgacgaaatc gggatcgaag aagacccgct gcgcgccgtc atcgatcttc tggccggcgc
     3961 ggcggaacag atcgaccgcg cgcggctcct cgaacaccat cccctgctcc tggaggagac
     4021 gcatcgaggc gcggtgcacc tgctcctcgg actcggggct cagcaggtcg agcggcttga
     4081 gcggattgcg aaaaccgcgc acgatttcct ctccgaaacc ggatgagccg gcagactaac
     4141 acagccgcgc gcgcgttttc gtcagcgacg ctgcccgcac ggcgcgtctt gaccgcgtcg
     4201 gatcccggac ctatatctgg agcgtcgttc ccccgaagtc cgttgggagc cagcaacagc
     4261 agtccgcaac acccgcaacg ccgaggatga catcgccgat gccacgctca ggtcacgtcc
     4321 gccgcccgca ccgatccgca ccgcgcgccc tgctggcgct ggcgatcctc gtgacgagcg
     4381 cgctgtccgg gtcgcctctc cgggccggcg caccggatca ggacgagccg cgcctcgtgc
     4441 gcgccgagcc gggcggcttc gtggtggcgc cgcaggcggg ggacgtggcg ctcgaccttt
     4501 acggcgagaa cctgtgggac gccgatcgcc tgtggaccga gcgcaacgtg cgggtctacg
     4561 cgcggcacgc ctccggcgac gaggcgtgga cgcgcctctt caccgggtcc ggagacgccg
     4621 gcaacggcgc cccggaggac gcgtccgacg ctttggcgcg cgggcgggtc gtcgagtcgc
     4681 tcccggatcg gtaccgcttc cttctgccgg cctccctgtg gctcgaccgc aaggggacgc
     4741 tcgagttcaa ggtggtgaag gggacctggc gccgcgcggg agaggggcgc tggcgctacc
     4801 aggagacggc cgcctcgaac atcttgaagc tgccggtggc cgaggccccg caccgcgctc
     4861 cggaagtgcg cgagatctcc ccggcccttc tcccggcccg gcacgacggc ggccccccgc
     4921 cgaccctgtg ggtccgagcc tccgatctga cgctcgatcc ggaggtgcgt atcggcggcg
     4981 tgccctgtcc aacagtgagg atcgacgtct cgctcgactt catcgagtgc cgcgtccccg
     5041 gcgacatcct cgatcacccg gacacctatc aggtgacggt gcgcacgccc gtcggcgagg
     5101 cggcggaggg gctgtcgctt tcggtcgtct cgccgccggc catcgcggcg ctttcgcccg
     5161 aggcgctgcc tgcgaagggg ccggacgccc tcgtcgtcgt ccgcttctcc gggtccgagc
     5221 cggacaccgc ccggatgcgc cggatcgatc gcggcggctg gacgaccgtg gcgctccggc
     5281 acctcgagcc gggggcggcg cgcctggagc tgccggcggc cctgcgcggt ttcgcgggag
     5341 agatcgaagt ggagttgggg aacctcgccg ggacggcgcg ggccagcctg gcgctgtgcg
     5401 ccgaggaggg gccgaaccct tccgtctgct cgcgcgctgg gacgctcgcc ggccccggac
     5461 ctgaggtcgc cgccgcggcc ctccgccgtc cttcaggatc cgcgcgtccc gccggcatcg
     5521 agcccgcctc ccccgctccc gctcagggga cttcgtcgat ggccgtgatc cccctggccc
     5581 gatcggcgcg gtaagttctc gtcggatcga cccccggcca gcggctcacc ggcacgacga
     5641 tcgtcgtctg cgtggtggtg acctgacccg ccgcatcgac gacgcgatag gtgagcgtgt
     5701 agatccgtcc cggtcccccg tcctgccgct cggcccgcag ccagatgtcc cggtccggag
     5761 ttccggggga cgcgtcgaag atgtcgccga ccgtgacgcc gtcggaggga cccggcaggt
     5821 cgtccggctc gctgctggcg gccgacagca gcatcacttc gggcgccgca tcgcaggcgt
     5881 cggcggcggt ccatgacacc cgcacgtgca cgcgcttgtg attcggcggc cagaggcgat
     5941 tcggcacggc ggtgagggac agagtggggg cctgcgtgtc ccgcacctcg accgcgaagg
     6001 tcgtggcgtc ggactcgccg aagcgatcgg tcacccgcag cccgaccgca tgcgatccga
     6061 gcggcaggat cgcctggagg accgccccct cgccgagcgg agcctccgcc ggacctgcgg
     6121 gatcgaaaat ccactcgtag cggacgatct ccgtgtcgcc gtccggcgag acatccgcat
     6181 cggacgaggc ggaaccgtcg agcgtcgccg atcccgcccc aggccggtcg cattccgcgg
     6241 cgttcggagc gaccgccgcg gcgcgcggcg ggtggttgat gacgagcgcg ttctccccct
     6301 gtggcacgaa gggggcctct ccgaccgccg gcggggtgtg gtggtcggtc gcgatgagct
     6361 tcaggagatg cggcgcgccc gcgttgaggc cggagatgtc ggtctgccgc ggcaggccgg
     6421 ggccgaacgc ggtcgacagg acgaggacgc tcgcgctcgt cagccggccg cgcaggaggc
     6481 cgggggagat ctccgccagg gccagctcgg accccggtcc caatccgccg accgcccgga
     6541 cgcagacgat gtcgccggcg gacatccccg agagatcgag gacggtgtcg aacgccgcgg
     6601 gcgaaccggc acagcgacct cggcccagga cgaaatccgg cagaccgtcc acgcagccga
     6661 ggttgccgtc gagatcgaag aggaccggga ttccggttgt ggcgaaggcg tacccaatcc
     6721 cctcccccgg ctccccctcc ggcagcaagc cgatcgcgca ggacaactcc ccggccgggt
     6781 cggagatcgc cacgtcctgc gccgtctcct ggtagatctc cagccggcct tggagcggct
     6841 cgccctgcgg atcggaggcg ccggcccgga cgaacagcgt cccgcccgac ggggccaacg
     6901 cgtcgatgcg cacgacgggc tggcaggcat ccccggcgcc atcgccattc gcatcttcct
     6961 gaaggtgatt ggcggcgccg gggcagttgt cgcagagatc gccgcgcccg tcggcgtcgg
     7021 tgtcgacctg gcccggattg gggaccgccg ggcagcgatc gcaggcatct cccgcgccgt
     7081 ccccgtcctg gtcgatctgg ccggagttct ccgcgaaggg gcagttgtcg caggtgtcgc
     7141 cgcggccgtc ggtgtcggcg tcggcctggt ccgggttgaa atgccccgga cagttgtcgg
     7201 cgtcgctgca gaacccgtcg aggtcggagt cgttcaccgg gtcgagcgga caggcatcgc
     7261 aggcatcgcc gcggccgtcg cggtcggtgt cggtctgctc cggatcgggg tcgagcgggc
     7321 aggcgtcgac gggacagaga ttggtcggaa ttccggggtc gccgaagccg tcgccgtcgg
     7381 tgtcgacgca ggtctcgcag gtcgaaggtt cgcagtcgag cgaggccggc agtgtcgcga
     7441 tccacgcctc gttgtcgccg gacgggttga cgccgttgcc gacgaggctg cggccgtccg
     7501 gggtgatgcc ggtcacctcg agaaggatcc agtcctcggg gacgtccgcg ccgaagagtc
     7561 gaaggaacgg ctcgagcggc accatgccga acgagggagt ccagaagaag gccgcgtgcc
     7621 cctcctgacc ttcggacctt cccgcgacga tctgcccgtc gtccgacacc acctcgcccc
     7681 agctgaggaa cagcccccca tacagctcgc cgagcggcac catcccttcc tctgccgtcc
     7741 agcgaaacgc cagcaggcct tgctcggtgc gcccgaaccc gacgatgaac tggccattcc
     7801 tcgagatccc ctcggcggag ctctccactg cggccccggg gaaatccccc agccccacca
     7861 tgccggtgac ggaggtccaa cgccacccct cgatcccggc cgcggaattg ccgtggccga
     7921 tgatgaccga gccgtcgccg gagatgccga gcgcgttgct ggcgaatacc ccccccggga
     7981 gatcgccgag cggctgcatg ccggtcggca gcgtccagcg gtacgcctcg atgccgaacg
     8041 ggccgaagct ctggccgacg atgacgctgc cgtcggaggt ggtgtcggtg gcgatgctgt
     8101 tctccggtcc gcccgggaga tcaccgaggg ggatcaggcc ggtggcctcg gaccagcggt
     8161 aggccaggaa cacggtctgg gcgagctgac gcgacccgac gatgaccgat ccatcgtcgg
     8221 acacggagtc cgccaccgcc ccgaccggtc cgaccgtgac cggaccgagg ctctggggcc
     8281 cgcccgggag gacccagcgg cacgcctcgc ggccggtgtt ccccgacgac tgcccgacga
     8341 cgacccggcc gtcggccgac atcccgtagg cctggctgtg gaagacgccc ccgggcaggt
     8401 cgccgaggcc gcggaaggtc gcgctcgccg agtcgggagg gttcggaggg ttcggaacgg
     8461 cgggggcggg ccccgccatc atcagaagag cgggaacgat gacgtggtat cccgccacga
     8521 cccagaccgg tcccgtccgg cgcatcgcgt ctccccctga agaacgggcc ggagcgtacg
     8581 gccagaatcc cggcggtgcc gcatcaacct cgcgggcggc ccactcgccg ctgcggctcc
     8641 acagcaacgg cgttgccggt atgatgcggg ggctcgcacc agcacatccg ctcgcaccca
     8701 cacctcgagg aggcggcccg catgtcccag gaaaccaaga ctccccagca ggggatgatt
     8761 cagatcagca ccggggacga gatgtcccgc ggccgctaca gcaacaacat gctcatcctg
     8821 cacagccgcg aggagttcat catggactgg ctcctcaact ccccggccgg aacacacctc
     8881 gtctcccgca tcgtcgtcag ccccggacac gtcaagcgga tcatcggggc gctcgtcgac
     8941 aacctgcgca agtacgagga gaagttcggc ccgatcacac ctcccgagcc gaaggaccag
     9001 gcgttccact gaaattccgc gaaggaaaag gctgacgccg gcgcctgcct tcggtatcat
     9061 tgaggtttcc ggcaaccgcg ggtcgggggg cccgtctctc gcctcggggg gagcatgagc
//
LOCUS       DASRIQ010000296         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_897417, whole genome shotgun sequence.
ACCESSION   DASRIQ010000296 DASRIQ010000000
VERSION     DASRIQ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897417"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1008)
                     /locus_tag="VFD06_07190"
     CDS             complement(52..1008)
                     /locus_tag="VFD06_07190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN03358.1"
                     /translation="MRVVTADLCIVVPAYNEEKNLSVLVEELGQALGPAGIGFEVLFV
                     DDGSQDETAAVLRRLTASHKNIRAIILSRNFGHQAAVSVGLQHARGSAVAVMDADLQD
                     RPDDLVQLYRRFREGADVVYAVRRSRPEGLAKRLAYAVFYRLMRRLTTVPIAVDSGDF
                     CVMSAETVSRLNALPETQRFVRGLRAWVGGRQVGVAVDRDPRRAGRPQYTFVKLCRLA
                     IDGLVSFSYVPLRLASLLGFAIAGAAIVGIVVVLAWKVMGRLPTGAGVATIALSVLFI
                     GAVQLLTLGILGEYVGRIFDEVKARPVAVVSEVLGEDRQQRA"
     gene            complement(1087..>2241)
                     /locus_tag="VFD06_07195"
     CDS             complement(1087..>2241)
                     /locus_tag="VFD06_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN03359.1"
                     /translation="LPSHASLFTSLLPFDMRRHWDYGGTVHLARVLLAERLYEAGYRT
                     AGFTGGGYVSSQFGFEQGFEVYEDHDETLEGGPEAIAEAALSWVRSVKGAPFFLFLHT
                     YEPHYPYVHADFAKADDAGRLPRIVSGKEVEEMHRGRLVPTPAERRYVTDLYDGDVAH
                     ADSVIGGFLEALRGDGTLDRTLVVVLSDHGEELWDREPGYSPDHGHSLYQELVHVPLL
                     FRWPGQVRAGARIRTPVSLLDVAPTLLDLAGLPADPLHEGRSLATTLRTGGEPDPRTI
                     VVESVQYGPDRFLMRTGDLAAILTPYPDRLDNAVSIPARALEVFDLAVDPLEQHDLSS
                     HLTGTAAEMVDALWQRSRGILNSPSRKGAEEAPELPEELLEQLRSLGYLR"
BASE COUNT          364 a          762 c          800 g          315 t
ORIGIN      
        1 gtcccaggcc agatcgctca gacggtacgc caccgaacgc tcgtcactcc ctcaggcgcg
       61 ctgctgtcga tcctccccca ggacctcgct gacgacggcc acgggtctcg ccttgacctc
      121 gtcgaagatg cggccgacgt actccccgag gatccccagg gtgaggagct gcacggcgcc
      181 gatgaagagg acggagagcg cgatggtggc cactccggcg ccggtcggga gccgccccat
      241 gaccttccag gccaggacga ccacgatccc gacgatcgcg gcaccggcga tggcgaagcc
      301 gagcagggag gcgaggcgca gcggcacgta ggagaacgag accaggccgt cgatcgccag
      361 gcggcagagc ttgacgaagg tgtactgggg acgcccggcg cggcgcggat cgcgatcgac
      421 ggcgaccccg acctgccgcc ctccgaccca ggcccgcagg cccctgacga agcgctgcgt
      481 ctccggcagc gcgttcaggc gcgacaccgt ctcggcgctc atgacgcaga aatcccccga
      541 gtcgaccgcg atcgggaccg tggtcagccg gcgcatcagc cggtagaaga cggcgtaggc
      601 gagccgcttg gcgagtccct ccggccgcga gcggcggacg gcgtagacga cgtcggcccc
      661 ctcccggaag cggcgataga gctggacgag atcgtccggg cgatcctgga ggtcggcgtc
      721 catgacggcg acggcgctgc cgcgcgcgtg ctggaggccg accgagaccg cggcctgatg
      781 gccgaaattc cggctcagga tgatggcccg gatgttcttg tggctggccg tgagccggcg
      841 caggaccgcg gcggtctcgt cctggctccc gtcgtcgacg aagagcacct cgaatccgat
      901 gccggccgga cccagggcct ggccgagctc ctccaccagg accgagaggt tcttctcttc
      961 gttgtacgcc ggcaccacga tgcagagatc ggccgtcacc acccgcacct gtcgcccgcg
     1021 tcgcccgggg ctggatcccg cgcgcacggt cttcgcgcgc gcgggtggcg catcatacct
     1081 cggccgctag cgcaagtagc cgagggagcg cagctgctcg aggagctctt ccgggagctc
     1141 cggagcctcc tctgcccctt ttcttgaggg ggaattcagg atcccccgcg agcgctgcca
     1201 cagggcatcg accatctccg ccgcggtccc agtcaggtgg gaggagaggt cgtgctgctc
     1261 caggggatcg acggccaggt cgaacacctc gagcgcccgg gccgggatgg agacggcgtt
     1321 gtcgagccga tccgggtagg gagtcaggat cgccgcgagg tctccggtcc gcatcaggaa
     1381 gcgatcgggg ccgtactgca ccgattcgac cacgatcgtc cggggatcgg gttcaccgcc
     1441 tgtcctcagg gtcgtcgcga ggctccggcc ttcgtggagc ggatccgccg ggagccccgc
     1501 gaggtcgagc agggtcggcg cgacgtcgag gaggctcacg ggcgtccgga tccgcgcgcc
     1561 agcgcggact tgcccgggcc agcgaaagag cagaggaacg tggaccagct cctggtacag
     1621 ggaatggcca tggtccgggc tgtagccggg ctccctgtcc cacagctcct ctccgtggtc
     1681 cgagaggacc acgacgagcg tccggtcgag ggtgccgtct cctcgcaggg cctcgagaaa
     1741 accgccgatg acgctatcgg catgagcaac gtccccgtcg tagagatcgg tcacgtagcg
     1801 cctttcggca ggtgttggga ccaggcggcc gcggtgcatc tcctcgacct cctttccgga
     1861 cacgatccgc ggcaggcggc cggcgtcgtc ggccttcgcg aaatcggcgt gcacgtaggg
     1921 gtagtgcggc tcgtaagtgt ggaggaagag gaagaagggg gctcccttga ccgagcggac
     1981 ccaggaaagc gccgcttcgg cgatggcctc cgggccgccc tcgagcgtct cgtcgtggtc
     2041 ctcgtagacc tcgaatccct gctcgaagcc gaactgcgag gagacgtagc cgcctccggt
     2101 gaaccccgca gtccggtagc cggcctcgta caggcgctcc gccaggagga ccctcgcgag
     2161 atggaccgtc ccgccgtagt cccagtgccg gcgcatgtcg aagggcaaga gtgaggtgaa
     2221 cagcgaggcg tgcgacggga g
//
LOCUS       DASRIQ010000297         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_650329, whole genome shotgun sequence.
ACCESSION   DASRIQ010000297 DASRIQ010000000
VERSION     DASRIQ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650329"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..595
                     /locus_tag="VFD06_07200"
     CDS             131..595
                     /locus_tag="VFD06_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HZN03360.1"
                     /translation="MVEDSGQAEAAQTVPLLEERLAVERRVVETGRVRVRLSTATKDA
                     MVRETLRGERVEVDRVPVGREVTEAPVVREEENGTVLVVPVLEEVLVVERRLVLKEEL
                     RIRRVSTTEIIEQTVALRQQAATVERLLTAVPQDGSDPETRRRDVGDDGDDR"
     gene            592..1497
                     /locus_tag="VFD06_07205"
     CDS             592..1497
                     /locus_tag="VFD06_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015857237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HZN03361.1"
                     /translation="MTLMTLTALFDTRAEADRAGERLVSEVGIARSDVTVIAQEAGAA
                     GSGVAEDTGFFGALKSLFMPDDDRDAYSEAIRRGGFLLTARVEEGGAEQAMAILEADG
                     AVDLDERRQTWRAEGGRTGGDEAPSVAQALPGGTVVATGAAGAAGGTPAAAARPAGAG
                     EEETIALAEERLRVGKREAERGRVRVRSYVIETPVEEQVRLREERVDVERRPADRPVA
                     DADEALFRERTVEATETAEEAVARKDAHVTEEVVVRKEAEERTEAVRDTVRRTEVEVE
                     DDRRRAGTALPAAAEAPRERDRDRS"
     gene            1595..2125
                     /locus_tag="VFD06_07210"
     CDS             1595..2125
                     /locus_tag="VFD06_07210"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HZN03362.1"
                     /translation="MLVEQLKDAYSAEKQALRCMQRTLRKASAPELRDGIQAHIEQTE
                     TQIDRVEQALERLDARPGRKVCEAMRGLVEEAQHEAEEHDKGPILDLVIAAGMQRIEH
                     YEIAAYGTAVAVAEAIGEREVASLLSTTLEEEKQTDLKLTAVTAKHVMPAALQGGENE
                     NEPAGRGAMAARRRAS"
BASE COUNT          344 a          722 c          869 g          263 t
ORIGIN      
        1 gcggaagcgg cgggggatct gcgcacagcg gctttcgatt tctgcatagc cccgctcgcc
       61 cggacgccgc gttgggtgct gagggtgctc ggccggatgc agggccggcg tccgcaaccg
      121 gggtggagtc atggtggaag acagcgggca ggccgaggcg gcccaaaccg ttccgctgct
      181 cgaggagcgc ctcgccgtcg agcggcgcgt cgtcgagacc ggtcgcgtgc gcgttcgcct
      241 ctcgaccgca actaaggacg cgatggtgcg ggagaccctg cgcggcgagc gcgtcgaggt
      301 cgaccgcgtg ccggtcggcc gcgaggtgac cgaagcgccg gtggtgcggg aggaggaaaa
      361 cggcacggtc ctcgtcgtgc ccgtgctgga ggaggtgctg gtggtcgagc gccgcctcgt
      421 cctcaaggag gagctgcgga tccgccgcgt ctccacgacc gagatcatcg agcagaccgt
      481 cgccctccgc cagcaggcgg cgacggtgga gcggctgctg acggccgtcc cgcaggacgg
      541 ctccgatccc gaaacccgcc gacgcgacgt cggcgatgat ggagacgaca gatgaccctg
      601 atgaccctga ctgccctgtt cgacacgcgc gccgaggccg accgggccgg cgagcggctg
      661 gtgagcgagg tcgggatcgc ccgatcggac gtcaccgtga tcgcccagga ggcgggcgcc
      721 gccgggagcg gcgtcgccga ggacacgggc ttcttcggcg cgctaaagag cctgttcatg
      781 cccgacgacg accgcgacgc ctattccgag gcaatccggc gcggcggctt cctgctgacc
      841 gcacgggtcg aggagggagg cgccgagcag gcgatggcga tcctcgaggc ggacggcgcg
      901 gtggacctcg acgaacgccg gcagacctgg cgggcggagg gcggccgcac cggcggcgac
      961 gaggcgccgt ccgtggcgca ggccctgccc ggcggcaccg tcgtcgcgac cggcgccgcg
     1021 ggcgccgccg gcggcacgcc cgcggccgcg gcgcgccctg ccggggccgg cgaggaagag
     1081 accatcgccc tcgccgagga gcggctgcgg gtcggcaagc gcgaggccga acgcggtcgc
     1141 gtccgggtcc gcagctacgt catcgagacc ccggtcgagg agcaggtgcg gctgcgcgag
     1201 gagcgcgtgg acgtggagcg ccggcccgcc gatcgcccgg ttgccgacgc cgacgaggcc
     1261 ctgttccggg agcgcacggt cgaggcgacc gagaccgccg aggaggcggt ggcgcggaag
     1321 gacgcgcacg tgacggagga ggtggtggtg cgcaaggagg cggaggagcg gaccgaggcg
     1381 gtgcgcgaca ccgtccgccg gaccgaggtg gaggtggagg acgatcgtcg ccgcgctggc
     1441 acggcgctcc ccgctgcagc cgaagcgccg cgtgagcgcg atcgcgatcg gagctgacag
     1501 gcgggccgcc ttcgagcggc ccgcttcccc gttccgccaa tcaacaagtg tgtttttcga
     1561 tcaaggagtt tccatgagac acgaggttcg cgagttgctg gtcgagcagc tgaaggatgc
     1621 ctacagcgcc gagaagcagg cgctgcggtg catgcagcgg accctgcgca aggccagcgc
     1681 gccggagttg cgtgacggca tccaggccca tatcgagcag accgagacgc agatcgaccg
     1741 cgtcgagcag gcgctcgaga ggctcgacgc gcgtccgggc cggaaggtct gcgaagctat
     1801 gcgcggcctc gtcgaagagg cgcagcacga ggcggaggaa cacgacaagg gtccgattct
     1861 ggacctggtc atcgccgccg gcatgcagcg gatcgagcac tacgaaattg cggcctatgg
     1921 caccgcggtg gcggtcgccg aggcaattgg cgagcgcgag gttgcgtccc tgctctcgac
     1981 tacgctggaa gaggagaagc agaccgacct caagctgacc gcggtgaccg ccaagcacgt
     2041 catgccggcg gcgctgcagg gcggcgagaa cgagaacgag cccgccgggc gcggcgccat
     2101 ggcggcgagg cgccgcgcct cctgatcgag cggtcgccgg ccgttgcgtt cgcggcccca
     2161 ctgagtctca gtggggccgc ggccttcggg gatagcgt
//
LOCUS       DASRIQ010000298         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_119057, whole genome shotgun sequence.
ACCESSION   DASRIQ010000298 DASRIQ010000000
VERSION     DASRIQ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119057"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFD06_07215"
     CDS             <1..956
                     /locus_tag="VFD06_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN03363.1"
                     /translation="WEGNYSSWLDQKRTRLAQEEKQAGARQRTLERELEWVRMAPRAR
                     QAKGKARLAAYEKLLAEETEKAPDSVEIYIPPGPRLGGVVVEAEGVEKAYGDNLLFEN
                     LTFKLPRGGLVGVIGPNGAGKTTLFRMITGQEKPDHGTLRIGDTVTLGYVDQSRDALD
                     PAKNVFEAISEGNEMIELGKRQVNARSYVASFNFKGVDQQKRIKDLSGGERNRVHLAR
                     TLKTGANLLLLDEPTNDLDVDTLRALEEALLEFAGCAVVISHDRWFLDRIATHILAFE
                     GDSKAVWFEGNYQDYEADRKKRLGADADQPHRIKYKRLTHD"
     gene            complement(928..1602)
                     /locus_tag="VFD06_07220"
     CDS             complement(928..1602)
                     /locus_tag="VFD06_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HZN03364.1"
                     /translation="MRVVALCDSLPAAARLWRRLDGMPEVDLRVLVIGGPLRRVRGSA
                     AVDWRTAARLLVTGRLFFAMRGLDHPSAVARLRALRPAIGLCACGTICRKPLIDCFER
                     GILSPHPGLLPEYRGRAAMEWSILEGRPTGITVFFLDPGIGTGKEIVLRREVRVAPAP
                     DLPAARRRLAERDAEFFREALERLRSPAYRPLENHGRGRRFYAMSRLFTGVVEQALNR
                     ASGACT"
     gene            complement(1599..2018)
                     /locus_tag="VFD06_07225"
     CDS             complement(1599..2018)
                     /locus_tag="VFD06_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03365.1"
                     /translation="MMGGAALRPLLQAALVRRRRELDDLLKEPAGAAARYPIPARGEN
                     GDPEETLRRLMVVLCPAVTRAPLDGTARDAYLSQVVRLEQAAQSADPRFLDAWNHAYE
                     AIASWPAPLREEGAARFTLERYAVLLDHHLAGIAGAA"
BASE COUNT          354 a          725 c          764 g          235 t
ORIGIN      
        1 catgggaggg gaactactcc tcctggctcg accagaagag gacgcgcctg gcgcaggagg
       61 agaagcaggc cggggcgcgg cagcgcaccc tcgagcgcga gctcgagtgg gtgcgcatgg
      121 cgccgcgggc gcgccaggcc aaggggaagg cgcgcctcgc cgcctacgag aagctcctcg
      181 ccgaggagac cgagaaggcg ccggactcgg tggagatcta catcccgccc gggccgcgcc
      241 tcggcggcgt ggtggtcgag gcggaggggg tggagaaggc gtacggcgac aacctgctgt
      301 tcgagaacct gaccttcaag ctgccgcgcg gcggactggt cggtgtcatc ggaccgaacg
      361 gcgccggcaa gacgaccctc ttccggatga tcaccggcca ggagaagccg gaccacggca
      421 cgctccggat cggcgacacc gtcaccctcg gctacgtcga tcagtcacgc gacgcgctcg
      481 accccgccaa gaacgtcttc gaggcgatca gcgaggggaa cgagatgatc gagctgggga
      541 agcgccaggt gaacgcgcgc tcgtacgtcg cctcgttcaa cttcaagggg gtcgatcagc
      601 agaagaggat caaggacctc tccggcggcg agcgcaaccg cgtgcacctc gcccgcaccc
      661 tgaagacggg ggccaatctc ctgctgctcg acgagccgac caacgacctc gacgtcgaca
      721 cgctgcgggc cctcgaagag gcgctcctcg agttcgccgg ctgcgccgtc gtcatcagcc
      781 acgaccgctg gttcctcgat cggatcgcca cgcacatcct ggcgttcgag ggggacagca
      841 aggcggtctg gttcgagggg aactaccagg actacgaggc ggatcgcaag aagcgcctcg
      901 gcgccgacgc cgaccagccg caccgcatca agtacaagcg cctgacgcac gattgagagc
      961 ctgctcgacg acgccggtga acaaccgcga catggcgtag aagcgccgcc cgcggccgtg
     1021 gttctcgagc ggccggtagg ccgggctccg cagccgctcc agggcctccc ggaagaactc
     1081 cgcgtcgcgc tcggcgagcc tgcgccgcgc cgcgggaaga tccggcgcgg gcgcgacgcg
     1141 cacctcgcgg cgcaggacga tctcctttcc ggtgccgatg ccggggtcga ggaagaagac
     1201 cgtgatcccg gtcgggcgcc cctcgaggat cgaccattcc atcgccgcgc ggccgcgata
     1261 ctcgggcagg agccccgggt gcgggctcag gatgccgcgt tcgaagcagt cgatgagcgg
     1321 cttgcggcag atcgtcccgc aggcacacag cccgatcgcc ggccgcaacg cgcgcaggcg
     1381 ggcgacggcc gacggatgat cgagaccgcg catcgcgaag aagaggcgtc cggtcacgag
     1441 gaggcgcgcc gccgtgcgcc aatccaccgc cgcgctgccg cgcacccggc ggagagggcc
     1501 tccgatcacc aggacgcgca ggtccacctc cggcatcccg tcgaggcggc gccacaggcg
     1561 ggccgccgcg ggcagggaat cacacagcgc gacgaccctc acgcggcacc ggcgatcccc
     1621 gcgagatggt gatcgaggag cacggcgtag cgctcgagcg tgaagcgggc cgccccctcc
     1681 tcgcgcaagg gggcgggcca cgaggcgatc gcctcgtaag cgtggttcca ggcgtcgagg
     1741 aaacggggat cggcgctctg ggccgcctgc tcgaggcgga cgacctgcga caggtaggcg
     1801 tcacgcgccg tgccgtcgag aggggcgcgg gtcacggccg ggcacaggac caccatgagg
     1861 cgccgtagcg tctcctccgg atcgccgttc tcgccgcggg cgggaatcgg ataccgcgcg
     1921 gccgcgccgg ccggctcctt gaggaggtcg tccagctcgc ggcgccggcg cacgagcgcc
     1981 gcctggagga gggggcggag cgcggccccg cccatcacgc ggcccgtgcc cggaccggat
     2041 agaagccggc ggcgagatcc tgcgcgatgc gtccgagg
//
LOCUS       DASRIQ010000299        10426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2453535, whole genome shotgun sequence.
ACCESSION   DASRIQ010000299 DASRIQ010000000
VERSION     DASRIQ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2453535"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..2272
                     /locus_tag="VFD06_07230"
     CDS             86..2272
                     /locus_tag="VFD06_07230"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN03366.1"
                     /translation="MRCERCGAPLPASGWTTIPGAKPDLRAASLEFAPGSDFAERFTI
                     IERTGEGGMGVVYKAIDRRLDHTVALKLIQPTIASHPEAIRRFKSEVLLARQISHPNV
                     CRVHDLGESTGILYLSMAWVEGETLHRFLAQSGTMTPAAALGIVERVARALEAAHARR
                     VVHRDLKPDNIMIDAGGEICVMDFGLATVTGDAAKSGGRIVGTPPYMSPEQRRGEEAD
                     PRSDLYALGIILWELLAGRLPSSPLDPASVKSDVPRPLASILASLLAEDRGARCASAT
                     VLLAAIAEARERFPELGKRSPIPRRRRWRAVAAAAVLAGAALVYVAVRPHLDARPPRA
                     PTITTVPPETAGFFERGLYYLRDEGETARGLGDAIQMLHRAVGKTPEDGRAWAVLGEA
                     YWVRFRQTGAPEAKAEAERALARATTLAPHLPETLHARGLGTLVAGDAGAARADFEKA
                     TQLAPDFDSAWESLGNADRDLGNYAEGLRELQTAVRLRPTSYLHQIALGRFFQRFAEY
                     DAAAAAYRKAIDLKPDQPWGWNNLGAMYLFRARRPEEAVPAFKRSIALEDRGIARTNL
                     GTAYFYMGNYEEALAQYRRATELESEDADNWGNVGDALYMLRRPEEARRAYQRGVALA
                     QAGVTARPLDAGSRNQLAKYCAKAGRADCALEEARRAAEMQPDNPDMDFTNALAYAAS
                     GKLTEALDWLEKGVKHGLGRAQIEYEPFFAPLHASARYRAILAQAV"
     gene            complement(2290..2658)
                     /locus_tag="VFD06_07235"
     CDS             complement(2290..2658)
                     /locus_tag="VFD06_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03367.1"
                     /translation="MADIQVVFKTGMPAMVLQDPESVMQNEILNWHFHTAGTPVRAVR
                     ISFEESTATFFPRPALPPTNQMKKIVAHRHTIWGQAPNYFTSGGPQSRPDKYTIEAFD
                     EYDNKLEWATLDPTIRTDGP"
     gene            3144..3749
                     /locus_tag="VFD06_07240"
     CDS             3144..3749
                     /locus_tag="VFD06_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN03368.1"
                     /translation="MGVGTTMSDIDGKSGESGPDRVLRYLDGNAYVYARTQAALIARC
                     GGYDIPSSEQADIVQTVLYEACQAVARPDFKLRKEFGHFIRTLAHRRCIDWWRRNRPN
                     VPMELDRPSLSPLPDEKILANERLALATEIVNQLPPTCRELIEKHIFAGLPYAEIAAT
                     TGRTEGALRVEMCKCLEKARQLARRLLLQPGSRGGGQGDLR"
     gene            3746..4600
                     /locus_tag="VFD06_07245"
     CDS             3746..4600
                     /locus_tag="VFD06_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN03369.1"
                     /translation="MTHDEAVLLLTDLLSGRLAPRVRQDVQGHLEGCSECASLVGTHE
                     MLAGRHTPVEDLVDLSLQPDRIENARRMRLQAHLDVCDACGAEARLVLKAEREAATDA
                     TGDPRGSGSLLLGAAATALLGLGLSAYLGLYRIPRLESTIDRLEAVPPRRIESAPQPH
                     EPGPMVRIQYLPSARRGPGSDAMVSLDPGQPYQYLAVAVPEFEPRGTYTVDLTDPEGH
                     QVWSRTVPAEELRRLTKGTPEITLAVPSRALHEGRFVLRLRGPSGPTAGPTAGPLLET
                     AFEVRPAP"
     gene            complement(4614..5543)
                     /locus_tag="VFD06_07250"
     CDS             complement(4614..5543)
                     /locus_tag="VFD06_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN03370.1"
                     /translation="MSVVRLSILLAAACLTAAAAPAIKAPAPEKGEMLEIAPGLSIFY
                     EVKGQGSGTPLFVVNGGPGFDHAYLHCSDAWDRLAAGRRVVFYDQRGNGRSTPLKDDQ
                     TTKLLDQIADLDALRTHLGAQKFDLLGHSWGGYLVMAYAARHPERIQHLVIADSAAPR
                     IQETAFLFKHIYPETTERQHALAFAEALGDPEAIARSLREYSSMLFVSNEVRDAYLLR
                     SDSFVFRPKINQALWGEAERYDLNPELLKYRFPTLVATGRYDFNVAPSVAYAIHQKVP
                     GSEFAVFEKSGHLPFCEESDAWVKRIEAFLRGK"
     gene            complement(5555..6211)
                     /locus_tag="VFD06_07255"
     CDS             complement(5555..6211)
                     /locus_tag="VFD06_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03371.1"
                     /translation="MTDAPDGRARRLPRFTFLWAFLLGIVVLTLLRPCLRRVPAPPPV
                     QGSFPAVRLIAADGAAIDPASLKGSAWVVSIESVPCLEACAERRGRMAELKDALAGFH
                     LGGIRLLSLEVDTARPAEPEPDHMDSVEADPDQPMEAPPAPPPAGPADPAPPLWIAAR
                     LDEKDLQAFGGLSRLGTLLLVDATGGLRGWYGADQDGFNEIYNRAQHVRSADPDGKVR
                     "
     gene            complement(6208..8883)
                     /locus_tag="VFD06_07260"
     CDS             complement(6208..8883)
                     /locus_tag="VFD06_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN03372.1"
                     /translation="MTLLPGSRVGPYEIRGLIGSGGMGEVYRAVDPRLGREVALKILP
                     ADVGADPDRLGRFRREAQVLASLNHPNIAAIHGLEDAGGIPCLVLELVEGEDLAQRLA
                     RGPIPHEEAIEIARRISEALEEAHEKGIVHRDLKPANVKVTPDGKVKVLDFGLAKAFT
                     AAEAPVAGSDLSRSPTLVQSGTRAGMILGTAAYMAPEQARGRAVDRRADIWAFGAVLW
                     EMLAGRPLFTGETLTDVLAAVLTREPDWTALPPDTPPRVAALLRRCLERDPRRRLQAI
                     GEARLALEPGSGVPDAAAAPAGPATSRAGISRRTAVGWALLAAGAGVAATLLLAPRRE
                     PPPSSTQRLEFGFDPPAGHTFIGGVDLSRDARSLAFVARDPAGQTALWVRDLGEVEPR
                     RIAGTEGARFPFWAPDGRQIGFFAGSQLKSVDLVGGAVRSLVRTTNTSDGRGATWGAG
                     DVIIFAPTFYGGLRSMSAGGGTASPATVLEGKEGTHRWPCFLPDGKHFLFYASEGSGT
                     EPGVIRLGVVGSTATRFLGASHSRAVFLPPHRVFYVRGETLVTQDLDVDRGALTGDPR
                     PLGIELRGSIGISGFRYLAAAGPETLVTRRIDANATQLLWVDRKGKVLSVAVDDGGWH
                     NLPRIAPDGRRIAVAHYSAGVSHGDIHVHDAERHLDTRVTFDEDDDQEVAWSPDGRTL
                     AVTSSRTVGQTISLVDPARPGERRDLRSIEKGSTGGDWFPDGSLIVTANDASGLSDLI
                     RLPANGDPPVPVVVTPFSARDPSLTRDGRWLAYVSDSTGREEVYVRALASGEEWRVSK
                     DGGQAPVWRRDGKELFYRDTRGSILAVPTTLGATFSMGVAQELFPGFLDDASSGRQYD
                     ASPDGQRFIVNRNKVTTERPILVHLGLPEAGRTAR"
     gene            complement(8880..9158)
                     /locus_tag="VFD06_07265"
     CDS             complement(8880..9158)
                     /locus_tag="VFD06_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03373.1"
                     /translation="MQSDLHAELDAAESAFARRALRRRPLFLALSIAGIAVALGLSSY
                     YAWRRAHDPQFALAARAVIVVLVLLNARQNLRQYRYATVLGKVLPPTP"
     gene            9366..9980
                     /locus_tag="VFD06_07270"
     CDS             9366..9980
                     /locus_tag="VFD06_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZN03374.1"
                     /translation="MPLVAAVGGLVATVGAVGFVWYYFSPKFTDAGYRPVQPVAYSHK
                     LHVGDLGLDCRYCHASVEVSPVANVPPTSACMNCHRLVKRDSPLLEPIRESASSGRPM
                     RWVRVHKLPDYAYFEHRSHIAAGIGCVSCHGRIDEMDVVKQVQPLSMSWCLDCHRNPG
                     PNRRPVSEVTNMKWAPPKDASALAAKLQQERPVHPPIECSGCHR"
     gene            9977..>10426
                     /locus_tag="VFD06_07275"
     CDS             9977..>10426
                     /locus_tag="VFD06_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HZN03375.1"
                     /translation="MSREYWRSPRQQEQDPAFLEVLHREFPEGASEPPDGMSRRDILT
                     LLGASVSLAGLAACRRPVHHIVPFVEPPEQVIPGVPRYYATTMPFGRSAVGLVVESHE
                     GHPTKIEGNPRHPGSQGSSSVRAQAAILNLYDPDRGQHLLQGGQRKTW"
BASE COUNT         1542 a         3731 c         3653 g         1500 t
ORIGIN      
        1 gattcctcga tcgccgtctc gcctcgatcg gggaacacat tcgatgcggt gcccgtcgtg
       61 ctcggccatc ctctctcggc cggcgttgag gtgcgagcgg tgcggcgctc cgctgccggc
      121 atccggatgg accaccattc cgggggcgaa gcctgacctg cgcgccgcct ctctcgagtt
      181 cgcacccggc agcgacttcg ccgagcggtt cacgatcatc gagcgcaccg gcgagggggg
      241 gatgggggtc gtctacaagg cgatcgaccg ccgtctcgat cacaccgtcg ccctcaagct
      301 gatccagccc accatcgcct cgcacccgga ggccatccgt cgcttcaaga gcgaggtcct
      361 cctggcgcgg cagatcagcc acccgaacgt ctgccgcgtg cacgacctgg gggagagcac
      421 cggcatcctc tacctctcca tggcctgggt cgagggggag acactgcacc ggttcctggc
      481 gcagtccggg acgatgaccc cggcggcggc gctcggcatc gtcgagcggg tggcgcgggc
      541 gctggaggcg gcgcacgccc ggcgcgtcgt gcaccgcgac ctcaagccgg acaacatcat
      601 gatcgacgcg gggggagaga tctgcgtcat ggacttcggg ctcgccaccg tcaccggcga
      661 tgccgccaag agcggcggtc gcatcgtcgg caccccgccc tacatgtctc cggagcagcg
      721 gcgcggcgag gaggccgacc cgcgctccga cctgtacgcg ctggggatca tcctttggga
      781 gctgctggcc gggcgcctcc cctcctcccc gctggacccg gcctcggtga aatccgacgt
      841 gccgcggccg ctcgcatcca tcctggcctc gctgctcgcc gaggaccgcg gggcgcgctg
      901 cgcgtcggcg accgttctgc tcgctgcgat cgccgaggcg cgcgagcgct tccccgagct
      961 cgggaagaga tcccctatcc ctcgacggcg ccgctggcgc gcggtggcgg ccgccgccgt
     1021 cctggcgggg gcggccctgg tgtacgtggc ggtccgcccg cacctggatg cgcgccctcc
     1081 gcgcgccccc acgatcacca ccgtgcctcc ggagaccgcc gggttcttcg agcgcggcct
     1141 ctactacctg cgcgacgagg gggagacggc gcgcggcctg ggggatgcca tccagatgct
     1201 gcaccgggcg gtcgggaaga cccccgagga cggccgcgcc tgggcggtgc tgggtgaggc
     1261 gtactgggtc cgcttccgcc agacgggcgc ccccgaggcg aaagcggagg ccgagcgagc
     1321 gctagcgcgg gccacgacac tcgcccccca tcttcccgag acgctgcacg cccgaggcct
     1381 cggaacgctg gtcgcggggg acgcgggcgc cgcccgcgcc gacttcgaga aggccaccca
     1441 gctcgccccc gacttcgaca gcgcctggga gagcctcggc aacgccgacc gcgacctcgg
     1501 caactacgcc gaggggctgc gcgagctgca gacggcggtg cgcctccgac cgaccagcta
     1561 cctgcaccag atcgccctcg gccgcttctt ccagcgcttc gccgaatacg acgccgccgc
     1621 cgcggcctat cgcaaggcga tcgacctgaa gccggaccag ccgtggggct ggaacaacct
     1681 cggcgcgatg tacctgttcc gggcgcgccg ccccgaggag gcggtccccg ccttcaagcg
     1741 ctccatcgct ctcgaggatc gcggcatcgc ccgcaccaac ctcgggaccg cctactttta
     1801 catggggaac tacgaggagg cgctggcgca gtaccgccgc gccaccgagc tggaatccga
     1861 ggacgccgac aactggggga acgtcggcga cgccctctac atgctgcgcc gccccgagga
     1921 ggcgcgccgc gcctaccagc ggggcgtggc gctggcgcag gcaggggtga cggcgcgccc
     1981 gctcgacgcc ggctcccgca accagctcgc caagtactgc gcgaaggcgg gccgcgccga
     2041 ctgcgctctc gaggaggcgc gccgcgccgc cgagatgcag ccggacaacc ccgacatgga
     2101 cttcaccaac gccctggcgt acgccgcttc cgggaagctg accgaggcgc tcgactggct
     2161 cgagaaaggg gtgaagcacg gcctcggccg cgcgcagatc gaatacgagc cgttcttcgc
     2221 gccgctgcac gcctcggcgc gctaccgtgc gattctcgcc caggcggtct gacggacgct
     2281 aggagcctgt cagggcccgt cggtccggat cgtcggatcg agcgtcgccc attcgagctt
     2341 gttgtcgtac tcgtcgaacg cctcgatcgt gtacttgtcc ggccgcgact gtggcccgcc
     2401 gctggtgaag tagttcggcg cctgccccca gatcgtgtgc cggtgggcga cgatcttctt
     2461 catctggttg gtcggcggca gcgccggtct cgggaagaag gtcgccgtcg attcctcgaa
     2521 ggagatgcgc accgcccgca ccggcgtgcc ggcggtatgg aagtgccagt tcaggatctc
     2581 gttctgcatc accgattccg gatcctggag caccatcgcc ggcattcctg tcttgaagac
     2641 cacctgaatg tccgccatac cgacctcccg ggccccggtt acggaccccc ctgagcttag
     2701 ctgtgggctg gaaaagctgt tgtgacgtta cgcgccgtcg tacgcgagtg tcaagcgggc
     2761 atggtgccac tccaacccgc cccgcgccgg cctggaggga gcccggatgg cgcggggcgg
     2821 gcccggccgc agcccggatg aatcgcgacc gatggcggca cgggcgcgag tggctcgccc
     2881 ccccaccgag ggatgcgggc gccgcacccg ctgtcctccc gcacccgtgc atctctggat
     2941 gtcataccgc cgatgactga tctgccgttc ttcgctgtct gtgtcgcagc tgggggggaa
     3001 gcgttagcgg actttctcgt tcctgctcgg tacaccgccg gcgcatttgg actaatgtcg
     3061 cgccggagcg aggagcgtta gcgattttca gctcagtcca tgttggacgg accattcggc
     3121 tgcttccgcc gcataacatg agggtggggg tcggcacgac gatgtccgac atcgacggca
     3181 aatccgggga atccgggccc gatcgcgtcc tgcgatacct tgacgggaat gcctacgtct
     3241 acgcgcggac ccaggcggcc ctgatcgccc gctgcggcgg gtacgacatt ccctcgtcgg
     3301 agcaggcgga catcgtccag acggtgctct acgaggcctg ccaggcggtc gcccgaccgg
     3361 atttcaaact gcgcaaggag ttcggccact tcatccgcac cctggcgcac cgccgctgca
     3421 tcgactggtg gcgccggaac cgccccaatg tcccgatgga gctcgaccgg ccgagccttt
     3481 cccccctccc cgacgagaaa atactcgcaa acgaacgtct cgcactggcg acagagattg
     3541 tgaatcagct gcccccgacg tgccgggagc tgatcgagaa gcacatcttc gcgggcctcc
     3601 cctacgccga gatcgcggcc accacgggga ggacggaagg tgcgctgcgc gtggagatgt
     3661 gcaagtgcct cgagaaggcc cggcagctcg cccgacggct cctcctgcag ccgggcagtc
     3721 gcggcggggg gcagggcgac ctccgatgac ccacgatgag gcggtcctgc tgctgacgga
     3781 tctgctctcg gggcggctcg ccccgcgggt gcgccaggat gtgcaggggc acctcgaggg
     3841 gtgctccgag tgcgcctcgc tcgtggggac gcacgagatg ctcgcgggcc ggcacacgcc
     3901 ggtcgaggat ctcgtcgatc tgtcgctgca gccggatcgg atcgagaacg cgcgacgcat
     3961 gcgcctgcag gcgcacctcg acgtctgcga tgcctgcggc gcggaggcgc gcctggtgct
     4021 caaggccgag cgcgaggcgg cgaccgacgc caccggggac ccgcgtggga gcgggagcct
     4081 gctcctgggc gcggccgcga cggcgctgct cgggctcggt ctgtcggcct acctcgggct
     4141 gtatcggatc ccccgcctcg aatcgacgat cgatcggctg gaagcggtgc cgccgaggcg
     4201 gatcgagtcc gcgccgcagc cgcacgagcc gggaccgatg gtgcggatcc agtaccttcc
     4261 gtcggcgcgc cgggggccgg gttccgacgc catggtctcg ctcgaccccg ggcagccgta
     4321 ccagtacctc gccgtcgcgg tgccggagtt cgagccgcgc gggacctata ccgtggacct
     4381 gaccgacccg gaagggcacc aggtctggtc gcgcacggtc ccggcggagg agctgcgccg
     4441 cctcacgaag gggacgccgg agatcacgct cgcggtcccg tcgcgggcgc tccatgaggg
     4501 gcgattcgtc ctgcggctcc gaggcccctc ggggcccacc gcggggccca ccgcggggcc
     4561 gctgctcgag accgccttcg aggtccgacc ggcgccctag taagtcgccg gtctcacttc
     4621 ccccgcagga acgcctcgat ccgcttcacc caggcgtcgc tctcctcgca gaaagggagg
     4681 tggccgcttt tctcgaagac ggcgaactcc gagcccggga ccttctggtg gatggcatac
     4741 gcgaccgagg gcgcgacgtt gaagtcgtag cggccggtgg ccaccagcgt gggaaaccgg
     4801 tacttgagca gctcggggtt gagatcgtag cgctccgctt cgccccacaa cgcctggttg
     4861 atcttcggac ggaacacgaa cgaatccgat cgcagaaggt aggcgtcccg cacctcgttc
     4921 gagacgaaca acatcgacga gtactcgcgc aggctccggg cgatcgcctc cggatcgccg
     4981 agcgcctcgg cgaaggcgag cgcgtgctgg cgctccgtcg tctccgggta gatgtgcttg
     5041 aacaggaacg ccgtctcctg gatcctcggg gcggcggagt cggcgatgac caggtgctgg
     5101 atgcgctccg ggtggcgcgc cgcgtaggcc atcacgaggt atccccccca ggagtggccg
     5161 aggaggtcga acttctgggc tccgaggtgc gtccgcagcg cgtcgaggtc ggcgatctgg
     5221 tcgaggagct tcgtcgtctg gtcgtccttg agcggcgtcg agcggccgtt gccgcgctgg
     5281 tcgtagaaga cgacgcggcg gccggccgcc agacgatccc aggcatcgga gcagtgcagg
     5341 taggcgtggt cgaagccggg gccgccgttg acgacgaaga gcggcgtccc gctcccctgc
     5401 cccttcacct cgtagaagat cgacaggccg ggggcgatct ccagcatctc ccctttctcc
     5461 ggagcgggcg ccttgatggc gggagccgcg gcggccgtca ggcaggctgc cgcgagcagg
     5521 atcgaaagac gcacgacgga catcgttctc ctcctcatct cacttttccg tcaggatcgg
     5581 cgctcctcac gtgctgggcg cgattgtaga tttcgttgaa gccgtcctgg tcggcgccgt
     5641 accagccgcg caaccctccg gtggcgtcga ccaggagcag cgttcccagg cgcgacagtc
     5701 cgccgaaggc ctgcagatcc ttctcgtcga gacgcgccgc gatccagagc ggcggggcgg
     5761 gatcggcggg cccggccggc gggggtgccg gcggcgcctc catgggctgg tccggatcgg
     5821 cctcgacgga atccatatgg tccggctccg gctcggccgg ccgggcggtg tcgacctcga
     5881 ggctcaggag ccggatccct ccgagatgga aaccggcgag ggcgtccttc agctccgcca
     5941 tccggccgcg ccgctcggcg caggcctcga ggcacgggac actctcgatc gacaccaccc
     6001 acgcggagcc cttgagggat gccggatcga tggccgctcc atccgcggcg atcagacgca
     6061 ccgcggggaa gctcccctgg accggcggcg gcgcggggac gcgacgcagg caggggcgca
     6121 ggagggtcag gacgacgatg ccgaggagaa aggcccacag aaacgtgaag cgcgggagtc
     6181 ggcgagcgcg gccgtccggg gcgtcggtca ccgggccgtc cggccggcct ccggcaggcc
     6241 gaggtgcacg aggatcggtc gctccgtcgt caccttgttg cgattgacga tgaaccgctg
     6301 accgtccggg gacgcgtcgt actggcggcc gctggacgca tcgtcgagga aaccggggaa
     6361 cagctcctga gcgaccccca tggagaaggt cgcgccgagc gtcgtcggga ccgccaggat
     6421 cgagcctctc gtgtcgcgat agaagagctc tttcccgtcg cgtctccaca ccggcgcctg
     6481 ccctccgtcc ttcgagacgc gccattcctc gccgctcgcg agcgcgcgca cgtagacctc
     6541 ctcgcggccg gtggagtcgg agacatacgc gagccagcgg ccgtcgcggg tcagggacgg
     6601 atcccttgcc gagaaggggg tcacgacgac cggcaccggc gggtccccgt tcgccgggag
     6661 acggatgaga tcggagaggc cggaggcatc gttggcggtc acgatcagcg acccgtccgg
     6721 gaaccagtct ccacccgtcg accccttttc gatcgatcgc aggtcccggc gctcccccgg
     6781 acgcgccggg tcgacgagcg agatcgtctg ccccaccgtg cggctggagg tgaccgcgag
     6841 cgtgcggccg tccggcgacc aggcgacctc ctgatcgtca tcctcgtcga acgtgacccg
     6901 ggtgtcgaga tgccgctccg cgtcgtggac gtggatgtcg ccgtggctga ctcccgccga
     6961 gtagtgcgcg acggcgattc tcctgccgtc gggagcgatg cgcggcaggt tgtgccagcc
     7021 tccgtcgtcg accgccaccg acagcacctt ccccttgcga tcgacccaga ggagctgggt
     7081 ggcgttcgcg tcgatcctcc gggtgacgag cgtctccggt cccgcggcgg ccaggtagcg
     7141 gaagccgctg atgccgatgc tgccgcgcag ctcgatgccg agcggccgcg gatctcccgt
     7201 gagcgcgccg cgatcgacgt cgaggtcctg ggtcacgagc gtctcgcccc gcacgtagaa
     7261 cacccggtgc ggcggcagga agaccgcccg cgagtgggac gctcccagaa aacgggtcgc
     7321 cgtcgatccc acgaccccca gccggatcac tcccggctcg gtcccggaac cttccgaggc
     7381 gtagaagaga aaatgcttcc cgtccggcag aaagcagggc cagcggtgcg tcccctcctt
     7441 cccttcgagc accgtcgccg ggctcgcggt tcccccgccg gcggacatcg accggaggcc
     7501 tccgtagaac gtcggcgcga agatgatgac gtcgccggcg ccccacgtgg cgccgcgccc
     7561 gtccgaagtg ttcgtcgtcc gcacgaggct gcgcacggcg ccgccgacga gatccaccga
     7621 tttcagctgg ctgccggcaa agaagccgat ctgccggccg tccggcgccc agaacgggaa
     7681 gcgggcccct tccgtcccgg cgatgcggcg cggctcgacc tcgccgagat cacggaccca
     7741 gagggcggtc tggccggccg gatcgcgcgc gacgaaggcc agggatcgcg cgtcacgcga
     7801 caggtcgacg ccaccgatga acgtgtgccc ggcgggagga tcgaagccga actccagccg
     7861 ctgcgtcgag gacggcggcg gctcccggcg cggggcgagc aacagcgtcg ccgcgacccc
     7921 cgccccggcc gccaggagcg cccatccgac cgcggtccgt cgcgagatcc ccgcacgcga
     7981 cgtcgccggc ccggcgggcg cggcggccgc gtccggcacc ccggagcccg gttcgagcgc
     8041 cagccgcgcc tcgccgatcg cctgcagccg gcggcgcgga tcccgctcca ggcagcggcg
     8101 caggagcgcc gcgacccgcg gcggagtgtc cgggggcagc gccgtccagt ccggctcgcg
     8161 cgtcagcacg gcggccagga cgtcggtcag ggtctccccg gtgaagagcg gccgcccggc
     8221 gagcatctcc cacagcacgg cgccgaacgc ccagatgtcg gcccgccgat cgacggcgcg
     8281 gccgcgcgcc tgctccggcg ccatgtacgc ggcggtgccg aggatcattc cggcccgcgt
     8341 gccgctctgc acgagcgtcg gcgagcggga caggtccgat ccggccacgg gcgcctccgc
     8401 cgccgtgaag gccttcgcca gcccgaagtc gagcaccttg accttgccgt ccggcgtcac
     8461 cttcacgttc gccggcttga ggtccctgtg gacgattccc ttctcgtgcg cctcctcgag
     8521 tgcctcggag atcctccggg cgatctcgat cgcctcctca tgcgggatcg ggccgcgcgc
     8581 caggcgctgc gccaggtcct cgccctccac cagctcgagc accaggcaag ggatgcctcc
     8641 cgcgtcctcg aggccatgga tggcggcgat gttcggatgg ttcagcgacg cgagcacctg
     8701 cgcctcgcgg cggaagcggc cgaggcgatc ggggtcggcg ccgacatcgg ccggcaggat
     8761 cttgagggcg acctctcgtc ccagccgtgg gtcgacggcg cggtacacct ctcccatgcc
     8821 gccgctgccg atgagcccgc ggatctcgta cggaccgacg cggctcccgg ggaggagggt
     8881 cacggagtgg gcggcaggac cttgccgagc accgtggcgt agcggtactg gcgcaggttc
     8941 tgccgtgcgt tcaggaggac gagcaccacg atgacggcgc gcgccgcgag ggcgaattgc
     9001 ggatcgtgcg cccgtcgcca ggcgtagtag ctcgaaagcc ccagcgcgac ggcgattccc
     9061 gcgatcgaca gcgccaaaaa caacggccgg cggcgcagcg cgcggcgcgc gaaggcggac
     9121 tcggcggcgt ccagttctgc gtggagatcg gactgcatcg aatcgcggat tatagagtga
     9181 aaaacggcct gcgcgggccg cttccaaggt cttgaccctc acgagagcgt gagactagta
     9241 tcgccggtcc tgaatcccgg gaggagacgt ttccgtgccc cagatcggtc gacgccgcga
     9301 cgcgcgacgc caggagatcc gaccgtagcg caatggcgca gattctccct ccttggatca
     9361 accgattgcc gctcgtcgct gccgtgggcg gtctggttgc aaccgtcgga gccgtcggct
     9421 tcgtctggta ctacttttct ccgaagttca ccgacgccgg ctaccgtccg gtgcagccgg
     9481 tcgcgtacag ccacaagctg cacgtcggcg atctcggcct cgactgccgc tactgccacg
     9541 cctcggtgga ggtctcgccg gtcgccaacg tgccgccgac ctccgcctgc atgaactgcc
     9601 accgcctggt gaagcgtgac agcccgctcc tcgagccgat ccgcgagagc gcctcatcgg
     9661 gccggccgat gcgctgggtg cgggtgcaca agctgccgga ctacgcctac ttcgagcacc
     9721 ggtcgcacat cgcggccggc atcggctgcg tctcctgcca cggacgcatc gacgagatgg
     9781 acgtcgtgaa gcaggtgcag ccgctgtcga tgtcgtggtg cctggactgt caccgcaatc
     9841 ccgggccgaa ccggaggcca gtgagcgagg tcaccaacat gaagtgggct cccccgaagg
     9901 acgcgagcgc tctcgccgcg aagctgcagc aggagcgccc ggtccacccc ccgatcgaat
     9961 gttccgggtg ccaccgatga gccgcgagta ctggcggagc cccaggcagc aggagcagga
    10021 cccggcgttc ctcgaggtcc tgcaccgcga gttcccggag ggggcgagcg agccgcccga
    10081 cgggatgtcg cggcgcgaca tcctgaccct cctcggggcg tcggtgtcgc tcgcggggct
    10141 cgccgcgtgc cgccgccccg ttcaccacat cgtgccgttc gtcgagcctc ccgagcaggt
    10201 gatcccgggc gtgccgcgct actacgccac gacgatgccg ttcggccgca gcgccgtcgg
    10261 cctggtggtc gagagccacg aggggcatcc gacgaagatc gaggggaacc cgcgccaccc
    10321 cggaagccag gggtcgtcca gcgtccgggc gcaggcggcg atcctcaacc tctacgatcc
    10381 ggaccgcggg cagcatctcc tgcagggcgg ccagcgaaag acgtgg
//
LOCUS       DASRIQ010000300         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1685408, whole genome shotgun sequence.
ACCESSION   DASRIQ010000300 DASRIQ010000000
VERSION     DASRIQ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685408"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1143)
                     /locus_tag="VFD06_07280"
     CDS             complement(157..1143)
                     /locus_tag="VFD06_07280"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZN03376.1"
                     /translation="MSPETATSLRTKLIDRRRQLEKAVAERGSLDDIVRLVEQVDAAL
                     RRMDTSTYGTCSVCGEGIEESTLLTNPFAEYCLCRLNDQQQAALQLDLDLAQRIQRGL
                     LPPQDVRTAGWEAHYRYQPAGPVSGDYCDLLTTGNGDGDLYFAVADVSGKGVAAALLM
                     AHLNASFRSLIQVGLPLPEILARQNRLLLDSQLPSQYATLVCGRARRDGIVEICNAGH
                     WPPLVTRRDRIEALEESGLPIGMIGDRRYETASVRLEPGETLFLYTDGLVEARDGGDA
                     EFGAARLGELLARHRALAPRALADACLETLAEFLHGSRPSDDLSLMAIRREL"
     gene            complement(1189..1950)
                     /locus_tag="VFD06_07285"
     CDS             complement(1189..1950)
                     /locus_tag="VFD06_07285"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HZN03377.1"
                     /translation="MTDQPPSPSDVPPAPPWAPAPPSAPETFAPPPPIATPSIPSEPL
                     NPWVSLWTRPRATMRQILDTNPRRWVHVLAILGGIAEFIITPIPDTPPFPQLSPAAML
                     AFKCVSGAIGGLIGLYIASFLGWMTGRWLGGQGNLVEVRSASAWPNILTLWSTLLWLP
                     LVVYLGMEAVNFNPEALSEEPTGMVLFATIRILLVVAAIWRLVVYLKCLAEAHRFSAW
                     HALGASLIGVVLIGVVVAILVGIVIGIVGLSAFSS"
BASE COUNT          365 a          686 c          779 g          280 t
ORIGIN      
        1 atgacgcccg gcgacgagga gcagtggacc atctcggtgc agcacaccga ggcggacatc
       61 gacgcccacc tcaaggcgtt cggcagggtg gcgccgctcc tcaagaaact agacgcataa
      121 aggtcccgcg agggcgccgc agaaacacga gggggctcag agctcgcggc ggatcgccat
      181 cagcgacagg tcgtccgagg ggcgcgagcc gtgcaggaac tccgcgaggg tctccaggca
      241 cgcgtccgcc agggcccgcg gggcgagcgc gcggtgccgg gccaggagct cgccaaggcg
      301 cgccgcgccg aactcggcgt cgccgccgtc gcgcgcctcg accaggccgt cggtgtagag
      361 gaacaacgtc tctcccggct ccagccggac gctcgcggtc tcgtagcggc gatcgccgat
      421 catcccgatc ggcagccccg actcctccag cgcctcgatc cggtcccgcc gcgtcacgag
      481 cggcggccag tggccggcgt tgcagatctc gacgatcccg tcgcgccgcg cgcggccgca
      541 gacgagggtc gcgtactgcg acggcagctg gctgtcgagc agcaggcggt tctgccgcgc
      601 caggatctcg gggaggggca ggcccacctg gatgaggctg cggaaggagg cgttcaggtg
      661 cgccatgagg agcgccgccg ccaccccctt cccggagaca tcggccaccg cgaagtacag
      721 gtcgccgtcc ccgttgccgg tcgtgagcag atcgcaatag tcgccgctca cgggtcccgc
      781 cggctggtag cggtaatgcg cctcccagcc cgccgtccgc acgtcctgcg gcggaaggag
      841 cccgcgctgg atccgctgcg ccaggtcgag gtcgagctgc agcgccgcct gctgctggtc
      901 gttcaggcgg cacaggcagt actcggcgaa gggattggtc agaagcgtcg attcctcgat
      961 cccctcgccg cagacggagc aggtgccgta ggtgctggtg tccatgcggc gcagggccgc
     1021 gtccacctgc tccacgaggc ggacgatgtc atcgaggctg ccgcgctccg cgaccgcctt
     1081 ctcgagctgc cggcgtcgat cgatgagctt ggtccggagg gatgtggcgg tttcggggct
     1141 catgggaaag gacctgcccc gattatagaa gaaaaagcga aattatgttc aggaagaaaa
     1201 cgccgagagc ccgacgatgc cgatcacgat gccgaccagg atggcgacca ccacgccgat
     1261 caggacgacc ccgatgagcg acgctccgag cgcatgccag gcggagaagc ggtgcgcctc
     1321 ggcgagacac ttgaggtaga cgacgagacg ccagatggcc gccacgacga gcaggatgcg
     1381 gatcgtggcg aacagcacca ttccggtcgg ttcctcggac agcgcctccg ggttgaagtt
     1441 caccgcctcc atccccaggt agacgaccag gggcagccac aacagtgtgg accagagggt
     1501 caggatgttg ggccaggcgg aggcggagcg cacctcgacg agattcccct ggccgccgag
     1561 ccagcgtccg gtcatccatc ccaggaagga ggcgatgtag agcccgatga gtccgccgat
     1621 cgctcccgac acgcacttga acgccagcat cgccgcgggg gacagctgtg ggaacggcgg
     1681 ggtgtcgggg atgggcgtga tgatgaactc ggcaatccct ccgaggatcg cgagcacgtg
     1741 gacccagcgg cgcggattcg tgtcgaggat ctggcgcatc gtggcgcgcg gccgcgtcca
     1801 cagcgacacc catgggttga gcggctcgga cgggatcgac ggcgtcgcga tcgggggagg
     1861 aggcgcgaag gtctcgggcg cggacggagg cgccggcgcc cagggcgggg cgggaggaac
     1921 gtcgctgggg gaggggggct ggtcggtcat cggatcggaa tcatggacgc gtcaccggag
     1981 atccgcggaa gagccgaccg cgcctcccgc ccgcgacggg gcgattccgc gtaccgcggg
     2041 cgggagggtg aggtgcgaag aacgccgtcg tccgatcagg ccttgaggtg cttgttgacg
     2101 agcttggtca
//
LOCUS       DASRIQ010000301        10150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2453674, whole genome shotgun sequence.
ACCESSION   DASRIQ010000301 DASRIQ010000000
VERSION     DASRIQ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2453674"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFD06_07290"
     CDS             <1..548
                     /locus_tag="VFD06_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN03378.1"
                     /translation="INTITLRVPPLRERSEDIPLLARHFARDIAARLGRRAPALTESA
                     ASLLKTYHWPGNARELRNVVERAMILSPGETLSGFDLPPAGGPVPAAAGVAAPARAAV
                     QVADAPSSSGAPTGSETTPAAEAMALRTVIAESERRAVIEALRRARGVRKEAARLLGI
                     DQRNLAYYFRKHGIDPDALPD"
     gene            532..3486
                     /locus_tag="VFD06_07295"
     CDS             532..3486
                     /locus_tag="VFD06_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03379.1"
                     /translation="MPSRIEPGPNRLAARLFALALAFEVANAAYLAAFDSATIFYHAM
                     VVAHVVAGLFLVPLGLALCARWLRGLLREWGAGMRVAAAASALVAIGTALPLAVVGTA
                     TPYRSLLTTHIVSACLALLFAVLAALMAGPAAVVRARRTALFLGVALAFALAVRGAGW
                     IWPAHVARIENPITPPLTPFDEGGGKNNQFFPSSVTTVGDRTVPDDFFLESKACGNKG
                     CHPDITKQWDSSMHHFSSFNNQWYRKSIEYMQEVVGTEPSKWCGGCHDQSVLLTGRMG
                     RPIVEQIDTPQAQAGIGCLVCHSIVHVKDTMGQGGYVLEYPEMHRLVASQNRALHAMH
                     DYMVRLDPGPHKKTMLKPFHRESRAEFCSSCHKVHLDVPVNSYRWFRGFNEYDAWQGS
                     GVSGQGARAFYYPDKPKDCADCHMPLVKSADAGNRDGFVHSHRFPGANTAVPFANKDE
                     EQLRITQEFLRANILSVDVFAVTESAAGSAGEAAPAPGPDEPVAQTMFPDIEAAMTAP
                     RTGLGAAARRAPVVAPLDRSAPFVRPGGTYRVDVVARTRGVGHFFPGGTVDAFDVWLE
                     LKGEDAGGRVFFWNGYLEDGGRGPVEAGAHRYRSLMVDAHGNPINKRNAWATRALVYA
                     RLIPPGAADVGRFRVEVPRDAQGPLTFTARMNYRKFSWWNTQFSFAGVRDPAVPDVQV
                     TPHFDDGPFVFTGDTSRVSGQIRGIPDLPVTVVAEDKVTVELVPGAGAAAPARAADLA
                     AAIPAGQAFDRERWNDYGIGLLLQGDLVGAKAAFETVTRLEPGDAAGFVNLGRVLVQE
                     GDHEAAMKVLEKALEVDPNIASGHYFLALSLKSRGRYDEALDHLRQAAARFPRDRVVR
                     NQIGRILFLKRQHREAIAEFEQTLKVDPEDLTAHYNLMLCYRALGEADAERREETLYT
                     RFKADESAQALTGAYRRLNPEDNIERQPIHEHVSRWRPSAVATAPDPAGKPQAAGTQA
                     AVGNPPVVQGGR"
     gene            3483..5201
                     /locus_tag="VFD06_07300"
     CDS             3483..5201
                     /locus_tag="VFD06_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZN03380.1"
                     /translation="MRAVLRAMGAALLALGMAGGLACNSKPKAEAPKPAPPEKANPVV
                     FTDVTKAAGIAFTHNNGARGKKYLPETMGSGLIFFDYDGDGWQDLFFVNSADWPESRR
                     GPSWQALYRNRGDGTFEDTTKKAGLLVESYGIGATAADYDNDGDTDLFLNALGPDHLW
                     RNRGDGTFEEVTKAAGVGDPGFGSSATWLDYDRDGFLDLFVCNYVQWSPKSDIYCTLD
                     GANKSYCTPESYSGATNRLFRNRGDGTFEDATQKAGVHDPTGKSLGVVALDYDDDGFM
                     DLAVANDTQPNFLYRNRGNGTFEEVGKSAGIAYSEEGKARGAMGIDAADYDDSGRDSL
                     VIGNFSNEMLALYHNEGKGLFIDDAAAAGIGQPSLLTLAFGCFFFDYDLDGRLDIFVA
                     NGHVENEINRVQPSVTYAEPPHLFRGLPGGRFEETTASVGPDLARPVVARGAAYADYD
                     QDGDLDVAYSANGGPAVLLRNDGGGAGGWMRLRLVGSPSNRDAIGARVRLTSGGRTQT
                     RTVRAAASYASQSPMVLTFGLGGSGKGDAATAEVRWPDGSKQAFTGLAPGKTMVLKQG
                     SPTATD"
     gene            5423..8899
                     /locus_tag="VFD06_07305"
     CDS             5423..8899
                     /locus_tag="VFD06_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZN03381.1"
                     /translation="MARAGTGLFEHPVFPTGEMPRRVAIGDFNGDGVPDVVTVNAASD
                     DVSVLLGAGADGLMPERRYPAGDLPYGLTLGEFDGDGLLDLAVTNCDSRDVFVYIGRG
                     DGRFAVTDRLPAGGCPTSILASDLDADGLQDLALVNDFSNELWIYPGLGGARFGPAAV
                     SPLDRWPILGGAADFDRDGHMDLLVSAAAERSVTVLFGDGALAFPRRAVVPIAGWVGA
                     LVIHDFDGDGSPDFAAAHFPAAPGGAALQVFRGQGDGTFTAVQDLPPGYEPLVLAAAD
                     LTGDGAADLVALNGYPAVLVVFVGHGDATFQSGVVFGTGPDPGGLGLADIDRDGRTDA
                     LATSTSGHAVWMHRGLGDGGLETTRLFAAGDGPDRVVSADFDQDGAADLAVASAYGGI
                     DGKGHITTLQGDGVGGFTPSHDLAIAGNADDLATGDLNGDGFPDLAAAAVGDRWGSPP
                     VPSGASILLGDGAGGFTPEILIEDPDDEPRGIAIGLVNHDETPDIVLSGSLVSTWPGR
                     GDGTFAAPVRSEVGTWPSRAVLADLNGDGTTDLAVANGNPAVICPLWPYPCPPPKPGD
                     ISVLLGLPGGGFAPEVRYAAGMFPSSIAAGDLDDDGAMDLVANGDGDLSIFMGQGDGT
                     LAARRRVAAGSTPGAILINDFDGDGVLDVVTADAGGNTVSVLPGRGDGTFLASTQYQA
                     GVLPFDCASGDFNGDGRLDLATTSYWPNGAVVLLNQGPPDADGDGVIDREDPCTDLDG
                     DGAGDPVLPANTCAADNCPSLPNPAQEDADHDGVGDACDTCPRASDMEQQDTDRDGVG
                     DVCDDCPDTPDPLQSAASDADSIGDACDNCPTVVNPMQEDADGDGTGDACDLCSDADG
                     DGWGDARFPASTCAADNCPATSNPGQEDGDTDGLGDGCDNCPAASNAGQADRDGDGAG
                     DACQPALDLRGVRPHGRTSLVALLSASDPQGEPLPGTLRIERLPFTLVLSDAGPIPSC
                     NEGFLPEDQAGEGVGFLYGDRSGPVLYDLDSNLGCDDGLPDFGIAVGPCDAPLTMFFE
                     LLELNAWSNTIPETLCVRAVGDPGGGLELTVEGLEPDRLVTTARDTTPAVSIEFQNGP
                     PDSLDILSLSAGADYRLAVTITDGNTPPVTAQADFRHRRETVMLIGLPPAIGMTARPV
                     VPARPTRPTPAS"
     gene            9065..>10150
                     /locus_tag="VFD06_07310"
     CDS             9065..>10150
                     /locus_tag="VFD06_07310"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HZN03382.1"
                     /translation="MKFVAGNVALLFFYYAAGRLGLALAYLHPSASPVWPPSGIALAA
                     LLVLGYQSWPTIFLGSFLVNVATAGKVAMSLGIATGNTLEALFAAYLVNHYASGVKAF
                     YRPEDFLRFGGLAALLSTAVSATLGFASLALGGAVRWDQVASVWTTWWLGDAVGILLV
                     TPFLVLWAARPQTPWRGRELEVILLMLCTASVGWVVFSDALPLRSGSLPLGYLCILPL
                     LWAALRFGQRTVATGVLVLSAVALWETVAALGGVGGAARAAVNERLLLLQLFLGSVSA
                     MAVGVGAWVSQGRRAEEELLRSREWLERKVMERTLEISSASAGLQQSEARFRELLESA
                     PDAMVLVDRSGTIVLVNAQTEKMFGRSR"
BASE COUNT         1472 a         3620 c         3525 g         1533 t
ORIGIN      
        1 gcatcaacac catcacgctg cgcgtgccgc cgctgcgcga gcggagcgag gacattccgc
       61 tcctggcgcg ccatttcgcc cgcgacatcg cggcgcgcct cgggcgccgc gcgccggccc
      121 tgacggagag cgccgcctcg ctgctgaaga cgtatcactg gccggggaac gcccgcgagc
      181 tgcgcaacgt cgtcgagcgg gcgatgatcc tgagcccggg cgagacgctg tcggggttcg
      241 acctgccgcc ggccggaggg ccggtcccgg ccgccgccgg cgtcgcggcg ccggccaggg
      301 cggcggtcca ggtcgcagat gcaccctcct catcgggcgc tccgaccggc tccgagacga
      361 caccggcggc ggaggcgatg gcgctccgga cggtcatcgc cgagagcgag cgtcgggcgg
      421 tcatcgaggc cctgcgtcgg gcgcgcggcg tccgcaagga agccgcgcgg ctcctcggca
      481 tcgaccagcg caacctcgcc tactacttcc gcaagcacgg gatcgatccg gatgccctcc
      541 cggattgagc cgggcccgaa ccggctcgcg gcgcgcctgt tcgcgctggc gctggcgttc
      601 gaggtggcca acgccgcgta cctcgcggcg ttcgactcgg cgaccatctt ctatcacgcc
      661 atggtcgtgg cccacgtcgt cgcgggtctg ttcctcgtgc ccctcggcct cgcgctctgc
      721 gcccgctggc tgcgcggact tctgcgcgag tggggagcgg gcatgcgcgt cgcggccgcc
      781 gcctcggccc tggtcgccat cgggaccgcg ttgccgctcg cggtggtcgg gaccgcgacg
      841 ccgtaccggt cgctcctgac gacgcacatc gtttccgcct gcctggcgct cctcttcgcc
      901 gttctcgccg ccctcatggc cggcccggcc gcggtggtcc gggcgcgtcg caccgcgctc
      961 ttcctcggcg tggctcttgc cttcgccctc gcggtgcgcg gcgccggctg gatctggccg
     1021 gcgcacgtgg cgcgcatcga gaacccgatc accccgccgc tcacgccctt cgacgaaggg
     1081 ggcgggaaga acaaccagtt cttcccgtcg tcggtcacga ccgtcggtga ccgcaccgtg
     1141 ccggacgatt tcttcctcga gtcgaaggcg tgcggcaaca aggggtgcca cccggacatc
     1201 acgaagcaat gggactcgtc gatgcaccac ttctcctcgt tcaacaacca gtggtaccgc
     1261 aagtcgatcg agtacatgca ggaagtcgtc ggcaccgaac cgtcgaagtg gtgcggcggc
     1321 tgccatgacc agtcggtgct gctgaccggg cggatgggcc ggccgatcgt cgagcagatc
     1381 gacacgccgc aggcgcaggc cggcatcggc tgcctcgtct gccactcgat cgtgcacgtg
     1441 aaggacacga tgggacaggg ggggtacgtc ctcgagtacc cggaaatgca ccgcctcgtg
     1501 gcatcgcaga accgggcgct gcacgccatg cacgactaca tggtgcggct cgatccggga
     1561 ccccacaaga agacgatgct gaagccgttc caccgcgagt cccgcgccga gttctgctcc
     1621 tcctgccaca aggtgcacct cgacgtgccg gtcaacagct accgctggtt ccgcggcttc
     1681 aacgagtacg acgcctggca ggggagcggt gtctccgggc agggggcgcg cgctttctac
     1741 tacccggaca agccgaagga ttgcgccgac tgccacatgc cgctcgtgaa gtcggccgac
     1801 gccggcaacc gggacggctt cgtgcactcg caccgcttcc cgggcgccaa tacggccgtg
     1861 ccgttcgcca acaaggacga agagcagctg cgcatcaccc aggagttcct gcgcgccaac
     1921 atcctgagcg tcgatgtctt cgcggtgacc gagtcggccg ccgggagcgc cggcgaggcg
     1981 gcacctgcgc ccggccccga cgagccggtg gcgcagacga tgttccccga catcgaagcc
     2041 gcgatgacgg caccacgcac ggggctcggt gcggccgcac ggcgcgctcc ggtcgtcgca
     2101 ccgctcgacc ggtcggcgcc gttcgtgcgc cccggcggca cctaccgcgt ggatgtcgtc
     2161 gcgcgcacgc gcggcgtcgg gcacttcttc cccggcggca cggtggacgc cttcgacgtc
     2221 tggctcgagc tgaaggggga ggacgccggc gggcgcgtct tcttctggaa cggatatctc
     2281 gaggacggcg ggcgcggccc ggtcgaagcc ggcgcccacc gctaccgctc gctcatggtc
     2341 gacgcgcacg gcaacccgat caacaagcgc aacgcctggg cgacccgcgc gctcgtttac
     2401 gcgcgcctga tcccgcccgg ggcggccgac gtgggacgct tccgggtcga ggtgccgcgc
     2461 gacgcgcagg gcccgctgac cttcacggcc cggatgaact accggaaatt ttcctggtgg
     2521 aacacgcagt tctcgttcgc cggcgtgcgc gacccggccg ttcccgatgt ccaggtgacg
     2581 ccgcacttcg acgacggtcc cttcgtcttc accggcgaca cgtcgcgggt ctccgggcag
     2641 atcaggggga tccccgactt gccggtgacc gtcgtcgccg aggacaaggt cacggtcgag
     2701 ctcgtgcccg gtgcgggcgc cgcggcgccg gcccgcgcgg ccgacctcgc cgccgcgatc
     2761 ccggcggggc aggcgttcga ccgggagcgc tggaacgact acggcatcgg cctgctcctt
     2821 cagggggatc tcgtgggcgc gaaggcagcg ttcgagacgg tgacgcggct cgagccgggc
     2881 gacgcggccg gcttcgtcaa cctcggccgg gtgctggtgc aggaagggga ccacgaagcg
     2941 gcgatgaagg tcctcgaaaa agcgctcgag gtcgatccga acatcgccag cggccactac
     3001 ttcctggcgc tgtcgctcaa gtcgcgcggc cgctacgacg aggcgctcga ccacctgcgc
     3061 caggccgcgg cccgcttccc gcgcgaccgt gtggtgcgca accagatcgg ccgcatcctc
     3121 ttcctgaagc ggcagcaccg cgaggcgatc gccgagttcg agcagaccct caaggtcgat
     3181 cccgaggacc tgacggcgca ctacaacctc atgctctgct accgtgcgct cggcgaggcc
     3241 gatgccgaga ggcgcgaaga gaccctctac acgcgcttca aggcggacga atcggcccag
     3301 gccctgaccg gcgcctaccg gcgcctgaac ccggaggaca acatcgagcg ccagccgatc
     3361 cacgagcacg tcagccgctg gcgtccttcg gccgtcgcga cggcgccgga cccggccggg
     3421 aagcctcagg ctgccgggac gcaggcagcc gtcgggaacc ctccggtcgt tcaggggggc
     3481 cggtgagggc cgtcctgcgg gccatggggg cggcgcttct ggcgctgggg atggccggcg
     3541 ggctcgcctg caatagcaag ccgaaggctg aggcgccgaa accggctcca cccgagaagg
     3601 cgaacccggt cgtcttcacc gatgtcacga aggcggccgg catcgccttc acgcacaaca
     3661 acggggcgcg cgggaagaag taccttccgg agacgatggg gtcgggtctc atcttcttcg
     3721 actacgacgg cgacggctgg caggacctgt tcttcgtcaa cagcgccgac tggcccgaga
     3781 gccggcgcgg cccctcctgg caggcgctct atcgaaaccg tggggacggc accttcgagg
     3841 acaccacgaa gaaagccggg cttctggttg aatcgtacgg catcggcgcc accgcggccg
     3901 actacgacaa cgacggcgac acggacctgt tcctcaacgc cctgggcccc gaccatctct
     3961 ggcgcaaccg cggcgacggc accttcgagg aggtcacgaa agcggccggc gtcggcgacc
     4021 ccggcttcgg atcgagcgcc acctggctcg actacgaccg ggacggcttc ctcgacctgt
     4081 tcgtctgcaa ctacgtgcag tggtcgccga agagcgacat ctactgcacc ctcgacggcg
     4141 ccaacaagtc ctactgcacc cccgagtcgt acagcggcgc caccaaccgc ctgttccgca
     4201 accgcggcga cggcaccttc gaggacgcca cccagaaggc cggcgtgcac gacccgaccg
     4261 gcaagtcgct gggtgtcgtg gcgctcgact acgacgacga cgggttcatg gacctggcgg
     4321 tcgccaacga cacgcagccg aacttcctgt accgcaaccg cggcaacggc acgttcgagg
     4381 aggtcggcaa gtcggccggc atcgcctaca gcgaggaagg gaaggcgcgc ggggcgatgg
     4441 ggatcgacgc agccgactac gacgacagcg gccgcgacag cctggtgatc ggcaacttct
     4501 ccaacgagat gctcgccctc taccacaacg aggggaaggg gctcttcatc gacgacgccg
     4561 ccgccgccgg catcggccag cccagcctgc tgaccctggc cttcgggtgc ttcttcttcg
     4621 actacgacct cgacggccgt ctggacatct tcgtcgccaa cggacacgtc gagaacgaga
     4681 tcaaccgcgt tcaaccttcc gtcacctacg ccgagccgcc gcatctcttc cgcggcctcc
     4741 ccggcggccg gttcgaggag acgaccgcgt cggtcggtcc cgatctcgcg cggccggtcg
     4801 tcgcccgcgg cgcggcgtac gccgattacg accaggatgg cgatctcgac gtcgcctact
     4861 cggccaacgg tggcccagcg gtgctgctgc gcaacgacgg gggaggtgcg ggagggtgga
     4921 tgcggctgcg gctggtgggc tccccctcga accgtgacgc catcggcgcc cgcgtgcgcc
     4981 tcacctccgg gggccgcacc cagacccgca cggttcgggc ggcggcgtcg tacgcgtcac
     5041 agagcccgat ggtgctgacc ttcggcctcg gcggatccgg gaagggcgac gcggcgaccg
     5101 cagaggtccg ctggccggac gggtcgaagc aggccttcac cggactggcc cccggaaaaa
     5161 ccatggtcct gaagcaaggc agcccgaccg cgacggactg acgtccctcc ctggtctgcg
     5221 ggaggcgtga ccgcggacca atcaccgcgc cggccgcctc ttccccccag tcagacattg
     5281 acctgacgga tgggccggca tataccgggc gcactgccgg ttgcgggctc gatcatcgag
     5341 gtgaaccatg agacagcatc acgcccgaga tgcccgcctc ctgtccggat tggtccttgg
     5401 cgcagcgttc gcggcggcgt ccatggcgcg cgccggaacc gggctcttcg agcatcccgt
     5461 cttcccgacc ggcgaaatgc cgcgtcgagt tgcgatcggc gacttcaatg gcgacggggt
     5521 gccggacgtg gtgacggtca acgccgcgag cgacgacgtc tccgtcctgc tcggcgccgg
     5581 ggcggacggc ctcatgccgg agcggcgcta tcccgccggc gatctgccgt acggcctcac
     5641 gctgggggag ttcgacggcg acggcctcct cgacctggcc gtgacgaact gcgactcgcg
     5701 ggacgtcttc gtctacatcg ggcggggaga cgggcgcttt gcggtcaccg accggctgcc
     5761 ggcgggcggg tgcccgacgt ccatcctggc gagcgatctc gatgccgacg ggctccagga
     5821 tcttgctctg gtgaacgact tctcgaacga actgtggatc tatccgggcc tcggtggggc
     5881 gcgcttcggg cccgccgccg tgtctccgct cgatcgctgg cccatcctgg gtggggccgc
     5941 cgatttcgat cgggacggtc acatggacct gctcgtcagc gccgccgcgg agcgatccgt
     6001 cacggtcctg ttcggggatg gtgccttggc cttcccgcga cgggcggtgg tcccgatcgc
     6061 aggctgggtg ggggccctcg tcatccacga cttcgacggc gacggcagcc ccgacttcgc
     6121 cgcggctcac tttccggccg cgccgggtgg ggcggcgctc caggtgttcc ggggacaagg
     6181 cgacggcacc ttcacggccg tccaggacct cccgcccggg tatgagccac tcgtcctcgc
     6241 ggcggccgac ctcaccggcg acggcgccgc cgacctggtg gccctcaacg ggtaccccgc
     6301 cgtcctcgtg gtgttcgtcg gccatggcga cgcgacgttc cagtccgggg tggtcttcgg
     6361 caccggccca gaccccggag gtctcggcct tgcggatatc gatcgcgacg gccgtacgga
     6421 cgccctggcg accagcacga gcggccatgc cgtctggatg caccgcggtc tcggggacgg
     6481 tgggctcgaa acgacccggc tgttcgccgc gggcgatggg ccggaccggg tcgtgagcgc
     6541 cgatttcgat caggatggcg cggcggatct cgcggtagcc agcgcttacg gtgggatcga
     6601 cggcaagggc cacatcacga cgcttcaggg agacggcgtc ggggggttca cgccttcgca
     6661 cgatctggcg atcgccggga atgccgacga cctcgcaacc ggcgacctga acggcgacgg
     6721 cttcccggat ctcgcagccg cggcggtggg agaccggtgg ggcagccctc ccgtgcctag
     6781 tggcgcatcc atcctgctcg gagacggggc aggcgggttc actcccgaaa tcctcatcga
     6841 ggatccggat gacgagccga ggggcatcgc catcggcctg gtgaaccatg acgagacgcc
     6901 ggacatcgtc ctgagcggct cgctggtctc gacctggccc ggccgggggg acgggacgtt
     6961 cgccgcccct gtgcgctctg aggtggggac ctggccttcg cgcgcggttc tcgccgatct
     7021 caatggggac ggaacgaccg atcttgcggt cgccaatggg aacccggcgg tcatctgccc
     7081 gctgtggccc tatccctgcc cgccgccgaa gccgggtgac atttccgtcc tcctcggcct
     7141 gccaggagga ggcttcgccc cggaggtccg ctacgccgcc gggatgttcc cttcgtcgat
     7201 cgcggcgggg gacctcgatg atgacggggc gatggatctc gttgcgaatg gcgacgggga
     7261 cctgtccatc ttcatggggc agggggacgg gacgctggcc gcgcggcgga gggtcgcagc
     7321 cggcagtacc ccgggggcga ttctcatcaa cgatttcgac ggggacggcg tgctcgacgt
     7381 cgtgaccgct gacgccggcg gcaacacggt ctccgttctg ccgggccgcg gcgacgggac
     7441 ctttctcgcg agcacccagt accaggccgg cgtgcttcca ttcgattgtg cgagcggcga
     7501 tttcaacggc gacggtcgtc tcgacctcgc caccacgagc tactggccca atggagccgt
     7561 tgtcctcctg aaccagggtc cacccgacgc cgatggcgac ggcgtgatcg atcgcgagga
     7621 tccctgcacc gacctcgacg gcgacggcgc cggcgatccg gtcctcccgg cgaacacctg
     7681 tgccgcggac aactgtccgt cgctgcccaa ccccgcgcag gaggacgccg accacgacgg
     7741 cgtgggcgac gcgtgcgaca cctgcccgcg cgcgtccgac atggaacagc aagacaccga
     7801 ccgggacggc gtcggcgacg tctgcgacga ctgccccgac acgcccgacc cgctgcagtc
     7861 ggcggcgagc gacgcggact ccatcggtga tgcctgcgac aactgcccca cggtcgtgaa
     7921 ccccatgcag gaggacgcgg acggcgacgg cacgggcgat gcctgcgacc tctgctccga
     7981 tgccgacggc gacggctggg gcgacgcccg cttccctgca tccacctgcg ccgccgacaa
     8041 ctgcccggcg acctccaacc ccggtcagga ggacggcgac accgacggtc ttggagacgg
     8101 ctgcgacaac tgcccggctg cgtccaatgc cggccaggcg gatcgcgatg gcgacggcgc
     8161 gggggatgcc tgccagccgg cgctcgacct gcgcggtgtt cgaccgcacg ggcggacgtc
     8221 cctcgtcgcg ctcctctcgg ccagcgaccc gcagggggag ccgctgccgg gaacgctccg
     8281 gatcgagcgc ctgccgttca ccctggtcct gtcggatgcc ggacccatac cctcgtgcaa
     8341 cgaaggcttc ctgcccgagg atcaagccgg cgagggggtc ggcttcctgt acggcgacag
     8401 gagcgggccg gtcctgtacg acctcgactc gaacctcgga tgcgacgacg gcctcccgga
     8461 cttcggcatc gccgtcggcc catgcgatgc tcctctgacg atgttcttcg agctgctgga
     8521 gctgaacgcc tggagcaaca cgatccccga aaccctctgc gtccgcgccg tgggcgatcc
     8581 cggcggcgga ctcgaactga ccgtcgaggg gctcgaaccg gacagactcg tgacgacggc
     8641 ccgtgacacc actccggcgg tcagcatcga gttccagaac ggacctccgg acagcctgga
     8701 catcctgagc ctgagcgccg gcgccgacta ccgtctcgcg gtcacgatca cagacgggaa
     8761 cacgccgccc gtgaccgcac aggctgactt ccggcatcgg cgcgagaccg tgatgctcat
     8821 cggcctccca ccggcgatcg gcatgacggc gcgtccggtg gtccctgcgc gtcccacacg
     8881 tcccacacct gcgtcatgag gcgcccgcgg tcgccgctta cgaaattgca actccgcacg
     8941 cgccggattt tcgtgacgat tcgaggacgg cgttctcctt gccgcgactc ctgtggcaga
     9001 atctcctagc ctctgggttg gggggcgggt ctgagggagc cgctctctga agcgtgacga
     9061 gtcgctgaag ttcgtcgccg ggaacgtcgc gctcctcttt ttttattacg ccgccgggcg
     9121 gctggggctg gcgctcgcct acctccaccc gagcgcgtcg cccgtctggc ccccctccgg
     9181 gatcgccctc gcagccctgc ttgtcctcgg gtaccagtcc tggccgacga tttttctcgg
     9241 gtccttcctg gtcaacgtgg ccacggcggg gaaggtcgcc atgtcgctcg gcatcgccac
     9301 cggcaacacg ctcgaggccc tcttcgccgc gtacctcgtc aaccactacg cgtccggggt
     9361 caaggcgttc tacaggccgg aggacttcct gcgcttcggt ggactggcgg cgctcctgag
     9421 caccgccgtc agcgccaccc tcgggttcgc gagcctcgcc ctcgggggcg ctgtgcgctg
     9481 ggatcaggtc gcgtccgtct ggaccacctg gtggctcggc gacgcggtcg gcatcctgct
     9541 ggtgacgcca ttcctggtcc tctgggcggc gcggccgcag acaccgtggc gcggccgtga
     9601 gctggaagtg atcctcttga tgctgtgcac cgcgtcggtc ggctgggtgg tcttctccga
     9661 cgcgctgccg ctccggtcag ggagcctccc cctcggatat ctctgcatcc tgccgctcct
     9721 gtgggcggcg ctgcgcttcg ggcagcgcac ggtcgcgacg ggagtgctgg tgctgtcggc
     9781 ggtggcgctg tgggagacgg tggccgccct cggcggcgtt ggcggcgcgg cgcgggcggc
     9841 ggtcaacgag cggctgctcc tgctgcagct gttcctcggg tcggtgtcgg cgatggcggt
     9901 cggcgtcggt gcgtgggtgt cgcaggggag gagggcggag gaagagctct tgcgcagccg
     9961 ggagtggctc gagcggaagg tgatggagcg cacgctcgag atctccagcg ccagcgccgg
    10021 cctgcagcag agcgaggcgc gattccgcga gctgctcgag tcggccccgg acgccatggt
    10081 gctggtcgat cggagcggga ccatcgtcct ggtcaacgcc cagaccgaga agatgttcgg
    10141 ccggtcgcgc
//
LOCUS       DASRIQ010000302         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_651989, whole genome shotgun sequence.
ACCESSION   DASRIQ010000302 DASRIQ010000000
VERSION     DASRIQ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651989"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3186
                     /locus_tag="VFD06_07315"
     CDS             <1..>3186
                     /locus_tag="VFD06_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009413567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN03383.1"
                     /translation="GGPETDGSAVTLMTLDTSRVYWVERRPDTTSVIRRATLSDLEVM
                     SMADISNPGHLLHDDWYLFWDDDDSIEKIEKDTTIQRPDLFWQEDGLIDVIQALTTNR
                     TNSIELVADKRTIVRVYPGVDEGEVRLVYAELSGTRDGVDLPGSPLRPSQLYRTVDAG
                     PIDRTDLSKAIDFLLPTSWTSGQVHLEATLNRGDAIPESGNTLNNTATFDVTFVRKQP
                     LCLKMRKVSTDDPGEIDSNAVRFITYRALAMLPTPDILEDFIGGILRESTFWGATPEF
                     EMSSDAGSILDDLESEELDDTDPAFCRDADARSLRVGMITPDTTWNFSGMAHVPSNAS
                     IVMMSNSIGSGENPVFDFDDPAGTRTLVHEHTHCGGYRHVNCSGLEADGGEIDPAYPF
                     GCDATGANCCSISNDIGGLSDMGYDSRSQSIIEPTDASSYMTYKNFDSNDGTLNKRQW
                     ADSYHWSGLFDAYATLPDAAAVAAASMAAPMAADAIPPPGPLILLVQGTIDPVTGDVT
                     IRRLRRLDRAALPVEKVNGRWNDQEDNVNARGVRLVLEARHADDTLEFSLPFAPRGLG
                     SQTPITPEPFSVILPVSPLVTAVKILDPVDDSLKAFRTRSANTPVVTAVTSPLPGQVV
                     GNDLTVTWTAADADGEPLSYSVQYSHDSGATWQTLSTDLVETTVTYENEVHIPGSLNP
                     TQPGSSLVRVTASDGFNTGTHLSYPFQVLNRAPEAQIVLPLDDQRFAADEQIVLRARS
                     SDAETGSLDNGGVYDWDIVGFGAFTGPLVVLESGLPPGEWTINLTGRDPLGLTDSASI
                     TIFVDEFHTETPSDRDGDGVTDTADNCPGVPNPSQADSDGDGVGDVCDNCPFLVNRYQ
                     SDMDEDGMGDQCDPCPAGKVAEVSVDGVAEPGYGPPLAVQNTRTSAGDNSDPSPVTAN
                     GSELDTLHAVMDCDTLHILYTGNLSTASFGSGDTLDIFIDSTPGGENRLLGTHPHPAI
                     ARMGSTDSVNGLTFDAAFAADYFMRVRLVAQPGGGWRVAADYATLSGGGGGTAFDLGT
                     AAPDSDGHLTGVQPGAPTVLCTLNNSNTSGVS"
BASE COUNT          581 a         1189 c          946 g          470 t
ORIGIN      
        1 cggcggcccc gagacggacg gctcggccgt caccctgatg accttggaca cgtcgcgcgt
       61 ctactgggtc gagcggcgcc ccgacacgac gtcggtcatt cgccgggcca ccctgtcgga
      121 cctcgaggtc atgtccatgg ccgacatctc caaccccggc cacctcctcc acgacgactg
      181 gtatctcttc tgggacgacg acgacagcat cgagaagatc gaaaaggaca ccacgatcca
      241 acgccccgac ctgttctggc aggaggacgg cctcatcgac gtgatccagg cgctgacgac
      301 caaccgcacc aactccatcg agctcgtcgc ggacaagcgc acaatcgtcc gggtctatcc
      361 cggggtggac gagggagagg tgcgtctcgt ctacgcggag ctgagcggca cgcgagacgg
      421 cgtggatctg cccggctcgc cgctgcgccc atcccagctc taccggaccg tcgacgccgg
      481 ccccatcgat cggaccgacc tgagcaaggc gattgacttc ctcctgccga cgagttggac
      541 cagcgggcag gttcacctgg aggccaccct caaccggggc gatgccatcc cagaaagcgg
      601 aaacacactc aacaacaccg ccaccttcga cgtgacgttt gtccggaagc agccgctgtg
      661 cctgaagatg cgcaaggtgt ctacggacga cccgggtgag atcgactcca acgccgtcag
      721 attcatcacc tatcgcgccc tggcgatgct gccgacaccg gacatcctcg aggatttcat
      781 tggcggcatc ctcagggaaa gcacattctg gggcgccacc ccggagttcg agatgagcag
      841 cgacgcaggc tctatcctcg acgacctcga gtcggaggag cttgacgaca cagacccagc
      901 cttctgccgc gatgccgacg cccgttcact ccgcgtcggg atgatcaccc ccgacacgac
      961 atggaacttc agtggcatgg cacacgttcc cagcaacgcc agcatcgtca tgatgagcaa
     1021 ttcgatcggc agcggcgaaa accccgtctt cgacttcgac gatccggcgg gcacacggac
     1081 gctcgtccac gaacacaccc actgcggcgg ataccgccac gtcaactgct cgggactgga
     1141 ggccgacggc ggcgaaatcg acccggccta ccccttcggc tgcgacgcca ccggggcgaa
     1201 ctgctgctcc atctccaacg acataggtgg tctgagcgac atgggatacg acagccgctc
     1261 tcagtcgatc atcgagccga ccgatgcgtc gtcgtatatg acgtacaaga actttgacag
     1321 caacgacgga acgctgaaca aacgccagtg ggcagactcg taccactgga gcggcctctt
     1381 cgacgcttac gccacgctgc cggacgcggc cgcagtggcc gccgccagca tggccgcgcc
     1441 catggccgcg gatgccatcc ccccgcccgg ccccctgatc ctcctcgtgc aggggaccat
     1501 cgatccggtc acgggtgacg tcacgatcag gcgcctccgc cgcctcgatc gcgccgccct
     1561 gcccgtcgag aaagtcaacg gccgatggaa cgaccaggag gacaacgtca acgccagagg
     1621 cgtccggctg gtcctcgaag cccgccatgc ggacgacacc ctggagttct ccctcccgtt
     1681 tgccccgagg ggcctgggca gccagacgcc gatcacgccc gagccgttct cggtgatcct
     1741 ccccgtctcg ccgctggtga ccgcggtgaa gatcctcgat cccgtggacg attccctgaa
     1801 ggcgtttcgc acccggtccg cgaacactcc cgtggtgacc gccgtcacat cgccgctccc
     1861 cggccaggtc gtcggaaacg acctgaccgt cacatggacc gccgcggacg ccgacggcga
     1921 gccgctctcc tattccgtcc agtactcgca tgacagcggc gcgacgtggc agacgctctc
     1981 gaccgacctc gtcgagacca ccgtgaccta cgagaacgag gtgcacattc ccggcagtct
     2041 caacccgaca cagccgggga gctcgctcgt ccgggtcacc gcgagcgacg gcttcaacac
     2101 cggcacgcac ctctcctacc cgttccaggt gctgaaccgg gcgccggagg cccagatcgt
     2161 cctgcccctg gacgaccagc ggttcgccgc cgacgagcag atcgtcctgc gcgcccgctc
     2221 ctccgacgcc gagaccggct cactcgacaa cggcggcgtg tacgactggg acatcgtcgg
     2281 cttcggcgcc ttcaccggcc ccctggtcgt gctggagagc ggcctcccgc cgggagagtg
     2341 gaccatcaac ctcaccggcc gcgacccgct gggcctcacg gactccgcgt ccatcacgat
     2401 cttcgtcgat gagttccaca cagagacgcc gagcgaccgt gacggcgacg gcgtgaccga
     2461 caccgcggac aactgcccgg gggtccctaa cccctcccag gccgactcgg acggcgacgg
     2521 agttggagat gtctgcgaca actgcccgtt cctcgtcaat cgctaccaga gcgacatgga
     2581 tgaggacggg atgggggatc agtgcgatcc gtgcccggcc ggcaaggtcg ccgaggtctc
     2641 cgtggacggc gtcgccgagc ccggttacgg cccgcccctg gcggtgcaga acacccggac
     2701 ctccgccgga gacaactccg acccctcgcc cgtcaccgcg aacggatccg agctcgacac
     2761 cctgcacgcc gtgatggact gcgacaccct gcacatcctc tacacgggca acctctccac
     2821 ggccagcttc gggagcggcg atacactcga catcttcatc gactccactc ccggaggtga
     2881 gaaccgcctc ctcgggacgc acccacaccc ggcgatcgcg cgcatgggct ccaccgactc
     2941 ggtcaacggg ctcaccttcg acgccgcctt cgccgcggac tacttcatgc gcgtccgcct
     3001 cgtcgcccag ccgggcggtg gatggcgggt cgccgcggac tacgccacgt tgtccggtgg
     3061 cggcggaggc acggccttcg acctcggcac cgccgcgccc gactccgacg gtcacctcac
     3121 cggcgtgcag ccgggagcgc ccactgttct ctgcacgctc aacaactcca acaccagcgg
     3181 cgtgtc
//
LOCUS       DASRIQ010000303         5971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1685706, whole genome shotgun sequence.
ACCESSION   DASRIQ010000303 DASRIQ010000000
VERSION     DASRIQ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685706"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFD06_07320"
     CDS             complement(<1..592)
                     /locus_tag="VFD06_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZN03384.1"
                     /translation="MDSAANGRARARESASRDDDSTEAEITKVSAPPAPKPPAAAPAR
                     TAETLSITDNRTGKSYEIPIQDGTIRAMDLRQIKIKPEDFGIMSYDPAFTNTAACRSA
                     ITEIDGDKGILNYRGYPIEQLAEESSYLETAYLILHGELPARQQYEEWVHNITHHTIL
                     HENIKKFIDGFRYDAHPMGMLISTVAAMSTFYKDAKD"
     gene            complement(722..1501)
                     /locus_tag="VFD06_07325"
     CDS             complement(722..1501)
                     /locus_tag="VFD06_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZN03385.1"
                     /translation="MRAADPPVVRPGDYARRLKPGERFEVAWDDHRRYHPRPAERPGR
                     GYNVLHRDDALIVIDKGPEVLSVPTALRSEDSLLERLLEAEKERGIRNAQIFPVHRLD
                     RDTSGLLLFARTGPALGGLKEQFADRSLERRYVAVVEGSLEKASGRMESRVVEDRKSL
                     KVRSTGAPGRGGRRSPNTRSPSASRARPWSTCACAPAGRTRFASTSPRQATRSWVTGG
                     MAGPAVTSPGPPSTRCVWPSSIRSRTSASPSRAPSRPTSGT"
     gene            complement(1623..1961)
                     /locus_tag="VFD06_07330"
     CDS             complement(1623..1961)
                     /locus_tag="VFD06_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN03386.1"
                     /translation="MKISSRDVGKVKVLDLKGRITLGAGDLELRKAVQDELDKGNNNI
                     LLAMQGVDYVDSAGLGELVAVKKRAGAKGGTVKLVMPNESVYKVMSIVSLHLVFEIFD
                     SEAKAIASFA"
     gene            2117..4054
                     /locus_tag="VFD06_07335"
     CDS             2117..4054
                     /locus_tag="VFD06_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03387.1"
                     /translation="MNVGLHVLMLLVGTAASTDVAGDPLPFRVPAAVFVVTSTADGGP
                     GSLRQAILDANASPGADAVHFDIAGGGVKTIAPLTPLPIVSDPLTIDGTTQPSFAGTP
                     LIELSGAMAPSAVGLEIAAGSSVVRGLAVNRFAYGIALTTNGGNLVEGNFIGTDPSGT
                     IDRGNVLFGIRVTGGSGNIIGGTSVSSRNVISGNDNHGVGLVSGGNVVLGNYIGTDRT
                     GMSALPNRIGLLTENSANVSNTIGGTSAGAGNLISGNSGAGVSLGGTVLGHLVQGNWI
                     GLDASGTGDLGNGGDGIALQFGSCCNSIGGTAAGAGNVISGNNLNGIRSDYHPQAIRG
                     NRIGTTADGTAPLGNDGHGVYVNTMSNNVVGGIAPGAGNTIAWNGGDGVALGNAGFGC
                     AVLGNSIRANDGLGINLGPNGVTPNDPGDGDAGPNGLQNFPVLMAVRDLGAETLVEGV
                     LESRPGTAFVIEVFAAGGCDPSGHGEGERPIGSSTLTTGADGKVPFSLSFPTAVPAGS
                     EVTATATDPANSTSEFSACRQAVCAASIPQGSPVLQVEADHLAWSALAGAGAYDLVRG
                     DLATLRATGGDFTASTLDCLAGGLGSAAYPFSANPSPGEGFWFLVRGINCAGDGSYDS
                     GAASQIGTRDAEIAGSAGACP"
     gene            complement(4162..4824)
                     /locus_tag="VFD06_07340"
     CDS             complement(4162..4824)
                     /locus_tag="VFD06_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN03388.1"
                     /translation="MDDDLELRRLIAFTLRESGYLIVEAGDGEAALKVFEEEKPDLVL
                     LDVNMPRANGFDVLKRLRESGARTPVMMLTVRAAEEDQVKGLDLGADDYLAKPFSPRT
                     LLARVRALLRRSADGRESKLEAGDLVLDPENRGVSVRGGDPVRLTALEFRLLQLLVAN
                     AGHTLPTERLTVHVWGRRGTGDRQMLKQLVHRLRRKIEKDPAEPRYLKTVSGVGYTLE
                     VG"
     gene            complement(4836..>5971)
                     /locus_tag="VFD06_07345"
     CDS             complement(4836..>5971)
                     /locus_tag="VFD06_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN03389.1"
                     /translation="LTAESRRRRKDAEAILGGISEGVYAVDRERRFRFLNRQTAELLG
                     IDPKEALGRFCGDVLNPQGPEGIRPCEDNCPILDARFRGGARATERLLLKNGSIRTVV
                     INSAPPGLPEEEGEGGVGPGTPGPGRQFQLIRDETEIEATRRLRDAVLANITHEFRTP
                     LSAQLASLELLRERMPENATPETLELLSSLERGALRLTQLIDNLLESVRIDAGNQDIR
                     RQPLALDEVIEEAAQATAPLLELRGQRLEVDLPYPLPPIQGDAPRLVQVFVNLIANAN
                     KFAPTGTLIKVGGQVDRTSVTLWVEDQGPGFPAEDGVLLFEPFTRSPGEEPEQTGVGL
                     GLYIVKSIVERHGGHVEARPAKGRGARLTVTLPFDGQARAAQA"
BASE COUNT          846 a         2032 c         2122 g          971 t
ORIGIN      
        1 tgtccttggc gtccttgtag aacgtggaca tcgcggcgac cgtcgagatc agcatcccca
       61 tcgggtgcgc gtcgtagcgg aagccgtcga tgaacttctt gatgttctcg tggaggatgg
      121 tgtggtgcgt gatgttgtgc acccactcct cgtactgctg ccgcgccggc agctccccgt
      181 gcaggatgag gtaggcggtc tcgaggtagg agctctcctc cgccagctgc tcgatcgggt
      241 agccgcggta gttgaggatc cccttgtccc cgtcgatctc ggtgatggcg ctgcggcagg
      301 cggccgtgtt ggtgaaggcc gggtcgtacg acatgatccc gaagtcctcc ggcttgatct
      361 tgatctgccg caggtccatg gcccggatgg tgccgtcctg aatcgggatc tcgtacgact
      421 tgccggtgcg gttgtcggtg atcgagaggg tctcggcggt gcgggcgggg gccgcggccg
      481 gcggcttcgg ggccggaggg gccgaaacct tggtgatttc cgcctcggtc gagtcgtcgt
      541 cccgggacgc cgactcgcgc gcgcgcgccc tgccgttcgc tgcgctgtcc atgtccaccc
      601 tcttccccag cctttactgt gcggaaccac tgaaggataa cccaatggcc ggtcccgcgg
      661 gtatgcgatc tcggcaaact tgtggtgtcg gatcgcccga gtcaacctcg gaggcggcgg
      721 atcaggtccc ggatgtcggc cgggaggggg ctctcgaagg agacgcgctg gttcgtgacc
      781 ggatggatga aggccagacg cagcgcgtgg agggcggtcc gggcgatgtg acggctgggc
      841 cggccatacc gccggtcacc cacgaccggg tggcctgcct cggcgaagtg gacgcgaatc
      901 tggttcttcc ggccggtgcg caggcgcacg ttgaccacgg tcgcgcccgg gaggcgctcg
      961 gcgacctcgt attcggtgat cgcctcccgc cccttcccgg ggcgccggtg gaccggacct
     1021 tgaggctttt ccggtcctcg acgacccggc tctccatccg gccggaggcc ttttcaaggg
     1081 atccctcgac gaccgccacg tagcgccgct ccagcgaccg gtcggcgaac tgctccttca
     1141 ggccgccgag ggcggggccg gtccgggcga acaggagcag ccccgaggtg tcccggtcga
     1201 ggcggtggac cgggaagatc tgggcgttcc ggattccccg ctccttctcg gcctcgagca
     1261 gccgctcgag gaggctgtcc tcggaccgca gcgcggtcgg gaccgacagg acctccggcc
     1321 ccttgtcgat gacgatcagg gcgtcgtccc ggtgcaggac gttgtagccg cggccggggc
     1381 gctcggcggg ccgggggtgg tagcgtcggt gatcatccca ggcgacctcg aagcgctccc
     1441 ccggcttgag gcggcgggcg taatcccccg ggcggacaac gggcgggtcg gcggcgcgca
     1501 cggcaccggc gtcgatgagt ccgcgggcct gcttgtggct gaggccgcag cggtcgtgga
     1561 ggagcgcctg gagcgtccgg ccgacgtcgg ccggcccggc ttcgacggtg accttcttca
     1621 gttcaggcga aggaggcgat cgccttcgcc tcggaatcga agatctcgaa caccaggtgc
     1681 agggagacga tcgacatgac cttgtagacc gactcgttcg gcatgacgag cttcacggtc
     1741 ccgcccttgg cgcccgcgcg cttcttcacc gcgaccagct ccccgagccc ggccgaatcg
     1801 acgtagtcca ccccctgcat ggccaggagg atgttgttgt tccccttgtc cagctcgtcc
     1861 tggaccgcct tgcgcagctc caggtccccc gccccgagcg tgatccgccc cttcaggtcg
     1921 agcaccttca cctttccaac atccctgctg ctgatcttca tttttctcct ggtccccggc
     1981 gtcgcccatg cccggaagcc cggatgatgg cacaggggga ggggggatgc caagcgggat
     2041 tggctccggg cgcttgactg cggcgcgctg ccgtgctgca ctagcactct cggagcgcca
     2101 ggcgggtgcg atggtcatga acgtcggctt gcatgtgctg atgttgctcg tcggcacggc
     2161 tgcgtcgact gacgtcgctg gcgatcctct cccgttccgg gtcccggctg ccgtgttcgt
     2221 ggtcaccagc acggcggacg gcgggccggg ctcgctgcgg caggcgattc tcgacgccaa
     2281 cgccagcccg ggagcggacg ccgttcactt cgacatcgcc ggaggcgggg tcaagacgat
     2341 cgccccgctg actcccctgc cgatcgtcag cgacccgctg accatcgacg gaaccacgca
     2401 gccctcgttc gcggggactc cgctgatcga gctgagcggc gccatggcgc cgtccgccgt
     2461 cgggctcgag atcgcggcgg ggagcagcgt cgtccgggga ttggcggtca accgtttcgc
     2521 gtacgggatc gcgctgacca cgaacggcgg caacctcgtc gaggggaact tcatcggcac
     2581 ggacccgtcc gggacgatcg accggggcaa cgtcctgttc ggcatccggg tcaccggcgg
     2641 ctcgggcaac atcatcggcg ggacgagcgt ctcctcccgc aacgtcattt cgggcaacga
     2701 caatcacggc gtcggcctcg tctccggcgg caacgtcgtc ctcgggaact acatcggtac
     2761 cgaccgcacg gggatgagcg ccctcccgaa ccggatcggt ctcctgaccg agaacagcgc
     2821 gaacgtcagc aacaccatcg gcgggacctc ggccggcgcg ggcaatctga tctccgggaa
     2881 cagcggcgcc ggagtgtcgc tgggcgggac ggtgctgggt catctggtgc aggggaactg
     2941 gatcggtctc gatgcctcgg gcacggggga tctcggcaac gggggcgacg ggatcgcgct
     3001 ccagttcggt tcctgctgca actccatcgg cggaacggcg gccggggccg ggaacgtcat
     3061 ctccgggaac aacctcaacg gcatccgctc cgactaccat ccgcaggcga tccgcggaaa
     3121 ccgcatcggg acgaccgccg acggaacggc gccgctcggc aacgacggcc acggcgtcta
     3181 cgtcaatacg atgagcaaca acgtggtcgg cggcatcgca ccaggggcgg ggaacaccat
     3241 cgcgtggaac ggcggcgacg gagtcgctct tggcaacgcc gggttcgggt gtgccgtgct
     3301 cggcaactcc atccgtgcga acgacgggct cggcatcaac ctcggcccga acggcgtcac
     3361 gccgaacgac cccggggacg gcgatgcggg ccccaacggc ctgcagaact tcccggtgct
     3421 catggcggtg cgcgacctcg gcgccgagac cctggtcgag ggcgtcctgg aaagccggcc
     3481 cggcacggcc ttcgtcatcg aagtgttcgc ggcgggcggt tgcgacccct ccgggcacgg
     3541 cgaaggagag aggccgatcg gttcctcgac cctcacgacg ggagccgacg gcaaggtgcc
     3601 gttctccctc tcctttccga ccgccgtccc ggccggatcg gaagtgacgg cgacggcgac
     3661 cgatccggcg aacagcacgt ctgagttttc cgcctgccgg caggcggtct gcgccgcatc
     3721 gatcccgcag gggtcccccg tgctgcaagt cgaggccgac cacctcgcgt ggagcgcgct
     3781 ggccggtgcc ggggcgtacg acctggtgcg cggcgatctg gccaccctgc gcgcgaccgg
     3841 cggcgacttc accgcttcga cgctcgactg cctcgcgggc ggtctcggat cggccgctta
     3901 ccccttcagc gcgaatccat cccccggcga gggattctgg ttcctggtgc gcggaatcaa
     3961 ctgcgccggc gacggttcct acgactcggg agcggcgtcg cagatcggca cgcgcgatgc
     4021 cgagatcgcc ggctccgcgg gcgcctgtcc ctgagcagga acggaatccg accgcccgcg
     4081 cgacagggcc aagatcaacc gggccggccg ggatgatggc actggcggcg ggatgcccag
     4141 cgacgggccc ctggacgtga cctaaccaac ttcaagcgta tagccaactc cgctcacggt
     4201 cttaaggtat cgcggctccg ccgggtcttt ctcgatcttc ctcctcagcc ggtgcacgag
     4261 ctgcttcagc atctggcggt cgccggtgcc gcggcgaccc cagacgtgga cggtgaggcg
     4321 ctcggtgggg agggtgtggc cagcgttcgc gaccagcaac tgaagcaggc gaaactcaag
     4381 ggcggtgagg cggaccgggt cgccgccgcg gacggagacg ccgcgattct cggggtcgag
     4441 gacgaggtca ccggcctcaa gcttcgattc acgcccgtcg gcgctgcgac gcagcagggc
     4501 gcgcacgcga gccagcaggg tgcgtgggct gaacggcttg gcgaggtagt cgtcggcgcc
     4561 gaggtcgagg cctttgacct ggtcctcctc ggcggcgcgc accgtgagca tcatgaccgg
     4621 ggtgcgggcg ccgctctcgc gcaggcgctt caggacgtcg aagccgttgg cgcgcggcat
     4681 gttcacgtcg aggaggacga ggtcgggctt ctcctcctcg aaaactttca aagcggcctc
     4741 gccgtcgccg gcttcgacga tgaggtagcc ggactcgcgc agggtgaagg cgatgaggcg
     4801 gcgcagctcg aggtcgtcgt ccaccacctc gattttcatg cctgcgccgc gcgtgcctgg
     4861 ccgtcgaacg gcagggtcac cgtcagccgc gcgccccggc ccttggcggg gcgggcctcg
     4921 acgtggccgc cgtggcgctc gacgatcgac ttgacgatgt agaggccgag cccgacgccg
     4981 gtctgctctg gctcctcccc gggcgagcgg gtgaacggct cgaacagcag cacgccgtcc
     5041 tccgccggga agccgggccc ctggtcttcc acccagagcg tgacgctcgt gcggtccacc
     5101 tggccgccga ctttgatgag agtgccggtc ggcgcgaact tgttggcgtt ggcgatcagg
     5161 ttgacgaaca cctgcacgag gcgcggcgcg tctccctgga tcggcggcag cgggtacggc
     5221 agatcgacct ccagccgctg gccgcgcagc tcgagcagcg gcgccgtggc ctgcgccgcc
     5281 tcctcgatca cttcgtcgag ggcgagcggc tggcggcgaa tgtcctgatt gccggcgtcg
     5341 atccggacgc tctccagcag gttgtcgatg agctgcgtca accgcagcgc gccgcgctcg
     5401 agcgacgaca gcagctcgag ggtctccggc gtggcgttct ccggcatgcg ctcgcgcagc
     5461 agctccagcg acgccagctg cgcgctgagc ggagtgcgga actcgtgggt gatgttggcg
     5521 aggaccgcat cgcgcagacg gcgggtcgcc tcgatctcgg tctcgtcccg aatcaactgg
     5581 aactgccggc ctgggccggg cgtccccggt cccacccccc cttccccttc ctcttcaggg
     5641 aggccgggcg gggcgctgtt gatgaccacg gtgcggatgg agccgttctt gaggagcagc
     5701 cgctccgtgg cgcgggcgcc gccgcggaag cgggcgtcga ggatggggca gttgtcctcg
     5761 cacgggcgga tcccttccgg cccctgcggg ttcaggacgt cgccacagaa gcggccgagc
     5821 gcctctttcg ggtcgatgcc gagcagctcc gccgtctggc ggttcaggaa ccggaagcgc
     5881 cgctcccgat ccacggcgta caccccttcg gagatgccgc cgaggatcgc ttcggcgtcc
     5941 ttgcgtcggc ggcgcgattc ggccgtcagc t
//
LOCUS       DASRIQ010000304         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2454471, whole genome shotgun sequence.
ACCESSION   DASRIQ010000304 DASRIQ010000000
VERSION     DASRIQ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454471"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFD06_07350"
     CDS             complement(<1..738)
                     /locus_tag="VFD06_07350"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HZN03390.1"
                     /translation="MAGPAIRALRLATVCVALAASASGGGVLALDRQKALTQFVLDSW
                     QMEDGLPQSAVHAIVQSRDGFLWAGTEEGLVRFDGTGFKVYDTSNTPGLRNDHIWALA
                     EDRDGTLWIGTYGGGLTHMRDGAFTALTTKEGLPSDSVRTLYADRSGATWVGTDGGGL
                     ARVEGGRVTAFTTREGLPHDRVNAIVEDGGGALWVGTDDGGLARLEEGRFHADPARHR
                     LHQEDVNALHVDRSGALWVGTDGGLARF"
     gene            933..2291
                     /locus_tag="VFD06_07355"
     CDS             933..2291
                     /locus_tag="VFD06_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN03391.1"
                     /translation="MPENPGAVEFRRELGLLDAAVVVAGAIVGVGIFANPSNVARIVG
                     EPILIVAAWIAGGALALLGGFAYAELASRLPVVGGQYVYLARAWHPVVGYLYGIALLF
                     IINGGSIAAVAILFASYLDASFLPLGPVGIRIAAAAVLVTLTAINVVGIRAGKRVNNA
                     LMAVKVFGILALVAVAFLGTGSSASRYDVSVLASPSASWMGLLFTALVPIMFAYGGWQ
                     NCGSVAGEIRDPVRNLPRANVLGVIVVIVLYVGLNLAYLRVLTPVQMAASPALASDAA
                     RTVAGEAGARFVGALILVSALGFLAVIILTAPRLYYAMARDGLFLSRAGTLHPRWHTP
                     VFMLWFQAAVSIALLATNTYDQLLSYVVFADWLFFGLTVGAIFVLRRRQAGVPGLVAM
                     PGHPFTTLLFVAAAAGIVVSCFLAYPMQSLKGSAILLLAAASFPLIAHRPSRAPRREA
                     GE"
     gene            2293..>2837
                     /locus_tag="VFD06_07360"
     CDS             2293..>2837
                     /locus_tag="VFD06_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN03392.1"
                     /translation="MKFPEIAYMSWAKAMPPARSNLARSGLDPCPPGLLKLTARDLAT
                     HHPDGYGWPPLLQALGRRYRVSPRRVLTVSGGTSLANWVACAAALDGAGADAEVIVER
                     PTYEPLLRIPESMGGTIRRLDRWHEEDWRIDPVRFASLVTPRTRLAIVTNLHNPSGAR
                     IDPGTLREIARSLERVGGYLLV"
BASE COUNT          347 a         1025 c          977 g          488 t
ORIGIN      
        1 gaatcgcgcg agcccgccgt cggtgccgac ccacagcgcc ccgctccgat cgacgtgcag
       61 cgcgttgacg tcctcctgat gcagccggtg acgggcggga tcggcatgga accgcccctc
      121 ctcgagccgg gcgagcccgc cgtcgtcggt gccgacccag agcgcgccgc cgccgtcctc
      181 gacgatcgcg ttgacgcgat cgtgcggcag accctcgcgc gtcgtgaacg ccgtgacgcg
      241 ccccccctcg acccgggcca gcccgcctcc gtcggtgccg acccacgtcg ccccggagcg
      301 gtcggcgtag agcgtccgca cgctgtcgct cgggaggccc tccttcgtgg tgagcgcggt
      361 gaaggctccg tcccgcatgt gggtgaggcc acccccgtag gtcccgatcc agagggttcc
      421 gtcgcgatcc tcggcgagcg cccagatgtg atcgttccgg agacccggcg tgttcgaggt
      481 gtcgtacacc ttgaaaccgg tgccatcgaa gcggaccagc ccctcctccg tcccggccca
      541 gagaaaaccg tcccgggact ggacgatggc gtgcacggcg ctctgcggga ggccgtcctc
      601 catctgccag gagtcgagga cgaactgggt cagcgccttc tgccggtcga gggccagcac
      661 gcccccgccc gaggcggagg cggccagcgc gacgcagacc gtcgccaggc gaagtgcgcg
      721 gatcgcggga cctgccatcg aaacccctgg cgccatccct tctgaagcga tcgtgtggat
      781 tcgggccgca tgctacctcc ggcggcgggt ggcgggtcaa gccgggagcg cgtcagcccg
      841 gtttgagatg ccgatcggtt gcatgagacg ggaacaggcc gaaatagaat ccaccccgtg
      901 agcctgatcg agcgtccgag cggcgcgcgc tgatgccgga gaaccccggc gcggtggagt
      961 tccggcgcga gctgggtctc ctggatgcgg cggtcgtggt ggccggggcg atcgtcggcg
     1021 tcgggatctt cgccaaccct tccaacgtcg cccggatcgt cggcgagccg atcctgatcg
     1081 tcgccgcgtg gatcgccggt ggcgcgctcg ctctcctcgg gggattcgcc tacgcggagc
     1141 tggcgtcgcg actgccggtc gtcggcgggc agtacgtcta cctcgcccgc gcctggcatc
     1201 cggtcgtcgg ttacctttat ggaatcgcgc tcctcttcat tattaatggg ggctcgatcg
     1261 ccgctgtcgc cattctgttc gcctcctatc tcgacgcttc gttcctgccg ctggggccgg
     1321 tgggaatccg catcgccgcc gcggccgtgc tggtgaccct cactgcgatc aacgtcgtcg
     1381 gcatccgtgc ggggaagcgc gtgaacaacg cgctcatggc ggtgaaggtc ttcgggatcc
     1441 tcgctctcgt ggcggtcgcc ttcctcggca ccggttcgtc ggcgagccgt tacgacgtgt
     1501 cggtcctcgc ttcgccgagc gcgtcatgga tggggctcct cttcacggcg ctcgtcccga
     1561 tcatgttcgc gtacgggggc tggcagaact gcggctcggt ggcgggggag atccgcgacc
     1621 cggtgcgcaa cctgccgcgg gcgaacgtcc tcggcgtgat cgtggtgatc gtgctgtacg
     1681 tcggcctcaa cctcgcttac ctgcgcgtgc tgacgccggt gcagatggcc gcgtcccccg
     1741 cgctcgcctc cgacgcggcc cggaccgtgg cgggagaggc gggcgcgcgc ttcgtcggcg
     1801 cgctgatcct cgtctcggcc ctcgggttcc tggccgtgat catcctgacg gcaccgcgtc
     1861 tctactacgc gatggcgcgt gacgggctct ttctgagccg cgccgggacg ctgcatccgc
     1921 gctggcacac gcccgtcttc atgctctggt tccaggcggc cgtctcgatc gccctcctcg
     1981 ccacgaacac ctacgaccag ctcctttcct acgtcgtctt cgccgactgg ctcttcttcg
     2041 ggctgaccgt gggggcgatc ttcgtcctgc gccgccggca ggccggcgtc ccggggctcg
     2101 tcgcgatgcc gggccatccc ttcaccacgc ttctcttcgt ggccgccgcc gcggggatcg
     2161 tcgtcagctg cttcctcgcc tatcccatgc agtcgctcaa gggatcggcg atccttctgc
     2221 tggcggcggc ctccttcccg ctgatcgctc ataggccctc gcgtgcgccc cgccgcgagg
     2281 caggagagtg acgtgaagtt ccccgagatc gcctacatga gctgggcgaa ggcgatgccg
     2341 ccggcgcgga gcaatctcgc gcgcagcggc ctcgatccgt gtccgcccgg cctgctgaag
     2401 ctcactgcgc gcgacctggc gacgcaccac ccggatgggt acggctggcc gccgctcctc
     2461 caggccctcg gccgccgcta ccgcgtgtcg ccccgccggg tcctgaccgt ctcggggggg
     2521 acgagcctcg ccaactgggt cgcgtgcgcg gcggccctgg acggggcggg cgcggacgcg
     2581 gaggtgatcg tcgagcgccc gacgtacgag ccgctcctgc gcatcccgga gtcgatgggc
     2641 ggcacgatcc ggaggctcga tcgctggcac gaggaggact ggcggatcga tccggtccgc
     2701 ttcgcctcgc tcgtcacgcc gcgcacgcgc ctggccatcg tgacgaatct tcacaacccc
     2761 tcgggggccc ggatcgatcc gggaacgctc cgggagatcg cgcggtctct cgagcgcgtg
     2821 ggtggatacc tgctggt
//
LOCUS       DASRIQ010000305         3980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_899818, whole genome shotgun sequence.
ACCESSION   DASRIQ010000305 DASRIQ010000000
VERSION     DASRIQ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899818"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFD06_07365"
     CDS             <1..1107
                     /locus_tag="VFD06_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HZN03393.1"
                     /translation="GVRGGAVHTFSIGFGEKDFDETPFARKVAAALGTVHREEILRPD
                     CLDLVQQAAGFLDEPFADNSILPTYLVSRVARESVKVALSGDGGDELFAGYDHYKAER
                     LLEGYARIPAPVRRGALTLLGRDAERRPTKRGGVRRRLRRLEEALAQPAFLAQARFMV
                     RSRDEMREGLLPDPGPDDPAAGWAEPFAAVVRDSPFPPGLAHQQFIDLKTFLAEDILF
                     KTDRASMAVSLEARVPYLDHELVEFAFRLAPSLKLRGLVGKWILRRAFRDRLPASILR
                     RRKSGFSVPIAAWLRHDLKTLARDLLAPGRVTRQGLFDARRVSAVLEEHLDGRADHAR
                     LLWALVMFQLWHERWSAAAPAAAVVPIAADEGVA"
     gene            1107..3314
                     /locus_tag="VFD06_07370"
     CDS             1107..3314
                     /locus_tag="VFD06_07370"
                     /inference="COORDINATES: protein motif:HMM:NF020704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN03394.1"
                     /translation="MQDDRLLNLSYWTELASRRRWLILGCTAGVFVSATAFSLMRPNL
                     YEATSTLLLSRQRVQAFDAQDLYTKQQAGTRPNEVVAAQIEILKSMPVLERAVHDLEE
                     RDLLHFDRPDPGDKPPLVSRVLIALHLRAPEPPPTPDERRRGYVRDLKDRMSTGTRGG
                     NAFLTVSVQSRNPEMAAETANAITNAYLRHTRELMRRSADDAVEWLSGKLREQRDKVL
                     ETEERLRSLSGPAPQAEDMDRLTVQEMSRIQAALLDVRLMMLRAETGGPVAGSGAAAP
                     PANNSVDDAVNGALRDKVQQELVETVSTLSRLRRTYGENHPDVIAAAEKEQQLRAELE
                     RLNTLAPRPRSNDPMGRITPSDAQALKAQERLLQQQLNITLQSSSAKGEAAMHYAILK
                     KEVEINRSLYNEMLTRLNEITIASGLDPGSAETFEPARIPRAPFSPQHTQTILLGFAA
                     GLLLGLAGAALRDHMDQSLRDPGQANDLLRAPVLGIIPDHRSLVGRPEPRRGTRMWVG
                     HGEESVSAEAYRVLRSHIEGTLTAEESRILILTSAVAGEGKSTTAANLAAAFAESGRR
                     VLLIDADFRRPTLRKVLGVELPEDHQLSLVLSGSKPPEKVILDSGIPDLDFIGHRANG
                     KIPDGSKTTESFKKLFAWGRDRYDRIIVDLPVLMIAPGVTELGRAGGTVLLVHRPGWV
                     PAPVLESVRQHLAMAKTRLVGAILNGTGARWASSQYLPVYYHAAYYSRKIEAP"
     gene            3335..>3980
                     /locus_tag="VFD06_07375"
     CDS             3335..>3980
                     /locus_tag="VFD06_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019980469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN03395.1"
                     /translation="MSSRRGRLWPVIARVAAWSGLGPAAGVLWRRRAAGTWTILGYHR
                     VGEPSDDGGPDAVRPERFRDHLRWLRARYDVMTVGEGLAALRRGGARRPMVSLTFDDG
                     YRDNVVHALPILLAEGCRATLYPTLEAIEEGRAPWPHRLALDLRRLTRRGDARPDSWP
                     EPLAALAGAAGLGPREVSERIDRIVSEAKRWPSEECSRITAEVERLAGPAASRPE"
BASE COUNT          569 a         1427 c         1446 g          538 t
ORIGIN      
        1 ggggtgcgcg gcggagcggt ccacaccttc tcgatcgggt tcggagagaa ggacttcgac
       61 gagacgccgt tcgcccgcaa ggtcgccgcg gcgctcggca ccgtccaccg cgaggagatc
      121 ctgcggcccg actgcctcga cctggtgcag caggcggccg gcttcctcga cgagccgttc
      181 gccgacaact cgatcctgcc gacgtatctc gtgtcgcgcg tggcgcgcga gagcgtcaag
      241 gtggcgctgt cgggggacgg cggcgacgag ctgttcgccg gatacgacca ctacaaggcg
      301 gagcgtctcc tggaagggta cgcccgcatc ccggcgcccg tgcgccgcgg cgccctcacg
      361 ctgctcggac gcgacgccga gcgccgcccg acgaagcgcg ggggcgtgcg gcggcgcctg
      421 cgccgcctcg aggaggcgct cgcccagccg gccttcctcg cgcaggcccg cttcatggtg
      481 cgcagccgcg acgaaatgcg ggaggggctc ctgccggatc ccggccccga cgaccccgcg
      541 gcgggatggg cggagccgtt cgcggcggtg gtgcgtgaca gccccttccc gcccggcctg
      601 gcgcaccagc agttcatcga cctgaagacg ttcctggcgg aggacatcct gttcaagacc
      661 gaccgggcga gcatggcggt gtcgctcgag gcgcgcgtgc cgtacctcga ccacgagctc
      721 gtggagttcg ccttccggct ggcaccgtcg ctgaagctgc gcggcctggt cggcaagtgg
      781 atcctgcggc gcgccttccg cgatcgcctc ccggcgtcga tcctcaggcg ccgcaagagc
      841 ggattctcgg tgccgatcgc cgcctggctg cgccatgatc tgaagacgct ggcgcgcgac
      901 ctgctcgcgc cgggacgggt gacgcggcag gggctcttcg acgcccggag ggtctccgcg
      961 gttctcgagg agcacctgga cgggcgcgcc gatcacgcgc ggcttctgtg ggccctggtg
     1021 atgttccaac tgtggcacga gcgctggtcg gcggccgcgc cggccgccgc ggtggtgccg
     1081 atcgcggcgg acgaaggggt ggcctgatgc aggacgatcg gcttctcaat ctctcgtatt
     1141 ggaccgagct ggcgtcgcgg cgccgctggc tgatcctcgg gtgcacggcg ggggtgttcg
     1201 tgtcggccac cgccttcagc ctcatgcgcc ccaatctgta cgaggcgacg agcacgctcc
     1261 tgctcagccg ccagcgcgtg caggccttcg acgcgcagga cctctacacc aagcagcagg
     1321 ccgggacccg ccccaacgag gtggtggcgg cacagatcga aatcctcaag agcatgccgg
     1381 tcctggagcg ggcggtgcac gatctcgagg agcgggacct gctgcacttc gaccggcccg
     1441 acccggggga caagccgcca ctcgtgagcc gcgtcctcat cgccttgcat ctccgcgctc
     1501 cggagccgcc gccgaccccc gacgagcgtc gtcgcggcta cgtgcgcgac ctgaaggacc
     1561 ggatgtcgac cggcactcgc ggcggcaacg ccttcctcac cgtctcggtg cagagccgca
     1621 acccggagat ggcggcggag accgccaacg ccatcaccaa cgcgtacctc aggcacaccc
     1681 gcgagctgat gcggcgcagc gccgacgatg ccgtcgagtg gctctccggg aagctgcgcg
     1741 agcagcggga caaggtgctg gagaccgagg agcggctgcg gtccctctcg ggccccgcgc
     1801 cgcaggcgga ggacatggat cggctgacgg tccaggagat gagccgcatc caggcggcgc
     1861 tgctcgacgt ccgactgatg atgctccggg cggagaccgg cggccccgtg gcgggctcgg
     1921 gcgcggcggc gccccccgcg aacaacagcg tcgacgacgc ggtcaacggc gcgctccggg
     1981 acaaggtgca gcaggagctg gtcgagacgg tctcgacgct cagcaggctg cgccgcacct
     2041 acggcgagaa ccaccccgac gtcatcgccg cggccgagaa ggagcagcag ctgcgcgccg
     2101 agctggagcg gctgaacacg ctggcgccgc ggccgcggtc gaacgacccg atgggtcgca
     2161 tcactccctc cgacgcccag gcgctcaagg cgcaggagcg cctgctgcag cagcagctga
     2221 acatcaccct gcagtccagc tcggcgaaag gggaggccgc gatgcactac gcgatcctca
     2281 agaaggaggt ggagatcaac cggtcgctct acaacgagat gctgacccgg ttgaacgaga
     2341 tcaccatcgc ctcggggctc gatcccggat ccgccgagac cttcgagccc gcccgcatcc
     2401 cgcgcgcccc cttctcgcca cagcacacgc agacgatcct gctcgggttc gccgcggggc
     2461 tcctgctcgg tctggcgggg gcggcgttgc gcgatcacat ggatcagtcg ctgcgcgatc
     2521 ccgggcaggc gaacgacctg ctgcgcgcgc cggtcctggg gatcatcccg gaccaccgct
     2581 ccctcgtcgg ccgcccggag ccgcgccggg gaacccggat gtgggtcggc cacggcgagg
     2641 agtcggtgag cgccgaggcg taccgggtgc tgcgctcgca catcgagggg acgctgaccg
     2701 cggaggaatc ccgcatcctc atactgacga gcgcggtggc gggggagggg aagtcgacca
     2761 ccgccgccaa tctcgcggcg gctttcgcgg aatcggggcg ccgggtcctg ctgatcgatg
     2821 ccgacttccg ccgcccgacg ctgcgcaagg tgctcggggt ggagctcccc gaggatcacc
     2881 agctgtcgct cgtcctcagc ggatcgaagc cccctgagaa ggtcatcctc gactcgggga
     2941 tcccggacct cgacttcatc gggcaccgcg ccaacggcaa gatccccgat ggcagcaaga
     3001 cgaccgagtc gttcaagaag ctcttcgcct ggggacgcga ccgctacgac cggatcatcg
     3061 tcgacctgcc ggtcctgatg atcgcgccgg gggtcaccga gctgggacgc gccggtggca
     3121 cggtgctcct ggtgcaccgc cccggctggg tgcccgcccc ggtcctcgaa tccgtgcgtc
     3181 agcacctggc gatggccaag acccggctgg tcggggcgat cctgaacggc accggcgccc
     3241 gctgggcttc atcgcagtac cttccggtct actaccacgc cgcctactac tcgcggaaga
     3301 tcgaggcccc ctgaggagcc cggcggaatc gcgcgtgtcg tcccgccgcg ggcggctctg
     3361 gccggtgatc gcccgggtgg cggcgtggag cggactcggt ccggcggcgg gcgtcctgtg
     3421 gcgccgccgc gccgccggga cctggacgat cctcggctac caccgggtcg gcgaaccctc
     3481 ggatgacggc ggtccggacg ccgtccggcc ggagcgcttc cgcgaccatc tgagatggct
     3541 cagggcgcgc tacgacgtca tgacggtcgg ggaggggctc gctgcgctgc gccggggcgg
     3601 agcgcggcgg ccgatggtga gcctcacgtt cgacgacggc taccgggaca acgtcgtcca
     3661 cgccctgccg atcctcctcg cggagggatg ccgcgccacg ctctacccga cgctcgaggc
     3721 gatcgaggag gggcgggcgc cgtggccgca ccggctggcg ctggacctgc ggcgcctcac
     3781 tcgccggggc gacgcgaggc ccgactcctg gccggagccg ctcgccgctc tggccggggc
     3841 cgccgggctg ggcccgcggg aggtgtccga gcgcatcgac cggatcgtgt cggaggcgaa
     3901 gcgctggccg tccgaggaat gctcccgcat cacggcggag gtcgagcggc tcgccggccc
     3961 cgcggcgtcg cggccggaga
//
LOCUS       DASRIQ010000306         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1687655, whole genome shotgun sequence.
ACCESSION   DASRIQ010000306 DASRIQ010000000
VERSION     DASRIQ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687655"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VFD06_07380"
     CDS             <1..1184
                     /locus_tag="VFD06_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN03396.1"
                     /translation="GRRLRGVLVSAQVALSLVLLIGAGLQVRSLMRLLHVDPGFDTSN
                     ALMFEVSLPDRRYPDSAAQVAFFEKAVEAVRALPGVESAAVVTAVPLSDSQLIFSLEE
                     IEGKPPAAPGDNQSANWYAVTPDYFKTLRLPLQRGRVFTPEDRAGAPRVMLINEAFAR
                     RMFPGEDPIGRRVRIGVDSDAKREIVGIVGDVRHYGLDQEATMQMYEPLAQRPWDTAS
                     LVLRTTVPASTLSQPARQAILSIDREQPVSTAATLDELVARSTGQRRFSLLLVSVFAV
                     TALFLTAIGLYGVVSYFVSLRTHEIGVRLALGATRREILTLVVGQGMRLALAGVAVGL
                     AAAFAVTRLLTGLLFGVSATDPITFAATAAGVAAVTLFASAFPAWRAARVSPSAALRC
                     E"
     gene            1192..>2941
                     /locus_tag="VFD06_07385"
     CDS             1192..>2941
                     /locus_tag="VFD06_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HZN03397.1"
                     /translation="METVIQDVKYGVRMLLKSPGLTAVAALSLALGIGANTTIYTLIN
                     MLFLRPIAIEDAHNVVAVYTTDEKNKSQFEVSQTSYPNFKDYRERNQVFSGLAAHQFV
                     GLNITGAGEPESTLGEMVDSNYFELLGVRAAQGRVFLAEEGKVDGGAPVAVLSHGYWT
                     RRFGADPATVGRTLSLNGHPFTVIGVLPADFGGLVAFGGPDLFVPMAMHKELLTGFMA
                     ENFEDRRALTWDIVARLKPGTTPEQARADLRRIGAQLEKEYPEPNGQRNATVLPIAQA
                     LINPGIRNVFKMSAWLLMTVVALVLLIACANVANLLLARSGARRKEIAIRLSLGANRA
                     RLVRQLLTESVMLSLLGGLLGLLLAYWSRDLLVASRPAQLFPNGIDVPLDARVFLFTL
                     AISVATGLLFGLAPALQSSRSDLAIELKDRTQEPASRRKVSLRGGLVVAQVALSLVAL
                     IGAGLFVRSLRNTQAIDPGFEPKNLLVMTFDVGAQGYDQARGEAFYRDVAERARALPG
                     VRSVSLSANLPIAGGGFSRTVFPEGRDQSTGSTGTFVLCDTIAPGYLPTLDIALLKGR
                     CRASGGSRGRWCRTGRR"
BASE COUNT          404 a         1058 c         1012 g          467 t
ORIGIN      
        1 cggggcgccg gctgcgcggc gtgctggtgt cggcgcaggt agcgctgtcg ctcgttctgc
       61 tcatcggagc cgggctccag gtgcgcagcc tgatgcgcct gctccacgtc gatcccggct
      121 tcgacacctc aaacgccctg atgttcgagg tcagccttcc ggatcggcgc tatccggatt
      181 cggcggcgca agtcgccttc ttcgagaaag cggtcgaggc ggtgcgcgcc cttcccggcg
      241 tcgagagcgc cgccgtggta accgccgtgc ctctctccga ttcgcagctc atcttctcgc
      301 tcgaggagat cgaggggaag ccgccggccg cccccggcga caaccagtcc gcaaactggt
      361 acgcggtcac tccggactac ttcaagacgc tgcgcctgcc gctccagcgc ggccgggtgt
      421 tcacgccgga ggaccgggcg ggggcgccgc gcgtcatgct gatcaatgaa gcgttcgccc
      481 gccgcatgtt ccccggcgag gacccgatcg gccggcgcgt gcgcatcggg gtggacagcg
      541 acgcgaagcg ggagatcgtc ggcatcgtcg gcgacgtccg gcactacggc ctcgaccagg
      601 aagcgacgat gcagatgtac gagccgctgg cgcagcgtcc gtgggacacg gcgagcctgg
      661 tgctccgcac cacggtcccg gcgtccaccc tgtcgcagcc ggcgcgccag gcgatcctgt
      721 cgatcgatcg ggagcagccg gtctccacgg cggcgaccct cgacgagctg gtcgcccgat
      781 cgacggggca gcggcgcttc agcctgctgc tcgtgagcgt cttcgcggtg accgccctct
      841 tcctgaccgc catcggtctc tatggcgtgg tctcctactt cgtgtcgctg cggacccacg
      901 agatcggcgt ccggcttgcg ctcggcgcca cccgccgcga gatcctgacg ctggtggtgg
      961 gccaggggat gcggctggcg ctcgccggcg tcgcggtcgg gctcgccgcc gccttcgccg
     1021 tgacccggct cctcacggga ctgctcttcg gggtcagcgc caccgatccc atcaccttcg
     1081 cggccacggc ggcgggtgtc gccgccgtga cccttttcgc cagcgccttc cccgcgtggc
     1141 gggccgcacg cgtctccccg agcgccgcgc tgcgctgcga gtgaggttcc catggaaacc
     1201 gtgatccagg acgtgaagta cggggtgcgg atgctcctca agagccccgg cctgacggcc
     1261 gtggcggcgc tgtcgctggc gctcggcatc ggcgccaaca cgacgatcta cacgctcatc
     1321 aacatgctgt tcctgcggcc gatcgccatc gaggacgccc acaatgtcgt ggcggtctac
     1381 accaccgacg agaagaacaa gagccagttc gaggtgagcc agacctccta cccgaacttc
     1441 aaggactacc gggagcgcaa ccaggtcttc agcggcctgg cggcgcacca gttcgtcgga
     1501 ctcaacatca ccggggccgg cgagccggaa tcaaccttgg gcgaaatggt cgactccaac
     1561 tacttcgagc tgctgggcgt gcgggcggcg cagggccggg tgttcctcgc cgaggagggg
     1621 aaggtggacg gcggagcgcc ggtggcggtg ctgagccacg gctactggac gcggcgcttc
     1681 ggggcggacc cggcgaccgt cgggaggacg ctcagcctca acggccaccc gttcaccgtc
     1741 atcggcgtgc tgccggccga cttcggcggt ctcgtcgcct tcggcggtcc ggacctgttc
     1801 gtgccgatgg cgatgcacaa ggagctcctg accggcttca tggccgagaa cttcgaggac
     1861 cggcgggcgc tgacctggga catcgtcgcg cgcctgaagc cgggaaccac cccggagcag
     1921 gcgcgcgccg acctgcgccg catcggcgcc cagctcgaga aggagtaccc ggagccgaac
     1981 gggcagcgca acgccaccgt cctgccgatc gcccaggcgc tcatcaaccc cgggatccgc
     2041 aacgtcttca agatgtcggc gtggctcctg atgacggtcg tggcgctcgt gctgctgatc
     2101 gcgtgcgcca acgtcgcgaa cctcctgctc gcccgctccg gcgcgcggcg caaggagatc
     2161 gccatccggc tgtcgctcgg ggcgaaccgg gcgcgcctgg tccggcagct cctgaccgag
     2221 agcgtcatgc tgtcgctcct gggcggactg ctcggactgc tcctggccta ctggagccgc
     2281 gacctgctgg tggccagccg gccggcgcag ctcttcccga acggcatcga cgtgccgctc
     2341 gacgcgcgcg ttttcctgtt cacgctcgcc atttccgtcg ccaccgggct cctgttcggc
     2401 ctagcgccgg cgctgcagtc gtcccgttcg gacctcgcga ttgagctgaa agaccggacg
     2461 caggagccgg cctcgcgccg caaggtgtcg ctgcgcggcg ggctcgtcgt cgcgcaggtc
     2521 gcgctgtcgc tcgtcgccct catcggcgcg gggctgttcg tccgctcact ccggaacacg
     2581 caggcgatcg accccggctt cgagccgaag aatctcctgg tgatgacctt cgacgtcggg
     2641 gcgcaggggt acgaccaggc gcgcggcgag gcgttctatc gcgacgtcgc cgagcgggcc
     2701 cgggcgcttc ccggcgtccg gtcggtgtcg ctcagcgcca acctgccgat cgccggcggc
     2761 ggcttcagcc gcaccgtctt ccccgaaggg cgcgaccagt ccaccggttc caccggcacc
     2821 ttcgtcctgt gcgacaccat cgcccccggc tacctcccga cgctcgacat cgccctgctg
     2881 aaagggcgat gtcgagcgtc gggaggtagc cgggggcgat ggtgtcgcac aggacgaagg
     2941 t
//
LOCUS       DASRIQ010000307         7572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2195816, whole genome shotgun sequence.
ACCESSION   DASRIQ010000307 DASRIQ010000000
VERSION     DASRIQ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195816"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VFD06_07390"
     CDS             <1..1013
                     /locus_tag="VFD06_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HZN03398.1"
                     /translation="WAAVEVALFSLGLLLLRRGGARGAATSWGLPAAAAISLLVAAAP
                     ARTMAPRASLTTPSPIRDLVDRGQGATRLHHSPRPPGLAVRGETDEQIWGYRFDRFTY
                     ALQTGHPDEVPTVFDAATDRMDLAAGAALAARLPALPAADQVRILRVASAGTVLAYAP
                     LDDPGLVPGPTLAGASRPEARLYRVADPLPRVRFVSRQRPPRDPGDAAGSLLDPRFEP
                     DSEVLLEGAVRSDPPGPPDRAEIEVLQDDPERVTIRLVAPRAGHLVVADSDAPGWVAR
                     VDGAPAPILRANMLFRAVPVPAGRHTIEMRYRPRSVFAGALASFAGAITLGTLCARRR
                     RR"
     gene            1010..2800
                     /locus_tag="VFD06_07395"
     CDS             1010..2800
                     /locus_tag="VFD06_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03399.1"
                     /translation="MSGAPERSGGGDRLLLGILLLLLAAATTLSPIRNYDYWWHLKTG
                     ALILEEGRVPHGDPYSFTSAGTPWVDHEWLFQVLAYAGHMAFGPALLVMIKSALLCGL
                     VLLAARFLLREGHGPAGSAVLLMLALVGGAFRFDVRPELATLLIVPLSVDLAMRARRH
                     RTLWPLAVIVALTALGANLHVGVLLVPALLWAGTAATLLSSWLGRLAIGRSGTGEDHP
                     AGFAGRLALTAAAATLAAGANPYGLRIYVVPFELRRLLQGLPWPNLEWVAPTWETLPL
                     LFVAAGLAAVVLIAGWRAADPIAAPALLLCLAIALAHVRNAGLFFLLLPWGLARPARY
                     LADVIKRNAMYRRGTGGERVRPGFILAVVLLASGLPLLLWLPPEPRFGAGIDAGNEPR
                     RAVDFLDAERIGGPLYNDVRFGGYLIWRRFPVDRVFIDSRNEIYGDLMRDIAVSMKGP
                     DAWRGFLDRHRIDAAFLRYNPTLQKVVYTAPDGSQRTGERAFSATYFPSTAWALVYWD
                     DDVMVMLRRTGRHADTIARLEYRALQPDDWRFLWASALIGRIPGGPILAEIQRKLRED
                     PDCARARELREWFAPFAGAPEEATLSRRGR"
     gene            2989..3441
                     /locus_tag="VFD06_07400"
     CDS             2989..3441
                     /locus_tag="VFD06_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN03400.1"
                     /translation="MKKQKGFTLIELLIVVAIIGIIAAIAIPNLLNAIDRGKQKRTMA
                     DMRSIGTAIESYAVDNNFYPKGMANAASAAIGAHVSPIYIKTMPTTDGWSNVWDCDST
                     NTGAQYTITSSAKDGVQGTNNGGTTSVFESDIVFANGQFFQWPQGTQT"
     gene            3512..5149
                     /locus_tag="VFD06_07405"
     CDS             3512..5149
                     /locus_tag="VFD06_07405"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN03401.1"
                     /translation="MSVAPLLAAALLAVAVLLPPEIHPAGRLAAHLLGAATALALAWG
                     GWPLSPRARWCGLLLLPLPVASLLLASGGRARGLEEAAAFAAFGLAVLLGRRVAARGE
                     PGRIPFPAVLAVLGTLAAAQAILQHHWAYPRAAAELRAAGPAEAEAYLGRLEQGRPAG
                     PFSLPAALGGFLALALPMTLAVRRQAAGAAVRWGVTAALLTQGYALFLTRSLGGLLAA
                     TVALGIAASSSRPGRPWREAVAAAIAFAAIAGFFIHGRRAEIGARPGGDPVTLRLGNW
                     EAAARMISGHPFLGVGPGRFPVFYPRFMRPGMNETQYAHNSYLQVAATWGAWTVLPLG
                     LLAAAALRRRAARDGAGPAAYAAGAGFLVHNAVDFTFFLPGVALPAGLLLGFSSPPAQ
                     EETGAPASRHRGRDRMAGVLLAAAFLAYAATADRAVRHFDHARTLAVAGKPEEAERRA
                     LRAVSAWPLDPDPWAFVAQSLLARGAADPASVALLAEAAERAVTLEPEAAILHHTRAL
                     DRAAAADPAAAWIEEQRAAQLFPEKELYRSSLQDAGR"
     gene            5146..7089
                     /locus_tag="VFD06_07410"
     CDS             5146..7089
                     /locus_tag="VFD06_07410"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN03402.1"
                     /translation="MSRLLLGCAAILLFLAPFGEGGRRPDILAALHLVALGLGLIAAI
                     SLAGSPDGGRLRGRGNGLLVLVGAALAWSCAAATAAPFREAALFGLMDRLATAALFIA
                     GAILFHDETSLVRLRTIAVAATTLQAAIALAGTLVSGPAGGAHLFLNRSQLAAYLGIG
                     FFLAASAALGAWRRGDRRSTAIASGAALVHILAILPLQSRGALLGLACGGLGFLAASW
                     TALPRRARAALVAGGAVVAICGSVLVAKRFRDSDDPDRYTRVPIWRASLAMASERPLR
                     GLGPGQFPHEAPKHNFPLERSPVRYARQFSGAHSLPLTLFAEEGWPGLLLAAAIASAI
                     LRTSLRSGRHAPGGGTPARDAILGVGAAVLALSAQGMVEDLQERPAILLTAALLAGSA
                     CAAARGWRPERCRVDPIAAGALGLLAACVAAGGVVLPWLAWREAQAARALGPVGLPRL
                     ERAAQLDPLNPWHREGLAMAALQGGPPDRQRYALAAIHLDAARRLAPREARLALLRAR
                     LEAVAARSLFPVDATIDRAASLYDQAVALSPRDPRPRLEQAGWLAEQGRRQEARATLE
                     QALAIEPNYRRARILLTSLVEREGDYEEARRAYDALLASDAALDDYVPDSGYAAEIVR
                     DAPEERDRVAAWFRPRSHCLDES"
     gene            7191..>7572
                     /locus_tag="VFD06_07415"
     CDS             7191..>7572
                     /locus_tag="VFD06_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HZN03403.1"
                     /translation="MCGIAGVFEFGRESGRVDEALLTRMRDTLTHRGPDDAGLYLSPD
                     RTLGLAHRRLSIIDLSPAGRQPMSNEDGTVWITFNGEIYNHLDLRRELEAKGHTYRSR
                     TDTETLVHLYEEDGEDFVRRIEGDF"
BASE COUNT          902 a         2799 c         2686 g         1185 t
ORIGIN      
        1 tgtgggcggc ggtcgaggtc gcgctgttct cgctcgggtt gctcctgctg cgccgcggcg
       61 gcgcgcgggg ggccgcgacc tcctggggtc tgccggcggc cgcggcgatc tccctcctcg
      121 tggcggctgc gccggcccgc acgatggcgc cgcgcgccag cctcacgaca ccgtcgccga
      181 tccgcgacct cgtcgaccgc ggccagggcg ccacgcggct ccaccactct ccgcggccgc
      241 cggggctcgc cgtgcgcggc gagaccgacg agcagatctg gggctaccgc ttcgatcggt
      301 tcacctacgc gctccagacc ggccacccgg acgaggtgcc gaccgtcttc gacgcggcca
      361 ccgaccggat ggatctcgcc gcgggggccg cgctcgccgc gcgccttccg gcgctgccgg
      421 ccgcggacca ggtccgcatc ctgagggtgg catccgccgg aacggtcctg gcctacgcgc
      481 cgctcgacga tccggggttg gtccccggac cgacgctcgc cggcgccagc cgtccggaag
      541 cgcgcctcta tcgcgtcgcc gatccgctgc cgcgcgtccg cttcgtgtca cgccagcggc
      601 cgccgcgcga tcccggcgac gcggccggaa gcctcctcga tccccggttc gaaccggaca
      661 gcgaggtgct gctcgagggc gcggtgcgca gcgacccgcc cggtccgccc gatcgggcgg
      721 agatcgaagt cttgcaggac gatccggagc gcgtcacaat caggctcgtg gctccgcgcg
      781 ccgggcatct cgtggtcgct gacagcgacg ccccgggctg ggtggcgcgt gtggacggcg
      841 cccccgcccc catcctgcgc gccaacatgc tcttccgcgc cgtgccggtc ccggccgggc
      901 gccacacgat cgagatgcgc taccgtccgc gcagtgtttt cgccggcgcc ctggccagct
      961 tcgccggcgc catcacgctc gggacgctct gcgcgcggcg gcgccggcga tgagcggcgc
     1021 gccggagcgg tccggcggcg gggaccgcct gctcctgggg atcctgctcc tgctcctggc
     1081 cgccgccacc accctcagcc cgatccgcaa ttacgactac tggtggcacc tcaagaccgg
     1141 cgctttgatc ctcgaggagg ggcgggtccc gcacggcgat ccctattcgt tcacatccgc
     1201 cgggaccccc tgggtcgatc acgagtggct gttccaggtg ctggcctacg cgggacacat
     1261 ggcgttcgga ccggcgctgc tggtgatgat caagagcgcc cttctctgcg gcctcgtgct
     1321 gctcgcggca cgattcctcc tgcgcgaagg acatggcccc gccggatcgg cggtgctgct
     1381 catgctggcg ctcgtcggcg gcgccttccg gttcgacgtg cggccggagc tcgccaccct
     1441 gctcatcgtg cctctgtcgg tggatctcgc gatgcgcgcc cggcgccatc ggaccctctg
     1501 gcccctcgcg gtgatcgtcg ccctgacggc gctgggcgcc aacctgcacg tgggtgtcct
     1561 gctcgtcccg gcgctcttgt gggccggaac cgcggcgacg ctcctgtcct cgtggctcgg
     1621 gcgactcgcg atcggtcggt ccgggacggg agaggatcat ccggcgggat tcgccggccg
     1681 gttggcgctg accgcggccg cggcgactct cgccgccggc gccaatccgt acggcctgag
     1741 gatctacgtt gtgccgttcg aactgcggcg gctgctccag gggctgccgt ggccgaacct
     1801 cgagtgggtc gccccgacct gggagaccct gccgctgctc ttcgtcgccg cggggctcgc
     1861 cgccgtcgtc ctgatcgcgg ggtggcgggc cgccgatccg atcgccgccc ccgcgctcct
     1921 cctgtgcctg gcgatcgccc tggcacacgt gcgcaacgcc gggttgttct tcctgctcct
     1981 tccctggggt ctggcgcggc cggcgcgcta cctcgcggac gtgatcaaga ggaacgcgat
     2041 gtaccgccgg gggacgggag gggagcgcgt ccgcccgggg ttcatcctcg ccgtggtcct
     2101 gcttgcgagc gggctgccgc tcctgctctg gctgccgccg gagccgcggt tcggagccgg
     2161 catcgacgcg ggcaacgagc cgcgccgcgc cgtcgatttc ctcgacgcgg agcggattgg
     2221 cggccccctc tacaacgacg tgcgcttcgg cggttatctc atctggcggc gcttccccgt
     2281 cgatcgggtg ttcatcgaca gccgcaacga gatctacggc gatctgatgc gtgacatcgc
     2341 cgtctcgatg aaggggccgg acgcgtggag aggcttcctc gaccgccacc ggatcgacgc
     2401 cgccttcctg cgctacaacc cgaccctcca gaaggtggtc tacacggccc ccgacggatc
     2461 gcagcggacc ggcgagcggg ccttctccgc aacttacttc ccatcgacag cctgggccct
     2521 ggtgtactgg gacgacgatg tcatggtgat gctccgccgc accgggcggc acgcggacac
     2581 catcgcaagg ctcgaatacc gcgccctgca gccggacgac tggcggttcc tgtgggcctc
     2641 ggcgctcatc ggccggatcc cgggcgggcc gatcctggct gagattcagc gcaagctgcg
     2701 cgaggacccg gactgcgccc gggcgcgcga gctcagggag tggttcgctc ccttcgccgg
     2761 cgctcccgaa gaggcgacgt tgtcacggcg tggacggtga cgctggttgt caccctcccc
     2821 cccgggaaac ctgacgaatc ttgctggtcg cggctgctgc cgccgatgac ggaatcgaaa
     2881 gtgagcggcc aagggcccgt ggatcaaggg tttggaggag ggcggtgaag cgcggacgac
     2941 gtggcatcgg ggttgctctt tcggagttgc cgaaaggggg acccacagat gaagaaacag
     3001 aagggcttca cgctaatcga gctgctcatc gtcgtcgcga tcatcggcat catcgccgcg
     3061 atcgcgatcc cgaacctgct gaacgccatc gaccgcggca aacagaagcg gaccatggcc
     3121 gacatgcgct ccatcggcac ggcgatcgag tcgtacgccg tggacaacaa cttctacccg
     3181 aagggcatgg cgaacgccgc ctcggctgcc atcggcgcgc acgtgtcgcc gatctacatc
     3241 aagaccatgc cgacgaccga cggttggtcg aacgtctggg actgcgactc gacgaacacc
     3301 ggcgcgcagt acaccatcac gtcgtccgcc aaggacggcg tgcagggcac gaacaacggt
     3361 ggaacgacct cggtcttcga gagcgacatc gtgttcgcca acggccagtt cttccagtgg
     3421 ccgcagggaa cccagaccta gtcgttcgtt cgaactcgaa catcggggcg gtgccttcgg
     3481 gcaccgcccc tttgcttttt ggggtcacgg cgtgagcgtc gcaccgctgc tcgccgccgc
     3541 cctcctcgct gtcgcagtcc tccttccgcc ggaaatccac cccgccggcc gactcgcggc
     3601 gcacctcctc ggcgccgcca cggccctggc gctggcgtgg ggcggctggc ccctgtcgcc
     3661 tcgcgcccgg tggtgcggct tgctcctcct gccgctgccg gtcgcctcgc tcctcctggc
     3721 gtcgggagga cgggcgcgcg gtctcgagga ggcggccgcc ttcgcggcgt tcgggctcgc
     3781 cgtccttctg ggacgccggg tggcggcccg cggggaaccg ggacggattc cgttccccgc
     3841 cgtgctcgcg gtcctcggca ccctcgcggc ggcccaggcc atcctgcagc accactgggc
     3901 ctatccgcgc gccgcggcgg agctgcgcgc cgcgggcccg gccgaggcgg aggcgtacct
     3961 cgggcgcctc gagcagggcc ggccggccgg gccgttcagc ctgccggcgg cgctcggcgg
     4021 cttcctcgct ctggcgctgc cgatgacgct cgccgtccgg cggcaggccg cgggggccgc
     4081 ggttcggtgg ggggtgacgg cagcgcttct gacgcagggg tatgcgctct ttctgacccg
     4141 atcgctcgga ggccttctgg ccgccaccgt ggcgctcggg atcgcggcgt cgtcgtcgcg
     4201 gccgggccgg ccgtggcgcg aggcggtcgc cgcggcgatc gccttcgccg ccatcgcggg
     4261 attcttcatc cacggccggc gcgccgagat cggtgcccgt cccggaggag atccggtcac
     4321 cctgcgcctt ggcaactggg aggcggcggc gaggatgatc tccgggcatc cgttcctcgg
     4381 cgtcgggccg ggacggttcc cggtcttcta tccgcgcttc atgcgccccg ggatgaacga
     4441 gacgcagtac gcgcacaact cgtacctcca ggtcgccgcg acgtggggag cctggaccgt
     4501 cctgccgctc ggactgctcg ccgcggcggc gctgcgccgc cgcgcggcgc gggacggcgc
     4561 ggggccggcc gcctatgccg ctggggccgg gttcctcgtg cacaacgccg tcgacttcac
     4621 cttcttcctt cccggggtcg cgctcccggc cggactcctt ctcggattct cctccccccc
     4681 tgcgcaggag gagaccggcg cgccggcgtc gcgacaccgc gggcgcgatc ggatggccgg
     4741 cgtgcttctc gccgcggcat tcttggcgta cgccgcgacc gcggatcgcg ccgtccggca
     4801 tttcgatcac gcccgcacac tggcggtcgc ggggaagccg gaagaggcgg agcgacgggc
     4861 gctcagggcg gtctcggcat ggcctctcga tcccgatccg tgggccttcg tggcacagag
     4921 cctcctcgcc cgcggggccg ccgatcccgc gtcggtggcg ctcttggccg aagcggcgga
     4981 gcgggccgtc accctggagc cggaggcggc gatcctgcat cacacgcggg cgctcgaccg
     5041 cgccgccgcc gccgatccgg cggcggcctg gatcgaggag cagcgcgccg cgcagctctt
     5101 cccggagaag gagctgtacc gctcgagcct tcaggacgca ggccggtgag ccgcctcctg
     5161 ctcgggtgcg ccgcgatcct gctcttcctg gcccctttcg gggagggggg acgacgcccc
     5221 gacatcctgg cggcgctgca cctggtggcg ctgggcctcg gcctcatcgc cgccatctct
     5281 cttgccggat ccccggatgg cgggcggctg cgcggtcggg ggaacgggct cctggtgctc
     5341 gtcggtgcgg cgctcgcctg gtcgtgcgcc gccgccacgg ccgctccgtt ccgcgaggcc
     5401 gccctcttcg gcctgatgga tcggctggcg accgccgcgc tgttcatcgc cggggcgatc
     5461 ctgttccacg acgagacgtc gctcgtgcgc ctgagaacga tcgcggtcgc ggcgaccacg
     5521 ctgcaggcgg cgatcgccct cgccggcacg ctcgtctccg gccccgccgg cggcgcccac
     5581 cttttcctca accgcagcca gctcgccgcc tatctcggca tcggtttctt tctcgccgcg
     5641 tccgcggctc tcggcgcatg gaggcgcgga gacaggcgat ccacggcgat cgcgtcgggt
     5701 gccgcgctcg tgcacattct cgccatcctg ccgctccagt cccgcggcgc gctcctggga
     5761 ctcgcgtgcg gaggcctggg attcctcgcc gcctcgtgga cggccctgcc gcgccgcgcg
     5821 cgggccgcgc tcgttgccgg cggcgcggtc gtggcgatct gcgggagtgt cctcgtcgcg
     5881 aagcgtttcc gcgacagcga cgatcccgac cgctacaccc gcgtcccgat ctggcgcgcc
     5941 tcgctcgcga tggcgtcgga gcggccgctt cgcggcctcg gccccggcca gttcccgcat
     6001 gaagcgccga agcacaactt cccgctcgag cgcagccccg tacgctacgc ccgccagttc
     6061 tccggggcgc actcgctgcc gctgacgctt ttcgccgagg agggatggcc ggggctcctg
     6121 ctcgcggccg ccatcgcgtc cgcgatcctc aggacttcgc tccgttccgg ccggcacgcg
     6181 cccgggggcg gcacccccgc gagggatgcg atcctcggcg tgggggcggc cgtcctggcc
     6241 ctctctgcgc aggggatggt cgaggatctg caggaacgcc cggcgatctt gctgaccgcg
     6301 gcgcttctcg ccggatcggc ctgcgccgcg gcgcgcggat ggcggccgga gcggtgtcga
     6361 gtcgatccga tcgcagccgg ggcgctcggg ctcctggcgg cgtgcgtcgc ggcgggcggg
     6421 gtggtcctcc catggctcgc gtggcgcgaa gcccaggcgg cgcgcgcact cgggccggtg
     6481 gggctgccgc ggctggagag ggccgcgcag ctcgatcccc tcaatccgtg gcatcgcgaa
     6541 gggctggcca tggcagcgct ccagggaggg ccgcccgacc ggcagcggta cgctttggca
     6601 gccattcacc tggacgcggc gcgccgtctt gcgccccgcg aggcgagact ggcgctcctg
     6661 cgcgcccgtc tcgaggcggt ggcggcccgc tcgctgttcc cggtcgatgc gacgatcgac
     6721 cgcgcggcgt cgttgtacga ccaggcggtg gcgctgtcgc cgcgcgatcc gcggccgcgg
     6781 ctggagcagg ccggctggct cgccgagcag gggcggcggc aggaagcgcg cgccaccctc
     6841 gagcaggcgc tcgcgatcga gccgaattac cgccgagccc gtatcctgct cacctcactc
     6901 gtcgagagag agggggatta cgaggaggcg cgacgcgcct atgacgctct tctggcgagc
     6961 gacgcggcgc tcgatgacta cgttccggac agcggttacg cggccgagat cgtccgcgac
     7021 gcccctgagg agcgggaccg ggtcgccgcc tggttcaggc cccgaagtca ttgcctggac
     7081 gagagttagc tttaccgtca atctctggac aaggggcctt ccggacatta tattgacgcg
     7141 gcgcaagtcc ttattgcgct ttgagataga cgggaggggt cgcctcagcc atgtgcggca
     7201 tcgcaggggt cttcgagttc ggccgggagt cgggacgggt cgatgaagcg ctcctcacgc
     7261 ggatgcgcga cacgctgacc catcgcggcc cggacgacgc cgggctctac ctcagcccgg
     7321 atcgcaccct cggcctcgcg caccggcgcc tgtcgatcat cgatttgagt ccggccggcc
     7381 ggcagccgat gtccaacgag gacgggaccg tctggatcac cttcaacggc gagatctaca
     7441 accatctcga cctgcgccgc gagctcgagg cgaaagggca cacctatcgc tcccgcaccg
     7501 acaccgagac cctcgtccac ctctacgagg aggacgggga ggacttcgtc cgccgcatcg
     7561 aaggggactt cg
//
LOCUS       DASRIQ010000308         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1180352, whole genome shotgun sequence.
ACCESSION   DASRIQ010000308 DASRIQ010000000
VERSION     DASRIQ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180352"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="fabG"
                     /locus_tag="VFD06_07420"
     CDS             complement(<1..713)
                     /gene="fabG"
                     /locus_tag="VFD06_07420"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZN03404.1"
                     /translation="MSAAELQGKVSLVTGASRGIGRAIALELARRGARVIVAARDRDR
                     LEATAAACREAGGQADAVTLDLADAASVKAGIDAALKGAGGRIDHLVNNAGVTGDGLL
                     LRMKREEWDRVLLTNLTGAFETTRLVLPAMLKARSGRIVNISSIVALMGNPGQANYCA
                     AKAGLIGLTKSLAREVASRSITVNAVAPGLIDTDMTKDLPEAARTKMADSIPLQRLGT
                     AEDVAQAVAFLVGPGGAYIT"
     gene            complement(710..1675)
                     /gene="fabD"
                     /locus_tag="VFD06_07425"
     CDS             complement(710..1675)
                     /gene="fabD"
                     /locus_tag="VFD06_07425"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZN03405.1"
                     /translation="MSGARKIALVFPGQGSQVVGMGRAAHEAFPEVRAVFDAADRALG
                     FPLSAACFDGPEEALRMTATTQPAILTTSVALLEAMRSRLPGLDARVACAAGHSLGEY
                     SALVAAGALALEDAARVVRRRGEYMQEAVPVGQGAMAAVLGAPLDAVEAMCREAAEGE
                     VLSPANLNAPDQTVVAGHAGAVRRLPPVAKKLGARRVVELPVSAPFHCALMRPAQERL
                     TRDLAALPLQDARFPVLPNVSAEPERTAAGFRKALEEQVTAPVRWVETVRRIAALGVT
                     TLVEIGPGKVLTGLAKRITPDLVLLNVEDPASIDAAVAALDGAAR"
     gene            complement(1672..2664)
                     /locus_tag="VFD06_07430"
     CDS             complement(1672..2664)
                     /locus_tag="VFD06_07430"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZN03406.1"
                     /translation="MSERRAVIAGTGASLPDKVITNADLERMVDTSDEWITSRTGIRE
                     RRVAGENEPMSLFATRAAQAALAAAGTNAADLDLVLCATVTPDMPIPSTACIIQDLLG
                     ARKAAAFDLAAGCSGFLYGLTVAERFLSSPQYESILLIGAEVLSKYVDWKDRTTCVLF
                     GDGAGAVVLKAGAPPSGVLATTLKADGALADFIQVPAGGTREPASAKTIAEGRHYIRM
                     KGNETFKMAVRSMEEAARNVLERAGLETSDVSLFIPHQANLRIIDAVGSRLGLRADQV
                     LINIERVGNTSAASIPVALDEAARAGRLKRGDVVLLAAFGTGLTWGAAVLRWGT"
     gene            complement(2661..>3192)
                     /locus_tag="VFD06_07435"
     CDS             complement(2661..>3192)
                     /locus_tag="VFD06_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HZN03407.1"
                     /translation="SKGNEVTREAFRILKETPLNFIGNVEGRDIFSGKADVIVCDGFI
                     GNVSLKAVESAAEALLHFMKDEISKSALAKIGYLLARPAFRAFRRKVDYAEYGGVPLL
                     GVRGTAIVCHGGSSARAIKNAVRVALEFNRHGVNDRIRDEIVTLTPRERAAEPAPGPA
                     DRAATAADAAATRGGA"
BASE COUNT          422 a         1187 c         1141 g          442 t
ORIGIN      
        1 gtgatgtagg cgccccccgg accgacgagg aacgccaccg cctgggccac gtcctcggcc
       61 gtcccgaggc gctgaagcgg aatggagtcg gccatcttcg tgcgcgccgc ctccggcaga
      121 tccttcgtca tgtccgtgtc gatgaggccc ggggcgacgg cattgacggt gatcgagcgg
      181 gacgccacct cgcgggcgag cgacttggtg agcccgatga ggcccgcctt cgccgcgcag
      241 tagttcgcct gcccgggatt ccccatcagg gccacgatgg aggagatgtt gacgatccgc
      301 ccggagcggg ccttgagcat cgccggcagc acgaggcgcg tcgtctcgaa cgcgccggtg
      361 aggttcgtca ggagcacgcg gtcccattcc tcccgcttca tgcgcaggag gagcccgtcg
      421 ccggtgacgc cggcgttgtt gaccaggtgg tcgatgcggc caccggcccc cttcagggcg
      481 gcgtcgatgc cggccttcac ggaggcggca tcggcgagat cgagggtcac ggcatcggcc
      541 tgtccgcccg cctcgcggca ggcggcggcc gtcgcctcga gccgatcgcg atcgcgcgcc
      601 gcgacgatca cccgcgcgcc gcggcgcgcc agctccagcg cgatcgcccg accgatgccg
      661 cgcgacgcgc cggtgacgag gctgaccttg ccctgcagct cagcggcgct catcgcgccg
      721 ctccgtcgag agccgcgacg gccgcgtcga tcgaggcggg atcctcgacg ttcaagagga
      781 cgaggtcggg ggtgatccgc ttcgcgaggc cggtcaggac cttcccgggt ccgatctcga
      841 cgagcgtggt cacgcccagt gccgcgatgc gtcggaccgt ctcgacccag cggaccggcg
      901 ccgtcacctg ctcctcgagc gccttgcgga atccggccgc cgttcgttcg ggctcggccg
      961 agacgttcgg cagcaccggg aagcgggcgt cctgaagcgg aagcgcggcc agatcccgcg
     1021 tcagccgctc ctgcgccggg cgcatcagcg cgcagtggaa cggggcgctc accgggagct
     1081 cgacgacccg ccgcgccccg agcttcttgg cgacgggcgg cagccggcgc accgcgccgg
     1141 cgtgtccggc gaccacggtc tgatcggggg cgttgaggtt cgcgggggag agcacctccc
     1201 cctccgccgc ctcgcggcac atcgcctcga ccgcgtcgag cggcgcgccg agaaccgccg
     1261 ccatcgcccc ctgcccgacc ggcaccgcct cctgcatgta ctccccgcgc cggcgcacga
     1321 ccctcgccgc gtcctcgagc gcgagcgccc cggccgcgac cagggccgaa tactctccca
     1381 gcgaatgccc ggcggcgcag gcaacgcgcg cgtcgagacc ggggaggcgc gagcgcatcg
     1441 cctcgaggag cgcgacgctc gtcgtcagga tcgccggctg cgtcgtcgcc gtcatccgca
     1501 gcgcctcttc cggcccgtcg aagcaggcgg cgctcagagg gaagccgagg gcccggtcgg
     1561 ccgcgtcgaa gacggcgcgc acctcgggga acgcctcgtg ggccgcccgg cccatgccga
     1621 ccacctgcga tccctggccc gggaagacca gggcgatctt gcgggccccg ctcacgtccc
     1681 ccatcgcagg accgccgccc cccaggtgag cccggtgccg aaggcggcga gcaggaccac
     1741 gtcgccgcgc ttcagccgcc cggcccgggc cgcctcgtcg agcgcgaccg ggatcgaggc
     1801 ggcggaggtg ttccccacgc gctcgatgtt gatcagcacc tgatccgccc ggagcccgag
     1861 gcgcgatccg accgcgtcga tgatgcgcag gttcgcctgg tgcgggatga agagagagac
     1921 gtccgaggtc tcgaggccag cccgctcgag cacgttgcgc gccgcctcct ccatcgacct
     1981 caccgccatc ttgaaggtct cgttcccctt catccggatg tagtgccgcc cctcggcgat
     2041 ggtcttcgcg ctcgccggct cgcgcgtccc cccggccggg acctggatga agtcggccag
     2101 cgccccatcg gccttgagcg tcgtcgccag cacgcccgag gggggcgccc cggccttgag
     2161 caccaccgcc ccggcgccgt cgccgaacag cacgcaggtg gtccggtcct tccagtcgac
     2221 gtacttcgac agcacctcgg cgccgatgag caggatcgat tcgtactgcg gcgaggagag
     2281 gaagcgctcc gccaccgtca gtccgtacag gaacccgctg cacccggccg ccaggtcgaa
     2341 ggccgccgct ttccgcgccc ccagcaggtc ctggatgatg caggcggtcg aggggatcgg
     2401 catgtccggc gtgaccgtgg cgcacaggac gagatcgagg tcggccgcat tcgtcccggc
     2461 cgcggcgagc gccgcctggg cggcgcgggt cgcgaagagg gacatcggct cgttctcccc
     2521 ggcgacgcgg cgctcgcgga tccccgtgcg ggaggtgatc cactcgtcgg aggtgtccac
     2581 catccgctcg aggtcggcgt tggtgatcac cttgtccggg agggacgcgc cggtcccggc
     2641 gatgacggcg cggcgttcgc tcacgcgccg ccccgggtgg ccgcggcgtc cgccgccgtc
     2701 gccgcccgat cggcggggcc gggggccggc tcggcggctc gctcgcgcgg cgtgagggtc
     2761 acgatctcgt cgcggatccg gtcgttgacc ccgtgccggt tgaactcgag ggccacccgc
     2821 accgcgttct tgatggcgcg ggccgacgat ccgccgtggc agacgatggc ggtgccgcgc
     2881 acgccgagga gcggtacgcc gccgtactcg gcgtagtcca ccttgcggcg gaaggcgcgg
     2941 aaggcgggcc gggccaggag gtacccgatc ttggcgagcg ccgacttgct gatctcgtcc
     3001 ttcatgaagt gcaggagcgc ctcggcggcg ctctccaccg ccttgagcga gacgttgccg
     3061 atgaagccgt cgcagacgat gacgtcggcc ttgccgctga agatgtcgcg cccctcgacg
     3121 ttgccgatga agttgagcgg cgtctccttg agaatgcgga acgcctcgcg ggtcacctcg
     3181 ttccccttgc tc
//
LOCUS       DASRIQ010000309         8195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3792256, whole genome shotgun sequence.
ACCESSION   DASRIQ010000309 DASRIQ010000000
VERSION     DASRIQ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792256"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2383
                     /locus_tag="VFD06_07440"
     CDS             <1..2383
                     /locus_tag="VFD06_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN03408.1"
                     /translation="QRTEATGLKVAILGLDGADWEILDPLIKAGRTPSLARLKARGAY
                     GPMKSMDPMLSPILWTTAATGRPPEAHGVIDFLVRDQSTGKPTPVSSRARKVKALWNI
                     LGDVGKTSAFVGWWATWPAEQVEGTIVSDRVAYSTFSFTPDLKDAVGATWPPDYFEKI
                     RPALVAEDKVTDAELRRFADATPDEFRRLAKIVAGARSYHAAGLDLLRAGQPDLFSVY
                     YQGIDEVGHRFQHFMPPRMAMVSDEDFRRYRNAVEEYYVYQDRLVGEMLARLAPDTIV
                     MVLSDHGFRNGTGRPANDPPYVEGKPGLWHRRYGIFIMAGPHVKPGPLDTTSLLDIAP
                     TTLYLLGLPKGEDMPGRVIEEGIDKRFLERFPRHSIPTYESIGHPGPGDSLVAGGAAM
                     GEVEKEMMEKLRSLGYISGGEGGTSEGMGGSPGGAAGGDGPGADTLGGEALITAHINE
                     ATLRLKAKDYARAEASIAEALKLSPEFVPALLLKAQVARERKDYATAIAMGKRVLDMQ
                     PEGERQSYTQMARVFAEAGRIEEGLAYFRAQVTAHPDIGEARAGLGTLLLKAGDNAAA
                     ERELLASLQIDPALGEPLAELHTLYRETAKIPTLEGIVRKGLALNEQSVVHHNWMGLI
                     LEWNKDLPGAEREFRRAMELDPDYAPTMANLGAMYGRSQRLEEAVAILKRAVAKDEGN
                     LEAWVNLGAAQGRLRHPKEAIAALETARGKGMKTTTLYNALALAYLQDGQRTKAVQYL
                     NESLALDPNQQEARELLGAVQRPASKPARRIRRGAARAGGRRSGRAPRGTRSP"
     gene            3047..3562
                     /locus_tag="VFD06_07445"
     CDS             3047..3562
                     /locus_tag="VFD06_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03409.1"
                     /translation="MTKRLAKTGGAYSTNERIARRVYRALKTPADTDRTTDPILGFHF
                     KGRDDYIMLRCNKDPEISIKGKGQTAGEVKFFAYSDFKDCGPQDVLFKLRDEVTLIEL
                     YARGAIEKMTWSSWDDFSEWLVGLREEGVQISLKLPSLVYAAVRHQPPSMQNFIVEAI
                     KLALKRPKGSE"
     gene            complement(3599..4249)
                     /locus_tag="VFD06_07450"
     CDS             complement(3599..4249)
                     /locus_tag="VFD06_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03410.1"
                     /translation="MTSSPSRHALAAVVAAVALAAAAACSQSMKMVRSWSDPALTEGS
                     VKSVFVVGVSESPVIRKSYEDTFVLSLQSKSLKVSPGYELIPDIQQVDKEAVAEQLKK
                     LGVTHVLVTRVVERREVEQYHPPSYVSVGVGYGGYPGYYGGWGSYMSVGYTTMASPGY
                     TTVHTVVSVESNLYDLQTEKMVWTGLSEGYPNDNPTDSFRPYIHQLIYDMKAKKVI"
     gene            complement(4261..4824)
                     /locus_tag="VFD06_07455"
     CDS             complement(4261..4824)
                     /locus_tag="VFD06_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03411.1"
                     /translation="MPKAIYVTVTILFLGLGLGDLTGIHAATPPPPAPPSWKDRMFFG
                     GGIGFGFGDVDFVSVEPLIGIRLDPKLSVGATLLYRYTNDDRFPEEIHFNDYGARAFV
                     QFFPVPNFFGQVEYEYLDYEYVLPNLDTDRTTAGSVFVGGGVFQSIGRGAAFFASTLY
                     NLSYDDNDPTSPYDSPWVVRAGVSVGF"
     gene            5023..6792
                     /locus_tag="VFD06_07460"
     CDS             5023..6792
                     /locus_tag="VFD06_07460"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HZN03412.1"
                     /translation="MTGPQLDPLPFPVSEIRPGLRGVARTVFDGSGLEEFEVEFLGVL
                     RNAIGPQQDMILARLHGEKVEFTGVVAGMSGSPVYIDGRIVGAISYRLGAFAKEPIAG
                     ITPIADMMRAGTSSMRPRIARFETSGSLLGWLERGPERASTPAPGVPGRTDAVPAGSF
                     GNFVPIATPLVCAGCDASVLDYYAPAFQAFGLHPSSGGGGGAAPDTPPDPTPLRPGEA
                     VGAELASGDLSLAAIGTLTHVEGDRVYAFGHPMAGLGPIEVPMTRADVVVTLASAMGS
                     FKLANTTTHVGAIVEDRLTAIVGEVGKTAAVLPMKVRVTSAGGEREFRYDVVRDRTWT
                     PVIAALTTANSMVRSTEFDAAATVAIKSRIVLDGHPEVRYEDLYSGTSPSQPVHIQAA
                     NDVGSLIGLLCNNPFEETVVRSAETLIEILPPSQVASLTSVHAARGEVRAGEPFRVSA
                     VLSPFRGRDRIVSFDVTLPEDTPPGDVQIFVGGGSAMDTLDRRVIERQAAQAKDLDDL
                     LRLVSRQRKSHTLFLRVLRRAPSAIVRSELMPELPLSVFSVFNNPRLSADSSVLLEAP
                     ILEIGRDLDLVATGGRRISVKVK"
     gene            6789..>8195
                     /locus_tag="VFD06_07465"
     CDS             6789..>8195
                     /locus_tag="VFD06_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03413.1"
                     /translation="MNPGNGRSRRAAAGALGALVAFVLLAPPAVAVTSRTWKQRDRAD
                     FDQGEPQGVSLLAEGPIRLGARLESIFEPEQPYIWAVASGPGGVVYAAGGNEGVVYRI
                     EHGKPGAPFFQVEEPAVQALAVDANGAVYAGSGPGGKVYKFNPDGKLVWRTDTLEQYI
                     WSLLFDPKGTLYAATGTQGRVLMIGKDGSSRVIFDSAETHVRTLALDRDGNLLAGTDG
                     HGLILRITPEGKGTVLYDAPLAEVVALTPATDGKLYAAVAGESGKSSRSSSGPRPESS
                     DSGSSSPPSSSQEGSSQTGGAPTEQRLSIGMEGKVLAISPDGYAREVWSGSQEAILSL
                     ARMDNGAVLLGSSGQGRLYLLDSKGTLSEVGRTGASQVTALLPRPAAGGKGTDIVVGG
                     SNLGSVAILKPGYLASGQFESKVFDAQSFATWGRIGWRADVPSGTAVAFQTRSGNTEE
                     PDRTWSDWSPEQSEAAGGR"
BASE COUNT         1304 a         2830 c         2867 g         1194 t
ORIGIN      
        1 gcagcgcacc gaggcgaccg ggctcaaggt ggcgatcctc ggcctcgacg gagccgactg
       61 ggagatcctc gatccgctca tcaaggcggg gcgcacgccg agcctggcga ggctcaaggc
      121 gcgcggcgcg tacgggccga tgaagtcgat ggacccgatg ctgtcgccga tcctctggac
      181 gaccgcggcg accggccggc cgccggaagc acatggcgtc atcgatttcc tcgtgcgcga
      241 ccagtcgacc ggcaagccga cgccggtgtc gagccgggcg cgcaaggtca aggcgctctg
      301 gaacatcctc ggagacgtcg ggaagacctc cgccttcgtc ggctggtggg ccacctggcc
      361 ggccgagcag gtggagggga cgatcgtctc cgaccgggtc gcctacagca ccttttcgtt
      421 cactcccgac ctgaaggacg ccgtcggcgc cacctggccc cccgactact tcgagaagat
      481 tcggcctgcg ctcgtagccg aggacaaggt caccgacgcc gagctgcgcc gcttcgcaga
      541 cgccactccc gacgagttcc gccggctggc gaagatcgtc gccggcgccc gctcgtatca
      601 cgccgccggg ctcgatctct tgagggccgg ccagccggat ctcttctccg tgtattacca
      661 ggggatcgat gaggtcgggc accgcttcca gcacttcatg ccgccgagga tggcgatggt
      721 gtcggatgag gacttccggc gctatcgcaa cgcggtcgag gagtactacg tctaccagga
      781 ccggctcgtc ggcgagatgc tcgcccggct cgccccggac accatcgtga tggtcctgtc
      841 ggaccacggc ttccggaacg gcaccggccg cccggcgaac gatcccccgt acgtcgaggg
      901 gaagccgggg ctgtggcacc ggcgctacgg catcttcatc atggcggggc cgcacgtgaa
      961 gcccgggccg ctcgacacca cgtccctcct cgacatcgcc ccgaccaccc tgtacctgct
     1021 cgggctgccg aagggggagg acatgccggg gcgcgtcatc gaagagggga tcgacaaacg
     1081 cttcctcgag cggttcccgc gccactccat cccgacgtac gaatcgatcg ggcaccccgg
     1141 cccgggcgat tccctggtgg cgggcggggc ggcgatgggg gaggtcgaga aagagatgat
     1201 ggagaagctc cgctcgctcg ggtacatctc gggcggcgag ggagggacct ccgagggaat
     1261 gggcggctcc cccggggggg cggctggcgg agacggaccc ggagcggaca cgctcggcgg
     1321 agaggcgctg atcacggcgc acatcaacga ggccacgctg cgcctcaagg cgaaggacta
     1381 cgcccgtgcc gaggcctcga tcgccgaggc gctcaagctc tcaccggagt tcgttccggc
     1441 gctcctgctc aaggcgcagg tggcccgcga gcggaaggac tacgcgaccg ccatcgccat
     1501 ggggaagcgc gtgctcgaca tgcagccgga aggggagcgc cagtcctaca cgcagatggc
     1561 gcgtgttttc gccgaggcgg ggcgcatcga ggaagggctc gcgtacttcc gcgcgcaggt
     1621 gacggcccac ccggacatcg gcgaggcgcg cgccgggctc gggacgctcc ttctgaaggc
     1681 gggggacaac gccgcggccg agcgggagct cctcgcgtcg ctgcagatcg acccggctct
     1741 cggcgagccg ctcgccgagc tgcacacgct ctatcgcgag accgcgaaga tccccacgct
     1801 cgaggggatc gtccgcaagg ggctggcgct caacgagcag tcggtcgtgc accacaactg
     1861 gatgggactc atcctcgagt ggaacaagga tctgcccggg gcggagcggg agttccggcg
     1921 cgccatggag ctcgatcccg actacgcccc gacgatggcg aacctcgggg cgatgtatgg
     1981 gcgctcccag cgcctcgaag aggcggtggc gatcctcaag cgggcggtgg ccaaggacga
     2041 agggaacctc gaggcgtggg tgaacctcgg cgcggcgcag ggacgcctgc ggcatccgaa
     2101 ggaagcgatc gccgcgctgg agacggcgcg cggcaagggg atgaagacga cgaccctcta
     2161 caacgcgctg gcgctcgcgt acctgcagga tggtcagcgg acgaaggcgg tgcagtacct
     2221 caacgaatcg ctggcgttgg acccgaacca gcaggaggcg cgggagctgc tgggagcagt
     2281 gcagcggccg gcatcgaaac cggcgcgaag gatccggcgg ggcgcagcgc gcgcaggtgg
     2341 tcgccgtagc ggtcgagcgc ctcgcggaac tcgctctcct tgagtccctc gtagcgtccc
     2401 gcccgagcct gcgccacgct ccagccccag gcggccgcct cgaaccagtg gcgctcccgg
     2461 ttcgccccgg accgcgccgg cagcgggatc gtcgtctgcc cggcgaggtg acgcgcgtgg
     2521 aagtactcgt gatcgagcag gagctgcatc cagccgctgc tccgaccgga gcggaacacc
     2581 gccgggtcat agaggatgtc gttgcgcgct ccgcgtcccg ccagcggcgc gctggcgtgt
     2641 gacgtcgcgt cggtggcccc gctccggacc agcaccatgt agacggaggc gcggatcccg
     2701 gccccctccg gcagcggccg gaccgcgctg acgaagttgc gatagtcggg gtgcagcgaa
     2761 gcgaggaggc gctccgccgg atcggtgtcg gcggcgacca taggcgtggt cagggtctcg
     2821 tcggcgacga gcggcgttgt gagggtctcg ccggcgagaa gcggcgtggc caggcaggcg
     2881 gccaggacgc cgggagcggc aagccgcaga agccttgact ggagcggctt tccggcgatc
     2941 ggcatggcgc atcaggtacg ccgaaaggac cgcgtgctca agggagattt gcgccggcgc
     3001 cgctgaaaca tccatggccg ccgtgagccc gtgctatagt cgctttatga caaagcgcct
     3061 agccaagaca ggcggagcgt atagcaccaa cgaacgcatc gctcgccgtg tctaccgtgc
     3121 gctgaagacc cccgccgaca ccgaccgcac gaccgatccg atcctcggct tccacttcaa
     3181 agggcgcgac gactacatca tgctgcgctg caacaaggat ccggagatca gcatcaaggg
     3241 gaaggggcag acggccggcg aggtgaagtt tttcgcctac agcgacttca aggactgcgg
     3301 tccccaggac gtcctgttca agctgcgcga cgaggtgacc ctcatcgagc tttacgcgcg
     3361 cggggcgatc gagaagatga cctggagctc ctgggacgat ttcagcgagt ggctcgtcgg
     3421 cctccgcgaa gagggggtgc agatctcgct gaagctgccg agcctcgtct acgccgccgt
     3481 gcgccaccaa ccgccgtcga tgcagaactt catcgtcgag gcgatcaagc tggcgctcaa
     3541 gcgtccgaag gggtccgagt agccgggccg cgccggccgc gccgccccac gccgcggctc
     3601 agatcacctt cttcgccttc atgtcgtaga tcagctggtg gatgtacggc cggaacgagt
     3661 cggtcgggtt gtcgttggga tacccctcgc tcaggccggt ccagaccatc ttctcggtct
     3721 ggagatcgta gaggttcgac tccacgctga cgacggtatg gaccgtcgtg tagccggggc
     3781 tcgccatggt cgtgtagccg accgacatgt aggagcccca gccgccgtag tacccgggat
     3841 agccgccata gccgacgccc accgagacgt aggagggcgg gtggtactgc tccacctcgc
     3901 gccgctccac cacccgggtc accagcacgt gcgtcacccc gagcttcttc aactgctccg
     3961 ccacggcttc cttgtccacc tgctggatgt ccggaatgag ctcgtagccg ggggagacct
     4021 tgagactctt cgactgcagc gacagaacga aggtgtcctc gtacgacttc cggatcaccg
     4081 ggctctcgga cacgccgacc acgaagacgc tcttcaccga tccctcggtg agggcagggt
     4141 ccgaccagga gcgcaccatc ttcatcgact gggagcaggc cgccgcggcg gcgagcgcga
     4201 cggccgcgac gacggccgcg agagcgtggc gcgacgggga cgaggtcatg ggcactccct
     4261 tcagaaaccg accgagacgc cggcccgcac cacccacggg ctgtcgtagg ggctcgtcgg
     4321 atcgttgtca tcatacgaga ggttgtagag cgtcgaagcg aagaaggcgg ccccccgtcc
     4381 gatcgactgg aacaccccgc ccccgacgaa cacgctgccg gccgtggtgc ggtcggtgtc
     4441 gaggttcggc aggacgtact cgtagtcgag gtactcgtac tccacctgcc cgaagaagtt
     4501 gggcaccggg aagaactgca cgaaggcgcg ggccccgtag tcgttgaaat ggatctcctc
     4561 cgggaagcgg tcgtcgttcg tgtagcggta caggagcgtc gccccgaccg agagtttggg
     4621 gtcgaggcgg atcccgatga gcggctcgac cgagacgaaa tcgacgtctc cgaagccgaa
     4681 accgattcct ccgccgaaga acatccggtc cttccaggaa ggaggcgccg ggggaggcgg
     4741 cgtggcggcg tggattcccg taaggtcacc gaggccgagg ccgaggaaca aaatcgtcac
     4801 cgtgacgtag atcgccttcg gcatgaccaa aacctcctct tttctgcgtc agctccccac
     4861 ttcgggctgg ctttgcccgc agcgtgctgt atcatgcctg aggtgaccga tctacgtagc
     4921 attctctcgt tgtctgctgt cgttctcata cctgccgtcg tcgttgcggt ctgggctgcc
     4981 ccgcccgtcc agacacctcc cgaagtcgcg catccgccgg cagtgaccgg cccccaactc
     5041 gacccgcttc cgttcccagt ctccgagatc cgtcccgggc tccggggggt ggcgcgcacc
     5101 gtcttcgacg ggagcggcct ggaagagttc gaggtggagt tcctcggagt cctgcgcaac
     5161 gccatcggcc cgcagcagga catgatcctg gcccggctgc acggtgagaa ggtggagttc
     5221 accggggtcg tggcggggat gagcggaagc cccgtctaca tcgacggccg catcgtcggc
     5281 gccatctcct accgcctcgg cgcgttcgcg aaggagccga tcgccggcat caccccgatc
     5341 gccgacatga tgcgggccgg cacctcgtcg atgcggccgc gcatcgcgcg gttcgagacc
     5401 tccgggagcc tgctgggctg gctggagcgc gggcccgagc gggcctcgac gccggcgccc
     5461 ggagtgcccg ggaggaccga cgcggtcccg gccgggtcgt tcggcaactt cgtgccgatc
     5521 gccacgccgc tcgtctgcgc cggctgcgac gcctcggtcc tcgactacta tgcccccgcg
     5581 ttccaggcgt tcggcctgca cccctcctcc ggagggggtg gcggggcggc gccggacacg
     5641 ccgcccgatc cgactccgct gcggccgggc gaggcggtcg gcgccgagct cgccagcggc
     5701 gacctgagcc tcgccgccat cggcacgctg acccacgtgg agggcgaccg ggtctacgcc
     5761 ttcgggcacc cgatggccgg cctcgggccg atcgaagtgc cgatgacccg ggcggacgtc
     5821 gtggtcacgc tcgcgtcggc gatgggctcg ttcaagctgg ccaacaccac cactcacgtc
     5881 ggcgccatcg tcgaggatcg cctcaccgcc atcgtcgggg aggtcgggaa gaccgccgcg
     5941 gtcctgccga tgaaggtgcg agtcacctcg gccggcggcg agcgcgagtt ccgctacgac
     6001 gtggtgcggg accgcacctg gacgccggtc atcgccgcgc tgacgaccgc caacagcatg
     6061 gtgcgcagca ccgagttcga cgccgccgcg accgtggcga tcaagtcgcg catcgtgctc
     6121 gacggccatc ccgaggtgcg ctacgaggac ctctactccg gcacgagccc ctcgcagccg
     6181 gtgcacatcc aggcggccaa cgacgtcggc agcctcatcg ggctcctgtg caacaacccg
     6241 ttcgaggaga cggtggtccg ctccgccgag accctgatcg agatcctccc gccgtcgcag
     6301 gtcgcgagcc tgacctccgt gcacgccgcg cgcggcgagg tgcgggcggg cgagccgttc
     6361 cgcgtcagcg ccgtgctcag cccgttccgc ggccgcgatc gcatcgtctc gttcgacgtc
     6421 accctgccgg aggacacgcc tcccggcgac gtgcagatct tcgtgggggg cggctcggcc
     6481 atggacaccc tcgaccgccg cgtcatcgag cgccaggcgg cccaggcgaa ggacctcgac
     6541 gacctgctcc gcctcgtcag ccggcagcgc aagagccaca cgctcttcct gcgcgtcctg
     6601 cggcgggcgc cgtccgccat cgtccgcagc gaattgatgc cggaattgcc gctctcggtc
     6661 ttcagcgtct tcaacaaccc gcggctgagc gccgactcgt ccgtcctgct cgaggcgccg
     6721 atccttgaga tcggccgcga tctcgatctg gtcgcgaccg gcgggcgccg catctccgtg
     6781 aaggtgaaat gaacccaggc aacggccgtt cccgacgcgc cgccgccggc gcgttgggcg
     6841 cgctcgtggc cttcgtcctg ctcgcccctc ccgccgtcgc ggtcacctcc cgcacctgga
     6901 aacagcgcga tcgcgccgac ttcgatcagg gggagcccca gggggtttcg ctcctcgccg
     6961 aagggccgat ccgcctcggc gcccgcctcg agtcgatctt cgagccggag cagccgtaca
     7021 tctgggcggt cgcctccggt cccggcggcg tggtctacgc ggccggcggc aatgaggggg
     7081 tcgtctaccg catcgagcac ggaaagcccg gggcgccgtt cttccaggtc gaggagccgg
     7141 cggtccaggc gctcgccgtg gacgcgaacg gagcggtcta cgccggctcc ggccccggag
     7201 ggaaggtcta caagttcaac ccggacggaa agctcgtctg gcgcaccgac acgctcgagc
     7261 agtacatctg gtctctcctc ttcgatccca aagggaccct ctatgccgcc accgggacgc
     7321 aggggcgtgt tctgatgatc ggcaaggacg gctcgagccg ggtcattttc gacagcgccg
     7381 agacccacgt gcgcaccctc gcgctcgatc gggacgggaa cctcctcgcc gggaccgacg
     7441 ggcacgggct gatcctgcgc atcaccccgg aagggaaggg gacggtgctg tacgacgcgc
     7501 cgctggccga ggtggtggcg ctcacgccgg ccaccgacgg gaagctgtac gccgcggtcg
     7561 ccggggaatc ggggaagtcg tcccgctcgt cgagcgggcc gcgcccggag tcgagcgaca
     7621 gcgggtcgtc ctccccgccg tcctcctcgc aggaggggtc gtcgcagacg gggggcgcgc
     7681 cgaccgagca gcggctgtcg atcggcatgg aggggaaggt gctcgccatc tcccccgatg
     7741 gctacgcgcg cgaagtctgg tccggcagcc aggaagcgat cctgagtctg gcccgcatgg
     7801 acaacggcgc ggtgctgctc gggtcgagcg ggcagggacg gctttacctc ctcgactcga
     7861 aggggacgct gagcgaggtc gggcgcacgg gggcttcgca ggtgaccgcg ctcctgccgc
     7921 gccccgccgc cggcggcaag gggaccgaca tcgtcgtcgg cggcagcaac ctcggcagcg
     7981 tcgcgatcct caagcccggg tatcttgcga gcggccagtt cgagtcgaag gtgttcgacg
     8041 cgcagtcgtt cgcgacctgg ggtcggatcg gctggcgcgc cgacgtcccc tccggcaccg
     8101 cggtggcgtt ccagacccgc agcggcaaca ccgaggagcc ggatcgcacc tggtccgact
     8161 ggagccccga gcagtccgag gcggccggcg gccgg
//
LOCUS       DASRIQ010000310         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2197174, whole genome shotgun sequence.
ACCESSION   DASRIQ010000310 DASRIQ010000000
VERSION     DASRIQ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197174"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFD06_07470"
     CDS             complement(<1..593)
                     /locus_tag="VFD06_07470"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HZN03414.1"
                     /translation="MARSPEGMCESPGTMRRTAGPPRPPARPQDEQADPFEILVEGGP
                     ADDRTLVAMWGDTFGMFDRLGDILPPPRGRNGLFHLGTRYLSRFQLAMWRTRPALLSS
                     SVREEDGMLVVHLTNPDIRSEGAWSMVHHSVHLLRRMWLEDGRCRMELAFRSYGREPL
                     LLPLDILVDADFADIFEVRGALREQRGRLLAPEVDAES"
     gene            complement(598..1698)
                     /locus_tag="VFD06_07475"
     CDS             complement(598..1698)
                     /locus_tag="VFD06_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN03415.1"
                     /translation="MRIAQIAPLYESVPPQGYGGTERVVSYLTEKLVDLGHDVTLYAS
                     ADSDTRARRIGGTPKALRLSGFQGDPLIPHYLMLEQVFAAADRFDVIHSHVDILGLPF
                     ARRGSVPVVTTLHGRLDLEDLVPLYGEYAEQAVVSISDSQREPIPQARWVGTVHHGLP
                     ADLYRFHPEPGRYLAFIGRVSPEKRLDRAIEIAVRARRPLKIAAKVDPVDRKYFRKEI
                     EPLLNHKLIEWIGEVDDRAKDEFLGGAAALLFPIDWPEPFGLTMIESMACGTPVIAWQ
                     HGSVAEVLEHGVTGFLCDDIGAAVRAVGRLDRLSRQVCREVFETRFTADRMARDYLSI
                     YAALIRARRAAGASIRTPGGAFRPETAPIREH"
     gene            complement(1841..>2241)
                     /locus_tag="VFD06_07480"
     CDS             complement(1841..>2241)
                     /locus_tag="VFD06_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03416.1"
                     /translation="LTLDHLGRTDLDAGTATLSGTVTCSHPGTTVVGGTLRQARGTAI
                     TEAEFLVTVECAGTSRFTVTAGEWTPTFRPGRADVSATVGGYEPISRQAASAQIAAVL
                     ALTGAPRPAELPGGTPALTRARPGILRLDR"
BASE COUNT          337 a          785 c          807 g          312 t
ORIGIN      
        1 gactcggcgt caacctccgg cgccagcagc cggccgcgct gctcgcggag ggcgccgcgg
       61 acctcgaaga tgtcggcgaa gtcggcgtcc accaggatgt cgagcggcag gaggagcggc
      121 tcgcggccgt aggagcggaa cgccagctcc atccggcaac ggccgtcctc cagccacatc
      181 cgccgcagca gatggacgct gtgatggacc atcgaccagg ccccctcgct gcggatgtcc
      241 gggttcgtca ggtgcaccac cagcatcccg tcctcctccc ggacgctgct gctcaggagg
      301 gcggggcgcg tgcgccacat ggcgagctgg aagcgggaga gataacgggt ccccaggtgg
      361 aagagaccgt tgcgcccgcg cggcggcggc aggatgtccc ccaggcggtc gaacatgccg
      421 aaggtgtccc cccacatcgc cacgagggtc cggtcgtcgg cggggccccc ttccaccagg
      481 atttcgaatg ggtcggcctg ctcgtcctgc ggccgggcgg gcgggcgcgg gggacccgcg
      541 gtcctgcgca tcgtcccggg actttcgcac atcccttcgg ggcttcgtgc catcgtatca
      601 gtgctcccgg atcggggcgg tctccggccg gaatgcgccg cccggcgtgc ggatcgacgc
      661 gccggccgcg cgcctcgcgc gaatcagagc ggcatagatc gacaggtaat cccgcgccat
      721 ccgatcggcg gtgaagcggg tctcgaacac ctcgcgacag acctgacgag acagccggtc
      781 gagccgcccc accgcgcgca ccgccgcgcc gatgtcgtcg cacaggaacc cggtcacgcc
      841 gtgctccagc acctccgcga ccgacccatg ctgccaggcg atcaccggcg tgccgcaggc
      901 catcgactcg atcatggtca ggccgaacgg ctccggccag tcgatcggga agagcagcgc
      961 cgccgccccg ccgaggaact cgtccttcgc ccggtcgtcc acctctccga tccactcgat
     1021 gagcttgtgg ttcagaagcg gctcgatctc cttgcggaag tacttccggt cgacgggatc
     1081 caccttggcg gcgatcttca gcggccgccg cgcgcggacg gcgatttcga tggcccgatc
     1141 gagccgcttc tccggtgaga cacggccgat gaaggcgagg tagcgccccg gctccgggtg
     1201 gaagcggtag agatcggccg ggagaccgtg gtgcaccgtc ccgacccagc gggcctgcgg
     1261 gatcggctcg cgctgcgagt cggagatcga gacgaccgcc tgctcggcgt actccccgta
     1321 cagcggcacc aggtcctcga ggtcgagccg gccgtggagc gtcgtcacca cgggcaccga
     1381 cccacgacgc gcgaacggca gccccaggat gtccacgtgc gaatggatga cgtcgaagcg
     1441 gtccgccgcc gcgaagacct gctccagcat cagatagtgc ggaatgagcg ggtcgccctg
     1501 gaagccgctc aggcgcagcg ccttcggcgt gccgccgatc cgccgggccc gcgtgtcgga
     1561 atcggcgctg gcgtaaaggg tgacgtcgtg tccgagatcc accagcttct cggtgaggta
     1621 cgagaccacc cgctcggtgc cgccgtaccc ctgcgggggc acgctctcgt agagcggggc
     1681 gatctgggcg atgcgcatgt cggagtcctt cgatatgaac ggcggcggcc ccgtcgcggt
     1741 gaatgcgtcg tgaggttgcc tcgtagtatg aacccgcctc cgcggcacgc caaggtcgcg
     1801 cggcgcgacc ggcggaccgc agcgcggtct cgatggaaga tcagcggtcg aggcggagga
     1861 tgccggggcg agcgcgggtc agggcgggcg tgccgccggg taactcagcg ggcctcggcg
     1921 caccggtcag ggcgaggacg gcggcgatct gcgccgaagc ggcctggcgc gagatcggct
     1981 cgtacccacc gacggtggcg ctgacgtcgg cgcgccccgg acggaaggtc ggggtccact
     2041 cccccgccgt caccgtgaac cgggatgtgc cggcgcattc cacggtgacg aggaactcgg
     2101 cttccgtgat ggcggttccg cgcgcctgtc gcagggtgcc gcccaccacc gtcgtgccgg
     2161 ggtgggagca cgtcacggtc ccggaaagcg ttgccgtccc cgcgtcgaga tcggtgcgtc
     2221 ccaggtgatc gagcgtcagg c
//
LOCUS       DASRIQ010000311         7176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_377073, whole genome shotgun sequence.
ACCESSION   DASRIQ010000311 DASRIQ010000000
VERSION     DASRIQ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377073"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..781)
                     /gene="purN"
                     /locus_tag="VFD06_07485"
     CDS             complement(134..781)
                     /gene="purN"
                     /locus_tag="VFD06_07485"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZN03417.1"
                     /translation="MDPSRRLGVLISGRGSNLLAILDQIRRGALRAEVAVVVCNEPGA
                     AGIGAARRRGVEVVVIDHRASAGREEHDRRMAEVLERHGVALVCLAGYMRLLSPWFIR
                     QFSGRVLNIHPSLLPAFPGTGAQAAALTHGVRVSGCTVHYVNEELDAGPIVLQATVEV
                     RDDDTPAALAARILKDEHRLYSRAIALHLDGRLRIDGRRVSLTGRAPSTRGRRRG"
     gene            complement(841..1029)
                     /locus_tag="VFD06_07490"
     CDS             complement(841..1029)
                     /locus_tag="VFD06_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03418.1"
                     /translation="MDLYAAFLSAMAVLFVVSAVLLLTLAIVQVWRRRGRRAAAYLAL
                     TLLACGVAAGFYLVRGLI"
     gene            complement(1005..2225)
                     /gene="purM"
                     /locus_tag="VFD06_07495"
     CDS             complement(1005..2225)
                     /gene="purM"
                     /locus_tag="VFD06_07495"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZN03419.1"
                     /translation="MRRSRTRTKPVPRRKPAASRPSSGSKPGAPPRPAPARSATAPVK
                     TQRGGLTYKDAGVDIDAKMKAIERIKGVARGTHRRGVLGEIGSFGGLFDLLDAGTWKR
                     PVLVSSTDGVGTKIKVAMRVGDHTTVGQDLVNHCVNDILVQGAIPLFFLDYIAMGKVI
                     PQVLVDLASGMAKGCREAGCTLLGGETAEMPDLYRPGEYDLAGFIVGAVDRSHLIDGA
                     RISSGDVLLGLRSSGLHTNGYSLARKVFFERRGLKPQSLVPELGKSVGEELLAVHRSY
                     LPVLRDLIPGGALYGLAHITGGGFTDNIPRILPKGTAARIDLGSWPVPPIFRLLQRDG
                     RIADDEMLRTFNMGIGMVLVVPAHREAEVMAHLHRMREPYWTIGEIVPGNRKVIYERS
                     ATASREGWTSTPPS"
     gene            complement(2275..4065)
                     /gene="dauA"
                     /locus_tag="VFD06_07500"
     CDS             complement(2275..4065)
                     /gene="dauA"
                     /locus_tag="VFD06_07500"
                     /inference="COORDINATES: protein motif:HMM:NF008660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HZN03420.1"
                     /translation="MHRRLKRAVTTAALRPGRALRDALAAGYGKTDLLADLQAGAIVG
                     IVALPLSMALAIASGVAPQHGLQTAIVAGFVAALLGGSRVQVTGPTAAFVVILAPIAA
                     THGVGGLLVATLLAGLILVAMGTARLGRFIEFVPYPVTAGFTAGIAVVIGTLQVKDFL
                     GLDVARMPEHYLDRIALLVRSLPTAHRGDLLAGIATLTILLAWPKVTRRLPAPLLALT
                     VVTAACWAAGALVPGFEVATIARRFPAAAAGLADGIPRLPPIASWPWSHPGADGLPFH
                     LTWEVIRTLAGPAFAIAMLGAIESLLSATVADGMTGDKHDSDAELVGQGLANIVAPFF
                     GGIAATGAIARTATNVRFGARSPLAAAFHAAFILAAVLLLAPALGHLPMAALAALLLV
                     VAWNMGEFRHVAHVLRVAPRGDVLVLLTCFALTVLFDMVVAVSVGVVLAAFLFMERMA
                     DSARAGLLTEEHPEFLQGLPRNLVVYEVAGPLFFGAAQKAMSTLRTLRKNVKVVVIDL
                     RAVPVIDATGLVNLESAIRRLHEAGIFVVLGGLNRQPHAVLLDAGWHEEPGRLALVDS
                     FAAAVETGRRRLFELEPQAPGAPVQPAPSI"
     gene            4178..5131
                     /locus_tag="VFD06_07505"
     CDS             4178..5131
                     /locus_tag="VFD06_07505"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HZN03421.1"
                     /translation="MLSKLQRDLIRRMQESRSYLRAYEDVEFLKREDLRPVRLQLELL
                     KPELGLREQGINSTIVVFGSTRTVTRRESSRTVRDLERELRKSPRSAALRRRLAIARQ
                     IVANSRFYDEARRFAKIVSSTCQHGGRCDYVIVTGGGPGIMEAANRGAHDVGAKTVGL
                     NITLPHEQYPNAFITPSLSFQFHYFAIRKMHFMMRAKAMVIFPGGYGTLDELFEALTL
                     VQTGKVASLPVILFNRKWWTTLINFGFLRDQGVIAPSDLDLFRFAETAEEAWEKIQEY
                     YRARTGVVIAPGRTPKPGRAAKRQVKVAKRRRTGQVKGTQA"
     gene            5128..6942
                     /locus_tag="VFD06_07510"
     CDS             5128..6942
                     /locus_tag="VFD06_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HZN03422.1"
                     /translation="MTRHQAIPIAGLFLAVAASLSLTTHAFAKEGTAEVPLAEAWKTA
                     YEKSGFLETPRYQETIDYLKRLEKGSPWIRVSSFGKSGEGRDLVLVVASSDRAFDPAA
                     AKKTGKAILLLQAGIHAGEIDGKDAGMMLLRDMAITKTRAALLDGAILLFMPMYNVDG
                     HERFGPYNRTNQNGPREMGWRTTARNLNLNRDYLKADAPETRAWLKVYTTWWPDLLLD
                     AHVTDGADYRPVMTYGVETGPNVPKPVADWAKRAVEGRAVPAIEAGGTLISPYIQLVD
                     DTDPAAGMIAGAATPRFSTGYTVLQNRPAFLLETHMLKGYKSRVDATYATVAALLEEC
                     GRDRKALREAIRSAEEAASRPGRTPLAFRTTEETRPVTLRGVSYTREPSALSGAMRVR
                     YGKEPLDLTVPLRDRVEATLEVERPRAYLVPPQWTDEIAVLKAHGLRLQRLTSPLSTP
                     VEGYRLSDPKWQEAPFEGRHPVTCKAERLESVPRVFPAGTVVVPMDQPGSAVAVHLLE
                     PLGPDSFVSWGFFDAVFEQKEFAEAYVVEELARAMMEKDPALQAEFEKALADPAFAAD
                     PRKRLDFFFRRSPYWENPGLYPIGLVTDPSLKLATRPD"
     gene            complement(6982..>7176)
                     /locus_tag="VFD06_07515"
     CDS             complement(6982..>7176)
                     /locus_tag="VFD06_07515"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HZN03423.1"
                     /translation="IFYTSDEQRKTAEEVKRRVEASGKWSRPIVTEITRASDFWSAEG
                     YHQDYLQKNPGGYTCHFLRD"
BASE COUNT         1110 a         2535 c         2581 g          950 t
ORIGIN      
        1 tcggaggccg ccggttcgac cgggtacgtg gtgtcgtcgg ggggagcggc ggctgcgggc
       61 gcggggttcg gaggcacgcc cgcgggctca gcaagccacg tgcccgggac gggcacacgt
      121 gagcccgctt cgctcagcct ctgcggcgtc cgcgggtgga cggcgcgcgt ccggtgaggc
      181 tcacccggcg cccgtcgatg cgcagccggc cgtcgaggtg gagcgcgatg gcgcgactgt
      241 acagacgatg ctcgtccttc aggatgcgcg ccgccagcgc cgccggcgtg tcgtcgtcgc
      301 gcacctcgac cgtcgcttga aggacgatcg gcccggcgtc gagctcctcg ttcacgtagt
      361 gcaccgtgca gccgctgacg cgcacgccat gcgtcagcgc cgccgcctgg gcgccggtcc
      421 ccggaaacgc gggcagaagc gacgggtgga tgttgagaac ccgtccggaa aattgccgga
      481 tgaaccaggg ggagagaagg cgcatgtacc cggcgaggca gacgagtgcc accccgtgcc
      541 gctccaggac ctccgccatg cgacggtcgt gctcttcgcg gccggcgctc gcgcggtgat
      601 cgatcaccac cacctcgacg ccgcgccggc gcgccgcccc gatcccggcg gcgcccggtt
      661 cgttgcacac cacgaccgcc acctcggctc gcagggcgcc gcggcggatc tggtcgagga
      721 tggcaaggag gttcgagccc cggccggaga taaggacgcc gaggcgacgc gacggatcca
      781 tcggacggcg ggagcggccg gcggggcgcc gcgagcgatg cgcggggcgc cgccgcatcg
      841 tcagatgagg ccgcggacga ggtagaagcc ggcggccacc ccgcaggcca gaagagtcag
      901 cgccaggtag gccgcggccc gccggccacg gcgccgccac acctgtacga tcgcgagggt
      961 gagcagcaga acggcgctca cgacgaacaa caccgccatg gcgctcagga aggcggcgta
     1021 gaggtccatc cctccctcga tgccgtcgcg gagcgctcgt agatcacctt gcggttcccg
     1081 gggacgatct caccgatggt ccagtacggc tcgcgcatcc ggtggaggtg cgccatcacc
     1141 tccgcctcgc ggtgcgccgg tacgaccagg accatgccga tccccatgtt gaacgtccgg
     1201 agcatctcgt cgtcggcgat gcgcccgtcc cgctgcagca gccggaagat cggcggcacc
     1261 ggccagctcc cgagatcgat ccgggccgcg gtccccttcg gcaggatgcg cgggatgttg
     1321 tcggtgaacc cgccgccggt gatgtgcgcg aggccgtaca gcgccccgcc cgggatgaga
     1381 tcccgcagca ccggcaggta ggagcggtgc acggcgagca gctcctcgcc gacgctcttc
     1441 cccagctccg gcacgagcga ctgcggcttg agcccgcgcc gctcgaagaa caccttgcgg
     1501 gcgagggagt agccgttggt gtgcaggccc gacgaccgga ggccgaggag gacgtcgccg
     1561 gaggagatgc gcgccccgtc gatgaggtgg ctccggtcca cggcccccac gatgaagccg
     1621 gcgaggtcgt actcccccgg cctgtagagg tccggcatct ccgccgtctc cccaccgagg
     1681 agggtgcagc cggcctcgcg gcaccccttc gccatcccgg aggccagatc gaccaggact
     1741 tgcgggatca ccttccccat ggcgatgtag tcgaggaaga acagcgggat cgccccctgg
     1801 accaggatgt cgttgacgca gtggttcacc aggtcctgcc cgaccgtcgt gtggtccccc
     1861 acccgcatcg cgaccttgat cttggtgccg acgccgtcgg tgctgctgac caggaccggg
     1921 cgcttccagg tcccggcgtc gaggagatcg aagaggccgc cgaacgagcc gatctccccc
     1981 aggacgccgc ggcggtgcgt accccgggcc acgcccttga tgcgctcgat cgccttcatc
     2041 ttggcgtcga tatcgacccc ggcgtccttg taggtcaggc cgccgcgctg cgtcttgacc
     2101 ggcgcggtcg cggagcgcgc cggggccggg cgcgggggcg cgcccggctt ggatccggac
     2161 gacgggcgcg atgcggcggg cttgcggcgc gggaccggct tcgtccgcgt gcgacttcgg
     2221 cgcacagtgg gcatcggaag ggggcggatt atagcgttcc gccgctcgcg gctgtcaaat
     2281 ggagggcgcc ggctgcaccg gcgccccggg cgcctgtggt tcgagctcga aaagccggcg
     2341 ccggccggtt tcgaccgcgg cggcgaacga atccaccagg gcgaggcgtc ccggctcctc
     2401 gtgccagccg gcgtccagga ggaccgcgtg cggctgccgg ttcagcccgc ccagcacgac
     2461 gaagatcccg gcctcgtgca ggcggcggat cgccgattcg aggttgacga ggccggtcgc
     2521 gtcgatgacc ggcacggcgc gcaggtcgat caccaccacc ttgacgttct tccgcagcgt
     2581 ccgcagggtg ctcatcgcct tctgcgccgc gccgaagaag agcggccccg ccacctcgta
     2641 gacgaccagg ttgcgcggca ggccctgcag gaattccggg tgctcctccg tcagcaggcc
     2701 ggcgcgggcg ctgtcggcca tccgctccat gaacaggaac gccgccagca cgacgccgac
     2761 cgacaccgcc accaccatgt cgaacagcac cgtcagcgcg aaacaggtca gaaggacgag
     2821 cacgtccccc cgcggggcca cccgcagcac gtgggccacg tgccggaact cccccatgtt
     2881 ccaggccacg accaagagca gcgcggccag cgccgccatc ggcaggtggc cgagcgccgg
     2941 cgccagcagg agcaccgcgg cgaggatgaa cgcggcgtgg aacgccgccg ccagcgggct
     3001 ccgcgccccg aaccgcacgt tcgtcgcggt gcgcgcgatg gcgccggtgg cggcgatccc
     3061 gccgaagaac ggcgcgacga tgttcgccag cccctggccg acgagctcgg cgtcggagtc
     3121 gtgcttgtcg ccggtcatgc cgtccgcgac cgtcgccgag aggagcgact cgatcgcccc
     3181 gagcatggcg atggcgaagg ccgggccggc gagggtgcgg atcacctccc acgtgaggtg
     3241 gaacggcagg ccgtccgccc cgggatgcga ccagggccag gaggcgatcg gcggcagccg
     3301 cgggatcccg tccgcgagcc cggcggccgc cgccgggaag cggcgcgcga tggtcgcgac
     3361 ctcgaacccc gggacgagcg ctccggcggc ccagcaggcg gccgtgacga ccgtcagggc
     3421 caggagcggc gccggcaggc ggcgggtcac cttcggccag gccagcagga tggtgagggt
     3481 ggcgatcccg gcgagcagat cgccgcgatg ggcggtcgga agtgaacgga ccaggagggc
     3541 gatccggtcg aggtaatgct ccggcatccg cgccacgtcg agcccgagga agtccttgac
     3601 ctggagggtg ccgatgacga cggcgatgcc ggcggtgaag ccggcggtga ccgggtacgg
     3661 gacgaattcg atgaaccgcc cgaggcgggc ggtccccatc gccaccagga tgagccccgc
     3721 caggagcgtc gccacgagga gtccgccgac cccgtgcgtc gcggcgatcg gcgccaggat
     3781 gacgacgaag gcggcggtcg ggccggtcac ctgcacgcgc gagccgccga gaagcgccgc
     3841 cacgaacccg gcgacgatcg ccgtctgcag gccgtgctgc ggcgcgaccc cggaggcgat
     3901 ggcgagcgcc atcgacagcg gcagcgcgac gatgccgacg atggcgccgg cctgcagatc
     3961 ggccaggagg tcggttttcc cgtacccggc cgccagcgcg tcgcgcagcg cgcgccccgg
     4021 gcgcagggcg gcggtggtga cggcgcgttt caggcgtcgg tgcatcgggg gaccctcatg
     4081 ccggaacggg agattacaat gccgcgcccc ggccccgcat cctcgacggg gctccccgcg
     4141 aagggagccc ggaggcatcg gccggcgagg aggatcgttg ctcagcaagc tgcagcggga
     4201 tctgatccgg cgcatgcagg aatcgcgctc gtatctccgg gcttacgagg acgtggagtt
     4261 cctgaagcgc gaggacctgc ggccggtgcg cctgcagctc gagctcctca agccggagct
     4321 ggggctccgc gaacagggca tcaactcgac gatcgtggtg ttcggcagca cccgcaccgt
     4381 gacgcggcgc gagtcgtcgc ggacggtgcg cgacctcgag cgggagctga ggaaatcgcc
     4441 gcgcagcgcg gcgctgcggc gccgcctggc gatcgcgcgg cagatcgtcg ccaactcccg
     4501 cttctacgac gaggcacggc gcttcgcgaa gatcgtctcg tccacctgcc agcacggcgg
     4561 ccgctgtgac tacgtcatcg tcaccggcgg cggccccggg atcatggagg cggccaaccg
     4621 cggcgcccac gacgtcggcg ccaagacggt cgggctcaac atcaccctgc cccacgagca
     4681 gtacccgaac gcgttcatca ctccgtccct gtcgttccag ttccactact tcgcgatccg
     4741 caagatgcac ttcatgatgc gcgccaaggc gatggtgatc ttccccggcg gctacgggac
     4801 cctcgacgag ctgttcgagg cgctgacgct ggtccagacc ggcaaggtgg cgtcgctgcc
     4861 ggtcatcctg ttcaacagga aatggtggac gaccctcatc aacttcggct tcctgcgcga
     4921 ccagggggtc atcgccccga gcgatctcga tctgttccgc ttcgccgaga cggccgagga
     4981 ggcctgggag aagatccagg agtactaccg cgcccgcacc ggcgtcgtca tcgcgccggg
     5041 gcgcacgccg aagccgggcc gcgccgcgaa gcggcaggtc aaggtggcga agcggcggcg
     5101 caccggtcag gtcaaaggga cgcaggcgtg acacggcacc aggcgatccc gatcgccggg
     5161 cttttcctgg cggtggccgc cagcctctcg ctgaccactc acgcgttcgc gaaggagggg
     5221 acggccgagg ttcctctggc ggaggcgtgg aagacggcgt acgagaagag cgggttcctc
     5281 gagacgccgc ggtatcagga gacgatcgac tacctgaagc ggctcgagaa gggctccccc
     5341 tggatccgcg tctcctcgtt cgggaagagc ggggagggac gggacctcgt tctggtggtc
     5401 gcctcgagcg atcgggcctt cgatcccgcg gcggcgaaaa agaccggcaa ggcgatcctg
     5461 ctcctgcagg ccggcatcca cgcgggggag atcgacggca aggatgccgg gatgatgctc
     5521 ctgcgggaca tggcgatcac gaagacgcgc gccgcgctgc tcgacggggc catcctgctg
     5581 ttcatgccga tgtacaacgt ggacggccac gagcgtttcg gaccttacaa ccgcaccaac
     5641 cagaacggac ctcgagaaat gggctggcgg acgacggcgc gcaacctcaa cctgaatcgc
     5701 gactacctca aagcggacgc gccggagacg cgcgcctggc tcaaggtgta caccacctgg
     5761 tggcccgacc tcctgctcga cgcgcacgtc acggacggcg ccgactaccg cccggtcatg
     5821 acctacggcg tcgagaccgg gccgaacgtc ccgaagccgg tcgccgactg ggcgaaacgc
     5881 gccgtcgagg ggcgggcggt gccggcgatc gaagcgggcg ggaccctgat ctccccctac
     5941 atccagctgg tggacgacac cgatccggcc gccgggatga tcgccggggc cgcgaccccg
     6001 cgcttctcga ccggctacac ggtgctgcag aaccggccgg cgttcctcct cgaaacccac
     6061 atgctcaagg gctacaagtc gcgcgtcgac gccacctatg ccaccgtggc cgcgctcctc
     6121 gaggagtgcg ggcgcgatcg caaggccctg cgcgaggcga tccgctccgc ggaggaggcg
     6181 gccagccgcc cgggcaggac gccgctcgcg ttccgcacga ccgaggagac ccggccggtg
     6241 actctgcgcg gggtgtcgta cacgcgcgag ccgagcgccc tctcgggcgc catgcgggtc
     6301 cggtacggca aggagccgct cgacctgacc gtgcccctca gggatcgtgt cgaggcgacg
     6361 ctcgaggtgg agcggccgcg cgcctatctc gtgccgccgc agtggaccga tgaaatcgcc
     6421 gtcctcaagg cgcacggcct gcgtctgcag cggttgacgt ctccgctgtc cacccccgtc
     6481 gaggggtacc gcctgagcga cccgaaatgg caggaggccc cgttcgaggg gcgccacccg
     6541 gtcacctgca aggcggaacg gctcgagagc gtcccgaggg tgttccccgc cggcaccgtc
     6601 gtcgtgccga tggatcagcc gggctccgcg gtcgccgtgc acctgctcga accgctgggc
     6661 cccgactcgt tcgtgtcgtg ggggttcttc gacgccgtct tcgagcagaa ggagttcgcc
     6721 gaggcgtatg tcgtcgagga gctggcccgc gcgatgatgg agaaggaccc ggcgctccag
     6781 gcggagttcg agaaggcgct cgccgacccc gccttcgccg ccgatccgcg caagcgcctc
     6841 gacttcttct tccggcggag cccgtactgg gagaatcccg ggctctaccc gatcgggctc
     6901 gtcaccgacc cctctctgaa gctcgcgacg cgccccgact gacctcgcgg tcgcgtccgc
     6961 gcgccgcgac cggcgtcgtc gtcagtcgcg caggaagtgg caggtgtagc cgccggggtt
     7021 tttctgcaga tagtcctggt ggtacccctc ggccgaccag aaatccgacg ccctggtgat
     7081 ctccgtgacg atcggccgcg accatttccc cgaggcctcg acgcggcgct tgacctcctc
     7141 cgcggtcttc cgctgctcgt cgctggtgta gaagat
//
LOCUS       DASRIQ010000312         5710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1436145, whole genome shotgun sequence.
ACCESSION   DASRIQ010000312 DASRIQ010000000
VERSION     DASRIQ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436145"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="VFD06_07520"
     CDS             complement(<1..1368)
                     /locus_tag="VFD06_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03424.1"
                     /translation="MMSNRPGPARSWIALAVLALLLLLGGSFPTPAAASGRFDLEALA
                     RMTKVSDPQIAPDGRSIVIIVSRPNYDDNRTDADLVLVDIATGAQRVLTRGRKQLASP
                     RWSPSGDRLAFLAEAPPPPAKKAKGAPAGPQAGPAPAAPAATAAEDPKPARQIHILPM
                     NGGDALVVTDVPEGVQQFAWRPDGGAIAYVTQDPFPDAEARKKGEDAFEVGDNDFMAL
                     EPARPSHVWMIPGEGGEAKRLTSGAWSLPMSEPPGPPSSPIAWSPDGKTLAITRQEKP
                     HFGNADLTTIQLLDVETGALTPITDRKLLEGYPSFSPDGSQIALWYPRDGDPNNVNEI
                     QVVPAAGGTPRSLTRPLDRCLYRALWMPDGKSVLVGGNDGTRVSMWQQPLDGPARKLA
                     LGKVNPSWSFWIDATVGKGGAIAFAGSEPQRPSELYYLASATAEPKRLTAYNDAVASL
                     DLAQVE"
     gene            complement(1457..2887)
                     /locus_tag="VFD06_07525"
     CDS             complement(1457..2887)
                     /locus_tag="VFD06_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HZN03425.1"
                     /translation="MSAAAAPDADRPGPLARAGMAVASACERFFPDAFVVALAAVLLA
                     FGAGLLLGERPATLVRAFGDSFWTLIPFTMQMALVIVGGFTVAGAPPVARLIERLARV
                     PATPRQAVALVALFAMLASLLSWGLSLVFTGLLVRAVIRRVEGIDYRAIGAAAYLGLG
                     SVWALGLSSSAALLQASRASIPAELLPITGVLPLSQTVFLWQSVAMAAILIALSVAVA
                     WWSHPGPGQVRTAASMGVRFESEAEAKGPIDATGRRTMAERLDALPYPGIAVALLLLA
                     WLGTQVRERGVIAALDLNNFNMAFLAAGLLLHGRPASFLRAVVRSVPATAGVLIQFPF
                     YAGIFGLISKTATASALADLFVRVTTRETFPIVVAIYSAVLGLFVPSGGGKWILEAPY
                     VMAAANEWGIHLGWTVQIYNAAEALPNLIHPFWMLPLMGILNVKARDLAGYSVLQLVV
                     HVPVVLLLAWLFTWTLPYVPPNVPAP"
     gene            complement(2884..3981)
                     /locus_tag="VFD06_07530"
     CDS             complement(2884..3981)
                     /locus_tag="VFD06_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HZN03426.1"
                     /translation="MPEPLAPGPGIPDNPPGEPDDNGAPLRLSREPVALPLREPWTIA
                     RGTSAARTNVIVRLWEGEGAPAGLGEAAPNQRYGEDWRSVIAALDRLEPMVAAGRGDW
                     SGLLDRLTLLEPEHPSARAALDILLHDLEARRRGVPLHRLLGADPARMPDTSYSVGLD
                     AVPVMQEKAKQAEAFPFLKIKLDGRDDRAVVEGIRAVTGRPLLVDANEAWTDADEAIE
                     KTRWMRDLGVILVEQPLPAADLDGARRLRARSALPIYADEAVRVVDDLPALAEAYDGV
                     NVKVQKSGGLRPALRIIEAARGLGLEVLIGCMIETSVGITASAHLAPLADRADLDGAL
                     LLASDPFRGASIEAGVITLPDVPGLGAVERR"
     gene            4106..>5710
                     /locus_tag="VFD06_07535"
     CDS             4106..>5710
                     /locus_tag="VFD06_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZN03427.1"
                     /translation="MRRPGVGMLAPAALASLLSGAAHAATVTTYNDEAAFLAALGPAA
                     QRYNADGQASGTPLTTQVAGIAFSSPNAALAGAIPVQVQSSSGAASRPNLVSGGYISG
                     SPGIPQTIVLQFSPYVTAFGAALSSLTPDSVNAMFVATFQDATSQSFPLKTSGSKAVF
                     LGLKADTAITRIEYVAAKGNGGQTGFKNFGIDNLTWLPGDGSVPLCSAEKAVEAGVLG
                     FNGTSTDQAPGDTGIASVTLTAGVNVSLTCSSPFPAACGTAAVPAPSVSWRVAPSAPG
                     LDGSGTVVATDTQGQSCSFDVTFTAFGGGETDDLVVCRDTGIILQVSNGTVATPGQIV
                     CGSTFPGPGEPPYPAGYEPSPDSDPFPCPIFTIKSPISGITAMTLKKDGDFEPRLRLL
                     FSRFDGATFPPFSDITESVEQIDNIIPDPTRVKGSGGWSQVKVACAVQAEVCNGLDDD
                     GDGDVDEGIPEGAQAIDCDHDGYPLCATGASTAIDCSGNTVPLIPGAAADCNDNIAAV
                     NAGAEETCNGLDDDCDGAIDEGAPAGG"
BASE COUNT          841 a         2055 c         2051 g          763 t
ORIGIN      
        1 ctccacctgc gccaggtcga gggaggcgac ggcgtcgttg tacgccgtca ggcgcttcgg
       61 ctcggcggtg gcggacgcca ggtagtacag ctcgctcggg cgctgcggct cgctgccggc
      121 aaaggcgatc gcccccccct tgccgaccgt ggcgtcgatc cagaacgacc aggaggggtt
      181 caccttcccg agagcgagct tgcgcgccgg gccgtcgagt ggctgctgcc acatcgacac
      241 gcgcgtgccg tcgttgcctc cgacgagcac cgacttgccg tccggcatcc agagggcgcg
      301 gtacaggcag cggtcgaggg ggcgcgtcag cgaacgcggc gtgccgcccg cggccggcac
      361 gacctggatc tcgttgacgt tgttcggatc gccgtcgcgt ggataccaca gggcgatctg
      421 cgatccgtcc gggctgaagg aggggtaccc ctccaggagc ttccggtcgg tgatcggcgt
      481 gagcgcgccg gtctcgacgt cgagcagctg aatcgtggtc aggtcggcgt tgccgaagtg
      541 gggcttctcc tggcgcgtga tcgccagggt cttcccgtcg ggggaccagg cgatcggcga
      601 cgacggcggt ccggggggct cgctcatcgg caggctccag gcgcccgagg tgaggcgttt
      661 cgcctccccc ccttcgccgg ggatcatcca gacgtgcgac ggacgggccg gctccagcgc
      721 catgaagtcg ttgtcaccga cttcgaaggc gtcctccccc ttcttgcgcg cctccgcgtc
      781 ggggaacgga tcctgggtca cgtaggcgat ggcgccgccg tcgggacgcc aggcgaactg
      841 ctgcacccct tcggggacgt cggtgacgac gagcgcgtcg ccgccgttca tcggcaggat
      901 gtggatctgt cgcgccggct tgggatcctc cgccgccgtg gccgccgggg cggccggcgc
      961 ggggcccgcc tgcgggccgg ccggcgctcc cttggctttc ttcgccgggg gcggcggagc
     1021 ctcggcgagg aacgccaggc gatccccgga cggcgaccag cgcggggacg ccagctgctt
     1081 gcggccgcgc gtcaggaccc gctgcgctcc ggtggcgatg tccaccagca ccagatccgc
     1141 gtcggtccgg ttgtcgtcgt agttggggcg cgacacgatg atgacgatcg accggccgtc
     1201 cggcgcgatc tgtgggtcgc tgaccttggt catgcgcgcc agcgcctcga ggtcgaagcg
     1261 gccggacgcc gcggcgggcg tcgggaacga ccctcccagg agcagcagca gggccagcac
     1321 ggcgagcgcg atccaggacc gggccggacc ggggcggttg gacatcatcg gtcgtcctcc
     1381 ttcaggagtc gggaagcggg aattatagga cgaacgcggc tccagtccgg cgactcagga
     1441 agcgaccggg ctcggatcag ggcgccggca cgttcggggg gacgtacggc agcgtccagg
     1501 tgaagagcca cgccaggagc agcacgaccg gcacgtgcac cacgagctgc agcaccgagt
     1561 aaccggcgag atcgcgcgct ttcacgttca ggatccccat cagcggcagc atccagaacg
     1621 ggtggatgag gttcggcaac gcctccgcgg cgttgtagat ctgcaccgtc cagccgagat
     1681 ggatgcccca ctcgttggcg gccgccatga cgtagggggc ctcgaggatc cacttcccgc
     1741 cccccgaggg gacgaagagc cccagcaccg ccgagtagat cgccacgacg atcgggaacg
     1801 tctcgcgcgt cgtgacccgg acgaacaggt cggcgagcgc tgatgccgtc gccgtcttcg
     1861 agatcagtcc gaagatgccg gcgtagaacg ggaactggat gagcaccccc gcggtggccg
     1921 ggaccgatcg caccaccgcc cgcaggaacg aggcgggccg cccgtgcagg agcagtccgg
     1981 ccgccaggaa ggccatgttg aagttgttga gatcgagcgc cgcgatgacg ccgcgctcgc
     2041 gcacctgcgt cccgagccac gcgaggagga gaagggccac cgcgatgccg gggtacggga
     2101 gggcatccag gcgctccgcc atcgtgcggc gacccgtcgc atcgatgggc cccttcgcct
     2161 cggcctcgct ttcgaagcgc acccccatcg aggcggcggt ccggacctgg cccggccccg
     2221 ggtgcgacca ccaggcgacc gcgaccgaca gcgcgatgag gatcgccgcc atcgccacgc
     2281 tctgccacag gaagaccgtc tgcgacagcg gcagcacgcc ggtgatcggc agcagctccg
     2341 ccgggatgga ggcgcggctc gcctgcagga gggccgccga cgaggagagt ccgagggccc
     2401 agacgctgcc gagaccgagg tacgcggcgg ccccgatcgc ccggtagtcg atcccctcga
     2461 cgcggcggat caccgcgcgg accaggaggc cggtgaagac gagcgacagg ccccaggaga
     2521 gcagcgaggc gagcatcgcg aacagcgcca ccagcgccac cgcctgccgg ggggtcgccg
     2581 ggacccgcgc cagccgctcg atgagacggg ccacgggcgg cgccccggcc acggtgaatc
     2641 cgccgacgat caccagcgcc atctgcatgg tgaacgggat gagggtccag aaggagtcgc
     2701 cgaaggcgcg caccagggtc gcggggcgct cgccgagcag gagcccggcg ccgaacgcca
     2761 ggagcaccgc ggcgagagcg accacgaacg cgtcgggaaa gaagcgctcg caggcggagg
     2821 cgaccgccat cccggcgcgg gccagcggtc cggggcggtc cgcgtccggg gcggccgccg
     2881 cgctcatcgc cgctccacgg cgcccagccc cggaacgtcg gggagcgtga tgacccccgc
     2941 ctcgatcgag gcgccgcgga acgggtcgct cgcgaggagg agcgcgccgt ccaggtcggc
     3001 ccgatcggcg agcggcgcga ggtgggccga ggcggtgatc ccgaccgacg tctcgatcat
     3061 gcagccgatc aggacctcga ggccgagccc gcgggccgcc tcgatgatcc gcagcgccgg
     3121 gcggagcccc cccgacttct ggaccttgac gttgacgccg tcgtacgcct ccgccagagc
     3181 cggcaggtcg tcgaccaccc ggaccgcctc gtcggcgtag atcggcagcg ccgaccgggc
     3241 gcgcaggcgg cgcgccccgt cgagatcggc ggccggcagc ggctgctcca ccaggatgac
     3301 ccccaggtcg cgcatccagc gggtcttctc gatcgcctca tccgcgtcgg tccaggcttc
     3361 gttcgcgtcc accaggagcg gccggccggt caccgcgcgg atcccctcga cgaccgcccg
     3421 atcgtcgcgg ccgtcgagct tgattttgag gaacggaaac gcctccgcct gcttcgcctt
     3481 ctcctgcatc accggcacgg cgtcgagccc gacggagtac gacgtgtccg gcatccgggc
     3541 cgggtcggcc ccgagcaggc ggtgcagggg gacgccccgg cgccgcgcct cgaggtcgtg
     3601 gaggaggatg tcgagcgcgg cccgggcgga cgggtgctcc ggctcgagga gggtgagccg
     3661 atcgaggagt ccggaccagt cccccctccc cgccgcgacc atgggctcca ggcggtcgag
     3721 ggcggcgatc acgctccgcc agtcctcgcc gtagcgctgg ttgggggcgg cctcccccag
     3781 tccggccggg gctccctccc cctcccacag ccggacgatg acgttggtcc tggcggcgct
     3841 cgtcccccgg gcgatggtcc agggctcccg cagggggagc gcgaccggct cccggctcag
     3901 gcgcaggggt gccccgttgt cgtccggctc acccggcgga ttgtcgggga tgccgggccc
     3961 cggcgcaagc ggctccggca cgcccctttc ccggaggttc cccgagagaa atcctgttga
     4021 atcggggcct tttcatcgca taatttgcgt tcgcccaatt gctttgccgg cattcgtccc
     4081 acacgacact cgcggaggtt tccggatgag acgccctgga gtcgggatgc tcgctcctgc
     4141 ggccctggca tcccttctga gcggagcggc gcacgccgcg acggtgacga cctacaacga
     4201 cgaggccgcg ttcctcgccg ccctcgggcc ggcggcgcag cgctacaacg ccgacgggca
     4261 ggcgtccgga acgccgctca ccacgcaggt cgcggggatc gcgttctcct cgcccaacgc
     4321 ggcgctggcc ggggcgatcc cggtgcaggt gcagtcctcc tcgggggccg cctcccggcc
     4381 gaacctggtc tccggcggct acatctccgg gtcgcccggg attccccaga ccatcgtcct
     4441 gcagttcagc ccgtacgtga cggcgttcgg cgccgccctc tcctcgctga cccccgactc
     4501 ggtcaacgcc atgttcgtgg cgacgtttca ggacgcgacc tcgcagtcgt ttccactcaa
     4561 gaccagcggg agtaaggcgg tcttcctggg gttgaaagcg gacaccgcga tcacccgcat
     4621 cgagtacgtc gcggccaagg gaaacggcgg gcagaccggc ttcaagaatt tcggcatcga
     4681 caacctcacc tggctgccgg gggacggcag cgtgccgctc tgctccgccg agaaggcggt
     4741 cgaggcgggg gtgctcgggt tcaacggcac ctcgaccgat caggccccgg gcgacaccgg
     4801 catcgccagc gtcacgctga ccgccggcgt caacgtgtcc ctcacctgct cgtctccgtt
     4861 cccggccgcc tgcggcaccg ccgccgtgcc ggcaccgtcc gtgtcgtggc gcgtcgcgcc
     4921 gtcggctccg ggcctggacg gatccggaac cgtggtggcg accgacacgc agggccagtc
     4981 ctgctcgttc gacgtcacct tcaccgcctt cggcggcggg gagaccgacg acctggtcgt
     5041 ctgccgcgac acgggaatca tcctgcaggt cagcaacggc accgtcgcga cccccgggca
     5101 gatcgtctgc ggcagcacct tcccgggccc gggcgagccg ccctatccgg ccgggtacga
     5161 gccgtcgccg gacagcgatc cgttcccctg tccgatcttc acgatcaaga gcccgatctc
     5221 cgggatcacc gcgatgacgc tcaagaagga cggcgatttc gagccgcgcc tgcgcctgct
     5281 cttctcgcgc ttcgacggcg cgacgttccc cccgttctcc gacatcaccg agtcggtgga
     5341 gcagatcgac aacatcatcc ccgacccgac gcgcgtgaag gggagcgggg gctggtcgca
     5401 ggtcaaggtc gcctgcgccg tgcaggcgga ggtctgcaac gggctggacg acgacggtga
     5461 cggcgacgtg gacgaaggga tccccgaggg ggcccaggcg atcgactgcg atcacgacgg
     5521 ctacccgctc tgcgccaccg gggcctcgac ggcgatcgac tgctccggca acacggtgcc
     5581 gctcatcccc ggcgcggccg ccgactgcaa cgacaacatc gccgcggtga acgccggggc
     5641 ggaggagacc tgcaacggcc tcgacgacga ctgcgacggc gcgatcgacg aaggggcgcc
     5701 cgccggcggt
//
LOCUS       DASRIQ010000313        12566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_654778, whole genome shotgun sequence.
ACCESSION   DASRIQ010000313 DASRIQ010000000
VERSION     DASRIQ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12566
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654778"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1931)
                     /locus_tag="VFD06_07540"
     CDS             complement(75..1931)
                     /locus_tag="VFD06_07540"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZN03428.1"
                     /translation="MRKLAVVCFLLVVAAVAGGPGLARTQKPAIHARKWVAVTGKPLA
                     ATAGARMFERGGNAVDAACAMLAVTCTMWDTLGWGGETQALVWNPRSKQVIAVNALGV
                     APSGATAEFYRGQGMRYPPEYGPLAAVTPGTPGGLMLMLAEWGKLSLAEVLAPAIEMA
                     GEGYPIERELVRVIGRQKKHIVKWPFSKALFLPLDGQPPEMGSLFQQKDLAATLKRLV
                     EAERAARKAGKDRKAAILAANDLFYRGAIGRELADAAREAGALITAADLARWKPKIET
                     PVSTNYKGIEVYKLTTWTQGPAMLQALNLLEREDLKGMGYNTAPYIHTVTQAMHLAFT
                     DRDFYYGDPDFPPDEPVRGLLSKEYARKRWEEFDPKQNHEKVKPGDPYPFQGGTNPFR
                     DLLDAWPPAQDKKAETQARADADGGMSADEAFRAGTTSVIAADAEGWVVSMTPSGAWV
                     PAVVAGRTGIGLSQRAQAFVLDRRENPFNVIEPGKRPRVTLTPTLALKGGRPYLAFAV
                     QGGDTQDQNLLQFFLNVVEFGFDVQEAAEAANVSTYQMYSSFGKHEIEQGLLEVDLRT
                     PDTVRDALWDMGYRIELAERNSGPINAVLFDHAHGTMRAGSSNQGDDYGIGW"
     gene            complement(2018..2425)
                     /locus_tag="VFD06_07545"
     CDS             complement(2018..2425)
                     /locus_tag="VFD06_07545"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN03429.1"
                     /translation="MSKNGAAEDLARTADGTQRVAPFGAPHVFVLAAVDGADATVVHR
                     LSRPETIVGRGEESHIAIEDDKVSRAHCRIKVEGSVCTLFDSGSRNGTMVNGRKLQSN
                     AGQRLRHLDEIEIGSHRFVLLVGRFREQPKPSA"
     gene            complement(2457..3467)
                     /locus_tag="VFD06_07550"
     CDS             complement(2457..3467)
                     /locus_tag="VFD06_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03430.1"
                     /translation="MRIVRGSIAATVPLLLLACGAPPPPSAPAAPAAPAGPHVTDLMP
                     AFWSFREAAEGKIGADRVALLKDKALAPHREFYEKIVKIPSDERLGQILDTLEPAIPA
                     LQRIDAEFKEQLPRGFDSFVAAYPDMDRRLPIYVGPSLFTSSGQSRDLDGTTIVFYGL
                     DVMAVVLADVTNHLPDIHHELFHAYHWQRNPEIAAASRESFEKTRTAPMYYDLWSEGL
                     ALHAVRRLDPEASLDLVLSSKALPVEGPKALRRVAGELRQRLDVSDLDEVGDYFFFTP
                     KRKDLPSRIAYYVGLRLAEVVGRGRTMEDLVRLGGPELRAAIESGLRTLEEGPGQPPS
                     RP"
     gene            3606..6329
                     /locus_tag="VFD06_07555"
     CDS             3606..6329
                     /locus_tag="VFD06_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN03431.1"
                     /translation="MNRSIGLRVLILLAWLLPGVALAAPAARIAGVLRGPTGEPMAGV
                     EVRLLTPERQPRAVARTGADGGFAFSGVEPGRYIVSVEAPGFEPSEQIVVAGERPISD
                     LTIALKTAGFTETVVVTATPGIPQEAGTTSQAVSTVDEEDLAERAKTVLAQAANEEAG
                     LHVQRTSPTIGAIYVRGFTGAKVNAYVDGIRYSNGAARGGVNTFFNLLDPDYLSGVEV
                     LRGPSSAQYGSDAIGGSVQLLTHAPDLAESGTETSGRFSTHVGSADQSYGASVSGNYA
                     TKTFGLVGTIAGRSVDDTRPGDGIDSHNAVTRYFDLPSDVIIDDELPDTSFDQYGGSL
                     RFTWLPVEGSQFSGALLHGQQEHGQRYDQLLGGDGNLIAELNHVVADLAYVKYDRVLQ
                     GWFDRMSVGVSYNTQREERVNQGGNGNPNASINHEPERTSAYGGQGLVTRFTERQAIS
                     FGADAYFEEAHAPSFSENPTTGATADRRGRIPDGATFRSAGLYAQDTIDAVKGRFQVD
                     GSVRWSGAWYEAQASDAPLNGSGEPLWPDDDLDVSSVTGRLGGVFTIRPGMEILGSVS
                     TGFRAPSITDLGTLGLTGSGFEVAAPDVEGLGGTVGSSADANAVSTGEPVQQLDPEKS
                     VSYEAGFHMRRGRFETEIRGSITNVEDNIAKQTLILPPGAVGQTLGGETITSQNANGA
                     VFVAASPNPVLVRANFDRAQVIGVEFTMNIDLPAHLHLGLVATSLRAEDRDTGLPPNI
                     EGGTPPQEMWLLFRWAPEGRPYWVEPYLHLAAEQDRLSTLDLGDRRTGASRSVSSIAS
                     FFNNGARARGMIDNGGDLTPNTPDDVLIATGETLAQVQSRVLGPSLAASSLYTSVAGY
                     TVYGIRGGFRFAGDSEILLDLENLGDENYRGPSWGMDAPGRGAYIRFATRF"
     gene            complement(6339..7289)
                     /locus_tag="VFD06_07560"
     CDS             complement(6339..7289)
                     /locus_tag="VFD06_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZN03432.1"
                     /translation="MTWVALGLCLSLLAFGVLSAFLTLLLRPLYRRADGSWTSEALLA
                     LRLTPTACAAVAVVGLLLPAFLRFEPRDTGERITWAVLLPAAAGLAMIAAGMLRGVRS
                     LLDARRLRRRLAAGAVAIDLPGAPAPVFVVDHDFPLVALVGVFRPRLFVARRVLAMCT
                     LEEMAAILAHETGHLVRRDNLRQLLVRSCPDVLAWTPSSARLDAAFEEACERDADEDG
                     ARGGRRDDLASALVKVARAVPAPLPATAPYMAFSRSDAVAIRVRRLLFDRPAGGHRPL
                     ARLGAPLLFAATAAAVLVSGWDAALLEVHAVTESVVHLLQ"
     gene            complement(7286..7765)
                     /locus_tag="VFD06_07565"
     CDS             complement(7286..7765)
                     /locus_tag="VFD06_07565"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZN03433.1"
                     /translation="MTVPSLRRLLARRRERSGDRPDPLAAFLGPLEIRVLESLWRRGE
                     ETSVRELVEDFPGSAYTTLMTTLDRLFKKGLLDRGQRERAFLYSARYRRADLERELAK
                     GEIARLLGFAPDRRAARPILSTFVEAVGEKDAVLLDELERLVRARRRRTPSGDGDAR"
     gene            7851..9104
                     /gene="dinB"
                     /locus_tag="VFD06_07570"
     CDS             7851..9104
                     /gene="dinB"
                     /locus_tag="VFD06_07570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZN03434.1"
                     /translation="MPPDDRRVILHLDMDAFYAAIEVRDNPALSGKPIIVGHRGRRGV
                     VSTCSYEARRFGVHSAMPSVTAERLCPQGIWVPVRMERYAEVSHRIFELLRDATPMLE
                     PLSIDEAFLDLTGVAALLEPAGPIMAGLRPVASLEDGRRVAAGLKRRIREAEGLTASV
                     GVAPNKYLAKVASDLEKPDGLLVLSREAIAARLWPLPVTRLWGVGPKTAQHLERAGLT
                     TIGDVARAGETRLAPLVGDRFAGHIAALAHGLDDRPVESDRESKSISEERTYGEDLRD
                     PETIDRALLERSEGVARSLRREGLRARTVHIKVRTADFTTITRAITLDGPTDLTEMIV
                     AAARTLYCDRVQLGGQGVRLLGVGVTGLVHADRGSVPGATGSLFADAGVERARKSAAA
                     ADAVRSRLGDGAITRARLLRSKEEN"
     gene            complement(9130..11958)
                     /locus_tag="VFD06_07575"
     CDS             complement(9130..11958)
                     /locus_tag="VFD06_07575"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03435.1"
                     /translation="MSISSGAARRSAALIALAILVLGARAPAPATAARPLTADDFKGL
                     SFRSIGPSNMGGRASAIALVPGSRTSFYVGFGTGGVFKTENLGTTFTPVFDDQPNLSI
                     GAIAVADAPENWSGWAEEEKKEPPAPPAAPDKAGTKDAEKTRAEKGKGKIVWVGTGEG
                     NGRNSSSWGNGVYRSTDAGRTWTHLGLENTHDIPRLAVDPKNPDVAYVAALGHLWGAN
                     PERGVYKTTDGGKGWKQSLKVDADTGACDVVLDPKGPNTVYAAMYARRRTPWSFTGTS
                     EKGGIFRSSDGGGSWTKLTRGLPPRTGRIGLTVYPKDPRILYAVVESDTGGAAQDAFD
                     NYSKTGGLFRSDDRGDSWTRVSPINFRPFYFSRVAVDPENADRVYLPGWDLAISDDGG
                     KTFRGSGSPKVHVDFHAIVVNPLDPNQILVGNDGGVYVSHDRAATWDYLNRLAVGQFY
                     RIAVDDSDPYRVAGGLQDNGSWMGPSETFFESEDAGPGGEAREDGILSEDWRTIFFAD
                     GFTVQFDPVDRNVVYATGQGGAVARVRLDNNVKTSIAPAVREGQERIRFNWNAPYFVS
                     PHDPTVLYMGGNKVFRLTDRGNHFEAISPDLSKNEPVKTATVGSDAETYGTVVSLAES
                     PLVAGMLWAGTDDGRVHVTKDGGKSWSDVTPKEVGGLYVSRLAPSRHQREACALAVDG
                     HRSDDFRTLLFLTEDAGRTWKSITGDLPPKEPVEVVTEALLNKETLYAGTEFGLYVTV
                     DRGAHWARLNGRSLPPAPVDDLVIHPRERDLVVATHGRSLYVLDDITPIAQLTPENRA
                     KPLVLFKPMPATPRLYTGRNYGGGAGMFRASNPSMGATVTYWLRDGNPDGVSLSIADS
                     AGTVLREINGPGRPGLNRVVWDLQADRKHRIPTIDTNRLGQTQFVPAGDYTITAKLDT
                     GTPGRPEKSETTVTVKAAPTDLPKPSGR"
     gene            complement(12002..>12566)
                     /locus_tag="VFD06_07580"
     CDS             complement(12002..>12566)
                     /locus_tag="VFD06_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03436.1"
                     /translation="AYLINPENDNGVATYRGNAADLLDDTPGSEAYMSVADQYLYRVG
                     ATWFPSPKVGLSLGLRWEGIPVHDLVGDSEGFRRPGYAFSYEPGLSYTTGPNTFALNI
                     PIAAHRNRERSVSDLEDGGAGDAAFADYVIIFGWARRLGKQAQQHIDAPVPPPPPPPS
                     EPSSSPGASRSSVAPAGCQTPETRTSR"
BASE COUNT         1826 a         4444 c         4439 g         1857 t
ORIGIN      
        1 gctgtgcagt tcatcctcga cggcctgcgc gccgccgagg tgttccgcct ctgatcactt
       61 tcgcttgaac tccttcacca gccgatgccg tagtcgtccc cctggttgga ggacccggcg
      121 cgcatcgtcc cgtgcgcgtg gtcgaacagc acggcgttga tcgggccgga attccgctcc
      181 gcgagctcga tcctgtagcc catgtcccac aacgcgtcac gcaccgtatc gggcgtgcgc
      241 agatcgacct ccagcagccc ctgttcgatc tcgtgcttcc cgaacgacga gtacatctgg
      301 tacgtactga cgttggccgc ctccgccgcc tcctgcacgt cgaagccgaa ttcgacgacg
      361 ttcaggaaga actgcaggag gttctggtcc tgcgtgtctc ccccctgcac cgcgaacgcc
      421 aggtaggggc gccctccctt gagggcgagc gtcggcgtga gcgtgacgcg cgggcgcttt
      481 cccggctcga tgacgttgaa cggattctcg cgccggtcga ggacgaacgc ctgggcccgc
      541 tgcgacagcc cgatgccggt gcgccccgcc acgaccgccg gcacccaggc gccggacggc
      601 gtcatcgaca ccacccagcc ctccgcgtcg gcggcgatga ccgaggtcgt gccggcgcgg
      661 aacgcctcgt ccgccgacat gccgccgtcg gcgtcggcgc gcgcctgcgt ctccgccttc
      721 ttgtcctgtg ccggcggcca ggcgtcgagg aggtcgcgga acgggttcgt cccaccctgg
      781 aacggatagg ggtcccccgg tttcaccttc tcgtggttct gcttcgggtc gaactcctcc
      841 cagcgcttgc gggcatactc cttcgacagg agcccgcgga ccggctcgtc cggcggaaag
      901 tcgggatcgc cgtagtagaa gtcgcggtct gtgaaggcga ggtgcatcgc ctgcgtcacc
      961 gtgtggatgt agggggcggt gttgtacccc atccccttga gatcctcgcg ttcgagcagg
     1021 ttcagcgcct gcagcatcgc cggcccctgc gtccaggtgg tcagcttgta gacctcgatc
     1081 cccttgtagt tcgtcgacac cggcgtctcg atcttcggct tccagcgcgc caggtcggcg
     1141 gcggtgatga gcgccccggc ctcgcgcgcc gcgtcggcca gctcgcgccc gatggcgccg
     1201 cggtagaaga ggtcgttcgc cgcgagaatc gccgccttgc gatccttgcc cgccttgcgg
     1261 gccgcccgct cggcctcgac cagccgcttc agggtggcgg cgaggtcctt ctgctggaaa
     1321 agcgacccca tctccggcgg ctggccgtcg agcggcagga agagggcctt ggagaacggc
     1381 cacttcacga tgtgcttctt ctgccggccg atgacccgga ccagctcgcg ctcgatcggg
     1441 tagccttcgc cggccatctc gatcgccggc gccagcacct ccgcgaggga cagctttccc
     1501 cactcggcga gcatcagcat cagcccgccg ggggtccccg gcgtcacggc ggcgagcgga
     1561 ccgtactcgg gcgggtagcg catcccctgg ccacgataga actccgcggt cgccccgctc
     1621 ggcgcgaccc cgagcgcgtt caccgcgatc acctgcttcg agcgcgggtt ccagacgagc
     1681 gcctgggtct cgccgcccca cccgagcgtg tcccacatgg tgcaggtgac ggcgagcatg
     1741 gcgcaggcgg cgtcgaccgc gttgccgccg cgctcgaaca tccgcgcccc cgccgtcgcg
     1801 gcgagcggct tgccggtgac cgccacccac ttgcgcgcgt ggatcgccgg tttctgcgtc
     1861 cgcgccagac ccggcccacc cgcaacggcc gcaaccacca gcaggaaaca gacgaccgcc
     1921 agcttgcgca tctcacctcc cggggtgaat cctggattct aacgccgcgg agggaaaggc
     1981 gggggatcgg cgggcgctga tcgatgctat cgagctttta ggcggagggt ttgggctgct
     2041 cgcggaagcg acccaccagg agaacgaagc ggtggctgcc gatctcgatc tcgtcgaggt
     2101 ggcgcagccg ctgcccggcg ttggactgga gcttccggcc gttgaccatg gtgccgttgc
     2161 ggctgccgct gtcgaacagg gtgcacaccg agccttccac cttgatgcgg cagtgggccc
     2221 ggctcacctt gtcgtcttcg atggcgatgt ggctctcctc accgcgcccg acgatggtct
     2281 cgggtcggct gagccggtgc acgacggtcg cgtccgcccc gtccacggcc gccagcacga
     2341 agacgtgcgg ggcgccgaac ggggccaccc tctgggtccc gtcggcggtg cgggccagat
     2401 cctcggcagc gccgtttttc gacatgcctt gaatgttggg accggaaggc tcgctgtcaa
     2461 ggccgggagg ggggttggcc gggtccctcc tcgagcgtcc ggagacccga ttcgatcgcc
     2521 gcccgcagct cgggaccgcc gaggcggacg aggtcctcca tggtgcgccc gcgcccaacg
     2581 acctccgcca gccgcagacc gacgtagtag gcgatgcggg acggcaggtc cttccgcttc
     2641 ggcgtgaaga aaaagtaatc ccccacctcg tcgagatcgg agacgtcgag gcgctgacgc
     2701 agctccccgg cgacgcggcg cagcgccttc ggcccttcga cagggagcgc cttggaggag
     2761 agcacgaggt cgagcgacgc ttccgggtcc agccgtcgca ccgcgtggag ggcgagcccc
     2821 tcggaccaga ggtcgtagta catcggcgcc gtgcgggtct tctcgaacga ttcccggctc
     2881 gcggcggcga tctcggggtt ccgctgccag tgataggcgt ggaacaactc gtggtggatg
     2941 tccgggaggt ggttcgtcac gtcggcgagg accaccgcca tcacgtcgag cccgtagaag
     3001 acgatcgtcg ttccgtcgag atcgcgcgac tgcccggacg aggtgaacag ggagggcccg
     3061 acgtagatgg gaagcctccg gtccatgtcg ggataggccg cgacgaagga gtcgaatccc
     3121 cggggcagct gttccttgaa ctcggcgtcg atccgttgca gcgccgggat cgcgggctcc
     3181 agcgtgtcga gaatctggcc gaggcgctcg tccgagggaa tcttcacgat cttctcgtag
     3241 aactcgcggt gcggcgccaa cgccttgtcc ttcaggagcg ccacacggtc cgccccaatc
     3301 ttcccctccg ccgcctcgcg gaacgaccag aacgccggca tcaggtcggt cacgtgcggc
     3361 ccggccggcg ccgcgggggc cgccggtgcg gatggggggg gcggcgcgcc gcaggcgagc
     3421 aggagcagcg ggacggtggc ggcgatcgag ccacggacga tgcgcaaggc gttccctcct
     3481 ccgcgctatt ccacgcgggc cccgccccgg cggttcccgg gatcccccgg cctctacgat
     3541 ggctggtagt agtccaccgg cggccttcag gattccatca cgttcgaatc ggggggatcc
     3601 gcatgatgaa ccgcagcatc ggccttcgcg tcctgatcct gctcgcctgg cttctgcccg
     3661 gagtcgccct ggcggccccg gcggcgagga tcgccggagt cctgcgcggc cccacgggcg
     3721 agccgatggc cggggtggag gtgaggctcc tcacccccga acgacagccg cgcgccgtcg
     3781 ccaggaccgg cgccgacggc ggcttcgcct tcagcggcgt cgagcctggc cgctacatcg
     3841 tgtcggtcga ggccccgggg ttcgagccca gcgagcagat cgtcgtggcc ggagagcggc
     3901 cgatctcgga cctgacgatc gcgctcaaga ccgccgggtt caccgagacg gtcgtcgtca
     3961 ccgccactcc cggcatccct caggaagccg gcacgacatc gcaggcggtc agcaccgtgg
     4021 acgaggagga tctggcggag cgcgcgaaga cggtcctggc gcaggcggcg aacgaggagg
     4081 cggggctgca cgtccagcgc accagtccca ccatcggtgc gatctacgtg cgcggcttca
     4141 ccggcgccaa ggtcaacgcc tacgtggacg gtatccgcta ctccaacggc gcggcgcgcg
     4201 gcggggtgaa taccttcttc aacctgctcg accccgacta cctgtcgggc gtcgaggtcc
     4261 tgcgcggccc ctcgagcgcg cagtacggca gcgacgcgat cggcggcagc gtgcagctcc
     4321 tgacgcacgc tcctgacctc gcggagagcg ggaccgagac ctccggccgc ttcagcacgc
     4381 acgtcggcag cgccgaccag agctacgggg cgtcggtgtc ggggaactac gccacgaaga
     4441 cgttcgggct cgtcggaacc atcgccggcc ggagtgtgga cgacacgcgc cccggagacg
     4501 ggatcgactc gcacaacgcc gtcacacgct atttcgacct cccctccgac gtcatcatcg
     4561 acgacgagct gcccgacacc tccttcgatc aatacggcgg ctcgctccgc ttcacctggc
     4621 tgccggtcga agggtcgcag ttcagcggcg cgctcctgca cggccagcag gagcacgggc
     4681 agcgctacga tcagctcctc ggcggcgacg gcaacctcat cgccgagttg aaccacgtcg
     4741 tcgccgacct cgcctacgtc aagtacgacc gggtgctcca gggttggttc gatcggatga
     4801 gcgtcggcgt gtcgtacaac acgcagcgcg aggagcgggt gaaccagggg gggaacggca
     4861 acccgaacgc gtcgatcaac cacgagccgg agcgcacctc cgcctacggc gggcagggtc
     4921 tggtcacccg cttcacggag cggcaggcga tctcgttcgg cgccgacgcc tacttcgagg
     4981 aggcgcacgc gccgtcgttc tcggagaacc cgacgaccgg cgcgaccgcc gatcgccgcg
     5041 gccgcatccc cgacggcgcc accttccgga gcgccggtct ctacgcccag gacaccatcg
     5101 acgcggtgaa ggggcgcttc caggtggacg gcagcgtgcg ctggagcggc gcctggtacg
     5161 aggcgcaggc ctccgacgcc ccgctcaacg gctcggggga gccgctgtgg ccggacgacg
     5221 atctcgacgt gtcgagcgtc accggccgcc tcggcggcgt gttcacgatc cggccgggga
     5281 tggagatcct cgggagcgtc agcaccggct tccgcgcccc gagcatcacc gacctcggca
     5341 ccctggggct gaccggatcg gggttcgagg tcgccgcccc cgacgtcgag gggctggggg
     5401 gaaccgtcgg ctcgagcgcc gacgccaacg ccgtctccac cggcgagccg gtgcagcagc
     5461 tcgatccgga gaagagcgtg tcgtatgagg ccggcttcca catgcggcgc ggccgcttcg
     5521 aaaccgagat ccgcgggtcg atcacgaacg tcgaggacaa catcgccaag caaacgctga
     5581 tcctgccgcc gggggccgtc ggccagacgc tcggcggcga gacgatcacc agccagaacg
     5641 ccaacggtgc ggtgttcgtg gccgcctctc ccaacccggt gctggtgcgc gccaacttcg
     5701 accgcgccca ggtcatcggc gtcgagttca ccatgaacat cgacctgccg gcccacctgc
     5761 acctcgggct ggtcgcgacc tcgctgcgcg ccgaggaccg ggacaccggc ctgccgccga
     5821 acatcgaggg ggggactccc cctcaggaaa tgtggctgct cttccgctgg gcgccggagg
     5881 gccgaccgta ctgggtcgag ccgtatctgc acctggcggc ggagcaggat cggctgtcga
     5941 cgctcgactt gggagatcgc cgcaccggcg ccagccggtc ggtgagttcg atcgcctcct
     6001 tcttcaacaa cggcgcgcgc gcccgtggga tgatcgacaa cggcggcgac ctgacgccga
     6061 acacgccgga cgacgtcctg atcgccaccg gtgagacgct ggcgcaggtg cagtcgcgcg
     6121 tgctcggacc gagcctcgcc gcaagctccc tctacacctc ggtcgcgggg tacaccgtgt
     6181 acgggatccg gggcgggttc cgcttcgccg gggacagcga gatcctcctc gatctcgaga
     6241 acctcgggga tgagaactac cgcggcccgt cctgggggat ggacgccccg ggccgcggcg
     6301 cctacatccg cttcgcgacc cgattctagg agcctggact actgcagcag atgtacgacc
     6361 gactcggtca cggcgtgcac ctcgagcagc gcggcatccc agcctgacac cagaaccgcc
     6421 gcagcggtcg ccgcgaaaag cagcggcgcg ccgaggcgcg cgaggggacg gtggccaccg
     6481 gccggccggt cgaagaggag ccggcggacg cggatcgcga ccgcgtcgct ccgggagaac
     6541 gccatgtacg gggccgtggc ggggagcggc gccggcacgg cgcgcgcgac cttcaccagc
     6601 gccgaagcga gatcgtcgcg gcgtccgccg cgcgccccgt cttcgtccgc gtcccgctca
     6661 caggcttcct cgaaggccgc atcgagacgc gcgctggacg gagtccatgc caggacgtcg
     6721 ggacaggagc gcaccaggag ctgcctgagg ttgtcgcggc gcacgaggtg cccggtctcg
     6781 tgggcgagga tggcggccat ctcctccagc gtgcacatcg cgagcacccg ccgcgcgacg
     6841 aacagccgcg gccggaacac cccgaccagc gccacgagcg ggaagtcgtg atcgacgacg
     6901 aacaccggtg ccggcgcccc ggggagatcg atcgccaccg ccccggcggc gagtcgccgc
     6961 cgcagccggc gcgcgtcgag caacgaccgc acgccgcgca gcatcccggc ggcgatcatc
     7021 gccaggcccg ccgctgcggg gaggagcacc gcccaggtga tgcgctcgcc ggtgtcgcgc
     7081 ggctcgaatc gcaggaaggc cgggaggagc agaccgacga ccgcgaccgc ggcgcaggcc
     7141 gtcggcgtca ggcgcagggc gaggagcgcc tcgctcgtcc aggatccgtc ggcgcgccgg
     7201 tagagcgggc gcaggaggag cgtcagaaaa gcgctgagca ccccgaacgc caggagggac
     7261 aggcagagcc cgagcgccac ccaggtcatc gcgcgtcacc gtccccggag ggcgtccgcc
     7321 gccgccgggc gcgcaccagc cgctccagct cgtcgagcag gaccgcgtcc ttctccccga
     7381 ccgcctccac gaaggtggac aggatcggac gcgccgcccg ccggtccggg gcgaatccaa
     7441 ggagtcgcgc aatctccccc ttcgccagct cccgctcgag atcggcgcgc cggtatcggg
     7501 cgctgtagag aaaggcgcgc tcgcgctgtc cgcgatcgag gagccccttc ttgaagaggc
     7561 gatcgagggt cgtcatcaga gtggtgtagg ccgaaccggg gaagtcctcc accagctccc
     7621 ggacgctcgt ctcctcgccg cggcgccaca gcgactcgag gaccctgatt tcgagggggc
     7681 ccaggaaggc cgcgagaggg tcggggcgat cgccggaacg ttcgcgccgg cgcgccagca
     7741 accgcctgag ggagggaacc gtcacgaaac ctccggaaac gccccaatcg tacccgaatt
     7801 ccgcggccgg cgggacgatt cgcggggtat gctcatcttc gagcccttcc atgccccccg
     7861 acgaccgccg cgtcatcctg cacctcgaca tggacgcgtt ctacgcggcg atcgaggtgc
     7921 gcgacaaccc cgcgctatcg ggcaagccga tcatcgtcgg gcaccgcggg cggcggggag
     7981 tggtcagcac ctgctcctac gaggcgcggc gcttcggcgt ccactcggcg atgccgtcgg
     8041 tcaccgcgga gcgcctgtgc ccccagggga tctgggtgcc ggtgcggatg gagcggtacg
     8101 cggaggtgtc gcaccggatc ttcgagctcc tccgcgacgc gactccgatg ctcgagccgc
     8161 tgtcgatcga cgaggcgttc ctcgacctga ccggcgtcgc agcgctgctc gagccggccg
     8221 gccccatcat ggcggggctg cggccggtcg ccagcctcga ggacggccgg cgggtcgccg
     8281 ccgggctcaa gcggcggatc cgggaggccg aggggctgac cgcctcggtc ggcgtcgccc
     8341 cgaacaagta cctggcgaag gtcgcgtccg acctggagaa accggacggc ctgctcgtcc
     8401 tgtcgcgcga ggcgatcgcg gcgcggctct ggccgctgcc ggtgacccgg ctgtgggggg
     8461 tggggccgaa gaccgcccag cacctcgagc gcgccgggct cactacgatc ggcgacgtgg
     8521 cgcgcgccgg cgagacccgt ctggcgccgc tcgtcggcga tcggtttgcc ggccacatcg
     8581 ccgccctggc gcacggcctc gacgaccgcc cggtcgagag cgaccgggag agcaagtcga
     8641 tctccgagga gcggacttac ggcgaagacc tgcgcgaccc cgagacgatc gaccgggcgc
     8701 tgctggagcg atcggagggg gtggcccgct cgctgcggcg cgaggggctg cgggcgcgca
     8761 ccgtgcacat caaagtgcgc acggcggact tcacgacgat cacgcgggcg atcacgctcg
     8821 acggaccgac cgacctcacc gagatgatcg tcgccgccgc ccgcacgctc tactgcgatc
     8881 gggtgcagct cggcgggcag ggggtgcggc tcctcggcgt cggcgtgacc ggcctcgtcc
     8941 acgccgaccg gggatcggtt cccggcgcga ccggctcgct gttcgcggac gcgggggtgg
     9001 agcgggcgag gaagtcggcc gcggcggccg acgcggtccg ctcacgcctc ggggacggtg
     9061 ccatcacacg ggcgcgtctg ctgcgatcga aagaagagaa ctagggagcc aggaggcctc
     9121 gcgcgcgatt catcgaccgg acggcttcgg gagatcggtc ggcgccgcct tcaccgtcac
     9181 ggtcgtctcc gacttctccg ggcgtcccgg cgtcccggtg tcgagcttcg cggtgatcgt
     9241 gtagtcgccg gccggcacga actgcgtctg ccccaaccgg ttggtgtcga tcgtcgggat
     9301 ccggtgcttg cgatcggcct gcaggtccca caccacccgg ttgagacccg gccggccggg
     9361 accgttgatc tcgcggagca ccgtaccggc cgagtcggcg atcgacaggc tgacgccgtc
     9421 cgggttgccg tcccggagcc agtaggtcac ggtcgccccc atcgacggat tgctggcgcg
     9481 gaacatcccc gctccgcctc cgtagttgcg gccggtgtac aggcgcggcg tcgccggcat
     9541 cggcttgaac aggacgagcg gcttcgcccg gttctccggc gtcagctgag cgatcggcgt
     9601 gatgtcgtcg aggacgtaga gcgatcggcc gtgcgtcgcc acgaccaggt cccgttcccg
     9661 cgggtggatc accaggtcgt ccaccggcgc cggcggcagc gaccggccgt tcagccgcgc
     9721 ccagtgggcg ccgcggtcga ccgtcacgta gagcccgaac tcggtgccgg cgtagagggt
     9781 ctccttgttc aggagcgcct cggtcacgac ctcgaccggc tccttcggcg ggagatcgcc
     9841 ggtgatgctc ttccaggtgc gtccggcgtc ctcggtcagg aagagcagcg tgcggaagtc
     9901 gtcgctgcgg tgcccgtcca ccgccagggc gcacgcctct ctctggtgcc gtgacggcgc
     9961 caggcgcgac acgtacagtc cgccgacttc cttcggcgtc acgtcgctcc agctcttccc
    10021 gccgtccttc gtcacgtgca cccggccgtc gtcggtgccg gcccagagca tcccggcgac
    10081 gaggggagat tcggcgagcg acacgaccgt gccgtaggtc tcggcgtcgg atcccacggt
    10141 cgcggtcttg accggctcgt tcttcgagag gtcggggctg atcgcctcga agtggttccc
    10201 ccgatcggtc aggcgaaaga ccttgttccc ccccatgtag aggaccgtcg gatcgtgcgg
    10261 cgagacgaag tacggcgcgt tccagttgaa gcggatccgc tcctgcccct cccgcaccgc
    10321 cggggcgatc gaggtcttga cgttgttgtc gaggcggacg cgggcgacgg cgcccccctg
    10381 accggtggcg tacacgacgt tgcgatcgac cgggtcgaac tgcaccgtga agccgtcggc
    10441 gaagaagatc gtccgccagt cctccgacag gatcccgtcc tcacgcgcct ccccgccggg
    10501 gccggcgtcc tccgactcga agaaggtctc cgacggcccc atccaggagc cgttgtcctg
    10561 cagcccgccg gccacccggt acgggtcgga gtcgtccacg gcgatgcggt agaactggcc
    10621 gaccgccagc cgattcaggt agtcccaggt ggcggcgcga tcgtgcgaga cgtagacgcc
    10681 gccgtcgttg ccgaccagga tctggttcgg gtcgagcggg ttgacgacga tggcgtggaa
    10741 gtcgacgtgc accttggggc tcccggagcc gcggaaggtc ttcccgccgt cgtcggagat
    10801 cgccaggtcc cagcccggga ggtagacgcg gtcggcgttc tccggatcga ccgcgacgcg
    10861 cgagaagtag aacggccgga agttgatcgg gctgacgcgg gtccaggagt cgccgcggtc
    10921 gtcgcttcgg aagagcccgc cggtcttcga gtagttgtcg aacgcgtcct gcgcggcgcc
    10981 ccccgtgtcg ctctcgacga cggcgtagag gatgcgcggg tctttcgggt acacggtcag
    11041 gccgatgcgc ccggtgcggg gcgggagtcc gcgggtcagc ttcgtccacg acccgccacc
    11101 gtccgacgag cggaagatcc cccccttctc gctggtgccg gtgaacgacc agggggtgcg
    11161 gcggcgcgcg tacatcgcgg cgtagacggt gttcggcccc ttcggatcga gcacgacgtc
    11221 gcacgcgccg gtgtcggcgt ccaccttgag cgactgtttc caccccttgc cgccgtcggt
    11281 cgtcttgtag acgccgcgct cgggattcgc gccccacagg tggcccagcg ccgcgacgta
    11341 cgcgacgtcg gggttcttgg gatcgacggc gaggcgcggg atgtcgtggg tgttctcgag
    11401 gcccaggtgc gtccaggtgc gcccggcgtc ggtcgatcga tagacgccgt tcccccacga
    11461 cgacgagttg cggccgttcc cctcgccggt gccgacccag acgatcttcc ccttcccctt
    11521 ctcggcgcgc gtcttctccg catccttcgt ccccgccttg tccggggcgg cgggcggcgc
    11581 gggcggctct ttcttctcct cctcggccca gccggaccag ttctcggggg cgtccgccac
    11641 cgcgatggcg ccgatcgaga ggttcggctg atcgtcgaac accggcgtga acgtcgtgcc
    11701 gaggttctcg gtcttgaaca cgccgccggt gccgaagccc acgtagaacg aagtgcggct
    11761 gccggggacg agcgcgatcg ccgacgccct gcctcccatg ttggagggac cgatgctccg
    11821 gaacgacagc cccttgaagt cgtcggcggt gagcggacgc gccgccgtcg ccggcgcggg
    11881 ggcgcgggcc ccgaggacga gaatggcgag cgcgatcagc gcggccgatc gccgtgcggc
    11941 tcctgacgaa atcgacatgg ccccccccgc agtcgtgggt tgtgtggatc gggggcggat
    12001 tctaccgcga ggtgcgcgtc tcgggggtct ggcagccggc cggcgccacg gaagaccggc
    12061 tggcaccggg ggacgatgac ggctcggagg ggggcggcgg cgggggcggc accggcgcgt
    12121 cgatgtgctg ctgcgcctgc ttcccgagcc tccgggccca tccgaagatg atcacgtagt
    12181 cggcgaaggc cgcgtccccc gcgccgccgt cctcgagatc cgagacgctc cgctcgcggt
    12241 tgcgatgggc tgcgatcgga atgttgaggg cgaaggtgtt cggcccggtg gtgtacgaga
    12301 gcccgggttc gtacgagaag gcgtacccgg gccgccggaa tccttcgctg tcgccgacga
    12361 gatcgtggac cgggatcccc tcccagcgca gccccaggct caatcccacc ttggggctgg
    12421 ggaaccaggt ggcgccgacc ctgtagagat actggtccgc gaccgacatg tatgcttcgc
    12481 tccccggcgt gtcgtccagg agatcggcgg cgttgccgcg ataggtcgcg acgccgttgt
    12541 cgttctccgg gttgatgaga tacgcg
//
LOCUS       DASRIQ010000314         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1692205, whole genome shotgun sequence.
ACCESSION   DASRIQ010000314 DASRIQ010000000
VERSION     DASRIQ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692205"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1292)
                     /locus_tag="VFD06_07585"
     CDS             complement(126..1292)
                     /locus_tag="VFD06_07585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HZN03437.1"
                     /translation="MKAVCWHGKHDVRVDTVRDPRIVNPHDAIVKVTATGICGSDLHL
                     YNALVPAMIRGDILGHEFVGVVVETGPEVRNLRKGERVVVPFAIACGRCFFCRREMNS
                     LCENTNPNAVMAEKMWGYSPAGLFGYSHLTGGYAGGQAEYVRVPFADVGPVKIPDGLT
                     DEQVLFLSDILPTGYAAVENCGLTPGDTVAVWGCGPVGQFAIRCAYLLGAERVIAIDR
                     IPARLRMAEEGGAETLDYEEVDLAEALDDRTGGRGPDACIDAVGMEAHAPGVQGAYDR
                     AKQAVRLETDRPAVLRQTLTMCRNGGMVSVPGVYGGFIDKVPFGSAMNRSLTIRSGQT
                     HVHRYIRLLLERIVKGDIDPTFVITHRLPLDKAQKGYEMFHQKDDDCIKVVLKP"
     gene            complement(1289..1753)
                     /locus_tag="VFD06_07590"
     CDS             complement(1289..1753)
                     /locus_tag="VFD06_07590"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN03438.1"
                     /translation="MKSITVDGTPEECYRAWRDMEGYPRFLSFVEAVRPHREDRFHWV
                     VREGAGDPIEWDVDLVEDRPGEALVWRSIPGSDVEMVGGVWFERSTGGRGTVVRLGLD
                     YAPRTGALKAFVAGLVEKVQLKKELHRFKQWFETGEVATTEGQPSGREGDDA"
     gene            1897..>2490
                     /locus_tag="VFD06_07595"
     CDS             1897..>2490
                     /locus_tag="VFD06_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN03439.1"
                     /translation="MIIRLAAASLILLSASCHDVREEYDLVIRGGTIVDGTGAPRVAG
                     DVAIRGDRIAAVGAIPGRGRKEIDARGLIVAPGFIDMHSHSDYLLLEDGAAQSKIRQG
                     VTTEVFGEESSGGPMKGNYRGRAKLMGDTEATRVWATLGAYFETLEKSRMSVNVASFV
                     GQGNIWRCVMGDSYERPTPAQIGEMKALVAEAMKDGAF"
BASE COUNT          384 a          880 c          826 g          400 t
ORIGIN      
        1 gaggcagcgc ctggtcggga tagcccttct cgcggagcac gtcgaagacg gcgccactgt
       61 agcactgttc caggcggtcg gagaaactca cgggccagat tgtagtcggg atggcccgcg
      121 atcgcctacg gtttcagcac caccttgatg cagtcgtcgt ccttctggtg gaacatctcg
      181 tatcccttct gcgccttgtc gagcggcagc cggtgggtga tgacgaaggt cgggtcgatg
      241 tcgcccttca cgatccgctc cagcagcagc cggatgtagc ggtggacgtg cgtctgcccg
      301 gaccggatcg tcagagaccg gttcatggcg gagccgaacg gcaccttgtc gatgaacccg
      361 ccgtagacgc cgggaaccga aaccatcccg ccgttccggc acatcgtcag cgtctgccgc
      421 aggaccgcgg ggcggtcggt ctcgagcctc acggcctgct tcgcccggtc gtaggcgccc
      481 tggacgcccg gcgcgtgggc ctccatcccg accgcgtcga tgcacgcgtc ggggccccgg
      541 ccgcccgtcc gatcgtccag cgcctcggcc aggtcgacct cttcgtagtc gagcgtctcc
      601 gctcctccct cctctgccat gcggagccgg gccgggatgc ggtcgatcgc gatgacccgc
      661 tcggccccca ggagatacgc gcatcggatc gcgaactgcc ccacggggcc gcagccccag
      721 acggcgaccg tgtcgcccgg ggtcagaccg cagttctcga cggccgcgta cccggtgggg
      781 aggatgtcgg agaggaacag gacctgctcg tccgtcagcc cgtccgggat cttgaccggg
      841 ccgacgtcgg cgaacgggac gcgcacgtat tcggcctgtc cgcccgcgta cccgcccgtc
      901 aggtgggagt agccgaacag gcccgccgga gagtagcccc acatcttctc cgccatgacg
      961 gcattggggt tggtgttctc gcagagcgag ttcatctccc tgcggcagaa gaagcagcgg
     1021 ccgcaggcga tggcgaacgg gaccaccacc cgctccccct tccggagatt gcggacctcg
     1081 gggcccgtct cgacgaccac tcccacgaat tcgtggccga ggatgtcgcc ccggatcatc
     1141 gccggcacca gcgcgttgta caggtggaga tccgagccgc agatccccgt cgccgtgact
     1201 ttgacgatcg cgtcgtgggg attgacgatc cgcgggtcgc gcaccgtgtc gacgcggacg
     1261 tcgtgctttc cgtgccagca caccgccttc aagcgtcatc cccttcccga cccgagggct
     1321 ggccctcggt cgtcgcgacc tcgcccgtct cgaaccactg cttgaagcgg tggagctcct
     1381 tcttcagctg gaccttctcg accaggccgg ccacgaacgc cttcagggcg cccgtccgcg
     1441 gcgcgtagtc caggcccagc cggacgaccg tgccccggcc ccccgtcgac cgctcgaacc
     1501 agaccccgcc gaccatctcg acgtctgacc ccgggatcga ccgccagacc agcgcttcgc
     1561 ctgggcgatc ctcgaccaga tcgacatccc actcgatggg gtcgccggcc ccctcccgca
     1621 cgacccagtg aaaccggtct tcccgatggg gccggacggc ctcgacgaag ctcaggaagc
     1681 gggggtatcc ctccatgtcg cgccacgcgc gatagcactc ctcgggcgtg ccgtcgaccg
     1741 tgatgctctt catccggagg ccgggctccg tccccttccg ggatggagcg ccgcgtcggc
     1801 gagcgatttg agtcgccatg gactcggcct ccgttaaaat gatgccctat tcggtagagc
     1861 cgtcgggtcc tcagggggtt attcggtaaa cgtttcatga ttatcagact tgccgccgcc
     1921 tcgctcatcc tgctctcggc atcctgccac gacgtccgcg aggagtacga tctcgtgatc
     1981 cgcggcggga cgatcgtcga cggcacggga gccccccgcg tcgccggcga cgtcgccatc
     2041 cgcggcgacc gcatcgcggc cgtcggcgcg attcccggcc ggggccggaa ggaaatcgac
     2101 gcgcggggcc tcatcgtggc ccccggattc atcgacatgc actcccattc ggactacctg
     2161 ctgctcgagg acggcgccgc gcagagcaag atccggcagg gggtgacgac cgaggtcttc
     2221 ggcgaggaga gctccggcgg gccgatgaag ggcaactacc gcggccgcgc gaagctgatg
     2281 ggcgacaccg aggcgacccg ggtgtgggcg acgctgggcg cgtacttcga gacgctcgag
     2341 aagtcgagga tgtcggtgaa cgtcgcctcc ttcgtcggac aggggaacat ctggcgctgc
     2401 gtgatgggcg actcgtacga gcggcccacc ccggcgcaga tcggggagat gaaggcgctc
     2461 gtcgcggagg ccatgaagga cggcgcgttc
//
LOCUS       DASRIQ010000315         5950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1949284, whole genome shotgun sequence.
ACCESSION   DASRIQ010000315 DASRIQ010000000
VERSION     DASRIQ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949284"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="VFD06_07600"
     CDS             complement(<1..1977)
                     /locus_tag="VFD06_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZN03440.1"
                     /translation="MRDTRGRRGSLLRVVTVFLSAAACSSGASSRDAAAPPSAAAGAA
                     AGAIDTEALFAANNRGAALMEQYKPAQAIEEFARVTALAPGWAPGHANLGLAALYARQ
                     MDRAMQEFKEAIRIDPALVAGHYGLATLEKGQGRSAEALTGFEAAQKLDPKDADILYN
                     IGLLYGRQRQFDRAIEALRQAKEIDPNNMSVRYQLARAYLQSGKAAEGEKEMAVYQKL
                     AANPKFAVPTGNQYGEAGRYALVTTDYAGLSPAATPAPAVAVRFHAAADSGLVFRHAG
                     PGGEAKPGDAARLGSGVAVGDLDGDGLPDLVFANAAEGKAAPPAVFRNRGKGTFEDVT
                     AASGVTFASAGLGAALGDYDNDGDLDLCLTGERGTGLLRNEGKGTFKDVTTTARASVA
                     GLAAGAAWGDLDHDGDLDLLLTRPAPAQGKTRVVLLRNRGDGAFDDATSTLPVKPGAA
                     GAIGAIFSDLDLDRDLDIVVAGIGGPDVFLDNRREEGFADAGSKAGLRAQGGGRGVAV
                     GDVNDDGRPDLVFPAAASEKTALFLGAAEGGFTRRDLPPLSGGSAYGAVLFDADNDGD
                     LDLYLAGSGQALLLNDGRGGFADAGQGSGLAAVPVKDGRGAAAADFDGDGDLDLVVST
                     NGGAPLLLMNDTPAQNRWVEIAPRGLNSNRQAVGT"
     gene            complement(1995..3650)
                     /locus_tag="VFD06_07605"
     CDS             complement(1995..3650)
                     /locus_tag="VFD06_07605"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN03441.1"
                     /translation="MLDLILADAEVIDGSGGPRRRMDVGLEGDRIAAVAPRLAAESAR
                     RLDLRGLVLAPGFIDLHSHHDLVLALPPSRQKDLLEGRLRQGITTEVVGNCGIGVSPV
                     EATHAEEVRKVCGFIAPDGVAWEWRTLAEWLDRIERQGVLVNVATLAAHGPARAAVMG
                     SRAGRPEPQEQQRMERLLREAVEAGAFGISFGLIYPPGQFADTDEVAGCAAAAAAAGG
                     FAAFHQRGSSLPTLLPAVQEIVEAGRRSRAPNHHSHVETVGPRAWPGTEEVLRLHAEA
                     ERNGIPVSGDVIPYTAVCTTLLALLPPWSLDGGVLALLERLRDPATRRRMGAEIAERD
                     AAWPPWEDPSRFTMNIAKECGWGRIRLAHVDGAPNKRFEGRSLLAIARERDVAPFDAL
                     ADLLLEERGVATQLIFGISGDDRGDEHLVPFLRAPHLAFVSDAWEIGKGAPHPGAYGA
                     FPRVLGHYVRERRLLGLEEAVRRMTSLPAGRLGLRDRGVVREGARADLVAFDPARVAA
                     LSDYDAPRRLPAGIEHVFVNGRAQVEAGQYRPTAAGHVLRRAA"
     gene            complement(3657..3992)
                     /locus_tag="VFD06_07610"
     CDS             complement(3657..3992)
                     /locus_tag="VFD06_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03442.1"
                     /translation="MSAIDPDKEAGARGMRVVEPPSDAPEPEAGPGEARRSIEILRCT
                     YRPPGADFYCWKYGVWYYLMECCLRHDENTFEGCGDCGQGRNNLKANLARYLHDRKRR
                     TQFGPKSLA"
     gene            4080..5144
                     /locus_tag="VFD06_07615"
     CDS             4080..5144
                     /locus_tag="VFD06_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZN03443.1"
                     /translation="MRPLLRALACTAPLALAACGGGPPIPPEEVVKVPAEGTVVVFVE
                     APRYIAGPILKMFSEQTGIVVQATYREEAGPGFADQLDAAVAAGKADLLWGASPLLAI
                     ELQSKDRLIPFRPAGARAVPLQYHDRSYRWIGFAANPRVILAHDSDAARDRPPAVLED
                     LVVAPWAGKAVLARPAEGTAAFQAAALFARLGSSRGREFFERVAAAGNLVVEDDAEVR
                     RLVAAGERDWGFVDLDRAICSKRQGEPVSIIFPDRLGMGAVAIPEVIALLKGAPNPDQ
                     ARGLIGYLFSTDAIWAIGQNDCALISLLPVNALGIPKPEWVPVLSALNVQAIDNQKVF
                     DAWTQNRSFLKSWGTAATAR"
     gene            5244..>5950
                     /locus_tag="VFD06_07620"
     CDS             5244..>5950
                     /locus_tag="VFD06_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN03444.1"
                     /translation="MRRATHARPALSMSDRESTAPGASQRPAAGPPTRRFRVLVVDDD
                     ADSVDLLHRWLAMEGYDVTTAKDGAEALQRIAAERPDLVLLDLLIPAPEGLQVIRTVK
                     RDRAMSTVPIVVMTVKRDVKTKIEALRTGADDFIVKPFHFDELDAVMRASLKKRYLYT
                     SLERANRALRDANEKLTKLSVTDDRTNLLNDRYLKRRLSEEFKRARRYGAPLTVMILD
                     LDHFKQVNDKYGHDCGD"
BASE COUNT          836 a         2219 c         2060 g          835 t
ORIGIN      
        1 cgtcccgacc gcctgtcgat tggaattgag gccgcggggg gcgatctcca cccagcggtt
       61 ctgcgcgggg gtgtcgttca tcaggaggag cggggcgcca ccgttggtcg aaacgaccag
      121 atcgaggtcg ccgtcgccgt cgaaatcggc ggcggcggcg ccgcggccgt ccttcaccgg
      181 caccgccgcg agcccggacc cctgccccgc gtcggcgaag ccgccgcggc cgtcgttgag
      241 caggagcgcc tggccactgc cggcgagata aaggtccagg tcgccgtcgt tgtcggcgtc
      301 gaacaggacc gcgccgtacg ccgatccgcc cgaaaggggt ggaagatcgc gccgcgtgaa
      361 ccctccctcg gctgctccca ggaagagggc cgttttctcg gaggccgcgg ccgggaacac
      421 gaggtcggga cgaccgtcgt cgttgacgtc cccgacggcc acgccgcgcc cgcccccctg
      481 ggcccgcagc cccgccttgc tcccggcatc ggcgaaccct tcctcgcgcc ggttgtcgag
      541 aaagacgtcc ggtccgccga tcccggcgac cacgatgtcg agatcccggt cgagatcgag
      601 gtcggagaag atcgcgccga tcgcccctgc cgcacccggc ttcaccggca gggtcgaggt
      661 ggcgtcgtcg aaggcgccgt cgccgcggtt gcgcagcagg acgacgcgcg tcttcccctg
      721 agccggggcg ggccgcgtga gcaggagatc gagatcgccg tcgtgatcga gatcccccca
      781 cgccgccccc gccgcgagcc ccgcgaccga ggcccgggcc gtcgtcgtga cgtctttgaa
      841 cgtgcccttg ccctcgttgc gcagcagccc tgtgccgcgc tcgcccgtga ggcacagatc
      901 gaggtcgccg tcgttgtcgt agtctccgag cgccgctccc agccccgcgg aggcgaaggt
      961 cacccccgaa gcggccgtga cgtcctcgaa cgtccccttc ccccggttcc ggaagaccgc
     1021 cggcggggcc gcctttcctt cggcggcgtt ggcgaaaacc agatcgggaa ggccgtcgcc
     1081 gtccagatcg ccgaccgcca ctccggatcc cagccgcgca gcgtcccccg gcttcgcttc
     1141 gccgccgggc ccggcgtggc ggaacacgag accggaatcg gccgcggcgt ggaaccgcac
     1201 cgccaccgcc ggcgccggcg tcgcagcggg agagagacca gcgtagtcgg tggtgacgag
     1261 ggcgtagcgc cccgcctcgc cgtactggtt gccggtcggg acggcgaatt tcgggttggc
     1321 ggccagcttc tggtagaccg ccatctcctt ctccccttcg gccgccttgc ccgactggag
     1381 ataggcgcgc gccagctggt agcgcaccga catgttgttc ggatcgatct ccttcgcctg
     1441 gcgcagcgcc tcgatggcgc gatcgaactg gcgctggcgg ccgtagagaa gcccgatgtt
     1501 gtagaggatg tcggcgtcct tcgggtcgag cttctgcgcc gcctcgaacc cggtcagcgc
     1561 ctcggcgctc cgtccctgcc ccttctcgag cgtcgcgaga ccatagtgac cggcgacgag
     1621 cgccggatcg atccggatgg cttccttgaa ctcctgcatc gcgcgatcca tctggcgcgc
     1681 gtacagcgcc gcgaggccga ggttggcgtg cccgggcgcc cagccggggg cgagagcggt
     1741 gactctggcg aattcctcga tcgcctgcgc cggcttgtac tgctccatca gcgccgcgcc
     1801 gcggttgttg gcggcgaaga gcgcctcggt gtcgatcgca cccgccgccg cacccgccgc
     1861 tgcggaaggc ggggccgccg catcgcgcga cgaggccccg ctcgagcagg cggccgccga
     1921 caggaagacc gtgacgaccc gcagcagcga cccgcgccgg ccgcgtgtat cacgcatgca
     1981 agagcctcct cctctcatgc ggcccgcctc agcacatgcc ccgcggcggt gggccggtac
     2041 tggccggctt cgacctgcgc ccgcccattg acgaagacgt gctcgattcc ggccggcaga
     2101 cgccgcggcg catcgtagtc ggagagcgcc gcgacacgcg ccggatcgaa cgcgacgaga
     2161 tcggcccgcg ccccctcgcg caccacaccg cgatcgcgca ggccgaggcg gccggccggc
     2221 agcgacgtca tgcggcgcac cgcctcctcg agaccgagca gccgccgttc gcgcacgtag
     2281 tgcccgagca cccgcgggaa cgctccgtac gcgccggggt gcggcgcccc cttgccgatc
     2341 tcccaggcat cgctgacgaa cgccaggtgc ggcgcccgga ggaacggcac caggtgctcg
     2401 tcgccgcgat cgtcgccgga gatcccgaag atgagctgcg tcgcgacccc acgctcctcg
     2461 aggaggagat cggcgagcgc gtcgaagggc gccacgtcac gctcgcgggc gatcgccaga
     2521 agcgaccgcc cctcgaacct cttgttcggg gccccgtcca cgtgcgcgag gcggatccgc
     2581 ccccagccgc actccttggc gatgttcatg gtgaagcgcg agggatcctc ccaggggggc
     2641 caggcggcgt cccgctcggc gatctccgcc cccatccgcc ggcgggtcgc cggatcccgc
     2701 agcctctcga gcagtgccag gaccccgccg tcaagcgacc agggcggaag cagggccaaa
     2761 agcgtcgtgc agaccgccgt gtacgggatg acatcgccgc tcacggggat gccattccgc
     2821 tccgcctcgg cgtgcagtcg caggacctcc tccgtccccg gccaggcgcg cggtccgacc
     2881 gtctcgacgt gcgaatggtg gttcggcgcg cggctgcggc ggccggcctc cacaatctcc
     2941 tgcaccgccg gcaggagcgt gggcaggctg ctgccgcgct ggtggaacgc cgcgaacccg
     3001 cccgccgcgg cggccgcggc ggcgcacccc gcgacctcat cggtgtcggc gaactggccg
     3061 ggcgggtaga tgaggccgaa cgagattccg aacgcccccg cttcgaccgc ctcgcgcagc
     3121 aggcgctcca tgcgctgctg ctcctgcggc tccggccggc ccgcgcggct ccccatgacg
     3181 gcggcccgcg ccggtccgtg cgcggcgagg gtcgcgacgt tcacgagcac cccctgccgc
     3241 tcgatccgat cgagccactc cgcgagcgtc cgccactccc aggcgacccc gtcgggcgcg
     3301 atgaatccgc agaccttccg gacctcctcg gcgtgcgtcg cctccacggg agagacgccg
     3361 atgccgcagt tgccgaccac ctcggtcgtg atcccctggc gcagccgccc ttcgagcagg
     3421 tctttctggc gggacggcgg cagcgccagc acgagatcat ggtgcgagtg caggtcgatg
     3481 aagccggggg cgaggacgag gcctcgcagg tcgagccgcc gcgccgactc ggcggcaagc
     3541 cggggggcga cggcggcgat gcgatcccct tcgaggccga cgtccatgcg ccggcggggc
     3601 ccaccgctcc cgtcgatcac ctcggcgtcg gcgaggatca gatcgagcac gtaatctcag
     3661 gccagggatt tcggtccgaa ctgggtgcgg cgcttccgat cgtggaggta gcgggccagg
     3721 ttcgccttga gattgttgcg cccctggccg cagtcaccgc acccctcgaa ggtgttctcg
     3781 tcgtgccgca ggcagcactc catgaggtag taccagacgc cgtacttcca gcaatagaaa
     3841 tcggcgcccg gcgggcggta ggtgcagcgg aggatctcga tcgaccgccg ggcctccccg
     3901 gggccggcct caggctccgg cgcgtcggag gggggctcga ccacccgcat cccgcgcgcc
     3961 cccgcttcct tgtccggatc gatggcgctc atggcccggg tattctagcc cggatcgcgc
     4021 ggggcatcgg gccggcctcg gttgcggccc gacggtgatg cggctaccgt atccgcccca
     4081 tgcgtcccct gcttcgggcc ctcgcgtgca cggcaccgct cgcgctcgcc gcctgcggcg
     4141 gcgggccgcc gattcccccc gaggaagtcg tcaaggtccc cgcggagggg acggtcgtcg
     4201 tcttcgtcga ggccccgcgc tacatcgccg ggccgatcct caagatgttc agcgagcaga
     4261 ccggcatcgt cgttcaggcg acgtaccgcg aggaggccgg ccccggcttc gccgaccagc
     4321 tcgacgccgc ggtcgccgcc ggcaaggccg atctcctctg gggggcgagc ccgctgctgg
     4381 caatcgagct gcagtcgaag gatcggctga tcccgttccg tccggccggg gcgcgcgcgg
     4441 tgccgctcca gtaccacgac cgctcctacc gctggatcgg cttcgccgcc aacccgcggg
     4501 tgatcctggc gcacgacagc gacgctgccc gcgaccgccc gcccgcggtg ctcgaggacc
     4561 tcgtggtggc gccgtgggcc ggcaaggcgg tgctcgcccg ccccgccgag ggaaccgcgg
     4621 cattccaggc ggccgccctc ttcgcccggc tcgggtcctc gcgcggccgc gagttcttcg
     4681 agcgggtggc tgcggccggc aacctggtcg tcgaggacga cgccgaggtg cgccggctgg
     4741 tggccgccgg cgagcgggac tgggggttcg tcgatctcga ccgggcgatc tgctccaagc
     4801 gtcaggggga gccggtgtcg atcatcttcc ccgatcggct cgggatgggg gcggtggcga
     4861 ttccggaagt gatcgcgctt ctcaaggggg cgccgaaccc cgatcaggcg cgcggcctca
     4921 tcggctacct gttctcgacc gacgccatct gggccatcgg ccagaacgac tgcgccctca
     4981 tcagcctcct gccggtgaac gccctcggga tcccgaagcc ggagtgggtg ccggtgctca
     5041 gcgccctgaa cgtccaggca atcgacaacc agaaggtgtt cgacgcctgg acacagaacc
     5101 gctccttcct gaaatcctgg gggaccgccg cgaccgcccg ctgacgtcgc cgtcggcggc
     5161 gtgacgccgg ggcaaatgcc gggaaaccgt tgcgggccgg ggcttttccg ttgcccgcct
     5221 ctccgggcgg tggtatatca ttcatgcgtc gcgcgacaca cgcgcggccg gccctctcca
     5281 tgagcgacag agaatcgaca gccccgggcg cctcccagcg cccagccgcc ggtcccccca
     5341 cgcgccgctt ccgcgtcctc gtggtggacg acgacgccga cagcgtcgac ctgctccacc
     5401 gctggctggc gatggagggg tacgacgtca cgaccgcgaa ggacggcgcc gaggcgctgc
     5461 agcggatcgc cgcggagcgc cccgacctcg tcctgctcga cctgctcatc ccggccccgg
     5521 aaggcttgca ggtgatccgg acggtgaagc gcgaccgcgc catgtccacc gtgccgatcg
     5581 tcgtgatgac ggtcaagcgg gacgtgaaga cgaagatcga ggcgctccgg accggggcgg
     5641 acgacttcat cgtcaagccg ttccacttcg acgagctcga cgcggtcatg cgcgcctcgc
     5701 tcaagaagcg gtacctctac acctcgctcg agcgcgccaa ccgggcgctc cgggacgcca
     5761 acgagaagct gaccaagctg tcggtcaccg atgatcgcac caacctcctc aacgaccgct
     5821 acctgaagcg gcggctgtcg gaggagttca agcgggcacg gcgctacggg gcgccgctca
     5881 ccgtcatgat cctcgacctg gatcacttca agcaggtgaa cgacaagtac ggacacgact
     5941 gcggcgacca
//
LOCUS       DASRIQ010000316         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_130747, whole genome shotgun sequence.
ACCESSION   DASRIQ010000316 DASRIQ010000000
VERSION     DASRIQ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130747"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFD06_07625"
     CDS             <1..603
                     /locus_tag="VFD06_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZN03445.1"
                     /translation="HADRSVVLRFGLPAVAAAFAGAALLAALASRPPVRLVWPWGTAE
                     VPPVQIGMGILILLFAIVELVPERLVALRLPARWLPLGGLLSGFFGGLSGHQGALRAI
                     FLRRAGLNPRAFAGTQSILALLVDAARLLVYGAAAWGRHFGALTSEGAAQPVVVGTLC
                     AFAGAFMGSRLFPKVTIGAVQKLVGLLLIVTGAGLATGLL"
     gene            complement(638..2725)
                     /locus_tag="VFD06_07630"
     CDS             complement(638..2725)
                     /locus_tag="VFD06_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZN03446.1"
                     /translation="MSESTTAPAPRGPIVPARLKPGLTPSDVARKAPQPAGAPASGEA
                     GSPGRVVHGRVLIFWDPARPGQKRDAIDTDQITPAADCVSESLDTLDERWKAGSFRHL
                     MPDFRARVHRGETFLVAGERFAIGSSREMSPAGLKGAAEEAGLEMVVVCGAGMGDIFR
                     RNALNLGLHVAQSPEAVADAKDGDEMAFETGKRILRNLTQGKEYRPVPLTEKEEEIRR
                     GGGIFEVGRAVFPESVSRAPRVEFPDPSTARALTVAEQIYWAHRVDRSARVEPGTTLL
                     LQADLLPGSDGTAPFAIHTFNQITGGNTIFPRQIAIAADHFVFTGKAEDDRQTEISRQ
                     FARLHDLGKPHYAAPGDGIFHFYFPEQGLIVPGGFYPGADSHSRAYGAYGAVGVGIGS
                     TTLGFGWATGSIYFSPAPQRRVTFHGRLQPWVSGKDIVLELLRRWGKAQAQGMGVEFA
                     DPGMQLPMTSRNTIANMMAEGEAQNGLFPQDAITEAWFETRGIALPYPRLTPGAEARY
                     ALDETLDLSLVVPMIAKPFSPGNAYPAEEVAHERLTFDKALIGSCTNGSYDDLLVAAL
                     VLRAADAAGHGRVAQGIEFVVFPGSGTVKRQIEVPEPRLGGRSIADVMRDAGAAIRDS
                     WCGPCFGQGPDALHKGQRAITSFNRNWQNRMGLGGEGYLASPQVVAASALAGYMAPPS
                     ALGLRWDADRFGT"
     gene            2830..>3286
                     /locus_tag="VFD06_07635"
     CDS             2830..>3286
                     /locus_tag="VFD06_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN03447.1"
                     /translation="MVPREEETDRELMRRLQRSGDERALTRLVMRHSGRMLAVAARLL
                     GSRADAEDAVQRAFLRCHTHARDYRPEWTVTTWLYRILTNACIDEIRRRRARPEEPMP
                     ATTPAASGRAGAAAEARIDLKAALTKVPTEARVLLVLRYVEGLSYGELAR"
BASE COUNT          429 a         1157 c         1198 g          502 t
ORIGIN      
        1 cacgccgaca ggagcgtcgt gctgcgcttc ggcctgccgg cggtcgccgc cgccttcgcg
       61 ggggccgccc tgctcgcggc tctcgcaagc cggccgccgg tccgcctcgt ctggccctgg
      121 ggaaccgccg aggtgccgcc ggtgcagatc gggatgggga tcctgatcct cctcttcgcg
      181 atcgtcgagc tggttccgga gcgcctcgtc gcgctgcgac tccccgcccg ctggctgccg
      241 ctcggcgggc tcctgtccgg cttcttcgga gggctctcgg ggcaccaggg ggcgttgcgg
      301 gcgatcttcc tgcgccgcgc cgggctcaat ccgcgcgcct tcgccggcac gcagtcgatc
      361 ctggcgttgc tcgtcgatgc ggcgcgcctg ctggtctacg gcgcggcggc gtggggacgg
      421 cacttcggag cgctcacttc ggagggagcg gcgcaaccgg tcgtcgtcgg aacgctgtgc
      481 gccttcgccg gagccttcat ggggtcgcgt ctgttcccga aggtgacgat cggcgccgtg
      541 cagaaactgg tcgggctgct gctcatcgtc accggcgccg ggctcgcgac cgggcttctt
      601 tgaatcgcgc ggcgctgatc gagccgcagc ggggagccta ggtcccgaag cggtcggcgt
      661 cccagcgcag gccgagggcg gaggggggcg ccatgtagcc ggcgagggcc gacgccgcca
      721 cgacctgagg actcgcaagg tagccctcgc cgccgagccc catccggttc tgccagttgc
      781 ggttgaacga ggtgatggcg cgctgtcctt tgtggagcgc atcgggtccc tggccgaagc
      841 aggggccgca ccacgagtcg cggatggccg cgccggcatc gcgcatgacg tcggcgatcg
      901 agcgccctcc gagccgcggc tcggggactt cgatctgccg cttcaccgtg ccgctgccgg
      961 ggaagaccac gaactcgatc ccctgggcca cgcggccgtg tccggcggcg tcggcggcgc
     1021 gcagcaccag cgccgccacg agcaggtcgt catacgagcc gttggtgcag gagccgatga
     1081 gcgccttgtc gaaggtcagc cgctcgtgcg ccacctcctc ggccgggtac gcgttcccgg
     1141 ggctgaatgg cttggcgatc atcggtacga ccaacgacag gtcgagcgtt tcgtcgaggg
     1201 catagcgcgc ttcggccccg ggcgtcaggc gcggatacgg gagcgcgatg ccgcgcgtct
     1261 cgaaccacgc ctcggtgatc gcgtcctgcg ggaacaggcc gttctgcgcc tccccctccg
     1321 ccatcatgtt ggcgatggtg ttgcgcgagg tcatcggcag ctgcatgccc gggtcggcga
     1381 actccacacc catcccctgg gcctgcgcct tcccccagcg tcgcaggagc tccagcacga
     1441 tgtccttccc ggaaacccac ggctgcaggc gtccgtggaa ggtgacgcgc cgctgcgggg
     1501 cagggctgaa atagatgctg ccggtcgccc agccgaagcc gagcgtggtg ctgccgatcc
     1561 cgaccccgac cgccccgtac gccccgtagg cgcggctgtg cgaatcggcc cccgggtaga
     1621 agcctcccgg aacgatgagc ccctgctccg gaaagtagaa atggaagatg ccgtcccccg
     1681 gggctgcgta gtgcggcttg ccgagatcgt gcaggcgggc gaactgccgc gagatctcgg
     1741 tttgtctgtc gtcttccgcc ttgcccgtga agacgaagtg atcggccgcg atggcgatct
     1801 gccgcgggaa gatggtgttc cctccggtga tctggttgaa ggtatggata gcgaaggggg
     1861 cggtgccgtc cgatcccggc aggagatccg cctgcagcag cagggtcgtg ccgggctcga
     1921 ccctggcgct ccgatccacc cggtgcgccc agtagatctg ctccgccacc gtcagggcgc
     1981 gggcggtcga aggatccggg aactcgacgc gcggcgcgcg ggacaccgac tccggaaaaa
     2041 cggcccgacc cacctcgaag atgccgccgc cgcggcggat ctcctcttcc ttctccgtca
     2101 gcggaacggg gcggtactcc ttcccctgcg tcaggttgcg caggatgcgc ttcccggtct
     2161 cgaacgccat ctcgtcgccg tctttcgcgt cggccaccgc ctcgggactc tgcgccacgt
     2221 gcaggccgag gttgagggca ttgcggcgga agatgtcccc catgccggcg ccgcagacga
     2281 ccaccatctc caggccggcc tcctcggccg cccctttgag ccccgccggc gacatctcgc
     2341 gcgacgagcc gatggcgaag cgctcccccg cgacgaggaa ggtctcgccg cggtggacgc
     2401 gcgcgcggaa gtcgggcatc aggtggcgga aggagccggc cttccagcgc tcgtcgaggg
     2461 tgtcgaggct ctccgagacg cagtcggcgg cgggggtgat ctggtcggtg tcgatggcgt
     2521 cgcgcttctg tcccggccgc gccgggtccc agaagatcag gacgcggccg tgcaccacgc
     2581 ggcccggcga cccggcctcc cccgatgcgg gcgcgccggc cggctgcggc gccttgcgcg
     2641 ccacgtcgga gggcgtcagc ccgggcttca gacgcgcggg aacgatcggt cctcggggcg
     2701 ctggggctgt cgtgctctca ctcatgggag cagcattgta acgcgggcgc gatcgcgccg
     2761 gcctgccgcg gggccgccgc ccggaatcgc ctccacccgt tccctcttcc cccaccgtat
     2821 acactcgtga tggtgccacg cgaagaggaa acggatcggg agctgatgcg ccggctgcag
     2881 cgctcggggg acgagcgggc gctgacgcgc ctggtgatgc ggcactcggg gaggatgctg
     2941 gcggtcgcgg cgcgcctgct cggctcgcgc gccgacgccg aggacgcggt gcaacgggcg
     3001 ttcctgcgct gccacacgca tgcgcgcgac taccggcccg agtggacggt gacgacctgg
     3061 ctgtatcgca tcctaacgaa tgcctgcatc gacgagatcc ggcggcggcg agctcgcccg
     3121 gaagagccga tgccggccac gacccccgcc gcgtccgggc gggcgggcgc ggccgcggag
     3181 gcccgtatcg atctgaaggc ggcgctcacg aaggtgccga ccgaggcgcg ggtgctcctg
     3241 gtgctgcgct acgtcgaagg actgtcgtac ggcgagctgg cgcggg
//
LOCUS       DASRIQ010000317         5263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3247052, whole genome shotgun sequence.
ACCESSION   DASRIQ010000317 DASRIQ010000000
VERSION     DASRIQ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3247052"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..>5263)
                     /locus_tag="VFD06_07640"
     CDS             complement(23..>5263)
                     /locus_tag="VFD06_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HZN03448.1"
                     /translation="RSDTFNGHTIHSLKSDYQTRPNGGDGWLRIMTFSPANNTIQVQT
                     YSPTLGQFINSHADNTAGTAQNEFTLTYEMAGGTPFVTLGNDSEPSGGTVCVPWPGRQ
                     PGRQYEWSVAVSDGTSTTTSPRWTFTTAQNCTLDSECADGNLCNGVETCTGNTCQPGT
                     PPDCDDVDACTTDSCNPASGCTHAPVSCDDGDACTTDSCDQISGCAHAPVTCPQGQVC
                     TPATGLCAEPAATCVSAAECGDGNACTTDTCVGANVSALSLDGSNDYVTMGTAPGLGS
                     ATFTVELWFKKTGDGIPNTTSGTNGIASLLPLVAKGAPEEDGSNLDANYIVGIDTAGN
                     FLAADFEDAASGSNHPITASITPIVNGVWYHAAATYDGGTWRLYLNGNLEATLGVGAF
                     TPRADSVQPFGIGAMQTSTGTRLGAFQGLVDEVRVWNRALSLEEIQAGRDLELTGGAG
                     LVGRWGLNENGGASAGDSAGAGPNAENGTLTNGPGWSAADRAPLASGLCAHAPAPGCV
                     PCNGDEECLDANVCNGVETCSGGVCQPGTTLSCDDGDACTVDACDAEDGCQHGVVSCD
                     DGVACTADACDPGLGCSHLGTCAPGSICDPGSGQCEARCATAADCNDNSACTTDTCVG
                     GNVAALNFNDATDDFVTMGQAAGENALGARAFTLEAWIRRDGASWGSTTGTGTGGVTA
                     VPLITKGRGEQEESNVDMNYFLGITATGRPVADFEQFAAGGGWAAGQNHPACSSASIA
                     DQAWHHVAVTYSAAAGWHFYIDGVEGTTTDGTTCTTCSPAGSCPRSPGVEPRYDSIQH
                     FGLGTAMTSAGTTGAAGSYAGVMDEARVWSRALGQAEIAANRGLPLTSGVDLIGRWGF
                     EDGTADDSTTPAEDGTLVNAPVFSLSDKPALQSGTCAFTPIPCSDGNVCTSDACNPAT
                     GCVFPPANQGVACDDGSACTSGNACSDGVCSGATNAALCDDANTCSTDVCAAAPPQGA
                     LAFDGANDFVDMGAAAELNGTRFTIETWFKKTGDGIPNTTSATNGIASLLPLVTKGAP
                     EADGSNLDANYIFGINTAGNFLAADFEDTATGSNHPITAAITPIVNDTWYHAAATYDG
                     TTWRLYLNGALEATAVVGPVTPRADSIQHAALGAMVTSTGSRLGAFQGVLDEVRIWNR
                     ARSQAEIQAGVTQRITSSPGLVGRWGLDDASGTNAVDSTVPASNGTLTNFTLPGPWVT
                     GAPALADVLCSSGSQPDGTSCPDDGNACTTNACGLGVCGATYAPAPGCCAQDAQCDDA
                     NNTTVDTCVAASCNNAAPVACNAAAQCDDANTCSLDACVGGNVSALNFNGTSDFVTMG
                     QAAGENALGARAFTLEAWIRRDAASWGTTTNTGTGGVTGVPIVTKGRGEAENSNVDAN
                     YFFGITATGRPVADFEQFAAGGGWAAGQNHPGCSSASISDQNWHHVAVTYSTTTGWRF
                     YIDGVEGTTADGTACTTCSPAGSCPQNPAVEPRYDSVQHFGLGTAMTSAGTSGAAGFF
                     GGVLDEVRVWKRARAVAEIQAGRTQEIATDTDLIGRWSLNENSGTVATDTTATPTQNN
                     GNFNGAPAWSAADQAPLLAGTCTHTAGSTGAMCRAAGPVCDVAETCTGASVFCPSDLK
                     ATNGTACSDGNACTEADSCQAGACSGAPIPAPGEVANVTFDLAGGTLTWSALGGALPI
                     QYDVTRGLTISPPTGGAPGSVCVATATSLTEASDATLPGSGEAYWYLVRGRHSCGTGT
                     WGYQAQDGVPGLERLVNACP"
BASE COUNT          619 a         1844 c         1945 g          855 t
ORIGIN      
        1 cggtcgcccg gtgacccggc gatcagggac aggcgttgac gagccgctcg agtcccggaa
       61 cgccgtcctg cgcctggtag ccccaggtcc cggtgccgca cgaatggcgg ccgcggacga
      121 ggtaccagta ggcttcccca gaacccggca acgtcgcatc gctcgcttcg gtgaggctcg
      181 tcgcggtcgc cacgcagacg gaaccgggcg cgccgccggt cggcggactg attgtcagtc
      241 cgcgggtgac gtcgtactgg atcggcaggg ccccgcccag cgcgctccag gtgagcgtcc
      301 ctcccgccag gtcgaaggtg acattggcca cttcccccgg cgccgggatc ggcgccccgc
      361 tgcacgcccc cgcctggcag gagtcggcct ccgtgcaggc attcccgtcg ctgcacgcgg
      421 tgccgttcgt cgccttgaga tccgacgggc agaagacgct cgccccggtg cacgtctcgg
      481 cgacatcgca gacgggaccg gcggcccggc acatcgcgcc ggtgctcccg gcggtgtgcg
      541 tgcaggtgcc ggcgaggagc ggagcctggt cggccgcgct ccaggcgggg gcgccgttga
      601 agttgccgtt gttctgcgtg ggcgtggcgg tcgtgtccgt cgcgaccgtg ccgctgttct
      661 cgttcaggct ccatcggccg atgaggtcgg tgtcggtcgc gatctcctgc gtgcggccgg
      721 cctggatctc cgcgaccgcg cgggcacgct tccagacgcg cacctcgtcc aggacgccgc
      781 cgaagaagcc ggcggcgccg ctcgtgcccg ccgaggtcat cgccgtgccg aggccgaagt
      841 gctggacgct gtcgtagcgc ggctcgaccg ccgggttctg ggggcaggag ccggccgggc
      901 tgcaggtggt gcacgccgtg ccgtcggcgg tcgtcccctc cacgccgtcg atgtagaaac
      961 gccagccggt cgtggtgctg taggtcaccg ccacgtggtg ccagttctgg tcgctgatcg
     1021 aggcgctgct gcatccgggg tggttctggc ccgcggccca gccgccgccc gcggcgaact
     1081 gctcgaagtc ggccaccggg cggccggtgg cggtgatgcc gaagaagtag ttggcgtcca
     1141 cgttggagtt ctcggcttcg ccgcgcccct tggtgacgat cgggacgccg gtgaccccgc
     1201 cggtgccggt gttggtggtc gtgccccatg acgcggcgtc gcgccggatc caggcttcga
     1261 gcgtgaaggc ccgcgcgccg agcgcgttct ccccggccgc ctgccccatc gtcacgaaat
     1321 cggaggtgcc gttgaagttg agcgccgaga cgttgccgcc gacgcaggcg tcgaggctgc
     1381 acgtgttcgc gtcgtcgcac tgcgccgccg cgttgcacgc caccggcgcg gcgttgttgc
     1441 aggaggccgc gacgcaggtg tccacggtcg tgttgttggc gtcgtcgcac tgcgcgtcct
     1501 gcgcgcagca gccgggtgcc ggcgcgtagg tcgcgccgca cacgccgaga ccgcacgcgt
     1561 tggtggtgca ggcgttgccg tcgtcggggc aggaggtccc gtccggctgg ctgcccgagg
     1621 agcagaggac gtccgcgaga gccggggcgc ccgtcaccca gggtcccggc agggtgaagt
     1681 tcgtgagcgt cccgttgctg gctggaacgg tggaatcgac ggcgttcgtg ccggaggcgt
     1741 catccagccc ccaccggccg acgagccccg gactcgaggt gatgcgctgc gtgacgccgg
     1801 cctggatctc cgcctggctg cgggcccggt tccagatgcg cacttcgtcg agcaccccct
     1861 ggaacgcgcc gagccgcgat ccggtggagg tgaccatcgc gccgagcgcc gcgtgctgga
     1921 tgctgtcggc gcgcggcgtg accggcccga cgacggcggt cgcttcgagc gcgccgttca
     1981 ggtagagccg ccaggtcgtg ccgtcgtacg tcgcggcggc gtgataccag gtgtcgttga
     2041 cgatgggggt gatcgcggcg gtgatcggat ggttgctgcc ggtcgccgtg tcctcgaagt
     2101 cggcggccag gaagttcccg gccgtgttga tgccgaagat gtagttggcg tccaggttcg
     2161 agccgtccgc ctccggcgcc cccttggtca ccagcggcag gagcgacgcg atgccgttcg
     2221 tggcgctcgt cgtgttcggg atgccgtctc cggtcttctt gaaccaggtc tcgatggtga
     2281 accgggtgcc gttcagctcg gcggcggccc ccatgtccac gaaatcgttc gcaccgtcga
     2341 aggccagcgc cccctgcggc ggcgccgcgg cgcagacgtc ggtgctgcag gtgttggcgt
     2401 cgtcgcacag ggccgcgttg gtcgcgccgc tgcagacccc gtcgctgcac gcgttccccg
     2461 atgtacaggc gctgccgtcg tcgcacgcga ccccctggtt cgccggcggg aagacgcatc
     2521 cggtcgcggg gttgcacgca tcgctcgtgc acacgttgcc gtcgctgcag gggatcggcg
     2581 tgaaggcgca ggttcccgac tgcagcgccg gcttgtcgga gagggagaag accggagcgt
     2641 tgaccagcgt cccatcctcg gccggggtcg tcgagtcgtc ggccgtgccg tcctcgaagc
     2701 cccaccggcc gatgagatcg acgccgctcg tcagggggag ccccctgttc gcggcgatct
     2761 ccgcctggcc gagcgcccgg ctccacacgc gggcctcgtc catgacgccg gcataggagc
     2821 cggccgctcc cgtcgtcccg gccgaggtca tcgccgtgcc gagcccgaag tgctggatgc
     2881 tgtcgtagcg cggctcgacc ccggggctcc gcgggcacga gccggccggg ctgcacgtgg
     2941 tgcaggtggt gccgtcggtc gtcgtccctt ccacaccgtc gatgtagaag tgccagccgg
     3001 cggcggcgct gtaggtgacc gccacgtggt gccaggcctg gtcggcgatc gaggcgctgc
     3061 tgcacgccgg atgattctgc ccggcggccc acccgccgcc cgccgcgaac tgctcgaagt
     3121 cggccaccgg acgaccggtc gcggtgatgc cgaggaagta gttcatgtcg acgttcgact
     3181 cctcctgctc gccgcgcccc ttggtgatga gcggcacggc ggtgacgccg ccagtgccgg
     3241 ttccggtcgt ggatccccat gaggcgccgt cgcgccggat ccaggcttcg agcgtgaagg
     3301 cccgggcgcc gagggcgttc tcccccgccg cctggcccat ggtgacgaag tcgtcggtcg
     3361 cgtcgttgaa gttcagcgcc gcgacgttgc cgccgacgca ggtgtcggtc gtgcaggcgc
     3421 tgttgtcgtt gcagtcggcc gcggtcgcgc agcgcgcctc gcactggccg ctccccgggt
     3481 cgcagatgct tcccggcgcg caggtgccga ggtgcgagca gccgagcccg gggtcgcagg
     3541 cgtcggcggt gcaggcgaca ccgtcgtcgc acgacacgac gccgtgctgg cagccgtcct
     3601 ccgcgtcgca cgcatcgacc gtgcaggcgt cgccgtcgtc gcacgacagc gtcgtccccg
     3661 gctggcagac gccgccggag caggtctcga cgccgttgca cacgttcgcg tcgaggcact
     3721 cctcgtcgcc gttgcacggg acgcagcccg gagccggcgc gtgcgcgcac aggcccgagg
     3781 cgagcggcgc gcgatccgcc gcgctccagc ccggcccgtt ggtcagcgtg ccgttctcgg
     3841 cgttcggccc cgctcccgcg gagtcgccgg cgctcgcgcc gccgttctcg ttcaggcccc
     3901 accgtccgac caggccggcc ccgcccgtga gctcgagatc gcgcccggcc tggatctcct
     3961 ccaggctgag ggcccggttc cagacgcgca cttcgtcgac gaggccctgg aatgctccga
     4021 ggcgtgtgcc ggtcgaggtc tgcatcgccc cgatcccgaa cggctggacg ctgtcggcgc
     4081 gcggcgtgaa cgcccccacc ccgagggtcg cctcgaggtt gccgttcagg tacaggcgcc
     4141 aggtgccgcc gtcataggtc gccgcggcgt ggtaccagac gccgttgacg atcggagtga
     4201 tcgaagcggt gatcggatgg ttgctgccgg acgccgcgtc ctcgaaatcg gccgccagga
     4261 aattccccgc cgtgtcgatg ccgacgatgt agttcgcgtc caggttcgag ccgtcctcct
     4321 ccggcgcccc cttcgccacg agcggcagca gcgaggcgat gccgttggtt ccgctggtgg
     4381 tgttggggat gccgtccccg gtcttcttga accacagctc caccgtgaag gtcgcgctgc
     4441 cgagccccgg ggccgttccc atggtgacgt agtcgttgct gccgtcgagc gacagggcgg
     4501 agacattcgc cccgacgcag gtgtcggtcg tgcaggcgtt cccgtcgccg cactcggcag
     4561 cggagacgca ggtcgcggcc ggctcggcgc agaggccggt cgccggggtg cacacctgtc
     4621 cctgcgggca ggtgaccggc gcgtgcgcgc agccggagat ctggtcgcag ctgtcggtcg
     4681 tgcaggcgtc gccgtcgtcg caggagaccg gcgcgtgcgt gcagccgctg gccgggttgc
     4741 aggaatcggt cgtgcaggcg tcgacgtcgt cgcagtccgg cggcgtgccg ggctggcagg
     4801 tgttgccggt gcaggtctcg acgccgttgc acaggttgcc gtcggcgcac tcgctgtcga
     4861 gcgtgcagtt ctgcgcggtg gtgaaggtcc agcgcggcga ggtggtcgtc gacgtgccgt
     4921 cgctcaccgc caccgaccac tcgtactgcc ggcccggctg gcgccccggc cacggcacgc
     4981 acacggtgcc gccgctcggc tcggagtcgt tgccgagcgt cacgaacggc gtgccgcccg
     5041 ccatctcgta cgtcagggtg aactcgttct gcgccgtccc cgccgtgttg tccgcgtggc
     5101 tgttgatgaa ctgcccgagc gtcggcgaat acgtctgcac ctggatggtg ttgttggccg
     5161 gggagaaggt catgatgcgc agccagccgt cgccgccgtt cggccgcgtc tggtagtccg
     5221 acttgagcga gtggatcgtg tgcccgttga acgtgtcgga tcg
//
LOCUS       DASRIQ010000318         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3520297, whole genome shotgun sequence.
ACCESSION   DASRIQ010000318 DASRIQ010000000
VERSION     DASRIQ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3520297"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="hflC"
                     /locus_tag="VFD06_07645"
     CDS             <1..835
                     /gene="hflC"
                     /locus_tag="VFD06_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HZN03449.1"
                     /translation="QFGRPVGGVIATPGLHLKTPFVQEVHRFDKRWLEYDGDPNEIPT
                     RDKKYIWVDTYARWRIADPLRFYQAVKDEIGGQSRLDDIVDGQTRYAIASFDLIEAVR
                     STNREFELTEELQGIGSEEARARITTGRAEIEKIILEKAAKITPEFGIELVDVRIKRL
                     NYVESVQQKVFERMISERKRIAERSRSEGQGRAAEILGEKEREVRSATSAGYRQAQEL
                     KGKADAQAIAITAKAFGRDPDLYQFLKSLETLNATVDKETWLILSTDSEVLKFLKGAS
                     R"
     gene            1059..2375
                     /locus_tag="VFD06_07650"
     CDS             1059..2375
                     /locus_tag="VFD06_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HZN03450.1"
                     /translation="MALGLGLAVVAFADEAFNDLVKRLGGEKGKFAERQQTMLSERYD
                     LADRAAPGAKMSRGKPVQGGVRVRLPQGMTWARLATMTPEDIKAKGVWPKGFFPMPHP
                     HHEAGGMIFPQPTIDEIKKQTQRDITRFDLDFDLPQHLLPEFPPAIFLTTRPDLGDVS
                     KGRLITSHNFRDTFKDVLNPKQLEGLRLLVTPFPQQQFNATDDRRSLQPSLGVACFDC
                     HVNGHTNAATHTVGDIRPDEHRHRIDTPSLRGVNIQRLFGSQRAMKSVEDFTEFEQRA
                     AYFDGDPEAATRKGVNILERGSQVHFMAEFQDLLDFPPAPKLDVFGRLDPAKATEEEL
                     RGEQLFNGKGRCATCHTPPYYTDNLMHNLRVERFYNETVINGRKASADGPIKTFPLRG
                     IRESPPYLHDDRLLTLEDTVEFFNLVLGTKLTDQEKKDIAAFLRTL"
     gene            2389..2754
                     /locus_tag="VFD06_07655"
     CDS             2389..2754
                     /locus_tag="VFD06_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03451.1"
                     /translation="MIAWEDVEPYLRDDVVLRAEHAVLIAVLAVAILGAIRRKIRRAF
                     GIKHLKVARGDKSMTILYVAYALLTVAVTLTVQVADSAQGYKVGLIVFDYLALAYLFF
                     FNSWFRNRLLRLLGRVYED"
BASE COUNT          501 a          981 c          967 g          429 t
ORIGIN      
        1 ccagttcggc cgcccggtcg gcggcgtcat cgcgaccccg gggctgcacc tcaagacgcc
       61 gttcgtccag gaggtgcacc ggttcgacaa gcgctggctg gagtacgacg gcgacccgaa
      121 cgagatcccg acgcgcgaca agaagtacat ctgggtggac acctacgccc gctggcgcat
      181 cgccgacccg ctgcgcttct accaggcggt gaaggacgag atcggcggcc agagccggct
      241 cgacgacatc gtggacgggc agacccgcta cgccatcgcc tcgttcgacc tcatcgaggc
      301 ggtccgctcc accaaccggg agttcgagct caccgaggag ctgcagggga tcggctcgga
      361 ggaggcgcgg gcccgcatca ccaccggccg cgccgagatc gagaagatca tcctcgagaa
      421 ggcggcgaag atcaccccgg agttcggcat cgagctcgtg gacgtgcgca tcaagcggct
      481 caactacgtc gagagcgtgc agcagaaggt gttcgagcgg atgatctccg agcgcaagcg
      541 catcgccgag cgcagccggt cggaggggca gggtcgcgcc gcggagatcc tcggcgagaa
      601 ggagcgcgag gtgcgctccg ccacgtccgc gggctaccgg caggcgcagg agctcaaggg
      661 gaaggcggac gcgcaggcga tcgccatcac ggcgaaggcc ttcggccgcg accccgacct
      721 ctaccagttc ctcaagtcgc tcgagacgct caacgccacc gtcgacaagg agacctggct
      781 gatcctgtcg accgattccg aggtgctgaa gttcctcaag ggagcgtcgc gatgaccggg
      841 gatctcgatc cggcggggat tctccagctg gggggctggg gctgcacgcg cggagtgcgc
      901 acgacttctt cgacgcgctg gtcgcgctcg gcatgctgga gaagaaggac gggcgcggcg
      961 gtaggaatca agtaggtcgg gagggtagaa tccccgcctc gaaggaggga acgcccgtga
     1021 acgttcgcgc ccgttttccc ctcgctctcg tgtccgccct ggcgctgggc ctcggcctcg
     1081 ccgtcgtcgc cttcgccgat gaagccttca acgacctcgt gaaacgcctc ggcggcgaaa
     1141 aggggaagtt cgccgagcgg cagcagacga tgctctccga gcgctacgac ctcgccgatc
     1201 gcgcggcccc gggggcgaag atgtcccgcg gcaagccggt gcaggggggc gtccgggtcc
     1261 ggctgccgca ggggatgacc tgggcccgcc tcgcgaccat gacgccggag gacatcaagg
     1321 cgaagggggt ctggccgaag gggttcttcc cgatgccgca cccgcaccac gaggcggggg
     1381 ggatgatctt cccgcagccg accatcgacg agatcaagaa gcagacgcag cgggacatca
     1441 cccgcttcga cctcgacttc gacctgccgc agcacctgct gccggagttc ccgccggcga
     1501 tcttcctgac gacgcggccg gatctcggcg acgtgtcgaa ggggcggctc atcacgtctc
     1561 acaatttccg cgacacgttc aaggacgtcc tcaacccgaa gcagctcgag gggctgcgtc
     1621 tcctcgtgac gccgttcccg cagcagcagt tcaacgccac cgacgaccgg cgcagcctgc
     1681 agccgtcgct cggggtcgcc tgcttcgact gccacgtcaa cggccacacc aacgccgcca
     1741 cccacacggt cggcgacatc cggccggacg agcaccgcca ccgcatcgac acgccgtcgc
     1801 tgcggggggt caacatccag cgcctgttcg ggtcgcagcg cgccatgaag tcggtcgagg
     1861 acttcaccga gttcgagcag cgggccgcct acttcgacgg cgaccccgaa gcggcgacgc
     1921 ggaaaggggt gaacatcctg gagcgcggca gccaggtcca cttcatggcg gagttccagg
     1981 acctcctcga cttcccgccg gcgccgaagc tcgacgtctt cggccggctc gatccggcga
     2041 aggcgaccga agaggaactg cgcggcgagc agctcttcaa cggcaaaggc cgctgcgcca
     2101 cctgccacac gccgccgtac tacaccgaca acctgatgca caacctccgg gtcgagcgct
     2161 tctacaacga gacggtgatc aacgggcgca aggcgtcggc cgacggcccg atcaagacct
     2221 tcccgctgcg cggcatcagg gagtcgccgc cgtacctgca tgatgatcga ttgttgacgc
     2281 tcgaggacac ggtggagttc ttcaacctcg tcctcgggac gaagctcacc gaccaggaga
     2341 agaaggacat cgccgccttc ctgcggacgc tttgatccct gccggctctt gatcgcctgg
     2401 gaggacgtcg aaccgtatct gcgcgacgat gtcgtgctgc gcgcggagca cgccgtcctc
     2461 atcgcggtgc tggccgtggc gatcctcggg gcgatccgcc ggaagatccg ccgggccttc
     2521 gggatcaagc atctcaaggt ggcgcgcggg gacaagagca tgaccatcct ctacgtcgcc
     2581 tacgcgctcc tcaccgtggc ggtcacgctc acggtgcagg tggcggattc cgcacagggg
     2641 tacaaggtcg ggctcatcgt gttcgactac ctggcgctgg cctacctgtt cttcttcaac
     2701 tcctggttcc gcaaccggct gctgcgcctg ctcggccgcg tctacgagga ttgaggagcg
     2761 catgaacggc gacatggcct ggctctggtg ggcgccgctc atcgccgcct gtctgcacat
     2821 cttcgaggag ttcgtgtggc cgggcggctt cgcggagtgg gaccgcgcct accgcccg
//
LOCUS       DASRIQ010000319         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1950053, whole genome shotgun sequence.
ACCESSION   DASRIQ010000319 DASRIQ010000000
VERSION     DASRIQ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950053"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /gene="rpe"
                     /locus_tag="VFD06_07660"
     CDS             <1..553
                     /gene="rpe"
                     /locus_tag="VFD06_07660"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZN03452.1"
                     /translation="VPNLTIGPPVVASIRKTTTLPLDCHLMIEEPDRYIERFVEAGAD
                     MISVHQETVPHLHRTVHLIRERGAGCGVALNPATPIATLEDILPDLDYVLLMSVNPGF
                     GGQRFLPMVIPKIRALRERIAARGLRTRIEVDGGVTGENLPALLEAGATLFVAGSAVF
                     DGKDPRARARALVDLMGRAPGVA"
     gene            565..963
                     /locus_tag="VFD06_07665"
     CDS             565..963
                     /locus_tag="VFD06_07665"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN03453.1"
                     /translation="MARSSKVIVRVRYPEVDRMGVVHHRNHFVWFEIGRTELMREAGL
                     PYAGVEDGGLYLPVIEASCSYHSPARYDEELQVTTTLEKVSPVRATFGYRLERKADGR
                     LLASGTTTHAAIDGRGRPRRLPDALRRLLV"
     gene            963..1838
                     /gene="bamD"
                     /locus_tag="VFD06_07670"
     CDS             963..1838
                     /gene="bamD"
                     /locus_tag="VFD06_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HZN03454.1"
                     /translation="MPPSCPARRPRLRALVAALVVVVAASFVSPAWAKKDKKAVLPPQ
                     EAYELAMQKMAKKRYYQARTMLQEILPRVAPEDRELLPKVQLGIADAFFKDGGMLNYG
                     EALNAYRNFLTYFPQHDQAAYAQFMIGQSMYRQVLSPDRDQTMTLKAIDELKKLEASH
                     PNSPYAEGARQTIEICRARLAEKERMVGRFYQRRKSYLAAIDRYRYALDHYPSAASSG
                     RLLYDLGRCLLSVNRKEEADEVFAKLQQAPDHDDWVRKAEAAVRDYDKRREKEGEKIF
                     GELGKDETAKKKGTP"
     gene            1835..2743
                     /locus_tag="VFD06_07675"
     CDS             1835..2743
                     /locus_tag="VFD06_07675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN03455.1"
                     /translation="MSRAATASQRPLSVIIPTFNEEETIEDCLASVAFADEILVVDSF
                     STDRTVDIAKARGARVVQREYGYSAQQKNWAIPQARHEWVLLVDADERVSAPLRDEMR
                     DLLSRGPEADGYWIRRANHFLGKRIRFCGWGSDKVIRLFRRDAARYQDRQVHAEIDLA
                     GPLPMLRHPLEHHTFRSWSQYWRKLDLYSEWGARQMFLEGRRTSGVQILWRPIGRFIR
                     MYVLRLGFLEGTHGVVLSMLGAFTVYLKHARLWEMQRAGTAAREAAGPEPPGGGAAAA
                     SAGEAGPLLREQIGRMVDEDRARKAR"
     gene            2748..>3322
                     /locus_tag="VFD06_07680"
     CDS             2748..>3322
                     /locus_tag="VFD06_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN03456.1"
                     /translation="MASTGPALLQLGTEPGWRGGEAQVLFLAEGLRRRGRRPVVAAPP
                     GSALLERASEAGLETVPFAARGEWDLAAGRRLARLARGLPASLVHAHTAHAAGLCLLA
                     RPLGLRSRVVLARRVSFPLRPPGLGRLKYSLGVDRIVAVSEAIRRSLLAQGLAADRVV
                     TVHSGIDAARFAAGRREPARGGLLRQGAFPA"
BASE COUNT          474 a         1152 c         1219 g          477 t
ORIGIN      
        1 cgtcccgaat ctgacgatcg gcccgccggt cgtcgcctcg atccgcaaga cgaccacgct
       61 gccgctcgac tgccatctca tgatcgagga gccggaccgc tacatcgagc ggttcgtcga
      121 ggcgggcgcg gacatgatct cggtgcacca ggagaccgtg ccgcacctgc accggacggt
      181 ccacctcatc cgcgagcgcg gagcgggctg cggcgtggcg ctgaacccgg cgacgccgat
      241 cgcgacgctc gaagacatcc tcccggatct cgactacgtg ctgctcatgt cggtcaaccc
      301 gggattcgga ggtcagcggt tccttccgat ggtgatcccc aagatccggg cgcttcggga
      361 gcggattgcc gcccgcgggc tccggacgcg catcgaggtg gacggtgggg tgacgggcga
      421 gaatctgccg gcgctcctcg aggcgggggc gacgctgttc gtcgccggat cggccgtctt
      481 cgacggcaag gacccgcgcg cccgcgcgag ggcgctcgtc gatctcatgg ggcgggcccc
      541 cggggtcgcg tgacgggacc ggggatggcg aggtcctcga aggtgatcgt gcgggtgcgc
      601 tatcccgagg tcgaccgcat gggggtggtg caccaccgca atcacttcgt ctggttcgaa
      661 atcggccgca ccgagctgat gcgtgaggcc gggctgccgt atgccggcgt cgaggacggg
      721 ggactctacc tgccggtcat cgaggcatcc tgctcctatc attcgccggc gcgctacgac
      781 gaagagctcc aggtgacgac gacgctcgag aaggtctcgc cggtgcgcgc cacgttcggc
      841 taccggctgg agcgcaaggc cgacggccgg ctcctggcct cggggacgac gacgcacgcg
      901 gcgatcgacg gccgcggccg gccgcggcgt ctcccggacg ccctgcgccg gctgctcgtc
      961 tgatgccgcc gtcctgtccc gcacgccgcc cgcggctccg ggccctggtc gccgcgctcg
     1021 tggtcgtcgt ggcggcctcc ttcgtctccc ccgcctgggc gaagaaggac aagaaggccg
     1081 tgctgcctcc ccaggaagcc tacgagctgg ccatgcagaa gatggcgaag aagcgctact
     1141 accaggcccg cacgatgctg caggagatcc tgccgcgggt cgcgccggag gatcgcgagc
     1201 tcctccccaa ggtgcagctc ggaatcgccg acgccttctt caaggacggc ggcatgctga
     1261 actacggcga ggcgctcaat gcctaccgca acttcctcac ctacttcccg cagcacgatc
     1321 aggcagccta cgcccagttc atgatcgggc agtcgatgta ccggcaagtc ctctccccgg
     1381 atcgcgacca gacgatgacc ctcaaggcca tcgacgagct gaagaagctc gaggcgagcc
     1441 atccgaacag tccgtacgcc gagggggcgc gccagaccat cgagatctgc cgcgcccggc
     1501 tcgccgagaa ggagcgcatg gtcgggcgct tctaccagcg ccgcaagtcg tacctcgcgg
     1561 cgatcgatcg ctaccgctac gccctggatc actacccgtc cgccgcctcg tccggccgtc
     1621 tgctgtacga cctcggccgg tgcctgctct cggtcaaccg gaaggaggag gcggacgagg
     1681 tgttcgcgaa gctgcagcag gcccccgacc acgacgactg ggtgcgcaaa gccgaggcgg
     1741 cggtgcgcga ctacgacaag cggcgcgaga aggaagggga gaaaatcttc ggcgagctgg
     1801 gcaaggacga gacggcgaag aagaagggga cgccgtgagt cgggccgcga ccgcgtcgca
     1861 gcgtccgctg agcgtgatca tcccgacgtt caacgaggag gagacgatcg aggattgcct
     1921 cgcctcggtg gcgttcgccg acgagatcct cgtggtcgac tcgttcagca cggaccggac
     1981 cgtcgacatc gcgaaggcgc ggggggcgcg cgtcgtgcag cgggagtacg gctattcggc
     2041 gcagcagaag aactgggcga tcccgcaggc ccgccacgag tgggtgctgc tggtggacgc
     2101 cgacgagcgg gtctccgcgc cgctgcgcga cgagatgcgg gatctccttt cgcggggccc
     2161 ggaggcggat ggctactgga tccgccgagc caaccatttc ctggggaaga gaatccgctt
     2221 ctgcggctgg gggagcgaca aggtcatccg gctcttccgg cgcgacgcgg cccgctatca
     2281 ggatcgccag gtgcacgcgg agatcgatct cgccgggccg ctgccgatgc tgcgccaccc
     2341 gctcgagcac cacactttcc ggtcctggag ccagtactgg cgcaaactcg acctgtacag
     2401 cgagtgggga gcgcggcaga tgttcctcga ggggcggcgc acgagcggag tgcagatcct
     2461 ctggcgcccg atcggacggt tcatccgcat gtacgtcctg cgtctgggat tcctcgaggg
     2521 gacgcacggc gtggtcctgt cgatgctcgg cgcgttcacc gtctacctga agcatgcccg
     2581 gctgtgggag atgcagcgcg ccgggactgc ggcgcgcgag gccgccgggc cggagccccc
     2641 gggcggcggg gccgctgcgg cctcggcggg ggaggccggt ccgctgctcc gcgagcagat
     2701 cggccggatg gtggacgagg atcgcgcccg caaggcccgc tgacgccgtg gcgtccaccg
     2761 gcccggcgct cctgcagctc gggaccgagc ccggctggcg cggcggcgag gcgcaggtcc
     2821 tgttcctcgc ggaggggctg aggcgccgcg gccggcggcc cgtggtcgcc gccccgcccg
     2881 gaagcgcgct cctggagcgc gcctcggagg ccggcctcga gacggtgccc ttcgcggcgc
     2941 gcggcgagtg ggatctcgcc gccgggcgcc gcctcgcgcg gctggcgcgc ggcctgccgg
     3001 cctccctcgt gcacgcccac accgcccacg ccgccggact gtgtcttctg gcgcggccct
     3061 tggggctgcg gtcgcgcgtc gtcctggcgc gccgggtgtc gtttccgctg cggccgccgg
     3121 ggctcggccg cctcaagtat tcgctcggcg tcgatcggat cgtcgcggtg agcgaggcga
     3181 tccggcggtc gctcctggcg cagggactcg cggcggaccg ggtggtcacg gtgcactccg
     3241 ggatcgacgc cgcgcgcttc gccgcgggcc gccgcgagcc ggcccgcggc gggctgctgc
     3301 ggcagggggc gttcccggcg ga
//
LOCUS       DASRIQ010000320        11146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1442812, whole genome shotgun sequence.
ACCESSION   DASRIQ010000320 DASRIQ010000000
VERSION     DASRIQ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11146
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442812"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VFD06_07685"
     CDS             complement(<1..1024)
                     /locus_tag="VFD06_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN03457.1"
                     /translation="MSNLDAITAQYLSRTRKSREAHDRAKSLLPRGVSSNFRAVDPNP
                     IFVETAVGTTITDLDGNRYIDFGMAFGAMMVGHAHPKIVAAVERQVKRGTLHAMPHRL
                     EAVVAEELRARFGQDQWRFANSGTEATMHAIRLARGATGRPGVVKFEGAYHGAHDTVL
                     VSTKPPLGRVGLREAPASVPASEGIPPGTLQHTHVAQFNDLDSVRRRFEENLNRVACV
                     IVEPIMLNMGVTEPEPAFLPGLREVCDRYGALLIFDEVKTGVKIARGGATEHYKVQPD
                     ITVLAKAIGGGMPLAAFGASADIMEELNSLRVIHVGTYASNPVTLAAAEVTLKEILTD
                     EAYRKVF"
     gene            complement(1075..1926)
                     /locus_tag="VFD06_07690"
     CDS             complement(1075..1926)
                     /locus_tag="VFD06_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03458.1"
                     /translation="MRRLARALSAASVAVAFLGGCDAPHEQDPIVARVHLATRDEGAR
                     KAVAALLSSSGGYEVWQGMREVEYEYRFRFYGGDPTPRLTRSQRHWLSLDDEVHVKLQ
                     DLDGPQPLEVRLDGDQVALSRAGAAVDDPDQADFERAFGRQVRWEFLTPWTLLQPGIL
                     LESRGVRTPPAVGKVPAGPCDVVRVRFERPMEGGSTDDWHDVYISGRSHLVEEILSYR
                     SASNQYRLSVWSDQKPFDGLRVATRRETYASDEGAAVGRLEAVAEYSEVRFIPRDGGG
                     GAPDAGR"
     gene            complement(1923..2792)
                     /locus_tag="VFD06_07695"
     CDS             complement(1923..2792)
                     /locus_tag="VFD06_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03459.1"
                     /translation="MRQSSFVMAAAACVGLLAAPASLVRAQAAGAPPAAARPTDAELQ
                     AITDGGKRIAAYHEAVARCRAALAQRQPDLGEAPGVMVERNGVMHVFLLRREEVGKDT
                     RGLVAVADATWQPRAGEVASLVLLDQSKSPPADVIGHLRALAAARSAAAARAGGPPAP
                     YDEAVFREKGAVAYVVYLQTRPDRTDAARFGSDLKLRLSGDGMQVGEMLPLHDATSLV
                     AVPERPGASPTLHSHAQGDLPTETDVAVVLDHPALAPHLVLTPRHMFRIESDGRILWL
                     GPNTVRPAAPGGP"
     gene            2900..4306
                     /locus_tag="VFD06_07700"
     CDS             2900..4306
                     /locus_tag="VFD06_07700"
                     /inference="COORDINATES: protein motif:HMM:NF020826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN03460.1"
                     /translation="MAASLSRDLKGLVKGEVLDDAAAREARSGDFGRMVKRVPGVVVR
                     PAGTDDVAAVLRYARKHSVPVATRGEAHTQTGQALTDGGIALDLTSLHAIHGVDTGTP
                     GGAGPSADCGAGVKWETLVRQVVPRGLVPPVLTNNLGVTIGGTLSVAGLGVASFRHGA
                     QGDNVLEIEAVTGAGDVVRCSPSQNQEVWDAVRSGLGQFGVITRARLKLRSCLTKTRT
                     YYLLYDDLAAIMRDMQMVIDQDRVDYLESWCVPCPQGFRWTGPTKEAFAAWFFPLHLT
                     VEFDPASPPDDAARLQGLQPYRKMHVEDQDLFYFAARLEPLFAVWNRSGYWANTHPWM
                     ETILPWPVSLPYITQVLNALPPTALGGGHVLLWPCRGTTSSVPLFMTPKTPLVMGFGI
                     LPGLPPDILPLVKPRLNAASDASMQAGAKRYLSGLIEFDRPRWKQHFGPKWDDVCRLK
                     KTYDPDGILNPGFIDYGP"
     gene            4358..5260
                     /locus_tag="VFD06_07705"
     CDS             4358..5260
                     /locus_tag="VFD06_07705"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN03461.1"
                     /translation="MKRALRITAHVARLALGAIFVAAGLFKSLDVAEFARQTAEYGVV
                     GPGAAGVLAPLLIALETTLGVALLAGWRPRLAAALTGGLLVVFISLEAWGISQGRTEA
                     CGCFGAYVKRTPQQVIYEDLAFLTLALIVMFGLGAWQASRRALASALTAAAAVVMLGF
                     AVASPRLPIDALVTSLKTGKSAADLGIASRLPEEGPGGTLVAILDVTDPISREAAASL
                     NTLKEGSGEVAVIALTPSSEQEQAAFMWEAYPAFEVVPMDRGLLKPLYRRLPVYFLVR
                     AGKVERIFPGPRPPGADLLSSGAS"
     gene            5257..6087
                     /locus_tag="VFD06_07710"
     CDS             5257..6087
                     /locus_tag="VFD06_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN03462.1"
                     /translation="MKTPSTWLRSVALAAICAAPIAALSNPALADTITCRAVYDFCVE
                     VDGKYPQDARFFTAPDSRGKHMIDIPSQSVSYLIDMPARKAVSIPRANVRPDGDGVVK
                     VDTSVASNAASYALSIEGPVLRFQTGNAKVRILKVLDRPPVIGEVGMDALIADRIEYR
                     EGIKRYTPDKVAMDAIKAWKKPMEIEAYFATWCAHCKMYMPKVLRVLKDAGNPNIKVA
                     LVGVPKNFGMEKGPWEGKGLQGIPVIIVKSEGREVTRLGTHEGAVPEVELAGIMSALK
                     "
     gene            complement(6088..6447)
                     /locus_tag="VFD06_07715"
     CDS             complement(6088..6447)
                     /locus_tag="VFD06_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03463.1"
                     /translation="MSDAPKSAWEIALQKLEKQDRERGTASPDPLSDDQKKQIASARQ
                     RSQAKLAEAEILHHDQRRKAREDPEALEKVEEEYRIERRRIEDAREREIDRIRSGKPK
                     PAADDARTPAGKPKKRR"
     gene            6553..6846
                     /locus_tag="VFD06_07720"
     CDS             6553..6846
                     /locus_tag="VFD06_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03464.1"
                     /translation="MSDTLNIVCPCCDTRLVIDATTGAILREDRVVKREHGSLDEAFG
                     SVRAKKREADERLEKAFSDAKHREEILEKKFQEARKKAAESPDQPPPRPFDGD"
     gene            complement(6859..7965)
                     /locus_tag="VFD06_07725"
     CDS             complement(6859..7965)
                     /locus_tag="VFD06_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03465.1"
                     /translation="MEAQDLLISTPPPRRRGRLVLPACLGAALLAVVAVRPASAQGFG
                     GTVEDILTRADNLLRQQKPNEAIAQFQEARTLCTTPFETVSALQGEARGRMQNREFLP
                     AAGLFEEAVQRFPDDPRQPDLLYMAGMARSQGGDVEGAAKLLRKALDSNPTADLVPAL
                     RFWLARSLRLSGKPKEVVDLLKDFEKEFPANQLIPRSLYYLGLAQHDSGDLAGSETTY
                     RHLIETYPHTQDTAEAFFDLGQVLAELGKREEAVQYLRTYANANPSSAVAARSMELAG
                     DLSLFHSPKEAALYYGVARVKSTGNPVPATADLQVSRWLGSKTTIARWLSEVWVLIAL
                     AVVAAAILALVGWIVLRRLKGSRSRRPAAEPSNA"
     gene            8198..9487
                     /locus_tag="VFD06_07730"
     CDS             8198..9487
                     /locus_tag="VFD06_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HZN03466.1"
                     /translation="MPAPGRPGPAELRRRVLHGPDLRGFLSEAEIWSGILERAQPGRD
                     PRRLFDAVARTEHMTTLVPTGRLDRGFPARSRRLARARLRALCPEMSALLLGLRLAHP
                     GLREGEHHFRLEARADPEQGRPADRIDLGAFGGAAGPLTIRLRHPGATLRAVLTPRSL
                     LLRQPGLPATAFPLGALELGGLLPAGPLPRPARRALIPGTSIVLAPAVRSTGRSLGAA
                     LVVGRDMPGVADRLARGLRLVSLAWPEGAREIVRRTALVVPVFERRLVSYSLAARPGV
                     SFINVRGKRTVDLADDLLHETAHHLLHEAQEAVDFLVRGPETEETQAFDSPWRRARRP
                     LHGILHGAFTFLFRAELFTRVARARRHLPGVVGPLLGRGGARFVAREGRRELSMMARA
                     LQDLGTARRAGLLTPAGRILVAGLAAWRRRLITGGSR"
     gene            9484..>11146
                     /locus_tag="VFD06_07735"
     CDS             9484..>11146
                     /locus_tag="VFD06_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HZN03467.1"
                     /translation="MTTSRRAARVLSAAAVLALALLSAAPAARPTVEEARRFVDQANE
                     RLLELNNRLQRAQWVAATYITDDTEKISSEYNEQSIAATTALALEARRFDGMTLPPDV
                     ARSLKLLKLALTLPAPNNPKERAELTEIAAWLEGTYGKGKWCPQGPDKDCLDINGVSK
                     ILAESRDPKELLDAWKGWHAVGKPMRPKYQRFVTLSNQGAKELGFADTGAMWRSNYDM
                     EQAQFAAEVDRLWNQVRPLYESLHAYVRWKLSEKYGPAVVPPTGPMPAHVLGNLWAQT
                     WEYTYPLVAPPQADPGYNVTAILQARQADPQGMVRYGEGFFSSLGLEPMPPTFWVRSM
                     FVKPPDHDVVCHASAWDVDNDQDLRIKMCIEINDEDFKTIHHELGHNYYQRAYRQQPF
                     LFRDSANDGFHEALGDTVALSITPSYLKQLGFIDKDPAPGSDTGLLLRMALDKVAFLP
                     FGLVIDQWRWKVFSGEVKPSDYNAAWWELRRKYQGVAEPLPRSEADFDPGAKYHVPAN
                     VPYTRYFLADILQFQFHRGLCRIAGQKGPLHRCSIYGNKAAGEALA"
BASE COUNT         1570 a         3998 c         3912 g         1666 t
ORIGIN      
        1 cgaacacctt gcggtaggcc tcgtccgtga ggatctcctt cagcgtcacc tcggccgccg
       61 ccagggtgac cgggttgctg gcgtacgtgc cgacgtggat cacccggagc gagttcagct
      121 cttccatgat gtccgccgag gcgccgaagg cggccagcgg catccccccg ccgatcgcct
      181 tggccaggac cgtgatgtcc ggctggacct tgtagtgctc ggtggcgccg ccgcgcgcga
      241 tcttcacgcc ggtcttcacc tcgtcgaaaa tgaggagcgc gccgtaccgg tcgcacacct
      301 cgcgcagccc cggcaggaac gccggctccg gctcggtcac ccccatgttg agcatgatcg
      361 gctcgacgat gacgcaggcg acccggttga ggttctcctc gaagcgccgc cgcaccgagt
      421 cgaggtcgtt gaactgcgcg acgtgcgtgt gctgcagcgt cccgggggga atcccctcgc
      481 tcgccggcac cgaggcgggg gcctcgcgca ggccgacgcg cccgagcggc ggcttggtgg
      541 agacgagcac ggtgtcgtgc gcgccgtggt acgccccttc gaacttgacg acgcccggac
      601 gcccggtggc gccgcgcgcc aggcggatgg cgtgcatggt cgcctcggtc ccggagttgg
      661 cgaagcgcca ctggtcctgt ccgaagcggg cgcgcagctc ctccgcgacg accgcctcga
      721 ggcggtgcgg catggcgtgc agggtcccgc gcttcacctg ccgctccacg gcggcgacga
      781 tcttgggatg ggcgtgcccg accatcatcg cgccgaacgc catcccgaag tcgatgtagc
      841 ggttgccgtc gagatcggtg atcgtcgtgc cgacggccgt ctcgacgaag atcgggttcg
      901 ggtcgaccgc ccggaaattc gacgagacgc cgcgcggcag gagcgacttg gcgcggtcgt
      961 gcgcctcgcg cgacttgcgc gtgcgcgaaa ggtactgggc ggtgatggcg tcgaggttcg
     1021 acatggcggt ctccgtggcg cgtcgggggc gtgtgagaga cggatcttag tcgatcagcg
     1081 ccccgcgtcc ggcgctccgc cccccccgtc gcgcgggatg aaacgcacct cggagtactc
     1141 cgccaccgcc tcgagccggc cgaccgcggc cccttcgtcc gacgcgtagg tttcgcgccg
     1201 ggtcgccacc cgcaggccgt cgaacggctt ctggtccgac cagaccgaca gccggtactg
     1261 gttcgacgcc gagcggtagc tgaggatctc ctccacgaga tggctgcgac cgctgatgta
     1321 cacgtcgtgc cagtcgtcgg tgctcccccc ctccatcggc cgctcgaagc gcacgcgtac
     1381 cacgtcgcag gggcccgccg ggaccttccc caccgcgggg ggcgtgcgca cgccgcgcga
     1441 ctcgagcagg atcccgggct gcagcagggt ccacggcgtc aggaactccc agcgcacctg
     1501 gcggccgaag gcgcgctcga agtccgcctg atccggatcg tccacggcgg cgccggcgcg
     1561 cgacaacgcc acctggtcgc catcgagacg cacctcgagc ggttggggcc cgtcgaggtc
     1621 ctggagcttc acgtgaactt cgtcgtcgag ggacagccag tggcgctgcg agcgggtcag
     1681 gcgcggggtc ggatcgcccc cgtagaaccg gaaacggtac tcgtactcca cttcgcgcat
     1741 cccctgccac acctcgtacc cgccgctgga gctgaggagg gcggcgaccg ccttccgcgc
     1801 cccttcgtcg cgcgtcgcca ggtgcacgcg cgcgacgatc gggtcctgct catgaggtgc
     1861 gtcgcaccca ccgaggaagg ccacggcgac cgacgcggcg gaaagcgccc gagcgaggcg
     1921 cctcacggtc cccccggcgc cgcggggcgg acggtgttcg gaccgagcca caggatgcgg
     1981 ccgtccgatt cgatccggaa catgtgccgc ggggtcagga ccaggtgggg tgccagcgcg
     2041 gggtggtcga gaaccacggc gacgtcggtc tccgtcggca gatccccctg cgcgtgcgag
     2101 tggagcgtcg gcgacgcgcc gggccgctcc ggcaccgcga cgagggaggt cgcgtcgtgc
     2161 agcggcagca tctcccccac ctgcatcccg tccccgctga gacgcagctt gaggtcggac
     2221 ccgaagcgcg ctgcgtcggt ccggtcgggc cgggtctgga ggtagacgac gtaagccacc
     2281 gcccccttct ccctgaagac cgcctcgtcg tatggcgccg gcggccctcc cgcccgggcg
     2341 gccgcagcgc tgcgcgccgc cgccaaagcg cgcaggtggc cgatgacgtc ggcaggcggg
     2401 gacttcgact ggtcgaggag gacgagtgag gcgacctcgc cggcgcgcgg ctgccaggtg
     2461 gcgtccgcga ccgcgaccaa gccgcgggtg tcctttccga cctcctcgcg tcgcagcaag
     2521 aagacgtgca tcacgccgtt gcgctccacc atcacgccgg gcgcctctcc caggtcgggc
     2581 tggcgctgcg ccagggcggc gcggcagcgc gccaccgcct cgtggtaggc ggcgatgcgc
     2641 ttcccgccgt cggtgatcgc ctgcaactcc gcgtccgtcg gccgcgccgc ggccggcggg
     2701 gcaccggccg cctgggcccg gacgagggac gcgggtgcgg cgagcagccc cacgcacgct
     2761 gcggcggcca tgacgaaact cgactgtctc atcggcccgg agtctacccc gttcttgaac
     2821 ccctcccatc gatcccgtat catgcgcggc cttccacaca ttgggccagg ggccccggac
     2881 gagggagacg gagtagcgga tggcggcgag cctgtcgcgg gacttgaaag gtctggtcaa
     2941 gggggaggtt ctcgacgacg cggcggcgcg cgaggcgcgc agcggcgact tcggccggat
     3001 ggtcaagcgg gttcccggtg tggtcgtccg cccggccggg acggacgacg tcgccgcggt
     3061 cctccgatac gcccgcaagc actcggtgcc ggtggcgacg cgcggcgagg cgcacaccca
     3121 gacggggcag gcactcaccg acggcggcat cgcgctcgac ctgacttcgc tccacgccat
     3181 ccacggcgtc gacaccggga ccccgggcgg ggccggcccg agcgccgact gcggcgcggg
     3241 ggtgaagtgg gagacgctgg tgcgccaggt ggtgccgcgc ggtctcgtcc cgccggtctt
     3301 gaccaacaac ctcggcgtca ccatcggcgg cacgctgtcg gtcgcggggc tcggggtcgc
     3361 gtcattccgc cacggcgccc agggggacaa cgtcctcgag atcgaagcgg tcaccggcgc
     3421 gggggacgtc gtccggtgca gcccgtcgca gaaccaggag gtgtgggacg cggtccgatc
     3481 cgggctcggc cagttcggcg tgatcacgcg ggcgcggctg aagctccggt cctgcctgac
     3541 gaagacccgc acctactacc tgctctacga cgatctcgcc gcgatcatgc gcgacatgca
     3601 gatggtgatc gatcaggacc gggtcgacta cctggagtcc tggtgcgtcc cctgcccgca
     3661 ggggttccgc tggaccggtc cgacgaagga ggcgttcgcc gcctggttct tcccgctcca
     3721 tctcaccgtt gaattcgacc cggcctcacc gccggacgac gccgcccgcc tgcaggggct
     3781 gcagccgtac cggaagatgc acgtcgagga ccaggatctc ttctatttcg cggcgcggct
     3841 cgagccgctc ttcgcggtct ggaaccgcag cggctactgg gccaacaccc atccctggat
     3901 ggagacgatc ctgccgtggc cggtctccct cccgtacatc acgcaggtgc tgaacgccct
     3961 tccgccgacc gccctcggcg gcgggcatgt gctcctgtgg ccgtgccgcg gcaccacctc
     4021 gagcgtgccg ctcttcatga cgccgaagac gccgctggtg atgggattcg ggatcctgcc
     4081 ggggctgccg ccggacatcc tgccgctcgt gaagccgcgc ctcaacgccg cctccgatgc
     4141 cagcatgcag gcgggagcaa aacgctatct ctccggtctc atagagtttg acaggccgcg
     4201 ctggaagcag cacttcggcc cgaagtggga cgatgtctgc cggctcaaga agacgtacga
     4261 cccggacggc atcctcaatc cggggttcat cgactacggc ccctgaggct tcaagaagcc
     4321 tagccccgga aggtcaccgg ggcgcggcga gttcgggatg aaacgagcgc ttcggatcac
     4381 cgcccacgtg gcgcgcctcg ctctcggggc catcttcgtc gccgccggtc tcttcaaatc
     4441 gctcgacgtc gcggagttcg cccgccagac ggcggagtac ggcgtcgtcg gccccggcgc
     4501 cgcgggggtc ctcgctccgc tgctcatcgc cctcgagacg accctcggtg tcgcgcttct
     4561 cgccggctgg cgtccgcgcc tggcggcggc gctgaccggc ggcctgctcg tcgtcttcat
     4621 ctccctcgag gcctggggca tcagccaggg gcgcacggag gcctgcggct gcttcggcgc
     4681 gtacgtgaag cgcacgcccc agcaggtgat ctacgaggac ctcgccttcc tcacgctggc
     4741 gctcatcgtg atgttcgggc tcggggcctg gcaggcgtcg cgccgggcgc tcgcctcggc
     4801 cctgactgcc gccgccgcgg tcgtcatgct cgggttcgcc gtggcgtcgc ctcgactgcc
     4861 gatcgacgcg ctcgtgacct cgctcaagac cgggaaaagc gccgccgatc tggggatcgc
     4921 gtcgcgactt cccgaggagg ggccgggagg gaccctggtc gccatcctcg acgtcaccga
     4981 cccgatctcc agggaggcgg ccgcctcgct caacaccctc aaggaaggga gcggagaggt
     5041 ggcggtcatc gccctgaccc cctcgagcga gcaggagcag gcggctttca tgtgggaggc
     5101 gtaccccgcc ttcgaggtcg tgccgatgga tcgggggctc ctgaagccgc tgtaccggag
     5161 gcttccggtg tacttcctgg tccgggccgg caaggtggag cgaatcttcc ccggccctcg
     5221 cccgcccggg gccgacctgt tatcatcggg ggcctcatga agaccccctc gacatggctt
     5281 cgtagcgtgg ccctggcggc gatctgcgcc gctcccatag ccgccctctc caacccggcg
     5341 ctggccgaca cgatcacctg ccgcgccgtc tacgacttct gtgtcgaggt ggacgggaag
     5401 tacccgcagg acgcgcgctt cttcaccgcc cccgacagcc gcggcaagca catgatcgac
     5461 atcccgtccc agtcggtgag ctacctcatc gacatgccgg cccgcaaggc ggtgtcgatc
     5521 ccccgcgcca acgtccgtcc ggacggtgac ggtgtggtga aagtggacac gagcgttgct
     5581 tcgaacgccg cctcctatgc cctgtcgatc gaagggccgg tcctccgctt ccagacgggc
     5641 aacgcgaagg tgcggatcct gaaggtgctc gaccgcccgc cggtgatcgg tgaggtcggc
     5701 atggacgccc tgatcgccga tcgcatcgag taccgcgagg ggatcaagcg ctacaccccc
     5761 gacaaggtcg cgatggacgc gatcaaggcc tggaagaagc cgatggagat cgaggcctac
     5821 ttcgcgacct ggtgcgccca ctgcaagatg tacatgccga aggtgctgcg cgtcctcaag
     5881 gacgccggca acccgaacat caaggtcgcg ctcgtcggcg tcccgaagaa cttcggcatg
     5941 gagaagggcc cctgggaagg gaaagggctc cagggaatcc cggtcatcat cgtgaagtcc
     6001 gaagggcgcg aagtgacccg cctcggaacg cacgaggggg cggtgccgga ggtcgagctc
     6061 gccggcatca tgagcgccct gaagtagtca gcgtcgcttc ttcggcttcc ccgccggtgt
     6121 gcgcgcgtcg tccgcggccg gcttcggctt tccggaccgg atccgatcga tctcccgctc
     6181 gcgcgcatcc tcgatgcggc gccgctcgat gcggtactcc tcctccacct tttcgagcgc
     6241 ctccgggtcc tcccgcgcct tgcggcgctg gtcgtggtgc aggatctccg cctccgccag
     6301 cttcgcctgg gagcgctgcc gcgcggacgc gatctgcttt ttctgatcgt cgctcagggg
     6361 gtcggggctc gcggtcccac gctcccggtc ctgcttttcg agcttctgga gcgcgatctc
     6421 ccaggcgctc ttcggggcat cgctcatgcg cgccggctcc tcgtcatagg tgtcaggaat
     6481 gcccgccgct tccgctcctt gaccgcggcc gacgggcaga cggagaatag cgctaaatcc
     6541 ggcgaggagc ccatgtccga tacgctcaac atcgtctgcc cgtgctgcga cacgcggctc
     6601 gtcatcgacg ccacgacggg cgccatcctg cgcgaggacc gcgtcgtcaa gcgggagcac
     6661 ggctccctcg atgaggcgtt cggctcggtg cgggcgaaga agcgcgaggc ggacgagcgg
     6721 ctggagaagg ccttctcgga cgcgaagcac cgcgaggaga tcctcgagaa gaagttccag
     6781 gaggcccgca agaaggccgc ggagtctccc gaccagcccc cgccccgtcc gttcgacggc
     6841 gactgaacct ccttcaagtc aggcgttcga cggctcggcg gcggggcggc gcgagcgtga
     6901 ccccttcagg cgcctgagga cgatccagcc gacgagcgcc aggatcgcgg ccgcgacgac
     6961 cgccagcgcg atcaggaccc acacctccga gagccagcgg gcgatcgtcg tcttggatcc
     7021 gagccagcgg ctgacttgca ggtcggcggt ggcggggacc gggttgcccg tcgacttcac
     7081 cctggccaca ccgtagtaca gcgccgcctc cttcggggag tggaaaaggc tcaggtcccc
     7141 ggcgagctcc atggaccggg ccgccacggc ggacgacggg ttggcgttgg cgtaggtgcg
     7201 caggtactgg accgcctcct cccgcttgcc gagctccgcc agaacctgtc cgaggtcgaa
     7261 gaaggcctcc gccgtgtcct gggtgtgcgg gtaggtctcg atcaggtgcc ggtaggtcgt
     7321 ctccgatccg gccagatcgc cggagtcgtg ctgcgccagg ccgaggtagt agagcgaccg
     7381 cgggatgagc tggttcgccg ggaactcctt ctcgaaatcc ttgaggagat cgacgacctc
     7441 cttcggcttg ccgctgagcc tcaggctcct ggccagccag aagcggaggg ccggcacgag
     7501 gtcggcggtg gggttgctgt cgagcgcttt ccggaggagc ttcgcggccc cctcgacatc
     7561 acctccctgg ctgcgcgcca tgcccgccat gtagaggagg tccggctgcc gggggtcgtc
     7621 cgggaaccgc tgcaccgctt cttcgaaaag cccggcggcc ggcaggaact cgcggttctg
     7681 catgcgcccc cgcgcctccc cctgcagagc gctgaccgtt tcgaagggtg tggtgcagag
     7741 ggtgcgcgcc tcctggaact gggcgatcgc ctcgttcggc ttctgctgcc gcaggaggtt
     7801 gtccgcccgg gtcaggatgt cttcgacggt cccgccgaac ccctgggcgg aggcgggccg
     7861 gaccgcgacg accgcgagca gcgccgcacc gagacaggcg gggagcacca ggcggccccg
     7921 gcggcggggc ggtggcgtgc tgatgagcag gtcttgggcc tccatcgcgg cgcgtattct
     7981 atgatccctc cgccgggacg tcatccctgc gccgcatgcc cgcccccatc tggagtctga
     8041 ttcaccacct cgcccctccg ggcgaggagg cgagcctccg gctcgcgcga ctggcggagg
     8101 ggcgcaccgc gcggcggctc gcgcggctgg aggggtgctt gcagcgggcg tcgcttcccc
     8161 cctccgagcg ggagcccctg cgccggctcg tcgccgaatg ccggcgcctg gtcgcccggg
     8221 ccccgcagag ctgcggcggc gcgtcctgca cggccccgat ctgcgcggct tcctctccga
     8281 ggcggagatc tggagcggca tcctcgagcg ggcgcagccc gggcgcgatc cacggcggct
     8341 gttcgatgcg gtggcccgga ccgagcacat gacgacgctc gtcccgacgg ggcggctcga
     8401 tcgggggttc ccggcccgct cgcggcgtct cgcgcgggcg cggctgcgcg cgctttgccc
     8461 ggagatgtcc gccctgctcc tgggcctccg tctggcgcat cccgggcttc gcgaggggga
     8521 gcaccacttc aggctcgagg cgcgcgccga tcccgagcag gggcggccgg ccgaccggat
     8581 cgacctcggc gcgttcggcg gcgccgcggg gccgctgacc atccggctgc gacaccccgg
     8641 cgcgacgctg cgggccgttc tgacgccgcg ctcgctcctg ctccggcagc cgggcctgcc
     8701 cgcgacggcg tttccgctgg gggcgctgga actcggaggg ctgctgccgg caggtcccct
     8761 gccgcgcccg gcgcgccgcg cgctcatccc cggcacctcg atcgtcctcg cgccggcggt
     8821 gcggtcgacc gggaggtcgc tcggcgccgc gctcgtcgtg ggccgcgaca tgcccggggt
     8881 ggcggaccgg ctggcgcgcg ggttgcggct cgtgtccctc gcctggccgg aaggggcccg
     8941 cgagatcgtc cggcgtacgg cgctggtggt gccggtcttc gagcggcgcc tcgtgtcgta
     9001 ctcgctggcg gcgcggcccg gggtgtcgtt catcaacgtc cgggggaaac ggacggtcga
     9061 tctcgccgac gacctcctgc acgagacagc gcaccacctg ctgcacgaag cgcaggaggc
     9121 ggtcgatttc ctggtgcgcg gcccggagac cgaggagacc caggcgttcg actccccgtg
     9181 gcgccgcgcc cgccggccgc tccacgggat cctgcacggc gccttcacgt ttctgttccg
     9241 cgccgagctg ttcacgcgcg tcgcgcgggc gcggcgacac ctccccgggg tcgtcggccc
     9301 gctcctcggg cgcggcgggg cgcgcttcgt cgcccgagag ggccggcgcg agttgagtat
     9361 gatggcgcgc gctctgcaag acctgggaac cgcccgcagg gccggactcc tgacgccggc
     9421 tgggcggata ctcgtcgccg ggctcgcggc ctggcgccgc cggctgatca ctggaggatc
     9481 gagatgacga cgtcccgtcg cgccgcccgc gtcctgagcg ccgccgccgt tcttgccctc
     9541 gccctgctct cggccgcacc cgctgcaaga ccgaccgtcg aggaggcgcg ccgcttcgtg
     9601 gaccaggcga acgagcggct gctcgagctc aacaaccggc tgcagcgggc gcagtgggtc
     9661 gccgccacct acatcaccga cgacaccgag aagatctcct ccgagtacaa cgagcagtcg
     9721 atcgcggcca cgacggcgct ggcgctcgag gcgcgccggt tcgacgggat gacgctcccc
     9781 cccgacgtgg cgcgctccct caagctcctg aagctggcgc tcaccctccc cgcgccgaac
     9841 aacccgaaag agcgggccga gctgaccgag atcgccgcgt ggctcgaagg gacctacggc
     9901 aaggggaaat ggtgcccgca gggccccgac aaggactgcc tcgacatcaa cggcgtctcg
     9961 aagatcctcg ccgagagccg cgaccccaag gagttgctcg acgcctggaa gggctggcat
    10021 gccgtcggaa agccgatgcg cccgaagtac cagcgcttcg tcacgctctc gaaccagggg
    10081 gcgaaggagc tcgggttcgc cgacaccggg gcgatgtggc gctcgaacta cgacatggag
    10141 caggcgcagt tcgcggcgga ggtggaccgc ctctggaacc aggtgcggcc gctgtacgag
    10201 tcgctgcacg cctacgtccg ctggaagctg tcggagaagt acggccccgc cgtcgtccct
    10261 ccgaccggcc ccatgccggc gcacgtcctc gggaacctgt gggcgcagac ctgggagtac
    10321 acctacccgc tcgtggcgcc gccacaggcc gatcccggct acaacgtcac ggcgatcctg
    10381 caggcgcgcc aggccgaccc gcaggggatg gtgcgctacg gtgaagggtt cttctcgtcg
    10441 ctcgggctcg agccgatgcc cccgacgttc tgggtgcgct cgatgttcgt gaagcccccc
    10501 gaccacgacg tcgtctgcca cgccagcgcc tgggacgtcg acaacgatca ggacctgcgc
    10561 atcaagatgt gcatcgagat caacgacgag gacttcaaga cgatccacca cgagctgggg
    10621 cacaactact accagcgcgc ctaccggcag cagccgttcc tcttccgcga cagcgccaac
    10681 gacggcttcc acgaggcgct cggcgacacg gtggcgctgt cgatcacgcc gtcgtatctg
    10741 aagcagctcg ggttcatcga caaggacccg gcgccgggga gcgacaccgg gctgctgctc
    10801 aggatggcgc tcgacaaggt ggcgttcctg ccgttcggcc tggtgatcga ccagtggcgc
    10861 tggaaggttt tctcgggcga ggtgaagccg tccgactaca acgccgcctg gtgggaactg
    10921 cggcggaagt accagggcgt ggcggagccg ctgccccgca gcgaggccga cttcgatccc
    10981 ggcgccaagt accacgtgcc ggcgaacgtg ccctacaccc gctacttcct ggcggacatc
    11041 ctccagttcc agttccaccg cgggctctgc cgcatcgccg ggcagaaggg tccgctccac
    11101 cgctgctcga tctacgggaa caaggcggca ggagaggcgc tcgcca
//
LOCUS       DASRIQ010000321         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3248632, whole genome shotgun sequence.
ACCESSION   DASRIQ010000321 DASRIQ010000000
VERSION     DASRIQ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248632"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2269)
                     /locus_tag="VFD06_07740"
     CDS             complement(<1..2269)
                     /locus_tag="VFD06_07740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN03468.1"
                     /translation="MSETLQPKRLTIVLALAAILAAALALRLSYVFQVVGSSLVTPTD
                     LDPGFYYNWAKRIAAGDWLGRDPFVQSPLYAYLLGAFMKFFGTGLTPILVAQSVVGVG
                     TVFLTYVAGRRFFSPTHGLIAAALMAVYGPFVFFEGMVMKTFLSPFLTLVLALALERA
                     ADGAARGAAAMGRFAVAGAVFGILTLDRDNFILLAPALAALAYLLGRGPKRDGLTPEG
                     FTGGADAFARRSGLKAAAAFTLGTVLLIAPATFRNWVVAKEFVLLTTGGGEVFFIGNN
                     ADANGLYLPPPFVRPDPLYEHTDFVERGSEIAGRPLTPMQSSWFWFKEGTKFIAGEPL
                     AWLRLEAKKLFFFWNWYELPDNLDYGVMQYFSPILRRLNVAVPPAGLPAPALPIAPGA
                     WAPSRLHLYGTFGTVAPLGLLGLVLSLRRRRVSLKTLYVLLFGYMATVLLFFNFARFR
                     VPVVPILAIFAAEGVLVTWAFLRRLGTFLGGLARRSGDLAERARALVTGPEGVAAGAF
                     GVAAILLVNLEMPRGVVPAIEQALLIGNAYYAQGKSRLARDSYTGGLVLLGEGPPGER
                     GDALLRDRFGPGVTVEALKRELDVESVARGPQFKGIHLGIHHGLGIAMVQEAQALLDE
                     GRRVEAMALLDQAVAQFDEALRIAPSYMLSHRKMARAWLLKGDSPRAIEWLQKGIDLW
                     PDDVPARLELAELLYSTGDYRGAMRQLDAARHYNASMEPATLAQIHLDRGLVLLRGLD
                     DPGRALWNLEQSLALA"
     gene            complement(2365..2541)
                     /locus_tag="VFD06_07745"
     CDS             complement(2365..2541)
                     /locus_tag="VFD06_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03469.1"
                     /translation="MPLAARTCGVTDAWVLDGVLLEPPEAVFYLVTGNGPGGETGLGT
                     NSAGQPRPNANPCP"
BASE COUNT          462 a          970 c          975 g          351 t
ORIGIN      
        1 gcgccagggc cagcgactgc tcgaggttcc acagcgcccg gcccggatcg tcgagaccgc
       61 gcaggagcac gagcccgcga tcgaggtgga tctgcgccag cgtcgccggc tccatcgagg
      121 cgttgtagtg ccgggcggcg tcgagctgcc gcatggcccc gcgatagtcg ccggtcgagt
      181 agagcagctc ggccagctcg agccgcgcgg gcacgtcgtc cggccagagg tcgatcccct
      241 tctgcagcca ctcgatggcg cgtggcgagt cccccttgag gagccaggcg cgggccatct
      301 tgcggtgcga cagcatgtac gagggggcga tgcgcagcgc ttcgtcgaac tgcgcgaccg
      361 cctgatcgag gagcgccatc gcctccaccc gccgcccctc gtcgaggagc gcctgcgcct
      421 cctgcaccat cgcgatgccc agaccgtggt ggatgccgag atggatccct ttgaactgcg
      481 ggccgcgcgc caccgactcg acgtcgagct cgcgcttcag cgcctccacc gtcactcccg
      541 ggccgaagcg gtcgcgcagg agcgcgtccc cccgttcgcc cgggggaccc tcgccgagca
      601 gcacgagccc gccggtgtag ctgtcgcgcg ccaggcgcga cttcccctga gcgtagtagg
      661 cgttgccgat caggagcgcc tgctcgatcg ccggaaccac gccgcgcggc atctcgaggt
      721 tgaccagcag gatcgcggcg acgccgaagg ccccggccgc gaccccttca ggaccggtga
      781 cgagggcccg ggcgcgttcg gcgagatcgc cggagcggcg agccagcccc ccgaggaatg
      841 tcccgagccg gcgcaggaac gcccaggtca ccagaacacc ctcggccgcg aagatcgcca
      901 ggatcggcac gaccggcacg cggaagcggg cgaagttgaa gaacagcagg acggtcgcca
      961 tgtagccgaa gaggagcacg tacagtgtct tcagcgagac ccggcggcgc cgcagcgaca
     1021 ggacgagccc gaggagtccg agcggcgcca ccgtcccgaa cgtcccgtag aggtgcaggc
     1081 gcgacggcgc ccaggccccc ggggcgatcg gcagcgccgg cgccggcagc cccgccggcg
     1141 ggacggccac gttgagccgc cgcaggatcg gcgagaaata ctgcatgacg ccgtagtcga
     1201 ggttgtccgg cagctcgtac cagttccaga agaagaacag cttcttcgcc tcgagccgga
     1261 gccacgccag aggctcgccc gcgatgaact tcgtcccctc cttgaaccag aaccaggagg
     1321 actgcatcgg cgtcagcggc cgcccggcga tctcgctgcc gcgctcgacg aaatcggtgt
     1381 gctcgtagag cggatcggga cgcacgaacg gcggaggcag gtacagcccg ttcgcgtcgg
     1441 cgttgttgcc gatgaagaag acctcgccgc cgccggtcgt caacaggacg aattccttcg
     1501 cgacgaccca gttgcggaaa gtcgccgggg cgatcagcag caccgtaccg agcgtgaacg
     1561 cggcggccgc cttgagcccg gaccggcgcg caaaggcgtc ggcgcctccc gtaaaaccct
     1621 ccggcgtgag gccgtcccgc ttcgggccgc gccccagcag gtacgccagc gccgcgagcg
     1681 ccggggcgag caggatgaag ttgtcgcggt cgagagtcag gattccgaag accgcgccgg
     1741 ccacggcgaa ccgtcccatc gccgccgcgc cgcgcgccgc cccgtccgcc gcccgctcca
     1801 gcgccagcgc cagcacgagc gtcaggaagg gggagaggaa ggtcttcatg accatcccct
     1861 cgaagaagac gaaggggccg tagacggcca tcagggcggc cgcgatgagc ccgtgcgtcg
     1921 ggctgaagaa ccgccggccg gcgacgtacg tgaggaacac cgtgccgacg ccgaccaccg
     1981 actgggcgac gaggatcggc gtcagccccg tgccgaagaa cttcatgaag gcgccgagca
     2041 ggtaggcgta gagggggctc tgcacgaacg ggtcgcgccc cagccagtcc ccggcggcga
     2101 ttctcttcgc ccagttgtaa tagaagccgg ggtcgaggtc ggtcggcgtc acgagcgatg
     2161 agccgaccac ctggaagacg tacgacagcc gcagcgccag cgccgcggcg aggatcgccg
     2221 cgagcgccag aacgatcgta agtctcttcg gttgtagggt ttccgacacc tggactccgc
     2281 ccccgaatgg ggcggcggat tatatcaggc ctgtccgcgc cggcctcgac gtccggcgaa
     2341 cgggaggaac gcagacccgg ccggctaggg gcagggattc gcgttcggcc tcggctgtcc
     2401 ggcgctgttc gtccccaggc cggtctcgcc gccggggccg tttccggtca cgaggtagaa
     2461 gaccgcctcc ggcggctcga gcaggacccc gtcgaggacc caggcgtccg tcacgccaca
     2521 ggtgcgcgcc gccaagggca ccgtggcagg gttctgcgtg tagatgccgg tctgtcgcag
     2581 gaccgccagg ttcccgcgat agacgttgaa cgacgggaag ccctgctccc gttggcaatc
     2641 gacatgacac ggccgcctat ccctgctctt cgcgcatctg gtcgaggcgg gcgaggaagt
     2701 cgggctcgac gaggacctcg cgcggcttgc tgccgtcggc ggggccgacg atgccgtt
//
LOCUS       DASRIQ010000322         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_907751, whole genome shotgun sequence.
ACCESSION   DASRIQ010000322 DASRIQ010000000
VERSION     DASRIQ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907751"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VFD06_07750"
     CDS             <1..1398
                     /locus_tag="VFD06_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HZN03470.1"
                     /translation="EGLKIALDHLDEMVALIRQARTPDEARQAMRLRFRLSEVQATSI
                     LEMRLQRLTGLERQKIVDEYTETLKEIARLREILANERLVYGLMVEEFREIRKAYGDP
                     RRTEIVPAPGEISVEDLIAEEEMVITVSHAGYMKRNPLTLYRSQRRGGKGRTGMTTRE
                     EDFVEHLFVASTHASILIFTTKGRVHWLKVHEIPQIGAAGKGKNIINFVNLTSEEKVA
                     AMVAVKDFTEDRTVVFCTRKGIVKKTPLAAYGNPRAGGIIAIGVDEGDELLSVNLTDG
                     KQELFMATSQGQAIRFPEGDVRPMGRGAHGVIGMRLEGEDAIVEMDCLKPDAGTMLTV
                     TEKGYAKRTEVSEYRLQGRGGSGIINLKVTDKTGPVVQVMQVAEHDQVMVITAFGKII
                     RTRVRHISLLGRPTQGVRLIHLDEGDTVVAVARVAEGDDESETGGNGRAHGDDGSPGG
                     EGAAGPPPTGPEPAR"
     gene            1395..>2187
                     /locus_tag="VFD06_07755"
     CDS             1395..>2187
                     /locus_tag="VFD06_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZN03471.1"
                     /translation="MSRDPLKELRRRDREAEAGGGEERNARQRAAGKLPARERVTLLL
                     DPGSFQELDKFVVHRSLDFGMDEQKVPGDGVITGHGAIDGRPVFVFAQDFTVFGGSLS
                     ETHAQKVCKVMDLAMKIGAPIIGLNDSGGARIQEGVVSLAGYADIFLRNTLASGVVPQ
                     ISAIMGPCAGGAVYSPAITDFNIMVEKTSYMFITGPDVVRSVTHEEVTKEELGGAVAH
                     ASVSGVAHFTATDDRAALGLIRDLMAYLPSNNMEDPPHRDTGDDPD"
BASE COUNT          384 a          736 c          776 g          291 t
ORIGIN      
        1 gaggggctca agatcgcgct cgaccacctc gacgagatgg tcgccctgat ccggcaggcg
       61 cgcacccccg acgaggcgcg ccaggcgatg cggctgcgct tccggctgtc ggaggtgcag
      121 gccacgtcga tcctggagat gcgcctgcag cgcctgaccg gcctggagcg ccagaagatc
      181 gtcgacgagt acaccgagac cctcaaggag atcgcccgcc tccgggagat cctggcgaac
      241 gagcggctgg tgtacggcct gatggtcgag gagttccggg agatcaggaa ggcgtacggt
      301 gacccgcgcc gcaccgagat cgtcccggcc cccggggaga tctcggtcga ggacctgatc
      361 gccgaggagg agatggtgat cacggtctcc cacgccggct acatgaagcg caaccccctg
      421 accctgtacc gcagccagcg ccgcggcggc aaggggagaa cgggtatgac gacgcgcgag
      481 gaggacttcg tcgagcatct gttcgtcgcc tcgacgcacg cgtcaatcct gatcttcacg
      541 acgaaggggc gggtccactg gctgaaggtc cacgagatcc cgcagatcgg cgccgccggc
      601 aaggggaaga acatcatcaa cttcgtgaac ctcacctcgg aggagaaggt ggcggcgatg
      661 gtcgccgtca aggacttcac cgaggaccgg accgtcgtct tctgcacccg caaggggatc
      721 gtcaagaaga ccccgctcgc cgcctacggc aacccgcgcg ccggcggcat catcgccatc
      781 ggcgtcgacg agggggacga gctgctctcc gtcaacctga ccgacgggaa gcaggagctg
      841 ttcatggcga cgtcccaggg gcaggcgatc cgcttcccgg agggggacgt gcggccgatg
      901 ggccgcggcg cccacggggt gatcggcatg cgcctcgagg gggaggacgc catcgtcgag
      961 atggactgcc tgaagccgga cgccggcacg atgctgacgg tcaccgagaa ggggtacgcc
     1021 aagcggaccg aggtgtccga ataccggctg caggggcgcg gcggctccgg gatcatcaac
     1081 ctcaaggtca ccgacaagac cggcccggtg gtgcaggtga tgcaggtcgc cgagcacgac
     1141 caggtgatgg tcatcaccgc cttcgggaag atcatccgta cccgcgtgcg ccacatctcg
     1201 ctcctcggcc gcccgaccca gggggtgcgc ctcatccacc tcgacgaggg ggacacggtc
     1261 gtcgcggtgg cgcgcgtcgc cgaaggggac gacgaatcgg agaccggcgg caacggtcgg
     1321 gcgcacggcg acgacggcag cccgggtgga gagggggcgg ccgggccgcc tccaaccggg
     1381 ccggaaccgg cccgttgagc cgggatcccc tcaaggagct gcgccgccgc gatcgcgaag
     1441 cggaggcggg cggcggcgag gagcgcaacg cgcgccagcg cgccgcgggc aagctccccg
     1501 cccgcgagcg ggtcaccctc ctgctcgatc ccggctcctt ccaggaactg gacaagttcg
     1561 tcgtccatcg ctccctcgac ttcgggatgg acgagcagaa ggtcccgggg gacggggtga
     1621 tcaccggcca cggcgcgatc gacggccggc cggtcttcgt cttcgcgcag gacttcaccg
     1681 tcttcggcgg atcgctctcc gagacccacg cccagaaggt ctgcaaggtg atggacctgg
     1741 cgatgaagat cggggcaccg atcatcgggc tgaacgactc cggaggggcg cgcatccagg
     1801 agggggtcgt ctccctcgcc ggctacgccg acatcttcct gcgcaacacg ctggcctcgg
     1861 gagtcgtgcc gcagatctcc gccatcatgg ggccgtgcgc cggcggcgcc gtctactccc
     1921 ccgccatcac cgacttcaac atcatggtgg agaagacgtc gtacatgttc atcaccgggc
     1981 cggacgtggt tcgctcggtg acgcacgagg aggtcacgaa ggaggagctc ggcggtgcgg
     2041 tggcccacgc cagcgtctcg ggggtcgccc acttcacggc gaccgacgac cgggccgcgc
     2101 tcgggctcat ccgggatctg atggcctact tgccgtcgaa caacatggaa gacccgccgc
     2161 accgcgacac cggggacgat ccggatc
//
LOCUS       DASRIQ010000323         8118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_381372, whole genome shotgun sequence.
ACCESSION   DASRIQ010000323 DASRIQ010000000
VERSION     DASRIQ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381372"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /gene="accD"
                     /locus_tag="VFD06_07760"
     CDS             complement(<1..952)
                     /gene="accD"
                     /locus_tag="VFD06_07760"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZN03472.1"
                     /translation="MEIAWFKKIRKPKQAVEHRRIQVPEGLWVKCNSCKEIIYKKEVL
                     RNANVCPKCGYHFRISARERLSSLFDEGRYQECDTDIYSVDPLKFRDAKPYRDRLREY
                     RDKTGLHDAVINACGSLGGSPVMMSVMEYGFMGGSMGSAVGEKVTRAIERALDEHVPL
                     IVISCSGGARMQEGALSLMQMAKISGALGKLHEARLPFVSILTDPTTGGVTASYAMLG
                     DLNIAEPKALIGFAGPRVIEQTIREKLPEGFQRSEFLLEHGMLDFIVERRQMRDTLIQ
                     CLRVLYNKEIARAGHPETGAPAGETGRSDEAVTVKEREGLG"
     gene            1515..3491
                     /locus_tag="VFD06_07765"
     CDS             1515..3491
                     /locus_tag="VFD06_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZN03473.1"
                     /translation="MTDLSGLLGPVMMAGAAAVLGYLAARRKDKPRAGRAAHARLHPS
                     GAAGAALWEDVSPEPLEATLQALIQAIDARNPAARGHVRRVRAAAMELGRGCGLDAEA
                     LATLRLAALLHDVGTLIVPEQILSKPGRLNDAEFDKVKAHPVAGVEMLAPAGLPVGVL
                     EIIRHHHERWDGGGYPDRLKGMEIPLGARILAVADAFEALVSERAHRGGLAPSEAVDL
                     ILTWAGVQFDRDVVAVLRSEVTAVHEAMAAADHLPEPPLAIARLASQSAGAPEQATSD
                     PGGFGMLLHRGISAGGAAAPSGAEAPRATAAVSRTPGDASAAQREVYALYEIARTLCT
                     SLHLTDVLDLVVGKIAMLVPFRTCVVFLTAGPRELQARFVSGANAASLRGRRLEYGDG
                     ITGWAAEHRTSSFSGTAELDLFGSEVDPAAYSTVAAFPLVQGDEVLGVITLYFPANGP
                     CHDDHVRVMEILARLAAGAIHEGRLGLEQGETGLTDAVTNLPNERYLRQAFEKEVLRS
                     QQSGQPLSLVEMDLEDFRILNERIGAEAGDRFLMEVGRILRSHLRERDVLVRLTEDGY
                     AALLPGTGFAAAALLTERLQRAVDGFVLRLETGGTARAGLSIGIAIHPVDGEGLDDLL
                     QRASINRTRNRQARRAARAETPNVVPFRPGHGGA"
     gene            3770..4777
                     /locus_tag="VFD06_07770"
     CDS             3770..4777
                     /locus_tag="VFD06_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03474.1"
                     /translation="MPRFRCASCGRSFSRQSFGTSYYLKRPELLLPIAAGLVAGSAHR
                     QMARSFGCAPSTVTRLSARLGRHTLLLHALALRHLAGRLSEPLAADHFETFEFSQDFP
                     FGVLTLVGRDSWFVYGLDPAPHARGGKVTPYQAVRLAARPRIPRRGGYRGSFRRGLDV
                     LLPLSHSTVEIACDGKEPYMLALRKHPQRDRIRLEVHPNPVRGPKGAPRSRQALARDR
                     AMYAVDQLHMLLRHSRADHKRETMAFGRRLNAILERLHLAIVWRNFIKGRSERRPDPD
                     TPGMRLGLSDCPWTWRRVLARRLFPCRASLTPVLRDLYWRNWRTPILPTNHLHRLRHA
                     A"
     gene            4860..6365
                     /locus_tag="VFD06_07775"
     CDS             4860..6365
                     /locus_tag="VFD06_07775"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZN03475.1"
                     /translation="MPAGPAAALAALTLIVNASVLDGTGAPPKNVAVRIDAGRIVEVG
                     DLKPRPGETVIDAKGLSLAPGFIDTHSHADEELFEHPDALADVSQGITTIVVGQDGGS
                     PFPLKKFFSRLERKPAAVNVASYSGHGTLRDRVMREDFRRKATAEEVRKMERLLDKDM
                     KAGALGLATGLEYDPGIYSDPSEVIALAKAAARHAGRYISHVRSEDRNFWEAIEELLT
                     IGRDARLPVQISHTKLAMKSWWGQSARLLKRLDEARASGVDVTADIYPYLYWHSTLTV
                     LFPKRNFEDMEEARLVVNEIAPPDGLILTQYDAEPTWVGKSLAEVAASRNEDPAVTLV
                     MLVRRALEHEKETGDAGEGVLGTSMTEEDFEALLRWPHMNLCTDGALEGSHPRGFGAF
                     PRVFGRYVRERHVLTLEEAVRRASGLAAENMGFRDRGLIRPGMAADLVLFDPDTIADR
                     ATITTPHALSEGVNTVWVNGVVVFQEGRATGLRPGSVLRNFSTQGDRTSLP"
     gene            complement(6281..7477)
                     /locus_tag="VFD06_07780"
     CDS             complement(6281..7477)
                     /locus_tag="VFD06_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN03476.1"
                     /translation="MSGRTTEILPRLLLILGGTIAGLLLAEVVVRVALPPPPTVVIAR
                     TDAVTRRAAETRAPKSLRVVTQGDLGPYGFVLETPAGLRMRASSEARIQNYPGATADI
                     IVRTNSLGYRNPEIGPKTKPRVLFLGDSVTLSQYLPEEASFVRRVQALSEQDGGPPFE
                     TINAAVAGIGLANEIAILEETGLSTAPDIVVIGFFLNDAEASRGVRLMQPPALLRWSW
                     LARQAGAMIPALLEPESFSGESWETGAWRAELRRLYPPGPGDPAVDRPAFNAEVLASY
                     FDWGSAWTDSAWGRIEPLLMQFKRLSAEHRFRPMLLAFPATAQVQARFDASEPQRRLG
                     AIARSLDLPLLDLLPRLRETVPKAPRPILADHCHYTDYGNEVLSPWVLKFLRTDPGLR
                     PVARPS"
     gene            complement(7508..>8118)
                     /locus_tag="VFD06_07785"
     CDS             complement(7508..>8118)
                     /locus_tag="VFD06_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03477.1"
                     /translation="TTTVVSPILDLTGRTSVVLEMALFYSNSGGNNPGEDPYRVHVSG
                     NGGASWTPVLQTTGDIASWTTTPLVLDGLIPFNNQFRVRVTAEDLGVGGSLVEAGFDD
                     VSLYQPGAGCSVCSGPVATVGTIQVNRSDDDIVLDWSADPVNASAYEVYMRSGSGLAT
                     LVRAGSTTTKSFVHTGAGLLTDQNLFYVVTAVDSCGRESAPF"
BASE COUNT         1240 a         2832 c         2816 g         1230 t
ORIGIN      
        1 ggccgagccc ttcgcgctct ttgaccgtca ccgcttcgtc ggagcggccg gtctcgccgg
       61 ccggggcgcc ggtttccggg tgcccggcgc gcgcgatctc cttgttgtac aggacgcgca
      121 ggcactggat gagcgtatcg cgcatctgcc ggcgctcgac gatgaagtcg agcatgccgt
      181 gctccagcag gaactcggag cgctggaacc cctcgggcag cttctcgcgg atcgtctgct
      241 cgatgacgcg cgggccggcg aagccgatga gcgccttcgg ctcggcgatg ttgaggtcgc
      301 cgagcatggc gtacgacgcg gtcacgccac ccgtggttgg gtcggtcagg atcgagacga
      361 acggcaggcg cgcctcgtgc agcttcccga gagcgcccga gatcttcgcc atctgcatca
      421 gcgacagcgc cccctcctgc atgcgggccc cgcccgagca ggagatgacg atcagcggca
      481 cgtgctcatc gagggcccgc tcgatcgccc gggtcacctt ctcgccgacc gccgagccca
      541 tcgagccccc catgaagccg tactccatca ccgacatcat gacgggggag ccgccgaggc
      601 tgccgcacgc gttgatcacg gcgtcgtgca gcccggtctt gtcgcggtac tcgcgcaggc
      661 ggtcgcggta cggcttggcg tcccggaact tcagggggtc caccgaatag atgtcggtgt
      721 cgcactcctg gtagcgccct tcgtcgaaca gcgacgagag ccgctcgcgc gccgagatgc
      781 ggaagtggta gccgcacttg gggcagacgt tggcgttccg gaggacctct ttcttataga
      841 tgatttcctt gcaggagttg cacttgaccc agaggccttc gggaacctgg atgcgccggt
      901 gctcgaccgc ctgtttaggc ttgcgaatct tcttgaacca cgcgatttcc atcaggtccc
      961 cgaccctcgg ccaatccccc cgggggtgcc gcagatagta ccgacccccc cgaggcaggt
     1021 caaggcgaga gaactaccga ggctgaaacg agcccccgtg cgcgcttttc gaacactcac
     1081 ggggggcccc gtgaccagac tcccgcctcg acagcaagtc ttttattttt aatcagttgt
     1141 atgcgtgtcg gcgggcgcag gcgggcggtc gggatcgccc gcgtgtagct tccggacgcg
     1201 gtgcacccga cagtcgattg gcgatcggtc cctccggtcc tgaacaaaaa cgacagagac
     1261 tgtgcaagtt attgaatcaa agcatcttgc tgcgatggcg cgatgccatt ttagcactcg
     1321 cctccgactg gaacgggcgt tgcgataagg ggggcaggca tcgtgagatg cattcccaag
     1381 gagggtcgcg ggcgaaaagg tagggcgagc tgacacgcag ggaaacctgc acggagccgg
     1441 tcccgtctcc tccccacggg gccggctctc gtgctgcccg ggccagatga tgttaggggg
     1501 gcggggggtg cggaatgacc gacctgagcg ggttgctggg accggtgatg atggccgggg
     1561 cggcggccgt cctcggctat ctggccgccc gccgcaaaga caagcctcgg gccggccgag
     1621 ccgcccacgc gcgtttgcat ccatcagggg ccgccggggc ggccttgtgg gaggacgtta
     1681 gtcccgaacc gctcgaagcc acgctccagg ccctcatcca ggcgatcgac gcgcgcaacc
     1741 ccgcggcgcg cgggcacgtc cgccgggtcc gcgccgccgc gatggaactg ggccgcggtt
     1801 gcggcctcga cgcggaggcg ctcgcgacgc tgcggctcgc cgcgctcctg cacgacgtcg
     1861 gcacgctgat cgtccccgag cagattctca gcaagcccgg acgactcaac gacgcggagt
     1921 tcgacaaggt gaaggcgcat ccggtcgcgg gcgtcgaaat gctcgcgccg gcggggctgc
     1981 cggtcggcgt gctcgagatc atccggcacc accacgagcg ctgggacggc ggtggctacc
     2041 cggaccggct gaaggggatg gaaatccctc tgggggcgcg catcctggcg gtcgccgacg
     2101 cgttcgaagc gctcgtctcc gagcgcgcgc accgcggagg cctggccccc tccgaggcgg
     2161 tcgatctcat cttgacgtgg gccggggtcc agttcgatcg ggacgtcgtc gccgtcctgc
     2221 ggtccgaggt gacggccgtg cacgaggcga tggcggccgc ggatcacctc ccggagccgc
     2281 cgctcgcgat cgcgcgtctc gcgtcgcagt ccgccggcgc gccggagcag gcgaccagcg
     2341 atccgggggg attcgggatg ctcctgcacc gggggatctc cgccggcgga gccgcggctc
     2401 cctccggggc ggaggcgccg cgtgcgacgg cggccgtgtc gaggaccccg ggcgatgcct
     2461 ccgcggcgca gcgcgaggtc tacgccctgt acgagatcgc ccgcacgctc tgcaccagcc
     2521 tgcacctgac cgacgtcctc gacctcgtgg tcggcaagat cgccatgctg gtgccgttcc
     2581 ggacctgcgt cgtcttcctg accgccgggc cgagggagct gcaggcgcgg ttcgtgtccg
     2641 gggcgaacgc cgcctccctg cgcggacggc ggctggagta cggcgacggc atcaccggct
     2701 gggccgcaga gcaccggacc agcagtttct ccggaaccgc cgagctcgac ctgttcggga
     2761 gcgaggtcga tccggccgcc tactcgaccg tggccgcgtt ccccctcgtg cagggcgacg
     2821 aggtcctcgg cgtgatcacg ctctacttcc cggcgaacgg gccgtgccac gacgaccacg
     2881 tgcgcgtgat ggagatcctc gcgcggctcg ccgctggggc gatccacgag gggcgcctcg
     2941 gcctcgagca gggggagacg ggcctgaccg acgcggtcac gaaccttccg aacgagcggt
     3001 atctccggca ggcgttcgag aaggaggtcc tgcgcagcca gcagtccggg cagccgctga
     3061 gcctcgtcga gatggacctc gaggacttcc gcatcctcaa cgagcggatc ggcgcggagg
     3121 cgggggatcg cttcctgatg gaggtcgggc gcatcctgag aagtcatctg cgcgaacggg
     3181 acgtgctcgt tcgcctcacc gaggacggct acgcggcgct tctccccggg accggcttcg
     3241 ccgcggcggc gctgctgacc gagcgcctgc agcgggcggt ggacggcttc gtcctgcgcc
     3301 tggagacagg gggcacggcg cgcgccgggc tttcgatcgg catcgccatc cacccggtcg
     3361 atggcgaggg gctcgatgac ctgctgcagc gcgcttccat caaccggacg cgcaaccgcc
     3421 aggcccggcg ggcggcgcgc gcggagacgc ccaacgtcgt gccgttccgc cccggccatg
     3481 gtggtgccta gcctgtggcg ttcggcggtg gacggaaagt gatgacgcag cgttgccggt
     3541 gaacgcgccg acggagtcaa atccttgatc tcacgacagt tcccgaaaag cggtgcaggg
     3601 attcgatttt gcttgggtcc cggccatgcg ctggcccggg actcccgtgg aacgtttgca
     3661 agcgtttcgg ccggagttct gcccttggcc tgagtgccgc gagcaccgac ggacttctgc
     3721 cggctttcga tatcgacggc acggttactt caggactgcg cggcgaaagg tgccgcgatt
     3781 ccgatgcgcg tcatgcggcc gctcgttctc ccgacagtcg ttcgggacgt cctattacct
     3841 caagaggccg gagctcctgc tgccgatcgc cgcgggactc gtggccggat ctgcccaccg
     3901 gcagatggcg cgatcgttcg gatgcgctcc ctcgaccgtc actcggctga gcgcccggct
     3961 cgggaggcac acgctgctgc tccacgccct ggccctacgg cacctcgcgg gtcgactctc
     4021 ggagccgctc gcggccgacc acttcgagac gttcgagttc tcgcaggatt tcccgttcgg
     4081 agtcctgaca ctcgtggggc gcgattcgtg gttcgtctac ggtctcgacc cggcgccgca
     4141 cgcccgggga ggcaaggtca cgccatatca ggcggtgcgc ctcgcggccc ggccgaggat
     4201 cccgcgccgc ggcggctatc gcggctcctt ccgtagggga ctcgacgtgc tgttgccgct
     4261 ctcgcattcc acggtcgaga ttgcctgcga cggcaaggaa ccatacatgc tggctctccg
     4321 gaaacatccc cagcgcgacc gcatccggct cgaagtgcac ccgaatcctg tccgcggacc
     4381 caagggcgcc ccgcgctctc ggcaagccct tgcccgtgat cgcgcgatgt acgctgtcga
     4441 ccagctgcac atgctcctgc gccactcgcg cgccgaccac aaacgcgaga ccatggcctt
     4501 cggccggcgg ctcaatgcca tcctcgagcg cctccatctc gcgattgtct ggcgcaactt
     4561 catcaaggga cgctccgaaa gacgtcccga ccccgacact cccggaatgc gcctgggatt
     4621 gagcgattgt ccctggacat ggcgccgcgt gctcgcgcgt cgcctcttcc cttgccgcgc
     4681 cagcctgact cccgtcctcc gcgacctcta ctggcgcaac tggcgcacac ccatcctccc
     4741 caccaatcac ctccaccgcc tccgccacgc cgcctgaacg accagcaatg accttcccgc
     4801 gcattgcgcg catttccact tctacaagct ccgcaccacc atgtaagatg accccggtca
     4861 tgccggccgg acccgcggcg gccctcgccg ccctcaccct catcgtcaac gcctccgtcc
     4921 tcgacgggac cggcgccccg ccgaagaacg tcgcggtgcg gatcgacgcg gggcggatcg
     4981 tcgaagtcgg cgacctgaag ccgcgccccg gcgagaccgt catcgacgcg aaggggctga
     5041 gcctcgcccc cggcttcatc gacacccaca gccacgccga cgaggagctg ttcgagcacc
     5101 cggacgcgct cgccgacgtc agccagggga tcacgaccat cgtcgtcggg caggacggcg
     5161 gctccccctt tccgctgaag aagttcttct cgcggctcga gcggaagccg gcggcggtga
     5221 acgtcgcgtc gtactcgggg cacgggacgc tgcgtgaccg ggtgatgagg gaggacttcc
     5281 ggcgcaaggc gaccgcggag gaggtccgga agatggagcg cctcctcgac aaggacatga
     5341 aggccggggc gctcgggctc gcgaccggcc tggagtacga cccgggcatc tactccgatc
     5401 cgtccgaggt gatcgcgctc gcgaaggcgg cggcgcggca cgccggccgc tacatcagcc
     5461 acgtccgcag cgaagatcgg aacttctggg aggcgatcga ggagctgctg accatcgggc
     5521 gggacgcccg gctgccggtg cagatctccc acaccaagct ggcgatgaag tcgtggtggg
     5581 gccagtcggc gcggctcctg aagcgactcg acgaggcgcg cgcctccggg gtcgacgtca
     5641 ccgccgacat ctacccctac ctctactggc actcgacgct gaccgtgctc ttcccgaagc
     5701 gcaacttcga ggacatggag gaggcgcgcc tcgtcgtcaa cgagatcgcg cctccggacg
     5761 ggctcatcct gacgcagtac gacgcggagc cgacctgggt ggggaagagc ctggcggagg
     5821 tggcggcgtc gcgcaacgag gacccggcgg tcaccctcgt catgctggtg cgccgcgcgc
     5881 tggagcacga gaaggagacc ggcgacgccg gcgagggggt gctcggcacc agcatgaccg
     5941 aggaggactt cgaggcactc ctgcgctggc cgcacatgaa cctgtgcacc gacggcgcgc
     6001 tcgaaggctc acacccgcgc ggcttcggcg ccttcccgcg ggtgttcggc cgctacgtgc
     6061 gcgagcgcca cgtcctgacg ctcgaagagg cggtgcgccg cgccagcgga ctggccgccg
     6121 agaacatggg gttccgggat cgcggcctca tccgccccgg gatggcggcc gatctcgtgc
     6181 tgttcgaccc ggacacgatc gccgaccgcg ccacgatcac cactccgcac gccctctccg
     6241 agggcgtgaa caccgtgtgg gtcaacggcg tcgtcgtctt tcaggaaggg cgcgccaccg
     6301 gcctcaggcc cgggtcggtc ctcaggaact tcagcaccca aggcgacagg acctcgttac
     6361 cgtagtcggt gtagtggcag tggtccgcga ggatcggccg cggcgccttc ggcacggtct
     6421 ctctcaggcg gggcagcagg tcgaggagcg gcaggtcgag cgagcgggcg atcgccccga
     6481 gccggcgctg cggctccgag gcgtcgaagc gggcctgcac ctgggcggtc gccgggaagg
     6541 cgaggagcat cggccggaag cgatgttcgg ccgagagcct cttgaactgc atcaggagcg
     6601 gctcgatccg cccccaggcc gaatcggtcc aggcgctccc ccagtcgaag taggacgcga
     6661 gcacctcggc gttgaacgcc ggacgatcga ccgccggatc gcccgggccg ggcgggtaca
     6721 gccggcgcag ctcggcgcgc caggcgccgg tctcccatga ttcgccgctg aacgactccg
     6781 gctcgagcag cgccgggatc atcgcgccgg cctgccgcgc cagccaggac catcgcagga
     6841 gcgccggcgg ctgcatcagg cgcacgccgc gcgacgcctc ggcgtcattg aggaagaagc
     6901 cgatcaccac gatgtccggc gcggtcgaga ggccggtctc ctcgaggatg gcgatctcgt
     6961 tggcgagccc gatcccggcg accgccgcgt tgatcgtctc gaacggcggc ccgccgtcct
     7021 gctccgacag cgcctgcacc cggcgcacga acgacgcctc ctcgggaagg tactgcgaca
     7081 aggtcaccga gtcgccgagg aacaggacgc gcggcttcgt cttcgggccg atctcggggt
     7141 tgcggtatcc gagcgagttg gtgcggacga tgatgtccgc ggtcgccccc gggtagttct
     7201 ggatgcgagc ctccgaggag gcgcgcatgc gcagcccggc cggcgtctcc agcacgaagc
     7261 cgtacggccc gaggtccccc tgcgtgacga cgcgcaggct cttcggcgcc cgggtctcgg
     7321 cggcccggcg cgtcaccgcg tccgtccggg cgatgacgac ggtgggaggc ggcgggaggg
     7381 cgacccgcac gacgacctcc gccaggagca ggccggcgat cgtcccgccg aggatcaaca
     7441 acaggcgcgg gaggatctcc gtggtgcgcc cgctcacgcg gcaggctcgt tcaagaagcg
     7501 cgcgcgatca gaatggggcc gactcccgcc cgcaggaatc gaccgcggtc acgacgtaga
     7561 agaggttctg atcggtcagg agccccgcac ccgtgtggac gaacgatttg gtggtggtgc
     7621 tcccggcccg gacgagggtg gcgaggccgg accccgatcg catgtaaacc tcgtacgccg
     7681 aggcattcac cgggtcggcg ctccagtcga ggacgatgtc gtcgtcgctg cgattgacct
     7741 ggatcgtccc gacggtcgcg accgggccgc tgcagacgct gcagccggcc cccggctggt
     7801 acagcgagac gtcgtcgaat cccgcctcga cgagcgagcc gccgaccccg agatcctcgg
     7861 cggtcacccg gacccggaac tggttgttga aggggatgag gccgtcgagg acgagcggcg
     7921 tcgtcgtcca ggaagcgatg tcgcccgtcg tctgcaacac cggtgtccag gaggctccgc
     7981 cgtttccgga gacgtgcacg cggtacggat cctcgcccgg attgttcccg ccggagttgc
     8041 tgtagaagag cgccatctcg agcacgacgc tcgtgcgtcc ggtgagatcg aggatcggcg
     8101 agacgaccgt cgtcgtgc
//
LOCUS       DASRIQ010000324         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1696981, whole genome shotgun sequence.
ACCESSION   DASRIQ010000324 DASRIQ010000000
VERSION     DASRIQ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696981"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..2181
                     /locus_tag="VFD06_07790"
     CDS             268..2181
                     /locus_tag="VFD06_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN03478.1"
                     /translation="MERDVREGGVVTHIGRDLSVALAAWVAIVLLFGVTRALGQERIA
                     ARDLGDLSLEQLSNIVVTSVSGRTEPISRSLASVFVITGDEIRRSGANSIMEALRLAP
                     NLQVAQTGADGYAISARGFNDTLANKLLVLIDGRSVYTPTFSGVFWDAQDVMLEDVDR
                     IEVISGPGGVLWGVNAVNGVINIMTKRSADTQRWLVSAGGGNREQRVSGRYGGALESG
                     HWRAYAQGTRRENTETPDGTENDDGIGRVQGGSRFDWKRGADAFTVQGDVYDQRDNGE
                     HPNTSELRGANLLGRWNRDLRSGDSLHVLAYWDHTERTSQRLDTGSVEFRHALHERRG
                     HRLLWGGGLRQDRDRIENGASVAFIPADKDLNSWNIYGLDEIALGPDVDLSLGAKVDR
                     NSYTGEEFLPSVRIGWRPVDEHLLWAAWSRALRTPSRFDRDLFIPGAPPFLLIGSDHF
                     DSEISSVYEIGYRGQPSERFSWSATVWYDDLEKQRSIAPGAAGATVENDLEGHSQGVE
                     TWGSYRVVDRWRLMGGYTHLEKELEPVEGTVDLQPSTALGSDPNEFWKLRSVLDLGRD
                     WEIDVMARYYGALENRDVPSYLAVDARVGWVLRERLELSLLMRNLTDAGHIEWSPGAE
                     FDRTFFLNALIRF"
BASE COUNT          385 a          811 c          796 g          354 t
ORIGIN      
        1 gcgcggctcg gggcaggggg agaccaccac gaacctggac aagatgaaca agaagatcct
       61 caccctgatc acgaagatgg ccgaccgctg gcgcgagatg cgcgccgacc acgagcggga
      121 caaagcgaag gcagccgcca aaggctgatg ctccacggcg tctcgccggc ccgcccacag
      181 gccggccgtt cccggtaccg gaaatcacgg atgttcccgc cccgttccgg cgcgtagaat
      241 tccgcgctat tcacgcgaac ttctggcgtg gagcgggacg tccgggaggg gggggtggtg
      301 acgcacatcg gccgggatct gtccgtcgcg ctcgcggcgt gggtcgcgat cgtcctgctt
      361 ttcggcgtga ctcgcgcgct cggccaggag cggatcgccg cgcgcgatct cggggacctc
      421 tccctcgaac agctgagcaa catcgtcgtc acctcggtga gcggacgcac ggagccgatc
      481 tcgcgatccc tcgcgtcggt gttcgtcatc accggggacg agatccggcg ctccggcgcc
      541 aacagcatca tggaggcgct gcgcctcgcc ccgaaccttc aagtcgcaca gaccggcgcc
      601 gacggctacg cgatctcggc gcggggcttc aacgacaccc tcgccaacaa gctcctggtc
      661 ctcatcgacg gccgcagcgt ctacacgccg accttctccg gagtcttctg ggatgcccag
      721 gacgtgatgc tcgaagacgt cgatcggatt gaggtgatca gcggtccggg cggcgtgctg
      781 tggggggtca acgcggtcaa cggcgtcatc aacatcatga cgaaacgctc cgccgacacg
      841 cagaggtggc tcgtgtccgc gggcggcggc aaccgtgagc agcgggtctc gggaaggtac
      901 ggcggtgccc tcgagagcgg ccactggcgc gcctacgccc aggggacccg ccgcgagaac
      961 accgagacgc cggacggcac ggagaacgac gacggcatcg ggcgcgtgca ggggggctcc
     1021 cgcttcgact ggaaacgcgg cgccgacgcc ttcacagtgc agggggacgt ctacgaccag
     1081 cgggacaacg gggagcatcc gaacacctcg gagctgcgtg gcgccaacct cctcgggcgc
     1141 tggaaccgcg acctccgcag cggcgacagc ctgcacgtgc tggcgtactg ggaccacacc
     1201 gagcggacct cgcagaggct cgataccggt tccgtcgagt tccgtcacgc gctgcacgag
     1261 cgtcggggcc accggctcct ctgggggggc ggcctgcgcc aggatcggga tcggatcgag
     1321 aacggcgcca gcgtcgcctt catcccggcg gacaaggacc tcaactcctg gaacatctac
     1381 gggctcgacg agatcgccct cgggcccgac gtcgacctct ccctcggcgc caaggtcgat
     1441 cggaactctt acaccggcga agaattcctg ccgagcgtcc ggatcgggtg gcgtcccgtg
     1501 gacgaacatc tcctctgggc cgcctggtcg cgcgccctgc gcaccccctc ccgcttcgac
     1561 cgggacctgt tcatccccgg cgcccccccg ttcctgctga tcgggagcga ccatttcgac
     1621 tcggagatct cctccgtgta cgagatcggc taccggggcc agccgtcgga gcgcttctcg
     1681 tggtccgcga cggtctggta cgacgatctc gaaaagcagc ggtccatcgc cccgggggcg
     1741 gccggcgcga ccgtcgagaa cgatctcgaa gggcactcgc agggggtgga gacgtggggg
     1801 tcgtaccgcg tcgtcgaccg ctggcgcctc atgggcgggt acactcacct cgagaaggag
     1861 ctcgaaccgg tggaagggac ggtcgacctg caaccctcca ccgccctcgg ctcggacccg
     1921 aacgagttct ggaagctgcg gtcggtcctc gacctcggca gggactggga gatcgacgtc
     1981 atggcgcggt actacggcgc cctggagaac cgcgacgtcc cctcgtatct cgcggtggac
     2041 gcccgcgtcg gctgggtcct gcgggagcga ctcgagctgt cgctcctgat gcgcaacctg
     2101 accgacgccg ggcacatcga gtggtccccc ggagcggaat tcgaccgtac ctttttcctc
     2161 aacgccctga ttcgcttcta ggagcccggc ccgagtgcgt acgttccatc gcttggtcgt
     2221 cctcgcggca gcaaccggac tcctgggcac gggcgtggcg cgggctcaga tggagcacga
     2281 ggtcaaggcg gcccacctct tcaagttcct gtcgtacgtc gactggccgg ccgccgcgtt
     2341 cgaagg
//
LOCUS       DASRIQ010000325         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_908356, whole genome shotgun sequence.
ACCESSION   DASRIQ010000325 DASRIQ010000000
VERSION     DASRIQ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908356"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFD06_07795"
     CDS             <1..354
                     /locus_tag="VFD06_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN03479.1"
                     /translation="ARRMIDAGVALALATDFNPGTCPTEAMPPILSLACLRYALHPAE
                     AIAAATVNAAHALGLGADRGSLEPGKRADFQVADVPNHLHLAYRTGVNHCRAVVAGGR
                     LVVEDGRLTPAASGG"
     gene            392..1036
                     /locus_tag="VFD06_07800"
     CDS             392..1036
                     /locus_tag="VFD06_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HZN03480.1"
                     /translation="MGLAMPLLRDLTIAGFLERLAAGTPTPGGGSAAALAGALGGALI
                     QMVCRLTIGRDEARPHEAALGALLGQAADLEARLRDLVDEDAQAYDEVMQALRMPKGT
                     DDEKAARRLALRRANAAATDTPLRTAEACHLLLGLAAELAGTGNRNALSDIGSAAQLI
                     QAGLRGAVMNVRINLPGLGEPAQAEQFETRVRTLEEEGTRRLVLCLERLAARAR"
     gene            1465..>3431
                     /locus_tag="VFD06_07805"
     CDS             1465..>3431
                     /locus_tag="VFD06_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03481.1"
                     /translation="MSRSASVRSTLGVFLASVVTVGLLVSPYALTWGSREKVGSSPGE
                     LAEQNEWRKFRQLPRFTAVERVADLSPFGPTFQPDAPNSDARARRNLIETAIGYVDLK
                     KADQGVTKRLPAALRHDAASVRAAGSKGRGRVGDVNIVQVSADALQARGADDIESAIR
                     SHGRLLEQVPDRGYLVRTPNEGALAALAAEPFVEVMAVWEPGYKIAPSTGRMPLLERK
                     RARSNDLDLIVSLWSDADPEAARGALEAIAGKGKVSSWSVDGTVLNVKASVQTSQRLA
                     RDPSVRSLSERREFVLTNSETPTLLMLGQTEFSDGMARPYHEVGVDGGGIDTNLDGRR
                     LNDGTDAVPPQIVVVTDNGISVDAVHFSQTLTQTTTITNPIGPAHRKVHNIQNVTDSG
                     LSCDGVLSGSNTHGNVVAGIIAGAPGDFGLTFSKAIDPADGPPVTGVSMDALARGSRI
                     IMQDAGIQTQCLLSELVESGGNISPGNILDRLNLGAIGGGSESHLHILPFGVPNFDNI
                     LGNLENGTYTLESHQVDLFLVNNRDYMVFSPVGSQGSRINGLNFEEVWPDLFDGTALD
                     NDPNDQKPLQVPPPATAKNPVTVGGSSSDLWTVFGDYNDEENPLGFSSKGPARPRRCA
                     PCRWSPRRAPTDPACSPTRCSTPPRPTAAATT"
BASE COUNT          521 a         1273 c         1171 g          466 t
ORIGIN      
        1 gcccgccgga tgatcgatgc gggagtggcg ttagcgctcg cgaccgactt caaccccggg
       61 acctgcccga ccgaggcgat gccgcccatc ctgtcgctcg cctgcctgcg ttacgcgctg
      121 cacccggcgg aggcgatcgc cgccgcgacc gtcaatgccg cgcacgcgct cgggctgggg
      181 gccgatcgcg gcagcctcga gccgggcaag cgtgccgact tccaggtcgc cgatgtgccg
      241 aaccacctgc acctcgccta ccgcaccggc gtgaaccact gccgcgccgt cgtggccggg
      301 gggcggctcg tcgtcgaaga cggccggttg acgcccgcgg cgagcggggg atagagtccg
      361 cgccggccgc ggcctccgcc gcgacgccgc gttgggcctg gccatgccgc tgctgcgcga
      421 cctgaccatc gccggtttcc tcgagcgcct cgccgccggg accccgaccc cgggcggcgg
      481 cagcgccgcc gcgctcgccg gcgccctcgg cggcgccctg atccagatgg tctgccggct
      541 gacgatcggg cgcgacgaag cccgcccgca cgaggcggcc ctcggggccc tcctggggca
      601 ggcggccgac ctcgaggcgc gcctccggga cctcgtcgat gaggacgcgc aggcctatga
      661 cgaggtgatg caggcgctgc gcatgccgaa agggaccgac gacgagaagg cggcgcgccg
      721 cctggcgctg cggcgcgcca acgcggcggc caccgacacc ccgctccgga cggcggaggc
      781 ctgccacctc ctcctggggc tcgcggccga gctggcgggg accggcaacc gcaatgcgct
      841 gagcgacatc ggcagcgccg cccagctcat ccaggcgggc ctccgagggg cggtcatgaa
      901 cgtccggatc aacctgcccg ggctggggga gccggcccag gcggagcagt tcgagacgcg
      961 cgtccggacg ctcgaggaag aggggacgcg gcgcctggtt ctctgcctcg agcgcctggc
     1021 ggcgcgggcc cgctgatcgc gatctctgcg gctcctcccc ccgccgcccg ggcgcgccag
     1081 atggaaccgc agtcccgcat cccgaaacgc caccgtgcgc cgcgatccgc ccggaaacgg
     1141 gcgtcaagtc tcacatcgcg ggacaaatcc gaatttcgtt gcgccacagg gtggaacaat
     1201 ttaggttgac ctggttcccg tcggattttc gggaaatgag tggttgacag ttccgaggtc
     1261 gtaccgtact caatgaccca cattcgttgc aaccccgggg ggggaaggcg aaggctgtct
     1321 tcgccccctg aattccccgg ggcggtcgag gatggcggat aaaacctgca ccccacatcc
     1381 cgagcgtcgg acggcccacc cgtccgacgc gcggccggca ccggggggtc aacccggcgc
     1441 ctcttcccca cgcgggaggt tctgatgagt cgttctgctt ccgttcgttc cacgctggga
     1501 gtgtttctgg cttccgtggt gaccgtcggc ctcctggtga gtccgtacgc gctcacctgg
     1561 ggctcgcgag agaaggtcgg gtccagcccc ggtgagctcg ccgagcagaa cgagtggcgc
     1621 aagttccggc agctgccccg cttcacggcg gtggagcgcg tcgcggacct gtcaccgttc
     1681 ggccccacct tccagccgga cgcgccgaac tccgacgccc gggcgcgccg gaacctgatc
     1741 gagacggcga tcggctacgt cgacctcaag aaggccgacc agggcgtgac gaagcgcctg
     1801 ccggcggccc tgcggcacga cgccgcgtcg gtccgtgccg ccggctcgaa agggcgcggg
     1861 cgcgtggggg acgtcaacat cgtgcaggtg agcgccgacg cgctgcaggc gcgcggtgcc
     1921 gatgacatcg agagcgccat ccgctcccac ggccgcctgc tcgagcaggt gccggaccgc
     1981 ggctacctcg tgcgcacgcc gaacgagggg gcgctcgccg ccctcgcggc cgagccgttc
     2041 gtcgaggtca tggcggtctg ggagccgggc tacaagatcg ccccgtccac cggccgcatg
     2101 ccgctcttgg agcgcaagcg cgcccgctcg aacgacctcg acctgatcgt ctctctctgg
     2161 tccgacgccg atcccgaggc cgcccgcggc gccctcgaag cgatcgccgg caaggggaag
     2221 gtgagctcct ggagcgtcga cggcacggtt ctcaacgtga aggcctccgt gcagacgtcg
     2281 cagcgcctgg cgcgggaccc gagcgtccgc agcctgtcgg agcggcgcga gttcgtcctg
     2341 accaactccg agacgccgac cctgctgatg ctggggcaga ccgagttctc cgacggcatg
     2401 gcccgtccgt accacgaggt gggcgtggac ggcggcggga tcgacaccaa cctcgacggt
     2461 cgccggctca acgacggcac cgacgcggtg ccgccgcaga tcgtcgtggt caccgacaac
     2521 ggcatctcgg tggacgcggt gcacttctcg cagaccctga cgcagaccac gaccatcacg
     2581 aacccgatcg ggccggcgca ccgcaaggtg cacaatatcc agaacgtcac cgactcgggc
     2641 ctctcctgcg acggggtcct gtccggcagc aacacgcacg gcaacgtggt cgccgggatc
     2701 atcgccggcg ccccgggcga cttcggcctg acgttctcca aggcgatcga tccggcggac
     2761 ggcccgccgg tcaccggcgt cagcatggac gccctggcgc gcggctcccg catcatcatg
     2821 caggacgccg gcatccagac gcagtgtctg ctgtccgagc tggtcgagtc gggcggcaac
     2881 atttcgcccg ggaacatcct ggatcggctc aacctcgggg ccatcggcgg cggctccgag
     2941 tcgcacctgc acatcctgcc gttcggcgtc ccgaacttcg acaacatcct cggcaacctc
     3001 gagaacggga cctacacgct cgagtcgcac caggtcgacc tgttcctcgt caacaaccgc
     3061 gattacatgg tcttctcgcc ggtcggcagc cagggaagcc gcatcaacgg gctcaacttc
     3121 gaggaggtct ggcccgacct gttcgacggc accgccctcg acaacgaccc caatgatcag
     3181 aagcccctcc aggtgccgcc cccggccacc gcgaagaacc cggtgacggt cggcggctcc
     3241 agctccgacc tgtggacggt gttcggcgac tacaacgacg aggagaaccc gctcggcttc
     3301 agcagcaagg ggccggcacg gccgcgtcgc tgcgcaccgt gccgctggtc accgcgccgg
     3361 gcaccgacgg atccggcctg ttcgcctacc cgctgttcaa cgccgccgcg accaaccgca
     3421 gccgcgacaa c
//
LOCUS       DASRIQ010000326         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1444379, whole genome shotgun sequence.
ACCESSION   DASRIQ010000326 DASRIQ010000000
VERSION     DASRIQ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444379"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1143
                     /locus_tag="VFD06_07810"
     CDS             100..1143
                     /locus_tag="VFD06_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZN03482.1"
                     /translation="MTRSTVPVRTFPRALAAAALLTAATLAAPARAAIVEEIVAKVNN
                     RIITKSEYEQRSLYLVQQTKGSEDEMQAAKDALLANLITEALLIERAETIFDMERIRT
                     SLIEDFRKQQNIANDEELETALKDQGMTRKELEEHLMRLAVPNEIINYDVKRKISVSD
                     AEMQAFYDQHRSRWETPTTVTLREIVVTYVPETRDEARARARGLATQAREGGDFIEMV
                     KESSEGGTREVEGILGPVAAKDLHPALNQAALALKIGEISDPVDTGRALHIVKLEARV
                     ETTVKTLADVKDEVYNAVREEKFRPKFDNYLKKLWKENYIEVEPKYEPLLVISPLKKP
                     VAGAAAGAAAGAP"
     gene            1347..1745
                     /locus_tag="VFD06_07815"
     CDS             1347..1745
                     /locus_tag="VFD06_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03483.1"
                     /translation="MRRYEPGNTAPPAHTERKPDDGAGHRRLIRPNLSELKDRLGPRG
                     APRRKQVPPAETNAEAFYYLKQMNTRTPMVVVMKDGEVLHGVIEWYDRTCIKVNRVGS
                     PNLMVMKDCIKYMYKEEETRGVKDSTGDNA"
     gene            1840..2949
                     /gene="pdxA"
                     /locus_tag="VFD06_07820"
     CDS             1840..2949
                     /gene="pdxA"
                     /locus_tag="VFD06_07820"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZN03484.1"
                     /translation="MLDVCRPLLIGDHAHLLREAARLRGAGAWNPHTDGVWADVGDPD
                     RWPEVAKPAFDEIGSKQGAEFVVESWKEGPAFHDMGALDPDEPAAAGAPAGTDAAPPG
                     GGPTTSETEAGRTPTASGTEAGRGPTASGGRASVKYIKQAVGLLRTGAAAALVTAPIS
                     KTSLRLASIHYPGHTELLADLSGAEQDEVVMLFVTPQYKTALLSMHLPLKEAISGLNQ
                     ELIVKRLAVLHVEHGRWFGADPKIGVAALNPHAGEEGLFGQEERDILAPAIDAAREAG
                     IDARGPYPADTIFARAARGEFDCVLALYHDQATIAVKTASFGQAVNMTLGLPFLRTSV
                     DHGTAWDIAGQGKADPSSLVEALRLAARLAPRVHR"
BASE COUNT          568 a         1085 c         1060 g          403 t
ORIGIN      
        1 gctatctcgc cgacctgaag gagaagaccg aagtccgtgt gcaccgcgcc ctgctgccgt
       61 tccgctacgt cggcgaattc cagagctagt cgttccgcca tgacgcgttc caccgtcccc
      121 gtccggacct ttccgcgcgc gctcgcggcg gcggcgctcc tgacggcggc cacgctcgcc
      181 gcgcccgcgc gggcggcgat cgtcgaggag atcgtcgcca aggtgaacaa ccgcatcatc
      241 accaagagcg agtacgagca gcgcagcctg tacctcgtgc agcagaccaa ggggagcgag
      301 gacgagatgc aggccgcgaa ggacgcgctc ctcgccaacc tgatcaccga ggcgctcctc
      361 atcgagcggg ccgagaccat cttcgacatg gaacgcatcc gcaccagcct catcgaggac
      421 ttccgcaagc agcagaacat cgccaacgac gaggagctgg agacggcgct caaggaccag
      481 gggatgacgc gcaaggagct cgaggagcac ctgatgcgcc tggccgtgcc gaacgagatc
      541 atcaactacg acgtcaagcg gaagatcagc gtcagcgacg cggagatgca ggccttctac
      601 gaccagcacc gctcccgctg ggagaccccg accacggtga cgctccggga gatcgtcgtg
      661 acctacgtgc cggagacgcg cgacgaggcg cgggcgcggg cgcgcggact cgcgacccag
      721 gcgcgcgaag ggggggactt catcgagatg gtgaaggagt cctccgaggg gggaacgcgc
      781 gaggtcgaag ggatcctcgg gccggtggcg gcgaaggacc tgcatcccgc gctgaaccag
      841 gcggcgctgg cgctcaagat cggcgagatc agcgacccgg tggacaccgg ccgcgccttg
      901 cacatcgtca agctggaggc gcgggtcgag acgaccgtca agacgctcgc ggacgtcaag
      961 gacgaggtgt acaacgccgt ccgcgaggag aaattccgtc ccaagttcga caattacctc
     1021 aagaaactgt ggaaggagaa ttacatcgag gtcgagccga agtacgagcc gctcctcgtc
     1081 atctcgccgc tgaagaagcc ggtggccgga gcggctgccg gagcggctgc cggagcaccc
     1141 tgatgacgac cacgcggccg ggcgtgagcg cgacggtgcc agaggtcaca ctgtggcggc
     1201 gtgttttcgg ctgccggctg tgatatgttg agaactaatc ggacgctccc cgagcccgaa
     1261 atcgccacaa gatgaaatct gaccaagggc cccccgacca tatgaacggc ccggcgcccc
     1321 gccgccgcgg cccaagaggg aatctcatgc gacgttacga accgggcaac accgcgccgc
     1381 cggcccacac cgagcgcaag ccggacgatg gggcggggca ccgccggctg atccggccca
     1441 atctctccga gctcaaggac cggctcggtc cgcgtggagc gccgcgccgc aagcaggtcc
     1501 ccccggccga gaccaacgcc gaggccttct actacctgaa gcagatgaac acgcgcacgc
     1561 cgatggtcgt cgtcatgaag gacggcgagg tgctgcacgg cgtcatcgag tggtacgacc
     1621 gcacctgcat caaagtgaac cgcgtcgggt ctcccaacct catggtgatg aaggactgca
     1681 tcaagtacat gtacaaagag gaagagaccc gcggtgtgaa ggactccacg ggcgacaacg
     1741 cctgacgccg gtcccctgaa gccgatcctc gcggtctcga ccggcgatcc ccacggcatc
     1801 ggtccggaaa tctgcctgaa ggcggctgtc cgcccggaag tgctggacgt ctgccgcccg
     1861 ctgctcatcg gcgatcacgc tcatctcctg cgcgaagcgg cgcggctgcg cggcgcgggc
     1921 gcctggaacc ctcacaccga cggcgtctgg gcagacgtcg gcgatccgga tcgctggccg
     1981 gaggtggcca agcccgcctt cgacgagatc ggctcgaagc agggggcgga attcgtcgtc
     2041 gagtcctgga aggaggggcc cgccttccac gacatgggcg cgctcgaccc cgacgagccg
     2101 gccgccgccg gcgcccccgc gggcacggac gcggcgcccc cgggcggggg tccgaccacg
     2161 tctgaaacgg aggcgggccg cacaccgacg gcgtcgggga cggaggcggg ccgcggtccg
     2221 accgcatcgg ggggacgcgc atcggtcaag tacatcaagc aggcggtggg acttctcagg
     2281 accggcgccg ccgccgcgct cgtgaccgct ccgatcagca agacgtcgct gcggctcgcg
     2341 tcgattcact accccgggca caccgagctg ctcgcggacc tgagcggcgc ggagcaggac
     2401 gaggtggtga tgctcttcgt gacgccgcag tacaagacgg cgctcctgtc gatgcacctg
     2461 ccgctcaagg aagcaatcag cggtctgaac caggagctca tcgtgaagcg gctggccgtc
     2521 ctgcacgtcg agcacggccg ctggttcggc gccgacccga aaatcggcgt cgccgccctc
     2581 aacccgcacg ccggcgagga agggctcttc ggccaggagg agcgcgacat cctggccccg
     2641 gcgatcgacg cggcccgcga ggccggaatc gacgcgcgcg gtccgtaccc cgccgacacg
     2701 atcttcgcgc gcgccgcgcg cggcgagttc gactgcgtcc tggcgctcta ccacgaccag
     2761 gcgacgatcg ccgtgaagac cgcgtcgttc ggccaggcag tcaacatgac cctcggcctg
     2821 ccgttcctcc ggacctccgt cgatcacggc accgcctggg acatcgccgg ccagggaaaa
     2881 gccgacccct cgagtctcgt cgaggcgctc cgcctcgccg cccgcctcgc cccccgcgtg
     2941 catcgttgac ctgcgcgcat ccgcgcgcga ggctaacgtc gcgcgcttac ttgcggtagg
     3001 cggcgcgcac gacgcgcagc atgtcggagt gggtctcggg cgaggcggcg atgatgtcgc
     3061 cgctctcgac gtagccggcg cggccggtga aatcggtcac caccccgccc gcctcg
//
LOCUS       DASRIQ010000327         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_909166, whole genome shotgun sequence.
ACCESSION   DASRIQ010000327 DASRIQ010000000
VERSION     DASRIQ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909166"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFD06_07825"
     CDS             <1..186
                     /locus_tag="VFD06_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03485.1"
                     /translation="AYPPNHLALGEALSKTGDASGARREYARARALALRAEEAGDPDA
                     GDWMREADEAIREAPGT"
     gene            236..2467
                     /locus_tag="VFD06_07830"
     CDS             236..2467
                     /locus_tag="VFD06_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HZN03486.1"
                     /translation="MSLVILVVAASSSGALAVPDRPAQELVTRFANLATTSLPRDFVE
                     PEGRTRWDLRALTPRDRFIRRVTDDLVSLRLVDDEPTGPRAWRFVDPDGGDAALRLLR
                     PDRQGRSLLPGTRAQFRIDRIAEDPARRGPEERTRFLVTSTIAGIGWAQLPSGPREVV
                     LERLLVLRASGAGFVPDRLVHRFVDPRAGVVAEIGGPPTSDGRGRVTVSEARVAEAIL
                     AGAADLRLYVRDVYSNPLSDIVYSRDRGAGTPVSAFTPAPGVTTIGQLIALDSWDFSG
                     NTTGLEVGATTAPMNAQETCNIAQCGYSVPGVVLDRTDRSFDVPANLDKINTALELEV
                     RPADTVIWMRAGAQHEGKAGSLGSGESRFCYTTFDGVTRTPAPLWLLPHEDSPGAERY
                     MLPGDTWSSVPFNCEQNLYNQVCGASQLFDRLWTKACDPSGADPLHNGTQSGATIKNG
                     VVTLPSGHTFNTMLARNVADFCVWAGAGCTTGFQVQAVRTVNYLWQIPVIGTVARLQS
                     VINAPDLTSWTSVQEGIVAYGLFPPRTIQVTAAGDTTVNLSWDPGLDTHRITGYKVYW
                     DTDSGGATPYAFNSAANPGQAAIAGTTAILSGLTPGTPYYFTVTALTSFTSPSSGLTT
                     SYESPLYPTQVSGDPDFVYPVEVTATTTGGVCLPVSQVANLQVSHAAGGDITLCWDPL
                     NDPCLAGYRILGATTPESAANYTTVADTDLGTCWTGNPSQRFFLVVARGTGGTGPWGH
                     YGQ"
     gene            2446..>2781
                     /locus_tag="VFD06_07835"
     CDS             2446..>2781
                     /locus_tag="VFD06_07835"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN03487.1"
                     /translation="MGPLRPVGARPLRRIGRLLLSLALLVGGGAFPARAQSVTGSLTG
                     TVRDAAGAPLAGATVIARSLETGAERSILSDREGRYRFELLTPGSWTVSARLGGDPPG
                     QTIVASIVLQ"
BASE COUNT          388 a         1039 c          937 g          417 t
ORIGIN      
        1 gcgtatccgc cgaaccacct cgccctgggc gaagcgctct cgaaaaccgg cgacgcgtcc
       61 ggagcgcggc gcgaatacgc gcgggcgcgg gcgctggcgt tgcgcgccga ggaggcggga
      121 gacccggatg ccggggactg gatgcgcgaa gcggacgagg cgattcgcga ggctcctggg
      181 acataatgcg ccacggaggt gctccatgat cgccgcccgt gcaaggaatg cccgtgtgtc
      241 tctggtgatc ctcgtggtcg cggcatcgtc gagcggagcg ctcgccgtcc cggatcgccc
      301 cgcccaggag ctcgtgaccc gcttcgcgaa cctcgcgacc acgtcgctgc cgcgcgactt
      361 cgtcgaaccg gaaggccgga cccgctggga tctgcgagcg ctcacgccgc gcgatcgctt
      421 catccgccgc gtgaccgacg acctcgtttc gctgcggctc gtcgacgacg agccgaccgg
      481 cccgcgcgcc tggcgcttcg tcgatccgga cggcggcgac gccgccctcc ggctgctgcg
      541 gcccgatcgg cagggacggt cgctccttcc ggggacgcgg gcgcagttcc gtatcgatcg
      601 catcgccgag gatcccgcgc ggcgcggtcc cgaggagcgg acccgcttcc tcgtcacctc
      661 gaccatcgcc ggcatcggct gggcgcagct gccgtccggg ccgcgcgagg tggtgctgga
      721 acgcctgctc gtcctgcgcg cttccggcgc cggcttcgtc cccgatcgcc tggtgcatcg
      781 cttcgtcgat ccgcgcgccg gcgtcgtggc ggagatcggc ggccccccca cgtccgacgg
      841 ccgcgggcgc gtcaccgtca gcgaagcgcg cgtcgccgag gcgatcctcg cgggggcggc
      901 cgacctgcgc ctttacgtcc gcgacgtgta cagcaatcct ctcagcgaca tcgtctacag
      961 ccgggaccgc ggcgccggga ccccggtctc cgcgttcacg ccggccccgg gcgtgaccac
     1021 gatcggccag ctgatcgcgc tcgacagctg ggacttctcc ggcaacacca ccggtctcga
     1081 ggtcggagcg accaccgccc cgatgaacgc gcaggagacc tgcaacatcg cccagtgcgg
     1141 ctactccgtg ccgggggtgg tgctcgatcg caccgaccgg agcttcgacg tccccgccaa
     1201 cctcgacaag atcaacaccg cgctcgagct cgaggtgcgg cccgccgaca cggtgatctg
     1261 gatgcgcgcc ggggcgcagc acgaaggcaa ggccggatcg ctcggctcgg gcgaaagccg
     1321 cttctgctac acgaccttcg acggtgtgac gcggacgccg gcgccgctct ggctcctgcc
     1381 gcacgaggac tctcccgggg cggagcggta catgctcccc ggagatacgt ggtcgagcgt
     1441 tccgttcaac tgcgagcaga atctctacaa ccaggtctgc ggagcgtcgc agctcttcga
     1501 tcggctgtgg accaaggcgt gcgatccgtc cggcgccgat cccctgcaca acgggacgca
     1561 gtccggcgcc acgatcaaga acggcgtggt gacgctgccg tcggggcaca ccttcaacac
     1621 gatgctggcg cgcaacgtcg ccgacttctg cgtctgggcc ggcgccggct gcaccaccgg
     1681 gttccaggtg caggcggtgc ggacggtcaa ctacctctgg cagattccgg tgatcgggac
     1741 ggtcgcgcgc ctgcagtcgg tgatcaacgc accggacctg acctcgtgga cttccgtgca
     1801 ggaggggatc gtggcctacg gactcttccc gccccggacg attcaggtca cggccgccgg
     1861 cgacacgacc gtcaacctgt catgggatcc ggggctcgac acccaccgga tcaccggcta
     1921 caaggtctac tgggacaccg attcgggagg ggcgaccccg tacgccttca actcggcggc
     1981 caaccccggg caggcggcga tcgccggcac cacggcgatc ctttcaggat tgactcccgg
     2041 cacgccgtac tacttcacgg tcacggccct gacctcgttc accagcccgt cgagcgggct
     2101 gacgacgagc tacgagagcc cgctctatcc gacgcaggtc tccggcgatc cggacttcgt
     2161 ctacccggtc gaggtgacgg ccacgacgac cggcggggtc tgcctgccgg tgtcgcaggt
     2221 cgcgaacctc caggtcagcc acgccgccgg cggtgacatc accctctgct gggatccgct
     2281 caacgatccc tgtctcgccg gctatcggat tctcggagcg acgacgcccg agtcggccgc
     2341 caactacacg accgtcgccg acaccgacct cggcacctgc tggaccggca atccttcgca
     2401 gcggttcttc ctcgttgtgg ctcgcggcac cggcggcacc gggccgtggg gccactacgg
     2461 ccagtagggg cccgcccgct gcgccggatc ggccgactcc ttctgtccct cgccctcctg
     2521 gtggggggcg gagcgttccc ggcgcgggcc cagtcggtca ccggctcgct caccggcacg
     2581 gtgcgcgatg ccgcgggggc tccactcgcc ggcgccacgg tgatcgcccg ctccctcgag
     2641 accggggccg agcgctcgat cctctccgat cgcgaggggc gttaccgctt cgagctcctc
     2701 accccgggct cctggaccgt ctcggcgcgt ctcggggggg atcctcccgg ccagaccatc
     2761 gtcgcgtcca tcgtcctgca g
//
LOCUS       DASRIQ010000328         7192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3250734, whole genome shotgun sequence.
ACCESSION   DASRIQ010000328 DASRIQ010000000
VERSION     DASRIQ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3250734"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="VFD06_07840"
     CDS             <1..1757
                     /locus_tag="VFD06_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZN03488.1"
                     /translation="DVMAGFPWEVLAPKIVGVRLSGSPSGWTAPKDVILKVLGILTVT
                     GGTNRVIEYFGPGTRALSCTGKGTITNMGAELGATTSVFPFDDRMEAYLRATDRAALA
                     DLAQKNRSLLGPDPEVEADPERFYEQVIEIDLSALEPQLVGPHTPDLARPISEVGKAV
                     RDHDYPDVIAVALIGSCTNSSYEDLGRAADVARQAAAHGARMKVPLLVTPGSEQIFAT
                     IKRDGQMQALEQVGASVLANACGPCIGQWRREEIKKGERNTIVTSFNRNFPGRNDANP
                     ETLAFMGSPEIVMAYGLAGRLSFNPLTDTLQGADGPWRLEPPAPAPDLPERGFVLSRQ
                     GYEPPAEAGAKVQVKVDPGSERLQILDPFDRNSDNDFVKMPVLIKTKGKTTTDHISPA
                     GLWLRFRGHLDRISDNMFIGAVNAFTGARGRTLQQLTGERDVEVPKVARAYRAAGRRW
                     VVVGDDNYGEGSSREHAAMSPRYLGAAAILARSFARIHESNLKKQGVLPLTFADPADY
                     DRVREDDRITLTNLRDLSPGTTVTAILHHADGTVDPIHLRHTLNQEQIAWFWAGSALN
                     LLAGQKGSGAESSTPPRA"
     gene            complement(1807..2388)
                     /locus_tag="VFD06_07845"
     CDS             complement(1807..2388)
                     /locus_tag="VFD06_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03489.1"
                     /translation="MTSGRKAGWALAGALVLAAGLGFGLAAPADGKWIHIRVVDRDDR
                     QTNVKVNMPVSALITMADSIEDEHFSGGHARIGHEGIDAAKLREVWQSLRNAQDMDFI
                     TVESNDEVVKVAKSGRFMIARVRGQRDGQGADAEVRLPLEVVDALLDAPEGRFNIKSA
                     LEALASQHSGELVTVTDGASRVRIWIDDRADAE"
     gene            2552..3373
                     /gene="mutM"
                     /locus_tag="VFD06_07850"
     CDS             2552..3373
                     /gene="mutM"
                     /locus_tag="VFD06_07850"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZN03490.1"
                     /translation="MPELPEVETVRRGLVPLLVGRRVTAVEVRDGRLRRPVTRKGLGP
                     LRGARLLAVRRRSKYLLLDTDAGSTLLLHLGMTGTLRIDDAGRPEAPHTHVVFRLDDG
                     RELRFSDPRRFGMVELIPNERLERHPRLAGLGPEPLETTLTGALLHAATRGRKKPVKN
                     LLMDTRTIAGIGNIYACEALFRAGLHPRRRVGRIDAAGWDRLVESLRAVLHEALGAGG
                     TTRRNFLNAEGEPGYFAVSLRVYDRQGKPCRRCGTTIRRIVQAGRSTFFCPGCQR"
     gene            complement(3416..3943)
                     /locus_tag="VFD06_07855"
     CDS             complement(3416..3943)
                     /locus_tag="VFD06_07855"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HZN03491.1"
                     /translation="MNDAKNAVRIGTAVLVLAFASCGRSPKAPESQPKAVNAPPDSSF
                     GEKPPAADPAGGGPSNPAGVEWLLVRIDGNDVPPGPRGAQARIRLDPATSEAAGLAVC
                     NQFKGRFDLDEAGLRFGPLATTRKACRGEAMDLEAKYLKGLQDTSRWSMNGDRLILSD
                     AAGRPLLEFAAHAGD"
     gene            complement(3940..4527)
                     /locus_tag="VFD06_07860"
     CDS             complement(3940..4527)
                     /locus_tag="VFD06_07860"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZN03492.1"
                     /translation="MVLLSRITTKTGDRGTTALGDGRRLRKDHPRIEATGAVDELNAV
                     LGLAIASDPSSDSAALLRGVQNDLFDLGADLTLPPGRGAARRRAPLRLTAAHVRPIEE
                     AIERANAALTPLRTFVLPGGSAAAAWLHLARTVCRRAERRLVTLSRREDLNAQAIVYL
                     NRLSDLLFVLARRANRDGREDILWVPGRRLEGDGS"
     gene            complement(4579..5283)
                     /locus_tag="VFD06_07865"
     CDS             complement(4579..5283)
                     /locus_tag="VFD06_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03493.1"
                     /translation="MDKAASLYREAALKFSCRETIHADEGKVLHYEYLYVIAADGTFH
                     DYRTRVGSSVEVSPRSIPAKHSLVRAYSWVFLFGRGFWRHVRYRILGTGQAMGRDAVL
                     VGFEPIPPLIKEINDWIGTAWVEPETGRIVRVEAEAADDLETLRAAEAVLAPAGVARR
                     RFLVRSVTTEFGVEKNGMRLPSQATVEIEEHLVPGRDGRPSSSRVKARTVQTYKDYRF
                     FGVTAEEKQPPAVSPP"
     gene            5569..6186
                     /locus_tag="VFD06_07870"
     CDS             5569..6186
                     /locus_tag="VFD06_07870"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN03494.1"
                     /translation="MRNVAARSDTMARVRAGRPADRRLEILRGAAAAFRDRGYAATSV
                     RDIARRLGMTPGNLYYYFRNKQEILYFCQDASLEVLLREARRIAASGAGPEEKVRALV
                     RAQVRCMLDELHGSAGHIEFHAIPRHRLKRVIARRDAYERLVRAAVAEGVRARRFAPC
                     DPKLATLAILGAVNWTARWYRPGGEWAPEAIADGFADLLVRGLLR"
     gene            6183..6761
                     /locus_tag="VFD06_07875"
     CDS             6183..6761
                     /locus_tag="VFD06_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN03495.1"
                     /translation="MSASEPRRPARRRAARPPAEEERTIPLRLLVNGERLEVSAPPHH
                     TLLEVLREEMGLTGTKHGCELGECGTCTVLVDGRPVLSCLVLPRACQGKAIVTVEGLQ
                     KGAEPDPLQRTFSELGAAQCGYCTPGILCAARALLDRTPDPTRDEIREALSGNLCRCT
                     GYIKIYEAVELAARRRTDPHARPSEETLYGRR"
     gene            6748..>7192
                     /locus_tag="VFD06_07880"
     CDS             6748..>7192
                     /locus_tag="VFD06_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03496.1"
                     /translation="MAGADSGGPAKPGGAGNGGAPRAGVVGAPVTKVDGPAKCAGQTK
                     FADDLVLPRMLFCKVHRAHVPHARIVNVDASKALAMPGVVAVMTGKDLPIPFGILPVS
                     QDEHALCPDKVRFVGDPVAAVAATSEDVAFDAMNAIAVEYEPLEPI"
BASE COUNT         1064 a         2550 c         2576 g         1002 t
ORIGIN      
        1 tggacgtgat ggccggtttc ccgtgggagg tgctggcccc gaagattgtc ggggtgcggc
       61 tctccggatc gccttcggga tggaccgccc ccaaggacgt catcctgaag gtgctcggca
      121 tcctgacggt cacggggggg acgaaccgcg tgatcgagta cttcggtccc gggacccgcg
      181 ccctctcctg caccggcaag gggacgatca ccaacatggg ggcggagttg ggggccacga
      241 cctcggtgtt cccgttcgac gaccggatgg aggcgtacct gcgtgccacc gaccgggcgg
      301 cgctcgcgga cctggcgcag aagaaccgct ccctcctcgg gccggatccg gaggtggagg
      361 cggacccgga gcggttctac gagcaggtga tcgagatcga cctgtccgcc ctcgagcccc
      421 agctcgtcgg cccgcatacc cccgacctcg cgcgcccgat ctccgaagtc ggaaaggcgg
      481 tgcgcgatca cgactatccc gacgtgatcg cggtggcgct catcgggtcg tgcaccaatt
      541 cctcctacga ggacctgggg cgcgcggcgg acgtcgcgcg ccaggcggcg gcgcacggcg
      601 cccgcatgaa agtgccgctc ctcgtcacgc ccggcagcga gcagattttc gccaccatca
      661 agcgcgacgg gcagatgcag gcgctcgagc aggtcggggc gagcgtgctg gcgaacgcct
      721 gcggtccgtg catcggccag tggcgccgcg aggagatcaa gaagggggag cggaacacca
      781 tcgtcacctc cttcaaccgc aacttcccgg ggcgcaacga cgccaacccg gagacgctcg
      841 ccttcatggg cagcccggag atcgtcatgg cgtacgggct cgcgggacgg ctctcgttca
      901 acccgctcac cgacacgctc cagggcgcgg acggcccgtg gcgcctcgag ccccccgcgc
      961 cggcccccga ccttccggag cgcggcttcg tgctgtcgcg gcaggggtac gagccgcccg
     1021 ccgaagccgg cgcgaaggtc caggtgaagg tggatccggg aagcgagcga ctgcagatcc
     1081 tcgacccctt cgatcgcaac agcgacaacg acttcgtgaa gatgccggtc ctcatcaaga
     1141 cgaaggggaa gacgacgacc gaccacatct cgccggcggg gctgtggctg cgtttccgcg
     1201 ggcacctcga ccggatcagc gacaacatgt tcatcggcgc ggtgaacgcc ttcaccggcg
     1261 cgcgcggccg gacgctgcag cagctgaccg gggagcggga cgtggaggtg ccgaaggtcg
     1321 cgcgggcgta ccgggcggcg ggacgccgct gggtcgtggt gggggacgac aactacggcg
     1381 aggggtcgag ccgggagcac gccgccatgt cgccgcgtta tctcggggcg gcggcgatcc
     1441 tcgcccgctc cttcgcccgc atccacgaga gcaatctcaa gaagcagggg gtgctgccgc
     1501 tgaccttcgc ggaccccgcc gactacgacc gggtgcgcga ggacgatcgg atcaccctca
     1561 cgaacctgcg ggatctctcg ccggggacga ccgtcaccgc catcctgcac cacgcggacg
     1621 gcacggtcga cccgatccac ctgcgccaca ccctgaacca ggagcagatc gcctggttct
     1681 gggcggggtc ggcgctcaac ctcctggcgg gccagaaagg aagcggggcg gagtcatcga
     1741 ccccgccccg cgcctgaccc gccgtccggg acgccgccga ggaggttcgg cgcccgcgag
     1801 ggcggattat tccgcgtcgg cccggtcgtc gatccagatc ctcacgcggc tcgctccgtc
     1861 ggtgacggtg accagctcgc cggagtgctg cgacgccagc gcttcgagcg ccgacttgat
     1921 gttgaaccga ccttccggcg cgtcgagcag ggcgtccacc acctcgagcg gcaggcggac
     1981 ctcggcgtcc gccccctggc cgtctcgctg gccgcggacg cgcgcgatca tgaaacggcc
     2041 cgacttcgcc accttcacga cctcgtcgtt cgactccacg gtgatgaagt ccatgtcctg
     2101 cgcattgcgc agcgattgcc agacttcccg gagcttcgcg gcgtcgattc cctcgtggcc
     2161 gatgcgcgcg tggccgccgc tgaagtgctc gtcctcgatc gagtccgcca tggtgatcag
     2221 ggccgacacc ggcatgttga ccttcacgtt ggtctgccga tcgtcgcggt ccaccacgcg
     2281 gatgtgaatc cacttgccgt cggccggggc cgccagaccg aatccgagcc ctgcggcgag
     2341 gaccagcgcc cccgccaacg cccatcccgc cttccgcccg ctcgtcattt ccatgccctc
     2401 cgtcaagcca atgtaacgcc ggcggccctg caaagttccc gggatcgagc ccgaccgcgg
     2461 ggggcggtcg ccggccgcac gacgttcagc gcggggatgg cggggcaggc ggtttgcgcc
     2521 cccggaggtg cggatggcct agactcaggg catgcctgag ctgccggaag tcgagacggt
     2581 gagacgtggt ctggtgcctc tgctggtcgg acgacgtgtc acggcggtgg aggtgcggga
     2641 cggccggctg cggcggccgg tgacgaggaa ggggctgggg ccgctccgcg gggcgcgact
     2701 cctcgcggtg cggcggcgca gcaagtacct cctgctcgac accgacgccg ggagcaccct
     2761 gctcctgcat cttgggatga ccggcacgct gaggatcgac gacgccggac ggccggaagc
     2821 gccgcacacg cacgtcgtct tccgtctcga cgacgggcgc gagctgcggt tctcggaccc
     2881 gcggcgcttc ggcatggtgg agctgattcc gaacgagcgg ctcgagcggc acccgcgcct
     2941 cgcggggctc gggccggagc cgctcgagac cacgctcacc ggggcgctcc tgcacgcggc
     3001 gacgcgcggc cggaaaaagc cagtgaagaa cctcctgatg gacacgcgaa ccatcgccgg
     3061 catcggcaac atctacgcct gcgaggcgct cttccgggcc gggctgcatc cgcgccggag
     3121 ggtgggccgc atcgacgcgg ccggctggga tcggctcgtg gagtcgctcc gagccgtgct
     3181 ccacgaagcg ctcggcgcgg gcggcacgac gcggcgcaac ttcctcaacg ccgaagggga
     3241 gccgggatac ttcgccgtgt cgctgcgcgt ctacgatcgg caggggaagc catgccggcg
     3301 ctgcggcacg accatccggc gcatcgtcca ggcggggcgg agcaccttct tctgtcccgg
     3361 ctgccagcgc tgagccggcc cctgcggaag agaggattcc gccgtgcggc ggcggtcagt
     3421 cccccgcgtg cgccgcgaac tcgaggagcg gccggccggc cgcgtcggag agaatcagcc
     3481 gatcgccgtt catgctccag cgcgaggtgt cctggagccc cttcagatac ttcgcttcga
     3541 gatccatcgc ctcgccgcgg cacgcctttc gcgtggtggc cagaggtccg aaccgcagcc
     3601 cggcctcgtc gagatcgaag cgccccttga actggttgca gacggcgagc cccgcggctt
     3661 cggacgtggc cgggtcgagc cggatccgcg cctgcgcccc cctcggcccg gggggcacgt
     3721 cgttgccgtc gatcctgacg agcagccact ccacgccggc cgggttggaa gggccgcccc
     3781 ccgccggatc tgcggccgga ggcttctcgc cgaacgagga gtctggaggg gcgttgacgg
     3841 ccttcggctg gctttccggg gccttgggac tgcggccgca cgaggcgaaa gccaggacca
     3901 ggacggcggt gccgattcga acagcgtttt tcgcgtcgtt cacgacccat ctccctcgag
     3961 gcggcggccg ggcacccaca ggatgtcctc gcggccgtcg cggttggcgc gacgggcgag
     4021 gacgaacagc aggtccgaca gccggttgag atagacgatg gcctgcgcat tcaggtcctc
     4081 gcggcgcgag agcgtgacca ggcgccgctc cgcgcggcgg cacacggtgc gcgccaggtg
     4141 cagccaggcg gcggccgcgc tgcctcccgg aaggacgaag gtgcgcagcg gagtgagggc
     4201 ggcgttggcc cgctcgatcg cctcctcgat cgggcggacg tgggccgcgg tcaggcgcag
     4261 cggcgccctc cgccgtgccg ccccgcgacc gggggggagg gtgagatcgg cgccgaggtc
     4321 gaacagatcg ttctggacgc cgcgcagcag cgcggccgag tcggaggacg gatcggaggc
     4381 gatcgccagc ccgaggaccg cgttcagctc gtccaccgcc ccggtcgcct cgatccgcgg
     4441 gtggtccttg cgcagccggc ggccgtcccc gagggcggtc gtgccgcgat cgccggtctt
     4501 cgtggtgatt cgggacagaa ggaccacgct gcgcctccca cggcgcgcct cgcgcgccgg
     4561 cgcacgatgg tacccgaatt acggcgggct caccgctggg ggctgcttct cctccgccgt
     4621 gactccgaag aaccgataat ccttgtaggt ctgcaccgtc cgcgctttga cgcgcgacga
     4681 ggatggccgc ccgtcgcggc cgggaacgag gtgctcctcg atctcgacgg tcgcctggct
     4741 cggcagccgc atcccgttct tctcgactcc gaactccgtc gtgacgctcc ggacgaggaa
     4801 gcgccgcctg gccacgccgg ccggcgccag gaccgcttcg gccgcccgga gggtttcgag
     4861 atcgtccgcc gcttccgcct ccacccggac gattcgtccc gtctccggct ccacccaggc
     4921 ggtgccgatc cagtcgttga tctctttgat gagcggcggg atcggctcga acccgaccag
     4981 aaccgcgtca cggcccatcg cctgcccggt cccgaggatc cggtagcgca cgtgccgcca
     5041 gaacccacgt ccgaacagga agacccacga gtaggcccgc acgagcgaat gcttcgccgg
     5101 gatcgaccgg ggcgagactt cgacggaact cccgacccgc gtccggtagt cgtggaacgt
     5161 cccgtccgcc gcgatgacgt agagatactc gtaatgcagc accttcccct cgtcggcgtg
     5221 aatcgtctcc cggcaggaga acttgagggc cgcctctctg tagagtgacg cggccttgtc
     5281 cattcgatcg aggagggagg gcagacgcac gtcggtgagc gcggaatcga ggcgggcggg
     5341 accggattcg acctccgccg ggaggacacg cgccggagcg agcgcgagca gcgcggcgac
     5401 cgcaagcgtc cgggaccgcc caaaacccca gccgcgcctg gcgacaaaag gctgcacgcg
     5461 gacatggtaa cggagaaggg gcggcgccgg agagccggcg gtcttgacag cgccggccgg
     5521 cgcgggtagt tttgaacgcg tgttcaaatc gggagaggcg cacgagtgat gcgaaacgtc
     5581 gcggccaggt cggacaccat ggcccgggtc agggccgggc gtcccgccga tcggcggctc
     5641 gagatcctgc gcggcgcggc ggccgccttc cgggatcgcg gctacgcggc gacgagcgtg
     5701 cgggacatcg cgcggcgcct cgggatgaca cccggcaacc tctattacta cttccgcaac
     5761 aagcaggaga tcctctactt ctgccaggac gcctcgctgg aggttctgct ccgtgaggcg
     5821 cggcgcatcg cggcctccgg agcggggccg gaggagaagg tccgcgccct cgtgcgggcg
     5881 caggtgcggt gcatgctgga cgagctgcac ggatcggccg gtcacatcga gttccacgcc
     5941 atcccgcgtc accgcctcaa gcgggtgatc gcgcggcgcg acgcctacga gcggctggtg
     6001 cgcgccgcgg tcgcggaggg ggtgcgcgcg cgccgcttcg ctccctgcga cccgaagctc
     6061 gccaccctcg ccatcctggg tgcggtcaac tggaccgcgc gctggtaccg gcccggaggg
     6121 gagtgggccc ccgaggcgat cgccgacggg ttcgcggacc tgctcgtgcg ggggctcctg
     6181 cgatgagcgc ttcggaaccc cggcggccgg cgcgacgccg cgccgcgcgc ccccccgcgg
     6241 aggaggagcg gacgatcccg cttcggctcc tcgtcaacgg cgaacgcctg gaggtctcgg
     6301 ccccgccgca ccacaccctc ctcgaggtgc tgcgcgagga gatgggtctc accggcacca
     6361 agcacggctg cgagctcgga gagtgcggca cctgcacggt cctcgtcgac gggcgtccgg
     6421 tactgtcgtg cctcgtcctg ccgcgggcct gccaggggaa ggcgatcgtc accgtcgagg
     6481 ggttgcagaa gggggcggag ccggatcctc tgcaacggac gttctccgag ttgggggcgg
     6541 cccagtgcgg ctactgtacg cccggcatcc tgtgcgcggc gcgcgccctt ctcgaccgca
     6601 ctcccgaccc gacgcgcgac gagatccgcg aagcgctctc cggaaacctc tgccgctgta
     6661 ctggctacat caagatctac gaggcggtcg agctggccgc ccgccgccgc accgacccgc
     6721 acgcgcggcc gtccgaggag acgctctatg gccggcgctg acagtggggg gccggcgaag
     6781 ccgggcggcg ccggaaacgg cggcgcgccc cgggccggcg tggtcggcgc ccccgtcacc
     6841 aaggtggacg ggcccgccaa gtgcgccggg cagacgaagt tcgccgacga tctcgtcctg
     6901 ccgcgcatgc tcttctgcaa agtgcaccgc gcgcacgtcc cgcacgcccg catcgtgaac
     6961 gtggacgcct cgaaggcgct cgcgatgccg ggagtggtcg cggttatgac cgggaaggac
     7021 ctgccgatcc cgttcggcat cctgccggtg agccaggacg agcacgccct ctgccccgac
     7081 aaggtccgct tcgtcgggga tcccgtggcg gcggtcgcgg cgacgagcga agacgtcgcg
     7141 ttcgacgcca tgaacgccat cgcggtcgag tacgagccgc tcgagccgat cc
//
LOCUS       DASRIQ010000329         6720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2998020, whole genome shotgun sequence.
ACCESSION   DASRIQ010000329 DASRIQ010000000
VERSION     DASRIQ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2998020"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..796)
                     /gene="truA"
                     /locus_tag="VFD06_07885"
     CDS             complement(38..796)
                     /gene="truA"
                     /locus_tag="VFD06_07885"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZN03497.1"
                     /translation="MRRAVTIKMTIAYVGTRYAGWQIQPGRPTIQGVLEEQLSRLLQE
                     PITLLGAGRTDSGVHAMGQVASLETTRHVPLDGLRRGMNARLPEDIRILEAAAATDGF
                     HARSDARGKEYAYRFSRGEVTSPFRAPFVCPVRGGLDLEAMRRAAPGFEGRHDFTSLA
                     PASCELEDRVRTITLARIEEEREEVVVRIRGDGFLRHMVRTIAGTLLDIGRGRREASD
                     VPGILAARDRRAAGTCAPARGLTLVKVFYAGADA"
     gene            911..2392
                     /locus_tag="VFD06_07890"
     CDS             911..2392
                     /locus_tag="VFD06_07890"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HZN03498.1"
                     /translation="MKVFRPFFRPVILALALAATAAGAAGPPDWKTPGKDWAKGPVRW
                     LMTEEEEKQFKALKTDEERAAFAKTFWEKRDPTPGTPDNEYEIIFWQRVEQADKAYKT
                     MLKEGSVSDFGHVFILLGPPTNMRKDSRYTYWLYEPSPVNGITQKMELSFAPIDTGYL
                     LRDKKTLEEYVGAHAEARGIGWKIPNLAPDASQEVAQAAPKEVVEDTSPESQRQNPIL
                     DAVLAKGSGPTDVPFQVTNDFYATADGTTLVVTTVEVPREAAHGGSTDALLPFARIAP
                     EGEGKPVNLTGALPFVPAPVADGPAAGFVYQARRNLKPGTYKQVVVVEDKVMKGTMGT
                     SVASIKVPDYSAKQFDMSSVALLAQYGKKEQSVGPDDAATGGAGLYSIGSFRLVPRAT
                     PVLTKADALAFYYQVYNPSPDTTGKPNLESSYTFYVKDASGWKPFRKPVVKQVGQVEL
                     WELPIASLLGANMVLPIDFRMEAKVTDKTNGQSLTREIQFSVR"
     gene            complement(2417..3367)
                     /locus_tag="VFD06_07895"
     CDS             complement(2417..3367)
                     /locus_tag="VFD06_07895"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HZN03499.1"
                     /translation="MSAVHRSRRRARRGEGAPDPLLRPGDRIAVVAPAGPVPIADLER
                     GIAVLQRRGYEVARGAHLADREAYLAGDDDARAADLNAAIRDRKVRLIWCARGGYGTP
                     RILERVDARALERDPKPLLGYSDETALQEVWRRAGAPILYGPHVVELGDRRAYHARSL
                     WGALAGEPVEVPIASARVVRQGRAEGLLLGGCLSLLVGLAGTPYEPPVDGAILFWEDV
                     NEQPFRLDRMLTHLKNAGWLRRIAGMVVGRLHGCVADDARLHRSIGEILDDRLEGTRI
                     PVAYGLPAGHTPGKWTLPLGGTARLDTRAGRLRIDPPAAR"
     gene            complement(3364..4518)
                     /locus_tag="VFD06_07900"
     CDS             complement(3364..4518)
                     /locus_tag="VFD06_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN03500.1"
                     /translation="MERLSRRLFDLCELSEQEHASSGLLKAFLRRHGFRVMSGAGGLP
                     TAFVARRRVGRGGPRIGLLAEYDALPDIGHACGHNLIGAAACGAAALSAHLLDGRGAE
                     IIVYGTPAEETIGGKIVLARRGLFRGLDAALMFHPSTEDRVYMTSLACHSFEVEFRGR
                     SAHAVASPEKGINALAALLALFAAMERLRPSLPPETRMPGIIADGGRRPNIVPDRAVG
                     RFTLRAGDLRRLGRVERAFFAAGRAAARNVGARVRFRPVDLPYAEMRTNGPLAEIFKE
                     ELRALGRGTVDTPRRGMGSLDMGNVSQIVPSIHPFVAIAPKSAPLHSRPFARRAGGPA
                     GRKGLEIASRALAATLLRAVHDPAILRAARRAFAAGGHPSGSPRSGGGAR"
     gene            complement(4578..4995)
                     /gene="rnpB"
                     /locus_tag="VFD06_07905"
     ncRNA           complement(4578..4995)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFD06_07905"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5245..6585
                     /gene="dnaB"
                     /locus_tag="VFD06_07910"
     CDS             5245..6585
                     /gene="dnaB"
                     /locus_tag="VFD06_07910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZN03501.1"
                     /translation="MATDITLEKEVPHSLDAERSVLGAILVENSSLNRAQEILKDSDF
                     YREPHRRLFKAMTALSERSAAIDPVTIKEELQRLGDLEVVGGQAYIASLLDGVPHSSN
                     IEHYARIVKEKAMLRSLIEAGGRIVQTAYEGSQEVEEILDQSERLVFQIAQDQLRAGF
                     VSMRVIADQGLKTIERLQQHRELVTGLSTGYATLDEMTSGFQPTDLIIIAARPGMGKT
                     SFALNIAQNCSRQGRKVGVFSLEMSREQLFLRLLTGQARVDAHAMRTGRLRREEWRRV
                     TDTLAEIAEVPISIDDTAGITVLEMRAKSRRLKMEQGLDLLIIDYLQLMRSRGRFENR
                     NQEISEISRSLKELAKELQVPVVAISQLSRAPEQRGNDRRPQLSDLRESGAIEQDADM
                     VMFIFREEVYKPTEENRGLAQVIIAKQRNGPIGTIEFAFLREYTRFEQREWRPE"
BASE COUNT         1028 a         2420 c         2350 g          922 t
ORIGIN      
        1 ccgccgacgt agccggcgcc gcccgtcacc aggaggttca cgcgtcggcc cccgcgtaga
       61 acaccttcac gagcgtcagc ccacgcgccg gagcgcaggt gccggcggcc cggcgatccc
      121 gcgccgctag gatccccggc acgtcggagg cttcgcgccg tccgcgtccg atgtcgagca
      181 gcgttccggc gatggtccgc accatgtgcc ggagaaagcc gtccccccga atccgcacga
      241 cgacctcctc cctctcctcc tcgatgcgcg ccagggtgat ggtccgcacc cggtcctcca
      301 gctcgcacga ggcgggggcc agcgaggtga agtcgtggcg cccctcgaag cccggtgcgg
      361 cgcgtcgcat cgcctcgaga tcgagaccgc cccgcaccgg gcagacgaac ggggcgcgga
      421 acggggaggt cacctcgccg cggctgaaac ggtaggcgta ttccttgccg cgcgcgtcgc
      481 tgcgggcgtg gaagccgtcc gtcgccgccg cagcctcgag gatccggatg tcctccggga
      541 gacgcgcgtt catcccgcgc cgcagcccgt cgagcggcac gtggcgcgtg gtctccaagc
      601 tcgcgacctg ccccatcgcg tgcacccccg agtcggtgcg gccggcgccg agcagggtga
      661 tcggctcctg cagcaggcga gacaattgtt cctcgaggac gccctggatc gtcggccgtc
      721 ccggctggat ctgccatccc gcgtagcgcg tgcccacgta cgcgatcgtc atcttgatcg
      781 tcactgcgcg gcgcattata gggggctcct cccccatcct tcttattacc tgccgtgcgg
      841 ggggccgcgc ggggccgggt ggagaggggg gcgggttgcc gctataattc cggcctctgt
      901 gaggagaccg atgaaagtgt ttcgtccgtt cttccgtccc gtgattcttg cgctggcgct
      961 ggcggcgacc gccgcggggg cggcggggcc tcccgattgg aagacacccg ggaaagactg
     1021 ggccaagggg ccggtccgct ggctgatgac cgaggaggag gagaagcagt tcaaggcgct
     1081 caagacggac gaggagcgcg ctgccttcgc caagacgttc tgggagaagc gcgacccgac
     1141 ccccgggacc cccgacaacg agtacgaaat catcttctgg cagcgggtcg agcaggcgga
     1201 caaggcctat aagacgatgc tgaaggaggg ctcggtctcc gacttcggcc acgtcttcat
     1261 cctgctcggg ccgccgacca acatgcgcaa ggactcgcgc tacacctact ggctctacga
     1321 gccgagcccg gtcaacggca tcacccagaa gatggagctc tccttcgcgc cgatcgacac
     1381 cggctacctg ctgcgcgaca agaagacgct cgaggagtac gtcggggcgc acgccgaggc
     1441 gcgcggcatc ggctggaaga ttccgaacct cgcgcccgac gcctcgcagg aggtcgcgca
     1501 ggcggcgccg aaggaggtgg tcgaggacac ctcccccgag agccagcggc agaacccgat
     1561 tctcgacgcc gtcctcgcca aagggagcgg gccgaccgac gtgccgttcc aggtgacgaa
     1621 cgacttttac gccaccgccg acggcaccac gctcgtggtc acgacggtcg aggtgccgcg
     1681 ggaggcggcg cacggcggca gcaccgacgc gctcctgccg ttcgcgcgca tcgcgccgga
     1741 gggggagggc aagccggtga acttgaccgg cgcgctgccg ttcgtccccg cccccgtcgc
     1801 agacggcccc gcggccggct tcgtctacca ggcccgccgc aacctcaagc cggggactta
     1861 caagcaggtg gtggtggtcg aggacaaggt gatgaaggga accatgggga cgagcgtcgc
     1921 ctcgatcaag gtccccgact acagcgccaa gcagttcgac atgtcgagcg tggcgctcct
     1981 ggcgcaatac ggcaagaagg agcagagcgt cgggccggac gacgccgcaa ccggcggcgc
     2041 cggcctgtac tccatcggat cgttccggct ggtgccccgc gccacgccgg tgctcaccaa
     2101 ggcggacgcg ctggccttct actaccaggt gtacaacccg tcccccgaca ccaccggcaa
     2161 gccgaacctg gaatcgagct acaccttcta cgtcaaggac gcctcgggct ggaagccgtt
     2221 ccgcaagccg gtggtgaagc aggtcggcca ggtggagctg tgggagctgc cgatcgccag
     2281 cctgctcggc gcgaacatgg tgcttccgat tgatttccgc atggaggcga aggtcacgga
     2341 caagacgaac ggccagtcgc tgacgcgcga gatccagttc agcgtccgct gagcggggca
     2401 cccggcccgg ggccgctcag cgggcggccg gcgggtcgat ccgcaatcgt ccggcgcgcg
     2461 tgtcgagccg cgccgttccc cccaggggca gggtccactt ccccggcgtg tgtcccgccg
     2521 gaaggccgta cgccacgggg atgcgggtcc cttccagccg gtcgtcgagg atctccccga
     2581 tcgagcggtg cagacgcgca tcgtccgcga cgcagccgtg caaccgtccg acgaccattc
     2641 ccgcgatccg ccgcagccag ccggcgtttt tcaggtgggt cagcatccgg tcgagccgga
     2701 acggctgctc gttgacatcc tcccagaaga ggatcgcccc gtcgaccggc ggctcgtacg
     2761 gcgtcccggc gaggccgacg agcagcgaca ggcagccacc caggagcaga ccttcggccc
     2821 gcccctgccg caccacccgg gcgctcgcga tcggaacctc gaccggctcc cccgcgagcg
     2881 cgccccacag cgaccgcgcg tggtacgccc gccggtctcc cagctccacc acgtgcggcc
     2941 cgtacaggat cggcgcgccg gcccggcgcc agacctcctg gagcgccgtc tcgtcgctgt
     3001 agccgagaag cggcttcggg tcgcgctcga gcgcccgcgc gtcgacgcgc tcgaggatcc
     3061 gcggggtgcc gtagccgccg cgcgcgcacc agatgagccg gaccttgcgg tcgcggatgg
     3121 cggcgttgag atcggcggcg cgggcgtcgt cgtctccggc gaggtaggcc tctcggtccg
     3181 ccaggtgcgc gccgcgcgcc acctcgtaac cgcgccgctg cagcacggcg atgccgcgct
     3241 cgaggtccgc aatcggcacc gggccggccg gggcgacgac ggcgatgcga tcccccggcc
     3301 tcaacagggg atcgggcgct ccttccccgc ggcgcgcgcg gcggcgggat cggtggactg
     3361 cgctcaccgc gcgccgccgc cggaacgcgg ggagccggac ggatggccgc cggcggcgaa
     3421 ggccctccgc gccgcccgga ggatcgccgg atcgtgcacc gcgcgcagga gcgtggcggc
     3481 gagcgcgcgc gacgcgatct cgagcccctt cctgccggcc gggcctcccg cgcggcgcgc
     3541 gaacggacgc gagtgcagcg gggcgctctt cggcgcgatc gccacgaacg gatggatcga
     3601 cgggacgatc tggctgacgt tccccatgtc gagcgagccc atgccgcgcc ggggcgtgtc
     3661 cacggtgccg cgcccgaggg cccgcagctc ttccttgaag atctcggcga gcggcccgtt
     3721 ggtgcgcatc tcggcgtacg gcaggtcgac cgggcggaag cgcacccggg cgccgacgtt
     3781 gcgcgccgcg gcccgtcccg ccgcgaagaa ggcccgctcg acccgcccca gccggcggag
     3841 gtcgcccgcg cggagcgtga agcgtccgac ggcgcggtcc gggacgatgt tcggccggcg
     3901 tcccccgtcc gcgatgatgc cgggcatccg cgtctccggc ggcaggctcg ggcgcagtcg
     3961 ctccatcgcg gcgaacagcg ccagaagcgc ggcgagggcg ttgatcccct tctccgggga
     4021 ggcgacggcg tgcgccgagc ggccgcggaa ctcgacctcg aacgagtggc acgcgagcga
     4081 ggtcatgtag acgcggtcct cggtggaggg atggaacatg agggcggcgt cgagcccccg
     4141 gaacagaccc cggcgggcca ggacgatctt gccgccgatc gtctcctccg ccggcgtgcc
     4201 gtagacgatg atctccgccc cccgtccgtc gagcaggtgg gctgagagcg ccgcggcgcc
     4261 gcaggccgcc gcgccgatga ggttgtggcc gcaggcgtgc ccgatgtccg gaagtgcgtc
     4321 gtactcggcc agcagcccga tgcgcggtcc gccgcggccg acccggcgcc gggccacgaa
     4381 ggcggtcggc aatcctccgg cgccgctcat gacgcggaag ccgtgccgcc gcaggaacgc
     4441 tttcaggaga ccgctcgagg cgtgctcctg ctccgacagc tcgcacagat cgaagagccg
     4501 gcgggacaga cgctccacct cgggggcgag acgccgccag gcgcgcgcga accgcggatc
     4561 ggcgttcgaa gagatctggc cggagtgaac cgtaagccga atcctgttgc cgccgtcgcc
     4621 ggcggccgat ggtcattcct ctacccgcgc ggtctcccgc gcgggcggcg gcacgacctc
     4681 gagttcatcg aggccccgtc gccaagcgac cgacccggga gctcgggcgg gccgcccgat
     4741 gcgctcccct atttggcctt gctccctgcg gggtttgccg agccgccccg gtcacccggg
     4801 gcgctggtgg gctcttaccc caccgtttca cccttaccgc gttcgcaggc gagcccgcga
     4861 acacggcggt ctgttttctg tggcactttc cttcccgtca ccgggactgg gtgttaccca
     4921 gcgcagcgcc ctgtggagtt cggactttcc tctccccgga cgcaaggccc gggcagcgac
     4981 catccagttc actccgacca aggcagatcg tagacgatcg gcgcgatgcc tcgcaatgcg
     5041 gggatcgcgg accgcggaga tcggggcgca aagcgtgttt ccccggcgtg gcacgcggcg
     5101 cgtgaactgt gtcctcgcgg cccggcgcgc cgcgtgccac gcgaacctgc tgcgtttcga
     5161 gcctgcatcc ccgcagatcg gagccgtctc cgcgggatcc attcggtgtt acgatgccgc
     5221 ccgccccgat cgagatcaat tccgatggcc accgatatca cgctggagaa ggaggttccg
     5281 cacagcctcg atgcggagcg atcggtgctc ggcgccatcc tggtggagaa cagcagcctc
     5341 aaccgggcgc aggagatcct caaagactcc gacttctacc gcgagccgca ccggcggctg
     5401 ttcaaggcga tgaccgcgct gtcggagcgc tccgccgcca tcgacccggt cacgatcaag
     5461 gaggagctgc agcgcctcgg cgacctcgag gtggtgggcg gacaggcgta catcgcgtcg
     5521 ctgctcgacg gcgtgccgca ctcctcgaac atcgagcact acgcgcgcat cgtcaaggag
     5581 aaggcgatgc tgcgatccct catcgaggcc gggggccgca tcgtccagac cgcctacgag
     5641 ggatcgcagg aggtcgagga gatcctcgac cagtcggagc gcctggtgtt ccagatcgcc
     5701 caggaccagc tgcgcgccgg cttcgtgtcg atgcgggtga tcgccgacca ggggctcaag
     5761 acgatcgagc gcctgcagca gcaccgcgag ctggtcaccg ggctgtcgac cggctacgcc
     5821 acgctcgacg agatgacctc cggcttccag ccgaccgacc tcatcatcat cgccgcccgc
     5881 cccggcatgg ggaagacctc gttcgccctc aacatcgcgc agaactgctc gcggcagggg
     5941 cgcaaggtcg gcgtcttctc gctcgagatg tcgcgcgagc agctgttcct gcgtctgctg
     6001 accggccagg cgcgcgtgga cgcgcacgcc atgcgcaccg gccggctgcg gcgcgaggag
     6061 tggcggcggg tcaccgacac gctggcggag atcgccgagg tgccgatctc gatcgacgac
     6121 accgccggca tcaccgtcct ggagatgcgc gccaagagcc ggcggctgaa gatggagcag
     6181 ggactggatc tgctcatcat cgactacctg cagctgatgc gcagccgcgg ccgtttcgag
     6241 aaccgcaacc aggagatctc cgagatctca cgctcgctca aggagctcgc caaggagctg
     6301 caggtgccgg tggtcgccat ctcgcagctg tcgcgcgccc cggagcagcg cggcaacgac
     6361 cggcgcccgc agctttccga cctgcgcgag tcgggggcca tcgagcagga cgccgacatg
     6421 gtcatgttca tcttccgcga ggaagtgtac aagccgaccg aggagaaccg cggcctggcg
     6481 caggtgatca tcgccaagca gcgcaacggc cccatcggca ccatcgagtt cgccttcctg
     6541 cgcgagtaca cccggttcga gcagcgggag tggcgcccgg agtgagcggc ccgtcccccg
     6601 gccttccgcc ggaggcgggg cgggccacct ggctcgagat cgacctcacc gcgctggccc
     6661 acaacctcaa ggtcgtccgc gcccacctct ccggggcgcg cgtcatggcg gtggtcaagg
//
LOCUS       DASRIQ010000330         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3251603, whole genome shotgun sequence.
ACCESSION   DASRIQ010000330 DASRIQ010000000
VERSION     DASRIQ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3251603"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(439..>2031)
                     /locus_tag="VFD06_07915"
     CDS             complement(439..>2031)
                     /locus_tag="VFD06_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZN03502.1"
                     /translation="PGGAARAFDALAHSRALVLDEMAERHHALLERGSKSLAPLIESL
                     ARAREGLGAALQGVPDPARPEEFPAALERARAAVDDADRALAERSAPFRAARQEKNAG
                     LDEILHSLPPEAALVAYARYRDPGAGVRPGETSYLAFVLASSRRDPIPVALGQARTID
                     RLVADWRRTIERAPDDAAAADAYNEAGLGLRQAIWDPVAAHLGRAKIVFIVPDADLNL
                     VNYATFPAPGGRYLLETGPTLHYLSAERDLVAEAAGGREARGLLALGGPDFDAAGPAG
                     DSPATAYRSALPACARAASLRFDALPGAREEAREVASQVRRAAGRSGKEPILVLTGQE
                     AGEEAVKRLSHGRRILHLATHHFWFGDACDAAAPRADDAPAPPSAQRPLRASPLLLTG
                     LALAGVNRRDDPKRSADQEDGLLTADEISSLDLRGTEWVVLSACQSGVGPVLPGEGVL
                     GLRRAFRIAGARTLVMSLWAVGDQDAREWVRELYAARLGGRSTAESLRLASRRILDSR
                     REAGATTHPHTWGAFVSAGDWR"
BASE COUNT          288 a          736 c          730 g          277 t
ORIGIN      
        1 cgccgcggca cccagggcgg ccacgaagtt gcgccggttc gagcggacga ctctcatcga
       61 agctcctcct tccggaccag gggatgctac cccgatccgc cggaggattc cgctcatccg
      121 acttcgaagc gaggcctcac gggcaaggca gcgggttggc ccgttccgtg ccgctcgagg
      181 accggccctt ggttccctcc tcacgcagcc ggttcacgcc cgtgacgagg tagaacaggc
      241 cggtacccgt cgacggcaag gtgtcatcga ccgccgaggt ccccgcgacc tgagcgaacg
      301 gaaggatcca tgcgcggcgg ggggctccgg tgaagcgtct ttgcgcgcag ggtatcacgg
      361 cgtgtctcac gtcagggcgc aggctgccaa gtcacgtcgg tgctcgtggc ctcataccga
      421 ccgaatgccg atgaggaatc agcgccagtc accggcgctg acgaaggcgc cccaggtgtg
      481 gggatgggtc gtcgctccgg cctcccggcg ggaatcgaga atgcggcgtg aggcgaggcg
      541 gagcgattcg gccgtcgatc ggccgcccag gcgggcggcg tagagctcgc ggacccattc
      601 cctggcgtcc tgatcgccca ccgcccacag actcatgacg agggtgcggg ccccggcgat
      661 ccggaaggcg cgccgcaggc cgagcacgcc ttctccaggc agaaccggcc cgacgccgct
      721 ctggcaggcg gacagcacga cccattccgt tccccgcaga tccagcgagg agatctcgtc
      781 ggcggtgagc aggccgtctt cctgatcggc ggaacgcttc ggatcgtccc ggcggttgac
      841 ccctgccagc gcgaggccgg tcagaaggag aggactggcg cgcaggggcc gttgcgcgct
      901 cggcggtgcg ggcgcgtcgt ccgccctcgg tgccgccgca tcgcacgcgt cgccgaacca
      961 gaagtggtgg gtggcgaggt gcaggatccg ccgtccgtgc gacaggcgct tgaccgcctc
     1021 ctctccggct tcctgtcctg tgagaaccag gatcggctcc ttgccgctcc tgccggccgc
     1081 ccgccgcacc tgggaagcga cctcgcgcgc ctcctcgcgc gcgcccggca gcgcgtcgaa
     1141 gcgcaggctc gcggcccgcg cgcaggccgg cagggcgctc ctgtacgcag tcgcgggcga
     1201 atcgccggcg ggtcctgcgg cgtcgaagtc aggcccaccg agcgccagca gtcccctggc
     1261 ctcgcgcccg ccggcagcct ccgcaaccag gtcgcgctcc gccgagaggt aatgcagcgt
     1321 cggtccggtc tcgagaagat aacgccctcc cggcgcgggg aatgtggcgt agttcaccag
     1381 gttcagatcg gcatcgggaa cgatgaagac gatcttcgcc cggccgagat gggctgcgac
     1441 ggggtcccag atggcctggc gtagccccag accggcctcg ttgtatgcgt ccgccgccgc
     1501 ggcgtcgtcc ggagcccgct cgatcgtccg ccgccagtcc gccaccagcc ggtcgatggt
     1561 tcgggcctgc cccagcgcca cggggatcgg atcccgtcgc gaagacgcca ggacgaacgc
     1621 caggtatgag gtttccccgg gccggacgcc cgcccccgga tcccgatagc gcgcgtacgc
     1681 gacgagcgcc gcttcgggcg gcagggagtg gaggatctcg tcgagaccgg cgttcttctc
     1741 ttgccgcgcg gcgcggaagg gagcgctccg ttccgccagc gcccggtcgg cgtcgtccac
     1801 ggcagcgcgg gccctttcga gcgcggcggg gaactcctcg ggacgggccg gatcgggaac
     1861 cccctgcagc gcggcgccca gcccctcccg cgccctggcg agcgactcga tcaggggggc
     1921 cagagacttg gagccgcgct ccagaagcgc gtggtgccgc tcggccatct cgtcgagcac
     1981 cagcgccctg gagtgcgcga gggcgtcgaa cgcccgggcc gcgccgccgg g
//
LOCUS       DASRIQ010000331         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2461030, whole genome shotgun sequence.
ACCESSION   DASRIQ010000331 DASRIQ010000000
VERSION     DASRIQ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461030"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3638
                     /locus_tag="VFD06_07920"
     CDS             <1..>3638
                     /locus_tag="VFD06_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN03503.1"
                     /translation="DIDNDGDNTGVGEGDIIPNNGAVDRGILNSTTVADPLHRIPFDF
                     DRNSGNWLGLRHPDSKIGIGIGPLNMWEYTKTGACGAQTTIADGNATGLFQPPGAAGI
                     WHTGDTNPATPSGQICDEYPYPNDAATPTFEEVVFDILVSPIIAKVNQRNDIRGFPYV
                     VEFQRVGVNANIQTADYAGADIDFDSDVDRDQGNCLMCEYWYYRFGDIDTIVSFNSYN
                     YGINPASNTPQRTFGPLTDPNNSVATGTVTGDETGFTGFTPDTNPASDSPIPVAPPDF
                     RPFPSPGAPQVCVPGCVVPADCCEQNTVAGPERNMDIVMLDYEDGLIYLSLGLGQGEP
                     TGAFAPGPAGNRWQIGVGFWVNESTTNDTDYGLGIDDVVLEWDESHPIPEVGGAACTR
                     FGGINQPAGQQCATLNVDRLTIYECNESIGITVYDPRRAGTGAVTVFGVTDTDSNPVS
                     TGVVSAKHPRKSFSIAETSTPGLFKGNVVVGTLFDNTSLLFTNPGGDSNMTFYYLDPE
                     CDGDRDGSMGENSFANLDNDNIGAGDKCPFLYNPLQEDTDADGVGNLCDNCPGVANTN
                     QVDSDADGVGDACDFDDIDFDGVVNAVDNCPDVYNAAQIPGGGGSLKGDACSGNGDRD
                     GDGIADRLDNCVRTANPTQTDTDGDQVGNACDGDCGNPQQTTFTAGSCIRTNNTICTA
                     DGQCPVTGHCTVTPTVTCSTNANCSGAGNTCTGQAQETCQRQGVTNNGTCGTTEDDLD
                     ADGVTDAIDNCPVVSNPSSLAGAILQLDADSDGRGDACDPAQTVDDDNNGIPDDAITF
                     NSIIACRKLPLGTITVLANSVVDVGGDGDPFADAGETARMALVLRNDSGFNLTNATLV
                     LGTIDPDVACVTKSTILVPSLPDGATLDTATLGNPAGRFEYVISTTASTTNAANPAHA
                     DFFLSVVSNEVVGTTTPVPVGTLIDLDLPGGGLPPKVLGPDGLPNTSDDGLIVENFET
                     ERDGTAQISISNLPRGTPGVLNDTIGVWVGTAPGGINTLAGIGCAGFNVPPADPSCII
                     DPDNDMDWHIHCPAGTCPNSAGFITPAGGVMSHSGPNSLHWGHHFDPATRDGDSVKFR
                     QLAAFQTDAINLTPVPGVGDLELSFFHIADMMDNNSYNLPTGQANDFGDVHIQSFDPA
                     GGGGAGAWGFWDRLAPFQNVYDHTSYIWSAFGVSPTYCVLTPTDTGSGGFAPRGVKET
                     LCFPNGIWS"
BASE COUNT          666 a         1343 c         1147 g          482 t
ORIGIN      
        1 gacatcgaca acgacggcga caacaccggc gtgggagaag gggacatcat cccgaacaac
       61 ggggccgtcg atcgcggcat cctgaactcc acgacggtcg ccgatccgct tcacaggatt
      121 ccgttcgact tcgaccgcaa cagcggcaac tggctcggac tgcgccaccc ggacagcaag
      181 atcgggatcg gcatcggtcc gctcaacatg tgggagtaca cgaagaccgg cgcctgcggc
      241 gcgcagacga ccattgcgga cggcaacgcc accgggctct tccagccgcc gggggccgcc
      301 ggaatctggc acaccggcga caccaacccg gcgacgccgt cggggcagat ctgcgacgag
      361 tacccctatc cgaacgacgc ggcgacgccg acgttcgagg aggtggtgtt cgacatcctc
      421 gtgtcgccga tcatcgccaa ggtcaaccag aggaacgaca tccgcggctt cccctacgtc
      481 gtcgagttcc agcgggtggg cgtcaacgcc aacatccaga ccgcggacta cgccggcgcc
      541 gacatcgact tcgacagcga cgtcgatcgg gaccagggga actgcctgat gtgcgaatac
      601 tggtactacc ggttcggcga catcgacacg atcgtgtcgt tcaactccta caactacggc
      661 atcaacccgg cgagcaacac gccgcagcgc accttcggtc cgctcaccga cccgaacaac
      721 tcggtggcca ccggcacggt gaccggcgac gagaccggct tcaccggctt cacgccggac
      781 accaacccgg ccagcgatag cccgatcccg gtcgcgcctc ccgacttccg gccgttcccg
      841 tcgccgggcg cgccccaggt ctgcgtcccc ggttgcgtcg tcccggccga ctgctgcgag
      901 cagaacaccg tcgcggggcc ggagcgcaac atggacatcg tgatgctcga ctacgaggac
      961 ggtctcatct acctgagcct cggcctcggg cagggcgagc cgaccggggc gttcgccccc
     1021 gggccggcgg ggaaccgctg gcagatcggc gtcggcttct gggtcaacga atcgacgacc
     1081 aacgacaccg actacggcct cggcatcgac gacgtcgtcc tcgaatggga cgagtcgcac
     1141 ccgattccgg aggtcggcgg ggcggcctgc acccgcttcg gcggcatcaa ccagccggcg
     1201 gggcagcagt gcgccacgct caacgtggac cgcctcacga tctacgagtg caacgagtcg
     1261 atcgggatca ccgtctacga cccgcggcgc gccgggaccg gcgccgtcac ggtgttcggc
     1321 gtgaccgaca ccgactccaa cccggtcagc accggggtgg tctcggcgaa gcacccgcgc
     1381 aaatcgttca gcatcgcgga gacctccacg ccggggctgt tcaaggggaa cgtcgtcgtc
     1441 ggcacgctgt tcgacaacac gagcctgctg ttcacgaacc cgggcggcga cagcaacatg
     1501 acgttctact acctcgaccc ggagtgcgac ggcgaccgcg acggcagcat gggcgagaac
     1561 tcgttcgcga acctcgacaa cgacaacatc ggcgcgggag acaagtgccc gttcctctac
     1621 aacccgctcc aggaagacac ggacgcggac ggcgtgggca acctgtgcga caactgcccc
     1681 ggcgtcgcca acaccaacca ggtggattcg gacgccgacg gcgtcggcga cgcctgcgac
     1741 ttcgacgaca tcgacttcga cggcgtggtc aacgccgtcg acaactgccc ggacgtctac
     1801 aacgcggccc agatcccggg cggcggcgga tcgttgaagg gggatgcgtg ctccggcaac
     1861 ggcgaccgcg acggtgacgg catcgcggac cggctcgaca actgcgtccg cacggcgaac
     1921 ccgacgcaga ccgacaccga cggcgaccag gtcggcaacg cctgcgacgg cgactgcggc
     1981 aacccgcagc agacgacgtt cacggccggg agctgcatcc ggaccaacaa caccatctgc
     2041 accgccgacg gccagtgtcc ggtcaccggc cactgtaccg tgaccccgac cgtcacctgc
     2101 agcacgaacg ccaactgctc gggggcgggg aacacctgca ccggccaggc gcaggagacc
     2161 tgccagcgtc agggcgtcac gaacaacggc acctgcggga ccaccgagga cgacctggac
     2221 gccgacggcg tcaccgacgc catcgacaac tgcccggtcg tctccaaccc gtcgagcctg
     2281 gcgggcgcga ttctccaact cgacgccgac tccgacggcc gcggcgacgc ctgcgacccg
     2341 gcccagaccg tggacgacga caacaacggc atcccggacg acgcgatcac cttcaactcg
     2401 atcatcgcct gccgcaagct gccgctcggc accatcacgg tgctcgccaa ctcggtggtc
     2461 gacgtgggcg gcgacggcga tccgttcgcg gacgccggcg agaccgcccg catggcgctc
     2521 gtcctcagaa acgacagcgg gttcaacctc accaacgcga cgctggtgct cggcacgatc
     2581 gacccggacg tggcctgcgt gaccaagtcc accatcctcg ttccgtcgct cccggacggc
     2641 gcgacgctcg acacggcgac cctcggcaac ccggccggcc ggttcgagta cgtcatctcc
     2701 acgacggcgt cgaccaccaa cgccgccaac ccggcgcacg ccgacttctt cctgagcgtc
     2761 gtgtcgaacg aggtggtcgg gacgacgacg ccggttccgg tcggcacgct catcgatctc
     2821 gatctccccg gcggcggcct gccgcccaag gtgctcggcc ccgacggctt gccgaacacc
     2881 tcggacgacg gcctcatcgt ggagaacttc gagaccgagc gggacggcac ggcgcagatc
     2941 tcgatcagca acctgccgcg cggcacaccg ggcgtgctca acgacacgat cggcgtctgg
     3001 gtcggcaccg cccccggcgg catcaacacc ctcgccggga tcggctgcgc cgggttcaac
     3061 gtcccgccgg cggatccgtc gtgcatcatc gatccggaca acgacatgga ctggcacatc
     3121 cactgtccgg ccggcacctg cccgaactcc gcgggcttca tcacgccggc gggcggcgtc
     3181 atgtcccaca gcggcccgaa ctcgctgcac tggggccacc acttcgatcc ggccacgcgc
     3241 gacggcgaca gcgtcaagtt ccgccagctg gccgcattcc agaccgacgc gatcaacctg
     3301 accccggtcc ccggcgtcgg cgacctcgag ctgtcgttct tccacatcgc cgacatgatg
     3361 gacaacaact cctacaacct gccgacgggc caggcgaacg acttcggaga cgtgcacatc
     3421 cagtcgttcg atccggccgg cggcggcggg gcgggggcgt ggggcttctg ggatcggctg
     3481 gcgccgttcc agaacgtcta cgaccacacc tcctacatct ggtcggcgtt cggcgtgtcg
     3541 ccgacgtact gcgtcctgac cccgaccgac accggcagcg gcggtttcgc gccgcgcggt
     3601 gtcaaggaga cgctgtgctt cccgaacggg atctggtc
//
LOCUS       DASRIQ010000332         5505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1953038, whole genome shotgun sequence.
ACCESSION   DASRIQ010000332 DASRIQ010000000
VERSION     DASRIQ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953038"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFD06_07925"
     CDS             complement(<1..193)
                     /locus_tag="VFD06_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZN03504.1"
                     /translation="MHQYSVRDVEKLAGLPRRRIRSLVEAGLVSPARGPRNTWLFSFQ
                     DLTVLRSARALATARVPRQR"
     gene            complement(215..1126)
                     /locus_tag="VFD06_07930"
     CDS             complement(215..1126)
                     /locus_tag="VFD06_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZN03505.1"
                     /translation="MAPRAIASLTVSFGLVSIPVKLFSATEASHAISFNLLHKGCGSR
                     LKQQYLCAKDEVPVPRDEMVKGYEFAKDQYVMFSPEELKAMEEAGTHTADISEFVPIA
                     SIDPVYFDKAYYLAPDKGGNKPYALLARALQQSGKCAIGRWAARGKQYIVMIRPVNGG
                     LVMQQLLYAGEVRSLEDLEIPPGDVKDAELKLAQQLIEQQASDTFDPAPYKDEVRTRI
                     EAAVQKKVEGQEITMAEVPEGGGQVIDLMEALRASLERKAPGKAREPESAARETQDAG
                     REAQAGGRKGLRRAQDAETRPKKTAKK"
     gene            complement(1143..3944)
                     /gene="ligD"
                     /locus_tag="VFD06_07935"
     CDS             complement(1143..3944)
                     /gene="ligD"
                     /locus_tag="VFD06_07935"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HZN03506.1"
                     /translation="MPRPPKAKPPQRPPKSSAPPPKSAAPKPPASPSLDEYAAKRAFN
                     ATPEPPPAMAEREGPLLFVIQQHAARAMHYDLRLEFDGVLKSWPIPKGPSLDPGEKRL
                     AVRTEDHPYDYASFEGIIPPGQYGAGEVIVWDCGVWSPDEGGTWFHDRAEAEKRAREG
                     IEKGKLGFTLRGEKVKGSWALVRTKDAKTWLLIKHRDRFVSKEDLTARDRSVLSGATV
                     EEMKVRPAHRIPASRLVPAGKVEPMPAALEPMHAEIGPAPFNHPDWMWEPKLDGYRAL
                     AFLDGAKVRLRSRRGHDLGEAFPRLIEELGQQAAGTMILDGEIVALGLDGRPSFNALQ
                     NRVQAKTPAEIAAAERDVSVVFYCFDLLFFAGVDLRKHPYRDRRRYLAQCLLPTPRVR
                     PVHATEDGVALNQASLASGFEGVVGKRKDSRYESGRRTATWLKIKPTASADFVVGGYT
                     KGKGSRSDLGAILVGYWDKKKLIFASHVGSGLDERTVTALKKRLEPLRRDACPFAEKP
                     DLPNPTTWVEPEIVVEVKFHSWTEDDHLRAPVFIRIRDDLRPRDVRRVAVSAPATAAP
                     GKEEAGQAGDAGGGTREILAQLDAARKDFPLVVGPHRIRLTHLDRVYWPADPALQQPP
                     LTKRDLLRYLAQVSPYMLPHLADRPLTMIRMPDGIQGHRFFQKHWEQERPEFVETITV
                     FSSHKEESHEYLMCSNLPTLLWLAQLGTLEFHVWHSRARPGPDAPGRGTDYASSKESL
                     EASVLDCPDFLVFDLDPYIYSGKEAPGDEPELNTVAFEKGKEVAFHLRELLQSMSLEP
                     IVKTSGKTGLHVFVPIQRALDFDAAREVCELVGRHLLRLHPKDLTLEWSVPKRTGKIF
                     FDYNMNARGKTLNVAYSPRGAAGAPVSMPLTWEELATAHPLDFRINNAADRLAQTGDR
                     WRDALTRKQDLARALARAT"
     gene            3953..4672
                     /locus_tag="VFD06_07940"
     CDS             3953..4672
                     /locus_tag="VFD06_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZN03507.1"
                     /translation="MLTGTSGYSYKEWVGPFYPEKTPAPDMLRFYASRFPTVEINNTF
                     YRMPAAALLERWAQEVPEGFAFTLKAPRRITHIKRLKDTAEVVGEFFRRADALGDKLG
                     MTLFQCPPTLRKDLPRLREFLGALPVGRRAAFEFRHDSWNDDEVYEALRERSAAWCVA
                     EEDEGSTPFLCTSEDAYLRLRRTQYGETELRAWIDRLAAQPLERVWVYFKHEDEALAP
                     RFAAQFADLWSERSAARAGSR"
     gene            4669..>5505
                     /locus_tag="VFD06_07945"
     CDS             4669..>5505
                     /locus_tag="VFD06_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HZN03508.1"
                     /translation="MKRTGDTLRLSATDLANHLACRHLSHLDLAVARGLRRPPDWNSP
                     DAAILRERGEEHEDAFLTHLAAKGLRIARPGDGPDDDAGFARTLAAMREGADVIAQAT
                     LVDGSWFGRADVLCRVPLPSQLGDWSYEVWDTKLARETKGGSVLQICLYSDLLATIQG
                     QRPEHMYVVPPRDDFAPDRYRVDDYFAYYRLVRRRLEAAVRVPAADVEASGDGATLPL
                     FPEMPTYPEPVLHCDVCRWWPVCDKKRRDDDHLSLVAGISRLQRREMEAQRVPTLEAL
                     ASV"
BASE COUNT          786 a         1909 c         1897 g          913 t
ORIGIN      
        1 tgcgctggcg cggcacgcgg gcggtggcga gggcccgggc gctgcgcagg acggtcagat
       61 cctggaacga gaacagccac gtgttgcgtg gtccgcgcgc gggcgacacg agcccggcct
      121 cgacgagcga gcggatgcgg cggcgcggga ggccggcgag tttctcgacg tcgcgcacgc
      181 tgtactggtg catggcggga cggcccctcg ggtcctactt cttggcggtc ttcttcgggc
      241 gggtttccgc gtcctgggcg cgccggagcc ccttgcgacc gcccgcctgg gcctcccgcc
      301 cggcgtcctg ggtttcccgc gcagcggact ccggctcgcg cgccttgcct ggcgccttcc
      361 gctccaggct ggcccgcagc gcctccatga gatcgatgac ctggccgcct ccctccggga
      421 cctcggccat ggtgatctcc tggccctcga ccttcttctg caccgccgcc tcgatgcgcg
      481 tgcgcacttc gtccttgtac ggagcgggat cgaaggtgtc ggaggcctgc tgctcgatga
      541 gctgctgcgc cagcttcagc tcggcgtcct tgacgtcccc cggcgggatc tcgaggtcct
      601 cgagcgaccg cacctcgccg gcgtagagga gctgctgcat caccagcccg ccgttcaccg
      661 gccggatcat cacgatgtac tgcttcccgc gcgcggccca gcggccgatg gcgcacttgc
      721 cggactgctg cagggcccgg gcgagcaacg cgtacggctt gttcccgccc ttgtccggcg
      781 cgaggtagta ggccttgtcg aagtagaccg ggtcgatcga ggcgatcggc acgaactcgg
      841 agatgtccgc ggtgtgtgtc cccgcctcct ccatcgcctt gagctcctcc ggcgagaaca
      901 tgacgtactg gtccttggcg aactcgtacc ccttcaccat ctcgtcgcgc ggcaccggca
      961 cctcgtcctt ggcgcagagg tactgctgct tcagccgcga cccgcacccc ttgtgcagga
     1021 ggttgaagga gatggcgtgg ctcgcttcgg tcgccgagaa gagcttcacc gggatcgaga
     1081 cgagtccgaa cgagacggtc aaagacgcga tggcccgtgg tgccatggtt cctccggttt
     1141 cgtcaggtcg cgcgcgcgag ggcgcgggcg aggtcctgct tgcgtgtcag tgcgtcgcgc
     1201 caccgatccc cggtttgcgc caggcggtcg gcggcgttgt tgattcggaa gtcgagcggg
     1261 tgcgccgtgg ccaactcctc ccaggtcagc ggcatcgaca ccggcgcgcc ggcggcgccg
     1321 cgcggagaat atgccacatt cagggtcttg ccgcgtgcat tcatgttgta gtcgaagaag
     1381 atcttaccgg ttctcttcgg gacgctccac tccagggtga ggtccttcgg gtgcaggcgc
     1441 aggagatgcc ggccgaccag ctcacacacc tcacgcgccg cgtcgaagtc gagcgcgcgc
     1501 tggatcggca cgaagacgtg cagcccggtc ttccccgagg tcttgacgat cggctccagg
     1561 gacatgctct ggagcagctc gcgcaggtgg aatgccacct ctttcccctt ctcgaacgcc
     1621 acggtgttca gctccggctc gtcgccgggg gcttccttcc ccgaatatat gtacgggtcg
     1681 aggtcgaaga caaggaagtc gggacagtcg agcaccgaag cctccaggga ctccttcgac
     1741 gaagcgtagt cggtcccccg ccccggggcg tctggacccg gccgggcgcg cgagtgccag
     1801 acgtggaact cgagcgttcc caactgcgcc agccacagga gtgtcggcag gttgctgcac
     1861 atcaggtact cgtgcgattc ctccttgtgg ctggagaaga cggtgatcgt ctcgacgaac
     1921 tccgggcgct cctgctccca gtgcttctgg aagaagcggt gcccctggat cccgtccggc
     1981 atccggatca tggtgagcgg gcggtcggcg aggtgcggca gcatgtaggg cgagacctgc
     2041 gcgaggtagc gcagcagatc gcgcttggtc agaggcggct gctgcagtgc cgggtcggcg
     2101 ggccagtaga cgcggtcgag atgcgtcagc cggatgcggt gcgggccgac cacgaggggg
     2161 aagtccttcc gtgctgcatc cagctgcgcc aggatctcgc gcgttccgcc gccggcgtca
     2221 cccgcctggc cggcctcctc tttcccgggc gcggcggtcg ccggcgccga aaccgccacc
     2281 cggcgcacgt cccgaggccg caggtcgtcc cgtatcctta taaatacggg cgcgcgaaga
     2341 tgatcgtctt cggtccatga gtggaacttc acttcgacga cgatctccgg ctccacccag
     2401 gtggtcgggt tcggcagatc cggcttctcc gcgaacgggc aggcgtcgcg ccggagcggc
     2461 tccagacgtt tcttcaacgc cgtgaccgtc cgttcgtcga ggccggatcc cacgtgcgag
     2521 gcgaagatca gcttcttctt gtcccagtac ccgacgagga tcgctcccag gtccgagcgc
     2581 gaccccttcc ccttcgtgta gccgccgacg acaaaatcgg cgctcgcggt cggcttgatc
     2641 ttgagccagg tcgccgtgcg ccgccccgat tcgtagcggc tgtccttgcg cttgccgacg
     2701 accccttcga acccgctggc gagcgacgcc tggttcaggg cgacgccgtc ctccgtggcg
     2761 tgcaccggcc gcacgcgcgg cgtgggcagg aggcactggg cgaggtacct ccggcggtcg
     2821 cggtagggat gcttccgaag atcgactccg gcgaagaaga gcaggtcgaa gcagtagaag
     2881 accaccgaca cgtcccgctc cgccgccgcg atctccgccg gcgtcttcgc ctgaacgcgg
     2941 ttctggaggg cgttgaagga ggggcggccg tccagcccga gcgcgacgat ctcgccgtcg
     3001 aggatcatcg tcccggccgc ctgctgtccc agctcctcga tcaggcgcgg aaacgcttcc
     3061 ccgagatcat gcccccggcg cgagcgcagc cgcaccttcg cgccgtcgag gaatgccagg
     3121 gcccggtagc cgtcgagctt cggctcccac atccagtccg gatggttgaa cggcgcgggc
     3181 ccgatctcgg cgtgcatcgg ctcgagcgcc gccggcatcg gctcgacctt ccccgccggg
     3241 acgagccgcg aggccgggat gcggtgcgcc gggcgaacct tcatctcctc gacggtggct
     3301 ccggacagca ccgagcggtc ccgcgccgtg aggtcctcct tcgacacgaa gcggtccctg
     3361 tgcttgatca agagccacgt cttcgcgtct tttgtgcgca ccagcgccca cgagcctttc
     3421 accttctcgc cgcgcagcgt gaaccccagc ttccccttct cgatcccttc gcgcgcgcgc
     3481 ttctccgctt cggcgcgatc gtggaaccag gtgccaccct cgtccggaga ccagacgccg
     3541 cagtcccaga cgatcacctc tcccgcgccg tactgtcccg gcggtatgat cccctcgaac
     3601 gacgcatagt cataagggtg atcctcggtc cggacggcga gccgcttctc cccggggtcg
     3661 agggacgggc ctttcgggat gggccaggac ttcaggacgc cgtcgaactc caggcgcagg
     3721 tcgtaatgca tcgcgcgggc ggcgtgctgc tggatcacga agagcagcgg gccttcgcgc
     3781 tccgccatcg ccgggggcgg ctccggggtg gcgttgaagg cgcgcttggc ggcgtactcg
     3841 tcgagcgacg gggaggcggg aggcttcgga gcggcggact tcggaggcgg cgcggaagac
     3901 ttagggggtc gctggggagg cttggccttc ggcggtcgag gcaacgatgc ggatgttaac
     3961 aggaacgagc ggctattcct acaaggagtg ggtggggccg ttctacccgg agaagacgcc
     4021 ggcgcccgac atgctgcgct tctacgcctc gcgcttcccg acggtcgaga tcaacaacac
     4081 cttctaccgg atgccggcgg ccgcgctgct cgaacgttgg gcgcaggagg tgcccgaggg
     4141 gttcgcgttc acgctcaagg cgccgcgccg catcacgcac atcaagcgcc tgaaggacac
     4201 tgcggaagtc gtcggggagt tcttccggcg cgccgacgct ctgggggaca agctcggcat
     4261 gacgctcttc cagtgcccgc cgacgctccg gaaggatctc ccccggttgc gcgagttcct
     4321 cggggcgctg cccgtgggtc ggcgcgccgc cttcgagttt cgccacgatt cctggaacga
     4381 cgacgaggtg tacgaggccc tgcgcgaacg ctccgccgcc tggtgtgtgg ccgaggagga
     4441 cgagggctcg accccgttcc tctgcacgtc ggaggacgcc tacctccgcc tgcgccgtac
     4501 gcagtacggc gagaccgagc tgcgcgcctg gatcgatcgt ctcgcggcgc agccgctcga
     4561 gcgggtctgg gtctacttca agcacgagga cgaggcgctc gcgccgcgtt tcgccgcgca
     4621 gtttgccgat ctgtggagcg agcgcagcgc cgcgcgcgcg ggcagccgat gaagcggacc
     4681 ggcgacacgc tgcggctcag cgccaccgac ctggcgaatc atctcgcctg ccgccacctg
     4741 agccatctcg acctcgccgt cgcgcgcggc ctgcgtcgcc cgcccgactg gaactctccc
     4801 gacgccgcca tcctccgcga gcggggcgag gagcacgagg acgccttcct gacccacctc
     4861 gcggcgaagg ggctgcgcat cgcccgcccg ggagacggac ccgacgacga tgccggcttc
     4921 gcccgcacgc tcgccgccat gcgcgagggg gccgacgtga tcgcgcaggc cacgctcgtg
     4981 gacgggtcgt ggttcggccg ggccgacgtg ctgtgccgcg tgccgcttcc gagccagctg
     5041 ggtgactggt cgtacgaggt ctgggacacg aagctggcgc gcgagaccaa ggggggctcg
     5101 gtgctgcaga tctgtctcta ttcggatctg ctcgcgacca ttcagggaca gcggccggag
     5161 cacatgtacg tggtgccgcc acgggacgat ttcgcgcccg acaggtaccg ggtggacgac
     5221 tacttcgcct actaccggct ggtgcggcgc cggctcgagg cggcggtccg cgttcccgcc
     5281 gcggacgtcg aagcctccgg ggacggcgcc accctccccc tgtttcccga gatgccgacc
     5341 taccccgaac cagtgctcca ctgcgacgtc tgccgatggt ggccggtctg cgacaagaag
     5401 cgccgcgacg acgatcacct gagtctcgtg gcggggatca gccggctgca gcggcgagag
     5461 atggaggcac agagggtgcc gaccctcgaa gcgctcgcgt ccgtg
//
LOCUS       DASRIQ010000333         3508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3251865, whole genome shotgun sequence.
ACCESSION   DASRIQ010000333 DASRIQ010000000
VERSION     DASRIQ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3251865"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2099)
                     /locus_tag="VFD06_07950"
     CDS             complement(<1..2099)
                     /locus_tag="VFD06_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN03509.1"
                     /translation="MPRPVAWLALAAALGAAPLHASPRFPGRQHDVGVTPVAVAAADL
                     DGDGRIDLATANETSGDVALLLGRGAGDFAPAVRLASGLHPAAIAAADFDGDGRLDLA
                     AANSGSDEVTLFDGRGDGTFAPPRRFPAGAGPVALAVADLNGDRRPDLITANAYSDDL
                     SILAGVAGGGFAPEARLGLPSGPLSLAPADLDLDGSVDLVVALWVSEQIAFLRNRGDG
                     TFLPAVRTALPAAPIAVRAADFDRDGRPDLAVALHSGSIRLMPGRGDGSLGPAREVAA
                     VGTPRALETAFLDPGPDADLVVTDEQATLLFGRGNGTFEAPQRCGGLNGPAAVAAADF
                     DRDGGTDLVFANALANDPQTGWDDVGILRGRQDGTCVAPILSPAEDARSMSVADFDAD
                     GRRDAAMLLGEDGDLVLLRGEGDGTFSRSLPVAAGGGRGRLLAADFDGDARPDLARTH
                     TAADQVTIRLGRGDGTFGTPRTLGTVRAPAALATGRVDSDRIPDLVVAGSEQGEVSVF
                     LGKGDGTFGPESRLAAGVAPAAVAIGDLDGDRHGDLLVVNRGDDNLAVFGGRGDGSFD
                     APAFVPVGVAPTSLALHDLDGDGRSEAVIGFGRGSSVVVIRALGDGLFAALPIASVGL
                     APFAVAVGDLDGDGLADVVGGNLGSANVAVLPGAGVHGFASGAAFGAGRPRDLALADL
                     NGDGRKDVMALLGDAIAV"
     gene            2301..>3508
                     /locus_tag="VFD06_07955"
     CDS             2301..>3508
                     /locus_tag="VFD06_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03510.1"
                     /translation="MTFRRAWRAFKWSLLARHDRYPDFEIDRRLQRQERGTGVELAAL
                     RRDKTRALVRACLENVPYYGDLMRDARLDPDAIDGPEDLKVLPILTKEIVRREKGRML
                     NRAARPDEYHPHSTAGSTGLPLDFFRGYDYERIAVAGANLRAWRRMGWRPGDPMVHFW
                     QTHGDALPESRGLGRVRRGIRRWLEPPMEMLDPSDNSPAVLQQWVDRLRARGPDFCYG
                     YGSVLTLLALHLRNRGQTLERIRGLGTTAEALLPHARAALRDAFPRATLIDIYGSREV
                     PGVSAECRLGAMHVHSDLVHVEFIEDPQAQGGSRLALTALDNRIFPFIRYDIGDEGSP
                     PARACPCGLPFPVIRFGPGRIVDSFVMPDGRILYAGPLEDVMFEVPGLSRYQFLQKTA
                     GDIVLSVVPDA"
BASE COUNT          499 a         1319 c         1248 g          442 t
ORIGIN      
        1 acggcgatcg catccccgag gagggccatg acatccttgc gtccgtcgcc gttcagatcg
       61 gcgagcgcca ggtcgcgcgg gcgacccgca ccaaaggctg cgcccgaggc gaagccgtgc
      121 acaccggccc cggggagcac cgcgacattc gccgagccga ggttccctcc gacgacgtcg
      181 gcgaggccgt caccgtcgag atcgccgacg gcgaccgcga agggggccag tccgaccgac
      241 gcgatcggca gcgcggcgaa cagcccgtcc ccgagcgccc ggatcaccac gacactgctg
      301 ccgcggccga acccgatcac cgcctcgctg cggccgtcgc cgtcgaggtc gtggagcgcg
      361 agcgaggtcg gcgccacccc gaccggcacg aaggcgggcg catcgaagga tccgtcgccg
      421 cgccccccga acaccgcgag attgtcgtcg ccgcggttga cgaccaggag atcgccgtga
      481 cggtcgccgt cgagatcgcc gatcgccacc gcggccggcg ccacgcccgc ggcgagccgc
      541 gactccgggc cgaacgtgcc gtcccccttt ccgaggaaga ccgacacctc gccctgttcg
      601 gatccggcca ccacgagatc cggaatgcgg tcgctgtcga cccggcccgt cgcgagggcc
      661 gcgggggcgc gcacggtccc gagggtgcgc ggcgtcccga acgtgccatc gccgcgaccc
      721 aggcggatcg tcacctgatc ggcggccgtg tgggtccggg cgagatccgg gcgggcatca
      781 ccgtcgaaat cggcggcgag gaggcgcccg cgtccgcctc ccgcggccac cggcagggag
      841 cgggagaacg tgccgtcccc ctccccccgc agcagcacga gatcgccgtc ctcccccagg
      901 agcatcgccg cgtcacgccg tccgtccgcg tcgaaatcgg cgacgctcat cgagcgggca
      961 tcctcggccg gtgacaggat cggcgccacg caggtgccat cctgccggcc gcgcagaatg
     1021 ccgacgtcgt cccacccggt ctgggggtcg ttcgcgaggg cgttggcgaa gacgaggtcg
     1081 gtcccgccgt cccggtcgaa atcggcggca gcgaccgccg ccggtccgtt caggccgccg
     1141 cagcgctggg gcgcctcgaa ggttccgttg ccgcgaccga acagaagcgt cgcctgctcg
     1201 tcggtgacga cgaggtcggc gtccgggccg gggtcgagga acgcggtctc gagggcgcgg
     1261 ggcgtgccga ccgcggccac ctcccgcgcg gggcccaggc ttccgtctcc gcgtcccggc
     1321 atcaaccgga tcgatccgct gtgcagcgcc accgccaggt ccggccggcc gtcgcggtcg
     1381 aaatcggcgg cgcgtaccgc gatcggcgcc gcgggcaggg cggtccgcac cgccggcagg
     1441 aaggtgccgt cgccacggtt gcggagaaac gcgatctgct cggacaccca cagcgcgacc
     1501 acgaggtcca ccgacccgtc gaggtcgagg tcggccggcg ccagggacag aggcccggac
     1561 gggaggccga ggcgcgcctc cggcgcgaat cctccccccg ccacaccggc caggatcgac
     1621 agatcgtccg agtaggcgtt ggcggtgatg agatcgggac ggcggtcgcc gttgagatcg
     1681 gcgaccgcga gcgcgaccgg tccggcaccg gcagggaacc gtcgcggcgg cgcgaaggtt
     1741 ccgtcccccc gcccgtcgaa gagggtcact tcgtccgagc cgctgttcgc ggccgcgaga
     1801 tcgaggcggc cgtccccgtc gaagtcggcg gcggcgatcg ccgccgggtg caaccctgac
     1861 gcgagccgca ccgccggcgc gaagtcgccg gcgccgcggc cgaggagcag cgccacgtca
     1921 ccgctcgtct cgttggcggt cgcgagatcg atccggccgt cgccgtcgag atcggccgcg
     1981 gcgacggcga ccggcgtcac cccgacatcg tgctggcgcc ccggaaaccg gggcgacgcg
     2041 tgcagcggcg ccgccccgag cgccgcggcg agggccagcc aggccacggg ccgaggcacg
     2101 aaaccagtca cgggggtcac ggggctcatc gggatcgccg gcgcgagggc tccggcgcgg
     2161 cgggaatata ccccgaagcc gcgccacgtc tcgcgcgaag ttcgtaaccg ccgcgattac
     2221 gattcggtgc gggcgaacgg tgcgcggcgc gcggcgcgcg agagcggggt ccccccggcg
     2281 ggctgataga atgcgccgcc gtgaccttcc gacgcgcttg gcgcgccttc aaatggtcgc
     2341 tcctggcgcg ccacgaccgc taccccgatt tcgaaatcga caggcggctc cagcgccagg
     2401 agcgcgggac cggggtcgag ctcgctgcgt tgcggcggga caagacccgc gcgctggtcc
     2461 gcgcctgtct cgagaacgtt ccctactacg gcgacctcat gcgcgacgcc cgtctcgacc
     2521 cggacgcgat cgacggcccc gaggatctca aggtgctgcc gatcctgacg aaggagatcg
     2581 tgcgccgcga gaaagggcgg atgctcaacc gtgcggcccg cccggacgag taccaccctc
     2641 actcgaccgc cggttcgacc ggcctgcccc tcgatttctt ccgcggctac gactacgagc
     2701 ggatcgccgt cgcaggggcg aatctcagag cctggcggcg catgggctgg cgccctgggg
     2761 atccgatggt ccatttctgg cagacgcacg gcgacgcgct tccggagtca cgcggattgg
     2821 gccgcgtgcg gcgcgggatc cgccgctggc tcgagcctcc catggagatg ttggatccgt
     2881 ccgacaactc gccggcggtc ctgcagcagt gggtcgaccg gctgcgcgcg cgcggccccg
     2941 acttctgcta cggctacggg tcggtcctca cgctcctcgc cctgcacctg cgcaaccgcg
     3001 gtcagacact cgagcggatc cgcggcctcg gcaccaccgc ggaggcgctc ctccctcacg
     3061 cgcgcgccgc gcttcgggac gcatttcccc gggccacgct catcgacatc tacgggagcc
     3121 gggaggtccc gggggtctcc gccgagtgcc ggctcggcgc catgcacgtc cacagcgacc
     3181 ttgtccacgt cgagttcatc gaggacccgc aggcgcaggg agggtcacgc ctcgccctca
     3241 ccgcactcga caaccgcatc ttcccgttca tccgctacga catcggcgac gaagggagcc
     3301 cccccgcccg ggcgtgcccc tgcggcctgc cgttcccggt gatacggttc ggtccggggc
     3361 ggatcgtcga ctcattcgtg atgccggacg gtcgcatcct ctacgcgggg ccgctcgagg
     3421 acgtgatgtt cgaggtcccc ggcctgtccc ggtaccagtt cctgcagaag accgccgggg
     3481 acatcgtcct ctcggtggtt cccgatgc
//
LOCUS       DASRIQ010000334         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1446773, whole genome shotgun sequence.
ACCESSION   DASRIQ010000334 DASRIQ010000000
VERSION     DASRIQ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446773"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFD06_07960"
     CDS             <1..1120
                     /locus_tag="VFD06_07960"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HZN03511.1"
                     /translation="TVRYVDPDKNPAEVRRYNVTELGTLVVESGKQEQRVNSGDEEAI
                     TNALIKVTTDRERIVYSTTGHGEHDFNDTDRGGLSALKSALEKQNYVVKPLQLAAGVP
                     ADASAVIIDGPQKPFLEAEVKMISDWVEGGGRLLLMQDPGVDPGFGEMLARFGAAVRK
                     DVVIDKVSQLFGGDARIPMVPADGYDRSHPITKTFDYQTMYPLASSLDIKEPPPPDVT
                     AVKLARTSELSWGEVSEEEFQSGRIQLDDNDTRGPIALAAAFERKANTPAEHPAGTDG
                     APKPSAPGARLVLFGDGDFASNNYFNAGGNGDLALNAIAWLVEQGELMSIRPKSGTPR
                     IAILSAPQVFYYFWTIVAIAPLCIALVGVGIWWRRKRL"
     gene            1131..2603
                     /locus_tag="VFD06_07965"
     CDS             1131..2603
                     /locus_tag="VFD06_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HZN03512.1"
                     /translation="MKLRPGPVAALVLLAGLGGWAYVKDYRGADDRRKAEESKDKVFR
                     FERGDLTAIVLAGKNGPLRLEKRGEEWALAQPIAVAADKEAVEGIVSSLEMARVERRL
                     GPSSDLTTYALDPPSASVGIETKSGAPQTLALGDQAPLGGAWYALVDGKDGKEVAVVG
                     AAAGELAGRDLLALRDKSLLAFDPWKVVGVTLERGRDTVTLQKPEAGWKLQQPVEAPA
                     DGPAVTDLLSALERARATKFEVEKAGEADLKARGFLPPAARVTILQEGWDTAKTIEFG
                     AAATDGGRLARAVGKDAIVSLLPEFWSKLTAPAADFRRKDVLGVGQYRIRSVSASRDG
                     GPALVLTKGAESTWNASGLTQGPVKYDSVDLLLRRLSDVKAVGFDDRPADVVRQSLSV
                     KPPLDLVLEEEPDTEGGTPRRQHLSFGPIGKGARVVVRDFAWNPHFIVDGGAVAQVYQ
                     SLEDIPKEAAAAASAKPAAAPPAADGTPPVAEPPAAPPSN"
     gene            complement(2607..>3298)
                     /locus_tag="VFD06_07970"
     CDS             complement(2607..>3298)
                     /locus_tag="VFD06_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN03513.1"
                     /translation="RNDLDYALDPGAPEYSSGSGGTAMGARAFLFRPELIASAWGGSL
                     PFGRFPAPFSTGTSNADSDDDGVMDFLDTCPSILNPSQLDLDGDGFGNVCDCATGDPS
                     AWSLPGKAAFLFLGFDPLAGVGMTRLAWTAPTTGGLAASMTYDVIRSGKASDFMSAAS
                     CLGASLSGTMATDPEVPGPGSAFFYVVRPRDACGAGVSHHGSDGLPRPARDCQPVGGG
                     VPVPQPGPRKG"
BASE COUNT          528 a         1173 c         1164 g          433 t
ORIGIN      
        1 gaccgtgcgc tatgtggacc cggacaagaa cccggccgag gtgcggcgct acaacgtcac
       61 cgagctcggc accctcgtcg tcgagagcgg caagcaggag cagcgggtga attccggcga
      121 cgaggaggcg atcaccaacg ccctcatcaa ggtgacgacc gaccgcgagc ggatcgtgta
      181 cagcacgacc ggccatggcg agcacgactt caacgacacc gatcgcggcg gtctctccgc
      241 cctcaagtcg gccctggaga agcagaacta cgtggtcaag ccgctccagc tcgccgccgg
      301 cgtgccggca gacgcctccg cggtcatcat cgacgggccg cagaagccgt tcctcgaggc
      361 ggaggtgaag atgatctccg actgggtcga gggcggcggc cggctgctgc tgatgcagga
      421 cccgggcgtc gatcccgggt tcggagagat gctcgcccgg ttcggcgccg cggtccgcaa
      481 ggacgtggtg atcgacaagg tgtcgcagct cttcggcggc gacgcccgca tcccgatggt
      541 cccggcggac ggctacgacc gcagccatcc gatcaccaag accttcgact accagacgat
      601 gtacccgctg gcctcgtccc tcgacatcaa ggagccgccg ccgcccgatg tcaccgcggt
      661 gaagctggcc cgcacctccg agctgtcttg gggggaggtg agcgaggagg agttccagag
      721 cggccgcatc cagctcgacg acaacgacac gcgcgggccg atcgcgctcg ccgccgcctt
      781 cgagcgcaag gcgaacactc ccgccgagca tcccgccggg accgacggcg cgccgaagcc
      841 gtccgctccc ggcgcgcgcc tggtcctctt cggcgacggc gacttcgcca gcaacaacta
      901 cttcaatgcc ggcggcaacg gcgacctggc gctcaacgcc atcgcctggc tcgtggagca
      961 gggggagctg atgtcgatcc gccccaagtc cggaacaccg cgcatcgcca tcctgtccgc
     1021 gccccaggtc ttctactatt tctggaccat cgtggccatc gccccgctct gcatcgcgct
     1081 ggtcggcgtt ggcatctggt ggcgccgcaa gcgcctctga tcgcctcctc atgaagctcc
     1141 gccccggtcc ggtcgccgcc ctcgtcctgc tcgcggggct cggagggtgg gcctacgtca
     1201 aggactatcg cggcgccgac gatcggcgca aggccgagga gtccaaggac aaggtcttcc
     1261 ggttcgagcg cggcgacctc accgccatcg tcctcgccgg gaagaacggc cccctgcgcc
     1321 tcgagaagcg cggcgaggag tgggccctcg cgcagccgat cgcggtggcg gccgacaagg
     1381 aggcggtcga agggatcgtc tcgtcgctcg agatggcccg cgtcgagcgg cgcctcggcc
     1441 cgtcctccga cctcacgacc tacgcgctcg accctccctc cgcgagcgtg ggaatcgaga
     1501 cgaagagcgg agcgccacag acgctcgccc tcggcgacca ggcgcccctc ggcggggcgt
     1561 ggtacgccct cgtcgacggc aaggacggga aggaggtcgc ggtcgtgggc gcggcggccg
     1621 gggagctggc cggaagagat ctcctcgccc tgagggacaa atcgcttctg gccttcgacc
     1681 cgtggaaggt ggtcggcgtc acgctcgagc ggggccgcga caccgtcacc ctgcagaagc
     1741 cggaggccgg ctggaagctc cagcagccgg tcgaggctcc ggccgacggg ccggcggtga
     1801 ccgacctcct gtcggcgctc gaacgcgcgc gggccacgaa gttcgaggtc gagaaggcgg
     1861 gcgaggccga cctcaaggcg cgcggctttc ttcctccggc ggcccgcgtc acgatcctgc
     1921 aggaaggctg ggacaccgcg aagacgatcg agttcggcgc cgccgcgacc gacggcggac
     1981 gcttggcgcg cgcggtcggc aaggatgcca tcgtcagcct cctcccggaa ttctggtcga
     2041 agctgaccgc gcccgcagcc gacttccggc gcaaggatgt gctcggcgtc gggcagtacc
     2101 gcatccgatc ggtgagcgcc tcgcgcgacg gcgggccggc gctcgtcctc acgaaggggg
     2161 cggagagcac ctggaacgcc tccggtctca cccagggacc ggtgaagtac gactccgtgg
     2221 acctgctgct gcggcgtctc tcggacgtca aggcggtcgg cttcgacgac cgtccggccg
     2281 acgtcgtgcg tcagtcgctc tccgtgaagc cgccgctcga tctggtcctg gaagaggagc
     2341 cggacacgga gggggggacg ccgcgccgcc agcacctctc cttcggcccg atcgggaagg
     2401 gggcgcgcgt cgtcgtgcgc gattttgcct ggaaccccca cttcatcgtg gacggcggag
     2461 cggtcgcgca ggtctaccag agcctcgagg acattcccaa ggaggcggcc gccgcggcct
     2521 ccgccaagcc ggccgccgcg ccgccggccg cggacgggac accgccggtg gcggaaccgc
     2581 ccgccgcccc tccctcgaac taggtcctag ccctttctcg gccccggctg cggaaccggg
     2641 accccgcccc cgaccggctg gcagtcgcgg gccggccgcg ggagcccgtc ggagccgtgg
     2701 tgcgacacgc cggcgccgca ggcgtcgcgg ggccgcacca catagaagaa ggcggacccg
     2761 gggccgggca cctccgggtc ggttgccatc gtgccggaca gactcgcccc gaggcaggac
     2821 gcggccgaca tgaagtccga agctttgcct gatctgatga cgtcgtacgt catcgaagcg
     2881 gcgagaccgc cggtggtcgg ggcggtccag gccaggcgcg tcatccccac gccggccagc
     2941 gggtcgaacc cgaggaagag aaaagccgcc ttgccgggaa ggctccaggc cgaggggtcg
     3001 ccggtggcgc aatcgcagac gttcccgaag ccgtcgccat cgaggtcgag ctgcgaggga
     3061 ttgaggatcg acgggcaggt gtcgaggaag tccatcacac cgtcgtcgtc cgagtccgcg
     3121 ttcgacgtcc ccgtggagaa cggggcggga aatcgcccga agggcaaaga gccgccccag
     3181 gcggacgcga tcagctcagg acggaacagg aacgcacgcg cccccatcgc ggtccccccc
     3241 gagccggagg agtattccgg cgccccggga tccagggcgt agtcgaggtc gttccgat
//
LOCUS       DASRIQ010000335         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3526162, whole genome shotgun sequence.
ACCESSION   DASRIQ010000335 DASRIQ010000000
VERSION     DASRIQ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3526162"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="msrB"
                     /locus_tag="VFD06_07975"
     CDS             complement(<1..893)
                     /gene="msrB"
                     /locus_tag="VFD06_07975"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZN03514.1"
                     /translation="MTRSLSRPFLALLAAAALVACGAGASGEPAAAKDKEANMSDYHK
                     PTEEELRKRLTPLQFNVTQKDATEPSFRNEYWDNHEPGIYVDVVSGEALFSSMDKFES
                     GTGWPSFTRPLEPGNVAEHTDRKLFMTRTEVRSKHADSHLGHVFDDGPAPTGMRYCMN
                     SAALRFVPVDRLEAEGYGRYLPLFGKAAPAAASGGAAPVSTVTAGDRAGAAAAASDKG
                     KDSGRREEAILAGGCFWGMEEILREIPGVIDTEVGYTGGVTKTPVYEQVHAGTTGHAE
                     AVRVIFDPARLSYEDLLGWFFR"
     gene            complement(962..2008)
                     /locus_tag="VFD06_07980"
     CDS             complement(962..2008)
                     /locus_tag="VFD06_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN03515.1"
                     /translation="MMPTMKAIVLRQFGPPENLVLEEVPAPAAEALRPGEVLVRVEAV
                     GVCYHDLINRQGNLPRTKLPSIPGHEIAGTVFAVGPEVNGFLPGARVATIQRAPCGRC
                     DLCRRDRGSLCKEGVFFGEEIPGGYAQWVVAPVQALAPVPDGLATEEAAICACTLGTA
                     LHVARTRGRVQPGEQVLITGASGGVGLHAIQLVRHLGGRPIAVTSSEGKAARLREAGA
                     EEVIVSPDLKFAREVRRKTAGRGADVVLEITGALTFDQSLRSLAAGGRLVVVGNLETR
                     TVDLNPGLVILKELEIIGSFATSTSELREVFDLVRQGTLKPVVSQVLPLEEAASAHRS
                     LQERAVLGRVVLTV"
BASE COUNT          300 a          784 c          737 g          314 t
ORIGIN      
        1 cggaagaacc agccgaggag atcctcgtac gacagccgcg ccggatcgaa gatcacccgc
       61 accgcctcgg cgtgcccggt ggtcccggcg tgcacctgct cgtagaccgg ggtcttggtg
      121 acccctcccg tgtagccgac ctcggtgtcg atgactccgg gaatctcccg gaggatctcc
      181 tccatgcccc agaagcagcc gccggcgagg atcgcctcct cgcgccgccc cgagtccttc
      241 cccttgtccg acgccgcggc ggccgccccg gcccgatccc ccgccgtcac ggtcgatacg
      301 ggagccgccc cgcccgaagc cgcggccggc gccgccttcc cgaacagcgg caggtagcgg
      361 ccgtacccct ccgcctcgag ccggtccacc gggacgaagc gcagcgccgc ggaattcatg
      421 cagtagcgca tcccggtcgg cgccgggcca tcgtcgaaga cgtggccgag gtgcgagtcg
      481 gcgtgcttcg agcggacctc cgtccgggtc atgaagagct tgcggtcggt gtgctccgcg
      541 acgttgcccg gctccagggg gcgcgtgaac gacggccagc cggtccccga ctcgaacttg
      601 tccatggacg agaagagcgc ctcccccgag acgacgtcca cgtagattcc cggctcgtgg
      661 ttgtcccagt actcgttgcg gaaggacggc tcggtggcgt ccttctgggt cacgttgaac
      721 tgcaagggtg tcagccgctt ccggagttcc tcctccgtcg gcttgtggta atcgctcatg
      781 ttcgcctcct tgtccttcgc tgcggccggt tcccccgatg ctccggcgcc gcaggcgacg
      841 agggccgcgg cggcgagaag cgccaggaag ggtcgggaca ggctccgggt catcggccgc
      901 accatttcac cataccccct ttggatgcgt caccggggga aacgattcat ggtacgtccg
      961 gtcagacggt caggacgacc cggccgagaa cggcccgctc ctggagggac cggtgggccg
     1021 aggcggcctc ctcgaggggg aggacctgcg agaccaccgg cttgagggtc ccctgccgca
     1081 cgaggtcgaa cacctcgcgc agctcggagg tcgaggtggc gaaggagccg atgatctcca
     1141 gctccttgag gatgacgagc cccgggttca gatcgacggt gcgggtttcg aggttgccga
     1201 cgaccaccag cctccccccc gccgcgagcg accgcagcga ctggtcgaag gtgagggcgc
     1261 cggtgatctc gaggacgacg tcggcgccgc gtccggcggt cttccgccgc acttcgcggg
     1321 cgaacttgag gtccggagag acgatcacct cctccgcccc cgcctcccgc aggcgggccg
     1381 ccttcccttc cgacgaggtg acggcgatcg gccgcccgcc gaggtggcgc accagctgga
     1441 tggcgtgcag accgaccccg cccgaagcgc cggtgatgag gacctgctcc cccggctgca
     1501 cccgtccccg cgtgcgcgcc acgtgcaggg cggtgccgag cgtgcaggcg cagatcgccg
     1561 cctcctcggt cgccagcccg tccgggaccg gtgccagcgc ctgcacgggc gccaccaccc
     1621 actgcgcgta accgcccggg atctcctcgc cgaagaacac cccctccttg cacaggctgc
     1681 cgcgatcgcg ccggcacaga tcgcagcggc cgcacggggc ccgctggatg gtcgcgacgc
     1741 gcgctccggg gaggaagccg ttgacttcgg ggccgacggc gaagacggtg ccggcgatct
     1801 cgtgtcccgg gatgctcggg agcttggtgc gcggcaggtt cccctgccgg ttgatcaggt
     1861 cgtggtagca gacgccgacc gcctcgaccc gcaccaggac ctcccccggc cgcagcgctt
     1921 cggctgcggg ggcgggcacc tcctcgagca ccaggttctc ggggggaccg aactgtcgca
     1981 ggacgatcgc cttcatcgtc ggcatcatcg agacttcctc ccaggagctt cgtgcaatga
     2041 ggtgcgcggc gccggactca tccggccagc cggtgacgca tcccggccgg gtcgaggcgc
     2101 tcgatggcgt cccgctcggc gtcggtcggc tcggg
//
LOCUS       DASRIQ010000336         9066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3253964, whole genome shotgun sequence.
ACCESSION   DASRIQ010000336 DASRIQ010000000
VERSION     DASRIQ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3253964"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFD06_07985"
     CDS             complement(<1..326)
                     /locus_tag="VFD06_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03516.1"
                     /translation="MIDGRLQPITVPAAQEDLPLASPPLVPPVRLESRGEGIAHLVFE
                     PPPGKVNLLNASVISLLEVLVAQAAGQRLKGLIVTSTRPDQFIAGADVNEIRALRSFQ
                     DAEQASR"
     gene            complement(381..1670)
                     /locus_tag="VFD06_07990"
     CDS             complement(381..1670)
                     /locus_tag="VFD06_07990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN03517.1"
                     /translation="MTAPRRGVVILDGVRTPFCKAWGALEEVPADDLQRAPLAEAMAR
                     VDLDPKEVDEVVVGNIAQPADTTTLARVAALRAGVPKSVPAVTVNRNCGSGLEAIATA
                     WERIESGLADVVLAGGVESMSRIPFQMGQGFTRRVMGLRKARSAAARAGALLGFRRRD
                     ITPRSALEVGLRDAWSGLNMGETAEVLARRFQIGRAEQDAYALESHRRAVAARARLRE
                     EIVPLFPPPKHQPLLDDIGPREDQSLEALGRLKPVFDRRFGTVTAGNSCMLTDGAAAV
                     VVASEEKARALGTPWLGRIRAFAFAGLEPEVMGLGPVYAARRALERAGLRLSDMQLFE
                     INEAFAAQVLAVLKASASDAFGREALGLPGALGAIDPEILNVNGGAIALGHPVGCSGV
                     RLALTLLKEMARRDLTLGMAALCIGGGQGAALIVERS"
     gene            complement(1667..3118)
                     /locus_tag="VFD06_07995"
     CDS             complement(1667..3118)
                     /locus_tag="VFD06_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN03518.1"
                     /translation="MRARILVIDDEEAIRKSLRMVLEYEGYELIEAASGPEGLETMKR
                     ESPDAVLLDIKMPGMDGMEVLQQARARDAHTPILMVSGHGDIPTAVDALRKGAYDFLE
                     KPLESERVLAALRNATERKRLREENLRLKHQVEAGYRMIGEAPVLRSVREAIARAAPT
                     SATVLITGESGTGKELIAWDIHIRSARADKPFVKVNCAAIPEDLIESELFGHEKGSFT
                     GATGRQQGKFVQADGGTIFLDEIGDMSARTQAKVLRVLEGGEVEPVGLAKTVRVDVRV
                     IAATNKDLTSEIREGRFREDLFFRLNVVPIASPPLRDRREDIPELVAHFVDDFCADNS
                     YRRRTFSPEAMVRLQARPWRGNIRELRNFVERVLIMAEGETIEERDVVEVEQAAGGYP
                     AASPPPLPVPAGDGNAPAVLEPARPTPAGPDPAQFATLQQYREEAEKVFILRKLREFR
                     WNVSRMAKAIETPRSNLYKKLEQYGITRDQVQE"
     gene            complement(3241..5391)
                     /locus_tag="VFD06_08000"
     CDS             complement(3241..5391)
                     /locus_tag="VFD06_08000"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HZN03519.1"
                     /translation="MPRDTRTIVVIEDDPAALATYGRLLGRLGHQVLLRPASLEALAD
                     AALLKGSDLLILDQQMPGLSGLDLLERLRAGALELRRRPAALLVTAFADAALKARAAR
                     LGVEAVIDKPVDPARLLGTVERLLASREDEGGAAAMEGRGGPARAAGGGAFLTRPFRG
                     SYTELPSADTIPGGIKEPYSMQKHGRFVTLALIALGSVVFGMVLAGGLNLTLPGRAAD
                     AGVDSGRPLHAAALAQMNAGSSSPIPGSFADIAERVNPAVVSITATEVSDRSNRNRPF
                     FRGDPFEFFFGPQGPQQGPQPGPPSPHRRSPDQQDEPDIETSGGSGFLISDDGYILTN
                     YHVVEGATRIRVNLTDDRRDYNAEVVGSDPSTDLALIKIDVAKKLPYLSFGNSETLRI
                     GDWVIAIGNPLQYEHTVTVGVVSAKGRKLGGLSRDFSLDSFIQTDAAINFGNSGGPLI
                     NTSGQVIGVNTAISSVGQGIGFAVPAVIAQEVMNQLRIKGKVSRGFLGITVGEITPDM
                     AEAWGLKNDQGALVQSVSPGPAEQAGLRRGDIIVSIDGKPVTSSDEVVRLISARDPGS
                     KVRLTVLRDGKEIALTANLGERPNNAGGQRSGQGDEDQGPGEEENETRLGIRVEEMTP
                     SILNELGLPRDTKGVVITHVSRVSEAWEKQLNQGDVVVEVNRVPVPSLADYRREMRKA
                     KAGSLLVLYVINPPSRTGGDPVSRYVTLRVPKED"
     gene            complement(5551..6960)
                     /locus_tag="VFD06_08005"
     CDS             complement(5551..6960)
                     /locus_tag="VFD06_08005"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN03520.1"
                     /translation="MTAARGQIIVIDDDESNRKSTEMALRKDGYEVLACSGGEEGLEM
                     LKARGADLLVTDLRMPGLDGLQVLKRARLIDPALGVLVITGFGSVESAVDAMKQGADD
                     YLQKPVNLVELRKRVAAAVEKRRLAAEVASLKERLEEKFSFGRIIGSSPAMQQVLHQL
                     QLVAPTRSSVLIVGESGTGKELVANALHQNSPRKEARFVAVNAAAIPPDILESEMFGH
                     ERGAFTGALQRKIGTFEAADKGTLFLDEISELPLALQAKLLRVLEERSFMRVGGTETI
                     HVDVRILAATNADLEGRVAAGSFRSDLYYRLKVVTISLPPLRDRPEDVPVLTQRFFSL
                     FKEENARPDLTLDPEVLAVLQRARWDGNVRELRNLMESLVVLAPAGTTRVRVEDLPDS
                     YRPEHGGVRARPVAPAPPAAAPSPSRTMEEIEREAILRALAETGGNRTRAAELLGIGL
                     RTLQRKLKEYEQGSDDGAL"
     gene            complement(6957..8999)
                     /gene="ligA"
                     /locus_tag="VFD06_08010"
     CDS             complement(6957..8999)
                     /gene="ligA"
                     /locus_tag="VFD06_08010"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZN03521.1"
                     /translation="MNRAEAAERAETLRRDIWLHRKRYFVDNDPILSDGEYDLLEREL
                     RDLEERFPDLVTDDSPTQRVGAEVAGDLPTARHASPMLSLENVMDAEEFRAWRERLQR
                     VLDPDRTSPEPVLSAELKIDGTSLSLIYEDGRLARAVTRGDGTVGEEVTATVRTIPAV
                     PLRLLRPLPLLEARGEVFYPIAMFEEMNRRRAERGEAPFANPRNAAAGSLRLIDPALA
                     ASRPLDFFAWGLARIEGEPIPATHSAGLALLRDLGLRVAPHSGRVRGLEEAGAYFEER
                     RASRDGLPYEVDGCVFKVDDLRLQERAGFTARAPRWACAWKFPSRQATTTVLRIEVSV
                     GRTGALTPTAHLAPVVLGGATIQRATLHNEEEMRRRDVREGDTVLLERAGDVIPKIVK
                     SFPEQRGPDSKPFEWPTRCPVCGSEVVREEGEAISRCVNLSCPARLKESILHFARREA
                     MDIAGLGDALVDQLLARGLVKDLADLYHLDPGTLAGLERMGKKSADNLMRQIERSREV
                     PLERFLHGLGIRFVGERTAELLVDRFRSLDGIMEASLEELTGAREVGDRIAVAIRQFF
                     DREENADLLRRLRAAGLKLLPSPRAAVRDAGPFSGKAVVVTGTVPGWARDDIKRAVRE
                     GGGRITEGLSRKTDLLIAGEAPGSKLEKARGLGVRIMDAEEFRGIAGPGAGGPEAG"
BASE COUNT         1243 a         3285 c         3223 g         1315 t
ORIGIN      
        1 cgcgacgcct gctcggcgtc ctggaaggat cgcagggcgc ggatctcgtt gacatcggcc
       61 cccgcgatga actgatcggg gcgggtgctc gtgacgatga ggcctttgag gcgctgcccg
      121 gcggcctgcg ccaccaggac ctccaggagc gagatgacgg aggcgttgag gaggttcacc
      181 ttgccggggg gtggctcgaa gacgaggtgc gcgatcccct cgccgcgcga ctccaggcgg
      241 accggcggaa cgagcggcgg tgacgcgagg gggagatcct cctgcgccgc cggcaccgtg
      301 atcggctgca accgtccgtc gatcacgccg cgctctccgc gccggcttgg ctcatgcctc
      361 gctccccgcc tcggcctggc tcacgaccgc tccacgatga gcgccgcccc ctgcccgccg
      421 ccgatgcaca gcgccgccat cccgagcgtc agatcgcggc gcgccatctc cttgaggagc
      481 gtcagcgcca ggcgcacccc ggagcagccg accggatgtc cgagcgcgat ggcgccgccg
      541 ttgacgttga ggatctccgg atcgatggcg cccagggccc ccgggagccc cagcgcctcc
      601 cgtccgaagg catcggacgc ggaggccttg aggaccgcca gcacctgcgc ggcgaacgcc
      661 tcgttgatct cgaacagctg catgtcggag agccgcagac ccgcgcgctc gagggcgcgg
      721 cgggcggcgt acaccggccc gagccccatc acctccggct cgagcccggc gaacgcgaag
      781 gcgcggatgc ggccgagcca cggcgtgccc agggcgcgcg ccttctcctc cgaggcgacc
      841 acgacggcgg cggcgccgtc cgtcagcatg caggagttgc ccgccgtcac cgtcccgaaa
      901 cgccggtcga agaccggctt gaggcgcccg agcgcctcga gcgactggtc ctcgcgcggc
      961 ccgatgtcgt cgaggagcgg ctggtgcttc ggcggcggaa agagcggcac gatctcctcc
     1021 cgcaggcggg cccgcgccgc caccgcccgg cggtggctct cgagcgcgta ggcgtcctgc
     1081 tcggcgcggc cgatctggaa gcggcgcgcc agaacctccg cggtctcccc catgttgagg
     1141 ccgctccacg cgtcgcgcag cccgacctcc agcgccgagc gcggcgtgat gtcacgccgg
     1201 cggaacccga ggagcgcgcc ggcgcgggcc gccgccgagc gggccttgcg caaccccatg
     1261 acgcgacggg tgaacccctg ccccatctgg aacgggatgc gcgacatcga ctcgacgccg
     1321 ccggcgagga cgacgtccgc gagccccgac tcgatccgct cccaggcggt cgcgatcgcc
     1381 tcgagtcccg aaccgcagtt gcggttgacg gtgaccgcgg ggaccgactt cggcacgccg
     1441 gcccgcagcg ccgcgacccg cgccagcgtc gtggtgtcgg ccggctgcgc gatgttgccg
     1501 accaccacct cgtccacctc tttgggatcg aggtcgacgc gcgccatcgc ctccgcgagc
     1561 ggcgcgcgct gcaggtcgtc cgccgggacc tcctcgaggg ccccccatgc cttgcagaag
     1621 ggggtccgca cgccgtccag gatcaccacg ccgcggcgcg gtgcggtcat tcctggacct
     1681 ggtcccgcgt gatgccgtac tgctccagct tcttgtagag attgcttcga ggcgtctcga
     1741 tcgccttggc catgcgcgag acgttccagc ggaactcgcg cagcttccgg aggatgaaga
     1801 ccttctcggc ctcttcgcgg tactgctgca gggtggcgaa ctgcgccggg tcggggccgg
     1861 cgggcgtggg gcgggccggc tcgaggacgg ccggcgcgtt gccgtcccct gcggggaccg
     1921 gcagcggcgg cggggaggcc gcgggatagc cgcccgcggc ctgctccacc tcgaccacgt
     1981 cgcgctcctc gatcgtctcc ccctcggcca tgatgaggac gcgctcgacg aagttgcgca
     2041 gctcgcggat gttgccgcgc cacgggcgcg cctgcagccg caccatcgcc tccggcgaga
     2101 aggtgcgacg ccggtagctg ttgtcggcgc agaaatcatc gacgaagtgg gccaccagct
     2161 ccgggatgtc ctcacgccgg tcgcgcagcg gcggtgaggc gatcgggacg acgttcaggc
     2221 ggaagaacag gtcttcgcgg aaacgccctt cgcggatctc ggacgtcaga tccttgttcg
     2281 tggcggcgat gacgcgcacg tcgacgcgca ccgtcttggc cagcccgacc ggctcgacct
     2341 cccctccctc caggacgcgc agcaccttcg cctgggtgcg ggcgctcatg tcgccgatct
     2401 cgtcgaggaa gatcgtcccg ccgtcggcct ggacgaactt cccctgctgg cgaccggtgg
     2461 ctccggtgaa cgaccccttc tcgtggccga agagctccga ctcgatcagg tcctcgggga
     2521 tggcggcgca gttcaccttg acgaacggct tgtcggcgcg ggcgctgcgg atgtggatgt
     2581 cccaggcgat cagctccttg ccggtgccgc tctcgccggt gatgagcacc gtcgcggagg
     2641 tcggcgcggc gcgggcgatc gcctcgcgca ccgagcggag gaccggcgcc tcgccgatca
     2701 tccggtagcc ggcctcgacc tggtgcttga ggcgaaggtt ctcctcgcgc aggcgcttgc
     2761 gctcggtggc gttcctcagg gccgccagca cccgctcgga ctcgagcggc ttctcgagga
     2821 agtcgtaggc ccccttgcgc agcgcgtcca ccgccgtcgg gatgtcgccg tgtccggaaa
     2881 ccatcaggat cggcgtgtgc gcgtcgcggg cgcgcgcctg ctgcaggacc tccatgccgt
     2941 ccatgccggg catcttgatg tcgagcagca ccgcgtcggg cgactcgcgc ttcatggtct
     3001 cgagcccctc ggggccggag gccgcctcga tcagctcgta cccctcgtat tcgaggacca
     3061 tccggagcga cttccggatg gcctcttcgt cgtcgatgac caggatgcgg gcgcgcatga
     3121 ggtttgaagc cgcgccaagc ctcgcgcggc agtcgataat agcaaaaagc ggggcccgga
     3181 aatcccgggc cccgcctgca catcgagacg acgccccggc gcgtgccggg gacgcgtcga
     3241 tcagtcttcc ttcgggacgc gcagcgtcac gtagcgggag acgggatccc cgccggtgcg
     3301 gctgggagga ttgatgacgt aaagcaccag caggctaccc gccttggcct tgcgcatttc
     3361 gcggcggtag tcggccaggc tcggcaccgg aacgcggttc acctcgacca ccacgtcccc
     3421 ctggttcagc tgcttctccc acgcctccga gacccgcgac acgtgggtga tgacgacccc
     3481 cttggtatcg cgtggcagcc ccagctcgtt gagaatggac ggggtcatct cctcgacccg
     3541 gatcccgagc cgggtctcgt tctcctcctc gcccggcccc tgatcctcgt cgccctgccc
     3601 ggagcgctgc ccgccggcgt tgttcggccg ctcgccgagg ttggcggtga gggcgatctc
     3661 cttgccgtcg cgcaggacgg tcagacggac cttcgatccg ggatcgcgcg ccgagatcag
     3721 gcgcaccacc tcgtcgctgg aggtgaccgg cttgccgtcg atcgacacga tgatgtcgcc
     3781 gcgcctcaga ccggcctgct cggccgggcc cggcgacacc gactgcacca gcgccccctg
     3841 gtcgttcttg aggccccagg cctccgccat gtccggggtg atctcgccga ccgtgatccc
     3901 gaggaagccg cgcgacacct tgcctttgat gcgcagctgg ttcatcacct cctgcgcgat
     3961 caccgccgga accgcgaagc cgatcccctg cccgacgctg ctgatggcgg tgttcacgcc
     4021 gatcacctgt cccgaggtgt tgatcagcgg gccgccggaa ttgccgaagt tgatggccgc
     4081 gtcggtctgg atgaacgagt cgagggagaa gtcgcgcgac aggccgccga gcttgcgccc
     4141 cttggcgctc accaccccga cggtgacggt gtgctcgtac tgcagcgggt tgccgatcgc
     4201 gatgacccag tcgccgatgc gcagcgtctc ggagttcccg aacgacaggt aggggagctt
     4261 cttggccacg tcgatcttga tgagggcgag atcggtgctc gggtcgctcc cgaccacctc
     4321 ggcgttgtag tcgcgccggt catccgtgag gttgacgcgg atgcgcgtgg ccccctcgac
     4381 gacgtggtaa ttcgtcagga tgtatccgtc atcgctgatc aggaagccgg acccgccgct
     4441 cgtctcgatg tccggctcgt cctgctggtc gggggaccgc cggtgcggcg acggcggccc
     4501 gggctgcggt ccctgctgcg gcccctgcgg gccgaagaag aactcgaacg ggtccccgcg
     4561 gaagaacggc cggttgcggt tggagcgatc tgagacctcg gtggcggtga tgctgacgac
     4621 cgccgggttg acccgctccg cgatgtcggc gaacgagccg gggatcgggg aggacgatcc
     4681 ggcgttcatt tgggccagcg ccgcggcgtg cagcgggcgg ccgctgtcga ctccggcgtc
     4741 ggccgcgcgc cccgggaggg tcaggttcag gcctcccgcg aggaccatcc cgaagacaac
     4801 cgaccccagc gcgatgagcg ccagcgtcac gaagcgaccg tgtttctgca tggagtacgg
     4861 ctccttgatc ccgcccggaa tcgtgtcagc cgaagggagt tcagtatagg acccgcggaa
     4921 aggccgggtc aagaaagccc caccgcccgc cgcgcgggcc gggcccccgc gcccctccat
     4981 cgcggccgct cccccctcgt cctcgcgcga cgcgaggagg cgctcgaccg ttcccagcag
     5041 gcgggccggg tcgaccggct tgtcgatcac cgcctcgaca ccgagccgcg cggcgcgcgc
     5101 cttgagcgcc gcgtcggcga acgccgtgac caggagggcg gccggccgac ggcggagctc
     5161 caaggccccc gcgcgcaggc gctcgagcag gtcgaggccc gacagccccg gcatctgctg
     5221 gtcgaggatc aggaggtcgg accccttcag gagcgccgcg tcggccaggg cctcgaggga
     5281 ggccggtcgg agcaggacct ggtgaccgag ccggccgaga aggcggccgt aggtggcgag
     5341 cgccgccggg tcgtcctcga tgacgacgat ggtcctggtg tcgcgaggca tgatcggagg
     5401 cggaaacagc aagcgttgtg ccacgcagtg agcaggtaaa accgctctgc gcagcccggt
     5461 ccgggggttt ggacgggaca gaacgccgcc cgcggggctc gcctgcgcca aattgacgcc
     5521 ggcggcggcg gacgcgcccg gcgcgacctc ttagagggcg ccgtcgtcgc tgccctgttc
     5581 gtactccttg agcttccgct gcagggtgcg cagcccgatc ccgaggagct cggcggcccg
     5641 ggtgcggttt ccgccggtct cggcgagcgc ccggaggatc gcctcccgct cgatctcctc
     5701 catcgtccgg gagggggacg gggcggcggc gggcggcgcg ggagcgaccg ggcgggcgcg
     5761 cacgccaccg tgttccgggc ggtaggagtc gggaaggtct tcgacccgga cgcgcgtggt
     5821 cccggccggc gcgagcacca cgaggctctc catgagattg cgcagctcgc gcacgttgcc
     5881 gtcccagcgg gcccgctgca ggaccgcgag gacttccgga tcgagggtca ggtcgggacg
     5941 cgcgttctcc tccttgaaga gggagaagaa gcgctgcgtc aggaccggga cgtcctcggg
     6001 acgatcccgc agcggcggca gggagatggt gacgaccttg agccggtagt acaggtcgct
     6061 ccggaagctc ccggcggcga cgcggccctc gagatcggcg ttggtggcgg cgaggatgcg
     6121 cacatcgacg tggatcgtct cggtcccccc cacacgcatg aaggagcgct cctcgaggac
     6181 ccgcagcagc ttcgcctgca gcgccagcgg cagctcgctg atctcgtcga ggaacagcgt
     6241 ccccttgtcc gccgcctcga aggtgccgat cttccgctgc agggcgccgg tgaacgcgcc
     6301 gcgctcgtgg ccgaacatct cgctctccag gatgtcgggc gggatggcgg cggcgttcac
     6361 cgccacgaag cgggcctcct tgcgcggcga gttctggtgc agggcgttgg cgaccagctc
     6421 cttgccggtg ccgctctcgc cgacgatgag gacgctcgac cgtgtcggcg ccacgagctg
     6481 cagctggtgc aggacctgct gcatcgccgg ggaggacccg atgatgcgcc cgaaggagaa
     6541 cttctcctcg aggcgctcct tgaggctcgc cacctcggcc gcgaggcggc gcttctccac
     6601 cgccgcggcg acgcgcttgc gcagctcgac gaggttgacc ggcttctgga ggtagtcatc
     6661 cgccccctgc ttcatggcgt cgaccgccga ctcgaccgag ccgaacccgg tgatcaccag
     6721 cacgccgagg gcggggtcga tgagtcgggc gcgcttgagg acctggagac cgtcgaggcc
     6781 gggcatgcgg aggtcggtga cgagcaggtc ggcgccgcgc gccttgagca tctccagccc
     6841 ctcctcccct ccggagcagg cgaggacctc gtatccgtcc ttgcggagcg ccatctcggt
     6901 ggacttgcga ttgctctcgt cgtcgtcgat gacgatgatc tggccgcgcg ccgccgtcat
     6961 cctgcctccg gtccgccggc gccggggccg gcgatgccgc ggaactcctc cgcgtccatg
     7021 atccgcaccc ccaggccacg cgccttctcc agcttcgatc ccggcgcctc gccggcgatc
     7081 agcaggtcgg tcttgcgcga caatccctcg gtgattcgcc cgccgccctc gcgcaccgcc
     7141 cgcttgatgt cgtcgcgcgc ccaccccggc accgtgccgg tgacgacgac cgccttgccg
     7201 gagaacggcc cggcatcccg gaccgcggcc cgcggcgaag gcaggagctt cagcccggcc
     7261 gcacgcaggc gccggaggag atcggcgttc tcctcgcgat cgaagaactg ccggatggcg
     7321 acggcgatcc ggtccccgac ctcgcgcgcc ccggtgagct cctcgaggga ggcctccatg
     7381 atgccgtcga gcgaccggaa gcggtcgacc agaagctcgg cggtgcgctc ccccacgaag
     7441 cggatgccga ggccgtggag gaagcgctcc aggggcacct cgcggctgcg ctcgatctgc
     7501 cgcatcagat tgtcggccga cttcttcccc atgcgctcca ggccggcgag ggtcccgggg
     7561 tcgagatgat agaggtccgc gaggtccttc acgagcccgc gcgccagcag ttgatcgacg
     7621 agcgcgtcgc cgagtccggc gatgtccatc gcctcgcgcc gggcgaagtg gaggatcgac
     7681 tccttgagcc gcgccgggca ggagaggttg acgcagcggc tgatcgcctc cccctcctcg
     7741 cgcacgacct ccgacccgca gacggggcac cgcgtcggcc actcgaacgg cttcgaatcc
     7801 ggaccgcgct gctcggggaa cgatttgacg attttcggga tgacgtcgcc ggcccgctcc
     7861 aggaggaccg tgtccccctc ccgcacgtca cgccgccgca tctcctcctc gttgtgcagc
     7921 gtcgcccgct ggatggtcgc cccgccgagg acgaccggcg cgaggtgcgc cgtcggggtc
     7981 agcgccccgg tccgccccac cgacacctcg atgcgcagga cggtggtcgt cgcctggcgc
     8041 gacgggaact tccaggcgca ggcccagcgc ggcgcgcgcg cggtgaaccc ggcccgctcc
     8101 tggaggcgga ggtcgtccac cttgaagacg cagccgtcca cctcgtacgg caggccgtcg
     8161 cgcgacgcgc ggcgctcctc gaagtacgcc cccgcctcct cgaggccgcg gacccggccc
     8221 gagtgcgggg cgacccggag gccgagatcc cggaggagcg ccagcccggc gctgtgcgtc
     8281 gcgggtatcg gctccccctc gatacgggcc agcccccagg cgaagaagtc gagcgggcgc
     8341 gaggcggcca gcgccgggtc gatgaggcgc agcgagccgg ccgccgcgtt gcgcgggttg
     8401 gcgaacggtg cctccccccg ctccgctcgc cggcggttca tctcctcgaa catcgcgatc
     8461 gggtagaaca cctcgccgcg cgcctccagg agcggaaggg ggcgcagcag gcgcagcggc
     8521 accgcgggga tggtgcggac cgtcgcggtc acctcctcgc cgacggtccc gtcgccgcgg
     8581 gtgacggcgc gcgccaggcg cccgtcctcg tagatgaggg acagggaggt gccgtcgatc
     8641 ttgagctcgg ccgacaggac cggctccggg gaggtccgat cggggtcgag gacgcgctgc
     8701 agtcgctcgc gccacgcgcg gaactcctcc gcgtccatga cgttctcgag gctgagcatc
     8761 ggcgaggcgt ggcgcgccgt cggcaggtcg ccggcgacct ccgcgccgac ccgctgcgtc
     8821 ggcgaatcgt cggtgacgag gtccgggaag cgctcctcca gatcgcgcag ctcccgctcg
     8881 aggaggtcgt actccccgtc cgacaggatc gggtcgttgt ccacgaagta ccgcttgcgg
     8941 tgcagccaga tgtcgcggcg cagcgtctcc gcccgctccg cggcctcggc gcgattcacg
     9001 tccgcgacac cgccaccggg ccggggagag cccccgattc gggccgctgc cggcgcggcc
     9061 agcgga
//
LOCUS       DASRIQ010000337         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_384807, whole genome shotgun sequence.
ACCESSION   DASRIQ010000337 DASRIQ010000000
VERSION     DASRIQ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384807"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFD06_08015"
     CDS             <1..324
                     /locus_tag="VFD06_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZN03522.1"
                     /translation="DLPAEVRGTTHAWHLYILRLRPGVLTIGRDRFSDLLGERGIGHS
                     VHFLPLHLHSYYRRTFGYRPGAFPHTERESGRALSLPLYPGLALRDQDRVIAEVLRLA
                     ALHLR"
     gene            321..962
                     /locus_tag="VFD06_08020"
     CDS             321..962
                     /locus_tag="VFD06_08020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZN03523.1"
                     /translation="MTGSGRGRAYCRGPLKRALDLSVSALALAVFAPLLLLAALLVLL
                     TSGRPVLFRQERIGLEGRRFRILKFRTMRDGPGGTAITGAGDRRITPAGALLRASKID
                     ELPQFFNVLKGDMAIVGPRPEIPRFVAGYSAAERRVLDVRPGLTDLATLAYRDEERLL
                     GTVAEGDRERFYASEVLPKKLLLNLQYVERASLREDLAIVLRTAAAIVLPERR"
     gene            959..2101
                     /locus_tag="VFD06_08025"
     CDS             959..2101
                     /locus_tag="VFD06_08025"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN03524.1"
                     /translation="MIVLFALGLGALLHAYLIYPLSMQAVALLRPRRRPATAPADARE
                     WPSITLVISAYNEERVLREKLENVAALDYPAGRLQAIVVSDGSTDETEAIARAFAPQG
                     VALRAFTGRQGKVACLNRVLPDVRSDLVVMSDANSLYEPGSLRRLAEHFADARIGCVC
                     GELRYRNTRELSSGEGERAYWEYERWVKRVEGASGSLLGANGAIYAFRSPLFRQVDPL
                     MFCDDVIPVRIRIGGADVIYEPGALCLEEAVDERTERRRRRRHASFGLRSMLALCREA
                     ASSGRPLVVYQCVSHRILRWFGGLWLFLILVGTSGLPAPYFQAAASAQGALYGAAAVG
                     WVLDRLGRRVTILYLPFYALAITAAGILGLVHYFRGTDRPWWEPRQ"
     gene            2230..>2722
                     /locus_tag="VFD06_08030"
     CDS             2230..>2722
                     /locus_tag="VFD06_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03525.1"
                     /translation="MTRLRRLLLPLGDLLAVVAANLVAAALRFEFDPSQIQQGDHHIL
                     EVMALDLLVTPIVFRLCGLYRAYWKYAGLDDLVRLARAVAVRALTLVVVIYGWDVQGA
                     SRAILILASVLLVAFAGGIRLLPRFTQEMTASRRRVGGRRTLIVGAGDTGESLLRELR
                     KGGE"
BASE COUNT          338 a          956 c          955 g          473 t
ORIGIN      
        1 gatctgccgg ccgaggtgcg cggcacgacc cacgcctggc acctgtacat cctgcgcctc
       61 cggccgggcg tcctgacgat cgggcgcgat cggttctccg acctgctcgg cgagcgcggc
      121 atcgggcaca gcgtccactt cctgcccctt catctgcatt cgtactatcg ccggaccttc
      181 ggttaccgcc cgggtgcgtt tccacacacg gaacgcgagt ccgggagggc gttgtctctc
      241 ccgctgtacc ccgggctggc gctgcgcgac caggatcggg tgatcgcaga ggtcctccgg
      301 ctggcggcgc tgcatctcag atgacggggt cggggcgcgg ccgggcctac tgccgcgggc
      361 ccctgaaacg cgccctggac ctgtccgtgt ccgcgctggc gctcgcggtc ttcgcgccgc
      421 tcctgcttct ggccgcgctc ctggttctcc tcacctccgg tcgcccggtg ctcttccggc
      481 aggaacggat cgggctcgaa ggacggcgat tccggatcct caagttccgg acgatgcgcg
      541 acggtccggg ggggaccgcg atcaccggcg cgggcgaccg ccggatcacg ccggccggcg
      601 cgctgctgcg cgcgagcaag atcgatgagc tgccgcagtt cttcaacgtc ctgaagggag
      661 acatggcgat cgtcgggccg cggccggaga tcccgcgctt cgtcgcgggc tacagcgcgg
      721 ccgagcgccg ggtcctcgac gtgcgccccg ggctcaccga cctcgcgacg ctcgcctacc
      781 gcgacgaaga gcggctcctc ggaacggtgg cggaggggga ccgcgagcgt ttctacgctt
      841 cggaggtctt gccgaagaaa ctcctcctga acctccagta cgtcgagcgc gcctcgctgc
      901 gcgaagacct cgccatcgtg ctccggacgg cggccgccat cgtcctgccg gagcggcgat
      961 gatcgtcctg ttcgcgctcg gtctgggagc gctcctgcat gcgtatctga tctatccgct
     1021 cagcatgcag gcggtcgcgc tcctgaggcc gcgccggcgc cccgcgaccg cacccgccga
     1081 cgcccgggag tggccgtcga tcacgctggt gatctccgcc tacaacgagg agagggtgct
     1141 gcgcgagaaa ctcgagaacg tcgccgccct cgactatcct gccggacgcc tgcaggccat
     1201 cgtcgtctcc gacggctcga ccgacgagac cgaggccatc gcccgcgcgt tcgcgccgca
     1261 gggcgtcgcc ctgcgcgcgt tcaccggccg ccaggggaag gtcgcctgtc tcaaccgcgt
     1321 gctgccggac gtccggagcg acctcgtggt catgtcggac gccaactccc tgtacgagcc
     1381 gggaagcctc cgccgcctcg cggagcactt cgccgacgct cggatcggct gcgtctgcgg
     1441 cgagttgcgc taccgcaaca cgcgggagct ttcgtccggg gagggggagc gggcgtactg
     1501 ggagtacgag cgttgggtca agcgggtcga gggggcgagc ggatcgcttc tgggtgcgaa
     1561 cggggccatc tatgcgttcc gatcgccgct gttccgccag gtcgatccgc tgatgttctg
     1621 cgacgacgtc atcccggtcc gcatccggat cggaggagcc gacgtgatct acgagcccgg
     1681 ggcgctctgc ctggaggagg cggtggacga gaggaccgaa cgacgccggc gccggcggca
     1741 tgcctcgttc ggcctgcgct ccatgctcgc cctgtgccgc gaggccgcct cctcggggcg
     1801 gcccctcgtc gtttaccagt gtgtctccca ccggatcctg cgctggttcg gcgggctgtg
     1861 gctgttcctg atcctcgtcg ggacctccgg gctcccggcg ccgtacttcc aggcggcggc
     1921 gagcgcccag ggcgccctct acggggcggc ggcggtggga tgggtgctcg atcgcctggg
     1981 ccggcgtgtg acgattctgt atcttccgtt ctacgcgctg gcgatcaccg cggccgggat
     2041 cctggggctg gtccattact tccgcggcac cgatcgtcca tggtgggaac cccggcaata
     2101 gcctgattct gcaggcattt ggttgacaat ccggtcgtcc cgtcgtatac agacgccgac
     2161 ccggaaagac ccaccaggcg ggggcaggtg tgctccgccg gccggatggt ccggccgggt
     2221 gtccgctgcg tgactcgact tcgccgcctg cttcttccgc tcggcgatct tctcgccgtg
     2281 gtggccgcca atctggtggc ggccgcgctg cgcttcgaat tcgatccgtc gcagatccag
     2341 caaggggatc accacatctt ggaggtgatg gccctcgatc tgctggtgac gccgatcgtc
     2401 ttccggctgt gcgggctgta tcgcgcctac tggaagtacg ccgggctcga cgacctcgtc
     2461 cggctcgccc gggcggtcgc cgtccgcgcg ctgacgctcg tcgtggtgat ctacggctgg
     2521 gacgttcagg gcgcctcgcg cgccatcctc atcctcgctt cggtcctcct ggtcgcgttc
     2581 gccgggggca tccggctgct gccgaggttc acccaggaga tgaccgcttc gcggcggcgg
     2641 gtcggcggac gcaggacgct catcgtcgga gccggggaca ccggggagtc gctgctccgg
     2701 gaacttcgca aggggggcga gg
//
LOCUS       DASRIQ010000338         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1955805, whole genome shotgun sequence.
ACCESSION   DASRIQ010000338 DASRIQ010000000
VERSION     DASRIQ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955805"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VFD06_08035"
     CDS             complement(<1..840)
                     /locus_tag="VFD06_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN03526.1"
                     /translation="MRSGSKLAGARRLVARLAGALGLVLLASQASLGDPPGDGILFGP
                     VVTSVGARPTDSAIADFDGDGTLDAAIADGPFNQISILLGNGDGTFVVTGQSPFGSEM
                     AGVAAADIDGNGTIDIIGERQSVDLVGVLKGNGDGTFQAATYLAAGDGPRAIATGDFT
                     SDGIADVLVANEFANDLSFYRGSAGGLLPEVRLSVGTQPVAIAVGDVNGDGARDAAVL
                     LKTAKQVVVLLGNGTGNFPASSVLPLGGDPSDLALGTIDADGVVDLVVDYYCGDQFCQ
                     TPGT"
     gene            1049..1234
                     /locus_tag="VFD06_08040"
     CDS             1049..1234
                     /locus_tag="VFD06_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03527.1"
                     /translation="MPRTATSYRFRLGLAALALAALASPAWASLPQVANLQVQTIVSP
                     RLFTWPAWAWKNPGPQQ"
     gene            complement(1273..2553)
                     /gene="serS"
                     /locus_tag="VFD06_08045"
     CDS             complement(1273..2553)
                     /gene="serS"
                     /locus_tag="VFD06_08045"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZN03528.1"
                     /translation="MLDRTWVRDHLDEVETAMKKRGAAVDIEAIRRLDSERRKALHEV
                     EILKARRNAVSGDIARLKKEGKDAAALIDEMRGAGDRIKELDARSDEADEGLEALLLN
                     VPNLPHPSVPAGRTSDDNVEVRRWGTLPKFSFEAKAHWDLGAALGILDFERAARMTGA
                     RFVAYMGAGARLERSLIQFMLDLQTKEHGYTEVLPPFMVNEKAMTGTGNLPKFAGDLF
                     KIEGTGYYLIPTAEVPLTNLHREEILDGDRLPLSYTAWTPCFRSEAGSYGKDVKGLIR
                     QHQFNKVELVKFTKPEDSMAALEQLTKDAETVLQRLGLPYRVIALCTGDMGFASMKTY
                     DIEVWLPSFDAYREISSCSNCGDFQARRAGIRFRRGPSGKTELAHTLNGSGLAVGRTL
                     VAILENGQRADGSIAIPEALVPYFEAAEITPASR"
     gene            2582..3223
                     /locus_tag="VFD06_08050"
     CDS             2582..3223
                     /locus_tag="VFD06_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03529.1"
                     /translation="MARTAKADYQRSLKVTTLPLPDPDAAARTALHVLDAPDAAGRLA
                     AGQRLLDLLAAQTSISTPELVVPDSPQPHRRSGGRIVYSLQGDYRRRQPSKDDPAVAR
                     GGKPLGRIRVPHRTPARGDVVRPTAFLHTLLHEFCHHHDAESLGLFRSFHTAGFYARL
                     RDLRSQVEPVVLEPPRRTPERRRKSRGPAAPTKAAPPLQLSLWSALRAIFKRS"
     gene            3306..>4264
                     /gene="galU"
                     /locus_tag="VFD06_08055"
     CDS             3306..>4264
                     /gene="galU"
                     /locus_tag="VFD06_08055"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HZN03530.1"
                     /translation="MSTQASQPRPPRVRKAVFPAAGLGTRFLPATKALPKEMLPLVDK
                     PVIQYVIEEAVDSGIESIIIVTGRGKSAIEDHFDISFELESLLERRGKHDLLREVRTI
                     SDLINVSYVRQKEPLGLGHAILVARDLVGPEPFAVFLGDDIIHAPVPVMQQMIDVHAR
                     TGSSVIGVERVARERSRDYGMVAVDEPGAGRTYRITDLVEKPPPEEAPSDLAIIGRYI
                     LTPEIFPHLERTGAGRGGEIQLTDGLRSLLKEQPITAYAFEGIRYDTGDKLGFLKATV
                     EFALRRPDLGGPFRAYLEQLSLDGDAVRPVAKPDVAPRAARPAR"
BASE COUNT          626 a         1561 c         1410 g          667 t
ORIGIN      
        1 cgtccccggc gtctggcaga actggtcgcc gcagtagtag tccacgacga gatcgacgac
       61 accatccgcg tcgatggtcc cgagggccag gtcggaggga tccccgccga gggggagaac
      121 ggacgacgcg gggaagttcc ccgtgccgtt ccccagcagc acgacgacct gtttcgcggt
      181 cttcaggagg acggcggcat cgcgcgcacc gtcgccgttc acgtccccga ccgcgattgc
      241 caccggctgg gtcccgacgc tgaggcggac ttcgggcagc aatccgccgg cagagccccg
      301 gtagaaggaa agatcgttcg cgaattcgtt cgcgaccagg acatcggcga tcccgtccga
      361 ggtgaaatca ccggtcgcga tcgcccgcgg gccgtccccg gccgcgaggt aggtggccgc
      421 ctgaaacgtg ccgtcgccgt tccccttcag gaccccgacg aggtcgacgc tctgacgttc
      481 cccgatgatg tcgatggtcc cgttcccgtc gatgtcggcc gccgcgaccc ccgccatctc
      541 cgagccgaac ggggactgcc cggtgacgac gaacgtcccg tccccgtttc cgagaaggat
      601 ggagatctgg ttgaacggcc cgtcggcgat ggcggcgtcg agcgttccgt ccccatcgaa
      661 gtcggcgatc gccgagtcgg tcggacgcgc gcctacggag gtgacgacgg gcccgaagag
      721 gatcccatcg ccgggcggat caccgagact ggcctgggag gccagcagga cgagcccgag
      781 cgctcctgcc agcctcgcaa cgagacggcg cgctccagcg agtttcgatc cggatcgcat
      841 acatcctccc gacggaccgg ctgacacacc gcgagcgagc gacgattggt cctctgtata
      901 ccgcgcctcc ccacccgccg caaccggaat cttgcgacac ctatgtcgca ctttggccgc
      961 cgtttgcgtt taatccaagg aagacgttgc cgtgcccgaa aggaggggta gattctgagg
     1021 actcgcacct cgccgggggg ggaggatgat gccacggacc gccacgtcgt accggttcag
     1081 gcttgggctg gcggcgctcg ccctggcggc cctcgcctcg ccggcctggg cctccctgcc
     1141 ccaggtcgcc aaccttcagg ttcagaccat cgtctcgccg aggttgttca cgtggccggc
     1201 atgggcttgg aagaaccccg gcccgcaaca gtaggatgcg ttggagcagc cgtcgcggat
     1261 cgaggatccg cgctaccgcg atgcgggggt gatctccgcc gcctcgaagt acggcacgag
     1321 cgcctccggg atggcgatcg acccgtcggc ccgctggccg ttctcgagga tggcgacgag
     1381 cgtccgcccg accgccagcc ccgagccgtt cagcgtgtgg gccagctcgg tcttcccgga
     1441 cgggccgcgg cggaagcgga tgccggcgcg gcgcgcctgg aagtcgccgc agttcgagca
     1501 cgacgagatc tcccggtagg cgtcgaacga cggcagccac acctcgatgt cgtaggtctt
     1561 catcgaggcg aatcccatgt cgccggtgca caaagcgatg acgcggtacg gcagcccgag
     1621 gcgctgcagc accgtctcgg cgtccttcgt caactgctcg agcgccgcca tcgagtcttc
     1681 cggcttggtg aatttcacca gctccacctt gttgaactgg tgctgccgga tgagcccctt
     1741 gacgtccttg ccgtacgagc cggcctcgct gcggaagcag ggcgtccagg cggtgtacga
     1801 cagcggcagc cggtcgccgt cgaggatctc ctcgcggtgc aggttggtga gcggcacctc
     1861 ggcggtcggg atcaggtagt agccggtccc ctcaattttg aagaggtcgc cggcgaattt
     1921 cggcaggttg ccggtgccgg tcatcgcctt ctcgttgacc atgaacggcg gcagcacctc
     1981 ggtgtagccg tgctccttcg tctgcagatc gagcatgaac tggatgagcg accgctccag
     2041 ccgcgccccc gcccccatgt acgccacgaa ccgggcgccg gtcatgcggg ccgcccgctc
     2101 gaagtcgagg atgccgagcg ccgcaccgag gtcccagtgc gccttcgcct cgaaggagaa
     2161 cttcggcagc gtcccccagc ggcgcacttc gacgttgtcg tcgctcgtcc tgcccgctgg
     2221 caccgacggg tgcggcaggt tcgggacgtt gagcaggagc gcctcgagcc cttcgtccgc
     2281 ctcgtcggag cgcgcgtcca gctccttgat gcgatccccc gcgccgcgca tctcgtcgat
     2341 gagggcggcg gcgtccttcc cttccttctt gagacgcgcg atgtcgccgg agacggcgtt
     2401 gcggcgggcc ttgaggatct cgacctcgtg gagggccttg cgccgctcgg agtcgaggcg
     2461 acggatcgcc tcgatgtcca cggcggcgcc gcgcttcttc atcgccgttt ccacctcgtc
     2521 gaggtgatcc ctgacccacg tcctgtcgag catcgtgcgg agtctaaact atgatgccgc
     2581 gatggcgcgc acggccaagg cggactacca gcggagcctc aaggtcacca ccctgccgct
     2641 ccccgacccc gacgccgcgg cccgtaccgc cctgcacgtc ctcgacgccc ccgacgcggc
     2701 cggccggctc gccgccggac agcggctcct cgacctcctc gcggcgcaga cttcgatctc
     2761 gaccccggag ctggtcgtcc ccgactcccc gcagccgcac cgccgatcgg gcgggcgcat
     2821 cgtctattcc ctgcaggggg actaccggcg gcgccagcca tcgaaggacg acccggccgt
     2881 cgcccgcggc ggcaagccgc tcggccgcat ccgggtgccg caccgcactc ctgcgcgcgg
     2941 cgacgtcgtc cggccgaccg ctttcctgca caccctcctg cacgagttct gccaccacca
     3001 cgacgccgag tcgctcgggc tcttccgctc gttccacacc gccggcttct acgctcggct
     3061 gcgcgacctg cgttcgcagg tggagccggt cgtgctcgag ccgccgaggc gcaccccaga
     3121 gcgccgccgg aaatcccgcg gtccggccgc accgacgaag gcggctccgc ccctgcagct
     3181 cagcctctgg tccgcgctgc gcgccatctt caagcgctcg tgaccgtgcg gtacgggcct
     3241 ctccgacccc caggacatat aattcgcctt cgatgagccc gcgcgacttc cggacccggc
     3301 ccgacgtgtc gacccaggcc tcgcagccgc ggccgccgcg cgtccgcaag gcggtcttcc
     3361 ccgccgccgg gctcggcacc cgcttcctgc cggcgaccaa ggcgctcccc aaggagatgc
     3421 tgccgctggt ggacaagccg gtcatccagt acgtcatcga ggaggcggtc gactccggca
     3481 tcgagtcgat catcatcgtc accggccgcg gcaagagcgc catcgaggac cacttcgaca
     3541 tctccttcga gctggagtcg ctcctggagc gtcgcggcaa gcacgacctg ctgcgcgagg
     3601 tgcggacgat ctccgatctc atcaatgtct cgtacgtgcg gcagaaagag ccgctcggtc
     3661 tcggccacgc catcctcgtg gcgcgcgacc tcgtggggcc ggagccgttc gccgtcttcc
     3721 tcggcgacga catcatccac gcgccggtcc cggtcatgca gcagatgatc gacgtgcatg
     3781 cgcgcaccgg ctcgtcggtc atcggcgtcg agcgggtggc gcgcgagcgc agccgcgact
     3841 acggcatggt ggcggtggac gagcccggcg cggggcgtac gtatcgcatc accgacctgg
     3901 tggagaagcc gcctccggag gaggcgccgt ccgatctggc gatcatcggg cgctacatcc
     3961 tgacgccgga gatcttcccg cacctcgaga ggaccggcgc cgggcgcggc ggggagatcc
     4021 agctcaccga cgggctgcgc tcgctcctga aggagcagcc gatcaccgcc tacgccttcg
     4081 aggggatccg ctacgacacc ggcgacaagc tcggtttcct gaaggcgacc gtcgagttcg
     4141 ccctgcgccg tcccgacctc ggcggtccgt tccgggcgta cctcgagcag ttgtcgctcg
     4201 acggcgacgc ggtgcgcccc gtcgcgaagc cggacgtcgc gccgcgtgcc gcccggccgg
     4261 cccg
//
LOCUS       DASRIQ010000339         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3255691, whole genome shotgun sequence.
ACCESSION   DASRIQ010000339 DASRIQ010000000
VERSION     DASRIQ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3255691"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFD06_08060"
     CDS             complement(<1..947)
                     /locus_tag="VFD06_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZN03531.1"
                     /translation="MDQRSTSSRIETLAVHGGERRPGPEGSIVFPIYQATVFTYDDDA
                     GYHDIKYIRLNTTPSQKYLHDKLAALEGAEAALATSSGMAAVTCCLHTFLKAGDHLIA
                     GDTLYGGTHTFLTEHAPALGWQVTFVDPGNPETFKPALRPNTKAMLVETITNPLMRVA
                     RHKEVVRFAQEAGITTIIDNTFASPVNFRPLALGYDLAFHSATKYLNGHSDLVAGCVV
                     GRKALIEKVRASLNHYGGTLDPHAGFLLARGMKTLALRVRQQGANALALAKSLAAHPK
                     VEGVNYPGLETHPDHKYARELLNGFGGMLSIRLKGGVPAA"
     gene            1124..2614
                     /locus_tag="VFD06_08065"
     CDS             1124..2614
                     /locus_tag="VFD06_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03532.1"
                     /translation="MDERASVGMFGGVVGRLVRDATSPGTAFTGFVLGVVLLTSAFVC
                     RMAQADLLGQLLAQIVMAAALARFALNGYAGESRGTILSTAGGTWHQVLMVAGRYLAL
                     QLVWVVPVFLLGWSAFASVLRPETNTVLLAPYGGSTMPPAATNGAATLLPILGVLSSG
                     AFLWSIGLMFFGMLLLPPVFLIVAVRSEKFGDVVSPAIWSATFGGRLGDLYTLYAVQA
                     GGMMMGMVLMAPPVLIGFAAGSGLGVLMLVVAMGFLAGLTVTLMGRLCGFFAFGDESP
                     AQPAHSRAVDAEPAPVTDSAGSDSAAEDESPLPPLPDAAERAAAARQRFETDPDAAIA
                     ECEALCAQHAPSPQVLHALALMLHAACRIEDGADVARAAITLSLKRGHTTLAAEVFVA
                     FWKQAQALGLTGEQIDLVATTLLKGGDLAHATTAFGIALRFDHGDRKAIKGLMQIASH
                     MLHREARPKDAARIYTFLLQYASSSPLAGDMRQGLAEAEARLKRAA"
     gene            2697..>3275
                     /locus_tag="VFD06_08070"
     CDS             2697..>3275
                     /locus_tag="VFD06_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03533.1"
                     /translation="MAAVLVAAMAAACARSSEEERTARSTGREGAAKPAAGEAAPDAA
                     AGSTSLPGTGRRIPPLTKGEGTRRMGELLQSLNRAAKPGVNPFLNAERAAQIRDTMPR
                     MADPLDRMLARTQMADELLRAGRVREAIRTVEPLLTPTSAEARYAPPPVQTLEFLGLA
                     SLRLGETDNCIRDHSAESCLLPIRGGGVHRRPE"
BASE COUNT          436 a         1161 c         1158 g          520 t
ORIGIN      
        1 gccgccggca cgcccccctt cagccggatc gacagcatcc cgccgaatcc gttcaagagc
       61 tcgcgggcgt acttgtggtc cggatgcgtc tcgagaccgg ggtagttgac tccctcgacc
      121 ttgggatgcg ccgccagcga cttcgccagc gcgagggcgt tcgccccctg ctgccgtacc
      181 cgcagcgcga gcgtcttcat gccgcgcgcc agcaggaagc cggcgtgcgg gtcgagggtg
      241 ccgccgtagt ggttgagcga cgcgcgcacc ttttcgatga gcgccttcct gccgacgacg
      301 cagccggcga cgaggtccga gtgcccgttg aggtacttcg tcgcgctgtg gaacgccagg
      361 tcgtagccga gcgccagcgg ccggaagttc accggcgagg cgaaggtgtt gtcgatgatc
      421 gtcgtgatcc ccgcctcctg cgcgaagcgg acgacctcct tgtgccgcgc cacgcgcatc
      481 agcgggttgg tgatcgtctc gaccagcatc gccttggtgt tgggccggag cgccggcttg
      541 aacgtctccg ggttccccgg atcgacgaac gtcacctgcc agccgagggc cggcgcgtgc
      601 tcggtcagga aggtgtgggt gccgccgtac agcgtgtcgc cggcgatcag gtgatccccc
      661 gccttgagga acgtgtgcag gcagcacgtc accgccgcca tgccggacga cgtggccagc
      721 gccgcctccg ccccttcgag cgccgccagc ttgtcgtgga ggtacttctg cgacggcgtc
      781 gtgttcagcc ggatgtactt gatgtcgtga tatcccgcat cgtcgtcgta ggtgaagacg
      841 gtcgcctggt agatcgggaa gacgatcgat ccttcggggc cggggcgacg ctcgccgccg
      901 tgcaccgcga gggtctcgat ccgggaagag gtcgagcgct ggtccatgga gggcctccag
      961 gttgtccggg gggtggcgcc ggaatcggaa tgataccgcg aggcgggagg ggtcacgaaa
     1021 gccgggaaca tcttcgtgat cggacatcgg acggccccgc gcatcgctgg cttccccggg
     1081 atcccggcac tacatataga tgccggattc ccggagtcga acgatggacg agcgagcgag
     1141 cgtcgggatg ttcggagggg tggtcggtcg cctggtgcgc gacgcgacct cgcccgggac
     1201 cgccttcacc gggttcgtcc tcggcgtcgt cctcctgacg tccgccttcg tctgccgcat
     1261 ggcgcaggcg gatctcctcg gtcagctctt ggctcagatc gtcatggcgg cggcgctggc
     1321 ccgcttcgcc ctcaacggct acgccggcga gtcccgcggc acgatcctct cgaccgcggg
     1381 cggaacctgg caccaggtgc tcatggtggc ggggcgctac ctggcgctgc agctcgtgtg
     1441 ggtcgtcccg gtgttcctgc tcgggtggag cgccttcgcc tcggtgctgc gccccgagac
     1501 caacaccgtc ctcctcgccc cctacggcgg ctcgacgatg ccgcctgccg ccacgaacgg
     1561 cgccgcgacg ctcctgccga tcctcggcgt cctgagctcg ggggcgttcc tgtggtcgat
     1621 cggcctgatg ttcttcggga tgctgctcct cccgccggtg ttcctgatcg tcgcggtccg
     1681 ctccgagaag ttcggcgacg tcgtctcgcc ggcgatctgg agcgcgacgt tcggcgggcg
     1741 gctcggcgac ctctacaccc tctacgccgt gcaggcgggg gggatgatga tggggatggt
     1801 cctgatggcg ccgccggtcc tcatcggctt cgcggccggt tccgggctgg gcgtcctgat
     1861 gctcgtcgtc gcgatgggct ttctcgccgg gctgaccgtc accctcatgg ggcggctgtg
     1921 cggcttcttc gccttcggcg acgagtcgcc ggcgcagccg gcgcactcgc gagccgtcga
     1981 tgctgagccc gccccggtga cggactccgc cggttcggat tccgccgcgg aagacgagtc
     2041 gccgctgccg ccgctccccg acgccgccga gcgggccgcg gcggcgcggc agcgcttcga
     2101 gaccgacccc gacgccgcca tcgccgagtg cgaagccctc tgcgcgcagc atgccccgag
     2161 cccccaggtg ctgcacgccc tcgccctgat gctccacgcc gcctgccgga tcgaggacgg
     2221 cgccgacgtg gcgcgcgcgg cgatcaccct gagcctcaag cgcggccaca ccactctcgc
     2281 ggcggaagtg ttcgtggcgt tctggaaaca ggcgcaagcg ctcggcctca ccggcgagca
     2341 gatcgatctc gtcgcgacca ccctgctcaa ggggggggac ctggcgcatg cgacgaccgc
     2401 cttcggcatt gcgctccgct tcgaccacgg cgatcgcaag gcgatcaaag ggttgatgca
     2461 gatcgccagc cacatgctgc accgcgaggc gaggccgaag gacgcggctc gcatctacac
     2521 ctttctcctg cagtacgcgt cgagctctcc cctcgccggg gacatgaggc agggtctcgc
     2581 cgaggcggag gcccgtctga aacgggccgc ctgatcggcc ccggccgggg ccggtgcata
     2641 atgcgcccgt gagtttcgcc gactcactcc cgaggtggca tgcctggcgt ggcctgatgg
     2701 cagcggtcct cgtcgccgcc atggctgccg catgcgcccg ctcctccgaa gaggagcgga
     2761 ctgcccgatc gaccggcagg gaaggcgcag cgaagccggc cgcgggcgag gccgcgccgg
     2821 atgcggcggc cggctccacc tccctccccg gcaccggacg acgcatcccc cccttgacga
     2881 aaggggaggg aacgcgccgc atgggggagc tgctccagtc gctcaaccgg gccgcgaagc
     2941 cgggggtgaa cccgttcctc aacgccgagc gggcggcgca gatccgggac acgatgccgc
     3001 ggatggccga cccgctcgac cggatgctgg cgagaacgca gatggcggac gagctcctgc
     3061 gggccggccg cgtccgggag gcgatccgca cggtggagcc gcttctgacg ccgacgtcgg
     3121 ccgaggcacg gtacgcgccg ccgccggtgc agacgctgga gttcctcggg ctcgcgtccc
     3181 tgcgcctcgg cgagaccgac aactgcatcc gcgatcacag cgccgagtcg tgtctcctgc
     3241 cgattcgcgg cggcggcgtg caccgccgcc ccgag
//
LOCUS       DASRIQ010000340        10548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_139470, whole genome shotgun sequence.
ACCESSION   DASRIQ010000340 DASRIQ010000000
VERSION     DASRIQ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10548
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139470"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..328)
                     /locus_tag="VFD06_08075"
                     /pseudo
     CDS             complement(218..328)
                     /locus_tag="VFD06_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
     gene            complement(382..1938)
                     /locus_tag="VFD06_08080"
     CDS             complement(382..1938)
                     /locus_tag="VFD06_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZN03534.1"
                     /translation="MSLQATPSSAGASFTHVPPARLLDQHLRRLSRLRNPVEWRVARM
                     LERLDRGSGYLDLGFARLSDYARERLGLTARRVQTLLGLARRLGPLPRVAAAYESGVL
                     SQSQAALVAMVATAADQEPWVMRAKSLTVRRLTQEVLEALPERERGVRAEVAGLPDDG
                     ATAIVCDEEDAGENVGWTGPARLRPLWDEALELARRSGGADELAEGKRTVLDGAEPDG
                     TDLFEAIRGAIEAEASSGSEADRREASLPDAVIEGLNDTPRLLDLRLRKLVALRQKLA
                     WHQGRLLRVMAARRLYRDLGFLSFSCYCQERAGLGARRAWQLIALDRRLVRLPRLAAA
                     YRTGEVSWVRASAIAGIATEATEEDWIRVARSVTVRRLRAEVWLSTEGVRPPGLASDG
                     RVQLSAPLAGERILQTFVAGWRVRQTAAWRRRYRIFERDGWRCRVPGCSSRAHLQVHH
                     VVFRSHGGGEDDGNLATLCATHHLRGLHQGRLRCHALPDGLLAWEFAPDPVDGALARY
                     VEDVSWEAAR"
     gene            complement(2105..2398)
                     /locus_tag="VFD06_08085"
     CDS             complement(2105..2398)
                     /locus_tag="VFD06_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03535.1"
                     /translation="MRVLLDECLPRKLKFAFPEHEVRSAQQEGWGGLRNGALLPIAIV
                     AMVVPSNRLGDLLPMVRQVRAALIRAKPGAVTRVGDIAVREHVPGYRFSRSAA"
     gene            complement(2379..2606)
                     /locus_tag="VFD06_08090"
     CDS             complement(2379..2606)
                     /locus_tag="VFD06_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HZN03536.1"
                     /translation="MTSKPPVVHSDPGILGGTPVFVGTRVPFQTLLDYLESGDSLDEF
                     LDDFPTVTREQAIAALEQAKEALLAHACPPR"
     gene            complement(2603..3022)
                     /locus_tag="VFD06_08095"
     CDS             complement(2603..3022)
                     /locus_tag="VFD06_08095"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HZN03537.1"
                     /translation="MQRDELQDRMTERRAVEAVVWGMPAVNFELMVRAFEEMHGGWNQ
                     VVYWSRLADWKNQTLTPNPDTVYLMPFYNTKDAGPMVLEIPPAGAGESITGSGSTAPR
                     RRCSTRPGGWGTSRRAMGSAWRGRRTRYTPVQLRRHP"
     gene            complement(3038..3457)
                     /locus_tag="VFD06_08100"
     CDS             complement(3038..3457)
                     /locus_tag="VFD06_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZN03538.1"
                     /translation="MADWVDIPRLTKALPGCVRSGPREWRIKDQLIAWERPLRPADRA
                     ALGADAPTGPILAVYAPLEIKEVLLAARPEVYFTTPHFDGWPAILIRLRAIGVNELRE
                     RLRRAWLERAPKKLIAEVEGAKPAARRRPARRKVSRP"
     gene            complement(3515..3742)
                     /locus_tag="VFD06_08105"
     CDS             complement(3515..3742)
                     /locus_tag="VFD06_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HZN03539.1"
                     /translation="MSSKPTVVHSDPNILGGTPVFVGTRVPVQNLIDYLEAGDSLEDF
                     LNSFPSVTREQAIAALELAREALVAHAHSSR"
     gene            complement(3822..4160)
                     /locus_tag="VFD06_08110"
     CDS             complement(3822..4160)
                     /locus_tag="VFD06_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002785377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HZN03540.1"
                     /translation="MRDSAKTRANLLKHGGSGARMKKEYDFSGGKRGAYLKAPGKSRI
                     TIYLDNDILEKFRERGDAAGRGYQTMINDALRAALGDEAQPINAQTLRRILREEIPKY
                     QVARKSRKRS"
     gene            complement(4398..10349)
                     /locus_tag="VFD06_08115"
     CDS             complement(4398..10349)
                     /locus_tag="VFD06_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03541.1"
                     /translation="MAWAPPSLAVVTGMVMDNENGRAIVFDAETGLVTAFVPIGPNFR
                     GTGDCLVLGDRGPAFATDFDSRVWAFDLESSPPRLATPPNPIRISNPGEDLAISADHR
                     FLVVCDGSGPAPVSVIDLATRREVAAFDLGSDCDAVDVCDDGSVLVSSITRQTIRRLT
                     LGPDGSLTDTGESVAVIAPMNVDCAPGSGSGVVANFFPAGLQSFTIPGLAAVDTRPIE
                     DEDNIAQSVLIHPAGNRVYARAGHSLVAYDFDPSTGHIGPAPAFRAPNRSLGGFFGME
                     QIALDPAGERLYVPQSYPAEIEIRSASTGDLLGSIALPEFSQPTGVCLATEVDGDDDG
                     LSDAREAALGTDADNPDSDGDGLLDGFEVRFGLQPLNGADGGQDADADGLDNLGEQAA
                     RTDPTVADSDGDGLSDGQEVLVWSTDPVNPDSDGDRLQDGAEVSPHGSDPRNPDTDGG
                     GVRDGVEVARDDTNPTDPVDDRPAGRAMLMDADQAQAVIFDRDTEAVVGVVQELYGQA
                     VGDCHILEDGSQAFATTFSWEIHAINLTVTPPVLAPPPNPIPIDNPGEDLAPTVDGRY
                     LVVCDGSSIDPVVVVEVATRTQVDALDLGTDCTSVDVCADGSVLVTSATTGVVRRLEI
                     DAAGHLTDVGVRFNADRPSNITCAPDGRSGIVLSGLPSPAIISFTVPGLAVVERRDTP
                     AAPVALAFAPDGDRVFVRRATNDTGSIEAYGYDRHTGHLDPQPEWTAPVSRARRFFGI
                     DTITVDPSGTELHVTEFGALSILDTGSGRVLSSILDAAIENPTGVCLRQPDRDHDGLT
                     DDLERRLGTDPDDPDSDGDGVPDGYESHHDQDPLSPGDGTTDPDGDGLDNGAEAAART
                     DPANPDTDHDGLSDGEEVLTYATDPRDGDMDGDHLADGEEVHARGTDPRNPDSDGGGA
                     LDGNEVALDASDPLDPADDLAAGRVLFTNYYNGYRATVVDQETSVVLAALTQPTSSGY
                     DCVIPPDGGEGFIPIVNGLRVVDLESTPPAFAASPNPIPTSIYANDVAASPDGKYIVS
                     CSTTGFMTSHGISVVDREARVEVGVSNLVPSCRAIDVCPDGSVLVVSSDGSRITRLML
                     GDDGALTDPQEFVEGGLPTTQWTNVTCAPAGEYVLALRSDQTLRSLRLRGLEPVDVLA
                     LPGGASDPVMQITGLRATIFMKLGGPVRIAAYTIDLATGRFDADPLFVRTIFNIDGYG
                     TLTAEALALDRRGELLYVLHQGVLKIYGTHDGSFQGTIAGAESGQVFAVCAARAGDSD
                     GDGLADDEESRLGTDRRSADTDGDGLLDGFEARNGLDPRSPGDAHDDPDADGLDNLGE
                     QGAGTLPRVPDTDADGLNDGQEVLVAGTDPLDPDTDDDGVLDGADPCPREVPNDPDGD
                     GVCQSVDICPGVPDTAQTDTDADGWGDACDLCPRVSDPAQAESVACFAPITGGGACLQ
                     TTIDMVRAPGESGAIDGAILIHHVGGTPPDAIEFTVLDTACTTGDVFEFELNGVPVGR
                     IEADPTLSCDCQAPQQSFRFEDAAVLAGAWLPSRDNVLRIRKIGNQSGLAWIRLTAWR
                     GVVATETCVFDHGGGDCDVTDLCAAGYVIEGFETTHTFLDPFLAAGAPVVEAPFLGGA
                     LPGTIDLAGIAAGDYLLCARSDVSLPHRMACVPMTLQGQARLILDGAGCGAGPTAAIR
                     GGGASECSSPDGATVLLDGTASIDPDDDIVLYEWFEDYGEASERLVAAGAMTSVTLAL
                     GAHAITLKVTDSGGLTGLAAAPFTVVDTTAPVLSVGLAPALLWPPAHQMVQVNATVGA
                     VDLCGAASFVLTSLQSSEPDDLAGGADGQTVNDVQDALPGTADVAFRLRAERRSDSPG
                     RTYSATYQARDASGNVTQVVAVVLVPHDRHGAVDPIDLRAGVNPGGTVLTWSDAAGLE
                     RYDVVRGDLHRVRELPDVIDLGPLICIESRSADRSTAGNEDADVPAVGEGFFYLIEYD
                     DGVIRTLGSDTTAKPRLPGAGGTGC"
BASE COUNT         1637 a         3732 c         3562 g         1617 t
ORIGIN      
        1 acgagaagtg ccgcggtcgc gccgatggcg gcgccgccga ggaatagcag gacggacttg
       61 ctcgcaacgc tgtgactcat ggagggtctc ctcccttcct ggattggggt gcgcattcga
      121 gcaagcaaga cacatgccgg ataagccacg ctccggcatg ctgcaagcgc tattaatcgg
      181 ctcgcatggc gatcaccggg tccgtgtccg cgaccgaggc ggtccagccg ccgaacttga
      241 acccgccggt ggggtcttcc tccggcccgc ccggggcctg catcacgcca tccatcgaaa
      301 ccatcgcgcc gacgatcact tttctcatag gagtctcctt ggctcgatcc taggacactc
      361 tcatgacgcc acccagcggc ttcatcgtgc ggcttcccag ctcacgtctt cgacgtagcg
      421 ggccagcgcg ccgtcgaccg gatccggcgc gaactcccac gccagcaggc catccggcag
      481 agcgtggcag cgcaaccggc cctggtggag gccacgcaga tggtgcgtcg cgcagagggt
      541 cgcgagattg ccgtcgtcct cgccgccgcc gtgcgagcgg aacaccacgt ggtgcacctg
      601 gagatgggcg cgcgacgagc agcccggcac ccggcagcgc cagccgtccc gctcgaagat
      661 gcggtaacgg cgccgccacg cggccgtctg ccgcacgcgc cagcccgcga caaacgtttg
      721 caggatccgt tcgcccgcca gtggcgcaga caattgcact cggccgtcgg aggcaagccc
      781 gggcgggcgc acgccctccg tcgacagcca gacctccgcg cgcagccggc gcacggtcac
      841 cgatcgcgcg acacgaatcc agtcctcttc cgtcgcttcg gtcgcgatgc cggcgatggc
      901 cgacgcgcgc acccaggaca cctcccccgt gcggtaggcc gccgcgagcc gtggcagccg
      961 cacgagccgc cggtcgagcg cgatgagctg ccaggcgcgc cgcgctccca ggccggcccg
     1021 ctcctggcaa tagcagctga aggaaaggaa gccgaggtcg cgatagaggc gccgcgccgc
     1081 catgacgcgc agcagccgac cctggtgcca ggcgagtttc tgccgcaacg ccacgagctt
     1141 gcgcagccgc agatcgagca ggcgcggcgt gtcgttcagc ccttcgatga ccgcgtccgg
     1201 gagggacgcc tctcgccggt cggcttcgga gccgctcgag gcttccgcct cgatggcgcc
     1261 gcgaatcgcc tcgaacaggt cggtcccgtc cggttcggcc ccgtcgagga ccgtccgctt
     1321 cccttccgcg agctcgtccg cgccgccgct tcggcgcgcg agctcgagcg cctcatccca
     1381 gagagggcgc aacctggccg gtcccgtcca tcccacattc tcgcccgcgt cctcctcatc
     1441 acagacaatg gccgtcgctc catcgtcagg aagaccggcc acctcggccc gcaccccgcg
     1501 ctcccgctcc ggcagcgcct ccaggacctc ctgcgtcagc cgccggaccg tgagcgactt
     1561 cgctcgcatg acccacggct cctgatcggc ggcggtcgcc accatggcga ccagcgccgc
     1621 ctgcgattgc gacaggacgc cggactcgta ggcggcggcc acgcgcggca gcggcccgag
     1681 gcggcgcgcc agccccagga gcgtctgcac ccgccgcgcc gtcagaccca ggcgctccct
     1741 cgcgtagtcg gacagacgcg cgaatccgag atcgaggtaa cccgagccgc ggtccaggcg
     1801 ttcgagcatg cgtgccacgc gccactccac cgggttgcgc aggcgcgaca gccgccgcag
     1861 atgctggtcg agcagccgcg ccggaggcac atgggtgaac gaggccccgg cggacgacgg
     1921 cgttgcctgc agcgacacgc ggccccctct ccacgtgacg tggacgcgga agccgccacg
     1981 aaggtatgca tactgtgcat accatggcgc cggtggtcaa gcgattcttt cggtggactg
     2041 cgacgaagaa cgggcggcgc tgccagcctg gtagcccaca aggaggcgac cgacgcgcgc
     2101 tgattcaggc cgcgctgcgg gagaaccgat atcccggaac gtgctcgcgt acggcgatgt
     2161 cgccgacgcg ggtcacggcc ccaggcttcg ctcgaatcag cgccgcgcgc acctgcctca
     2221 ccattggcag caagtcaccc agccggttgc tcggcacgac catggcaacg atcgcgatcg
     2281 gaagcagcgc gccgttgcgc agccctcccc atccctcctg ttgcgcgctt cggacttcgt
     2341 gctcgggaaa cgcgaacttc agcttgcgcg gaaggcactc atcgaggagg acacgcatgg
     2401 gcgagcaacg cctccttcgc ctgctcgaga gccgcaatcg cctgctctcg ggtcacagtc
     2461 gggaaatcgt ccaggaattc atccagcgaa tcgccgctct caaggtaatc gagcagggtc
     2521 tggaacggaa ctcgcgtgcc cacgaagaca ggtgtgcccc cgagaatccc tggatcgctg
     2581 tgaacaacgg gaggcttgga cgtcatggat gtcgcctcag ttggaccgga gtatagcgcg
     2641 ttcgccggcc gcgccacgcg ctacccatcg ctcttctcga tgtcccccag ccgccaggtc
     2701 ttgtcgaaca gcgtcttctc ggggccgtag aaccgctgcc ggtaatcgac tcccccgcgc
     2761 cggcgggcgg gatctccagc accatcggac ccgcgtcctt ggtgttgtag aagggcatga
     2821 ggtagacggt gtcgggattg ggcgtcagcg tctggttctt ccagtccgcc aggcgcgacc
     2881 agtagacgac ctggttccac cctccgtgca tctcctcgaa ggcgcgcacc atcaactcga
     2941 agttgaccgc cggcatcccc cagaccaccg cctcgaccgc gcgccgctcc gtcatgcggt
     3001 cctggagctc gtcccgctgc acctcgcgct tctcacgtca gggcctcgag actttgcggc
     3061 gcgcgggcct gcggcgcgcg gccggcttgg cgccctcgac ctcggcgatg agcttcttcg
     3121 gagcccgctc cagccacgcg cggcgcagcc gctcgcgcag ctcgttgacg ccaatcgccc
     3181 gcaggcggat gaggatcgcc ggccagccgt cgaagtgagg ggtcgtgaag tacacctcgg
     3241 gccgcgccgc caggagcacc tccttgatct cgagaggcgc gtagacggcg aggatcggtc
     3301 ccgtcggagc gtccgcgccg agggcggccc gatcggccgg acgcagcggc cgctcccacg
     3361 cgatgagctg gtccttgatg cgccactccc gcggtcccga ccgcacacac ccggggagcg
     3421 ctttcgtcag acgcgggatg tcgacccagt ccgccatggc tgcatggaga cgcggtgtcc
     3481 cttcagttcg tccgcgaact cggctggaag gcactcatcg agaagaatgc gcatgtgcta
     3541 ccagagcctc acgagccagt tcgagagccg cgatcgcctg ctcccgtgtc acggacggga
     3601 aagagttcag gaaatcctca aggctatcgc cggcttcaag gtaatcgatg aggttctgaa
     3661 ccggcactcg cgtgccgaca aacaccggcg tgcccccgag aatattcggg tcactgtgaa
     3721 caactgtcgg cttgctgctc attcgcactc cggaacgttg aggccagtat gggtcattcg
     3781 gagccctggt cgaatcgatg ccccctccgt agccttcccg ctcacgaccg ctttcgactc
     3841 ttccgtgcga cctggtactt cgggatctct tccctgagaa tgcgccggag ggtctgcgcg
     3901 ttgatcggct gcgcctcgtc tcccaaggcc gcccgcaacg cgtcgttgat catcgtctgg
     3961 tatccgcggc ccgccgcatc accccgctcg cggaacttct ccaggatgtc gttgtcgaga
     4021 tagatggtga tgcgcgattt gcccggtgcc ttgaggtacg cgccacgctt tcccccgctg
     4081 aagtcatact ccttcttcat ccttgctccg ctccctccat gcttgagcag gttcgcgcgg
     4141 gtcttcgccg agtcccgcac cgtctcctct tgtatgcata ctatacgtat ttgcaacggg
     4201 atgctgactg gccagagagt ccttgactcc gcgccggacg aaggcgctcc tcaccctgaa
     4261 ggtctcggac atccgggcca tccgtggaac ggacatgccc gccattcgtt ccgcgggcca
     4321 ccggacctga acgcccgtca cgcaaacacc cgctcccccc tcacctccga ggcgcagcag
     4381 ccacgccgga cgagcgtcta gcagccggtc ccaccggccc cgggcaggcg cggcttcgcc
     4441 gtcgtatcgc tgccgagggt gcggatgaca ccgtcgtcgt actcgatgag atagaagaag
     4501 ccctcgccga ccgcggggac gtcggcatcc tcgttgccgg cggtgctgcg atccgccgac
     4561 cggctctcga tgcagatcag cgggccgaga tcgatgacgt ccggcagctc gcggacgcga
     4621 tgcagatcgc cgcgcaccac gtcgtaccgc tccagccccg ccgcgtcgct ccaggtgaga
     4681 accgtgcccc cggggttcac acccgcgcgc aggtcgatcg gatcgacggc cccgtgccga
     4741 tcgtgcggca ccaggacgac ggccacgacc tgcgtcacat tgcccgacgc gtcgcgcgcc
     4801 tggtaggtgg cgctgtaggt ccggccgggg ctatcggacc ggcgctcggc gcgcaggcgg
     4861 aacgcgacat cggccgtgcc cggcagagcg tcctgcacgt cgttgacggt ctggccgtcg
     4921 gccccgccgg cgagatcgtc cggctcgctg ctttgcagcg acgtgagcac gaacgacgcc
     4981 gctccgcaga gatcgaccgc ccccaccgtt gcgttcacct gcaccatctg gtgcgcgggc
     5041 ggccagagga gcgcgggagc aagcccgacg ctcaggaccg gcgcggtggt gtcgacgacc
     5101 gtgaacggcg ctgcggcgag tccggtcagg ccgccggagt cggtcacctt gagggtgatg
     5161 gcgtgcgcgc cgagcgccag cgtgaccgac gtcatggcgc cggccgccac gagccgctcc
     5221 gaagcctcgc cgtagtcctc gaaccactcg tagagaacga tgtcgtcgtc gggatcgatc
     5281 gacgccgtgc cgtccaggag caccgtggct ccatcaggtg aggagcactc ggacgcgccg
     5341 ccgccgcgaa tcgccgccgt gggaccggcg ccgcagccgg ccccatccag gatcagccgc
     5401 gcctgcccct gcagcgtcat cgggacgcat gccatgcggt gcggcaggct cacgtcgctg
     5461 cgcgcgcaca gcaggtagtc gccggccgcg atcccggcga gatcgatcgt cccgggcaac
     5521 gccccgccga ggaacggcgc ctcgacgacc ggggctccgg ccgccaggaa cggatcgagg
     5581 aaggtgtgcg tggtctcgaa cccctcgatg acgtatccgg cggcgcacag gtcggtcacg
     5641 tcgcagtcgc cgccgccgtg atcgaagacg caggtctcgg tcgccaccac gccgcgccat
     5701 gccgtcagcc ggatccaggc gaggccggat tggtttccga tcttgcggat gcggaggaca
     5761 ttgtcacggc tcgggagcca cgccccggcc agcacggcgg cgtcctcgaa ccggaacgac
     5821 tgctgcggcg cctggcagtc gcagctcagg gtcggatcgg cctcgatccg cccgaccggc
     5881 acgccgttga gctcgaattc gaagacgtca cccgtggtgc aggcggtgtc gagaaccgtg
     5941 aactcgatcg cgtccggcgg cgtgccgcca acgtgatgga tcagaatggc gccgtcgatg
     6001 gcgccgcttt cccccggggc cctgaccatg tcgatcgtcg tctgcagaca ggctcccccg
     6061 ccggtgatgg gagcgaagca ggcgaccgac tccgcctggg ccgggtccga aacgcgcggg
     6121 cagagatcgc aggcatcccc ccatccgtcg gcatccgtgt cggtctgcgc cgtgtccggg
     6181 acgccggggc agatgtcgac gctctggcag acaccatcgc cgtccgggtc gttcggcacc
     6241 tcgcgtggac agggatcggc gccgtcgagg accccgtcgt catccgtgtc gggatcgagc
     6301 gggtcggtgc cggccacgag gacctcctgt ccgtcgttca ggccatcggc gtcggtgtcc
     6361 gggacgcggg gcagcgtccc ggcgccctgc tcaccgaggt tgtcgagccc atccgcgtcc
     6421 gggtcatcat gcgcatcgcc gggcgaccgc ggatcgagac cgttgcgcgc ctcgaaccca
     6481 tcgagcaggc cgtctccgtc cgtgtcggcg ctgcggcgat cggtgcccag ccgcgattct
     6541 tcgtcatcgg ccaggccgtc gccatcgctg tccccggccc gcgcggcgca gacggcgaac
     6601 acctggccgc tttcggcacc ggcgatggtg ccctggaagg agccgtcgtg cgttccgtag
     6661 atcttcagca ccccctggtg caggacgtac agaagctccc cgcggcgatc gagcgccagc
     6721 gcctccgcgg tcagggtgcc gtagccgtcg atgttgaaga tcgtccggac gaacaacgga
     6781 tcggcgtcga agcgtccggt ggcgaggtcg atcgtgtacg cggcgatccg gaccgggccg
     6841 ccaagcttca tgaagatggt cgcccgcagc ccggtgatct gcatgaccgg gtcgctcgcg
     6901 ccgccgggga gcgccaggac atcgaccggt tcgagaccgc gcagccgcaa ggagcgcaat
     6961 gtctggtcgg aacgcagcgc gaggacgtac tcgcccgccg gcgcgcaggt gacattcgtc
     7021 cattgggtgg tcggcaaacc accctcgacg aactcctgtg ggtcggtgag cgccccatca
     7081 tcaccgagca tcagccgggt gatccgggat ccgtcgctgc tcacgaccag gacggagccg
     7141 tccggacaga catcgatggc gcggcacgat gggaccaggt tcgagacacc gacttcgaca
     7201 cgcgcctccc ggtcgacgac cgaaatcccg tgtgacgtca tgaaacccgt cgtggagcag
     7261 ctcacgatgt atttcccgtc cgggctcgcc gccacatcat tcgcgtagat cgaggtgggg
     7321 atcggattcg gcgacgcggc gaaggcgggc ggcgtggact ccagatcgac gacccgcagg
     7381 ccattcacga tcggaatgaa gccctcgccg ccatcgggtg ggatgacgca gtcatatccg
     7441 gaggaggtgg gctgcgtgag ggcggccagg acgacgctgg tctcctgatc gacgacggtg
     7501 gcgcggtagc cgttgtagta gttggtgaac agcacccggc ccgcggcgag atcgtccgcc
     7561 gggtcgagcg ggtcggaggc atcgagcgcg acctcgtttc cgtccagggc acccccgccg
     7621 tcgctgtccg ggttgcgcgg atcggttccg cgagcgtgaa cctcctcgcc gtccgccagg
     7681 tggtctccgt ccatgtcgcc gtcgcgcggg tcggtcgcgt acgtcagaac ctcctccccg
     7741 tcggacagac cgtcgtggtc ggtgtcggga ttcgcggggt cggtgcgcgc cgccgcttcg
     7801 gccccgttgt cgagaccgtc cccatcgggg tcggtcgtcc cgtcgccggg cgacagcgga
     7861 tcctggtcgt ggtgcgactc gtagccgtcg ggaacgccgt caccgtcgct gtcggggtcg
     7921 tccgggtcgg tcccgagacg ccgctcgagg tcatcggtca ggccgtcgtg gtcgcgatcc
     7981 ggctggcgca gacagacgcc ggtggggttc tcgatggcgg cgtccaggat gctggagaga
     8041 acccggccgc tgccggtgtc caggatcgac agcgccccga actcggtgac atggagctcc
     8101 gtcccggagg ggtcgacggt gatcgtgtcg attccgaaga aacgccgcgc gcggctcacc
     8161 ggtgcggtcc attccggctg cggatcgaga tgccccgtgt gccggtcgta cccgtaggcc
     8221 tcgatcgagc cggtgtcgtt cgtggcgcgc cgcacgaaca cgcgatcccc gtccggcgcg
     8281 aaggcgagcg ccacgggggc ggccggcgtg tcgcgccgct cgaccaccgc caggccgggt
     8341 acggtgaagg agatgatggc cggggacggc agccccgaaa ggacgatccc ggatcggccg
     8401 tccggcgcac aggtaatgtt ggacggccgg tcggcgttga agcggacacc gacgtcggtc
     8461 aggtgcccgg cggcgtcgat ctcgaggcgg cgcaccacgc ccgtggtcgc cgaggtcacc
     8521 agcaccgagc cgtcggcgca gacatccacc gaggtgcagt cggtgccgag gtccagggcg
     8581 tcgacctggg tgcgagtcgc gacctcgacg accacgaccg ggtcgatgct gctgccgtcg
     8641 cagaccacaa ggtaccggcc gtcgaccgtc ggagcgagat cctcgccggg gttgtcgatg
     8701 gggatcgggt tgggcggcgg agcaagcacc ggcggcgtca ccgtcaggtt gatagcgtgg
     8761 atttcccagc tgaaggtggt cgcgaacgcc tggctgccgt cctcgaggat gtgacaatcg
     8821 ccgaccgcct ggccgtagag ctcttgcacg acgccgacga ccgcctcggt gtcgcgatcg
     8881 aagatcaccg cctgggcctg gtcggcgtcc atgagcatcg cgcggccggc cggtcggtcg
     8941 tcgaccggat cggtcgggtt ggtgtcgtcg cgtgcgacct cgactccgtc acgcacgccg
     9001 ccgccgtcgg tgtcggggtt ccgcggatcg gacccgtgcg gcgacacctc ggcgccatcc
     9061 tgcagccggt caccgtcgct gtccgggttc acggggtcgg tgctccagac cagcacctcc
     9121 tggccgtccg acaggccgtc gccgtcggag tcggccacgg tcgggtcggt gcgcgccgcc
     9181 tgttcgccga gattgtccag cccgtccgcg tcggcatcct ggccgccatc cgccccgttg
     9241 agcggctgga gaccgaagcg cacctcgaag ccgtcgagca ggccgtcgcc gtcactgtcc
     9301 ggattgtccg catccgtccc cagagccgct tcgcgcgcat ccgaaagacc gtcgtcgtcg
     9361 ccgtcgacct ccgtcgccag gcagaccccg gtcggctggc tgaactccgg gagcgcgatc
     9421 gacccgagca ggtcccccgt ggacgcgctt cggatctcga tctcggcggg atacgactgc
     9481 ggcacgtaga gcctctcccc ggccggatcg agggcgatct gctccatccc gaagaagccg
     9541 cccagtgacc ggttgggggc cctgaacgca ggggcgggcc cgatgtgacc ggtcgacgga
     9601 tcgaagtcgt acgccacgag cgaatgcccg gcgcgggcat acaccctgtt cccggcggga
     9661 tggatcagga ccgactgcgc aatgttgtcc tcgtcttcga tgggccgggt gtccacggcg
     9721 gcgagacctg gaatcgtgaa cgactggagg ccggcaggaa agaagttcgc cacgacaccc
     9781 gacccggagc ccggggcgca gtcgacgttc atcggcgcga tgaccgcaac gctctcgccg
     9841 gtgtcggtca ggctcccatc ggggcccagg gtcaggcggc ggatggtctg cctggtgatc
     9901 gacgagacca gcaccgaccc gtcgtcgcag acatcgacgg cgtcgcaatc ggagccgagg
     9961 tcgaacgcgg cgacctcgcg ccgggtggcg agatcgatca ccgacaccgg cgccggaccg
    10021 ctgccgtcac agaccaccag gaaccggtgg tcggcgctga tcgccagatc ttccccgggg
    10081 ttcgagatcc ggatcgggtt ggggggcgtc gccagccgcg gcggcgagga ttcgagatcg
    10141 aaggcccaga cccgggagtc gaaatcggtg gcgaaggcgg ggccgcggtc accgaggacg
    10201 agacagtcgc cggtcccccg aaaattcggc ccgatgggga cgaacgccgt caccagcccg
    10261 gtctcggcat cgaagacgat cgcccggccg ttctcgttgt ccatcaccat gccggtgacg
    10321 accgcgagcg aaggcggcgc ccatgccatc tgggccagca gaatgagact gagtcggcct
    10381 gcgactcgcc gggcacgctt ccccatggca ctccccacct gcccattccg ctcgagcggc
    10441 atggtcgacc cccctttatt gaggagatca ccaacggcgc cgaaaagccg acatgcgaga
    10501 cttttcactt gttgcattac ggcgacggca gggtatatgg tcggggca
//
LOCUS       DASRIQ010000341         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2463813, whole genome shotgun sequence.
ACCESSION   DASRIQ010000341 DASRIQ010000000
VERSION     DASRIQ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2463813"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFD06_08120"
     CDS             <1..366
                     /locus_tag="VFD06_08120"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZN03542.1"
                     /translation="CNQILVGVTLLGVSEAIAFARKSGLDPHVMLRAVESGAAGSWQL
                     SNLGPRILKDDVAPGFMVDLMLKDLRLVSETAEAAGASLPATSVVERLFDAAREQGWG
                     REGTQVLGRVVAGLEREGA"
     gene            363..1094
                     /locus_tag="VFD06_08125"
     CDS             363..1094
                     /locus_tag="VFD06_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZN03543.1"
                     /translation="MKPRPMIRGARPLRKDRAPSGAASPTASETAIARRNLLRADADL
                     TGAVDAAIARAARLAGPQLVCRAGCSECCIGLFPITRLDAWRLREGLTTLEQSDPERA
                     RRVVERARAAVSTMREEFPGEAGRGLLNGDEEAEDRFLERHADRPCPVLDPATQTCDL
                     YAHRPVSCRTYGPPVKFNGKDLPPCRLCFTTAEPQAIEGCRVAPDRHGLERAILQRMK
                     RDDGETRETLIAWAVLPAGTDPEKA"
     gene            1174..2307
                     /locus_tag="VFD06_08130"
     CDS             1174..2307
                     /locus_tag="VFD06_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN03544.1"
                     /translation="MSRLRVLQVRLPGTLMRAVLACLACAALVAGFALGTGVPPASAQ
                     DFPPCPDRDGDHYADCTGGACDSTGLQCGDCNDDDPRTSPGQPELCDCEDTNCNGLPD
                     DNIDCGEGQPWNCRPDNCPFAYNPRQDDTDSDGVGDACDNCPVWPNPDQANFDGDLLG
                     DACDSCPAIADEGFDIDQDGVGDACDNCRTDPNPSQADADGDGFGDTCDPCDVVSDPG
                     QQDTDQDGFGDLCDVCPFVPDPAQFDTDGDGVGDACDNCPLIPNPDQHDQDGNGAGDV
                     CDDSGLLVLVFIDFKSPAGKGSGLVTWTIPVEYDVIGYNVVIWDRNTRIQINPALIPC
                     QQCGTGQEATYAVIVPKHKDGRDVYVEQVSHDRVQSYGPAVRR"
     gene            2341..3210
                     /locus_tag="VFD06_08135"
     CDS             2341..3210
                     /locus_tag="VFD06_08135"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HZN03545.1"
                     /translation="MIRQRQWWQVSSAAAWLVAAVLLFTPEARGQGLPPCLDADADGY
                     ADCITTTCDSAGLQCGDCNDVEARMHPGLEEACDCLDNDCNGLRDELPLCEILDADGD
                     SLVCENENCPFVYNPSQMDSDGDGVGDVCDNCPSVANADQEDADGDGDGDACDNCPDV
                     ANSDQLDPDGDGLGLACDPCPNDPDPQAVCADVFEVWIDFQSPAGKGSGLVTWKPLPE
                     VGVLGYNLVTFDNNGVRTQFNPVLIPCQACDTGLIPTYAYIVPKHKSGRNLYVELVRL
                     NGAREIYGPAVRR"
     gene            complement(3200..>4212)
                     /gene="trmFO"
                     /locus_tag="VFD06_08140"
     CDS             complement(3200..>4212)
                     /gene="trmFO"
                     /locus_tag="VFD06_08140"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HZN03546.1"
                     /translation="PLIHLRREEVTAIPEEGPCIVATGPLASDAIAAAIARFTGSEYL
                     YFYDAISPIIEADTIDRTRVFGASRYGKGGDDYLNCPLTAEEYDRFYEALLSAAEAPS
                     HDIDRPAHGKTPYFEGCLPIEEMARRGRATLLFGPMKPVGLVDPRTGRQPFAVVQLRQ
                     DTLAGDFFSMVGFQNHLKFGEQQRIFRMIPGLEQAEFPRLGQVHRNSYINAPAILQPT
                     FQARRRRDLFFAGQISGVEGYLESAASGLVAGINAARLAEGLPALMFPATTALGALGR
                     YISGSEPQNYQPTNIAFGLLPPLAQEIRDKRKKKEALVARALRDHDAFIAASLAAPAP
                     IA"
BASE COUNT          670 a         1406 c         1501 g          635 t
ORIGIN      
        1 tgcaatcaga tcctggtcgg ggtgacgctc ctcggtgtgt cggaggcgat cgccttcgcg
       61 cgcaagagcg gcctcgaccc gcacgtcatg ctgagggcgg tcgagtcggg cgccgccggg
      121 tcgtggcagc tgtcgaacct cggaccgcgg atcctgaagg acgacgtcgc gccgggattc
      181 atggtcgacc tgatgctcaa ggatctgcgg ctcgtgtcgg agaccgccga ggcggcggga
      241 gcgagcctcc ccgcgacctc cgtcgtcgag cgcctgttcg acgctgcgcg cgagcagggc
      301 tggggacgcg aggggacgca ggtgctcggc cgggtcgtcg ccgggctgga gcgggaggga
      361 gcatgaagcc tcgaccgatg atccggggag cccggccgtt gcggaaggac cgcgcgccct
      421 ccggggccgc ctcgccgacc gcctccgaga cggcgatcgc ccggcgcaat ctgctgcgcg
      481 ccgacgccga tctgaccggc gccgtggacg ccgcgatcgc ccgggcagcg cggctggcgg
      541 gcccgcagct cgtctgccgt gccgggtgca gcgaatgttg catcgggctg ttcccgatca
      601 cccggctcga cgcgtggcgg ctgcgcgaag gtctgacgac gctcgagcag tcggatccgg
      661 agcgggcccg tcgcgtcgtc gagcgcgccc gggccgcggt ttcgacgatg cgcgaggagt
      721 tccccggcga ggccgggcgc ggactcctga acggcgacga ggaggcggag gatcgcttcc
      781 tcgaacgtca cgccgatcgg ccctgcccgg tcctcgatcc tgcgacgcag acctgcgatc
      841 tctacgcgca ccggccggtg tcgtgccgca cctacggccc tcccgtgaag ttcaacggaa
      901 aggacctccc cccttgccgg ctctgcttca ccaccgccga gccccaggcg atcgaggggt
      961 gccgcgtcgc gccggaccgc cacggcctgg agcgggccat cctgcagcgg atgaagcggg
     1021 acgacgggga gacccgtgag acgctcatcg cctgggcggt cctgccggcc gggaccgacc
     1081 cagagaaggc ttgacacagc cattttccgg ccgtatacga cagtcaaacc accgccgatc
     1141 tccgggagcc cgccgcaccc cgagaagggg accatgtctc gtctgcgtgt gcttcaggtt
     1201 cgattaccgg gcaccctgat gcgcgccgtc ctcgcgtgcc tggcgtgcgc cgccctggtg
     1261 gcgggatttg cgctcgggac gggcgtgccc cccgcgagcg cccaggactt tccgccctgt
     1321 cccgacaggg acggcgacca ttacgccgat tgcaccggtg gggcgtgcga ttcgaccggg
     1381 ctgcagtgcg gcgattgcaa cgacgacgac ccgcggacga gccccgggca gcccgagttg
     1441 tgcgattgcg aggatacgaa ttgcaacggg ctgccggacg acaacatcga ttgtggcgag
     1501 gggcagccct ggaattgccg ccccgacaac tgcccgttcg cctacaaccc gagacaggac
     1561 gacacggaca gcgatggcgt cggggacgcg tgcgacaact gccccgtctg gccgaacccg
     1621 gatcaggcga acttcgacgg cgacctgcta ggagacgcgt gcgattcgtg ccccgccatc
     1681 gcggacgagg ggttcgatat cgaccaggac ggcgtcggcg acgcctgcga caactgccgc
     1741 accgacccca acccctcgca ggccgacgcg gatggggatg gtttcggcga tacctgcgac
     1801 ccgtgcgacg tggtttccga tcccggccag caggacaccg accaggacgg cttcggggat
     1861 ctctgcgatg tctgtccgtt cgtccccgac ccggcgcagt tcgatacgga tggcgacggt
     1921 gtcggtgacg cctgcgacaa ttgcccgctg atccccaacc cggaccagca cgatcaggac
     1981 ggcaacgggg ccggcgatgt gtgcgatgac tctgggctcc tggtcctggt cttcatcgac
     2041 ttcaagagcc cggccggaaa gggctcgggg ctcgtcacct ggaccatccc cgtggagtat
     2101 gacgtcatcg gatacaacgt ggtgatttgg gaccgcaaca cgcgcatcca gatcaacccg
     2161 gcgctgatcc cctgtcagca gtgcggtacc ggtcaagagg ccacctacgc cgtgatcgtc
     2221 ccgaagcaca aagatgggcg tgacgtgtac gtggagcagg tgtcccatga ccgtgtgcaa
     2281 tcgtacggac ccgcggtcag gcggtagatt ccggggagcg cgcccgggga gggcctcatg
     2341 atgatccgac agcgtcagtg gtggcaggtt tcctcggcgg ccgcatggct ggttgcggcg
     2401 gtcctgctct tcacgcccga ggcacgcggg caggggctcc cgccttgcct ggacgccgac
     2461 gccgacggct acgccgactg catcacgacg acgtgcgatt ccgcgggcct ccagtgcggc
     2521 gactgtaacg atgtcgaggc gcggatgcat ccggggctcg aggaagcctg cgactgcctc
     2581 gacaacgact gcaacgggct gcgggacgag ctgcccctgt gcgagatcct cgacgcggac
     2641 ggcgattcgc tcgtgtgcga gaacgagaac tgtcccttcg tctacaaccc gtcgcagatg
     2701 gattccgacg gcgacggcgt cggcgacgtc tgcgacaact gtccgagtgt cgcgaacgcg
     2761 gatcaggagg acgcggacgg agatggggac ggcgacgcgt gcgacaactg ccctgacgtc
     2821 gccaattccg atcaactcga tccggacggc gacggtctgg gcctggcctg cgatccgtgc
     2881 ccgaacgacc cggatccgca ggcggtctgc gcggatgtct tcgaggtgtg gatcgacttc
     2941 cagagcccgg ctggaaaagg gtcgggactc gtcacctgga agccgctgcc ggaggtcggc
     3001 gtcctcgggt acaacctcgt caccttcgac aacaacgggg tccggaccca gttcaacccg
     3061 gtgctgatcc cctgccaggc ctgcgacacg gggctcatcc ccacgtacgc ctacatcgtc
     3121 ccgaagcaca agagcggcag gaatctgtac gtcgagctgg tgcgtctcaa cggcgcacgg
     3181 gagatctacg gcccggcggt caggcgatag gggcgggagc cgccaggctc gcagcgatga
     3241 aggcgtcgtg gtcccggagc gcgcgcgcca cgagcgcctc cttcttcttc cgcttgtcgc
     3301 ggatctcctg agcgagcggc ggcagcaggc cgaaggcgat gttggtcggc tggtagttct
     3361 gaggctcgga cccggagatg tagcggccga gggcgccgag ggcggtcgtc gccgggaaca
     3421 tcagcgccgg cagcccctcc gccagacgtg ccgcgttgat gccggccacg agtccggagg
     3481 cggccgattc gaggtacccc tcgacgccgg agatctgccc cgcgaagaac aggtcgcggc
     3541 gccggcgcgc ctggaacgtc ggctgcagaa tcgccggggc gttgatgtag gagttgcggt
     3601 gcacctggcc gaggcgcggg aactccgcct gctcgaggcc gggaatcatc cggaagatac
     3661 gctgctgctc cccgaacttg agatggttct ggaagccgac catcgagaag aaatcgccgg
     3721 ccagcgtgtc ctggcggagc tgcacgacgg cgaacggctg gcggccggtg cgcggatcga
     3781 cgaggccgac cggcttcatc gggccgaaca gcagcgtcgc gcggccgcgc cgcgccatct
     3841 cctcgatcgg caggcagccc tcgaagtacg gggtcttccc gtgcgccgga cgatcgatgt
     3901 cgtgcgacgg cgcctcggcg gcgctcagga gcgcctcgta gaagcggtcg tactcctcgg
     3961 ccgtcagtgg gcagttgagg tagtcgtccc cgcccttgcc gtagcgcgag gctccgaaga
     4021 cacgcgtccg atcgatggtg tcggcttcga tgatgggcga gatggcgtcg tagaagtaca
     4081 ggtactcgga gccggtgaag cgcgcgatcg ccgcggcgat ggcgtcggac gccagcggcc
     4141 cggtcgccac gatgcacggc ccctcctccg ggatggcggt gacctcttcg cgccgaaggt
     4201 ggatgagcgg ct
//
LOCUS       DASRIQ010000342         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3803730, whole genome shotgun sequence.
ACCESSION   DASRIQ010000342 DASRIQ010000000
VERSION     DASRIQ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3803730"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFD06_08145"
     CDS             complement(<1..327)
                     /locus_tag="VFD06_08145"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HZN03547.1"
                     /translation="MTRHSGDKTASATGLKVWVAMGSDSDLETMQETTKTLESFGIPY
                     QVDVTSAHRTPVETARLASRLEEDGFGVAILGAGHAAHLAGVVAAHTILPVIGVPLPS
                     SALQGLD"
     gene            complement(370..627)
                     /locus_tag="VFD06_08150"
     CDS             complement(370..627)
                     /locus_tag="VFD06_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03548.1"
                     /translation="MPHSSRPSRRIFELLKAYHESRENIRALVDMSRMNDYEKVGFID
                     AWQEEMADFFADNGYCFACNRRLERCGCEDPLRPLAAHRPN"
     gene            complement(765..2054)
                     /gene="purD"
                     /locus_tag="VFD06_08155"
     CDS             complement(765..2054)
                     /gene="purD"
                     /locus_tag="VFD06_08155"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZN03549.1"
                     /translation="MTILVVGGGGREHALAWGVRAGNARRKVLCVPGNAGIARVAECV
                     PGDPTAAAALADLAQSRGVDLTIVGPEAALAAGLVDEFARRGLKAFGASRAAAEIETS
                     KIFAKEFMRRHGIPTAGFEVADSAAAAASILARRGDRPVVVKADGLAAGKGVVVASGR
                     REAEEAVAAMLVERRFGAAGDRILLEDRLSGPEVSFFALCDGETARPLTTCQDYKRLQ
                     DGDRGPNTGGMGGYSPSTLVDAALEREIMERVVAPTVRGLASEGRPYRGVLYVGLMLT
                     SSGLQVLEYNARFGDPEAELIVLRLAGDLVEVMEATLAGRLDEAPLRWHPGGAVCVVL
                     AAAGYPGTPRTGDPISGLDARSDDDALVVFHAATRRGDGGVATAGGRVLTVTARADDL
                     AQARRRAYAEVETIRFHGMQYRKDIAAGAAVESGGRS"
     gene            2075..3358
                     /locus_tag="VFD06_08160"
     CDS             2075..3358
                     /locus_tag="VFD06_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZN03550.1"
                     /translation="MTRPLLPALLIPLALLQLAPIGAPLAADAPAPPTDGTPALVDHP
                     LGPLPQDTPEHEAYAHGHYWLILVWNLFILAVLWAMLQSRFASRVRRFAEGITRRPNV
                     MVMWCVAIFSAVLQIVSLPLAIGTGFLREKRYGFMNQDFGAWMGDRGKQFLVTLCVRL
                     LLVTILYLLMRRFGRGWWVPGAALTIALAIVGIAVTPVFVDPLFNTFTPLQDADLRRE
                     ILDLAHAQGIPAHEVYQMDASRQSEHNNAYVTGLLGTQRIVLYDTLLERFAPREIRSI
                     MGHEMGHYVLNHIWRTVAFLVPLIFVSFLFVDRLARRLIDAHPRWDIRRLEDPASLPV
                     VALLALIVTLATGPAVAAYSRRQESAADRFALEVTRDPEAAASTFVKFGRYDLSEYDA
                     HPLIEALLFSHPSVNNRIRQAQAWARAHPGTTESN"
     gene            complement(3363..4337)
                     /locus_tag="VFD06_08165"
     CDS             complement(3363..4337)
                     /locus_tag="VFD06_08165"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HZN03551.1"
                     /translation="MLISTRVRLRSGWSGGQYSLCRVLLGILLAAHLLRLLPGATWGI
                     RILLAAAVAGTLLLAVGWRDRAAAILVLIVLGLVTVTPALAGVAPAPLDARVLVWPWF
                     LMAHLLVPAAPYGSMAARGRVDPRGDWDMPAWMPWVHRAAFVLLVLRVLGRGAGTADV
                     VAVGLLGLLSFDPGWIAPAGRRAKARGADKHPETLFYDGTCGLCHGAVRFLLAEDRFA
                     RFRFAPLQSRAFRTALPKDERDALPDSLVLKTREGTILTRARAVRHLLASIGGSWRLI
                     AAIARIVPLPLADAAYDAVARVRHRLFRRPDDACPLTPPDLRARFLPG"
     gene            4466..>4720
                     /locus_tag="VFD06_08170"
     CDS             4466..>4720
                     /locus_tag="VFD06_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN03552.1"
                     /translation="MSGGPDAPRRGRGALSNREGRYERTRVETGPDIEPDPDVDPEGE
                     PPPLDTVVTPERTRSILSTNDSPDVGFDQSINPYRGCEHGC"
BASE COUNT          683 a         1753 c         1629 g          655 t
ORIGIN      
        1 gtcgagcccc tggagcgccg acgacgggag cggcacgccg atcaccggca ggatggtatg
       61 cgccgccacg accccggcga gatgggcggc gtgcccggct ccgaggatgg cgacgccgaa
      121 gccgtcctcc tcgagccgcg aggcgagacg cgcggtctcg accggcgtgc ggtgggcgct
      181 cgtgacgtcc acctggtagg ggatgccgaa cgactcgagt gtcttggtcg tttcctgcat
      241 cgtctcgaga tcgctgtcgc tccccatcgc cacccagacc ttgagcccgg tggcggatgc
      301 ggtcttgtcg ccggagtgtc gcgtcacgcc gttcctcctg tcaggaagcg gcgattctaa
      361 cggatttcgt cagttggggc ggtgggcggc gagcggccgg agcgggtcct cgcagccgca
      421 gcgctcgagg cgacggttgc aggcgaagca gtagccgttg tcggcgaaga agtcggccat
      481 ctcctcctgc caggcgtcga tgaaccccac cttctcgtag tcgttcatcc ggctcatgtc
      541 gacgagggcg cggatgtttt cgcgcgactc gtgataggcc ttcagaagct cgaagatgcg
      601 gcgggatggg cgggaactgt ggggcaatcg gcacctctcc aaacgaccgg cgaatcgtac
      661 catcacgact cgtttcgggc cattcgaccc cgccttggcg tgcggagagt cccccgcgtc
      721 cgggggccgg gaatcggcgg gaaccaggtc ctggacgggc ctcttcagga gcggccgccg
      781 gactccacgg cggccccggc cgcgatgtct ttgcggtatt gcattccgtg gaaccggatc
      841 gtctcgacct cggcatacgc ccgccgccga gcctgcgcca gatcgtcggc gcgcgccgtc
      901 accgtcagca cccgcccccc cgccgtcgcg acgcctccgt caccgcggcg tgtcgcggca
      961 tggaacacca cgagggcgtc gtcgtcgctc cgggcgtcga ggccggagat cgggtccccg
     1021 gtgcgcggcg tccccgggta ccccgcggcg gcgaggacga cgcagaccgc gccgcccgga
     1081 tgccagcgca gcggcgcctc gtcgagccgt ccggcgagcg tcgcctccat cacctcgacc
     1141 agatcccccg ccaggcggag cacgatgagc tccgcctccg gatcgccgaa gcgtgcgttg
     1201 tactcgagga cctggagtcc gctcgaggtg agcatcagcc cgacgtagag cacgccgcgg
     1261 taggggcgcc cctcggaggc gaggccgcgc accgtgggcg cgacgacgcg ctccatgatc
     1321 tcccgttcga gcgcggcgtc gacgagggtg gacggggaat agcctcccat cccgccggtg
     1381 ttcggaccgc ggtcgccgtc ctggagccgc ttgtagtcct ggcaggtggt gagcggtcga
     1441 gccgtctcgc cgtcgcacag cgcgaagaag gagacctccg gtccgctcag gcgatcctcg
     1501 agaaggatcc ggtcgccggc cgcgccgaag cgccgctcca cgagcatcgc ggcgaccgcc
     1561 tcctccgcct cgcgacggcc ggacgcgacc acgaccccct tgccggcggc caggccgtcg
     1621 gccttcacga ccacaggacg atctccgcgc cgcgccagga tcgaggcggc cgcggcggcg
     1681 ctgtcggcga cctcgaaccc cgcggtcggg atcccgtgtc ggcgcatgaa ctccttcgcg
     1741 aagatcttgc tcgtctcgat ctccgccgcc gcccggctgg ctccgaacgc cttcaggccg
     1801 cggcgcgcga actcgtccac gagcccggcg gcgagggccg cctccggccc gacgatcgtg
     1861 agatcgacgc cgcgcgactg cgccaggtcg gcgagggcgg cggcggcggt cggatccccc
     1921 gggacgcact cggcgacccg ggcgatcccg gcgttgcccg ggacgcacag gactttccgg
     1981 cgcgcgttcc ccgcgcgcac gccccaggcc agcgcgtgct cacgtccccc gcccccgacc
     2041 accaggatcg tcatcgcggg tattctatcc gtccgtgacg cgcccgctgc ttcccgcgct
     2101 cctgatcccg ctcgcgctcc tgcagctcgc tccgatcggc gcccccctcg ccgcggacgc
     2161 cccggcgcct cccacggacg ggacaccggc gctggtcgat cacccgctcg gtccgctgcc
     2221 tcaggacacg ccggagcacg aggcgtacgc gcacggccac tactggctga ttctcgtctg
     2281 gaacctcttc atcctcgcgg tcctctgggc gatgctgcag tcccgcttcg ccagccgcgt
     2341 gcggcggttc gcggaaggaa tcacccgccg gcccaacgtc atggtcatgt ggtgtgtggc
     2401 gatcttctcg gccgtcctgc agatcgtctc gctgccgctg gcgatcggca ccgggttcct
     2461 gcgcgagaag cgctacggct tcatgaacca ggacttcggc gcctggatgg gggaccgcgg
     2521 caagcagttc ctggtcacgc tgtgcgtccg gctgctcctc gtgaccatcc tctatctgct
     2581 gatgcgccgg ttcgggcgcg gctggtgggt gcccggagcg gcgctgacga tcgcgctcgc
     2641 catcgtcggc atcgccgtca cgccggtctt cgtcgatccg ctgttcaaca ccttcacgcc
     2701 tcttcaggac gcggacctgc gccgcgagat cctcgatctc gcgcacgcgc agggaatccc
     2761 ggcgcacgag gtgtaccaga tggacgcgag ccggcagtcg gagcacaaca acgcctatgt
     2821 caccgggctc ctggggaccc agcggatcgt cctgtacgac acgctcctgg agcgcttcgc
     2881 gccccgcgag atccgctcca tcatgggtca cgagatgggg cactacgtgt tgaaccacat
     2941 ctggaggacg gtggcgttcc tcgtgccgct catcttcgtg agcttcctgt tcgtcgatcg
     3001 cctcgcgcgg cgtctcatcg acgcccaccc acgctgggac atccgccgcc tcgaagaccc
     3061 ggcctccctt ccggtcgtgg cgctgctcgc cctgatcgtc acgctcgcga ccggccccgc
     3121 cgtcgcggcc tacagccggc gccaggaaag cgccgccgac cgcttcgccc tcgaggtcac
     3181 gcgcgatccc gaggcggccg cctcgacctt cgtcaagttc ggccgctacg acctgtcgga
     3241 atacgacgcc cacccgctga tcgaggcgct gctcttcagc cacccgagcg tcaacaaccg
     3301 gatccgccag gcgcaggcct gggcccgggc gcaccccggg acgacggaat cgaactgagg
     3361 cttcagccgg gaaggaagcg ggcgcgcaga tcgggcggcg tcagcgggca ggcgtcgtcg
     3421 gggcgccgga agagacggtg cctcacgcgc gcgaccgcgt cgtaggccgc atcggcgagg
     3481 gggagcggca cgatgcgggc gatcgccgcg atcagcctcc aggagccgcc gatcgacgcg
     3541 aggaggtgtc gcaccgcccg cgcgcgcgtc aggatcgtcc cctcacgggt cttcaggacg
     3601 aggctgtccg ggagcgcgtc ccgctcgtcc ttcggaagag cggtccgaaa ggcgcggctc
     3661 tgcagcggcg cgaatcggaa gcgcgcgaag cgatcctcgg cgaggaggaa acgcaccgcg
     3721 ccgtggcaga ggccgcaggt cccgtcgtag aacagcgtct cgggatgttt gtccgccccc
     3781 ctggccttcg cgcgccggcc cgccggggcg atccatcccg gatcgaacga caggaggccg
     3841 agcagtccga ccgccacgac gtccgccgtc cccgcgccgc gccccagaac gcgcagcacc
     3901 aggagcacga atgccgcccg gtgcacccag ggcatccacg ccggcatgtc ccaatcgccg
     3961 cggggatcga cgcggccccg cgccgccatc gatccgtacg gagcggccgg gaccaacagg
     4021 tgcgccatga ggaaccacgg ccagacgagg acgcgggcat cgagcggcgc gggcgcgacg
     4081 ccggccaggg cgggtgtgac ggtgacgagg cccaggacga tgagaacgag gatcgccgcc
     4141 gcgcgatcgc gccaccctac ggcgaggagc agggtccccg cgaccgccgc cgcgagcagg
     4201 atccggattc cccacgtcgc cccgggaagg agccgcagca ggtgcgcggc gaggaggatg
     4261 ccgaggagga cgcggcagag gctgtactga ccgccggacc agccggagcg caggcgcacg
     4321 cgggtcgaga ttagcacgcc cttcgcgagc ggcgtggtac gattccggcc cgcgatccgg
     4381 cccgcatccg cggcttcgaa ggagactccg cccgcatgaa tcccgtgaag cgtcccgcgt
     4441 tcgcccccgt gcccgtcgag gggccatgag cggcgggccc gacgctcccc ggcgcggccg
     4501 cggggccctg agcaaccgcg aggggcgcta cgagcgcacc cgcgtcgaga ccggaccgga
     4561 catcgagccg gatcccgacg tcgatccgga aggggagccg ccgccgctcg acaccgtcgt
     4621 cacgccggag cgcacccgct ccatcctgag caccaacgac tcgcccgacg tcgggttcga
     4681 ccagtccatc aatccctacc gcggttgcga gcacggctgc
//
LOCUS       DASRIQ010000343         8250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2210859, whole genome shotgun sequence.
ACCESSION   DASRIQ010000343 DASRIQ010000000
VERSION     DASRIQ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210859"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="VFD06_08175"
     CDS             complement(<1..1709)
                     /locus_tag="VFD06_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN03553.1"
                     /translation="MRRVLAYLLAVIATGAAVYGRVLLNPLLEERLPFITLFGAVALA
                     VWVGGFGPAVVAATLGYVAVARLVFEPGSLLSLDHPAGLVGVVTYAFTCLLIVALGSG
                     LRRSRGRAEASARQVRDQKLALEYEIAEHHRTEQALRHSEEDLERLAERIPIALTRCS
                     RDHRFVFVNRAAGDLFGRPAADLAGRPIDEVYDPEGLAVIAPYVERALRGEDVEFEAE
                     FSDARAGRRFVHVIYTPDRDDRGGITGWFGSIQDITGRKAAEAALQERERLFRTLVVS
                     QGQSTWQYRPGGTPITQISEEATAWWRAFTGQTEQERTARDGTGWLDAVHPDDRETAF
                     ANWREIREAKGATTASYRVRRKVDGAWRWLAVRGVPVIGPGGVIAEMAGTIADVTEQK
                     EAERTRSESESRFRLMADTAPVLVRLSGADAKSTWFNKPWLAFTGRSMEQEIDDGWTE
                     NIHPDDRERCLRTYAEEFGARRPFSMEYRLRRHDGSWRWLIDQGVPLFGGDGTFTGGI
                     GSCFDITDRKGTEDALREGDRRKDEFLATLAHELRNPLAPVRNAVEVLRLKGSPPPRC
                     SGRA"
     gene            complement(1711..3411)
                     /locus_tag="VFD06_08180"
     CDS             complement(1711..3411)
                     /locus_tag="VFD06_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN03554.1"
                     /translation="MPRRTDPPVPTRRHRPGTSTSRLRRQLRERTVALKDLLEDARSR
                     YVSLFDMAPVGYAVMDENGRIDDLNLTLCAMLGQLRAYMVGLPFLLCVIPEDRPRLLK
                     YLQDCRRSQGQSIPCDLSLLGSTRQGTLPVHLSTRRIAGVNGHGAYLMAILDVRERAE
                     ALRALEATGHQLRRSAEVLEERTSEAERATGRLRDLAREVNRAEQRERQRIARLLHDD
                     LQQLLVSAKMRLGLLQPGERRPAQFSEVATLLDAAIDSSRALTSQLSPPLLQDAGLEA
                     GLRWLARSFQEKHGLRVDLHLGLGSTPMDLSDRDFLFHAAREILFNVVKHARIRKADL
                     TAKVRNHHVSLEVADGGVGFDAAILDEESAADRLGLRTLRERAQLLGGGLVLETKPGN
                     GARVRVSFPLSDVAAPAKEPVAAEAAPHPAPDIPASGLVRVLIADDHRVVREGLQSFL
                     SRERWLEIVGQAADGRQAVLMTRELQPDVVIMDISMPELNGIEATRIITGENPHVRII
                     GLSMHEQEDMAAAMLGAGAERYLTKGGPAEDLVKAVRDLSVPPDPGRESGEPRPDSPA
                     "
     gene            complement(3395..6412)
                     /locus_tag="VFD06_08185"
     CDS             complement(3395..6412)
                     /locus_tag="VFD06_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HZN03555.1"
                     /translation="MPPRTRKKGMKSGPPPARRSAGKAPRPPRSPRAGRSDLDLPIVV
                     GVGASAGGLEAFTQLLEALPNDTGMAFVLVQHLAPRHESALPTLLGPVSGMGVEQARE
                     GLRPLPNHVYIIPPNSQLTIARGKLHLSPRPAGPGQHTPIDHFFRSLAQECGSRAIGV
                     ILSGTASDGSMGLREIKAAGGIAIAQDPNTAAYGGMPHAAVATGVVDLVLPVPAIAEA
                     LVQFRDHPYVAPPSASPETQVSGELRASEAQLQRIFTMLRNAKGVDFSLYKPPTIKRR
                     IQRRMALHKLEAVDAYIAHLRDSSAEVDALYHDILIQVTHFFREPEALNGVARAVFPA
                     LLKDRPPDLPLRIWVPGCATGEEVYSVAILLLEALDSASWSLPIQIFGTDVSDGAVEY
                     ARNGVYPEAIREHVSPERLRKFFTHVDGTWRISKKVRELCVFARQDLSRDPPFSRLDL
                     IVCRNVLIYLGAPLQKRLMNVFHYALKSSGFLVLGKAETVGLSNDLFTPLDKKRRIYW
                     RRPLERLQGTLFASDVQKQRLVTQGRRGPAPEPGTQSIVGQANQHLLEHYTPPAVIVD
                     ANLHIVQFRGQTGAYLEPAPGNASLNLLKMAREGLLYGLRTALHAARKRGQRVRKDGL
                     RVRSNGGYRTLGIEVSPIGAAGRDLHYLIVFHEASAPAEAVSPMPPPGRSRREARDEQ
                     RVARLQQELAASRDYLQSMIQDLEAANEELQSANEEILSSNEELQSTNEELDTAKEEL
                     QSINEELNTVNEELHGRNEELSRVNSDLLNLLGTVQIAIVIVNSDLQVRRFTPMAERL
                     FNLIPGDLGRPIGNIHPTFEGPDLPALITEVIDTMSGRTCEVRDRDGRWFSLTVRPYK
                     NVANAIDGAVMVLHDIDEARREGARAAYNRDLARGAMDLAPDPLLVVDADLKVTSVNR
                     AYCRRFQVTEEETVGRPVADLPGGPWKNAAVGAALGRLLERAEAGAETTVSANGRERP
                     DAKIVARRLAAEDGRGAILMVLSEGTRRDAPAH"
     gene            complement(6754..7101)
                     /locus_tag="VFD06_08190"
     CDS             complement(6754..7101)
                     /locus_tag="VFD06_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03556.1"
                     /translation="MRGSLGIRLLALALLGWSGILLRQVAAGHVLGRNRPLTFAAAAL
                     LALALLSAVALWGRSSHAFSLYALWAIAAMATFVLLRLHLSGSAHLVRFMPTILTTGL
                     VFAAGAVLLRRAS"
     gene            complement(7106..7801)
                     /locus_tag="VFD06_08195"
     CDS             complement(7106..7801)
                     /locus_tag="VFD06_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZN03557.1"
                     /translation="MRSRHVERHRTGRVGWLRAAVLGANDGIVSTTSLVVGVAASGAG
                     RHAVLVAGAAGLVAGAMSMAAGEYVSVSSQADTERAELDREKSELASSPEAEETELAG
                     IYADRGLEPVLARQVAQGLMAHDALGAHARDELGITETLTARPLQAALASAASFAVGA
                     ALPLLAAGLAPEERLALVASGSSLACLGLLGALAARAGGAPLLAGAGRVLFWGILAMT
                     ATAGVGSLFHAAP"
     gene            7840..>8250
                     /locus_tag="VFD06_08200"
     CDS             7840..>8250
                     /locus_tag="VFD06_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03558.1"
                     /translation="MRRVVRGLLLALVAIELLYVVPVNFVLNTPLVSRLVNRRPERFR
                     IEWSWALSPWPGFVVLRGVETGGQSRTIQWEAGLRKVSATFALRPLRRRVVELHEVRA
                     DGVRYTQRPRLPAGQEPPPDAADWPPIARKNPPDP"
BASE COUNT         1149 a         2926 c         2970 g         1205 t
ORIGIN      
        1 gcgcgcccac tgcacctcgg gggtggggag cccttgaggc gcagcacctc gacggcgttg
       61 cgcaccggcg cgaggggatt gcgcagctcg tgggccagcg tcgccaggaa ctcgtccttg
      121 cgccggtccc cttcgcgcag agcgtcctcg gtccccttgc ggtcggtgat gtcgaagcac
      181 gagccgatcc ccccggtgaa ggtcccgtcg ccgccgaaga gcggcacgcc ctggtcgatc
      241 agccagcgcc aggacccgtc gtggcgtcgc agccggtatt ccatggagaa cgggcggcgg
      301 gccccgaact cctcggcata ggtccgaagg cagcgctcgc gatcgtcggg gtggatgttc
      361 tcggtccagc cgtcgtcgat ctcctgttcc atgctccggc cggtgaacgc gagccacggc
      421 ttgttgaacc aggtgctctt cgcgtcggcg ccgctgagcc ggacgaggac cggcgccgtg
      481 tccgccatca gccggaagcg cgactcgctt tcgctgcggg tccgctccgc ctccttctgt
      541 tcggtgacgt cggcgatggt gccggccatc tcggcgatga cgccgccggg cccgatcacc
      601 ggcacgcccc gcaccgccag ccaccgccac gccccgtcga ccttccgccg gacccggtac
      661 gaggcggtcg tcgccccctt cgcctcccgg atctcccgcc agttcgcgaa cgccgtctcg
      721 cggtcgtccg ggtgcaccgc gtcgagccag ccggtcccgt cgcgcgcggt gcgctcctgt
      781 tccgtctggc cggtgaaggc gcgccaccag gcggtcgcct cctcgctgat ctgggtgatc
      841 ggcgtgccgc cgggccggta ctgccaggtg gactggccct gggagacgac cagcgtccgg
      901 aagagccgct cccgctcctg gagcgccgcc tcggccgcct tgcggccggt gatgtcctgg
      961 atcgagccga accagccggt gatcccgccc cgatcgtcgc ggtccggcgt gtagatcacg
     1021 tgcacgaagc gccggccggc gcgcgcgtcg gagaactccg cctcgaactc cacgtcctcc
     1081 ccgcgcaggg cgcgctcgac gtacggggcg atcaccgcca gcccctccgg gtcgtacacc
     1141 tcgtcgatcg gtcgtcctgc gagatccgcg gccggccgcc cgaacagatc tccggcggcc
     1201 cggttgacga aaacgaagcg gtgatcgcgg ctgcaccggg tcagggcgat cgggatcctc
     1261 tccgccagcc gctcgaggtc ctcctcgctg tgtcgcaacg cctgctcggt gcggtggtgc
     1321 tccgcgatct cgtattcgag ggccagcttc tgatcgcgga cctgccgcgc ggacgcctcg
     1381 gcgcgcccgc gggagcggcg cagcccgctc ccgagcgcca cgatgagcag gcacgtgaac
     1441 gcgtacgtca cgacaccgac caggccggcg gggtgatcga gggaaaggag agacccgggc
     1501 tcgaacacga gccgcgcgac ggcgacgtaa cccagggtcg ccgcgacgac cgccggtccg
     1561 aaacctccca cccacaccgc cagcgccacc gcgccgaaca gcgtgatgaa cggcaggcgc
     1621 tcttcgagga gaggattgag gaggacgcgc ccgtacaccg ccgcgcccgt cgcgatcacc
     1681 gccaggagat aagcgaggac gcggcgcatg tcaggcgggg gagtcgggac ggggctcgcc
     1741 gctctcccgc cccggatccg gagggacact caggtcgcgg accgccttca cgagatcctc
     1801 ggcggggccc cccttggtca gataacgctc ggcgccggcc ccgagcatgg ccgccgccat
     1861 gtcctcctgc tcgtgcatcg acagcccgat gatccgcacg tgcggattct cgccggtgat
     1921 gatacgggtc gcctcgatgc cgttcagctc cggcatcgag atgtccatga tcaccacgtc
     1981 cggctgcagc tcgcgcgtca tcaggaccgc ctgccgcccg tcggccgcct gtccgacgat
     2041 ctccagccag cgctcccggc tcaggaaact ctgcagcccc tcccggacga cccgatgatc
     2101 gtcggcgatg aggacccgca ccagccccga cgcagggatg tcgggcgccg ggtggggcgc
     2161 cgcctcggcc gcgaccggct ccttcgcggg cgccgcgacg tcgctgagcg ggaaagagac
     2221 ccgcacgcga gccccgtttc cgggcttggt ttcgaggacc aggccccctc ccaggagctg
     2281 ggcccgctcc cgaagtgtgc gcagcccgag ccggtcggcg gcgctttcct cgtccaggat
     2341 cgccgcgtcg aatcccaccc cgccatccgc cacctcgagg ctgacgtggt ggttccgcac
     2401 cttcgccgtg aggtcggcct tgcggatccg ggcatgcttg acgacgttga acaggatctc
     2461 ccgggccgcg tggaacagga agtcccgatc gctgaggtcc atcggcgtgg agcccagccc
     2521 gaggtgcagg tcgacccgca gcccgtgctt ctcctggaac gagcgggcga gccagcggag
     2581 ccccgcctcg agtccggcat cctgcagcag gggggggctg agctgcgacg tcagggctcg
     2641 ggaggagtcg atcgccgcgt ccaggagcgt cgccacttcc gagaactgcg ccggtcgccg
     2701 ctctcccggt tgcaggagac cgaggcgcat cttggccgac accagcagct gctgcaggtc
     2761 gtcgtgcagg agccgcgcga tgcgctggcg ctccctctgc tcggcccggt tcacctcccg
     2821 cgcgaggtcg cgcagcctgc cggtggcgcg ctccgcttcg ctcgtccgct cctcgagcac
     2881 ctcggccgat cggcgcagct gatggccggt cgcctcgagc gcgcggaggg cctcggcccg
     2941 ctcgcgcacg tcgaggatcg ccatcaggta ggcgccgtgg ccgttgacgc cggcgatgcg
     3001 gcgcgtggac aggtggaccg gcagggtccc ctggcgcgtg ctcccgagga gggacaggtc
     3061 gcaagggatc gactgcccct gcgagcgccg gcagtcctgc aggtacttga ggagccgggg
     3121 gcggtcctcc gggatgacgc agaggaggaa aggcagcccg accatgtagg cgcggagctg
     3181 gccgagcatg gcgcacagcg tgagattcag gtcatcgatg cggccgttct cgtccatcac
     3241 cgcgtagccg accggcgcca tgtcgaagag cgacacgtag cggctgcggg catcctcgag
     3301 caggtctttg agcgccacgg tccgctcgcg cagctgccgc cgcagccggg acgtactggt
     3361 cccagggcgg tgccggcgag tcgggacggg aggatcagtg cgccggggca tcccgtctcg
     3421 tcccttcgga gaggaccatc aggatggcgc cgcgaccgtc ctcggcggcc aggcgccggg
     3481 cgacgatctt cgcgtccggc cgctcgcgcc cgttcgcgct cacggtggtc tcggcccccg
     3541 cctccgcccg ctccagcagg cgcccgagcg cggcgccgac cgccgcgttc ttccacggtc
     3601 cgccggggag atcggccacc ggccgtccca ccgtctcctc ctcggtgacc tggaagcggc
     3661 ggcagtaggc gcggttgacc gacgtcacct tgagatcggc gtcgacgacg agcagcggat
     3721 cgggggccaa atccatcgcc ccgcgcgcca gatcgcggtt gtacgcggcg cgcgccccct
     3781 cgcggcgggc ttcgtcgatg tcgtgcagca ccatcacggc gccgtcgatg gcgttggcga
     3841 cgttcttgta cgggcgcacc gtcaggctga accagcgccc gtcgcgatcg cgcacctcgc
     3901 aggtgcggcc gctcatcgtg tcgatcacct cggtgatcag ggctggaagg tcggggcctt
     3961 cgaaggtggg gtggatgttg ccgatggggc gtccgagatc cccggggatc aggttgaaca
     4021 gcctttccgc catcggcgtg aagcggcgga cctggaggtc cgagttgacg atgacgatgg
     4081 cgatctgcac ggtgccgagg aggttcagga gatcgctgtt gacgcggctg agctcctcgt
     4141 tgcggccgtg cagctcctcg ttgaccgtgt tgagctcctc attgatcgac tgcagctcct
     4201 ccttggcggt gtcgagctcc tcgttggtgc tctgcagctc ctcgttgctc gacaggatct
     4261 cttcgttggc cgactgcagc tcttcgttcg ccgcctcgag gtcctggatc atcgactgga
     4321 ggtagtcgcg gctggccgcc aattcttgct ggaggcgcgc cacgcgctgt tcgtcgcgcg
     4381 cttcgcgccg ggagcgacct ggtggcggca tgggggacac cgcctccgcc ggcgccgacg
     4441 cctcgtggaa gacgatcagg tagtgcaggt cccgcccggc ggcgccgatg ggggagacct
     4501 cgatgccgag ggtgcggtag ccgccgttcg accgcaccct gagaccgtcc ttgcgcaccc
     4561 gctgccccct cttgcgggcg gcgtggagcg ccgtccggag gccgtacagg agcccctcgc
     4621 gcgccatctt gaggagattg aggctggcgt tgccgggtgc cggctcgagg taggcgccgg
     4681 tctggccgcg gaactggacg atgtgcagat tcgcgtcgac gatcaccgcg ggcggggtgt
     4741 aatgctccag gaggtgctgg ttcgcctggc cgacgatgct ctgcgtcccc ggctcggggg
     4801 ccggcccgcg ccgcccctgg gtgacgagcc gctgcttctg cacgtcggag gcgaacaggg
     4861 tgccttgcag ccgttccagc ggccggcgcc agtagatccg ccgcttcttg tcgagcggcg
     4921 tgaagaggtc gttgctcagc ccgaccgtct cggccttgcc gaggacgagg aatccggagg
     4981 acttgagcgc gtagtggaac acgttcatca gccgcttctg cagcggcgcg ccgaggtaga
     5041 tgagcacgtt gcggcagacg atcaggtcga gccgcgagaa gggggggtcg cgcgacaggt
     5101 cctgccgggc gaagacgcac agctcgcgca ccttcttcga gatgcgccag gtcccgtcga
     5161 cgtgtgtgaa gaacttgcgc aggcgctcgg gggagacgtg ctcgcggatc gcctcgggat
     5221 agacgccgtt gcgggcatac tccaccgccc catccgagac gtcggtcccg aagatctgga
     5281 tcggcaggct ccacgacgcg ctgtcgagcg cttcgagcag caggatggcg acggagtaga
     5341 cctcctcgcc ggtggcgcag cccgggaccc agatgcgcag ggggagatcg ggaggacgat
     5401 ccttgaggag cgccgggaag accgcccggg cgaccccgtt gagcgcctcc ggctcgcgga
     5461 agaaatgcgt cacctggatg aggatgtcgt ggtacagcgc gtccacctcg gcggaactgt
     5521 ccctcaggtg ggcgatgtac gcgtcgaccg cctcgagctt gtgcagcgcc atgcgccgct
     5581 ggatgcggcg cttgatggtg gggggcttgt agagggagaa atcgaccccc ttggcgttgc
     5641 gcagcatggt gaagatcctc tgcagctgtg cctcgctcgc gcgcagctcg ccggacacct
     5701 gcgtctcggg ggacgcggac ggcggggcga cgtaggggtg atcgcggaac tgcaccaggg
     5761 cctcggcgat cgccgggacc ggcagcacca gatcgacgac gccggtggcc acggcggcgt
     5821 gcggcatccc gccgtacgcc gcggtgttcg gatcctgggc gatggcgatc ccgccggccg
     5881 ccttgatctc ccggagcccc atcgacccgt ccgacgccgt ccccgagagg atgacgccga
     5941 tggcgcggct gccgcactcc tgcgccagcg agcggaagaa atggtcgatc ggcgtgtgct
     6001 gtccggggcc ggcggggcgg ggggagaggt gcagctttcc ccgggcgatc gtcagctgcg
     6061 agttcggcgg gatgatgtag acgtggttgg gcagcgggcg cagcccttcg cgggcctgct
     6121 ccacgcccat gccggacacc ggcccgagca gggtgggcag ggcgctctcg tggcgcggcg
     6181 ccaggtgctg caccaggacg aacgccatgc cggtgtcgtt cggcagcgct tcgaggagct
     6241 gggtgaacgc ctcgaggccg ccggcggagg cccccacccc gacgacgatc ggaagatcca
     6301 ggtcggagcg tccggcccgg ggtgagcgcg gcggcctggg ggccttcccg gcgctcctgc
     6361 gggccggggg ggggccgctc ttcattccct tcttccgggt gcgcggcggc atccacgacc
     6421 tccgttcagt ggtgacgctc gatcaggatg cggcgcagga tgagggggcg ccgttcgagc
     6481 gcccccgcgg atcccccgat cacgacgatg tcgtgcaagt cgagcctcca cgggccctgc
     6541 ggcgaaacgg gttgctctgc gaagattcgt tcgatcctat caccactcct ccggggtccc
     6601 ggcgcggagc gcgcagacga gtcatggcgc cgggcgatgc ggcgcgggcc gacctggtcg
     6661 cccgaacgac ccggttcatc ggattggtcc acttggcaat gtatcgaagg aaacggcggt
     6721 cgcccacacg tggaaatgcg gatccgtggt ttttcagctc gcccggcgga ggaggaccgc
     6781 cccggccgcg aacaccagtc cggtcgtcag gatggtcggc atgaagcgca ccagatgggc
     6841 cgaacccgac agatgcaggc gcaggagcac gaaggtcgcc atcgccgcga tcgcccagag
     6901 cgcgtagagg gagaaggcat ggctcgagcg tccccagagg gccacggcgg acagaagcgc
     6961 cagcgccagg agcgcggccg cggcgaaggt gagcggccgg ttgcgcccca ggacgtgccc
     7021 ggccgcgacc tgtcgcagga ggattccgct ccatccgaga agcgccaggg ccaggaggcg
     7081 gataccgagg gatccccgca tgcggctacg gagcggcgtg gaacaggctc ccgacccccg
     7141 cggtcgccgt catcgccagg atcccccaga acagcacgcg cccggcgccg gcgagaagcg
     7201 gcgcccctcc ggcccgtgcc gcgagcgcgc cgaggagtcc caggcaggcg agcgacgagc
     7261 cggaggcgac gagcgccagg cgctcctccg gcgcgaggcc ggccgcgagg agcggcaacg
     7321 cggcgccgac ggcgaaggag gcggcggagg cgagcgccgc ctggagcggc cgcgccgtga
     7381 gagtctcggt gattcccagc tcgtcgcgcg cgtgcgcccc cagagcgtcg tgcgccatca
     7441 gcccctgcgc cacctgccgt gccagcaccg gctcgagacc gcggtccgcg tagatgccgg
     7501 cgagctccgt ctcctccgcc tcgggactcg acgccagctc cgacttctcg cgatccagct
     7561 ccgcccgctc ggtgtcggcc tgcgagctca ccgagacata ttcgccggcc gccatcgaca
     7621 tggcgccggc gacgagcccc gccgccccgg ccacgagcac cgcgtgccgc ccggcccccg
     7681 aagccgcgac gccgacgacg aggctcgtcg tcgagacgat gccgtcgttg gcgccgagca
     7741 cggcagcgcg cagccagccg acgcgcccgg tcctgtgtcg ttccacgtgt cgagaccgca
     7801 tgcggccggc gaggttagca tgcgccgatg gcgattcgca tgcgcagagt cgttcggggg
     7861 ctgctgctcg ccctcgtcgc gatcgagctg ctctacgtcg tcccggtcaa cttcgtcctc
     7921 aacacgcctt tggtctctcg cctcgtcaac cggcggccgg agcgcttccg gatcgagtgg
     7981 tcgtgggcgc tgagcccgtg gcccggcttc gtcgtcctgc gcggcgtcga aaccggcggg
     8041 cagtcgcgca ccatccagtg ggaggcgggg ctccgcaagg tcagcgccac cttcgcgctg
     8101 cggccgctcc ggaggcgcgt cgtcgagctg cacgaggtcc gggcggacgg cgtgcgctac
     8161 acgcagcggc cgcgcctccc ggcgggccag gagccgccgc cggacgcggc ggactggccg
     8221 ccgatcgccc ggaagaatcc ccccgaccca
//
LOCUS       DASRIQ010000344         6906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1193760, whole genome shotgun sequence.
ACCESSION   DASRIQ010000344 DASRIQ010000000
VERSION     DASRIQ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193760"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFD06_08205"
     CDS             <1..555
                     /locus_tag="VFD06_08205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN03559.1"
                     /translation="WVLRLAIVPYDLTHLPLSRPDRLPLHLMPLLTAMFVHAGWLHLL
                     GNLLYLWIFGNNVEDVMGHGRFLAFYLLCGLLAAAVQIGSQPESRIPMIGASGAIAGV
                     LGAYLVQFPTARVRTLLFLVVIVRVVALPALIVLGVWLLLQVIAAGRGGGQGVAWFAH
                     LGGFLAGALLIVPFRRRRPRQALF"
     gene            complement(552..1136)
                     /locus_tag="VFD06_08210"
     CDS             complement(552..1136)
                     /locus_tag="VFD06_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN03560.1"
                     /translation="MIPPIARTAPSIVGLTGPNASGKGEVAKFLAARGFTVHSLSDIV
                     REAAARAGLEPSRDNLIATGVALRAAEGPGVLAARILPRLAEEAVVDSIRGPAEVAVL
                     RTLPTFVLVGVDAPMPLRFERSLLRGRLGDGASLQEFERQEERENASAETGQQLRRTL
                     ALADRVLVNDGTIADLHARALVLFREIGLVLPSS"
     gene            complement(1133..2014)
                     /locus_tag="VFD06_08215"
     CDS             complement(1133..2014)
                     /locus_tag="VFD06_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03561.1"
                     /translation="MAHRSNTLRASTLFLVAAALVASGAAARADQIVYFVNGKAITVK
                     KVEKGDRLTILEIEGGGRIGIPTVQIDRIEELAVAAAVPQVAVPAVLPAPQPEPQAAN
                     QIPPPPAAASTLPAGPGTGGRVAANSPANGAKPLAFGGDPSEEAAPVRPRPGFQSTPP
                     GNGGSTTAMTPGQQNQRNSNMMTGGPGAFQRGRPGGRNGKNGRLPAAAYLPPKNDPGS
                     AAGAPAGQPGAAQQPPPGTSPVAPPPTAGAPPPPPPPPDPAEADPGSSDGSADDGAAP
                     KTGTEEPASGDGDSGQN"
     gene            complement(2137..3162)
                     /locus_tag="VFD06_08220"
     CDS             complement(2137..3162)
                     /locus_tag="VFD06_08220"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HZN03562.1"
                     /translation="MSRRVEARAPTRIDLAGGTVDLWPLSLLTRDPLTVNAAIDLHAT
                     ATVERATGAGLAIASRDRGVSGHVPAGADLHAALAAVPKDLEFIARLAHHFLGREADG
                     VRIVTDCAAPAGSGLGGSSTLGIALAAALDRFTARGLDPERLLGLVRGIETQVLAIPT
                     GEQDYHPAQRGGVLALRYTVEGTVVEPLSADLEALKRRTVLVFTGISRNSGLSNWDIC
                     KRYLDGDRSVREALQATSDAAHAVRKAILASDYDAVGPALAEEWRARLRLSPAVTDAR
                     IDALIASGIEAGARAGKVCGAGGGGCLVLWTPDDRREAVRERLRQAGGTILDFSYVSE
                     GVRLTVS"
     gene            3226..5508
                     /locus_tag="VFD06_08225"
     CDS             3226..5508
                     /locus_tag="VFD06_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03563.1"
                     /translation="MTVRTASTPHSERSFWSGAARLVLAAVLLLLPLAHSAGLSDPFD
                     LPKEIILCGGALLLLACLVFGSARIGAGASLVGGLPVAAATLVALGAAGLAVARSASA
                     GIAASAFLTLAALVVVAASVPLAVRRPRDARLLMVAAIGSLSLAALASLAQIFRPGFN
                     LAIGDISIVPPAPAGGTFGDPGLLAQAILVALPLGAGAAALLSGAWRLALGGTLGILA
                     AALLYGGRPEGWLGACGVVAVMLGTRVARALRQADPWSELVPDPGGPTARTILAAGAI
                     VALALAAARLPGMGPGGAPPAPLTQVGLLAPTTGDVAADRAAGTTGSLALLGHHPLGT
                     GPGTWRHAVLEVAWTAVPNSPFSLSHQAVHAGQSFLEAAAELGLAGAAAFLFLLGVIL
                     ARAARTFLRDRGEWGTIAATGFATILATGLVACLGSPFQEVAPAALVAFACGLAVAAH
                     RQSFGIASPDAAALIASARARVVTLGAGIAALLVFGAGIALVLPARVAAARLTLAAQA
                     RLGAGDARQAVQLLSLPAARRAADHLPHALRGQAALRLGAYQEASDAFGDTLKRSPSF
                     VSAYLGRAAAEEMLGHYDRAASDLDAALALWPGSYDILMARGRLDARRGRLEQAIAAY
                     QEAGKAGPKAGDPWFALGEILLRRGESDRAIEAFHLCLQKNPRFPRINLALASAYEMR
                     GMNDMAIGHLQREAAIDDRAVEPRLRMANLFHAEGHDCDARDALVAARDLETDADRRT
                     RILELVDRMEASCQQQQRRR"
     gene            complement(5527..6441)
                     /locus_tag="VFD06_08230"
     CDS             complement(5527..6441)
                     /locus_tag="VFD06_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03564.1"
                     /translation="MPRSGSLVAGIFALLLLPSAALAAPAPAPSAAASDETLFREIRI
                     SLYDSDWPAVLRLSESLLKRYPASPLAPQAEFHRARALARLPGREGEGIGAFRSFIAE
                     HPAERDRLLVDQAWSMLFSLACQGKRRISPGCAAMLSEGIGDRSLYVSTLAAIRAADA
                     GDPHVRRAAVPILKRAYDLENDTEIRNEILIALLKIDPREVPPPPPAPPAPAAPPAGV
                     PPLPSLVKMTIYNKAAKRYDLRINVPVAFAQILVNALGTEERSALKDSAAKKGVDLDD
                     IFRAIEEKGQGRLVDVDAPDTRIEVWVE"
     gene            complement(6434..>6906)
                     /locus_tag="VFD06_08235"
     CDS             complement(6434..>6906)
                     /locus_tag="VFD06_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03565.1"
                     /translation="APSRQATVLPTAPAEAGAGAEPAVATEAARYLEAGVARRAAAAS
                     LRDGRALLLDLLEAPVRSRREDGRYDVALERARSKELLRRLAVQRRALEEPGDRRLAE
                     MLGALSDLLLDVAEMDDYADGPQLRALREAIERRQVLLRLDLMTRETEEGEARA"
BASE COUNT          792 a         2570 c         2538 g         1006 t
ORIGIN      
        1 tgggtgctgc ggctggcgat cgtcccctac gacctgacgc acctgccgct ctcccgtccg
       61 gatcggctgc cgctgcacct gatgccgctt ctcaccgcga tgttcgtgca cgccggctgg
      121 ctgcacctcc tgggaaacct cctgtacctc tggatcttcg gcaacaacgt cgaggatgtg
      181 atggggcacg ggcgattcct cgcgttttat ctgctctgcg gacttcttgc ggcagcggtg
      241 cagatcggct cgcagccgga gtcgcgcatc ccgatgatcg gcgccagcgg ggccatcgcc
      301 ggggtcctgg gcgcctacct cgtgcagttc cccacggcgc gcgtccgcac gctgctcttc
      361 ctcgtcgtga tcgtccgggt ggtggcgctg ccggcgctca tcgtgctcgg cgtctggctc
      421 ctcctccagg tcatcgccgc gggccgcggc ggcgggcagg gggtggcctg gttcgcgcac
      481 ctgggagggt tcctggccgg cgccctgctc atcgtcccgt tccgccgcag gcggccccgc
      541 caggcgcttt tctaggacga aggcaggacg agcccgatct cccggaagag gacgagggcg
      601 cgggcgtgca ggtcggcgat ggtgccgtcg ttcacgagga cccgatcggc cagcgccagg
      661 gtgcgccgca gctgctgccc ggtctcggcg ctggcgttct cccgctcctc ctgccgctcg
      721 aattcctgga gcgacgcgcc atcccccagt cggccgcgca gcagggatcg ctcgaagcgg
      781 agcggcatcg gagcgtcgac gcccacgagg acgaacgtcg ggagcgtccg gagcaccgcg
      841 acctcggcgg ggccgcggat cgaatcgacc accgcctctt ccgcgaggcg cggcaggatc
      901 cgggccgcca ggacgcccgg cccctcggcg gcgcgcagcg ccaccccggt ggcgatgagg
      961 ttgtcgcgcg acggctcgag ccccgcacgg gcggccgcct cgcgcacgat atcggagagc
     1021 gagtggacgg taaacccgcg ggcggcgaga aacttcgcca cctccccctt cccggaggcg
     1081 ttcgggccgg tcagcccgac gatcgacggt gcggtgcggg cgatgggcgg gatcagttct
     1141 gcccggagtc gccgtcgccg gacgccggct cttcggtccc cgttttgggg gccgcgccgt
     1201 cgtccgcgga gccgtcggag ctgcccggat ccgcctcagc cggatcgggc ggcggcggag
     1261 gcggtggcgg tgcgccggcc gtgggcggcg gcgccacagg cgacgtgccg ggcggcggtt
     1321 gctgggccgc gccgggctgg cctgccggcg ccccggccgc gctgccagga tcgttcttcg
     1381 gcggcagata ggcggcggcc ggcaggcggc cgttcttgcc gttcctgccg ccggggcgcc
     1441 cgcgctggaa ggcgcccggg cctcccgtca tcatgttgct gttccgctgg ttctgctgac
     1501 ccggcgtcat cgcggtggtg ctgccgccgt tgccgggcgg ggtggactgg aatcccggtc
     1561 tcggccgtac cggagccgcc tcttccgagg ggtctccgcc gaacgccagc ggcttcgccc
     1621 cgttcgccgg actgttcgca gcgacccgcc cgccggtccc cggaccggcc ggaagcgtcg
     1681 acgcggcggc gggaggaggc gggatctggt ttgcggcctg gggctccggc tgcggcgccg
     1741 gcaggaccgc cgggactgcc acttgaggta cggccgccgc caccgcgagc tcctcgatcc
     1801 ggtcgatctg cacggtcggg atgccgatcc ggcctccccc ttcgatctcg aggatcgtca
     1861 gccgatcccc tttctcgact ttcttgacgg tgatcgcttt gccgttcacg aagtagacga
     1921 tctggtcggc gcgcgcggcg gcgccggacg cgacgagcgc ggccgccacg aggaacaggg
     1981 tcgaggcgcg gagcgtgttc gatcggtgag ccatggacat gagcaccctc agggaaaagg
     2041 tctcccggtt tttcgagtca gccggaaggg gggcgtcggg agtgtaccgc gagagtcctg
     2101 ggggagcaat cgaaatcccc cccagaccgt gccgcgttac gaaaccgtga gccggacccc
     2161 ctccgaaacg taggagaagt cgaggatcgt cccgcccgcc tgccgcagcc tctccctcac
     2221 ggcctcccga cggtcgtccg gggtccagag gacgaggcag cctccgcccc ccgcgccgca
     2281 gaccttcccc gcccgggccc ctgcctcgat cccgctggcg atgagcgcgt cgatccgcgc
     2341 gtcggtgacg gcgggggaca ggcgcaggcg cgcccgccac tcctccgcga gcgccgggcc
     2401 gaccgcgtcg tagtcggagg ccagaatcgc cttgcgcacg gcatgcgccg cgtcgctcgt
     2461 cgcctgcagc gcttcccgca ccgatcgatc gccgtcgagg taccgcttgc agatgtccca
     2521 gttcgagagc ccgctgttgc gcgagatgcc ggtgaagacg agcacggtcc gccgcttcag
     2581 cgcctcgaga tccgccgaca gcggctcgac gacggtcccc tccaccgtgt agcgcagcgc
     2641 caggaccccg ccccgctggg cagggtggta gtcctgctcg ccggtgggga tggcgaggac
     2701 ctgggtctcg atgccgcgca cgagccccag gagtcgctcc gggtcgagac cgcgcgcggt
     2761 gaagcggtcg agagccgccg cgagggcgat cccgagcgtc gacgatcccc ccagccccga
     2821 gccggccggt gcggcgcaat cggtgacgat gcgcacgccg tccgcctcgc ggccgaggaa
     2881 gtggtgcgcc agccgcgcga tgaactcgag atccttcggg actgcggcga gggccgcgtg
     2941 caggtcggcg ccggcaggga cgtggccgga gacgccgcga tcgcgcgagg cgatggcgag
     3001 gccggccccg gtggcgcgct cgaccgtcgc cgtggcgtgc agatcgatgg ccgcgttcac
     3061 ggtcagggga tcgcgcgtca ggagcgacag gggccacagg tcgaccgtgc cccccgccag
     3121 atcgatgcgc gtcggcgccc gcgcttccac tcgtcgcgac atggcggccg atcctacacc
     3181 ggtcctccgg aaggtccgcc gattctgtgg tatcgtcccc gccccgtgac cgtccgcacg
     3241 gcctccacgc cccattccga gcgttccttc tggtccggag cggcccggct cgtcctcgcc
     3301 gccgtgctcc tcctgctgcc gctggcgcat tctgcggggc tgtccgatcc gttcgatctt
     3361 cccaaagaga tcatcctgtg tggcggcgcg ctgctcctgc tcgcctgcct ggtgttcgga
     3421 tcggcgcgga tcggcgccgg cgcctcgctc gtgggcggac tcccggtcgc ggccgcgacc
     3481 ctcgtggcgc tgggcgcggc ggggctggcg gtggcgcgct ccgcctcggc cgggatcgcc
     3541 gcgtcggcgt tcctgaccct cgcggccctc gtcgtcgtcg cggcctcggt cccgctggcg
     3601 gtgcgacgcc cgcgggacgc ccgcctgctc atggtcgcgg cgatcggctc tctgtcgctc
     3661 gcggcgctcg ccagcctggc gcagatcttc cgtcccgggt tcaacctcgc gatcggcgac
     3721 atctcgatcg tccctcccgc accggcgggc gggacgttcg gcgatcccgg ccttctggcg
     3781 caggcgatcc tcgtcgcgct tccgctgggg gcgggggccg ccgcgctcct gtccggtgcg
     3841 tggcgtctgg cgctcggagg gacgctcggg atcctcgcgg cggcgctcct gtacggcggc
     3901 cggccggagg gatggctggg agcctgtggc gtcgtcgcgg tgatgctcgg cacccgcgtc
     3961 gcacgcgcgc tccggcaggc ggatccctgg tctgagctcg tgcccgaccc cggcggcccg
     4021 accgcgcgca cgatcctggc ggccggtgcc atcgtggcgc tggcgctcgc ggcggcccgg
     4081 cttcccggga tgggccccgg cggcgcgccg ccggcgccgc tgacgcaggt cggcctcctc
     4141 gctccgacga ccggcgacgt ggcggccgat cgggccgccg ggacgaccgg aagcctggcc
     4201 ctcctcggcc accacccgct cggcaccgga cccggcacct ggcgccacgc cgtcctcgag
     4261 gtcgcctgga ccgcggtccc caactccccc ttcagcctgt cgcaccaggc ggtgcacgcc
     4321 gggcagtcgt tcctcgaggc cgccgcggag ctcggcctcg ccggcgcggc ggcgttcctg
     4381 ttcctcctgg gggtcatcct ggcgcgggcg gcccggacgt tcctccggga ccgtggcgag
     4441 tggggcacca tcgccgccac cgggttcgcc acgatcctcg cgacggggct cgtcgcctgt
     4501 ctcggctccc cgttccagga ggtcgccccg gccgcgctcg tggcgttcgc ctgcggcctg
     4561 gcggtcgcgg cgcaccggca gtccttcggg atcgcctccc ccgatgccgc cgccttgatc
     4621 gcgtcggcgc gcgcacgggt cgtcaccctc ggcgccggta tcgcggccct cctggtgttc
     4681 ggcgcgggga tcgccctcgt gctgcccgcg cgggtggcgg ccgcgcgcct gacgctcgcg
     4741 gcgcaggcgc ggctcggagc cggtgacgcg cgccaggcgg tgcagctcct gtcgctgccg
     4801 gcggcccggc gcgccgccga ccacctgccg cacgccctgc gcgggcaggc cgcgctccgg
     4861 ctcggtgcgt accaggaggc ctccgatgcc ttcggcgaca ccctgaagcg ttccccctcc
     4921 ttcgtctcgg cgtacctcgg gcgtgcggcc gcggaggaga tgctgggcca ctacgatcgc
     4981 gccgcgtccg acctcgacgc ggcgctcgcc ctttggccgg ggagctacga catcctgatg
     5041 gcgcgcggac gcctcgacgc ccgccggggc cggctggagc aggcgatcgc cgcctaccag
     5101 gaggccggga aggcgggacc gaaggcgggg gatccctggt tcgcgctcgg cgaaatcctg
     5161 ctgcgccgcg gcgagagcga tcgggcgatc gaggcgttcc acctctgcct gcagaagaac
     5221 ccgcgcttcc cgcgcatcaa cctggccctc gcctccgcct acgagatgcg tggcatgaac
     5281 gacatggcga tcgggcacct gcagcgcgag gcggcgatcg acgaccgggc ggtcgagccg
     5341 aggctgcgga tggccaacct ctttcacgcc gaggggcacg actgcgacgc gcgcgacgcc
     5401 ctcgtggcgg cgcgcgatct ggagacggac gcggatcgcc ggacgcgaat cctcgaactc
     5461 gtcgatcgca tggaagccag ctgccagcag cagcagcggc ggcgttagga ccctgtccga
     5521 gaagcgctac tcgacccaga cctcgatgcg ggtgtcgggg gcgtccacgt ccaccagcct
     5581 cccctgcccc ttctcctcga tcgcgcggaa gatgtcgtcg aggtcgaccc ccttcttcgc
     5641 ggcgctgtcc ttgagggcgc tgcgctcctc cgtccccagc gcgttgacca ggatctgcgc
     5701 gaacgccacc ggcacgttga tgcgcaggtc gtagcgcttc gcggccttgt tgtagatcgt
     5761 catcttgacg agcgacggca gcggcggcac cccggccggc ggcgcagccg gggccggggg
     5821 cgcgggcggg ggcgggggca cctcgcgcgg atcgatcttc aacagggcga tgaggatctc
     5881 gttgcggatc tccgtgtcgt tctcgaggtc gtaggcgcgc ttgaggatcg gcaccgcggc
     5941 ccgcctcaca tgcggatccc ccgcgtcggc ggcgcggatc gccgcaagcg tcgagacgta
     6001 gagcgaccgg tcgccgatcc cctccgacag catcgcggcg cagccgggcg agattctccg
     6061 cttcccctgg caggcgagcg agaacagcat cgaccaggcc tgatcgacga gcaggcggtc
     6121 ccgctcggcc gggtgctcgg cgatgaagct ccggaaggca ccgatcccct ccccttcgcg
     6181 gccgggcaga cgggcgagcg cgcgggcgcg gtggaattcc gcctgcggcg ccagcgggct
     6241 cgccgggtac cgcttcagga gcgactcgct gaggcgcaac accgccggcc agtcgctgtc
     6301 gtagagggag atccggatct cgcggaacag cgtttcgtcg gatgccgccg ccgacggcgc
     6361 gggggcaggg gcggcgagcg ccgccgacgg aaggagcagg agcgcgaaga tccccgcgac
     6421 caggcttccg cttctaggca cgggcctctc cctcctccgt ctcgcgggtc atcagatcga
     6481 ggcgcagcag cacctggcgc cgctcgatgg cctcccgcag ggcgcgcagc tgcggcccgt
     6541 cggcgtagtc gtccatctcg gcgacgtcga ggagcagatc ggacagggcg ccgagcatct
     6601 cggccagccg ccgatcgccg ggctcctcga gggcgcggcg ctgcaccgcc agccgccgca
     6661 gcagctcctt cgagcgggcg cgctcgagcg ccacgtcgta ccggccgtct tcgcgccggg
     6721 agcgcaccgg cgcctcgagg agatcgagga ggagcgcccg gccgtcgcgg agcgaagccg
     6781 ccgccgcgcg gcgggcgacg cccgcttcga ggtagcgggc cgcctcggtg gcgacggcgg
     6841 gctccgcgcc cgcgccggct tcggccggtg ctgtgggaag aacggtcgcc tgccgggagg
     6901 gcgccg
//
LOCUS       DASRIQ010000345         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_389024, whole genome shotgun sequence.
ACCESSION   DASRIQ010000345 DASRIQ010000000
VERSION     DASRIQ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389024"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VFD06_08240"
     CDS             <1..1113
                     /locus_tag="VFD06_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN03566.1"
                     /translation="ALMTGFQDQIQAKILGVNAHLTILSGLSGRPIEGVDEALQRVAA
                     LPQVAAVAPVVFEKGMASSALNAAGTAVLVKGIDPEAEGRVTEWPRLVRGDVRILERT
                     DARGREPAFLGRDLAASLGVGPGDTVRVIIARPEVSPLLTVPRSREFLVAGVFESGFY
                     EYDTTRLYVGIGALRRFVGLPENGATAIEARLDDPRRIPERAEEVQRALGDAFYVNDL
                     VRMNRTFFAALRLEKLAMSVAIGLIIVVAALNIVSVLVLLVMEKVRDIGVLAALGATA
                     PDLRRIFALQGLIIAGCGTFGGALLGVVLAVTLDRTRMVRLPPDVYFIPYVPFHVRPL
                     DVLAVVAVTFGVSFLATLYPAWRAAQLDPSEALRYE"
     gene            1106..1813
                     /locus_tag="VFD06_08245"
     CDS             1106..1813
                     /locus_tag="VFD06_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN03567.1"
                     /translation="MSEAAPGAPLLRCRGLTRAFGSSAARVTILEALNLDVQAGEMVA
                     ILGESGAGKTTFLHLIGALDRPDAGRIEVRGREIGGATGAARAAYRNRDLGFVFQFHH
                     LLPEFSAVENAMMPLLIAGEPPAAARRRAADLLGELGLGAALDRRPAELSGGEQQRVA
                     IARAVVRRPLLLLADEPTGNLDERNAAAAFALLRDLHRSRGMTTLLVTHSERLATQCD
                     RILVMELGSLTPRSGDR"
     gene            1840..>2217
                     /locus_tag="VFD06_08250"
     CDS             1840..>2217
                     /locus_tag="VFD06_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZN03568.1"
                     /translation="MFEKYTEKAKKVLFLARYEASQMGSRVIGSEHLLLGLLKDGDEL
                     VQELFAKAAVNIELLRAEMEARGPSGEKQVTPIEIPFSEEAKKVLACAEEEAERLLHP
                     HVSDEHILLGLLRVEESAAGRLLA"
BASE COUNT          284 a          782 c          819 g          332 t
ORIGIN      
        1 gccctcatga ccggcttcca ggatcagatc caggccaaga tcctcggggt caacgcccac
       61 ctgacgatcc tctcgggcct gtccggacgg ccgatcgaag gtgtggacga ggcgctgcag
      121 agagttgccg ccctgcccca ggtggccgcc gtggcgcccg tggtgttcga gaaggggatg
      181 gcgtcgagcg ctctcaacgc cgccgggacc gccgtcctcg tcaaggggat cgatccggag
      241 gcggaagggc gcgtgacgga gtggccgcgg ctggtccgcg gcgacgtgcg catcctcgag
      301 cgcaccgacg cgcgcggccg cgagccggcc ttcctcggcc gcgacctggc cgcctcgctc
      361 ggcgtcgggc cgggcgacac cgtgcgggtg atcatcgcgc ggccggaggt gtccccgctg
      421 ctgaccgtcc cgcggagccg cgagttcctg gtcgccgggg tcttcgagtc cggcttctac
      481 gagtacgaca cgacgcgcct gtacgtcggg atcggggcgc tgcggcgttt cgtgggtctg
      541 ccggagaacg gcgccaccgc catcgaggcg cggctcgacg acccgcgccg catcccggag
      601 cgggccgagg aggtgcagcg tgcgctcggc gacgccttct acgtcaacga tctcgtccgc
      661 atgaaccgaa ccttcttcgc ggcgctgcgt ctcgagaagc tggcgatgag cgtggccatc
      721 ggcctcatca tcgtggtggc ggcgctcaac atcgtctcgg tgctggtgct cctggtgatg
      781 gagaaggtgc gggacatcgg cgtgctggcg gcgctcgggg cgaccgcccc cgacctgcgg
      841 cggatcttcg ccctgcaggg gctcatcatc gccggctgcg gcacgttcgg aggggcgctt
      901 ctgggcgtgg tgctggcggt gaccctcgac cggacccgga tggtccgcct gccgcccgac
      961 gtgtacttca tcccctacgt cccgttccac gtccggccgc tcgacgtcct ggccgtcgtg
     1021 gcggtcacct tcggggtctc ctttctcgcc acgctgtacc cggcatggcg cgcggcgcag
     1081 ctcgatccgt ccgaggcgct gcgctatgag tgaggcggcg cccggggccc cgctgctgcg
     1141 ctgccggggg ctgacgcgcg ccttcggctc ctccgcggcg cgggtgacga ttctcgaggc
     1201 cctcaacctc gacgtgcagg ccggcgagat ggtggcgatc ctcggggagt cgggggccgg
     1261 gaagaccacc ttcctgcacc tcatcggggc cctcgaccgt ccggacgccg gccgcatcga
     1321 ggtgcgcggc cgcgagatcg gcggcgccac gggcgcggcg cgcgccgctt accgcaaccg
     1381 cgatctcggt ttcgtcttcc agttccacca cctgctcccc gagttcagcg ccgtcgagaa
     1441 cgccatgatg ccgctcctga tcgcggggga gccgccggcg gcggcgcgcc ggcgcgccgc
     1501 ggacctgctg ggcgagctgg ggctgggggc cgccctcgat cggcgaccgg ccgagctgtc
     1561 ggggggcgag cagcagcgcg tggcgatcgc ccgggcggtg gtgcgccgcc cgctcctcct
     1621 gctcgcggac gagccgaccg ggaacctcga cgagcgcaac gcggccgccg ccttcgcgct
     1681 gctgcgggat ctccaccgca gccgggggat gaccactctc ctcgtcacgc acagcgagcg
     1741 gctcgcgacg cagtgcgatc gcatcctcgt catggagctc ggttccttga cgccccggag
     1801 tggcgaccgc tagaatcggg gagcggcgcc gggggcagca tgttcgagaa gtacaccgag
     1861 aaggccaaga aggtcctgtt cctggcgcgc tacgaggcga gccagatggg gagccgggtc
     1921 atcggctccg agcatctgct cctcgggctc ctcaaggatg gcgacgagct ggtccaggag
     1981 ctgttcgcca aggcggcggt caacatcgag ctgctgcgcg ccgagatgga ggcgcgcggt
     2041 cccagcggcg agaagcaggt caccccgatc gagatcccct tcagcgagga ggcgaagaag
     2101 gtcctcgcct gcgccgagga ggaggcggag cgcctgctcc acccgcacgt cagcgacgag
     2161 cacatcctgc tcggcctgct gcgggtcgag gagtcggcgg ccgggcgcct gctggcc
//
LOCUS       DASRIQ010000346         7652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_668517, whole genome shotgun sequence.
ACCESSION   DASRIQ010000346 DASRIQ010000000
VERSION     DASRIQ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668517"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFD06_08255"
     CDS             complement(<1..280)
                     /locus_tag="VFD06_08255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN03569.1"
                     /translation="MSLGPLASYSHYFWIVGLFLVVDALKVLVEIWNRPVRRSFTSDL
                     HNVTALIPTHNGAKVLRETLNDLLKAGLAHDRILVIDDGSTDDTPKLLA"
     gene            complement(372..908)
                     /locus_tag="VFD06_08260"
     CDS             complement(372..908)
                     /locus_tag="VFD06_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03570.1"
                     /translation="MATCEPALVARARHAYEGGRLTSAAVRTLALVPLPAVALGCGCQ
                     PRTVVIAGSIFLAAVGFCFWRGGDWRRGAIPGIAAGLVPLLTPAALQAGAHACRVDHC
                     GLMTIACAAGGLLGGVLLAWLAPAPRSSGGLPFVVACVVAGLAGAIGCLAYGAIGLAV
                     MTAGLAAGSLPALALRKV"
     gene            complement(895..1434)
                     /locus_tag="VFD06_08265"
     CDS             complement(895..1434)
                     /locus_tag="VFD06_08265"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZN03571.1"
                     /translation="MDDGSLSELQGLMGRLADGDRSAFSRVFEVLWPVLRDLTGRHLP
                     PAEAEDAAQEALLRIFRRSAEFDARRSALAWALGIAHWEIRSVRKKRMRRREESMTDG
                     AMDHRSEQATPEEALLVRDTERMLAQALGTLASADLETLRQYRNGERATVAAATFRKR
                     VERALGRLRSAWRMTHGNL"
     gene            complement(1462..1587)
                     /locus_tag="VFD06_08270"
     CDS             complement(1462..1587)
                     /locus_tag="VFD06_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03572.1"
                     /translation="MSVLMRAKRLLPALAVMAVPAAAWAAETVCSCCECCGGCCL"
     gene            1733..2380
                     /locus_tag="VFD06_08275"
     CDS             1733..2380
                     /locus_tag="VFD06_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZN03573.1"
                     /translation="MDFLHGIVDLFLHLDRHLGEILNTYGAWTYAILALVVFLETGLV
                     ATPFLPGDSLLFAAGAFAALGSLDLWVLLVLLSAAAIAGDSLNYWIGRRVGPRVFTDR
                     VRWLKREHLVRTQEFYERHGGKTIVLARFVPIVRTFAPFVAGIGAMPYRRFFAYNVLG
                     GLLWVGLLVPAGFYFGNLPVVRENFSLVILAIIGVSLLPLVIEYIRQRRAAVPPA"
     gene            complement(2334..3110)
                     /locus_tag="VFD06_08280"
     CDS             complement(2334..3110)
                     /locus_tag="VFD06_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN03574.1"
                     /translation="MSGRDKVIVVLPAYNAARTLERTCADIPAGSADEIILVDDASHD
                     DTVQVARRLGLTVVTHPRNLGYGGNQKTCYQAALERGAGIVVMLHPDYQYPPQILPAM
                     VEMVRSGLFDVVLGSRILGRGALAGGMPLYKYAGNRILTLVENALVGRKLSEYHTGYR
                     AWSRRVLETLPLLENSDGFVFDNQMLCQAFAFGFAIGEVSSPCRYFPEASSIGFGSSV
                     GYGFGVLGTGLEYRLDRWGLRRSPRFRPEGRRLASAGCTR"
     gene            3167..5113
                     /locus_tag="VFD06_08285"
     CDS             3167..5113
                     /locus_tag="VFD06_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03575.1"
                     /translation="MSSLPRPFAFSLVVLAAAAVHLQTLGFPFVFDDRYLVVLNAFLR
                     EAWSPIDAFAHHFWYGTPSGTAYYRPLVIASLALNGKLFGWGPAGFHLFNLLLHAANS
                     ALVLAVLLRLAVPMRAAMFGALLFAVHPVTAWPVASIVARVDLLPAFFLLLAWLALLA
                     GSAPAVGLAFLGALLCKESAVAFLGVPFLALWAPLAPKAPESRPAWRRTLLCGLAAVA
                     ATAAAVALRLFAHAGLGMPRSHIDAVVNPLAAMEDWDRTRAALRLAGRYLAYLFAPWR
                     FTDPAAYHAGHPAPPLTDPGVILGLLLLATLGGLTLWLWLRRDRLGLFLAFALAAFLP
                     ASNLLMPIGSLYAQNFLYMPLLGLALAAGDALGRIEHRLGRVFSYAATAGLVLLSAAT
                     VIEARIWRSGPAVFSAWTERFPNYGFGWAGLGTALLDEGDAAGAERVLRRALALDDGS
                     AKAHYNLGVAILMTTPPPEAGGAGAGTARLQDGLEHSRRAAAIEPVMSEARINAARLL
                     LLLGRPEEAEEEARAAVAVQPDLTARQTLAESIFRQRRYADAMKAYRELVPLLPGDPN
                     LRAQYLESLVEAGDPATARRDAEIARRDFPDLAWFDVCLARIEAREGHADAARDLLRM
                     ARARDPQANDWIGRFRELDSLASN"
     gene            complement(5139..6116)
                     /locus_tag="VFD06_08290"
     CDS             complement(5139..6116)
                     /locus_tag="VFD06_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZN03576.1"
                     /translation="MSRVVRIHSTGGPEVLRLETIDPGRPGQDEVLLRQTAIGVNFID
                     TYHRSGLYPLPSLPHALGQEAVGVVEEVGPGVTGLEKGMRVAYAAGTPGGYAERRVVP
                     AARLIPLPDEIDDRTAAGMMLRGMTAEYLIRRTFKVETGMTVLFHAAAGGVGAIACQW
                     LKHLGAKVIGTVGSDAKAERAKQEGCDEVIVYTRETFPERVREITGGKGVPVVYDSVG
                     KATFEGSLDCLARRGMLVSFGNSSGAPPPLDPLVLSRKGSLYLTRPTLFDYTAKREEL
                     LESARALFDVVRRGAVRVTVGQTFPLAEAAAAHRALESRATVGSTVLVP"
     gene            6177..6860
                     /locus_tag="VFD06_08295"
     CDS             6177..6860
                     /locus_tag="VFD06_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN03577.1"
                     /translation="MPELPRAVEIETGGTVRSSIVWLHGLGADGHDFEPVVPHLRLQD
                     RGVRFVFPHAPRRAVTINMGLLMPAWFDVRSQDFGANIDDAGIRASVGQVEAIVAREK
                     ERGVAAERILLAGFSQGGVIALHTALRHPERLAGILALSTFLPQDSSAQDELSAANAG
                     LPIFMGPGDEAPMIPIAWAEAARDRLEGIGHPVQWKSYPMGHQVCAEEIEDIGAWIRE
                     RLGSLPAGT"
     gene            complement(6843..>7652)
                     /locus_tag="VFD06_08300"
     CDS             complement(6843..>7652)
                     /locus_tag="VFD06_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HZN03578.1"
                     /translation="PRPRILPFDRLDDTMKRRDAMRALAVGGIASLTAAGDALMSHQE
                     SSPSPGSIPSAPPGVPPGRHIAKPLPFDPTKLRGLSERLIASHHENNYAGALKNLNRV
                     EEELQRVTRDTPGFVVSGLKERELLFTNSVILHEFYFGNLGGEGKSGGSIQKSLAEAH
                     GTFARWEELFRATGLSLAGGSGWALLDFNFHTRDLRTYWAGNHTQALAFGYPLLVMDM
                     YEHAYQIDYGAAAARYVDAFFQNINWDEANRRLEGARAAARALAGGSGPGG"
BASE COUNT         1041 a         2772 c         2671 g         1168 t
ORIGIN      
        1 cggcgaggag cttcggcgtg tcgtccgtcg agccgtcgtc gatgacgagg atccgatcgt
       61 gcgcgaggcc cgccttgagc aggtcgttga gcgtctcccg gaggaccttc gccccgttgt
      121 gggtcgggat gagcgccgtc acgttgtgga ggtcggaggt gaacgagcgc cggaccggcc
      181 ggttccagat ctcgacgagc accttgaggg cgtcgacgac caggaagagg ccgacgatcc
      241 agaagtaatg ggagtaggag gcgagggggc cgagactcac gcccggggaa tatagacgat
      301 ctccgcctcc gaccactatc gacctcgcgc tccgcggcgc ggatcacgat cttgaaaccg
      361 gttcgcgcct ctcacacctt cctgagggcg agcgccggaa gcgatcctgc cgcgagcccg
      421 gcggtcatca ccgcgaggcc gatggcgccg taggcgagac agccgatcgc ccccgccagc
      481 ccggcgacga cgcacgccac gacgaagggg agcccgccgg atgagcgtgg cgccggggcg
      541 agccacgcga ggaggacccc gccgaggaga cctcccgccg cgcaggcgat cgtcatgagc
      601 ccgcagtgat cgacccggca ggcgtgcgcc cccgcctgga gggcggccgg ggtcaggagc
      661 ggcaccaggc cggcggcgat cccgggaatg gcgccccgcc gccagtcacc gccgcgccag
      721 aagcagaacc cgaccgccgc caggaagatc gacccggcga tcaccaccgt gcgcggctgg
      781 cagccgcagc cgagcgccac cgccggcagc gggaccagag ccagcgtgcg gaccgcggcg
      841 gaggtcagcc gccctccttc gtaggcgtgc cgcgcccggg cgacgagggc cggctcacag
      901 gttgccatgg gtcatcctcc aggcggagcg caggcggccg agcgcgcgct ccacccgctt
      961 gcggaacgtc gccgcggcca ccgtcgccct ctcgccgttc cggtactgtc gcagcgtctc
     1021 gaggtcggcg gaagcgagcg tcccgagggc ttgcgcgagc atccgctcgg tgtcacgcac
     1081 caggagcgcc tcctcggggg tcgcctgctc cgagcggtgg tccatggccc cgtcagtcat
     1141 cgactcctcg cgccggcgca tcctcttctt ccggacgctg cggatctccc agtgggcgat
     1201 cccgagcgcc cacgcgaggg cgctgcggcg cgcgtcgaac tcggccgagc gccggaagat
     1261 cctcaagagc gcctcctgcg ccgcgtcctc cgcctccgcg ggaggaagat gcctgccggt
     1321 caggtcgcgc agcaccggcc acaacacctc gaagacccgg gaaaaggcgg atcgatcccc
     1381 gtccgccagc cggcccatga ggccctggag ttcggagagc gatccgtcgt ccatccttcg
     1441 aatgggaatc caactcccgc cttagaggca acaacctccg cagcactcgc agcaggaaca
     1501 gaccgtctcc gccgcccacg ccgcggccgg caccgccatc accgccagcg ccggcagcag
     1561 ccgcttcgcc cgcatcagca cgctcatcct ctcaccccct tcccgaccgc gagcccggtg
     1621 ctccttcgat cgacgcaccg cacgcggtca cgcccttcat aggcgcggga agggaaagcg
     1681 tgaccatttc cggaaaagag atcgccgcga atcgggtata gtccgccccc tcatggactt
     1741 cctgcacggc atcgtcgacc tgttcctgca cctcgaccgc cacctcggcg aaatcctgaa
     1801 tacctacggg gcgtggacct acgccatcct ggccctggtg gtgttcctcg agaccgggct
     1861 cgtggccacg ccgttcctgc cgggggattc gctcctgttc gctgccgggg cgttcgccgc
     1921 cctcgggtca ctcgatctct gggtcctgct cgtcctcctg agcgcggcgg cgatcgcggg
     1981 ggacagcctg aactactgga tcgggcggcg cgtcggcccg cgcgtcttca ccgaccgggt
     2041 gcgctggctc aagcgggagc acctggtgcg cacgcaggag ttctacgagc gccacggcgg
     2101 gaagaccatc gtgctggcgc gcttcgtccc gatcgtgcgg accttcgccc cgttcgtggc
     2161 cggcatcggg gcgatgccgt accgccggtt cttcgcctac aacgtcctcg gcgggctgct
     2221 gtgggtcggt ctcctggtgc cggccgggtt ctacttcggc aacctgccgg tcgtgcggga
     2281 gaacttctcg ctcgtgatcc tcgccatcat cggcgtgtcg ctgctgccgc tcgtcatcga
     2341 gtacatccgg cagaggcgag ccgccgtccc tccggcctga agcgcggcga acgccggagc
     2401 ccccagcggt ccagccggta ctcgagaccg gtcccgagca ctccgaaccc gtagcctacg
     2461 ctcgacccga agccgatcga cgacgcctcg gggaagtagc gacaggggct gctgacctcc
     2521 ccgatggcga acccgaaggc gaacgcctga cagagcatct ggttgtcgaa gacgaacccg
     2581 tccgagttct cgagaagcgg cagcgtctcg agcacccggc gcgaccaggc ccggtacccg
     2641 gtgtggtact cggacagctt ccgaccgacg agggcgtttt cgacgagcgt caggatccgg
     2701 ttgccggcgt acttgtagag cggcatcccg ccggcgagcg ccccgcggcc gaggatgcgg
     2761 gagccgagga cgacgtcgaa cagtcccgag cgcaccatct cgaccatcgc cgggaggatc
     2821 tgcggcgggt actggtagtc ggggtgcagc atgacgacga tgccggctcc ccgctcgagc
     2881 gcggcctggt agcaggtctt ctggttgccg ccgtagccga ggttccgcgg gtgggtcacg
     2941 accgtgaggc cgaggcgccg cgccacctgc accgtgtcgt cgtggctggc gtcgtccacg
     3001 aggatgatct cgtcggcgct ccccgccggg atgtcggcgc aggtgcgctc gagcgtccgc
     3061 gcggcgttgt aggccggcag caccacgatg accttgtcgc gcccgctcat tcttcgtccc
     3121 cgcacccggt cctcggcccg cgccgggagg cgtattctat gcgccgatgt cctccctgcc
     3181 gcgccccttc gccttctccc tcgtcgtcct ggcggcggcc gccgtccacc tccagaccct
     3241 cggcttcccg ttcgtcttcg acgaccgcta cctcgtggtg ctcaatgcgt tcctgcgcga
     3301 ggcctggagc ccgatcgacg ccttcgcgca ccatttctgg tacggcacgc cgtccggcac
     3361 cgcctactac cggccgctgg tcatcgcgtc cctggcgttg aacggcaagc tgttcggctg
     3421 ggggccggcg gggttccacc tgttcaacct gctcctgcac gcggcgaaca gcgcgctcgt
     3481 gctggcggtc ctgctgcggc tcgcggtgcc gatgcgggcg gcgatgttcg gcgcgctgct
     3541 gttcgcggtc cacccggtca cggcctggcc ggtcgcatcg atcgtggcgc gcgtcgatct
     3601 cctgccggcc ttcttcctgc tgctcgcgtg gctcgcgctt ctggccggga gcgcccccgc
     3661 cgtcgggctg gcgtttctcg gagcgctgct ctgcaaggaa tcggcggtgg cctttctcgg
     3721 cgtgccgttc ctggcgctgt gggcgccgct cgcgccgaag gcgccggaga gccggcccgc
     3781 ctggcggcgg acgctgctct gcggactcgc cgccgtcgcg gcgacggcgg cggcggtcgc
     3841 gctccgcctg ttcgcgcacg ccggcctcgg catgccgcgc tctcacatcg acgccgtcgt
     3901 gaatccgctc gcggcgatgg aggactggga tcggacgcgg gcggcgctgc gtctcgccgg
     3961 gcgctatctc gcctacctct tcgcgccatg gcgcttcacc gaccccgctg cctaccatgc
     4021 ggggcatccc gcgcctccgc tcaccgatcc gggagtgatc ctcggcctgc tcctgctcgc
     4081 gacgctcggc ggactcaccc tgtggctgtg gctgcggcgc gaccgcctcg gcctcttcct
     4141 cgccttcgcg ctggcggcgt tcctcccggc gtcgaatctc ctgatgccga tcggctccct
     4201 ttacgcgcag aacttcctct acatgccgct gctcggcctg gcgctcgcgg ccggggacgc
     4261 gctggggcgg atcgagcacc ggctcggccg cgtcttctcg tacgctgcga ccgccgggct
     4321 cgtcctgctg tcggcggcga cggtgatcga agcgcgcatc tggagatcgg ggccggcggt
     4381 gttctccgct tggaccgagc gcttcccgaa ctacggtttc ggctgggcgg gattggggac
     4441 ggcgcttctc gacgaggggg acgccgccgg ggcggagcgg gtgctgcggc gggcgctcgc
     4501 cctcgacgac ggcagcgcca aggcgcacta caacctcggc gtcgccatcc tgatgaccac
     4561 gccccctcca gaggcgggcg gcgccggtgc cggcacggcc cgcctgcagg atgggctgga
     4621 gcactcccgg cgcgccgccg ccatcgaacc ggtgatgtcc gaggcgcgca tcaatgcggc
     4681 gcgcctgctg ctcctgctcg gccgtccgga ggaggcggag gaggaggcgc gcgccgccgt
     4741 ggcggtccag ccggacctga ccgcccgcca gacgctcgcc gagtcgatct tccggcagcg
     4801 gcgctacgcc gacgcgatga aggcgtaccg ggagctggtg ccgctccttc ccggcgatcc
     4861 gaacctccgc gcgcagtatc tcgagtcgct cgtcgaggcg ggggacccgg cgaccgcccg
     4921 gcgcgacgcc gagatcgcgc ggcgggactt cccggacctc gcctggttcg acgtctgcct
     4981 cgcgcgcatc gaggcgcggg aggggcacgc cgacgccgcg cgcgatctcc tgcgcatggc
     5041 gcgcgcgcgt gatccgcagg cgaacgattg gatcggacgc ttccgcgagc tggactcgtt
     5101 ggcgagcaac tgagaccgga gcgggagcct gcgggctctc agggcacgag gacggtcgat
     5161 ccgacggtgg cgcgcgactc gagggcgcgg tgcgcggcgg cggcttcggc gagcgggaag
     5221 gtctgcccca cggtgacgcg caccgctccg cggcgcacca cgtcaaacag ggcccgcgcg
     5281 ctctcgagca gctcctcgcg cttcgcggtg taatcgaaga gggtcgggcg cgtcaggtag
     5341 agcgacccct tgcgcgacag gacgagtggg tcgagcggcg gcggggcccc cgacgagttg
     5401 ccgaacgaca ccagcatgcc gcgccgcgcc aggcagtcga gcgacccctc gaaagtcgcc
     5461 ttgccgaccg agtcgtagac gaccggcact cccttcccgc cggtgatctc ccgcacccgc
     5521 tccgggaacg tctcgcgcgt gtagacgatc acctcgtcgc atccctcctg cttcgcgcgc
     5581 tccgccttgg cgtcgctccc gaccgtgccg atcaccttcg ccccgaggtg cttcagccac
     5641 tggcaggcga tggcgccgac gccgcccgcg gcggcgtgga acagcaccgt catcccggtt
     5701 tccaccttga acgtgcggcg gatgaggtac tcggcggtca tgcctcggag catcatgccg
     5761 gcggcggtgc ggtcgtcgat ctcgtcggga agcgggatca gccgcgccgc cggcaccacc
     5821 cgccgctcgg cgtatccccc cggcgtcccg gcggcgtagg ccacgcgcat ccccttctcg
     5881 aggccggtca cgccggggcc gacctcctcg acgacgccta ccgcctcctg gccgagcgcg
     5941 tgcggcagcg acggcagcgg gtagagtccg gagcggtggt aggtgtcgat gaagttgacg
     6001 ccgatggcgg tctgacgcag cagcacctcg tcctgaccgg gccggccggg gtcgatcgtc
     6061 tcgaggcgca gcacctcggg gccgccggtc gagtggatgc ggacgacgcg cgacatgaag
     6121 gcggcatgat acacgcggcg ccggaagccg cgcggagacc tgctatcctg cgccccatgc
     6181 ccgagctgcc gcgcgccgtc gagatcgaga ccggcgggac ggtccggagc tccatcgtgt
     6241 ggctgcacgg tctcggcgcc gacggccacg acttcgagcc ggtcgtgccg cacctgcgcc
     6301 ttcaggaccg cggcgtccgc ttcgtattcc cgcacgcgcc gcgccgggcg gtgacgatca
     6361 acatggggct cctgatgccg gcctggttcg acgtccgatc gcaggacttc ggggcgaaca
     6421 tcgacgacgc cgggatccgc gcctcggtcg ggcaggtcga ggcgatcgtg gcgcgcgaga
     6481 aggagcgcgg cgtcgcggcg gagcggatcc tcctcgccgg cttcagccag gggggagtga
     6541 tcgccctgca cacggcgctg cggcaccccg agcggctggc cggcatcctc gcactgtcca
     6601 cgttcctgcc gcaggattcg tcggcccagg atgagttgtc ggccgccaac gccggcctgc
     6661 cgatcttcat ggggcccggc gacgaggctc cgatgatccc gatcgcctgg gccgaggcgg
     6721 cgcgcgatcg gctcgagggg atcggccacc cggtccagtg gaagtcctac ccgatgggcc
     6781 accaggtctg cgccgaggag atcgaagaca tcggcgcgtg gatccgcgag cgactcgggt
     6841 cgttacccgc cgggacctga tccgccggcg agcgccctcg ccgcagcgcg cgcaccctcc
     6901 agccgccgat tcgcctcgtc ccagttgatg ttctggaaga aggcatccac gtacctggcc
     6961 gccgcggctc cgtaatcgat ctgataggcg tgctcgtaca tgtccatgac gagcagcggg
     7021 taaccgaagg caagcgcctg cgtgtggttg ccggcccagt aggtgcggag gtcgcgcgtg
     7081 tggaaattga agtcgaggag ggcccagccg ctcccacccg cgaggctcag gcccgtcgcc
     7141 cggaacagct cttcccagcg cgcgaatgtt ccatgcgcct cggcgaggct tttctggatc
     7201 gagccgccgc tcttgccctc ccctccgagg ttcccgaagt agaactcgtg gaggatgacc
     7261 gagttcgtga acaggagctc ccgctccttg aggccgctca ccacgaaacc gggagtgtcc
     7321 ctggtgacgc gctggagctc ctcctcgacg cggttcagat tcttgagcgc tccggcatag
     7381 ttgttctcgt ggtgcgaagc gatgagacgc tcggacaggc cacgaagctt cgtggggtcg
     7441 aacggcaggg gcttcgcgat gtgccgtccc ggaggcacgc cgggcggtgc cgatgggatc
     7501 gagcccggag agggggagct ctcctggtgc gacatcagcg catctcccgc tgcagtgagc
     7561 gaggcgatgc cgccgaccgc gagtgcgcgc atggcgtccc gtctcttcat ggtgtcgtcc
     7621 aggcgatcga aagggaggat acgcggccgg gg
//
LOCUS       DASRIQ010000347         5624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_668863, whole genome shotgun sequence.
ACCESSION   DASRIQ010000347 DASRIQ010000000
VERSION     DASRIQ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668863"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1296)
                     /locus_tag="VFD06_08305"
     CDS             complement(187..1296)
                     /locus_tag="VFD06_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03579.1"
                     /translation="MPERWAFYWHGYAFLDSNRQGGDSGARVFESVNHLMLGADRPIG
                     GRWRMGLRGMFSIEPATIPIEGSPLLFQRGETYGGDLLVDRQHAHDLFVMLAAEWERS
                     LGTTGRLLFTAGLRGEPAVGPVAFPHRPSAAEFPMAPLSHHNQDSTHISDDVVSALWG
                     NRVVSVEGSLFHGAEPDEDRWDLDQGSLDSYAGRVTLTPGGGFSLQVSACRREEPEAL
                     EEGNQTRQTASLAWEKTFDGGSSLAVLAGSGRNLLPEDQEEWGHLLEATWHPRPRHFV
                     YGRIEQVDRDLYELTNKTQRPEDVEPKRVHAEQLTLGYAYSWPLLKEAETAAGAAVQG
                     YRFDGRLDDVYGERPISFWVYLRFRFGSGGEHHHH"
     gene            1436..1759
                     /locus_tag="VFD06_08310"
     CDS             1436..1759
                     /locus_tag="VFD06_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN03580.1"
                     /translation="MTISRRSAIAATAALAVLPAAVLAAEKKTDKKEEAMPESEPFGR
                     FTVEQVERRLGRPGVLVVDGNSDETYHEAHIPGAVRLRSKTMTAADLPPDKDATLIFY
                     CHNLL"
     gene            1760..1885
                     /locus_tag="VFD06_08315"
     CDS             1760..1885
                     /locus_tag="VFD06_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03581.1"
                     /translation="MECHRAARRAVEFGYREVFIMPDGIDGWVKAGKKHSKGDTA"
     gene            complement(1895..2932)
                     /locus_tag="VFD06_08320"
     CDS             complement(1895..2932)
                     /locus_tag="VFD06_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03582.1"
                     /translation="MDEVVAPPVPPHDPLAPPAPPTYRDRSGLLLFFGIVEILIGGLC
                     LLLVAASFLVLTAALQTAPGSPQPPALGLWLNVLVYLGAGGFLITMGIGTLRGKKWAR
                     AMMLVTSWPFLLAFLLGVLAAAFVAPSLMEAISSTQGKPEIRAAIWVVMAIMSLFFAA
                     IPLSFILFYSGRNVRATFEARDPGPSWVDGRPLPILGFAGAVGLYGLFLLLGLARGAY
                     GFFGVMLTGWAAALVIVAHVLLCVWLAVQIYRMTRAGWWANVAFAVLVHLSGWITLRV
                     IGIHRLMEASGVSADAGQRPVLFDWVARFTEWFMPASMIVWVLTLVWLRRYYPPGTFA
                     RPAARVARAGN"
     gene            complement(2955..4229)
                     /locus_tag="VFD06_08325"
     CDS             complement(2955..4229)
                     /locus_tag="VFD06_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZN03583.1"
                     /translation="MTEQPRVLLLLPTTTYKAADFLAAAERLGVEAIVGSEQRQALED
                     IAPGRTLTLGLHDAAKAARQIAWAGSARPFAAVVPTDDLTAVVAAHASELLGLPHNSY
                     EAAHAARRKDRLRGRLTAAGVRTPSHRVIRCAPLLLSDADLASEAAAERYPCVLKPVS
                     LAASRGVIRADNPDQFAAAFRRIEAILKRPDVVEKVEPEARSLILVEEYVDGMEVALE
                     GILSRGRLRTLAIFDKPDPLEGPFFEETIYVTPSRLREAEQRAIEVAVAASARALNLQ
                     EGPVHAELRLAPGELPWLIELAARSIGGLCSRVLRFGAGVSLEEVILMHALGTDIDEI
                     ERERRAAGVLMLPIPRSGRLAAVRGLEEARALAGVEEVTLTATIGQPVEALPEGSSYL
                     GFVFARAATPAEAEAALRAAQARIEAEIEPVT"
     gene            complement(4315..5589)
                     /locus_tag="VFD06_08330"
     CDS             complement(4315..5589)
                     /locus_tag="VFD06_08330"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HZN03584.1"
                     /translation="MSITDNRTGKAYEIPIADGTIRAMDLRQIKTGPDDFGIMSYDPA
                     FTNTAACRSAITEIDGDRGILNYRGYPIEQLAEESTYLETAYLILHGELPSRSQYEDW
                     VHNITHHTILHESVKKFIDGFRYDAHPMGMLISTVAAMSTFYKDAKDIFNPKVRREHS
                     YRLLAKVPTLAAFAYRHSTGLPFAYPDNDLSYTGNFLNMLFKMTEVRYKPNPILERAL
                     DVLFILHADHEQNCSASAMRGVGSSQVDPYSAVAAAVAALYGPLHGGANEMVLRMLTE
                     IGSVTRVPDFVKRVKSGETRLMGFGHRVYKNYDPRAKIIKRMADQVFEVTGRNPLLDI
                     ALELERIALQDEYFVSRRLYPNVDFYSGLIYQSMRFPVDMFPVLFAIPRTVGWLAQWE
                     EMLLDPEQKIARPRQIYTGRERREYVPMERRG"
BASE COUNT          907 a         1928 c         1998 g          791 t
ORIGIN      
        1 gggagccatc accgccgcca tggccgccat gaggatcgat cccgccaacc ccgccgtccc
       61 gcgtcgttcg ttcacgattc ctccccgagc acctgcgcca gaagcgccga aaggcgctcg
      121 agtgtatacg gcttcggcag catccctgcg aagccgtgct cttcgtaccg cgccatcacc
      181 gggtcgtcag tggtggtggt gctctcctcc cgacccgaag cggaagcgca gatagaccca
      241 gaaggagatc ggccgctcgc cgtacacgtc gtcgaggcga ccgtcgaagc gatatccctg
      301 caccgcggca cctgccgccg tctcggcctc tttcaggagc ggccaggagt aagcgtaccc
      361 gagtgtcagc tgttcggcat gcacgcgctt cggctcgaca tcttccggcc gctgcgtctt
      421 gttggtcagc tcatagaggt cgcggtccac ctgctcgatg cgcccgtaga cgaagtgccg
      481 cgggcgcggg tgccacgtgg cctcaagcag gtgcccccac tcctcctggt cctcgggaag
      541 aaggttgcgg ccgctcccgg cgaggacggc gagggagctg ccgccgtcga acgtcttctc
      601 ccatgccagc gacgccgtct gccgcgtctg attcccctct tcgagcgcct ccggctcctc
      661 gcgccggcag gcggacacct gcaggctgaa gccgcccccg ggcgtgagcg tgacgcggcc
      721 ggcgtaggag tcgaggctcc cctggtcgag gtcccagcgg tcctcgtccg gctccgcgcc
      781 gtggaagagg gacccctcca ccgacaccac ccggttcccc cacagtgccg agacgacgtc
      841 gtcggagatg tgggtcgagt cctggttgtg gtgcgacagg ggcgccatcg ggaactcggc
      901 cgcggagggg cgatgcggga acgccaccgg cccgaccgcc ggctcgccac gcagccccgc
      961 cgtgaacagc agtcgaccgg tcgtgccgag cgagcgctcc cactcggccg ccagcatcac
     1021 gaacaggtcg tgcgcgtgct gtcgatcgac cagcagatcg ccgccgtacg tctcaccccg
     1081 ctggaagaga agcgggcttc cctcgatcgg gatcgtcgcc ggctcgatgg agaacatgcc
     1141 gcgcagcccc atccgccagc ggccgccgat cggccggtcc gccccgagca tcaggtggtt
     1201 gaccgattcg aagacgcgcg cgcccgagtc gcccccctgg cgattggagt cgaggaaggc
     1261 gtacccgtgc cagtagaacg cccagcgctc aggcacagac ggtggatcgg cggtctgggc
     1321 caaggcgatc ggagcggcgc aggcgcacag tgcgaaagcc gcgaggcggg cgtagaatct
     1381 ccgcattctc ccggcgcgcg aagcaccgga tgccggagga gaggagggcg cccccatgac
     1441 catctcccgc aggtccgcca tcgcggccac cgccgcgctc gcggtactcc ccgctgccgt
     1501 cctggcggcg gaaaagaaga ccgacaagaa agaagaagcg atgccggagt cggaaccgtt
     1561 cgggcgcttc accgtcgaac aggtcgagcg ccgcctgggc cggcccggcg tgctcgtcgt
     1621 cgacggcaac agcgacgaaa cctatcacga agcccacatc ccgggggccg tgcggctgcg
     1681 cagcaaaacg atgacggccg cggaccttcc gcccgacaag gacgccacgc tcatcttcta
     1741 ctgccacaac ctgctttgaa tggagtgcca tcgcgcggcc cggcgggccg tggagttcgg
     1801 ttaccgcgag gtcttcatca tgccggacgg gatcgacggc tgggtcaagg cgggcaagaa
     1861 gcacagcaag ggggacacgg cctagccgcc gccttcaatt ccccgcgcgt gcgactcgcg
     1921 cggcaggccg cgcgaacgtc cccggggggt agtaccggcg cagccagacg agcgtcagca
     1981 cccagacgat catcgaggcc ggcatgaacc actcggtgaa tcgggcgacc cagtcgaaga
     2041 gaacgggtcg ctgccccgcg tcggcagaga cccctgacgc ttccatgagc cggtggatgc
     2101 cgatgacccg cagggtgatc cagcccgaga ggtgcacgag cacggcgaac gcgacattgg
     2161 cccaccagcc ggcccgcgtc atccggtaga tctgcacggc gagccagacg cacagaagca
     2221 cgtgcgcgac gatcaccagg gcggccgccc aacctgtgag catgactccg aagaacccgt
     2281 acgctccgcg cgccagcccc agcagcagga acagcccata aagcccgacc gcaccggcga
     2341 acccgaggat cggcagcggg cgtccatcca cccaggacgg ccccgggtca cgcgcctcga
     2401 aggtggcgcg gacgttgcgc ccgctgtaga acaggatgaa cgagagcggg atcgcggcga
     2461 agaagagcga catgatcgcc atgacgaccc agatcgccgc gcgaatttcg ggcttcccct
     2521 gggtcgagga gatcgcttcc atgagagacg gagcgacgaa cgccgccgcg agaacgccga
     2581 gcaggaaggc gaggaggaac ggccacgacg tgacgagcat catggcccgc gcccacttct
     2641 tcccgcgaag cgtgccgatc cccatcgtga tgaggaagcc gccggcgccg aggtagacca
     2701 ggacgttgag ccagagtccg agggccgggg gttgcgggct gccgggagcc gtctgcagtg
     2761 ccgcggtcag cacgaggaac gaggcggcca ccagcagcag gcacagcccg ccgatcagga
     2821 tctcgacgat tccgaagaag agcagcaggc cgctccggtc gcggtaggtc ggcggggcgg
     2881 ggggagcgag tggatcgtgc ggcgggacgg gcggggcgac gacctcgtcc atgcggttcc
     2941 ctcctgcccg gcgatcaggt gaccggttcg atctccgctt cgatacgggc ctgggcggcg
     3001 cgcagcgccg cctccgcctc ggccggggtg gcggcgcgcg cgaagacgaa gccgaggtag
     3061 gacgacccct cggggagcgc ctcgaccggc tggccgatgg tggcggtgag ggtgacctcc
     3121 tcgacgccgg ccagggcgcg cgcctcctcg aggccgcgca ccgcggcgag ccggccggag
     3181 cgcgggatcg gcagcatcag caccccggcg gcgcgccgct cgcgctcgat ctcgtcgatg
     3241 tcggtgccga gggcgtgcat caggatcacc tcttcgagcg acacgccggc gccgaagcgc
     3301 aggacccggg agcacaggcc gccgatggag cgggccgcca gctcgatgag ccagggcaac
     3361 tcccccggcg cgagacgcag ctcggcatgc accggcccct cctgcaggtt cagggcgcgc
     3421 gccgaggcgg cgaccgccac ctcgatcgcc cgctgctcgg cctcgcgcag gcgcgacggc
     3481 gtcacgtaga tcgtctcctc gaagaacggc ccttcgagcg ggtccggctt gtcgaagatc
     3541 gccagcgtgc gcaggcggcc gcgggagagg atcccttcga gcgcgacctc cattccgtcc
     3601 acgtactcct cgaccaggat gagactgcgc gcctccggtt cgaccttctc cacgacgtcg
     3661 ggacgcttga ggatcgcctc gatccggcgg aaggcggcgg cgaactggtc cgggttgtcg
     3721 gcgcggatga ccccgcggct ggcggcgagc gacaccggct tgaggacgca cgggtagcgc
     3781 tcggccgcag cctccgaagc caggtccgcg tctgacagga gaagcggcgc gcaccggatg
     3841 acccgatggg agggagtgcg gacaccggcg gcggtcaagc ggccgcgcag acggtccttg
     3901 cgccgcgcgg cgtgggccgc ctcgtacgag ttgtgcggca ggccgagcag ctccgaagca
     3961 tgcgccgcca ccacggcggt gagatcgtcg gtcggcacca ccgcggcgaa cggccgggcg
     4021 ctcccggccc aggcgatctg gcgcgccgcc ttggcggcgt cgtgcaggcc gagagtcagc
     4081 gtcctgccgg gggcgatgtc ctccagcgcc tggcgctgct ccgagccgac gatcgcctcg
     4141 acgccgaggc gctctgccgc cgccaggaag tcggccgcct tgtaggtggt ggtcgggagc
     4201 aggaggagaa cgcgcggttg ttcggtcacg ggggtcaagc ggccctgccg cgcggcacgg
     4261 cgcggctcct gtccggttcg cggactcagc cgcggcgttt cgtcaggcga ggcatcagcc
     4321 gcgccgctcc atcggcacgt actcgcggcg ctcgcggccg gtgtagatct ggcgcggccg
     4381 ggcgatcttc tgctcggggt cgaggagcat ctcctcccac tgcgccagcc accccaccgt
     4441 gcgcgggatg gcgaacagca ccgggaacat gtccaccggg aagcgcatgc tctggtagat
     4501 gagcccggag tagaaatcga cgttcgggta cagccgccgg ctgacgaagt actcgtcctg
     4561 gagcgcgatc cgctccagct ccagcgcgat gtcgaggagc gggttgcgcc cggtcacctc
     4621 gaacacctgg tcggccatcc gcttgatgat cttggcgcgc ggatcgtagt tcttgtagac
     4681 ccggtgcccg aagcccatca gccgcgtctc gcccgacttg acgcgcttga cgaagtcggg
     4741 gacgcgcgtc accgagccga tctcggtcaa catgcgcagc accatttcgt tggcgccgcc
     4801 gtggagcggg ccgtacaggg ccgcgaccgc cgccgccacc gcggagtacg ggtccacctg
     4861 ggacgacccg acgccgcgca tggcgctggc agagcagttc tgctcgtggt cggcgtgcag
     4921 gatgaacagg acgtcgaggg cccgctcgag gatcgggttc ggcttgtagc gcacctcggt
     4981 catcttgaag agcatgttca ggaagttgcc ggtgtacgac aggtcgttgt ccgggtacgc
     5041 gaacggcagc ccggtcgaat ggcggtaggc gaacgccgcg agcgtcggca ccttcgcgag
     5101 cagccggtag gagtgctcgc gccgcacctt ggggttgaag atgtccttgg cgtccttgta
     5161 gaacgtggac atcgcggcga ccgtcgagat cagcatcccc atcgggtgcg cgtcgtagcg
     5221 gaacccgtcg atgaacttct tgacgctctc gtggaggatg gtgtggtgcg tgatgttgtg
     5281 cacccagtcc tcgtactgcg accggctcgg cagctcgccg tgcaggatca ggtaggcggt
     5341 ctcgaggtag gtgctctcct ccgccagctg ctcgatcggg tagccgcggt agttgaggat
     5401 gccgcggtcg ccgtcgatct cggtgatggc gctgcggcag gcggcggtgt tggtgaacgc
     5461 cgggtcgtac gacatgatgc cgaagtcgtc cggccccgtc ttgatctgcc gcagatccat
     5521 ggcgcggatg gtgccgtccg cgatcgggat ctcgtacgcc ttgccggtgc ggttgtcggt
     5581 gatcgacagg gtggaggtgg cgcgctccgg ggccgcggcc gggc
//
LOCUS       DASRIQ010000348         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1960157, whole genome shotgun sequence.
ACCESSION   DASRIQ010000348 DASRIQ010000000
VERSION     DASRIQ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960157"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VFD06_08335"
     CDS             complement(<1..869)
                     /locus_tag="VFD06_08335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZN03585.1"
                     /translation="MIGGVARAAGMEGAMSQSFFDKTLITEREEVDSQRWKKHHACLV
                     VMQGAEIGRDFRLRRGSMTIGRGLDVDIRIPDDLASRRHARVDFNWDPFKETATFVLS
                     DLESTNHTFVNSRQVDRIELQDGDKIQIGDTVLKFVVLDEIEVKFHAEVRNRITFDQL
                     SGLLTKESLFLALEMELKRCHRYTVPLAVLMMDLDHFKQVNDTRGHLMGSHVLAEVGR
                     LIARSIRGADVAARFGGEEFVAYLPEADGAGAVQVAERIRLSIESTEFTLDGETVRTT
                     ISAGVALFPAHGN"
     gene            1098..2084
                     /locus_tag="VFD06_08340"
     CDS             1098..2084
                     /locus_tag="VFD06_08340"
                     /inference="COORDINATES: protein motif:HMM:NF041079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBASS cGAMP-activated phospholipase"
                     /protein_id="HZN03586.1"
                     /translation="MLPRSEPIEPAFRILTLDGGGIRGIFTASFLATAEEMAGRRLGD
                     HFDLIAGTSTGGIIALAVAFGIPARRVLEMYVERGKRIFRRPRRLGMLVRPRYGNRDL
                     TRALRDIFGDRTINDVKTPVCIPSYELTNSYPRVWKDEHAGNLLWWGDQPAWRIALAT
                     SAAPLYFPAFQVLPGDSHVDGGLYANNPVLVAVTEAVHTFAQPLERIRVLSIGAGERA
                     ERIPFERASGMGLWQWRSAIYEHMLIAQARSAHEIARRLLAPGQYERVNIPLEHACRL
                     DDYEAATTLLEPGAQEARTRSELLRDQFLDGPAWMGRDQKEAVAATYRRGAA"
     gene            2098..>2372
                     /locus_tag="VFD06_08345"
     CDS             2098..>2372
                     /locus_tag="VFD06_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03587.1"
                     /translation="MPARKPKSPSRQSKSPSRQAEKRSDPGPAPRGGPRSEAALARVR
                     SVCLALPDTTEKIAWGAPTFRVRERLFVMFSDNHHGDGRLAIWCSAAP"
BASE COUNT          355 a          857 c          811 g          349 t
ORIGIN      
        1 tcgttcccgt gcgccgggaa gagagcgacg ccggcgctga tggtggtgcg gaccgtctcg
       61 ccgtcgagcg tgaactcggt cgattcgatg ctcaggcgga tgcgctccgc cacctgcacc
      121 gccccggccc cgtcggcctc aggcaggtaa gcgacgaact cctccccgcc gaagcgcgcc
      181 gccacgtcgg cgccgcggat cgagcgggcg atgagccggc cgacctccgc caggacgtgg
      241 ctccccatca ggtggccgcg cgtgtcgttc acctgcttga aatgatcgag gtccatcatc
      301 aggaccgcca gcggcacggt gtagcggtgg cagcgcttca gctccatctc cagcgccagg
      361 aacagcgact ccttcgtcag gaggccgctc agctggtcga aggtgatccg gttgcgcacc
      421 tcggcgtgga acttcacctc gatctcgtcg aggaccacga acttcagcac cgtgtcgccg
      481 atctggatct tgtcgccgtc ctgcagctcg atccgatcga cctggcgcga gttcacgaag
      541 gtgtggttgg tgctctcgag atcggacagg acgaaggtgg cggtctcctt gaacgggtcc
      601 cagttgaaat cgacgcgggc gtggcggcgg gaggcgaggt cgtccgggat gcggatgtcg
      661 acgtcgaggc cgcgcccgat cgtcatcgag ccgcgccgca gccggaagtc ccggccgatc
      721 tccgccccct gcatcaccac gaggcaggcg tggtgcttct tccagcgctg gctgtcgacc
      781 tcctcgcgct cggtgatgag ggtcttgtcg aagaagctct gcgacatcgc tccttccatc
      841 ccggccgccc gcgccacccc gccgatcacg atctcgtgat cgacgccgcg cggccgacac
      901 ggtccaatcc ggccccaatc tatgtccggg ctgctccggg cgcatccccc gcggaaagcc
      961 gtgccggacg gggcttgtcg ggcaggcccg tccgattcat actgaccgtg cagagagcga
     1021 agggccgcca ggcccctccg gcatcacccg cagtcgcatc cgcccccagg cacctctcga
     1081 agcaaggcag gctcgacatg cttccgcgaa gcgagccgat cgaaccagca ttccgcatcc
     1141 tgaccctcga cggcggaggg atccggggca tcttcaccgc ctcgttcctc gccaccgccg
     1201 aggagatggc cggtcggcgc ctgggcgatc acttcgacct gatcgccggc acctccaccg
     1261 gcggcatcat cgcgctggcg gtggcgttcg gcatcccggc gcgccgcgtg ctcgagatgt
     1321 acgtcgagcg cgggaagcgc atcttccggc ggccgcgccg gctggggatg ctcgtccggc
     1381 cgcgctacgg caaccgcgac ctgacgcggg cgctgcgcga catcttcggc gaccggacga
     1441 tcaacgacgt gaagacgccg gtctgcatcc cgtcgtacga gctgaccaac tcctacccgc
     1501 gcgtctggaa ggacgagcac gccggcaacc tgctctggtg gggggaccag ccggcgtggc
     1561 gcatcgcgct cgccacctcc gccgcgccgc tctacttccc ggcgttccag gtgctgccgg
     1621 gggacagcca cgtggacggc gggctgtatg ccaacaaccc ggtgctggtc gcggtcaccg
     1681 aggcggtgca caccttcgcg cagccgctcg agcggatccg cgtgctgtcg atcggcgcgg
     1741 gggagcgggc cgagcggatc ccgttcgagc gcgcgtcggg catggggctg tggcaatggc
     1801 gttcggcgat ctacgagcac atgctcatcg cccaggccag aagcgcccac gaaatcgccc
     1861 ggcggctgct cgccccgggg cagtacgagc gcgtcaacat cccgctggag cacgcctgcc
     1921 ggctcgacga ctacgaggcg gccacgaccc tcctcgagcc cggggcccag gaggcgcgca
     1981 cccgcagcga gttgctccgg gatcagttcc tcgacggccc ggcctggatg gggcgggatc
     2041 agaaggaagc ggtcgcggcg acgtaccggc gcggcgcggc gtgagagaat ccgccgcatg
     2101 ccggcccgca agccgaagtc tccgtcccgc cagtcgaagt ctccgtcgcg ccaggcggag
     2161 aagcgatcgg acccggggcc cgcgccgcgc ggcggcccca gaagcgaagc ggcgctcgcc
     2221 cgggtgagga gcgtctgcct ggcgcttccc gacacgacgg agaagatcgc ctggggggcc
     2281 ccgaccttcc gcgtccgcga gcggctcttc gtgatgttct cggacaacca ccacggcgac
     2341 ggccgcctgg cgatctggtg cagcgccgcc cc
//
LOCUS       DASRIQ010000349         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3532139, whole genome shotgun sequence.
ACCESSION   DASRIQ010000349 DASRIQ010000000
VERSION     DASRIQ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3532139"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFD06_08350"
     CDS             <1..382
                     /locus_tag="VFD06_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015125707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZN03588.1"
                     /translation="EAGLPAAGKEMTEDHNPWEARLDDAISLSKGCYVGQEVIARLNT
                     YRKVARLLVRLEAEGGPFPEGAEIAADSLTIGRVTSSAALPDGSGRSLSLGYVRDEDA
                     IAGKPVTVTTPGGPRPAVVAGPAR"
     gene            379..795
                     /locus_tag="VFD06_08355"
     CDS             379..795
                     /locus_tag="VFD06_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HZN03589.1"
                     /translation="MSAGAGKKESVPRTGLLLIGHGSRATEAVKVLADVAASLRRRFR
                     GFVVEPCFLELNQPDIQTAIDRAVGQGATRILFVPFSTYLDGHVGRDLPEHIGVARER
                     HPGLDIRIAPHLGPDPRLVAICADRIRKGLRAGRWT"
     gene            895..1833
                     /gene="trxB"
                     /locus_tag="VFD06_08360"
     CDS             895..1833
                     /gene="trxB"
                     /locus_tag="VFD06_08360"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZN03590.1"
                     /translation="MSKSSDVRDVVILGSGCAGLTAAVYAARGNLKPLLVDGHEAGGQ
                     LSMTTEVENFPGFPEGIQGPQLIEHMRKQAARFGTEFLTGAAHHADLQTRPFSLTLDH
                     NKKLHTKTLIIASGASARLLGIESERRLLGHGVSTCATCDGFFFKEKDLLVVGGGDSA
                     IEEGTFLTKFARQVTIVHRRDTLRASKIMQDRAFKNPKVKFVWDSTVDEVLGKHKVEG
                     ARLKNLKTGAVTEMKVDGVFVAIGHIPNTKIFAGQIDMDQAGYIVTKGGSRTNVPGVF
                     AAGDCQDHVYRQAVTAAGSGCMAAIDAERFLEAEGH"
     gene            1889..2071
                     /locus_tag="VFD06_08365"
     CDS             1889..2071
                     /locus_tag="VFD06_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03591.1"
                     /translation="MQSTVDELLAAIDEAMSLMKGRGAGRYTAEVDNLLRVKKVLERL
                     DPDEVEALLEDEDEEE"
     gene            complement(2126..2211)
                     /locus_tag="VFD06_08370"
     tRNA            complement(2126..2211)
                     /locus_tag="VFD06_08370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2175..2177),aa:Leu,seq:gag)
BASE COUNT          393 a          846 c          880 g          347 t
ORIGIN      
        1 cgaggcgggg ctgccggcgg ccgggaagga gatgaccgag gatcacaacc cctgggaggc
       61 gcgcctcgac gacgccatct ctctctccaa gggctgctac gtggggcagg aggtgatcgc
      121 gcggctcaac acctacagga aagtggcccg gctcctcgtc cgcctcgagg cggaaggggg
      181 gccgtttccc gaaggggccg agatcgcagc cgattccttg accatcggtc gcgtcaccag
      241 cagcgccgcg ctccccgatg gcagcggcag atcactttcg ctcggctatg tgcgcgacga
      301 ggacgcgatc gcggggaagc cggtgacggt cacgaccccc ggcgggcctc ggccggccgt
      361 ggtcgcggga ccggcccgat gagcgcgggc gccggtaaga aggagagcgt cccccgcacc
      421 gggctcctgc tcatcggcca cggaagccgc gccaccgagg cggtcaaggt gctggcggac
      481 gtggcggcgt cgctgcgccg tcgcttccgc ggcttcgtcg tcgaaccgtg cttcctcgag
      541 ctgaaccagc cggacatcca gaccgccatc gatcgggccg tcggccaggg ggcgacgcgc
      601 atcctcttcg tgccgttctc gacctacctc gacgggcacg tcggccgcga tctgccggag
      661 cacatcgggg tggcgcgcga gcgccacccg gggctcgaca tccggatcgc gccgcacctc
      721 gggcccgacc cgcgtctcgt ggcgatctgc gccgaccgga tccgcaaggg cctccgggcc
      781 ggccgttgga cctgacggtc cgatccagtt ccgcgtgcta tcatcgccgc cgttcctcaa
      841 tgcagcgtca cacgcctcgt gcgcgggcca tgcgcgcgtc cccagggaga gatcgtgtcg
      901 aagagcagtg atgtccgtga cgtggtgatc ctcggctccg gctgcgccgg gctgacggcc
      961 gccgtctacg cggcgcgcgg caacctcaag ccgctgctcg tggacgggca cgaggcgggc
     1021 gggcagctga gtatgacgac cgaggtcgag aacttccccg ggttcccgga ggggatccag
     1081 ggtccgcagc tgatcgagca catgcgcaag caggcggcgc gcttcggcac cgagttcctc
     1141 actggcgccg cccaccacgc ggatctccag acgcgcccgt tctcgctgac gctcgaccac
     1201 aacaagaagc tgcacacgaa gactctcatc atcgcgtcgg gggcgtcggc ccgcctgctc
     1261 gggatcgaat cggagcgtcg gctcctgggg catggggtga gcacgtgcgc cacctgcgac
     1321 ggcttcttct tcaaggagaa ggacctcctc gtcgtgggag gcggcgactc ggcgatcgag
     1381 gaggggacct tcctcaccaa gttcgcccgg caggtgacca tcgtgcaccg ccgcgacacg
     1441 ctgcgcgcct cgaagatcat gcaggatcgg gcgttcaaga accccaaggt caaattcgtc
     1501 tgggattcga ccgtcgacga ggtgctcgga aagcacaagg tcgagggggc gcgactgaag
     1561 aacctcaaga cgggcgccgt caccgagatg aaggtggacg gcgtcttcgt ggccatcggc
     1621 cacattccca acacgaagat cttcgccggc cagatcgaca tggaccaggc cggctacatc
     1681 gtcacgaagg ggggaagccg caccaacgtc ccgggcgtgt tcgcggcggg ggattgccag
     1741 gatcacgtct accgccaggc ggtcaccgct gccggaagcg gctgcatggc ggcgatcgac
     1801 gcggagcgct tcctcgaagc cgaaggtcac tgacgtcccg cgccgtggtc cggcgcgaac
     1861 gcgcgcgaga ccgcgcggag gagggagcat gcagagcacc gtcgacgaac tgctcgcggc
     1921 gatcgacgag gcgatgtccc tgatgaaggg ccgcggcgca ggtcgctaca ccgccgaagt
     1981 ggacaacctg ctgcgcgtca agaaggtcct ggagcggctc gacccggacg aggtcgaggc
     2041 gctgctcgaa gacgaggacg aggaagagta ggcgggcccg ccccgttcag ggcgtcgagg
     2101 ttcgttcggg cagggcgggg aaggctggtg ccggaggcgg gatttgaacc cgcatgcctt
     2161 ttgggcgcca ccccctcaag atggtgtgtc tgccaattcc accactccgg cacactgctt
     2221 gactcttcac gatctcagca cgcctgcgag cggggcgttg cggccccgcg gccccacact
     2281 taccggccgc cctcggccgg cttctccggc gcgggggcct gaccggatgg cgccgcagcg
     2341 cctccgtcgg cgggcgcggc cgcaccctcc tgcgccgggg caggagcggg ggtcgccgcc
     2401 ggctccgagg gtaccgcctc ggggctcgcc gtggccggaa gcgggacctg ctcgaggacc
     2461 gacccg
//
LOCUS       DASRIQ010000350         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2213168, whole genome shotgun sequence.
ACCESSION   DASRIQ010000350 DASRIQ010000000
VERSION     DASRIQ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213168"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VFD06_08375"
     CDS             <1..1220
                     /locus_tag="VFD06_08375"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZN03592.1"
                     /translation="LNAELDRGPLADPKRLDALRARMKERMDEHRSLVVRDEFTEIVQ
                     ENGGTGINASTGTDLTSYYVNLPANRIELWCLLEAARLRDPVLREFYSERDVVKEERR
                     FRIDNHPQGKLYEQLLLTAYSAHPYRVPGAGWLSDLDRLTRPEAEEFRRIHYIPGNAV
                     GALVGQIDVEATAALLKRYFGPIPSGPLPLSPATVEPPQTGERRVAAQFDAAPNLMIG
                     FHKSALADPDDPVYEVIDGLLGAGRTGRLFRRLVTGDQVASQVFTFEAPGRRFPSLFV
                     IGGQPRAPHTTAELERGILDELRRLSEEPVPEEELRKVRAQVESDAVYELRGNMGIAS
                     RLTQFAALTGDWRTMLRRDAALLQVTGEQVREVARRTFTAANRTVATLERPVAAAPPG
                     PAGGPAAPGGKER"
     gene            1217..2776
                     /locus_tag="VFD06_08380"
     CDS             1217..2776
                     /locus_tag="VFD06_08380"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZN03593.1"
                     /translation="MRSATALCVAALALSAAASVAPGAAAAAPAADPRELKYPPLEIR
                     FPAPQTKTLGNGLVVHLLPDRDLPIVDLAFFARGGSIFDPDDRAGLAVLALHAMRTGG
                     TSALTPDQVDERLETLPADIDLGADDDSLTGSLSCLKGRFPEALAIFAGMLRSPRFDQ
                     ERLDAERGRFLEQIRRRWDDPGTISHIVFARLVYGEDSPWARLPTSETVGRVGRDDLV
                     ALHRRFFRPGNLRLAVSGDFEPKEMLRLLEKTLGDWRGAAVDLPDVPRIREQANAGVW
                     LVERPLTQSAIALGHLGVNRFDADKFPLSVVNEVLGEGGFTSRLMKEVRSTRGLAYSV
                     SGGVGSDSDRGLFEIRCRTRADATSTAIEAIRDVVRRFRDEGPTEEEVRKAKDGQTNS
                     FVFTVDGTPAYMTQALYYEHYGYPANYLPSWRDRLAAVTREHAARAARAHIKPESMVV
                     LVVGNPDAFDRPLDALGLGAPRRITLPEGGGLPRLSAAAAPSSDAPPASGAAPPGSGG
                     RRAAAPAAPPS"
     gene            complement(2883..3662)
                     /locus_tag="VFD06_08385"
                     /pseudo
     CDS             complement(2883..3662)
                     /locus_tag="VFD06_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT          490 a         1395 c         1270 g          538 t
ORIGIN      
        1 acctgaatgc cgagctggat cgcggcccgc tcgccgaccc gaagcgtctc gacgcgctgc
       61 gggcccggat gaaggagcgg atggacgagc accggagcct cgtggtgcgg gacgagttca
      121 cggagatcgt gcaggagaac ggcggcaccg ggatcaacgc gtcgaccggg actgacctga
      181 cctcgtacta cgtcaacctg cccgcgaacc ggatcgagct gtggtgcctg ctcgaggcgg
      241 cccgcctgcg cgacccggtg ctgcgcgagt tctacagcga gcgggacgtc gtcaaggagg
      301 agcgccgctt ccgcatcgac aaccatccgc aggggaagct gtacgaacag ctgctcctga
      361 cggcgtacag cgcccatccg taccgcgtcc ccggagccgg ctggctctcc gacctcgacc
      421 gcctcacccg ccccgaggcg gaggagttcc gccggatcca ttacatcccc ggcaacgccg
      481 tcggtgccct cgtcggccag atcgacgtcg aggccaccgc cgccctcctg aagcgctact
      541 tcggaccgat cccgtcgggg ccgctgcccc tctcccccgc gaccgtcgag ccgcctcaga
      601 ccggcgagcg ccgcgtcgcg gcgcagttcg acgccgctcc gaacctcatg atcggcttcc
      661 acaagtccgc tctcgccgac ccggacgacc cggtgtacga ggtgatcgac ggactgctcg
      721 gggcgggccg gaccggccgg ttgttccgcc gtctcgtgac cggcgaccag gtcgcgagcc
      781 aggtcttcac cttcgaggcg ccggggcggc gcttcccgtc cctcttcgtg atcggcggcc
      841 agccgcgcgc gccgcacacc accgccgagc tcgagcgcgg catcctcgac gagctgcgcc
      901 gtctctcgga ggagccggtg cccgaggagg agctgcgcaa ggtgcgggcg caggtcgaat
      961 cggacgcggt ctacgagctg cgcggcaaca tggggatcgc ctcgcgcctg acgcagttcg
     1021 ccgccctcac cggcgactgg cgcaccatgc tgcgtcgcga cgcggcgctc ctgcaggtca
     1081 cgggcgagca ggtccgggag gtcgctcgcc ggacgttcac ggcggcgaac cggaccgtcg
     1141 cgacgctgga gcgccccgtg gccgccgcgc cgccgggccc tgccggcggg cccgccgcgc
     1201 ccggaggcaa ggagcgatga gatccgcgac cgccctctgc gtcgcggctc tcgctctctc
     1261 ggctgcggct tcggtcgcgc cgggggcggc cgccgcggcc ccggcggccg acccgcgcga
     1321 gctcaagtat ccgccgctcg agatccgctt cccggcgccg cagaccaaaa cgctcggcaa
     1381 cggcctcgtc gtccatctcc tgccggatcg cgacctgccc atcgtcgatc tggcgttctt
     1441 cgcgcgcggc gggtcgatct tcgacccgga cgatcgcgcc ggcctggcgg tcctcgcgct
     1501 gcacgccatg cgcaccggcg gcacctccgc cctgaccccc gaccaggtgg acgagcgtct
     1561 ggagacgctg ccggcggaca tcgacctcgg cgccgacgac gactccctga ccggcagcct
     1621 ctcctgcctc aagggtcgct ttcccgaagc gctcgcgatc ttcgccggca tgctgcggtc
     1681 gccgcgcttc gaccaggagc gcctggacgc ggagcggggc cggttcctcg agcagatccg
     1741 ccgccggtgg gacgaccccg gcaccatctc gcacatcgtc ttcgcgcggc tggtgtacgg
     1801 cgaggacagt ccgtgggccc gtctgccgac gtccgagacg gtcggccgcg tggggcgcga
     1861 cgatctcgtc gcccttcacc gccgtttctt ccgcccgggg aacctccgcc tcgcggtctc
     1921 gggcgacttc gagccgaagg agatgctccg gctgctcgag aaaacgctcg gcgactggcg
     1981 cggcgccgcc gtcgacctgc cggacgtccc gcgcatccgg gagcaggcga acgccggcgt
     2041 gtggctcgtc gagcgcccgc tgacgcagag cgccatcgcc ctgggtcacc tgggggtcaa
     2101 ccgcttcgac gcggacaagt ttcctctgtc cgtcgtcaac gaggtcctcg gagagggtgg
     2161 gttcacgtcg cgcctcatga aggaggtccg ctcgacgcgc ggcctcgcct actcggtcag
     2221 cggcggcgtc ggatccgaca gcgatcgcgg cctgttcgag atccgctgcc gcacccgcgc
     2281 cgacgccacg tccacggcga tcgaggcgat ccgggacgtg gtgcgccgct tccgcgacga
     2341 aggcccgacc gaggaggagg tccgcaaggc caaggacggc cagaccaact ccttcgtgtt
     2401 caccgtggac ggcaccccgg cctacatgac gcaggcgctc tactacgagc actacggcta
     2461 tcccgccaat tacctcccgt cctggcgcga tcggctcgcc gcggtcacac gcgagcacgc
     2521 cgcacgcgcc gcacgcgctc acatcaagcc ggagtcgatg gtggtgctgg tcgtggggaa
     2581 cccggacgcc ttcgaccggc cgctcgacgc gctcggcctg ggggcgccgc ggcggatcac
     2641 ccttccggaa ggaggggggt tgcctcggct ttcggccgcg gcggcgccgt cttccgacgc
     2701 accgcccgct tccggggccg ctccgccggg atcggggggg cgccgggcag cagcgccggc
     2761 tgctccgcct tcgtgaacgg cccgccgccc cgcgccaggt agcgctcccg gtcgaggttc
     2821 cgcagccgct ccgcggcgac gagctccgcc gccaccttgt ccttctccgc cgggcagatg
     2881 tcgcggaact cgcaccaccg gcacagcggg ctcggccggg ccgggaagtc gcgcgccgcc
     2941 tcgatcctgt ccaccagccg gatcgcctgg cggcggtgct cctcgatctc cgcctcggtg
     3001 cgggtcgcag tgcggcggtc cccctgcgcc aggaagtgcc acaccagccg cacgtcccgc
     3061 gcgtcggcgt gccggtcgcg cacggcgatc tggtagaagg tgagctgccg atcgcgcctc
     3121 agatcctcgt cgctcggccg gaacgcgccg gtcttgtagt cgtggatctc gtactgcccc
     3181 ggcccgcgcc gcaccagccg gtcgatgtag ccgaggatgc ggtagcgccc gccggcgtcg
     3241 agcgcgaact cgaccttgat ctccagcccg accgtctccc cgtcgtcgaa cggagcgtgc
     3301 cggtggtaga agttgcgcag gtacttctcg ccctcgacgc gatagtcctc ggcgacccag
     3361 ccgcggcgga cgatcgtcac cttgtcgctg tgctcggccg cccagcgtcc gcggtagaac
     3421 gcgaggacgg cgtcgagccg ggggggcttc ccctcctcct tcttccggta cagatgctcg
     3481 agcgcctcgt ggacgcggtt tccgaggaag gcttcgatgc cgaatgtgtc ccggcggatc
     3541 cgatcgacgt agcggtagcg gtactgcagc gggcacttct cgaaggcgct caggcgggag
     3601 tgggaccaga ccggcaaggg agagtggcct cctgctttcc cgagacggcg cgagtctagc
     3661 acgccttcgc ccgctgcgcg cctcctctgc tag
//
LOCUS       DASRIQ010000351         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_921350, whole genome shotgun sequence.
ACCESSION   DASRIQ010000351 DASRIQ010000000
VERSION     DASRIQ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921350"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..484)
                     /locus_tag="VFD06_08390"
     CDS             complement(20..484)
                     /locus_tag="VFD06_08390"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HZN03594.1"
                     /translation="MNDHGEHSRPNEPVRVIDRRTFTPAGEPRQPDSSAEEPASTLRV
                     DPAAAVPPPAPAAAGPTGPAADPVVSAHFQSLVVNLARQAAANLGASRHPLSGQIEID
                     LEGAQQMIDLLQAMRAKTAGNLTADEQELLEGLIGDLQMQYVAVRSKAGGRP"
     gene            604..1461
                     /gene="mazG"
                     /locus_tag="VFD06_08395"
     CDS             604..1461
                     /gene="mazG"
                     /locus_tag="VFD06_08395"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZN03595.1"
                     /translation="MSEAVRLRDIMDRLRGPSGCPWDREQTPETLRTFLIEEAYEVID
                     AIDRGRPGDLKEELGDLLFQIVFLSRVAEERGWFDLDAVMRGIGDKIVRRHPHVFGAD
                     RLDTSAEVLRQWEEIKSDERRGRGEDSMFAGVPQGLPALLKALRISAKAARVGFDWPD
                     GASLWSKVEEEMKELDEARRRGAPDQVAEEIGDLLFTLANVARQEDLDPEQILQEANR
                     KFLERFRYVEEGLRRDGLAPAPEHRARMEELWTEAKTRLRRTSPDRTAPSGGTSGSAP
                     RPDPTPGAR"
     gene            complement(1373..>2093)
                     /locus_tag="VFD06_08400"
     CDS             complement(1373..>2093)
                     /locus_tag="VFD06_08400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZN03596.1"
                     /translation="REVHGDRKYADDPAIVAGFALYKGLPVIVVGHQKGRDTKEKVRR
                     NFGMPRPEGYRKAVRVMELAEKFDRPIFTFIDTPGAYPGRGAEERGQAEAIAHSLRVM
                     ARLRVPIVVTVTGEGGSGGALAIAVGDRVLMLEHSVYSVISPEGCAAILWSDQGKARD
                     AARAMRVTAPDLRELEIIDDIVPEPPGGAQADPGRQAQILDEYLSGALAELTARPELD
                     RVEARYQKFRRMGRFGQAKFF"
BASE COUNT          299 a          742 c          757 g          295 t
ORIGIN      
        1 gcccgccgcc acgtccgcct caggggcggc cgccggcctt ggatcggacc gccacatact
       61 gcatctgaag gtccccgatg agaccttcga gcagctcctg ttcgtccgcc gtgaggttcc
      121 ctgccgtctt ggcgcgcatc gcctgcagga gatcgatcat ctgctgcgcc ccctccagat
      181 cgatctcgat ctggccgctc aacgggtgcc gcgacgcgcc gaggttggcg gccgcctgcc
      241 gcgccaggtt gacgaccagg ctctggaaat gggccgatac gaccgggtcg gcggccgggc
      301 cggtcggtcc ggccgcggcg ggcgcgggcg gcggaacggc cgccgcggga tcgacgcgca
      361 gggtcgaggc cggctcctct gccgaggaat ccggctgccg cggctcgccc gccggcgtga
      421 aggtgcgtcg atcgatgacg cgcaccggtt cgttgggtcg cgagtgctcg ccgtgatcgt
      481 tcatggggtg atgcttccgg caagaccgca ccccggaagc gcggcggatg gtagcatcag
      541 cgccgcggcg atgacaagtg acgcgcgcgc gaaacgcgcg tccccgcgcg cagcggaggc
      601 acgatgtcgg aggcggtgcg gttgcgggac atcatggatc ggctgcgcgg cccgtccggc
      661 tgcccgtggg atcgcgagca gactccagag acgctgcgca ccttcctgat cgaggaggcg
      721 tacgaggtga tcgacgccat cgatcgcggc cggcccggag atctgaaaga ggagctgggc
      781 gacctccttt tccagatcgt cttcctgagc cgcgtcgcgg aggagcgggg atggttcgac
      841 ctggacgcgg tgatgcgcgg catcggggac aagatcgtcc gacgccaccc gcacgtcttc
      901 ggcgcggacc gtctcgacac ctccgccgag gtgctgcgcc agtgggagga gatcaagagc
      961 gacgagcgga ggggccgcgg cgaggactcg atgttcgccg gcgtcccgca gggcctcccg
     1021 gcgctcctga aggcgctccg catctccgcc aaggcggcgc gcgtcggctt cgactggccg
     1081 gacggcgcgt ccctctggag caaggtcgag gaggagatga aggagctcga cgaggcgcgc
     1141 cggcgcggcg cgcccgacca ggtggcggag gagatcggcg acctcctctt cacgctcgcg
     1201 aacgtcgcac gccaggagga tctcgacccg gagcagatcc tgcaggaggc gaaccggaag
     1261 ttcctggagc gcttccgcta cgtcgaggag ggcctgcggc gggacggact ggcgccggcc
     1321 ccggagcacc gcgcgcgcat ggaggaactg tggaccgagg cgaagacccg cctcagaaga
     1381 acttcgcctg accgaaccgc cccatccggc ggaacttctg gtagcgcgcc tcgacccgat
     1441 ccaactccgg gcgcgcggtg agctcggcca gcgcgccgct caggtattcg tcgaggatct
     1501 gcgcctgccg cccgggatcg gcctgcgcgc cgccgggcgg ctccgggacg atgtcgtcga
     1561 tgatctccag ctcgcgcaga tcgggcgcgg tgacgcgcat ggcgcgggcg gcgtcgcgcg
     1621 ccttcccctg gtcggaccac aggatcgccg cgcacccctc cgggctgatc accgaataga
     1681 cggagtgctc gagcatcagc acccggtccc ccacggcgat cgccagcgcc ccgccgctcc
     1741 ccccctcgcc ggtcacggtg acgacgatcg gcacgcgcag ccgcgccatg acccgcaggc
     1801 tgtgggcgat cgcctccgcc tggccgcgct cctcggcgcc gcggccggga taggcgcccg
     1861 gcgtgtcgat gaaggtgaag atcggccggt cgaacttctc ggccagctcc atcacccgca
     1921 ccgccttgcg gtagccctcg gggcgcggca tgccgaagtt gcgccgcacc ttctccttgg
     1981 tgtcgcgccc cttctggtgg ccgacgacga tcaccggcag ccccttgtac agcgcgaagc
     2041 cggcgacgat cgccggatcg tcggcgtact tgcggtcgcc gtgcacctcg cgg
//
LOCUS       DASRIQ010000352         7319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_671232, whole genome shotgun sequence.
ACCESSION   DASRIQ010000352 DASRIQ010000000
VERSION     DASRIQ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671232"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="VFD06_08405"
     CDS             complement(<1..1636)
                     /locus_tag="VFD06_08405"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HZN03597.1"
                     /translation="MRRLLAAVGLFVLGATAARADWVASGACAYVDREFDAGGFTGSE
                     PIKPARFVDVQVVDGNQIRGQGVANVDGAFNFVVSDNQTRNIYIRCLARRTTSTGIPV
                     EVRSATSSGTTWSVRGPTINNHLPTQPVNTGTLVAVPGAGGEAFNLYDNVFHGTQYLN
                     VLRGGEAPAPLMLVVFNSTNSTVSSWDGTEIMAARNAGYDDTVVLHEMGHYVTAKFSA
                     SHSPGGAHSLSNCNQDMRLAWEEGQATNFGCSVRRWAALPNSQLYVRTTGQTGPGNLQ
                     FSFDVETQAPYSCRGATSETTVHTVLWDLGDGPSTTDSSPGTDEPWDLLQGLDQDRFE
                     VQDVYLPTAVNISVEDFWDGWFVASIANGHLPEMRTIFRQLGVEYEPDVMEPNDSVAE
                     ARLVTPGPAVYHLTYFADVNNDLVGEADPDLFGFDAVGGSPYTLETFNLVGDANTTLT
                     LLASDGLAVLASNDDRGPTDKSSLVSYTPPSSTRLYVRSAHGPGFGLYGSYDLKILGA
                     AAGTDVDLDGYPTTNDCNDANPSIYPGATEVCNGIDD"
     gene            complement(1633..2178)
                     /locus_tag="VFD06_08410"
     CDS             complement(1633..2178)
                     /locus_tag="VFD06_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03598.1"
                     /translation="MARFTENRPALPAPATLLVLAAALFAALPSAPAVPERAAPPAST
                     VEPTLPLRIDLDIESIERSGGRARGLLRIDLVAIDEVRDVEIDVLHDDALSIPGEATL
                     RLERLRLRRGERRDFRMEIEANADRDLPLRLEATFRTADGTLLRLGQGITLERSKTFG
                     EGRLHLGALEYPALVLDGPQP"
     gene            2291..3439
                     /locus_tag="VFD06_08415"
     CDS             2291..3439
                     /locus_tag="VFD06_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZN03599.1"
                     /translation="MKAPLSLDRATTTPLDPRVREAMLPWISGDVSIPAAFGARGRAA
                     RRALDAARAAVARLFDAPGEEILFTASATEANNLAIKGIAMATPRPGRLIAAATEHHS
                     ILHPLRTLLRSGHRVDLLPVDRDGLVDPADLERALAGGAILVSIAHASSEIGTLQPLA
                     DLARVARRAGVPFHSDASLSLGLLPWRGTERPDLGTATAHLMHGPAGAAALRVRAGVR
                     LRPLVEGGVQEHGLRAGTEALAAIVGFGRAAELAIEEGGTRGVAAEAAAQAFRERVRD
                     LLPEAVPTGHPGRRVPGHVSLCLPGVEAEAVLMGLEEEGIEAGSGSACTTEAGKPSHV
                     LLALGIEPLLARGAITVGFGPGHDRDDARRAAEALARVVTRLGSLSPL"
     gene            complement(3471..4748)
                     /locus_tag="VFD06_08420"
     CDS             complement(3471..4748)
                     /locus_tag="VFD06_08420"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HZN03600.1"
                     /translation="MSTIAPFRALRFAVAAPGELAQRLAPPYDVISPERRRILAADPH
                     NIVHLDLPEGPAERIYADAAARLEAWRRDGTLALDAAPAYHLAEQSFKDASGTARRRR
                     GFFARLRVEPFEAGSVLPHERTLESPRRDRERLLAATRTHLSAVFLLHEDPGGTAARL
                     LASASETAPERELVDPDGCGLRLWRLADRGAIDRLTAAAGRGWTLIADGHHRYESALA
                     YRQERRAAGRDDAADVLAFLCSAEDPGLVIGPIHRLVRGVPRLDPERVASALTGRFAV
                     DRRPREGLAAALREAAASPGVFGLLFPGRSEALLLRWRDDEEGRRALQGLPEPLRSLD
                     VVLLHRLVFEEAFGLSPQAQAGGDHLEYVKDEAGLLRGVEQAVCGVMLNPTRLAQVVE
                     VSKSGLRLPPKSTYFQPKVPSGIVLDPLDAPAD"
     gene            complement(4760..5365)
                     /locus_tag="VFD06_08425"
     CDS             complement(4760..5365)
                     /locus_tag="VFD06_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03601.1"
                     /translation="MRRLLAGACGLAIAFASATVLRSQEPAAPTQAPPSEGEPTIPDA
                     PPAADAAPEIHPDAVSSYQVRAYKSSRKKLWKGLLQVLQESGYPPEEIDPKAKRVKTS
                     FVDFSSKDYSEEVADPAPRLGGNYHILQMSKVKMGKVSLEGVVTDGEHGAVVLSLRAR
                     ILVDGLDQRKRVRMLTDRRSSGVIEGDFLRRLEDTLKLERR"
     gene            complement(5470..>7319)
                     /locus_tag="VFD06_08430"
     CDS             complement(5470..>7319)
                     /locus_tag="VFD06_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN03602.1"
                     /translation="DLRLDLNKYPEVRRAIETRRPVVIHDVANEELLDGVRDHIARLG
                     FESILVLPLLHGNELLGMLFLRAARTERRFSAQEITSCQVVANASANALRNAMLYDEA
                     RREARVRRETAHQLQNVLDHYPDLIITTDIEGRITEFSRGGEALVGLRREAALGLRLE
                     DVFPEPEARARVGRLLRDGEPMRNFETTARHQDGSLRDVLAAATILRGETGPQGSVVI
                     VQNVSELKAAREHLVQAEKLTALGEVVSGVAHELNNPLAGVLGYAQLLMRGPMEPRQQ
                     RSVERILDAALRCQKIVQNLLAFSRRSPSEKRWLGLNGLVEKALDLKDYDLRSAAIEV
                     VRDLEPELPKTMLDFNQVQQVLLNLIQNAQYAMHRHRGRGRLTLRTRHRGSVVELRVE
                     DDGPGIAREHLARIFDPFFTTKPVGEGTGLGLSISYGIVRDHGGRIWAESEPGRGTTL
                     VLELPVRHEGGLSEAAGEGAATPGLERLRRPLNLLVVDDEPVILDLLVDALAGTRHSV
                     DTAGGGAEALRKLEQRAYDVVLLDLKMPGVDGRQVYETIRARWPDRARRVVFASGDTV
                     HPDTRSFIARSGRACVDKPFRLESLASVLVAIAGEEDREELPATGTGPA"
BASE COUNT         1021 a         2580 c         2664 g         1054 t
ORIGIN      
        1 cgtcgtcgat gccgttgcag acctccgtcg cgcccgggta gatcgacgga ttggcgtcgt
       61 tgcagtcgtt ggtcgtgggg tagccatcca ggtcgacgtc cgtgccggcg gcggccccca
      121 gaatcttgag gtcgtaggag ccgtacagcc cgaaccccgg cccgtgcgcg gacctcacgt
      181 agagccgcgt gctcgacggc ggcgtgtacg acacgagcga ggacttgtcg gtgggtcccc
      241 ggtcgtcgtt cgacgcgagc accgccagtc cgtcgctggc gaggagcgtc agggtggtgt
      301 tggcgtcccc gacgaggttg aacgtctcca gcgtgtaggg ggacccgccc accgcgtcga
      361 agccgaagag atccggatcc gcctcgccga cgagatcgtt gttgacgtcg gcgaagtagg
      421 tcaggtgata gacggccggc cccggcgtca ccaggcgcgc ctccgcgacc gagtcgttcg
      481 gctccatcac gtccggctcg tattcgaccc cgagctgccg gaagatggtg cgcatctccg
      541 gcaggtggcc gttggcgatc gacgcgacga accacccgtc ccagaagtcc tcgacggaga
      601 tgttcaccgc ggtcggcaga tagacgtcct ggacctcgaa ccggtcctgg tcgagtcctt
      661 gcagcaggtc ccacggctcg tcggtcccgg ggctgctgtc ggtggtcgac ggcccgtccc
      721 ccaggtccca caggaccgta tggaccgtcg tctcgctcgt cgccccgcgg cacgagtacg
      781 gcgcctgcgt ctccacgtcg aaggagaact ggaggttccc cggaccggtc tgccccgtgg
      841 tccggacgta gagctggctg ttcggcaggg cggcccagcg ccgcaccgag catccgaagt
      901 tcgtcgcctg gccttcctcc catgcgaggc gcatgtcctg gttgcagttg ctgagcgagt
      961 gggcgccgcc ggggctgtgc gaggccgaga acttggcggt gacgtagtgg cccatctcgt
     1021 gaaggacgac cgtgtcgtcg tagcctgcgt tgcgcgccgc catgatctcg gtgccgtccc
     1081 acgacgaaac ggtcgaattg gtggagttga agacgacgag catcagcggg gcgggggctt
     1141 cgccgccgcg cagaacgttc aggtattgcg tcccgtgaaa gacgttgtcg tacaggttga
     1201 acgcctcgcc gccggctccc gggacggcga cgagggtgcc ggtgttgacc ggctgcgtcg
     1261 gcagatggtt gttgatcgtc gggccacgca cggaccaggt ggtcccgctg gaggtggccg
     1321 accggacctc caccgggatg ccggtgctcg tcgtccgccg ggccaggcag cggatgtaga
     1381 tgttgcgcgt ctggttgtcg gagacgacga aattgaaggc gccgtcgacg ttggcgaccc
     1441 cctgcccccg gatctggttg ccatcgacca cctgcacgtc cacgaagcgc gccggcttga
     1501 tgggctccga cccggtgaac ccgccggcgt cgaattcccg gtccacgtag gcgcaggcgc
     1561 cgctggcgac ccagtcggcg cgcgccgcgg tcgcgcccag gacgaacagc ccgaccgctg
     1621 cgagcaggcg cctcatggct ggggaccgtc gagaacgagg gccgggtact cgagcgcgcc
     1681 gaggtggagg cgtccttccc cgaacgtctt cgatcgctcc agggtgatcc cctgtcccag
     1741 acgcaggagg gtcccgtcgg cggtgcggaa cgtcgcctcg aggcgcagcg gcaggtcgcg
     1801 atcggcgttc gcctcgatct ccatccggaa gtcccgccgc tccccgcggc ggagccgcag
     1861 acgctccagc cggagggtcg cctcgccggg gatcgacagg gcgtcgtcat ggagcacgtc
     1921 gatctcgacg tcgcggacct cgtcgatcgc cacgaggtcg atgcggagaa gtcctcgagc
     1981 ccgcccgccg gaccgctcga tcgattcgat gtcgaggtcg atgcggaggg gcagggtcgg
     2041 ttcgacggtg gatgccggag gggcggcccg ctccgggacc gccggcgcgg acggcaaggc
     2101 ggcaaaaagg gccgccgcca ggacgagcag cgtcgcgggc gccgggagcg ccggccggtt
     2161 ctccgtgaag cgggccatta ttatttcgga atcggcccgt cgacgcgggc tcggggttta
     2221 attaccgcga cgcgcccccg gagtcaagac gtttccccgg accgacccgg cacatcatat
     2281 ccttgatgcc atgaaggctc ccctgtcgct cgatcgcgcc accacgacgc cgctcgaccc
     2341 gcgcgtccgg gaggcgatgc tgccgtggat ttccggcgat gtgtcgatcc ccgccgcctt
     2401 cggggcccgg ggccgggccg cgcgccgcgc cctcgacgcg gcgcgggccg cggtggcccg
     2461 actgttcgac gcgccgggcg aggagatcct cttcaccgcc tcggccaccg aggcgaacaa
     2521 cctggcgatc aaggggatcg cgatggcgac cccgcgaccc ggccgcctga tcgcggcggc
     2581 gaccgagcac cactcgatcc ttcacccgct gcggacgctc ctgcgatcgg ggcatcgcgt
     2641 cgacctcctg cctgtggacc gggacgggct ggtcgatccc gcggacctcg agcgcgctct
     2701 cgcaggaggg gcgatcctcg tcagcatcgc gcacgcctcc tccgagatcg gaacgctgca
     2761 gccgctcgcc gatctggcgc gcgtcgcgcg ccgcgccggc gtcccgtttc acagcgacgc
     2821 gagcctgagc ctgggcctgc tcccgtggcg cggcacggag cgtcccgatc tcgggaccgc
     2881 cacggcgcac ctgatgcacg ggccggccgg ggcggcggcg ctgcgcgtcc gcgcaggtgt
     2941 gcggctccgc ccgctggtcg aagggggcgt ccaggaacac ggtctgcgcg cggggaccga
     3001 ggcgctcgcg gcgatcgtcg ggttcggccg cgccgcggag ctggcgatcg aggagggcgg
     3061 gacgcgcggc gtcgcggcgg aggccgcggc gcaggcgttt cgcgagcgcg tccgggacct
     3121 tctcccggag gcggtgccca ccggacatcc cgggcgccgg gtccccgggc acgtcagcct
     3181 gtgcctgccg ggggtcgagg cggaggcggt gctcatgggg ctcgaggagg aggggatcga
     3241 ggcggggagc ggctccgcct gcaccaccga ggccggcaag ccgtcgcacg tcctgctggc
     3301 gctcggcatc gagccgctcc tggcgcgcgg cgccatcacg gtgggattcg gccccgggca
     3361 cgatcgcgac gacgcccggc gcgccgccga ggcgctcgcc cgcgtcgtga cgagactcgg
     3421 aagcctgagc ccgctctagg agcctgtccg aggcagaaga cgcccgccgt tcaatcggcg
     3481 ggcgcgtcga gcggatcgag cacgatgccg ctgggcacct tgggctggaa gtaagtggac
     3541 ttcgggggga ggcgcagccc gctcttgctc acctcgacca cctgggcgag ccgggtcggg
     3601 ttgagcatca cgccgcagac tgcctgctcg acgccccgca ggagccccgc ctcgtccttc
     3661 acgtattcga ggtggtcccc gccggcctgc gcctgcgggc tcaggccgaa ggcctcctcg
     3721 aacacgagac ggtggaggag gaccacgtcg aggcttctca atggctccgg aaggccctga
     3781 agcgcccgac gtccctcctc gtcatcgcgc cagcgcagga gcagcgcctc ggagcggccg
     3841 gggaacaaca ggccgaagac ccccggggag gcggccgcct cgcgcagcgc tgccgccagt
     3901 ccttcgcgtg gacggcgatc gaccgcaaag cggccggtga gcgccgacgc gacccgctcc
     3961 ggatcgaggc gcgggacgcc gcgcaccagc cggtggatcg ggccgatgac gagcccggga
     4021 tcctccgcgc tgcagaggaa ggccagcacg tccgcggcgt cgtcgcgccc ggccgcacgc
     4081 cgctcctggc ggtaggcgag ggccgattcg tagcgatggt gcccgtcggc gatgagggtc
     4141 catccgcgac cggccgcggc ggtcaggcga tcgatcgccc cccgatccgc cagccgccag
     4201 agcctcagcc cacagccgtc gggatcgacg agctcgcgct ccggcgcggt ctccgacgcg
     4261 gaggcgagca ggcgcgccgc cgtcccgccc ggatcctcgt gcagcaggaa gacggcactg
     4321 agatgggtgc gcgtcgcggc caggagccgc tcgcgatccc gccgcggcga ttcgagcgtg
     4381 cgctcgtgcg gcagcaccga gcccgcttcg aacggctcga cgcgcaggcg cgcgaagaag
     4441 ccgcgcctcc gacgcgcggt gccggaagcg tccttgaagc tctgttccgc caggtgatag
     4501 gcgggggcgg catcgagggc cagcgtgccg tcgcgccgcc acgcctcgag ccgggcggcc
     4561 gcatccgcgt agatccgctc ggccggccct tccggaagat cgaggtggac gatgttgtgc
     4621 ggatcggccg cgaggatccg ccgtctctcc ggggagatga cgtcgtacgg gggcgcaagg
     4681 cgctgcgcca gctcccccgg tgcggcgacc gcgaaccgga gcgcgcgaaa gggggcgatc
     4741 gtcgacatga tgtccgcgct taccggcgct cgagcttcag ggtgtcctcg aggcggcgca
     4801 ggaaatcgcc ctcgatcacc ccggaggatc ggcggtcggt gagcatccgg acgcgcttgc
     4861 gctgatcgag accgtccacg aggatccgcg cccgcaggct caggaccacg gccccgtgct
     4921 cgccgtcggt cacgacccct tcgagcgaga ccttccccat cttcaccttg ctcatctgca
     4981 ggatgtggta gttgccgccg aggcgcggcg cggggtcggc gacttcctcg ctgtagtcct
     5041 tcgagctgaa atcgacgaag gacgtcttga cccgcttcgc cttcgggtcg atctcttccg
     5101 gaggataccc ggactcctgg aggacctgga ggagtccttt ccacagcttc ttgcggctcg
     5161 acttgtaggc gcgcacctgg taggaggaga cggcgtcggg gtggatctcc ggcgccgcgt
     5221 cggcggccgg cggggcgtcg gggatcgtgg gctccccttc cgaaggggga gcctgcgtcg
     5281 gcgcggccgg ctcctgggag cgaaggaccg tggcggaggc gaaggcgatc gccaggccgc
     5341 aggcgccggc caggagacgg cgcacggcac tcgtgggacg aaccgggttc aaacttttgg
     5401 aggagacagg gatcatcggg gacgccgcgc cgccgttcgg gcgcggcgat gatagcacgc
     5461 agtccgacgt caggccgggc cggttccggt cgccggcagc tcctcgcgat cctcctcgcc
     5521 ggcgatcgcg acgagcaccg aagcgagtga ttcgaggcgg aacggcttgt cgacgcacgc
     5581 gcgccccgat cgtgcgatga aggagcgcgt gtccgggtgg acggtgtcac cggaggcgaa
     5641 gacgacgcgg cgcgcccgat cgggccagcg cgcccggatc gtctcgtaca cctgccggcc
     5701 gtcgacgccg ggcatcttga gatcgagcag gacgacgtcg tacgcgcgct gctccagctt
     5761 ccgcagcgcc tccgcgccgc cccccgcggt gtccacggag tggcgcgtcc cggcgagcgc
     5821 gtccacgagc aggtcgagga tcaccggctc gtcgtccacg acgagcaggt tcaacggccg
     5881 gcgcaggcgc tcgaggccgg gcgtcgcggc cccctcgccg gccgcctcgg agagcccccc
     5941 ctcgtgccgg accggcagct ccaggacgag cgtcgtgccg cgacccggct ccgactcggc
     6001 ccagatgcgg ccgccgtggt cccgcacgat gccgtaggag atcgacagcc ccagcccggt
     6061 tccctccccc accggcttgg tggtgaagaa gggatcgaag atgcgggcca ggtgctcgcg
     6121 cgcgatcccc ggcccgtcgt cctccacccg gagctcgacg acgctgccac ggtggcgcgt
     6181 gcgcagcgtc aggcggccgc ggccgcggtg ccggtgcatg gcgtactggg cgttctggat
     6241 caggttcagc agcacctgct gcacctggtt gaagtcgagc atggtcttgg gcagctccgg
     6301 ctcgaggtcg cgcaccacct cgatcgcggc ggacctgagg tcgtagtcct tgagatcgag
     6361 cgccttctcc accagcccgt tgagcccgag ccagcgcttc tccgacggag agcggcgcga
     6421 gaacgcgagg aggttctgaa cgatcttctg gcagcgcagc gcggcgtcga ggatccgctc
     6481 gaccgagcgc tgctgccgcg gctccatcgg cccgcgcatc aggagctgcg cgtatccgag
     6541 gacgccggcg agcgggttgt tcagctcgtg cgcgaccccc gagaccacct ccccgagcgc
     6601 agtcagcttc tccgcctgca ccaggtgctc gcgcgccgcc ttgagctcgg agacgttctg
     6661 cacgatgacc accgaccctt gcggtcccgt ctcgccgcgc aggatcgtcg ccgcggcgag
     6721 cacgtcccgg agacttccgt cctggtgacg cgcggtcgtc tcgaagttgc gcatcggctc
     6781 cccgtcccgc agcaggcgac cgacgcgggc gcgcgcctcc ggctcgggga agacgtcctc
     6841 gaggcgcagg cccaaggccg cctcccggcg cagccccacg agcgcctcgc cgccgcggct
     6901 gaactcggtg atgcgtccct cgatgtcggt cgtgatgatc aggtccgggt agtgatcgag
     6961 gacgttctgc agctggtggg cggtctcgcg gcgcacccgc gcctcccggc gcgcctcgtc
     7021 gtacagcatg gcgttgcgca gcgcgttcgc cgaggcgttg gccaccacct ggcacgaggt
     7081 gatctcctgg gccgagaagc gccgctccgt ccgcgccgcc cgcaggaaca gcatccccag
     7141 aagctcgttg ccgtgcagca gcggcagcac caggatcgac tcgaagccga gccgcgcgat
     7201 gtggtcccgg acaccgtcga gaagctcctc gttggcgacg tcgtgaatga ccaccggacg
     7261 ccgggtctcg atggcgcgcc gcacctccgg gtacttgttc aggtccaggc gcaggtcgg
//
LOCUS       DASRIQ010000353         9298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3006792, whole genome shotgun sequence.
ACCESSION   DASRIQ010000353 DASRIQ010000000
VERSION     DASRIQ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3006792"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..270)
                     /locus_tag="VFD06_08435"
     CDS             complement(70..270)
                     /locus_tag="VFD06_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03603.1"
                     /translation="MPTVLRAAGLLVLAPVLAAADPPAVPDTAASPPTPADLAGIWAG
                     TLMHAGRRSASRSTSTRVTTGR"
     gene            complement(384..1070)
                     /locus_tag="VFD06_08440"
     CDS             complement(384..1070)
                     /locus_tag="VFD06_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03604.1"
                     /translation="MNRFRVPAGVLMVAAIHGLFAAPPAAPEELSLPARLRAEMTIVN
                     AEVDPRTGATRKAVGDMLGVTKPTGPTTPWVQFPTTSAIVSITIRRWSLEDEQEALRA
                     AIESGGLAAVIKTMKKLPTLGDVHVNAGRLSIRAAATWMTEHTQRIRLVVSGRLVTTN
                     PDPFAQSSRVLDILDLSLPHGERYGTGTLVTATQVEFEKPGLISPVVFAIDSATQPLS
                     RVERQPPEAR"
     gene            complement(1133..4009)
                     /locus_tag="VFD06_08445"
     CDS             complement(1133..4009)
                     /locus_tag="VFD06_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN03605.1"
                     /translation="MARTLEPDDRIARYRIVGPLGAGGMGEVYIARDETLERSVALKI
                     LPPNLVRNDERVRRFVTEAKSASSLNHPNIVTIHEIGQDRVIPGRPGAEPGEPLHYMA
                     MELVAGDTLDQKIHHEKTDLRTLLGWIAQAAEGIAKAHAAGIVHRDLKPGNIMVSKDG
                     FAKVLDFGLAKLTERAGVSDAERTSAPTQAAATGAGVVMGTVGYMSPEQVQARTVDHR
                     SDIFSLGCIVYEAATRRRPFVADSDIEVMHLILKEKPPPVEELNPEVPGEVRRLIRRC
                     LAKSPEQRFQSMKDLAIDLRELTEEWESLSTSTPSGTSAVRGSGAAASAGKRALNPAM
                     IAALLIGVAGAGYGLYSYLHRAAPAAGAAATALQDLRMAVLLSRDDLTESALSADGRY
                     LAFVTFQDRATSLKVRQVRTGSDVVIVPESAQPIRGIAFSRDGDYLYYLNRDADSPNY
                     QALYLVPSLGGTPRKIVFDVDSAPTFSPDGKRACFRRGLIDQGADSLVVVDLDTLQER
                     TLVRIQRPEEFAAAPAWSPDGATIAAAVQSGAAGVKSRIEAIDAASGRRTTVGGGPWL
                     LISSLGWMPDGKGILASALDPASGGAHQVYRVGYPGGETLRLTSDLDGYTNLSIAGDG
                     SAAAIRRVTVDNLWVARPGTKQEPQAMTFASGSANSILQLQPLPGGAVAFAAPQNNRV
                     FLWRIDADGSNRRQLTSQGVYVINVLYARGAGLVFSQVDEGGNLLAHIWRMDPDGGGL
                     TRLTDGNGEQLGSLSPAGDWFAYSLWDDPRRSSLIRKVAGGDPRRIEGTEGDPPLFSY
                     DGRRLLYTTLTERQKRFFPQRHIMDVESGREEATFLLPPGAQDELWAPDGRSLTYIDR
                     DRGWNLMRKPLPDGAPVALTSFTDGQIFDHGWSPDGSRMVLHRRAAGRDSLWMLEPGD
                     AKPALISEFKTGRPSRHFWAPDAPVLYFTYGHSTQDVVMIRGVK"
     gene            4126..4503
                     /locus_tag="VFD06_08450"
     CDS             4126..4503
                     /locus_tag="VFD06_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN03606.1"
                     /translation="MPVSVRARGDVAIIELNGEFTFGGGGLAQPLDLRGHHLSDLGQT
                     LGRLLDQGVRKIVLDLEGVSFFDSAGLGELIACKKRTVQSGGDIRLLRPTGKVRDMLA
                     MLSLTRIFQVFDDEAAVLASFDR"
     gene            complement(4530..5873)
                     /locus_tag="VFD06_08455"
     CDS             complement(4530..5873)
                     /locus_tag="VFD06_08455"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03607.1"
                     /translation="MALQALRWGVALLLALSSLALAWRPIPLLLSVPACVLLAGVFLF
                     LRGRVPAAARKRRRIGAPEIALVAASVLVTLALVEVAARLLYDFRPYADTPYVRHPKF
                     FFVPRPHGSGTFDVNLGSGRFLHVPFTFSSQGLRDREYGKKAPGEFRILLLGDSFAMG
                     WGLPPGQTIAAQLEQILRSRDGNGRITVINGGVSGYGPFQEEGFLEDRGLPLEPDLVL
                     LQLFPANDIENSLAQVGRRMRAYDFGWQRHLLEFANQNRWPVQVERWLYNHSRAINAW
                     LGATRRHGAISTFLDHLRFLPSAGLVVPGPSEPRPWWIEVTLRDWYPELQEGFDLMQR
                     DVLAIRRLCEERRIGFFAFTLAGGQDVIDEYWADALRQGPPGTLYERGKETRIMREFY
                     DASGIRAIDVMEALRRDPRPEELYYRYDGHLQPHGAEVVARAIAEYLDANGLRGR"
     gene            complement(5928..6551)
                     /locus_tag="VFD06_08460"
     CDS             complement(5928..6551)
                     /locus_tag="VFD06_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03608.1"
                     /translation="MNHRRSVALALVLGVVAGMGHAGSAAAPEGPGEAAAPARKVLVV
                     AKIREETKRRNLEEEARIELQKRGVETILGSDVMVESDFASEDTIRKKVESLGVDGVL
                     GFVVLGIDEKVKTSSATMSVGVGVGGYGHSGGLGLFVGGSVPLGGGSKVVRKVHLRAR
                     YFGRPFEGPAWEKVYTEDLPDDTTRLAQYLAYDSVKTLKKKKLIPAK"
     gene            6937..7482
                     /locus_tag="VFD06_08465"
     CDS             6937..7482
                     /locus_tag="VFD06_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03609.1"
                     /translation="MARRHRFTNALTFAVAILLCLGSAAGSAEQNDKQQMRGLDEQIQ
                     EIKSDVLRMAAELSQLEEKLLYPSGTQVAVFVALAKGDPMRLDSVKLQIDGKPVAHYI
                     YSAKELEALRKGGVQRLYVGNVATGDHQLEVVVEGKREGGADFSRTERFAVRKDVKPK
                     LVGLTLGGSGAGNPPIALGDW"
     gene            7487..>9298
                     /locus_tag="VFD06_08470"
     CDS             7487..>9298
                     /locus_tag="VFD06_08470"
                     /inference="COORDINATES: protein motif:HMM:NF045090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03610.1"
                     /translation="MRRAPLRLLGLGLALGLAIRPSAGVDRSAGPPPLRDLFFGEALY
                     HAQQGQYFEALERLDTELAQYHGLDEPRLDTLHYHINDAEFSVGDFELDYRMHHRAGR
                     AIKAVLEGAVDESVRNEAAFRLARIHFQKGQLDDAVNALARIQGTVPEKVRDEVEFLR
                     ANIDMATGRPADAVKVLKQIRGDESLAGFAAYNLGIALLQDGREKEGVEQLDKAGVLQ
                     PGNPADLAIRDKANLVLGVILFEAADFDRARQSLDRVRLEGPFSNQALLRAGWAEATA
                     KNYDRALVPWNILADREPTDAAVQEAILAVPSAYASLNLHGRAALMYGRALEQFSQQI
                     GKVDASIGSIREGRFLEALTREESRQDETWVVRLRSLPGAPETFYLTELMASHEFQTA
                     LHNYLDLDDLRAKLLSWQTSLDAYDDLLRLRGRNYEPLLPEVDAKFRVLDSRMRLRLE
                     QRRSLAERLQAILTAPQPESLATAEERIASDALTRIEKQIGASRGAEAVALQQRADRL
                     RGVLTWKLVTEYHARLTAAHEHLNELNTHVDALTRQYDAFVRTRQAATHSYVGYGEKI
                     GQLRTHVAEGLQRVESLKAQQGRMIETVAVNQLQARRE"
BASE COUNT         1442 a         3216 c         3242 g         1398 t
ORIGIN      
        1 cgatgcggag ccgcgcgcgt cccagaggcg ccgccgcagc gtgcaccgcg gggaccgaca
       61 tcgagaggct caccgtcccg tcgtcacccg ggtcgaagtg gagcgcgaag cgctccgcct
      121 ccccgcgtgc atcagcgtcc ccgcccagat cccggccagg tcggcggggg tgggtggcga
      181 tgcggcggtg tccggaactg cgggcggatc cgcggccgca agcaccggcg cgaggacgag
      241 cagtccggcg gccctcagga cagtgggcat ggtcctcaac ctcgagcggg tctctccggg
      301 catgcgtcct ccgcgacggt gcagcccggg gaccacccgg tccgggcagc agcggtacgc
      361 aggatgtcgg cgtcgggttg ctctcagcgg gcttcgggcg gctgccgctc gacacgactc
      421 agcggctgcg tggcgctgtc gatggcgaag acgaccgggg agatcaggcc gggcttctcg
      481 aactcgacct gcgtcgcggt gacgagcgtg ccggtcccgt agcgctcccc gtgggggagc
      541 gagagatcca ggatgtcgag cacccggctg gactgcgcga acggatccgg attcgtcgtg
      601 acgagccggc cgctgacgac gagacggata cgctgcgtgt gctccgtcat ccaggtcgcg
      661 gcggcgcgaa tcgacaggcg cccggcgttg acgtgcacgt ccccgagggt cggcagtttc
      721 ttcatcgtct tgatcaccgc cgcaagacct cccgattcga tcgcagcgcg gagcgcctcc
      781 tgctcgtcct cgaggctcca gcgacgaatg gtgatcgaca cgatggcgga cgtggtcggg
      841 aactggaccc agggtgtggt cggccccgtt ggcttggtca ctcccagcat gtcgccgacc
      901 gccttccggg tggcgccggt gcgcgggtcc acctcggcgt tgacgatggt catctcggcc
      961 cggagccggg ccggcaggct cagctcctcc ggcgccgccg ggggggcggc gaacaggccg
     1021 tggatcgcgg cgaccatcag gacgccggcc gggacgcgaa agcgattcat cgatcgaacc
     1081 ccgaagagtc ttcgtgtccc ccgatgatac gcctctcggg cggcgggcga ggctacttca
     1141 ccccgcggat catcaccacg tcctgcgtcg agtgcccgta ggtgaagtag aggaccggcg
     1201 cgtccggcgc ccagaagtgc cgcgacggcc gtccggtctt gaactcggag atgagcgccg
     1261 gcttcgcgtc gccgggctcg agcatccaca ggctgtcgcg gccggcggcg cggcggtgca
     1321 gcaccatccg ggacccatca ggactccagc cgtgatcgaa gatctgcccg tcggtgaagg
     1381 aggtcagcgc caccggcgcg ccgtccggca gcggcttgcg catcaggttc cagccgcggt
     1441 cccggtcgat gtaggtgagg gatcggccgt ccggggccca tagctcgtcc tgggcgcccg
     1501 gcggcaggag gaacgtcgcc tcctcccggc cgctctccac gtccatgatg tgccgttgag
     1561 ggaagaaacg cttctgtctc tccgtcaggg tcgtataaag aagacggcgg ccatcgtagg
     1621 agaacagcgg cgggtccccc tccgttccct cgatccttct cgggtcgccg ccggccacct
     1681 tccggatcag gctcgaacgg cgcggatcgt cccacagcga gtaggcgaac cagtcgcccg
     1741 caggagagag cgacccgagc tgttcgccgt tgccgtcggt gagccgggtg agcccgccgc
     1801 cgtccggatc catgcgccag atgtgcgcga ggaggttccc cccctcgtcc acctgcgaga
     1861 agaccaggcc ggccccgcgg gcgtacagca cattgatgac gtacaccccc tgcgaggtga
     1921 gctgtctccg gttcgatccg tccgcgtcga tgcgccacag gaagaccctg ttgttctgcg
     1981 gcgcggcgaa ggcaacggcg ccgccgggga gcggctgcag ctgcaggatg gaattcgcgc
     2041 tgccggaggc gaaggtcatt gcctgcggct cctgtttcgt cccgggacgg gcgacccaga
     2101 ggttgtccac ggtgacccgg cggatggcgg ccgccgatcc gtccccggcg atcgacaggt
     2161 tggtgtagcc gtcgaggtcg ctggtcagcc ggagcgtctc cccgcccggg tagccgacgc
     2221 ggtacacctg atgggcgccg ccgctcgctg ggtccagcgc gctcgcgagg atccccttgc
     2281 cgtcgggcat ccagccgagg ctgctgatga ggagccacgg accgccgccg accgtggtcc
     2341 gccgcccgga ggcggcgtcg atcgcctcga tccgcgactt gaccccggcg gcgccactct
     2401 gcaccgccgc ggcgatcgtc gcgccgtccg gagaccaggc gggcgcggcg gcgaactcct
     2461 cgggacgctg gatgcgcacc agggtgcgct cctgcagggt gtcgagatcg accaccacca
     2521 gggaatcggc cccctggtcg atgagccccc gccggaagca ggcccgcttc ccgtccggag
     2581 agaaggtcgg ggcggaatcg acatcgaaga cgatcttcct cggggtcccg ccgagggacg
     2641 gcaccaggta gagcgcctgg tagttgggcg agtcggcgtc ccggttcagg tagtagaggt
     2701 agtcgccgtc ccgcgagaag gcgatgccgc ggatcggctg cgcgctctcc gggacgatga
     2761 cgacgtcgct gccggtgcgc acctggcgga ccttgagaga cgtggcccga tcctggaagg
     2821 tgacgaaggc gagatagcgg ccgtcggccg acagggcgct ctcggtgaga tcgtcgcgac
     2881 tcagaaggac ggccatgcgc aggtcctgga gcgcggtggc cgccgctccg gccgccgggg
     2941 cggcgcggtg gagatacgaa tacaggccgt acccggcgcc ggcgacgccg atcaggaggg
     3001 cggcgatcat cgccgggttc agggcgcgtt tcccggcgga tgccgcggcg cccgatcccc
     3061 gcactgcgga cgtgccggaa ggggtcgagg tggagaggct ctcccactcc tccgtaagct
     3121 cgcgcaggtc gatcgccagg tccttcatcg actggaagcg ctgctcggga gatttggcga
     3181 ggcagcggcg gatgaggcgg cgcacctccc ccggaacctc cggattgagc tcttcgacgg
     3241 gagggggctt ctccttgagg atgaggtgca tcacctcgat gtcggagtcg gcgacgaacg
     3301 ggcgccggcg ggtcgccgcc tcgtacacga tgcagccgag cgagaagatg tcggagcggt
     3361 gatcgaccgt ccgcgcctgc acctgctcgg gggacatgta gccgacggtc cccatgacga
     3421 caccggcccc ggtcgccgcc gcctgtgtcg gagcgctggt ccgctcggcg tcgctgaccc
     3481 cggcgcgctc ggtcagcttc gcgaggccga agtcgagcac cttcgcgaag ccgtccttcg
     3541 acaccatgat gttccccggc ttgaggtcgc ggtgcacgat gccggcggcg tgcgccttgg
     3601 cgatcccctc cgccgcctgg gcgatccagc cgaggagcgt gcgcaggtcg gtcttttcgt
     3661 ggtggatctt ctggtcgagg gtatcccccg ccaccagctc catcgccatg taatggaggg
     3721 gctcccccgg ctcggcgccg ggccggccgg ggatcactcg atcctggccg atctcgtgga
     3781 tggtgacgat gttcgggtgg ttgagggacg acgccgactt cgcctcggtg acgaagcgcc
     3841 gcacccgttc gtcgttgcgg acgaggttcg gcggcaggat cttcagcgcg acgctgcgct
     3901 cgagcgtctc gtcccgggcg atgtacacct cccccatccc gccggcgccg agcggtccga
     3961 cgatgcggta gcgggcgatc cggtcatccg gttcgagcgt gcgtgccacg ttgttccgtc
     4021 ctcacgtctg cgagaggcgc agtctacccg aaccgtcgca tccggcccca ggccaagctc
     4081 cggccgttct gcgctagact cccggccggg aggtcggagc ggagcatgcc ggtttcggtc
     4141 cgcgcacgcg gcgacgtcgc catcatcgag ctgaatggcg agttcacctt cggcggcggg
     4201 gggctcgccc agccgctcga cctccgtggc caccacctct cggatctggg tcagacgctg
     4261 gggcgcctgc tcgaccaggg cgtccggaag atcgtgctcg atctcgaggg ggtcagcttc
     4321 ttcgacagcg ccggcctcgg tgagctcatc gcctgcaaga aacggacggt tcagagcggc
     4381 ggcgacatcc gcctgctgcg gccgaccggc aaggtgcgcg acatgctcgc gatgctctcc
     4441 ctgacgcgga tcttccaggt cttcgacgac gaagccgccg tcctcgcgtc gttcgaccgt
     4501 tagaccgcgg accctcccgg gcccgtcgat cacctgccgc gcaggccgtt cgcgtcgagg
     4561 tattcggcga tcgcccgtgc gaccacctcg gcgccgtgcg gctgcaagtg accgtcgtag
     4621 cggtagtaca gctcctcggg ccggggatca cggcgcagcg cctccatgac gtcgatggcg
     4681 cggatgccgc tcgcgtcgta gaactcccgc atgatgcggg tctccttgcc gcgttcgtag
     4741 agcgttccgg gcggcccctg ccgcagcgca tcggcccagt actcgtcgat cacgtcctgc
     4801 cccccggcga gagtgaaggc gaagaaacca atgcggcgct cctcgcagag cctgcggatc
     4861 gccaggacat cgcgctgcat gaggtcgaat ccttcctgca gctccgggta ccagtcgcgc
     4921 agcgtgacct cgatccacca cggccgtggc tcactcggtc ccgggacgac gaggccggcg
     4981 gacggcagga agcgcaggtg gtcgaggaag gtcgagatgg ctccgtggcg ccgcgttgcg
     5041 cccagccagg cgttgatcgc gcgggagtgg ttgtacagcc agcgctcgac ttgcaccggc
     5101 cagcggttct ggttcgcgaa ctcgagcagg tggcgctgcc agccgaaatc gtaggcgcgc
     5161 atgcgcctcc ccacctgcgc cagcgagttc tcgatgtcgt tggccgggaa aagctgcagg
     5221 aggaccaggt ccggctcgag cgggagcccc cgatcctcga ggaacccctc ctcctggaag
     5281 ggaccgtagc ccgagacgcc tccgttgatg accgtgattc gcccatttcc gtcgcggctt
     5341 ctcaggatct gttccagctg ggccgcgatc gtttgaccgg gtggaagccc ccagcccatg
     5401 gcgaacgagt cccccaggag caggatgcgg aactctccgg gggccttctt cccgtattcg
     5461 cgatcgcgca ggccctggga cgagaaggtg aaggggacgt ggaggaagcg gcctgagccg
     5521 aggttgacgt cgaaggtgcc cgatccgtgc ggccgcggga cgaagaagaa cttcgggtgc
     5581 cgcacgtacg gcgtatcggc gtaaggccgg aagtcgtaca ggagtcgcgc ggcgacctcg
     5641 acgagcgcca gcgtgacgag caccgaggcg gccacgaggg cgatctccgg cgcaccgatc
     5701 cgccggcgct tccgcgccgc ggcgggaacg cggccgcgca ggaacaggaa gaccccggcg
     5761 agcaggacgc aggcgggaac cgacaggagg agcgggatcg gacgccacgc cagcgccagg
     5821 ctcgagagcg ccagaagaag cgccacaccc catcgaaggg cctgcaacgc catcgactga
     5881 gtcgctccgc ccgccatcgt ggcgggcgca gtctatgcga agaccgatta cttggcgggg
     5941 atgagctttt tcttcttgag ggtcttgacc gagtcgtagg cgaggtactg cgccaggcgc
     6001 gtcgtgtcgt cggggagatc ctcggtgtac accttctccc acgccggccc ttcgaagggg
     6061 cgcccgaagt agcgggcgcg caggtggacc ttgcggacca ccttcgaacc gccgccgagc
     6121 gggacgctcc ctccgacgaa gaggccgagc ccgccggagt gcccgtaacc gccgacgccg
     6181 accccgacac tcatcgtcgc ggaggaggtc ttcaccttct cgtcgatccc gaggacgacg
     6241 aaccccagga ctccgtccac gcccaggctc tcgaccttct tccggatggt gtcctccgag
     6301 gcgaaatccg attccaccat gacgtccgag ccgaggatcg tctccacccc tctcttctgc
     6361 agctcgatcc gcgcctcctc ctcgaggttc cgccgcttgg tctcctccct gatcttggcg
     6421 accacgagaa ccttgcgcgc gggtgccgcc gcttcgccgg ggccttcggg cgcagccgcc
     6481 gaccccgcgt ggcccatgcc cgcgacgacc ccgaggacga gcgcgagcgc gactgatcgc
     6541 ctgtgattca tcgtccgtag cccgctgatg tgtgcaaggc ggatgactcc cggttttcgg
     6601 atgcccatca tgccatcgtt tcgagaaccg gatccccgga aacgattccg atcctccccg
     6661 cccgggcttc gacaggttcg ttgacggccg cgtccttcgc ccgacgggga gtgccggtgc
     6721 cccgaccctg cgatgacgaa atcgaatgac atcgatgacc ggattgtcac ggcgaccccc
     6781 ccgttttgga cacgaggccc gtctgtaggc cacttccgcg ggatcgcctg ctggcagggc
     6841 gcttgctctt gctcggacgc cgctgccggc agaggcgagt ggtcggcaag cgcgatttcg
     6901 attttggctc gtcgaagagt cgcagggggg gaacgcatgg cgcgacgcca ccggttcacg
     6961 aacgcactca ccttcgccgt ggccatcctt ctctgcctcg gcagcgccgc cggctcggcg
     7021 gaacaaaacg acaagcagca gatgcggggc ctggacgagc agatccagga gatcaagtcg
     7081 gacgtcctcc ggatggcggc ggagctgagc cagctggagg agaagctcct gtacccatcg
     7141 gggacgcagg tcgcggtctt cgtcgcgctg gccaagggcg atccgatgcg actcgactcc
     7201 gtgaagctcc agatcgacgg caagccggtc gcccactaca tctacagcgc caaggagctc
     7261 gaggccctgc gcaaaggcgg cgtgcagcga ctctacgtcg gcaacgtcgc gaccggcgat
     7321 caccagctcg aggtggtcgt cgagggcaag cgcgagggcg gagcggactt cagccgcacg
     7381 gagcgcttcg cggtccgcaa ggacgtgaag ccgaagctgg tggggctgac gctgggcggg
     7441 tccggcgccg gcaacccgcc gatcgccctc ggggactggt gatctgatgc gtcgggcgcc
     7501 cctccgcctg ctcggactcg gcctggcgct cggcctggcg attcgtccgt ccgcaggcgt
     7561 cgaccgttcc gccggtccgc cccccctgag ggacctcttc ttcggcgagg cgctctatca
     7621 cgcccaacag ggacagtact tcgaggcgct ggagcgcctc gacaccgagc tcgcccagta
     7681 ccacggtctc gacgagcccc gtctcgacac gctccactac cacatcaacg acgccgagtt
     7741 ctcggtcggc gacttcgagc tcgactaccg catgcaccac cgcgcgggcc gcgccatcaa
     7801 ggcggtgctc gaaggcgcgg tcgatgagtc cgtgcgcaac gaagcggcct tccggctcgc
     7861 ccgcatccat ttccagaagg gccagctgga cgacgccgtg aacgcgctgg cgcggatcca
     7921 gggaacggtg cccgagaagg tcagggacga ggtcgagttc ctccgggcga acatcgacat
     7981 ggcgacgggc cggccggcgg acgccgtcaa ggtgctcaag cagatccgcg gcgacgagag
     8041 cctggccggg ttcgccgcct acaacctcgg catcgcgctc cttcaggatg gccgggagaa
     8101 ggagggggtc gaacagctgg acaaggccgg ggtgctgcag cccggcaacc ccgccgatct
     8161 cgcgatccgc gacaaggcca atctggtgct gggcgtcatc ctgttcgagg cggccgactt
     8221 cgatcgggcc aggcagtccc tggaccgcgt ccgcctcgaa ggtccgttct cgaaccaggc
     8281 cctgctccgg gccggctggg cggaggcgac ggccaagaac tatgaccgcg ccctcgtccc
     8341 ctggaacatc ctcgcggatc gtgagcccac cgacgcggcg gtccaggaag ccatcctggc
     8401 cgtgccgtcc gcgtacgcca gtctcaacct gcacgggcgc gccgcgctca tgtacgggcg
     8461 cgcgctggag cagttcagcc agcagatcgg gaaggtagac gcgtcgatcg gcagcatccg
     8521 ggaagggcgt ttcctcgaag cgctgacccg cgaggagagc cggcaggacg agacctgggt
     8581 cgtccgcctg cggagcctgc ccggcgcgcc ggagaccttc tacctgacgg aattgatggc
     8641 ctcgcacgaa ttccagaccg cgctccacaa ctacctcgac ctcgatgatc tccgcgcgaa
     8701 gctcctgtcc tggcagacca gcctcgacgc ctacgacgac ctcctccggc tgcgcggacg
     8761 gaactacgag ccgctcctgc cggaggtcga cgcgaagttc cgggtgctgg actcgcggat
     8821 gcgcctgcgg ctcgagcagc gcaggagcct cgccgagcgc ctgcaggcga tcctgaccgc
     8881 gccgcagccg gagtccctcg cgaccgcgga ggagcgcatc gcctcggacg ccctcacgcg
     8941 catcgaaaag cagatcggcg cttcccgcgg ggccgaggcg gtcgcgctcc agcagcgcgc
     9001 ggaccggctg cgcggcgtgc tcacctggaa gctcgtcacc gagtaccacg cgcggctgac
     9061 tgcggcccac gagcacctca acgagctgaa cacccacgtc gacgccctca cccggcagta
     9121 cgacgccttc gtccggacga ggcaggcggc gacgcacagc tatgtcggct acggggaaaa
     9181 gatcggacag ctccgcacgc acgtggccga ggggctgcag cgcgtcgaga gcctgaaggc
     9241 gcagcaggga cgcatgatcg agaccgtcgc cgtcaaccag ctccaggcgc gccgcgag
//
LOCUS       DASRIQ010000354        10038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_671900, whole genome shotgun sequence.
ACCESSION   DASRIQ010000354 DASRIQ010000000
VERSION     DASRIQ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671900"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1366
                     /locus_tag="VFD06_08475"
     CDS             128..1366
                     /locus_tag="VFD06_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN03611.1"
                     /translation="MRIETFKMERMQSTYENQVECNLSESGVEPMRPEDLLEGDAERL
                     AFLQQKLGYCQSNGSEELRDQIALFYPGAKRDNVLVTNGGSEANYASFWSLLERGDRA
                     AVMLPNYLQTWGLSRAYAGKADPFRMIERPDGRGGRRWGLDIAGLKRAVTKRTKVILV
                     TNPNNPTGGVLDEEEMDAVVAVARASRAWLVVDEIYRGAELSGPTTPTFWGRYERTLI
                     TSGLSKAFGLPGLRIGWVVGPPAQIAKLWGYHDYTTIAPGMLSDRLARRAMTPSVREE
                     IFARTRGYVRRYWPIVDGFVKERSDWLSVIAPVAGAIALIRYDLPIASIPLVERLRTE
                     KSTLVVAGDQTGIPKHIRIGFGYGADPSVLHDGLGRIDALMRTLRTGSRKSAAGSRRA
                     AGSGVKRAAAGGARRAAAAR"
     gene            complement(1377..2837)
                     /locus_tag="VFD06_08480"
     CDS             complement(1377..2837)
                     /locus_tag="VFD06_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03612.1"
                     /translation="MKRALVLPDDRLAGLPPTVRKIAAAAQQAGGRAFLVGGFVRDTV
                     RSLNDGQTGGTGECEFDLEIYGLPPEQLALLLRRFGSVNLVGESFAVYKVTPRGPDAA
                     NEGAIEIDVSLPRRDQRTGRGHRGFAVEGDPQLSIEEAARRRDFTVNALLLDPLTGEV
                     LDPWGGLGDLENRVLRAVDPDTFAEDSLRVLRGMQFAARLGFSFDDATVSLCRSLPLD
                     DLPGERVWGEMEKLLMKAARPSIGLDWGLRLGAIQRLFPELYATVGCPQEKEWHPEGD
                     VWIHTLLAIDRARIEAAGLPYAKEATVMLAVLCHDFGKPSTTAVVEGRIRSFEHEEAG
                     IPPTVSFLDRLKIHTLDGYDVRAQVIALVGAHLAPSHLYKNRDNVGDGAFRRLARKLE
                     PELLYRVSRADCLGRTGDFATDAQEWFIAKVRALGVEEKPPKPILMGRHLLEMGLRPG
                     PEIGRIARAIYEMQLDGRVIDLEGALQVARGMLDPK"
     gene            2982..5033
                     /locus_tag="VFD06_08485"
     CDS             2982..5033
                     /locus_tag="VFD06_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN03613.1"
                     /translation="MTRRTFLARCSTSTLMGLAFTIPAALIAGLLLLYHYDMAQTSLS
                     QEGARYGQVLADPILGASQRYLREGSVPAIQAMMEDAGRNATIGDIALIGDDDRVVAS
                     NHRSWIGRPAEDLEIPGYTRSAEEARRTGMMQHRLRDRGAHLVLLAAVPPVMAVTPAA
                     PVAPSASTAGESVADGGILYMQIDQGRRLAEIWSGILKRGIASAVGIVAVSLLLYVWV
                     RAILARPMLEIAAYVRVLAAGGTAAPPRARGPLEVEALREDILGLAENLDAKERALDA
                     SHRLVREAERMESVASLAGALAHDFNNLLTGILGYSRLLLDRIGPGDPIRRQLAAIEA
                     SASRAADLTTRLLTFSRRAASRPAPVDPAEALAPAILALRDGLPEGIHLVVERDLELW
                     ATAIDSEQIRRVLAALGSNAVEAMGWGGTITIALLNRLLMESDCRGRLDARPGRFVVL
                     VVSDTGTGIDKEIRRRLFEPFVTTKKQAGGAGFGLATAYGLVKGHEGWIEVESEPGKG
                     TKFTVYLPACPPGTTVATADVPAAHQAEAPVLFPESAAVAAMAGEPAPVLTVLAVDDE
                     STVLALARDVLEMHGYKVLTARNGEEALRVFREHQASVALVLLDLTMPVMGGVECFRK
                     MKELDPHVRVVISSGFSSESTASEVLREGALDYLQKPYDIEHLARIVAKALARPLAAT
                     A"
     gene            5074..5358
                     /locus_tag="VFD06_08490"
     CDS             5074..5358
                     /locus_tag="VFD06_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN03614.1"
                     /translation="MEEALRVFREHQSEVALVVLDLTMPVMGGVECFRKMKDIDPRVR
                     VVISSGFSSENTAAEVLREGALEYLQKPYDIEDLARVVRKALTRPLAATA"
     gene            5508..6416
                     /locus_tag="VFD06_08495"
     CDS             5508..6416
                     /locus_tag="VFD06_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03615.1"
                     /translation="MRRMAWLMVILALGWMPALATETFLVPNTFTSDLTNLTYGYNLT
                     SSTLSSAAMQVSQGELRMTITGFTKKTVTIWLNGGSTSIDATLRPGSEAGTYELIANY
                     GAQGFVVFVYDATTWEILDIAHTYDLEIPEIAPTSDFEMLMQAMAEALDQVSADIEHR
                     QSVDAHERDLAAWLTILVAGPHIVRQDNVIQRKICGEAGYADCEYCCHNESVVDKSCL
                     RTATICVGNVQSYCGALVGTCGGISELEISAENACIEHDCRGLPGDPICSPSTQCLGY
                     CAPMCGLNSGICGGCARGFDRCCSFY"
     gene            6491..6871
                     /locus_tag="VFD06_08500"
     CDS             6491..6871
                     /locus_tag="VFD06_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HZN03616.1"
                     /translation="MKKFAAALMVLSLAAVPALANCDKHDSTDAKSAEFGKGPVKDVV
                     LTGYLTDSNCGAANANKAGAECAKKCIKGGAKVQLVADAKTYTLEKVDKVEKHLGHEV
                     KVTGQLDETAGIIRVASIEAVAKS"
     gene            7225..8901
                     /locus_tag="VFD06_08505"
     CDS             7225..8901
                     /locus_tag="VFD06_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN03617.1"
                     /translation="MSEARWRGIAWFTVLSIAAVAAINAAGIWSMAVARRGLQEDAAR
                     LLTLETAERASGLEAILARTRGELAFMAGSPVFFDLESALGSRDPSLARWRRLEAEGA
                     VLLFLRAHPEVMRASVLGTGGRPLLEAARRGGVPVLWMTSGEATAPPARRAIDPIERP
                     VEGRFAPRLGTRSVRGAVTVEATIDAKRLLPVDGTAAGGRVCRLTDSAGVLLAGFDGE
                     PAGRRAASDGGARRSASAQAAIDAEGWSAPSPWRLDCRESGPGTALALLDPLESRYRK
                     TLVLNLAAMSLAVVLGGFAIHQARRRRDMEAQAREEARVRDLERKLFHAERLGTVGRL
                     AAGMAHEINNPLEGMANYLDLARSDLERGDAASARRRLDGVQEGLRRIGSIVGQVLAH
                     SDPARAPMAPVDLGATARQAAEFVRSRPEFAQVRFQFDLPDGLPTVPGRQALLGQVFL
                     NIVLNACEAQPDGGEVRLAARAVAGGGVEIEIADRGPGIGRDEAPRVFEPFYSTKGST
                     GLGLSTCYAIVEQHRGTIEVEPRPGGGALFRVRMPAASSPPALRAEGARA"
     gene            8894..>10038
                     /locus_tag="VFD06_08510"
     CDS             8894..>10038
                     /locus_tag="VFD06_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN03618.1"
                     /translation="MPEARESRGSAAAGRRAPARGRVLVVEDEAYVRASLDEVLRSRG
                     FDVSLAGGVDEAMTHLATAPVDVVLADLRMPGGGGLELVRRAHAVHPGVPVVILTGHG
                     TVSSAVECMKAGAGDYLLKPVDPEALEVALDRALTARALEREVAYLRRAADGGEEPDG
                     PLGDSPAWRRVMEKVAAAAPTDSTVLLLGESGTGKEMVARQIHRLGRRAASAYVRVNC
                     AAVPLEMWESEFFGHRRGAFTGASADRDGRFRLAHKGTLFMDEIGAMPAPGQAKILRV
                     LQDGEFDRLGDEQPTRVDVRLIAATNSDLEAEVAAGRFRADLFYRLNVIRIDLPPLRE
                     RVEDIPLLARHFARDIAARLGRRAPSLSGSAADLLKSYHWPGNARELR"
BASE COUNT         1502 a         3444 c         3581 g         1511 t
ORIGIN      
        1 cctcgaccgg cgcgcgcgcg agcgtcaggg ccgcctgcag gccgccgaac ccgccgccga
       61 cgatgaccac gcgatgcgag cccacgaggg tgcgatggta cactcgccgc ctccgggaga
      121 cagatcgatg cgcatcgaga cgttcaagat ggaacggatg caatcgacgt acgagaacca
      181 ggtcgagtgc aacctgtcgg aaagcggcgt cgagccgatg cgtccggagg atctcctcga
      241 gggggacgcc gagcggctcg ccttcttgca gcagaagctc gggtactgcc agtcgaacgg
      301 cagcgaagag ctgcgcgatc agatcgccct cttctacccc ggcgcgaagc gggacaacgt
      361 cctggtcacg aacggcggct cggaggcgaa ctacgcctcc ttctggtcgc tcctcgagcg
      421 cggcgaccgg gcggcggtga tgctgccgaa ctacctccag acctgggggc tgtcgcgcgc
      481 ctacgcgggg aaggccgacc ccttccggat gatcgagcgc ccggacggcc gcggcgggcg
      541 gcgctggggg ctcgacatcg cgggcctgaa gcgggcggtg acgaagcgca cgaaggtcat
      601 cctggtcacc aacccgaaca atccgaccgg cggcgtgctc gacgaggaag agatggacgc
      661 cgtggtcgcg gtggcccgcg cgagccgcgc ctggctcgtg gtggacgaga tctaccgcgg
      721 cgcggagctc tcggggccga ccaccccgac cttctggggg cgctacgagc gcaccctcat
      781 cacctccggg ctgtcgaagg cgttcggcct gccggggctg aggatcggct gggtggtcgg
      841 gccgccggcg cagatcgcca agctctgggg ctaccacgac tacacgacga tcgccccggg
      901 gatgctctcc gaccggctgg cgcggcgcgc catgaccccg tcggtgcggg aggagatctt
      961 cgcccgcacg cgtggctacg tccggcgcta ctggccgatc gtcgacgggt tcgtgaagga
     1021 gcggtcggac tggctctcgg tcatcgcgcc ggtcgccggc gccatcgccc tcatccgcta
     1081 cgacctgccg atcgcctcga tcccgctcgt cgagcggctg cgcaccgaga aatcgaccct
     1141 cgtcgtggcg ggggaccaga ccggcatccc gaagcacatc cgcatcggct tcggctacgg
     1201 cgccgatccg tcggtgctgc acgacggtct gggacggatc gacgcgctga tgcggacact
     1261 ccggaccggc tcgcggaaga gcgccgccgg atcccgccgc gcggcgggga gcggggtgaa
     1321 gcgggcggcc gcgggcgggg cgcgcagggc ggccgccgcc cgctgaggcg gtcccgctac
     1381 ttcgggtcga gcatcccgcg cgccacctga agcgccccct cgagatcgat cacccgaccg
     1441 tcgagctgca tctcgtagat cgcccgggcg atccggccga tctccggccc gggccggagc
     1501 cccatctcca gcaggtgccg ccccatcagg atcggcttcg ggggcttctc ctccacgccg
     1561 agcgcccgca ccttcgcgat gaaccactcc tgcgcgtcgg tggcgaaatc ccccgtgcgg
     1621 cccaggcaat cggcgcgcga gacgcggtac aggagctccg gctcgagctt gcgcgccagc
     1681 ctccggaacg cgccgtcccc gacattgtcg cggttcttgt agagatggct cggcgcaagg
     1741 tgcgcgccga cgagcgcgat cacctgggcg cggacgtcgt atccatccag cgtgtggatc
     1801 ttgagtcgat cgaggaacga caccgtcggc gggataccgg cctcctcgtg ctcgaacgac
     1861 cggatgcgcc cctccaccac cgcggtcgtg gacggctttc cgaagtcgtg gcagaggacc
     1921 gccagcatca cggtggcttc cttggcgtac ggcagccccg ccgcctcgat gcgggcccgg
     1981 tcgatcgcca ggagggtgtg aatccagaca tccccctccg ggtgccattc cttctcctga
     2041 gggcagccga cggtggcgta gagctcgggg aaaagacgct ggatcgcgcc gagccggagc
     2101 ccccagtcga gcccgatcga cgggcgcgcc gccttcatca ggagcttctc catctccccc
     2161 cagacccgct cgcccggcag gtcgtcgagc ggcagggagc ggcacagcga aacggtcgcg
     2221 tcgtcgaagg aaaacccgag gcgggcggcg aactgcatgc cgcgcagcac gcgcagcgag
     2281 tcctcggcga aggtgtccgg atcgaccgcc cgcaggacgc gattctcgag gtcccccagc
     2341 ccgccccacg gatcgaggac ctcgccggtc agcggatcga ggagcagggc gttgacggtg
     2401 aagtcgcggc ggcgggccgc ctcctcgatc gacagctgcg ggtccccttc gaccgcgaaa
     2461 ccgcggtgac cacggccggt ccgctggtcg cgccgcggca ggctgacatc gatctcgatg
     2521 gccccctcgt tcgcggcgtc gggcccccgc ggcgtgacct tgtagacggc gaatgactcg
     2581 ccgaccaggt tgacgctccc gaaccggcgc aggaggaggg cgagctgctc cggggggaga
     2641 ccgtagattt caagatcgaa ctcgcactcc ccggtccccc ccgtctggcc gtcgttcagg
     2701 ctccggacgg tgtcccgcac gaacccgccg acgaggaagg cccggccgcc cgcctgctgc
     2761 gccgcggcgg cgattttccg cacggtcggg gggagaccgg cgagccggtc gtccgggagc
     2821 acgagcgcac gtttcattgc gtgagtatag ggtgccgcgc gacggtcgga tcgcttgcga
     2881 tcgccccggc cggtccttaa gttcagggcg atccgatgag cgtgcacagt gggcgtccgc
     2941 caaggctttg acggcatccg cgcgagcggg ggaaggggcc agtgacgcga cgcacgttcc
     3001 tggcgcgctg ctccacctcc accctgatgg ggctggcctt caccatcccg gccgccctca
     3061 tcgcggggct gctcctcctc taccactacg acatggcgca gacctccctg tcgcaggagg
     3121 gggcgcgcta cggacaggtg ctcgccgacc caatcctcgg agcgtcgcag cggtacctgc
     3181 gcgaggggtc ggtccctgcc atccaggcga tgatggaaga cgccggccgc aacgccacca
     3241 tcggcgacat cgcactcatc ggagacgacg atcgcgtcgt cgccagcaac catcggagct
     3301 ggatcggccg ccccgcggag gacctggaga tcccgggtta cacgcgctcc gccgaggagg
     3361 cgcgccgcac cggcatgatg cagcatcgcc tccgcgatcg cggcgcccac ctcgtcctcc
     3421 tcgcggcggt gcccccggtc atggcggtga ccccggcggc gccggtcgcc ccttccgcgt
     3481 cgacggcggg ggagagcgtc gcagacggcg gcatcctcta catgcagatc gaccaggggc
     3541 ggcggctcgc ggagatctgg tcggggatcc tgaagcgggg gatcgcctcg gcggtcggca
     3601 tcgtggccgt gagcctgctg ctgtacgtct gggtgcgggc gatcctggcg cggccgatgc
     3661 tggagatcgc cgcgtacgtg cgcgtcctcg ccgccggcgg caccgcggcg ccgccgcgcg
     3721 cgcgggggcc gctcgaggtc gaggcgctgc gcgaggacat cctcgggctc gccgagaacc
     3781 tcgacgccaa ggagcgggcg ctcgatgcgt cgcaccgcct ggtgcgcgag gcggagcgca
     3841 tggagagcgt cgccagcctt gccggcgccc tggcgcatga cttcaacaat ctcctgaccg
     3901 gaatcctcgg atactcgcgg ctcctcctcg accgcatcgg acccggagat ccgatccgcc
     3961 ggcagctcgc cgccatcgag gcctcggcga gccgcgccgc cgacctgacc acgcgcctgt
     4021 tgaccttcag ccggcgcgcc gccagccgcc ccgcgcccgt cgatccggcg gaagcgctcg
     4081 ctcccgcgat tctcgcgctg cgcgacggcc tgccggaggg gatccatctg gtggtcgagc
     4141 gcgacctgga gctctgggcc acggcgatcg attccgagca gatccgccgc gtcctcgccg
     4201 cgctcggcag caacgctgtc gaagccatgg ggtggggggg gacgatcacc atcgcgctcc
     4261 tgaaccgcct cctgatggag agcgactgtc gcgggcggct cgacgcgcga cccgggcgct
     4321 tcgtcgtcct ggtcgtctcc gacacaggca ccggcatcga caaggaaatc cgccgacgcc
     4381 tgttcgagcc attcgtgacc accaagaagc aggcgggagg ggccgggttc gggctcgcga
     4441 ccgcttacgg gctcgtcaag gggcacgaag gctggatcga ggtggagagc gagccgggga
     4501 aagggacgaa attcaccgtg taccttcctg cctgtccgcc cggcacgacg gtcgcgaccg
     4561 ccgacgttcc ggcggctcac caggccgagg ccccggtcct gttccccgaa tcggccgccg
     4621 tcgcggccat ggcgggcgaa ccggcgccgg tcctgacggt tctggcggtc gatgacgagt
     4681 cgaccgtgct ggcgctggcg cgcgatgtcc tcgagatgca tggctacaag gtgctgaccg
     4741 cccggaacgg cgaggaggcg ctgcgcgtct tccgcgagca ccaggcctcc gtggcgctcg
     4801 tcctcctcga tctcaccatg ccggtcatgg ggggagtcga gtgcttccgg aagatgaagg
     4861 agctcgatcc tcacgtgcgc gtcgtcatca gcagcggctt ctcctccgag agcaccgcct
     4921 ccgaggtgct gcgggaaggc gccctcgact atctgcagaa gccctacgac atcgagcacc
     4981 tcgcgcggat cgtcgcgaag gcgctggctc gtcccctcgc cgccaccgcc tgaaaagcga
     5041 aatgcggaag gcaaccctcc tgattgggag gcggtggagg aggccctgcg cgtcttccgc
     5101 gaacaccagt cggaggtggc gctggtggtc ctcgacctca ccatgccggt catgggcggg
     5161 gtcgagtgct tccgcaagat gaaggacatc gatccgcggg tgcgcgtggt catcagcagc
     5221 ggcttctcct ccgagaacac cgccgccgag gtcctgcgcg aaggagcgct cgaatacctc
     5281 cagaagccct acgacatcga ggacctcgcc agggttgtca ggaaggcgct cacccggccg
     5341 ctcgccgcta ccgcctgacc ccggccgatt tggttgctgt tcacgacacg cactgttccc
     5401 tccgaccgga gtgaccggtc ggaaggctca ttccaatatt ctcgctaacg gggcccgcag
     5461 gcgcccctcg ctcgctctga gagagcttat cgagaggagg acccatcatg agacgcatgg
     5521 catggctgat ggtcattctt gctctgggtt ggatgcccgc gctggcgacc gagacgtttc
     5581 tggtccccaa caccttcacc agcgatctca cgaacctcac ctatgggtac aacctgacct
     5641 cgtccaccct gagcagcgcc gcgatgcagg tctcccaggg cgagctgcgc atgacgatca
     5701 cgggtttcac gaagaagacg gtgaccatct ggctgaacgg cggctcgacg tcgatcgacg
     5761 caaccctccg gcccggcagc gaggcgggga cgtacgagtt gatcgcgaac tacggcgcgc
     5821 aagggttcgt ggtgttcgtc tacgacgcga cgacgtggga gatcctcgat atcgcgcaca
     5881 cctacgacct ggagattccc gagatcgcgc ccacctccga cttcgagatg ctgatgcagg
     5941 cgatggcgga ggcgctggat caggtgtcgg ccgacatcga acatcggcag tccgtcgacg
     6001 ctcacgaacg ggaccttgcc gcatggctga cgatcctggt tgcggggcca catatcgtcc
     6061 gacaggacaa cgtcatccaa cgtaagatct gcggagaagc cggttacgcc gactgcgagt
     6121 actgctgtca caacgagtcg gtcgttgaca agtcatgcct gaggaccgca acgatatgcg
     6181 tggggaacgt ccaatcgtat tgcggggcgc tggtgggaac ctgtggcggc atttccgagt
     6241 tggaaatttc cgctgagaat gcctgcatcg agcacgattg ccgagggttg cccggcgatc
     6301 ctatctgctc tccaagcacc caatgtttgg gatattgcgc cccgatgtgt gggctcaatt
     6361 cgggcatctg cggaggatgt gcacgcggtt tcgaccgttg ctgttcgttc tactgacgac
     6421 gtggccgcgg cgcgaagcgc agttccgttg tagaatcttc gggctgtttc cagcgcggag
     6481 gattcggatg atgaagaagt ttgctgcggc actcatggtt ctctccctgg cggccgtgcc
     6541 cgctcttgcc aactgcgaca agcacgactc gaccgacgcg aagtcggcgg agttcggcaa
     6601 gggaccggtg aaggacgtcg tcctgaccgg gtacctgacc gactcgaact gcggcgcggc
     6661 gaacgccaac aaggccggcg ccgagtgcgc gaagaagtgc atcaaggggg gcgcgaaggt
     6721 ccagctcgtc gccgacgcga agacctacac cctcgagaag gtggataagg tcgaaaagca
     6781 cctcgggcac gaggtgaagg tgaccgggca gctcgacgag accgcgggca tcatccgcgt
     6841 cgcgtcgatc gaggcggttg cgaagtcctg aagagcaggt cgccggacct cggtccggat
     6901 tcgaatcgaa ccaacccgaa gggccgcggc gacgcggccc ttcgtctttt ctgaggttcc
     6961 gcctcgccgt ttcccctcga ttgctctcat catagaacct atgttgaggg ccgcagccag
     7021 cggtcagacg gctcgcataa cagcgatgat aggtcccagg caggcgacca ttcgcagatc
     7081 tgcacgagcg acgtatctta ttgataaaag gctgcttctc aatcatcttt cggattgacg
     7141 gaagaaaccg accggttggt ccggggcttg ctgttccggg gcggccggac aggtgcaagt
     7201 gacacggtgc gttatagttc ggtcgtgagc gaggcgcgct ggcgggggat cgcgtggttc
     7261 accgtcctgt cgatcgcggc ggtcgccgcg atcaacgcgg ccggcatctg gagcatggcg
     7321 gtggcgcgcc gcggacttca ggaagacgcg gcccggctgc tgaccctgga gaccgcggag
     7381 cgggcgagcg ggctcgaagc gatcctggcg cgaacgcgtg gcgagctggc gttcatggcc
     7441 gggtctccgg tcttcttcga tctcgaatcg gcgctcggtt cgcgcgaccc gagcctggcg
     7501 cgctggcggc ggctggaggc agagggggcg gtccttcttt ttttgcgggc tcatcccgag
     7561 gtcatgagag cgagcgtttt gggaacgggc ggacgacccc ttctggaagc ggcgcgacgc
     7621 ggcggcgtcc cggtcctgtg gatgacctcc ggtgaggcga ccgctccgcc cgcgcggcgc
     7681 gcaatcgatc cgatcgagcg gccggtggaa gggcgcttcg ccccgcgcct cggaacgcgc
     7741 tcggtgcggg gggccgtgac cgtcgaggcg accatcgacg caaagcggct ccttccggtc
     7801 gacggcaccg ccgccggcgg gcgggtctgc cggctcaccg acagcgcagg ggtgctcctg
     7861 gccggcttcg acggcgagcc ggcgggtcgc cgcgccgctt cggatggggg cgcgcgccgc
     7921 tccgcctcgg cgcaggcggc gatcgacgcc gagggatggt cggccccgtc cccctggcgg
     7981 ctcgactgcc gcgagtcggg gccgggcacg gcgctggcgc ttctcgatcc gctcgagtcg
     8041 cggtaccgca agacgctcgt cctcaacctg gcggcgatgt cgctggcggt ggtcctcggc
     8101 ggcttcgcca tccaccaggc gcggcgccgg cgcgacatgg aggcgcaggc gcgcgaggag
     8161 gcccgggtgc gcgacctcga gcgcaagctg ttccacgccg agcgcctcgg cacggtggga
     8221 cggctggcgg ccggcatggc gcacgagatc aacaacccgc tcgaggggat ggcgaactat
     8281 ctcgacctgg cgcgcagcga cctcgagcgg ggcgacgccg cctcggcacg ccgccggctc
     8341 gacggcgtgc aggaggggtt gcggcgcatc gggtcgatcg tcggacaggt gctggcgcac
     8401 tcggatccgg cgcgggcgcc gatggcgccg gtggatctcg gcgccaccgc gcggcaggcg
     8461 gcggagttcg ttcgttcccg gccggagttc gcgcaggtcc gcttccagtt cgatctgccc
     8521 gacgggcttc cgaccgtccc tggccggcag gcgctcctgg gccaggtctt cctgaacatc
     8581 gtcctgaacg cctgcgaggc gcagccggac gggggggaag tgcgtctcgc cgcccgcgcc
     8641 gttgccggcg gtggcgtgga gatcgagatc gccgaccgcg gtcccggcat cggccgcgac
     8701 gaggcgccgc gggtgttcga gccgttctat tccaccaagg gctcgaccgg gctggggctg
     8761 tccacctgct acgccatcgt cgagcagcac cgcggcacca tcgaggtcga gccgcgcccc
     8821 gggggcgggg cgctcttccg cgtgcggatg ccggccgcgt cctcgccgcc ggcgttgagg
     8881 gcggagggcg cgcgtgcctg aggcgcgcga gtcgaggggg tccgccgcgg ccggccggcg
     8941 ggcgccggcg cggggacgcg tgctcgtcgt cgaggacgag gcgtacgtcc gcgcttcgct
     9001 cgacgaggtg ctgcgatcgc gcggcttcga tgtgagcctc gcgggaggcg tggacgaggc
     9061 gatgacccat ctcgccacgg ctcccgtgga tgtcgtgctc gcggatctgc ggatgccggg
     9121 aggcggcggt ctcgagctgg tgcggcgcgc ccacgccgtg catcccggcg ttccggtcgt
     9181 catcctgacg gggcatggca ccgtgtcgtc cgcggtcgag tgcatgaagg ccggagcggg
     9241 ggactacctc ctcaagccgg tcgatccgga ggcgctcgag gtggccctcg accgggcgct
     9301 gaccgcccgc gccctggagc gcgaagtggc ctacctgcgc cgcgccgccg atggcgggga
     9361 ggagccggac ggaccgctcg gcgacagccc ggcctggcgc cgggtgatgg agaaggtcgc
     9421 cgccgccgcg ccgaccgact cgacggtgct cctgctcggc gagtcgggca ccggcaagga
     9481 gatggtggcg cggcagatcc accggctcgg ccgacgcgcc gcgagcgcgt acgtgcgggt
     9541 gaactgcgcc gccgtgccgc tcgagatgtg ggagagcgag ttcttcggtc atcggcgcgg
     9601 tgcgttcacc ggggcctcgg ccgatcggga cggccgcttc cgcctggcgc acaaggggac
     9661 gctcttcatg gacgagatcg gcgcgatgcc ggcccccggc caggcgaaga tcctgcgcgt
     9721 cctccaggac ggcgagttcg accggctcgg cgacgagcag ccgacgcgcg tcgacgtgcg
     9781 gttgatcgcc gccaccaaca gcgacctcga ggcggaggtc gcggcggggc gcttccgcgc
     9841 cgacctcttc taccgcctga acgtcatccg catcgacctg ccgcccctgc gtgagcgggt
     9901 cgaggacatc ccgctcctgg cgcgccactt cgcccgcgac atcgcggccc gtctcggccg
     9961 tcgcgcgccg tcgctctccg ggagcgccgc ggatctcttg aagtcgtacc actggccggg
    10021 gaatgcccgc gagctgcg
//
LOCUS       DASRIQ010000355         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3263287, whole genome shotgun sequence.
ACCESSION   DASRIQ010000355 DASRIQ010000000
VERSION     DASRIQ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3263287"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFD06_08515"
     CDS             <1..373
                     /locus_tag="VFD06_08515"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03619.1"
                     /translation="GMGEGTYYWRVATAGRQGLESAFSEIRRFKVDGGRERAADDTVP
                     PDLMIAEFLPSGHLLIINGRTEPGSMLSVEGQSVDVYDDGTFTAVIRMKHEGKNDVAI
                     VAQDAAGNETRLRKSVFVESY"
     gene            435..1295
                     /locus_tag="VFD06_08520"
     CDS             435..1295
                     /locus_tag="VFD06_08520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN03620.1"
                     /translation="MAARALQRLSRLAPIAGWALYLAPAGGGPVSPHPVACRRAPLFR
                     GLAPGPGGRIPGRSTVPLRCRGRLVGVLALHPRGPGHRLPARQVRAAAVLLEPLAVAL
                     DAALLLRRSEELSVTDDLTHLYNVRYQNAALRREVSRSRRYRIPVSVIFLDLDGFKSV
                     NDRHGHLWGSRTLIEVGAVLRSVVREIDVVSRFGGDEFTVILPQTGAAGARVIAERLR
                     QRIASTTFLRAHGLEVRVTASLGIAAYPDHGRTEEDLVARADQAMYAAKAAGKNRVQT
                     ARTAPIPAIA"
     gene            1438..2469
                     /locus_tag="VFD06_08525"
     CDS             1438..2469
                     /locus_tag="VFD06_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZN03621.1"
                     /translation="MAWSPRSMLSLFSNDLAIDLGTATTLVYAKGKGIVLCEPSIIAV
                     NQRTGKVEAVGKEAKEMLGRTPANIIAIRPMKDGVIADFEHTEKMLDYFIKKAHNRSL
                     GVRPRIVICVPSDITQVEKRAVKDSAYRAKASEVFLVEEAMAAAIGAGLPVTEPTGNM
                     IVDIGGGTTDVAVISMAGIVYSRSVRIAGNRMDEDIIQYIKRKYNLLVGERTAEEIKI
                     NVGSAFPLDEELTMEIKGRDLVEGIPKTLVISDEEIREALSETVSNIIEAVRVALEQT
                     PPELSADIVDKGIVLTGGGSQLKNLDKRLREETGLPVSMADDPQSSVVLGTGKMLDDF
                     NLLRKIAID"
     gene            2536..>3240
                     /gene="mreC"
                     /locus_tag="VFD06_08530"
     CDS             2536..>3240
                     /gene="mreC"
                     /locus_tag="VFD06_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZN03622.1"
                     /translation="MPRVVSSQRKPSLLLFLLLSMHLVLMASEVRGVRGGSLLEDTLL
                     TLFSPFLRGASSMTSGVAGTWQRYGGLRGVEEENRRLRAQVGTLLLQAREAEEARREA
                     ERLRALLDLRDTTHPGAVAARVIARGGAGSARVLLVDRGSNQGVARQQPVITPRGAVG
                     RVVEAAPGVAKVLSIVDPNSGVAALVQRTRVQGVLVGEGEDSCRLEFVSDDAPVEVGD
                     VVVTSGLDAIFPKGILL"
BASE COUNT          479 a         1119 c         1171 g          471 t
ORIGIN      
        1 ggggatgggg gaggggacct actattggcg cgtcgccacc gccggccggc aggggctgga
       61 gagcgccttc tccgagatcc gccggttcaa ggtggacggc gggcgcgagc gcgccgccga
      121 cgacaccgtc ccgcccgatc tgatgatcgc cgagttcctc ccgagcgggc acctgctcat
      181 catcaacggc cgcaccgagc ccggctcgat gctctcggtc gagggccagt cggtcgacgt
      241 ctacgacgac ggcacgttca cggccgtcat ccggatgaag catgaaggga agaacgacgt
      301 ggcgatcgtc gcccaggacg cggcggggaa cgagacgcgc ctgcgcaaga gcgtcttcgt
      361 cgagtcctac tagaacgcac gcggctctgc gcgccatcgt gcgggtgacc gcgacggaga
      421 ccgatccgcg tcgcttggcg gcgcgcgccc tgcagcgcct gtcgcgcctc gcgccgatcg
      481 ccggatgggc tctgtacctg gcgcccgccg gcggcggccc ggtgtcgccg cacccggtcg
      541 cctgccgccg cgcgccgctg ttccgcggtc tcgcccccgg tccgggagga cgcatccccg
      601 ggcgatcgac cgtcccgctg cgctgccgcg gccgcctcgt gggggtcctg gccctgcacc
      661 cccgcggccc gggacaccgc ctgccggcgc gccaggtgcg ggccgccgcc gtcctcctcg
      721 agccgctggc ggtggcgctc gacgccgcgc tcctgctgcg ccgctccgag gagctgtcgg
      781 tcaccgacga cctgacgcat ctctacaacg tgcgctacca gaacgccgcg ctgcggcgcg
      841 aggtctcccg atcgcgacgc taccgtatcc cggtgtcggt catttttctc gatctcgacg
      901 gcttcaaatc ggtgaacgat cgtcacggtc acctgtgggg gagccgcacg ctcatcgagg
      961 tcggggcggt gctccggagc gtggtgcggg agatcgacgt cgtgtcgcgc ttcggcggcg
     1021 acgagttcac ggtgatcctc ccgcagaccg gcgccgcggg ggcccgggtg atcgccgagc
     1081 ggctccggca gcggatcgcc tcgaccacct tcctgcgggc gcacggcctc gaggtgcggg
     1141 tgaccgcgag cctcgggatc gctgcctacc cggaccacgg ccgtaccgag gaggacctcg
     1201 tggcccgcgc cgaccaggcg atgtacgcgg ccaaggcggc ggggaagaat cgggtccaga
     1261 cggcccgaac cgccccgatc ccggcgatcg cgtgagccga aaaaccctcc ggggcggtca
     1321 aggatttgac tttgcgatga cggaaaccct atattgaggc ccggtttggg actcgcgtaa
     1381 cttgttggaa cgtagtgcct tgcgcgctgc ggcgaggagg agacggtggg ggcgacgatg
     1441 gcctggagtc cgcggtcgat gctgagcctg ttctcgaacg acctggcgat cgacctcggg
     1501 accgcgacga cgctggtgta cgccaagggg aaggggatcg tcctgtgcga gccctcgatc
     1561 atcgccgtca atcagcgcac cggcaaggtc gaggcggtcg gcaaggaggc caaggagatg
     1621 ctcggccgca cgccggccaa catcatcgcc atccgcccga tgaaggacgg cgtcatcgcc
     1681 gacttcgagc acaccgagaa gatgctcgac tacttcatca agaaggcgca caaccgctcg
     1741 ctcggcgtgc gcccgcgcat cgtcatctgc gtcccgtccg acatcaccca ggtggaaaaa
     1801 cgcgcggtga aggattcggc gtaccgcgcc aaggcgtcgg aggtcttcct ggtcgaggag
     1861 gcgatggcgg cggcgatcgg cgccggcctg ccggtgaccg agccgaccgg aaacatgatc
     1921 gtggacatcg gcggcggcac caccgacgtc gcggtgatct cgatggccgg gatcgtctac
     1981 tcgcggtcgg tccgcatcgc cggcaaccgc atggacgagg acatcatcca gtacatcaag
     2041 cgcaagtaca acctgctcgt cggcgagcgc accgccgagg agatcaagat caacgtcggc
     2101 tccgccttcc cgctcgacga ggagctgacg atggagatca aggggcgcga cctcgtcgag
     2161 gggatcccga agacgctggt gatctccgac gaggagatcc gcgaggcgct gtcggagacc
     2221 gtctccaaca tcatcgaggc ggtgcgcgtc gccctggagc agacgccccc cgagttgtcc
     2281 gccgacatcg tcgacaaggg gatcgtcctc accggcggcg gctcgcagct gaagaacctc
     2341 gacaagcgac tgcgcgagga gaccgggctg ccggtgtcga tggccgacga cccgcagtcg
     2401 tcggtggtgc tcggcaccgg caagatgctc gacgacttca acctgctgcg caagatcgcc
     2461 atcgattagg agaccacggg ccggcgaccg gcgccacctc ccgggcggcc gcgtcctccg
     2521 ggccgggatg agccgatgcc ccgggtcgtg tcgtcgcaac gcaagccgtc actcctcctc
     2581 ttcctgctgc tgtcgatgca cctcgtcctg atggcgtcgg aggtgcgcgg cgtccgcggc
     2641 ggatcgctcc tggaggacac gctcctcacg ctcttctcgc cgttcctgcg gggggcgtct
     2701 tccatgacct ccggcgtcgc cggcacctgg cagcgctacg gcggcctgcg cggcgtggag
     2761 gaggagaacc ggcggctccg ggcccaggtc ggcacgctcc tcctgcaggc gcgcgaggcg
     2821 gaggaggccc gacgcgaggc ggagcgcctg cgcgcgctcc tcgacctgcg cgacaccacc
     2881 caccccggcg cggtcgcggc gcgggtcatc gcgcgcggcg gcgcggggtc ggcgcgcgtg
     2941 ctcctggtgg atcgcggctc gaaccagggg gtggcgcgtc agcagccggt gatcacgccg
     3001 cggggcgcgg tcgggcgcgt cgtcgaggcg gcgccggggg tcgcgaaggt gctgagcatc
     3061 gtcgatccga actccggggt cgccgccctc gtgcagcgca cgcgcgtgca gggggtgctg
     3121 gtcggcgagg gggaggacag ctgccggctg gagttcgtct ccgacgacgc cccggtcgag
     3181 gtgggggacg tggtggtgac ctcgggactc gacgccattt ttccgaaggg catcctgctg
//
LOCUS       DASRIQ010000356         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2473003, whole genome shotgun sequence.
ACCESSION   DASRIQ010000356 DASRIQ010000000
VERSION     DASRIQ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2473003"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /gene="asnB"
                     /locus_tag="VFD06_08535"
     CDS             <1..1864
                     /gene="asnB"
                     /locus_tag="VFD06_08535"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN03623.1"
                     /translation="FDGRPADPGVLAAMGASLAHRGPDGDGTFRDGGLGLAHRRLSIL
                     DIAGGAQPMTTTDGDAILVFNGEIYNYVELREGLRRRGHPLATHSDTEVILHLYEEQG
                     PDCVQELRGMFAFALWDRRLGRLVLARDRVGIKPLFYAQSDRGLVFGSEIKALLLHPD
                     VDRSLDLQGLGDFLTYQYIPSPRTGFARIRKLPAAHVLVAERGRVSIRRYWSLPEEPS
                     RRSGQEWREELRATVAEAVRLHMRSDVPVGALLSGGIDSSLVVAHAAAAAEGPLRTFT
                     IGFRDRGFSEAPFARLVSERYGTRHLEHVLEPQRIEDLPALVAHFDEPFGDPSAVPTS
                     LIARIAARDVKVCLSGDGGDEGFAGYDAYLSARRLRPADLVPLPARRALLAPMERRLP
                     EWVPGKGLLRFLGSGPADRYAEIMGGVDAGTREWLLHPDFLRATGGEPPYALFRRLHA
                     SLPGRDEVERLQRLDVETYLPDDILVKADRTSMLHSLELRVPLLDHKVLELAFRMPTA
                     LKIRRGRGKAILRTAFADRLPAGILARGKQGFGLPLAAWLRGDLLGLVRDVFGDARTR
                     TRGLLDPRGLDRLLASHLRGRRDLSGEIWLALVLELWFRERIDSPPRAAAARTA"
     gene            1875..>2425
                     /locus_tag="VFD06_08540"
     CDS             1875..>2425
                     /locus_tag="VFD06_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN03624.1"
                     /translation="MRIAYLHYLVDDDTGRHHVRQFAAAAASLGHVIDVRGMNLAPEG
                     NAGGDGDATARLRSALKQRFGRYLHEPKELLWNARYVGRETDLLRELRPDVLLVRDHL
                     LTASCVPVARRLALPLVLELNSPADEGRLYLGEYLHFPRVARAIERYKLRRADAITAV
                     SSTLKRLLVDTYGLPEGRITVVP"
BASE COUNT          313 a          917 c          838 g          357 t
ORIGIN      
        1 gttcgacggc cggccggccg atcccggcgt gctcgccgcc atgggggcaa gcctcgcgca
       61 tcgcggcccg gacggcgacg ggacgttccg cgacggaggg ctggggctcg cccaccgtcg
      121 gctgtcgatc ctcgacatcg cgggcggggc ccagccgatg accacgaccg acggcgacgc
      181 catcctcgtc ttcaacggcg agatctacaa ctacgtcgag ctccgcgaag gacttcggcg
      241 tcgcggccat ccgctcgcga cccacagcga cacggaggtg atcctgcacc tctacgagga
      301 gcagggaccc gactgcgtcc aggagctgag ggggatgttc gccttcgcac tgtgggatcg
      361 gcgcctcggg cgtctcgtcc tggcccgcga tcgagtcggc atcaagccgc tcttctacgc
      421 ccagagcgac cgcggcctgg tgttcggctc ggagatcaag gcgctgctgc tccatccgga
      481 cgtcgaccgg tcgctcgatc tccaggggct cggcgacttc ctgacctacc agtacatccc
      541 atcgccgcgc accgggttcg cgcgcatccg caaactcccg gccgcccacg tcctggtggc
      601 ggagcgcggc cgcgtctcga tccggcgcta ctggagcctg ccggaggagc catcgcgccg
      661 ctccgggcag gagtggcggg aggagctccg cgccaccgtc gcggaggcgg tccgcctcca
      721 catgcgcagc gacgtgccgg tcggggcgct cctgtcgggc ggcatcgaca gcagtctcgt
      781 ggtcgcccac gcggccgccg ctgccgaagg tccgctgcgc acgttcacca tcgggttccg
      841 cgaccgcggc ttctccgaag ccccgttcgc gcgtctcgtg tcggagcggt acggcacgcg
      901 gcacctggag cacgtgctgg agccgcagcg gatcgaggac ctgcccgccc tcgtcgccca
      961 tttcgacgag ccgttcggcg atccctccgc cgtcccgacg agcctgatcg cgcgcatcgc
     1021 ggcgcgcgac gtcaaagtct gcctgtcggg ggacggcggg gacgagggtt tcgccggata
     1081 cgacgcgtac ctctccgccc ggaggctgcg gccggccgat ctcgtgcccc tgccggcgcg
     1141 ccgggcgctc ctcgccccga tggagcgtcg cctgcccgag tgggtgcccg gaaagggact
     1201 gctgcgcttc ctcggatccg gccccgccga ccgctacgcg gagatcatgg gaggagtgga
     1261 tgccggcaca cgcgagtggc tgctgcaccc cgacttcctg cgcgcgaccg gcggcgagcc
     1321 gccgtacgcc ctgttccgcc ggctccacgc ctcgctcccg ggacgcgacg aggtcgagcg
     1381 gctgcagcgg ctggacgtcg agacgtacct tcccgacgac atcctcgtca aggcggaccg
     1441 cacgagcatg ctgcactcgc tcgagctccg cgtgccgctt ctcgatcaca aggtcctcga
     1501 gctggcgttc cgcatgccga ccgcgctgaa gatccgccgc ggacgcggca aggccatcct
     1561 gaggaccgcc ttcgcggacc gtctgccggc cggaatcctc gcgcgcggca agcagggatt
     1621 cggcctgccg ctcgcggcct ggctgcgcgg cgacctcctg gggctcgtgc gcgacgtctt
     1681 cggcgacgcg cggacccgca cgcgcggact cctcgacccg cgcggtctcg atcgcctgct
     1741 cgcctcgcac ctgcgcggcc ggcgcgacct gagcggcgag atctggctgg ccctcgtcct
     1801 cgagctgtgg ttccgggagc ggatcgacag cccgccgcgc gccgccgcgg cgcgcaccgc
     1861 ctgatcggcc gtcgatgcgg atcgcctacc tgcactacct cgtcgacgac gacaccggcc
     1921 gtcaccacgt ccggcagttt gcggcggcgg ccgcctccct cggccacgtc atcgacgtcc
     1981 gcggcatgaa cctcgcgccg gaagggaacg cgggcggcga cggcgatgcg acggcgcggc
     2041 tgcgatcggc gctgaagcaa cgcttcgggc ggtatctcca cgagccgaag gagctcctct
     2101 ggaacgcccg ctacgtcggt cgcgagaccg atcttctgcg ggagctgcgg ccggatgtgc
     2161 tcctggtgcg cgatcacctc ctgaccgcct cctgcgtccc ggtggcgcgg cgcctggcgc
     2221 tgccgctggt gctggagctg aattccccgg ccgacgaggg acggttgtac ctcggcgagt
     2281 acctgcactt cccgcgcgtc gcgcgggcga tcgagcggta caagctgcgg cgcgccgacg
     2341 ccatcaccgc cgtctcctcg acgctcaagc gcctgctcgt ggacacctac ggcctgcccg
     2401 aggggcgcat cacggtcgtc ccgaa
//
LOCUS       DASRIQ010000357         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3007030, whole genome shotgun sequence.
ACCESSION   DASRIQ010000357 DASRIQ010000000
VERSION     DASRIQ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3007030"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1408)
                     /locus_tag="VFD06_08545"
     CDS             complement(191..1408)
                     /locus_tag="VFD06_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN03625.1"
                     /translation="MSDLKDQLASLRIDRDRPPGGGAGRWLGLAAVALVLAAAGTWLL
                     LGGGLASLRAVEIETVRPKVQSGSGPAPGSAILTASGYVVPRRKAVVSAKIQGRLMEL
                     RVEEGSRVKQDEIIARLENQDYVAQVSSARAGVQRAEADLAEQQRQLRLSRQLESENV
                     LSRDAREAAESRVRMAEAALGQAKALLEVSDAQYRNTLIRAPFAGVVLKKMAEVGESV
                     APIPPGVNISSSSGAIVALADLDTLEVEADVSESNVARLDDAQPAEVSVEAFPDRTYK
                     AVLRQIIPTADRTRATVMVKVTILDKDPDLKPEMNAKVNFLEKEAAAEAGPQAAAAPP
                     TITVPNDVVVDRGGAATVFEVKDGRARARAVVTAGTREGQVVVREGLQGSETLVLRPP
                     TTLKDGDAVRVRS"
     gene            complement(1419..1859)
                     /locus_tag="VFD06_08550"
     CDS             complement(1419..1859)
                     /locus_tag="VFD06_08550"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1728..1730),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZN03626.1"
                     /translation="MVPVGAQAPAFDVKDHHGQDTRLDDFRGRRVVLWFYPKADTPGU
                     TLEGCGYRDLKTQFDAKGAVILGVSFDTVEENDAFARKFSFPYPLLCDTERAIGMAYG
                     ACDNPTDRTARRISYVIGPDGRIEHAFPKVDPKRHPAEVLALLP"
     gene            complement(1921..>3381)
                     /locus_tag="VFD06_08555"
     CDS             complement(1921..>3381)
                     /locus_tag="VFD06_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HZN03627.1"
                     /translation="GGSGGRVIFTGMAIGAGVKLLLAIVFLCPGSLAQSLPFLPKGEV
                     ALEVAPALIGVGYILGYRQSAILVAGSIVASLALIPLIAWFGGGLPTPLYPETQNTIA
                     SMTAGQIWGRYVRYIGAGAVATAGILTVLKNLPVMAGAARAVLQGLLRTKEEAGAAAA
                     VPATDRDLPPAFIAAGVLVVVAVSAFVPGVFAGGMGPGPRLVCAAGVALFGILFVAVA
                     ARIVGIVGVTSQPTSGITLVTLLGIASLFAAAGWTTSAARAAVLTVGTIVAVAASKAG
                     DISQDLKTGWLVGATPARQQLGQLLGAAFACFGVAATVLLLGNAYTFGSTEVPAPQAT
                     LMKTIIEGVLAGALPWGLVLSGAGIALSAILCGVSGLAFAIGVYLPLASMAAIYVGGA
                     IRAVAERRAAGSPGAGTGPAEARPGVLAASGLVAGEGLAGVLVAGLVASGAIAKSRPP
                     LLEGDLGRLAALGLVAAIAFFLLRAGRRTGPSASGA"
BASE COUNT          490 a         1246 c         1202 g          443 t
ORIGIN      
        1 gtcgtcttcc ccgatccgga ggggcccatg agggcgagga actccccctc ctgaacgtcg
       61 aggtcgagcc cgtcgagcac cgtgatcgcc agggcgtcgc gccggtacac cttgcgcacg
      121 ccgcgcactt ccaccatcgc catgccgtgt tcctccctct gaagttccgc cgcgtccccg
      181 cggacgtcgc ctacgaccgg actcgcaccg cgtcgccgtc cttgagcgtc gtcggcgggc
      241 gcaggacgag cgtctccgat ccctgaagcc cctcccggac caccacctgc ccttcgcgcg
      301 ttccggcggt gaccacggcg cgggcgcgcg cccggccgtc cttcacctcg aacaccgtcg
      361 ccgccccgcc gcgatcgacc acgacgtcgt tcggcaccgt gatggtcggc ggcgccgcgg
      421 ccgcctgcgg ccccgcctca gccgctgcct ccttctcgag gaagttcacc ttggcgttca
      481 tctccggctt gaggtccggg tccttgtcga ggatggtgac cttcaccatc accgtggcgc
      541 gggtccggtc ggcggtcggg atgatctgcc gcagcaccgc cttgtaggtc cggtccggga
      601 acgcctcgac gctgacctcc gccggctgcg cgtcgtcgag gcgcgcgacg ttcgactcgc
      661 tgacgtccgc ctcgacctcg agggtgtcga ggtcggccag cgccacgatg gcgccggacg
      721 acgacgagat gttcacgccg ggggggatcg gcgcgacgct ctcgccgacc tccgccatct
      781 tcttgagcac cacgccggcg aacggcgccc ggatgagggt gttcctgtac tgggcgtcgc
      841 tgacctcgag gagcgctttc gcctgcccga gcgccgcctc cgccatccgc acgcggctct
      901 ccgccgcctc gcgcgcgtcg cgcgacagga cgttctcgct ctcgagctgt ctcgacagcc
      961 gcagctggcg ctgctgctcg gccaggtccg cctcggcccg ctgcaccccg gcgcgcgcgc
     1021 tcgacacctg ggcgacgtaa tcctggttct ccaggcgggc gatgatctcg tcctgcttga
     1081 cccggctccc ttcttcgaca cgaagctcca tgagccgccc ctggatcttg gcgctgacca
     1141 ccgccttgcg gcgcggcacg acgtatccgg aggcggtcag gatggcgcta cccggggcgg
     1201 ggccggagcc gctttggacc tttggccgga ccgtctcgat ctcgacggcg cgcagggacg
     1261 ccagcccgcc gccgaggagg agccaggtcc ccgcggccgc cagcacgagc gcgaccgccg
     1321 cgagcccgag ccagcggccg gctccgccgc cgggcgggcg atcgcgatcg atccgcagcg
     1381 aggcgagttg atccttgaga tcgctcaacg ggctcgactc agggcaggag ggcgaggacc
     1441 tccgccgggt gccgcttcgg atcgaccttg gggaacgcat gctcgatccg gccgtccggg
     1501 ccgatgacgt aggagatgcg gcgcgcggtg cgatcggtgg ggttgtcgca ggcgccgtac
     1561 gccatgccga tcgcccgctc cgtgtcgcac agcagcggat aggggaacga gaacttccgc
     1621 gcgaacgcat cgttctcctc gaccgtgtcg aacgacacgc cgagaatgac ggctcccttc
     1681 gcgtcgaact gcgtcttcag gtcgcggtac ccgcaacctt cgagcgttca tccgggtgtg
     1741 tcggccttcg gatagaacca caggaccacg cggcgcccgc ggaagtcgtc gaggcgggtg
     1801 tcctggccgt ggtgatcctt cacgtcgaac gcgggggcct gggccccgac cggaaccatg
     1861 cgcacctcct cacaggggtt gagtcggagc gcagaggcta tcacccgctc gggggagggc
     1921 tcaagccccg gacgcggagg gcccggtgcg gcggccggcc cggagcagga agaacgcgat
     1981 cgccgccacg aggcccaggg ccgcgagccg gccgagatcc ccttcgagga gcggcggccg
     2041 cgacttggcg atcgcccccg aggcgacgag cccggcgacc agcacgccgg ccagtccctc
     2101 tccggcgacg agcccggagg cggccagcac gccggggcgc gcctccgccg gtcccgtccc
     2161 cgcgcccgga gatcccgcgg cgcgccgctc cgccacggcc cggatggcgc cgccgacgta
     2221 aatcgccgcc atcgacgcga gcggaagata gacgccgatg gcgaacgcga ggccggagac
     2281 gccgcagagg atcgccgaca gggcgatgcc cgcccccgac aggacgagcc cccacgggag
     2341 cgcccccgcc agcaccccct cgatgatcgt cttcatgagc gtcgcctgtg gcgccgggac
     2401 ttccgtcgaa ccgaacgtat aggcgttccc gaggagcagg acagtggcgg cgacgccgaa
     2461 gcaggcgaag gcggcgccga ggagctggcc gagctgctgg cgggccgggg tggcgccgac
     2521 gagccagccg gtcttcaggt cctgggagat gtcgccggcc ttcgacgcgg cgacggcgac
     2581 gatggtgccg actgtcagga ccgcggcgcg cgccgcggac gtggtccatc cggcggcggc
     2641 gaagaggctg gcgatgccga gcagggtcac gagcgtgatg ccggaggtcg gctgcgaggt
     2701 gacgccgacg atgccgacga tgcgggccgc caccgcgacg aacaggatgc cgaagagggc
     2761 cacgccggcg gcgcagacga gccggggacc cggccccatc ccgccggcga acacgccggg
     2821 gacgaacgcc gacaccgcga cgacgacgag cactccggcc gcgatgaagg ccggaggcag
     2881 gtcgcggtcg gtcgccggca ccgccgcggc ggctcccgcc tcctccttcg tccgaaggag
     2941 cccctgcagg acggcgcgcg cggcccccgc catcaccggc aggttcttca ggacggtcag
     3001 gatccccgcg gtcgcgaccg cgccggcgcc gatgtagcgg acgtagcggc cccagatctg
     3061 tccggcggtc atcgaggcga tggtgttttg cgtctccggg tagagcggcg tcggaagacc
     3121 gccgccgaac caggcgatga gcgggatgag ggccagcgag gcgacgatcg atccggccac
     3181 gaggatcgcc gactgccggt atccgaggat gtagccgacg ccgatgagcg ccggcgccac
     3241 ctcgagggcc acttcaccct tggggaggaa aggcagcgac tgcgcgagac tcccggggca
     3301 caggaagacg atcgccagga gcagcttgac gccggcgccg atcgccatgc cggtgaagat
     3361 gacgcgccca ccgctccctc c
//
LOCUS       DASRIQ010000358         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2762011, whole genome shotgun sequence.
ACCESSION   DASRIQ010000358 DASRIQ010000000
VERSION     DASRIQ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762011"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1023
                     /locus_tag="VFD06_08560"
     CDS             118..1023
                     /locus_tag="VFD06_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03628.1"
                     /translation="MTEVRFRRAARSLLSAVVSLSFALLLAAPLSRSWGQTPPEPPST
                     PPESPPTVPPDSPPPPAEGSEAEPLVPAASDPGETPGGALGYFMTMRDYRTIRQLKSI
                     LTPQANAAYDHDSVAYNGRKGVRLASWDYKEPAPKPGAVSHTSTVRSLWDEQGEAVEL
                     RTETVKLQRDPTGPWRVASLQKTAAENLRFKEAIPGVTSLRMVLRAWRLQDTQAARSL
                     LTDAFLKRLEARGDGLAALFAPAEGKRHAAFRIKGYEAKSPTQGIARVGLVDVPPGRP
                     GPLEGAPRTLTLVKRGSRWLVDDWR"
     gene            1094..2497
                     /gene="purF"
                     /locus_tag="VFD06_08565"
     CDS             1094..2497
                     /gene="purF"
                     /locus_tag="VFD06_08565"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZN03629.1"
                     /translation="MKNHGFKDECGVFAVFGHPEAANLVYLGLHALQHRGQESAGIVS
                     SNGQRLLEEKGMGQVGRVFKRKNLLRLKGTHAIGHVRYSTAGKSALVNAQPFRIDSCK
                     GEIAIGHNGNLVNAEALRKSLEAAGSIFRSSSDTEVVLHLIAKAPQSDLEAAIVHALK
                     QVEGAYSLVFITPDRTIGVRDPRGFRPMALGKAGDAHVLTSESCALDLIDGAFVREVE
                     PGEMVVISAQGVKSYRPFPAAPPSQCIFEHVYFSRPDSFVFGRSVHEVRKALGRELAR
                     EQPVEADVVVPIPDSGVYAALGFAEGSGLPLEFGFVRNHYIGRTFIEPRQSIRHFGVK
                     LKLNPVKRLLEGRRVVLIDDSIVRGTTSRKIVAMVRAAGAREVHMRISCAPTVGPCYY
                     GVDTPRRKDLIAANHTVEEIRRFIEADSLGYLSLEGMARAVGGAKGFCTSCYTNEYPV
                     PIPDEAAVRVAAPPRLR"
     gene            2512..>3332
                     /locus_tag="VFD06_08570"
     CDS             2512..>3332
                     /locus_tag="VFD06_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HZN03630.1"
                     /translation="MTDPFATWDSDRLAITTLKMLAVDAVEKAKSGHPGMPMGMAEVA
                     YMLWTKHLRFDPERSDWQDRDRFVLSAGHGSMLLYGLLHLSGYDLPLEELKRFRQWES
                     KTPGHPEYRHAPGVETTTGPLGQGLGNSVGMAIGAKRLAARFNTSDRRIIDHRVWALV
                     SDGDMMEGVGSEAASVAGHLKLGNLTLLYDDNHITIEGSTDLAFSEDVGKRFEAYGFR
                     VWRIDGHDLAAIGRTLDEAAKERARPGIVICRTHIGHGAPHKQDTAEAHGSPLGP"
BASE COUNT          512 a         1221 c         1132 g          467 t
ORIGIN      
        1 cgattcgatc cgcaacgagc cgcggcaaat ccaccgactc tgcttccccg tcgagatgag
       61 gacggtcgtc gagaggcgac tccgcccctt catctcctcc gagagacaat gacaccgatg
      121 accgaggtcc gtttccgtcg cgccgcccga tccctccttt ccgccgtggt ctccttgtcg
      181 ttcgccctgc tcctcgcggc gccgctgtcg cgctcctggg gacagacgcc gccggagccc
      241 ccgtccaccc cgccggagtc gccgcccacg gtcccgcccg attcgccgcc tccgcccgcc
      301 gagggatcgg aggccgagcc gctggtgccg gccgcgagcg acccggggga gacgccggga
      361 ggcgcgctcg ggtacttcat gacgatgcgc gactaccgca ccatccgtca gttgaagtcg
      421 atcctcacgc cccaggcgaa cgccgcctac gatcacgact cggtcgccta caacggccgc
      481 aagggggtgc ggctggcgtc ctgggactac aaggagccgg cgccgaagcc gggggcggtg
      541 tcgcacacct cgacggtccg cagcctgtgg gacgagcagg gggaggcggt cgagctgcgc
      601 accgagacgg tcaagctgca gcgcgacccg accgggccgt ggcgcgtggc ctccctccag
      661 aagaccgccg cggagaacct ccggttcaag gaggcgatcc cgggggtgac ctcgctccgg
      721 atggtgctgc gcgcctggcg acttcaagat acgcaggcgg cccggtcgct cctgaccgac
      781 gcgttcctga aacggctcga ggcgcgcggc gacggtctcg ccgcgctgtt cgccccggcc
      841 gaggggaagc gccacgccgc cttccgaatc aaggggtacg aggcgaagag cccgacccag
      901 gggatcgcgc gcgtcggcct cgtcgacgtg ccccccgggc ggccggggcc cctcgaaggc
      961 gcgccgcgca ccctgacgct ggtgaaaaga gggtcgcgct ggctcgtgga cgactggcgc
     1021 tgacggaggc gcggcgagcg agggaggtta taatgcctct ccctcatccg gcgcggtgcg
     1081 gggaatctct tccatgaaga accacggctt caaagacgag tgcggtgtct tcgccgtctt
     1141 cggacacccg gaagcggcca acctcgtcta cctcgggttg cacgccctgc agcaccgcgg
     1201 ccaggagtcg gccggcatcg tctcgagcaa cggccagcgc ctcctcgagg agaaggggat
     1261 ggggcaggtc ggccgcgtct tcaagcgcaa gaacctgctg cgcctgaagg ggacccacgc
     1321 catcggccac gtgcgctact ccaccgccgg caagagcgcg ctcgtcaacg cccagccgtt
     1381 ccgcatcgac tcctgcaagg gggagatcgc catcgggcac aacggcaacc tggtcaacgc
     1441 cgaggccctg cggaagagcc tcgaggccgc cggatcgatt ttccggtcct cctccgacac
     1501 cgaggtcgtc ctgcacctga tcgccaaggc gccgcagagc gacctcgagg cggcgatcgt
     1561 ccacgccctc aagcaggtgg agggggcgta ctcgctcgtc ttcatcaccc ccgaccggac
     1621 catcggcgtg cgcgatccgc gcggcttccg cccgatggcg ctcggcaagg ccggggacgc
     1681 acacgtgctc acgagcgagt cgtgcgccct cgacctcatc gacggcgcct tcgtgcgcga
     1741 ggtcgagccg ggggagatgg tggtgatcag cgcccagggg gtgaagtcgt accggccatt
     1801 cccggccgcc ccgccgtcgc agtgcatctt cgagcacgtg tacttctcgc gccccgactc
     1861 gttcgtcttc gggcggagcg tgcacgaggt gcgcaaggcg ctgggacgcg agctggcgcg
     1921 cgagcagccg gtcgaggcgg acgtcgtcgt gccgatcccc gactcggggg tgtacgcggc
     1981 gctcggcttc gccgagggct cggggctgcc gctcgagttc ggcttcgtgc gcaaccacta
     2041 catcggccgc accttcatcg agccgcgcca gtcgatccgg cacttcggcg tcaagctgaa
     2101 gctcaacccg gtgaagcgcc tgctcgaggg gcggcgggtc gtgctcatcg acgactcgat
     2161 cgtgcgcggc acgacctcgc gcaagatcgt cgccatggtg cgtgccgccg gcgcccgcga
     2221 ggtccacatg cggatctcct gcgccccgac ggtcggcccc tgctactacg gcgtggacac
     2281 gccgcgccgc aaggacctca tcgccgccaa ccacacggtg gaggagatcc gccgcttcat
     2341 cgaggccgac tcgctcggct acctgtcgct cgaggggatg gcccgcgccg tcggcggtgc
     2401 gaaggggttc tgcacctcct gctacaccaa cgagtacccg gtcccgatcc cggacgaggc
     2461 ggcggtgcgg gtcgccgcgc cgccccggct ccgctgagga acagcgcacc catgaccgat
     2521 ccgttcgcca cctgggactc cgaccgcctc gccatcacca cgctcaagat gctggcggtc
     2581 gacgccgtcg aaaaagcgaa gtccggacac cccggcatgc cgatggggat ggcggaggtc
     2641 gcgtacatgc tgtggacgaa gcacctgcgc ttcgatccgg agcgctccga ctggcaggac
     2701 cgggaccgtt tcgtcctgtc ggccggccac ggctcgatgc tcctgtacgg cctcctgcac
     2761 ctctcgggct acgaccttcc gctcgaggag ctgaagcgct tccggcagtg ggagagcaag
     2821 acgcccgggc accccgagta ccgccacgct cccggcgtcg agacgacgac cggcccgctc
     2881 gggcagggtc tcggcaacag cgtcgggatg gcgatcggcg ccaagcgcct ggcggcccgc
     2941 ttcaacacct ccgaccgccg catcatcgat caccgcgtct gggcgctggt cagcgacggc
     3001 gacatgatgg agggggtcgg gtcggaggcg gcgtcggtcg ccgggcacct caagctcggc
     3061 aacctgacgc tcctctacga cgacaaccac atcaccatcg agggctcgac cgacctcgcc
     3121 ttctcggagg acgtcggcaa gcgcttcgag gcgtacggct tccgcgtctg gcgcatcgac
     3181 ggccacgatc tcgccgccat cggccggacg ctcgacgagg ccgcgaagga gcgcgcccgg
     3241 cccgggatcg tcatctgccg gacgcacatc ggccacggcg cgccgcacaa gcaggacacc
     3301 gccgaggcgc acggctcgcc gctcggcccc ga
//
LOCUS       DASRIQ010000359         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2475207, whole genome shotgun sequence.
ACCESSION   DASRIQ010000359 DASRIQ010000000
VERSION     DASRIQ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2475207"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFD06_08575"
     CDS             complement(<1..324)
                     /locus_tag="VFD06_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HZN03631.1"
                     /translation="MTRYIVGITGASGSLCARAILNALLDHRRVGEVHVVLSAYSLRT
                     LHAELGVEVRDEAAGLRALLGRRRSRAGGARLVLHRSDAMEASISSGSFRTDGMLVVP
                     CSGGTL"
     gene            complement(321..671)
                     /locus_tag="VFD06_08580"
     CDS             complement(321..671)
                     /locus_tag="VFD06_08580"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZN03632.1"
                     /translation="MTQANEKRRHPRVSEQLTIRAAHDGGVAFQTINLSAGGMFCTSP
                     TFIAPMTRLSLMMEVPHSDAPARIEGEAVVVRTEPEAPVPSHRDGYRIALFFSRLADD
                     DRRTLQAYLSSRGQ"
     gene            809..1567
                     /gene="larB"
                     /locus_tag="VFD06_08585"
     CDS             809..1567
                     /gene="larB"
                     /locus_tag="VFD06_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZN03633.1"
                     /translation="MQTRSLRGLLRRIHRGGISIDAALERLRALPFEDLGFAKIDTHR
                     ELRRGFPEVIFGEGKTVPQIVAIVDRIAARGQPVLVTRVGEEVHRRVRERHPGARYHA
                     AARAVTVGVGRRRGRGRPGILVLAAGTSDLPVAEEAVLTAEVMGNRVERAYDVGIAGL
                     HRLLAQRGKLRKARVIVAVAGMEGALPSVVAGLVAVPVIGVPTSIGYGTGLKGVAALM
                     GMLNSCSTGLMVVNIDNGFGAGYSASIINSMSNK"
     gene            1876..2082
                     /gene="rpsU"
                     /locus_tag="VFD06_08590"
     CDS             1876..2082
                     /gene="rpsU"
                     /locus_tag="VFD06_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZN03634.1"
                     /translation="MPLVRVREDESLENALRRFKRKCEKSGVLSELKKRQHYLKPSQK
                     RKIKALAARKKALKRLAQERKFND"
     gene            2165..>3351
                     /gene="dnaG"
                     /locus_tag="VFD06_08595"
     CDS             2165..>3351
                     /gene="dnaG"
                     /locus_tag="VFD06_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZN03635.1"
                     /translation="MRAAADLFEVVSEYVALKKSGARWRGLCPFHQEKTPSFYVDPDK
                     QLYYCFGCSSGGDVFKFLMLYEKLEFPEVLRSLASRYNVTLPEARGGTEVSEKQRLVG
                     LNRQAMGFFHDQLLRPSGERARRYLGSRGIRREVAERFGLGYAPEGWTALKDHLGRAG
                     GGAEALALAAGLLGKKEETGRTYDRFRDRVIFPIVGLTGDVLGFGGRVLGEGEPKYLN
                     SPDSAAFNKGENLYALPFAKDAIRKSGFAILVEGYMDAIALQTAGVEQAVATLGTGFT
                     PGHVRLLKRFTESVVVNFDPDAAGRQAARRSIETLLENGFEVKVVALPPGKDPDRYVR
                     DEGVERYRERVNGALPYVEYLAREVAGRVDVSATQGKVKALNEVLPFLARMDNPVRRA
                     AQV"
BASE COUNT          550 a         1018 c         1237 g          546 t
ORIGIN      
        1 gagcgtcccg ccgctgcacg gcacgaccag catgccgtcg gtccggaacg acccgctgct
       61 gatcgacgcc tccatcgcgt cggagcggtg cagcacgagc cgggctccgc cggcgcggga
      121 ccggcgccgg ccgaggagcg cccgaagccc cgccgcttcg tcgcgcacct cgaccccgag
      181 ctcggcgtgg agcgttcgga gcgagtaggc cgacaggacg acgtgcactt cgccgacccg
      241 ccggtgatcg agaagcgcgt tcaggatggc ccgggcgcag agcgatccgc tcgcgccggt
      301 gatcccgacg atgtaccgcg tcactgcccc cggctgctga gatacgcctg cagggtgcgc
      361 cggtcatcgt ccgccaggcg ggagaagaac agcgcgatgc ggtagccgtc gcggtgcgaa
      421 gggaccggag cctccggctc ggtgcgcacc acgaccgcct ccccctcgat gcgggcgggc
      481 gcgtcgctgt gcgggacctc catcatcagc gacagccggg tcatcggcgc gatgaaggtc
      541 ggcgaggtgc agaacatccc gccggcgctg aggttgatcg tctggaaggc gacgccgccg
      601 tcgtgcgcgg cgcggatggt cagttgctcg gacacacgcg ggtggcgacg cttctcgttg
      661 gcctgggtca tggagtcctc ggcgcgaccg cggggcggtg gcgcgtgatg ggtggcgaca
      721 gagccggaac ggtccgcgat ggtaggaaag ggcggaacct ctgtcaagga gcgcctataa
      781 tccggcgctc cggagccaca tcgatcccat gcagactcgt tccctgcgcg gcttgcttcg
      841 ccgcatccac cgcggcggca tctcgatcga cgcggccctc gagcgcctgc gggccctgcc
      901 gttcgaggac ctcggcttcg ccaagatcga cacccaccgg gagctgcgcc gcggctttcc
      961 ggaggtgatc ttcggcgagg gaaagaccgt gccgcagatc gtcgcgatcg tcgatcggat
     1021 cgcggcgcgc ggccagccgg tgctggtgac ccgggtgggg gaggaggtgc accggcgcgt
     1081 gcgggagcgc catcccggcg cccgctacca cgcggccgcc agggccgtga cggtgggggt
     1141 cggccgccgc cgcggacggg ggcgtccggg gatcctggtg ctggcggccg gcacctccga
     1201 cctgccggtg gcggaggagg cggtgctgac cgccgaggtg atggggaacc gggtcgaaag
     1261 ggcctatgac gtcgggatcg cgggtctcca ccgcctgctg gcgcagcggg gaaagcttcg
     1321 gaaggcgcgg gtcatcgtgg cggtcgcggg gatggagggg gcccttccga gcgtcgtcgc
     1381 ggggctcgtg gccgtgccgg tgatcggggt cccgacgagc atcggctacg gcaccggact
     1441 caagggcgtc gccgccctca tggggatgct caactcctgc tcgacagggc tcatggttgt
     1501 gaatatcgac aacgggttcg gggctggata ttccgcatcg ataatcaaca gtatgagtaa
     1561 taagtagtta tgagttattt agctccgttt ggcacggagt catgagtggt aacgtccata
     1621 aaaggtacgc gcgcatcccg ttttttgata tcatccgtcc cttccttgac atctcaccaa
     1681 ccggcccgta tattacgaac gcatcggtct gttggtcatt ggtaattcct tgttgagcca
     1741 cgtcttacct ccgctcaacg cgtttttatc agagatcgca ccccggtcgt tcgggctcct
     1801 caagagagca gggatttctc tcgggagggg cggaaggacc ggtgtctggg cacacatgaa
     1861 gggagggatt accggatgcc cctggtcagg gtgcgcgagg acgagtcgct ggagaacgct
     1921 cttcgccgct tcaagagaaa gtgcgagaag tcgggcgttc tctcggagct caagaagcgc
     1981 cagcactacc tgaagccgag ccagaagaga aagatcaagg ctttggctgc gcgcaagaag
     2041 gccctcaagc gtctggcaca ggaacggaaa ttcaacgact gagagaacgg cctctcggtt
     2101 cgttggcggg caggcacagg agcgcagggt ggggacgggg aatcgatccg attccgtcga
     2161 actcgtgaga gcggcggccg atctgttcga ggtcgtctcg gaatacgtcg ccctcaagaa
     2221 gagcggcgcc cgctggcgcg gcctctgtcc gtttcatcag gaaaagacgc cgtcgtttta
     2281 cgtggatccg gacaagcagt tgtactactg cttcggctgc tcgtccggag gggatgtctt
     2341 caagtttctc atgctgtacg agaagctcga gttccccgaa gtgcttcgtt ctttggcatc
     2401 ccggtacaac gtcacgctcc ccgaggcccg cggcggcacg gaggtcagcg agaagcagcg
     2461 cctggtcggg ctcaaccggc aggcgatggg cttctttcat gaccagctgc tgcggccgtc
     2521 gggcgagcga gcgcgccggt acctcggatc gagggggatc cggcgcgagg tcgcggagcg
     2581 gttcggcctc ggctacgcgc cggagggctg gactgctctc aaggatcacc tcgggcgagc
     2641 gggcggcggc gcggaagcgc tggcgcttgc cgccgggctc ctcgggaaga aggaggagac
     2701 ggggcgcacc tacgatcgct ttcgcgaccg ggtcatcttt ccgatcgtcg ggctgaccgg
     2761 tgacgttctc ggattcggcg gacgcgtgct cggggagggg gagccgaaat acctgaactc
     2821 tcccgactcg gcggcgttca acaagggcga gaacctgtac gcgctgccgt tcgcgaagga
     2881 cgcgatccgg aaatcgggct tcgcgatcct ggtcgagggt tacatggacg cgatcgcgct
     2941 gcagacggcg ggagtggagc aggcggtggc gacgctcggg accgggttca ctcccgggca
     3001 cgtgcggctc ctgaagcggt tcaccgagtc ggtggtggtg aatttcgacc ccgacgcggc
     3061 cggacggcag gcggcgcggc ggtcgatcga gacgctgctg gagaacggct tcgaggtgaa
     3121 ggtcgtggcg ctgccgccgg ggaaggaccc ggaccgctac gtccgggacg aaggagtgga
     3181 gcgctaccgc gagcgggtga acggggcgct gccgtacgtg gaatacctgg cccgcgaggt
     3241 ggccggccgg gtggatgtga gcgccacgca gggaaaggtg aaggcgctca acgaggtgct
     3301 gccgttcctg gcgcgcatgg acaacccggt gcgccgggcg gcgcaggtgg a
//
LOCUS       DASRIQ010000360         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2216986, whole genome shotgun sequence.
ACCESSION   DASRIQ010000360 DASRIQ010000000
VERSION     DASRIQ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216986"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFD06_08600"
     CDS             <1..1002
                     /locus_tag="VFD06_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HZN03636.1"
                     /translation="YGGMENPRMTFLTPTLLAGDRSLVDVVAHELAHSWTGNLVTNAS
                     NEHFWLNEGTTVYAERRIQEALHGAEAAALSWAIGAKGLHADFERFASRPELTRLRLD
                     LEGVDPDDAFSSVPYEKGARLWALLERTAGRERFDAVLRRYIESFRFTSITTEDWLRF
                     IEKELPGIGEAVRIHEWLHEPGLPANAPRFEAPALDRLTALASGWRDGARPAPDQIAA
                     WGTSETLVYLQRLPRPLQAAECAWLDRAFALDGRGNYEILVEWLTIAAASAYEPAFPR
                     LRDVLTRVGRMKYLRPLYTALGASSETQALGRDIFAGARPGYHPLSRRAVEAILEKY"
     gene            1115..1474
                     /locus_tag="VFD06_08605"
     CDS             1115..1474
                     /locus_tag="VFD06_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03637.1"
                     /translation="MKGLRILVVLAVAALLAAPAANTFAGDQDKPTAAEPGKKATTDK
                     QVTVVSVDPSAKTLTFKGPDGKEKTVPVTGQAVNKLKEVRPGQQITVTCTSDDAGEIA
                     SISDLKLEPAAGTTRQP"
     gene            1587..3008
                     /locus_tag="VFD06_08610"
     CDS             1587..3008
                     /locus_tag="VFD06_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HZN03638.1"
                     /translation="MRGVLGIAAFLACAAPTAEGTGAAAAEDDPAPGDDAASAAAAPD
                     AEALRQRIQALEEELALLRREAALTEDIQKRLTALEAELNRLREERPPAGGATITLRS
                     VGGLGEAASRVYLTKRGLAFGGYGSALYQRFTQTRDDDTDANVPDRADLTEAFLYVGY
                     RFDDRLLFNSSFGVEHALVEEGSAGSATVEFAYVDFRQRKEIGWRGGLLLMPVGWLNE
                     RHEPNDYLGTTRPITEQRILPSTWRGVGAGAYGEAGPVEWRGYVIESLDAAGFTALEG
                     IAGGRQQGSESLVEDMGLTARVDWRPIRDRGLGELVLGASGFTGRTGQEQPGFPDGRL
                     SLWDAHAEYRWRGFQARALYASGILGGAGEISLAIDPSGLTAIGERMEALYAEVGYDV
                     LSTWPRSKQDLIVFCRFENLDTQAEVASGLTTDPLADLTVKTCGIQYAPVRQVVIKAD
                     ATNYVDPGQQSVDQVNLGLGWVF"
BASE COUNT          485 a         1012 c         1099 g          451 t
ORIGIN      
        1 tatggaggga tggagaaccc ccgcatgacc ttcctgacgc cgaccctcct ggcgggggac
       61 cgatcgctcg tggacgtcgt ggcgcacgag ctggcccact cctggaccgg caacctcgtc
      121 accaacgcca gcaacgaaca cttctggctc aacgagggga cgacggtgta cgcggagcgc
      181 cgcatccagg aggcgctcca cggcgccgag gcggcggcgc tgtcgtgggc catcggcgcg
      241 aaggggcttc acgcggattt cgaaaggttc gcgtcgcgtc ccgagctgac gcggctgcgg
      301 ctcgacctcg agggcgtcga ccccgacgac gcgttctcct cggtcccgta cgagaagggg
      361 gcgcggctgt gggcgctcct cgagcgcacg gcggggcgcg agcgcttcga cgcggtcctg
      421 cgccggtaca tcgagtcctt ccgtttcacc tcgatcacga cggaggactg gctgcggttc
      481 atcgagaagg agctaccggg gatcggagag gcggtccgga tccacgagtg gctgcatgag
      541 cccggactgc cggccaacgc cccgcgcttc gaggcgccgg cgctcgaccg gctcacggcg
      601 ctggcgtcgg gatggcgcga cggcgcgcgc ccggcgccgg accagatcgc cgcgtgggga
      661 acgagcgaga cgctcgtcta tctccagcgc ctgccgcgac cgctccaggc tgcggaatgc
      721 gcctggctcg accgggcgtt cgcgctcgac ggccgcggca actacgagat cctggtcgag
      781 tggctgacga tcgccgccgc gtccgcgtac gagccggcgt tcccgcgtct gcgcgacgtg
      841 ctgacgcgcg tcggccggat gaaatacctg cgtccgctct acaccgccct cggcgcatcg
      901 tccgaaacgc aggcgctggg gcgcgacatc ttcgccggcg cgcgtccggg ctaccatccg
      961 ctgtcgcgaa gagccgtaga agcgattctg gaaaaatact gagacacatt catttgatcg
     1021 tcgcgtcaga atcgctgtgc tagcatggct cttgcttgca gggtgacgac tttgtgaatg
     1081 ccccctccca atatcatcaa ggaggcgttc catcatgaag ggacttcgaa ttctcgtggt
     1141 tctggccgtg gcggcactcc tggctgctcc ggcggcgaac acgttcgccg gcgatcagga
     1201 caaaccgacc gcggcggagc ccggaaagaa ggccaccacg gacaagcagg tgacggtagt
     1261 gtccgtcgat ccgagcgcga agacgttgac gttcaaggga cccgacggca aggagaagac
     1321 cgtcccggtg accggccagg cagtcaacaa gctgaaggag gttcggccgg gacagcagat
     1381 caccgtgacc tgcaccagcg acgacgcggg cgagatcgct tcgatctccg acctgaagct
     1441 cgagccggcg gccggcacga cccggcagcc gtaaggcagg caccgcattg cggtaaggcg
     1501 gggcgttcgg cggtgactcc gaacgccccg ctttttttgt cctgacgggg gaatcggtac
     1561 aaatgcggac aatgcggatc gtgtcattga gaggggtgtt ggggatcgcc gcgtttctcg
     1621 cgtgcgccgc accgacggcg gaagggaccg gggccgcggc cgccgaagac gatccggcgc
     1681 ccggggacga cgccgcttcc gccgcggcgg ctccggacgc cgaggcgctg cgacagcgca
     1741 tccaggcgct cgaggaggag ctcgcgctcc tgcgacgcga ggcggccctc acggaggaca
     1801 tccagaagcg gctgaccgcc ctcgaggcgg agctgaaccg cctgcgcgag gagcgtccgc
     1861 cggccggcgg agcgaccatc acgcttcgga gcgtgggcgg gctgggagag gcggcgtcgc
     1921 gcgtctacct gacgaagcgc gggctggcgt tcgggggcta tgggtccgcg ctgtatcagc
     1981 gattcacgca gacgcgcgac gacgacaccg acgccaacgt cccggatcgc gccgatctga
     2041 ccgaggcctt tctctacgtg ggctaccgct tcgacgaccg gctgctgttc aactcctcgt
     2101 tcggcgtcga gcacgcgctc gtcgaggaag gatcggcggg gagcgccacg gtcgagttcg
     2161 cctacgtcga cttccggcag cgcaaggaga tcggctggcg cggcggcctg ctgctgatgc
     2221 ctgtcggctg gctcaacgag cgccacgagc cgaacgacta cctcggcacg acgcgcccga
     2281 tcaccgagca acgcatcctt ccttccacct ggcgcggagt cggcgcggga gcgtacggag
     2341 aagccggacc ggtcgagtgg cgcgggtacg tcatcgagtc gctcgacgct gccggcttca
     2401 ccgcgctcga ggggattgcg ggcggccggc agcagggctc cgagtcgctc gtcgaggaca
     2461 tgggactgac ggcgcgcgtc gactggcggc cgatccgcga ccgcgggctc ggcgagctgg
     2521 ttctcggcgc ctcgggattc accggccgga ccggccagga gcagcccggc ttcccggacg
     2581 ggcggctgtc gctctgggac gcgcatgccg agtaccgctg gcgcggcttc caggcgcgcg
     2641 cgctgtacgc ctcggggatc ctcggcggcg cgggggagat cagcctggcg atcgatccgt
     2701 cggggctgac ggcaatcggc gagaggatgg aggcgctcta cgcggaggtc ggctatgacg
     2761 tcctgagcac ctggccccgg tcgaagcagg atctcatcgt cttctgccgc ttcgagaacc
     2821 tcgacacgca ggccgaggtg gcgtcggggc tgacgaccga tccgctggcg gacctgaccg
     2881 tgaagacctg cggcatccag tacgccccgg tccggcaggt ggtgatcaag gcggacgcga
     2941 ccaactatgt cgacccggga cagcagtcgg tcgatcaggt caacctgggt ctcggctggg
     3001 tgttctgacg tccgcgcgcc ggtcgcaccg gcgccggcgc ttcaggc
//
LOCUS       DASRIQ010000361         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3536148, whole genome shotgun sequence.
ACCESSION   DASRIQ010000361 DASRIQ010000000
VERSION     DASRIQ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3536148"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFD06_08615"
     CDS             <1..429
                     /locus_tag="VFD06_08615"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HZN03639.1"
                     /translation="LRWVLGRQATDRNPFLWQLLLGRFAPGAAHAWLTAPSPAAMPAL
                     RWTVGTMDEALERHSGAFDFVHLSNILDWLSPDAAARTLRLASNTLATGGWVFVRQLN
                     SSLDIRSLGPAFRWEAEPSEQLLQEDRSFFYRSLHLGRKP"
     gene            426..1373
                     /locus_tag="VFD06_08620"
     CDS             426..1373
                     /locus_tag="VFD06_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZN03640.1"
                     /translation="MTITLLDRAPSSDLAAGLADFEAGFTYPLGAGRGFRISHGSDYS
                     RFFRSMGRAACFVAQDGNRVVGALACVVRHLSLPAGGACEAAYLADLKVLPGAGSGRI
                     LLRLASAAQTWGGRFTDKAFAVVMEGTRATPDRYTGRVGIPRLEEVARIAILRIPTDG
                     SAGAPVGRYVDDPDRAEISWRALAAGACYASGGDPAERSEISPEPIALADGAACGRLE
                     DTRRAKRLITLEGDEMISAHLARFAFRSPEAGADLIREACRRSGARGYPALFVAVGDS
                     AAAAIHERLADCSITVAPARVYACALSTGSPWIVNTSEI"
     gene            1376..2089
                     /locus_tag="VFD06_08625"
     CDS             1376..2089
                     /locus_tag="VFD06_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HZN03641.1"
                     /translation="MHDQGRRSSRAAAVAARAGEILDRDGLLRNPYLTALSAGTMSRE
                     AFRLTQEQFFFAVTFFPRPMAALVGRIEDPAARLDILHNLVEEHGEFDPRAFHHNTFQ
                     EFLSRLGSDVSSLQGTSVWPELRAFNSVLIAACVLDELEVGVGCMGIIEHAFAGISAA
                     IGQAVVDRGWLTEDRLVHYRLHAEIDIRHSEEFYAVVEPLWSDEARRYYIVQGLELGA
                     YAFRRLYTDLSERARGMAD"
     gene            complement(2086..3177)
                     /locus_tag="VFD06_08630"
     CDS             complement(2086..3177)
                     /locus_tag="VFD06_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03642.1"
                     /translation="MKTSTAPRLSQSERRALRRQSERPPRLLRGLQIQLDRFRVWSKI
                     TWHRGRVLATTGDSWKSHDAPEYLRDPELFYRGERKVPVVTTSPVRRRIAGGEVERFT
                     FPTLHRMPIPETNTAFGRFYGVRGVPDAPVVLISHGWAHRMLRTIEHLYVRPFLKAGF
                     AVAFVSHPMHLERTPRGSYSGELVVSADVVLTVDAFRQGVIDLIGAADWLRSRGHTQV
                     GVLGYSLGGYLAGIMAAVRDDWSFVVIGGAGHSPVSPILDTPLGTNVREDLAACGMGD
                     RASLTRAWTIISPGAFQPKVSKERILLVAGLYDQIMLPRSVRRLWNVWDRPALHWLRR
                     SHYTLLALNGGLLSHAIPFMRDSTGRRTD"
     gene            complement(3178..4059)
                     /locus_tag="VFD06_08635"
     CDS             complement(3178..4059)
                     /locus_tag="VFD06_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN03643.1"
                     /translation="MSDVRGGPLRIDRHPRLYRDNRYVYPVLSRRAHGISIGVNLNPD
                     KICNFDCIYCQVDRVTPPVVRDVDEARLYDELRDTLRQAKSGALFEREEFRALAPAER
                     EIRDVTFAGDGEPPSYPNFSGVVRETIRIKKEEGFAALPINLLTNATLIDRPRVQQGM
                     RLIDADGGAHWLKLDAGSEEYYRLVERTTIPFARVLANITQAARERPVVIQSLFMRIQ
                     GEGPSRGEIDAWCGRLASIVADSGRIRLVQVYTVARPPAESYVTPLSDGDVDAIADEA
                     RRRLPGVVVESFYSGRF"
BASE COUNT          622 a         1504 c         1440 g          664 t
ORIGIN      
        1 ctgcgctggg tcctgggccg tcaggcgacg gatcgcaatc cgttcctgtg gcagctgctg
       61 ctgggacgat tcgccccggg tgccgctcac gcgtggctca cggcaccgag cccggcggcc
      121 atgccggcgc tgcgatggac agtcggcacg atggacgagg cgctggaacg acattccggc
      181 gcattcgact tcgtccatct ttcgaatatc ctcgactggc tgtcgcccga cgcggcggcc
      241 cggacgctcc gcctggcatc gaacacgctc gccacgggag gatgggtctt cgtccgccag
      301 ctgaactcat ccctcgacat acgctctctg ggcccggcct tccgatggga ggccgaaccg
      361 tccgaacagc tcctccagga ggatcgaagc ttcttttatc gatcgctgca cctcggcaga
      421 aagccatgac catcaccctt ctcgatcgcg caccctcgtc cgatctggcc gcgggcctcg
      481 cggatttcga ggccgggttc acctatccgc tgggagcggg acggggattc cggatcagcc
      541 atgggtcgga ctattcgcgg ttcttccgtt ccatgggacg cgctgcctgc ttcgtcgccc
      601 aggacgggaa ccgcgtcgtc ggcgcactgg cgtgcgtggt ccggcacctg agcctccccg
      661 cgggaggcgc atgcgaggcg gcttatctcg ccgacctcaa ggtgctcccg ggcgccggtt
      721 ccggcaggat tctgctgcga ctcgcgtccg cggctcagac ctggggcggc cgtttcacgg
      781 acaaggcgtt tgccgtggtc atggagggga cgcgtgcgac ccccgatcgc tacaccggcc
      841 gcgtcggcat cccccgcttg gaggaggtgg cccgcatcgc gatcctgcgc atcccgacgg
      901 acggctcggc cggcgctccc gtcggccgat acgtggacga tcccgatcgc gccgagatct
      961 cctggcgagc tctcgcggcc ggggcatgtt acgcctcggg aggcgacccg gcggagcgct
     1021 ccgagatatc tccggagccg atcgccctgg ccgacggcgc cgcgtgcggc cggctggagg
     1081 atacccgccg ggccaagcgc ctgatcacgc tcgaagggga cgagatgatc agcgcccacc
     1141 ttgcccgctt cgcgttccgg tctcccgaag ccggcgcgga tctcatccgc gaagcctgcc
     1201 ggcgatccgg ggcgcgcggc tatccggccc tgttcgtggc cgtcggcgac agcgcggcgg
     1261 ccgccatcca cgagcgcctc gcagattgct ccatcacggt cgccccggcc cgggtgtacg
     1321 cttgcgcgct cagcaccgga agcccgtgga tcgtcaacac ctcggagatc taggcatgca
     1381 cgatcaggga aggcgctcgt cgcgggcggc ggccgtggcg gcgcgcgccg gcgagatcct
     1441 ggaccgggac ggcctcctcc ggaatcctta tctgacggcc ctgtcggcgg ggacgatgtc
     1501 ccgcgaggcg ttccgtctca cccaggagca gttcttcttc gcggtgacgt tcttcccccg
     1561 gccgatggcg gcgctcgtcg ggcgcatcga ggaccccgcg gcccgtctcg acattctcca
     1621 caacctggtc gaggagcacg gtgaattcga cccgcgcgcg ttccaccaca acaccttcca
     1681 ggagttcctg agccgcctgg ggagcgacgt ctcctcgctt cagggaacgt cggtctggcc
     1741 ggagctgcgc gccttcaaca gcgtgttgat cgccgcctgc gtactcgacg agctggaagt
     1801 cggggtcggc tgcatgggga tcatcgagca cgcgttcgca ggaatctccg cggccatcgg
     1861 acaggccgtc gtcgatcgcg ggtggctcac cgaggatcgc ctcgtccact accggctgca
     1921 cgcggagatc gacatccgtc attccgagga gttctacgcg gtcgtggagc ccctgtggtc
     1981 cgatgaagcg cggcgctact acatcgttca gggactcgag ctgggcgcct acgccttccg
     2041 tcgtctctac accgacctct ccgaacgcgc acgcggaatg gccgattagt cggtgcgacg
     2101 gcccgtgctg tcgcgcatga acgggatggc atggctcagg agcccgccgt tcagcgccag
     2161 cagggtgtag tggctgcgcc gcagccagtg cagcgccggc cggtcccaga cattccagag
     2221 acggcgcacc gagcgcggca gcatgatctg gtcgtacaac cccgcgacga gcaggattct
     2281 ctctttcgag accttcggct ggaacgcgcc gggtgagatg atcgtccagg cgcgcgtcag
     2341 cgacgcccgg tcccccatcc cgcaggccgc caggtcctcg cggacgttgg tgccgagggg
     2401 ggtgtcgagg atcggcgaca ccggcgagtg cccggctccg ccgatgacga cgaacgacca
     2461 gtcgtcgcgc accgccgcca tgatgccggc gaggtacccg ccgagggagt agccgaggac
     2521 tccgacctgt gtgtgcccgc ggctcctcag ccagtcggcg gcgccgatga gatcgatcac
     2581 cccctgccgg aaggcgtcca cggtgagcac cacgtcggcg ctgaccacca gctcgccgct
     2641 gtacgagccg cgcggggtgc gctcgaggtg catcgggtgg gagacgaacg ccaccgcgaa
     2701 gccggccttg aggaacgggc gaacgtagag gtgctcgatg gtcctcagca tgcgatgcgc
     2761 ccagccgtgg ctgatgagga cgaccggcgc gtccggcacg ccgcgcacgc cgtagaagcg
     2821 gccgaaggcg gtgttggtct ccgggatcgg catccggtgc agcgtcggga aggtgaaccg
     2881 ctccacctcg ccgccggcga tgcggcggcg gaccggcgag gtcgtgacca ccggcacctt
     2941 gcgctcgccc cggtagaaca gttccggatc gcgcaggtac tccggcgcgt cgtgactctt
     3001 ccaggaatcc cccgtcgtcg cgaggacgcg gccgcgatgc caggtgatct tcgaccagac
     3061 gcggaagcga tcgagctgga tctgcagacc gcgcagcagc cggggcggac gctcggactg
     3121 ccggcgcagc gcccgccgct cgctctgact caaccggggc gcggtggagg tcttcaacta
     3181 gaaccggccg ctgtagaacg attcgaccac gacaccgggg aggcggcgcc gcgcttcgtc
     3241 ggcgatggcg tccacgtcac cgtccgaaag cggcgtgacg tacgactccg ccggcggccg
     3301 cgccacggtg tacacctgca ccaggcggat ccggccagag tcagccacga tcgacgcgag
     3361 cctcccgcac caggcgtcga tctccccgcg cgacggccct tccccctgga tgcgcatgaa
     3421 gaggctctgg atgaccaccg gccgctcccg cgccgcctgg gtgatgttgg cgagcacgcg
     3481 cgcgaatggg atcgtcgtcc gctcgaccag ccggtagtac tcctcgctgc cggcgtcgag
     3541 cttcagccag tgggcgcctc cgtccgcgtc gatgagccgc atgccttgct gcacgcgcgg
     3601 ccggtcgatg agcgtggcgt tggtgagcag gttgatcggc agcgccgcga acccctcttc
     3661 cttcttgatc cggatcgtct cccgcaccac gccggagaaa ttcgggtagg aaggcggctc
     3721 gccgtcgccg gcgaaggtga cgtcgcggat ctcacgctcc gccggagcga gggcgcggaa
     3781 ctcctcccgc tcgaagagcg cgccgctctt cgcctgtcgc agcgtgtcgc gcaactcgtc
     3841 gtagagacgc gcctcgtcca cgtcgcggac gaccggaggg gtgacgcgat ccacctggca
     3901 gtagatgcaa tcgaagttgc agatcttgtc cgggttcagg ttgacgccga tcgagatgcc
     3961 gtgcgcccgc cgcgacagca ccggatagac gtaccgattg tcgcgataga gacgcggatg
     4021 gcgatcgatc cgcagcgggc ccccccggac gtcggacacg cgctcattat aatcggccgc
     4081 gtgcgccgcc cccgcaggtc cgtttcctcc ccgatccgct ccgaagccgg gaccgccgcc
     4141 gcgctcgtgg cctggttccg ccggcaccgg cgcgacctcc cctggcggca cgccccgccg
     4201 ccgctgcaag cgggccgccg ccgcgacccc
//
LOCUS       DASRIQ010000362         3990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3536976, whole genome shotgun sequence.
ACCESSION   DASRIQ010000362 DASRIQ010000000
VERSION     DASRIQ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3536976"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1253
                     /gene="rho"
                     /locus_tag="VFD06_08640"
     CDS             60..1253
                     /gene="rho"
                     /locus_tag="VFD06_08640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZN03644.1"
                     /translation="MLPDGGGFLRQPARNYRPSPEDVFVGREILQPFTPAVPPTNGTG
                     IEGIEIDGLAVATGRGGVSHEEPRHGGRHGQGGRHGQDRRGGGGRQAAARLTEIRTIN
                     GRTPADFAERRAFSTLTSIDPFEHIKLETEPGEISTRVIDILTPIGKGQRCLIVAPPR
                     AGKTTLLQKIAAAISKNHPEIDLMVLLVDERPEEVTDMKRNVRGEVIASSSDEMARQH
                     VSIARLVLERARRRVEVGGDVVILLDSLTRLARAHNAATPGTGRTLSGGLDARTMEQP
                     RRLFGAARKAEEGGSLTVIATALIDTGSRMDDVIFQEFKGTGNMELVLDRALFEQRIY
                     PAVDIGKSGTRKEEKLFSKEDLPRIHRLRRVLFEMKPHDAMSKLIDKLSKFPSNAAFL
                     KAIEV"
     gene            complement(1340..1699)
                     /locus_tag="VFD06_08645"
     CDS             complement(1340..1699)
                     /locus_tag="VFD06_08645"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZN03645.1"
                     /translation="MTAGAEKRLHHRFMARLDVRVVSGERVTPGEALTTLDIGVGGAR
                     CIAATKFEAGTRLQVSVQLVGGDLPEPAQVTAAAAVLRCEIRPEPHYGLPYEVVLQFL
                     RLDPRDRRMLQGYLNSL"
     gene            complement(1871..3688)
                     /locus_tag="VFD06_08650"
     CDS             complement(1871..3688)
                     /locus_tag="VFD06_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN03646.1"
                     /translation="MSWKRWLLGSPLETSRLAEERLTVALALAVFASDALSSVAYATE
                     EILLVLSTAGLAALNYSLPIALIITGLLALLIASYRQTVLAYPEGGGSYLVARENLGT
                     MPGLVSAAALLIDYVLTVAVSSAAGVAAVTSALPALAGHKVAMGVLFVSIIAVANLRG
                     VRESGRIFAIPTYTFIGSLVLLLAVGMWRATRGLPAIEAPVGVLQDATHAVTAFLLMR
                     AFSSGCAALTGIEAISNGVKAFRPPETRNAATTQVIMGCILGTFFLGITWLAHVYAIV
                     PGEGQTVISQLGRAVFGSGGLYFLLQAATAAILLVAANTAFAGFPRLTSILANDRFLP
                     RQLATLGDRLVYSNGILILSAAAMLLLIVFQGDTHALIPLYAVGVFLAFTLSQAGMVR
                     RHLRVRDGAYRMHAALSAAGAVATGAALVVIAITKFTHGAWIVVVLVPALVVSFLRTH
                     RHYFYVRTQLSLIEMEPERPIRHTAIVPISAVNRATIYALRYAKSIAHEVEAVHVALD
                     PRRAEMIQQQWRTWGGGVPLRMLESNYRSLITPLVDHIDDLMQGRRRNEMVTVVLPEF
                     VTAHWWEGLFHNQSAFLIKGALLFRPGVVVTSVPFHFKR"
     gene            complement(3717..>3990)
                     /locus_tag="VFD06_08655"
     CDS             complement(3717..>3990)
                     /locus_tag="VFD06_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZN03647.1"
                     /translation="LCGLERRAAADYSFLAAVPTMVAATLFDLFQSRGALDAGDAAPF
                     AVGFAVSFLAAWAAVRFFIALLGRTTLRPFAWYRIAIAPAILAVRG"
BASE COUNT          668 a         1397 c         1428 g          497 t
ORIGIN      
        1 ggccctcgca cgcgccggtc gcggcggcgg ctccgttcga gacgcacggc gtcctcgaac
       61 tgctcccgga cggcggcgga ttcctgcggc agccggcgcg caactaccgc ccctcccccg
      121 aggacgtctt cgtcgggcgc gagatcctcc agccgttcac cccggccgtg ccgccgacca
      181 acggcaccgg catcgagggg atcgagatcg acgggctcgc cgtggccacc ggacgcggcg
      241 gtgtctccca cgaggagccg cggcacggcg gtcgtcacgg gcagggcggc cggcacggtc
      301 aggaccggcg cggcggagga ggacgacagg cggcggcgcg gctgacggag atccgcacca
      361 tcaacgggcg gaccccggcc gacttcgcgg agcggcgcgc cttctccacg ctgacctcca
      421 tcgacccgtt cgagcacatc aagctggaga cggaacccgg cgagatctcg acgcgcgtca
      481 tcgacatcct cacgccgatc ggcaaggggc agcggtgtct catcgtcgcc ccgccgcgcg
      541 ccggcaagac gacgctgctg cagaagatcg ccgcggcgat ttcgaagaac caccccgaga
      601 tcgacctcat ggtgctgctc gtggacgagc gccccgagga ggtcaccgac atgaagcgca
      661 acgttcgggg cgaggtgatc gcctcctcct cggacgagat ggcgcgccag cacgtgtcga
      721 tcgcccgcct ggtgctggag cgggcgcggc ggcgggtcga ggtgggcgga gacgtggtga
      781 tcctgctcga ttccctcacc cgcctggcgc gcgcccacaa cgccgccacg ccggggaccg
      841 gccgcaccct ctccggcggg ctcgacgcgc gcaccatgga gcagccgcgg cggctcttcg
      901 gcgccgcccg caaggcggag gaggggggaa gcctgacggt catcgcgacc gcgctcatcg
      961 acaccgggtc gcgcatggac gacgtcatct tccaggagtt caagggaacc gggaacatgg
     1021 agctggtgct cgaccgggcc ctcttcgagc agcggatcta tcccgccgtg gacatcggca
     1081 agtccggcac ccgcaaggag gagaagctct tctccaagga agacctgccg cgcatccacc
     1141 ggctgcgccg cgtgctcttc gagatgaagc cgcacgacgc gatgtcgaag ctcatcgaca
     1201 agctctccaa gttccccagc aacgccgcct tcctcaaggc catcgaagtc tgaccccgcg
     1261 gcgtcgaagg cgccagcggc agcgtcagca acctgggatc aacctgtcgt tcgagtcccg
     1321 cttctaggag ccggccggat cagaggctgt tgaggtatcc ctgcagcatg cggcgatcgc
     1381 gtggatcgag ccgcaggaac tgcaggacca cctcataggg caggccgtag tgaggctccg
     1441 ggcgtatctc gcaacgcagc accgccgccg cggcggtgac ctgcgccggc tccggcaggt
     1501 cgccgccgac gagctgcacc gaaacctgga gccgcgtccc ggcttcgaac ttcgtcgcgg
     1561 cgatgcatcg ggcgccgccg acgccgatgt cgagcgtcgt gagcgcctcc cccggcgtga
     1621 cgcgctcccc cgacacgacc cgcacgtcga ggcgcgccat gaagcgatgg tgcagccgct
     1681 tctccgcccc ggccgtcatg gaccggatcc tagaggggcg tacctcaaaa cgcaacgccc
     1741 cacgccccgt acgttggagt cacgccctcc catctccacc ccccagccag ggagctcgct
     1801 cccgcccgct tcgtctcacg accgggaacc ccggcgggct ccccgctctg tcccgcgcgg
     1861 ccagggcctt tcagcgcttg aagtggaacg gcacggaggt gacgacgacg cccgggcgga
     1921 acagcagcgc gcctttgatg aggaaggcgg actggttgtg gaacaggccc tcccaccagt
     1981 gggccgtgac gaactccggc aggacgacgg tgaccatctc gttgcgccgg cggccctgca
     2041 tcaggtcgtc gatgtgatcg acgagcggcg tgatgagcga gcggtagttg gactccagca
     2101 tgcgcagcgg cacgccgccc ccccacgtcc tccactgctg ctggatcatc tccgcccggc
     2161 gcgggtcgag cgcgacgtgc accgcctcga cctcgtgagc gatcgacttg gcgtatcgca
     2221 gcgcgtagat ggtggcacgg ttcaccgccg agatcgggac gatggcggtg tggcggatcg
     2281 gacgctccgg ctccatctcg atgagcgaca gctgcgtgcg cacgtagaag tagtgccggt
     2341 gcgtccggag gaacgagacg accagggccg ggaccagcac gacgacgatc caggccccgt
     2401 gcgtgaattt cgtgatggcg atgacgacga gcgcggcacc ggtcgccacg gccccggcgg
     2461 cgctcagggc cgcgtgcatg cggtacgcgc cgtcgcgcac cctcaggtgc cgccgcacca
     2521 tgccggcctg cgagagggtg aacgccagga acacgccgac ggcatagagg gggatcaggg
     2581 cgtgcgtgtc cccctggaac acgatgagca ggagcatcgc cgccgccgac aggatcagga
     2641 tgccgttcga gtagacgagc cggtcgccga gcgtggcgag ttgccgcggc aggaagcgat
     2701 cgttggccag gatcgaggtg aggcgcggga agccggcgaa cgccgtgttg gcggcgacca
     2761 gcaggatggc ggcggtcgcg gcctgcagca ggaaatacag cccgccgctc ccgaagacgg
     2821 cgcgcccgag ctgcgagatc accgtctgtc cctctcccgg gacgatcgcg tagacgtgcg
     2881 cgagccaggt gatcccgagg aagaacgtcc cgaggatgca gcccatgatc acctgggtgg
     2941 tggccgcgtt gcgggtctcc ggcggccgga acgccttgac gccgttgctg atcgcctcga
     3001 tgccggtcag ggccgcgcag ccggaggaga aggcccgcat gagcaggaac gccgtcaccg
     3061 cgtgcgtcgc atcctggagc acccccaccg gggcctcgat ggccggcagc ccccgcgtcg
     3121 cgcgccacat gccgaccgcc aggagcagca ccagcgagcc gatgaaggtg taggtgggga
     3181 tggcgaagat ccggccggac tcccgcaccc cgcgcaggtt cgcgacggcg atgatcgaga
     3241 cgaacagcac tcccatcgcg actttgtgcc cggcgagcgc cgggagggcg gaggttacgg
     3301 ccgcgacccc ggcggccgag gacacggcga cggtcaggac gtagtcgatg agcagcgccg
     3361 ccgccgacac gaggcccggc atggtgccga ggttctcccg cgccacgagg tacgacccgc
     3421 cgccctccgg gtaggcgagc accgtctggc ggtacgaggc gatgagcagc gccaggagcc
     3481 cggtgatgat gagcgcgatc ggcagggagt agttgagcgc cgcgagcccc gccgtgctca
     3541 gcaccagcag gatttcctcg gtggcgtagg cgaccgacga cagcgcgtcg gaggcgaaga
     3601 ccgcgagcgc cagcgcgacg gtgagacgct cctccgccag ccggctagtc tcgagcgggg
     3661 atccgaggag ccagcgcttc cagctcacct cggctgcctg cccgggagag gccggctcat
     3721 ccgcgcaccg cgaggatcgc gggggcgatc gcgatccggt accaggcgaa ggggcgcaga
     3781 gtggtgcggc cgaggagcgc gatgaagaag cgcaccgcgg cccaggcggc gaggaacgag
     3841 acggcgaagc cgacggcgaa gggggccgcg tccccggcgt cgagcgcccc gcggctctgg
     3901 aagaggtcga acagggtggc ggcgaccatc gtcggcaccg ccgcgaggaa ggagtaatcg
     3961 gccgcggccc gccgctccag cccgcacagg
//
LOCUS       DASRIQ010000363         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3265813, whole genome shotgun sequence.
ACCESSION   DASRIQ010000363 DASRIQ010000000
VERSION     DASRIQ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3265813"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFD06_08660"
     CDS             complement(<1..633)
                     /locus_tag="VFD06_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZN03648.1"
                     /translation="MKGLASGWDIGGVHLKQVRLLDTEAGTRLDWRLRPFEIWREPSR
                     LAGELARMEPAGPLAVTLTAELSDAFTDRSAGVRAILDAVAAARGDDTFSVLHAEGRL
                     VSSADARKTPLAVASANWIAPATVAARRLPEGLVVDVGSTTTDVVPFSGGRPKPEGRT
                     DRERLSSGELVYSGVLRTPPAALAETVPLSGGSCRTIPEHFTQMADAYLLL"
     gene            complement(649..1632)
                     /locus_tag="VFD06_08665"
     CDS             complement(649..1632)
                     /locus_tag="VFD06_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZN03649.1"
                     /translation="MRIVVYEHFSRPGARASRGAARTILRAGRAMRDAVSRDLASVPG
                     TRLVPLRPGPPRRVLDRALARADAALVIAPEDGGLLERLAGAPARAGVLSLGPGPRAV
                     RLGADKIATGRLLRAAGVPVPAAGRKRAGAIVFKPRRGCGAEGVVVVRRPEDRRRGAR
                     IAARAARGDGIVAQAFVAGTALSASYLVRAGGDGERVGDLLCLGLGCQHLSGRFRLAY
                     TGGEIPGRLPPQAAAMVEEAARAAVAALARAAGDLRGFVGVDLVLGEAGPVVIEINPR
                     LTSSYLGLSRMAGGRIARFMIGAARGERLPARLRSSGRWRFHASGPVRRLA"
     gene            complement(1653..2690)
                     /locus_tag="VFD06_08670"
     CDS             complement(1653..2690)
                     /locus_tag="VFD06_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN03650.1"
                     /translation="MRDLSPAPFEDAVQLAAAVCAAPLAVAAFPGSDRLSIRASTAPL
                     PAEIPFQFGFSALTVAAREPIVLPDTHLDSRAADNAVLGPPWFARFYAGVPFLTSDGR
                     VLGALEIYDHVPRQLEPWQMDGLLRLARIVTGTIESWSAQRERETRRARTLAALAGGL
                     AAELKRSEAGIGATSRAADLARQLLAVAGHRSLRPQPIDLNALVTGLSRSRPAEEAAG
                     AWSLALDPAAGEVYGDEERIGRAIRGLAARAAGLRREPVPIATRDVVVPGPPADASLL
                     PPGRYVMVTIGAPPPGAEAEISAWFEEPRAGDIAEVSGLMVESGGRLGFRRDPAGGPA
                     YCLALPRILPT"
     gene            complement(2726..2929)
                     /locus_tag="VFD06_08675"
     CDS             complement(2726..2929)
                     /locus_tag="VFD06_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03651.1"
                     /translation="MSRVPAGRARRGLRPRTLLGAFFVLTAASAPRAEDLYIPNQGSN
                     TVTIVDARNLADRRSAPIDAAAD"
     gene            complement(2926..>3711)
                     /locus_tag="VFD06_08680"
     CDS             complement(2926..>3711)
                     /locus_tag="VFD06_08680"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HZN03652.1"
                     /translation="REGAGRALEDRRRAEALLAGLHAARVQGDAVVVPDPESLTGATM
                     TVPIDPALSLPMNAERLFDRAKKGKRAIAAIDTRLAAVERHHAAWADVEARSAAAMTA
                     ADLEPVREAMAALGLVHRAPRPGRPAGAPSQAPTRVRRHTTKDGFVILVGKSGPENDT
                     LTFKVASPWDFWLHAAGHAGAHVVVRNPRRLGSLPEPALRVAAEIAAYYSGAREAGKA
                     EVHVTQRKHVRKRKGMAPGQVLLRRFQSIQVMPRLPGSSLEEV"
BASE COUNT          500 a         1395 c         1376 g          440 t
ORIGIN      
        1 gagcaggaga taggcatccg ccatctgggt gaagtgctcg gggatggtcc ggcacgaacc
       61 gccggaaagc ggcaccgtct ccgccagggc cgcggggggg gtcctgagca cgccgctgta
      121 gaccagctcc cccgaggaca agcgttcccg atcggtacgc ccctctggtt tcggccggcc
      181 gccggagaac ggaacgacgt cggtcgtcgt gctgccgaca tccacgacga gtccttccgg
      241 caggcgccgc gccgcgacgg tcgcgggggc gatccagttg gccgacgcga ccgcgagcgg
      301 cgtcttgcgg gcgtcggcgc tggagacgag gcgtccttcg gcgtggagca ccgagaaggt
      361 gtcatcgccg cgcgcggcgg ccacggcgtc gaggatcgcc cgcacccccg cgctccggtc
      421 ggtgaaggcg tcggacagct cggcggtgag cgtcaccgcg agcgggcccg caggctccat
      481 ccgggccagc tcccccgcga gccgtgacgg ctccctccag atctcgaacg gcctgagccg
      541 ccagtcgagt cgtgtccccg cctccgtgtc gaggagccgg acctgcttga ggtgcacgcc
      601 gccgatgtcc cacccggacg cgagcccctt catgcggccc tcatcgcgtc atgccaggcg
      661 ccggaccggc ccgctcgcgt ggaagcgcca gcgtccgctg gagcggagcc gcgccggcag
      721 ccgctcgccg cgcgccgcgc cgatcatgaa gcgcgcgatc ctcccgcccg ccatccgcga
      781 gagcccgaga tagctcgagg tcagacgcgg gttgatttca atgaccaccg gcccggcctc
      841 ccccaggacg agatcgacgc cgacgaaccc gcggaggtcg ccggcggcgc gggcgagggc
      901 cgccaccgcg gcgcgtgccg cctcctccac catcgcggcc gcctgcggcg gaagccttcc
      961 cgggatctct cccccggtgt acgccagccg gaatcggccg ctcaggtgct ggcatccgag
     1021 accgagacag aggaggtctc cgacccgctc gccgtcgccg ccggcacgca ccaggtatga
     1081 cgcgctgaga gcggttccgg cgacgaaggc ctgcgccacg atcccatcgc cgcgcgccgc
     1141 gcgtgccgcg atccgcgcgc cgcgccggcg gtcctcagga cgccgcacga cgacgactcc
     1201 ttccgccccg cagccgcgcc tcggcttgaa gacgatcgcg ccggcgcgct tccggccggc
     1261 agccgggacc ggcaccccgg cggcgcgcag caggcgcccg gtcgcgatct tgtccgcgcc
     1321 cagccggacg gcccgcggac cggggccgag cgacaggacc ccggcgcgcg cgggggctcc
     1381 ggcgagccgc tcgaggaggc cgccgtcctc gggagcgatg acgagcgcgg catcggcccg
     1441 cgcaagggcg cgatcgagga cgcgccgcgg cgggccgggc cgaagcggca cgagcctcgt
     1501 gccgggaacg gaggcgaggt cgcggctcac ggcgtcgcgc atcgcgcggc cggcacgcag
     1561 gatcgtcctc gccgcgccac gcgacgcgcg ggcgccggga cggctgaagt gctcgtagac
     1621 gacgatgcgc acggccggat ggaacccggg cttcaggtgg gaaggatccg cggaagcgcc
     1681 aggcagtagg ccggtccgcc ggccgggtcc cgccggaatc ccagtcggcc accgctctcg
     1741 accatgagac cgctcacctc cgcgatgtcg cccgcgcgcg gctcttcgaa ccaggcggag
     1801 atctcggcct ccgcgccggg cggcggggcg ccgatggtga ccatgacgta gcggccgggg
     1861 gggagaagcg atgcgtccgc gggagggccc ggcaccacga cgtcccgcgt cgcaatcggc
     1921 accggctccc ttcgcagccc ggcggcgcgc gccgcgagcc cgcggatcgc gcggccgatc
     1981 cgctcttcgt ccccgtacac ctcgccagcg gccgggtcga gcgccagcga ccacgccccc
     2041 gccgcctcct ccgccggccg cgagcgcgac agcccggtca cgagcgcgtt gaggtcgatc
     2101 ggctgcggac ggaggctccg gtgtccggca accgccagga gctggcgcgc cagatcggcc
     2161 gcgcgggagg tggcgccgat cccggcctcg ctccgcttca gctctgccgc cagtcctccc
     2221 gcgagcgccg ccagggtccg ggcgcgccgg gtctcccgct cgcgctgcgc cgaccacgat
     2281 tcgatcgtcc cggtgacgat ccgcgccagt cggagcaacc cgtccatctg ccagggctcg
     2341 agctgccgcg ggacgtgatc gtagatctcg agcgcgccga ggacacggcc gtcggacgtc
     2401 aggaacggaa cgccggcgta gaagcgcgca aaccacggcg gcccgaggac ggcgttgtcc
     2461 gcggcgcgcg aatcgagatg ggtgtcgggg aggacgatcg gctcgcgcgc cgcgacggtc
     2521 agggcgctga acccgaactg gaacgggatc tccgccggaa gcggagcggt ggaggcgcga
     2581 atgctcagcc gatcggaacc ggggaacgcg gcgacggcca gaggggcggc gcacaccgcc
     2641 gcggcgagct gcaccgcgtc ctcgaatggc gccggcgaca gatccctcat acgcgggcgt
     2701 aggttagcgc cgcggtctgc ggccgtcaat cggcggcggc gtcaatcggc gcagaccgcc
     2761 ggtcggcgag atttcgcgca tcgacgatcg tgaccgtgtt ggatccctgg ttcggaatgt
     2821 agagatcctc ggcccgcgga gcggaggcgg cggtgaggac gaagaacgcc ccgaggaggg
     2881 ttcggggacg gagccctctt cgtgcgcgac cggccggcac gcggctcaca cttcctccag
     2941 ggacgatccc ggcaggcgtg gcatcacctg gatcgactgg aagcggcgga gcaggacctg
     3001 ccccggcgcc atccccttgc gcttccggac gtgcttgcgc tgcgtcacgt ggacttccgc
     3061 tttccccgcc tcccgggcgc cgctgtagta cgcggcgatc tccgccgcaa cgcggagcgc
     3121 cggctccggc aggctcccga ggcgacgcgg gttgcgcacc acgacgtggg cgccggcatg
     3181 gccggccgcg tgcagccaga agtcccatgg cgacgcgacc ttgaacgtga gcgtgtcgtt
     3241 ctccggaccg ctctttccga ccaggatgac gaagccgtcc ttggtcgtgt ggcggcgtac
     3301 ccgcgtcgga gcctgcgacg gcgcgccggc cgggcgtccg ggccgcggcg cccggtgcac
     3361 gaggccgagc gccgccatcg cctcccgcac cggctcgagg tcggcggccg tcatggcggc
     3421 ggcacttcgt gcctcgacgt cggcccacgc ggcgtggtgg cgctcgacgg ccgcgagccg
     3481 cgtgtcgatg gcggcgatgg cgcgcttccc cttcttggcg cgatcgaaga gccgctcggc
     3541 gttcatcggc agcgacagcg cgggatcgat cggcacggtc atcgtcgcgc cggtcagcga
     3601 ctccggatca gggacgacga cggcatcccc ctggactcgg gccgcgtgca ggccggcgag
     3661 gagcgcctcg gcgcgccgcc ggtcctcgag ggcccgcccc gccccctccc g
//
LOCUS       DASRIQ010000364         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_927135, whole genome shotgun sequence.
ACCESSION   DASRIQ010000364 DASRIQ010000000
VERSION     DASRIQ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927135"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFD06_08685"
     CDS             <1..360
                     /locus_tag="VFD06_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03653.1"
                     /translation="LAFLAIPMFDMVLFTMFVAAALALRRDLETHKRLMLMAYISIMV
                     AAVARLPGVLAMGPPGFFGLTFIFVLIAGIYDLLTRRRVHKAYLWGGGIFLVSQPLRM
                     MISGTDAWHALAEMLTR"
     gene            395..667
                     /locus_tag="VFD06_08690"
     CDS             395..667
                     /locus_tag="VFD06_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HZN03654.1"
                     /translation="MLFMVIERFRKGQAPAVYRRFRDRGRMAPESVRYVVSWVDLEFQ
                     RCFQVMEAPSEADLKAWTDNWDDLVEFEIVPVRTSAEAAQAIAPQL"
     gene            675..1610
                     /locus_tag="VFD06_08695"
     CDS             675..1610
                     /locus_tag="VFD06_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HZN03655.1"
                     /translation="MSPEARPSGRVVIAGGTGFLGRNLARHLAALGCEVVVLSRHVPR
                     DAPGWRHVPWDARTTGEWVRPLDGAAALVNLVGRTVDCRKTPDHCDEILRSRVEATEA
                     LGRALRQTGTLPRVWVQMSTAHRYGDPPEVLIDEGSAFGYGLAPLVGQAWEEAFARAL
                     PAGVRPVVLRTSFVLGNGGALTRLARLARLGLGGRVGHGRQGISWIHEEDMNRLFARA
                     IADEGMTGAYIATAPNPVSNAEFMRALRGALGVPIGLPAMEWMVRIAAPLFLRTDPEL
                     ALYGRYCVSRRLREEGFEFRFPDLPSALRDLYASP"
     gene            complement(1639..2958)
                     /locus_tag="VFD06_08700"
     CDS             complement(1639..2958)
                     /locus_tag="VFD06_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN03656.1"
                     /translation="MRHGRMFAATVLLIAASIAPAPAAPASEPAPVLTVIRAGTLIDG
                     VVGSARRDQLVFVVGNRIEKVAPGATPIPAGARVIDLSSATVLPGLIDSHTHIFLWGE
                     KPENGGYDANILKPALSLRAARATFACRRALEQGFTTIRDLETEGAGYGDVGIKEAIN
                     EGTIPGPRIYTSTKAISTTGGYNLEGYAPELTMPKGAQIVDGPVEARKAAREQLEHGA
                     DWIKVYMTHRSWVDRQGRLVSQPTLTLEELRAIVDEAHGWQKKVACHAYNGLGMQRAL
                     DGGCDSIEHGLEMTSAQIAQMVKQGTWYCPTIAPYYLSWAPEDTPVGKRDRMRAEVHG
                     PTVRAAVKAGAKIVFGTDMGGIDWTEPMWKEFSYMIEFGMTPMAAIQSATSRAAEMLD
                     AKGDLGVVAAGAYADIIAVNGDPLAGIEALGKVRFVMKDGKVFRDDR"
BASE COUNT          413 a         1026 c         1134 g          501 t
ORIGIN      
        1 ctcgcgttcc tggcgattcc gatgttcgac atggtgctgt tcacgatgtt cgtggccgcc
       61 gccctggcgc tgcgacgcga tctcgagacg cacaagcggc tgatgctcat ggcctacatc
      121 agcatcatgg tggccgcggt cgcgcgtctg cccggggtgc tcgcgatggg gccgccgggc
      181 ttcttcgggc tgacgttcat cttcgttctg atcgcaggga tctacgatct cctgacgcgg
      241 cggcgggtgc acaaggccta cctgtggggc ggcgggatct tcctcgtctc ccagccgcta
      301 cgcatgatga tctccggcac cgacgcatgg catgcgctgg cagaaatgct gacgcgatga
      361 agatgccggc ggcgccggcg ggagggcgca agtgatgctg ttcatggtga tcgagcgctt
      421 ccggaaaggc caggcgccgg cggtgtatcg ccgcttccgg gatcgggggc gcatggcgcc
      481 ggagagcgtg cgctacgtgg tgagctgggt cgacctggag ttccagcggt gcttccaggt
      541 catggaggcg ccgagcgagg cggatctgaa ggcatggacg gacaactggg acgacctggt
      601 ggagttcgag atcgtcccgg tccgcacgtc cgccgaggcg gcgcaggcga tcgcgccgca
      661 gctctgacgc gcccttgagc cccgaggcgc ggccgtccgg acgggtcgtc atcgccggcg
      721 ggaccggctt cctcggccgg aacctggcgc gtcatctcgc cgccctgggc tgcgaggtgg
      781 tcgtgctgtc gcgtcacgtg ccgcgcgacg cgccggggtg gcgccacgtc ccgtgggatg
      841 cccgcaccac cggtgagtgg gtgcggccgc tcgacggcgc cgcggcgctc gtgaacctgg
      901 tcgggcggac ggtggactgt cgcaagacgc cggatcactg cgacgagatc ctgcgctcgc
      961 gcgtcgaggc gaccgaggcg ctcggccgcg cgctcaggca gacggggacg ctgccgcgcg
     1021 tgtgggtgca gatgtccacg gcgcaccgct acggcgatcc gcccgaggtg ctcatcgacg
     1081 aggggagcgc cttcgggtac gggctggcgc ccctcgtcgg gcaggcgtgg gaagaggcgt
     1141 tcgcgcgggc gctcccggcg ggggtccggc cggtcgtgct gcgcaccagt ttcgtgctgg
     1201 gaaacggcgg ggctctgacg cgcctcgccc ggctggcgcg cctggggctc gggggccggg
     1261 tcggccacgg ccggcagggg atcagctgga tccacgaaga ggacatgaac cggctcttcg
     1321 cccgcgccat cgccgacgag ggaatgaccg gcgcgtacat cgcgaccgcg ccgaacccgg
     1381 tgtccaacgc cgagttcatg cgcgcgctgc gtggcgcgct cggcgtcccg atcggcctgc
     1441 cggcgatgga atggatggtg cggatcgccg cgccgctctt cctgcgcacc gatccggagc
     1501 tggcgctcta cggccggtat tgcgtctcgc gccggctgag ggaagaagga ttcgagttcc
     1561 ggttcccgga cctcccgtcg gccctgcgcg acctgtacgc gtcgccctga ggtgccgaag
     1621 aagaaatgac gtcgcgcgtc agcggtcgtc ccggaagacc ttcccgtcct tcatgacgaa
     1681 gcgcaccttg ccgagcgcct cgatgccggc gagcgggtcg ccgttcaccg cgatgatgtc
     1741 ggcataggcg cccgccgcga cgacgccgag gtcccccttc gcgtcgagca tctcggccgc
     1801 gcgcgaggtg gcggactgga tggccgccat cggcgtcatc ccgaactcga tcatgtagct
     1861 gaactccttc cacatcggct cggtccagtc gatgccgccc atgtcggtgc cgaagacgat
     1921 cttcgcgccg gccttgacgg cggcgcgcac cgtcgggccg tgcacctcgg cacgcatccg
     1981 gtcgcgcttg ccgacgggtg tgtcctcggg cgcccaggag aggtagtacg gcgcgatggt
     2041 cgggcagtac caggtcccct gcttcaccat ctgggcgatc tgggcggagg tcatctcgag
     2101 cccgtgctcg atcgagtcgc agccgccgtc gagggcgcgc tgcatcccga ggccgttgta
     2161 ggcgtggcag gcgaccttct tctgccagcc gtgcgcctcg tccacgatgg cccgcagctc
     2221 ctcgagcgtc agggtcggct gcgagacgag acgcccctgc cggtccaccc aggagcggtg
     2281 cgtcatgtac accttgatcc agtcggcgcc gtgctcgagc tgctcgcgcg ccgccttgcg
     2341 cgcctcgacc gggccgtcca cgatctgcgc ccccttcggc atcgtcagct cgggcgcgta
     2401 tccctcgagg ttgtaacccc ctgtggtcga gatggccttg gtggaggtgt agatgcgcgg
     2461 gccggggatg gtcccctcgt tgatcgcctc cttgatgccg acgtcgccgt agccggctcc
     2521 ctcggtctcg agatcgcgga tggtcgtgaa cccctgctcg agcgcccgcc ggcaggcgaa
     2581 ggtggcgcgc gccgcgcgca gcgacagcgc cggcttcagg atattggcgt cgtagccgcc
     2641 gttctccggc ttctcccccc acaggaagat gtgggtgtgg gaatcgatga gaccggggag
     2701 cacggtggcg ctggagagat cgatgacgcg cgcgcccgcg gggatgggcg tcgcgccggg
     2761 cgcgaccttc tcgatgcggt tccccacaac gaagacgagc tgatcgcgcc gggcgctgcc
     2821 gacgacgccg tcgatgagcg tcccggcccg gatcaccgtc aggaccggag caggctcgga
     2881 tgcgggcgcg gcgggcgccg gcgcgatcga tgcggcgatg agcaggactg tcgcggcgaa
     2941 catccgaccg tggcgcatgg ggcctcctcg tggtgcgatg ggctctcggg ggcgaaacgg
     3001 tatcaccaac aggttcatcc cggatggcac ggcgaaggcc atctgtcgta aggttcaccg
     3061 agaaaagggg gtgc
//
LOCUS       DASRIQ010000365         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1711201, whole genome shotgun sequence.
ACCESSION   DASRIQ010000365 DASRIQ010000000
VERSION     DASRIQ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711201"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..176
                     /locus_tag="VFD06_08705"
     tRNA            105..176
                     /locus_tag="VFD06_08705"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Ile,seq:gat)
     gene            390..462
                     /locus_tag="VFD06_08710"
     tRNA            390..462
                     /locus_tag="VFD06_08710"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:423..425,aa:Lys,seq:ctt)
     gene            493..564
                     /locus_tag="VFD06_08715"
     tRNA            493..564
                     /locus_tag="VFD06_08715"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:526..528,aa:Met,seq:cat)
     gene            586..658
                     /locus_tag="VFD06_08720"
     tRNA            586..658
                     /locus_tag="VFD06_08720"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Trp,seq:cca)
     gene            708..781
                     /locus_tag="VFD06_08725"
     tRNA            708..781
                     /locus_tag="VFD06_08725"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:742..744,aa:Pro,seq:cgg)
     gene            829..902
                     /locus_tag="VFD06_08730"
     tRNA            829..902
                     /locus_tag="VFD06_08730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:863..865,aa:Pro,seq:cgg)
     gene            912..984
                     /locus_tag="VFD06_08735"
     tRNA            912..984
                     /locus_tag="VFD06_08735"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:945..947,aa:His,seq:gtg)
     gene            complement(1068..>2754)
                     /locus_tag="VFD06_08740"
     CDS             complement(1068..>2754)
                     /locus_tag="VFD06_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN03657.1"
                     /translation="VLGDERHAPALDDLIRVLEDESPRVRFFAAMSLGKLGRREATPA
                     ILRMVEANNDKDPYLRHAGVMAMLGIKDPDPLRATLSQSKPASRLAVLLTLRRLRSPH
                     VKEFLNDPDPRIVLEAARAINDEPIEEAMPALAALVHKPKGLSEHVLVRAANANYRLG
                     TREAAAALVQLAQNDDVAAPWRVDALKLLGEWEQVPGRNHVTGVWNPLPPRDGAVARA
                     VAAPALAGLLRSAPDDVRVAAAGLAQKLGVDQSDLLFELASAKESSPKVRAAALQSLA
                     GKNDPRLPDAVKAALQDADEAVRLQAIALQAKLPTGASSLIPRLNSGNVREQQAVLAA
                     LAAAADPEADRAIGGTLQRLLSGNLPPEIQLDVLEAAEKRNTPEMSEKLAQHHARRRS
                     DDPLAEYREALAGGDPIAGERIFEERADVSCVKCHSVNGFGGNAGPDLAGVGAKQTRE
                     YLLESILAPPAKIAEGWEAVIVRLKNGEVVSGIVKGETEQELVLNVITDPTRGITRPI
                     VLKKKDIDRRRGGQSAMPEGLVKALTKRELRDLIEYLASLKEPAGVKGEADRG"
BASE COUNT          383 a          948 c          964 g          459 t
ORIGIN      
        1 tcgtgagttc gaccctcacc cgccgcactg cgcgcgtagg cgccccgact gtgcgagcac
       61 ggcgggcttt ggcgggagag tccccgcgcc ctggaatgcg gaactggagc gtagctcagc
      121 gggagagcag cccgccgata acgggcaggt cgtcggttcg actccgaccg ctccaaatga
      181 aggggtggag ggtggagtgg ttcggatccc gtcagagacc ggctctcacg tgagccgtca
      241 tcccgagggg agcggtagcg acccgaggga tctcggggag ggcacacgcg cgtcactcct
      301 gcccgagatc cctcgtcgct gacgctcctc gggatgacgg ctcatcgggc cccagagacg
      361 ttcggggagc cgtatgggat cagacaagcg cagcggtagc tcaattggcg gagccgccgg
      421 ctcttaaccg gaagggtgca ggttcgagtc ctgcccgctg cactggaacg acgggacgtg
      481 aggaagtgac agcggtgcgt agctcaagcg gaagagcggc ggtctcataa gccgcaggcg
      541 ccggttcgac cccggccgca ccgacctgac gagagctcga ttgacgcggc gagagctcga
      601 gcggcagagc agcggtctcc aaagccgcct tgtaggggtt cgagccccct tcgccgtgct
      661 ggacgggacg atcgtttaac cgcgggcgcg agagcgaccg cgacgacagg ggtgtagctc
      721 aacctggcag agcgctcggt tcgggaccga gaaggtggaa gttcaaatcc tcttgcccct
      781 atggatctgg tcggcgtttg cgcgggcgtt gcgagtcccg cggtcgttcg gggtgtagct
      841 caacttggcg gagcgctcgg ttcgggtccg agaggctgga ggttcgaatc ctcctacccc
      901 gactggtgac ggtggccgta gctcaatcgg cggagcgccg ggttgtgagc ccggaggttg
      961 cgggttcgaa ccccgccggt caccctttcg gggaaacgcg aggatagagg atggaggata
     1021 gaagatagcg cggggcaccc tgcttgttct tcgctttcct ctatcttcta tcccctatcc
     1081 gcttccccct tcacccccgc cggctccttc aggctggcga ggtattcgat caggtcgcgc
     1141 agctcgcgct tggtcagtgc cttcaccagc ccctcgggca tcgccgactg gccgccgcga
     1201 cggcggtcga tgtccttctt tttcagcacg atcggccggg tgatcccgcg cgtcgggtcg
     1261 gtgatgacgt tgagcaccag ctcctgctcc gtctcgccct tcacgatgcc ggagacgact
     1321 tcgccgttct tcagccgcac gatcaccgcc tcccagccct cggcgatctt ggccgggggg
     1381 gcgaggatgg actcgagcag gtactcgcgc gtctgcttcg ccccgacgcc ggccaggtcc
     1441 ggccccgcgt tgccgccgaa gccgttgacg ctgtggcact tgacgcagga gacgtcggcg
     1501 cgctcctcga aaatgcgctc cccggcgatc gggtcgccgc cggccagggc ctcgcggtac
     1561 tcggcgaggg ggtcgtcgct gcggcggcgg gcgtggtgtt gcgcgagctt ttcactcatt
     1621 tccggagtat tccgcttttc ggccgcctcc aggacgtcca gttggatctc cgggggcagg
     1681 ttccccgaaa ggagccgctg caacgtgccg ccgatcgcgc ggtccgcttc agggtcggcg
     1741 gcggcggcta aagccgccag gaccgcttgt tgctcgcgga cgttgccgct gttgagccgg
     1801 gggatgaggc tcgacgcgcc cgtcggcagt ttcgcctgca gcgcaatggc ctggaggcgc
     1861 acggcctcgt cggcgtcctg cagcgccgcc ttgacggcgt cgggcaggcg ggggtcgttc
     1921 ttccccgcca gcgactgcag cgccgccgcc cgcaccttcg gcgacgactc cttcgccgac
     1981 gccagctcga acagcaggtc cgactggtcc acgcccagct tctgcgccag gcccgcggcg
     2041 gcgacgcgga cgtcgtcggg ggcgctgcgg agcaggccgg ccagcgccgg cgccgcgacc
     2101 gccctggcca ccgcgccgtc ccgcggcggc agcggattcc acacgcccgt cacgtggttg
     2161 cggcccggca cctgctccca ctcgccgagc agcttcagcg cgtccacccg ccacggcgcg
     2221 gccacgtcgt cgttctgcgc gagttgcacc agcgccgccg ccgcctcgcg cgttcccagg
     2281 cggtagttgg cgttcgccgc gcggacgagc acgtgctcgc tgagcccctt tggcttgtgc
     2341 accagcgccg ccagcgccgg catcgcctcc tcgatcggct cgtcgttgat cgcccgcgcg
     2401 gcctccagga cgatgcgcgg gtcggggtcg ttcaggaatt ccttcacgtg cggcgaacgc
     2461 agccgccgga gcgtgagcag gaccgccagc cgcgacgcgg gcttcgactg cgacaaggtc
     2521 gcacggagcg ggtcggggtc cttgatcccc agcatcgcca tcacgcccgc gtggcgcagg
     2581 taggggtcct tgtcgttgtt cgcctcgacc atccgaagga tcgccggcgt cgcctcgcgg
     2641 cggcccaact tgccgaggct catcgccgcg aagaagcgga cgcgcgggct ttcgtcctca
     2701 agcacgcgga tcaggtcatc gagcgccggg gcgtgccgct cgtcacccag gacc
//
LOCUS       DASRIQ010000366         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3266732, whole genome shotgun sequence.
ACCESSION   DASRIQ010000366 DASRIQ010000000
VERSION     DASRIQ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3266732"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VFD06_08745"
     CDS             <1..1476
                     /locus_tag="VFD06_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZN03658.1"
                     /translation="TGALQTVTYPVANYRFEGARFFTNKPPCGPKRGHGTPQPRFGLE
                     VQLDKIAARLGMNPADLRLKHLVPPNSLTANYLRVGSMGLGACIEKVVAGSGFREKHG
                     RLPRGKGIGLACSSYICGAGLPIYWNSMPHTGVQIRLDRGGGVAVFCGEIDIGQGSDT
                     VLAQFVAEVLGIDPLDIRLCVGDTDLTPVDLGSYSSRVTLMMGNAAIQAAERARELLT
                     KAVAAKLEIPGERVGFGGGRVYDVEDPSAKSMTFAEAVVLAESRFGTIGTVGSYVPPP
                     SAGRYRGAGVGPSPAYSYSAAVVEVDVDPDSGWIKVERVSIAHDVGRSINPVLVIGQV
                     EGSVYMGLGEALMEEQIFRANRQGVHKIPSMLEYKSPTTMEMPEVITWLVEDPDPNGP
                     FGAKEVGQGPLLPVPPAVANAVYDAVGVRVDEVPITPEKILKALDSPQKRYGPTRLPI
                     VQWPETTRVATPWEGGTGKALEPEKAGAGTAGPVGPGGVRR"
     gene            1473..>2214
                     /locus_tag="VFD06_08750"
     CDS             1473..>2214
                     /locus_tag="VFD06_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZN03659.1"
                     /translation="MMRLPEFRYLEPRSLKEAATILAGEGPTAMPLAGGTDLFPNMKR
                     RHQMPKTLVALGGLERLRGVRGGPRGLTLGPSTTLTELEEHEGLRRAWPGLHEAVKSI
                     STPILRNMGTLGGNLCLDTRCNYYNQNYEWRRAINFCMKCDGDTCWVAPGSDTCLAIS
                     SSDTAPMLCAIGATLRLVLKTGERAIPARDLFARDGIRYLTKAPDEILTGVDLPPAGG
                     WRTAYRKLRRREAFDFPVLGVAVCLRFEQ"
BASE COUNT          341 a          754 c          794 g          325 t
ORIGIN      
        1 accggcgcgt tgcagacagt cacgtatccc gtcgccaact atcgcttcga gggggcgcgt
       61 ttcttcacca acaagccgcc gtgcggcccg aagcgggggc acggcacgcc gcagccccgc
      121 ttcggtctcg aggtgcagct cgacaagatc gcggcgaggc tgggaatgaa tcccgctgat
      181 ttgaggttga agcacctggt gcctccgaac tcgctcaccg ccaactatct tcgggttggt
      241 tcgatggggc tcggcgcctg catcgagaag gtggtcgcag gttcggggtt ccgcgagaag
      301 cacgggcggc tgccgcgcgg gaaggggatc ggcctcgcct gctcgtcgta catctgcggc
      361 gccggcctgc cgatctactg gaacagtatg ccgcacacgg gggtgcagat ccgcctcgac
      421 cgcggcggcg gggtggcggt cttctgcggc gagatcgaca tcgggcaggg gtcggacacc
      481 gtcctcgcgc agttcgtggc ggaggtgctc gggatcgatc cgctcgacat ccggctctgc
      541 gtcggcgaca ccgacctcac gccggtcgac ctcgggtcct acagcagccg cgtcacgctc
      601 atgatgggca acgccgccat ccaggcggcg gagcgggcgc gcgagctcct gacgaaggcg
      661 gtcgcggcga agctcgagat ccctggcgag cgggtcggct tcggcggcgg gcgggtctac
      721 gacgtggagg atccgtcggc gaagagcatg acgttcgccg aggcggtggt gctcgcggaa
      781 tcccgcttcg ggacgatcgg cacggtcggg tcgtacgtgc cgccgccgtc cgccggccgc
      841 taccgcggtg ccggagtcgg tccttccccc gcgtactcgt acagcgccgc cgtcgtcgag
      901 gtggacgtcg accccgacag cggctggatc aaagtggaac gggtgtccat cgcgcacgac
      961 gtcgggcgct cgatcaatcc ggtgctggtg atcggccagg tcgaggggag cgtctacatg
     1021 gggctcggcg aggcgctgat ggaagagcag atcttccgcg ccaaccgcca gggggtgcac
     1081 aagatcccgt cgatgctcga gtacaagagc ccgacgacga tggagatgcc ggaggtgatc
     1141 acctggctcg tcgaggaccc ggatccgaac ggcccgttcg gcgccaagga ggtcgggcag
     1201 gggccgctcc ttccggttcc cccggcggtg gccaacgccg tgtacgacgc ggtcggcgta
     1261 cgcgtggacg aggtgccgat cacgccggag aaaatcctca aggcgctcga ctccccgcag
     1321 aagaggtatg gcccgacgcg cctgccgatc gtccaatggc ccgagacgac gcgcgtcgcg
     1381 acgccgtggg agggaggaac cggcaaggcg ctcgagccgg agaaggccgg cgcggggacg
     1441 gccgggccgg tcggccccgg cggggtgcgg cgatgatgcg cctgccggag ttccgttacc
     1501 tcgagcccag gtcgctcaaa gaggcggcga cgatcctcgc gggggagggg ccgaccgcga
     1561 tgccgctcgc tggcggcacg gatctcttcc ccaacatgaa gcgccgccac cagatgccga
     1621 agaccctcgt ggcgctcggg ggcctcgagc ggctgcgcgg cgtgcgtggc ggcccgcgcg
     1681 gcctgaccct cggcccctcg acgaccctga cggagctcga agagcacgag ggtctgcgcc
     1741 gcgcctggcc cgggctgcac gaggcggtga aatcgatctc gacgccgatt cttcggaaca
     1801 tgggaacgct cggcggcaac ctctgcctcg acacgcggtg caactactac aaccagaact
     1861 acgagtggcg ccgcgccatc aacttctgca tgaagtgcga cggcgacacc tgctgggtcg
     1921 cccccgggag cgacacctgc ctggcgatct cctccagcga caccgcgccg atgctctgcg
     1981 ccatcggcgc gaccctgcgc ctcgtcttga agacggggga gcgcgccatc ccggcgcggg
     2041 atcttttcgc gcgcgacggc atccgctatc tcaccaaggc gccggacgag atcctcaccg
     2101 gcgtcgatct ccctcccgcc ggcggatggc gcaccgccta ccgcaagctg cgccgccgcg
     2161 aggcattcga cttcccggtg ctcggcgtcg ccgtctgcct gcgcttcgag cagg
//
LOCUS       DASRIQ010000367         5995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1457981, whole genome shotgun sequence.
ACCESSION   DASRIQ010000367 DASRIQ010000000
VERSION     DASRIQ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457981"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFD06_08755"
     CDS             <1..532
                     /locus_tag="VFD06_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZN03660.1"
                     /translation="VEPGVDIASPRVAITTVDAGTVAFHPNRIVVEQNDHVRWTWTGG
                     LHNTTSGSACVGNGLWNAPLNGPNTGLTRLFPEPPSTIPFFCMPHCGFMTGQVVVTSE
                     ISVLAKESGASVQLDWTGGGGIYRIFRSSNPLFPAATTAVLTAANGTNALTFLDQTGG
                     TPPVGGVNFYLVMNHF"
     gene            728..2413
                     /locus_tag="VFD06_08760"
     CDS             728..2413
                     /locus_tag="VFD06_08760"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZN03661.1"
                     /translation="MDFLTIITPDGGTQRFDLTPVPNLKIGRSSTSDLVLQDLNVSRM
                     HAELVRRPEGYFVLDAGGKNGTFVNDKRIDAPTMLQPGDRVRLGTTTLVFNGHPKSNV
                     EFTDQPLPAGPGTIHLSADDLRTPNLGEMSVIQGTPAPLHRPTGPGSSPSGGQALQII
                     FEADKELVFHRPLDELLEKIMDLTRRAVRFERGLLMLLEGGTLRSHVVRVPEAETGKP
                     IAISRTITDRVIKKQESILTSDALFDERFKAGQSVEIQQIRSLMCAPLWDNREVIGLI
                     YLDSRQKAGLFTLDDLKLLTHLANVAAVKIENARLFEQAVAAERMEQEIQKAAEIQNH
                     LLPSEGPPIEGYEIFGTSLMCRAVGGDYYDYVPLENGCYGLGLGDVAGKGLPAALLMA
                     SFQASLRALSEMGLSPDDTIRRLNRLLCKSIPENRFVTFFYAVLDPKTHVLTYVNAGQ
                     NPPYIVRVAGGSDRLEQSGPPLALLEDTTYKAHRTRLEPGDVLVCYSDGVSEACGPSE
                     EEFGEMRLTEVVARDMTLSAAEHVRTLTEALLSHCAGRTYQDDVTLVVLKRHS"
     gene            2445..3617
                     /locus_tag="VFD06_08765"
     CDS             2445..3617
                     /locus_tag="VFD06_08765"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZN03662.1"
                     /translation="MNVLLLGVGAVGSVIARHLIPHRSVSRLTLADRDTTRALRLTSD
                     LEASTPGLKEKMRVEAVDASDAADLRRLCRDHGLVLGAVVPRFNGAVMDAALAAGAHY
                     LDMAGGEEDQLLRHEAWKKAGLTALHGMGEDPGISNVLARVGADRLDEVESIRVRDGE
                     FSEADDLPLACLFSTETFIEEATSNALHFEAGAYREHPPWTGREMYPFPDPVGPQPVY
                     FMSHEEVDTLPRFIGKGIRNVDFKLAVPDEMRDRLDFLRRIGMTRRDAIDVGGTRVRP
                     LQVLSAVLPQPADLGGRIRGAAIVLVEVEGRAAGKRARHVLHVAMTHEEAFHRMHSTA
                     TAFLTGTGAAAGALAIVTRRLVTPGVLAPEQIDPAPILAILAELGLEVQESSRPLS"
     gene            3653..4324
                     /locus_tag="VFD06_08770"
     CDS             3653..4324
                     /locus_tag="VFD06_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZN03663.1"
                     /translation="MSADVIACERCPRLRRYCMAVAREKVRRHRDEVYWGRPVPGFGD
                     PKARLLVVGLAPAAHGGNRTGRVFTGDASGDWLFGALHAFGFASQELSTGRNDGLRLF
                     DCYISASARCAPPDNKPALDELRRCRPYLEREIALLRRVRVVVALGRVAHETWLKAAG
                     HWDRMTPRDRPPFGHGGEATLPDGTILIASYHPSRQNTQTGKLTRHMWRAIFRAARRH
                     LDAKS"
     gene            complement(4583..>5995)
                     /locus_tag="VFD06_08775"
     CDS             complement(4583..>5995)
                     /locus_tag="VFD06_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZN03664.1"
                     /translation="VDESMLTGEPLPAEKDEGDRVSAGTLNQSGSFLARVTGTGEDTL
                     LMRIVARVAEAQRTRPPIQRLADRVSAWFVPSVVAIAAIAAVAWGIFGPEPRLAHAVL
                     TAIAVLIIACPCALGLATPMSILVATGRGAQAGVLVRDATALERLAGVDMLLVDKTGT
                     LTEGGPRLTHLLPAAPGPDADAVLLKRAAALERGSEHPLASAVLAAAHERGLSFGEGH
                     HIKLLPGRGVEGVVDGETTRLGSEAFLREEGVEPGDDLLARAAAVRSEGGTVVFLASA
                     GRTAGALGFSDPIRRGTEDVVRLLEREGIRIVMVTGDARATAEAVGRRLGVAAIEASL
                     LPEDKLAMVRRHQQDGRIVAMCGDGINDAPALAQADVGIAMGTGADIAMESAGITLVA
                     GDLHAVLRARRLSRGTLRNIRQNLLFAFAYNLLGVPVAAGALYPFFGLLLSPMIAGAA
                     MSLSSVSVIANALRLRRIPL"
BASE COUNT          940 a         2193 c         2017 g          845 t
ORIGIN      
        1 cgtcgagcca ggggtcgaca tcgcctctcc gcgcgtcgcc atcacgaccg tggatgcggg
       61 aacagtcgcg ttccatccca accgcatcgt cgtcgagcag aacgatcacg tccgctggac
      121 gtggacgggt ggcctgcaca acacgacgag cggctccgcc tgcgttggaa acggactctg
      181 gaacgcgcct ctgaacggcc ccaatacggg cctcacgcgt ctcttcccag agcctccgtc
      241 gacgatcccc ttcttctgca tgccccattg cggcttcatg accggccagg tcgtcgtgac
      301 gagcgagatc agcgtgctcg cgaaggaaag cggcgcctcc gtccagctcg actggacggg
      361 cggaggcggg atctaccgca tcttccgcag ctccaacccg ctcttccccg ccgcgaccac
      421 cgcggtcctc accgccgcga acgggacgaa cgcgttgacg ttcctcgatc agaccggagg
      481 cacccccccg gtcggcggcg tcaacttcta cctcgtgatg aaccacttct aaccgtccca
      541 cctgctccac agcccaggtc ctcggccgtc aggtgaggac gcccagcaag gccatcggaa
      601 cgggttgcgg tgggcgggct agagggaacg ggaacgcctc cgccggggcg tcgagactcg
      661 accccgtacc cggcggaggc gctcccgttc cctctgaggc ggcgactacg gtagcattcg
      721 cctccagatg gacttcctga ccatcatcac ccccgacggc gggacgcagc gatttgacct
      781 gacgccggtc ccgaacctca agatcggtcg ctcctcgacg agcgacctgg tcctgcagga
      841 cctgaacgtc tcgcgcatgc acgccgaact cgtgcgccgc ccggaggggt acttcgtcct
      901 cgacgccggg gggaagaacg gcaccttcgt caatgacaag cggatcgacg ccccgacgat
      961 gctgcagccg ggcgatcggg tccgcctcgg cacgacgacg ctggtcttca acggccaccc
     1021 gaagagcaac gtcgagttca ccgaccagcc gctgccggcg gggcccggga cgatccatct
     1081 ctcggccgac gatctgcgca ccccgaacct cggggagatg tcggtcatcc aggggacgcc
     1141 tgcgccgctc caccggccga ccgggcccgg atcgtcgccg tcgggcggac aggcgctgca
     1201 gatcatcttc gaggcggaca aggagcttgt gttccaccgc ccgctcgacg agctgctcga
     1261 gaagatcatg gacctgacgc gccgcgcggt ccgcttcgag cggggcctgc tgatgctcct
     1321 cgaagggggg acgttgcgct cgcacgtggt gcgggtgccg gaggcggaga ccggcaagcc
     1381 gatcgccatc agccgcacca tcaccgaccg ggtgatcaag aagcaggagt cgatcctcac
     1441 ctccgacgcg ctgttcgacg agcgcttcaa ggcggggcag agcgtcgaga tccagcagat
     1501 ccgctcgctg atgtgcgcgc cgctctggga caaccgcgag gtgatcggcc tcatctacct
     1561 cgacagccgg cagaaggcgg ggctgttcac gctcgacgac ctgaaactcc tgacgcacct
     1621 cgcgaacgtg gcggcggtga agatcgagaa cgcgcggctc ttcgagcagg cggtcgctgc
     1681 cgagcgcatg gagcaggaga tccagaaggc ggccgagatc cagaaccacc tgctgccgtc
     1741 ggagggcccg ccgatcgagg gatacgagat cttcggcacg agcctgatgt gtcgcgccgt
     1801 cggcggcgac tactacgact acgtgccgct cgagaacggg tgctacggcc tcggactcgg
     1861 ggacgtggcc gggaaagggc tcccggcggc gcttctgatg gcctccttcc aggcgagcct
     1921 gcgggcgctg tcggaaatgg gtctgtcccc ggacgacacc atccggcggc tcaaccgcct
     1981 gctgtgcaag agcatcccgg agaaccgctt cgtcaccttc ttctatgccg tcctcgatcc
     2041 gaagacccac gtcctgacct acgtcaacgc cgggcagaac ccgccctaca tcgttcgcgt
     2101 cgcgggggga tcggatcgcc tcgagcagag cggaccgccg ctggcgctgc tcgaggacac
     2161 gacctacaag gcgcaccgga cccgtctcga gccgggggac gtcctcgtct gctacagcga
     2221 cggggtgagc gaggcgtgcg gcccgtccga ggaggagttc ggggagatgc gcctcaccga
     2281 ggtcgtggcg agggacatga ccctttcggc ggcggagcac gtccgcaccc tgaccgaggc
     2341 gctcctgtcc cactgcgcgg gacggaccta ccaggacgac gtgacgctcg tcgtcctcaa
     2401 gcgccattcg taatgcgcgg aacgcgaatg gtcggggagg accggtgaac gtcctcctgc
     2461 tcggcgtcgg cgcggtcggc agcgtcatcg cgcgccacct catcccgcac cgatcggtct
     2521 cccgcctgac gctcgccgac cgggacacga cccgcgccct gcggctgacg agcgatctcg
     2581 aggcctccac ccccgggctg aaagagaaga tgcgggtcga ggcggtggac gcctccgacg
     2641 ccgccgacct gcgccgcctc tgccgtgatc acgggctggt cctcggcgcc gtcgtgccgc
     2701 gcttcaacgg cgccgtcatg gacgccgcgc tggccgccgg cgcgcactac ctcgacatgg
     2761 ccggcggcga agaggatcaa ctcctgcgtc acgaggcctg gaagaaggcg gggctcacgg
     2821 cgctccacgg catgggggaa gaccccggca tcagcaacgt gctggcgcgc gtcggggccg
     2881 accggctcga cgaggtcgag tcgatccggg tgcgcgacgg cgagttctcg gaggcggacg
     2941 atctgccgct cgcgtgcctc ttctcgaccg agaccttcat cgaggaggcg acctcgaacg
     3001 ccctgcattt cgaggcgggg gcgtaccgcg agcacccccc ctggacggga cgcgagatgt
     3061 acccgtttcc cgatccggtc ggaccgcagc cggtctactt catgagccac gaggaggtgg
     3121 acaccctccc ccgcttcatc ggcaagggga tccggaacgt cgacttcaag ctggcggtgc
     3181 cggacgagat gcgcgaccga ctcgatttcc tgcgccgcat cggcatgacg cgacgcgacg
     3241 cgatcgacgt cggcgggacg cgcgtccggc cgctccaggt gctgtcggcg gtgctcccgc
     3301 agccggccga tctcggaggg cgcatccgcg gggccgccat cgtcctcgtc gaggtcgaag
     3361 gacgcgccgc ggggaagcgc gcgcgccacg tgctccacgt ggcgatgacc cacgaggagg
     3421 cgttccaccg catgcactcg accgccaccg ccttcctcac cggcaccggg gccgccgccg
     3481 gggcgctggc catcgtcacg cgccggctcg tcacaccggg cgtgctcgcg ccggagcaga
     3541 tcgacccggc gccgattctg gcgatcctgg ccgagctggg gctcgaggtg caggagtcgt
     3601 cccgcccgct ctcctgacgc acctcacgcg ttccgccggt tcgctcgagg ccgtgagcgc
     3661 cgacgtgatc gcgtgcgagc gctgtccgcg gctccgccgc tattgcatgg cggtcgcccg
     3721 cgagaaggtt cggcgacatc gcgatgaggt gtactgggga cgcccggtcc cggggttcgg
     3781 cgatccgaag gcgcggctcc tcgtcgtcgg gctcgccccg gcggcgcacg gaggcaatcg
     3841 caccggccgc gtcttcaccg gagacgccag cggcgactgg ctcttcggcg cgctccacgc
     3901 attcggcttc gcgagccagg agctctcgac cggtcggaac gacggtctgc gcctcttcga
     3961 ctgctacatc agcgcctcgg cgcgctgcgc tccgcccgac aacaaacccg ccctcgacga
     4021 gctgcgtcgc tgtcgtccct acctcgagcg ggagatcgcc cttctgcgcc gcgtgcgtgt
     4081 ggtggtggcg ctcgggaggg tggcgcacga gacatggctc aaggcggccg gccactggga
     4141 ccggatgacg ccgcgcgacc gcccgccgtt cggccacggt ggcgaggcga cgctgcccga
     4201 cggcaccatc ctgatcgctt cgtaccatcc gagtcgacag aacacccaga cgggcaagct
     4261 cacccgccac atgtggcggg cgatcttccg ggcagctcgc cgtcatctgg acgcgaagag
     4321 ctaaaagcca ctccagattc accgcctctg ggaacccgtg aaaccgccac gactctcatc
     4381 tccgcgatcc agccggctgc ccggaagatc gtcgggcacg acaggtcacg cttcgctctc
     4441 ggttgcttgt tagagtggcg atacggcgcg tcggggtccg tctcgggttt gaagaatccc
     4501 cgaagcgccg ggtcggcggg gcaacgggga cgtccccgtt gccccgccca ctcgagagat
     4561 cgggaaggtc tcgaccccgc gactagagcg ggatgcgccg cagccgcagc gcgttggcaa
     4621 tcaccgagac cgagctgaga gacatcgcag caccggcgat catcggactg agcagcagac
     4681 cgaagaaagg atagagagcc ccggcggcca ccggcacgcc gagcaggttg taggcgaacg
     4741 cgaagagcag gttctggcgg atgttgcgga gcgtgccgcg gctcaggcgg cgggcccgca
     4801 ggaccgcgtg cagatccccc gcgacgaggg tgatgccggc gctctccatc gcgatgtccg
     4861 cgccggtccc catcgcgatg ccgacgtcgg cctgcgccag agcgggcgcg tcgttgatcc
     4921 cgtccccgca catggcgacg atgcgcccgt cctgctgatg acgccgcacc atcgcgagct
     4981 tgtcctccgg caggagcgac gcctcgatcg ccgcgacccc caggcgccgc cccaccgcct
     5041 cggccgtcgc gcgcgcgtcc ccggtcacca tcacgatgcg gatcccctcc cgctcgagga
     5101 ggcgcacgac atcttccgtt ccgcgacgga taggatcgct gaaccccagc gccccggcgg
     5161 tccggcccgc cgacgccagg aacaccacgg tccccccttc ggatcgcacc gccgcggccc
     5221 gcgccaggag atcgtccccc ggctcgacgc cctcctcgcg caggaacgcc tcgctcccga
     5281 gccgcgtcgt ctcgccgtcc acgacccctt cgacgccgcg ccccggaagg agcttgatgt
     5341 gatgaccctc gccgaaagag aggccgcgct cgtgcgccgc cgccagcacc gcagaggcca
     5401 atggatgctc gctcccccgc tcgagcgccg cggcccgctt cagcagcacc gcgtccgcgt
     5461 ccggtcccgg cgccgcgggg aggaggtgcg tcaggcgcgg ccccccttcg gtcagcgtcc
     5521 cggtcttgtc cacgagcagc atgtccacgc cggcgagccg ctcgagcgcc gtggcgtccc
     5581 gcaccagcac gccggcctgc gccccgcggc cggtcgcgac gaggatcgac atcggagtcg
     5641 cgagaccgag agcgcagggg caggcgatga tcagcacggc gatcgcggtg aggacggcgt
     5701 gcgccagccg cggctccggc ccgaagatcc cccaggcgac cgcggcgatg gcggcgatgg
     5761 ccacgaccga cggcacgaac caggcggaga cccggtcggc cagccgctgg atcggcgggc
     5821 gggtgcgctg cgcctccgcc acccgcgcca cgatgcgcat cagcagcgtg tcctcccctg
     5881 tcccggtgac gcgcgcgagg aagctcccgg actggttcag cgtcccggcg ctgacccggt
     5941 cgccctcatc cttctcggca ggcagcggct caccggtgag catcgactca tcgac
//
LOCUS       DASRIQ010000368        12501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1968450, whole genome shotgun sequence.
ACCESSION   DASRIQ010000368 DASRIQ010000000
VERSION     DASRIQ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12501
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968450"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..470)
                     /locus_tag="VFD06_08780"
     CDS             complement(84..470)
                     /locus_tag="VFD06_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding ACT domain-containing
                     protein"
                     /protein_id="HZN03665.1"
                     /translation="MKDLAIVLENRPGALAEMGEALGRAGVSIEGGGAWVVGGSGVAH
                     FLFEDGTAARQALEAAGIRVLAEREVVVVRLKQAVPGQLGLLTRRMAEGGVNIEVLYS
                     DHEHRLILIVDDLARGQEVAAAWSVG"
     gene            complement(526..1134)
                     /locus_tag="VFD06_08785"
     CDS             complement(526..1134)
                     /locus_tag="VFD06_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03666.1"
                     /translation="MPGEFARIVILKPKADQAAAFAAGYERHLAWHRDKDDPWTWRGW
                     TFVLGERVGQFMDGTFGHALANFDQAVDPAADSADNAVNVTPYADFVSHGVYERLPAA
                     SAGAVLPDASPFLLLHTYVVVPGQEAAFETAVARVAKGSGQRMSLFRVRVGGPVSQYL
                     LMRPAQSFSGGAALTPLRLPAGLAQQAQSELLRYQPQLSYVP"
     gene            1314..4055
                     /locus_tag="VFD06_08790"
     CDS             1314..4055
                     /locus_tag="VFD06_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN03667.1"
                     /translation="MPLAPGTRLGPHEIVAPAGSGGMGEIYRARDTRLGREVAIKVLP
                     QHLSADPARRERFEREARAVSSLNHPNICVLYDIGAQDTAQGPIDYLVMELLEGETVA
                     DRLLRGAMPIDQVLRHATEIASALARAHRQGIVHRDLKPGNVMLTKSGAKLLDFGLAK
                     LRDGEPDAPTPGGGSMLPTATRNLTTAGTLLGTFQYMAPEQLEGQEADARSDLFAFGT
                     LVYEMASGRRAFEGKSQASLVAAIMGKEPPPLSQVAPLAPVPLDRIVRRCLAKDPDDR
                     WQSASDLAHELREIAGLPAGPAGASSQSDASQAWPRVASSPTGASATTGRRAVSLPIA
                     AALALAAAAASAAAMYFTRPQPAPPPMVRTHVTLPADLGLAAGDGPIAFSPDGTMVVL
                     APSRDLADRPLHLRRLDSLEMQPLAGTEGATYPFWSPDGRFIGFFAGHKLRKIAPTGG
                     AVFTLCDATDGRGGTWSRDGAIVFSPAPFGPLYRVDQAGGTPTAVTTTAAPAVSHRLP
                     SFLPDGKRLLYFVGTSIKSQDNGIYVLDLESGTSRRLLEGESGGRYAPPGWLVFFRER
                     TLMAQRFDPATIALEGEAAPIAEKVRFSPNRWTGTYTMSDTGLLLYQTGSNAPTGRLT
                     WFDLDGKQGGTLGEQIEGFGPRIAPDGRRAAVTQLGSDGRPEIWVYDLVRGLGSRFTF
                     GPGAAMHSVFSPDSRSIAFAAPTEGKILVASLERSGPPRVVIEQRDVTRMPTSWSPDG
                     RFIVFREQTAGTGWNVQQVDAQGGEPKPLMAGPENEDRGLVSPDGRWLAYVSAESGQR
                     DDLYIVPFADPGGRKYQVTSGGVSGVAQPFAWMSGGRQILFQGGDRRLHVVDVTPEGQ
                     GLVIGTARPAFGDRPAPWQWTVHPDGRRILAIVQNEEDQETPLVLVTDWQAGLRAKE"
     gene            4064..6721
                     /locus_tag="VFD06_08795"
     CDS             4064..6721
                     /locus_tag="VFD06_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN03668.1"
                     /translation="MPLAPGTRFGPYAITAALGAGGMGEVYRARDTRLERDVALKVMH
                     EERAGDAQFRARFEREAKSISALNHPHICTLFDVGHQEGALFLVMELIEGESLADRIA
                     KGPLPPAQMLRLGAQIADALDHAHRQGIVHRDLKPGNVMLTKSGAKLLDFGLARAAAE
                     APAPVSTQAVTSLPTQLKPLTREGTVLGTIEYMAPEQLEGQAADARTDLFALGALLYE
                     MATGRRAFEGKNRTSLIAAIVSTQPAPISTVAPMTPRALDHVVRRCLEKDPEDRWQSA
                     RDVAGMLRGIAESGSQADIPGAATPRRKVRERIAWTLAATMAVIAAISFALRPQQGAA
                     GPAEPDVIRSLLPVASGQLQNDTAHTLAVSPDGTMLAWVDSEGGVWRISVRHFDEDDT
                     HPIPGTENAQQLFFSPDSKWLGFFAGGRLKKVSLGGGAPVVVARAVLPRGASWSADDS
                     ILFTPFFYGGVQRVSASGGNIETLTTPDRAKGEVAHRWPHVLPGGKAFLYTIGVGSSW
                     DEARIAVHTIDSKESRVLIQGGYDGRYLPTGHLVYARGEALYAVRFNPESLQLSGDPV
                     RVLQGVARGDSGGVEYSTSDDGRLVYRPAQSRAGVRGALALVDRAGRPLPFRDPVPTG
                     RSIKFPALSPRGDSLAGEVEYELWNFDLVRGSSTRLTSGTRSTAGVWSPDGALIAFAQ
                     ERNSPWNPYLRRADGSAPEELLVKSDTALQPSAWSPDGHRLLMARTDPGTGLDILSCS
                     LADKTLEPFVATDSNEIAARFSPDGTWVAYTSDESGRDEIYVRPARGATGRWQISTQG
                     AETSYWKKPNEIIFQSGRQVMSVRVRTSPAFSVEPPRVLFEGPDNLLEVMPDGERFLV
                     RQATESGSRDDLLHLVTGWFTEVDRQTRR"
     gene            complement(6718..7167)
                     /locus_tag="VFD06_08800"
     CDS             complement(6718..7167)
                     /locus_tag="VFD06_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN03669.1"
                     /translation="MSVGEPASSQEPILIGQMKITYLIDGSARGGLGVFELCVPPNSN
                     VPPPHSHTANEECVYVLDGVLRYAVNDVTRDLRPGEWMSTPRGSVHQFSNPHDVPARA
                     LIIMTPDIGAQYFRDVGAIVNVPGPPDRARLLEVMSRYGLVPAPPKH"
     gene            complement(7252..8355)
                     /locus_tag="VFD06_08805"
     CDS             complement(7252..8355)
                     /locus_tag="VFD06_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HZN03670.1"
                     /translation="MDSLVRDVCGKTVVMLGEEPHHGGGHTLEIKSELVQRLIARCGF
                     NAIYFESGVYEFVDLDRRLAAGTSAPEQVADAIGGLWSVSRAIDPLVGYLYAQASAGR
                     IRLAGLDPQLVSATSGYEKAALVDDLTQGLNEPRRSTCAEVISRRTNWRYDAEHPDDA
                     AERARLEECFRDAGDAAQPDSVVSVLARTAHAILTNPPGGGPGWDTRERQMAHMFRWQ
                     QRQSPGASRAIVWTANVHASRYLESVSGPVRSFGDELQAEYGDRLASIAFTALGGAYG
                     RRDSTPIAAGPESLEARALAGGAESLRYLPKAALAELGAVDALLLNYDKARRADWSLL
                     FDGAVVLREEKPLSSDRPPRPRFVPVGGERPGR"
     gene            complement(8541..9428)
                     /locus_tag="VFD06_08810"
     CDS             complement(8541..9428)
                     /locus_tag="VFD06_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN03671.1"
                     /translation="MAEPLFRVTLLGTGNPRPSIDRFGPAILVEAGDTRLLFDCGRGV
                     AQRLFQIDGAPLLRSIDAVFLTHLHSDHVVGLPDLWLTGWLFGRHDPLPVFGPKGTAE
                     MATHLSAAFAFDLKVRQQDEGLPPNGSRLKARDGAPGVLFQHAGLSVVAFEVDHGPVR
                     PAFGYRIEFAGRTVVLSGDTRATDVVAARAKGADLLVHEVIAPEAERRLAAVTDPKTI
                     ERILARHATPEECGRLFAKARPRLAVYSHIVPSPATDEDLLPATRKFYDGPLEVGRDL
                     MTITIGAKIEVTTRDGTVR"
     gene            9590..9862
                     /locus_tag="VFD06_08815"
     CDS             9590..9862
                     /locus_tag="VFD06_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HZN03672.1"
                     /translation="MKTYIIERDIPKVGSLDRQQYKEAAAKSNAALRELGFDIQWVES
                     FVAADKTFCVYRASNEDIIRKHAEISGFPATRITAVRRKIDPTTAS"
     gene            9921..10304
                     /locus_tag="VFD06_08820"
     CDS             9921..10304
                     /locus_tag="VFD06_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03673.1"
                     /translation="MNRTGRTSAAAILCAALVLPGVGGVVLGLHHGHSAHHHHEPPLH
                     DAEALDLIVHGHHHEPGAPAHQHRFALGKQAPATTKPSILLDAPALLPCAAVAARLAA
                     GSRTTPGGAAHAPPPGTTVSPVLRI"
     gene            10394..11668
                     /locus_tag="VFD06_08825"
     CDS             10394..11668
                     /locus_tag="VFD06_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN03674.1"
                     /translation="MHRRFVAAVLACSLTPALRAQEVPETGSALPAGIVSEAEFIAPL
                     GEGHVAVRALADDLARAEGASARARTLVNPRLDFWREAPDDHPEVTNWTLAWTPPLDG
                     RHGLGKKATEAGLEAARTRFEIDRAGLRTEFRRVFAEWSLAVERGRALTEQQDLVAGL
                     AERARERARVGEESGLSARRFALARSETATALGEAEAARARAEAEALALRPDLAAGSR
                     PVTPDLPEPPVALDPGQSARLAALEKDKVRTDYEARRVSRFVSFPTLQVGWQTLEDAG
                     VDAGGPILAATWAVPLFDRDQGARVEADRVRDAAEARLTFARDRIAGEIEGRLAAYRT
                     LYAAAGEAARVAGETSQVIEAATAAYQAGESSLTDLLDTLRSAFAARLRAIDARAGAL
                     AAHRDLEEALGRPLAVGGSLTDGGHLTEGGHR"
     gene            11665..>12501
                     /locus_tag="VFD06_08830"
     CDS             11665..>12501
                     /locus_tag="VFD06_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03675.1"
                     /translation="MMRAARAGALLLLLVALGCRREKSAHEAAHAEDEGWAVTAWGER
                     YEVFPECDPLIAGAAATCNAHVTVLEGFKPLVSGAVSVVLRGAGPEQVFRQDHAKRDG
                     IFAVELRPATEGPFDLLFRIEGPAGMEEIPGGRVQVGSAASPGGWAGERAPAPADAVP
                     FLKEQQWKTGFATAPVAEGSLDESVSGPGRVTPAAGGEVALTATFDATVASRPWPYAG
                     LDVKKGAVVFRLQPRVGGRSLPELRAESESLEAETATARRRVERLTELLRSEATSAAE
                     LER"
BASE COUNT         1947 a         4385 c         4384 g         1785 t
ORIGIN      
        1 gcatgtgcac cacgatagct gtttatgaat gcgatgaagt ggcatgggcc atgtccgctc
       61 tgctgctcga agcgcgtcgt ccatcatcca accgaccagg ccgcggcgac ctcctgcccg
      121 cgcgccaggt cgtccacgat caggatcagt cgatgctcgt gatcgctgta gaggacttcg
      181 atgttgacgc cgccttccgc catgcgccgc gtgaggagac cgagctgacc ggggacggcc
      241 tgcttcaatc gcacgacgac cacctcccgc tccgcaagga cgcgaatgcc ggccgcttcc
      301 agcgcctggc gggcggccgt cccgtcctcg aagaggaaat gggccacccc ggagccgccg
      361 acgacccagg ccccgccccc ttcgatgctc accccggcgc gccccagcgc ctcgcccatc
      421 tcggcgagcg cgccggggcg attctcgagg acgatggcga gatccttcat cgcgtgcgcg
      481 aggttagcac tgatcgacgg ggacaaagcg ccgccgcgag gcgtctcagg gcacgtagct
      541 gagctgcggt tgatacctga ggagctcgct ctgggcctgc tgagcgagtc ccgccggcag
      601 tcgaagcgga gtcagcgcgg cgccgcccga aaaggactgg gccggacgca tcagcagata
      661 ttgcgagacc gggccgccga cgcgcaccct gaacaacgac atccgctggc cggacccctt
      721 cgcgacgcgg gcgaccgccg tctcgaaggc ggcttcctgg cccgggacga cgacgtaagt
      781 gtgcaggagg agaaacgggg acgcatccgg gagcacggcc cctgcgctcg ccgccggcag
      841 gcgctcatag acgccatgac tgacgaaatc cgcgtacggg gtgacattca cggcgttatc
      901 cgcactatcc gccgcgggat cgaccgcctg atcgaagttg gcgagcgcat ggccgaacgt
      961 cccgtccatg aactgcccga cacgctcgcc cagcacgaag gtccagcccc gccaggtcca
     1021 gggatcgtcc ttgtccctgt gccacgcgag atgacgctcg tatcccgcgg cgaaggccgc
     1081 agcctggtcc gcctttggct tgaggatgac aatgcgcgcg aactcgccgg gcaactgctc
     1141 agcgtgaggc gaggatgaga tccaggatag gaggaggaga gccgtcaagg caggcatcgc
     1201 acgaaggcgc atccgcgaac ccctatggca atgacttgcg cagcctacag cgcagaccgg
     1261 gcgcgcggcc agagcggcga ggctcggccc ctctggtaga ctccgcgcac aagatgccgc
     1321 tcgcgccggg gacccgcctc ggacctcatg agattgtcgc gcccgccggc agcggcggca
     1381 tgggggagat ctaccgcgcg cgcgacaccc ggctggggcg ggaggtcgcg atcaaggtgc
     1441 tgccgcagca cctctccgcc gacccggccc ggcgcgagcg cttcgagcgc gaggcgcgcg
     1501 ccgtctccag cctgaaccat ccgaacatct gcgtcctcta cgacatcggc gcgcaggaca
     1561 cggcccaggg gccgatcgac tacctggtga tggagctcct cgaaggggag acggtcgccg
     1621 accggctgct gcgaggtgcg atgccgatcg atcaggtcct ccggcacgcg accgagatcg
     1681 cctcggcgct ggcgcgcgcc caccggcagg gaatcgtcca ccgggacctc aagccgggga
     1741 acgtcatgct gacgaaatcg ggcgcgaagc tcctcgactt cggtctggcg aagctgcgcg
     1801 acggcgagcc cgacgcgccg acccccggcg gcggctcgat gctgccgacc gcgacccgca
     1861 atctgacgac cgccgggacg ctcctcggca ccttccagta catggcgccg gagcagctcg
     1921 aggggcagga ggcggacgcg cgcagcgacc tcttcgcctt cggcaccctg gtgtacgaga
     1981 tggcgagcgg ccggcgggcc ttcgaaggga agagccaggc gagcctcgtc gccgccatca
     2041 tggggaagga gccgccgccg ctgtcgcaag tcgcgcccct cgccccggtg cccctcgacc
     2101 ggatcgtccg gcgctgtctc gccaaggatc ccgacgaccg ctggcagagc gcgagcgacc
     2161 tggcgcacga gctgcgggag atcgcgggcc tgccggccgg cccggcggga gcgtcgagcc
     2221 aatcggacgc gagccaggcg tggccccgcg tcgcgtcgtc cccgacaggc gcctcggcaa
     2281 cgaccggccg gcgggcggtg agcctcccga tcgcggccgc cctcgcgctc gctgcggcgg
     2341 ccgcctcggc cgcggcgatg tatttcaccc ggccgcaacc ggccccgccg ccgatggtcc
     2401 ggacgcacgt gacgctgccg gcggacctcg gcctcgcggc cggggacggg ccgatcgctt
     2461 tctcccccga tggaacgatg gtcgttctgg ccccctccag ggacctggcg gatcggccgc
     2521 tgcacctccg gcggctcgac agcctcgaga tgcagccgct cgcggggacg gagggggcga
     2581 cgtatccgtt ctggtccccc gacggcaggt tcatcgggtt cttcgccggc cacaagctcc
     2641 ggaagatcgc cccgaccggc ggcgcggtgt tcacgctgtg cgacgcgacg gacgggcggg
     2701 gcggcacctg gagccgggac ggggccatcg tcttctcgcc ggccccgttc ggtcccctct
     2761 accgcgtgga ccaggccggt ggaaccccca cggccgtgac gacgacggcc gcgcctgccg
     2821 tgtcgcaccg gctcccgtcc ttcctgccgg acggcaagcg cctgctgtac ttcgtgggga
     2881 cgtcgatcaa gtcgcaggac aacgggatct acgtgctcga cctcgagtcc gggacatccc
     2941 ggcgcctact cgaaggcgaa agcggcggtc gttacgcgcc gcccgggtgg ctcgtcttct
     3001 tccgcgagcg gacgctgatg gcgcagcgat tcgaccccgc cacgatcgcg ctcgaagggg
     3061 aagccgcgcc gatcgccgaa aaggtccgtt tctccccgaa ccgatggacc gggacgtaca
     3121 cgatgtccga cacgggcctg ctcctgtacc agacgggatc caacgctccg acgggccggc
     3181 tcacctggtt cgacctcgac gggaagcagg gcggaacgct cggcgaacag atcgaagggt
     3241 tcgggccgcg catcgcgccg gatggacgcc gcgccgccgt cacgcaatta ggctccgacg
     3301 ggcggcccga gatctgggtg tacgacctgg tgcgcggcct cggatcccgg tttacgtttg
     3361 gtcccggagc cgcgatgcac tctgtcttct cccccgactc ccgatccatc gcgttcgcag
     3421 cccccaccga agggaagatc ctcgtggcct ccttggagcg ttccgggccg ccgcgcgtcg
     3481 tcatcgagca gcgggacgtc acccgcatgc cgacctcatg gtcacctgac ggccggttca
     3541 tcgtctttcg ggagcagacg gcgggaaccg gctggaacgt gcagcaggtc gatgcacagg
     3601 ggggggaacc gaaaccgctc atggccggac ccgagaacga ggaccgcggt ctggtctccc
     3661 ccgacggacg gtggctcgcc tatgtctcag ccgaatcggg ccagagggat gatctgtaca
     3721 tcgtcccgtt cgccgatccg ggcggccgca agtatcaggt cacctccgga ggggtgtccg
     3781 gcgtggcgca gccgttcgcc tggatgtccg gcggacgcca gattctcttc caggggggcg
     3841 atcgccgatt gcacgtcgtg gacgtgacac cggaaggaca ggggctcgtc atcggcacgg
     3901 cgcgccccgc cttcggcgac cgcccggcgc cctggcaatg gaccgtccac cccgatggcc
     3961 gccgcatcct cgcgatcgtg cagaacgagg aggaccagga gactccgctc gtcctggtca
     4021 ccgactggca ggcgggattg cgggcgaagg agtagagacc agagtgccgc tcgctccagg
     4081 gacgcgcttc gggccctacg cgatcacggc cgcgctcggc gcgggtggca tgggagaggt
     4141 ctatcgcgcg cgcgacacgc gcctcgagcg cgatgtcgcc ctcaaggtga tgcacgagga
     4201 gcgcgccggc gacgcgcagt tccgcgcccg gttcgagcgc gaggcgaaga gcatctcggc
     4261 actgaaccat ccgcacatct gcaccctctt cgacgtcggt caccaggagg gcgctctctt
     4321 cctggtcatg gaattgatcg aaggggaatc gctcgccgac cgcatcgcga aaggccccct
     4381 gccgcccgcg cagatgctgc gcctcggcgc ccagatcgcg gacgcgctgg atcacgcgca
     4441 ccggcagggg atcgtccacc gcgatctgaa gcccgggaac gtgatgctga cgaaatcggg
     4501 agcgaagctc ctcgatttcg ggctggcgcg cgcggccgcc gaggcgccgg ccccggtgtc
     4561 gacgcaggcg gtcaccagcc tgcccacgca gctgaagccg ctgacccgcg aagggaccgt
     4621 tctcgggacc atcgagtaca tggcgccgga gcagctcgag ggacaggcgg cggacgcgcg
     4681 caccgatctg ttcgccctcg gagcgctgct ctacgagatg gccaccgggc gccgggcgtt
     4741 cgaagggaag aaccgcacga gtctcatcgc cgcgatcgtg tcgacgcagc ctgcgccgat
     4801 ctcgacggtg gcgccgatga ccccgcgggc gctggaccac gtcgtgcggc gctgcctcga
     4861 gaaggatccg gaggaccggt ggcagagcgc acgcgacgtg gccggtatgc ttcgggggat
     4921 cgccgagtcc ggatcgcagg ccgacatccc cggtgcggcg acgccgcgac gaaaggtccg
     4981 tgagcggatc gcctggacgc tcgcggcgac gatggcggtc atcgcggcga tctcgttcgc
     5041 gctgcgcccg cagcagggcg ccgcgggccc tgcggagccg gacgtgatcc gctcgctgct
     5101 tcccgtcgcc tccggacagt tgcagaacga cacggcccac accctggcgg tctcgccgga
     5161 cgggacgatg ctggcctggg tggactccga aggcggagtc tggcgcatct ccgtgcgaca
     5221 tttcgacgag gacgacacac atccgatccc gggaaccgag aatgcccagc agctcttttt
     5281 ttctcctgac tcgaagtggc tcggcttttt cgccgggggc cgactgaaga aagtgtctct
     5341 gggcggcggc gctccggtgg tcgtggcccg cgccgtcctg ccgcgcggcg cctcctggtc
     5401 cgccgatgac agcatcctct tcacaccgtt tttctacggc ggcgtccagc gcgtcagcgc
     5461 atccggtgga aacatcgaaa cgctgaccac tccggatcgc gccaagggtg aagtggcgca
     5521 tcgctggccg cacgttcttc ccggggggaa agcgtttctc tacaccatcg gggtggggag
     5581 cagctgggac gaggcccgga tcgcggtgca caccatcgat tcgaaggaga gccgcgtcct
     5641 gatccagggg ggctatgacg ggcggtatct tccgaccggt cacctcgtct acgcccgcgg
     5701 ggaggcgctg tacgcggtcc gtttcaatcc ggaatcgctg cagctcagcg gcgatccggt
     5761 gcgggtgttg cagggtgtcg ctcgcggcga ttcgggaggg gtcgaatact cgacgtcgga
     5821 cgacggtcgg ctcgtctacc ggccggcgca gagtcgcgcc ggagtccggg gcgctctggc
     5881 gctcgtcgac cgggccgggc ggccgcttcc attccgcgac ccggtgccca cggggcgatc
     5941 catcaaattc cccgcgctgt cgccccgcgg cgacagcctc gcaggggagg tcgaatacga
     6001 gctttggaat ttcgatctcg tccggggatc ctccacgcgc ctgacgtcgg gcacgcgctc
     6061 caccgcgggg gtctggtccc ccgacggagc gctcatcgct ttcgcgcagg agaggaacag
     6121 tccctggaat ccctatctgc gccgggcgga cggcagcgcg cccgaggaac tcctggtgaa
     6181 gagcgacacc gcgctgcagc cgagcgcctg gtcgcccgac ggccaccggc tgctgatggc
     6241 ccggaccgat ccgggcaccg ggctcgacat cctctcctgt tccctggcgg acaagaccct
     6301 cgagccgttc gtcgccaccg acagtaacga gatcgcagcc cgtttctcac cggacggcac
     6361 ctgggtcgcc tacacctccg acgaatcggg tcgcgatgag atctatgtcc ggcccgccag
     6421 gggtgcgaca gggcgctggc agatctccac tcagggggcc gaaacgtcct actggaagaa
     6481 accgaacgag atcatcttcc agagcggccg acaggtcatg agcgtccgcg tccggacctc
     6541 tcccgccttt tccgtggaac cgccgcgcgt gctgttcgag ggtcccgaca acctcctcga
     6601 agtcatgccc gacggcgagc ggttcctggt ccgtcaggcc acggaatcag ggagccgcga
     6661 cgacctgctg catctggtca cagggtggtt caccgaggtc gaccgccaga cccgtcgcta
     6721 gtgcttcggt ggtgccggaa ccagcccgta cctggacatc acctcgagga gtctggcccg
     6781 atcgggggga ccagggacat tcacaatggc acccacatcg cgaaagtact gagcgccgat
     6841 gtccggggtc atgatgatga gtgcgcgggc aggcacatcg tgcggattgc tgaattgatg
     6901 caccgagccg cgcggcgtcg acatccactc cccgggacgc aagtcccggg tgacgtcgtt
     6961 cacggcatac cgcagcacgc cgtccaggac gtagacgcac tcctcgttcg ccgtgtggct
     7021 gtgcggaggc ggaacgttcg agttcggcgg aacgcagagc tcgaagacgc ccagcccgcc
     7081 acgggccgag ccgtcgatga gataggtgat cttcatctgg ccgatcagga tcggttcctg
     7141 cgatgacgcc ggctcaccga cgctcatagc gatctccttg tcacactcgt tccgacatcc
     7201 taccggggca cggagcctgg aagccagagg ggtcggttcc acccggcggc atcatcgtcc
     7261 gggccgctct ccgccgactg gaacgaaacg aggcctgggc gggcggtcgc tggacagggg
     7321 cttctcctcg cgcagcacga cggctccgtc gaacagcaag gaccagtctg cgcgacgggc
     7381 cttgtcgtag ttgagcagca acgcatcgac cgcgcccagc tcggcgagag ccgccttggg
     7441 gaggtaccgc agcgactcgg cgccaccagc cagcgcccgc gcttccaggg attccggtcc
     7501 cgccgcaatc ggggtggagt cgcggcggcc gtaggctccg cccagtgcag tgaacgcgat
     7561 gctcgccagt cggtcgccat actcggcttg cagctcatcg ccaaaggaac gcacagggcc
     7621 gctgacgctc tccaagtatc tggacgcatg gacattcgcg gtccagacga tcgccctgga
     7681 agcgcccggc gactgccgct gttgccatcg gaacatgtgg gccatctgac gttcgcgcgt
     7741 gtcccacccg gggccgccgc caggaggatt cgtcaagatg gcatgggcgg tccgcgcgag
     7801 cacggaaaca acggagtccg gctgggccgc atccccggcg tcgcggaagc actcctccag
     7861 acgggcgcgt tcggccgcat catctggatg ctccgcgtca tagcgccagt tggtacgccg
     7921 cgagatgacc tcggcgcacg tcgaccggcg cggctcattc agcccctggg tcagatcgtc
     7981 caccagcgcc gccttctcgt aacccgaggt cgcgctcacc aactggggat cgaggccggc
     8041 aaggcggatc ctgccggcgg aagcctgggc atacaggtag ccgacgagcg gatcgatggc
     8101 gcggctcaca ctccacagac cgccgatcgc gtcagccacc tgctcggggg ccgacgttcc
     8161 ggccgccagg cggcgatcca gatcgacgaa ctcgtagacg ccgctctcga agtagatcgc
     8221 attgaagccg catcgcgcga tcaaccgttg caccagctcg gacttgatct ccagggtgtg
     8281 gcccccgccg tggtggggct cctcgccgag catcaccacg gtcttgccgc agacatcgcg
     8341 gaccaggctg tccaattgaa cggaggatcc ggtgtccggg ccgcctcggg ggggcccctg
     8401 ggtcgccgga tgttcgcgcg gcaccgcaca tccgcctgtc aagaccgaca ccaacacgag
     8461 gagcagcagg cgcgaactct gcgacgtgag acaggcagct cgtctattca ccgatctccg
     8521 ggcaggggct ccttgccccg tcagcgcacc gtcccgtcgc gcgtggtcac ctcgatcttc
     8581 gcgccgatgg tgatcgtcat caggtcgcgt cccacctcga gcggaccgtc gtagaacttc
     8641 cgtgtggcgg ggaggaggtc ctcatcggtc gccggggacg gcacgatgtg cgagtagacc
     8701 gccaggcgcg gccgggcctt cgcgaacagc cggccgcact cctccggggt cgcgtggcgc
     8761 gccaggatcc gctcgatcgt cttcgggtcg gtcaccgccg cgagccgccg ctcggcctcc
     8821 ggagcgatca cctcgtggac gagcaggtcg gcccccttgg cccgggccgc cacgacgtcg
     8881 gtcgcgcgcg tgtcccccga aaggacgacc gtgcgcccgg cgaactcgat gcgatagccg
     8941 aacgccggcc gcaccggtcc gtgatcgacc tcgaacgcca ccacgctcaa cccggcgtgc
     9001 tggaacagga cgcccggggc gccatcgcgc gctttcagcc ggcttccgtt cggcggaaga
     9061 ccctcgtcct gctgcctgac cttgaggtcg aaggcgaacg ccgcgctcag atgggtggcc
     9121 atctccgccg tccctttcgg cccgaacacc ggcagcgggt cgtggcgccc gaaaagccat
     9181 ccggtcagcc acaggtcggg gagcccgacg acgtggtcgg agtgcaggtg cgtcaggaag
     9241 acggcgtcga tcgagcgcag cagcggcgcg ccgtcgatct ggaacagccg ctgcgccacg
     9301 ccgcgtccgc agtcgaagag aagccgcgtg tcgcccgcct ccacgagaat ggccgggccg
     9361 aagcgatcga tcgacgggcg cgggttgccg gtcccgagga gcgtcacgcg gaagagcggt
     9421 tccgccacct caggcgtcgc cggtgccggc gcaccgggag aagcgaccaa caggacagcc
     9481 agcatggcgg cgcctccgga cacacgcgga tggtagccga agcccgggcg cgttagaatc
     9541 ccggcttcct gtgcacgccg ggcgggcgag tccaagcggg agggacacct tgaagaccta
     9601 catcatcgag cgggacatcc ccaaggtggg gtcactcgat cggcagcagt acaaggaggc
     9661 ggcggcgaaa tcgaacgcgg cgctgcgcga gctgggcttc gacatccagt gggtggagtc
     9721 gttcgtcgcc gccgacaaga cgttctgcgt gtaccgcgcc agtaacgagg acatcatccg
     9781 caagcacgcg gagatcagcg gcttcccggc caccaggatc accgcggtcc gaaggaagat
     9841 cgacccgacg acggcttcct gacgcagggc gccggccggc ccctgtggac ttaccccgga
     9901 ccagtcgtta tattgcacgc atgaaccgga cgggacggac gagtgccgcg gcgatcctct
     9961 gcgccgcgct cgtgcttccc ggggtcggcg gcgtcgtcct cgggctgcac cacggacatt
    10021 cggcgcacca tcaccacgag ccgccccttc acgacgccga agcgctcgac ctgatcgtcc
    10081 acgggcacca tcacgagccg ggcgcgccgg cgcaccagca tcgtttcgcc ctggggaagc
    10141 aggccccggc gacgaccaag ccgtccatcc tcctcgacgc gccggccctt ctgccgtgcg
    10201 cggcggtcgc cgcgcgtctc gcggcgggat cccgcaccac gcctggaggc gccgcacacg
    10261 ccccgcctcc ggggaccacc gtctcacccg tcctccgcat ctgattccgt tcgaagcctg
    10321 aggtctgttt cgtcgcgccg cggcatcgat ccgcgtcgcg gccggtttcg ttctgaatca
    10381 cggaggaact tccatgcatc ggcgattcgt ggcggccgtg ctcgcctgtt cgctcacccc
    10441 cgcgctccgg gcacaggagg tcccggagac cggatcggcc ctgccggccg ggattgtctc
    10501 ggaggccgag ttcatcgccc cgctcgggga aggtcacgtc gccgtccggg cgctggccga
    10561 tgatctcgca cgggccgaag gggcgagcgc gcgggcgcgg acgctcgtca atccgcgcct
    10621 cgacttctgg cgggaagcgc cggacgacca ccccgaggtc accaactgga ccctcgcctg
    10681 gacgccgccg ctcgacggac gccacggcct gggaaagaag gcgaccgagg cgggtctgga
    10741 ggcggcgcgg acgcgcttcg agatcgatcg cgccggcctg cgaaccgagt tccgccgggt
    10801 gttcgccgag tggagcctcg cggtggagcg aggccgcgcg ctgaccgagc aacaggatct
    10861 ggtcgcgggg ctcgccgagc gcgcccggga gcgggcgcgg gtcggggagg aatcgggtct
    10921 ctcggcccgg cgtttcgcgc tggcgcggag cgagaccgcg acggcgctcg gcgaggcgga
    10981 ggcggcgcgg gcgcgcgcag aagccgaggc gctcgcactg cggccggatc tcgccgccgg
    11041 gtcccgcccc gtcactcccg atctccccga gcctccggtg gcgctcgacc cggggcagtc
    11101 ggcgcggctc gcggcgctcg agaaggacaa ggtgcgtacc gactacgaag cgcgccgcgt
    11161 ctcccgcttc gtctcgttcc cgaccctcca ggtcggctgg cagacgctcg aggatgccgg
    11221 tgtcgacgcc ggtggaccga tcctcgcggc gacctgggcg gtgccgctgt tcgatcggga
    11281 ccagggggcc cgggtcgagg cggatcgggt gcgcgacgcg gccgaggccc ggctcacctt
    11341 cgcgcgcgac cggatcgcgg gggagatcga ggggcgcctc gccgcctaca ggacgctgta
    11401 cgccgcggcc ggcgaagcgg cccgcgtcgc cggagagacc agtcaggtga tcgaggcggc
    11461 gaccgctgcc taccaggcgg gtgagtccag cctgaccgat cttctcgaca ctctgcgctc
    11521 ggcgttcgcg gcgcgcctcc gggcgatcga cgcgcgcgcc ggcgccctgg cggcgcatcg
    11581 cgatctggag gaggcgctcg gccggccgct cgcggtcggg ggaagcctca cggatggggg
    11641 acacctcacg gaagggggac accgatgatg cgcgccgcac gggccggagc gctccttctg
    11701 ctgctggtgg cgctcggctg ccggcgtgag aagtccgccc acgaggccgc ccacgccgag
    11761 gacgagggct gggccgtgac cgcatgggga gagcgttacg aggtcttccc cgagtgcgac
    11821 ccgctgatcg cgggagcggc tgcgacgtgc aacgcccacg tcacggtgct cgagggattc
    11881 aagccgctgg tcagcggcgc ggtgagcgtc gtactgcgcg gcgcggggcc cgagcaggtg
    11941 ttccgccagg accacgcgaa gcgggacggg atcttcgccg tggaactgcg tccggcgacg
    12001 gagggtccgt tcgacctgct tttcaggatc gaaggaccgg cggggatgga ggagatcccc
    12061 ggtggccggg tgcaggtcgg cagcgcggct tcaccggggg gatgggccgg cgaacgcgct
    12121 cccgcccccg ccgatgcggt gccgttcctc aaggagcagc agtggaagac cgggttcgcg
    12181 accgcgccgg tcgccgaggg gtcgctcgac gagagcgtct ccggccccgg gcgcgtcacg
    12241 ccggcggcgg gaggggaggt cgcgctcacc gccacgttcg acgccacggt ggcttcgagg
    12301 ccctggccct atgccggtct cgacgtgaaa aagggggctg tggtgttccg gttgcagccg
    12361 cgcgtcggcg gacgcagcct gccggagctg cgggcggagt cggaatcact cgaagccgag
    12421 accgcgacgg cgcggcggcg cgtggagcga ctgaccgagc tgctccgctc ggaggcgacc
    12481 agcgccgccg agctggaacg g
//
LOCUS       DASRIQ010000369        12266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2767416, whole genome shotgun sequence.
ACCESSION   DASRIQ010000369 DASRIQ010000000
VERSION     DASRIQ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12266
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767416"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFD06_08835"
     CDS             <1..426
                     /locus_tag="VFD06_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN03676.1"
                     /translation="DRLPHAARRDAVLGCTGGRGADVVIEAAGVPAAVAEGTTLCRDG
                     GVLVVAGHYTDGGEMALNPHAQLNRPHLEVRGCWGSDASHFEGAIRTMARHQGRVPWG
                     EMVTRWFPIEESGAALQAVADRSVLKSGIRLSKGSAPRA"
     gene            complement(442..516)
                     /locus_tag="VFD06_08840"
     tRNA            complement(442..516)
                     /locus_tag="VFD06_08840"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(481..483),aa:Glu,seq:ctc)
     gene            complement(526..599)
                     /locus_tag="VFD06_08845"
     tRNA            complement(526..599)
                     /locus_tag="VFD06_08845"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(565..567),aa:Gln,seq:ctg)
     gene            complement(636..2144)
                     /gene="gltX"
                     /locus_tag="VFD06_08850"
     CDS             complement(636..2144)
                     /gene="gltX"
                     /locus_tag="VFD06_08850"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZN03677.1"
                     /translation="MTPNDSPPRGPRVRFAPSPTGFLHVGGGRTALFNWLFARRHQGV
                     FILRIEDTDLERSNEASVRTILEGMEWLGLDWDEGPFFQSRGVEAHRALARRLESEGK
                     AYPCFCAIEEIEARRRAAEAAGGAWRYDRTCLRLSAAERDERRARNATSALRFRVPDG
                     VTAWDDRVHGETSFDNGVIEDLVLLRSDGSPTYNLSCVADDIAMKVTDVIRGDDHISN
                     TPKQILLYRALGHEPPTFAHLPLILGPDKKRLSKRHGAVSVTEYRDTGILPAAMRNFL
                     ALLGWSPGDGRERLSREEMVEAFDIGAVNRKGAVFDEQKLEWLNSQYIQDLPAERLAE
                     AVRPYLEGAGMLSADLDPGGARRDWYLRVLETLKARSRRLTDFPRDARPFLGEEYDIQ
                     PEAVAKYLTAEKSGGPGAVAARLRALAGALRALEPFDETASEAALRDLASGRGDSAAL
                     YIHPMRVSLLGTAVGPSAFAVLVLIGKERATARLERVAARLESGGFQAPEGG"
     gene            2263..3798
                     /locus_tag="VFD06_08855"
     CDS             2263..3798
                     /locus_tag="VFD06_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN03678.1"
                     /translation="MPPWPNPRRHAPVDLTGRLGVFIFLAAASMAASLSGGSLRQTPP
                     AAPGFPIPIPRTTDYRGLWGSTLAVDLDRDGRSELLASVPSGVLFLIDRGGTAVPGWP
                     RSVSDLPAPASLVGQPGVGDLDGDGTDEVVACVNAGAAPRRAFLVAWSRDGNRVTGWP
                     VEIPTLDPYAGCSPGGTLVADLDADGRAEVAQAVSPSEIWLYDGDGRPLSGWPFRPPL
                     REWGIAPRINARLATADLDGDGRREILAVESGLGPLLHALTLQGGEAAFFPRPLDEIV
                     DTQAPAAGDLDADGVDEVVQATLPVSNEASGEPAPMIPGALHSLRRDGVEAEGWPVPL
                     SSGAMWGAMLLDLDGDGRPEVLQGDGDTLHAFDATGKTLQGFPLTMRRLFTRATVRLD
                     SRWVAGDLDSDRSPDFLRALGRMDGDVVELRVAAIRSRTGSPVAGTPWTIEGLFPASD
                     PVLVDLTGDGAPEVAMLAAEGGGGGWRLMAWDVAAGRNGRGAGIRTDRQRVGVSLPGP
                     IPR"
     gene            complement(3770..4864)
                     /locus_tag="VFD06_08860"
     CDS             complement(3770..4864)
                     /locus_tag="VFD06_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03679.1"
                     /translation="MRRTVGFLALLCAVASSAAQDAPVMPPAGMTGPAPQTPGKKSPP
                     SPAPKKKDAPAPAPAAAVTLTPRAWSRSAYSFEGRLESGIQDVSFDTPPAYKESFAFW
                     ANRMKGTSRTEQIQYVTSTRDPEESGRIPFRRQVSRYMIDLTEQGQIKTMGGKLNRDV
                     QLLAWEGRFDPKGRIVEVKRVAGPEDATEVDRLAFPLLEQILPPLDAPQSVKVGESLT
                     FESTLPLPSRLTINGLEDAAARLTRVLTLKEMRGRQAVFAVQASYALDPSTPPKVPRT
                     TCTIGGGGDGEAIFDLDAGFFTSARLGTKMLIDIEAPLRPLPDQAEGTDPGTGKSHMV
                     LVLNMSGKQSATQLFGGDGSGVSEGSDRAD"
     gene            complement(4867..6609)
                     /locus_tag="VFD06_08865"
     CDS             complement(4867..6609)
                     /locus_tag="VFD06_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03680.1"
                     /translation="MSQPFWSRQELERYLRYEDPEVRYWAAERLARLYPGESAGALAP
                     FLFDDHELTPELVASQLGRHGGSEHAAPLARAAERLRGLAAARALEALVRLKAPKALD
                     LVRDAAARRDLDEECWAAILEALSERNDPPARAAMQEVLKRRADWFGSPPILAAALAH
                     ARPGEYRPLLQAWLRSLQWRGTGRASDSEATGEAFRVLMDHLQVDDCGWCFRTTLSGR
                     IDLARTLKAIESAYDCTLAPALEPAIAAGLGKALEEGEYDTSAARLAEAVRSLVRAAG
                     KAPGDDLPGRIEEAASFWADPEVARAVEGLGPHLREWVIGFLLSAVIKMARYRNYELE
                     VRRAATDLEALLPLLEVETSALLEALPEAIRNTVDAAGPAGSPGRSEARRAVEERCLA
                     LLAMRGPFFPQVMALETLGALGSAGVISEILDALAEDNSYLYEAAEHALTSIGEAVVE
                     PARARLEAGSLGADAAHSLLMVLCELGTPKALKLVLDHFDAFAEAAGAADAARWMSLF
                     GARELIDPLRRILSQDMAQVGQALLLLGAVHNVRIPEETAIRRAIDDYWKEQPEESDG
                     GDTGPDDGSGKYVM"
     gene            6754..7881
                     /locus_tag="VFD06_08870"
     CDS             6754..7881
                     /locus_tag="VFD06_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN03681.1"
                     /translation="MALSGASLLTAAFLLEGGARLLLPQPERVTIHAVPDLDDRLARE
                     NRDPKKLAYRGAVNALFIKTPTGRRLRASTEVTIENHYLSHRTIVLRTNSLGFRGPEL
                     GAKTRARVLFLGDSITMAHYLPDEETFVRLVGAASERDGGTPLETINAGVGGVGLENE
                     LALLRETGLRAEPDIVVLEFYLNDVQPSPGVGLLWLPSFIDWSIAARHVANTLPRLFA
                     RDDREIGDAEMAGWLTEIRRRAPASGGDPGRDPGAFNALVQENYRDWGSAWSDGVWDR
                     LDPLLSELKRLAEEHRFRLRIVAFPVEPQVNASFVNDWPQRRLAATAARLDIPMLDLL
                     PPLRAARARGGEPLFYDVCHHTPDGSAVVADAILDFLRRNA"
     gene            complement(8002..9000)
                     /locus_tag="VFD06_08875"
     CDS             complement(8002..9000)
                     /locus_tag="VFD06_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN03682.1"
                     /translation="MSDRDAIAPLIPWYAAGTLGAAESAEVEAHLASCDDCRGLLALA
                     RGFRRLAPQVADPDLFEHVQAQRLAEFAENPSALEPEARRFITTHIRSCSPCAEALEI
                     LEDLGSVPAAGSPMGTQATPGSGTLERARRAWEEIWRRLSRSVLRPAPAFAYLAALIV
                     LVVALPLRNADRGVEGEPPPPIPAPAPAPVPSPAPPPRPPFLLLPPPIDLPEEVVFRD
                     GTPAPKPMEVRLPAGTAPVPLVLRTSLGSDDLGDREARYRVTITQGDRTLFEREIAGA
                     DFDRRARLTLFLDPSEVAAGIPCRATIVRVKPGDPAHGEEVYRRSFVLVQGPAPIR"
     gene            complement(9000..9557)
                     /locus_tag="VFD06_08880"
     CDS             complement(9000..9557)
                     /locus_tag="VFD06_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN03683.1"
                     /translation="MSEASDDDILTGFLAGDDEAVHHVQGWVDGVVHLGRWRFEDPES
                     VRQEIVMRLLAIVRSGRYGKRSTFKTFVFAVAKYTCVDLFRRERLRPRVETGERAPMP
                     QPIGQPHRTLEEREDLELLRYILQGLPEECRQLWRWAYGDGIDANEIGRRLGVNPGTV
                     RVRVHRCLQKARAFALQHSGGPGLI"
     gene            9851..10945
                     /locus_tag="VFD06_08885"
     CDS             9851..10945
                     /locus_tag="VFD06_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03684.1"
                     /translation="MRGTAGRLAVTTLAMAAALLASPAAAVQYDLLVDVPTTLGGVTY
                     TQNQIVRYHDGAYVLEASFPEEVPLAALLRTPEGKWLLSPAAVVPLDDGALEPRDLIL
                     FDPLDEDASVLVLDGSAAGVPAYAAIDAVLWDRVTDQGALSFDVPVNLGGVEYGPADL
                     VALGAGGFSLFWSAAAAGVPPYANIVGADQDGAGRLVLSFDVPVTLGGLTFHPGQLVQ
                     WTSGGGFTLYASDPAWPASAVLGDFGFLPAAGGLPDGGAGSTALTVVPAAGGQITLSW
                     GPSCAATDSDYAVYEGTLGAPFTSHVPVTCSTGGATSWTFTPGAGNRYYYVVPRNAVA
                     EGSYGHVSVGAPRPPSAAACLPQEVAPSCG"
     gene            11016..12086
                     /locus_tag="VFD06_08890"
     CDS             11016..12086
                     /locus_tag="VFD06_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZN03685.1"
                     /translation="MNRKLLVALALLTAPLGLATAAEGRIPLLSLPGPPYVITQPGHY
                     YLTRDIGGTPAAVVRIQSDGVTLDLNGFTVSGPTSFVDTTIDINLSSPANLRGITIRN
                     GRTFGGAYGVRAFGTKGRIRVEKVEISGSRDGISLDADHAEVIDCHVHDLVTVGGAPG
                     GGIAVLAYGGRIIDNMVQNTPGTGISALGLRGGEIRRNIVRNFGAAMPGAWGIKIDDD
                     AFEPGNTNGAVLADNVVSGLPGGTDDYGIWVRGTDHTITGNAASYNGRDGILIQGPYN
                     HIERNVTNRNGANGLGFAILYPHNFVVANQSQGNTGCGINVNGASTIYRDNLFAGNTG
                     GNVCTGPGINAGGNYCNSALCP"
BASE COUNT         1737 a         4430 c         4297 g         1802 t
ORIGIN      
        1 gatcggctgc cgcacgccgc ccggcgcgac gccgttctcg ggtgcaccgg gggacgcggg
       61 gccgatgtcg tcatcgaggc tgcgggggtc cccgcggcgg tggcggaggg gacgacgctg
      121 tgccgcgacg gcggggtcct cgtggtcgcc gggcactaca ccgacggcgg cgagatggcg
      181 ctcaacccgc acgcgcagct caaccgtccg cacctcgagg tgcgcggctg ctgggggagc
      241 gacgcctcac acttcgaggg ggcgatccgg acgatggccc gtcaccaggg acgcgttccg
      301 tggggagaga tggtgacccg ctggtttccg atcgaggaga gcggcgccgc ccttcaggcg
      361 gtggccgatc gcagcgtgct caagtccggc atccggctgt cgaaaggttc agcgccgcgg
      421 gcgtgaggtg gaaggcctgg gtggtagcgc tacggggatt cgaaccccgg tctgatggct
      481 gagaaccatc tgtcctaacc cctagacgat agcgccatgc cggcctggtt ccgggacagg
      541 gattcgaacc ccgattcctt ggtccagagc caagtgtcct gccgttagac gatcccggaa
      601 cggagcgccg attttagtga accgaccggg aggcgtcaac cgccttccgg cgcttggaat
      661 cctcccgatt cgagacgcgc ggcgacacgt tcgaggcgcg ccgtcgctcg ctccttcccg
      721 atgaggacga ggacggcgaa ggcgctcggt ccgaccgccg tgccgagaag cgatacgcgc
      781 attgggtgga tgtagagggc cgccgagtcg ccccgtcccg acgccagatc gcgcagcgcc
      841 gcttcggacg ccgtttcatc gaacggctcg agcgctcgca gggcgcctgc gagcgctcgg
      901 agccgcgccg ccacggcgcc cgggcctccg ctcttttcgg ccgtcaggta cttcgcgacc
      961 gcctccggct ggatgtcgta ctcctctccc aggaacggcc gggcgtcgcg cgggaagtcg
     1021 gtcaggcgcc ggctccgcgc cttgagcgtc tcgaggacgc gcaggtacca gtcgcgccgc
     1081 gccccccccg gatcgagatc ggccgacagc atgccggccc cctcgagata aggacggacc
     1141 gcctccgcca ggcgttccgc cgggaggtcc tggatgtact ggctgttgag ccattcgagc
     1201 ttctgctcgt cgaacaccgc ccccttgcgg ttcacggcgc cgatgtcgaa cgcctcgacc
     1261 atctcctcgc gcgacaggcg ctcgcgcccg tctcccggcg accagccgag cagcgccagg
     1321 aagttgcgca tcgccgccgg caggatgccg gtgtcgcggt actcggtcac cgagaccgcg
     1381 ccgtgccgct tggagagccg tttcttgtcc ggtccgagga tgagcggcag gtgggcgaag
     1441 gtcggcggct cgtgtccgag cgcgcggtac agcaggatct gcttcggcgt gttgctgatg
     1501 tgatcgtcac cgcggatgac gtcggtcacc ttcatggcga tgtcgtcggc gacgcaggag
     1561 aggttgtaag tcggcgagcc gtcggagcgc agcagcacca ggtcctcgat gactccgttg
     1621 tcgaacgacg tctctccgtg cacccggtcg tcccaggcgg tcacgccgtc cggcacgcgg
     1681 aagcgcaggg ccgacgtcgc gttccgcgcc cgccgctcgt cccgctcggc ggcgctgagc
     1741 cgcaggcagg tccgatcgta gcgccaggcc ccgcccgccg cctccgcggc gcggcggcgc
     1801 gcctcgatct cctcgatcgc gcagaagcag gggtacgcct tcccctccga ctcgaggcgc
     1861 cgggccagcg cccggtgcgc ctcgacgccg cgcgactgga agaacggccc ttcgtcccag
     1921 tcgagcccca accactccat cccctcgagg atcgtccgca cgctcgcctc gttgctgcgc
     1981 tcgaggtcgg tgtcctcgat cctcaggatg aaaacgccct ggtgccggcg cgcgaagagc
     2041 cagttgaaca gggcggtccg ccccccgccg acgtgcagga agccggtcgg gctcggggcg
     2101 aagcggacac ggggccccct cgggggggag tcgttcgggg tcatgggagg gggattaaag
     2161 ccgaagcgcc gtcaggccac aagccgaaac gacattgcac ccgcccggcg ggcacagtat
     2221 aagggtggca tcggaagctg gaacgaggca gggagggtgc ttatgccgcc atggcccaat
     2281 ccccgtcgcc atgcccccgt cgacctgacg ggacgcctgg gcgtcttcat cttcctcgcc
     2341 gccgcgtcga tggccgcgag cctctccggc ggttcgctgc ggcagacgcc gccggccgct
     2401 ccgggattcc cgattccgat cccgcgcacc acggattacc ggggcctgtg gggctcgacc
     2461 ctcgccgtcg acctcgatcg cgacgggcgg tcggagctgc tcgcctcggt cccttcgggg
     2521 gtgctcttcc tgatcgaccg gggggggacc gccgttccgg gctggcctcg ctccgtctcc
     2581 gacctcccgg ctccggcttc tctggtcggg cagccgggcg tgggggatct cgacggcgat
     2641 ggaacggacg aagtcgtcgc ctgcgtcaac gccggcgctg cgccgcggcg ggcattcctc
     2701 gtcgcgtggt cccgcgatgg gaatcgcgtg accggctggc cggtggagat cccgaccctc
     2761 gatccgtacg ccggctgctc gccgggaggg acgctcgtcg ccgatctcga cgcggacggg
     2821 cgggcggagg tggcgcaggc ggtcagtccc tcggagatct ggctgtacga cggcgacggg
     2881 cgtccgctgt cgggctggcc gttccgtcct ccgctgcggg aatggggcat cgcgccccga
     2941 atcaacgcgc ggctcgccac cgccgatctc gacggcgacg gccggcggga gatcctcgcc
     3001 gtcgaatccg gcctcggtcc gctgctgcac gctctgacgc tgcagggggg cgaggcggcg
     3061 ttcttcccga ggcccctcga tgagatcgtg gacacgcagg ccccggcggc gggggatctc
     3121 gacgcggacg gcgtcgacga ggtggtgcag gcgaccctgc cggtctcgaa cgaggcctcg
     3181 ggcgagccgg cgccgatgat cccgggggcg ctccactcgc tgcggcgcga cggcgtcgaa
     3241 gcggaagggt ggccggtccc cctctcgtcc ggggcgatgt ggggggcgat gctgctcgac
     3301 ctcgacggcg acggccggcc ggaggtcctg cagggggacg gggacacgct ccacgccttc
     3361 gacgccacgg ggaagacgct gcagggattc ccgctgacga tgcggcggct gttcacgcgc
     3421 gccacggtgc gcctcgactc gcgctgggtg gcgggggacc tcgactccga tcgctccccc
     3481 gatttcctgc gcgccctcgg gcggatggac ggggacgtcg tcgaactgcg ggtggcggcg
     3541 atccgcagcc gcaccggatc gcccgtggcg ggaacgccgt ggacgatcga ggggctcttc
     3601 cccgcctccg acccggtcct cgtcgatctg accggcgacg gcgcgccgga ggtggcgatg
     3661 ctcgccgcgg aagggggcgg cggcggatgg cgcctgatgg cgtgggacgt ggctgccggc
     3721 cgcaacggcc ggggcgccgg catccgcacc gatcgtcagc gcgtcggcgt cagtctgccc
     3781 ggtccgatcc ctcgctgacc ccggagccgt cgccgccgaa cagctgcgtc gccgactgct
     3841 tcccggacat attgaggacg agcaccatgt ggctcttccc ggtccccggg tcggttcctt
     3901 ccgcctggtc ggggaggggc cgcagcggcg cttcgatgtc gatgagcatc ttcgtcccca
     3961 gccgcgcgct cgtgaagaac ccggcgtcga gatcgaagat cgcctccccg tcgccgccgc
     4021 cgccgatggt gcaggtcgtg cgcggcacct tcgggggggt cgaaggatcg agagcatagg
     4081 acgcctgcac cgcgaacacc gcctggcggc cgcgcatctc cttgagggtc aggacgcggg
     4141 tcaggcgcgc cgccgcgtcc tcgagcccgt tgatcgtcaa acgcgacggc agcggcaggg
     4201 tggactcgaa ggtgagcgac tccccgacct tgaccgattg cggcgcgtcg agcggcggga
     4261 ggatttgctc gagcagcggg aacgcgaggc gatcgacctc ggtcgcatcc tccggaccgg
     4321 cgacccgctt cacctcgacg atgcgccctt tcgggtcgaa ccggccctcc caggccagga
     4381 gctgaacgtc ccggttcagc ttccccccca tcgtcttgat ctgtccttgc tcggtcaggt
     4441 cgatcatgta gcgcgacacc tgccggcgga acgggatccg ccccgactcc tcggggtccc
     4501 gggtggacgt gacgtactgg atctgctccg tccgcgacgt ccccttcatc cggttcgccc
     4561 agaaggcgaa gctctccttg taggcgggcg gggtgtcgaa gctgacgtcc tggatccccg
     4621 actccaaccg accttcgaag gagtaggcgg accgggacca ggcgcgcgga gtgagcgtca
     4681 ccgccgcggc cggcgccgga gcaggggcgt ccttcttttt cggcgccggg ctcggggggg
     4741 acttctttcc cggagtctgg ggagccggcc ccgtcatgcc cgcgggaggc atgaccggcg
     4801 cgtcctgcgc cgcggacgac gcgacggcgc agagcagggc gaggaatccg acggtccggc
     4861 gcacgctcac atcacgtatt tgccggaacc gtcgtccggg ccggtgtcgc cgccgtcgct
     4921 ctcctccggc tgctccttcc agtagtcgtc gatggcacgg cggatcgccg tctcctccgg
     4981 gatgcggacg ttgtgcaccg cccccaggag gaggagcgcc tgcccgactt gcgccatgtc
     5041 ctgcgacagg atgcggcgca gcgggtcgat cagctcgcgc gcgccgaaca gcgacatcca
     5101 tcgggccgcg tcggccgccc cggccgcctc ggcgaaggca tcgaagtgat cgagcacgag
     5161 cttgagcgcc ttcggcgtcc ccaactcgca cagcaccatg agcaggctgt gggcggcgtc
     5221 ggccccgagg ctgccggcct cgaggcgcgc ccgcgccggc tcgaccaccg cctcgccgat
     5281 cgacgtcagg gcgtgctccg ccgcttcgta caggtaggag ttgtcctccg ccagcgcgtc
     5341 gaggatctcg ctgatcacgc cggcgctgcc cagggcgccg agcgtctcga gcgccatcac
     5401 ctgcgggaag aacggcccgc gcatcgcgag gagcgccagg cagcgctcct cgacggcgcg
     5461 gcgcgcctcc gaacggcccg gcgatccggc cggcccggcg gcgtccacgg tgttgcggat
     5521 cgcctccggc agcgcctcga ggagcgcgct cgtctccact tcgagcaacg gcaggagcgc
     5581 ctcgagatcg gtggcggcgc gccgcacctc cagctcgtag ttgcggtagc gggccatctt
     5641 gatcaccgcg gagagcagga agccgatgac ccactcgcgc aggtgcggcc ccagcccttc
     5701 caccgctcgc gccacctcgg gatcggccca gaaggacgcg gcctcctcga tccggcccgg
     5761 gaggtcgtcc cccggcgcct tgccggcggc gcgcaccagg ctccgcacgg cctcggccag
     5821 gcgggcggcg ctcgtgtcgt actccccttc ctcgagcgcc ttccccagcc ccgcagcgat
     5881 ggccggctcg agcgccgggg cgagcgtgca gtcgtaggcc gactcaatcg ccttgagcgt
     5941 gcgcgccagg tcgatccgtc cgctcagcgt ggtgcggaag caccagccgc aatcgtccac
     6001 ctggaggtga tccatgagga cgcggaaggc ctccccggtc gcctcgctgt cggacgcccg
     6061 cccggtgccg cgccactgca gggatcgcag ccacgcctgc aggagcggcc ggtactcccc
     6121 cggccgcgcg tgggcgaggg cggcggccag gatcggcggc gacccgaacc agtcggcccg
     6181 gcgcttcagg acctcctgca tggccgcccg cgccggcggg tcgttgcgct ccgacagggc
     6241 ctcgaggatc gcggcccagc actcctcgtc gagatcacgg cgggcggcag cgtcgcgcac
     6301 gaggtcgagg gccttgggcg ccttgaggcg gacgagcgcc tcgagggccc gcgccgccgc
     6361 aaggccgcgg agccgctcgg cggcccgcgc cagaggggcg gcgtgctccg aaccaccgtg
     6421 ccgtcccagt tgagaggcca cgagctccgg cgtcagctcg tggtcgtcga acaggaacgg
     6481 ggcaagagcc cctgccgatt cgccgggata cagacgcgcc agccgctcgg cggcccagta
     6541 ccgcacttcg ggatcctcgt agcgcaggta acgctcgagc tcctggcgcg accagaaggg
     6601 ctggctcatg gggcgtgggg tctccgtgga ccgggcatta tagccgatgc tccggagcct
     6661 cccctccacg aggtcaaccc tccggccgcc cgccgcgtta cacacggcat gcccggcccg
     6721 atcgaccgcg ggaagttcgg gactcaccac ggattggcgc tgtccggtgc ctccctgctc
     6781 acggcggcgt tcctcctgga agggggggcg cggctcctcc tgccgcagcc cgagcgcgtc
     6841 acgatccacg ccgtccccga tctcgacgac cgcctggcgc gcgagaaccg cgacccgaag
     6901 aagctcgcct atcgcggcgc ggtgaacgcc ctcttcatca agacgcccac cgggcgccgg
     6961 ctgcgggcca gcaccgaagt caccatcgag aaccattacc tgagccaccg caccatcgtg
     7021 ctgcgcacga actcgctcgg attccgcggc ccggagctgg gcgcgaagac ccgcgcgcgc
     7081 gtcctgttcc tgggggactc gatcaccatg gcgcactacc tgccggacga ggagaccttc
     7141 gtccggctcg tgggcgcggc gtcggagcgg gacgggggga cgccgctcga aaccatcaat
     7201 gccggcgtcg gcggcgtcgg gctcgagaac gagctggccc tgctccgcga gaccggcctg
     7261 cgcgccgaac ccgacatcgt cgtcctggag ttctacctga acgacgtgca gccctctccc
     7321 ggcgtcgggc tcctgtggtt gccgtccttc atcgactgga gcatcgccgc ccgccacgtc
     7381 gcaaacaccc tgccgcgcct cttcgcccgc gatgatcggg agatcggcga cgcggaaatg
     7441 gcgggttggc tcaccgagat ccgccggcgc gccccagcgt cgggcggcga cccgggccgc
     7501 gaccccggtg cgttcaacgc cctggtgcag gagaactacc gcgactgggg gagcgcctgg
     7561 agcgacggcg tctgggatcg gctcgatccg ctcctgtccg agctcaagcg gctggccgaa
     7621 gagcaccggt tccgcctccg catcgtggcg ttcccggtcg aacctcaagt gaatgcctcc
     7681 ttcgtcaacg actggccgca gcggcgcctc gcggccaccg ccgcgaggct cgacatcccg
     7741 atgctcgacc tcctgccgcc cctgcgcgcg gcgcgtgcgc gcggcggcga gccgctcttc
     7801 tacgacgtct gccatcacac gccagacgga agcgccgtcg tcgcggacgc gatcctcgac
     7861 ttcctccgcc ggaacgcctg acacgccttc gtccgtttca atttcggacg gtcgtcccac
     7921 gggcggacac tgatacaagg atgagaaccg ccccgcgggc gcgatgccgc accccggctt
     7981 tttctggtgg ccatgcgaca gtcagcggat gggagcggga ccctgaacga gcacgaacga
     8041 gcggcggtac acctcctcgc cgtgtgccgg gtcccccggc ttcacgcgga cgatggtggc
     8101 gcgacaggga atcccggcgg cgacttccga cgggtcgaga aagagggtca gccgggcgcg
     8161 ccgatcgaag tcggctccgg cgatttcccg ctcgaacagg gtgcgatccc cctgcgtgat
     8221 cgtcacccgg tagcgggcct ctctgtcacc gagatcgtcg gaccccaggc tcgtcctcag
     8281 cacgagcggc accggcgcgg tcccggccgg aaggcggacc tccatcggct tcggtgcggg
     8341 cgtgccgtcg cggaacacca cctcctcggg gagatcgatg ggcgggggaa gcagcaggaa
     8401 cggcgggcgg ggcggcggcg cgggcgaggg gaccggagcc ggcgccggtg cgggaatggg
     8461 aggcggtggc tccccctcca ccccgcgatc ggcgttccgc agcggcagcg ccacgaccag
     8521 gacgatgagc gccgcgagat aggcgaacgc cggcgccgga cggagcacgc tccggctcaa
     8581 gcgccgccag atctcctccc atgcccggcg agcacgctca agcgtgccgg agcccggcgt
     8641 cgcctgcgtg cccatgggtg accctgcggc cggcacgctc cccagatcct ccaggatctc
     8701 cagcgcctcg gcgcagggag agcaggaccg gatgtgcgtc gtgatgaagc gccgcgcttc
     8761 cggctcgagc gccgacgggt tctcggcgaa ttccgccagc cgctgcgcct gcacgtgctc
     8821 gaagagatcc ggatcggcga cctgcggcgc cagccgtctg aagccgcgcg ccagcgccag
     8881 gagtccgcga cagtcgtcgc acgaggcgag atgcgcctcg acctccgcgc tctcggcggc
     8941 gccgagggtc cccgccgcgt accagggaat cagcggggcg atcgcatcgc ggtcgctcat
     9001 cagatcaacc ccggtccgcc ggagtgctgc agcgcgaacg cgcgcgcctt ctgcaggcag
     9061 cggtggaccc gcactcgcac cgtaccgggg ttgaccccga ggcggcggcc gatctcattg
     9121 gcatcgatcc cgtcgccgta ggcccacctc cacagctgcc ggcattcctc gggaagcccc
     9181 tgcaggatgt agcgcaggag ctcgagatcc tcccgctcct cgagcgtgcg gtgcggctgg
     9241 ccgatcggct gcggcatcgg ggcccgctcg ccggtttcga cgcggggccg caggcgctca
     9301 cgccggaaga gatcgacgca ggtgtatttc gccaccgcaa agacgaaggt cttgaaggtc
     9361 gatcgcttcc cgtaccgccc ggatcggacg atggcgagga gacgcatgac gatctcctgc
     9421 cggaccgatt cggggtcctc gaaacgccag cgtcccaggt ggaccacacc gtccacccag
     9481 ccctgcacgt ggtgcaccgc ctcgtcgtcg ccggcgagga agccggtcag gatgtcgtcg
     9541 tccgacgcct cgctcatggc atccccttcc ggcccgccgc gcttcatcgt cgggtgaggg
     9601 ccgagtctac acctccggtt atcgcagcat caccagcatc cggatcgagc cggcgccgcc
     9661 gccgagcgac tcgagcgccc catcgtcgtc ctccaagctc gatgtcgaac cccttagccg
     9721 gacgacgacg cgaagcgtta cgccgcacgg tcgaagcgca tcgcccgtaa cgccggtccg
     9781 gctcgtccta caaagggagg agcaggacat gaacagggag ggcgaagcga tgcgaagcac
     9841 gaggcagtgc atgcgcggaa cggcgggccg gctggcagtc acgacgctcg ccatggcggc
     9901 ggcgctcctc gcctcccccg cggccgcggt tcagtacgac ctgctcgtgg acgtcccgac
     9961 gactctcggc ggcgtcacct acacccagaa tcagatcgtg cgctaccacg acggcgccta
    10021 cgtcctcgag gcgagcttcc cggaggaggt gccgctcgcc gccctgctgc gcacacccga
    10081 ggggaaatgg ctcctgtcgc cggcggccgt ggttccgctc gacgacggcg ccctcgagcc
    10141 gcgcgacctc atcctcttcg acccgctgga cgaggacgca tcggtcctgg tgctggacgg
    10201 gagcgccgcc ggcgtcccgg cgtacgccgc catcgacgcg gtgctgtggg accgggtcac
    10261 cgaccagggg gcgctgtcgt tcgacgtgcc ggtgaatctg ggcggggtgg agtacggccc
    10321 cgcggatctc gtggcgttgg gcgccggcgg cttttccctg ttctggagcg ccgcggccgc
    10381 gggtgtgccg ccctacgcca acatcgtcgg ggccgaccag gacggcgccg ggcggctggt
    10441 cctgtcgttc gacgtgccgg tcacgctcgg gggcctgact tttcaccccg gccagctggt
    10501 gcagtggacc tccggtggcg gcttcacact gtacgcgtcc gatcccgcct ggccggcgtc
    10561 ggcggtgctc ggcgacttcg gcttcctgcc ggccgcgggg gggctgcccg acggcggcgc
    10621 cggcagcacc gctctcaccg tcgtgccggc cgccgggggc cagatcaccc tctcctgggg
    10681 tccctcctgc gccgccaccg attccgacta cgccgtctac gaggggacgc tcggtgcgcc
    10741 gttcacctca cacgtcccgg tcacgtgcag cactggaggc gccacctcct ggacgttcac
    10801 tccgggagcc ggcaaccgct actactacgt ggtgccgcgc aatgcggtcg ccgaggggtc
    10861 gtacggccat gtcagcgtcg gcgcgccgcg accgccgtcg gccgccgcgt gcctgcctca
    10921 ggaggtggcg ccgtcatgtg gctgatgcgg cgttcgtgtt ctcgggtctg cgacaccgtc
    10981 tcgaatcgcc ccaatcccgg ggccggaggt ccgtcatgaa caggaagctt ctcgtcgccc
    11041 tggctttgtt gaccgctccg ttgggtctcg cgaccgccgc cgagggtcgc atcccgttgc
    11101 tgtcgctccc ggggccgccc tacgtcatca cccagcccgg gcactactat ctgacgcgcg
    11161 acatcggagg aacccccgca gccgtcgtgc gcatccagtc cgacggcgtc acgctggatt
    11221 tgaacgggtt cacggtcagc ggccccacca gcttcgtcga cacgacgatc gacatcaacc
    11281 tctcatcgcc ggccaacctg cggggcatca cgatccgcaa cggccgcacg ttcgggggag
    11341 cctacggggt gcgcgcgttc ggaacgaaag gacgcatccg tgtcgagaag gtcgagatct
    11401 ccggctcccg cgacgggatc tccctcgacg cggatcatgc cgaggtgatc gactgtcacg
    11461 tgcacgacct cgtcacggtc gggggcgccc cggggggcgg catcgcagtg ttggcctatg
    11521 gcgggcgcat catcgacaac atggtccaga acacgccggg gaccggcatc tccgcactcg
    11581 gtctgcgggg cggtgaaatc cggcgcaata tcgtgcgcaa cttcggtgca gcgatgccgg
    11641 gcgcctgggg catcaagatc gacgacgatg ccttcgaacc ggggaacacc aacggcgccg
    11701 tcctcgccga caacgtcgtc agcgggctgc cgggcggcac cgacgactac ggcatctggg
    11761 tccggggcac cgaccacacg atcacgggga acgcggcctc ctacaacggc cgtgacggga
    11821 tcctcatcca gggtccctat aaccacatcg agcggaatgt cacgaaccgc aatggtgcca
    11881 acggcctggg attcgcgatt ctttacccgc acaatttcgt ggtcgcgaat cagtcgcagg
    11941 ggaacaccgg ctgcggcatc aacgtgaacg gcgcttcgac catctaccgg gacaacctct
    12001 tcgccggcaa caccggcggc aacgtctgca ccgggccggg catcaacgcc ggcgggaact
    12061 attgcaactc ggcactctgt ccatgaccgg gagccgcagg gggcgccccg catcgtcgat
    12121 gcgcgagggc cggacacggt agacttggcg ccagattgga gccgctgcat gactcacccg
    12181 tggcgctcgg tcccaacgct cggctgcctg ctcctgatct cgtgggtggc gatgccgtcc
    12241 gcagaaatga gtcaggctgc caaccc
//
LOCUS       DASRIQ010000370         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2222818, whole genome shotgun sequence.
ACCESSION   DASRIQ010000370 DASRIQ010000000
VERSION     DASRIQ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222818"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFD06_08895"
     CDS             <1..736
                     /locus_tag="VFD06_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN03686.1"
                     /translation="FGWFSWFAAGILYVGQLKADGALPGPVVINFSIQAGGGSAILAS
                     AIDYATSQGVIFVTIAGNFGPDAGTISFPGRRPQSITAGAAGWLREFCEPFCAAPWFF
                     ADVPENDATQVYVAGFSGREAIPPPSPSAIDVLAPGSFVFGEWVFGPGFSEGREVAFD
                     AVDNFIFGTSFAAPHVAGIVAQMLEKNPGLDQEAVEGILRGSALPIPPSGDLPTPVQI
                     VPGWGANATGAGLARGAAAIVATPDP"
     gene            complement(740..1756)
                     /locus_tag="VFD06_08900"
     CDS             complement(740..1756)
                     /locus_tag="VFD06_08900"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HZN03687.1"
                     /translation="MWGLLAAVSMGRAALADATVVPAAQDATLIEDPQGSLATGSGPA
                     IFAGRIASTLQALRRGLVRFDVASAVPAGSTIDAVRLRLNLSMAASGPVTVRLHRVTQ
                     SWGEGASSSFGGNGVPSQPGDATWIHRFHDDVFWTQPGGDFDPVARGEATVDQTGPYA
                     WDSTPEMVEDVQSWLDDPTANFGWMLIGDEDRLQTARRFDSRENPDPLVRPALEILYT
                     TPCLPDPAGPGFWRQACGESLDEAVSTCAAATFTALGLPEIDSCSAVLAPAPPSCNAR
                     AARKLAVLVLNLCANRLQTSCPVSQDDSVCRAEDVGGQIEAMAALLAAGDCRRAAACG
                     GLLE"
     gene            1949..>2196
                     /locus_tag="VFD06_08905"
     CDS             1949..>2196
                     /locus_tag="VFD06_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03688.1"
                     /translation="MTETSSGDDALAGFRRRHPEADRWATGCGVIRHTDETQARIFSM
                     AAALGAGRPAPEGFFARLEAADRVVSAAMWLVVHETYA"
BASE COUNT          312 a          777 c          783 g          324 t
ORIGIN      
        1 cttcggctgg ttctcctggt tcgcggccgg catcctttac gtcggtcagc tcaaggcgga
       61 cggcgcgctc ccggggccgg tggtgatcaa cttcagcatc caggcaggag gcggctccgc
      121 catcctcgcc agcgccatcg attacgcgac gagccagggg gtgatcttcg tcacgatcgc
      181 cgggaacttc gggccggacg ccggcaccat ttcgtttccg gggcgacgcc cgcagtcgat
      241 caccgccggc gccgccggct ggctgcggga attctgtgag ccgttctgcg cggccccgtg
      301 gttcttcgcg gacgtgccgg agaacgacgc cacccaggtc tacgtcgccg gcttctccgg
      361 gcgcgaggcg atcccccctc cgtcgccgag cgccatcgac gtcctggcgc cggggtcgtt
      421 cgtcttcggc gagtgggttt tcggtcccgg gttctcggag gggcgagagg tcgcgttcga
      481 cgccgtggac aacttcatct ttggcacctc gttcgccgcg ccgcacgtcg cggggatcgt
      541 ggcgcagatg ctcgagaaaa accccggcct cgatcaggag gcggtggagg ggatcctgcg
      601 cggcagcgca ctgccgattc ccccttcggg ggatctgccc acgccggtgc agatcgttcc
      661 cggctggggc gcgaacgcca ccggcgccgg gctggcgcgc ggcgccgccg ccatcgtggc
      721 gacgccggac ccgtaggcct tactccaaca gtccgccgca ggcggcggcg cggcggcaat
      781 ccccggcggc gaggagcgcg gccatcgcct cgatctggcc gccgacgtct tcggcgcggc
      841 agaccgaatc gtcctggctc accgggcagg aggtctgcag ccggttcgcg cagagattca
      901 ggaccagcac cgccagcttg cgggccgcgc gcgcgttgca ggacggcggc gccggcgcca
      961 gaaccgcgga gcaggaatcg atctcgggaa ggccaagagc ggtgaacgtc gccgcggcgc
     1021 aggtggagac ggcttcgtcg agactttcgc cgcaggcctg cctccagaag ccgggtcccg
     1081 cggggtccgg caggcagggg gtcgtgtaga ggatctcgag cgccggccgc accagcggat
     1141 ccgggttctc gcgcgagtcg aaccgtcgcg cggtttgaag ccggtcctcg tcaccgatca
     1201 gcatccaacc gaagttggcc gtcggatcgt cgagccagga ctgcacgtcc tccaccatct
     1261 ccggggtcga gtcccaggca tacgggccgg tctgatcgac ggtcgcttcc ccgcgcgcga
     1321 ccgggtcgaa gtctcctccc ggctgcgtcc agaagacgtc atcgtggaag cggtggatcc
     1381 aggtcgcgtc gcccggctgg gatgggacgc cgttcccgcc gaacgaggac gacgccccct
     1441 cgccccagga ctgcgtcacg cggtgcaggc ggacggtcac cggaccggaa gcggccatcg
     1501 acaggttcag acgcagccgc accgcgtcga tggtggagcc agccggcact gcagacgcca
     1561 cgtcgaagcg gacgagcccc cggcgcagcg cttgcagcgt cgaggcgatc cggcccgcaa
     1621 agatggccgg gccggagccg gtcgccaggg acccttgcgg gtcctcgatg agcgtggcgt
     1681 cctgcgcggc agggacgacg gtggcgtcgg ccagcgcggc gcgccccatc gatacggcgg
     1741 ccagaagccc ccacaacgcc gtcctcctca gggatacccc ggcaccacga cccttttgcc
     1801 ccggcatgcg atccctccca ggacgcgaag attctgcgct tccacccgct tgtcaacgcg
     1861 aatctcttct ccgacgtcat gtcggccgcg cgcggtcgtc ttcgctccgg ggatcggaca
     1921 caatgcgatc attccacgag gggatgcggt gaccgagacg agctccggcg acgacgctct
     1981 ggcggggttc cggcgccggc atcccgaagc ggatcgctgg gccaccggat gcggcgtgat
     2041 ccggcacacc gacgagaccc aggcgcgcat cttctccatg gcggcggcgc tgggcgccgg
     2101 gcggccggcc ccggagggtt tcttcgcgcg gctcgaagcg gccgaccggg tcgtgagcgc
     2161 cgcgatgtgg ctcgtcgtgc acgagaccta cgcgag
//
LOCUS       DASRIQ010000371        12215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2479696, whole genome shotgun sequence.
ACCESSION   DASRIQ010000371 DASRIQ010000000
VERSION     DASRIQ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12215
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2479696"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFD06_08910"
     CDS             <1..547
                     /locus_tag="VFD06_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HZN03689.1"
                     /translation="REGVRQLLVSAIGCNIAWGLIDAVFYVMGGLFRRAHHQRLGRAI
                     QAAPDENAAAAVVAEALDPTLAEVSSPEERRHFYRNVVQVARRLEPRRPRITRDDLWG
                     ALASFWLVVASTIPAAIPFLVFRDQPRFALRVSNALLIALIFLVGFRWARYTGGSGWR
                     IGLALSLVGVALVAVAVALGG"
     gene            complement(581..1663)
                     /gene="cax"
                     /locus_tag="VFD06_08915"
     CDS             complement(581..1663)
                     /gene="cax"
                     /locus_tag="VFD06_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HZN03690.1"
                     /translation="MLWLLLFAPLALILEHTGAPAPAIFFCAAIAIVPIASLIVRATE
                     SIAHVTGPAIGGLLNATFGNLPEMIIALVALHAGMVEMVRASVIGALLANLLLGLGMS
                     FLLGGMRFHVQDYNPLAARAYASMMFLAVISMAIPSAFHRFFGAEAGIPQSAILDKSV
                     AVVLLLTYGLYLYHQLRTHKESFAEEEKKGSGAAHGEASHEAIWSPARAGATLLAASA
                     GAAWLSEVLVGAAEATGHALGMSPTFIGLILLASVGGAAEIGSAIAMGRRNKMDLSVG
                     IALGSCIQIALFVAPLLVFLGGVIGPEPFTLSFTRGEMGFLLISVLLGAMVASDGRSN
                     WFKGVQLLAVYAMLAAVCYLVPALAQ"
     gene            complement(1668..2669)
                     /locus_tag="VFD06_08920"
     CDS             complement(1668..2669)
                     /locus_tag="VFD06_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZN03691.1"
                     /translation="MRHRNEAAGGSARLSGRGAWRAAIPRPLFALASALLAATAGARA
                     ACAQSIEPRLFSNAPVGMNFILGGYAHSTGGVLLDPSVPLEDASVEVHSTFVGYSRSI
                     GIAGRSGQIQGIVSYAWLSGEALLTSTGEIRTRDVNGLGDPSVRFMLNLHGAPALPAA
                     EFGGYRQDLVVGVSLQITTPLGQYDPDRLVNLGTNRWTFKPEFGVSKALGHWILEGTG
                     AVNLYTDNDDFFGGQHRSQDPIYSAQGHVIYNFRPGLWAAFDATWYTGGRTTIDGVEG
                     DDLQRNWRAGLTGAVPFDRRNSLRVYLSNGVTTRSGDDFLLAGAAWQHRWGAGVTRR"
     gene            2736..3425
                     /locus_tag="VFD06_08925"
     CDS             2736..3425
                     /locus_tag="VFD06_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03692.1"
                     /translation="MRRPIAITGARRLLRGALLAAAFLSRAVLAADTPDPAGPAASSP
                     GPAMPGAWDYSLSGNGYLFPGDGDFLLGIATADRGPLHLEARYNYEDRDTASFWGGWN
                     WSTGETVEFALTPMLGVAVGDTDAILPGLKLSLAWKQIDYYLESEIAFNLHDHDEDFV
                     YGWSEIGWTPVDWLRVGFVGQRTRVYDTGLDIQRGLLAQGIVGRFVFGLDWFNPASDD
                     EFVVALLGVEF"
     gene            3479..4306
                     /locus_tag="VFD06_08930"
     CDS             3479..4306
                     /locus_tag="VFD06_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HZN03693.1"
                     /translation="MAPRTSLFAAALAFHALLALAPVLLVLIPTATRLFGEAETQEAL
                     ADVIDRFAGRGAAQVGLALLALVASAPAPRAGTLFGIALLLYFGSTFFAQFRAALDAI
                     WEAGPQGLRGALVARAVSLVEAIVAFAAALMILAAGATRSIARSALDYFGAAAPLGVS
                     GWTRLGTLLMTLLALAMIFRYIPSVRPRPGWGAVLAGTVPATFLLNVATDLFGLLVTK
                     SALASLYGTAASLVMFLLWVYYSAWVVLFGAEVCRAWGETTANAGPTSRRETITART"
     gene            complement(4418..5845)
                     /locus_tag="VFD06_08935"
     CDS             complement(4418..5845)
                     /locus_tag="VFD06_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HZN03694.1"
                     /translation="MADEGAARRASRRTAAVRVAAFAVFAALWFAPAPEGLTPAAWHL
                     FAIFAATILAVVAGALPILTAAVLAVAAAVLTGTLSPADAYAGFGNPTILLIIVAFLV
                     ARAVVKCGLGQRLGYRAISLFGRSTLGLSYSIFAVDALIAPAFPSSTARSGVLYPLVF
                     SVAEAAGAKPDREDRRRLGRFLMFSGVASLSLSSALWLTAHAANPLGAEIAKQNGVDI
                     GFGSWLLAASVPTLAAMALMPLVLYKVIRPEVTATPEAPAEARRALAALGPITRDQKI
                     VAATFIGMVALWGASATFGIDTTAVAFLGLGVLLATSVLTLGDIAKEGDVLATFIWFA
                     ALLTLSDQLNRLGFMEFLGRRLAMRLDGLSPLPAGILLVVVYVLLHYLFVSQTAHLLA
                     LFGVFLGVGVTLGVPAAPLAFLLLFATDFFASITPQASSANLLFAGSGYLSQGDLYRL
                     GAIATGFSLLVYLVVGTPWLLWVAR"
     gene            complement(5864..7150)
                     /locus_tag="VFD06_08940"
     CDS             complement(5864..7150)
                     /locus_tag="VFD06_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZN03695.1"
                     /translation="MSRPVHHPRVWGAIALLIAIAPIPARGEETAQAPAATIETTVEA
                     GEAGAEEPRRRVTKNEFDWPVTTFHYGLGFLLEYDSYSQDDESKQQLSLEPDAGVRDA
                     RLLFRGRFKTERPLTWSIGYMYDHSNRSWHFRQTGLMIGFPELKGNLFIGRTKEGYSM
                     TKVMTGYHIPTMERSPALDAFVPILADGIKWLGYFPGPEIFFNLGVYTDSLEESASFA
                     TYDHQQVLRVGWLPIQDEKAGKLLHVAVMGRNGEPDEGFLQQRARPEANFAPYFVDTG
                     RFAANHVLTTGIETYYRDGPWLFGAEYNFQDIDTTDDRHPRFQGGNIIAAWIVTGETR
                     PYNVAGGGYFTDFSPKRPVFAGGPGAWEVVLNMSYLDLDDGTFQGGKVWRVTPMVNWH
                     LVDYLKLQMAYGYSVLDRFGLEGSTQFFQARLVTYF"
     gene            complement(7164..8228)
                     /locus_tag="VFD06_08945"
     CDS             complement(7164..8228)
                     /locus_tag="VFD06_08945"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006229544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HZN03696.1"
                     /translation="MKTPLRILGMLGATLVLASSTFAQAPKKKIVILATGGTIAGAAL
                     SPTDPGYQSGAVGVDILIKAVPDLKKIADVTGEQIASIGSQDMNDEVWLKLAKRANEL
                     LANAGVDGIAITHGTDTLEETSYFLQLVIKSDKPVVMTGSMRPSTAMSADGPLNIYNA
                     VAVAADPKARGRGVLVVVDDDIHSAHDIVKTHTTDVATFSSKEAGLVGVALFGANIWY
                     RTPASVHTKASELGLGDVKSLPRVDIIYAHANMSPDLITAAAQNGAKGIVIAGVGDGN
                     MTAPALEAVKKVIKQGVVVVRSSRTNAGIIRRNIEVNDDQIGTVASMELNPGKARALL
                     QLALLKSQDAKKIQDYFDRY"
     gene            complement(8255..9823)
                     /locus_tag="VFD06_08950"
     CDS             complement(8255..9823)
                     /locus_tag="VFD06_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HZN03697.1"
                     /translation="MTRKRMPRPVTLMGAALVAAACLSNDPLFAQAKKTTPPPAKTAP
                     TPAPAKTRTEKDLLGEKQVPAEAYYGVQTMRGMENFQLSGVAINHYPGYVEAWAIVKL
                     AAARANTEVGAMKPERLAMIEKAAKAVLDGKYHDQFMVDWYQGGAGTSTNMNANEVLA
                     NIGLELGGHKKGEYQFLEPHDDLNMSQSTNDSYPTAIKVALMLRNDRLVAELQQLVRS
                     FRAKGNEFLTVVKMGRTELQDAVPMTVGQEFHAFAAALEGEIALLRDAEKYLYAENMG
                     ATAIGTGINVPKGYPEKCAAHLAKITGKPIVMADDMIAATWDQQGFVVYSSALKSVAI
                     KLAKIASDLILLSSGPRAGLFEINLPALQPGSSIMPGKVNPVVPEVVGIVAFRVMAND
                     YAVSLAAHSGQLQLNAYEPLNGLAPIESQHLLFNVSKLFRSKCIDGITVNKKQLEHYM
                     ETTVGIVTALNPVIGYEKATELAAEAYKSGKGILEVIREKKVLTEAQIADLLDAIKLT
                     NLDPKQYEKYKDIK"
     gene            complement(9971..10162)
                     /locus_tag="VFD06_08955"
     CDS             complement(9971..10162)
                     /locus_tag="VFD06_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03698.1"
                     /translation="MRIRRQPRLAAFALLAAVAVVAVTTPALAQVPAKPQVDAAMKAI
                     ADVSNALGGNPYASKPKET"
     gene            complement(10159..11394)
                     /locus_tag="VFD06_08960"
     CDS             complement(10159..11394)
                     /locus_tag="VFD06_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HZN03699.1"
                     /translation="MKSIFGHSLVVTGFVVAALAIAPSASAQETDKTRMDIYGFAMLD
                     MGYQTKQNDPDWFDVLRPTKLPVFEDQFGGDEHFFAGVRQSRFGVKTFTPTSKGEIKT
                     IFEFELFGVGPDAGQTTFRLRHAWGELGHWGAGQSWSPFMDPDVFPNSIEYWGPSGMV
                     FFRNVQVRWMPLQGENEVYVALERPGASGDPGVLVGSPGVDLTGRYPLPDLSGHYKRS
                     GDWGHIQIAGIVRKMEWDDTDPAAPDASGSATGWGVNLSSNLKFSKDILKLQVVYGEG
                     IANYMNDATADVGAVTGPDVGEALPLVGVVAFYDRTWSEKWTSTFGYSMIDIDNSNAQ
                     APDAFSKGQYALANLLYYPVKNLMWGGEIQWGDRTNNDDDGTTLDNFGNAVPVDSFDD
                     LRIQFSVRYNFSKTLGGAS"
     gene            complement(11420..11548)
                     /locus_tag="VFD06_08965"
     CDS             complement(11420..11548)
                     /locus_tag="VFD06_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03700.1"
                     /translation="MYAPLLARLKPETRRLLLRGNYERLFDAARTRVRAWEKEHVK"
     gene            complement(11845..>12215)
                     /locus_tag="VFD06_08970"
     CDS             complement(11845..>12215)
                     /locus_tag="VFD06_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate-lyase 1-activating enzyme"
                     /protein_id="HZN03701.1"
                     /translation="FDLVLLDLKMWDDARHRKLTGQPVGPVHDFARRLAARRRPVWVR
                     FVLVPGLTDDAENVEAIARFAADLGTVERVDVLPFHQMGRFKWKSLKLDYTLESVEPP
                     SHALVDDVVGRFKAAGLKAY"
BASE COUNT         1891 a         4156 c         4149 g         2019 t
ORIGIN      
        1 gcgcgaaggg gtgcgccagc tcctggtctc cgccatcggc tgcaacatcg cctggggcct
       61 catcgacgcc gtcttctacg tcatgggagg gctgttccgg cgcgcgcacc accaacgcct
      121 gggacgggcg atccaggccg cgccggacga gaacgccgcg gccgcggtcg tcgccgaggc
      181 gctcgacccc acgctcgcgg aggtctcgtc accggaggag cggcggcact tctaccggaa
      241 cgtggtccag gtcgcacgac gactcgagcc gcgcaggccc cgtatcaccc gcgacgatct
      301 ctggggcgcg ctcgcgagct tctggctcgt cgtcgcctcg acgattccgg cggcgatccc
      361 gttcctcgtc ttccgggatc agccgcgctt cgcgctgcgg gtgtcgaatg cgctcctcat
      421 cgccctgatc ttcctcgtcg gcttccgctg ggcgcgctac accggcgggt ccggatggcg
      481 catcggcctg gcgctgtcgc tggtcggggt cgcgctcgtc gccgtcgccg tggcgctcgg
      541 cggctgatcc ggccgcgcgc ggcggcgcac cagactcgtg tcactgcgcc aacgccggca
      601 ccaggtagca gacggcggcg agcatggcgt acaccgccag gagctgcacc cccttgaacc
      661 agttggatcg tccgtcggag gcgaccatcg cgccgagcag caccgagatg agcaggaatc
      721 ccatctcccc gcgggtgaac gacagagtga atggttccgg accgatgacg ccgcccagga
      781 agaccaggag cggcgcgacg aacagcgcga tctggatgca ggagccgagc gcgatgccga
      841 ccgagaggtc catcttgttg cggcgcccca tggcgatggc ggagccgatc tccgccgccc
      901 ctccgacgga ggccagcagg atcaggccga tgaaggtcgg cgacatgccg agcgcgtgcc
      961 cggtcgcctc ggcggcaccc accagcacct cgctcagcca cgccgccccc gcggaggcgg
     1021 ccagcagcgt ggcgccggcc cgcgccgggc tccagatcgc ctcgtgcgag gcttccccgt
     1081 gcgccgctcc gctccccttc ttctcctcct cggcgaacga ctccttgtgg gtccgcagct
     1141 ggtggtagag atagagcccg taggtcagga ggagcaccac cgccacgctc ttgtccagaa
     1201 tcgcggactg cgggatcccc gcctcggcgc cgaagaagcg atggaaggcg ctcgggatcg
     1261 ccatgctgat caccgcgagg aacatcatcg acgcataggc gcgcgccgcc agcgggttgt
     1321 agtcctgcac gtggaagcgc atcccgccga gcaggaacga catccccaga ccgagcagca
     1381 ggttcgcgag gagcgctccg atgaccgaag cgcgcaccat ctccaccatg ccggcgtgca
     1441 gcgccacgag ggcgatgatc atctccggca ggttgccgaa cgtggcgttc agcaggccgc
     1501 cgatggccgg cccggtgacg tgggcgatcg attccgtcgc gcggacgatg agcgaggcga
     1561 tcgggacgat ggcgatcgcg gcgcagaaga agatcgccgg cgccggcgct ccggtatgct
     1621 cgaggatcag cgccagggga gcgaacagga gcagccagag catcgggtca cctcctggtg
     1681 acgccggcgc cccaacggtg ctgccaggcg gcgccggcga gtaggaaatc gtcgccggaa
     1741 cgggtcgtca caccgttgct cagatagacc ctcagggagt tgcgccgatc gaaaggcacc
     1801 gccccggtga gcccggcgcg ccagttgcgc tgcaggtcgt ctccctccac gccgtcgatc
     1861 gtggtccggc ctcccgtgta ccaggtcgca tcgaacgccg cccacaggcc ggggcggaag
     1921 ttgtagatga cgtgcccctg ggcggagtag atggggtcct gcgaacggtg ctgcccgccg
     1981 aagaagtcgt cattgtcggt gtagagattg acggcgccgg ttccctcgag gatccagtgt
     2041 ccgagcgcct tcgagacgcc gaactccggc ttgaaggtcc agcggttggt gccgaggttg
     2101 accaggcggt cgggatcgta ctggccgagc ggcgtcgtga tctgcaggct gactccgacc
     2161 acgaggtcct ggcgatagcc gccgaactcc gccgccggca gggccggggc gccgtggaga
     2221 ttgagcatga agcgcaccga cgggtcgccg aggccgttga cgtcgcgcgt gcggatctcg
     2281 ccggtcgagg tcagcagcgc ctctcccgag agccaggcgt aggagacgat cccctggatc
     2341 tgccccgatc ggcccgcgat gccgatcgat cgagagtaac cgacgaaggt cgagtgcacc
     2401 tcgacgctcg catcctcgag cggcaccgac ggatcgagca gcacgccgcc ggtggagtgg
     2461 gcgtacccgc cgaggatgaa gttcatgccg accggcgcgt tggagaagag tcgcggttcg
     2521 atcgactggg cgcacgccgc gcgggcgccg gcggtcgccg ccaggagcgc cgaggcgagc
     2581 gcaaacaagg ggcgcgggat cgcggcgcgc caggcgccgc gccccgacag gcgggcggag
     2641 ccccccgctg cttcgttccg atgcctcacc cggcggcccc cccgctgtgg ggcgggaatg
     2701 tagcacgcgg cgccccgtga taccctcccg ccgctttgag gcgcccgatc gcaatcaccg
     2761 gggcacgacg cctgctgcgc ggcgctctcc tcgcggcggc gttcctgtcg cgggccgtcc
     2821 tggcggccga cacaccggat ccggccggac ctgcggcctc ctcgcccggc ccggcgatgc
     2881 ccggcgcctg ggactactcc ctctccggca atgggtacct gttcccggga gacggggact
     2941 ttctcctcgg gatcgccacc gccgatcgcg gtccgctgca cctcgaggcc cgctacaact
     3001 acgaagaccg ggacaccgcc tcgttctggg ggggctggaa ctggtccacc ggcgagacgg
     3061 tggagttcgc cctcacgccg atgctcgggg tcgccgtcgg ggacaccgac gcgatccttc
     3121 ccggcctcaa gctgagcctc gcctggaagc agatcgatta ctacctggaa agcgagatcg
     3181 cgttcaacct tcacgaccac gacgaggact tcgtctacgg gtggagcgag atcggctgga
     3241 cgcccgtcga ctggctgcgg gtggggttcg tcgggcagcg gacgcgcgtc tacgacaccg
     3301 gtctcgacat ccagcgcggc ctcctggcgc aggggatcgt cggccgcttc gtgttcggcc
     3361 tcgactggtt caatccggcg agcgacgacg agttcgtcgt ggcgctcctc ggggtcgagt
     3421 tctgagcgag ctgctgcgac gctaccggcg gatcctggcg atcgcctcgc gtcgctggat
     3481 ggccccacgc acttcgctgt tcgccgccgc actggcgttc cacgccctgc tcgccctggc
     3541 gccggtcctc ctggtgctga tccccaccgc cacgcgcctc ttcggagaag cggagaccca
     3601 ggaggctctc gcggatgtga tcgatcgttt cgccgggcgc ggcgcggccc aggtcgggct
     3661 ggcgcttctc gcgctcgtgg cctcagcacc ggccccgcgg gccgggacgc tgttcggcat
     3721 cgccctgctg ctgtacttcg gctcgacctt cttcgcgcag ttccgcgccg cgctcgacgc
     3781 gatctgggag gccggcccgc aggggcttcg gggcgcgctc gtcgcgcggg ccgtgtccct
     3841 cgtcgaggcg atcgtcgcct tcgccgccgc cctgatgatc ctggcggcgg gagcgacccg
     3901 ctcgatcgcg cgatcggcgc tggactactt cggcgccgcc gccccgctgg gggtctcggg
     3961 atggacgcga ctcgggacgc tcctgatgac actgctcgcc ctcgcgatga tcttccgcta
     4021 catcccctcg gtgcgcccgc ggcccggctg gggcgcggtg ctggcgggca cggttccggc
     4081 aaccttcctg ctcaacgtgg cgaccgacct gttcggcctc ctcgtcacca aaagcgccct
     4141 cgcctcgctc tatgggacgg ccgcgagcct ggtgatgttc ctcctgtggg tgtactacag
     4201 cgcctgggtc gtgctcttcg gcgccgaggt gtgccgcgcc tggggcgaga cgaccgcgaa
     4261 cgccggcccc acgtcgcgcc gggaaacgat cacggctcgt acatgaacgc ggcgatgccg
     4321 cgccggatgt tgcgcggtcg tcggggagcg gttgagccgc ttgtcgcgca ggatctcgaa
     4381 ccccttcttc acatcgttcc gtctcggttg aacccgttca ccgggccacc cacagcagcc
     4441 acggcgtccc gaccaccagg tacaccagca gcgagaagcc ggtcgcgatc gctccgaggc
     4501 ggtacaggtc cccctgcgac aggtagccgc tgccggcgaa caggaggttg gcggaggacg
     4561 cctgcggcgt gatcgaggcg aagaagtcgg tcgcgaagag cagcaggaac gccagcggcg
     4621 ccgccggcac gcccagcgtg acgccgacgc cgaggaacac cccgaacagc gccagcaggt
     4681 gcgccgtctg gctcacgaac aggtagtgca ggaggacgta gaccacgacg agcaggatcc
     4741 ccgccggcag cggcgacagc ccgtcgagac gcatcgccag gcgtcgtccg aggaattcca
     4801 tgaagccgag ccggttcagc tgatcgctca gcgtcagcag cgccgcgaac cagatgaacg
     4861 tggcgaggac atccccctcc ttggcgatgt ccccgagcgt caggaccgac gtggccaaga
     4921 gcaccccgag cccgaggaag gcgacggcgg tcgtgtcgat gccgaacgtc gccgacgccc
     4981 cccacagcgc caccatcccg atgaaggtgg cggcgacgat cttctgatcg cgggtgatcg
     5041 gtccgagagc ggcgagcgcc cggcgcgcct cggccggggc ctcgggcgtc gccgtgactt
     5101 cgggccggat gaccttgtag aggacgagcg gcatgagcgc catcgccgcg agcgtgggaa
     5161 ccgaggcggc gagcaaccac gatccgaacc cgatgtcgac accgttctgc ttcgcgatct
     5221 cggcgccgag cgggttcgcc gcgtgcgccg tgagccacag ggccgaggag aggctcaggc
     5281 tggcgacgcc agagaacatc aggaagcgcc ccaggcgccg gcgatcctcc cggtccggct
     5341 tcgcccccgc cgcctcggcg accgagaaga ccagcgggta cagcaccccc gatcgggccg
     5401 tgctgctcgg gaaggccggg gcgatgaggg cgtccaccgc gaagatgctg tacgacagcc
     5461 cgagcgtcga tcgcccgaaa agggagatgg cgcgataccc cagccgctgc ccgaggccgc
     5521 acttcacgac cgcgcgggcc acgagaaagg cgacgatgat cagcaggatg gtcgggttgc
     5581 cgaaccccgc gtaggcgtcc gccggagaga gcgtccccgt gaggaccgcc gcggcgacgg
     5641 cgaggacggc ggcggtcagg atcggcaggg cgccggcgac gaccgcgagg atcgtcgccg
     5701 cgaagatcgc gaacaggtgc caggcggccg gggtcagccc ttcgggagcg ggggcgaacc
     5761 agagggcggc gaagacggcg aacgcggcca cccgcaccgc cgccgtgcgc cgggaagcac
     5821 gccgtgccgc cccctcgtcc gccatggcgt cagcgttctc cgactagaag taggtcacca
     5881 gccgcgcctg gaagaactgc gtgctccctt cgagcccgaa ccggtccaac acggaatacc
     5941 cgtaggccat ctgcaacttc aggtagtcga cgaggtgcca gttgaccatc ggcgtcaccc
     6001 tccagacctt gcccccctgg aaagtcccgt cgtcgaggtc gaggtacgac atgttcagga
     6061 cgacctccca ggcgccgggc ccccccgcga acaccgggcg cttcgggctg aagtcggtga
     6121 agtacccgcc gcccgccacg ttgtagggac gcgtctctcc ggtcacgatc cacgccgcga
     6181 tgatgttccc cccctggaag cgcgggtgcc ggtcgtcggt cgtgtcgatg tcctggaagt
     6241 tgtattccgc accgaacagc cagggaccgt cccgatagta cgtttcgatg ccggtcgtga
     6301 ggacgtggtt ggcggcaaac cttccggtgt ccacgaagta cggggcgaag ttcgcctcgg
     6361 gacgcgcgcg ctgctgcagg aaaccctcgt ccggctctcc gttgcggccc atcacagcga
     6421 cgtggagaag cttgccggcc ttctcgtcct ggatcggcag ccagccgacc cgcaggacct
     6481 gctggtggtc gtaggtggcg aaggacgcgc tctcctcgag actgtcggtg tacacgccga
     6541 gattgaaaaa gatctcgggc cctggaaaat agcccaacca cttgatcccg tccgcgagaa
     6601 tcggcacgaa cgcgtcgagg gccggagagc gctccatggt cgggatgtgg taccccgtca
     6661 tgaccttggt catcgagtag ccttccttcg tccggccgat gaagaggttt cccttgagtt
     6721 cgggaaatcc gatcatgaga ccggtctggc ggaagtgcca cgacctgttc gaatggtcgt
     6781 acatgtaacc gatcgaccat gtgagcggcc gctcggtctt gaagcgcccc cggaacagga
     6841 ggcgggcgtc ccgcactccc gcgtcgggct ccagggagag ttgctgcttg ctctcgtcat
     6901 cctgggaata ggagtcgtat tcgagcagga accccaggcc gtaatggaag gtcgtgaccg
     6961 gccagtcgaa ttcattcttg gtgacgcgcc ggcgcggctc ctcggcacca gcctctcccg
     7021 cctcgacggt cgtttcgatc gtcgcggcgg gggcctgtgc cgtctcctcg ccgcgcgccg
     7081 ggatgggggc gatcgcgatg aggagggcga tcgctcccca cacccggggg tgatgaacgg
     7141 gtcgagacac agcgcgcgcg acgtcagtag cggtcgaagt aatcctggat cttcttcgcg
     7201 tcctgcgact tcagcagcgc cagctgcagc agcgcgcgcg ccttgcccgg gttgagctcc
     7261 atggaggcca ccgtgccgat ctgatcgtcg ttgacctcga tgttgcgccg gatgatgccg
     7321 gcgttggtgc gggacgatcg cacgacgacc acgccctgct tgatcacctt cttgaccgct
     7381 tccagggccg gcgccgtcat gttgccgtcc cccaccccgg cgatcacgat ccccttcgcg
     7441 ccgttctggg ccgcggcggt gatcaggtcg ggggacatgt tcgcgtgggc gtagatgatg
     7501 tccacgcgcg gcagggactt cacgtcgccg aggcccagct cgctggcctt ggtgtggacc
     7561 gaggccggcg tccggtacca gatgttggcg ccgaagagcg ccacgccgac gagcccggcc
     7621 tccttggagg agaaagtggc cacgtccgta gtgtgggtct tgacgatgtc gtgggccgaa
     7681 tggatgtcgt cgtcgacgac cacgaggacg ccgcgaccgc gcgccttcgg atcggccgca
     7741 accgcgacgg cgttgtagat gttcagcggc ccgtcggcgc tcatggcggt cgaggggcgc
     7801 atcgagccgg tcatcaccac cggcttgtcg ctcttgatga cgagctgaag gaaatacgac
     7861 gtctcctcca gcgtgtcggt gccgtgcgta atcgcgatcc catcgacgcc ggcgttcgca
     7921 aggagctcgt tggcgcgctt cgcgagcttc agccagacct catcgttcat gtcctggctg
     7981 ccgatgctgg cgatctgttc gccggtgacg tcggcgatct tcttgagatc ggggaccgcc
     8041 ttgatcagga tgtccacccc cacggcgccg gactggtacc cgggatccgt cggcgacagg
     8101 gcggcgccgg cgatcgtgcc gccggtcgcc aggatcacga tcttcttctt gggggcttgg
     8161 gcgaaggtgc tggaggcgag gaccagcgtc gctccgagca ttccgaggat ccgcagtggg
     8221 gtcttcatgg tgttgcctcc cgttgattgc gttttcactt gatgtccttg tacttctcgt
     8281 actgcttcgg atccaggttg gtgagcttga tcgcgtccag cagatcggcg atctgcgcct
     8341 cggtcagcac cttcttctcg cggatgacct cgaggatccc cttgccgctc ttgtacgcct
     8401 cggccgccag ctccgtcgcc ttctcgtagc cgatcacggg attgagcgcg gtgacgatcc
     8461 cgacggtggt ctccatgtag tgctccagct gtttcttgtt cacggtgatc ccgtcgatgc
     8521 acttggagcg gaacagcttc gagacgttga acagcagatg ctgcgactcg atcggcgcga
     8581 ggccgttcag cggctcgtac gcgttgagct gcagctggcc gctgtgagca gcgagcgaga
     8641 cggcgtaatc gttcgccatc acgcggaagg cgacgattcc caccacctcg ggcacgaccg
     8701 ggttgacctt ccccggcatg atcgacgacc cgggctgcag cgccggcagg ttgatctcga
     8761 acagtcccgc ccgtggcccc gagctgagca ggatcaggtc gctcgcgatc ttggccagct
     8821 tgatcgcgac gctcttgagc gcgctcgaat agaccacgaa cccctgctgg tcccaggtcg
     8881 cggcgatcat gtcgtcggcc atgacgatcg gcttgccggt gatcttcgcg aggtgcgccg
     8941 cgcatttctc cgggtagccc ttcggcacgt tgatcccggt gccgatcgcg gtggccccca
     9001 tattctcggc gtagaggtac ttctcggcgt cgcgcagaag ggcgatctcc ccctccagcg
     9061 cggcggcgaa ggcgtggaac tcctggccga ccgtcatcgg gactgcatcc tgcagctcgg
     9121 tccgccccat cttcacgacg gtgaggaact cgttcccctt cgcccggaag gagcgcacca
     9181 gctgctgcag ctcggcgacg agccggtcgt tgcgcagcat cagggcgacc ttgatcgcgg
     9241 tcgggtagga atcgttggtc gactgcgaca tgttcaggtc gtcgtgcggc tcgaggaact
     9301 ggtactcccc cttcttgtgg cccccgagct cgagcccgat gttggcgagc acctcgttgg
     9361 cgttcatgtt cgtcgaggtc ccggcgcccc cctggtacca gtccaccatg aactggtcgt
     9421 ggtatttgcc gtcgaggacc gccttggccg ccttctcgat catggccaga cgctccggct
     9481 tcatcgcgcc cacctcggtg ttggcgcgcg cggccgccag cttgacgatg gcccacgcct
     9541 cgacgtagcc gggatagtga ttgatggcga cgccggagag ctggaagttc tccatgcccc
     9601 tcatggtctg gacgccgtag tacgcctccg ccgggacctg tttctcgccg agcaggtcct
     9661 tttcggtcct cgttttcgcg ggcgccgggg tcggagcggt cttcgccggc ggcggggtgg
     9721 tttttttcgc ctgagcgaac aggggatcgt tcgacaggca ggcggccgcc acgagcgcgg
     9781 ctcccatgag cgtcacaggt cgaggcattc gtttccgggt catcgcatca cactccttgc
     9841 gtcacttctc acgtcttttc tgaatctcga gcagcatgct cacgtctcgt gccccgggcc
     9901 ggcgacggcc ggatggccgc cgccggcacc aggcaggcag cccgggcccg gatcgcatcc
     9961 ggcccgggct tcacgtctcc ttcggtttcg acgcgtacgg gttcccgccg agcgcgttcg
    10021 agacgtcggc gatggccttc atggcggcat cgacctgggg cttcgccggg acctgcgcca
    10081 gcgccggcgt cgtgaccgcg acgacggcca cggcggccag gagggcgaac gccgcgaggc
    10141 gcggttgcct acgaatcctc acgaggcgcc accgagggtt ttcgagaagt tgtagcggac
    10201 ggagaactga atccgcaggt cgtcgaagct atccaccggc accgcattcc caaagttgtc
    10261 aagggttgta ccgtcgtcgt cgttgttcgt gcgatcgccc cactggatct ccccacccca
    10321 catcaggttc ttgaccgggt agtagaggag gttcgccagg gcgtactgcc ccttgctgaa
    10381 agcgtccgga gcctgggcgt tgctgttgtc gatgtcgatc atggagtatc cgaatgtcga
    10441 cgtccacttc tcgctccagg tgcggtcgta gaaggcgacg acgcccacaa ggggcagagc
    10501 ctctccgacg tcaggacccg tgaccgcgcc aacgtcggca gtcgcgtcgt tcatgtagtt
    10561 ggcgatcccc tcgccataga cgacctggag cttgaggatg tccttggaga acttgaggtt
    10621 cgaggagaga ttgacgcccc agccggtggc gcttcccgag gcgtcgggag cggctggatc
    10681 cgtgtcgtcc cactccatct tccggacgat cccggcgatc tggatgtggc cccagtcacc
    10741 gctcctcttg tagtgccccg acaggtccgg gagcgggtag cgccccgtga ggtcgacccc
    10801 cggcgagccg acgaggaccc ctggatcgcc gctggctcca ggccgctcga gagcaacata
    10861 cacttcgttc tccccctgca gcggcatcca gcgcacctgg acgttgcgga agaagaccat
    10921 gccgctcggc ccccagtact cgatggagtt cgggaagaca tcgggatcca tgaacgggct
    10981 ccaggactgg ccggcgcccc aatgcccgag ctcgccccag gcgtgacgca ggcggaacgt
    11041 ggtctggccg gcgtcggggc cgacgccgaa cagctcgaat tcgaagatcg tcttgatctc
    11101 ccctttcgac gtcggcgtga aagtcttgac cccaaagcgg ctctggcgca cgccggcgaa
    11161 gaagtgctcg tcgccgccga actggtcctc gaagacgggg agcttggtcg gtcgcagcac
    11221 gtcgaaccag tcggggtcgt tctgcttggt ctggtagccc atgtcgagca tggcgaagcc
    11281 gtagatgtcc atccgcgtct tgtccgtctc ctgcgcggat gcggatggtg cgatggcgag
    11341 cgccgccacg acgaatcccg tcaccacgag gctgtggccg aagatactct tcatgaaggc
    11401 cctcctatgc gttgggtggt cacttcacgt gctccttttc ccaggcccgc acccgtgtgc
    11461 gggccgcgtc gaatagccgt tcgtagttcc cccggagcag caaccgtcgc gtctccggct
    11521 tcagccgcgc caggagcggc gcgtacatgg gaaccatacg caaaactacg taggcctcga
    11581 aggaggcagc acggaaccgg gcaaagcgaa gcgtcactga attccggccc tccggcgggt
    11641 cgttccagac gaatgccggc gaagcatagg cgaacaggct tgttgaccgc tacccctccc
    11701 caggtcggcg cctcggagcc ctgctgcgtc agacccccag aagccgcgcg gcgtgcgggg
    11761 cgatcatggc ttcctcgttc gtggcgatca cccatgcgga gacctgtctg accagggtcg
    11821 agatccgcct tcgagcccgg gcgttcagta cgccttcagg ccggccgcct tgaaccgtcc
    11881 gacgacgtcg tcgaccagcg cgtgggaggg gggctcgacc gactcgagcg tgtaatcgag
    11941 cttgagcgac ttccacttga agcggcccat ctggtggaag ggaaggacat ccacccgctc
    12001 gacggtcccg aggtcggcgg cgaagcgggc gatcgcctcg acgttctccg cgtcgtcggt
    12061 cagcccgggg acgagaacga agcgcaccca gaccggccgg cgcctggcgg ccaggcgccg
    12121 cgcgaagtca tgcacgggac cgaccggctg cccggtgagc ttccggtggc gcgcgtcgtc
    12181 ccacatcttg aggtcgagga ggaccagatc gaacg
//
LOCUS       DASRIQ010000372         7388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2224934, whole genome shotgun sequence.
ACCESSION   DASRIQ010000372 DASRIQ010000000
VERSION     DASRIQ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224934"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="VFD06_08975"
     CDS             complement(<1..1862)
                     /locus_tag="VFD06_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR05002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat
                     NxxGxxAF-containing protein"
                     /protein_id="HZN03702.1"
                     /translation="MNTHLKKTCSSPIRLAVVLGLMFCALAPAHAQSLMTTNGDFLAW
                     PDTDVPGLPGVYFGGSIQLPTVAEDGTLFFQSDMYGAGVSGTNYKGLFSGTSAADLSM
                     VARWSGPAPGLPGLALINSAGTQGIGSTALLSPSGGYKVWSSFLSGAGVNTNNDTALF
                     RSSPGGSVLVAREGDVAPGTVGALFSGNLKSEYQFTQVNRTGRVLFQSSLIGGDATGG
                     NNGAIFTGTPGALSILRRLGDAVLPGPVRAAGMGTIQQMDSNGRVLYDVTLSGAGVTA
                     ANDSSLWFYTPGAGSTLLVREGDPAPGTSGATFGNLQDNWYPGCSPTSLTRSGKYEMA
                     VDLKGGDVTYGVNDRALYVGAVGGGLTMIARAGDPAPGTDAFFQGFSPFYSLINDAGD
                     VAFQATLNGGTSDPTNNSGIWTWKSGTLSLVVRSGAPVPGFPPIPDVPVPQTTYGTFI
                     GWAMSFNDLGQIFIQGNLRGGEIMGGVDDRELLVFDPIKGLIVVARSGEEIEGVPGSF
                     RTFRVGGYVQFSNTDGVSHALGNTGRVAVWALFTEGGAIATVDLNCYPATTYGIDGDG
                     DGRGDASTRVSVCSGGTPPNGYIPNATDCNDGDPAVYTPYYQDADGDGRGDKWV"
     gene            complement(1878..4568)
                     /locus_tag="VFD06_08980"
     CDS             complement(1878..4568)
                     /locus_tag="VFD06_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03703.1"
                     /translation="MRRNDRSLLAGCLLAALLIAGVATAAFAGGPLYTYDYENRIPYA
                     WNMATWPNGEVPVYTDLGSLAVLSNARASEEVLFAADQWSLVPTSSFRALIAGDFSAL
                     DLDDIVDVNVESVLGAWNGGGIHVVYDSDGSIMTNFFGLPPEGVLGIASIDYVADDGP
                     EILEAWMVLSGPGIYPNDPDGVGFQGVITHEMGHALNLGHSQANGAVWFPAVYDLPQP
                     EGCNAPWAGVSDATQVETMYPFSTPEPGNAGEYMGTVDRLDDMSALSDLYPAPGYPAS
                     RGTIRGQILDASGAPVTGVNVIARNVLDPFNDFSSYISGQVSKGQAGPDGSFVLNDLT
                     PGARYVIYTDNLASGAFSVPRVIVLPGPEEYFNGATESGDSAHDDRCAWTTVPAQPGA
                     PATANITFNRYPGAPIFLTAPGNSEPTDITPDGSIVVGGLSNTDGPIFRWDVDAGTFE
                     DIGGFRVGTPSISDDGTKIASTALDTDGSVKAAIHQNNAWTLLPPVPGSTPCTQDGPT
                     TYNSAWDISGDGSTVVGLSYGDACFRGGIRAFKWTAAGGSVALPKFSSFNNMSRANGV
                     NYDGSVIVGLDETTSGFWRGAYWQNGVVKLITRNGQSVLSAVDVSRDGQYVVGQSSLP
                     ATSNSAWRYPVGAMNVELIGLFTGYNNSVTNAISDDHRVITGYTSSNTTGAFTPTIWT
                     AGLHWSSFNTFLLAQGVNLTDAVMLGATAMSADGHVLTGTLASRFGNVGFVVKTPTSV
                     VCHAPAGNPTQLQTTTVSFPQGLDAALAAGDTLGPCQCNATAPTGIAALTVGQPAKGI
                     AQVVWSPVAAATGYDLARGSLSALRSSHGDFSAATTDCLESDLTATSRDDADAPGVGD
                     GFWYLVRAVSCGGSATFDSGAPSQVGSRDAEMQASPSTCP"
     gene            complement(4565..5350)
                     /locus_tag="VFD06_08985"
     CDS             complement(4565..5350)
                     /locus_tag="VFD06_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03704.1"
                     /translation="MTGGRAGVRALPVLLALAWAAPGGTALAMSLTSANVVDLLGEAN
                     AIVVGRVEAVTDGIDERGIPYTEITLDISETIRGDLAETYTFRQFGLLNPRRTADGRR
                     KMMPGPAGFPKYVPGENVLLFLRPAAAWTGFRMPAGATHGKFTIDAGRVENGMGNAGL
                     FRDVHLDKGLVTDRDKRLLTTAAGPLNPDAFLSFVRRAVEERWIENGRMTHAKGRGGP
                     RTPPRSDPGDAQRPTPAGKPDAPNAPTAPLDPNASIALPKGGR"
     gene            5620..6075
                     /locus_tag="VFD06_08990"
     CDS             5620..6075
                     /locus_tag="VFD06_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN03705.1"
                     /translation="MPSIAPFRILVVDESDAFLASVVEWIASRRDLRLVGTARGGYEV
                     LNAVERLSPDLVILDAVLSGIDGFRLTRSIKKRAEAPLVVLLTFHASAAAREEALVAG
                     ADGFLAKQDFTDGFEALLAGWAEAGVGRTSRPTIPERPVRRGSRTEPDR"
     gene            complement(6020..6664)
                     /locus_tag="VFD06_08995"
     CDS             complement(6020..6664)
                     /locus_tag="VFD06_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN03706.1"
                     /translation="MKVLLADDHVLLRKGIRAVLASLPGIEVVAETADGREALSLIES
                     LRPDLAVVDITIPGLNGLEVAIRAKSVSPGTKVLILSMHAGEAYVAQALRAGVAGYLL
                     KEAADDDLPMAIKAVSRGDVYLSPKISRQVVERYVQSSSAAPDPLAGLTTRQREILQL
                     VAEGKSSKEIAVLLDLSVKTVESHRGQIMERLGVHDVTGLVRFAIRVGLVSPES"
     gene            complement(6661..>7388)
                     /locus_tag="VFD06_09000"
     CDS             complement(6661..>7388)
                     /locus_tag="VFD06_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZN03707.1"
                     /translation="KALLVVRASQEELHGAYARLRALTRQLEDAKEEEKRRIARELHD
                     EMGQILSAIKINLKALGRLPAGSQARSERIADSVQLVDDLIGHVREMALDLRPPLLDE
                     LGLVAALRGYAEGQSVRTGVAIGVEANADAEQVRPETAIAAFRIVQEAVHNTLRHASA
                     RQITVSVRKDGMRLLLSVRDDGRGFDVAEALGRAATGRHLGLLGMRERVEALGGRLEI
                     DSAPGQGAEIRASIPLTHEEGGV"
BASE COUNT         1135 a         2519 c         2490 g         1244 t
ORIGIN      
        1 ctcacccatt tgtcgccgcg accgtcccca tcggcgtcct ggtagtacgg cgtgtagact
       61 gcggggtccc cgtcattgca gtcggtggcg ttcgggatat acccgttcgg cggcgtcccg
      121 ccgctgcaga cgctgaccct cgtggaggca tctccgcggc cgtccccgtc cccgtcgatg
      181 ccgtaggtcg tcgccgggta gcagttcagg tcgacggtcg cgatcgcgcc cccttcggtg
      241 aagagagccc acacggcgac cctcccggtg tttccaagag cgtgcgagac gccgtccgtg
      301 ttggagaact ggacgtatcc ccctaccctg aacgtccgga aactgcccgg cacgccctcg
      361 atctcttcgc cggaccgtgc caccacgatc agccccttga tcggatcgaa gaccagcagc
      421 tctctgtcgt cgaccccccc catgatctcg ccccctcgca gattgccctg gatgaagatc
      481 tgcccgaggt cgttgaagga catggcccag ccgataaagg tgccgtaggt cgtctgcggc
      541 accggcacat ccgggatcgg cgggaacccc gggacggggg cgccggagcg cacgaccagg
      601 ctcagcgtgc cggacttcca tgtccagatg cccgagttgt tggtggggtc cgaggtgccg
      661 ccgttcaggg tggcctggaa cgcaacatct cccgcgtcgt tgatcaggct gtagaacggg
      721 ctgaacccct ggaagaatgc atcggtgccc ggggcagggt cgccggcgcg ggcgatcatg
      781 gtcagtccgc cgccgaccgc gccgacatac agcgccctgt cgttgacccc gtatgtgacg
      841 tcgccaccct tcaggtcgac cgccatctcg tacttcccgc tgcgcgtcag cgaggtgggt
      901 gaacagcccg gataccagtt gtcttgcaga ttgccgaacg tcgcgccgga ggtccccgga
      961 gccgggtccc cctcccgcac gagcagcgtg ctgcccgcgc cgggggtgta gaaccagagt
     1021 gaagaatcgt tggcggcggt gacgccggcc cccgagagcg tcacgtcgta gagcacccgg
     1081 ccgttgctgt ccatctgctg gatggtcccc atgccggccg ccctcaccgg gccgggcagg
     1141 accgcatccc ccagccgacg caggatcgac agcgccccgg gggtcccggt gaagatggcg
     1201 ccgttgttcc caccggtcgc atctccgccg atgagcgagc tctggaacag caccctcccg
     1261 gtgcggttga cctgagtgaa ttggtattcg gacttgaggt tgccggagaa cagcgctccc
     1321 accgtgcccg gcgcgacgtc cccctcccgc gcgaccagga ccgaaccgcc tggagagctg
     1381 cggaacaggg ccgtgtcatt gttcgtgttg acgcccgcgc cactcaggaa gctcgaccag
     1441 accttgtaac cgcccgacgg gctgagcaag gcggtgctcc cgatcccttg cgtcccggcg
     1501 ctgttgatga gcgcaaggcc cggaagtccc ggcgccggac cggaccagcg cgccaccatc
     1561 gaaaggtctg ccgcgctcgt gccggagaag agccccttgt aattggtgcc cgacacgccg
     1621 gcgccgtaca tgtccgattg gaagaagagg gtcccgtctt cggcgaccgt tggcaactgg
     1681 atgctgcccc cgaaatagac tcccggcagg cccggaacgt ccgtgtccgg ccaggcgaga
     1741 aagtcgccgt tggtcgtcat cagcgattgg gcgtgcgcgg gcgcgagcgc gcagaacatg
     1801 agcccgagga ccacggcgag acggatcggc gacgagcacg tcttcttcag atgtgtgttc
     1861 atctcttctc tcctaggtca cggacaggtg gacggtgacg cctgcatctc cgcatcgcgc
     1921 gaaccgactt gcgagggagc cccggaatcg aaggtcgcgc tgcctccgca gctgacggcg
     1981 cgcaccaggt accagaagcc gtcaccgacg ccgggcgcgt ccgcgtcatc gcgcgaggtg
     2041 gcggtcaggt cgctttcgag acagtcggtc gtcgccgcgc tgaaatcacc gtggctcgac
     2101 ctcagggccg acaagcttcc gcgcgccagg tcgtagccgg tggccgcggc gacgggactc
     2161 cacaccacct gcgcgatccc tttcgccggc tgtcccacgg tcagcgcggc gatccccgtc
     2221 ggggcggtcg cattgcactg gcagggcccg agcgtgtccc cggccgccag ggcggcatcc
     2281 aggccctggg ggaagctgac ggtcgtcgtc tggagctgcg tcggattgcc ggccggcgca
     2341 tggcagacga cggaggtcgg cgtcttcacg acgaagccga cgttcccgaa gcgggaagcg
     2401 agcgtccccg tgaggacgtg gccgtccgcc gacatcgccg tggcgccgag catgacggcg
     2461 tcagtgaggt tgaccccctg ggcgagcagg aaggtgttga aactggacca gtgcaatccg
     2521 gcggtccaga tggtcggcgt gaaggccccc gtcgtattgc tgctggtgta gccggtgatg
     2581 accctgtggt cgtcgctgat cgcgttggtg accgagttgt tgtagcccgt gaacagcccg
     2641 atcagctcga cgttcatggc gccgacggga tagcgccagg cgctgttcga cgtcgccggg
     2701 agcgagctct ggccgacgac gtactggccg tccctcgaca catcgacggc cgacaggacg
     2761 ctctggccat tcctggtgat cagcttgacg acgccgttct gccagtacgc gccgcgccag
     2821 aagccgctcg ttgtctcatc gaggccgacg atcaccgatc cgtcgtagtt gacgccgttg
     2881 gcgcgcgaca tgttgttgaa cgagctgaac ttcgggagcg caacgctccc gccggcggcc
     2941 gtccacttga acgcccggat ccccccgcgg aagcaggcgt cgccgtagga aagccccacc
     3001 acggtcgagc cgtcccccga gatgtcccag gcgctgttgt aggtcgtcgg tccgtcctgg
     3061 gtgcatgggg tcgaacccgg gaccggcggg agcagggtcc aggcgttgtt ctggtggatc
     3121 gcggccttca cggatccgtc cgtgtcgagg gcggtcgagg cgatcttcgt gccgtcgtcg
     3181 gagatggagg gggtccccac ccggaatccg ccgatgtcct cgaaggtgcc cgcatccacg
     3241 tcccaccgga agatcggccc gtccgtgttg ctcagcccgc ccacgacgat cgatccgtcg
     3301 ggcgtgatgt cggtgggttc gctgttgccc ggcgcggtca ggaagatggg cgcgccgggg
     3361 tagcggttga acgtgatgtt cgcggtggcg ggtgcgcccg gctgcgcggg caccgtcgtc
     3421 cacgcacagc ggtcgtcgtg cgccgaatcg ccgctttccg tcgctccgtt gaagtactcc
     3481 tccggaccgg gcagcacgat caccctcggc accgagaacg ccccgctcgc aaggttgtcc
     3541 gtgtagatca cgtaccgggc gcccggcgtc aggtcgttca gcacgaagga gccgtcgggc
     3601 ccggcctgcc ccttgctgac ctggcccgag atgtacgagc tgaaatcgtt gaagggatcg
     3661 aggacgttcc gcgcgatgac gttgacgccc gtgaccgggg ctcccgaggc atccaggatc
     3721 tggccgcgga tcgtcccccg gcttgcggga tagccgggag cggggtagag atccgacaat
     3781 gcggacatgt cgtcgagacg gtccacggtc cccatgtact cgccggcgtt ccccggctcg
     3841 ggggtgctga acgggtacat ggtctcgacc tgggtggcat ccgacacacc ggcccagggg
     3901 gcgttgcatc cttccggctg aggcaagtcg tacaccgccg ggaaccacac ggcgccgttc
     3961 gcctggctgt gcccgaggtt gagcgcgtgg cccatttcgt gggtgatgac gccctggaag
     4021 ccgactccgt ccggatcgtt cggatagatc cccggtccgc tgaggaccat ccacgcctcc
     4081 aggatctccg ggccgtcgtc tgcgacgtaa tcgatgctcg cgatcccgag cacaccttct
     4141 ggaggcagcc cgaagaagtt cgtcatgatg ctgccgtcgc tgtcgtagac gacgtggatg
     4201 ccgcccccgt tccacgcccc gaggaccgat tcgacattga cgtcgacaat gtcgtccagg
     4261 tcgagcgccg agaaatcgcc ggcgatcagt gctctgaagc tggaggtcgg gacgagggac
     4321 cactggtccg ccgcgaaaag cacctcctcg ctcgcccgcg cattgctcag gacggccagg
     4381 cttcccagat ccgtgtagac cggcacctcc ccgttgggcc acgtcgccat gttccaggcg
     4441 tacgggatgc gattctcgta gtcgtaggtg tagagggggc cgcccgcgaa ggcggcggtc
     4501 gcgacgccgg cgatcagcag cgccgcgagg agacatcccg cgaggaggga tcgatcgttt
     4561 cgtctcatcg gccgcccttc ggaagcgcga tactggcgtt cgggtcgagc ggcgccgtcg
     4621 gagcgttcgg ggcatcaggt ttgccggccg gtgtcggacg ctgggcatcg cccgggtcgg
     4681 atcgcggcgg ggtccggggg ccgccccgcc ccttggcgtg cgtcatccgg ccgttctcga
     4741 tccagcgctc ctcgaccgcg cgacgcacga aggagaggaa cgcgtcgggg ttgagcgggc
     4801 ccgcggcggt cgtcagcaac cgcttgtcgc gatcggtcac caggcccttg tcgaggtgga
     4861 catcgcggaa caggcccgcg ttgcccatgc cgttctccac gcggcccgcg tcgatggtga
     4921 acttgccgtg ggtggctccc gcgggcatgc ggaagccggt ccacgcagcc gccggacgca
     4981 ggaagaggag gacgttctct ccgggaacgt acttcgggaa ccctgcgggc cccggcatca
     5041 tctttctcct gccgtccgcg gtccgccgcg gattcagcag cccgaactgc cggaaggtgt
     5101 aggtctcggc gaggtcgccg cggatcgttt cagagatgtc gagggtgatc tcggtatagg
     5161 ggatcccgcg ctcgtcgatg ccgtccgtga ccgcctcgac tcttccaacg acgatcgcgt
     5221 tggcctctcc gagcaggtcc acgacattgg ccgacgtgag cgacatcgcc agcgcggttc
     5281 cgccgggcgc ggcccaggca agcgccaaca ggaccggcag agcgcgaacg cctgcgcgtc
     5341 cacccgtcat cagtcttcgc ccgattccca tccttctccc tcccatatcg cggacgcttc
     5401 ccggcgagtg cgcggaatgt acgcagagag ggacgccggg tacattgggg gaaactctga
     5461 tgcggcgacc ggagatcccc gggactcacc ccgacgccac ctcctgcggg gttaattcag
     5521 gggtttcagg gggtttacac cgatggattc ccaggttcgg acgcgagata ttcacggccg
     5581 cgttggctgc gccatctcgc actcgacgca aggagacccg tgccctcgat cgccccgttt
     5641 cgcatactgg tcgtggacga aagcgacgcc ttcctcgcaa gcgtcgtcga gtggatcgcc
     5701 tcgcgccggg atctccgcct cgtgggaacc gcccggggcg gctacgaagt tctcaatgcc
     5761 gtcgagcgcc tgtctccgga cctcgtgatc ctggacgccg tcctctcggg gatcgacggg
     5821 ttccgtctca cccggtcgat caagaagcga gccgaggcac ccctggtcgt cctcctcacg
     5881 ttccatgcga gcgctgcggc gcgcgaggaa gcgctcgtgg cgggagcgga cggcttcctc
     5941 gcgaagcagg acttcaccga tgggttcgag gcgctgcttg cggggtgggc ggaggcggga
     6001 gtcggtcgga cctcgcgccc tacgattccg gagagaccag tccgacgcgg atcgcgaacc
     6061 gaaccagacc ggtgacgtcg tggacgccga ggcgctccat gatctgtcca cggtgcgact
     6121 cgacggtctt cacgctgaga tcgagcagca cggcgatctc cttcgacgac ttcccttccg
     6181 ccacgagctg caggatctct ctctgccgcg tcgtcaggcc ggcgagggga tcgggcgccg
     6241 ccgacgagga ttggacgtac cgctcgacca cctggcggga gatcttcggg ctgagataga
     6301 cgtctccgcg ggacaccgcc ttgatcgcca tcgggagatc gtcgtcggcc gcctccttca
     6361 ggaggtaacc cgcgaccccg gcgcgcagcg cctgagccac gtaggcctcg ccggcgtgca
     6421 tcgacaggat cagaaccttc gttccgggag acacgctctt agccctgatc gccacctcga
     6481 gaccgttcag accgggaatc gtgatgtcga cgaccgccag gtcgggacgg agactctcga
     6541 tgagagacag tgcctcacgg ccatccgctg tttccgcgac gacctcgatg ccgggcagcg
     6601 aagccaggac cgcccggatc ccctttcgca ggaggacgtg atcgtctgcg agcaggacct
     6661 tcatacgccc ccctcctcgt gggtcaaggg gatgctcgcg cggatctccg ctccctgtcc
     6721 gggtgcggaa tcgatttcca gacgcccgcc gagcgcctcc acgcgctcgc gcatgccgag
     6781 gaggcccagg tgccggcccg tcgccgcgcg cccgagcgcc tccgcgacgt cgaagccgcg
     6841 cccatcgtcg cgcacgctga ggagcagtcg catcccgtcc tttcgcaccg acacggtgat
     6901 ctggcgcgcc gaagcatgcc gcagcgtatt gtgcaccgct tcctggacga tccggaaggc
     6961 ggcgatggcg gtttcgggac ggacctgctc cgcgtcggcg ttcgcctcga cgccgatcgc
     7021 cacgccggtg cggacggact gaccctcggc gtacccgcgg agcgcggcga cgaggcccag
     7081 ctcatcgagc aggggcgggc ggagatcgag cgccatctca cggacgtgtc cgatcaggtc
     7141 gtccacgagc tggacggagt cggcgatccg ctcgctccgc gcctgggagc ctgccggcag
     7201 ccggccgagc gctttcaggt tgattttgat ggcgctcagg atctgaccca tctcgtcgtg
     7261 cagctccctc gcgatccggc gcttctcttc ctccttggcg tcctccagtt gccgtgtcag
     7321 cgcgcggagc cgggcgtacg caccatgaag ctcctcttga ctggctcgca ccacgaggag
     7381 cgccttct
//
LOCUS       DASRIQ010000373         7298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_398603, whole genome shotgun sequence.
ACCESSION   DASRIQ010000373 DASRIQ010000000
VERSION     DASRIQ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398603"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1225)
                     /locus_tag="VFD06_09005"
     CDS             complement(23..1225)
                     /locus_tag="VFD06_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN03708.1"
                     /translation="MRHVRSLNIPLLAVAVWAACALTAAPARAADPLQGLLADGDEAT
                     RREAIRERVRALGDAACPTLSAYAGAPDPRAREHAVEAMEEAGCDRLEDYRDFFRDRS
                     AWVVRAVARALGRHRIAGGVPFLLSHLEDRRTLVSDGDSVTLSDVVEQALRRLTAQPI
                     EPEGAAPRQAPGGREAAVAGAWRAWYAAHAQEPPARWLSDGRTTIRAALAGGSPPARY
                     AALQTLALVGEPVVDLLLEALQRRAGEITAALQCAPDEPPRVTEEVPCTLSLRNDTAR
                     RIPLALGEIDLRLGPATAAPAPAPAKPPAGKEIKKDNPPKGAAKPAAPAPPPVPILEP
                     ERLAGTIVDLAPGQVLRRPVSVGPVPAAGRYEAVAAFQDLGRRLKPGAFPSDLPRLEA
                     TLLLRFEQ"
     gene            1478..1951
                     /locus_tag="VFD06_09010"
     CDS             1478..1951
                     /locus_tag="VFD06_09010"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZN03709.1"
                     /translation="MNETPGSLVTIPEPKTAAPRTTGVAVIGSSIQIKGELQGDEDLT
                     IDGRVEGKIDLRDHNLTIGPNGKIKADLFARHIVIAGDVSGNAHAAERVEIAPSGRLT
                     GDIVSPRITIADGAHFKGSVDMEHKGAAKAPAREEARGLGIAASRDDSAKSQQRL"
     gene            2015..3001
                     /locus_tag="VFD06_09015"
     CDS             2015..3001
                     /locus_tag="VFD06_09015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN03710.1"
                     /translation="MLDRLLRQRSRPPDGPDDPSESLRSHVFPRFLKWLSGRDHPSVL
                     DLGRLSGGNIEIFARLGCRVQVEDLLAAAEEKIRPAAGEPTPTAEPMSAAPLPQVAES
                     APAPAAGASAAAPIEGPAAPAASASGPNGQGPTAPDATPATGRPTRHIVLPPRVFSRT
                     TVAGTNGTNGGPAQRSYTPRQALVTLPLPTSFGYPEASFDAIVGWDLFNYYDPDTARR
                     MGPDLVRLLRPGGLVFATFHARREEAPDGPARHRIVDESHLQVAPHAGPPRARHLYQN
                     RDIEKMFAGLKIVELYFLRHGVREMLLEKRPGSGAPPAIGGPARPRPRFRIE"
     gene            3011..4870
                     /gene="typA"
                     /locus_tag="VFD06_09020"
     CDS             3011..4870
                     /gene="typA"
                     /locus_tag="VFD06_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZN03711.1"
                     /translation="MNGRRDLRNIAIIAHVDHGKTTLVDRLLQQTGTFRSNERVQERV
                     MDSNDLERERGITILAKNTSVRFEGVKINIVDTPGHADFGGEVERVLGMVDGALLLVD
                     AAEGPLPQTRFVLRKSLENGHRPIVVINKVDRPDARPHQVLDEVFQLMLNLGATDEQL
                     DFPHLYASAKLGWAVREMEEAPQGTAPAPEGIAPLLRTILEKVPAPPGDAAGPAQLQI
                     ANLDYNDYVGRMAIGRLSRGALRWGMPIAILKLDGRTEAHKVTRLETFEGLRRVEVQE
                     AAAGEIVALAGIPDIQIGETIADPEHPDPLPPIRVEEPTLAMEFMVNDSPFAGREGRF
                     VTSRHLRDRLRKEALANVALRVDETATTDTFTVSGRGELHLAIVAETMRREGYEFQLS
                     RPQVILKRDEKGAALEPIEYLVLDLEEAYTGRVMEMLGERRAILANMAGAGTGRVRLE
                     FTVPARGLLGFRREYLTETRGTGILSHVFHSYGPWRGDIADRHRGALVVKEPGTTVTF
                     ALYNLEDRGTLFLGPGIEVYEGMIVGEHTRENDLVVNPCKRKHLTNMRASTSDETLRL
                     KPPRLMSLEDGIEFLADDELVEVTPKTVRLRKRSLRSSDRKRSEKAAAEAVAP"
     gene            4905..5699
                     /locus_tag="VFD06_09025"
     CDS             4905..5699
                     /locus_tag="VFD06_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZN03712.1"
                     /translation="MIGARGRRGARPLVLAHRGSRLRAPENTLEAFALALQEGADGIE
                     LDVRRTADGVPVVLHDPALDRTTDGRGPLAVTTFADLRRLDAGAWFDRRHRGAHVPSL
                     AEVLDLCGGRAVVNIELKIDSGRGLAARRAAIAEAPRLAEAVALDLARARRGGFVLVS
                     SFSARALQAAHAVMRNVPLGWLRSRSTRGLAALNRRLRLHSVNPNLRLASARRFAAAR
                     RLGLSVYVYPVNVPGDITRLARLGATGLITDDPLVALSTLQHHRDV"
     gene            complement(5827..6129)
                     /locus_tag="VFD06_09030"
     CDS             complement(5827..6129)
                     /locus_tag="VFD06_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03713.1"
                     /translation="MPYVGAPPPMGLSDSDSDDEVGIGVTAAAGPAGFGVFFGADRAG
                     DARRAVLGRAAFFVDFPELLRRGLALLDDALRAGLLRLGALRFAFFLATVVPPWFV"
     gene            6316..6825
                     /locus_tag="VFD06_09035"
     CDS             6316..6825
                     /locus_tag="VFD06_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03714.1"
                     /translation="MPALDPPANSEEAFKRGLACLERRSYQEAAVCFQYSIDLDSRPG
                     QQSQSNMRYVSFLGLALNLAQVRSDEGLKMCEQAAKREFFDPDVFCNLGIAYLRHRQR
                     GPAFSAFHRGLALRPKHRRILEELDRYDRRSAPVFPFLARQHTLNILGGRLRTRLRAL
                     LGRLSPNAA"
     gene            6912..>7298
                     /locus_tag="VFD06_09040"
     CDS             6912..>7298
                     /locus_tag="VFD06_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03715.1"
                     /translation="MLKASPESLVARGLLLVDRATAADHQEARRLFRSALKANANLPL
                     AHAGLARSATFLYSAGIDESRERLEEALSASERAAALAPDDAAIRTVRALALAAADRL
                     TPALAEAERAAALAPDREDGPLALCIV"
BASE COUNT         1060 a         2699 c         2528 g         1011 t
ORIGIN      
        1 cggggcgctg ttttcggagg atttactgct cgaacctcaa gagcaacgtg gcttccaggc
       61 gtggcagatc ggacggaaag gcgcctggtt tgagcctcct tccgaggtcc tggaaggcgg
      121 cgaccgcctc gtagcggccg gccgccggga ccggcccgac gctcaccgga cgcctcagga
      181 cctggccggg agccagatcg acgatggttc cggccagccg ctccggctcc aggatgggca
      241 ccgggggtgg tgcgggagcc gccggcttcg ccgctccctt cggcgggttg tccttcttga
      301 tctcttttcc tgccggcggc ttcgcggggg cgggggccgg tgcggccgtc gccggaccga
      361 gccgcagatc gatctccccc agcgcgagcg ggatgcggcg cgcggtgtcg ttgcgcagcg
      421 acagcgtgca gggaacttct tcggtgaccc gcggcggctc gtccggggcg cactgcagtg
      481 ccgcggtgat ctccccggcg cgccgctgga gcgcctccag gagcagatcg acgaccggct
      541 cgccgacgag ggccagcgtc tggagcgcgg cgtaacgggc cggcggggac ccacccgcca
      601 gcgccgcgcg gatggtggtg cgtccgtcgc tcagccagcg ggccgggggc tcctgcgcgt
      661 gcgccgcgta ccaggcgcgc caggcgccgg cgaccgccgc ctcgcgcccg ccgggcgcct
      721 ggcgcggcgc ggccccctcc ggctcgatcg gctgagccgt caggcgccgg agcgcctgct
      781 cgaccacgtc ggagagtgtg acgctgtccc cgtccgagac gagcgtccgg cgatcctcga
      841 ggtgcgacag caggaacggc acgccccccg cgatccggtg gcggccgagc gcccgcgcca
      901 cggcccgcac cacccacgcc gatcgatcgc ggaaaaaatc gcgatagtcc tccagccggt
      961 cgcagccggc ctcctccatc gcctcgacgg cgtgctcgcg cgcccgcggg tcgggggcgc
     1021 cggcgtaggc ggacagcgtc gggcaggcgg cgtcgccgag ggcccggacc cgctcgcgga
     1081 tcgcctcgcg gcgcgtcgcc tcgtcgccat cggcgagcag cccctggagc ggatcggcgg
     1141 cgcgagccgg cgcggccgtg agggcacagg cggcccagac cgcgacggca agcagcggta
     1201 tgttgaggct tcgaacgtgg cgcacggcgc acattctagc ggctccttgc cttgaccgtc
     1261 tcagatccct atgttagagt ccgcgccata tttttggagc gagatcggat cgcatttcgg
     1321 tttctccctg atccgatcgg aaccggcccc ccatcggcgc gtttttcatc ttcggggccg
     1381 gatccacgcc gatcacgccg gcgtcgctga tgctcccgtt tcctttcttg gaggaggtcg
     1441 aggaatgtgg gacaagaagg atcctgccgc acgccccgtg aatgagacgc ccggaagcct
     1501 cgtgaccatc ccggagccga agaccgccgc gccccgtacg accggcgtcg ccgtcatcgg
     1561 ctcctcgatc cagatcaagg gagagctcca gggagacgag gacctgacga tcgacggccg
     1621 ggtggagggg aagatcgacc tccgcgacca caacctgacg atcggcccga atggcaagat
     1681 caaggccgac ctcttcgcgc gccacatcgt catcgccggt gacgtctccg gcaacgcaca
     1741 cgccgccgag cgggtcgaga tcgccccgtc cggccgcctg accggcgaca tcgtgtcgcc
     1801 gcggatcacg atcgccgacg gcgcccactt caagggcagc gtcgacatgg agcacaaagg
     1861 cgccgccaaa gcgccggcgc gcgaggaagc gcgcggcctg gggatcgccg cgtcgcgcga
     1921 cgactccgcc aaatcccagc agcgtctcta gtcttcgatt cgcggccccg gtccgccggt
     1981 cccaccggcg ggccggcgcc gcggacatcg ctccatgctc gatcgcctcc tgcgccagag
     2041 gtcccggccc cccgacgggc cggatgatcc gagcgaatcg cttcgctcgc acgtcttccc
     2101 tcgtttcctg aagtggctgt cggggcgcga tcacccctcc gtgctcgatc tcggccggct
     2161 ctcggggggc aacatcgaga tcttcgcccg cctcggctgc cgcgtgcagg tcgaggatct
     2221 gctggccgcc gccgaggaga agatccggcc cgccgcaggc gagccgacac cgacggccga
     2281 gccgatgtcg gcggccccgc tcccgcaggt cgccgagtct gcgcccgcgc cggccgccgg
     2341 ggcgtccgca gccgccccga tcgaaggccc agccgcgccg gccgcgtccg cctccggccc
     2401 gaacggccaa ggccccaccg ccccggatgc gaccccggcg accgggcggc ccacgcgaca
     2461 catcgtcctg ccgccgcgcg tcttctcgcg caccaccgtc gccgggacga acgggacgaa
     2521 cggcgggccg gcgcagcgtt cgtacactcc gcggcaggcg ctggtgactc tgccgctgcc
     2581 gacctcgttc ggctatcccg aagcgtcgtt cgacgcgatc gtcggatggg acctcttcaa
     2641 ttactacgac ccggacaccg cccggcggat ggggccggat ctggtgcgcc tgctgcgtcc
     2701 cggcgggctc gtgttcgcga ccttccatgc ccggcgcgag gaggcgccgg acgggccggc
     2761 gcggcaccgc atcgtggacg agtcgcacct gcaggtggcg ccgcacgccg ggcccccccg
     2821 ggcgcgccac ctctaccaga accgggacat cgagaagatg ttcgccgggc tgaagatcgt
     2881 cgagctgtac ttcctgcgtc acggggtccg cgagatgctg ctcgagaagc gtccgggcag
     2941 cggcgcgcca cccgccatcg gagggccggc ccgcccgcga cctcgattcc gtatcgagta
     3001 aagtacgtcc gtgaacggcc gacgggatct ccgaaacatc gccatcatcg cgcacgtcga
     3061 tcacggcaag acgacgctcg tcgatcgcct gctccagcag accggcacct tccgctccaa
     3121 cgagcgggtg caggagcggg tcatggacag caacgacctg gagcgcgagc gcggcatcac
     3181 catcctggcg aagaacacct cggtgcgctt cgaaggggtc aagatcaaca tcgtcgacac
     3241 ccctggccac gccgacttcg gcggggaggt ggagcgggtc ctcggaatgg tggacggggc
     3301 gctgctcctc gtcgacgccg ccgaggggcc gctcccacag acccgtttcg tgctgcgcaa
     3361 gtcgctcgag aacggccacc ggccgatcgt cgtgatcaac aaggtggacc ggcccgacgc
     3421 gcggccgcac caggtgctcg acgaggtgtt ccagctgatg ctgaacctcg gcgccacgga
     3481 cgagcagctc gatttcccgc acctctatgc ctcggccaag ctcggctggg cggtgcggga
     3541 gatggaggag gcgccgcagg ggacggcgcc cgccccggag gggatcgcgc cgctcctgcg
     3601 caccatcctc gagaaggtcc ccgccccgcc cggcgacgcc gccggaccgg cccagctgca
     3661 gatcgccaat ctcgattaca acgactacgt cgggcgcatg gcgatcggcc gactctcccg
     3721 cggcgccctg cgctggggga tgccgatcgc gattctgaag ctcgacgggc ggaccgaggc
     3781 gcacaaggtg actcggctcg agaccttcga ggggctgcgg cgggtggagg tgcaggaggc
     3841 ggcggcgggg gaaatcgttg ccctcgccgg tattcccgac atccagatcg gcgagacgat
     3901 cgccgacccg gagcatcccg atccgctgcc gccgatccgc gtcgaggagc cgacgctcgc
     3961 gatggagttc atggtgaacg actcgccgtt cgccgggcgc gaggggcgct tcgtcacctc
     4021 ccgccacctg cgcgatcgcc tccgcaagga ggcgctcgcg aacgtcgcgc tgcgcgtcga
     4081 cgagacggcg acgaccgaca ccttcacggt ctccgggcgc ggcgagctgc acctggcgat
     4141 cgtcgccgag acgatgcgcc gcgaagggta cgagttccag ctgtcgcgac ctcaggtgat
     4201 cctcaagcgc gacgagaagg gggcggcgct cgagccgatc gagtacctgg tcctcgatct
     4261 cgaggaggcg tacaccggcc gggtgatgga gatgctcggt gagcggcgcg ccatcctcgc
     4321 gaacatggcg ggggcgggca ccggccgggt ccgactggag ttcacggtcc cggcccgcgg
     4381 cctcctcggg ttccgccgcg agtacctgac cgagacgcgg ggcaccggta tcctgtccca
     4441 cgtcttccat tcctacggac cgtggcgcgg cgacatcgcg gatcggcacc gcggggcgct
     4501 cgtggtgaag gagccgggca cgacggtgac gttcgccctc tacaacctcg aggatcgcgg
     4561 caccctgttc ctgggacccg ggatcgaggt gtacgagggg atgatcgtcg gcgagcacac
     4621 gcgcgaaaac gacctcgtgg tcaatccctg caagcgcaag cacctgacga acatgcgcgc
     4681 ctcgacctcc gacgagaccc tgcggctgaa gccgccgcga ctcatgtcgc tcgaggacgg
     4741 catcgagttc ctcgccgacg acgagctggt ggaggtgacg ccgaagacgg tgcgcctgcg
     4801 caagcgctcg ctccgatcga gcgatcggaa acgcagcgag aaggccgctg ccgaggcggt
     4861 cgccccatga ccgacggccc ggacgccggc gacgacctcc tgacgtgatc ggcgcgcgcg
     4921 gccgccgtgg cgcgcgcccg ctcgttttgg cgcaccgcgg ctcccggctg cgcgccccgg
     4981 agaacaccct cgaggccttc gcgctggcgc tccaggaggg ggcggacggg atcgagctcg
     5041 acgtgcggcg caccgccgac ggcgtgccgg tggtactgca cgatcccgcg ctcgatcgca
     5101 ccaccgacgg gcgcgggccg ctggccgtga cgacgttcgc cgatctccgc cggctcgacg
     5161 cgggcgcctg gttcgatcgg cggcatcgcg gtgcccatgt tccttcgctc gccgaggttc
     5221 tcgacctgtg cgggggccgc gccgtcgtga acatcgagct caagatcgac tccggccggg
     5281 gacttgcggc gcggcgcgcg gcgatcgccg aggccccgcg tctcgcggag gcggtggcgc
     5341 tcgatctcgc gcgcgcccgt cgcggcggat tcgtgctcgt ctcatcgttc tcggcgcggg
     5401 cgctgcaggc ggcgcacgcc gtcatgcgga acgtcccgct cggctggctg cgctcccgat
     5461 ccacgcgagg tctcgccgcc ctgaaccgcc ggctgcggct gcactccgtg aacccgaacc
     5521 tgcgcctcgc ctcggcccgc cgcttcgccg ccgcgcgccg cctcggcctg tcggtctacg
     5581 tctaccccgt caacgtcccg ggcgacatca cccgcctcgc acgcctcggc gccaccggcc
     5641 tcatcaccga cgaccccctc gtcgcccttt cgaccctcca gcaccaccgc gacgtctgag
     5701 caagctcgtc cgttcgccgt gaagatcgga tcgtccggag cccccgcgcg cgggggctcg
     5761 ggacgatccg ccgtcacttg acgaatgtga gcgcttgtga tgaattacct cgctgatccc
     5821 aagcactcaa acgaaccaag ggggtacgac tgtggccaag aagaaagcga agcgcaaggc
     5881 gccgagacgc agcaagccgg cccgcaaggc atcgtccagg agggccagcc cccgccgcaa
     5941 gagctccggg aagtcgacga agaaggcggc ccgtccgagc acggcccggc gcgcgtctcc
     6001 cgcgcggtcg gccccgaaaa agacgccgaa accggccggc ccggctgcgg cggtgacccc
     6061 gatgccgacc tcgtcatccg aatccgagtc ggataagccc atcggcggag gggctccgac
     6121 gtacggcatg ggctggggtt ccgaagagga gtagaaccgc gcctcctgct ctggcgcgtc
     6181 gcatccgagg gccgtccccc gtccggaaac gggcgcggga cggccctttt agcttccggg
     6241 atggttgacg gagacggggg ccggcttata ttggggccat gtcgaacacg gaactcgtga
     6301 cgcgcccgag gagagatgcc cgctctcgat ccgccggcca attcggaaga agcgttcaag
     6361 cgcgggctcg cctgcctgga gcgccgctcc taccaggaag cggcggtctg cttccagtac
     6421 tcgatcgacc tggactcccg tcccgggcag caaagccaat cgaacatgag gtacgtgtcg
     6481 ttcctggggc tggcgctgaa tctcgcccag gtccgctcgg acgaagggct caagatgtgc
     6541 gagcaggcgg cgaagcgcga gttcttcgac ccggacgtct tctgcaacct cggcatcgcg
     6601 tacctgcggc accggcagcg cggcccggcc ttctcggcct tccaccgcgg tctggcgctg
     6661 cgcccgaagc accggcgcat cctggaggag ctcgaccgct acgaccggcg ctcggcgccg
     6721 gtcttcccgt tcctggcgcg ccagcacacg ctgaacatcc tgggaggacg cctgcggacc
     6781 cgcctgcgcg ccctcctcgg ccgtctctcc ccgaacgcag cctgacccgg gcggttccgc
     6841 tcctcctggc gctcgccgcg agcgccccgg ccacgttcgc cgcccccgaa cctgtgcccg
     6901 ggtcgcgcga gatgctcaag gcgagcccgg agtcgctcgt cgcccgcggc ctgctgctcg
     6961 tcgatcgcgc caccgccgcc gatcaccagg aggcgcggcg gctgttccgg agcgcgctga
     7021 aagccaatgc gaatctcccc ctcgcccacg ccggtctggc ccgatcggcg acgttcctgt
     7081 actcggcggg gatcgacgaa tcccgggagc gcctcgaaga ggcgctcagc gcctccgagc
     7141 gcgccgccgc cctggcgccg gacgacgcgg cgatccgcac ggtccgcgct ctggcgctcg
     7201 ccgccgccga ccggctgacc cccgccctcg ccgaggccga acgggcggcg gctctcgccc
     7261 cggatcgcga ggacgggccc ctggcgctct gcatcgtc
//
LOCUS       DASRIQ010000374         4059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_683606, whole genome shotgun sequence.
ACCESSION   DASRIQ010000374 DASRIQ010000000
VERSION     DASRIQ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683606"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /gene="pyrH"
                     /locus_tag="VFD06_09045"
     CDS             <1..241
                     /gene="pyrH"
                     /locus_tag="VFD06_09045"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008308239.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZN03716.1"
                     /translation="KATQVDGVYSADPKKDAAAERYEEITYRQVLSDGLRIMDASAIA
                     LCQENKVPIMVFSLSVPGNILRAAQGEPIGTLVKE"
     gene            245..805
                     /gene="frr"
                     /locus_tag="VFD06_09050"
     CDS             245..805
                     /gene="frr"
                     /locus_tag="VFD06_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZN03717.1"
                     /translation="MALDASIETARKKMHDSVEHTRKDLAAVRTGRASLALLDGITVE
                     YYGTETPLAQVAGLSIPDPTLIVAQPWDATLIPKIEKSIRAANLGLNPANDGKVVRIP
                     VPALTEERRKQLVKKVHEIAEHGRTAIRSERREANEVLKKLLKDHKISEDDERRGLDQ
                     VQKLTDQHVKQIDEMSKHKEDELLKI"
     gene            813..1496
                     /gene="ispD"
                     /locus_tag="VFD06_09055"
     CDS             813..1496
                     /gene="ispD"
                     /locus_tag="VFD06_09055"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZN03718.1"
                     /translation="MRIGLVLAAAGLGTRLKASEPKAFLRLQDATLVDRALAPFMLRD
                     DVAAAAVVVPNPAAAAELLGHMDPRVRFVQGGEDRQASVAAGLAVLGDVELILVHDAA
                     RPLVEDATVAAVIDAAKKFGAAVPALPIHDTVKEVGADGRVKGTPSRAALVLVQTPQG
                     FRADLLRRAHAEAQAAGVRATDDAALVERLGEPVVLVPGRRSNIKITTREDLLIAEAI
                     LAARAQPPR"
     gene            1493..2056
                     /gene="ispF"
                     /locus_tag="VFD06_09060"
     CDS             1493..2056
                     /gene="ispF"
                     /locus_tag="VFD06_09060"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZN03719.1"
                     /translation="MSRRPGPAKGGPPRIGLGIDRHPFEAGRRLVLGGIIIPDADGLR
                     GHSDADVLTHAVCDALLGALGLPDLGTRFPSTSEQWRDKKSLLFLDDIMQELWQRRYV
                     VGNLDAVLLAERPPLLPHLPSMRSLLAKRLHCEPERVSIKPKRGEGLGFIGRGEGIEA
                     HAAVMLVKGTPPKRVDPVAERRRRSAR"
     gene            2053..3570
                     /gene="gltX"
                     /locus_tag="VFD06_09065"
     CDS             2053..3570
                     /gene="gltX"
                     /locus_tag="VFD06_09065"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZN03720.1"
                     /translation="MSDGAVRIRFAPSPTGDLHVGNARTALFNWLFARHAGGRFILRI
                     EDTDRERSRPEHERQILADLRWLGLGWDEGMDEGGDGGPYRQSERLPLYREAADRLLG
                     AGAAYRCFCAPALLEEERAREREAGRASLYPGRCRGMAREESDARARGGEAAAIRFDV
                     RAAARGDDAVGFEDLVHGTVRVPLDQIGDFVLVRRDGGPAYNFAVVVDDAAMRITHVI
                     RGDDHLSNTPRQILVARALGVASPRFAHLPLLVAPGGAPLSKREGAVSVRALRDAGYP
                     PEAVLNHLALLGWSPPGGVEVLTREELVAAFDLARVSRSPAVFDRQKLDAIAMRHLQR
                     WDPVRLAAEAAPVLQEAGLLPERLTGDVTAWSESVVGLVSDRLSRLGELPGLLGVVFG
                     FDLETSLRDEDVRGTLGDSRGRQVVSETLGLLEEEPLTAPRLQTIVAEVRRRTGAKGK
                     DLFHPIRVGLTGFSSGPELVRLLPVLDAGSRLDLPRPVPGCADRARSLLAATKKA"
     gene            3609..>4059
                     /locus_tag="VFD06_09070"
     CDS             3609..>4059
                     /locus_tag="VFD06_09070"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HZN03721.1"
                     /translation="MSQWIFGLHAVLEAIERTPDRVTRIVVAQGREEGRLRLLMAAAR
                     RAGVAVRREPTAAIDRLAGKGAVHQGVLALLAEAPYADPEHLLASAPSPALFVVLDGV
                     EDPHNLGAVIRSAVAAGAHALFLPEHHSAGLSAGAVKASAGTALRFPV"
BASE COUNT          587 a         1459 c         1449 g          564 t
ORIGIN      
        1 caaggcgacg caggtggacg gcgtctacag cgccgacccg aagaaggacg ccgcggccga
       61 gcgctacgag gagatcacct accgccaggt cctgtcggac ggcctgcgga tcatggacgc
      121 gtccgccatc gcgctgtgcc aggagaacaa agtgccgatc atggtgttca gcctgagcgt
      181 tccgggcaac atcctgcggg ccgcccaggg ggagccgatc ggcaccctcg tcaaggagtg
      241 actcatggcc ctcgacgcct cgatcgaaac cgcccggaag aagatgcacg actcggtgga
      301 gcacacgcgc aaggatctgg ccgcggtccg cacggggcgc gcgtcgctcg cgctgctcga
      361 cggcatcact gtcgagtact acggcaccga gacgccgctc gcgcaggtcg ccggcctcag
      421 catcccggac ccgaccctca tcgtcgcgca gccctgggac gccaccctga tcccgaagat
      481 cgagaaatcc atccgcgccg ccaacctcgg gctcaatccg gcgaacgacg gcaaggtggt
      541 gcggatcccg gtgccggctt tgaccgagga acgccgcaag cagctggtga agaaggtgca
      601 cgagatcgcc gagcacggcc gcaccgccat ccgcagcgag cgtcgcgagg cgaacgaggt
      661 cctcaagaag ctcctcaagg accacaagat ctccgaggac gacgagcgcc gcggcctcga
      721 ccaggtgcag aagctcaccg atcagcacgt caagcagatc gacgagatgt cgaagcacaa
      781 ggaagacgag ctcctcaaga tctgacgacc ccttgagaat cgggctggtc ctcgccgccg
      841 ccgggctcgg gacgcgactc aaggcctccg agccgaaagc cttcctgcgt cttcaggacg
      901 cgacgctcgt cgatcgtgcc ctcgcgccgt tcatgctgcg tgacgatgtg gccgccgccg
      961 ccgtggtggt gccgaatccg gccgccgccg ccgagctgct cggccacatg gacccgcgcg
     1021 tccgcttcgt ccagggaggc gaagaccggc aggcttcggt cgccgccggc ctcgccgtcc
     1081 tcggggacgt ggagctcatc ctggtccatg acgccgcgcg cccgctcgtc gaggacgcca
     1141 cggtggccgc ggtgatcgat gcggcgaaga agttcggggc ggcggtgccg gccctgccga
     1201 tccacgacac agtgaaggaa gtgggagcgg acgggcgcgt caaggggacc ccatcccgcg
     1261 ccgcgctggt gctggtccag actccccagg ggttccgcgc cgatctgctg cgccgggcgc
     1321 acgccgaggc gcaggcggcc ggcgtgcgcg ccaccgacga cgcggccctc gtcgagcgcc
     1381 tcggggagcc ggtcgtgctc gtccccgggc gccgctccaa catcaagatc acgacgcgcg
     1441 aggatctgct gatcgccgag gcgatcctcg ccgcccgcgc acagccgccg cgatgagccg
     1501 gcgccccggc ccggcgaagg gcgggcctcc gcgcatcggt ctggggatcg atcgccatcc
     1561 cttcgaagcg ggccggcggc tggtgctcgg ggggatcatc attcccgacg ccgacggcct
     1621 ccgcggccat tccgacgccg acgtcctgac gcacgccgtc tgcgatgccc tgctcggagc
     1681 gctcggcctg cccgacctgg ggacccggtt cccctccacc tcggagcagt ggcgcgacaa
     1741 gaaaagtctt ctcttcctgg acgacatcat gcaggagctg tggcagcggc ggtacgtcgt
     1801 agggaatctc gatgcggtcc ttcttgccga gcgcccaccc ctgctgccgc atctcccctc
     1861 catgcgaagc cttctcgcaa agaggctcca ctgtgagccg gaacgtgtct cgatcaagcc
     1921 gaagcgaggg gagggcctcg gtttcatcgg acggggagaa ggaatcgaag cgcacgccgc
     1981 cgtgatgctg gtcaagggaa cgcccccgaa gcgagtcgac ccggtcgccg aaaggcggcg
     2041 ccgctcggcc cgatgagcga cggcgcggtc cggatccgct tcgcgccgtc gccgaccgga
     2101 gacctgcacg tcggcaacgc ccggaccgcg ctgttcaact ggctcttcgc gcggcacgcc
     2161 ggaggtcgat tcatcctgcg catcgaggac accgaccggg agcgctcccg gccggagcac
     2221 gagcggcaga tcctcgccga cctgcgctgg ctcggcctcg gctgggacga ggggatggac
     2281 gaaggcggcg acggcggccc gtaccgccag tcggagcgcc tgccgctcta tcgcgaggcc
     2341 gccgatcggc tgctcggcgc cggggccgcc taccgctgct tctgcgcccc cgcgctcctc
     2401 gaggaggagc gggcgcgcga acgggaggcg gggcgcgcct cgctgtatcc gggacgctgc
     2461 cgcgggatgg cccgcgagga gtcggacgcg cgcgcccggg gcggcgaggc ggcggcgatc
     2521 cgcttcgacg tgcgcgccgc cgcgcgcggc gacgacgccg tgggcttcga ggatctcgtc
     2581 cacgggacgg tgcgggtgcc gctcgaccag atcggcgact tcgtcctcgt tcgccgcgac
     2641 ggcggcccgg cctacaactt cgccgtcgtg gtggacgacg ccgccatgcg gatcacccac
     2701 gtcatccgcg gcgacgatca cctgtcgaac accccgcgcc agatcctggt cgcccgggcg
     2761 ctcggggtcg cgtcgccgcg attcgcgcac ctgccgctcc tggtcgcccc cggcggcgcg
     2821 ccgctgagca agcgggaggg ggcggtctcc gtgcgggcgc tgcgcgacgc cgggtacccg
     2881 cccgaggcgg tgctcaacca tctcgcgctg ctcggctggt cccctccggg aggcgtggag
     2941 gtgctgacgc gcgaggagct ggtcgccgcc ttcgatcttg cccgcgtctc ccggtcgccg
     3001 gccgtcttcg atcgacagaa gctcgacgcg atcgcgatgc gccacctgca gcgctgggac
     3061 ccggtccgcc tggcagcgga ggcggccccg gtcctgcagg aggcggggct cttgccggag
     3121 cggctcaccg gggacgtcac cgcctggagc gagtcggtcg tggggctggt ctcggaccgt
     3181 ctgtcgcggc tcggcgagct tccgggactt ctcggtgtcg tcttcgggtt cgacctcgag
     3241 acctcgcttc gggacgagga cgtgcgcggg accctcggcg actctcgcgg ccggcaggtg
     3301 gtgtccgaga cactcggtct cctcgaggag gagccgctca ccgcgccgcg tctgcagacc
     3361 atcgtcgccg aggtccggcg ccggaccggg gcgaagggga aggacctgtt ccacccgatc
     3421 cgggtcgggc tgacagggtt cagttcgggc ccggagctcg tgcgccttct cccggtgctc
     3481 gacgcgggga gccggctcga cctgccccgt ccggtgcccg gctgcgcgga tcgggccaga
     3541 agcctcctcg ccgccacgaa gaaggcatga atgatacgcg gcgggtgcag gatcggagag
     3601 gaaggagcgt gagccagtgg attttcggcc tgcacgccgt cctcgaggcg atcgagcgca
     3661 ctcctgaccg ggtcacccgc atcgtcgtcg cacagggaag ggaggagggg aggctccgtc
     3721 tgctcatggc cgcggcccgg cgggcaggcg tggcggtgcg gcgcgaaccc acggcggcga
     3781 tcgaccgcct cgccgggaag ggggccgtgc accagggggt gctggcactt ctcgccgaag
     3841 ccccctacgc cgacccggaa caccttctcg cgtccgcccc gtcgcccgcc ctcttcgtcg
     3901 tcctcgacgg ggtggaggac ccccacaacc ttggagcggt catccgctcc gcggtcgcgg
     3961 ccggggcgca tgcgctgttc ctccccgagc accattcggc cgggctctct gcaggcgccg
     4021 tgaaggcttc ggcggggact gccttgcggt ttccggtgg
//
LOCUS       DASRIQ010000375         4156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2480531, whole genome shotgun sequence.
ACCESSION   DASRIQ010000375 DASRIQ010000000
VERSION     DASRIQ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2480531"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFD06_09075"
     CDS             <1..387
                     /locus_tag="VFD06_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03722.1"
                     /translation="GAFTGTGLASFLGRKYGVTLMDASRIGSRTSDGARYALVVPSLS
                     EAYRGVAFRGRWPGPPDDPVESIEVDRESIAIRYRDGTVHVLAPTADGLVALYVSAAN
                     ASSLSKVDEADKALAALQRLGRGGGR"
     gene            384..1682
                     /locus_tag="VFD06_09080"
     CDS             384..1682
                     /locus_tag="VFD06_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03723.1"
                     /translation="MIRRLAPGRRLRLFVSAAAIGLLLAAAPSPAPADDSSEEMASLR
                     NDAISAYNDQNYPRAATLAQHYLERAGEESRTGREVAALQFILGHSRYEVHQKERGRY
                     EGDYKADVVQPLEESLRVLQDDAAFKNMLLGNAWHVLWSEGGRREAEAEGRGHWYLLK
                     SILLREAEARHRARTSPEYDQFARHVLFYLERCLEYIRTSASADLYRERIRVTAPLGF
                     GTGYDARFWQIHDLTYFDDGNMKAAALWQRGLDLMRDPKASPDEALASFTEATEITRR
                     RRDKAEVFRQMADFVSTLDAPKRRTQAAEFGRRAYELNPGDAEIRRQYGTALHVLSYA
                     AYATGKFQDAYARAQEAITFEWAGMETALFDLSRAAAEMGRETEALTHGERAYRMAKE
                     RLAGSALQPFAQNYVNVLRQFGQEGLATRVQIDNASLGVR"
     gene            1682..2791
                     /locus_tag="VFD06_09085"
     CDS             1682..2791
                     /locus_tag="VFD06_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03724.1"
                     /translation="MPRTAPGRSLVPRRRALVAAALLLGVLADGPQAAAPQETVAGAL
                     ELGGRWAALLRGSVAILDRRPDPATIQAALTDARRAVAEIDRLSANAAIFQAAPQDRQ
                     KLQDLAAAAHLNLALFETHGLEFERAREEIGRAKSLSDLVQNESFRTEWAAIPAGEPG
                     KALQTRYNLLTLGEFEAALGSIWSRARPVPFEFRGITAEELAQMRLSPATTPPPGSLD
                     ERLVLGATMALRAAVEKARPSFTLPLPPGVYRLQGRPSGDIDRTFVVPEATDVDTVLI
                     DRARFTLRLDPKSGPRPPRFFLNGFEVTDLMKMPYGVYRLKADPNDYPTAPQIVRFVL
                     GEGIPDKTRTSWTIYVPAGTPATLAFDRGPATRRR"
     gene            complement(2801..4123)
                     /gene="hemL"
                     /locus_tag="VFD06_09090"
     CDS             complement(2801..4123)
                     /gene="hemL"
                     /locus_tag="VFD06_09090"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZN03725.1"
                     /translation="MSDVPKKGTSETSARLFEKARQIIPGGVSSPVRAFRAVGGTPLF
                     FQRGRGAMLFDVDGKGYIDYVCSWGPLILGHADPGIVRKVIETVADGTSFGAPNPHEV
                     ALARLVVERVPGIEKVRLVNSGTEATLSALRLARAFTGRDLIVKMEGCYHGHVDALLV
                     KGGSGLVTLGIPGTPGVPARVVEQTLVVPYNDLDALERLIQERGPEIACVIVEPVAAN
                     MGVVPPRSGYLEGLRALTEKAGILLIFDEVITGFRLAPGGAQERYRITPDLTTLGKIL
                     GGGLPIGAYGGREEIMDRVAPEGDVYQAGTLSGNPVAACAGIAMLEALAAGDRYARLE
                     ETSARLEGGLKEAIAATGTDARVTRVGSLLTLFFGTGEPVDWASVAGADTKRYGLFFH
                     KMLEAGVYLPPSQYEAWFVSLVHDREVIDRTIEAAHQALKALQRAPLA"
BASE COUNT          606 a         1525 c         1456 g          569 t
ORIGIN      
        1 ggggcgttca ccggcaccgg cctcgcgtcg ttcctcggcc gcaagtacgg cgtgacgctg
       61 atggacgcca gccggatcgg gtcgcgcacc agcgacggcg cgcgctacgc gctcgtggtg
      121 ccgtccctct cggaggcgta ccgcggcgtc gcgtttcgcg gccggtggcc cggcccgccc
      181 gacgacccgg tggagagcat cgaggtggac cgcgaatcga tcgccatccg ctaccgcgac
      241 ggaacggtac acgtgctggc gccgaccgcc gacggcctcg tggcgctcta cgtctcggcg
      301 gcgaacgcgt cgagcctgtc caaggtcgac gaagcggaca aggcgctcgc ggcgctgcag
      361 cgtctcggcc ggggaggcgg gcgatgatcc gccgcctcgc tcccgggcgc cggctgcgcc
      421 tgttcgtctc ggccgcggcg atcggcctcc tcctcgctgc ggcgccgtcg ccggcgcccg
      481 cggacgactc gtcggaggag atggcctcgc tgcgcaacga cgccatcagc gcctacaacg
      541 accagaacta cccccgggcc gccaccctcg cccagcatta cctggagcgg gccggcgagg
      601 aatcccgcac cgggcgggag gtggcggcgc tgcagttcat cctcggccac tcgcgctacg
      661 aggtccacca gaaggaacgc gggcgctacg aaggggacta caaggccgac gtggtccagc
      721 cgctcgagga gagcctgcgg gtcctgcaag acgacgccgc gttcaagaac atgctgctcg
      781 gcaacgcctg gcacgtcctt tggtcggagg ggggacggcg cgaggccgag gcggaggggc
      841 gcggtcactg gtacctcctc aagtcgatcc tgctgcgcga ggcggaggcg cgccatcggg
      901 cgcgcaccag ccccgaatac gaccagttcg cgcgccacgt cctcttctac ctggagcgct
      961 gcctggagta catccgcacc tccgccagcg cggatctgta ccgcgagcgg atccgggtca
     1021 ccgcgcctct cgggttcggc accggatacg acgcgcgttt ctggcagatc cacgacctga
     1081 cctacttcga cgacgggaac atgaaggcgg cggcgctgtg gcagcgcggc ctggacctga
     1141 tgcgcgaccc caaggcgtcg ccggacgagg cgctcgcctc cttcaccgag gcgaccgaga
     1201 tcacccggcg ccgtcgcgac aaggccgagg tctttcgcca gatggccgac ttcgtctcga
     1261 cgctcgacgc gccgaagcgg cgcacgcagg cagcggagtt cggacgccgc gcctacgagc
     1321 tgaatcccgg agacgccgag attcggcggc agtacggcac ggcgctgcac gtcctgtcgt
     1381 acgccgccta tgccaccggc aagttccagg atgcgtacgc gcgcgcccag gaggcgatca
     1441 ccttcgaatg ggccgggatg gagacggcgc tgttcgacct gtcccgcgcc gccgccgaga
     1501 tgggccggga gaccgaagcc ctgacgcacg gcgagcgcgc ctaccgcatg gcgaaggaac
     1561 ggctcgccgg gtcggcgctg cagccgttcg cgcagaacta cgtcaacgtc ctgcggcagt
     1621 tcggccagga ggggctcgcc acgcgcgtgc agatcgacaa cgcctccctg ggggtgcgct
     1681 gatgccccga acggcgcccg gtcgatcgct cgtgccgcgc cggcgggctc tcgtcgccgc
     1741 ggcgctcctc ctcggcgttc tggcggacgg tccgcaggcc gccgccccgc aagagacggt
     1801 cgccggggcc ctcgagctgg gagggcgctg ggcggcgctt ttgcggggaa gcgtcgcgat
     1861 cctcgaccgc cgtcccgatc cggcgacgat ccaggcggcg ctgaccgacg cgcgtcgcgc
     1921 cgtggccgag atcgaccggc tgtcggcgaa cgccgccatc ttccaggccg ctccccagga
     1981 tcggcagaag ctccaggacc tggccgccgc ggcgcacctc aacctggccc tcttcgagac
     2041 gcacggcctg gagttcgagc gggcgcggga ggagatcggc cgggcgaaat cgctctccga
     2101 cctcgtgcag aacgaatcct tccgcaccga gtgggcggcg attccggcgg gagagccggg
     2161 gaaggcgctg cagacccgct acaacctcct gacgctcggg gagttcgagg cggcgctcgg
     2221 atcgatctgg tcgcgcgccc ggccggtgcc gttcgagttc cgcggcatca ccgcggagga
     2281 gctggcgcag atgcgcctct cccccgccac gacacccccg cccggaagcc tcgacgagcg
     2341 gctggtcctc ggcgccacga tggcgcttcg ggcggcggtc gagaaggcac gcccgtcgtt
     2401 caccctgccg ctcccgccgg gcgtctaccg cctgcagggc cggccgtcgg gggacatcga
     2461 tcgaacgttc gtggtgccgg aggcgaccga cgtggacacc gtgctcatcg accgggcccg
     2521 cttcacgctg cgcctcgatc cgaagtcggg tccgcgcccg ccgcgcttct tcctgaacgg
     2581 tttcgaggtg accgacctca tgaagatgcc gtacggcgtc taccgactca aggccgaccc
     2641 gaacgactac ccgacggcgc cgcagattgt ccgcttcgtc ctcggcgaag ggatcccgga
     2701 caagacccgc acctcgtgga cgatttacgt gccggccggc acccccgcca cgctggcgtt
     2761 cgaccgcggt cccgccacgc gccggcgctg atccggtcga tcaggcgagc ggggcgcgct
     2821 gcagggcctt cagcgcctgg tgcgccgctt cgatggtgcg atcgatgacc tcgcggtcgt
     2881 gcaccagcga cacgaaccag gcttcgtact gcgaaggcgg gaggtagacg cccgcctcca
     2941 gcatcttgtg gaagaagagg ccgtagcgct tcgtgtcggc gccggcgacc gacgcccagt
     3001 ccacgggctc gccggtgccg aagaacagcg tcaggagcga tccgacgcgc gtgacgcgcg
     3061 cgtcggtgcc agtcgccgcg atcgcctcct tcaggccgcc ttccaggcgc gccgacgtct
     3121 cctcgagccg ggcgtagcgg tcgccggctg cgagcgcctc cagcatggcg atgccggcgc
     3181 aggccgccac cgggttcccc gacagggtgc cggcctgata gacgtcccct tccggcgcga
     3241 cgcggtccat gatctcctcg cgcccgccgt aggcgccgat cggcaggccg cccccgagaa
     3301 tcttgccgag ggtcgtcaga tcgggggtga tccgatagcg ctcctgcgcg ccgcccggcg
     3361 ccagccggaa gccggtgatc acctcgtcga agatcaggag gatgccggcc ttctcggtca
     3421 gggcgcgcag cccttcgagg tagccggagc gcggcggcac gacccccatg ttcgccgcca
     3481 ccggctcgac gatgacgcag gcgatctccg ggccgcgctc ctggatcaac cgctccaggg
     3541 cgtcgagatc gttgtacggc acgaccagcg tctgctcgac gacgcgcgcc gggacgccgg
     3601 gcgtccccgg aatccccagc gtgacgagcc cgcttccccc cttgaccagg agcgcgtcga
     3661 cgtggccgtg gtagcagcct tccatcttca cgatgaggtc acgcccggtg aaggcgcgcg
     3721 ccaggcgcag ggccgaaagc gtcgcctcgg tccccgagtt caccaggcgc accttctcga
     3781 tcccggggac gcgctcgacc accaggcgcg ccagcgccac ctcgtgcggg ttcggcgcgc
     3841 cgaacgacgt cccgtcggca accgtttcaa tgaccttccg gacgatgccc gggtcggcgt
     3901 gccccaggat gagcgggccc cacgagcaga cgtagtcgat gtaccccttc ccgtcgacgt
     3961 cgaagagcat cgcgccgcgg ccgcgctgga agaagagcgg cgtgccgccg acggcgcgga
     4021 aggcccgcac gggagagctg acgccaccgg gaatgatctg gcgcgccttc tcgaacagcc
     4081 gcgcggaggt ctcgctcgtg cccttcttcg gaacgtcgct catggggcgc cgggtcgtct
     4141 cagccgcgca ggaggc
//
LOCUS       DASRIQ010000376         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3821441, whole genome shotgun sequence.
ACCESSION   DASRIQ010000376 DASRIQ010000000
VERSION     DASRIQ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3821441"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VFD06_09095"
     CDS             <1..1354
                     /locus_tag="VFD06_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HZN03726.1"
                     /translation="PMLWHAYGERIGAGAEALKQVEADILAGFAAARDVSERKKAVPA
                     LRTLPEYWRDYVGGAYDRRLEVPTAVAAERIEEIAARLTAVPDGFHLNPKVRKGLEQR
                     AEMAAGKRPVDFGMAEALAFGSLLLEGVPVRLSGQDSRRGTFNQRHSAWIDVETAAEH
                     LPLQHLAGRQARFEAHDSPLSEGSVLGFEYGYSRDTPEALVLWEAQFGDFTNGAQVII
                     DQFISAAEDKWNLWSGVVLLLPHGYEGQGPEHSSARLERFLQLCGEDNIQVCQPANAA
                     QYFHLLRRQALRRWRKPLVVMTPKSLLRADSASAPIAALSQGRFEPVIGDPRASECER
                     LLVCSGKVAHDLRAWREKKKCARVAIVTLEQLYPFPDEELRAALEPFQDRGEVVWVQE
                     EPANMGAETFVRRRIQQVVGSRHVSTVHRSTSASPATGSPDSHRLEQETLLSLACALT
                     "
     gene            complement(1379..2287)
                     /locus_tag="VFD06_09100"
     CDS             complement(1379..2287)
                     /locus_tag="VFD06_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZN03727.1"
                     /translation="MSIRPIKKMIQSKPTLEGAGVRLRRAFGFGNTSDFDPFLLLDDF
                     RNDRPEDYLAGFPWHPHRGIETITYVLAGTVEHGDSMGNKGVIAAGDVQWMTAGRGII
                     HQEMPKGDPDGRMHGFQLWANLPASLKMMAPRYQELKAGDVPVLKEDDGSEIRIVCGS
                     YRGKRGPVEGVAADPNYFDITIPAGLRKTLPIETTRHAFAYVFGGSGKVCNASGPLDV
                     PTEAAGWSDTHPPAAAENRSLVLFDRGDEVTVEAGEKGMRFLLVSGKPLNEPVAWYGP
                     IVMNTQAQLRQAFEELERGTFLKPGA"
     gene            complement(2360..3664)
                     /locus_tag="VFD06_09105"
     CDS             complement(2360..3664)
                     /locus_tag="VFD06_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN03728.1"
                     /translation="MPDTALLLLQLVVVLATARLAGRAAAFLGQPRVIGEMVAGLALG
                     PSLFGWLRPDWMHALFPADRMGSLSTLSNLGVILFMFVVGLHLDGALLGQRAKAAVAV
                     SWASIVVPFALGAALAPWLHPGFAGTGVALLPFTLFLGAAMSVTAFPVLARILNERRL
                     MNTPAGSIAIAAAAVDDVSAWCLLAVVVAVARGAAAGGRFLLLFLGSAAYVLFMLFVV
                     RPALRRWQARRVRARAAVSAAGVSAAQEERAAAGAEAIGAAVLLALGSALVTEWLGVH
                     ALFGAFLAGAIVPREGGLARDLAGRLEGMVTTVLLPLFFAFTGLKTEIGLVNGALWGV
                     AAVILLAAMAGKLGGAAGAARLAGMTWRDALAVGVLMNTRGLMELVILTIGLEIGVLS
                     PTLFAMMVFMALVTTVMTAPLLGVILRRRAGAEATPAMAMRP"
     gene            complement(3680..>3814)
                     /locus_tag="VFD06_09110"
     CDS             complement(3680..>3814)
                     /locus_tag="VFD06_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HZN03729.1"
                     /translation="PSHRREYIEWITEAKREETRRKRMETSLQWMVEGKARNWKYQAR
                     "
BASE COUNT          575 a         1375 c         1357 g          507 t
ORIGIN      
        1 cccgatgctg tggcacgcgt acggcgaacg gatcggcgcc ggcgccgagg cgctgaaaca
       61 ggtcgaggcg gacatcctcg ccggcttcgc cgcggcgcgc gacgtgtcgg agcggaagaa
      121 ggcggtgccg gcgctgcgga ccctcccgga gtactggcgc gactacgtcg gcggggccta
      181 cgaccgccgc ctcgaggtgc cgaccgccgt tgcggcggag cggatcgagg agatcgccgc
      241 gcgcctcacc gccgttccgg acggcttcca cctcaacccc aaggtgcgca aggggctgga
      301 gcagcgcgcc gagatggcgg ccggcaagcg gccggtcgat ttcgggatgg cggaggcgct
      361 ggcgttcgga tcgctcctgc tcgaaggggt cccggtgcgc ctctccggcc aggacagccg
      421 ccgtggcacc ttcaaccagc ggcactccgc ctggatcgac gtggagacgg cggccgagca
      481 cctgccgctg cagcacctcg cgggtcggca ggcccgcttc gaggcacacg attcgccgct
      541 ctcggagggg tcggtcctcg ggttcgagta cggctattcg cgcgacacgc ccgaggcgct
      601 ggtgctgtgg gaggcccagt tcggcgactt caccaacggc gcgcaggtga tcatcgacca
      661 gttcatcagc gccgccgagg acaagtggaa cctctggtcc ggcgtagtcc tgctgctgcc
      721 gcacggctat gaagggcagg ggccggagca ctcgagcgcg cgtctcgagc gcttcctgca
      781 gctctgcggc gaagacaaca tccaggtctg ccagccggcg aacgcggcgc aatacttcca
      841 cctcctgcgg cgccaggcgc tgcggcgctg gcgcaagccg ctcgtcgtga tgaccccgaa
      901 gagcctgctg cgggccgact cggcctcagc gccgatcgcg gcgctgtcgc aggggcgctt
      961 cgagccggtg atcggcgatc cgcgggcgtc ggagtgcgag cgcctgctcg tctgctccgg
     1021 caaggtggct cacgacctgc gcgcctggcg cgagaagaag aaatgcgccc gggtggcgat
     1081 cgtcaccctc gagcagctct acccgttccc cgacgaggag ttgcgcgcgg cgctcgagcc
     1141 gttccaggat cgcggagagg tcgtctgggt gcaggaagag ccggcgaaca tgggggccga
     1201 gacgttcgtg cggcggcgta tccagcaggt cgtcggcagc cggcacgtct cgacggtgca
     1261 ccgctcgacg agcgccagcc cggcgaccgg ttcgcccgac tcgcaccggc tcgagcagga
     1321 aacgctcttg agcctcgcct gcgcgctgac ctgatgcggc gcctcccggg cgccccggtc
     1381 aggcgcccgg tttcaggaag gtcccgcgct ccagctcctc gaacgcctgc cgcagctgcg
     1441 cctgggtgtt catcacgatc ggcccgtacc aggcgaccgg ctcgttgagc ggcttgccgg
     1501 agacgagcag gaagcgcatc cccttctcgc ccgcctcgac ggtcacctcg tcgccgcggt
     1561 cgaagaggac gagcgagcgg ttctccgccg ccgccggcgg gtgcgtgtcg gaccagccgg
     1621 ccgcctcggt cggcacgtcg agcggaccgg acgcgttgca caccttgccg ctgccgccga
     1681 agacgtacgc gaaggcgtgg cgcgtcgtct cgatcggcag cgtcttccgc aggcctgccg
     1741 ggatcgtaat gtcgaagtag ttcggatcgg ccgccactcc ctcgacgggg ccgcgctttc
     1801 cgcggtacga gccgcagacg atgcggatct cggagccgtc gtcctccttc aggaccggga
     1861 cgtctcccgc cttcagctcc tggtagcgcg gcgccatcat cttgagcgac gccggcaggt
     1921 tcgcccagag ctggaagccg tgcatgcggc cgtccgggtc tcccttcggc atctcctggt
     1981 ggatgatgcc gcgccccgcg gtcatccact gcacgtcgcc cgccgcgatc acccccttgt
     2041 tccccatgct gtcgccgtgc tcgaccgttc ccgccaggac gtaggtgatc gtctcgatgc
     2101 cgcgatgcgg gtgccagggg aagccggcga ggtagtcctc cggccggtcg ttgcggaaat
     2161 cgtcgagcag caggaagggg tcgaagtcgg aggtgttgcc gaagccgaac gcccgccgca
     2221 ggcggacacc ggccccttcg agggtgggct tggactggat catcttcttg atcggacgaa
     2281 tcgacatcgg agcctcccgg ggatcgccgc catcatagac ccgccagcgc ccggcgacct
     2341 cctccggctc gggcgcccgc tacggtcgca tggccatggc cggggtcgcc tcggcccccg
     2401 cccggcgccg caggatcact cccaaaagcg gcgcagtcat caccgtggtg acgagcgcca
     2461 tgaagaccat catggcgaac agcgtcgggc tgaggacacc gatctccagg ccgatcgtca
     2521 ggatcaccag ctccatgagg ccgcgcgtgt tcatcaggac gccgaccgcc agcgcatccc
     2581 gccaggtcat cccggccagc cgggcggcgc ccgcggcccc tcccagcttg ccggccatcg
     2641 cggcgagcag gatcacggcc gccacgcccc agagcgcgcc attgacgagt ccgatctccg
     2701 tcttgagacc ggtgaacgcg aagaacagcg gcaggagcac cgtcgtcacc atcccctcga
     2761 gacggccggc gaggtcgcgc gccagtcccc cctcgcgcgg gacgatggcg ccggccagga
     2821 aggcgccgaa cagcgcgtgc acccccagcc actcggtgac gagcgcggag ccgagggcga
     2881 ggaggacggc ggcgccgatc gcctccgctc ccgcggcggc ccgttcctcc tgcgccgcgg
     2941 agactcccgc cgcggagacg gcggcgcgcg cccgcacgcg ccgcgcttgc cagcggcgca
     3001 gggccggccg caccacgaac agcatgaaca ggacgtaggc ggcggagccg aggaacagca
     3061 ggaggaaccg cccgccggcg gcggcgccgc gggcgaccgc caccacgacc gccaggaggc
     3121 accaggcgct gacgtcgtcc accgccgccg cggcgatggc gatcgagccg gccggggtgt
     3181 tcatgagccg gcgctcgttg aggatgcgcg ccagcaccgg gaacgccgtc acgctcatcg
     3241 ccgcgccgag gaacagcgtg aagggcagaa gcgcgacgcc ggttccggcg aagccgggat
     3301 ggagccacgg cgccagcgcg gcgccgagtg cgaacggcac gacgatgctc gcccagctga
     3361 cggcgaccgc cgccttcgcc cgctgcccga gcagcgcccc gtcgagatgc agcccgacga
     3421 cgaacatgaa caggatgacg ccgaggttgc tgagggtgga gagggagccc atccggtcgg
     3481 ccgggaagag agcgtgcatc cagtccggac ggagccagcc gaacagggac ggaccgagcg
     3541 ccagcccggc caccatctcg ccgatgacgc ggggctgccc caggaaggcg gccgcccggc
     3601 cggcgaggcg ggcggtggcg agcaccacga cgagctggag gagcagcagg gcggtgtcag
     3661 gcatgtcgcc gcgcggaatc tagcgggcct ggtacttcca gttccgcgcc ttcccctcga
     3721 ccatccactg cagcgaggtc tccatgcgct tccgccgggt ctcctcgcgc ttcgcctcgg
     3781 tgatccattc gatgtactcg cgcctgtggc tcgg
//
LOCUS       DASRIQ010000377         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1463100, whole genome shotgun sequence.
ACCESSION   DASRIQ010000377 DASRIQ010000000
VERSION     DASRIQ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463100"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="VFD06_09115"
     CDS             complement(<1..1864)
                     /locus_tag="VFD06_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN03730.1"
                     /translation="MRLAMVPRRRLFSGILTAVVLTGLALAGLARVPPGSMAARSWRG
                     GGSPELLGPGWAVRLPWLQTLELFPEGEIRTSGRCDASSREGTRVGLPFEVRLKPGSQ
                     GLLELAREAPGEAAGALGRRASERLADLAAGWGTYDLASGQAREPLEAGLREHLRAGR
                     PDASVEVRLGEPELAPEVRASFARETLFSSRAETGVRLLLIGLDGADWDVIDPMIARG
                     ELPHLASLRREGAWGRLRSNVPTLSPLLWTTVATGKSPDRHGINDFLVVDPKSGRRVP
                     INATFRKVRAFWNILTEAGLPVDVVAWWASWPAEPIRGHLISDRVAYSTFDISGAGAA
                     GGAVFPPDYLATVDRLRVLDSDITSQQVSRFLRLAPGEFQAMMQAGRDARPAGARPVP
                     EATESVQVFTRVLAATESYRRIALDLLRNGGAAPRLLAVYFQGIDEVNHRFAHCASPR
                     APLCPEADYRRFKDAVASFYRYQDGIVGELLAAAPGATTLLLSDHGFASGEARPTDVK
                     PFIEGKPGMWHDIVGIFAARGPAIRSGELPTVTLYDIAPTVLYLLGLPVAQDMPGRVL
                     EQAIAPEFLAAHPIARVPSYEGLGPEPSETASGSGAPDAAGTAADEEMIAQLKSL"
     gene            complement(1877..2377)
                     /gene="ruvC"
                     /locus_tag="VFD06_09120"
     CDS             complement(1877..2377)
                     /gene="ruvC"
                     /locus_tag="VFD06_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004869369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZN03731.1"
                     /translation="MLILGLDPGSQRTGYGLIESRGGRLRAIAFGVIRPPRDTSFLER
                     LPHLARQLEVLLERARPDAAAAEDIFLTRNSRVALKLGHVRGAALLPVLAAGIPVHEY
                     AARLVKKTVTGFGGAEKEQVCRMVRLLLGLRDAIPTLDASDALAVAICHAHAGHAFAR
                     PPAARA"
     gene            complement(2384..>2835)
                     /locus_tag="VFD06_09125"
     CDS             complement(2384..>2835)
                     /locus_tag="VFD06_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN03732.1"
                     /translation="TDNKNRTLPEIRHLFSKFGGNLGESNCVGWMFEKKGYLVVAKNA
                     ASEDTLMELVIEAGGDDVRDDGDNWEIFTPTDRLEQVREAIAGRGITIGTAELSMVPK
                     NTVKVEGKKAQQVLSMMEGLEEHDDVQNVWANFDIDDAEISEERAAS"
BASE COUNT          426 a         1011 c         1010 g          388 t
ORIGIN      
        1 cgagcgactt cagctgcgcg atcatctcct cgtccgccgc cgtcccggcg gcatccgggg
       61 cgccgctgcc cgaagcggtc tccgacggct ccgggccgag cccctcgtac gacgggacgc
      121 gcgcgatcgg gtgggccgcc aggaactcgg gcgcgatcgc ctgttcgagc acgcgtccag
      181 gcatgtcctg cgccaccggc aggccgagca gatagagaac cgtcggcgcg atgtcgtaca
      241 gcgtgacggt cggcagctcc cccgagcgga tcgccggccc gcgggcggcg aagatgccga
      301 cgatgtcgtg ccacatgccg ggcttccctt cgatgaacgg cttcacgtcg gtcgggcggg
      361 cctcgccgct ggcaaagcca tggtccgaga ggagcagtgt cgtcgcgccg ggcgcggcgg
      421 cgagcagctc gccgacaatc ccgtcctggt agcggtagaa ggaggcgacg gcatccttga
      481 agcgacgata gtcggcctcg gggcagagcg gggcgcgcgg cgaagcgcag tgggcgaagc
      541 ggtggttcac ctcgtcgatc ccctggaagt acaccgccag cagtcgcggg gccgctccgc
      601 cgttccgcag cagatcgagg gcgatccggc gataggactc cgtggcggcc aggacgcgcg
      661 tgaacacctg caccgattcg gtcgcctccg gaaccgggcg cgcgccggcg ggccgggcgt
      721 cacgcccggc ttgcatcatg gcctggaact cgccgggcgc caggcgcagg aagcgcgaca
      781 cctgctgcga agtgatgtcg gagtcgagca cgcggagacg atcgacggtc gcgaggtagt
      841 ccggcgggaa gacggcgccg ccggcggccc cggccccgga gatgtcgaag gtcgagtagg
      901 cgacgcggtc ggagatcagg tgcccgcgga tcggctccgc cggccagctg gcccaccagg
      961 ccaccacgtc gaccggaagc ccggcctccg tcaggatgtt ccagaaggcg cggacctttc
     1021 ggaaggtcgc gttgatcggc acccggcggc cgcttttcgg atcgacgacg aggaagtcgt
     1081 tgatgccgtg gcgatcgggg gatttgcccg tggccactgt cgtccaaagg agcggcgaca
     1141 gcgtcggaac gttgctgcgc agcctccccc acgcgccttc ccggcgcagc gacgcaaggt
     1201 gcggcagctc cccgcgggcg atcatcggat cgatcacgtc ccagtcggcg ccgtcgaggc
     1261 cgatcaggag gagccgcaca ccggtctccg cgcgggacga gaacagggtt tcccgggcga
     1321 aggaggcgcg cacctcgggg gccagctccg gctctccgag gcgcacctcg accgacgcat
     1381 cgggacggcc ggcgcgcagg tgctcgcgca gtcccgcctc gagcggttcg cgcgcctggc
     1441 cggaggccag atcgtaggtg ccccagccgg ccgccaggtc ggcgaggcgc tccgacgcgc
     1501 gccgccccag cgccccggcc gcctcgccgg gcgcctcgcg cgcgagctcc agaagcccct
     1561 gcgatccggg cttcaagcgc acttcgaacg ggagccccac acgcgtcccc tcgcgtgacg
     1621 acgcgtcgca gcgccccgat gtccggatct ccccctcggg gaacagctcg agcgtctgca
     1681 gccacgggag gcgcaccgcc cagccggggc cgagcagctc cgggctccct cccccgcgcc
     1741 aggaacgcgc cgccatcgat cccgggggaa cgcgggcgag gcccgccagc gccaggccgg
     1801 tgaggaccac cgccgtcagg atccctgaaa aaagacggcg gcggggcacc atcgcgagcc
     1861 tcatgccggg accacctcag gcccgcgcgg cgggggggcg cgcgaaggcg tgaccggcat
     1921 gcgcgtggca gatggccacc gcgagggcgt ccgaggcgtc cagggtcggg atggcgtccc
     1981 gtagtccgag gagcagccgc accatgcggc acacctgctc cttttcggct ccgccgaacc
     2041 cggtgacggt ctttttcacc aggcgcgcgg cgtactcgtg gaccgggatg ccggcggcga
     2101 gcaccggcag gagcgccgcg ccgcgcacgt gcccgagctt gagcgcgaca cggctgttgc
     2161 gggtgaggaa gatgtcctcc gccgcagcgg cgtcgggacg ggcgcgctcg aggaggacct
     2221 ccagctgacg cgcgaggtgc ggcaggcgct cgaggaagga cgtgtcgcgc gggggccgga
     2281 tgaccccgaa ggcgatggcc ctcaaccgcc cgccgcgact ctcaatcagc ccgtaaccgg
     2341 tccgttgcga accgggatcg aggccgagga tgagcatccg gggtcaggac gccgcccgct
     2401 cctcgctgat ctcggcgtcg tcgatatcga agttcgccca gacgttctgg acgtcgtcgt
     2461 gctcttcgag gccttccatc atggagagga cctgctgggc cttcttcccc tccaccttga
     2521 cggtgttctt cggaaccatc gacagctctg cggtcccgat ggtgatccca cggcccgcga
     2581 tcgcctcgcg cacctgctcg agccgatcgg tgggagtgaa gatctcccag ttgtcgccgt
     2641 cgtcgcgcac atcgtccccg cccgcctcga tgaccagctc catcagggtg tcctcgctcg
     2701 cggcgttctt cgcgaccacg agatacccct tcttctcgaa catccagccg acgcagttcg
     2761 attccccgag attgccgccg aacttcgaga agagatgccg aatttccggc agcgtgcggt
     2821 tcttgttgtc ggtca
//
LOCUS       DASRIQ010000378         6016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_399351, whole genome shotgun sequence.
ACCESSION   DASRIQ010000378 DASRIQ010000000
VERSION     DASRIQ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399351"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFD06_09130"
     CDS             <1..909
                     /locus_tag="VFD06_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03733.1"
                     /translation="GRAHAVPRFRCRVCRRGFSRQTFRADYRQKKPHLNAGCLRLFVS
                     CVGQRQAARVLQVARRTIERRFRWLARHAEGFHRNRLHGAALSGPFQLDELESFEANR
                     YQPVTVPVLIDRKTFFMVAAAVGSLRRKGRMSLRQRHERARHEALHGGRPSHSAQAVG
                     QVLSRLRPMLHPSRPFVLDSDQKPLYGRLGRRLFGARFRWSQHSASARRDRSNPLFPI
                     NHTNARLRHFLSRLRRRSWCVSKTMRDLKRHLEIAAMWVNYSRGITNRTSVTPAQALG
                     LLPRPLRPEELLAWRQDWGPRSPDIL"
     gene            complement(963..1685)
                     /locus_tag="VFD06_09135"
     CDS             complement(963..1685)
                     /locus_tag="VFD06_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN03734.1"
                     /translation="MPAPVGGDAPLVLLADANRPVRSLLRPMLAELGCRVETADDGTA
                     AFRAVVARHPAAIVASLHLGGLSGVAICEGVKGSPHLREIKVALVGSDLSADLFNRDT
                     ALAYGADLFLEEGMSEDTLRASLAAILPHAPDPGRPSEEAALDVALDDLDAEEGGPRA
                     GGPAAEQIRRLARLMLSDLKLYNPDRFTQAVQSGQVLEIFKSELARGRDLVDQRFPGL
                     PARQDLLLNALRDGIARETGSR"
     gene            complement(1874..3391)
                     /locus_tag="VFD06_09140"
     CDS             complement(1874..3391)
                     /locus_tag="VFD06_09140"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZN03735.1"
                     /translation="MSVVGNLEDLSFPDILQVVHLSRQSGTLILSGKEGERRVRFRDG
                     LVCDASLGEGKPRLDELLIARGLVAPAALEPARARAASSGESLAASLVALGALSQETL
                     DRVVREELRDLVRSFVLLQEGEFRFELDEGAVPPEEAPAPGLGPDAILGGLPQAAPPR
                     WPEATAGGVPRRVLLASERSFLTLALREELERDGFEVVPCESAARGESLGRDLVARGE
                     PFFLVCDLILPDATRTGWGGGMDLVRALRAAAPGLIVVMIGEVRHPSAAAAARAAGAT
                     GYLTLPDLAEVPFEEVGVRVRDFCVSLGSALCFSDQLAQIDWSAGGRTVRVADPLQLL
                     RGLIGELQAEPPGDVSLLVLRLASEYFERAALFAVADGRAICRGAFGKPVDERMRGAT
                     FPLEAGSILRLAVDRGEATVGPLPENAGNGALRARLGPPEPKNAAVLPVHGGREVFGV
                     LYGDNAVSDEPFDDLRPLEIFLSQAGLTLQNALLKRRLETLESGTASLTGTERAA"
     gene            complement(3484..3969)
                     /gene="nusB"
                     /locus_tag="VFD06_09145"
     CDS             complement(3484..3969)
                     /gene="nusB"
                     /locus_tag="VFD06_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZN03736.1"
                     /translation="MPSRHQAREYALQMLYQAEVGSMSMPDVVAGFFRPDEEVPEEVR
                     RFASRLALGTSQALEEVDALLREGIEHWRLERLGTVDRAVLRLAVFEFLHEPATPRIV
                     IIDEAIEVAKRYGGEESGQFVNGILDALRKRLEGAGAPPAGDDENVIPPPAGGGPPSK
                     E"
     gene            complement(3973..4491)
                     /gene="ribH"
                     /locus_tag="VFD06_09150"
     CDS             complement(3973..4491)
                     /gene="ribH"
                     /locus_tag="VFD06_09150"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZN03737.1"
                     /translation="MTLPRRPHLHEIRGTFDGTGLRIGIVAARFNDFIVERLLQAALD
                     ALRRHGVRDADVTVARVPGALEIPVAARALAASGSADAVVTLGCVIRGDTVHYDHVCS
                     EVSRGVSLAALDTGVPVTFGVVAAESLEQAIERAGAKAGNRGWDAAVAAIEMATLLRR
                     LPGAADASAGRS"
     gene            complement(4541..5302)
                     /locus_tag="VFD06_09155"
     CDS             complement(4541..5302)
                     /locus_tag="VFD06_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03738.1"
                     /translation="MTSGASAPRRPLLVLLPAMLAPAAALAAVALAGPWPSLGRAALP
                     ALATAAVYPVFAFFVARGARRSAVLSALLWAASLSAAIIALAARDPEGTGRAVLMGPS
                     YRDEMFQYVASGAGRETDPGRFVPQHLLHAGLFSVVTLLSGGLLGLAMGSALVGYMSF
                     YVGALAGGPHPATAALLGWPPWAIVRVVAFVLLGAVLARPLLVRLRGAPAAPLFEPDD
                     RRLLLAALVLLVVDIVLKTAFAATWSALLRPCLPT"
     gene            complement(5299..>6016)
                     /locus_tag="VFD06_09160"
     CDS             complement(5299..>6016)
                     /locus_tag="VFD06_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZN03739.1"
                     /translation="SGLEVADGQTFRWSGEYSEDLNERTTHATHLNVFQSFRPRMRKQ
                     DKGAPFLFLGNIDPDLQSQVLAEVKPEFVACDTMNFWIEGKSDSLKRLLGSVHGLVIN
                     DSEARLLAGELNTVAAARRLLDLGLKFLVIKRGEFGAALFHGGHYFAVPAYPVERPVD
                     PTGAGDSFAGGFMGHLAREKKVGGPAMRQAMIYGTVMASFCVEDFSLRRLAAVSEKGI
                     EARAAELREFLNVDGPSRKR"
BASE COUNT          894 a         2175 c         2179 g          768 t
ORIGIN      
        1 ggacgggcgc atgccgtgcc gcgcttccgc tgccgcgtgt gccggcgcgg attctcgcgg
       61 cagacgtttc gcgccgacta tcggcagaag aagccgcacc tgaatgccgg ttgtctccgg
      121 ctgttcgtct cctgcgtcgg ccagcggcag gcggcgcggg tcctgcaggt ggcccggcgc
      181 accatcgaac gccgctttcg ctggctcgcc cgacatgccg aagggtttca tcgcaatcgt
      241 cttcacggcg cagcgctctc cggcccattc cagctcgatg agctggagtc gttcgaagcg
      301 aaccgctacc agccggtaac cgtgcccgta ctcatcgacc gcaagacgtt cttcatggtg
      361 gcggccgccg tcggctccct ccgccgcaaa ggtcgtatga gtctgcgcca gcggcacgag
      421 agggcaaggc acgaggcgct gcatggcggc cgtccttctc attcggcgca ggcggtcggg
      481 caggtcctct ctcgcctccg gccgatgctc caccctagcc gccccttcgt gctggactcc
      541 gaccagaagc cgctgtacgg acggctcggc cgccgcctgt tcggagcgcg ctttcgctgg
      601 agtcaacact ccgcttcggc ccggcgcgac cgctcaaacc ctctgttccc gatcaatcac
      661 acgaacgcgc ggctgcgaca cttcctgtcg cgcctgcgtc gacgcagctg gtgcgtctcg
      721 aagacgatgc gggatctgaa gcgtcatctg gagatcgcgg cgatgtgggt caactactcg
      781 cgcggcatca ccaatcgcac ttcggtgacg cccgcgcagg ctctcggcct gctccctcga
      841 cccctgcgcc ctgaggaact gctcgcctgg cgccaggatt ggggacctcg gagcccggac
      901 atcctctgac gggtgtcccc acgcttgtcg ggcattttct gactcagtaa ggggcggcgg
      961 actcagcggg agccggtctc gcgggcgatg ccgtcgcgca gcgcgttcag aaggaggtcc
     1021 tggcgggccg gcagccccgg gaatcgctga tcgacgaggt cgcgtccgcg cgccagctcc
     1081 gacttgaaga tctccagcac ctggccgctc tgcaccgcct gggtgaagcg atcggggttg
     1141 tagagcttga gatcggagag catcagccgc gcgaggcggc ggatctgctc cgcggcgggc
     1201 ccgccggcgc ggggtccccc ctcctccgcg tcgagatcgt cgagcgcgac gtcgagcgcg
     1261 gcctcctcgg acggacgtcc cggatccggc gcgtggggga ggatcgccgc gagcgacgcc
     1321 cgcagcgtgt cctcgctcat cccctcctcg aggaacagat cggcgccgta cgccagggcg
     1381 gtgtcgcggt tgaacaggtc ggcggagaga tcggagccga ccagcgcgac cttgatctcc
     1441 ctgaggtgcg gagacccctt caccccttcg cagatcgcca cccccgacag gccgccgagg
     1501 tgcaggctcg ccacgatcgc cgccggatgg cgcgccacca ccgcccggaa tgcggcggtg
     1561 ccgtcgtccg ccgtctcgac gcggcatccg agctccgcga gcatcggccg cagcaggctc
     1621 cggaccggac ggttggcgtc cgcgagcaga acgagcggcg cgtcgccgcc gaccggcgcc
     1681 ggcacgcgct ccggtcggac tctcggcgcg ggcggcggtc cgtcggggag cggcggggcc
     1741 ggtcgggagg ccgggccctc gggggccggg actccaaaaa gatggccgca gcgcgcgcag
     1801 cgcagcttcg aacccgggcg gacccgccca tcgtcgagtc ggaactgccg gcggcaggcg
     1861 gggcagccga cgatcacgcc gcccgctccg tcccggtgag agacgcggtc cccgactcga
     1921 gcgtctcgag gcggcgcttg agcagagcgt tctgcagcgt caggccggcc tgggacagga
     1981 agatctcgag cggccgcagg tcgtcgaacg gctcgtcgct gaccgcgttg tcgccataga
     2041 gcaccccgaa cacctcgcgt ccgccgtgca ccggcaggac cgccgcgttc ttcggctcgg
     2101 gggggccaag ccgcgcccgg agcgctccgt tgccggcgtt ctcgggaagt ggccccacgg
     2161 tcgcctcccc ccgatcgacg gcgagcctca ggatcgaacc ggcctcgagc gggaacgtcg
     2221 cgccgcgcat ccgctcgtcc accggcttgc cgaaggcgcc gcggcagatc gcgcgtccgt
     2281 cggcgacggc gaagagcgcc gcgcgctcga agtactcgga cgcgaggcgc agcaccagga
     2341 gcgacacgtc ccccggcggc tccgcctgca gctccccgat gagtccgcgc aggagctgca
     2401 gaggatcggc gacccggacc gtccggccgc cggcgctcca gtcgatctgc gccagctggt
     2461 cggagaagca cagcgccgaa cccagcgaca cgcagaagtc gcgcacccgc accccgacct
     2521 cctcgaacgg cacctccgcc agatcgggca gggtcaggta gccggtcgcg ccggcggcgc
     2581 gggccgcggc ggcggcgctc ggatgccgga cctccccgat catcaccacg atgaggccgg
     2641 gcgcggccgc gcgcagcgcg cgcacgagat ccatcccgcc cccccagccg gtgcgcgtcg
     2701 cgtccggcag gatcaggtcg cagacgagga agaacggctc gccgcgcgcg acgaggtcgc
     2761 gtcccaggct ctcgccgcgc gccgccgact cgcagggcac gacctcgaac ccgtcccgct
     2821 ccagctcttc gcgcagcgcc agggtcagga acgaccgctc gctcgccagg agcacgcgcc
     2881 gcggcactcc gccggcggtg gcctccggcc agcgcggcgg cgccgcctgc ggcagcccgc
     2941 cgaggatcgc gtccggaccg agcccggggg ccggggcctc ctccgggggg acggccccct
     3001 cgtccagctc gaagcggaac tccccttcct ggaggagcac gaaggagcgg accagatccc
     3061 ggagctcctc gcggacgacc cgatcgaggg tctcctgcga cagcgcccca agcgcgacca
     3121 gcgacgcggc cagcgattcg ccgctcgagg ccgcgcgggc ccgcgccggc tcgagcgctg
     3181 ccggagcgac gagcccgcgc gcgatcagga gctcgtcgag ccgcggcttt ccttctccca
     3241 ggctggcgtc gcacacgaga ccgtcgcgga agcggacccg ccgctcccct tccttgccgg
     3301 agaggatgag cgtgccggac tgccggctca gatggacgac ctgcaggatg tcgggaaagg
     3361 agagatcttc cagattgccg acgacgctca agggtccctc atcgggtccg cgtccggaca
     3421 cgggtcgaga cccgccggcg gtcgtgtccg gccgcccggc gggcgggtga tcaagaaacc
     3481 aatctattct tttgagggag ggccgccacc ggcaggagga gggatcacgt tttcgtcatc
     3541 gcctgcgggc ggggcgccag ccccctcgag gcgcttccgc agggcgtcga ggatgccgtt
     3601 gacgaactgc cccgactcct cgcccccgta gcgcttggcg acctcgatcg cctcgtcgat
     3661 gatcacgatg cgcggggtgg ccggctcgtg caggaactcg aagaccgcca ggcgcaggac
     3721 cgcccggtcc accgtcccga gccgctccag ccgccagtgc tcgatcccct cgcgcagcag
     3781 ggcgtccacc tcctcgagcg cctgggacgt gccgagggcc agccgcgacg cgaagcggcg
     3841 gacctcctcg gggacctcct cgtcggggcg gaagaacccg gcgacgacgt cgggcatcga
     3901 catgcttccg acctccgcct ggtagagcat ctgcagcgcg tactcgcgcg cctggtgccg
     3961 ggacggcatc gctcagctcc tccccgccga ggcgtccgcc gcccccggca gccggcgcag
     4021 aagcgtcgcc atctcgatcg ccgccaccgc ggcgtcccag ccgcggttgc cggccttggc
     4081 gccggcccgc tcgatcgcct gctcgaggct ctcggcggcg acgacgccga acgtcaccgg
     4141 cacgccggtg tcgagcgccg cgagcgacac cccgcgcgac acctcgctgc acacgtggtc
     4201 gtagtggacc gtgtccccgc ggatgacgca gccgagcgtg acgaccgcgt cggccgagcc
     4261 ggaggcggcg agggcccgcg ccgcgaccgg gatctcgagc gctcccggga cccgtgcgac
     4321 ggtgacgtcg gcgtcgcgca cgccgtggcg ccgcagcgcg tcgagcgccg cctgcagcag
     4381 tcgctccacg atgaagtcgt tgaagcgcgc tgcgacgatg ccgatgcgca ggccggtgcc
     4441 gtcgaatgtg ccgcggatct cgtgcaagtg aggtctccga gggagcgtca tcgtagtgag
     4501 gggttccggg ggtgtcaagg atcgcggcgg gcgcgggcga tcaggtgggg aggcaggggc
     4561 ggaggagggc ggaccaggtg gcggcgaagg cggtcttgag gacgatgtcc acgaccagga
     4621 ggaccagggc ggcgaggagg aggcggcgat cgtcgggttc gaagagcggc gccgccgggg
     4681 ccccacggag gcgcacgagg agcgggcggg cgaggacggc gccgaggagg acgaaggcga
     4741 cgacgcggac gatcgcccag gggggccagc cgagaagcgc ggcggtggcg gggtgaggtc
     4801 cgccggcgag cgccccgacg tagaacgaca tgtagccgac gagcgcagac cccatcgcga
     4861 gaccgaggag gcccccgctg agaagggtca cgacggagaa gaggccggcg tggagcagat
     4921 gctgagggac gaagcggccc gggtcggtct cgcggccggc gccgctcgcg acgtactgga
     4981 acatctcgtc gcggtatgac ggccccatga ggacagcgcg gccggtccct tccgggtcgc
     5041 gcgcggcgag agcgatgatc gccgccgaga gcgacgccgc ccacaggagc gccgacagga
     5101 ccgcggaccg tcgcgcaccg cgcgcgacga agaaggcgaa gaccggatac acggccgcgg
     5161 tggcgagggc cggcagcgcg gcgcggccga gcgacggcca ggggcccgcc agtgcgacgg
     5221 cggcgagcgc cgcggcgggg gcgagcatcg ccggaaggag gacgagcaga ggccggcgcg
     5281 gggcggacgc cccggaggtc atcgcttgcg ggacgggccg tcgacgttga ggaactcgcg
     5341 gagctcggcg gcgcgcgcct cgatcccctt ctcgctcacc gccgcgaggc ggcgcaggct
     5401 gaagtcctcg acgcagaacg aggccatcac tgtgccgtag atcatcgcct ggcgcatcgc
     5461 cggaccaccg acctttttct cacgcgccag atgccccatg aaaccgccgg cgaacgagtc
     5521 ccccgccccg gtcggatcga ccggccgctc gaccggatac gccggcaccg cgaagtagtg
     5581 gccgccgtgg aagagggcgg cgccgaactc gccgcgcttg atcaccagga acttgagccc
     5641 gaggtcgagg aggcgccggg ccgccgccac cgtgttgagc tccccggcga gaagccgcgc
     5701 ctcggagtcg ttgatcacca gtccgtggac ggatccgagc aggcgcttca ggctgtcgct
     5761 cttcccctcg atccagaagt tcatcgtgtc gcaggcgacg aactccggct tcacctccgc
     5821 gagcacctgg ctctggaggt cgggatcgat gttccccagg aagaggaaag gcgccccctt
     5881 gtcctgcttc cgcatgcggg gacgaaacga ctggaagacg ttcaggtggg tggcgtgcgt
     5941 cgtccgctcg ttgagatcct cggagtactc gccgctccag cggaaggtct ggccgtccgc
     6001 gacctcgagt ccggac
//
LOCUS       DASRIQ010000379         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_685512, whole genome shotgun sequence.
ACCESSION   DASRIQ010000379 DASRIQ010000000
VERSION     DASRIQ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685512"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFD06_09165"
     CDS             complement(<1..430)
                     /locus_tag="VFD06_09165"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN03740.1"
                     /translation="MSQPRPTYHSGELEVQRRAGVRNRADDVGRIIAGAIPMDLGPLL
                     AEFRLAVAASIDPRGRVWASLLTGPAGFLDIVDETRLHIGARVAPGDPLRTNLAGRPE
                     MGLLVLDPATRRRLRFNGRGAVERDGGFSLAVEQVYGNCRK"
     gene            477..1160
                     /locus_tag="VFD06_09170"
     CDS             477..1160
                     /locus_tag="VFD06_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HZN03741.1"
                     /translation="MSTPTQDIVIDAGEKSGAVAGLLMRPQSASSLLVFAHGAGAGMR
                     HAFMESAAELLGARGVATLRYEFPYMQRKSRRPDPPAVLQSTVRAAARAAAAAAPDLP
                     LLAGGKSMGGRMTSLAAAGEPLPGVRGLVFFGFPLHAAGSPSSERGAHLSRITVPMLF
                     LQGTRDRLADLDLVRPLLAGIPHANLKIFEGADHSFHVPKSAGRTDAEVLAEMTVTVA
                     DWAKTLRKD"
     gene            complement(1211..1528)
                     /locus_tag="VFD06_09175"
     CDS             complement(1211..1528)
                     /locus_tag="VFD06_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03742.1"
                     /translation="MTRTHGILVLVAVAFLMSAGGLTTPTITSQPAAPAAALVLAPVD
                     FSQGDVCRVASDMPPGEDHAEKVPPGCCSTQCNVDKDCNKICGKGVCACIQETSCCRR
                     CVY"
     gene            1673..>2222
                     /locus_tag="VFD06_09180"
     CDS             1673..>2222
                     /locus_tag="VFD06_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZN03743.1"
                     /translation="MPDKPFLTDIKTLRQRARQHIEQGAVTPGYKADRENVVRLLNEA
                     LATEIVCVLRYRRHHFMANGINAQSIAQEFLQHANEEQAHADQIAQRIVQLGGEPNFS
                     PEGLQTRSHAEYVEGDSLLDMIKEDLIAERIAIDSYREMIAYLGHDDPTTVRMLEGIL
                     AMEEEHADDLVTLLEELGKATAR"
BASE COUNT          376 a          761 c          754 g          331 t
ORIGIN      
        1 atttccggca gttgccgtac acctgctcga cggcgaggga gaacccgccg tcccgctcca
       61 ccgccccgcg gccgttgaaa cgcaggcgcc gacgcgtggc cgggtcgagg acgaggagcc
      121 ccatctcggg ccgcccggcc aggttggtcc gcagcggatc cccgggcgcg acccgggcac
      181 cgatgtggag gcgcgtctcg tcgacgatgt cgagaaaccc ggccggcccg gtcaggagcg
      241 aggcccagac gcgcccgcgg gggtcgatcg acgccgccac cgccaggcgg aactccgcca
      301 ggagcggacc gagatccatg gggatcgcgc cggcgatgat gcgtccgacg tcgtccgctc
      361 tgttccggac gccggcgcgt cgctggactt cgagctcgcc ggagtggtaa gtgggacgcg
      421 gctggctcat gaccctttgg atgccggaaa cggtgaagtg tgacgacacg ttccacatga
      481 gcactccgac acaggacatc gtcatcgacg cgggagaaaa gtccggcgcg gtcgccggcc
      541 tgctgatgcg cccgcagagc gcctccagcc tcctggtgtt cgcccacgga gccggcgccg
      601 gcatgcgcca cgcgttcatg gagtcggcgg cggagctgct cggggcgcgc ggggtcgcga
      661 ccctgcgcta cgagtttccc tacatgcaga gaaagtcgcg ccggcccgat ccgccggcgg
      721 tgctccagtc gaccgtgcgc gcggcggcga gagcggccgc cgcggccgcc ccggatctgc
      781 cgctcctcgc gggaggcaag tcgatgggag ggcgcatgac ctcgctggcc gccgccggcg
      841 agccgctccc gggtgtgcgc gggctcgtct tcttcggctt cccgctgcac gcagccggga
      901 gcccgtcgag cgaacgcggg gcgcacctgt cccgcatcac ggtgccgatg ctgttcctcc
      961 aggggacgcg cgatcggctg gccgacctcg acctggtccg cccgctgctc gcgggaatcc
     1021 cccacgccaa cctcaagatt ttcgaggggg ccgaccactc gttccatgtg ccgaaatcgg
     1081 cgggccgcac cgatgcggag gtgctcgccg agatgacggt tacggtcgcg gactgggcga
     1141 agacgctacg gaaggactga gacgcggacg cccggccgcc cctgcgagag aacggccggg
     1201 ccgcgaagcc tcagtacacg catctccggc agcacgacgt ttcctggatg caggcgcaga
     1261 cgcccttccc gcaaatcttg ttgcagtcct tgtccacgtt gcactgggtg gagcagcacc
     1321 ccggcgggac cttctccgcg tggtcctcgc ccggaggcat gtccgaggcc accctgcaca
     1381 cgtccccctg gctgaaatcg acgggggcga gcaccagcgc ggcggcggga gcggccggtt
     1441 gcgaggtgat cgtgggtgtc gtcaacccac cggccgacat cagaaacgcg acggcgacca
     1501 gaacgaggat tccatgtgtt cttgtcattc cggtttcctc ctctgctgct cttacagaga
     1561 cccgacgtgt ctgtcaaggc tccgctctcc acgtcgtcat ccggctcgct ggtggtcacc
     1621 gtcctgggct gatagcattc ttgcttcacg cgacgaaatc gaggagaagc cgatgcccga
     1681 caagccattc ctgaccgaca tcaagacctt gaggcagcgg gcgcgccagc acattgaaca
     1741 gggcgcggtg acccctgggt acaaggccga ccgtgagaac gtcgtccgtc tcctgaacga
     1801 ggcgctggcc accgaaatcg tgtgcgttct ccgctacagg cgtcaccatt tcatggcgaa
     1861 cgggatcaac gcccagagca tcgcgcagga gttcctccag cacgccaacg aggaacaggc
     1921 gcacgcggat cagatcgcgc agcggatcgt gcagctgggg ggcgagccga acttctctcc
     1981 ggaggggctc cagacccgca gccacgcgga gtacgtcgaa ggcgattccc tcctcgacat
     2041 gatcaaggag gacctgatcg ccgaacggat cgccatcgac agctaccgcg agatgatcgc
     2101 gtatctcggg cacgacgatc ccaccacggt gagaatgctc gaggggattc tcgccatgga
     2161 ggaagagcac gcggacgacc tcgtcacact gctggaggag ctggggaaag cgaccgcccg
     2221 gt
//
LOCUS       DASRIQ010000380         7419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1464104, whole genome shotgun sequence.
ACCESSION   DASRIQ010000380 DASRIQ010000000
VERSION     DASRIQ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464104"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VFD06_09185"
     CDS             complement(<1..1071)
                     /locus_tag="VFD06_09185"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03744.1"
                     /translation="MPRDPRAYLQRAEEHRRHGRLKEAIALSIEALGKHPGLDAVRVT
                     LGRAYLENAQPEEARTTLLEVFARLPEHHLAGKLLIESQRLLEDLGGAEATCRTLLEH
                     YPRDREIEPLLAEIVRARSKSAAEEAPAGPVAPAATAPPAAAAPARPGPSAVPAPPAA
                     TTQGPRAPAVAVAEPDPAPEYFPEDLAAAPGAPVPQPAAARAAAPPAAKPAAPPAVIP
                     SIAPTSSEASVAAPAAAQRVAAAPVPPIASVAAPARPASPAAPPSAGASKDALETNTL
                     AELYVKQGLPDRAIDVYRSMLRVEPGNAAAKRRLAELESAAPPAVTAAAARPAQPDRA
                     QPSGPDAKTSRTAAAPPATPPRP"
     gene            complement(1257..1739)
                     /locus_tag="VFD06_09190"
     CDS             complement(1257..1739)
                     /locus_tag="VFD06_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03745.1"
                     /translation="MRTDLTLTRLAALLGATVILAAALGCNDHDDPGQAENVVLVTSI
                     TVLGNSVASGIDTSATVGYSLNPRNPGNAATSFWHDVTLTSYNVSFEPAVIAPISGPI
                     STSFCLAGGACSVDIVLVPNGSKPGAGTTVVAHIAFEGRDTNDNPVNFNADAALVFIP
                     "
     gene            complement(1736..4285)
                     /gene="pilQ"
                     /locus_tag="VFD06_09195"
     CDS             complement(1736..4285)
                     /gene="pilQ"
                     /locus_tag="VFD06_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HZN03746.1"
                     /translation="MRLGKLAIVFTIAAAALHCSGNPPVSEPGQAALEVPSAVVVNAV
                     DHTATPSGTQVVLHGNYPFSFTTYQPEPRTLVVELLEVKAQGMNDDIRLDMPQVEGVA
                     VTNVQGVDGGNIAKFEFRNVQAARHSVRLEGNDLMIDFPTLSDTDNLPEPPLVAGDDP
                     DAMPGSEPLKPIAEPLAAETPDAPGAAPAPLPEAVPPPQDHKKGAPKPQASDPNVVRG
                     PEPSGPQARTLVSVSAEQGGAEPRVVLKADGAIREEHFELANPPRLVVDLLGVLDKTP
                     KEQKTTAVDSQVLTRVRVAQFATRPQPVARVVFDLKTPKAYAIEPTTGGIAVDFSEEA
                     VRRAAENPPMQPLASPLAAETPQAAPVEPKPEPEAETVAIPPGTEDAAPLPEPEKPAP
                     NPLLAQGDVKVESKPKEVPFKPAPTRNRAGGETLILAQRYQSRTISDTGPKYTGKKVS
                     FNFKDADLKDVFRLFHELTGYNIVLDPAVSGTLTIVLENVPEDQALDIILKNNGLDKV
                     FENNVIRIASTQKLAAEAAARKSLIEAQELEREPITFTRKLSYAKSKDVVPIIKKMMS
                     KRGDVIMDDRTNTLIITDLPDRQDPISRLIDNLDEQTPQVTIEARIVETDREFERDLG
                     ITWGAHAVADNAHGTQLPWSFPHTGSVDWDVNVPSPATAGRLGVSFGNVLDTFRLNAT
                     LDAFELNGDVKVLSSPRIATQNNQKATIEQGTQIPVVSTTATQINVEFISASLKLEVT
                     PQITREGTVIMDLKVDNSSPDFVNRVGDVPPILTERAQTQVLVRDGGTAVIGGIFKLN
                     DSVTTAGVPGLQRIPGLGWLFRSKSINRKNTELLIFITPRISKKAEAEENS"
     gene            complement(4282..4821)
                     /locus_tag="VFD06_09200"
     CDS             complement(4282..4821)
                     /locus_tag="VFD06_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03747.1"
                     /translation="MTRHARWGLALAVACAAAVGTTGIRAGDEPAAAPVADGTAAQQA
                     GETINRIMEDSEAVLAGRGFTYDPAGRRDPFRSLVDPLNAPAHGPRPRGIAGMLISEV
                     DLVGIVQKGKEAVAFFNGSDNKGYFLRVGDTLFDGKIIRIDRASGTVMFRQDVNDPRS
                     IKPYRDVTKRLASAEEESL"
     gene            complement(4818..5423)
                     /gene="pilO"
                     /locus_tag="VFD06_09205"
     CDS             complement(4818..5423)
                     /gene="pilO"
                     /locus_tag="VFD06_09205"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HZN03748.1"
                     /translation="MDFKKLPFWGQFAVVAGMGAILVGVAWTTYPNFNAMSKRNAEER
                     AKLEGLQTEIRKGQAIEAKLPEFEKEIENLQTKLNDLLAILPTTPETGELLKWVKNLT
                     DQSNLELKQFNPQALKAVEFYKEFPINMEIEGDYHDLGVFFDRISKYSRIINVSNVVI
                     GARTQAGKGSIHSTFTATTFVYDDKAAQAAEASQQEGGTKP"
     gene            complement(5436..6071)
                     /locus_tag="VFD06_09210"
     CDS             complement(5436..6071)
                     /locus_tag="VFD06_09210"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HZN03749.1"
                     /translation="MIKINLIAETQKGKSREKTPRLEGSGVLGQNVLMIGVVVLATLV
                     VGWRWTSLASEHRRLATEIVRAEQEKERLQAIIKKGEEYKAKKDLLQRKITLVSQLKR
                     NQSGPVHLLDEISKQLPDFLWLDNMNEASWSVQINGKATTYNAVSNFYNNLTGSRYFQ
                     NVVMGPITAIPAGVSFSLTCQFVPQTTAAAPEDAPTAAVPAEGPAQAPPGT"
     gene            complement(6068..7129)
                     /gene="pilM"
                     /locus_tag="VFD06_09215"
     CDS             complement(6068..7129)
                     /gene="pilM"
                     /locus_tag="VFD06_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HZN03750.1"
                     /translation="MFFSRSKSLIGLDIGSSAVKVVELKELGKGKGYQLQSVAVEPLS
                     PEAIVDGAIMDAGLVIEAIQKALTARKVKGADVAIALSGHSVIIKKISIPATSEEELA
                     EVIPWEAEQYIPFDVEDVNLAYQVLKGGGGDGNMDVLLVAAKKDKIHDYTSVVTQAGR
                     NPVLVDVDVFALQNCFEMNYDSDPGEGLALVNVGASTTNVAVLKGTTSIFWRDISVGG
                     NHYNDALRKELNVSFEQAESLKRGESADGVDTSRVPAILAAVNDFIGGEVQKTLDFFK
                     NTTPGESITRMVLAGGASRVPNLGEALAGRFGVPVEPLNPFQRVTPAKGISPETLDEV
                     SASVSIAVGLACRRVGDNR"
BASE COUNT         1012 a         2479 c         2596 g         1332 t
ORIGIN      
        1 cgggcgcggc ggcgtcgcgg gcggagccgc ggcggtcctt gacgtcttcg cgtcggggcc
       61 ggagggttgg gcgcgatccg gctgggccgg acgcgctgca gcggccgtga cggccggcgg
      121 cgcggcgctc tcgagctccg ccaggcgccg tttggccgcg gcgtttccgg gctcgacgcg
      181 cagcatgctg cggtacacgt cgatcgcgcg atccggcagg ccctgcttca cgtagagctc
      241 cgccagcgtg ttcgtctcga gggcatcctt cgaggcgccg gccgacggcg gggccgcagg
      301 cgacgcgggt cgagccggcg cggccacgct cgcgatcggt ggaaccggcg cggccgcgac
      361 gcgctgggcc gcagccggcg cggcgacgga ggcctcagaa gaagtcggcg cgatcgaagg
      421 gatcaccgcg ggaggagccg cgggcttcgc cgcgggcggc gcggcggcgc gggcggccgc
      481 cggctgcggg accggcgcgc ccggcgcggc ggcgaggtct tcggggaaat actccggcgc
      541 cgggtcgggt tcggccacgg cgaccgctgg agcgcgcggc ccctgcgtgg tcgcggcggg
      601 cggagccggc acggcggatg gaccgggccg ggccggggcc gccgcagcgg gcggagccgt
      661 ggcggcggga gcgaccggac cggccggggc ctcctctgcc gccgacttcg agcgggcgcg
      721 tacgatctca gcgagcagcg gctcgatctc gcgatcgcgc ggatagtgct cgaggagcgt
      781 gcggcaggtc gcctccgcgc cgccgagatc ctcgagcagt cgctgcgatt cgatcaggag
      841 cttgcccgcg aggtggtgct ccggcaggcg ggcgaacacc tcgagcagag tcgtgcgggc
      901 ctcctccggc tgggcgttct cgaggtaggc gcggccgagc gtcacgcgga ccgcgtcgag
      961 gccgggatgc ttgccgagcg cctcgatcga cagcgcgatc gcttccttga ggcgcccgtg
     1021 gcggcgatgc tcctccgccc gctgaagata ggcgcgcggg tccctcggca tcctctctcc
     1081 gtcagccttc ctggtccgga cgacccgccc tcacaccatc ctaactcttt gactcgcaag
     1141 cagtaatggc cgggcagccg ggcgacggcc gtgattatat ccgaaagccc cggaaacacg
     1201 acgcccccgg ggaagccccg ggggcgccgg attccacacg cggttcgaac ctcagcttac
     1261 gggatgaaca ccaaggccgc atcggcgttg aagttcaccg ggttgtcgtt cgtgtcccgc
     1321 ccctcgaaag cgatgtgcgc caccaccgtc gtgccggccc ccggcttgct gccgttgggc
     1381 accaggacga tgtcgaccga gcaggcgccg ccggccaggc agaaggaggt ggagatcggg
     1441 ccgctgatcg gcgcgatcac cgccggctcg aacgacacgt tgtaactggt gagcgtcacg
     1501 tcgtgccaga aggaggtggc ggcgttgccg gggttcctcg ggttcagcga gtagcccacg
     1561 gtcgcgctcg tgtcgatccc ggaggcaacg ctgttcccaa ggacggtgat cgaggtcacc
     1621 aggaccacgt tctccgcctg gcccgggtcg tcatgatcgt tgcagccgag ggccgcggcc
     1681 aggatcaccg tcgctcccag gagcgcggcg agccgggtca gagtgagatc ggtcctcatg
     1741 agttctcctc cgcttccgcc ttcttgctga tgcgcggtgt gatgaagatc aggagttcgg
     1801 tgttcttcct gttgatcgac ttgctgcgga acagccaccc gagcccgggg atgcgctgca
     1861 gcccggggac ccccgccgtg gtgaccgagt cattgagctt gaagatgccg ccgatgaccg
     1921 ccgtgccgcc gtcccggacc aggacctgcg tctgggcgcg ctcggtgaga atcggcggga
     1981 cgtcgccgac gcggttgacg aagtcgggcg acgagttgtc caccttcagg tccatgatga
     2041 ccgtcccctc gcgggtgatc tgcggggtga cctccagctt gagcgacgcc gagatgaact
     2101 cgacgttgat ctgggtcgcg gtcgtgctga cgaccgggat ctgcgtcccc tgctcgatcg
     2161 tcgccttctg gttgttctgt gtcgcgatgc gcggcgacga caggaccttc acgtcgccgt
     2221 tcagctcgaa cgcgtccagc gtggcgttca ggcggaaggt gtcgaggacg ttgccgaacg
     2281 acaccccgag gcggccggcg gtcgccggcg acgggacgtt gacgtcccag tcgacgctcc
     2341 cggtgtgcgg gaacgaccac ggcagctgcg tcccgtgcgc gttgtcggcg acggcgtgcg
     2401 ccccccaggt gatcccgagg tcacgctcga actcgcggtc ggtctcgacg atgcgcgcct
     2461 cgatggtgac ctggggcgtc tgctcgtcga ggttgtcgat cagccgggaa atcgggtcct
     2521 ggcggtccgg caggtcggtg atgatcagcg tgttggtccg atcatccatg atcacgtcgc
     2581 cgcgcttcga catcatcttc ttgatgatcg gcacgacgtc cttcgacttg gcgtaggaga
     2641 gcttgcgggt gaaggtgatc ggctcccgct ccagctcctg cgcctcgatg agggacttgc
     2701 gggccgccgc ctccgccgcc agcttctgcg tgctggcgat gcggatgacg ttgttctcga
     2761 agactttgtc gagcccgttg ttcttcagga tgatgtcgag cgcctgatcc tccggaacgt
     2821 tctcgagcac gatggtgagg gtgcccgaaa ccgccgggtc gaggacgatg ttgtagccgg
     2881 tcaattcgtg gaacaggcgg aagacgtcct tcaggtcggc gtccttgaag ttgaaggaga
     2941 ccttcttgcc ggtgtacttc ggtccggtgt cggagatggt cctcgactgg tagcgctgcg
     3001 ccaggatgag ggtctcgccg ccggccctgt tcctggtcgg cgccggcttg aacgggacct
     3061 ccttcggctt cgactccacc ttcacgtccc cctgcgccag gagcggattc ggcgcgggct
     3121 tctcgggctc cggcagcggc gcggcgtcct ccgtccccgg cgggatggcg accgtctccg
     3181 cctccggctc gggcttcggc tcgaccgggg cggcctgcgg cgtctcggcg gcgagcggtg
     3241 aggcgagcgg ctgcatgggc gggttctccg cggcgcggcg caccgcttcc tcggagaagt
     3301 cgacggcgat cccaccggtg gtcggctcga tggcgtacgc cttcggggtc ttgaggtcga
     3361 agacgacgcg cgccaccggc tggggccgcg tggcgaactg cgccacccgg acccgcgtca
     3421 gcacctgcga atcgaccgcg gtggtcttct gttccttcgg tgtcttgtcc agcaccccga
     3481 ggagatcgac gaccaggcgc ggcgggttgg ccagctcgaa gtgctcctcg cggatcgcgc
     3541 cgtcggcctt gagcaccacg cgcggctccg ccccgccctg ctcggcgctc acgctgacga
     3601 gggtgcgggc ctgcgggccg ctcggctcgg gcccgcggac gacgttcgga tcggaggcct
     3661 gcggcttcgg ggcccccttc ttgtggtcct gcggcggcgg gaccgcctcc ggcagaggag
     3721 cgggagccgc ccccggcgcg tccggggtct cggccgccag cggctcggcg atcggcttca
     3781 gcggctccga tccgggcatc gcgtcggggt cgtcgccggc gacgagcggc ggctccggca
     3841 ggttgtcggt gtcgctgagg gtcgggaagt cgatcatcag atcgttcccc tccagccgca
     3901 ccgagtgccg cgccgcctgt acgttgcgga actcgaactt ggcgatgttg ccgccgtcca
     3961 caccctggac attggtcacc gccaccccct cgacctgggg catgtccagg cggatgtcgt
     4021 cgttcatccc ctgcgccttc acctcgagca gctccaccac cagtgtccgg ggttccggct
     4081 gataggtggt gaagctgaac gggtagttcc cgtgcagcac cacctgcgtg ccggacggcg
     4141 tggcggtgtg atcgacggca ttcaccacca ccgccgacgg cacctcgagt gccgcctggc
     4201 ccggctcgct cacgggcggg ttgcccgagc agtgcagggc ggccgcggcg atcgtgaaca
     4261 caatcgcgag cttgccgagc ctcatagact ctcctcctcc gctgacgcga gccgcttggt
     4321 gacatcccgg tagggcttga tgctccgggg atcgttgacg tcctgccgga acatcaccgt
     4381 ccccgaggcg cgatcgatgc ggatgatctt gccgtcgaag agcgtgtcgc ccacgcgcag
     4441 gaaatacccc ttgttgtcac tgccgttgaa gaaggccacc gcctctttcc ccttctgcac
     4501 gatgccgacg aggtcgacct cggagatgag cattccggcg atgccgcgcg gccgcggtcc
     4561 gtgcgccggc gcgttgagcg gatcgacgag ggagcggaac gggtcgcggc gcccggccgg
     4621 gtcgtaggtg aaaccgcgcc cggcgagcac cgcctcgctg tcctccatga tccggttgat
     4681 ggtctcgcct gcctgctggg ccgccgtgcc gtcggcgacg ggggccgccg ccggctcgtc
     4741 gccggcccgg atccccgtcg tccctaccgc ggcggcgcac gcgacggcga gcgccaggcc
     4801 ccaacgcgcg tgacgcgtca tggcttcgtt cctccctcct gctgcgatgc ctctgccgcc
     4861 tgggccgcct tgtcgtcgta cacgaaggtg gtggcggtga acgtcgagtg gatcgacccc
     4921 ttgccggcct gggtccgggc gccgatcacc acgttgctga cgttgatgat ccgggagtac
     4981 ttgctgatgc gatcgaagaa gacgccgagg tcgtggtagt ccccttcgat ctccatgttg
     5041 atcgggaact ccttgtagaa ctccaccgcc ttcagcgcct gcgggttgaa ctgcttcagc
     5101 tcgaggttcg actggtcggt gaggttcttg acccacttga ggagctcgcc ggtctccggc
     5161 gtcgtcggca ggatggccag caggtcgttc agcttggtct ggaggttctc gatctccttc
     5221 tcgaactccg gcagcttcgc ctcgatcgcc tgccctttgc ggatctcggt ctgcagcccc
     5281 tcgagcttgg cgcgctcctc ggcgttgcgt ttcgacatgg cgttgaagtt cgggtacgtg
     5341 gtccaggcca cgccgaccag gatcgccccc atgccggcga cgaccgcgaa ctgaccccag
     5401 aacggcagct tcttgaagtc catcgtccct cccccctagg tcccgggcgg ggcctgagcg
     5461 ggcccctccg ccgggacggc cgccgtcggc gcgtcctcgg gggcggcggc ggtcgtctgc
     5521 ggcacgaact ggcaggtcag cgagaacgac acgccggcag gaatggcggt gatcggcccc
     5581 atgaccacgt tctggaagta ccgcgagccg gtcaggttgt tgtagaagtt cgagacggcg
     5641 ttgtaggtcg tggcctttcc gttgatctgc acgctccagg acgcctcgtt catgttgtcg
     5701 agccagagga agtcggggag ctgcttgctg atctcgtcga ggaggtggac cgggccgctc
     5761 tggttccgct tcagctgcga caccagcgtg atcttccgct gcagcaggtc cttcttcgcc
     5821 ttgtactcct cccccttctt gatgatcgcc tgcaggcgct ccttctcctg ctcggcgcgg
     5881 acgatctcgg tcgccaggcg gcggtgctcg ctcgcgaggc tggtccagcg ccagccgacg
     5941 accagcgtcg ccaggacgac gacaccgatc atcaggacgt tctggccgag gacgcccgat
     6001 ccctcgaggc ggggggtctt ctcgcggctc ttccccttct gcgtttcggc gatgaggttg
     6061 atcttgatca tcgattgtcc ccgacccggc ggcaggcgag cccgacggcg atggaaacgc
     6121 tggcgctgac ctcgtcgagg gtctcggggg agatcccttt cgccggggtg acgcgctgga
     6181 acggattgag cggctcgacc gggacgccga agcggccggc cagggcctcg cccaggttcg
     6241 gcaccctcga ggcgccgccc gcgaggacca tgcgggtgat gctctcgccg ggggtggtgt
     6301 tcttgaagaa gtcgagggtc ttctgcacct cgccgccgat gaagtcgttc accgcggcga
     6361 ggatggcggg cacgcgcgag gtgtcgacgc cgtcggcgct ctcgccgcgc ttgagcgatt
     6421 ccgcctgctc gaagctgacg ttcaactcct tgcgcagcgc gtcgttgtaa tggttgccgc
     6481 cgaccgagat gtcgcgccag aagatcgagg tggttccctt cagcaccgcg acattggtgg
     6541 tggaggcgcc gacgttgacc agcgccaacc cctcgccggg gtcggagtcg tagttcatct
     6601 cgaagcagtt ctgcagcgcg aagacatcga cgtccacgag gaccgggttg cgcccggcct
     6661 gggtgacgac gctggtgtag tcgtgaatct tgtccttctt cgcggcgacg agcaggacgt
     6721 ccatgttgcc gtcccccccg ccccccttca gcacctggta ggcgaggttg acgtcttcga
     6781 cgtcgaacgg gatgtactgc tccgcctccc acgggatgac ctcggccagc tcctcctcgg
     6841 acgtggccgg aatcgagatc ttcttgatga tgacgctgtg acctgagagg gcgatggcga
     6901 cgtccgcccc cttcaccttc cgcgccgtga gcgccttctg gatcgcctcg atgaccaggc
     6961 cggcgtccat gatggcgccg tcgacgatcg cctcggggga gagcggctcg acggcaacgc
     7021 tctggagctg gtatcccttc ccttttccga gctccttgag ctcgacgacc ttgacggcgc
     7081 tcgagccgat gtcgagcccg atgagcgact tcgatcgcga gaagaacacg gccttccctc
     7141 ccctctggtg ctcaagcggc cccctcgtgg agggggctcg tttttcacga atctccaata
     7201 tattccgcga cctcacttct gcaaggcgct ctatcaaaac tgaacactca ttattgcagc
     7261 gatgaatatg tctatctgcg tggatcgacg caacgtgttg aaatcaagcg acttcggtgg
     7321 atcggcgagg gtcctgcggg gaaacgtgtt gtgcttgagc cggccaccac gaatgtgcta
     7381 gagttcccga cttccgtaac ggatcaaccc gggcgcaag
//
LOCUS       DASRIQ010000381         4914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_936164, whole genome shotgun sequence.
ACCESSION   DASRIQ010000381 DASRIQ010000000
VERSION     DASRIQ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936164"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFD06_09220"
     CDS             <1..560
                     /locus_tag="VFD06_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZN03751.1"
                     /translation="PGAGTAGGTEAADAERLLRDEEEAIGYLISRHPLTLYREAIARL
                     RREGVALVRGADIGKHAGARVAMIGWLVTGKLVTTKDDEPMEFVSFEDTTAIYETVFF
                     PEAYARFCRMLTTARPYLLRGKVEEEFGVPSLQVEEVEFLRRTAPKPGVSPAVSGPRG
                     AAGLGAARPGSPRSSPSGRFSRTPA"
     gene            complement(454..1719)
                     /locus_tag="VFD06_09225"
     CDS             complement(454..1719)
                     /locus_tag="VFD06_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN03752.1"
                     /translation="MRRLTGLLLLVAGACAGAAIALLVIPAGAAGIPLLRPAAEPGSA
                     GPAGGPAQAPSGSRAARGGPVAAAGAIPAASLAAGDGYAAIARSVMPAVVNISSMQVI
                     QTYERYSPFMADPFFQQFFGGGVYDYVVPRERREMSLGSGVIVDDHGTILTNHHVVEH
                     ATEVRVALSDGRELPARILGVDPRTDLAALRVEGPNLPHAPLGDSDSMQVGDIVLAFG
                     NPFGLGGTVTMGIVSAIGRGNLGLADYEDFIQTDAAINPGNSGGALVNTRGEVIGINT
                     AIFSRSGGYQGIGFAIPSTMAHEVLDDLLDHGRVVRGYAGLALQPLTPDLARAFNLPD
                     LRGTVVADLDPNGPCARAGLKRGDVIVSVGSRLVASDEELRTQMSRLRPGDKVTLGFV
                     RTGRKATTEVTLVEPPQAPQRRAVPRRRG"
     gene            1848..2537
                     /locus_tag="VFD06_09230"
     CDS             1848..2537
                     /locus_tag="VFD06_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZN03753.1"
                     /translation="MLETLLNDIGPLLRDYLPKALLAIACGGLIGVEREIKQKPAGFR
                     TNILICVGSALFMWLSIHLVADLTGLRGDPGRIAAQVVAGVGFLGAGTILHSRGHITG
                     LTSAAMIWVVSAIGMAIGAGQTSVALFTTGLILLVQVGLGLVERRLFGRCRMRDCEIS
                     FDDDRGQTRREIEKSIRAAGQGQALESTGLRRAADHHVLTLQCCGVHPQHKKFIGDLW
                     KIDGVREVRFV"
     gene            2683..2823
                     /locus_tag="VFD06_09235"
     CDS             2683..2823
                     /locus_tag="VFD06_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03754.1"
                     /translation="MADEIYQRVKEAFQAAMEHAPSERSEDKKNREYRLLLAEAKGLL
                     FP"
     gene            complement(2836..4026)
                     /locus_tag="VFD06_09240"
     CDS             complement(2836..4026)
                     /locus_tag="VFD06_09240"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HZN03755.1"
                     /translation="MFDAPFRPALAAAAALAGLLAVPAPLRAVDTGSIDGTVEDARGR
                     PIPGAAVTLTAKRGGHALGRTDENGFFRIPAQTTAQQFILKVDADGFRPVAYDGLQLM
                     AGRTRRFDVRLKRPGERDVVVLLSRDPYPFDDLLRGLLQGLDAPARTFDLDTVPDPEE
                     VLRRVEAERPNLILASGLMAARLVRREVRDIPAVLSLLGDPRLHDLDAANLSYVTTNA
                     PPADLIGRVRQFLPEARRLGLLFDARASTLVARDLRRAARHHGFDVVTRPCYEPEKIE
                     QALADLAGKVDVLVVPFDPLSLSPSALDDVTHWSLSQRVALVGPGPEWVRRGALFSYG
                     PTPETIGRDLSLLAGRMLYEGFQPEGDARLIGPGGSVLTVNTTTALALGVEIPGSVSV
                     DATY"
     gene            4185..>4914
                     /locus_tag="VFD06_09245"
     CDS             4185..>4914
                     /locus_tag="VFD06_09245"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZN03756.1"
                     /translation="MTQPLMRTEFDDLELLSRGKVRDVYDLGDALLIVSTDRISAFDH
                     ILPNGIPDKGKVLNQISLFWFDRTREIVRHHVRERDASRYPTRLRPHVATLRGRSVVV
                     ERLDMLPVECVVRGYLSGSGYKEYLKTGAVCGVKLPAGLRDSDRLPEPIFTPSTKATT
                     GHDENISFDKVVDLVGRSTAEAVRDLSLAVYDHACRHAESRGIIIADTKFEFGKDARG
                     LVLGDEVLTPDSSRFWPADTYKPGG"
BASE COUNT          758 a         1676 c         1761 g          719 t
ORIGIN      
        1 caccgggtgc gggaaccgca ggcgggaccg aggcggctga cgccgagcgg ctcctgcgcg
       61 acgaggaaga ggcgatcggc tacctcatca gccgccaccc gctgacgctg taccgcgagg
      121 cgatcgcgcg gctcaggcgg gaaggcgtgg cgctggtgcg cggcgccgac atcgggaagc
      181 acgcgggggc gcgggtggcg atgatcggct ggctcgtcac cgggaagctg gtcacgacca
      241 aggacgacga gccgatggag ttcgtctcct tcgaggacac gaccgccatc tacgagacgg
      301 tcttcttccc ggaggcgtac gcgcgattct gccggatgct gacgacggcg cgaccttatc
      361 tgctgcgcgg taaagtggag gaggagttcg gcgtcccgag cctgcaagtc gaggaggtcg
      421 agttcctgcg ccgcactgcc ccaaagccgg gggtcagccc cgccgtctcg ggaccgcgcg
      481 gcgctgcggg gcttggggcg gctcgaccag ggtcacctcg gtcgtcgcct tccggccggt
      541 tctcacgaac cccagcgtga ccttgtcccc gggccgaagc cgcgacatct gcgtgcgcag
      601 ctcctcgtcg gaagcaacca ggcgcgagcc gaccgagacg atgacatcac cgcgcttcag
      661 accggcgcgc gcgcaggggc cattcgggtc gaggtccgcc acgacggtgc cgcgcaggtc
      721 gggaagattg aaggcgcgcg ccaggtccgg cgtcagcggc tgcagcgcga gcccggcgta
      781 gccgcggacc acgcggccgt ggtcgaggag gtcgtcgagc acctcgtgcg ccatcgtgga
      841 cgggatggcg aagccgatcc cctggtaccc gccactgcgc gaaaagatcg ccgtgttgat
      901 gccgatgacc tcgccacgcg tgttgactag ggcgccaccg gagttgccgg gattgatggc
      961 ggcgtccgtc tggatgaagt cctcgtagtc ggcgagcccg aggttgccgc ggccgatggc
     1021 gctgacgatc cccatcgtca cggtcccgcc gaggccgaac gggttgccga acgccaggac
     1081 gatgtcgccg acctgcatgc tgtcggagtc cccgagcggc gcgtgcggca ggttcggccc
     1141 ctcgaccctg agcgccgcga ggtcggtacg cggatcgacc ccgaggatgc gggccggcag
     1201 ctcgcggccg tccgagagcg ccacccgcac ctcggtcgcg tgctcgacca cgtggtggtt
     1261 ggtcaggatc gtcccgtggt cgtccacgat gacccccgac ccgaggctca tctcgcgccg
     1321 ctcgcgcggc accacgtagt cgtacactcc gccgccaaag aactgctgga agaaggggtc
     1381 ggccatgaac ggggagtagc gctcataggt ctggatcacc tgcatcgagg agatgttcac
     1441 gaccgccggc atgacacttc tggcgatggc cgcgtagccg tcgccggcgg cgaggctcgc
     1501 ggcggggatg gcgccggcgg ccgcgaccgg gccaccgcga gcggcgcggg aacccgaagg
     1561 ggcctgcgcg ggcccccccg cagggccggc ggatccgggc tctgccgcgg gccgcaagag
     1621 ggggatgcct gctgccccgg ccggaatcac caggagggcg atggcggcgc cggcgcaggc
     1681 ccccgccacc aggagcagga gaccggtcag ccggcgcaag gccgtgtctc cacgaaagcg
     1741 gagtccggcg gtcgatgacg ggtgggtgga catccctggt tccggccgcg ggtcattcga
     1801 cttgcaccgc gagggggcgg attatacctt tgccgtcgtc ttcaaccatg ctcgaaaccc
     1861 ttctcaacga catcggccct ctccttcgag actacctccc gaaggcgctt ctggcgatcg
     1921 cgtgcggcgg cctcatcggc gtcgagcgcg agatcaagca gaagccggcg ggattccgca
     1981 ccaacatctt gatctgcgtc gggtcggccc ttttcatgtg gctgtcgatt cacctcgtcg
     2041 ccgacctcac cggcctgcgc ggcgatccgg ggcgcatcgc ggcgcaggtc gtcgccggcg
     2101 tcggttttct cggcgccggg accatcctgc actcgcgcgg ccacatcacc gggctgacgt
     2161 cggccgcgat gatctgggtg gtgtcggcca tcggtatggc gatcggagcc gggcagacat
     2221 cggtcgcgct gttcaccacc gggctcatcc tgctcgtgca ggtggggctc gggctcgtcg
     2281 agcggcggct cttcgggcgc tgccgcatgc gcgactgcga gatctccttc gacgacgatc
     2341 gcgggcagac gcggcgggag attgagaagt cgattcgcgc cgcgggccag gggcaggccc
     2401 tcgagtcgac cggcctcaga cgtgccgccg atcatcacgt cctgacgctg cagtgctgcg
     2461 gggtccaccc gcagcacaag aagttcatcg gcgatctctg gaagatcgac ggcgtgcgcg
     2521 aggtgcggtt cgtctagctc gatcgaactc ctcgtgttca tcgggttaaa tggcttttcc
     2581 tacccctcac ttctccacgc gagaaaccgg ccccgcgcgc atcagggact cgtcacacgg
     2641 catcctgggc taccatgggc cgaatcaagc ggcgggcgcg ccgtggccga cgaaatctat
     2701 caacgtgtca aagaagcgtt ccaggcggcg atggagcacg cgccgtcgga acgctcggaa
     2761 gacaagaaga atagggagta ccggcttctt cttgccgaag cgaagggcct cctgttcccc
     2821 tgaagcggag gatggtcagt aggtcgcgtc gacgctgacg ctgccgggaa tctcgacgcc
     2881 gagggccagc gccgtcgttg tgttcaccgt caggaccgag ccgcctggcc cgatgagccg
     2941 cgcgtccccc tccggctgga acccttcgta gagcatccgt cccgcgagaa gggacaggtc
     3001 ccgcccgatc gtctccgggg tcgggccgta cgagaacagg gcgccgcgcc gcacccactc
     3061 cggccccggg cccacgagcg ccacgcgctg cgacagcgac cagtgggtca cgtcgtcgag
     3121 ggcgctcggg ctgaggctca gcgggtcgaa cgggacgacg agcacgtcca ccttcccggc
     3181 gaggtcggcg agcgcctgct cgattttctc cggctcgtag caggggcggg tgacgacgtc
     3241 gaacccgtgg tggcgcgccg cgcgccgcag gtcgcgcgcg acgagggtcg aggcgcgcgc
     3301 gtcgaacaga aggccgagcc gccgcgcctc cggcaggaac tgacgcaccc gcccgatcag
     3361 gtcggcgggc ggggcgttgg tcgtgacgta ggacaggttc gcggcgtcga gatcgtgcag
     3421 gcgcgggtcg ccgaggaggc tcagcaccgc cgggatgtcg cggacctcgc ggcgcaccag
     3481 gcgcgccgcc atgagtccgg aggcgagaat gagattaggg cgctccgcct cgacgcggcg
     3541 cagcacctcc tccggatcgg ggaccgtgtc gagatcgaag gttcgcgccg gcgcatcgag
     3601 cccctgcagc aggccgcgca gcaggtcgtc gaacgggtac gggtcgcggc tgaggagaac
     3661 gaccacgtcc cgctctccgg gccgcttcag gcggacatcg aagcggcgcg tccgcccggc
     3721 catcagctgc agcccgtcgt acgcgaccgg ccggaagccg tccgcgtcca ccttcaggat
     3781 gaattgctgc gcggtggtct gtgccgggat gcggaagaat ccgttctcgt cggttcggcc
     3841 cagcgcgtgc ccgccccgct tcgccgtcag cgtcaccgcg gcgccgggga tcgggcggcc
     3901 gcgcgcatcc tcgaccgtcc catcgatcga ccccgtgtcg accgcgcgga gcggcgcggg
     3961 gaccgcgagc agccccgcca gggcggcggc cgccgcgagg gcggggcgga aaggagcgtc
     4021 gaacatgacc ctcgacggta ccgcgtggcc gagccgcggt caacccgccc cgccgctcgt
     4081 cgggcgcggc gggagggcgg atcgggcggc gtttgcgtgg tcttttcgtt gacacgaaat
     4141 gggaggcatg caaggattcc gcctcttcaa cacgaggcca cgacatgacg cagccactga
     4201 tgcgcaccga gttcgacgac ctcgaactgc tcagccgcgg caaggtccgg gacgtgtacg
     4261 acctggggga cgcgctcctc atcgtctcga ccgatcggat ctccgcgttc gatcacatcc
     4321 tgccgaacgg cattccggac aaagggaagg tcctcaacca gatttcgctc ttctggttcg
     4381 accggacgcg cgagatcgtc cggcaccacg tgcgcgagcg tgacgcctcc cggtacccta
     4441 cgcgcctgcg accgcacgtc gcgacgctgc gcggccgttc ggtcgtggtt gagcggctcg
     4501 acatgctgcc ggtcgagtgc gtcgtgcgcg gctacctgtc gggctccggg tacaaggagt
     4561 acctgaagac cggagcggtc tgtggggtca agctgccggc cgggctgcgc gactccgacc
     4621 gcctgccgga gccgatcttc accccctcga ccaaggcgac gaccggccac gacgagaaca
     4681 tctccttcga caaggtggtc gatctcgtgg ggaggtcgac ggccgaggcg gtgcgcgacc
     4741 tgagcctcgc cgtctacgac cacgcctgcc ggcacgccga gagccgcggg atcatcatcg
     4801 ccgacacgaa gttcgagttc gggaaagacg cgcgcggact cgtgctcggg gacgaggtgc
     4861 tcacgccgga ctcgtcccgc ttctggccgg ccgatacgta caagccgggc gggc
//
LOCUS       DASRIQ010000382         4419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_160858, whole genome shotgun sequence.
ACCESSION   DASRIQ010000382 DASRIQ010000000
VERSION     DASRIQ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160858"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFD06_09250"
     CDS             <1..290
                     /locus_tag="VFD06_09250"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZN03757.1"
                     /translation="ENRTPLPDEVATCSPFLFRQIAAIRPKVIVCLGTPSAQAVLGTR
                     ETITRLRGTFRDVGGYRVMPTFHPAYLLRNPAAKREVWDDLKQVMAALAES"
     gene            294..2798
                     /gene="priA"
                     /locus_tag="VFD06_09255"
     CDS             294..2798
                     /gene="priA"
                     /locus_tag="VFD06_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZN03758.1"
                     /translation="MPDLTAGRVSVAVPGPLRRLWSYSVPGPLRDACRPGTRVLVPFG
                     GRRVTGVVVGVEPVSHDEPEAFALKPVERVLDTAPALDAGLLELTRWAADYYGASWGE
                     MIRTALPGQQAVVRLEAALTVAGEEALAAAAALDATGSTAKSGAATRGREARRVLAAV
                     AETAGAPGAFVSLDRLRRAGLEVSPARARALARSGLLAVREAVHRGGPEPRLETWVTA
                     TAQPGPEVKGRRQRAALEALAAHSQGMSAADLHAASGAGAAVLRSLATRGLVQVTTKA
                     CSPRTSTPAAAPGGAEAPHRLTPAQQEALDTVSAAVAAKRFEAFLLFGVTGSGKTEVY
                     LRACEAALAAGRSALYLVPEIGLTPLLARQMRERFGARLALLHSGLPEGERQDTWREA
                     RAGRVRVVLGARSAVFAPLQDLGIVVVDEEHDTSYKQDEHPRYNGRDLAIVRARGCGA
                     AALLGSATPAVETYFRARTGKFRLLRLEGRAGGAALPLVTRVDMRQEFEETGRDVVLS
                     RRLRAALEERLRRGEQSLVLLNRRGFATFALCRACGKPIECDACSIGMTLHLKEKRLR
                     CHYCNAARGVPAACPACGGGPMHFGGTGTERLEETIRSLFPRARVARMDRDTVRGRGA
                     AERLLLDVERGEIDILLGTQMIAKGHDFPNVTLVGVLAGDALLGFPDFRAGERTFQLL
                     AQVAGRSGRRLAPGEVIVQAYDPGHHAVRAACEHDYEGFAREELRYRKALHYPPYSAL
                     ALLVFRDARYEAALQAAARIVAGIKGRGDAEVQVLGPAPAPIERLRGQWRVQVLLKGA
                     RRAAVKSALNDAVGRIEAEGLRPDAVGIDVDPVSTL"
     gene            complement(2806..3795)
                     /gene="selD"
                     /locus_tag="VFD06_09260"
                     /pseudo
     CDS             complement(2806..3795)
                     /gene="selD"
                     /locus_tag="VFD06_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            3950..>4419
                     /locus_tag="VFD06_09265"
     CDS             3950..>4419
                     /locus_tag="VFD06_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZN03759.1"
                     /translation="MPPNPPACDSILGTVGRTPLVLLRRLNPHPDVRIWAKLEGFNPM
                     GSVKERIALRIVEEAERRGALRPGMTLLESSSGNTGIGLAMVGAVKGYPVVITMAQKV
                     SRERRQILHALGAQVVFTSKDGGSDEAWAMADRMQREEPERYFRIGQYESPDNPL"
BASE COUNT          588 a         1562 c         1676 g          593 t
ORIGIN      
        1 ccgagaaccg gacgccgctg cccgacgagg tcgccacctg ctcgccgttc ctcttccggc
       61 agatcgccgc catccggccg aaagtgatcg tctgcctcgg gacgccgtcg gcgcaggcgg
      121 tcctcgggac gcgcgagacg atcacccgcc tgcgcgggac gttccgcgac gttgggggct
      181 accgcgtcat gccgaccttc caccccgcgt acctgctgcg gaatcccgcc gccaagcggg
      241 aggtctggga cgatctcaag caggtgatgg ccgccctggc cgagtcgtag ccggtgccgg
      301 acctgacggc ggggcgcgtc agcgtggcgg tgcccgggcc gctgcggcgg ctgtggagct
      361 actcggtgcc gggcccgctg cgcgacgcgt gccggccggg gacgcgcgtc ctggtcccgt
      421 tcggcggccg acgcgtcacc ggcgtcgtgg tcggcgtcga gccggtctcc cacgacgagc
      481 ccgaggcctt cgctctcaag ccggtcgagc gcgtgctcga cacggcaccg gcgctcgacg
      541 ccgggctcct cgaactgacc cgctgggccg ccgactacta cggcgcctcc tggggggaga
      601 tgatccggac cgcccttccg ggccagcagg cggtggtgcg cctcgaagcg gcgctgaccg
      661 tggcgggaga ggaggcgctg gcggcggccg cggcgctgga cgcgaccgga agcacggcga
      721 agtccggagc ggcgactcgc ggccgcgagg cgcggcgggt tctcgccgcg gtggcggaga
      781 ccgccggcgc tccaggggcc ttcgtctcgc tcgatcggct ccgccgcgcg gggctggagg
      841 tgtcgcccgc ccgggcgcgc gcgctggcgc gatcgggact cctcgcggtc cgcgaggcgg
      901 tgcaccgcgg cggtccggag ccgcgcctcg agacgtgggt gaccgccacg gcccagccgg
      961 ggccggaggt gaaggggcgc aggcagcggg cggcgctcga ggccctcgcc gcgcactcgc
     1021 aggggatgag cgcggcggat cttcacgccg cgtccggcgc cggcgccgcg gtcctgcgct
     1081 cgctcgcgac gcgcgggctc gtgcaggtca cgacgaaagc gtgttccccg cgcacttcga
     1141 cgcccgccgc ggcgccgggc ggggcggagg cgccgcatcg tctcacgccg gcgcagcagg
     1201 aggcgctcga cactgtctcc gccgccgtgg cggcgaagcg cttcgaggcg ttcctgctct
     1261 tcggcgtcac cggcagcggc aagaccgagg tgtatctccg ggcctgcgaa gcggcgctcg
     1321 cagccggacg cagcgccctc tatctcgttc cggagatcgg cctgacgccg cttctggcgc
     1381 ggcagatgcg ggagcggttc ggggcccggc tcgcgctgct ccactccggc ctgccggagg
     1441 gcgagcgcca ggacacctgg cgggaggcgc gcgcggggcg cgtccgggtg gtgctcgggg
     1501 cccgatcggc ggtcttcgcg cccctgcagg atctcggaat cgtggtggtc gacgaggagc
     1561 acgacacctc ctacaagcag gacgagcacc cccgctacaa cgggcgcgac ctggccatcg
     1621 tccgggcgcg cggctgcggg gcggcggccc tcctcggctc ggcgacaccg gcggtcgaga
     1681 cgtacttccg ggcgcgcacc gggaagttcc gcctgctgcg cctggaggga cgcgccggcg
     1741 gggcggcgct tccccttgtg acgcgcgtcg acatgcggca ggagttcgag gagacgggcc
     1801 gggatgtcgt cctgtcccgg cgcctccggg ccgcgctcga ggagcgcctg cgccgcggcg
     1861 agcagagcct cgtgctgctc aaccggcgcg gcttcgcgac cttcgccctg tgccgcgcct
     1921 gcggcaagcc gatcgaatgc gacgcgtgca gcatcggcat gacgctgcac ctcaaggaga
     1981 agcgcctgcg ctgtcactac tgcaacgccg cccggggggt cccggccgcc tgcccggcgt
     2041 gcggcggcgg gccgatgcat ttcggcggaa ccggcaccga gcggctggag gagacgatcc
     2101 gctcgctctt cccgcgggcg cgggtcgccc gcatggatcg tgacacggtg cgcgggcgcg
     2161 gcgccgcgga gcggctgctg ctcgatgtcg agcggggaga gatcgacatc ctgctcggca
     2221 cgcagatgat cgccaagggg cacgacttcc ccaacgtcac gctcgtcggc gtgctggcgg
     2281 gggacgcgct gctcggcttc cccgatttcc gggcgggcga gcggaccttc cagctcctgg
     2341 cgcaggtcgc ggggcggagc ggccggcgcc tggcgccggg agaggtgatc gtgcaggcgt
     2401 acgatcccgg ccaccacgcg gtgcgggcgg cctgcgagca cgactacgaa ggcttcgcgc
     2461 gcgaggagct gcgctaccgt aaggcgctcc actacccgcc ctacagcgcc ctcgcgctcc
     2521 tggtcttccg cgacgcgcgc tacgaggcgg cgctgcaggc ggccgcgcgg atcgtcgcgg
     2581 ggatcaaggg gcggggggac gcggaggtgc aggtcctcgg cccggcgccg gcgccgatcg
     2641 aacggctgcg cggccagtgg cgcgtgcagg tgctcctgaa gggggcgcgg cgcgcggcgg
     2701 tgaaaagcgc actgaacgat gcggtcggcc ggatcgaggc cgaggggctc cgtccggacg
     2761 ccgtcggtat cgacgtcgat ccggtgtcga ccctctagcg gcggatcacc ggacgaactc
     2821 gagcggcggg cccccggccg gcagcacctc ggcgatcgcc gccgcgtcgg tcacgccgcg
     2881 ctcacgcaga cgcgccacga agggggcggc ctgtgacgcg ggcagcgcga cgaggagccc
     2941 tccggacgtc tgcggatcga cgaggagccc gcgttcggcc tcaccgaagg caccgttgaa
     3001 gcgcatccga ccctccaccg cctccaggtt cgacccggtc accgacgtcg tgacgcccga
     3061 ggcggcgagc gccaaagcct cctcgtagcg cggcaggtcg tcgaagcgga tccggaagcg
     3121 cacgccgtcg cgggtcatgc cgagtgcgtg gccggcgaag ccgaagccgg tgatgtcggt
     3181 gcaggcgtgt ggctcgaact ggagcatcgt ctcgcacgcg gtgcggttga gcgccgtcat
     3241 ccgggcgacg gcgcgttcga gcaactcgtc gctgacgagc ccgcggcggc gtcccccgac
     3301 gatgagcccg gtgccgatcg gcttggtgag caccagcgcg tcgcccggcc gcgccccgat
     3361 gttgcgcagg atccggcgcg gatcgacgag gccggtcacc gacaggccgt acttcagctc
     3421 gtcgtcgcga acggtgtggc cgccgacgag cgtcgccccg gcttcgcgga tcttggtgta
     3481 cccgccttcg aggatgcggc gcagatcgtc cttgtggatt ccctttgccg ggaagttgca
     3541 gcagttcatc gccgtgagcg gccggccccc catggcgtag acgtcggaga tcgagttggc
     3601 ggcggccacc tggccgaaca catagggatc gtcgacgacc ggagtgatgt aatcgaccgt
     3661 ctgcacgagc gcgagatcgt cgccgagccg gtacacaccc gcatcgtcac cggtctcgaa
     3721 gccgacgaga aggttcggat cggtcttcgc gtccacggcg aattcacgga cgatgtcgct
     3781 caagtccacc ggaccgagtt tggccgctca accgccggag cgggcgtgac tcgtcagcat
     3841 ggaggactga cgctcgctca ttccacctcg cacagtgcgc aggtcgcgcg cggcggccat
     3901 cgtagcacgc gccccctcga gcctccggcg tgtgtagaat cagccctcca tgccgccgaa
     3961 cccgcccgcc tgtgacagca tcctcggcac cgtcgggagg acgccgctcg tgctgctgcg
     4021 tcgcctcaac ccgcacccgg acgtgcggat ctgggcgaaa ctcgaggggt tcaacccgat
     4081 ggggagcgtc aaggagcgga ttgccctgcg catcgtcgag gaggcggagc ggcgcggcgc
     4141 gctgcgcccg ggcatgaccc tgctcgagtc gtcgagcggc aacaccggca tcgggctggc
     4201 gatggtcggg gcggtgaagg gatacccggt cgtcatcacc atggcgcaga aggtgtcgcg
     4261 cgagcggcgc cagatcctgc acgcgctcgg ggcgcaggtg gttttcacct cgaaggacgg
     4321 cggctccgac gaggcctggg cgatggccga ccggatgcag cgggaggagc cggaacgcta
     4381 cttccgcatc ggacagtacg agagtccgga caacccgct
//
LOCUS       DASRIQ010000383         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_687698, whole genome shotgun sequence.
ACCESSION   DASRIQ010000383 DASRIQ010000000
VERSION     DASRIQ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687698"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFD06_09270"
     CDS             complement(<1..406)
                     /locus_tag="VFD06_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HZN03760.1"
                     /translation="MDPRLPALLIESTAKIALIFFVNLTAIAYLTWLERRVSAWIQDR
                     RGPNRVGPFGLLQPLADGIKFIFKEAITPPQAHVALYLLAPVIIFVPAMAAFAVIPFG
                     PSLEILGRTIPLHVADVDSGVLVILGLSSLGVY"
     gene            complement(406..>2160)
                     /locus_tag="VFD06_09275"
     CDS             complement(406..>2160)
                     /locus_tag="VFD06_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN03761.1"
                     /translation="EVLTMAEPAQPAPAAAPAPVELPPAGPGEVALVVDGRPVVVPKG
                     TTVLQAAERLGIVVPHYCYHPGLSIAGNCRMCLVEVEKTPKLQIGCATTAAQGMIVRT
                     TTPRVTSTRNGIMEFLLINHPLDCPICDQAGECRLQQYAAAYGRGESRFVEEKIHNDK
                     GVPIGPRVVFDGERCIKCTRCIRFCDEVSKTHELGLFERGGHAIIGTFPGKSLDNNYS
                     GNTVDICPVGALTWKPFRFRARVWFLRNVPSVCIGCARGCNVELGTFDHRIRRITPRN
                     NPEVNGWWMCDLGRQTYEPLDGRPRLEQPAARGAIAAGPPGGAAAPAIPVTAGVTPGE
                     ALDRAAALLRETAARHGAASLAAIVSARLTLEDLFMARKALQQAGIGRVVVPPHESGE
                     DDPLLLRRDRTPNARGAEIVGLGRPDAAATARLAADVKAGTVKGLVVVGEDPAGDGLL
                     EAPSLSALEALVVIDAWRTPTAAAAHVALPATGPYEADGVAVNFQGRAQLARRGVPAP
                     GLAEPSWRWLRELGRRFGLAGSWNTASAVFDEAAAAHPPLASLSHRKLGANGHPIVFA
                     EAAPGGGAPAPGTEAREP"
BASE COUNT          296 a          764 c          817 g          283 t
ORIGIN      
        1 cgtagacgcc gagcgacgac agcccgagga tcaccagcac gccgctgtcc acgtccgcca
       61 cgtggagcgg gatcgtccgg ccgaggatct cgagagacgg cccgaagggg atgaccgcga
      121 aggccgccat cgccgggacg aagatgatga ccggcgccag caggtacagg gccacgtgcg
      181 cctgcggcgg ggtgatcgcc tccttgaaga tgaatttgat cccgtcggcg agcggctgca
      241 gcaggccgaa cggcccgacg cggttcggcc cccggcggtc ctggatccag gcggagacgc
      301 gccgctcgag ccaggtcagg taggcgatcg ccgtcaggtt gacgaagaag atcagggcga
      361 tcttcgccgt cgactcgatg aggagcgcgg gaagccgcgg gtccattacg gttcgcgcgc
      421 ctcggtgccg ggcgcgggag cgccgccccc gggggcggcc tccgcgaaga cgatcgggtg
      481 gccgttcgcg cccagcttcc ggtgcgacag ggaggcgagc ggcggatgcg ccgccgcggc
      541 ttcgtcgaag acggcggagg cggtgttcca cgagccggcg aggccaaagc ggcggcccag
      601 ctcgcgcagc caccgccatg acggctccgc caggccgggg gccgggacgc cgcgccgcgc
      661 cagctgggcg cgtccctgga agttcaccgc gacgccgtcc gcctcgtagg gtccggtggc
      721 cggcagcgcg acgtgcgcgg ccgccgcggt cggcgtgcgc caggcgtcga tcaccacgag
      781 cgcctcgagc gccgacaggg acggcgcctc gaggagaccg tctccggccg gatcctcgcc
      841 gacgacgacg agccccttga ccgtgcccgc cttgacgtcg gcggcgaggc gggcggtggc
      901 ggcggcgtcc ggccggccga ggccgacgat ctcggcgccg cgcgcgttcg gcgtccggtc
      961 ccggcgcagc aggaggggat cgtcctcccc cgactcgtgc ggcggcacca cgacgcggcc
     1021 gatgccggcc tgctgcagcg ccttgcgcgc catgaagagg tcctcgagcg tgagacgcgc
     1081 cgagacgatc gccgcgaggc tcgccgcgcc gtgacgcgcc gccgtctcgc gcagaagggc
     1141 cgcggcccgg tcgagcgcct ccccgggggt cacccccgcc gtcacgggga tggcgggcgc
     1201 ggccgcgcct cccggcggac cggccgcgat cgcgccgcgg gccgcgggct gctcgagacg
     1261 cggtcggccg tccagcggct cgtaggtctg ccggccgagg tcgcacatcc accagccgtt
     1321 cacctccggg ttgttccgcg gcgtgatccg gcggatgcga tgatcgaagg tccccagctc
     1381 gacgttgcag ccgcgggcgc agccgatgca gaccgacggc acgttgcgca ggaaccagac
     1441 gcgcgcccgg aagcggaacg gcttccaggt cagcgccccg accgggcaga tgtccaccgt
     1501 gttgccggag tagttgttgt cgagcgactt tccggggaag gtcccgatga tggcgtgtcc
     1561 gccgcgctcg aacagcccca gctcgtgggt cttgctgacc tcgtcgcaga aacggatgca
     1621 gcgggtgcac ttgatgcagc gctcgccgtc gaagacgacg cgcgggccga tcggcacccc
     1681 cttgtcgttg tggatcttct cctcgacgaa gcggctctcc ccgcggccgt aggcggcggc
     1741 gtactgctgc aggcggcact cccccgcctg gtcgcagatg gggcagtcca ggggatggtt
     1801 gatgagcagg aattccatga tcccattgcg cgtcgatgtc actcgtgggg tcgtggtcct
     1861 gacgatcatc ccctgcgccg ccgtggtggc gcagccgatc tgcagcttcg gcgtcttctc
     1921 gacctcgacc aggcacatgc ggcagttgcc ggcgatgctg aggccgggat ggtagcagta
     1981 gtgcggcacg acgatgccga gcctctcggc cgcctgcagg acggtcgtgc ccttcgggac
     2041 gacgaccggc cggccatcga cgacgagcgc cacctcaccg gggccggcgg gcggcagctc
     2101 caccggggcc ggggcggcgg ccggtgcggg ctgggccggc tcggccatcg tcaggacctc
//
LOCUS       DASRIQ010000384         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_161204, whole genome shotgun sequence.
ACCESSION   DASRIQ010000384 DASRIQ010000000
VERSION     DASRIQ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161204"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..883
                     /locus_tag="VFD06_09280"
                     /pseudo
     CDS             125..883
                     /locus_tag="VFD06_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            891..1670
                     /locus_tag="VFD06_09285"
     CDS             891..1670
                     /locus_tag="VFD06_09285"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZN03762.1"
                     /translation="MELRGKVALVTGGGRGIGRGIVDRFLEEGAQVAVVQRKPLDDVL
                     AGNPGVAHVEADLGDPSSIASAVEQAVERFGGVDVLVNNAGIMFERTVSEIRLDEWDL
                     MMAVNLRGPLFLVQAVLPHMRQRGGGSIINIGSIEGLGANPDHAAYCASKAGMHGLTR
                     AVAVDLGADNIRCNAIAPGWIASDLSETYLESQPDPAAARDALNRLHPLGRVGRPTDV
                     GDLAVYLASDRSSFLTGEIVVLDGGRTAQLPTPIHTLGDAS"
     gene            1667..>2054
                     /locus_tag="VFD06_09290"
     CDS             1667..>2054
                     /locus_tag="VFD06_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZN03763.1"
                     /translation="MTIQLERSVRRFDRAVDLHADLAALVDRRTDGFSLEAPFYTSQE
                     VFDLDMAAIWTKHWLFVAAEAELPEPGDYVTVEIGSSSVIIVRDDDEDVRAFHNVCRH
                     RGSRILDDACGSVGNLVCPYHHWTYNV"
BASE COUNT          335 a          709 c          691 g          319 t
ORIGIN      
        1 cgagatcctc agtcacgcac ctcacgacga gcaccttgcg tcttgaccgt cacccggtat
       61 ccggttcaca atccacttag tgggcagcac ccactactca gtgggcagtc tggaagggag
      121 tggcgtgagg ttctcgggga aggttgcgat cgtcacggga ggcgcaggag gcgtcggccg
      181 ggcgctggtg cgcacgctga cgcgcgaagg cgccgccgtg atggtggccg acctgtcggc
      241 ggacggctgc cgcgcgatcg tcgacgagat cgaggtcgcc gctgggtcgg tcgccttcgt
      301 cgccggcgac gtgcgcgatc ggacgtactg cgagcaggtg gtcgcagaga cggtgcagcg
      361 cttcggcggc gtcgacatcc tgttcaacaa cgccgggatc attccgcgag ggacgattct
      421 cgagacgtcc gacgacatgt ggcacgccgc cctcggcgtg aacctcaccg cgatgttcta
      481 cttctgccgc gcggccatcc ccgccatgaa ggaacgcgcg ggaggagcga tcgtcaacac
      541 ctcctccgtg tggggcgtct acccggggcc ggggcacatc gcttactgca ccagcaaggg
      601 cgcggtggcg tccttcacca agagcctggg acgcgaccac gcgcctgacg gcatccgggt
      661 caacgccgtc tgcccgcacg agatcaacac gccgatgctc cgctccgggt tcgagcgccg
      721 gggcctggat ccgcagaaag ccgtcgaaga cctgaacaag accgttccgc tcggtcacat
      781 cgccgagccc gaggagatcg cggacgtcat ggtgttcctc gcctccgacg aggcgcgcta
      841 catcgccggg gagaccgtcg aggtcacggg cgcgaagccg gtgctagctg atggaattgc
      901 gtggaaaggt cgccctggtc accggcggcg gccgcgggat cggccgcggg atcgtcgacc
      961 gcttcctcga agaaggcgcg caggtggccg tcgtccagcg caagccgctc gacgacgtgc
     1021 tggcaggcaa cccgggagtg gcgcacgtcg aagccgacct cggcgacccg tcgtcgatcg
     1081 ccagcgcggt cgagcaggcg gtcgagcggt tcggaggcgt cgacgtcctc gtcaacaacg
     1141 ccggaatcat gttcgagcgc accgtctccg agatccggtt ggacgagtgg gatctcatga
     1201 tggcggtgaa cctccggggg ccgttgttcc tcgtccaggc ggtgctgccg cacatgcggc
     1261 aacgtggcgg cggcagcatc atcaacatcg gctcgatcga agggctcggc gcgaatcccg
     1321 accatgccgc ctactgcgcg tccaaggcag gaatgcacgg gctgacccgc gccgtggccg
     1381 tcgacctcgg cgccgacaac atccggtgca acgccatcgc acccgggtgg atcgcctccg
     1441 atctgagcga gacgtatctg gagtcccagc ccgacccggc cgcggcccgc gacgccctga
     1501 accgcctgca tccgctcggc cgtgtcgggc ggcccaccga cgtcggcgat ctcgctgtgt
     1561 acctggccag tgaccgttcc agctttctga ccggcgaaat cgtcgttctc gacggtggcc
     1621 ggaccgcaca actgccgacg cccatccaca ccctgggaga cgcatcatga ccatccagct
     1681 cgagcgttcg gtccgccggt tcgatcgcgc cgtggacctg cacgccgacc tggcggccct
     1741 cgtcgaccgg cgcaccgacg ggttcagcct ggaagcgccc ttctacacca gccaggaggt
     1801 cttcgacctc gacatggcgg ccatctggac gaagcactgg ttgttcgtgg ccgccgaggc
     1861 ggagctgccc gagcccggcg actacgtcac cgtggagatc ggctcgtcct cggtgatcat
     1921 cgtccgcgac gacgacgagg acgtgcgggc cttccacaac gtctgccggc accgcggatc
     1981 gcggatcctc gacgacgcct gcggcagcgt cggcaacctg gtgtgccctt accaccactg
     2041 gacctacaac gtcg
//
LOCUS       DASRIQ010000385        11505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2774640, whole genome shotgun sequence.
ACCESSION   DASRIQ010000385 DASRIQ010000000
VERSION     DASRIQ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11505
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774640"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..630)
                     /locus_tag="VFD06_09295"
     CDS             complement(28..630)
                     /locus_tag="VFD06_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HZN03764.1"
                     /translation="MLPTRRQKAVLEAIEEHLSETGQTPTLLEIAKRCGLSSVATVHR
                     HLALLQERGLLKRRRSRRRGIELRPAARSSLAVSVPLLGFLAAEKPIEPIPESASVAL
                     PRELVRRPAETFALRVKGDSLRPEQILDGDLLIAERREQWRDGEAVVEILAGGQAVLR
                     PAGRDRGREPGPDRRRDEEGRREDAPRVLGVVTGLLRRYD"
     gene            complement(638..1021)
                     /locus_tag="VFD06_09300"
     CDS             complement(638..1021)
                     /locus_tag="VFD06_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03765.1"
                     /translation="MSIGDSDEAASLTRRDIKKAVKEAVDELLGLGETRMAGRFEGGT
                     LIIRPGKADTQEKVIPVDALFHKITMVRDRLRVLEQRLNTHPKLNDEDRVELQQYVTR
                     CYGSLTSFNVLFRDRDEWFIGSRET"
     gene            complement(1061..2851)
                     /locus_tag="VFD06_09305"
     CDS             complement(1061..2851)
                     /locus_tag="VFD06_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZN03766.1"
                     /translation="MTTATSPNRPTPVASVSPDEARIAPPPAPERPRGRIVYTFLLVL
                     LLAGLAPLGIVARKLITISRESLITSQQEVQLQMATGIARQIESSIDARVAELQRLAE
                     GISGSRAVAARSPEAINAALQRYLGGGLVLLRLTGGFGPAEARREGLDLSAEAAAALK
                     RLEARGGEEVRTGEPILWAGGGERHALLPIAVAAGPPEAGRTLLGLVDLAVVWAPVSS
                     AGRSAYTVYLLDRNANLLASLDESGTLQRDDLRSYGVVQEFLQNGGRSATTTEFRAVR
                     EGKPIEYLASVDGTAQGWGIFVQLEKRKAYAAVEQMVRTTLVWAGLAAVGALVLAYLL
                     AAMVTRPIHALAAGTEAFARGALDHRVEVTSRNELGALAATFNGMARQMQEYIQRLKV
                     AAQVNNELFMGTIRALADAIDEKDPYTRGHSERVNQYAVLLAKQLGLEKKELRQVHIA
                     SLFHDIGKIGIEDKILRKPAMLTDQEYAVMKQHPEKGAQMLSKIKAMRDIIPGMRFHH
                     ERWDGTGYPLGLKGEQIPMAARIVAVADAFDAMTTNRPYQKAMPFDKAIERLLELSER
                     VYDRKVVIAFAEAFRAGLFKEPQLAEVEEQ"
     gene            complement(2955..3950)
                     /gene="nadA"
                     /locus_tag="VFD06_09310"
     CDS             complement(2955..3950)
                     /gene="nadA"
                     /locus_tag="VFD06_09310"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZN03767.1"
                     /translation="MAAEAATAEFVPEIWRPADPTATAALVQEVKALARERDAVLLAH
                     NYQLPEVQEAADFVGDSLGLALQAQKTGASTIVFCGVHFMAESARVLNPDKTVLLPHL
                     AAGCALADSISAESLLEWKARYPGHAVVTYINSSAEVKALSDICCTSANAVSVVRSLP
                     DTNILFTPDRNLGRWVAQQVPEKNIAVYDGACPTHDVLRSASVDKVKKQRPEAVVIAH
                     PECRDDVLAMADEICSTSGMIDAVARHPEARTFIVATEWALIHQLKKRYPGREFVPAD
                     GCIGCRLHCPYMKMIDLPAVRRALLEGVFPIEVDPDDAAGARRALERMMAVPRDR"
     gene            complement(3940..4182)
                     /locus_tag="VFD06_09315"
     CDS             complement(3940..4182)
                     /locus_tag="VFD06_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03768.1"
                     /translation="MAAKVTDPAGAAAGKRRVRTFTATRQDLEMLETVARYHGFSKSG
                     TITSLIRREFWRLFPAGTRGIRPERGARVLGRGYGG"
     gene            complement(4289..5545)
                     /locus_tag="VFD06_09320"
     CDS             complement(4289..5545)
                     /locus_tag="VFD06_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZN03769.1"
                     /translation="MNRPVLLIIDNGSVTRLAHKEAIEACCPREAEVLTAASAEEALG
                     TLYTLRDQGRTIEMVIVSQGLPGIPGNRLLEIVSTQFPAVCRILLSDTPSLDEAIYAF
                     NNSGVDRYIPLPWEREDIKFTISSLLRQREMNRLNERLLSDLQVRNQELVVAFRNLDE
                     AKKENERSYIQTVQSLAVALEAKDRYTAGHSQRVARFATLIARAMGLAKDQVEEVAQV
                     ALLHDIGKIGMLDSILNKPSNLTAEERELVKSHPVIGAQILAPVKTFERHVVGIRYHH
                     EMFDGTGYPDKLKGEEIPVTARIVSLADAFDAMTSTRPYRIGLPLAFAMQEMIKMRGR
                     QFCPMAVDAFVRVLQSTAAGDVPGLVGGGAESAPANAATPAAPAATAATTPLAPTSAP
                     TPDPVEPDRRDGENAAAPPDTKRNAA"
     gene            5633..6949
                     /locus_tag="VFD06_09325"
     CDS             5633..6949
                     /locus_tag="VFD06_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HZN03770.1"
                     /translation="MDALYGAALRLAWVASIPYQIVMEFLSGRPAARRRERIGRAADL
                     ARIPVGGLWVHAVSFGEVRLAHAVIAALKARLGAVPILLTTSTGTGRALAERQRRDAG
                     PGAADAIAAPPLDLPGPIGRLLDAARPRALILIETELWPNLLRLLPARGIPVIVVNGR
                     LSPRAFARVLKVRSLFAPGVAAIRCVAAQTEADARRFVALGAPAERVRVTGNIKFDLP
                     IPAPGPDAPRRRMGLGDDTPMFVAGSTARGEAEPVARAFAHLRSAVPGAVLVIAPRHP
                     EDVDATRRVLEQAGRRVALWSGLEAGAPRAAAVDAVVVDAMGVLPSLYSCATVAFVGG
                     SLVPRGGQNLLEPAAAGVPVLFGPWVDTVRFAADALLASGGGFQVSDAASLGDEVLRL
                     TTDAAARAQAGDRARETVLRNRGPLPRTIDLVVAALEAPAARGAAG"
     gene            6946..8070
                     /gene="lpxK"
                     /locus_tag="VFD06_09330"
     CDS             6946..8070
                     /gene="lpxK"
                     /locus_tag="VFD06_09330"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZN03771.1"
                     /translation="MTPPFRQADRDPWDLPPAARAALLLPSFLYAGAVWGRNRLYDAG
                     LLPSARLPFPVVAIGNLTVGGSGKTPIASYLAGALLARGVAVAIASRGYGRTGAAPQL
                     VSDGRRLLADARAAGDEPVLLARTHPGAAVAVAADRAAAARLLPDLPRPRALLLDDAF
                     QHRAVRRDVDLLLVDAKAPFGNGRILPLGPLREPVSGLRRADAVVVTRGDGTCPAALR
                     EALERHHPNAPIFHASIVPSRLEGVAGESLSLDRLRGRPVFAFSGIAHPARFEADLLG
                     LGAVLAGTRRFRDHHAYGAGDLAEIVTEARAAAAEALVTTAKDRVRLDGPLPAGAPPL
                     LALGIEVSFPSGADLADFVHDRLRRLPGHDGGAPGTQSAA"
     gene            8067..9092
                     /locus_tag="VFD06_09335"
     CDS             8067..9092
                     /locus_tag="VFD06_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN03772.1"
                     /translation="MSRASSEPSRATVGQWIGYAVGRSVFGLLAILPLRAARAAGAGL
                     GRLFFLVSPRHRGIARRNLASALGDTLGPAERERVVRACFAHLGRIVADATHFPEHLR
                     RPTEAVAVYEGVEHLKAAAAQGKGVLVFSGHFGHWELIAFLQHRLGLPMTMVVSPLEN
                     PLYDRFIARLRGLGGNRILAKRHAARPILKALANREAIAILIDQNVRGEGGLFIDFFG
                     RAASSTPALATLALRSGAPIVPVFSWFLPDGRLQISYRPALEPHRLGSIEEDVRDLMC
                     RSTALLEEEIRRRPDHWLWMHDRWRTRPRPGQVAPASRPAPGAPVPLPAAQKPGGAIH
                     HRSAARP"
     gene            9089..9676
                     /locus_tag="VFD06_09340"
     CDS             9089..9676
                     /locus_tag="VFD06_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN03773.1"
                     /translation="MSHRAIFMDRDGTVCEEMGYVNHLSRSRLLPRSLEAIRLANESG
                     YLVVVATNQSGVARGLFAEELVRAVHRQLADRAAASGARIDALYFCPHHPREGAPPWR
                     LDCDCRKPKPGMLTRAAREHHIDLGRSWMVGDGFPDIEAGTAAGTRVVQVLTGYGRGL
                     VEHHQDEFRSRPEHTAEDLLDAVHYIVACDREPRT"
     gene            9673..10725
                     /locus_tag="VFD06_09345"
     CDS             9673..10725
                     /locus_tag="VFD06_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZN03774.1"
                     /translation="MKEAKNRGGRGPERQRLLALVDAMAGTPVLAVADLVLDVFEHGR
                     IGRISREAPVLILDHVRTDRLPGGGANVAANLAALGARTEVVGRAGSDAAGDDLLAAL
                     ERLGAGVRGVWRDPRYRTPEKRRILAGSAHSVQQQIVRLDSGGGTPAPPEALLERLRP
                     ALPAAKGVVLSDYSLGLFHDATIGTVLVASREAKKPVFVDSRSQLGRFHGLAAATPNL
                     QEAEAALGGPIDGDPDRLLAAAEAIRERLEADAVVVTLGSLGMAVAGRGGARARLDVY
                     GTDEVADVTGAGDTVIAALSLAVLAGADPIEAALLANYAGGIVVMKRGTATVSPEELR
                     HAVLHDPRFEERLAGR"
     gene            10722..11216
                     /locus_tag="VFD06_09350"
     CDS             10722..11216
                     /locus_tag="VFD06_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HZN03775.1"
                     /translation="MSAAPVLGLREAERRLRRWRRRPGRARRGGRRVVLANGLFDLLH
                     VGHLRYLRGARRLGDFLVVAVNDDRSARALRGPGRPIVPARDRARLVAAVRGVDLVVI
                     FGGRTVGTVLRRLRPEVHCKGTDYTPATVPERDIVRGYGGVVRTAGDAKRHATTDLVA
                     RLAG"
     gene            complement(11221..>11505)
                     /locus_tag="VFD06_09355"
     CDS             complement(11221..>11505)
                     /locus_tag="VFD06_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HZN03776.1"
                     /translation="IADKDVVKKLFATLGPRFATRPGGYTRTMKLGPRQGDGAEMAFV
                     ELVGSEGLFKKQREERQAKKDRRAAAMRKKAEDSTPPEDKGEGSSGAAAG"
BASE COUNT         1435 a         4217 c         4124 g         1729 t
ORIGIN      
        1 gatggggatg gcggaacggt cgccgggtca gtcgtagcgc cgcaggagcc ccgtgaccac
       61 gccgaggacc cggggggcat cttcacggcg cccctcctcg tcccggcggc ggtcgggtcc
      121 cggctccctg ccccgatcgc gcccggccgg gcgcagcacc gcttgcccac ctgcgaggat
      181 ttcgacgacc gcctccccgt cgcgccactg ctcccgccgc tccgcgatga gcaggtcgcc
      241 gtcgaggatc tgctcgggac gcagggaatc ccccttgacc cgcagggcga aggtctccgc
      301 gggacggcgc accagttccc gcggcagcgc cacgctcgcg ctctcgggga tcggctcgat
      361 cggcttctcg gccgccagaa accccaggag cggcacgctg accgccaggc tgcttcgcgc
      421 cgccgggcgc agctcgatgc cgcgccggcg gctgcggcga cgcttgagga ggccacgctc
      481 ctgcaggaga gccaggtgac ggtgcaccgt ggccaccgac gagaggccgc agcgcttcgc
      541 gatttcgagg agcgtcggag tctgcccggt ctccgacagg tgctcctcga tcgcctcgag
      601 caccgccttc tggcggcggg tgggaagcat caggctccta ggtctcgcgc gatccgatga
      661 accattcgtc gcgatcgcgg aacaggacgt tgaacgaggt cagcgatccg tagcagcggg
      721 tgacgtactg ctgcagctcc acccggtctt cgtcgttcag cttgggatgg gtgttgaggc
      781 gctgctccag gacccgcagg cggtcgcgca ccatggtgat cttgtggaac agggcgtcga
      841 ccgggatgac cttctcctgc gtgtcggcct tgccgggccg gatgatcagc gtccccccct
      901 cgaagcgccc ggccatgcgg gtctccccga ggccgaggag ctcgtccacc gcctccttga
      961 ccgctttctt gatgtcgcgg cgcgtcaggc tcgccgcctc gtcgctgtct ccgatgctca
     1021 tcgcgtcctc cgctgcggcg attccgcccc cctactgcga ctactgctcc tcgacctcgg
     1081 ccagctgcgg ctctttgaag aggccggcgc ggaacgcctc cgcgaaggca atcaccacct
     1141 tccgatcgta gacccgctcc gacagctcca ggagccgctc gatcgccttg tcgaacggca
     1201 tcgccttctg gtacggccgg ttggtcgtca tcgcgtcgaa ggcgtcggcc accgccacga
     1261 tgcgggccgc catcgggatc tgctccccct tgagaccgag cgggtagccg gtgccgtccc
     1321 agcgctcgtg gtggaagcgc atccccggga tgatgtcgcg catcgccttg atcttcgaca
     1381 gcatctgcgc ccccttctcg ggatgctgct tcatgacggc gtattcctgg tcggtcagca
     1441 tcgccggctt ccggaggatc ttgtcctcga tgccgatctt cccgatgtcg tggaacagcg
     1501 acgcgatgtg gacctgccgg agctccttct tttcgaggcc gagctgcttc gcgaggagga
     1561 cggcgtactg gttcacccgc tcggagtggc cgcgcgtgta ggggtccttc tcgtcgatgg
     1621 cgtcggcgag ggcgcggatc gtccccatga acagctcgtt gttcacctgc gccgccacct
     1681 tgaggcgctg gatgtactcc tgcatctgcc gggccatgcc gttgaaggtg gcggcgagcg
     1741 ccccgagctc gttgcggctc gtgacctcga cgcggtgatc gagggcgccg cgcgcgaacg
     1801 cctcggtgcc ggcggccagc gcgtggatcg gccgggtgac catcgccgcc agcaggtacg
     1861 cgagcacgag cgctccgacg gcggcgaggc cggcccagac caaggtggtg cgcaccatct
     1921 gctcgacggc ggcgtacgcc ttgcgcttct ccagctggac gaagatcccc cagccctgcg
     1981 ccgtgccgtc caccgaggcc aggtattcga tcggttttcc ctcccgcacg gcgcggaatt
     2041 cggtggtggt ggcggaacgc ccgccgttct ggaggaactc ctgcaccaca ccgtacgagc
     2101 gcagatcgtc ccgctggagc gtccccgatt cgtcgagcga ggcgaggagg ttggcgttgc
     2161 ggtcgagcag gtagaccgtg taggcggagc ggccggccga gctgacgggg gcccagacca
     2221 ccgcgagatc cacgagcccg agaagggtgc gccccgcctc cggtggaccg gccgcgacgg
     2281 cgatcggcag gagcgcatgc cgctcccctc cgcccgccca gaggatcggc tcgccggtgc
     2341 gcacctcctc gccaccgcgc gcctccaggc gcttgagggc cgcggccgcc tcggcggaaa
     2401 gatcgagccc ctcccggcgc gcctcggccg gcccaaaccc gcccgtcagg cggagtagca
     2461 ccagaccgcc gccgaggtag cgctgcagag ccgcgttgat cgcctcgggg gatcgcgcag
     2521 cgacggcgcg cgagccggag atcccctcgg cgagccgctg cagctcggcg acccgtgcat
     2581 cgatcgacga ctcgatctgg cgggcgatgc cggtggccat ctgcagctgc acctcctgct
     2641 gcgaggtgat gagcgattcg cggctgatgg tgatgagttt gcgggccacg atgccgagcg
     2701 gcgccagtcc cgccaggagc agcaccagca ggaaggtgta gacgatgcgc ccgcgcggcc
     2761 gctccggggc gggaggcggg gcgatgcgcg cctcgtcggg agagacggag gcgacgggag
     2821 tcggacgatt cgggctggtc gctgtcgtca cagagaagag tcccggaacg aatccggaac
     2881 gcggggggat tctaggacgc cgtcgcgcgg ggcgtcaacg catccgccga tggcccccgg
     2941 gtcccgagga gcgatcagcg gtcccgcggc accgccatca tgcgttcgag ggcccgccgg
     3001 gctcccgccg cgtcgtcggg gtccacctcg atcgggaaga ccccctctag aagcgcccgg
     3061 cgcaccgccg gcaggtcgat catcttcatg taggggcagt gcagccggca gccgatgcac
     3121 ccgtcggccg ggacgaactc gcggcccggg tagcgtttct tcagctggtg gatgagggcc
     3181 cactcggtcg cgacgatgaa cgtgcgggcc tccgggtgcc gtgccaccgc gtcgatcatc
     3241 ccggacgtcg agcagatctc gtccgccatg gccaggacgt catcacggca ctccgggtgg
     3301 gcgatcacca cggcctcggg ccgctgcttc ttcaccttgt ccacgctcgc ggagcgcagg
     3361 acgtcgtgcg tcgggcaggc gccgtcgtac accgcgatgt tcttctccgg cacctgctgc
     3421 gccacccagc gcccgaggtt gcggtcgggg gtgaacagga tgttcgtgtc gggaaggctg
     3481 cgcacgactg acaccgcgtt ggccgaggtg cagcagatgt cggacagcgc cttcacctcg
     3541 gcgctgctgt tgatgtacgt gaccaccgcg tgccccgggt agcgcgcctt ccactccagg
     3601 agcgactcgg cgctgatcga atcggcgagg gcgcagccgg cggcgaggtg cggcaggagc
     3661 accgtcttgt cggggttgag gacccgggcc gactccgcca tgaagtgcac gccgcagaag
     3721 acgatggtgg aggcgccggt tttctgcgcc tgcagcgcca ggccgagcga gtcgccgacg
     3781 aagtcggccg cttcctggac ctcggggagc tggtagttgt gcgccagcag caccgcgtcc
     3841 cgctcgcgcg ccagggcttt gacctcctgc acgagcgccg cggtcgcggt cggatcggcc
     3901 ggacgccaga tctccgggac gaactcggcg gtcgcggcct cagccgccat atcccctccc
     3961 cagcacgcgc gccccgcgct ccggccggat gccgcgggtc ccggccggga acagccgcca
     4021 gaactcgcgc cggatgaggc tcgtgatggt gccgctcttg gagaagccgt ggtagcgcgc
     4081 caccgtttcg agcatctcga gatcctgccg cgtcgcagtg aaggtgcgca cgcggcgctt
     4141 ccctgccgcc gcccccgcgg ggtcggtcac cttcgcggcc atctttgcgc tccgtttccg
     4201 tatttggttc atatatcaat catgtatatc ttcgccacga gattatcgcg ccacccggtc
     4261 gatgtcaagc cggtcgggac gcgccggatc aggcagcgtt tcgcttggtg tcgggaggag
     4321 cagcggcgtt ttcgccgtcg cgccggtccg gctcgaccgg gtcgggggtc ggggccgagg
     4381 tcggcgcgag gggggtcgtg gctgcggtcg ccgcaggcgc tgcaggtgtc gcggcgttcg
     4441 ccggggctga ctccgctccc ccgccgacga ggcccggcac gtcgccggcg gcggtggact
     4501 gcagcacccg gacgaaggcg tccaccgcca tcgggcagaa ctggcggccg cgcatcttga
     4561 tcatctcctg catggcgaag gccagcggca ggccgatgcg gtaggggcgc gtcgaggtca
     4621 tggcgtcgaa ggcgtcggcg agcgacacga tgcgggcggt gacgggaatc tcctccccct
     4681 tcagcttgtc ggggtagccc gtgccgtcga acatctcgtg gtggtagcgg atgccgacga
     4741 cgtggcgctc gaaggtcttc accggcgcca ggatctgcgc gccgatgacc ggatgcgact
     4801 tgaccagctc acgctcctcg gcggtcaggt tggacggctt gttgaggatg ctgtcgagca
     4861 tcccgatctt gccgatgtcg tgcaggaggg ccacctgcgc cacctcctcg acctggtcct
     4921 tggccagccc catcgctcgc gcgatgagcg tcgcgaagcg cgccacccgc tgcgagtgcc
     4981 cggcggtgta gcggtccttc gcttcgagcg ccaccgccag cgactgcacg gtctggatgt
     5041 acgagcgctc gttctccttt ttcgcctcgt cgaggttgcg gaacgccacc accagctcct
     5101 ggttgcgcac ctggaggtcg gagagcagcc gctcgttgag ccggttcatc tcgcgctggc
     5161 gcaggaggga cgagatggtg aacttgatgt cctccctctc ccacggcaac ggaatgtagc
     5221 gatcgacccc cgagttgttg aaggcgtaga tcgcctcgtc gagcgacggc gtgtcgctga
     5281 gcaggatgcg gcagaccgcc gggaactgcg tcgagacgat ctcgaggagg cggttcccgg
     5341 gaatgccggg aagcccctgc gacacgatga ccatctcgat ggtccgtccc tgatcgcgga
     5401 gcgtgtagag cgttccgagc gcctcctcgg ccgaggcggc cgtcagcact tcggcctcgc
     5461 gcgggcagca ggcctcgatc gcctccttgt gggccaggcg tgtgaccgag ccgttgtcga
     5521 tgatcaggag gacggggcgg ttcatgccga accaatatag gaaggggctt cggaggacgc
     5581 cagatgtcgg tccgttgacg ctccccggtc gatgccatag actcgcgccc cgatggacgc
     5641 cctctacggg gcggccctgc ggctggcctg ggtcgcgtcg atcccttacc agatcgtcat
     5701 ggagttcctg tccggccggc ccgcggcccg gcggcgcgag cggattggcc gggcggcgga
     5761 tctcgcccgg atccccgtcg gcggcctgtg ggtgcacgcg gtgtcgttcg gcgaggtgcg
     5821 gctggcgcac gccgtcatcg ccgcactcaa ggcacgcctc ggcgccgtgc cgatcctcct
     5881 gaccacctcg accgggacgg ggcgcgccct cgcggagcgg cagcggcgcg acgcgggccc
     5941 cggcgccgcc gacgcgatcg ccgcgccgcc gctcgatctg cccggtccga tcggccgcct
     6001 gctcgacgcc gcgcgcccgc gggccctgat cctgatcgag accgagctgt ggccgaacct
     6061 cctgcgcctc ctgccggcgc gcggcattcc cgtgattgtc gtcaacggcc ggctgtcgcc
     6121 acgcgccttc gcccgcgtcc tcaaggtgcg gagcctcttc gcccccggcg tcgcggcaat
     6181 ccgctgcgtg gcggcgcaga cggaggcgga tgcgcgccgc ttcgtcgcgc tcggcgcccc
     6241 ggcggagcgg gtgcgggtga ccggcaacat caagttcgac ctgccgatcc cggcccccgg
     6301 ccccgacgca cctcgccgcc ggatgggact gggcgacgac acgccgatgt tcgtggccgg
     6361 cagcaccgcc cgcggcgaag cggagccggt cgcccgcgcg ttcgcgcatc tgcgctccgc
     6421 cgtccccggc gccgtcctgg tgatcgcccc gcgccacccc gaggacgtcg acgcgacccg
     6481 ccgggtcctc gaacaggccg gccggcgcgt ggcgctctgg agcggcctcg aggccggtgc
     6541 gccccgcgcc gccgcggtgg acgccgtggt cgtcgacgcg atgggggtgc ttccttcgct
     6601 ctattcctgc gccacggtgg ccttcgtcgg ggggagcctc gtgccgcgcg gtggacagaa
     6661 cctgctcgag ccggcggccg ccggcgtgcc ggtcctgttc ggaccatggg tggacaccgt
     6721 ccgcttcgcg gccgacgcgc tgctcgcctc cgggggcggc ttccaggtga gcgatgccgc
     6781 atccctcggg gacgaagtgc tgaggctcac gaccgacgcg gcggcgcggg cgcaggcggg
     6841 cgacagggcc cgcgagacgg tcctccggaa ccgggggccg ctcccccgca ccatcgacct
     6901 cgtcgtggcc gcgctcgaag cgcccgcggc gcgcggcgcc gccggatgac gcctccgttc
     6961 cggcaggcgg atcgcgatcc ctgggacctc ccgccggcgg cgcgggcggc gcttctcctc
     7021 ccttcctttc tttatgccgg cgcggtgtgg gggaggaacc gcctgtacga cgcggggctg
     7081 ctgccatcgg cgcggctccc ctttccggtc gtggcgatcg gcaacctgac ggtcggaggg
     7141 tcggggaaga cgccgatcgc gtcctacctc gcgggggcgc tcctggcccg cggcgtcgcc
     7201 gtcgccatcg ccagccgcgg ttacggccgg accggcgccg cgccgcagct cgtctccgac
     7261 ggccgccgcc tgctcgccga cgcgcgcgcc gccggggacg agccggtcct gctggcccgg
     7321 acccacccgg gcgccgccgt cgcggtcgcg gccgaccgcg ccgccgccgc gcgcctcctg
     7381 cccgacctgc cgcggccgcg ggcgctcctc ctcgacgacg cgttccagca ccgcgccgtg
     7441 cggcgcgacg tcgacctgct cctcgtcgac gccaaggctc cgttcggcaa tggtagaatc
     7501 ctccccctcg gcccgttgcg cgagccggtg tcagggctgc ggcgggccga tgccgtcgtc
     7561 gtgacccgcg gcgacggcac atgcccggcg gccctccgcg aggccctgga gcgacaccat
     7621 ccgaacgcgc cgatcttcca cgcctccatc gtcccgagcc gcctcgaagg ggtggccggc
     7681 gagtccctgt ccctggaccg cctgcgcggg cgtccggttt tcgcgttctc cggcatcgcg
     7741 cacccggcgc gcttcgaggc cgaccttctc gggctcggag ccgtcctcgc ggggacgcgg
     7801 cgcttccgcg atcatcacgc ctacggcgcg ggggatctcg cggagatcgt tacggaagcc
     7861 cgggccgcgg cggccgaggc tctggtgacg accgcgaagg atcgggtccg cctggacggt
     7921 ccgctcccgg ccggggcgcc gccgcttctg gccctgggga tcgaggtgtc gtttccctcc
     7981 ggagccgacc tcgcggattt cgtgcacgat cgcctgcggc gcctgcccgg acacgacggc
     8041 ggcgccccgg gcacgcagag cgccgcgtga gccgggcgtc ctcggaaccg tcgcgcgcga
     8101 ccgtcgggca gtggatcggc tatgccgtgg ggcgatcggt gttcggtctc ctggcgatcc
     8161 ttccgctgcg cgcggcgcga gccgccggcg ccggcctggg gcggctcttc ttcctcgtct
     8221 cgccgcgtca ccgcggcatc gcccggcgca acctcgcgtc ggccctcggc gacacgctcg
     8281 ggccggcgga gagggagagg gtggtgcgcg cctgcttcgc gcatctcggc cgcatcgtcg
     8341 ccgacgcgac gcacttcccc gagcacctgc gccgcccgac cgaggcggtc gcagtctacg
     8401 aaggggtcga acacctgaag gccgccgcgg cgcaggggaa aggggtcctg gtcttctccg
     8461 ggcacttcgg ccactgggag ctgatcgcct tcctgcagca ccgcctcggc ctgccgatga
     8521 ccatggtcgt gagcccgctc gagaacccgc tctacgatcg cttcatcgcg cggctgcgcg
     8581 gcctcggcgg caatcgcatc ctcgcgaagc ggcacgccgc ccgaccgatc ctcaaggccc
     8641 tcgcgaaccg ggaggcgatc gccatcctca tcgatcagaa cgtgcgcggc gagggggggc
     8701 tgttcatcga tttcttcgga cgcgccgcgt cgagcacccc ggcgctcgcc acgctggcgc
     8761 ttcgcagcgg cgccccgatc gtcccggtct tctcctggtt ccttcccgac gggaggctcc
     8821 agatctccta ccggccggcg ctcgagccgc accgcctcgg ctcgatcgaa gaggacgttc
     8881 gcgacctgat gtgccgatcg accgccctcc tcgaggagga aatccgccgc cgccccgacc
     8941 actggctgtg gatgcacgat cgctggcgca cgcggccgcg gcccgggcag gtcgcccccg
     9001 cctcccggcc cgccccgggc gcgccggtcc cccttccggc cgcgcagaag cccggcgggg
     9061 cgattcacca ccggagcgcg gcacggccat gagccaccgg gcgatcttca tggaccggga
     9121 cggcaccgtc tgcgaggaga tggggtacgt caaccacctg tcgcgcagcc gcctcctgcc
     9181 gcgcagcctc gaggcgatcc gcctggcgaa cgagtccggc tacctcgtcg tcgtcgccac
     9241 caaccagtcg ggcgtggcgc gcgggctctt cgccgaggag ctcgtgcggg cggtgcaccg
     9301 gcagctcgcg gatcgggccg cggcctcggg agcccggatc gacgccctgt acttctgccc
     9361 gcaccacccg cgcgagggtg cgccgccgtg gcggctcgac tgcgactgcc gcaagccgaa
     9421 gcccggcatg ctcacccggg cggcgcgcga gcaccacatc gatctcggcc gctcgtggat
     9481 ggtcggcgac ggcttccccg acatcgaggc cgggaccgcc gccggcacgc gcgtcgtgca
     9541 ggtgctgacc ggctacggcc gaggcctcgt ggagcaccac caggacgagt tccgctcccg
     9601 cccggagcac accgcggaag atcttctgga cgcggtccac tacatcgtcg cgtgcgaccg
     9661 ggagccacgg acgtgaagga ggcgaagaac cgcggcggac ggggtccgga gcgccagagg
     9721 ctcctcgccc tcgtcgacgc catggcgggg acgccggtgc tggcggtcgc cgacctcgtc
     9781 ctcgacgtct tcgagcacgg tcgcatcggc cgcatctcgc gcgaggcgcc ggtgctcatc
     9841 ctcgatcacg tccgcaccga tcggcttccg ggcggcgggg cgaacgttgc ggccaacctg
     9901 gcggcgctcg gggccaggac cgaggtcgtc ggccgcgccg ggagcgacgc cgccggggac
     9961 gaccttctcg cggcgctcga gcggttgggg gcgggcgtgc ggggggtgtg gcgcgacccg
    10021 cgctaccgca cgcccgagaa gcgccgcatc ctcgccggca gcgcccattc ggttcagcag
    10081 cagatcgtcc ggctcgattc cggaggcggg acccccgccc caccggaggc cctgctcgag
    10141 cggctccggc ccgccctgcc cgcggcgaaa ggggtggtgc tgtcggacta ctcgctcggg
    10201 ctcttccacg acgcgacgat cgggacggtc ctggtcgcgt cgcgcgaggc gaagaagccg
    10261 gtcttcgtcg attcccgatc gcagctcggg cggttccacg ggctggcggc cgccaccccg
    10321 aacctgcagg aggcggaggc ggccctcggc ggaccgatcg acggcgatcc ggaccgactg
    10381 ctggccgcgg ccgaggccat ccgcgagcgg ctggaggccg acgcggtcgt cgtcaccctc
    10441 gggagcctgg ggatggcggt cgccggccgc ggcggcgccc gcgcacgcct cgacgtctac
    10501 ggcaccgacg aggtggcgga cgtcaccggc gccggggaca cggtgatcgc ggccctgtcg
    10561 ctcgccgtgc tcgccggcgc cgacccgatc gaggcggcgc tgctcgcgaa ctacgccggc
    10621 gggatcgtgg tgatgaagcg cgggacggcg accgtctcgc cggaggagct gcggcacgcg
    10681 gtgctccacg acccgcggtt cgaggagcgg ctcgccggac ggtgagcgcc gccccggttc
    10741 tcgggctccg ggaggcggag cgccggctgc gccgctggcg ccgcagaccc ggtcgcgccc
    10801 gccgtggcgg ccggcgcgtc gtcctggcga acggcctctt cgacctcctg cacgtcggcc
    10861 acctgcgcta cctgcgcggc gcgcgccggc tcggagactt cctggtggtg gcggtcaacg
    10921 acgatcgctc cgcccgcgcg ctgcgcggcc cgggacgtcc gatcgtcccg gcgcgggacc
    10981 gggcgcgcct cgtcgccgcg gtgcgcggcg tcgatctcgt ggtgatcttc ggcgggcgca
    11041 cggtcgggac ggtgctccgg cgcctccgtc ccgaggtgca ctgcaagggg actgactaca
    11101 ccccggccac ggtccccgaa cgggacatcg tgcggggcta cggcggcgtc gtgcgaacgg
    11161 ccggggacgc gaagcggcac gccacgaccg atctcgtggc gcggctggcc ggttaggagc
    11221 ctagccggcg gccgctccgg aggagccttc gcccttgtcc tcgggcggcg tcgagtcctc
    11281 cgccttcttg cgcatcgcgg cggcgcggcg gtctttcttc gcctggcgct cctcgcgctg
    11341 cttcttgaac agcccttcgg agcccacgag ctcgacgaac gccatctccg cgccgtcccc
    11401 ctgccgcggt ccgagcttca tcgtccgggt gtaccccccc gggcgggtgg cgaaacgcgg
    11461 tccgagcgtg gcgaacagct tcttgacgac gtccttgtcg gcgat
//
LOCUS       DASRIQ010000386        12512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_936699, whole genome shotgun sequence.
ACCESSION   DASRIQ010000386 DASRIQ010000000
VERSION     DASRIQ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936699"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFD06_09360"
     CDS             complement(<1..895)
                     /locus_tag="VFD06_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZN03777.1"
                     /translation="MDPITHTLLGAGLANGLFRERVGRAALPTLLLASNLPDLDGIVH
                     LTSDPYAVLMRRTFGHSLFLLPFWALALAWILGRRYREVPLRTLYGMTLAGVAGHLLF
                     DLINSFGVVLFWPLSDVRPELAWVFIIDFVLTGLLALPLALALLPRLRPRLRSLSRGA
                     LAAVAIYLVACGAARWRAETLLEASAPSGADFAYVFPEPLGPHRWRGVVRSPGRYDLF
                     LIRPLVGQVEPRGTVRNDLDDPRVAAARQTTDGRRLERFFKAPVWQVEASAAVSAWDL
                     RFKPLVVDRRAVFRYTLVPPGG"
     gene            complement(935..1501)
                     /gene="pyrE"
                     /locus_tag="VFD06_09365"
     CDS             complement(935..1501)
                     /gene="pyrE"
                     /locus_tag="VFD06_09365"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZN03778.1"
                     /translation="MDPRARLKALIEQKCILTGEFTLASGQKSSVYFDCKRATLDPEG
                     LHLLGTLLLEQIDRLEQQDGRPIDAIGGPTIGADPVVGHVAGRSWERHQAGKGHPVRA
                     FLVRSASKDHGTERLIENDIPMGSRVLVFEDVVTTAKSTKAAIDKIEAAGLDIAAVVA
                     VVDREQGGADALARWRFQPLFRKSEFGL"
     gene            1574..3301
                     /locus_tag="VFD06_09370"
     CDS             1574..3301
                     /locus_tag="VFD06_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HZN03779.1"
                     /translation="MSSEPDLTDSRSTLMQDPPRRRPVSLSAYLVARLLRLRPNHGPR
                     PMGQVKRDQLDAPRWALTLGILLFLILFLTLIILFFVAAPPVSAASDGVNFDKPTKDW
                     YQGPVRYIITKVETKAYKALETESERLTFIDWFWQRRDIEPSTPQNEFRDRYEQRVFE
                     AQRKFISSATPGWKTDMGKIYILVGPPDEINSDVVAQTHRGIITWVYRRPPFPDMQPN
                     TVIGFARDPSGEFRLSINPTIDSDVARGLKFSRTMITADQTLIRAGQVDPALLAAGAT
                     LSASQIETNMTYGRMQMLPPHEEELFKSFVSSRESYGSTIPMETRFDFYRAGDDTYTT
                     VTVGIRSTAVQYRTRGGKEVPDVGVFGKLVSKENPELSYPLSSESGFADSPENASAGI
                     GDLLIFQAVGAFKPGLYRAILGVEDRVSNKLSSLVKEIEVPDLTGTALRLSSITVAGS
                     MEPTEYATSTAKPFQIGKFKIVARPDAAFSKSDELDVYFQVYGPASDPATGKPKMDVL
                     YSFRRRAEDGTYQDVGTYRVADSPAQVQGYAVPLEKWPEGTYTLTVTIVDKVAKAQAT
                     GDAIFTIRP"
     gene            complement(3311..3985)
                     /gene="rdgB"
                     /locus_tag="VFD06_09375"
     CDS             complement(3311..3985)
                     /gene="rdgB"
                     /locus_tag="VFD06_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZN03780.1"
                     /translation="MTRASPSGGAEGDAEPALLIASFNPGKARELQALLEAFGRSAVT
                     LADLGIREPYEETGNTYEENGLGKARHYARLSGKVTIADDSGLEVEALGGAPGPLSAR
                     YGGPRLDDAGRNRALLAALADVPDDRRGARYVAVAVVARPDGEARSFRGECPGRIATT
                     PRGEKGFGYDPLFFYPPYGATLAEVGDARKHAVSHRGAAFRLLTEFLGSGEGEQFLRG
                     AGVAAR"
     gene            complement(3982..4728)
                     /gene="rph"
                     /locus_tag="VFD06_09380"
     CDS             complement(3982..4728)
                     /gene="rph"
                     /locus_tag="VFD06_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZN03781.1"
                     /translation="MQRLDGRDERQGRPIEILTGVNMHAEGSALIKVGQTHVLCMASV
                     EDRVPAFMRDTGQGWVTAEYGMLPRATNERTSRESTKGRPSGRTQEIQRLIGRSLRAV
                     VDAKALGQRTVWIDCDVLQADGGTRCAAISGAYVALALALAKLRAKNLLGGPVLRGLV
                     SATSVGIVGGLACLDLSYVEDSQADVDMNVVRTDAGRFVEVQGTAEKHPFSLEEMQRL
                     LDLAVLGTDRMFEVQREVLHDVLPPLLPRT"
     gene            complement(4761..5552)
                     /gene="murI"
                     /locus_tag="VFD06_09385"
     CDS             complement(4761..5552)
                     /gene="murI"
                     /locus_tag="VFD06_09385"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZN03782.1"
                     /translation="MIGVFDSGIGGLTVYQALRRSLPAEERLLYLGDTARVPYGTKGD
                     ATVARYAVEGARFLARRGVKALVVACNTMSAVALPSLTGIVPGPVLGVIEPGVEAACR
                     ATRGGAIGVIGTAATIKSGAYPAAIVRRLPGARILDRACPLFVPLAEEGWTDNAVARA
                     AAAEYLADLPERGLDTLVLGCTHYPLLRGVIAATVGPSVALVDSAEETAKTVRALFPS
                     DGGTPATGDETDHFYVTDSSERFREVGSRFLGRPLQRLELVDLAQ"
     gene            complement(5549..6232)
                     /locus_tag="VFD06_09390"
     CDS             complement(5549..6232)
                     /locus_tag="VFD06_09390"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZN03783.1"
                     /translation="MKRAAALALVLALAGCGGGREDSRDGAENAEEAASVTGAPQAAS
                     PAGAPAPEGAAPAAGEGTAGSAAPAPEDQAALAGEEEHRREVLLFFQKKDDDLLAPET
                     RRILPGTSVVDQAKQLVAELIAGPRTNGLLPTVPERTTVLGLYLDRSGTAFLDLSDEF
                     VTGHPGGSSEELATIFSIVDSLTYNLPEIKRVRFLVGGEERETLHSHLDLRRAYGKNM
                     SMVKMEGGG"
     gene            complement(6229..7509)
                     /locus_tag="VFD06_09395"
     CDS             complement(6229..7509)
                     /locus_tag="VFD06_09395"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZN03784.1"
                     /translation="MPGRRRRHAALAAAPPLLPRLLAAFLLLAMPAAAVAADPVAFTL
                     RTVDSEVGTRVIIEFSRRPVYEVRGERDRIYITLREGEVRPPFKKRDFDGPLLERIKF
                     AEGMRTSEIVFYAGPQFGTFSTFEMGEPFRLVLDLRRRSAAPPATRIDPGSQAGQGVS
                     PGLPPPPGGAPAPQAPPGTAPPHGAAPSGQTSPPVETRPRRAAFTVVIDAGHGGDETG
                     ARGPSGLLEKDVTLDVARRLKARLAGEDGIAVFLTRDDDRKVPLDDRTALANHERADL
                     FVSIHANSSRRDSARGAETFFLSYQATDDDARALAALENDTLGIERNVGGQSGLDLVL
                     WDLAQSAFLKESSDLAEEIQDRLNDTLGVRNRGIKQAPFKVLMGATMPAVLVEIAFIS
                     SPEEERRLRESAFKDRIAEAIASSIRKFRERTAR"
     gene            complement(7521..9506)
                     /gene="asnB"
                     /locus_tag="VFD06_09400"
     CDS             complement(7521..9506)
                     /gene="asnB"
                     /locus_tag="VFD06_09400"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN03785.1"
                     /translation="MCGIVGVADFRGGAGPDPGLLAAMTDTLAHRGPDGRMVLRLDNG
                     GMPQLTFGFRRLSIIDLGAGARAYSDESGRIRVICNGEIYNWPELQADLEKRGHRFET
                     RCDTEVLPHLYEEYGLDFVSRLNGMFAFAIFDARAERLVLGRDRVGEKPLYYVDRPGE
                     LLFGSEIKALLAHPDVSRERDLEGLTRYLLYGYFPAPWTPFRDIRKLPAGSLLIAERG
                     RVRVEPYWSPARFVPAAPGARGTGAAPAAIPSVEEAATEVRRLLSEGVALRLRADVPV
                     GVFFSGGVDSTAIAALAVEIHGKGVPTFSLGFEDADFDESGYARKAAALLGTEHHETM
                     AGEKDLVGALYGVARVLDEPLADASVLPTWLLSRFARRHVKVTLGGEGGDELFAGYPT
                     YIGDRLARLYNRLPRAVRLLVRRAVEAAPAGFSNVGLEFLLKKFVASADRPDDERHPI
                     WFGAFPPERQRELLSDGALAVLDQRPGHDPLREARAILGRAAPFQHPLDALLLTDFTL
                     YLQDDLLTKIDRASMAASLEVRAPFLHHPLVEYVFGLPASYKLRGHRTKWILKRALAG
                     KLGAETLRRRKRGFNIPLARLLLGPLDPLLKRAFDPARLRREGLLRPETVGALWEAHR
                     TRRRDHGRLLWTLLMLQLWLSRHESNGDLLDAPGGAC"
     gene            complement(9512..11320)
                     /locus_tag="VFD06_09405"
     CDS             complement(9512..11320)
                     /locus_tag="VFD06_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN03786.1"
                     /translation="MKRIQWLAPLLCAPLFLAGLGGTGLADPDEGRNAEVGREMLASG
                     DYVTPRLNGAVYLDKPPVFFWMVAGSLAAFGTNEAAARLPSALAAVAGVALTVWFARR
                     RFGAPVAACAAAILALSPLYQVFARLVIFDMLLLVWTTTCTFLFYEAMEGERPSRWLG
                     VAGFVVAGLGTITKGPVALVVPLLVAVAWALVERRPSLLRRLRPGAGMLAYAVVVLPW
                     LLVVERRHPGFLAYAVLGENLARMTANPYDTARPFHFYLQVILPGLFPWILLLAAQGI
                     GLAWSRLPGRQTAPAAAPHAGSPGAAAQDAAPPGEARAARFVALWLAVIVVFFSLVAS
                     KRPSYILPASIPVAILAARLIVRGSRAATRDAALRAAGTVTAAVATIVLAATAWVAAS
                     GGGPVALGRVGRAARDSGVPPILLWAAAAALLVGAALLLAVRRSARPAIYVAVAALPL
                     VALLPVARFAVRHIDSLRSSRAVSRFLQERLADRDRVVCFEEFRPGLNFYLERPIGQV
                     TRAGRIFTSNYIAAHLDAQRADPGFRLMEPAALQAALRDPAALTYVLAPRKEFDALRR
                     TAGLPLQAIWEGKGFGLFLPAAEVTGDNHPLPRAGG"
     gene            complement(11317..12426)
                     /locus_tag="VFD06_09410"
     CDS             complement(11317..12426)
                     /locus_tag="VFD06_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HZN03787.1"
                     /translation="MARKGRGAVSNLQGRFERDLREAFDDGWGSAPEEAPRTIEAAPS
                     PERTPLPPLRTEVHIERARSVLTRNASPDIPFDVSLNPYRGCEHGCIYCFARPTHAYL
                     GLSPGLDFETKLFSKPDAAALLRAELAKPGYVCKVTALGANTDAYQPIERRLEITRSV
                     LEALREHRHPVLVITKSDLVLRDLDLLAPMARDRLAAVHVSVTTLDRTLARRMEPRAA
                     TPARRLEAIRRLSEAGVPVGVLAAPIIPGLNDSELEAILAESKAAGAVSAGYVLLRLP
                     LEIKDLFREWLEQHYALKADRVLALLRDMRGGRLYEATYGKRMRGEGPHADLLRQRFE
                     VAIRRHGLGHPDIALDETLFRVPPRPGQQGTLFPT"
BASE COUNT         1885 a         4495 c         4505 g         1627 t
ORIGIN      
        1 ccccgcccgg cgggacgagc gtgtagcgga agaccgcccg gcggtccact acgagcggct
       61 tgaagcgcag gtcccaggcc gagacggccg cggatgcttc gacctgccag accggcgcct
      121 tgaagaagcg ttcgagcctg cggccgtcgg tggtttgacg cgcggcggcg acgcgggggt
      181 cgtcgaggtc gttccggacg gtcccccgcg gctcgacttg ccccaccagc ggccggatca
      241 agaagaggtc gtagcggccc ggggagcgga ccacaccacg ccagcggtgc ggcccgagcg
      301 gctccgggaa gacataggcg aagtcggccc cggaaggggc gctggcctcg aggagcgtct
      361 cggcgcgcca ccgggcggcg ccgcaggcca cgaggtagat cgccacggcc gcgagcgccc
      421 cccgcgagag tgaccgcagc cgcggtctca ggcggggcag cagcgccagg gccagaggca
      481 gagccagcag gccggtcagc acgaaatcga tgatgaagac ccacgcgagc tccggccgga
      541 cgtcggacaa cggccagaag agcacgaccc cgaacgagtt gatgagatcg aagagcaggt
      601 gcccggcgac cccggcgagg gtcatcccgt agagcgtccg cagcggcacc tcgcgatacc
      661 ggcggccgag gatccacgcc aacgccagcg cccagaacgg caggaggaac agagagtggc
      721 cgaaggtgcg gcgcatcagg acggcgtagg ggtcagaggt caagtggacg atgccgtcga
      781 gatccgggag gttcgaggcg agcaggaggg tgggcagagc ggcccggccg acccgctccc
      841 ggaagaggcc gttcgccagg ccggcgccga gcagcgtgtg ggtgatcgga tccatcgcgg
      901 tcctggcggg gatcggctcg ggctccgccg cgggttacag accgaactcg ctcttccgga
      961 agaggggctg gaagcgccag cgggcgaggg cgtcggcccc cccctgctcc cggtccacga
     1021 ccgcgaccac ggcggcgatg tcgaggccgg ccgcctcgat cttgtcgatc gccgccttgg
     1081 tggacttggc cgtggtgacc acgtcctcga agaccaggac gcgcgagccc atcgggatgt
     1141 cgttctcgat cagccgctcg gtgccgtggt ccttgctcgc gctgcgcacc aggaaggccc
     1201 gcaccggatg gcctttcccg gcctggtgcc gctcccagga gcggccggcg acgtggccga
     1261 cgaccgggtc ggcgccgatg gtcgggccgc cgatggcgtc gatgggccga ccatcctgct
     1321 gctccagccg atcgatctgc tccaacagca gcgtccccag caggtgcagc ccctccgggt
     1381 cgagggtggc gcgcttgcag tcgaagtaaa cgctggactt ctgcccggag gcgagggtga
     1441 actccccggt caatatgcat ttctgttcga tgagggcctt cagccgcgcc ctggggtcca
     1501 ctcggtctcc ctccgccgga tccggccgcc gggggccgga ttcccaccgt ataataacga
     1561 tgcgttcacg gcgatgtcat ccgagcccga cctgaccgat tcccgctcca ccctgatgca
     1621 ggatcctccc cggcgtcgcc cggtgtccct gagcgcctat ctcgtggcgc gcctgctgcg
     1681 gctccgcccg aaccacgggc cgcggccgat gggccaggtc aagcgtgacc agctcgacgc
     1741 gccgcgctgg gccctgacct tggggatcct gctcttcctg atcctcttcc tgaccctcat
     1801 catcctgttc ttcgtcgccg ctccgcccgt ctccgccgcc tccgacgggg tgaacttcga
     1861 caagccgacg aaggactggt accaggggcc ggtccgctac atcatcacca aggtcgagac
     1921 gaaggcgtac aaggcgctcg agaccgaatc ggagcggctc actttcatcg actggttctg
     1981 gcagcgccgc gacatcgagc cgtccacccc gcagaacgag ttccgcgacc gctacgagca
     2041 gcgggtcttc gaggcgcagc gcaagttcat cagcagcgcc accccgggat ggaagaccga
     2101 catggggaag atctacatcc tcgtcggccc gccggacgag atcaacagcg acgtcgtggc
     2161 gcagacgcat cgcggcatca tcacctgggt gtaccggcgc ccgccgttcc ccgacatgca
     2221 gccgaacacc gtcatcgggt tcgcgcgcga ccccagcggc gagttccggc tgagtatcaa
     2281 cccgaccatc gactccgacg tggcgcgcgg cctgaagttc tcgcgcacca tgatcacggc
     2341 cgaccagacc ctcatccgcg ccgggcaggt ggaccccgcc ctcctggcgg cgggggcgac
     2401 cctgtcggcg agccagatcg agaccaacat gacctacggg cgcatgcaga tgctgccgcc
     2461 tcacgaggag gagctgttca agagcttcgt ctcgtcgcgc gagtcgtacg gcagcaccat
     2521 cccgatggag acccgcttcg acttctaccg cgccggcgac gacacctaca ccacggtcac
     2581 ggtcggcatc cgcagcacgg cagtgcagta ccgcacccgc ggcggcaagg aggtgccgga
     2641 cgtgggggtc ttcggcaagc tcgtcagcaa ggagaacccg gagctctcct acccgttgtc
     2701 gagcgagagc ggcttcgccg acagcccgga gaacgcctcg gccgggatcg gcgacctgct
     2761 catcttccag gcggtcggcg cgttcaagcc gggactctac cgcgccatcc tcggcgtgga
     2821 ggatcgcgtc agcaacaagc tctcgtcgct cgtgaaggag atcgaagtgc ccgacctcac
     2881 gggcaccgcg ctgcggctct cctcgatcac cgtcgccggc tcgatggagc cgaccgagta
     2941 cgccacgtcc accgccaagc cgttccagat cggcaagttc aagatcgtcg cgcgccccga
     3001 cgccgcgttc tcgaagagcg acgagctgga cgtgtacttc caggtgtacg ggccggccag
     3061 cgacccggcg accgggaagc cgaagatgga cgtcctgtac agcttccggc gccgcgccga
     3121 ggacggcacc taccaggacg tcggaaccta cagggtggcc gacagcccgg cgcaggtgca
     3181 ggggtatgcg gtgccgctgg agaaatggcc cgaggggacg tacaccctga ccgtcaccat
     3241 cgtggacaag gtggcgaagg cgcaggcgac cggcgacgcg atcttcacca tccgtccctg
     3301 atcggggccg tcaccgcgcg gccaccccgg cgccgcgcag aaactgctcc ccttccccgc
     3361 tcccgaggaa ctccgtcagg aggcgaaacg ctgcgccccg gtgcgacacc gcgtgcttgc
     3421 gcgcgtcgcc gacctcggcg agggtcgcgc cgtacggcgg atagaagaac aaagggtcgt
     3481 agccgaagcc cttctccccg cgcggcgtcg tggcgatccg gccggggcac tcgccgcgga
     3541 acgagcgcgc ctccccgtcc ggacgggcga ccacggccac cgccacgtac cgggcccccc
     3601 tccgatcgtc cggcacgtcc gcgagggccg cgagaagcgc ccggttgcgt ccggcatcgt
     3661 cgagccgcgg tcccccgtag cgggcggaca gcggtccggg cgcgccgccc agcgcctcca
     3721 cctccagccc ggagtcgtcg gcgatcgtca ccttccccga caggcgggcg taatggcggg
     3781 ctttgccgag gccgttctcc tcgtaggtgt tcccggtctc ctcgtacggc tcgcggatgc
     3841 cgagatcggc gagcgtgacg gcgctgcggc cgaaggcctc gagcagcgcc tgaagctcgc
     3901 gcgccttgcc gggattgaac gaggcgatga gaagtgccgg ctccgcgtcg ccctcggcgc
     3961 ctccggacgg ggacgcgcgg gtcacgtccg gggcaggagg ggcggcagga cgtcgtgcag
     4021 gacctcccgc tgcacctcga acatgcggtc ggtcccgagg accgcgaggt cgagcagccg
     4081 ctgcatctcc tcgagcgaga agggatgctt ctcggccgtc ccctgcacct cgacgaaccg
     4141 gccggcgtcg gtgcgcacca cgttcatgtc cacgtccgcc tgggaatcct ccacgtagga
     4201 gaggtcgagg caggcgagcc cgccgacgat gccgaccgag gtggcggaga cgagcccgcg
     4261 caggaccgga ccgcccagca ggttcttcgc ccgcagcttc gcgagcgcga gggcgagcgc
     4321 gacgtacgca ccggagatcg ccgcgcagcg ggtgccgccg tccgcctgca ggacgtcgca
     4381 gtcgatccaa acggtgcgct gtccgagcgc tttcgcatcg accacggcgc gcagcgaccg
     4441 gccgatgagc cgctggatct cctgggtgcg gccggaggga cgtcccttgg tcgattcgcg
     4501 cgacgtccgc tcgttcgtgg cgcgcggcag catgccgtac tcggcggtga cccagccctg
     4561 ccccgtgtcg cgcatgaacg ccgggacccg gtcctcgacg ctcgccatgc acaggacgtg
     4621 cgtctggccc accttgatga gcgccgatcc ctcggcatgc atgttcacgc cggtcaggat
     4681 ctcgatcggc cgcccctgtc gttcgtcccg tccgtccagt ctctgcatcg tcgtcgctca
     4741 cggccgccgg gccgcctccg tcactgcgcc aggtccacca gctccagccg ctgaagcggc
     4801 cgccccagga agcgcgatcc gacctcgcgg aagcgctccg acgagtcggt gacgtagaaa
     4861 tgatccgtct cgtccccggt cgccggcgtc ccgccgtcgc tcgggaacaa ggcccggacc
     4921 gttttcgccg tctcctccgc cgaatcgacc agcgcgacgc tcggcccgac cgtggcggcg
     4981 atgacgccgc gcagcagcgg gtagtgggtg catccgagca ccagcgtgtc gaggccgcgt
     5041 tccggcaggt ccgcaagata ctccgccgcg gcggcgcggg ccacggcgtt gtcggtccag
     5101 ccctcctcgg ccagcggcac gaagagcggg caggcgcgat cgaggatccg cgcacccggc
     5161 aggcgtctca cgatggcggc gggataggcg ccgctcttga tggtggcggc ggtgccgatg
     5221 acgccgatgg cgccgccgcg cgtggcgcgg catgcggcct ccacccccgg ctcgatcacg
     5281 cccagcaccg gccccgggac gatcccggtc agcgacggca gcgccaccgc cgacatggtg
     5341 ttgcaggcga ccacgagcgc cttcacgccc cgccgcgcca ggaagcgcgc cccttcgacg
     5401 gcgtagcgcg cgaccgtcgc gtcccccttg gtgccgtacg ggacgcgcgc cgtgtcgccg
     5461 agatagagga gccgctcttc cgccggcagg ctgcggcgca gcgcctggta gacggtcagg
     5521 ccgccgatgc cggagtcgaa caccccgatc atccgccccc ctccatcttc accatcgaca
     5581 tgttcttccc gtaggcgcgg cgcagatcga ggtggctgtg aagcgtctcc cgctcctccc
     5641 cgccgacgag gaaccgcacc cgcttgatct cgggcaggtt gtaggtgagg gagtccacga
     5701 tggagaagat ggtcgccagc tcctcgctgc tcccgccggg gtggccggtg acgaactcgt
     5761 cgctcaggtc gaggaaggcg gtgccggagc gatcgagata cagccccagc accgtggtgc
     5821 gctccgggac ggtcgggagg agcccgttcg tgcgcggccc ggcgatcagc tccgcgacca
     5881 gctgcttcgc ctgatcgacg accgacgttc cggggaggat gcgccgcgtc tccggcgcca
     5941 ggagatcgtc gtccttcttc tgaaagaaga gcaggacctc gcgccggtgc tcctcctccc
     6001 ccgccagggc cgcctggtcc tccggggctg gcgcggcgga gccggccgtt ccctctccgg
     6061 ccgccggggc ggccccttcg ggagccggcg cgccggccgg cgacgccgcc tggggggctc
     6121 cggtcacgga agcggcttcc tcggcgttct ccgcgccgtc ccgcgagtcc tcccggccgc
     6181 cgccgcagcc ggcgagcgcc aggacgagtg cgagcgccgc cgcccgcttc accgcgcggt
     6241 ccgctcgcgg aacttgcgga tgctcgaggc gatcgcttcg gcgatgcggt ctttgaacgc
     6301 cgactcccgc aggcggcgct cctcctccgg gctgctgatg aaggcgatct ccacgagcac
     6361 cgccggcatc gtcgccccca tcaggacctt gaacggagcc tgcttgatgc cccggttgcg
     6421 gacgccgagg gtgtcgttga gacggtcctg gatctcctcc gccaggtcgc tcgactcctt
     6481 gaggaacgcc gactgcgcca ggtcccacag gacgaggtcg aggccgctct ggcccccgac
     6541 gttgcgctcg atcccgagtg tgtcgttctc cagcgccgcc agcgcccgcg cgtcgtcgtc
     6601 ggtcgcctgg tacgagagga agaaggtctc cgcgccgcgg gcggagtcgc gccgcgatga
     6661 gttggcgtgg atgctgacga agagatccgc ccgctcgtgg ttggcgaggg cggtccggtc
     6721 gtcgagcggc accttgcgat cgtcgtcgcg ggtcaggaag accgcgatgc cgtcctcgcc
     6781 ggcgaggcgc gccttgaggc gccgcgcgac gtcgagggtg acgtccttct cgaggagccc
     6841 gctcggcccg cgcgcgccgg tctcgtcgcc gccgtgtccg gcgtcgatga cgacggtgaa
     6901 cgccgcccgc cgcgggcgcg tctcgaccgg cggggaggtc tgcccggacg gcgcggcgcc
     6961 gtgagggggg gcggtcccgg gcggagcctg gggcgccggc gcgccgccgg gcgggggcgg
     7021 aaggccgggg gagaccccct gtcccgcctg cgatccggga tcgatgcggg tggccggcgg
     7081 agcggccgac cgccggcgca ggtcgaggac gagccggaac ggctccccca tctcgaaggt
     7141 cgagaaggtg ccgaactgcg ggccggcgta gaagacgatc tccgaggtcc gcatcccctc
     7201 ggcgaacttg atccgctcga gcagcgggcc gtcgaagtcg cgcttcttga acggcggccg
     7261 cacctccccc tcgcgcagcg tgatgtagat ccggtccctc tcgccgcgca cctcgtacac
     7321 cggccggcgg ctgaactcga tgatgacgcg ggttccgacc tcgctgtcca ccgtgcgcag
     7381 ggtgaaggcg accggatcgg cggcgaccgc ggccgcgggc atcgccagca ggaggaacgc
     7441 cgcgaggagc cgcggcagga gcggcggggc ggccgcaagg gctgcgtgac gacggcggcg
     7501 gcccggcatg gggccgcaat ctagcatgcg ccgccgggag cgtccaagag gtcgccgttc
     7561 gattcgtggc gcgacagcca gagctgcagc atcagcaggg tccagagcag gcggccgtga
     7621 tcgcggcggc gcgtccggtg cgcttcccac agcgcaccca ccgtctccgg ccgcagcagc
     7681 ccttcgcgcc gcaggcgcgc cgggtcgaag gcccgcttga gcagcggatc gagcgggccg
     7741 agaagaagcc gcgccagggg gatgttgaag ccacgcttgc gccgcctcag cgtctcggcg
     7801 cccagcttcc cggcgagggc ccgcttgagg atccacttgg tccggtggcc gcgcagcttg
     7861 taagaggccg gaaggccgaa gacatactcc accagcggat ggtgcaggaa cggggctcgc
     7921 acctcgagcg atgccgccat cgaagcccga tcgatcttgg tgagaaggtc gtcctgcagg
     7981 tacagcgtga agtcggtcag gaggagcgca tcgagcggat gctggaatgg ggcggcgcgg
     8041 ccgaggatcg cccgcgcctc gcggagcggg tcgtgccctg gcctctggtc gagaaccgcc
     8101 agggcgccgt cggacaggag ctcccgctgg cgctcgggcg ggaaggcccc gaaccagatc
     8161 gggtggcgct cgtcgtccgg ccgatcggcg gaggcgacga acttcttgag gaggaactcg
     8221 aggccgacgt tcgagaagcc ggccggggcc gcctccaccg cccgcctcac caggaggcgc
     8281 acggcgcgcg gcagccggtt gtagaggcgc gccagccgat cgccgatgta ggtcggatag
     8341 ccggcgaaca gctcgtcccc tccttccccg ccgagcgtca ccttgacgtg acggcgcgcg
     8401 aagcgggaga gcagccaggt cggcagcacc gacgcgtcgg cgagcggctc gtcgaggacg
     8461 cgcgccacgc cgtacagcgc gccgacgagg tccttctcgc cggccatcgt ctcgtgatgc
     8521 tcggtgccca gcagggccgc cgccttccgc gcgtaaccgg actcgtcgaa atcggcgtcc
     8581 tcgaagccga gggagaaggt cggcaccccc ttgccgtgga tctccaccgc gagcgcggcg
     8641 atcgccgtcg aatcgacccc gccgctgaag aagaccccga ccgggacgtc ggcgcgcagc
     8701 cgcagcgcca ccccttccga caggagccgc cggacctcgg tggcggcttc ctccacggag
     8761 gggatcgcgg ccggcgccgc gcccgtcccg cgcgcgcccg gggccgcggg aacgaagcgg
     8821 gcgggcgacc agtacggctc cacccggacg cggccgcgct cggcgatcaa gagagaaccg
     8881 gccggcagct tgcggatgtc tcggaagggg gtccaggggg ccgggaagta cccataaaga
     8941 agatagcggg tgaggccctc gagatcgcgc tcccgcgaca cgtcgggatg ggcgagcagc
     9001 gccttgatct ccgatccgaa cagcagctcc ccgggccggt ccacgtagta gagcggcttc
     9061 tcccccaccc ggtcgcgccc cagcaccagg cgctcggcgc gggcgtcgaa gatggcgaac
     9121 gcgaacatcc cgttgagccg cgagacgaag tcgagcccgt actcctcgta gaggtgcggc
     9181 aggacctcgg tgtcgcagcg ggtctcgaag cgatggccgc gcttctcgag gtccgcctgc
     9241 agctccggcc agttgtagat ctcgccgttg cagatgaccc ggatgcggcc cgactcgtcc
     9301 gagtaggcgc gcgccccggc gcccagatcg atgatgctga ggcggcggaa cccgaacgtc
     9361 agctgcggca tcccgccgtt gtcgaggcgc agcaccatgc ggccgtcggg gccgcggtgc
     9421 gcgagcgtgt cggtcatggc ggcgagcagc cccggatcgg gcccggcgcc gccccggaaa
     9481 tcggccacgc cgacgatgcc gcacattgcc gctatcctcc cgcccggggc agagggtggt
     9541 tgtcccccgt gacctcggcg gccggcagga acagcccgaa ccctttcccc tcccagatcg
     9601 cctgcaacgg caggccggcg gtccggcgga gcgcgtcgaa ctccttgcgc ggtgccagca
     9661 cgtaggtgag ggcggccgga tcgcgcagcg ccgcctgcag cgccgccggt tccatgagcc
     9721 ggaaccccgg atcggcgcgc tgggcgtcga gatgcgcggc gatgtagttg ctcgtgaaga
     9781 tccgcccggc gcgcgtcact tgaccgatcg gccgctccag gtagaagttc agcccggggc
     9841 ggaactcctc gaagcagacg acccgatccc gatcggcgag ccgctcctgc aggaagcggc
     9901 tgacggcgcg cgaggagcgg agcgagtcga tgtgacgcac cgcgaagcgc gccaccggca
     9961 gcagggcgac gagcggcagc gccgcgaccg cgacgtagat cgccggccgg gcggagcggc
    10021 gcacggcgag gaggagcgcc gcgccgacga gcagggccgc cgcggcggcc cacagcagga
    10081 tcggcgggac gccggaatcg cgtgctgccc gtcccacgcg cccgagcgcg acgggcccgc
    10141 cgccgcttgc cgccacccag gcggtggcgg ccaggacgat ggtcgcgacc gccgccgtca
    10201 ccgtgccggc cgcacgcagc gccgcgtcgc gcgtcgccgc tcgcgagccc cggacgatca
    10261 gccgggccgc gaggatggcg accgggatcg acgccggcag gatgtacgac ggccgcttcg
    10321 aggcgacgag ggagaagaag acgacgatca cggcgagcca cagggcgacg aagcgcgccg
    10381 cgcgggcctc gccggggggc gccgcgtcct gcgcggccgc tccgggcgac cccgcatgcg
    10441 gcgcggcggc cggagccgtc tgacggccgg gcaggcggct ccaggcgagg ccgatcccct
    10501 gcgccgcgag gagcaggatc cacgggaaca atcccggcag gatgacttga aggtagaagt
    10561 ggaacggacg cgccgtgtcg tacgggttgg cggtcatccg cgccaggttc tcccccagca
    10621 cggcgtacgc caggaatccc gggtgccggc gctccacgac gagaagccaa ggcagcacga
    10681 ccaccgcgta cgcgagcatg ccggccccag gccgcagccg ccgcaggaga gacggccgcc
    10741 gctccacgag cgcccaggcg accgcgacca gcagcggcac cacgagcgcc accggcccct
    10801 tggtgatcgt cccgagtccg gcgacgacga accccgccac ccccagccac cgggaaggac
    10861 gctccccttc catcgcctcg tagaagagga aggtgcacgt cgtggtccag acgagcagga
    10921 gcatgtcgaa gatcaccagt cgcgcgaaca cctggtagag cggggacagc gccaggatcg
    10981 ccgcggcgca ggcggcgacg ggcgcaccga agcgccgccg cgcgaaccag acggtcagcg
    11041 ccaccccggc gacggcggcg agggcggacg gcagccgcgc ggccgcttcg ttcgtcccga
    11101 acgcggcgag cgagcccgcg accatccaga agaagaccgg cggcttgtcg agatagacgg
    11161 cgccgttgag gcgcggcgtc acgtagtcgc cgctcgcgag catctcgcgg ccgacctcgg
    11221 cgttgcgccc ttcgtcggga tcggccagtc cggtgccgcc gagtccggcg aggaacagcg
    11281 gggcgcagag aagcggcgcc agccactgga tccgcttcat gtcgggaaga gcgtcccctg
    11341 ctgtccgggc cgcggcggca cccggaacag agtctcgtcg agcgcgatgt ccgggtgccc
    11401 gaggccgtgc ctccggatcg cgacctcgaa gcgctggcgc aggagatcgg cgtgcggccc
    11461 ctcgccgcgc atccgcttcc cgtacgtcgc ctcgtagagc ctcccgccgc gcatgtcgcg
    11521 cagcagcgcc aggacgcgat ccgccttcag cgcgtagtgc tgctcgagcc attcgcggaa
    11581 caggtctttg atctccagcg gcaggcgcag caggacgtag ccggccgaga ccgcccccgc
    11641 ggccttgctc tcggcgagga tcgcctcgag ctcggagtcg ttcaggccgg ggatgatcgg
    11701 ggcggccagc acccccaccg ggacgccggc ctcgctcagg cggcggatcg cctcgagccg
    11761 gcgcgccgga gtcgcggcgc ggggctccat gcgccgcgcc agcgtccggt cgagcgtcgt
    11821 gaccgagacg tggacggcgg cgagccggtc gcgcgccatc ggagcgagca ggtcgaggtc
    11881 gcgcagcacc aggtccgact tggtgatgac gaggaccggg tgccggtgct cgcgcagcgc
    11941 ctcgagcacc gagcgggtga tctccagcct ccgctcgatc ggctggtagg cgtcggtgtt
    12001 ggcgccgagc gccgtcacct tgcagacgta gcccggcttc gcgagctccg cgcgcaaaag
    12061 cgccgccgcg tccggcttgg agaagagctt cgtctcgaag tcgagtccgg gcgacaggcc
    12121 gaggtaggcg tgcgtcggcc gggcgaagca gtagatgcag ccgtgctcgc aaccgcggta
    12181 ggggttgagc gagacatcga acgggatgtc gggggatgca ttgcgcgtca ggacagaacg
    12241 ggcgcgctcg atatggactt ccgtgcgcag cggcggcagc ggggttcgct ccggcgacgg
    12301 tgcggcctcg atggtacgcg gcgcctcttc cggtgcactg ccccagccat cgtcgaacgc
    12361 ttcgcgcagg tcgcgctcga aacgcccctg caggttgctc acggcgcccc ggcccttgcg
    12421 cgccacgggc ggccgcgggc ctgcctgcgg cgcgagtgca tccgggtcgg catcgggccg
    12481 cgtcggtcgg ctgctggcgg acttgggttt ct
//
LOCUS       DASRIQ010000387        14623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3022732, whole genome shotgun sequence.
ACCESSION   DASRIQ010000387 DASRIQ010000000
VERSION     DASRIQ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 14623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14623
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022732"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3772)
                     /locus_tag="VFD06_09415"
     CDS             complement(<1..3772)
                     /locus_tag="VFD06_09415"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN03788.1"
                     /translation="MLKRYALGGTLALFVCLGHVRAQTSGGIRGRVVDLSGQPLISVL
                     LSVSSRTLQIDRPIGRSDAEGRFQAIALDPSDDYVVKAALPGYATVATPDIAVRAGQM
                     TTVTIALLPESSVREQVKVTGTASPVSLEERGLTTHISSEFIDNLPILGRNYQEVLTL
                     SPGVSDVDGDGNPNIHGARDTDVGTFVDGVNTTDPLTGKVGAQLNIESIQEIEVKTAG
                     ASAEFGRAQGGFANILTKSGGNDFEGAFKFYWRGSALDGDGAGTDDSSLHGGVGEVGL
                     RDLTFNDYLPFLSLSGPIVKDKAWYFVTLEYIQREDPVNAVTQAFVTGLEEKRLFAKG
                     TWQATANTRLVFSVNYDPQEFTNQGLNSLTRQEAGYALKAGGPLYSARGTSVLGPSVA
                     LETTVSYFDGRPAVVPNLPLDGNGDGILYIDRNGDGFRDARERDPGEDLDGDGRFDVW
                     EDTLVPNGQLDQLEELFCFIPPTLERPGFWGHSPPFTCSAPLLFLSDEDGARIDPLFP
                     QVLPGDGDRRLTPPGGCEGISREDRDCDAHLDARNEDRNRNGILDLGEDLDGDRRLDD
                     GTEDRNRNGRIDDTPYPTGPYPYGRLAPVPGDRDYSIDMRKGVIEGPYYQEYEDQRER
                     ATLRQDLSVFVGGFRGAHDIKGGYLLEKEQFARAADGHPVNAVNDPGWRTGNLLDRVR
                     NPGVHFDCDPYLDPCIDPGFGRVTALLPTTPSVDQSAESLSGGVYVQDTWKPIPNVSL
                     SLGLRFDREAATTSGYTSFEPEAERERLDRLLALAGAEAGQDDLTVGNNDGIESRGIP
                     SDPLFGGAATGTVTEPIVNGLKRAAYASLTLHRSSAEFSLAQLNAFFPGLVKDGQFDQ
                     AVAASYGIQIQQPETFRITNNNLSPRLGASWDPQGDGRTKVFGAWGRYYDRLFLNTIV
                     GEQGLETIQRYYVLDRDAFVPTIIPQTGAVTGGVPNHGYGTIVSISPPTVTQVDRDLK
                     TPYCDEWILGFEREIAAETALSVRAVQRRYKDQLQDVDVNHETRVDPFTGELSDRFGI
                     ATEVVSTDDPDAPSVITRGSDGRPDLFVRNYFFNQVLRVGNTNSARYNAFEVEVRRRM
                     ARRWQMQGSYTYSRAIGAAEEFQSRLGNDPSIVESEFGYLDFDQRHVVKLNAGIFLPG
                     DWQVGVASQWASGLPYSIVSRFFALDNVDYQQFRTTYGFTELDGERLKFRSIARNSER
                     NGSTLNIDGSVRKNFVWGRTTWGVSLEVFNILNSDELRIYSFEPSRSTGFDAG"
     gene            complement(3835..4374)
                     /gene="folK"
                     /locus_tag="VFD06_09420"
     CDS             complement(3835..4374)
                     /gene="folK"
                     /locus_tag="VFD06_09420"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZN03789.1"
                     /translation="MTGKRRGDRSGEAGRTACIGLGSNVGNRRRLLERAVDRMQEAGL
                     RVVARSSIYRTEPVDLLDQAEFLNQVVAVRTPLEAPDILAICLDIERGMGRVRRQDKG
                     PREIDLDLLLLGEVVLEQEGVEVPHPRLHLRRFVLVPLAEIAPDAVHPVLGLTIAELL
                     ARCPDRSSVVRLESGTKTG"
     gene            4561..5118
                     /locus_tag="VFD06_09425"
     CDS             4561..5118
                     /locus_tag="VFD06_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03790.1"
                     /translation="MKKPTKKVAKSPAPAVAGGNTWNIQEKALKEMERGLQAMHKQSW
                     ADARTHFQTIIDGYPNEKELQDRAQMYARVCRNHIESDGGSQRAKPEDLFYLGVMKSN
                     EADFDQAVELLDRALQHNPKDERSHYVMAATRALKGDRDVALKHLQEAISLNVQNRFY
                     AMNDPDFESIRDEETFENLVYPEEA"
     gene            5396..6787
                     /gene="glmU"
                     /locus_tag="VFD06_09430"
     CDS             5396..6787
                     /gene="glmU"
                     /locus_tag="VFD06_09430"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZN03791.1"
                     /translation="MALILAAGLGKRMQSRWSKLLHPVAGRPVVRHVVEATRGAGVAR
                     TVVVIGNQAEEVRRAVGDHDKRIAFAFQKEQLGTGHAVLSAERMMHGHDGDVLILNGD
                     LPALRPETLVAFVDYHRAHAAPLSLLTTVLADPRGYGRVIRNYGGDVSRIVEDSDASA
                     DERATQEINCGIYCADSRSLFRPLRQATRNNAQGEIYLTDLIEILRKEGRKVAAYRHP
                     EAGEVLGVNDRRELAEAGRSLYRRKAETLMRSGVTLIDPDTTYIDMQATVGRDSIIEP
                     GVMILGDSHLGDDVRVGLGSRLVDTTIGDGTEILAYCVIGSSKVGRGCRIGPFSHLRP
                     ESALDDEAHVGNFVETKKTRLGRGSKANHLSYLGDAEIGRDANIGAGTITCNYDGVAK
                     HRTIIEDEVFIGSDTQLVAPVRVRRGAFVGAGSTITKDVPAHALAISRVRQENKEGWA
                     KKHGPLAKRDGHK"
     gene            7046..8887
                     /gene="glmS"
                     /locus_tag="VFD06_09435"
     CDS             7046..8887
                     /gene="glmS"
                     /locus_tag="VFD06_09435"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZN03792.1"
                     /translation="MCGIVGYIGAKKVSSVILDGLRRLEYRGYDSAGIAVIDGPNVKV
                     VRAAGKLRELEENLRLTQLDGSFGLGHTRWATHGRPTEENAHPHRDCSGSMVVVHNGI
                     IENYKELKTQLIGLGHQFKTETDTEVIAHLIEEVRKGKSDMPLEDGVRGAVPRLTGSY
                     AFAAVSTKDPRKIVAARNGPPLVIGLGKDEYFVASDIPAILHHTRDVFFLDDREVAVV
                     TDGGVVITDHDGKSIRKQVQRITWDPILAEKAGFKHFMLKEIYEQPRAIRDTLLGRVS
                     QDTGKIFLDEMEVTEQEFRDCRKLHIVACGTSWHAALIGKFLIEKLAKLPVEVDIASE
                     FRYRSPILEPDHLLLVITQSGETADTLAAQREARSRGLKTLAICNVVGSMATREAQGV
                     IYTHAGPEIGVASTKAFTAQIAALYLLALHTAQVRGALTPEECKSHIRDLLLIPTQIE
                     QILAKDGEIEEIAKDFHRASDFLYLGRGVNYPIALEGALKLKEISYIHAEGYPAGEMK
                     HGPIALIDRNLPVVALVFGDALFEKMMSNVEEAQARDGAIIMVTQDGHEEAGRRCART
                     VTIPKTSDLLTPILASIPLQLLAYHIALRRGCDVDQPRNLAKSVTVE"
     gene            complement(8874..9398)
                     /locus_tag="VFD06_09440"
     CDS             complement(8874..9398)
                     /locus_tag="VFD06_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03793.1"
                     /translation="MRGLLLVAAACALLLGAGTTLTAESPGNAALVVKVTPVTVVPGG
                     RAESKITIEIQKGYRIVAPGTALKWSQPVLLAFDAADGVFVEPPAWPAGTSWRGEEQD
                     PEVKVYEGRLDLRVTLRAAPKSAGQELALQGRLRYQAIKGEFFQKVAVMPVSIPVTVT
                     AAPAAPAGDAATRP"
     gene            complement(9404..10075)
                     /locus_tag="VFD06_09445"
     CDS             complement(9404..10075)
                     /locus_tag="VFD06_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03794.1"
                     /translation="MSRRSPVPPALTILVMAGAGVWLLPLGPSPRAQAVDPEPAIRWE
                     QAVSPGATVRIDIPPGWEVIEPVEPGDATRIRPLDGTPCDVRVEFVLASGAQPPVGSA
                     EALRAMVDGEARAFLDQAVESRYALRELQGPEAAGYYFMLRDRSPRRKGAVFLNRGAL
                     LVRDAVVRFSIETPKPDLPAIRQALKMLAGSRLQRVAPPTVPESAGAPAASTSAARTE
                     PGIGD"
     gene            10193..12424
                     /locus_tag="VFD06_09450"
     CDS             10193..12424
                     /locus_tag="VFD06_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZN03795.1"
                     /translation="METLLQSLNPRQREAVQHVEGPLLIVAGAGSGKTRVIVHRLAWI
                     LAHRLAAAHEVVAVTFTNKAAGEMKERVDELVGADHGGALVSTFHSYCLRLLRRYAPR
                     IGYGSDFLVYDEADQQTLVKDCFETLQIDEDSFPPRQMRARISDAKNRGLDPEGLRAE
                     ALGMRDELTAKVFALYQERLAAANAMDFDDLIGQVLRLFAEHPDTLGDVASRVRYLLV
                     DEYQDTNPPQYRLIRHLASVHGNVCAVGDPDQSIYKFRFADINNILSFESDFPGTRLI
                     KLEQNYRSTNNILGAATALVRHNRSRIDKELWSEAPAGEPIDLLVASDDRGEADRVVQ
                     EVRALRRAGDDLESMAALYRTNAQSRLLEEALTRAGIPYIMIGGTRFYDRKEVRDLLA
                     YLRLLINPKDDASLRRILNVPARDIGKTTLEAVLETARRDRLPLLEAVTAATEDRPGT
                     ERPVTTRAVKALAGFVDLMRDLREAARDLPPSRLVARIVSRTGFEQHLAATSPGDAQA
                     RIENLQELANAVAAYDGMEGGLTAFLDRTALLSETDNTQGSAGVRLMTLHSAKGLEFD
                     TVFIVGVEEDLCPHLRAAENEADLEEERRLLYVGMTRARRRLVLSRAGTRFQFGQART
                     TEPSRFLQEIPATLLRVRAEEAVERWARPDRPVRRFERTSATAIAARTGTFWTPDGDA
                     SRDEATPPSLYSLGCKVHHAEYGVGTVIGIEGSGDQQKVTVSFSIVGSKKFLPRFARL
                     ERL"
     gene            12421..13938
                     /locus_tag="VFD06_09455"
     CDS             12421..13938
                     /locus_tag="VFD06_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03796.1"
                     /translation="MRGSRAFLVRGLAAALVPVLAAATLRAEERSTRQLLQEGGLAIE
                     EGNWRVADSRFREAAEIDPRLAQAHYGIALAALGSLDRRGAEKALRTALTFAPNQPEL
                     RYTLGVVTYAFGDPRAAELDLKAAADADRRLLEARYAVGMAAGQRGDLAAAEAALREA
                     LKLDPQFAPAHFMLGAVLARAGNLDGSLQELSRGIARSPSVHEGRQEEAFDFAPRAIP
                     PSAASGTLGLPVPILRPSLQAARRRPGPAAPGTASEIPDWFLYYQMALQLEDSGQWAG
                     SVDMLQKALQSKERSEGLAVVANRLVDYSPHLHLASAYHHLDNFREAFLHLGIAKNEG
                     NASPDGLRALNVLVQKDRLRPRIALDPLPDRTGNEAITVRGLVVADDPVLRVEVSGRE
                     SQLRPLQASEIGDRMPELARSGSKDGVTGVAFEVKGYRLAEGSNLLTIRPFFRNPARD
                     GDVLEARIVRMPPKPGTKPQDKSSSPPPVAPKSPAPKPGEPPSQPGTLPPPGDGR"
     gene            13935..>14623
                     /locus_tag="VFD06_09460"
     CDS             13935..>14623
                     /locus_tag="VFD06_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03797.1"
                     /translation="MNRAPILETALLFSTSADAARARTRVRRGVALSMGVHGTLIAGL
                     VVLALTQIESVPPPPLTVRFFTLPPPPPRLVPLREQEPAPKPPPPPPPRQEPAVVPPL
                     HLAPPEPPPPAPPRPEPLPARLEPEAVPIRVADAAPEVRVHDSAPAVAAAPDLAPLGA
                     PAKPQIGTGEPEVAFLTPGDRQRRAGGTIAGRDQAPLPGAGSTTGGIRRGGSGLSTGG
                     DAAVRLREGRH"
BASE COUNT         2288 a         5286 c         4950 g         2099 t
ORIGIN      
        1 tgccggcgtc gaacccggtg ctgcggctcg gctcgaagga gtagatgcgc agctcatcgc
       61 tgttgaggat gttgaacacc tcgagcgaca ccccccaggt ggtcctcccc cagacgaagt
      121 tcttacgaac gctcccgtcg atgttgaggg tcgagccgtt gcgctcgctg ttgcgcgcga
      181 tggatcggaa cttcagccgc tccccgtcga gctccgtgaa gccgtaggtg gtccggaact
      241 gctggtaatc gacgttgtcg agggcgaaga agcgggacac gatcgagtac ggcagcccgc
      301 tggcccactg gctcgccaca ccgacctgcc agtcccccgg gaggaagatg ccggcgttca
      361 gcttcaccac gtggcgctgg tcgaagtcga gatagccgaa ctcgctctcg acgatggacg
      421 ggtcgttgcc gaggcgcgac tggaactcct ccgccgcgcc gatggcccgc gagtaggtgt
      481 acgacccctg catctgccag cggcgcgcca tccgccgccg cacctccacc tcgaaggcgt
      541 tgtagcgggc ggagttggtg ttccccaccc ggagcacctg gttgaagaag tagttgcgca
      601 cgaacaggtc gggccggccg tcgctgcccc gggtgatgac cgacggagcg tccggatcgt
      661 cggtggacac gacctcggtc gcgatcccga accgatcgct cagctcgccc gtgaaggggt
      721 cgacgcgggt ctcgtggttg acgtccacgt cctggagctg atccttgtag cggcgctgca
      781 ccgcccggac cgagagggcg gtctccgcgg cgatctcccg ctcgaagccg aggatccact
      841 cgtcgcagta gggggtcttg agatcccggt ccacctgggt cacggtcggc ggcgagatcg
      901 agacgatcgt cccgtaccca tgattgggga cgccgccggt gaccgcgccg gtctgcggga
      961 tgatggtcgg aacgaaagcg tcccggtcga ggacgtaata cctctggatt gtctccagcc
     1021 cctgctctcc cacgatggtg ttgaggaaca gccggtcgta gtagcgtccc cacgccccga
     1081 agaccttggt gcgtccgtcc ccctgcggat cccacgaggc cccgagacgg gggctgaggt
     1141 tgttgttggt gatccggaac gtctccggct gctggatctg gatcccgtag gaggccgcca
     1201 ctgcctggtc gaactgtcca tccttgacca ggcccgggaa gaaggcgttc agctgcgcca
     1261 ggctgaactc cgcgctgctg cggtgcaggg tcaggctcgc gtaggcggcg cgcttgaggc
     1321 cgttgacgat cggctcggtg accgtccccg tcgcggctcc tccgaagagc gggtcggacg
     1381 ggattccgcg gctctcgatc ccgtcgttgt tgccgacggt gaggtcgtcc tgccccgcct
     1441 cggcgccggc cagcgccagc aggcgatcga gacgctcgcg ctccgcctcc ggctcgaagg
     1501 aggtatagcc gctcgtggtg gcggcctccc ggtcgaagcg caatccgagc gacaggctca
     1561 cgttgggaat cggcttccag gtgtcctgga cgtagacccc tccgctgagc gactccgccg
     1621 actggtcgac actgggagtg gtcgggagga gggcggtgac gcggccgaac cccggatcga
     1681 tgcaggggtc gagataggga tcgcaatcga aatggacgcc cgggttgcgc acccggtcga
     1741 gcagattccc ggtgcgccag ccggggtcgt tgaccgcatt gaccggatgc ccgtcggccg
     1801 cgcgggcgaa ctgttccttc tcgaggaggt atccaccctt gatgtcgtgc gccccccgga
     1861 atccccccac gaacacgctg agatcctgac gcagggtggc gcgctcccgc tggtcctcgt
     1921 attcctgata gtagggtcct tcgatgacac ccttgcgcat gtcgatggag tagtcccgat
     1981 cccccggaac cggcgccagc cggccatacg gataaggccc ggtgggatac ggcgtgtcgt
     2041 cgatccggcc gtttctgttc cggtcctcag tcccgtcgtc gaggcgccgg tcgccgtcga
     2101 ggtcctctcc caaatcgagg ataccgttgc ggttgcggtc ctcgttccgg gcgtcgaggt
     2161 gggcatcgca gtcccggtcc tcgcggctga tcccctcgca gcctccgggg ggcgtcagac
     2221 gccggtcccc atcccctgga agcacctgcg gaaaaagcgg atcgatgcgc gctccgtcct
     2281 cgtccgagag gaacaggaga ggggcgctgc aggtgaaggg cggcgaatgc ccccagaagc
     2341 ccggccgttc cagcgtcggc gggatgaagc agaacaactc ttccaactgg tccagttggc
     2401 cgttgggcac gagcgtgtct tcccagacgt cgaacctgcc gtctccgtcg agatcctctc
     2461 ccggatcgcg ctcgcgcgcg tcacggaagc catcgccgtt gcggtcgatg tacaggatgc
     2521 cgtcgccgtt gccgtcgagc ggcaggttcg gcacgaccgc cggccggccg tcgaagtacg
     2581 acacggtcgt ctcgagggcc acggacgggc cgaggacgct ggtgccacgg gcgctgtaga
     2641 gcggccctcc ggccttcagc gcgtaccccg cctcctgccg cgtcaggctg ttcaacccct
     2701 ggttggtgaa ctcctgcggg tcgtagttca cgctgaatac cagccgcgtg ttcgcggtcg
     2761 cctgccacgt ccccttcgcg aacagacgct tttcctcgag cccggtcacg aatgcctgcg
     2821 tcaccgcgtt caccgggtct tccctctgga tgtactccag cgtcacgaag taccacgcct
     2881 tgtctttcac gatcggcccg ctcaacgaca ggaacggcag gtaatcgttg aaggtcaggt
     2941 cccgcagccc gacctccccg actcccccgt gcagggacga gtcgtcggtt cccgctccgt
     3001 cgccgtccag ggccgacccg cgccagtaga acttgaacgc tccctcgaag tcgttgccgc
     3061 ccgacttcgt caggatgttc gcgaagcccc cctgcgcccg gccgaactcc gccgacgccc
     3121 ccgccgtctt cacctcgatc tcctggatcg actcgatgtt cagctgcgcc cccaccttcc
     3181 cggtcagcgg gtcggtggtg ttgaccccgt ccacgaatgt ccccacgtcg gtgtcgcgcg
     3241 ctccgtggat gttgggattg ccgtccccgt cgacgtcgct cactccaggc gaaagcgtca
     3301 gcacctcctg gtagttccgt cccaggatcg gcaggttgtc gatgaactcc gaggagatgt
     3361 gggtggtcag gccgcgctct tcgaggctga ccgggctcgc cgtcccggtc actttgacct
     3421 gctcgcgcac gctcgactcc ggcagcagcg cgatcgtcac ggtcgtcatc tgaccggccc
     3481 gcaccgcgat gtccggcgtc gccacggtcg cgtatcccgg cagcgccgcc ttcaccacgt
     3541 agtcgtccga aggatcgagc gcgatcgcct ggaagcggcc ctcggcatcg ctccggccga
     3601 tcggccgatc gatctgcaga gtcctgctcg agacgctcag gagaacgctg atcagcggct
     3661 gcccggacag gtccacgacc cggccgcgga tgccgccgct cgtctgcgcc cggacgtgcc
     3721 ccaggcagac gaacagggcg agcgttcccc ccaacgcata gcgtttcaac acccacggct
     3781 ccagagcgag ttatgcggcg aaagcatgaa cgctacgcgc cgcgatccct cgggtcaacc
     3841 agttttcgtt cccgattcga gacggacgac cgaggatcgg tcggggcagc gcgcgagaag
     3901 ctccgcgatg gtgaggccga ggaccgggtg gacggcatcc ggggcgattt cagccagcgg
     3961 cacgaggacg aaccggcgca ggtggaggcg cgggtgcggc acctccaccc cctcctgctc
     4021 gaggaccacc tcccccaaaa gcaggagatc gaggtcgatt tcccggggtc ccttgtcctg
     4081 gcgcctcacc cggcccatcc cgcgctcgat gtcgaggcag atcgcgagga tgtcgggggc
     4141 ctcgagcggg gtccttacgg ctacgacctg gttgaggaat tccgcctggt cgaggaggtc
     4201 caccggctcc gtccggtaaa tcgaggagcg agcgacgacc cggagtccgg cctcctgcat
     4261 ccggtcgacg gcccgctcca gcagccgccg gcggttcccg acgttcgagc cgagcccgat
     4321 gcaggcggtc cggcccgcct cccccgaccg atccccccgc cgcttccccg tcatgccctc
     4381 tcccagccgg gacgccttga agaaccgggg cggcgcccga ccgcgcgttg acaccggacg
     4441 ataccatttg ctagcattca cggtttgcaa accattccat gaccttgggt tggactcccg
     4501 gccggatccc ttcgggagtc gtcgcggtgg cgcacatccc gccccggaat acggagaggc
     4561 atgaagaagc cgacgaagaa agtggcgaaa tccccggcgc cggcggtggc cggcggcaac
     4621 acctggaaca tccaggagaa ggcgctcaag gagatggagc gggggctgca ggcgatgcac
     4681 aagcagtcct gggccgacgc gcggacccac ttccagacca tcatcgacgg ctatcccaac
     4741 gagaaggagc tgcaggatcg ggcgcagatg tacgcgcggg tctgccgcaa ccacatcgag
     4801 agcgacggcg gatcgcagcg cgccaagccg gaggatctct tctacctcgg cgtcatgaag
     4861 tcgaacgagg ccgacttcga ccaggcggtc gagctcctgg accgggcgct gcagcacaac
     4921 ccgaaggacg agaggtcgca ctacgtcatg gccgcgaccc gggccctcaa gggggatcgc
     4981 gacgtcgccc tcaagcacct gcaggaggcg atcagcctca acgtccagaa ccgcttctac
     5041 gccatgaacg atccggattt cgagtcgatc cgggacgagg agaccttcga aaacctcgtc
     5101 taccccgaag aggcctgaac tccggaaggc gcccccggtg aaaaagaaga cgcggccgcg
     5161 gccgaaaggc gcgcgccgca gcaagcgctc gacgcagccc gccgtggtcc cggtcgcccc
     5221 gcccgcggcg acgcgccttc cccccgcccc gcctccgatc gcgacctcac tccgcccggc
     5281 cggggccagc gcggcgcacg cggcgattcc caacagcccc tatccgctgc gaccggtcgg
     5341 accggtgccg gaggaccgtc cacaggaggc gcgcggctcg aaccggcggg agaccctggc
     5401 cctcatcctc gcggccggcc tcggcaaacg catgcagtcg cggtggtcga agctgctcca
     5461 tcccgtcgcc ggccggcccg tggtgcgcca cgtggtcgag gcgacccgag gcgccggcgt
     5521 cgcccgcacc gtcgtcgtca tcggcaacca ggcggaggag gtgcggcggg cggtcggcga
     5581 tcacgacaag cggatcgcct tcgcgttcca gaaggagcag ctcggcaccg gacacgccgt
     5641 cctgtcggcg gagcggatga tgcacgggca cgatggggac gtgctcatcc tcaacggcga
     5701 tctgccggcc ctgcggcccg aaacgctggt cgccttcgtg gactaccacc gcgcgcacgc
     5761 cgccccgctg agcctgctca cgaccgtgct cgccgatccg cgcggctacg gccgcgtcat
     5821 ccgcaactac ggcggcgacg tgtcccgcat cgtcgaggac tccgacgcca gcgccgacga
     5881 gcgcgcgacc caggagatca actgcggcat ctactgcgcc gacagccggt cgctgttccg
     5941 gcccctgcgg caggcgaccc ggaacaacgc ccagggggag atctacctca ccgatctcat
     6001 cgagatcctg cgcaaggagg gtcgcaaggt cgccgcctac cggcatcccg aggcgggtga
     6061 ggtgctcggt gtgaacgacc ggcgcgagct ggccgaggcg ggacggtcgc tgtaccgccg
     6121 caaggcggag acgctgatgc gctccggcgt gacgctcatc gaccccgaca ccacctacat
     6181 cgacatgcag gcgaccgtcg ggcgcgacag catcatcgaa ccgggggtca tgatcctcgg
     6241 cgactcccat ctcggcgacg acgtccgcgt cgggctggga tcgcgcctcg tcgacacgac
     6301 gatcggcgac ggcaccgaga tcctggcgta ctgcgtcatc ggctcctcga aggtcggccg
     6361 cggctgccgt atcgggccgt tctcccatct ccggccggaa tcggcgctcg acgacgaggc
     6421 gcacgtcggc aacttcgtcg agacgaagaa gacccgcctc ggccgcggca gcaaggcgaa
     6481 ccacctctcc tacctcggcg acgccgagat cggccgcgac gccaacatcg gcgccggcac
     6541 catcacctgc aactacgacg gcgtcgccaa gcaccgcacc atcatcgagg acgaagtctt
     6601 catcggcagc gacacgcagc tcgtcgcccc ggtccgcgtc cgccgcggcg ccttcgtcgg
     6661 cgccggctcc acgatcacga aggacgtccc cgcgcacgcc ctcgccatca gccgggtccg
     6721 ccaggagaac aaagaaggct gggcgaagaa gcacggcccc ctcgcgaaac gagacggaca
     6781 caagtaaagc aggctctccg cacccccgga ggcgcaggac gcggagcgcc ctgcgccgca
     6841 cgcgcaaacc gcctgccttc cgcccatcgt ctcgatgcgt gtgttcgcct tccccgggca
     6901 tggcgcgccg cgtgccccgc gagggaccgc ggtttgcgcc tgaccaaggt cccggcgaac
     6961 acacgccgcc tcccctcgcc gtgggatggg ctccggggtg ccgcccttgt cgcttcgagc
     7021 acgtgaggct atatttccgc accccatgtg cggcatcgtt ggttacatcg gcgcgaagaa
     7081 agtcagcagc gtcatcctgg atgggctgcg gcggctggag taccgcggct acgactcggc
     7141 cgggatcgcg gtcatcgacg ggccgaacgt caaggtggtg cgggcggccg gcaagctccg
     7201 tgagctggag gagaacctcc ggctgacgca gctcgacggc tcgttcggcc tcgggcacac
     7261 gcgctgggcg acgcacgggc ggcccaccga ggagaacgcc catccgcacc gcgactgctc
     7321 cggctcgatg gtggtggtgc acaacggcat catcgagaac tacaaggagc tgaagaccca
     7381 gctcatcgga ctggggcacc agttcaagac cgagaccgac accgaggtga tcgcccacct
     7441 gatcgaagag gtgcgcaagg gcaagtcgga catgccgctc gaggacgggg tgcgcggcgc
     7501 cgtgccgcgc ctgaccggct cctacgcctt cgccgcggtt tccaccaagg acccgcgcaa
     7561 gatcgtcgcg gcgcgcaacg gcccgccgct ggtcatcgga ctgggcaagg acgagtactt
     7621 cgtcgcctcc gacatcccgg ccatcctgca ccacacccgc gacgtcttct tcctcgacga
     7681 ccgcgaggtg gcggtggtca ccgacggcgg cgtggtcatc accgaccacg acgggaagtc
     7741 gatccgcaag caggtgcagc ggatcacctg ggacccgatc ctggcggaga aggccggctt
     7801 caagcacttc atgctgaagg agatctacga gcagccgcgg gcgatccgcg acacgctcct
     7861 ggggcgcgtg tcgcaggaca ccgggaagat cttcctggac gagatggagg tcaccgagca
     7921 ggagttccgc gattgccgca agctccacat cgtcgcctgc ggcacctcct ggcacgccgc
     7981 gctcatcggc aagttcctga tcgagaagct cgcgaagctt ccggttgaag tggacatcgc
     8041 ctccgagttc cgctaccgct cgccgatcct cgagccggat cacctgctcc tggtgatcac
     8101 ccagtcgggc gagacggccg acacgctcgc cgcccagcgc gaggcgcgct cgcgtgggct
     8161 caagacgctc gccatctgca acgtcgtcgg ctcgatggcg acgcgcgagg cgcagggggt
     8221 catctacacc cacgccgggc cggagatcgg cgtcgcctcc accaaggcgt tcacggcgca
     8281 gatcgccgcc ctgtacctcc tggcgctgca cacggcgcag gtgcgcggcg cgctcacccc
     8341 ggaagagtgc aagtcgcaca tccgcgacct gctgctcatc ccgacgcaga tcgagcagat
     8401 cctcgccaag gacggcgaga tcgaggagat cgccaaggat ttccaccgcg cctccgactt
     8461 cctctacctc gggcgcggcg tgaactatcc gatcgcgctc gaaggggcgc tgaagctcaa
     8521 ggagatctcc tacatccacg ccgaggggta tccggccggc gagatgaagc acgggccgat
     8581 cgccctcatc gaccggaacc tgccggtcgt ggcgctggtc ttcggggacg ccctcttcga
     8641 gaagatgatg tcgaacgtcg aggaggcgca ggcgcgcgac ggcgccatca tcatggtgac
     8701 gcaggacggg cacgaggagg cgggccggcg ctgcgcccgc acggtgacga tcccgaagac
     8761 ctcggatctg ctgacgccga tcctcgcgtc gatcccgctg cagctgctcg cctaccacat
     8821 cgcgctgcgg cgcggctgcg acgtcgatca gccgcgcaac ctcgcgaagt cggtcacggt
     8881 cgagtagccg cgtctcccgc gggcgccgcc ggcgccgccg tgacggtgac cgggatcgac
     8941 accggcatca ccgccacctt ctggaagaac tcccccttga tcgcctggta gcgcagccgc
     9001 ccctggaggg cgagctcctg cccagccgac ttcggagcgg cgcgcagggt caccctgagg
     9061 tccagccggc cttcatacac cttcacctcc ggatcctgct cctcgccccg ccacgaggtc
     9121 ccggccggcc acgcgggcgg ctcgacgaag acgccgtcgg cggcgtcgaa cgcgaggagg
     9181 acgggctgcg accatttcaa ggcggtgccc ggcgcgacga tccggtatcc cttctggatc
     9241 tcgatcgtga tcttcgattc ggcccggccg ccggggacga cggtcaccgg cgtcaccttg
     9301 acgaccagcg cggcgttgcc ggggctttcg gcggtgaggg tcgttccggc gccgagcaga
     9361 agggcgcagg ccgccgcgac caggaggaga ccgcgcacga cggtcagtcc ccgattcccg
     9421 gctcggtgcg cgcggcggag gtcgaggccg cgggcgcgcc ggccgactcg ggcacggtgg
     9481 ggggagcaac ccgctggagc cggctgccgg ccagcatctt gagggcctgg cggatcgccg
     9541 gcagatccgg cttcggcgtc tcgatgctga accgcacgac ggcgtcccgc acgaggagcg
     9601 cgccgcgatt caggaagacc gcccctttgc gccgcgggga gcggtcgcgg agcatgaagt
     9661 aatagccggc agcctccggc ccctgcagct cgcgcagggc gtagcgggac tcgaccgcct
     9721 gatcgaggaa ggcgcgcgcc tcgccgtcca ccatcgcccg cagcgcctcg gcggacccga
     9781 ccgggggctg cgccccggag gcgaggacga actccacccg cacgtcgcac ggggtgccgt
     9841 cgagcggcct gatgcgggtg gcatcgcccg gctcgaccgg ctcgatcacc tcccacccgg
     9901 gcggaatgtc gatccggacc gtcgcgccgg gcgaaacggc ctgctcccag cggatggccg
     9961 gttcgggatc gaccgcctgc gcgcggggcg acggcccgag cggcagcagc cacacccccg
    10021 ccccggccat cacgaggatc gtgagcgcgg gcggaaccgg gctgcggcgc gacattggac
    10081 tggtctcgga caagcgagaa aactaccgcc agtcggggga tttcgtcaac ctgagagtcc
    10141 gggaaaagct cgggatggcg ccggaggccc ctgtgccata atttctgctc ccatggagac
    10201 tctgctccag agcctcaacc cgcggcagcg ggaggcggtc cagcacgtcg agggacccct
    10261 gttgatcgtc gccggcgccg gatccggcaa gacgcgggtc atcgtccaca ggctcgcctg
    10321 gatcctggcg caccgcctcg cggcggcgca cgaggtggtg gcggtcacct tcaccaacaa
    10381 ggcggctggc gagatgaagg agcgggtgga cgagctcgtc ggcgccgatc acggcggtgc
    10441 gctcgtctcc accttccact cgtactgcct gcgcctcctg cgccgctacg cgccgcgcat
    10501 cggctacggc tccgacttcc tggtctacga cgaggcggat cagcagacgc tggtgaagga
    10561 ctgcttcgag acgctccaga tcgacgagga ctcctttccg ccgcgccaga tgcgcgcccg
    10621 catcagcgac gcgaagaacc gcgggctcga tcccgagggg ctgcgcgccg aggcgctcgg
    10681 catgcgcgac gagctgacgg cgaaggtctt cgccctgtac caggagcgcc tcgccgcggc
    10741 caacgcgatg gacttcgacg atctgatcgg ccaggtgctg cggctgttcg cggagcaccc
    10801 ggacaccctg ggcgacgtcg cctcgcgggt gcgctacctc ctggtggacg agtaccagga
    10861 caccaacccg ccgcagtacc ggctgatccg ccacctcgcg tcggtgcacg gcaacgtctg
    10921 cgccgtcggc gacccggatc agtcgatcta caagttccgc ttcgccgaca tcaacaacat
    10981 cctgtcgttc gagagcgatt tcccgggcac gcgcctcatc aagctggagc agaactaccg
    11041 ctcgacgaac aacatcctcg gcgccgccac ggcgctcgtg cgccacaacc gcagccgcat
    11101 cgacaaggag ctgtggagcg aagcccctgc gggggagccg atcgatctcc tggtcgcctc
    11161 cgacgatcgc ggcgaggcgg atcgcgtggt ccaggaggtc cgggcgctgc gccgcgccgg
    11221 ggacgacctg gagtcgatgg cggcgctgta ccggaccaac gcgcaatcgc gtctcctcga
    11281 ggaggcgctc acccgcgccg ggatcccgta catcatgatc ggcggcaccc gcttctacga
    11341 tcgcaaggag gtgcgcgacc tcctcgccta cctgcgcctg ctgatcaatc ccaaggacga
    11401 cgcgagcctg cggcgcatcc tcaacgtacc ggcgcgcgac atcggcaaga cgacgctcga
    11461 ggcggtgctg gagacggcgc gacgcgaccg cctgccgctc ctcgaggcgg tgacggcggc
    11521 gaccgaggac cggccgggga cggagcgccc ggtcacgacg cgcgcggtca aggcgctcgc
    11581 cggcttcgtc gacctgatgc gcgacctgcg cgaggcggcg cgcgacctgc ccccgagccg
    11641 gctggtggcg cgcatcgtca gccgcaccgg cttcgagcag cacctggcgg cgacctcccc
    11701 gggagatgcc caggcgcgca tcgagaacct gcaggagctg gcgaacgcgg tcgccgccta
    11761 cgacggcatg gaggggggcc tgaccgcctt cctcgatcgc accgcgcttc tgtccgagac
    11821 cgacaacacg caggggtccg cgggggtgcg gctgatgacc ctgcactcgg ccaaggggct
    11881 ggaattcgac accgtcttca tcgtcggcgt cgaagaggat ctctgcccgc acctgcgcgc
    11941 cgccgagaac gaggccgacc tcgaggagga gcggcgtctc ctctacgtcg gcatgacgcg
    12001 ggcccgcagg cggcttgtcc tcagccgggc cggaacccgc ttccagttcg gccaggcgcg
    12061 cacgaccgaa ccctcccgtt tcctccagga gatccccgcg acgctcttgc gcgtgagggc
    12121 ggaggaggcg gtggagcgct gggcccggcc ggatcgcccg gtgcgccgct tcgagcggac
    12181 ctccgcgacc gcgatcgccg cccgcaccgg caccttctgg acgccggacg gcgacgcctc
    12241 gcgcgacgag gcgaccccgc caagcctcta ctcgctcggt tgcaaggtgc accacgccga
    12301 atacggcgtc gggacggtga tcggcatcga ggggagcggc gaccagcaga aggtgacggt
    12361 gtcgttctcg atcgtcggct cgaagaagtt cctgccccgc ttcgcccggc tggagcgcct
    12421 gtgagggggt cccgtgcctt cctcgtccgg ggcctggccg cggccctcgt gccggtgctc
    12481 gccgcggcaa cgctccgggc cgaggagcgc tcgacgcgcc aactcctgca ggaagggggc
    12541 ctggcgatcg aagaggggaa ctggcgcgtc gccgacagcc gtttccgcga ggcggccgag
    12601 atcgatcccc gactcgcgca ggcgcattac ggcatcgccc tcgcggcgct cggaagcctc
    12661 gaccggcgcg gcgccgagaa ggcgctgcgc accgcgctga ccttcgcccc caaccagccg
    12721 gagctgcgct acaccctggg ggtcgtcacc tacgccttcg gcgatccgcg cgcggcggag
    12781 ctcgacctca aggcggcggc cgacgccgat cggcgcctgc tcgaggcgcg ctacgccgtc
    12841 gggatggcgg cgggccagcg cggcgacctc gcggcggccg aggccgccct gcgggaggcg
    12901 ctgaagctcg acccgcagtt cgccccggcg catttcatgc tgggagcggt gctcgcccgc
    12961 gccggcaatc tcgacggatc cctccaggag ctgtcgcgtg gcatcgcgcg ctcgccgtcg
    13021 gtccacgagg ggcgccagga ggaggccttc gacttcgccc cgcgcgccat tccgccgtcc
    13081 gccgcgtccg ggacgctggg gcttccggtg ccgatcctcc ggccgtcgct gcaggcggcg
    13141 cgccggcgcc ccggcccggc cgctcccggc accgcctccg agatcccgga ctggttcctc
    13201 tattaccaga tggcgctgca gctcgaggac tccgggcagt gggccggctc ggtcgacatg
    13261 ctgcagaagg ccctgcagtc gaaggagcga tccgaggggc tggcggtcgt cgcgaaccgg
    13321 ctggtcgact actcgccgca cctgcacctg gcgagcgcct atcaccacct cgacaacttc
    13381 cgcgaggcct tcctgcacct cggcatcgcc aagaacgaag gaaacgcctc ccccgacggg
    13441 ctgcgggctc tcaacgtcct ggtgcagaag gatcgcctgc ggccgcgcat cgccctcgat
    13501 ccccttccgg acaggaccgg caacgaggcg atcaccgtgc gcggcctggt cgtcgccgac
    13561 gacccggtgc tgcgggtcga ggtgtcgggg cgcgagtcgc agctccgccc gttgcaggcg
    13621 tcggagatcg gcgaccgtat gccggagctg gcacgctccg gctcgaagga cggtgtcacc
    13681 ggcgtcgcct tcgaggtgaa ggggtaccgc ctcgccgagg gcagcaacct cctgacgatc
    13741 cgccccttct tccgcaaccc ggcgcgcgac ggggacgtgc tcgaggcgcg catcgtgcgg
    13801 atgccgccga agccggggac gaaaccgcag gacaagagct ccagcccccc gccggtggcg
    13861 ccgaagtccc ccgcgccgaa gccgggggag ccgccttcgc agccggggac gctccctcca
    13921 ccgggagacg gacggtgaac cgggctccga tcctcgagac agcgctcctg ttcagcacga
    13981 gcgcggacgc ggccagggca cgcacacgcg tccggcgcgg cgtggcgttg tcgatggggg
    14041 tgcacgggac gctgatcgcc ggcctcgtcg tcctggccct gacccagatc gaatcggttc
    14101 ctccgccgcc gctcacggtc cgtttcttca cgctgccgcc tccgccgccg cgcctcgtgc
    14161 cgctgcggga gcaggagccg gccccgaagc cgcctccccc gccaccgccg cgccaggagc
    14221 cggccgtcgt gccgccgctg catctggccc cgcccgagcc gccaccgccg gcgccgcccc
    14281 ggccggagcc gctgccggcg cgtctcgagc cggaagcggt cccgatccgg gtcgccgacg
    14341 ccgcccccga ggtgcgcgtg cacgacagcg cccccgcggt cgcggcggcg ccggatctcg
    14401 ccccgctcgg cgcaccggcg aagccgcaga tcggcaccgg cgagccggaa gtggcgttcc
    14461 tgactcccgg cgaccgccag cgccgcgccg gcggcacgat cgccggccgg gaccaggccc
    14521 cgctgcctgg cgccggctcc accaccggag gaatccggcg cggcggcagc gggctctcca
    14581 cgggcggcga cgccgcggta cgcctccgag agggacggca cca
//
LOCUS       DASRIQ010000388         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1468876, whole genome shotgun sequence.
ACCESSION   DASRIQ010000388 DASRIQ010000000
VERSION     DASRIQ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468876"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFD06_09465"
     CDS             <1..550
                     /locus_tag="VFD06_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03798.1"
                     /translation="NLAGTAAATGFNHGSGPLSQAQDPNPSLGAVIYYQAATNSPGGA
                     NLDAYGCPSPSICSNPGWCDLGTSAGGPCTTNGDCPGGGTCVLEPTFCLTDAGTAKQG
                     GCGHHPTCVGGSAPGKLCQSAVDCPGGGACPAATAAQQATEGQVCLTLTGAPLAAAPY
                     GNCPPAGHPKRVVSRTGGLACP"
     gene            670..3249
                     /locus_tag="VFD06_09470"
     CDS             670..3249
                     /locus_tag="VFD06_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HZN03799.1"
                     /translation="MRPLDPEEVLRHYRRGRGLLAIRGKIPIRDEYMMSLVYGPGVGA
                     AVQEIQENPAASFSLTMRGNAVGIVTDGSSVLSYGDAGPAAALPVMEGKAVLFKALAG
                     LDAFPLCLDERDPRRIAQIIRRLMPTFGSFAIEDIASPRSFELQEILEGMSDTDLPVP
                     YFFNDMQGASAIVLAALRNALRIVNKEIGAIKAVITGAGGAGISAARFLRRAGLQDIT
                     LCDRHGIVRAGRPEGMNRFREAAAREFNPSGVEGSVRDALRGADLFVGLSAARTVGHD
                     MIRAMAPRAIVFALATPEPEIDPDEARDAGATVVLTGGANYRHGLNVALAFPGILRGV
                     MDARAPRVYDEMLIAAADAIAALVPEKEISPDRIVPRVLDLRVGPAVARAVANAAVAL
                     GVAGNDVHPDEIGEQTRHLVYEGDTALVEHPAPRGDRGLPRSLGDEALDLHRRYRGAL
                     AVAPKLPLKDELMLGVIATPGVAVPVREILADPMNVWEYTTKGNLVAVVTDGTSVPTL
                     GPVGALAALPMIEGKCVLLKTFAGIEAMPLSIEAREPVEVVTLVRALSPALGGVLLEG
                     IATPACFEIERLLRQSTDIPIFHDAQHGTAVVVLAALMNAARLTDREPERMRVVINGA
                     GAGGAAVARLLLAAGIQDIVLCDAKGALFEGRPGMTPEKAALAQRTNPERLQGSLAQV
                     VRGRDLFVGLSRGGLLTSDAVRSMAERPIIFALAWPIPEIAPDAALAAGASVVATSRH
                     ERPNPISNLLAFPGILRGALDVAARDIDDGMLLAAADALAGLIPDYELTASSILPRPL
                     EFQGVPEVAMAVARAALKSGLARRRVDPHEVLTRTRDYLYGGTLLSIPDQRGALAEVP
                     PKA"
     gene            3370..>3891
                     /locus_tag="VFD06_09475"
     CDS             3370..>3891
                     /locus_tag="VFD06_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HZN03800.1"
                     /translation="MATAATKEVLNPFEIATKQFDTAASYLKLEPWLHDVLKAPKRQL
                     IVSIPTKMDDGSIHVFEGYRVQHSMARGPAKGGIRYHPDVTLDEVKALASWMTWKCAT
                     VNIPYGGGKGGVVCDPKKMSQRELENMTRRFAFEISVIIGPERDIPAPDVYTNAQTMA
                     WIMDTYSMTAGETV"
BASE COUNT          561 a         1411 c         1405 g          514 t
ORIGIN      
        1 gaacctggcg gggaccgcgg cggcgaccgg gttcaaccac ggctcggggc cgctgtcgca
       61 ggcgcaggat cccaatcctt ccctcggcgc tgtcatctat taccaggcgg cgacgaacag
      121 cccgggcgga gccaacctgg acgcctacgg ctgtccgagc ccgtcgatct gcagcaaccc
      181 gggatggtgc gacctgggaa cgagcgcagg ggggccgtgc acgacgaacg gcgactgtcc
      241 cggcggcggg acctgcgtcc tcgagccgac cttctgcttg acggacgccg ggaccgcgaa
      301 gcagggaggc tgcggtcatc acccgacctg cgtgggagga agcgctcccg ggaaactgtg
      361 ccagtcggcg gtcgattgcc ccggaggggg cgcttgcccc gcggcgacgg cggcgcagca
      421 ggcgaccgaa ggacaggtct gcctgacgct gaccggggcg ccgctggcgg cggcccccta
      481 cggcaactgc ccgccggcgg ggcatccgaa gagggtcgtg agccgcaccg gagggctcgc
      541 ctgcccgtag gcgctccggc gccggccggc cctgccccgc cggcgctgct tcgcgaaaaa
      601 accggccgcc gcgcgcttga cctcggcgcg cggcggcccg taacatgaag tcactttggc
      661 gaaaggttcg tgaggccgct cgaccccgaa gaagtcctgc gccactaccg ccgcggccgc
      721 ggtctcctcg ccatccgcgg caagattccg attcgcgacg agtacatgat gagcctggtg
      781 tacggcccgg gggtcggggc cgccgtccag gagatccagg agaaccccgc cgcctccttc
      841 agcctcacga tgcgcggcaa cgccgtcggc atcgtcaccg acgggtcgtc ggtgctgtcg
      901 tacggcgacg ccggccccgc cgcggcgctg ccggtgatgg aggggaaggc ggtcctgttc
      961 aaggcgctcg ccgggctcga cgccttcccg ctctgtctcg acgagcgtga cccgaggcgg
     1021 atcgcgcaga tcatccggcg gctgatgcca acctttgggt ccttcgccat cgaggacatc
     1081 gcctcgcccc gctccttcga gctgcaggag atcctcgagg ggatgagcga caccgatctg
     1141 ccggtaccgt acttcttcaa cgacatgcag ggggcgtcgg cgatcgtcct cgcggcgctg
     1201 cgcaacgccc tgcgcatcgt caacaaggag atcggcgcca tcaaggcggt gatcaccggc
     1261 gccggcggcg ccggcatcag cgccgcccga ttcctgcgcc gggccgggct ccaggacatc
     1321 accctgtgcg accggcacgg catcgtgcgc gccggccggc ccgaggggat gaaccgcttc
     1381 agggaggcgg cggcgcgcga gttcaacccg agcggggtcg agggctcggt gcgcgacgcg
     1441 ctgcgcggcg ccgacctctt cgtcgggctg tcggcggcgc gcaccgtcgg ccacgacatg
     1501 atccgcgcca tggcgccgcg cgccatcgtc ttcgccctcg cgacgccgga gccggagatc
     1561 gatccggacg aggcgcgcga cgccggcgcg acggtggtcc tcaccggcgg cgccaactac
     1621 cggcacggcc tcaacgtcgc cctggcgttc cccggcatcc tgcgcggggt catggacgcg
     1681 cgcgccccgc gcgtctacga cgagatgctc atcgccgcgg ccgacgccat cgcggcgctc
     1741 gtgccggaga aggagatctc ccccgaccgg atcgtgcccc gcgtcctgga tctgagggtc
     1801 gggccggcgg tggcccgggc ggtggcgaac gccgcggtcg cgctcggagt cgccggcaac
     1861 gacgtgcacc cggacgagat cggcgagcag acgcgccacc tggtctacga gggggacacg
     1921 gcgctcgtcg agcacccggc cccccgcggc gaccgcgggc tgccccgctc gctcggcgac
     1981 gaggcactcg acctgcaccg gcgctaccgc ggcgcgctgg cggtcgcccc caagctgccg
     2041 ctcaaggacg agctgatgct gggggtcatc gccacgccgg gggtcgccgt gccggtgcgc
     2101 gagatcctcg ccgacccgat gaacgtctgg gagtacacca ccaaggggaa cctcgtcgcg
     2161 gtcgtcaccg acggcacctc ggtgccgacc ctggggccgg tcggggcgct ggcggcgctg
     2221 ccgatgatcg aggggaagtg cgtcctcctg aagaccttcg ccgggatcga ggcgatgccg
     2281 ctgtcgatcg aggcgcgcga gccggtggag gtcgtgaccc tggtgcgggc gttgtcgccg
     2341 gcgctgggag gggtgctcct cgaggggatc gcgacgccgg cctgcttcga gatcgagcgg
     2401 ctgctccggc agtccaccga catcccgatc ttccacgacg cgcagcacgg caccgccgtc
     2461 gtcgtcctcg cggcgctcat gaacgccgcg cggctcaccg accgcgagcc ggaacggatg
     2521 cgcgtcgtga tcaacggcgc cggggccggc ggggcggcgg tggcgcggct gcttctggcc
     2581 gccggcatcc aggacatcgt cctgtgcgac gccaaggggg cgctgttcga aggacggccg
     2641 gggatgaccc cggagaaggc ggcgctggcg cagcgcacca atcccgagag gctgcagggg
     2701 agcctggccc aggtggtgcg cggacgcgac ctcttcgtcg ggctgtcgcg cggcgggctg
     2761 ctcacctccg acgcggtccg cagcatggcg gagcgcccga tcatcttcgc cctcgcctgg
     2821 ccgatcccgg agatcgcccc ggacgcggcg ctggccgccg gcgcgtcggt cgtcgccacc
     2881 tcgcgccacg agcggccgaa cccgatcagc aacctcctcg ccttccctgg gatcctgcgc
     2941 ggggcgctcg acgtcgcggc gcgcgacatc gacgacggga tgctgctggc ggcggcggac
     3001 gcgctcgccg ggctcatccc cgactacgag ctgaccgcgt cgagcatcct gccgcggccg
     3061 ctcgagttcc agggcgtgcc ggaggtggcc atggcggtgg cgcgcgccgc cctcaagtcc
     3121 ggcctcgccc gccggcgggt cgacccgcac gaggtcctca cacgcacccg tgactatctc
     3181 tacggcggga ccctcctctc catcccggac cagcgggggg ccctcgccga ggtccccccc
     3241 aaggcctagg ccccgggggc cgaaccccgc gcccggcggg ggcttccggt gagggtcgct
     3301 ttacgctccc cggacgctgt ggtatgaagg aggccccctc agagacggac gacacggagg
     3361 atgcggacga tggccacggc ggcgaccaag gaagtcctca atccgttcga gatcgcgacg
     3421 aagcagttcg acaccgcggc ctcctacctg aagctcgagc cctggctgca cgatgtcctc
     3481 aaggccccca agcgccagct gatcgtctcc atcccgacga agatggacga cgggtcgatc
     3541 cacgtcttcg aggggtaccg cgtgcagcac agcatggcgc gcgggccggc caaggggggc
     3601 atccgctatc accccgacgt cacgctcgac gaggtgaagg cgctcgcctc ctggatgacc
     3661 tggaagtgcg ccacggtgaa catcccgtac ggcggcggca aggggggggt cgtctgcgac
     3721 cccaagaaga tgtcgcagcg cgagctggag aacatgaccc gccgcttcgc cttcgagatc
     3781 agcgtcatca tcgggccgga gcgcgacatc cccgcccccg acgtctacac caacgcgcag
     3841 acgatggcgt ggatcatgga cacgtattcg atgacggcgg gggagacggt g
//
LOCUS       DASRIQ010000389        11055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_163500, whole genome shotgun sequence.
ACCESSION   DASRIQ010000389 DASRIQ010000000
VERSION     DASRIQ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163500"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VFD06_09480"
     CDS             <1..871
                     /locus_tag="VFD06_09480"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZN03801.1"
                     /translation="HVEGGLLGPCATGVTSCAGGPMECVQTVFPSAETCNGVDDDCDG
                     STDEDQPPGGEEVCNGLDDDCDDVVDEGLGTLSCGTGACARTVDACVGGSTQTCVPGI
                     PSEEACNGLDDDCDGQTDEGLGSLSCGTGVCARTVAACSGGSSQTCVPGAPAPEACNG
                     LDDDCDGQTDEEAAWSGLLQPVNQDGSSIFQQKSTIPLKFRLTKCSGEGITTATATLE
                     VVPISNTILGTVDEGTLPNARADIGDVFRFDLKGNQFVYNLASKSLIPGRSYLLRIRI
                     NGGPAHETVISLK"
     gene            948..4583
                     /locus_tag="VFD06_09485"
     CDS             948..4583
                     /locus_tag="VFD06_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03802.1"
                     /translation="MVCPACGRDNLSGAGRCSACGRLLPETSAGTADRAKEEEPRGAS
                     SPPSEAATSLHGTPARGGTNVTSLTDANAAGGPGVPRLAPGTLLGSRYQIETVLGEGG
                     MGVVYRARDRELNRTVALKVIRPELTSHPEILERFKREILLASQVTHKNVVRIHDLGE
                     AGDLRFLSMSYIEGESLKDLLDREGPLPPERGVPLLRGIATALQAAHDAGVVHRDLKP
                     HNVLVDKDGQPYVGDFGISRSMTSDGTMTETGAILGTVDYMSPEQACGDVPDHRSDIY
                     ALGVMMFEMFTGSLPFRASNPLSVMVKRVHEDAPSPTRVRPGMPPWLSAIILRALMRD
                     PKLRYQSVTELLRDVDRQRASRAKRRLLGKRAAAAAVGVVIAGLLILGAQRFLATRVS
                     APPPVKSSLALLPFRNETGDARLDWVPAGLTSVLRSGLLQARALRLAGDDRVSEILDV
                     LKPSPGEEARPATAQRLGRLAGVDHVLAGSVVRFGDRLRLQASLIPIGEGRAGEPRAI
                     VVDGEPGRGADGGQEALLGMIDRLTREVREALGVARAWGEEERLASQLSSKSVEALSL
                     YGEGLSLRQSGRANEAAERLEAAVKKDPDFAVAWALLAETEDERGRAEPARAAAAQAV
                     AHLSGASPWEAARIRAVQARLTGDLAGAAVSFQSIVDAAPNDPQSLLDLANVQEEAGD
                     LAGARRSLERTVTLDPRNPGAQFALGRVLTRSGQSSDAAQAMNRALLLYSELGSDAGR
                     AQSINGLGVVALDLGQHEEAETQFRQALEIRKRIGDERGVAVGLGNLALTAARRGRVN
                     DAVRLENESIAAARDLGDPGVEAMGLLNLGDILQEAGRTEEALGAYQESLRILREAGD
                     ENQTAKVLSSLGYLNGVLGKYVEAFFFMKEALAKARAIGEQTNLVRTLSDIGALESVQ
                     GRYDEALKYFDEGLRLARQVDNKGSAIVLQMNTAQVHEAQGEYGAALKLLAEAEKSAR
                     DARETSYLAPVLTYLGSARQRSGDLAGSAAALKEAIALCREQDNDALLAEALVYDGER
                     LLAQGDRGASLALRDAVAAAERAGDYRVQRMARLARARAEGSRRDLETVLRDARTSGL
                     APLVAPALVSTAAIDLQAGRLDEAIARAREAADAAAKLKERDWIVQARRLEGSALLRQ
                     GKKEPAAAAFRLALAPFEEIRAGLSGPALAAFLARPETAALGRDATPALASAPADRDR
                     LQALLRP"
     gene            4720..6774
                     /locus_tag="VFD06_09490"
     CDS             4720..6774
                     /locus_tag="VFD06_09490"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HZN03803.1"
                     /translation="MHRSKAALFLGAMGAVLLATMPAAADCTLYTTEAAFLDALDGGT
                     TRETFDGFDSGTEINDQIPGLFIDAPGESSAAVVIASEDAVSQPNVLSGGVPGPSGIF
                     LPQALQLDFDPSIGAIAFYLTNLDPLGSAAGVTLSFDDEGSQNASIEDSDDDATTPEF
                     FGAICTSAINSALVRSGSFAGGEAGVDVFGIDDLIRASGDLGDCCDPTCDGNPLVVDG
                     ILGISGTGRDSSEFGSGIESIVLQEGAENVALTVDEFEAGDLAVDFRTGPIDPLLDGQ
                     GTVLVTDVAGKTCSLPVTFRAIDPGPVSGELICSGGGTLLSVTNLLAGPGGPAACSVE
                     APGDGDPALPPGYEPSPEDDPFPCTVMTIQSPISGLTDMTYKKDGDFEPRLRLLFSHF
                     DGAAFPPFTDISESVDQITTVTPDPTRVKGSGGWSQVKVTCAVLGEICNGADDDGDGS
                     IDEGLPVGGAAVDADGDGVPLCPASAGGAFDCNDQIAAIHPGAGEACNGMDDDCDSGV
                     DEGQPADGEACSIPGLLGVCAEGETSCEAGPLVCAQVNLPSDEVCDGADNDCDGTADE
                     GYVFGGYLQPVNPDGSSIFRRGNAVPFKFRLTDCGGAVVSTATARIHVFFYSNGVVGT
                     EVEDVGSVGSANTDNQYRYDPTGQQYIYNLSTLILRSNTTYLVRTTLDDGTEHDVLIS
                     IR"
     gene            complement(6789..8090)
                     /locus_tag="VFD06_09495"
     CDS             complement(6789..8090)
                     /locus_tag="VFD06_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN03804.1"
                     /translation="MALEPTLFPKGAYGSLEERTFIAKSASGSLIEDVDGNHYIDFGS
                     GWGTNNVGNCHPEVVEAVNGMMRQAGVTCWTSAANSVQRIALAEKLLAVCPKGHDRVV
                     FLTTGTEAVEGALRLMRRGTGRPYVISFYGQYHGLSYGALKAGPLNADMRDDLALLGS
                     GFLHAPYPYTYRTPLRSQKGGGAGQATLEYIEEMLLFYDVPPRQIAGVLVEPVVGEAG
                     VWIPPDDFLPGLRAMCDKYEWALCIDEVQSGFGRCGRMWALEHWNVVPDLMVIGKGLS
                     GGSMPIAAVSGKADLMDRAEAFISSTYSGHPAACAAAIKTIEILERDDVLRHASELGV
                     YGLDRLRAMQEKYRMIGEVRGLGLWLAAEFVKDRGTRERDYEAEMEVNAHCLQNGLYY
                     IHDSITWFARLQPPLTIERDLFAQGIDILEDAIAAVDAKRR"
     gene            complement(8173..9615)
                     /locus_tag="VFD06_09500"
     CDS             complement(8173..9615)
                     /locus_tag="VFD06_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN03805.1"
                     /translation="MSSRVRGGAARCARTRPARGMALAAAAALLAAATLPAAGCRGSA
                     RPNIVLLIADTVRADRFSWGGAPRVVAPRLEAMARDATVYAQAFSPAPWTVPAHASLF
                     TGRYPSAHRTDCGSLKLPDAEATLAEALHEAGYRTVGYTANPWLGATYNFQQGFDTWG
                     ETWREVPEGSPDTGAGLNNQRIERFLRWYAGNPDARRQPFFLFANYFEAHLPYHPPAP
                     ERARMLRSGADPARVERLSRLGHPEEMAFILGRSDLTDADLAVLNDLYDGEVAYVDRR
                     LGEVLDVLQETGLLDHTVVVVASDHGENLGEHGMLDHKMSVHATLLRVPLLVRYPRAV
                     PAGSRIDAPVQLHDLFPTLLALAGIPLPREGVVEAVLLPGITGGRPRDSNQAIVGEFA
                     GPPVEFLQVMARAFPGADLSRFDRTLVSLRREGYTLHWGSDGRHALYRNADEPGESVD
                     LASSEPERVRALANEVDVWLRRPARRGEAR"
     gene            9681..>11055
                     /locus_tag="VFD06_09505"
     CDS             9681..>11055
                     /locus_tag="VFD06_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HZN03806.1"
                     /translation="MPSTPSDPVRSGSTPLIHGPLPGPKAKAAIERDRRVVSQNLSKD
                     VPLVVARGEGMTIEDVDGNRFLDFAAGISTVSTGHCHPDVVAAVKEQAERLLHVCYTD
                     FYYPIYIELCETLARLAPISGPKRIFLTNSGAEAVETAIKLARVRTGRQKVIGFFGAF
                     HGRTFGAMSLTASKPVQRKGYAPFVPEVYHTHYAYCYRCPVNRKPESCSVECLELLTE
                     QHFHHTIDPKEVAAVIVEPVQGEGGFIPPHPQFLPRLQAICREHGILLIADEVQSGMG
                     RTGKMFALEHYGIDPDMIILAKGIASGMPISAVIAREDVMRWNDGGHGSTFGGNPVSC
                     AAALATLRLLEGGLVANAGKVGARLMEGLRGLQAKHPMIGDVRGLGLMVGVEIVRDRG
                     TRAPDPETRHAMIVETFERGLLILPCGASTLRLSPPLVCTEKDADTAVAILDAACAAV
                     AAALPQAG"
BASE COUNT         1664 a         3932 c         3917 g         1542 t
ORIGIN      
        1 ccatgtcgag gggggactcc tgggtccctg tgccacgggg gtgacctcgt gcgcgggcgg
       61 gccgatggaa tgcgtgcaga ccgtcttccc gtcggcggag acctgcaacg gcgtcgacga
      121 cgactgcgac ggctcgaccg acgaggacca acctcccggc ggtgaggagg tgtgcaacgg
      181 tctcgacgac gactgcgacg acgtggtcga cgaggggctc ggcaccctgt cctgcggcac
      241 cggagcttgc gcccgcactg tcgacgcctg cgtcggcgga tcgacgcaga cctgcgtccc
      301 cggcatcccg tccgaggagg cctgcaacgg tctcgacgac gactgtgacg gacagaccga
      361 cgaggggctc ggcagtctct cctgcggcac cggcgtctgc gcccgcaccg tcgccgcctg
      421 ctccggcgga tcgtcgcaga cctgcgtccc cggcgcgccc gccccggagg cctgcaacgg
      481 tctcgacgac gactgcgacg ggcagaccga cgaggaggcg gcctggagcg gtctgctgca
      541 gccggtcaac caggacggct ccagcatctt ccagcagaag agcaccatcc cgctcaagtt
      601 ccggctgacc aaatgctcgg gcgaggggat cacgaccgcc accgcgaccc tcgaggtcgt
      661 gccgatttcg aacaccattc tcggcacggt cgacgaaggg acgctgccga acgcccgggc
      721 cgacatcggc gacgtgttcc ggttcgacct caaggggaat cagttcgttt acaacctcgc
      781 gtccaaatcc ctgattccgg gcaggtcgta tctcctgcgg atccggatca acggcggccc
      841 tgctcacgag acggtgatct cgctgaaata gggcccgggc caggagtctg tccgagtctg
      901 cggacgcccc gcgccgccga ggctcccccc gtctagaatg acgggtcatg gtgtgcccgg
      961 cctgcggtcg cgacaatctt tcgggagccg ggaggtgctc cgcctgcggg cgtctgctcc
     1021 cggagacttc tgccggcacg gccgaccggg cgaaggagga ggagccccgc ggcgcctcca
     1081 gcccgccctc tgaagcagcc accagcctcc acggtacgcc ggctcgaggc ggcaccaacg
     1141 tcaccagcct gaccgacgcc aacgcggccg gcggacccgg cgtcccccgg ctcgcccccg
     1201 ggacactcct cggcagccgc taccagatcg agacggtcct cggcgagggg gggatggggg
     1261 tcgtgtaccg ggcgcgcgac cgcgagctca accgcaccgt cgcgctcaag gtcatccgtc
     1321 ccgagctgac ctcgcaccca gagatcctcg agcgcttcaa gcgggagatc ctgctcgcga
     1381 gccaggtcac ccacaagaac gtcgtccgca tccacgacct cggcgaggcc ggcgacctgc
     1441 gcttcctgtc gatgtcctac atcgaggggg aaagcctcaa ggacctcctc gatcgcgagg
     1501 ggccgctgcc gccggagcgc ggcgtcccgc tcctgcgcgg catcgccacg gcgctgcagg
     1561 cggcgcacga cgccggcgtc gtgcaccgcg acctcaagcc gcacaacgtc ctggtcgaca
     1621 aggacggtca gccgtacgtc ggggacttcg ggatctcccg ctcgatgacc agcgacggga
     1681 cgatgaccga gaccggcgcc atcctcggca cggtcgacta catgtcgccg gagcaggcgt
     1741 gcggcgacgt gccggatcac cgcagcgaca tctacgcgct cggcgtgatg atgttcgaga
     1801 tgttcaccgg cagcctcccc ttccgtgcct ccaacccgct gtcggtgatg gtcaagcggg
     1861 tgcacgaaga cgcgccgtcg cccacgcgcg tccggcccgg gatgccgccc tggctgtcgg
     1921 cgatcatcct gcgcgccctg atgcgcgacc cgaagctgcg ctaccagagc gtcaccgagc
     1981 tcctgcgcga cgtcgatcga cagcgggcga gccgggccaa gcggcgcctg ctcgggaagc
     2041 gagcggcggc cgcggcggtc ggggtcgtca tcgccgggct gctcatcctg ggggcgcagc
     2101 gcttcctcgc gacgcgggtc tccgcgcctc ccccggtgaa atcgtccctc gccctcctgc
     2161 cgttccgcaa cgagacgggg gatgcgcgcc tcgactgggt cccggccggg ctgacgagcg
     2221 tcctgcggtc ggggctcctg caggcgcgcg ccctgcgcct cgccggcgac gaccgggtca
     2281 gcgagatcct ggacgtgctg aagccctcgc cgggggagga ggcgcgcccc gccacggcgc
     2341 agcggctcgg ccggctcgcc ggcgtcgacc acgtgctcgc gggcagcgtc gtgcgcttcg
     2401 gcgatcgcct ccgcctgcag gcgagcctca tcccgatcgg cgaaggaagg gcgggcgagc
     2461 cgcgcgccat cgtcgtcgac ggcgagcccg gccgcggggc ggacggcggg caggaggcgc
     2521 tcctcggcat gatcgaccgc ctcacgcgcg aggtgcgcga ggcgctcggc gtggcgcgcg
     2581 cctgggggga ggaggagcgt ctcgcgtcgc agctctcctc gaagtcggtc gaggcgctgt
     2641 cgctgtacgg cgaaggcctg tcgctgcgcc agtccggccg ggccaacgag gcggcggagc
     2701 gcctcgaggc ggccgtgaag aaggacccgg acttcgccgt cgcctgggcg cttctggcgg
     2761 agaccgagga cgagcgcggg cgcgccgagc cggcgcgcgc cgcggcggct caggcggtcg
     2821 cgcacctgag cggcgcctcg ccgtgggagg cggcgcgcat ccgcgcggtc caagcccggc
     2881 tcaccgggga tctggccggc gcggcggtct cgttccagtc gatcgtcgac gccgccccca
     2941 acgatccgca gtcgctcctc gacctcgcca acgtgcagga ggaggcaggg gatctcgccg
     3001 gggcgcgccg gtcgctcgag cgcaccgtga ccctcgatcc gcgcaatccg ggcgcgcagt
     3061 tcgccctcgg ccgggtcctg acccggtcgg gacagtcgag cgacgcagcg caggcgatga
     3121 accgggcgct cctcctgtac agcgagctcg gtagcgacgc cgggcgcgcg cagtcgatca
     3181 acgggctcgg cgtcgtcgcg ctcgatctcg ggcagcacga ggaggcggag acccagttcc
     3241 ggcaggcgct cgagatccgc aagcgcatcg gcgacgagcg tggcgtcgcg gtggggctcg
     3301 gcaacctcgc gctcacggcg gcgcgccggg gacgcgtgaa cgacgcggtc cggctcgaga
     3361 acgagtcgat cgccgcggcc cgcgatctcg gcgatccggg ggtcgaggcg atggggctcc
     3421 tgaatctcgg ggacatcctg caggaggccg gccgcaccga ggaggcgctc ggcgcctacc
     3481 aggagagcct gcgcatcctg cgcgaggccg gcgacgagaa tcagaccgcc aaggtgctga
     3541 gcagcctcgg gtacctgaac ggagtcctcg gcaagtacgt cgaggccttc ttcttcatga
     3601 aggaggcgct cgccaaggcg cgggcgatcg gcgagcagac gaacctggtg cgcacgctgt
     3661 ccgacatcgg cgccctcgag tcggtgcagg ggcgctacga cgaggcgctg aagtacttcg
     3721 acgaagggct gcggctggcc cggcaggtgg acaacaaggg gagcgcgatc gtgctccaga
     3781 tgaacaccgc ccaggtccac gaggcccagg gggagtacgg cgcggcgctc aagctcctcg
     3841 ccgaggccga gaagtcggcg cgcgacgcga gggagacctc ctacctcgcc ccggtcctga
     3901 cctacctcgg cagtgcgcgg cagcgatccg gcgacctcgc gggctccgcc gcggcgttga
     3961 aggaggcgat cgcgctgtgc cgggagcagg acaacgatgc cctcctcgcc gaggcgctgg
     4021 tgtacgacgg tgagcggctc ctggcgcagg gagaccgcgg cgcctccctg gccctcaggg
     4081 acgcggtcgc ggccgcggag cgggcgggcg actaccgggt gcagcgcatg gcgcggctgg
     4141 cgcgggcgcg cgccgaggga tcccggcgcg atctggagac cgtgctcagg gatgcgcgca
     4201 cctccggcct cgcgccgctc gtcgcgcccg cgctcgtgtc gacggcggcg atcgatctgc
     4261 aggcggggcg gctggacgag gcgatcgccc gggcgcggga ggcggccgac gcggccgcga
     4321 agctcaagga gcgcgactgg atcgtgcagg cgcgccgtct cgaagggagc gcgctcctcc
     4381 ggcaggggaa gaaggagccg gcggcggcag cgttccgcct cgcgctcgcg ccattcgagg
     4441 agatccgggc cgggctctcc ggcccggcgc tcgccgcgtt cctggcgcgc ccggagaccg
     4501 ccgcgctcgg acgcgacgca acgcccgccc tggcgtccgc cccggccgat cgcgatcgac
     4561 tgcaggcgct cctgcgcccc tgatcgtccc tcccgatcgc ggacggcgac cgcatcgccg
     4621 gacgccgcgt ctccgttgcg ttccgccact cctgaaaatc ctttgacagt gccacttttc
     4681 cgcgcctaga atccggaaaa acatacgaag ggggattcca tgcaccgctc gaaggccgcc
     4741 ctgttcctgg gagcgatggg tgccgtgctg ctcgcgacca tgccggccgc ggccgactgc
     4801 actctgtaca ccaccgaggc cgcgttcctc gacgcgctcg acggcggcac gacccgcgag
     4861 acgttcgatg gcttcgacag tggcaccgaa atcaacgacc agatccccgg gctcttcatc
     4921 gatgctcccg gagagagctc cgccgcggtg gtcatcgcgt ccgaggatgc agtgagccag
     4981 ccgaacgtgc tctcaggagg cgtccccggc ccgtccggca tctttctgcc gcaggccctg
     5041 cagctcgact tcgatccctc gatcggggcg atcgccttct acctcacgaa cctggacccg
     5101 ctggggtcgg ccgccggagt gacgctctcc ttcgatgacg aagggtcgca gaacgcgtcg
     5161 atcgaggact ccgacgatga tgcgacgacg ccggaatttt tcggcgccat ctgcacgtcg
     5221 gccatcaact cggccctcgt ccgctcgggc tcgttcgccg gaggcgaggc gggggtcgac
     5281 gtcttcggca tcgacgatct gatccgggcg tcgggggacc tcggcgactg ctgcgacccg
     5341 acctgcgacg gcaacccgct cgtggtcgac gggatcctcg gcatcagtgg caccggccgc
     5401 gattcgagcg agttcggcag cggcatcgag tcgatcgtcc tgcaggaggg ggccgagaac
     5461 gtcgccctga ccgtggacga gttcgaggcc ggcgatctcg ccgtggactt ccggaccggg
     5521 ccgatcgacc cgttgctcga cggacagggg accgtgctgg tcaccgacgt cgcgggaaag
     5581 acctgttccc tgcctgtcac gttccgcgcc atcgatccgg gaccggtgtc cggcgaattg
     5641 atctgcagcg gtggcggaac actcctgagc gtcaccaatc tcctcgcggg accgggagga
     5701 ccggcggcgt gcagcgtgga ggctccgggc gacggcgatc cggctcttcc gccgggctac
     5761 gagccctccc ccgaagacga tccgttcccc tgcacggtca tgaccatcca gtcgccgatt
     5821 tccggcctca ccgacatgac gtacaagaag gacggggact tcgagccgcg gctgcggctc
     5881 ctcttctcac acttcgacgg cgcggccttc ccgccgttca ccgacatctc cgagtcggtg
     5941 gaccagatca cgacggtcac gccggacccg acacgcgtga aggggagcgg agggtggtcc
     6001 caggtgaagg tcacgtgcgc ggtcctcggc gagatctgca acggcgccga cgacgacggc
     6061 gacggctcca tcgacgaggg gctgccggtg ggcggcgcag cggtggacgc cgacggcgac
     6121 ggcgtgccgc tctgcccggc gtccgccggg ggcgccttcg actgcaacga ccagatcgcc
     6181 gccatccatc cgggcgccgg cgaggcgtgc aacggcatgg acgacgactg cgacagcggg
     6241 gtcgacgagg gccagccggc ggacggggag gcctgctcca tccccggtct gctgggcgtc
     6301 tgcgccgaag gggaaacctc ctgcgaagcc ggaccgctgg tctgcgcgca ggtgaacctc
     6361 ccgtcggacg aggtgtgtga cggcgcggac aacgactgcg acgggacggc ggacgaggga
     6421 tacgtcttcg gcggctacct gcaaccggtc aatcccgacg gcagcagcat cttccgccgc
     6481 ggcaatgccg tgccgttcaa gttccggctc acggactgcg gcggcgccgt cgtgtccacg
     6541 gcaacggcga ggatccacgt cttcttctac agcaacggcg tggtcggcac cgaagtcgag
     6601 gacgtcggct cggtcggcag cgcgaacacc gacaaccagt atcggtacga cccgaccggc
     6661 cagcagtaca tctacaacct gagcaccctg atcctgcggt ccaacaccac gtacctggtc
     6721 cgcacgaccc tcgacgacgg caccgagcat gacgtgctga tatcgattcg ctgagctgac
     6781 ggccgccgtc agcgacgctt cgcgtcgacc gcggcgatgg cgtcttcgag gatgtcgatg
     6841 ccctgagcaa acaggtcgcg ctcgatcgtc agcggcggct gaaggcgggc gaaccaggtg
     6901 atgctgtcgt ggatgtagta gaggccgttc tggaggcagt gggcgttcac ttccatctcg
     6961 gcctcgtagt cgcgctcgcg ggtgccgcga tccttgacga actcggcggc gagccagagg
     7021 cccaggcccc ggacttcgcc gatcatccgg tatttctcct gcatcgcgcg caggcgatcg
     7081 agcccgtaca ccccgagctc gctggcgtgc cgcaggacgt catcgcgctc caggatctcg
     7141 atcgtcttga tggcggcggc gcaggcggcc ggatggccgg agtaggtgct ggagatgaac
     7201 gcctccgccc ggtccatgag gtccgccttc ccggagacgg cggcgatcgg catcgaacct
     7261 cccgagagcc ccttgccgat caccatgaga tccgggacca cgttccagtg ctcgagcgcc
     7321 cacatccgcc cgcagcgtcc gaagccgctc tgcacctcgt cgatgcagag cgcccattcg
     7381 tacttgtcgc acatcgcccg cagccccggg aggaagtcgt ccggcgggat ccagacgccg
     7441 gcctccccca ccaccggctc gaccagcacc ccggcgatct gccgcggcgg gacgtcgtag
     7501 aacagcagca tctcctcgat gtactcgagc gtcgcctgcc ccgccccgcc ccccttctgg
     7561 gagcgcagcg gggtccggta ggtgtaggga tacggcgcgt gcaggaagcc cgacccgagg
     7621 agcgccaggt cgtcgcgcat gtcggcgttg agcggccccg ccttcagcgc cccgtacgac
     7681 aggccgtggt actggccgta gaacgagatc acgtaggggc ggccggtgcc acggcgcatc
     7741 aggcgcagcg ccccctcgac cgcctcggtg ccggtggtca ggaagacgac gcggtcgtgc
     7801 cccttcgggc agaccgccag aagcttctcg gcgagcgcga tccgctgcac gctgttggcg
     7861 gcggaggtcc agcaggtcac acctgcctgc cgcatcatcc cgttgaccgc ctcgacgacc
     7921 tcgggatgac agttgccgac gttgttcgtt ccccagcccg acccgaagtc gatgtagtgg
     7981 ttgccgtcga cgtcctcgat gagcgacccc gaggcgctct tcgcgatgaa ggtgcgctct
     8041 tcgagcgacc cgtaagcccc cttcgggaag agcgtcggct cgagcgccat gaggcggcgg
     8101 ctctccggcc ccggaggcgg ggttttgatc agtgaaacgc gtttcggtgg gggcatgggg
     8161 tgattatagg tctcaccggg cctctcccct ccgagccggc cgcctcagcc agacatcgac
     8221 ctcgttcgcc agggcgcgca cgcgttccgg ctccgaggac gccagatcca ccgactcgcc
     8281 gggctcgtcg gcgttccggt acagggcgtg acggccgtcc gagccccagt ggagcgtgta
     8341 cccctcgcgt cgcaacgaca cgagggtgcg gtcgaagcgg gacaggtcgg cgccgggaaa
     8401 cgcccgcgcc atgacctgga ggaactcgac cggggggccg gcgaactccc cgacgatcgc
     8461 ctggttcgaa tcgcgcgggc gcccgccggt gatgccgggg agcagcaccg cctcgacgac
     8521 tccctcccgc ggcaggggga tcccggcgag ggcgagaagc gtcggaaaga gatcgtgcag
     8581 ctgcaccggc gcgtcgatcc ggctcccggc cgggacagcg cgcgggtagc gcaccaggag
     8641 cggcacgcgg aggagcgtcg cgtgcacgct catcttgtga tcgagcatcc cgtgctcgcc
     8701 gaggttctcg ccgtgatccg acgccaccac gacgaccgtg tggtcgagca ggccggtctc
     8761 ctggagcacg tcgagcacct cgccgaggcg tcgatcgacg tacgccacct cgccgtcgta
     8821 gagatcgttc agcaccgcga ggtcggcgtc cgtcaggtcg gaacggccga ggatgaaggc
     8881 catctcctcc ggatggccga ggcgcgacag gcgctccacg cgcgccggat cggcgccgga
     8941 ccggagcatc cgggcgcgct cgggcgccgg cgggtggtac ggcaggtgcg cctcgaagta
     9001 gttcgcgaac aggaagaacg gctggcggcg agcgtccggg ttgccggcat accagcgcag
     9061 gaagcgctcg atccgctggt tgttgagccc ggcaccggtg tcgggggatc cctccggcac
     9121 ctcgcgccac gtctcccccc aggtgtcgaa tccctgctgg aagttgtaag tcgccccgag
     9181 ccacgggttg gccgtgtacc cgacggtgcg gtagccggcc tcgtgcagcg cctcggcgag
     9241 cgtcgcctcc gcgtcgggaa gcttcagcga gccgcagtcg gtgcggtgcg ccgacggata
     9301 ccggccggtg aacaggctgg catgcgccgg caccgtccag ggggcgggcg agaaggcctg
     9361 cgcgtagacc gtggcgtcgc gcgccatcgc ctcgaggcgc ggcgcgacca cgcgcggcgc
     9421 gcccccccag gagaagcgat cggcccgcac ggtgtcggcg atcagcagca cgatgttcgg
     9481 tcgcgccgat ccgcggcacc cggccgccgg gagcgtcgcg gccgccagga gcgcggccgc
     9541 cgccgcgagc gccatcccgc gcgccggacg cgtccgcgcg cagcgcgcgg cgccgccgcg
     9601 gacgcggctt gacacctccg gacgcgctcc ccgacaatga cgactcacga tcaggaaccc
     9661 tgtttcaagc ggagaaaccg atgccttcga cgccctccga ccccgtccgc tccggctcga
     9721 cgccgctcat tcacggcccg ctccccggcc cgaaggcgaa ggccgccatc gagcgcgacc
     9781 gtcgcgtcgt gtcgcagaac ctcagcaagg acgtgccgct ggtcgtggcg cgcggcgagg
     9841 ggatgacgat cgaggacgtc gacggcaacc gcttcctcga cttcgccgcc ggcatctcga
     9901 ccgtctcgac gggtcactgc caccctgacg tcgtcgcggc ggtgaaggag caggcggagc
     9961 ggctcctgca tgtctgttac acggacttct actacccgat ctacatcgag ctgtgcgaga
    10021 cgctggcgcg cctcgcgccg atctccggcc cgaagcgcat cttcctgacc aattccggcg
    10081 ccgaggcggt cgagaccgcg atcaagctgg cgcgcgtgcg caccggacgc cagaaagtga
    10141 tcgggttctt cggagcgttc cacggccgca ccttcggcgc catgtcgctc accgccagca
    10201 agccggtgca gcgcaaaggg tacgcgccgt tcgtgccgga ggtgtatcac acgcactacg
    10261 cctactgcta ccgctgtccg gtcaaccgca agccggagtc gtgctcggtc gagtgcctcg
    10321 agctgctcac cgagcagcac ttccaccaca ccatcgatcc gaaggaggtg gcggcggtga
    10381 tcgtcgaacc ggtccagggg gaggggggct tcatcccgcc gcacccgcag ttcctgccgc
    10441 gcctgcaggc gatatgccgc gagcacggca tcctgctcat cgccgacgag gtgcagagcg
    10501 gcatggggcg gaccggaaag atgttcgcgc tcgagcacta cggcatcgac ccggacatga
    10561 tcatcctcgc caaggggatc gcctccggca tgccgatctc ggcggtgatc gcgcgggagg
    10621 acgtgatgag gtggaacgac ggcggtcacg gaagcacgtt tgggggcaac ccggtgtcct
    10681 gcgcggcggc cctcgcgacg ctgcggctgc tcgaaggggg gctcgtggcc aacgccggga
    10741 aggtcggcgc gcgcctcatg gaagggctgc gcggcctgca ggcgaagcac ccgatgatcg
    10801 gcgacgtgcg cggcctcgga ctgatggtcg gcgtcgagat cgtccgcgac cgggggacgc
    10861 gcgcgcccga cccggagacg cgccacgcga tgatcgtgga gacgttcgaa cgcggcctcc
    10921 tgatcctgcc gtgcggcgcc tccaccctgc gcctgtcgcc gccgctcgtc tgcaccgaga
    10981 aggacgccga caccgcggtg gcgatcctgg atgccgcgtg cgccgccgtg gccgcggccc
    11041 tccctcaggc gggcg
//
LOCUS       DASRIQ010000390         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2233079, whole genome shotgun sequence.
ACCESSION   DASRIQ010000390 DASRIQ010000000
VERSION     DASRIQ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233079"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFD06_09510"
     CDS             complement(<1..245)
                     /locus_tag="VFD06_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03807.1"
                     /translation="MAIRTTTFALGLGCAVVTLILRGDASPAVDQGIAGGVTVHQDDA
                     GGRWVSSAEVQASVPGELIVKYRETVLLPVDRLLSRRT"
     gene            complement(489..902)
                     /locus_tag="VFD06_09515"
     CDS             complement(489..902)
                     /locus_tag="VFD06_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HZN03808.1"
                     /translation="MRPTLRVGIGIVVALALVWTTLIIALVVLRPKDGLLREAMRILP
                     DTIRLLRRLATDRSIGVGVRVRLWLLFAYLASPVDLIPDFVPVLGYADDAIIVAAVLR
                     SVVRRAGPDAIRRNWPGTPDGLSTVWTLAGLDGAS"
     gene            complement(899..1102)
                     /locus_tag="VFD06_09520"
     CDS             complement(899..1102)
                     /locus_tag="VFD06_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN03809.1"
                     /translation="MLTQRRAQATGKSLAEVRQELMAGQSIKEFVNPRDIAALAVFLA
                     SDAARSISGQMLPIDNDIQTTGG"
     gene            1466..1708
                     /locus_tag="VFD06_09525"
     CDS             1466..1708
                     /locus_tag="VFD06_09525"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HZN03810.1"
                     /translation="MAKLTLNVDESVVARAKRYAARRGTSVSRLVEQLLALVIGSARE
                     DDEDVPPVLARLRDELKGSAADPAAYRRYLERKYDR"
     gene            1705..2226
                     /locus_tag="VFD06_09530"
     CDS             1705..2226
                     /locus_tag="VFD06_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZN03811.1"
                     /translation="MRRTLVDVNVLLDAVLERKPHAAAAARIWAAVETGRITGLVPAH
                     AVTTLFYLFAKARGGSAARRSIGQLLTVFGVAPVNLPVLQRALILGWTDFEDAVCAAA
                     AEGAGCTLIVTRDAAGFRSSPVRAVDPAGALALLEQESGPGRLSEPRRTAYVTRRTAR
                     PQRRRRPRSSPRP"
     gene            2363..>2745
                     /locus_tag="VFD06_09535"
     CDS             2363..>2745
                     /locus_tag="VFD06_09535"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN03812.1"
                     /translation="MKPLLCTRRSSAPPDRLWAIASDFARAPERIKAITKVEMLTPGP
                     VGVGTRFREWRGQNVVDMEIAAWSPPRSDSLRACAIGTEFMSEIRCVPDGAGTRLEME
                     VHVRPRTFGAKLLTPLLALTSRMMV"
BASE COUNT          474 a          926 c          931 g          414 t
ORIGIN      
        1 gtacgccggg agagcaggcg gtcgaccggc agcaggacgg tctcccggta cttcacgatc
       61 agctcgccgg gcacggaggc ctgcacctcg gcggaagaaa cccaccggcc gcccgcgtcg
      121 tcctgatgga cggtgacacc cccggcgatc ccctggtcga cggccggaga ggcgtccccg
      181 cgcaggatca gagtgaccac cgcgcaaccg agaccgagcg cgaacgtcgt cgtgcggatc
      241 gccatcgaag cacctccccg cagtgggtgc ctctatagtg ttcggtggcg gcgaaaggtc
      301 gcgaaaaaat cgacccggaa ctcgggcgtc gtcccgaatg cctgagccag gagccctgac
      361 gcctattcgc acgcgttgtc gtccttgccg ttgcacaggc cgccctgggt gagcgcgatc
      421 tggccttcaa tgggctcgac caggcttatg aggattcccg gatgacggac gcggcaacgt
      481 cttgctactc acgatgcgcc gtcgagcccc gcgagagtcc acacggttga gagcccatcg
      541 ggcgttccgg gccaattccg gcgaatcgca tcaggtccgg cccgccggac gacggaacgg
      601 aggacggcgg caacgatgat ggcgtcgtcc gcgtagccca ggacgggcac gaagtctgga
      661 atgaggtcca cgggacttgc gaggtaagcg aagaggagcc acagacggac gcggactccg
      721 accccgattg acctgtcggt cgccagccgg cggagcaagc gaatcgtgtc cggcaggatc
      781 cgcatcgcct cacgcagcag cccgtccttg ggtcgcaaga caacgagcgc gatgatgagc
      841 gtcgtccaga ccaacgccaa ggcgacgacg atgccgatcc caacccgtag cgtcggcctc
      901 acccgcctgt cgtttggatg tcgttgtcga tcggcagcat ctggccggag atcgatcggg
      961 ccgcatccga agccaggaag acggcgagcg cagcgatgtc ccgggggttc acgaattcct
     1021 tgatcgactg gccggccatc agctcctggc ggacttcggc gagcgacttg ccggtcgcct
     1081 gggcccggcg ttgggtgaga atttcatata cgcactttca ggagtgaccg caaccagttc
     1141 tttgtggcgc gggaaaatgc ccgacaagcg tgcgggccgc cggctcagaa gatggccggg
     1201 ctccgggccg cccagtcctg gcgccaggag agaagttctt ccggtcggag cggtcggaac
     1261 cgccggcggc gccgggctgc tccgggatcc gggggcactc cggcacgacg gcgatgtccg
     1321 cctcgagcgt cgccagatcg gagagcttgc ggtccagagg tcccgagcag cagttccagg
     1381 tgacgaggcg caccaagccg gatcctatgc ccgattcggg cctcaaggga ttgcatacgt
     1441 acagaatgcc atctaccata cgtacatggc caagctgacc ctgaatgtgg atgagagcgt
     1501 cgtggcccgc gccaagcgct atgcggcacg gcgcgggacc tccgtgtcgc ggctggtcga
     1561 gcagctcctg gccctggtga tcggaagcgc acgcgaggat gacgaggatg tcccgccggt
     1621 cctggcgcgt ctgcgggacg agctgaaagg ctcggccgcc gatcctgccg cataccgccg
     1681 atacctggag cgcaagtacg accgatgagg cgcactctgg tcgacgtcaa cgtccttctc
     1741 gacgccgtgc tggaacggaa gcctcatgcg gcggctgcgg cgaggatctg ggcggccgtc
     1801 gagacgggac gaatcaccgg tctcgtgccc gcccacgcgg tcacgactct tttctatctg
     1861 ttcgccaagg cgcgcggagg ctccgccgcg cgccgcagca tcggacaatt gctcaccgtc
     1921 ttcggagtgg cgcccgtcaa tctgccggtc cttcagcgcg cgctgatcct gggctggacc
     1981 gacttcgagg acgccgtctg cgcggccgcc gccgagggcg ccgggtgcac cttgatcgtg
     2041 acgcgggacg cggcggggtt ccgctcctcg ccggtacgcg ccgtcgatcc ggcaggcgcc
     2101 ctggcgctgc tggagcagga aagcggaccc ggtcggctct ccgagcctcg ccggaccgcc
     2161 tacgtcaccc gtcggacagc gcggccacaa agacggcgca gaccacgcag ctctccgcga
     2221 ccctgaacct gcgatcgccg aagcgctcaa gaggctggag atcgagacgc tcttcgcgcc
     2281 gggcaactga ggatcgcagg cattcccgag ggtgtcgcag gaaccaggat gacccaacgg
     2341 catccaagag gaggaccgcc cgatgaaacc gctgctctgt acgcgccgtt cgtccgcacc
     2401 gcccgaccgc ctgtgggcga ttgcgtcgga cttcgcgcgc gcccccgagc gcatcaaggc
     2461 gatcacgaag gtcgagatgc tgacgcccgg tcccgtcggc gtcggcactc gcttccgcga
     2521 gtggcgcggc cagaacgtcg tggacatgga gatcgccgcg tggtcgccac cgcgcagcga
     2581 ctcgctccga gcctgcgcca tcgggaccga gttcatgtcg gaaatccgct gcgtccccga
     2641 cggcgccggc acccgcctgg agatggaggt tcacgtgcgc ccccggacct tcggcgcgaa
     2701 gctcctcacc ccgcttctcg cgctgacgtc aaggatgatg gtgaa
//
LOCUS       DASRIQ010000391         7251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2484129, whole genome shotgun sequence.
ACCESSION   DASRIQ010000391 DASRIQ010000000
VERSION     DASRIQ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484129"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFD06_09540"
     CDS             complement(<1..265)
                     /locus_tag="VFD06_09540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044308.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZN03813.1"
                     /translation="MPVEHPAADDSLGGEEMVLNMGPQHPSTHGVLRLVVTLQGETVK
                     SIVPHVGYLHRGMEKIAEGMTYNQFIPYTDRMDYLAPLSTNVGY"
     gene            complement(331..873)
                     /locus_tag="VFD06_09545"
     CDS             complement(331..873)
                     /locus_tag="VFD06_09545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZN03814.1"
                     /translation="MKGPYIEPNPPRHVVDLLRARFPEAGIGAEEFRGEITVTLPAER
                     LVDAATFLRDDAETSFDMPIACVAVHFLEGEFEHDVMWQVYSLEKNHRLRLKLRLRPG
                     ETAPSLTSIWAGMNWPEREQYDLVGVRFTGHPDLRRIIMAEDYPDHPLRKDFDVEGGP
                     AAIDGEGRPASPGWRDMEHA"
     gene            complement(881..1234)
                     /locus_tag="VFD06_09550"
     CDS             complement(881..1234)
                     /locus_tag="VFD06_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZN03815.1"
                     /translation="MFDYIPIGLQAVLALILAGVILTLATLLGRPKRSRTDLSPYECG
                     VDPLDASNKPVSVRFYMVAMLFILFDIEAAFLYPWAVVLRDMGMRGFAGMFVFLLVLV
                     VGYVYCWRRGALEWD"
     gene            1405..1836
                     /gene="arfB"
                     /locus_tag="VFD06_09555"
     CDS             1405..1836
                     /gene="arfB"
                     /locus_tag="VFD06_09555"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HZN03816.1"
                     /translation="MTAVPPRTPSAPADLPPESEVEISAIRAQGKGGQNVNKVSNAVH
                     LRFDIRASSLPEEIKERLLGMRDRRISAEGVVVIKAQTERSLEQNREAALGRLRAMVA
                     AAAAVPRTRRPTRATRGSKERRVEGKKRRAHVKALRRRVEE"
     gene            1884..3710
                     /locus_tag="VFD06_09560"
     CDS             1884..3710
                     /locus_tag="VFD06_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN03817.1"
                     /translation="MRRSPGFRRLVPILFLSAVPVIHAAAVAPAKPGAGDLAAARAVF
                     EKNLDAIRRRDQEAYLSCYLKSPTFVRTGADGPMKGYDSLAKEPPEWPDAFEGLALDL
                     VPLRPGLVYGTYRYRVRYGGREESGISERFFVKTDAGWKIAVSTAFAAPPGTPPPPRA
                     LTGATLIDATSAAPVADSVVVLRGGKIDCAGTRAQCPIPEGVERLDLKGMWVTPGLVD
                     AHMHHAQTGWADGRPDFIDVRDKHPYETLQAGLRAHPERWWRSYLCSGVTAVFDVGGY
                     PWSWDLRSRAGADPLAPHVAAAGPLLSTLDFWLNLPAERQFIYLGTEKDARSGVTYLA
                     DHGTDAVKVWFIPVASRNFDEMAQAVKAAGQEAHTREVPLIVHATGLKEAVAALKAGA
                     DLLVHSVEDAPVNDEFLRLAKSNGTIYCPTLTVIGGYRRLQEAAAAHQVPAVDDPNGC
                     TDAETTKNLAETATLTMKPDDPGVAERRRQRFETFAKMTAANLKTVRDAGIPIAMGTD
                     AGNPLTLHGVSVYAEMEAMQAAGLTAMEVLVASTRNGARAMRRDADFGTVEKGKVADL
                     LVLGADPSKDIRAMRQIRFVVRGGVVRAQSELRPEPRPPQEH"
     gene            complement(3707..4858)
                     /locus_tag="VFD06_09565"
     CDS             complement(3707..4858)
                     /locus_tag="VFD06_09565"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HZN03818.1"
                     /translation="MNERAIASPQSRPDPAARPTSATERVHALDAIRGAAILGVLLAY
                     TVWSLGNPPEAEWSRLDRLVERLMGLLVDNRFLTLFAFLFGVGVAQQWRRWEAAGADV
                     RRLHLRRMGFLGGLGLLHAVFLRNGDILLPYALLGLALLLFRRGSGPLLVGCIVVFLL
                     LPTLFEWAVPRLGLAWPARPSAAGRPYVLENLEWLRHWFVTNPLRSWPRVIGLMLAGV
                     LAGRARLFERLAIDRPLQRRFLAVAAAVAGVMTLALVVWQGRSDCAPASLECLGRDLL
                     AHATAWAVASAYLALAFFVLGAGTPDRLSWLRAVGRMAFTNYVLQAVIAVPICLAFGL
                     FDRISPTRGVLAALLIGLGQAAFSVWWLRSHSMGPLERLWRRFTYGAPA"
     gene            complement(4897..5340)
                     /locus_tag="VFD06_09570"
     CDS             complement(4897..5340)
                     /locus_tag="VFD06_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN03819.1"
                     /translation="MNLFRRILVPHDFSRHATRALEYAAGLAKAGGGRLIVLHAIPPF
                     QPITAHMIAARTYLPEGEEVRAARGRLEGLVARTVRGARPRVEVRVVIDDPLQAIDEA
                     SRGADLIVMSTAGRTGLARLLIGSIAEKVVRHARVPVLTIRPRTR"
     gene            5424..6446
                     /gene="gap"
                     /locus_tag="VFD06_09575"
     CDS             5424..6446
                     /gene="gap"
                     /locus_tag="VFD06_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZN03820.1"
                     /translation="MTVRVGINGFGRIGRLFLRAAKSRDLPFNFVAINDLADVDQMAL
                     LLRYDSIHGPYPGKVEAEDDDLIVDGDRIRTHKVTDKREGDPFPFPWGEVGADVVVEA
                     TGVFRRIAELQRHIAAGAKKVVLTVPAKDPLEATVVMGVNDHILKASDRIVSNSSCTT
                     NCAAPVAKVLQDLYGIREGFLTTVHAYTNDQRLFDHPHKDVRRGRMAPMNIVPTSTGA
                     AKALGLVIPALKGKLEGLAFRVPVPSGSVVDLTVITEKRPAVDEIHAAMKAAAEGPMK
                     GILQVTEDPIVSADVIGNPHSSIYDSLLTQVPHEDMVKIVAWYDNEWGYAMRLVDLVE
                     KVAALG"
     gene            complement(6496..6762)
                     /locus_tag="VFD06_09580"
     CDS             complement(6496..6762)
                     /locus_tag="VFD06_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03821.1"
                     /translation="MRKTPIRPVQHFSDEYLEHCKAMKSDEVLDFLESFRRLHAEASS
                     GSRLISLKVPQDLLNAFRWRCDLAGVRYQTQIKRLMRDWLGIET"
     gene            complement(6759..7220)
                     /locus_tag="VFD06_09585"
     CDS             complement(6759..7220)
                     /locus_tag="VFD06_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03822.1"
                     /translation="MFILRVAEAFDRARLPYAIVGGYAVALHGAVRGTVDIDLVIRLR
                     AADFRKTETLFLDLGLRPRLPVAASEVFQFREEYIRNRNMRAWTFVNPARPSEIVDVI
                     LTEDLAHMKTRRIAVRGGILRVASIADLIRMKRGTGRPQDEEDVRALMKLQ"
BASE COUNT         1211 a         2511 c         2528 g         1001 t
ORIGIN      
        1 tgtagccgac gttcgtcgac agcggcgcga ggtagtccat ccggtcggtg taggggatga
       61 actggttgta ggtcatcccc tcggcgatct tctccatgcc gcggtgcagg tatccgacgt
      121 gcgggacgat cgacttcacc gtctcgccct gcagggtcac cacgaggcgc agcacaccgt
      181 gcgtggaggg gtgctgcggt cccatgttga ggaccatctc ctcccccccg agcgagtcgt
      241 ccgcggcggg gtgctcgacc ggcatcgccg gaggatgcgg cgtcatgtcc ggggccgtcg
      301 gcgatccgcc cggcttcgtt tcgggcccgc tcacgcgtgc tccatgtcgc gccacccggg
      361 ggaggccggg cgtccctcgc cgtcgatcgc cgcggggccc ccctcgacgt cgaagtcctt
      421 gcgcagcggg tggtccgggt agtcctcggc catgatgatg cggcgcaggt cgggatgccc
      481 cgtgaagcgg acgccgacga ggtcgtactg ctcccgctcc ggccagttca tgccggccca
      541 gatcgacgtc aggctggggg ccgtctcccc cggacgaagg cgcagcttca ggcgcaacct
      601 gtgatttttc tccagggaat agacctgcca catcacgtcg tgctcgaact ccccctcgag
      661 gaagtggacc gccacgcagg cgatcggcat gtcgaaagag gtctccgcat cgtcgcgcag
      721 gaaggttgcc gcgtcgacga ggcgctcggc gggcaaggtg acggtgatct ccccccggaa
      781 ctcctccgct ccgatcccgg cctccggaaa gcgggcgcgc aggagatcga cgacgtggcg
      841 cggcgggttc ggctcgatgt agggcccttt catggccccg tcagtcccac tcgagggcgc
      901 cgcggcgcca gcagtagacg tagccgacca ccaggaccag gaggaagacg aacatgcccg
      961 cgaacccgcg catccccatg tcgcggagca cgaccgccca cggatacagg aacgccgctt
     1021 cgatgtcgaa caggatgaac agcatcgcca ccatgtagaa gcgcaccgag accggcttgt
     1081 tcgaggcgtc cagcggatcg acgccgcact cgtaggggga gaggtcggtg cggctcctct
     1141 tcgggcggcc gagcagcgtc gccagggtca ggatgacccc cgcgaggatg agcgccagca
     1201 cggcctgaag cccgatgggg atgtagtcga acacccggcg tcacacaccc ggccggggag
     1261 gcccggcgcc cggcctccct ctcgtgaacc tgctcatccg gcgccgcagg gccttgttca
     1321 agaaggcgga tgctagcacc ccctttccga gggtgtcaac gcgagtagaa tcacccgcac
     1381 cgccatgatt caagaggggt tgcgatgacc gccgtgccgc cgcggacccc ctccgcgccg
     1441 gccgatctcc cccccgaaag cgaggtcgag atcagcgcca tccgggcgca ggggaagggg
     1501 gggcagaacg tcaacaaggt ctcgaacgcg gtccacctgc gcttcgacat ccgcgcctcc
     1561 tccctgcctg aggagatcaa ggagcggctg ctggggatgc gcgaccgccg catcagcgcg
     1621 gagggggtgg tcgtgatcaa ggcacagacg gagcggagcc tcgagcagaa ccgggaggcc
     1681 gccctcgggc gtctgcgggc catggtggcg gcggccgccg cggtgccgcg gacgcgccgg
     1741 ccgacgcgcg ccacgagggg ctcgaaggag cgccgcgtcg agggcaagaa gcggcgcgcg
     1801 cacgtcaagg cgcttcgccg tcgggtcgag gaatgacccg ccccgggacg ccggccgctc
     1861 cgggtgcgct atcctcccgc gtcatgcgac gctccccggg gttccgccgg ctcgtcccga
     1921 tcctgttcct gtccgccgtt cccgtcatcc acgcggcggc ggtcgctccg gcgaagccgg
     1981 gcgccggcga cctcgccgcg gcgcgggcgg tgttcgagaa gaacctcgac gccataaggc
     2041 ggcgggatca ggaggcctac ctctcttgtt acttgaagtc ccccaccttc gtccggaccg
     2101 gggccgacgg gcccatgaag gggtacgact ccctcgcgaa ggagccgccg gagtggccgg
     2161 atgccttcga aggactggcg ctcgacctgg tgccgctccg gcccgggctg gtgtacggga
     2221 cgtaccgcta ccgggtccgc tacggcgggc gcgaggagtc cgggatctcg gagcgcttct
     2281 tcgtgaagac cgatgccggc tggaagatcg cggtcagcac cgccttcgcc gcccctcccg
     2341 gcaccccgcc gccgccgcgg gcgctcaccg gcgccaccct catcgacgcc accagcgccg
     2401 ccccggtcgc ggactcggtc gtcgtcctgc gcggcgggaa gatcgactgc gccggaacgc
     2461 gggcccagtg cccgatcccc gaaggggtgg agcggctcga cctcaagggg atgtgggtga
     2521 ctcccggcct cgtggacgcg cacatgcacc acgcccagac cggctgggcg gacgggcgtc
     2581 ccgatttcat cgacgttcgc gacaagcatc cgtacgagac cctccaggca ggcctgcggg
     2641 cacaccccga gcggtggtgg aggtcgtacc tttgttcggg cgtgacggcg gtcttcgacg
     2701 tcggcggcta tccgtggtcg tgggacctcc ggtcgcgggc cggggctgat ccgctggcgc
     2761 cgcacgtggc ggcggccggc ccgctccttt ccaccctcga tttctggctc aacctcccgg
     2821 cggagcggca gttcatctac ctcggcaccg aaaaggacgc gcgcagcgga gtgacctacc
     2881 tcgccgacca cgggaccgac gccgtcaagg tctggttcat cccggtcgcc tcgcggaact
     2941 tcgacgagat ggcgcaggcg gtgaaggcgg ccggccagga agcccatacc cgcgaggtgc
     3001 cgctcatcgt gcacgccacc ggcctcaaag aggcggtcgc ggcgctgaag gcgggggcgg
     3061 acctcctggt gcacagcgtc gaggacgcgc cggtgaatga cgaattcctg cgcctggcga
     3121 agtcgaacgg caccatctac tgcccgacgc tcaccgtgat cggcggctac cggcggctgc
     3181 aggaagcggc cgcggcgcac caggtcccgg cggtggacga cccgaacggg tgcaccgatg
     3241 cggagacgac gaagaacctc gccgaaaccg ccaccctcac gatgaagccg gacgatcccg
     3301 gtgtcgccga gcgccgccgg cagcgcttcg agaccttcgc gaagatgacc gccgcgaacc
     3361 tcaagacagt gcgcgacgcc ggcatcccga tcgccatggg gaccgacgcc ggcaacccgc
     3421 tgaccctgca cggcgtgtcg gtctatgccg agatggaggc gatgcaggcg gccggcctga
     3481 cggcgatgga ggtcctcgtc gcctccaccc gcaacggcgc ccgcgccatg cgccgcgacg
     3541 cggacttcgg caccgtcgag aaggggaagg tcgcggacct gctcgtgctc ggcgccgatc
     3601 cctcgaagga catccgcgcc atgcggcaga tccgattcgt cgtccgcggc ggagtcgtgc
     3661 gcgctcagag cgagctgcgc ccggagccgc gcccgcccca ggagcactag gcgggagcgc
     3721 cgtaggtgaa tcggcgccag aggcgctcga gcggtcccat cgagtgggac cgcagccacc
     3781 acacgctgaa ggcggcctga ccgagcccga tgagcagcgc cgcgagaacg ccgcgcgtcg
     3841 gggagatgcg atcgaacagg ccgaacgcga gacagatcgg caccgcgatg acggcctgca
     3901 ggacatagtt ggtgaacgcc atgcgtccga cggcgcggag ccaggagaga cgatcgggcg
     3961 tgcccgcccc gaggacgaag aaggccaggg cgagataggc ggatgccacc gcccaggcgg
     4021 tcgcgtgcgc gaggagatcg cggcccaggc actcgaggct cgccggagcg cagtcggacc
     4081 gcccctgcca tacgaccagc gccagcgtca tgaccccggc cacggccgca gccaccgcca
     4141 ggaagcgccg ctgcagcggg cgatcgatcg ccagccgctc gaagaggcgg gcgcggccgg
     4201 cgaggacacc ggcgagcatc agtccgatga cccgcggcca ggagcgcagc gggttggtga
     4261 cgaaccagtg ccgcagccat tccaggttct cgaggacgta ggggcggccc gccgccgaag
     4321 gccgggcggg ccaggcgagg ccgaggcgcg gcacggccca ctcgaagagc gtcggcaaca
     4381 gcaggaagac gacgatgcat ccgaccagga gcgggcccga accacggcgg aagagaagga
     4441 gcgccaggcc gagcagcgcg tacggcagga ggatgtcgcc gttccgcagg aagacggcgt
     4501 gcagaagtcc gaggccgccg aggaatccca tgcgccgcag gtgcaggcgc cggacgtccg
     4561 cccccgccgc ctcccagcgg cgccactgct gcgcgacacc gacgccgaag aggaaggcga
     4621 acagcgtcag gaaccggttg tcgaccagga gccccatgag ccgctcgacc agtcgatcga
     4681 ggcggctcca ctccgcctcc ggcggattcc ccaggctcca cacggtgtag gcgagcagga
     4741 cgccgagaat ggctgcaccg cggatcgcgt cgagggcgtg gacgcgctcc gtggccgagg
     4801 tcggacgcgc cgcgggatcc ggacgagact gcggagatgc gatcgctcgt tcgttcatgc
     4861 tgccggcgag ccggcgccgc gcagcatagc cggactctac cgggtccggg gacggatcgt
     4921 caggaccgga acgcgcgcgt gccgcacgac tttctcggcg atgctgccga tgagcaggcg
     4981 cgccaggccg gtgcggccgg cggtggacat gacgatgaga tcggccccgc gcgaggcttc
     5041 gtcgatggcc tgcagcggat cgtcgatgac cacgcgcacc tccacgcggg gccgggcgcc
     5101 tcgcaccgtg cgcgccacga gtccttcgag gcgtccgcgg gccgcccgca cctcctcccc
     5161 ctccggaaga taagtccgcg cggcgatcat gtgcgcggtg atcggctgga agggagggat
     5221 ggcgtgcagc acgatgagac gtccgccgcc ggccttcgcc agtccggcag catattcgag
     5281 ggcgcgcgtc gcatgacggg agaaatcgtg cggaaccagg atccttcgga aaaggttcat
     5341 ggatcgtctc tagcatgcct ttccgtcggc gaccagcgcg cgttgtattc tggagccgat
     5401 cccggaacgg gaggaggtgc ggcatgaccg tgcgcgtcgg catcaacggc ttcggccgga
     5461 tcgggcgtct gttcctgcgg gcggcgaaga gccgcgacct gccgttcaac ttcgtcgcca
     5521 tcaacgatct cgccgacgtg gaccagatgg cgctcctcct gcgctacgac tcgatccacg
     5581 gcccgtatcc gggcaaggtc gaagccgagg acgatgactt gatcgtggac ggcgaccgca
     5641 tccgcaccca caaggtcacc gacaagcggg aaggggatcc attcccgttc ccctggggag
     5701 aggtcggcgc cgacgtcgtc gtggaggcga ccggggtctt ccgtcgcatc gccgagctgc
     5761 agcggcacat cgccgccgga gcgaagaagg tggtcctgac cgtccccgcg aaagatccgc
     5821 tcgaggcgac cgtcgtcatg ggggtcaacg atcacatcct gaaggcctcg gaccggatcg
     5881 tctcgaactc ctcctgcacc accaactgcg ccgcgccggt cgccaaggtc ctccaggatc
     5941 tctatggcat ccgcgaaggg ttcctgacga ccgtgcacgc ctacaccaac gaccagcgcc
     6001 tcttcgatca cccgcacaag gacgtccggc gcgggcggat ggcgccgatg aacatcgtgc
     6061 ccacctccac cggcgcggcg aaggcgctgg ggctcgtcat cccggcgctc aaggggaaac
     6121 tggaggggct ggcgttccgg gttccggtcc cctcgggatc ggtcgtcgat ctcaccgtca
     6181 tcaccgagaa gcgcccggcg gtcgatgaga tccacgccgc catgaaggcg gccgccgagg
     6241 ggccgatgaa ggggatcctc caggtcacgg aggacccgat cgtctccgcc gatgtcatcg
     6301 gcaatccgca ctcgtcgatc tacgacagcc tgctcacgca ggtgccgcac gaggacatgg
     6361 tcaagatcgt cgcctggtac gacaacgagt ggggctacgc catgcggctc gtcgatctcg
     6421 tcgagaaggt cgcggcgctg ggctgagcga agccccggcg cgcctcatgg cacggatgcg
     6481 ccggctcgac ggatctcagg tctcgatccc gagccagtcc cgcatgaggc gcttgatctg
     6541 agtctggtac cgcacgcccg cgagatcgca gcgccaccgg aacgcgttca ggagatcctg
     6601 cggaaccttg aggctgatga ggcggcttcc ggacgacgcc tcggcatgaa gccgccggaa
     6661 gctctccagg aaatcgagca cctcgtccga cttcatcgcc ttgcaatgct cgaggtactc
     6721 gtcggagaag tgctgcacgg gacggatggg cgtcttcctc actggagttt catcagagcc
     6781 ctgacgtcct cttcgtcctg aggcctgccg gtcccccgct tcatccggat gagatcggcg
     6841 atcgacgcaa ctcgcaggat tccaccccgg accgcgattc tccgggtctt catgtgggcc
     6901 aggtcctcgg tcaggatcac gtcgacgatc tccgacggtc gcgccgggtt gacgaaggtc
     6961 caggcgcgca tgttccggtt gcgaatgtac tcctccctga actggaagac ctcggaggcg
     7021 gcgaccggca gccgcggccg cagcccgagg tcgaggaaca gcgtttccgt cttgcggaaa
     7081 tcggcggccc gcagccggat gacgagatcg atgtcgacag tcccccgcac ggctccgtgc
     7141 agcgccacgg catacccgcc gacgatcgcg tagggcagac gtgcccgatc gaaggcctcg
     7201 gccacccgaa gaatgaacat ggtatctccc agtatatacc tatcgcggcg g
//
LOCUS       DASRIQ010000392         6464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_938064, whole genome shotgun sequence.
ACCESSION   DASRIQ010000392 DASRIQ010000000
VERSION     DASRIQ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938064"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="VFD06_09590"
     CDS             complement(<1..1895)
                     /locus_tag="VFD06_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN03823.1"
                     /translation="MRRIDAKGQRVVDAPLLGVAPRLVEPGLRLVPRGLFRITTYPEG
                     ETRLRVDLTGARAVTSREGARVDLEVILAYALPPARLLDLHRAHGPDVASWIEESVRA
                     AVGARLAQVSYDLLRERDPRLTQQARADVEKRLSDGVAVASLRLVQVAGAGEAGGDIV
                     RAGATPLPRRIVLVGLDSYTWRIADPLMRQGRMPHLKAIVDRGVRANLRTITPILSPV
                     IWTSIATGMKPSKHGIVDFVVSARDTGALIPVTSSMRRVPALWNVLGRQEVACDAVAW
                     WASWPAEAVPGRIVSDRVAFQLFEQGVQDWKSTDPEKARGKTSPADLFPTLLPMIKAP
                     SEIRDAEVAPYLGSGRGPAAADRLPDDLTPEQADRIRQFRTVLAAGQTYHAIARHLLQ
                     HPAAAPGAPSVSLFYYEGPDTTSHLFMRDRPPLMPGVNPQDSALFGAMVDRYYETQDR
                     YLGEIVRDAGKDATILVVADHGFKSGEDRPVDSDPRIDRGRAGEWHTPVGVLAIAGPD
                     VRRGVDIDAASILDITPTILALLGLPTARDMDGQPLTEAFTPEFLRSHPVAWIDTYGG
                     ARPEDDDSLVASADDAELVEKLRSIGYIGDERLTAQNNRGLIALDDGDVEGAIAHFEG
                     ALAKGAG"
     gene            complement(1971..2987)
                     /locus_tag="VFD06_09595"
     CDS             complement(1971..2987)
                     /locus_tag="VFD06_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03824.1"
                     /translation="MTLRRLPLAILILACLLPAAVSEAPAQTNTTGVLYALSPASAFE
                     EGCFDPCLCIVHYNDGLVGTWRLTPAPPEPQFAVYRIDQISWLVPGLDRRVTGSGTYR
                     TGGEFARTHRLELDLTIDDQPPQHFDSGLQLGGAEFPAIALTVSMNNLVCHDTVFHVN
                     AAPIVPKLVPYGLFGSTYDEGCFGPCDCAVVSRPLLGRFGLMRLRSDEEGIDYAVLDV
                     RWMRGSPTAVANDTIPVTGHGIYRTRRGGGITTVMPSHRMTLYLSENGQAPVRFDSGI
                     VTGGEHGPGGGPPKRIDIDLAEHGFACLDRVYSLHARRRTAATADFGAIDADPAPVPV
                     TPVP"
     gene            complement(3098..4132)
                     /locus_tag="VFD06_09600"
     CDS             complement(3098..4132)
                     /locus_tag="VFD06_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03825.1"
                     /translation="MTLHRLPIGVLLGACLLLAAPSMVSAQTNTSDVLYVLNPASGYE
                     DGCWDPCLCPVHLTDGLAGTFRMRPAPPEPGFDVYAIDQVNWFLPQFDQRITGSGTYR
                     IGGEFARMHQLVLDLVQDGRPLQHFDSGLILGGADFPAITISVSMNNYVCHDTAFHVA
                     AAPVVPKLVPYGLFGSEYLEGCFGPCDCLVVSKPLHGRFGLMKLRSSNGGVEGPSVDY
                     AVLDVRWMVRSASTSPANDSYPVTGYGIYRTRPGGGVITVMPSHRMTLFLSENGRPPV
                     RFDSGIVPGGEHGPGGGPPKRIDIDLAADGFACTDRVYSLNARRSNAATMDFGVIDSD
                     PAPIPVTPVP"
     gene            4537..5322
                     /locus_tag="VFD06_09605"
     CDS             4537..5322
                     /locus_tag="VFD06_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HZN03826.1"
                     /translation="MSESAWGEENAGRGAAVADAAEDLLPRGDDRPGVAPRTRPAPAP
                     LYSVSLKLEGRLAVVVGGGPIALRKATELLHAGARVWAIAPEWPADFSGLEGDPDKGS
                     RLLRVTRRFTAGDLVGAFLVVAATDDVSVQRAVARAAESQGVLCNVVDVNDLCSFYVP
                     ATLRRGSLQIAVQTDGRFPLLAVALRDRLARIVGPSFAEALDRLAAARAVVRERFPAA
                     GDLDRRTEALRRLLPEEALDLLLDGRLEEFEARVESWRRDFAS"
     gene            5319..6188
                     /gene="cobA"
                     /locus_tag="VFD06_09610"
     CDS             5319..6188
                     /gene="cobA"
                     /locus_tag="VFD06_09610"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZN03827.1"
                     /translation="MTKPLGIVSLVGAGPGDPDLLTRGAARRLRRAEVVYYDALIPPS
                     VLRLCRKNARLVPVGKRRGYATLRQEAIEARLVRDAQKGLRVVRLKGGDPFVFGRGGE
                     EALALQRAGIVVEVIPGVSSGIAAPARAGIPLTHRGLSSSAAFVTAHDLLDGSAAEAV
                     VSRLSHLARGADTLVVFMAGAHLARLAAVLLAAGLDRATPAATIENGTLPGARVRHGR
                     LDELESLGVGRGTGPLLVVVGRTAALASDLAGPLAAGARLAAETGVGAPAATVEPPET
                     AETAAIPFRRTGR"
     gene            6185..>6464
                     /locus_tag="VFD06_09615"
     CDS             6185..>6464
                     /locus_tag="VFD06_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HZN03828.1"
                     /translation="MSDTNPTSLPSETAAEIANIESEIGRFLAGTLAPERFRSFRLAH
                     GIYGQRQPGVQMIRVKIPTGALDGAQLRLLADLVEEFSNGSAHLTTRQD"
BASE COUNT          933 a         2286 c         2328 g          917 t
ORIGIN      
        1 ctcccggccc ccttggcgag ggccccctcg aagtgcgcga tggcgccctc cacgtccccg
       61 tcgtcgaggg cgatgaggcc gcggttgttc tgcgccgtca ggcgctcatc gccgatgtag
      121 ccgatcgatc gcagcttctc gaccagctcg gcgtcgtcgg ccgaggcaac gagcgagtcg
      181 tcgtcctccg ggcgtgcgcc gccgtaggtg tcgatccagg ccaccgggtg ggagcgcagg
      241 aactccgggg tgaacgcctc ggtgagcggc tgcccgtcca tgtcgcgcgc ggtcggcagc
      301 ccgagaagcg ccaggatggt cggggtgatg tcgaggatcg aagcggcgtc gatgtcgacg
      361 ccgcgccgca cgtccggccc ggcgatcgcc agcacgccga ccggcgtgtg ccattcgccg
      421 gcccggccac ggtcgatgcg cggatcgctg tcgaccgggc ggtcctctcc cgacttgaag
      481 ccgtggtccg cgacgacgag gatggtggcg tccttcccgg cgtcgcgcac gatctccccg
      541 aggtaccggt cctgcgtctc gtagtagcga tcgaccattg ctccgaacag cgcggaatcc
      601 tgagggttca ctcccggcat gaggggcgga cgatcgcgca tgaacaggtg cgaggtggtg
      661 tccggtcctt catagtagaa aaggctcacc gacggggccc cgggcgcggc ggccggatgc
      721 tgcagcagat ggcgcgcgat ggcgtggtag gtctgtcctg cggccagtac ggtgcggaac
      781 tgccggatgc gatccgcctg ctccggggtg aggtcgtccg gcagccggtc cgcggcggcc
      841 gggccgcggc cggatccgag gtagggggcc acctccgcgt cgcggatctc cgacggcgcc
      901 ttgatcatcg gcagcagggt cgggaagaga tcggccgggg acgtcttgcc gcgcgccttc
      961 tccggatcgg tgctcttcca atcctgcacc ccctgctcga acagctggaa cgcgacgcga
     1021 tcggagacga tccggccggg caccgcctcc gccggccagg aggcccacca ggccacggcg
     1081 tcgcacgcca cctcctggcg gccgaggacg ttccacaggg ccggcacgcg ccgcatcgag
     1141 ctcgtgaccg ggatcagcgc gccggtgtcg cgcgccgaga cgacgaagtc gacgatgccg
     1201 tgcttggacg gtttcatgcc ggtggcgatg ctcgtccaga tcaccggcga caggatcggc
     1261 gtgatggttc tcaggttcgc gcgcacgccg cgatcgacga tcgccttgag gtgcggcatc
     1321 cgcccctggc gcatcagcgg atcggcgatc ctccaggtgt aggagtcgag cccgacgagg
     1381 acgatgcgcc gcggcagcgg cgtcgccccg gcccgcacga tgtccccgcc cgcctccccc
     1441 gcgcccgcca cctgcacgag ccgcagggac gcaaccgcga cgccgtccga gagcctcttc
     1501 tcgacgtcgg cgcgcgcctg ctgcgtcagg cgcggatccc gttcgcgcag gagatcgtag
     1561 gagacctgcg ccaggcgcgc cccgaccgcc gcgcgcaccg actcctcgat ccaggaggcg
     1621 acgtccggac cgtgcgcccg gtgcagatcg aggaggcgcg cggggggcag cgcatacgcg
     1681 aggatcacct cgaggtcgac gcgcgccccc tcccggctgg tgaccgcgcg cgccccggtc
     1741 aaatcgaccc gaagccgcgt ctcaccttcg gggtacgtcg tgatccggaa cagcccgcgc
     1801 ggcacgagcc ggagccccgg ctcgacgagg cgcggggcga ctccgaggag cggcgcgtcg
     1861 acgacacgct ggcccttggc gtcgatgcgg cgcaccgacg ctcccaggta cagggtcagc
     1921 gcggcgcccg cgaggagcgc gagggcccgg gatcgggaca gagacgattc ctacggaacc
     1981 ggcgtcacgg gaaccggggc cgggtcggcg tcgatcgccc cgaagtcggc agtcgcggcg
     2041 gtacggcgcc gggcgtgcag ggaatagacg cggtccaggc acgcgaagcc gtgctccgcg
     2101 aggtcgatgt cgatccgctt cggcggcccg ccacccggcc cgtgctcgcc gcctgtgacg
     2161 atgccgctgt cgaagcgcac cggcgcctgc ccgttctcgc tcaggtaaag ggtcatccgg
     2221 tggctcggca tcaccgtggt gatccccccg ccgcggcggg tgcggtagat cccgtgtccg
     2281 gtcaccggga tggtgtcgtt ggcgaccgcc gtgggggagc cgcgcatcca gcgcacatcg
     2341 aggacggcgt agtcgatccc ctcctcatcg gagcgcagcc gcatgagacc gaatcgtccg
     2401 aggagcggcc tcgacaccac ggcgcagtcg cacggcccga agcagccctc gtcgtaggtc
     2461 gatccgaaca ggccgtaggg gacgagcttc gggacgatcg gcgccgcgtt gacgtggaag
     2521 acggtgtcgt ggcacacgag gttgttcatc gacacggtca gggcgatcgc ggggaactcc
     2581 gcgcccccca gctgcaggcc gctgtcgaag tgctgcggcg gctggtcgtc gatggtcagg
     2641 tcgagctcga gccggtgggt gcgggcgaac tcgccgccgg tgcgataggt gccggaaccg
     2701 gtgacgcgcc ggtccaggcc cggcaccagc cagctgatct ggtcgatgcg atagaccgcg
     2761 aactgcggct cggggggggc cggggtcagc cgccaggtcc ccacgagccc gtcgttgtaa
     2821 tgcacgatgc agaggcacgg gtcgaagcag ccctcctcga aggcgctcgc gggcgacagc
     2881 gcgtacagca ccccggtcgt attggtctgg gccggcgcct cggatacggc ggcgggcagc
     2941 aggcaggcga ggatcaggat cgcgagcggc aggcgacgaa gagtcatcgt ggacctcctc
     3001 atatcaataa ttaagaagga gcggtgccag catcctgcca caggacggcc gcggcctcaa
     3061 ccggcgcgcc cgtacggagt cgcggcagcg aaagttccta cgggaccggc gtcaccggga
     3121 tcggggcggg atcgctgtcg atcacgccga agtccatggt ggcggcgttg ctccgccgcg
     3181 cgttgagcga gtaaacccga tccgtgcacg cgaagccgtc ggccgcgaga tcgatgtcga
     3241 tccgcttggg cgggccgccg ccgggcccgt gctctccccc ggggacgatg ccgctgtcga
     3301 agcggacggg cggccggccg ttctcgctca ggaacagcgt catccgatgg cttggcatca
     3361 ccgtgatgac ccctcctccg gggcgcgtgc gatagatgcc gtagccggtg accgggtacg
     3421 agtcgttcgc gggcgaagtg ctggcggagc ggaccatcca gcgcacgtcc agcacggcgt
     3481 agtccacgct cggaccctcc acccccccgt tgctggagcg cagcttcatc aagccgaatc
     3541 gtccgtgcag cggcttggag acgaccaggc agtcgcacgg cccgaagcag ccctcgagat
     3601 actccgatcc gaagagtccg tacggcacca gcttcgggac gaccggcgcc gccgcgacgt
     3661 ggaacgccgt gtcgtggcag acgtagttgt tcatcgaaac gctgatcgtg atcgccggga
     3721 agtcggcgcc gccgaggatg agcccgctgt cgaagtgctg cagcggccgg ccgtcctgga
     3781 cgagatccag cacgagctgg tgcatgcgcg cgaactcgcc gccgatccgg taggtccccg
     3841 aaccggtgat ccgctgatcg aactgcggca ggaaccagtt gacctggtcg atcgcgtaca
     3901 cgtcgaatcc cggctccggc ggagcggggc gcatgcggaa cgtccccgcg agcccgtcgg
     3961 tcaggtggac cggacacagg cacggatccc agcacccgtc ctcgtaaccg ctcgcagggt
     4021 tgagcacata caggacatcc gaagtgttgg tctgcgccga gaccatcgac ggcgcggcga
     4081 gaaggagaca ggccccgagc aggaccccga tcggcaggcg atgaagagtc atcgaagacc
     4141 tcctctccag atagagataa aggacagttg cggtttccac atcctgccac agaacgccct
     4201 gtcgctcaag gagatcgctc cgccaccgcg gcctcgccca cccacccccg gcccctggac
     4261 gggggtgggg atcccccgac acacggggcc cttgggggac aagggatctg tccggtccgg
     4321 tcagattctt gttgacaagc ggtgacttga tggtaaattg ccccggtcat gactcataag
     4381 gtgtgctgct gctgttgtac ggacggggtc gagcccccgt ccgcgcccgt ttggaggatg
     4441 aacctctaac cagcacgtag cgcagacgga cttctcggcc cagaaccctc caggttctgg
     4501 gcctttttat ttgcgcggac agcgcggagt gcagagatga gcgagagcgc ctggggagaa
     4561 gagaacgccg gccgcggcgc ggccgtcgcc gacgctgcgg aggacctcct gccgcgcggt
     4621 gacgatcgcc ccggggtggc gccgcggacg cgccccgcgc ccgcgccgct gtattcggtt
     4681 tcgttgaagc tcgaggggcg gctggccgtc gtcgtcggcg gcgggccgat cgcgctgcgc
     4741 aaggccaccg agctccttca cgccggcgcg cgcgtctggg cgatcgcgcc cgagtggccg
     4801 gccgatttct ccgggctcga aggggatccg gacaagggct cccggctcct gagggtgacg
     4861 cgccggttca ccgccggcga cctggtcggc gccttcctgg tcgtggccgc cacggacgac
     4921 gtctccgtgc agcgcgccgt ggcgcgcgcc gccgagtcgc agggggtgct gtgcaacgtc
     4981 gtcgacgtca acgacctgtg ctccttctac gtgccggcga cgctgcggcg cgggtcgctt
     5041 cagatcgcgg tgcagaccga cggccgcttc ccgctcctgg cggtcgccct gcgcgaccgg
     5101 ctggcgcgga tcgtcggccc gtcgttcgct gaggcgctcg atcgtctcgc cgcggcccgg
     5161 gccgtcgtgc gggagcgctt cccggccgcg ggcgacctcg atcggcggac ggaggcgctg
     5221 cgccgcctgc tgcccgagga ggcgctcgac ctcctcctcg acggccggct ggaggagttc
     5281 gaggcgcgcg tcgagtcctg gcgcagggac ttcgcgtcat gacgaagccg cttgggatcg
     5341 tgagcctggt gggcgccggc ccgggggacc ccgatctgct gacccgcggc gccgcgcggc
     5401 ggctccgccg cgccgaggtc gtctactacg acgccctcat cccccccagc gtgctgcgcc
     5461 tgtgccggaa gaacgcccgg ctggtcccgg tcggcaagcg gcgcggctac gcgacgctcc
     5521 gccaggaggc gatcgaggca cggctcgtcc gcgacgcgca aaaagggctc cgggtcgtcc
     5581 ggctgaaagg aggcgacccg ttcgtcttcg gccgcggcgg cgaggaggcg ctggcgctgc
     5641 agcgggccgg gatcgtggtc gaggtgatcc cgggggtctc ctccggcatc gcggcgccgg
     5701 cgcgggccgg catcccgctc acgcaccgcg gcctgtcctc gtccgccgcc ttcgtcaccg
     5761 cgcacgatct tctcgacggc tccgcagcgg aggccgtcgt ctcgcggctg tcgcacctgg
     5821 cgcgcggcgc cgacacgctg gtcgtcttca tggccggggc gcacctcgcc cggctggcgg
     5881 ctgtcctcct cgccgccggc ctggaccggg ccaccccggc cgccacgatc gaaaacggca
     5941 cgctccccgg cgcccgcgtc cggcacggga ggctcgacga gctggaatcg ctcggggtcg
     6001 ggcgcggcac cggccccctc cttgtggtcg tcggccggac cgcggcgctc gcctcggacc
     6061 tcgccggtcc gctcgccgcc ggggcgcgcc tcgccgcgga gaccggcgtg ggtgcgcccg
     6121 ccgccaccgt cgaacctccg gagaccgcgg agaccgccgc cattcccttc aggaggacgg
     6181 gacgatgagc gacacgaacc cgaccagcct cccttccgag accgccgccg agatcgccaa
     6241 catcgagtcg gagatcggcc gcttcctcgc cggaacgctc gcccccgaac gcttccgctc
     6301 cttccgcctg gcgcacggca tctacggaca gcgccagccc ggcgtgcaga tgatccgggt
     6361 gaagatcccg accggagcgc tcgacggtgc gcagctgcgt ctgctcgccg atctcgtcga
     6421 ggagttcagc aacggctcgg cgcacctgac gacgcggcag gacg
//
LOCUS       DASRIQ010000393         8273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1216635, whole genome shotgun sequence.
ACCESSION   DASRIQ010000393 DASRIQ010000000
VERSION     DASRIQ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216635"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..463
                     /locus_tag="VFD06_09620"
     CDS             86..463
                     /locus_tag="VFD06_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZN03829.1"
                     /translation="MRGSASEGSRDRLTVHALKVMAHIGVTEEERRERQRLEVDVELF
                     ADLESAGRSGDLSCTIDYRQVADGLRGLLEGRPWHLVEAAARASLDLLLERFPSADRA
                     VVRVRKYVLPDTAHVEIAMERRR"
     gene            518..1105
                     /locus_tag="VFD06_09625"
     CDS             518..1105
                     /locus_tag="VFD06_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HZN03830.1"
                     /translation="MILETLVTTIDRDGGAHCAPMGIGLEEGHLVLRPFRTAATCANL
                     EATGVGVVNFTDDVFLFARCALTPCLPLVRKASAVAAPILQAACSWREFRVTASDLGA
                     ERALFRAEVVASGRGREFLGFNRAQHAVIEATILATRLHLIDGQVIRAEIDRLRPLVE
                     KTAGAREREAFAYVRRFVEEKDAGKDAVPEGGERR"
     gene            1102..2175
                     /locus_tag="VFD06_09630"
     CDS             1102..2175
                     /locus_tag="VFD06_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase family protein"
                     /protein_id="HZN03831.1"
                     /translation="MSIRVTAHARLHFGFLDLSEDRAGRRFGGLGMALSTPRLMIRLE
                     RGRGLDVEGPLAERIESMALRVHRALELRPQARIVVEEAIPEHVGLGSGTQMALALAT
                     AIARQRDIDQPVARLCALAGRAQRSGVGFHLFQAGGFVVEGGHARGAVPFEVPPLLAR
                     HDVPEGWRIVVAIPKTTQMVSGDMEEEAFRRLRPAAAVTVDRISRAVLTRLLPAVVSG
                     DLPAFGAALTDVQDLVGGCFAAVQDGAYHPAAEPLVRLLKSKRAIGVGQSSWGPAVYA
                     FAGDDKEEKRLLGILSAAAPGAILLKARPQNQGALRPGPRSVAARAENGRGAPPRAGS
                     GARRPTAARRTGSQPRRRRSLPD"
     gene            complement(2189..3070)
                     /locus_tag="VFD06_09635"
                     /pseudo
     CDS             complement(2189..3070)
                     /locus_tag="VFD06_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
     gene            complement(2971..3873)
                     /locus_tag="VFD06_09640"
     CDS             complement(2971..3873)
                     /locus_tag="VFD06_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HZN03832.1"
                     /translation="MPKIGILAAAEGWHLEDLRRAFAALRFESERIDPTRLVGRTRAM
                     LPDRGAAPAGPPWDDLDALLVRVVPPGSLDQIVFRIDLLHWLADRGLPVVNSPRALER
                     SIDKHWTTRLLEAAGVPTPRTVVTERCDAALAAFRELGDVVVKPLLGSGGRGIFRISD
                     EDHAWRAFRALEQQRFVLYVQEFVPHGRHDLRLFVAGGEAIAAARREGGGWKTNLAAG
                     AKARPHRATPEQERLATAAAAAIGADYAGVDLLEAEDGRLLVTEVNGIPAWEGIGEAS
                     GIDIAAVIASRVAARARARASGGA"
     gene            complement(3866..4834)
                     /gene="mch"
                     /locus_tag="VFD06_09645"
     CDS             complement(3866..4834)
                     /gene="mch"
                     /locus_tag="VFD06_09645"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR03120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HZN03833.1"
                     /translation="MTQHEALSRINPEALLIADRMEAGSAALGVTPHRLANGCRLLDA
                     GVEARGSLQAARHYAEACLGGLGRVRVAPRPLHEAVLFEARVEVDAPLVACLASQYAG
                     WKVQTGSFFAMGSGPARAVRGAEPLFDRFPLRISAEKTVLLLETDRLPDAAVAAAVAE
                     GCGLDPSNLTLIAARTGSLVGSTQVAARSVETALHKLLELGFDLTTIVGGVGTCPIAP
                     GHPDTLRAIGRTNDAVLYGAEVALWVQAEDERIEAILDRVPSCASREHGRLFHDLLRE
                     HGDFYGIDPMLFSPARVTIVNAATGKVHAAGRLDEATLRRSFGLDA"
     gene            complement(4831..5658)
                     /locus_tag="VFD06_09650"
     CDS             complement(4831..5658)
                     /locus_tag="VFD06_09650"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HZN03834.1"
                     /translation="MSLRLTLRRWPHPPGVEAEAPDLLPERLGRLSRSAIEALRVRLG
                     REEQRVGDLFEVDGTPGEDVTLAGDLSAFVRLGAGMTRGRLVVKGNAGLAAGSRMSGG
                     RLEISGDAGARAGEGMTGGVLVVRGSTGDDLGGPPPGAARGVNRGAILVAGNAGARAG
                     RRMRRGLIAIGGGAGPLAGTEMLAGTLVIAGHAGDGIGTWMRRGTILLGTGSPPPASA
                     FADAGSGRFSFWALYDEALRFAGATIPEAWREGRFRRYVGDRSGGGLGEILVPEGGR"
     gene            complement(5655..6614)
                     /gene="fhcD"
                     /locus_tag="VFD06_09655"
     CDS             complement(5655..6614)
                     /gene="fhcD"
                     /locus_tag="VFD06_09655"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HZN03835.1"
                     /translation="MLIEGTFIEDTYAEAFSLRAARLIVTADTPAWAQTAALSMTGFA
                     TSIIACGCEAGMEGPEPETTPDGRPGVRVILLARSAADLQDQVVRRVGQCVMTAAGAS
                     CFDALGTTDESIRLGSLLRFFGDGQQTSKRIDPALAAAAAGRVRPRRLWRVPVMDGEF
                     VVDDRCGIRKAVAGGNFLILGRTAEETLAAAERAVAAIRRLPEVVLPFPGGIVRSGSK
                     VGSKYRKLIASTNSAYCPTLRGVVETALPEEVDSVLEIVIDGLSVEAVGAAMAAGARA
                     ACGPGIVRLGAGNYGGRLGKHLFHLRPLLGGPPDPSTPPSGSP"
     gene            complement(6608..8167)
                     /locus_tag="VFD06_09660"
     CDS             complement(6608..8167)
                     /locus_tag="VFD06_09660"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HZN03836.1"
                     /translation="MTRKEDAPVGTIRIRNGSVWDPRHGRRGDVSDVCIENGRVIASL
                     PATAPVFDATGLVVVPGGVDLHSHIAGGAVRAARLLCPEAAGSLCLPTIDEVGRRYAA
                     MGYTTVFDAAIAPAFAAAAHDELEALPILDSGFFLLMGNDPVTLAACRPDEDAGTDLE
                     AAIAGLLEAAGAYAPKLVNPGGVEAWKRGAEEPSIEAAGDLLCRFARAATALRLPHPL
                     HVHLPRLGLPGNAATTMTLLERFGGARAHVTHAQFHAYGGRTAAGFRSRAPEIASALN
                     RLPHLTADVGQVVFGPVVTLSADGPAQERLHRANHRKWLHLDVEAESACGVVPHDFRA
                     SNLVSAVQWAAGLELMLLAKDPWRIALTTDHPNGGPFAAYPSILRMLADRAYREEILR
                     RAHPGLPGRSHLAGIGREYSFEELLIVTRSAPARILGLERKGHLGPGADGDVALYRLA
                     PDIETTFSQAVATFKDGRLAAREGELVDARPGRRLVAPQVPPLPEAARQRFLERAEAL
                     RASTGAAEATC"
BASE COUNT         1115 a         3023 c         3082 g         1053 t
ORIGIN      
        1 atcgcccggc gcctggggaa gaagtacatc cagggggagc ccctcgaatg gggctacctc
       61 tcgtggaccg aggaggagga agcgcatgcg cggcagcgcg tctgagggat cgcgcgatcg
      121 cctgaccgtg cacgccctca aggtgatggc gcacatcggc gtcaccgagg aggagcgccg
      181 cgagcgacag cgtctcgagg tggacgtgga gctgttcgcc gatctcgagt cggccggtcg
      241 gagcggggac ttgagttgta cgatcgacta ccggcaggtg gcggacggac tgcgcggact
      301 cctcgaagga cgtccgtggc acctggtcga ggcagcggcg cgcgcctcgc tggatcttct
      361 gctcgagcgg ttcccctccg ccgaccgggc ggtggtccgg gtgcgcaagt acgtgctgcc
      421 cgacacggcc cacgtggaga tcgcgatgga gcggcgacgg tgattcaaaa cgagccgaaa
      481 ggcgtgcgat gatcccaggc gagccgaaag gcgagcaatg atcctggaga cgctcgtgac
      541 gaccatcgat cgcgacggcg gggcccactg tgccccgatg gggatcggcc tcgaagaggg
      601 gcacctcgtg ctgcgcccgt tccggaccgc cgccacctgc gccaacctcg aggcgaccgg
      661 ggttggagtg gtgaatttca ccgacgacgt gttccttttc gcccgctgtg ccctgacccc
      721 gtgccttcct ctggtccgga aggcgtctgc ggtcgccgcg ccgatcctcc aggccgcctg
      781 ctcctggcgc gagttccggg tcaccgcgag cgatctcggc gccgagcggg cgctgttccg
      841 cgcggaggtg gtcgcgagcg gccgggggcg ggagttcctc ggcttcaacc gggcgcagca
      901 cgcggtgatc gaagcgacca tcctggcgac gcggctgcac ctgatcgacg gccaggtcat
      961 ccgggccgag atcgatcgtc tgcggccgct cgtggagaag accgcgggtg cgcgcgaacg
     1021 ggaggcgttc gcctacgtcc ggcgcttcgt cgaagagaag gacgccggga aggacgccgt
     1081 cccggaggga ggggagcgcc gatgagcatc cgggtcacgg cacatgcgcg gcttcacttc
     1141 ggcttcctcg atctctccga ggatcgtgcc ggccgccgct tcggcggttt gggcatggcc
     1201 ctctcgacgc cgcgcctgat gatccgtctg gagcgcggcc gcgggctcga cgtcgagggg
     1261 ccgctggcgg agcggatcga gtcgatggcg ctccgcgtcc accgggcgct cgagctcagg
     1321 ccgcaggcgc gcatcgtggt cgaggaggcg atccccgagc acgtgggcct cggctcgggg
     1381 acgcagatgg ccctggccct cgccaccgcc atcgcgcggc agcgcgacat cgaccagccg
     1441 gtggcccggc tctgcgcgct ggccggacgg gcgcagcgat cgggggtcgg cttccacctg
     1501 ttccaggcgg gcggtttcgt cgtcgagggg gggcacgccc gcggggcggt gccgttcgag
     1561 gtaccgccgc ttctggcccg gcacgacgtt ccggagggct ggcgtatcgt cgtcgccatt
     1621 ccgaagacga cgcagatggt gagcggcgac atggaggaag aggcgttccg gcgcctgcgc
     1681 cccgcggcgg cggtgacggt cgaccgcatc tcccgcgccg tcctgacgcg cctcctgccg
     1741 gccgtcgtca gcggcgatct gccggcgttc ggcgcggcgc tgaccgacgt tcaggacctc
     1801 gtcggcggct gcttcgcggc cgtgcaggac ggggcgtacc atccggctgc cgagccgttg
     1861 gtgcgcctcc tcaagtcgaa gcgggcgatc ggcgtcggcc agtcctcgtg gggtccggcc
     1921 gtctatgcct tcgccgggga cgacaaagaa gagaagcggc tcctggggat cctcagcgcc
     1981 gccgcccccg gggcgatcct cctgaaggcg cggccgcaga atcagggggc gctccgcccc
     2041 ggcccgcgga gcgtcgcggc gcgagcggag aatggccggg gcgctccacc gcgcgccggc
     2101 tccggcgcgc ggcgtccgac agcagccaga cgaacaggct cgcagccacg acgtcggcgc
     2161 tcgctcccgg attgatcggc ggccgcgccg cgcgcagcct ccggtcgagc cgctcgagaa
     2221 ggcgccgccc cgcgggccgc agcgggcccc ccgcctgcag gacgatccgg gcgtcgcgct
     2281 gcagccgccg cgccgcggac gcaccgtgcc ggcgcgcgac cagggtgtcc gggtccgcgg
     2341 cgagcagcgc caggtgggag aggacgatcg cctgctccag gctggcgccg cgccgccgcg
     2401 ccgcgtcgat cgccggcagg ccgatgttga aggtggactc gaacgccgtc gcgtactcgc
     2461 gcgccacggc gtcgcgtccg gccgcgagcc gcatcgcatc gaggaggctc acggtcggtg
     2521 cgcggcggac gtcttctctc ccgacccgtc cgagcccgcc gggccgcgcc aggcggatcg
     2581 ccgcgtaggc ttcacgggcg tcgccgacat cgaggtcgcc caggatccgg ccgagccgcg
     2641 acctcagatc ccccgggccc ggaaggagcg ccgccttcgc cagcggcgcc aggagcagca
     2701 cgatgccgag gtgggtgttg gtgcggactt cggcgcggct cctggcgatc gtggcgcgca
     2761 cgagcgcccc gacgcgcgcc cggagggcca ccctgcggaa cacatccccg aggagcgcgg
     2821 cgctcacgac gaagtcgcga taggtcagac cgggccgttc gcggccgggg ctgacgttcc
     2881 ccggcttcgg cgcgtgcgcc tcgaggaggc acgcggtctc ggcggccgcc gcgacgagcg
     2941 gaggggtgag gcggcgggcg cggctccggt tcacgcgccg cccgacgccc gggcccgggc
     3001 gcgcgccgcc acccgcgaag cgatgacggc ggcgatgtcg atcccgctcg cctcgccgat
     3061 cccttcccac gccgggatgc cgttgacctc ggtgaccagg agccgcccgt cctcggcttc
     3121 gagcaggtcg acgccggcgt agtcggcgcc gatcgcggcc gccgcggcgg tcgccaggcg
     3181 ctcctgctcc ggcgtggcgc ggtgcggccg cgcctttgcc ccggccgcga ggttcgtctt
     3241 ccagccgccc ccttcccgcc gcgccgcggc gatcgcctcc ccgcccgcga cgaacaggcg
     3301 caggtcgtgc cgcccgtgcg ggacgaactc ctggacgtag aggacgaagc gctgctgctc
     3361 gagggcgcgg aacgcgcgcc aggcgtgatc ctcgtcggag atccggaaga tcccacgccc
     3421 gccggatccg aggagcggct tgaccacgac gtcgccgagc tctcggaacg ccgcgagcgc
     3481 cgcgtcgcag cgctcggtga cgacggtgcg gggcgtcggc accccggccg cttcgagcag
     3541 gcgcgtcgtc cagtgcttgt cgatgctccg ctcgagcgcc cgcggcgagt tcaccaccgg
     3601 caggccgcgg tcggcgagcc agtgcagcag atcgatccgg aagacgatct gatcgagcga
     3661 gccgggaggg acgacgcgca ccaggagcgc gtcgaggtcg tcccagggag ggccggccgg
     3721 cgccgccccc cggtccggga gcattgcgcg agtgcgcccg acgagccgcg tcggatcgat
     3781 ccgctcgctt tcgaatcgca aggcggcgaa ggcgcgcctc aggtcctcga ggtgccaccc
     3841 ttccgccgcg gcgaggatcc cgatcttagg catcgaggcc gaaggaacgc ctgagcgtcg
     3901 cctcatcgag gcgcccggcc gcgtgcacct tgccggtggc ggcgttgacg atcgtgaccc
     3961 gggccgggct gaagagcatc gggtcgatcc cgtagaaatc gccgtgctcg cgcaggaggt
     4021 cgtggaacaa ccggccgtgc tcgcgcgacg cgcaggacgg cacccggtcg aggatcgcct
     4081 cgatgcgctc gtcctccgcc tgcacccaca gcgccacctc cgccccgtac agcaccgcgt
     4141 cgttggtgcg gccgatggcg cgcagtgtgt cgggatgccc cggggcgatc ggacaggttc
     4201 cgacgccccc cacgatggtc gtcaggtcga agccgagctc gagcagcttg tggagcgccg
     4261 tctcgaccga gcgggccgcc acctgggtcg atccaaccag gctgccggtg cgggcggcga
     4321 tgagggtcag gttcgacgga tcgaggccgc acccttcggc cacggcggcc gccacagcgg
     4381 cgtccggcag gcggtcggtc tccagaagga ggaccgtctt ttcggcggag atgcgcagcg
     4441 ggaagcgatc gaagagcggc tcggcccccc gcacggcgcg cgccggtccg gagcccatgg
     4501 cgaaaaacga tccggtctgc accttccatc cggcgtactg cgacgcgagg caggcgacga
     4561 gcggcgcgtc cacctcgacg cgcgcctcga acagcaccgc ctcgtgaagc ggccgcggcg
     4621 ccacgcgcac ccgtccgagg ccgccgagac aggcctcggc gtagtgccgc gccgcctgaa
     4681 gcgatccgcg cgcctcgacc cccgcgtcga ggagacggca gccgttcgcg aggcggtgcg
     4741 gggtcacgcc gagcgccgcc gatcccgcct ccatccgatc ggcgatgagg agcgcctccg
     4801 ggttgatgcg ggacagcgcc tcgtgctgcg tcatcggcct ccctccggga cgaggatctc
     4861 tccgagcccg ccccccgagc gatctccgac gtagcgccgg aaccggccct cccgccaggc
     4921 ttccggaatc gtcgcgccgg cgaagcggag cgcctcatcg tacagggccc agaaggaaaa
     4981 gcgtccgctg cccgcgtcgg cgaaggcgga cgcgggcggc ggcgatcccg ttcccaggag
     5041 gatcgtcccg cggcgcatcc aggttccgat gccgtccccc gcgtggccgg cgatgacgag
     5101 ggtgccggcc agcatctccg tgccggcgag cggcccggcg ccgccgccga tggcgatgag
     5161 accgcggcgc atccgtcgcc cggcccgcgc cccggcgttc cccgcgacga ggatcgctcc
     5221 gcggttcacg ccgcgggccg cccccggagg aggtccgccg aggtcgtcgc cggtcgatcc
     5281 gcgcacgacg aggacgcccc cggtcatccc ttcgccggcc cgcgccccgg cgtcgccgga
     5341 gatctcgagt cgcccgccgc tcatccgcga gcccgccgcc agccccgcgt tccctttgac
     5401 caccaggcgg ccccgggtca tgcccgcgcc gagccgcacg aacgccgaca gatcgcccgc
     5461 gagcgtgacg tcctctcccg gcgtcccgtc gacttcgaag agatcgccga cccgctgctc
     5521 ctcccggccg agccggacgc gcagcgcctc gatcgcgctc cgcgagagac ggccgaggcg
     5581 ctccggcagg aggtcgggag cctccgcctc cactcccggc ggatgcggcc accgtctcag
     5641 cgtcagcctg aggctcatgg cgatcctgaa ggcggcgtcg aggggtccgg cggacccccg
     5701 aggagcgggc gcaggtggaa caggtgcttg ccgaggcgtc cgccgtagtt cccggcgccg
     5761 aggcggacga tgccgggtcc acacgccgcg cgggcgccgg cggccatcgc ggcgccgacc
     5821 gcttcgacgc tcagcccgtc gatgacgatc tccagaaccg agtcgacctc ctccggaagc
     5881 gccgtctcga ccacgccccg aagcgtggga cagtaggccg agttggtcga ggcgatgagc
     5941 ttgcggtact tcgagcccac cttgctgccg ctgcgcacga tgccgccggg aaaggggagg
     6001 acgacctcgg ggagccgccg gatggcggcg accgcccgct ccgccgcggc gagggtctcc
     6061 tccgcggtgc ggccgaggat gaggaagttc cctccggcga ccgccttgcg gatgccgcac
     6121 cggtcgtcca ccacgaactc gccgtccatg accgggacgc gccacaggcg ccggggccgc
     6181 acccgcccgg ccgccgccgc ggcgagggcc ggatcgatcc gcttgctcgt ctgctgaccg
     6241 tctccgaaga aacgcaggag cgaaccgagc cggatcgact cgtccgtcgt ccccagggcg
     6301 tcgaagcagg aggccccggc ggccgtcatg acgcactgcc cgacccgccg caccacctgg
     6361 tcctggagat ccgccgccga tcgggcgagc aggatcaccc gcacgccggg gcggccgtcc
     6421 ggagtcgtct ccggctcggg cccttccatc ccggcttcgc agccgcaggc gatgatggaa
     6481 gtcgcgaacc cggtcatcga cagcgccgcc gtctgtgccc aggcgggggt gtcggcggtg
     6541 acgatgagtc gcgccgcacg cagggagaac gcctcggcgt aggtgtcttc gatgaaggtc
     6601 ccttcgatca gcacgtggcc tccgcggcgc cggtcgaggc gcggagcgct tcggcccgct
     6661 cgaggaacct ctgccgggcg gcctcgggga ggggcgggac ctgcggagcc acgagccgcc
     6721 gccccggccg cgcgtcgacc agttccccct cgcgcgccgc gagccggccg tccttgaagg
     6781 tggcaacggc ctgagagaag gtcgtctcga tgtccggggc gaggcggtaa agcgccacgt
     6841 cgccgtccgc acccggtccg aggtgccctt tccgctcgag cccgaggatg cgggcgggcg
     6901 cggagcgggt gacgatcagg agctcctcga aggagtattc gcggccgatg ccggccagat
     6961 ggctccggcc cggcaggccg ggatgcgcgc ggcgcaggat ctcctcgcgg tacgcccggt
     7021 ccgccagcat ccggaggatc gaagggtacg cggcgaacgg tccgccgttc gggtggtcgg
     7081 tcgtcagggc gatccgccac gggtccttcg cgaggagcat cagctccagc cccgccgccc
     7141 actgcaccgc cgacaccagg ttcgaggcgc ggaagtcgtg cggcacgacg ccgcaggcgc
     7201 tctccgcttc gacgtcgagg tggagccact tgcggtgatt ggcgcggtgc agacgctcct
     7261 gcgccggtcc gtcggcactg agcgtgacca ccgggccgaa gaccacctgt ccgacgtcgg
     7321 ccgtcaggtg cggaaggcgg ttcagggccg acgcgatttc cggcgcccgg ctgcggaagc
     7381 cggcggcggt ccgcccgccg taggcgtgga actgggcgtg ggtgacgtgc gcccgcgcac
     7441 cgccgaaccg ttccaggagc gtcatcgtcg tcgccgcgtt gccgggaagc ccgagccgcg
     7501 gaaggtgcac gtgcagtggg tgcggcagcc gcagcgccgt cgcggcccgg gcgaaccggc
     7561 agaggaggtc gccggccgcc tcgatcgagg gctcctcggc gccgcgcttc cacgcctcga
     7621 ctcctccggg gttgaccagc ttcggcgcgt aggcgccggc ggcttcgagg agcccggcga
     7681 tcgccgcctc gaggtcggtc ccggcatcct cgtcgggacg gcaggcggcg agggtgaccg
     7741 ggtcgttccc catgagcagg aagaaccccg agtcgaggat cggaagcgcc tcgagctcgt
     7801 cgtgcgccgc cgcggcgaac gccggcgcga tggcggcgtc gaacaccgtg gtgtagccca
     7861 tggccgcgta gcgccggccg acctcgtcga tcgtcgggag gcagagggaa ccggccgcct
     7921 cgggacagag gagacgcgcg gcgcgcacgg cgccgccggc gatgtggctg tggaggtcga
     7981 cgccgcccgg gaccaccacg agccccgtcg cgtcgaagac cggcgccgtc gccggcaggg
     8041 atgcgatcac gcggccgttc tcgatgcaga catcggagac gtccccgcgc cggccgtgcc
     8101 tcggatccca gaccgatccg ttgcggatgc ggatcgtccc gaccggcgcg tcctccttcc
     8161 gggtcatgcg gccgctcgca gccggtcgag aagcaccgcg agaatgtccg cttccgaagg
     8221 aagcgccacc gccgatgccg gcggggccgg agcggcggag accgccgagg gga
//
LOCUS       DASRIQ010000394         6326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3829898, whole genome shotgun sequence.
ACCESSION   DASRIQ010000394 DASRIQ010000000
VERSION     DASRIQ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3829898"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..953)
                     /locus_tag="VFD06_09665"
     CDS             complement(75..953)
                     /locus_tag="VFD06_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZN03837.1"
                     /translation="MNRPSQSDVGALESVLLKHPRDAFRDAATVEAAWRGLNYLAPPD
                     PGRAAAEYDRFAARIEETGARIHWMPPDNVTGLDSLYVRDASIPSDAGMILCNMGKAA
                     RSTEPAAQAEAFRRLGIPVAGAVTGEGKIEGGDVVWLDPTTLAVGRGYRTNDEGIRQL
                     RGILGEGIDVVVAHLPHWRGPADVFHLMSILSPIDRDLALVYSPLLPVPFRELLLSRG
                     FGLVEVPDEEFESMGCNVLALGPRRCLMLDGNPVTRRRLEQARSQVAVFSGEEICRKG
                     SGGPTCLARPILRAWR"
     gene            1091..2965
                     /locus_tag="VFD06_09670"
     CDS             1091..2965
                     /locus_tag="VFD06_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN03838.1"
                     /translation="MIGRRQFLAAFSGIGLGSTLLPGTLWALAQGKPAITSEMIKEAE
                     AIAGVALPDDLRAMMLGNLNDQAKGYQAIYALHLPNAVEPCLLFDPLLPGMSQEIEKG
                     PGRMSAAPAVAARGVPKDLEEVAFWSVRALAELVRTRKVSAVALTDMYLARLKRYDPL
                     LKFNVTLTEDRARAQAQECDRDQAAGRYRGPLHGLPWGAKDLLAVKGYPTTWGAGGFE
                     HQAIDEDATVVKRLDAAGAVLVSKLTLGALAMGDVWYGGTTRNPWNPSQGSSGSSAGP
                     ASATAAGCVAFSIGSETLGSIASPSTRCGCTGLRPTFGRVPRTGAMALSWSMDKLGPI
                     GRAVEDCALVLESIAGPDGQDRTVRGGAFVWNADLDWRRLRVGYLKADFEPPSKPASD
                     AATPAPGGGETPPAPLTPQQAAQAERDRVRRQARGVRAEYDRRFGQAALAKLRGMGID
                     LVPVTLPELPYEPMVSMLSAEAAAAFDELTRSGRDRLLTAQGPDDWPNAFRSARFIPA
                     VEYIQASRARRLAMEQVAKAVENVDVVVAPTSSTQLVVTNLTGHPALILPNGLRGDDA
                     PKPPSNVALEDNVGGPGTPVSLTFIGRLYGEAKLLALARAYQEATKFHLARPPLPPRA
                     "
     gene            2995..4380
                     /locus_tag="VFD06_09675"
     CDS             2995..4380
                     /locus_tag="VFD06_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN03839.1"
                     /translation="MPAHALLRRDLGTLEAYAALIGILIGAGIFKVTSQSWALTGPSV
                     ILAHLVLAVPVLATSVPYCVFLSTPLGLEPGGEYAHISRTFGGRGVAFVGAWLKCISY
                     IGALAFLAATFADYLGGLAPGRLDGASARTATALVSLVLVYAIHVVGVRWFGRLQVAM
                     LAVLGLSIVVLVVPGIFAVHLSHYRPFFTHGLPGFAAAMPPLFFAYAGFESLAQAAGE
                     VRDSTRRLPRVFLLGIAGTTVLYLLMSVVTFGVLPGARLQASAAPMTEVAAVYLPGGA
                     ALFVTLGALMAIATSLNSTMLVPSRLAVVLAEDGLVPGWIGAVSPRTGTPVAALTLTF
                     AAAVLLLVGGQIALGLNIAVFALVGLYLLHSLALLLLPAMNPALFRTVTVSIPLGAQR
                     AAAVVSMVAMGALFLLQVKGDLGTIASRSFGERIAGGSLTAVELSVVWAGVGALLYAV
                     GRRRAAGLPEG"
     gene            complement(4335..5093)
                     /locus_tag="VFD06_09680"
     CDS             complement(4335..5093)
                     /locus_tag="VFD06_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN03840.1"
                     /translation="MTLDAGCRVANLRQGEPEVHGTLRVWTRFGRAEGARALSLRILE
                     IAPGLSPGLLNADCDEVLYVIEGTGTAYLDGHAFAIGPDEGLHLKPGVLLALENPGSH
                     PLTLASARCPDDGTPAIFEAARIRPKVGTLPPAAVARLGDCPPETASDGRSFQVLIDA
                     SGSGAPVTQFVGSIPPGRAPDHFHEYEEVLVVLEGHGRVWAGGESAPVRPGTCVFLPR
                     RQIHCVENLGEERLRLMGVFYPSGSPAARRLPTA"
     gene            5137..6216
                     /locus_tag="VFD06_09685"
     CDS             5137..6216
                     /locus_tag="VFD06_09685"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZN03841.1"
                     /translation="MDDKKNHGVEATNQEYARYDQVRKEQRYHLSDATDSSDAYDEEY
                     RIRTVDIGRFLEGDAADRRAFARDLGEALRGIGFAILEGHPVDARLYDEAAERIEEFY
                     TSVPLEEKLRFRASRHGSVNQGYFPIRETSDIHPDLVEGWVFCRRAFALDAGRPVRLE
                     EFWPRQEMEPFFQRLCLEHEALILPVMRSILMDLGCDPHLYDARLTGTNFGLRLNYYP
                     PLSEKDEASGAARILGHEDVDLFTFLPAPVIEGLQVLNRASMKWIRLNAPRGTIILNT
                     GDYMQRITNDILPSTTHRVVKPRDPALRGRARLSFPMAVYVWEDEVLEVLPGLGEPRY
                     APIKAVQFHTRTTSKFYGDDYAEPE"
BASE COUNT          889 a         2267 c         2173 g          997 t
ORIGIN      
        1 tcactccagg agtgcggcgc cttccgggtt ggcgactcca tctccaatat atctccaatc
       61 gttcgcgtaa cctttcaccg ccaggcacgc aggatcggcc gcgcgaggca ggtggggcct
      121 cccgatccct tcctgcagat ctcctcgccg gagaagaccg cgacctgcga cctcgcctgc
      181 tccagacgcc tccgagtgac ggggttgccg tcgagcatga ggcagcggcg cgggccgagg
      241 gccaggacgt tacatcccat gctctcgaac tcctcgtccg gtacttcgac gaggccgaat
      301 ccgcgcgaga gcaaaagctc gcggaacggg accggcagca ggggagagta gaccagcgcc
      361 aggtcgcgat cgatcgggct gaggatcgac atgagatgga agacgtcggc ggggccgcgc
      421 cagtggggca gatgggccac gacgacatcg atcccctcgc cgaggatccc ccgcagctgg
      481 cggatgcctt cgtcgttggt gcggtagccg cgccccacgg ccagcgtcgt cgggtcgagc
      541 cagacgacgt cccctccttc gatcttcccc tctccggtga cggcgccggc cacggggatc
      601 ccgagcctgc ggaaggcctc ggcctgggcc gccggctccg tcgagcgcgc cgccttgccc
      661 atgttgcaca ggatcatccc cgcgtccgac gggatggagg cgtcgcgtac atataaggag
      721 tcgaggccgg tgacgttgtc gggcggcatc cagtggatgc gggcccccgt ttcctcgatc
      781 cgcgccgcga agcggtcgta ctcggcggcg gcgcggccgg gatcgggcgg cgccaggtag
      841 ttcagccctc gccacgccgc ctccaccgtc gccgcgtcgc gaaaggcatc gcgcgggtgc
      901 ttcagcagga cgctctcgag cgcgccgacg tcggactgcg aaggccggtt cacacaacac
      961 ctcccgcctg cgcttcatca aatcccgtgg agttcacgag gaatcgtgat gctaccactg
     1021 gtcacctggt ggcgccggca gtgtcaccta tacttcggcc gctcgcctgt tcgctggagg
     1081 ttctcacgac atgatcggtc gccgccagtt cctcgccgcc ttctccggaa tcggactcgg
     1141 ctcgacgctc ctgcccggga cgctctgggc cctggcgcag gggaagcccg ccatcaccag
     1201 cgagatgatc aaggaggccg aggcgatcgc cggagtcgcc ctccccgacg acctgcgcgc
     1261 gatgatgctc ggcaacctga acgatcaggc gaaggggtac caagcgatct acgccctgca
     1321 cctgcccaac gcggtcgagc cctgtctgct gttcgatcct ctgctgccgg ggatgtcgca
     1381 ggagatcgag aaggggcccg gcaggatgtc cgcggcgccc gcggtcgcgg cgcgcggcgt
     1441 cccgaaagat ctggaggagg tcgccttctg gagcgtgcgc gctctcgccg agctggtgcg
     1501 cacgaggaag gtctcggccg tggcgctgac cgacatgtac ctggcgcggc tcaagcgcta
     1561 cgacccgctc ctgaagttca acgtgaccct gacggaggat cgggcccgcg cgcaggcgca
     1621 ggaatgcgac cgcgatcagg cggcggggcg ttaccgcggc ccgctgcacg ggctgccgtg
     1681 gggcgcgaag gacctgctcg cggtgaaggg ttacccgacg acctgggggg cgggcgggtt
     1741 cgagcatcag gcgatcgacg aggacgcgac ggtcgtgaag cgcctcgacg ccgcgggcgc
     1801 ggtcctggtg tccaagttga cgctgggggc gctggcgatg ggggacgtct ggtacggggg
     1861 cacgacacgc aatccctgga acccttcgca gggatcgagc ggctcgtcgg ccgggccggc
     1921 ctccgccacc gccgccggct gcgtcgcctt cagcatcggc tccgagacgc tcggttcgat
     1981 cgcctctccc tcgacgcgct gcgggtgcac cgggctgcgc ccgaccttcg gccgggtgcc
     2041 gcgcaccggt gcgatggccc tgtcgtggag catggacaag ctcgggccga tcggccgcgc
     2101 ggtggaggac tgcgccctcg tgctcgagag catcgccggt ccggacggtc aggaccggac
     2161 ggtgcgcggc ggcgccttcg tctggaacgc cgacctcgac tggcgacgtc tccgggtcgg
     2221 ttatctcaag gccgatttcg agcctccttc caagccagcc agtgacgcgg cgacacccgc
     2281 gccgggaggt ggcgagaccc cgccggcgcc gctgacgccg cagcaggcgg cgcaggccga
     2341 gcgcgaccgt gtccgccgcc aggcgcgcgg cgtgcgcgcc gagtacgatc ggcgtttcgg
     2401 ccaggcggcg ctcgcgaagc tgcgcggcat ggggatcgat ctcgtcccag tcacgctccc
     2461 cgagctgccg tacgagccga tggtcagcat gctcagcgcg gaagcggcgg cggcgttcga
     2521 cgagctgacg cgctcggggc gcgaccggct gctgacggcg caggggccgg acgactggcc
     2581 gaacgcgttc cggtcggcgc gcttcatccc ggcggtggag tacatccagg cgagccgggc
     2641 gcggcgcctg gcgatggagc aggtggcgaa ggcggtcgag aacgtcgacg tcgtcgtcgc
     2701 gccgacctcc tcgacgcagc tcgtcgtcac caacctcacc ggccaccccg cgctcatcct
     2761 gccgaacggc ctgcgcggcg acgacgctcc gaagccgcct tcgaacgtcg cgctcgaaga
     2821 caacgtcggc ggcccgggca cgccggtcag cctgaccttc atcggccgcc tctacggcga
     2881 ggcgaagctc ctggcgctgg cgcgggccta ccaggaagcg accaaattcc acctggcgcg
     2941 gccgccgttg ccgccgcgcg cctgaaccgc gctgcgtcac cgtcgcgtcg gcctgtgccg
     3001 gcgcacgccc tcctccgccg ggatctgggg acgctcgagg cctatgcggc gctgatcggc
     3061 attctgatcg gcgccggcat cttcaaggtg acgagccagt catgggcgct caccggcccg
     3121 agcgtcatcc tcgcccacct cgtcctggcc gtgcccgtcc tcgccacctc cgtcccgtac
     3181 tgcgtcttcc tctccacccc cctcggcctc gagcccggcg gcgagtacgc ccacatctcg
     3241 aggacgttcg gcggccgcgg cgtcgccttc gtcggcgcgt ggctcaagtg catctcctac
     3301 atcggcgccc tcgccttcct ggccgccacc ttcgccgatt acctgggcgg actcgccccc
     3361 ggcaggctcg atggagcatc cgcccgtacg gcgacggccc tggtcagcct cgtcctcgtc
     3421 tatgccatcc acgtcgtcgg ggtgcgatgg ttcgggcgcc tccaggtggc gatgctggcg
     3481 gtgctcggtc tgtcgatcgt ggtcctcgtc gtaccgggga tcttcgccgt ccatctgtcc
     3541 cactaccgcc ccttcttcac gcacggtctc ccgggtttcg ccgcggcgat gccgccgctc
     3601 ttcttcgcct acgcggggtt cgagtccctg gcgcaggcgg cgggcgaggt ccgggacagc
     3661 acgcgccggc ttccccgcgt cttcctcctg ggaatcgcgg ggacgacggt tctctacctc
     3721 ctgatgtccg tcgtgacgtt cggtgtcctt ccgggcgccc gcctgcaggc gagcgccgct
     3781 ccgatgactg aggtcgcggc ggtctacctt ccgggcggcg cggccctctt cgtgacgctg
     3841 ggggcgctga tggcgatcgc cacctccctg aactccacga tgctggtccc ctcgcgtctc
     3901 gccgtcgttc tcgccgagga cggcctcgtg cccggatgga tcggcgcggt gtcgccacgc
     3961 accggaaccc ccgtcgcggc cctgacgctg acgttcgccg ccgccgtgct cctcctcgtg
     4021 ggaggtcaga tcgcgctcgg gctgaacatc gccgtgttcg ccctggtggg gctctacctc
     4081 ctccacagcc tcgcgcttct gcttctcccg gccatgaacc cggccctctt caggaccgtc
     4141 accgtctcga tcccgctcgg cgctcagagg gccgccgccg tggtctcgat ggtcgcgatg
     4201 ggagcgctct tcctcctcca ggtgaaaggc gatctcggga cgatcgcctc ccggagcttc
     4261 ggcgagagga tcgctggagg ctctctcacc gccgtggagc tttcggtggt gtgggcgggc
     4321 gtgggggcgc tgctctacgc cgtgggaaga cggcgggcgg cggggctgcc cgagggatag
     4381 aagaccccca tcaggcgcag ccgttcttcc ccgaggttct cgacgcagtg gatctgccgc
     4441 cgggggagaa agacgcaggt accgggtcgg acgggggcgc tttcgccccc ggcccagacc
     4501 cgaccatgcc cctccaggac gaccagcacc tcctcgtact cgtggaagtg gtccggtgcg
     4561 cggccgggcg gaatcgagcc gacgaactgc gtgaccggcg cgccggagcc gctcgcgtcg
     4621 atcaggacct ggaacgagcg tccgtcggag gccgtctccg gcggacaatc gccgagcctg
     4681 gcgacggcgg ccggcgggag cgtgccaacc ttcggccgga tccgggccgc ttcgaagatc
     4741 gccggcgtcc cgtcgtcggg gcatcgtgcg ctcgcgagcg tgagcggatg cgaccccggg
     4801 ttctcgagag ccagcaggac accgggcttc agatgtagac cttcatccgg cccgatggcg
     4861 aaggcgtgcc cgtccaggta cgcggttccg gttccttcga tcacgtagag cacctcgtcg
     4921 cagtccgcgt tgaggagccc gggggagagg ccgggtgcga tctccaggat ccggagggac
     4981 agggcgcggg cgccctccgc ccgtccgaac ctggtccaga ccctcagggt accgtggacc
     5041 tcgggctcgc cctggcgcag attcgcgacg cggcaccccg cgtcgagcgt cacgtccact
     5101 tgtcgttccc ccttcgcggt ggattatcct acaggggtgg acgacaagaa gaatcacggc
     5161 gtggaggcca ccaaccagga gtacgcgcgc tacgaccagg tccgcaagga gcagcgctac
     5221 cacctctccg acgcgaccga ctcctccgac gcctatgacg aggagtaccg gatccggacc
     5281 gtcgacatcg ggcgcttcct cgagggggac gccgccgacc ggcgggcgtt cgcgcgcgat
     5341 ctgggcgagg cgctccgcgg gatcggcttc gccatcctgg aggggcatcc ggtcgacgcg
     5401 cggctctacg acgaggcggc ggagcggatc gaagagttct acacgtcggt ccctctcgag
     5461 gagaagctgc gcttcagggc ttcgcggcac ggctcggtga atcaaggcta tttcccgatc
     5521 cgggagacca gcgacatcca cccggacctg gtggagggct gggtcttctg ccggcgcgcc
     5581 ttcgccctcg acgccggccg ccccgtgcgc ctggaggagt tctggccccg ccaggagatg
     5641 gagccgttct tccagcgact ctgcctcgag cacgaggcgc tgatcctgcc cgtcatgcgg
     5701 agcatcctca tggatctggg ctgcgatccg cacctgtacg atgcgcgttt gaccggcacg
     5761 aacttcggct tgcgcctgaa ctactacccg ccgctctccg agaaggacga agcctcaggg
     5821 gccgcccgca ttctcggcca cgaggacgtg gacctgttca cgttcctgcc ggcccccgtc
     5881 atcgaggggc tccaggtcct caaccgcgcc agcatgaagt ggatccggct gaacgcgcct
     5941 cgcgggacga tcatcctgaa caccggtgac tacatgcagc ggatcacgaa cgacatcctg
     6001 ccgtccacga cgcaccgggt ggtgaagccg cgcgatccgg ccctccgcgg acgggcgagg
     6061 ctctccttcc ccatggccgt gtacgtgtgg gaggacgagg tcctcgaggt ccttcccggt
     6121 ctcggcgagc cacgctacgc gccgatcaag gccgtccagt tccacacgcg caccaccagc
     6181 aagttctacg gcgacgacta cgcggagccg gagtagaggc gagcggggca ggccagcgga
     6241 ggcgggggcg actgcgggcc tcccgatccc ttccggcaga tctcctcacc cgagaagacc
     6301 gcgacgtgcg ctcccgcctg ctccac
//
LOCUS       DASRIQ010000395         4804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3273945, whole genome shotgun sequence.
ACCESSION   DASRIQ010000395 DASRIQ010000000
VERSION     DASRIQ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3273945"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2840
                     /locus_tag="VFD06_09690"
     CDS             <1..2840
                     /locus_tag="VFD06_09690"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN03842.1"
                     /translation="RRAWPYRDLPRDRFDQVIAMLSEGVATSRGRRGALLHRDGVHRR
                     LRARRGTRLLALTSGGAIPDNADYDVVKDPEGVFVGRINEDFAIESMAGDVFLLGNAA
                     WQIRRVERGRVRVEAAPGATPTVPFWLGEAPARTRELSAAVGALREEVDRRGASAGRF
                     LQEEAGIDEEPARQIVEFIAEGKRVLGVVPASGRIVAERFFDETGGMQLVIHAPLGGR
                     INRAWGLALRKRFCRTFDFELQAAATDDGINLSLGPQHSFPLQDVFHFVRARGVEEVL
                     TQAVLPSPIFAARWRWTVTRSLEVPRWEGGRKVPPPLLRMRCDDLLAAVFPAQAACQD
                     NAPGGDIPIPDHPLVFETLRDCLEEALDVEGLRGVLQEIEAGGIEILARDTPQPSVFA
                     HALLNAMPYAFLDDAPLEERRARAVNLRRALPEAGDLGRLDPAAIRTASEDAWPRPRD
                     AEELHEALLGLTLLPLEVVEGRFEASAASWLEDLEADARVVRLRGEGSAIFVASAEAE
                     REARAALTGDAEAIQRVVRGWAEVSGPFTPASLARALCLAEGDVATACAALEGEGTLL
                     RGSFTEAATAGAQAEEWCDRRILARIHRATLQTLRREIEPVPPAVFLRFLAAWQHAAP
                     GTAVRGAEGLLEVIELLQGYEAPLAAWESALLPARVERYDPADLDRLLLGGQVVWGRF
                     AARPDGGGGMTRASLVSLGLREDLPWLLDRDGPPPTLSSRARALEEFLRARGPSYASE
                     AARVLRLLPSEIEDAMGELIAAGLATLDGFAALRRIASASSTRRSVPGRRAGRFVRRR
                     GSGTESGDGRLWLFGRDAPAPEDRMTQAAAQLLRRWGVLFRDLLAREGTAPPWRDLLT
                     ELRRAEARGEVRGGRFVDGFPGEQFALAEAVDALRAARRQGPGDAVREISAADPLNLA
                     GILTPGPRIPAQGRGRVLLHDGVPAAGADAR"
     gene            2840..3949
                     /locus_tag="VFD06_09695"
     CDS             2840..3949
                     /locus_tag="VFD06_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZN03843.1"
                     /translation="MTADPRQARWLALLAVTGIALLLCWRMMQPFLEVIAWATVLAVV
                     FFPAYRGLRTWIRRPWVASALAVLLVVATVLAPLVFVSTAVLNELQGMAASVKESLDR
                     LRSDPRAAAELQSRLDRIRGTIDVESVLSEENVSAFATRASQWLLERSAGLVGGLLSI
                     LASFGFAIFTLFYLFRDGETVVARLPSVLPLDRSRAEALLRRAHEIITASVYGVVIIA
                     VIQGTLGGIMFAILGLPSPILWGVVMTFLSTLPMVGSGLVWGPAAVVLAVTGQWGKMA
                     ALVLWGALVIGTVDNFLRPRLVGSRTRMHDLFIFFSVLGGLAAFGIIGLLLGPVILGV
                     TFVLLDAVFGDAMGARPAAAAGATPVSAPAARKPV"
     gene            complement(3960..>4804)
                     /locus_tag="VFD06_09700"
     CDS             complement(3960..>4804)
                     /locus_tag="VFD06_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZN03844.1"
                     /translation="LRVVYPLDKHTTYRYSGNGKSDNASFWGMFDSGSSTVEYAGRRV
                     KISGKSGVALYADIDIQVPARLGQGLLRNYVGRMEGKGLEGTLTFDTASGDMDLADLK
                     GTIGADTGSGDVHITDVKGSLDCDTGSGNVTVEGFEGDLLKGDVGSGDISIRAGSIGR
                     VDLDTGSGDVRLKAAEVQELKADTGSGDVTFDIGGGSLKRASFSTGSGDVTLRLGPDA
                     SFEARVDQGSGDMDNRFGDAVPIVQGREVIGYKRGDGRAKIDVETGSGDFLLEPGSAS
                     ASRR"
BASE COUNT          532 a         1737 c         1798 g          737 t
ORIGIN      
        1 tccggcgcgc ctggccgtac cgcgacctgc cgcgcgaccg cttcgaccag gtgatcgcca
       61 tgctgtccga gggggtcgcg acgagccggg gacgccgcgg ggcgctcctg caccgcgatg
      121 gcgtgcatcg ccggctgcgg gcgcggcgcg gcacgcggct cctggcgctg acgagcggcg
      181 gcgccattcc ggacaacgcc gactacgacg tggtcaagga cccggagggg gtgttcgtcg
      241 gccggatcaa cgaggacttc gccatcgaga gcatggcggg tgacgtgttc ctgctcggca
      301 acgccgcctg gcagatccgc cgcgtcgagc gcggcagggt gcgggtcgaa gccgcccctg
      361 gtgcgacgcc gaccgtgccg ttctggctcg gcgaggcgcc ggcccggacg cgtgagctgt
      421 cggctgccgt cggcgctctg cgcgaggagg tggatcggcg cggcgcttcg gccgggcgct
      481 tcctccagga ggaggcgggg atcgacgagg agccggcgcg gcagattgtc gagttcatcg
      541 ccgaggggaa gcgggtgctc ggcgtcgtcc cggcctccgg ccggatcgtc gcggaacgct
      601 tcttcgacga gaccggcggc atgcagctgg tgatccatgc gccgctcggc gggcggatca
      661 accgcgcctg ggggctggcg ctccgcaagc gcttctgccg caccttcgac ttcgagctgc
      721 aggcggcggc gaccgacgac ggcatcaacc tgtcgctcgg cccgcagcac tcgttcccgc
      781 tccaggacgt gttccacttc gtgcgggcgc gcggggtgga ggaggtcctg acgcaggcgg
      841 tgctgccgtc gccgatcttc gccgcgcgct ggcgctggac cgtgacccgc tccctggagg
      901 tgccgcgctg ggaagggggg cgcaaggtgc cgccgccgct cctgaggatg cgctgcgacg
      961 acctgctggc cgcggtcttc ccggcccagg ccgcctgcca ggacaacgcc cccggcggcg
     1021 acatcccgat cccggatcac ccgctggtgt tcgagacgct gcgcgactgc ctcgaggagg
     1081 cgctcgacgt cgagggtctg cgcggtgtcc tgcaggagat cgaggcgggg gggatcgaaa
     1141 tcctggcgcg cgacacgccg cagccgtcgg tcttcgccca cgcgctcctc aacgccatgc
     1201 cgtacgcttt cctcgacgat gcgccgctcg aggagcggcg ggcccgcgct gtcaacctcc
     1261 gacgggccct gccggaggcg ggggatctcg gccggctgga tccggcggcg atccggacgg
     1321 cgtcggaaga cgcctggccg cggccgcgcg acgccgaaga gctgcacgaa gcgctcctcg
     1381 gcctgacgct cctccctctc gaggtcgtgg aggggcgctt cgaggcgtcc gccgcgtcct
     1441 ggctcgagga tctcgaggcg gacgcgcgtg tcgtccgcct gcgcggcgaa ggctccgcca
     1501 tcttcgtcgc ctccgccgaa gcggagcggg aggcgcgcgc cgccctgacc ggggacgcgg
     1561 aggcgatcca gcgcgtcgtg cgcggctggg ccgaggtctc ggggccgttc acgccggcga
     1621 gcctggcgcg cgctctctgc ctcgccgagg gggacgtggc gacggcctgc gcggcgctcg
     1681 aaggggaggg gacgctcctg cgcggttcct tcaccgaggc agcgacggcg ggcgcgcagg
     1741 ccgaggagtg gtgcgaccgg cgcatcctgg cccgcatcca ccgcgccact ctccagacgc
     1801 tgcggcgcga gatcgagccg gtcccccccg cggtcttcct gcgcttcctc gccgcctggc
     1861 agcacgccgc cccggggacg gcggtgcgcg gcgccgaagg actcctcgag gtcatcgagc
     1921 tgctgcaggg gtacgaggcg ccgctcgcgg cgtgggagtc ggccctgctg ccggcgcggg
     1981 tcgagcgcta cgacccggcc gacctcgatc ggctgctcct cggcggacag gtcgtctggg
     2041 ggcggttcgc ggcccgcccc gacggcgggg gagggatgac gcgcgccagc ctcgtcagcc
     2101 tgggtctgcg cgaggacctg ccgtggctcc tcgatcgcga cggtccgcct ccgacgctgt
     2161 cgtcgcgcgc ccgcgcgctg gaagagttcc tccgcgcgcg cggtccgtcg tacgcctcgg
     2221 aggcggcccg ggtgctgcga ctcctccctt cggagatcga ggacgcgatg ggggagctga
     2281 tcgccgccgg gctggcgacg ctcgacggct tcgcggcgct gcgccggatt gcttccgctt
     2341 cctccacccg ccgctccgtg ccggggcggc gcgccgggcg tttcgtccgc cgccgcggca
     2401 gcgggacgga atcgggggac ggccgcctgt ggctcttcgg gcgggacgca ccggcgccgg
     2461 aggatcgcat gacgcaggcg gcggcacagc tcctgcgccg ctggggggtg ctgttccgcg
     2521 acctcctggc ccgcgagggg accgcaccgc cgtggcgcga cctcctgacc gagctgcgcc
     2581 gcgccgaggc gcgcggcgag gtgcgcggcg ggcgcttcgt ggacgggttc cccggagagc
     2641 agttcgcgct cgcggaggcg gtggacgcgc tgcgggccgc ccggcgccag ggtcccgggg
     2701 acgcggtgcg ggagatctcc gccgccgatc cgctgaacct cgccggcatc ctcacccccg
     2761 ggccgcgcat cccggcgcag gggcgcggcc gcgtgctgct ccacgacggc gtcccggccg
     2821 cgggggcgga cgcccgatga tgaccgccga tccccgccag gcgcgctggc tggcgctgct
     2881 cgccgtcacc ggcatcgccc tcctcctctg ctggcggatg atgcagccgt tcctcgaggt
     2941 gatcgcctgg gcgacggtgc tggcggtggt gttcttcccc gcctatcgcg gcctgcgcac
     3001 ctggatccgg cgcccgtggg tggcgtcggc cctggcggtg ctcctcgtcg tcgcgacggt
     3061 gctcgcgccg ctcgtcttcg tcagcaccgc ggtcctcaac gagctgcagg ggatggcggc
     3121 ttcggtcaag gagagcctgg atcggctgcg atccgatccg cgggcggcgg ccgagctgca
     3181 gagccgactg gaccggatcc gtggaaccat cgacgtcgag tcggtcctgt cggaggagaa
     3241 cgtctccgcc ttcgccaccc gcgccagcca gtggctcctg gagcgttccg ccgggctcgt
     3301 cggcggcctc ctgagcatcc tcgcgagctt cggcttcgcc atcttcaccc tcttctatct
     3361 cttccgggac ggcgagacgg tcgtggcgcg gctgccgtcc gtcctgccgc tcgatcgatc
     3421 gcgggccgag gcgctcctgc gccgggcgca cgagatcatc accgccagcg tctacggcgt
     3481 cgtcatcatc gccgtcatcc agggaacgct cggcgggatc atgttcgcga tcctcggcct
     3541 gccgtcgccg atcctctggg gggtggtgat gacgttcctc agcaccctgc cgatggtcgg
     3601 gtcggggctc gtgtgggggc cggcggcggt cgtgctggcg gtgaccggtc agtgggggaa
     3661 gatggcggcg ctcgtgctgt ggggggcgct cgttatcggc acggtcgaca acttcctgcg
     3721 gccgcggctc gtcggcagcc gcacccgcat gcacgacctg ttcatcttct tctcggtgct
     3781 cggggggctg gcggcgttcg gcatcatcgg cctgctcctc gggccggtga tcctcggggt
     3841 gacgttcgtt ctgctcgacg cggttttcgg cgacgcgatg ggcgcccggc ccgcggccgc
     3901 ggcgggggcg acgccggtct cggcccccgc cgcccgcaag ccggtctgaa aagggcggcc
     3961 tagcggcgcg acgcggaggc gctgccgggc tcgagcagga agtcgccgct gccggtttcc
     4021 acgtcgatct tcgcccgtcc gtcgccgcgc ttgtagccga tgacctcgcg cccctggacg
     4081 atcgggacgg cgtcgccgaa gcggttgtcc atgtcgccgc ttccctggtc gacgcgcgcc
     4141 tcgaacgacg cgtccgggcc gagcctcagc gtgacgtcgc cgctgccggt ggagaaactc
     4201 gcgcgcttca ggctgccgcc gccgatgtcg aaggtcacgt ccccggagcc ggtgtcggcc
     4261 ttgagctcct gcacctcggc cgccttgagg cggacgtcac ccgacccggt gtcgagatcg
     4321 acgcggccga tcgacccggc gcggatcgag atgtcgccgg atccgacgtc ccccttgagg
     4381 aggtccccct cgaacccttc gacggtgacg ttgcccgagc cggtgtcgca gtcgagggag
     4441 cccttcacgt cggtgatgtg gacgtccccc gaaccggtgt cggcgccgat cgtccccttg
     4501 aggtccgcga ggtccatgtc gccgctagcg gtgtcgaagg tgagcgtccc ctcgagcccc
     4561 ttcccctcca tccgccccac gtagttccgc agcagcccct ggccgaggcg ggccggcacc
     4621 tggatgtcga tgtccgcgta cagggcgacg ccgctcttcc ccgagatctt cacccgccgt
     4681 ccggcgtact cgaccgtgct gctgccgctg tcgaacatgc cccagaacga tgcgttgtcc
     4741 gacttgccgt tccccgaata gcgataggtg gtgtgcttgt cgaggggata gacgacccgc
     4801 agcg
//
LOCUS       DASRIQ010000396         9387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_166243, whole genome shotgun sequence.
ACCESSION   DASRIQ010000396 DASRIQ010000000
VERSION     DASRIQ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166243"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="VFD06_09705"
     CDS             <1..1750
                     /locus_tag="VFD06_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03845.1"
                     /translation="WGGPDLFGYVFTDSDEPDGPVFAWRDISASGRTAILTGEDALSA
                     AIPLAFLFPFYGRTFTSVRLSSNGYLTFGDVKAPYENQPLPSPGISSAMIAGYWDNLI
                     VPGPQNAIWLSELHTFTVQYDGVLRMRGGGPYTFQIVLFDSGEILFQYLEMVGLGYSE
                     TIGIQDASGTAGLPIAFHSAYVHDRLAVRLLMQKDWVTAAPGSGRLRSFESESVALTF
                     DASGLAAGTYEGRLPILTNDPDRRSVEIPLMLSVEGARAIAAVPAAVDFGSVFAADGS
                     RLDLTIVNSGSLPVTVTALVPGDPSVITDFAPFLLAPGGHRLLTLQWLPAEPGILLAD
                     LRIESDATNAPSLSVPLTGIALNIPPRAAAIWPPAQVECAGPDGSDVVLDASPSGDDD
                     APPGEPTGIALYEWIENPRTAVEELLGTGMRIARRLPPGTHRLALRVTDSRGAIDSVE
                     STVTVADTTPPELSVRASPTFLWPPHHRMVPVRLILSTRDACSDEVTMRLTEISSSEP
                     DDAPGNADGRTTGDIAGVEAGTPDTRIQLRAERSVPGTGRVYTIRYVATDAAGHKTIR
                     EAVVTVPANKVSARRR"
     gene            1875..2681
                     /locus_tag="VFD06_09710"
     CDS             1875..2681
                     /locus_tag="VFD06_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN03846.1"
                     /translation="MPLSLGSQRSRSIAVDGFRVTEVWFPPFLRLPPHTHDRASLAVM
                     LGGSFDLTLSRSSHSCPPASVFTEPVGERHGNRIDAAGAHVIVVQPDPARADLTRPCR
                     DLFDRPAHFRHAAITACARQLGAELQARDAVTPLAIEGTVLSMIASAARLTEIAPRRA
                     PAWLDRTIELLHARFLDTLTTAGVAHEVGIHPVHLARVFRARHGMTMGAYVRRLRLDW
                     AARRLTRSRDPLCDIAVAAGFADQSHFTRAFKRHLGDTPHRFRRRLAPRR"
     gene            2814..3461
                     /locus_tag="VFD06_09715"
     CDS             2814..3461
                     /locus_tag="VFD06_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03847.1"
                     /translation="MRTKTWLTVVVLVVSGAAGASALAARGGAVRDDSIETAGASAAA
                     AANAGHLLTATGGYHFTIPADFNGGIFGTLTDIKNRVTFNAKKDADGSVSGWWTYEQE
                     VDGGVFKFGGPVTCLNIYDTPVLTRTPDIPPMTQNRAKWGGRVEKSNDETIPVGRFMW
                     FQSIDNGEGANGYSDVSTLVGLGDEAANIAFCNADRVPNSNFGPHRIGGGNIQVD"
     gene            complement(3517..3738)
                     /locus_tag="VFD06_09720"
     CDS             complement(3517..3738)
                     /locus_tag="VFD06_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03848.1"
                     /translation="MPKRPTYAASIPPGLLVRVLCRGDCQALRYAEVSKYPWTPNDPE
                     LYATCLKCGYQARDSYNWGRKRERPERPE"
     gene            3927..>9387
                     /locus_tag="VFD06_09725"
     CDS             3927..>9387
                     /locus_tag="VFD06_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN03849.1"
                     /translation="MSLALILAAGAAAPGAPKRSPPGPHGPKNPQYVEGEILVKFRPQ
                     APVARRGALRSEVAGAVKSRFMSGAELLRLGAGVGVGQALARLKGAPEVEYAEPNYIL
                     HATRTPDDPFFAQQWSLLNTGQAGGAAGSDIDATAAWDISIGGASVVIAIVDTGIDLD
                     HPDLAPNLWTNPDEIPGNLVDDDGNGRIDDVHGWDFVNHDDDPDDDLGHGTHVAGTIA
                     GAGDNGIGITGVAWRASLMPLKFLDRSGAGTTSDAIQAIDYAASEGARILNASWGGGG
                     FSMAMLESIRDAAAREVLFVAAAGNDAADTDIAPFFPAGYDAPNVVAVAASDRSDRLA
                     LFSSFGETTVDLAAPGVDILSTLPGGTYGFSSGTSMATPHVSGVAALVLGFAPGVGAE
                     SLRRRLLDEAEPIAALEGKVAVAGRLNAFECLSGADAVRPGPIDDLSVVEPLSDGLVL
                     AWTATGDDGDEGAAAGYDLRISEDPFDLLGFEAAPRFHLPGPPLPAGTPETKEIGGLD
                     PSRTYYLGVRAIDEWGNAGPPGFATASTLQPPAMEVTPETSSAALLSGQTATTVVTLR
                     NASAGTLDWSIERPILRPTAVRAGPRTERWGGPDAFGYVFTDSDEPDGPVFDWKEVPA
                     DGRSALVAGDDLLSAPIPLGFPFPFYGRTFASVRIASNGFLSFTHEDAPYENQPLPSP
                     GAPGNLIAPFWDDLFVWGLQDVIWLAEPDTFTVQYNNVLRYGGGGPYTFQVVLFASGE
                     ILFQYLSMTDTLDSATIGIQDESGTAGLLIAFNAPYVHDRLAIRLAIQEEWLSASPRS
                     GRLRRGESAPITLSFDASGLGGGLHEGWLPIVGNDPDRPRLEHPVLLTVEDAPGIAVE
                     PAAVDFGRAFQGYASRRSVEILSSGSLPLTVHAAVPDDPRVGTDFAPFVLAPGERRLL
                     AIDWVPAAIGDLQANLRIDSAARNVPSLTVPLAGSAVAPPVLRIEPETIDETVPLGGA
                     SERVLSLANDGATDLDYTIELDRPGELSAGAFDSFPPSPEPLTCVVADPSAGMLYGQE
                     RRGRRFYRLRVRDQQWETLPPSPNSSGANCGAALLAGRIYTASSDPPAVLGVYDTAFG
                     SWTTLPSPLGAATANIASDGDRWLYLALDRTVKRFDPAVGGAGESLPVPPFPFDRWGG
                     LQVHAGLLYGHVGNSSTWFGRYDPFVRIWSMLPFLPLGGTSGAAIDPVSGDYFAAGPA
                     GGANLYRFDSKNSVWSTMPLPFPVEDGGLAWLGLPVGAIYFVEGERGTRVGRLLLGDA
                     RAAAVASASGRVAPGGTGEVRLQFDAAGLPGGFYASDLTVRSNDPAAPERIVPVRLTI
                     AGSPVLRVRGEPVLLRSSLPFTAAGGSTTHTLRASPPPGGSGTLRLVAEGDFGDPTET
                     ANVALDHVHFASIGSIGLDCSAAATVAPIDATILGLLAADGTVRATFSNTGAVEPQCE
                     RNRHTVELSYRGVADRLDFGLRYVGDPRTLRLEIENAGNGRLTVSAASGAPEFVVSPG
                     ALTLAPGERRLLAVEYRPGEEGPSDSTLVISSNDPATPVIQIPLAGESRRPPRAVVAP
                     TSFAETLKAGQTVTRTLQISNEGGGTPLDFSSSIIVSRSGSSGCVPTEALVVETGAGA
                     LSRVDLLAGTSTRATDLPAIGAGLALGPGGVEAHVLQGSGGVVRVDLPTGHKEPFSSG
                     VASISGLAASLDTLYASQQATGQVVARALTGGGELLVATGLSQPGDLAVTADGRTLYV
                     IERGAGRLVRIARADAHVETVAAGLEPVGGLALDEAGGRAIVSLPGSGAIAAIDIASG
                     EVTELVDGFDLPGAIAVLGENDLLVAEVGQGRLTRV"
BASE COUNT         1303 a         3449 c         3199 g         1436 t
ORIGIN      
        1 ctgggggggg cccgacctct tcggctatgt cttcaccgac agcgacgagc cggacgggcc
       61 ggtcttcgcc tggagggaca tctccgcctc cggtcgcacc gccattctga ccggggaaga
      121 cgcgctctcg gcggcgattc cgctcgcatt cctgttcccc ttctacggcc ggaccttcac
      181 gagcgtccgg ctctccagca atggttacct gacgttcggg gacgtcaagg caccttacga
      241 gaaccagccc ctgccgagtc ccggcatttc cagcgccatg atcgcggggt actgggacaa
      301 cctgatcgtg ccgggaccac agaacgcgat ctggctctcc gagcttcaca ccttcacggt
      361 gcagtacgac ggcgtgctac gcatgagggg cggcgggccg tacacgttcc agatcgtcct
      421 cttcgactcc ggtgagatcc tgttccagta cctggagatg gtgggactcg ggtactccga
      481 gacgatcggc atccaggacg cctccgggac cgccgggctc ccgatcgcct tccattccgc
      541 gtacgtgcac gatcggctgg cggtgcggtt gctgatgcag aaggactggg tcaccgccgc
      601 cccgggctcc ggccggctgc ggtccttcga gtcggagtcg gtcgccctca ccttcgacgc
      661 ttccggtctc gcggccggaa cctacgaggg ccggctgccg atcctcacca acgatccgga
      721 tcggcggagc gtcgagatcc cgttgatgct ctccgtcgag ggcgcgcggg cgatcgccgc
      781 cgtgccggcc gcggtcgatt tcggcagtgt gttcgcggca gacggatcgc ggctcgatct
      841 cacgatcgtc aacagcggca gcctgcccgt gacggtcacc gccctcgtgc cgggcgatcc
      901 gtccgtcatc accgacttcg cgccgttcct cctcgccccg ggcgggcacc gcctgctgac
      961 acttcagtgg ctccccgccg agcccggcat cctcctggcg gacctgcgca tcgagagcga
     1021 tgccacgaac gctccgtcgc tctcggtgcc gctgaccgga atcgcgctga acatcccgcc
     1081 ccgggccgcc gcgatctggc cgccggccca ggtcgagtgc gccggaccgg acggatcgga
     1141 cgtggtgctc gacgcgtcgc cctccgggga cgacgatgca cctcccggcg aaccgaccgg
     1201 gatcgccctc tacgagtgga tcgagaaccc caggaccgcg gtcgaggagc tcctcgggac
     1261 cggcatgcgg atcgcgagga ggctcccgcc cgggacgcac cggctggcgc tgcgcgtcac
     1321 cgacagccgc ggcgccatcg acagcgtcga gagcacggtc accgtcgccg acacgacccc
     1381 gcccgagctg agcgtcaggg cgtccccgac gtttctctgg ccgccgcatc accgcatggt
     1441 cccggtgcgc ctgatcttga gcacgcggga cgcctgctcg gacgaggtga cgatgcggct
     1501 gaccgagatc tcgagcagcg agccggacga cgctcccggg aacgccgacg gccggaccac
     1561 cggggacatc gccggcgtcg aggcgggaac gcccgacacc cgcatccagc tgcgcgccga
     1621 gcgaagcgtc ccggggacgg gccgcgtcta cacgatccgc tacgtcgcga cggacgcggc
     1681 cggtcacaag acgatccggg aggcggtcgt caccgtcccg gccaacaagg tgagcgcgcg
     1741 ccgccgctga tccgcacgat ccgcgcaatg aattggctgg cgcccctttc ccgcgatggt
     1801 atattccagg cgcttctcct ctgaggggtc ctgaattgag cctccagccg cctccgacgg
     1861 cgtccgcttc tccggtgccc ctcagcctcg ggagtcagag atcccggtcg atcgccgtgg
     1921 acggcttccg ggtgaccgag gtctggttcc cgcccttcct gagactgccg ccccacacgc
     1981 acgatcgggc ctccctggcg gtgatgctcg ggggctcgtt cgacctgacc ctgagccggt
     2041 cgtcccactc ctgtccgccg gccagcgtct tcaccgaacc ggtcggcgaa cggcacggca
     2101 accggatcga cgcggccggg gcgcacgtca tcgtggtgca gccggatccg gcgcgcgcgg
     2161 acctcacgcg tccctgccgg gacctgttcg atcgcccggc ccacttccgg catgccgcga
     2221 tcaccgcgtg cgcccggcag ctcggcgccg agttgcaggc gcgcgatgcc gtgacgccgc
     2281 tggcgatcga ggggaccgtc ctgtcgatga tcgcctccgc cgcgcggctg accgagatcg
     2341 ccccgcgtcg cgccccggcg tggctggacc ggacgatcga gctgctgcac gctcgcttcc
     2401 tcgacaccct gacgaccgcc ggtgtggcgc acgaggtcgg cattcacccg gtgcacctgg
     2461 cgcgcgtctt ccgggcccgc cacggcatga cgatgggcgc gtacgtgcgc aggctgcggc
     2521 tcgactgggc ggcccgccgg ctgacccgct cccgcgaccc gctgtgcgac atcgcggtcg
     2581 cggccggctt cgccgatcag agccacttca ccagggcctt caagcgacac ctcggcgaca
     2641 cgccgcatcg cttccgccgt cgcctcgcgc cgcgtcgctg aggcgcttcc ccagcccccc
     2701 gccgttgcat ccgttccatg cgggtgctcc cgcgttcaag accccgccgc atcctccccg
     2761 tacgcttcga agcatgtcgg gtggaccgat tccatccgcg acgggaggct tcgatgcgaa
     2821 ccaagacctg gctcacggtg gtcgtcctcg tggtgtccgg cgccgcaggc gcctcggcgc
     2881 tggcggcgcg cggcggtgcg gtgcgcgatg actcgatcga gaccgccggc gcttcggccg
     2941 cggcggcggc caacgccggt cacttgctga cagcgaccgg cggataccac ttcaccatcc
     3001 cggccgactt caacggcggg atcttcggca ccctgaccga catcaagaac cgcgtcacct
     3061 tcaacgcgaa gaaggacgcc gacggcagcg tcagcggctg gtggacctac gagcaggagg
     3121 tggacggcgg ggtcttcaag ttcggcggcc cggtcacctg cctgaacatc tacgacaccc
     3181 cggtcctgac acgcacgccc gacatcccgc cgatgacaca gaaccgcgcc aagtggggcg
     3241 gccgggtgga gaagtcgaac gacgagacga ttcccgtggg ccggttcatg tggttccagt
     3301 cgatcgacaa cggcgaaggg gccaacggct attccgacgt cagcactctc gtcggcctgg
     3361 gggacgaggc ggccaacatc gccttctgca acgccgaccg cgtgccgaac tcgaacttcg
     3421 gtccgcatcg catcggaggc ggcaatatcc aggtggactg agcttcgttc cggcaccggc
     3481 gccgaccccg gccgcaaccg gccccggggt cggcggttac tcgggcctct cgggcctctc
     3541 gcgttttcgg ccccagttgt agctgtcccg cgcctggtac ccgcacttga gacaggtcgc
     3601 gtacagctcc gggtcattgg gggtccaggg gtacttcgac acctccgcat agcgcaaggc
     3661 ctggcagtct cctcggcata gcacccggac caggagtccg ggcgggatgc tcgctgcata
     3721 ggttggtcgc ttcggcatgc cgccctcccg ttgacgctcc tgaattttac tccgccaccc
     3781 ccggggattg cccgtacatt ccagagccgc aggatgatgc cgttaatgct atcctgacat
     3841 tagttttcgg ctcgctcgcg agggttcggg gaatgcgtcg tccgtcgggg ccggggtctg
     3901 ttcgacacgc ggcgattcct ctcctcgtgt cgctcgccct gatcctcgcc gccggcgccg
     3961 ccgcgccggg agctccgaag cgatcccccc ccggtccgca cggaccaaag aatccgcagt
     4021 acgtcgaagg ggagatcctg gtcaagttcc ggccccaggc gccggtcgcc aggcgcggcg
     4081 ctctgcgcag cgaggtcgcc ggggccgtca agagccgctt catgtccgga gccgagctcc
     4141 tgcgtctcgg cgccggggtc ggggtgggcc aggcgctggc gcgactcaag ggggcgcccg
     4201 aagtcgagta cgccgagccc aactacatcc tgcacgcgac caggaccccg gacgatccgt
     4261 tcttcgcgca gcagtggtcc ctcctgaaca ccggccaggc cggcggcgcg gccggatccg
     4321 acatcgacgc cacggcggcc tgggacatct cgatcggcgg cgcgtcggtc gtgatcgcga
     4381 tcgtggacac cggcatcgac ctcgaccacc cggacctcgc gcccaacctc tggaccaacc
     4441 cggacgagat ccctgggaac ctcgtcgacg acgacggcaa cgggcggatc gacgacgtcc
     4501 acggctggga cttcgtcaac cacgacgacg accccgacga cgacctcggc cacgggacgc
     4561 acgtcgccgg gacgatcgca ggggcgggcg acaacggcat cgggatcacc ggcgttgcgt
     4621 ggcgggccag cctgatgccg ctgaagttcc tcgaccggag cggcgccggc acgacctccg
     4681 acgccatcca ggcgatcgac tacgccgcga gcgagggggc gcggatcctc aacgcctcct
     4741 ggggcggcgg cggcttctcc atggcgatgc tggagtcgat ccgcgacgcc gccgcgcgcg
     4801 aggtcctgtt cgtcgcggcg gccggcaacg acgcggccga cacggacatc gcgccgttct
     4861 tccccgccgg gtacgacgct ccgaacgtcg tcgcggtcgc ggcgagcgat cgcagcgacc
     4921 gtctcgccct cttctcgagc ttcggcgaaa cgaccgtgga tctcgccgcg cccggggtgg
     4981 acatcctcag caccctgccg ggcggcacct acggtttcag cagcggcacg tcgatggcga
     5041 cgccgcacgt ctccggtgtc gcggcgctcg tcctcgggtt cgcgccggga gtcggcgctg
     5101 aatcgctgcg ccgacggctt ctcgatgagg cggagccgat cgcggcgctc gagggaaaag
     5161 tggccgtggc gggccggctg aacgcgttcg agtgcctgtc gggcgccgac gccgttcgcc
     5221 ccggtccgat cgacgacctg agcgtcgtgg agccgctgag cgatggcctg gtgctcgctt
     5281 ggaccgccac gggagatgac ggcgacgagg gggccgcggc cggttatgac ctgaggattt
     5341 cggaagatcc cttcgacctc ctggggttcg aggccgcccc ccgcttccac ctgcccggac
     5401 ctcccctccc cgccggcacg ccggagacga aggagatcgg cggactcgat ccctcgcgca
     5461 cgtactacct cggggtccgg gcgatcgacg aatgggggaa cgccgggccg cccggcttcg
     5521 cgaccgcctc gaccctccag ccgccggcca tggaggtgac gccggagacc tcgtccgccg
     5581 cgcttctcag tggccagacg gcgacgaccg tcgtcaccct ccgcaacgcc agcgcgggaa
     5641 ccctcgactg gagcatcgag cggccgatcc tccgaccgac cgcggtccgc gcgggcccgc
     5701 ggaccgagcg ctggggagga ccggacgcgt tcggctacgt gttcaccgac agcgacgaac
     5761 cggacgggcc ggtcttcgac tggaaggagg tccccgccga cggccgcagc gccctcgtcg
     5821 ccggcgacga tctgctctcg gcgccgatcc cgctcggctt cccgttcccc ttctacggcc
     5881 ggacgttcgc gagcgtccgg atcgccagca acgggttcct gagcttcacg cacgaggacg
     5941 cgccgtacga gaaccagccg ctgccgagcc ccggcgctcc cggcaacctg atcgcgccgt
     6001 tctgggacga cctgttcgtc tggggtctgc aggatgtcat ctggctcgcc gagccggaca
     6061 ccttcacggt gcaatacaac aacgtgctgc gatacggagg aggagggccg tacacgttcc
     6121 aggtggttct cttcgcctcg ggagagatcc tgttccagta cctgtcgatg acggacacgc
     6181 tcgactccgc gacgatcggc atccaggacg aatccgggac ggccgggctc ctgatcgcct
     6241 tcaacgcccc gtacgttcat gaccgcctcg ccatccggct ggcgatccag gaggaatggc
     6301 tgtccgcttc tccccgctcg ggacgattgc ggcgcggtga gtccgcgccg atcactctct
     6361 ccttcgatgc gtccggtctc ggcggcggcc tccacgaagg gtggctgccg atcgtcggca
     6421 atgatccgga ccggccgcgc ctcgagcacc cggtgctcct cacggtcgag gacgcgccgg
     6481 gcatcgccgt ggagccggcc gccgtcgatt tcgggcgggc gttccagggg tacgcatcgc
     6541 gccgcagcgt cgagattctc agcagcggca gcctgccttt gacggtccat gccgcggtcc
     6601 cggacgaccc ccgtgtcggc accgatttcg cgccgttcgt cctcgcgccg ggagagcggc
     6661 gcctgctggc gatcgactgg gtcccggcgg cgatcgggga ccttcaggcg aacctgcgca
     6721 tcgacagcgc cgcgaggaac gtcccgtccc tcaccgtgcc gctcgccgga tcggccgtcg
     6781 ctccgccggt cctgaggatc gagccggaga cgatcgacga gacggtgccg ctcggcggcg
     6841 cgtcggagcg ggtcctgtcg ctcgccaatg acggagcgac cgatctcgac tacacgatcg
     6901 aactggaccg gccgggcgag ctgtccgcgg gggcgttcga ttcctttccg ccgagcccgg
     6961 agccgctgac ctgcgtcgtg gcggacccgt cggccgggat gctctacggt caggagcggc
     7021 gcggcaggcg cttctaccgc ctccgcgtcc gtgaccagca atgggagacg ctgccgccgt
     7081 ccccgaactc ctccggagcg aactgcggcg ccgcgctcct ggccgggcgg atctacaccg
     7141 cctcgtcgga tccgccggcc gtcctcggcg tgtacgacac cgccttcgga tcgtggacga
     7201 cgctgccgag cccgctcggc gccgcgaccg ccaacatcgc ctcggacggc gatcgctggc
     7261 tctatctcgc tctggaccgg acggtgaagc gcttcgatcc tgcggtgggg ggggcgggcg
     7321 agtcccttcc ggtccccccg ttccccttcg atcggtgggg cggcctccag gtgcacgccg
     7381 gactgctgta cggccacgtc ggcaacagct ccacctggtt cggccgctac gacccgttcg
     7441 tgcgaatctg gtcgatgctg ccgttcctcc cgctcggcgg cacctccggc gcggccatcg
     7501 acccggtgag cggcgactat ttcgcggccg gccccgcggg cggcgccaac ctgtaccgct
     7561 tcgattccaa gaattccgtg tggagcacga tgccgctccc cttcccggtc gaggacggcg
     7621 gcctcgcatg gctcggcctg ccggtggggg cgatctactt cgtcgagggg gagcgcggca
     7681 cgcgcgtcgg acggctgctg ctcggcgatg cgcgggcggc ggcggtggcc tccgcatccg
     7741 gccgggtcgc tcccggcggg accggggagg tccgcctgca gttcgacgcg gcggggcttc
     7801 cggggggctt ctacgccagc gatctgacgg tccgcagcaa cgacccggcc gcgccggagc
     7861 ggatcgtccc ggtgcggctg acgatcgccg gatccccggt gctgcgggta cgcggcgagc
     7921 cggtgctcct gaggtcgagc ctgcctttca ccgcggcggg tgggagcacg acccacacgc
     7981 tgcgcgcgtc accccctccc ggcggaagcg ggactcttcg tctggtggcg gagggagact
     8041 tcggcgaccc gaccgagacc gcgaacgtcg cgctggacca cgtgcacttt gcgtcgatcg
     8101 gctccatcgg actcgactgc agcgccgcgg caactgtcgc tccgatcgat gcgacgatcc
     8161 tcggcctcct cgcggccgac ggaaccgtcc gcgcgacgtt cagcaacacc ggcgcggtcg
     8221 agccgcagtg cgagcgcaac cgccacacgg tcgagctgtc ctatcgtggc gtggcggacc
     8281 gcctcgactt cggcctgcgc tacgtgggcg atccccgcac cctgcggctg gagatcgaga
     8341 atgccggcaa cgggcggctg acggtctccg ccgcgagtgg cgcgcccgag ttcgtcgtct
     8401 ctccgggagc gctgaccctc gcgcccgggg aacgaagact cctggccgtg gagtaccggc
     8461 ccggcgagga aggcccctcc gactcgacgc tcgtgatctc cagcaacgac ccggcgacgc
     8521 cggtgatcca gattccgctc gcgggggagt cgcgccggcc gccgcgcgcc gtcgtggccc
     8581 cgacctcgtt cgcggagaca ctcaaggccg ggcagacggt gacccgaacg ctgcagatct
     8641 ccaacgaggg gggggggacc cccctcgatt tctcctcctc gatcatcgtg agccggagcg
     8701 gaagttcggg ctgcgtcccg acggaagcgc tcgtggtcga aacgggcgcc ggcgctctgt
     8761 cgcgggtcga tctgctggcc gggacatcga cccgggccac cgatctgccc gccatcgggg
     8821 ccggcctcgc cctcggcccc ggcggcgtcg aggcgcacgt tctccaggga tccggcggag
     8881 tggttcgcgt cgaccttccg accgggcaca aagagccgtt ttcgtccggg gtcgcttcca
     8941 tctccggcct cgcggcctcc ctcgacaccc tgtacgccag tcagcaagcc accggccagg
     9001 tcgtcgcccg ggcgctcacg ggcggggggg agctgctcgt cgcgaccggc ctgagccagc
     9061 ctggcgacct cgccgtgacc gcggacggac gcaccttgta cgtcatcgaa cgcggcgccg
     9121 ggagactcgt gcggatcgcg cgggccgatg cccatgtcga gactgtcgcc gccggcctcg
     9181 aaccggtcgg cgggctggcc ctcgatgaag cgggaggacg cgccatcgtc tcccttccgg
     9241 gctcgggagc gatcgccgcc atcgacatcg cgtcgggcga ggtcacggag ctggtggacg
     9301 ggttcgacct gcccggtgca atcgccgttc tcggagagaa cgacctgctc gtcgcggaag
     9361 tgggccaggg ccggctgacg cgcgtgc
//
LOCUS       DASRIQ010000397         4193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2485171, whole genome shotgun sequence.
ACCESSION   DASRIQ010000397 DASRIQ010000000
VERSION     DASRIQ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485171"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFD06_09730"
     CDS             <1..982
                     /locus_tag="VFD06_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03850.1"
                     /translation="FDPSVSYSESDPEDSSPPDRESMPIEFEEEAETPPIPPGELPAF
                     AAPPNVPTGWSVDPETGEKVHLGPIELTFDGPVVPPQQTGARRGRLALFGAAGMLAVA
                     AVVAFFVVRGNGASEPEGPAPDEIASRAAGTDAAPPGTTPEENAAPEPGAPSAPETSS
                     APGERAETPGEDAAPGADAEPAPSPTAPPIAAPPPRNDNARPREGTGATVDFGFGQRL
                     LDAGTVDRAAEAFRRAIGPIPAGRFTLQTMIACEAESVRKARAGTRPDTGLFVLPVAV
                     RGRGCFRVMWGVFETREAAAEAARSDLPQYFVSSGVSPAVVSIDRLRSPS"
     gene            987..2423
                     /locus_tag="VFD06_09735"
     CDS             987..2423
                     /locus_tag="VFD06_09735"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03851.1"
                     /translation="MSFGPRRLAALGFLLAVSSLGVVSLLWADTVHLKNGGTIACDSI
                     EERGADLVLRVGKMTIVVPRDEVERIEVGPKTGKPAPPPSGASPSQPGASGSAAAPSG
                     AGEAKGHEAEGDAKADAARLEELKRRLQAEPLARDENRRQIVALLDRMGERALSERKP
                     EEARRRFTEAAGYDPADLRARRGLGAALLMLDRTAEARTTIERALLDAPQDADLNYLL
                     GEAFERLGRADQALAALEKAYAARPTPTLRDRIATLKRQHGVDGAYRRSEAARFTLTY
                     DGARTSPQLEAEILAFLEGQYPELALLFDYSPAESIAVILYPEQDFYAATAANRDVAG
                     LYDGKVRVPSGGLRRLDPGAREVLRHELAHAFIAGKSRGACPRWLHEGLAQWVEGRRP
                     GPAGETRLAREYRAEPSRWGTLFDYDSALSFVAYLLAQHGQTALNDVLAVMGRGLTAE
                     AAFVEVLRDPLPTLRTAWGDELVRDHLP"
     gene            2509..>4193
                     /locus_tag="VFD06_09740"
     CDS             2509..>4193
                     /locus_tag="VFD06_09740"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZN03852.1"
                     /translation="MKGVLAQRCPADILSEVLRRQMSGILSLTLEPASRQLFIDAGVI
                     IRFAASNLREESLVSWLMAKGGLSEAQIREATAQKQPHELLGTVLARLGRLTPGNLTD
                     LTESHIRRVLTSTLAMTRGNWEFKEGALPFRDQLDGGVRSAEALLEWSRGLGDVEWIR
                     GRLGPVDARVHRDRRPPEGYQSIRLNSVEGFIMSLVDGAATLREICMMSPTGEDKTLK
                     ALFGLTLAGILETPPGTEAALPPPLSPRRLAEAGGPVAPYTAAAPPAPAPPAAPPAAA
                     RSARPNATVPVATVRPAPVPSRPPAAPPTSKPSAPAAAPPAPAAAAPAPPAASNAKAP
                     APAATPHAEPVASRPTAPAAAPNPAPAVPRPAAAGSGGAKPAARPGIRGNARPGGGRP
                     GQRPGSLKAPMRPRPVANPVRLAEPAKPSQGPDLDREMLTRHADLDNKDLYHALGVAS
                     SAGSDEIRRAYYALARQFHPDRFRDDQMKAKAEQVFGRITEAYATLSDQEARTRYDGE
                     LAMHHDGGAQDHAGNTTDIARENFRRGRDELDRGRFAEALAFLMHACEQDPNRS"
BASE COUNT          589 a         1597 c         1477 g          530 t
ORIGIN      
        1 gttcgacccg tcggtgtcgt actcggagtc cgatccggaa gactcgtcgc cgcccgaccg
       61 ggagtcgatg ccgatcgagt tcgaggagga ggcggagacg ccgccgatcc cccccggcga
      121 actccccgct ttcgccgccc cgccgaacgt cccgaccggc tggagcgtcg atccggagac
      181 cggagagaag gtgcacctcg ggccgatcga gctgaccttc gacggtccgg tcgtgccgcc
      241 gcagcagacc ggggcgcgcc gcggcaggct ggcgctcttc ggggccgcgg ggatgctggc
      301 tgtcgcggcg gtcgtcgcct tcttcgtcgt gcgcggcaac ggcgcctcgg aacccgaggg
      361 gcccgcgccg gacgaaatcg cctcgcgcgc cgccggaacc gacgcggccc cacccggaac
      421 gactccggaa gagaatgccg ctccagagcc cggtgcgccg tccgccccgg aaacctcgtc
      481 ggcgcccggc gaacgtgcgg agactcccgg cgaagacgcg gcaccagggg cggatgccga
      541 gccggcgccg tcaccgaccg ccccgccgat cgcggcacca ccgccgcgga acgacaacgc
      601 gcggccgcgg gaggggaccg gagcgaccgt cgatttcggg ttcggccagc ggctgctcga
      661 cgccggcacc gtcgatcgcg cggcggaggc gttccgccgc gcgatcgggc cgatcccggc
      721 cggccgcttc accctgcaga cgatgatcgc ctgcgaggcg gagagcgtgc gcaaggcgcg
      781 cgccggcacc cgtccggaca ccgggttgtt cgtcctgccg gtcgcggtgc gcggccgcgg
      841 ctgcttccgg gtgatgtggg gggtgttcga gacgcgcgag gcggcggcgg aggcggcccg
      901 ctccgatctc ccccagtact tcgtctcctc cggcgtatcg ccggcggtcg tgtcgatcga
      961 tcggctgcgc tccccgtcct gacctgatgt ccttcgggcc ccgtcgtctc gcggcgctcg
     1021 gatttctgct cgccgtctcg tcgctcggcg tcgtgtcgct cctttgggcc gacaccgttc
     1081 acctgaaaaa cggcggcacc atcgcctgcg acagcatcga ggaacgcgga gcggatctcg
     1141 tcctgcgcgt cgggaagatg accatcgtcg tcccgcgcga cgaggtggag cggatcgagg
     1201 tggggccgaa gaccggcaag cccgctcccc ctccgtcggg cgcgtccccg tcccaaccgg
     1261 gagcgtccgg gtccgccgcg gcgccctcgg gggccggcga agccaaagga cacgaggccg
     1321 aaggggatgc caaggcggat gccgcccggc tcgaggagct caagcggcgg ctccaggctg
     1381 agcccctggc gcgggacgag aaccggcgtc agatcgtcgc gctgctcgat cgcatggggg
     1441 agcgggccct ctccgagcgc aagccggagg aggcgcgccg gcgcttcacc gaggcggccg
     1501 gctacgatcc cgccgatctg cgggcgcgcc gggggctcgg cgccgcgctt ctgatgctgg
     1561 accgcaccgc ggaggcccgc acgacgatcg agcgggctct cctcgacgcg ccgcaggacg
     1621 ccgatctcaa ctacctgctc ggcgaggcgt tcgagcggct ggggcgcgcg gaccaggcgc
     1681 tggcggcgct cgagaaggcc tacgcggcgc gtccgacgcc gaccctcagg gaccgcatcg
     1741 cgacgctcaa gcgccagcac ggtgtcgacg gcgcgtaccg ccgctcggaa gccgcccgct
     1801 tcaccctcac ctacgacggc gcgcgcacgt ccccgcagct cgaggcggag atcctcgcgt
     1861 tcctcgaagg ccagtacccg gagctggcgc tcctcttcga ttactcgccg gccgagtcga
     1921 tcgcggtgat tctctatccc gagcaggatt tctacgccgc cacggcggcc aaccgggacg
     1981 tggcgggcct ctacgacggc aaggtgcgcg tgccgtcggg tgggctcagg cgcctcgacc
     2041 cgggcgctcg cgaggtgctg cggcacgagt tggcgcacgc cttcatcgcc ggcaagagcc
     2101 gcggcgcctg tccccgctgg ctgcacgagg ggctcgccca gtgggtcgag gggcgccggc
     2161 cggggccggc gggagagacc cgcctcgccc gcgagtaccg cgccgagccg tcccgctggg
     2221 gaacgctgtt cgattacgat tccgcactct cgttcgtcgc gtacctgctc gcccagcacg
     2281 gacagacggc gctgaacgat gtcttggccg tcatggggcg cggcctgacc gccgaggccg
     2341 cgttcgtcga ggtgctccgc gatcccctcc cgaccctccg gaccgcctgg ggggacgagc
     2401 tggtccggga tcacttgcca taggccacct cgaaacccaa gttatctccg ccccttgccc
     2461 gagcgcaggg caaggagggg tccgtccctg cgctggaggt cgccccggat gaagggagtc
     2521 ctggcccaga gatgtcccgc cgacatcctg agcgaggtcc tgcggcggca gatgtccggc
     2581 atcctgagcc tcaccctgga gccggcgagc cggcagctgt tcatcgacgc cggcgtcatc
     2641 atccgcttcg ccgccagcaa cctccgggag gagagcctcg tctcctggct catggcgaaa
     2701 ggcggtctga gcgaggcgca gatccgcgag gcgacggcgc agaagcagcc gcacgagctt
     2761 ctcggaacgg tcctggcgcg gctcggacgc ctcacccccg ggaacctcac cgacctcacc
     2821 gagtcgcaca ttcgccgggt cctgacctcg accctggcga tgacccgggg aaactgggag
     2881 ttcaaggagg gggcgctgcc gttccgcgac cagctcgacg gcggcgtgcg cagcgccgaa
     2941 gcgctgctcg agtggagccg cggcctcggc gacgtggagt ggatccgcgg ccgcctggga
     3001 cccgtcgacg cacgggtgca ccgcgatcgg cgtccgccgg aagggtacca gtcgatccgt
     3061 ctcaattcgg tcgagggatt catcatgtcc ctcgtcgacg gcgcggccac cctccgcgag
     3121 atctgcatga tgagcccgac cggggaggac aagacgctca aggccctctt cggactgacg
     3181 ctcgccggga ttctcgagac gccgcccggg accgaagcgg ccctgccccc tccactttcc
     3241 ccgcgccgcc tcgccgaagc gggcggtccg gtggcgccgt acacggccgc ggcgcctccc
     3301 gcgccggctc cgccggccgc gccgcccgcg gcggccaggt ccgcgaggcc gaacgccacg
     3361 gtccccgtcg cgacggtccg cccggccccg gtcccatcgc gcccaccggc tgcaccgccg
     3421 acatcgaagc catccgcgcc ggccgcagcc cctcccgcgc cggcggcggc cgcgcccgcg
     3481 ccgcccgccg cttcgaacgc gaaagcgccg gcaccggcgg cgacaccgca cgctgaaccc
     3541 gttgcgtccc gtccgaccgc ccccgccgcg gccccgaacc ccgcgccggc cgtgccgcgt
     3601 ccggccgccg ccggcagcgg cggagcgaag ccggccgctc gtccggggat ccggggaaac
     3661 gcgcgacccg gaggcggtcg tcccggccaa cgccccggaa gcctgaaggc tcccatgcgc
     3721 ccgcgaccgg tcgcgaaccc ggtccgcctt gcggagccag cgaagccttc ccaagggccc
     3781 gacctcgatc gcgaaatgct gacgcgtcac gccgatctgg ataacaagga cctgtaccac
     3841 gccctcggcg tcgcctcgag cgccggaagc gacgagatcc gccgcgccta ctacgcgctg
     3901 gcccgccagt tccacccgga caggttccgc gacgaccaga tgaaggcgaa ggcggagcag
     3961 gtcttcggtc gcatcaccga ggcttatgcg acgctgtcgg atcaggaagc ccgcacccgc
     4021 tacgacggtg agctcgccat gcaccacgac ggcggcgcgc aggaccacgc cgggaacacg
     4081 accgacatcg cgcgcgagaa cttccgccgc ggccgcgacg agctggaccg cggccgcttc
     4141 gcggaggcgc tcgccttcct gatgcacgcc tgcgagcagg acccgaaccg atc
//
LOCUS       DASRIQ010000398         7183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2485506, whole genome shotgun sequence.
ACCESSION   DASRIQ010000398 DASRIQ010000000
VERSION     DASRIQ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485506"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFD06_09745"
     CDS             <1..851
                     /locus_tag="VFD06_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03853.1"
                     /translation="FQDSLAARWRGAWVVTNAETYSDCAGVYTGNRINGRLVSGKAAL
                     RFKSGELAKVDSVDLKRSRLDLKLSFPEPVLRAYQDGPFSLYDEAQCRVELQIELPRE
                     MVKSQDVMGVERLLRPVVERHATEDAARASGRFNERECDPYPADYSMTVAKHAAWRAE
                     QTNLAVRTNIDHLVDEASRITERIGEDPDYISGFVQGVEAGRAPHPVACPDLMALATG
                     TPPGYALPGTGNVAQAARRGQTAVISTEAQARRQRGYQDGLRLSLGLDAIRRLPACFV
                     PVPDAD"
     gene            complement(856..1491)
                     /locus_tag="VFD06_09750"
     CDS             complement(856..1491)
                     /locus_tag="VFD06_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03854.1"
                     /translation="MPRSIPSRPTFWRPAPLALLTGLALFAALGGFATPAQAGDVNLN
                     FYPVSGRSLDDDLWSPVEDQWGFGGTVDFGEKGNPLHMALGFHVGVGAEDLDSPVTDD
                     VVGVVTELSVGIAGAWQSQGRLRGYVAGGFSFVGAAIEVDTIFGDVDDDDDSLGGWIE
                     AGMAWRLGQHFSLGFGARTLIGTDITLFGVEGDADYFQFGPLLAWSWPARQ"
     gene            1529..3085
                     /locus_tag="VFD06_09755"
     CDS             1529..3085
                     /locus_tag="VFD06_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HZN03855.1"
                     /translation="MKALQQSLYDLIVRTSTDLPPDVRRAMARALGDEDQANRSGQAL
                     QIIATNIDAAADCEGPICQDTGWPTFEVTTPVGVSQIQIEKAIRAAIVEATKKGKLRP
                     NSVDSLTGKNSGDNLGAGTPAIHLHQWENDAEIEVRLLLKGGGCENKNIQYSLPAELP
                     HLGRADRDLDGVRKCLLHAVWQAQGQGCSAGALGAAIGGDRATGYAHAKEQLFRTLDD
                     VNPNADLRRLEEYVMEKSNLLGIGTMGFGGGVTLIGCKVGAVHRLPASFFVSVAYDCW
                     AFRRLGVILDAKTGALKRWLYRDDEDSKPIAAASATPGGAGLFGSAGAVALKPPLSEE
                     SIRRLKVGDVVTISGLIYTGRDIVHHHLMSHAPPMSLKDQVLYHCGPVVMKENGSWRV
                     TAAGPTTSIREEPYEADVIGRYGVRVVMGKGGMGAKTLAALKEHGAVYLNAIGGAAQY
                     YARCIEKVEGVDLLEFGTPEAMWHLRVKDFPAVVTMDASGRSLHADVEGASAAILKTF
                     APPVFTSQPA"
     gene            3094..3939
                     /locus_tag="VFD06_09760"
     CDS             3094..3939
                     /locus_tag="VFD06_09760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN03856.1"
                     /translation="MAPASRKPEPAGRIRFAGTRVPSPGPRIDLSGQAAIVTGGSRGI
                     GRATVEALAQAGASVVFSFHRNRAAAEEVVERAGADRVVAVRADVSQPRDADRLVEAA
                     RRRHHRVDVLVNNAGIWDPPGGTPFENLSERAWRRTMAINLDGAHLVTRRVIPLMKRR
                     RSGRIVFVSSTAGQRGEERHGEYAATKGALISLTKSLAVELGPHGILVNAVAPWWVDT
                     DMSHAALVRLRRGRELKRGEWSPLDRVAAPEEIAGPILFLCSHLATYVTGEILNVNGG
                     TILCG"
     gene            3975..5153
                     /gene="hisC"
                     /locus_tag="VFD06_09765"
     CDS             3975..5153
                     /gene="hisC"
                     /locus_tag="VFD06_09765"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZN03857.1"
                     /translation="MSDAELTRRRFAGAIGAALGGALLGSRSAVAAPPSSRTPRSPTA
                     PAPEVSRLAPGPVRLDSNENPYGPPKVALDAMGRSLARGARYPDALEDELTAVLARLH
                     HVEPDQVILGCGSGEVLKMADEAFLMPAEKVVVAEPTFEAVTAFAEVVRAEQARIPLD
                     KKFAHDLDAMAAACDHRTGIVYLCNPNNPTGTIVPAGLVGAFLRNVPKDTVVLVDEAY
                     HHFVEDPAYGSADQWIAEYPNLVVVRTFSKIYGLAGMRLGYGLGSKQRIAAMGRRKCW
                     SNTNAAVLEAALALLQTTGLVTEQRKLINDTRRWLCDELRKDGRTFIPSHTNFVMIHL
                     GKDVGPVIQAFRDKEIRTGRRFPSMPDWLRISIGTRAETATFLKALRAIVPAKATAAA
                     "
     gene            5277..>7183
                     /locus_tag="VFD06_09770"
     CDS             5277..>7183
                     /locus_tag="VFD06_09770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HZN03858.1"
                     /translation="MRPTSLPALLRFAALTVALLGAPPLATIPSGVVPAAGTPAPVKA
                     GRPESKPGEVLVKFKPGTTKILKDKTRDEIAARARRRFRHGAEHWILGGGHSTEEAIE
                     RLRRNPRVEYAEPNYIVRADRAPDDPIYPQLWGLRNTGQNGGIAGSDIRAESAWQVTT
                     GSPAVVVGVLDSGIDYRHPDLAPNIYRNPGEVEGNGIDDDGNGFVDDVRGWDFANDDN
                     DPFDDIGHGTHVAGTIGAVGNNGLGMAGVAWHVALVPIKILDADGVGTNADAIAALEY
                     ATGIGALITNNSWGGGAASNALLQAILEAGAAGGLFVAAAGNGANDSDLDRRYPSGYL
                     ASNIVSVAATNRSDQLASFSNYGATSVDLAAPGVEILSTLPGGRYELRTGTSMAAPHV
                     AGVAALLRSVEPAIDVAALKDRILAMTDPLPSLQGKMVTGGRLNAFLSVAAPESVPPG
                     AITDLAVVSRNSFTVTLGFTATGDDGPLGTASAYDIRSSLFPIDGASFGAATPAPGAA
                     VPGAAGSAERVEVRGLAAATTYHFALKAIDEWGRAGPISNLASGTTLGPPAVSVQPAS
                     VEADLPVGGVATRTVTLRNDGAGDLTFDIVVRKPGAGVAARPPAIELPGRPAGVALAG
                     EGDRRRFTPAGG"
BASE COUNT         1103 a         2582 c         2524 g          974 t
ORIGIN      
        1 aattccagga ctctctcgcg gcgcgctggc gcggcgcgtg ggtcgtgacg aacgccgaga
       61 cctactccga ctgcgccggt gtctacaccg gcaaccggat caacggccgg ctggtgagcg
      121 gcaaggcggc gctgcgcttc aagtccggag agctggcgaa ggtcgacagc gtcgatctca
      181 agcgatcgcg cctcgacttg aagctgagct tccccgagcc ggtgctacgc gcttaccagg
      241 acgggccgtt ctccctgtac gacgaggcgc agtgccgggt cgagctgcag atcgagctgc
      301 cgcgcgagat ggtgaagtcg caggacgtca tgggggtcga gaggctgctc cgccctgtcg
      361 tggagcggca cgcgaccgag gacgcggcgc gcgcctccgg caggttcaac gagcgggagt
      421 gcgacccgta cccggccgat tattcgatga ccgtcgcgaa gcacgccgcc tggcgggccg
      481 agcagaccaa cctcgccgtc cggacgaaca tcgaccacct ggtggacgag gcgtcgcgga
      541 tcaccgagcg gatcggcgag gaccccgact acatctccgg gttcgtgcag ggggtggagg
      601 caggccgcgc gccgcatccg gtcgcctgcc cggatctcat ggcgctcgct acggggacgc
      661 ctccgggcta tgcgctgcca ggcacaggaa atgtcgccca ggccgcgcgc cgtggccaga
      721 ccgcggtgat ctccaccgaa gcgcaggcgc ggcggcagcg cggctaccag gatggcctgc
      781 gcctctccct cggcctcgac gccatccggc gcctgccggc ctgcttcgtc ccggtcccgg
      841 acgccgacta ggctcctact ggcgcgccgg ccaggaccag gcgaggagcg ggccgaactg
      901 gaagtagtcg gcgtccccct cgacgccgaa cagggtgatg tcggtgccga tgagggtccg
      961 ggcgccgaag ccgaggctga agtgctggcc gaggcgccag gccatgccgg cctcgatcca
     1021 gccgccgagg ctgtcgtcat cgtcgtccac gtctccgaag atggtgtcca cctcgatggc
     1081 ggcgccgacg aaggagaacc cacccgcgac gtagccgcgc aggcggccct gcgactgcca
     1141 tgccccggcg atgccgaccg acagctcggt caccacgccc accacgtcgt ccgtcaccgg
     1201 gctgtcgagg tcctccgctc cgacccccac gtggaagccg agggccatgt gcagcgggtt
     1261 ccccttctcg ccgaagtcca cggtgccgcc gaaaccccac tggtcctcga ccggcgacca
     1321 gaggtcatcg tcgagcgaac ggccggagac cgggtagaag ttcaggttga cgtcgccggc
     1381 ctgcgccggg gtcgcgaacc ctcccagggc cgcaaacagg gcgaggccgg tgaggagcgc
     1441 aagaggggcg ggacgccaga aggtcggtcg tgacgggatc gaacgcggca tcgggtcacc
     1501 tccgggcggc gttgtatcat gccgggctgt gaaagcgctc cagcagagtc tctacgatct
     1561 gatcgtccgc acctcgaccg acctgccgcc cgacgtgcgg cgcgccatgg cgcgcgccct
     1621 cggcgacgag gaccaggcca accgcagcgg ccaggcgctg cagatcatcg cgaccaacat
     1681 cgacgcggcg gccgactgcg aggggccgat ctgccaggac accggctggc cgacgttcga
     1741 ggttaccacc ccggtcggcg tcagccagat tcaaatcgag aaggcgatcc gcgccgccat
     1801 cgtcgaggcg acgaagaagg ggaagctgcg ccccaactcg gtcgactccc tcaccggaaa
     1861 gaacagcggc gacaacctcg gcgccggcac gccggcgatc cacctgcacc agtgggagaa
     1921 cgacgctgag atcgaggtgc gcctgctcct caaggggggc gggtgcgaga acaagaacat
     1981 ccagtactcc ctgccggccg agctgccgca cctcgggcgc gccgaccgcg acctcgacgg
     2041 ggtgcgcaag tgcctgctgc acgccgtctg gcaggcgcag gggcagggat gcagcgccgg
     2101 ggccctcggc gccgccatcg gcggtgaccg cgccaccggc tacgcccacg ccaaggagca
     2161 gctcttccgc accctcgacg acgtcaaccc gaacgccgac ctgcgccggc tcgaggagta
     2221 cgtcatggag aagtcgaacc tccttggcat cggcacgatg gggttcggcg gcggcgtgac
     2281 gctcatcggc tgcaaggtcg gcgcggtcca ccggctgccg gcgtcgttct tcgtctcggt
     2341 ggcgtacgac tgctgggcgt tccgccggct cggggtgatc ctcgacgcga agaccggcgc
     2401 gttgaagcgc tggctgtacc gcgacgacga ggactcgaag ccgatcgccg ccgcctccgc
     2461 gacgccgggc ggggccggcc tcttcggatc ggccggcgcc gtggcgctga agccgccgtt
     2521 gagcgaggag tcgatccgcc gcctcaaggt gggcgacgtc gtgacgatca gcggcctcat
     2581 ctacaccggc cgcgacatcg tgcatcacca tctgatgtcg cacgcgccgc cgatgagcct
     2641 caaggaccag gtgctgtacc actgcggccc ggtcgtgatg aaggagaacg gctcatggcg
     2701 cgtcaccgcc gccggcccga ccacctccat ccgggaggag ccctatgagg cggacgtgat
     2761 cggccgctac ggcgtgcgcg tggtgatggg gaaggggggg atgggggcga agacgctcgc
     2821 cgccctcaag gagcacggcg ccgtctatct caacgccatc ggcggcgcgg cgcagtacta
     2881 cgcccgctgc atcgagaagg tggaaggggt cgatctcctc gagttcggca ccccggaggc
     2941 gatgtggcac ctgcgggtga aggacttccc ggcggtcgtc acgatggacg cctccggccg
     3001 ttcgctgcac gccgacgtcg aaggggcctc ggccgccatc ctcaagacct tcgccccgcc
     3061 ggtgttcacg tcccagcccg cctgagagcc cgaatggcgc ccgcgtcgag gaagccggag
     3121 ccggcgggac ggatccggtt cgcggggacg cgcgtgccgt cgcccgggcc gcgcatcgat
     3181 ctgtcggggc aggcggccat cgtcaccggc ggatcgcgcg gcatcggccg ggccaccgtc
     3241 gaggcgctgg cgcaggcggg ggcctcggtc gtcttctcct tccatcgcaa ccgcgcggcc
     3301 gccgaagagg tcgtcgagcg tgccggggcc gaccgcgtcg tcgccgtgcg ggccgacgtg
     3361 tcgcaacccc gcgacgcgga tcgcctcgtc gaggcggcac gccggcggca ccaccgggtg
     3421 gacgtcctgg tgaacaacgc cggcatctgg gatcctcccg gcggcacgcc gttcgaaaac
     3481 ctgagcgagc gggcctggcg ccggacgatg gcgatcaacc tcgacggcgc ccacctggtc
     3541 acgcgccgcg tcatccccct gatgaagcgg cggcggtccg ggcggatcgt cttcgtctcc
     3601 tcgacggccg ggcagcgggg agaggagcgg cacggcgagt acgcggccac caagggggcg
     3661 ctcatctcgc tgaccaagtc gctggcggtc gagctggggc cgcacggcat cctggtgaac
     3721 gccgtggccc cgtggtgggt ggacaccgac atgagccacg ccgcgctcgt caggctgcgg
     3781 cgcggccggg agctgaagcg cggcgagtgg tcgccgctcg accgggtcgc ggcgccggag
     3841 gagatcgccg gcccgatcct gttcctctgc tcccacctcg cgacctatgt caccggcgag
     3901 atcctcaacg tcaacggcgg cacgatcctc tgtggatgag gagggatccc ggcccgaccg
     3961 ggaaggagac cgacatgtcc gatgctgaat tgacccgccg gagattcgcc ggtgcgatcg
     4021 gggccgccct cggcggggcg ctcctcgggt cgcgttccgc cgtggccgcc ccaccctcct
     4081 ctcggacgcc acgcagcccg accgctccgg cgcccgaggt ctcgcggctc gccccgggac
     4141 cggtgcggct cgactcgaat gagaacccgt acgggccgcc gaaggtcgcg ctcgacgcga
     4201 tggggcgctc gctcgcccgc ggtgcgcgct acccggacgc gctcgaagac gagctgaccg
     4261 ccgtcctggc gcgtctgcac cacgtcgagc cggaccaggt catcctcggc tgcgggtcgg
     4321 gggaagttct caagatggcc gacgaggcgt tcctcatgcc ggccgagaag gtggtggtcg
     4381 cggagccgac cttcgaggcg gtgaccgctt ttgcggaggt ggtgcgggcc gagcaggcga
     4441 gaatccctct cgacaagaag ttcgcgcacg acctcgacgc gatggccgcc gcctgcgacc
     4501 accggaccgg aatcgtgtac ctgtgcaacc ccaacaatcc caccgggacg atcgttcccg
     4561 ccgggctcgt cggagcgttc ctccggaacg tgccgaagga cacggtggtg ctcgtggacg
     4621 aggcgtacca ccacttcgtc gaggatccgg cctacggcag cgccgaccag tggattgcgg
     4681 agtacccgaa cctcgtggtg gtccggacct tctcgaagat ctacggcctc gccggcatgc
     4741 gcctcggcta tggactcggc tcgaagcagc gcatcgccgc catgggacgc cgcaagtgct
     4801 ggagcaacac caacgccgcg gtcctcgagg cggccctggc gctcctccag accaccgggc
     4861 tcgtcaccga gcagcgcaag ctcatcaacg acacgcgccg ctggctgtgc gacgagctcc
     4921 ggaaggacgg gcggaccttc atcccgtccc acaccaactt cgtgatgatc cacctgggca
     4981 aggacgtcgg cccggtcatc caggcgttcc gcgacaagga gatccggacg ggacggcgct
     5041 tcccctcgat gccggactgg ctgcggatct ccatcggcac ccgggccgag accgcgacgt
     5101 tcctcaaggc gctccgggcc atcgtccccg cgaaggcgac cgcagccgcc tgaaggcgcc
     5161 gcatggcccc gcccacgggt cgtggtacaa ggtgtcctat cgctctgccg gtcgacacaa
     5221 tccatggcgg gagggagatc ggcgtgcctc gcaggcccgg aaccgatgtc cgagggatgc
     5281 gtccgacgtc cctcccggct ctcctgcgct tcgcggccct gacggtcgcc ctcctcggcg
     5341 ccccgcctct cgcgaccatc ccttccggcg tcgtgccggc cgctggaacg cccgcgccgg
     5401 tgaaggccgg tcgccccgaa tcgaaacccg gggaggtgct ggtcaagttc aagccgggga
     5461 cgacgaagat cctgaaggac aagacccggg acgagatcgc cgcccgcgcg cggcgccggt
     5521 tccggcacgg cgccgagcac tggatcctcg gcggcggcca ctcgaccgaa gaggcgatcg
     5581 agcgcctgcg gcgcaatccc agggtcgagt acgccgagcc gaactacatc gtgcgcgccg
     5641 accgcgcccc cgacgatccg atctatccgc agctctgggg cctgcgcaac accgggcaga
     5701 acggggggat cgccggctcc gacatccgcg cggaatcggc gtggcaggtc accaccggga
     5761 gtcccgccgt cgtggtcggc gtactcgaca gtggcatcga ctaccgtcac cccgatctgg
     5821 cgccgaacat ctaccgcaat ccgggtgaag tcgaggggaa tggaatcgac gacgatggca
     5881 acgggttcgt ggacgatgtc cgcggctggg acttcgccaa cgacgacaac gatcccttcg
     5941 acgacatcgg ccacggaacg cacgtggccg ggaccatcgg ggcggtgggg aacaacggcc
     6001 tggggatggc cggggtcgcc tggcatgtcg cgctcgtgcc gatcaagatc ctcgatgccg
     6061 acggcgtggg tacgaacgcc gatgccatcg ccgccctcga atacgcgacc gggatcggcg
     6121 ccctcatcac caacaactcg tgggggggtg gggccgcctc gaatgcgctg ctgcaggcca
     6181 tcctggaggc cggcgcggcc ggcggtctct tcgtcgcggc cgcgggcaac ggtgcgaacg
     6241 acagcgacct cgatcggcgc tatccgtcgg ggtacttggc gtcgaacatc gtgtcggtcg
     6301 ccgccaccaa ccgcagcgac cagctcgcct ccttctcgaa ctacggcgcc acttcggtcg
     6361 acctggccgc ccccggggtc gaaatcctga gcacgctccc cggcggacgc tacgaactca
     6421 ggaccgggac ctccatggcc gcgccgcacg tcgccggcgt cgccgcactg ctgcggagcg
     6481 tcgagccggc gatcgacgtg gcggcgctca aggaccgcat ccttgcgatg accgatccgc
     6541 ttccctccct gcagggcaag atggtgaccg gcggccgtct gaacgcgttc ctgagcgtcg
     6601 ccgctcccga gtcggttccg ccgggcgcga tcaccgacct cgcggtggtg tcccgcaaca
     6661 gcttcacggt gacgctcggc ttcaccgcca cgggggacga cgggccgttg gggaccgcct
     6721 ccgcttacga catccgctcc tccctcttcc ccatcgacgg cgcgagcttc ggcgcggcga
     6781 cgccggcccc gggcgccgcc gtcccgggcg ccgccggcag cgcggagcgc gtcgaggtcc
     6841 gggggctggc ggcggcgacg acgtatcact tcgccctcaa ggcgatcgac gagtggggac
     6901 gggccggtcc gatctcgaac ctcgcgagcg gcaccaccct cggcccgccg gctgtctccg
     6961 tgcagccggc gagcgtcgag gccgacctgc cggtcggcgg cgtcgcgacg cgaaccgtca
     7021 cccttcgcaa cgacggcgcc ggcgacctga cgttcgacat cgtcgtccgg aagcccgggg
     7081 ccggcgtggc cgcccggccc ccggcgatcg agctgccagg gcgcccggcc ggtgtcgctc
     7141 tcgccggcga aggggaccgc cggcgtttca ctcccgccgg cgg
//
LOCUS       DASRIQ010000399         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1979881, whole genome shotgun sequence.
ACCESSION   DASRIQ010000399 DASRIQ010000000
VERSION     DASRIQ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979881"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFD06_09775"
     CDS             complement(<1..354)
                     /locus_tag="VFD06_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006882282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03859.1"
                     /translation="MSSRLLDMREAADSVWRAIYEDMLRLSLARQPRWFQPLLRKHRR
                     LQTMTGYDHWSRAWEYPWAVFAADLDGAPKRILDVGGGGSPFAPWLAARGHDCHVVDP
                     SLDQGRFFAWDPGRSA"
     gene            442..2148
                     /locus_tag="VFD06_09780"
     CDS             442..2148
                     /locus_tag="VFD06_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03860.1"
                     /translation="MSPSRRVLFMGVGAVFTAALVLSFPAGRAGAQGHGEHGPAAAGA
                     ATSPKLMTGLSDHTHPIATTNPEAQKYFDMGLKLSFAFNKEEAERAFRKAAELDPKAA
                     MPHWGVAYALGPDINTPRDPARDKGAAEAIGKARLLAPGAPEAERAYIEAMSVRYSTD
                     AKADLRKLDEAYRDAMKRLVAKYPDDLDAATIYAESIMDLSPWKFWGPDGKPGADTPE
                     LVAVLESVLRRDPEHIGANHLYIHAVEASPHPEKALASARRLETAAPAAGHLVHMPGH
                     IYLRTGDFAQTVAMNEKAVAADDAFFRDFGKSAMYPIMYATHNSHFVAYGAMMAGMKE
                     KAVEAADRVVAEMRAATPPGALDPTMGPFLEMYGAVEITSRWRFGMWDEILKLKTPDA
                     RFVIDTALWRAARGAAYAAKKSRALALEEQKAFRAQREKIPPTWAHAFSPAQDFLAVA
                     GFIMQARLLEGVGDHDGAIASWNAAVQAADNLPYNEPPEWFYPVRESHGGALLRSGRS
                     AEAEKVFRADLEKNPRNARSLFGLWKSFEAQKKTADAALVKRQFETAWKDATVTLDVK
                     DL"
     gene            2162..3304
                     /locus_tag="VFD06_09785"
     CDS             2162..3304
                     /locus_tag="VFD06_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014245908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN03861.1"
                     /translation="MDFSLSEEQQQLQALAREFAEKEIVPKAAHHDETGEFPRDICKK
                     AWDLGLMNTHIPAEYGGAGLGVVDGCILAEEIARGCTGIGTAMEANTLAEGPIIVGAS
                     DEQKRRILTPMTEEFRLAAYCVTEPQAGSDVAGMKTTARKTGDDYVLKGQKMWITNGS
                     VADWYFVVAYTDEAQKHRGMSAFYVPRDAPGIEVGKKEKNMGQRASDTRAITFNDVVV
                     PKKDMIGAEGKGWLLAMAAFDHSRPIVAAAAVGLARAAMEHAARYAKERTAFGVPIYK
                     HQTIAFMVAEMAMKIEAARLLVWQSAWKIDRGERNTREAAMAKAFAADSAMQVATDAV
                     QVFGGYGFSKEYPVEKLMRDAKIFQIYEGTSQIQRLIIAREIFERG"
     gene            3404..>3721
                     /locus_tag="VFD06_09790"
     CDS             3404..>3721
                     /locus_tag="VFD06_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03862.1"
                     /translation="MGRKRRPGAWIAVIAVLPLGLVAAGAPGEVAGGKGDKRAGVTGA
                     PAVERRGTPVLGPRGRRETVTDIMDRERFRKSEPGRDDVEREEARLRPVPPRTNPDAP
                     GVSA"
BASE COUNT          643 a         1254 c         1330 g          494 t
ORIGIN      
        1 ggcgctgcgg cccgggtccc acgcgaagaa gcgcccctgg tcgagcgacg ggtcgacgac
       61 gtggcagtcg tggccgcggg ccgcgagcca cggcgcgaac gggctgccgc ctccgccgac
      121 gtcgaggatc cgcttcggcg cgccgtcgag gtcggcggcg aacaccgccc acggatactc
      181 ccaggcgcgc gaccagtggt cgtagccggt catcgtctgc agccgccggt gcttccggag
      241 caggggctgg aaccagcgcg gctggcgcgc gagcgacagt cgaagcatgt cctcgtaaat
      301 ggcgcgccag acggaatcgg ccgcctcccg catgtccagc agtctggagg acatggcggg
      361 atcttacacg tgctcgcgtt gacgctcctg ccgacgatct ctagactgcc tccctcattg
      421 aggggagcga cgggtatgca cgtgagtcca tcgcgacggg tgctgttcat gggggtcggg
      481 gccgtcttca ccgcggccct cgtgctttcg tttcctgccg ggagggcggg ggcgcagggg
      541 catggagagc atggcccggc cgccgcgggc gcggcgacgt cgccgaagct gatgacgggg
      601 ctgtcggatc acacgcatcc gatcgccacg accaaccccg aggcacagaa gtacttcgac
      661 atgggcctca agctttcctt cgccttcaac aaggaggagg cggagcgggc cttccgcaag
      721 gcggcggagc tcgacccgaa ggcggcgatg ccgcactggg gtgtcgccta cgcgctgggg
      781 ccggacatca acacaccgcg cgatccggcg cgggacaaag gggcggcgga ggcgatcggg
      841 aaggcgcgcc tcctcgcgcc cggtgcaccc gaagcggagc gggcctacat cgaggcgatg
      901 tcggtgcgct attcgaccga cgcgaaggcg gacctgcgca agctcgacga ggcgtatcgc
      961 gacgccatga agcggctcgt ggcgaaatac cccgacgatc tcgacgccgc gacgatctac
     1021 gccgagagca tcatggacct cagcccgtgg aagttctggg ggccggacgg gaagccgggg
     1081 gccgataccc ccgagctggt cgcggtgctc gaatcggtgc tgcggcgcga tccggagcat
     1141 atcggcgcga accatctcta catccacgcg gtcgaggcgt ccccgcatcc cgagaaggcg
     1201 ctcgccagcg cccggcgtct cgagaccgcc gcccccgccg ccggccacct cgtgcacatg
     1261 ccggggcaca tctacctgcg cacgggggat ttcgcccaga cggtcgccat gaacgagaag
     1321 gcggtggcgg ccgacgacgc gttcttcagg gacttcggga agagcgcgat gtacccgatc
     1381 atgtacgcca cgcacaactc gcacttcgtg gcgtacgggg cgatgatggc cggcatgaag
     1441 gagaaggcgg tcgaggcggc cgaccgggtc gtggccgaga tgcgcgccgc gacgccgccc
     1501 ggggccctcg atccgacgat ggggccgttc ctcgagatgt acggcgccgt ggagatcacc
     1561 tcgcgctggc gcttcgggat gtgggacgag atcctgaagc tgaagacgcc cgacgcccgc
     1621 ttcgtcatcg acacggcgct gtggcgcgcc gctcgtggcg cggcgtacgc ggcgaagaag
     1681 agccgcgcgc tggcgctcga ggagcagaag gcgtttcgcg cccagcgcga gaaaattccc
     1741 cccacctggg cgcacgcctt cagcccggcg caggacttcc tggcggtggc cggcttcatc
     1801 atgcaggcgc gcctcctcga aggggtcggc gatcacgacg gggcgatcgc ctcgtggaac
     1861 gcggccgtcc aggcggccga caacctgccg tacaacgagc cgccggaatg gttctacccg
     1921 gtgcgcgagt cgcacggcgg ggcgctcctg cgctccggac gctccgccga ggcggagaaa
     1981 gtgttccgcg ccgacctcga gaagaacccg cgcaacgccc gctcgctctt cggcctgtgg
     2041 aagagcttcg aggcgcagaa gaagaccgcc gacgccgccc tcgtgaagcg gcagttcgag
     2101 acggcctgga aggacgcgac cgtcacgctc gacgtcaagg atctgtgagg gagcagcgat
     2161 gatcgatttc agcctgagcg aggagcagca gcagctccag gccctcgccc gcgagttcgc
     2221 cgagaaggag atcgtcccga aggcggcgca ccacgacgag accggcgagt tcccgcgcga
     2281 catctgcaaa aaggcctggg atctcggcct gatgaacacg cacatcccgg cggagtacgg
     2341 cggcgccggc ctcggggtcg tggacggctg catcctggcg gaggagatcg cccgcggctg
     2401 caccggcatc ggcaccgcga tggaggcgaa cacgctcgcc gaggggccga tcatcgtcgg
     2461 cgcgtccgac gagcagaagc gccgcatcct gaccccgatg accgaggagt tccgcctcgc
     2521 cgcctactgc gtcaccgagc cgcaggccgg atcggacgtg gccgggatga agacgacggc
     2581 ccgcaagacg ggagacgact acgtcctcaa gggtcagaag atgtggatca ccaacggcag
     2641 cgtcgccgac tggtatttcg tcgtcgccta caccgacgaa gcgcagaagc accgcggcat
     2701 gagcgccttc tacgtgccac gcgacgcccc cggcattgag gtcggcaaga aagagaagaa
     2761 catggggcag cgcgcctccg acacgcgcgc catcaccttc aacgatgtgg tggtgccgaa
     2821 gaaggacatg atcggcgccg aggggaaggg gtggctgctg gccatggcgg cgttcgacca
     2881 ctcgcggccg atcgtcgccg cggccgccgt cggcctggcc cgcgccgcca tggagcacgc
     2941 cgcccgctac gcgaaagagc ggaccgcctt cggcgtcccg atctacaagc accagacgat
     3001 cgccttcatg gtcgcggaga tggcgatgaa gatcgaggcg gcgcgcctgc tcgtctggca
     3061 gtcggcctgg aagatcgacc gcggcgagcg caacacccgc gaggcggcca tggccaaggc
     3121 gttcgccgcc gactccgcca tgcaggtggc gaccgacgcc gtgcaggtct tcggcggcta
     3181 cggcttcagc aaggagtacc cggtcgagaa gctgatgcgc gacgccaaga tcttccagat
     3241 ctacgaaggg acctcgcaga tccagcgcct catcatcgcc cgcgagatct tcgagcgcgg
     3301 ctgagccggg gcctttccct ccccccgctt cggttttcgc ttgcgcgacc gaacctttgg
     3361 gggtagtcga acgactaacc cctccaaggg agaaggtgtg ccaatgggtc gcaaaaggag
     3421 accgggcgcc tggatcgccg tcatcgcggt cctgccgctc ggactcgtcg ccgcgggcgc
     3481 tccaggcgaa gtcgccgggg ggaaggggga caagcgggcc ggtgtgaccg gcgccccagc
     3541 ggtcgagcgg cgcgggacac cggtcctcgg accccggggc aggcgcgaga cggtgaccga
     3601 catcatggac cgggagcgtt tccgcaagtc cgagccgggc cgcgatgacg tggaaaggga
     3661 ggaggcgcgc ctccggccgg ttcctcccag gacgaacccg gacgcccccg gggtcagcgc
     3721 c
//
LOCUS       DASRIQ010000400         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_695959, whole genome shotgun sequence.
ACCESSION   DASRIQ010000400 DASRIQ010000000
VERSION     DASRIQ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695959"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2197)
                     /gene="recJ"
                     /locus_tag="VFD06_09795"
     CDS             complement(<1..2197)
                     /gene="recJ"
                     /locus_tag="VFD06_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZN03863.1"
                     /translation="MPESAHAFAAEPYDYADVRAIAADLDLSEPVAVTLVRRGYNTPE
                     LAREFLAADESHPPQAFERMEEVVALVMAAVVEGKRITVHGDFDVDGVCATSVMVSTL
                     RALGTDCDWFIPDRMEDGYGLSAANVERLAERGTELLITVDCGITAVEEVALAKQRGI
                     EVVVTDHHQQAEALPDCSILHPEVSGYPFEALCGTAVAWKLACALRGDGVEADLDLVA
                     LATVADVVPLVGENRSLVKRGLAEMRRAQRPGIRALIAAAKCEPTQLDEGDLAFRLAP
                     RINAAGRLYRADAGVELMLTEDEERAEQIAAELSRANSERRATEREVDTAAEAARREL
                     PEDLKEAPGFVVAGEGWHPGVVGIVASRLVERHNRPVVVISLDEEGMGRGSGRSIPGF
                     DLLGALQACAEHLESFGGHRAAAGLSLRAENLEAFRAAFAAHASSVLGPEDLQRTERI
                     DAMVGGVGLGLDLAEELGKLGPFGMGNPGVRLMVPQARVSDVRTMGAEGKHARFSLHS
                     GSHRALGVAFGRSSLGVEPEDAVDAAVRLEVNHWNGSVEPRLVLRELYPLESGVEESA
                     PAHKCACEDAEWWRRFEAELHRDLAAPDQGLATSSPTSVSNSTREVVSGGAPISAALA
                     ELVSSGAGVLAVCADASRRAALAGGASGLARFNGGSGRVACHNCGSAAIAGVIAKASG
                     GLALVDYATLAAQSQNVVECFEHVVLVDPPPTPLAADLVSAPREEWTDDD"
BASE COUNT          349 a          909 c          699 g          307 t
ORIGIN      
        1 cgtcgtcgtc ggtccactct tcgcggggcg cggagacaag gtcggcagcc agcggcgtcg
       61 ggggcgggtc gacgagaacg acatgctcga agcactcgac cacgttctgc gactgcgcgg
      121 cgagcgttgc gtagtcgacc aacgccagac cgccgctcgc cttcgcgatc acgcccgcga
      181 tcgccgcgct cccgcagttg tgacaggcaa cgcggccgct accgccgttg aagcgcgcca
      241 gccccgaggc gccgcccgcg agcgccgccc gccgcgaggc gtcggcgcag accgcaagca
      301 ccccggcgcc gctcgaaacg agctccgcca gcgcggcact gatcggggcg ccgccgctca
      361 cgacctcgcg tgtcgagtta ctcacggacg tgggtgagga agtcgccaaa ccctgatcgg
      421 gcgcggcgag gtcgcggtga agctcggctt cgaagcggcg ccaccactcc gcgtcctcgc
      481 aggcgcactt gtgcgccggg gcgctctcct ccaccccgct ctccagcggg tacagctcgc
      541 gcaggaccaa acggggctcg acggagccgt tccagtggtt gacctcgagc cgcaccgccg
      601 cgtcgaccgc gtcctcgggc tcgacgccca gggaggagcg accgaaggca acaccgagag
      661 cgcggtgtga accgctgtgc aggctgaagc gcgcgtgctt gccctcggcg cccatcgtgc
      721 gcacgtcgct gacccgcgct tggggaacca tcagccgcac cccggggttg cccatcccga
      781 acggtccgag cttgccgagt tcctcggcga ggtcaaggcc gaggcctacc ccaccaacca
      841 tcgcgtcgat ccgctcggtg cgctgcaaat cctcgggtcc caacacggag ctcgcgtgcg
      901 ccgcgaacgc ggcgcggaac gcctccaggt tctcggcccg cagcgacagc cccgccgcgg
      961 cccggtgccc gccgaagctc tccaggtgct cggcgcacgc ttgcagcgcc cccaaaaggt
     1021 cgaagccggg aatgctgcgc ccgctccccc gccccatccc ctcttcatcc agcgagatca
     1081 caaccaccgg gcggttgtgt cgctcgacca gtcgcgaagc gacgatcccg accacgccgg
     1141 gatgccagcc ctcgccggcg accacaaagc ccggggcctc tttcagatcc tccggcaact
     1201 cccttcgcgc cgcctccgcc gcggtatcga cctcgcgctc ggtcgcgcgc cgctccgagt
     1261 tcgcccggct cagctcggcc gcgatctgtt ccgcccgctc ctcgtcctcg gtcagcatca
     1321 gctccacccc ggcgtccgcc cggtacagcc gccccgccgc gttgatccgc ggcgccaagc
     1381 ggaacgccag gtccccctcg tccaactgcg tcggctcgca tttcgccgcc gcgatcagcg
     1441 cccggatccc cggccgctgc gcccgccgca tctccgccag gccgcgcttc accaaggacc
     1501 ggttctcccc caccagcggc accacatcgg caaccgtcgc cagcgccacc aagtccagat
     1561 cagcctccac cccatccccc cgcaacgcac aagccaactt ccaggccacc gccgtcccac
     1621 acagagcctc gaacgggtag ccgctcactt ccgggtgcaa gatcgagcaa tccggcagcg
     1681 cctccgcctg ctgatggtga tcggtgacca cgacctcgat tccacgctgc ttggccagcg
     1741 ccacctcctc gaccgccgtg atcccgcagt cgaccgtgat cagcagctcc gtcccccgct
     1801 ccgccaggcg ctccacgttc gccgcgctca acccgtagcc gtcctccatc cggtcgggaa
     1861 tgaaccagtc gcaatcggtg ccgagtgcgc gcagggtgga gaccatcacc gaggtcgcgc
     1921 agaccccgtc gacgtcgaag tcgccgtgca cggtgatccg cttgccctca acgaccgccg
     1981 ccatgaccag cgccacaacc tcctccatcc gctcgaacgc ctgcggcggg tgcgactcgt
     2041 cggcggcgag gaactcgcgc gccagctccg gcgtgttgta gccgcgccgg accagcgtca
     2101 ccgcgaccgg ctcgctcagg tcgaggtcag cggcgatggc acgaacgtcg gcgtagtcgt
     2161 agggctcggc ggcaaatgcg tgggcggatt ccggcacatg cccaggctag gcagcggcgc
     2221 ggcgggaaac ccgctgccga tcctgcctaa attgcaggga gatc
//
LOCUS       DASRIQ010000401        10765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1474672, whole genome shotgun sequence.
ACCESSION   DASRIQ010000401 DASRIQ010000000
VERSION     DASRIQ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10765
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474672"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFD06_09800"
     CDS             <1..711
                     /locus_tag="VFD06_09800"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HZN03864.1"
                     /translation="AGLIRNCGVPKTPSPDLIKTAYALYRRRDDASHPRGIEARLFRR
                     MHAHETRIRCIGVWDTVGALGIPGGGFRWLNRRWAFHDVDLSSTVDNAFHALAADEER
                     KVFVPSLWRRQPGSNGQRLEQVWFAGAHSNIGGGYADPGLSDVAFLWMKQRAEECGLD
                     FDAAVVRDATRPDPLGELRDSSVGVYRFLPRRRRPIGETPGANESVHGSVPARLAGVP
                     GYRPENVLAFLRRTRAAA"
     gene            764..1927
                     /locus_tag="VFD06_09805"
     CDS             764..1927
                     /locus_tag="VFD06_09805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN03865.1"
                     /translation="MRKASVVIVGAGIMGASAAWHLAARGWRDILLLDRSPGPGEGST
                     GKATGGFRAQFETAVNVRLSLLSRAALRSFPEEIGVDSGYRPFGYLWIARHPAQMEAL
                     AAARRVQHAEGLHEAAAVTPGDIARINPAVSPEGILGGAFCPTDGFLRARAMLDGYLA
                     AAARLGARVEWGVEVTGFARDAEGRIATVRTVKESIAAGAVVNAAGAWAAETARAAGI
                     DLPVAPLRRQVALTAPTDLLAEEMPMTIFLEDGFHLRVRDGRVLLLRPTPGVPGRPLE
                     TTVDDDWVREVAATGVERVPVLRNVPVDRSACWAGLYEMSPDRHAILGRAPGCPNLFL
                     INGSSGHGVMHAPALGLLLAEILSDGRARTIDATPLDPDRFAAATGAKPAGLL"
     gene            1929..4160
                     /locus_tag="VFD06_09810"
     CDS             1929..4160
                     /locus_tag="VFD06_09810"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZN03866.1"
                     /translation="MPPTLNAFGLRLSAPALVGAGVLVVALLAAAAWKFAAPRPVPMP
                     ERLRAIAAATDPETNMFQNGDRAKALEARWTREGEAAGPGVLMGLARELLNAGRTEEA
                     IRRFDELETQTRRRGVVPGSGPWIELRVQQAIAQLRRAEVENCAAMHNPRSCLFPIEG
                     AGVHGRTRGSEAAMTLLTDVLNASPDHQQARWLLNIAAMTLGVWPDGVPERWRLGPEL
                     FASEHDIGRFPDIAGRLGLDVDDHAGGSIVDDLDGDGDYDIMASAMGPASPLRFFVNQ
                     GDGTFVERTKEAGLDGENGALNIIQGDYDNDGRIDVLMLRGGWMGRAGRLPVSLLHND
                     GGGRFTDVTEEAGLLRFHPTQTAVWFDYDGDGWLDLFIGNEARQDDPHPCELFHSNRD
                     GTFTEVAAQAGVAAVGFVKGVTSGDFNNDGRPDLYLSVRGAENILYRNDGASGGGWRF
                     TDVAAEAGVTEPFVSFPTWFFDYDNDGWEDLFVSGYLIRNAGDLAADALGLATEAERP
                     RLYRNNGDGTFADVTRAAHLHRVLQSMGSNYGDLDNDGWLDIYLGTGDPELGTLIPDR
                     VFRNAEGRRFQDVTTSGGFGHLQKGHGVSFADLDNDGDQDVYHVVGGAFEADTFRNAL
                     FENPGHGNRFLALQLEGVKANRGAIGARLRVVVTTAAGERSLHRTVRSGGSFGCSPLR
                     QEIGLGDATGVARVEIRWPGSDGLEVLTGLEPDRRYLIRQGESAARPLSLAPVRLGGK
                     ERS"
     gene            4487..5410
                     /locus_tag="VFD06_09815"
     CDS             4487..5410
                     /locus_tag="VFD06_09815"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4670..4672,aa:Sec)
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HZN03867.1"
                     /translation="MYRPLQAAAFTGADGAHGVEEDDLVLGVTAGGEASAYPVRALAY
                     HHLVNDTVGGRALVATYUTLCHTGLVWSATVEGRTLHFRLAGINNQNFIMRDDETGTW
                     WQQITGCALDGPLEGRCLDAVAWDEVTLAVWRHEHPATRVLLPDAGRVEEYAEAGWEA
                     KVDELPLVTPVDPEDLLAPRDLVVGLTVGGAATAFPWKDLSARGVLAGRVAEQPVLLV
                     LHPDGRSLRCFDPRAGGRALDLFRPPCGGPPLAESEVLRDRETGSGWDFQGKARSGPL
                     AGTILAGIPCLKDYWFDWKLYHPGTGVAGAP"
     gene            complement(5414..5620)
                     /locus_tag="VFD06_09820"
     CDS             complement(5414..5620)
                     /locus_tag="VFD06_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03868.1"
                     /translation="MSKRSQSSFMKRQKEIARQERRQDKLARKLERNKQRENASSGRP
                     EGIDPDIAGIVPGPQPPSEDYPDR"
     gene            complement(5660..6043)
                     /locus_tag="VFD06_09825"
     CDS             complement(5660..6043)
                     /locus_tag="VFD06_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZN03869.1"
                     /translation="MGTRLFVGNLSFDVSDDDLRQAFSEAGSCSSASIVRDRMTGKSR
                     GFGFVEMTTEEEAKRAVSTLNGRDLQGRAMNVSEARERGAGPSAPRMSSFRSFGSSGG
                     GSPEPPPYRKEGGSRRGLRAKKRSL"
     gene            6185..6967
                     /locus_tag="VFD06_09830"
     CDS             6185..6967
                     /locus_tag="VFD06_09830"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN03870.1"
                     /translation="MRGMPAPGGGDRFEAWVSALEARHLADLRFPEVRRGLQALSSLY
                     VSRRARLGEGAALDGAGKRAAFALFYAPIHFLLTRAIVRALEAARPGPRSLVDLGCGT
                     GAAGAAWALEIRGRCRLQAIDRSGWAIDEARRTFEFFGLEARAARGSLLEVPLPGRGG
                     AILLAFAANELDEADRGRLLARLLEASGRGAAVLVIEPIARRPASWFGAWRAAFAPQG
                     GRADEWRFPAALPESIRLLDRAAGLDHRELTARSLWLPASRR"
     gene            7002..8231
                     /locus_tag="VFD06_09835"
     CDS             7002..8231
                     /locus_tag="VFD06_09835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN03871.1"
                     /translation="MFLPIGDSPNPPGRPIVTWALILLNVAAYVWLLPLSFRQADPQD
                     PAFAAYLQAIADDRRLSLPEVHMVARGMSEYDLLVFRHGFHPATPTLAGLLTSMFLHG
                     GFMHLFGNMLFLWIYGDNVEYRMGRILYLMAYLGTGAVAGLGDALIRMGSNLPEVGAS
                     GAISGVLGLYFVWFPYNRVRTWVFLFPLFVNVIELPARLVLGVFVLIDNLLPLVLGAE
                     GGVSHGAHIGGFVAGAGLALATGRLSLRGTVPAGRGEADEPASPAGEAAAARRVSPMR
                     PLGGSGLRDALDDGRWEEAARAFFDAPSSRTRTVGPWEKIRLGDALSEHGHPRPALAA
                     YQRALVDHPSGPGRAAAHLGAARVLMDALDNPTGAYQHLYGALEERPDEAEEAEARAL
                     LDRLRGVMRHVPGRRPI"
     gene            8276..10630
                     /locus_tag="VFD06_09840"
     CDS             8276..10630
                     /locus_tag="VFD06_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03872.1"
                     /translation="MSRRLVLALGAVLAAAVLVAGVLLLVRRGPDSSGGDSGGRDVAR
                     SVGFDLPATAGAADARRMDALRQAAASESSPAEVTIDYPQDQSIVPPELPPPTFLWHD
                     GSAQADAWLAEVTFEGGSERIDVLVKGVPPPSGEVDPRCISERNEIYRPTPYQASARS
                     WKPDPVLWQALKERAASSPATVALYGFRQAEPGRVLSKGRMRLSTSADPVGAPIFYRD
                     VPLMPSQTEEGVIKPLAKGALPLITWRLRDVAKTESRAMLTDMPTCANCHSFSNDGRT
                     MGMDVDGPQGDRGAYALVPVSDRVSIGYRDVLTWNAFPGKPKDFMTIGFLSRVSPDGR
                     HVISTVNESLYVQNFWNYKFNQVFYPTRGILAVYSRATRAIASLPGADDPAFVHCDPV
                     FSPDGKTVVFARAKARDAYVPGRPLATYAGDPNETPIQYDLYRVPFEDGRGGEAEPIE
                     GAGRNGMSNSFPKVSPDGRFIVFVQAKNGQLMRPDSKLYIVPFEGGTARLMRCNLPLM
                     NSWHSYSPNGRWMVFSSKSNTPYTQMFLTHIDAEGNDSPAILIENSTAANRAVNLPEF
                     VAAPYDRFNGIDVPAVDHTRHFNRGTDLALKGEHEEAVAEFKAALGTEPNDWKTSEWR
                     IRESLSKSLLMLGRHEEAYREVTESLRLNPYNGEMHANLAAFLFEAGDKERAFQHLDL
                     AIRLMPKSALPLYNRATMRLKGGDRAGALADYGEAIRLDPNDPASWYGRGLVRADAGD
                     SGGALQDLTEALRVAPADWPRRSDAEARRRQLRSALSGSGQRGR"
BASE COUNT         1427 a         3843 c         3891 g         1604 t
ORIGIN      
        1 gccggcctga tccgcaactg cggcgtgccg aagacgccgt cgcccgatct catcaagacg
       61 gcgtacgccc tgtaccggcg gcgcgacgac gcgtcgcacc cgcgcggcat cgaggcgcgg
      121 ctcttccgac gcatgcatgc ccacgagacg cgcatccggt gcatcggcgt ctgggacacg
      181 gtcggcgcgc tcggaatccc ggggggcggc ttccgctggc tcaaccgccg ctgggccttc
      241 cacgacgtcg acctgagcag cacggtggac aacgccttcc acgcgctggc cgccgacgag
      301 gagcgcaagg tgttcgtccc gtccctctgg agaaggcagc cggggtcgaa cggacagcgg
      361 ctggagcagg tgtggttcgc cggggcgcac tcgaacatcg ggggtggcta cgccgacccc
      421 ggcctgtccg acgtggcgtt tctctggatg aagcagcgcg ccgaggagtg cggcctggac
      481 ttcgacgcgg cggtggtgcg cgacgcgacg cgcccggacc cgctggggga gctgcgcgat
      541 tcctcggtcg gggtctaccg cttcctgccg cggcggcgcc gtccgatcgg cgagacgccg
      601 ggagcgaacg agtcggtgca cgggagtgtc ccagcgcgcc tcgccggcgt tccgggctac
      661 cggccggaga acgtcctggc gttcctgcgc cggacgcggg ccgcggcgta ggacgcgcgg
      721 gctgaccgcc gcgcggacag gctcccgggc gtagactccc gggatgcgga aggcctcggt
      781 cgtcatcgtc ggcgccggaa tcatgggagc cagcgccgcc tggcatctcg cggcgcgcgg
      841 ctggcgcgac atcctgctgc tcgatcgcag tccgggcccc ggcgagggga gcaccgggaa
      901 ggcgaccggc ggcttccggg cgcagttcga gaccgccgtc aacgtccgcc tgtccctgct
      961 gtcgcgcgcg gcgcttcgga gcttccccga ggagatcggc gtcgattccg gctaccggcc
     1021 gttcgggtat ctgtggatcg cgcgccatcc cgcgcagatg gaggcgctcg ctgccgcccg
     1081 gcgcgtccag cacgcggagg ggcttcacga agcggcggcc gtcaccccgg gcgacatcgc
     1141 ccggatcaac ccggccgtct ctccggaagg gatcctcggc ggcgccttct gccccacgga
     1201 cgggttcctg cgcgcccgcg ccatgctcga cggatacctc gccgccgcgg cgcgcctcgg
     1261 cgcccgcgtc gaatgggggg tcgaggtcac cgggttcgca cgcgatgcgg aaggccggat
     1321 cgcgaccgtt cggacggtga aggagtcgat cgccgccggc gcggtcgtca acgccgcggg
     1381 ggcgtgggcc gcggagacgg cgcgcgcggc cgggatcgat ctcccggtcg cgccgctgcg
     1441 gcgccaggtg gccctgacgg cgccgaccga tctcctcgcg gaggagatgc cgatgacgat
     1501 cttcctcgag gacggcttcc acctccgggt gcgcgacggg cgggtgctcc tcctccggcc
     1561 gacgccgggc gttccggggc ggccgctcga gaccaccgtg gacgacgact gggtgcggga
     1621 ggtggccgcc acgggggtgg agcgggtgcc ggtgctgcga aacgttccgg tggatcggag
     1681 cgcctgctgg gccggactgt acgagatgtc gccggatcgc cacgccatcc tcggccgcgc
     1741 ccccggatgc ccgaacctgt tcctcatcaa cggctcctcg gggcacggcg tgatgcacgc
     1801 gccggctctc ggcctgctcc tggccgagat cctctcggac ggtcgagcgc gcaccatcga
     1861 tgcgacgccg ctcgaccccg accgcttcgc cgccgccacc ggggcgaagc ctgcgggact
     1921 cctttaacat gccgcccacc ttgaacgcct tcggactgcg cctgagcgcg ccggccctcg
     1981 tgggcgccgg cgtcctcgtc gtcgccctcc tcgcggccgc ggcgtggaag ttcgccgcac
     2041 cgcgccccgt tccgatgccg gagcggctgc gggccatcgc cgccgcgacc gatcccgaga
     2101 cgaacatgtt ccagaacggg gaccgcgcga aagcgctcga ggcccgctgg acccgggaag
     2161 gggaggcggc ggggccgggc gtcctcatgg ggctcgcccg agagctgctc aacgcgggtc
     2221 ggaccgagga ggccatccgg cgcttcgacg agctcgagac gcagacgcgc cgccgcggtg
     2281 tcgtgccggg atccggcccg tggatcgagc tgagggtcca gcaggcgatc gcgcagctgc
     2341 ggcgggccga ggtggagaac tgcgccgcca tgcacaaccc gcgttcgtgc ctgttcccga
     2401 tcgagggggc gggggtgcac gggcgcacgc gcggatcgga ggcggcgatg acgctgctca
     2461 cggacgtgct gaacgcctcc cccgatcacc agcaggcgcg ctggctcctc aacatcgccg
     2521 cgatgacgct cggcgtctgg ccggacggcg tgccggagcg ctggcgcctc ggtccggagc
     2581 tcttcgcctc cgagcacgac atcggccggt tccccgacat cgccgggcgg ctgggactgg
     2641 acgtcgatga tcacgccggc ggatcgatcg tggacgacct cgacggtgac ggtgactacg
     2701 acatcatggc ttcggcgatg ggaccggcgt cgccgctgcg cttcttcgtc aaccagggcg
     2761 acggcacctt cgtcgagcgg acgaaggagg ccggcctcga cggggagaac ggcgccctca
     2821 acatcatcca gggggactac gacaacgacg gccggatcga cgtcctgatg ctgcgcggcg
     2881 ggtggatggg ccgcgccggc cggctgccgg tctcgctgct gcacaacgac ggcggagggc
     2941 gattcaccga cgtcaccgag gaggcggggc tcctgagatt ccatccgacg cagaccgccg
     3001 tctggttcga ctacgacggc gacggctggc tcgacctctt catcggcaac gaagcgaggc
     3061 aggacgaccc ccacccctgc gagctgttcc acagcaaccg ggacggcacc ttcacggagg
     3121 tggcggcgca ggccggcgtc gccgcggtgg gcttcgtgaa gggggtgacg agcggcgact
     3181 tcaacaacga cgggcgcccc gatctgtacc tgtcggtgcg cggcgcggag aacatcctgt
     3241 accgcaacga cggggcctcg ggcggcggct ggcgattcac cgacgtggcg gcggaggcgg
     3301 gggtgaccga gccgttcgtc tctttcccga cctggttctt cgactacgac aacgacgggt
     3361 gggaggacct cttcgtctcc ggctacctca tccgcaacgc cggcgatctc gccgccgacg
     3421 ccctcgggct ggcgaccgaa gcggagcgcc cgcgcctgta ccgcaacaac ggcgacggga
     3481 cgttcgccga cgtgacgcgg gcggcgcatc tccaccgcgt cctgcagtcg atggggtcga
     3541 actacggcga cctcgacaac gatggctggc tcgacatcta tctcggcacc ggcgacccgg
     3601 agctcggcac gctcatcccc gaccgggtct tccgcaacgc ggaggggcgc cgcttccagg
     3661 acgtcaccac ctcggggggg ttcggccatc tccagaaggg gcacggcgtc tcgttcgccg
     3721 atctcgacaa tgacggcgac caggacgttt accacgtcgt cggcggcgcc ttcgaagcgg
     3781 acactttccg caatgccctg ttcgagaatc ccgggcacgg gaaccgcttc ctggcgctgc
     3841 agctcgaggg ggtgaaggcg aaccgcggcg ccatcggcgc gcgcctgcgc gtggtggtca
     3901 ccaccgccgc gggggagcga tccctgcacc ggacggtgcg gagcggcggg tcgttcggct
     3961 gctcgccgct gcggcaggag atcggtctcg gcgacgcgac cggtgtcgcg cgcgtcgaga
     4021 tccgctggcc ggggagtgac ggcctcgagg tgctgaccgg cctcgagccg gaccggcgct
     4081 acctcatccg gcagggggag agcgccgcgc ggccgttgtc gctcgcgccg gtccggctcg
     4141 gcgggaagga gcgatcgtga tcgcgccggg cggtgcgcgt cgtccgggcg ccgtcgccgc
     4201 gtggatcctg ctcggcgccg ccgtcgcgct gtcgctcctc ctcgtgctga cgccggcggg
     4261 cctcctgatg ccgttccgtg cacagaccgc ggacgaggtg cggctttcat acgttctgag
     4321 gtcgtggtcc ccccgcgtga ccccgattct cctggggctc ggggtggcgg ccgcggtcct
     4381 cctttggagt cgcacccgct cgcgctggct caaggggatc gcgtgcctgg cggtcgcgct
     4441 gctcgccggt tccgcatggc tggcgcggca gaattatttc gagtggatgt accgtccgct
     4501 ccaggccgcg gccttcaccg gagccgacgg cgcccacggc gtggaggaag acgacctggt
     4561 gctcggcgtg acggcgggag gggaggcgag cgcctatccc gtgcgcgccc tcgcctacca
     4621 ccacctggtc aacgacacgg tcggcgggcg cgcgctcgtc gccacctatt gaacgctgtg
     4681 ccacaccggt ctggtgtgga gcgcgacggt cgagggaagg acgctgcatt tccgtctcgc
     4741 cggcatcaac aaccagaact tcatcatgcg cgacgacgag accggcacct ggtggcagca
     4801 gatcaccggc tgcgcgctcg atgggccgct cgagggtcga tgcctcgatg cggtggcgtg
     4861 ggacgaggtg acgctcgcgg tctggcggca cgagcacccg gcgacgcgcg tgctgctgcc
     4921 ggacgccggg cgcgtcgagg agtacgccga ggcaggctgg gaggcgaagg tcgatgagct
     4981 tccgctggtc actccggtcg accccgaaga cctcctggcg ccgcgtgatc tggtcgtcgg
     5041 gctcacggtc ggcggcgcgg cgacggcctt tccctggaag gatctttccg cgcgcggcgt
     5101 cctcgccggc cgggtggcgg agcagccggt gctcctggtc ctgcatcccg atggccgatc
     5161 gctgcgctgt ttcgatcccc gcgccggcgg gcgcgcgctc gacctgttcc ggccgccgtg
     5221 cggcggcccg cccctcgcgg agtcggaggt gctgcgcgat cgggagaccg gcagcggctg
     5281 ggacttccag gggaaagcgc gctccgggcc gctcgcgggg acgatcctcg ccgggatccc
     5341 ctgtctcaag gactactggt tcgactggaa gctgtaccac cccgggaccg gggtggccgg
     5401 cgcgccctga cccctatcga tcgggatagt cctcggacgg gggctgcggt cccgggacga
     5461 tgccggcgat gtccggatcg atcccttcgg gccggccgga ggaggcgttc tcccgctgct
     5521 tgttcctctc gagcttgcgg gcgagcttgt cctggcgccg ctcctggcgg gcgatctctt
     5581 tctggcgctt catgaagctg gactgcgagc gtttcgacat ggtgcgacct cgtggttccc
     5641 ggtgacgacg gctacgtgac tacagacttc gtttcttcgc cctcagaccg cgacgactgc
     5701 cgccctcctt gcggtacggg ggcggctccg gcgatcctcc gccggaagag ccgaagctgc
     5761 ggaagctcga catgcggggc gcggacggac cggcgccgcg ctcgcgcgcc tcgctgacgt
     5821 tcatcgcccg gccctggaga tcacggccgt tgagggtcga gaccgccctt ttggcctcct
     5881 cctcggtcgt catctccacg aacccgaagc cgcgcgactt gccggtcatc cgatcgcgca
     5941 cgatggacgc cgacgagcac gagccggctt ccgagaacgc ctggcgcaga tcatcgtccg
     6001 agacgtcgaa tgacagattt cccacgaaca gcctcgtccc catcctcgct ccttccgcgg
     6061 gcctatcttc catcgccgca tttgcgacga atgaatcggt ttggactgat cggttccgca
     6121 gggaagtcta accgcacaag cgggagatcg tcaacgaacg cggccgcgcc attgccgcga
     6181 tagcatgcgc ggtatgcccg cccccggagg aggcgatcgg ttcgaggcat gggtctccgc
     6241 cctcgaggcg cggcacctcg ccgatctcag gttccccgag gtgcggcgcg gactgcaggc
     6301 gctgtcgtcg ctctatgtgt cccgccgcgc gcggctgggg gagggggcgg cgctcgacgg
     6361 ggcggggaag cgggccgcgt tcgccctgtt ctacgcgccg atccatttcc tcctgacccg
     6421 cgcgatcgtg cgcgccctcg aggcggcgcg acccggcccg aggtcgctcg tcgatctggg
     6481 ctgcggcacc ggcgcggccg gagccgcctg ggcgctggag atccgcggcc gctgccggct
     6541 gcaggcgatc gatcgcagcg gctgggcgat cgacgaggcg cgccgcacct tcgagttctt
     6601 cgggctcgag gcgcgcgccg cgcgcggctc gctccttgag gtcccgctgc ccggccgggg
     6661 cggcgcgatt ctcctggcct tcgcggccaa cgagctcgac gaggccgatc gcggccgcct
     6721 gctcgcccgg ctgctcgagg cttcggggcg cggtgccgcg gtcctggtga tcgagccgat
     6781 cgcgcgccgc cccgcgtcgt ggttcggcgc gtggcgcgcg gccttcgcgc cgcagggggg
     6841 gcgggccgac gagtggcggt tcccggcggc gctgcccgag agcatccggc tcctcgatcg
     6901 ggccgcgggt ctcgatcacc gcgagctcac ggcgcggagc ctctggcttc ccgcgtcgcg
     6961 gcgctgacgc ccgggtcacg gtgggataga gttcccggga cgtgttcctt cccatcggcg
     7021 acagcccgaa cccgcccggg cgtccgatcg tcacgtgggc gctcatcctc ctgaacgtcg
     7081 cggcgtacgt ctggctcctg ccgctgtcgt tccggcaggc ggatccgcag gacccggcgt
     7141 tcgccgccta tcttcaggcg atcgccgacg accgcaggct gtcgctgccg gaggtccaca
     7201 tggtggcgcg ggggatgagc gagtacgacc tcctcgtctt ccgccacggg ttccatcccg
     7261 cgaccccgac gctcgccggg ctgctcacct cgatgttcct gcacggcggg ttcatgcacc
     7321 tgttcgggaa catgctgttc ctgtggatct acggtgacaa cgtcgagtac cggatggggc
     7381 ggatcctgta cctcatggcg tatctcggca cgggcgcggt cgcgggcctg ggggacgcgc
     7441 tcatccgcat gggatcgaac ctgccggagg tgggcgcgtc gggggcgatc tccggggtgc
     7501 tcggtctcta cttcgtctgg ttcccgtaca accgggtgcg cacctgggtc ttcctttttc
     7561 cgctcttcgt gaacgtcatc gagctgccgg cgcggctcgt cctcggcgtg ttcgtcctca
     7621 tcgacaacct gctgccgctc gtcctcggcg cggagggggg cgtgtcgcac ggcgcccaca
     7681 tcggcggctt cgtcgccggc gccggcctcg ccctcgcgac agggaggttg tcgctgcgcg
     7741 ggacggtccc ggccgggcgc ggggaggccg atgagccggc gtcccctgcg ggggaggccg
     7801 ccgcggcgcg ccgggtgtct ccgatgcggc ctctcggcgg cagcggtctg cgggacgcgc
     7861 tcgacgacgg gcgctgggag gaggcagccc gggccttctt cgatgcccct tcgtcgcgca
     7921 cccggaccgt gggcccgtgg gagaagatcc gtctcggcga cgcgctgtcc gagcatggcc
     7981 acccgcgccc ggccctggcc gcctatcagc gggcgctcgt cgaccatccg tccggcccgg
     8041 ggcgggccgc ggcgcatctg ggcgccgcgc gggtcctgat ggacgcgctc gacaatccga
     8101 ccggcgccta ccagcacctc tacggcgcgc tcgaggagcg gcccgatgag gcggaggagg
     8161 cggaggcgcg cgcgctcctc gatcgcctgc gcggcgtgat gcgccacgtg cccggccgaa
     8221 ggccgatctg aggcggcgct ttacttcgcc gccgccgctc ggggacaatc ggtccatgag
     8281 tcgccgcctc gtcctcgccc tcggggcggt ccttgccgcg gccgtactcg tggccggagt
     8341 gctcctgctc gtcaggcgcg gccccgactc ctccggcggg gattcggggg ggcgcgacgt
     8401 cgcacggagc gtcgggttcg acctcccggc gaccgcgggc gccgccgacg cgcggcgcat
     8461 ggacgccctg cggcaggcgg ccgcctcgga gtcctccccg gccgaggtga ccatcgacta
     8521 cccccaggac cagtcgatcg ttcctcccga gctgccgccg ccgaccttcc tctggcacga
     8581 cggctcggcg caggccgacg cctggctggc cgaggtgacc ttcgaagggg ggagcgagcg
     8641 gatcgacgtg ctggtgaagg gcgtcccgcc gccgtcgggt gaggtggacc cgcgttgcat
     8701 ctccgagcgc aacgagatct accggcccac gccgtaccag gcatccgccc gctcatggaa
     8761 gccggacccg gttctgtggc aggcgctcaa ggagcgggcg gcgtcgagcc ccgcgaccgt
     8821 ggccctctac ggcttccggc aggcggagcc gggacgggtg ctgtcgaagg ggcgcatgcg
     8881 gctcagcacc tcggcggatc cggtcggcgc gccgatcttc taccgcgacg tgccgctgat
     8941 gccgtcgcag accgaggagg gggtcatcaa gcccctcgcc aagggggcac tgccgctcat
     9001 cacctggcgc ctgcgcgacg tcgcgaagac cgagagccgc gccatgctca ccgacatgcc
     9061 gacctgcgcc aactgccact ccttctccaa cgacggccgc accatgggga tggacgtcga
     9121 cgggccgcag ggggatcgcg gcgcctacgc cctggtgccg gtgtccgacc gcgtgtcgat
     9181 cggctaccgg gacgttctga cctggaacgc cttcccgggg aagccgaagg acttcatgac
     9241 catcggcttc ctgtcgcgcg tcagtcccga cggccggcac gtgatctcga cggtcaacga
     9301 atcgctctac gtgcagaact tctggaacta caagttcaac caggtcttct acccgacgcg
     9361 cggcatcctc gcggtgtact cgcgcgcgac ccgcgccatc gcgtcgctgc cgggggccga
     9421 cgatcccgcg ttcgtgcact gcgacccggt cttcagcccg gacgggaaga ccgtggtgtt
     9481 cgcgcgcgcg aaggcgcgcg acgcctacgt gccgggacgc ccgctcgcca cctacgccgg
     9541 cgatccgaac gagacgccga tccagtacga cctgtaccgc gtcccgttcg aggacggccg
     9601 cggcggggag gcggagccga tcgagggggc ggggcgcaac ggcatgagca actccttccc
     9661 caaggtgtcg cccgacggcc gcttcatcgt cttcgtgcag gcgaagaacg gccagctcat
     9721 gcgccccgac agcaagctgt acatcgtgcc gttcgaaggg ggaacggcgc gcctgatgcg
     9781 ctgcaacctg ccgctgatga actcctggca ctcgtactcg ccgaacgggc gctggatggt
     9841 cttctcctcg aagagcaaca cgccgtacac gcagatgttc ctgacccaca tcgacgcgga
     9901 ggggaacgac agcccggcga tcctgatcga gaacagcacc gccgccaacc gcgcggtgaa
     9961 cctgccggaa ttcgtcgccg cgccgtacga ccggttcaac ggcattgacg tgccggccgt
    10021 cgatcacacc cggcacttca accgcggcac cgatctcgcg ctcaaggggg agcacgagga
    10081 ggcggtggcg gagttcaagg ccgccctcgg caccgaaccg aacgactgga agacgagcga
    10141 gtggcgcatc cgggagagcc tgagcaagtc gctcctgatg ctcggccgcc acgaggaggc
    10201 gtaccgggag gtcaccgagt cgctccgcct caatccctac aacggcgaga tgcacgccaa
    10261 cctcgcggcg ttcctgttcg aggcggggga caaggagcgc gccttccagc acctcgatct
    10321 cgccatccgc ctgatgccga agagcgcgct gccgctctac aaccgggcga ccatgcgcct
    10381 caagggaggg gatcgcgccg gagccctggc cgactatggc gaggcgatcc gcctcgatcc
    10441 gaacgaccct gcctcctggt acggccgcgg cctggtccgg gccgacgcgg gcgattccgg
    10501 gggcgcgctc caggacctga ccgaagcgct ccgggtcgcg cccgccgact ggccgaggcg
    10561 gtcggatgcc gaggcgcgcc ggcgccagct ccgcagcgcc ctctccggct ccggccagcg
    10621 gggtcgttag gagccacagg ctcccggccg gaccccgcgt cacggcggcc gaccgccctg
    10681 accgcgaaaa aactcccccc tttccgcacc accttgtcga gacgacgggg cgccgtgcgt
    10741 cgtagggatg aacggatggt ttcgc
//
LOCUS       DASRIQ010000402         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3556670, whole genome shotgun sequence.
ACCESSION   DASRIQ010000402 DASRIQ010000000
VERSION     DASRIQ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3556670"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VFD06_09845"
     CDS             <1..1456
                     /locus_tag="VFD06_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN03873.1"
                     /translation="LVLPGFNDAHVHFLAGGLDLENVNLKDAPSREEFARRIAERAAI
                     VPAGEWVTGGNWDDQAWSPAVMPDATLVDDALRAAGKEDVPVFVSRYDGHMALANTAA
                     MRLAKVDARTKDVPGGEIGRDASGRPNGLFKDAAMSLVSRAMPPLSRERRLRAIHKAL
                     AHAASLGVTSIQDMNPEGDDINVYAELLARGELTVRVYAAPYLVRWKDYAATGVRRAF
                     GSPWLRIGALKGYADGSLGSSTAFFYEPFSDQPGSHGLLSDEETPPAAMLERMMGADA
                     AGLQLCIHAIGDQGIALVLDQFAAVAAAHGPADRRFRVEHAQHMAAKDFARFAQGGVI
                     ASMQPYHAIDDGRWAEKRIGPERIKTTYAFRSFLDAGVRLAFGTDWSVAPLDPILGLQ
                     AALTRATLDGKNPGGWVPGQKITLAEAVTAYTQGSAYAEFQEREKGILAPGMLADLVV
                     LSRDIFHLEPRDLHEARVTTTIVGGRVVYEAPAQ"
     gene            complement(1450..1650)
                     /locus_tag="VFD06_09850"
     CDS             complement(1450..1650)
                     /locus_tag="VFD06_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03874.1"
                     /translation="MKKRLLLAACTLTLVVGAIVALKPAETADKLPKCSGQLCQKSGC
                     SADTLCARGAAVVTCAEVCGGH"
     gene            1732..2478
                     /locus_tag="VFD06_09855"
     CDS             1732..2478
                     /locus_tag="VFD06_09855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN03875.1"
                     /translation="MPSTARHSLTEKNREIRANFDAIAGRYDLTNRVISCGVDLHWRK
                     VAVRTFSDLPRGGRVLDLACGTCDLSLELLRRRPDARVTGVDLSRTMLSLARPKIAAR
                     AASDRPGGVGGFPLVNAPAEALPFADGVFEGAMIGFGIRNVPDYRAGLGELRRVLRSG
                     GRLVVLEFSTPPSRLLWRAYNFYFFNVLPRIGGILTGRERAYRYLTHSVAEFPGAADF
                     GRAMEQAGFERVTWRSLTGGIVCVHTGLAA"
     gene            2475..3647
                     /locus_tag="VFD06_09860"
     CDS             2475..3647
                     /locus_tag="VFD06_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZN03876.1"
                     /translation="MKRTYTAARIEGLKQSEIRRMSLECAKVSGINLGQGICDQPIEP
                     IIKEAAIRAIDADHSIYTRLDGIDELRRRIAAKMRGYNGIVCDPDGEVVVTVGATGAF
                     VTACMAFLDPGDEVVIFSPFYSYHVNILKVCGVVLRFVTLRPPDWSYDPQALEAAFSD
                     RTKMVVLNTPANPSGKVFGKEELDHLAALCRKHDALCVTDEIYEYILYDGRRHLSLGA
                     FPGMEDRTITISGFSKTYAMTGWRLGYVVARRALASQMALLNDLINVCAPSPLQHGVV
                     AAFDLPLEYYDRMVVDYTRKRDMTAEACRAAGMEPFVPQGSYYMLADVSRLGMKDDYD
                     AARILLEQAGIAVIPGSSFYANPEDGRMQIRLCYAKKMPDLQEACRRLRAFKPVPV"
     gene            3691..>4128
                     /locus_tag="VFD06_09865"
     CDS             3691..>4128
                     /locus_tag="VFD06_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN03877.1"
                     /translation="MANLWIRKPLDKLVAEAGDSEKGLKRTLGPLNLIALGIGAIIGA
                     GLFVRTAAAIAERSGPSVTLGFIVAGIGCAFAGLCYAEFASMIPVAGSAYTYSYATMG
                     EFVAWVIGWDLVLEYAVGAATVSIAWSEYLNKLFQLFGFSIPYQ"
BASE COUNT          599 a         1478 c         1406 g          645 t
ORIGIN      
        1 cctcgtcctc cccggcttca acgacgcgca cgttcacttc ctggcgggag ggctcgacct
       61 cgagaacgtc aacctcaagg acgcgcccag ccgcgaggag ttcgcccgcc gcatcgccga
      121 gcgggccgcg atcgttccgg ccggcgagtg ggtgaccggc gggaactggg acgaccaggc
      181 ctggtcgccg gcggtcatgc cggatgcgac gctcgtggat gacgcgttgc gcgccgccgg
      241 gaaggaggac gtgccggtct tcgtcagccg ctacgacggc cacatggcgc tggcgaacac
      301 cgccgccatg cgcctcgcga aggtggacgc gcgcacgaag gacgtccccg gcggggagat
      361 cggccgcgac gcctccggcc gccccaacgg cctcttcaag gacgccgcca tgtcgctcgt
      421 gtcgcgggcg atgccgccgc tgtcgcgcga gcggcgcctg cgcgccatcc acaaggcgct
      481 cgcgcacgcg gcctcgctcg gggtgacgag catccaggac atgaaccccg aaggcgacga
      541 catcaacgtc tacgccgagc tcctggcgcg cggcgagctg accgtgcgcg tctatgccgc
      601 cccctacctc gtgcgctgga aggactacgc cgcgaccggg gtccggcgcg ccttcgggtc
      661 gccgtggctg cgcatcggcg cgctcaaggg gtacgccgac ggttcgctcg gttcctcgac
      721 cgccttcttc tacgagccgt tcagcgacca gcccggttca cacgggctcc tgtcggacga
      781 ggagaccccg cccgcggcga tgctggaacg gatgatgggg gcggacgccg ccggcctgca
      841 gctctgcatc cacgccatcg gggaccaggg gatcgcactc gtgctcgatc agttcgctgc
      901 ggtggcggcg gcgcacgggc cggccgaccg gcgcttccgc gtcgagcacg cgcagcacat
      961 ggcggcgaag gatttcgccc gcttcgccca gggcggggtg atcgcctcga tgcagccgta
     1021 ccacgcgatc gacgacggcc gctgggcgga gaagcgcatc ggcccggagc ggatcaagac
     1081 gacctacgcc ttccggtcgt tcctcgacgc cggcgtgcgc ctcgctttcg gcaccgactg
     1141 gagcgtcgcg ccgctcgacc ccatcctcgg gcttcaggcc gcattgacgc gcgccaccct
     1201 cgacggcaag aatcccggcg gctgggtgcc ggggcagaag atcaccctgg ccgaggcggt
     1261 caccgcctac acgcagggat ccgcctacgc cgagttccag gagcgcgaga aggggatcct
     1321 cgcccccggg atgctggccg atctggtggt cctcagccgc gacatcttcc atctcgagcc
     1381 gcgcgacctg cacgaggcgc gcgtcacgac gacgatcgtc ggcggccggg tcgtgtacga
     1441 agctccggct cagtgaccgc cgcacacctc ggcgcaggtg acgaccgcgg cgccgcgggc
     1501 gcacagcgtg tcggccgagc agccggattt ctggcagagc tggccggagc acttcggcag
     1561 cttgtcggcg gtctccgccg gcttcagggc gacgatcgcg ccgacgacca gggtgagggt
     1621 gcacgccgcg aggagcagtc tcttcttcat ggtggggtct ccttccgggg atgccgcgga
     1681 gtctaagagc ctgtccgaga cttggcaagg gccgcctata atgccgttcc gatgccctcc
     1741 accgcccggc actcgctgac cgagaagaat cgcgagatcc gcgccaactt cgacgccatc
     1801 gccgggcgct acgacctcac caaccgggtg atctcgtgcg gcgtcgacct ccactggcgc
     1861 aaggtggcgg tgcggacctt ttccgatctg ccgcgcggcg gccgggtgct cgatctcgcc
     1921 tgcggcacct gcgacctgtc gctggagctc ctgcgccgcc gtccggacgc gcgcgtcacc
     1981 ggggtggacc tgagccggac gatgttgtcg ctggcgcgtc cgaagatcgc cgcccgcgcg
     2041 gcttcggaca ggccgggcgg cgtgggcggc tttccgctgg tgaacgcccc ggccgaggcg
     2101 ctgccgttcg ccgacggcgt cttcgaaggg gcgatgatcg gcttcggcat ccgcaacgtg
     2161 cccgattacc gcgccgggct cggggagctg cggcgggtgc tgcgatccgg cgggcggctg
     2221 gtggtgctgg agttctcgac gccgccgtcg cggctcctct ggcgcgccta caacttctac
     2281 ttcttcaacg tcctcccccg catcggcgga atcctcaccg ggcgtgagcg ggcctaccgg
     2341 tacctgacgc actcggtggc ggagttcccc ggcgccgccg acttcggccg cgccatggag
     2401 caggccgggt tcgagcgggt cacgtggcgc tcgctcaccg gcgggatcgt ctgcgtgcac
     2461 accgggctcg ccgcgtgaag aggacataca ccgccgcccg gatcgaaggg ctgaagcagt
     2521 cggagatccg gcgcatgtcg ctcgagtgcg cgaaggtctc cggcatcaac ctcggccagg
     2581 ggatctgcga tcagccgatc gagccgatca tcaaggaggc ggccatccgc gccatcgacg
     2641 cggaccactc gatctacacg cgtctcgacg gcatcgacga gctgcgccgc cgcatcgccg
     2701 cgaagatgcg cggctacaac ggcatcgtct gcgaccccga cggcgaagtg gtggtgacgg
     2761 tcggcgccac cggggcgttc gtgaccgcct gcatggcgtt cctcgacccg ggggacgagg
     2821 tggtgatctt ttccccgttc tactcgtacc atgtcaacat cctgaaggtg tgcggggtgg
     2881 tgctgcgctt cgtgaccctg cgtccgcccg actggtcgta cgacccgcag gcgctcgaag
     2941 cggccttctc cgaccgcacc aagatggtcg tcctcaacac tccggcgaac cccagcggca
     3001 aggtgttcgg gaaggaggag ctcgaccacc tcgcggcgct gtgccgcaag cacgacgccc
     3061 tctgcgtcac cgacgaaatc tacgagtaca tcctgtacga cggccgccgc cacctgagcc
     3121 tcggcgcctt ccccggcatg gaggaccgca ccatcaccat cagcggcttc tccaagacgt
     3181 acgccatgac cggctggcgc ctcggctacg tcgtggcgcg ccgggcgctc gcctcgcaga
     3241 tggcgctcct caacgacttg atcaacgtct gcgccccgtc gccgctgcag cacggtgtcg
     3301 tcgcggcctt cgacctgccg ctggagtact acgaccggat ggtcgtggac tacacgcgca
     3361 agcgcgacat gaccgcggag gcgtgccggg ccgccggcat ggagccgttc gtgccgcagg
     3421 ggtcgtacta catgctcgcc gacgtgtccc gcctgggcat gaaggacgac tacgacgcgg
     3481 cgcgcatcct gctggagcag gccggcatcg ccgtcatccc cggctcgtcc ttctacgcga
     3541 acccggagga cggccgcatg cagatccgcc tctgctacgc gaagaagatg cccgacctcc
     3601 aggaggcctg ccggcgcctg cgcgccttca agccggtccc ggtctgacgt tctggtggta
     3661 ggcttaccgc cgctgaggag agcgtccatc atggcgaatc tctggattcg taagccgctc
     3721 gacaagctgg tcgccgaagc gggcgattcc gaaaagggat tgaagcgcac cctggggccg
     3781 ctcaatctca tcgccctcgg gatcggcgcc atcatcgggg cggggctctt cgtccgcacc
     3841 gccgcggcga tcgccgagcg ctccgggccg tcggtcacgc tcggcttcat cgtcgcaggc
     3901 atcggctgcg ccttcgccgg cctgtgctac gccgagttcg cgtcgatgat cccggtcgcg
     3961 gggagcgcct acacctactc ctacgccacc atgggggagt tcgtcgcctg ggtcatcggg
     4021 tgggacctgg tgctcgaata cgcggtcggc gccgccaccg tgtcgatcgc ctggagcgag
     4081 tatctcaaca agctcttcca gctgttcggg ttctccatcc cctaccag
//
LOCUS       DASRIQ010000403         5829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_696857, whole genome shotgun sequence.
ACCESSION   DASRIQ010000403 DASRIQ010000000
VERSION     DASRIQ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696857"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFD06_09870"
     CDS             <1..1120
                     /locus_tag="VFD06_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN03878.1"
                     /translation="AYDFIEKPPAAERLLLAVNNALRFERLREENRRLADDAGLAGRL
                     HGTSPAARELEATIGRVAPTDATVLILGENGSGKEIVARALHAASARRQRPLVTVNCA
                     ALPETLFESELFGHARGAFTGAVDARRGRFQSADGGTLFLDEIGEVPPALQPKLLRAL
                     ENGEVERIGGRGPEKIDVRLLAATNRDLEAEVRAGRFRQDLYYRLLVVPVRVPPLRDR
                     LEDVAALAVHFIETACRRNHVRAKRLSAEAIERLKGHSWPGNVRELRNAMERVAILAP
                     GDVIGSDAFDFLVPAAEGSARGSRDGGDRSATRASGAAAGETPDLAAEMERHEREMIV
                     KTLERHRWQMTKAAKELGLERSHLYKKLKALRIDKPED"
     gene            1314..2243
                     /locus_tag="VFD06_09875"
     CDS             1314..2243
                     /locus_tag="VFD06_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03879.1"
                     /translation="MTLRSTKESAARGAVALVLGLGLAAAPLTGCTEKKPSVSEMKNP
                     PNPPSPTPTPTETQVTLVTESTPPIDPKTAWKDGVAAYQSGEFSTSAKHLRVAVEEKK
                     DDPYAHYLFGLALWKSGQLDEAEGALVRSASMNEGSVKTWINLARVRLDRREFDGALE
                     AADRAILLDGSSSDALHQRGRALDGLNRGEEAVTTLEQARGLAPDNGYIANTLGYLLL
                     RRGRTDQAVAHLEAARDRLPQVAYVRNNLGVAYERSGDVHRAAEEFRAALQAGDSDGK
                     ASASLARIEPLLRERIASQPVPEEAGTEDQPQN"
     gene            2442..3167
                     /locus_tag="VFD06_09880"
     CDS             2442..3167
                     /locus_tag="VFD06_09880"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03880.1"
                     /translation="MNPEPMFSSSRRSTVVAAVLSLTLLAPAAAIFAAGSGPSSAPSG
                     SGMTQTGPTMTPAQQAAMRYNHGLKLQKKGDDKEKQAASASDEKKRAKAMESAQKAWG
                     KARQDYEQAVSLDPKNHQAHGALGYVLRKQGNFDASLASYERALQIKPGFTPAIEYRA
                     EAYLGLNRIEEAKADYITLFATDRANADTLAAAMTKWVEEKKKDPKGVEPATIDDLTK
                     WLESRQEIAAKTSALLPPKDARW"
     gene            3265..4323
                     /locus_tag="VFD06_09885"
     CDS             3265..4323
                     /locus_tag="VFD06_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnH family di-heme enzyme"
                     /protein_id="HZN03881.1"
                     /translation="MASGASAPGAPDTPAWEWRLPPGFPEPRVPPDNPMSAAKVALGR
                     RLFHERRLSLNETQACADCHRQALAFTDGRARAVGSTGAAHRRSAPSLANVAYAASLT
                     WADPNVRRLEEQARIPLLGTDPVEMGFGGREAELEARLRVDAGYAAHFASAFPGEAEP
                     VTFDNLTRALAAFERTLVSGNSAYDRLVYQGKMDALSDAAWRGLRLFFSERTGCASCH
                     GGLLFSGGFDYDRLERPAAPRFVDNGLLEAAERKGPQAVDPGLARATGRRRDRGLFKT
                     PTLRNIAVTAPYMHDGRIATLEGVLDHYARPGSTHPVGGGMILEAGEREDLLTFLRSL
                     TDESFLADPALAAPAGDR"
     gene            complement(4334..>5829)
                     /locus_tag="VFD06_09890"
     CDS             complement(4334..>5829)
                     /locus_tag="VFD06_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN03882.1"
                     /translation="AEANATGEGTLRRTLGPISLVALGIGAIIGAGLFVRTAAAIAER
                     SGPSVTLGFIVAGIGCAFAGLCYAEFASMIPVAGSAYTYSYATMGEFVAWVIGWDLVL
                     EYAVGAATVSIAWSEYLNKLLELFGVSIPYQFCHSPFQSLRDASGNVVASGIINIPAL
                     IILSLLTWLLIRGTHESAFVNGIIVVTKVAIVLMVIVLGWGYINPANHAVYIPEATSF
                     TNAEGVTHHYGGLLGILGAAGVVFFAFIGFDAVSTAAQEAKNPKKDMPIGILGSLVVC
                     TILYILFSHVLSGLATVEDFRTHGREASVAYAISTYMPGYQWLANLVTVAILAGFSSV
                     ILVMLLGQSRVFYSMSRDGLVPPVFGEVHPRYRTPWKSNLIFLVVTGLFAAFVPGDIV
                     GEMTSIGTLFAFMLVCAGVWILRVREPNRQRAFKTPLVPLVPILGILVCGAMIFGLGW
                     TNWLRLIAWLAIGLFIYFGYSRSHSLLGRQSPSTAGGRAPAPGHAET"
BASE COUNT          933 a         2083 c         2070 g          743 t
ORIGIN      
        1 agcgtacgac ttcatcgaga agccgccggc cgccgagcgg ctgctgctgg cggtgaacaa
       61 cgccctgcgc ttcgagcggc tgcgcgagga gaaccgccgc ctcgcggacg acgccgggct
      121 cgccggccgc ctgcacggga cctcccccgc ggcgcgcgag ctcgaggcga ccatcggccg
      181 ggtggcgccg accgacgcca ccgtcctcat cctcggggag aacggctccg gcaaggagat
      241 cgtcgcgcgc gccctgcacg ccgcgagcgc ccggaggcag cggccgctcg tcacggtgaa
      301 ctgcgccgcc cttcccgaga cgctgttcga gagcgagctg ttcgggcacg cgcgcggcgc
      361 cttcaccggc gcggtcgacg cccgccgcgg ccggttccag tcggcggacg gcgggacgct
      421 cttcctcgac gagatcggcg aggtgccgcc ggcgctgcag ccgaagctcc tgcgcgccct
      481 tgagaacggg gaggtggagc ggatcggcgg tcgcggcccg gagaagatcg acgtccggct
      541 gctcgccgcc acgaaccgcg atctcgaggc ggaggtgcgg gccggccgct tccgccagga
      601 cctctactac cggctgctgg tggtgccggt gcgcgtcccg ccgctgcgcg accggctcga
      661 ggacgttgcc gccctcgcgg tccacttcat cgagaccgcc tgccgccgca accacgtgcg
      721 ggccaagcgc ctgagcgccg aagcgatcga acgcctgaaa gggcactcct ggccggggaa
      781 cgtccgcgag ctgcgcaacg ccatggagcg ggtggcgatc ctcgccccgg gcgacgtgat
      841 cggcagcgac gcgttcgatt tcctggtccc ggcggcggag gggtcggcac gcggaagccg
      901 tgacggcggt gatcggagcg cgacgcgggc ttcaggagcc gcggccggag agacgccgga
      961 tctcgccgcc gagatggagc gccacgagcg ggagatgatc gtgaagaccc tcgagcgcca
     1021 tcgctggcag atgacgaagg cggcgaagga gctcgggctc gagcgcagtc acctctacaa
     1081 gaagctcaag gccctccgga tcgacaagcc cgaagactga gcctgcgtgt cggttcgcac
     1141 acgaagcggg ccgcggcggc ccgcgccgtt ccttcccgtc acggccctcc cgtcgaaaat
     1201 cgcccgtccc gggcgcgttt ccggcccgcc gcccatggca cgtcgctcgc ttttccgctt
     1261 ctccgagccc ggccaccccg acgaagggat tctccccggg ccaggagaga gccatgaccc
     1321 tgcgaagcac gaaagagtcc gccgcgcgcg gtgccgtcgc cctggtcctt ggactgggac
     1381 tcgccgccgc ccccctcacc ggatgcacgg agaagaagcc ctccgtctcc gagatgaaga
     1441 acccgccgaa cccgccgtcc ccgaccccga ccccgacgga gacgcaggtc actctggtga
     1501 ccgaatcgac tcctccgatc gacccgaaga ccgcctggaa ggacggcgtc gcggcgtatc
     1561 agtcggggga gttctccacc tccgcaaagc acctgcgcgt cgcggtcgag gagaagaagg
     1621 atgacccgta cgcccactac ctcttcggcc tcgccctctg gaagtcgggc cagctcgacg
     1681 aggcggaagg ggcgctcgtc cgctccgcct cgatgaacga aggctcggtg aagacctgga
     1741 tcaacctggc gcgcgtccgc ctcgatcgcc gcgagttcga cggcgccctc gaggcggccg
     1801 atcgggcgat cctcctcgac ggcagctcgt ccgacgcact gcaccagcgc ggccgggcgc
     1861 tcgacggcct gaaccgcggc gaggaggcgg tcacgaccct cgagcaggcg cgcggcctgg
     1921 ccccggacaa cggctacatc gccaacaccc tcgggtacct cctcctgcgc cgcggcagga
     1981 cggatcaggc ggtggcccat ctcgaagccg cgcgcgatcg gctgccccag gtcgcgtacg
     2041 tgcgcaacaa cctcggcgtc gcctacgagc ggagcgggga cgtgcaccgc gccgccgagg
     2101 agttccgcgc cgccctgcag gccggcgact cggacggcaa ggccagcgcg agcctcgccc
     2161 gcatcgagcc gctgctgcgg gagcgcatcg cctcgcagcc ggtccccgaa gaggccggca
     2221 cggaggacca gccgcagaac tgaccctgcc tcccccgatc gcccggcggg ggaagcctcg
     2281 ggaaccatcc cgccccgagg acccgccggg aacgagccgg ccgcccgctt catcacgaag
     2341 cgggcggccg gtgcgctttt cggcgcccct cgggcgtgct aggctccggg caggcgcggc
     2401 ggccccgaag ggagcgcgcg gcgcctccct ggaggccgaa cgtgaaccct gagccgatgt
     2461 tttcctcgtc gcgtcgttcc accgtcgtcg cagccgtcct ctccctgacg cttctcgcgc
     2521 cggccgcggc gatcttcgcc gccggcagcg gcccctccag cgcaccgagc ggcagcggca
     2581 tgacccagac cggtccgacc atgacgccgg cgcagcaggc ggcgatgcgc tacaaccacg
     2641 ggctgaagct gcagaaaaag ggggacgaca aggagaagca ggcggcctcc gccagcgacg
     2701 agaagaagcg cgccaaggcg atggagtcgg cgcagaaggc ctgggggaag gcgcggcagg
     2761 actacgagca ggcggtgtcg ctcgacccga agaaccacca ggcgcacggc gccctcggct
     2821 acgtgctgcg caagcagggg aacttcgacg cctcgctcgc gtcctacgag agggcgctgc
     2881 agatcaagcc gggattcacg ccggcgatcg agtatcgcgc cgaggcgtat ctgggtctca
     2941 accggatcga ggaggcgaag gcggactaca tcacgctgtt cgccaccgat cgcgccaacg
     3001 ccgacaccct cgccgccgcc atgacgaagt gggtggagga gaaaaagaag gacccgaagg
     3061 gggtcgagcc ggccaccatc gacgacctga cgaagtggct cgagtcccgc caggagatcg
     3121 ccgcgaagac gagcgccctg ctgccgccga aggatgcccg ctggtgaggg gttccgaccg
     3181 cttctgaccg tccgcgtccg ggcgttcgct cgattcgcgg ccggggccgc cgcgctcggg
     3241 ttcgcggcgg cggcttcctc tccggtggcc tcgggcgcct cggccccggg cgccccggac
     3301 acgccggcgt gggaatggcg cctccctccg ggtttccccg agccgcgcgt tccgcctgac
     3361 aacccgatgt cggcggccaa ggtggcgctg gggcgccgtc tcttccacga acgccggctg
     3421 tctctcaacg agacgcaggc ctgcgccgac tgtcaccggc aggccctcgc cttcaccgac
     3481 ggacgcgccc gcgcggtggg atcgaccggc gccgcgcacc gccgcagcgc gccgtcactg
     3541 gccaacgtcg cctacgcggc ctctctgacc tgggccgatc cgaacgtgcg ccgcctcgag
     3601 gagcaggcgc gcatcccgct tctcgggacc gacccggtcg agatggggtt cggcggccgc
     3661 gaggcggagc tcgaggcgag gctccgcgtc gacgcggggt acgccgccca cttcgcttcc
     3721 gcgtttccgg gcgaggccga gccggtcacc ttcgacaacc tgacgcgcgc cctcgcggcc
     3781 ttcgagcgca ccctcgtctc ggggaactcc gcctacgatc ggctggtcta ccaggggaag
     3841 atggacgcgc tgtcggacgc cgcctggcgc gggctgcggc tgttcttctc ggagcggacc
     3901 ggctgcgcct cgtgtcacgg cgggctcctg ttctcgggcg ggttcgacta cgaccggctc
     3961 gagcgcccgg cggccccgcg cttcgtcgac aacgggcttc tcgaggcggc ggagcggaaa
     4021 gggccgcagg cggtcgatcc ggggctggcg cgcgcgaccg gcaggcgccg cgatcgcggc
     4081 ctcttcaaga cgccgaccct gcgcaacatc gccgtgaccg ccccgtacat gcacgacggg
     4141 cgcatcgcga cgctcgaagg ggtcctcgac cactatgccc gccccggatc gacgcatccg
     4201 gtcggtggcg gcatgatcct ggaggcgggc gagcgcgagg atctcctgac gttcctgcgc
     4261 agcctgaccg acgagtcctt cctcgccgat ccggcgctcg cggccccggc cggagaccgc
     4321 tgagaaacgg tctctaggtc tcggcgtggc cgggggccgg cgcgcggcct cccgcggtgg
     4381 acgggctctg gcggccgagc aggctgtggc tgcggctgta gccgaagtag atgaacagcc
     4441 cgatcgccag ccaggcgatg agccgcagcc agttggtcca gccgagcccg aagatcatgg
     4501 cgccgcagac gaggatcccg aggatcggca cgagcggcac gagcggcgtc ttgaaagcgc
     4561 gctgccggtt cggctcgcgc acccgcagga tccagacgcc ggcgcacacc agcatgaagg
     4621 cgaagagcgt cccgatgctg gtcatctcgc cgacgatgtc tccgggaacg aaggcggcga
     4681 acagcccggt caccacgagg aagatgaggt tcgacttcca cggcgtccgg tagcgcgggt
     4741 gcacctcgcc gaagaccggc ggcaccaggc cgtcgcggct catcgagtag aagacgcgcg
     4801 actggccgag cagcatgacg aggatcaccg acgagaagcc ggccaggatc gccaccgtga
     4861 ccaggttggc cagccactga tagcccggca tataggtgga gatggcgtac gcgaccgagg
     4921 cctcccgccc atgcgtccgg aagtcctcca ccgtcgccag cccgctcagc acgtgcgaga
     4981 acaggatgta gaggatggtg cagaccacca gggaaccaag gatcccgatc ggcatgtcct
     5041 tcttcgggtt cttggcttcc tgcgccgccg tcgagacggc gtcgaagccg atgaaggcaa
     5101 agaagacgac ccccgccgcc ccgaggatgc cgagcaggcc gccgtagtgg tgcgtcaccc
     5161 cctcggcgtt cgtgaaggag gtggcctccg ggatgtacac ggcgtggttg gccgggttga
     5221 tgtagcccca gccgaggacg atcaccatca ggacgatcgc gaccttggtg acgacgatga
     5281 tgccgttgac gaaggccgac tcgtgcgtgc cgcggatcag gagccaggtc agcaggctca
     5341 ggatgatcag cgccgggatg ttgatgatgc cggaggcgac gacgttgccg gaggcgtcgc
     5401 gcagcgactg gaacggcgag tggcagaact ggtaggggat ggagacgccg aacagctcga
     5461 ggagcttgtt gaggtactcg ctccaggcga tcgacacggt ggcggcgccg acggcgtatt
     5521 cgagcaccag atcccagccg atcacccagg cgacgaactc ccccatggtg gcgtacgagt
     5581 aggtgtaggc gctccccgcc accgggatca tcgaggcgaa ttcggcgtag cagagcccgg
     5641 cgaaggcgca gccgatgccg gcgacgatga acccgagggt gaccgagggc ccggagcgct
     5701 cggcgatcgc cgcggcggtg cggacgaaca gcccggcgcc gatgatggcg ccgattccga
     5761 gcgcgacgag cgagatcggg cccagcgtcc gccgcagcgt gccttcgccg gtggcgttgg
     5821 cctcggcca
//
LOCUS       DASRIQ010000404         7365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2782695, whole genome shotgun sequence.
ACCESSION   DASRIQ010000404 DASRIQ010000000
VERSION     DASRIQ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2782695"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFD06_09895"
     CDS             <1..480
                     /locus_tag="VFD06_09895"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZN03883.1"
                     /translation="CWKRRFYPRLRALSYHRVREVQFTMANEPKTRKTRASVAAFLDR
                     IPDAGRREDCKAVAAMMRKASGRAPKMWGTGIVGFGSRRLKYASGKELDWPAVAFASR
                     KTDLTLYLGEFEGKAELLARLGKHKTSTACLYIKRLADVDRSVLETLILKTLEGTDC"
     gene            complement(511..1266)
                     /locus_tag="VFD06_09900"
     CDS             complement(511..1266)
                     /locus_tag="VFD06_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN03884.1"
                     /translation="MDTLNLLLILVAYAIVLLIALALGGHRSVSIASATATRDKPERI
                     LIIGATGRTGRHLLAQALDRGYAVTAFARDPSSIELRHPRLAIAKGDVLDRASVEAAM
                     PGHHAVISALGHRRYWSLSRTHTEGTRNVLWAMQRHGVRRLVCLTSLGIGDSAGRLGM
                     PYNLFVIPLVLPIYFWDKARQERVIAESDIEWIIVRPGSLNNGVKRERVNTGTRVGSY
                     LWTVRVSRANVADFMLNQLTSDTYLRSATGLAW"
     gene            complement(1298..2299)
                     /locus_tag="VFD06_09905"
     CDS             complement(1298..2299)
                     /locus_tag="VFD06_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03885.1"
                     /translation="MTPFPAVSLILLGVVVAAPVAEAAPARVDLPATVEVERFRMQSN
                     PWVNLHERLLYDAALGAPDPAGLTTEETAAWTAAVDAYRAWAKKRSPVFDAELIAVSQ
                     ALSGTRGRKLPDTIPEAARKVLAGAMPLYEKEQWPKDDTANRFWISVAQPLLKGCAGE
                     LVAAHEKAYGVPFTKRALVDASPFAGEFGGYTVGDAASVHTVISSTEPGYAGFAALEM
                     LMHEASHGIVAPDAGAIGADLARAAAETGRKPARNLWHALLFYTSGELTRRALAARGV
                     AEYRMAIEGRLFDGPFKGFREPFETHWRAWLDGALTRDEAIRRIVIATSPSGREGGS"
     gene            complement(2296..3846)
                     /locus_tag="VFD06_09910"
     CDS             complement(2296..3846)
                     /locus_tag="VFD06_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN03886.1"
                     /translation="MSANGVSRRDFLAAAGAGVAGAASVLESAVAAAKPAVPAASRTA
                     PKPDAAVPAPGSDPTGWTIREASQALRRGAVSSVDLTRACLERIDRLNPSLNACITVT
                     ADAALQAARRCDAERRAGKPHGALHGIPVALKDNIDTAGIRTTAASALYEDRIPAEDA
                     HVVRRLREAGAVLLGKLNLHEFAFGGTSTVSRFGPVRNPWALDRIAGGSSGGSAVAVA
                     AGLCFAALGTDTAGSVRIPASFCGVVGLKPTYGRVSNRGVIPLSWTHDHVGPLTRSVE
                     DAALVLQAIAGFDVADPASREVPAEPSLREIERTAAKPRLGIPRKPFFDDLDPEVAQA
                     VAAAIETLGRQAAGVVDVELPPGGFRSSGLYVRVRGPEAYAFHARTLKESPEKYQDPT
                     RAALMKFADVKADDYVNARREVELLRRTILETFSRVDLLVTPTMPVPPILIAEADREN
                     PVDWRNTVPFSTYGLPAVSIPCGRTRAGLPIGLQIAGPPLGEAAVLRFALAFERTAGR
                     NTMPRPAA"
     gene            complement(3954..6659)
                     /locus_tag="VFD06_09915"
     CDS             complement(3954..6659)
                     /locus_tag="VFD06_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN03887.1"
                     /translation="MSLRHRITTYFRNLRRGPAADREIDDEIDATLDLMADERERAGL
                     TPDEARRAARLDLAGGAAPGDSSGAPFGVEAVKESVRDARPGAALDGWLADIRQALRG
                     MARTPAFSAIVVLTLGVGIGAAMAIFGVVEATLLRPLPYPGSDRLALIWAQFGSESKA
                     PASGHQFLALRERSALFEAIGGIWVRSAALTGHGEPEQIRVGFVTADFLPLLVERPAL
                     GRHFTADEVGPGQEPLVILANELWRRRIGADEAIVGRTIQMDGQAVEVRGVLPERFRV
                     AFPEASRIPPDIDAFMPMPFDLAGGPLDVSFIRMLGRMRPGTTERQAQAEVDGIAAGL
                     RAEYKDYASQELQFRVAPLHAEVVHTARPAILALSGGVALVLLIACINVASLLLARGA
                     ERQRELTMRSALGAAPARLVRLLLTESVCLAAAGGGLALVLGWWGLAALQSLQPEGVA
                     RAGPTQVNLAVFGFALAITLAAGILSGLAPAVTATRGRIADALKRGGRGQAGGGAHRA
                     RNVLIGCEVALGCAVLASAGLMARTLHSLLRVDPGFDARGTLTLQVSLPGPRYPSDHD
                     RVDFARRIQEKLAALPGVESAGMGSNLPLDNSLPNWYAPYWLEDTPEDERNTMADHRS
                     VLPGFFRSIGARLLEGRDFEPVEIEQGRLVAIVDDSLARRTWPGRSAVGQRMALETMI
                     EGEFKWQVAEVIGVVRHVQFHSLVDEVREQVYIPYSLASRQQISFALRTSGDPESLAA
                     PARAALAALDADLPAARVVPLGHYVERARRATRFLSLLSASLAAIALLLAVVGVYGVS
                     SGSVTRRTSEIGVRVALGAKSGDVLRLVLSQGMKPVALGCVAGLALSVALAPLYSHLL
                     YGVRPFDPATYVLVTALIAAAGLLACWVPARRAVRLDPLEALRAE"
     gene            complement(6685..7050)
                     /locus_tag="VFD06_09920"
     CDS             complement(6685..7050)
                     /locus_tag="VFD06_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN03888.1"
                     /translation="MAARQDPEGAKSGDGKSDLVRGTLEMLILKALIPGPLHGLGVAR
                     RIAQITRGAFDVGPGSLFPALHRMEGEAWLTSAWSESENRRRARFYTLTKSGRRQLER
                     ESERWGRVSLAIARALEAT"
     gene            complement(7126..>7365)
                     /locus_tag="VFD06_09925"
     CDS             complement(7126..>7365)
                     /locus_tag="VFD06_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03889.1"
                     /translation="ERLLGAPHGRLRVPTNLLYDWFGPMKVHRAPPEATQAIVYCFMD
                     LAVQQDAAARRVKLLVLFGDADGTIAAVRRFDGTR"
BASE COUNT         1099 a         2534 c         2776 g          956 t
ORIGIN      
        1 tgctggaagc ggagattcta tccccgtctg cgcgccctgt cgtaccatcg cgtgcgggag
       61 gtgcagttca ccatggccaa cgaaccgaaa acgaggaaga ccagggccag cgtcgccgcc
      121 ttccttgaca ggattccgga cgccgggcgt cgcgaggact gcaaagccgt cgccgccatg
      181 atgcgcaaag cctcggggag ggcgccgaag atgtggggga cgggcatcgt cggcttcggc
      241 agccgccgcc tcaaatacgc cagcggcaag gagctcgact ggccggcggt cgccttcgca
      301 tcgcgcaaga cggacctgac cttgtatctc ggcgagttcg aggggaaggc cgaactcctg
      361 gcgcggctcg ggaagcacaa gaccagcacg gcgtgcctct acatcaagcg gctcgccgac
      421 gtggatcgaa gcgtcctcga aacgctcatc ctgaagacgc tggaggggac cgactgctga
      481 ccgtcgcgac gctccgggcg cggggcgtga ctaccaggcg aggccggtgg cggatcgcag
      541 gtaggtgtcc gaggtcagct ggttcagcat gaagtcggcg acgttggcgc gcgacacgcg
      601 caccgtccag aggtaggagc cgacgcgcgt cccggtgttg acgcgctcgc gcttgacgcc
      661 gttgttgagc gagccggggc ggacgataat ccactcgatg tcgctctccg cgatgacccg
      721 ctcctggcgc gccttgtccc agaagtagat cggcaggacc agagggatga cgaacaggtt
      781 gtacggcatc ccgagccgcc cggcgctgtc gccgattccg agcgaggtca gacagacgag
      841 acggcgcacg ccgtgccgct gcatcgccca caggacgttc cgggtcccct cggtgtgcgt
      901 gcggctcaag gaccagtagc gccggtgccc gagcgccgag atcaccgcgt ggtgacccgg
      961 catggccgcc tcgaccgagg cgcgatcgag gacgtccccc ttggcgatgg cgaggcgcgg
     1021 atggcgcagc tcgatcgacg acgggtcgcg cgcgaaggcg gtgaccgcgt aaccgcgatc
     1081 gagagcctgg gcgagcaggt gccgcccggt gcgcccggtc gcgccgatga tgaggatccg
     1141 ctccggcttg tcgcgcgtcg cggtggcgga ggcgatcgag accgaccggt gcccgcccag
     1201 ggccagcgcg atcagcagga cgatcgcgta cgccacgaga atcaggagca ggttcagcgt
     1261 gtccaaaccg gtccgcccat gagtccgtaa tgatccctca cgatccgccc tcccggccac
     1321 tcggggaagt cgcgatcacg atacggcgga tcgcttcgtc gcgcgtcagg gcgccgtcga
     1381 gccaggcgcg ccagtgggtc tcgaacggct cccggaaccc cttgaagggg ccgtcgaaca
     1441 gccgcccctc gatcgccatc cggtactccg cgactccgcg ggcggcgagg gcccggcggg
     1501 tcagctcgcc ggacgtgtag aagagcagcg cgtgccagag gttgcgcgcg ggcttgcggc
     1561 cggtctccgc cgcggcgcgg gcgaggtcgg cgccgatcgc cccggcgtcc ggcgcgacga
     1621 tgccgtgcga cgcctcgtgc atcagcatct cgagcgccgc gaacccggcg tagcccggct
     1681 cggtgctgga gatgaccgtg tgcaccgacg ccgcgtcgcc gacggtgtag ccgccgaact
     1741 cccccgcgaa gggcgaagcg tccacgagcg cccgcttcgt gaacggcacg ccgtaggcct
     1801 tctcatgcgc cgcgacgagc tcgccggcgc atcctttgag aagcggctgc gccacggaga
     1861 tccagaagcg gttcgccgtg tcgtctttgg gccattgctc cttctcgtag agcggcatcg
     1921 ccccggcgag gaccttgcgg gcggcctcgg ggatcgtgtc ggggagcttg cggccgcgcg
     1981 tcccggagag ggcctggctc acggcaatga gctcggcgtc gaagacgggc gagcgcttct
     2041 tcgcccaggc gcggtaggca tccacggcag cggtccacgc ggcggtctcc tcggtcgtga
     2101 ggccggccgg gtccggcgcc ccgagggcgg cgtcgtagag gagccgctcg tgcaggttca
     2161 cccaggggtt gctctgcatc cggaagcgct cgacctcgac ggtcgccggc aggtccacgc
     2221 gtgccggagc cgcctcggcg acgggagccg caacaacgac gccgaggagg atcagcgaaa
     2281 ccgcggggaa aggcgtcatg cggcggggcg gggcatcgtg ttgcgtccgg ccgtccgctc
     2341 aaaggccagg gcgaacctca agactgccgc ctctcccagg ggcggcccgg cgatctgcag
     2401 gccgatggga aggccggcgc gcgtccggcc gcatgggatc gacacggcgg gaaggccgta
     2461 ggtactgaat gggacggtgt tgcgccagtc gaccgggttc tcccggtcgg cctcggcgat
     2521 gaggatgggc ggcaccggca tggtgggcgt caccaggaga tcaacgcgcg agaaggtctc
     2581 gaggatcgtg cggcgcagca gctccacctc gcggcgagcg ttgacgtaat cgtcggcctt
     2641 cacgtcggcg aacttcatga gcgccgcgcg cgtcggatcc tgatacttct ccggagactc
     2701 cttgagggtg cgggcgtgga aggcgtacgc ctccgggccc cgcaccctca cgtacaggcc
     2761 gctcgaccgg aaccctcccg gcggtaactc cacatcgacc acgccggccg cttgcctgcc
     2821 gagggtctcg atcgcggcgg cgaccgcctg cgcgacctcg gggtcgaggt cgtcgaagaa
     2881 cggtttgcgc gggatcccca gccgcggctt cgcggcggtc cgctcgatct cgcgcaggga
     2941 cggctcggcc ggcacctcgc gcgacgccgg gtcggcgaca tcgaagccgg cgatcgcctg
     3001 cagcacgagc gcggcgtcct ccaccgaccg cgtgagcggc ccgacgtgat cgtgcgtcca
     3061 cgacagcggg atgacgccgc ggttgctcac ccggccgtag gtcggcttca gcccgacgac
     3121 gccgcagaac gaggccggga tgcggaccga gccggcggtg tcggtgccga gcgcggcgaa
     3181 gcagaggccg gctgccaccg cgaccgccga tccgccggac gaaccgccgg cgatgcgatc
     3241 gagggcccag gggttgcgga ccggtccgaa gcggctgacg gtcgacgtgc cgccgaaggc
     3301 gaactcgtgc aggttcagct tgccgaggag gacggcgccg gcctcccgga ggcggcgcac
     3361 gacgtgcgca tcctcggcgg ggatccggtc ctcatagagg gcgctcgccg cggtcgtgcg
     3421 gatccccgcc gtgtcgatgt tgtccttgag agcgaccggg atgccgtgga gcgctccatg
     3481 cggcttgccg gcgcggcgct cggcgtcgca ccggcgcgcg gcctgaagcg ccgcgtcggc
     3541 ggtgacggtg atgcaggcgt tcagcgacgg gttgagccgg tcgatccgct cgaggcaggc
     3601 ccgcgtgaga tcgaccgacg agaccgcgcc gcggcgcagg gcttgggagg cttcgcggat
     3661 cgtccacccg gtcgggtcgg agcccggagc cggaaccgcg gcgtccggtt tcggcgccgt
     3721 gcgggatgcc gcggggacag ccggcttcgc ggccgcgacg gccgactcga ggaccgacgc
     3781 cgctccggcg accccggcgc cggcggcggc gaggaaatcg cggcggctga ctccgttggc
     3841 gctcatcgcg cggagagtgt acccctgacg cggtcgaaga cggtgtcgct ggagttcggc
     3901 tgtcggctgc tcggcgttga ctacgacagg ggttggcgat tcggttctga gcgctactcg
     3961 gcccgcagcg cctcgagagg atcgagacgc accgcccggc gggccggcac ccagcaggcg
     4021 agcagcccgg ccgcggcgat gagcgccgtg accaggacgt aggtcgccgg gtcgaagggg
     4081 cgcacgccgt agagcaggtg cgagtagagc ggggccaggg cgaccgacag cgccagcccc
     4141 gcgacacagc cgagcgccac cggcttcatc ccctgcgaca gcacgagacg caggacgtcg
     4201 ccgctcttcg caccgagggc gacgcgcacg ccaatctccg aggtgcggcg ggtgacgctg
     4261 ccggaggaga cgccgtacac cccgacgacg gcgagcagca gcgcgatcgc cgcgagcgag
     4321 gcggagagca ggctcaggaa gcgcgtggcg cggcgagccc gctcgacgta gtgcccgagc
     4381 ggcaccaccc gcgccgccgg caggtcggcg tcgagcgcgg cgagggcggc gcgcgccggg
     4441 gcggcgagcg actccgggtc gccgctcgtc ctgagggcga aggagatctg ctgccgcgac
     4501 gcgagcgaat acgggatgta cacctgctcg cgcacctcgt ccaccaggga gtggaactgc
     4561 acgtgccgca cgacgccgat cacctcggcg acctgccact tgaactcccc ttcgatcatc
     4621 gtctcgagcg ccatccgctg tccgaccgcg ctccggccgg gccaggtgcg gcgcgccagc
     4681 gagtcgtcca cgatcgcgac gaggcgcccc tgctcgatct ccaccggctc gaagtcgcgc
     4741 ccttcgagca ggcgcgcgcc gatggagcgg aagaagccgg gcaggacgct gcggtgatcg
     4801 gccatcgtgt tgcgctcgtc ttcaggggtg tcctcgagcc agtagggcgc gtaccagttc
     4861 ggcaggctgt tgtcgagcgg caggttcgag cccataccgg ccgactcgac gccgggaagc
     4921 gcggcgagct tctcctggat gcggcgcgcg aagtcgacac ggtcgtggtc gctcgggtag
     4981 cgcgggccgg ggagggagac ctggagcgtc agggtgccgc gcgcgtcgaa gccggggtcc
     5041 acgcgcagga gggaatgaag cgtccgggcc atgagaccgg cgctcgcgag cacggcgcag
     5101 ccgagcgcca cctcgcagcc gatcaggacg ttgcgggcgc ggtgcgcacc tccgccggcc
     5161 tgaccgcggc cgccgcgctt gagcgcgtcg gcgatccggc cgcgcgtcgc cgtcacggcc
     5221 ggagcgagcc ccgacaggat gccggcggcg agagtgatcg cgagggcgaa gccgaacacg
     5281 gccaggttga cctgcgtcgg gccggcgcgc gccaccccct ccggctgcag cgactggagc
     5341 gccgcgagcc cccaccagcc gagaaccagc gccaggccgc cgcccgcggc ggcgaggcag
     5401 acgctctcgg tcaggagcag acgcaccagc cgcgccgggg cggcgccgag ggcgcttctc
     5461 atggtgagct cgcgctgccg ctccgctccg cgcgccagga gcaggctcgc gacgttgatg
     5521 caggcgatga ggaggacgag cgccaccccg ccggacaggg cgaggatcgc cgggcgcgcg
     5581 gtgtgcacga cttcggcgtg cagcggcgcg acccggaact ggagctcctg cgaggcatag
     5641 tctttgtatt cggcgcggag accggcggcg atgccatcca cctccgcctg cgcctggcgc
     5701 tccgtcgtgc cgggacgcat gcgccccagc atgcggatga acgagacatc gagcggtccc
     5761 ccggccagat cgaagggcat cggcatgaag gcgtcgatgt cgggggggat gcggctcgcc
     5821 tcggggaacg cgacgcggaa gcgctccggc aggacaccgc gcacctccac cgcctggccg
     5881 tccatctgga tggtgcggcc gacgatcgcc tcatcagcgc cgatgcggcg acgccacaat
     5941 tcgttcgcga ggatcaccag cggctcttgt cccgggccca cctcgtcggc ggtgaaatgc
     6001 cggccgaggg ccgggcgctc gacgaggagc ggcaggaagt cggcggtgac gaaaccgacg
     6061 cggatctgtt ccggctcgcc gtggccggtg agggcggcgc tcctcaccca gatgccgccg
     6121 atcgcctcga agagcgcgct gcgctcgcgc agggcgagga actggtgacc ggaggcgggg
     6181 gctttcgact cggagccgaa ctgcgcccag atgagggcca gccggtcgga gcccgggtac
     6241 ggaagcggcc gcagcagggt cgcctcgacc acgccgaaga tcgccatggc ggcgccgatc
     6301 ccgaccccga gcgtcaggac gacgatggcg gagaaggcgg gcgtccgcgc catgccgcgg
     6361 agcgcctgcc ggatgtcggc gagccagccg tccagcgcgg cgccggggcg ggcgtcgcgg
     6421 acgctctcct tgaccgcctc gacgccgaac ggcgcgccgc tcgaatcgcc cggcgcggcc
     6481 ccgcccgcga ggtcgaggcg ggcggcgcgg cgggcttcgt ccggagtgag gccggcgcgc
     6541 tcacgctcgt cggccatgag atcgagggtg gcgtcgatct cgtcgtcaat ctcgcggtcg
     6601 gcggcggggc cgcgccggag gttccggaag taggtggtga tgcggtggcg gagcgacatg
     6661 ggcgtcgcgc tctcctctgt gacgctaggt cgcctcgagc gcgcgggcga tggcgagcga
     6721 cacgcggccc cagcgctccg actcgcgctc cagctgccgg cgccccgatt tcgtcagcgt
     6781 gtagaagcgg gcgcgccgcc ggttctccga ctcgctccag gcagaggtga gccacgcctc
     6841 cccctccatg cggtgcagtg cggggaagag ggagccgggg ccgacgtcga aggcgccgcg
     6901 ggtgatctgc gcgatgcgcc gcgccacgcc aaggccgtgc agcggcccgg ggatgagcgc
     6961 cttgaggatg agcatctcga gcgtcccgcg caccaggtcg gacttcccgt caccggactt
     7021 cgcgccttcg gggtcctgcc tggccgccac tacaccctcc tatcgagaat cgacaggaga
     7081 gtacgccgac tcctatcgat tgtcaatagg agagtgggtt ctcggtcagc gggtcccgtc
     7141 gaagcggcgg accgcggcga tcgtcccgtc ggcgtccccg aagaggacca gcagcttgac
     7201 gcggcgggcg gcggcgtcct gctggacggc gaggtccatg aagcagtaga cgatcgcctg
     7261 ggtcgcttcc ggcggcgcac ggtgcacctt catggggccg aaccagtcgt acaggaggtt
     7321 cgtcgggacc cgcagccggc cgtgcggcgc gccgagcagg cgctc
//
LOCUS       DASRIQ010000405         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_697058, whole genome shotgun sequence.
ACCESSION   DASRIQ010000405 DASRIQ010000000
VERSION     DASRIQ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697058"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VFD06_09930"
     CDS             <1..983
                     /locus_tag="VFD06_09930"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HZN03890.1"
                     /translation="RFEVSAWLNVPYLTVFLVGFVTFWIFGVSLRTLPVFMGLRDRPR
                     LAAALAAPLSIATAFTALSEGLFLRTGAPIAGVAFGTGLVALAILLAAFAVALGALVP
                     AREAPEPGVDRGYEKYVRLAYAWLVLAGLMLGAFSVVIFTGHGLDHGYVGAYRHALTV
                     GFITTLMVGMAQRIVPVFRGVPLHSARLLEWTFWLLAIGNLIRVLFQSLSAAFGPVWL
                     RVAGVSGLLELAALLLFAVNLWKTMNAPTADDRAAAAYRSPIAAEARVGDLLQAYPAL
                     LPVFVGSGFAALGNPVLRRTLARGVSVAQACRMHGVEVEGFLRRLRDSVT"
     gene            1033..1524
                     /locus_tag="VFD06_09935"
     CDS             1033..1524
                     /locus_tag="VFD06_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03891.1"
                     /translation="MLQHAGRLLSGLSSFALAWLLASSGANAQESDHYVLEGVTVTAG
                     AEAAASARFDLSVVVAALEPTGAASFCNDGFGVVLGGAPLAAPLPVPNHLLVQRNGAN
                     PGHVDLTWSGTAATFDVYRGTSPVNLATPGNYVTTSPGCLVIDTDPFTGPIVYYTVIP
                     AEN"
     gene            1561..>2953
                     /locus_tag="VFD06_09940"
     CDS             1561..>2953
                     /locus_tag="VFD06_09940"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZN03892.1"
                     /translation="MSRSMRSAAPFGALLLAALLLAPSAGPSAATPQTIKVQGSLTDR
                     VSDTPAPAQGVFTMTFALFDNEFAGLPITTIGPLQVDVAQGRFQVELPLSTAQFQVPD
                     RYLAITVNGETLTPRIRLTSAPFALVADQSATASSAGSAGVALSVAAGGVGTASLAPG
                     SVTADKLGIPCATGEILVRSGSAWVCAPQPQTGTICSPGSFVSCYSGPAGTLNVGACR
                     AGAAHCNAAGTAFEGCTGQVLPAAESCDNADNNCDGAVDESNICPPCPDADSDGFTDA
                     ACIGGNDCNDQSASVHPGAAELCNYGVDDDCNALTPDGLTDPQAGSPCDGADTDLCSE
                     GVQFCAGGSFVCTDNTGNIVEICNGLDDDCSGSVPQNEIDSDGDGYKPCNGDCNDANA
                     TIHPGAADLCDTIDQNCDGSNFGSDPRVGVPCDGSDADLCLEGTSSCSQFGAILCNDV
                     TAGTFDVCNGLDDD"
BASE COUNT          400 a         1072 c          993 g          488 t
ORIGIN      
        1 gccgattcga ggtgtcggcc tggctcaacg tgccttacct gacggtgttc ctcgtcgggt
       61 tcgtcacctt ctggatcttc ggcgtgtcgc tccggacgct gccggtcttc atgggactcc
      121 gcgatcgccc gcggctcgcc gcggctctgg cggcgccgct gtcgatcgcc accgccttca
      181 ccgcgctctc cgagggactg ttccttcgca ccggcgcacc gatcgccgga gtggccttcg
      241 ggacgggact cgtcgcgctg gcgatcctgc tggcggcctt cgcggtcgcg ctgggagcgc
      301 tcgttcccgc ccgcgaggcg ccggagccgg gagtcgatcg cggctacgag aaatacgttc
      361 gcctcgccta cgcctggctg gtgctggcgg ggctgatgct tggcgctttc tccgtcgtca
      421 tcttcacggg gcacgggctc gaccatgggt acgtcggggc ctaccgccat gcgctgaccg
      481 tcggcttcat cacgacgctg atggtcggca tggcgcagcg gatcgtgccg gtgttccgcg
      541 gcgtgccgct ccattccgcc cgcctgctcg aatggacctt ctggcttctg gcgatcggca
      601 acctcatccg cgtgctgttc caatccctct ccgccgcctt cggccccgtc tggctgcggg
      661 tcgcgggggt gtcgggactc ctggagctgg cggcgctcct cctgttcgcc gtgaacctct
      721 ggaagacgat gaacgcgccg accgcggacg accgggctgc cgccgcctac cggtcgccga
      781 tcgccgccga ggcccgcgtc ggcgaccttc tgcaagccta cccggcgctc ctgccggtct
      841 tcgtcggcag cggcttcgcg gcgctcggca atccggtgct gcggcggacg ctggcccggg
      901 gggtgagcgt ggcgcaggcc tgccggatgc acggcgtcga ggtggagggc ttcctccgcc
      961 ggctgcgcga ttccgtcact tgacagagga ggtggtccgt cgtacaccac ccccatccat
     1021 cggggtgagc cgatgctgca gcacgcggga cgtctgttgt cagggctctc gagcttcgcg
     1081 ctcgcgtggc tgctcgcctc ttccggcgca aacgcccagg aatcggacca ttacgtcctc
     1141 gaaggagtga cggtgaccgc cggggccgaa gcggcggcct ccgcgcgctt cgacctgtcg
     1201 gtggtcgtgg cggcgctcga gccgaccggc gccgcctcct tctgtaacga cgggttcggt
     1261 gtcgtcctgg ggggtgcgcc gctcgctgcg ccgctgccgg tgccgaacca tctcctggtg
     1321 cagcgcaatg gcgccaaccc cggccacgtc gatctgacct ggtccggaac cgcagcaact
     1381 ttcgacgtct accgcgggac gtcgccggtg aacctggcca cccccggcaa ttacgtgacc
     1441 acgtcgcccg gctgcctggt catcgatacc gatccgttca cgggaccgat cgtctattac
     1501 accgtcatcc cggcggagaa ctgagacacg gcggcccccg aggcgaccac gggaggtccg
     1561 atgtcacgct cgatgcgcag cgcagctccc ttcggggctc ttctcctcgc ggccctgctc
     1621 ctggctcctt ccgccgggcc gtcggccgcg accccccaga cgatcaaggt ccaggggtcg
     1681 ctcaccgatc gcgtctccga cacgcccgcc cccgcccagg gggtgttcac catgaccttc
     1741 gccctgttcg acaacgagtt cgccgggctg ccgatcacga ccatcggccc gctccaggtg
     1801 gacgtggcgc agggccgctt ccaggtggag ctgccgctga gcacggcgca gttccaggtc
     1861 ccggaccgct acctcgcgat caccgtgaac ggcgagacgc tgacgccgcg catccgcctc
     1921 acgagcgcgc cgttcgctct ggtggcggat cagtcggcga ccgcgtcctc cgccggctcc
     1981 gcgggcgtgg cgctcagcgt cgcggccggc ggtgtcggca ccgcctctct cgccccgggg
     2041 tcggtgaccg cggacaagct cggcatcccg tgcgccacgg gtgaaatcct cgtgcgcagc
     2101 ggatccgcgt gggtgtgcgc cccgcagccg cagaccggga cgatctgcag tccggggagt
     2161 ttcgtctcct gctacagcgg gccggccggc acgctgaacg tgggcgcgtg ccgcgccggc
     2221 gcggcccatt gcaacgccgc cgggaccgcg ttcgaaggct gcacgggaca ggtcctgccg
     2281 gccgccgagt cctgcgacaa cgcggacaac aactgcgatg gagccgtgga cgagtcgaac
     2341 atctgcccgc cctgtccgga cgcggacagc gacgggttca ccgacgcggc ctgcatcggc
     2401 ggcaacgatt gcaacgacca gagcgcttcg gtgcacccgg gggcggcgga gctctgcaat
     2461 tacggcgtcg acgacgactg caacgccctg actcccgacg gcctcaccga cccgcaggcc
     2521 ggatcgccct gtgacggagc ggacacggat ctctgcagcg agggggttca gttctgcgcc
     2581 ggcgggtctt tcgtctgcac cgacaacacg ggcaacatcg tggagatctg caacggcctc
     2641 gacgacgact gcagcgggag cgttccgcaa aacgagatcg acagtgacgg ggacggatac
     2701 aagccctgca acggcgactg caacgacgcc aacgccacca tccaccccgg agccgcggat
     2761 ctctgcgaca ccatcgacca gaactgcgac ggttccaact tcggcagcga cccgcgggtc
     2821 ggagttccct gcgacggctc cgacgcggac ctctgcctcg aggggacgag cagctgtagc
     2881 cagttcgggg ccatcctctg caacgacgtc acggcgggga cgttcgatgt ctgcaatggc
     2941 ctcgacgacg act
//
LOCUS       DASRIQ010000406         4228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3029581, whole genome shotgun sequence.
ACCESSION   DASRIQ010000406 DASRIQ010000000
VERSION     DASRIQ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3029581"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFD06_09945"
     CDS             complement(<1..183)
                     /locus_tag="VFD06_09945"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZN03893.1"
                     /translation="MKRDAVIVAGARTPMATYNTHFRDLPETELGALAARAALQRAQA
                     SPGNVDQVVFGNAMQTS"
     gene            complement(180..1019)
                     /locus_tag="VFD06_09950"
     CDS             complement(180..1019)
                     /locus_tag="VFD06_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN03894.1"
                     /translation="MDEPDSTAACAACGGTGFVLVERKGQPLARLCACRKRAASPLPA
                     EQALERARVPRRFWNCDFEHFFDHSIDLVGAKKMALRYAEEYPLEDQGLLLMGSPGVG
                     KTHLAVAILRRVATEKGVTGLFCDAQDLLRRLQATFERSSGRGEEEVLEPVLSADLLV
                     LDDLGGRLPTPWVEDTLLHIVTTRYNERRPMIITTNYFDAPPERGGARPSPSPAPASP
                     AKKDAETRAATLTDRIGPRMRSRLYEMCRAVVIVADDFRVRVKNEGHHWPSQNLGRPQ
                     GDA"
     gene            complement(1033..1905)
                     /locus_tag="VFD06_09955"
     CDS             complement(1033..1905)
                     /locus_tag="VFD06_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HZN03895.1"
                     /translation="MTLVDEDKVVRRPAAATSETLKKYLQEISRLPRITPEEEKILGD
                     KIQRGDKAALRKLVEANLRFVVSFAKRYRNCGLSFLDLINEGNIGLIEAAKRFNPKKN
                     VKFITYAVWWVRQAIIHALSDQSGAFRLPQKQANLLYRIGKTGQQLELELERKPTAEE
                     IAKKLDVPTEEVINLLQVNDENVSLSTVIDEEHEFHLSDKLEQDLIPSADAELMRSSL
                     KDHLYSCLGELEPKEQKVLRLRFGLDEGDPKTLKEIGEMMGLSRERIRQIEAQALEKL
                     HRSQKCQQLQGYLN"
     gene            complement(1902..2294)
                     /locus_tag="VFD06_09960"
     CDS             complement(1902..2294)
                     /locus_tag="VFD06_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN03896.1"
                     /translation="MRRRKDSAYYMISVVARQFEVHPQTLRLYEREGLLLPSRTDGNT
                     RLYSDKDLEVLSFILSLTRDLGVNLAGVEIILNMRRKMEAMQMEMDRFVALLREEVAR
                     RQGTGGGTSQALVRVGPKERGTVKVRKG"
     gene            complement(2308..3420)
                     /gene="dnaJ"
                     /locus_tag="VFD06_09965"
     CDS             complement(2308..3420)
                     /gene="dnaJ"
                     /locus_tag="VFD06_09965"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZN03897.1"
                     /translation="MTTHYDILGIERKARLGDVKRAYKRLARRHHPDLNPGDRRAEER
                     FKKISEAYAVLSDPTRRRAYDREIDAPPRAGVSSGPAPPAGWDGDAFTFESGDPGAFA
                     SFFSEFFHPQGSEARPRHAPRRGDDVTRTVAIGFFDALRGLVTEIEIDAETPCGQCQG
                     SGRVPAAQRRPCPDCAGTGRISRFPGPLRLTAVCPRCEGEGALSWDGCGSCAGAGVLT
                     RRERVEVRLPAGVDDGSRVRIPGKGRAGRNGGASGDLFLVTRVEPHPYFRRIGDNIHS
                     TVPITVSEAALGARLEVPTIDGRASMRIPPGTETGQRFRLRGKGAPSLRGTARGDHYV
                     ETRIATPRTTADRTRRLLEELGGLESGEELRRDLLK"
     gene            complement(3422..3913)
                     /locus_tag="VFD06_09970"
     CDS             complement(3422..3913)
                     /locus_tag="VFD06_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZN03898.1"
                     /translation="MIRRGGRGGRPVRGAAAPTAWDPLRELAGLKDRLNKLFESTLRH
                     GGSVDEAGMTGWAPACELREETGAYVLTAEVPGVPRGSLRLRLEAGSVVIEGERPQAP
                     ETRKGQPLRVERSYGRFVRSFPLPGGVDEGGVKARLEQGVLEVRLPKSDRGRTAAVPI
                     RVR"
     gene            complement(3910..>4228)
                     /gene="dnaK"
                     /locus_tag="VFD06_09975"
     CDS             complement(3910..>4228)
                     /gene="dnaK"
                     /locus_tag="VFD06_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZN03899.1"
                     /translation="LIYNTEKILRENRDKVSEADAAAVETSLGNAKRALDSNEASELN
                     SAFEDLTRSSHRLAEAMYQKTSGEQAPPTDAGRQGGEPKGGGKKDEEVIDAEYVDVDE
                     GKR"
BASE COUNT          559 a         1505 c         1452 g          712 t
ORIGIN      
        1 gctcgtctgc atggcattgc cgaagacgac ctgatcgacg ttgcccggcg aggcctgggc
       61 gcgctgcagc gccgcgcgcg cggcgagcgc ccccagctcg gtctcgggaa ggtcccggaa
      121 atgggtgttg taggtcgcca tcggcgtgcg ggcgccggcg acgatcacgg cgtcgcgctt
      181 cacgcgtccc cctggggccg gccgagattc tgcgacggcc agtgatgccc ctcgttcttg
      241 acccgcacgc ggaagtcgtc ggccacgatg acgaccgcgc ggcacatctc gtagagacgc
      301 gagcgcatgc gcgggccgat ccggtcggtc agcgtcgcgg cgcgcgtctc ggcgtccttc
      361 ttcgcggggg acgccggcgc gggagagggc gagggacggg cgccgccccg ctccggcggg
      421 gcgtcgaagt agttggtcgt gatgatcatc ggacgccgct cgttgtagcg ggtggtgacg
      481 atgtgcagga gcgtgtcctc gacccacggg gtggggagcc gccctccgag gtcgtcgagc
      541 accagaagat cggcgctcag caccggctcc aggacctcct cctccccgcg acccgaggag
      601 cgctcgaagg tggcctgcag gcggcgcagc agatcctggg cgtcgcagaa caggcccgtc
      661 acccccttct cggtggccac ccggcgcagg atcgcgaccg cgaggtgcgt cttgccgacg
      721 cccggcgacc ccatgaggag gagcccctga tcctcgagcg ggtactcctc ggcgtagcgc
      781 agggccatct tcttcgcccc gacgaggtcg atcgagtgat cgaagaagtg ctcgaagtcg
      841 cagttccaga accgccgggg gacgcgcgcc cgctcgagcg cctgctcggc cgggagcggc
      901 gacgcggccc gcttccggca ggcgcacagc cgcgccagcg gctgcccctt ccgctccacg
      961 agcacgaacc ccgttccgcc gcacgcggcg caggcggcgg tgctgtctgg ttcgtccatg
     1021 agactcccgg cgtcagttca ggtagccctg cagctgctgg cacttctgcg agcggtgcag
     1081 cttctccagg gcctgggcct cgatctggcg gatgcgctcg cgcgacagcc ccatcatctc
     1141 gccgatctcc ttgagggttt tcggatcccc ctcgtcgagc ccgaaccgga ggcgcagcac
     1201 cttctgctcc ttcggctcca gctcgccgag gcaggagtag aggtggtcct tgagcgagct
     1261 gcgcatcagc tcggcgtcgg ccgaggggat gaggtcctgt tccagcttgt cggacaggtg
     1321 gaactcgtgc tcctcgtcga tgacggtcga gagggacacg ttctcgtcgt tcacctgcag
     1381 gaggttgatc acctcctcgg tcggcacgtc gagcttcttg gcgatctcct ccgcggtcgg
     1441 tttccgctcc agctccagct cgagctgctg ccccgtcttg ccgatgcggt agaggaggtt
     1501 ggcctgcttc tgcgggagac ggaaggcgcc gctctggtcg gacagggcgt ggatgatcgc
     1561 ctggcgcacc caccagacgg cgtaggtgat gaacttgacg ttcttcttcg ggttgaagcg
     1621 cttcgccgcc tcgatcaggc cgatgttccc ctcgttgatg agatcgagga acgacaggcc
     1681 gcagttgcgg taccgcttcg cgaacgagac cacgaagcgg aggttggctt ccacgagctt
     1741 gcgcagcgct gccttgtcgc cgcgctggat cttgtccccg aggatcttct cctcctccgg
     1801 ggtgatgcgc ggcaggcggg agatctcctg gaggtacttc ttgagtgtct cggacgtcgc
     1861 ggcggccggc ctgcgcacga ccttgtcctc gtcgacgagg gtcatccctt gcgcaccttg
     1921 accgtaccgc gctctttcgg cccgacgcgc accagggcct ggctcgtccc tccccccgtc
     1981 ccctgccgcc gcgcgacctc ttcgcgcagc agggctacga agcgatccat ttccatctgc
     2041 atcgcctcca tcttgcgccg catgttgagg atgatttcga ccccggccag gttgacgccc
     2101 aggtcccggg tcaggctgag gatgaacgac aggacctcga ggtccttgtc ggaataaagc
     2161 cgcgtgttgc cgtcggtgcg cgacgggagc aggagcccct cgcgctcgta caggcgcagg
     2221 gtctgggggt ggacctcgaa ctgccgggcc accaccgaga tcatgtagta ggccgagtcc
     2281 ttgcgccggc gcatgctccg ctccgcctca cttcaagaga tcgcggcgga gttcctcccc
     2341 gctctcgagg ccgcccagct cctcgagcag ccgccgcgtc cgatcggccg tggtgcgcgg
     2401 cgtcgcgatc cgggtttcca catagtgatc gccgcgcgcc gtgccgcgca gggagggggc
     2461 tcccttgccg cgcagtcgga atctctggcc tgtctcggtg cccgggggga tgcgcatgct
     2521 ggcccggccg tcgatcgtcg gaacttcgag gcgcgcgccc aaggccgctt cggacaccgt
     2581 gatcggcacg gtgctgtgga tgttatcacc gatccttcgg aagtacggat gcggttcgac
     2641 gcgtgtgacg agaaagagat cgccggaggc gcccccgttg cggccggccc ggccctttcc
     2701 cgggatgcgg acgcgcgatc cgtcgtcgac gccggccggg agccgcacct cgacccgttc
     2761 gcgccgcgtc aggactcccg ccccggcgca cgagccgcag ccgtcccacg acagcgcccc
     2821 ctccccctcg cagcgcgggc agacggcggt gagccgcagc gggcccggga agcggctgat
     2881 gcggccggtc ccggcgcagt cgggacaggg gcgccgctgc gccgccggca cgcggccgga
     2941 gccctggcac tggccgcaag gcgtctcagc gtcgatctcg atctcggtga cgagcccacg
     3001 caacgcgtcg aagaacccga tcgccacggt gcgggtgacg tcgtcgccgc gccgcggcgc
     3061 gtgccgtggc cgcgcctcgg acccctgcgg gtggaagaac tcgctgaaga aagacgcgaa
     3121 ggcgcccggg tcgccgctct cgaaggtgaa ggcatcgccg tcccatccgg ccggcggcgc
     3181 cggtccgctc gagacaccgg ccctcggtgg ggcgtcgatc tcgcgatcgt aggcgcggcg
     3241 tctcgtggga tcgctgagga cggcgtacgc ctcgctgatc ttcttgaagc gctcctcggc
     3301 gcggcgatcc cccggattga ggtcgggatg gtggcgccgc gccaggcgct tgtaggcccg
     3361 cttgacgtcc cccaagcggg ccttgcgctc gatccccagg atgtcgtaat gcgtcgtcat
     3421 ctcaacggac gcggatcggc acggccgcgg tccggccgcg atccgatttc ggcagccgga
     3481 cctcgagcac cccctgttcg agccgcgcct tcacgccacc ctcgtccact cctccaggga
     3541 gcgggaagga ccttacgaat cggccgtacg accgctcgac gcgcagcggc tgccccttgc
     3601 gggtctccgg cgcctggggt cgctccccct cgatcaccac gcttccggct tcgagccgaa
     3661 gacggagact tccgcgcggc acgccgggga cctcggcggt caggacgtac gctccggtct
     3721 cctcccgcag ctcgcaggca ggcgcccagc cggtcatccc ggcttcgtcg acgctgccgc
     3781 cgtggcgcag ggtgctctcg aacagcttgt tgagccggtc cttgagaccc gccagctcgc
     3841 gcagagggtc ccacgccgtc ggcgccgcgg cgccgcggac cggacgacct ccgcggccgc
     3901 cgcggcggat caccgcttcc cctcgtccac gtcgacgtac tcggcgtcga tgacctcctc
     3961 gtccttcttg ccgccgccct tgggctcgcc gccctgacga ccggcgtcgg tcggaggagc
     4021 ctgctcgccc gaggtcttct ggtacatcgc ctcggccagt cgatgcgatg agcgggtcag
     4081 gtcctcgaac gcgctgttga gctccgatgc ctcgttgctg tcgagcgcgc gcttggcatt
     4141 gccgagtgag gtctcgaccg cggcggcatc cgcctcgctc accttgtccc ggttctcgcg
     4201 caggatcttc tcggtgttgt agattaag
//
LOCUS       DASRIQ010000407         6182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1220091, whole genome shotgun sequence.
ACCESSION   DASRIQ010000407 DASRIQ010000000
VERSION     DASRIQ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220091"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..3679
                     /locus_tag="VFD06_09980"
     CDS             125..3679
                     /locus_tag="VFD06_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HZN03900.1"
                     /translation="MTVTVRLLCIAAASMLWLGRAHAAAPGDAAAPGDRPVLRPGVET
                     TVRLEGKDGRAFTLKLGAGDRVGFRLVRHEGPIALVLEDPAARERRRWIRDPADGSDT
                     ICPRPRRPAAAWIADAAGSWTLRVLPPEGVPPFEVTLVRDPPRKATDMDRLAFAADLE
                     EARVEALLEEGKGAEAAEAAKALVEARSREESGEPLSLASDLGYLGVHFYQSASCRSG
                     AWDVAAGLVRQALELRAAALPAGDLCVAESLSLVASVLYAQGRFEEGETLEKRSLAIR
                     EVALPGARESWRNSLRDVGLLLLQQGKFAEAEAYLVRAREAAATTGGDAPHALADARH
                     NLGELYRNQNRYDAAAEELTSAEALERAQTDPDLGLLSDILAALAGVHLDRADYDEAE
                     RYAKLYLDLLAQHAEIASADHLAIGRVNLGEIYRRQGRLGEAEPLLVEALAAARETFC
                     PENPRLLYFRLDVALLYSEQGKHEAAEALYRESLLTLEEALGPRHPDVAANLQELAGM
                     LAGRGRFSEAVPLYERALAIREETLGADHPLTAASRTRLARARLDGGAGDFEGLLASA
                     VRARRALETSVSYPEEAVDAFSFEADLLDRLGRKREARQALAEALARVERLRPRRGGG
                     EMARVDFLRRHLDDYDRIVDWSLDAGDLDAAFAYAERSRARALLDLLTAGTGDLRGSM
                     APAVRSVLETRETEATSELAEAQGRAAYERARTDLDAAQRRARLADLERQIDAAARRL
                     QVVADDFKNNSPLWRDEVGAHGGPISLPEARRDVVPPHGVMLAYRVGRLASLVFVIPP
                     APAKVIVSRLVVSAGDATTLGIPAGPLTASALQSALTGDPAAPEIPLLARIAEPPGGP
                     TEDAAATIRELHALRRVLVPDVVRARMRRAQEVVVIPDGALALLPFEVLVFDPAPDWS
                     GARLWLDSGPTVRYAASATSLGSVIARAASRVQGRPTVLTVSDPIFDPAELDGASPPA
                     VPVDSSSGGRRLQRLPGTARESAAVEAAFREAGFVDVVTRLDGARATEAAVRMALPGK
                     RFVHLATHGIVKPSRSDLLAGLALTVPRGIPASAAEDGMLQLFEIEEQKLAAELVVLS
                     ACDTNVGRRIAGEGVFSLARGFFAAGAGSVVASLWPVDDSSSARLVGDLFRGIAGAAA
                     GRDAHPAEALRDAKRRLRAKPATAAPFFWAAFTCTGIR"
     gene            complement(3693..5822)
                     /locus_tag="VFD06_09985"
     CDS             complement(3693..5822)
                     /locus_tag="VFD06_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03901.1"
                     /translation="MRTATALRTCSRLGALSTALLFAATIPAVAQDVSQRLSSNLNGR
                     TYEPTYVFFTAQGSEAILNLSRVNAVCVSTMSCTTDTQCAASLPGTLCRGTPLRCQGP
                     VDALLGQAAVIRQSSFCELAACAGASIGTAGCAGVTCPPDQSYCGSPGSCHTCPQAEA
                     LCLCDIGGLPAACPESDAVFGPAVAAFNDGFDDGTNPNHWSLMESSIAPNPPDTRLVL
                     GTGAALPSGASIAAAGVRFAVQAGQTYVLFLKRAVDPRAAWVSTCFPSGDLSLFLASR
                     APLWCDQDDDGFDGTDCDPGNGQAWAIPGEPGDVAFSIAGGVTTLGWAQPVPPGGTPA
                     GIAYRVYAGSRADLPNLLNVKADSCLVADTPGLSAPIVADPAPGTLAWYLVRAADSCG
                     EGTAGNATAGPRIQDARSCDDTIACTIDSCDSSLGCVNAPSDAACDDSNPCTTDSCNP
                     ASGCVHTNNTAPCSDGSACTTNDACSGGACLGGPPPSCDDGNVCTDDSCIPSSGCAHA
                     NSTSSCDNGNGCCVGGVCTGGSPANHLVISQVQTQGDGVPLSDDEFVEIYNPTGSAIS
                     LSGLSLQFKSPMGATYLRVLLPALSIPSHGWFLVARSAYNGVPAADQIQAAFAMGGGG
                     NLFLVNGTTLLSGTCSTDVSIIDKVSYGGGTCPEGAVAPASGADNSIVRNPGGICGNG
                     WDTNDNSADFRVQVPSTPRNRFSTPQP"
     gene            complement(5834..>6182)
                     /locus_tag="VFD06_09990"
     CDS             complement(5834..>6182)
                     /locus_tag="VFD06_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03902.1"
                     /translation="ESLCLCPDPACPIGQRICNSERPTIPPADLVSEAFDSASNPAWV
                     LGLATISGNVLRLNGTSTPTSLAQVRFPTQPGQEYVVVVKWTSSPIVSGFQSCPDASL
                     LLSLETRPGSCGP"
BASE COUNT          894 a         2118 c         2252 g          918 t
ORIGIN      
        1 agtcgcctca cttcatcccg agctgctttg cagattgccg gtacaatgcg ccggcgcggc
       61 cgggctaccg ccccgcacgg ggaccacaac agcgtcgcgc gtcctgcgat gagggtcacc
      121 ccacttgacg gtcactgtcc ggctcctgtg catcgcggcg gcgtcgatgc tctggctcgg
      181 ccgggcgcac gcggccgctc ccggcgacgc ggccgctccc ggcgatcggc cggtgcttcg
      241 gcccggtgtc gagacgacgg tcaggctcga agggaaggac ggccgggcct tcaccctgaa
      301 gctcggcgcc ggagatcgcg tcgggttccg actcgtacgc cacgagggcc cgatcgcact
      361 ggtgctcgag gatccggccg cgcgcgagcg gcggcgctgg atccgcgacc cggccgacgg
      421 gtcggacacg atctgcccgc ggccgcggcg gccggcggcc gcgtggatcg ccgatgcggc
      481 cgggtcgtgg acgttgcgcg tcctgccacc cgaaggggtc ccaccgttcg aggtgacgtt
      541 ggttcgggat ccgccgcgca aggcgaccga catggatcgt ctggccttcg cggccgacct
      601 cgaggaggcg cgggtcgaag cgctcctcga agaagggaag ggtgccgaag cagccgaggc
      661 tgccaaggcc ctggtcgagg cccgcagccg cgaggaatca ggcgagccgc tgtcgctggc
      721 gagcgacctc ggctatctcg gagtgcactt ctatcagtcc gcgagctgcc gctccggagc
      781 gtgggacgtg gccgcggggc tcgtccgcca ggcgctggag ctccgtgccg ccgcattgcc
      841 cgcgggcgat ctctgcgtgg ccgaaagcct gagcctcgtt gcttcggtgc tctacgcgca
      901 gggccggttc gaggaggggg aaacgctcga gaagcgatcg ctcgcgatcc gcgaggtcgc
      961 acttcccgga gcgcgcgagt cctggcgcaa cagccttcgc gacgtcggac tgctcctgct
     1021 tcagcaggga aaattcgccg aggccgaagc gtatctcgtg cgggcccggg aggcggcggc
     1081 gaccacgggc ggagatgcgc cgcacgccct ggccgacgcg cggcacaacc tgggcgagct
     1141 gtaccggaac cagaatcgct acgacgcagc ggcggaggag ttgacgagcg ccgaagcgct
     1201 cgagcgggcg cagaccgatc ccgacctcgg actgctgtcc gacatcctcg ccgccctggc
     1261 cggggtgcat ctcgaccgtg ccgactacga cgaagccgag cggtacgcga agctgtacct
     1321 cgaccttctg gcacagcatg cggagatcgc ctccgccgac cacctggcca tcggaagggt
     1381 caatctcgga gagatctacc ggcgccaggg gcgactcggc gaggcggagc cgctgctcgt
     1441 cgaagccctg gccgctgcgc gcgagacctt ctgccccgaa aaccccaggc tgctctactt
     1501 ccggctggat gtggccctgc tctacagcga gcagggaaag cacgaggcgg ccgaggcgct
     1561 ctaccgcgaa tcgctgctca cgctcgagga ggcgctgggg ccgcggcacc cggacgtcgc
     1621 ggcgaatctc caggagctgg ccgggatgct cgccggtcgg gggcgcttca gcgaagcggt
     1681 gccgctctat gagcgggcgc ttgccattcg tgaggagacc ctcggtgcgg accatcctct
     1741 gacggccgcc agccgcacgc gcctggcgcg ggcacgcctg gacgggggag ccggcgactt
     1801 cgaggggctt ctcgcctccg cggtgcgagc ccggcgcgct ttggagacga gcgtgtccta
     1861 tcccgaggag gcggtcgacg ccttctcgtt cgaggccgac ctcctcgaca ggctcggcag
     1921 gaagcgcgag gcgcgccagg ccctcgccga agcgctcgcg cgcgtcgagc ggctccgtcc
     1981 gcgccgcggg ggcggggaga tggcccgcgt ggatttcctg cgccggcacc tcgacgacta
     2041 cgaccggatc gtggactggt ccctcgatgc cggcgacctc gacgctgcgt tcgcctatgc
     2101 cgagcggtcc agggcccggg ccctgctcga tcttttgacg gcgggaaccg gcgacttgag
     2161 aggctcgatg gccccggcgg tccggtcggt gctcgagaca cgggagaccg aggcgacgtc
     2221 cgagcttgcc gaggctcagg gccgcgccgc ctacgagcgt gcgcgcaccg atctcgatgc
     2281 ggcgcagcgc cgcgcgcgcc tcgccgatct cgagcgccag atcgatgcgg cggcgcgtcg
     2341 tctccaggtc gtggccgacg acttcaagaa caacagccct ctctggcgcg acgaagtcgg
     2401 cgcgcacggc gggccgatct cgcttccgga ggcccggcgc gatgtcgttc ctcctcacgg
     2461 ggtgatgctc gcgtatcgcg tcggccggct ggcgagcctc gtcttcgtga tccccccggc
     2521 ccccgcgaaa gtgatcgtgt cgcgcctcgt cgtttccgcc ggggatgcga cgaccctcgg
     2581 cattcccgcg gggccgctca ccgcgtcggc actgcagtcc gcgctcaccg gagatcccgc
     2641 agcgccggag attccgctcc tcgcccggat cgccgagccg cccggcgggc cgacggagga
     2701 cgcggccgcg acgatccgcg agctgcacgc actgcgccgg gtgctcgtcc ccgatgtggt
     2761 gcgggcccgc atgcggcgcg cgcaggaggt cgtcgtcatc cccgacggag cgctcgcgct
     2821 gctccccttc gaggtgctgg tcttcgaccc cgctcccgac tggtccggag ccaggctgtg
     2881 gctcgactcg ggtccgaccg tgcgctatgc ggcctcggcg acttcgctcg gaagtgtcat
     2941 cgcgcgtgcg gcgtcgcgcg ttcagggtcg gccgaccgtc ctgacggtgt ccgacccgat
     3001 cttcgatccg gcggagctcg atggcgcgag tcctccggcg gttccggtgg actcatcctc
     3061 cggaggacga cgtctgcagc gccttccagg aaccgcccgg gagagcgccg cggtcgaggc
     3121 cgcgttccgc gaggcgggat tcgtcgatgt ggtcacgcgc ctcgacgggg cgcgcgccac
     3181 ggaggcggcg gtacggatgg ccctgcccgg aaagcgcttc gtccacctgg ccacgcacgg
     3241 catcgtcaag ccgtcgcgca gcgacttgct cgccgggctc gcgctcacgg tgccgcgggg
     3301 cattccggcg agcgcggcgg aggacggcat gctccagctc ttcgaaatcg aagagcagaa
     3361 gctcgccgcg gagctcgtgg tcctgtcggc ctgcgacacg aacgtcgggc gccgcatcgc
     3421 cggcgaaggc gtgttctccc tggcgcgagg gtttttcgcc gccggcgccg gaagcgttgt
     3481 cgccagcctg tggcccgtgg acgacagctc gagcgcccgc ctcgtcggcg atctctttcg
     3541 cgggattgcc ggggcggcgg ccggtcgcga cgctcacccc gcggaggccc tgcgcgacgc
     3601 gaagcgaagg cttcgcgcca agcccgccac cgcggcgccg ttcttctggg ccgcgttcac
     3661 ctgcaccggg atccgctgag ccgcctcagg acctacggct gcggagtgct gaaccggttt
     3721 cggggcgtcg agggaacctg gacccggaaa tccgcggaat tgtcgttcgt gtcccacccg
     3781 ttgccgcaaa tccctccggg gttgcgaacg atgctgttgt ccgctcccga ggcgggggcg
     3841 acggcgccct ccgggcaggt ccccccgccg tagctgacct tgtcgatgat cgagacgtcg
     3901 gtcgagcagg tgccgctcag cagggtcgtg ccgttgacga gaaacaggtt tccccctccg
     3961 cccatggcga atgccgcctg gatttggtcg gcggcgggga ctccgttgta tgcggagcgg
     4021 gcgacgagaa accacccgtg ggacggaatg gacaaggcgg ggaggagaac cctgaggtac
     4081 gtcgctccca tcggcgattt gaattgaagg gagaggccgc tcaggctgat cgcgctgccg
     4141 gtcgggttgt agatctcgac gaactcgtca tcagagagcg gaactccgtc tccttgggtc
     4201 tggacctgcg agatcacgag gtggttcgcc ggagaaccgc cggtgcagac gccgcccacg
     4261 cagcacccgt tgccgttgtc gcacgaagac gtgctgttcg cgtgggcgca gcccgacgag
     4321 gggatgcacg aatcgtcggt gcagacgttg ccgtcgtcgc agctgggtgg cggccctccc
     4381 aggcaggctc cgccgctgca cgcgtcgttc gtcgtgcagg cgctgccgtc gctgcacggt
     4441 gcggtgttgt tcgtatggac gcagcccgac gccgggttgc aggaatcggt cgtgcaggga
     4501 ttcgagtcgt cgcacgccgc gtcgctcggc gcgttgacgc agccgaggga tgaatcgcac
     4561 gagtcgatcg tgcaggcgat ggtgtcgtcg cagctgcgcg catcctggat cctgggaccg
     4621 gccgtggcgt tccccgcggt cccttcgccg cacgagtccg ccgcgcgcac gaggtaccag
     4681 gcgagcgttc cgggcgcggg atccgccacg atcggcgccg aaagacccgg ggtgtccgcc
     4741 acgaggcagg agtccgcctt gacgttcagg aggttcggaa gatccgcccg gctcccggca
     4801 tagacccggt acgcgatccc ggcgggcgtg ccgccggggg gcaccggttg agcccagccc
     4861 agcgtcgtca cgcccccggc gatcgagaac gcgacgtcgc cgggctcacc ggggatcgcc
     4921 caggcctggc cgttccccgg atcgcagtcc gttccatcga atccgtcgtc gtcctggtca
     4981 caccagagtg gggcgcgcga ggcgaggaac agcgacaggt ccccggacgg gaagcacgtg
     5041 ctgacccatg ccgcgcgtgg atcgaccgcg cgcttcagga acagcacgta ggtctgacct
     5101 gcctgcacag cgaaacgcac cccggccgcc gcgatcgagg ctcccgaagg gagagccgcg
     5161 ccggttccga gcaccaggcg cgtatcgggc ggattgggcg cgatgctcga ctccatgaga
     5221 ctccagtggt tcgggttcgt cccgtcgtcg aagccgtcgt tgaaggcggc cacggcgggg
     5281 ccgaaaaccg catcggactc cggacacgcc gcgggaaggc caccgatgtc gcacaggcac
     5341 agagcctcgg cctgggggca ggtgtggcag ctccccggac tgccgcagta ggactgatcc
     5401 ggcggacagg tgactccggc gcacccggcg gtcccgatcg atgccccggc gcaggcggcg
     5461 agctcacaga acgagctctg cctgatcaca gcggcctgcc cgaggagcgc atccacgggt
     5521 ccctggcatc gcagcggggt tcccctgcag agggttcccg gaaggctggc ggcacactgg
     5581 gtgtccgtgg tgcagctcat cgtggacacg cacacggcgt tgacgcgact caggttcagg
     5641 atcgcctcgg agccctgggc cgtgaagaac acgtacgtcg gttcgtacgt gcggccattg
     5701 aggttcgacg acagacgctg cgagacatcc tgggcgacgg cgggaatcgt cgccgcgaac
     5761 agcaaggccg tggagagcgc tccaagtcgc gagcaggttc gcaacgccgt cgcggtccgc
     5821 atcgccctgg tcttcacggt ccgcacgagc ccggccgcgt ttcgagggag agcaggaggg
     5881 aggcgtccgg gcagctctgg aagccggaca cgatcgggga gctcgtccac ttcacgacga
     5941 cgacgtattc ctgcccgggt tgggtcggga agcggacctg cgcgagagag gtcggagtcg
     6001 aggtcccgtt gagccggagc acgtttccgg agatcgtggc caggcccagg acccaggcag
     6061 ggttcgaggc gctgtcaaac gcctcgctga cgagatcggc cggggggatg gtggggcgct
     6121 ccgaattaca gatgcgctgg ccgatgggac aggcggggtc ggggcacaaa cagagcgact
     6181 cg
//
LOCUS       DASRIQ010000408         4926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1982832, whole genome shotgun sequence.
ACCESSION   DASRIQ010000408 DASRIQ010000000
VERSION     DASRIQ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982832"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFD06_09995"
     CDS             complement(<1..391)
                     /locus_tag="VFD06_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZN03903.1"
                     /translation="MSRPGIRPAAVTAAFGAALALACAAVPEAVLAAAAAPAKPVLER
                     SVLKNGLTSYRLERAGLPLVQMQLLIPAGGTADPAGKEGLAALTARLLSRGSAGRTAA
                     AFAEEVEFLGGSLQADAGLDRTLIAGEF"
     gene            complement(388..1809)
                     /locus_tag="VFD06_10000"
     CDS             complement(388..1809)
                     /locus_tag="VFD06_10000"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZN03904.1"
                     /translation="MSSLAIWRRVVPLAMLLAAAPAAGVAEPDPSGRSAARANDAGPK
                     NAPAVTAGDLRFPITRERLPNGLEILIVEDHAVPVVTLYSFFRVGSRNERSGRTGISH
                     LFEHLMFNGAKRFGPGEFDRALESRGGAGNAFTSEDVTGYYEVFPPDALPLVVDLEVD
                     RLTGLTLTEDSLRSEREVVKEERRGRIDDDPMGALQELLGATAYVAHPYGWPVVGWPA
                     DLEAITVEDCREYFRIHYAPNNALLVLAGDVDPRSALAVLHRGYGSIPAQPPALPAVA
                     NEPEQAGERRAVLRRPAQLPAVALAFHVPGTATGGAEVFALDLIQTILTGGESSRLER
                     AVVRDKPLATSVVASNVYRVDPSLFIVQAEARPGVPLADLERGIAAEIERLAKEPVPD
                     RELAKAKNQTRMDLLRKLKTSAGKAEQLGMAAIYFGAPEKLFDLPQAFDAVTAENIRD
                     AAARWLVPDNRSVVHLVPAEPEP"
     gene            1977..2696
                     /locus_tag="VFD06_10005"
     CDS             1977..2696
                     /locus_tag="VFD06_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03905.1"
                     /translation="MTNRTLRRSVPMVLAAALTALCACHSDSNENDIKPIVASVSTPD
                     PVPSGPAVYLRVAASDNPSDDVVPIEVVLTPGATLTFDAFNVEILPTDPANPSILRDG
                     IVQMTFDTAMGSTPFGTCNSCVSTGGTCGVCVPCGSCPPTDPATNPVNTPFCFAATSS
                     SRSFLVGVANVVASGCLPETVTTDTVIAVLSVFARTVGSARLRFVVNPNVTGDCEILL
                     QTVVVPGVIFDDRGAVFTAAR"
     gene            2727..3122
                     /locus_tag="VFD06_10010"
     CDS             2727..3122
                     /locus_tag="VFD06_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03906.1"
                     /translation="MDEKVVAHYQDGRTLRGTTQDFRPESESFHLLPSEGGGIPTMVR
                     LTDLKALFYVKDYGYLRRQVERSRRFGSSAPGQKTIVEFRDGERLWGFTEEYTPGGHG
                     FFFTPADPQENNVRIFVINTSVKQITFED"
     gene            3127..3558
                     /locus_tag="VFD06_10015"
     CDS             3127..3558
                     /locus_tag="VFD06_10015"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HZN03907.1"
                     /translation="MSLLAARRLAVVLVLSVLALSSASAALNSAQYKFEGNRWLSLDL
                     AVGDVRTEVIRFEWPGAVLGIKTGYKARVKVVNGSARQVSAGIAVAVYDAAGKLIGAG
                     SSGTRIGTIDPGDSVDFDIEFNNVTEKLEQAAQFQIALQTR"
     gene            3673..4386
                     /locus_tag="VFD06_10020"
     CDS             3673..4386
                     /locus_tag="VFD06_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03908.1"
                     /translation="MSTTIRQSPARRSRSTALRLLATALLFAAAAPAAHAGVFYDASL
                     ALNISDDARVFLNMTNRHFGTPEPQAVSVLNRCPRPESDYPVVELFARLSGRRADVIV
                     AWRQKGLPWSEVMVRLQIPADRLFAGLDRDPGPPYGNAWGHWKKGQRGRGKKEPVTFD
                     DVTIADLARLQIASAALRVSPYTIIEERRRGVSVERFVVIRSQPSGVAAAKSNGSKPR
                     PEKGRGHGKDKLHPAQNPR"
     gene            4469..>4926
                     /locus_tag="VFD06_10025"
     CDS             4469..>4926
                     /locus_tag="VFD06_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZN03909.1"
                     /translation="MGMATDAIHAGVRPDPATGAVMTPIYQTSTYVYEGLGRGKGYDY
                     ARTINPTRSALEENLAAIEGGRAAYAFASGMSGIHAVTALLKSGDHVVVGNNVYGGTY
                     RLFSKVLEDFGLRFSYVDTSRLENVEGALTPATRMVYIETPTNPMMILTD"
BASE COUNT          746 a         1788 c         1657 g          735 t
ORIGIN      
        1 cgaattcccc cgcgatcagg gtccgatcga gccccgcgtc cgcctgcagg ctgccgccga
       61 ggaactccac ctcttcggcg aaggcggcgg cggtgcggcc cgccgacccg cgcgacagga
      121 gacgcgccgt cagcgccgcg agtccctcct tcccggcggg atcggcggtc cccccggccg
      181 ggatcaggag ctgcatctgc acgagcggga gtccggcccg ttcgaggcgg tacgacgtga
      241 gcccgttctt cagcaccgag cgttcgagga ccggcttggc cggcgccgcc gcggccgcga
      301 ggaccgcctc cgggacggcc gcgcaggcca gggccagcgc cgcgccgaag gccgccgtca
      361 cggcggccgg tcggatcccc ggccggctca cggctccggc tccgccggca cgagatggac
      421 cacgctgcgg ttgtcgggga ccagccagcg cgccgcggcg tcgcggatgt tctccgccgt
      481 gacggcgtcg aaggcctgcg gcagatcgaa caacttctcc ggcgctccga agtagatcgc
      541 ggccatgccg agctgttccg ccttgccggc gctcgtcttg agcttccgga gaaggtccat
      601 ccgggtctgg ttcttcgcct tggcgagctc ccggtcgggc accggctcct tcgccaggcg
      661 ctcgatctcg gccgcgatcc cccgctccag atccgcgagc ggaacccccg gacgcgcctc
      721 ggcctgcacg atgaacagcg agggatcgac gcggtagacg ttcgacgcca cgaccgacgt
      781 ggccagcggc ttgtcgcgca ccaccgcgcg ctccagtcgg gacgactccc cgccggtgag
      841 gattgtctgg atcagatcga gggcgaacac ctccgctccg cccgtggcgg tccccggcac
      901 gtggaacgcc agggcgacgg ccggcagctg cgccggacgg cgcagcacgg cgcgccgctc
      961 ccctgcctgc tccggctcgt tcgcgacggc cggcagtgcc ggcggctggg ccgggatcga
     1021 tccgtaaccc cggtggagga ccgcgagggc gcttcgagga tcgacgtccc cggccaggac
     1081 caggagggcg ttgttcggcg cgtagtggat gcggaagtac tcgcggcagt cctcgaccgt
     1141 gatcgcctcg aggtcggcgg gccagccgac gaccggccat ccgtaggggt gggccacgta
     1201 ggcggtcgcc ccgaggagct cctgcagcgc gcccatcggg tcgtcgtcga tccgcccgcg
     1261 gcgttcctcc ttcacgacct cgcgctcgct ccggagcgag tcttcggtga gggtgaggcc
     1321 ggtcaggcga tcgacctcca gatcgaccac gagcggcagc gcgtccggag ggaagacctc
     1381 gtagtagccg gtgacgtcct cgctcgtgaa ggcgttgccc gcgccgccgc gcgactccag
     1441 cgcccgatcg aactcccccg gaccgaaacg cttcgcgccg ttgaacatca ggtgctcgaa
     1501 caggtgggaa attccggtgc ggccggaccg ctcgttgcgc gatcccaccc ggaaaaaact
     1561 gtagagggtg accaccggga cggcgtggtc ctcgacgatg aggatctcga gtccgttcgg
     1621 caggcgctcg cgcgtgatgg ggaaccgcag atcgcccgcc gtcaccgcgg gggcgttttt
     1681 cggaccggcg tcgttcgcgc gcgcggccga acgtcccgac ggatccggtt cggcaacgcc
     1741 cgccgccggc gcggccgcga ggagcatcgc gagcggaacg acgcggcgcc agatcgccag
     1801 ggagctcacc tcgacatgtg tccgcccacg gcgggggcgg gatgatagca ccaaccgaat
     1861 gtccgccggc ggacaccgcg gggcggcggc cgcgccccgc cgccgtttcc gcgttgccgc
     1921 cccgcgatca cctcggtata cttcccgggc cctcccgaac gaggccaatc cttccgatga
     1981 caaaccggac ccttcgacgt tccgtcccga tggtgctcgc ggcagccctg acggccctgt
     2041 gcgcctgcca ctccgacagc aacgaaaacg acatcaagcc gatcgtggcc tcggtctcca
     2101 ctccggaccc cgtgccttcc gggcccgcgg tctacctgcg cgtggcggcg tccgacaacc
     2161 cgtcggacga cgtggtgccg atcgaggtgg tgctgactcc cggcgcgacc ctgaccttcg
     2221 atgcgttcaa tgtcgagatc ctgccgaccg acccggcgaa cccgagcatc ctgagggacg
     2281 gcatcgtgca gatgaccttc gacaccgcca tgggatcgac tccgttcggg acgtgcaact
     2341 cctgcgtctc caccggcggc acctgcgggg tctgcgtgcc ttgcggctcg tgcccgccga
     2401 ccgatccggc caccaacccg gtgaacacgc ccttctgctt cgccgcgacc agcagcagcc
     2461 gatccttcct ggtcggggtg gcgaacgtgg tggccagcgg ttgtcttccg gagaccgtga
     2521 ccaccgacac cgtgatcgcc gtcctcagcg tcttcgcccg gaccgtcgga tcggcccggt
     2581 tgcgtttcgt cgtcaacccg aacgtcaccg gcgattgtga gatactcctg cagaccgtcg
     2641 tcgtgccggg tgtgatcttc gacgatcggg gcgccgtgtt caccgccgcg cggtaaccag
     2701 accgcggcgt tccagggggg gaagcgatgg acgagaaagt cgtcgcgcat taccaggacg
     2761 gccgcacgct gcgcggcacc acgcaggatt tccgtccgga gtccgagtcg ttccatctcc
     2821 tcccgagcga agggggcggg atcccgacga tggtgcgcct cacggacctg aaggcgctgt
     2881 tctacgtcaa ggactacggt tacctccgcc gccaggtgga gcgctcgcgg cgcttcggat
     2941 cgagcgcccc cgggcagaag acgatcgtcg agttcaggga cggcgagcgg ctctgggggt
     3001 tcaccgagga gtacaccccc ggcggccacg gtttcttctt cacgccggcc gatccgcagg
     3061 agaacaacgt ccggatcttc gtgatcaata cctcggtcaa gcaaatcacc ttcgaggact
     3121 gacagaatga gccttctcgc ggcccgacgt ctcgccgtcg ttctggttct ctccgtcctg
     3181 gccctctctt ccgcctcggc ggcgctcaac tcggcgcagt acaagttcga gggaaaccgc
     3241 tggttgtcgc tcgacctcgc ggtgggggac gtgcgcaccg aagtcatccg cttcgagtgg
     3301 ccgggcgccg tgctcggcat caagaccggc tacaaggctc gggtgaaggt ggtcaacgga
     3361 tcggcccgcc aggtctccgc cgggatcgcg gtggcggtct acgatgccgc cggcaagctc
     3421 atcggcgccg gcagcagcgg cacccggatc ggcacgatcg atccggggga cagcgtcgat
     3481 ttcgacatcg agttcaataa cgtcaccgag aagctggagc aggcggcgca gttccagatc
     3541 gccctccaga cccgctagac cggttccccc gacgccgcgc cgccacccgg cgcggcgtcg
     3601 ccttgctccc cctggatccg cttcctatat tcgactcggg ccggatctcc gattcggccc
     3661 gggggggaag ccatgtccac gacaatccgc cagagtcctg cgcgccggtc ccgatcgacc
     3721 gccctgcgtc tgctcgcgac ggcgctgctc ttcgccgccg cagccccggc cgcgcacgcg
     3781 ggggtctttt acgacgccag cctggcgttg aacatctccg acgacgcgcg cgtcttcctg
     3841 aacatgacca accggcactt cgggacgccg gagccgcagg cggtgagcgt cctgaaccgc
     3901 tgcccgcggc ccgagagcga ttacccggtc gtggagctct tcgcccggct gtcgggccgc
     3961 cgcgccgacg tcatcgtggc ctggcgtcag aaagggcttc cctggtcgga ggtgatggtg
     4021 cggttgcaga tccccgccga ccggctcttc gccgggctcg accgggaccc cggcccgccc
     4081 tacggcaacg cctggggaca ctggaagaag gggcagcgcg gccgcggcaa gaaggagccg
     4141 gtgacgttcg acgacgtgac gatcgccgac ctcgcccggc tgcagatcgc ctccgcggcg
     4201 ctgcgcgtca gcccgtacac catcatcgaa gagcgccggc gcggcgtctc ggtcgagcgc
     4261 ttcgtcgtca tccgctccca gccgagcggc gtcgccgcgg cgaaatcgaa cggctcgaaa
     4321 ccccgccccg agaagggccg tgggcacggg aaggacaagc tgcatccggc gcagaacccg
     4381 cgctgacccg tactccctgg ccgaacccgg catatcatca ccgcgcaccc ggacctcatc
     4441 tcacagcgcc ggggcgaggt gacacttcat gggcatggcc accgacgcga tccacgcggg
     4501 cgtccgcccc gatccggcca ccggagcggt gatgacgccg atctaccaga cctccacgta
     4561 cgtctacgaa gggctcgggc gcggcaaggg gtacgactac gcgcgcacca tcaacccgac
     4621 ccgctcggcg ctggaggaga acctggcggc gatcgagggg gggagggcgg cgtacgcctt
     4681 cgcctccggc atgtccggca tccacgccgt cacggcgctg ctcaagtcgg gggatcacgt
     4741 cgtcgtgggg aacaacgtct acggcgggac ctaccggctg ttctcgaagg tgctcgagga
     4801 cttcggtctg cgcttcagct acgtcgacac gtcccgcctc gagaacgtcg aaggggcgct
     4861 gaccccggcg acgcgcatgg tctacatcga gaccccgacc aacccgatga tgatcctcac
     4921 cgacat
//
LOCUS       DASRIQ010000409         5190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2489928, whole genome shotgun sequence.
ACCESSION   DASRIQ010000409 DASRIQ010000000
VERSION     DASRIQ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489928"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFD06_10030"
     CDS             <1..590
                     /locus_tag="VFD06_10030"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HZN03910.1"
                     /translation="VVRDGQYLPGGGRTFRAWIHDGLDGERPTLHDFETHLTTLFPEV
                     RLKRYIELRGADSGDPRAVLALGALWKGLLYDPGARAAAWDLVRDASFVERNALLDAV
                     CRDGPAAPLPDHGRAGAPRRVGDLHSEILGLARRGLAAQGAGAEARWLAPLERIVADG
                     PGCPALELLHEWDATAGDARTLVDTLSRNTLADAA"
     gene            622..1533
                     /gene="ftcD"
                     /locus_tag="VFD06_10035"
     CDS             622..1533
                     /gene="ftcD"
                     /locus_tag="VFD06_10035"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HZN03911.1"
                     /translation="MPLIESVPNFSEGRRPDVVEAIVEAVRSSGAIRVLDRSSDPDHN
                     RTVLTLAGEPEPLLAGLEALVAACVERIDLTRHSGRHPRMGAVDVVPLVPIRDVTLSE
                     CAGYARALGERVARRHDLPVFLYEEAASAPHRRDLASIRKGEFEGFADKIRDPLWKPD
                     FGPDRVHPTAGCVAIGAREFLIAFNINLTTSDVEVARRIAQAIRHSGGGLRYVKAMGL
                     LLEDRRQAQVSINMTDFRRTPLHRVFEMVRSEAERYGVAIAGSEIVGLVPQDALLQTA
                     EHFLRLEKFDSGLLFERRLETTAAGDR"
     gene            complement(1543..3555)
                     /locus_tag="VFD06_10040"
     CDS             complement(1543..3555)
                     /locus_tag="VFD06_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN03912.1"
                     /translation="MSQVHADGPVQAVASGPVAGSKKRAGTGALRRRTRALRPTVVVG
                     NGLKGAANLAWHAFQAVNRRVPFGSVQPKWAPAPLLKSHERSFPELGFPRETDSLCPE
                     CVKALRARILNGEADWKTLLQGKPGEVRARIVERDGQVWMEKTCPQHGPCEDIMAMDS
                     EFLRRMERLYPGRDFRMKPDPYHNHGSSTVKYGRGAVLTVDLTNRCNMMCNPCFMDAN
                     QVGYVHELSWDDVRQILDNSIQVTPRRQMSVQFSGGEPTISPYFLDAVAYARKVGYQS
                     VQCATNGIRFAQDIDYCHRAKAAGLRLAYLQFDGVTNEANNHRGVGNLFDVKKRALEN
                     LKIAGIDVTLVVTIVNTVNNDQVGPIIRFAIDNVDKINAVSFQPVSFTGRDEDIDDET
                     RRKQRYTLSHLAHDVKSQLGVTEPLRDWYPLSASGPFSDLRDQLDGIEAEFGSLKCGC
                     HPNCGIGTMLLVNEQTKQAVPFPQFLDTDRLLEDLKIITDTCRPKTLTVAQFAMAVLR
                     NLRPSELPEGLTLWELIKIIDGHNGARLGLAEKARYNWRIMMVAGMWFQDLFNYDFRR
                     TEMCIIPYGTQVGEVSFCAYNTGVGWRQIVEKMFSTHSTAQWFKEKGRHPIYAAGKNV
                     PLEAGARPIPADAIGRPVREEVPALAGAAASAAARPVSGAAAHPGH"
     gene            complement(3890..>5190)
                     /locus_tag="VFD06_10045"
     CDS             complement(3890..>5190)
                     /locus_tag="VFD06_10045"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN03913.1"
                     /translation="EGYQVDAAESGERGLEMARRHPYHAVVLDVWLPGRDGLAVLEDL
                     AALPSPPRVVMISGHADVPVAHKAGKLGAYDFIEKPLSLDKVILTLRNAIADRRKEAR
                     LNALKDRVPEEETLLGDSPAIRDLQAQIELAAPTQGRVLITGENGTGKELVARLIHAR
                     SLRRDELFVEMNCAAIPEELIESELFGHMKGAFTGATEGKAGRFLLADGGTLFLDEIG
                     DMSLRTQAKVLRVLQEQAFEPVGGSTVRVDVRVLAATNKDLPKEIASGRFREDLYFRL
                     NVVPLRVPALRERTGDIPILARHFVASLSRQYGRTMVLEDGAIPPLVDYPWPGNVREL
                     RNLMERLMILAPGPAIAAVAVLAALQPERGHRPSEPERDFESLKEGREDFERRFIARK
                     LRESSGNVVRAAQLLGLERSHLYRKMRAYGLRTGVGEEEV"
BASE COUNT          747 a         1818 c         1798 g          827 t
ORIGIN      
        1 tcgtcgtacg cgacggacaa tacctcccgg ggggcgggcg cacgttccgc gcctggatcc
       61 acgatgggct cgacggggag cggccgacgc tccacgactt cgagacgcac ctcacgacgc
      121 tcttcccgga ggtgcggctg aagcggtaca tcgagctgcg cggggccgac tccggcgatc
      181 cgcgcgccgt gctcgcgctc ggggcgctgt ggaaggggct cctgtacgat cccggagccc
      241 gcgccgccgc gtgggacctg gtgcgcgatg cgtcgttcgt cgagcgcaac gcgctgctcg
      301 acgccgtctg ccgggacggc cccgcggcac cgctcccgga tcacggccgg gccggagcgc
      361 cccgtcgagt cggggacctg cactccgaga tcctggggct ggcgcggcgc ggcctcgcgg
      421 cgcagggagc cggcgcggag gcccgctggc tcgccccgct cgagcggatc gtcgccgacg
      481 gtccgggatg tccggcgctg gagctgctgc acgagtggga cgccaccgcc ggggatgcgc
      541 ggaccctcgt ggacacgctg tcccgcaaca ccctggcgga cgccgcctga tccgcggcgg
      601 cgccattcga acggagccgc gatgcccctc atcgagagtg tgccgaactt cagcgaagga
      661 cggcggccgg acgtcgtcga ggcgatcgtc gaggcggtgc ggagcagcgg agcgatccgc
      721 gtgctcgacc gctcctccga tccggaccac aaccgcaccg tcttgacgct ggccggcgag
      781 ccggagccgc tcctcgcggg cctcgaggcg ctggtggcgg cctgcgtcga gcggatcgac
      841 ctgacgcggc acagcggccg tcatccccgc atgggggcgg tggacgtcgt ccccttggtg
      901 cccatccgcg acgtgaccct gagcgagtgc gccggttatg cccgcgcgct gggcgagagg
      961 gtggcgcgcc gccacgacct gccggtcttt ctgtacgagg aggcggcgag cgccccgcac
     1021 cggcgcgatc tggcctccat ccgcaagggg gagttcgagg ggttcgccga caagatccgt
     1081 gacccgctgt ggaagcccga cttcgggccg gatcgggtcc acccgaccgc cggttgcgtc
     1141 gccatcggcg cccgcgagtt cctgatcgct ttcaatatca acctgacgac gtccgacgtg
     1201 gaggtcgccc ggcgcatcgc ccaggcgatt cgccactcgg gcggcggcct gcggtacgtc
     1261 aaggcgatgg ggctgctgct ggaggaccgg cggcaggccc aggtgtcgat caacatgacc
     1321 gacttccggc gcacgccgct gcaccgcgtc ttcgagatgg tgcggtcgga ggcggagcgc
     1381 tacggcgtgg cgatcgccgg gagcgagatc gtgggactgg tgccgcagga cgcgctgctc
     1441 cagacggcgg agcacttcct ccgcctggag aaattcgatt cggggctgct tttcgagcgc
     1501 cgcctcgaga ccacggccgc cggggatcgc tgagcccgat cctcagtgac cggggtgcgc
     1561 cgcggccccg gagaccggac gggccgccgc cgacgccgcc gcgccggcca gggccggaac
     1621 ctcctcgcgg accgggcgcc cgatggcgtc cgccggaatg gggcgcgcgc cggcctcgag
     1681 gggcacgttc ttccctgccg cgtagatcgg gtgccgtccc ttctccttga accattgcgc
     1741 cgtgctgtgc gtcgagaaca tcttctcgac gatctgccgc cagccgacgc cggtgttgta
     1801 ggcgcagaac gagacctccc ccacctgcgt cccgtagggg atgatgcaca tctcggtgcg
     1861 gcggaagtcg tagttgaaca ggtcctggaa ccacatcccg gcgaccatca tgatgcgcca
     1921 gttgtagcgg gccttctccg ccaagccgag ccgcgccccg ttgtgaccgt cgatgatctt
     1981 gatcagctcc cagagggtca gcccctcggg cagctcggag ggacgcaggt tgcgcagcac
     2041 cgccatggcg aactgcgcca ccgtcagcgt cttcggccgg caggtgtcgg tgatgatctt
     2101 gaggtcttcc agcagccggt cggtgtcgag gaattgcggg aacgggaccg cctgcttcgt
     2161 ctgctcgttc accagcagca tcgtcccgat gccgcagttg gggtggcagc cgcacttgag
     2221 cgatccgaac tccgcctcga tgccgtccag ctgatcccgc aggtccgaga acggccccga
     2281 cgccgacagc gggtaccagt cgcgcagcgg ctcggtcacc cccagctggc tcttcacgtc
     2341 gtgcgccaga tgcgacaggg tgtagcgctg cttgcgccgc gtctcgtcgt cgatgtcctc
     2401 gtcgcggccg gtgaacgaca ccggctggaa cgagacggcg ttgatcttgt cgacgttgtc
     2461 gatggcgaag cggatgatcg ggcccacctg gtcgttgttg accgtgttga cgatggtgac
     2521 gacgagcgtc acatcgatcc cggcgatctt gaggttctcc agggcccgct tcttgacgtc
     2581 gaacaggttg ccgacgccgc ggtggttgtt cgcctcgttg gtgactccgt cgaactgcag
     2641 gtacgccagc ctcagccccg ccgccttggc gcggtgacag tagtcgatgt cctgcgcgaa
     2701 gcggatgccg ttggtggcgc actgcacgct ctggtagccc accttgcggg cgtacgcgac
     2761 ggcgtcgagg aaatacggcg agatggtcgg ctcgccgccg ctgaactgca ccgacatctg
     2821 ccggcgcggc gtgacctgaa tcgagttgtc gagaatctgg cgcacgtcgt cccacgacag
     2881 ctcgtggacg tagcccacct ggttcgcgtc catgaagcac gggttgcaca tcatgttgca
     2941 gcggttggtc agatcgacgg tgagcaccgc gccccgaccg tacttcacgg tggacgaccc
     3001 gtggttgtga taggggtccg gcttcatccg gaaatcgcgc cccgggtaga ggcgctccat
     3061 gcgccgcagg aactccgagt ccatcgccat gatgtcctcg cacggaccgt gctgcgggca
     3121 ggtcttctcc atccacacct ggccgtcccg ctcgacgatg cgcgcccgaa cctcgcccgg
     3181 cttcccctgc aggagcgtct tccagtccgc ctcgccgttg aggatgcggg cccggagcgc
     3241 cttgacgcac tcagggcaga gggagtcggt ctcgcgcggg aatcccagct cggggaacga
     3301 gcgctcgtgg ctcttgagca ggggcgcggg ggcccacttc ggttgcacgc tgccgaacgg
     3361 gacacgacga ttgaccgcct ggaaggcgtg ccaggcaagg ttcgccgccc ccttcagccc
     3421 gttccccacg acgaccgtgg gccgcagggc gcgggtccgt ctgcggagcg cgccagtccc
     3481 cgcccgcttc ttcgaaccgg ccactggacc ggaagccacc gcctgaaccg gaccgtccgc
     3541 gtgaacctga ctcattcgcg atcctccatg ctgattcgag agccggatgc gcagtcgccg
     3601 ccgcggaacc ggctcgagga gtctgaaacc cgttgaagca agaacccgtg cgtcaggacg
     3661 gggcttccga cacatccgcc gccgtcattg gtccctggac ccgccatcga atgcacggtc
     3721 ccatcgaggg tcgcggtgcg ccttggcgcg cccgccactc cccatcggcc cggttgcccg
     3781 ggcctccgcg acaaaaggcc aaccgcttta ttatcagagg gaagtacgcg agtcaaggga
     3841 gtggcgtggg tagggggagc tccgactcgc tccccgatgg gggggacgct cagacctcct
     3901 cctcccccac cccggtccgg aggccgtagg cgcgcatctt ccggtagagg tggctccgct
     3961 caaggcccag gagctgggcg gcccgtacga cgttgccgga cgattctcgc agcttgcggg
     4021 cgatgaaccg gcgctcgaag tcctcccggc cctccttgag ggattcgaaa tcccgttccg
     4081 gctcggacgg ccggtgtccc cgctcgggct ggagcgccgc cagcaccgcc accgccgcga
     4141 tcgccggtcc gggcgccagg atcatcagcc gctccatcag gttgcgcagc tcgcgtacgt
     4201 tcccaggcca ggggtagtcg accagggggg ggatcgcccc gtcctcgagc accatggtcc
     4261 ggccgtactg gcgcgacaag gacgcgacga aatgccgcgc caggatcggg atgtccccgg
     4321 tcctctccct gagcgccggg acccggagcg gcacgacgtt cagacggaag taaagatcct
     4381 cgcggaaccg gccggaggcg atctctttcg gcaggtcctt gttcgtcgcc gccaggacgc
     4441 gcacgtccac ccgcaccgtg gatcctccga ccggctcgaa cgcctgctcc tgcaggaccc
     4501 gaaggacctt tgcctgggtc ctgagggaca tgtcgccgat ctcgtccagg aacagggtgc
     4561 cgccgtcggc gaggaggaac cgcccggcct tcccctcggt cgctccggtg aaggcccctt
     4621 tcatgtggcc gaagagctcg ctctcgatca gctcttccgg gatcgcggcg cagttcatct
     4681 ccacgaacag ctcgtcgcgc ctcagggacc gggcatggat gagccgggcc accagctcct
     4741 tgccggtgcc gttctcgccg gtgatcagca cgcgcccctg ggtcggcgcg gccagctcga
     4801 tctgcgcctg cagatcgcgg atcgccgggc tgtcgccgag gagggtctcc tcctccggca
     4861 cccggtcctt cagcgcgttc agccgggcct ccttgcggcg atcggcgatg gcgttgcgca
     4921 gggtgaggat caccttgtcg agcgacaggg gcttctcgat gaagtcgtag gccccgagct
     4981 tgccggcctt gtgggcgacc ggcacgtcgg cgtgccccga gatcatcacg acgcgcggcg
     5041 gcgacggcag ggcggcgagg tcctcgagga cggcgagccc atcccggccg gggagccaga
     5101 cgtcgaggac cacggcgtgg tagggatgcc gccgcgccat ctccagccca cgctctcctg
     5161 attcggcggc gtccacctgg tagccctcgt
//
LOCUS       DASRIQ010000410         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_170341, whole genome shotgun sequence.
ACCESSION   DASRIQ010000410 DASRIQ010000000
VERSION     DASRIQ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170341"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFD06_10050"
     CDS             complement(<1..486)
                     /locus_tag="VFD06_10050"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZN03914.1"
                     /translation="MRGPAAGLRRLKDLAGRGYRAIAGVDEVGRGCLAGPVVAAAVIL
                     DDARPIRGLRDSKLLTAPDRERLDRRLRDRARAVGVGVADEREIDALNILRATLLAMR
                     RALEALGVRPDYVVVDALTLPGLPIPQEGVVRGDRTCAPIAAASIVAKVHRDGLMRRL
                     HE"
     gene            complement(497..838)
                     /gene="rplS"
                     /locus_tag="VFD06_10055"
     CDS             complement(497..838)
                     /gene="rplS"
                     /locus_tag="VFD06_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZN03915.1"
                     /translation="MDVIKMIEDREIKTQPPAFRVGDSVKVHVKVREGEKDRIQVFNG
                     LVIAMKGGGTGSTFTVRKVSDGIGVERIFPLYSPVIGKIEVTKHGRVRRAKLYYLRTR
                     KGKAARIEERD"
     gene            complement(876..1589)
                     /gene="trmD"
                     /locus_tag="VFD06_10060"
     CDS             complement(876..1589)
                     /gene="trmD"
                     /locus_tag="VFD06_10060"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZN03916.1"
                     /translation="MTFDVVTAFPGMLEGPLGFSILKRAREAGIVTIRVHDLRAWAED
                     RHHKVDDAPYGGGGGMVLMPGPLFGAVEDIRLRHPAKRDRTILFCPQGIRYDQEAARR
                     LSGYDRLILICGRYEGVDERVREHLADESISIGDYVLTGGEIPALAVIDSVTRLLPGA
                     LGNEDAPRRDSFWDDRLDHPHYTRPAEFRGHKVPEVLLSGDHGKIAAWRREQAQKRTA
                     ERRPDLLERRREDDEATKH"
     gene            complement(1586..2128)
                     /gene="rimM"
                     /locus_tag="VFD06_10065"
     CDS             complement(1586..2128)
                     /gene="rimM"
                     /locus_tag="VFD06_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZN03917.1"
                     /translation="MAAEPRPALIAVATGLRLRGAGGRLTATLVPGGIDQLQGRRSVF
                     VGHSALESGREMEIESSTTYGRRLAIKFRGVDDAAAAEALAGLDLLLPAASLQDLPDG
                     SYYIHELVGMQVRTRAGRIVGTVRRVLEAGGAAPLLAVVPDGGADEVLIPAAQSICVA
                     IDRAARTVIVDPPEGLLEIE"
BASE COUNT          293 a          819 c          748 g          322 t
ORIGIN      
        1 ctcgtgcagg cggcgcatga gaccgtcccg gtggaccttg gcgacgatgg aggcggcggc
       61 gatgggggcg caggtccgat ccccccggac gaccccctcc tgggggatcg gcaggccggg
      121 cagggtgagg gcgtccacga ctacgtaatc ggggcggacg ccgagcgcct cgagggcccg
      181 gcgcatggcc aggagggtgg cgcggaggat gttgagggcg tcgatctccc gctcgtcggc
      241 cacgccgacg ccgacggcgc gggcccgatc ccggaggcga cggtcgagcc gctcccggtc
      301 gggggcggtc agcagcttgg aatcgcgcag gccgcggatc ggccgcgcgt cgtcgaggat
      361 gaccgccgcg gccaccaccg ggccggcgag acacccccgc cccacctcgt ccacgccggc
      421 gatggcgcga tagccgcgcc ccgccaagtc cttgagccgc cgcagtcccg cagccggacc
      481 gcgcatggga aggagctcag tcccgctcct cgatgcgcgc cgccttcccc ttgcgggtgc
      541 gcaggtagta cagcttggcg cgccggacgc ggccatgctt ggtcacctcg atcttgccga
      601 tgaccggcga gtagagcggg aagatccgct ccacgccgat gccgtcggag accttgcgca
      661 ccgtgaaggt actcccggtg cctcccccct tcatggcgat gacgaggccg ttgaagacct
      721 ggatgcggtc cttctccccc tcgcgcacct tcacgtgcac cttcaccgag tccccgaccc
      781 ggaaggcggg cggctgggtc ttgatttccc tgtcctcgat catcttgatg acgtccatgg
      841 ttccctcgct ccttgcgttg tccgtgccgt gccgttcagt gcttcgtcgc ttcgtcgtcc
      901 tcgcgccggc gctcgagcag gtccggccgc ctctcggccg tacgcttctg cgcctgttcc
      961 cgcctccagg cggcgatctt cccgtgatcc cccgacagca ggacctccgg caccttgtgg
     1021 ccgcggaact ccgccggccg cgtgtagtgc gggtgatcga gccggtcgtc ccagaacgaa
     1081 tcgcgccggg gcgcatcctc gttccccagg gcgccgggga gcaggcgcgt caccgagtcg
     1141 atgaccgcca gcgccgggat ttccccgccg gtcagcacgt agtcgccgat ggagatcgac
     1201 tcgtcggcga ggtgctcgcg cacccgctcg tccaccccct cgtagcggcc gcagatcagg
     1261 atcagccgat cgtacccgga gagacggcgg gccgcctcct ggtcgtagcg gatcccttga
     1321 ggacagaaga ggatggtccg gtcgcgcttc gccgggtgcc gcaggcggat gtcctccacc
     1381 gcgccgaaca gcgggccggg catcaggacc attcccccgc ccccgccgta gggggcgtcg
     1441 tccaccttgt gatgcctgtc ctcggcccag gcgcgcaggt cgtgcacacg aatcgtcacg
     1501 atcccggcct cgcgggcacg cttcaggatg ctgaagccga gcggcccctc gagcatcccc
     1561 gggaaggcgg tgaccacgtc gaaggtcatt cgatctccag gagcccctcg gggggatcga
     1621 cgatgaccgt gcgggcggcg cgatcgatgg cgacgcagat cgactgcgcc gccgggatga
     1681 ggacctcgtc cgccccgccg tccgggacca ccgcgagcag gggggccgct ccgccggctt
     1741 cgaggacccg ccgcacagtt ccgacgatcc gtcccgcgcg cgtgcgcacc tgcatgccga
     1801 cgagctcgtg gatgtagtac gagccgtccg gcaggtcctg gagactggcc gcgggcagca
     1861 ggaggtccag gccggcgagc gcctcggcgg cggccgcgtc gtcgacgccg cggaacttga
     1921 tcgccagccg ccggccgtag gtcgtgctcg actcgatctc catctcccgt ccgctctcca
     1981 aagccgaatg ccccacgaac accgagcgcc ggccctggag ctggtcgatg ccgcccggga
     2041 ccagggtcgc ggtcaggcgc ccccccgccc cgcgcaggcg gagccccgtc gccaccgcga
     2101 tcagcgccgg gcgcggctcc gccgccaccg cctcactcct cgtcgacgat ctcaagctcg
     2161 tacaccgccc cgtgtttctc gg
//
LOCUS       DASRIQ010000411         6092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3276772, whole genome shotgun sequence.
ACCESSION   DASRIQ010000411 DASRIQ010000000
VERSION     DASRIQ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3276772"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFD06_10070"
     CDS             <1..837
                     /locus_tag="VFD06_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03918.1"
                     /translation="VGRGAPHAHGEGFLLLAKSSSLDLTDPFKDVRLTLDLEKEEIPD
                     LSVYNVYFPKASRARIVSGSGRMSWHFEADAHEHSLQGEILLDMRDLAMKFEETTLVG
                     DATIRTRLKHGDPRSRTFDISGTSVTLRHRDPLWRGVLRFPRAVLAFTQPMRIDARTS
                     LELQDTLPLVKVFDAFKNVPPRIERLLTIEDVTGGTGFIVRPDVVEIHDLAIRGKGLK
                     AQAELTLQARGKEGILYIRWHGFSLGILLQPGKEKDLKLVRPLAWFEREKAARRKAAA
                     SR"
     gene            950..1732
                     /locus_tag="VFD06_10075"
     CDS             950..1732
                     /locus_tag="VFD06_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HZN03919.1"
                     /translation="MLLQGKTALIVGVANKRSIAWGIAEAFHRQGATLAFNYQGERLK
                     ENVEGLAAAMPGSLVVPCDVTKDAEIDAMFAAVKEHLGRLDILVHCVAFAKKEDLEGS
                     FATTSRDGFAMALDISAYSLTALAQRAAPLMSGEGPDAPGGAILALTYLGADRVVPKY
                     NVMGVAKAALESSIRYLAWDLGKRNIRVNGISAGPINTLAARGIAGFTSILQHVREKS
                     PLQRNTEPAEVGDAAVFLCSDLGRGVTGEVLFVDNGYHIMGT"
     gene            1742..2782
                     /locus_tag="VFD06_10080"
     CDS             1742..2782
                     /locus_tag="VFD06_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN03920.1"
                     /translation="MPLPPRRTAPSEPPVAMALVAEGLSKTYRSGVGRRPVQALDRLD
                     LKVPRGVVFGLLGPNGAGKTTLVKVALGIARATAGRAELLGRPAGDVPIRARIGYLPE
                     NHRYPAHLSGEQVLHFFGRLSGLAEPDRSRRVEHLLRRVRMDEWRTTPVRRYSKGMMQ
                     RLGMAQAILNEPDLLILDEPTDGVDPVGRREIRDLLLEQKERGATIFLNSHLLSEVER
                     LCDRVAILKGGRLVREGHVEDLTRGGAVNVWEIDVQGGDDARLAAVMGPAALRPAGPP
                     PLPGAAAVAREGGAAVRDGVRTLEVSGETEDLNRAIDALRAAGLTIAAVRARRESLED
                     VFVKIVRDGEAA"
     gene            2811..3698
                     /locus_tag="VFD06_10085"
     CDS             2811..3698
                     /locus_tag="VFD06_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN03921.1"
                     /translation="MNPGAKVAAIVLDTGRELAYRKTLLFYFGIVTLTHLFLLLALQT
                     DVANGVITSIRLFGLEGSVHGFGGRAAEGMGLEANQLVRGIQIAVTWTLYPIGVMLAV
                     FATASLVPHMLEKGTIDLLLSKPVSRPVLFVSRYLGALLVAGANLFYFVGGVGVILAI
                     KTGVWNGGFFLSGLLMTVYFGALLAFLVLIGVLLRSTTIGIMSAAAVYFVSLIVAFPH
                     ANADWPALLTSKPARWGTQAVVEVLYHALPRTLELGNMVTALVMQRPVASWMPVAWTI
                     VSGAGALLLAIAWFRRCDF"
     gene            3710..4780
                     /locus_tag="VFD06_10090"
     CDS             3710..4780
                     /locus_tag="VFD06_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03922.1"
                     /translation="MRATRHGGSDGTSRGAAGIAVVVALAVVAAVAGGGCSRTPIDAA
                     SAVKQSDLRFLPGKTVGLAVVEVARFEDKAAIGRWLDDFAKQAGAGGLDRVRATLGPD
                     ALDRLDRIALALVPAAGSTQYVVLAEGRFEEQTVKNLTGGGGIVTLVEVGTAPDLSIM
                     PLPKNHLALGPRAVLEQVRVLAAGGGGDQGLAGAPVMKVLSRVNPAKQIWGGIDYSVL
                     AGLLEEGSRDGGGSPMAGFARSLRALAFQGTIDDGVQFDLIGLADGEPGAKTLADAAR
                     GLVAIARVGASQGANRAAEGSSGGAADPAVESAQAWRRFLDGVRIDQERDEIRVHGTL
                     DGPMARSFAGAATRAVPGVGTN"
     gene            complement(4815..5345)
                     /locus_tag="VFD06_10095"
     CDS             complement(4815..5345)
                     /locus_tag="VFD06_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HZN03923.1"
                     /translation="MARRPAPRPAPPGPRHRVDWHTYFMNIATQVATRSTCDRKHVGA
                     VIVRDKTILSTGYNGSIRGMPHCDEVGHDLENDHCVGTIHAEANAILQAAKNGVMIDG
                     AELYTTASPCWSCFKLIANAGIRTIYYGEFYRDRKSLALARRLKIALIDLGEAARKAA
                     RSTPEEAGCDWKAPAD"
     gene            complement(5356..6003)
                     /locus_tag="VFD06_10100"
     CDS             complement(5356..6003)
                     /locus_tag="VFD06_10100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN03924.1"
                     /translation="MSSTAKGATASRLLTLTACAALLAGVALAQTPPAEPKKESAPVT
                     SAPAGEAKKKDVAVIKTSMGTIVFEFLPDVAPKMVANFKDLAKSSFYNGTTFHRVING
                     FMIQGGDPLSKDDDPNNDGTGSGPRKMPAEFSTKYSHVRGTVSTARGGNDINSGSCQF
                     FIVQKDSKFLDNQYTLFGRVLEGMDVVDKISLVPKGKADRPLKNVTMESVTIETR"
BASE COUNT          842 a         2045 c         2245 g          960 t
ORIGIN      
        1 gtcgggcgcg gcgccccgca cgcccacggc gagggattcc tcctgctcgc gaagagttcc
       61 agcctcgacc tcaccgaccc gttcaaggac gtgcgcctga cgctcgatct cgagaaggag
      121 gagatcccgg atctctccgt ctacaacgtc tatttcccga aggcgagccg ggcgcgcatc
      181 gtctcgggct ccgggaggat gtcgtggcac ttcgaagcgg acgcgcacga gcactcgctg
      241 cagggcgaga tactcttgga catgcgggac ctggcgatga agttcgagga gacgaccctc
      301 gtcggcgacg cgaccatccg cacgcgtctt aagcacggcg atccgcgcag ccgcaccttc
      361 gacatctccg gcacctccgt gaccctgcgg caccgggacc cgctgtggcg cggcgtgctc
      421 cgcttcccgc gcgccgtcct ggccttcacg cagccgatgc gcatcgacgc gcgcacgtcg
      481 ctcgaactcc aggacaccct gccgctcgtg aaggtcttcg acgccttcaa gaacgtcccg
      541 ccgcggatcg agcgactctt gacgatcgag gacgtgaccg gcgggaccgg cttcatcgtc
      601 cggcccgacg tggtcgagat ccacgacctc gcgatccggg ggaaggggct caaggcgcag
      661 gccgagctga cgctccaggc ccgcggcaag gaggggatcc tctacatccg ctggcacggc
      721 ttctcgctgg ggattctcct ccagcccggg aaggagaaag atctcaagct cgtccggccg
      781 ctggcgtggt tcgagcggga gaaggcggcc cgacggaaag ccgccgcctc ccgctgaggc
      841 gggatcccgg gaaccgcccg gcgggcgcgg gcgttccacc ccgcgattcg gatacgatgg
      901 cgccgcggat ccttcctcgt cgcgaggatc ggatccgggg aggtgaacga tgctcctgca
      961 gggaaagacg gcgctcatcg tcggcgtcgc caacaagcgc agcatcgcct gggggatcgc
     1021 cgaggcattc caccgccagg gggcgacact ggccttcaac taccaggggg agcggctgaa
     1081 ggagaacgtc gaggggctcg cggccgccat gcccgggtcg ctcgtggtgc cgtgcgacgt
     1141 cacgaaggac gccgagatcg acgcgatgtt cgcggcggtg aaggagcacc tcggccggct
     1201 cgacatcctg gtgcactgcg tcgccttcgc caagaaggag gacctcgagg gctcgttcgc
     1261 caccacgtcg cgcgacgggt tcgcgatggc gctcgacatc agcgcctact cgctgacggc
     1321 gctggcgcaa cgggcggcgc cgctcatgag cggggagggg ccggacgcgc ccggcggcgc
     1381 gatcctggcg ctcacctacc tcggggccga ccgggtggtg ccgaagtaca acgtcatggg
     1441 ggtggcgaag gcggcgctcg agtcgagcat ccgctacctc gcctgggacc tcggcaagcg
     1501 caacatccgg gtgaacggga tctccgccgg gccgatcaac accctggcgg cccgcggcat
     1561 tgccggcttc acctcgatcc tccagcacgt gcgggagaag tcgccgctgc agcgcaacac
     1621 cgagccggcg gaggtggggg acgcggcggt gttcctctgc tccgatctcg gccgcggcgt
     1681 caccggcgag gtcctcttcg tggacaacgg ctaccacatc atggggacct aggagaaccg
     1741 cgtgccgctg ccgccacggc gcaccgcgcc gtccgagccg cccgtcgcga tggcgctggt
     1801 cgccgagggg ctgtcgaaaa cctatcgatc cggtgtgggg cgccggccgg tgcaggcgct
     1861 cgatcgcctc gatctgaagg tgccgcgtgg cgtcgtgttc ggactcctcg gtccgaacgg
     1921 tgcggggaag acgacgctcg tgaaggtggc gctcggcatc gcgcgggcga ccgccggacg
     1981 ggccgaactg ctcggccgtc cggcggggga cgtgccgatc cgggcgcgga tcggctacct
     2041 gccggagaac caccgctacc cggcgcacct ctccggcgag caggtgctgc acttcttcgg
     2101 gaggctttcg gggctcgccg agccggatcg ttcgcggcgg gtggagcacc tcctgcgccg
     2161 cgtgcggatg gacgaatggc gcacgactcc ggtgcggcgc tattccaagg ggatgatgca
     2221 gcggctcggg atggcgcagg cgatcctcaa cgagccggac ctgctgatcc tcgacgagcc
     2281 gaccgacggc gtcgatccgg tcgggcggcg ggagatccgc gatctcctgc tcgagcagaa
     2341 ggagcgcggc gcgacgatct tcctcaactc gcacctgctg tcggaggtgg agcgcctgtg
     2401 cgatcgggtc gccatcctca agggcggccg gctggtgcgg gagggccacg tggaggacct
     2461 gacgcgcggt ggcgccgtga acgtctggga gatcgacgtg cagggagggg acgacgcgcg
     2521 gctcgcggcg gtgatggggc ccgcggcgct gcggccggcc gggccgcctc ccctcccggg
     2581 ggcggcggcc gtggcacgtg aagggggggc cgccgtgcgc gacggggtgc ggacgctcga
     2641 ggtctcgggg gaaaccgagg atctcaaccg ggcgatcgac gcgctgcggg ccgccgggct
     2701 gaccatcgcc gcggtgcggg cgcgccgcga aagcctcgag gacgtcttcg tgaagatcgt
     2761 ccgcgacggg gaggcggcat gaagcgcgga cgcgccgcgg ggagccgctc gtgaaccccg
     2821 gcgcgaaggt cgccgccatc gttctcgaca ccggccggga gctggcctac cggaagacgc
     2881 ttctcttcta tttcggcatc gtcacgctca cgcacctgtt cctcctgctg gcgctgcaga
     2941 ccgatgtcgc gaacggcgtc atcacctcga tccggctgtt cggcctggag gggagcgtgc
     3001 acggtttcgg cggccgcgcg gcggagggga tgggtctgga ggcgaatcaa ctggtgcgcg
     3061 gcatccagat cgccgtgacc tggacgctct atccgatcgg cgtgatgctc gccgtgtttg
     3121 cgaccgcgag cctcgtcccg cacatgctgg agaaggggac gatcgatctg ctgctgtcga
     3181 agccggtctc ccgccccgtc ctgttcgtct cccgctacct cggggcgctt ctcgtggcgg
     3241 gggcgaacct cttctatttc gtcggcgggg tcggggtgat cctggcgatc aagaccggcg
     3301 tgtggaacgg cgggttcttc ctctcgggtc tcctgatgac ggtctacttc ggcgcgctcc
     3361 tggcgttcct cgtcctcatc ggcgtgctcc tgcgctccac gaccatcggc atcatgtcgg
     3421 ccgcggcggt ctacttcgtc agcttgatcg tcgccttccc gcacgcgaac gccgactggc
     3481 cggcgctcct cacgagcaag ccggcccgct gggggacgca ggccgtcgtg gaggtcctgt
     3541 accacgccct gccccggacg ctcgagctgg ggaacatggt gacggcgctg gtgatgcagc
     3601 ggccggtcgc ctcgtggatg ccggtcgcgt ggacgatcgt ctccggcgcc ggggcgcttc
     3661 tgctcgcgat cgcctggttc cggcgctgcg atttctaggg ggagagggaa tgcgtgcgac
     3721 gagacacggc gggagcgacg ggacgtcccg gggggcggcg gggatcgcgg tggtcgtggc
     3781 gctcgcggtc gtggcggcgg tcgcgggggg cggctgctcg cggacgccca tcgacgccgc
     3841 ctccgccgtg aagcagagcg acctgagatt cctccccggc aagaccgtcg gcctggcggt
     3901 ggtcgaggtg gcgcggttcg aggacaaggc ggcgatcggc cgctggctcg acgacttcgc
     3961 gaaacaggcc ggggccgggg ggctcgaccg cgtgcgcgcg acgctcggcc cggacgcgct
     4021 cgatcgcctc gatcggatcg ccctcgccct cgtccccgcc gccggctcga cccagtacgt
     4081 cgtcctcgcc gaggggcgct tcgaggagca gacggtgaaa aacctcacgg gcggcggagg
     4141 gatcgtcacg ctggtcgaag tcggcaccgc ccccgacctc agcatcatgc cgctgccgaa
     4201 gaaccatctc gcgctcgggc cgcgcgccgt gctggagcag gtgcgggtcc tcgccgcggg
     4261 cggcggaggc gaccaggggc tcgccggggc gccggtgatg aaggtgctct cccgggtcaa
     4321 cccggcgaag cagatctggg gcgggatcga ctacagcgtg ctcgcggggc tcctcgagga
     4381 agggtcgcgc gacggcggag ggtcgccgat ggccggcttc gcacgatccc tccgagccct
     4441 ggcgttccag gggaccatcg acgacggagt gcagttcgac ctcatcggcc tcgccgacgg
     4501 ggagccggga gcgaagaccc tcgcggacgc cgcccgggga ctcgtggcga tcgcccgcgt
     4561 cggcgcttcg cagggtgcca accgcgcggc ggaaggttcg tcgggcgggg ccgccgatcc
     4621 ggcggtcgag tcggcgcagg cgtggcggcg cttcctcgac ggcgtccgga tcgaccagga
     4681 gcgggacgag atccgggtgc acgggacgct cgacggtccg atggcccgct ccttcgccgg
     4741 cgcggcgacg cgcgccgtcc ccggtgtcgg caccaactag gaccgggtcg gagctcccgg
     4801 tcgccggccg acgctcagtc ggcgggcgcc ttccagtcgc agccggcttc ctcgggcgtc
     4861 gagcgcgccg ccttgcgggc cgcctccccc agatcgatga gggcgatctt cagccggcgg
     4921 gcgagcgcca ggctcttccg gtcgcggtag aactcgccgt agtagatggt ccggatgccg
     4981 gcgttggcga tcagcttgaa gcaggaccag caggggctgg cggtggtgta cagctcggcg
     5041 ccgtcgatca tcacgccgtt cttggctgcc tgcaggatgg cgttcgcctc ggcgtggatg
     5101 gtcccgacgc agtgatcgtt ctccaggtcg tgcccgacct cgtcgcagtg cggcatgcct
     5161 cggatcgagc cgttgtagcc ggtggagagg atcgtcttgt cccgcacgat caccgccccg
     5221 acgtgcttgc ggtcgcaggt ggagcgggtc gccacctgcg tggcgatgtt catgaagtag
     5281 gtgtgccagt cgacccggtg cctcgggccg ggcggcgccg ggcggggcgc cggacgacga
     5341 gccatcgtcg gctccttacc tcgtctcgat ggtgaccgac tccatcgtca cgttcttcag
     5401 cggccggtcg gctttgccct tcgggacgag cgagatcttg tccaccacgt ccatcccctc
     5461 gaggacgcgc ccgaagaggg tgtactggtt gtcgaggaac ttggagtcct tctgcacgat
     5521 gaagaactgg caggagccgc tgttgatgtc gttgccgccg cgcgccgtcg acacggtgcc
     5581 gcgcacgtgg ctgtacttcg tgctgaactc cgccggcatc ttgcgcgggc ccgatccggt
     5641 gccgtcgttg ttcggatcgt cgtccttcga cagcgggtcc cccccctgga tcatgaagcc
     5701 gttgatcacc cggtggaagg tggtgccgtt gtagaagctg ctcttcgcca ggtccttgaa
     5761 gttggcgacc atcttcgggg ccacgtcggg caggaactcg aagacgatcg tccccatgct
     5821 ggtcttgatc accgccacgt ccttcttctt ggcctcgccg gcaggagcgc tggtcacggg
     5881 tgcactctcc ttcttgggtt cggccggcgg ggtctgggcg agcgcgactc cggccagaag
     5941 cgcggcgcac gcggtgaggg tgagcaggcg cgaggcggtc gcgcccttcg cggtcgaaga
     6001 catgcggtga tcctccctgg tgtctttggg catcgcgggc ctcgaaaacg agcgatgcgg
     6061 ggcgccgatt ctagcagagg aggcgcgcag cg
//
LOCUS       DASRIQ010000412         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3031891, whole genome shotgun sequence.
ACCESSION   DASRIQ010000412 DASRIQ010000000
VERSION     DASRIQ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3031891"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFD06_10105"
     CDS             <1..252
                     /locus_tag="VFD06_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020375332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03925.1"
                     /translation="APNCSVDVVSRRPGDKTHEVLISEEEAPYTEERDGLYVLRPGLP
                     AGRSRRFAATRLASSEAEPFSAAELRRLVNGFADGGSGR"
     gene            complement(218..1348)
                     /locus_tag="VFD06_10110"
     CDS             complement(218..1348)
                     /locus_tag="VFD06_10110"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZN03926.1"
                     /translation="MDIRMSAPWIDASDVRAVVEVLRSGQLALGPRAEEFERRFAAYV
                     GTRHAVAVNSGAAGLHLAVLAAGVRRGDDVVTTPFSFIASTNCFVSEGARPVFVDVEP
                     ETLMPAARAIDAAVTRRTRAILAVDLFGNPFDADAVAPVARRRGLALIEDACEAPGAE
                     YNGRRAGSLADAAVFGFYPNKQMTTGEGGMIVTGRKDWDALFRSLRNQGRDVHDAWIT
                     HSRVGYNYRLDELSAALGLSQLRRLDRLLEARARVADAYGRRLAGVPGVRTPRPAATT
                     TRMSWWVYVIQLDRELDRDRVMRELEARGIPSRAYFSPLHLQPAYRKRFGFRKGQFPV
                     TEDAARRSLALPFHGRMKGKEVECVCRALEQAIDRSRRRRIR"
     gene            1454..>2549
                     /locus_tag="VFD06_10115"
     CDS             1454..>2549
                     /locus_tag="VFD06_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZN03927.1"
                     /translation="MNRREWMTGVAGLTAAVGQEKNPAAQVADRTSSIRITGMKAFWV
                     NSHVFLRIDTNHGIPGWGDVKGVDPRVAKPLAESLYELIDGENPTRIEHLWQKVFRAH
                     RDIRGGPFMVHTLAAIDMALWDITGRLWGVPVYRLLGGPCRDRIRVYHTSRAIKVGPP
                     HTYEHASDPADLSKIVEIIRVAREKVGPGGSVMLDAHCLLPPATLIQLAGMLKPEELL
                     FIEEPAVPGNIEVFKRLKAAIRIPLAAGERDRTIWEVIPYLHERCLDILQPDCCHTGG
                     ITQQRKIAVLAEAYSVPIAPHCTASYLGIAASLHVAASVPNFLIHEFYPDNYGFNPKG
                     FTSMAWELDKDGYIGLPPGPGLGVDVNEKLI"
BASE COUNT          396 a          871 c          902 g          380 t
ORIGIN      
        1 gcgccgaact gctccgtcga cgtggtgagc cgccggcccg gcgacaagac ccacgaggtg
       61 ctgatctccg aggaggaggc cccgtacacc gaggagcgcg acggtctcta tgtcctccgg
      121 cccggacttc ctgccgggcg aagccggcgc ttcgccgcga cccgattggc gagcagcgag
      181 gcggagccgt tcagcgctgc cgagctgcgc cggctcgtca acggattcgc cgacggcggc
      241 tccggtcgat agcctgttcg agcgcccgac acacgcattc cacctctttt cccttcatcc
      301 ggccgtggaa gggcagggcc agcgaacgtc gggcggcgtc ctccgtcacg ggaaactggc
      361 cctttcggaa gccgaagcgc ttccggtagg cgggctggag gtgaaggggg ctgaaatacg
      421 cgcggctggg gatgccccgg gcctcgagct cgcgcatcac gcggtcccgg tccagctccc
      481 ggtcgagctg aatcacgtac acccaccagc tcatccgcgt cgtggtcgcg gcgggccgcg
      541 gcgtgcggac gcccggaacg ccggcgagcc gccggccgta cgcgtccgcc acccgggcgc
      601 gggcctccag gaggcggtcg aggcgccgga gctgcgagag tcccagcgcg gcgctcagct
      661 cgtccagccg gtagttgtac ccgacccggg aatgggtgat ccacgcgtcg tggacgtcgc
      721 ggccctggtt tcgcagcgac cggaagaggg cgtcccaatc cttccgtccg gtgacgatca
      781 tgccgccctc gccggtcgtc atctgcttgt tcgggtagaa gccgaagacg gccgcgtcgg
      841 ccaggcttcc cgcccggcgg ccgttgtact cggccccggg agcttcgcac gcgtcctcga
      901 tcagggcgag gccgcggcgg cgggcgacgg gggccacggc atcggcgtcg aacgggttcc
      961 cgaagaggtc gaccgcgagg atcgcccgcg tccggcgcgt gacggcggcg tcgatcgcgc
     1021 gcgcggcggg catgagggtt tcgggctcga cgtccacgaa gacgggtcgc gcgccttcgc
     1081 tgacgaagca gttcgtcgac gcgatgaagc tgaagggcgt ggtgaccacg tcgtcgccgc
     1141 gccggacgcc cgccgcgagc acggcgaggt ggaggccggc cgcgccggag ttgaccgcga
     1201 cggcgtgccg cgtccccacg taggcggcga agcggcgttc gaactcctcc gcccgcggtc
     1261 ccagggcgag ctgcccggag cgaagcactt cgacgacggc ccggacgtcg gacgcgtcga
     1321 tccacggcgc ggacatccgg atgtccatcg tcgaccagtc tcctgcgggg gcccgattct
     1381 gtccacaaaa tgtccgctca aggtcctttc actccaccgt aagaattgac cgggcggcgg
     1441 ctatctcctc tacatgaatc gacgcgaatg gatgacgggg gtcgcgggac tgaccgcggc
     1501 ggtggggcag gagaagaacc cggcggccca ggtcgccgat cggacctcga gcatccggat
     1561 cacggggatg aaggcgttct gggtgaattc ccacgtcttc ctccgcatcg acaccaacca
     1621 cgggatcccg ggctggggcg acgtcaaggg cgtcgatccg cgcgtggcga aaccgctcgc
     1681 cgagtctctg tacgagctga tcgacggcga gaacccgacc cggatcgagc atctctggca
     1741 gaaggtgttc cgtgcccacc gggacatccg gggcggtccg ttcatggtcc acaccctggc
     1801 agcgatcgac atggcgctct gggacatcac cgggcggctt tggggcgttc cggtctatcg
     1861 gctgctgggc ggcccctgcc gcgaccgcat ccgcgtctat cacacgtccc gggcgatcaa
     1921 ggtcgggccg ccccacacgt acgagcacgc gagcgatccg gcggacctgt cgaagatcgt
     1981 cgagatcatc agggtcgccc gggagaaggt cggccccggc ggctcggtca tgctcgacgc
     2041 ccactgcctg ctgccgccgg cgacgctgat ccagctggcc gggatgctga agcccgagga
     2101 gcttctgttc atcgaggagc ccgcggttcc ggggaacatc gaggtcttca agcggctgaa
     2161 ggcggcgatc cgcatcccgc ttgcggccgg cgagcgggac cggacgatct gggaggtgat
     2221 cccgtacctg cacgagcggt gcctggacat cctgcagccg gactgctgcc acacgggcgg
     2281 gatcacccag cagcggaaga ttgcggtgct cgccgaggcg tacagcgttc cgatcgctcc
     2341 ccactgcacg gcgagctatc tcggcatcgc cgcgagcctg cacgtcgcgg cgtcggtccc
     2401 gaacttcctc atccacgagt tctacccgga caattacggc ttcaatccca aagggttcac
     2461 gtcgatggcg tgggagctgg acaaggacgg gtatatcggg ctgccgcccg gcccgggcct
     2521 cggagtggac gtgaacgaaa agctgatcg
//
LOCUS       DASRIQ010000413         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_699592, whole genome shotgun sequence.
ACCESSION   DASRIQ010000413 DASRIQ010000000
VERSION     DASRIQ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699592"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2007)
                     /locus_tag="VFD06_10120"
     CDS             complement(<1..2007)
                     /locus_tag="VFD06_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HZN03928.1"
                     /translation="MPDWRRYVRERLSLPRLRPEREAETVEDLAQQLDDLYRAALERG
                     ATEEEAEAQARREIGDWDGLAHDITLSETRHRHPLDQRAIDHLETGRRPAGAVFRIAA
                     QCVADALHGVRLLRQKPGFTVAVLVTLALGIGANTAVFTILDTVLLRPLPYAEPERLV
                     RIWESNPGRGWPQFGVSQPNFLDWREQGGDAFERLSASTSRPHNLLVGGEAERLPGLA
                     ITHDFLPMLEVRPLVGREFLPEEDAPGRGERVVLLSRGLWQRRFGGDPDVVGRAITLN
                     DVPHTVVGVLPEFHWGRYEVFVPLRPDPNEPRGDHRLSVYGRLKPGVGLDQARTALEG
                     VAGRLARQYPDSNDGWTVTLSSFFDWTVPRDSRRPLYVLLAAVALVLLIACANVAGLL
                     LARAAGRRREIAVRAALGASRARLVRQLLAEAMLLAVLGGGLGLLAAQWGLTTLKAAA
                     DTAVPRADEIALDHRVLLFTLGMTVLTGLLFGLAPALQATRVDLQGALKDGATGDGPM
                     RQRARSVLVVGQVALSLVLLVGVGLLLRSLVALVDTPPGFDPRNLLTASINLPESRYP
                     TPKEFAAFHQRLLQRLRGLPGIDAASITSGLPMDGNGTMMEVYLDEAPAAPDGPAPSA
                     QWRLVSPGYFRTMGIPLRAGRDLEERDLAPDTFDLRGAVISETMA"
     gene            complement(2000..2356)
                     /locus_tag="VFD06_10125"
     CDS             complement(2000..2356)
                     /locus_tag="VFD06_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN03929.1"
                     /translation="MSQALEGTMFSRELKKGSTELLILSLLDDRPRHGYDLGKRIEER
                     SGGRLQLRIASLYPMLVRLESRGLIRGRWVERPGERRRRFWRLTSEGRRVLARERATW
                     KEFMAAVNAVVGGSHA"
     gene            2557..4329
                     /locus_tag="VFD06_10130"
     CDS             2557..4329
                     /locus_tag="VFD06_10130"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZN03930.1"
                     /translation="MSSLRTLAGGTLLLAALLSASPACRSGSSITNRLEEILGGISER
                     GGRKEASAGEIREALQHQLRVAPVPAAGSNRPSSPAFPTRQTLVEFYARRSQRLVWTS
                     ESGAILPVADTLLEALERAGEHGLDPAEYAVASLERMRKQVGAARGGASAVTRWADFD
                     LLLTTAFFRYASDLSSGRVHPDEIRSEWLTEPPELDLPGALDKALAEGRLERLLEGLP
                     PPHPGYERLREALEDLRKIEAAGGWKSIPSGPALKKGARGQRVALLRERLGVRGGILR
                     PEAKRAGPGAVFDVATQDAVKDFQSRHGIEPDGIVGEGTLAALNVPVARRVRQVELNL
                     ERWRWMPRRLGDVHVEVNIPGFELALVRNGKADFRSRIVAGSAFTPTPIFSDRIVAVV
                     ANPPWNVPRELAVREYLPELKHDPKLFMRRGIRIYKDTGKKDADEEAEEIDPTSVSWR
                     RVDEDEFDYRLRQDPGPENALGRMKLHLTNDFQIYLHDTPARAAFTEASRDLSHGCIR
                     VEQARALADRLLGDDAPRLEEALESLDEQSLPVRPPVPIQILYLTAWVDEEGTLRFGP
                     DVYEFDAPQQAALDRIARAARRAP"
BASE COUNT          663 a         1580 c         1550 g          586 t
ORIGIN      
        1 cgccatcgtc tcgctgatca cggcgccccg caggtcgaag gtgtccggcg ccaggtcccg
       61 ctcctccagg tcgcgcccgg cgcgcagcgg aatccccatg gtgcggaagt acccggggga
      121 gaccagccgc cactgcgccg acggagcggg gccgtccggc gccgcgggag cctcgtccag
      181 gtagacctcc atcatggtgc cgttgccgtc catcggcaga ccgctggtga tcgacgcggc
      241 gtcgatcccg ggaagccctc gcagacgctg caggagacgc tgatggaacg cggcgaactc
      301 cttcggagtc gggtagcgcg actccggcag gttgatgctc gcggtcagga ggttcctcgg
      361 gtcgaaaccg ggcggcgtgt cgacgagcgc caccaggctt ctcagcagca gcccgacgcc
      421 gaccaggagg acgagcgaga gcgccacctg accgaccacc agcacgctgc gcgcccgctg
      481 ccgcatcggg ccgtcgcccg tcgctccgtc cttgagcgcc ccctggaggt ccacccgcgt
      541 cgcctgcagc gctggcgcga ggccgaagag gagcccggtg agcacggtca tccccagcgt
      601 gaacagcagc acgcggtgat cgagggcgat ctcgtcggcg cgcggcacgg ccgtgtcggc
      661 cgcggccttc agcgttgtca ggccccactg ggcggccagg aggcccagcc cgcctcccag
      721 caccgccagg agcatcgcct cggcgaggag ctgacggaca aggcgcgccc ggctcgcgcc
      781 gagcgccgcg cggacggcga tctccctgcg gcggccggcg gcacgagcca ggaggaggcc
      841 ggcgacgttg gcgcaggcga tgaggagcac gagcgccacc gccgcgagca gcacgtacag
      901 gggacggcgc gagtcccggg gcaccgtcca gtcgaagaag ctgctcaacg tgacggtcca
      961 gccgtcgttc gagtcgggat actgccgcgc caggcgcccg gcgacgccct cgagcgcggt
     1021 ccgcgcctga tccaggccga cccccggctt caagcggccg tacaccgaga gccggtggtc
     1081 gccgcgcggc tcgttcggat ccggccgcag cggcacgaac acttcgtacc ggccccagtg
     1141 gaattccggg aggacaccga ccacggtgtg cgggacgtca ttgagcgtga tggcgcgccc
     1201 gacgacgtcc ggatctccgc cgaagcggcg ctgccacagg ccgcggctga gcagcacgac
     1261 gcgctccccg cgtccgggag cgtcctcctc cgggaggaac tcccggccga cgagcggccg
     1321 gacctcgagc atcggaagga agtcgtgcgt gatcgccagt cccggcaggc gctcggcttc
     1381 gcctccgacc agcaggttgt gcggccggct cgtcgacgcc gagagccgct cgaacgcgtc
     1441 ccctccctgc tcgcgccagt cgaggaagtt cggctgcgac acaccgaact gcggccagcc
     1501 gcgtcccggg ttgctctccc agatgcgcac cagccgctcc ggctcggcat acggcagagg
     1561 gcgcagcagc acggtgtcga ggatggtgaa caccgcggtg ttggcgccga tgccgagcgc
     1621 gagcgtgacc aggaccgcga ccgtgaagcc gggcttctga cgaagcagcc ggaccccgtg
     1681 gagcgcgtcc gccacgcact gcgcggcgat gcggaagacc gccccggccg gacgccggcc
     1741 ggtctcgagg tgatcgatgg cccgctgatc gaggggatga cggtggcgcg tctccgagag
     1801 cgtgatgtcg tgcgccaggc catcccagtc cccgatctcg cgccgggcct gcgcctccgc
     1861 ttcctcctcg gtggcgccgc gctccagggc ggcccggtag agatcgtcga gctgctgcgc
     1921 caggtcctcc acggtctcgg cctcccgctc tggccgcaga cgcggaaggc tcagccgctc
     1981 acggacgtac cgccgccagt caggcatggc tgcctccgac aacggcgttc accgcggcca
     2041 tgaactcttt ccaggtggcc cgctcgcggg ccagcacgcg ccggccctcg gaggtcaacc
     2101 gccagaagcg tcggcgccgc tcccccggcc gctccaccca gcgtccccgg atgagcccgc
     2161 ggctctcgag gcggaccagc atcggataga gcgaggcgat tcgcagctgc agccggccgc
     2221 cggagcgctc ttcgatacgc ttgccgaggt cgtagccgtg ccgcgggcgg tcgtcgagca
     2281 gcgacaggat cagcagctcg gtgctcccct tcttcagttc ccggctgaac atcgtcccct
     2341 ccagcgcttg cgacacgaca tatcgcttag aggcatatac tcggatcgtg gacggctgtc
     2401 aagggcgaac cggagaggtc gccggccgcg cgacgctcga cgtctgggac gggagaatcg
     2461 aggacgctcc ggccctcgcg gtgggaacgc cctgacatcc ccctgaggat tcccctgacc
     2521 aatccggggg actccgttcg atagtattga cgcctcatgt cttcgctacg gaccctggcg
     2581 ggcggcaccc tccttcttgc ggcactgctc tccgcgagcc cggcctgccg gtcgggctcg
     2641 tcgatcacca accggctgga ggagatcctc ggcgggatca gcgagcgcgg cgggcgcaag
     2701 gaggcgtccg ccggcgagat ccgcgaggcg cttcaacacc agctgcgggt cgcgccggtg
     2761 ccggcggcag ggagcaaccg gccgtccagc cccgccttcc cgacccgcca gacgctcgtc
     2821 gagttctacg cgcgccggag tcagcgcctg gtctggacga gcgagtcggg ggcgatcctc
     2881 ccggtcgccg acacgctcct ggaggcgctc gagcgggccg gcgagcacgg gctggatccg
     2941 gcggagtacg ctgtggcctc gctcgagcgg atgcgcaagc aggtcggcgc ggcgcgcggc
     3001 ggcgcatcgg ccgtcacccg ctgggccgat ttcgatctgc tcctgaccac cgcgttcttc
     3061 cgctatgcct ccgacctgtc ctccggccgc gtccatcccg acgagatccg cagcgagtgg
     3121 ctgaccgagc cgcccgaact ggatctgccg ggcgccctcg acaaggcgct cgcggaagga
     3181 cggctcgaga ggctcctcga ggggcttcct ccccctcacc ccggctacga gcgcctgagg
     3241 gaggccctcg aggatctccg gaagatcgaa gcggccggag ggtggaagtc gatcccgtcc
     3301 gggccggctc tgaagaaagg agcgcgcggg cagcgcgtgg cgctcctccg ggagcgcctc
     3361 ggggtgcgag gcggcatcct gcggcccgaa gcgaagcgcg cgggcccagg cgccgtcttc
     3421 gacgtcgcga ctcaggatgc ggtcaaggac ttccagagtc gccacggaat cgaaccggac
     3481 ggcatcgtcg gggaggggac gctcgcggcg ttgaacgtcc cggtggcccg acgcgtgcgc
     3541 caggtcgagc tgaacctcga gcgctggcgc tggatgccgc gccgcctcgg cgacgttcac
     3601 gtcgaggtga acatccccgg gttcgagctg gcgctcgtca ggaacgggaa ggcggacttc
     3661 cggagccgga tcgtcgcagg aagcgccttc actccgaccc cgatcttcag cgacaggatc
     3721 gtcgcggtcg tggccaaccc gccctggaac gtcccgagag agctggcggt gcgggagtac
     3781 ctgcccgagc tcaagcacga cccgaagctg ttcatgcggc gcggcatccg gatctacaag
     3841 gacacgggca agaaggacgc ggacgaggag gccgaagaga tcgatccgac gagcgtctcg
     3901 tggcgccgcg tggacgagga cgagttcgac taccgcctgc gccaggaccc ggggccggag
     3961 aacgccctcg ggcggatgaa actccatctg accaatgatt tccagatcta cctgcacgac
     4021 acgccggcgc gcgcggcgtt caccgaggcg agccgagacc tgagccacgg atgcatccgc
     4081 gtcgagcagg cgcgcgcgct ggccgaccgg ctcctggggg acgacgcgcc gcggctggag
     4141 gaggcgctcg aatcgctcga cgagcagtcg cttccggtgc gccctccggt gccgatccag
     4201 atcctgtacc tgaccgcctg ggtggacgag gaggggaccc tgcgcttcgg gcctgacgtt
     4261 tacgaattcg atgccccgca gcaggcggcc ctggatcgca tcgcgcgcgc cgcccgccgc
     4321 gctccctgaa tctaggcgcc cggccaggtc attctggccg tgcatcgcct ccgttcccg
//
LOCUS       DASRIQ010000414         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_942795, whole genome shotgun sequence.
ACCESSION   DASRIQ010000414 DASRIQ010000000
VERSION     DASRIQ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942795"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..629
                     /locus_tag="VFD06_10135"
     CDS             48..629
                     /locus_tag="VFD06_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03931.1"
                     /translation="MKSRLTAVLPALLLAFALPAAASVVPYLGLEDLGREGQVIVVGK
                     VSEVEPGLSDDGRIIVTRVTVRIERALKGGPREMVTLEIPGGTLGGTTLVASGAPRFA
                     TGERVVLFLEPQAEGRFGVAGWNQGKFTVRRDPHTGRDLVTQPGGGSLYVDRNGRPAP
                     RDPRLAGPRLLADFLRDIEAIVAASEPGKGDAR"
     gene            731..>2683
                     /locus_tag="VFD06_10140"
     CDS             731..>2683
                     /locus_tag="VFD06_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03932.1"
                     /translation="MTGAARAYIQTNLVDSGAGDVGLRQPGTYTLRLEQDGAAGITNG
                     TDLTALRDAMTRWTSVLTSNASISEGTLFDLASPIDASQGLGTPGNRLIFAQTDTQNR
                     IGSAIAVAFYAFGADGNISDCDIVMNERLFTFSTATPSNPNQVLGQSTYDIGEIATHE
                     MGHCLGLDHSPVAGRFSASTGLQVSGFSSADFAFQATMYPYGTRTIQGRSLSEDDIAG
                     ASFVYPNATLQSTRGTLSGRVLDGGSFAPVKGAHVVAVTTAAPDVPVIGVVSDVQAGG
                     AGGEFNIVGLAPGTYYLRLEPLVGTSNPFTSGNTHFNSFTTSFPPEYYDGAGESGFDD
                     PSARVPLTVTAGQVTGNLTIFTNVGAPDPNEPNNSRASATAAGCEVQKTGAIVPLNDV
                     DYYSVATTAATRLSASVSAVQQGSSLDAVLGVFDAGGALLAFDDNDVTLDPALAIDLP
                     DPGTYYVAIASYNDAALAGSGGQTVGSYALNLTCTLPPVQAGTCPGKVLYAGNGVTGT
                     LVAISDADGDLAFDGRSTLYPAVGTGQGQLVTRRDGTTVVARQDGVLFGATDADGDFH
                     AESVLFGDNGFLDGGAIAILRRGGEERFFVGGTFSGGGTVTDWLDADGDLLPDQEIVF
                     TTVPDSVFVLSIDEAGTVYVLDYLANE"
BASE COUNT          388 a          984 c          924 g          387 t
ORIGIN      
        1 cctccgccgt gcaggatccg ccccggatcc gctcccggag accccgcttg aagtcgcgcc
       61 tgacggccgt tctgcctgcc ctcctgctcg ccttcgccct tcccgccgcc gcgtcggtcg
      121 tcccgtacct ggggctcgag gacctcggtc gcgaggggca ggtgatcgtc gtcgggaagg
      181 tgagcgaggt cgaaccgggc ctgagcgacg acggccggat catcgtcacc cgcgtgaccg
      241 tccgcatcga gcgggcgctg aagggggggc cgcgggagat ggtgaccctc gagattccgg
      301 gtgggacgct ggggggcacg accctcgtcg cctccggggc gccgcgcttc gcgacggggg
      361 agcgggtggt gctcttcctc gagccgcagg cggagggacg gttcggcgtc gccggctgga
      421 accagggaaa gttcaccgtc cgacgtgacc cgcacaccgg gcgcgatctc gtcacccagc
      481 cggggggcgg ctcgctctac gtggatcgaa acggccgtcc ggcgccgcgg gatccgcgcc
      541 tggccgggcc gcggctgctg gccgacttcc tgcgcgacat cgaggccatc gtcgccgcct
      601 ccgaacccgg gaagggggac gcccggtgag ccgcggagcg cgacccctgg cgtccgctcg
      661 gagcggggaa gtcggccggt cgggacgctt ccgccgcgtc gcgctgggat gcgcggccgc
      721 gggattcctg gtgaccggag cggcgcgcgc ctacatccag accaacctgg tggattccgg
      781 cgccggagac gtcggtctcc gccagccggg gacgtacacc cttcgcctcg agcaggacgg
      841 cgcggccggc atcaccaatg ggaccgacct gacggcgctc cgcgacgcca tgacccgctg
      901 gaccagcgtt ctcacctcga acgccagcat cagcgaagga acgctgttcg acctggccag
      961 cccgatcgac gccagccagg gactgggaac tccggggaac cggctgatct tcgcccagac
     1021 cgatacgcag aaccggatcg gcagcgccat cgccgtcgcc ttctatgcct tcggagccga
     1081 cgggaacatc agcgactgcg acatcgtcat gaacgagcgg ctgttcacct tcagcaccgc
     1141 caccccgtcg aacccgaacc aggttctcgg ccagtccacg tacgacatcg gcgaaatcgc
     1201 gacccacgag atgggtcact gtctcggcct tgaccacagc cccgtcgccg ggcgcttctc
     1261 cgcctcgacc gggctgcagg tgtcgggatt ctcgagcgcc gatttcgcct tccaggcgac
     1321 gatgtatccg tacggaacgc gcaccatcca ggggcggagc ctgtcggagg acgacatcgc
     1381 cggggcttcg ttcgtctacc cgaacgcgac gctccagtcg acccgcggca ccctgtcggg
     1441 ccgcgtcctc gacggcggca gcttcgcgcc ggtgaagggg gcgcacgtgg tggcggtgac
     1501 caccgccgcc cccgacgtgc cggtcatcgg ggtggtctcg gacgtgcagg ccggcggagc
     1561 gggaggggag ttcaacatcg tcggtctcgc gccggggacc tattacctgc gcctcgagcc
     1621 gctcgtgggc accagcaacc cgttcacctc cgggaacacc cacttcaaca gcttcacgac
     1681 ctcgttcccg ccggagtact acgatggcgc gggggagtcg gggttcgacg acccgtcggc
     1741 gcgcgtcccg ctgaccgtca ccgccgggca ggtgaccggc aacctgacca tcttcacgaa
     1801 cgtcggcgcc cccgatccca acgagccgaa caacagccgc gcctccgcca ctgccgccgg
     1861 ctgcgaggtg cagaagaccg gcgccatcgt cccgctcaac gacgtggact attactcggt
     1921 ggccaccacc gccgccaccc gcctctccgc gagcgtgagc gcggtgcagc agggctcttc
     1981 cctcgacgcg gtgctcgggg tgttcgacgc cggtggggcg ctcctggcgt tcgacgacaa
     2041 cgacgtcacc ctcgatccgg cgctggcgat cgacctgccc gaccccggga cctattacgt
     2101 cgcgatcgcc tcgtacaacg acgcggcgct cgccggcagc ggcgggcaga cggtcggctc
     2161 gtacgccctg aacctcacgt gcaccctccc gccggtccag gccggcacct gcccggggaa
     2221 ggtcctctac gccggcaacg gagtcaccgg gacgctcgtg gcgatctccg acgccgacgg
     2281 cgatctcgcc ttcgacggcc ggtccacgct gtacccggcg gtgggcaccg gccaggggca
     2341 gctcgtgacc cggcgcgacg gcacgacggt cgtggcgcgg caggacgggg tgctcttcgg
     2401 cgcgaccgac gccgacggcg acttccacgc cgagtcggtc ctcttcggcg acaacggctt
     2461 cctcgacggc ggggcgatcg cgatcctgcg tcgcggcggg gaggagcgct tcttcgtcgg
     2521 aggaacgttc tcgggcggcg gcacggtgac ggactggctc gacgccgacg gcgacctcct
     2581 gccggaccag gagatcgtct tcacgaccgt cccggattcg gtcttcgtcc tgtcgatcga
     2641 cgaggccggc accgtctacg tgctcgacta cctggcgaac gag
//
LOCUS       DASRIQ010000415         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_943073, whole genome shotgun sequence.
ACCESSION   DASRIQ010000415 DASRIQ010000000
VERSION     DASRIQ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943073"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1181)
                     /locus_tag="VFD06_10145"
     CDS             complement(120..1181)
                     /locus_tag="VFD06_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZN03933.1"
                     /translation="MPDPEDGAPGRDVSDRYARQVLFQPLGEKGQQALGRATALLVGC
                     GALGTHLADLLARAGVGRLRIVDRDVVERSNLHRQSLFDERDVAEGLPKAAAAARRLA
                     DVNPEIAVEAVAAHLDAGNIGRHADGVDLLLDGTDNFETRFLLNDLALQRSIPWVYAA
                     CIGAYGMTMTIVPGTTPCLRCLVRDPPAPGTVDTCDTAGILAPAAALASALAAGEAIK
                     ILSGAIEAISRDLVAFDLWSNTTQRLRMERASMSPGCPACDQGRYEFLAAGAGTRAVT
                     LCGRNAVQVRPGPGKKADLDLLARRWESIAKVTRNPYLLRMDVEECTLAVFADGRAII
                     SGTSDEGRARALYDRYVGG"
     gene            complement(1168..2649)
                     /locus_tag="VFD06_10150"
     CDS             complement(1168..2649)
                     /locus_tag="VFD06_10150"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HZN03934.1"
                     /translation="MTSRLPRRPLCIEVVAQRTMTAALEAYRDAHPRADLVEFRLDLV
                     RDLDLDRLIAARGKPKLMTVRSRPQGGEAAPADRPALLRRLCRAPVAWVDVEPDDFGL
                     AEVRRPGGPKRLLSWHDLHTTPLDLEDRLARLLAERRAALVKLVTFAEVAGDLLRVRD
                     LLRRAPAGRVLSFCMGPKGVASRILGPSWGSVGVFAPRRGAPASAPGQVPLEDLVETY
                     RLDTLRPSTGLLGVLGSPVGHSLSPAMHNAALEALGLDLRYLPFEASTVAEFLPVLSE
                     LRLRGLSVTLPFKEAIVPHLDRVDAGARRLGAVNTVVKIWNRLEGSNTDAAASLVPLR
                     GFVRLKDARIGVLGAGGAARALVPGLVRRGARVTIFSRTPGRGRDLARASGARALPWG
                     RARGFRCDLLVNATPVGMGVRAKALPVPIAWVSARRVYDLIYTPAETALVRRARARGI
                     PAKGGLEMFLAQGAAQFRLFTGQPAPRAVMRRAAQEALVDARS"
     gene            complement(2646..>3085)
                     /locus_tag="VFD06_10155"
     CDS             complement(2646..>3085)
                     /locus_tag="VFD06_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZN03935.1"
                     /translation="EEVWEQTGGRIDWLVLTLGTCGTLVGVARRLKERHPSLKVVSVE
                     PQEQHLQQGLRNMSESKVPQIMDWSLVDERLTVADADAARLTLALARQEGILAGISSG
                     TAVYAALEVARRAGRGDIVTLLPDRGEKYLSTTLFPPGPDPAA"
BASE COUNT          440 a         1136 c         1130 g          379 t
ORIGIN      
        1 acgacaggga cagaagggcc aggaggagcg ccgcgcgggc gcgggatgaa acgggagtgt
       61 ccatgcgcgc cggatcatac gccatgcggc gcggcgcgcg cggggtctgc cctgctggct
      121 cagcctccca cgtagcgatc gtagagggcg cgggcccgcc cctcgtcgga ggtccccgag
      181 atgatggcgc ggccgtccgc gaacaccgcg agcgtgcact cctcgacgtc catcctgagg
      241 aggtaggggt tccgggtcac cttggcgatc gactcccagc gccgcgcgag gaggtcgagg
      301 tcagcttttt tccccggacc ggggcgcact tgcacggcgt ttcgtccgca gagcgtcacc
      361 gcccgcgtgc cggcgccggc cgcgaggaat tcgtaccggc cctgatcgca ggcggggcag
      421 ccgggcgaca tgctcgcgcg ctccatgcgc agccgctgcg tggtgttgga ccagagatcg
      481 aaagcgacga ggtcgcggct gatcgcctcg atcgcccccg agaggatctt gatcgcctct
      541 cccgccgcga gggccgacgc cagcgccgcc gccggcgcga ggatgccggc ggtgtcgcag
      601 gtgtccaccg ttcccggagc ggggggatcg cgcacgaggc agcgcaggca gggggtggtc
      661 cccggcacga tggtcatcgt catgccgtag gcgccgatac aggcggcgta gacccaggga
      721 atggaacgct gaagcgcgag gtcgttcagg aggaagcgcg tctcgaagtt gtcggtgccg
      781 tcgagcagga gatcgacgcc gtcggcgtgg cgcccgatgt tgcccgcgtc gaggtgcgcc
      841 gccactgcct cgacggcgat ctccgggttg acgtccgcga gccggcgcgc cgcggccgcc
      901 gctttcggca gcccttcggc cacgtcgcgc tcgtcgaaga gggactgacg gtgcaggttc
      961 gatcgctcga cgacgtcccg atcgacgatc cgcaggcggc cgaccccggc gcgcgccagg
     1021 aggtcggcca ggtgcgtgcc gagggcgccg cacccgacga gcagggcggt cgcccggccg
     1081 agcgcctgct gccccttctc cccgagcggc tggaagagga cttgccgggc gtagcggtcc
     1141 gaaacgtcgc gtccgggcgc gccgtcttca ggatcgggca tcgacgagcg cctcctgcgc
     1201 ggcgcggcgc atgacggcgc gcggcgccgg ctgacccgtg aacagccgga actgggccgc
     1261 cccctgcgcc aggaacatct cgagccctcc cttcgccgga atgccgcggg cgcgcgccct
     1321 ccgcacgagc gcggtctcgg ccggcgtgta gatcagatcg taaaccctgc gggcgctcac
     1381 ccacgcgatc ggaaccggca gcgctttggc gcgcaccccc atgccgaccg gcgtggcgtt
     1441 caccaggaga tcgcagcgga acccccgggc ccgcccccag ggcagcgccc gcgccccgga
     1501 cgcccgggcc agatcgcggc cgcgccccgg cgtgcggctg aagatggtga cgcgggcgcc
     1561 gcggcgcacg agccccggga cgagcgcccg cgcggcaccc cccgccccga gcaccccgat
     1621 ccgcgcgtcc ttcagcctca cgaatccgcg cagcggcacg agcgacgcgg ccgcgtcggt
     1681 gttcgagcct tcgagtcggt tccagatctt gacgacggtg ttgaccgctc cgagccgccg
     1741 cgccccggcg tcgacccgat cgaggtgcgg cacgatcgcc tccttgaagg ggagggtgac
     1801 gctcagtccc cggaggcgca gctcgctgag caccgggagg aactcggcga ccgtcgaggc
     1861 ctcgaacggc aggtaacgga ggtcgagccc gagcgcctcg agggcggcgt tgtgcatcgc
     1921 cggcgacagc gagtgcccga ccgggcttcc cagcacgccg aggaggccgg tcgacggcct
     1981 gagggtgtcg agacggtacg tctccacgag gtcctcgagc ggcacctgcc ccggcgcgga
     2041 ggccggcgct ccgcgccgcg gtgcaaagac ccccacgctc ccccacgacg gcccgagaat
     2101 gcggctcgcc acccccttcg gccccatgca gaaagagagc acccgccccg ccggagcgcg
     2161 gcgcaggaga tcccgcacgc gcaggagatc cccggccacc tccgcgaagg tgacgagctt
     2221 cacgagggcg gcgcgccgct cggcgagcag ccgcgccagc cggtcctcca ggtcgagcgg
     2281 tgtcgtgtgc aggtcgtgcc aggagagcag gcgcttgggt cccccggggc gccgcacctc
     2341 cgccaggccg aagtcgtccg gctcgacgtc cacccaggcg accggcgccc ggcacaggcg
     2401 gcgcagcagg gcgggacgat cggccggggc cgcctcccct ccctggggac ggctcctcac
     2461 ggtcatgagc ttcggcttgc cgcgcgccgc gatcagccga tcgagatcga gatcgcgcac
     2521 gagatcgagc cggaactcga cgagatcggc acgcgggtgg gcatcccggt aggcctccag
     2581 ggccgcggtc atggtccgtt gcgcgacaac ctcgatgcag agcggccggc gcggcaggcg
     2641 ggaggtcacg ccgcggggtc gggcccggga gggaacaggg tcgtgctcag gtacttctcg
     2701 ccccgatccg gcagaagcgt cacgatgtcg ccgcggccgg cgcgccgggc cacctcgagc
     2761 gcggcgtaga ccgccgtccc ggacgagatg ccggcgagaa tcccttcctg ccgcgccagc
     2821 gcgagcgtca gccgggcggc gtccgcgtcg gcgacggtga gccgctcgtc caccaggctc
     2881 cagtccataa tctgcggcac cttcgactcg ctcatgttcc gcaggccctg ctgcagatgc
     2941 tgctcctggg gctcgacgga gaccaccttg agggagggat gccgctcctt gagccgccgg
     3001 gcgaccccga cgagcgtgcc gcaggtcccg agcgtcagga ccagccagtc gatgcggccg
     3061 cccgtctgct cccacacctc ctccg
//
LOCUS       DASRIQ010000416         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2245222, whole genome shotgun sequence.
ACCESSION   DASRIQ010000416 DASRIQ010000000
VERSION     DASRIQ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245222"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1492
                     /locus_tag="VFD06_10160"
     CDS             116..1492
                     /locus_tag="VFD06_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN03936.1"
                     /translation="MASNGFTRETLQQYADSERTPFEAALKELVEIPTVSVDPARKAD
                     VARGAEYAASLLRRFGAETRIVSTPGHPIVVGKFQRNSAWPTVTLYNHLDVQPAEMED
                     GWSAAPFRFEANDGRYFGRGTTDDKGPALSALWGARYALQHGARINLQVLWELEEEIG
                     SPNFESTIRKEREALKTDTAIVSDTVWVSRNRPACPAGLRGLQGFRFTLEFGATDQHS
                     GVTGGAARNPVTEICALIAECVDARTGKIKIPGFYKDVVPPTKQELADMKRSGFTTAG
                     FKKDHHFKSLRTDDPLEIMKRVWCLPTFEVHGIAGGYQGPGVKTVVPPRATAIVSCRL
                     VPEMKETKVIKMVRDFLKKKNPDVKVEVEHGLPAYRGRTTGPHADAIRAAMKFAFGRE
                     PSFVREGGSIGAVLSMERVLKAPVYFLGLSLPEHGYHAPNENYDWSQASGGMAAFADY
                     FRRVGEMA"
     gene            1549..>2197
                     /locus_tag="VFD06_10165"
     CDS             1549..>2197
                     /locus_tag="VFD06_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HZN03937.1"
                     /translation="MSALPILIGAVLVLTLGYRYYSAFLAARVMVLDDARVTPAHRLR
                     DGHNYLPTPKWVLFGHHFAAIAGAGPLIGPVLAAQFGWAPGLLWLLLGVVFGGAVHDF
                     IILTASVRRDGRSLAEIARTEINPMAGVTAMIAILFIVVIALAGLGFAVVNALEHSPW
                     GTFTIAVTIPLALFMGLYMYRVRPGAYVSGTVVGVVGLIAAVIAGRLIPGSALAPF"
BASE COUNT          340 a          765 c          740 g          352 t
ORIGIN      
        1 gccgacaggc ggcgtgcgcg tccggccgtg cctccggagg tgacaacggc ctcccggcgc
       61 cgagattgac gcccatacgc cccatggcta tgctccgccg gtcacaggag gaccgatggc
      121 ctcgaacgga ttcactcgag aaacgctgca gcaatacgcg gattcggaac ggactccttt
      181 cgaagcggcg ctcaaagagc tggtcgagat tccgaccgtc tcggtcgatc ccgcacggaa
      241 ggcggacgtg gcgcggggcg ccgagtacgc cgcaagcctt ctgcgccgct tcggagccga
      301 gacgcgcatc gtctcgaccc ccggccaccc gatcgtcgtc gggaagttcc agcgcaactc
      361 cgcctggccg accgtcaccc tttacaacca cctcgacgtg cagccggcgg agatggagga
      421 cggctggagc gcggcgccgt tcaggttcga ggcgaacgac gggcgctact tcggccgcgg
      481 gacgaccgac gacaaggggc cggcgctgtc ggccctgtgg ggcgcccgct atgcgctgca
      541 gcacggggcg cgcatcaacc tccaggtgct gtgggagctg gaggaggaga tcggcagccc
      601 caacttcgag tcgacgatcc gcaaggagcg cgaggcgctc aagaccgaca ccgccatcgt
      661 ctccgatacg gtgtgggtgt cgcggaaccg gccggcctgc ccggcggggc tgcgaggctt
      721 gcaggggttc cggttcaccc tcgagttcgg cgccaccgat cagcattccg gggtcaccgg
      781 cggcgcggcc cgcaacccgg tcaccgagat ctgcgccctc atcgccgagt gcgtggacgc
      841 ccgcacgggg aagatcaaaa tccccgggtt ctacaaggac gtcgtgccac ccacgaaaca
      901 ggagctggcc gacatgaagc gctccggctt caccaccgca ggcttcaaga aggaccatca
      961 cttcaagtcg ctcaggacag acgacccgct cgagatcatg aagcgggtct ggtgcctccc
     1021 gaccttcgag gtgcacggca ttgccggcgg ctaccagggg cccggggtca agacggtggt
     1081 cccgccgcgc gccacggcca tcgtctcctg ccggctggtt ccggagatga aggagaccaa
     1141 ggtcatcaag atggtgcgcg acttcctgaa gaagaagaat ccggacgtca aggtggaggt
     1201 ggagcatggc ctgccggcct accgcggacg taccaccggc ccgcacgccg acgccatccg
     1261 tgccgcgatg aagttcgcgt tcggccgcga gccatcgttc gtacgcgagg ggggctcgat
     1321 cggcgcggtc ctgtcgatgg agcgggtgct caaggcgccg gtctacttcc tcgggctgtc
     1381 cctgccggaa cacggctacc atgcgccgaa cgagaactac gactggtctc aggcctcggg
     1441 cgggatggca gccttcgccg attacttccg ccgcgtcggc gagatggcct gacgatcccc
     1501 gcggccggaa gaggcggagc gctccgatga tccggaggcc gggctgacgt gagcgccctg
     1561 ccgatcctca tcggcgccgt cctcgtcctc accctcggct accggtacta ctccgccttc
     1621 ctggcggcgc gcgtcatggt cctggacgac gcgcgcgtca cccccgccca ccgcctgcgg
     1681 gacgggcaca actacctgcc gactccgaag tgggtcctgt tcggtcatca cttcgcggcc
     1741 atcgccggcg ccggtcccct gatcgggccg gtcctggccg cccagttcgg ctgggccccg
     1801 ggccttctgt ggctgctgct gggggtggtc ttcggcggcg ccgtgcacga cttcatcatc
     1861 ctcacggcgt cggtgcgacg tgatggacgg tcgctggcgg agatcgccag gaccgagatc
     1921 aaccccatgg ccggggtcac ggccatgatc gccatcctgt tcatcgtggt gatcgccctc
     1981 gccgggctgg ggtttgcggt cgtcaacgcg ctcgagcatt ccccgtgggg gacgttcacc
     2041 atcgcggtga ccattcccct cgccctgttc atgggtctct acatgtacag ggtccggccg
     2101 ggcgcctatg tcagcgggac ggtcgtggga gtcgtcgggc tcatcgcagc cgtgatcgcc
     2161 ggccgtctca tccccggctc ggccctcgcc cccttct
//
LOCUS       DASRIQ010000417         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_174937, whole genome shotgun sequence.
ACCESSION   DASRIQ010000417 DASRIQ010000000
VERSION     DASRIQ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174937"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="VFD06_10170"
     CDS             <1..162
                     /locus_tag="VFD06_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018071480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HZN03938.1"
                     /translation="ALFVDKIPKGAKPGDLPVEQPTKFELVINLKTAKALGLTIPPSL
                     LARADQVIE"
     gene            159..1136
                     /locus_tag="VFD06_10175"
     CDS             159..1136
                     /locus_tag="VFD06_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN03939.1"
                     /translation="MIDRRRFLLTSLAGVVAVPLAAGGQQAGKIYRIGTIWTTPTPSA
                     EDTLRQALRELGWIEGQNFVFERRYSEGRNDRLPVLAAELVRLRPDLISTVSDSAALA
                     AKAATTTIPVVFMAVGDPVGSGIVESLARPGRNLTGTGSSGSELVGKWLELFKEAVPS
                     LSRVGVFINSADPLNASARPIVETTGRHLGLALTYIEVRTPEDVDGAFAAVARENLRA
                     VVVLGQPLLFALRARLAKLALDQRVATMVPWRAAVEEGALISYLEALDWHLRRAANYI
                     DRILKGAKPADLPVERPTEFLLTINLKTAKALGLTIPPSLLARADQVIE"
     gene            1279..1704
                     /locus_tag="VFD06_10180"
     CDS             1279..1704
                     /locus_tag="VFD06_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03940.1"
                     /translation="MRKLERDLGRVVPYLFPHLEGRHRGERIQEFRKSGATARLKAGL
                     ASVETVKDGRRTVKKITAHRLQHDFRRTAVRNLVNVGVPEVVAMKITGHRTRSVFDRY
                     HIVSPEDLKAASRKLAAGTEGTVSGHSRQTAAITRIANR"
     gene            complement(1711..1788)
                     /locus_tag="VFD06_10185"
     tRNA            complement(1711..1788)
                     /locus_tag="VFD06_10185"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1751..1753),aa:Asp,seq:gtc)
BASE COUNT          346 a          689 c          658 g          334 t
ORIGIN      
        1 gccctgttcg tggacaagat cccgaagggt gctaagcccg gcgaccttcc cgttgagcag
       61 ccgacgaagt tcgagctggt catcaacctc aagacagcca aggccctcgg cctcacgatc
      121 ccgccgtcgc tgctggcgcg ggcggatcag gtgattgaat gatcgatcgg cgccgcttcc
      181 tgctgacctc gctggccggg gttgtcgccg tgccacttgc cgctggggga cagcaggcgg
      241 ggaagatcta ccgcatcggt acgatctgga ccacgccaac ccccagcgcg gaggacacgc
      301 tgcggcaggc ccttcgcgag ctcggctgga tcgaaggcca gaacttcgtc ttcgagcgac
      361 gctactccga ggggcggaac gatcggcttc ccgtgctcgc cgccgagttg gttcgcctcc
      421 gtcccgatct catcagcacg gtgagcgatt cagccgcact cgccgccaag gcggcgacga
      481 cgacgattcc cgtcgtcttc atggccgtgg gcgatcccgt ggggtcgggt atcgtcgaga
      541 gtcttgcccg accgggcagg aatctcacgg gaacgggtag ttcaggctcg gaactggttg
      601 gcaaatggct ggaactcttc aaggaggcgg ttccctcgct gtcgcgcgtc ggggtcttca
      661 tcaactcggc tgaccctctg aatgcctcag cccggccgat cgtcgagacg accgggcgac
      721 acctcggtct ggccctgacg tacatagagg tcaggacacc ggaggatgtc gacggcgcgt
      781 tcgcggcggt cgctcgcgag aacctccgcg ctgtcgtcgt gctcggccag ccgctgttgt
      841 tcgccctccg cgccaggctc gcgaagctgg cactcgacca gcgggtggcc accatggttc
      901 cctggcgggc ggcggttgag gagggcgccc tcatctccta tcttgaagcc ctcgactggc
      961 acttgcgacg cgctgcgaat tacatcgacc gcatcctcaa gggcgcgaag ccggcggatc
     1021 taccagtcga gcgaccgacc gagttccttc tcacgatcaa cctcaagacc gccaaggccc
     1081 tcggcctcac gatcccgccg tcgctgctgg cgcgggcgga tcaggtgatt gagtagccgc
     1141 ctgctctggg ccactcgttg tccgacgcgc gcggccgcct cctctatgcc gctctgggct
     1201 tcgtgaccct cggagccgca ccacgccatc ccgtcctcgc ccgaacttgc tgacctcatc
     1261 caggcgcagg ttgcccgcgt gcgcaagctg gagcgcgacc tggggcgcgt ggtcccctat
     1321 ctgttccctc acctcgaagg ccgtcaccgg ggagagcgga ttcaggagtt tcgcaagtcg
     1381 ggggcgacgg cccgcctcaa ggcgggactt gcctcagtag agacggtcaa ggacggccgc
     1441 cgcacggtga agaagattac cgcgcaccgt ctacaacacg acttccggcg caccgctgtg
     1501 cggaacctcg tcaacgtggg ggtgcccgag gtcgtggcca tgaagatcac cgggcatcgg
     1561 acccgctcgg tgttcgaccg ctaccacatc gtcagtcccg aagacctgaa ggcggcatcc
     1621 cggaagctcg ctgcgggcac ggagggcaca gtctcagggc acagtcggca gacggctgcg
     1681 atcacgagga tcgcaaaccg ttgattgaaa tggcgggctc gacgggactt gaacccgcga
     1741 cctctggatt gacagtccag tgtgctaacc aggctgcacc acgagcccgc accgccgacg
     1801 attgagcctc caaacgctaa cacgcgtcgc gcggagggtc aacgcgccga ccgccgcgcg
     1861 gcgcaggccc ggcagagatt acaacgaggg ggacagcgac gctgtccccc ctaccaacgc
     1921 ggcgaacgga tcaggggtca gagcgacagc tgacggccgt cgtctcgcgg gggcagctcc
     1981 acgcgggcga tgcgtagctt tccttcctgc gccagcatcg agagcac
//
LOCUS       DASRIQ010000418         4795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_703789, whole genome shotgun sequence.
ACCESSION   DASRIQ010000418 DASRIQ010000000
VERSION     DASRIQ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703789"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1173
                     /locus_tag="VFD06_10190"
     CDS             136..1173
                     /locus_tag="VFD06_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZN03941.1"
                     /translation="MERPVAPLGRADRVLVVRLGAVGDVIRALPALHRLRLTFPGAHL
                     AWVVEDLAAPLLERHPDLNEVIRLSRRDLRAAGRSPRRLARTVSDIGRRLRAGRYDVA
                     VDLQSSLKSGILTVLSGARRRVGFAPGHCREMSFLFTNEWAELSSPCLNRVDRNLEMA
                     AQLGAAEGPVSASLGETASEGAAAEGILASLSLSRGGPVLLCPGASRRQAFKRWPAAA
                     WGRLGELLAAAGTSPIIVWGPGEEGLAEEIERTSRGGARRAPSTSLPLLAALLRRSAL
                     FIGADTGPMHLAWAVGCPVVALFGPTDPRLNAPYGDGHEVLAAPEGELRRLAPETVSA
                     AALRMLARRPR"
     gene            1170..2270
                     /gene="waaF"
                     /locus_tag="VFD06_10195"
     CDS             1170..2270
                     /gene="waaF"
                     /locus_tag="VFD06_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HZN03942.1"
                     /translation="MNRPDERAAAAGAIDPERTRRILVRANNWIGDVVMISPAVRALR
                     RRFPRASIAIVAKRWVLDALSGNPAYDELIEYDHDGLHRGLAGRTRLARRIRGGGTVD
                     LAILFQKAFDAAFLARLAGARIRVGYATDARAFLLTHPLPPPPRDRHHADAFLDLARA
                     VGCEEIDPLPSFHLGEKERSAADAILERRGFGVAPLLIALHPGASKGPRAWHAERFAA
                     LAERLRRRHAARFVLIGSAGERETLRIVRAGLPDADWIGPDDAPGLLAAAALLARCHL
                     FVGNDSGPMHLAAALGVPTVGLFGPGRPANTAPRGRPGTVLTIGRDYPCAPCRQDFFR
                     ECPPAPSGKPFCLEEIGVDEVERAALRLLGRA"
     gene            complement(2299..3144)
                     /locus_tag="VFD06_10200"
     CDS             complement(2299..3144)
                     /locus_tag="VFD06_10200"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HZN03943.1"
                     /translation="MNGPDLPGYETLRSGPRLWRVRPGLASALAASPAAALLEGEPPE
                     PGPPGAGRSAVMTLVLAGRPAYAKRGVHGGLARRLLGGVFWRDGRGRRVIEAAERLSA
                     AGVPTPGVLAAGWRTIAGPLAAYVLVTEAIPGGTSLQAFLGEQPLARRRRRLARAAGR
                     SVRALHDTGFVHADLNLANLVAAGGGEPDAPGGEARVFVIDLDGGRFCGRVGHRARRR
                     NLMRLLRSYEKWIAPSSPLDPRDALAFLSAYCGEDRDMLGTLARQLGGIRFRWRRLLW
                     RRRAT"
     gene            complement(3141..3932)
                     /gene="tatC"
                     /locus_tag="VFD06_10205"
     CDS             complement(3141..3932)
                     /gene="tatC"
                     /locus_tag="VFD06_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZN03944.1"
                     /translation="MPPNPETSTSPADLPVMGFLEHLDELRRRLLYSLAAIALAFALA
                     LTRARDILEFLLRPVRPFLGDQKPIFLDLTEPFLLYMKVGFLASLFLSAPFVLYQVWA
                     FISPGLYPRERRMAVPFVAFATIFFLLGGAFGYYYGFPAAVRFLLSIAGEFEPNLRIS
                     SLFTFESRILLGMGLVFELPTVIYFLARLGLVTPRFLWRRFNYAILIIFIIAAVVTPT
                     PDIVTQCLFAAPMIALYLLGILVAHIFGRPRRVEESKGPGRSAAS"
     gene            complement(3895..4206)
                     /gene="tatA"
                     /locus_tag="VFD06_10210"
     CDS             complement(3895..4206)
                     /gene="tatA"
                     /locus_tag="VFD06_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZN03945.1"
                     /translation="MFGSIGGPELLVIFVVALLIFGPRKLPELGKTLGRGLAEFRRAA
                     NDLRDSLETEVARESPKPAAPSLPAGGSPPPGTLESGADASATPPTDDAAESRDQHEP
                     R"
     gene            4306..>4795
                     /locus_tag="VFD06_10215"
     CDS             4306..>4795
                     /locus_tag="VFD06_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN03946.1"
                     /translation="MSRRSILTGVVFVLSGGLAGGLLGGRSLALPERGGDPVGTFTQI
                     LSVVQSRAAGPVDSRNVIEGAIRGMVRTLDPHTNYLDAEDYRHMLEEQQGSFSGLGIV
                     ISKPGHDKPLTVISPLEGTPAWNAGIRAGDVISQIEGTDTLDMMIDEALRRLKGPKGT
                     QVT"
BASE COUNT          665 a         1757 c         1742 g          631 t
ORIGIN      
        1 aaggtcccct cgtggggagg gcgcacttat agcacccgcc cgaaaaaact gtcaaaccgt
       61 ggccgcgccg cggcgcacca tgacctgcgc gccggggctc accggctccc ggcagcctgt
      121 taacatcccg ccgccatgga gcgccccgtc gcaccgctcg ggcgcgccga ccgggtcctc
      181 gtcgtccggc tcggcgcggt gggggacgtg atccgcgccc tccccgccct gcaccgcctc
      241 cgactgacgt ttcccggggc gcacctggcc tgggtggtgg aggacctggc cgcgccgctt
      301 ctcgagcggc accccgacct gaacgaggtg atccggctgt cgcggcgcga cctccgtgcc
      361 gccgggcgca gtccgcgccg gctcgcccgc accgtctccg acatcggacg gcgcctccgg
      421 gccgggcgct acgacgtggc ggtcgatctg cagtcgagcc tcaagagcgg gatcctcacc
      481 gtcctttcgg gagcgcggcg gcgcgtcggc ttcgcgccgg ggcactgccg cgagatgagc
      541 ttcctgttca ccaacgaatg ggcggagctt tcgtccccct gtctcaaccg ggtcgatcgc
      601 aatctcgaga tggcggcgca gctcggcgca gccgagggcc ccgtgagcgc gtcgctcggc
      661 gagaccgcct cggagggggc cgcggccgag gggatcctcg cttcgctcag tctctcgcgc
      721 ggcggtcccg tcctgctgtg ccccggcgcc agccggaggc aggcgttcaa gcgctggcct
      781 gcggccgcct ggggccggct cggggagctt ctcgccgccg ccgggacgag cccgatcatc
      841 gtctggggtc cgggcgagga ggggctggcg gaggagatcg aacggaccag ccggggcggc
      901 gcacgccgcg ccccctcgac ctcgctcccg ctcctcgcgg cgcttctccg gcgctccgcc
      961 ctgttcatcg gcgccgacac cgggccaatg cacctcgcct gggcggtcgg gtgcccggtg
     1021 gtcgctctgt tcgggccgac cgatccgcgc ctcaatgccc cctacggcga cggtcacgag
     1081 gtgctcgcgg cgccggaggg ggagctgcgg cggcttgcgc cggagaccgt ctcggcggcg
     1141 gccctgcgaa tgctcgcgcg gaggccgagg tgaaccggcc ggacgaacgg gccgcggcgg
     1201 cgggcgccat cgacccggag cgcacgcgcc gcatcctcgt ccgcgccaac aactggatcg
     1261 gggacgtcgt gatgatctct ccggcggtgc gggccttgag gcggcggttc ccgcgcgctt
     1321 cgatcgcgat cgtggcgaag cgctgggtgc tcgacgccct gtcggggaac cccgcctacg
     1381 acgagctgat cgaatacgac cacgatggcc tccatcgggg cctcgccgga cggacgcgcc
     1441 tggcacgtcg catccgcggc ggcggaacgg tcgatctcgc gatcctgttc cagaaggcgt
     1501 tcgacgcggc cttcctcgcg cggctcgccg gcgcccgcat ccgcgtcggc tacgcgaccg
     1561 acgcgcgcgc cttcctcctg acgcacccgc tcccgccgcc gccgcgcgac cggcaccacg
     1621 ccgacgcgtt cctcgatctg gcgcgggcgg tgggctgcga ggagatcgac ccgctgccgt
     1681 cgttccatct cggggaaaag gagcgctcgg cggccgacgc gatcctcgag cgccgcgggt
     1741 tcggggtcgc gccgcttctc atcgcgctcc acccgggggc ctcgaaggga ccgcgcgcct
     1801 ggcacgccga gcggttcgcg gcgctcgcgg agcggctgcg ccggcggcac gcggcccgct
     1861 tcgtcctcat cggatcggcg ggcgagcgcg aaacgcttcg gatcgtccgc gcggggctcc
     1921 cggacgccga ttggatcgga ccggacgacg cccccggact cctggccgcc gcggcgcttc
     1981 tcgcgcgctg tcatctcttc gtcgggaacg acagcggccc gatgcacctg gcggcggcgc
     2041 tgggagtgcc gaccgtcggg ctcttcggtc cggggcgccc cgccaatacc gctccccgcg
     2101 gccgccccgg caccgtgctg acgatcggac gcgactaccc gtgcgcgccg tgccgccagg
     2161 acttcttccg cgagtgtccg ccggccccgt ccggaaagcc gttctgtctc gaggagatcg
     2221 gcgtggacga ggtcgagcgg gcggcgctgc gtctcctggg ccgggcctga tcgggcctcg
     2281 cagtccgcgg cggcgggatc aggttgcgcg gcgccgccag aggaggcggc gccaccggaa
     2341 ccggatgccg ccgagctggc gcgcgagcgt cccgagcatg tcccggtctt cgccgcagta
     2401 ggcgctcaag aaggcgagcg cgtcgcgcgg atcgagcggg ctcgaagggg cgatccactt
     2461 ctcgtaggaa cgcagcaggc gcatgaggtt gcgccgcctc gcgcggtggc cgacccgccc
     2521 gcagaagcgt ccgccatcga gatcgatgac gaagacccgc gcctcgcccc cgggcgcgtc
     2581 cggctcgccg ccgccggccg ccaccaggtt cgccagattg agatcggcgt gcacgaaccc
     2641 ggtgtcgtgc agcgccctca cgctccggcc ggcggcgcgc gccagccggc gccggcgccg
     2701 cgccagcggc tgctcgccga gaaacgcctg caggctcgtc cctcccggga tcgcctcggt
     2761 caccagcacg taggcggcga gcggcccggc gatcgtcctc catccggcgg cgaggacgcc
     2821 gggcgtcggc acgccggccg cgctcaggcg ctcggccgcc tcgatcaccc gtcggccgcg
     2881 tccgtcccgc cagaagactc cgccgaggag gcggcgggcg agcccgccgt gcaccccccg
     2941 cttcgcgtag gccggtcgcc cggcgagcac cagggtcatc accgccgatc gtccggcgcc
     3001 cggcggcccc ggttcgggtg gctccccttc caggagcgcc gcagccggcg aggcggcgag
     3061 ggccgacgcg aggccgggcc gcacccgcca cagccgcggg ccggaccgaa gcgtctcgta
     3121 tcccggcagg tcggggccgt tcacgacgcg gcggagcgcc cgggcccctt cgactcctcc
     3181 acgcggcggg gccgcccgaa gatgtgggcc acgaggatgc cgaggagata gagggcgatc
     3241 atcggcgccg cgaacaggca ctgcgtgacg atgtcggggg tcggcgtgac caccgcggcg
     3301 atgatgaaga tgatcaggat ggcgtagttg aaccggcgcc acaggaaccg cggcgtcacc
     3361 agccccaggc gcgccaggaa atagatgacc gtcggcagct cgaacaccag ccccatgccg
     3421 agcaggatgc ggctctcgaa cgtgaacagg ctgctgatcc tgagattcgg ctcgaactcc
     3481 cccgcgatcg agagcaggaa ccggaccgcc gccgggaagc cgtagtagta gccgaacgcg
     3541 ccgccgagca ggaagaagat ggtcgcgaag gcgacgaacg gcaccgccat gcgcctctct
     3601 cgtggataga gcccggggga gatgaacgcc cacacctggt acaggacgaa gggggccgag
     3661 aggaacagcg aggccaggaa ccccaccttc atgtagagga ggaacggctc ggtcagatcg
     3721 aggaagatcg gtttctgatc gccgaggaag ggccggaccg ggcgcagcag gaactcgagg
     3781 atgtcgcggg cgcgcgtcag cgccagcgcg aaggccagcg cgatcgccgc gaggctgtag
     3841 agcagacgcc ggcgcagctc gtccaggtgc tccaggaacc ccatgaccgg gaggtcagcg
     3901 gggctcgtgc tggtctcggg attcggcggc atcgtcggtc gggggggtgg cgctcgcgtc
     3961 ggcgccggac tcgagcgtgc ccggaggcgg cgacccgccg gccgggagcg agggcgccgc
     4021 cggcttcggg ctctcgcggg cgacctcggt ctcgagcgag tcgcgcaggt cgttggcggc
     4081 ccggcggaac tccgcgagcc ctcgacccag agtcttcccg agctcgggca gcttgcgcgg
     4141 cccgaagatc agcagcgcca cgacgaagat gacgaggagc tccggtcctc cgatcgaacc
     4201 gaacatgccg cgcgcatctt agcattccgc ccaaaagccc gaatttcctt ccttgtgcgg
     4261 agccgatcct cgccctataa ttccgcgcag cgttacggag gctgagtgag tcgacgttcc
     4321 atcctgaccg gggtcgtttt tgtgctatcc ggcggtctcg ccggcggcct cctcggtggg
     4381 cggtccctgg ccctcccgga gcgggggggg gacccggtcg gcaccttcac ccagatcctc
     4441 tccgtggtgc agtcgcgggc cgccggcccg gtcgattccc gaaacgtgat cgaaggcgcg
     4501 atccgcggca tggtccgcac cctcgatccg cacacgaact acctcgacgc cgaggactac
     4561 cggcacatgc tggaggagca gcagggctcc ttctccgggc tcggcatcgt catctcgaag
     4621 ccgggacacg acaagcccct cacggtgatc tcgccgctcg aggggacccc ggcgtggaac
     4681 gcggggatcc gggcgggcga cgtgatctcg cagatcgagg gaaccgacac cctcgacatg
     4741 atgatcgacg aggcgctccg ccgcctgaag ggaccgaagg ggacgcaggt cacga
//
LOCUS       DASRIQ010000419         7325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_175253, whole genome shotgun sequence.
ACCESSION   DASRIQ010000419 DASRIQ010000000
VERSION     DASRIQ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175253"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..989)
                     /locus_tag="VFD06_10220"
     CDS             complement(99..989)
                     /locus_tag="VFD06_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN03947.1"
                     /translation="MTPELIIEGVSKTYPNGVKALRNVSLRIPAGMFGLLGPNGAGKS
                     TLMRTLATLQEPDTGSIRLGTIDVLREKETVRRMLGYLPQEFGVYPKVSAQELLDHFA
                     VLKGILNARQRKETVDALLERTNLTDVRKRRLGTFSGGMKQRFGIAQALLGDPKLIIV
                     DEPTAGLDPVERMRFNNLLSRISEQVVVILSTHIVDDVSDLCRNMAIIDQGQVLLTGD
                     PLKTIDQLRGRVWKKIVARDDLQAYERDCHVISTRLVAGRTQIHVLSGNRPDPSFEEV
                     PPGLEDVYFSTLGAAKEQAA"
     gene            complement(1142..1357)
                     /locus_tag="VFD06_10225"
     CDS             complement(1142..1357)
                     /locus_tag="VFD06_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03948.1"
                     /translation="MLFRILGVAALVAGIVILVYGGFSVPNDRDAKLGPIEVKVQQSE
                     KVPIPTWAGVASIAFGGLLLLWSGKRK"
     gene            complement(1359..3041)
                     /locus_tag="VFD06_10230"
     CDS             complement(1359..3041)
                     /locus_tag="VFD06_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03949.1"
                     /translation="MPEPAPAATPPTARRDTALLLVVVCAAAALLWRGGAMARAHSES
                     YDDEYHLVHGAAYFRGGLNELAQPDPPLGQALTALPMVLSNSNPRWTGALYDHSRPPE
                     TVLFRVALWRSLLFLPAVAVAFLWCRRLYGASAGWLAAALFVAEPNLAAHIPLAALDA
                     LGVVGALVGSFLLWRAFERPSPGRGAAAAAGCAAALLLKGTTTLLPAVAAAYALLFWV
                     VRPWRATRRTNGAAAPDSRDWRRALPRRLAAFAGFAALAALVLWALLLFDVSRPDQPP
                     EWQQAPTTLSRLLDRPLPAGLFVGSLVRGAWHADQGHLAYLLGERSRSGWWYYFPVVM
                     AYKMPLGIALVLLCALLSLRRAPPRFEEWGLVVPAVLWTALFLTTRINIGVRHFLPAY
                     AFLLLLGARALAGDPPRWRAAAAWGGATLAALHGFLWHPDYIAYQNLPRARAYMDLSD
                     SNVDWGQGLKQARHWIDRHPQPPGQPIFLLYFGDPNSPRRVLHYLGNRVTLIPPHAPL
                     PASGLLIASPVWVAGPFDTGDRYVRLRGARPIDVIGHTLLVYDLAALEGGDT"
     gene            3094..3492
                     /locus_tag="VFD06_10235"
     CDS             3094..3492
                     /locus_tag="VFD06_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN03950.1"
                     /translation="MSARQRVSTGAPWEPIVGYSRAVRVGNTVHVSGTTALDAEGRLV
                     GRGDARAQTVQTLRNIEAALAKAGARLDDVVRTRMYVTDIARWEEIGRAHGEVFREIR
                     PASTMVEVKRLIDPDMLVEIEAEAIVDDRR"
     gene            3535..3951
                     /locus_tag="VFD06_10240"
     CDS             3535..3951
                     /locus_tag="VFD06_10240"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZN03951.1"
                     /translation="MGTEIDIEYEGDLHCRLHHGPSGAEIVTDAPLDNQGKGEAFAPT
                     DLLAASLGSCILTIMGIYARRKKIEMRGSHARVSKEMVADPLRRVGRIVVDVHLPVSI
                     ESPLRPALEIAGHSCPVERSLHPDIKVELNFRYDQP"
     gene            complement(3959..6241)
                     /locus_tag="VFD06_10245"
     CDS             complement(3959..6241)
                     /locus_tag="VFD06_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HZN03952.1"
                     /translation="MSAVVRRARVPGTILAALLVCIAWWPAVATAAATKDQPALQRPP
                     TLEERRGVRKSHARAGDASIESTPSPLAAAPLTMDVERYTISILVTLPGPGLGRVDGT
                     VRIQARPVGGPATTLTFGLDSVMSLSSVRKTGGALLATSRLGNTVQVTLDRSYAVGEL
                     IDLTVTYGGTPPSVNVSGLAFAFRTHGAGTPGQGPIVSSLSQPDFAPAWWPCLDRPDD
                     KAIVDMDLRVPAALTGVSNGMLVANILNGDGTRTFQWRSVYPISTYLVSVAISNYVTW
                     TDFYTPVTGGPVMPVQHWVYPEHETAARTDLNVTVPMLTFFSNTYGEYPFVAEKYGHA
                     IFPFGGGMEHQTVTSYGGGLIRGDHLYDWIVAHELAHQWFGDAIGPAEWPEIWLNEGF
                     ATYGEVLWQEHLGGAAAMRNYVQSLDSRPFGCPVYDPLPECDDLFDHTVYDKGAWVLH
                     MLRHVVGDAAFFQGLRNYYTAFNGSSATTPGFLATMEVASGRDLDAFFDRWVYQMGEP
                     VYAFGWTAASTPAGWVTHVHIDQQQGGILFEMPVDIRVSWNEGSQTFVVNNTSFGQDT
                     ALPPVPGQPTQVVFDPDLWILKTLNLFTPPDGDGDGVPDTADNCSGASNPAQEDLDGD
                     GLGNACDPDLDGDGRANGADCAPADPTAQDPPGTEVSGVTMNGALAATIAWTPLGGAP
                     PTWSYDVLRGSLLALRAAGNLSGLACFAGSQPPSSLPDLTAPPPGDGLYYFVRARNPC
                     GTGPVGNSSNGTPRPSPACP"
     gene            6425..>7325
                     /locus_tag="VFD06_10250"
     CDS             6425..>7325
                     /locus_tag="VFD06_10250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZN03953.1"
                     /translation="MSPPTVTATAPPIGVAELRSEAPEVRPPILPAPKAITMPPTRLL
                     IAWGLGCTGVGAFVAAGIAFTRSGAGFGPILLISILFAEVVGFTALLSARMVFPLFER
                     LPAAANLGLQILTLLSGTIFGSILVIGMQPYFALARLRTVAMIVVINAVLGVIVGIAL
                     RTYDSMREQIEAQYQALRSKEAMEREIEIAREVQRELLPRVCPVVPGLRLAGACRPAI
                     GVGGDYYDYLQFSDHRLGIVIADVAGKGIPAALLMAGLQASVRSLAGPTVEPGEVNRL
                     LNGMLYRSTTTARYATLFFALYDA"
BASE COUNT         1136 a         2461 c         2710 g         1018 t
ORIGIN      
        1 cgaagaagac cagcacgaag aacaggatcg aggtgatcca gatcagcggc tgccggaact
       61 ggtagcggaa ctcgaacgcg aagatgcggg ccagcatctc aggccgcctg ttccttggcc
      121 gcgccgaggg tcgagaagta aacgtcttcg agccccggcg gcacctcctc gaaggaagga
      181 tcggggcggt tcccggagag cacgtggatc tgcgtccggc ccgcgacgag ccgcgtggag
      241 atgacgtggc agtcgcgctc gtaggcctgc agatcgtcgc gcgccacgat cttcttccag
      301 acccggccgc gcagctggtc gatcgtcttc aggggatcgc cggtcaggag gacctgtccc
      361 tggtcgatga tcgccatgtt ccggcacagg tcgctgacgt cgtccacgat gtgggtggag
      421 aggatcacga ccacctgctc gctgatccgg ctcaggaggt tgttgaagcg catccgctcg
      481 accggatcga ggccggcggt cggctcgtcc acgatgatga gcttcggatc gccgaggagc
      541 gcctgggcga tgccgaagcg ctgcttcatc ccaccggaga aggtccccag gcgccgcttg
      601 cggacgtcgg tgaggttggt gcgctcgagc agggcgtcga ccgtctcctt gcgctggcgc
      661 gcgttgagga tccccttgag cacggcgaag tgatcgagca gctcctgggc gctcactttg
      721 ggatacacgc cgaactcctg cggcagatac ccgagcatcc ggcgcacggt ctccttctcc
      781 cggaggacgt cgatcgtgcc cagccggatc gagccggtgt ccggctcctg cagcgtcgcc
      841 agggtgcgca tgagcgtgga cttgccggcg ccgttcgggc cgagaaggcc gaacatcccc
      901 gccgggatgc gcagcgagac gttgcgcagg gccttgacgc cgttgggata ggtcttggag
      961 acgccttcaa tgatgagttc cggggtcatc gcccgatctc cttcgagagc ttggccgtgt
     1021 ggggtgctga cggcccgatc ctacgcccgg agtggccggg cggttcccgc ggggcgggga
     1081 aaaggagggg gcggagggcg gtcccgtccg ggtccggccg atcggccggc gcgcgacgac
     1141 gttatttcct cttgccggac cagaggagca ggaggccgcc gaaggcgatg ctggcgacgc
     1201 cggcccaggt cggaatcggc accttctcgc tctgctgcac cttgacctcg atcgggccga
     1261 gcttcgcgtc gcggtcgttg ggcacgctga agccgccgta gaccaggatg acgatccccg
     1321 cgacgagcgc ggccacgccc aggattctga agagcatttt aggtgtcccc tccttcgaga
     1381 gcggcgagat cgtaaaccag aagcgtgtgg ccgatgacgt cgatcgggcg ggcgccgcgc
     1441 aggcgcacgt agcgatcgcc ggtgtcgaac gggcccgcca cccagaccgg gctcgcgatc
     1501 agcaggccgc tggcggggag cggcgcgtgc ggcgggatca gggtcacgcg gttgccgagg
     1561 tagtgcagga cccgtcgggg gctgttcggg tcgccgaagt agaggaggaa gatcggctgg
     1621 cctgggggct gcgggtggcg gtcgatccag tggcgcgcct gcttgagccc ctgcccccag
     1681 tccacgttgc tgtcgctcag gtccatgtag gcgcgcgccc gcggcaggtt ctggtaggcg
     1741 atgtagtcgg ggtgccagag gaacccgtgc agcgccgcga gggtcgcccc accccacgcg
     1801 gcggccgcgc gccagcgggg cggatcgccc gcgagggcac gggcgccgag caggagcagg
     1861 aacgcgtacg ccggcaggaa atgccgcacg ccgatgttga tgcgcgtcgt caggaagagg
     1921 gcggtccaga gcacggcggg gacgacgagc ccccactcct cgaagcgggg cggggcgcgg
     1981 cgcagcgaca ggagggcgca cagaagcacg agcgcgatgc cgagcggcat cttgtacgcc
     2041 atgacgaccg ggaagtagta ccaccacccg ctccggctgc gctctccgag caggtaggcg
     2101 aggtgcccct ggtcggcgtg ccaggcgccg cgcacgaggg agccgacgaa gagtccggcc
     2161 ggcagcgggc gatcgagaag acgactgagg gtggtcggtg cctgctgcca ctcgggcggc
     2221 tggtcggggc ggctgacgtc gaacaggagc agcgcccaga ggacgagcgc cgccaatgcc
     2281 gcgaagccgg cgaaggcggc cagccgccgc ggcagcgccc gccgccagtc ccggctgtcc
     2341 ggcgccgcag cgccgttcgt ccggcgcgtc gcgcgccacg gacggacgac ccagaagagc
     2401 agcgcgtacg ccgcggccac ggccggcagg agggtcgtcg tccccttcag gagcagggcc
     2461 gccgcgcacc cggccgccgc cgcggcgccg cgtccgggag acggccgctc gaaggcgcgc
     2521 cagagcagga atgacccgac gagcgccccg acgacgccga gggcgtcgag tgccgcgagc
     2581 gggatgtgcg ccgcgagatt cggctccgcg acgaaaagcg ccgccgccag ccacccggcc
     2641 gaggcgccgt agaggcggcg gcaccagaga aacgccacgg cgaccgccgg caggaacagg
     2701 agcgaccgcc acagcgcgac gcggaacagg accgtctccg gcgggcggga atgatcgtag
     2761 agggcgccgg tccagcgcgg gttcgagttc gacagcacca tcggcagggc ggtcagcgcc
     2821 tggccgagcg gcggatccgg ctgcgcgagc tcgttcaggc cgccgcggaa gtaggcggcg
     2881 ccgtgcacca ggtggtactc gtcgtcgtac gactcactgt gggcgcgcgc catcgccccg
     2941 ccgcgccaca ggagcgccgc ggcggcgcac acgacgacga ggaggagggc cgtgtcgcgc
     3001 cgcgcggtcg gcggcgtcgc ggcgggcgcg ggttccggca tggaggggca tgttacactc
     3061 cgggccaaat ccgaccggag gacgttcacg acgatgagcg cacggcagcg agtctccacc
     3121 ggcgcgccgt gggagccgat cgtcggctac tcgcgcgccg tgcgcgtcgg gaacacggtg
     3181 cacgtctccg ggacgaccgc cctcgacgcc gagggccggc tcgtcggacg cggcgacgcc
     3241 cgcgcccaga cggtgcagac gctcaggaac atcgaggcgg cgctggcgaa agcgggggcc
     3301 aggctcgacg acgtggtgcg gacccgcatg tacgtcaccg acatcgcgcg ctgggaggag
     3361 atcggccggg cgcacggcga ggtgttccgc gagatccggc cggcctccac catggtggag
     3421 gtcaagcggc tcatcgatcc cgacatgctg gtggagatcg aggcggaggc gatcgtcgac
     3481 gatcgccgct gagcgccggg cgccggaccg gatgcggatc gaaaaggagc gaggatgggg
     3541 accgagatcg acatcgagta cgagggggac ctgcactgcc ggctgcacca cggcccgtcg
     3601 ggggcggaga tcgtcaccga cgccccgctc gacaatcagg ggaaggggga ggcgttcgcg
     3661 ccgaccgatc tcctggccgc ctcgctcggc tcctgcatcc tgacgatcat ggggatctac
     3721 gcgcgccgca agaaaatcga gatgcgcggc agccacgcga gggtgtcgaa ggagatggtc
     3781 gccgacccgc tccggcgggt cgggcggatc gtcgtcgacg tccatctgcc agtgtcgatc
     3841 gagtcgccgc tgcgaccggc gctcgagatc gccgggcatt cctgtccggt cgagcgcagc
     3901 ctccaccccg acatcaaggt cgaactgaac ttccgctacg accagccctg accgcgggct
     3961 aaggacaggc cggcgaaggt ctcggagtgc cgttcgagga gttgccgacc ggaccggtgc
     4021 cgcaggggtt ccgcgcccgg acgaagtagt agaggccgtc ccccggcggc ggggccgtca
     4081 gatccggaag ggatgacggc ggctgcgagc ctgcgaagca ggcgagcccc gagagattcc
     4141 cggctgcgcg cagcgccaga agcgatccgc gcaggacatc gtagctccag gtcggaggag
     4201 cgccgccgag cggcgtccag gcgatcgtcg cggccagggc accgttcatc gtgacccccg
     4261 acacctccgt cccgggggga tcctgcgccg tcgggtcggc cggcgcgcag tcggcgccgt
     4321 tggcgcgtcc gtccccgtcg agatccgggt cgcatgcgtt cccgagaccg tcgccgtcga
     4381 gatcctcctg cgccgggttc gaggcgccgc tgcagttgtc ggcagtgtcc ggcaccccgt
     4441 cgccgtcccc gtcgggcggc gtgaagaggt tgagggtctt gagaatccag agatcggggt
     4501 cgaacaccac ctgggtgggc tgccccggga ccggcggcag cgccgtatcc tgcccgaagg
     4561 aggtgttgtt caccacgaag gtctgcgacc cctcgttcca ggagacgcgg atgtcgaccg
     4621 gcatctcgaa aaggatgccc ccctgctgct ggtcgatgtg cacgtgcgtc acccaccccg
     4681 ccggggtcga cgcggcggtc cagccgaagg cgtacaccgg ctcccccatc tggtagaccc
     4741 agcgatcgaa gaacgcgtcg agatcgcggc cggaggcgac ctccatcgtg gcgaggaagc
     4801 ccggcgtcgt ggcgctcgat ccattgaacg ccgtgtagta gttgcgcagc ccctggaaga
     4861 acgccgcgtc gccgacgacg tgccgcagca tgtgcagcac ccaggccccc ttgtcgtaca
     4921 cggtgtggtc gaacaggtcg tcgcattccg gcagagggtc gtaaaccggg cagccgaagg
     4981 ggcgcgagtc gagcgactgc acgtagttgc gcatcgcggc ggcgccgccg aggtgctcct
     5041 gccacagcac ctcgccgtag gtcgcgaatc cttcgttcag ccagatctcc ggccactcgg
     5101 ccggtccgat cgcgtcgccg aaccactggt gcgccagctc gtgcgccacg atccagtcgt
     5161 agaggtgatc gccgcggatg agcccgccgc cgtaggaggt gaccgtctgg tgctccatcc
     5221 ctccgccgaa cgggaagatg gcgtggccgt acttctccgc gacgaacggg tactcgccgt
     5281 aggtgttcga gaagaaggtc agcatcggca cggtgacgtt gaggtcggtg cgcgccgccg
     5341 tctcgtgctc cgggtagacc cagtgctgca ccggcatcac cggtccgccg gtcaccgggg
     5401 tgtagaagtc ggtccaggtg acgtagttgg agatcgccac cgacacgagg taggtgctga
     5461 tcgggtagac ggagcgccac tggaaggtgc gcgtgccgtc gccgttcagg atgttggcga
     5521 cgagcatccc gttgctcacc ccggtgagcg ccgcggggac gcgcagatcc atgtcgacga
     5581 tcgccttgtc gtccggccga tcgaggcacg gccaccaggc cggcgcgaag tccggctggg
     5641 acagggacga gacgatcggc ccctgccccg gcgtgccggc tccgtgggtg cggaacgcaa
     5701 aggcgagccc gctgacgttc accgacggcg gggtgccgcc gtacgtgacc gtgaggtcga
     5761 tcagctcgcc gacggcgtag gagcgatcga gcgtgacctg cacggtattg ccgaggcgcg
     5821 acgtggcgag gagcgcgccg ccggtcttcc ggaccgagct gagcgacatc acgctgtcca
     5881 ggccgaaggt gagggtcgtg gccgggccgc cgaccggacg cgcctggatg cggaccgtgc
     5941 cgtccacccg acccaatccg ggccccggca aggtcaccag gatcgagatc gtatagcgct
     6001 cgacatccat ggtgagtggg gcggcggcga gcggcgatgg cgtcgactcg atcgaggcgt
     6061 cgccggcccg cgcatggctc ttgcggaccc cgcggcgctc ctccagcgta ggtggtctct
     6121 ggagggccgg ctggtccttg gtcgcggcgg ccgtcgcgac ggcgggccac cacgcgatgc
     6181 acaccagcag tgcggccagg atcgtccccg ggacccgggc gcgccggacg acggcgctca
     6241 tcaggcggaa aaaacggggg aggggtggat ccggcatggt tggcggctgg aagtgtacgc
     6301 aaagaaggga tgcggcgcca actgagaatt gccgccgcac gctcccccgc ggcccgcgcc
     6361 gtgagccggt tccgtcacct gcgcaacccg gtcaccacgc tatacttccc cccggagccc
     6421 ccaattgagc cctccgacgg tgaccgccac cgcccccccg atcggggtcg cggagctcag
     6481 gagcgaggct cccgaggtcc ggccccccat cctcccggct ccgaaggcca tcacgatgcc
     6541 gccgacgcgg ctcctgatcg cgtgggggct cgggtgcacc ggcgtcgggg cgttcgtcgc
     6601 ggccggcatc gccttcacga ggagcggcgc cggcttcggc cccatcctgc tcatcagcat
     6661 cctgttcgcc gaggtggtcg ggttcacggc gctcctctcg gcccggatgg tcttcccgct
     6721 gttcgagcgg ctgccggcgg cggcgaacct gggtctgcag atcctcaccc tcctgtccgg
     6781 gacgatcttc gggtcgatcc tggtgatcgg catgcagccg tacttcgccc tggcgcgcct
     6841 gcgcacggtg gcgatgatcg tcgtcatcaa cgcggtgctc ggcgtcatcg tcggcatcgc
     6901 gctgcgcacc tacgactcga tgcgggagca gatcgaggcg cagtaccagg cgctccggtc
     6961 gaaggaggcg atggagcggg agatcgagat cgcccgcgag gtgcagcggg agctgctgcc
     7021 gcgcgtctgt ccggtggtgc cggggctgcg cctcgccggg gcatgccggc cggccatcgg
     7081 agtcggcggc gactattacg attacctgca gttctccgat caccggctgg ggatcgtcat
     7141 cgccgacgtc gccggcaagg ggatcccggc ggctctgctg atggccgggc tgcaggcctc
     7201 ggtgcgcagc ctcgccgggc cgacggtcga gccgggggag gtgaaccgcc tcctcaacgg
     7261 catgctgtac cgctccacca ccacggcccg ctacgccacc ctgttcttcg ccctctacga
     7321 cgccg
//
LOCUS       DASRIQ010000420         4752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3280832, whole genome shotgun sequence.
ACCESSION   DASRIQ010000420 DASRIQ010000000
VERSION     DASRIQ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3280832"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFD06_10255"
     CDS             <1..239
                     /locus_tag="VFD06_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HZN03954.1"
                     /translation="RASRDLGLRPVVAVNPRNAGAADVAFTEGLVEMALDGIGMRGRD
                     GHTTKETADMGSLRTQARRAAVLLARLASEPRKP"
     gene            252..3485
                     /locus_tag="VFD06_10260"
     CDS             252..3485
                     /locus_tag="VFD06_10260"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN03955.1"
                     /translation="MSSISEYRRRARAAARAADYSQSGDFYRLAGDWQKATEMYLRGG
                     HFDLAASLAEEMGDLASASLHYLKAGDLRAAADIELRLENKDKAAWLYSRAGLCTRAA
                     ELFEALDQPEQAAENLERSGAKERAALLYVRAGQPAHAVRLFEALIAAAAQSGPESML
                     TEGERAELSRWHRLCAELMVKVGRPRDAAPHFEAALLMEQAAQAWRKAGEPEKAAEIF
                     LRLQRPDDAWQALHEAGKDLSGVSPAVQAAILSRQGKHREAAEVLERAGSLYPASEEW
                     KKAGDLLRAARLMEKEGETDQAADLYARAGHPAEAAALMEKTRDFGAAATLFKSAGQH
                     EDAARMLLRAGDPLGAARLSYERGDREGCIRALQQVKRDSAEFRDASMLLGKIFSEQG
                     LFTLAADKFQAALDGEEVSDETVGIYYALGRAHEGNGRAREALRVYESILAFAYGHED
                     VLGRVRALRSKPLTTGPNPAPAPTEAVAGPRVATATSRTRVAPTPGVTGKSPVLPPGG
                     ARSAPAAGAPAAAPAERRRPHHEPAATVAPAVENGAARYVIGKPIGRGLSGEVFRGVD
                     SVLGRPVALRRIHEGPAEAGKAARLLQAAAEAASLSHPHIVPVYDTGQDARGPFIVAA
                     LAEGPSLRSLLQAKVRFELHRVVDIGRQIASALEHAHGRGVLHRNLRPENIHIAPDDR
                     VAVSDFGLSVRLSDLTPEELSSGRLIQYTPPEALLRGRVDVGSDLYSLGVILYELAVG
                     HPPFRGHDIGHQHVNDPVPLPGPGERLLPEFLKNVVLRCLEKDRARRYPDAKTLLEDL
                     VLREIVPGMVVSGRYQVLAEIGRGGMGAIFRARDAELDETVAMKVLAADIDAETAARF
                     VQEIKIARSIVHPNVVRVHTFEKWRDFRLLMMEYIDGVPLPRWMERVPRPSRADRLHV
                     AMQAAAGLEAAHRAGIVHRDIKPENILVTGAGQAKVLDFGIARPESAGHTLTEKGLVL
                     GTPRYMSPEQVQGKPLDRRSDIYSLGAVLFFLFTGEEPFVGSDVRETLLTHLRPPVKT
                     PLNIDPSLPVPLSQAILKALEVDPDGRFPTADAFAAALARAVEPRAA"
     gene            complement(3489..4619)
                     /locus_tag="VFD06_10265"
     CDS             complement(3489..4619)
                     /locus_tag="VFD06_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HZN03956.1"
                     /translation="MNPSTVQTPLRAIAAAAIALLSAASATGLPAAPNGTAAPQSAGF
                     YEQTNLVSDEPGVALIQDPDLVNPWGISLSPAGGAFWVANNETGTATLYTGDVEGSTF
                     SKAALTVAIPGGVPTGTAFNPTADFVVSAGADSGPALFLFVSLGGQVTGWNPAVPPPP
                     PSTTAQVAAVAEATYTGMTIGQTSTGANVIYAADFENGKIDVFDADFDLTELDGSFTD
                     PDLGRSLSPFNIQNIGGRLYIAYARHSARDPEEETSHGPGAVNVYDTDGHLLQQLIGH
                     GGRLVAPWGMALAPADFGPFSNDLLVGDFATGRILAFDPETGEFLGFMRGEDARPIAI
                     EGLWGILFGNGVTAGDADTLYFAAGPDDETHGLFGSLRFAGD"
BASE COUNT          712 a         1643 c         1736 g          661 t
ORIGIN      
        1 atcgggcgag ccgggacctc gggcttcggc cggtggtcgc ggtgaacccg cgcaacgccg
       61 gggcggccga cgtcgcgttc accgagggcc tcgtcgagat ggcgctcgac ggcatcggga
      121 tgcgtggccg ggacgggcac acgacgaagg agaccgccga catgggctcc ctgcggaccc
      181 aggcccgccg cgccgccgtc ctcctggccc ggctggcgtc cgaacctcgc aaaccctaga
      241 tttcaccggt catgtcctcg atctccgagt accggaggcg ggcccgcgcc gccgccaggg
      301 cggccgacta ttcccagtcg ggggacttct accgtcttgc cggggactgg cagaaggcga
      361 ccgagatgta cctgcgcggg gggcacttcg acctcgccgc cagtctcgcc gaggagatgg
      421 gggatctcgc ctccgcctcg ctccactacc tcaaggcggg ggacctccgg gcggcggccg
      481 acatcgagct gcgcctcgag aacaaggaca aagcggcctg gctctacagc cgggccggtc
      541 tgtgcacccg cgccgccgag ctgttcgaag cgctcgacca gccggagcag gcggccgaga
      601 acctggagcg atcgggggcg aaggagcggg cggccctcct ttatgtacgg gcggggcagc
      661 cggcgcatgc cgtgcgcctc ttcgaggcgc tcatcgcggc cgcggcgcag tcgggtcccg
      721 aatcgatgct gaccgagggg gagcgggcgg agctgtcccg ctggcaccgc ctgtgcgccg
      781 agctcatggt gaaggtcggc cggcctcggg atgcggcgcc gcacttcgag gcggccctcc
      841 tgatggagca ggcggcgcag gcctggcgca aggcggggga gcccgagaag gcggcggaga
      901 tcttcctccg gctgcagcgc cccgacgatg cgtggcaggc gctgcacgag gcggggaagg
      961 acctttccgg cgtctcgccg gcggtgcagg cggcgatcct gtcgcggcag ggaaagcatc
     1021 gcgaggcggc cgaggtgctg gagcgggccg gcagcctgta tccggcgtcg gaagaatgga
     1081 agaaggccgg cgacctcctg cgcgccgcgc gcctgatgga aaaggaaggg gagaccgatc
     1141 aggcggccga tctctacgcg cgcgccgggc atccggccga ggcggcggcg ctcatggaga
     1201 agacgcgcga tttcggcgcc gccgcgaccc tgttcaagag cgcagggcag cacgaggacg
     1261 cggcccggat gctgctgcgc gccggcgacc cgctcggcgc cgcgcggctc tcttacgagc
     1321 gcggcgatcg ggaggggtgc atccgggcgc tccaacaggt caagcgcgac agcgccgagt
     1381 tccgcgatgc ttcaatgctc ctcggaaaga tcttctcgga gcaggggctc ttcaccctcg
     1441 ccgccgacaa gttccaggcg gcgctcgacg gcgaagaggt gagcgacgag acggtcggca
     1501 tctactacgc gctcggcagg gcgcacgagg ggaacgggcg cgcccgcgag gcgctgcgcg
     1561 tctacgagtc gatcctcgcc ttcgcctacg ggcatgaaga cgtcctcggg cgggtccggg
     1621 cgctgcgctc gaagccgctg acgaccgggc cgaacccggc cccggcgcca accgaggcgg
     1681 tcgccgggcc gcgggtcgcg accgccacgt cgcgcacccg cgtggcgcca actccagggg
     1741 tcacgggcaa gtcgcctgtc ctgccgccgg gcggcgcgcg ctccgcgccc gccgccggtg
     1801 cgccggccgc ggcgcccgcc gagcggcgcc ggcctcacca cgagccggcg gccacggtcg
     1861 ctcccgccgt cgagaacggg gcggcgcgct acgtcatcgg gaagccgatc ggccgcggcc
     1921 tctccgggga agtgttccgc ggcgtcgaca gcgtcctcgg gcggccggtg gccctgcggc
     1981 gaattcacga aggaccggcg gaggccggca aggcggcgcg cctcctccag gcagcggccg
     2041 aggcggcgag tctcagccat ccgcacatcg tgccggtcta cgacaccggc caggacgcgc
     2101 gcgggccgtt catcgtcgcg gcgctcgccg aggggccgtc gctgcgctcc ctcctgcagg
     2161 cgaaggtgcg cttcgagctg caccgggtgg tggacatcgg ccggcagatc gcctcggccc
     2221 tggagcacgc ccacggccgg ggcgtcctgc accggaacct gcgccccgag aacatccaca
     2281 tcgcgccgga cgaccgggtc gccgtctcgg atttcggact gtcggtgcgg ctctccgacc
     2341 tgacgccgga ggagctgtcg agcggacgcc tcattcaata cacgccgccg gaggcgctgc
     2401 tgcgcgggcg cgtggacgtc ggctccgatc tctattcgct cggagtgatc ctgtacgagc
     2461 tggcggtcgg gcacccgccg ttccgcgggc acgacatcgg ccaccagcac gtcaacgacc
     2521 cggtgccgct gccggggccg ggggagaggc tccttccgga gttcctgaag aacgtggtgc
     2581 tgcgctgcct cgaaaaggac cgcgcgcgcc gctacccgga cgcgaagacg ctcctcgagg
     2641 acctggtgct gcgggagatc gtccccggga tggtcgtctc gggccggtac caggtgctcg
     2701 cggagatcgg ccgcggtggc atgggagcca tcttccgggc ccgcgacgcc gagctggacg
     2761 aaaccgtggc gatgaaggtg ctggcggccg acatcgacgc cgagaccgcc gcccgcttcg
     2821 tgcaggagat caagatcgcc cgctcgatcg tccacccgaa cgtcgtgcgt gtgcacacgt
     2881 tcgagaagtg gcgcgacttc cggctgctga tgatggagta catcgacggc gtgccgctgc
     2941 cgcgctggat ggagcgggtg ccgcggccat cgcgcgccga ccggctgcac gtggcgatgc
     3001 aggccgcggc cggcctcgag gcggcgcacc gcgccggcat cgtccaccgc gacatcaagc
     3061 cggagaacat cctcgtcacc ggcgccgggc aggcgaaggt gctcgacttc ggcatcgccc
     3121 ggcccgagtc ggccggccac acgctcaccg agaaagggct cgtcctcggc acgccgcgct
     3181 acatgtcccc cgagcaggtg caggggaagc cgctcgaccg gagaagcgac atctactccc
     3241 tgggggccgt gctcttcttc ctcttcaccg gcgaggagcc gttcgtcggt tcggacgtgc
     3301 gcgagacgct cctcacgcac ctgcggcccc ccgtcaagac accgctcaac atcgacccgt
     3361 cgcttccggt gccgctgtcg caggcgatcc tgaaggcgct cgaggtcgat ccggacggcc
     3421 gcttcccgac cgccgacgcg ttcgccgcag ccctcgcccg cgccgtcgag ccgcgcgccg
     3481 catagccatc agtcgccggc gaaacgcagc gacccgaaca gaccatgcgt ctcgtcgtcg
     3541 ggaccggcgg cgaaatagag agtgtcggca tcgccggccg tgaccccgtt gccgaagagg
     3601 atcccccaca ggccctcgat tgcgatcggc ctggcatcct cgcctctcat gaagccaagg
     3661 aattcgccgg tctccgggtc gaaggcgagg atacgcccgg tggcgaaatc gccgaccagg
     3721 aggtcgttgc tgaacggacc gaagtcggca ggcgccagcg ccatgcccca gggcgccacg
     3781 aggcgcccgc cgtggccgat cagctgctgc agcaggtgac cgtcggtgtc gtacacgttc
     3841 acggcgccgg gaccgtggct ggtctcctct tcgggatccc gcgcgctgtg ccgcgcgtag
     3901 gcgatgtaaa gcctgccgcc gatgttctgg atgttgaacg gcgaaaggct tctgccgaga
     3961 tcggggtcgg tgaacgagcc atcgagctcg gtgagatcga aatcggcgtc gaacacgtcg
     4021 atcttcccgt tctcgaaatc ggcggcgtag atcacgttcg ccccggtcga ggtctgcccg
     4081 atggtcatcc ccgtgtaggt cgcctccgcg acggctgcga cctgcgccgt ggtggacggc
     4141 ggcgggggag gcacggccgg gttccagccg gtgacctggc cacccaggga gacgaacagg
     4201 aagagagctg ggccgctgtc cgcccccgcg ctgacgacga agtccgcagt gggattgaag
     4261 gcggtcccgg tcggcacccc gccggggatg gccacggtga gcgcggcctt cgagaaggtg
     4321 ctgccttcga cgtcaccggt gtagagtgtg gcggtcccgg tttcgttgtt cgcaacccag
     4381 aacgcgcccc cggccggact cagcgagatt ccccagggat tcacgagatc cgggtcctgg
     4441 atgagggcga cgcccggctc gtcggagacc aggttggtct gctcgtagaa gccggcgctt
     4501 tgcggcgcgg cagtgccgtt cggagcggca gggaggccgg tggccgatgc cgccgacagc
     4561 agcgcgatcg ccgcggcagc gatcgcccga aggggagttt gcacggtcga aggattcatg
     4621 gtggacctcc attgtggttc agtcccgcga ggacggttcc ggcgtgaccg atggttttga
     4681 gttactagaa atcctatcgc gggctgaaga tcgcgtctgt aaggtaaatc accgaggcca
     4741 gcgaccgcga tg
//
LOCUS       DASRIQ010000421         9301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3281049, whole genome shotgun sequence.
ACCESSION   DASRIQ010000421 DASRIQ010000000
VERSION     DASRIQ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3281049"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2756
                     /locus_tag="VFD06_10270"
     CDS             <1..2756
                     /locus_tag="VFD06_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03957.1"
                     /translation="LGFIPQYVAAFSEPLADRMVIPLDLPPDELYNLFGHELVHVFQF
                     SMLYQESVSRAFRSNAPGWVMEGMASYLAQDESSLDQMIIRDAVIYGLIPPIHRVQVT
                     TFLTYRFGHAAFAFIEEKYGQEGLRTFIAEFRKSLLTNNIEKPIQDAFGIDGDEFDRQ
                     FKKYLQKKYLPFLLEKKEPEDYGREIARKNPKDAEVVNFSPALSPSGDLLAVLTTRWG
                     DLDAAILSARDGKVWRPLTKGFTNKYESLVYGAFQGKKDLTWSPDGLEVAFFARRENE
                     RVLLIHSAVDGDLKARISMPGIDDEISPSWSPDKDIIAFEGVKDGVTDIFTYHLKTKE
                     IRNLTQDTFFDSNPAWSQDGKQVLYNRRIGPATKIFMVDAGDPTRKVQLTFGESEDLM
                     PTFSRDGKTVYYTSDVDGGIYNLHGLSLETGDITRLTSLAGGAFTPVELPDEGGKSSL
                     AFSAYTEGRLRIYRMQPGEPEGVKRAAEQSQEPADLQPFEPPLRLTLDEGAKKPYDKI
                     HFHLENAPSVLVGVADDGTLLGNAQVLLSDLLGDNRMLFDFQSVSTFSNFYFQYSNLK
                     RRMNWGAFVRDYRDYYLVSSAFGDTARVQQTTSVTGGGVNISYPFSRYYRIGASAGYF
                     FRTADRPYFNGETTVFESLDENFPYIGWQLNGDTVRYKEFGPYHGQRFQLSQQWAPTM
                     NSSGSTDLFTTGPFLNTLIDYRVYRRVTDRSLFAIRLASYLSQGDGYSVYSFGGLNYL
                     RGYDYREFYGSRVAYANIEFRFPLVDALVFPIGILRDLRGFAFFDVGTAWFQDDEFSH
                     QAFGFNLTDAANGVVNTDRNTIVNFFDPTTGQLLRRKFSFWDDENDKLGDGRAAYGFG
                     FNVFLGPFQLTWSYAHQLDNTIEVCDLTGDGVCNPDTDIKRLDDPFNESGSIGQFYIA
                     IDY"
     gene            complement(2786..3679)
                     /locus_tag="VFD06_10275"
     CDS             complement(2786..3679)
                     /locus_tag="VFD06_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN03958.1"
                     /translation="MTAWVLPVLREARDGLTRHPALTALATLSMAVSLYVLGMFLLIA
                     WNVRLTTTALGRELQMQVYMRPGADAEAIETIRQGLRSDDAVAEARLVTADEARRRFA
                     ERFPRLAGLSEGLGPDLFPTSFEVVLRESHRGPEEAERLAKAYRVGPGVDEIRFDRGW
                     FERLTALFDLFRSGGYGVGALLVLAVMVTVGAIVRLTVLARREEIEIMKLVGATAAFI
                     RAPFLMGASAQGLVGGLLALGMLRLSWELLVRSEPYRENAMLALAAGRFPPASLMLLL
                     PALGMLLAAAAAALSLRRASA"
     gene            complement(3705..4373)
                     /gene="ftsE"
                     /locus_tag="VFD06_10280"
     CDS             complement(3705..4373)
                     /gene="ftsE"
                     /locus_tag="VFD06_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZN03959.1"
                     /translation="MIQLFHVTRRYGSGAPALSDISFSIEKGDFVFLTGPSGAGKTTL
                     LRLLFRDDLPTSGQVMVNNENLASMPPSRVPYLRRQIGVVFQDFKLLRRKTVFENVSY
                     ATQVIGLPAKEQRRRTYQVLEMLALNHRLHHFPEQISGGEQQRVAIARALVNRPLVLL
                     ADEPTGNLDPDLSVAILDTFKEVNAQGTTVLVATHDRALIRRMARRVLTLSQGQLIPG
                     GAAA"
     gene            complement(4390..5850)
                     /gene="gatB"
                     /locus_tag="VFD06_10285"
     CDS             complement(4390..5850)
                     /gene="gatB"
                     /locus_tag="VFD06_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZN03960.1"
                     /translation="MSNPLDRWEPVIGLEVHCQLLTDTKIFCGCPTKFGAPPNTQVCP
                     VCLGMPGVLPVLNRRAVEYAVRAGLALHCDIQPTSIFARKNYFYPDLPKGYQISMYDR
                     PLMTGGWFEIDPGTGAGPRKVRLTRIHMEEDAGKLLHEGFADSATRSYVDFNRSGMPL
                     IEIVSEPDMRSPQEAHAYFSMLRAALVWIGVTDGNMEEGSLRCDANVSLRPRGQQKFG
                     TKVELKNLNSFKFVQFGIEHEIARQAEVLDGGGTIVQETRLYDPKTDRTHAMRSKEEA
                     HDYRYFPEPDLPPLVVTNAWVEEIKKDLPELPWDRRARYVGPLGIKEQDARVLTSDRA
                     LGDYFEAAARASGAPKAAASWVLTEVLRKMKEGEGSIGDAPLPPERLGALLKLVESGS
                     ISGKIAKELFEEIWTSGADPAALVAERGLTQVSDEGAIAAAVDAVIAASTAQVASYKG
                     GKTGVLGFLVGQVMKSTGGKANPALVNRLLKERLDR"
     gene            6038..7060
                     /locus_tag="VFD06_10290"
     CDS             6038..7060
                     /locus_tag="VFD06_10290"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZN03961.1"
                     /translation="MASVRVAILGAGNLGTALGIVLTGGSPGIRAGRNRHVSLWTIEE
                     DVAEDIRVRRQNRKYLPDVELPRGLHVTLDLREALSGADVVIFALPSKVVREVARQVS
                     AAYAAATPVPKPAIVSSSKGLEAETDRRMSQVIAGEMPEGLGIPVMAMSGPSIAHELS
                     RGVPTAVTLAHADPRQARAVRRQIQTPILRFDISRDVAGVELGGVLKNAYTLLFGLCD
                     GLGLGLNTKAALLTRALPEMIRLGVALGGRRATFLSLPGLGDLVGTGLSHHSRNRRMG
                     EELVRRSHDDALASVSGVVEGVGSLALARALAAKRRMKLPILDGIAAILDHKADPLAT
                     IQKLAG"
     gene            7085..>9301
                     /locus_tag="VFD06_10295"
     CDS             7085..>9301
                     /locus_tag="VFD06_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HZN03962.1"
                     /translation="MNAHRGRKLAWLLAGLFALAGRQALAQAPPAAASSPPAPAAPSV
                     PTTEIVVTASPVSTQADLESIFDQIYTIGLDTERPLAVAGLKIKRDTLELTLNQGTVW
                     LAKPIAGQITGAYFSGNATIRMTIPAAIDRKQFEAAIGKPSIDMSIREVVLRFDDGAE
                     KEILAAAKPATGAAAPGTTWNERLLVDSLSGDATPMDFLDTRINTLKHTTLFTADFPG
                     ADGKTWYRYVHNGAERIEDSFYREEFAGAGGKRDYRIIARFHRPEDYDAKGNYDLMPE
                     SDAKEVALVRNVEMTVEIPNTKSVSIDAKLTVEALRDNIRAVRFDFVNNLDNSGEWKA
                     TGRPVTPTHVADSSDSRVPYLHRWHELLVLLPKPLAKGERTVVHVKAMEDTIIQLTDK
                     SYWIYTTYPWFPQIGYLGGRYTMDWTVKIAKPMQAAGTGDLVKEWEEGGLNCSRWKSD
                     VPVQFASFIFGSFKVTPGSYKRQPPGTGEIPIRIFTILGGEQHSKANPESVIYNIGEG
                     IKTYETIFGPFPYQQLDVAEMAKYMGFAQAPAGVLLVSSVQVGGTALADERGVYAVNI
                     EEQGGLGKTGGGGTGDQFVFHELAHQWWGHQVGWVGDEDQWFSEAFAEYSAALMVTAI
                     DPKRFEDMRKRWRQGAIDGDPWGTLSTANTSETREHPGTRWNMVYAKGPYVVHMLRTW
                     MGWEKFTQYTTTLQTKYKNTSINNDTLAREAGKVMGYSMFPFFDQWVRDKGIPRVHY"
BASE COUNT         1569 a         3220 c         3118 g         1394 t
ORIGIN      
        1 cgctcgggtt catcccgcag tacgtcgccg ccttctccga gccgctcgcc gaccggatgg
       61 tcatcccgct cgacctcccg ccggacgagc tgtacaacct gttcggccac gagctggtgc
      121 acgtcttcca gttcagcatg ctctaccagg agtcggtgtc gcgcgccttc cggagcaacg
      181 cccccggctg ggtgatggag gggatggcct cgtacctggc gcaggacgag tcgagcctcg
      241 accagatgat catccgcgac gcggtgatct acgggctcat cccgccgatc caccgcgtgc
      301 aggtgacgac cttcctgacc tatcgcttcg gccacgccgc cttcgccttc atcgaggaga
      361 agtacggcca ggagggcctg cggaccttca tcgccgagtt ccgcaagagc ctgctgacga
      421 acaacatcga gaagccgatc caggacgcct tcgggatcga cggggacgag ttcgaccggc
      481 agttcaagaa gtacctccag aagaagtacc tgccgttcct gctggagaag aaggagccgg
      541 aggactacgg ccgggagatc gcccggaaga acccgaagga cgcggaggtg gtgaacttca
      601 gcccggccct ctccccctcc ggcgatctcc tcgcggtcct gacgacccgc tggggggacc
      661 tggatgcggc gatcctctcc gcgcgcgacg gcaaggtgtg gcggccgctc accaaggggt
      721 tcaccaacaa gtacgagagc ctggtgtacg gcgccttcca ggggaagaag gacctgacct
      781 ggtcccccga cggcctcgag gtcgccttct tcgcccgccg cgagaacgag cgcgtcctgc
      841 tcatccacag cgccgtggac ggcgatctca aggcgcgtat ctcgatgccc gggatcgacg
      901 acgagatctc cccctcctgg tcgccggaca aggacatcat cgccttcgag ggggtgaagg
      961 acggggtcac cgacatcttc acctaccacc tcaagacgaa ggagatccgc aacctgaccc
     1021 aggacacctt cttcgacagc aacccggcgt ggtcgcagga cggcaagcag gtcctgtaca
     1081 accgccgcat cggaccggcg acgaagatct tcatggtcga tgcgggcgat ccgacccgca
     1141 aggtgcagct gacgttcggg gagagcgaag acctcatgcc gaccttctcg cgcgacggca
     1201 agacggtcta ctacacgagc gacgtcgacg gcggcatcta caatctgcac ggcctgagcc
     1261 tcgagaccgg cgacatcacg cggctcacga gcctcgccgg cggagcgttc acgccggtgg
     1321 agctgccgga cgagggggga aagtcgtcgc tcgccttctc cgcgtacacc gaagggcgcc
     1381 tgcgcatcta ccgcatgcag ccgggggagc cggaaggggt gaagcgggcc gccgagcagt
     1441 cccaggagcc ggccgatctg cagccgttcg agccgccgct ccgcctcacg ctcgacgagg
     1501 gcgccaagaa gccctacgac aagatccact tccacctcga gaacgccccc tcggtgctcg
     1561 tcggcgtggc cgacgacggg acccttctcg ggaacgccca ggtgctgctg tcggacctgc
     1621 tcggcgacaa ccggatgctg ttcgacttcc agtcggtgtc caccttctcc aacttctact
     1681 tccagtactc gaacctgaag cgccgcatga actggggggc gttcgtccgc gactaccggg
     1741 actactacct cgtctccagc gcgtttggcg acacggcgcg ggtgcagcag acgaccagcg
     1801 tcaccggcgg cggggtgaac atctcgtacc ccttcagccg ctactacagg atcggcgcct
     1861 cggccgggta cttcttccgc actgccgacc ggccctactt caacggcgaa acgacggtgt
     1921 tcgagtcgct ggacgagaac ttcccctaca tcggctggca gctgaacggc gacacggtgc
     1981 gctacaagga gttcgggccg taccacgggc agcggttcca gctgtcgcag cagtgggcgc
     2041 caaccatgaa ctcctccggg tcgaccgacc tgttcacgac gggcccgttc ctgaacacgc
     2101 tgatcgacta ccgcgtgtac cggcgggtca ccgatcgatc gctgttcgcc atccggctcg
     2161 cctcgtatct cagccagggg gacgggtaca gcgtctattc gttcggcggg ctcaattacc
     2221 tgcgcggcta cgactaccgc gagttctacg gcagccgggt ggcatacgcc aacatcgagt
     2281 tccgcttccc gctcgtggac gcgctggtct tcccgatcgg catcctgcgg gacctgcgcg
     2341 ggttcgcctt cttcgacgtc ggcaccgcct ggttccagga cgacgagttc tcgcaccagg
     2401 cgttcggttt caacctcacg gacgccgcga acggggtcgt caacacggat cgcaacacca
     2461 tcgtcaactt cttcgatccg acgaccggcc agctcctccg gcggaagttc agcttctggg
     2521 acgacgagaa cgacaagctc ggagacgggc gcgctgccta cggcttcggg ttcaacgtct
     2581 tcctcggacc gttccagctc acctggtcgt acgcgcacca gctcgacaac accatcgaag
     2641 tgtgtgacct caccggcgac ggcgtctgca acccggacac ggacatcaag cgactggacg
     2701 acccgttcaa cgagagcggg tcgatcggcc agttctacat cgccatcgac tactgagcga
     2761 ttggcggaga caccgcggcg gcggcctagg ccgaggcgcg tcgcagggac agggccgccg
     2821 cggcggcggc gagcagcatc cccagcgccg ggagcaggag catcagcgac gcggggggga
     2881 agcgcccggc ggcgagcgcc agcatcgcgt tctcccggta cggctcggag cgcaccagga
     2941 gttcccagct cagccgcagc atcccgagcg ccagtagccc cccgacgagc ccctgcgccg
     3001 aggcccccat caggaacgga gcgcggatga aggcggcggt cgcgccgacg agcttcatga
     3061 tctcgatctc ctcgcggcgc gctaggacgg tgagacggac gatggcgccg accgtcacca
     3121 tcaccgccag gaccagcagg gcgccgacgc cgtagccgcc gctgcggaac agatcgaaca
     3181 gcgcggtcag ccgctcgaac cagccgcggt cgaagcggat ctcgtccacg ccggggccga
     3241 cccggtacgc cttcgccagc cgctccgcct cctccggccc ccgatgcgac tcccgcagga
     3301 cgacttcgaa cgaggtcggg aagagatccg gcccgagccc ttccgacagc cccgccagcc
     3361 gcgggaagcg ctccgcgaag cggcggcgcg cctcatccgc cgtcaccagg cgcgcctcgg
     3421 cgaccgcgtc gtcgctccgg agcccctggc ggatcgtctc gatcgcctcc gcatccgccc
     3481 cggggcgcat gtacacctgc atctgcagct cacgcccgag cgccgtggtg gtgagccgga
     3541 cgttccacgc gatgagcagg aacatcccca gcacgtacag ggacaccgcc atcgacagcg
     3601 tcgcgagcgc cgtcagggcc gggtgccgcg tcagcccgtc gcgcgcctcg cgcagcaccg
     3661 ggaggaccca cgcggtcacg ccgtcccccg ggccgcccgc gacctcacgc cgccgccccg
     3721 cccgggatca gctgcccctg cgacaacgtc aggacgcgcc gcgccatgcg ccggatgagg
     3781 gcgcgatcgt gcgtcgccac caggacggtc gtcccctgcg cgttgacctc cttgaaggtg
     3841 tcgaggatgg cgaccgagag gtcgggatcg aggttgccgg tcggctcgtc cgcgaggagc
     3901 acgagcggcc ggttgacgag cgcccgggcg atcgccacgc gctgctgctc cccgccggag
     3961 atctgctccg ggaagtggtg cagccggtgg ttcagcgcca gcatctccag gacctggtag
     4021 gtgcgtcggc gctgctcctt cgccgggagc ccgatcacct gggtggcgta ggagacgttc
     4081 tcgaagacgg tcttgcggcg caggagcttg aagtcctgga agacgacgcc gatctgccgg
     4141 cgcaggtacg gcacgcgcga cggcggcatg ctggcgaggt tctcgttgtt gaccatcacc
     4201 tggccggacg tcggcaggtc gtcgcggaag aggaggcgca ggagggtcgt cttcccggcg
     4261 ccgctgggcc cggtcaggaa gacgaagtcc cccttttcga tcgagaagga gatgtccgac
     4321 agggccggcg ccccggaccc gtagcggcgg gtcacgtgga agagctggat catcccggtc
     4381 ccgccggact caccggtcga gacgctcctt gagcaggcgg ttgaccaggg cggggttcgc
     4441 cttcccaccc gtgctcttca tcacctggcc gacgaggaac cccagcaccc ccgtcttgcc
     4501 tcccttgtac gaggcgacct gcgcagtgct ggcggcgatc accgcgtcca cggcggcggc
     4561 gatggcgcct tcgtcggaga cctgggtgag gccgcgctcg gccacgagcg cggccgggtc
     4621 ggcgccgctc gtccagatct cctcgaacag ctccttggcg atctttccgg agatgctgcc
     4681 gctctcgacg agcttcagga gggcgccgag gcgctctggc ggcagcgggg catccccgat
     4741 ggatccctcc ccttccttca tcttgcgcag cacctcggtg aggacccacg aggcggccgc
     4801 cttcggcgct cccgaggccc tggcggcggc ttcgaagtag tcgccgagcg cgcgatcgct
     4861 cgtcagaacc cgcgcgtcct gctccttgat gccgagaggg ccgacgtagc gggcgcggcg
     4921 gtcccacggc agctccggca ggtccttctt gatctcctcg acccatgcgt tcgtgacgac
     4981 gaggggcggc aggtcgggct cggggaagta gcggtagtcg tgcgcctctt ccttcgagcg
     5041 catcgcatgg gtccggtcgg tcttcgggtc gtagaggcgg gtctcctgga cgatggttcc
     5101 tcccccatcc aggacttccg cctgccgggc gatctcgtgc tcgataccga actggacgaa
     5161 cttgaaggag ttgaggttct tcagctcgac cttggtgccg aacttctgct gcccgcgggg
     5221 gcggagcgac acgttggcgt cgcaacgcag cgacccttcc tccatgttgc cgtcggtgac
     5281 gccgatccag acgagcgcgg ctcgcagcat cgagaagtac gcgtgcgcct cctgcggcga
     5341 gcgcatgtcc ggctcggaga cgatctcgat gagcggcatc ccggagcggt tgaagtccac
     5401 gtacgaccgg gtcgcggagt cggcaaagcc ttcgtgcagg agcttgccgg catcctcctc
     5461 catgtggatg cgggtgaggc gcaccttcct gggtccggcg ccggtgcccg ggtcgatctc
     5521 gaaccagccg ccggtcatga gaggccggtc gtacatcgag atctggtacc ccttaggcag
     5581 gtccggatag aagtagttct tgcgcgcgaa gatcgacgtg ggctggatgt cgcagtgaag
     5641 cgccagcccg gcgcggacgg cgtactcgac cgccctacgg ttgagcaccg gcagcacccc
     5701 cggcatgccg aggcagaccg ggcagacctg ggtgttcggc ggggcgccga acttcgtcgg
     5761 gcagccgcag aagatcttgg tgtcggtcag gagctggcag tggacctcca gcccgatgac
     5821 cggctcccat ctgtcgaggg ggttcgacat actcgtcgcg gtggcgcggc gcgcctctgc
     5881 agcccgcccg ccgggccgcg gattatagca cccgcctcct ggcggtccct gccgccccgt
     5941 cgcccggacc ggccccgccg ggcccgcggc gggccccccg gagcgctgtg ctacagtacc
     6001 gcggccggag ggctttccgg ccccggagac agcaggcgtg gccagcgtgc gcgtcgccat
     6061 cctcggcgcc ggcaatctcg gaacggcgct cggcatcgtc ctcacgggcg gcagccccgg
     6121 cattcgcgcc gggcgcaacc ggcacgtcag cctctggacc atcgaagagg acgtcgccga
     6181 ggacatccgc gtccgccgcc agaaccgcaa gtaccttccg gacgtcgagc tgccgcgcgg
     6241 cctgcacgtc acgctcgatc tcagggaggc gctgtcgggg gcggacgtcg tgatcttcgc
     6301 gctcccatcg aaggtggtgc gggaggtggc gcggcaggtc agcgcggcct atgccgcggc
     6361 gaccccggtg ccgaagccgg cgatcgtgtc ctcgtcgaag gggctggagg cggagaccga
     6421 ccggcgcatg agccaggtga tcgccgggga gatgccggag gggctcggga tcccggtgat
     6481 ggcgatgtcg gggccgtcga tcgcgcacga gctgagccgc ggcgttccga ccgccgtcac
     6541 cctggcacac gccgaccccc gtcaggcgcg cgcggtgcgg cgccagatcc agacgccgat
     6601 cctgcgcttc gacatcagcc gcgacgtcgc cggggtcgag ctgggcgggg tgctgaagaa
     6661 cgcctatacg ctcctgttcg ggctgtgcga cggcctcggc ctcgggctca acaccaaggc
     6721 ggcgctcctg acgcgggcgc ttccggagat gatccgcctc ggcgtggccc tcggcgggcg
     6781 gcgcgcgacg ttcctcagtc ttcccggcct cggcgacctg gtgggcaccg gcctcagcca
     6841 ccacagccgg aaccggcgca tgggtgagga gctggtgcgc cggtcgcacg acgacgcgct
     6901 ggcgtcggtg tccggcgtgg tcgagggggt cggctcgctc gcgctcgccc gcgccctcgc
     6961 cgccaagcgc cgcatgaagc tgccgattct cgacgggatc gcggcgatcc tcgaccacaa
     7021 ggccgatccg ctcgcgacca ttcagaagct cgcggggtag aatcaaacat ccggggactc
     7081 cgacatgaat gcacatcgag gtaggaagct ggcgtggctt ctcgcaggac tcttcgcgct
     7141 cgccgggagg caggcgctgg cgcaggcgcc cccggcggcg gcatcgtccc ccccggcacc
     7201 ggccgctcct tccgtgccga cgaccgagat cgtggtcacc gcatcaccgg tctcgaccca
     7261 ggccgacctc gaatcgatct tcgatcagat ttacaccatc gggctcgaca cggagcgtcc
     7321 cctcgcggtg gcggggctca agatcaagcg ggacacgctg gagctgaccc tcaaccaggg
     7381 gacagtgtgg ctcgcgaagc cgatcgccgg ccaaatcacc ggcgcgtatt tcagcggcaa
     7441 cgccaccata cggatgacga tcccggcggc catcgaccgc aagcagttcg aggcggcgat
     7501 cggcaagccg tcgatcgaca tgtccatccg cgaggtggtg ctgcgcttcg acgacggcgc
     7561 cgagaaggag atcctcgccg ccgccaaacc ggcgaccggc gcggccgccc ccgggacgac
     7621 atggaacgaa agactccttg tcgattcgct gtccggcgac gccaccccga tggacttcct
     7681 cgacacgcgg atcaacaccc tcaagcacac gaccctgttc acggccgatt tccccggcgc
     7741 cgacgggaag acctggtacc gctacgtcca caacggcgcg gagcgcatcg aggacagctt
     7801 ctaccgcgag gagttcgcgg gcgcgggcgg gaaacgggac taccggatca tcgcgcgctt
     7861 ccaccgtccg gaggactacg acgccaaagg gaactatgac ctgatgcccg agtccgacgc
     7921 caaggaggtc gcgctcgtcc gcaacgtcga gatgaccgtc gagatcccca acacgaagtc
     7981 cgtgtcgatc gatgcgaaac tgaccgtcga ggcgctgcgt gacaacatcc gcgcggtccg
     8041 cttcgatttc gtcaacaacc tcgacaactc gggcgagtgg aaggccacgg gacgaccggt
     8101 gacccccacc catgtcgccg actcgagcga ctcgcgggtc ccgtacctgc accggtggca
     8161 cgagctgctc gtcctgctgc cgaagccgct cgcgaagggg gaacggaccg tcgtgcacgt
     8221 caaggcgatg gaagacacga tcatccagct caccgacaag tcctactgga tctacacgac
     8281 gtatccctgg ttcccgcaga tcggctacct cggcgggcgc tacacgatgg actggacggt
     8341 gaagatcgcc aagccgatgc aggccgcggg aacgggggac ctggtcaagg agtgggagga
     8401 gggcggcctc aactgctcgc gctggaagag cgacgtcccg gtccagttcg cctcgttcat
     8461 cttcgggtcg ttcaaggtga ccccgggatc ctacaagcga cagccccccg gcaccggcga
     8521 gatcccgatc cgcatcttca cgattctcgg cggcgagcag cactccaagg cgaaccccga
     8581 gagcgtcatc tacaacatcg gggaagggat caagacctac gagacgatct tcggcccgtt
     8641 cccgtaccag cagctcgacg tcgccgagat ggcgaagtac atggggttcg cacaggcgcc
     8701 ggccggggtc ctgctggtca gcagcgtgca ggtgggcggc acggcgctgg cggacgagcg
     8761 cggcgtgtac gccgtcaaca tcgaggaaca gggagggctc gggaagaccg gcggcggcgg
     8821 cacgggggac cagttcgtgt tccacgagct ggcgcaccag tggtgggggc atcaggtggg
     8881 ctgggtcggt gacgaggacc agtggttcag cgaggcgttc gccgagtaca gcgccgccct
     8941 catggtgacg gcgatcgacc cgaagcggtt cgaggacatg cgcaagcgct ggcgccaggg
     9001 ggccatcgac ggggacccgt gggggacgtt gagcaccgcc aacacctccg agacccgcga
     9061 gcacccggga acgcgctgga acatggtgta cgccaaggga ccgtacgtcg tgcacatgct
     9121 gcgcacctgg atggggtggg agaagttcac ccagtacacg acgacgctgc agacgaagta
     9181 caagaacacc agcatcaaca acgacaccct cgcccgcgaa gcaggaaagg tgatgggcta
     9241 cagcatgttc cccttcttcg atcagtgggt tcgcgacaag gggatcccga gggtccatta
     9301 c
//
LOCUS       DASRIQ010000422         6473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2498386, whole genome shotgun sequence.
ACCESSION   DASRIQ010000422 DASRIQ010000000
VERSION     DASRIQ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498386"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFD06_10300"
     CDS             complement(<1..566)
                     /locus_tag="VFD06_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZN03963.1"
                     /translation="MLSIKKSQRLFFEAFEGMARGAVDAAGILESLVGSGRDQMESHA
                     ARIKTIEHSCDRIVHDLVRELNRTFITPFDREDIHDLGTAQDDIVDLIDATASRAVLY
                     GVGGEVPEAAGLAGVIKRQAGEILVAVSQLKEPEHILEQCKRIKDMETEGDRLYREAM
                     KRIFGGTPDPIFVITAKEIIEMLEATTDA"
     gene            801..1151
                     /locus_tag="VFD06_10305"
     CDS             801..1151
                     /locus_tag="VFD06_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03964.1"
                     /translation="MIPMPDSGGSGEVTKRREAPALLSLIDVVAATQDSVLPPGKRDS
                     SGFWYPASCRNPTELFTPYQRSLQEKQRSVHRERRRRVASSRVRSSDPAPEQSPLGDR
                     PVPMPLPCPEAPEP"
     gene            1233..2693
                     /locus_tag="VFD06_10310"
     CDS             1233..2693
                     /locus_tag="VFD06_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN03965.1"
                     /translation="MTTSLRKSLSPARLFVDGQWVDPVSGQSFKTINPATEEPITDVA
                     DASAEDVDRAVQAARRAFESGPWSKMPAADRGRILWKIGDLIEANVQELGRLETLDQG
                     KTITESTRVDVPLAADCFRYFAGWTTKIEGETIPVRAPSLNYTLREPLGVIGAITPWN
                     FPILMAAWKIAPALAAGNTVVLKPAKETPLTALVLAELAIEAGLPPGVLNVVPGRGTV
                     AGAALVEHPHVAKIAFTGSTEVGRDIMRRGSDSLKHLTLELGGKSPNVVFADADLDQA
                     VRGASNGIFYNKGEVCTAGSRLFLEESVHDAFLDRLQSHVGKLQQGDPLDPKTRLGPQ
                     VSEAQMRQILGYVDKGKAEGARLVTGGERPAGKGFFVRPTIFTEVRNDMTLAREEIFG
                     PVLAVLRFKDLDEVLAQANDSVYGLAAAVWTRDIKRAHQTARRLRAGTVWINSYGLYD
                     NAMPFGGVKQSGFGRELGRQGLLEYTQTKSVWVDLS"
     gene            2706..3494
                     /locus_tag="VFD06_10315"
     CDS             2706..3494
                     /locus_tag="VFD06_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN03966.1"
                     /translation="MPEPLVRAHADAGVLTLTLDAPPANCYSYDLMRELDACVLAARM
                     DPSVDVIVLRGAGDRFFCAGADIRMLESVTPEFKYAFCLHANETLRRLEETPKLVIAA
                     LNGHAVGGGLEVAMACDLRLAKKGGGKIGLPEVSLGVLPGTGGTQRLLRLVGRAKAIE
                     LMATGGTFSFDEALGMGLVHRVFEADGFDAEVTRYARQFCPPGKASLAVGLIKRAVVT
                     GAELPLAEALALERELQQRLFTSEDAREGLAAYNAKRPPRFRGR"
     gene            3545..4573
                     /locus_tag="VFD06_10320"
     CDS             3545..4573
                     /locus_tag="VFD06_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN03967.1"
                     /translation="MRAVVVPRHGGPEVLEVAERPRPVPGPGEVLLQVKAAGVNHLDT
                     FVRRGMPGITLPLPMILGSDAAGTIAAVGAAVMGIKEGDRIFVSPGYSCERCRACLSG
                     NEPLCRFYGLLGEHRDGTQAEYVALRATQALPLPDGVSFEEGAAFPLVFLTAWHMLVA
                     RAGLRPGEEVLVHAGGGGVGSAAIQIAKLHGARVLTTVGGPEKAAKARALGADEVIDH
                     RSTDVLAEVRRLTSKRGVDVVVENVGQETWEKSLLALAKGGRLVTCGATTGYEAKTDL
                     RHLFFKGLSLLGSTMGSRAELLQIADLVGARRLRPIVDRVLPLEQAAEAHRLLAARSV
                     FGKIVLSP"
     gene            4583..5452
                     /locus_tag="VFD06_10325"
     CDS             4583..5452
                     /locus_tag="VFD06_10325"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN03968.1"
                     /translation="MSSSTVVDAHLHIQPWSQFRPEAIRTMEKRADMETVRAVCDDPK
                     ALLTFLDREGIDRAVLVNYVAPDVIGFTDEVNTFIGRYCRGHEDRLVPVGGVHPRLSA
                     DPAGDLEHIVTRHGVRVLKLHPPHQLFRMNAYRDGGDLPSLAGLYERAAALGVPILVH
                     TGTSVFPGARNKYGDPMDLDDLAVDFPRLTIIMAHAGRPLWMEACLFLLRRHPNVYVD
                     LSGIPPKNLLGYFPRLAEVAHKAMFGTDWPGPGVPGVRQNLEAFRALPLPEEARRAIL
                     SGTADRVYPARRP"
     gene            5463..>6473
                     /locus_tag="VFD06_10330"
     CDS             5463..>6473
                     /locus_tag="VFD06_10330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN03969.1"
                     /translation="MEHAEVLFIDRLPDRAPPLRGEARADLAIAGGGLTGLATALAAR
                     ARFPKRRIVLLEKDRCGTGASGRNGGMALPGTALDLEDLEGRLGFDRARRVSAWLARG
                     LAILMERARASGAAAGPIEEVGTLSLARTPRHARAQRRRLEVYRRHGVEAEILEGSAL
                     ERAFRSPLYRSAFHVLRGGALVDPWRLTTALRRRALDAGVVIHEESEVRSIEEGPEIR
                     LTTPLGRLTARALVLATNAFSPKLGFFRSRVAPVHVSCIATSPLSRNVRAAIGWEGRQ
                     AAWEEGRVYHFLRLTADDRVLLGGGNIDYRLGGRTRFDPSRRHAKLMRSLRAIFPPLK
                     DVP"
BASE COUNT          894 a         2334 c         2286 g          959 t
ORIGIN      
        1 gcgtcggtcg tcgcctcgag catctcgatg atttccttcg ccgtgatgac gaagatcggg
       61 tcgggcgtcc cgccgaagat ccgtttcatc gcttcgcggt agagccggtc accctcggtc
      121 tccatgtcct tgatccgctt gcactgctcg aggatgtgct ccggctcctt gagctgcgac
      181 acggcgacga ggatctcccc cgcctggcgc ttgatgaccc cggcgagccc ggccgcctcc
      241 ggaacctcgc cgccgacgcc gtacagcacg gcccggctgg cggtggcgtc gatcaggtcg
      301 acgatgtcgt cctgcgccgt cccgaggtcg tggatgtcct cgcggtcgaa cggcgtgatg
      361 aaggtgcggt tcagctcgcg cacgagatcg tggacgatcc ggtcgcagct gtgctcgatc
      421 gtcttgatgc gcgcggcgtg cgactccatc tggtcgcgcc cggagccgac cagcgactcg
      481 aggatgccgg ccgcgtcgac cgcgccacgc gccatcccct cgaacgcctc gaagaacagg
      541 cgctggcttt tcttgatgct cagcatcgtc cctcccgcca cagccgcgcg ggtggcgcgg
      601 ccatggtgac gatcgcgtgt cgttcgggtg acagggcgcg cgaacaatat ccagcgccac
      661 gacgactgtc aacgacggcc ctgggaaaac ggatctcgct tcgcgcgcaa tgtccgcatt
      721 gagcgggatt gcgctcggcg cgcccctttt gttagcatgt gaatggctcg ttttagatgg
      781 tgcaaccgaa ggctcacggg gtgatcccga tgccggactc cggggggagc ggcgaagtca
      841 ccaaacggcg cgaggcgccg gcgctcctgt ccttgatcga tgtcgtcgcc gcgacgcagg
      901 actcggtcct cccgccgggg aagcgcgact ccagtggctt ctggtacccc gcctcctgcc
      961 gcaacccgac ggagttgttc accccgtacc agcgctccct gcaggaaaag cagcgcagcg
     1021 tccatcgcga gcgtcgccgg cgcgtcgcct cctcccgcgt ccgttcgtcc gacccggcgc
     1081 cggagcagtc gccgctgggc gatcgcccgg tgccaatgcc tctcccctgt cccgaagcgc
     1141 cggagcctta ggagcctggt cgaacgctcc cgcggcccgt cgaggcgccg cgcgttgact
     1201 ccccggccgg gcgcgtctaa catccgcgca ccatgacaac ttcgcttcgc aagtcccttt
     1261 cccccgcacg tctgttcgtc gacgggcagt gggtggatcc cgtgtccggc cagtcgttca
     1321 agacgatcaa tccggcgacg gaagagccga tcaccgacgt cgccgacgcc tccgccgagg
     1381 acgtggaccg cgccgtgcag gcggcccggc gcgccttcga gtccgggccg tggtcgaaga
     1441 tgccggccgc cgatcgcggc cgcatcctgt ggaagatcgg cgatctcatc gaggccaacg
     1501 tccaggagct ggggcgcctc gagaccctcg accaggggaa gacgatcacc gagtcgaccc
     1561 gggtggacgt gccgctggcg gccgactgct tccgctactt cgccggctgg acgaccaaaa
     1621 tcgaggggga gacgatcccg gtccgcgccc cgagcctgaa ctacacgctg cgcgagccgc
     1681 tcggcgtcat cggcgccatc acgccgtgga acttcccgat cctgatggcg gcctggaaga
     1741 tcgccccggc gctcgcggcg gggaacaccg tcgtcctgaa gccggcgaaa gagacgccgc
     1801 tgaccgcgct ggtgctcgcc gagctggcga tcgaggccgg cctgccccct ggcgtcctca
     1861 acgtcgtccc gggacggggc acggtcgcgg gcgcggccct cgtcgagcac ccccacgtcg
     1921 cgaagatcgc cttcaccggg tcgaccgagg tcgggcgcga catcatgcgg cgcggctccg
     1981 actcgctcaa gcacctgacg ctcgagctgg gggggaagtc tccgaacgtg gtcttcgccg
     2041 acgccgacct cgaccaggcg gtgcgcgggg cgtcgaacgg catcttctac aacaaggggg
     2101 aggtgtgcac cgccggctcc cgcctcttcc tcgaggagag cgtgcacgac gccttcctcg
     2161 atcgcctgca gtcgcacgtc gggaagctgc agcaggggga tccactcgat ccgaagaccc
     2221 gtctcgggcc ccaggtcagc gaggcgcaga tgcgacagat cctcggctac gtggacaagg
     2281 ggaaggccga gggggcgcga ctcgtcaccg gcggcgagcg cccggccggc aaggggttct
     2341 tcgtccgccc gaccatcttc accgaggtgc gcaacgacat gacgctggcg cgcgaagaga
     2401 tcttcggacc ggtgctggcg gtgctgcgct tcaaggatct cgacgaggtg ctggcgcagg
     2461 ccaacgacag cgtctacggc ctcgccgccg cggtctggac gcgcgacatc aagcgggcgc
     2521 accagaccgc gcggcgcctc cgggccggca cggtctggat caactcctac ggcctgtacg
     2581 acaacgccat gccgttcggc ggcgtcaagc agagcggctt cggccgcgag ctcggccggc
     2641 agggactcct cgagtacacc cagacgaagt cggtctgggt cgacctctct taggcgcggc
     2701 ggccgatgcc ggagccgctg gtccgcgcgc acgccgacgc cggcgtcctg acactcaccc
     2761 tcgacgcgcc gccggccaac tgctactcgt acgacctgat gcgcgagctc gacgcctgtg
     2821 tcctcgcggc gcgcatggac ccgtcggtag acgtcatcgt cctgcgcggc gcgggggatc
     2881 gcttcttctg cgccggcgcc gacatccgga tgctcgagag cgtcacaccg gagttcaagt
     2941 acgccttctg cctgcacgcc aacgagacgc tgaggcggct cgaggagacc ccgaagctgg
     3001 tgatcgcggc tctcaacggt cacgccgtgg gcggcgggct cgaggtggcg atggcgtgcg
     3061 acctgcgcct cgcgaagaag gggggcggga agatcggact gccggaggtg tcgctcgggg
     3121 tgctgccggg gaccggcggg acccaacggc tcctgcgcct cgtcggccgg gcgaaggcga
     3181 tcgagctgat ggcgacgggc ggcaccttct cgttcgacga ggcgctcgga atgggcctcg
     3241 tgcaccgcgt cttcgaggcg gacggcttcg acgcggaggt gacgcggtac gcgcggcagt
     3301 tctgccctcc cgggaaagcg tcgctcgcgg tcgggctgat caagcgcgcg gtcgtgaccg
     3361 gcgccgagct gccgctcgcc gaggcgctcg ccctcgagcg ggagctgcag cagcgtctct
     3421 tcacctccga ggacgcgcgc gaggggctcg ccgcctacaa cgccaagcgg cccccccggt
     3481 ttcgcggccg ctgaggggcg cggcgaccct catgccggac cgcccgacgc ccccgaccga
     3541 gacgatgcgg gcggtggtcg tcccgcgcca cggcggaccg gaggtgctgg aggtcgcgga
     3601 gcgaccgcgc ccggtccccg gccccggcga ggtcctcctc caggtgaagg ccgccggcgt
     3661 caaccacctc gacaccttcg tgcggcgcgg catgccgggg atcacgctgc cgctgccgat
     3721 gatcctgggc tcggacgccg cggggaccat cgccgccgtc ggcgccgccg tcatggggat
     3781 caaggaaggg gatcggatct tcgtctcccc cggctattcg tgcgagcgct gccgcgcgtg
     3841 tctgtcgggg aacgagccgc tctgccgctt ctacggcctc ctcggcgagc accgcgacgg
     3901 cacgcaggcg gagtacgtcg cgctgcgcgc cacgcaggcg ctgccgctgc ccgacggggt
     3961 gtcgttcgag gagggggcgg cgttcccgct ggtcttcctg accgcctggc acatgctcgt
     4021 cgcgcgggcc ggcctccgtc cgggcgagga agtgctggtg cacgccggcg gcggcggcgt
     4081 cggcagcgcc gccatccaga tcgccaagct tcacggcgcc cgtgtcctga cgaccgtcgg
     4141 cggcccggag aaggcggcga aggcgagagc actcggcgcg gacgaggtga tcgatcaccg
     4201 ctccaccgat gtcctggccg aggtgcgccg cctgacctcg aagcgcggcg tggatgtcgt
     4261 cgtcgagaac gtcggccagg agacctggga gaagagtctg ctggcgctcg ccaaaggggg
     4321 acgcctcgtc acctgtggcg ccaccaccgg ctacgaggcg aagaccgacc tccggcacct
     4381 cttcttcaag gggctgtcgc tcctcggatc gacgatgggg agccgggccg agctgctgca
     4441 gatcgccgac ctcgtcgggg cgaggcgtct gcggccgatc gtggaccggg tgctgccgct
     4501 cgagcaggcg gcggaggcgc accggcttct ggcggcccgc tcggtgttcg gcaagatcgt
     4561 cctgagcccg tagaatcgcc gcgtgagctc ctcgacggtc gtcgacgcgc acctgcacat
     4621 ccagccctgg agccagttcc gccccgaggc gatccgtacc atggagaagc gcgccgacat
     4681 ggagacggtg cgcgcggtgt gcgacgatcc gaaagccctc ctgacgttcc tggaccgcga
     4741 ggggatcgat cgcgccgtcc tggtgaacta cgtcgccccg gacgtcatcg ggttcacgga
     4801 cgaggtgaac accttcatcg gccgctactg ccgcggccac gaggaccggc tggtgccggt
     4861 cggcggggtg cacccgcggc tgtcggccga tcccgccggc gacctggagc acatcgtcac
     4921 ccggcacggc gtccgggtcc tgaagctgca cccgccgcac cagctcttcc ggatgaacgc
     4981 ctatcgcgac ggcggcgacc tgccgtcgct cgcaggcctg tacgagcggg cggcggcgct
     5041 cggcgtgccg atcctggtgc acaccggcac ctccgtcttc cccggcgcgc gcaacaagta
     5101 cggcgacccg atggacctcg acgacctggc ggtggacttc ccgcgcctga cgatcatcat
     5161 ggcgcacgcc ggccggccgc tgtggatgga ggcctgcctg ttcctcctgc gccgccaccc
     5221 gaacgtgtac gtcgatctgt ccggcatccc gccgaagaat ctcctggggt acttcccgcg
     5281 cctggcggag gtcgcgcaca aggcgatgtt cggcaccgac tggcccggcc ccggcgtccc
     5341 gggcgtgcgg cagaacctcg aggcgttccg cgccctgccg ctccccgagg aagcccggcg
     5401 ggccatcctc tccggcacgg ccgatcgggt ctatcccgcg cgccgtccct aggccctcag
     5461 cggtggagca cgcggaggtc ctgttcatcg atcgcctgcc ggatcgggcg ccgccgctcc
     5521 gcggcgaggc gcgggccgat ctcgcgatcg cggggggcgg gctgaccggg ctcgcgacgg
     5581 cgctcgcggc gcgcgcgcgg ttccccaagc gccgcatcgt cctcctcgag aaggaccgct
     5641 gcggcaccgg cgccagcggg cgcaatggcg gcatggccct tcccggaacg gcgctcgatc
     5701 tcgaagacct ggaagggcgc ctcgggttcg accgggcccg gcgggtcagc gcgtggctgg
     5761 cgcgggggct cgcgatcctc atggagcggg cgcgcgcgag cggcgccgcg gcgggaccga
     5821 tcgaggaggt cggcaccctc tcgctcgcgc gcactccgcg ccatgcgcgg gcgcagagac
     5881 gccgcctcga ggtgtaccgc cggcacggcg tcgaggcgga gatcctcgag ggatcggcgc
     5941 tcgagcgggc cttccgctcg ccgctctacc gctcggcctt ccacgtgctc cggggggggg
     6001 cgctcgtcga tccgtggcga ctgacgacgg cgttgcgccg ccgcgccctc gacgcggggg
     6061 tcgtgatcca cgaagagtcg gaggtccgat cgatcgaaga gggcccggag atccgcctca
     6121 cgacccccct cggccgcctc accgcccgcg ccctcgttct cgccaccaac gccttctcgc
     6181 cgaagctggg ttttttccgc agcagggtgg cgccggtgca cgtctcctgc atcgccacgt
     6241 ccccgttgtc gcggaacgtg cgcgcggcga tcggctggga ggggcgccag gcggcgtggg
     6301 aggaggggcg cgtgtaccac ttcctgcgcc tcaccgccga cgaccgggtg cttctcggcg
     6361 gcgggaacat cgactaccgg ctcgggggac ggacccgctt cgatccctcc cgccggcacg
     6421 cgaagctgat gcggtcgctg cgcgccattt tcccgccgct caaggacgtc ccg
//
LOCUS       DASRIQ010000423         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2247633, whole genome shotgun sequence.
ACCESSION   DASRIQ010000423 DASRIQ010000000
VERSION     DASRIQ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247633"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..850)
                     /locus_tag="VFD06_10335"
     CDS             complement(113..850)
                     /locus_tag="VFD06_10335"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HZN03970.1"
                     /translation="MPIVIGQVEAIFRYPVKSMRGEPLDTATLGWHGLDGDRRFAFRR
                     LDERGGGGGFPWLTASKLPDLVLFTPRRREEGNGEAPPTHVLTPEGEEMPLFGEALAA
                     EVGRRHGAPVEMMQLKHGIFDDATISVITSDTVREVCRLAGRNADVRRFRPNIVVRST
                     RAVPFEEDEWVGGVLTFGEADGAPAVTVTMRDVRCAMVNIDPDEGSLAPEVLKAVVRA
                     NQNNAGIYGTVTRAGRLAVGQTVVLHR"
     gene            948..1643
                     /locus_tag="VFD06_10340"
     CDS             948..1643
                     /locus_tag="VFD06_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03971.1"
                     /translation="MRRSALAVLFAAGLAGLPASAQNYCAAVVPNDAVRDLKGDILSA
                     LRGVATGDRNVGNWPGSTTGMLEVGADQGTMKMIDNMWRTGLALHATLCSGANACSNA
                     RRFFQAAGTNNYLKRYGDSTSNTFQYVDLQTSYQELLRNRPFTLKGGAFEPEDWGSWY
                     AFSLGGSLWGYTKTPRGTETKTAVSGALHVSFIKVKYGSKESTNARNISDAIRGAVGD
                     KEVRLTFTEVRCN"
     gene            complement(1665..>2023)
                     /locus_tag="VFD06_10345"
     CDS             complement(1665..>2023)
                     /locus_tag="VFD06_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03972.1"
                     /translation="APRSSESSADTKVGALPEVTIKTQMPTRRDTPARKAEKKPAPAA
                     AGGEPLGVKPPRTTVPYGKAVTRQLPAMNFSTSTPAPPPAEEPVLLTEDEVEAPTGVN
                     PVVDPEEIVIDDDEPL"
BASE COUNT          310 a          711 c          688 g          314 t
ORIGIN      
        1 ggccgccctg cgtcgtcctg gaggtggagc gcggcgcggg cggcgtgcgg gcgctgaagc
       61 tgcgcagccg gctccacaag cagtggtaca cggtcccgct cccggttcgc gcctaccggt
      121 gaaggacgac ggtctggccg acggccaggc gcccggcgcg ggtgacggtg ccgtagatcc
      181 ccgcgttgtt ctggttggcg cgaaccaccg ccttcagcac ttctggagca aggctccctt
      241 cgtcaggatc gatgttcacc atcgcgcagc gcacgtcgcg catcgtgacg gtgacggcgg
      301 gcgcaccgtc cgcttcgccg aacgtgagca cgccacccac ccattcatcc tcctcgaagg
      361 ggaccgcgcg cgtggatcgg acaacgatat ttggacgaaa tcgccggacg tctgcgttcc
      421 tccccgcgag ccggcagacc tcgcgaacgg tatcggaggt gatcacggag atggtcgcat
      481 cgtcgaagat gccgtgcttc aactgcatca tctccacggg agccccgtgc cggcgcccga
      541 cctccgcggc gagggcttct ccgaacaggg gcatctcttc cccctccggc gtgaggacgt
      601 gcgtcggcgg cgcttcgccg ttcccctcct cgcgccgccg cggagtgaag aggacgaggt
      661 cggggagctt gctcgcggtc agccagggga acccgccgcc gccgccgcgc tcgtcaagcc
      721 gccgaaacgc gaagcgccga tcgccgtcga ggccgtgcca acccagcgtg gcggtatcca
      781 acggctcgcc gcgcatggat ttcaccggat aacggaaaat cgcctcgacc tgtccaatca
      841 cgatcggcat ctcgttctcc ggctcacgag gtcgtagcag ccccgcccgg gtgtgtgaag
      901 cctggttccg cctggtagaa tgccggcccc ttcctgctgg agacctcatg cgccgctccg
      961 ccctcgccgt cctgttcgcc gcgggcctcg ccggcctgcc cgcgagcgcc cagaattact
     1021 gcgccgccgt ggtgccgaac gacgcggtgc gggacctgaa gggggacatc ctcagcgccc
     1081 tgcggggcgt ggccaccggc gaccgcaacg tcggcaactg gcccggctcc accacgggga
     1141 tgctggaggt cggcgccgac cagggcacca tgaagatgat cgacaacatg tggcgcaccg
     1201 ggctggcgct gcacgccacc ctgtgctcgg gcgcgaacgc gtgcagcaat gccaggcggt
     1261 tcttccaggc ggccggcacc aacaactacc tgaagcggta cggagacagc acgagcaaca
     1321 ccttccagta cgtggacctc cagacgtcct accaggagct cctgcggaac aggccgttca
     1381 ccctcaaggg cggcgccttc gagcccgagg actggggctc ctggtacgcg ttcagcctgg
     1441 gcggcagcct ctggggctat accaagaccc ccaggggaac ggagacgaag accgccgtct
     1501 ccggcgcgct gcacgtgtcg ttcatcaagg tcaagtacgg gtcgaaggag tcgacgaatg
     1561 cccggaacat ctccgacgcc atccggggtg cggtgggcga caaggaggtc cgcctgacgt
     1621 tcaccgaggt gaggtgcaac tgacggtgca actgacgccc cccgtcacag cggctcgtcg
     1681 tcgtcgatga cgatctcctc cggatcgacg accggattga cgcccgtggg agcctcgacc
     1741 tcgtcctcgg tcagcaacac cggctcctcg gccgggggcg gcgccggcgt ggaggtggag
     1801 aagttcatcg ccggcagctg gcgggtgacg gcctttccat agggcaccgt cgtccgcggc
     1861 ggcttgacgc ccaggggctc gccaccagca gcggcggggg ccggtttctt ctcggccttc
     1921 ctcgccgggg tgtcccgccg ggtcggcatc tgggtcttga tggtcacctc ggggagcgcg
     1981 ccgaccttgg tgtccgcgct gctctcgctg gaccgcgggg ccg
//
LOCUS       DASRIQ010000424         4086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_178185, whole genome shotgun sequence.
ACCESSION   DASRIQ010000424 DASRIQ010000000
VERSION     DASRIQ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178185"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1573)
                     /locus_tag="VFD06_10350"
     CDS             complement(47..1573)
                     /locus_tag="VFD06_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HZN03973.1"
                     /translation="MTDPRPKHHHPADLSAPASPASPGSTLLRDSGIYLLGNLLQKAT
                     AFVLIPLYTTRLTTAQYGLLDLAGTVLNLGLLGAALGVPAAINKCLHRDCKDEAERGR
                     LLGTVLLFTSLSSAACALLGWAFDASLARLLFSGAEGLLVFRYTLVWLILAQLSQIPF
                     EYFRASGRSQIYVALSLSQLLAQFACTIYLVAHRGWGLEGVLAGNLAGFLLVNIVGLA
                     ALLPRVRFTVDRRLLRATITYGVAMTPVFVSGWVVNLSDRFFLQSFLGLGALGVYALG
                     YKFGALVDLLLVMPFQRAWTPIFFRFASEPEAPRTLSRIATYLGAALAFATLGISLAV
                     PPFLRVSAAPEFRAASGVVPLVGLAYLIGGLANCFGNGLIVAERVRLVAGYAAVAAVA
                     KLGLNAILIPLFGTAGAALATALAFAIQLGGILSSLGRYYPVQVEWGRLGGALAAAAV
                     PLVVSLALPEMPLVAEVAIRLALLLSYPALLILMRLPRPEEVAAATRLLSTRLIRRRI
                     "
     gene            1775..3877
                     /locus_tag="VFD06_10355"
     CDS             1775..3877
                     /locus_tag="VFD06_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03974.1"
                     /translation="MALSTRQRGIRAGLLALGFTCALAAASSAEAATYYFSNCAAGGA
                     GTQANPYCLDPAGIGKKISFQYLMDGAAPDVAAGDTIALCAGPCDGTGSATYGMQTSG
                     TTSSGGYSYVFAPRVSGTASSPITITAHTGEAVVLSGDSNQNGIPDAGEPQVMLTNVT
                     GTAANKSWYVIKNLTFEKVSQIMFYINNNPANWTFDNIEVRYSTTEMWNGGSIADAGC
                     DNEHGGYVFKLADLQGPLIIRNSRFHHICGAVHRHTVNQSLSASVLSENNEYYNVGVV
                     TNNFQGRNETWRGNYIHDFVDGITIEEDMKDIVIEDNIIACPGDYKTYSDGRCGRAIH
                     ISDGDNGPASAGKTKNITIRRNKIYGRVDGQFGGSQWGYFLCALRVTATNNTEPINVL
                     IENNMIWHHLSWSSDPICAAGIGVETNRREVTIQNNTVYDSSNGIALDATVPGIAYTV
                     RDNLLIRANKGGANKPEMVIAANASGSIVRNNNINSDGQGDPVMTVAGTNYSCSQMAS
                     YQSGNKCSATTFVRTTGVVKDWDLHLPSTDTANRSAGMAGASEDIDKTPRGSAPIDIG
                     ADEYGGSVGVTATLAVQSGGSPAPQSNGTWMLKGGSYGLTLTTSAPVVSVPGPITLTA
                     SGGGTSQWTLTGAVPGNLFTGTLTVGTSLAEGNATLTLPTGTLDDGLGNRGQLITTGS
                     AAVIDRTPPAAPTGVRTQ"
     gene            complement(3887..>4086)
                     /locus_tag="VFD06_10360"
     CDS             complement(3887..>4086)
                     /locus_tag="VFD06_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03975.1"
                     /translation="TFGAEARAALDRARVKALEIFGSGVSLEVRLVEAIPLSRSGKHL
                     YMVSLVRNPLLPRGNDTAPLL"
BASE COUNT          768 a         1398 c         1406 g          514 t
ORIGIN      
        1 cggccggccg tcgaacgcca ggaatcccgc gatgccgcac acggtttcag atcctccgcc
       61 ggatgagccg ggtggacagg aggcgggtcg ccgctgcgac ctcctccggc cgggggagcc
      121 gcatcaggat cagtagcgcc gggtacgaca ggaggagcgc caggcggatc gccacctcgg
      181 ccacgagcgg catctccggc agcgccagcg acacgacgag cggcacggcg gccgcggcga
      241 gcgccccccc cagacgaccc cattccacct gcaccggata gtaccgtccg agcgagctca
      301 ggatgccgcc gagctggatc gcgaaggcga gggcggtggc gagcgcggcg ccggcggtcc
      361 cgaacagagg gatgaggatc gcgttgaggc cgagcttggc gacggcggcg accgccgcgt
      421 agccggcgac cagtcgcacc cgctcggcca cgatgaggcc gttgccgaag cagttcgcga
      481 gaccgccgat gaggtaggcg agccccacca gcgggaccac tcccgacgcc gcgcggaact
      541 cgggcgcggc cgacacccgg aggaacggcg ggaccgccag gctgatgccg agcgtggcga
      601 aggccagggc ggcgccgaga tacgtggcga tgcgggagag ggtccgcggc gcctccggct
      661 cggaggcgaa gcggaagaag atcggcgtcc aggcgcgctg gaacggcatc accaggagga
      721 gatcgacgag cgctccgaat ttgtatccca gcgcgtagac gccgagcgct ccgagaccga
      781 ggaacgactg caggaagaac cggtcgctca ggttgacgac ccagccggag acgaacaccg
      841 gggtcatcgc gacgccatag gtgatcgtcg cgcgcagcag gcggcgatcg accgtgaacc
      901 ggacgcgcgg gagaagcgcc gcgagcccga cgatgttgac gaggaggaac ccggcgagat
      961 tcccggccag gactccttcc aggccccagc cgcggtgcgc cacgaggtag atcgtgcacg
     1021 cgaactgcgc cagaagctgc gacaggctga gcgcgacgta gatctgcgag cggcccgaag
     1081 cgcggaagta ctcgaagggg atctgcgaca gctgtgccag gatcagccag acgagggtgt
     1141 agcggaagac caggaggccc tccgccccgc tgaacagcag gcgggcgagc gaggcgtcga
     1201 aggcccagcc gagaagggcg caggcggcgg aggacaggga cgtgaagagg agcaccgttc
     1261 cgagcagccg gccgcgctcc gcctcgtcct tgcagtcccg gtgcaggcac ttgttgatgg
     1321 ccgcggggac gcccagggcc gcccccagca gccccaggtt caggaccgtc ccggcgaggt
     1381 cgagcaggcc gtattgggcg gtcgtgagcc gggtggtgta caaggggatg agcacgaagg
     1441 cggtcgcctt ctgcagcagg ttccccagga gatagatgcc cgaatcccgg agcagggtgc
     1501 tgccggggct ggcggggctg gcgggggcgc tcaagtcggc gggatgatgg tgcttcgggc
     1561 ggggatcggt caagagcgag cgattgcgat ccggtgatct gtctgacgcc ttcgtggcgc
     1621 cggtggctgc ccgaaccggt cggcgcacca aaaagggggg tgcgtccgtg gcacgttttt
     1681 cgctgacctt gggccgaata ccgggctctg gccgtgagtt gtccggcccg gcggacaagc
     1741 tctcgcaggc tcgacgcgac cgttgggggg ggacatggct ctgagcacgc gccagcgtgg
     1801 aatccgcgcc gggcttctgg ccctcggctt cacctgcgcg ctggccgccg cctcctcggc
     1861 cgaggcggcc acctactact tctcgaactg tgccgccgga ggggcgggga cccaggccaa
     1921 tccgtactgc ctcgatcccg ccgggatcgg caagaagatc tcgttccagt acctcatgga
     1981 cggggcggcc cccgacgtcg ccgcggggga caccatcgcg ctgtgcgccg gcccgtgtga
     2041 cggcaccggc agcgccacct acggaatgca gacgtccggg accacctcct cgggcggcta
     2101 ctcgtacgtg ttcgcgccgc gggtgtccgg caccgcctcc agtcccatca ccatcaccgc
     2161 ccacaccggc gaggcggtcg tcctcagtgg cgactccaac cagaacggca ttcccgacgc
     2221 cggcgaacca caggtgatgc tgaccaacgt caccggcacg gctgcgaaca agtcctggta
     2281 cgtcatcaag aacctgacct tcgagaaggt cagccagatc atgttctaca tcaacaacaa
     2341 cccggcgaac tggaccttcg acaacatcga agtccgctac agcaccaccg agatgtggaa
     2401 cggcgggagc atcgccgacg ccggatgcga caacgagcac ggcgggtacg tgttcaagct
     2461 ggcggacctg caggggccgc tgatcatcag gaacagccgg ttccatcaca tctgcggcgc
     2521 cgtgcaccgg cacaccgtca accagtccct gagcgcctcg gtcctgtcgg agaacaacga
     2581 gtactacaac gtcggggtcg tcaccaacaa cttccagggg cgcaacgaga cctggcgcgg
     2641 caactacatc catgacttcg tcgacgggat caccatcgaa gaggacatga aggacatcgt
     2701 catcgaggac aacatcatcg cctgcccggg ggactacaag acctactcgg acggacgctg
     2761 cggccgcgcc atccacatca gcgacggcga caacggtccg gcctcggcgg ggaagacgaa
     2821 gaacatcacc atccgccgca acaagatcta cggccgtgtc gacggccagt tcggcggcag
     2881 ccagtggggc tacttcctgt gcgcgctgcg ggtcaccgcc accaacaaca ccgagccgat
     2941 caacgtcctc atcgagaaca acatgatctg gcaccacctc agctggtcgt ccgacccgat
     3001 ttgcgccgcc gggatcgggg tcgagaccaa ccgcagggaa gtcaccatcc agaacaacac
     3061 ggtctacgac tcctcgaacg gcatcgcgct ggacgccacg gtgcccggga tcgcctacac
     3121 cgtgcgcgac aacctgctca tccgcgcgaa caaggggggc gcgaacaagc ctgaaatggt
     3181 gatcgccgcc aacgcgtccg gcagcatcgt ccggaacaac aacatcaact ccgacggcca
     3241 gggggatccg gtgatgaccg tcgcggggac gaactacagt tgcagccaga tggcgtcgta
     3301 ccagtcgggg aacaagtgct ccgccacgac cttcgttcgc accaccggcg tcgtcaagga
     3361 ctgggacctg cacctgccgt ccaccgacac ggccaaccgc agcgccggca tggccggggc
     3421 ctccgaggac atcgacaaga cgccgcgggg ctccgcgccg atcgacatcg gggcggacga
     3481 gtacggcggc tccgtcggcg tcaccgcgac tctcgcggtg cagtcgggcg gctcgccggc
     3541 cccgcagagc aacggcacct ggatgctcaa gggcggaagc tatggtctga cgctgaccac
     3601 gagcgctccg gtggtttcgg tgccggggcc catcaccctg accgccagcg gcggcggcac
     3661 gtcgcagtgg acgctgaccg gggcggtccc ggggaacctc ttcaccggca cgttgaccgt
     3721 cgggacgagc ctcgccgaag ggaacgccac gttgacgctt ccgaccggaa ccctcgacga
     3781 cggcctgggg aaccgcgggc agctgatcac caccgggtcg gccgccgtga tcgatcgcac
     3841 cccgcccgcc gcgccgaccg gcgtccggac gcagtagccg ctcccgtcag aggagcggcg
     3901 ccgtatcgtt cccccgcgga agcagcggat tgcgcaccag cgacaccatg tagaggtgct
     3961 tgcctgagcg cgacagcggg atcgcctcca ccaggcggac ctcgagactc actccggacc
     4021 cgaagatctc cagcgccttg acgcgcgccc ggtcgagcgc cgcgcgcgcc tccgctccga
     4081 acgtcg
//
LOCUS       DASRIQ010000425         7504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1991985, whole genome shotgun sequence.
ACCESSION   DASRIQ010000425 DASRIQ010000000
VERSION     DASRIQ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991985"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFD06_10365"
     CDS             <1..783
                     /locus_tag="VFD06_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZN03976.1"
                     /translation="LSQSGQLYLEPACMAFGKVYCFGPTFRSEKSKTRRHLAEFWMVE
                     PEVAYLELPGLLELAEDFVSTLVTRVLERCGPELKTLERDTAKLEKVQKPFQRLTYDE
                     AAAILLKPENQARMREHDSPPFVAGNDFGGFDETLLTEGLDRPVMVTHWPADIKAFYM
                     QPDAKDPSRALCVDVLAPEGYGEIIGGSQRIHDHDLLLKRIQEHHLPVEAFQWYLDVR
                     RYGSVPHAGFGMGIERFVSWVAGIHHLREAIPYPRTLNRLYP"
     gene            780..2144
                     /gene="rimO"
                     /locus_tag="VFD06_10370"
     CDS             780..2144
                     /gene="rimO"
                     /locus_tag="VFD06_10370"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZN03977.1"
                     /translation="MIKVGLVSLGCPKNLVDSEVMLGTLEGKGYELTPDPADADVIVV
                     NTCTFIEPARKESIDTILEMAELKKHGRCRRLVVAGCMVQAHHDTLKKEIPEIDALVG
                     LNDIERIAEACALEAGRKFEASQEAAHYLYTERSPRRLATPGYSAYMKISEGCDHTCA
                     FCAIPSFRGTQRSRPITSLVEEARRLASAGTVELNLIAQDTTDYGTDLGDGTTAAGLL
                     RALDEVDGLRWIRVLYAYPNRITTDFIAALAGGRRIARYLDLPLQHADASLLKAMRRG
                     GDGDAHLRLLERLRQAIPEVALRTTFIVGFPGETEAQFQTLLEFVRAARFDRVGAFTY
                     SEEKGTHAAALPDDVPPGVKEERRARLMEAQAGVAEERHRRLVGRTIEVLVEGPHEES
                     DLLLSGRSEGQAPEIDGTVILTDAPRVLYPGEFVEVRIDEAHPHDLVGAVVGVPARPA
                     AARP"
     gene            2144..2635
                     /locus_tag="VFD06_10375"
     CDS             2144..2635
                     /locus_tag="VFD06_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZN03978.1"
                     /translation="MNGPRPPLDAIGLGVATLLGVGHSPIIPGTAGTLASLPLALVAA
                     AFLPLPAYAALTLGVTLLGIWASGRASAALGLKDPGVVVIDETAGLFVTFLGISPGWT
                     AAVLGFFCFRIMDIVKPPPARQFERLPGGFGIMLDDLMAGVYANLLVRAGLFVIGFVT
                     TVR"
     gene            2632..3567
                     /locus_tag="VFD06_10380"
     CDS             2632..3567
                     /locus_tag="VFD06_10380"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZN03979.1"
                     /translation="MIVETRPEVLRPRLSNPVATIGNFDGVHRGHQAIFARVLERAGA
                     LGGTSVAITFDPHPLKVLAPGRAPDLIATPRQKLAFIEAAGIDAVLNLRFDAALADTG
                     AEEFVDAYLRDALALREVYVGATFNFGRGREGTADTLVALCAARGITAGKVGEVRFLG
                     SPVSSSRIRRAVRAGEVELAAELLGRPFAVEGTVRHGAGRGAGLGFPTANLEARSELV
                     PGDGVYVTRTRAGDSVLSSVTNIGSRPTFADQDFAVETHLLDAQLDLYDSEIEVSFLA
                     RLRQELRFASAQALVEQVHRDVERARAWFAAEAGR"
     gene            3624..4292
                     /gene="tmk"
                     /locus_tag="VFD06_10385"
     CDS             3624..4292
                     /gene="tmk"
                     /locus_tag="VFD06_10385"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZN03980.1"
                     /translation="MPSRPGGVVLSGFITFEGIEGSGKSSQIALLSEALRARGLAHVL
                     TREPGGTPAGESIRGLLLDSATRLGPPAELLLYAAARAEHLERIIRPALAIGRLVLCD
                     RYLDATRAYQGYGRGLPLAWIESLHALEPLRLAPDRTVLLDLDPAVGLERARGRNRKA
                     ARKAAPDEGRFEAEDLAFHRRVRDGYLSLAKAEPKRFHIVDGDAPEDVVQKRIQEALC
                     ISAE"
     gene            4274..5353
                     /locus_tag="VFD06_10390"
     CDS             4274..5353
                     /locus_tag="VFD06_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN03981.1"
                     /translation="MHLRGVIGQPRAVEALERSSASGRFHPSLIFHGPPGVGKLTAAL
                     ALARALLCTGEDPPCGACHACRRIEARSQRHPDLFVAFPERVDDFRKGETAEGVSGID
                     LQEKQDAAAASAALSLLVDRLREAIAFVHRRPVEGRRSILIIDQAQRLAPEGANALLK
                     TIEEPPAHAVIALLATSLHALLPTIRSRCQAVPFQSLSPGAIASWLISERGLPEGEAR
                     LRAALSGGRIGTAMGLDLEAFRDRRDRLLAALESFLRPPDPGRALARAEEIAKGESPE
                     QDLEILTSLLRDRMIVSQGSGQPAGLIHADVARRLRDLPLQGGRDGTEALLALEGALE
                     GLRRRGNRQLLIENAFLGFLPRPVS"
     gene            5365..7098
                     /locus_tag="VFD06_10395"
     CDS             5365..7098
                     /locus_tag="VFD06_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN03982.1"
                     /translation="MSFFRGALRAYRREFLLGLLCLVVTNGLMLVIPWLLKHGIEALQ
                     ARAPLSLVGRHALAIAVVALLLAGIRTWSRLHVLGASRRIVADLRARVFTHLQTLPAS
                     FYARHRTGEIMSRAVNDLLLIRSLFGPGILNLINVSILYTAGLAMMAVLDPVLTLAAV
                     LPYPFLLVAVSRVSRTIHHRSNAAQEQLAEISNKAQENLSGIHQVKAYAREDLEVEAF
                     AGLSSEYRQRNLALARSRGLIVVLMTGLGGLSTIVVLWLGGRHVIAGTLSLGGLVAFT
                     AYLAILTGPTIMMGWVLGVFQRGLGAIRRVEEILSQTSDLPGDLLAGPCPPLSGAISF
                     RRLSFAYPGEGREVLHDIDLDVPAGSIAGIVGPVGSGKSTLVRLLGAVFPVGRGRLLL
                     DGHDFNDLPTRGLREQVATVPQETFLFSRTIAENIALGRPEATRAEIEAAAQIAQLTR
                     DLPSWPHGLDTRVGERGVTLSGGQRQRVAIARAVLMDPRILVLDDALSSVDADTEAAI
                     LAGLRRFMRGRTTLLISHRVATVAGADRLLVLDEGRVVETGSPSELLARDGPFARLSR
                     AQQIERELEAM"
     gene            7095..>7504
                     /locus_tag="VFD06_10400"
     CDS             7095..>7504
                     /locus_tag="VFD06_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZN03983.1"
                     /translation="MSRAAAFGGKTRATTPPPDDDVVGRVYDARLVRRLLAFVRPEAA
                     LIALSVAFMFAVMGASLVQPWLLMRLIDEHIVPGRWQGVTALALLYLGAFVLELLARF
                     GQLYTMERTGQNVIQALRVKVFARMQRLDMAFFDR"
BASE COUNT         1032 a         2765 c         2563 g         1144 t
ORIGIN      
        1 ctcagccagt cggggcagct ctacctggag ccggcgtgca tggccttcgg caaggtgtac
       61 tgcttcggac cgaccttccg ctccgagaag tccaagaccc ggcgccacct cgccgagttc
      121 tggatggtgg agccggaggt cgcctacctc gagctgccgg gcctgctcga gctcgcggag
      181 gacttcgtct cgaccctcgt gacccgcgtg ctcgagcgct gcgggccgga gctgaaaacc
      241 ctcgagcgcg acaccgcgaa gctggagaag gtgcagaagc ccttccagcg cctcacctac
      301 gacgaggcgg cggccattct cctgaagccg gagaaccagg cgcgcatgcg cgagcacgac
      361 tcgccccctt tcgtcgccgg gaacgacttc ggaggcttcg acgaaacgct tctgaccgag
      421 gggctcgacc ggccggtgat ggtcacgcac tggccggccg acatcaaggc gttctatatg
      481 cagcctgacg cgaaggaccc gtcgcgcgcc ctgtgcgtgg atgtgctcgc tcccgaaggc
      541 tacggcgaga tcatcggtgg tagccagcgc atccacgatc acgacctgct cctgaagcgc
      601 atccaggagc accacctgcc ggtcgaggcg ttccagtggt acctcgacgt gcggcgctac
      661 ggcagcgtgc cacacgccgg cttcggcatg gggatcgagc gcttcgtgtc gtgggtcgcc
      721 ggcatccacc atctgcgcga ggcgatcccg tacccgcgaa cactgaaccg cctctacccg
      781 tgatcaaggt cggactggtc agtctcggct gtcccaagaa cctcgtggac agcgaagtga
      841 tgctcggaac cctcgagggg aaggggtatg agctcacgcc cgacccggcg gacgccgacg
      901 tcatcgtcgt caacacctgc accttcatcg agccggcccg caaagagtcg atcgacacca
      961 tcctcgagat ggccgagctc aagaaacacg gccgctgccg ccggctcgtg gtcgccggct
     1021 gcatggtgca ggcgcaccac gacacgctga agaaagagat cccggagatc gacgcgctcg
     1081 tcggcctcaa cgacatcgag cggatcgccg aggcgtgcgc cctcgaagcg ggccggaagt
     1141 tcgaggccag ccaggaggca gcgcactatc tctacaccga gcgctcgcca cgccggctcg
     1201 ccacccccgg ctacagcgcc tacatgaaga tctccgaagg gtgcgatcac acgtgcgcct
     1261 tctgcgccat cccctcgttc cgggggacgc agcgctcgcg gcccatcaca tcgctcgtcg
     1321 aggaggcgcg gcggctggcc tccgccggca cggtcgaatt gaacctgatc gcccaggaca
     1381 cgaccgacta cggcaccgac ctcggggacg gcacgacggc ggccggtctc ttgcgcgcgc
     1441 tcgacgaggt ggacgggctg cgctggatcc gggtgctcta cgcctatccc aaccggatca
     1501 ccacggactt catcgccgcg ctcgcaggcg ggcggcgcat cgcacgctac ctcgacctgc
     1561 cgctgcagca cgccgacgcg tcgctcctga aggcgatgcg gcggggcggc gacggcgacg
     1621 cgcacctgcg cctcctcgag cggctgcggc aggcgatccc ggaggtcgcg ctccgcacca
     1681 cgttcatcgt cggcttcccg ggagagaccg aggcgcagtt ccagaccctc ctcgagttcg
     1741 tccgggcggc gcgcttcgat cgggtcggcg cgttcaccta ctcggaggag aaagggacgc
     1801 acgccgcggc gcttccggac gacgtcccgc ccggggtcaa ggaggagcgc cgcgcgcggc
     1861 tgatggaggc ccaggccggg gtcgccgagg agcgtcaccg gcgcctcgtc ggcaggacga
     1921 tcgaagtcct ggtcgaaggg ccgcacgaag agagcgatct gctcctgagc gggcggagcg
     1981 aagggcaggc gccggagatc gacgggacgg tcatcctcac cgacgcgccg cgcgtcctct
     2041 acccgggcga attcgtcgag gtccggatcg acgaggcgca tccgcacgat ctcgtcggcg
     2101 ccgtcgtcgg tgtccccgcc cgtccggccg ccgcgcgccc ctgatgaacg gcccccgccc
     2161 gccgctcgat gccatcggcc tcggcgtcgc cacgctcctc ggcgtcggtc attcgccgat
     2221 catcccaggc accgccggga cgctcgcgtc gctgccgctc gccctcgtcg cggccgcgtt
     2281 cctgccgctg ccggcgtacg cggcgctcac cctgggcgtg acgctgctcg ggatctgggc
     2341 gtcgggccgc gcctccgcgg cgctcgggct caaggatccc ggcgtcgtcg tcatcgacga
     2401 aacggcgggg ctgttcgtga cgttcctcgg catctccccc ggctggacgg cggcggtgct
     2461 cgggttcttc tgcttccgga tcatggacat cgtgaaaccg cctccggccc gccagttcga
     2521 gcgcctcccc ggaggcttcg ggatcatgct cgacgacctc atggccggcg tctacgccaa
     2581 cctcctcgtg cgcgccggcc tgttcgtcat cggcttcgtg acgacggtgc gatgatcgtc
     2641 gagacgaggc cggaagtgct gcgcccgcgt ctcagcaacc cggtcgcgac gatcgggaac
     2701 ttcgacggcg tgcaccgcgg gcaccaggcg atcttcgccc gcgtcctcga acgggccgga
     2761 gcgctcggcg gcacgagcgt ggcgatcacc ttcgatccgc acccgctcaa ggtgctggcc
     2821 cccggccggg cccccgatct catcgccacg ccgcggcaga aactggcgtt catcgaggcg
     2881 gccggcatcg acgcggtcct gaacctccgc ttcgacgcgg ccctcgccga caccggggcg
     2941 gaggagttcg tggacgcgta cctgcgcgac gcgctggcgc tgcgcgaggt gtacgtcggc
     3001 gcgaccttca acttcggccg cgggcgggaa gggaccgccg acacgctcgt cgccctgtgc
     3061 gcggcgcgcg gcatcacggc cgggaaggtt ggagaagttc gtttcctcgg gagccccgtg
     3121 agcagcagcc gcatccggcg cgccgtccgc gcgggagagg tcgagctggc ggcggagctc
     3181 ctcggccgcc cgttcgcggt ggaggggacg gtgcgacacg gcgccgggcg cggcgccgga
     3241 ctcggcttcc cgaccgcgaa tctcgaagcc cgcagcgagc tcgtcccagg ggacggggtc
     3301 tacgtcacgc gcacgcgggc cggcgacagc gtcctctcgt cggtgaccaa catcggatcg
     3361 cgtccgacgt tcgctgacca ggacttcgcg gtcgagacgc acctgctcga cgcgcaattg
     3421 gatctctacg acagcgagat cgaggtgtcg ttcctggcgc gcctgcgcca ggaattgcgc
     3481 ttcgcctccg cccaggcgct cgtcgagcag gtgcaccgcg acgtcgagcg ggcgcgcgcc
     3541 tggttcgccg ccgaagcggg ccgctgaacc gcgtgtccgg acgggcctcc cgcctccgga
     3601 aggggccgga tcggcgtgct accatgccct ctcggccggg aggggtggtg ctcagcggct
     3661 tcatcacgtt cgaagggatc gaaggctccg ggaaaagctc ccagatcgca ctcctgtccg
     3721 aggcgctgcg cgcccgcggc ctcgcccacg tcctgacgcg cgagcccggt gggacgccgg
     3781 cgggagagag catccgcggc ctcctgctcg attccgcgac gcggctcggc ccgccggccg
     3841 agctcctgct ctacgccgcg gcccgcgccg agcacctcga acggatcatc cgcccggcgc
     3901 tcgccatcgg gcgcctggtg ctgtgcgatc gctatctcga cgccacgcgc gcctaccagg
     3961 ggtacggccg cggcctgccg ctcgcgtgga tcgagtcgct gcacgcgctc gagccgctgc
     4021 ggctcgcccc cgatcgcacc gtccttctcg atctcgaccc cgccgtcggg ctcgagcggg
     4081 cgcgcggccg caaccgcaag gccgccagga aagccgctcc ggacgagggg cgtttcgagg
     4141 cggaggatct ggccttccat cggcgcgtgc gggacggtta tctgtcgctg gcgaaagcgg
     4201 agccgaagcg cttccacatc gtcgacgggg acgcgcccga agacgtcgtg cagaaaagaa
     4261 tccaggaggc gctgtgcatc tccgcggagt gatcgggcag ccgcgcgccg tcgaggcgct
     4321 ggagcgctcc tccgcatcgg gtcgcttcca cccctcgctc atcttccacg gcccgcccgg
     4381 ggtcgggaag ctgaccgccg ccctggccct cgcccgggcg cttctctgca ccggggagga
     4441 tcctccgtgc ggcgcgtgcc acgcctgccg ccgcatcgag gcgcggtcgc agcgccatcc
     4501 cgacctcttc gtcgccttcc cggagcgcgt ggacgatttc cgcaaggggg agacagccga
     4561 gggggtctcc gggatcgacc tgcaggagaa acaggacgcg gccgccgcga gcgccgccct
     4621 gtcgctcctc gtcgatcgcc tgcgcgaggc gatcgccttc gtgcatcgcc ggccggtcga
     4681 ggggcggcga tcgatcctca tcatcgacca ggcccagcgc ctcgccccgg aaggggcgaa
     4741 cgcgctcctc aagacgatcg aggaaccgcc ggcgcacgcg gtgatcgcgc tcctcgccac
     4801 gtcgctgcac gcgctcctgc cgaccatccg ttcgcgctgc caggcggtgc cgttccagtc
     4861 cctctccccc ggcgccatcg cctcgtggct gatctccgag cgcgggcttc cggagggcga
     4921 ggcgcgtctc cgcgccgcgc tttccggcgg ccggatcggc accgccatgg ggctggatct
     4981 cgaggccttc cgggaccggc gcgatcgact cctcgcggcg ctcgaatcgt tcctccgccc
     5041 tcccgacccc ggccgggcgc tggcgcgcgc cgaggagatc gccaaggggg agtctcccga
     5101 gcaggacctc gagatcctca cgagcctgct gcgcgaccgg atgatcgtct cccagggatc
     5161 cggccagccg gccggtctca tccatgccga cgtggcgcgc cggctgcgcg atctgccgct
     5221 ccagggcggt cgggacggca ccgaggcgct cctcgcgctc gaaggggcgc tcgaaggcct
     5281 ccggcgccgc ggcaaccggc agctcctgat cgagaacgcc ttcctggggt tcctgccgcg
     5341 gccggtgtcc tgagggccgg atcgatgagt tttttccgag gcgcgctgcg cgcctaccgg
     5401 cgcgagttcc tgctcggtct tctctgcctc gtcgtcacga acggactgat gctcgtgatc
     5461 ccgtggctcc tgaagcacgg catcgaggcg ctgcaggcgc gggcaccgct gtcgctcgtc
     5521 gggcgccacg cgctcgccat cgccgtcgtg gcgctcctgc tcgcggggat tcgcacctgg
     5581 tcgcggcttc acgtcctcgg cgcatcgcgc cgcatcgtcg ccgacctgcg cgcccgcgtc
     5641 ttcacccatc tgcagaccct gccggcgtcg ttctacgcgc gccaccgcac gggcgagatc
     5701 atgtcgcgcg ccgtcaacga tctcctcctc atccggtcgc tgttcggtcc cggcatcctg
     5761 aacctcatca acgtgtcgat cctgtacacc gccggactgg cgatgatggc ggtgctcgat
     5821 ccggtcctga ctctcgccgc cgtcctgccg tacccctttc tcctggttgc ggtgagccgc
     5881 gtcagccgca ccatccacca ccgcagcaac gccgcccagg agcagctcgc ggagatcagc
     5941 aacaaggcgc aggagaacct gagcggcatc caccaggtga aggcctacgc gcgcgaggac
     6001 ctcgaggtgg aggcgttcgc gggcttgtca tcggagtacc gacagcgcaa cctggcgctg
     6061 gcgcgcagcc gcggcctcat cgtcgtcctg atgaccgggc tcggcgggct cagcaccatc
     6121 gtcgtcctgt ggctcggcgg ccggcacgtc atcgccggga cgctcagcct cggcggcctc
     6181 gtcgccttca cggcgtacct ggcgatcctg accggcccga ccatcatgat gggctgggta
     6241 ctcggggtgt tccagcgggg attgggggcg atccgccgcg tggaggagat cctctcccag
     6301 acgagcgacc tcccgggcga cctcctggcc ggcccgtgcc cgcccctgtc cggggcgatc
     6361 tccttccgcc gcctgtcgtt cgcgtatccc ggcgaaggac gcgaggtgct gcatgacatc
     6421 gacctcgacg ttcccgccgg atcgatcgcc ggcatcgtgg gaccggtcgg ctccgggaaa
     6481 tcgaccctgg tgcgactgct cggggcggtg ttcccggtcg gacgcgggag gctcctcctc
     6541 gatggacacg acttcaacga cctgccgacc cgtggcctgc gcgagcaggt cgccaccgtc
     6601 cctcaggaaa cctttctctt ctcgcgcacc atcgccgaga acatcgccct cggccgaccg
     6661 gaggccacgc gggccgagat cgaggcggcg gcgcagatcg cgcagctgac gcgcgatctc
     6721 ccctcctggc cgcacgggct cgacacccgc gtgggcgagc gcggcgtcac gctgtcgggc
     6781 ggtcagcgcc agcgggtggc catcgcccgc gccgtcctga tggatccgcg catcctggtc
     6841 ctcgacgacg ccctttcgag cgtggacgcc gataccgaag cggcgatcct cgcggggctg
     6901 cgccgcttca tgcgcgggcg caccaccctc ctgatctcgc accgggtcgc cacggtggcg
     6961 ggggccgatc gcctgctcgt cctcgacgaa gggcgggtgg tcgagaccgg gtcgccgtcc
     7021 gagctgctcg cacgggacgg ccccttcgca cgcctgtcgc gcgctcagca gatcgagcgc
     7081 gagctggagg cgatgtgagt cgcgccgccg ccttcggcgg aaaaacccgc gcgacgacgc
     7141 cgccccccga tgacgatgtc gtgggccggg tgtacgacgc gcggctggtc aggcgactgc
     7201 tggccttcgt gcggccggag gcggccctca tcgcgctgtc ggtcgccttc atgttcgcgg
     7261 tgatgggcgc gagcctggtg cagccgtggc tcctgatgcg actcatcgac gagcacatcg
     7321 ttccgggtcg ctggcagggg gtcaccgccc tggcgcttct gtacctcggc gcgttcgtcc
     7381 tcgagctcct ggcgcgcttc ggccagctct acacgatgga gcggaccggg cagaacgtca
     7441 tccaggcgct ccgcgtcaag gtcttcgcgc gcatgcagcg gctcgacatg gcgttcttcg
     7501 atcg
//
LOCUS       DASRIQ010000426         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2499905, whole genome shotgun sequence.
ACCESSION   DASRIQ010000426 DASRIQ010000000
VERSION     DASRIQ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499905"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..2377
                     /locus_tag="VFD06_10405"
     CDS             176..2377
                     /locus_tag="VFD06_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZN03984.1"
                     /translation="MTERTALLLIVPAVLFSLQGCASSGGADPVTTVSAPRPAAPPAR
                     NIGKPNEVSSKSDEAEKNAEPAEDPELPKHDAPGEPSGTRETTPDRTQRDQLPETVQR
                     VQDGMEEAYQAGLEAYQAGRFVEAKEGFDRAVDMVLSSGIDLDQYPGLRAAFDTMVAD
                     ISDMDTDLYRQDAPPDGGENASPLDSLKDITTFLSPEEAEKERQKIQKVVGAISYDIP
                     ITLNPKVLGFIEAFQTRIRREFEAGLQRSGMYLPLIKSIFREEGLPEDLAYMAHQESA
                     FKNTAYSRARAKGMWQFMSFTGRRYGLRIDPWVDERSDFEKATRAAARYLKDLHERYD
                     DWYLAMAAYNAGEGKIDRAIARARTRDFWAISKTRYIRAETKSYVPAILASILIDKSP
                     EDYGFKVETNPPLRWERVEIDQPTDLQVLADGCAAQLEAIRFLNPELRGLVTPPNVGR
                     YSARVPVGVKDALLAHLTAIPQEKRVSWTVHEVKNGETFTTVARRYKVPVRALVDANP
                     RYAGKRLRGGWTLNVPLVAGAPVQTAAATDRPTFEAGERVVHRVRKGENLNAVAGKYR
                     TTVANLKRWNRLGSTVIRPGQRLVAYYGEKGDGPHLEVDPSTPVTIASGRIEYRVQQG
                     DTLNSISRKFSAPLDDLLRWNTLTTGSVIRPGDRLWVGEPPAGAVPQGATEAGATASA
                     AEGTTITYRVRKGDTLHRIARLYDVTVGQVRSWNQLESTVLVPGQVLKIER"
     gene            2446..>3313
                     /locus_tag="VFD06_10410"
     CDS             2446..>3313
                     /locus_tag="VFD06_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN03985.1"
                     /translation="MSPKRPRPGLWVLAALFLMVACAGGTSGNREKASSAEELEGALA
                     HGDAAFKDGDYAEALKAYEAVRRIQPDHPRAVVAGATCLLKTHHVKTAKDLLADYLSR
                     HADDVAARLTLARTLLRGAEFDAAAVELRRVRQADPQNLLALYNLGFIAYRNGDYAEA
                     EKDLKEALRVRPEHPEAHYTLGLTYLALGRNDEAVAELKEAVRLDPSHVGAHFNLAKA
                     AALAGQNDLAASERQAFATLSGKTKAETERSEQVKSQSLKAVQFLMAENFPEALREYQ
                     ALLARYPDHAPLY"
BASE COUNT          565 a         1143 c         1148 g          457 t
ORIGIN      
        1 gggaacgcaa gggtcgggcc atgcgttgta ccgtccgtcg gcgcccgttt gaaacggcct
       61 cgaaatcgtg atcgtgaatt tccggccggt ttcttgacgg agttccgcgg ccccgatata
      121 tttctcgggc gcgccgcgtc cgcggatgga cgccgcgaac cgttcaagga tgagagtgac
      181 cgaacgcacg gcgctactcc tcattgttcc tgcagtgctc ttctccctcc aggggtgtgc
      241 ctccagcggc ggcgccgacc cggtgacgac cgtctccgcg ccgcgccccg cggcaccgcc
      301 cgcccgaaac atcgggaagc ccaacgaggt ctcgagcaag tcggacgagg cggagaagaa
      361 tgccgagccg gccgaggacc cggaactgcc gaagcacgat gcgccgggtg agccgtcggg
      421 gacgcgcgag accaccccgg atcgcaccca gcgggatcaa ctccccgaga cggtgcagcg
      481 ggtgcaggac ggcatggagg aggcgtacca ggccggcctc gaggcgtacc aggcaggccg
      541 cttcgtcgag gcgaaggaag gcttcgaccg cgccgtcgac atggtcctct cgagcggcat
      601 cgatctcgat cagtacccgg ggctgcgcgc agcgttcgac accatggtcg ccgacatcag
      661 cgacatggac acggatctct accggcagga cgctccgccc gacggaggcg agaacgcctc
      721 tcccctcgac tccctcaagg acatcaccac ctttctcagc ccggaggagg cggagaagga
      781 gcgacagaag atccagaagg tggtcggggc gatctcctac gacatcccga tcacgctgaa
      841 cccgaaggtc ctcgggttca tcgaggcctt ccagacccgc atccgccggg agttcgaggc
      901 cgggctgcag cgctccggca tgtacctgcc gctcatcaag tcgatcttcc gcgaggaggg
      961 gctgccggag gacctggcct acatggcgca ccaggagtcg gcgttcaaga acaccgccta
     1021 ctcccgcgcc cgcgccaagg ggatgtggca gttcatgtcg ttcacgggcc gcaggtacgg
     1081 cctgcggatc gatccctggg tggacgagcg ctccgacttc gagaaggcga cccgcgcggc
     1141 ggcgcgctac ctcaaggacc tgcacgagcg ctacgacgac tggtacctgg cgatggcggc
     1201 ttacaacgcg ggcgaaggga agatcgaccg cgccatcgcg cgggcccgca cccgcgactt
     1261 ctgggccatc tccaagaccc gctacatccg cgccgagacc aagtcgtacg tgccggcgat
     1321 cctggcctcc atcctcatcg acaagtcgcc cgaggactac ggcttcaagg tggagaccaa
     1381 cccgccgctg cggtgggagc gggtggagat cgaccagccg accgacctgc aggtcctcgc
     1441 ggacgggtgc gcggcgcagc tcgaggcgat ccgcttcctg aacccggagc tgcggggcct
     1501 cgtgacgccg cccaacgtgg gccggtactc cgcccgcgtg ccggtggggg tgaaggatgc
     1561 gcttctggcg cacctgaccg cgatcccgca ggagaagcgg gtgtcgtgga ccgtgcacga
     1621 ggtgaagaac ggcgagacct tcacgaccgt cgcgcggcgg tacaaggtcc cggtgcgggc
     1681 gctggtggac gccaacccgc gctatgccgg caagcgcctg cggggcggct ggacgctcaa
     1741 cgtgccgctc gtcgccgggg cgccggtgca gacggccgcc gccaccgatc gcccgacctt
     1801 cgaggcgggg gaacgggtgg tgcaccgggt tcgcaaaggg gagaatctca atgcggtggc
     1861 cgggaagtac cgcacgactg tcgccaacct gaagcgctgg aaccggctgg ggagcacggt
     1921 catccgtccc gggcagagac tggtggcgta ctacggggag aagggagacg gtccgcatct
     1981 cgaggtggat ccctccaccc cggtcaccat cgcctccggc cggatcgagt accgggtgca
     2041 gcagggggac acgctcaact ccatctcgcg caagttcagc gcgccgctcg acgacctgct
     2101 ccgctggaac accctgacga ccggaagcgt catccgccct ggggatcgcc tctgggtcgg
     2161 cgagccgccg gcgggggcgg tgccgcaggg tgcgacggag gcgggtgcca ccgcctcagc
     2221 cgccgagggg acgaccatca cgtacagggt gcgcaaggga gacacgctgc accgcatcgc
     2281 ccgcctgtac gacgtgacgg tcgggcaggt ccgctcctgg aaccagctcg agtcgaccgt
     2341 cctggtcccc ggccaggtcc tgaagatcga gcggtagctc cggggggctt cgcctcctcc
     2401 cgccctgcgt ttcgattcgt accctcctgc taagatgacc ggctcatgag cccgaaacgt
     2461 cccaggccag gcctctgggt gctcgcggcg ctgttcctga tggtcgcgtg cgccggaggg
     2521 acgtccggca accgcgagaa ggccagctca gccgaggagc tggagggggc cctggcgcac
     2581 ggcgatgcgg ccttcaagga cggcgactac gcggaggcgc tcaaggcgta cgaggcggtg
     2641 cggcgcatcc agcccgatca cccccgggcc gtggtggcgg gggcgacctg cctcctcaag
     2701 acgcaccatg tgaagacggc gaaagacctg ctcgccgatt acctgagccg ccacgcggac
     2761 gacgtcgcgg ctcgcctgac cctggcgcgc acgctcctgc gcggcgcgga gttcgacgcc
     2821 gccgcggtgg agctgcgccg ggtgcgccag gcggatccgc agaacctcct ggcgctctac
     2881 aacctcggct tcatcgccta ccggaacggc gactacgccg aggccgagaa ggatctcaag
     2941 gaggcgctgc gcgtgcggcc ggagcatccc gaggcgcact acacgctcgg cctgacctac
     3001 ctggcgctcg gccgcaacga cgaggcggtc gccgagctga aggaagcggt ccgcctcgat
     3061 ccgtcgcacg tcggggctca tttcaatctg gcgaaagcgg cggcgctcgc cggccagaac
     3121 gacctggccg cctccgagcg gcaggccttc gcgaccttga gcgggaagac caaggcggag
     3181 accgagcgct ccgagcaggt caagtcgcag agcctcaagg cggtgcagtt cctgatggcg
     3241 gagaacttcc ccgaggcgct ccgggagtac caggcgcttc tcgcccgcta tcccgaccac
     3301 gcgcccctct aca
//
LOCUS       DASRIQ010000427         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1227013, whole genome shotgun sequence.
ACCESSION   DASRIQ010000427 DASRIQ010000000
VERSION     DASRIQ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227013"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..712)
                     /locus_tag="VFD06_10415"
     CDS             complement(8..712)
                     /locus_tag="VFD06_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN03986.1"
                     /translation="MEPSPHANASLTDEEIVRRVRGGEPALFEVLMRRYNQRLYRVAR
                     AILGSDAEVEDVMQEAYLRAYAHLGQFAGRSRFSTWLTRIAAHAAAARGRQTTCDLFA
                     AHDGVAAAALSGASGGGSRPPREDADAGAIDPERRLLQGEVRALLEQALDRLTPDTRS
                     VFVLREVEGLNTAETAEALGVSEEAVRTRLLRARAMLRRDLSRAGIETAAAYRLHLER
                     CDRVVGGVLPHLIPAA"
     gene            859..2196
                     /locus_tag="VFD06_10420"
     CDS             859..2196
                     /locus_tag="VFD06_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012484724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN03987.1"
                     /translation="MRISSKWITAGAALMLSLAFAGPGGNAGDRKDARRPGVEGPVAD
                     NAVSMLAEGRRTFRFDTFGDEEFWGDTLRLHQAIAGSALGGVGPGVSPRTALVVGLKV
                     DSEALPSSLAESLRRGQVNLDSPATTVALLKLHAVVGVTGFFDDGGRLVSMGIQCALC
                     HSTVDNSVAPGIGRRLDGWANRDLDVGTIIALAPDLQVLADLLGVSQDTVRTVLRSWG
                     PGKFDAELILDGKAFRPDGKPAATLLPPAFGLAGVNLHTWTGWGSVTHWNAFVANLEM
                     HGKGTFYDPRLNDPVKFPVAARAGFSDVRSDEDRITPRLAALHFYQLALPAPPAPAGS
                     FDRAAADRGRNVFLGRGQCATCHVPPLFTEPGWNMHTPEEMHIDDFQALRSPDERYRT
                     SPLRGLWTHTKGGFYHDGRFANLEAVVSHYNTTRGLTLSEQERRDLVEYLKSL"
     gene            complement(2350..2700)
                     /locus_tag="VFD06_10425"
                     /pseudo
     CDS             complement(2350..2700)
                     /locus_tag="VFD06_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
BASE COUNT          375 a          959 c          953 g          420 t
ORIGIN      
        1 caggctccta ggcggcgggg atgaggtgtg gcaggacgcc gccgacgacc cggtcgcagc
       61 gctcgaggtg cagccggtag gccgcggcgg tttcgattcc agcacgggac agatcgcggc
      121 gcagcatggc ccgcgccctc agcaggcgcg tccgcacggc ctcctcgctg acccccagcg
      181 cctcggcggt ctccgcagtg ttcagcccct cgacctcccg cagcacgaag accgaacggg
      241 tgtcgggagt gagccggtcg agcgcctgct cgaggagcgc gcgcacctcc ccctgcagga
      301 gccggcgctc ggggtcgatg gcgccggcgt ccgcatcctc ccgaggcggc cgggagccac
      361 ccccggaggc gccggacagc gccgccgcgg cgacgccgtc gtgcgccgcg aacaggtcgc
      421 aggtggtctg gcggccgcgg gccgccgccg cgtgcgcggc gatccgggtc agccaggtgg
      481 agaagcggga ccgcccggcg aactggccga ggtgggcgta ggcgcggagg taggcctcct
      541 gcatgacgtc ctcgacctcc gcgtcgctcc cgaggatggc gcgcgcgacc cggtacagcc
      601 gctggttgta gcggcgcatc aggacctcga acagcgccgg ctcaccgccg cggacgcggc
      661 ggacgatctc ttcgtcggtg agcgacgcat tcgcgtgcgg cgaaggctcc atgcgatgtc
      721 tccgagagcg ttcattgtag cgacggggtg caggaaagac tctgcgtagt tttgctgcgt
      781 cacgcgcgcc gctgttgcgg actctgtagg gtcgagacca tttgcagaca ttccctgctt
      841 ccgtgacgga ggtcaccgat gcgcatcagc tccaagtgga tcacggccgg cgcggctctc
      901 atgctgtcgc tggcattcgc gggacccggc gggaacgccg gggacaggaa ggacgccagg
      961 aggccggggg tcgaagggcc cgtggcggac aacgcggtga gcatgctggc cgagggacgc
     1021 cggacgttcc ggttcgacac cttcggcgac gaggagttct ggggcgatac gctccgactg
     1081 caccaggcga ttgccgggtc ggccctcgga ggggtcgggc ccggcgtctc gccgcgcacg
     1141 gcgctcgtcg tggggctcaa agtcgattcc gaggcgctcc cgtcgtcgct cgccgagtcg
     1201 ctccgcagag ggcaggtcaa tctggactcc cccgcgacga cggtcgcgct cctgaagctg
     1261 cacgccgtcg tcggggtgac cggcttcttc gatgacggcg ggcggctcgt ctccatgggc
     1321 atccagtgcg ccctgtgcca ctcgacggtg gacaactcgg tcgcccccgg catcggccgc
     1381 cgtctcgacg gctgggcgaa ccgcgatctc gacgtcggca cgatcatcgc gctggctccc
     1441 gatctccagg tgctggccga tctgctcggc gtctcacagg acacggtgcg cacggtcctg
     1501 cgctcctggg gccccggcaa gttcgacgcc gagctgatcc tcgacggcaa ggcgttccgt
     1561 ccggacggca aaccggcggc cacgctcctc ccgcccgcct tcggcctcgc cggcgtcaac
     1621 ctgcacacgt ggaccggctg gggatcggtg acgcactgga acgccttcgt ggccaatctc
     1681 gagatgcacg gaaaggggac cttctacgac ccgcggctca acgatccggt gaagttcccg
     1741 gtggcggcgc gcgccggctt ctccgatgtc cgatccgacg aggatcggat cacgccgcgt
     1801 ctggcggcgc tgcacttcta ccagctcgcc ctgccggccc cccccgctcc cgcggggagc
     1861 ttcgatcggg ccgccgccga tcgcggccgc aacgtgttcc tgggacgcgg ccagtgcgcc
     1921 acctgccacg tcccgccact gttcaccgag ccgggctgga acatgcacac gccggaggag
     1981 atgcacatcg acgacttcca ggcgctgcgc tcccccgacg agcggtaccg cacttcacca
     2041 ctgcgcggcc tgtggaccca cacgaagggg ggcttctacc acgacggccg cttcgcgaac
     2101 ctggaggcgg tcgtcagcca ctacaacacc acgcgcggcc tgaccctctc cgaacaggag
     2161 cgccgcgacc tggtcgaata cctgaagtcg ctctagcggg agacgatcgt ccggcgcgag
     2221 cgcgctccgc agggcgatcg cctcggaggt cggcgggtcc ttggccgtgt cgcgatagtt
     2281 ccgggcgatc tcggcctgga ggttcttcgc tctctgcatt gccagggagt ggtcgccgaa
     2341 gaagaaattg gtgaccttgt tgccgtcgcc gtacttctcg gcgaatttct gccagaggcc
     2401 gggggccttg cccacgtcgt acccggcctc gtagacgtag cgcagcccga cgcggtccgc
     2461 ctggtcttcg taatcgcggc tgtagacgtt gccgacggtg gtgacgccga gaacggtcgc
     2521 cccctgcgcc gccagccggg cccggtcgtc gtcgagcgac tgcgccgcga tggtggccgc
     2581 gacgccggcg atcccctgga tgagccctcc cttcgcctgg cggcggctgt gctcgtgggt
     2641 cgcgtgggcg atctcgtgtc cgagcacgat cgcgagctcg tcgtcgtcga ggtccttcat
     2701 caacccg
//
LOCUS       DASRIQ010000428         7876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_947898, whole genome shotgun sequence.
ACCESSION   DASRIQ010000428 DASRIQ010000000
VERSION     DASRIQ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947898"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFD06_10430"
     CDS             <1..667
                     /locus_tag="VFD06_10430"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HZN03988.1"
                     /translation="CSSEGAALCYRTFCLEAAQRMGTHDHPEVSLHTHSLAEYMRAIR
                     RDDWDEVARLMLSSAEKLASAGADFLVCPDNTLHQAFDLVAARSPLPWLHIAHEVGVE
                     ARRRGFRRPALLGTRWLMTGPVYPKRLEPLELQVLLPSEAERERINSIIFDELVQGRF
                     EAPARAEMVAIIARLKAQGADAAILGCTEIPLLVSQEDSPLPVLDSTRLLARAALREA
                     LAS"
     gene            664..1608
                     /locus_tag="VFD06_10435"
     CDS             664..1608
                     /locus_tag="VFD06_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006529224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZN03989.1"
                     /translation="MKRLRFVTCDVFTDRPFGGNPLAVFPSVEGLPTATMQAIAREFN
                     FSETTFVEKSVRPGCTCKVRIFTPRNELPFAGHPTLGTAHVLVHTGAVPGGAGTTGAP
                     AAAAGAGGPLDLVFEEGVGPVSVRVTLDAGRPVFAELTAAKIPEAGPPPPDAVALARV
                     LSLAPDDLAGGRFTPQAWSAGIPFLFVPLRSRLALERARIDTARFEETLSKYSAPDCF
                     VFAIEAPTEIRARMFAPAEGIEEDPATGSAAAALAGYLAARDESRATTLRWTVHQGVE
                     MGRPSLLKVTAERGPAGLTSVRVGGASVVMTEGEFVLP"
     gene            complement(1615..2172)
                     /locus_tag="VFD06_10440"
     CDS             complement(1615..2172)
                     /locus_tag="VFD06_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN03990.1"
                     /translation="MVRTESTMMPLGTPAPDFQLPDTDGTIVSLDDFRDAKGMLVMFI
                     CNHCPFVKHVRKELALLGRDYRRTVGIVGINSNDIVAFPDDSPEMMKQEEIDAGYIFP
                     YLFDETQEVARDYHAACTPDFFLFDGDRRLVYRGQLDDSRPGNDIPVTGKHLRAAIDA
                     LLAGKPVPSDQKPSLGCNIKWKGAA"
     gene            complement(2262..3443)
                     /locus_tag="VFD06_10445"
     CDS             complement(2262..3443)
                     /locus_tag="VFD06_10445"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZN03991.1"
                     /translation="MTFGGWKRNGVAWLAMVALMALSAAPAGAAIPLLDNEKGKLDME
                     FRFMAWAMDAGPDYPQLPGVNSAPPPPQEENIEDFFLRRLRLVFRGTVTKSLDMYVQF
                     GMDNQGSKIQRDDTGVRIKDAYINYHKWDGLQVVFGQFKVPFLRQNLASGFNQLLVDR
                     SLVPGLRPAIEGSRDVGGMAWGNKGGFQYRTAIFDGSDQEDTNTQSSLRGSARVSYNW
                     FTPEPGLSYTGTTFGEKRILQIGGQADAQNGRVDARDDPAFATEQRAYHAWAADVYYD
                     QPLGGRWAVTAEAAWLQRRDDYDTDGLETRRIEAAYAQAGLLLPWDVGPGRMQVVGRW
                     DEIDTLRGLEETESRSRLFGLTWFTRGHDRKIQFDHGDLHERPTDLDDNFYRLSAVLV
                     F"
     gene            complement(3440..4558)
                     /locus_tag="VFD06_10450"
     CDS             complement(3440..4558)
                     /locus_tag="VFD06_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN03992.1"
                     /translation="MIDIDLILPLEPFTLAVSWRTDERALGVFGPSGAGKTSLLEAIA
                     GLRPGVRGHIRVGGRTWQDSASGFRLPTEERGVGYVPQDALLFPHKDVRANLLTGARR
                     ARQGRGARIDPERVLEILELRGRESAPVTALSGGERQRVALGRALCAGPELLLLDEPL
                     GGLDLPLRRRILPYLLRVEREFGVPTIVVTHDAAEVRLLSHEAIVIENGRVVARGRPD
                     DLFLGGEMLARRGAGGHTNILRGRVLVISDATASVDAGGGVVVSVPVSSGLVRGQDAA
                     VLIGAEELIVAVESPHGLSAQNIVPGVVRDLIGEHPASVAVTVDVACSPTPLVAAVTP
                     QACSRLGLASGRPIYLVWKTHACRAIASGPASSHKEIA"
     gene            complement(4555..5226)
                     /gene="modB"
                     /locus_tag="VFD06_10455"
     CDS             complement(4555..5226)
                     /gene="modB"
                     /locus_tag="VFD06_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HZN03993.1"
                     /translation="MSDGVLAPLLLTLKVALVTTLVIVPLGALAGLLLARRRFAGVSV
                     VETLLALPLVLPPTAVGYMLLRLLARDGPLGVKQLRFDLDLLLTWKGAVLAAAVMSLP
                     LVARTARVAFEEVDPRLETMARTLGLGPLRTFFEITLPLARRGLLAAALLGFARALGE
                     FGATVIVAGNIPGRTQTLALAIFSDLQAGDDRRALWLVGITVALAFAAVYAVEGLLRR
                     RPRFA"
     gene            complement(5223..6035)
                     /gene="modA"
                     /locus_tag="VFD06_10460"
     CDS             complement(5223..6035)
                     /gene="modA"
                     /locus_tag="VFD06_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN03994.1"
                     /translation="MNMARCARFRSVLCAMLMLPAATAGLDAPAVRAAAPAAPIELTV
                     YAAASLKDALNALAPVCGKSAGASLLFNFGASNDLARQIQAANKADVFFSADESWMDQ
                     VAAAGLVDASSRRSLLSNRLVVVTPADSSLAVGAAADLAGPNVKRLSLANPDAVPAGK
                     YAKAWLERAGVWDEVKDKVVPAPDARAALAAVESGAVEAGVVYRTDAGIAKRAKVVFT
                     VPESEGLRISYPVAALEDRPSLAASRKVVDCFSGPEGRAVFERFGFVVLVAR"
     gene            6075..6491
                     /locus_tag="VFD06_10465"
     CDS             6075..6491
                     /locus_tag="VFD06_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN03995.1"
                     /translation="MRRTERGPGSIEWNVAPRWRATRKGAIALGPGKADLLEAIAASG
                     SISAAARALGMSYRRAWVLVATMNACFVRPLVTTTSRRSAGAALTPDGRAALALYRRI
                     EARSLSAARRDIGALLRLLRRPRLTLRARSPTIPAR"
     gene            6488..7234
                     /locus_tag="VFD06_10470"
     CDS             6488..7234
                     /locus_tag="VFD06_10470"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HZN03996.1"
                     /translation="MIREPLVEKGVGTRDGFRWRSREISRVEGFTDAVFAFAVTLLVV
                     SLEVPHTFHDLWEMMSGFFAFAFAFALLVWFWYQQYLYFRRYGLDGAWSIALNAALLF
                     VVLFFVYPLKFLFTILIKMFSGQDARVRLPDGTLHAPISPPEWGPLMTIYGLGYLAVF
                     AIFLLMYLHAWRHRDRLGLDAVERFDTRTSMLEFLLNIGVALVSLAIIQIGGPAWAAV
                     SGLTYAAVGPLMGTLGSIRGIRRDRLRRAS"
     gene            7338..7718
                     /locus_tag="VFD06_10475"
     CDS             7338..7718
                     /locus_tag="VFD06_10475"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN03997.1"
                     /translation="MNPGHPGRIRRVPLLCGAALILSLPGTVWTPAALTGAERLEGMV
                     VDVGGMPSAGAIVSMTGRGLRREALTHDGGGFLIEPVAPGVYLLSAAAGSIASEEVRI
                     EIKDGEAPPHITLTLKPPLPVADP"
BASE COUNT         1115 a         2872 c         2732 g         1157 t
ORIGIN      
        1 ctgcagcagc gaaggggccg ccctctgcta ccgcaccttc tgcctcgaag cagcgcagcg
       61 catggggacg cacgatcacc cggaagtctc cctgcacacc cattcgctgg ccgagtacat
      121 gcgcgccatc cgccgcgacg actgggacga ggtggcgcgc ctgatgctct cgtccgcgga
      181 gaagctggcg agcgccggcg ccgatttcct cgtctgcccc gacaacacgc tgcaccaggc
      241 gttcgatctc gtggcggccc gctcgccgct gccgtggctg cacatcgcgc acgaggtcgg
      301 cgtggaggcg cggcgccgcg gcttccgccg cccggcgctc ctcggtaccc gctggctgat
      361 gaccgggccg gtctacccga agcggctcga gccgctcgag ctccaggtgc tgctgccttc
      421 ggaggcggag cgggagcgga tcaacagcat catcttcgat gagctggtgc aggggcgctt
      481 cgaggcgccg gcacgggcgg agatggtcgc gatcatcgcg cgcctgaagg cgcagggggc
      541 cgacgcggcc atcctcggct gcacggagat tccgctgctc gtgtcgcagg aggactcgcc
      601 gctgccggtc ctcgactcga cgcgcctgct ggcgcgggcg gcgctgcggg aggccctggc
      661 gtcatgaaac gactgcgctt cgtcacgtgc gacgtcttca ccgaccggcc gttcggcggc
      721 aacccgctgg cggtcttccc gtccgtcgaa gggctcccga ccgcgaccat gcaggcgatc
      781 gcccgcgagt tcaacttctc cgagacgacc ttcgtggaga agagcgtgcg ccccggctgc
      841 acgtgcaagg tacgcatctt cacgccgcgc aacgagcttc cgttcgccgg acatccgacc
      901 ctcgggacgg cgcacgtcct ggtgcacacc ggagcggttc ccggcggagc cgggacgacc
      961 ggtgccccgg ccgccgcagc cggcgcgggc gggcccctcg acctcgtctt cgaggaaggg
     1021 gtggggccgg tgtcggtgcg cgtgacgctc gacgcggggc gcccggtctt cgccgagctg
     1081 accgccgcga agatcccgga ggcgggaccg cctcctcccg acgccgtcgc cctggcgcgc
     1141 gtgctctcgc tcgctccgga cgatctcgcc ggcggccgct tcacaccgca ggcctggtcg
     1201 gccggcatcc cgttcctctt cgtgccgctc cgctcgcgcc tggcgctcga gcgggcccgc
     1261 atcgacacgg cgcgcttcga ggaaacgctg tcgaagtact cggcgcccga ttgcttcgtc
     1321 ttcgcgatcg aggcgccgac cgagatccgt gcccgcatgt tcgcgccggc ggaagggatc
     1381 gaggaggacc cggcgaccgg cagcgccgcc gcggcgctcg ccggctacct cgcggcgcgc
     1441 gacgagtcgc gggccacgac gctgcgctgg accgtccacc agggggtcga gatgggacgc
     1501 ccgagtctcc tcaaggtgac cgccgagcgc ggaccggccg gcctgacgtc ggtgcgcgtc
     1561 ggcggcgcct ccgtcgtcat gaccgaaggg gaattcgtcc tcccctgagc gttcttacgc
     1621 cgcgcccttc cacttgatgt tgcagccgag gctcggcttc tggtcggacg ggaccggctt
     1681 cccggcgagg agcgcgtcga tcgcggcgcg cagatgcttg ccggtcaccg ggatgtcgtt
     1741 gccgggacgg ctgtcgtcga gctggccgcg gtagacgagc cggcggtccc cgtcgaacag
     1801 gaagaaatcc ggcgtgcagg cggcgtggta gtcgcgcgcc acctcctgcg tctcgtcgaa
     1861 gaggtacggg aagatgtatc ccgcgtcgat ctcctcctgc ttcatcatct cgggggagtc
     1921 gtccggaaag gcgacgatgt cgttgctgtt gatgccgacg atcccgaccg tccgccggta
     1981 gtcgcggccc aggagcgcca gctccttgcg gacgtgcttc acgaacgggc agtggttgca
     2041 gatgaacatc accagcatcc ccttcgcgtc gcggaagtcg tcgagcgaca cgatcgtccc
     2101 atccgtgtcg ggaagctgga aatccggggc gggggtaccg agcggcatca tcgtggactc
     2161 ggtgcgaacc atggcatctc ctcgtgagct cgtgcggagc agggccgccc cattgtggat
     2221 cgccgacggg cccgcgggcg aacaatcgag ggcgggaaat cctagaagac cagcaccgcg
     2281 gacaggcggt agaagttgtc gtcgaggtcg gtggggcgct cgtgcagatc gccgtggtcg
     2341 aactggatct tgcggtcgtg tcccctggtg aaccaggtga ggccgaagag gcgggaccgg
     2401 gactcggtct cctccagtcc gcggagggtg tcgatctcgt cccagcggcc gacgacctgc
     2461 atgcgccccg ggcccacgtc ccatggcagc aggagacccg cctgcgcgta ggcggcctca
     2521 atccgacggg tttcgaggcc gtcggtgtcg tagtcgtcgc ggcgctgcag ccaggccgcc
     2581 tcggcggtca ccgcccagcg gccgccgagc ggctggtcgt agtacacgtc ggccgcccac
     2641 gcatggtagg cgcgctgctc ggtggcgaag gcggggtcgt cgcgcgcgtc cacgcggccg
     2701 ttctgcgcgt cggcctgccc gccgatctgg aggatgcgct tctccccgaa ggtcgtgccg
     2761 gtgtagctca ggccgggctc cggcgtgaac cagttgtacg acacccgcgc cgagccgcgc
     2821 aggctgctct gggtgttcgt gtcctcctgg tcggagccgt cgaagatcgc cgtgcggtac
     2881 tggaagcccc ccttgttccc ccaggccatc ccccccacgt cgcggctccc ttcgatggcg
     2941 ggacgcagcc ccggcacgag cgaccgatcg acgagcagct ggttgaatcc ggaggccagg
     3001 ttctggcgca ggaacggcac cttgaactgc ccgaagacga cctggagccc gtcccacttg
     3061 tggtagttga tgtaggcatc cttgatccgc acgcccgtgt cgtcgcgctg gatcttgctg
     3121 ccctggttgt ccatgccgaa ctgcacgtac atgtcgagcg acttcgtgac cgtgccccgg
     3181 aagacgaggc gcaggcggcg gaggaagaag tcctcgatgt tctcctcctg cggcggcggc
     3241 ggagcgctgt tcactccggg cagctgggga tagtcggggc ccgcgtccat cgcccacgcc
     3301 atgaagcgga actccatgtc gagcttcccc ttctcgttgt cgagaagcgg aatcgcggct
     3361 cccgccggcg ccgccgacag cgccatgagc gcgaccatcg ccagccacgc caccccgttc
     3421 cgcttccacc ctccgaacgt catgcgatct ccttgtgcga tgaggccggc ccggacgcga
     3481 tcgcccggca ggcgtgggtt ttccagacca gatagatcgg gcggcccgac gcgaggccga
     3541 gccggctgca ggcctgcggc gtgaccgccg cgaccagcgg cgttggcgag cacgcgacat
     3601 ccaccgtgac cgcgacactc gcggggtgct ctccgatcag gtcgcggacc acgccgggga
     3661 cgatgttctg cgccgacagc ccgtgcggcg actccacggc gacgatcagc tcctccgcgc
     3721 cgatgaggac cgccgcgtcc tggccgcgga cgagcccgga cgaaaccggg acgctgacga
     3781 cgacgccccc tcccgcgtcc acggaggcgg tcgcgtcgga gatcaccagc acccgcccgc
     3841 gcaggatgtt ggtgtgcccg ccggcgccgc ggcgcgccag catctctccg ccgaggaaca
     3901 gatcgtccgg cctcccgcgc gccaccacgc ggccattctc gatcacgatc gcctcgtggc
     3961 tcaggagcct cacttcggcg gcatcgtggg tcacgacgat cgtcgggacg ccgaactccc
     4021 gctcgacgcg caggagatac ggcaggatgc gccggcgcag cggcaggtcg agccccccga
     4081 gcggctcgtc gaggaggagc agctccggcc cggcgcacag cgcccggccg agggcgaccc
     4141 gctgccgctc cccgccgctc agcgcggtga ccggcgccga ctcacgcccg cgcagctcga
     4201 ggatctcgag cacccgctcc ggatcgatgc gcgccccgcg tccctgccgg gcccgccgcg
     4261 cccccgtgag caggttggcg cgcacgtcct tgtgcgggaa gaggagcgcg tcctggggga
     4321 cgtagccgac gccgcgctcc tcggtcggga gccggaatcc ggaggcgctg tcctgccacg
     4381 tgcgcccgcc gacgcggatg tggccgcgca ccccgggccg caggcccgcg atcgcttcga
     4441 ggagcgaggt cttgcccgcc ccggaagggc cgaacacgcc gagcgcccgc tcgtcggtgc
     4501 gccaggagac cgcgagggtg aacggctcga gcggcaggat gagatcgatg tcgatcatgc
     4561 gaagcgcggc ctccgccgca agagaccttc gaccgcgtac accgcggcga acgccagcgc
     4621 cacggtgatg ccgacgagcc acagcgcgcg gcgatcgtcc cccgcctgca ggtcgctgaa
     4681 gatggcgagc gccagcgtct gcgtgcggcc ggggatgttg ccggcgacga tcaccgtggc
     4741 gccgaactcg ccgagcgccc gcgcgaagcc gaggagcgcc gccgccagga gcccgcgccg
     4801 cgccagcggc agggtgatct cgaagaaggt gcgcagcggc cccaggccga gcgtgcgggc
     4861 catcgtctcg aggcgcgggt ccacctcctc gaacgccacg cgcgccgtgc gcgccacgag
     4921 cggcagcgac atcaccgccg ccgccagcac cgcccccttc caggtcagca gcagatcgag
     4981 gtcgaagcgc agctgcttca cgcccaaagg gccgtcgcgc gccaggaggc gcagcagcat
     5041 gtatccgacc gccgtcggcg gcagcaccag cggcagcgcc aggagcgtct cgaccacgct
     5101 cacccccgcg aacctgcgcc gcgccaggag cagcccggcg agcgcgccga gcggcacgat
     5161 gacgagagtc gtgaccagcg ccaccttgag ggtgaggagg agcggcgcga ggaccccgtc
     5221 gctcatcggg cgacgaggac gacgaacccg aagcgctcga atacggcgcg tccttcgggg
     5281 ccggagaagc agtccacgac cttccgcgac gcggcgaggg acggacgatc ctcgagcgcg
     5341 gcgaccggat aagaaatcct aagtccctcc gactccggga cggtgaagac gaccttcgcc
     5401 cgcttcgcga tcccggcgtc ggtcctgtac acgacgcccg cctcgaccgc tcccgactcg
     5461 accgccgcca gcgccgcccg cgcgtccggc gccggcacca ccttgtcctt cacctcgtcc
     5521 cagaccccgg ccctctcgag ccaggccttc gcgtatttcc cggccggcac cgcgtcggga
     5581 ttggcgagcg acaaccgctt cacgttcggc ccggccaggt cggccgccgc gccgaccgcc
     5641 agcgacgaat cggcgggtgt cacgacgacg aggcggttgg agagaagcga tcggcggctc
     5701 gatgcgtcga ccagaccggc cgcggccacc tggtccatcc agctctcgtc ggccgagaag
     5761 aacacgtccg ccttgttggc cgcctggatc tgccgggcga gatcgttcga cgcgccgaaa
     5821 ttgaagagca ggctggcgcc ggcggacttg ccgcagacag gagccagcgc gttgagagcg
     5881 tccttcaggc tcgccgccgc gtacacggtc agctcgatgg gcgccgccgg cgccgccgcg
     5941 cgcaccgccg gggcgtcgag cccggccgtc gcggccggga gcatcagcat ggcgcacagc
     6001 acggatcgga accgggcaca tcgggccatg ttcatgagtg gcgcatcata tacggtcgga
     6061 tacaatgcct gtcaatgcga cggacggagc ggggcccggg atcgatcgaa tggaacgtgg
     6121 cgccgcgctg gcgcgccacc cgcaaggggg cgatcgcgct gggccccggc aaggccgatc
     6181 tcctcgaggc gatcgcggcc tcgggatcga tctccgccgc cgcccgcgcc ctcgggatgt
     6241 cgtaccggcg cgcctgggtc ctggtcgcca ccatgaacgc ctgcttcgtc cggccgctgg
     6301 tcaccacgac ctcgcggcgg agcgccgggg ccgcgctcac tcccgacggg cgcgccgcgc
     6361 tggcgctcta ccggcgcatc gaggcacgat ccctttccgc cgcgcgccgc gacatcggtg
     6421 cgctcctgcg cctgctccgc cggccccgct tgacccttcg cgcccgttcc ccgacaatcc
     6481 cggcccgatg atccgcgagc cgctggtgga gaagggggtc ggcacccgcg acgggttccg
     6541 ctggcgcagc cgcgagatct cgcgcgtcga agggttcacc gacgcggtgt tcgcgttcgc
     6601 cgtgacgctc ctcgtcgtct ccctcgaagt cccccacacc ttccatgatc tctgggagat
     6661 gatgagcggg ttcttcgcgt tcgccttcgc gttcgcgctg ctcgtctggt tctggtacca
     6721 gcagtatctc tacttccgcc gttacggcct cgacggcgcc tggtcgatcg cgctcaacgc
     6781 cgccctgctg ttcgtcgtgc tgttcttcgt ctacccgctg aagttcctgt tcaccatcct
     6841 gatcaagatg ttcagcggcc aggatgcgcg cgtgcgcctg ccggacggaa cgctgcacgc
     6901 gccgatcagc cctcccgagt gggggccgct gatgaccatc tacggcctcg ggtatctggc
     6961 cgtgttcgcc atcttcctcc tgatgtacct gcacgcctgg cggcatcgcg accggctcgg
     7021 gctcgacgcg gtggaacgct tcgacacccg cacgtcgatg ctggagttcc tcctcaacat
     7081 cggcgtggcc ctcgtctccc tggcgatcat ccagatcggg gggccggcgt gggccgccgt
     7141 ctcggggctc acctacgcgg cggtcggccc cttgatgggg acgctcggat cgatccgggg
     7201 gatccgccgg gaccgcctcc gacgcgcgag ctgacacttc cgcggccggt acatctacgg
     7261 acgctggttc gggacttcga ccgattgttt cgaggccttc cgcggactac aaaggctcgt
     7321 catcggaagg agcgaggatg aatcccgggc accccggccg gatccggagg gtccctcttc
     7381 tctgcggcgc ggcgctgatc ctgtcgctgc cgggaacggt gtggacgccg gccgccctga
     7441 ccggggccga gcggctcgag gggatggtcg tcgacgtcgg cgggatgccg tcggccggag
     7501 cgatcgtgag catgacgggg cgcgggctcc gtcgcgaggc gctcacccac gacgggggtg
     7561 gcttcctcat cgagccggtg gcccccgggg tctatctcct gagcgcggcc gccggcagca
     7621 tcgcgagcga ggaggtccgc atcgagatca aggacggcga ggcgccgccg cacatcaccc
     7681 tcactctcaa gcccccgttg ccggtggcgg acccatgaca tcgttccacc tcgacgccga
     7741 tcgccggacg gatcgaggtc ccaccggccg gcggacacct cctgaaccac cgcttcgaga
     7801 cagggcggac gccggtctgg ccctcccgga ccacgaccgg cgtccgtcag ttcccgcgct
     7861 ccggctccgg tcggct
//
LOCUS       DASRIQ010000429         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2790737, whole genome shotgun sequence.
ACCESSION   DASRIQ010000429 DASRIQ010000000
VERSION     DASRIQ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790737"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="VFD06_10480"
     CDS             <1..1230
                     /locus_tag="VFD06_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN03998.1"
                     /translation="MALPRHVDRRRFLRDSILLTGAVTAAAASSCRRNQAEAEAAAPA
                     AAAAMPMRLLGRTGLKVSEVGFGGFPIDDPDVVLYALDRGVNYVDTSNCYRGGASETA
                     IGKALKGRRDRFVVTTKWCPHHVGEKPVKQAFLAQLETSLKRLQTDHVDVILNHEVGK
                     NSDGIGLDRLKNPEMFEAFEEAKKSGKARFLGASGHDADLMPIVDYAIGAGPFDVLLC
                     RYSFLDYPEQQSLIEKANAAGVGVIAMKTLAGAKGADLDRFRERHVTFKQAALKWVLS
                     NPKVSNLIISINGTRQVDEYVAAAGKPLTAEDRGILDEYAAEFSTQVCRYSGACLPAC
                     PEGVKIADILRFSMYFHEYGQEQRGLESYARLVEAERAAHCLHCAGYCEQACAYDLPI
                     QSLMIRADAVLAPRRRT"
     gene            complement(1310..>3336)
                     /locus_tag="VFD06_10485"
     CDS             complement(1310..>3336)
                     /locus_tag="VFD06_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZN03999.1"
                     /translation="LTWNGGETWYAPPLPIAQFYHVSVDNATPYHVHGAMQDLGTARG
                     PSDSLADGITNGDWSDVGGGEAGHTASDPKDPNVVWAGEYMGIITRYDHRTGMARHVS
                     IYPENGSGHGAEDLKYRFQWTAPIVVSPHDGNTVYHAGNVLFRTTDGGQTWTAISPDL
                     TRNDKSKQKWSGGPVTGDNTGVEWYCTIFAVAESPKEKGLLWAGSDDGLVHVSRDAGG
                     KWTNVTPRGAPEWGTVSLIEASPHDAGTAYVVYDAHRLDDMKPYLYRTTDYGKSWKSL
                     SSGLPQDVYLHAVREDPKRKGMLYLGTERGVSFSRDDGATWQLLGINFPTVAVHDLQV
                     KNDDLVVGTHGRSIWIFDDLSVIRDWTKEIGDKPVHLFASRPATRWEERSDFHEEGLG
                     ENPPRGAVVSYSLKGKVEGDIRLDIVDANGKVIRTITSKKEAPEVPEDDPDGGPRKKA
                     VLTKEPGVQRVVWNLRTDGALKIKKAKSEGNPEGAIQVPPGSYTARLTVGKTVLTTPV
                     EVLPDPRLGLTPADLEAQKAFVGEIQAQVTRLSNIVNQIQSVRDQLKVKIAALQGSDK
                     AADWVKEAEGVIGKCDAIEAKLHNSKAEVEYDILAKGAMLYSRLTTLLMYTLSGSGAP
                     TQGQREVFDIQRGELDRLDAEWRALLNEQVMEVARKADALAPGGIAVPGR"
BASE COUNT          473 a         1177 c         1127 g          559 t
ORIGIN      
        1 atggccttgc cgcgtcacgt cgaccgccgg aggttcctgc gcgattcgat cctcctcacc
       61 ggcgcggtga ctgcggccgc cgccagctcc tgccggcgga accaggcgga ggcggaggcc
      121 gccgcgccgg ccgcagcagc ggcgatgccg atgcgtctcc tcgggcgcac cgggctgaag
      181 gtctcggagg tcggcttcgg cggcttcccg atcgacgacc cggacgtggt cctctacgcc
      241 ctcgatcgcg gcgtcaacta cgtcgacacc tcgaactgct accgcggcgg ggccagcgag
      301 accgccatcg gcaaggcgct caaagggcgc cgcgaccggt tcgtcgtcac gaccaagtgg
      361 tgcccccatc acgtcggcga gaagccggtg aagcaggcgt tcctggcgca gctcgagacc
      421 agcctgaagc ggctgcagac cgaccacgtg gacgtcatcc tcaaccacga ggtggggaag
      481 aactccgacg ggatcggcct cgatcgcctg aagaacccgg agatgttcga ggcgttcgaa
      541 gaggcgaaga aatcgggcaa ggcgcgcttc ctcggcgcca gcggccacga cgcggacctc
      601 atgccgatcg tcgattacgc catcggggcc gggccgttcg acgtgctcct gtgccgctat
      661 tcgttcctcg actacccgga gcagcaatcg ctcatcgaaa aagccaacgc cgcgggggtc
      721 ggggtgatcg cgatgaagac gctggccggg gcgaaggggg ccgacctcga ccgcttccgc
      781 gagcggcacg tcaccttcaa gcaggcggct ctgaagtggg tcctgtcgaa cccgaaggtg
      841 tcgaacctga tcatctcgat caacggcacg cggcaggtgg acgagtacgt cgccgccgcc
      901 gggaaaccct tgaccgccga ggaccggggc atcctcgacg agtacgccgc ggagttctcc
      961 acccaggtct gccgctacag cggcgcctgc ctgccggcct gcccggaggg cgtgaagatc
     1021 gccgacatcc tgcgcttctc gatgtacttc cacgagtacg ggcaggagca gcgcggcctc
     1081 gagtcgtacg cgcggctggt ggaggcggag cgggcggcgc actgcctgca ctgcgccggc
     1141 tactgcgagc aggcgtgcgc ctacgacctg ccgatccagt cgctgatgat ccgagccgac
     1201 gcagtcctcg ctccccggcg ccggacctga ttccgatctc cgcggaccga gaccggagtc
     1261 cgcgccgacg ccctaccagg cgccccgaag ccgccggcct gtcgaagcct cagcgcccgg
     1321 ggacggcgat cccgcccgga gccagcgcgt cggccttgcg ggcgacctcc atcacctgtt
     1381 cgttcaggag cgcgcgccat tcggcgtcga gccggtccag ctcgccgcgc tggatatcga
     1441 acacctcgcg ctgaccctgg gtcggcgccc ccgagccgga cagcgtgtac atcaggagcg
     1501 tggtgaggcg cgagtagagc atcgccccct tggcgaggat gtcgtactcc acctccgcct
     1561 tcgagttgtg gagcttcgcc tcgatggcgt cgcactttcc gatcacccct tccgcctcct
     1621 tgacccagtc ggccgccttg tcgctcccct ggagcgccgc gatcttcacc ttcaactggt
     1681 cgcgcaccga ctgaatctgg ttgacgatgt tgctgagccg ggtgacctgc gcctggatct
     1741 cgccgacgaa cgccttctgg gcctcgaggt cggccggggt caggccgagg cgcggatcgg
     1801 gcagcacctc gaccggcgtc gtgaggaccg tcttccccac cgtcagccgg gcggtgtagg
     1861 aaccgggcgg gacctggatc gccccttccg ggttcccctc cgacttcgcc ttcttgatct
     1921 tcagggcgcc gtcggtgcgc aggttccaga cgacccgctg cacccccggc tccttcgtga
     1981 ggaccgcctt cttgcgcggc ccgccgtccg ggtcgtcctc cgggacctcc ggagcctctt
     2041 tcttgctggt gatggtgcgg atgaccttcc cgttcgcatc gacgatgtcg agccggatgt
     2101 ccccctcgac cttccccttg agcgagtagg agacgaccgc gccgcgcggc gggttttccc
     2161 cgaggccctc ctcgtggaag tcggagcgct cctcccaccg ggtcgccggc cgcgacgcga
     2221 agaggtgcac cggcttgtcg ccgatctcct tcgtccagtc gcggatgacc gacaggtcgt
     2281 cgaagatcca gatcgaccgg ccgtgcgtcc cgacgacgag gtcgtcgttc ttcacctgga
     2341 ggtcgtgcac cgccacggtc gggaagttga tcccgagcag ctgccaggtg gcgccgtcgt
     2401 cgcgcgagaa ggacacgccg cgctcggtgc cgaggtacag catccccttc cttttcgggt
     2461 cctcgcgcac ggcgtggagg tagacgtcct gggggaggcc ggacgacagg ctcttccacg
     2521 acttgccgta gtcggtcgtc ctgtagaggt acggcttcat gtcgtcgagg cggtgcgcgt
     2581 cgtagacgac gtaggcggtg ccggcgtcgt gcggcgacgc ctcgatgagg ctgaccgtcc
     2641 cccactcggg ggcgccgcgc ggcgtgacgt tcgtccactt cccgccggca tcgcgcgaga
     2701 cgtgcaccag gccgtcgtcg ctgccggccc agagcagccc cttctctttc ggcgactccg
     2761 cgaccgcgaa gatggtgcag taccactcga cgccggtgtt gtcgccggtg accggcccgc
     2821 cggaccactt ctgcttcgac ttgtcgttgc gcgtgagatc cgggctgatg gcggtccagg
     2881 tctggccgcc gtcggtcgtg cggaacagca cgttgccggc gtggtagacg gtgttgccgt
     2941 cgtgcggcga gacgacgatc ggcgccgtcc actggaagcg gtacttgaga tcctccgcgc
     3001 cgtggccact gccgttctcc gggtagatcg acacgtgccg ggccatgccg gtgcggtgat
     3061 cgtagcgggt gatgatcccc atgtactccc cggcccagac cacgttcgga tccttcgggt
     3121 cggaggcggt gtgccccgcc tcgccgccgc cgacgtcgga ccagtcgccg ttggtgatcc
     3181 cgtccgccag gctgtcgctc gggccgcgcg ccgtgccgag gtcctgcatc gcgccgtgca
     3241 cgtggtacgg cgtcgcgttg tccaccgaca cgtggtagaa ctgcgcaatc ggaagcggcg
     3301 gcgcgtacca cgtctcgccg ccgttccagg tgagat
//
LOCUS       DASRIQ010000430         3952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3844776, whole genome shotgun sequence.
ACCESSION   DASRIQ010000430 DASRIQ010000000
VERSION     DASRIQ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3844776"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..655)
                     /locus_tag="VFD06_10490"
     CDS             complement(197..655)
                     /locus_tag="VFD06_10490"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZN04000.1"
                     /translation="MSSKLATLLDDRHVPYRSVTHLADATSQATAQHTHTPGREFAKT
                     VIVWVDGRYAMAVLPAHRRVDLEKLRKWLNAREARLATEQEMSSLFPDCEIGAEPPFG
                     NLYELPVYVSPLLSRDRITFHGGTHEEAVQMKYSDFAALAKPSVIDFVAD"
     gene            1046..1711
                     /locus_tag="VFD06_10495"
     CDS             1046..1711
                     /locus_tag="VFD06_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN04001.1"
                     /translation="MIVIKLESPEIDPRRILIRLVRNVLTLSGASVSTTGEPGYHSFK
                     RSIVLPGRIDAKRISAHCSDRTLSIRIRKAAEPRAARAVREVMIKGVRFVAPETTIRE
                     TAELLRKEDIGSVPVCRDGAVVGILTDRDIAIRATAAGLDPLRTPIAEIMTRNVVTCR
                     EDDRLVDVEQVMHDRQIRRVPVLDRVGSLAGYLTLARIARSESDLRSGHVLRGISQKA
                     ESR"
     gene            complement(1844..2620)
                     /locus_tag="VFD06_10500"
     CDS             complement(1844..2620)
                     /locus_tag="VFD06_10500"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HZN04002.1"
                     /translation="MTERFRLSCVELKYLLGADEIDPFLATLRTTSRDPSIRRDEWRI
                     TTVYLDRRDGFFARAGLNRPERRAEIRLREFFRPGGEPLSPFVWVESKQRNGKSARVH
                     RYQLHRRLVCRLLRGDLDEDQILGCQERFVERDRVRQAAREVRSVAGPGPLVISGATS
                     CLRTALEGGSPVARLTLDRQIRYHLDPAGLEGPDPSLDSATLGPAALEERAGIVEMKY
                     RGGRRPAWCDQRLGLEGPRNYSKFLVLSALSAATTRRPSS"
     gene            complement(2628..>3952)
                     /locus_tag="VFD06_10505"
     CDS             complement(2628..>3952)
                     /locus_tag="VFD06_10505"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HZN04003.1"
                     /translation="PPPPPPPPPTDVNNAAFVSQTVPTTMVAGQSYGVTVRMRNTGNT
                     TWDAPDIFRLGSQNPQDNMTWGMNRVSMPAGSSIAPNQEVTYSWSVTAPATPTTYNFQ
                     WQMKKSGAEWFGALTPNVVVTVTATPPPPPPPPPPPPPPPTATNNAAFVSQTVPATMT
                     AGQTYPVTVRMRNTGNTTWDAPDIFRLGSQNPQDNMTWGMNRVSMPSGSSIAPNQEVT
                     YSWTVTAPATPNTYNFQWQMKKSGAEWFGALTLNVVVTVTATPPPPPPPAGTNAAAYV
                     AQTVPSSMTTGTEYAVSVTMRNTGSTTWTESAQYRLSSRGPRDNSTWGFNRVYLAPGD
                     SISPGESHTFSFDVTAPVTPGNYTFQWSMVQDGVDWFNDVSPAITVTVSDATPAPVPT
                     SLAGASRDPDETWGKCFGAVPAGAGMPWPALLGGVAAMLLSSMLRRRA"
BASE COUNT          630 a         1337 c         1355 g          630 t
ORIGIN      
        1 cggctgagac gtcgtcatgg caccaccccg agaagcttca tggcgcgtcc gcagcagcat
       61 cgcggcggcc gcttcccccc cttgccgggg gaggctcccc gcttcacctg gatctcgcat
      121 ccgcaccggg gatccgagca gacgtacatt tctcccgcgt acagcatcgt cgtccccctt
      181 tggcccgggc cgccggctag tccgcgacga agtcgatcac cgacggcttc gcgagcgctg
      241 caaagtcgct gtatttcatc tggaccgctt cctcgtgggt cccgccgtgg aacgtgatcc
      301 gatcccggga caggagcggc gacacgtaca cgggcagctc gtacaggttg ccgaacggag
      361 gctcggcccc gatctcgcag tccgggaaaa gactggacat ctcctgctcg gtggccagcc
      421 gggcctcccg ggcattgagc cacttcctca gcttttcgag atccacgcgg cgatgggcgg
      481 gcagcacggc catcgcgtac cggccgtcca cccagacgat cacggtcttc gcgaactcgc
      541 gccccggcgt atgcgtgtgc tgcgccgtcg cctgggacgt cgcgtccgcc aggtgagtca
      601 cgctccggta cggcacgtgc cggtcgtcga gcagcgtcgc cagtttggaa ctcaatcgag
      661 tcatgaaccg cctcgctttc ccggcttccc ccgcgcccct gcgaagagcg gccccgaacg
      721 cccggggacc ccttcatgaa tgagagcgcc tggggatcgg cgcgttatct gaaatcgttc
      781 tcaaggcctg agaacgtccg tcacactttc cgccccggtg tttcttccct atggaacgcc
      841 ctgggcgtcc agggtcgacg tatgtctgag gaggaggtgc tgcgtgaaac ggtcagaaca
      901 acccgttcgt cagcctcgcc gcctgtcgcc cctggaacgg atcggacggg gcgtggagcg
      961 catgctccat cccggcaccg aggaaacgcc cccctcttcc cggcgcagct ggctgccgtc
     1021 gatccgcgtg agagagaagg ccggggtgat cgtcatcaaa ctggaaagcc cggagatcga
     1081 tccccgaagg atcctgatcc gcctcgtccg gaacgtcctg acgctgtcgg gagcctcggt
     1141 ctcgacgacg ggcgaacccg ggtaccactc cttcaagaga agcatcgtcc tgccggggcg
     1201 catcgatgcc aagcgcatct cggcccactg cagcgaccgg accctttcca tccgcatccg
     1261 gaaagcggcg gagccgcgcg ccgcccgcgc ggtgcgggaa gtcatgatca agggcgtccg
     1321 gttcgtcgcc cccgagacca ccatccggga gacggcggag ctgcttcgga aagaggacat
     1381 cggttcggtc ccggtgtgcc gggacggcgc cgtcgtgggg atcctcaccg accgcgacat
     1441 cgcgatccgc gccaccgcgg cgggtctgga tcccctccgc acgccgatcg cggagatcat
     1501 gacgcggaac gtggtgacct gccgggaaga tgatcggctg gtcgacgtcg agcaggtcat
     1561 gcacgaccgg cagatccggc gggtgccggt gctcgatcgc gtcggcagcc tggcgggcta
     1621 tctgacgctg gcgaggattg cccggagcga gtccgatctc cgatccggac acgtgcttcg
     1681 cggcatctcg cagaaggcgg agagccgatg atccgacaac tggcggatgg acggtatcgg
     1741 atctactccc ggacgaggaa ctcccggact ggagcgcgga ggaacctcgg gaccttcccc
     1801 tcgctgaaag ccgcgcagcg ccacgagagg gagatccagt tcttcaagag gaagggcggc
     1861 gggtagtcgc cgccgagagc gccgaaagga ccaggaactt cgagtagttc ctcggccctt
     1921 ccaggcccag gcgctggtcg caccaggccg ggcgccggcc tccccggtat ttcatctcca
     1981 cgattccggc ccgctcttcg agcgccgccg gccccagggt cgcggagtcc agcgacggat
     2041 ccggcccctc caggcccgcg ggatcgagat ggtagcggat ctgccggtcc agcgtcagcc
     2101 gggcgacggg gcttcccccc tccagcgccg tgcgcaggca gctcgtcgct cccgaaatga
     2161 ccagcgggcc cggccccgcg acgctccgga cctcccgcgc ggcctgacgc acccggtcgc
     2221 gctcgacgaa ccgctcctgg cagcccagga tctggtcttc atcgagatcg ccccgaagaa
     2281 gacggcagac caggcgtcga tggagctggt agcggtggac gcgggcggac ttcccgttcc
     2341 gctgcttgct ctcgacccag acgaacgggg aaagcggctc ccctcccggg cggaagaact
     2401 cgcggagccg gatctcggca cgacgctccg gacggttcag cccggcccgc gcaaagaatc
     2461 cgtccctccg atccaggtac accgtggtga tccgccactc gtcccgccgg atcgagggat
     2521 cccgcgacgt cgtccgcagg gtcgcgagga acggatcgat ctcgtcggcg cccagcaggt
     2581 acttcaactc cacgcaggag agccggaacc gctccgtcat cagcatctta cgccctccgc
     2641 cgaagcatcg acgacaacag catcgccgcg actccgccca ggagcgccgg ccagggcatc
     2701 cccgccccgg cgggcacggc gccaaagcac ttcccccacg tttcgtcggg gtcgcgggat
     2761 gctcccgcca gcgaagtggg caccggcgcg ggagtggcgt ccgacaccgt gacggtgatc
     2821 gcgggcgaca cgtcgttgaa ccagtcgacc ccgtcctgga ccatgctcca ctggaacgtg
     2881 tagttgccgg gcgtcacggg cgccgtgacg tcgaacgaga acgtatggct ctctcccggc
     2941 gagatcgagt cgccgggggc caggtagacg cggttgaatc cccacgtgga gttgtcccgg
     3001 ggaccgcgcg agctgagccg atactgcgcg gactcggtcc aggtcgtcga gccggtgttc
     3061 cgcatcgtga ccgagacggc gtattccgtc ccggtggtca tgctggaagg aaccgtctgc
     3121 gcgacgtagg cggcggcatt ggtcccggca ggcggcggag gaggcggcgg ggtcgcggtc
     3181 acggtgacga cgacgttcag cgtcagggca ccgaaccact ccgcccccga tttcttcatc
     3241 tgccactgga agttgtaggt gttcggggtg gccggcgcgg tgaccgtcca ggagtaggtg
     3301 acttcctggt tgggcgcgat ggacgagccc gacggcatcg agacgcgatt catcccccac
     3361 gtcatgttgt cctggggatt ctgcgacccg agccggaaga tgtcgggcgc gtcccaggtc
     3421 gtgttgcccg tgttccgcat gcggaccgtc acggggtagg tctggcccgc ggtcatcgtg
     3481 gcgggaacgg tctgcgacac gaacgccgca ttgttggtcg cggtgggagg aggaggcggc
     3541 ggcggcggag gcggcggagg cggtggcggc gtcgcggtca cggtgacgac gacgttgggc
     3601 gtcagcgcgc cgaaccactc cgcaccggac ttcttcatct gccactggaa gttgtaggtc
     3661 gtgggcgtcg caggagcggt gacgctccac gagtacgtga cttcctggtt cggggcgatg
     3721 gacgatcccg cgggcatcga cacgcggttc atgccccagg tcatgttgtc ctggggattc
     3781 tgcgagccca gccggaagat gtcgggggcg tcccaggtcg tgttccccgt gttcctcatc
     3841 ctcacggtga cgccgtagga ctggccggcc accatggtcg tgggcacggt ctgggagacg
     3901 aacgccgcgt tgttgacgtc ggtgggaggc ggcggcggag gaggaggcgg cg
//
LOCUS       DASRIQ010000431         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3570450, whole genome shotgun sequence.
ACCESSION   DASRIQ010000431 DASRIQ010000000
VERSION     DASRIQ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3570450"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VFD06_10510"
     CDS             <1..1031
                     /locus_tag="VFD06_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN04004.1"
                     /translation="LPGHGTIPASLRDVTWKDWYAAVTLAARYTAGRAGTGNPFLAGG
                     HSTGAALLSLYAVRAVSDDTLPRPERLYLVSPAIGISPFAVLTNVVAGLSFLPVFEKS
                     QWIDVRPEYDPYKYNSFPVNAGNQIYSLTHVLHGELLDAVKGGRLDRMPPFLVFQSVV
                     DSTVTASEVVLGLLNLLPPNGSELVAFDVNGREELEGLVNPRFREGLERLRSTAELPF
                     RLTIVGNRRDGSNSIALYTRDAGAAAGGEATAEREEDLPLEWPRGVFSLGHVALPIPH
                     DDPVYGLDPPPDAVPRYPLGAFPGRGEAGALLVPLDAFARLRCNPFFSVIRARIVESL
                     TKPETAAR"
     gene            complement(1056..>2256)
                     /locus_tag="VFD06_10515"
     CDS             complement(1056..>2256)
                     /locus_tag="VFD06_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007979453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN04005.1"
                     /translation="VLAVLVFAAWITVPWAFQAPRRERPVPADRTTEAVIPGIPNARY
                     YVLEDIEPFVRDVLAARDREAAYLAKSGHTGPLPPAELLAVSGGGDKGAFGAGFMNGW
                     TAKGTRPVFKGVTGVSTGALIAPFAFLGTDYDRVLKEVYTSVRPADISRRRWFLAAVS
                     NDALADNAPLWTLISKHIDDSLLARIAEEYDHGRLLYIGTTNLDARQPVIWNMGAIAA
                     SKSPNALALFRSILLASAAIPGAFPPSMVPVEVDGKKYEEMHVDGGASAQVFLYPPSM
                     PRAAEAMGVQMNRGGHVYVIRNSVLTATWADVERRTISIAGRAIDSLIHTQGIGDLYR
                     IYLTTQRDGFDFNLVYIGPDFKYENKKEEFETPFMVSLYDYGFRMGQEADPWKKTPPG
                     FDAPLGK"
BASE COUNT          314 a          791 c          784 g          367 t
ORIGIN      
        1 ggcttcccgg ccacggcacc atcccggcat cgcttcggga cgtcacctgg aaggactggt
       61 acgccgccgt gaccctcgcc gcccgctaca ccgcgggtcg cgccggaacg gggaacccgt
      121 tccttgccgg ggggcactcg accggcgcgg cgcttctcag cctctacgcg gtgcgcgccg
      181 tctccgacga caccctgccg cgccccgagc gcctgtacct cgtgtcgccg gcgatcggga
      241 tctcgccgtt cgccgtcctc accaacgtcg tcgccggcct gtcgttcctg ccggtgttcg
      301 agaagtcgca gtggatcgac gtccggcccg agtacgaccc gtacaagtac aactccttcc
      361 cggtgaacgc ggggaaccag atctattccc tgacgcacgt cctgcatggc gagctgctcg
      421 acgcggtgaa gggagggcgg ctcgaccgga tgcctccttt cctcgtgttc cagtcggtcg
      481 tcgattccac ggtgaccgcg tcggaggtgg tgctcgggct cctgaatctg ctgccgccca
      541 acgggagcga gctcgtggcg ttcgacgtga acggccggga ggagctggaa gggctcgtga
      601 atccccgctt ccgcgaaggg ctggagcgcc tgcgctccac tgcagagctg ccgttccggc
      661 tgaccatcgt cggcaaccgg cgggacgggt cgaattccat cgcgctctac acccgcgacg
      721 ccggcgccgc ggccggcggc gaagcgacgg cggagcgtga ggaggacctg ccgctcgagt
      781 ggccgcgcgg cgtcttctcg ctcgggcacg tggcgctgcc gatccctcac gacgatccgg
      841 tctatggcct cgatccgccg cccgacgccg tgccgcgcta ccccctcggc gcgttccccg
      901 ggcgcggcga ggccggggcg cttctcgtcc cgctcgacgc gttcgcgcgg ctgcgctgca
      961 atccgttctt ctccgtcatc cgcgcccgca tcgtcgagag cctgacgaag ccggagacag
     1021 cggcccgctg atcccggcgc cagccgctcc ccggcctact tcccgagagg cgcgtcgaac
     1081 cccggtggcg tcttcttcca cgggtccgcc tcctgtccca tgcggaaccc gtagtcgtag
     1141 agcgacacca tgaacggcgt ctcgaactcc tccttcttgt tctcgtactt gaagtcgggg
     1201 ccgatgtaga cgaggttgaa gtcgaacccg tcccgctggg tcgtcaggta gatgcggtac
     1261 aggtcgccga tcccctgggt gtggatgagg gagtcgatgg cgcgccccgc gatgctgatg
     1321 gtgcggcgct ccacgtcggc ccaggtggcc gtgagcacgg agttgcggat gacgtagacg
     1381 tggccgccgc ggttcatctg cacccccatc gcctccgcgg ccctgggcat gctcggcgga
     1441 tagaggaaga cctgggccga ggcgccgccg tccacgtgca tctcctcata cttctttccg
     1501 tccacctcga ccgggaccat cgaggggggg aaggcgcccg ggatcgcggc ggaggcgagc
     1561 aggatcgagc ggaacagggc gagggcgttc ggcgacttgc tggccgcgat cgcccccatg
     1621 ttccagatga ccggctggcg cgcgtcgagg ttggtggtgc cgatgtagag gagccgcccg
     1681 tgatcgtatt cctcggcgat ccgcgccagg aggctgtcgt cgatgtgctt cgagatcagc
     1741 gtccacagcg gcgcattgtc ggccagggcg tcgttcgaga ccgccgcgag gaaccagcgg
     1801 cgccgcgaga tgtcggccgg acgcacggag gtgtagacct ccttgagcac gcggtcgtag
     1861 tcggtgccga ggaacgcgaa cggcgcgatg agggcgccgg tgctgacgcc ggtcaccccc
     1921 ttgaagaccg gccgtgtccc cttggcggtc cagccgttca tgaagccggc gccgaacgcc
     1981 cctttgtctc cacctcccga cacggccaaa agctcggcgg ggggcagggg accggtgtgg
     2041 ccgctcttcg ccaggtacgc cgcttcgcgg tcccgcgccg ccaggacgtc ccggacgaac
     2101 ggttcgatgt cctccaggac gtagtaccgg gcattcggga tcccggggat gacggcctcg
     2161 gtggtgcggt cggcggggac cggccgctcc cgccgcggcg cctggaaggc ccatggcacc
     2221 gtgatccagg cggcgaacac caggacggcg agaacg
//
LOCUS       DASRIQ010000432         4504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_181252, whole genome shotgun sequence.
ACCESSION   DASRIQ010000432 DASRIQ010000000
VERSION     DASRIQ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181252"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFD06_10520"
     CDS             <1..425
                     /locus_tag="VFD06_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04006.1"
                     /translation="TFDIVMGAVDAWLSSEARIATQMYGMAAPGEGAADGGGRPMLKA
                     MLDCGRSSGGVTVRSGLIGAGVMLGATFLFSVVGIVLRRAGFPVAGEILKGLAFTGPF
                     VLSMPFWLMRGTPWRAQAAIVGITFAILLAASYIAVKI"
     gene            complement(466..813)
                     /locus_tag="VFD06_10525"
     CDS             complement(466..813)
                     /locus_tag="VFD06_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN04007.1"
                     /translation="MSPDLGAASQVRDAAPLFAALGDETRFGLLVRLASSGPGSIARL
                     SARAQVSRQAITKHLEILNEAGLVTSARRGRERIWQIEPDRLAEARRYLDLISRQWDD
                     ALGRLKRFVEAPD"
     gene            complement(816..1274)
                     /locus_tag="VFD06_10530"
     CDS             complement(816..1274)
                     /locus_tag="VFD06_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN04008.1"
                     /translation="MTKTETDRIEKQIVLRAPLARVWKALTDAEEFGTWFRVRLKGPF
                     AAGRRVTGQITYPGYEHVTMDVTVERMESERLFSFRWHPGAVDPKMNYDKEPATLVEF
                     RLEKADGGTRLTVVESGFDAIPPSRRDEAFRMNSEGWTIQVENIRRHVDG"
     gene            complement(1382..1858)
                     /locus_tag="VFD06_10535"
     CDS             complement(1382..1858)
                     /locus_tag="VFD06_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZN04009.1"
                     /translation="MSPRRSAGLLLYRESPSGIEVLLVHPGGPFWAKKDDGAWTIPKG
                     LIEAHEDPLAAARREFEEETGLVPPSTDAIPLDPLRQPGGKIVHAWAVLGGDFDPAGL
                     RSNTFTMEWPPKSGAQKTFPEIDRAAWFPLDAARLKILQGQAGFLDQLEKRLGRAG"
     gene            complement(1881..2315)
                     /locus_tag="VFD06_10540"
     CDS             complement(1881..2315)
                     /locus_tag="VFD06_10540"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN04010.1"
                     /translation="MIDPEHHRRLERMYVSAPVTQWYGAAIGISDGAAEVRVETRPEF
                     LHAAHGVHGSVYFRLLDDAAFFAVNSRVLEVFVLTVSFTVQFARPVSSGLLRARGRVL
                     HGGGRLFFAESDLIDANGDLLGHGSGVFSRSAIPLDARVGYA"
     gene            complement(2312..3106)
                     /locus_tag="VFD06_10545"
     CDS             complement(2312..3106)
                     /locus_tag="VFD06_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN04011.1"
                     /translation="MSNPAQGYEAYMVPALFRPWAERLVGRAGPRQGERVLDVGCGTG
                     IVAREVAALLGQKGSVAGMDANPHMLEVARAAAAREQVSIEWFEGRAERLPFPDGRFD
                     LVLCQFALMFFTDRPAALAEMRRVLTHTGRVAVGVWQGLDRHPFYRTLHEVIQRRAGV
                     SALELIFSLGSRDEVEALLKSAGFGRIDIEPVSMTTRFPDPEAFLAGEIEVDTAAIPS
                     MQHLDEGARRSLVAAISVEMKSPLAEITEGNHVVIPFHAHLATASA"
     gene            complement(3103..3468)
                     /locus_tag="VFD06_10550"
                     /pseudo
     CDS             complement(3103..3468)
                     /locus_tag="VFD06_10550"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(3487..3939)
                     /locus_tag="VFD06_10555"
     CDS             complement(3487..3939)
                     /locus_tag="VFD06_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04012.1"
                     /translation="MKRRFPRTLWYGLAVVLAAPILFLALFIRFPVTRDVPWATLLMF
                     AVGLGLLTHSLRHAYKDAAIYRGKVAVPIALVFGIALAGLFSWTLLVQARQIPVALGA
                     PRPGQMAPDFTLPDQDGQPVSLARLLQAPLDATRKANGVVLVFYRGYW"
     gene            complement(3949..>4504)
                     /locus_tag="VFD06_10560"
     CDS             complement(3949..>4504)
                     /locus_tag="VFD06_10560"
                     /inference="COORDINATES: protein motif:HMM:NF016842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NMD3-related protein"
                     /protein_id="HZN04013.1"
                     /translation="RSDRKPPLARLSERLPDGTMCERCGAVYGRRHWRAGTEAERAMA
                     QGFIWATCPACHQVQSGEYYGSVTIRGARAIREEDAIRRRIDNVGARAQYTQPERRLV
                     SIERRGDTIEVLTTSQKLAHRIVNALLAAFGGHGTYSWSDRDGFLRATWLWDDEPAPR
                     RQPDSKRRQPGSKRQQASSKRKKR"
BASE COUNT          690 a         1463 c         1671 g          680 t
ORIGIN      
        1 tgacgttcga catcgtgatg ggggcggtcg atgcatggct gtcgtcggaa gcacgcatcg
       61 cgacgcagat gtacggaatg gccgcgcccg gtgaaggcgc ggcggacgga ggggggagac
      121 cgatgctgaa agcgatgctg gactgcgggc gatcgagcgg cggcgtgacg gtgcgcagcg
      181 ggctcatcgg cgcgggggtg atgctgggcg cgacgttcct cttctccgtt gtgggcatcg
      241 tcctgcggcg cgccggcttt ccggtcgcgg gggagatcct caaaggcctg gcgttcaccg
      301 gaccgtttgt tctctcgatg ccgttctggc tgatgcgcgg gaccccgtgg cgggcgcagg
      361 ccgccatcgt cgggatcacg ttcgcgatcc tcctcgcggc gagctacatc gcggtgaaga
      421 tctaggctcg cgcgctcacg tcctggtccg gtgcgtcgct gcggcctagt ccggcgcctc
      481 gacgaagcgc ttcagccggc cgagggcgtc gtcccactgc cgggagatga gatcgagata
      541 gcggcgcgct tcggcgagcc ggtccggctc gatctgccag atgcgctcgc ggccgcggcg
      601 ggcggaggtc acgagcccgg cctcgttcag gatctcgagg tgcttggtga tcgcctggcg
      661 cgacacctgc gcccgggcgc tgaggcgggc gatcgagccg gggccgctcg acgcgagccg
      721 gacgagcagc ccgaaccggg tttcgtcgcc gagagcggcg aagaggggcg ccgcgtcccg
      781 gacctgggac gcggcgccga gatcggggga cacgcttacc cgtccacgtg ccggcggatg
      841 ttctcgacct gaatggtcca gccctcgctg ttcatccgga acgcctcgtc gcggcggctg
      901 ggcgggatcg cgtcgaagcc ggattcgacg accgtgaggc gggtgccgcc gtcggccttc
      961 tcgaggcgga actcgacgag cgtcgccggc tccttgtcgt agttcatctt cgggtccacg
     1021 gcgcccgggt gccagcggaa cgagaacagg cgctcggact ccatcctctc gaccgtgacg
     1081 tccatggtga cgtgctcgta gccggggtag gtgatctgac cagtgacgcg ccggccggcg
     1141 gcgaacggcc ccttcagacg cacgcggaac caggtgccga actcctcggc gtcggtcagc
     1201 gccttccaga cacgcgccag tggggcgcgc agcacgatct gcttctcgat gcggtcagtc
     1261 tcggtcttcg tcattggcaa cctcctggtt gcatattacg acgacgaggc cgaaaaggca
     1321 actaaaaagt tgcatgtttg gcccgcggcc ggtccgggcg tcgggccgcc cgccacggcc
     1381 gtcagccggc ccggccgagc cgtttttcca gctggtccag gaaaccggcc tgtccctgga
     1441 ggatcttcag gcgggcggcg tcgaggggga accaggcggc ccggtcgatc tccgggaagg
     1501 tcttctgcgc gcccgatttc ggcggccact ccatcgtgaa ggtgttgctg cgcagaccgg
     1561 ccggatcgaa atcgccgccg agcaccgccc aggcatggac gatctttcct ccgggctgac
     1621 gcagcgggtc gagcgggatg gcgtcggtgg aggggggcac gaggccggtc tcctcctcga
     1681 actcgcggcg ggcggcggcg agcgggtcct catgcgcttc gatgaggcct ttggggatgg
     1741 tccaggcgcc gtcgtccttc ttcgcccaga aggggccgcc gggatggacg aggagcacct
     1801 cgatcccgga gggcgactcc cggtagagca gcagaccggc actgcgcctc ggcgacattc
     1861 aggtccgcgg cgctccgcgc tcaggcatac ccgacccgtg cgtcgagcgg gatggcgctg
     1921 cgggagaaga cgccgctgcc gtggccgagg aggtccccgt tcgcgtcgat gagatcggat
     1981 tcggcgaaaa agaggcggcc gcccccgtgg aggacgcgac cgcgcgcgcg caagaggccg
     2041 ctcgagaccg ggcgggcgaa ctgcaccgtg aacgacacgg tgaggacgaa cacctcgagg
     2101 acgcgtgaat tcacagcgaa aaacgccgca tcgtcgagaa gccggaagta gaccgatccg
     2161 tggacgccat gcgcggcatg caggaattcc ggccgggtct cgacccggac ctcggcggcg
     2221 ccgtcggaga tgccgatcgc ggcgccgtac cactgcgtga ccggggccga gacgtacatg
     2281 cgctcgagcc tgcggtggtg ctcggggtcg atcatgccga ggcggtcgcc aggtgcgcgt
     2341 ggaacgggat gaccacgtgg ttcccctcgg tgatctcggc gagcggcgac ttcatttcga
     2401 cggagatggc ggcgacgagc gatcggcggg cgccctcgtc gaggtgctgc attgacggga
     2461 tggcggcggt gtccacttcg atctctccgg cgagaaacgc ctccgggtcg gggaaacggg
     2521 tggtcatcga gaccggctcg atgtcgatgc ggccgaagcc cgcgctctta aggagtgctt
     2581 cgacctcatc gcggctgccg agggagaaga tcagctcgag ggccgagacc ccggcgcgtc
     2641 gctggatcac ttcgtgaagc gtgcgataga aaggatgccg gtccagcccc tgccagacgc
     2701 cgacggccac gcgacccgtg tgggtcagca cgcggcgcat ctcggcgagg gccgccggcc
     2761 ggtccgtgaa gaacatcagc gcgaactggc agaggacgag atcgaagcgg ccgtcgggga
     2821 aggggaggcg ctccgcccgc ccctcgaacc actcgatgga gacctgctcg cgcgcggccg
     2881 cggcgcgcgc gacctcgagc atgtgcggat tggcgtccat ccctgcgacc gagcccttct
     2941 gtccgaggag cgcagccact tcgcgggcga cgatgccagt gccgcacccg acgtcgagga
     3001 cccgctcacc ctgacgcgga ccggccctgc ccaccagtcg ctccgcccag ggacgaaaca
     3061 gcgccggcac catgtaggct tcatagcctt gcgctggatt gctcatcgtc gttcctctgc
     3121 attgttcacc gtcgtgtcct ccgctagggc ctcgtcgatc gccttcagga tctgctcggg
     3181 cgtggcgcgc acggcatacg acggcgtgag gttcgcccat cggacggtgc cggcggcgtc
     3241 gatgaagaac tcggcggggc gggcgatgtc ggtcccgccc ggcccggcgc gaggatggag
     3301 caggtcgtag cttcggatca cagagccggt cgggtcgccg aggaacgtga aggtgtaacc
     3361 ggtcttctgg cggagatcgc ggttcgtctc gggggaatcg acgctgatgg ccacgaggcg
     3421 cacgccgcgg gcggtgaatt cgggaagatg ggcttgcaga ctccgtaact cggagttgca
     3481 gagcgatcac cagtagccgc ggtagaagac gaggaccacg ccgttcgcct tccgtgtcgc
     3541 gtcgagcggg gcctgcagca gccgcgccag cgagaccggc tggccgtcct ggtcggggag
     3601 cgtgaagtcg ggagccatct gccccggacg cggcgcgccg agcgccacgg ggatctgtcg
     3661 cgcctggacc agcagggtcc aggaaaagag gccggcgagc gcgatgccga acaccagcgc
     3721 gatcggcacg gccaccttgc cccggtagat cgccgcgtct ttgtaggcgt ggcgcagact
     3781 gtgggtcagg agcccgaggc cgacggcgaa catcaacagt gtcgcccacg ggacatcgcg
     3841 cgtcaccggg aagcggatga agagcgcgag gaacaggatc ggcgccgcca ggacgacggc
     3901 gagcccgtac cagagggtgc gcggaaaccg gcgcttcacg cgcgaaggtt accgcttttt
     3961 tcgcttcgag gaagcctgct gccgtttcga gccgggctgc cgccgcttcg agtcaggctg
     4021 ccgacgcggg gccggctcgt cgtcccagag ccaggtggcg cggaggaagc cgtcgcggtc
     4081 ggaccacgag tacgtcccgt gaccgccgaa ggcggcgagg agcgcgttga cgatccggtg
     4141 cgcgagcttc tgcgacgtgg tcagcacctc gatggtgtcg ccgcggcgct cgatcgagac
     4201 gaggcgccgc tcgggctggg tgtactgcgc gcgggcgccg acgttgtcga tgcggcggcg
     4261 gatggcatcc tcttcccgaa tcgcgcgggc gccgcggatg gtcacagagc cgtagtactc
     4321 gccggactgg acctggtggc acgcgggaca ggtcgcccag atgaatccct gcgccatcgc
     4381 ccgctcggcc tccgtaccgg cacgccagtg gcgacggccg tagacggcgc cgcagcgctc
     4441 gcacatcgtc ccgtcgggca gacgctccga gaggcgcgcg aggggcggct tgcggtcgct
     4501 cctc
//
LOCUS       DASRIQ010000433         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2502192, whole genome shotgun sequence.
ACCESSION   DASRIQ010000433 DASRIQ010000000
VERSION     DASRIQ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2502192"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="VFD06_10565"
     CDS             complement(<1..1672)
                     /locus_tag="VFD06_10565"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN04014.1"
                     /translation="MARRFARRPAAGRRAVLVAALLGAFVCATTPLRAQEGAKGPGSD
                     AQRQAGQKLYDKYCSQCHGEKGDGQGPAAVHLLPKPRDFTAGKYKFRTTPNGALPTHD
                     DIVSIIKRGMPYTSMPAWPDFSAEELSNLAYYVASFSPDFANPELVPKAIELKSGKGS
                     SKESVEEGKKAYEANGCLGCHGAAGRGNGSSAPTLKDDLGHPLKPADLTQRWTFRGGG
                     TREDIFRTLSTGLNGTPMPSYVEAVPDEQRWAIVDFIDSLSAAEPNYATLVRAPAIDE
                     PIDLQRAGEQFKNAPTARFPIVGQIMEPGRSFHPPAVSILVEAIHDSDSLAVRLRWHD
                     MRADTTGHNAPDLRVPIEEEKESGAPAAPTEEGGDIWGEEAAPAAPAAPAAEGDVWGE
                     ASADAAPAAGPASEFSDAAAVQWPLAMGATARKPYFIFGDATNPVDLWFFDLAKKEPR
                     QYTGKGSGNLTPSDTGELQGLAHYDDGEWTVVYKWPLRPTIGVPLNEGQFVPVAFSVW
                     DGMTRERGNRRALTVWYHLYVEPTAVVSPVVPMLKTAGFVLLLEVIAIF"
     gene            complement(1672..2505)
                     /locus_tag="VFD06_10570"
     CDS             complement(1672..2505)
                     /locus_tag="VFD06_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZN04015.1"
                     /translation="MNWAALLPVVIAFGLLAWRRVGLLAWALAWWAGIYVALRWGFAV
                     PIPFSVLSIYMGIVTLAILAFVTSSAERRQQVADPLVRLIVEPRFRLVLAALVVLIPA
                     AAAANVYMQAKAPLEAPFFARTIHPASPGSITVHEKTFDLDAGENPFRELETSNPEEF
                     RKHVAEGKRVYYENCVFCHGDNLKGNGMYIHGLQPIPTNFADSGTIPMLRETFLFWRI
                     AKGGPGMPEEGGPWDSAMPAWEKFLQEEDLWNVILFLYDFTGSRPRARESVHDEVEKG
                     H"
     gene            complement(2502..2996)
                     /locus_tag="VFD06_10575"
     CDS             complement(2502..2996)
                     /locus_tag="VFD06_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZN04016.1"
                     /translation="MRVPLIPRLALLVLATTAFYTYVGQMVPQKEVHPPVEIVLKKDM
                     DTAEMVKVGREIMDGKGLCFTCHTIGKKGALRFPDLENVDARAKTRIPGLDDIGYFAQ
                     SMYEPDHFIVQGFNPGMPVINKPPIGLTDEEILTVIAYLQSLGGSPTVTMETKHQYNK
                     GGGS"
     gene            complement(2996..>3637)
                     /locus_tag="VFD06_10580"
     CDS             complement(2996..>3637)
                     /locus_tag="VFD06_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04017.1"
                     /translation="AHPILGFLGVMSAKNTAVNILILTTFMSFLLYRRTGKVATVAWA
                     KAGNAIQLAIFAAASVFVIFLGIYGYFVEATVRIGLSVPQVGSVLLAMVSITAIDVFL
                     FRGARSTGEVRWGKIPAASQYVLIFIAVTFTWLMGLMGYVRSGLRQHWHVYGVVRDTS
                     VDAFTPTLGFATMVVSCTVLAFFLLIGFVFWLASLHDKKDWGEPEGTAAGGRH"
BASE COUNT          569 a         1237 c         1260 g          571 t
ORIGIN      
        1 agaagatggc gatcacctcg agcagcagca cgaacccggc ggtcttgagc atcggcacga
       61 cgggggagac gaccgccgtc ggctcgacgt acaggtggta ccagacggtc agggcgcggc
      121 ggttgccgcg ctcccgggtc atcccgtccc acaccgagaa cgccaccggc acgaactgcc
      181 cttcgttgag cggcacgccg atcgtgggac ggagcggcca tttgtacacg accgtccact
      241 ccccgtcgtc gtagtgcgcc aacccctgga gctcgccggt gtcgctcggc gtcaggttcc
      301 cggatccctt gccggtgtac tgccgcggct ccttcttcgc caggtcgaag aaccagagat
      361 cgaccgggtt ggtggcgtcg ccgaagatga agtacggctt gcgggccgtc gcccccatcg
      421 ccagcggcca ctgcacggcg gccgcgtcgg agaactccga cgccggaccg gcggccgggg
      481 ccgcgtccgc ggacgcctct ccccacacgt ccccctccgc cgccggggcc gccggagcgg
      541 cgggcgccgc ctcctcgccc cagatgtcgc cgccctcttc ggtcggcgcg gccggcgccc
      601 cggactcctt ctcctcctcg atcggcaccc tcaggtcggg ggcgttgtgg cccgtcgtgt
      661 cggcccgcat gtcgtgccag cgcaggcgca ccgccaggct gtcggagtcg tggatcgcct
      721 ccaccaggat cgacaccgcc ggcgggtgga acgaccggcc cggctccatg atctggccga
      781 cgatcgggaa gcgtgcggtc ggggcgttct tgaactgctc ccccgcgcgc tgcagatcga
      841 tcggctcgtc gatcgccggg gcgcgcacga gcgtggcgta gttcggctcc gcggccgaga
      901 gggagtcgat gaaatcgacg atcgcccagc gctgctcgtc cgggacggcc tccacgtacg
      961 acggcatcgg cgtgccgttc agcccggtgc tcagcgtccg gaagatgtcc tcgcgggtgc
     1021 cgccgccgcg gaaggtccag cgctgcgtca gatcggccgg cttgagcgga tgccccaggt
     1081 cgtccttcaa ggtcggagcg gaggaaccgt tgccccgccc cgcggccccg tggcagccga
     1141 gacagccgtt cgcctcgtac gccttcttcc cctcctccac cgattccttg ctcgagccct
     1201 tgcccgactt cagctcgatc gccttcggga cgagctccgg gttggcgaaa tcgggggaga
     1261 acgacgcgac gtagtaggcg aggttgctca gctcctcggc ggagaagtcg ggccaggccg
     1321 gcatcgaggt gtagggcatc ccgcgcttga tgatgctgac gatgtcgtcg tgcgtcggca
     1381 gggctccgtt cggtgtcgtg cggaacttgt acttgccggc ggtgaagtcg cgcggcttcg
     1441 ggagcagatg gaccgccgcc ggaccctggc cgtccccctt ctcgccgtgg cactgcgagc
     1501 agtacttgtc gtagagcttc tgcccggcct ggcgctgcgc gtcggagccc ggccctttcg
     1561 ccccttcctg ggcgcggagc ggcgtcgtgg cgcagacgaa ggctccgagg agggcggcca
     1621 cgaggacggc gcggcgtccg gctgccgggc ggcgtgcgaa gcggcgcgcc atcaatgccc
     1681 cttctccact tcgtcatgaa ccgattccct ggcgcgcggc cggctcccgg tgaagtcgta
     1741 gaggaagagg atgacgttcc acagatcctc ctcctggagg aacttctccc aggccggcat
     1801 ggcgctgtcc cacggcccgc cttcctccgg catcccgggc ccccccttgg cgatgcgcca
     1861 gaacaggaag gtctcacgca gcatgggaat ggtgccgctg tcggcgaagt tcgtcgggat
     1921 cggctgcagg ccgtggatgt acatgccgtt ccccttcaag ttgtcgccgt ggcagaagac
     1981 gcagttctcg tagtagaccc gcttcccctc cgcgacgtgc ttccggaact cctcggggtt
     2041 cgaggtctcc agctcgcgga acgggttctc tccggcatcg aggtcgaacg tcttctcgtg
     2101 caccgtgatc gagccggggg aggccgggtg gatggtgcgc gcgaagaacg gcgcctcgag
     2161 cggcgccttg gcctgcatgt agacgttcgc cgccgccgcg gccgggatca ggacgacgag
     2221 cgcggcgagg acgagccgga agcgcggctc gacgatgagg cgcacgagcg gatcggcgac
     2281 ctgctgccgc cgctcggcgc tcgaggtgac gaaggcgagg atggcgagag tgacgatccc
     2341 catgtagatc gacagcaccg agaacgggat cggaaccgcg aatccccagc gcagcgcgac
     2401 gtagatgccg gcccaccagg cgagggccca ggcgagcaga ccgacccggc gccacgcgag
     2461 gagcccgaag gcgatcacga ccgggaggag ggccgcccag ttcacgagcc gccccctttg
     2521 ttgtactggt gcttggtctc catcgtgacg gtcggcgaac cgccgaggct ctgcagatag
     2581 gcgatgaccg tgaggatctc ctcgtcggtc aggccgatcg gcggcttgtt gatcaccggc
     2641 atgccggggt tgaagccctg gacgatgaag tggtcgggct cgtacatcga ctgcgcgaag
     2701 tacccgatgt catcgagccc ggggatgcgc gtcttcgccc gtgcatccac gttctcgagg
     2761 tcggggaagc gcagcgcccc cttcttgccg atggtgtggc aggtgaagca gagccccttg
     2821 ccgtccatga tctcccgccc gaccttgacc atctcggcgg tgtccatgtc cttcttgagg
     2881 acgatctcga cgggaggatg gacctccttc tgcggcacca tctgcccgac gtaggtgtag
     2941 aaggcggtcg tcgccaggac cagcagggcc agtcggggga tgagcgggac gcgcatcagt
     3001 gccggccccc cgcggccgtc ccttccggct cgccccagtc cttcttgtcg tgcaggctcg
     3061 ccagccagaa cacgaacccg atcagcagga agaaggcgag cacggtgcag gacacgacca
     3121 tggtggcgaa ccccagggtc ggggtgaagg cgtccaccga cgtgtcgcgg acgaccccgt
     3181 acacgtgcca gtgctgccgc aggccggagc ggacgtaccc catgagcccc atcagccagg
     3241 tgaatgtgac ggcgatgaag atgaggacgt attgcgacgc ggccgggatc ttcccccagc
     3301 gcacctcacc cgtgctgcgg gctccgcgga acaggaacac gtcgatcgcg gtgatcgaga
     3361 ccatggcgag caggaccgaa ccaacctggg ggaccgacag cccgatgcgc accgtcgcct
     3421 cgacgaagta gccgtagatg ccgaggaaga tcacgaacac ggaggcggcg gcgaagatcg
     3481 ccagctggat ggcgttcccg gccttcgccc aggcgacggt cgccaccttg ccggtccggc
     3541 gatagaggag gaagctcatg aacgtcgtga ggatcaggat gttcacggcg gtgttcttcg
     3601 ccgacatcac gccgaggaag cccagaatcg ggtgcgc
//
LOCUS       DASRIQ010000434         8272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1734466, whole genome shotgun sequence.
ACCESSION   DASRIQ010000434 DASRIQ010000000
VERSION     DASRIQ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734466"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFD06_10585"
     CDS             complement(<1..364)
                     /locus_tag="VFD06_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN04018.1"
                     /translation="MTGRIESSGCAAILPLLPLHFYSGLETADRLRVEAHLSACGECA
                     AAWEETRRALAAVDSGAAFPREREVDWDDFARRTVARARAAEPAAAAPETPGRRRSPW
                     MWPAGVAAAAASLLVVAAL"
     gene            complement(396..983)
                     /locus_tag="VFD06_10590"
     CDS             complement(396..983)
                     /locus_tag="VFD06_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN04019.1"
                     /translation="MQPDDFALMEAVREGREGAFETFVQRYQRRLYRLAWGYLRQHED
                     ALDAVQETMVKIYLARAHYRPESHPFTWASRILVNHCLDLLRRRRVRPADSLEEAQEH
                     RPGRELAPADPADSPQRRHERSEFRRRVEEAVATLPESQREIFVLRHFEEMSLQEIAR
                     ARGCALGTVKSSLHRAAAAVRDRMQRSTLPGESPC"
     gene            complement(1055..1270)
                     /locus_tag="VFD06_10595"
     CDS             complement(1055..1270)
                     /locus_tag="VFD06_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04020.1"
                     /translation="MTVFVVVAGVLTVLLVAYTLSQRRPPIPADADHAGVGDPARCLA
                     CHGPGQREARGPNHPLNDQCFGCHDRS"
     gene            complement(1300..1707)
                     /locus_tag="VFD06_10600"
     CDS             complement(1300..1707)
                     /locus_tag="VFD06_10600"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZN04021.1"
                     /translation="MSAIRDLPDLLAPDPIPGLTVLEEGLTFVGDIVSEESIRVRGRI
                     EGNIATSGSVVIEPRGTVLGEVTAENLILEGALEGRVTVARKFELRPTGRMRGDIRAA
                     VVAIAEEAFLQGKVLATERVTTTPQQARRAERH"
     gene            complement(1822..2292)
                     /locus_tag="VFD06_10605"
     CDS             complement(1822..2292)
                     /locus_tag="VFD06_10605"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HZN04022.1"
                     /translation="MPNLWNRRTREGEHGFTLVELLIVVAIVGLISAIAIPNLLNAVD
                     KAKQKRSMADMRTIGAAVEAYATDNARYPIGVTTWPAMKSHLNPHFMKEPPDTDGWQN
                     GWEVGSVSGDEYTITSTGKDGAASTRSGGPTQVFDCDILFTNGQFFQWPQGTQS"
     gene            2539..2610
                     /locus_tag="VFD06_10610"
     tRNA            2539..2610
                     /locus_tag="VFD06_10610"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2571..2573,aa:Thr,seq:cgt)
     gene            2665..4599
                     /locus_tag="VFD06_10615"
     CDS             2665..4599
                     /locus_tag="VFD06_10615"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HZN04023.1"
                     /translation="MSLRDTPFLEALSRGPLVFDGAMGTQLYERGYFITRSFDEANVA
                     RPDLVLDIHRDYVAAGAQILETNTFGANAECLKRYGAQDKVRAINRAGVRLAREAAGE
                     AAWVAGSIGPTGLMVGTLSEERRASLRSLFLEQITALLEEGVDLLVLETFYSLEEIEQ
                     ALLAARSVHHGPIVAQVAFSEERKLVDGLPPLEVARRLRDGGADVVGANCAEGPAYLF
                     EVGRAMVPGGAPVSVQPNAGRPRRLDERTLYMTTPEYFGVYARRMFQAGVRLVGGCCG
                     TGPEHIRPVVDAARMLGGGRIEAPAVPKGPAAAEVRRVEGIPMPEKSPLAAKIDRVWH
                     DRLADPRSRRPPRGPDDFVVSVEVNPEPGLDPKSALRAAALLKRAGADVINIADGPRA
                     VARMSNLALAMKVKQEVGLDVLLHVCCRDRNLLGLHSDLLGAWVLGIRNLVLITGDPP
                     KMGDYPAATAVFDLDSVGLLTLVDALNHGIDPAGRSLQDRTGFLLACGAEPAAHDYDH
                     ELRRLEEKRRAGAEVVMTQPVYDPAVVRRFLDDTRGMGMPILVGLCPLASHRNAEFLH
                     NEVPGMQIPIDIRQRMAAAPTPDAARRQGMLIAREMLDEIKSDVVGAYLMPQFGRFRT
                     AVEVLEVVGYDFAPEDRDPA"
     gene            complement(4563..7607)
                     /locus_tag="VFD06_10620"
     CDS             complement(4563..7607)
                     /locus_tag="VFD06_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HZN04024.1"
                     /translation="MGFLDRFRPPGGLAHPEADERLHAVERLATADLGLLEPVARGDA
                     DARVRRAATRRLRDPRVLAEVAKADADPAVREAAAASLLARALGARDEGDGLPALEGL
                     EDPRQLTAVARSAALESVAGRALERLADERGLAGVARHGEHAAVRLAAVARIESPTEL
                     SHIALRGEHRDTCLAALERIDDPELLETIALRARLKPIARKARGRLRGPDGEDGAAET
                     ERPQSDRAAQEHLIAAVDALGEDGEWEDVPSRLQALRDAWIEDLAAVDDDLDERFRER
                     CHAARQRLLGMQEERAERERRERDLEERLGPRRRLCEAVEGATSDSVAAVLQEARAAW
                     ASLPPAEGPEAEGLDARFLAACAAIESGQAQREAEAKARTERDEAARQSEAALQKRRE
                     SLKVLEKMAATCEALAKKEDASLAKLERALRDLRTALQEIPPLPTPKDHETIVRRLKG
                     LQADLTPRLQTLRDTDRWQRWANAAVQEELCARMEDLASAVEAEGADLSAAGSGMREL
                     MERWRAAGPAPPDRSLSLWNRFKAARDRVREKADDHFQKQIEEAGVNLKTKEALCEKA
                     EVLAVSNDWIKTAEAVKALQLEWKGIGPVSRGHEKAIWERFRKACDQFFKARDEDLAK
                     RKGEWAKHLESKVALCEKAEALAGSTDWKATAEEFKRLQAEWKTTGPVRRNQSEVIWN
                     RFRTACDRFFERFKNRDAVERDALVKERLALCAEIEALAPAPETPALSVETVPSAADA
                     VPDPAAETSAALDGTPDAGAATEAQATGLASPEAAAAPAEEPVAPPPAPPASPEEMVG
                     RLRAGLERWRRMRGLPPDQMGPLNTRFFAAFDRALKAHPQAFHGTSLDAEANLKRAQD
                     LVAKVQRLAPSSNGPDTSSLTPGERLASMWREALATNTMGGRIADESRARAAAEEARK
                     AQAAWQRLGYVPEPERRTLQADFDTACRAIFAARGADGPTGPEGRDRGRGGDGRPRGG
                     PGRGDGRPRGGGPGGGGPGGGGRPQGRPRDLQAGSRSSGAKS"
     gene            7844..>8272
                     /locus_tag="VFD06_10625"
     CDS             7844..>8272
                     /locus_tag="VFD06_10625"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN04025.1"
                     /translation="MGRIGRYEIESILGKGAMGVVFLARDPQLGRQVALKTYALPEGI
                     SDEVVREFEERLLREAHAAAALSHPNIVTVHDAGIDAERRFPYIVMEYVPGRSLKDFL
                     DGRHRLAPDLALRFGDALAWALDAAHSKGIVHRDIKPANIL"
BASE COUNT         1054 a         3119 c         2912 g         1187 t
ORIGIN      
        1 gcagcgcggc cacgacgagg agcgaagcgg ccgccgcggc cacccccgcc ggccacatcc
       61 atggcgatct cctgcggccg ggtgtctccg gggcggcggc cgccggctcg gcggcccggg
      121 cccgcgccac ggtgcggcgc gcgaagtcgt cccagtccac ctcccgctcg cgtgggaagg
      181 cggcacccga atcgaccgcg gcgagcgccc ggcgcgtctc ctcccacgcg gcggcgcact
      241 ccccgcaggc ggagaggtgc gcctcgacgc gcaggcggtc ggccgtctcc agaccggaat
      301 agaagtgcag cggcaggagc ggcaggatgg ccgcgcagcc ggacgactcg atgcggccgg
      361 tcacgcggcc cctccccggc gcggacgtcc aaggctcagc acggactctc tcccggcagc
      421 gtgctgcgct gcatgcggtc ccggaccgcc gcggcggcgc ggtgcagcga cgacttcacc
      481 gttccgaggg cgcagccgcg cgcgcgcgcg atttcctgga gcgacatctc ctcgaagtgc
      541 cgcagcacga agatctcccg ctggctctcg ggcagcgtcg cgaccgcctc ctcgacgcgg
      601 cggcggaact cgctgcgctc gtggcgtctc tggggcgagt cggccggatc cgccggcgcg
      661 agctcgcgcc ccggccggtg ctcctgcgcc tcctcgagcg aatcggccgg ccgcacccgg
      721 cgccggcgca ggagatcgag gcagtggttg acgaggatgc gcgacgccca ggtgaacggg
      781 tgcgactccg gccggtagtg cgcccgcgcc aggtagatct tcaccatcgt ctcctgcacg
      841 gcgtcgagcg cgtcctcgtg ctggcgcagg tagccccacg ccagtcgata gagacgcctc
      901 tggtagcgct ggacgaatgt ctcaaaggcc ccctcccgac cctcccggac cgcctccatg
      961 agggcgaaat cgtccggctg cactccgctt cctcccgcca tcacgcaaac gcaccggccc
     1021 caccgcgggt ttaccccggg cggctcctat gccctcagct cctgtcgtga catccgaagc
     1081 attgatcgtt gaggggatgg ttcgggcccc tcgcctcccg ctgtcccgga ccgtggcacg
     1141 ccaggcagcg cgccggatcc ccgaccccgg cgtggtccgc gtccgccggg atgggtgggc
     1201 ggcgctgcga cagcgtgtac gccacgagga ggaccgtcag gactcccgcc acgacgacga
     1261 agaccgtcac gcgggcggcg ccgcgggccc gctcgcgcct cagtggcgct ccgcccgccg
     1321 tgcctgctgc ggggtggtgg tgacccgctc ggtcgccagc accttcccct gcaggaacgc
     1381 ctcctcggcg atcgccacca cggcggcgcg gatatcgccg cgcatccgcc cggtcgggcg
     1441 cagctcgaac ttacgggcca cggtgacccg cccctcgagc gccccctcca ggatcaggtt
     1501 ctcggcggtg acctctccca gcacggtgcc gcgcggctcg atcacgaccg atccggaggt
     1561 ggcgatgttc ccttcgatgc ggccacggac ccggatgctc tcctccgaga cgatatcgcc
     1621 gacgaaggtg agcccttcct cgagcaccgt gagtcccggg atcgggtccg gggcgaggag
     1681 atccgggagg tcgcggatgg cgctcatcgg gtggcgcgcg gccggggtct gcgggcgcgg
     1741 gcggccccgc cggcgggcgg atgatagcaa gggtggcggg cgccatcaac cggcgcgggg
     1801 acgccccggg tcagccggac gtcagctctg ggtcccctgc ggccactgga agaactggcc
     1861 gttggtgaac aggatgtcgc agtcgaacac ctgcgtcggg ccgccggagc gcgtcgaagc
     1921 ggcgccgtcc ttgccggtgc tggtgatggt gtactcgtcc cccgacacgc tgccgacttc
     1981 ccagccgttc tgccagccgt cggtgtccgg aggctccttc atgaagtgcg ggttgaggtg
     2041 tgacttcatc gccggccacg tggtcacgcc gatcgggtag cgggcgttgt cggtggcgta
     2101 ggcctcgacc gcggcgccga tggtgcgcat gtcggccatg ctccgcttct gcttggcctt
     2161 gtccacggcg ttcaggaggt tgggaatcgc aatcgccgag atgagcccga caatggcgac
     2221 gacgatgagg agttcgacca gcgtgaagcc gtgttcacct tcgcgtgtgc ggcggttcca
     2281 caggttcggc atgatcgagg gctccggtcc cgcttccgca atccggatgc cacgcctccg
     2341 gccgatcccc ggcagccgca acggacgcgc ttcgaagggg ttgcgcgatg cgccgtccgg
     2401 gaatcacgaa accgtactgt cattctgtgt cgcttttcga cggtgaggct gccgcgcgac
     2461 gtcaggcgcg tggcgcttcg gccgaccgga tcctccttgc gctcggctcc ggcccccgct
     2521 tagactacgg ggctccgggc cgacgtagct cagtggtaga gcaactgatt cgtaatcagt
     2581 aggtcgccgg ttcaaccccg gccgtcggct ccgcccgcgt tccccgatgc ggccccggcc
     2641 gcgcgaggcc gtcccttcaa accgatgtcc ctccgtgaca ccccgttcct cgaagcgctg
     2701 tcgcgcggtc cgctggtctt cgacggcgcc atggggacgc agctgtacga gcggggctac
     2761 ttcatcaccc gctcgttcga cgaagcgaac gtcgcccggc ccgacctcgt cctcgacatc
     2821 caccgcgact acgtcgcggc gggggcgcag atcctcgaga ccaacacgtt cggcgccaac
     2881 gccgagtgcc tgaagcgcta cggagcgcag gacaaggtcc gggcgatcaa ccgggcgggg
     2941 gtgcgcctgg cgcgcgaggc ggcgggcgag gcggcgtggg tcgcgggatc gatcggaccg
     3001 accggcctga tggtcgggac cctgtcggag gagcggcgcg cgtcgctccg gagccttttc
     3061 ctcgagcaaa tcacggcgct cctcgaggag ggggtcgatc tcctggtcct cgaaaccttc
     3121 tacagcctgg aggagatcga gcaggcgctt ctcgcggcgc gctccgtgca ccacgggccg
     3181 atcgtcgcgc aggtcgcctt cagcgaggag cggaagctcg tggacgggct gccgccgctc
     3241 gaggtggcgc gccggctgcg cgacggcggg gcggacgtgg tcggcgccaa ctgcgccgag
     3301 gggccggcct acctcttcga ggtcggccgg gcgatggtgc ccggcggcgc gccggtgagc
     3361 gtgcagccga acgccggccg gccgcgacgc ctcgacgagc gcaccctcta catgacgacg
     3421 ccggagtact tcggcgtcta cgcgcggcgc atgttccagg cgggggtccg cctcgtcggc
     3481 ggctgctgcg gcaccggtcc ggagcacatc cggccggtgg tggacgcggc gcgcatgctc
     3541 ggcggcggac gcatcgaggc ccccgccgtc ccgaagggac cggccgccgc cgaagtgcgc
     3601 cgcgtggagg ggatcccgat gcccgagaaa tcccctctcg ccgcgaagat cgaccgcgtc
     3661 tggcacgacc gcctcgccga cccccggtcg cgccgcccgc cgcgcggccc ggacgatttc
     3721 gtcgtcagcg tcgaggtcaa cccggagccg gggctcgatc cgaaaagcgc cctgcgcgcc
     3781 gcggcccttc tgaagcgcgc gggggccgac gtcatcaaca tcgccgacgg gccgcgcgcc
     3841 gtggcgcgca tgagcaacct ggccctcgcc atgaaggtga agcaggaggt cggcctcgac
     3901 gtcctcctgc atgtctgctg ccgcgatcgg aatctgctcg ggctccactc cgatctcctg
     3961 ggggcgtggg tcctcgggat tcgcaacctc gtcctcatca ccggggatcc gccgaagatg
     4021 ggggactacc cggcggcgac cgccgtcttc gatctcgact cggtcgggct gctgacgctc
     4081 gtggacgcgc tgaaccacgg gatcgacccg gccggccggt cgctccagga tcgcaccggc
     4141 ttcctgctcg cctgcggcgc cgagccggcg gcgcacgact acgatcacga gctccgaagg
     4201 ctggaggaga agcggcgcgc cggcgcggag gtcgtgatga cgcagccggt ctacgacccg
     4261 gcggtggtgc ggcgcttcct cgacgacacg cgcgggatgg gaatgccgat cctcgtcggg
     4321 ctctgcccgc tcgccagcca ccgcaacgcc gagttcctgc acaacgaggt ccccgggatg
     4381 cagatcccga tcgacatccg gcagcgcatg gcggcggcgc cgaccccgga cgcggcgcga
     4441 cgccagggga tgctgatcgc ccgggagatg ctcgacgaga tcaagagcga cgtggtgggg
     4501 gcgtatctga tgccgcagtt cgggcgcttc cggaccgcgg tcgaagttct ggaagtggtc
     4561 ggctacgact tcgcgccgga ggatcgcgac ccggcctgaa gatcccgcgg acgcccctgc
     4621 gggcgaccgc cgccccccgg accaccgcct cccggaccac cgccgcgcgg ccgcccatcg
     4681 ccgcgccccg gaccgccgcg cggccgaccg tcaccgcccc ggccccgatc gcgcccctcg
     4741 gggccggtgg gaccatcggc gccgcgcgcc gcgaaaatgg ctcggcatgc cgtgtcgaag
     4801 tcggcctgaa gcgtgcggcg ctcgggctcc ggcacgtacc cgaggcgctg ccaggcggcc
     4861 tgcgccttgc gcgcctcctc ggcggcggcg cgggcacgcg actcgtcggc gatccttccc
     4921 cccatcgtgt tcgtcgcgag cgcctcgcgc cacatcgacg cgagccgctc ccccggcgtc
     4981 aacgaggagg tgtccggtcc gttcgaagag ggcgccagcc gctgcacctt ggcgacgagg
     5041 tcctgcgccc gcttgaggtt cgcctcggcg tcgaggctcg tcccgtggaa cgcctggggg
     5101 tgcgccttga gggcccggtc gaaggctgcg aagaagcggg tgttgagcgg gcccatctgg
     5161 tccggcggca ggccgcgcat ccgccgccag cgctcgagac cggcccgcag ccgtcccacc
     5221 atctcctcgg gggaggcggg cggcgcgggc ggcggagcga caggctcttc cgcgggggcc
     5281 gcggcggcct cgggcgacgc caacccggtg gcctgcgcct cggtcgcggc gccggcgtcc
     5341 ggcgtcccgt ccagtgcggc ggacgtttca gccgcggggt ccgggacggc gtccgcggcg
     5401 gacggcaccg tctccacgga gagggcgggg gtctccggcg cgggggcgag cgcctcgatc
     5461 tccgcgcaca gcgccagacg ctccttcacg agcgcgtccc gctcgacggc atcgcggttc
     5521 ttgaaacgct cgaagaagcg atcgcaggcg gtgcggaaac ggttccagat cacctcggac
     5581 tggttgcgcc gcaccggccc ggtcgtcttc cattcagcct gaagcctctt gaactcctcc
     5641 gccgtcgcct tccagtcggt cgagccggcc agcgcctccg ctttctcgca gagcgccacc
     5701 ttgctctcga ggtgcttcgc ccattccccc ttgcgcttcg ccaggtcctc gtcgcgcgcc
     5761 ttgaagaact gatcgcacgc cttgcggaag cgctcccaga tcgctttctc gtggccgcgc
     5821 gacacggggc cgatcccctt ccactcgagc tgcagcgcct tgaccgcctc ggcggtcttg
     5881 atccagtcgt tggagacggc gagcacttcg gccttctcgc acagcgcctc cttcgtcttg
     5941 agattgaccc cggcctcctc gatctgcttc tggaagtggt cgtcggcctt ctcacgcacc
     6001 cggtcgcgcg ccgccttgaa gcggttccac agcgacagcg accggtcggg cggggcgggt
     6061 ccggcagcgc gccagcgctc catcagctct ctcatccccg atccggcggc gctgagatca
     6121 gccccttccg cctcgacggc ggaggcgagg tcctccatgc gggcgcacag ctcctcctgc
     6181 accgccgcgt tggcccagcg ctgccagcgg tcggtgtcgc gcagcgtctg cagccgcggc
     6241 gtcaggtccg cctgcagccc cttcagacgg cggacgatgg tctcgtggtc cttcggcgtc
     6301 gggagcggcg ggatctcctg gagcgcggtc cgcagatcgc gcagcgcccg ctcgagcttc
     6361 gcgagggacg catcttcctt cttggcgagc gcctcgcacg tcgccgccat cttctccagg
     6421 actttcaggc tctcacggcg cttctgcagg gccgcttcgc tctgtcgcgc cgcctcgtcc
     6481 cgctccgtcc gggctttcgc ctcggcctcc cgctgcgcct gtcccgactc gatggcggcg
     6541 caggccgcga ggaaacgcgc gtcgagcccc tccgcctccg gtccttcggc cggcggcagg
     6601 gacgcccacg ccgcgcgggc ctcctgcagg accgcggcca ccgagtcgga cgtcgcccct
     6661 tcgaccgcct cgcacaggcg ccggcgcggg ccgagccgct cctccaggtc ccgctcgcgc
     6721 cgctcgcgct ccgcccgctc ctcctgcatg ccgagaagcc gctggcgcgc cgcgtggcag
     6781 cgctcccgga agcgctcgtc cagatcgtcg tcgaccgcgg cgaggtcttc gatccaagcg
     6841 tcccgcagcg cctgcaggcg ggacggcacg tcctcccact cgccgtcctc tcccagcgca
     6901 tcgaccgcgg cgatcagatg ctcctgcgcg gcccggtcgc tctgcggcct ttcggtctcg
     6961 gccgcgccgt cctcgccgtc gggcccacgg agtcgcccgc gcgccttgcg ggcgatcggc
     7021 ttgaggcgcg cccgcagggc gatcgtctcg aggagctccg gatcgtcgat ccgctccagc
     7081 gcggcgaggc aggtgtcgcg gtgctcgccg cggagcgcga tgtgcgacag ctcggtgggg
     7141 ctttcgatgc gcgcgaccgc cgcgagcctc accgccgcgt gctcgccgtg ccgcgccacg
     7201 ccggccaggc cgcgctcgtc cgccagccgc tcgagcgccc gtcccgccac cgactcgagc
     7261 gcggccgatc gggccaccgc cgtcagctgc cgcggatcct cgaggccttc gagcgccggc
     7321 aggccgtccc cctcgtcacg cgcgccgagg gcgcgggcga ggagggacgc ggccgccgct
     7381 tcgcggaccg ccgggtcggc gtcggccttg gccacctcgg ccaggacgcg cggatcgcgc
     7441 aggcggcggg tggcggcacg ccgcacccgc gcgtcggcgt ccccgcgcgc caccggctcc
     7501 aaaagtccca ggtcggccgt cgccagacgc tcgacggcgt gcaatcgctc gtcggcttcc
     7561 gggtgcgcca gccctcccgg gggcctgaat cggtcgagaa aacccacgcg ttccctcggt
     7621 ctcagaaagg aaagtcggat catacaccgg ccccgccgca ggcaaaacat cggcttgcgg
     7681 gacgatgacg aatcgggaat cggggccccc gggcccccgc cggaagggtc ccccgcgccg
     7741 cggcttgaat cacccggcct cgggcgccag attggaccgg gtcgcccgcc tgccgccgtc
     7801 cggccgcccc ggaaggaccg cgcggcgcgg cggacgggag tccatgggcc gcatcgggcg
     7861 ctacgagatc gagtcgatcc tcggcaaggg tgccatgggg gtggtgttcc tggcgcgcga
     7921 cccgcagctc ggccgccagg tcgccctcaa gacctacgcc cttcccgagg ggatctccga
     7981 cgaggtggtg cgggagttcg aggagcgcct cctgcgcgag gcgcacgccg ccgccgcgct
     8041 gtcccacccc aacatcgtga cggtgcacga cgccggcatc gacgcggagc gccgcttccc
     8101 gtacatcgtc atggaatacg tcccgggaag gagcctcaag gactttctcg acggccgcca
     8161 ccgcctggcg ccggacctgg cgctgcgctt cggcgacgcg ctggcctggg cgctcgacgc
     8221 cgcgcacagc aaagggatcg tgcaccggga catcaagccg gccaacatcc tg
//
LOCUS       DASRIQ010000435         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_413458, whole genome shotgun sequence.
ACCESSION   DASRIQ010000435 DASRIQ010000000
VERSION     DASRIQ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413458"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /gene="amrB"
                     /locus_tag="VFD06_10630"
     CDS             complement(<1..246)
                     /gene="amrB"
                     /locus_tag="VFD06_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZN04026.1"
                     /translation="MAGMFYPAGEEPLRSELRALIRGGRPEAAAPLALIVPHAGYVYS
                     GRIAARTYLSGTLADRIVVLGPNHTGEGEAIAVQAEGA"
     gene            418..1287
                     /locus_tag="VFD06_10635"
     CDS             418..1287
                     /locus_tag="VFD06_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZN04027.1"
                     /translation="MRIALAQIAPPLGDPLGSLRLHLATARRAAKAGAALVVFPELSL
                     TGYRLQDLVPEVAMPLDPKGLLRPLFALSRSISIAFGMVEESEGHRYYNSAVFLERGK
                     VRHVHRKVYLPTYGMFEEGRYFAAGDAFTAFRSRLGRIGMLVCEDLWHPASALLLAQD
                     GADYLLVLANSPVQAGQGAGGPRNHETWRDLIRVTAQMQTVYVLLTNRAGCEDGISFS
                     GASAAVDPLGQLLAEGRMLGDDLVVCDLDRRALRRARTFYPLLRDERLGVLQREIGRL
                     LAAPEAARGPGGS"
     gene            1326..2396
                     /locus_tag="VFD06_10640"
                     /pseudo
     CDS             1326..2396
                     /locus_tag="VFD06_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            complement(2921..>3119)
                     /locus_tag="VFD06_10645"
     CDS             complement(2921..>3119)
                     /locus_tag="VFD06_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZN04028.1"
                     /translation="APAKQMIFDVDVLLAYISRVMTLEPGDVVATGTPEGVGPLAAGD
                     EVSVAIAGIGRLVNRVEADRD"
BASE COUNT          428 a         1158 c         1077 g          456 t
ORIGIN      
        1 cgccccctcc gcctgcacgg cgatcgcctc cccttccccg gtgtggttcg gtcccagaac
       61 gacgatgcga tccgcgagcg ttccggaaag gtaggtccgg gccgcgatcc gtccggagta
      121 gacgtagccg gcgtgcggga cgatgagggc gagcggagcc gcggcctccg gccgccctcc
      181 gcggatgagc gcccgaagct cgctgcggag cggctcctct ccggccggat agaacatccc
      241 ggccacagcg ggcctcctga tcatcgccgt ccccacccgt cggcgcccca agaaaacgac
      301 tcgctgtcgg gttcctttcc gggccgcgcg atgctagcaa acgatcgctc caaagtaaag
      361 aaacagaatc acttgcgtgg atcgggtcgc ttcaggcgtg ctacaattcg cgactccatg
      421 cgcatcgcat tggcccaaat cgccccgcct ctcggggacc ctttgggcag cctccgactg
      481 cacctcgcga ccgcccgccg cgcggcgaag gcgggggcgg cgctcgtcgt cttcccggag
      541 ctcagcctca ccggctaccg cctccaggat ctggtgccgg aggtggcgat gccactcgac
      601 ccgaaggggc tcctgcgccc cctcttcgcg ctgagccgca gcatctcgat cgccttcggc
      661 atggtcgagg agtcggaggg gcaccgctac tacaacagcg ccgtcttcct cgagcggggg
      721 aaggtgcgcc acgtccaccg gaaggtgtac ctgccgacct acgggatgtt cgaggagggg
      781 cggtacttcg ccgccggcga cgccttcacg gcgttccgca gccggctggg ccgcatcggc
      841 atgctggtct gcgaggacct gtggcacccg gcgagcgccc tccttctggc gcaggacggg
      901 gccgactacc tgctcgtgct cgccaacagc ccggtgcagg ccggacaggg agcgggcggg
      961 ccgcgcaatc acgaaacctg gcgcgacctc attcgggtga cggcgcagat gcagaccgtc
     1021 tacgtgctcc tcaccaaccg ggccggctgc gaggacggca tctccttctc gggagcgtct
     1081 gcggccgtcg atccgctcgg ccagctgctg gcggaagggc ggatgctcgg cgacgacctc
     1141 gtcgtctgcg acctcgatcg ccgggcgctc cgccgcgccc gcaccttcta tccccttctg
     1201 cgcgacgagc gcctgggcgt gctgcagcgg gagatcgggc gtctcctcgc cgctcccgag
     1261 gcggcccgcg gaccgggggg atcgtgaaga tccatctcgt ctacaagtgc cacgacctcg
     1321 ggcggatgga gttctactcg cgcttcacgc cgctcgggct cgggacgatg acggcgcacc
     1381 tgcgccggga agggttcgac gcgacgctcg tcaacctgag cgcctggtcg tggccgaagg
     1441 cggcgcggtt cttcgccgcg gagcgccccg acatcgtcgg ggtgtcggcc ttcaccttca
     1501 accggcacga ggcgatgcgg gtcgccgccc tcgcgcgcgc cgccaacccg cgctgtctcg
     1561 tcctcgccgg cggcccgcac gccacgcaca tgccgcacca tctcctggat cactacccgc
     1621 agatcgatct ggtggtgcgc ggcgaggggg aggagacgat gctggcgatc gcccgcgccg
     1681 ccgcggccgg gacgatcgtc cggccggacc tcggcatggc cccggcgatt ggacgcgacc
     1741 tcgaagggac cggtccccag ggcgggccgc acgcgatcgc cggcctcacc taccgcgcgt
     1801 cggacggctg ccgcgacacc ctcgaccggc ctcccgtcaa agacctcgac acgctccccc
     1861 accccgccgc cgacccccac ctggtcggcg tcgaggcgga gtcgcagttc gagttcgtca
     1921 tcacctcgcg cggctgcccg gccgcctgca ccttctgctc gtcgccggag ttctgggggc
     1981 gcggcctccg gttccgctcg gccgcccaca tgctggagga ggtacggctg ctgcgcgaga
     2041 agcacggggt cgtctacctc tccatccggg acgacacctt cacggcccac cggcagagag
     2101 tgctcgactt ctgccgcatg ctcgaggaat cggggatcga tctcctctgg gactgtcagt
     2161 cgcgggtcaa catcgtcgac gaggagcggc tggtcgccat gcgccgcgcc ggctgcaccc
     2221 acatccagta cggcgtcgag tccggctctc cccggatgct gcagcgcctg aacaagggga
     2281 tcggcgtgga cgagatccgc cgtgccgcgg cgctgacccg caaggtcggc ctcggcctgt
     2341 cgatctacct catcaccggg atcgacggcg agaccgacga ggatctcgcc tcgaccgtgc
     2401 gcctcatcga ggagatccgg ccccacgacg ggctggtctc gccgctgacc gtctacccgg
     2461 ggaccggact gcaccaggag gcgaagcggc gtctcgggct cggcgacgac ttctggatcc
     2521 gcagccgcga ggaggcgttc ttcgtgcgcc gcgatccctg gacgcggcgc gccgtgcgca
     2581 ccctccgggc gacgctccgg cgcgtcgggc gggcggcggc gtacggtcag gaggacttcg
     2641 agcggcagcg cggcctcgtc ggcgattgcg cgacgctccg gctcgccgtc ggggagtcct
     2701 gggagcggcg cggaaggctg ctgcgggctc gcgaggagta cgcggcgctc ctcgccgcgc
     2761 agccgcgcag cctctgggcc cgcatgcggc tggggcacat cgcggcgcgc cggcgccgct
     2821 acgacgaagc ggcggagcac tacgaggcgg ccacgcggat cgcgccggct ttccgccacg
     2881 cggaccagct gctccgcgag gcgcgccgga acctccgcgg tcagtcccgg tcggcttcga
     2941 ctcggttgac caggcggccg atgccggcga tcgcgacgct cacctcgtcc ccggcggcga
     3001 gcgggccgac cccttccggc gtgccggtcg ccaccacgtc tcccggctcg agggtcatga
     3061 cccgggagat gtaggcgagc aggacatcga cgtcgaagat catctgcttc gccggcgcc
//
LOCUS       DASRIQ010000436         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2503338, whole genome shotgun sequence.
ACCESSION   DASRIQ010000436 DASRIQ010000000
VERSION     DASRIQ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503338"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFD06_10650"
     CDS             <1..374
                     /locus_tag="VFD06_10650"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HZN04029.1"
                     /translation="LRQVTRDVRPLLMAVHSFTPIYEGRRRDFDMGVLFDRSRGHAAL
                     FLRGLRREGFRVRANEPYSGLAGMMYSIERHGTHHDLPCLEIEMNQELFRDRAAASRL
                     APRIARALRPVLALQRRPTRR"
     gene            complement(394..>2349)
                     /locus_tag="VFD06_10655"
     CDS             complement(394..>2349)
                     /locus_tag="VFD06_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04030.1"
                     /translation="AGVSFRAVSTALGESPFVPPDSMGDIGPTQILVHVNGRIRVFDR
                     NGVIGPLNSNNNNFWEAVRNGFRLSDPNVRYDRLSGRWFLVMMNLETVNNRIVLAVSD
                     GPTITGSTGFTLFYFNVGIAPAEAANVCDYPGVGVDAHAVTIGCNMYSSAGSFQHSTV
                     YVVRKSSLLSGGPIVVTPFVNVTGGITAGPWSPRGVDNDDPLATESYFIGVDIPLLGG
                     LMLRRVTDPGGTPLLSGNIPLAIPATSLPLLQVSQDSSIGLDTLDDRLFMAAITMNKV
                     TGVASLWTAHHLAVDASCVAAVSGSGRRNAARFYEFGDLTAPTPTLLQAGTLCDTAAA
                     GPRGFIYPSVIGTGQGHVAIGASSASANAYAGVAVASRRRDDPPGTIGAPLFPQSGLA
                     GYTVVAGSFNRWGDYSFTGVDPNDDMTVWTFQEYADAPASNWAVRVLQLVAPPPAVPA
                     AASPAAICAGAAAAQVTITGDGSSGGEFFDPGPDTGGPGFASHLAAAACPGVTVTSAA
                     FIDPTHVALTLDAGAAVPGGCDIALTNPDGQSATGPGLLAILAPPVEVNDTLALSDTG
                     GLTSLAWNDPPGEFNVYRGVRSGASWTYNHTCFAPHLASQGTTDPEAPPVGSVFFYLV
                     ARTNDCGESAPGRDSQGAPNPIPAPCN"
BASE COUNT          349 a          791 c          865 g          344 t
ORIGIN      
        1 tcctccggca ggtgacccgc gacgtccggc ccctgttgat ggcggtgcac tcgttcacgc
       61 cgatctacga agggcggcgg cgggatttcg acatgggggt gctcttcgat cgcagtcgcg
      121 gccacgcggc gcttttcctg cgcgggctgc ggcgcgaggg gttccgggtg cgggccaacg
      181 agccgtactc ggggctcgcc gggatgatgt actcgatcga gcgccacggc acgcaccacg
      241 atctcccctg tctcgagatc gagatgaacc aggagttgtt ccgggatcgc gccgcggcgt
      301 cgcgcctggc gccgcgcatc gcccgggcgt tgcggccggt gctggcgctg cagcggcgcc
      361 cgacgcggcg ttgacccgcc ctgtggaccg ttttcagttg cagggggccg ggatcgggtt
      421 cggagccccc tgcgaatcgc ggccgggtgc cgactccccg cagtcattcg tgcgcgccac
      481 gaggtagaag aagaccgagc cgacgggcgg cgcctcgggg tcggtcgtcc cctgcgacgc
      541 gaggtgcggg gcgaaacagg tgtggttgta ggtccacgag gctcccgagc gcaccccgcg
      601 gtagacgttg aactcccccg gaggatcgtt ccaggcgagg ctggtgagcc cgccggtgtc
      661 ggacagggcc agcgtgtcgt tcacctccac ggggggggcc agaatcgcca ggagcccggg
      721 gccggtggcg gactggccgt ccgggttggt gagggcgatg tcgcaccctc ccggcaccgc
      781 ggcgcccgcg tcgagcgtca gcgcgacgtg cgtcggatcg atgaaggcgg cgctcgtaac
      841 cgtgacgccg ggacaggcgg cagccgccag gtgcgacgcg aaacccggcc ctccggtgtc
      901 aggtccggga tcgaagaact ccccgccgga tgagccgtcc ccggtgatcg tcacctgcgc
      961 cgcggccgcc cccgcgcaga tcgcggcggg tgacgccgcc gccggcaccg ccggaggcgg
     1021 agcgaccagc tgcagcaccc gcacggccca attgctggcg ggcgcgtccg cgtattcctg
     1081 gaaagtccac acggtcatgt cgtcgttcgg atcgaccccg gtgaaggagt agtcccccca
     1141 ccggttgaag ctgccggcga ccaccgtgta tccggcgagc ccggactgcg ggaagagcgg
     1201 cgcgccgatc gtcccgggcg ggtcgtcccg ccgccggctc gccaccgcta cccccgcata
     1261 cgcgttcgcc gaggcggacg acgcaccgat cgcgacgtgc ccctgcccgg tcccgatgac
     1321 gctcggatag atgaagccgc gcgggccggc cgccgccgtg tcgcacaagg tgcccgcctg
     1381 cagcagcgtc ggcgtcggtg cggtcaggtc gccgaattcg tagaagcgtg cggcgttgcg
     1441 gcgcccgctg ccgctcaccg ccgcgacgca ggatgcgtcc accgcgagat ggtgcgccgt
     1501 ccagaggctg gcgacgccgg tgaccttgtt catcgtgatg gcggccatga acaggcggtc
     1561 gtcgagggtg tcgaggccga tcgacgagtc ctgcgacacc tgcaggagcg ggagcgacgt
     1621 cgcagggatc gccagcggaa tgttccccga gagtaagggg gtgccgcccg gatcagtgac
     1681 ccgtcggagc atcagcccgc ccaggagcgg aatgtccacg ccgatgaaat acgactcggt
     1741 cgcgagcgga tcgtcgttgt cgacgccgcg cggagaccag gggccggcgg tgattccccc
     1801 ggtcacgttg acgaacgggg tgaccacgat cgggccgccc gacagcaggc tcgacttgcg
     1861 gacgacgtag acggtggagt gctggaagga cccggcgctg ctgtacatgt tgcagccgat
     1921 cgtcaccgcg tgcgcgtcca cgcccacccc gggatagtcg cagacgttcg cggcctcggc
     1981 cggcgcgatg ccgacgttga aatagaagag cgtgaatccg gtcgatccgg tgatcgtcgg
     2041 cccgtcgctc acggcgagca cgatccggtt gttcaccgtc tcgaggttca tcatcaccag
     2101 gaaccagcgg ccggagagcc ggtcgtagcg cacgttggga tccgacaggc ggaacccgtt
     2161 ccgcaccgcc tcccagaaat tgttgttgtt cgagttgagc ggcccgatga cgccgttgcg
     2221 gtcgaacacc cggatgcgcc cgttgacgtg gaccaggatc tgggtcggac cgatgtcccc
     2281 catgctgtcg gggggcacga acggcgattc gccgagggcg gtgctgacgg cgcggaagga
     2341 gacgccggc
//
LOCUS       DASRIQ010000437         9302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_711353, whole genome shotgun sequence.
ACCESSION   DASRIQ010000437 DASRIQ010000000
VERSION     DASRIQ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711353"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1358
                     /locus_tag="VFD06_10660"
     CDS             87..1358
                     /locus_tag="VFD06_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN04031.1"
                     /translation="MKLPIDGPLDRVQQDAIAAEGIDNWNEYLRRFPKTKDRDRDRHL
                     VEWSGEGSLIRNIYGEEYIDCLGGYGIFSLGHRHPEVIAAVKSQMDRMPLHSQYLVNP
                     ITAEAARRLAEVAPGNLKRTFFCNSGTEAVEGALKVARLHTKRRQFVSTQNAFHGKSL
                     GALSVTGRQVFRAPFAPLLQDVAFVPYGDADLLDRAVGPDTAAVILEPVQGEGGVIVP
                     PADYLPRAREICTKKGALLIADEVQTGLGRIGALFGVNLTGVVPDVLCLAKALSGGVI
                     PCGSFTTTDAIYEAFHENPMYHSSTFGNNPLAATAAAMAIEITVRDRLPERSARLGTN
                     LLGRLRRLQEKHPQAIREVRGQGLLIGLEMRDERLGGALAEALYARRILVAYALNKPE
                     VVRIEPPLNIPEEYLDRLVEALGDSLTSLPA"
     gene            1423..2103
                     /locus_tag="VFD06_10665"
     CDS             1423..2103
                     /locus_tag="VFD06_10665"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HZN04032.1"
                     /translation="MTPSARTAPEPFRFRDCSLTIVSLGRSARNLRELRAELSAVPTG
                     SIQHHFYDALLRPSFDDREYRNDFAAWTRRMLRDAPLAERLSVIDPLDCADFEDLRDR
                     LIEVVEERLAEGVWHAEVPEGMEFRFLRSQLVFFDTGARASSIQELREMLPKTTTGSV
                     FYHFVNARFRSPERRDDYSTWLLGLGDEGKAIADRLRRIDFQLWSLSETRGRIAAVLS
                     GADGRVVA"
     gene            2100..3338
                     /locus_tag="VFD06_10670"
     CDS             2100..3338
                     /locus_tag="VFD06_10670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN04033.1"
                     /translation="MSIGCRDYAEAAGSEVIDALERLARPLRGRRVVHVNSTRVGGGV
                     AEILVRLVPLMKDLGLDAAWEVIEGTQEFYGVTKTFHNALQGVPAAPTERGLAVYDEV
                     NREAARVLGPSLQDAEFVFIHDPQPAAILRHLPDRRGHWVWRCHIDVSRPYRPVWKFL
                     RPIVAPYEASVYSMAAFAQPLPHPQYIIAPSIDPLSDKNRPLEESEVAAVAARHRLDP
                     ARPVVVQVSRFDRFKDPLGVLQAYRIARRNLPIQLVLVGGTATDDPEGDAVLAAVREA
                     AGDDPDVHVLLLPPDAHREINALQRRATLVMQKSLKEGFGLTVTEALWKGKPVIGGRA
                     GGIVLQVVDHHTGFLVDTPEGAAFRIRYLLNRPNAVATMAEKGRAFVRDNFLLTRHLR
                     EYLTLMLALDSGGAEQVEVP"
     gene            3335..4147
                     /gene="otsB"
                     /locus_tag="VFD06_10675"
     CDS             3335..4147
                     /gene="otsB"
                     /locus_tag="VFD06_10675"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HZN04034.1"
                     /translation="MSGREGRDPETHGEGAFVPESLWRRLTVAPHRLLAFDFDGTLAP
                     YARDPLDARALPEALERLARLAGRDATTIAVVSGRPIPNLERVVPIERLHFVGEHGWE
                     ERPPGADTIHHPLPQEAAEALARILDVVTREGTKAKVERKRTGVAVHTRALDRDERDR
                     ALAHFESVFRRAAEGAGLRLDVLDGGAEAHAAGRDKGTALRALLAALPSGTVPVYVGD
                     DVTDEPAFEAAQERGFGIKVGPAATRARHALDGPHAVAGLLAGLLRALEETA"
     gene            4144..5646
                     /locus_tag="VFD06_10680"
     CDS             4144..5646
                     /locus_tag="VFD06_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HZN04035.1"
                     /translation="MSRLIVVSNRLPAPPATGPHLVGRETTVGGLAAAILGALQGRAG
                     SLWVGWNGRTAASGRAAGTLLHHRREGVDLASFPLTSAELDGYYHGFCNQTLWPVLHC
                     FQGRVRTRLRDEAAYDAVQGRFADLLMPMLRRDDLVWVHDYHLIPLGRELRRRGWRGR
                     IGFFLHTPFPPLELWEVLPRPRGMLEALFEYDVVGFHVQGFLDNYVYCTRRLLGADWD
                     GRRLAQAGRAQIASVYPVGIDPDAWRPHAGHGPAAGDGGEDAAARVGEASVANLKVLR
                     GLQDRRMILGVDRLDYTKGIPQRLQAVERFFRRRPEWRRRAVYVQVASPSRVQIPEYA
                     EQRRRIEATLGRVNGELSEPDWTPIRYLYRAYDRPLLARLYREADVGLVTPLRDGMNL
                     VAKEYVAAQDPADPGVLILSHTAGAAQQLKDAVLVNAFVPADVAEGIAFALEMPLAER
                     QRRHGRLLATVVDSTAEVWADRFVNDLERAVAASPYQVAGPEFVPPSTQI"
     gene            5673..7196
                     /locus_tag="VFD06_10685"
     CDS             5673..7196
                     /locus_tag="VFD06_10685"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN04036.1"
                     /translation="MPRMWIGGRFTDSVSHRTFPVDNPANERILARVPRADDRDADRA
                     VDAAAEAFRRWRQVSGLERANLMHQFAARLRAKHVAIARQLTLEGGKPYIENLDEIEW
                     CAGVFDYYGEIARDQMGKVIAPVFPHQVNFVRKEPYGVVVCIVPWNYPLLLLSWKAAA
                     AIAAGNTLVVKPSEETPLSTLMLAEDFDLFGKGVVNIVTGFGPEVGEPLVTHRDVSLI
                     AFTGSIATGKKIAAAAAPHLKKLNLELGGNDPFIVCDDVDVAIAAKAAAWAAFLNMGQ
                     VCTSGERFYVFERVYEEFVERFIAIAKGLRLGDPMGPDIDCGPLIRENHRLQIERKIE
                     EAVAQGATIRCGGRRPAKLKKGFFFEPTVLTDVTPSMTLMKSETFGPIAPIVPVKGIE
                     EAVTLADDSEYGLGANIYTNNLEWAMFAMERIHAGTFWINDPLTDNDAGPFGGMRMSG
                     MGRELGIEGLDAFREPKHVHMDYRIEAKPYWYPYRWKGVPGAPAPRRKSKARPRARR"
     gene            7205..8605
                     /gene="gabT"
                     /locus_tag="VFD06_10690"
     CDS             7205..8605
                     /gene="gabT"
                     /locus_tag="VFD06_10690"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZN04037.1"
                     /translation="MKKHIVLKTPIPGPRSQQAFEARRAAVPRGAYNITPVAIAKGEG
                     AILEDLDGNRFIDFAGGIGVLNAGHAPPAVVKAVQQQAEKFLHSCFHITINEPYLRLA
                     EAMNRITPGAFPKKTLLVNSGAEAVENAVKIARGATRRPGIICFEDAFHGRTNMALSL
                     TSKTHPYKEHFGPFNSEVHRMPYAYCYRCPWGKKYPGCNMECAHFLTDFFKRYVEPDT
                     VAAILVEPILGEGGFVTPPPEYYPILTRLARERGILIIADEIQTGFGRTGRMWACEHY
                     GLEPDILVASKSIASGLPIAAVTGRAEIMDAPMEGSLGGTFGGNPVAAAAALATLEQF
                     EKQDLPGRARRIGDRIRARFEELWKKCPAVGNVRGLGAMMAIEFVKDRATKEPAKEMV
                     QTLVRLCYERGLITIPAGTYGNVFRALMPLVITDEQLDEGLDVLEAAILEAAQPIMAT
                     ATPAAAIPAGAPTPRT"
     gene            complement(8612..9082)
                     /locus_tag="VFD06_10695"
     CDS             complement(8612..9082)
                     /locus_tag="VFD06_10695"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN04038.1"
                     /translation="MSDPVQTFPTRRLYPPVYFLLALVVMLLLHFILPVRRLIPPPWT
                     WLGLLPFVVGGALTVRAAALFDRLGTPVKPFERSTRLVLEGPYRYTRNPMYLGMVVVL
                     SGVAIRLGTLSPFVVPPLFAHFIQTRFIVHEEAMMERLFGTEYLAFKTRVRRWL"
     gene            complement(9110..9302)
                     /locus_tag="VFD06_10700"
                     /pseudo
     CDS             complement(9110..9302)
                     /locus_tag="VFD06_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794057.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
BASE COUNT         1434 a         3288 c         3267 g         1313 t
ORIGIN      
        1 atccgcatcg aaccggcgtt gttcatcacc cgggagcagg tggaggaagt cctgaaccgc
       61 ctcgacgaca ccctgaagag cctgacgtga agctgccgat cgacgggccc ctggaccgtg
      121 tgcagcagga cgccatcgcc gccgagggga tcgacaactg gaacgaatac ctgcggcgct
      181 tcccgaagac caaggaccgc gatcgcgacc ggcacctcgt cgaatggagc ggcgagggat
      241 ccctcatccg gaatatctac ggcgaggaat acatcgactg tctcggcggc tacggcatct
      301 tctcgctcgg ccaccgccac ccggaggtca tcgccgcggt caagtcgcag atggaccgga
      361 tgccgctgca cagccagtac ctggtgaacc cgatcaccgc cgaggcggcg cgacggctgg
      421 cggaggtcgc ccccgggaat ctcaagcgca cgtttttctg caacagcggc accgaggcgg
      481 tggagggggc gctcaaggtg gcccgcctgc acacgaagcg ccggcagttc gtctccacgc
      541 agaacgcctt tcacggaaag tcgctcgggg cgctgtcggt gaccggccgc caggtgttcc
      601 gcgcgccgtt cgcgccgctc ctgcaggacg tcgcgttcgt gccgtacgga gacgcggacc
      661 tcctcgaccg ggcggtcggt cccgacacgg ccgccgtcat cctcgagccg gtgcaggggg
      721 aggggggcgt gatcgtcccg ccggccgact acctgccacg ggcgcgggag atctgcacga
      781 agaagggggc gttgctcatc gccgacgagg tgcagaccgg gctcgggcgg atcggcgccc
      841 tgttcggtgt gaacctgacc ggtgtcgtgc cggacgtcct gtgcctcgcc aaggcgcttt
      901 cggggggcgt catcccgtgc ggatcgttca cgaccaccga cgcgatctac gaggcgttcc
      961 acgagaaccc gatgtaccac tcgtccacgt tcggcaacaa tccgctcgcc gccaccgccg
     1021 ccgccatggc gatcgagatc accgtgcgcg accggctgcc ggagcgctcg gcgcgcctcg
     1081 gcaccaatct cctgggccgc ctgcgcaggc tccaggagaa gcacccacag gcgatccgcg
     1141 aggtgcgcgg tcaaggcctg ctgatcggcc tggagatgcg cgacgagcgg ttgggggggg
     1201 cgctcgcgga agcgctctac gcgcggcgca tcctcgtcgc gtacgcgctc aacaagccgg
     1261 aggtggtgcg catcgagccg ccgctcaaca tccccgagga gtatctcgac cgcctggtcg
     1321 aggcgctcgg ggactcgctg acgtcccttc cggcctgaag gcggccatca tgaaagcgcg
     1381 cttccgactt gccggcgcgg aaagcgtcgt tcagaatcaa ctgtgactcc gagcgcccga
     1441 accgctcccg agccgttccg tttccgcgac tgctcgctga ccatcgtctc gctcggccgg
     1501 agcgcccgca atctccggga gctgcgcgcc gagctctcgg cggtgccgac cggcagcatc
     1561 cagcaccatt tctacgacgc gctgctgcgt ccgtcgttcg acgatcgcga gtaccgcaac
     1621 gacttcgccg cctggacgag gcggatgctg cgggacgcgc cgctggccga gcggctgtcg
     1681 gtcatcgatc cgctcgactg cgccgacttc gaggacctgc gcgaccgcct catcgaggtg
     1741 gtggaggagc ggctcgccga gggggtctgg cacgccgagg tgccggaggg gatggagttc
     1801 cgcttcctgc gctcgcagct tgtcttcttc gacaccgggg cgcgcgcctc ctccatccag
     1861 gagctgcgcg agatgctgcc gaagacgacg accggcagcg tcttctacca cttcgtcaac
     1921 gcccgcttcc gctccccgga gcggcgcgac gactactcca cgtggctgct cggcctgggc
     1981 gacgaaggga aggcgatcgc cgatcgcctg cggcgcatcg acttccagct ctggtcgctc
     2041 tcggagaccc gcggccgcat cgcggcggtc ctctccgggg cggacggcag ggtggtggca
     2101 tgagcatcgg ttgcagagac tacgccgagg cggccggctc cgaggtcatc gacgccctgg
     2161 agcgcctggc gcggccgctg cgcggccgcc gggtggtgca cgtgaactcc acgcgtgtcg
     2221 gcggcggggt cgccgagatc ctggtgcgcc tcgtgccgct gatgaaggac ctcggcctgg
     2281 acgcggcctg ggaggtcatc gaggggaccc aggagttcta cggcgtaacc aagaccttcc
     2341 acaacgcgct gcagggggtc ccggccgccc cgaccgagcg cgggctcgcg gtgtacgacg
     2401 aagtgaaccg cgaggccgcc cgcgttctcg ggccgtcgct gcaggacgcc gagttcgtct
     2461 tcatccacga cccgcagccg gcggcgatcc tgcgccacct gccggaccgg cgcggccact
     2521 gggtgtggcg ctgccacatc gacgtcagcc ggccgtaccg gccggtctgg aagttcctcc
     2581 ggccgatcgt cgccccctac gaggccagcg tctactccat ggcggcgttc gcccagccgc
     2641 tgccgcaccc gcagtacatc atcgcgccga gcatcgatcc actcagcgac aagaaccgcc
     2701 ccctcgagga gtccgaggtg gcggccgtcg ccgcgcgcca ccggctcgac ccggcccggc
     2761 cggtcgtcgt gcaggtctcc cggttcgacc gcttcaagga tcccctgggc gtcctgcagg
     2821 cgtatcgcat cgcccgccgc aacctgccga tccagctcgt gctcgtcggc ggcaccgcca
     2881 ccgacgaccc ggaaggggac gcggtgctgg cggcggtgcg cgaggcggcc ggcgacgacc
     2941 cggacgtgca cgtgctcctg ctcccccccg acgcgcaccg cgagatcaat gccctgcagc
     3001 gccgcgccac gctggtgatg cagaagtcgc tcaaggaggg gttcggtctc acggtcaccg
     3061 aggcgctgtg gaaggggaag ccggtgatcg gcgggcgggc cggcggcatc gtcctgcagg
     3121 tcgtcgatca ccacaccggc ttcctcgtcg acactccgga gggggcggcg ttccgcattc
     3181 gctatctcct caaccgcccg aatgccgtcg ccacgatggc ggagaagggg cgggccttcg
     3241 tgcgcgacaa cttcctcctg acgcgccacc tgcgcgagta cctgacgctg atgctggcgc
     3301 tcgattccgg gggcgcggaa caggtggagg tgccgtgagc ggccgggaag gccgcgaccc
     3361 tgaaacgcac ggtgaggggg ccttcgtccc ggagtcgctg tggcgccgcc tgacggtggc
     3421 gccgcaccgg ctgctggcct tcgacttcga cggaacgctc gccccctacg cccgcgaccc
     3481 gctcgacgcg cgggcgctgc cggaggcgct cgagcggctg gcgcgcctcg ccggtcgcga
     3541 cgccacgacc atcgccgtgg tctccgggcg tccgatcccg aacctcgagc gcgtcgtgcc
     3601 gatcgagcgg ctgcacttcg tcggcgagca cggctgggag gagcggccgc cgggcgccga
     3661 cacgatccac cacccgctgc cgcaggaggc cgcggaggcg ctcgcgcgca tcctcgacgt
     3721 ggtgacgcgc gaggggacga aggcgaaggt ggagcgcaag cggaccgggg tcgccgtgca
     3781 tacgcgggcc ctcgaccggg atgagcggga ccgggccctg gcgcacttcg aatcggtctt
     3841 ccggcgcgcg gcggaggggg cggggctgcg gctcgacgtg ctcgacggcg gcgccgaggc
     3901 gcacgcggcc gggcgcgaca aggggacggc gctgcgcgcg ctcctcgccg cgctcccctc
     3961 cgggacggtg ccggtctacg tcggcgacga cgtcaccgac gagcccgcgt tcgaggcggc
     4021 gcaggagcgc ggcttcggga tcaaggttgg ccccgccgcc acccgggcgc gccacgcgct
     4081 tgacggaccg cacgccgtgg cgggcctgct cgccggcctc ctgcgcgcgc tcgaggagac
     4141 ggcgtgagcc ggctcatcgt cgtctcgaac cggctcccgg caccgcccgc caccgggccg
     4201 cacctcgtcg ggcgggaaac cacggtcggc ggtctggccg cggcgatcct cggggcgctg
     4261 caggggcggg ccggatccct ctgggtcgga tggaacggac ggacggcggc gtcgggacgc
     4321 gccgccggca ccctcctgca ccaccggcgc gaaggggtcg atctcgcctc gttcccgctg
     4381 acctcggccg agctggacgg ctactaccac ggcttctgca atcagacgct gtggccggtc
     4441 ctgcactgct tccaggggcg ggtgcgcacg cgtctgaggg acgaggcggc ctacgacgcc
     4501 gtgcaggggc gcttcgccga tctgctcatg ccgatgctgc ggcgcgacga cctcgtgtgg
     4561 gtgcacgact accacctgat cccgctcggg cgggagctgc gccgccgcgg ctggcgcggc
     4621 cgcatcggct tcttcctgca cacgccgttt ccgccgctcg agctgtggga ggtgctgccg
     4681 cgtccgcgcg gaatgctcga ggcgctcttc gaatacgacg tcgtcggctt ccacgtgcag
     4741 ggcttcctcg acaactacgt ctactgcacc cgccgcctcc tcggcgccga ctgggacggg
     4801 cgtcggctgg cgcaggccgg gcgtgcgcag atcgcctcgg tctacccggt cggcatcgac
     4861 ccggatgcct ggcggccgca cgccggccat gggcccgccg cgggcgacgg cggggaggac
     4921 gcggccgcca gggtcggcga ggcgagcgtc gccaacctca aggtgctgcg cgggcttcag
     4981 gacaggcgga tgatcctcgg cgtcgatcgg ctcgactaca ccaaggggat cccgcagcgg
     5041 ctgcaggcgg tcgagcgctt cttccgccgc cgccccgagt ggcggcgccg cgcggtctac
     5101 gtccaggtcg cctcccccag ccgcgtccag atcccggagt acgccgagca gcggcgccgc
     5161 atcgaggcga ccctcggccg ggtcaacggc gagctctccg agccggactg gacgccgatc
     5221 cgctacctct accgggcgta cgatcggccg ctcctggcgc ggctctaccg tgaggcggac
     5281 gtcgggctgg tgaccccctt gcgcgacggc atgaacctcg tggcgaagga gtacgtcgcg
     5341 gcgcaggacc cggccgatcc cggcgtcctc attctcagcc acaccgccgg cgccgcccag
     5401 cagctcaagg acgcggtgct ggtgaacgcc ttcgtcccgg ccgacgtggc ggaagggatc
     5461 gccttcgccc tggagatgcc gctcgccgag aggcagcgcc ggcacgggcg cctgctcgcg
     5521 accgtggtcg attcgacggc ggaggtctgg gccgatcgct tcgtcaacga cctggagcgc
     5581 gccgtcgccg cgtcccccta ccaggtcgcg ggaccggagt tcgtcccccc gagcacgcag
     5641 atataatccc cctctttttg gtgggggaat cgatgccgcg catgtggatc ggcgggcgct
     5701 tcaccgacag cgtctcgcac cgcaccttcc cggtggacaa cccggccaac gagcggatcc
     5761 tggcccgcgt gccgcgcgcc gacgaccggg acgcggaccg cgccgtggac gccgcagccg
     5821 aggcgttcag gcgctggcgg caggtctccg ggctcgaacg ggcgaacctc atgcaccagt
     5881 tcgcggcgcg cctgcgggcg aagcacgtgg cgatcgcacg ccagctcacg ctcgaagggg
     5941 ggaagcccta catcgagaac ctcgacgaga tcgagtggtg cgccggcgtc ttcgactact
     6001 acggcgagat cgcccgcgac cagatgggga aggtgatcgc cccggtcttc ccgcaccagg
     6061 tgaacttcgt ccgcaaggag ccgtacggcg tcgtggtgtg catcgtgccg tggaactacc
     6121 cgctcctgct cctctcctgg aaggcggcgg cggcgatcgc ggcgggcaac acgctggtcg
     6181 tcaagccgtc ggaggagacg ccgctttcga ccctgatgct ggcggaggac ttcgacctct
     6241 tcggaaaagg ggtcgtcaac atcgtcaccg ggttcggtcc ggaggtgggg gagccgctcg
     6301 tcacccaccg ggacgtgtcc ctcatcgcct tcaccggcag catcgccacg gggaagaaga
     6361 tcgccgccgc ggccgcgccg cacctcaaga agctcaacct cgagctgggc ggcaacgatc
     6421 cgttcatcgt ctgcgacgac gtcgacgtgg cgatcgccgc caaggcggcc gcctgggccg
     6481 cgttcctcaa catggggcag gtctgcacct ccggggagcg cttctacgtc ttcgagcggg
     6541 tgtacgagga gttcgtcgag cgcttcatcg ccattgcgaa ggggctgagg ctcggcgacc
     6601 cgatggggcc ggacatcgac tgcggcccgc tcatccgcga gaaccaccgg ctgcagatcg
     6661 agcggaagat cgaggaggcg gtcgcccagg gggcgaccat ccgctgcggc ggccggcgcc
     6721 ctgcgaaact caagaagggg ttcttcttcg agccgaccgt tctcaccgac gtcacgccgt
     6781 cgatgaccct gatgaagagc gagacgttcg ggccgatcgc cccgatcgtt ccggtcaagg
     6841 ggatcgagga ggccgtgacg ctggccgacg attcggaata cggactcggc gcgaacatct
     6901 acaccaacaa cctcgagtgg gcgatgttcg cgatggagcg gatccacgcc gggacgttct
     6961 ggatcaacga tccgctcacc gacaacgacg ccgggccgtt cggcggcatg cggatgtcgg
     7021 ggatgggccg ggagctcggg atcgaggggc tcgacgcctt ccgcgagccg aagcacgttc
     7081 acatggatta ccgcatcgag gcgaagccgt actggtatcc ttaccgctgg aaaggggtgc
     7141 cgggggcgcc cgcgccgcgc cggaagagca aggcgcgccc gcgcgcgcgc cgctgaggat
     7201 ggcgatgaag aagcacatcg tcctgaagac gccgatcccg ggaccgcgct cgcagcaggc
     7261 cttcgaagcg cgccgcgccg cggtgccgcg cggcgcctac aacatcacgc cggtcgccat
     7321 cgcgaaaggg gagggggcga tcctcgagga cctggacggc aaccgcttca tcgacttcgc
     7381 cggcggtatc ggcgtgctca acgcgggcca cgcgccgccc gcggtcgtga aagcggtgca
     7441 gcaacaggcg gagaaattcc tgcactcctg cttccacatc accatcaacg agccgtatct
     7501 gcgcctcgcc gaggcgatga accggatcac ccccggcgcg ttccccaaga agacgctcct
     7561 ggtcaacagc ggcgccgagg cggtcgagaa cgccgtcaag atcgcccggg gtgcgacccg
     7621 gcggcccggg atcatctgct tcgaggacgc cttccacggg cgcaccaaca tggcgctgtc
     7681 gctgacgagc aagacgcacc cctacaagga gcacttcggg ccgttcaact ccgaggtgca
     7741 ccggatgccg tacgcctact gctaccgctg cccgtggggg aagaagtacc ccggctgcaa
     7801 catggagtgc gcccatttcc tgaccgactt cttcaaacgc tacgtcgagc cggacacggt
     7861 ggcggcgatc ctggtcgagc cgatcctcgg ggagggaggg ttcgtcaccc cgccgccgga
     7921 gtactacccg atcctcacgc gcctggcgcg cgagcggggg atcctgatca tcgccgacga
     7981 gatccagacc ggcttcggcc gcaccggccg catgtgggcc tgcgagcact acgggctcga
     8041 gccggacatc ctggtcgctt cgaagtcgat cgcctcgggt ctgccgatcg ccgcggtcac
     8101 ggggcgggcg gagatcatgg acgcgccgat ggaggggagt ctcggcggca cctttggcgg
     8161 caacccggtg gcggcggccg ctgccctcgc caccctcgag cagttcgaga agcaggatct
     8221 gcccgggcgg gcgcgccgca tcggcgaccg gatccgcgcc cgcttcgagg agctctggaa
     8281 gaagtgcccg gccgtgggga acgtccgggg cctgggcgcc atgatggcga tcgagttcgt
     8341 caaggatcgt gccaccaagg agccggcgaa ggagatggtg cagacgctcg tgcgcctgtg
     8401 ctacgagcgc ggcctcatca ccattccggc cgggacctac ggcaacgtct tccgggcgct
     8461 gatgccgctc gtcatcaccg acgagcagct cgacgagggg ctcgacgtcc tcgaggcggc
     8521 gatcctcgaa gcggcccagc cgatcatggc gacggcgacg ccggccgcag cgattcccgc
     8581 cggagccccg acgccgcgca cctgagtcgg cttacagcca gcggcgcacc cgcgtcttga
     8641 aggcgaggta ctcggtcccg aagagccgct ccatcatcgc ctcttcgtgg acgatgaagc
     8701 gggtctggat gaaatgcgcg aacagcggcg ggacgacgaa cggcgaaagg gtgccgaggc
     8761 ggatcgcgac gccggagagg accacgacca tcccgagata catcggattg cgggtgtaac
     8821 ggtagggccc ttcgagcacc aggcgggtcg aacgctcgaa cggcttgacc ggcgtcccga
     8881 gccgatcgaa cagcgccgcc gcccgcaccg tgagcgctcc gccgacaacg aacggcagga
     8941 gccccagcca ggtccacggc ggcgggatca gccgccgcac cggcaggatg aagtgaagca
     9001 ggagcatcac cacgagggcc aggaggaagt agacaggagg atacagccgc cgcgtcggga
     9061 acgtctggac gggatcgctc atgctctcgc cccccccgat cgctcttccc tacccacgga
     9121 agacgaaata gaccgcgccg cacaggcata atgcggccca caggtcattc aggcgcagcg
     9181 gccaggatct tgagctgccc gacgctcatc gtctggtgcc cgatccggtt ggccgggacc
     9241 tgcgccaggt actcgaagag cgcgatcccc cacgacacga tcgccgcgac gtaccacgcg
     9301 ct
//
LOCUS       DASRIQ010000438         4764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1229553, whole genome shotgun sequence.
ACCESSION   DASRIQ010000438 DASRIQ010000000
VERSION     DASRIQ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229553"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFD06_10705"
     CDS             complement(<1..835)
                     /locus_tag="VFD06_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04039.1"
                     /translation="MTVQRLFAIALIIVGTGVGWFILGGSVAIRTGASDQHLAREVEQ
                     IWGGTHRQVAPAVWYERSRIVTEPAKAPDGTPLAGGGTVSRAVVDRVPLAIASSRITA
                     ALNLEHRRKGLLWYDTYDVAFSARYLLRNTSPEHETVFARFEFPSKDGLYDAFALKVG
                     GAGLDMSDPEQGMTAQVPMAPGAEVPLEVSYKSRGLGAWTYAFAPAGVAQVHDFDIQV
                     TTNCDGVDFPAGSLSPTSKARDKDGWRLTWKFENLVTGQHIGVDLPNRLNPGPVAARI
                     IF"
     gene            complement(980..2386)
                     /locus_tag="VFD06_10710"
     CDS             complement(980..2386)
                     /locus_tag="VFD06_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZN04040.1"
                     /translation="MKPRFRFEEFTVSPEERVVTRAGVEVPLIPRYFDLLVLLLEERH
                     RAVTRDEILESVWSDVVVSDGALSQAIRTLRRALGDAAREPIYIRTVSRHGYRFVHPG
                     VTEEAVEGDGRPPAGPRFPATPPPAGPAAGSRTPVPEAAPAAGGDPFEPVVDRLLEGN
                     GEGRLEAAETLHQLGTDEALRRIERRAGAAQARALMREARWAIPGAGPVPLLGRSGWP
                     KAVLFLVGFRLRRAARIAGRRLGGAAVGGAMAGGIAGILGACALRLGAVPGGTPELAL
                     MLAGVGTVLGGVGAAGVGAGLAFAEAIARSLRGPALVLCGALGGGVTGWVLHALGRSL
                     LTQMFGQDVPRLGGGLEGLALGAAAGLAYALGTPRPHGGGMATPRGGRRVAVALATGA
                     CCGAAGVLAIHAGGNLVGTSLNLLAEAYSGSQVGLTPMARLLGEAEAGPLTRGLSAGL
                     EGLFFGSGLVFGLTRRPR"
     gene            complement(2470..>4764)
                     /locus_tag="VFD06_10715"
     CDS             complement(2470..>4764)
                     /locus_tag="VFD06_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZN04041.1"
                     /translation="APMLLGGSVARASTCEQPDLLSPVTTLGSADSSWRGPSMAKGSD
                     GYALVTWRWPQTQILFQRLSSTGVVIGAAQLGLPGAQGSLPHDPIVVSAGTGYGLAWY
                     DDGRDSVVFVRVTSTGVPIPPFLEFPAYGYVLGMPGLAWTGDEFALAWLDPQNRLVFR
                     RIDASGSLVGDVVMVSERPALDVRLVAGEAGFAVVWMGTRDTSPPYYLSYPVFRALGQ
                     DGSPAGQETFLTPLFVSGGFADLAWIGDRYAVAWTAWDSGLWSSFASPDGILDPPIQV
                     TPDVSLYSSAPVPALGWTGTELTIAWGRSAGDRRIQTRRISPQGVFLGDPVSLTHAER
                     ESTVGGLIWTGDRFAMSWVIFGGEARFGFVACDCADADHDDHSVCRGDCDDAASGVHP
                     GASEICNAMDDDCDGGADEGLDVSFTCGIGSCQRTVVFCVNGAVSSCVPGPPSAEVCN
                     LIDDDCDGTADNGDADGDGSFDCVDCGPTVGSIHPGAVETCNGIDDDCNEIADDLSGV
                     ADLDGDGVAGACDDCPSISNPGQEDLDHDGLGDICDNCPAASNPSQADTDGDGTADAC
                     DLCPLSPYPTTDADGDGLGAACDNCASVANPGQENSDFDAFGDVCDRCSGFVNISNDD
                     DDGDTLGNLCDNCRFNPNADQTDADEDGEGDACDLDDGWLMIWVTRPDEVNWDAEPTF
                     FLYDVYRGDIDVLKATGESTQDPDVVPLAGRFCGLLDPFLVDDPPPLGKAVFYLVAVE
                     TAFGYEGIGNDSAGNPRNNSHPCP"
BASE COUNT          786 a         1730 c         1599 g          649 t
ORIGIN      
        1 agaagatgat gcgggccgcc accgggcccg ggttgagccg gttcggcagg tcgacgccga
       61 tgtgctggcc ggtcacgagg ttctcgaact tccaggtcag ccgccatcca tccttgtccc
      121 tggccttgct cgtcggggac aggctgccgg ccgggaaatc gacgccgtcg cagttggtcg
      181 tgacctggat gtcgaagtcg tggacctgcg ccacgccagc cggcgcgaaa gcgtaggtcc
      241 acgcgccgag gccgcgtgac ttgtacgaaa cctcgagcgg cacctccgcc cccggggcca
      301 tcgggacctg cgcggtcatc ccctgttccg gatcggacat gtcgaggccc gcgccgccga
      361 ccttcagggc aaaggcgtcg tagagtccgt ccttgctcgg gaactcgaac cgggcgaaga
      421 ccgtctcatg ctccggcgac gtgttgcgca gcaggtaacg ggccgagaag gcgacgtcgt
      481 aggtgtcgta ccagaggagg cccttgcgcc ggtgctcgag attcagggcc gccgtgatgc
      541 ggctggacgc gatcgcaagc ggaacgcggt cgacgaccgc cctcgagacc gtgccgcccc
      601 ctgcgagcgg ggtcccgtcg ggcgccttgg ccggctcggt cacgatgcgg ctccgctcgt
      661 accagacggc cggggccacc tgccggtgcg tcccgcccca gatctgctcg acctcgcggg
      721 ccagatgctg atccgaggcc cctgtccgga tcgcgaccga ccctccgagg atgaaccagc
      781 cgacccccgt gccgacgatg atcagggcga tggcgaacag gcgctggacg gtcatgaagc
      841 ctcctgtctc atgccggccg atcccggaag cggggggcgg gctcgccggc gaatgtgccg
      901 cgggggaaga gaaaagacct gagggcccgg tgagcagtcg gtgagaaggg gctgaggacc
      961 caggagcctg tccgggctcc taacggggtc ggcgggtcag gccgaacacc agccccgacc
     1021 cgaagaaaag accctccagg ccggccgaaa gcccgcgtgt cagcggtcca gcctcggcct
     1081 caccgagcag ccgggccatc ggggtcagcc cgacctgcga gccgctgtag gcttcggcga
     1141 ggagattcag gctcgtcccg acgagattcc cgcccgcatg gatggcgagc acccccgccg
     1201 ctccgcagca ggccccagtg gccagggcca cggcgacccg cctcccgccg cgcggcgtgg
     1261 ccatcccgcc gccgtgagga cgcggggtac cgagggcata cgcgagcccg gcagcggccc
     1321 ccagcgccag cccttcgagc ccccctccga gccgaggcac atcctggccg aacatctggg
     1381 tcaggagtga gcgccccagc gcgtgaagga cccacccggt cacaccaccc ccgagtgcgc
     1441 cgcaaagcac cagcgccgga ccgcgcagag acctggcgat ggcctcggcg aaggcgaggc
     1501 ccgccccgac ccccgccgcc ccgaccccgc cgaggaccgt gccgaccccc gccagcatca
     1561 gagccagctc cggtgtgccg ccggggaccg ccccgagcct cagggcgcac gcacccagga
     1621 tccccgcgat tccgccggcc atcgccccgc cgactgccgc cccgccgagg cgccggccgg
     1681 cgatccgcgc cgcccggcgc agccggaagc cgacgaggaa caggaccgcc ttcggccatc
     1741 ccgaccggcc gagcagcggc accggccccg cccccggaat cgcccaccgc gcctcgcgca
     1801 tcagggcgcg ggcctgcgcg gccccggccc gccgttcgat gcggcggagc gcttcgtcgg
     1861 tcccgagctg gtgcagcgtc tccgccgcct ccagccgccc ctcgccgttc ccctcgagca
     1921 gccgatcgac gaccggctcg aacggatccc ctcccgccgc gggagcggct tcagggacag
     1981 gtgtccggga gccggccgcg gggcccgcgg gcggcggcgt tgccgggaac cggggcccgg
     2041 ccggtggccg gccgtcccct tcgaccgctt cctcggtcac accgggatgg acgaagcggt
     2101 agccgtggcg cgagacggtg cggatgtaga tcggctcacg cgccgcgtcg ccgagggcgc
     2161 ggcgcagcgt gcggatcgcc tggctgagcg cgccgtcgga gacgacgacg tcgctccaga
     2221 cgctctcgag gatctcgtcg cgggtcaccg cccggtgccg ctcttcgagc agcagcacca
     2281 gcaggtcgaa gtagcgcgga atgagcggca cctcgacgcc ggcgcgcgtc acgacccgct
     2341 cctccggcga gaccgtgaac tcctcgaacc ggaatcgggg cttcatgcgg aagcaggatt
     2401 ataggggcac gccctgcatc ctccgcgaac ggggccctcc tcgacgacct gccggagctg
     2461 acccttcccc tatgggcagg ggtgggagtt gttccgggga ttccctgcgc tgtcgttgcc
     2521 aatcccttcg tacccgaaag ccgtctcgac tgccacgagg tagaagaccg ccttgccgag
     2581 cggcggcggg tcatcgacca ggaagggatc gaggaggccg cagaaccggc cggccagcgg
     2641 cacgacgtcc ggatcctgcg tcgactctcc cgtggccttc aggacgtcga tgtcgccccg
     2701 gtacacgtcg tagaggaaga aggtcggctc cgcgtcccag ttcacctcgt cgggtctcgt
     2761 cacccagatc atcagccagc cgtcgtcgag atcgcaggcg tccccctctc catcctcgtc
     2821 ggcgtcggtc tggtcagcat tcgggttgaa gcggcagttg tcgcacaggt tgccgagcgt
     2881 gtcgccgtcg tcgtcgtcgt tcgagatgtt cacgaatccc gaacagcggt cgcagacgtc
     2941 cccgaaggcg tcgaagtcgg agttctcctg cccgggattc gccaccgacg cgcagttgtc
     3001 acaggctgcg ccgaggccgt ccccgtccgc gtcggtcgtc ggatacgggc tcagaggaca
     3061 gagatcgcag gcatcggcgg ttccgtcgcc atcggtgtcc gcctgcgacg ggttgctcgc
     3121 ggccggacag ttgtcgcaga tgtcgccgag gccgtcgtga tccaggtcct cctgcccggg
     3181 attcgagatg gagggacaat cgtcgcaggc gccggcgacg ccgtcgccgt cgaggtccgc
     3241 aacaccggag aggtcgtcgg cgatctcgtt gcagtcatca tcgatcccgt tgcaggtttc
     3301 caccgcaccc ggatggatcg agccgaccgt cggaccgcag tcgacgcaat cgaacgatcc
     3361 gtcgccgtcc gcatcgccgt tgtccgccgt cccgtcgcaa tcgtcatcga tgaggttgca
     3421 gacctcggcg gaaggggggc cgggcacgca ggaactgacg gctccgttca cgcagaagac
     3481 caccgtgcgc tggcaagacc cgatcccgca ggtgaaggac acgtccagcc cttcgtcagc
     3541 cccgccatca cagtcgtcat ccatcgcgtt gcagatctcg gacgcgcccg gatggacccc
     3601 gctcgcggcg tcgtcgcagt cgccccggca gacgctgtga tcatcgtggt cggcgtccgc
     3661 gcagtcgcag gccacgaacc cgaaccgggc ttcgccgccg aagatcaccc acgacatcgc
     3721 aaagcgatcc cctgtccaga tcagtccgcc gaccgtcgat tcgcgttcgg catgcgtcaa
     3781 cgacaccgga tcgccgagga acactccctg tggactgatg cgcctggtct ggatgcggcg
     3841 gtcgcctgcc gatcgtcccc aggcaatcgt cagttcggtt ccggtccagc cgagcgccgg
     3901 cactggcgca ctgctgtaca gggacacgtc gggggtcact tggattggcg ggtccagaat
     3961 gccatccggc gacgcaaacg acgaccagag gccggagtcc caagcggtcc aggcgaccgc
     4021 gtaccggtcg ccgatccacg ccaggtccgc gaaacccccg ctgacaaaca gcggagtcag
     4081 gaaggtctcc tgacccgccg gactcccatc ttggccaagg gcacggaaaa ccgggtaact
     4141 gaggtagtag ggtggcgacg tatccctggt gcccatccat acgacggcga accccgcctc
     4201 tcccgcaacg aggcgcacat caagagccgg gcgctccgag accatcacca catcgccgac
     4261 cagggacccg ctcgcgtcga tgcggcggaa caccagacga ttctgcggat cgagccaggc
     4321 gagcgcgaac tcgtcgcccg tccatgcgag ccccggcatt cccaggacat acccgtacgc
     4381 cgggaactcc aggaacggcg ggatgggaac gccagtcgag gtcacgcgaa cgaacacgac
     4441 cgaatcgcga ccatcgtcgt accacgccag gccataacct gtcccggcgc tgaccacgat
     4501 cggatcgtgc ggcaatgagc cctgagcgcc gggcaagcca agctgcgcgg cgcctatcac
     4561 gactcccgtg ctcgaaaggc gctggaacag gatctgcgtc tggggccacc tccaggtgac
     4621 gagagcgtag ccgtcgctcc ccttcgccat ggaaggcccg cgccaggagg aatcggcaga
     4681 ccccagggtc gtcactgggg aaaggagatc gggctgctcg cacgtcgaag cgcgcgccac
     4741 ggatccacca aggagcatag gggc
//
LOCUS       DASRIQ010000439         8279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3847550, whole genome shotgun sequence.
ACCESSION   DASRIQ010000439 DASRIQ010000000
VERSION     DASRIQ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3847550"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2669)
                     /locus_tag="VFD06_10720"
     CDS             complement(<1..2669)
                     /locus_tag="VFD06_10720"
                     /inference="COORDINATES: protein motif:HMM:NF017369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HZN04042.1"
                     /translation="MSRRTVLRTGIVVAALCGLTSLGEASRPRTIGPGDPPPGSDDRT
                     RAERVSALTAEVLNLLAEIEAAAPGAAAALKERGRILLELRRTEFEPLIDEDPGGALA
                     SALDTESVERLGAVIPDARPDLETVGRFQGVAEVLIADDFERGKAETIVTIALGERRV
                     RVQFSGAKPEGLASGTMLAVDGVLMGDRVAASSGEILAGAGAAALAAPACSTLGDQKV
                     AVLLVNMPGATLPPYLTPENVRNFFFGTDGGKSLDGYWREASYGLTSASGGVFGPYLL
                     GQDYNCDQYPGIRVAAIQAADADVDFRDYNRIFIIFPRLTSCWFTGLGELSCGLLSSP
                     GDGSFIASTSWFDGTTWANHNAPRDLGVFVAAHEGGHNFGLQHSFWRSFNGQPLGVPG
                     SQPADSEYTDPFSAMGRPNAGHYAAPHKLRLGWLDPATEVLTVTTSGTYPILPYEPPS
                     SGVKALKVQRGAESEWLWLESRTTQSAYDCQPGTLLNCNGALVHYDVPNSAATYLLDF
                     TPGSGFFVNDAPIPAGTTWFDVYTGLSLTPTYGMGVLNVAADYGPRTCHRPPTVQVLP
                     AGQSVSNGGGVSFTVTVTNVDEPTCPNATYTLSSAPAGWSGRFTPASLSIPPGGSANS
                     ALVMTVPRSTEAGPQTVTASAAWTTVTGTGTQTVNVVSDCVLGNPIVRVLPDTQSVAI
                     NETAFFTVEIDSTDTAGCGVHRFDITSALPTGWSGVITPSFINLTGNGPVGTLQMRKF
                     IPAETPIGSTHTVDATATSRPGTPTAPHVGNDTAALTIAEPCTHADPTLTAIPTAQIA
                     PPGDSPYFQGFVHNNDSPTCAPVTFTIVVTGPSGWPVFPESDCIPPCPPTVLVPPGQS
                     RDVWFGATVPVGTAPGSYGVTLTANAPGGQSGST"
     gene            2882..3730
                     /locus_tag="VFD06_10725"
     CDS             2882..3730
                     /locus_tag="VFD06_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN04043.1"
                     /translation="MPDSDDRALLDAIAPGGPAAWQAFLDSCSATVFRVVRLFADTYD
                     ERMDLFLFVCSRLHADDMRRLRSFRYRPEAPCRFSTWLTVVVKNLAVDYLRSKQGRDR
                     PFRNVEAMDEIDRLVFDYHLRDGRPLREVRQILGTRHGLGVGDADLAGRAARVEAALS
                     ASQRWRLLARLAERREHLPLDPVSGAAVAVTHEAITPTDAGENPEQALRHREEGRALR
                     LALLAIPPRERLAVTLRHRDGLSAREVAAVLKVTPEEAERLARAGAQNLRERLGGATT
                     VAVRTR"
     gene            3727..4401
                     /locus_tag="VFD06_10730"
     CDS             3727..4401
                     /locus_tag="VFD06_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN04044.1"
                     /translation="MIAAGHPDEQTTAAYLEGRLPQAARDGVEAHLAACDTCRSGVAL
                     LRLGEEESDETVPPAALRRARGLAAGQTRRFVLPAGLAAGLLAALGLTIWLGGRGANR
                     AAGGPEGAAEAPVERDAGAPALEAISPARGDVVPAGSLAFRWKTVAGADRYVVTLLDA
                     GGAGIAAIETGPPTGPLAKNESTIALDWPADRPLPAAGTYLWTVRALALDRVLAETRP
                     VPFEVR"
     gene            4398..7646
                     /locus_tag="VFD06_10735"
     CDS             4398..7646
                     /locus_tag="VFD06_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZN04045.1"
                     /translation="MSRGRAAAAALLLGLLAWGDDGGVRAADEKMSAPFLVKARAAHA
                     RGDHRAVERLVAADRWVAYDAFYETLKQDDETAAGGLAEVLATAYTRAFEDSVLEDAL
                     RLARSWTPDQRAHRREAFALRVAARVSAQEGRLDAAAEAYGRILQTYVDLGDVREQAW
                     SLSNLGGIAVMQGQSREGLEYLDRAVELALQARDRSPIGGAAANRAYGLDDLGETEAA
                     LAAYDHALTISRQTGDLQTEMRALASRGSLLTREGRPDDATADFLAAAAVAERAQDFE
                     VQSVAWLNLATLDQYRGDTETQVRHLKRSVEAGRRGGLPLLESDANLILGRIARYQGN
                     HVEARRRLEASRAALAGSDDVLRLTDLDLEEASLRMDQGRDREALELLTASERRLEGL
                     ELGGSRAAILVARAVALYHLGDYDGAIGTLRASVDEAKAKDHPALEGMARAHLGYLLF
                     TLGDAAGGIAQLEEAMRLDERLGDRPGRGLHLEAIGFIRYRTGDLAGARQALEEALTL
                     LPPEVDPDGRAETMKSLGLVLLATGGSRSSVDGAETRRRGADLLRQARETFTRLRNDK
                     AAFQANLLEADALLGARDAVSARAALLRAERIPSGRAAREDLWLADHLRGRLAELERD
                     PAMARRRYQRAVAAVENLRGTVRPAPWRAALLEDRIAPYRALVRLLREEGDEAAAWKV
                     ARAAKARTFVESLLLPDMPPTLSVAFSRETGPPEGFAPSGFEAASDGRLAALLAPDER
                     LIDFFADGREVIAFVLGGSRLVARTLRVDAAADMALARWPGRPGQADAAVTAAWRRVM
                     GRIGTALFEPLAADLDGAKRLLIVPGGPLHGVPFAAVGYRGRRLVDQFEISVLPAAEA
                     LLSRHRHPAGTGALVIGDPASGEPRLPAAAREAQAVGALAGGSRVLTGAAATEAAFRR
                     LAPASDWIHVAAHGRIDPIAPARTQLALAAGADADGRLEAGEVAAMDLTASLVVLTGC
                     GTGVEAGLARGDAPADERAGLPRAFLQAGAGTVVAALWEMDDAASAAFIPRLYQGLRS
                     REPASALAGLQRDLASGRIRGDDGAPLDHPYYWAGLLSWGAGLAPTSRTAAR"
     gene            7670..>8279
                     /locus_tag="VFD06_10740"
     CDS             7670..>8279
                     /locus_tag="VFD06_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZN04046.1"
                     /translation="MRDATRVIHAGRREPVQGAPFLPGPVFAAPYHLKGDPAGVPYTY
                     GRFDNPTWSGFEQALGELEEGEAVVYASGMAASAAVLGSALQPGDTVVLPEDGYYTTR
                     LLAVGYFARAGITVRKAPTANDAQGGFLEGAKLLWLESPTNPGLDVCDIRALAESARM
                     RGAVVVVDNTTATVLGQRPLRLGADLSLASDTKGLTGHSDVVL"
BASE COUNT         1092 a         2960 c         3073 g         1154 t
ORIGIN      
        1 gtcgagccgc tctgccctcc tggagcgttg gccgtcaggg tgacgccgta actccccggc
       61 gccgttccga ccggcaccgt cgcaccgaac cagacatccc ggctctgacc cggcggaacc
      121 aggaccgtcg gagggcaggg aggaatgcag tcgctctcgg ggaagaccgg ccaccccgag
      181 ggacccgtga ccacgatcgt gaacgtgacc ggggcgcagg tcggcgagtc gttgttgtgc
      241 acgaacccct ggaagtaggg gctgtctccc gggggagcga tttgcgccgt ggggatggcg
      301 gtgagcgtcg ggtcggcgtg tgtgcagggc tcggcgatcg tcagcgccgc cgtgtcgttc
      361 ccgacgtggg gggcggtggg cgttcccggc cgcgatgtgg ccgtggcgtc gacggtgtgc
      421 gtggacccga tcggcgtttc ggcggggatg aacttgcgca tctgcagcgt cccgacaggg
      481 ccgttccccg tcaggttgat gaacgacggc gtgatcaccc ccgaccagcc ggtcggcagg
      541 gccgaagtga tgtcgaagcg gtgcacgccg cagcccgccg tgtcggtgct gtcgatctcg
      601 accgtgaaga aggcggtctc gttgatcgcc acgctctgcg tgtcggggag gacgcggacg
      661 atcggattgc cgaggacgca gtcggagacg acgttcacag tctgggtccc ggtgccggtc
      721 accgtcgtcc aggcggcgga cgcggtcacg gtctgtggcc cagcctccgt ggagcgcggg
      781 accgtcatga cgagagcgct gttggccgag ccgcccgggg gaatcgacag ggacgccggt
      841 gtgaaccggc cggaccatcc ggccggggcc gaggagagcg tgtaggtcgc gttgggacag
      901 gtcggctcgt ccacgttggt cacggtcacg gtgaaggaga ctcccccgcc gttgctgacc
      961 gactggcccg ccggcaggac ctgcacggtg ggcggtcggt ggcaggtgcg cggaccgtag
     1021 tccgccgcca cgttgagcac ccccatgcca taggtcggcg tgagcgagag cccggtgtag
     1081 acgtcgaacc aggtcgtccc cgccgggatg ggcgcgtcgt tgacgaagaa tccggagccc
     1141 ggcgtgaagt cgagcaggta cgtggcggcg ctgttcggca catcgtagtg gacgagggcg
     1201 ccgttgcagt tcaggagcgt cccaggctga cagtcgtagg cgctttgcgt ggtgcgggat
     1261 tcgagccaga gccactccga ctcggcgccg cgctgcacct tgagggcctt gacgccgctc
     1321 gagggcggct cgtacgggag gattggatag gtgccgctcg tcgtcaccgt gagcacttcg
     1381 gtcgccgggt cgagccagcc gagccgcagc ttgtgcggcg cggcgtagtg cccggcgttg
     1441 ggccgcccca tggcgctgaa cggatcggtg tactccgagt cggccggctg cgagccgggc
     1501 acccccagtg gctggccgtt gaaggagcgc cagaaggagt gctgcagacc gaagttgtgc
     1561 cccccttcgt gcgccgcgac gaagacgccg aggtcgcgcg gggcgttatg gttggcccag
     1621 gtggtgccgt cgaaccacga cgtcgaggcg atgaacgacc cgtcgccggg tgacgagagc
     1681 aacccacaag atagctcccc caggcccgtg aaccagcagg aggtgagccg cggaaagatg
     1741 atgaagatgc ggttgtagtc gcggaaatcg acgtcggcgt cagcggcctg gatcgccgcg
     1801 acccttatcc ccggatactg atcgcagttg tagtcctggc ccagcaggta cgggccgaag
     1861 accccgcccg aggcggaggt gagcccgtag gaggcctcgc gccagtagcc gtcgagcgac
     1921 ttgccgccgt cggttccgaa aaagaagttg cggacgtttt cgggggtcag gtacggcgga
     1981 agagtggcgc cgggcatgtt gaccagcagc acggcgacct tctgatcgcc gagggtggag
     2041 caggcgggcg cggccagagc ggccgctccc gcccctgcca ggatctcgcc cgaggaggcg
     2101 gcgacgcgat cccccatgag gacgccgtcc accgccagca tcgtcccgct cgccagccct
     2161 tcgggcttcg cgccggagaa ctggacgcgg acgcgccgct cgccgagcgc gatggtgacg
     2221 atcgtctccg ccttgccgcg ctcgaagtcg tcggcgatca gcacctccgc gaccccctgg
     2281 aagcgcccga ccgtctccag atcggggcgc gcgtcgggga tgaccgcccc caggcgctcg
     2341 accgattccg tgtcgagcgc cgaagcgagc gctcccccgg gatcctcgtc gatgagcggc
     2401 tcgaactcgg tgcggcgcag ctccaggagg atccggcccc gctccttgag cgcggcggcg
     2461 gcgccggggg cggctgcctc gatctccgcg aggagattca ggacctccgc ggtcaaggcg
     2521 ctcacccgct cggcgcgggt ccggtcgtcc gatccgggcg gcgggtcccc cggcccgatg
     2581 gtgcgcggcc gcgacgcctc tccgaggctc gtcaggccgc agagggcggc gaccacaatt
     2641 ccagtccgta gcacggttcg tcggctcatg actccctccc gtccaaagca gcgaaaccag
     2701 cgagtgatgg ctcttctggc attcaacgga caggaatgcg ccggacggtc gggccggcct
     2761 caggtcccgg atcgatccgc ccgtggttgc cgcttcaggg ccctgtttgg taggctcgcg
     2821 ccatccgcca aagagcccat gcggcgccga ttcctcacgg cgccttcatc cgggaggtgc
     2881 gatgccggac agcgatgatc gagcgctcct cgacgccatc gcgcccgggg ggccggccgc
     2941 ctggcaggcc ttcctggact cctgttcggc caccgtcttc cgcgtggtgc ggctgttcgc
     3001 cgacacctac gacgagcgga tggatctctt ccttttcgtc tgctcgcggc tgcacgcgga
     3061 cgacatgcgc cggctgcgct cgttccgtta ccgccccgag gccccgtgcc gcttttccac
     3121 ctggctgacg gtggtcgtga agaatctggc cgttgattac ctcagatcga aacagggacg
     3181 cgaccgccca tttcgcaacg tggaggccat ggacgagatc gatcggctgg ttttcgacta
     3241 tcacctgcgc gacggccgtc cgctccggga ggtgcggcag atcctcggga cgcggcacgg
     3301 cctcggggta ggcgacgccg acctggctgg ccgggcggcg cgcgtggaag cggccctctc
     3361 ggccagtcag cgctggcggc tgctggcgcg cctggccgag cggcgggagc acctccccct
     3421 cgatccggtc tcgggcgcgg cggtggcggt gacgcacgag gcgatcacgc cgaccgatgc
     3481 cggcgagaat ccggagcagg cgctccgcca ccgcgaagag ggtcgggcgc tgcgtctcgc
     3541 gctcctggcg atcccgccgc gcgagcggct ggcggtgacg ctgcgccacc gggacggcct
     3601 gagcgcacgc gaggtggccg cggtcctcaa agtgacgccg gaggaggccg agcggctggc
     3661 gcgcgccggc gcccaaaacc tgcgcgagcg gctgggaggg gcgaccacgg tcgccgtgag
     3721 gacgcgatga ttgccgccgg ccatccggac gaacaaacca ctgccgccta tctcgaaggc
     3781 cggctgccgc aggcggcgcg cgatggcgtc gaggcgcacc tcgcggcctg cgacacctgt
     3841 cgctccggcg tcgcgctgct gcgcctcggg gaggaggagt cggacgaaac ggtcccccct
     3901 gcggcgttgc ggcgggcgcg ggggctcgct gcgggtcaga cccgccgatt cgtgctgccc
     3961 gccgggctcg ccgcgggact gctcgccgcg ctcgggctga cgatctggct cggaggccgc
     4021 ggtgccaacc gcgcggcggg aggccctgag ggcgccgcgg aggcgccggt cgagcgcgac
     4081 gccggagcgc cggccctcga ggcgatctcc cccgcgcgcg gcgacgtggt gcccgccggc
     4141 agcctggcgt tccgctggaa gaccgttgcc ggagccgatc gctacgtcgt cacgctgctc
     4201 gacgccggcg gggccgggat cgccgcgatc gagaccggac ctccgaccgg gcccctcgca
     4261 aagaacgagt ccacgatcgc gctcgactgg cccgccgatc gcccgcttcc ggccgcgggc
     4321 acctacctct ggaccgtgcg cgcgctcgcc ctggatcgcg tcctcgccga gacgcgcccg
     4381 gtcccgttcg aggtccgatg agccggggcc gcgccgcggc ggccgcgctc ctcctcggcc
     4441 tgctggcgtg gggggatgac ggcggtgtgc gcgcggccga cgagaaaatg tcggcgccgt
     4501 tcctggtgaa ggcgcgcgca gcgcacgccc gcggcgacca ccgtgcggtc gagcggctcg
     4561 tcgccgccga tcgctgggtc gcgtacgacg cgttctacga gaccctcaag caggacgatg
     4621 agacggccgc cggcgggctg gcggaagtcc tggcgaccgc gtatacccgc gccttcgagg
     4681 actccgtgct cgaggacgcc ctgagactcg cgcggtcatg gacccctgat cagcgcgccc
     4741 accgcaggga ggccttcgcg ctgcgcgtgg cggcgcgggt gtcggcgcag gagggccggc
     4801 tcgacgccgc ggcggaagcg tacggccgca tcctgcagac ctacgtggac ctgggggacg
     4861 tacgcgagca ggcgtggagc ctctcgaacc tcggcgggat cgcggtgatg caggggcaga
     4921 gccgggaggg gctcgagtac ctcgaccgcg cggtcgagtt ggcgctccaa gcgcgcgatc
     4981 ggtccccgat cggaggggcg gcggccaatc gcgcctacgg tctcgacgac ctgggggaga
     5041 ccgaagccgc cctcgccgcc tacgatcacg cgctgacgat ttcgcgccag accggcgacc
     5101 tgcagaccga gatgcgcgcg ctcgcctcgc gcggctcgct gctgaccagg gaggggcgcc
     5161 ccgacgatgc gaccgcggat ttcctcgcgg ccgcggccgt ggcggagcgc gcgcaggact
     5221 tcgaggtgca gtcggtcgcg tggctcaacc tggcgacgct cgatcagtac cgcggcgaca
     5281 cggaaaccca ggtccgtcac ctgaagcgct ccgtcgaggc cggccgccgc gggggactgc
     5341 cgctgctgga atcggatgcg aacctgatcc tggggcgcat cgcgcgctat caggggaatc
     5401 atgtggaggc ccggcgccgg ctcgaggcct cccgcgcggc gctcgcgggg tcggatgacg
     5461 tcctgcggct caccgacctc gacctcgagg aggcgagcct gcggatggac cagggccgcg
     5521 accgcgaggc gctcgagctg ctcaccgcct ccgagcgccg gctggaaggg ctcgagctgg
     5581 gggggagccg tgccgcgatc ctcgtggcgc gcgcggtcgc gctctatcac ctcggtgact
     5641 acgacggcgc catcgggacg ctgcgcgcct ccgtggacga ggcgaaggcc aaggatcatc
     5701 cggcgctcga ggggatggcg cgcgcgcacc tcggctacct gctcttcacg ctgggggacg
     5761 cggccggagg gatcgcgcag ctggaggagg cgatgcggct cgacgaacgc ctcggcgacc
     5821 gccccggacg cggtctccac ctcgaggcga tcggattcat ccgctaccgc acgggggacc
     5881 tcgccggcgc ccgtcaggcg ctggaggagg cgctcacgct cctgccgccc gaagtcgatc
     5941 ccgacggccg ggccgagacg atgaagagcc tcggcctcgt gctcctcgcc accggcggga
     6001 gccggagttc agtcgatggg gcggagacgc gtcgccgtgg cgccgatctg ctgcgccagg
     6061 cgcgtgagac gttcacccgg cttcggaacg acaaggccgc cttccaggcc aacctgctgg
     6121 aggccgacgc cctcctcggc gctcgcgacg ccgtctccgc ccgcgccgcg ttgctgcgcg
     6181 ccgagcgcat cccgtccgga cgcgccgcgc gcgaggacct gtggctcgcc gaccatctgc
     6241 gcggccgcct cgccgagctg gagcgggacc ccgccatggc gcggcgccgg taccagcgcg
     6301 ccgtcgccgc agtcgagaat cttcgcggca ccgtgcgccc ggccccgtgg cgcgccgccc
     6361 tcctcgagga tcggatcgcg ccgtaccggg cgctggtccg tctgctgcgc gaggagggag
     6421 acgaggcggc agcatggaag gtcgcgcgcg ccgccaaggc ccgcaccttc gtcgagagcc
     6481 tgctgctccc cgacatgccg ccgacgctct ccgtcgcttt ctctcgcgag accgggccgc
     6541 cggaaggttt cgccccgtca ggcttcgaag cggcttccga tggccggctc gccgcccttc
     6601 tcgcgccgga cgagcgtctc atcgacttct tcgccgacgg ccgcgaggtg atcgccttcg
     6661 tcctcggcgg aagccggctg gtggcgcgga ccctccgcgt cgacgccgcg gcggacatgg
     6721 cgctggcgcg ctggcccgga cgccccgggc aggcggacgc cgccgtgacc gcggcctggc
     6781 ggcgcgtcat ggggcgcatc ggaacggccc tgttcgagcc gctcgccgcc gatctcgacg
     6841 gcgcgaagag gctcctgatc gtccccggcg gcccgctcca cggcgtgccg ttcgcggccg
     6901 tggggtatcg cggcaggcgg ctcgtcgatc agttcgagat cagcgtgctg cccgccgccg
     6961 aggcgctcct gtcgcgccac cggcaccccg ccggcacggg ggcgctcgtc atcggcgatc
     7021 ccgcctcggg ggagccgcgc ctgcccgccg ccgcccgcga ggcgcaggcg gtcggagcgc
     7081 tcgccggcgg cagccgggtg ctgaccggtg ccgccgcgac cgaggccgcg ttccgccgcc
     7141 tggcccccgc gtccgactgg atccacgtcg cggcccacgg ccgcatcgat ccgatcgcgc
     7201 ccgcgcggac ccagcttgcc ctcgcggccg gcgccgacgc cgatggccgg ctcgaggcgg
     7261 gcgaagtcgc cgccatggac ctcacggcgt cgctcgtcgt cctgaccggc tgcggcaccg
     7321 gcgtcgaagc cggcctggcg cgcggcgatg cccccgccga cgagcgcgcc ggcctccccc
     7381 gcgcgttcct gcaggccggc gccggcaccg tcgtcgccgc cctctgggag atggacgacg
     7441 ccgccagcgc ggccttcatc ccccgccttt accaggggct gcgctcgcgc gagccggcct
     7501 ccgccctcgc cgggctgcag cgcgacctcg cctccggccg catccgcggc gatgacgggg
     7561 ccccgctcga ccatccttac tattgggccg gcctgctctc ctggggcgcc ggcctcgcgc
     7621 ccacgtcccg gaccgcggcg cgatgaacgg tcggcggtat gctggagcca tgcgcgacgc
     7681 gacccgggtc attcacgccg gacgacgcga gccggtccag ggggcgccgt ttctccccgg
     7741 gccggtgttc gcggcgccgt accacctcaa aggggatccg gccggagttc cctacaccta
     7801 cgggcggttc gacaacccga cctggtcagg cttcgagcag gcgctcggag agctggagga
     7861 gggagaggcg gttgtctacg cctccggcat ggcggcgagc gccgccgtgc tcggttcggc
     7921 gctccagccc ggggacaccg tggtcctgcc ggaggatggc tactacacga cgcggctcct
     7981 tgcagtcggg tacttcgccc gcgccggcat cacggtgcgc aaggctccga cggcgaacga
     8041 cgcccagggg ggcttcctcg agggagcgaa gctcctgtgg ctcgagagcc ccacgaatcc
     8101 ggggctcgac gtgtgcgaca tccgggcgct cgccgagtcg gcgcgcatgc gcggggcggt
     8161 cgtcgtggtg gacaacacga ccgcgaccgt cctcggccag cgtccgctcc ggctcggggc
     8221 cgatctctcg ctcgcctcag acaccaaggg gctcacgggg cattccgacg tggtgctcg
//
LOCUS       DASRIQ010000440         9706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_950937, whole genome shotgun sequence.
ACCESSION   DASRIQ010000440 DASRIQ010000000
VERSION     DASRIQ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950937"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFD06_10745"
     CDS             <1..288
                     /locus_tag="VFD06_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04047.1"
                     /translation="LDRSTPAATIENGTLPGARVRHGRLEELESLGAGRGDGPLLVVV
                     GRTAALAGDLAGPLAAGARLAAETGAETATDTVAPQDAAETAAIPFRRTGR"
     gene            285..2639
                     /locus_tag="VFD06_10750"
     CDS             285..2639
                     /locus_tag="VFD06_10750"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZN04048.1"
                     /translation="MNELDSTSLPPETAAEIANIESEIGRFLAGTLAPERFRSFRLAH
                     GIYGQRQPGVQMVRVKIPTGALHGAQLRLLADLVDDFSNGSAHLTTRQDVQFYYVRLE
                     RVPDMLRRLAAGGLTTREACGNSVRNVTACPLSGSLQDEAFDIRPYALATWRYLVRNA
                     FCQQMSRKFKIAFSACPEDCAATAIHDIGALGRVVFRDGVQKQGFRVVVGGGLGPSPF
                     VAQVLRDFVAPEDLLPTFKAILLAFAEQGNRRLKSKARLKFVLHRLGIERFRALVDEK
                     LAALSTAERDEADLARWLQDGDAAEAAARTDESAAGDHPIAANAIAAAAGRWSPAAAP
                     AAPEVTPPSGRNHPAPGFAAPLKGGCSGVADDAGFARFFARSVRRHRDPERAIVTITV
                     PLGDLKSGALRAIADLATRHASDEVRIARDQNLALPHARRADLRPLYEGLAAAGLDDG
                     TAGTALDVTSCPGADTCALGITSSKGVAAAVRRALLPLAGNGAAAALEGVTIKVSGCP
                     NSCGQHHIANIGLHGVCRTVNGKQVPAYQIHLGGSIADGTGRIGQALDKIPARRVPAA
                     VATLLKWYAGERGETETLPAFFDRQPKEKVRATLLPFATVAPIGADPVEDHEAAGAEE
                     PLEIDWGASASFSTDDLGTGECAGAGQDKADDPFDNARAELLQARLFLERGQTVDALA
                     NLNRSQYSVARVLLEALGRKPDSDYETTCELRARVIDRGLADEGWNEIHETVEELLKA
                     RRPEEDAVARLHARALAVLEASVPVHARLRRVAAAATAGAEVPA"
     gene            2651..3484
                     /locus_tag="VFD06_10755"
     CDS             2651..3484
                     /locus_tag="VFD06_10755"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZN04049.1"
                     /translation="MSQPHRGGALKTQTEMEESAALIESWKASEVLRWALDRFHPRLA
                     FASSFGAEDVVVIDLLSRLRRDARIVTLDTGRLHEETYDVMERVRRRYGMTIEVHAPE
                     REAVERLERDRGFFSFRDSIEARKECCKVRKVEPLRRALHGLEAWMTGLRRTQGVTRG
                     GVPKIEYDAAFGLIKLNPIADWSEQQVWDYIREHDVPYNALHDRAYPSIGCAPCTRAV
                     QPGEDARAGRWWWEQPELKECGLHEVRAEAGAKPAKTVGDDAVAIQAATAPAPSGGPR
                     R"
     gene            3572..4063
                     /locus_tag="VFD06_10760"
     CDS             3572..4063
                     /locus_tag="VFD06_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04050.1"
                     /translation="MDLDPKLDRAYRLLERGYERQRAGDLEAARRLYKASIEERPTAE
                     AHTYLGWTYSFQGKFDDAIEQCKTAILVDPDFGNPYNDIGSYLIQKGELDKAIPWLQM
                     AKKASRYEPRHFPYLNLFRVYLKQGRLDEAQREFEQARFIQEVLEMEAGVAPDEETAG
                     LTH"
     gene            4079..5272
                     /locus_tag="VFD06_10765"
     CDS             4079..5272
                     /locus_tag="VFD06_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04051.1"
                     /translation="MPPWSLTRRAFLCFGSLPVFAPLAVGPLAAIASRPAAAPARASD
                     ALDAALRLFQARAPESGSPGGRLSNHGPMVAEALETIGRPEAIEPFAARYLAGQEAAP
                     AAAAPFAASEWREGLGRDADFSRWLALFRREIDRSGWREATRLWAGRLAPGLAGQATH
                     GILRAGHAARALSRQETPARRDELAHGLAYWAASYREVLPGRPPVAGTLLPSQALART
                     PLLPAERRITRGLTVGRLAPLLDFPPFLQAADLVDWRGDASVYLNDLCRAFAGVYLAN
                     TDTSPIILLHAVTGPSAARLLLPYADAAGRQALLAHAWRASAGFYAVTALRLPAASAG
                     AAAEAPAAADAPAPMPREVIDGAVENGAVHAIKFAEACVREWNVAADPVLLRAAADGV
                     RRLNA"
     gene            complement(5269..6708)
                     /locus_tag="VFD06_10770"
     CDS             complement(5269..6708)
                     /locus_tag="VFD06_10770"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZN04052.1"
                     /translation="MASQHVGAGGEDLNLWNIVGRMFDKAAANLNIPEGLLQQIKACN
                     AVYYVQFPVKIGDRYEMFQGWRAEHSQHRKPTKGGLRYSTAVEQDEVMALAALMTYKC
                     AIVDVPFGGSKGGIKLDPKKYTRDQLEHITRRFTAELLRKNFIGPGINVPAPDLGTGE
                     QEMAWIADTYDALMPGQIDSLACVTGKPVTQGGIRGRREATGRGVQFGIREAFKHKAD
                     LKRCGLDGDLAGKRIAVQGFGNVGYHVARFLSEEDGCKVVGIGDVTGGLYDERGIDIQ
                     ALTEHRAKTGSISGFRGATSLKGSTEVLEVDCDILIPAALENQITMQNASRVKARIVA
                     EAANGPTTLGADETLLKRDVLVIPDIYLNAGGVTVSYFEWTKNLSHIRYGRMEKRLDE
                     MYREKLVSTIEQVTKAHVPDDLRRDLLHGADEVDLVNSGLEGSMQNAYREIRQALDQD
                     PRHGDLRTAAYAVAIAKVARTYMELGVFP"
     gene            6830..6904
                     /locus_tag="VFD06_10775"
     tRNA            6830..6904
                     /locus_tag="VFD06_10775"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6865..6867,aa:Ile,seq:cat)
     gene            6977..9412
                     /locus_tag="VFD06_10780"
     CDS             6977..9412
                     /locus_tag="VFD06_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04053.1"
                     /translation="MDTFLQDLKYAVRSLRARPAISAVAILALALGIGANSAIFSLVH
                     AVLLRPLPFADPDRIVMIWHEYKGMNLAKATISVPTYIEYRDHIEGFESVSVATNWSA
                     NLTGTGDPERLPGGRVSGNLFRTLGVVPALGRDFLPEEDAPGANRVVLLSDGLFKRRF
                     GADPSIVGKSLSLNGENHTVVGVLPAGFQFLQPVDVYRPAAFTPEESSPDNHGYEYLV
                     GVARVKRGVTMEQAQAQLKSLGAKLQKQFYPEGWGPYMVPVRDEIVGDLRPALLMLNA
                     AVACVLLIACANVANLLLARATARGKEIAVRVALGAGRKRIARLLLTESLLLGLLGGA
                     AGLLVARWGIQALQALAPTRLMQTVLGGRAVTINETVLVFTFGVSILTGLLFGLAPAL
                     RAARADLHDRLKEGARGESVGTHGHRLLGGFVVSQMAVAFVLLVGAGLLLRSFARLRE
                     VDPGFRAEKIMTMRLTLPSNRYDGDVAIGGFYGGLMERLRALPGVTAAAGVSNLPMAQ
                     NNASGSFEIEGSPTPDGQPGPHGDSHYVTQGYFEAMAIPVVEGRFFDTRDGREGTPSI
                     IVDDVLAKRYWPNGGALGHRLSKAGEGTDDKPVWRTVVGVVGHVKKYGLDGRVKEQYY
                     IPAEQKPMAAMSLVLKSAADPKNIVSAARAAIRSADADLPVFEVKTMDEVIDDTLMMR
                     RFVMSLIAGFAATALLLAAIGLYGVLAYSVSQRTHEIGVRMALGARARDVILMVVRRG
                     MLLAVAGLALGGLLAIGLTRLIQSQLFGVGAADPVTYALFGLALAGTALFACWLPARR
                     AALVPPTVALRYE"
     gene            complement(9419..>9706)
                     /locus_tag="VFD06_10785"
     CDS             complement(9419..>9706)
                     /locus_tag="VFD06_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04054.1"
                     /translation="EGTSFNIYDRADGAWHQIWVDSNGTRLDLVGGLRDGKMVLDGRD
                     RKGPRGGTVRDRITWTPRPDGTVRQHWEQSKDGGATWTTAFDGLYSRRSGT"
BASE COUNT         1342 a         3510 c         3424 g         1430 t
ORIGIN      
        1 ctggacaggt ccacaccggc cgccacgatc gagaacggca cgctccccgg cgcgcgcgtc
       61 cggcacgggc ggctcgagga gctcgaatcg ctcggcgccg gccggggcga cggccccctc
      121 ctggtggtcg tcggccggac cgcggcgctc gccggggatc tcgccggtcc gctcgccgcc
      181 ggggcgcgcc tcgcggcgga gaccggcgcc gaaactgcca ccgacaccgt cgcgccgcag
      241 gacgccgcag aaaccgccgc cattcccttc aggaggacgg gacgatgaac gaactcgatt
      301 cgaccagcct ccctcccgag accgccgccg agatcgcgaa catcgagtcc gagatcggcc
      361 gcttcctcgc cgggacgctc gcccccgagc gcttccgctc cttccgcctg gcgcacggca
      421 tctacggcca gcggcagccg ggagtgcaga tggtccgggt gaagatcccg accggggcgc
      481 tccacggcgc gcagctgcgc ctgctcgccg atctcgtcga cgacttcagc aacggctccg
      541 cccacctgac gacgcggcag gacgtgcagt tctactacgt ccgtctggag cgggtccccg
      601 acatgctgcg ccggctcgcc gccggcggcc tgacgacgcg cgaagcgtgc ggcaacagcg
      661 tgcgcaacgt caccgcctgc ccgctctccg gatccctgca ggacgaggcg ttcgacatcc
      721 gcccctacgc cctcgccacg tggcgctacc tggtgcgcaa tgccttctgc cagcagatgt
      781 cgcgcaagtt caagatcgcc ttctccgcct gcccggagga ctgcgccgcg accgccatcc
      841 acgacatcgg cgcgctcggc cgggtggtgt tccgggacgg cgtccagaag cagggcttcc
      901 gcgtcgtcgt cggaggcggg ctcgggccgt cgccgttcgt ggcgcaggtg ctgcgcgact
      961 tcgtcgcgcc cgaagatctc ctgccgacct tcaaggcgat cctcctggcg ttcgcggagc
     1021 aggggaaccg ccgcctgaag agcaaggccc ggctgaagtt cgtcctgcac cgcctcggca
     1081 tcgagcgctt ccgggcgctc gtggacgaga agctcgcggc cctttcgacg gcggagcgcg
     1141 acgaggcgga tctcgcccgg tggcttcagg acggcgacgc ggcggaggcc gcggcgcgga
     1201 cggacgagag cgccgccggg gatcacccca tcgcggccaa tgcgatcgca gcggccgcgg
     1261 ggaggtggag tccggccgcg gcgccggcgg cgccggaggt caccccgccc tccgggagga
     1321 accaccccgc gcccggtttc gcggccccgc tcaagggggg gtgcagcggc gtcgccgacg
     1381 acgccggctt cgcccgcttc ttcgcgcgct cggtgcggcg ccaccgggat ccggagcgcg
     1441 ccatcgtgac gatcacggtc ccgctcggag acctgaagtc cggggcgctg cgggccatcg
     1501 ccgacctcgc gacccgccac gcctcggacg aggtgcggat cgcgcgcgac cagaatctcg
     1561 ccctgccgca cgcccgccgc gccgatctgc gcccgctcta cgaagggctc gcggcagccg
     1621 gcctcgacga cggcaccgcc ggcacggcgc tcgacgtcac ctcctgcccc ggcgcggaca
     1681 cctgcgccct cggcatcacg tcttccaaag gggtggcggc ggcggtgcgc cgggcgctcc
     1741 tgccgctcgc cggcaacggc gcggctgcgg cgctcgaagg ggtcacgatc aaggtcagcg
     1801 gctgcccgaa ttcctgcggc cagcaccaca tcgccaacat cgggctgcac ggcgtctgcc
     1861 gcacggtcaa cggaaagcag gtccccgctt accagatcca cctgggaggg tcgatcgccg
     1921 acggcaccgg gcggatcggc caggcgctcg acaagatccc ggcgcggcgc gtccctgcgg
     1981 cggtggcgac gctcctcaag tggtacgccg gggagcgcgg cgagacggag acgctgccgg
     2041 ccttcttcga tcgccagccc aaggagaaag tgcgcgccac gctcctgccc ttcgcgacgg
     2101 tggccccgat cggcgccgat ccggtcgaag accacgaggc ggccggggcg gaggagccgc
     2161 tcgagatcga ctggggcgcc agcgcctcct tctccaccga cgacctgggg accggggagt
     2221 gcgccggcgc cggccaggac aaggcggacg acccgttcga caacgcgcgg gccgagctgc
     2281 tgcaggcacg cctgttcctg gagcgcggcc agaccgtcga cgccctcgcc aacctgaacc
     2341 gctcgcagta cagcgtcgcc cgcgtgctgc tcgaggcgct cgggcgcaaa ccggactcgg
     2401 actacgagac gacctgcgag ctgcgggcgc gcgtgatcga ccgcggcctc gcggacgagg
     2461 gatggaacga gatccacgag acggtcgagg agctcctgaa agcccgccgt ccggaggagg
     2521 acgcggtcgc ccgcctgcac gcgcgggcgc tcgcggtcct ggaagcgtcg gtgccggtgc
     2581 acgcccggct ccggagggtc gccgccgcag cgaccgcggg cgcggaagtt cctgcgtgag
     2641 ggatgggacc atgtcgcaac cacacagagg aggggccttg aagacgcaga cggagatgga
     2701 agagagcgca gcgctcatcg agtcgtggaa ggcgtcggag gtcctgcgct gggcgctgga
     2761 ccggttccat ccgcgactcg cgttcgcctc gtcgttcgga gccgaggacg tggtcgtgat
     2821 cgatctcctg tcgcggctgc ggcgcgacgc gcggatcgtc accctcgaca ccgggcgcct
     2881 gcacgaggag acctacgacg tgatggagcg cgtccgacgg cgctacggca tgacgatcga
     2941 ggtccatgcc ccggagcgcg aggcggtcga gcggctcgag cgcgatcgtg gcttcttctc
     3001 cttccgcgac agcatcgagg cccgcaagga gtgctgcaaa gtccgcaagg tcgagccgct
     3061 gcgccgggcg cttcacgggc tcgaggcgtg gatgaccggg ctgcgccgca cccagggggt
     3121 cacccgggga ggcgtgccga agatcgagta cgacgccgcc ttcggcctca tcaagctcaa
     3181 cccgatcgcc gactggagcg aacagcaggt gtgggactac atccgcgagc acgacgtgcc
     3241 gtacaacgcg ctgcacgatc gcgcctatcc ctcgatcggc tgcgctccgt gcacgcgcgc
     3301 cgtgcagccc ggggaggacg cccgcgccgg ccgctggtgg tgggagcagc cggaactcaa
     3361 ggagtgcggc ctgcacgagg tccgggcgga ggccggcgcc aagcccgcga agacggtcgg
     3421 cgacgacgcc gtcgccatcc aggccgccac ggcccccgct ccgtccggag gtccgaggcg
     3481 ctgacaccga cgctcgaagc cggatcgcga cgaggttgac gccggggtga acggccgcta
     3541 acatcgaccc ccgttcgggg gggttccaca gatggatctc gatcccaagc tggaccgcgc
     3601 ctaccggctc ctggagcgcg gctacgagcg acagcgcgcc ggcgacctcg aggcggctcg
     3661 gcgcctctac aaggcctcga tcgaagagcg gccgaccgcg gaggcgcaca cctacctcgg
     3721 ctggacgtat tcgttccagg gaaaattcga cgacgcgatc gagcagtgca agacggcgat
     3781 cctcgtcgat cccgacttcg gcaacccgta caacgacatc ggctcgtacc tcatccagaa
     3841 gggggagctc gacaaggcga ttccctggct gcagatggcg aagaaggcgt cgcgctacga
     3901 gccgcgccat ttcccctacc tgaacctgtt ccgcgtctat ctcaagcaag gacggctgga
     3961 cgaagcgcag cgcgagttcg agcaggcgcg cttcatccag gaggtgctgg agatggaagc
     4021 cggggtcgcc cccgacgagg aaaccgccgg cctgacgcat taaggggaag acgccaccgt
     4081 gcccccctgg agcctgacgc gccgcgcctt cctgtgcttc ggctcccttc cggtcttcgc
     4141 accgctcgct gtcggcccgc tcgcggccat cgcgtcccgg cccgccgcgg cgcctgcccg
     4201 cgccagcgac gcgctcgatg cggcgctgcg gctgttccag gcgcgcgccc ccgaatcggg
     4261 gtctccgggc gggcggctct cgaaccacgg gccgatggtg gcggaagcgc tcgagacgat
     4321 cggccgcccg gaggcgatcg agccgttcgc ggcccgctac ctcgcggggc aggaggcggc
     4381 ccccgccgcc gcggcgccgt tcgcggcgtc cgagtggcgg gaagggctcg gacgcgacgc
     4441 cgacttctcc cgctggctgg cgttgttccg gagggagatc gatcgttcag gctggcgcga
     4501 ggcgacgcgc ctgtgggccg gccgcctcgc ccccgggctc gccggccagg cgacgcacgg
     4561 catcctgcgc gccggccacg cggcccgcgc gctgtcgcgg caggagacgc cggcgcggcg
     4621 cgatgagctg gcgcacggcc tggcgtactg ggcggcgagc taccgcgaag tcctgccggg
     4681 ccggccgccg gtcgccggaa cgctcctgcc gtcgcaggcg ctggcgcgca cgccgctcct
     4741 gccggcggag cggcgcatca cccgcgggct gacggtcggc cggctggcgc cgctcctgga
     4801 tttcccgccg tttctccagg cggcggatct cgtggactgg cgcggcgacg cgtcggtcta
     4861 tctcaacgat ctctgccgcg ccttcgccgg cgtctatctc gccaacaccg acacctcgcc
     4921 gatcatcctg ctgcacgcgg tcaccggtcc ctcggcggcg cggctgctgc tgccgtacgc
     4981 ggacgcggcc ggccggcagg cgctcctggc gcacgcgtgg cgcgcctcgg ccggcttcta
     5041 cgccgtcacc gccctgcggc tccccgccgc gtccgcgggc gccgcggcgg aggcgcccgc
     5101 cgcggcggat gccccggcgc cgatgccgcg cgaggtgatc gacggcgcgg tcgagaacgg
     5161 cgccgtgcat gccatcaagt tcgcggaagc ctgcgtgcgg gagtggaacg tcgccgccga
     5221 tccggtgctg ctgcgcgcgg cggcggacgg cgtccggcgc ctgaacgcct aagggaagac
     5281 gccgagctcc atgtaggtgc gcgccacctt ggcgatggcg acggcgtagg cggcggtgcg
     5341 caggtcgccg tggcgcgggt cctgatcgag cgcctggcgg atctcccggt aggcgttctg
     5401 catcgatccc tcgagcccgg agttgaccag gtccacctcg tccgcgccgt gcagcaggtc
     5461 gcggcgcagg tcgtcgggga cgtgcgcctt ggtgacctgc tcgatcgtcg agaccagctt
     5521 ctcgcggtac atctcgtcga ggcgcttctc catccgcccg tagcggatgt gcgacaggtt
     5581 cttcgtccac tcgaagtagg agacggtgac gccccccgcg ttcaggtaga tgtccgggat
     5641 gacgaggacg tcgcgcttca ggagcgtctc gtcggcgccg agcgtcgtcg gaccgttcgc
     5701 cgcctcggcg acgatgcgcg ccttgacgcg cgaggcgttc tgcatcgtga tctggttctc
     5761 gagcgccgcc gggatcagga tgtcgcagtc gacctccagc acctcggtcg atcccttgag
     5821 cgacgtcgcc cccctgaagc cggagatgct gccggtcttc gcccggtgct cggtgagggc
     5881 ctggatgtcg atgccgcgct cgtcgtagag cccgccggtg acgtcgccga tcccgaccac
     5941 cttgcagccg tcctcctcgg agaggaagcg ggcgacgtgg tagccgacat tgccgaaccc
     6001 ctgcacggcg atccgcttgc cggcgagatc gccgtcgagg ccgcatctct tgaggtccgc
     6061 cttgtgcttg aacgcctcgc ggatgccgaa ctgcacgccc cgtccggtcg cctcgcgccg
     6121 gccgcggatc cccccctggg tgaccggctt gccggtgacg caggcgagcg agtcgatctg
     6181 ccccggcatc agggcgtcgt aggtgtcggc gatccaggcc atctcctgct cgccggtgcc
     6241 gaggtcgggg gcggggacgt tgatcccggg gccgatgaag ttcttgcgca agagctccgc
     6301 ggtgaagcgg cgggtgatgt gctccagctg atcgcgggtg tacttcttcg ggtcgagctt
     6361 gatccccccc ttggagccgc cgaaggggac gtcgacgatg gcgcacttgt aggtcatcag
     6421 cgccgcgagc gccatcacct cgtcctgctc caccgcggtc gagtagcgca gccccccctt
     6481 ggtcggcttg cggtgctgcg agtgctcggc ccgccacccc tggaacatct cgtagcggtc
     6541 gccgatcttc accgggaact ggacgtagta gaccgcgttg caggccttga tctgctgcaa
     6601 cagcccctcg gggatgttga ggttcgccgc cgccttgtcg aacatgcggc cgacgatgtt
     6661 ccagagattg aggtcctcgc cacccgctcc gacgtgctga ctcgccatcg ttcgctcctc
     6721 gcctcgccct tgatgtgtgc ggaacgcgga atcctcgcac acccccccgc ttcggctcaa
     6781 gggacctggc ttcagctcga tccgatgcgg ggtgcatata atccggcgag ggcccatagc
     6841 tcaggtggtc aagagcagct gactcataat cagttggtcc ctggttcgaa tccaggtggg
     6901 cccacctcga gatccggcgc ctgtccgtgg aaccgacgtc cggcgcctgc gttcaagaac
     6961 ccggaggaca gcgccggtgg acaccttcct gcaggacctg aaatacgccg tccgctcgct
     7021 ccgggcgcgg ccggcgatct cggcggtggc gatcctggcg ctggcgctcg gcatcggcgc
     7081 gaacagcgcc atcttcagcc tggtgcacgc cgtcctcctg cggccgctgc cgttcgccga
     7141 cccggaccgc atcgtgatga tctggcacga atacaagggg atgaacctcg ccaaggcgac
     7201 gatctcggtg ccgacctaca tcgagtaccg cgatcacatc gaggggttcg agagcgtctc
     7261 ggtggcgacg aactggagcg ccaacctcac cgggaccggc gatccggaga ggctccccgg
     7321 cgggcgggtc agcggaaatc tcttccgcac gctcggtgtc gtgcccgccc tcgggcgcga
     7381 cttcctgccg gaggaggacg ccccgggcgc caaccgcgtc gtcctcctgt cggacggcct
     7441 cttcaagcgg cgcttcgggg ccgacccgtc gatcgtcggg aagtcgctgt cactcaatgg
     7501 cgagaaccac acggtcgtcg gtgtgctccc ggccgggttc cagttcctgc agccggtgga
     7561 cgtctaccgc cccgcggcct tcacccccga ggagtcctca cccgacaacc acggctacga
     7621 gtacctcgtc ggcgtggcgc gcgtgaagcg cggcgtcacg atggagcagg cgcaggcgca
     7681 gctcaagtcg ctgggcgcga agctccagaa gcagttctac cccgaggggt ggggtcctta
     7741 catggtgccg gtgcgggacg agatcgtcgg cgacctgcgg ccggcgctgc tgatgctcaa
     7801 cgccgcggtg gcgtgcgtgc tcctcatcgc ctgcgccaac gtcgcgaacc tgctgctcgc
     7861 ccgcgccacg gcgcgcggca aggagatcgc ggtgcgcgtc gcgctcggcg ccgggcggaa
     7921 gcgaatcgcg cggctcctcc tgacagagag cctcctgctc ggtctgctcg gcggcgcggc
     7981 cgggcttctc gtggcgcgct gggggatcca ggcgctccag gcgctggcac cgacgcgtct
     8041 gatgcagacg gtgctcggcg gacgcgcggt gacgatcaac gagacggtcc tcgtcttcac
     8101 cttcggggtg tcgatcctca ccgggctcct gttcggcctt gcccccgccc tgcgggcggc
     8161 gcgcgccgac ctccatgacc gcctcaagga gggggcgcgt ggcgagtcgg tcgggacgca
     8221 cggccatcgc ctgctcggcg gcttcgtcgt gtcgcagatg gccgtggcct tcgtgctcct
     8281 cgtcggggcc gggctgctgc tgcgctcctt cgcacggctg cgcgaggtgg atcccggctt
     8341 ccgcgcggag aaaatcatga cgatgcgcct cacccttcct tcgaaccgct acgacgggga
     8401 cgtcgccatc ggtggtttct acggagggct gatggagcgg ctgcgggcgc tgcccggcgt
     8461 gaccgccgcc gccggcgtct ccaatctccc gatggcgcag aacaacgcct ccggatcgtt
     8521 cgagatcgaa gggtcgccga ccccggacgg gcagccgggg ccgcacggcg actcgcacta
     8581 cgtgacgcag gggtatttcg aggcgatggc gatcccggtc gtcgagggac gcttcttcga
     8641 cacgcgcgac gggcgcgaag gcacgccgtc gatcatcgtc gacgacgtcc tggcgaagcg
     8701 ctactggccc aacggcggag cgctcgggca ccgcctctcg aaggcggggg aggggaccga
     8761 cgacaagccc gtctggcgca ccgtcgtcgg cgtcgtcggc cacgtcaaga agtacggcct
     8821 cgacggccgc gtcaaggagc agtactacat cccggccgag cagaagccga tggccgccat
     8881 gtcgctggtg ctcaagagcg ccgccgatcc gaagaacatc gtctccgcgg cgcgcgccgc
     8941 catccgctcg gcggacgccg atctgccggt gttcgaggtc aagacgatgg acgaggtgat
     9001 cgatgacacg ctcatgatgc ggcgtttcgt gatgtcgctc atcgccggtt tcgccgccac
     9061 cgccctgctg ctcgccgcga tcgggctgta cggcgtgctc gcgtactccg tgtcgcagcg
     9121 cacccacgag atcggcgtac gcatggcgct cggggcgcgc gcgcgcgacg tcatcctgat
     9181 ggtggtgcgg cgcggcatgc tcctcgcggt cgcggggctg gccctcgggg gactcctggc
     9241 gatcgggctg acccggctca tccagagcca gctcttcggc gttggcgccg ccgatccggt
     9301 gacgtacgcc ctcttcgggc tggcgctcgc cgggaccgcg ctcttcgcct gctggctgcc
     9361 ggcgcggcgc gccgcgctcg tcccgccgac cgtcgcgctg cgctacgagt agcccggcct
     9421 aggttccgga gcgccgcgaa tacaggccgt cgaacgccgt cgtccaggtg gcgccgccgt
     9481 ctttggactg ctcccagtgc tgccgcaccg tgccgtcggg tcgcggcgtc caggtgatcc
     9541 ggtcgcgcac cgtcccgccg cgcggcccct tccgatccct tccgtcgagg accatcttcc
     9601 cgtcgcgcag gccgcccaca aggtcgaggc gcgtgccgtt gctgtccacc cagatctggt
     9661 gccaggcgcc gtcggcccgg tcgtagatgt tgaacgaggt cccctc
//
LOCUS       DASRIQ010000441         6869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3045748, whole genome shotgun sequence.
ACCESSION   DASRIQ010000441 DASRIQ010000000
VERSION     DASRIQ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3045748"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2170
                     /locus_tag="VFD06_10790"
     CDS             <1..2170
                     /locus_tag="VFD06_10790"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZN04055.1"
                     /translation="PVMTLAGICFAASLALTLVVEPQARYMQRRLYSQQLYSSDLRRE
                     IKPRVFFEQVPGLLMYADNVYQDGDFLSRVFLHQTDPEGREVVTLARRAQIEYDRKGG
                     VVEFLLEAGTNHTMTPGEPESYQISHFDRQRVLRGPDDTFRMKMGILSRPAPRNYSEQ
                     SLAELNTSIVQAGSLMAGTTQDRVIGSIRAEIHARWSLPFACITLAFLGLPLGIANRR
                     GGRGSGFTLSLCVVIAYWALSSSGQQMVREGRLSPWIGIWGPHAILIALGCVLFLARE
                     RSEGLNPAVLIPPRLMHLFRIRRAPRTADATGTFAQRPLSSKIWRIAVGLMTFILGLG
                     AVVLTPFLAVGLLLLVSIVSFSTTLDRDILKRFLLILVGCILSFFTLFMVYEFVQILD
                     DLVERHQPATLAFAYLAYRIPWILSQVTPMSCLMAALLTYGLMSRFNEVTAVKAAGTS
                     IYRLSVPVLLATLVTSVLAYVNYDYVVPFSNQEYVRLKDTIRGRSPRSYAIGDRRWVF
                     GEGGRLFNFRNYVAPALPALPGSAGGTFEGFSVYLLDPSSFDMRGRLYARTAVRSEAG
                     WVLRDGWVREFGQNGEVFERFSQKAFEFPENEGFFVKEWKKPEQMNFTELRRLIRDLR
                     LRGYDAQELMVDLYSKTSFPLVPLTLVIIGLPFCFRLGRHGSLYGVAIAILLAGVYFL
                     LFSATSALGATGIMPAFLAAWAPNILFIGAGSYLLLRTTT"
     gene            complement(2178..3035)
                     /locus_tag="VFD06_10795"
     CDS             complement(2178..3035)
                     /locus_tag="VFD06_10795"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04056.1"
                     /translation="MIRPAVALVAAVLAQAAVRESVPLSLYQLLARAPIVIHGRITRG
                     AGRLAEVKVLENFRGTAPAETIRLDFRDLNLDSTIKDQLAFLDGEEYVLFLEHPNWRK
                     PTKKKENILGLYHGREGFRRLPEEGAGEQIEAIRQLAAVMALPSGEQADALRQRAQQH
                     NATLQINALEELLRLQEGGREDLDWLQRIVRDPDPGVRSKAAALMARAFAEVPAETAE
                     QEREALESVRERAHGDPDAKVRAASTRALGSWPNRDQVVPDLQAIGSGDASQDVRYEA
                     RRLLFLWGK"
     gene            complement(3032..6070)
                     /locus_tag="VFD06_10800"
     CDS             complement(3032..6070)
                     /locus_tag="VFD06_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04057.1"
                     /translation="MGIRLSRRRRAAAGLAVLTAAVWLLCGLRRVPEEEGRAAVLDSP
                     LGLVEPSRAAPGWHLVPPGLLRLSFYPVRSTTLTISAGAGSDPLRTRDGSLVDAEILL
                     RYRVDDDRLLDVHRRLGPRFERETVVRWAGEALRQTVGGAAYSEISGRRTGGVQDEIG
                     QLLGERLRASGMILQACEVGRIRLRPDASGEPTAAVSLPAGRVLLVGLDGADWNIVDP
                     LRQAGRMPHLDRLVRGGTRARLKTIAPMLSPVLWTSIATGVVPQRHGILDFVTGTAQD
                     GGQVPVSARQRRVKAFWNILSERGLSVGVAGWWATWPAEQVNGFIVSDRVAYQLLGVR
                     PVEERNRDGKVFPASGDERVIAATVAPESIGPGDLKAFLRLPADPAALPEKESRLVDD
                     LRTVVASGRTYTAATLALAAGRPPDLTAVYFEGTDTVAHLFMPYAPPPLEGIEPGAQR
                     RFGKAVDAYYEEADRLLGQLIDGLQPATVVVLSDHGFRTGDNRPRTESRPGYGAAADW
                     HRHYGIAVFHGPAFRRGADLEEVSLLDIAPTLLRLVGLPVGEDMDGRPIAAAFDPAWQ
                     AAHPEQYIASWERSEATPVVVAAAPAREIRAESGPAGAAGAASNPNRAPDGSAAPAAA
                     ALAQGAPAGAAGKGSPTDAAEDAASADDAGDAERVAKLQSLGYVASGDSPNAHNNRGA
                     TLLFQGRAEEALREFEQAIAAGEDRTIARMNLARARIELKDYDGALRDIEEHTKVQPR
                     SRDAEYLRGQIAERRRRPEEAERHYRAALLIDPNFTEARNDLGLLQERQGRHDEALQE
                     FRRVVEIDPEFGEAYNNIGIVLRGRGRTDEAADAFRRAIKASPEFAGAYSNLALLLED
                     RKEYGEAEALLRQALKRAPSDADVRSNLGALLHLVGRDEEAVRELERATSLDPRSASA
                     FNNLGTALGRLGRSAEEMAAYRRALSLDPSSAEAHYNLGRALLRAGQAAEGRMEMEAA
                     RGLRPSDPDLARRIEEALQGMSETPDGAAPSGPAQQGGSR"
     gene            complement(6117..>6869)
                     /locus_tag="VFD06_10805"
     CDS             complement(6117..>6869)
                     /locus_tag="VFD06_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZN04058.1"
                     /translation="PIIAVNDAMTKHFFDNRYGTGQSTIDGIIRATNLLIAGLKVVVV
                     GYGWCGRGVAQRAAGLGGQVIVAEVEPVKALEAVMDGFQVMPMKEAVKVGDIFLTLTG
                     NKHVLRGEHFQAMKDGAVIANSGHFNVEIDIPALEALAGGKKERIRDYVDQYRMKDGR
                     KLNLLGEGRLINLAAAEGHPAAVMDMSFANQALSVEYLAKEGKTLAKKVHPVPMVIDR
                     EIARLKLASMGVTIDTLTDEQKTYLSSWSEGT"
BASE COUNT          908 a         2494 c         2353 g         1114 t
ORIGIN      
        1 gccggtgatg actctggccg ggatttgctt cgcggccagc ctggcgctga ccctcgtcgt
       61 cgagccccag gcccgctaca tgcagcgacg cctgtacagt cagcagctct actcctcgga
      121 cctgcggcgg gagatcaagc cgcgcgtctt cttcgagcag gttcccgggc tcctcatgta
      181 cgccgacaac gtctaccagg acggcgactt cctgagccgg gtcttcctgc accagaccga
      241 ccccgagggg cgtgaggtcg tcacgctcgc ccgccgcgcc cagatcgagt acgaccgcaa
      301 gggcggcgtc gtggagttcc tcctcgaggc cgggacgaac cacaccatga cgcccggcga
      361 gcccgagagc taccagatca gccacttcga ccggcagcgc gtcctccgcg gaccggacga
      421 cacgttccgg atgaagatgg ggatcctgtc gcgcccggcg ccgcgcaact actccgagca
      481 gagcctcgcg gagctcaaca cctcgatcgt ccaggccgga tcgctcatgg ccggcacgac
      541 ccaggaccgt gtgatcggat ccattcgggc ggagatccac gctcgctggt cgctcccctt
      601 cgcctgcatc accctcgcgt tcctcggcct gccgctcggg atcgcgaacc gccgtggcgg
      661 gaggggctcc gggttcacgc tcagcctctg cgtcgtcatc gcgtactggg cgctgtccag
      721 ttccggccag cagatggtgc gcgaggggcg tctctccccc tggatcggaa tctggggacc
      781 gcacgcgatc ctcatcgcgc tcggatgcgt cctctttctt gcgcgcgagc gcagcgaggg
      841 gctcaatccc gccgtgctga tcccgccgcg cctgatgcac ctgttccgga ttcgccgggc
      901 accgcgcacg gccgatgcga ccgggacgtt cgcgcagcgg ccgctgagct cgaagatctg
      961 gcgcatcgcg gtggggctca tgaccttcat cctcggcttg ggggccgtgg tgctgacgcc
     1021 gttcctggcg gtcggcctgc tcctgctggt ctcgatcgtt tcgttcagca cgacgctcga
     1081 tcgcgacatc ctgaagaggt tcctgctgat cctcgtcggc tgcatcctgt cgttcttcac
     1141 cctcttcatg gtctacgagt tcgtgcagat cctcgacgac ctggtggagc ggcaccagcc
     1201 cgccacgctc gcgttcgcct acctcgcgta ccggatcccg tggatcctgt cgcaggtgac
     1261 gccgatgtcc tgcctgatgg cggccctgct cacctacggt ctgatgtccc gcttcaacga
     1321 ggtcacggcg gtcaaggcgg ccgggacgag catctaccgt ctctcggtgc cggtcctcct
     1381 cgccacgctg gtgaccagcg tcctggcgta cgtcaactac gactacgtcg tgccgttctc
     1441 gaaccaggag tacgtccgcc tcaaggacac catccgcggc cgatccccgc gttcgtacgc
     1501 catcggcgac cggcgatggg tgttcggtga aggggggcgg ctcttcaact tccgcaacta
     1561 cgtcgccccg gctctcccgg cgctccccgg ctcggcgggc ggcaccttcg aggggttctc
     1621 cgtgtacctc ctggaccctt cgtcgttcga catgcgcggg cgcctctacg cccggaccgc
     1681 ggtgcgcagc gaggccgggt gggtgctgcg ggatgggtgg gtccgggagt tcgggcagaa
     1741 cggtgaggtc ttcgagcggt tcagccagaa ggcgttcgag ttcccggaga acgaggggtt
     1801 cttcgtcaag gaatggaaga agccagagca gatgaacttc accgagctcc ggcggctgat
     1861 ccgcgacctg cgtctgcgcg gctacgacgc gcaggagctg atggtcgatc tctactcgaa
     1921 gacgtcgttc ccgctcgtcc cgctcaccct cgtcatcatc gggctcccct tctgcttccg
     1981 tctcgggaga cacgggtcac tgtacggggt cgcgatcgcg atcctgctcg ctggggtcta
     2041 cttcctgctg ttctccgcga cgagcgccct cggtgcgacc gggatcatgc cggcgttcct
     2101 ggcggcctgg gcgccgaaca tcctgttcat cggagcggga tcctatctgc tgctgcggac
     2161 cacgacctga gaacggttca cttcccccag agaaagagca ggcgtcgcgc ttcgtagcgg
     2221 acatcctgcg aggcatcgcc ggatccaatc gcctgcaggt cggggacgac ctggtcccgg
     2281 ttgggccagg agcccagggc gcgggtcgag gccgcccgca ccttggcgtc gggatcgccg
     2341 tgggcccgtt cccgcaccga ctccagagcc tcgcgttcct gctccgcggt ctcggccggc
     2401 acctccgcga aggcacgcgc catgagcgcg gccgccttcg accggacccc cggatccggg
     2461 tcgcggacga tccgctggag ccagtcgagg tcctcccggc ccccctcctg gagccgcagc
     2521 agctcctcga gagcgttgat ctggagcgtc gcgttgtgct gctgtgcccg ctgccgaagg
     2581 gcgtcggcct gctcgccgga ggggagcgcc atcaccgcgg cgagctggcg gatcgcctcg
     2641 atctgctctc ccgccccctc ctccgggagc ctccggaacc cctcccgtcc gtggtagagc
     2701 ccgaggatgt tctccttctt cttcgtcggc ttgcgccagt tcgggtgctc gaggaagagg
     2761 acgtactcct cgccgtcgag gaaggccagc tggtccttga tggtgctgtc caggttcaga
     2821 tcgcggaagt cgaggcggat cgtctccgct ggcgccgtgc cgcggaagtt ttctaaaact
     2881 ttcacctcag ccaggcgacc agcgccgcgg gtgatgcgcc cgtggatgac aatcggggcg
     2941 cgcgccagga gctggtagag gctgagcggc accgattccc ggaccgccgc ctgggccagg
     3001 acggcggcga ccagggcgac ggcggggcgg atcatcgcga cccgccctgc tgcgccggtc
     3061 cggacggggc ggccccgtcg ggggtctcgc tcatcccctg cagtgcctct tcgatgcgcc
     3121 gggcgaggtc cgggtcgctc gggcgcaggc cccgcgccgc ctccatctcc atccgcccct
     3181 cggcggcctg cccggcgcgc aggagcgccc gcccgaggtt gtagtgcgct tccgccgagg
     3241 aggggtcgag ggagagagcc cgtcggtacg ccgccatctc ctccgccgag cgcccgagcc
     3301 ggccgagggc ggtcccgagg ttgttgaagg ccgaggccga tcgcgggtcg agactcgttg
     3361 cgcgctccag ctcccgcacc gcctcctcgt cgcgaccgac gaggtgcagc agcgccccga
     3421 ggttgctgcg gacatcggcg tccgaggggg cgcgcttcaa cgcctggcgc aggagcgcct
     3481 ccgcctcgcc gtactccttc cggtcctcga gcagcagggc gaggttgctg taagcgcccg
     3541 cgaactccgg gctcgccttg atggcgcgac ggaacgcgtc ggccgcctcg tccgtgcggc
     3601 cgcgcccccg gaggacgatg ccgatgttgt tgtacgcctc gccgaactcc gggtcgatct
     3661 cgacgacgcg gcggaattcc tgcagcgcct cgtcgtggcg tccctggcgc tcctggagca
     3721 gccccaggtc gttgcgcgcc tcggtgaagt tgggatcgat gaggagggca gcgcggtagt
     3781 ggcgctccgc ctcctccggc cgccggcggc gctcggcgat ctgcccgcgc aggtactccg
     3841 cgtcgcgcga gcgcggctgc accttcgtgt gctcttcgat gtccctgagc gcgccgtcgt
     3901 agtccttcag ctcgatgcgg gcgcgcgcca ggttcatgcg ggcgatggtc cgatcctccc
     3961 cggccgcgat cgcctgctcg aactcccgga gcgcctcctc cgcccgcccc tggaagagga
     4021 gggtcgcgcc gcggttgttg tgggcgttcg ggctgtcgcc cgaggcgacg tagccgagcg
     4081 attgcagctt cgccacccgc tcggcgtcgc ccgcgtcatc ggccgacgcg gcatcctccg
     4141 cggcgtcggt cgggctcccc ttgccggcgg cgccagccgg cgcgccttgc gcgagtgcgg
     4201 cggcggccgg cgcggcgctc ccatccgggg cgcggttcgg gttcgacgcg gcgccggcgg
     4261 cgcccgccgg gccggactcc gcgcgaatct cccgggcggg cgcggccgcg acgacgaccg
     4321 gcgtcgcctc gctcctctcc cacgaggcga tgtactgctc gggatgggcc gcctgccagg
     4381 cgggatcgaa ggcggcggcg atcggccggc cgtccatgtc ctcccccacc ggcaggccga
     4441 cgaggcgcag gagcgtcggg gcgatgtcga ggagggagac ctcctcgaga tccgccccgc
     4501 ggcggaaggc cggtccatgg aacacggcga tgccgtagtg ccggtgccag tcggcggccg
     4561 ccccgtaccc cggacgggat tccgtgcgcg gccggttgtc gccggtgcgg aagccgtggt
     4621 cggagaggac cacgaccgtc gccggctgga ggccgtcgat gagttgcccg agcagccgat
     4681 cggcctcctc gtaataggcg tccaccgcct tcccgaaacg gcgctgcgca cccggctcga
     4741 tcccctcgag cggcggcggc gcgtacggca tgaacaggtg cgccaccgtg tcggtcccct
     4801 cgaagtacac cgcggtgagg tccgggggcc tcccggcggc tagggcgagg gtcgccgccg
     4861 tgtatgttcg acccgaggcg acgacggtgc gcaggtcatc caccagccgg ctttcctttt
     4921 cgggcagcgc cgccgggtcg gccggcagcc tcaggaacgc tttcagatct ccgggaccga
     4981 tcgattccgg cgccacggtc gcggcgatca cccgctcgtc ccccgacgcc gggaagacct
     5041 tgccgtcgcg gttacgctcc tccaccggcc gcacgccgag gagctggtac gccacccggt
     5101 ccgaaacgat gaagccgttg acttgctccg ccggccatgt ggcccaccac ccggcgaccc
     5161 cgaccgaaag gccgcgctcg ctcaggatgt tccagaacgc cttcacgcgc cgctgccggg
     5221 cgctgaccgg gacctgtccg ccgtcctgcg cggttccggt gacgaagtcg aggatcccgt
     5281 ggcgctgcgg cacgaccccg gtggcgatgc tcgtccagag caccggcgac agcatcggcg
     5341 cgatcgtctt cagccgcgcc cgcgtcccgc cgcggaccag acggtcgagg tgcggcatgc
     5401 gtccggcctg ccgcaacgga tcgacgatgt tccagtcggc gccgtcgagc ccgacgagca
     5461 gaacccggcc ggccggcagg gagacggccg ccgtcggctc tcccgaggcg tcgggccgca
     5521 gccggatccg ccccacctcg cacgcctgca ggatcatccc cgaggcgcgc agccgctcgc
     5581 cgagcagctg cccgatctcg tcctggacgc cgccggtgcg gcggccggag atctccgaat
     5641 aggcggcgcc gcccacggtc tgccgcagtg cctcgccggc ccagcgcacg accgtctcgc
     5701 gctcgaagcg cggcccgagg cgtcggtgca cgtcgagcag ccggtcgtcg tccacccgat
     5761 agcgcagcag gatctcggcg tcgacgaggc tcccgtcgcg cgtgcggagg ggatccgacc
     5821 ccgctccggc ggagatcgtc agggtggtcg atcggaccgg gtagaaggag agcctgagga
     5881 gccccggcgg gacgaggtgc cacccggggg cggcacgtga cggttcgacg agccccaggg
     5941 gggaatcgag gaccgccgcc ctcccctcct cctccggaac gcggcgcagc ccgcacagaa
     6001 gccatacggc cgccgtcagg acggccagtc cggcggcggc ccgccggcgt cgagagagtc
     6061 ggatccccat ggggcgcggc ggagaagggg ctccccctcc ccgccgcggg gagatcttag
     6121 gtcccttcgg accaggagga caggtaggtc ttctgctcgt cggtcagggt gtcgatggtc
     6181 acccccatcg acgccagctt caggcgggcg atctcccggt cgatcaccat cgggaccggg
     6241 tggaccttct tcgccagcgt cttgccctcc ttggccaggt actcgaccga aagggcctgg
     6301 ttggcgaacg acatgtccat gacggcggcc gggtgtccct cggccgcggc gaggttgatg
     6361 aggcgtccct cgccgagcag gttgagcttc cggccgtcct tcatccggta ctggtccacg
     6421 taatcgcgga tccgctcctt ctttccaccc gccagcgcct cgagcgccgg gatgtcgatc
     6481 tcgacgttga aatggccgga gttggcgatc accgcgccgt ccttcatcgc ctggaagtgc
     6541 tcgccgcgca ggacgtgctt gttcccggtg agagtcagga agatgtcacc gaccttcacg
     6601 gcctccttca tcggcatcac ctggaagccg tccatcaccg cctcgagcgc cttcaccggc
     6661 tcgacctcgg cgacgatcac ctgcccgccg agcccggcgg cacgctgcgc cacgccgcgc
     6721 ccgcaccagc cgtagccgac cacgacgacc ttgagcccgg cgatcaggag gttggtggcc
     6781 cggatgatcc cgtcgatggt gctctggccg gtgccgtagc ggttgtcgaa gaaatgcttc
     6841 gtcatcgcgt cgttgaccgc gatgatcgg
//
LOCUS       DASRIQ010000442         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3573957, whole genome shotgun sequence.
ACCESSION   DASRIQ010000442 DASRIQ010000000
VERSION     DASRIQ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3573957"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFD06_10810"
     CDS             complement(<1..741)
                     /locus_tag="VFD06_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04059.1"
                     /translation="MGLNPRASLEPVLEWRRRAGRAARRLRDRERTALPDENDLLRAF
                     GGRHGRIDEALSELAGGGVAIVAAAGQGEMRAAYAARPEWRDRLFEAASRILEHRFDL
                     LGSGDRGLGARLPWHADFKSGHAWNRDVPSEELFAQVAKEFGRGRDVKVPWELSRFQH
                     APLLAQAAWLAPDRRYYDEFRSQVEDWITANPVGRGVNWGCTMDVALRAASLATAATL
                     FRDELRADSGFASLLWRALLAHGRFVVTH"
     gene            complement(732..2363)
                     /locus_tag="VFD06_10815"
     CDS             complement(732..2363)
                     /locus_tag="VFD06_10815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN04060.1"
                     /translation="MSALTSPSAAVLLLVSDPDKVEPLLPGLLPGRSLRTLRRESLGG
                     GPVGLARRLGALGVDEIVLLTDDLDFHERLWRLQAIGALPLAPKRYLLDLRGRRLVLS
                     VGRFAVRDLPMLVVGLLGSGTVLWRTRRQLDRLLQAERHRPRPVHGRRVVFLRSDLWS
                     GVPAGGSVAHTSGVASGFAALGADLSFISTSAPALVDPAKHPAHLVPPSRRYNIFREV
                     PYFAHAIRFLNAARRRLSDAPADLLYQRFDPANWAGVALARERSIPLVLEYNGSEVWI
                     ADHWDRPFKHRDLFVKAETANLRHADLIVVVSEVLRQGLADRGIEPERLVVRPNAVDT
                     DRYRPDLDGGPVRRRLELGEGPVVGFVGTFGIWHGAPVLARAARRVLRERPHARFLFV
                     GDGRDRRECEAILADERASVRFTGLVPQDEGPGHLAAMDILAAPHVDNPDGTRFFGSP
                     TKLFEYMAMGRGIVASRLEQIGDVLEDGATALLVPPGDDAALARAVVRLVDDAALRAR
                     LGAAARERALERHTWTANVRGVVERLRDRGLLLWD"
     gene            complement(2360..>2897)
                     /locus_tag="VFD06_10820"
     CDS             complement(2360..>2897)
                     /locus_tag="VFD06_10820"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HZN04061.1"
                     /translation="VQIADTLLLLPVTVGMILLPRVAGERSERQEEVTARVLRHTAMM
                     LAGLCLAAGILVGPMVRLLYGESFSGAILATRLLLPGVLALGLNGVLMNHFGGRGMPA
                     ITAVAPAAGLLLNVALNVAVIPRFGIAGAALTSSLAYGLMLLLSLAAFLRGGRIGLMK
                     SLVVSPDDLRGLLGARQA"
BASE COUNT          457 a         1077 c         1038 g          325 t
ORIGIN      
        1 gtgggtgacg acgaagcggc cgtgcgccag aagggcccgc cacagcaggg acgcgaagcc
       61 ggaatccgcg cgcagctcgt cgcggaacag agtcgcggcc gtcgcgagcg acgcggcgcg
      121 cagcgcgacg tccatggtgc agccccagtt cacgccccga ccgaccgggt tggccgtaat
      181 ccagtcctcg acctgcgagc ggaactcgtc gtagtagcgc cggtccgggg cgagccaggc
      241 ggcctgcgcc agaagcggcg cgtgctggaa gcgggacagc tcccacggaa ccttcacgtc
      301 gcggccacgc ccaaactcct tcgccacctg ggcgaacagc tcctccgacg gaacatcccg
      361 attccaggca tggccgctct tgaagtcggc gtgccacggc aggcgcgccc cgagcccgcg
      421 atcccccgac ccgaggagat cgaagcggtg ctcgaggatc ctcgacgccg cctcgaacag
      481 gcgatcgcgc cattccgggc gggcggcgta ggcggcgcgc atctccccct gtccggcggc
      541 ggcgacgatc gccacccccc ctccggcgag ttccgacagg gcctcgtcga tgcggccgtg
      601 ccggccgccg aaggcgcgca gcagatcgtt ctcgtccggc agcgccgtcc gctcccggtc
      661 gcgcagccgg cgcgcggcac gcccggcgcg gcggcgccat tccaggaccg gctcgagaga
      721 ggcccgcggg ttcagtccca cagtaagagc ccccgatcgc gcagcctttc gacgacaccg
      781 cggacgttcg cggtccaggt gtgacgctcg agcgcccgct cccgcgccgc cgccccgagc
      841 cgtgctcgga gcgctgcgtc gtccacgagg cggacgacgg cccgggccag ggccgcgtcg
      901 tctccgggcg ggaccagaag cgcggtcgcg ccgtcctcga ggacgtcgcc gatctgctcc
      961 aggcgactgg ccacgatccc ccgccccatc gccatgtact cgaacagctt ggtgggggat
     1021 ccgaagaagc gcgtgccgtc cggattgtcc acgtgcggcg ccgccaggat gtccatggcc
     1081 gccaggtgac ccggaccctc gtcctgaggg acgagcccgg tgaagcggac cgacgcgcgc
     1141 tcgtccgcga ggatcgcctc gcactccctg cggtcgcgcc catcgccgac gaacaggaag
     1201 cgggcgtgcg gccgctcgcg caggacccgc cgcgcggcgc gggcgaggac cggcgcgccg
     1261 tgccagatcc cgaaggtgcc gacgaacccg accacgggtc cctctcccag ctccaggcgc
     1321 cgccgcacag gacccccgtc gagatccggg cggtagcgat ccgtgtccac cgcgttcggg
     1381 cgaacgacca gccgctcggg ctcgatgccg cggtccgcga gcccctgccg cagcacctcg
     1441 gagacgacga cgatcaggtc ggcgtgccgc agattggccg tctcggcctt gacgaacaga
     1501 tcgcggtgtt tgaacggccg gtcccagtga tcggcgatcc agacttccga gccgttgtat
     1561 tcgagcacga gcggaatgct ccgctcgcgc gccagcgcca cgccggccca gttggccgga
     1621 tcgaagcgct ggtacagcag gtcggccgga gcgtccgaca gccgccgccg cgccgcgttc
     1681 aggaaacgga tcgcgtgcgc gaagtacggc acttcccgga agatgttgta gcggcggctc
     1741 ggcggcacga ggtgcgccgg gtgcttcgcc ggatcgacga gggccggagc gctcgtgctg
     1801 atgaacgaga gatccgcgcc gagcgcggcg aacccggagg cgacgcccga ggtgtgcgcc
     1861 accgatcctc cggccgggac acccgaccaa aggtcggagc gcaggaagac cacccgccgg
     1921 ccgtggaccg ggcgcggccg gtgccgctcc gcctgaagca gccggtccag ctgccggcgc
     1981 gtccgccaca gcacggtccc gctgccgagc agtccgacga cgagcatcgg cagatcgcgc
     2041 acggcaaagc gtccgaccga caggacgagc cgccgtccgc gcaggtcgag cagataccgc
     2101 ttcggcgcca gcggcagcgc gccgatcgcc tgcagccgcc acagccgctc gtggaagtcg
     2161 agatcgtcgg tcaacaggac gatctcgtcc acgccgagcg ccccgagacg gcgcgccaag
     2221 ccgaccgggc cgccgccgag cgactcgcgg cgcagcgtcc gcagcgatcg tccgggcaga
     2281 aggcccggca ggagcggctc gaccttgtcc gggtcgctca cgagcagcag gaccgcggcg
     2341 gacggggagg tgagggcgct cacgcctggc gcgctccgag gagcccgcgc agatcgtccg
     2401 gggagacgac caggctcttc atcaggccga tccggccgcc gcgcaggaac gcggcgaggc
     2461 tcagcaggag catcagcccg tacgccaggc tcgaggtcaa ggccgctccg gcgattccga
     2521 agcgcgggat gaccgccacg ttcagggcga cgttgagcag cagccccgcg gccggggcga
     2581 cggccgtgat ggccggcatg ccgcgcccgc cgaagtggtt catgagcacc ccgttgaggc
     2641 cgagcgccag gacccccggc aggaggagcc gcgtcgccag gatggcgccg gagaagctct
     2701 ccccgtacag gaggcgcacc atgggaccga cgaggatccc cgcggcgagg cacaacccgg
     2761 cgagcatcat cgcggtgtga cgcagcaccc gggcggtcac ctcctcctga cgctcgctcc
     2821 gctccccggc gacacgcggc aggaggatca tgccgacggt gaccgggagc aggaggagcg
     2881 tgtcggcgat ctgcacg
//
LOCUS       DASRIQ010000443         9864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3287048, whole genome shotgun sequence.
ACCESSION   DASRIQ010000443 DASRIQ010000000
VERSION     DASRIQ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3287048"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VFD06_10825"
     CDS             complement(<1..1392)
                     /locus_tag="VFD06_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZN04062.1"
                     /translation="MPRGRSRRGEIDSVRRLGRRILDLTLDRTLGAGAGRAWSGASRG
                     AIARRFAAPPPRTGRSEAAVLRRLRAILDDSMRMSHPRLFGLFTPAPLPIAALADMAA
                     AFMNQSPDAWKAGPAATEVEAGMVRAFAREARLGPRAFGVLTGGGGAANLIALKLARD
                     RSLGIGTRRRGLHRRRAGRLRVYASDQAHFSVARALDVLGIGADALVPIPTGRDRRLP
                     VDRLARVLARDRSRGLVPMAIVATAGTTSTGNVDPLSEIARCSRRYGAFLHVDAAYGG
                     ALLFSRRHRSLLRGIERADTIAIDPHKWLFQPFSLAVLLARDRDRLRASCATEPEYLR
                     KDLEREGDRLDFYQHSLEGSRPFRGLKMDLTLQLLGRDGLGALVDRTMDVARHLERRV
                     LGDERFETCGAPVELATVVLRYLPEWARAGGTNARSRRRAPRGTPTAADRRRLDRVQL
                     RIQHEVERRGEAWF"
     gene            complement(1404..2717)
                     /locus_tag="VFD06_10830"
     CDS             complement(1404..2717)
                     /locus_tag="VFD06_10830"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZN04063.1"
                     /translation="MPAFVIVGAQWGDEGKGKIVDLLTERADIVARYGGGPNAGHTIV
                     AGGHRLALHHVPSGILNEQVTCVIGNGVVVDPASLLQEIAKLREAGVKVEENLRLSDR
                     AHVILPYHRGLDIAREEGAAAGRLGTTRLGVGPAYETKAARYGVRVADLGDPATLPEK
                     VARLAECLRRSAPAALPTECDAAPEEVSERYLQHGRALRPFVVDTSALLNKMLDDGRT
                     MLCEGAQGTMLDLDHGSYPFVTSSASTAGGACTGLGISPIRIEGVFGVLKAYATRVGE
                     GPFPTEQDNDLGQLIRKRGREFGSTTGRPRRCGWFDAVVARYAVSINQIESLALTLFD
                     VLDGFDEIPICVAYEHEGRRLEDLPADPAVLRACRPIYETVPGWREETSGITRFEDLP
                     KGAQEYVRRVEEAVGCEAGIVSVGPQREATILRPKSRIARWLPAR"
     gene            2865..4400
                     /gene="hutH"
                     /locus_tag="VFD06_10835"
     CDS             2865..4400
                     /gene="hutH"
                     /locus_tag="VFD06_10835"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZN04064.1"
                     /translation="MGTAGIVLDGRHLGLDDLRAVARGGVPVSLSAEARTRIDAAHAV
                     VRRILEGDEQVYGVNTGFGHLKDIRIPHDRLEALQLNLIRSHAAGVGAPLDAESTRAL
                     MLLRAHVLARGHSGVRPAVVASLLDHLNADLLPVVPEQGSVGASGDLAPLSHLALLLV
                     GEGEATLRGERLPAREAQYRAGLAPLRLGPKEGLSLVNGTQMIVAVGALALLEAKDLA
                     TLADLCGAASLEALLGSHHAFEARLHALRPHPGQIASAANLRTILADSPLERSHAGCG
                     RVQDAYSLRCMPQVHGAAREVIRFTEGVLATEIDAVTDNPIVFPEQGDLLSGGNFHGE
                     APALALDALAIAAAEIASISERRLERLMNPVHSGLPPFLTPDPGVNSGLMMAQVTAAA
                     LVSENKILAHPASVDSIPTEAGQEDHVSMGPIAARKARQVVRHARQVLAIEMLCACQA
                     LDLRAPLTPGRGVAAAHAAVRAAVPFMREDRFLAPDLAAVDRLLADGSLRAAVEASIG
                     TLA"
     gene            4397..6061
                     /gene="hutU"
                     /locus_tag="VFD06_10840"
     CDS             4397..6061
                     /gene="hutU"
                     /locus_tag="VFD06_10840"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZN04065.1"
                     /translation="MTRGPVRAPHGTTLSCRGWIQEAALRMLMNNLDPEVAERPDDLI
                     VYGGTGKAARNWDCFDALVRTLRTLGDDETLLVQSGKPVGVFRTHRDAPRVLLANALL
                     VPAWATWERFRELDRKGLTMFGQMTAGSWIYIGSQGIVQGTYETFAEAGRRHFGGSLA
                     GRLVVTAGLGGMGGAQPLAAAMNDAVMIAVEVDPDRIRRRLETRYVDRSAATLDEALA
                     LAREAMAKKQGLSIALLGNAADVLPEIARGPVLPDLVTDQTSAHDMLNGYVPHGLPFA
                     EALRLRKSDADEYLRRAGVSVAAHVRALLDLKRKGAVVFDYGNNIREEARQAGVANAF
                     DIPGFVPEYIRPMFCEGKGPFRWAALSGDPEDIRKTDAAVLELFPKDAALARWIRLAG
                     ERIAFQGLPARICWLGYGERARFGLRLNDMVARGELKAPIVIGRDHLDAGSVASPNRE
                     TEGMKDGSDAIADWPILNALLNTAAGASWVSVHHGGGVGIGYSLHAGMVVVADGTKEA
                     ATRLERVLTTDPGTGVMRHADAGYEEAIACARARGVRVPMLDEPGR"
     gene            6196..7377
                     /locus_tag="VFD06_10845"
     CDS             6196..7377
                     /locus_tag="VFD06_10845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HZN04066.1"
                     /translation="MVLRRGRDARARAGHLWVYAGEIERILGEPAPGSPVDVVDARGE
                     YLGTGYYNPASNIAVRLVAYAPGVVFGPDLLKARIEAALAYRERLGLTGEALRLVSSD
                     GDGIPGLTVDRYGDQLVVQIGTLGIDRLRADLTAILVDLIQPRGIFERSDLPTRTHEG
                     LGPVTGVLHGEVPDVVEVEVDGVRLEAPIRSGQKTGLFLDHRQNRREVQALADGRRVL
                     DVFCNSGGFALAALRGGAREALGIDSSAEALAAAARNAARNGVAARARFVEGNAFDLL
                     KKMERSGERFDLVVLDPPAFTKSRDSVEAARRGYKEINLRALRLASPGGIVVTASCSY
                     HIAPEAFLDILVDAAADAGREVTLLSYGGQGADHPVNLAMPETRYLKCAFLVVRAASS
                     G"
     gene            complement(7374..9233)
                     /locus_tag="VFD06_10850"
     CDS             complement(7374..9233)
                     /locus_tag="VFD06_10850"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZN04067.1"
                     /translation="MSRPMLRGVVGASLVLAVVSTTAFADDGVMLQVSHSPAPGEVRL
                     DWSGGQPAYKVYRSTSPTTIVSSANQLGETAGSLWLDAPPAGSVFYYRIVGPCLTPST
                     ENCDGVDDDCNGFVDDGCPGSCTDDGGCAANEYCDAVGQCAPDVADGQACGRDEQCVA
                     DHCSNGVCCSSGDCCTSAAQCAAHGWDLRCDAPTSCQGTSGTPICTATFQCGALTVAD
                     DSVCGGMVSQTCGPYPSIVCTGAVTQPPDQGALCADLCGDDGGCDPGAYCDATGHCIP
                     DLSLGQVCTEPSQCQSGICVDGVCCNTTCTGTCQACDLAGTSGTCTLAANGTDPDVEC
                     GAVSCTGFYHSWSGDSCRRKADVSDATATCNGAGACRSAAQECTAQSVVGPTTVTCNA
                     NCQNPNLATCNGTTAGACTNVNPGNQSCGQGVCQVTVPQCSNGAPLACVPNSGAATTE
                     TCNNIDDNCDGTVDNGSFGDGFESNNTCASVRTLPTVNSDQTLTQNTLTIYPSGDVDY
                     FRINATETDSSCACCDFFCTDEDYDLTVTLTVPAGAGSYVFCTDPVCGSVGNNCQTVN
                     AGSINSWRYDLDGECGPSTDSYSVYFRISPGNSPGFECAPYTLSYFFDAGICN"
     gene            9354..>9864
                     /gene="rmuC"
                     /locus_tag="VFD06_10855"
     CDS             9354..>9864
                     /gene="rmuC"
                     /locus_tag="VFD06_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZN04068.1"
                     /translation="MSSGLWPILTVALGLGLAVAIGLLARRRPAPPPPGPDPALTLLQ
                     QQVSDLGARLSDLGTRLVEASAAVPRDVGTAMNTLLGQMGARLAENAQAVQKIGSDTG
                     RLISDLNVRLGELGKSSQQILELGQDVRGLQQIFQAPKTRGALGEVSLNAILEQCFPA
                     EHVRIQHEFK"
BASE COUNT         1399 a         3567 c         3499 g         1399 t
ORIGIN      
        1 aaaccacgcc tcccccctcc gctcgacctc gtgctggatg cggagctgca cgcgatcgag
       61 acggcggcgg tcggccgcgg tcggagtccc acgcggcgcc cggcgtctgg aacgggcgtt
      121 cgtcccgccc gcgcgggccc attccgggag atagcgaagg accaccgtcg ccagctcgac
      181 cggcgcgccg cacgtctcga agcgctcgtc gccgagaacc cgccgctcca ggtggcgggc
      241 cacgtccatg gtgcgatcga ccagcgcccc gagcccgtcc cggcccagga gctgcagcgt
      301 caggtccatc ttgaggccgc ggaaggggcg cgagccttcg agggagtgct gatagaaatc
      361 gagccggtcc ccctcccgct ccaggtcctt tcgcaggtac tccggttcgg tcgcgcacga
      421 ggcgcgcagc cgatcccgat cccgcgccag gagcaccgcg aggctgaacg gctggaacag
      481 ccacttgtgc ggatcgatgg cgatcgtgtc ggcccgctcg atcccgcgca gaagggatcg
      541 atgacgccgc gagaagagca gcgcgccgcc ataggcggca tccacgtgca ggaaggcgcc
      601 gtatcgccgg ctgcagcggg cgatctccga cagcggatcg acgttgccgg tgctcgtcgt
      661 cccggcggtc gcgacgatcg ccatcggcac gagcccccgc gatcgatcgc gcgccaagac
      721 gcgcgccagc cgatcgaccg gcagccggcg atcccgcccg gtcggaatcg gaaccaatgc
      781 gtccgcgccg atcccgagca cgtcgagcgc gcgggcgacc gagaagtgcg cctggtcgga
      841 ggcatagacg cgcaggcgcc ccgcccgacg ccggtggaga ccgcgccgcc gcgtcccaat
      901 cccgagggac cgatcgcgcg ccagcttgag cgcgatgagg ttcgccgccc cgcccccgcc
      961 ggtcagcacg ccgaaagccc gcggaccgag ccgggcctcc cgggcgaagg cacgcaccat
     1021 cccggcttcg acttcggtcg ccgccgggcc cgctttccag gcgtcggggg actggttcat
     1081 gaaggcggcc gccatgtcgg cgagggcggc gatcggcagc ggcgccggcg tgaacaggcc
     1141 gaagaggcgc gggtgcgaca tgcgcatcga atcgtcgagg atggcgcgca accggcgcag
     1201 gaccgcggct tccgatcggc ccgtgcgagg gggcggcgcg gcgaagcgcc tggcgatggc
     1261 accgcgcgac gcgccgctcc aggcgcgccc cgccccggcg ccgagggtcc gatcgagcgt
     1321 caggtcgagg atgcgccggc cgaggcggcg gacgctgtcg atctccccgc gcctcgagcg
     1381 gccccgcggc acgcggacgc ccctcagcgc gccgggagcc agcgggcgat gcggctcttc
     1441 ggacgaagga tggtcgcctc gcgctgcggc ccgaccgaga cgatgccggc ctcgcagccg
     1501 accgcctcct cgacgcgccg cacgtattcc tgcgccccct tcgggaggtc ctcgaagcgg
     1561 gtgataccgg aggtttcctc ccgccagccg gggacggtct cgtagatcgg ccggcaggcg
     1621 cgcagcaccg ccggatcggc cggcaggtcc tcgagccgcc ggccctcgtg ctcgtaggcg
     1681 acgcagatcg gaatctcgtc gaagccgtcg agcacgtcga acagcgtcag cgccagcgat
     1741 tcgatctggt tgatcgagac ggcgtagcgc gccaccacgg cgtcgaacca gccgcagcgc
     1801 cgcggccgcc cggtggtgct gccgaactcc cggccgcgtt tccggatgag ctgtccaagg
     1861 tcgttgtcct gctcggtcgg gaacggtccc tcgccgacgc gcgtggcgta cgccttgagg
     1921 acgccgaaca ccccttcgat ccggatcggg ctgatgccca gcccggtgca ggcgccgccg
     1981 gcggtggacg ccgaggacgt gacgaagggg tacgagccgt ggtcgaggtc gagcatcgtt
     2041 ccctgcgccc cttcgcacag catcgtccgg ccgtcgtcga gcatcttgtt caggagcgcc
     2101 gaggtgtcga cgacgaacgg gcgcagcgcc cggccgtgct ggagatagcg ctcgctcacc
     2161 tcctcgggag cggcgtcgca ttcggtgggg agcgccgccg gagcggagcg ccgcaggcac
     2221 tcggcgaggc gggcgacttt ctccggcagc gtggccgggt cccccagatc ggccacccgc
     2281 acgccgtagc gggccgcctt ggtctcgtag gcgggtccga cgccgaggcg ggtcgtgccg
     2341 agtcgacccg cggccgcccc ctcctcgcgc gcgatgtcga ggccgcggtg gtacggcagg
     2401 atgacgtgcg cccgatcgct caggcgcagg ttctcctcga ccttcacccc cgcttcgcgc
     2461 agcttggcga tttcctgcag gagcgaggcg ggatcgacga ccaccccgtt gccgatgacg
     2521 caggtgacct gttcgttcag gattccggag gggacgtggt gcagcgccag gcggtggccg
     2581 ccggcgacga tggtgtggcc ggcgttcggc ccgccgccgt agcgggcgac gatgtccgcc
     2641 cgctcggtga gcaggtcgac gatcttccct tttccttcgt cgccccactg ggcgccgacg
     2701 atcacgaagg ccggcacgcg cttttctccg gcggcatcct aggaggtgac accggccgag
     2761 cgcaagggga gaaatgaccg gtcccagacg tcccggacgc cccggatccg cgtggtagac
     2821 tccggccggg tcgggcgacc cctcacacag cgaggcgcag gtccgtgggc acagccggga
     2881 tcgttctcga cggccgacat ctggggctcg acgatctccg cgcggtggcg cgcggcggcg
     2941 tgccggtgtc cctgtccgcg gaagcgcgga cccgaatcga cgcggcgcac gcggtggtcc
     3001 gccgcatcct cgagggggac gagcaggtct acggcgtcaa caccggcttc ggccacctca
     3061 aggacatccg catcccgcac gaccggttgg aagccctgca gctcaatctc atccgcagcc
     3121 acgccgccgg cgtcggcgcc cccctcgacg ccgagtcgac gcgggccctg atgctcttgc
     3181 gggcgcacgt cctcgcacgc ggccattcgg gggtgcgccc cgccgtggtc gcgtcgctcc
     3241 tcgaccacct caacgccgat ctcctcccgg tggtgccgga gcagggatcg gtcggcgcgt
     3301 cgggcgacct cgctcccctg tcgcacctcg cgctgttgct cgtcggcgag ggggaggcga
     3361 cgctgcgtgg cgagcggctc ccggcgcggg aggcgcagta ccgggcgggg ctggcgccgc
     3421 tgcggctcgg gcccaaggaa ggattgtccc tcgtcaacgg cacacagatg atcgtcgcgg
     3481 tcggcgccct cgcgctgctc gaggcgaagg acctcgcgac cctggccgat ctctgcggcg
     3541 ccgcctcgct cgaagcgctc ctcggaagcc accacgcctt cgaggcgcgc ctgcacgcgc
     3601 tgcggccgca cccggggcag atcgccagcg ccgcgaatct cagaaccatc ctcgccgaca
     3661 gtcccctcga gcgctcgcac gccggctgcg gccgcgtcca ggacgcctac tcgctccgct
     3721 gcatgcccca ggtgcacgga gccgcgcgcg aggtgatccg gttcaccgaa ggggtgctcg
     3781 cgaccgagat cgacgcggtg accgacaacc cgatcgtctt cccggagcag ggggacttgc
     3841 tctcgggagg gaacttccac ggcgaggcgc cggccctggc gctcgacgcc ctggcgatcg
     3901 ccgccgccga gatcgcctcg atctccgagc ggcggctgga gcggctgatg aacccggtcc
     3961 actccggcct gccgccgttt ctgacgccgg atcccggcgt gaactccggg ctgatgatgg
     4021 cgcaggtcac cgcggctgcg ctggtgtcgg aaaacaagat cctcgcccac cccgcgagcg
     4081 tcgactcgat tcccaccgag gccggccagg aggatcacgt ctcgatgggg ccgatcgcgg
     4141 cgcgcaaggc ccgccaggtg gtgcggcacg cccgccaggt gctggcgatc gagatgctct
     4201 gcgcctgcca ggcgctcgac ctgcgcgcgc ccctcacgcc cggacgcgga gtcgccgcgg
     4261 cgcacgctgc cgtccgcgcc gccgtgccgt tcatgcgcga agaccggttc ctcgcgccgg
     4321 acctcgccgc cgtcgatcga ctgctcgccg acggctcgct gcgcgcggcg gtcgaggcga
     4381 gcatcgggac gctggcatga cgcggggccc ggtgcgcgcg ccacacggca cgaccctctc
     4441 ctgccgcggc tggatccagg aggcggccct caggatgctg atgaacaacc tcgatccgga
     4501 ggtggcggag cgtccggacg acctgatcgt ctacggcggc accggcaagg cggcgcgcaa
     4561 ctgggactgc ttcgacgccc tggtgcgcac gctccggacg ctcggcgacg acgagacgct
     4621 cctggtgcag tcggggaagc cggtcggcgt cttccgcacc caccgcgacg cgccgcgggt
     4681 gctcctggcg aacgcgctgc tcgtcccggc gtgggcgacc tgggagcgct tccgcgagct
     4741 cgaccgcaag gggctgacga tgttcggcca gatgaccgcc ggctcctgga tctacatcgg
     4801 cagccagggg atcgtgcagg ggacctacga gaccttcgcc gaggccggtc gccggcattt
     4861 cggcggttcg ctcgcgggcc gcctcgtcgt caccgccggg ctcggcggga tgggcggggc
     4921 gcagccgctc gccgccgcga tgaacgacgc ggtgatgatc gccgtcgagg tcgatcccga
     4981 tcggatccgc cgccgcctcg agacccgcta cgtcgatcgc agcgccgcga cgctggacga
     5041 agcgctggcg ctggcgcgcg aggccatggc gaagaagcag gggctgtcga tcgcgctcct
     5101 cggcaacgcg gccgacgtcc tgccggagat cgcccgcggg ccggtccttc ccgacctcgt
     5161 caccgatcag acgagcgccc acgacatgct gaacggctac gtgccgcacg ggctgccgtt
     5221 cgcggaggcg ctgcgcctcc ggaagagcga cgcggacgag tacctgcgcc gcgccggcgt
     5281 ctcggtggcg gcgcacgtgc gggcgctcct cgacctgaag cgcaaggggg cggtcgtctt
     5341 cgactacggc aacaacatcc gcgaggaggc gcgccaggcc ggcgtcgcga acgccttcga
     5401 catccccggc ttcgtcccgg agtacatccg cccgatgttc tgcgagggga aggggccgtt
     5461 ccgctgggcg gcgctgtcgg gcgacccgga ggacattcgg aagaccgacg ccgccgtgct
     5521 cgagttgttc ccgaaggacg cggcgctcgc gcgctggatc cgcctggcgg gcgagcggat
     5581 cgccttccag ggactgccgg cgcgcatctg ctggctcggg tacggcgagc gggcccgctt
     5641 cggcctgcgt ctcaatgaca tggtggcgcg tggcgagttg aaggcgccga tcgtcatcgg
     5701 ccgcgatcac ctcgacgccg gatcggtggc ctcgccgaac cgcgagaccg aggggatgaa
     5761 ggatggatcc gacgccatcg ccgactggcc gatcctcaac gccctgctca acaccgccgc
     5821 cggggcctca tgggtctcgg tgcaccacgg aggcggcgtc ggcatcggct actcgctgca
     5881 cgccgggatg gtggtggtcg ccgacggaac caaagaagcc gccacccgcc tcgagcgggt
     5941 cctgacgacc gaccccggca ccggcgtcat gcgtcacgcc gatgccggct acgaggaggc
     6001 gatcgcatgc gcccgggccc gcggcgtccg cgtcccgatg ctcgacgagc cgggccgctg
     6061 acgcgcggaa cggcgccgcc gcggcgtccg gccgaccgcg cccggactcc ggcagcgatc
     6121 cccgtttccc ccgccgccgc aacccgtcct gccaccggcg ccggtccctc cgccggtgcg
     6181 acggccaccg gccgcgtggt gctgcggcgg gggcgcgacg cccgagcccg cgccggtcat
     6241 ctctgggtct atgcgggtga gatcgagcgc atcctcggcg aacccgcccc cggatccccg
     6301 gtcgacgtgg tggacgcgcg cggcgagtac ctcgggaccg gctactacaa ccccgcttcg
     6361 aacattgccg tccggctggt cgcatacgcg cccggggtcg tcttcggtcc cgatctcctg
     6421 aaggcccgca ttgaagcggc actcgcctac cgcgagcgtc tcgggctgac cggcgaagcc
     6481 ctgcgtctcg tctcgagcga cggcgacggg attcccgggc tgacggtgga ccgctacggc
     6541 gatcagctcg tcgtccagat cggtacgctc ggtatcgacc gcctgcgcgc cgacctgacg
     6601 gcgatcctcg tggatctcat ccagccgcgc gggatcttcg agcgcagcga cctgccgacg
     6661 cgcacgcacg aagggctcgg cccggtgacc ggcgtgctcc acggagaggt tccggatgtc
     6721 gtcgaggtcg aagtggacgg cgtccgtctc gaggcgccga tccgatcggg acagaagacc
     6781 ggcctgtttc tcgatcaccg ccagaaccgc cgcgaggtgc aggcgctcgc cgacggccgc
     6841 cgggtgctcg acgtgttctg caactccggc gggttcgcgc tggcggcgct ccggggtggg
     6901 gcgcgcgagg ctctgggcat cgacagcagc gccgaggcac tcgctgccgc cgcccgcaac
     6961 gccgcgcgca acggcgtcgc ggcgcgcgcc cgcttcgtcg aagggaacgc cttcgatctc
     7021 ctcaagaaaa tggagcggag cggcgagcgc ttcgacctcg tcgtcctcga tccacccgcc
     7081 ttcaccaaga gccgcgattc cgtcgaggcg gcgcgccgcg gctacaagga gatcaatctg
     7141 cgggccctgc gcctcgcctc acccgggggg atcgtcgtca ccgcctcctg ctcctaccac
     7201 atcgccccgg aggcgttcct cgacatcctc gtggacgccg cggcggacgc cgggcgcgaa
     7261 gtcacgctgc tgtcctacgg cggtcagggg gctgatcacc cggtgaacct ggcgatgccg
     7321 gagacccggt acttgaagtg cgccttcctg gtggtgcggg ccgcctcgtc cggctagttg
     7381 cagatgcccg cgtcgaagaa atagctgagc gtgtacggcg cgcactcgaa tcccgggctg
     7441 ttcccggggg agatcctgaa gtagacgctg taggaatcgg tcgagggccc gcactcgccg
     7501 tccaggtcat acctccagga gttgatggaa ccggcgttca cggtctggca gttgttgccg
     7561 accgagccac agaccggatc ggtgcagaac acgtacgacc cggcgccggc aggaacggtc
     7621 aaggtcactg tcagatcgta gtcctcgtcg gtgcagaaga aatcgcagca ggcacacgac
     7681 gagtcggtct cggtcgcgtt gatccggaag tagtccacgt cgcccgaggg ataaatcgtc
     7741 agcgtgttct gggttagcgt ctggtcgctg ttgacagtcg gcagggtgcg cacgctcgcg
     7801 cacgtgttgt tcgactcgaa accgtcccca aacgacccgt tgtccaccgt tccgtcgcag
     7861 ttgtcgtcga tgttgttgca cgtctcggtc gtcgccgctc cggagttcgg cacacaggcc
     7921 agcggcgcgc cgttggagca ttgcgggacc gtcacctgac agaccccctg gccgcaactc
     7981 tggtttccgg gattgacatt ggtgcaggcg ccggcggtcg tcccgttgca ggtcgcgagg
     8041 ttcgggttct ggcagttggc gttgcaggtg acggtcgtgg gcccgacgac gctctgcgcc
     8101 gtgcactcct gcgccgcgct gcggcacgcg cccgcgccgt tgcaggtggc cgtggcatcg
     8161 gagacgtccg ccttgcgccg gcagctgtcg ccgctccagc tatggtaaaa gccggtgcag
     8221 ctgaccgcgc cgcactccac gtccggatcg gttccgttcg cagccaaggt gcacgtgccg
     8281 gaagtgccgg cgagatcgca cgcttggcag gtcccggtgc aggtcgtgtt acagcagacc
     8341 ccgtcgacgc agatgccgct ctggcactgg gacggttcgg tgcacacctg cccgaggctg
     8401 agatccggaa tgcagtgacc ggtggcgtcg cagtacgcgc cggggtcgca gccgccgtcg
     8461 tcgccgcaaa ggtcggcgca cagggccccc tggtcgggcg gctgggtgac cgcccccgtg
     8521 cagacgatcg acgggtatgg accgcaggtc tggctcacca tcccgccgca caccgaatcg
     8581 tccgcgaccg tcaacgcgcc gcactggaag gtggcggtgc agatgggcgt cccgcttgtc
     8641 ccctgacagg acgtcggggc gtcgcaccgc aggtcccagc cgtgcgcggc gcactgggcg
     8701 gccgaggtgc agcagtcgcc cgaagagcag cagacgccgt tcgagcaatg gtccgcgacg
     8761 cactgctcgt cgcgcccgca ggcctggccg tccgccacgt cgggcgcgca ctggccgacc
     8821 gcatcgcagt attcgttcgc ggcgcagccg ccgtcgtcgg tgcacgatcc gggacagcca
     8881 tcgtccacga agccgttgca atcgtcgtcc acgccgtcgc aattctcggt ggatggggtg
     8941 aggcaggggc cgacgatccg gtagtagaag acgctgccgg cgggaggggc gtcgagccac
     9001 aggctccccg ccgtctcgcc gagctggttc gcggaggaaa cgatcgtcgt ggggctcgtg
     9061 gagcggtaga ccttgtaggc cggctgtccc ccgctccagt cgagccggac ttcgccgggc
     9121 gcagggctgt gcgacacctg cagcatgaca ccgtcatcgg cgaacgccgt cgtggacacg
     9181 acggcaagca ccagggacgc ccccacgacc ccgcgcagca ttggtcggga catctacgcc
     9241 ctatcgggcc gcctgtcaat ggtaatacgc cggcagtgtg acgattcagg gacaccgtct
     9301 cgcgcgggcg cgcgttgaca gtgcccggat ccggccgata gactccgacc tctgtgagct
     9361 ccggcctctg gcccatcctg accgtcgccc tcgggctggg gctcgccgtg gcgatcggcc
     9421 tcctcgcgcg gcgccgcccc gcccccccgc ctcccggacc cgacccggcc ctgaccctgc
     9481 tgcagcagca ggtgtccgac ctcggcgcac ggctgtcgga cctggggacc cgcctcgtcg
     9541 aggcgagcgc ggcggtgccg cgcgacgtcg ggacggcgat gaacacgctg ctcggtcaga
     9601 tgggggcgcg cctcgccgag aacgcccagg cggtgcagaa gatcggcagc gacaccggtc
     9661 ggctcatttc cgacctgaac gtgcggctcg gcgagctggg aaagagcagc cagcagatcc
     9721 tcgagctggg acaggacgtc cgcggcttgc agcagatctt ccaggctccc aagacccgcg
     9781 gcgcgctcgg cgaagtgtcg ctcaacgcga tcctcgagca gtgcttcccc gccgagcacg
     9841 tgcgcatcca gcacgagttc aaga
//
LOCUS       DASRIQ010000444         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2255646, whole genome shotgun sequence.
ACCESSION   DASRIQ010000444 DASRIQ010000000
VERSION     DASRIQ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255646"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFD06_10860"
     CDS             complement(<1..354)
                     /locus_tag="VFD06_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN04069.1"
                     /translation="MNDLTIREASRLIGRTPATLRRYIRSGRLAAAKEDGKFGEEYRI
                     RREDLLALGFTPTAPDEELPQEALVRASSAPPAKVAQDAVPISLYNELLMKHEQILVQ
                     YGMIRAGGQKLLEFKA"
     gene            complement(503..1198)
                     /locus_tag="VFD06_10865"
     CDS             complement(503..1198)
                     /locus_tag="VFD06_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04070.1"
                     /translation="MTSTPALLVLSACSTAIIHALIPDHWLPFALLARAENWSERRLL
                     SLVGLTGALHVLVSLSLGAALALAGSAAARMAERIGTSLETIAGALLILFGIAYGVWA
                     HRREARAHDRGVAAHDGPTPARLHAHGHLLSRWVGHRASAWALVAIIGISPCVLLQPI
                     LFASAAEGTGVALAAGAGFAACTIATMLVVTAVARRGLERIALVFFTHWGDLLSGLII
                     AAIGVVVVCEGLG"
     gene            1365..2060
                     /locus_tag="VFD06_10870"
     CDS             1365..2060
                     /locus_tag="VFD06_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04071.1"
                     /translation="MPKHPLASCLAAALVCAALPRIAAAGEAGRRPTPGAEATGAPAW
                     RETGVSLPVRFDERLKREALRYTDLAAPRALSPLADQDFEDDEESRAARSRRVERLLT
                     RAIRRALDDHLSDLARTSPAFEGLFRVLDAREADTARREPAAMPHGGATSAAPARGFE
                     ADVRVKIDAHPRLQMRARMGAVTGSIEIPVLDPEVRVGLERPLGTRGRAALQGGWSDG
                     RGEWAAVALSFSF"
     gene            complement(2073..>2308)
                     /locus_tag="VFD06_10875"
     CDS             complement(2073..>2308)
                     /locus_tag="VFD06_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZN04072.1"
                     /translation="RGPYSGAIGYLSRDGGCDFNIVIRTLACGGGRAHFRVGGGIVAD
                     SDPEREWLETLDKARALLAVLGVRLDETGRVGG"
BASE COUNT          316 a          869 c          835 g          288 t
ORIGIN      
        1 ggccttgaac tccaggagct tctgccctcc ggcccggatc atcccgtact ggacgaggat
       61 ctgctcgtgt ttcatgagca gctcgttgta gagggagatc ggaacggcgt cctgcgcgac
      121 cttcgccggc ggcgcggagg aggcgcgcac gagcgcctcc tgcggcagct cctcgtccgg
      181 cgccgtgggg gtgaatccga gcgccagcag gtcctcgcgc cggatgcggt actcctcgcc
      241 gaacttccca tcctccttgg cggccgcgag ccggccggag cggatgtagc ggcgcagggt
      301 ggcgggagtg cggccgatga gccgcgacgc ctcccggatc gtcaggtcgt tcatccttct
      361 cctccgggac tcctctcctc cgggatctcc tccgagggca ctcggccgcg gcgcgtcgag
      421 gcgcgggcgt tataccagaa cgccgcggcg caagtaaaga acggcgcggc gccgaagcgc
      481 cgcgccccac ggcgatcgcc cttcatccga gcccctcgca caccaccacg acgccgatgg
      541 cggcgatgat gaggccgctg agcagatccc cccagtgcgt gaagaagacg agggcgatgc
      601 gctccagccc ccggcgcgcc accgcggtca cgacgagcat cgtggcgatc gtgcacgccg
      661 cgaatcccgc gccggcggcg agcgccacgc cggtcccttc ggccgccgac gcgaacagga
      721 tcggctgcag cagcacgcac gggctgatgc cgatgatcgc cacgagcgcc cacgccgaag
      781 cccggtgtcc gacccagcgg gacagcaggt ggccgtgcgc gtgcagccgg gcgggcgtcg
      841 gtccgtcgtg cgccgcaacg ccgcgatcgt gcgcccgcgc ctcgcgccgg tgcgcccaca
      901 ccccgtaggc gatcccgaag aggatcagga gggcgccggc gatcgtctcg agcgacgtcc
      961 cgatgcgttc cgccatccgc gccgcggcgc tcccggcgag cgccagcgcc gccccgagcg
     1021 acagcgacac gaggacgtgc agcgcgccgg tcagcccgac gagcgacagg agccggcgct
     1081 cgctccagtt ctcggcgcgc gccaggagcg cgaacggcag ccagtgatcg ggaatgaggg
     1141 cgtggatgat cgcggtgctg caggcggaca ggacgagaag ggccggtgtg ctcgtcacgg
     1201 ggctccacgc cggggcgcgg cgcggcggcg gatgttagca cagacgtttt cgtctccggc
     1261 cgcgtcagaa acccgacgcg gcggcggcgc gccggcgcgg ttcacgatcc gctgaatgcc
     1321 ttgtcctgat tgcgttccag aggaatcgac cgtatatgga ggcgatgccc aagcacccgc
     1381 tcgcctcgtg cctcgccgcg gcgctggtgt gcgcggcgct cccccggatc gcggcggccg
     1441 gcgaggcggg ccgccggccc acgcccggag ccgaggcgac cggcgccccg gcctggcggg
     1501 agaccggcgt ctccctgccg gtccggttcg acgagcggct gaagcgcgag gcgctgcgct
     1561 acaccgacct cgccgccccg cgtgccctct cgccgctggc cgaccaggac ttcgaggacg
     1621 acgaggagag ccgcgccgcg cgctcccgcc gcgtcgagcg cctgctgacg cgcgccatcc
     1681 gccgcgcgct cgacgatcac ctcagcgacc tggcgcgcac ctcccccgcc ttcgagggac
     1741 tgttccgcgt cctcgacgcc cgcgaggcgg acaccgcgcg gcgcgaaccc gccgcgatgc
     1801 cccacggcgg tgcgacgtcc gccgccccgg cccggggctt cgaggccgac gtgcgggtga
     1861 agatcgacgc ccacccgcgc ctgcagatgc gggcgcggat gggagcggtc accggttcga
     1921 tcgagatccc ggtgctcgac ccggaagtgc gcgtgggcct cgagcggccg ctcgggacgc
     1981 gcggacgcgc cgccctgcag gggggatggt cggacgggcg cggcgagtgg gcggcggtcg
     2041 ctttgagctt ctccttctag ccgccgatcc ttctagccgc cgactcgacc cgtctcgtcg
     2101 agacggacgc cgagcacggc gagcagcgcg cgcgccttgt cgagcgtctc gagccactcc
     2161 cgctccggat cggaatcggc cacgatcccg ccgccgacgc ggaagtgcgc ccgcccgccg
     2221 ccgcacgcga gcgtccggat cacgatgttg aagtcgcagc cgccgtcgcg cgacaggtag
     2281 ccgatcgccc ccgagtaggg gccgcgct
//
LOCUS       DASRIQ010000445         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2256152, whole genome shotgun sequence.
ACCESSION   DASRIQ010000445 DASRIQ010000000
VERSION     DASRIQ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256152"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFD06_10880"
     CDS             complement(<1..599)
                     /locus_tag="VFD06_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04073.1"
                     /translation="MEKNDGVFDDFKPRDLPRSIRTDARSIYRFYLDEERRADLEKMR
                     TPKRVLTLPLWLFRSLLLKLTPQRRTLLIVALILAFLGGIHFRFQDVDLSFDATPWAF
                     LMVLFVLALELKDKLLAKDEIAVARQVQLAILPSVDPAWPGYALWSHTRPANDVGGDL
                     IDFIEEPSPSSLGIALGDVAGKGLGAALLMAKLQASLRA"
     gene            complement(648..1229)
                     /locus_tag="VFD06_10885"
     CDS             complement(648..1229)
                     /locus_tag="VFD06_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN04074.1"
                     /translation="MRPEEEIFAAVERGYTARVEALLDEAPEAVHARNERGDSVLLTA
                     AYYGRKDLFDRLVARGADVSLHEAAALGFVDHARAHLDASPGSVGVHSHDGFTPLHLA
                     AFFGHRDLAQLLLDRGADVNARSKSERFARSNTPLHAAAANKQVEVARLLVERGADVN
                     AKDGHGFTPLALAANSKSDLLMLLLLEQGARAE"
     gene            complement(1326..2462)
                     /locus_tag="VFD06_10890"
     CDS             complement(1326..2462)
                     /locus_tag="VFD06_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZN04075.1"
                     /translation="MRSAPPHRAAVPRVIVPGFAFVIAVTVLCGCGGGMSARVAAEPA
                     VRIDRPAPATPQATARSGFEEESVSEERLDDAGATAQPTQPAPPASRDRRDAANAPRN
                     RSDQPETSRARREASHKNEDAAADDAPPLRHVLRRGQTLYSVAKMYGVEVAALMQANG
                     IKNARHVAANQMLVIPVPRTAKSASPGPKEGGVMQASLAPGLGLPDAEDSPSPKKKKD
                     ARGDDARAAAQPALAWPVQGAVTASFGRRGKSDHHEGIDIDGDTGDPIRAAASGIVAF
                     AGNHGDYGRTVVIDHGEGWRTIYAHASTLKVEEGDPVRVGDTIAEVGRSGNARGTHLH
                     FEVRREGRAVNPIPYLRSSDTLTAGVVPAPKPRSKQAKGKSAPR"
     gene            2551..>3265
                     /locus_tag="VFD06_10895"
     CDS             2551..>3265
                     /locus_tag="VFD06_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04076.1"
                     /translation="MRATRSILLLLGLLPAALLGQAGIAPAHAQSIVSSEVDPVQSRT
                     GDRVVFRATIEMPQDCAWQADAEIQFGTQPELGPGTGWGLDLTLSPGTITCLPGAIQF
                     TRENDLGLLPVAAGPGVARLVVDGTVADTAPFNLVVNPGPAPGWSDPSADAQLQILIQ
                     TAGIAAYGTLLAVADNLHHAIDFLDPLPGIVTGGFRSPGNGNVRGLATDGDNFFVSVA
                     DSFGPRLYRVNANGVVQDAW"
BASE COUNT          441 a         1131 c         1196 g          497 t
ORIGIN      
        1 atggcgcgca ggctcgcctg cagcttcgcc atcagcaggg ccgccccgag ccccttgccg
       61 gcgacgtctc cgagggcgat gccgagcgag gatggcgatg gctcctcgat gaagtcgatc
      121 agatcgccgc cgacgtcgtt cgccggccgc gtgtggctcc agagggcgta gccgggccag
      181 gccggatcga ccgacggaag gatcgccagc tgcacctggc gggccaccgc gatctcgtcc
      241 ttcgccagga gcttgtcctt gagctcgagc gccaggacga acagcaccat caggaacgcc
      301 cacggggtgg cgtcgaacga caggtccacg tcctggaagc ggaagtggat gccgccgaga
      361 aacgccagga tcagggcgac gatgaggagc gtccggcgct gcggggtcag cttgaggagc
      421 aggctccgga agagccacaa cggcagtgtg aggacccgct tcggggtccg catcttctcg
      481 aggtcggcgc gccgctcctc gtcgaggtag aagcggtaga tcgaccgggc gtcggtccgg
      541 atcgagcggg ggagatcgcg cggcttgaag tcgtcgaaga cgccgtcgtt cttttccatg
      601 tgaggccgcg ccggctactt cttcttcgcg aagggacggc ggcgtgacta ttcggcccgc
      661 gcgccctgtt cgaggaggag gagcatcagg aggtcgctct tgctgttggc ggcgagcgcc
      721 aggggcgtga agccgtggcc gtccttcgcg ttcacgtcgg cgccgcgctc gacgaggagc
      781 cgcgccacct ccacctgctt gttggccgcc gcggcgtgga gcggcgtgtt ggaccgcgcg
      841 aagcgctcgc ttttcgagcg cgcgttgacg tcggcgccgc gatcgagaag caactgcgcc
      901 aggtcgcggt gcccgaagaa ggcggcgagg tgcagcggcg tgaagccgtc gtggctgtgg
      961 acgccgacgc ttccggggct cgcgtcgagg tgcgcgcggg cgtggtcgac gaagccgagc
     1021 gccgccgcct cgtgcaggct gacgtcggcc ccgcgtgcga ccaggcggtc gaacaggtcc
     1081 ttgcggccgt agtaggccgc cgtcaggagc accgaatcgc cgcgctcgtt gcgggcgtgc
     1141 accgcctccg gcgcctcgtc gagcaacgcc tcgacgcgcg ccgtgtagcc gcgctcgacc
     1201 gccgcgaaga tctcttcttc cggtcgcatg ggagtgccta tcctagaaca ggagcgccgg
     1261 aactcaaaga ggcagcggcc ggcggccggc cgccgcggcg gatccgacgc ggggcgctgt
     1321 ggcggtcagc gcggcgccga ttttcccttc gcctgcttgg agcggggctt cggagccgga
     1381 acgactcccg cggtcagcgt gtcgctggat cggagatacg ggatcgggtt caccgcacgc
     1441 ccctcgcgcc gcacctcgaa gtgcaggtgg gtgccgcggg cgttgccgga gcgaccgacc
     1501 tcggcgatcg tgtcgccgac ccgcaccgga tccccctcct ccaccttgag ggtgctggcg
     1561 tgcgcgtaga tggtgcgcca gccctcgccg tgatcgatca ccacggtccg gccgtagtcg
     1621 ccgtgattcc ccgcgaacgc cacgatcccg gaggcggcgg cccggatcgg gtcgccggtg
     1681 tccccgtcga tgtcgatccc ttcgtggtgg tcggacttgc cgcggcggcc gaacgacgcg
     1741 gtgacggcgc cctgcaccgg ccaggcgagc gccggctggg ccgcggcgcg cgcgtcgtcg
     1801 ccgcgggcgt ccttcttttt cttcggcgag ggggagtcct cggcgtccgg caggccgagc
     1861 cccggtgcga gcgacgcctg catcacgccc ccctccttgg gaccggggga ggcgctcttc
     1921 gcggttcgag ggaccgggat gaccagcatc tggtttgcag cgacgtgacg cgcgttcttg
     1981 atgccgttgg cctgcatcag agccgccacc tcgacaccgt acatcttcgc gacggaatag
     2041 agggtctgac cgcggcgcag cacgtgccgc agcggggggg cgtcatccgc ggccgcatct
     2101 tcgtttttgt gcgaggcctc gcgccgcgcg cgcgacgtct ccggctggtc gctccggtta
     2161 cgcggcgcat tggcggcgtc ccgccggtcg cgcgatgccg gcggcgccgg ttgcgtgggc
     2221 tgcgccgtgg cgccggcgtc gtcgaggcgc tcctcgctca ccgactcctc ctcgaatccg
     2281 gaacgggcgg tggcctgagg cgtcgccggc gcggggcgat cgatgcggac cgccggctcg
     2341 gcggcgacgc gcgcgctcat ccccccgccg cagccgcaca ggacggtgac ggcgatcacg
     2401 aaagcgaaac cgggaacgat gacgcgcggg accgcggccc ggtggggcgg agcgcttcgc
     2461 atcgacacta atatggccga cgccggccgg ttcgtccaga gcctccgtca tccagttgac
     2521 ggagggccga actcggcgta agcagggggg gtgcgggcca cccgctccat cctcttatta
     2581 ctcgggctac tcccggcggc ccttctgggc caggccggga tcgctcccgc gcacgcccag
     2641 agcatcgtct cctcggaggt tgatccggtt cagagccgga ccggcgatcg ggtggtgttc
     2701 cgcgcgacga tcgagatgcc gcaggactgc gcctggcagg ccgatgccga gatccagttc
     2761 ggcacgcagc cggagctcgg tccgggaacc gggtgggggc tcgacctgac gctcagcccg
     2821 gggacgatca cctgcctgcc gggggcgatc cagttcacgc gcgagaacga cctggggctg
     2881 ctgccggtcg ccgccggtcc gggcgtggcg cggctcgtcg tcgacgggac ggtcgccgac
     2941 accgcgccgt tcaacctggt cgtcaacccg ggaccggccc ccggctggag cgatccctcc
     3001 gccgacgcac agcttcaaat cctgattcag acggccggga tcgcggcgta cggcacgctg
     3061 ctcgccgtcg ccgacaatct gcaccacgcg atcgatttcc tcgatccgct gccggggatc
     3121 gtgaccggag gcttccgctc ccccggcaat ggcaacgtgc ggggcctggc gaccgacggc
     3181 gacaacttct tcgtgtcggt cgccgacagc ttcggcccgc gcctctaccg cgtcaacgcc
     3241 aacggggtcg tgcaggacgc ctggg
//
LOCUS       DASRIQ010000446         9719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3574877, whole genome shotgun sequence.
ACCESSION   DASRIQ010000446 DASRIQ010000000
VERSION     DASRIQ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3574877"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1240)
                     /gene="sat"
                     /locus_tag="VFD06_10900"
     CDS             complement(95..1240)
                     /gene="sat"
                     /locus_tag="VFD06_10900"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HZN04077.1"
                     /translation="MTTQPHGGVLVDRTLTKEARAEALRRAERLPALDLGERQAREVE
                     NIALGVYSPLAGFLTSADFDAVLKTSRLASGVPWTIPIVLDVNDADAARLGRGEILLR
                     HEGRALATMPVEEAYPLRRDDYAQAVFGTLDPKHPGVAHAATMGTMLLGGPVTLIDLL
                     PTPFPAHRLTPRETRARFEANGWKTVVGFQTRNIPHVGHEALQKAALNLTDGLFINPL
                     VGGKKAGDFEDPVIVAAYDALVKHYYPADRAVLGILQGQMRYAGPKEAIFHAIQRKNF
                     GCSHFIIGRDHAGVGNFYHPYAAQEIFKEFPDLGVTPLFFPAFFKCRKCQGMVNEKIC
                     PHRGDDRVDFSGTRLREAILGGDDFDGMIRPEVSAAIRAVDGGPIRT"
     gene            1649..2905
                     /locus_tag="VFD06_10905"
     CDS             1649..2905
                     /locus_tag="VFD06_10905"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN04078.1"
                     /translation="MSQSLTCVALLLTAAWTAFGVLFLRGARQLRHLDEELDRESQGP
                     VPATGTGAVPSLAVVVTARNEAGAIEGTVRSLMRQRHPALHVVVVDDRSTDATPSVLS
                     RLAAEADARLEVVRIEVLPDGWLGKCHACHAGARRARGDWILFMDGDVTLAREDLLER
                     TVALAARRGIDHLPILPDMRPTGLMQGALLANFEQGLLLHGRFWEMDRDRPRGGSGVG
                     AFNLVRRAAYDRVGGHTLLKMEVAEDYKLGMLLKESGARQRLWSGLGVVACPWLRGVA
                     EMMRGLEKNFFGGVGYSLWTVAWQTSLAAILQIGPLLLALFGGSLWLALPWLVQQGFL
                     VAILRSESDRLGHPAPALWLAYPLAVPLILFAYWNSALKTLAQGGIHWRDTFYPLAAL
                     KAGAVRPGDWRRRGGRGPVALSEDRL"
     gene            2913..4097
                     /gene="ispG"
                     /locus_tag="VFD06_10910"
     CDS             2913..4097
                     /gene="ispG"
                     /locus_tag="VFD06_10910"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HZN04079.1"
                     /translation="MSQDSPASPPPRRPTRPVRIGRITIGAGHPIAVQSMTATKTTDV
                     EATVRQVLDLERAGADVVRIAVDGAKDAAALAEIRARTRANLSVDLQENYRLAQDVAP
                     HVDKVRYNPGHLHHHERNKPVRDKVAFLAEVAERHDVALRVGVNCGSVDPDKAGRFGG
                     DSVAEMVESALEHAALLDDLGFTRFCVSLKDSDPAKVIDANRRFSERRPDVPLHLGVT
                     EAGLPPDGVIKTRIAFEQLLAAGIGDTLRVSLTVPNDRKGEEIAAGRQILDDIAHNRF
                     RSVPPNFGRGLNIISCPSCSRVENEAFVELAQQVKEMTAYAREHALTIAVMGCRVNGP
                     GETDDADLGLWCAPTFVNLKRGSEDLGSFRYDEVLSRLKCELDRLIAARRDRNAAATR
                     RP"
     gene            4205..5215
                     /locus_tag="VFD06_10915"
     CDS             4205..5215
                     /locus_tag="VFD06_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HZN04080.1"
                     /translation="MAARFAGLALACVGKEYPNKIAHVLQSDADALPPRRLTPAFFGC
                     YDWHSSVHGHWLLARLARSFPSASFSAPARAALAKNLTTTNIKAEAAYLSGKGRESFE
                     RPYGLAWLLTLAAELREWDDPQARSFSAALEPLETAAAKKLSTWLPKLTHPIRVGEHD
                     QTAFALGLVLDWARGKKDPVMTALAIERARSYYLRDRGCPLTYEPSGQDFLSPCLAEA
                     DLMRRILAPGPFADWLTTFLPDLPRDGSAAWLQPGVVTDPTDGKLAHLDGLNLSRAWM
                     LEGIASGLPTADPRVAALRAAADRHRKDGLAAVTGAHYEGGHWLGTFATYLTTGRGLQ
                     DR"
     gene            complement(5231..>9719)
                     /locus_tag="VFD06_10920"
     CDS             complement(5231..>9719)
                     /locus_tag="VFD06_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZN04081.1"
                     /translation="IATLLDYDAVLLVVNEPCLDRFALGDVLADYADAGGGVVLTVAS
                     FFDRWAVPGRFERDGYHPLVQESAARRASSLGAFDAAHPIMAGVTAATGMLIPDMGPA
                     PGASILAAWASGEPLVAVKAANVVAVNAFVQAGRWTGDMPLLLRNAIVWAARTRAAWL
                     TVEPSGGVVPAGEQADIALRFDAAGLPAANHDATLVIRTNDPNLEEFPLPVRLRAAGA
                     PDIEVSGQEVALESSRDFVTEKAWTAHSIAAPVPPEGGGFIDLVAIGDHDGDDGLEQA
                     LFRVESAFTEEVNHAGAACATAEMRVPMDEAELASLLADGRLDVQVQNGLYSEAVCPV
                     NRHTIRLRYTGPGAAIDFGKVFAGASRALRFAIANAGSDPLEVSDLASDHPAFAPGTG
                     ALALLPGERRAIDVTFTPGGAGTYEATLTITSNDPDEPVRTLALRGHGMAAPEIEVIP
                     GSLDVSAAPGRIVTWPLTIDNRGASPLAFEVDRDIHRGTAAAAEGSVLLVQDVQPDGR
                     NANEWLLSSQRLSYDTIRSTQIAATDLPAYRLVILAGGQPSSYYAALAARQAQIDAYV
                     ASGGCLEYHAWSPVWAGEPSPFVLPGGGTTHSLQATFNTLLEPHHPLAAGIQAPAFLA
                     GSRAYVTGLPPTAVRIAEAGPGRTTLAVSGLGRGSVIVATTILETFFTTREAAWFLLE
                     TMIPYAHALPVAPVSIVPASGEAPAGSARTLTVALDTSGLPAGERDYHLLVRSNDPDE
                     PTVRVPFHVDVGRAPALRVSATALDFGTVFLDAPVARTLRVDNAGNEDLEVALEIDSP
                     LYSATPASLRVGPGEGRDVSVRVLSGAEGIQAGTLRLLSNDPGDPEVVVPLTATVHRP
                     PVLAVSPATLLASLPEGHSTTLPMVIANPGHFDLTFFVTSGASWMRALPLSGSVAPGG
                     AVEITVTFDAGTSLPGLHEATLQIQSNDPARPLLAIVAHLTVDRDNDRDGVPDVTDNC
                     PVFPNAGQQDADADGTGDACDSCVAIPNPLQDDRDGDRIGDACDNCPAVFNVPQADVD
                     ADGRGDQCDNCQDAANFGQEDANSDLAGDACQPRFVVGTLVRDGGTLRLPTRVEEPQG
                     EPVAGVVRLTGALDALIRLSDSLASDDCADGYFVDGVPGAGFGFANGAVGSPFLFDLD
                     AIFACDDGAPDYLLAPGECAGNGIPFEPILSLEGLTLPATICVRRAVGGGTGFELTVI
                     RFDEAAIDLRLLDPQAVVVDVPFADRLPSRIELSALIEGSPYVIDLSASDGKTPPVAA
                     RVEFLHGGEDELRFDGRPAAAVAGAGTFECESPDGALVTLDGSASSDPDASAGTPGSL
                     TFEWRQDPWTPADTLLGTGAVLSVTLPLGTHQLALRVTDAGGESDTEPFTVTVRDTMP
                     PALALQATPTTLWPPNHTLRPVHLTWTMSDRCDAGAAVALVAVTSSEADAATGASDGS
                     TAGDVGDAQIGTADADLLLRAERTGSGPGRFYDLMYMASDASGNGTLAVARIEVPRRP
                     R"
BASE COUNT         1439 a         3423 c         3453 g         1404 t
ORIGIN      
        1 aaacggcgga agacgagatc gggagcggcg cagtcgagcc cgatgacggc gaccttcaca
       61 tggactcccg acgccccggg ggggcgcccg caactcaggt tcgaatgggc ccgccgtcga
      121 ccgcgcggat ggcggccgag acctccggcc ggatcatccc gtcgaagtcg tcgccgccca
      181 ggatcgcctc gcgcagccgc gtgccgctga aatcgacccg gtcgtcgccg cggtgcgggc
      241 agatcttctc gttcaccatc ccctggcact tccggcactt gaagaaggcg gggaagaaca
      301 gcggtgtgac gccgagatcc gggaactcct tgaagatctc ctgcgccgcg tacgggtggt
      361 agaagttgcc gacgccggcg tggtcccgcc cgatgatgaa atggctgcag ccgaagttct
      421 ttcgctggat ggcgtggaag atcgcctcct tcggaccggc gtagcgcatc tgcccctgca
      481 ggatcccgag gaccgcccgg tcggccgggt agtagtgctt caccagcgcg tcgtacgccg
      541 cgacgatgac cgggtcctcg aaatcccccg ccttcttgcc gccgacgagc ggattgatga
      601 acagcccgtc ggtcaggttc aaggccgcct tctgcagcgc ctcgtggccg acgtgcggga
      661 tgttgcgggt ctggaagccg acgaccgtct tccagccgtt cgcctcgaac cgggcccgcg
      721 tttcgcgcgg cgtcagccgg tgggccggga acggcgtggg gaggagatcg atgagggtga
      781 ccgggccgcc gaggagcatc gtccccatgg tggcggcatg cgccacgccg ggatgcttcg
      841 ggtcgagcgt cccgaacacc gcctgcgcat agtcgtcacg gcgcagcggg tacgcctcct
      901 cgaccggcat cgtcgcgagc gcccggccct cgtggcgcag gagaatctcg ccgcgcccga
      961 ggcgggcggc gtcggcgtcg ttcacgtcga ggacgatcgg gatggtccag gggacgccgc
     1021 tcgcgagccg gctggtcttc aggacggcgt cgaaatcggc gctcgtgagg aaccccgcga
     1081 gcggcgagta gacgccgagg gcgatgttct cgacctcgcg cgcctgacgc tcgccgaggt
     1141 cgagcgcggg aaggcgctcg gcgcggcgca gggcttcggc gcgcgcttcc ttcgtgaggg
     1201 tccggtcgac caggactccg ccgtgcggtt gtgtcgtcat gtcgttcgtt ggcctctggg
     1261 attatgcaga aaaaagcgga ggcggccagt ggagccccat gcgggccgtc ccatcgcgcg
     1321 tcgcgcccgg ctcattcgga ggcggcgagc cctcccacgc cggcggtcag ccagaccgtg
     1381 accccctgac gacgctgtgc agaattcgac tccgcactca ttcggccccc caccccgacg
     1441 ggccccccaa aggcccggaa tgcagatcga gcgcgggcgc cggtgcggcg ctggccgcgc
     1501 acaagtctca aattcggtag gcgtcttata caacaggaag gaaccgaagc aaccggaaaa
     1561 ttgcgctacg gcgcaacatt tcacggttgt tgcgctccag cgcggcgttt ccggaggctt
     1621 tcgagcgacg gtgtatggta ggcgtccgat gagccagagc ctcacctgcg tcgcactcct
     1681 cctgaccgcc gcctggacgg cgttcggcgt cttgttcctg cgcggggccc gccagctccg
     1741 gcacctggat gaagagctgg accgcgaatc gcaaggcccg gtgccagcca ccggaactgg
     1801 cgcggtcccg tcccttgccg tggtggtcac ggcgcgcaac gaagcgggag cgatcgaggg
     1861 aacggtgcgc agcctcatga ggcagcgcca tcccgctctg cacgtcgtcg tggtggacga
     1921 tcgctccacc gacgccacgc cctcggtcct cagccggctc gcggccgaag cggacgctcg
     1981 actggaggtc gtgcgcatcg aggtcctccc ggacggctgg ctggggaagt gccacgcctg
     2041 ccacgccggc gcccggcgcg cgcgcggcga ctggatcctc ttcatggacg gcgacgtcac
     2101 cctggcccgc gaggacctgc tggagcggac ggtggcgctc gcggcgcgcc gcggcatcga
     2161 ccacctgccg atcctcccgg acatgcgccc caccggcctc atgcaggggg cgcttctggc
     2221 caacttcgag caggggctgc tcctgcacgg ccgcttctgg gagatggatc gcgaccggcc
     2281 gcgcggcggc tcgggcgtcg gggcgttcaa cctcgtgcgc cgcgccgcct acgaccgggt
     2341 cggagggcac actcttctga agatggaagt ggcggaggac tacaagctcg gaatgctcct
     2401 caaggagagc ggcgcccgcc agcggttgtg gagcgggctc ggggtcgttg cctgtccgtg
     2461 gcttcgcggg gtggcggaga tgatgcgcgg gctcgagaag aacttcttcg gaggggtggg
     2521 gtactcgctc tggacggtgg cgtggcagac gagcctcgcc gcaatcctgc agatcggtcc
     2581 gctcctcctc gcgcttttcg ggggctcgct ctggctcgcg ctgccgtggc tcgtgcagca
     2641 gggattcctg gtcgccatcc tgaggtccga atcggatcgg ctcgggcatc cggctcctgc
     2701 gctgtggctc gcctatccgc tcgcggtccc gctcatcctg ttcgcctact ggaactccgc
     2761 cctcaagacc ctggcccagg gggggatcca ctggcgcgac accttctatc ccctggcagc
     2821 cctcaaagca ggggcggtcc ggccggggga ctggcggcgg cgcggcggac gggggccggt
     2881 ggccctgtcc gaagaccggt tatgatctcc gggtgagtca ggattccccc gcaagccctc
     2941 ccccgcgccg gccgacccgg ccggtgcgca tcggcaggat caccatcggc gccggccatc
     3001 cgatcgcagt gcagagcatg accgcgacga agacgaccga cgtcgaagca accgtacgcc
     3061 aggtgctcga cctcgagcgg gccggcgccg acgtcgtccg gatcgccgtg gacggggcga
     3121 aggacgccgc cgccctcgcg gagatccgcg cccgcactcg cgccaacctg tcggtggacc
     3181 tgcaggagaa ctaccgtctc gctcaagatg tggccccgca cgtggacaag gtgcgctaca
     3241 acccggggca cctgcaccat cacgagcgga acaagccggt gcgcgacaag gtggcgttcc
     3301 tggccgaggt ggcggagcgc cacgacgtcg ccctgcgcgt cggcgtgaac tgcggctcgg
     3361 tcgatccgga caaggcggga cgcttcggcg gcgattcggt ggcggagatg gtcgagtcgg
     3421 ctctcgagca cgccgccctg ctcgacgatc tcggcttcac ccgcttctgc gtgtcgctca
     3481 aggactccga cccggcgaag gtgatcgacg ccaaccggcg cttctcggag cgccgccccg
     3541 acgtgccgct gcatctcggt gtcaccgagg ccggcctgcc gccggacggc gtcatcaaga
     3601 cccgcatcgc cttcgagcag ctcctcgccg ccggcatcgg cgacacgctg cgggtctcgc
     3661 tgaccgtgcc gaacgaccgc aagggggagg agatcgccgc cggccggcag atcctcgacg
     3721 acatcgcgca caaccgcttc cgctcggtgc cgcccaactt cgggcgcggc ctcaacatca
     3781 tctcctgccc ctcctgctcg cgggtcgaga acgaggcgtt cgtcgagctg gcgcagcaag
     3841 tgaaggagat gaccgcctac gcccgcgagc acgccctgac catcgcggtg atggggtgcc
     3901 gggtcaacgg ccccggagag accgacgacg ccgatctcgg cctgtggtgc gccccgacct
     3961 tcgtgaatct caagcgcggt tcggaggatc tcggatcgtt ccgctacgac gaggtgctct
     4021 cccgcctcaa gtgcgagctc gatcgcctga tcgccgcccg gcgggatcgc aacgccgccg
     4081 cgacccgccg cccgtgagcg gccgcgcagc ggtcgccgcg cttctccttg cgctcgcgct
     4141 cccttccgct tctccagctt ccgcggatct catcgatccg ctcccgctgc tcacggatga
     4201 agccgtggcg gcccgcttcg cgggcctggc gctcgcgtgc gtgggcaagg agtacccgaa
     4261 caagatcgcg cacgtgctgc aatccgacgc cgacgcgctg ccgccgcgcc ggctgacccc
     4321 ggcgtttttc ggctgctacg actggcactc gtcggtgcac gggcactggc tgctcgcccg
     4381 cctggcgcgc tctttcccct ccgcctcgtt ctccgcgccg gcgcgggccg cgctggcgaa
     4441 gaacctcacg acgacgaaca tcaaggcgga ggccgcatat ctgtccggga aggggcggga
     4501 gtcgttcgag cgcccgtacg gtctcgcctg gctcctgacc ctcgcggccg agctgcgcga
     4561 atgggacgac ccgcaggcgc ggtccttttc ggcggcgctc gagccgctcg agacggcggc
     4621 ggcgaagaag ctctcgacct ggcttccgaa gctgacgcac ccgatccggg tcggcgaaca
     4681 cgaccagacc gccttcgcgc tcggcctcgt cctcgactgg gcccggggaa agaaagaccc
     4741 ggtgatgacg gcgctcgcca tcgagcgggc ccggtcgtat tacctgcgcg accgcggctg
     4801 cccgctcacc tacgagcctt cggggcagga cttcctttcc ccctgcctgg cggaggcgga
     4861 cctgatgcgg cgcatcctcg cccccggacc gttcgccgac tggctgacga cgttccttcc
     4921 cgatctgccg cgcgacggct cggctgcgtg gctccagccg ggggtcgtga ccgacccgac
     4981 cgacggcaag ctcgcccacc tcgacgggct caacctgagc cgcgcctgga tgctcgaagg
     5041 gatcgcgtcg gggctaccga ccgccgaccc tcgcgtcgca gccctgcggg ccgcggcgga
     5101 tcggcatcgc aaggacggtc tcgctgccgt caccggcgcc cactacgaag gcgggcactg
     5161 gctcgggacg ttcgcgacct acctcacgac gggacgcggc ctccaggatc gctgacgcga
     5221 cggtcggccg tcagcggggc ctgcggggaa cctcgatgcg cgcgacggcg agcgtgccgt
     5281 tcccggaggc gtcggaggcc atgtacatga gatcgtagaa gcgtccgggt ccggagccgg
     5341 tgcgctccgc ccgcagcagc aggtccgcgt cggccgtgcc gatttgcgcg tcgccgacgt
     5401 cgccggccgt tgagccgtcg ctcgcgccgg tcgctgcgtc ggcctcgctg ctggtgaccg
     5461 cgacgagggc caccgcggcg ccggcatcgc agcgatcgga catcgtccag gtcaggtgca
     5521 ccggccgcag tgtgtggttc ggcggccaga gagtcgtcgg agtcgcctgg agggcgaggg
     5581 ccggcggcat cgtgtcacgc accgtgacgg tgaacggctc ggtgtcgctc tcccccccgg
     5641 cgtcggtgac gcgcagcgcc agctggtgcg tgcccagcgg cagcgtgacg ctcaacacgg
     5701 ccccggtccc caggagtgtg tcggccggtg tccaggggtc ctgccgccat tcgaacgtca
     5761 acgatccggg cgttccggcc gacgcgtccg ggtccgacga tgcggacccg tcgagggtga
     5821 cgagcgcccc gtccggcgat tcgcactcga acgtcccggc cccggcgacc gccgccgcgg
     5881 gccgcccgtc gaagcgcagt tcgtcctcac cgccgtggag gaattccacc cgcgccgcga
     5941 cgggcggcgt cttcccgtcc gaggcggaca gatcgatcac gtagggactc ccctcgatga
     6001 gcgccgacaa ttcgatgcgc gagggcagcc gatcggcgaa cggcacatcg acgaccaccg
     6061 cctgcgggtc cagcaggcgc agatcgatcg cggcctcgtc aaaccggatg accgtcagct
     6121 cgaatccggt gccgcccccc acggcgcgtc gcacgcagat cgtcgcgggc agagtcagcc
     6181 cttcgaggga caggatgggt tcgaaaggaa tcccgttccc ggcgcactcc ccgggggcca
     6241 ggagatagtc cggggcgccg tcgtcgcagg cgaagatggc gtcgagatcg aagaggaacg
     6301 gagaccccac cgccccgttg gcgaagccga accccgcgcc cggaacgccg tccacgaagt
     6361 acccgtccgc gcagtcgtcg ctggcgaggg agtcggagag ccggatcagg gcatcgaggg
     6421 cgccggtcag gcgcaccacc ccggccaccg gctcgccctg cggctcctcg acacgcgtgg
     6481 ggagacggag cgtcccaccg tcccgcacga gggtccccac gacgaaacgc ggctgacagg
     6541 cgtcgccggc caggtcggag ttcgcgtcct cctgcccgaa gttggcggcg tcctgacagt
     6601 tgtcgcactg gtcgccgcgg ccgtcggcgt cgacgtcggc ctgaggcacg ttgaaaaccg
     6661 ccgggcagtt gtcgcaggcg tctccgatcc ggtccccgtc gcgatcgtcc tgaagcggat
     6721 tggggatggc gacgcaggaa tcgcacgcgt cccccgttcc gtcggcatcg gcatcctgct
     6781 gcccggcgtt ggggaagacg gggcagttgt cggtcacatc cggcaccccg tcacggtcgt
     6841 tatcgcgatc gacggtcagg tgcgcgacga tcgccaggag aggtcgtgcc ggatcgttgc
     6901 tctggatctg cagcgtcgcc tcgtggagac cgggcagaga cgtgccggca tcgaacgtca
     6961 cggtgatctc gacggccccc ccaggcgcca cgctgcccga cagggggaga gcccgcatcc
     7021 acgaggctcc ggaggtcacg aagaacgtca ggtcgaagtg gccgggattg gcgatgacca
     7081 tcgggagagt ggtgctgtgg ccctcgggga gggaggccag gagcgtcgcc ggggagacgg
     7141 cgagaacggg tggccggtgc accgtggcgg tgagcgggac gacgacctcc ggatcgccgg
     7201 gatcgttgct caggagccgc agggtgcctg cctggatccc ctcggcgccg gagaggaccc
     7261 ggacgctgac gtcgcgcccc tcacccgggc cgacgcgcag gctcgccggc gtggcgctgt
     7321 agagcggcga atcgatctcc agagcgacct ccaggtcctc gtttcccgca ttgtcgacgc
     7381 ggagcgttcg tgccacgggg gcatccagga acacggtccc gaagtcgagg gcggtcgccg
     7441 agacgcgcag cgcgggggcc cgcccgacgt ccacgtggaa cggcacccgc acggtcggct
     7501 cgtccgggtc gttgctgcgc acgaggaggt ggtaatcgcg ctcccccgcg ggaagcccgg
     7561 acgtgtccag cgcgacggtg agcgtgcgag cggatcccgc cggcgcctca cccgacgccg
     7621 ggacgatgga gaccggcgcc accggcagcg cgtgggcgta tgggatcatg gtctccagga
     7681 ggaaccacgc cgcctcccgc gtcgtgaaaa acgtctccag gatcgtcgtc gcgacgatga
     7741 cggatccgcg gccgaggccg gacacggcaa gggtggtccg gccgggcccg gcctcggcga
     7801 tccgcacggc ggtcggcggc aggccggtga cgtaggcgcg gctccccgcc aggaaggccg
     7861 gcgcctggat gccggcggcc agcgggtgat gtggctcgag gagggtgttg aaggtcgcct
     7921 ggagggagtg cgtcgttccg cccccgggca ggacgaacgg agacggctcg ccggcccaga
     7981 cgggcgacca ggcgtgatac tcgagacagc ccccggacgc gacataggcg tcgatctgcg
     8041 cctgacgcgc ggccagggcg gcatagtagg aggacggctg ccccccggcg aggatcacca
     8101 gccgataggc cggaagatcg gtggccgcga tctgcgtcga acggatggtg tcgtacgaaa
     8161 gccgttgcga cgacaacagc cattcgttgg cgtttctccc gtcgggctgc acgtcctgca
     8221 ccagcagcac ggacccctcc gccgccgcgg cggtgccgcg gtggatgtcg cggtccacct
     8281 cgaaggcgag cgggctcgcg ccacggttgt cgatggtcag cggccaagtg acgatccgac
     8341 cgggcgcggc cgaaacgtcc agcgagcccg gaatcacctc gatctcaggc gccgccatcc
     8401 cgtggccgcg cagggcgagg gtccgcaccg gctcgtccgg atcgttgctg gtgatcgtga
     8461 gggtcgcttc gtacgttccg gcaccacccg gagtgaaggt gacgtcgatc gcccgccgct
     8521 cgcccggaag cagggccagc gcgcctgtcc ccggagcgaa tgccgggtgg tccgatgcga
     8581 gatcgctcac ctccaaggga tcgctccccg cgttggcgat ggcgaagcgc agggcgcggg
     8641 aagcaccggc gaagaccttc ccgaaatcga tcgccgcgcc gggtcccgtg tatctcagcc
     8701 ggatggtgtg ccgattgacc ggacagacgg cctccgagta cagaccgttc tggacctgca
     8761 cgtccagccg gccatcggcg agcagcgagg cgagctccgc ctcgtccatc ggaacccgca
     8821 tctccgccgt cgcgcacgcc gctccggcgt gattcacctc ttccgtgaaa gcgctctcga
     8881 cacggaagag ggcctgctcc aggccgtcat ccccgtcgtg atcgccgatc gcgaccagat
     8941 cgatgaaccc acccccttcc ggaggaaccg gagcggcgat ggagtgcgcc gtccatgcct
     9001 tttccgtcac aaagtcgcgg ctcgattcga gcgcgacctc ctggccgctc acctcgatgt
     9061 ccggcgctcc ggccgcccgg agccggaccg ggagcgggaa ctcctccagg ttcgggtcgt
     9121 tggttcgaat caccagcgtg gcgtcgtggt tcgcggcggg gagtccggcg gcgtcgaagc
     9181 ggagggcgat gtcggcctgc tctccggcgg gaacgactcc gccggaaggc tcgacggtga
     9241 gccaggcggc gcgggtgcgg gcggcccaca cgatcgcgtt gcgcagcagg agcggcatgt
     9301 cgccggtcca gcgcccggcc tgaacgaagg cgttgaccgc cacgacgttc gccgccttga
     9361 cggcgacgag cggctccccc gaggcccagg ccgcgaggat cgaggccccc ggcgccggac
     9421 ccatgtcggg gatcagcatc cccgtagccg cggtcactcc ggccatgatc ggatgcgcgg
     9481 cgtcgaatgc tccgagcgag gaggcccgcc gcgccgcgct ctcctggacg aggggatggt
     9541 agccgtcccg ttcgaaccgg ccgggcacgg cccagcgatc gaagaacgag gccacggtga
     9601 gcaccacgcc ccctcccgcg tcggcgtaat cggcgagcac atcccccagc gcgaagcggt
     9661 cgagacaggg ctcgttcacg accaggagca ccgcgtcgta gtcgagcagg gtggcgatg
//
LOCUS       DASRIQ010000447         7361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2794921, whole genome shotgun sequence.
ACCESSION   DASRIQ010000447 DASRIQ010000000
VERSION     DASRIQ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2794921"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2162
                     /locus_tag="VFD06_10925"
     CDS             <1..2162
                     /locus_tag="VFD06_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN04082.1"
                     /translation="LTSPLTAEGTIVGTFQYMAPEQIEGTPADARTDIFAFGLLLYEM
                     ATGKRAFDGKTRASLMAAVLKETPRSIAEIAPMTPPALERLIRIAMAKEPADRWQTMH
                     DVALQLRFIAEGGSRAGLPAPVAARRRIGQQAWMGTAIALLVVSAGLGVVAWRNLPAP
                     PRVIRAHIAPPEKAAFQFVGAGLGNPAVSPDGTRLAFVVRRNDGATMVYVRPLDSIRA
                     LPLAGTEGAQSTFWSPDSRTLGFFSLGKLRKIDAGGGPPLSLADAPNPRGGTWGKDGT
                     IVFAPDQSVPLVRVSASGGPVTEVTRLDDTIGESTHRWPSFLPDGKHFLYLARVVQRD
                     EHNAIRVGSLDGKVNRLIVNGESNAHYANGYLLYGRETTLMVQPFDPDRLAFTGDPFP
                     IANDVQADFSFSQMTFSVSGQGVLVYQTGESTAGSELKWYDRSGKPQATLGERTPYFG
                     LALSRDGRQAAVTAIDIRGGAADVWVFDVARGLRTRFTFDPGADNFPVFTPDGREIFF
                     SSLRKGSKRMNLYRKAVSGSGLDQVLYESERDKFASQVTPDGQSLLFFTRGDPQTKTD
                     IWIMPLGGGEPKPLIRTEFAEQGATLSPDQRFMAYESDESGRTEVYVCNFPTPTRKWQ
                     LSMAGGEKPRWRGDGREIVYLSSENRLMSVAVTPSADDFQVGSSTTLFPIQPQRPGNI
                     YDMTPDAQRFIVNTSVVDLNAQPLTLVVPWTAEIAHR"
     gene            complement(2372..6238)
                     /locus_tag="VFD06_10930"
     CDS             complement(2372..6238)
                     /locus_tag="VFD06_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN04083.1"
                     /translation="MRAQRLLAAVLWLAPVLAWEALCAPPDAHELRFAGGTMRIVPAP
                     GSNRTASAGTLSPSAPDWFAAESPAAIAKSPHGRRYLLALAPAGLSPLDRETLAAAGA
                     EVLDYVPVRGYRLRLLPAAEHAVRGLPFLAWLGETPAHLKVSAELLRRASLEAPASAL
                     APGGPPVSEARIPLRVILAADEPAGRTLDILRGLDVMPAPSGKDGAWRVAAQVPAARL
                     RGILARLAALPEVEAIEPKRELLALNQDAVWVHQSFVGPSPQSTPIFDRGLFGCGQIV
                     ALADTGQDYGSCYFQDTVNGPPPVASCSFAPCPPAAPAPGRRKDILYYNWSGTTTGDD
                     DFCPALVGGSGHGTHTSGSMAGDSSPYVDCAGFTSAGRNGGDGQAPGAKIVVQEMGDG
                     FEYLNERGGTVWNLADVAFQSGARIHSNSWGGACHDIFGGCVPGCTLPYDSFARDADL
                     AMWAHPDLLIVASAGNSGEFCPAPLALGMPANAKSVLAVGSVGHGSAATTPSSFSSPG
                     PVFDGRLKPEIAAQGESTVSAASDASAASANCGPCSLDGTSMSAPTVAGLGALVREYY
                     TAGFYAAGTRAPAQGLQPSGALVKATLIDGAVALGPGAPEPDFESGYGRVLVGQSFAF
                     TGTSFRLRVDDHRTGIATGSVVTHAYDVAAGTPFRATLVWADHPAALNAETARVNELR
                     LEVIDPSGTVWFQTIDPGSGLPVMTSDLADPHDALNVTERLVFPSPAAGRWGVRVVGV
                     DVPMEPQPFALVVRGDLTDCPAPAAPTSPALSTPAEHQVTVTWTAVPGAAAHNVYRSL
                     GSCPGGPWVVAAAAVAGTSFTDTGLSGGTAYSFRVAAASDAAGLCESPPSACVDVVPT
                     GDCTLAPLFSGAAGISSPGAAQCALALSWAPAAPSCGSDVRYNVYRSTDPAFTPGPTN
                     RIARCVPGTSWGDSVDLASGIAYHYVVRSEDATTGHGGPCRGGNEDGNVARLSSVPVG
                     PSSLGTWRDDGGDTGQAALSPAPPWSNAPTGGLTAPRVYTAPSSAGVCSDLTSPTLSV
                     GEPGLGPVLSFATRHTLEYDPVGFFGAEGSLGQVEIATGPSFGSWTRVPLTPEYPEIV
                     EFPLNDCPTTANIDTYFSGTAPYSTYSASLANWGGSDVRLRWHLSGDYLYPGGGWWID
                     DVQVTNVLTAGVCSTAAAGPPPIPDGGAVPGAPLRVSASGPGSGAPLTVTWDAARCPA
                     TAVNLYWGTLGSGTSFTGAQCTLAPTGSATVTLPDNVWFVVAATDGGAIDGSHARDLS
                     GAERIYSGAGTVCPAITTHVTNNGCP"
     gene            6416..>7361
                     /gene="tssI"
                     /locus_tag="VFD06_10935"
     CDS             6416..>7361
                     /gene="tssI"
                     /locus_tag="VFD06_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HZN04084.1"
                     /translation="MTRRAPALFLFLFLGFATGGLRAQTVSVTTPLGGDPLIVEGFRG
                     EEGTSRLFSYTLDFAAARGRDVPFDTFLGKDVTVTVTLPNGTVRHFNGIVSRFAAGRL
                     DTRAHYQMEIAPKFWLLTRRVTSRVFEDESVPQIVARILGSIPGLAFDLRLAGVFPER
                     RYVVQHNETDFDFISRLMEDEGIFYFFQHAPGGHVMVVANSPAGAPDLPGMHVFRPGL
                     FLPPPPGSVTAWEKTQEIRSGKVTLRDHTFQVPDEDFEAVATVQESVMAGAVEHHLAA
                     GAEGLEVFDYPGDYAQRFDGIDPGGAERPAELSKIFPES"
BASE COUNT         1065 a         2606 c         2653 g         1037 t
ORIGIN      
        1 atctcaccag cccgctgacg gcggagggga cgatcgtcgg cacgttccag tacatggcgc
       61 ccgagcagat cgaagggacg ccggcggacg cgcgcaccga catcttcgcc ttcgggctgc
      121 ttctgtacga gatggcgacc ggcaagcgcg ccttcgacgg gaagacgcgc gcctcgctca
      181 tggcggcggt tctcaaggag acgccccgct ccatcgccga gatcgccccg atgaccccgc
      241 cggccctcga gcgcctcatc cgcatcgcca tggcgaagga gccggccgat cgctggcaga
      301 cgatgcacga cgtggcgctg caattgcgct tcatcgccga ggggggctcg cgggcgggcc
      361 ttccggcacc ggtcgcggcc cgccgccgca tcggacagca ggcgtggatg ggaacggcga
      421 tcgccctcct cgtcgtgagc gccgggctcg gagtggtcgc ctggaggaat ctcccggcgc
      481 cgccgcgcgt catccgggcc cacatcgcgc ctccggaaaa ggcggcgttc cagttcgtcg
      541 gcgcggggct cgggaacccc gcggtctctc cggacggcac ccgcctcgcg ttcgtggtgc
      601 gcaggaacga cggcgcgacc atggtctatg tccgtccgct cgactcgatc cgggcgctgc
      661 cgctcgccgg caccgagggg gcgcagagca ccttctggtc tcccgacagc cgcacgctgg
      721 ggttcttctc cctgggcaag ctcaggaaga tcgacgccgg cggcggcccg ccgctgtcgc
      781 tcgcggatgc gccgaatccg cgcggcggga cctggggaaa ggacggcacc atcgtcttcg
      841 cccccgacca gagcgtcccc ctcgtgcgcg tgtcggcctc gggtggtccg gtgaccgagg
      901 tgaccaggct cgacgacacg atcggcgaaa gcacccaccg gtggccgtcg ttcctgccgg
      961 acgggaagca cttcctctac ctcgcgcgcg tcgtgcagcg ggacgagcac aatgccatcc
     1021 gcgtcggctc cctggacggc aaggtcaacc gtctcatcgt caacggggaa tcgaacgcgc
     1081 attacgccaa cggctacctc ctctacggcc gcgagacgac gctgatggtg cagccgttcg
     1141 atccggatcg cctggcgttc acgggcgatc cgttcccgat cgccaatgac gtccaggccg
     1201 acttctcctt ctcccagatg acattctcgg tctccgggca gggggtgctc gtctaccaga
     1261 cgggggagtc gacggccggc tcggagttga agtggtacga ccgctccggc aagccccagg
     1321 cgaccctggg ggagcggacc ccctacttcg ggctggccct gtcgcgtgac ggccggcagg
     1381 ccgccgtcac ggcgatcgac atccgcggcg gcgccgccga cgtctgggtc ttcgacgtgg
     1441 cgcggggact ccggacccgg ttcaccttcg acccgggggc cgacaacttc ccggtcttca
     1501 cccccgacgg cagggagatc ttcttctcct ccctgcgcaa ggggagcaag cggatgaacc
     1561 tgtaccggaa agcggtctcc gggtcgggac tggatcaggt cctgtacgag tcggagcggg
     1621 acaagttcgc gagccaggtg acgcccgacg gccagtcgct gctcttcttc acccggggcg
     1681 acccgcagac gaagaccgac atctggatca tgccgctcgg tggcggcgag ccgaagccgc
     1741 tcatccggac cgagttcgcc gagcaggggg cgaccctgtc ccccgaccag cgcttcatgg
     1801 cgtacgagtc ggacgaatcg gggcggacgg aggtgtacgt ctgcaacttc cccacgccga
     1861 cgcgcaagtg gcagctctcc atggcgggcg gcgagaagcc ccgctggcgc ggcgacggcc
     1921 gggagatcgt ctatctctcc tccgagaaca ggctcatgtc ggtggcggtg acgccgtcgg
     1981 ccgatgattt ccaggtcggg tcgtccacca ccctgttccc gattcagccg cagcgtcccg
     2041 gcaacatcta cgacatgacc cccgacgcgc agcgcttcat cgtcaacacg tcggtcgtgg
     2101 acctcaacgc ccagccgctg accctcgtcg ttccctggac ggcggagatc gcgcacaggt
     2161 agcacggcgg gatgtcctga attgccctcc cgggaactgc tgacagtaga tcggcgcatt
     2221 gccaggatcg gcagcgcggt gctaacagtg ttgctcgcag gtgcttttcg atcgggccgg
     2281 gtcctttctc tccctccagc ccgttcccgg tcgacctgag gtgcccggat gccggcgatc
     2341 acggcccgcc cgcgtccggg cacacgttgt ttcagggaca gccgttgttg gtgacgtgcg
     2401 tggtgatcgc gggacagaca gtcccggcgc ctgaatagat tcgctccgcc cccgaaagat
     2461 cgcgcgcgtg gctgccgtcg atcgcaccac cgtcggtggc ggcgacgacg aaccagacgt
     2521 tgtcggggag tgtgacggtc gcgctcccgg tcggcgccaa ggtgcactgg gcgccggtga
     2581 acgaggtccc gctcccgagc gtcccccagt agagattcac cgccgtcgcg gggcagcgcg
     2641 ccgcgtccca ggtgacggtg agcggcgcgc ccgagccggg acctgacgcc gacacgcgca
     2701 gcggcgcgcc gggaaccgcg ccgccgtccg ggatcggtgg cggaccggcg gccgccgtgg
     2761 agcacacacc ggcggtcagc acgttcgtca cctgcacgtc gtcgatccac cagccgcctc
     2821 cgggatacag gtagtcgccg gacagatgcc agcgcagccg cacgtcgctg ccgccccagt
     2881 tggcgagcga cgcgctgtag gtcgagtacg gcgcggtgcc gctgaaatag gtgtcgatgt
     2941 tcgccgtcgt cgggcagtcg ttgagcggga actcgacgat ctcggggtac tcgggcgtca
     3001 acggcacacg cgtccagctg ccgaacgacg gccccgtggc gatctccacc tggccgagcg
     3061 acccctcggc gccgaagaag ccgaccgggt cgtactcgag cgtgtgccgc gtcgcgaacg
     3121 agaggaccgg tccgagcccc ggttcgccga cgctcagcgt cgggctcgtc aggtcggagc
     3181 acacccccgc gctgctcggc gcggtgtaga cgcgcggcgc cgtcaggcct ccggtcggcg
     3241 cattgctcca gggcggcgcg ggggagaggg ccgcctggcc ggtgtcgccg ccgtcgtcgc
     3301 gccaggttcc cagggagctc ggtccgaccg gcaccgagga gagccgggcc acgttcccgt
     3361 cctcgttgcc gccgcggcac ggcccgccgt gaccggtcgt cgcgtcttcg gaccgcacga
     3421 cgtagtgata ggcgatgccg ctcgcgagat cgacgctgtc gccccacgag gtccccggga
     3481 cgcagcgggc gatccggttc gtgggtcccg gcgtgaacgc cgggtcggtg ctccggtaga
     3541 cgttgtagcg gacatcgctg ccgcaggagg gggcggccgg tgcccaggag agcgccaggg
     3601 cgcactgggc ggctccggga ctcgagatcc ccgcggcccc ggagaagagc ggtgccaggg
     3661 tgcagtcgcc cgtgggaacg acgtcaacgc acgccgacgg cggcgactcg caaagccccg
     3721 cggcatccga ggctgcggcg actctgaagg aatacgccgt cccgcccgag agcccggtgt
     3781 ctgtgaacga cgtcccggcg acggccgcgg cggcgaccac ccacgggcct cccgggcacg
     3841 atccgaggct gcggtagacg ttgtgcgcgg cggcgcccgg caccgcggtc caggtcacgg
     3901 tcacctggtg ctcggcgggc gtggacagcg ccggagaagt gggggccgcc ggagccgggc
     3961 agtcggtgag atcgccgcgc acgacgaggg cgaacggctg cggctccatc ggaacgtcca
     4021 ctccgacgac ccgcaccccc cagcgtcccg cggccgggct cgggaagacg agccgctccg
     4081 tgacgttgag cgcatcgtgc gggtccgcca ggtcgctcgt catcaccggc agaccgcttc
     4141 cggggtcgat cgtctggaac cagacggtcc cggaggggtc gatcacttcg agccgcagct
     4201 cgttgacccg cgccgtctcg gcgttcagcg ccgccgggtg gtcggcccag acgagggtgg
     4261 cgcggaacgg cgtgccggcc gcgacgtcgt acgcgtgcgt cacgacgctg ccggtcgcaa
     4321 tcccggtccg gtggtcgtcg acgcgcaacc ggaacgacgt gcccgtgaag gcaaacgact
     4381 ggccgacgag cacccggccg tagcccgact cgaaatcggg ctcgggcgcc ccgggaccga
     4441 gtgccacggc gccgtcgatg agggtcgcct tgacgagtgc gccgctcggc tggagtccct
     4501 gcgccggggc gcgcgttccc gcggcgtaga agccggcggt gtagtactcg cgcacgagcg
     4561 cgccgagccc cgcgacggtc ggcgcggaca tcgacgttcc gtccagcgag cagggcccgc
     4621 agttggcgct cgccgcgctg gcgtcggagg cggccgagac cgtgctctct ccctgcgccg
     4681 cgatctccgg cttgaggcgg ccgtcgaaga ccggcccggg gctggagaac gacgacggcg
     4741 tcgtggcggc cgagccgtgc ccgaccgagc cgacggcgag gacgctcttg gcgttcgccg
     4801 gcatccccag agcgagcggc gcggggcaga actccccgga gttgccggcc gacgcgacga
     4861 tcaggaggtc ggggtgcgcc cacatcgcga ggtccgcatc gcgcgcgaac gagtcgtagg
     4921 gcagcgtgca ccccggcacg caccccccga agatgtcgtg gcaggcgccg ccccacgagt
     4981 tcgagtggat gcgcgccccg ctctggaacg cgacgtcggc gaggttccag accgtcccgc
     5041 cgcgctcgtt gaggtactcg aagccgtccc ccatctcctg cacgacgatt ttggccccgg
     5101 gcgcctggcc gtcgccgccg ttacgccccg ccgaggtgaa cccggcgcag tcgacgtacg
     5161 gcgaggagtc cccggccatc gacccggagg tgtgcgtgcc gtgcccgctg ccgccgacga
     5221 gggcggggca gaagtcgtcg tcgccggtcg tcgtcccgga ccagttgtaa tagaggatgt
     5281 ctttgcgtcg ccccggcgcg ggggcggcgg gggggcaggg ggcgaaggag caggaggcga
     5341 ccggcggcgg gccgttcacc gtgtcctgga agtagcagga gccgtagtcc tgcccggtgt
     5401 cggcgagcgc cacgatctga ccgcagccga acaggccgcg atcgaaaatc ggcgtggact
     5461 gcggcgacgg cccgacgaac gactggtgca cccagacggc gtcctggttg agcgcgagca
     5521 gctcgcgctt cggctcgatc gcttccactt cgggaagcgc cgccagccgc gccaggatgc
     5581 cgcgcagccg cgccgccggc acctgcgccg cgacgcgcca ggcgccgtcc ttgcccgagg
     5641 gggccggcat cacgtcgaga ccgcgcagga tgtcgagcgt ccgccccgcc ggttcgtcgg
     5701 cggcgaggat gacgcgaagc gggatccgcg cttcggacac gggcggccct ccgggcgcga
     5761 gcgccgacgc aggggcttcg agcgaggcgc gccgcagcag ctccgcagag accttgaggt
     5821 gcgcgggggt ttcgccgagc caggccagga agggcagccc ccgcacggca tgctccgcgg
     5881 ccggaagcag tcgcagccgg tatccgcgca ccgggacgta gtcgaggacc tccgccccgg
     5941 ctgccgccag cgtctccctg tccaggggac tcaggccggc cggagccagg gcgagcaggt
     6001 accgccggcc gtgcgggctc ttcgcgatcg cggcggggga ctccgcggcg aaccagtcgg
     6061 gggcggacgg gctcagcgtc ccggcggatg cggtgcggtt cgatcccggt gcgggaacga
     6121 tcctcatcgt cccgcccgcg aagcgcagct cgtgggcgtc cggcggggcg cacaaagcct
     6181 cccacgccag gacgggcgcg agccacaaca cggcggcgag caagcgttgt gcgcgcatgg
     6241 agcgagcggt tgggcgaatc ataacctcgg cccccgggcc tggagcccag aacgggggtc
     6301 ccgattcgat ccggggcgcc tcctgaatcc cgcccggccg ggccccagaa tcccgttgac
     6361 cggccggggc gtccgtccca cattccggag gcgttccgca tcgatggagg gagccatgac
     6421 gagacgcgcc ccggcgctgt tcctgttcct cttcctgggc ttcgcgaccg gcggacttcg
     6481 agcccagacc gtgtcggtga cgacgccgct cggaggcgat ccactcatcg tggaaggctt
     6541 ccgcggggag gaggggacct cgcggctctt ctcctacacg ctcgattttg cggcggcgcg
     6601 ggggcgcgac gttccgttcg acacgttcct cgggaaggac gtcacggtga ccgtgacgct
     6661 gccgaatggc acggtgcgcc acttcaacgg catcgtctcc cgcttcgccg ccggccggct
     6721 cgacacccgg gcgcactacc agatggagat cgcgccgaag ttctggctcc tgacgcgccg
     6781 ggtgacgagc cgcgtcttcg aggacgaatc ggtgccgcag atcgtggcgc gcatcctcgg
     6841 gagcatcccc gggctcgcgt tcgacctgcg cctggccggc gtcttcccgg agcgccgcta
     6901 cgtggtgcag cacaacgaga ccgacttcga cttcatcagc cgcctgatgg aggacgaggg
     6961 gatcttctac ttcttccagc acgcgccggg cgggcacgtc atggtcgtgg ccaactcgcc
     7021 ggccggagct cccgacctgc cggggatgca cgtctttcgg cccggtctct tcctgccgcc
     7081 gcctcccggg tcggtgaccg cctgggagaa gacgcaggag atccgctcgg ggaaagtgac
     7141 cctgcgcgac cacacgttcc aggtccctga cgaggatttc gaagcggtgg cgacggtcca
     7201 ggaatcggtg atggccggcg ccgtggagca tcatctcgcc gccggcgccg aggggctgga
     7261 ggtgttcgac tacccgggtg actacgcgca gcgcttcgac ggcatcgacc cgggcggcgc
     7321 cgagcggccg gcggagctgt cgaagatctt tcccgaaagc c
//
LOCUS       DASRIQ010000448         8579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_714629, whole genome shotgun sequence.
ACCESSION   DASRIQ010000448 DASRIQ010000000
VERSION     DASRIQ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714629"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFD06_10940"
     CDS             <1..924
                     /locus_tag="VFD06_10940"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN04085.1"
                     /translation="DLASLTKPLATALLAVRLQSAGRLRLEDRVDRHLPEWRPAEDGP
                     PLTLLDLLTHRSGLPAWLPLYARAEDVTGRIGFVAGTAPAHPPLLGVTYSCPNYLLAG
                     FAIERVAGAPLDRLFETLGTGPLGIEDLGYRPHPGLRDRIAPTETGNAHERALAGPLA
                     AGYNGWRRDLIWGEVHDHNAWSLGGVAGNAGLFGTARAVWSLARVFLRGGPGLLGDEE
                     RALFGHDFTPGLNQARAVGFQLAATPGCSAGPALSPRSFGHTGFTGTSLWIDPDAERI
                     AVLLTNRVHPVHRERDLHPLRREFNALARAL"
     gene            953..2119
                     /locus_tag="VFD06_10945"
     CDS             953..2119
                     /locus_tag="VFD06_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZN04086.1"
                     /translation="MDILSLNEPPQVPARRLSRAEAIGASMVLHVLVLLLFLRGLEGL
                     PEPIARWLSAHAAHPPASEAAQNVTPLPTPATPDEKKQEEERKKAEPIPLKFAYVKIP
                     EEAQPQKSEDATHFSDRDRTARQPVPTPPDATRFTRDPHAEGDTRERVVPDPRVKPGK
                     DQLEPPQPETERIAAKDVLGREKEADGAGGSAGKTGEEGTPSEESSGREGHDALPPEA
                     NGRGQAVLPGPAGSQRTPSGGGGPAGGPFASRRPDLRESTEQKFHFENTGWMRGETVD
                     GNLSFDTKGLPWGDYARKIHVIIRQNWIDRLPLAFRQGQRGFTCQHFVIERDGTIRDI
                     DVLKQSRVPPFDRAASDALKASSALPPLPPEFNLESEGVTFCFYYNMYWIEEDE"
     gene            complement(2132..6058)
                     /locus_tag="VFD06_10950"
     CDS             complement(2132..6058)
                     /locus_tag="VFD06_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN04087.1"
                     /translation="MPQLRRRPTAPVVIAATVLFGVFAGPVPEARPLFQPRPGPIDHP
                     LAIAAADFDRDGRDDLAFAAYQAGVVEILLGQANGTFLPSAESPVGVGTATFSTPTTG
                     PQGLIVFDLNPQDVDGDTIPNASDNCPNVPNGPEATEGSQIDANTNGVGNACEAGEDT
                     DDDGDVDVTVDTDGDGVLDFDPVTFRLDNCPRRPNPGQEDTETAQGPDGMCGTIDDNT
                     LLYGTDALCGTTDDRTGDRVGDACAASPDLLVLTNSVGGGSTLGTVRVRVNNGTGGFL
                     NRPSYVTGVGPGQAILADVSGDRLVDLLIANVSIDLVQVFLGIGDAQFTGGSLLTAGD
                     GPQAIVAADIDNDGDLDPITADSAAGTLSLFRNAPGALGPSPSETFTTGTHPAVLLSG
                     NLNGDAFADIVVLTQGALTCTGPPMGTLTGRACTIDKDCDDLFTIGQDGTCSGGDGGV
                     QVFAGAPASPPPGLVAGPLVSFGPGHRPRAAKLRDLNGDTFLDLAVADFAGGEILILP
                     GNGDGTFGTAAVLPINGQPGDLAFLTLDPATPSDLAVLDTAGRRIDLFEQTAGLSYAA
                     APVTPASPWRDTRGMALIAADGLVAFDVVLLQQQGARVDVLSGIGDGSFRVTPAVRPE
                     GAANGSGFVTADLRQDGRPDLAIFDSVAGTVTVFTGELNGLLEERGTVSAGAGAARAD
                     ATPLYESIVDYDRDGVPNVLDDCPTVYNPPMCKTTDPLCTPVIPFVCTDDVLAPNDCD
                     PAAIPPQLDPVTGQCDSDRNGIGDLCQVLGAAEATTGTCFAVDSDFDLKADYDHKALK
                     KDVNGALDFDGDTFANTVDNCPTMANVDQKDGPPANGIGDACEVLGSGQTVDPDGDLV
                     PTWNPITQAVDNCPLIPNKGQEDNDGDRVGNACIVAAALDNCLGTFNANQQDGDGDGL
                     GDACAVPPQDLFLLSPDPPGAIAVLQGDGSGTLRDSPISPLTGLSGPLAVRSGHFSLA
                     CSPQGAFCSDKSELHIFDLAIAEAGTLGDPSDDAIALRQGDGAGGFTALPAFPVTGDP
                     SLILRAQDQPVCPIASSPTNPQLRFDPDVQTDLLVALGTADSTLHVLLPSNQNYLNAA
                     LSPLVRPVAFDAPLPVTGSLRDVVLANVNQDALQDLVAISTSGGTTTITPFIGLGNGL
                     FFTDPGLVTTNLPFDASRLQSANVDIKTDVVYPDLVLFESRDEAPFSLLNTLPERADI
                     DSSGRVDGYDLALLAASFGAARGEDFTLLPDATFARTGSGATEVIVRTGSAVPGQDLP
                     DSAGACNIAFDAGSGSYGVPVDINLDGIVDGEDLAFLASRFGTTLR"
     gene            6216..6785
                     /gene="rsmD"
                     /locus_tag="VFD06_10955"
     CDS             6216..6785
                     /gene="rsmD"
                     /locus_tag="VFD06_10955"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZN04088.1"
                     /translation="MAGLRILGGAARGRRLQGESLPGLRPTSARVREALFDVLGARVT
                     GCTFLDLYAGTGAVGLEALSRGAARAVFVESSRRAVRALAANLKVLSETGAGDILEMD
                     AGQALYRLEERGERFGVVFLDPPWDLDDGGLILQQAARAVAPSGVLVVEHRTGRPPSV
                     APDSGLTPGRAYRHGDASLSLFLAGASSR"
     gene            6782..>8579
                     /locus_tag="VFD06_10960"
     CDS             6782..>8579
                     /locus_tag="VFD06_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04089.1"
                     /translation="MMLRRLLGGLVLVLTAAFCLTPLAEVDFFWHLLAGERILADGRV
                     PRVDAFTFTSSGRPWIDLQWLFQTGAAAAHAAGGFPLLDALKTAILTSAFALALLTAS
                     RRRATLAAPILALPGLVAAQERFTLRPEMLSFLFLALLLLVLGERRRHPRLLFLLPPL
                     FALWANAHSLYAAGLGAAILVLIGDALEGRPRGETTRLGGVALLSAAATLLTPYGLRG
                     WALLRTLLFERVSGETLFARRIAEFQSPFGGSGATASVAGLAILIVLILVISFAGRRA
                     MAPADRLLLLAFTGLALLARRNMPLLALVAVPCAAPAAASAWEALTRRLRTGAPPAGR
                     SRERILSGIAGCACGVAAAALLADVVSNRFYARDGTQRSFGTGLAPAVFPEITAGLVE
                     RLRLEGEAFHDLADGGYLAWRWWPDRRVYIDGRLEVHPESLFAIWLQALSDPVRFEAE
                     ARARGIGLVVWSHRSAVDAAPLLRHLATSPSWRLVHLDLESALFLRREAGGGPEVASD
                     SDLEAPALRLLAEAEAAAARARDQDPAPGWLRRILPRLDIPTGETGAALFLALTGRPG
                     SAVALFEDACRRAPWSAPLRYDLGLARAQAGDD"
BASE COUNT         1151 a         2996 c         3106 g         1326 t
ORIGIN      
        1 gacctcgcct ctctgacgaa accgctcgcg acggcgctgc tcgccgtccg gctgcagtcg
       61 gcggggaggc tccggctcga ggatcgcgtc gatcgccacc tgccggagtg gcgcccggcc
      121 gaggacggac cgccgctcac gctcctcgat ctgctcacgc accgatccgg gctgccggcc
      181 tggctgccgc tctacgcccg cgcggaggac gtgaccggac ggatcggttt cgtcgccggg
      241 acggcgccgg cgcatccgcc gctcctcggc gtgacctact cgtgtccgaa ctacctcctg
      301 gccgggttcg cgatcgagcg ggtggcaggc gcccccctcg atcgcctctt cgagacactc
      361 gggaccggcc ctcttggcat cgaggatctc ggctatcgcc cccacccggg gctgcgggat
      421 cggatcgcgc cgaccgagac cggcaacgcc cacgagcgcg cgctcgccgg ccctctggcc
      481 gccggataca atggctggcg gcgggacctg atctggggcg aagtgcacga tcacaatgcg
      541 tggagtctcg gcggggtcgc cggcaatgcg ggcctgttcg ggacggcgcg ggcggtgtgg
      601 tccctggcgc gcgtctttct gcgcggcggg cccgggctcc tgggagacga ggagcgggcc
      661 ctcttcgggc atgacttcac cccgggcctg aaccaggcgc gcgcggtggg ctttcagctc
      721 gcggccacgc cgggctgctc cgccgggccg gcgctgtcgc cgcgctcctt cggtcacacc
      781 gggttcaccg gcacctccct gtggatcgat ccggacgcgg agcggatcgc ggtgttgctg
      841 accaaccgag tccatccggt ccaccgcgag cgcgacttgc acccgctccg gcgcgagttc
      901 aacgccctgg cgcgagcgct ctgatcgcct ctccgggagg gccgagcacc gcatggacat
      961 cctgtccctc aacgagccac cgcaggtccc ggcacggcgc ctgtcgcgcg ccgaggcgat
     1021 cggcgcgtcg atggtcctgc acgtcctggt gctgctcctg ttcctgcgtg gtctggaggg
     1081 acttcccgag ccgatcgccc ggtggctgag tgcgcacgcc gcccaccctc cagcgtccga
     1141 ggcggcccag aacgtcacgc ccctgccgac ccccgcgacc cccgacgaga agaagcagga
     1201 ggaggaacgg aagaaggcgg agccgattcc cctgaaattc gcctacgtca agatccccga
     1261 ggaggcgcag ccccagaaga gcgaggacgc cacccacttc tccgatcgcg atcggacagc
     1321 gcgccagccg gtgccgaccc ccccggacgc gacccggttc acgcgcgacc cgcacgcgga
     1381 gggggacacg cgcgagcgcg tcgtccccga cccgcgcgtc aagccgggga aggaccagct
     1441 ggaaccgcct cagccggaga cagaaaggat cgctgcgaag gacgtcctcg gccgcgagaa
     1501 ggaggccgac ggcgccggag ggagcgcggg gaagacgggt gaggaaggca cgccttccga
     1561 ggaatcgtcc ggacgcgagg gacacgatgc gctccctccg gaagcgaatg gccgtgggca
     1621 ggcggtcctc ccgggtccgg ccgggagcca gcgcaccccg tccgggggag gagggccggc
     1681 gggggggccg ttcgcctccc ggcggcccga tctccgcgag agcaccgaac agaagttcca
     1741 cttcgaaaac acgggatgga tgcgcggaga aaccgtcgac ggtaacctgt cgttcgacac
     1801 gaaggggctg ccgtgggggg actacgcgcg gaagatccac gtgatcatcc gccagaactg
     1861 gatcgaccgg ctgccgctcg ccttccgaca gggtcagagg ggattcacct gccagcactt
     1921 cgtcatcgag cgcgatggga cgatccgcga catcgacgtc ctcaagcaat cgcgcgtccc
     1981 gccgttcgat cgggcggcct ccgatgccct caaggcgagc agcgcgctgc cgcctctccc
     2041 gccggagttc aacctcgaaa gcgaaggggt gaccttctgc ttctattaca acatgtactg
     2101 gatcgaggaa gacgagtgac gcgggagcag ctcagcgcag cgtggtgccg aagcggctcg
     2161 cgaggaaggc cagatcctcg ccgtcgacga tcccgtcgag gttgatgtcc accgggacgc
     2221 cgtaggaccc gctgccggcg tcgaaggcga tgttgcacgc cccggctgag tcggggagat
     2281 cctgtccggg gaccgccgac ccggtccgga cgatgacctc ggtcgcgccg cttccggtgc
     2341 gggcgaaggt ggcgtccggc agaagcgtga aatcctcccc gcgcgccgca ccgaacgaag
     2401 ccgccagaag agcgagatcg tacccgtcca cgcgtcccga gctgtcgatg tcggcgcgct
     2461 ccggcagcgt gttgaggagc gaaaaggggg cttcatcgcg cgactcgaac aggacgaggt
     2521 ccggatacac gacgtcggtc ttgatgtcga cgttggcgct ctggagacgg ctcgcatcga
     2581 agggcaggtt cgtcgtcacg agtcccggat cggtgaagaa caggccgttg ccgagcccga
     2641 tgaacggggt gatggtcgtc gtcccgccgc tcgtggagat cgccaccagg tcctgcaggg
     2701 cgtcctggtt gacgttcgcc agaacgacgt cgcgaaggct ccccgtcacc gggagcggcg
     2761 cgtcgaacgc caccggacgc accaggggag acagggcggc gttgaggtag ttctggttgc
     2821 tcgggaggag gacgtgcagc gtcgagtcgg cggtcccgag cgcgaccagc aggtcggtct
     2881 ggacgtcggg gtcgaagcgc agctgcggat tcgtcgggct cgaggcgatc gggcagaccg
     2941 gctgatcctg ggcccgcagg atcagggacg ggtctcccgt gaccggaaag gccggcaggg
     3001 ccgtgaatcc accggcaccg tccccctggc gcagggcgat ggcgtcgtcc gacggatcgc
     3061 ccagggtccc ggcttcggcg atggcgagat cgaagatgtg gagctcgctc ttgtcgctgc
     3121 agaaggcccc ctgggggctg caggcgagcg agaagtggcc ggagcggacg gccaaggggc
     3181 ccgacagtcc ggtcagcggc gagatggggc tgtcccggag cgtgcccgat ccatcccctt
     3241 ggaggaccgc gatcgcgccg ggcggatccg ggctcaaaag gaacagatcc tgggggggca
     3301 cggcgcaggc gtcaccgagg ccgtcgccgt cgccgtcctg ctgattcgcg ttgaaggtcc
     3361 cgaggcagtt gtccagcgcc gcggcgacga tgcaggcgtt cccgacccgg tcgccgtcgt
     3421 tgtcctcctg tcccttgttg ggaatgagcg ggcagttgtc caccgcctgg gtgatcggat
     3481 tccaggtggg gaccaagtcg ccgtccggat cgacggtctg gccggagccg aggacttcgc
     3541 aggcgtcgcc gataccattt gcgggcgggc cgtccttctg atcgacgttc gccatcgtgg
     3601 gacagttgtc gaccgtgttc gcgaacgtgt ctccgtcgaa atcgagcgcc ccgttcacgt
     3661 ccttcttgag ggccttgtgg tcgtagtcgg ccttgaggtc gaaatcggag tcgacggcga
     3721 agcaggtgcc ggtggtcgcc tccgccgccc ccagcacctg gcagaggtcg ccgatgccgt
     3781 tgcgatccga atcgcactga cccgtgaccg ggtccagctg cggcggaatg gcggccgggt
     3841 cgcagtcatt gggggccagg acgtcgtcgg tgcacacgaa cggaatgacg ggggtgcaga
     3901 gcggatcggt cgtcttgcac atcggcgggt tgtagaccgt ggggcagtcg tcgaggacgt
     3961 tcggcacccc gtcccggtcg tagtcgacga tcgattcgta gagcggcgtc gcgtccgccc
     4021 gggccgcgcc ggcgccggcg ctgacggtgc cgcgctcctc gagcaggccg ttgagctctc
     4081 cggtgaacac cgtcacggtg cccgcgacac tgtcgaagat cgccaggtcg ggccggccgt
     4141 cctgccgaag gtcggccgtg acgaatccgc tcccgtttgc cgccccctcc ggccgcaccg
     4201 ccggcgtgac gcggaacgag ccgtcgccga tgccgctcag gacgtcgacg cgagccccct
     4261 gctgctgcag gaggacgacg tcgaacgcga ccaggccgtc cgcggcgatg agcgccatgc
     4321 cgcgggtgtc gcgccagggg gaggccgggg tgacgggggc ggcggcgtag gagagtcccg
     4381 cggtctgttc gaagagatcg atgcggcggc cggccgtgtc gagcacggcg agatccgacg
     4441 gtgtggccgg gtcgagcgtc aggaaggcga ggtcgcccgg ctgtccgttg atcgggagga
     4501 cggcggcggt cccgaaggtc ccgtcgccgt tcccgggaag aatcaagatc tcgccgccgg
     4561 cgaaatcggc cacggcgaga tcgaggaagg tgtccccgtt cagatcgcgc agtttcgccg
     4621 cgcgtggccg gtgccccggc ccgaagctca cgagcggccc cgcgacgagc ccgggcggcg
     4681 ggctggccgg ggcgccggcg aagacctgca cgcccccgtc cccccctgag caggtgccgt
     4741 cctgcccgat cgtaaatagg tcgtcgcaat ccttgtcgat ggtgcaggcc ctgcccgtga
     4801 gcgtccccat gggcggcccg gtgcacgtga gcgcgccctg cgtcagcacg acgatgtcgg
     4861 caaaggcgtc gccgttgagg tttccggaca acaggacggc ggggtgggtg ccggtcgtga
     4921 acgtctcgct cggggagggg cccagcgcgc cgggggcgtt gcggaagagg gacagggtgc
     4981 cggcggcgct gtcggcggtg atcggatcga ggtcgccgtc gttgtcgatg tcggccgcga
     5041 cgatcgcctg cggaccatcc cctgccgtca aaagcgaccc gccggtgaac tgcgcgtcac
     5101 cgatgccgag gaacacctgc acgagatcga tgctgacgtt ggcgatcagg agatcgacca
     5161 gccgatcgcc gctgacgtcc gcgagaatcg cctggcccgg ccccacgccg gtcacgtagg
     5221 acgggcggtt caggaagccg ccggttccgt tgttcacccg cacccggacc gtcccgagcg
     5281 tcgagccgcc ccccaccgag ttggtcagga cgagcagatc ggggctggcc gcgcaggcgt
     5341 cgccgactcg gtcgccggtt cggtcgtcgg tggtgccgca gagagcgtcg gtcccgtaca
     5401 ggagggtgtt gtcgtcgatt gtgccgcaca tcccgtccgg cccctgggcc gtctccgtgt
     5461 cctcctggcc gggattcgga cgccgcggac agttgtcgag ccggaacgtc accggatcga
     5521 agtcgagcac cccgtcgccg tcggtgtcga ccgtgacgtc gacgtcgccg tcgtcgtcgg
     5581 tgtcctcgcc ggcttcgcag gcgttcccga cgccgttcgt gttggcgtcg atctgcgagc
     5641 cttcggtcgc ctcggggccg ttggggacgt tcggacagtt gtcgctggcg ttcggaatcg
     5701 tgtcgccgtc gacgtcctgc gggttcaggt cgaagacgat cagtccctgc ggtccggtgg
     5761 tcggagtcga gaaggtggcc gtgccgactc cgacggggct ttcggcgctc gggagaaaag
     5821 tgccgttcgc ctgtccgagc aggatttcga ccactcccgc ctgataggcg gcgaaggcca
     5881 ggtcgtcgcg gccgtcccga tcgaaatcgg ccgcggcgat cgccagcgga tgatcgatcg
     5941 gccccgggcg cggctggaac agcggccggg cttcgggaac ggggccggcg aacacaccga
     6001 aaaggacggt cgcggcgatg acgaccggcg ccgtcggccg ccggcgtagc tgaggcatcg
     6061 atctcctgct ggagcccccc cgggccgcgg aatgctactg gccaggtgcg ggcaagtcaa
     6121 gaaaaggaag gggcgtggcc gcggtgtata gtccccttcg tgagcagcct cgggcgcgcc
     6181 cacacgagcg agggcattgg cagcgccggc cggtcgtggc cgggctgagg atcctcggcg
     6241 gtgcggcgcg cggccgccga ttgcaggggg agtcgctccc ggggctgcgc ccaacctcgg
     6301 cccgcgtccg cgaggccctc ttcgacgtcc tcggcgcgcg cgtgaccggg tgcaccttcc
     6361 tcgatctcta cgccggcacg ggggccgtcg gcctcgaggc cctgagccgg ggcgcggccc
     6421 gcgccgtctt cgtcgagtcg agccgtcgcg ccgtccgggc gctcgcagcc aatctgaagg
     6481 tcctctcgga aaccggcgcg ggggacatcc tcgagatgga cgccgggcag gcgctttacc
     6541 gactcgagga gcggggtgag cgcttcggcg tcgtctttct cgatcctccc tgggacctcg
     6601 acgacggcgg cctcattctg caacaggccg cgcgcgcggt ggctccctcc ggagtgctgg
     6661 tcgtcgagca tcgcaccggc cgtcctccgt cggtcgctcc cgactcggga ctgacgcccg
     6721 gtcgcgccta ccggcacggg gatgcgtcgc tgagcctgtt cctggctggc gcttcctccc
     6781 gatgatgctc cgacgattgc tcggcgggct cgtgctcgtc ctgactgccg cgttctgcct
     6841 gaccccgctc gccgaggtcg atttcttctg gcacctcctg gcgggggagc gcatcctcgc
     6901 cgatggccgc gtcccccgcg tcgacgcctt caccttcaca tcgtccgggc gtccctggat
     6961 cgacctgcag tggctcttcc agaccggcgc cgccgccgcg cacgcggccg gcggcttccc
     7021 gctcctcgac gcgctcaaga cggccatcct gacgtctgcc ttcgccctgg cccttctcac
     7081 cgcgtcgcgt cgccgcgcca ccctggcggc gccgatcctg gcgctcccgg gtctcgtcgc
     7141 cgcgcaggag cgtttcacgc tgcgtccgga gatgctgtcg ttcctgtttc tggcgctgct
     7201 cctgctggtt ctgggggagc gccggcgaca tccgcggctg ctgttccttc tcccgcccct
     7261 tttcgcgctg tgggcgaacg cccacagcct ttacgccgcg ggtctgggag cggcgatcct
     7321 ggtcctgatc ggcgatgctc tggagggccg gccgcgcggc gagaccacgc ggctcggcgg
     7381 ggtcgccctt ttgtccgccg ccgcgaccct tctgaccccg tacggtctgc gcggatgggc
     7441 ccttctgcgg acgctcctgt tcgagcgggt gtcgggggag acgctgttcg cgcggcgcat
     7501 cgcggaattc cagtcccctt tcgggggatc gggcgcgacc gcctcggtcg ccgggctcgc
     7561 catcctgatc gtcctgatcc tggtgatctc tttcgccggc cggcgcgcca tggcccccgc
     7621 ggaccgcctg ctccttctgg cgttcaccgg tctcgccctg ctcgcccgcc gcaacatgcc
     7681 gcttctggct ctcgtcgccg tcccgtgcgc cgcgccggcg gccgcgtcgg cctgggaagc
     7741 gctcacgcgt cgcctgcgca cgggcgctcc gccggccggc cggtcgcggg agcgcatcct
     7801 cagcggcatc gcggggtgcg cgtgcggcgt cgcggcggcg gcccttctcg ccgatgtcgt
     7861 ttcgaaccgc ttctacgcgc gcgatgggac acagcgctcc ttcgggaccg gtctcgcgcc
     7921 ggccgtgttt ccggagatca ccgccggact ggtcgagcgc ctgcggctgg aaggggaggc
     7981 gttccacgat ctcgcggacg ggggctacct cgcctggcgg tggtggccgg accgccgcgt
     8041 ctacatcgac ggccgtctgg aggtgcaccc cgagtcgctg ttcgcgatct ggctgcaggc
     8101 gctctccgat ccggtccgct tcgaggccga agcgcgcgcc cgcggcatcg gcctcgtggt
     8161 ctggagccac cgcagcgccg tcgacgccgc gccgctcctc cgacacctgg cgacctcccc
     8221 gtcctggagg ctcgtgcacc tggatctgga gtcggcactg tttctgcgcc gcgaggcagg
     8281 cggaggcccc gaggtcgcct cggattccga cctcgaagcg ccggcgctcc gcctgctggc
     8341 ggaggcggag gcagcggccg cgcgtgcgcg cgaccaggat ccggcccccg gctggctccg
     8401 ccgcattctg ccgcgcctcg acattccgac aggggagacc ggcgccgcac tgttcctcgc
     8461 gctcaccggc cggcccggaa gcgccgtggc gcttttcgag gatgcctgcc ggcgcgcgcc
     8521 gtggagcgcg ccgctgcgct acgacctcgg cctggcgcgc gcgcaggcgg gcgacgacg
//
LOCUS       DASRIQ010000449         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2256734, whole genome shotgun sequence.
ACCESSION   DASRIQ010000449 DASRIQ010000000
VERSION     DASRIQ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256734"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VFD06_10965"
     CDS             complement(<1..1389)
                     /locus_tag="VFD06_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN04090.1"
                     /translation="MRIKMILPALTEALSPYFRPIKYSLFPPLGLATLAGYCGVDDEI
                     AIQDEHVETLSLEDEPDLVVIQVYITSARRSYELADHYRRRGVHVCLGGLHVTSLPEE
                     ARAHADTIFIGPGEDTWPRFLRDFRAGRPGAEYRSTVRTLEGVPPIRRDLIKRHLYFV
                     PNSIVVSRGCPHVCDFCYKEAFFEGGRGFYTQGVDDALAEVARLPGRHLYFLDDHLFG
                     NVPFASALFDGMRGMGRLWQAAATVESVLRPGLLERAVDCGLRSLFVGFETLNRVALR
                     EQRKFQNLRRDYGTAVRRLHDCGVMVNGSFVFGMDGEDADVFERTVEWAIEQGIETAT
                     FHILTPYPGTALYRRMQASGRLLHSNWDRYDTRHVVYRPEGIDPSRLESGYWWAYREF
                     YRWGSIFRGASHKPRLSGGLRHVAYAAGWKKFEALWNVVIRTHRLIHTLPVLETVLSE
                     FGRRAPSGSGVVT"
     gene            complement(1386..1874)
                     /locus_tag="VFD06_10970"
     CDS             complement(1386..1874)
                     /locus_tag="VFD06_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZN04091.1"
                     /translation="MEAIDLSVQAPAIYHDRSEIRTEDRMQFIDITGWVARRLRLSGI
                     DRGIALVRVLHTTAAIVVNENEPLLLDDLKAMLERLAPEGETYAHDDFLRRRVNVEPG
                     ERRNGHAHARALLLGESKQIGVLEGSLELGRWQSIFLVELDGPRRRTVSVTVMGSRRG
                     QP"
     gene            2038..2946
                     /locus_tag="VFD06_10975"
     CDS             2038..2946
                     /locus_tag="VFD06_10975"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZN04092.1"
                     /translation="MSDRSRDDTHSLPASGRRGTDQAPPTTAVRIDVAGLSDKGKVRD
                     ENQDHFLVVRAGRYAETLMTSLPPGAIPERSEDRGLLMMVADGMGGQAAGELASRTVL
                     VTLTHLILNHPEWAMKVDERSAAGIEDRAVARYQALDAALADRMQEDPSLKGMGTTLT
                     VAYSLGLDVFIAHAGDSRAYLLHEGSIRQLTHDHTYVQRLVDAGMLSPEAAAAHQLRN
                     ALTNVLGGGKRSVDVDLQRLRVAAGDRLLLCSDGLTGTMKDEEIADLLGRSASAEDSC
                     RRLVDLALERGAPDNVTVIVARYELS"
BASE COUNT          544 a         1080 c         1094 g          472 t
ORIGIN      
        1 cgtcaccacg ccggacccgg agggagcgcg tcttccgaac tccgagagga ccgtctccag
       61 cacggggagg gtgtggatca gccggtgcgt ccggatgacg acgttccaca gggcttcgaa
      121 cttcttccat ccggcggcgt aggcgacgtg ccgcagcccg ccgctcaggc ggggcttgtg
      181 cgacgcgccg cggaagatcg atccccagcg atagaattcc cggtacgccc accagtaccc
      241 cgactccagc cgggacgggt cgattccctc gggtcgatag acgacgtggc gcgtgtcgta
      301 ccggtcccag ttgctgtgaa gcaggcgtcc ggaggcctgc atgcggcgat agagagcggt
      361 gccggggtag ggcgtcagga tgtggaacgt tgccgtctcg atcccctgct cgatcgccca
      421 ctccaccgtg cgctcgaaga cgtcggcgtc ttcgccgtcc atgccgaaga cgaagctgcc
      481 attcaccatc acgccgcaat cgtgcagcct tcggaccgcc gtgccgtagt cgcgtcgcag
      541 gttctggaac ttgcgctgct cgcgcaacgc cacccgattg agggtctcga atccgacgaa
      601 caggctgcgg agcccgcaat cgaccgccct ttcgagcaag ccagggcgca ggaccgattc
      661 caccgtggcg gccgcctgcc acagccgccc catgccgcgc atcccgtcga agagggccga
      721 ggcgaacggc acgttgccga acagatgatc gtcgaggaaa tagaggtgcc gccccggcag
      781 gcgggcgacc tccgccaggg cgtcgtcgac tccctgcgtg tagaagcccc tgcctccctc
      841 gaagaacgcc tccttgtaac agaagtcgca cacatggggg cagccgcggg agacgacgat
      901 cgagttgggg acgaagtaga gatgccgctt gatcaggtcg cgtcggatcg gcggcactcc
      961 ctccagcgtc cgcaccgtcg agcggtattc ggcgccgggc ctccccgccc ggaaatcgcg
     1021 aaggaatcgg ggccaggtgt cctcgccggg tcctatgaag atggtatcgg cgtgggcgcg
     1081 cgcctcctcc ggcaacgagg tgacgtgcag acctcccagg cagacatgca ccccgcgacg
     1141 ccgatagtgg tccgccagct cataggagcg ccgggcggag gtgatgtaga cctggatcac
     1201 gaccaggtcg ggctcatcct ccaaagagag cgtctccacg tgctcgtctt gaatcgcgat
     1261 ctcgtcgtcc acgccgcaat acccggcgag cgtggcgagc ccgagaggcg ggaacaggga
     1321 gtacttgatc ggccggaagt aggggctcaa cgcctccgtc agcgccggga ggatcatctt
     1381 gattctcatg gttgaccgcg ccgggacccc atcacggtca cggaaaccgt gcggcggcgg
     1441 gggccgtcga gctcgaccag gaagatcgac tgccagcggc ccagttccag gctcccttcg
     1501 agcacgccga tctgcttcga ttctcccagc aggagagcgc gcgcgtgggc gtgtccgttg
     1561 cggcgctctc cgggctcgac gttgacgcga cgccgcagga agtcgtcgtg ggcgtaggtt
     1621 tcgccttccg gcgcgaggcg ctcgagcatc gccttcaagt cgtcgaggag caggggctcg
     1681 ttctcgttga cgacgatggc ggcggtcgtg tgcaggaccc ggaccagcgc gatcccacga
     1741 tcgatcccgg agaggcgcag gcgccgggcc acccatccgg tgatgtcgat gaactgcatc
     1801 ctgtcctcgg tccggatctc ggaccggtcg tggtagatcg ccggtgcttg aacactgagg
     1861 tcgatggctt ccatggggtc ctcgatcgat gcgccgcgtc agtgcggcaa tggccggggg
     1921 gaaggtcgca ccaggagagg gaaaagacct gagcctcgga tgagcagcgg gtgaggattc
     1981 ggtgaggcgt ggcgtccggg accgggacta taatcccccc acggaaccca accgacgatg
     2041 agcgacaggt ccagggacga cacgcattcg ctgcccgcgt ccggccggcg cggcaccgac
     2101 caggcaccgc cgacgaccgc ggtgcggatc gacgtcgccg gtctctccga caaggggaag
     2161 gtccgggacg agaaccagga tcattttctg gtggtgcgcg ccggacgcta cgccgagacg
     2221 ctgatgacca gcctgcctcc gggagcgatc ccggagcgct ccgaggatcg ggggctcctg
     2281 atgatggtgg ccgacggcat gggcgggcag gcggcgggtg aattggcgag ccgcaccgtc
     2341 ctcgtcaccc tgacccatct gatcctcaac caccccgagt gggccatgaa ggtcgacgag
     2401 aggagcgccg ccggcatcga ggaccgcgcc gtggcccggt accaggcgct cgacgctgcc
     2461 ctcgccgacc gcatgcagga ggacccgagc ctgaagggca tggggacgac cctgacggtc
     2521 gcctacagcc tcggtctcga cgtcttcatc gcgcacgccg gagactcccg ggcgtatctt
     2581 ctgcacgagg ggtccatccg gcagctcacc cacgaccaca cgtacgtgca acgactggtg
     2641 gacgcgggga tgctgagccc cgaggccgcg gccgcacacc agctgcgcaa cgccctcacc
     2701 aacgtcctgg gcggcggaaa gagatccgtg gacgtggatc tccagcggct gcgggtcgcg
     2761 gcgggcgacc ggttgctcct gtgcagcgac ggcctcaccg gcacgatgaa ggacgaggag
     2821 atcgccgacc tcctgggccg gtccgcctcg gccgaagact cctgccgccg tctcgtcgat
     2881 ctcgcgctcg agcgcggcgc ccccgacaac gtcaccgtga tcgtggcgcg gtacgagctc
     2941 tcctgaggcc tccggcgtcc gggagtagaa gcccggccgg tgcagatcgg ctgaagtaac
     3001 ggcccgactc ccggcgcctc gccgccggtc atggtgcggc acgcgccccg cgcagcatcc
     3061 ttcgcacctg cgcagccagc tgcgcgcgcg tcacccacgc ggcctgcaaa tccctcggat
     3121 cgagagcgac tttccagagt cagaccagcg gcacgccgtc tgcaggacgg acgcgcgcgg
     3181 aggagacgac
//
LOCUS       DASRIQ010000450         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2506716, whole genome shotgun sequence.
ACCESSION   DASRIQ010000450 DASRIQ010000000
VERSION     DASRIQ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506716"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFD06_10980"
     CDS             complement(<1..386)
                     /locus_tag="VFD06_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZN04093.1"
                     /translation="MSDAATRGAIEAVFRIEAPRLIAALARFTRDVGRAEDLAQDALV
                     AALEEWPRQGVPENPAAWLMATAKHRAIDLFRRDTRLARIHEDLAHETGDAGSAGAGT
                     DREGDDVGDDLLRLMFVACHPVLPPES"
     gene            complement(383..811)
                     /locus_tag="VFD06_10985"
     CDS             complement(383..811)
                     /locus_tag="VFD06_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN04094.1"
                     /translation="MRFMMLMIPKGYEKAAPGAMPDARAVAAMMKYNEALQKAGVLLA
                     LDGLHPPSMGARVTFPGGRPAVKDGPFAEAKEVVGGYWMIQVGSREEAIEWAKRCPAG
                     ENEMIEVRQVQEMSDFPEDVRQAASGFEEMQKGHGPGGKA"
     gene            945..1160
                     /locus_tag="VFD06_10990"
     CDS             945..1160
                     /locus_tag="VFD06_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:981..983,aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HZN04095.1"
                     /translation="MPNSVRIFGKDTUPYTMAAREGFAAKNYEVQYHNVQKDKALLEE
                     MLALTKGRRSVPVILEGGKVTIGYGGT"
     gene            1211..>2130
                     /locus_tag="VFD06_10995"
     CDS             1211..>2130
                     /locus_tag="VFD06_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04096.1"
                     /translation="MTASDRARFLVQFGESLERIDDSIKLHKTALAQAARYFSAARGA
                     VVLRNPVSGALTTYGWGEDGGWDDRTLHAFIDLERPALPATDILAPLLIGERAAGLIA
                     LRREGGFPPGAGRFLARLGRKLGHEAGLRDERRVQRAVDQIKERTLRDLRPQDLFYQI
                     LHGLRTLTRYDHSSALLIAEDDGARLVLHAEQVAWTKGKSRRIGKRVRVPQELRAALR
                     RPGAAPVVWSEPPRPRRRKGSPPASPQAPAAPAAPAAGEGLAHLLQWGGRGREPQEET
                     ILFATLHHDGTLLGVLKIAAQRPRAFGPPEA"
BASE COUNT          302 a          787 c          731 g          310 t
ORIGIN      
        1 gactcgggcg gcaggaccgg atggcaggcg acgaacatga ggcgcaggag gtcgtcgccg
       61 acgtcatccc cctcgcgatc cgtcccggca ccggcgctcc ccgcgtcgcc ggtctcgtgc
      121 gcgagatcct cgtggatgcg cgccagccgc gtgtcgcgtc ggaagagatc gatggcgcgg
      181 tgcttcgccg tcgccatcag ccacgccgcg gggttctccg gcaccccctg acgcggccac
      241 tcctcgagcg cggcgacgag ggcgtcctgc gcgagatcct cggcccgccc gacgtcccgc
      301 gtgaaccggg ccagcgcggc gatgagccgc ggcgcctcga tgcggaagac cgcctcgatg
      361 gcgccgcggg tggccgcgtc gctcacgcct tgccgccggg cccgtggcct ttctgcatct
      421 cctcgaaacc ggaggcggcc tggcggacgt cctccgggaa gtcggacatc tcctgcacct
      481 ggcgcacctc gatcatctcg ttctcgccgg cggggcagcg cttcgcccac tcgatcgcct
      541 cttcgcgcga cccgacctgg atcatccagt agccgccgac gacctccttg gcctccgcga
      601 acggcccgtc cttcacggcc ggccggccgc ccgggaaggt gacccgcgcc cccatggacg
      661 gcgggtgcag gccgtcgagc gccaggagca ctccggcctt ctgcaaggcc tcgttgtact
      721 tcatcatcgc cgcgaccgcc ctggcgtccg gcatcgctcc cggcgccgcc ttctcgtacc
      781 ccttgggaat catcagcatc atgaatcgca tcgtcgttcc ctccctttca cttcgacgtc
      841 gaacgaaccg gcgtcggatc gacacccggc agccggtttt ttcgagcgcg ttgatcggct
      901 tctcgtcgcg atgctacgat gcggcgccgg tcggaggaaa atcgatgccg aactcggtgc
      961 ggatcttcgg gaaggacacc tgaccctaca cgatggcggc ccgggagggc ttcgccgcga
     1021 agaattacga agtccagtac cacaacgtcc agaaagacaa agccctgctc gaggagatgc
     1081 tcgccctgac caaggggcgg cgcagcgtcc cggtgatcct cgaggggggg aaggtcacca
     1141 tcggctacgg cgggacctga ggcgtctgag ccttcggccg gcggccgaaa ccagaagccc
     1201 ggagcccgcc atgactgcga gcgaccgcgc ccgcttcctc gtccagttcg gggagtcgct
     1261 cgagcgcatc gacgattcga tcaagctcca caagaccgcc ctcgcccagg cggcgcggta
     1321 cttttccgcc gcgcgggggg ccgtcgtgtt gaggaacccg gtttcggggg cgctcacgac
     1381 ctacggctgg ggcgaggacg gtggctggga cgacaggacg ctccacgcct tcatcgatct
     1441 ggaacggccg gcgctgcccg cgaccgacat cctcgccccg ctcctgatcg gggagcgggc
     1501 cgccgggctc atcgcgctgc gccgcgaagg ggggttccct cccggcgccg gacggttcct
     1561 ggcgcggctc ggccgcaagc tggggcacga ggcgggtctc cgcgatgagc gccgcgtgca
     1621 gcgggccgtc gatcagatca aggagcggac gcttcgggat ttgcgcccgc aggacctctt
     1681 ctaccagatc ctccacgggc tccggacgct gacccgctac gaccattcct cggcgctgct
     1741 catcgccgag gatgacggcg cgcggctcgt gctccacgcc gagcaggtcg cgtggaccaa
     1801 ggggaagagc cggcgcatcg gcaagcgcgt ccgggtcccc caggagctgc gcgccgcgct
     1861 gcggcgcccg ggggcggcgc cggtcgtctg gtcggagccg ccgcgccccc ggcgcaggaa
     1921 gggaagcccg cccgcttcac ctcaggcgcc cgcagcgccg gctgcccccg ctgccggcga
     1981 agggctggcg cacctgctgc agtggggcgg caggggacgc gagcctcagg aagagaccat
     2041 cctcttcgcg acgctgcacc acgatggaac gctcctcggc gtgctgaaga tcgccgcgca
     2101 gcgcccgcgc gccttcggtc ctccggaggc
//
LOCUS       DASRIQ010000451         4786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_954775, whole genome shotgun sequence.
ACCESSION   DASRIQ010000451 DASRIQ010000000
VERSION     DASRIQ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954775"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFD06_11000"
     CDS             complement(<1..478)
                     /locus_tag="VFD06_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04097.1"
                     /translation="MRRAAFLFPLVLFFGAGVAAAAEPPQALFDRAGAAYREGKFDEA
                     ARGYEAILAQGVHDPRVHYNLANAYFKQGRLGPAILEYERSLRLDPSDDEARDNLALA
                     RGLLRDKVTEPELQYPVRVLKETIETLPSGVLTGGFLVFWFGAWGAMGLVPVTASWV"
     gene            complement(475..2331)
                     /locus_tag="VFD06_11005"
     CDS             complement(475..2331)
                     /locus_tag="VFD06_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HZN04098.1"
                     /translation="MRFPRRAGLLPAILLILLPSAAAAQDVRVRAQVDRSEMSEGEDI
                     RLIVEIAGPGLDRVGPPDVHDLGDFTISGGPSVSSRFQWVNGVSTSSKTYTYALTPTR
                     TGKLTIPSLAILAQGRTYRTNPVDVEVHAQGTIRNPSAPPPPGPPAPGPQGGGGGGGG
                     RRPGAAAVALRVRADLDRREVYVGQQVTLKVVLDTQSEILSHLPAESPSFPGFWAEEI
                     ALPERTEVRRVSIEGEPWYEITLMKRALFPTTAGTLSVPPIAWQVQVRRRSADPFESF
                     FFTPTEVVTRRSDPVDLKVLPLPPAGRPAGFSGAVGSFTLSVTADRNESRVNDAVGLK
                     VRVQGEGSLGSATPPGLGDLVDFKTFDPKITSNTTLQTDRLRSEKIWDYVVIPLAPGS
                     QTIPPVAFSYFDPEARAYRTVSSKALPIQVARGEAIDSGSGIAGLPQSDIRLLRRDIH
                     YLKAAPQGLKARSAPFHQTPWFVALCLAPMLADVGILAWARTRDLSPQASRSRRERRA
                     RAAARRRLAHARRLMKPASAHPFYASVAQALTGYVGDKFHAAGAGLTHERIEALLAER
                     GVPDDRRAAFHRCLEACDFARFAPSSSDDAAMRSALASAEEAIAGVERSLDR"
     gene            complement(2328..4016)
                     /locus_tag="VFD06_11010"
     CDS             complement(2328..4016)
                     /locus_tag="VFD06_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN04099.1"
                     /translation="MRFGASFYLWFLFAVPVIAGLLALDAHRRRRLLERLGDRPLVAR
                     LSQSLSVEKRIIKGLLVVVAAAFLVLALARPQWGMKTETVKRRGVDVMIAVDTSLSML
                     SEDMKPNRLDQARSAIRGMFDLLQGDRVGLVAFAGTAYVACPLTLDYGAAAMFVDILD
                     TDLLPVRGTAIAPAIRAALDAFPKEEGRHKVIVLVTDGEDHEGDIEAAVNEAVERGIV
                     IHTVGVGAPGGVPIPIRNARGDVTGYKEDRERRKVTSRLDEGTLQSIAAATHGKYFRS
                     TPEGIELKGVYDEIAAMDPKNMTSRTVTRFEERYIIPLGAALLLLGLETALGDRRRRA
                     RAAAAAAMVLALLLPSRAIADPAAKNNQGNRQYESKRYDEALKSYTDAQAERPAAPEI
                     HYNIGNVLLRKGDVDKAIEEYLRAQSTGPKSLTEAAHFNRGNALMMKGELQPAVDAYV
                     QALKIDPRDPDAKRNLELALRLLEQQQQQQQQQQQQQQQDQPQDQKKPQQPPPQPDKE
                     KQEDSQQPQPRPGEMSPEEAKQILEALRESEKESLKKHAQASAPPNTRDPEKDW"
     gene            complement(4042..>4786)
                     /locus_tag="VFD06_11015"
     CDS             complement(4042..>4786)
                     /locus_tag="VFD06_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN04100.1"
                     /translation="LTEGIDILLVLDVSGSMRTEDFRPKNRLVVAKDVVSQFVRGRRN
                     DVIGLVAFAAGAYTRCPLTLDYEVLLEQLARTDFATREEDGTAIGLGLATGVARLEKA
                     SGKSKVIVLLTDGRNNRGQIDPLTASRLAASLGVKVYTIGVGKEGEAPYPIDDPLMGK
                     RYIMVSADIDEDSLKQIAEETGGQYFRATDAGSLKWIFERIDALEKTEIRVRGYTRKT
                     ERFAFFLYPGAVCVALELLLGATLLRRLP"
BASE COUNT          645 a         1693 c         1701 g          747 t
ORIGIN      
        1 gcacccacga ggcggtcacc gggacgaggc ccatggcccc ccacgcgccg aaccagaaga
       61 ccaggaagcc gccggtcagg accccggacg gcagcgtctc gattgtctcc ttgaggaccc
      121 gcaccgggta ctgcagctcc ggctccgtca ccttgtcgcg caggaggccg cgcgccagcg
      181 ccaggttgtc gcgcgcttcg tcgtccgacg ggtcgagccg cagggatcgc tcgtactcca
      241 ggatcgccgg acccaggcga ccctgcttga agtaagcgtt ggcgaggttg tagtggacgc
      301 gcgggtcgtg caccccctgc gccaggatcg cctcgtatcc tcgtgccgcc tcgtcgaact
      361 ttccttcccg gtaggcggcc ccggcgcgat cgaagagggc ctgtggcggc tcggccgccg
      421 cggcgacgcc cgcgccgaaa aaaaggacca gcgggaacag gaaggcggca cgtctcaccg
      481 atcgaggctc cgctcgacgc cggcgatcgc ctcctccgcg gaggcgagcg cggagcgcat
      541 cgccgcgtcg tcggacgacg acggggcgaa acgcgcgaag tcgcaggcct cgaggcagcg
      601 gtggaacgcc gcccgccggt cgtccgggac gccgcgctcg gcgaggagcg cctcgatccg
      661 ctcgtgcgtc agcccggccc ctgcggcgtg gaacttgtcg ccgacgtagc cggtgagcgc
      721 ctgcgcgacc gacgcgtaga acgggtgggc gctcgccggc ttcatgagac gccgcgcgtg
      781 cgccaggcgc cgccgcgccg cggcccgggc acggcgctcg cggcgggagc gggaggcctg
      841 cggggagagg tcccgggtcc tggcccaggc caggatgccg acatcggcga gcatcggagc
      901 caggcacagg gcgacgaacc agggggtctg gtggaacggc gccgaccgcg ccttgagccc
      961 ctgcggggcc gccttgaggt agtggatgtc gcgccgcagc aggcggatgt cgctctgcgg
     1021 cagcccggcg atccccgacc cggagtcgat cgcctcgccg cgcgccacct ggatcggcag
     1081 cgccttgctc gacaccgtcc tgtaggcgcg cgcctcgggg tcgaagtacg agaaggcgac
     1141 cggcgggatc gtctggcttc ccggcgcgag cgggatgacg acgtagtccc agatcttctc
     1201 cgatcgcagc cggtccgtct gcagcgtcgt gttggaggtg atctttggat cgaaggtctt
     1261 gaagtccacg aggtcgccga gcccgggcgg cgtggcggac ccgaggctcc cttccccctg
     1321 gactctcact ttgagcccga cggcgtcgtt gacgcgcgat tcgttccggt cggccgtgac
     1381 cgacagcgtg aacgatccga ccgctcccga gaacccggcg ggccgccccg ccggcggcag
     1441 cggcagcacc ttgagatcca ccgggtcgct gcgtcgcgtc accacctcgg tcggggtgaa
     1501 gaagaacgac tcgaacgggt cggcgctgcg cctccgcacc tgcacctgcc aggcgatcgg
     1561 cggcaccgag agggtcccgg cggtggtcgg aaagagcgcc cgcttcataa gcgtgatctc
     1621 gtaccagggc tccccctcga tcgacacgcg ccgcacctcg gtgcgctccg gcagggcgat
     1681 ctcctccgcc cagaagccgg ggaacgaggg gctctccgca ggaaggtggc tgaggatctc
     1741 cgactgggtg tcgagcacca ccttgagagt gacctgctgc ccgacgtaca cctcgcggcg
     1801 gtcgaggtcg gcgcggaccc gcagcgccac cgccgccgcg ccgggacggc ggcctccgcc
     1861 tccgcctccc cctccctggg gccccggcgc gggcggcccc gggggcggcg gcgcggaggg
     1921 gttccggatc gtcccctgcg cgtgcacctc cacgtcgacc ggattggtgc gataggtgcg
     1981 cccctgcgcc aggatcgcga gcgacggaat ggtcagcttg ccggtgcggg tcggagtcag
     2041 ggcgtaggtg taggtcttgc tcgaggtgct gacgccgttg acccactgga aacggctcga
     2101 cacggacggg ccgccggaga tcgtgaaatc gccgaggtcg tgcacgtcgg gaggacccac
     2161 ccgatcgagc cccggcccgg cgatctcgac gatgaggcgg atgtcctccc cctccgacat
     2221 ctccgatcga tcgacctgcg cccgcacccg gacgtcctgg gccgcggccg cggacggcag
     2281 gaggatcagg aggatcgccg gcaggagccc ggcgcgacgc ggaaacctca ccagtccttc
     2341 tccgggtcgc gggtgtttgg cggcgccgac gcctgggcgt gtttcttgag gctctccttc
     2401 tccgattcgc gcagcgcctc gaggatctgt ttcgcctcct ccgggctcat ctcgccgggg
     2461 cgcggctgcg gctgctgcga gtcctcctgc ttctccttgt ccggttgggg cggaggctgc
     2521 tgcggtttct tctgatcctg gggctggtcc tgctgttgct gctgctgttg ttgttgttgc
     2581 tgttgctgct gttgctcgag caggcgcagc gccagctcga ggttccgctt ggcgtcgggg
     2641 tcgcgggggt cgatcttcag cgcctggacg taggcgtcga ccgccggctg cagctccccc
     2701 ttcatcatca gggcgttgcc gcggttgaag tgggccgcct ccgtcaggct cttgggcccg
     2761 gtgctctgtg cgcggaggta ctcctcgatc gccttgtcca cgtccccctt gcgcaggagg
     2821 acgttgccga tgttgtagtg gatctccggc gcggcggggc gctctgcctg ggcgtcggtg
     2881 tagctcttga gcgcctcgtc gtagcgcttc gactcgtact gtcggttccc ctggttgttc
     2941 ttggcggccg gatcggcgat cgcccgggag gggaggagga gcgcgaggac catcgccgcc
     3001 gcggcggcgg cgcgggcgcg gcggcggcgg tccccgagcg ccgtctccag ccccaggagc
     3061 agcagggcgg cgccgagcgg gatgatgtag cgctcctcga aacgggtcac ggtgcgcgag
     3121 gtcatgttct tcgggtccat cgcggcgatc tcgtcgtaca cacccttgag ctcgatcccc
     3181 tcgggggtgg agcggaagta cttgccgtgc gtcgccgccg cgatcgactg cagcgtccct
     3241 tcgtccagcc ggctggtcac cttgcggcgc tcgcgatcct ctttataccc cgtcacgtcg
     3301 ccgcgcgcgt tgcgaatcgg aatcgggacc ccgccggggg cgccgacccc gaccgtgtgg
     3361 atgacgatgc cgcgctccac cgcctcgttc accgccgcct cgatgtcccc ttcgtggtcc
     3421 tcgccgtcgg tcaccagcac gatcaccttg tgccgcccct cctccttcgg gaaggcgtcc
     3481 agcgcggccc ggatcgccgg cgcgatggcg gtgccgcgga ccggcaggag gtcggtgtcg
     3541 aggatgtcca cgaacatcgc cgccgccccg tagtccaggg tcaacggaca ggcgacatag
     3601 gcggttccgg cgaaggcgac gaggccgacc cggtccccct gcaggaggtc gaacatcccc
     3661 cggatcgccg agcgggcctg gtccagccgg ttcggcttca tgtcctccga cagcatgctg
     3721 agcgaggtgt ccaccgcgat catcacgtcg acgccgcgcc gcttgaccgt ctcggtcttc
     3781 atcccccact gcggccgcgc cagggccagg acgaggaacg ccgccgccac caccacgagg
     3841 agccccttga tgatccgctt ctcgacgctc aggctctggg acagccgcgc gaccagcggg
     3901 cggtcgccga gccgctccag gaggcgccgg cggcggtggg cgtcgagcgc cagcaggccg
     3961 gcgatcaccg gcaccgcgaa caggaaccac aggtagaacg aggccccgaa gcgcatcagc
     4021 gatcctccct cggcccgccg gtcacgggag ccgcctcagg agggtggcgc cgagcaggag
     4081 ctccagcgcc acgcagaccg cgcccggata caggaagaag gcgaagcgct ccgtcttgcg
     4141 ggtatagccg cgcacccgga tctcggtctt ctccagggcg tcgatgcgct cgaagatcca
     4201 cttcagcgac ccggcgtcgg tggcgcggaa gtactgcccg ccggtctcct cggcgatctg
     4261 cttcaggctg tcctcgtcga tgtcggccga caccatgatg tagcgcttcc ccatgagcgg
     4321 gtcgtcaatc gggtaggggg cctccccctc cttgccgacg ccgatggtgt agaccttgac
     4381 gccgagcgac gccgccaggc gtgacgcggt caggggatcg atctgcccgc gattgttgcg
     4441 cccgtcggtc aggagcacga tcaccttgct tttgccgctc gccttctcca ggcgcgccac
     4501 gccggtggcc agccccagcc cgatggcggt gccgtcctct tcgcgggtgg cgaagtcggt
     4561 gcgcgccagc tgctcgagca ggacctcgta gtcgagcgtc agggggcagc gggtgtacgc
     4621 gccggcggcg aaggcgacca gcccgatgac gtcgttgcgc cgcccgcgca cgaactggct
     4681 gacgacgtcc ttggcgacca ccagccggtt cttcgggcgg aagtcctcgg tccgcatgct
     4741 gccggagacg tcgagcacca gcaggatgtc gatcccctcg gtgagc
//
LOCUS       DASRIQ010000452        10940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_715778, whole genome shotgun sequence.
ACCESSION   DASRIQ010000452 DASRIQ010000000
VERSION     DASRIQ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715778"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFD06_11020"
     CDS             <1..348
                     /locus_tag="VFD06_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04101.1"
                     /translation="ATVAATDVANLRGFDPSATWFDWDPMPAGSGTVYDLVRGDLANL
                     SRNAGAVDLGPLTCVENDSPDENSASSPDTETPAPSTAFFYLVRFQVGPVIGPWGFGS
                     EGEKRAGTGGCPP"
     gene            complement(408..629)
                     /locus_tag="VFD06_11025"
     CDS             complement(408..629)
                     /locus_tag="VFD06_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04102.1"
                     /translation="MSKPETFAVNDRVNHAEHGLGKIVEVNSRITTISFDTAGVRKFL
                     NSLMRLERSDSPNPPKPSRAGKKKAAATA"
     gene            1300..3507
                     /locus_tag="VFD06_11030"
     CDS             1300..3507
                     /locus_tag="VFD06_11030"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN04103.1"
                     /translation="MLSRQGSAGTTGLVVKDPSTGRYFRFGEIEAFLLQSLDGTVPLE
                     TIRSRVEERFEAPLSRTALEGFVGRLRSLGLIEERSKVPTGPPRGRLRGDPLYLRFKV
                     FDPDRLLDWMAGRLGFLFTTPFVVLSAIGIAAAIAVSIANQAEIAQAVRGLYNLRSLL
                     TAWVTVYVVMVVHEFAHGLTCKRFGGSVREIGFFLIFFQPAFYCNVSDAWLFPKKSHR
                     IWVTFAGAYAEIFLWALAILVWRVTEPGTVTSQTALVVMATSAIKTLINLNPLIKLDG
                     YYLLSDALEIPNLRQKAMAYLRGWIGRLTGGRTPNGPPTPRREARIFLVYGLVAGTYS
                     VWLLGYVGLWFGGLLVARYQGWGAVLFAILFLAVFRAPISRSLDSLRAMSRTGQALRA
                     SLMRGMKIFAVVTPALAVLWLGKMELKVAGDFNVLPAHNADVRSDVEGIIQEIRVKEG
                     DHVERGDVISVLSDRDYRAEIGQVGAQIEESQARLQMLKEGPRAEEVSLALTSIEKAQ
                     ERLRYAREKRQMLNTLFAQNLVSQKEFAEAEEDVSVRQKELEEAEGRLKMLRAGSRPG
                     EIESVAAETRRWEAHRRYLEDKLMRISIVSPISGVITTPKLEQRVGELVHQGDLIAKV
                     HDLSTVTAEIAVSEYEISDVQVGQKVELRARSYPGRSFHGTVTSIAPTFSRGLEPSAG
                     KSIVVTTRLDNPSLLLKPEMTGTAKIHCGQTRVLSLLTRRLSHTLRLEFWSWW"
     gene            4226..7177
                     /locus_tag="VFD06_11035"
     CDS             4226..7177
                     /locus_tag="VFD06_11035"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN04104.1"
                     /translation="MEDLDTDADETHRLEPEDGPTKEIGHRYRILGLLGKGGMGSVYL
                     ARDRELDRDVALKVIRPHLAGEPGVLERFKREILLSSRITHRNVLRVHDLGQSGHLHF
                     LTMEYVPGQDLATLLKQEGRLALDRSLKIFRQICEGLAAAHTGQVLHRDLKPQNILVD
                     RDDHVHISDFGLAKSLEGGGMSGTGALIGTPHYMSPEQVKGETVDQRADIYALGVILY
                     EILTGSLPFKGGSGWEVALQRLQGPPVPATELNPAIPVYLKTILEHCMSADKSRRYGS
                     VAEILVDLDNARASRALPVHASLRRPAWLAAVAVVVTAGLTWAAWRLLPGVHRPSRGS
                     TESGAPAAPASIVGVVPFENRTGRPDLDWYGAGLARLVMDNLAQSRHVRVVSSETVNA
                     LKGKPEGLTQAARAGGIGFLLSGEIVTAPGGLTVAARLTNTGSGQQAAARRSDGLTEQ
                     TLIGAADEIAVTARRGLGLPPSEGVDVFGADFLARNPAAYADYMKGLEAWTRYRYPAA
                     EAAFREALRIAPDYTMARYRLAQVLAEVGKSDEALTEIRRALSEAPRLPDREGRYIRA
                     SEAYLSRNYDEALKHYREQVERYPYDLEARDLLAVVLKDVEKYDEAAAQLRINAGLEP
                     DRPMTWSMLGDVYLASGDLNQAVLALRRFIELEPSNANGHHLLADAYRTEAELDLAAD
                     EYARALQIDPVFHYSTVSLAEVNVLRGRSDEARRSLQALVSGETVLPRHRIDAAFHLA
                     SIERSGGRFREAAHVLERVGTLLREENVREAMALSTRGLCAVEVGDLRSARPLIDQAI
                     RRSPGVPTRYLFARGLLELREKNTAAVRATAAEILKGSLPAGNPDRTEEKAAAYLTGL
                     AWLVEGQASRAVNDLSRAVTLSGYEYAIYRVVLGRAYLAAGRLPEALAAARQSLAPPG
                     TAEIRLDLELDRARAALLLAAIQARMGKGREAATGARAFLERWAAADPMLPDLAEARS
                     LTSPDPSAAPS"
     gene            complement(7158..8486)
                     /locus_tag="VFD06_11040"
     CDS             complement(7158..8486)
                     /locus_tag="VFD06_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04105.1"
                     /translation="MLKARPVPRRRPMVLGLLSVILAAVGSMGGVIDSSHKPVHPISL
                     QFVGAKRDAVTRQVASGWPITLRLSSNGELGIGRPSLPPFFPRIKEVGYITFADPDNC
                     FSFNAQSTCPVRDETYLEFTPDLDRPGVANPHGNWDRLIGLTDFGGGGSPQVFTFNFQ
                     TLMNDRLASYGPIVGGPEPDLLESRCVGPHVYAGIACTSDGECGPNGTCRLIPVNADG
                     YGLGADDDLPGLVLLSDAGVGVVVDQNFNRPRVPQARNLAGLMSSIAYELNDASARTT
                     VVAHLNVPEGQVYTFPLSAGGDFPARAPRGLFTPVVLVDRCLGDTTTCSHRFDHIFRF
                     DGGPQVTMTDGDMLERLNTQVVTVRAFVVNGTAPSILTDRDHDGVVNSKDAALAGYVL
                     LSGEETIRMVGWYDDELEVTPMLFDFDGNGDARGQLVAPGGAGQITKVPR"
     gene            complement(8497..8934)
                     /locus_tag="VFD06_11045"
     CDS             complement(8497..8934)
                     /locus_tag="VFD06_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04106.1"
                     /translation="MRILTGLIASFLAAGLALPARADAYLACDLSGDVGGAPLSKAVL
                     EVMSGALSGPAVFLFASTTGTVSGWSPAVSQNTAQTAFTSTDSANRVEQLRTGSAGLP
                     KLDSTTVLDDGARDVIAGNQDLDWFFASDDDQVIDRGATEQLN"
     gene            complement(9111..>10940)
                     /locus_tag="VFD06_11050"
     CDS             complement(9111..>10940)
                     /locus_tag="VFD06_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HZN04107.1"
                     /translation="DPRRENRGANFLRAFGRLRAGVTPEQAGAEMATIAARLRERYPD
                     ENAGNLAPRVRSMREEVSGPYRSALLLLLGSVAAVLLIACANLAALQVARTAGRRREI
                     AVRSALGATRGRLLRLLLTESMLLALLGGALGALLAAWGLTGLLALSPSDLPRVAEVS
                     LDWRMLLFAFGATILAGLATGLAPAWRASRRGTSSGLIGTERSAGVGPDAARAQGVLV
                     VAEVAISLTLLVGAGLFLKSYARLMRVDPGIRPAGVLSARLGLTAGGYGTVDRLAALD
                     TDLERRLLALPGVRGAALISVLPLTNVLSRADFTIAGNPPATPAETPSAQNRVISPGY
                     FDVMGIPVIAGRPFTAADDARARGVAVVDAALARRYFPAEDPVGAHLSLDDGGGWRDV
                     EVIGVVGPVRLAALEEPPAPTIYVPFTQLPPAALGNFTGRVHLVVSADADPLALEHEV
                     REALAGVDRGISLSGVRTMPEILSAAVALRRFNLMLMAMFAAAAVLLAASGLFALIAG
                     IVATRTKEIGIRMALGARRAHVLRRVLARSARLAAAGLAAGLLGAVALSRAVSGLLFE
                     VRPADPTILLVTTCGLGAVAMLASLLPALRATRIDPARALRAE"
BASE COUNT         1838 a         3662 c         3685 g         1755 t
ORIGIN      
        1 gcgaccgtcg cggccacgga cgtcgcgaac ctgcgcggct tcgacccctc ggccacctgg
       61 ttcgactggg atccgatgcc ggccggaagc ggcaccgtct acgatctcgt ccgcggagac
      121 ctggcgaacc tctcgaggaa cgcgggcgcg gtggatctgg ggccactgac ctgcgtcgag
      181 aacgactccc ccgacgagaa ttccgcctcc agcccggaca ccgagacgcc tgcgccgagc
      241 accgcgtttt tctacctcgt ccggttccag gtgggaccgg tgatcggtcc gtggggcttc
      301 ggaagcgagg gggaaaaacg agccggcaca ggcggctgcc caccctagag gtcgccaggc
      361 ctaaatgaag aagatgtggc agttaggccc tgccccgcgc cggcggctca ggccgtcgcg
      421 gcggccttct tcttgccggc ccgagagggc ttgggcggat tcggactgtc gcttcgctct
      481 agccgcatca aggagttgag gaacttcctg acgccggcgg tgtcgaagct gatggtcgtg
      541 atccgggaat tcacctcgac gatcttcccc agaccatgct cggcgtggtt gacgcggtca
      601 ttgacagcga acgtctctgg cttgctcaat cgaatcacct catcatcaat ctagattgtg
      661 ggggaagaat aacgcattta ccccctccag cgccaccgga cgcctgtggg gggcctgctc
      721 cgggcccatc cgtcccgcct cgctcaacaa ggaggccccg aggggctaag cagatgcgct
      781 gtcagggctt cctgacgacc ggcaactgag aaactgtcac gaaaaaacaa ctacccggcg
      841 ggcgcgattc gctgtagatt ccgccaacac tccctattgg cagttcgggc gtggggtcgg
      901 ctctctccgg ggggagtgcg agacgctcga gcccatcctc atcgctcgac acgaattccc
      961 aagaggaggt cctcaccatg aagggcagcg aaatccgact tgagatcgag gcgctcgagg
     1021 agagaattgc tcccagcgtc gctccggggc tccagggtta cgaaggccag cctggcaacc
     1081 agggagggta agtcgcccac ggtttggatc cccggggggg ctgcgtagag ggttttcagg
     1141 ctaaccctca ggggtttcgt gcggcaggtg aaggccactg ggttccagtg gccttcttct
     1201 tttttcccga tgcatagcat cgttccgacc ccggcggttt cctcgacccc gacggagccg
     1261 caaggctcgg cggagctgcc gaggctacgc caggacctgg tgctgagccg gcagggctcg
     1321 gcgggaacca ccggcctggt ggtcaaggac ccctccaccg ggcgctattt tcgcttcggc
     1381 gagatcgaag cgttcctcct gcagtccctg gacgggacgg tccccctgga gacgattcgc
     1441 tccagggtcg aggagagatt cgaggcgcct ctctcccgaa ccgccctgga aggtttcgtc
     1501 gggaggctgc gctccctcgg gctgatcgag gagcgctcga aggtgccgac cgggccgccc
     1561 cgcggccggt tgcgcggcga tcctctctac ctcaggttca aggtcttcga cccggatcgg
     1621 cttctcgact ggatggccgg caggctcggc ttcctcttca ccacaccatt cgtggtcctc
     1681 tccgcgatcg gcatcgccgc ggccatcgcc gtcagcatcg ccaaccaggc cgagatcgcg
     1741 caggcagtcc gcggtctgta caaccttcga tcgctcctca cggcctgggt gacggtctac
     1801 gtcgtcatgg tcgtccacga gttcgctcac ggcctgacct gcaagcgctt cggtgggagc
     1861 gttcgcgaaa ttggattctt tctgatcttc ttccagccgg ccttctactg caatgtgagc
     1921 gacgcctggc tctttccaaa gaagtcgcat cggatctggg tcaccttcgc gggagcctac
     1981 gcggagatct tcctgtgggc cctggcgatc ctcgtttggc gcgtgaccga accgggcacc
     2041 gtcaccagcc agacggccct ggtcgtgatg gcgacttcgg cgatcaagac gctcatcaac
     2101 ctgaatccgc tcatcaagct cgatggctat tacctgctga gcgacgccct cgagattccg
     2161 aacctgcgcc agaaggccat ggcctacctg cgcgggtgga tcgggaggct gaccggcggc
     2221 cgaaccccga acggcccccc cacgcctcgc agggaagcgc gcatcttcct cgtctacggg
     2281 ctcgtcgccg ggacctactc ggtctggctt ctgggctacg tcggtctgtg gttcggaggt
     2341 ctcctcgtcg cccggtacca gggctggggg gcggtcctct tcgcaatcct gttccttgcc
     2401 gttttccgcg caccgatcag tcgatcgctt gatagtctta gagcaatgtc ccgaaccggc
     2461 caggccctcc gcgcttcact catgagggga atgaagatct tcgccgtcgt cacgcccgcc
     2521 ctggcggtcc tgtggctggg gaagatggag ctgaaagtcg ccggagactt caacgtcctg
     2581 cccgcacaca acgccgacgt ccggtcggac gtcgagggaa tcatccagga gatccgggtc
     2641 aaggaggggg accacgtcga gaggggagac gtcatttcgg tcctttccga tcgcgactac
     2701 cgtgcggaga tcgggcaggt cggggctcag atcgaggaga gccaggccag gctccagatg
     2761 ctgaaggaag gcccgagagc cgaggaagtc tcccttgccc tgaccagcat agagaaggcg
     2821 caggagcgcc tgcggtacgc ccgcgagaag cgtcagatgc tgaacacgct cttcgcgcag
     2881 aacctggtgt cgcagaagga gtttgccgag gccgaagagg atgtttcagt gcggcagaag
     2941 gagctcgaag aggccgaagg gcggctcaag atgctcaggg caggcagccg tcccggagag
     3001 atcgagtcgg tcgcggcgga gacccgcagg tgggaggctc accgccgcta cctggaggac
     3061 aaattgatgc gaatcagcat cgtcagcccg atctccggag tcatcacgac tccgaagctc
     3121 gagcaacgtg tcggtgagct cgtccaccag ggagatctga tcgccaaggt ccacgatctg
     3181 agcacggtga cggcggagat cgcggtctcg gaatacgaga tctccgatgt gcaggtcggc
     3241 cagaaggtgg agctgcgggc gcggtcctat ccggggcgtt catttcacgg gaccgtcaca
     3301 tcgatcgcgc ccaccttctc gcggggcctg gagccgtccg cagggaagtc gatcgtggtc
     3361 accacgcggc tcgacaaccc ctccctgctg ttgaagcccg agatgaccgg caccgccaag
     3421 atccattgcg gccagacccg agtcctgtcc ctgttgacgc gcaggctctc ccacacgctc
     3481 cgcctcgagt tctggtcctg gtggtgacgc aggcggtcgg ggctcgagtg caggccgggc
     3541 tcgcgtaaga cggcccccgc tcgtgtcgtg gtgtccgggg gccgccctga tcgaggtggt
     3601 gcctttaggt cgcgcgactc agtgatgcat cgtagggacc gagtcggagg ccttcatcgg
     3661 aggactgttc acagtactga cgaatgcctc cttgcccgct ttggtccccc gcacgtaggc
     3721 ttcctgaagc gcggacgcga cccgcgacgc ccggctggcc aggtctcccg tggcctgctt
     3781 gatcttccga cgggtttccc ggccggattg aggtgcggtc agaagggcga cgacagcccc
     3841 tgttgcggca cccgccagga gtaacgtgag ggttcgaacg gtggcgttca tacatcctcc
     3901 tctcttgaat gttgccgcgc tggtcgctgc caggcaacga aacgatgatc gctcgggccc
     3961 gggtcgtcgg ttcaacgacg acgatgggtc gcgagacatt cgtcacggtc ggagggggcc
     4021 cggctggatt gacgatcacg gcaagtcaac tgcactcgca agttagcacc gaatcgcgaa
     4081 cgcgaccacc aaccgttccg aaggacctgt agcgctggcc gtcaatcttg cattcacaat
     4141 ccccctgagg catcctgccc ggacgacccg atgccgctcg tggctgcatc aggccccggc
     4201 ccgaccggag agcgacgggt gacatttgga agatttggat accgacgccg acgaaaccca
     4261 ccggctcgag ccggaagacg gcccgaccaa ggagatcggc caccggtatc gcattctcgg
     4321 gcttctcgga aaagggggga tggggtcggt ctacctcgcc cgcgatcggg aactggaccg
     4381 cgacgtcgcc ctcaaggtca tccgtccgca ccttgccggt gagcccgggg tcctggaacg
     4441 attcaagcgc gagatcctgc tctcgagcag gatcacccac cggaacgtcc tgcgcgtcca
     4501 cgatctcggt cagagcgggc atctgcactt cctcaccatg gagtacgtgc cgggccagga
     4561 cctggcgaca ttgctcaagc aggaaggaag gctggcgctc gatcgctcgc tgaagatctt
     4621 ccgtcagatc tgcgaaggcc tcgccgccgc gcacacgggg caggtcctgc atcgcgatct
     4681 caagccgcag aacatcctgg tcgatcgcga cgatcatgtc cacatctccg acttcgggct
     4741 ggcgaaatct ctggaaggtg gagggatgag cgggaccggc gccctcatcg gcactcctca
     4801 ttacatgtct ccggagcagg tgaagggtga gacggtggat caacgggcgg acatctatgc
     4861 cctgggtgtg atcctctacg agattctcac cggttcgttg cccttcaagg ggggcagcgg
     4921 ctgggaagtg gcgctgcagc ggctccaggg cccgcccgtc cccgcgacgg aattgaaccc
     4981 ggcgattccc gtctatctga agaccattct cgagcactgc atgtcggccg acaaatcccg
     5041 gcgttacggg tccgtcgcgg agatccttgt agacctcgac aacgcgcggg cgtcacgggc
     5101 gttgcccgtc catgcgtccc tccggcgccc ggcctggctc gcggccgtgg cggtcgtcgt
     5161 gacggccggt ttgacctggg cggcctggcg actcctcccg ggagttcatc gcccgtcgcg
     5221 cggttcaacg gaaagtggcg cgcccgcggc cccggcgtcc atcgtcggag tcgtgccgtt
     5281 cgagaatcgc accgggcggc ccgatctgga ctggtacggc gcaggtctcg cccgactggt
     5341 catggacaat ctggcccagt cccgccacgt ccgagtcgta tcgagcgaga ccgtcaacgc
     5401 tctgaaaggc aagccggaag gtttgacgca ggccgcgcgc gcgggtggaa tcgggttcct
     5461 tctcagcggg gagattgtca ccgctccggg agggctgacc gtggcggccc gcctgacgaa
     5521 tacgggcagc ggtcagcaag cggcggcccg caggagcgac ggcctcacgg agcagacttt
     5581 gatcggcgcc gcggacgaga tcgccgtcac cgcccgcagg ggcctcgggc ttcccccttc
     5641 cgaaggggtc gatgtattcg gggccgattt tctggcgcgc aatccggcgg cgtacgccga
     5701 ttacatgaag ggactcgagg cgtggacgcg ctatcgctac cccgcggcgg aggccgcctt
     5761 ccgcgaggcg ctgcgcatcg cgccggatta cacgatggcc cgctaccggc tcgcacaggt
     5821 gctggccgag gtcggcaagt ctgacgaggc gctcacggaa atccgccgtg ccctgtccga
     5881 ggcgccccgg ctccccgacc gcgagggtcg ctatatccgg gcttcggagg cttacctctc
     5941 gcgcaactac gacgaggcgc tgaagcacta ccgggagcag gtcgagcgct atccatacga
     6001 tctcgaggcg cgcgatctcc ttgcggtcgt tctgaaggac gtggagaagt acgacgaagc
     6061 ggcggcgcag ctccggatca acgcgggtct ggagcccgat cgcccgatga cctggagcat
     6121 gctgggagac gtctatctcg cctccggtga cctcaaccag gcggtcctgg ccctgcgccg
     6181 gttcatcgag ctggagccgt ccaacgccaa cgggcaccat ctgctggcgg acgcctatcg
     6241 cacggaggcc gagctcgacc tggccgccga cgagtacgcc agggcgctgc agatcgatcc
     6301 cgtgtttcac tactccacgg taagcctggc ggaggtgaac gtcctgcgcg gacggagcga
     6361 cgaggcccgc cgcagccttc aggcgctcgt ctccggcgag acggtcctgc cgcgacaccg
     6421 gatcgatgcc gccttccatc tcgcgtcgat cgaacgctcc gggggacgct tcagggaggc
     6481 ggcgcacgtt ctggagcgcg tcgggaccct cctgcgggaa gagaacgtgc gcgaggccat
     6541 ggccctgtcg acgcggggcc tctgtgccgt cgaggtcggt gatctgcgct ccgcgcggcc
     6601 cttgatcgac caggcgatca ggaggtcgcc gggggtgccc acgcgctacc tcttcgccag
     6661 ggggctcctg gagctgcggg agaagaacac ggcggcggtc cgcgccaccg ccgccgagat
     6721 cctgaagggg agcctgcccg cgggcaaccc cgatcggacc gaagagaaag cggccgccta
     6781 cctcaccggc ctggcatggc tggtcgaagg acaggcctcc agggcggtca atgatctctc
     6841 tcgcgccgtg accctgtcgg gatacgagta cgcgatctac cgggtcgtac tcggtcgcgc
     6901 ctatctggcg gcgggacgtc ttcccgaagc gctggccgcg gcccgccagt ccctggctcc
     6961 gccgggcacc gccgagatcc gcctggacct cgagctcgat cgggcgcggg ccgcgctgct
     7021 cctcgccgcg atccaggcca gaatggggaa gggcagggag gccgcgaccg gcgcccgggc
     7081 cttcctggag cgctgggccg cggccgatcc gatgcttccc gatctcgcgg aggcccgatc
     7141 cctcacgagt ccggacccta gcgcggcacc ttcgtgatct gtcccgcccc gcccggggct
     7201 acgagctgac ccctggcatc gccgttcccg tcgaagtcga acagcatcgg tgtgacctcg
     7261 agctcgtcgt cgtaccaccc gaccatccgg atggtttctt cgcccgacag caggacgtat
     7321 ccggccagcg cggcgtcctt ggagttgacc acgccatcgt gatcccgatc cgtgaggatc
     7381 gagggggctg tcccattgac cacgaaggcg cgcacggtga cgacctgggt gttcaaacgt
     7441 tcgagcatgt caccgtcggt catggtcacc tgcggcccgc cgtcgaagcg aaaaatgtgg
     7501 tcgaagcggt gcgagcaggt ggtggtgtcg cccaggcagc ggtcgacgag gacgaccggg
     7561 gtgaagagtc cgcggggagc ccgggccggg aaatcgcccc ccgcgctgag cgggaaggtg
     7621 tagacctgtc cctcggggac attcaggtgc gcgaccaccg tggtccgggc gctggcgtcg
     7681 ttgagttcgt aagcgatcga gctcatcagt cccgccagat ttcgcgcctg cggcactctg
     7741 ggacgattga aattctggtc gacgaccacc ccgacgccgg cgtccgacag gagcaccagg
     7801 cccggaagat catcatcggc tcccaggccg tagccatcgg cgttgaccgg aatcaatcgg
     7861 cacgtgccgt tcgggccgca ttcgccgtca gaagtgcacg cgatcccggc atagacatgc
     7921 gggccgacgc accgtgattc cagaaggtcg ggctccgggc cgcccacgat cggcccgtag
     7981 ctcgcgaggc ggtcgttcat gagagtctgg aagttgaacg tgaagacctg cggactgccc
     8041 ccgccgccga aatcggtcag gccgatcagc cgatcccagt tgccgtgcgg gttcgcaacc
     8101 cccggcctgt cgagatccgg cgtgaattcc aggtacgttt cgtcccggac ggggcaggtg
     8161 ctctgtgcgt tgaatgagaa acagttgtcc ggatccgcga aggtgatgta accgacctcc
     8221 ttgatccggg ggaaaaaggg ggggagagac ggcctgccga tccccagctc tccattgctc
     8281 gagagacgca gagtgatggg ccagccgctg gccacctgcc gggtgaccgc atcgcgcttg
     8341 gcgccgacga actgaaggct gatgggatgg acgggtttat gactactatc gatcaccccc
     8401 cccatcgaac ccaccgcggc gagaatgacg gagagcaatc ccaggaccat cgggcgacgt
     8461 cggggaacag gacgagcctt gagcatggcg aactccttag ttgagttgtt ccgtcgcgcc
     8521 ccgatcgatg acttgatcgt cgtccgacgc gaagaaccag tcgagatcct ggttgcctgc
     8581 gatcacgtcg cgcgctccgt cgtccaggac ggtcgtggaa tcgagcttcg gcaggcccgc
     8641 ggagccggtg cgcaattgtt cgacgcgatt ggcgctgtca gtgctcgtga acgccgtctg
     8701 cgcggtgttc tgcgacacgg ccggactcca tcccgacacc gtccccgtcg tcgaggcgaa
     8761 gaggaacacg gccggtccgc tgagcgcgcc ggacatcacc tccagcaccg ccttggacag
     8821 cggggcgccg cccacgtcgc cagagaggtc gcaggccagg taggcatcgg cgcgggcggg
     8881 gagagccagc ccggccgcca ggaaagaggc gatgagtccg gtgagaattc tcatgagagg
     8941 tctccaccag atcgacgaat gaaaagaagt gtccgtggac gggagatcct aacgccggtt
     9001 ggcgtttctt ccaatttccc gcaatgcgga cacgggagcc atagccggtg actgtcggcg
     9061 cgcttgtctt ttcctgtcgt gccaacgtcc aggaaatacg gggtctgccc tcactccgcg
     9121 cggagggccc tggcgggatc gatgcgcgtg gcgcgcagcg ccggcagcag gctcgcgagc
     9181 atcgccacgg cgccgagccc gcaggtcgtg acgaggagga tcgtcggatc cgccggacgc
     9241 acctcgaaca gcagccccga gacggcgcgc gacagggcca ccgcgccgag caggcccgcg
     9301 gcgagaccgg cggccgccag acgcgccgag cgggcgagaa ccctccgcag cacgtgcgcc
     9361 cggcgcgcgc cgagcgccat ccggatcccg atctccttcg tccgggtggc gacgatccct
     9421 gcgatgagcg cgaacagtcc cgacgccgcg aggagcaccg cggcggcggc gaacatcgcc
     9481 atgagcatga ggttgaagcg gcgcagcgcc acggcggcgg agaggatctc cggcatcgtc
     9541 cgcacgcccg aaagcgagat gccccggtcc acccccgcga gcgcttcgcg aacctcgtgc
     9601 tcgagcgcga gcgggtcggc gtccgcgctc acgaccaggt gaacccggcc ggtgaaattc
     9661 cccagggccg ccggcggaag ctgcgtgaac gggacgtaga tcgtcggcgc cggcggctcc
     9721 tcgagagccg cgagccggac cggcccgacc acccctatca cctcgacgtc gcgccagccg
     9781 ccgccgtcgt ccaggctgag atgcgctccg accgggtcct ccgcggggaa gtagcggcgt
     9841 gccagcgcgg cgtcgaccac ggccacgccg cgcgcccgcg cgtcgtccgc cgcggtgaac
     9901 gggcgaccgg cgatcaccgg aatccccatc acgtcgaaat acccggggct gatgacgcgg
     9961 ttctgcgccg aaggggtctc ggccggcgtg gccggcgggt ttcccgcgat ggtgaagtcg
    10021 gcgcgggaca acacgttggt cagcggcagc accgagatca gggccgcgcc ccgcacgccg
    10081 ggaagcgcca ggagccgtcg ctcgaggtcg gtgtccagcg cggcgagccg gtccacggtt
    10141 ccgtagccgc ctgcggtcag gccgagccgc gcgctcagaa cccccgcggg acgaatcccc
    10201 ggatcgacgc gcatcaaccg cgcataggac ttcaggaaga gtcccgcgcc cacgaggagc
    10261 gtcagcgaga tcgccacctc ggcgacgacg agcactccct gcgcccgggc ggcatccggg
    10321 ccgactcccg ccgagcgttc ggtgccgatc aggccggacg acgtccccct ccgcgacgct
    10381 ctccaggcgg gagccaggcc ggtcgcgagt ccggcgagga tcgtcgcccc gaacgcgaag
    10441 agcagcatcc gccagtccag ggagacttcg gccacgcgcg gcaggtccga cgggctcaac
    10501 gcgaggagtc ccgtgagccc ccacgcggcg agcagggcgc ccagggcgcc gccgagaaga
    10561 gccagcagca tgctctccgt caggagcagg cgcagcagcc ggccccgcgt cgctccgagg
    10621 gcggaccgca cggcgatctc gcgacgccgt ccggcggtgc gggcgacttg cagcgcggcc
    10681 aggttcgcac aggcgatcag gagcaccgcc gccacggaac cgagcaggag caggagagcg
    10741 ctccgatacg ggccgctcac ctcctcgcgc atcgatcgca cgcgcggcgc caggttcccg
    10801 gcgttctcgt ccgggtagcg ttcgcgcagg cgcgcggcga tcgttgccat ctccgctccg
    10861 gcctgttcgg gtgtgacccc cgcccgcagc ctaccgaagg cccgcaggaa attcgcgccg
    10921 cggttctcgc ggcgcgggtc
//
LOCUS       DASRIQ010000453         5511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1496277, whole genome shotgun sequence.
ACCESSION   DASRIQ010000453 DASRIQ010000000
VERSION     DASRIQ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496277"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VFD06_11055"
     CDS             <1..1365
                     /locus_tag="VFD06_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZN04108.1"
                     /translation="APAPAADLWPADAEPDLTNADVAIARTFPEFESRPAVRECETLL
                     LDAIGAARRSLYIESQYFTSEKLADAVAARLREPDGPEVLVVVPRECEGWLERNTMGA
                     FREGVFRRMAAANAHGRLRLLYPMASRAREIPTFIHSKVLVADDVLLRIGSANLARRS
                     MGLDTECDLAVDGSGDSGARAGIRLVRDRLLGEHLGLTAEEVAAEVARAGSLRQLVDG
                     RAGEDRTLVRIDPATLEEPSLPDAVKAAADPDGPVALDTVLEAVPQVRGSRLAGAGVS
                     ALAFVALFPVGLLATAAGLLLGAVLGIPVAFAGALLLAAIGHAAGRLLGGVGLARWMT
                     RRAYRSARQLGAKGVRGVLSLRLASIANGGSVHLFCGARRIPFGPYLLGSALALAPQV
                     VALAGLGALLRQAALQPSAARGVMVLATVLLLLVVSVLLRSALLTRRYAPSLERHRNQ
                     AEFG"
     gene            1365..2165
                     /locus_tag="VFD06_11060"
     CDS             1365..2165
                     /locus_tag="VFD06_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZN04109.1"
                     /translation="MPPESAVSSAPDPIAPIRVATYNVHGCIGRDGRHDPERVASVIV
                     ELDADIVALQEFTYPASIALESRSPAVLTTLDRYVCALGPTRQMPWAHGPERRVVTHC
                     FGNALFTRHPIREVHRIDLSHRRREPRGALAATIEVDGIAVHVLAAHLGLRLSERRFQ
                     VRQILDYLDSVRDTLFVVLGDFNDWLPGRSVVHLLDRRLGRPPRAPSYPVTWPVVSLD
                     RIWVHPARALRRVFCHRTPAARLASDHLPVVAEVDLASRPHGTDIAAS"
     gene            2162..2701
                     /locus_tag="VFD06_11065"
     CDS             2162..2701
                     /locus_tag="VFD06_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04110.1"
                     /translation="MTTRFRMRAALPAAFLALALTVLFVPEATAIWVPQTRAEFVDAV
                     AKGTKGAKMESFVVNRGFDEVVRTLEARCTPCLDVKVERVANVGYVERSSSDYNPELK
                     RVGKDRAEFTMQVIHRPRGVGHTPPPNGLYVMAADFRRAGVGKTEMILYRPVMGFKNI
                     SKALLAWAEGTTTDCPKMK"
     gene            3013..>5511
                     /locus_tag="VFD06_11070"
     CDS             3013..>5511
                     /locus_tag="VFD06_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN04111.1"
                     /translation="MLLLAAAGIRGAAAATLPYAMNLPCGQGAGNVGTWWISLPNVNP
                     TINGTPVVTAEDLCALIPNATSVSQAYGSGVGGGAVLGHEWTFDCLAQSCSASTLTPP
                     PPEAGCCSSSCFCVNPGEGYRVTVSAPSSFTISGSETPDLLKIPGGARDYLISVPFNS
                     CLTTAADLANATGLPSTGVQRGSVTALNGCTGSVISCNAGTAACTALTLVPGMGYRIR
                     YLDSLVHAYTNPVAGDFDADGLANCAENCPTIANPLQENGDGDGRGDACDNCPAVSNS
                     SQVDLDADGRGDACDNCPLSPNFIQTDGDIDLRGDVCDNCPANPNAGQSDVDRDGSGD
                     ACDICAGGGSRNVAIVDSASCANGGALPTSGVGETGSLATYSYFTVPVGGVTASQLAP
                     GGACGAEGCDTVLLNVCSAGMACTTAGLSAAEKADLGAFVGDGHKLIIYDSECPGVDY
                     SWLPIPFTTSPLNTAAVPGTVGIVEENSLSSSQPTESHFIDTSILGGVCTDDHVQSAN
                     VLQTQNAGWCVDMSATKGASGPVHLYATYGSSVSAIGLIIYNGLDLDGSCGDIPGTLT
                     ACQNFSKIWLQELQQPVDPSCLPCEQHVVSCDDENPCTDDVFNPFNGGCTHTPNFNPC
                     DDGNACTTGDTCSFNNCVGGPPVTCTASDQCHVAGVCDPGTGLCSNPEAPNGTACSDG
                     DACTHTDVCQSGVCTGSHEDICNCLDDDCDGTVDEDCLVKVGGAPIFPIPGGTLSSGF
                     SAYRLVPGPPHSNSGITTTDSATGLKVKSTLVQTLCASGNTAMFTGTCKSGLAYPLVT
                     PCTFSAVVVDGVLDEFTITVPEPPAQYGPVSLPPQ"
BASE COUNT          791 a         2010 c         1815 g          895 t
ORIGIN      
        1 gccccagccc ctgcggccga tctctggccg gccgacgccg agcccgatct caccaacgcg
       61 gacgtggcga tcgcccgcac gttcccggag ttcgagtccc ggccggcggt gcgcgagtgc
      121 gagacgctgc tcctcgacgc catcggcgcg gcccggcgct ccctctacat cgaaagccag
      181 tacttcacga gcgagaaact cgcggacgcc gtggcggcgc gcctgcgcga gccggacggc
      241 ccggaggtcc tcgtcgtcgt cccgcgcgag tgcgaggggt ggctcgagcg caacaccatg
      301 ggggcgtttc gggagggggt gtttcgccgc atggccgcgg ccaacgcgca cggccggctg
      361 cgattgctct atccgatggc gtcgcgggcg cgcgagattc cgactttcat ccattcgaag
      421 gtgctggtgg ccgacgacgt gctcctgcgc atcggctcgg ccaacctcgc gcgccgcagc
      481 atggggctcg acaccgaatg cgacctcgcg gtggacggct ccggcgattc cggtgcgcgc
      541 gcggggatcc gtctcgtccg cgaccgactg ctcggtgagc acctcggcct gaccgcggaa
      601 gaggtcgctg cggaagtggc ccgcgccgga tcgctgcgcc aactcgtcga cgggcgcgct
      661 ggcgaggacc ggaccctcgt ccggatcgat cccgcgacgc ttgaggagcc gtcccttccc
      721 gacgcggtga aggccgccgc cgatcccgac ggcccggtcg cgctcgacac ggtcctcgag
      781 gcggtcccgc aggtgcgcgg ctcgcggctc gccggagcgg gggtgtcggc gctggcgttc
      841 gtcgctctct tcccggtggg gctcctggcg acggcggccg gcctgctcct cggggcggtc
      901 ctcggcatcc cggtcgcttt cgccggcgcg ctcctcctcg ccgcgatcgg ccatgccgcc
      961 ggaaggttgc tcggcggcgt cgggctggcg cggtggatga cccggcgcgc gtaccgctcg
     1021 gcccggcagc tcggggcgaa gggagtccgc ggggtgcttt ccctgcgcct cgcctcgatc
     1081 gcgaacggcg ggtcggtgca cctgttctgc ggcgcgcgcc ggatcccctt cggtccgtac
     1141 ctcctcggct cggcactcgc cctcgctcct caagtcgtgg cgctcgccgg cctcggagcg
     1201 ctcctgcggc aggcggccct ccagccgtcg gcggcccgag gggtgatggt gctggcgacg
     1261 gtcctgctcc tgctcgtcgt ctccgtcctg ctgcggtcgg cgctgctgac gcgccgttac
     1321 gcgccgtcgc tcgagcgcca ccgcaaccag gcggagttcg gctgatgccg ccggaatccg
     1381 cggtctcttc ggcgcccgat ccgatcgcgc cgatccgcgt cgcgacctac aacgtccacg
     1441 gctgcatcgg gcgcgacggg cgccacgatc ccgagcgtgt cgcgagcgtc atcgtcgagc
     1501 tggatgccga catcgtggcg ctccaggagt tcacctatcc ggcgagcatc gcgctcgagt
     1561 cccggagccc cgccgtcctc accaccctcg accgttatgt ctgcgcgctc ggtccgaccc
     1621 ggcagatgcc atgggcgcac ggtccggagc gtcgggtggt gacccactgt ttcggcaacg
     1681 ccctcttcac ccgccacccg atccgggagg tccatcgcat cgacctgtcg caccggcgcc
     1741 gcgagccgcg cggcgccctg gcggcgacca tcgaggtgga cgggatcgcg gtgcacgtcc
     1801 tcgcggcgca cctcgggctg cgcctgagcg agcggcgctt ccaggtgcgg cagatcctcg
     1861 actacctcga ttcggtgcgg gacaccctct tcgtcgtgct gggggacttc aacgactggc
     1921 ttcccgggcg gtcggtcgtg cacctgctcg accggcgcct cggccgcccg ccgcgcgccc
     1981 cgtcctatcc cgtgacctgg ccggtcgtct cgctcgatcg catctgggtc cacccggccc
     2041 gcgcattgcg ccgcgtcttc tgccaccgca cgccggccgc ccggctcgct tccgatcacc
     2101 ttcctgtagt cgcggaggtc gatcttgcct cgcgccccca cgggaccgat attgcggcct
     2161 cgtgacgaca cggttccgga tgcgcgcggc ccttccggcc gcgtttctcg cgctcgcgct
     2221 gacggtcctc ttcgtcccgg aggcgactgc catctgggtc ccgcagaccc gcgccgagtt
     2281 cgtggacgcg gtcgccaagg ggacgaaggg cgcgaagatg gagtcgttcg tcgtgaatcg
     2341 cggcttcgac gaagtggtcc ggacgctgga ggcgcgctgc acgccgtgcc tcgacgtcaa
     2401 ggtcgagcgg gtcgcgaacg tcggatacgt ggagcgatcc tcctccgact acaacccgga
     2461 actgaagcgc gtcggcaagg accgggccga gttcaccatg caggtgatcc atcggccgcg
     2521 cggcgtcggc cacaccccgc cgcccaacgg tctctacgtc atggccgccg acttcaggcg
     2581 cgccggcgtc ggcaagaccg aaatgatcct ctaccgcccg gtcatggggt tcaagaacat
     2641 cagcaaggcg ctcctggcat gggcggaagg aacgacgacc gactgcccga agatgaagta
     2701 acggcgcccg ccgctcgcga tcggctcccc gaaaaactga tcagcaattc ttccgatcgg
     2761 tgatcgcgat cacagaaact tcttgacacg ctgatgaagt gcccgtagat tcgcgcacag
     2821 ttccggggtt cactcgtcgc aactcgctcc actcgtcggc aactcgcaat actcgtcggc
     2881 aactcgcaac agcagcactc agcgccgctt ttctgggacg gacccatgca tcctcatcgg
     2941 atgattcggg accggtctgg gcagggagta tctcgcgcgc gcacggtcaa acgggcgctg
     3001 gcggggctgg cgatgctgct cctcgctgcg gcgggtatcc gcggcgcggc ggccgcgacg
     3061 ctcccctacg cgatgaacct gccctgtggt cagggcgcgg ggaacgtcgg cacctggtgg
     3121 atctcgctgc cgaacgtcaa cccgacgatc aacggcaccc ccgtcgtcac ggccgaggac
     3181 ctctgcgcgc tgatcccgaa cgccacctcc gtgtcgcagg cgtacggctc gggggtcggc
     3241 ggcggcgccg tcctggggca cgagtggacc ttcgactgcc tggcgcagag ctgcagcgcc
     3301 agcaccctca cgccccctcc gccggaagcg ggctgctgct cctcctcctg tttctgcgtc
     3361 aatccaggag agggatacag ggtcacggtg agcgccccct cgtcgttcac gatctccggg
     3421 agcgagacgc ccgacctcct caagatcccg ggaggagccc gcgactacct catctcggtt
     3481 cccttcaaca gctgcctcac caccgccgcc gacctcgcga acgccacggg ccttccgagc
     3541 accggagttc aacgtgggtc tgtcaccgcc ctgaatgggt gcacgggtag tgtgatcagt
     3601 tgcaacgccg ggacggccgc gtgcaccgcg ctgacgctcg tcccggggat gggctatcgg
     3661 atccgctatc tcgactcgct cgtgcacgcc tacaccaatc ctgtggccgg cgacttcgac
     3721 gccgacggcc tcgcgaattg cgctgagaac tgcccgacga ttgccaatcc tctccaggaa
     3781 aacggggacg gtgacgggcg cggcgacgcc tgcgacaact gtccggccgt ctccaattcc
     3841 tcccaggtcg acctcgacgc ggacggccgg ggggatgcgt gcgacaactg tccgctgtct
     3901 cccaacttca tccagacgga cggcgacatc gatctgcgtg gcgatgtctg cgacaactgt
     3961 cccgcgaacc cgaacgccgg ccagtcggac gtcgaccggg acggttccgg agacgcctgc
     4021 gacatctgcg ccggtggcgg ctcgcgcaat gtcgcgatcg tcgattcggc ctcgtgcgcc
     4081 aacggcgggg ctcttccgac ttcgggggtc ggcgagaccg ggtcgctcgc gacctacagc
     4141 tatttcaccg tcccggtcgg cggcgtgacc gcgtcgcagc tcgcccccgg cggagcgtgc
     4201 ggcgccgagg ggtgcgacac ggtgctcctg aacgtctgca gcgccgggat ggcctgcacg
     4261 accgcggggc tctccgccgc cgagaaagcg gacctcggcg cgttcgtggg ggacgggcac
     4321 aagctcatca tctacgacag cgaatgcccc ggcgtggact actcctggct gccgattccc
     4381 ttcaccacct cgccgctcaa caccgccgcc gtcccgggaa cggtcggcat cgtcgaggag
     4441 aactccctct cgtccagcca gccgaccgag agccacttca tcgacacctc catcctcggc
     4501 ggggtgtgca ccgacgatca cgtccagagc gccaacgtgc tgcagacgca gaacgccggc
     4561 tggtgcgtcg acatgagcgc gaccaagggc gcctccgggc cggtgcacct gtacgccacc
     4621 tacgggtcga gcgtctcggc catcggcctc atcatctaca acgggctcga cctcgacgga
     4681 tcgtgtggcg acatccctgg gaccctgacc gcctgccaga acttctcgaa gatctggctc
     4741 caggagctgc aacagccggt cgatccatcg tgcctgccat gcgagcagca tgtggtgtcg
     4801 tgcgacgacg agaatccgtg cacggacgac gtcttcaatc cctttaacgg cggctgcacc
     4861 cacacaccga acttcaatcc gtgcgacgac ggcaacgcct gtacgacagg cgacacgtgt
     4921 tcgttcaaca attgcgtcgg cgggccccca gtcacctgta ccgcgagcga ccagtgccac
     4981 gtcgccggcg tctgcgaccc ggggaccggc ctgtgctcga acccggaggc gccgaacggc
     5041 acggcgtgca gcgacggcga cgcctgcacg cacacggacg tctgtcagtc gggggtctgc
     5101 accgggtcgc atgaggacat ctgcaactgc ctcgatgacg actgcgacgg caccgtcgac
     5161 gaggattgcc tcgtgaaggt cgggggcgcc cctatcttcc ccatccccgg cgggactctt
     5221 tcgtccgggt tttccgctta tcgtctcgtt cccggaccgc cccatagcaa tagcgggatc
     5281 accacgaccg acagcgctac cgggctgaag gtgaagtcga ccctggtcca gacgctctgc
     5341 gcctcgggaa acaccgccat gttcacgggg acgtgtaaga gtggcctggc ctacccgctg
     5401 gtgacgccct gcaccttctc agcggtggtg gtggacggcg tgctcgatga gttcaccatc
     5461 accgtccccg aaccgccggc gcagtacggt ccggtctcgt tgcccccgca g
//
LOCUS       DASRIQ010000454         5116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2258763, whole genome shotgun sequence.
ACCESSION   DASRIQ010000454 DASRIQ010000000
VERSION     DASRIQ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258763"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..1016
                     /gene="mqnC"
                     /locus_tag="VFD06_11075"
     CDS             12..1016
                     /gene="mqnC"
                     /locus_tag="VFD06_11075"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HZN04112.1"
                     /translation="MALAADAVRRRLHPAPIVTYIVDRNINYTNVCTARCSFCAFYRE
                     PGDPEGYLHDKETLFAKIEETLRLGGTGILMQGGHHPDLPIDWYEDLLRSFVARFPTL
                     HIHAFSPSEIQHVSRVSRLTIAETLRRLQAAGLHSIPGGGGEILVDEVRRTISPLKTM
                     SADWLEVMETAHALGLPTTATMMFGHVESYADRVAHFEKIRSVQDRSRALGATGFTAF
                     ISWTYKAEHTALGGREATSAEYLRTQALSRLYFDNIPNIQSSWVTQGKEIGQVALRFG
                     ANDMGSIMIEENVVKAAGAHGCTTRAEMERLIREAGFAPRQRDTLYRLVEEPARIPST
                     "
     gene            1022..1363
                     /locus_tag="VFD06_11080"
     CDS             1022..1363
                     /locus_tag="VFD06_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04113.1"
                     /translation="MSGATIVLAMLLTAVVVVGAASIGSIRCALGREAIEVRVFDRVV
                     RRVRLSDIESVDRRGAIVNESWSGPRFWNAVTLRRRTGLLRNVIVTPDDPDRFVAEVT
                     ERLREGPGPSG"
     gene            complement(1335..2450)
                     /gene="amrS"
                     /locus_tag="VFD06_11085"
     CDS             complement(1335..2450)
                     /gene="amrS"
                     /locus_tag="VFD06_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZN04114.1"
                     /translation="MKDATAPLARTLSALTRPGTLYDPLPDGAVRCYACGHNCLIPKN
                     RPGVCKVRFNDEGVLQVPWGYVGALQIDPIEKKPFFHAYPGTEALSFGMLGCDYHCSY
                     CQNWITSQAIRDPAAQSAPRPITPDELTALAVGHGTPVMVSTYNEPLITSEWALAIFR
                     EARRAGIVAGYVSNGNGTERVLDALQPHLTLYKIDLKGFDDRAYRKLGGTLQAVQRTI
                     ESVHRRGLWLEVVTLLVPGLNDSDAELRAAAGFLRDLSPDIPWHVTAFHADYRMTEPP
                     ATGAETLLRAASIGRKEGLRYVYAGNLPGRVGDLEDTRCSSCDATVVARRGFRVLRVD
                     LSEGGTCRRCDAPVPGVWSAAARHPARPLSRTARDLP"
     gene            2679..3311
                     /locus_tag="VFD06_11090"
     CDS             2679..3311
                     /locus_tag="VFD06_11090"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN04115.1"
                     /translation="MARLRACRNVESTRSVLVHIPIDRAWELVRDFPALFAAHGRGRP
                     LLRIEAIRLDRGDGRSAASLWRQRGTWAGRPYWAEIEILESAPPHALAIRLVRDSLGS
                     ERGLLGHRGEIRLRAEGEGMRSTKITWHLTARLRSLPMLLTQVLVPERARTRLLDLSL
                     RSLKVAIDGGTRPAEAWPTTPFPIPTRAATVPSVPRGPSARTSVRRRQRP"
     gene            3335..4234
                     /locus_tag="VFD06_11095"
     CDS             3335..4234
                     /locus_tag="VFD06_11095"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HZN04116.1"
                     /translation="MHRARRSTVLLILAVLSGPIAAAVRSDPREPRWLNGLLLEARGD
                     RVVVTRVLPGSPAASAGLLAGDVLLVVDDQIVSDLQPTPPVEMLRRLDRLVEERRNTS
                     LRLVVGRGGRTLGVMLPTGLPLATVTRGALEAGQPAPPFSGKDQDGRTVESGALRGRI
                     VLIDFWASWCPPCRDAALVVRRLAAQHGERLAVVGVSVDEDPRAYEAFVYNQHLPGRQ
                     IHDGGPAGPISSAYGAPAAGLPFSVLIAADGTIAALGRSPAEVEPALERLLAALPGRD
                     AARDATGGTSAPHAGTGSGDDPS"
     gene            4279..>5116
                     /locus_tag="VFD06_11100"
     CDS             4279..>5116
                     /locus_tag="VFD06_11100"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HZN04117.1"
                     /translation="MVALAVAVGPLRAGADAPPSDPSFGDPVPLRGQMPSGLPFLELP
                     PASAFLLSRGAFRFSVGLAYENTHAVSDDLVRTYNDGARHLLTLSDLESIAAGSKEGT
                     AYLVDGETLRLALGGAFGLGARLEAAFELPILLHTGGFMDGTIEGYHDLLGFGDGGRS
                     AFEQDRYIVAYADPDGTFYLEGAPDGIRPGDLALMLRGSLFQSPTGGSALAATVGIEI
                     PTGDADRLEGNGAFDAAFGIEASWRFRRSSMHAGGRYARPGGWDAAPWVDLGGRLGGF
                     AAW"
BASE COUNT          694 a         1863 c         1799 g          760 t
ORIGIN      
        1 ccctcaccga cctggccctc gccgccgacg cggtgcgacg ccggctgcac cccgcgccga
       61 tcgtcaccta catcgtcgat cgcaacatca actacaccaa cgtctgcacg gcccgctgtt
      121 ccttctgcgc cttctaccgc gagccggggg atccggaggg gtacctgcac gacaaggaga
      181 ccctgttcgc gaaaatcgag gagacgctcc ggctcggcgg aaccggcatc ctcatgcagg
      241 ggggacacca tcccgacctg ccgatcgact ggtacgagga cctgttgcgc tccttcgtcg
      301 cgcgcttccc gaccctgcac atccacgcct tctcgccgtc ggagatccag catgtctccc
      361 gcgtgtcgcg cctgacgatc gccgagacgc tccgccggct gcaggcggcc gggctgcaca
      421 gcatcccggg agggggcggc gagatcctcg tggacgaggt gcgacggacg atctcgccgc
      481 tcaagacgat gtccgccgac tggctcgagg tcatggagac ggcccacgcg ctcgggctcc
      541 cgaccaccgc gacgatgatg ttcgggcacg tggagtcgta cgccgaccgg gtggcgcact
      601 tcgagaagat ccggagcgtt caggacagga gccgcgctct cggggcgacc ggtttcacgg
      661 ccttcatctc ctggacctac aaggcggagc acaccgccct cggcggccgc gaggcgacca
      721 gcgccgaata cctgcgcacc caggccctca gccgcctcta cttcgacaac atccccaaca
      781 tccagtcctc ctgggtcacg cagggcaagg agatcgggca ggtggcgctg cgcttcgggg
      841 ccaacgacat gggcagcatc atgatcgagg agaacgtggt gaaggcggcc ggggcgcacg
      901 gctgcaccac gcgcgcggag atggagcggc tcatccgcga ggccggcttc gccccgcgcc
      961 agcgggacac gctctaccga ctcgtcgagg agcccgcccg gatcccctca acctagccgc
     1021 gatgagcggc gcgaccatcg tcctggcgat gctgctgacc gccgtggtgg tcgtgggggc
     1081 cgcctccatc ggatcgatcc gctgtgccct cgggcgcgag gcgatcgagg tgcgggtctt
     1141 cgaccgggtg gtgcgtcggg tccgcctctc cgacatcgag tcggtggacc ggcgcggggc
     1201 gatcgtcaat gaaagctggt caggcccgcg cttctggaac gcggtcacgc tgcgccggcg
     1261 caccgggctg ctccgcaacg tcatcgtcac gccggacgat cccgatcgct tcgtggccga
     1321 ggtgaccgag cgcctcaggg aaggtcccgg gccgtccggc tgagcgggcg cgcgggatgc
     1381 cgggcggccg cggaccagac gcccgggacc ggcgcgtcgc agcggcggca cgttcccccc
     1441 tccgagagat cgacccggag cacccggaag ccgcgccgcg cgacgacggt cgcgtcgcac
     1501 gaagagcagc gcgtgtcttc gaggtcgccg acccgccccg gcaggttccc ggcgtagaca
     1561 tagcgcagcc cctccttccg gccgatcgac gccgcgcgca gaagcgtctc ggccccggtc
     1621 gcgggcggct ccgtcatccg gtagtcggcg tggaaggcag tgacgtgcca cgggatgtcc
     1681 ggcgagagat cgcgcaggaa gccggcggcc gcgcgcagct ccgcgtccga gtcgttcagg
     1741 ccggggacga ggagcgtcac cacctcgagc cacagcccgc gccggtgcac cgactcgatg
     1801 gtccgctgca ccgcctgcaa ggtcccgccg agcttccggt aggcgcgatc gtcgaagcct
     1861 ttcagatcga tcttgtagag ggtcaggtgc ggctggagag cgtcgagcac gcgctcggtc
     1921 ccgttgccgt tgctgacgta gccggcgacg atcccggccc gccgcgcctc gcggaaaatc
     1981 gccagggccc actcggaggt gatcagcggc tcgttgtagg tggaaaccat caccggggtc
     2041 ccgtgcccga ccgccagcgc ggtcaactcg tccggcgtga tcgggcgggg cgccgactgc
     2101 gccgcgggat cgcggatcgc ctgcgaggtg atccagttct ggcagtaaga gcaatggtaa
     2161 tcgcagccga gcatgccgaa ggagagggcc tcggtcccgg ggtacgcgtg gaagaacggc
     2221 ttcttctcga tcggatcgat ctgcagggct ccgacgtacc cccagggcac ctgaaggacc
     2281 ccttcgtcgt tgaagcgcac cttgcagacg cccggacggt tcttcgggat gaggcagttg
     2341 tggccgcagg cgtagcagcg caccgctcca tccgggagcg ggtcgtacag cgtcccggga
     2401 cgggtcaggg cggaaagggt ccgggccaga ggagcggtcg cgtccttcac ggcaccccga
     2461 gtataggggc ctgtccgcca ggctcccagt cctgccacac cttcagggca acgaggcgct
     2521 ggacctgtgc taggcttccg caccgctcgg caccccggga gcgatcgccg ccaaaaacct
     2581 cagtgacact tcgcgggcgc ggcccgcgtc gagagaggtg tcgcgtgctg atgttggtga
     2641 cggcggccgt ggttttcgga ggcctggcga ttctcgtctt ggcgaggctc cgtgcctgcc
     2701 ggaacgtcga gtcgacccgc tccgtcctgg ttcacatccc catcgatcgc gcctgggagc
     2761 tcgtccgcga tttcccggcg ctgttcgcgg cgcacggccg cgggcgcccg ctcctgcgca
     2821 tcgaggcgat ccggctcgac cgcggcgacg gtcgaagcgc cgcgagtctc tggcggcagc
     2881 gcggcacctg ggccgggcgt ccctactggg ccgagatcga gatcctcgag tccgccccgc
     2941 cgcacgccct ggcgatccgg ctggtgcgcg actcgcttgg gtccgagcgc gggctcctgg
     3001 gccaccgcgg cgagatccgc ctgcgcgctg agggtgaggg gatgaggtcg acgaagatca
     3061 cctggcacct caccgcgcgg ctccgcagcc tgccgatgct cctgacgcag gttctcgtgc
     3121 cggagcgggc gcggacgcgt ctcctcgacc tgagcctgcg ctccctgaag gtggcgatcg
     3181 acggcgggac gcggccggcc gaagcgtggc cgacgactcc cttcccgatc ccgacccggg
     3241 ccgcgacggt cccgtcggtg ccgcgggggc cctccgcgcg cacctcggtc cggcggcggc
     3301 agcggccctg atcgggtgct acgatcgcgg cgcgatgcat cgcgcgcgac gctcgaccgt
     3361 ccttctgatc ctcgcggtcc tttccgggcc gatcgccgcg gccgtgcgca gcgatccacg
     3421 ggaaccgcgc tggctgaacg gtctcctcct cgaggcacgc ggcgaccgcg tcgtggtgac
     3481 gcgcgtcctc ccggggtctc cggccgcctc ggccggcctc ctggccggcg acgtgctgct
     3541 ggtcgtcgac gatcagatcg tctccgacct gcagccgacc ccgccggtcg agatgctccg
     3601 tcgcctcgat cggctcgtcg aggagcggcg caacacctcc ctccggctcg tggtcgggcg
     3661 gggaggtcgc acgctcggcg tgatgctgcc gaccggcctg cccctagcga ccgtgacgcg
     3721 cggggcgctc gaagccggcc agccggcgcc gccgttctca gggaaggacc aggacgggcg
     3781 cactgtcgaa tccggcgcgc tccgtgggcg gatcgtcctg atcgacttct gggcctcctg
     3841 gtgccccccc tgtcgcgacg cggccctggt cgtccgccgc ctcgcggcgc agcacggcga
     3901 gcggctggcc gtcgtggggg tgagcgtcga cgaagacccg cgcgcctacg aggcattcgt
     3961 ctacaaccag cacctgcccg gaaggcagat ccacgacggc ggtccggcgg gaccgatctc
     4021 ctcggcgtac ggcgctccgg ccgccggcct ccccttctcg gtgctcatcg ccgccgatgg
     4081 cacgatcgcc gccctggggc ggtcccccgc cgaggtcgag ccggcgctcg aacggctgct
     4141 cgccgccctc cccgggcgcg acgccgcccg cgatgccacc ggcgggactt ccgctccgca
     4201 tgccggaacc ggctccggcg acgatccctc ctgaggattc cgaggagtcc ccggggtgcg
     4261 cggattgccg cggcgtgcgt ggtcgctctc gccgtggcgg tcggtcctct ccgcgccggg
     4321 gcggacgccc ccccgtccga tccctcgttc ggcgatccgg taccgctgcg cggccagatg
     4381 ccgagcggcc tgccgtttct cgagctcccc cccgccagcg cctttctcct ctcgcgcggc
     4441 gcgttccgct tcagcgtcgg cctcgcgtac gagaacacgc atgcggtcag cgacgatctc
     4501 gtacgcacct acaacgatgg cgcacggcac ctcctgaccc tctccgacct cgaatcgatc
     4561 gccgcagggt cgaaggaagg gaccgcgtac ctcgtcgatg gagagacatt gcggctggcg
     4621 ctcggcgggg cgttcggcct cggggcgcgt ctcgaagccg ccttcgagct gccgatcctc
     4681 ctgcacacgg gagggttcat ggacgggacc atcgagggat atcacgatct cctcggcttc
     4741 ggggacggag ggcgatccgc gttcgagcag gatcgctaca tcgtcgccta cgccgatccg
     4801 gacgggacct tctacctcga gggggcgccg gacggcatcc gccccggcga cctggcgctg
     4861 atgctgcgcg ggtcgctctt ccagagcccc accggcggat cggcgctcgc cgccacggtc
     4921 ggcatcgaga ttccgaccgg cgacgccgat cgtctggagg ggaacggggc gttcgatgcc
     4981 gccttcggca tcgaggcgtc gtggcggttc cgccggagct ccatgcacgc cggggggcgt
     5041 tatgcgcgcc cggggggctg ggacgccgcc ccatgggtcg acctgggcgg acgcctcggg
     5101 ggattcgccg cctggg
//
LOCUS       DASRIQ010000455         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1497028, whole genome shotgun sequence.
ACCESSION   DASRIQ010000455 DASRIQ010000000
VERSION     DASRIQ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497028"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VFD06_11105"
     CDS             complement(<1..901)
                     /locus_tag="VFD06_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04118.1"
                     /translation="MLLDWATISYRSIMRSVVLVVVLAAAGGAFFWFRSSLKGSPEEL
                     ALVDIGRAERLYREAQAAAGADNDRVAAVGRVGRLLNDARLAYDRRNYPEARAAAQQS
                     QTFSEKLLEGQQGESFAARIFKFEGDVKVKRAREFVWASLSGNTALRVGDQIKTASSG
                     SAQIIYFDGTITTIKPGSLLEIRELFEDPATRVRKVEEKVNWGGISATTSDANVKGSV
                     HSILTDSTTTRAAGRAQFDVSFDATRRSARTEVHSGQTEMTAAGGTHTLRPLDRVEVQ
                     GERIVARATLPAAPSLLDPADSRV"
     gene            1159..1620
                     /locus_tag="VFD06_11110"
     CDS             1159..1620
                     /locus_tag="VFD06_11110"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZN04119.1"
                     /translation="MLQQMRKWSGYLKYLLFIVIFMFIVWGVTIWNGGSAGGSNAADE
                     WAADVNGTVIEGNAFRGYARRLDSNFQQMFGEQYAQQRSFFRVGRQAIQQMIDEELIL
                     QAARAEGLQVSPQEVADALTRDPQFQENGRFIGFDRYRAQFQQAGIAMPDY"
     gene            1627..3054
                     /locus_tag="VFD06_11115"
     CDS             1627..3054
                     /locus_tag="VFD06_11115"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZN04120.1"
                     /translation="MRRRLLGEKYRSLVTTSVTVSDAEVQEELKRRLQKTSVDWVLVE
                     PDALTVPGADDAALQAWYRGHEGQYRRGEGRTGRYVLFDPQSLAASATFSDAEVQAAY
                     DRDLATRYTAKEQRRASHILFKVAPDAPPGDAAKIEAKANDALKRAKGGEDFAALARR
                     FSEDSTASNGGDLNFFGRGQMAKEFEDASFGLPVGGISDLVKTRYGYHIIKVTDTRPG
                     RTIPFEEARDRIRQEMQVDRARALATERASSFAAVAKKDGFETAAKAAGLTVKDTGPV
                     RPGDSLADLPASQPAAQRMQAYKAGEVSEPIPVPEGQVVIEVTGTVPDEPRPFAEVRP
                     RVEQDWQVDRRRVLVAERLRNAGTLEAFAKSLKKEVRHQDDLAMGTPLPGIPPDEALS
                     RQIETLPPGALGDPVATSAGLVVLRVSKRDDGAAEFASQRDATRDSLMSQRRERLLRA
                     VVRRLQEQGEVRINEPMVDAVDRSS"
     gene            3130..3627
                     /locus_tag="VFD06_11120"
     CDS             3130..3627
                     /locus_tag="VFD06_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04121.1"
                     /translation="MSTRRAERAFEKGLQSIARGDFLAALGFFEASMALLPANGGTHA
                     VAAMSYYGLCLACATDRLPEARDLCEAAVEAVPEDPDLYLNLGRVCERQEDREHAFRT
                     YVRGLRLNPRHPGLVEALRRLGFRRRSVVGFLPRDHAVNRVLGSLRAALQRRPRRRTR
                     RPKAA"
     gene            complement(3614..>5045)
                     /locus_tag="VFD06_11125"
     CDS             complement(3614..>5045)
                     /locus_tag="VFD06_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HZN04122.1"
                     /translation="NTTLTLLATDGVTVLASNDDRGPSDKSSLVNYTPPSSARLYVRS
                     AHGLGFGLYGSYDLKLLGTAAGTDADQDGFPSTNDCNDANPSIYPGATETCNLVDDDC
                     DASVDEGFDQDYDGVTTCAGDCNNLNAAIHPGAPEVCNGIDDDCDTQIDEGAFPDTDG
                     DGVRDCVDPDDDADGTQDSEDCLPLSYLASNLPAEVLESAEIPAPGVARLRWGHVPQT
                     NVYNVYRATRTSLGASPGFSCAAAQQPGRQFDDAEAPPLGAVFVYVQAGANFCGEGSL
                     GSDSTGAPRAPTVACGAPGLDSDGDTVADLVDVCPLSQDPGQEDPDRDGRGTSCDNCF
                     AVANAGQQDADGNGSGDACQDGDVDGFPASTDCDDADPAVHPGATEIFNGRDDDCDGI
                     VDDVVAVVTVTLAQWQSSNSRLTVEATTNYPVGSVSLTVTGYGVMTWEPAASVYRLIV
                     QPTANPGSVTVTSTAGGSGTLPVTPL"
BASE COUNT          726 a         1778 c         1808 g          733 t
ORIGIN      
        1 agacccggct gtcggccggg tcgaggagcg acggggcggc cgggagcgtg gcgcgcgcga
       61 cgatccgctc cccctgcacc tccacgcgat cgagcggacg cagcgtgtgc gtccctccgg
      121 cggccgtcat ctcggtctgg ccgctgtgca cctcggtgcg ggcgctccgg cgggtcgcgt
      181 cgaaggagac gtcgaactgg gcgcgtcccg ccgcgcgcgt cgtcgtggaa tcggtgagga
      241 tcgaatggac cgatcccttg acgttggcgt cggaggtggt ggcggagatc ccgccccagt
      301 tgactttctc ctccaccttg cggacccgcg tcgcgggatc ttcgaacagc tcccggatct
      361 cgaggagcga tcccggcttg atggtggtga tggtgccgtc gaagtagatg atctgcgccg
      421 agccgctcga cgccgtcttg atctggtcgc cgacgcgcag cgccgtgttg ccggagaggg
      481 aggcccagac gaactcgcgc gcccgcttca ccttcacgtc cccctcgaac ttgaagatgc
      541 gggcggcgaa cgactccccc tgctgcccct cgagcagctt ctccgagaag gtctgcgact
      601 gctgcgcggc cgcgcgcgcc tccggatagt tgcggcgatc gtaggcgaga cgcgcgtcgt
      661 tgagcaggcg cccgacccgt ccgacggcgg cgacgcggtc gttgtcggcc cccgcggccg
      721 cctgcgcctc acggtacagg cgctcggcgc ggccgatgtc cacgagggcc agctcctcgg
      781 ggctcccctt cagcgacgag cggaaccaga agaacgctcc gccggcggcg gcgaggacga
      841 cgaccaggac gacgctccgc atgatgctgc ggtaggaaat cgtcgcccag tccagaagca
      901 cctggggctg ggagtcccgc cccggccgtc tccctggtcc cggtcccgtt cgtgccgcca
      961 cggttcgtcg cctcccagga gctcctcgac gaccgcggcc gggcaggagg gggcccggcc
     1021 ggacgagggg ccgaagggca atataggagg cgcgacgccc aaggcaagga ccccttgctc
     1081 cccccccgcc ccggccggta cactttgcgg ctccgggtgc ggtgctcccg cgtccccttc
     1141 cagaacgagg caggcgcaat gttgcagcag atgcggaaat ggtccggcta cctgaaatac
     1201 ctcctgttca tcgtcatctt catgttcatc gtctggggag tcacgatctg gaacggcggc
     1261 tcggccggcg ggtcgaacgc cgccgacgaa tgggcggccg acgtcaacgg caccgtcatc
     1321 gagggcaacg ccttccgcgg ctatgcccgc cggctcgaca gcaacttcca gcagatgttc
     1381 ggggagcagt atgcgcagca gcggtccttc ttccgcgtcg ggcgccaggc catccagcag
     1441 atgatcgatg aggagctgat cctccaggcg gcgcgcgccg agggccttca ggtgagtccc
     1501 caggaagtcg ccgacgcgct gacccgcgac ccgcagttcc aggagaacgg ccgcttcatc
     1561 ggcttcgacc gttaccgcgc ccaattccag caggccggca tcgccatgcc ggactactag
     1621 gacacggtgc gccggcgact gctcggggag aagtaccgga gcctcgtgac caccagcgtc
     1681 accgtctcgg atgccgaggt gcaggaggag ctcaaacgcc ggctgcagaa gacctcggtc
     1741 gattgggtgc tcgtcgagcc ggacgcgctg acggtccccg gcgccgacga cgcggcgctt
     1801 caggcctggt accgcgggca cgagggacag taccgtcgcg gcgaggggcg caccggccgc
     1861 tacgtgctgt tcgatcccca gtcgctcgcg gcgtcggcga ccttcagcga cgccgaggtc
     1921 caggccgcct acgatcgcga tctcgccaca cgctacaccg ccaaggagca gcggcgcgcg
     1981 tcgcacatcc tcttcaaggt cgcgccggac gccccgccgg gcgacgcggc caagatcgag
     2041 gcgaaggcga acgacgccct gaagcgggcg aaaggcggcg aggatttcgc cgcgctggcg
     2101 cgccgcttct cggaggacag caccgcctcg aacggcggcg atctcaattt cttcggacgc
     2161 gggcagatgg cgaaggagtt cgaggacgcc tccttcggcc tgccggtggg ggggatcagc
     2221 gacctggtga agacccgcta cgggtaccac atcatcaagg tgaccgacac gcgaccgggc
     2281 cgcacgatcc cgttcgagga ggcgcgcgat cgcatccgcc aggagatgca ggtggaccgg
     2341 gcccgggcgc tcgccaccga gcgggcgtca agcttcgctg cggtggcgaa gaaggacggc
     2401 ttcgagacgg cggcgaaggc cgccggcctg acggtgaagg acacggggcc ggtgcggccc
     2461 ggcgactccc tcgccgatct ccccgcctcg cagccggccg cgcagcggat gcaggcgtac
     2521 aaggccggcg aggtgagcga gccgatcccg gtccccgaag ggcaggtggt gatcgaggtg
     2581 accggcaccg tgcccgacga gccccggccg ttcgccgagg tgcgcccgcg cgtcgagcag
     2641 gactggcagg tggatcggcg cagggtgctg gtcgccgagc ggctgcggaa cgccgggacg
     2701 ctcgaggcgt tcgccaagtc cctcaagaag gaagtccggc accaggacga cctggcgatg
     2761 gggacgcccc tccccgggat cccgccggac gaggcgctga gccgccagat cgagacgctc
     2821 ccgcccggcg ccctgggaga cccggtcgcc acgagcgccg gcctcgtcgt cctccgcgtc
     2881 agcaagcggg acgacggcgc cgccgagttc gcctcccagc gcgacgcgac gcgcgacagc
     2941 ctgatgtcgc agcgccgcga gcggctgttg cgcgccgtgg tccggcggct ccaggagcag
     3001 ggcgaggtgc ggatcaacga accgatggtc gacgccgtcg atcgcagctc ctgatcgcac
     3061 ccctccacct gcaccgatcc cttgaatcgg ggagccggac aacctagatt tcggcgacgg
     3121 gggactcctg tgtccacgcg ccgcgccgaa cgcgccttcg agaaagggct gcagtcgatc
     3181 gcccgggggg acttcctcgc cgccctgggg ttcttcgagg cctcgatggc ccttctcccc
     3241 gccaacggcg ggacgcacgc ggtcgcggcg atgtcctact acggcctgtg cctcgcctgc
     3301 gcgaccgatc gcctgccgga ggcgcgcgat ctctgcgagg cggcggtcga ggcggtgccg
     3361 gaggatcccg acctctacct gaacctcggg cgcgtctgcg agcggcagga ggatcgcgag
     3421 cacgccttcc gcacgtacgt gcgggggctg cgactcaacc cgcgccaccc cggcctggtg
     3481 gaggcgctgc gccggctcgg gttccggcgg aggtcggtgg tggggttcct gccgcgcgac
     3541 cacgcggtga accgggtgct cggcagtctg cgcgcggcgc tgcagcgccg tccgcggcgc
     3601 cggacacgcc gccctaaagc ggcgtgaccg ggagggtccc ggatccgccg gcggtcgacg
     3661 tgaccgtgac gctcccggga ttggcggtcg gctgcacgat cagccggtac accgaggccg
     3721 ccggctccca ggtcatcacg ccgtagccgg tcaccgtcag cgagaccgat ccgaccgggt
     3781 agttcgtggt ggcctcgacc gtcaaccggc tgttcgagct ctgccactgg gcgagggtca
     3841 cggtcacgac cgcgaccacg tcatccacga tcccgtcgca gtcgtcgtcc cggccgttga
     3901 agatctccgt cgcccccgga tggacggccg gatccgcgtc gtcgcagtcg gtgctggcgg
     3961 ggaaaccgtc cacgtcgccg tcctggcagg cgtcgccgga tccgttgccg tctgcgtcct
     4021 gctgcccggc gttggcgacg gcgaagcagt tgtcgcagga cgtgccgcgc ccgtcccgat
     4081 cgggatcctc ctgtccggga tcctgcgaca gcgggcagac gtcgacgagg tccgccacgg
     4141 tgtcgccgtc gctgtcgaga cccggagcgc cgcaggcgac ggtgggggcg cgcggcgcgc
     4201 ctgtgctgtc gcttccgagg ctcccctccc cgcagaagtt ggcacccgcc tgcacgtaga
     4261 cgaacaccgc gccgagcggg ggcgcctccg cgtcgtcgaa ctgccggccc ggctgctgcg
     4321 ccgccgcgca cgagaacccg ggggacgctc cgagactggt ccgcgtggcg cggtagacgt
     4381 tgtagacgtt cgtctgcggc acgtgccccc agcgcagccg cgccaccccg ggcgccggga
     4441 tctccgccga ctccagaacc tcggccggga ggttcgacgc caggtagctc agcggcaggc
     4501 agtcctcgga atcctgcgtc ccgtcggcgt cgtcatccgg atcgacgcag tcgcgcaccc
     4561 cgtcgccgtc ggtgtcgggg aacgctcctt cgtcgatctg cgtgtcgcag tcgtcgtcga
     4621 tgccgttgca gacttccggg gcccccgggt ggatggcggc gttgaggttg ttgcagtccc
     4681 cggcgcacgt cgtcacgccg tcgtagtcct ggtcgaaccc ttcgtccacc gacgcgtcgc
     4741 agtcgtcgtc gacgagattg caggtctccg tcgcccccgg atagatcgac gggttggcgt
     4801 cgttgcaatc gttggtcgag ggaaagccgt cctggtcggc gtccgtgccg gcggcggtcc
     4861 cgagcagctt gagatcgtac gagccgtaca ggccgaaccc gaggccgtgc gcggatcgca
     4921 cgtacaggcg cgcgctcgac ggcggcgtgt aattcacgag ggaggacttg tccgacgggc
     4981 cgcggtcgtc gttcgacgcg agcaccgtca cgccgtcggt ggcgaggagc gtcagggtgg
     5041 tgttg
//
LOCUS       DASRIQ010000456         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1743477, whole genome shotgun sequence.
ACCESSION   DASRIQ010000456 DASRIQ010000000
VERSION     DASRIQ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743477"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1247
                     /locus_tag="VFD06_11130"
     CDS             54..1247
                     /locus_tag="VFD06_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN04123.1"
                     /translation="MTRRAVWALAVAAGAVLLLPWDRIDPAVSRLFYRPDVPLGPWQT
                     PQLIPWGVVAHLTPVIAIGLALTGVVALVRRPARPAIVLLLTLTLGPGLLVNVILKDH
                     WHRPRPRQTLGIGGDFAYVAPLRIGPVGKSFPCGHSAVGYACVALAGLVEARRRRIRR
                     AVVAGALALGTLLGLGRVATGAHFASDVVWSALLTFATLLVVDRAVLRQPVRAASPRR
                     PGRAVAAAAAVAAAAVASALLCFPFRHEAAAHELPIRDPGATLDLRIDVGHARLIFVP
                     PGEAALTVESRHNGFGVPWGRIDETMSAPGEEAGSPARGSGDVVRYRLASRGWFADLE
                     GTTILRLPAGAVGTVEIDVADGHLTLNDESGGAALPVIRARIRQDALRLRGNLRRDQL
                     ELVER"
     gene            complement(1223..1912)
                     /locus_tag="VFD06_11135"
     CDS             complement(1223..1912)
                     /locus_tag="VFD06_11135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN04124.1"
                     /translation="MATTRSGSARTRSRETGTIAIRPLTPARWTDLVRLFGERGACAG
                     CWCMFPRLPRSEYVRGQGALNRRRLQTLVRRGGIHGLIAYVDREPAAWCSFGPRVSFS
                     TLGRSRILAPVDDRPVWSVVCFFIRRPFRRRGLSSRLLEAAAVYAARRGAPALEGYPH
                     DPDRGPLPDAFAWNGLLASFRRAGFEEVARRSVGRPIVRRSLARARTGTAGGAARNGV
                     RPRRQRSTSSS"
BASE COUNT          246 a          777 c          806 g          309 t
ORIGIN      
        1 atcatcctca tcgggctcta cgtcctggtg cgcaacctca acgccgtggt cgcgtgaccc
       61 gccgcgcggt gtgggccctc gcggtggccg cgggggcggt actcctcctc ccgtgggacc
      121 ggatcgatcc ggcggtctcg cggctcttct accgtcccga tgtcccgctt ggtccgtggc
      181 agacgccgca gctgattccg tggggagtcg tggcgcacct gacgccggtg atcgcgatcg
      241 ggctggcgct gaccggggtc gtcgcgctcg tccggcgtcc ggcgcgaccg gcgatcgtgc
      301 tgctcctgac gctgaccctg gggccgggac tcctggtgaa cgtcatcctc aaggatcact
      361 ggcaccggcc gaggccgcgc cagacgctcg ggatcggggg agacttcgcc tacgtcgcgc
      421 cgctgcggat cggtccggtg gggaagtcgt tcccgtgcgg tcactcggcg gtcggctacg
      481 cctgcgtcgc gctcgccggg ctcgtcgagg cgcggcgccg gaggatccgg cgcgcggtcg
      541 tcgcgggggc gctcgccctc ggcacgctcc tggggctggg gcgggtcgcg accggcgccc
      601 acttcgcctc ggacgtggtc tggagcgccc tgctcacgtt cgcgacgctg ctcgtcgtcg
      661 atcgcgccgt gctccggcag ccggttcgcg ccgcgagccc aaggaggccg ggacgcgccg
      721 tcgccgccgc ggcggccgtc gcggccgcgg cggtcgcgag cgcgcttctc tgcttcccgt
      781 tccgccacga ggccgcggcg cacgagctgc cgattcggga tcccggcgcg acactcgatc
      841 tccggatcga cgtcggccac gcccggctga tcttcgttcc cccgggagag gcggcgctga
      901 cggtcgagtc gcgccacaac ggcttcggag ttccctgggg acgaatcgac gaaacgatga
      961 gcgctccggg ggaggaggcc gggtctccgg ctcgcggctc aggggacgtg gtgcgctacc
     1021 gcctggcgtc gcgcggctgg ttcgccgatc tcgaaggcac gaccatcctg aggctcccgg
     1081 cgggcgcggt cggaacggtg gagatcgacg tcgcggacgg gcacctcacg ctcaacgacg
     1141 agagcggcgg ggcggcgctc ccggtgatcc gtgcccgcat ccggcaggac gcgctcaggc
     1201 tgcgggggaa cctgcgccgc gatcagctgg aactcgtcga acgctgacgt cgcggccgca
     1261 cgccgttccg cgcggcgcct ccggccgtgc cggtgcgggc ccgcgccagg ctccggcgga
     1321 cgatcgggcg gccgacggag cgccgcgcca cctcctcgaa gccggcccgg cggaacgacg
     1381 cgagcaggcc gttccaggcg aaggcgtccg gcagcggccc ccgatcggga tcgtggggat
     1441 atccctcgag cgccggcgcc ccgcgccggg ccgcgtagac cgccgccgct tccaggaggc
     1501 gggacgacag gccgcgtcgc cggaacggtc gcctgatgaa gaagcagacg accgaccaga
     1561 ccggccggtc gtcaacgggg gccaggatgc gcgaccggcc gagggtcgag aaggaaacgc
     1621 gcggcccgaa ggagcaccag gcggccggct ccctgtccac gtaggcgatg aggccgtgga
     1681 tccctccgcg gcgcacgagc gtctgcaacc ggcgccggtt gagcgccccc tgtccgcgga
     1741 cgtactcgct ccgcggcagt cgcggaaaca tgcaccagca cccggcgcag gcgccgcgct
     1801 cgccgaacag ccgcacgagg tcggtccagc gcgccggcgt cagcggtcgg atggcgatcg
     1861 tcccagtctc cctgcttctc gtcctggctg agccgcttct cgtcgtggcc atgcagccgc
     1921 gattgtgcca tcgaccgtcc tgctgcgcgc ttgcatcccg gcggccgtct caataggctc
     1981 tgcgccatgc gcgtcggccg gttcttccgg gtgctgctgc gggtcgtcgc cgggatcgcg
     2041 ctcgtcgtcc tcgtcgtcgt ggcgggggcc gggctgtacg gatggtggat gctgcgtggc
     2101 agcctgccgg cgctcgacgg cgagcggccg ctcgcggg
//
LOCUS       DASRIQ010000457         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3855638, whole genome shotgun sequence.
ACCESSION   DASRIQ010000457 DASRIQ010000000
VERSION     DASRIQ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3855638"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFD06_11140"
     CDS             <1..673
                     /locus_tag="VFD06_11140"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZN04125.1"
                     /translation="VPFAARRAAAALWEAKWELLLPLLVLAGLFGGFMTPVESAAMTA
                     LYAAIVQCFVYRDVAPVRQLPRVMADCASLVGGFLIILCMALGLTNFLIDAEVPARIL
                     AAMQGAIRSPLIFLLILNLFLLVVGAIMDIYSALFVVVPLILPLGAAYGIDPAHLGMI
                     FLANMELGYLTPPMGENLFLSSYRFGRPVFRVFRDTWPFYVILMAGVLLITYVPALSL
                     ILLGR"
     gene            complement(684..995)
                     /locus_tag="VFD06_11145"
     CDS             complement(684..995)
                     /locus_tag="VFD06_11145"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN04126.1"
                     /translation="MPITARCGKAAEIPAGGTKVVQVRDHAIALFNMGGSFYALDNTC
                     PHQGGPLGEGYLEANGVVSCPWHGWTFDLKTGVSPIDGDVRVACYPVRVVDGEILVDL
                     P"
     gene            complement(995..1375)
                     /locus_tag="VFD06_11150"
     CDS             complement(995..1375)
                     /locus_tag="VFD06_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04127.1"
                     /translation="MPHILVEGPIALADVARRHVPWSDRRGDLLVKFDRLYLDTGGRA
                     ALVEALVVDRGHPQRFFVTLQARDGGVMVRCEPLTDPEKTAGVKRALALVAERLRSES
                     GGRYGTTNIAEFLVGAPGEDRREG"
     gene            complement(1400..1885)
                     /gene="bcp"
                     /locus_tag="VFD06_11155"
     CDS             complement(1400..1885)
                     /gene="bcp"
                     /locus_tag="VFD06_11155"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HZN04128.1"
                     /translation="MVKKTALEVGRKAPAFALLATGGREVSLSDYLGKKNLVLYFYPK
                     DGTPGCTREACGFRDIYDRLASHDTEVLGVSRDGLDAHEKFSARNGLPFPLLSDADSK
                     VATAYGVYGKKTFMGREFMGVKRTTFVIDKKGKIRRIDNPVEVQKHPDEILDYVRTVL
                     A"
     gene            complement(1984..2139)
                     /locus_tag="VFD06_11160"
     CDS             complement(1984..2139)
                     /locus_tag="VFD06_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04129.1"
                     /translation="MSYHLLNAVGAALLLVVAVAARQSGFIVLEGVWTAISVVALWRS
                     LQSPRAS"
     gene            complement(2226..>2872)
                     /locus_tag="VFD06_11165"
     CDS             complement(2226..>2872)
                     /locus_tag="VFD06_11165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN04130.1"
                     /translation="SALGSPETSAAFLKEIGATEADLVAQVRRTISIGRFVDQKVAGS
                     LTVGEKEARAWYDTHPEAMTRPESAHIRQIVVQVTPDASPAARAAARQKVEEILKSLR
                     GGEDFAALARRYSDGPEAARGGDGGWIWAGSSALPAVERAAMTLKPGQTSDILESRRG
                     FHIVQVTERRPAGTVPFDEARERIIARLKDEQRQTRVKEYVATLRASARIEKLA"
BASE COUNT          384 a         1022 c          978 g          488 t
ORIGIN      
        1 ggtgccgttc gcggcgcgcc gggcggcggc ggcgctgtgg gaggcgaagt gggagctgct
       61 gctgccgctc ctcgtcctgg cgggactctt cggcgggttc atgactccgg tggagtcggc
      121 ggcgatgacc gcgctctacg cggcgatcgt gcagtgcttc gtgtaccgcg acgtcgcgcc
      181 ggtgcggcag ctgccgcgcg tcatggccga ctgcgcctcg ctcgtcggcg ggttcctcat
      241 catcctgtgc atggcgctcg ggctgaccaa cttcctcatc gacgccgaag tgccggcccg
      301 gatccttgcg gcgatgcagg gggcgatccg gagtccgttg atcttcctgc tgatcctcaa
      361 cctgttcctg ctcgtggtcg gggcgatcat ggacatctac tcggcgctct tcgtggtcgt
      421 gccgctcatc ctgccgctcg gcgccgcgta cggcatcgac ccggcgcacc tcgggatgat
      481 cttcctcgcc aacatggaac tcggctacct gacgccgccg atgggggaga acctgttcct
      541 gtcgtcgtac cgtttcggcc gtccggtctt ccgggtgttc cgcgacacct ggccgttcta
      601 cgtcatcctc atggcggggg tgctgctcat cacctacgtc ccggccctgt cactcatcct
      661 gctcgggcgc tgaaaagact tctctagggc aggtcgacga ggatctcgcc gtcgacgacg
      721 cgcaccgggt agcaggcgac gcggacgtcg ccgtcgatcg gcgacacgcc ggtcttcaag
      781 tcgaaggtcc agccgtgcca ggggcacgac acgacgccgt tcgcttcgag atatccctcg
      841 cccaggggtc ctccctgatg cgggcaggtg ttgtcgagag cgtagaacga gccgcccatg
      901 ttgaacaggg cgatggcgtg gtcgcgaacc tgcaccacct tcgtgcctcc ggccgggatc
      961 tccgccgcct tgccgcaccg cgccgtgatc ggcatcagcc ttccctccgg tcttcgccgg
     1021 gcgccccgac gaggaactcc gcgatgttgg tggtgccgta gcgccccccc gactcggagc
     1081 gcagacgctc ggcgacgagg gccagcgccc gcttcacgcc cgccgtcttc tccgggtcgg
     1141 tgagcggttc gcagcgcacc atgaccccgc cgtcccgcgc ctgcagcgtc acgaagaagc
     1201 gctgcggatg gccgcgatcg acgaccagcg cctcaacgag cgcggcgcgg ccgccggtgt
     1261 cgaggtacag gcggtcgaac ttcacgagga gatcgccccg gcgatcgctc cacggcacat
     1321 gccggcgcgc cacgtcggcg agcgcgatcg ggccttcgac caggatgtgg ggcaccgcgg
     1381 cgtcgggacc ggaatggtcc tatgcgagga cggtgcggac gtagtcgagg atctcgtccg
     1441 ggtgcttctg tacctcgacc gggttatcga tgcggcggat cttccccttc ttgtcgatca
     1501 cgaaggtggt ccgcttgacg cccatgaact cgcgccccat gaaggtcttc ttgccgtaca
     1561 cgccgtaggc ggtcgcgacc ttggagtcgg cgtcggagag gagcgggaac ggcaggccgt
     1621 tgcgcgccga gaacttctcg tgggcgtcga ggccgtcgcg gctgacgccg agcacctcgg
     1681 tgtcgtgcga cgccagccga tcgtagatgt cgcggaaccc gcaggcctcc cgggtgcatc
     1741 ccggcgtccc gtccttgggg tagaagtaca ggacgaggtt cttcttcccc aggtagtcgc
     1801 tcagcgacac ttcgcgccct ccggtcgcca ggagcgcgaa cgccggagcc ttgcgaccga
     1861 cctccagagc cgtcttcttc accatcggca cactctccct cgcccggccg cgcgttcgct
     1921 ctgccatggg cgcgtctcgt tgtcgcgggg tcatcattct agcatgccgc accgggcgat
     1981 tcgtcaggag gcgcgcgggc tttggaggct ccgccagagc gcgaccacgc tgatcgccgt
     2041 ccacaccccc tcgagcacga tgaacccgct ctggcgcgcc gccaccgcga ccacgagcag
     2101 gagggccgcc ccgaccgcgt tgagcagatg ataggacacc acgtcgcgcc cgagccgtcc
     2161 cgcctggtgg gcggcgtacg cgatcaggat caggaacgcg ccgaggaggg acgtcgcctg
     2221 caggatcagg ccagcttctc gatccgcgcc gaggcgcgca gcgtcgcgac gtactccttg
     2281 acgcgcgtct ggcgctgctc gtccttgagc cgcgcgatga tgcgttcgcg cgcctcgtcg
     2341 aacggcacgg tcccggccgg tcgacgctcg gtcacctgga cgatgtgaaa gccgcggcgg
     2401 ctctcgagga tgtcactcgt ctgccccggc ttgagcgtca tcgcggcccg ctccaccgca
     2461 gggagcgcgc tgctgccggc ccagatccag ccgccgtcgc cgccgcgcgc cgcctccgga
     2521 ccgtcggagt aacggcgcgc cagcgcggcg aagtcctcgc cgccgcgcag cgacttcagg
     2581 atctcctcga ccttctgccg cgccgccgca cgcgccgcgg gggaggcgtc cggggtgacc
     2641 tgcacgacga tctggcggat gtgcgccgac tcggggcgcg tcatcgcctc agggtgcgtg
     2701 tcgtaccagg cgcgcgcctc cttctcgccg acggtgagcg aaccggcgac cttctggtcg
     2761 acgaatcggc cgatgctgat cgtccggcgc acctgcgcca ccaggtccgc ctcggtggcg
     2821 ccgatctcct tcaggaaggc ggcggaggtc tcgggggagc cgagggcgct ct
//
LOCUS       DASRIQ010000458         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3292412, whole genome shotgun sequence.
ACCESSION   DASRIQ010000458 DASRIQ010000000
VERSION     DASRIQ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3292412"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /gene="rpsM"
                     /locus_tag="VFD06_11170"
     CDS             complement(<1..103)
                     /gene="rpsM"
                     /locus_tag="VFD06_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZN04131.1"
                     /translation="MARIAGVDLPAEKRIEVALTYIFGIGRTTARRIL"
     gene            complement(173..289)
                     /gene="rpmJ"
                     /locus_tag="VFD06_11175"
     CDS             complement(173..289)
                     /gene="rpmJ"
                     /locus_tag="VFD06_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZN04132.1"
                     /translation="MKVRSSVKKICENCKIVRRKGVIRVICTRNPRHKQRQK"
     gene            complement(324..542)
                     /gene="infA"
                     /locus_tag="VFD06_11180"
     CDS             complement(324..542)
                     /gene="infA"
                     /locus_tag="VFD06_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZN04133.1"
                     /translation="MAKEEGIQVEGTVVEPLPNAMFRVELENGHKVLAHISGKMRMHF
                     IKILPGDKVTVELSPYDLTRGRIVYRYK"
     gene            complement(554..1318)
                     /gene="map"
                     /locus_tag="VFD06_11185"
     CDS             complement(554..1318)
                     /gene="map"
                     /locus_tag="VFD06_11185"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZN04134.1"
                     /translation="MARTVFLRGKEEIDAIRAAARLVAQTLEMLGPEVRPGVTTADLD
                     RMAETFIRERGARPAFKGYRGFPASICPSINDEVVHGIPGSRALAEGDIVGVDVGVEM
                     GGYYGDAAFTFPVGRVSDEATRLLRVTQEALMRGVAQAKAGNRVGDISHAIQSYVEAQ
                     GYSVVRSLVGHGIGRHMHEEPQVPNYGTPDRGPRLMAGQVLAIEPMVNAGAPEVLTQP
                     DGWTVVTKDGSLSAHFEHTVAVGPDGPEILSRADGS"
     gene            complement(1328..1975)
                     /locus_tag="VFD06_11190"
     CDS             complement(1328..1975)
                     /locus_tag="VFD06_11190"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZN04135.1"
                     /translation="MRVVLLGPPGVGKGTQGRRLASGHGWALISTGEMLREAIAKRTP
                     LGIEAGRLVDAGQLVGDDVMIALVKERSGQSDAAAGFVMDGYPRTVPQAEALDAMLSE
                     RGQTLDAAVSLAADEGELVRRLSARRECPICKRAYNLVSAPPRDGRHCDDHPAAELVR
                     RADDAPETVTRRLDVYQRATAPLLEHYRSRSRLREVPGLGTMDEVYGSLTRSLGI"
     gene            complement(1976..>2166)
                     /locus_tag="VFD06_11195"
     CDS             complement(1976..>2166)
                     /locus_tag="VFD06_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZN04136.1"
                     /translation="IATIPDMLIASMGLPFLGFGGTSVLIVVGVALDTLQQVESHLLL
                     RHYEGFVKRGRIRGRQRM"
BASE COUNT          344 a          834 c          617 g          371 t
ORIGIN      
        1 cgagaatcct gcgtgcggtg gtgcgaccga tgccgaagat gtaggtgagc gcgacctcga
       61 tgcgcttttc cgccggaaga tcgacgcccg cgatacgtgc cacggatacc ctccttcgct
      121 ggactcggcc tgagaacccc gattgctgga ctcgggcctc tcgggccggg tcctatttct
      181 gacgctgctt gtgccgcgga ttgcgcgtgc agatcacgcg gatcacgcct tttcgacgca
      241 cgatcttgca gttctcacag atcttcttga ccgaagatcg gaccttcatg ccacacctcc
      301 ctgctcctcg gagccccggg cgcctacttg tatcgataca cgatgcgccc gcgcgtcaga
      361 tcgtagggcg agagctccac cgtgactttg tctcccggaa ggatcttgat gaaatgcatc
      421 cgcatcttgc ccgagatgtg ggcgagcacc ttgtggccgt tctccagctc caccctgaac
      481 atcgcgttgg ggagcggctc gaccacggtg ccctcgacct ggatgccttc ttctttcgcc
      541 aatacctttt ctcctatgac ccgtccgccc gcgagagaat ctcggggcca tccgggccca
      601 ccgccacggt gtgctcgaag tgggcactca gggagccatc cttcgtcacc accgtccagc
      661 cgtccggctg ggtcaggacc tccggcgcac cggcattcac catcggctcg atcgccagca
      721 cctgccccgc catgagccgc ggaccccgat ccggcgtgcc gtaattcggg acctgcggct
      781 cctcgtgcat gtggcggcca atcccatgtc cgaccagcga cctcaccacc gagtacccct
      841 gcgcctcgac gtaggactgg atcgcgtggg agatgtcccc cacccggttc cccgccttcg
      901 cctgagccac acctcgcatc aacgcctctt gcgtcacccg cagcagccgg gtcgcttcat
      961 cgctcacacg gcccaccgga aaggtgaagg ccgcatcgcc gtaatagccc cccatctcga
     1021 ccccgacatc caccccgacg atatcgccct cggccaacgc ccggcttccc gggatcccgt
     1081 ggaccacctc gtcgttgatc gacggacaga tgctggcggg aaacccgcgg tagcccttga
     1141 acgcgggccg cgcgccccgc tcacggatga acgtctccgc catccgatcc aggtccgccg
     1201 tcgtcacgcc cggccgcact tccgggccga gcatttcgag cgtctgcgcc accagcctgg
     1261 ctgccgcccg aatcgcgtcg atctcctcct tgcccctcag gaacaccgtt cgcgccacgc
     1321 gggccaccta gatcccgagc gaccgcgtca acgacccgta gacctcgtcc atcgttccca
     1381 gccccggcac ttcccggagc ctgctccgcg agcggtagtg ctcgagcagc ggcgccgtgg
     1441 cgcgctgata gacatcgagc cgccgggtca ccgtttccgg cgcgtcatcc gcccgccgca
     1501 ccagctccgc ggcgggatgg tcgtcgcaat gacgcccgtc ccgcggcggc gcactgacca
     1561 ggttgtacgc ccgcttgcag atcgggcatt cccggcgcgc gctcaggcgc cggaccagct
     1621 ctccttcgtc ggcggccaga ctcaccgccg catccagcgt ctgaccgcgc tcactcaaca
     1681 tcgcatccaa cgcctcggcc tgcgggacgg tgcgagggta tccgtccatc acgaaccccg
     1741 ccgccgcgtc cgactgtccg ctgcgttcct tcaccagtgc gatcatcacg tcgtcaccga
     1801 ccagctgtcc ggcatccacc agccgcccgg cctcgatccc cagcggcgtc cgcttcgcga
     1861 tcgcctcgcg cagcatctcg ccggtcgaga tcaacgccca gccatgtccg gaagcgagcc
     1921 gccgaccctg ggttcccttt ccgaccccgg gcggccccag caggacaacc ctcactcaca
     1981 tcctctggcg gccacgaatg cggccgcgct tcacgaatcc ctcgtagtgc ctcagcagga
     2041 gatgcgactc gacctgctgc agggtgtcga gcgccacgcc cacgacgatg agcacgctgg
     2101 tgccgccgaa gccgaggaaa ggcaggccca tcgaagcgat gagcatgtcc gggatggtgg
     2161 cgatga
//
LOCUS       DASRIQ010000459         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1500106, whole genome shotgun sequence.
ACCESSION   DASRIQ010000459 DASRIQ010000000
VERSION     DASRIQ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500106"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /gene="atpH"
                     /locus_tag="VFD06_11200"
     CDS             <1..525
                     /gene="atpH"
                     /locus_tag="VFD06_11200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HZN04137.1"
                     /translation="RYARALAEIAGEKDAQALAALAVEVDLLAATIGTDAALVRFFDS
                     PTMGDREKQAAIATLAEQARLSDIARRFLTVVVEHRRTGALRAIAHDLAAFRDRAAGI
                     VEAEATFAKDPGPAEEQAMQAALETMTGSRVRLSVKVDPDLLGGASARVGSRIYDGSL
                     RTRLQALHRRLARA"
     gene            586..2271
                     /gene="atpA"
                     /locus_tag="VFD06_11205"
     CDS             586..2271
                     /gene="atpA"
                     /locus_tag="VFD06_11205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZN04138.1"
                     /translation="MAQIKADEIVSVLKQQIEGFKGGVDVSEVGTILSVGDGIARIHG
                     LDRCMAGELLELPHGVFGLALNLEEGSVGAVLLGEFMKIKEGDTVKRTGRIVEVPVGK
                     GLVGRVVDPLGQPQDGKGPVATSETGHLERIAPGVVDRQPVKEPLQTGIKAIDAMIPI
                     GRGQRELIIGDRQTGKTAIAVDTILNQKGNGVICVYVAIGQKRSTIAQVVRTLDDQKA
                     MDYTIVVAASASEPATMQYIAPYAGCAMGEYFRDNKQHALVIYDDLSKHAAAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLNDKLGGGSLTALPMIETQAGDVSAYIPTN
                     VISITDGQIYLESDLFFSGVRPAVNVGLSVSRVGGNAQTKAMKSVAGTLRLDLAQYRS
                     MAAFAQFGSDLDKATQSQLHRGKRLTELLKQGQYQPIPVEKQIIALAAGVSGLLDDLE
                     VEQIRPFEEALYRHLDGPGRELARQVVEKKAIDDALKAEIRKTVEEVKATFKATQPGA
                     AAGTAKPPADDSKGGSTPKQGGASGSGAAGAKPAAASRPAEKKPEPAAAGAAH"
     gene            2298..>3160
                     /gene="atpG"
                     /locus_tag="VFD06_11210"
     CDS             2298..>3160
                     /gene="atpG"
                     /locus_tag="VFD06_11210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HZN04139.1"
                     /translation="MANVRDFRTRIRSVKSTQQITRAMYLVAAAKMRRAQERIVAVRP
                     YALKMLDVLSSLAARADAAKHPLLARREERRVIVLVISSDKGLCGSFNTNIMNRARLF
                     LDELRDRDVQVDIVGRKARDWFKRRAYTVRRVEVDLLKVLDVPKVREFALELVRGFTA
                     QEYDAVYMIYNEFKSILQQRPVVEKLLPIPLEIFERRKAEGGGGETKPGAPAGASATA
                     TEDYIYEPDDRSLFDRLLPRHVGTQVYRALLESGAAEQGARMTAMNNATSNASDMIDA
                     LTLQMNKIRQAS"
BASE COUNT          523 a         1076 c         1159 g          402 t
ORIGIN      
        1 cgctacgccc gcgccctggc cgagatcgcc ggggagaagg acgcgcaggc gctcgcggcg
       61 ctcgcggtgg aggtcgatct cctcgccgcg accatcggca cggacgccgc gctcgtgcgc
      121 ttcttcgaca gcccgacgat gggcgatcgg gagaagcagg cggcgatcgc cacgctggcg
      181 gagcaggcgc gcctctcgga catcgcccgc cgcttcctca cggtcgtcgt cgagcaccgc
      241 cgcaccggcg cgctccgggc gatcgcccac gatctggcgg cgttccgcga ccgggccgcc
      301 ggcatcgtgg aggcggaggc gaccttcgcg aaggaccccg ggccggccga ggagcaggcg
      361 atgcaggcgg cgctcgagac gatgaccggg agccgggtgc ggctgtcggt gaaggtggac
      421 ccggaccttc tgggcggcgc ttcggcgcgg gtcgggtcgc gcatctacga cggatcgctc
      481 aggacgcggc tccaggcgct gcaccgtcgc ctggcgcggg cctgaaccgg gcgcggcgac
      541 gcaccggcca cgggacacga acggcacagg gagcagggag acgacatggc gcagatcaag
      601 gcggacgaga tcgtcagcgt cctgaagcag cagatcgagg ggttcaaggg gggcgtcgac
      661 gtcagcgagg tcggcaccat cctgtcggtc ggcgacggca tcgcccgcat ccacggcctc
      721 gaccgctgca tggccggcga gctcctggag ctgccgcacg gcgtgttcgg cctggcgctg
      781 aacctcgagg aggggtcggt cggcgcggtg ctcctcggcg agttcatgaa gatcaaggaa
      841 ggggacacgg tgaagcgcac cggccgcatc gtcgaggtgc cggtcgggaa ggggctcgtc
      901 ggccgcgtcg tcgatcccct cggccagcct caggatggca agggcccggt cgcgaccagc
      961 gagaccggcc acctcgagcg catcgccccc ggcgtcgtgg accggcagcc ggtgaaggaa
     1021 ccgctgcaga ccggcatcaa ggcgatcgac gcgatgatcc cgatcggccg cggccagcgc
     1081 gagctgatca tcggcgaccg ccagaccggc aagacggcga tcgcggtgga caccatcctc
     1141 aaccagaagg ggaacggcgt catctgcgtc tacgtcgcca tcggtcagaa gcgctccacc
     1201 atcgcccagg tggtgaggac gctcgacgat cagaaggcga tggactacac catcgtcgtc
     1261 gcggcgtcgg cgtcggagcc ggcgaccatg cagtacatcg cgccgtacgc cggctgcgcc
     1321 atgggggagt acttccgcga caacaagcag cacgcgctgg tcatctacga cgacttgtcg
     1381 aagcacgccg ccgcctaccg cgagatctcg ctgctgctgc gccggccgcc gggacgcgag
     1441 gcgtacccgg gggacgtctt ctacctgcac tcgcgcctgc tggagcgggc ggcgaagctg
     1501 aacgacaagc tcggcggcgg atcgctgacg gcgctgccga tgatcgagac gcaggcgggt
     1561 gacgtgtcgg cctacatccc gacgaacgtg atctcgatca ccgacgggca gatctacctc
     1621 gagtcggatc tgttcttctc cggcgtgcgg ccggcggtca acgtcgggct gtcggtcagc
     1681 cgcgtcggcg gcaacgccca gaccaaggcg atgaagtcgg tcgccgggac cctgcgcctc
     1741 gacctcgcgc agtaccgctc gatggcggcg ttcgcgcagt tcgggtccga cctcgacaag
     1801 gcgacccaat cgcagctgca ccgcggcaag cggctcaccg agctgctcaa gcaggggcag
     1861 taccagccga tcccggtcga gaagcagatc atcgcgctcg ctgccggcgt ctcgggactc
     1921 ctcgacgatc tcgaggtgga gcagatccgg ccgttcgagg aggcgctgta ccgccatctc
     1981 gacgggccgg gccgggagct ggcgcgccag gtggtcgaga agaaggcgat cgacgacgcc
     2041 ctcaaggccg agatccggaa gacggtcgag gaggtgaagg cgaccttcaa ggccacgcag
     2101 cccggcgcgg ccgccgggac tgcgaagccg ccggcggacg actcgaaggg cgggtcgacg
     2161 ccgaagcagg ggggcgcgtc ggggtccgga gccgccggcg cgaagccggc cgcggcctcc
     2221 cggccggccg agaagaagcc ggagccggcg gcggcgggcg ccgcgcactg acgcgcccgg
     2281 gatcctgaga gcgccgcatg gccaacgtcc gcgacttccg cacccgcatc cgcagcgtca
     2341 agagcacgca gcagatcacc cgcgccatgt acctcgtggc ggcggcgaag atgcggcgcg
     2401 cccaggagcg gatcgtcgcg gtccgtccct acgccctgaa gatgctcgac gtcctctcgt
     2461 cgctggcggc gcgggccgac gccgcgaagc acccgctcct ggcgcggcgc gaggagcgcc
     2521 gggtgatcgt gctggtcatc tcctccgaca aggggctgtg cggctcgttc aacaccaaca
     2581 tcatgaaccg ggcgcgcctc ttcctcgacg agctgcgcga ccgtgacgtg caggtggaca
     2641 tcgttggccg caaggcgcgc gactggttca agcggcgcgc ctacacggtg cggcgcgtcg
     2701 aggtcgatct cctgaaagtg ctcgacgtcc cgaaagtgcg ggagttcgcc ctggagctgg
     2761 tgcggggctt cacggcgcag gagtacgacg cggtctacat gatctacaac gagttcaagt
     2821 cgatcctgca gcagcgtccg gtcgtcgaga agctcctgcc gatcccgctc gagatcttcg
     2881 agcgacggaa ggcggagggg ggcggcggcg agacgaagcc cggcgccccg gccggcgcct
     2941 cggcgacggc gaccgaggac tacatctacg agccggacga ccggtcgctc ttcgaccgcc
     3001 tgctgccgcg ccacgtcggc acgcaggtct accgggcgct cctggagtcg ggggcggcgg
     3061 agcagggggc gcgcatgacg gcgatgaaca acgccacgag caacgcgtcg gacatgatcg
     3121 acgcgctgac gctgcagatg aacaagatcc gccaggcctc
//
LOCUS       DASRIQ010000460         8400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2801351, whole genome shotgun sequence.
ACCESSION   DASRIQ010000460 DASRIQ010000000
VERSION     DASRIQ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2801351"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFD06_11215"
     CDS             complement(<1..303)
                     /locus_tag="VFD06_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HZN04140.1"
                     /translation="MADEPAPQGTSKPVVLVILGTRPEAIKLFPVARALQREPRLVTR
                     VVTTGQHREMVDQILGPMGIVPDRDLDIMRPDQTLNDIVSRIMPRLEALYDEERPAL"
     gene            complement(296..1273)
                     /locus_tag="VFD06_11220"
     CDS             complement(296..1273)
                     /locus_tag="VFD06_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN04141.1"
                     /translation="MKPLLSLGLDLDNLWSYMKIHGDAGWESHPTYLEPLAELLEEFT
                     RRHQIRLTIFIVGQDAALAKNAQAFARLAALGHEMGNHSFKHEPWFHLYARPEIEDEI
                     ARTEDAIERATGQRPKGFRGPGYSLSPDTLRVLAARNYLYDCSTLPTFLGPLARAYYF
                     WKSRGMPQAEREKRSKLFGRLRDGLQPIRPYAWQVEGRRLVELPVTTMPIVRVPFHMS
                     YLLYLSRFSQAAAWAHFRSSLTLCRWGGITPSFLLHPLDFLGCDKVKELAFFPGMDLP
                     TSLKIEFVDAGMERLKKMFEVVPLEEAARRVPTAAMAPVRTLNAEGAFG"
     gene            complement(1270..3054)
                     /locus_tag="VFD06_11225"
     CDS             complement(1270..3054)
                     /locus_tag="VFD06_11225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN04142.1"
                     /translation="MASMGEVRKHVLTVSVEDYFHGASLDGVVARKHWTRIESRLEKS
                     LKEVVDLLAEHQATATFFVLGWIAERQPEIVRLIRAAGHEIASRGYWPRGGDKGMLPH
                     PQELREELQRARQALEAAGAHRILGYRHPRWIMRPEELWVIDVLVEEGYLYDSSVNPI
                     FRRFARDPRWFEAHQHRHSVTNQSIWVFPASTASVLGERVTISGGNYVRQLPHSLLRR
                     AVRSWDRHRPTPIVFYFMPWEMDRDQPQIRGISRLQRIRQYRSLGKTRDVFQEYLKEY
                     RFQPIGDWLGLPWRTTEPQERAAPPARLEPAISLRSDPEDGATVAAADRQPVTLVVPL
                     YNEEANIPYMRGTLLDLRRRLERRYRIHFNLVDDGSSDATWERLSERFRDFPDCQVLR
                     HPKNAGVAAAILTGLRHAPTEIVASIDCDCSYDPNDLEGMIPMIAEADLVTASPYHPQ
                     GQVYNIPPWRLFLSQSLSWMYSLLLGKSIHTYTSCFRVYRRSAVEGLRVEHGGFLGVA
                     EILIRLRLQGGRIVEYPNTLESRLFGESKMKIARTIWSHLGLLRELAILKVRGRTGEP
                     AAASGAATRTAPSARTVRKESTAPGVEK"
     gene            complement(3189..4187)
                     /locus_tag="VFD06_11230"
     CDS             complement(3189..4187)
                     /locus_tag="VFD06_11230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN04143.1"
                     /translation="MADPCPVEPPAPRSPEPPEARPADPRTPRPSYAEPATHGEPATR
                     ASEAPVTRSSGPPPCPVCRGTAWSARRSYTALRNCGDCGTVLNDRSSSRTEEEARYRD
                     TGLPLEGIDQTIAARRWRFTVRWAGGTPHRVLDVGCGAGAFLEAAHGDGAEVAGIEID
                     PAAANAARSRGVSVTIGSILSPVPLGYGWDVVTLWDVLDHLDDPAEALARLRPRMKPG
                     GLLVARGRNATLHAPLKRLTLLLRPWLPFVPDPAVVHRFGLRPEGWARLLAQAGFEQV
                     RWHPAHIGGLLPSVLLTARAPHAAPPAAWAGAAAAAARGEAAPGAGRNVPDRSDEY"
     gene            complement(4206..5174)
                     /locus_tag="VFD06_11235"
     CDS             complement(4206..5174)
                     /locus_tag="VFD06_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN04144.1"
                     /translation="MSLAAEAPAGGGPGPEGSESARRRHALLVTLNFAAVYILWGSTY
                     LAIRAGVATLPPALMAGTRFVIAGAILFAWLMFRRVPLPSRDLWGPIALTGMLLLFGG
                     NLLVTVAEMTVESGMAAVIIANLPFVMAILEYFRKDGERLSFLGGLGLVAGFAGMLLL
                     TWPKIVASLSLGIGTMWGEAALLGANLCWAVGSIYSKHRLRNVSPLMATALQMLIAGA
                     ALCLLGLVLGEAPRFRLDAVSAAAVAWLIVAGSLVAYSAYNWLLVHVPAVKVATYAYV
                     NPVIALVLGWLLLDEPLGWRVWAGTLVILSGVAIVNLTRVRVRTAA"
     gene            5270..5998
                     /locus_tag="VFD06_11240"
     CDS             5270..5998
                     /locus_tag="VFD06_11240"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN04145.1"
                     /translation="MSEPCAYKWPALLVVMRHAQSEANFHREYLEQQESLETHVTVQA
                     RDADVRLTSEGERQAREAGRYLREEHGRFDAAYISPYRRTRDTADFAIAALDPAPVKV
                     IEERLRERDFGLIESLTRHGFKTLHPGEWERRKLVGKYYYRPPGGESYPDVNLRVHSF
                     LGTLVREHPGQRVLVVTHSVVVLAFRRLIERLGEEEVLDLDRSNEPKNASLSIYESFP
                     GETIGTGLALQQWNFVPKEAACRT"
     gene            5986..6648
                     /locus_tag="VFD06_11245"
     CDS             5986..6648
                     /locus_tag="VFD06_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZN04146.1"
                     /translation="MSDLKLFVGEKNISSWSMRAWVAITHKGVPFEEAPLPLKGDKDR
                     SRRRTVSPTGKVPVLHHKGVAIPDSLAIIEYLEETFPPPQHLALWPRDAAERAHARWL
                     AATMHSGFMGMREGMSFNTCFLKTRPAPTAAALADAAELLGLLEVPLSKRRAGSGPYL
                     FGAFGAVDAMYAPAAVRLIAYEAPAGKSPLAHAYLRDVLAAPAVEPWMDAARALPPAE
                     TY"
     gene            6649..7032
                     /locus_tag="VFD06_11250"
     CDS             6649..7032
                     /locus_tag="VFD06_11250"
                     /inference="COORDINATES: protein motif:HMM:NF016050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HZN04147.1"
                     /translation="MAEKAVRIEAGGVRLYAILSDTATASAFWEALPLEGKVRLYGQA
                     ILVDAKLGVPLEPIARAQVKAGDLAFWPDGPAVGLFFGRTPASTGDDPIAASPVNVFG
                     RITGDSGRLARVVEGAKVKITALEG"
     gene            complement(7064..8212)
                     /locus_tag="VFD06_11255"
     CDS             complement(7064..8212)
                     /locus_tag="VFD06_11255"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HZN04148.1"
                     /translation="MTESKPAGRLERLQAFRGQHVKAEIATFFAAGMLFDIVTLGRVD
                     DTFTLVQQGVYLALLVWLMILEERERAKVWAPPRLLAKAWRFSEDAIHFLLGSLLSSF
                     TLLYLKSSSGLSAFLFMSLLGGLLVANELPRFRERGPVVRWALLSLCLTSYLALLLPV
                     LIGFLSKWLFVLAVVLSCAAFAWLVRRLSKRKSAAADGQGMESDSSSLSRVASAGFGI
                     QAVLVGAYFLGAIPPVPLSLQYIGIYHEVLPPSAQPAGAITYELRHQRPWWKPWQHGD
                     QDFKARPGDVIYCFARVFAPTGFRDAIYVRWLVKGQGGWQDQGRARLDINGGRDKGFR
                     VIATKKSYQPGRWRVEVETDDGRDLGVIHFDLISDASSDVREYKTDRL"
BASE COUNT         1336 a         2882 c         3014 g         1168 t
ORIGIN      
        1 gagcgccggc cgctcctcgt cgtagagcgc ctcgaggcgc ggcatgatgc gggagacgat
       61 gtcgttgagc gtctggtcgg ggcgcatgat gtcgagatcc cggtccggga cgatgcccat
      121 cggccccagg atctgatcga ccatctcgcg gtgctgcccg gtcgtcacga cgcgcgtgac
      181 gaggcgcggc tcacgctgca gcgcgcgggc caccgggaag agcttgatcg cctcgggacg
      241 cgtgccgagg atgaccagga ccaccggctt cgacgtcccc tgcggggcgg gctcgtcagc
      301 caaaggcccc ctccgcgttg agggtgcgca ccggcgccat ggcggcggtc ggcacgcgcc
      361 gggccgcctc ctcgaggggc accacctcga acatcttctt caggcgctcc atcccggcgt
      421 ccacgaactc gatcttgagc gaggtcggca ggtccatccc ggggaagaag gccagctcct
      481 tcaccttgtc gcagccgagg aaatcgagcg ggtgcaggag gaacgacggg gtgattccgc
      541 cccagcggca cagggtgaga gaggaccgga agtgcgccca cgccgccgcc tgcgagaagc
      601 gcgacagata caggaggtac gacatgtgga agggcacccg gacgatcggc atggtggtca
      661 ccggcagctc gacgaggcgg cgaccttcca cctgccaggc gtacggccgg atcggctgca
      721 gtccgtcccg cagccggccg aacagcttgc tgcgcttctc ccgctccgcc tgcggcatgc
      781 cgcggctctt ccagaagtaa tacgcgcggg ccagcggccc gaggaaggtc gggagcgtgc
      841 tgcagtcgta gaggtagttc cgcgccgcga gcacccgcag cgtgtcgggg ctgaggctgt
      901 agcccgggcc gcgaaagccc ttcgggcgct gcccggtggc gcgctcgatg gcgtcttcgg
      961 tcctggcgat ctcgtcctcg atctccgggc gggcgtagag gtggaaccac ggctcgtgct
     1021 tgaacgagtg gttccccatc tcgtgcccga gcgccgcgag ccgcgcgaag gcctgggcgt
     1081 tcttcgcaag cgcggcgtcc tggccgacga tgaaaatggt caggcggatc tggtgccggc
     1141 gcgtgaactc ctcgagcagc tccgccagcg gctcgagata ggtcggatgc gactcccatc
     1201 ccgcgtcgcc gtgaatcttc atgtacgacc agaggttgtc gaggtcgaga ccgaggctca
     1261 ggagcggctt catttctcga ctcccggcgc cgtcgattcc ttgcggaccg tgcgggcgga
     1321 cggcgcggtc cgcgtcgcgg cgccggacgc ggcggccggc tctccggtcc ggccgcgcac
     1381 cttgagaatc gccagctcgc gcagcaggcc gaggtggctc cagatggtgc gcgcgatctt
     1441 catcttcgac tcgccgaaca ggcgcgactc gagcgtattc gggtattcga cgatgcgccc
     1501 gccctggagg cgcagccgga tgaggatctc ggcgacgccg aggaacccgc catgctcgac
     1561 gcgcaatcct tcgaccgcgc ttctccgata aaccctgaag caggacgtgt aggtgtggat
     1621 cgacttcccg agcagcaggc tgtacatcca cgacagcgac tgcgacagga acagccgcca
     1681 cggtgggatg ttgtagacct gcccctgcgg gtggtacggc gacgccgtga cgagatcggc
     1741 ctcggcgatc atcgggatca tcccctcgag gtcgttcggg tcgtaggagc agtcgcagtc
     1801 gatcgacgcg acgatttcgg tcggggcatg gcgcagcccg gtcaggatcg ccgccgccac
     1861 cccggcgttc ttcgggtggc gcagcacctg gcagtcgggg aagtcgcgga agcgctccga
     1921 cagccgctcc caggtggcgt cgctgctgcc gtcgtccacc aggttgaagt ggatgcggta
     1981 gcggcgctcc aggcggcggc gcagatcgag gagcgtgccg cgcatgtacg ggatgttcgc
     2041 ttcctcgttg tacagcggca cgacgagggt caccggctgc cggtcggccg cggcaacggt
     2101 ggccccgtcc tccggatcgc tgcgcagcga aatcgccggc tcgaggcggg cggggggagc
     2161 ggctcgctcc tgaggctcgg tcgtccgcca cggcagaccg agccagtcgc cgatcggctg
     2221 gaagcggtat tccttcaggt actcctggaa cacgtcgcgc gtcttgccga gcgaccggta
     2281 ctgccggatg cgctgcagcc tcgagatgcc ccggatctgc ggctggtccc ggtccatctc
     2341 ccacggcatg aaatagaaga cgatcggcgt gggccggtgc cggtcccagg agcgcaccgc
     2401 gcgtcggagg agactgtgcg gcagctgccg cacgtagttg ccgccggaga tcgtcacccg
     2461 ctcgccgagc acgctcgcgg tcgaggccgg gaagacccag atcgactggt tggtgaccga
     2521 gtgccggtgc tggtgcgcct cgaaccagcg cgggtcgcgc gcgaaacgcc ggaagatcgg
     2581 gttgacgctg ctgtcgtaga ggtatccctc ctccaccagc acgtcgatga cccacagctc
     2641 ctcggggcgc atgatccagc gcgggtggcg gtagccgagg atgcggtgcg cgccggccgc
     2701 ctcgagcgcc tggcgggcgc gctgcagctc ctcgcgcaat tcctggggat gcggcagcat
     2761 ccccttgtcg ccgccgcgcg gccagtagcc gcggctggcg atctcgtggc cggcggcgcg
     2821 gatgagacgg acgatctccg gctggcgctc ggcgatccag ccgaggacga agaaggtggc
     2881 ggtcgcctgg tgctcggcga ggaggtcgac cacctccttc aggctcttct cgagacggga
     2941 ctcgatgcgg gtccagtgct tgcgcgcgac gacgccgtcg agcgacgcac cgtggaagta
     3001 gtcctcgacc gagacggtca ggacgtgctt gcggacttcc cccatcgacg ccatccccct
     3061 atcccatacg agacgctccc gccacggtca aggcggaagg atcgttccag gtccccgtcg
     3121 cgaaatcgta atcgatcccg tctgatttca cgcgggtttc cggtcggacg gtcctgaggt
     3181 gcgcttcctt aatattcgtc ggaccggtcc gggacgttcc ggccggcccc gggcgccgct
     3241 tcaccgcggg ctgcggcagc cgcggcgccc gcccaggcgg cgggaggcgc cgcgtgtggg
     3301 gcgcgagcgg tcaggagcac cgagggcaag agtcccccga tgtgcgcagg atgccagcgg
     3361 acctgctcga acccggcctg ggccagcagc cgcgcccacc cttccggccg caggccgaag
     3421 cggtggacca cggcggggtc cgggacgaac ggcagccagg ggcgcagcag cagggtcaag
     3481 cgtttgagcg gggcgtgcag ggtcgcgttc cggccgcgcg cgacgagcag gcctcccggc
     3541 ttcatccgcg ggcggagccg tgccagggcc tcggccggat cgtcgaggtg gtcgaggaca
     3601 tcccagagcg tcacgacgtc ccagccgtat ccgagcggga cggggctgag gatcgatccg
     3661 atggtcaccg agacgccgcg ggagcgcgcg gcgttcgccg cggccgggtc gatctcgatg
     3721 ccggcgacct ccgcgccatc accatgggcc gcttcgagga acgctccggc gccgcagccc
     3781 acatccagca cgcggtgcgg cgtgccgccg gcccaccgga ccgtgaagcg ccagcggcgc
     3841 gcggcgatcg tctggtcgat cccttcgagt gggagcccgg tgtcacggta acgggcctcc
     3901 tcctcggttc ggctcgacga ccggtcgttg aggacggtgc cgcagtcccc gcaattccgc
     3961 agcgccgtgt acgaccggcg cgccgaccag gccgttccgc ggcagacggg acaaggcggc
     4021 ggcccggagg agcgcgtgac cggcgcctcg gatgcgcgcg tggcaggctc gccgtgagtt
     4081 gccggctcgg cgtaagacgg gcggggcgtc cgcggatcgg cggggcgcgc ctctggcggc
     4141 tcgggtgagc gtggcgccgg cggctcgacg gggcaggggt cggccatcac ccccaacata
     4201 ggtcctcagg cggccgtgcg cacccggacc cgggtgaggt tgacgatggc gaccccgctc
     4261 agaatgacga gcgtgcctgc ccaaacgcgc catccgagcg gctcgtcgag gaggagccac
     4321 ccgagcacga gggcgatgac cgggttcacg taggcatagg tcgccacctt caccgcaggg
     4381 acgtggacga ggagccagtt gtacgccgaa taggcgacga gcgaccccgc gacgatgagc
     4441 caggcgaccg ccgccgccga cacggcgtcg aggcggaagc gcggggcctc ccccagcacc
     4501 agccccagca ggcacagggc ggcgccggcg atgagcattt gaagcgctgt cgccatgagc
     4561 ggcgacacat tccggagccg atgcttggag tagatcgacc cgaccgccca gcagaggttc
     4621 gccccgagaa gcgctgcctc cccccacatc gtcccgatcc cgagggaaag gctggcgacg
     4681 atcttcggcc aggtcaggag cagcatcccg gcgaagccgg cgacgaggcc cagccccccc
     4741 agaaaactca ggcgctcccc gtccttccgg aaatactcca ggatcgccat gacgaacggc
     4801 aggttggcga tgatgaccgc cgccatcccg gactccaccg tcatctccgc caccgtgacc
     4861 aggaggttcc cgccgaaaag cagcagcatt ccggtcagcg cgatcggtcc ccagaggtcg
     4921 cgcgacggca gcggaacacg gcggaacatc agccaggcga acaggatggc gccggcgatc
     4981 acgaagcgcg tcccggccat cagggcgggg ggcagggtgg ccacgccggc ccggatggcg
     5041 aggtaggtcg acccccacaa gatgtagacg gcggcgaagt tcagcgtgac gaggagtgcg
     5101 tggcggcggc gcgccgactc cgaaccctcc gggcccgggc cgccccccgc cggcgcctcg
     5161 gcggcgaggc tcacgatgcg gaccggcggc gcaagcgcat gatggcggcg gattctatcc
     5221 gacgcgggac cgcggcggag gccgcgcgag gcggtagaat gcggaagaaa tgagcgagcc
     5281 gtgcgcctac aaatggcccg cgctcctggt cgtcatgcga cacgcccaga gcgaggccaa
     5341 cttccaccgt gaatacctcg agcagcagga aagcctcgag acgcacgtca ccgtgcaggc
     5401 gcgggacgcc gacgtgcgtc tgacgagcga gggggagcgg caggcccgcg aggccgggcg
     5461 gtacctgcgc gaagagcacg gccgcttcga cgccgcctac atctcgccgt accgccgcac
     5521 ccgcgacacg gccgacttcg ccatcgcggc gctcgatccc gcgccggtca aggtgatcga
     5581 ggagcgcctg cgggagcggg acttcggcct catcgagagc ctgacgcggc acggcttcaa
     5641 gacgctccac cccggggagt gggagcgccg caagctcgtc gggaagtact actaccggcc
     5701 gccgggcggc gagtcgtacc cggacgtgaa tctgagagtc cactcgttcc tcgggacgct
     5761 ggtgcgcgag cacccggggc agcgggtcct cgtcgtcacc cacagcgtcg tcgtgctggc
     5821 gttccggcgg ctcatcgagc gcctcggcga ggaggaggtg ctggatctgg atcggagcaa
     5881 cgagccgaag aatgcgtcgc tgtcgatcta cgagtcgttt cccggcgaga cgatcggcac
     5941 cggcctcgcc ctgcagcagt ggaacttcgt ccccaaggag gcggcatgtc ggacctgaag
     6001 cttttcgtcg gcgagaagaa catctcctcc tggtcgatgc gagcctgggt ggcgatcacc
     6061 cacaaggggg tgccgttcga ggaggcgccc ctcccgctga agggggacaa ggaccgctcg
     6121 cgccgccgca ccgtctcccc caccggcaag gtgccggtgc tgcaccacaa gggagttgcg
     6181 atccccgatt cgctggcgat catcgagtac ctggaagaga cgttcccgcc gccgcagcat
     6241 ctggcgctct ggccgaggga tgccgcggag agggcgcacg cccgctggct ggccgccacg
     6301 atgcactccg ggttcatggg gatgcgcgag gggatgtcgt tcaacacctg cttcctgaag
     6361 acgcgcccgg cgccgaccgc cgcggccctc gccgacgccg cggagctgct ggggctcctg
     6421 gaggtgccgc tgtcgaaaag gcgagcgggc tccggaccgt acctgttcgg ggcgttcggc
     6481 gccgtggatg cgatgtacgc tccggccgcc gtccggctca tcgcgtacga agcgccggcc
     6541 ggcaagtcgc cgctggcaca cgcctacctg cgggacgtgc tcgccgcccc ggcggtcgag
     6601 ccgtggatgg acgcggcgcg cgcgctgccg ccggccgaga cctactgaat ggccgaaaag
     6661 gcggtgcgga tcgaggcggg cggggtgcgg ctctacgcca ttctgtccga caccgccacc
     6721 gcgagcgcct tctgggaggc gctgccgctc gaagggaagg tgcggctgta cggccaggcg
     6781 atcctcgtgg acgcgaagct cggagtcccc ctcgagccga tcgcgcgcgc ccaagtgaag
     6841 gccggcgacc tcgccttctg gccggacggc cccgccgtcg gcctgttctt cggccgcacc
     6901 ccggccagca ccggcgacga cccgatcgcc gcctcccccg tcaacgtctt cggccgcatc
     6961 accggcgact ccggccggct ggcgcgcgtc gtcgaggggg cgaaagtgaa gatcaccgct
     7021 ctggaaggct gaaccgctcc tcgccgcgcc gcgccggtgc cgctcagagc cggtcggtct
     7081 tgtactcgcg cacgtccgac gaggcgtcgg agatcaagtc gaagtggatg acgccgagat
     7141 cgcggccgtc gtcggtctcg acctcgacgc gccagcggcc cggctggtag ctcttcttgg
     7201 tcgcgatgac ccggaagccc ttgtcgcgcc cgccgttgat gtcgagccgg gcccgcccct
     7261 ggtcctgcca tccgccctgc cccttgacga gccagcgcac gtagatggcg tcgcggaagc
     7321 cggtcggcgc gaagacccgc gcgaagcagt agatgacgtc ccccgggcgc gccttgaagt
     7381 cctggtcgcc gtgctgccac ggcttccacc agggacgctg gtgccggagc tcgtaggtga
     7441 tcgcgccggc cggctgggcg gaggggggaa gcacctcgtg gtagatgccg atgtactgca
     7501 gcgacagcgg caccggcggg atcgccccga ggaaataggc ccccacgagg accgcctgga
     7561 tcccgaatcc cgccgaggcg acgcggctca gggaggacga gtcgctctcc attccctgac
     7621 cgtccgcggc ggcgctcttc cgtttcgaca accggcgcac cagccaggcg aacgcggcgc
     7681 acgacaggac gaccgccagc acgaagagcc acttgctgag gaagccgatc agcaccggca
     7741 gcagcagggc gagatacgac gtcaggcaga ggctcagaag cgcccagcgc acgaccggtc
     7801 cgcgctcgcg gaagcggggg agctcgttgg cgacgagcag ccccccgagg agcgacatga
     7861 acaggaacgc ggagaggccg ctgctgctct tgaggtacag cagcgtgaac gacgagagga
     7921 ggctcccgag gaggaaatgg atcgcgtcct cggagaagcg ccaggccttc gcgagaagac
     7981 gcgggggcgc ccagaccttg gcgcgctccc gctcctcgag gatcatcagc cagaccagga
     8041 gcgccaggta caccccctgc tggacgaggg tgaacgtgtc gtcgacgcgg ccgagggtga
     8101 cgatgtcgaa cagcatcccg gcggcgaaga aggtcgcgat ctccgccttg acgtgctgtc
     8161 cccggaatgc ctgcaggcgc tccagccgcc cggccggttt cgattccgtc acgtccatcg
     8221 gcgccccccc ggggacaacg taatctcgac ctgccgccgc gtcgagcggg ccggtggccg
     8281 ggacgccagg gacgcgattc gagccttcat gtgacgggga tcggcgtata ttcaggacag
     8341 gattgtcatg aaggtgctcg ccgtatcaaa cgcattcccg acccctgagc tcacggccca
//
LOCUS       DASRIQ010000461         6062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3857386, whole genome shotgun sequence.
ACCESSION   DASRIQ010000461 DASRIQ010000000
VERSION     DASRIQ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3857386"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..932
                     /locus_tag="VFD06_11260"
     CDS             276..932
                     /locus_tag="VFD06_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04149.1"
                     /translation="MASNRSPMNAKYGRFFVGALIWFGAVSWLAARGAFASGPGRPPA
                     AVAIAFATPILLFLLAMRVSPAWRARVVSIPPVFLIAVNGWRFVGLGFLMAWAEGLLP
                     GGFAWPAGLGDIAMAATAPFVAARVASDDRFRFGGVFLAWNLFGIADFLDAVFWGTLY
                     SWPGFTSPVTTALMQRHPFALIPCFFVPLVAMAHITLLEQRRAHSPGAESHRVPQPAL
                     "
     gene            1109..4294
                     /locus_tag="VFD06_11265"
     CDS             1109..4294
                     /locus_tag="VFD06_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04150.1"
                     /translation="MKLSKLIPAAGLVAAALAAGPAEAAIVNVTANITPLSPASLRNW
                     TADNEYVLGTVIYVEAGVTLTIEAGTVVRGLPDALTPGVNDPGTLVITRGAKIRAMGT
                     RLKPIIFTNQDDDNIGSNPGTAPYDNLADARDITGTWGGLVVLGRTYVANNTVAGPNA
                     AREIQIEGLTSPGGLGLYGNCAASSLFPSNCDDDDSGTIEYVSIRYGGFNVSPNNEIN
                     GLTLGAVGRETDIRFVEIFQNKDDAIEFFGGTVGVKNAVINGVGDDGLDWDEGFRGKV
                     QFVLQLQGTPGTDKSDKGGELDGGNGGDASQPRAIPTVYNTTFVGHGGQKDYTGHGEN
                     TVLHFRDNSGGRWYNSAFLDFGGAEALIEAGTVLGPSANTSRERSETAYATGGNCSVT
                     TSIACTTDAPCQPPATPGPEVCVQHYRGPASTFQLELEDNTFYCIRRQEDLAGVAPFP
                     PGGDRAPGVMVTFGICSGSGAQCLTAAGCPAGESCDDAPEDYGITLPGDANRFHHDNG
                     MLSNAALDNQYVACGSLPIRALVRDPDGDGIANPDPVISIDPRPSAGSPLLVTNRVTP
                     NDGFFQSAPYRGAFLDENWAWGWTNTYRLGYFPPKPQVLINANITPASPPSLMNWTPD
                     NEYVLGTVIYVESGVALDIRAGTIVRGLPDALTPGVNDPGTLVISRGAKLRAVGAPDK
                     PIIFTNRDDSNLDGHLGFSPYDNLADARDITGTWGGLVLLGRTYVANNTVAGPLAARE
                     VQIEGLTSPGGLGLYGNCAASALFPSNCDDDDSGTLDYVSIRYGGFNVSPNNEINGLT
                     LGGVGRETDISHVEVFQNKDDAIEFFGGTVGVKYAVINGVGDDGLDWDEGYRGKVQFV
                     LQMQGTPGTDKSDKGGELDGGNGGDASQPRAIPTVYNTTFVGHGGRKDYTGHGENTAL
                     HFRDNSGGRWYNSAFLDFGGAEALIEAGTVLGPSPNTSRERSETAYALGGNCSVTTSV
                     ACTTDAQCVPPATPGPEVCVQHYRGPGSPFQLELEDNTFYCIRRQEDLARATPFPPGG
                     DRAPGVMVTFGICSVAGTQYLTAAGCPAGQS"
     gene            4364..4990
                     /locus_tag="VFD06_11270"
     CDS             4364..4990
                     /locus_tag="VFD06_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04151.1"
                     /translation="MLSNALLDNRYQACDSLPIRTLVRDPDGDGAANPDPIIAIDPRP
                     AAGSSLLITNRMAPNDGFFTPVNYRGAFGSDNWAAAWTNTGTIGYAASCGPGLTVVPD
                     EVPNVQGVQPGRFTWDSPGLPGNMGILFYDVLRTSRSALATPISYAAATCVAADLFDA
                     MVNDGAVPAVGEAFYYEPRAENNCGDGEIGMTFPIIAPGSVLTGPSCD"
     gene            5119..>6062
                     /locus_tag="VFD06_11275"
     CDS             5119..>6062
                     /locus_tag="VFD06_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04152.1"
                     /translation="MEVDRTMQVHSPRPTGIRLLLLGALATIPVFGAGLAGTPGLAGL
                     DYAFRFASAIETDPKDRAKAQELALAEYMTAGAFEEASRRADKIEGWRRGVVYADLAA
                     SLARAGKRDEAMAFVNKAESIRGGVEGWQSPRISAHIAEALALLGDRERADTIVSGLA
                     KADPVQYTGRAAATESVGLAAGGDFDAAMKRLADLEGNDDFDTASWRTNGYLALARRD
                     DAPRKGRLEALDAALRSSATLPLPRQVEVALEASGISSGLDRRRDGRASVQSALRAVE
                     AAGVQTSEEIPYLIEIGRAWAALGEPEKAKASLFKAEGL"
BASE COUNT          967 a         2067 c         2099 g          929 t
ORIGIN      
        1 ctgcttcgcc tgggtcacca gatcggtcgc gtccgccagc cgctcctgca gcagccccgc
       61 cacctgaatc cgcaccttct cggacagatc gttcttcgtc ttgtacatgg ttcgcctccc
      121 gcttccgctg acgtcgtgtc agctctcgaa gaaagtgatg aggcccacat catggatctg
      181 aactgccggg aaagacaggc ggtgcgcgcc gccagttcgt tcgaggccgc tgcgccgcgc
      241 tgctatattc cgatcttcgc gcaaaggagg ccgccatggc gtcgaaccgc tccccgatga
      301 acgcaaagta tgggaggttt ttcgtcggtg cactgatctg gttcggcgcc gtctcctggc
      361 tggcggcgcg cggcgcgttc gccagcggac cgggccggcc gccggccgcc gtggcgatcg
      421 ccttcgcgac tccgatcctg ctgttcctcc tggcgatgcg cgtgtctccg gcctggcgcg
      481 cccgcgtcgt ctccattccg cccgtcttcc tcatcgccgt gaacggctgg cgattcgtcg
      541 gcctggggtt cctgatggcc tgggccgagg ggcttctgcc gggcggattc gcgtggccgg
      601 ccgggctcgg agacatcgcc atggccgcca ccgcgccttt cgttgcggcc cgggtggcct
      661 cggacgatcg cttccgcttc ggcggcgtgt ttctggcctg gaatctcttc ggcatcgcgg
      721 atttcctcga tgccgtcttc tggggaactc tctattcgtg gccgggcttc acctccccgg
      781 tgaccacggc gctcatgcag cgtcacccgt tcgcgctgat cccctgcttc ttcgtgccgt
      841 tggtcgcgat ggcgcacatc acgctcctgg agcaacggcg ggctcattcg ccgggtgccg
      901 aaagccaccg ggtcccgcag ccggccctct gaaaacgccg atcccgggcg aattcttcga
      961 gccagaggtc cggttcacgc agtcgtcaca gaagcgtcgg atcgccgtca ccccctcccg
     1021 gtaacttccg gtcggttttc gaagagcagc cacgctttgc ccgatccggg caggacttca
     1081 gtcttccgtt gtggggaggg actcgaacgt gaaactctcg aaactcatcc cggccgcagg
     1141 cctcgtcgcc gcggccctcg ccgccggccc ggccgaagcg gcgatcgtga acgtcacggc
     1201 gaacatcacg ccgctgagtc ccgccagtct gcggaactgg accgcggaca acgagtacgt
     1261 gcttggcacc gtcatctacg tcgaggcggg cgtcacgctg acgattgaag ccggaacggt
     1321 cgtgcgcggc ctgccggacg cactgactcc gggagtgaac gacccgggca cactggtcat
     1381 cacccgcggc gccaagatcc gcgccatggg gaccaggctg aagccgatca tcttcaccaa
     1441 ccaggacgac gacaacatcg gcagcaatcc cggcaccgct ccctacgaca acctggccga
     1501 cgcccgggac atcaccggga cgtggggcgg gctggtcgtg ctgggccgga cctatgtggc
     1561 aaacaacacg gtggcgggcc cgaacgcggc gcgcgagatc cagatcgagg ggttgacctc
     1621 ccccggcggc ctggggctgt acggcaactg cgctgcctcc tcgctgttcc cgtccaactg
     1681 tgatgacgac gacagcggca cgatcgaata cgtgtcgatc cgctacggcg ggttcaacgt
     1741 gtctccgaac aacgagatca acggcctgac gctgggtgcg gtgggccgcg agaccgacat
     1801 ccgcttcgtc gagatcttcc agaacaagga tgacgcgatc gagttcttcg gtggcacggt
     1861 cggcgtgaag aacgcggtga tcaacggcgt cggcgacgac ggtctcgact gggacgaggg
     1921 gttccgtggc aaagtccagt tcgtgctgca gctgcagggg acgccgggga ccgacaagtc
     1981 ggacaagggc ggtgagctgg acggcggcaa cggcggtgac gccagccagc cgcgggcgat
     2041 cccgaccgtg tacaacacca ccttcgtcgg gcatggtggg cagaaggact acaccgggca
     2101 tggcgagaac acggtgctgc acttccgcga caactcgggc gggcgctggt acaacagcgc
     2161 gttcctggac ttcggcggcg ccgaggcgct gatcgaggcc gggacggtgc tgggtccgtc
     2221 ggcgaacacg tcgcgggagc gctccgagac ggcgtacgcg acgggcggga actgcagcgt
     2281 gacgacgtcg atcgcctgca cgaccgacgc gccgtgccag ccgccggcga cgccggggcc
     2341 ggaagtgtgc gtgcagcact accgcggacc ggcatccacg ttccagctgg agctcgagga
     2401 caacaccttc tactgcatcc gccgccagga ggatctggct ggcgtggcgc cgttccctcc
     2461 gggcggcgac cgggctccgg gggtgatggt gaccttcggg atctgcagcg gctcgggggc
     2521 gcagtgtctg accgcggccg gttgtccggc gggcgagtcg tgcgacgatg cgccggagga
     2581 ctacggcatc acgctgccgg gcgacgccaa ccgcttccac cacgacaacg gcatgctgtc
     2641 gaacgcggcg ctcgacaacc agtacgtggc ttgcggctcg ctgccgatcc gggcgctggt
     2701 gcgcgacccg gacggtgacg gcatcgcgaa cccggacccg gtgatctcga tcgacccgcg
     2761 gccgtccgcc ggcagcccgc tcctggtcac caaccgggtc acgccgaacg acgggttctt
     2821 ccagtctgct ccctaccgcg gcgcgttcct ggacgagaac tgggcctggg gctggacgaa
     2881 cacctaccgt ctcggctact tcccgcccaa acctcaagta ttgatcaacg ccaacatcac
     2941 ccccgccagc ccgccgagcc tgatgaactg gactccggac aacgagtacg tcctggggac
     3001 cgtcatctac gtcgagtcgg gagtggcgct ggacatccgg gccgggacga ttgtgcgcgg
     3061 tctgcccgat gcgctgaccc cgggagtgaa cgacccgggc acgctggtca tcagccgcgg
     3121 cgccaagctg cgagccgtcg gggctcccga caagccgatc atcttcacga acagggacga
     3181 ctcgaacctc gacggccacc tcggattctc accctacgac aacctggccg acgcgcgtga
     3241 catcaccggg acgtggggcg gtctggtcct cctgggccgg acctacgtgg cgaacaacac
     3301 ggtggcggga ccactcgcgg cgcgcgaggt ccagatcgaa gggctgacct cgcccggcgg
     3361 cctgggactt tatggcaact gcgccgcctc ggcgctgttc ccgtccaatt gcgatgacga
     3421 cgacagcggc acgctggatt acgtctcgat ccgctacggc gggttcaacg tgtcgccgaa
     3481 caacgagatc aacggcctga cgctgggagg ggtgggacgc gagaccgaca tcagccacgt
     3541 cgaggtcttc cagaacaagg atgacgcgat cgaattcttc ggcggcacgg tgggcgtcaa
     3601 gtacgcggtc atcaacggtg tcggcgacga cggtctcgac tgggacgagg ggtaccgcgg
     3661 caaggtccag ttcgtgctgc agatgcaggg gacgccgggg accgacaagt cggacaaggg
     3721 cggcgagctg gacggcggca acggcggtga cgcgagccag ccgcgggcga tcccgaccgt
     3781 gtacaacacg accttcgtgg gccacggcgg gcggaaggat tacaccgggc acggcgaaaa
     3841 caccgctctg cacttccgcg acaactcggg cgggcgctgg tacaacagcg cgttcctgga
     3901 tttcggcggc gccgaggcgc tgatcgaggc ggggacggtg ctgggtccgt cgccgaacac
     3961 gtcgcgcgag cgctcggaga cggcgtacgc cctgggcggg aactgcagcg tgacgacgtc
     4021 ggtcgcttgc acgacggacg cgcagtgcgt gccgccggcg acgccggggc cggaagtgtg
     4081 cgtgcagcac taccgtggac cggggtcgcc gttccagctg gagctggagg acaacacctt
     4141 ctactgcatc cgtcgccagg aggatctggc gcgcgcgacg ccgttccctc cgggcggcga
     4201 ccgggctcct ggggtgatgg tgaccttcgg gatctgcagc gtggcgggga cgcagtacct
     4261 gacggcggcc ggatgtccgg cgggccagtc gtgagacgac gctccggagg actacggcat
     4321 cacgctgccg ggcgacgcca accggttcca ccacgacaac ggcatgctgt cgaacgcctt
     4381 gctcgacaac cggtaccagg cctgcgactc gctgccgatc cggacgctgg tgcgcgatcc
     4441 ggacggcgac ggcgcggcga acccggatcc gatcatcgcg atcgaccccc gccccgcggc
     4501 cggcagctcg ctgctcatca ccaaccggat ggcgccaaac gacgggttct tcaccccggt
     4561 caactaccgc ggcgcgttcg gatccgacaa ctgggccgca gcgtggacca acaccgggac
     4621 gatcggctac gcggcctcgt gtggccccgg cctgaccgtc gttcccgacg aggtcccgaa
     4681 cgtccagggg gtgcagcccg gcaggttcac ctgggacagc cccgggctgc cggggaacat
     4741 ggggatcctg ttctacgacg tcctccggac ctcgcgctcg gcgctggcga ctccgatcag
     4801 ctacgcggcc gcgacgtgcg tcgccgccga tctcttcgac gcgatggtca acgacggggc
     4861 ggttccggcg gtcggcgagg cgttctatta cgagccgcgg gcggagaaca actgcggcga
     4921 cggggagatc ggcatgacct tccccatcat cgcacccggg tcggtcctga ccgggccgtc
     4981 ctgcgactag tccgaggttg accggcccga cgctcgttcc gaggcgtggt cgggccgtga
     5041 cgtacgcggg ggcgggagcg ctgcaaggcg ctcccgcctt tccgaatgtt tgaataccgc
     5101 atcgagcgac cggccgccat ggaggtagat cgcaccatgc aggtccattc cccccgtccg
     5161 accggcatcc ggctcctgtt gctcggtgct ctggccacga tcccggtctt cggcgcgggc
     5221 ctcgccggga cccccgggct cgccgggctc gactacgcct tccgcttcgc gagcgccatc
     5281 gagaccgacc ccaaggaccg cgccaaggcg caggagctgg cgctcgcgga gtacatgacg
     5341 gcgggcgcgt tcgaagaagc gtcacgccgg gccgacaaga tcgagggctg gaggcgcggc
     5401 gtcgtctatg ccgacctggc ggcctccctc gcgcgagccg ggaagaggga cgaggccatg
     5461 gcgttcgtca acaaggcgga gtcgatccgc ggcggcgtcg aggggtggca gagcccgcgc
     5521 atctcggcgc acatcgcgga ggccctggct ctgctcggcg accgggaacg ggccgacacg
     5581 atcgtctccg gcctggcgaa agcggatccg gtgcagtaca ccggccgtgc cgcagcaacc
     5641 gaatcggtgg ggctcgccgc cggcggggac ttcgacgccg cgatgaagcg cctcgcggac
     5701 ctcgaaggga acgacgactt cgacaccgcc tcgtggagga ccaacggcta cctggcgctg
     5761 gcgcgacgcg atgacgcgcc ccgcaaggga cgtctggagg cgctcgacgc ggcgctgcga
     5821 tcctccgcca cgctgccgct gccgaggcag gtggaggtag cgctcgaggc ctccgggatc
     5881 tcttccgggc tcgatcgccg gcgggacgga cgtgcatcgg tccaatccgc cctccgcgcc
     5941 gtcgaggcgg cgggcgtgca gacctcggag gagatcccct acctcatcga gatcggcagg
     6001 gcctgggccg cgctcggaga gccggagaag gcgaaggcgt cgctcttcaa ggcggaaggg
     6061 ct
//
LOCUS       DASRIQ010000462        13640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3580083, whole genome shotgun sequence.
ACCESSION   DASRIQ010000462 DASRIQ010000000
VERSION     DASRIQ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3580083"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFD06_11280"
     CDS             <1..1152
                     /locus_tag="VFD06_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04153.1"
                     /translation="YYDRLFLSTLVGEQGPDTIARYYDTDPDGIDVTGFTPYKTTPNH
                     NLSRLFNSAPPSVRQIDRGLATPYSDEWSLGFEREVAPETAVAVRYIHRDYRDQLQDV
                     DINHEVVVDPVTGAPYDRVGVQFEIPGRPAFPGGPIVTPPIEIALPDGRPDLYIQNPF
                     FNQILRVENSNTATYSAVEVELRRRLARRWQMQGSYVYSRAQGNAEDFQSKVGNDPSV
                     TESEYGYLSFDQRHVVKVNAAVFLPADVQLGIATSWSSGLPYSVISRFFSSDNVGYTQ
                     FRTLFGTTTIAGNTRRFHPLPRNSERNDAVLDVNMSVRKSFVMGRTLGAVSLEVANLL
                     NTDDLHIVSYEPVTASSYDLAGGVPATPLQLDATRRFGRRFQIGFQFSF"
     gene            complement(1241..4954)
                     /locus_tag="VFD06_11285"
     CDS             complement(1241..4954)
                     /locus_tag="VFD06_11285"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN04154.1"
                     /translation="MATFAAAALCFSPRADAADRGALQGRVIDPAGNPLPGATVSLIP
                     PWLGTAESGAVTDARGEFRISGLPAGRGYQLRCAMPGFATTVLSDIDIPPGAETRLTV
                     ALAPADALVEEVRVRAAPPIVSLTETTTQSRFSSEFIDALPILGRNYQDVLTLAPGVS
                     DLDGDGNPNIHGARDTDVITLVDGVSTTDPFTGKVGAQLNIESIQEIEVKTSGATAEF
                     GRAQGGFANIITKSGGNQFEGSFKFFWRGSALDGDGAGIDDPSLHASVGEKGLRDLEF
                     NDFLPFLSLSGPIVRDRAWYFVTLEYIQREDPVNAVNTAFVTGLKEYRGFAKATWQVA
                     PATRLALSLNYDPQDYLNQGLNSLTREETGFTLGAGGTNISIRSVSTLSPSVVLEGTI
                     GFLDTRPSLTPNLGADTNGNGVMYVDRNGDGFTQASERDPGEDYDGDGAWDVWEDTYC
                     VDRTASLPCRNGILDVWNEPEGVFHTEDVDGDGRLTPYGTCEGTTREDIDCDGHRDDV
                     DEDVNGNGRLDGTEDLDGDRFLDRGGEDRNGNLVLDDTPFPTGDYPYGALRPQKQDRD
                     YFVNETTGITEGPYYETLEDTRRRATVRADLSVFVPDFRGSHDLRLGGIFERESFDRT
                     TGRHDIIGLQPPVAPLCDPEFGGCIGGRSASLAVVLPAERTVDGAADGSTGALYVHDS
                     WRPLPNLSLGLGLRYERETARAPGFTFFDPRQQRDTFDALVTLSGGELGRDDLRLGNR
                     DGLANQGILGDPMVYGGDGGLSGFGADLINALRLQAIRHLLRHRSNLEYSLGGLRGLY
                     SDLGTGDEIDPARLSALGVTTQQPEAFALTNDNLSPRLSVSWDPAANGLTKVFATWGR
                     YFDKLFLSTIVGEQGPDTVARYYEFDPDGVDVDLLVQQGFRATPDHHVGRIFGKSPPS
                     IRQVDRDLATPYSDEWTVGIEREVAPEVALALRFIRRDFRDQLQDTDINHEMPTDPVT
                     GGAYDLFGILFEIPIRPPGAPGRGSNVQLPDGRPDLFIQNPFFNQVLRVENANTARYS
                     AFEIELRRRMRRRWELQGSYVYSRAQGNAEDFQSKVGNDPSVTESEYGYLAYDQRHVV
                     KINAAVFLPKDVQLGIVTSWASGPPYSVIARFFAFDNTGYLQFRTLFGYTKVEDGAFR
                     FVPLPRNSERNDAVLNVNLALRKSFVIGRNLAGVSLEVDNLLNSDDLRVITYEPVNGS
                     GYDLGGLSPVTPLQLDATRRFGRRFQIGFQFAF"
     gene            5540..5746
                     /locus_tag="VFD06_11290"
                     /pseudo
     CDS             5540..5746
                     /locus_tag="VFD06_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
     gene            5743..6342
                     /locus_tag="VFD06_11295"
     CDS             5743..6342
                     /locus_tag="VFD06_11295"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HZN04155.1"
                     /translation="MTGRRGEAGLSLVEASLALALLALLVLASAPLCAATLSRARVGA
                     AAMEMAGTFARLRAQAIAEHRRVGLRFTTGGGPLSWAVYADGDGDGIRSDDIASGRDP
                     LLEPWRDLPSRYEGIDFGLLDAAVPEVPPQSGAIPPGADPIRFGSSGIVSFTPWGTAT
                     GGTVFVSDRRDTVCAVVLYGKTGRIRTFRLDRSQGRWID"
     gene            complement(6361..10104)
                     /locus_tag="VFD06_11300"
     CDS             complement(6361..10104)
                     /locus_tag="VFD06_11300"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN04156.1"
                     /translation="MTRRASGAHAIFAISLALLPLLTVSTPVAAADAGGVRGLVTDPS
                     GAPLAGVTVALSHPQRGTDQGGAVTDARGSFRIAGVPVGDGYSLTCSLSGFATVILSD
                     VQVSAGIVTGLTVTLSPERSLRETVKVRAAPPIVSLTETTTQSRFSSEFIDTLPILGR
                     NYQDILTLAPGVSDVDGDGNPNIHGARDTDVITLVDGVSTTDPYTGKVGAQLNIESIQ
                     EIEIKTSGATAEYGRAQGGFNNIITKSGGNQFEGSFKFYWRGSALDGDGAGIDDPSLH
                     GGIGESGLRDLTFNDYLPFVSLGGPIVRDRAWFFATLEYIQREDPVNAVNSAFVAGVK
                     EYRAFAKATLQATVNTRLALSLNYDPQEYLNQGLNSLTREETGFSLKAGGTNLTLRSV
                     SVLSPSVVLEGTLGFLDTHPALIPNLGYDTNANGLLFTDRNGDGFNQASERDAGQDFD
                     GDGAWDIWEDTHIRNGKIDLWEDTQPPGQDFGNTVIYTEDLDGDGRLTVRGGCEGATR
                     EDIDCDGRLDVKDEDINLNGRLDGSEDLDGDRHLDRGGEDRNGDERLNDAPFPGDLYP
                     YGGLRPEAPDRDYFIDETTGVTQGPYFEELEDTRQRSTVRADLSVFVPDFKGTHDFRF
                     GGLYERESFDRTTDRHDITALQPPVEPVCDEETGECGGGRPESLVIVLPTDRSVEGAA
                     SGTTGALYVQDTWRPLPNLSIGLGLRYEHETGRAPGFTAFDPREQRETFDTLVTLSGG
                     ELGRDDLRTGNRDGVANLGISGDPLLSGSEGGSSAFANDLINALRLQAIRHLTRHRSN
                     LEYSLDALAGLYGDLGSSGEIDPEQLSALGVTVQQPEAIGIVNDNLSPRLSVSWDPSG
                     DSRTKLFGTWGRYYDKLFLSTLVGEQGPDTVYRYYGYDSDGIDVFGGGSASLPTTVSN
                     HNVGRLFTKSPPSIRQVDRNLSTPYCDEWTLGIEREIAPEVALALRYIHRDFRDQLQD
                     TDINHEVLINPATGEPYDLFGIQYEVSGRFGPVRIQQPDGRPDLYIQNPFFNQVLRVE
                     NSNTARYSAVELELRRRMSRRWEMQGSYVYSRAQGNAEDFQSKVGNDPSVTESEYGYL
                     SFDQRHVIKINASIFLPRDFQLGLVTSWASGLPYSVISRFFASDNTGYAQFRTLFGFS
                     EVDPTSNTLEFRALPRNSERNDAVLDLNASVRKTFVLGRTTGAVSLEVANLLNSDDLR
                     VYTYEPVSGSGYDIGGLSAITPLQLDATRRFGRRFQIGFQFNF"
     gene            complement(10191..10832)
                     /locus_tag="VFD06_11305"
     CDS             complement(10191..10832)
                     /locus_tag="VFD06_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZN04157.1"
                     /translation="MIRGVIFDFDGTLIDSYEPIAESLNLVRAAFEKPPLPIDQVKDM
                     VGHGLEHLIEKSIGRDKVEEGVRVFREHYARICESRTKILPLVRQTLEELDRRGYVMG
                     IATNKPSYFARDILKALEIDHLFAEVLGPNDVARPKPDPEMLEIMMARLGLGTDEVVY
                     VGDMLLDIEVARKAGMAMYAVPSGSATRETLLTGRPDRLVSKFSDLLTYLPAL"
     gene            10947..11738
                     /gene="ccsA"
                     /locus_tag="VFD06_11310"
     CDS             10947..11738
                     /gene="ccsA"
                     /locus_tag="VFD06_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZN04158.1"
                     /translation="MDAAVWWLRGALVSYAAGSLLAFVPWRRGGAPPSRPIAPWCAGL
                     GALLHSVALLQTGWTLGRCPLGTLPEVLSVLAWAFTLAALWVWWRSRLDVVLAIEQPL
                     AFFVLLLSQVLPRESFLAGAAPSPAFVRFHLTVILFGVAALSITFAASILYLAVDRAL
                     KAKRPIAAFRRLPSLEACDLLAYRSLAVAFALLTLGILSGAVINDAMTGAPWTWRREE
                     TLAILAWILLGAIVLARLGWGWRGRRASILMVLGFGLILLRMLGF"
     gene            11741..13045
                     /gene="hemA"
                     /locus_tag="VFD06_11315"
     CDS             11741..13045
                     /gene="hemA"
                     /locus_tag="VFD06_11315"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZN04159.1"
                     /translation="MAILLYGLNHQSAPIALRERLVIADAELPATLLKLASAAGIEEA
                     FLLSTCNRTEILVSGEPGCASSLAPFLVQVGGGIGREELERHAYLHVDGAAVRHLFRV
                     TSSLDSMVVGEPQILGQVKTAYVLGRSTGTIRSVLEGLLQRAFAVAKQVRSSTGIGRA
                     PVSIAHAAATRAREIFGDLGGHAVVVVGAGKMARLAAQHVTAGGVGSLTVVNRSWQGA
                     DELARELHGTSVAWERLFDVLEGADVVIVSTAAPHHVIDLEEAQRLARARRGRPIFLL
                     DIAVPRNVDPRVNALENVYLYDLDDLRAVAEAGREERRREAEQAEAIVEQEAELYLDW
                     ARGLQASPTIVELRARLHDLGEAEMARFRGRLGPLSEEQSRVLEEFRIALLNKILHHP
                     TLALKRAGSRPEGGGAIALLREAFGLDAEEPGDEGKGSGRGA"
     gene            13038..>13640
                     /gene="hemC"
                     /locus_tag="VFD06_11320"
     CDS             13038..>13640
                     /gene="hemC"
                     /locus_tag="VFD06_11320"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZN04160.1"
                     /translation="MRDLVVGSRGSRLALAQAEWVVARLREQGLQARLEIVRTEGDRL
                     PTAPLHEIGGEGVFVKEIQTALLAGSIDLAVHSLKDLPTGGTEGLAVVAIPERADPRD
                     LLVAPGAPATLRALPTGARVGTGSPRRVAQARRIRPDLAFVPIRGNVDTRLDKRRRGE
                     CDALILAAAGIARLSASVEGAPLPPEEMLPAVGQGALAIEA"
BASE COUNT         1960 a         4648 c         4876 g         2156 t
ORIGIN      
        1 tactacgacc ggctgttcct cagcaccctg gtcggcgagc aggggcccga caccatcgcc
       61 cgttactacg acaccgatcc ggacgggatc gacgtgaccg ggttcacccc ctacaagacg
      121 acgccgaacc acaacctgtc acgcctgttc aacagcgccc cgccatcggt ccgccagatc
      181 gaccgcggcc tcgcgacccc ttacagcgac gagtggagtc tggggttcga gcgcgaggtc
      241 gccccggaga ccgcggtcgc ggtgcgctac atccaccgcg actaccgcga ccagctccag
      301 gatgtcgaca tcaaccacga ggtcgtcgtc gacccggtca cgggggcgcc gtacgaccgg
      361 gtcggtgtgc agttcgagat ccccggccgg cccgccttcc cgggagggcc gatcgtcacc
      421 ccgccgatcg agatcgcgct ccccgacggg cggcccgacc tctacatcca gaaccccttc
      481 ttcaaccaga tcctgcgggt ggagaactcc aacacggcga cctacagcgc cgtggaggtg
      541 gagctgcgcc ggcggctggc gcggcgctgg cagatgcagg gctcgtacgt ctactcgcgg
      601 gcgcagggga acgccgagga cttccagtcg aaggtgggaa acgacccgtc ggtgaccgag
      661 agcgagtacg gctacctctc cttcgaccag cggcacgtcg tgaaggtgaa cgccgccgtc
      721 ttcctgccgg ccgacgttca gctcgggatc gccacctcct ggtcctccgg gctgccgtac
      781 tcggtcattt cccgcttctt ctccagcgac aacgtcggct acacgcagtt ccggacgctg
      841 ttcggcacca cgacgatcgc ggggaacacg cgtcgcttcc acccgctgcc gcgcaacagc
      901 gagcggaacg acgcggtcct cgacgtcaac atgagcgtcc ggaagtcctt cgtcatggga
      961 cggacgctcg gcgcggtctc gctcgaggtg gcgaacctgc tcaacaccga cgacctgcac
     1021 atcgtgtcct acgagccggt caccgcgagc agctacgacc tggcgggggg cgttcccgcg
     1081 acgccgctgc agctcgacgc cacccgccgc ttcggccgcc gcttccagat cgggttccag
     1141 ttcagcttct gagccggcga ccgcgctctg ctccccgacg cgccgctgcg acgcatcgcc
     1201 gccctcgacg gcgtgctctg ggcggagcgg gtccagctcg ttagaacgcg aactggaatc
     1261 cgatctggaa ccgccggccg aagcgccggg tcgcgtcgag ctgcagcggc gtcaccgggc
     1321 tcaggccgcc gaggtcgtag ccgctcccgt tgaccggctc gtacgtgatg acgcgcaggt
     1381 cgtcgctgtt gaggaggttg tccacctcca gcgacacgcc ggcgaggttg cgaccgatca
     1441 cgaacgactt gcgcagggcc aggttgacgt tgagcaccgc atcgttccgc tcgctgttgc
     1501 gcggcagcgg gacgaagcgg aacgccccgt cctccacctt cgtgtacccg aacagcgtcc
     1561 ggaactggag atacccggtg ttgtcgaagg cgaagaagcg ggcgatgacc gagtagggag
     1621 gcccggacgc ccaggaggtc acgatcccca gctggacgtc cttcggcagg aagaccgccg
     1681 cgttgatctt caccacgtgc cgctggtcgt aggcgagata cccgtattcg ctctcggtga
     1741 cggaggggtc gttgccgacc ttcgactgga agtcctcggc gttcccctgg gcgcgggagt
     1801 agacgtagga gccctgcagc tcccagcggc gccgcatgcg gcggcgcagc tcgatctcga
     1861 aggcgctgta acgggcggtg ttggcgttct ccacacgcag cacctggttg aagaagggat
     1921 tctggatgaa cagatcggga cgtccatccg gcagctggac gttgctgccg cgccccggcg
     1981 cccccggggg gcggatcggg atctcgaaca ggatgccgaa cagatcgtac gctccgccgg
     2041 tgacgggatc ggtcggcatc tcgtggttga tgtcggtgtc ctggagctga tcgcggaaat
     2101 cgcgccggat gaagcgcagc gccagcgcca cctccggcgc cacctcccgc tcgatcccga
     2161 ccgtccactc gtcgctgtaa ggggtcgcga ggtcgcgatc gacctggcgg atcgaaggag
     2221 gtgacttccc gaagatccgg ccgacatggt ggtccggcgt ggcgcggaat ccctgctgca
     2281 cgagcaggtc cacgtccacc ccgtccggat cgaactcgta gtaacgcgcg accgtgtcgg
     2341 gcccctgctc gccgacgatc gtgctcagga acagcttgtc gaagtagcgt ccccaggtgg
     2401 cgaagacctt ggtgagcccg ttcgccgccg gatcccagga gaccgacagg cgcggcgaga
     2461 gattgtcgtt ggtcagggcg aacgcctccg gctgctgcgt cgtcaccccc agggccgaga
     2521 gccgcgccgg gtcgatctcg tcgccggttc cgaggtcgga gtacagcccg cgcagcccgc
     2581 ccagggagta ttcgaggttg ctccggtgcc ggagcagatg gcggatcgcc tgcaggcgca
     2641 gggcgttgat gaggtcggcc ccgaagccgg agagtccccc gtcgccgccg tacaccatcg
     2701 gatctcccag gatcccctgg ttggccaacc cgtcgcgatt gccgagccgg agatcgtccc
     2761 gtcccagctc gccgccgctc agggtcacga gggcgtcgaa ggtgtcgcgc tgctggcggg
     2821 gatcgaagaa ggtgaagccg ggggcgcgcg ccgtctcccg ctcgtaacgc aggccgagcc
     2881 ccaggctcag gttcggcaac ggtcgccagc tgtcgtgcac gtagagcgcc cccgtgctcc
     2941 cgtccgccgc gccatcgacg gtccgctcgg ccgggaggac gacggccagc gatgcggagc
     3001 gcccgccgat gcagccgccg aactccgggt cgcagagcgg cgcgaccggc ggctgaagac
     3061 cgatgatgtc gtggcgcccg gtcgtgcgat cgaacgactc gcgctcgaag atccccccga
     3121 ggcggaggtc gtggctgccc cggaagtcgg ggacgaagac cgagaggtcg gcccgcacgg
     3181 tggcccgccg gcgcgtgtcc tccagcgttt cgtagtacgg cccctcggtg atgccggtgg
     3241 tctcgttgac gaagtagtcc cggtcctgct tctgcggccg gagcgctccg tacgggtagt
     3301 cgccggtggg gaacggcgtg tcgtcgagga caaggttgcc gttgcgatcc tcgccgccgc
     3361 ggtcgaggaa ccggtcgccg tcgagatcct cggtcccgtc gagccggccg ttgccgttga
     3421 catcctcgtc cacgtcgtcc cggtggccgt cgcagtcgat gtcctcccgc gtcgtccctt
     3481 cgcaggtgcc gtagggcgtc aggcggccgt cgccgtcgac gtcctcggtg tggaagaccc
     3541 cttccggctc gttccagaca tcgaggatcc cgttgcggca cggcagcgag gcggtccggt
     3601 ccacgcagta cgtgtcctcc cagacgtccc atgccccgtc cccgtcgtag tcttcacccg
     3661 ggtcgcgctc gctcgcctgg gtgaagccgt cgccgttccg gtccacgtac atgacgccgt
     3721 tgccgttggt gtcggcgccg aggttcggcg tcagggacgg gcgcgtgtcg aggaacccga
     3781 tggtcccctc caggacgacc gacgggctga gggtggacac cgagcggatc gagatgttcg
     3841 tgccgccggc gcccagcgtg aacccggtct cttcgcgggt caggctgttg agcccctggt
     3901 tgaggtaatc ctggggatcg tagttgagcg acagggcgag gcgggtggcg ggggcgacct
     3961 gccaggtcgc cttggcgaag ccgcggtatt ccttcagccc ggtgacgaag gcggtgttca
     4021 ccgcgttcac cgggtcctcc cgctggatgt actccagcgt gacgaagtac caggcgcgat
     4081 ccctcacgat cgggccgctc agcgagagga aggggagaaa gtcgttgaac tccagatccc
     4141 ggaggccctt ctcgccgacg ctggcatgaa gcgaggggtc gtcgatgccg gcgccgtcgc
     4201 cgtccagcgc cgatccacgc cagaagaact tgaacgaccc ctcgaactgg ttgccgcccg
     4261 acttggtgat gatgttggcg aaccccccct gggcgcggcc gaactccgcc gtggcaccgg
     4321 aggtcttcac ctcgatctcc tgaatcgatt cgatgttgag ctgcgctcct accttgccgg
     4381 tgaacggatc ggtcgtcgag acgccgtcca cgagcgtgat cacgtcggtg tcgcgggcgc
     4441 cgtggatgtt gggattgccg tcaccgtcga ggtcgctgac gccgggggcg agggtgagca
     4501 cgtcctggta gttgcgcccg aggatcggca gtgcgtcgat gaactccgac gagaagcgcg
     4561 actgggtcgt cgtctccgtc agcgacacga tcgggggtgc cgcccgcacc cgcacctcct
     4621 ccacgagcgc gtcggccggc gcgagggcga ccgtcaggcg ggtctcggcc ccgggcggga
     4681 tgtcgatgtc ggagaggacc gtggtggcga agccgggcat cgcgcaccgc agctggtacc
     4741 cgcggccggc cggcaggccg gagatgcgga actccccccg tgcgtcggtc accgcgcccg
     4801 actccgcggt tccgagccac ggagggatca ggctcacggt cgcgccgggg agcggattcc
     4861 ccgcgggatc gatgacgcgt ccctggagcg ccccgcgatc ggcggcgtcg gcgcgcgggg
     4921 aaaaacagag cgcggcggcc gcgaacgtcg ccaccaggcc gacacgaaag gcatgaggcg
     4981 cgcgccctgg catggggacc accccgatgt ggggacattc tggtccgccg attcgggcgg
     5041 cgtcaatcgc gtccggcccg ggacgcgtcc ggcgccccgg cgtccgccgg cggccgcacg
     5101 agccggaccg gtggagccgc gccgcggccg gccgccacgc cccgcgtccg tgcgaacgcc
     5161 gcgcccggcg cctcctcccc gcgatccatc ggcgcgagtc ggcgccccgc gcccgcatgg
     5221 cacggccgct gcagcctccc gtcgcgccgc ggcacgagcg cggcgcaccg cagacttccg
     5281 agcttggggg agaacggaat gggtcgtcaa caaccgatcg gctctccaac cgaaagaccc
     5341 gttccttcct gtgacgcggg cgggggatcc gatgatcccc cctcccccaa gatttcctct
     5401 tccacttcaa cgcacggctt cacgacaagg agggacgatg ttccagacga tgcgaaggct
     5461 cgcagggatc ctcatggtgg cgcttctggt cgcggccgcg gtgccggcgc cggccgcggc
     5521 ggccgcggcc gccgctcccg cggcggagaa ggtcaacatc aacgcggcga cggccgacca
     5581 gttgacggcg ctcccgggga tcggtccgtc gtacgcgcag cggatcgtcg agtaccggga
     5641 gaagaacggg ccgttcaaga aggtcgagga tctgctcaac gtcaccggca tcggcgagaa
     5701 gacgttcgag aagatcaagg accggatcac cgtcggcggc aagtgacggg acgccggggc
     5761 gaggcgggcc tgtcgctggt cgaggcctcc ctcgccctgg cgctcctggc gctgctggtg
     5821 ctcgcgtcag cgccgctctg cgccgccacg ctgtcccggg cgcgggtcgg agcggcggcg
     5881 atggagatgg cgggcacctt cgcccgcctg cgggcgcagg cgatcgccga gcaccgccgg
     5941 gtcggactcc ggttcacgac cggtggcggg ccactctcct gggcggtcta cgccgacggg
     6001 gacggcgacg ggatccgatc cgacgacatc gcctcgggac gcgacccgct cctggagccc
     6061 tggcgcgatc tgccgtcgcg ctacgagggg atcgacttcg gcctgctcga cgccgcggtg
     6121 ccggaggtgc cgccgcagag cggggcgatc cctccgggcg ccgatccgat ccggttcgga
     6181 tcctccggca tcgtctcgtt cacgccgtgg ggaaccgcga ccggtgggac cgtcttcgtc
     6241 agcgaccggc gcgacacggt ctgcgccgtc gtcctgtacg ggaagaccgg gcggatccgg
     6301 accttccgcc tcgaccgatc ccaggggcgc tggatcgact gaccggcgcc gcccggcgga
     6361 tcagaagttg aactggaacc cgatctggaa gcgccggccg aagcggcggg tggcgtcgag
     6421 ctgcagaggg gtgatcgcgc tcagtccgcc gatgtcgtag ccgctgccgg agaccggctc
     6481 gtaggtgtag acgcgcaggt cgtcgctgtt gagcaggttc gcgacctcga gcgacacggc
     6541 gccggtggtg cgcccgagga cgaacgtttt ccgcacgctg gcattgagat cgagcaccgc
     6601 gtcgttgcgc tcgctgttgc gcggcagcgc gcggaattcg agggtattcg acgtgggatc
     6661 gacctcgctg aagccgaaga gcgtccggaa ctgcgcgtac ccggtgttgt ccgaggcgaa
     6721 gaagcgggag atgaccgagt aggggagacc ggacgcccac gaggtgacga gcccgagctg
     6781 gaagtcgcgc ggcaggaaga tcgacgcgtt gatcttgatg acgtgccgct gatcgaagga
     6841 gaggtagccg tactcgctct ccgtcaccga cgggtcgttt ccgaccttcg actggaagtc
     6901 ctcggcgttc ccctgggcgc gcgagtagac gtacgatccc tgcatctccc agcggcgcga
     6961 catcctccgc cgcagctcca gctcgacggc gctgtagcgg gcggtgttgg agttctccac
     7021 gcgcagcacc tggttgaaga acgggttctg gatgtagagg tcgggtcgac cgtccggctg
     7081 ctggatccgg accggtccga agcgtcccga cacctcgtac tggattccga acaggtcgta
     7141 cggctcgccg gtggccgggt tgatcagcac ctcgtggttg atgtcggtgt cctggagctg
     7201 gtcgcggaaa tcgcggtgga tgtagcgcag cgccagcgcc acctcggggg cgatctcccg
     7261 ctcgattccg agcgtccact cgtcgcagta gggggtcgag aggttgcggt ccacctggcg
     7321 gatcgacggc ggggacttgg tgaacagccg gccgacgttg tggttcgaca ccgttgtggg
     7381 cagcgacgcg ctgccgcctc cgaagacgtc gatgccgtcg gagtcgtagc cgtaatagcg
     7441 gtacaccgtg tccggcccct gctcgccgac cagggtgctg aggaacagct tgtcgtagta
     7501 acgcccccag gtgccgaaca gcttggtccg cgagtcgccg gacggatccc aggagaccga
     7561 caggcggggt gagaggttgt cgttgacgat gccgatcgcc tcgggctgct gcacggtgac
     7621 gccaagcgcc gagagctgct ccggatcgat ttcgccgctg ctcccgaggt cgccgtacag
     7681 gccggcgagc gcgtcgaggg agtactcgag gttgctgcgg tggcgcgtca ggtgccggat
     7741 cgcctgcagc cgcagggcgt tgatcaggtc gttggcgaac gcggagctgc ccccctccga
     7801 gccggacagg agcggatcgc cggagatccc gaggttcgcg acaccgtccc gattgccggt
     7861 gcgcagatcg tcgcggccca gctcgcctcc gctcagcgtg acgagggtgt cgaaggtctc
     7921 tcgctgctca cgcgggtcga aggccgtgaa gccgggcgcc cgccccgtct cgtgctcgta
     7981 acgcaacccc aggccgatgc tcaggttcgg cagcggccgc caggtgtcct ggacgtacag
     8041 cgccccggtg gtcccggacg ccgccccctc gaccgatcga tcggtcggca ggacgatgac
     8101 cagcgattcg gggcggccgc cgccgcactc cccggtctct tcgtcgcaga ccggctccac
     8161 tgggggctgc agcgccgtga tgtcgtgccg atcggtcgtg cggtcgaagg actcccgctc
     8221 gtacagccct ccgaagcgga agtcgtgggt ccccttgaaa tcggggacga agaccgacag
     8281 gtcggcgcgc accgtcgatc gctgccgggt gtcctcgagc tcttcgaagt agggtccctg
     8341 ggtgacgccg gtcgtctcgt cgatgaagta gtcgcgatcg ggggcctccg gacgcagccc
     8401 gccgtacgga tagagatcgc cggggaacgg cgcgtcgttg agcctctcgt cgccgttgcg
     8461 gtcctctccg ccgcgatcga ggtgccggtc gccgtcgagg tcctcgctcc cgtccagccg
     8521 gccgttgagg ttgatgtcct cgtccttcac atcgagccgg ccgtcgcagt cgatgtcctc
     8581 gcgggtcgcc ccctcgcagc cgccgcgcac cgtcaggcgt ccgtcgccgt ccaggtcctc
     8641 ggtgtagatg accgtgttgc cgaagtcctg gcccggcggc tgggtgtcct cccagaggtc
     8701 gattttcccg ttgcggatgt gcgtatcctc ccagatgtcc caggcgccgt cgccgtcgaa
     8761 gtcctggccc gcgtcgcgct cgctcgcctg gttgaacccg tctccgttcc gatcggtgaa
     8821 gagcaagccg ttggcattcg tgtcgtagcc caggttcggg atcagggccg gatgggtgtc
     8881 gagaaaaccc agcgtccctt cgaggacgac cgacgggctg aggaccgaca ccgagcgcag
     8941 ggtcaggttc gtcccgcccg ccttcaggct gaatccggtc tcctcgcggg tcaggctgtt
     9001 gagcccctgg ttgaggtact cctgggggtc gtagttgagc gacagtgcca ggcgcgtatt
     9061 gacggtggcc tgcagggtcg ccttggcgaa ggcgcggtat tccttcaccc cggcgacgaa
     9121 ggcggagttc accgcgttca ccgggtcctc cctctggatg tattcgaggg tggcgaagaa
     9181 ccaggcgcgg tccctgacga tgggcccgcc gagcgacacg aagggaaggt agtcgttgaa
     9241 ggtcagatcg cgcagccccg actcgccgat gccgccgtgg agcgacgggt cgtcgatgcc
     9301 ggcgccgtcc ccgtccaggg ccgatccgcg ccagtagaac ttgaacgacc cctcgaactg
     9361 gttcccgccg gacttggtga tgatgttgtt gaagcccccc tgggcgcggc cgtactccgc
     9421 ggtggcgccc gaggtcttga tctcgatctc ctggatcgac tcgatgttga gctgggcgcc
     9481 gaccttgccg gtgtacggat cggtcgtgga gaccccgtcc acgagggtga tgacgtcggt
     9541 gtcgcgggcg ccgtggatgt tggggttgcc gtcgccgtcc acgtcgctga cgccgggagc
     9601 gagggtgagg atgtcctgat agttccgccc gaggatcggc agcgtgtcga tgaactccga
     9661 cgagaagcgc gattgcgtcg tggtctcggt cagcgacacg atcggcgggg ccgcccggac
     9721 cttgaccgtc tcccgcagcg accgttccgg gctcaaggtg acggtgagcc cggtgacgat
     9781 cccggcggac acctgtacgt cggacaggat gacggtggcg aacccggaca gggagcacgt
     9841 caggctgtat ccgtcgccga ccgggacgcc ggcgatccgg aacgacccgc gggcgtcggt
     9901 gaccgcgccc ccctgatcgg tgccgcgctg cgggtggctc agggcgacgg tcacgccggc
     9961 gagcggcgcg ccggacggat cggtgacgag gccgcgcacc ccccccgcat cggccgcggc
    10021 gaccggcgtg gaaaccgtga ggagggggag aagcgcgaga ctgatggcga agatggcgtg
    10081 ggcgccggac gcgcgcctcg tcatcggaac gacccccctg gaaacgcgta ttgtcgcccg
    10141 gacgccgcgc ggcggtcaat cacgacggcc gagggcctgg acccaggatc ctagagggcc
    10201 ggcaggtagg tcaggagatc ggagaacttc gacaccagcc ggtcggggcg gccggtcagg
    10261 agcgtctccc gcgtcgcgga gccgctcggc acggcgtaca tcgccattcc ggccttgcgg
    10321 gccacctcga tgtccaggag catgtcgccg acgtagacca cctcgtcggt gccgaggccc
    10381 agccgcgcca tcatgatctc cagcatctcg gggtccggtt tgggccgcgc cacatcgttc
    10441 gggcccagca cctcggcgaa gaggtggtcg atctccagcg ccttgaggat gtcgcgcgcg
    10501 aagtacgacg gcttgttggt ggcgatcccc atgacgtagc cgcgccggtc caactcctcg
    10561 agcgtctggc gcaccagcgg caggatcttc gtgcggctct cgcagatgcg ggcatagtgc
    10621 tcgcggaaca cccgcacccc ctcctcgacc ttgtcgcgcc cgatcgattt ctcgatcagg
    10681 tgctcgaggc cgtgcccgac catgtccttc acctgatcga tcgggagcgg gggcttctcg
    10741 aacgccgccc gcaccaggtt caggctctcg gcgatgggct cgtaggaatc aatcagggtg
    10801 ccgtcgaagt cgaagatgac gccgcggatc aagcagttcc ctcagaaacg gcgggaatcg
    10861 tagcaccccg ccgctcggcc gatcaagacg agtgatacac tccggcccca tccgcgggcg
    10921 tccaggcgaa cgcccccgga gaaccgatgg acgcggcagt gtggtggctg agaggggccc
    10981 tggtctcgta cgcggccggg tcccttctgg cgttcgtccc ctggcgccgg ggaggggccc
    11041 ccccgtcccg gccgatcgct ccctggtgcg ccggcctcgg cgccctgctg cattcggtcg
    11101 ccctcctcca gaccggctgg acgctcgggc gctgcccgct cgggaccctg ccggaggtcc
    11161 tgtcggtgct cgcctgggcc ttcaccctcg cggccctgtg ggtctggtgg cgttcccggc
    11221 tcgacgtcgt cctggcgatc gagcagccgt tggccttttt cgtgctcctg ctgtcccagg
    11281 tgctgccgcg cgaatccttc ctggccggcg ccgcgccgtc tccggcgttc gtccggttcc
    11341 atctcaccgt gatcctgttc ggcgtcgccg ccctgtcgat caccttcgcc gccagcatcc
    11401 tgtacctcgc ggtcgatcgg gccctcaagg cgaagcggcc gatcgccgcg ttccggcggc
    11461 tcccgtcgct cgaggcgtgc gacctcctgg cgtaccgctc cctcgccgtc gccttcgcgc
    11521 tcctgacgct ggggatcctg agcggcgccg tgatcaacga cgccatgacc ggcgccccat
    11581 ggacctggcg gagggaagag accctcgcga tcctcgcctg gatcctgctc ggggcgatcg
    11641 tcctggcgcg gctcggatgg ggctggcgcg gccggcgtgc ctccatcctc atggtgctcg
    11701 ggttcgggct cattctcctg agaatgctgg ggttctaggg atggcgatcc tcctgtacgg
    11761 cctgaaccac cagagcgcgc cgatcgcgct gcgcgaacgt ctggtgatcg ccgacgcaga
    11821 gctccccgcc acgctcctga agctcgcgtc cgccgccgga atcgaggagg ccttcctgct
    11881 gtcgacctgc aaccgcacgg agatcctggt gagtggcgag cccgggtgcg cgtcgtcgct
    11941 ggctcccttc ctggtgcagg tcgggggcgg gatcggccgc gaggagctgg agcggcacgc
    12001 ctacttgcac gtggacggag ccgccgtgcg acacctgttc cgggtgacgt cgtcgctcga
    12061 ttccatggtg gtgggggagc cgcagatcct cggccaggtg aagacggcgt acgtgctggg
    12121 gcgatcgact ggcaccatcc ggagcgtcct cgaagggctc ctgcagcgcg ccttcgcggt
    12181 cgcgaagcag gtgcgcagct cgaccgggat cgggcgcgcg ccggtgtcga tcgcccacgc
    12241 cgccgcgacc cgtgcgcggg agatcttcgg cgacctcggc ggccacgcgg tcgtggtcgt
    12301 gggggccggc aagatggcgc gtctcgcggc gcagcacgtg acggcgggag gggtcggctc
    12361 cctcacggtg gtcaaccgct cctggcaggg ggcggacgag ctggcgcgcg agctccacgg
    12421 gacgtcggtc gcgtgggagc ggctgttcga cgtcctggag ggggcggacg tcgtgatcgt
    12481 gtccaccgcc gcgccgcacc acgtcatcga cctcgaggag gcgcagcgcc tcgcccgggc
    12541 acggcgcggt cggccgatct tcctgctcga catcgcggtg ccgcgcaacg tcgacccgcg
    12601 ggtgaacgcg ctcgagaacg tctatctcta cgatctcgac gatctgcgcg cggtggcgga
    12661 ggcggggagg gaggagcggc ggcgcgaggc cgagcaggcg gaggcgatcg tcgagcagga
    12721 ggcggagttg tacctcgact gggcgcgagg tctccaggcg tccccgacca tcgtcgaact
    12781 gagagcccgc ctgcacgatc tgggagaggc ggagatggcc cgcttccgcg ggcggctcgg
    12841 tccgttgtcg gaggagcagt cgcgggtcct cgaggagttc cgcatcgccc tcctgaacaa
    12901 gatcctccac catcccaccc tggcgctgaa gcgggccgga tcgcggccgg aagggggagg
    12961 ggcgatcgcg ctgctgcggg aggcgttcgg cctcgacgcc gaggagccgg gggacgaagg
    13021 gaaggggagc ggacggggtg cgtgatctcg tcgtcggcag ccgcggcagc cgcctcgccc
    13081 tggcgcaggc ggagtgggtc gtcgcgcgcc tgcgggagca gggtcttcag gcgcgcctcg
    13141 agatcgtccg caccgagggg gaccggctgc cgacggcgcc cctccatgag atcggcggcg
    13201 agggggtgtt cgtcaaggag atccagaccg cgcttctggc ggggagcatc gacctcgcgg
    13261 tgcacagcct caaggacctg ccgaccggcg ggacggaggg gctcgcggtc gtcgctatcc
    13321 cggagcgggc cgacccgcgc gatctgctcg tggccccggg cgctccggcg acgctccggg
    13381 cgcttccgac cggcgcccgc gtcgggaccg ggtcgccgcg ccgggtggcg caggcgcggc
    13441 gcatccgccc cgacctcgcc ttcgtgccga tacgcgggaa cgtcgacacc cggctcgaca
    13501 aacgccgccg cggggagtgc gacgccctga tcctggccgc ggccgggatc gcgcggctgt
    13561 cggcatcggt ggagggggcg ccgctgccgc cggaggagat gctccccgcg gtcggtcagg
    13621 gcgcgctggc gatcgaggcg
//
LOCUS       DASRIQ010000463         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3857618, whole genome shotgun sequence.
ACCESSION   DASRIQ010000463 DASRIQ010000000
VERSION     DASRIQ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3857618"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFD06_11325"
     CDS             <1..900
                     /locus_tag="VFD06_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04161.1"
                     /translation="AAVLTLGLGIGSTTIIFSILEAVLLRPWPYPGVERLVVPASVNL
                     ATWDRWSVTYADFQDWQEEGIFDGVAVYQTSGLDLNPGAEPVRITTAWVSPDFFRVLG
                     VPLALGRDFRPEEFVPDTERHIILSDPLWRSAFGGDAGVLGRTVHLSGVPFTVAGVMP
                     PGFAWPREATAWLPLRQKLPDPDLTRRDNFIFSGIARLRQDQPLEPTRARLAALAKRI
                     EQDDPMARRGISLTAVPLGEWIVGPDLSRALWLLWRRSGPCCSSAASTSPTCCWPGAR
                     PGGTSWRCSRRSAPGGRGSSGSS"
     gene            783..2363
                     /locus_tag="VFD06_11330"
     CDS             783..2363
                     /locus_tag="VFD06_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN04162.1"
                     /translation="MGCVNVANLLLARGSARRHELAVLAALGARRARIVRQLMIESLL
                     LAVAGGAAGIVLAVWGLGSVAALGPADVPRLAEAHVNTPVALFACLVSLLTALLTGIL
                     PALQISSLQPGQAIDTGGRGGTGSRRGTRLRDVLVAAELALSLVLLTGASLMARSLSH
                     LQRVDAGVEVENLETATVTLPGARYKTDEARWSFYESLIARLEATPGVRAAAAGSASP
                     LGGGGFYLGRTFLAEGRPAPPAGPELNAMWNVVTPGYFETTGTVLKKGRDFTVRDEAG
                     STPVAIVNEAFARAMFPGEEALGRRAKSWRDENVLREIVGVVQDVRYFGAGDEIRPLF
                     YVPHRQNTWGAMVVTVRAPGAGPTGTPSPAASLRAALATLDKDLAIGDVRTMSQSMTA
                     SMARPRFNTLLWSTFAALALLLAAVGLYGIMSYAVVQRTREIGVRIALGARAGDVHRL
                     MLRRTLRLLAFGLLAGTAGALAVTRLMAGLLFEVAPWDQAAFAAGSLILASVAIIAAY
                     LPARRAARVDPMTALRHE"
BASE COUNT          323 a          965 c          907 g          424 t
ORIGIN      
        1 gcggccgtgc tgaccctcgg cctcggcatc ggctcgacga cgatcatctt cagcatcctg
       61 gaggcggtcc tgctgcgccc ctggccgtat cccggggtgg agcgcctcgt cgtgcccgcc
      121 tcggtcaacc tggccacgtg ggaccgctgg tcggtcacct acgccgattt ccaggactgg
      181 caggaggaag ggatcttcga cggcgtcgcg gtctaccaga cctccggcct cgacctcaac
      241 ccgggggccg agccggtgcg catcacgacc gcctgggtga gccctgattt cttccgcgtt
      301 ctcggcgttc ccctggccct cggccgtgac ttccgacccg aggagttcgt gcccgacacc
      361 gagcgccaca tcatcctgag cgatcccttg tggcggtcgg ccttcggggg cgacgccggg
      421 gtcctcggac gcaccgtcca cctttccggc gtgccgttca cggtggcggg cgtcatgccg
      481 cccggcttcg cctggccgcg cgaggcgacg gcctggctgc cgctgcggca gaagctcccc
      541 gatcccgatc tcacgcgccg ggacaacttc atcttctccg gcatcgcgcg cctgcggcag
      601 gaccagccgc tcgagccgac ccgcgcgcgc ctggcggcgc tcgcgaagcg gatcgagcag
      661 gacgacccga tggcccgcag gggaatctcc ctgacggccg tacctctcgg agagtggatc
      721 gtcggtccgg acctgtcacg cgccctttgg ctcctttggc ggcggtcggg accgtgctgc
      781 tcatcggctg cgtcaacgtc gccaacctgc tgctggcccg gggctcggcc cggcggcacg
      841 agctggcggt gctcgcggcg ctcggcgccc ggagggcgcg gatcgtccgg cagctcatga
      901 tcgagagcct cctcctcgcc gtcgcgggag gggcggccgg gatcgtgctc gccgtctggg
      961 gtctcggaag cgtcgccgcc ctggggccgg cggacgtgcc gcggctggcg gaggcgcacg
     1021 tcaacacgcc ggtcgctctc ttcgcctgcc tggtgtcgct cctgaccgca ctcctgaccg
     1081 ggatcttgcc ggccctgcag atctcctccc tgcagccggg gcaggcgatc gacacgggcg
     1141 gccgcggcgg caccggctcg cgccgcggca cgcggctgcg cgacgtcctg gtcgcggccg
     1201 agctggccct gtcgctcgtc ctcctgacgg gtgccagcct catggcacgg agcctctcac
     1261 acctgcagcg cgtcgacgcc ggggtcgagg tcgaaaatct ggagacagcc accgtgacgc
     1321 tgccgggcgc gcgctacaag accgacgagg cgagatggtc cttctacgag tcgctcatcg
     1381 cgcggctcga ggcgaccccg ggcgtccggg cggcggccgc cggctccgcg agcccgctgg
     1441 gcggcggcgg cttctacctc ggccgcacct tcctggcgga ggggcgtccc gccccgcccg
     1501 caggccccga actcaatgcg atgtggaacg tcgtcacgcc cggatacttc gagacgacgg
     1561 gaacggtgct caagaaggga cgcgacttca cggtgcgcga cgaggccggc tcgacgccgg
     1621 tcgcgatcgt caacgaggcg ttcgcccggg cgatgttccc gggcgaggaa gcgctcggcc
     1681 ggcgggcgaa gtcctggcgg gacgaaaacg tcctgcggga gatcgtcggc gtggtccagg
     1741 atgtgcgcta cttcggcgcc ggggacgaga tccggcctct cttctacgtg ccgcaccgcc
     1801 agaacacctg gggcgcgatg gtcgtgaccg tgcgcgcccc cggtgccggg cccaccggaa
     1861 cgccgagccc cgcggcgtcg ctgcgcgcgg cgcttgccac cctcgacaag gacctggcga
     1921 tcggcgatgt ccgcaccatg agccagtcga tgacggcctc gatggcccgg ccgcgcttca
     1981 acactctgct gtggtcgacg ttcgcggcgc tggcgctcct cctggcggcg gtcgggcttt
     2041 acggcatcat gtcgtacgcg gtcgtgcagc gcacgcggga gatcggggtg cgcatcgccc
     2101 tcggagcgcg cgccggggac gtccaccggc tgatgctgcg ccgcacgttg cggctgctgg
     2161 cctttggcct tctggccggg accgcgggcg cgctcgccgt cactcgcctc atggccgggc
     2221 tgctcttcga ggtcgccccc tgggaccagg cggcgttcgc cgcgggctcc ctcatcctcg
     2281 cgtccgtcgc gatcatcgcg gcctacctac cggcccggcg cgccgcgcgg gtcgatccga
     2341 tgacggcgct gagacacgag tagagatcgt acccgcacga ttcgtcgcgg cgtggaggat
     2401 ctgcttgtcc tccccttgcg cgccttcgcc ctccccaccc actccgccag ggatttcggg
     2461 cctgattggt tctcttggaa tactcatcac agcttcgaac ggcgtaattg tgcccatcct
     2521 ctcagcgaac gcagctgacg ttagtagacc gagcgcggtt gtgcgcctca cgaacatcct
     2581 cgcctccact cgcgaccctc aggctgcgtc aggaagcag
//
LOCUS       DASRIQ010000464         9415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3857850, whole genome shotgun sequence.
ACCESSION   DASRIQ010000464 DASRIQ010000000
VERSION     DASRIQ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3857850"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /gene="wecB"
                     /locus_tag="VFD06_11335"
     CDS             <1..1012
                     /gene="wecB"
                     /locus_tag="VFD06_11335"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZN04163.1"
                     /translation="ETLYDEERPALVLVQGDTTSALCAGLAAFHRRIAVGHVEAGLRS
                     FNRFHPYPEEANRRMLSSVTDLHLAATPWSAANLEREGVRREEIVVTGNTVIDALLES
                     LDRPDRLGRQTAFPRQAGERLVLITLHRRENWMHGAQGAGAPGRAVQESPLEETLAAI
                     GDAATRFPAARFVYPVHRNPRVQEPARRILGGRPNVHLLDPLPYLPFVDLLGRASLIL
                     TDSGGIQEEAPSLGVPVLVARETTERPEGVEAGTNVLVGTRRDAVLAAMTKHLERPFE
                     SPGQRPRPNPFGDGQASERIRQAVLHHLGLGEQATEFVPPMASSSLSSAPRLEGAGAR
                     RP"
     gene            1013..2245
                     /locus_tag="VFD06_11340"
     CDS             1013..2245
                     /locus_tag="VFD06_11340"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZN04164.1"
                     /translation="MSETKTKTKITLPSDADCSGRTFGAEELAILQRVIASGTLNCTK
                     GTVVREFEKRFGESLGVPFCRTTTSGTAALHAAVAAIDPEPGDEIITTPITDMGAITP
                     ILYQTAIPVFADVDPLTYNLTAETIAKKITRRTKAVMVTHLFGNACDMDPIMALARER
                     NIKVIEDCAQAFHCTYKGKPVGTIGDLGCFSLQQTKHMTAGEGGMVVARDEALSRRAR
                     LFIDKAWGYGDPKPDHYFLALNYRMTEVVGAVALAQFDKLEAMVKHRVAMADALSKKI
                     EGVKGIAAPKITPGCKPVYWKYPLRIDTNLVRGGADAFGARLKEDGVFTAPRYIQKLA
                     FECEVLRDQRTFGNSRFPFVGEHRNGDAPVVYDKTDTPGAVEALAGVVVLPWNEKYTP
                     EHVEFIAASIRQASKELT"
     gene            2280..3287
                     /locus_tag="VFD06_11345"
     CDS             2280..3287
                     /locus_tag="VFD06_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN04165.1"
                     /translation="MGKVGKGDAVRFGVVGCGRIAQSHLQALGTLSDARLTAAVESRK
                     PAGEAVAQEYKCALYSDYREPALLEQIDAAIICTPPSLHHDIARHFLERGVHVLCEKP
                     LTIESAEARDLVALSDEKDLVLMMASKFRYVDDIIKAKAIIESGILGRIILYENTFCS
                     KVAMSDRWNANKEVSGGGVLIDNGSHSADIARYLLGPIADVQAQNGLPTQGLDVEDTV
                     RVQFVTQGGVIGTFDLSWSITKETPHYVQVFGSEGTLQVGWSGSKYRQDGSSRWVPFG
                     TGYDKLKAFSRQLQNFIGCIKGREAPVISAGDSLASVQVIETAYRSAGRNNWLRVEPA
                     A"
     gene            3287..3937
                     /locus_tag="VFD06_11350"
     CDS             3287..3937
                     /locus_tag="VFD06_11350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN04166.1"
                     /translation="MKPARVHPTALVEEGVVLGHDTSVWDGVHIRHGATIGHHCIVGE
                     KTYIAYDVSIGNYVKINAVVYICANVSIGDFCMIAAHTVFANDRFPRSGNRELTGLET
                     SDVTEETLTTRLERGVTIGANATIGPGITLGEFSMVGMGSVVTRDVPAFALVHGSPAK
                     VAGWLSACGHPLLRGRDLPAPDDAAASRDLPCERCGRDYRFDGRALSPRVPIVSAS"
     gene            3934..5349
                     /locus_tag="VFD06_11355"
     CDS             3934..5349
                     /locus_tag="VFD06_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN04167.1"
                     /translation="MKAAVIGGGVLGLTLAYRLASQGHRVDLLEAEPALGGLAAAHDY
                     GPFTWDRFYHCVLPQDTSLIALFEDLGLAGDLRWTRTGTGYYARGRMYDMNGNADFLR
                     FPLLTLTDKARLAAAVFYATRLAKPEHLYRVSAEQWLIRICGRRAYGVFWQPLLKAKF
                     GPFHDQVAAVFIYATLVRLFGARSATAGKESLGYVRGGYRRILGRFAERLRERGATLR
                     TSAPVTGIAPAPGPSGGPGCRVEWRSADAPAGDNYDQVFFTAPTRLARRVAAAPFLPH
                     VEAVERENPTSAHYLGVACLVLALEAPLTPYYVLNIGESSIELTGLIEMTNLVDRSSE
                     TANLALLYLPRYMDSESKEFEASDAALRDGMIERGLKRLFPGFDARHARYAGVHRARY
                     VQPLPLVRDGGGPQGDSTPALSMPFQIVNTAMLRCATLNNNEVVALVDRFMTKNRASL
                     APTPSTSPEPGRAPAPVRSTR"
     gene            complement(5258..6496)
                     /locus_tag="VFD06_11360"
     CDS             complement(5258..6496)
                     /locus_tag="VFD06_11360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZN04168.1"
                     /translation="MTDRWRTVLAAAGFAAISSIVAARTIANLNVPGQPTAQMFGLQD
                     FRDNFYYPSVALLDGRNPYDYADYHATYPIDRPFPAYSPISLLLHLPLGMLPYGLAET
                     GDFLLNLALMIALAWLALRGSGQEIKTWKVLTVSALLVLSRPGHMTLYIGQCSAMMAV
                     ASTVALLYGKRRPLVGALGLAAACAKPSYGGPLGVLMLLRGDGRALFVGSALAAVLGI
                     LGGLGPILRAGGIGPWIQTVRGSMQVVATDGTFNEPTSLIRLDPVSLIGRLIGHPPGT
                     LVYVALAILILGAAGLLMRRLAARGEPEPRAISNSLGCLAILLATYHQPYDALILALP
                     ASVVLLTPGRVEGRRLALLLLLLVPGLNYVATHTLLERLEVGRFAWLVISESNGLALA
                     LALVLGMWMAWGRAKRGFSS"
     gene            complement(6493..8034)
                     /locus_tag="VFD06_11365"
     CDS             complement(6493..8034)
                     /locus_tag="VFD06_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04169.1"
                     /translation="MSRSRAILLLAVVLAAAGTLVAARLGAQSLWIDEAITLVPVTAA
                     DGPADLVARVRRLDSQPPASHLLLYALRDLLPQDEVGWRLPSLLAAEIGILLLALTSG
                     RLLGPFGLLAAGVCAQASPYLLFYAMEARNYALWFLAVAASTYALTRCLEAIAAHRPA
                     REVAPWAAAWAIANGLGLWTHLFHLFALVVQAVVLAGLAWRLRPPREVARRAAAWSAA
                     AFAGSAVIVLPWLVTVLRAFGVARGVGWTRPFSAAGLAYFPFALVFGFSLGPDLRELH
                     VHGPRQLLTGHPMALGLGSVSLLFLGAGLWSLLRGERSVPAGQFQETIDAAAPVLFLV
                     APAAGLLGPLLFAEVRGFPLVPRHLMFLWPLVPLLQAYIAARSPRFRPALAAIVALQI
                     TACGSLLFDPVYAKDDERGAIRYAEARSGSRPVVLGDAAPLYTARGLGLMKAFTDPKS
                     DAVLGSGATDLWLVDNRPWEDPEGNLRTKAAQAAALLGLEPAGDHEQFRGLVLRHWRR
                     AEVAR"
     gene            complement(8031..>9415)
                     /locus_tag="VFD06_11370"
     CDS             complement(8031..>9415)
                     /locus_tag="VFD06_11370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN04170.1"
                     /translation="LWMDEYIWTLTASLTSLGAILNRPDGYPPTMALLVRGLTALGLD
                     SDFALRAPSALAGTLSVPALYFLGRRLIGHRAALAAAGLLAIHPFAVWYSQELGAYAL
                     LSFFALTSSLCLLSLIDGGGLPAALAYAVSVTCGFGVHYYFAFVVLAHFPFAVAALGR
                     ARGPARRTLLVTAALTAGGLLLWAPIFLDDVRAQREHDRGSTASLFAVPYTAQLFAGG
                     FGLGPPLRDMHAAMRAGSTPTGITMRQMAPSIAGMAILTGLILLGLGADWGGRRALLV
                     TLLILPFLGPWVNSFAGVGYRPRYAVAALPFALLLAAGGLATKQRKTALLLVVPLVLV
                     ELYGLVRSFHPEHAREDTRSAAIYVAAGGGGPVLLLGEGAEAYKRYARSRGGLLDLDL
                     KDVRDPERLGRLLDPILAGGTDVWLVSSRPWTEDPDGEVPRRLALTLRPAESKRFAGV
                     VVQRFVRPPA"
BASE COUNT         1562 a         3314 c         3355 g         1184 t
ORIGIN      
        1 tgagacgctc tacgacgagg agcggccggc gctcgtgctg gtgcaggggg acacgacgag
       61 cgcgctctgc gccgggctgg ccgccttcca tcggcgcatc gccgtcgggc acgtcgaagc
      121 ggggctccga tccttcaacc gcttccatcc gtaccccgag gaggcgaaca ggaggatgct
      181 ctcctcggtc accgacctgc acctggcggc gacgccctgg agcgccgcca atctcgagcg
      241 cgagggggtg aggcgcgagg agatcgtggt gaccggcaac accgtcatcg acgcgctgct
      301 cgaatcgctc gatcgtcccg atcggctcgg ccggcagacc gcttttccac ggcaggcggg
      361 ggagcggctg gtcctgatca ccctgcaccg ccgcgagaac tggatgcacg gcgcacaggg
      421 ggcgggcgcg cccggacgag cggtgcagga atcgccgctc gaggagacgc tcgccgccat
      481 cggtgacgcc gcgacccggt tccccgcggc ccgcttcgtc tacccggtgc acagaaatcc
      541 aagagtgcag gagccggcgc gccggatcct cggcggccgt cccaacgtgc acctgctcga
      601 cccgctgccg tacctgccgt tcgtggatct cctcggccgc gcttccctca tcctgaccga
      661 ttcgggcggg atccaggagg aggcgccgtc gctcggcgtg ccggtgctgg tggcgcggga
      721 gacgaccgag cgccccgagg gggtggaggc cgggacgaac gtcctcgtcg gcacgcgccg
      781 cgacgccgtc ctcgccgcca tgacgaagca cctcgagcgg ccgttcgagt cccccgggca
      841 gcgccctcgc cccaacccgt tcggggacgg ccaggcgagc gagcggatcc gacaggccgt
      901 cctgcaccac ctcgggctgg gagaacaagc gaccgaattc gtgcccccga tggcatcatc
      961 ctcactttca tccgccccgc gcctcgaggg cgccggcgcc cggaggccct gaatgtccga
     1021 gacgaagacg aagacgaaga tcaccctgcc ctccgacgcc gactgcagcg gccggacgtt
     1081 cggcgccgaa gagttggcga tcctccagcg cgtcatcgcc tcggggacgc tgaactgcac
     1141 caaggggacc gtggtgcggg agttcgagaa gcggttcggc gagtcgctcg gcgtgccgtt
     1201 ctgccgcacg acgacctccg gaacggccgc gctgcacgcc gcggtggcgg cgatcgaccc
     1261 ggagccgggg gacgagatca tcacgacgcc gatcaccgac atgggggcga tcacgccgat
     1321 cctgtaccag accgcgatcc cggtcttcgc cgacgtcgat ccgctgacct acaacctcac
     1381 cgccgagacg atcgcgaaga agatcacccg ccgcaccaaa gcggtcatgg tgacgcacct
     1441 gttcggcaac gcctgcgaca tggacccgat catggccctc gcccgcgagc gcaacatcaa
     1501 ggtgatcgag gactgcgccc aggccttcca ctgcacctac aaagggaagc cggtcgggac
     1561 catcggcgac ctcggctgct tcagcctgca gcagaccaag cacatgaccg ctggtgaggg
     1621 ggggatggtc gtggcgcgcg acgaggcgct gtcgcgccgc gcccgcctgt tcatcgacaa
     1681 ggcgtggggg tacggcgatc cgaagccgga ccactacttc ctggccctca actaccggat
     1741 gaccgaggtc gtgggggcgg tggcgctcgc ccagttcgac aagctcgagg cgatggtgaa
     1801 gcaccgcgtc gcgatggcgg acgcgctctc gaagaagatc gaaggggtga aggggatcgc
     1861 ggcgccgaag atcaccccgg gctgcaagcc ggtctactgg aagtacccgc tgcgcatcga
     1921 cacgaacctc gtccgcggcg gcgccgacgc cttcggcgcg cgcctcaagg aggacggggt
     1981 tttcacggcg ccgcgctaca tccagaagct ggcgttcgag tgcgaggtgc tgcgcgacca
     2041 gcgcacgttc gggaacagcc gcttcccgtt cgtcggcgaa caccgcaacg gcgacgcgcc
     2101 tgttgtttac gataagacgg acactccggg ggcggtcgaa gcgctcgccg gagtcgtcgt
     2161 gctgccctgg aacgagaagt acactccgga acacgtcgag ttcatcgccg cgtcgatccg
     2221 acaggcctcg aaagagctga cttgagcgga cgaccgtcgc acgaacgaag gaggctgccg
     2281 tggggaaagt cgggaagggg gatgcggtcc gtttcggtgt cgtcggctgc ggtcgcatcg
     2341 cgcagtcgca cctgcaggcg ctcgggacgc tttccgacgc gcggctgacc gccgcggtcg
     2401 agagccggaa gccggccggg gaggcggtgg cgcaggagta caagtgcgcc ctgtactccg
     2461 actaccgcga gccggcgctg ctcgagcaga tcgacgcggc gatcatctgc acgccgccca
     2521 gcctgcacca cgacatcgcc cggcacttcc tcgaacgggg cgtgcacgtc ctgtgcgaga
     2581 agcccctgac catcgagtcg gccgaggcgc gcgacctcgt ggcgctgtcg gacgagaagg
     2641 acctcgtgct gatgatggcg tcgaagttcc gctacgtgga cgacatcatc aaggcgaagg
     2701 cgatcatcga gtccggcatc ctcggccgca tcatcctcta cgagaacacc ttctgctcca
     2761 aggtggcgat gtccgaccgc tggaacgcca acaaggaggt ctccggcggc ggcgtgctca
     2821 tcgacaacgg ctcgcactcg gccgacatcg cgcggtacct gctcggcccg atcgcggacg
     2881 tgcaggcgca gaacggcctg ccgacccagg ggctcgacgt ggaggacacg gtgcgggtgc
     2941 agttcgtcac tcaggggggc gtcatcggga cgttcgacct ctcctggagc atcaccaaag
     3001 agacgccaca ctacgtgcag gtgttcggct ccgaaggcac ccttcaggtg ggatggagcg
     3061 gctcgaagta ccggcaggac ggctcgtccc gctgggtgcc gttcggcacc ggctacgaca
     3121 agctgaaggc gttctcccgg cagctgcaga acttcatcgg gtgcatcaag gggcgcgagg
     3181 cgccggtgat cagcgcgggg gacagcctgg cgtcggtgca ggtgatcgag accgcctacc
     3241 gatccgccgg ccgcaacaac tggctgcgcg tcgaaccggc ggcgtagtga aaccggcgcg
     3301 cgtccatccc accgccctcg tcgaggaagg ggtggtcctc ggccacgaca ccagcgtctg
     3361 ggacggggtc cacatccgcc acggcgcgac gatcggccac cactgcatcg tcggcgagaa
     3421 gacctacatc gcctacgacg tgtcgatcgg caactacgtc aagatcaacg ccgtcgtcta
     3481 catctgcgcc aacgtgtcga tcggcgactt ctgcatgatc gccgcgcaca ccgtttttgc
     3541 caacgatcgc ttcccgcgct cgggcaaccg ggagctgacc gggctcgaga cctccgacgt
     3601 gacggaggag acgctgacga cgcggctcga gcgcggcgtc accatcggcg ccaacgcgac
     3661 catcggcccc gggatcacgc tcggggagtt ctccatggtc gggatggggt cggtcgtgac
     3721 ccgcgacgtg ccggccttcg cgctggtgca cggatcgccg gcgaaggtgg ccggatggct
     3781 gtcggcctgc gggcacccgc ttctgcgcgg ccgcgacctc ccggcgccgg acgacgcggc
     3841 ggcatcccgc gacctcccct gcgagcgctg cggccgcgac tatcgcttcg acggccgcgc
     3901 gctgagtccc agggtcccga tcgtctcggc ctcatgaagg ccgcggtcat cggcggagga
     3961 gtcctcggcc tcaccctcgc gtaccggctc gcgtcgcagg ggcaccgggt cgatctcctc
     4021 gaggcggagc cggcgctcgg cgggctggcg gcggcgcacg actacggccc cttcacctgg
     4081 gaccgtttct accactgcgt gctgccccag gacacgagcc tcatcgcgct cttcgaggac
     4141 ctcggccttg cgggcgatct tcgctggacg cggaccggca ccggctacta cgcgcgcggc
     4201 cggatgtacg acatgaacgg gaacgccgac ttcctgcgct tcccgctcct gaccctcacc
     4261 gacaaggcgc gcctggcggc ggcggtgttc tacgcgacgc gcctggcgaa gccggagcat
     4321 ctgtaccgtg tcagcgccga gcagtggctc atccgcatct gcggccgtcg cgcctatggg
     4381 gtgttctggc agcccctcct caaggcgaag ttcgggccgt tccacgacca ggtggcggcg
     4441 gtcttcatct acgccacgct cgtgcgcctg ttcggcgccc gctcggccac cgccgggaag
     4501 gagagcctcg gctacgtccg cggcggctat cgccgcatcc tcgggcgctt cgccgagcgg
     4561 ctgcgggagc ggggcgcgac cctccgcacc tcggcgccgg tcaccggcat cgcaccggcg
     4621 ccgggcccgt ccggaggccc cggctgccgc gtcgagtggc gatcggccga cgcgccggct
     4681 ggcgacaatt atgatcaggt tttcttcacc gccccgacgc ggctcgcccg ccgcgtcgcc
     4741 gcggccccgt tcctgccgca cgtcgaggcg gtcgagcggg agaacccgac gtcggcccac
     4801 tacctcggcg tcgcctgcct cgttctggcg ctcgaggcgc cgctgacgcc gtactacgtc
     4861 ctcaacatcg gcgagtcgtc gatcgagttg accgggctca tcgagatgac caacctcgtg
     4921 gaccggtcga gcgagaccgc gaacctcgcg ctgctgtacc tgccgcgcta catggacagc
     4981 gagtcgaagg agttcgaggc gtccgacgcg gcgctgcgcg acggcatgat cgaacggggg
     5041 ctgaagcggc tcttcccggg tttcgacgcg cgacacgccc ggtacgccgg cgtgcaccgg
     5101 gcccgctacg ttcagccgct gccgctggtg cgcgacggcg gaggacctca gggggattcg
     5161 acgcccgccc tgtccatgcc gttccagatc gtcaacacgg cgatgctgcg ctgcgccacc
     5221 ctcaacaaca acgaggtggt ggcgctcgtc gatcggttca tgacgaaaaa ccgcgcttcg
     5281 ctcgccccca cgccatccac atccccagaa ccagggcgag cgccagcgcc agtccgttcg
     5341 actcgctgat cacgagccac gcgaaacggc ccacctcgag ccgttcgagc agcgtgtggg
     5401 tcgcgacgta gttgagcccc ggcacgagga gcagcaggag gagcgccagg cgccttccct
     5461 cgacccggcc cggcgtcaga agcaccaccg acgccggcag cgccaggatg agggcgtcgt
     5521 acggctggtg gtaggtggcg agcaggatgg cgaggcagcc gaggctgttg gagatcgcgc
     5581 gcggctccgg ctcgccgcgc gccgccagcc gccgcatcag gagcccggcc gcgccgagga
     5641 tgaggatcgc cagggccacg tacacgagcg tccccggcgg gtgcccgatc agccgcccga
     5701 tcagcgacac cgggtcgagc cggatgaggc tcgtcggctc gttgaaggtg ccgtcggtcg
     5761 cgacgacctg catgctgccg cgcacggtct ggatccacgg gccgatgccg ccggcccgga
     5821 ggatcggacc caggccgccg aggatcccga gcaccgccgc cagggcggat cccacgaaca
     5881 aggcgcgccc atccccgcgc aggagcatca ggaccccgag cggcccgccg tacgaaggct
     5941 tggcgcacgc ggccgcgagt ccgagggccc cgaccagcgg gcgccgcttt ccatagagaa
     6001 gcgcgacggt gctggcgacc gccatcatcg cgctgcactg gccgatatag agggtcatgt
     6061 ggccgggccg gctgaggacg agcagggccg acacggtgag gaccttccac gtcttgatct
     6121 cctgcccgga cccgcgcagc gccagccacg ccagcgcgat catcagcgcg aggttgagga
     6181 ggaagtcccc cgtctccgcc agcccgtacg gcagcatccc gagcggcagg tgcaggagga
     6241 ggctgatcgg agagtacgcg gggaacggcc ggtcgatcgg gtacgtggcg tggtagtcgg
     6301 cgtagtcgta ggggttgcgt ccatcgagca gggcgaccga cggatagtag aaattgtcgc
     6361 ggaagtcctg cagcccgaac atctgcgcgg tcggctggcc cgggacgttc aggttggcga
     6421 tggtgcgcgc cgcgacgatc gaggagatcg ccgcgaagcc ggccgccgcc agcacggtgc
     6481 gccagcgatc ggtcaccgag cgacctccgc gcggcgccag tggcgcagga cgaggccgcg
     6541 gaactgctcg tggtccccgg cgggctcaag accgagaagc gccgccgcct gcgccgcttt
     6601 cgtgcgcagg ttcccctcgg ggtcctccca cggccgattg tccacgagcc acaggtcggt
     6661 cgcccccgac ccgagcaccg cgtcgctctt cggatcggtg aacgctttca tcagaccgag
     6721 gccgcgcgcg gtatagagcg gcgccgcgtc tccgaggacg accggacggc tccccgagcg
     6781 cgcctcggcg tagcggatcg ccccgcgctc atcgtccttg gcgtagaccg ggtcgaagag
     6841 caggctgccg caggcggtga tctgcagcgc gacgatggcg gccagcgccg gccggaagcg
     6901 cgggcttcgc gcggcgatgt acgcctgcag gagcggcacc agcggccaca ggaacatcag
     6961 gtggcgcggg accagcggga agccgcgcac ctcggcaaac agcaacggtc ccaggagccc
     7021 ggcggccggg gcgacgagaa acaacaccgg cgccgcggcg tcgatcgttt cctggaactg
     7081 gccggccgga accgagcgct cgccgcggag aagcgaccag agacctgctc ccagaaacag
     7141 gagcgacacg gaaccgagac cgagcgccat gggatgcccc gtcaggagct ggcgcggtcc
     7201 gtggacgtgc agctcgcgca gatcgggccc gagggagaat ccgaagacga gcgcgaacgg
     7261 gaagtacgcg aggccggccg ccgaaaacgg tcgggtccat ccgactccgc gcgccacgcc
     7321 gaatgcgcgc aggaccgtca cgagccacgg caggacgatc accgccgacc cggcgaaagc
     7381 cgccgccgac caggcggccg cccggcgcgc gacctcccgc ggcggccgca gccgccacgc
     7441 caggccggcg aggacgaccg cctgcaccac gagggcgaac agatggaaga gatgcgtcca
     7501 gagcccgagg ccgttggcga tcgcccaggc cgcggcccag ggggcgacct cccgcgccgg
     7561 ccggtgcgcg gcgatcgcct cgagacagcg ggtcagagcg tacgtggagg cggcgaccgc
     7621 caggaaccac agggcgtagt tgcgcgcctc catcgcgtag aagaggagat acggcgaggc
     7681 ctgggcgcac acgcccgcgg cgagcaggcc gaacggcccg agcagccgcc ccgatgtcag
     7741 ggccagcagc aggatgccga tctccgccgc cagcagcgac ggcagccgcc agccgacctc
     7801 gtcctgcggc aggagatccc gcagtgcata aaggagcagg tgcgaggcgg ggggctgcga
     7861 gtcgagacgc cgcacgcggg cgacgagatc cgccgggcca tcggcggcgg tcaccgggac
     7921 gagcgtgatc gcctcgtcga tccacaggga ctgcgcaccg agccgcgccg ccacgagcgt
     7981 gcccgcggcc gcgagcacca cggcgaggag caggatcgcg cggctccggc tcatgcggga
     8041 ggcctcacga agcgctgcac cacgaccccg gcgaagcgct tcgactccgc cggccgcagc
     8101 gtcagggcca ggcggcgcgg cacctcgccg tccgggtctt cggtccaagg gcgcgacgac
     8161 acgagccaga cgtcggtgcc gcctgcaagg atcgggtcga gcagccggcc gagccgctcc
     8221 ggatcgcgca cgtccttgag atcgagatcg aggagtcccc cccgggagcg cgcgtagcgc
     8281 ttgtacgcct ccgccccctc gccgaggagg agcaccggcc cgccgccgcc ggccgcgacg
     8341 tagatcgctg ccgagcgggt gtcctcgcgc gcgtgctccg ggtggaagga gcgcacgagc
     8401 ccgtacagct cgacgaggac gagcggcacg accaggagca gggcggtctt ccgctgcttc
     8461 gtcgcgaggc cgccggccgc gagcaggagg gcgaacggca gggcggcgac ggcgtagcgc
     8521 ggccgatatc cgaccccggc gaacgagttc acccacggcc cgagaaacgg caggatcaga
     8581 agcgtcacga ggagcgcacg ccgcccgccc cagtcggcgc cgagccccag gaggatgaga
     8641 ccggtcagga tcgccatgcc ggcgatcgac ggcgccatct gccgcatcgt gatcccggtc
     8701 ggggtggacc cggcccgcat ggcggcgtgc atgtcgcgca gcggcggtcc gaggccgaag
     8761 ccgccggcga agagctgcgc cgtatacggc acggcgaaga gcgacgcggt ggagccgcga
     8821 tcgtgctccc gctgcgcccg gacatcgtcg aggaagatcg gcgcccagag gagaagcccc
     8881 ccggcggtca gcgccgcggt cacgagcagc gtgcggcgcg cgggcccgcg cgcccgcccg
     8941 agcgccgcga cggcgaacgg gaaatgcgcc agcaccacga aggcgaagta gtagtgcacg
     9001 ccgaacccgc acgtgaccga gacggcgtac gcgagagcgg ccggaagccc tccgccgtcg
     9061 atgagactca agaggcataa gctcgaggtc agcgcgaaga aggacagaag cgcataggcc
     9121 ccgagctcct gcgagtacca gacggcgaac gggtggatcg ccaggagccc cgccgctgcg
     9181 agggcggccc ggtgcccgat gaggcggcgg ccgagaaagt aaagggccgg caccgacagc
     9241 gtgccggcga gggccgacgg ggcgcgcagg gcgaaatcgg agtcgagccc gagcgccgtc
     9301 aggccgcgga ccagaagcgc catcgtcggc gggtacccgt ccgggcggtt gaggatggcg
     9361 ccgagcgacg tgagcgaggc ggtcagcgtc cagatgtact cgtccatcca gagcg
//
LOCUS       DASRIQ010000465         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_196882, whole genome shotgun sequence.
ACCESSION   DASRIQ010000465 DASRIQ010000000
VERSION     DASRIQ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196882"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFD06_11375"
     CDS             <1..199
                     /locus_tag="VFD06_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04171.1"
                     /translation="ACAESADLQNDGQIDISDPIYLLLHLFAGGPPPAQPGPTGSPCG
                     NDTHAPGSAGDLGCERYDPCG"
     gene            complement(216..1703)
                     /locus_tag="VFD06_11380"
     CDS             complement(216..1703)
                     /locus_tag="VFD06_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HZN04172.1"
                     /translation="MTRVAAVLIAAAVVPGAAWCGEGPLGVFEAHGDVGKVEHPGAVT
                     FDEGARRYTVTGGGENMWFAADAFHFAWKKMSGDVSLAAVIAFIGAGKNPHRKACLLI
                     RQSLDADSAYVDAALHGDGLTSLQFRESRGAATHEVQAGVSRPARLRLEKRGPYARLH
                     VAAAREEPLEFSGAAIRVSFTDPFYVGIGVCAHDPDVTEQAVFSELEIGAPPPASRPP
                     VLHSALETQAVASTDRRVVHVAPVRFEAPNWSRDGRWLIFNGEGKLHRIPSSGGKPEV
                     IDTGFATRLNNDHGVSPDGAVLAISDQSQGLRRSLIYVLPIAGGTPRQVTAQGPSYWH
                     GWSPDGKTLAFCGERDGEFDIYTIPAAGGAETRLTTAKGLDDGPEYSPDGRYIYFNSD
                     RAGRMKIWRMRAEGGGEEPVTFDEYNDWFPHPSPDGRHLVFLSYEKDVTGHPENKDVL
                     LRRMALDEKKIDVLARLLGGQGTINVPSWSPDGRRIAFVSYQLVP"
     gene            complement(1700..3121)
                     /locus_tag="VFD06_11385"
     CDS             complement(1700..3121)
                     /locus_tag="VFD06_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN04173.1"
                     /translation="MTPTQWLICFIAAVGFAFDIYELLMLPLIIKPALAALGGTAPGG
                     GPLLVPGSEEFTRWARLLFFVPALVGGTAGLVGGYLTDRLGRRRVLSGSILLYAFSAF
                     ASGFARTPMELLVLRCLVFTGVCVEFVAAVAWLAELFQEPKQREKVLGYTQAFSSFGG
                     LLVALANRGCIAAADSLPAINGGHEPWRYTLISGLLPAIPLMLIRPFLPESPVWRAKR
                     LAGTLKRPSIAQIFRPDLIRTTLTTTILFACSYGIAFGAIQHLPQIVPGLADVKARAA
                     EAREQILHEAISQGKPAPPAPVLAAAARRVEEDAAAVVQTWQEIGGLLGRLALAGLAV
                     WIVGRRLLYRVFQIPCLFFVPLFFYWLSENLGETGNNDLVKWGVLLAGFLTVSQFSFW
                     GNYLPRVYPVHLRGTGESYAANIGGRIFGTMAAWVTLTLSASTPPDPARIAWTAAAVA
                     GAVCLVGAIVTHWLPEPGEELPE"
BASE COUNT          513 a         1086 c         1130 g          427 t
ORIGIN      
        1 tgcgtgcgcc gagtccgccg atctgcagaa cgacgggcag atcgacatct cggaccccat
       61 ctacctgctc ctccacctct tcgccggggg accgccgccc gcgcagccgg ggcccacggg
      121 ctcgccctgc gggaacgata cgcacgcgcc cggctcggcc ggcgatctgg ggtgcgagcg
      181 ctacgatccg tgcgggtagg gtgagggccg ccccttcatg ggacgagctg gtagctcacg
      241 aacgcgatcc tccggccatc cggcgaccac gacggcacgt tgatcgtccc ctggcctccc
      301 aggagccggg cgagcacatc gatcttcttc tcatctagag ccatgcgccg gagcagcacg
      361 tccttgttct cggggtgacc cgtgacgtcc ttctcgtagg acaggaacac gaggtgccgg
      421 ccgtcgggag atggatgcgg gaaccagtcg ttgtactcgt cgaaggtcac cggctcctcg
      481 ccgccgccct cggcgcgcat ccgccagatc ttcatccgcc ccgcgcggtc ggagttgaag
      541 tagatgtaac gcccgtccgg agagtactcc ggaccatcgt cgagcccctt cgcggtcgtc
      601 agtcgcgtct ccgcgccgcc cgccgccggg atcgtgtaga tgtcgaactc gccgtccctc
      661 tcgccgcaga aggcgagcgt cttcccgtcg ggcgaccacc cgtgccagta ggaggggccc
      721 tgcgccgtca cctgccgcgg cgttccgccg gcgatgggga gaacgtagat cagggaccgg
      781 cggagcccct gcgactggtc gctgatggcg aggaccgctc cgtccggcga gaccccgtgg
      841 tcgttgttga gccgcgttgc gaaccccgtg tcgatgacct cgggcttgcc gcccgaggac
      901 gggatgcggt ggagcttccc ctcgccgttg aagatcagcc accggccgtc gcgcgaccag
      961 ttgggcgcct cgaagcgcac gggggcgacg tggaccacgc ggcggtccgt ggacgcgacg
     1021 gcctgcgtct cgagcgcgct gtggagcaca ggcggccggg atgcgggcgg cggagcgccg
     1081 atctcgagct ccgagaacac cgcctgctcc gtcacatccg ggtcgtgagc gcacacgccg
     1141 atgcccacgt agaacgggtc cgtgaacgac acgcggatcg ccgcgcccga gaactcgagc
     1201 ggctcctcgc gcgcggccgc gacatggagc cgcgcgtagg ggccgcgttt ctcgagcctc
     1261 agccgcgcgg ggcgcgagac gccggcctgc acctcgtgcg tcgcggcgcc cctggactcg
     1321 cggaactgga gcgaggtcag cccatcgccg tggagggctg cgtcgacgta ggccgagtcc
     1381 gcatcgaggc tctggcggat gaggagacag gccttgcggt gcgggttctt ccccgctccg
     1441 atgaacgcga tgacggccgc cagggaaacg tccccggaca tcttcttcca cgcgaagtgg
     1501 aaggcgtcgg ccgcgaacca catgttctcg ccgccgccgg tcacggtgta gcgccgcgcg
     1561 ccctcgtcga acgtgaccgc accgggatgc tcgaccttcc ccacgtcgcc gtgagcctcg
     1621 aataccccga ggggaccctc tccgcaccag gccgctcccg gcaccaccgc ggcggcgatg
     1681 aggacggcgg cgacccgcgt cactcgggga gctcctcacc cggctccggc agccagtggg
     1741 tcacgatggc gcccaccagg cagacggcgc ccgcgaccgc ggccgccgtc caggctatgc
     1801 gagctggatc cggcggcgtg gaggcggaca gggtcagcgt cacccaggcg gccatggtgc
     1861 cgaagatgcg gcccccaatg ttggcggcgt aggactcgcc ggtgccccga aggtggaccg
     1921 ggtacaccct ggggaggtag ttcccccaga acgagaactg ggacacggtc aggaatcccg
     1981 cgagcaggac cccccacttc acgaggtcgt tgttcccggt ctcgccgagg ttctcgctga
     2041 gccagtaaaa gaaaaggggg acgaagaaga ggcaggggat ctggaagact cggtagagga
     2101 gcctccggcc gacgatccag acggcgagcc ccgcgagagc gagcctgccg agcaggcccc
     2161 cgatctcctg ccatgtctgc acgacggctg cggcgtcctc ctcgacgcgg cgcgcagcgg
     2221 cggcaaggac cggagcgggc ggtgccggct tcccctgcga gatggcctcg tgcaggatct
     2281 gctccctcgc ctccgccgca cgcgccttca cgtccgcgag gccggggacg atctgcggca
     2341 ggtgctggat cgcgccgaag gcgatgccgt aggagcaagc gaagaggatg gtggtcgtca
     2401 acgtcgtccg gatcaggtcc ggccggaaga tctgggcgat gctcgggcgc ttgagcgtcc
     2461 ccgcgaggcg cttcgcccgc cacaccggag attccggcag gaagggccgg atgagcatca
     2521 acgggatggc ggggaggagg cccgagatca gcgtgtagcg ccacggctcg tggccgccgt
     2581 tgatcgccgg gagggaatcg gcggccgcga tgcagcccct gttcgcgagc gccacgagaa
     2641 ggccgccgaa cgacgagaac gcctgcgtgt aaccgagcac cttctcacgc tgcttcggtt
     2701 cctggaagag ctcggcgagc caggcgaccg ccgccacgaa ctccacgcag actccggtga
     2761 agacgaggca gcggagcacg aggagctcca tgggcgtcct cgcgaagccc gacgcaaagg
     2821 cggagaaggc gtagaggaga atgcttccgg agaggacgcg ccggcggccg agccggtccg
     2881 tgaggtagcc gccgacgagg ccggcggtac ctccgaccag agcgggcacg aagaagagga
     2941 gccgcgccca ccgcgtgaac tcctcggatc ccgggacgag gagcggtccg ccgcccggag
     3001 ccgtcccccc cagcgcggcc aaggccggct tgatgatgag gggcagcatg aggagctcat
     3061 agatgtcgaa ggcgaagcct acggcggcga tgaagcagat cagccactgg gtcggggtca
     3121 agcggggctc ccgctggggc tcgtcgaggt caaggc
//
LOCUS       DASRIQ010000466         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2008527, whole genome shotgun sequence.
ACCESSION   DASRIQ010000466 DASRIQ010000000
VERSION     DASRIQ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008527"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFD06_11390"
     CDS             <1..651
                     /locus_tag="VFD06_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZN04174.1"
                     /translation="LAIGAHPDDIEFGCGGTLIKYARKEAHIELLVMTDGARGGSPKV
                     RRREQKEAAAVLGASRVHWGGYRDTLLPSVRHLIDRMETILHGARPDFIFVNSPEDTH
                     QDHRLVARAAISATRWARNVLFYEGPTTVNFTPTVFIDIAAEIDRKVEALHRHQSQIG
                     KTRIEGTPISEIAQASAHFRGVQGRVRWAEGFAPLRLFINVTGHPEARSDTKRKAA"
     gene            648..1772
                     /locus_tag="VFD06_11395"
     CDS             648..1772
                     /locus_tag="VFD06_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HZN04175.1"
                     /translation="MKSTAALRRTPAPADASIGRASGFVPHARPAIGTDEEEAALRVL
                     RSARLAPGSEAARLEGLLSRLAGAADAVALQSGTLSLTLALRALRAGPGDRVAIPAYG
                     CAALLHAVHAAGCVPVIADIDPDTLALDPDSLRRRADPHGLRAVVVVHPFGMPVPVEP
                     YRAAGRIVIEDCAQSPGARLGLSPVGSFGDAAVFSFGPTKLLTCGGPGGGIASGDPAI
                     VRAARDLARHDEKEDAAPRLNGLMGDLHAAIAAAQIGRLHEIVARRRAIAERYDAAFG
                     DLALRPRLAGGARPVFHRYLLSVPARASALIDLLGRRGLQARTPVHRPLHRLVAGYTP
                     CPGADEAHARWVSLPLSAAFTETEVDRVIGEVQACLSPRS"
     gene            1751..2863
                     /locus_tag="VFD06_11400"
     CDS             1751..2863
                     /locus_tag="VFD06_11400"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZN04176.1"
                     /translation="MPVAALLTAAIWFLLRPTAAGPAEIAAAAFLAAYGLTPGARLMA
                     LGAGALDHPEARRSHLQPTPRLGGVAVIAAFLLVLGRRALDDRELLAIVLASLALMTA
                     GALDDTRGLQARWRLLLQVGCALFVMAAGVRLHLLPGETGRMIDGLLTLVWIVGLTNA
                     YNFIDGIDGLAASLGALIAVLLGVSAAAGGQAAMMPAAAALAGALLGFLPHNLRTRGR
                     PASIFLGDSGSASVGFVLAALAIKEDWAEGDPLAALATPILIFSVLIYDMVQTTLARI
                     TSGKVRTFRQWIDYTGRDHIHHRFSAVLGGSRRALALILTLALGVGLSALGLRHAGTG
                     LALLLLLHAALVLLVVEILIGAARRRASASPDEERA"
     gene            2860..>3695
                     /locus_tag="VFD06_11405"
     CDS             2860..>3695
                     /locus_tag="VFD06_11405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN04177.1"
                     /translation="MRDRAPGPRGAAALLAAFDFPPTLGGESSLYFGLARHLPPEEIV
                     VWAPRHPGDAAIDARLRCTVARARVPAHGGAARRVARGVLAGLHLMRLMRRLPVRYLL
                     CGQVLSLGVPVRLLARAAGLRYGVFVHGADVFDFRERFPWAGLIGWVLSGADAVIVNS
                     RFTAGLLTRDYPGAARRIVVMPMGVDPAPPVPESDVAALRARYRLGDGPILLTVARLV
                     EAKGHDVVLDALPRILERVPSTRYLVVGDGPNRRALEERAGRLGVADKVVFAGRVPDA
                     EMA"
BASE COUNT          405 a         1423 c         1327 g          540 t
ORIGIN      
        1 ctggcgatcg gcgcccaccc ggacgacatc gagttcggct gcggcgggac cctcatcaag
       61 tacgcgcgca aggaggccca catcgagctc ctggtgatga ccgacggggc gcgcggcggc
      121 tcccccaagg tccgtcgccg cgagcagaag gaggcggccg cggtgctcgg cgcctcgcgc
      181 gtccactggg ggggctaccg ggacacgctc ctgccgtccg tgcgccacct catcgaccgg
      241 atggagacca tcctccacgg cgcccgcccc gacttcatct tcgtcaactc cccggaggac
      301 acgcaccagg atcaccgcct cgtggcccgc gccgccatct cggccacgcg ctgggcgcgc
      361 aacgtcctct tctacgaggg gccgacgacg gtgaacttca cgccgaccgt cttcatcgac
      421 atcgccgccg agatcgatcg caaggtcgag gcgctgcacc gccaccagag ccagattggc
      481 aagacccgca tcgaggggac gccgatcagc gagatcgccc aggcctccgc gcacttccgc
      541 ggcgtgcagg ggcgcgtgcg ctgggcggag gggttcgcgc cgctgcgtct gttcatcaac
      601 gtcaccggcc atcccgaggc gcgctccgac acgaaacgga aggccgcgtg aaatcgaccg
      661 ccgctctccg ccgcacgccc gcaccggccg acgcatcgat tgggcgcgcc tccgggttcg
      721 tgccgcacgc ccgccccgcg atcggcaccg acgaggagga ggcagccctc cgggtgctgc
      781 gctccgcgcg gctcgccccg gggtccgagg cggcgcggct cgaggggctt ctgagccggc
      841 tcgccggtgc cgccgatgcc gtggcgctcc agtccgggac gttgtccctg accctggcgc
      901 tgcgggccct tcgggccgga ccgggggatc gggtcgccat cccggcctat ggctgcgccg
      961 ccctgctcca cgcggtccat gccgcggggt gcgtgccggt gatcgccgat atcgaccccg
     1021 atacgctggc gctcgacccg gactctctgc ggcgccgcgc cgacccgcac ggactgcgcg
     1081 cggtggtcgt cgtgcacccg ttcggcatgc cggtgccggt cgagccgtac cgggccgcgg
     1141 ggcggatcgt catcgaggac tgcgcccagt cccccggcgc ccgtctcggg ctgtcgcccg
     1201 tgggctcgtt cggagacgcc gccgtgttct ccttcggacc gaccaagctc ctcacgtgcg
     1261 gcggccccgg cggggggatc gccagcggcg acccggcgat cgtgcgcgcg gcgcgcgacc
     1321 tcgcccggca cgacgagaag gaggatgccg caccgcgcct caacggtctc atgggggatc
     1381 tccacgccgc catcgccgcc gcgcagatcg gacgcctcca cgagatcgtc gcccgccgcc
     1441 gcgcgatcgc cgagcgctac gacgccgcgt tcggcgacct cgcgctccgg ccgcgcctgg
     1501 cgggcggcgc ccgaccggtg ttccatcggt atcttctctc ggtgccggcc cgcgcctcgg
     1561 ccctcatcga tctcctcggc cgccgcggcc tccaggcccg gacgccggtg caccggccgc
     1621 tgcaccgtct ggtcgcgggc tacacgccct gtcccggcgc cgacgaggcg cacgcgcgat
     1681 gggtttccct gccgttgtcg gccgccttca cggagaccga ggtggaccgc gtcatcggcg
     1741 aggttcaggc atgcctgtcg ccgcgctcct gaccgcggcg atctggttcc tgctccgccc
     1801 cacggccgcg ggcccggcgg agatcgccgc cgcggcattt ctcgccgcct acggcctcac
     1861 ccccggggcg cgcctgatgg cgctcggcgc cggggcgctc gatcatccgg aggcgcgccg
     1921 ctcgcatctc cagccgacgc cgcgcctcgg cggcgtcgcg gtgatcgcgg ccttcctgct
     1981 cgtcctcggc cggcgcgctc tcgacgaccg cgagctcctg gcgatcgtcc tcgcgtcgct
     2041 cgccctgatg accgccggcg ccctcgacga cacgcgggga ctccaggcgc gctggcgcct
     2101 cctcctgcag gtcgggtgcg ccctgttcgt gatggcggcg ggggtgcggc tgcacctcct
     2161 gcccggggag acgggacgga tgatcgacgg cctgctgacc cttgtctgga tcgtcgggct
     2221 gaccaacgcc tacaacttca tcgacgggat cgacgggctc gcggccagcc tgggggccct
     2281 catcgccgtc ctgctcggag tgtcggcggc ggccggcggc caggcggcca tgatgccggc
     2341 cgcggcggcg ctcgcggggg cgctcctggg cttcctgcct cacaatctga ggacgcgcgg
     2401 ccggccggcc tccatcttcc tcggggactc cggcagcgcc agcgtcggct tcgtcctcgc
     2461 ggcgctggcg atcaaggagg actgggccga gggtgatccg ctcgcggccc tcgcgacgcc
     2521 gatcctgatc ttcagcgtcc tgatctacga catggtgcag accaccctcg cccgcattac
     2581 gtccggaaag gtccggacct tccggcagtg gatcgactac acggggcgcg accacatcca
     2641 tcaccgcttc tccgccgtgc tcggagggag ccggcgggcg ctggcgctca tcctgaccct
     2701 ggcgctcggc gtcgggctct cggcgctcgg gttgcgccac gcggggaccg gcctggcgct
     2761 gctgctcctg ctgcacgcgg cgctggtgct gctcgtcgtc gagatcctga tcggcgcggc
     2821 gcggcgccgc gcctccgcct cccccgacga ggagcgggca tgagggatcg cgcgcccggc
     2881 ccgcgggggg cggcggcgct cctggcggcg ttcgatttcc ccccgacgct cggcggcgag
     2941 tcgagcctgt acttcgggct ggcgcgtcat cttccgccag aggagatcgt cgtctgggcg
     3001 ccgcggcatc cgggagacgc ggcgatcgac gcgcgcctgc ggtgcacggt ggcgcgcgcg
     3061 cgcgtcccgg cgcacggcgg cgccgcccgg cgcgtggccc gcggggtgct ggcggggctt
     3121 cacctgatgc gcctgatgcg gcgcctcccg gtccggtacc tcctgtgcgg ccaggtcctg
     3181 tcgctcgggg ttccggtgcg cctgctggcg cgcgccgcgg gactccggta cggcgtgttc
     3241 gtccacggcg ccgatgtctt cgatttccgg gaacggttcc cgtgggccgg actcatcggg
     3301 tgggtcctgt ccggcgccga cgcggtcatc gtcaacagcc gcttcaccgc gggactcctg
     3361 acgcgggact acccgggggc ggcgcgtcgc atcgtcgtca tgccgatggg ggtcgatccg
     3421 gcgccgccgg tgccggagtc cgacgtcgcg gcgctgcggg cgcgctaccg cctcggcgac
     3481 ggaccgatcc tcctgaccgt ggcacggctg gtcgaggcga aggggcacga cgtcgtcctc
     3541 gacgccctgc cgcgcatcct cgaacgcgtc ccgtccaccc gctacctcgt cgtgggggac
     3601 ggccccaacc gccgggcgct ggaagagcgg gccgggcgcc tcggcgtcgc cgacaaggtc
     3661 gtcttcgcgg gacgcgtgcc ggacgcggag atggc
//
LOCUS       DASRIQ010000467         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_428024, whole genome shotgun sequence.
ACCESSION   DASRIQ010000467 DASRIQ010000000
VERSION     DASRIQ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428024"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VFD06_11410"
     CDS             <1..1136
                     /locus_tag="VFD06_11410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN04178.1"
                     /translation="PAPWTQECPRSLRLEPGALHVPAATLRVLLKAGPRLNLRYAGFG
                     FEPVVRHGDLVIAAPAPPVPGALAVCDWDGYADLLRLDRVAGGWTGSIDGLPGARRSL
                     HENAILALVTPEGRAGPRPAGGDRVRLALRWPAARWFWRRIANAPSFGAAADDSVREK
                     YDRQVADYLRMQSSNLTEEQIGALGRHVRPPGRVLVAGAGSGAEVIHLARGGYRVTGF
                     DVLPAMVDASRRALDDAGVEAEILAASLDEFDPGDRRFGAIYFTPLLYSFLAGRDRRI
                     AMLRRLAAFLDPGGGLMLSEARPRGPIRRTQIGLAWLRRRLRGDRFVERGDWYTMYLA
                     PDGSIGFSFLHLFPSGQVEAELHAAGYRSVRRLGGHVIATLSP"
     gene            complement(1126..2061)
                     /locus_tag="VFD06_11415"
     CDS             complement(1126..2061)
                     /locus_tag="VFD06_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04179.1"
                     /translation="MNPPVLSPPDAPLPHAAFAAYRQPAGPDSLRIDIQELSVRLDGL
                     PSDLAAAMRGAYGPYVAGVESRPPLLRIDVMRDDRDYFIEPRFHKEWEVYRVLTEHDG
                     AIFRLTSYRFAAWYDLKLGYGQLALAQGDLDPAPRAMENFLRSAFAWLSLDRGGFFLH
                     GASIERDGRCHVFYGPSGAGKSTLSAMSRQGRVISDDLTPLLRTPQGLDALGSPFRGT
                     YRVGEPVVGRFPVAAFYRLRKDARTFLRRGDGACFADLLGNLPWVVDQLPRHPHHADR
                     VHEAVADVPFYYLHFEKDVDFWPAIDRGPDRALTD"
     gene            2129..>3109
                     /locus_tag="VFD06_11420"
     CDS             2129..>3109
                     /locus_tag="VFD06_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZN04180.1"
                     /translation="MPASAPFRLVSEFEPKGDQPEAIRRLVEGLKSGRKHQVLLGVTG
                     SGKTFTMAKAIEAVGRTALVLSHNKTLAAQLFQEFRRFFPDNAVEYFVSYYDYYQPEA
                     YVPQSDTYIEKEATINDEIDRLRHSATRSLFERRDVIIVASVSCIYGLGSPEAYHGMM
                     VLLEPGKAIDRDAMLRKLVEIQYERTPFDLERGKFRVRGDVVEIFPSYEEFAVRVELF
                     GDDIESVSAIDPVRGAVMRKLDRIPIYPNSHYVTPRDRLLKAIEAIKAELKDRLAVLE
                     SEKKLLEAQRLHQRTMFDLEMIKEIGYCQGIENYSRHLTGRQPGEPPPTLL"
BASE COUNT          481 a         1060 c         1112 g          456 t
ORIGIN      
        1 gtccggcgcc gtggacccag gagtgccccc ggtcgctccg gctcgagccg ggggcgctgc
       61 atgtgcccgc ggccaccctg cgggtactcc tgaaagccgg tccgcgcctg aacctgcgat
      121 acgcaggatt cgggttcgag ccggtcgtcc gtcacggtga cctggtgatc gccgcaccgg
      181 cgccgcccgt tccaggggcg ctcgccgtct gcgactggga cgggtacgcc gacctgctgc
      241 gactcgaccg tgtcgccggg ggatggaccg gttcgatcga cggtcttccc ggagcgcggc
      301 gctcacttca tgagaacgcg attctcgcgc tcgtcacgcc cgaggggaga gccgggccgc
      361 ggccggccgg cggggaccgg gtgcgcctgg cgctccgctg gccggcggca cgctggttct
      421 ggaggcggat cgcgaacgct ccgagcttcg gcgccgccgc cgatgattcg gtgcgggaaa
      481 agtacgaccg gcaggtcgcg gactacctgc gcatgcaatc gagcaacctg accgaggagc
      541 agatcggcgc gctcggaagg cacgtccggc cgcccgggcg ggtcctggtc gccggagcag
      601 gctcgggggc cgaggtgatt cacctcgcgc gcggggggta ccgggtcacc ggattcgacg
      661 tcctacccgc gatggtggac gcctcccggc gcgccctgga cgacgccggc gtggaggcgg
      721 agatcctcgc ggcctcgctc gacgagttcg atccggggga ccggcggttc ggcgcgatct
      781 acttcactcc gctgctgtac tcgttcctcg cgggacggga ccgccgcatc gcgatgctgc
      841 ggcgcctcgc ggcgttcctc gacccgggcg ggggcctgat gctgagcgag gcgcggccgc
      901 gtggccccat ccgccggacg cagatcggac tcgcgtggct caggcgtcgc ctgagagggg
      961 atcgcttcgt cgagcggggg gactggtaca cgatgtacct ggcgccggac ggatcgatcg
     1021 gcttctcctt cctgcacctt ttcccctccg ggcaggtcga ggcggagctg catgcggccg
     1081 gttaccgctc cgtccgccgg ctcggcggac acgtcatcgc gaccctcagt ccgtgagggc
     1141 gcgatcggga ccgcggtcga tcgccggcca gaagtcgacg tccttctcga agtgcaggta
     1201 gtagaagggg acatccgcga ccgcctcgtg cacccggtcg gcgtggtgcg gatgccgcgg
     1261 gagttgatcc acgacccagg ggagattccc caggaggtcc gcgaagcagg cgccgtcgcc
     1321 gcggcgcagg aaggtccggg catccttgcg cagacgatag aaagcggcca ccgggaatcg
     1381 ccccaccacc ggctccccga cgcgatacgt cccgcggaac gggctgccga gcgcgtcgag
     1441 cccctgcggc gtccgcagca ggggagtgag gtcgtcgctg atgacccgcc cctgccggct
     1501 catcgccgac agcgtggact tgccggcgcc cgacggcccg tagaacacgt ggcagcgacc
     1561 gtcccgctcg atgctcgccc cgtgcaggaa gaaaccgccg cggtcgagcg acagccacgc
     1621 gaaggcgctg cgcaggaagt tctccatggc gcgcggggcc ggatcgagat ccccctgcgc
     1681 gagcgccagc tgaccgtacc cgagcttcag gtcgtaccag gcggcgaagc ggtacgaggt
     1741 cagccggaat atggcgccgt cgtgctcggt caggacgcgg tacacctccc attccttgtg
     1801 gaaccgcggc tcgatgaagt agtcgcgatc gtcccgcatc acgtcgatgc gcagcagggg
     1861 cggcctggat tcgacaccgg ccacgtacgg accgtaggcg ccgcgcatcg cggccgcgag
     1921 gtcggacggg aggccgtcga gccggacgct cagctcctgg atgtcgatgc ggagcgagtc
     1981 gggacccgcc ggctgccggt acgcagcgaa ggctgcgtgg ggcaggggcg cgtcgggcgg
     2041 cgacaggacc ggcgggttca tcgaagtgtt ccgggcaggg tagcaccgag cgcgtcgttt
     2101 atgatcgagg cggagagccc gcgttaccat gcccgcgtcc gccccgttcc ggctggtcag
     2161 cgaattcgag cccaaaggag accagcccga ggccatccgc cggctggtcg aggggctgaa
     2221 gtccggacgc aagcaccagg tcctgctggg cgtgaccggc tccggcaaga ccttcaccat
     2281 ggcgaaggcg atcgaggcgg tcggccggac ggccctcgtc ctgtcgcaca acaagacgct
     2341 ggcggcgcag ctcttccagg aattccggcg cttcttcccg gacaacgcgg tcgagtactt
     2401 cgtctcctac tacgactact accagccgga ggcgtacgtc ccgcagagcg acacctacat
     2461 cgagaaggag gcgacgatca acgacgagat cgaccgcctg cgccactccg cgacccggtc
     2521 cctcttcgag cggcgcgacg tcatcatcgt cgcctcggtg tcgtgcatct acggcctcgg
     2581 ctcgccggag gcgtaccacg ggatgatggt cctgctcgag ccggggaagg cgatcgaccg
     2641 ggacgccatg ctccgcaagc tggtcgagat ccagtacgag cggacgccgt tcgatctcga
     2701 acgcggcaag ttccgggtgc gcggcgacgt cgtggagatc ttcccctcgt acgaggagtt
     2761 cgcggtgcgc gtcgagctgt tcggcgacga catcgagagt gtctcggcca tcgacccggt
     2821 gcgcggcgcg gtgatgcgga agctcgaccg cattccgatc tacccgaact cgcactacgt
     2881 gacgccccgc gatcggctcc tcaaggcgat cgaggcgatc aaggcggagc tgaaggatcg
     2941 cctcgccgtc ctcgaatcgg agaagaagct cctcgaagcg cagcggctgc accagcggac
     3001 catgttcgat ctcgagatga tcaaagagat cggctactgc caggggatcg agaactactc
     3061 gcggcacctg accggccgcc agccggggga gccgccgccg acgctgctc
//
LOCUS       DASRIQ010000468         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2515792, whole genome shotgun sequence.
ACCESSION   DASRIQ010000468 DASRIQ010000000
VERSION     DASRIQ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2515792"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..495)
                     /locus_tag="VFD06_11425"
     CDS             complement(91..495)
                     /locus_tag="VFD06_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04181.1"
                     /translation="MTQVETAPPPRSMLRSVRRALLLMVVLALLGLVLYTWVAWSFTY
                     SEGERAGYVQKFSKKGWICKTWEGELAMANLPGAMPELFRFTVHDDAVAAEINQAMGE
                     RVSIHYEQHLGLPTSCFGDTSHFVVQVRPVTP"
     gene            552..1115
                     /locus_tag="VFD06_11430"
     CDS             552..1115
                     /locus_tag="VFD06_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HZN04182.1"
                     /translation="MSVLTSVAVGIGLAAACGFRIFVPFLVLGIAARLGEVPLTSGFA
                     WLASNEALLALTVATVLEAAAYKVPWLDHLLDLLASPAAVVAGMLASAAVVTNLPPLL
                     RYATIVIGGGGAAALVQGATVLARVKSAVTTGGLGNPLVALWELGSAFVTSALAILAP
                     FLALLLLALLVGGILILIVSRSKGVRA"
     gene            1112..1504
                     /locus_tag="VFD06_11435"
     CDS             1112..1504
                     /locus_tag="VFD06_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN04183.1"
                     /translation="MIDHIVVNVRDLKASRRFYDQALAPLGYRLIKEFPGGIGYGMGA
                     KADFWMMQRAPNSEAVHVAFACGTRSLVDAFHAAALRAGGKDFGVPGVRTHYHVDYYG
                     AFVLDPDGNNIEAVCHDSAPPSGKRVRT"
     gene            1501..2535
                     /locus_tag="VFD06_11440"
     CDS             1501..2535
                     /locus_tag="VFD06_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZN04184.1"
                     /translation="MTRPSPPRSGRAATPPVGFAWDDRCLLHDNGPGHPERPQRLIAI
                     RDRLERGGTLGRLLVIAPDPAPIEALARVHEAAYVQAIRRVCERAPVRLDPDTSVSEG
                     SWEAALLSAGGGLAAVDAVLAGRARAAFVATRPPGHHAERDVAMGFCLFNSIAVAARH
                     AQERHGASRVAIVDWDVHHGNGTQHMFEEDDTVLYVSTHQWPFYPGTGARGERGRGRG
                     LGFTLNLPLPAGAGDAEYRRVFEAEVIPALERFRPDLILLSAGFDAHERDPLAGMALT
                     TGGYGRLTGLLRDAAERLCAGRIVSLLEGGYDLEALAASVETHLRVLMDEPASKPPSP
                     ATGSRRGTRR"
     gene            complement(2468..>3197)
                     /locus_tag="VFD06_11445"
     CDS             complement(2468..>3197)
                     /locus_tag="VFD06_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN04185.1"
                     /translation="WWMARIPVSPTASGSEPEEVVLLHGLGRTWRSMRPVARALERAG
                     FVTYNIGYPSLRHRLPALAAIVEERIARARQARHGAAPVVARRDAGDVPLHFVGHSLG
                     CILIRYLLAHRGLRAGRVVMLTPPNQGARLADFLQPYLSWLLRPLPDLSVGGGIPAGL
                     ACPKDVEIGLIAAGRDRTVKRHETRLEGAADSAVVPSGHSFIMMRRDAQDLTVRFLRT
                     GRFSDESPSGTRSQGSGASKPARP"
BASE COUNT          437 a         1126 c         1165 g          469 t
ORIGIN      
        1 aagcgggtag attacaggcc ctgcgggtcc tgactgtcaa gtgggttcgg gcgaatgcgg
       61 agtgagattc ggtctcgacg aaaagacccg tcagggggtg acgggccgga cctggacgac
      121 gaaatgcgac gtatctccga agcaggaggt cggcagtccg agatgctgct cgtagtggat
      181 cgacacccgc tctcccatcg cctggttgat ctcggccgcg accgcgtcgt cgtggacggt
      241 gaagcggaac agctccggca tcgctccggg aaggttggcc atcgccaact ccccttccca
      301 ggtcttgcag atccatccct tcttcgagaa cttctgcacg tagccggccc gttccccctc
      361 ggagtaggtg aagctccagg cgacccaggt gtagaggacc aggccgagaa gcgcgaggac
      421 gaccatcagg aggagcgccc ggcgcacgct tcggagcatc gatcgcggcg gcggggcggt
      481 ctcgacctgg gtcatcggcg cgcatcagac cacaaccggg gcggcgccgc aacgccgtgc
      541 gggggggcgc ggtgagcgtc ctgacgagcg tggcggtcgg catcggcctg gcggccgcct
      601 gcggcttccg gatcttcgtg ccgttcctcg tcctcgggat cgcggcgcgg ctcggggaag
      661 tgccgctcac gtccggcttc gcgtggctgg cctcgaacga ggcgctgctc gcgctgaccg
      721 tcgccaccgt gctggaggcg gccgcctaca aggtgccgtg gctcgatcac ctgctcgacc
      781 tgctcgcttc tcccgccgcg gtggtcgccg gcatgctggc gtcggcggcg gtggtcacca
      841 acctgccgcc actcctgcgc tacgccacca tcgtcatcgg cgggggtggc gcggcggcgc
      901 tggtccaggg ggcgaccgtg ctcgcgcggg tgaaatcggc ggtgacgacc ggcggcctgg
      961 ggaatccgct ggtcgcgctg tgggagctgg gatccgcctt cgtcacgtcc gcgctggcga
     1021 tcctggcgcc gttcctggcg ctgctgctcc tcgctctgct cgtaggcgga atcctcatcc
     1081 tgatcgtctc gcggtcgaag ggggtgcgcg cgtgatcgac cacatcgtcg tcaacgtccg
     1141 ggacttgaag gcgagccgcc gcttctacga ccaggcgctg gcgccgctcg gttaccgcct
     1201 gatcaaggaa ttcccgggcg gcatcggcta cgggatgggg gcgaaggccg acttctggat
     1261 gatgcagcgc gcgccgaact cggaggcggt gcacgtcgcg ttcgcctgcg gcacgcggtc
     1321 tctggtggac gccttccacg ccgccgccct gcgcgccggc gggaaggact tcggggtgcc
     1381 gggcgtgcgg acccactacc acgtcgacta ctacggcgcc ttcgtcctcg atccggacgg
     1441 gaacaacatc gaggcggtct gccacgattc ggcgccgccg tcggggaagc gcgtccggac
     1501 gtgactcggc cgtcgccgcc gcgttccgga cgagccgcca cccctccggt cgggttcgcg
     1561 tgggacgacc ggtgcctcct gcacgacaac ggcccggggc acccggagcg cccgcagcga
     1621 ctgattgcca tccgcgatcg gctggagcgc ggcgggacgc tcggtcgtct cctggtgatc
     1681 gcgcccgatc cggcgccgat cgaggcactg gcgcgcgtgc acgaggccgc gtacgtccag
     1741 gcgatccggc gcgtctgcga acgggcgccg gtgcgcctcg accccgacac ctccgtctcc
     1801 gagggatcgt gggaggcggc gctcctctcg gccgggggcg gtctcgcggc ggtcgacgcc
     1861 gttctcgcgg gccgggcgcg cgccgccttc gtcgccacgc gcccgccggg tcatcacgcc
     1921 gagcgcgacg tggcgatggg attctgcctc ttcaacagta tcgcggtggc ggcccgccac
     1981 gcccaggaac ggcacggcgc ctcgcgcgtc gcgatcgtcg actgggacgt gcatcacggc
     2041 aacggcaccc agcacatgtt cgaggaggac gacacggtgc tgtacgtgag cacgcaccag
     2101 tggccgttct acccggggac cggcgcgcgc ggcgaacggg gccgggggcg cggtctcggc
     2161 ttcaccctga acctgccgct tccggcgggg gcgggggacg ccgaataccg gcgggtcttc
     2221 gaggcggagg tgatcccggc gctcgagcgc ttccgccctg acctcatcct cctctccgcc
     2281 gggttcgacg cgcacgagcg cgatccgctg gccgggatgg cgctgacgac cgggggttac
     2341 ggccggctca ccgggctgct gcgcgacgcg gcggaaaggc tctgcgccgg ccgtatcgtg
     2401 tcgctcctgg aggggggcta cgatctcgaa gcgctcgccg cctcggtcga gacgcacctg
     2461 cgcgtcctca tggacgagcc ggcttcgaag cccccgagcc ctgcgaccgg gtcccggagg
     2521 gggactcgtc gctgaagcgg ccggtgcgca ggaagcgcac cgtcagatcc tgcgcgtcgc
     2581 gccgcatcat gatgaacgag tggccgctgg ggacgacggc cgaatcggcg gccccttcga
     2641 gccgggtctc gtgccgcttc accgtccggt cccttcccgc cgcaatgagg ccgatctcga
     2701 catccttcgg gcacgccagc ccggcgggaa tcccgccgcc gaccgaaagg tcaggaagcg
     2761 gccgcaacag ccaggagaga tacggctgga ggaagtcggc gaggcgcgcc ccctggttcg
     2821 gcggcgtcag catgaccacc cggccggcgc gcaggccgcg gtgggccagc aggtaacgga
     2881 tcaggatgca gcccaggctg tgaccgacga agtggagcgg cacgtcgccc gcatcgcgcc
     2941 gcgcgacgac gggcgccgct ccgtgccgcg cctggcgggc gcgggcgatc cgctcctcca
     3001 cgatcgccgc gagcgccggc agccggtgcc gcaggctcgg gtagccgatg ttgtaggtga
     3061 cgaagccggc ccgctcgagg gcgcgcgcca ccggccgcat cgagcgccag gtgcgcccga
     3121 gaccgtgcag cagcacgacc tcctccggct cagaaccgga cgctgtaggc gacaccggga
     3181 tcctcgccat ccaccat
//
LOCUS       DASRIQ010000469         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1241242, whole genome shotgun sequence.
ACCESSION   DASRIQ010000469 DASRIQ010000000
VERSION     DASRIQ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241242"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2803)
                     /locus_tag="VFD06_11450"
     CDS             complement(<1..2803)
                     /locus_tag="VFD06_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04186.1"
                     /translation="MNAVTQAFVTGLEEKRLFAKGTWQATQNTRLVFSVNYDPQEFTN
                     QGLNSLTRQEAGYTLKAGGPLYSARGTTVLGPSAALETTVSYLDGRPAVVPNLPLDNN
                     GDGLVSTDFNGDGFRDARERDAGEDYDGDGRFDIFEDTIARDGVLSIGEAPYCFVDGL
                     WRPTPPYSCPFPTTFLDDEDGYQIRDNWLTHSKGDKDRRLTPPGGCEGDGREDRDCDG
                     RLDTRNEDRNGNGVLDQGEDRDGDRRLDDGTEDRNGNEMLDDNSFPTDLYPYGRLTPR
                     PADRDYSIDLRNGVIDGPHYLDYDDQRERGTLRQDLSLFLARSQGTHDVKIGYLLEKE
                     QFARASEAHAVTAVNDPGWKTGNVIDQVRNPGVQYDCNPYYDPCVDPGVGRLTAILPT
                     TIDADQSADSLSGGVYVQDAWKPIPTVSLSLGVRFDREAMTTSGYTFFEPDVERGRYD
                     RLLALAGGETGLDDLTAGNNDGVESRGIPADPLLLAAGTTGTVAEPILNGLRRAAYAS
                     LTLHRDSAQFSLAQLNAFYPGLVEDGQIDQALAASYGIQLQQPETFRITNNNLAPRIG
                     ASWDPQGDGRTKIFGAWGRYYDRLFLNTIVGEQGIERTQRYYVLDRDAIDLDQSRIQG
                     VPVVFGSVPNHQYGGRISASPPTVTQVDRDLKTPYSDEWILGFEREIAAETALSVRAV
                     QRRYKDQLQDVDVNHETRIDGFTGELSDRFGIVTEVESTDDPDIPPVVTRASDGRPDL
                     FVRNHFFNQVLRVGNSNSARYSAIEVELRRRMARRWQMQGSYTYSRAIGAAEDFQSRL
                     GNDPSVVESEFGYLDFDQRHVVKLNGGFFLPGDWQIGVASQWASGLPYSIVSRFFALD
                     NVDYEQFRTRYGFTEFDGEQLKFRSISRNSERNGSTLNIDASARKNFVWGRTTWGVSL
                     EVFNLLNRDELRIYTYEPSR"
BASE COUNT          418 a          942 c          946 g          515 t
ORIGIN      
        1 cgcggctcgg ctcgtaggtg tagatccgca gctcatcgcg gttgaggaga ttgaagacct
       61 cgagcgacac gccccaggtg gtcctccccc agacgaagtt cttgcgggcg ctggcgtcga
      121 tgttcagcgt cgagccgttc cgctcgctgt tgcgcgaaat ggatcggaac ttcagctgct
      181 ccccgtcgaa ctccgtgaag ccatacctgg tccggaactg ctcgtagtcg acgttgtcga
      241 gggcgaagaa gcgcgacacg atcgagtacg gcagcccgct cgcccactgg ctcgccacgc
      301 cgatctgcca gtcccccggc aggaagaacc cgccgttcag cttcaccacg tggcgctggt
      361 cgaaatcgag atagccgaac tcgctctcga cgacggaggg atcgttgccg aggcgcgatt
      421 gaaagtcctc cgccgcgccg atggcccgcg aataggtgta cgagccctgc atctgccatc
      481 ggcgcgccat gcgccgccgc agctccactt cgatcgcgct atagcgggcg gagttgctgt
      541 tccccacccg caacacctgg ttgaagaaat ggttgcggac gaacaggtcg ggccggccgt
      601 ccgacgcgcg ggtgacgacg ggcgggatgt ccggatcatc cgtggattcg acctcggtga
      661 cgattccgaa ccgatcgctc agctcgcccg tgaagccatc gatgcgggtt tcgtggttga
      721 cgtccacgtc ctggagctga tccttgtagc ggcgctgcac cgcccggacc gagagggcgg
      781 tctcggcggc gatctcccgc tcgaagccga ggatccactc gtcggaatag ggggtcttga
      841 gatcccggtc cacctgggtc acggtcggcg gcgaggccga aatgcgccca ccgtactggt
      901 ggttgggcac gctgccgaag accaccggca ccccctggat ccgcgactgg tccagatcga
      961 tcgcatcgcg atcgaggacg taatacctct gggtcctctc gatcccctgc tcgccgacga
     1021 tggtgttgag gaacagccgg tcgtaatagc ggccccaggc gccgaagatc ttggtgcgtc
     1081 cgtccccttg cggatcccac gatgccccga tgcgcggcgc gaggttgttg ttggtgatcc
     1141 ggaacgtctc cggctgctgc agctggatcc cgtaggacgc cgccagcgcc tgatcgatct
     1201 gtccatcctc caccagcccc ggatagaacg cattgagctg ggccaggctg aactgcgcgc
     1261 tgtcgcgatg gagcgtcagg ctcgcgtagg cggcgcgcct gaggccgttc aggatcggct
     1321 cggcgaccgt tcccgtcgtc cccgcggcga gcagaagcgg gtcggccggg attccgcggc
     1381 tctcgacccc gtcattgttc ccggcggtga ggtcgtccag cccggtctct ccccccgcca
     1441 gcgccagcag gcggtcgtaa cggccccgct ccacatccgg ctcgaagaaa gtgtagccgc
     1501 tcgtggtcat cgcctcgcgg tcgaagcgca cgccgagcga caggctcacg gtggggatcg
     1561 gcttccaggc gtcctggacg tatacgccgc cgctgagcga gtcggccgac tggtcggcgt
     1621 cgatggtggt cggcaggatg gcggtcagcc ggccgacgcc gggatcgacg caggggtcgt
     1681 agtagggatt gcagtcgtac tggacgcccg gattacggac ctggtcgatg acgttgccgg
     1741 tcttccagcc cggatcgttg acggcggtca ccgcgtgtgc ttcggacgcg cgggcgaact
     1801 gctccttctc cagcaggtac ccgatcttga cgtcgtgcgt cccctgggac cgcgcgagga
     1861 agaggctgag gtcctggcgc agggtgcccc gctcccgctg gtcgtcgtag tccaggtagt
     1921 ggggtccatc gatgacgccg ttgcgcaggt cgatggaata gtcgcgatcg gccggccggg
     1981 gcgtcagccg accgtaagga tagaggtcgg tcggaaagga attgtcgtcc agcatctcgt
     2041 taccgttgcg gtcctccgtc ccgtcgtcca ggcgccggtc gccgtcacga tcctcgccct
     2101 ggtcgagcac gccgttgcca ttgcggtcct cgttccgagt gtcgaggcgg ccgtcgcagt
     2161 cccggtcctc gcgcccatcc ccctcgcatc cccccggcgg cgtgagacgg cgatccttgt
     2221 cccctttcga gtgggtcagc cagttgtcgc ggatctggta accgtcctcg tcgtccagga
     2281 acgtggtggg gaagggacac gaatagggcg gggtcggcct ccacagaccg tcgacgaagc
     2341 agtagggcgc ctccccgatg ctcaggacgc cgtcccgggc gatggtgtcc tcgaagatgt
     2401 cgaagcggcc gtcgccgtcg tagtcttctc cggcgtcgcg ctcgcgcgcg tcgcggaagc
     2461 cgtcgccgtt gaaatccgtc gacacgaggc cgtcgccgtt gttgtcgagc ggcaggttcg
     2521 gcaccaccgc cgggcggccg tcgaggtacg acacggtcgt ctcgagggcc gcggacgggc
     2581 cgaggaccgt cgtgccccgg gcgctgtaga gcggccctcc ggccttcagc gtgtaccccg
     2641 cttcctgacg cgtcaggctg ttcagtccct ggttggtgaa ctcctgcggg tcgtagttca
     2701 cgctgaacac cagccgcgtg ttctgcgtcg cctgccacgt ccctttcgcg aacagacgct
     2761 tttcctcgag cccggtcacg aatgcctgcg tcaccgcgtt caccgggtct tccctctgga
     2821 t
//
LOCUS       DASRIQ010000470         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_722553, whole genome shotgun sequence.
ACCESSION   DASRIQ010000470 DASRIQ010000000
VERSION     DASRIQ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722553"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3931
                     /locus_tag="VFD06_11455"
     CDS             <1..>3931
                     /locus_tag="VFD06_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZN04187.1"
                     /translation="RFPAAIAALPPARCAEPFVQLDPASGSVGPGESRAITVTFDATS
                     LAGGTYAADVLLATNDPSAAVVPLRAGLEVSGSPAMEVALEDVSVSSESLYFTSAAAT
                     VHDFSLPDPAPVAGDIELEATGNFGSIMQSARAMTSQGSVVEVGGTGLDCAPASLRRT
                     LPDAALNVPSGQTLRVQVQNSPTVGAYCGVNRHRLTLRFYRRADRIDFGTQVSQSEAT
                     RELLVLNRGHATLTASAISDNPAATVSPASFQVPAGGRRFLTVRVSVPAAAISGRIEI
                     TGDAPATPQWSIPWSAEGRPAPALSVTPDLLEASLKTGEERTLPLTVANPGGMDLVYS
                     IAAEPAGSDWLVAPAGGVLPAGGEAQHGVTLRAGMRPPATYSATLRITSNDPSRPTLQ
                     VPVRMVIEPSPRIVPESVIASLSSLRAFYREGEETQHAFPVTAATLQAAQILVNLQGD
                     FDSFGEQAVVLLEGGIPLATLRGSIHCGSHDFSVSASAGVLRQAASDGIVRLSVVNST
                     SVQPSCPASRHRVDLLVFSSPTEVDFGSVHPGGIVRRDLRLRNAGFGSLVVSGLTGDV
                     PFTATPPSLSIAPSLSGLVTLEYHPGATGDHTATLVLQSNDPVNPVISVPLRGRAIAT
                     PAARLEPEVIDVFVPMGGQSIAAATLSNDGPGSLAYRIGQRLTAPPPPGGPFPDRFDE
                     LPMSPFPLSGVVADEATGTVYAVQYAGNQFVSLQTGAATWDPLTPLPLPTTEGAGAAW
                     LGGRLYVSFVGRNALGVYKTDLEPMEGSWSLVEWPGLLGSAIASDGVRFLYLANDSIF
                     ARFEPASGSIDLLPPFPGRMDRVGGMAYHDGVIYAHRGSLPHTFARFDLARGAWELLP
                     PLPAGALMGAAFDHARGEYLAVGPNGGSNLYRFSIQSGTWRWTSLPEHTVTFGGIAWA
                     PGPLPGAVVVQGRGGVGVTRVDNSPPLLFAGPGSGTLAPGASISLPLTVNAGSRLPGR
                     YQQQVVIESDDPVRPSIPLRVNATITADIDLDGVLDPDDNCPRHPNPDQEDIDSDGHG
                     DVCDVCPHETDPGQADADEDGIGDACDACNDPDHDGFGTAGAVPSTCPIDNCPAHANA
                     DQADSDADGTGDPCDPCDDPDRDGTADRDSPVRVCTLDNCPGLPNPGQADRDGDHLGD
                     VCDPCPDDGNPDVDGDGACSDNCPAIANSAQEDADGDGHGDRCDVCPQHADPGQADVD
                     TDGLGDICDGCSDPDGDGTGTTGAVPPTCPIDNCPLFPNADQADADADGTGDACDPCH
                     DPDRDGFADLDLPGRACAFDNCPGVPNPTQADRDGDRLGDACDPCPDD"
BASE COUNT          560 a         1439 c         1323 g          609 t
ORIGIN      
        1 gcgctttccg gccgccatcg cggcgctgcc gccggcccga tgcgccgaac cgttcgtcca
       61 gctggatccg gcctccggca gcgtcggccc gggggaatcg cgggcgatca cggtgacgtt
      121 cgacgccacc tccctcgcgg gcggaaccta cgcggccgat gtcctgctgg cgacgaacga
      181 cccgtcggct gcggtcgtgc cgctgcgcgc cggtctggag gtgtcgggaa gccccgcgat
      241 ggaggtcgcg ctggaagacg tctccgtgtc ttccgaatcg ctctatttca cctcggcggc
      301 ggcgaccgtg cacgatttct ccctgccgga tcctgcgccc gtcgcgggtg acatcgagct
      361 tgaggccacc ggaaatttcg gaagcatcat gcagtccgcg cgcgccatga catcccaggg
      421 gagcgtcgtg gaggtcggag gcacggggct cgactgcgcc ccggcgagcc tccgccgtac
      481 cctgccggat gccgcattga atgtcccctc cggccagacg ctccgggtgc aggtgcagaa
      541 ctcaccgacc gtcggcgctt actgcggggt caaccgtcac cggttgacgc tgcgcttcta
      601 ccggcgcgcc gaccggatcg atttcggcac gcaggtctct caatcggagg cgacccgcga
      661 gctcctcgtt ctcaaccggg gccatgcgac cctcacggcc tcggcgatct ccgacaatcc
      721 ggcggcgacg gtgagtccgg cgtcgttcca ggtgcccgcc ggtggaaggc gcttcctgac
      781 cgtgcgggtc tccgtgccgg cggcggcgat ctccgggcga atcgagatca ccggcgatgc
      841 tcccgcgaca ccgcaatggt cgatcccgtg gagcgccgaa gggcgccccg cgccggccct
      901 ctccgtgacg ccggatctgc tcgaagcgtc cctgaagacg ggagaggaac gcacccttcc
      961 gttgaccgtc gcgaacccgg gggggatgga cctggtctat tccatcgcgg cggagcctgc
     1021 cggaagcgac tggcttgttg cgccggccgg cggtgtcctg ccggccggcg gcgaggcgca
     1081 gcatggcgtc acgctgcgtg cggggatgcg cccgccggcg acctattccg cgacgcttcg
     1141 gatcacgtcg aacgacccct cacgtccgac gctccaggtc cccgtgcgga tggtgattga
     1201 gccctccccg cgcatcgtcc cggaaagcgt gatcgcgagt ctgtcgtcgt tgcgcgcctt
     1261 ctacagggag ggagaggaaa cccagcacgc cttccccgtg acggcggcga cactgcaggc
     1321 ggcacagatc ctggtcaacc ttcagggaga cttcgactcc ttcggcgaac aggccgtggt
     1381 cctcctcgaa ggggggatcc ctctggccac cctccgcggc agcattcact gcgggtcgca
     1441 cgacttctcc gtgtccgcga gcgccggcgt gctgcgccag gcggcgtcgg acggcatcgt
     1501 ccgcttgtcg gtggtcaact cgacgtcggt ccagccatcc tgtccggcga gccggcatcg
     1561 tgtggatctc ctggtcttct cgtcaccgac ggaggtcgat ttcgggtcgg tccatcccgg
     1621 ggggatcgtg cggcgcgacc tgaggctccg caacgctggc ttcgggagcc tggtcgtctc
     1681 cggcctcacg ggagacgtcc ccttcaccgc gacacctccg tccctgagca tcgcgccctc
     1741 gttgtcgggg ctggtgacgc tcgagtacca tccgggcgcg accggcgacc acaccgcgac
     1801 gctcgtcctg cagagtaacg acccggtcaa tcctgtgatc agcgtgccgc tgcgcggccg
     1861 cgcgatcgcc acaccggcgg cgcgactcga gccggaggtg atcgacgtct tcgtgccgat
     1921 gggcggacag tcgatcgccg cggcgaccct ctcgaacgac gggccgggga gcctcgccta
     1981 ccggatcgga cagcggttga ccgccccgcc tccccccggc ggcccgttcc ccgaccggtt
     2041 cgacgagctt ccgatgagtc ccttcccgct cagcggcgtg gtggccgacg aggcgacggg
     2101 gacggtgtac gcggtccagt acgccgggaa tcagttcgtc tccctgcaga ccggtgcggc
     2161 aacgtgggac ccactcacgc cgctccccct cccgaccacg gaaggcgccg gcgccgcctg
     2221 gctcggcggg aggctgtacg tctcgttcgt cggccgtaat gcgctcgggg tctacaagac
     2281 ggacctggaa ccgatggagg gatcctggag cctcgtcgag tggcccggcc ttctcggcag
     2341 cgccatcgcc agcgatggtg tccgcttcct ctacctggcg aacgattcga tcttcgcccg
     2401 cttcgagccg gcgagcgggt cgatcgacct cctgcctccg ttccccggcc ggatggaccg
     2461 cgtcgggggg atggcctacc acgatggcgt catctatgca catcgcggtt cgctccccca
     2521 cacgttcgcc cgcttcgacc tcgcccgggg tgcctgggag ctcctcccgc cccttcccgc
     2581 cggcgcactc atgggggccg ccttcgatca cgcgcggggc gagtacctgg ccgtcggccc
     2641 gaatgggggc agcaacctct atcgcttctc catccagagc ggcacctggc gctggaccag
     2701 cctgccggag cacacggtca ccttcggcgg gatcgcctgg gcgccgggac ctctgcccgg
     2761 ggcggtcgtc gtgcagggac gcggcggcgt cggggtgacc cgcgtggaca atagtccgcc
     2821 gcttctcttc gccgggccgg gctccgggac tctggccccc ggcgcatcga tctccctgcc
     2881 cctgacggtc aacgcaggtt cgcgtcttcc cggccggtat cagcagcagg tcgtcatcga
     2941 aagcgacgac ccggtccggc cctcgattcc gctgcgggtg aacgccacga tcacggcgga
     3001 tatcgacctc gacggcgtcc tcgatcccga tgacaactgt ccgcgtcacc cgaacccgga
     3061 ccaggaggac atcgactccg acgggcacgg ggatgtctgc gatgtctgtc ctcatgagac
     3121 cgacccgggg caggccgacg ccgacgagga cgggatcgga gacgcctgcg acgcctgcaa
     3181 cgaccccgat cacgacggat tcggaaccgc cggtgcggtt ccgtccacgt gcccgatcga
     3241 caactgtccg gcgcatgcca acgccgacca ggccgactcc gacgcggacg gcacgggcga
     3301 tccctgcgac ccgtgcgacg accccgatcg ggacggcacc gcggaccggg acagtcccgt
     3361 ccgcgtctgc acgctcgaca actgtcccgg cctgccgaat ccggggcagg cggatcggga
     3421 cggcgaccac ctcggcgatg tctgcgaccc gtgtcccgac gacggcaatc cggatgtgga
     3481 cggggacggc gcctgctccg acaactgtcc tgcgatcgcg aactccgcgc aggaggacgc
     3541 cgacggtgac ggacacggcg accgctgtga tgtgtgcccg cagcatgccg atcccggcca
     3601 ggcggatgtc gatacggacg gtctgggaga catctgcgac ggctgcagcg accccgacgg
     3661 cgacggaacc gggacaacag gggccgtgcc ccctacctgc ccgatcgaca actgcccttt
     3721 gttccccaac gcggaccagg ccgacgccga tgcggacggc acgggcgacg cctgcgaccc
     3781 gtgccacgac cccgaccggg acggtttcgc ggacctcgac cttcccgggc gcgcctgcgc
     3841 gttcgacaat tgccccggcg tgccgaatcc gacgcaggcc gatcgggacg gcgacaggct
     3901 cggagacgcc tgcgacccgt gtcccgacga c
//
LOCUS       DASRIQ010000471        13503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2516944, whole genome shotgun sequence.
ACCESSION   DASRIQ010000471 DASRIQ010000000
VERSION     DASRIQ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 13503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13503
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516944"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFD06_11460"
     CDS             <1..647
                     /locus_tag="VFD06_11460"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZN04188.1"
                     /translation="RLTRGAETRTLAWQGGEIVFARSNSVRNTLGNFLVRKGIITPEQ
                     NALSAAKIDESTRHGKVLVRMGFLSADQLHWAVKQQVLEIIYSLFHWRTGLFEFVEGP
                     IESQEKITLSMSTTRIIMDGIHRLDEWTKMRDAIPDDNGVPEPLARNDEVAKRADLGG
                     DEKKVFPLVDGARTVADIVEQARLGEFESYAALFQLITAGVVRMAGRAAAATGT"
     gene            complement(661..2160)
                     /gene="rlmD"
                     /locus_tag="VFD06_11465"
     CDS             complement(661..2160)
                     /gene="rlmD"
                     /locus_tag="VFD06_11465"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZN04189.1"
                     /translation="MEARRVDDRPLAQDRRRLRRTAAAPRSRGRARHRPVGPLNPNPP
                     APGAAIEIRIDDLARAGRGVGRHEGLVVFVPGALPGETARVRITRLRRGYAEAEIVAI
                     VAPSSERRRPPCPFFESCGGCDLQHLSEAGQAEAKRRQVAEALRRIGTLGDVEVAPTR
                     RIGAPLGYRFRMDFDWRRQRGRAVLGLHRRGSSSEIVPVDPCLLAGPVTAAIAAWIPA
                     AAARAGLTALDERSGRGLLRRLTVQEARGTREVLVTLETGRGDPPALAGLAADLVRRV
                     PRVVGVVRHESDRLGHFVGTSILAGRNHLEEELEGDRFRVPAGAFFQPNPEGSLAVRQ
                     AALESLAVVPGMRLLELYAGVGFFSVEAARRGAVLTLVESDRDATEAARENLRRSGAA
                     GFLVLRNDVTRSLIDLAREPWDGILLDPPRTGLAPEGSKLLANIAAPRMAYVSCDPGT
                     LARDLGILVRTGGWEIEKVTPFDLFPQTQHVECVAVLHRPRPEAGSSES"
     gene            complement(2043..2489)
                     /locus_tag="VFD06_11470"
     CDS             complement(2043..2489)
                     /locus_tag="VFD06_11470"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HZN04190.1"
                     /translation="MAEEPPYRRLFTLDEARALVPLIQPAIEDLVGTFRVIREAIEET
                     AGQSGHAIGSRDLAGLLEQQGTVPRLFERVKAILGTIQSHGCLVNGAEVGLVDFPCMY
                     NNEVVFLCWKHGESTIGHWHRIADGFAGRRPLLDPGDGPGTAPSVH"
     gene            2603..4378
                     /locus_tag="VFD06_11475"
     CDS             2603..4378
                     /locus_tag="VFD06_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN04191.1"
                     /translation="MRSIRSLLPPLLLVAAFALSAGRVEATCGNSLSFRHALDSYWTG
                     LPEVNLISMAYSLENPSVATGEAPVLCRFFGEETSGGQCQPQAGSASDGVVTVNGNWD
                     HPKAGGCPNQAGEWGHPIVIAATAAVDEGSPGHRGVGIVASVGYDVDSGGYLLDWAQP
                     FAEGGNDVRPVAAQDIPVPQVTGVRSSADGFLLVDLNWNAFQTFDDCTQTFRPTCTDS
                     KGRRRPVLTQFMVYSARSSCSSPPLSGLLTSGLWSPVGTVSSNVSVATRVPNPGGECA
                     WFAVGLVLQGGYLPPVVSGNSRGVNLATAGGEDKKGDKPGEKKPGDSDAPGDGDQGAD
                     PGDAGKTGSDSGGSTPGESAGGAAGQQAADAAAPGAAQEKKEPCVDEDDIPDDEDNCP
                     CDTNPKQEDVDFDGVGNRCDRCPVVPDPEQKDADRDGLGNPCDNCPDAANAKQEDRDA
                     DGVGDACDNCPDHSNPPQDDADKDGRGDACTQAIVEARRIRGPDGSRVEWRITHEFDL
                     VGIDLFAVDPKGKERRLRDKPIPCRACKTGAPETYKVPLTSEEDKGTLVLRLVRVGGA
                     ADERPVRLDVPAPAPTPSAAPAKPR"
     gene            complement(4388..4966)
                     /locus_tag="VFD06_11480"
     CDS             complement(4388..4966)
                     /locus_tag="VFD06_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZN04192.1"
                     /translation="MQRAWRRASGLVIIALTASLGMTPAAAADLSKQWEVGADLLSTT
                     MDNDTTIDDALGWRARGAYHFNKTHAVELTYDDVSADSNIKGSVLEYDLQKIMVTYLG
                     DFKNKKPDAKWQLFALFGAGRIDYDNGFDSEASTVLQTGGGVRIMLSKSWALRLDGRI
                     WHFHGDSRIIPRDGFFSFDLALGASYLFGKGK"
     gene            5106..6797
                     /locus_tag="VFD06_11485"
     CDS             5106..6797
                     /locus_tag="VFD06_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZN04193.1"
                     /translation="MITGRAIRLLLAAGPPFAAAAAIGFVIPIAVIAADPDPRIAAIG
                     SIRFVGSGAEGAEGTVFTSTRLRAFLEEEVGRPCAPLRIEEGIASRYRALGYVPTVRA
                     WCADGELVLAVRESSHRVAVVTFDVPDLAPLGVQPQRIADEKPLYPVPESAPRAVVRG
                     LLQTRPGDLYNVERYRLDRLALARLGYALLFIPGAAPSEEQIAGGAYLIQSTTQMPED
                     ETRRLRRGPLNYLGGSAGYGPRTGGTAGVVYQRRDILTGLDTLSLTPTFSTAWGGEIG
                     YSAPVVAAHKAPKRIYDVGGSLFTAFVNNRELEGDERDERRTGGSLNLGARPLGLPSP
                     HDLHLQAELRRETVDVDGLDESDLSILRLSAVHEYRHIYRRPSLLLRTVPALEGSFDV
                     GDGIPYVRPALQVTAHGRFRTGIEYELRLGGGGIDRPVPQFQLFTLGGATTVRGFQED
                     TFLGRGIAYLQAELWIPFIRPLEGRKVGQEEIADPSRAPFEPRLARAIKGAVFVDGGG
                     VWQSPLIGRASIAGAGVGIRFVVPGEPLVVRIDYAWGFGEHGGDSYPYIALGYGF"
     gene            6812..7201
                     /locus_tag="VFD06_11490"
     CDS             6812..7201
                     /locus_tag="VFD06_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZN04194.1"
                     /translation="MTVTDDGTDRDGRQLALQELLTTLRRDADARYRHHLAAQISGES
                     AGDSEGVHGWKASREGGADVALLESLDQTVRQIDAALERLNTGAYGRCDACGEGIPIA
                     RLRAVPFATRCVPCQSAKEDGGSGRRA"
     gene            7255..8562
                     /locus_tag="VFD06_11495"
     CDS             7255..8562
                     /locus_tag="VFD06_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN04195.1"
                     /translation="MPRRAGLLAACGLLYASACGGPYGTEDFVAQVGPGLLTLLIVVG
                     AVIWGLPLSLATAELSARRPIEGGYYRWVREFLGEDWGCRAGLWSLMSSVLDNALYPV
                     LFAQALEYVLPGLGAFPRWLAAVGFIALLTWLNIRGIRIVGAAAVALNAFLIAPLVWM
                     VVAAVPRWQFNPFLPFSAGESPLDGLSAGLALAVWFHSGYSEVSTAAEEIEQPRRTIP
                     LVLLIVTPLVIVSYVLPMIVGLAATGGWQNWESGHFATIGRMLGGPVLGHWSLLASVA
                     SFTVIFMAYLLWWSRLAWAFAADGFLPRWLVERHPSYGTPHRVLILYAILYALLALVP
                     FKELLIVDVWLFGAYDALVVWSLLRARRSAVVDPEGFRLPGGTAGVVINAAVLTLTWG
                     VLLVTTAREDPYAALAGAALLAAGAIATPFLGRLKTRGRPAGS"
     gene            complement(8531..10693)
                     /locus_tag="VFD06_11500"
     CDS             complement(8531..10693)
                     /locus_tag="VFD06_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04196.1"
                     /translation="MTRVVTKGAVAAFLLVASMSAEFRTGAADPPRPRAKGKASPSAA
                     GPRLPSAIPEFERAADSAIAAIVSKHGAGSKVRAERGVKQVLRAWRQEDGAPSEFERL
                     VTTEFFPEGAELDGCFDRLEALLERIDGYFLSLGRDLRFTLEVETGPIRPIDERLGAW
                     DPGAHVQDDLFGNGTAFVVLVNFRLTTLDERLKEGPGWTRRQWAEARLADRFARRLPA
                     EVSQQIQRAYVEAEAYIARDNIMMHHLLDEKGGRPFPAGMRLLSHWNLRDEIKALYSD
                     PAGLASQRMIARVMERIVTQEIPEAVIDNPLLDWNPVTNAVTRSSVRDLEPPPGKSAT
                     PSNAREPDTRYRTWMAIAAAARRADPFDPEHPNLIARRFEVDREIPEAETKALLLAVL
                     EAPIAQDAARLIAQRLGRPLEPFDIWYAGFKPRSRYTEADLDARTKARYPTAADYAAD
                     MPRMFKELGFDREQARFLAANIEVDPSRGSGHAQGAERRDDQVRLRTRVGPGGMDYKG
                     YNIAVHEMGHNVEQSFSLDRIDHWFLHGVPNNAFTEALAMVFQGHDVELLGLEKPSGD
                     AEASRALEEFWGTREIAGVALVDMAAWHWLVAHPDGTPAQFREAVVGAARDVWNRHFA
                     DLVGARDVPLLAIYSHMVSSALYTPDYPLGHLIAFQLEEHFRKQKKMGPEFERVCRIG
                     RVTPDLWMRQAVGGPLSAAPLLDAAARALRNPPAGPAS"
     gene            complement(10690..11517)
                     /locus_tag="VFD06_11505"
     CDS             complement(10690..11517)
                     /locus_tag="VFD06_11505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN04197.1"
                     /translation="MTTAGGGRVRTALVTGASSGIGLELCRLLARDVARVVMVARDEG
                     RLQRAARWVQAEVKTAALTLVPADLSGPGAATALHARVERDVGPVDFLANNAGAGLSG
                     RFAETDAEATAALVALNVTSLTELTRPYLRDMLARRSGRILNVASTAAYLPGPGMAVY
                     YATKAFVLSFSEALAEETAGSGVTVTALCPGPTATGFWERAGIAGTRLLRSPLVMPVA
                     EVAAAGYRGALEGRRVVIPGLANRLMVEGLRFLPRSVAAAVVGRAHQPAGGDEGGDA"
     gene            11742..>13503
                     /locus_tag="VFD06_11510"
     CDS             11742..>13503
                     /locus_tag="VFD06_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZN04198.1"
                     /translation="MRRDPRILAALVLVLLVSVAGGMAATPPARGLHPGLDATHGGGL
                     LPDGSQPRAMAAADFDEDGMADVAVAFSDGRRGVIALLIGNRDVVDASEPEARRRRAK
                     NGLEPRALPPFRVEGPLVDLPAPADLAGAGDFDNDGHLDLVVAAEGGRTLLLLRGDGR
                     GGFADPLPRSLPGRLSRMVVGEMNHPDSLADIVVAVERQHQTEILVYEDPEGAWKAEP
                     ESVRRGGPITMLALGDLNGDFERDLLVGIDRGIEILHGRDRKLALGLGWRATVAAPAL
                     ERHPLDCTPRRAAVGRFGGTTGPNLAFLADDGTVRLAAAGTLASSRIVARGRGGRLLV
                     GARLGDGVGDDLVTIQEDGSRLRALAGLDVPLEFEPLDALSMRLGPEAVESLVVLNGS
                     GPDLLSILPPAAPTTYTVTTIANSGPGSLRDAILSANTNAGADTIDFNISPSANLTIV
                     PATPLPEVTDPVTIDATTQPGYPGTPIVEIDGSGPLPPGTNGLVVRAGQSVVRGLVIH
                     GFSGSGVLLDGAGHNIVEGNFIGVDAAGTTAIGNQDSGVVIRDSHLNTIGGTAAPARN
                     VLSGNAQAGVAIRGPSTFEAE"
BASE COUNT         1896 a         4889 c         4749 g         1969 t
ORIGIN      
        1 tgcgcctgac ccgcggggcg gagacccgca ccctcgcctg gcaggggggg gaaatcgtct
       61 tcgcacgctc caactcggtg cgcaacaccc tcgggaactt cctcgtccgc aaagggatca
      121 tcacgccgga gcagaacgcg ctgtcggcgg cgaagatcga cgagagcacc cgtcacggca
      181 aggtcctcgt ccggatgggg ttcctgtcgg ccgaccagct gcactgggcg gtcaagcagc
      241 aggtccttga aatcatctat tccctcttcc actggcggac cggcctgttc gagttcgtcg
      301 aggggccgat cgagagccag gagaagatca ccctgtcgat gtccacgacg cgcatcatca
      361 tggacggcat ccatcgcctg gacgagtgga cgaagatgcg cgacgcgatc cccgacgaca
      421 acggcgtgcc ggaacctctc gcgcgcaacg acgaagtggc gaagcgggcc gacctcggag
      481 gcgacgagaa gaaggtcttc ccgctcgtgg acggggcgcg gacggtggcg gacatcgtgg
      541 agcaggcgcg gctgggagag ttcgagtcct atgccgccct cttccagctg atcaccgcgg
      601 gcgtcgtgcg catggccgga cgtgccgcag ccgccaccgg aacctgaccc ttccgttccg
      661 tcacgattcc gatgacccag cctccgggcg cggccggtgg agcacggcca cgcactcgac
      721 gtgctgggtt tgcgggaaga gatcgaacgg ggtgaccttt tcgatctccc agcccccagt
      781 gcgaacgagg atgccgagat cgcgggcgag ggtgccgggg tcgcaggaga cgtaggccat
      841 cctgggcgcg gcgatgttcg ccaggagctt gctgccttcc ggggcgaggc cggtgcgggg
      901 agggtcgagc aggattccgt cccacggctc acgcgccaga tcgatgaggg agcgggtcac
      961 gtcgttgcgc aggacaagga agccggcggc gccgctgcgc cggagattct cgcgggcggc
     1021 ttcggtcgcg tcccggtccg actcgacgag ggtcaggacg gcgcctcgac gcgccgcctc
     1081 gacgctgaag aagccgaccc ccgcatagag ctccagcagg cgcatcccgg ggacgaccgc
     1141 gagtgattcg agggcggcct gccgcaccgc gaggctcccc tccgggttcg gctggaagaa
     1201 cgccccggcc ggaacgcgga accgatcccc ctccagctcc tcctcgaggt ggttccgtcc
     1261 ggcgaggatc gacgtgccga cgaagtgccc cagacgatcg ctttcgtgac ggaccacgcc
     1321 gacgacgcgc ggcacgcggc gcaccagatc agccgccaga ccggcgagcg ccggcgggtc
     1381 gccccgcccc gtctcgaggg tcacgagaac ctcgcgcgtg ccgcgcgctt cctggacggt
     1441 gaggcggcgc aggagccctc gcccgctccg ctcatccagc gccgtgagcc cggcgcgcgc
     1501 ggcggcggcc gggatccatg ccgcgatcgc cgcggtgacc ggcccggcga gaaggcaggg
     1561 atcgaccggc acgatctcgc tcgacgaccc gcgccggtgc agtcccagga cggcccgccc
     1621 ccgctgccgc cgccagtcga agtccatccg gaagcgatag ccgagcggcg cgccgatccg
     1681 ccgcgtcggc gcgacctcga cgtcacccag cgtcccgatg cggcgcagcg cttcggccac
     1741 ctggcggcgc ttcgcctccg cctgccccgc ctccgacagg tgctgcagat cacagccgcc
     1801 acacgactcg aagaaagggc acgggggccg gcggcgctcg gacgatgggg cgacgatcgc
     1861 gacgatctcc gcttccgcat agccgcggcg caggcgcgtg atgcggacgc gggccgtctc
     1921 ccccggcagc gcgccaggga cgaacacgac gagcccctcg tgccggccga cgccgcggcc
     1981 ggcgcgcgcc aggtcgtcga tacgaatctc gatcgcggcg ccgggcgccg gagggttggg
     2041 gttcagtgga ccgacggggc ggtgccgggc ccgtccccgg gatcgaggag cggccgccgt
     2101 ccggcgaagc cgtcggcgat cctgtgccag tggccgatcg tcgactcgcc gtgcttccag
     2161 cagagaaaca ccacttcgtt gttgtacatg caggggaaat cgacgagccc gacttcggcg
     2221 ccgttgacga ggcagccgtg gctctggatg gtcccgagga tcgccttcac ccgctcgaac
     2281 aggcgcggca ccgtcccctg ctgctccagg agcccggcca ggtcgcggct cccgatggcg
     2341 tgcccgctct gtccggcggt ctcctcgatc gcctcgcgga tgacccggaa ggtgcccacg
     2401 aggtcctcga tcgcgggctg gatgagcggc acgagggcgc gtgcttcgtc gagggtgaac
     2461 agtcgccggt aagggggctc ttcggccatg atggaggctc cgcggggatt ctagatcggg
     2521 ggacgacgcc ttgacagggc ctcttttgat cgtacataca gggccgaccg cgccccgccg
     2581 ccgccgggac gcgccgatgc cgatgcggtc catacgctcc cttcttccgc ccctccttct
     2641 ggtcgcggcg ttcgcccttt ccgccgggcg cgtcgaggcg acctgcggca attcgctatc
     2701 gttccgccac gcgctggact cgtactggac cgggctcccc gaggtgaacc tcatttccat
     2761 ggcctattcc ctcgagaatc ccagcgtcgc gaccggggag gcgccagtcc tgtgccgctt
     2821 cttcggcgag gagacctcgg gaggacagtg ccagccgcag gcgggatccg cctcggacgg
     2881 cgtggtcacc gtgaacggga actgggacca tccgaaagcc ggcggctgcc ccaaccaggc
     2941 tggcgaatgg gggcacccga tcgtcatcgc cgccaccgcg gccgtggacg aaggatcgcc
     3001 cggccatcgc ggcgtcggga tcgtcgcgtc ggtcggatac gacgtggact cgggcggtta
     3061 tctcctcgac tgggcgcagc ccttcgcaga ggggggcaac gacgtccggc cggtcgcggc
     3121 gcaggacatc ccggtccccc aggtgaccgg ggtccggtcc tccgcggacg ggttcctgct
     3181 ggtcgacctg aattggaatg cgttccagac gttcgacgac tgcactcaga ccttccgccc
     3241 gacctgcacc gactccaagg gccgcaggcg cccggtcctg acgcaattca tggtctacag
     3301 cgcccgttcg tcctgcagct ccccgcccct ctcgggcctc ctgacctccg ggctctggtc
     3361 gcccgtgggg acggtctcga gcaacgtctc ggtggcgacg cgcgtcccca accccggcgg
     3421 cgagtgcgcc tggttcgccg tcggcctggt gctccaggga ggctatctgc cgccggtcgt
     3481 ttccggaaac tcgcgcgggg tgaacctggc gacggccgga ggcgaggaca agaaggggga
     3541 caagccgggg gagaagaagc cgggcgattc cgacgcaccg ggcgacgggg atcagggagc
     3601 ggacccaggc gatgcgggca agaccggaag cgacagcggc ggctccaccc cgggcgaatc
     3661 ggcaggcggg gcggccggcc agcaggcggc ggacgccgcg gcgcccgggg ccgcccagga
     3721 gaaaaaggaa ccgtgcgtcg acgaggacga catcccggac gacgaggaca actgcccctg
     3781 cgacaccaat ccgaagcagg aggacgtcga tttcgacggc gtcggaaacc gctgcgatcg
     3841 ctgcccggtc gtgccggacc ctgagcagaa agacgccgat cgggacggtc tcggaaaccc
     3901 gtgcgacaac tgcccggacg ccgccaacgc gaaacaggag gatcgggacg ccgacggcgt
     3961 cggcgacgcc tgcgacaact gccccgacca ttccaacccg cctcaggacg atgcggacaa
     4021 ggacggtcgt ggggacgcct gcacccaggc gatcgtcgag gcgcgccgca tccgcggccc
     4081 cgacggcagc cgggtcgagt ggcgcatcac ccacgaattc gacctggtcg ggatcgatct
     4141 gttcgccgtg gacccgaagg ggaaggagcg ccggctgcgc gacaagccga tcccctgccg
     4201 cgcctgcaag accggcgcgc cggagaccta caaggtgccc ctcacctccg aggaggacaa
     4261 agggacgctc gtcctgaggc tggtgcgcgt gggaggagcg gcggacgaac ggccggtgcg
     4321 gctcgacgtc ccggctccgg cgccgactcc gtccgcggca cccgcgaagc cccgctgacc
     4381 gcggagccta cttccccttc ccgaacaggt acgacgcgcc gagcgcgagg tcgaacgaga
     4441 agaagccgtc gcgcgggatg atccggctgt cgccgtggaa gtgccagatg cgcccgtcga
     4501 ggcgcagcgc ccaggacttc gacagcatga tgcgcacccc gccgccggtc tggagcaccg
     4561 tgctcgcctc ggagtcgaag ccgttgtcgt aatcgatccg ccccgccccg aacagggcga
     4621 agagctgcca cttggcgtcg ggcttcttgt tcttgaagtc cccgaggtac gtcaccatga
     4681 tcttctggag gtcgtactcg aggacgctcc ctttgatgtt ggagtcggcg gagacgtcgt
     4741 cgtaggtcag ctcgacggcg tgcgtcttgt tgaagtggta cgcgccgcgc gcccgccagc
     4801 cgagcgcgtc gtcgatggtc gtgtcgttgt ccatcgtcgt gctcaagaga tcggcgccca
     4861 cctcccactg cttcgacaga tcggccgctg cggccggggt catcccgagc gatgcggtca
     4921 gggcgatgat cacgaggccg ctggcgcggc gccacgcacg ctgcatgtcg aatcctcctc
     4981 cgttcccggc gtccccgcgt ccggggggcg ggagtatgga cttttcgggc gcggcggatc
     5041 aaccgggtgc gccgccgcgg cgcgcgactt gacaccggcc ggcgggacgg gtacgtagcg
     5101 cgccgatgat caccggccgc gccatccgtc tcctgctcgc ggcgggcccc cccttcgcgg
     5161 cggccgccgc gatcggattc gtgatcccga tcgcggtgat cgccgcggat ccggatcccc
     5221 ggatcgccgc gattggctcg attcgcttcg tcgggtccgg cgccgagggg gcggagggga
     5281 ctgtcttcac cagcacccgc ctccgcgcct tcctcgagga ggaggtgggg cggccgtgcg
     5341 ccccgctgcg gatcgaggag gggatcgcct cgcggtaccg ggccctcggc tacgtcccga
     5401 cggtgcgcgc ctggtgcgcc gacggcgagc tcgtgctcgc ggtgcgggag agcagccatc
     5461 gggtcgccgt ggtcaccttc gacgtgccgg acctggcccc gctcggggtc cagccgcagc
     5521 gcatcgccga cgaaaagccg ctctacccgg ttccggagag cgccccccgc gccgtggtgc
     5581 gcggtctgct gcagacccgc cccggcgatc tgtacaacgt cgagcgctat cgcctcgatc
     5641 gcctcgccct ggcccgcctc gggtacgccc tgctgttcat tccgggggcc gccccgtcgg
     5701 aggagcagat cgccggggga gcgtacctga tccagagcac gacccagatg ccggaggacg
     5761 agacccggcg cctccgccgg ggaccgctga actacctcgg cggcagcgcc gggtacggcc
     5821 cgcgcaccgg cggcaccgcc ggtgtcgtct accagcgccg cgacatcctc accgggctcg
     5881 acacgttgtc cctgacgccg accttcagca ccgcgtgggg gggcgagatc ggctattcgg
     5941 cgccggtcgt ggcggcgcac aaggcaccga agcggatcta cgacgtcggc ggatcgctct
     6001 tcaccgcgtt cgtgaacaac cgggagctcg agggggacga gcgggacgag cggcgcaccg
     6061 gcggttcgct gaatctcggg gcgcgcccgc tggggctgcc ttcgccgcac gacctgcacc
     6121 tccaggcgga gctgcgccgc gagacggtcg acgtggacgg gctcgacgaa tccgacctct
     6181 cgatcctgcg cctgagcgcc gtccacgagt accgtcatat ctatcggcgc ccgagcctgc
     6241 tcttgcgcac cgtgcccgcg ctcgagggct cgttcgacgt cggtgacggc atcccctacg
     6301 tgcggccggc gctgcaagtc accgcgcacg ggcgtttccg caccggaatc gagtacgagc
     6361 tgcgcctcgg cggcggcggg atcgaccggc ccgtgccgca gttccagctc ttcacgctcg
     6421 gcggcgccac gacggtgcgc ggcttccagg aggacacctt cctcggccgc ggcatcgcgt
     6481 acctccaggc cgagctgtgg atcccgttca tccggccgct cgaagggagg aaggtcggac
     6541 aggaggagat cgccgatccg tcgcgcgccc cgttcgagcc gcggctggcg cgcgcgatca
     6601 agggagcggt gttcgtggac ggcggcggcg tctggcagag cccgctgatc ggccgggcgt
     6661 cgatcgccgg agccggtgtc ggcatccgct tcgtggtccc cggggagccg ctcgtggtgc
     6721 ggatcgacta cgcctggggc ttcggcgaac acggcggcga ctcgtacccc tacatcgccc
     6781 tcgggtacgg cttctgagta tcatccgcgc cgtgaccgtg acggacgacg gaaccgaccg
     6841 ggacgggcgc cagctggcgt tgcaggagct cctgacgacc ctgcgtcggg acgccgacgc
     6901 gcgctaccgg caccatctgg cggcgcagat ctcgggggag tcggccggcg acagcgaagg
     6961 ggtgcacggc tggaaggcca gccgcgaagg gggcgcggac gtcgccctgc tcgagtcgct
     7021 cgaccagacc gtccggcaga tcgacgcggc gctcgagcgt ctgaacacag gggcctatgg
     7081 ccgctgcgac gcctgcggcg aagggatccc gatcgcgcgc ctgcgcgccg tgccgttcgc
     7141 gacccgctgc gtcccctgtc agtcggccaa ggaagacggc ggctccggcc gccgcgcatg
     7201 agccccccgg aggctcacgc ccaggttccc tctccgccgg ccggggccac ctccatgccc
     7261 cgccgcgccg gtcttctcgc cgcctgcggg cttctctacg cctccgcctg cggcggccct
     7321 tacggcaccg aggacttcgt cgcgcaggtc ggcccgggtc tgctcaccct gctgatcgtc
     7381 gtcggcgcgg tcatctgggg ccttcccctg tcgctcgcca ccgccgagct ctcggcgcgc
     7441 cggcccatcg aaggcggcta ctaccgctgg gtccgcgagt tcctcggcga ggactggggc
     7501 tgccgggccg gcctctggag cctgatgtcg tcggtcctcg acaacgcgct ctacccggtg
     7561 ctgttcgcgc aggcgctcga gtacgtgctc ccgggtctcg gcgccttccc ccgctggctc
     7621 gcggcggtcg gcttcatcgc gctgctcacg tggctcaaca tccgcggcat ccggatcgtc
     7681 ggggcggcgg cggtcgcgct caacgccttc ctcatcgcgc cgctcgtctg gatggtcgtc
     7741 gcggcggtgc cgcgctggca gttcaacccg ttcctcccgt tcagtgccgg ggagtccccg
     7801 ctcgacgggc tctcggccgg cctcgcgctc gcggtctggt tccattccgg ctactcggaa
     7861 gtgtcgaccg ccgccgagga gatcgagcag ccgcggcgca ccatcccgct cgtcctgctc
     7921 atcgtgaccc cgctcgtcat cgtgagctac gtcctgccga tgatcgtcgg actcgccgcg
     7981 accggcggct ggcagaactg ggagtcgggg catttcgcca ccatcggacg gatgctgggc
     8041 ggcccggtcc tcggccactg gtcgctcctg gccagcgtcg cctccttcac ggtcatcttc
     8101 atggcctacc tgctctggtg gtcgcgcctc gcctgggcgt tcgccgccga cggcttcctg
     8161 ccacgctggc tcgttgagcg ccacccgagt tacggcacgc cgcaccgcgt gctgatcctg
     8221 tacgcgatcc tctacgcgct gctcgccctc gtccccttca aggagctgct catcgtggac
     8281 gtgtggctgt tcggcgccta cgacgcgctg gtcgtgtggt cgttgctgcg cgcgcggcgc
     8341 tcggccgtgg tcgacccgga ggggttccgc ctccccggcg ggacggccgg ggtcgtgatc
     8401 aacgccgcgg tcctgaccct gacgtgggga gtgctgctgg tgacgacggc gcgcgaggat
     8461 ccttacgccg cgctggccgg ggccgcgctg ctcgcggccg gggcgatcgc gacgccgttt
     8521 ctcggccggc tcaagacgcg gggccggccg gcgggttcct gagggcccgc gccgcggcgt
     8581 cgagaagagg ggccgccgac agcgggccgc cgaccgcctg gcgcatccac agatcgggcg
     8641 tcacgcggcc gatgcggcag acccgctcga actccggccc catctttttc tgtttccgga
     8701 agtgctcctc cagctggaac gcgatcaggt gcccgagcgg atagtcgggc gtgtacagcg
     8761 ccgagctgac catgtgggag tagatggcga ggagggggac gtcgcgcgcc ccgacgagat
     8821 cggcgaagtg ccgattccag acgtcgcgcg ccgcgcccac gaccgcctcg cggaactggg
     8881 ccggcgtgcc gtccgggtgc gcgacgagcc agtgccaggc ggccatgtcc acgagcgcca
     8941 cgccggcgat ctcccgcgtc ccccagaact cctcgagggc ccgcgacgcc tccgcgtccc
     9001 ccgacggctt ctcgagcccg aggagctcca cgtcgtgccc ctggaacacc atcgcgagcg
     9061 cctcggtgaa cgcgttgttc gggacgccgt gcaggaacca gtgatcgatc cggtcgaggg
     9121 agaacgactg ctcgacgttg tgccccatct cgtgcaccgc gatgttgtac cccttgtaat
     9181 ccatgccgcc gggtccgacg cgggtgcgca gccggacctg gtcgtcgcgc cgctccgccc
     9241 cctgggcgtg cccggagccg cgcgacggat cgacctcaat gttcgcggcg aggaagcgcg
     9301 cctgctcccg gtcgaagccg agctccttga acatccgcgg catgtcggcg gcgtagtcgg
     9361 cggcggtcgg gtagcgcgcc ttggtgcgcg cgtcgagatc cgcctcggtg tagcggctgc
     9421 ggggcttgaa gccggcgtac cagatgtcga acggctcgag cggccggccg aggcgctgcg
     9481 cgatcaggcg cgcggcgtcc tgggcgatgg gcgcttcgag gacggcgagg aggagggcct
     9541 tcgtctccgc ctccgggatc tcccgatcca cttcgaagcg gcgggcgatg aggttcgggt
     9601 gctccgggtc gaacggatcg gcgcggcggg ccgcggcggc gatcgccatc caggtacggt
     9661 accgggtgtc cggctcgcgg gcgttcgagg gagtggcgct cttcccgggc ggcggctcga
     9721 ggtcgcgcac gctcgaacgg gtcacggcgt tggtgaccgg gttccagtcg agcagcgggt
     9781 tgtcgatcac cgcttccggg atctcctgcg tgacgatccg ctccatcacc cgggcgatca
     9841 tgcgctgcga tgcgagcccg gcgggatcgg agtagagcgc cttgatctcg tctctgagat
     9901 tccaatggct gaggaggcgc atgccggccg gaaacgggcg tcctcccttc tcgtccaaca
     9961 ggtggtgcat catgatgttg tcgcgcgcga tgtacgcctc cgcctcgacg tacgcgcgct
    10021 ggatctgctg tgacacctcc gccggcagcc gccgcgcgaa gcgatccgcg aggcgcgcct
    10081 cggcccactg cctgcgcgtc caacccggcc cctccttgag acgctcgtcg agcgtcgtca
    10141 gccggaagtt gaccagaacg acgaaggcgg tgccgttccc gaacaggtcg tcctgcacgt
    10201 gcgcgccggg atcccacgcg cccagccgct cgtcgatcgg ccggatcggc ccggtctcca
    10261 cctcgagcgt gaaacgcagg tcgcggccga gggacaggaa atagccgtcg atgcgctcga
    10321 ggagcgcttc gagccggtcg aagcagccat cgagctccgc tccttcggga aagaactccg
    10381 tggtgacgag ccgctcgaac tccgaaggag cgccgtcctc ctgccgccag gcgcgcagaa
    10441 cctgcttgac gccacgctcg gcgcgaacct tggaccctgc gccatgcttc gacacgatcg
    10501 cggcgatggc gctgtcggcg gcgcgctcga actccggaat cgcggatggc agccgcggcc
    10561 cggccgcaga cgggctcgcc ttccccttcg cccgcgggcg aggcggatcg gccgctccgg
    10621 ttcgaaactc ggccgacatc gacgccacca ggaggaacgc cgccaccgcc cctttcgtga
    10681 cgactctcgt catgcgtctc ccccttcgtc cccgcccgcc ggctggtgcg cgcggccgac
    10741 cactgccgcc gcgaccgatc gcggcaggaa tcgcagcccc tcgaccatca gacggttcgc
    10801 cagtcccggg atgacgaccc gcctcccctc cagcgcaccg cggtacccgg cggccgccac
    10861 ttcggcgacc ggcatcacca gcgggctcct gagaaggcgc gtgcccgcga tgccggcccg
    10921 ctcccagaat ccggtcgccg tcggtcccgg gcagagcgcg gtcaccgtga ctccgctgcc
    10981 cgcggtctcc tccgccagcg cctcgctgaa cgacaggacg aacgccttcg tcgcgtaata
    11041 caccgccatc ccgggaccgg gaagataggc cgccgtcgag gcgacgttca ggatcctgcc
    11101 ggagcgccgc gccagcatgt cgcgcaggta cggccgggtc agctcggtga ggctggtgac
    11161 gttgagcgcc acgagcgcgg cggtcgcctc ggcgtccgtc tcggcgaatc gccccgagag
    11221 gccggccccg gcgttgttgg cgaggaaatc gaccgggccg acgtcccgct cgacgcgcgc
    11281 gtgcagcgcg gtcgccgccc ccggtcccga cagatcggcc gggacgagcg tcagtgccgc
    11341 ggtcttgacc tccgcctgca cccagcgcgc ggcgcgctgc aggcggcctt catcgcgcgc
    11401 caccatcacg acccgggcga cgtcgcgcgc caggagacgg cagagttcga ggccgatgcc
    11461 gctcgatgct ccggtgacga gagccgtccg cacgcgtccg ccgcccgctg tcgtcatggg
    11521 gccgcgatga tacacgcgga gcgcggcgcc gagtccacgt ccgatccgca ggcgccgtcc
    11581 atccggattg caggggacga tccggtcagg cgcgatgaat cgtcacgcac ccaggcgagt
    11641 cagagtgtcg tgaacgacgg gggtttgccg cacgtgtcgc aatttccggg tgaactgcgt
    11701 ttaggtcgat ggagcgggtc ttcgctccag gagattcgac catgcgacgc gacccccgga
    11761 ttctcgcagc tcttgttctc gtgctcctcg tctccgtcgc aggcggaatg gcggccaccc
    11821 caccggcgcg gggactgcac cccgggctgg atgccaccca cggcgggggc ctcctgccgg
    11881 atggctccca gccgcgcgcc atggcggcgg ccgacttcga cgaagacggt atggccgatg
    11941 tggcggtggc cttttccgac ggccgccgcg gcgtcatcgc gctcctcatc gggaaccgcg
    12001 acgtcgtcga cgcgtcggaa ccggaggccc ggcggcgccg ggcgaaaaat ggcctcgaac
    12061 cgcgcgccct gccgccgttt cgggtcgaag gccctctggt cgatctcccc gcgccggccg
    12121 acctggccgg tgccggcgac ttcgacaacg acggccacct cgacctcgtc gtggcagccg
    12181 aggggggtcg cacgctcctc ctcctcaggg gggacggccg gggcggcttc gccgatccgc
    12241 tgccgcggtc cctccccggc cggctgagtc gcatggtcgt gggcgaaatg aaccatcccg
    12301 attccctggc cgacatcgtc gtggccgtcg agcggcaaca ccagaccgag atcctggtct
    12361 acgaggaccc ggagggggcc tggaaggcgg agccggaatc ggtgcgccgg gggggaccga
    12421 tcacgatgct cgccctcggt gacctgaacg gcgatttcga gcgcgacctc ctcgtcggca
    12481 tcgaccgcgg catcgagatc ctccacggcc gggaccggaa gctggcgctc gggttggggt
    12541 ggcgcgccac ggtggccgcg ccggccctgg agcgtcatcc gctcgactgc acaccccggc
    12601 gcgccgcggt cgggcgcttc ggcggcacga ccggaccgaa tctggccttc ctcgcggacg
    12661 atggcaccgt ccgcctcgcc gcggccggca ccctcgcatc gtcacggatc gtcgcccggg
    12721 ggcggggcgg tcgcctgctc gtcggggcgc gcctcgggga cggagtaggc gacgacctcg
    12781 tgacgattca ggaggacggc tccaggctgc gagcgctcgc gggcctcgac gtccccctcg
    12841 agttcgagcc tctggacgcg ctgtcgatgc ggcttgggcc ggaagcggtc gaatcgctcg
    12901 tcgtcctcaa cggctccggg ccggacctcc tgtcgatcct gccgccggcc gccccgacga
    12961 cctacaccgt caccaccatc gccaacagtg gccccgggtc gctccgcgac gcgatcctga
    13021 gcgccaacac caacgccggc gccgacacca tcgacttcaa catcagtccg agcgcgaacc
    13081 tgacgatcgt gccggcgacg ccactgcccg aggtcaccga tccggtgacg atcgacgcca
    13141 ccacgcagcc gggatacccc gggaccccca tcgtcgagat cgatggcagc ggcccgctgc
    13201 cgcccgggac caacggcctg gtcgtgcgcg ccgggcagag cgtggtgcgc ggtctcgtca
    13261 tccacggctt ctccggcagc ggcgtcctcc tcgacggcgc gggccacaac atcgtcgagg
    13321 ggaacttcat cggcgtcgat gccgccggga cgacggcaat cggcaaccag gactcgggtg
    13381 tggtcatccg cgattcccat ctcaacacga tcggcgggac cgcggccccg gcgcgcaacg
    13441 tcctctccgg caacgcgcag gccggcgtcg cgatccgcgg cccgtccacc ttcgaggcgg
    13501 aac
//
LOCUS       DASRIQ010000472        17976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_723739, whole genome shotgun sequence.
ACCESSION   DASRIQ010000472 DASRIQ010000000
VERSION     DASRIQ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 17976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17976
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723739"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /gene="mutL"
                     /locus_tag="VFD06_11515"
     CDS             complement(<1..1548)
                     /gene="mutL"
                     /locus_tag="VFD06_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZN04199.1"
                     /translation="MGIVRLLPPEVANQIAAGEVVERPASVVKELVENALDAGAARID
                     VRIEGGGADLIEVADDGCGMEPEDATLAIERHATSKVISAADLAAVRSFGFRGEALPS
                     IASVSRLTLTTGAGGEAGTEVRVEAGTLMGTAPAAHPRGTTVRVEGLFHNAPARRKFL
                     RAAGTETAHAIDHLIRLAAAHPGVAFRLTSGGRETLLWPAAAGLRERVAKILGGGEAA
                     LLLDLDRREGRARVRGLVSSPSLTRATARDARLYVNDRPVRDRRLLHAVTEGAATSVP
                     RGRYPVSFLFLDVPAEEVDVNVHPVKSEVRFARPGALHDLVLRAVREALGAARPFAAL
                     GDRPLAEPAAAGDRFALEPSAPDPGPEWTGVRETAAPFAIRPAAPAAPADVPVVPLDR
                     LTPLAQYRDTYILASAPDGLVIVDQHAAHERVLYERLLDDAATSRLERQRLLFPLLLE
                     VSPAERQALDDARDLFSSMGFVLAGFGPGAVRVEEIPGILKATAAESLVRELLADLLD
                     RTRGRAAE"
     gene            complement(1548..2570)
                     /locus_tag="VFD06_11520"
     CDS             complement(1548..2570)
                     /locus_tag="VFD06_11520"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HZN04200.1"
                     /translation="MARPLSFGGRSFPRGAAGQIYLKVGEHATATPMSVPVTIVRGAR
                     PGPMLFLTAAVHGDELNGVEVVRQVMTTVTPEGLRGTLICVPVVNRTGFLAHSRYLPG
                     HQDLNRVFPGRPDGHAAARLAHVLFREIVERCDYGIDLHTAGHGRTNLPHVRARMEDA
                     KVRRLARAFGLEVIVDTPGMSRTLRAAATRAGVPTILFEAGESIRFQRHLVARGVRGV
                     HNVLAAFKMIDAPPRRPRFQIVVKVSEWVRAPRGGIADVQVRPGELIYAGDAVASITN
                     PFGREVSLVRSPVTGLVLGMATVPMVQPGDALCHVAKLHRTLATVERFTLADSRGRRT
                     LLVEGV"
     gene            complement(2575..3483)
                     /locus_tag="VFD06_11525"
     CDS             complement(2575..3483)
                     /locus_tag="VFD06_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HZN04201.1"
                     /translation="MLLAVLSRRRSLYSTRRLLEAAARRGHETIVLDPLQCSLVVGGG
                     APVVTYRGLDGRLPAIDVVLPRIGASITEHGLAVVTQFDMMGLPIVNRAQPIARSRDK
                     LRALQILARAGVAIPKTVMARHPSQVQRALQIVGGPPAILKLVRGTQGIGVILAETEQ
                     AAQQVVETLWSLGMTVLVQEFIEESEGRDVRVLVVGPRAVAAMRRQARLGEFRSNVHR
                     GAAATAVERAPGLERLALKACRALKLDVAGVDVLESRSGPKILEVNSSPGLEGIERAT
                     GKDVASAIVEHAVETARRARRRRQAS"
     gene            complement(3452..6211)
                     /gene="ppdK"
                     /locus_tag="VFD06_11530"
     CDS             complement(3452..6211)
                     /gene="ppdK"
                     /locus_tag="VFD06_11530"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZN04202.1"
                     /translation="MMAVKSVYFFGGGRAEGHGGMKELLGGKGAGLMEMTRIGLPVPA
                     GFVITTASCSRYLKEGRVALKAIDKEVRAHLSRLEKVTGRRFADPDDPLLVSVRSGAA
                     RSMPGMMETILNLGLNDRTVLGLAKKTGNERFAWDAYRRFLGMYAGVVHEVPRSMIEE
                     PFKAVKSRFKVRQDTDVPVEGLKELCGRLKTLYRERTGKDFPQDPTAQLWGAIEAVFR
                     SWNAEKAVTYRRVERITGLAGTAVNVVQMVFGNKGETSGTGVCFTRDPNSGAPVFYGD
                     ALMNAQGEDVVAGIRTPMTLDKLKPILPAVYRQLEEVRRRLERHTRDMQDIEFTIEEG
                     TLYILQTRSGKRSPRAAFRIAADLCREGVITRAEAVQRIAPEDVERLFYPVVDPKVPA
                     ADLAARRLATGIGAVPGAASGALAFTAAEAEERAKAGEAVILVRHETSPEDVGGMHAA
                     QGILTATGGKTSHAAVVARGWGKTCIVGCDAIDPDAQARTLTVAGKVLGPSDKVTLDG
                     STGTVYQGEIPLVQPDLPDAYSTIMRWADGFRRLRVRANVDTPYDAAKAVTMGAEGIG
                     LCRTEHMFFDTEERRLAIQEMILASDREARERALAKLLPLQRKDFEGIFKAMAGRPVT
                     IRLLDPPLHEFVPHDEHGQARLGRALGIPAEAVARRVEQLREANPMLGHRGCRLSLTY
                     PEILEMQVRAILEAAIAAKKKGTKVQPEIMHPLTIDPREMAILVERTRAVADAVVKKA
                     GVRLPYLVGTMIEVPRAALLAGRIAASAEFFSFGTNDLTQMTLALSRDDAGRFLPQYV
                     GEENLGIFADDPFQTLDQDGVGELVRLAIERGRAARPGMKIGVCGEHGGEARSVRFCD
                     AVGMDYVSCSPYRLPIARLAAAQATLAAAPAGKRPRSGSRPRSAVRSRTARIADAPRR
                     TVASA"
     gene            complement(6208..8358)
                     /gene="glyS"
                     /locus_tag="VFD06_11535"
     CDS             complement(6208..8358)
                     /gene="glyS"
                     /locus_tag="VFD06_11535"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZN04203.1"
                     /translation="MSDLRHPFLLEIGTEEIPARMIAAAIEDLARGIGEALATAGLPA
                     RSTRPLGTPRRLAVLVDEVPEQSPDQIVEVTGPPAGVAFDAQGRPTKAGEGFARAQGV
                     RVEDLRRTQGPKGECVAVRKTVPGRRAAAVLADAAPAVAAALAFPKTMRWGSGEHRFV
                     RPVHWIVALYGEEIVPFEFCGVRSGRQTHPHRLKGSEPVSIARASEYTAFLERHGVLV
                     DPQARRQRIAADLAAAAAKAGGRLATPPGSETRGGDADPELLDEVNDLVEWPAILAGE
                     FDPSFLELPQEILLTSMRHHQKYFALVDGAGRLLPRFLAVANVPDDPQGIIRRGHEWV
                     LRARLSDARFFHQEDRKGTLESFSAALERVAFHERLGSYAAKAARMTRLAESLLPAFA
                     AAGHPADAAAVARAASFSKNDLCTQMVGEFPELQGIVGGLYAQADGLPAAVTEAIYDQ
                     YLPQAQDGPLPRGAVGAILSLADRLDTQAGIFLLGLVPTGSRDPYALRRSVQGACRIL
                     VERGVSVALPDLLGSAIAAYPAASLKDAVAPEEALRTLLEFWRGRQEFLAAEAGFPVD
                     VVRAALGAGDGDPADARRRMEAVRDFRKTPGFDDLAAAHKRIRNILAGQKEPEEHFDP
                     AGLKEAAERLLAERIQDTRPLVAAAAGAGDYAGALRRIAALREPVDRFFGDVMVLAED
                     RVLRRNRVALLRDLQALFLTVADFSEIAAHRKEA"
     gene            complement(8355..9245)
                     /locus_tag="VFD06_11540"
     CDS             complement(8355..9245)
                     /locus_tag="VFD06_11540"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZN04204.1"
                     /translation="MNFQQLVLRLSEFWARQGCVVQQPYDVEAGAGTMHPDTFFRVLG
                     PEPWRAAYCAPSRRPVDGRYGQNPFRLYRHYQFQVILKPSPPDVQDLYLASLQAFGIE
                     PNEHDIRFEEDNWESPTLGAAGVGWQVMMDGMEITQFTYFQQAGGQELAPIAAEITYG
                     SERIGMFLNNVASVYDLKWSDHLTYGDVRLQEEIEFSKHNFEAADVELLRTLFQACEK
                     ESARLLQAGLVLPAYDYALRCSHTFNLLEARGAISVTERTGFIQKVRRLAVGCAKEWV
                     KGREAQGWPLLKGAAAGRTL"
     gene            complement(9268..10026)
                     /gene="recO"
                     /locus_tag="VFD06_11545"
     CDS             complement(9268..10026)
                     /gene="recO"
                     /locus_tag="VFD06_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZN04205.1"
                     /translation="MPLRDSEAFVIDSRPLQESDRIVTFLTKSDGKVRTVAKGARRSR
                     KRFGANLMLLSRVRLAWFEKEGRDLARLESADLLESFYDLQADPERGAMLACCAEVTD
                     AFAREQQEDEAFFRLLGATLQAVRQGVDLDRVLRYFEIWTLRLHGVLPDLAACGRCGS
                     TLLARGGTLLRREGIVMCPRCAGERRAGDPRLRPEAATLAAEFMRRPPSALREIEAPP
                     AARESLREIAAALLFDLTDRRFRSYEILARMRQP"
     gene            complement(10048..11403)
                     /gene="mgtE"
                     /locus_tag="VFD06_11550"
     CDS             complement(10048..11403)
                     /gene="mgtE"
                     /locus_tag="VFD06_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZN04206.1"
                     /translation="MKDVTFLVESVKKLLRRGATTHLLNMLTKVRPQDLAEACKTLDE
                     AERNAAFRILVEGHLPTAAGLVQEIHPTLRADLLRGMEPEAVARVLHGLSDDDVAGIV
                     AERPDDEREALLSVMQREASKEVQDLLEYDEETAGRIMTKRYVALPEETLVREAIAAV
                     QKAQEAEMVFYVYVVDDHGRLSGVMSLRRLLTVPPATTLREIMSTDVISVKAETDQEE
                     VARIASRYDLLSVPVVDAHNVLVGIITIDDIVDVMREEATEDFYKLAGTSDEERLLKS
                     TLGAARIRLPWLFAAFAGGLLAALLIKAYTPLLSHAAVLACFIPVVLGMGGNIGTQAA
                     TIMVRGLATGRISVLQAGQVIFKEIRIALLLGLFYGTLLGLAAVPIVGMGAAAGVAIG
                     LSLLASMTMAAVVGAMLPVLLKTMHVDPAVATSPFVTTAVDLLGLLAFFGLASRQLVG
                     G"
     gene            complement(11471..12442)
                     /gene="era"
                     /locus_tag="VFD06_11555"
     CDS             complement(11471..12442)
                     /gene="era"
                     /locus_tag="VFD06_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZN04207.1"
                     /translation="MSDDRDPSGELPAPDAPSAMAGEPPVVPAGFRSGFVALVGRPNV
                     GKSTLLNQMMGELLSIVTDVPQTTRFPVRGIRHLPQAQIVYIDTPGIHKPRHRLNEEM
                     VRAATQVLREVDVVVVLVDASDGYGPGDRFVFERVREAGARAILALNKADAMRKEDLL
                     PLIDQAARLELFQEIVPLSASTGENCDRLLEVILARLPEGPPHFPPETLTDLPFRLRL
                     AEAVRAEILHRTRQEVPHAVAVQIEAVSEEASLTRIDATILVERDSQKGIVIGDGGQR
                     IKEIGTAARRRLEQILGRQVHLALWVKVRPDWRDDEPLLRLLGLAPS"
     gene            complement(12439..12969)
                     /gene="ybeY"
                     /locus_tag="VFD06_11560"
     CDS             complement(12439..12969)
                     /gene="ybeY"
                     /locus_tag="VFD06_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZN04208.1"
                     /translation="MRHLGGPTPPARRRIGPVEVVTRVHGSGRHGAASRRLLLALRRA
                     TRRPASGVTLLLTGDREIRTLNRRHLGHDRVTDVLSFPSEAPLEPGAPHLGDIAVSLP
                     RARRQARRAGWPLRSEIALLLTHGYLHLLGHDHETDDGTMHRLEARLLERIAGVRLDR
                     RRLPWGTPPRGPRGRR"
     gene            complement(12969..14000)
                     /locus_tag="VFD06_11565"
     CDS             complement(12969..14000)
                     /locus_tag="VFD06_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZN04209.1"
                     /translation="MRRFSIPEVTLADVFGHFDEHLKSIEKELEVQIAARGAEISVSG
                     DPEKEALAANLMTQLAALVQGGTPIKKGDVEIAIRLLRDNRSALLKDFFAAGRLRTTN
                     GRFVTPKSPNQKALLDAIQYHDLVFVIGPAGTGKTFLAVAAAVAHLAEKKVERIILAR
                     PAVEAGEKLGFLPGDMAQKVDPYFRPLYDALYQLIDRDRANGHVERGTIEIAPLAFMR
                     GRTLSNSFVILDEAQNTTSEQMKMFLTRLGHNSKALITGDVTQVDLPPGRTSGLVEAR
                     RVVAGVEGVRFIEFDETDVVRHPLVQGIVRAYEAYREAGGEAAVEPPPTEAAPLRRRR
                     RAAGDPAGG"
     gene            complement(14115..15911)
                     /gene="aspS"
                     /locus_tag="VFD06_11570"
     CDS             complement(14115..15911)
                     /gene="aspS"
                     /locus_tag="VFD06_11570"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZN04210.1"
                     /translation="MVDAPVWRRTHTCGALRPSDAQHEAVLLGWVDSIRDHGGLLFAD
                     LRDRYGVTQAVFHPESQPQAFAAAKDLRPGFVVAVAGKVVARDPQNVNAGLPTGAVEV
                     QAARLEVLNTSEPLPFPLDDEANVAEEVRLRYRFLDLRRRKSQQIFETRHRILMAVRR
                     YLDAQSFLEVETPILNRSTPEGSREYLVPARVSPGHFYSLPQSPQLFKQLLMVAGFDR
                     YFQIAKCFRDEDSRADRQPEFTQIDVEVSFPERESFFALIEGLMEEIFRAAGAKLQGP
                     YPRMSYREAMERFGSDKPDTRFGVEIRDLTTHLKDSSFRVFSDAVKQGGVIKGLVVPG
                     GAAWGRQRIDNLVDAAKGMGSKGLLWMRLSGGAVQSPLAKHLGEEGCRAVLATLEAGP
                     DDLLLIVADRFKPALSVLGALRLLVSRAEKWIDPAKHHMLWVHEFPLFEHSQDEGRLV
                     ACHHPFTSPMPEDVPLLETSPGDARALAYDLVVDGNEIAGGSVRIHRSEVQEQVFRVL
                     GMDRQESEHRFGFLLSALRMGAPPHGGIAFGVDRLVALLTGSPSIRDVIAFPKTTSGL
                     DLMTGAPAEVPAKQLRELHVKIDLPPKGAPGS"
     gene            complement(15904..17163)
                     /gene="hisS"
                     /locus_tag="VFD06_11575"
     CDS             complement(15904..17163)
                     /gene="hisS"
                     /locus_tag="VFD06_11575"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZN04211.1"
                     /translation="MIEAVKGTRDILPAEAGRWQFVEAQAREVFERYGFREIRTPIFE
                     PTELFARGVGEGTDIVAKEMYTFLDRKGRSLTLRPEGTAPVARAYLQHGMQRGSDLTR
                     LYYIGPMFRYERPQKGRMRQFAQIGVEALGSEHPAVDAETLHMLSVFLDRLGLSRIEL
                     LLNSVGCENCRPAYREILKAELRPFRDRLDADCQRRLETNPLRVFDCKVPEDQALIAQ
                     VSPILDHLCDDCRRHLEGVRGHLERLGVPYTMAPRLVRGLDYYRRTTFEVTLPTLGAQ
                     SALLGGGRYDGLVQELGGPPVPGFGFAVGEDRLVMSLPEDRAEGERPPDAVFVILGEE
                     GVPAALEGAKRLREAGLSAVVEPFPDRSAKAQLRRANDLKAKMAILVGSSEVAAGALT
                     VKRLQDGLQKTIAAADVASSVREMLHG"
     gene            17282..>17976
                     /locus_tag="VFD06_11580"
     CDS             17282..>17976
                     /locus_tag="VFD06_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN04212.1"
                     /translation="MRLSRGRSRRLVAAVVTILILPVVPPVDARDAKSVFSGLPIDPD
                     MAKPAEPEHPILFEPAWRFDGFSAPLAGDLAVSETLLAGVDVAGRLIALDPKEGRERW
                     RVDLEAAAAVGPALSDERVLQGTSDGRLQAFAASDGRPLWTADLGAAPAVAAVAIAGT
                     ILQATRAPEVLAINPEDGTVTARLALESIPLPPVEARLKDGAVEAVIAAEEGQIRLVE
                     PGSLTVRWRRALG"
BASE COUNT         2581 a         6449 c         6408 g         2538 t
ORIGIN      
        1 ctccgcagcc cggccgcggg tgcgatcgag caggtcggcg agcagctccc gcacgaggct
       61 ctcggcggcg gtcgccttga ggatgccggg gatctcctcg acccggaccg cccccggtcc
      121 gaacccggcc aggacgaacc ccatggagga gaacagatcg cgcgcgtcgt cgagcgcctg
      181 gcgctccgcc ggggagacct ccaggagcag cgggaagagg agccgctggc gctccagccg
      241 gctggtcgcg gcgtcgtcga ggagacgctc gtacaacacc cgctcgtgcg ccgcgtgctg
      301 atcgacgatc accagcccgt cgggggcgga ggcgaggatg taggtgtcgc ggtactgcgc
      361 gagaggcgtc aggcgatcga gcggcaccac cgggacgtcc gccggtgccg ccggcgccgc
      421 ggggcgaatg gcgaatgggg cggccgtctc ccgcacgccg gtccattccg gtccgggatc
      481 gggggccgag ggctccaggg cgaaccgatc gcccgccgcg gcgggctccg ccagcggccg
      541 gtccccgagc gccgcgaacg gccgcgccgc gccgagcgct tcgcgcaccg cgcgcaggac
      601 gagatcgtgc agcgcccccg gccgtgcgaa gcggacctcg ctcttgaccg gatggacgtt
      661 cacgtccacc tcctccgccg gcacgtcgag aaacaggaag ctgaccgggt aacgtccgcg
      721 cggcaccgac gtggccgccc cctcggtgac ggcgtgcagg aggcggcgat cgcgcaccgg
      781 gcgatcgttg acgtagagcc gcgcgtcgcg cgccgtggcg cgcgtcaggg agggggacga
      841 gaccagcccg cggacgcggg cccggccttc gcgccgatcg aggtcgagga ggagcgccgc
      901 ctcgccgccg ccgaggatct tcgccacgcg ctcccggagc cccgcggccg caggccacag
      961 gagggtctcg cgtccgccgc tcgtgagccg gaaggcaacg cccggatggg cggcggcgag
     1021 gcggatgagg tgatcgatcg cgtgcgccgt ctcggttccg gcggcgcgga ggaacttgcg
     1081 ccgcgccggg gcgttgtgga acagcccctc gacccgcacc gtcgtgccgc gcgggtgggc
     1141 ggcgggggcg gtccccatga gcgtccccgc ctcgacccgc acctcggtgc cggcctcccc
     1201 tcccgccccc gtcgtcagcg tcagccggga gaccgaggcg atcgacggca gcgcctcgcc
     1261 gcggaagccg aaggaccgta ccgcggcgag atccgcggcg gagatcacct tgctcgtggc
     1321 gtggcgctcg atcgccaggg tcgcgtcctc cggctccatg ccgcagccgt cgtcggccac
     1381 ttcgatgagg tcggcgccgc ccccttcgat ccggacatcg atgcgcgccg cgccggcgtc
     1441 gagcgcgttc tcgaccagct ccttgacgac cgaggcgggc cgctcgacga cttccccggc
     1501 ggcgatctgg ttcgccacct cgggcggaag gaggcggacg atccccatca caccccctcg
     1561 acgagcagcg tccggcgccc gcgcgagtcg gcgagggtga accgttcgac ggtggcgagg
     1621 gtgcggtgca gcttggcgac gtggcacagc gcgtcccccg gctgcaccat cgggaccgtc
     1681 gccatcccga ggacgaggcc ggtgaccggc gagcgcacca gcgacacctc gcgcccgaac
     1741 gggttggtga tcgacgccac cgcgtccccg gcgtagatca gctctccggg acgcacctgc
     1801 acgtccgcga tgccaccgcg cggggcccgg acccactccg acaccttcac gacgatctga
     1861 aagcgcggcc ggcgcggcgg ggcgtcgatc atcttgaagg ccgccaggac gttgtggacc
     1921 ccgcgcacgc cgcgcgccac caggtgccgc tggaagcgga tcgactcccc cgcctcgaag
     1981 aggatcgtcg ggacgccggc ccgggtggcg gcggcgcgca gcgtacgcga catccccggc
     2041 gtgtcgacga tcacctcgag gccgaaggcg cgtgccagac ggcgcacctt ggcgtcctcc
     2101 atccgcgccc gcacgtgtgg caggttggtg cgcccgtggc cggccgtgtg caggtcgatg
     2161 ccgtagtcgc agcgctcgac gatctcgcgg aacagcacgt gcgccaggcg ggcggcggcg
     2221 tggccgtcgg ggcgcccggg gaagacgcgg ttcagatcct ggtgtccggg caggtagcgc
     2281 gagtgggcga ggaatccggt gcggttcacg accgggacgc agatgagcgt cccgcgcagc
     2341 ccttccgggg tgacggtggt catcacctgc cgcaccacct ccacaccgtt gagttcatcg
     2401 ccgtggaccg ccgccgtcag gaagagcatc ggcccgggcc gcgccccgcg cacgatggtc
     2461 acagggacgc tcatcggcgt cgccgtggcg tgctccccga ccttgagata gatctgcccc
     2521 gccgcgccgc gcgggaacga tcgcccgccg aaggacaggg gacgggccat ctcctcagga
     2581 ggcctgtcgg cgacggcggg cccggcgcgc cgtctcgacc gcgtgctcga cgatcgccga
     2641 ggcgacgtcc ttgccggtgg cccgctcgat cccctcgagc cccggggagg agttcacctc
     2701 gaggatcttc ggcccggagc gggactcgag cacgtcgacg ccggcgacgt cgagcttgag
     2761 cgcgcggcag gctttgagcg ccagccgttc gagccccggg gcacgctcca ccgcggtggc
     2821 ggcggcgccg cgatggacgt tcgatcggaa ctccccgagg cgcgcctggc ggcgcatggc
     2881 ggcgaccgcg cggggcccga ccaccagcac ccgcacgtcg cgaccctccg attcctcgat
     2941 gaactcctgc acgagcacgg tcatcccgag gctccagagg gtctcgacga cctgctgggc
     3001 cgcctgctcg gtctccgcga ggatcacccc gatcccctgc gtcccgcgca ccagtttcag
     3061 gatcgccggg gggccgccga cgatctgcag ggcgcgctgc acctgggagg ggtggcgggc
     3121 catgacggtc ttcgggatgg cgacgccggc ccgcgccagg atctgcaggg cgcgcagctt
     3181 gtcgcgcgat cgcgcgatcg gctgcgcccg gttgacgatc ggcagcccca tcatgtcgaa
     3241 ctgcgtcacc accgccagtc cgtgctcggt gatcgacgcg ccgatgcggg gcagcaccac
     3301 gtcgatcgcc ggcagtcggc cgtcgaggcc gcggtaagtg acgaccggcg ccccgccgcc
     3361 gacgacgagg gagcactgca gcggatcgag gacgatcgtc tcgtggccgc gccgcgccgc
     3421 cgcctccagg aggcgccggg tggagtagag gctacgccga cgcgacagta cggcgaggag
     3481 catctgcgat cctggcggtg cgcgaccgca cggcggagcg cggccgggag ccggaacggg
     3541 gacgcttccc cgccggcgcg gctgcgagcg tggcctgcgc ggccgcgagt cgggcgatcg
     3601 gcagccggta gggggagcag gagacgtagt ccatcccgac cgcgtcgcag aagcggacgg
     3661 agcgcgcctc cccgccgtgc tcgccgcaga cgccgatctt catcccgggc cgggcggcgc
     3721 ggccgcgctc gatcgcgagg cgcaccaact caccgacgcc atcctggtcg agcgtctgga
     3781 acggatcgtc ggcgaagatt ccgaggttct cctcgccgac gtactgcggc aggaagcgcc
     3841 ccgcgtcgtc gcgcgacagc gccagtgtca tctgcgtcag gtcgttggtg ccgaaggaga
     3901 agaactcggc cgaggcggcg atgcgaccgg cgaggagcgc cgcccgcggc acctcgatca
     3961 tcgtgccgac gaggtacggc agccggacgc ccgccttctt cacgaccgcg tcggcgaccg
     4021 cccgcgtccg ctccaccagg atcgccatct cacgcgggtc gatggtgagc gggtgcatga
     4081 tctccggctg gactttcgtc cccttctttt tcgccgcgat cgccgcctcg aggatggccc
     4141 gcacctgcat ctcgaggatt tccgggtagg tgagggagag ccggcaaccc cggtgcccga
     4201 gcatcgggtt cgcctcgcgc agctgctcga cgcgccgcgc caccgcctcc gccggaatcc
     4261 cgagggcgcg gccgagacgc gcctgcccat gctcgtcgtg cggcacgaac tcgtggagcg
     4321 gcgggtcgag cagccggatg gtgaccggcc ggcccgccat cgccttgaag atcccctcga
     4381 aatccttgcg ctgcagcggg aggagcttgg cgagggcgcg ctcccgcgcc tcacgatcgg
     4441 aggcgaggat catctcctgg atcgccaggc gccgctcctc ggtgtcgaag aacatgtgct
     4501 cggtgcggca gagcccgatc ccctccgccc ccatcgtcac cgccttcgcg gcatcgtacg
     4561 gggtgtcgac gttcgcccgc acgcggagcc ggcggaaccc gtcggcccag cgcatgatcg
     4621 tgctgtaggc gtccgggagg tcgggctgca ccagcgggat ctccccctgg tacacggtgc
     4681 cggtcgagcc gtcgagcgtg accttgtcgc tcgggcccag cacctttccg gcgaccgtga
     4741 gggtccgggc ctgcgcgtcg ggatcgatgg cgtcgcagcc gacgatgcag gtcttgcccc
     4801 agccgcgcgc cacgaccgcg gcgtgggacg tctttccccc ggtcgcggtg agaatcccct
     4861 gcgccgcgtg catgccgccg acgtcctcgg gggaggtttc gtgccggacg aggatgactg
     4921 cctcgcccgc tttcgcccgc tcctccgcct cggcggcggt gaaggcgagc gctcccgagg
     4981 cggcgccggg gacggcgccg atcccggtcg cgagccggcg cgccgccaga tcggcggccg
     5041 gaaccttggg atcgacgacg ggatagaaga gacgctcgac gtcctccggg gcgatccgct
     5101 gcaccgcttc ggcccgggtg atgaccccct cgcggcacaa gtcggcagcg atccggaagg
     5161 cggcgcgcgg cgatcgcttg ccggagcgcg tttgcaggat gtaaagggtc ccttcctcga
     5221 tggtgaactc gatgtcctgc atgtcgcgcg tgtgccgctc gagccggcgc cggacctcct
     5281 ccagctgccg gtacacggcc gggaggatcg gcttcagctt gtcgagggtc atcggcgtgc
     5341 ggatgccggc caccacgtcc tccccctggg cgttcatcag ggcgtcgccg tagaagaccg
     5401 gcgcgccgct gttcgggtcg cgcgtgaagc agacgcccgt gccggacgtc tcccccttgt
     5461 tgccgaacac catctgcacg acgttcaccg ccgtgccggc cagcccggtg atccgctcga
     5521 cgcgccggta ggtcaccgcc ttttcggcgt tccaggaccg gaacaccgcc tcgatcgccc
     5581 cccacagctg cgccgtcggg tcctgcggga agtctttccc cgtccgctcc cggtacaggg
     5641 tcttcagccg gccgcagagc tccttcagcc cttcgaccgg cacgtcggtg tcctggcgga
     5701 ccttgaaacg gcttttcacg gccttgaacg gctcctcaat catcgagcgc ggcacctcgt
     5761 ggacgacacc ggcatacatc cccagaaaac gccggtacgc gtcccacgcg aagcgctcgt
     5821 tgccggtctt cttcgcgagc ccgagcacgg tgcgatcgtt gaggccgagg ttgaggatcg
     5881 tctccatcat cccgggcatc gaccgggcgg cgccggagcg gaccgagacc aggagcgggt
     5941 cgtccggatc ggcgaagcgc ctgccggtca ctttctcgag gcggctcagg tgcgcgcgca
     6001 cttccttgtc gatcgccttg agcgcgacgc ggccctcctt gagataacgg gagcagctgg
     6061 cggtggtgat gacgaacccg gccgggacag gaagcccgat gcgggtcatc tccatgagtc
     6121 ccgccccctt gccgccgagg agctccttca tgccgccgtg cccctcagct cggccgccgc
     6181 cgaaaaagta gaccgacttc actgccatca ggcctccttg cggtgcgcag cgatctcgct
     6241 gaagtccgcc acggtgagga acaacgcctg gagatcgcgc agcagcgcca cgcggttccg
     6301 gcgcaacacc cgatcctccg ccagcaccat cacgtccccg aagaagcgat cgaccggctc
     6361 gcgcagcgcc gcgatccggc gcagcgcccc ggcatagtcc ccggcgccgg ccgccgcggc
     6421 gacgaggggg cgcgtgtcct ggatgcgctc ggccaggagg cgctccgctg cttccttcaa
     6481 cccggccgga tcgaagtgct cctccggctc cttctgcccg gcgaggatat tgcggatgcg
     6541 cttgtgcgcc gctgcgaggt cgtcgaagcc cggggtcttc cggaagtcgc gcaccgcttc
     6601 catgcggcgg cgcgcgtcgg ccggatcgcc gtcccccgcg ccgagcgccg cccggaccac
     6661 gtccaccggg aagccggcct cggcggccag gaactcctgt cggccgcgcc agaactcgag
     6721 aagggtacgg agggcctcct ccggtgcgac ggcgtccttg aggctggccg ccgggtacgc
     6781 ggcgatcgcc gaccccagga gatccggaag cgcgacgctc acgccccgct ccaccaggat
     6841 ccgacaggcc ccctggacgg agcgccgcag ggcatacgga tcgcgcgagc cggtcggcac
     6901 cagccccagg aggaagatgc cggcctgcgt gtcgagccgg tcagcgagcg acaggatcgc
     6961 cccgacggcg ccgcgcggca gcggcccgtc ctgcgcctgc ggcaggtact ggtcgtagat
     7021 cgcctcggtg accgcggcag gaagaccgtc cgcctgggcg tacagtccgc cgacgatccc
     7081 ctggagctcc gggaactccc cgaccatctg cgtgcacagg tcgttcttgg agaaggatgc
     7141 ggcccgtgcg accgccgcgg cgtccgccgg gtgacccgcg gcggcgaacg ccggcaggag
     7201 cgactccgcc aggcgggtca tgcgcgccgc tttggcggca tacgagccga ggcgctcgtg
     7261 gaacgccacc cgctcgagcg ccgcggaaaa cgactcgagc gtccccttgc gatcctcctg
     7321 gtggaagaag cgggcgtcgg agagtcgcgc ccgcagcacc cactcgtgac cgcgccggat
     7381 gatcccctgg gggtcgtccg gcacgttggc gacggcgagg aagcgcggca ggagccgccc
     7441 ggccccgtcc acgagcgcga agtacttctg atggtgccgc atactggtga gcaggatctc
     7501 ctgaggcagc tccaggaagg acggatcgaa ttcccccgcg aggatcgcag gccattccac
     7561 gagatcgttg acctcgtcga ggagctccgg gtcagcgtcg ccgccgcgcg tctcgcttcc
     7621 gggcggggtc gcgaggcgcc ctcccgcctt cgccgcggcc gctgcgagat cggcggcgat
     7681 tcgctggcgg cgcgcctgcg gatcgacgag caccccatgg cgctcgagaa acgccgtgta
     7741 ctcggaggcg cgcgcgatgg acaccggttc gctccccttc aatcggtggg ggtgcgtctg
     7801 ccgcccggag cggacgccgc aaaattcgaa cggcacgatc tcctcgccgt acagcgcgac
     7861 gatccagtgg accggccgga cgaagcggtg ctcgccgctc ccccaacgca tcgtcttcgg
     7921 gaacgcgagg gccgcggcga ccgccggcgc cgcgtccgcc aggaccgcgg cggcgcgccg
     7981 tcccgggacc gtcttccgga cggcgacgca ctcccccttg gggccctggg tgcggcgcag
     8041 gtcctcgacc cgcaccccct gtgcgcgcgc gaacccttct cccgctttcg tcgggcgccc
     8101 ctgggcgtcg aacgcgacgc ccgccggcgg tccggtcacc tccacgatct ggtccggcga
     8161 ctgctcgggc acctcgtcga ccaggaccgc cagacgccgc ggcgtcccca ggggccgggt
     8221 ggacctggcc gggagcccgg ccgtcgcgag ggcctcgccg atgccgcgcg ccagatcctc
     8281 gatcgcggcg gcgatcatcc gggccgggat ctcctcggtg ccgatctcca gcaggaacgg
     8341 gtggcggagg tcgctcacaa ggtcctgcct gcggcggcgc ccttgagcag cggccagccc
     8401 tgcgcctcgc gccctttcac ccattccttc gcgcagccga cggcgaggcg gcgcaccttc
     8461 tggatgaatc cggtcctttc ggtgaccgag atggcgccgc gcgcctcgag caggttgaag
     8521 gtgtgcgagc agcgcagcgc gtagtcgtac gccgggagca ccaggccggc ctgcaggaga
     8581 cgcgccgatt ccttctcgca ggcctggaaa agcgtccgga ggagctcgac gtcggccgcc
     8641 tcgaagttgt gcttcgagaa ctcgatctcc tcctggagcc ggacgtcgcc gtaggtcagg
     8701 tgatcgctcc acttcaggtc gtagacgctc gcgacgttgt tgaggaacat gccgatgcgc
     8761 tccgagccgt aggtgatctc ggcggcgatc ggagccagct cctgaccgcc cgcctgctgg
     8821 aagtaggtga actgggtgat ctccatcccg tccatcatga cctgccagcc gacgccggcc
     8881 gcgccgagcg tgggcgactc ccagttgtcc tcctcgaagc ggatgtcgtg ctcgttcggt
     8941 tcgatgccga acgcctgcag cgacgcgagg tacaggtcct gcacgtcggg cggggacggc
     9001 ttgaggatca cctggaactg gtagtgccgg tagaggcgga acgggttctg gccgtagcgg
     9061 ccgtccacgg ggcggcgcga gggggcgcag taggcggcgc gccagggctc cggacccagg
     9121 acgcggaaga acgtgtccgg gtgcatggtg ccggcccccg cctcgacgtc gtacggctgc
     9181 tgcacgacgc acccctgccg ggcccagaac tcgctcaggc gcagcaccag ctgctggaag
     9241 ttcaggccca ccggttcccc cttcggatca cggctgccgc atccgcgcca ggatctcgta
     9301 gctccggaag cgccggtcgg tcagatcgaa gagcaacgcc gcggcgatct cccggagcga
     9361 ttcgcgggcc gcggggggcg cctcgatctc ccggagcgcc gacggcgggc gccgcatgaa
     9421 ctcggcagcc agtgtggcag cctccggacg gaggcgcggg tcgccggcgc gccgctcccc
     9481 cgcgcagcgg gggcacatga cgatcccctc gcggcgcagg agcgtcccgc cgcgggccag
     9541 aagcgtcgac ccgcagcggc cgcaggccgc gagatcgggg agcacaccgt gcagacgaag
     9601 cgtccagatc tcgaagtatc gcagcactcg atcgaggtcg accccctgcc gtaccgcctg
     9661 cagcgtcgcc ccgaggaggc ggaagaacgc ctcgtcctcc tgctgctcgc gggcaaaggc
     9721 gtcggtcacc tcggcgcagc atgcgagcat cgcgccccgt tccgggtcgg cctgcaggtc
     9781 gtagaacgac tcgaggagat cggccgactc gaggcgggcg agatcgcgcc cctccttctc
     9841 gaaccaggcg aggcgcaccc gcgacagcag catcagattg gcaccgaacc gtttgcggct
     9901 gcggcgcgcc cccttcgcga ccgtccgcac cttgccgtcg cttttcgtca ggaacgtcac
     9961 gatgcggtcg gattcctgca acggacggct gtcgatgacg aacgcctcgc tgtcccggag
    10021 cggcaatcga tccctcccgc gcgtcgatca tccgcccacg agctgccggc tggccagtcc
    10081 gaagaaggcg aggaggccga ggaggtccac ggcggtggtg acgaaggggc tggtggccac
    10141 cgccggatcg acgtgcatcg tcttcaggag caccggcagc atggcgccga ccaccgccgc
    10201 catggtcatc gacgcgagga gcgacagtcc gatggcgacg ccggcggcgg cccccatgcc
    10261 gacgatcggc acggccgcga gcccgaggag ggtgccgtag aagaggccga gcaggagcgc
    10321 gatgcggatc tccttgaaga tcacctgacc cgcctgcagc accgagatgc gccccgtggc
    10381 gaggccgcgc accatgatgg tggcggcctg ggttccgatg tttcccccca tcccgaggac
    10441 gaccgggatg aagcaggcca ggaccgccgc gtgggagagg agcggcgtgt aggccttgat
    10501 caggagcgcc gccaggagcc ccccggcgaa cgccgcgaag agccacggca gccggatgcg
    10561 cgccgccccc agggtcgact tcagcagccg ctcctcgtcg gaggtgccgg cgagcttgta
    10621 gaagtcctcg gtcgcctcct cgcgcatgac gtcgacgatg tcgtcgatgg tgatgatgcc
    10681 gacgaggacg ttgtgggcgt ccaccaccgg cacgctcagg aggtcgtagc gcgaagcgat
    10741 gcgcgcgacc tcctcctggt cggtttccgc cttcaccgag atgacgtcgg tcgacatgat
    10801 ctcgcgcagc gtcgtcgccg gcggcacggt cagaaggcgc cgcagcgaca tcacgcccga
    10861 gaggcggccg tggtcgtcca cgacgtagac gtagaagacc atctccgcct cctgcgcctt
    10921 ctgcacggcg gcgatcgcct cccgcaccag cgtctcctcg ggaagcgcca cgtagcgctt
    10981 ggtcatgatg cggccggcgg tttcctcgtc gtactccagg aggtcctgga cctccttcga
    11041 ggcctcgcgc tgcatcaccg acagcagggc ctcgcgctcg tcgtcggggc gctcggcgac
    11101 gatgccggcg acgtcgtcgt ccgacaggcc gtgcagcacg cgcgccaccg cctccggctc
    11161 catgccccgg agaaggtcgg cgcgcagagt cggatggatc tcctgcacca ggcccgccgc
    11221 ggtcgggagg tgcccctcca cgaggatgcg gaaggccgcg ttccgctcgg cctcgtcgag
    11281 cgtcttgcag gcctcggcga ggtcctgggg cctgaccttg gtcagcatgt tgagcagatg
    11341 ggtggtggct ccccggcgga ggagcttctt caccgactcc accaggaacg tgacgtcctt
    11401 catggcgccc ccgaagcggg cgccattcta gcccgattcg atcggattcc cggcgcgttc
    11461 gagggcggcg ttacgagggg gcgaggccga ggaggcgcag gagcggctcg tcgtcgcgcc
    11521 agtcggggcg gaccttcacc cacagcgcga ggtgcacctg ccggccgagg atctgctcga
    11581 gacgccggcg ggcggcggtg ccgatctcct tgatccgctg ccctccgtcg ccgatgacga
    11641 tccccttctg gctgtcgcgc tcgacgagga tggtcgcatc gatgcgggtc aaggacgcct
    11701 cctcggagac cgcttcgatc tgcacggcga cggcgtgcgg cacctcctgg cgcgtgcggt
    11761 gcaggatctc cgcccggacc gcctccgcga ggcggagccg gaacggcaga tcggtcagcg
    11821 tctcgggcgg gaaatgcggc ggcccctccg gcagccgggc gaggatcacc tcgagcagcc
    11881 ggtcgcagtt ctcgccggtg gaggccgaca gcggaacgat ctcctggaac agctcgaggc
    11941 gcgccgcctg gtcgatgagc ggcagcaggt cctccttgcg catggcgtcc gccttgttga
    12001 gcgccaggat cgcacgcgcc cccgcctccc gcacccgctc gaagacgaag cgatcgccgg
    12061 ggccgtagcc gtccgaggcg tccaccagca ccaccaccac gtccacctcg cgcagcacct
    12121 gggtggccgc ccgcaccatc tcctcgttca ggcggtggcg cggcttgtgg atgccgggag
    12181 tgtcgatgta gacgatctgc gcctggggca ggtggcggat gccgcgcacc gggaaacggg
    12241 tggtctgcgg cacgtcggtg acgatcgaca ggagctcgcc catcatctgg ttcaggaggg
    12301 tcgacttccc gacgttcggc cgccccacga gcgccacgaa cccggagcgg aatccggcgg
    12361 ggacgacggg cggctctccg gccatggcgg agggcgcgtc cggcgcaggc agctcgccgc
    12421 tcgggtcgcg atcgtcgctc atcttcgacc tctggggcct ctgggcggcg tcccccacgg
    12481 cagccggcgg cggtcgagcc tcacgccggc gatccgttcc aggagcctcg cctcgagacg
    12541 gtgcatcgtc ccgtcgtcgg tttcgtggtc gtgtccgagc aggtgcaggt agccgtgcgt
    12601 caggaggagc gcgatctccg atcgcagcgg ccagccggcg cggcgtgcct ggcggcgggc
    12661 ccgcggcagc gagacggcga tgtcccccag gtgcggtgcg cccggctcga gcggcgcctc
    12721 cgaggggaac gacaggacgt cggtgacgcg gtcgtgcccg agatgcctgc ggttgagcgt
    12781 gcggatctcc cggtcgccgg tgagcagcag ggtcactccg gaggcggggc ggcgcgtcgc
    12841 gcgcctcagg gccaggagca gccgccggct ggcggctccg tgccggcccg agccgtggac
    12901 gcgggtgacg acctcgaccg gaccgatccg ccgcctcgcc gggggggtcg gccccccgag
    12961 atgacgcatc agccgcccgc gggatcgccg gcggcccgcc gccggcgtcg cagcggtgcg
    13021 gcctccgtgg gtggaggctc caccgcggcc tcgccccccg cctcgcggta cgcctcgtag
    13081 gcgcgcacga tcccctgcac cagcgggtgg cggacgacgt cggtctcgtc gaactcgatg
    13141 aagcggaccc cttcgacgcc ggcgacgacg cggcgcgcct ccaccaagcc cgaggtgcgg
    13201 ccggggggca ggtcgacctg cgtcacgtcg ccggtgatga gcgccttcga attgtggccg
    13261 aggcgggtca ggaacatctt catctgctcg gaggtggtgt tctgcgcctc gtcgaggatg
    13321 acgaaggaat tcgacagggt ccggccgcgc atgaaggcga gcggcgcgat ctcgatcgtg
    13381 ccgcgctcga cgtgcccgtt ggcgcgatcc cgatcgatca gctggtagag ggcgtcgtag
    13441 agcggccgga aatagggatc caccttctgc gccatgtcgc ccgggaggaa gccgagcttc
    13501 tcccccgcct cgaccgccgg ccgcgccagg atgatccgct cgaccttctt ttccgccagg
    13561 tgtgcgaccg cggcagcgac ggcgaggaac gtcttgccgg tgccggcagg gccgatgacg
    13621 aacaccaggt cgtggtactg gatggcgtcg aggagcgcct tctggttcgg gctcttcggg
    13681 gtgacgaacc ggccgttggt cgtccggagc cggcccgcgg cgaaaaaatc tttcaggagc
    13741 gcggagcggt tgtcgcgcag gaggcggatg gcgatctcga cgtccccctt cttgatgggg
    13801 gtccccccct gcaccagcgc cgccagctgg gtcatgaggt tggcggcgag cgcctccttc
    13861 tccggatcgc ccgagacgct gatctcggcg ccgcgcgcgg cgatctggac ctccaactcc
    13921 ttttcgatgc tcttgaggtg ttcgtcgaaa tgaccgaaga cgtcggcgag ggtgacttca
    13981 gggatggaga agcggcgcaa ccgatccctc cgaaaacagc gcaaaggtag catccggctc
    14041 ccccgttgtc aatgaaaacg gagcggcgga tcgggcccaa gcgccgcagg ggcaggcgtt
    14101 tgcggagaaa ctgatcagct tcctggggcg cccttcggag gcaggtcgat cttcacgtgg
    14161 agctcccgga gctgcttcgc ggggacctcc gccggcgcgc cggtcatcag gtccaggccg
    14221 ctcgtggtct tcggaaaggc gatgacatcc cggatcgacg gggagccggt gagcagggcg
    14281 acgagacggt cgaccccgaa ggcgatgccc ccgtgcggtg gagcccccat gcgcaacgcc
    14341 gacaggagga agccgaaacg atgctccgac tcctgacgat ccatccccag cacgcggaag
    14401 acctgctcct gcacctcgct gcggtggatt cggacgctcc ccccggcgat ctcgttgccg
    14461 tcgacgacga ggtcgtacgc cagggcccgg gcgtcgccgg ggctggtctc gaggagcggc
    14521 acgtcttccg gcatcgggga ggtgaacggg tgatggcagg cgacgagccg cccctcgtcc
    14581 tggctgtgct cgaacaaggg gaactcgtgc acccagagca tgtggtgctt cgccgggtcg
    14641 atccacttct ccgcccttga gaccagaagc cgcagcgcgc cgaggacgct gagggccggc
    14701 ttgaagcggt cggcgacgat gagcagcagg tcgtccgggc cggcctcgag cgtcgcgagg
    14761 acggcgcggc agccctcctc tccgaggtgc ttcgcgagcg gcgactgcac cgcgccgccg
    14821 gagaggcgca tccacaggag ccccttcgag cccatcccct tcgccgcgtc gacgaggttg
    14881 tcgatccgct gtcgtcccca ggcggccccg cccggcacga ccagcccctt gatcacgccc
    14941 ccctgcttga cggcgtcgga gaagacgcgg aaggagctgt ccttcaggtg ggtcgtgagg
    15001 tcgcggatct cgacgccgaa gcgggtgtcc ggcttgtcgc tcccgaaacg ctccatcgcc
    15061 tcccggtacg acatgcgggg gtacgggccc tgaagcttcg cgccggcggc gcggaaaatc
    15121 tcctccatca gcccttcgat cagcgcgaag aaggactccc gctccgggaa cgacacctcg
    15181 acgtcgatct gggtgaactc cggctgacga tcggcgcgcg agtcctcgtc gcggaagcac
    15241 ttggcgatct ggaagtagcg gtcgaagccg gcgaccatca ggagctgctt gaagagctgc
    15301 ggcgactgcg gcagcgagta gaaatgtccc gggctgaccc gcgctggcac caggtactcg
    15361 cggcttcctt ccggtgtgct gcggttcagg atcggggtct ccacctcgag gaacgactgc
    15421 gcgtcgagat agcggcgcac cgccatcagg atccggtgcc gggtctcgaa gatctgctgg
    15481 gacttccggc gccgcagatc gaggaagcga tagcgcaggc gcacctcctc cgcgacgttc
    15541 gcctcgtcat cgagcgggaa cggcagcggc tccgaggtgt tgagcacctc gagacgcgcc
    15601 gcctgcacct cgacggcgcc ggtgggcagc cccgcgttga cgttctgcgg atcgcgggcc
    15661 acgaccttcc ccgcgaccgc cacgacgaag ccgggccgga gatccttcgc ggcggcgaac
    15721 gcctgcggct gcgattccgg atggaagacc gcctgggtga cgccgtagcg atcgcgcaga
    15781 tcggcgaaca ggagtccgcc gtgatcccgg atgctgtcga cccagccgag gagcaccgcc
    15841 tcgtgttgcg catccgaggg gcggagcgcc ccacaggtat gagttcgccg ccagaccggc
    15901 gcgtcaacca tggagcatct cccggacgga ggaggcgacg tcggccgcgg cgatcgtctt
    15961 ctgcagcccg tcctggaggc gcttcacggt gagcgccccg gcggccacct cgctgctgcc
    16021 gaccaggatc gccatcttcg ccttcaggtc gttggcgcga cgcagctgcg ccttcgcact
    16081 cctgtcgggg aacggctcga cgacggcgga gagcccggcc tcgcgcagcc tcttcgcccc
    16141 ctcgagggcg gcgggcaccc cctcctcgcc gagaatcacg aagaccgcgt ccggcggcct
    16201 ctccccctcc gcccgatcct ccgggaggct catcaccagg cgatcctcgc cgacggcgaa
    16261 gccgaacccc ggcaccggcg gcccgccgag ctcctgcacg aggccgtcgt accgccctcc
    16321 gcccagaagc gcactctgcg ccccgagcgt cggcagcgtg acctcgaagg tcgtccggcg
    16381 gtagtagtcg agcccacgca ccaggcgcgg cgccatcgtg tacggcacgc cgaggcgctc
    16441 gagatggccg cgcactcctt cgagatgccg gcggcagtcg tcacacaggt gatcgaggat
    16501 cgggctcacc tgggcgatga gcgcctgatc ctccggcact ttgcagtcga agacgcgcag
    16561 gggattcgtc tccaggcgcc gctggcagtc ggcgtcgagc cggtcgcgga acggtctcag
    16621 ctccgctttg aggatttcgc ggtaggcggg acggcagttc tcgcagccca cggagttcaa
    16681 cagcagctcg atgcgggaga gcccgagccg atcgaggaac accgagagca tgtggagcgt
    16741 ctcggcgtcc accgccgggt gctcgctgcc gagggcctcg acgccgatct gcgcgaactg
    16801 ccgcatccgc cccttctgcg ggcgctcgta gcggaacatc gggccgatgt aatagagccg
    16861 cgtcaggtcg gagccgcgct gcatcccgtg ctgcagatac gcgcgggcca ccggcgcggt
    16921 gccctcggga cgcagcgtca gcgagcgtcc cttgcgatcg aggaacgtgt acatctcctt
    16981 cgcgacgatg tcggtcccct ccccgacgcc ccgcgcgaac agctcggtcg gctcgaagat
    17041 cggcgtgcgg atttcacgga agccgtagcg ctcgaacacc tcgcgtgcct gcgcctcgac
    17101 gaactgccag cgccccgcct ccgcgggcag gatgtcgcgg gtccccttga cggcttcgat
    17161 catggtgcgt gggtcctgat ccgtatccag aagactcggg ccggaagcgc cggagaatag
    17221 cacagggcac gcggctcccg aagcgggtgc ccgggctcct cccccgtata atgacggtcc
    17281 gatgcgcctc tcgcgcggcc gatcgcgccg cctcgtcgcc gccgtcgtca cgatcctgat
    17341 cctcccggtc gtcccgccgg tggacgcgcg ggacgccaag tcggtcttca gcggcctgcc
    17401 gatcgatccg gacatggcca agccggccga gcccgagcat ccgatcctgt tcgagccggc
    17461 ctggcggttc gacgggttct cggcgccgct cgccggcgac ctcgcggtct ccgagacgct
    17521 cctcgcaggc gtggacgtgg cgggacgcct gatcgccctc gaccccaagg aaggccggga
    17581 gcggtggcgc gtcgatctcg aggcggccgc cgcggtgggg ccggcgctct ccgatgagcg
    17641 ggtgctgcag ggaacgtccg acggacggct gcaggcgttc gccgcctccg acggacggcc
    17701 gctctggacc gccgatctgg gggcggcgcc ggcggtcgcg gcggtcgcca tcgccgggac
    17761 gatcctgcag gcgacgcggg ctcccgaggt cctggccatc aacccggagg acgggacggt
    17821 caccgcgcgg ctggcgctcg aatcgatccc gctgcccccg gtcgaagccc ggctgaagga
    17881 tggcgccgtc gaggcggtga tcgcggcgga ggaggggcag atccgactcg tcgagcccgg
    17941 gagcctcacg gtccgctggc gccgcgctct cggcca
//
LOCUS       DASRIQ010000473         6969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3301284, whole genome shotgun sequence.
ACCESSION   DASRIQ010000473 DASRIQ010000000
VERSION     DASRIQ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3301284"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VFD06_11585"
     CDS             <1..699
                     /locus_tag="VFD06_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HZN04213.1"
                     /translation="PDQVEPPGRVVTPAQRGRSADPHEASQDRATEPESAAADSGSDP
                     GDHAAMGSIGWAVVPQPDGESPLSPEPQDGPPPPKPVSPSPGPGDPTPIPEAIPTLQL
                     VPFRPALSVGESLVVAVRLSGASNVTSLPFHVEFDPAVLEFQAVEEGPAVGAKQPILL
                     ASVSPARPGDLAVGLSLVETGGLLQGSGTVMRLKFRAIGLGSSPLDFSRATIRGRISE
                     PLDARFQNASVQVH"
     gene            766..1977
                     /locus_tag="VFD06_11590"
     CDS             766..1977
                     /locus_tag="VFD06_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04214.1"
                     /translation="MRRPTVPILILAAAVGTLYAAFGVFERHRAGAVDPGWLDPDQIG
                     DIATHLCRSGEFAEREGAPSLLRGPAYPGLLAAPCLLTGIDPARLIPFVNGLCHALTL
                     LVLAFHPVARAAPGRLAALAAVGLDPLLLNYAGRAYLEPLLLLSVALVVVSVEAVRRR
                     PSPATGVLCGLALGLSLLVKPVLIYFAPLLLLLLLPSGRRAVVAGGVAIVTAALCVLP
                     WTLRNRAVSGRIVPVATGAWEIVLKGETFSRHVTEADGIVALEALAKERLARLDASLG
                     IAGLPAVEREPYYRAVALEEMRNPPWSLPRRMGIQSVSLWLLGGDRRNTLVFAALQLP
                     VLILTGVAVWRGRTFRPNDLVALAAVAGYLILVHGASLAIARYSMPLRPWLVLLAALA
                     FIPRLRRDVRV"
     gene            1970..2905
                     /locus_tag="VFD06_11595"
     CDS             1970..2905
                     /locus_tag="VFD06_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN04215.1"
                     /translation="MSDPGPYGGVAVLIPCYNEEITVRKVVEDFRRALPGSAIYVFDN
                     NSKDRTAAIAREAGALVVPSPRQGKGHVVRHMFQSVEAEAYLMVDGDATYPADQAERL
                     LEAFRAGGVDMVVGARIESSEAGAFRRFHRFGNHLVARLIASLFRVPATDVMSGYRVF
                     SRDFVKSIPLLSAGFEIETEMTLQAAAKGYVFREVPIDYGKRPEGSLSKLNTYSDGFL
                     VLRTILLIFKDYKPLVFFTLLAGLFALLSIASGWGPVEDYMTTGLVPRFPRAILAAAL
                     GILAGVSLGVGFILDTLAKFQREQFELWRRHFTRP"
     gene            complement(2909..3058)
                     /locus_tag="VFD06_11600"
     CDS             complement(2909..3058)
                     /locus_tag="VFD06_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HZN04216.1"
                     /translation="MDFLKELWAFMRERKKFWLLPIVLVLMLLGALIVLTQGSAIAPF
                     IYTLF"
     gene            complement(3074..3469)
                     /locus_tag="VFD06_11605"
     CDS             complement(3074..3469)
                     /locus_tag="VFD06_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HZN04217.1"
                     /translation="MEETVPSKTLRSFGLLVGGVWGLIGLWPLVWRREPPRLWALGLM
                     AVLVGLGLVFPQALRQPYRGWMALGHALGWINTRILLGLVFYLLVTPMGFVMRLFGRD
                     PMRRGFDASAKTYRIGREARPGSHMRHQF"
     gene            complement(3484..5340)
                     /locus_tag="VFD06_11610"
     CDS             complement(3484..5340)
                     /locus_tag="VFD06_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HZN04218.1"
                     /translation="MSEHILGISAYYHDSAACLLRDGEIVAAAQEERFTRKKHDARFP
                     KNAVRYCLREGGVALQDLRYVAFYDKPLVKYERLLETYLGFSPKGLRSFVAAMPIWLK
                     EKLFLKTLLGKEIADCADGEGNGARALPPILFGEHHESHAASAFYVSPFRRAAVLCLD
                     GVGEWATTSAWLGEEHRLTPLWEIHFPHSIGLLYSAFTYFTGFKVNSGEYKVMGLAPY
                     GRPTYVKQIYDHLMDLKPDGTFRLNMDYFAYCTGLRMTNGRFDDLFGGPPRQPESPLT
                     QREMDLARSVQEVTEEVMLRLSRTLHAETGAENLCLAGGVALNCVGNGRLLREGPYTK
                     IWIQPAAGDAGGAIGAALSAWHALDGQPRRLNGGLDAMRGAYLGPAYGNEEIESTLKG
                     LGAPYVRLDEDALFERVADELAAGKVVGWLQGRMEFGPRALGGRSILGDARNTKMQSV
                     MNLKIKYRESFRPFAPSVLRERVADFFEMDADSPYMLLVAPVKEGRRIPMTGEHDSLW
                     GIDLLNVPRSDIPAITHVDYSARVQTVHADTNPRYHRLLRAFEEKTGYGVLVNTSFNV
                     RGEPIVCTPLDAWRCFMRTEMDVLVLENCVLVKEGQPALKESGDWRKEFALD"
     gene            complement(5337..6554)
                     /locus_tag="VFD06_11615"
     CDS             complement(5337..6554)
                     /locus_tag="VFD06_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN04219.1"
                     /translation="MTRNESRPPAPFRRGVAAAAVLLGLLGGFLIAEAAVRLTLRYNS
                     PATVRAHSLQYLPAVYARHRLKPGQDFDAGLAWGPPPPGTPSGPRIAVNERGLRGTPV
                     AVPKPAGTVRIVVLGGSSVFDIHASEGEDWPHLAGARLREGGHPEAEVLNTGIPGHAS
                     ADSLGRLATQIWMLEPDYVLLVHGWNDLKLLGRIDPDHPLLARQAPYDPRADPFQNDR
                     GPLDRLLQSSQLYVKLRTRYFQSRVAPGTEGALGEDRGAAGLDWAWGERQFRIDLALL
                     AETCRAIGARPVFVTEATLVTADADEEARARIQYDYVGFDHPTLVRAYALFNDAVRAV
                     GRERGVPILDLDRALSGRKQLFADHVHTNAEGSRALADAAAAWLERALEAGSAAAPVA
                     LPGAPVNDRITRP"
     gene            complement(6551..>6969)
                     /locus_tag="VFD06_11620"
     CDS             complement(6551..>6969)
                     /locus_tag="VFD06_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04220.1"
                     /translation="IAVLLNDGGGLPLTAGIADFALRPGASPGALAITWRTVAERDVE
                     GFRLVEVGRLGTPAAETLVDCAECASGLGSSYRLLLARPAQGRTFYLDVLHSDGTSER
                     FGPAIKTAPGRDAVGQGKARRVKSRPGQSDPAPSGP"
BASE COUNT          977 a         2480 c         2433 g         1079 t
ORIGIN      
        1 cccgatcagg tcgagccgcc gggccgcgtt gtcactccgg cgcagagggg acggtcggcc
       61 gatcctcacg aggcgtcgca ggatcgcgcc acagagcccg agagcgcggc ggcggactcc
      121 gggagcgatc ccggcgacca cgccgcgatg ggatcgatcg gctgggcggt ggtgccgcag
      181 ccggacggcg agtcaccgct gtctcccgaa ccgcaggatg gcccgccgcc cccgaagccg
      241 gtgtcaccct cgcccggacc gggggatccg actcccatac cggaggcgat cccgacgctt
      301 cagctcgtcc ccttccggcc cgcgctctcg gtcggcgagt cgctcgtcgt ggcggtgcgc
      361 ctgagcggcg cctcgaacgt cacgagcctt ccgtttcacg tcgaattcga ccctgcggtg
      421 ctggaattcc aggccgtcga ggaaggcccc gccgtcggcg cgaagcagcc gatcctgctc
      481 gcctcggtca gcccggcgcg cccgggggat ctggcggtcg ggttgtcgct cgtcgagacc
      541 ggcggcctcc tgcagggctc cgggaccgtc atgcgcctga agttccgcgc catcggtctg
      601 ggatcttcgc cgctcgactt cagccgcgcc accatccgcg gccgcatcag cgagccgctg
      661 gacgcgcgct tccagaacgc ctccgtccag gtgcactgat tcgccggcgt cgcatcgtcg
      721 cgctggttgc gaattcccgg acaggcacct agaatctgcc gcgcgatgcg gcgaccgacc
      781 gtcccgatcc tgatcctggc cgcggcggtc ggcactctgt acgccgcgtt cggcgtcttc
      841 gagcgtcatc gcgccggcgc cgtcgatccc ggctggctcg acccggacca gatcggcgac
      901 atcgccacgc acctctgccg gtccggcgag ttcgccgagc gcgagggggc gccgagcctg
      961 ctgcgcggcc cggcgtatcc gggtctcctc gccgcgccgt gcctcctgac cggcatcgat
     1021 ccggcccggc tcatcccgtt cgtcaacggg ctctgccatg cgctgaccct gctcgtgctc
     1081 gcgttccatc cggtggcgcg cgccgcgccg gggcggctgg cggcgctcgc cgccgtgggg
     1141 ctcgatccgc tcctgctcaa ctacgcgggc cgcgcctacc tcgagccgct gctgctgctt
     1201 tccgtagccc tcgtcgtggt gtcggtcgag gccgtgcggc gccgcccctc ccccgcgacg
     1261 ggagtcctgt gcggcctggc gctcgggctg agcctcctgg tgaagccggt cctcatctat
     1321 ttcgcgccgc tgctcctgtt gctgcttctc ccgtccggcc ggcgcgccgt cgtcgcgggc
     1381 ggggtcgcca tcgtcacggc ggcgctgtgc gtcctgccct ggacgctgcg caaccgggcg
     1441 gtgagcggac ggatcgtccc ggtcgcgacc ggcgcctggg agatcgtcct caagggggag
     1501 accttttccc gccatgtgac cgaggcggac gggatcgtgg cgctcgaggc gctggccaag
     1561 gagcggctcg cccggctcga cgcgtcgctc gggatcgccg ggctccccgc cgtcgagcgg
     1621 gagccgtatt accgcgcggt ggcgctcgaa gagatgcgca acccgccctg gtccctgccg
     1681 cgccgcatgg ggatccagtc ggtctcgctg tggctgctcg gcggggaccg gcgcaacacc
     1741 ctggtgttcg cggcgctgca gctgccggtc ctgatcctga cgggggtggc ggtgtggcgc
     1801 ggccggacct tccgtcccaa cgacctcgtc gccctcgctg ccgtggccgg ctacctcatc
     1861 ctcgtccacg gcgcgagtct ggcgatcgcg cgctacagca tgccgctgcg cccgtggctc
     1921 gtcctgctgg cggcgctggc cttcatcccg cgcctgagga gggacgttcg tgtctgaccc
     1981 gggaccctat ggtggcgtgg cggttctgat cccctgttac aacgaggaga tcaccgtccg
     2041 caaggtggtc gaggacttcc gccgcgccct gccgggctcg gcgatctacg tcttcgacaa
     2101 caactcgaag gatcgcacgg cggcgatcgc ccgcgaggcg ggggcgctcg tcgtgccttc
     2161 gcctcgacag gggaaggggc acgtcgtccg ccacatgttc cagagcgtcg aggccgaggc
     2221 gtacctgatg gtcgacggcg acgccaccta cccggccgac caggcggagc ggctgctcga
     2281 ggcgtttcgg gcgggagggg tcgacatggt cgtgggcgcg cgcatcgaga gctcggaggc
     2341 gggtgccttc cggcgcttcc accgcttcgg gaaccacctg gtggcccgcc tgatcgcgtc
     2401 gctgttccgg gtgccggcga ccgacgtgat gtccggctac cgggtcttct cgcgcgactt
     2461 cgtgaaatcg atcccgctcc tgtcggcggg gttcgagatc gagaccgaga tgacgctgca
     2521 ggcggcggcg aaggggtacg tgttccgcga ggtccccatc gactacggca agcgccccga
     2581 ggggagcctc tcgaagctca acacctattc ggacggcttc ctggtgctgc ggaccatcct
     2641 gctgattttc aaggactaca agccgctggt gttcttcacc ctgctggcag ggctgttcgc
     2701 gctgctctcc atcgcgtccg gctgggggcc ggtcgaggac tacatgacga ccgggctcgt
     2761 gccgcggttc ccgcgcgcca tcctggccgc cgcgctcggg atcctcgccg gcgtcagcct
     2821 cggggtgggg ttcatcctcg acaccctcgc caagttccag cgcgagcagt tcgagctgtg
     2881 gcgccggcac ttcacccggc cgtaggtcct agaacagcgt gtagatgaac ggggcgatgg
     2941 ccgatccctg ggtcagcacg atgagcgcgc ccaggagcat cagcacgagg acgatcggca
     3001 gcagccagaa tttcttccgc tcccgcatga aggcccagag ttccttgagg aagtcgatca
     3061 actcgcgtct ccttcagaac tggtgccgca tgtgcgagcc cgggcgcgcc tcgcggccga
     3121 tccgataagt cttcgcgctc gcgtcgaacc cgcgccgcat gggatcccgg ccgaagagcc
     3181 gcatgacgaa ccccatcggc gtcacgagca ggtagaacac caggcccaac aagatccgcg
     3241 tgttgatcca gccgagcgcg tgcccgagcg ccatccagcc gcgataaggc tggcggagcg
     3301 cctgagggaa caccagaccg agcccgacga gcacggccat gagaccgagc gcccacaggc
     3361 gcggcggctc gcggcgccac accaggggcc acagaccgat gagtccccag acccctccca
     3421 ccaggagtcc gaaggaacgc agcgtcttgc tcgggacggt ctcctccatg gcgcaggtct
     3481 ccctcagtcg agggcgaact ccttgcgcca gtccccgctt tccttcaggg ccggctgtcc
     3541 ttccttcacg aggacgcagt tctcgaggac gagcacgtcc atctcggtgc gcatgaagca
     3601 gcgccaggcg tccagcggcg tgcagacgat cggctcgccc cgcacgttga acgaggtgtt
     3661 gacgagcacc ccgtagccgg tcttctcctc gaaggcgcgc agcaggcggt gatagcgcgg
     3721 attggtgtcc gcatgcaccg tctggacacg ggccgaatag tcgacgtggg tgatcgccgg
     3781 gatgtcggag cgcggcacgt tgaggagatc gatgccccag agcgagtcgt gttctcccgt
     3841 catcgggatg cgacgtccct ccttcaccgg cgccaccagc agcatgtagg ggctgtcggc
     3901 gtccatctcg aagaaatcgg ccacccgctc gcgcagcacc gacggggcga acggccggaa
     3961 cgactcgcga tacttgatct tgaggttcat gaccgactgc atcttcgtgt tgcgcgcgtc
     4021 cccgaggatg ctgcgtccac cgagggcgcg cggcccgaac tccatccgcc cttgcagcca
     4081 gccgaccacc ttccccgccg ccagctcgtc ggcgacccgc tcgaagaggg cgtcctcgtc
     4141 gagacggacg taaggggcgc cgagcccctt gagcgtcgat tcgatctcct cgttgccgta
     4201 cgccgggccg aggtaggcgc cgcgcatcgc gtcgagaccg ccgttcagcc ggcgcggctg
     4261 tccgtcgagg gcgtgccagg ccgacagcgc ggcgccgatc gccccgccgg cgtcccccgc
     4321 ggccggctgg atccagatct tcgtgtacgg cccctcccgc agcagccgtc cgttgccgac
     4381 gcagttgagg gcgacgccgc cggcgaggca gaggttctcg gcgccggttt cggcgtggag
     4441 cgtgcgcgac agccggagca tcacctcctc agtcacctcc tgcaccgagc gggcgagatc
     4501 catctcccgc tgcgtcaggg gggactccgg ctggcgcggc ggcccgccga acaggtcgtc
     4561 gaacctgccg ttggtcatgc gcaggccggt gcaataggcg aagtagtcca tgttgagccg
     4621 gaaggtgccg tccggcttga gatccatcag gtggtcgtag atctgcttca cgtacgtcgg
     4681 gcggccgtac ggggcgagcc ccatcacctt gtattcgccc gagttcacct tgaaccctgt
     4741 gaaataggtg aaggcggagt acagcaggcc gatcgagtgc gggaagtgga tctcccagag
     4801 cggcgtcagc cggtgctcct cgccgagcca ggcggaggtg gtcgcccact cccccacccc
     4861 gtcgaggcac agcaccgcgg cgcggcggaa cggagagacg tagaaggccg aggcggcgtg
     4921 ggactcgtgg tgctcgccga acaggatcgg cggaagcgcc ctcgcaccgt tcccctctcc
     4981 gtccgcgcag tcggcgatct ccttccccag aagcgtcttg aggaagagct tttccttcag
     5041 ccagatcggc atggcggcca cgaacgagcg cagccccttc ggcgagaagc caaggtaggt
     5101 ctcgagcagg cgctcgtact tcaccagcgg cttgtcgtag aaggcgacgt agcgcaggtc
     5161 ctgaagcgcc acccctcctt cgcgcaggca gtagcgcacc gcgttcttcg gaaagcgcgc
     5221 gtcgtgcttc ttgcgggtga aacgctcttc ctgcgccgcg gcgacgatct ccccgtcccg
     5281 caggagacac gcggcgctgt cgtgatagta ggccgagatt ccgaggatgt gctcgctcat
     5341 ggacgcgtga tcctatcgtt gaccggcgcc ccgggtaaag cgacgggagc ggccgcagag
     5401 ccggcttcga gggcacgctc gagccacgcg gccgcggcgt cggcgagggc gcggctccct
     5461 tcggcgttgg tgtggacgtg gtcggcgaac agctgcttgc ggccgctcag ggcgcgatcg
     5521 agatcgagga tcggaacgcc gcgctcgcgg ccgaccgcac gcacggcgtc gttgaacaag
     5581 gcgtaggcgc gcacgagcgt ggggtgatcg aagccgacgt agtcgtattg gatgcgcgcg
     5641 cgggcctcct cgtcggcgtc ggcggtgaca agcgtggcct cggtgacgaa caccggccgc
     5701 gccccgatcg cccggcaggt ttccgccagg agcgcgaggt cgatccggaa ctgccgttct
     5761 ccccacgccc agtcgagtcc cgcggcgccc cggtcctccc cgagcgcccc ctccgtcccc
     5821 ggcgcgacgc gcgactggaa gtaccgcgtc ctcagcttga cgtacagctg cgacgactgg
     5881 aggaggcgat cgagggggcc gcgatcgttc tggaacgggt cggcccgcgg atcgtagggc
     5941 gcctgacgcg ccaggagcgg gtgatcggga tcgatccgcc cgaggagctt caggtcgttc
     6001 cagccatgca ccagcagcac gtagtccggc tccagcatcc agatctgcgt ggcgagccgt
     6061 ccgagcgagt cggcgctggc gtgtcccggg atgccggtgt tgagcacctc cgcctcggga
     6121 tgccccccct cgcggagccg ggcaccggcg aggtgcggcc agtcctcccc ttcactcgca
     6181 tggatgtcga agaccgacga acctccgagc acgacgatcc gcaccgtccc cgccggcttc
     6241 ggcaccgcga ccggagtgcc gcgcaggccg cgctcgttga ccgcgatgcg cggtccggag
     6301 ggggtcccgg gtggaggcgg gccccacgcc aggccggcat cgaagtcctg ccccggcttg
     6361 aggcggtgac gcgcgtaaac ggccggcagg tactgcagcg agtgcgcccg gaccgtcgcc
     6421 ggggagttgt agcgcagggt cagccgcacc gccgcctcgg cgatgaggaa tccgcccagg
     6481 aggccgagga ggacggccgc ggcggcgacg cccctgcgga agggggccgg cggtcgggac
     6541 tcatttcggg tcacggtccc gagggggcgg ggtcgctctg acccggccgc gacttcacgc
     6601 gccgagcctt cccctgcccc accgcgtcgc gtccgggggc ggtcttgatc gccgggccga
     6661 accgctcgct cgtgccgtcc gagtggagca cgtccaggta gaacgtccgt ccctgggcgg
     6721 gccgcgccag cagcagccga taggaggagc cgaggccgga ggcgcactcc gcgcagtcca
     6781 cgagcgtctc cgctgccggg gtccccaggc ggcccacctc caccagtcgg aaaccctcga
     6841 cgtcccgctc cgccacggtg cgccaggtga tggccagggc cccaggcgag gctcccggcc
     6901 gcagcgcgaa gtcggcgatc ccggcggtga gggggaggcc accgccgtcg ttgagcagga
     6961 cggcgatcg
//
LOCUS       DASRIQ010000474         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2267177, whole genome shotgun sequence.
ACCESSION   DASRIQ010000474 DASRIQ010000000
VERSION     DASRIQ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267177"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VFD06_11625"
     CDS             complement(<1..966)
                     /locus_tag="VFD06_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04221.1"
                     /translation="MAALLVSVLQSQGLAEAADVNIRPASACVIPPRVAGPDGIPGTL
                     DDGILAEDFDTERDGSPGISLAAQPRGTPGVLNDTIGVWVGTAAGANVLGAVGCAGFR
                     VPPQDPACVIDPDHDMDWHIHCPPGTCPNQAGHVTPLDGALAASGSNSLHWGHHFDPD
                     SRDGDSTKVRQLAAFMTDPIHLTPAPHSGDLELSFFHIADMMDNSSHGFPVGQANDFG
                     DVQIQVLDPHAPSGGQWGYWDKLVPFQNVYDHISYIWSTYGGPTYCVLTPTDTGPGGY
                     APRGSRETLCFPNGVWSRCGNSRHSFDTGHCIGPGRPGATGPGLWV"
     gene            complement(1074..>3077)
                     /locus_tag="VFD06_11630"
     CDS             complement(1074..>3077)
                     /locus_tag="VFD06_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZN04222.1"
                     /translation="LGQLLPAPEGRAEYERSLSISRRDDDVEGIGAALRGLAEYIAVE
                     EPDRLAEAHRALDEALAIARRSGNLEEMVRASLSRATIEWRRGPRDRAVADGLATLDG
                     IDALRDLQPEEAARSGIVSRWLPAYRGLAGFLLASDSGVPGPDGLALAFAVQERMRAR
                     VLREALDAALRAEDGAQPRPPSPADERIAATRAQIAGVQRLLIDPALSGAARQAALSR
                     LEQLEIDEAALRRDASGGLAAGAAAPGPTRAAAIEEVRRALPADTALLAYQVAPRAHL
                     RDLPGGAWLVVVTAGRARAYPLPEPSSLASTIGMFTGLIERRDGREGPAAARLYHDLL
                     EPALGDLPSTVTHLVFVLDGILHAVPFAVLRPAPDADPLAARYDLTAAPSATLWLRFR
                     EAAARPATIPAIAYADPDLGAPVNGRNEAVATVGASRAWAFGLGARLGAMPRARAEGR
                     SLVDRLGGGSRLLAGKEASERSVKSGDLGRFAIVHFAAHAVLDDEHPERSSLVLAPGD
                     PGEDGLLQPREVVDLELGGRLVVLSSCRSASGAILAGEGVMGLARAFFRAGARTVVGS
                     LWSLRDADAADLFEQFYERIGRGESVAGALAGAQRDLRSRGRPAAAWAGLVVLGDGSA
                     VPVPAGTAHPAGPAWLSWAAAALAGAIIVATALLLVRRARAGG"
BASE COUNT          430 a         1121 c         1153 g          373 t
ORIGIN      
        1 cacccagaga ccggggccgg tcgcgcccgg ccggcccggg cctatgcaat gaccggtatc
       61 gaatgagtgc cgcgagtttc cgcagcgcga ccagacgccg ttcgggaagc acaaggtctc
      121 ccggctccct cgaggtgcgt aaccccccgg gccggtatcg gtgggcgtca acacacagta
      181 ggtcggccct ccataagtgg accagatgta ggagatgtgg tcgtacacgt tctggaacgg
      241 gaccagcttg tcccagtagc cccactggcc gccgctcggc gcgtgcgggt cgagcacctg
      301 gatctgcacg tcgccgaagt cgttcgcctg tcccacggga aaaccatggg aactgttgtc
      361 catcatgtcg gcgatgtgga agaacgacag ctccagatcg cccgagtgcg gggccggcgt
      421 caggtggatc gggtcggtca tgaaggcggc cagctggcga accttcgtgc tgtccccgtc
      481 gcggctgtcg ggatcgaagt ggtgccccca gtgcagcgag ttcgagccgc tcgcggccag
      541 cgccccgtcg aggggcgtca cgtggccggc ctggttcgga caggtcccgg gcgggcagtg
      601 gatatgccag tccatgtcgt ggtcggggtc gatgacgcag gccgggtcct gtggcgggac
      661 ccggaatccg gcgcagccca cagcgccgag gacgttcgcc cccgccgcgg ttccgaccca
      721 gacgccgatc gtgtcgttca gcacccccgg ggtgccccgc ggctgcgcgg cgaggctgat
      781 gcccggcgag ccgtcgcgct cagtgtcgaa gtcctcggcc aggatcccat cgtccagcgt
      841 gccggggatt ccatccggcc ccgcgacccg gggaggaatc acgcacgccg aggccgggcg
      901 gatgttgaca tcggccgcct cggccaggcc ctgtgattgc aggacgctga cgagcaatgc
      961 ggccatcgat ggcagggtcc ggcgcatggc gccccccgga tttcgaacga acgacggtga
     1021 gccgagtata agcgctcacc ggtcgtcctg gcgcagcatt cacctcatcg ggctcagccg
     1081 cccgcccgcg cacgacgcac gagcaggagg gcggtcgcga cgatgatggc gccggcgagc
     1141 gccgccgcgg cccaggaaag ccacgccgga cccgctgggt gcgccgtgcc ggcggggacc
     1201 gggaccgcgc tcccgtcgcc gaggacgacg aggcccgccc acgccgccgc cgggcggccg
     1261 cgggatcgca ggtcacgctg cgccccggcg agggcgccgg cgacgctttc gccgcggccg
     1321 atccgctcgt agaactgctc gaagagatcg gcggcgtcgg cgtcgcgaag cgaccagagg
     1381 ctgccgacga cggtacgcgc gccggcccgg aagaatgccc gcgccaaccc catcaccccc
     1441 tcccctgcga ggatcgcgcc ggaggcgctg cggcaggagg agagcaccac cagccggccg
     1501 ccgagttcga gatcgacgac ctcgcgcggc tgcagcaggc cgtcctcgcc ggggtcgccg
     1561 ggggccagca ccagcgacga acgctccggg tgctcgtcgt cgaggacggc gtgcgcggcg
     1621 aagtggacga tggcgaatcg ccccaggtcg ccgctcttga ccgagcgctc gctcgcctct
     1681 tttccggcaa ggaggcggct gccgccgccg agccgatcga cgaggctccg cccttcggcg
     1741 cgcgcgcgcg gcatggcgcc cagtcgtgcc ccgagcccga acgcccaggc gcgcgacgcc
     1801 ccgacggtgg ccacggcctc gttccggcca ttcaccgggg cgccgaggtc gggatcggcg
     1861 taggcgatgg ccgggatcgt cgccggccgg gcggcggctt cgcggaaacg cagccagaga
     1921 gtggcggacg gcgccgcggt caggtcgtag cgcgcggcca gcggatcggc gtccggcgcc
     1981 ggacgcagca cggcgaaagg cacggcgtgc aggatcccgt ccaggacgaa cacaaggtgc
     2041 gtgacggtgg acggcaggtc gccgagcgcc ggctcgagga gatcgtggta caggcgcgcc
     2101 gccgcgggcc cctctcttcc gtcgcgccgc tcgatcaggc cggtgaacat cccgatggtc
     2161 gaggcgaggg aggacggttc gggaagcgga taggcccgcg ctcggccggc cgtcacgacg
     2221 acgagccacg cgccgcccgg aagatcgcgc aggtgagcgc gcggcgcgac ctgataggcg
     2281 aggagcgccg tgtccgctgg cagggcgcgg cgcacctcct cgatcgcggc ggcgcgcgtc
     2341 ggcccgggtg ccgcggcgcc ggccgccagc cccccggacg catctcgacg cagcgccgcc
     2401 tcgtcgatct cgagctgctc gagccgggac agcgccgcct gccgcgccgc gccggagagc
     2461 gccggatcga tcagcagccg ctgcacgccg gcgatctgcg cgcgcgtcgc ggcgatccgc
     2521 tcgtccgcgg ggctgggcgg ccggggctgc gcgccgtcct cggcgcgcag cgccgcgtcg
     2581 agtgcttcgc gcaggacccg cgcgcgcatc cgctcctgga cagcgaatgc cagcgccagg
     2641 ccatccggtc ccgggacgcc gctgtccgac gccaggagga acccggccag tccgcggtag
     2701 gcgggcagcc agcgcgacac gatcccggag cgcgccgcct cctccggctg caggtcgcgc
     2761 agggcgtcga tgccgtcgag ggtggcgagg ccgtcggcca cggcccggtc acgcgggccg
     2821 cggcgccact cgatcgtcgc gcgcgacagc gacgcccgca ccatctcctc gaggttgccg
     2881 gagcggcggg cgatcgccag cgcctcgtcc agcgcccggt gcgcctcggc gaggcgatcc
     2941 ggctcctcca ccgcgatgta ctcggccaga ccgcgcagcg cggcgccgat tccttcgacg
     3001 tcgtcgtccc ggcgcgagat cgagagcgag cgctcgtatt cggcgcggcc ttcgggggcg
     3061 gggaggagct gcccgag
//
LOCUS       DASRIQ010000475         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2808704, whole genome shotgun sequence.
ACCESSION   DASRIQ010000475 DASRIQ010000000
VERSION     DASRIQ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2808704"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..>2165
                     /locus_tag="VFD06_11635"
     CDS             51..>2165
                     /locus_tag="VFD06_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04223.1"
                     /translation="MIPLRLSLAFFASGAAALIYEALWLRRFALLLGATAPATAAVLS
                     ALFLGVALGSALFGMPSVRARRPLLLFAAIETGIAAAAFAVEPLLAWIGDPLAAAGNA
                     HPDFGLVLRMGTAMATVLPATILMGGSFPVLAQALHRGGGGLGRLGGGLYAVNTLGAA
                     LGVLAVPFALLPSCGAAATLWIAAALNVLAALAACSLSRAPRAAPTPSIGPSARLASG
                     GWLIAGAFLSGCVALSLETLWTRMLALVHENSLQAFAVVTAIFLAGLAAAAAGARRLI
                     ARGVSARRVAAVGWMLAGGWIVATPPIFHALTSGLRALDAGRGMALHLAQIGGLACAT
                     ILPATLFAGAALPALMETAGAGRVAAAGPVLGGLLFVNTMGAFAGPLLASFVVGPLFG
                     LWGGLVLCGLLLLVAGQATLPGVARRGPLLRRLAISSAAAGLLALWAPWSLPVVRLGP
                     GERLIDLAEGPFGAVGVVESNGDRRMTLNNSYVLGGTASTGEERLQAHLPLLLHPAPE
                     RVAFLGVGTGITTGASLFHPVREVAAIELVPDVLRAARRFFREANLGVLEDPRVRVYA
                     EDARTRLRASPGRFDVIVGDLVVPWRRGEAGLHTREGFETARRALAPGGVFCQWIPLF
                     QLSQSEFESIAAAFLDVFRESLLFRGDFRAGEPALALVGFTNAGPLDSARIDARSEAL
                     ARRADPANPYLAHPAGLWVYLAG"
BASE COUNT          206 a          809 c          783 g          367 t
ORIGIN      
        1 cgcgccaccg gcgggtcggc ggatgaacct cgcggtctac tatgcgcagg ttgatcccgc
       61 tccggctctc gctcgctttc ttcgcctcgg gagcggcggc cctcatctat gaggcgctgt
      121 ggctgcggcg cttcgcgctg ctcctggggg ccaccgctcc ggccaccgcc gcggtcctct
      181 ccgcgctgtt cctcggggtc gcgctgggca gcgctctctt cggcatgccg tcggtgcgcg
      241 cgcgccgccc gcttcttctg ttcgcggcga tcgagaccgg gatcgccgcc gccgcgttcg
      301 cggtcgagcc gctgctcgcc tggatcggcg acccgctcgc cgcggccggg aacgcgcatc
      361 cggatttcgg actcgtcctc aggatgggaa ccgcgatggc gacggttctg ccggcgacga
      421 tcctgatggg aggaagtttc ccggtgctgg cgcaggcgct ccatcgcgga ggcggcggcc
      481 tcggccggct cggcggcggc ctgtacgccg tgaacacgct cggagccgcc ctcggagtcc
      541 tcgccgtccc gttcgccctc ctcccttctt gcggcgccgc ggcgacgctg tggatcgcgg
      601 cggcgctcaa cgtcctggcc gccctggccg cctgcagcct gagtcgcgcg ccgcgcgcgg
      661 caccgacacc gtcgatcggc ccatccgccc gcctggcctc gggtgggtgg ctcatcgcgg
      721 gcgccttcct gtccgggtgc gtggcgctct cgctcgagac gctctggacg cggatgctgg
      781 ccctcgtgca cgagaactcg cttcaggcct tcgctgtggt cacggccatt tttctggccg
      841 gtctcgcggc cgcggcggcc ggtgcgcgcc ggctcatcgc ccgcggcgtc tccgcgcgcc
      901 gcgtcgccgc ggtcgggtgg atgctggccg gaggatggat cgtcgcgacc ccgccgatct
      961 tccatgctct gacgtcggga ctgcgcgcgc tcgacgcagg acgcgggatg gcgctccatc
     1021 tggcccagat cggagggctg gcgtgcgcca cgatcctgcc ggccactctc ttcgccgggg
     1081 cggcccttcc ggccctcatg gagacggcgg gagcgggacg ggtcgccgcc gccggtccgg
     1141 tgctcggcgg cctcctgttc gtcaacacga tgggggcgtt cgcgggaccg ctcctcgcct
     1201 cgttcgtcgt cggacctctg ttcgggctgt ggggcgggct ggtcctgtgc ggtcttctcc
     1261 tgctcgtcgc cgggcaagcg acgcttcccg gagtggcccg gcgggggcca ctcctccggc
     1321 gactggcgat ctcctccgcc gcggccggcc tgctggcgct ctgggcgccg tggagcctcc
     1381 ccgtggtgcg actggggccc ggggaacgcc tgatcgatct cgccgaagga ccgttcggag
     1441 ccgtcggggt ggtcgagtcg aacggcgacc gacgaatgac gctcaacaac tcctacgtgc
     1501 tcggcgggac cgcctcgacg ggcgaggagc gcctgcaagc ccacctgcct cttctcctcc
     1561 accccgctcc ggagcgcgtc gcgttcctcg gcgtcggcac cgggatcacc accggggcgt
     1621 ccctgttcca tccggtccgc gaggtggcgg cgatcgagct ggtgccggac gtcctccggg
     1681 cggcgcgccg cttcttccgc gaggccaacc tgggcgtgct ggaggacccg cgcgtgcgcg
     1741 tctacgccga ggacgcgcgc acgcgactgc gggcgtcgcc gggacggttc gacgtcatcg
     1801 tcggcgacct ggtcgtcccc tggcggcgcg gagaggccgg gctgcacacc cgcgaggggt
     1861 tcgaaacagc gcgacgggcc ctggccccgg gcggcgtctt ctgccagtgg atccccctct
     1921 tccagctctc gcaatcggag ttcgagagca tcgccgccgc gtttctcgac gtcttccggg
     1981 aatcgctgct cttccgaggc gacttccggg cgggagaacc ggcgctcgcg ctcgtcgggt
     2041 tcacgaacgc ggggccgctc gactcggcgc ggatcgacgc ccgctcggag gcgctggcgc
     2101 ggcgcgcgga tccggccaac ccgtacctcg cgcatccggc gggcttgtgg gtctacctcg
     2161 ccggt
//
LOCUS       DASRIQ010000476         4325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1243605, whole genome shotgun sequence.
ACCESSION   DASRIQ010000476 DASRIQ010000000
VERSION     DASRIQ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243605"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFD06_11640"
     CDS             <1..477
                     /locus_tag="VFD06_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04224.1"
                     /translation="GGSLTSDQRFASGELTPTLEAVRGSQLERGFGMSDTLGARAAAE
                     RNAAANRRASAARASALAPGQDLDDYLSRLTGLANPYGTSTTNSSRSGAGGGGGGYAG
                     PSPTEAGIMGGLGAGLSAWGAFQGTGASRGGGGTATGAGNSDYANAAARQRQGPYW"
     gene            481..1419
                     /locus_tag="VFD06_11645"
     CDS             481..1419
                     /locus_tag="VFD06_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04225.1"
                     /translation="MGRFTQDPYSRRALQDALMAFGGRLSAGAGTEGYGALGPSVAVG
                     VGTFRESRDYGEKQRLIEEERRREAANDELRRRIEEARLRQADERIEQTDRRLSSTEE
                     RAEADRKAREARDAAAAEERRIGNERADKRLGLSEDAAGRSAASLAGVESRFSASEAR
                     RDRERREDAERNQRTEARIVANERHEAAQDEYRNGLRDEAPPSFESLFDEELVIRGLA
                     PMRQRPATGSPTPVTTAPAGAPKPPPETPRVPAKPLGGIGAAAKASPSTEEMPESVRA
                     LAGGNAIAVAKALKLARGGMPWDDIERLLREAIAGR"
     gene            1423..>4325
                     /locus_tag="VFD06_11650"
     CDS             1423..>4325
                     /locus_tag="VFD06_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04226.1"
                     /translation="MPANPFSGLFGAPAPSAARNPFSGLFGGAGPLDEEETLSERLER
                     ERLEAEEKTKAEQRRLREEREREMREMGATDAVAPARRANPLDALRRAGVAMTAAVPA
                     VSNIKESAARLLLPAGPIEDAAVRGLTGTERSLARRGEELTGLPLTNEGVARRTDEWR
                     SLEHPGQSPLEEALGDTVLEGVGGMLDVPGPGGAAGLLARRLGRGATAVPAGMLASAE
                     RRALPALRAEVDAIMPKLAGKTREELAGLAASAKPGTALSIAARRAMGRADLEDAGVG
                     VGEEFAARPGPRAAAAAPVTPEEAAAKAARFRAALAKKATTGPPPGMVAEEAAPFDVA
                     AGHPTRPTAQKLEPPDVPFEFNARRLNLSEADAGQLLSTIRENMPAIQAQRRAGQGGW
                     DKFSAPDRLAARLGITKERLLKTKAGTTFNDEEFAVLDSTLGGVSERISSISKEIATG
                     AATEAARAELAKLTVEYATLLKVSVGAGSEAGRALGSLRAFKKAMALPDRARMAVLKK
                     YGSLMDDAKVERLATIDNPDDMAAFLRTLDQPKAGELVHEAYISSLVSGLASQERNLL
                     GNSVMALTEQGARTLRRTAAAAGDVIRSRVTGAPRQHFASEATAGWVGMGHGFRRGLS
                     KAAVVFRKGYDPATPLDELVGKFQFGGAFARHPSKAVRALGLVITPPLRALSAVDAFY
                     KTIGFTSEQYALATKAALRSGKRGEELAGAVAEHLNDPDILEAAGKYAAGVTLTDAPS
                     EVTARFLRFRDAPAQSAPAKVARGGIKFIAPFVKIADRLMVKGFEYTPVGAIRGVSQM
                     AKGATTEGADLLARSAIGSTVMLGVAGMAEEGNLTAWAPRDDIEKERFYAAQKQPWSM
                     KIGNRWVPYAQLEPFSTPIAIVAAARQAYEESGELPPADVALHVMQALAQRTLDASYM
                     DSVQSLFESMSSDERAADKLARISSGIATGVAVPYSGAVRSVAQAQDPRYVRP"
BASE COUNT          654 a         1566 c         1594 g          511 t
ORIGIN      
        1 ggcggctcgc tcaccagcga ccagcgcttc gcctcgggcg agctcacccc gacgctcgag
       61 gcggtgcgcg gcagccagct cgagcgcggc ttcggcatgt ccgacaccct cggcgcccgc
      121 gccgcggccg agcgcaacgc ggcggccaac cgccgggcct ctgccgcccg ggcgtcggcg
      181 ctggcccccg gccaggacct cgacgactac ctctcccggc tcaccgggct cgccaacccc
      241 tacggcacct cgaccaccaa cagcagccgg tcgggcgccg gtggtggcgg cggcgggtac
      301 gctggcccct cgccgacgga ggccggcatc atgggcgggc tcggcgccgg gctctccgcc
      361 tggggcgcct tccagggcac cggggcgagc cggggcggcg gtggcacggc gaccggcgcc
      421 ggcaactccg actacgcgaa cgcggcggcg cgccagcgtc agggtccgta ctggtaggcg
      481 atgggacgct tcactcagga cccgtacagc cgtcgcgccc tgcaggacgc gctcatggcc
      541 tttggcggcc ggctctccgc tggcgccggt acggaggggt acggcgccct cggcccctcc
      601 gtcgcggtcg gcgtcggcac cttccgcgag tcccgcgact acggcgagaa gcagcggctc
      661 atcgaggagg agcggcgccg ggaggccgcc aacgacgagc tccgccgccg gatcgaggag
      721 gcgcgtctgc gccaggccga cgagcggatc gagcagaccg accgcaggct gtcgtcgacc
      781 gaggagcgcg cggaggccga ccgcaaggcg agggaggccc gcgacgcagc ggcagcagag
      841 gaacggcgca tcggcaacga gcgcgccgac aagcggctgg gcctctcgga ggacgctgcc
      901 gggcgatcgg cggcgtccct ggccggagtc gagagccgct tttccgcgag cgaggcgcgc
      961 cgcgatcgag aacgccgcga ggacgccgag agaaaccagc gcaccgaggc ccgcatcgtg
     1021 gcgaacgagc ggcacgaggc ggcacaggac gagtatcgca atggcctgcg cgatgaggcc
     1081 ccgccgagct tcgagtcgct cttcgacgag gagctggtga tccgcggcct cgcgccgatg
     1141 cgccagaggc cggctaccgg atctccgacg cccgtcacga ccgcgcctgc cggggcgccc
     1201 aagcctccgc cagaaacccc gcgtgtcccg gcgaagcctc ttggcgggat cggcgccgcg
     1261 gccaaggcgt cgccctccac ggaggagatg ccggagtccg tgcgggcgct cgccggtggc
     1321 aacgccattg cggtggcgaa ggcgctcaag ctggcccgcg gcggcatgcc ctgggacgac
     1381 atcgagcgtc tgctgcgcga ggccatcgcc ggtaggtagg ccatgccggc caatcccttc
     1441 tcggggctct tcggcgcacc ggcgccctcc gcggcgcgca acccgttctc cggcctcttt
     1501 ggcggagccg gcccgctcga cgaggaggag acgctgtccg agcgcctgga gcgcgagcgc
     1561 cttgaggccg aggagaagac caaggccgag cagcgccggc tccgcgagga gcgcgagcgc
     1621 gagatgcgcg agatgggggc aaccgacgcg gtggcgccgg ctcgacgcgc gaacccgctc
     1681 gacgcgctgc gccgcgccgg cgtcgcgatg acggccgcgg tgccggcggt gagcaacatc
     1741 aaagagtccg ccgcgcgcct cctgttgccg gcgggtccga tcgaagacgc cgcggtgcgc
     1801 ggactcaccg gcaccgagcg atccctggcg aggcggggcg aagagctgac gggcctaccg
     1861 ctcacgaacg agggagtggc gcgacgaacc gacgaatggc gctcgctgga gcaccccggg
     1921 cagtctccgc tcgaggaggc cctaggcgac accgttctgg agggagtcgg cggcatgctc
     1981 gacgtgccgg gccccggcgg cgcggcggga cttctcgctc gccgcctcgg tcgcggcgcc
     2041 accgccgtgc cggcgggaat gcttgcatcc gctgagcggc gcgcccttcc ggccctccga
     2101 gccgaggtcg acgccatcat gccgaagctg gccggcaaga cgcgggagga gctcgccggg
     2161 cttgccgcga gcgccaagcc aggaacggcg ctctcgatcg cggcgcgccg ggcgatgggc
     2221 cgcgccgact tggaagatgc cggagtgggc gtgggggagg agttcgccgc gcgcccaggg
     2281 ccgcgcgcgg cggccgcggc gcctgtcacc ccggaggagg ccgcggccaa ggcggcgcgc
     2341 ttccgtgctg cgctcgcgaa gaaggcgacc acgggccctc cgcccggcat ggtcgctgaa
     2401 gaggcggcgc cgttcgacgt cgcggccgga cacccgacac ggccaaccgc gcagaagctg
     2461 gagccgcccg acgtgccctt cgagttcaac gctcgccggc tgaatctgtc cgaggcggac
     2521 gccgggcagc tgctctccac gatccgcgag aacatgccgg cgatccaggc gcagcggcgc
     2581 gccgggcagg gcggatggga caagttctcc gcgcccgacc gtctcgcggc gcgactcggg
     2641 atcacgaaag agcggctcct caagacgaag gcggggacga ccttcaacga cgaggagttt
     2701 gccgtcctcg actcgacgct cggcggcgtc tccgagcgca tctcctcgat ttcgaaggag
     2761 atcgcgacgg gagccgccac cgaggcggcg cgggccgaac tggcgaagct caccgtcgaa
     2821 tacgcgacgc tgctcaaggt gtcggtgggc gccggatccg aagctggccg cgccctcggc
     2881 tcgctccgcg ccttcaagaa ggcgatggcg ctgcccgacc gggcgcgcat ggccgtgctc
     2941 aagaagtacg gctcgctgat ggacgacgcc aaggtcgagc gactcgccac gatcgacaac
     3001 ccggacgaca tggcggcctt cctgcggacg ctcgaccagc cgaaggcggg ggagctggtg
     3061 cacgaggcct acatctcctc tctcgtctcc ggcctcgcct cgcaggagag aaacctcctc
     3121 ggcaactccg tcatggccct gacggagcag ggcgcccgaa cgctgaggcg cacggcggct
     3181 gccgcaggcg acgtcatccg ttctcgggtc acgggtgcgc cccgccagca cttcgccagc
     3241 gaggcgaccg ccggctgggt cggcatgggc cacggcttcc gccgcggact cagcaaggcg
     3301 gcggtcgtct ttcgcaaggg ctacgacccg gcgacgccgc tcgacgagtt ggtggggaag
     3361 ttccagttcg gcggcgcctt cgcgcgccac cccagcaagg ccgtccgcgc cctcggcctg
     3421 gtgatcaccc cgccgctgcg ggctctctcc gccgtggacg ccttctacaa gacgatcggc
     3481 ttcacgagcg agcagtacgc gctcgccacg aaggcggcac ttcgcagcgg caagcgaggg
     3541 gaggagctgg ccggggcggt cgctgagcac ctgaacgacc cggacatcct ggaggctgcc
     3601 ggcaagtacg ccgccggcgt caccctgacc gacgcgccga gcgaggttac cgcccgcttc
     3661 ctgcgcttcc gcgacgcgcc ggcgcagagc gcgccggcga aggtggcccg cggcggcatc
     3721 aagttcatcg cccccttcgt gaagatcgcc gaccggctga tggtcaaggg ttttgaatac
     3781 acgccggtcg gcgccatccg cggcgtgagc cagatggcga agggcgccac gacagagggc
     3841 gcggatctgc tcgcccgctc ggcgatcggc tcgacggtaa tgctcggcgt cgccgggatg
     3901 gccgaggagg gaaacctcac tgcctgggcg ccgcgagacg acatcgaaaa ggagcggttc
     3961 tacgccgcgc agaaacagcc ctggtcgatg aagatcggca accgatgggt gccctacgcg
     4021 cagctcgagc cgttctccac gccgatcgcc atcgtggcgg ccgcccggca ggcctacgag
     4081 gagagcggcg agttgccgcc ggcggacgtc gcgcttcacg tcatgcaggc gctcgcccag
     4141 cgcaccctcg acgcgagcta tatggactcg gtgcagagcc tcttcgagtc gatgagcagc
     4201 gacgagcgcg cggccgacaa gctcgcgcgc atcagctccg gcatcgcaac cggcgtcgcc
     4261 gtcccctact ccggggccgt tcgcagcgtg gcgcaggcgc aggacccgcg ctacgtcaga
     4321 ccgga
//
LOCUS       DASRIQ010000477         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2267581, whole genome shotgun sequence.
ACCESSION   DASRIQ010000477 DASRIQ010000000
VERSION     DASRIQ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267581"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /gene="lysS"
                     /locus_tag="VFD06_11655"
     CDS             complement(<1..753)
                     /gene="lysS"
                     /locus_tag="VFD06_11655"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZN04227.1"
                     /translation="MTAHVRDQHWADEVAAQIPTQGGRHEISTGISPSGEIHIGNLRE
                     VITADVVARALRERGVEVVLDYVADNFDPLRKVYPFLDRSVYEPLIGAPLSEIPCPCG
                     KHPSYAAHYLEPFLDSLRRLRIDVKVIYADQMYKSGFLVPQIVQALEGRGAIARILQE
                     CTGRQVEDGWSPFNPICATCRRMTETQVRGYSAKDETVDYACRCGATGTVSMRGGGKL
                     TWRVDWPARWARIPVTVEPFGKDHATRGGSYDT"
     gene            complement(750..1370)
                     /locus_tag="VFD06_11660"
     CDS             complement(750..1370)
                     /locus_tag="VFD06_11660"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZN04228.1"
                     /translation="MKALGLFATLGCIAGLAAFAAEPPVQAPAPAAGVRTFKFDAAGS
                     KLGFTVTRPGEVVEGTATQFSGTVRIDPEHPDRGGSVALEVDAATMVTGNRLRDRTMR
                     NSHLETGTYPKVRFQSSSAGTKPDPLRPGEKRPVDLQGKLDLHGVTRTIKFPVTVGYD
                     GALLTADGTVSFTLSEFSIPIPKILWFVLDDKVTVTFHAVAKPESR"
     gene            complement(1367..1945)
                     /locus_tag="VFD06_11665"
     CDS             complement(1367..1945)
                     /locus_tag="VFD06_11665"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN04229.1"
                     /translation="MSLPGSLYLPFVAAAVVAAVLERRVALRNERRLLAEGGTEVAPA
                     VFGWMVPVYALHFAAAAWEHLALARSPAPSLVVAMLLLFAASKWLKWAAVRALGDAWT
                     MRVILPARPRVASGGPYRHLRHPNYVAVMGEILALPLAGGAYLTAAVFGAAFGMVLVA
                     RVRTEEAALMARPEYVAAMGSRPRFLPGRRSR"
     gene            complement(1959..3215)
                     /locus_tag="VFD06_11670"
     CDS             complement(1959..3215)
                     /locus_tag="VFD06_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN04230.1"
                     /translation="MTRDVVVVGGGPAGASTAAFLARLGRNVLVCEAARFPREKICGE
                     FLPPSVAPRLERLGALRDVEALAPRRPIGMAVVAPGGARVLGRFGAEAGPGYALRRPD
                     LDAVLLRAARRAGAEVLEGTRVTDVTPHPGGGYVLAVRTGSGETLTMRARAIVGADGR
                     NSLVARRLGLRRGAPRHRRFAVMGRFSGARTPDDHGEMVVTADGYCGINPLPGGEANV
                     CFVLDPRRAARAPEGIAPAPAGAPLSQGFLPGRAHLERYVQWRIQSDPVVGQRLAGAR
                     LEGPLAAVGPIACGSRRAVGDAALLVGDAAEFFDPFTGEGVGTALAAGEIAAETLDLA
                     LSRGDLSRRALSAYERRRRRTFAGRFRVDRALQRILEHSGLTDFVAARLERQPVLADR
                     LARVTAGLASAGSLLRPDQVLRFLWA"
     gene            complement(3212..>3481)
                     /locus_tag="VFD06_11675"
     CDS             complement(3212..>3481)
                     /locus_tag="VFD06_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HZN04231.1"
                     /translation="RADRVPCSATKSLLGHPQGACGAMGLQATLLGLLGGYVPPTINY
                     ERPDPDCDLDYVPNRPRRHDIRLALCNCIGFGSKNAALVLRRWEP"
BASE COUNT          533 a         1295 c         1233 g          420 t
ORIGIN      
        1 ggtgtcgtac gagccgccgc gcgtcgcgtg gtccttgccg aacggctcga ccgtcaccgg
       61 gatgcgggcc cagcgggccg gccagtccac ccgccaggtc agcttgccgc cgccgcgcat
      121 cgacacggtg cccgtggcgc cgcagcggca ggcgtagtcg accgtttcgt ccttcgcgga
      181 gtagccgcgc acctgcgtct cggtcatgcg gcggcaggtc gcgcagatcg ggttgaacgg
      241 gctccagccg tcctccacct gccggccagt gcactcctgc aggatgcggg cgatggcgcc
      301 gcgcccctcg agcgcctgga cgatctgcgg cacgaggaag ccgctcttgt acatctggtc
      361 cgcgtagatc accttgacgt cgatgcgcag gcgccgcagc gagtcgagga acggctcgag
      421 gtaatgcgcc gcgtaggagg ggtgcttgcc gcacgggcag gggatctccg acagaggcgc
      481 cccgatgagc ggctcgtaga cgctccggtc gaggaacgga tagaccttcc ggagcgggtc
      541 gaagttgtcg gcgacgtagt cgagcaccac ctcgactccg cgctcgcgca gcgcccgcgc
      601 cacgacgtcg gcggtgatca cctcccgcag gttgccgatg tggatctccc ccgacgggga
      661 gatgccggtc gagatctcgt gccgtccgcc ctgcgtgggg atctgcgccg cgacctcgtc
      721 ggcccagtgc tggtcgcgca cgtgcgccgt catcgtgact ccggcttcgc caccgcgtgg
      781 aaggtcaccg tcaccttgtc atccaggacg aaccagagga tcttcggaat cgggatggag
      841 aattccgaga gcgtgaacga cacggtccca tcggccgtca ggagggcgcc atcatacccg
      901 accgtgaccg gaaacttgat ggtccgcgtc acaccgtgga ggtcgagctt cccctgcagg
      961 tcgaccggcc gcttctcccc cggccgcagc ggatccggct tcgttccggc cgacgaggac
     1021 tggaagcgga ctttcggata cgtcccggtc tcgaggtgcg agttgcgcat ggtgcggtcg
     1081 cggaggcggt tgccggtcac catcgtggcc gcgtccacct cgagcgccac gctcccaccg
     1141 cggtccgggt gctccggatc gatgcggacc gtccccgaga actgcgtcgc ggtcccctcg
     1201 acgacctcgc cgggccgcgt caccgtgaat cccagtttcg accccgccgc gtcgaacttg
     1261 aaggtccgca ccccggccgc cggcgcgggc gcctgaaccg ggggttcggc cgcgaacgcg
     1321 gccaggcccg cgatgcagcc gagggtggcg aagagcccca gcgccttcat cgggaccttc
     1381 tgccgggaag gaaccggggg cgcgatccca tcgccgcgac gtactccggt cgcgccatca
     1441 acgccgcctc ttcggtgcgc acgcgcgcga cgagcaccat gccgaaggcc gcgccgaaga
     1501 ccgcggcggt gagatacgcc ccgccggcga gcggcagggc gaggatctcc cccatcaccg
     1561 cgacgtagtt gggatggcgc aggtgacgat agggcccgcc ggaggcgacg cgcggccggg
     1621 ccgggaggat cacccgcatg gtccacgcat ccccgagcgc ccgcaccgcc gcccacttca
     1681 gccacttgga ggcggcgaac agcagcagca tggcgacgac cagggacggc gccggagagc
     1741 gggccagggc gagatgctcc caggccgccg ccgcgaaatg cagcgcgtag accgggacca
     1801 tccatccgaa cacggccggg gccacttccg tccctccttc cgccaggagc cgccgctcgt
     1861 tccggagcgc gacacggcgc tcgaggaccg ccgcgacgac cgccgcggcg acgaacggaa
     1921 gatacaacga ccccggaaga ctcacgccgg cgtcggcctc aggcccacag gaatctcagc
     1981 acctggtcgg gccgcaggag cgatccggcg gaggcgagcc cggcggtgac ccgcgccagc
     2041 ctgtcggcga gcaccggctg ccgctccagc cgcgccgcca cgaaatcggt cagaccggag
     2101 tgctccagaa tccgctgcag cgcccgatcg acgcggaaac ggccggcgaa ggtccggcgc
     2161 cggcgcctct cgtaagcgct cagggcgcgg cgcgacagat cgccgcgcga caacgccagg
     2221 tcgagggtct cggcggcgat ttcgcccgcc gcgagcgccg tgccgacccc ttcgccggtg
     2281 aacggatcga agaactccgc cgcgtccccg acgagcaggg ccgcatcacc gacggcgcgg
     2341 cgcgacccgc aggcgatcgg gccgacggcc gcgagggggc cttcgagccg ggcccccgcg
     2401 agcctctggc cgaccaccgg atccgactgg atcctccact gcacgtagcg ctcgagatgg
     2461 gcccggcccg ggaggaatcc ctgcgacagc ggcgcgcccg ccggagcagg cgcgatccct
     2521 tccggagcac gcgccgcacg ccgcggatcg aggacgaaac agacgttcgc ctccccgccc
     2581 ggcagcgggt tgatgccgca atagccgtcg gcggtgacga ccatctcgcc gtgatcgtcc
     2641 ggagtgcggg cgccggaaaa gcgccccatc accgcgaagc gccgatgccg gggggcaccg
     2701 cgccgcagcc cgagccgccg cgcgacgagc gagttccggc cgtcggcgcc gacgatcgca
     2761 cgggcgcgca tcgtcaaggt ctcccctgag cccgtccgca cggcgaggac gtaaccaccg
     2821 ccgggatgcg gcgtgacatc ggtgacccgc gtcccttcga gcacctcggc ccccgcgcgt
     2881 cgggcggcgc ggagaagcac tgcgtcgaga tcggggcggc ggagcgcgta cccgggcccg
     2941 gcctcggctc cgaagcggcc gagcaccctc gccccgcccg gggcgaccac cgccatgccg
     3001 atcgggcgcc gcggcgcgag cgcctccacg tcccggagcg ctccgaggcg ctcgagacgc
     3061 ggcgccaccg acggcgggag gaattcgccg cagatcttct cgcgcgggaa gcgggccgcc
     3121 tcgcacacga gcacgttccg accgaggcgc gccaggaacg cggcggtcga ggcccccgcc
     3181 ggacccccgc ccacgacgac cacgtcgcgg gtcacggctc ccaccggcgc agcacgagcg
     3241 cggcgttctt cgaaccgaag ccgatgcagt tgcagagcgc cagccggatg tcgtggcggc
     3301 gcggccggtt cgggacgtag tccagatcgc agtcggggtc cgggcgctcg tagttgatcg
     3361 tgggaggaac gtatccgccc agaagaccga gcagcgtcgc ctgcagcccc atcgcgccgc
     3421 acgccccctg cgggtgtccg agaagcgact tggtggccga gcacggcacg cgatccgcgc
     3481 g
//
LOCUS       DASRIQ010000478         4951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3861822, whole genome shotgun sequence.
ACCESSION   DASRIQ010000478 DASRIQ010000000
VERSION     DASRIQ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3861822"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFD06_11680"
     CDS             complement(<1..881)
                     /locus_tag="VFD06_11680"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04232.1"
                     /translation="MTGRAADSAAPLADDGDRPPHAPERGWSWRLLGRFHITGIFWYR
                     VHGFILRVVPEPLLEAVAACFAALFFVCLRRIRSAVAANLEAVLGPCGWRERQRRIFR
                     VIWSFGWCMNERTERLTTDRPFVIETEALDVWLEATRGGRGLILITAHIGNYEVGSMM
                     PAQKEGRRVHLVREPEGDPEAQAYVKGVLDRFSQHRFEEHFATGDPSLGLALLAALRR
                     GDIVALQGDRPRTAAPAIPVRLFGRRFELPHGPAALARVAGVPMLPVFVFREGRRRYR
                     IVFRPPIEVERTAPRETS"
     gene            983..2461
                     /locus_tag="VFD06_11685"
     CDS             983..2461
                     /locus_tag="VFD06_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04233.1"
                     /translation="METRGAADYRIEPEFLGPLGDRPLAEAAGKSSPDDLFPGASKFL
                     SARVQRKRREQWNAVRPLVKRLLAPGEHILYVAYAQQVPGWLDHVGLKHWIYAYSQVI
                     LVVTDQRIIEALLNFRANGPGTRLRSFTYRSLRDLKLKFGKLTAKPANGKPHAWRALS
                     GGDRKLLGLLLPRLLPRLLPEGTGHAEAAPQWHCPKCGAAVAPDPEACAGCRTLFRST
                     RFAVMLSLAFPGGGLFYLGHPFLATLAMLGEAMIYFLGLTLLLRAPASEGPVAALVTG
                     AFMLAMMKLVMANVVRVLGRRSIPEPEGRRVRAGRLAVAGGALLMLLTAGAFPLAAAA
                     RPRLDHDLDVTAADGSWHGSRSRSEWKAFKDDSDARSQWTDDRTGAMVAVFAYPRSVL
                     DSTSEFRRQYMGAMKAQTARTLYEDTDVPAPFQGFRHASGIRTKDGGEVALMSWFLED
                     AEAHDLHEVRIVVGIDEVEAGEALVEDFLSQAKFVDAVAPKP"
     gene            2513..4027
                     /locus_tag="VFD06_11690"
     CDS             2513..4027
                     /locus_tag="VFD06_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003237515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HZN04234.1"
                     /translation="MDSRPVRLDRDAIVAGPGFNRWLVPPAALAVHLSIGQAYAYSVF
                     KLPLTRLLGVTSSAPGDWSQTQIALVFTVFIVFLGLSAAVFGRWVETAGPRKAGIVAA
                     CCWSGGYLVAALGVATHRLWLLYLGNGVIGGCGLGLGYISPVSTLVRWFPDRRGMATG
                     MAIMGFGGGALIGAPLADLLMRRFATPVSTGVWQTFVAMGAIDFAAMLCGAFGYRLPP
                     PGWRPPGAAAATRVAGSAVEMHVRDAWRTPQFALLWIVLCANVSAGIGLLEQASPMIQ
                     EVFKGAVSASAAAGFVGLLSLFNIAGRFGWASLSDRIGRPRTYGLFFFIGALLYAALP
                     VASRAGSVALFVAIVCVIMTMYGGAFATIPAYLADLFGTQFVGAIHGRLLTAWSVAGV
                     VGPLLVNVIRQRRIDAGAAPADAYNTTMWVLAGLLVVGFFANLKVRPVEAGRGGARRK
                     GASSAPVPAVAPGASARPFESRRWPLVFAVWALVGLPFLWGVLQTLEKAARLFL"
     gene            complement(4029..4634)
                     /locus_tag="VFD06_11695"
     CDS             complement(4029..4634)
                     /locus_tag="VFD06_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HZN04235.1"
                     /translation="MPAAAGRSGAPARERWGAILLVLAMLAITAFVELRMGRSPFGPD
                     GRFGWWEADIWSSANSQRVADAYSFSHFIHGIVFFGLLWLVARRLPIGHRLMIALLLE
                     GGWEILENSPLIIDRYRAATIAVGYVGDSVLNSCSDVVMMALGFLFARRVPVRASVAT
                     AVAMELFCLAWVRDNLTLNVVMLLHPIEAIKAWQAAGHLLP"
     gene            complement(4652..>4951)
                     /locus_tag="VFD06_11700"
     CDS             complement(4652..>4951)
                     /locus_tag="VFD06_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN04236.1"
                     /translation="PAWAQPPAPPASPERRAIQEGHLANIQRLADAGKLLLAGPFTDG
                     GELRGLFVFQVASLEEAKAMCDSDPAVKAGRLRVELHPWYSAKGISVPKPATPAQ"
BASE COUNT          685 a         1732 c         1785 g          749 t
ORIGIN      
        1 gacgtctcgc gcggtgcggt gcgctccacc tcgatcggcg gacggaagac gatgcgatag
       61 cgccggcggc cttcgcggaa gacgaacacc ggcagcatcg ggacgccggc gacgcgggcg
      121 agcgccgccg gtccatgcgg cagctcgaac cgcctcccga acaggcggac cgggatcgcc
      181 ggcgccgcgg tccgcgggcg gtccccctgc agcgccacga tgtccccgcg gcgcagcgcg
      241 gcgagcagcg ccagcccgag ggaggggtcg ccggtggcga agtgctcctc gaagcgatgc
      301 tgcgagaacc ggtccagcac tcccttcacg tacgcctgcg cctcggggtc tccctccggc
      361 tcgcgcacca ggtgcacgcg ccgcccctcc ttctgcgccg gcatcatcga gcccacctcg
      421 tagttgccga tgtgcgcggt gatcaggatg aggccgcggc cgccacgcgt cgcctcgagc
      481 cagacatcga gcgcctcggt ctcgatgacg aagggccggt cggtggtgag gcgctcggtg
      541 cgctcgttca tgcaccagcc gaacgaccag atcacccgga agatgcggcg ctgccgctcc
      601 cgccagccgc acggaccgag caccgcctcg aggttcgccg ccacggccga gcggatacgc
      661 cgcaggcaca cgaagaaaag agcggcgaag caggccgcca ccgcctcgag gagcggctcc
      721 gggacgacgc ggaggatgaa gccgtgaacg cggtaccaga agatgccggt gatgtggaac
      781 cggccgagga gccgccacga ccagccccgc tccggcgcgt ggggcggacg gtcgccgtcg
      841 tctgccagcg gcgcggcgct gtccgcggcc cgtcccgtca tgtttccgac cctcctccgc
      901 gaaccgcgcg cggcgcgatt gcgaaattct aattgacctc ggagccgccg cgtctaagtt
      961 gagatgtcgg gggggactga ttatggagac acgcggcgcg gcggattaca gaatcgaacc
     1021 ggagttcctc ggaccgctcg gcgatcgccc gctggcggag gcggcgggca agtcgtcccc
     1081 cgacgacctg ttcccaggag cctcgaagtt cctcagcgcc cgcgtgcagc gcaagcgccg
     1141 cgagcagtgg aacgcggtcc ggccgttggt gaagcgtctc ctcgccccgg gcgagcacat
     1201 cctctacgtc gcgtacgccc agcaggtgcc gggatggctc gatcacgtgg gcctcaagca
     1261 ctggatctac gcctacagcc aggtgattct ggtcgtcacc gatcagcgga tcatcgaagc
     1321 gctcctcaac ttccgcgcca acggccccgg cacgcggctg cgatccttca cgtaccgatc
     1381 gctgcgcgat ctcaagctga agttcggcaa gctgacggcc aagcctgcga atgggaagcc
     1441 gcacgcctgg cgggccctct cgggcggcga ccggaaactc ctcggcctcc tgctcccgcg
     1501 cctgctgccg cgcctgctgc cggaggggac cggccacgcc gaagccgcgc cgcaatggca
     1561 ctgcccgaag tgcggcgccg cggtggcgcc ggaccccgag gcctgcgccg gctgccggac
     1621 gcttttccga tcgacgcgct tcgcggtcat gctgtcgctg gcctttccgg gcggcgggct
     1681 cttctatctc gggcatccgt tcctcgccac gctcgcgatg ctcggagagg cgatgatcta
     1741 cttcctcggg ctcacgctgc tcctcagggc tccggcgagc gagggaccgg tggcggccct
     1801 ggtgacgggg gccttcatgc tggcgatgat gaagctggtg atggcgaacg tggtgcgggt
     1861 tctcggcagg cgctcgattc cggagccgga gggacgacgc gtccgcgccg gccggctggc
     1921 ggtcgccggg ggggccctcc tgatgctgct cacggcgggg gcgtttccgc tcgcggccgc
     1981 ggcgcggccg cggctcgatc acgacctcga cgtgacggcc gcggacggtt cctggcacgg
     2041 cagccggagc cgttcggagt ggaaggcgtt caaggacgat tccgacgccc gctcgcagtg
     2101 gaccgacgat cgcaccggcg cgatggtggc cgtcttcgcc tatccgcgca gcgtcctgga
     2161 cagcacctcc gagttccgtc gccagtacat gggcgcgatg aaggcgcaga cggcgcgcac
     2221 tctttacgag gacaccgatg tgccggcccc gttccagggg ttccggcacg cctcggggat
     2281 ccggacgaag gacggcggcg aggtggcgct gatgtcctgg ttcctcgagg acgccgaagc
     2341 gcacgacctg cacgaagtgc ggatcgtcgt ggggatcgac gaggtggagg ccggcgaggc
     2401 cctcgtcgag gacttcctca gtcaggcgaa gttcgtcgac gcggtcgcgc cgaagccctg
     2461 aaggcgcgcg tcgacgtgag cactccggag ggcgcctgag gggccgggcc ggatagactc
     2521 ccgccccgtg cgcctcgacc gcgacgccat cgtcgccggc cccggcttca accgctggct
     2581 ggttccgccg gccgcgctgg cggtgcacct gtcgatcggg caggcgtacg cctacagcgt
     2641 cttcaagctg cctctgacgc ggctgctcgg cgtcacgtcc tccgcgcccg gcgactggtc
     2701 gcagacgcag atcgcgctgg tcttcaccgt cttcatcgtt tttctcgggc tctcggcggc
     2761 cgtcttcgga aggtgggtgg agacggccgg tccgcgcaag gccgggatcg tcgccgcatg
     2821 ctgctggagc ggcgggtacc tcgtggcggc gctcggcgtc gcgacgcacc ggttgtggct
     2881 cctctacctc ggcaacggtg tcatcggcgg gtgcggactc gggctcggat acatctcgcc
     2941 ggtctccaca ctcgtcaggt ggttcccgga tcgccgtggc atggcgaccg gcatggcgat
     3001 catgggattc gggggcggtg cgctgatcgg ggcgccgctg gcggatctcc tgatgcggcg
     3061 gttcgccacg ccggtctcga ccggtgtgtg gcagaccttc gtcgccatgg gcgcgatcga
     3121 cttcgccgca atgctgtgcg gcgcgttcgg ctaccgcctc ccgccgcccg ggtggcgccc
     3181 gcccggcgcc gccgccgcga cgcgcgtggc ggggagcgct gtcgagatgc acgtgcgcga
     3241 cgcctggagg acgccgcagt tcgcgctcct ctggatcgtg ctctgcgcca acgtcagcgc
     3301 cggcatcggc cttctcgagc aggcgtcgcc gatgatccag gaggtgttca agggagcggt
     3361 gagcgcttcg gccgccgccg ggttcgtcgg gttgctgtcc ctcttcaaca tcgcgggaag
     3421 gttcggctgg gccagcctct ccgatcggat cgggcgcccc cggacctacg gcctgttctt
     3481 tttcatcggc gcgctgttgt atgcggcgct gccggtcgcc agccgcgccg ggagcgtggc
     3541 cctcttcgtc gccatcgtct gcgtgatcat gacgatgtat ggaggcgcgt tcgcgaccat
     3601 cccggcgtac ctcgcggatc tcttcggcac gcagttcgtc ggggcgatcc acggccggtt
     3661 gctgacggcg tggtcggtcg cgggagtggt cgggccgctg ctcgtgaacg tcatccggca
     3721 gcggcgcatc gacgccggcg ccgcacccgc cgacgcctac aacacgacga tgtgggtgct
     3781 ggccgggctg ctggtcgtcg gatttttcgc gaacctcaag gtgcggccgg tggaagccgg
     3841 gcgcggcggc gcacgccgga agggcgcgtc atcggcgccg gtcccggccg tcgcaccggg
     3901 ggcatcggcc cggccgttcg aatcgaggcg ctggccgctc gtgttcgcgg tctgggcact
     3961 cgtggggctg ccgttcctct ggggcgtgct gcagaccctg gagaaagcgg cgcggctgtt
     4021 cctctgaatc agggaagcag gtggccggcc gcctgccagg ccttgatcgc ctcaatcggg
     4081 tggaggagca tcacgacgtt gagcgtcagg ttgtcgcgca cccaggcgag gcagaacagc
     4141 tccatggcca cggccgtcgc gacgctggcc cgcaccggca cgcggcgcgc gaacaggaag
     4201 ccgagcgcca tcatcacgac gtcgctgcac gagttcagga cgctgtcgcc cacgtacccc
     4261 accgcgatcg tggcggcgcg gtagcggtcg atgatgagcg gagagttctc gaggatctcc
     4321 cagcccccct cgagcaggag cgcgatcatc agccggtgac cgatcggcag ccggcgggcg
     4381 accagccaca ggagcccgaa gaagacgata ccgtggatga agtgggagaa ggagtacgcg
     4441 tcggcgacgc gctgcgagtt cgcgctcgac cagatgtcgg cctcccacca gccgaagcgg
     4501 ccgtcggggc cgaaggggga gcggcccatg cgcagctcga cgaacgccgt gatcgcgagc
     4561 atcgccagga ccaggaggat cgccccccac cgctcccgcg ccggagcccc gctccggccg
     4621 gccgccgccg gcatgtcggc cacagcgtcc cctactgcgc cggcgtcgct ggtttcggca
     4681 cgctgatccc cttcgccgag taccacgggt gcagctcgac gcgcagccgc cccgccttga
     4741 cggcgggatc gctgtcgcac atcgccttcg cctcctcgag agaagcgacc tggaagacga
     4801 acagaccgcg cagctcgccg ccgtcggtga acgggccggc gaggagcagc ttgcccgcgt
     4861 ccgccaggcg ctggatgttg gcgagatgcc cttcctggat cgcccggcgc tccggtgacg
     4921 cggggggagc gggcggttgc gcccaggccg g
//
LOCUS       DASRIQ010000479         9254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2520030, whole genome shotgun sequence.
ACCESSION   DASRIQ010000479 DASRIQ010000000
VERSION     DASRIQ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520030"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFD06_11705"
     CDS             complement(<1..496)
                     /locus_tag="VFD06_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZN04237.1"
                     /translation="MTSGRLLGSLGATLRRVRLPLLAVAWLLVAASPAGAGSDIQIDF
                     DRAFDFSTIHSYAWKDDPKVSLSEDNPFLHSRIVEIVDGRMYRAGIKKVEEDPDAYVA
                     YRVAINSDMAIDAAEYGYFYPGTWYWSPVAWSWATPNMPDERTYTKGTLLIEIWTART
                     KQIVW"
     gene            601..2775
                     /locus_tag="VFD06_11710"
     CDS             601..2775
                     /locus_tag="VFD06_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN04238.1"
                     /translation="MNRRLAFPLAAALLIAAAPLPAADVAREVEPRPPVAAKKPHPVV
                     SPNGTRDDPYYWLRDDTRSKPEVLNYLKDENAYYAAESAAYRDLTETLSKEILGRLKQ
                     DDSSVPFKYRDYLYYSRYEAGKEYPIHARRPLGSDKEQVLVDANAQAEGKSFYSLGRR
                     AVSPKQDLLGYLEDTTGRRQFTLRVRDIATGKDRPESIPGLSGAVVWASDNKTLFYVE
                     NDPVTLLSTRVKRHVLGTDPKSDPVVYEEKDHSFYLTVDESGDERFVLIRLRSTTETE
                     MRVIDAAEPAAAPKVFAARTKDHLYDADHIAGRWIVRTNRNAPNYRMMTVPDATAFGG
                     PDAWRDLLPYDKEVFVEDFALFRDYLVINERSEGLLRLRVRPWSDLQASKVIQSDEPA
                     YAEELSINPEQGTELLRYTHDSLVTPRRTFEANMRTGERKLLKEQPVLGGFKAENYET
                     ARVWAVARDGTKVPVSLAWRKGFKRDGSAPMYQTAYGAYGYSSDPSFEIEAVSLLDRG
                     FVYALAHIRGGQEMGRAWYDDGHLLRKKNSFTDFIDVTDFLVKEKYAAPDKVFAMGGS
                     AGGLLMGAVANMAGEKYRAIVAHVPYVDAVTTMLDETIPLTANEFDEWGNPKEKKYYD
                     YILSYSPYDNVRARQYPALYVTTGLHDSQVQYFEPAKWVAKLRALKTDTNPLLFKINM
                     SAGHGGRSGRFESLKETAEEYAFLLNLIGLRGKVSPVAAAGG"
     gene            complement(2818..3354)
                     /locus_tag="VFD06_11715"
     CDS             complement(2818..3354)
                     /locus_tag="VFD06_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN04239.1"
                     /translation="MRIKEIMSTPAVTCRAGEPLDAAARLMWECDCGVIPVVDEAGQL
                     AGIVTDRDIAMAAWMKGRTLKEIPIAEAMARQVVTCRPDDSIEDAERLMSGQQIRRLP
                     VVDGEGHPVGVLSLGDIARCTALKGDGGDQDVVRSLAEITKPRRPDQAARAGASTTAP
                     RDRGVREQAGRRAAGPSM"
     gene            3496..5928
                     /locus_tag="VFD06_11720"
     CDS             3496..5928
                     /locus_tag="VFD06_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN04240.1"
                     /translation="MTLPQGTRLGPYEIVAPLGSGGMGEVYRAHDARLRRDVAIKVLP
                     GKFSDDPERLRRLQQEAQAAGALNHPNILTIYDIGTHEGVAYIVSELLEGRTLRALLA
                     AGPVPPRRALDIALQIARGLDAAHARGIVHRDLKPENLFVTQDDRVKILDFGLAKLRD
                     ETVAGAAAPSAEEITSLPTRHQGTAPGVVLGTLGYMSPEQVRGQPTDGRSDIFSFGAI
                     LYELLSGRRAFQRDTPADTISAILTGEPADPAPVPGRGVPPGLDRILRHCLEKDPARR
                     FQSAHDLAFDLETMSGGHTAGGASPAQPEWAAASRRLSFWGAAGAAVVTALVAGAYFV
                     GRPPGAGAAIDSIAVLPFVNAGQDPEMEYLSDGITESLIHSLSKLPHLRVMSRNSVFH
                     YKGKEVDAREAGAALGVKAVLTGRVMERGETLSINAELVDVRDNSHLWGAQYGGKSSD
                     VLSVQQEIARQISDNLRVRLTGDEKSRLAKRPTESNEAYQLYLKGRFFWNQRGQGLLK
                     ATEYFDQALNLDPNYAQAHAGSADALTVLAFYGFRPAREAFPKAKARAERALAIEPSL
                     GEAHATLGYIAFHYDWDLGAAEREYLRGIELAPNHPPAHYWYAIMLSTKGDWDGATAE
                     MEKAMSLDPLSPFVNIQAGWLHLRRGAFDEAVDRIRKGMELNPSIVIGHLLLGHVQTL
                     RGRFEEGIAEMSEALRLSDGDAWVKGSLAYAFAASGEGKRARTLLEELLEGPTPAGFR
                     RSYPIALAYAGLGDRDRAFTFLDKSREERDPMVSVVRTDHLLDGLHSDPRWLPYLRSL
                     GLLSDSELAKIR"
     gene            5999..8416
                     /locus_tag="VFD06_11725"
     CDS             5999..8416
                     /locus_tag="VFD06_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04241.1"
                     /translation="MRGFADDLGLACRRLLRAPGFTAVTVAALALGIGLNTAIFSVVN
                     AALLRPLPYPEPERLVEISLPTASGEAHGPVSFVDFEDWRGQSHVFSSIAAFSTISGL
                     NLRGEGDREPEQLRTAFVDPAFFATLGVVPAIGRPLVAADNEPGRNQAVVISHRLWQR
                     RFDGDPAIAGRAITLDEDPFTVAGVMPESFRFPDASIEVWAPESLIEASAAPRRRDNR
                     FQRVIARLDRGVSVERARLEMNLIASRLAQEFPETNTGRDTVTLVSLHESLVGPRLRA
                     AVLVLLAGVGLVLLIACANVAHLLIARIASRRRETAIRLALGATPSGIVRLHVAESLV
                     VSLAGGLLGLLTAAWTLQLVRGGVLPYLPAQMDVVLDRRVLGFALLTSIAAALLCGVL
                     PALRGDRSVPHQDLKEGARGAGAGGERRRLREILVISEMALAVVLVVAAGLALKSFRR
                     LSGVDPGFDPDAVLTVAMNASGARYPERAQFVEFQRRVVERVQHIPGVVAAGSTRNLP
                     LLGTGESWPVVPEGGAPVTPGNEPEVAVHPVSAGFFATLRIPLLEGRPFTEPESGPPV
                     AIVSRALARRFWPDGGAVGSALLVGETRLAVVGVVGDVRQATLEQEGEAALYLPSGLS
                     PRRGFTLVIRTTGPPRALESLVRSAIHEIDPQQPILRIAAMREVLGSALAQPRLMSLL
                     LGAFGTLAFALALLGVYGVIALMVSLRTYEFGVRMALGATAGSVRRMVLRRAILLGAC
                     GIGAGSLAGLGLTRLLRSQLYAVSPADPPTFLLAAAALLVACLFAGDGPARRATRIDP
                     LVALRSE"
     gene            8493..>9254
                     /locus_tag="VFD06_11730"
     CDS             8493..>9254
                     /locus_tag="VFD06_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04242.1"
                     /translation="MKKPRPTSRLRRAVPATVLFCASLAAPGGAHADRTDIQGSVPVL
                     VKSRGPLTAETAAAIGSHGTHITYLWPQIDAMAMTVNPSRLEELASDPAVAFVELDQQ
                     GSAPGAGPSGSGATAPMTAVPLPQADAPIVPWNLDMADVFGTGYDGAGVTVAVVDSGL
                     PQNWEEFLPEGVVDTEHAAGFGAEGWGDFHAPLHAIRGAGGHIGLFPHGLAVSSIIAG
                     FPSEAGPVGGAAPGARILPVRVLNQFNFGWFSWFAA"
BASE COUNT         1364 a         3268 c         3210 g         1412 t
ORIGIN      
        1 gccagacgat ctgcttcgtg cgcgcggtcc agatctcgat cagaagagtc cctttcgtgt
       61 aggtccgctc gtccggcatg ttcggcgtgg cccaggacca ggccaccggg gaccagtacc
      121 acgtccccgg gtagaagtat ccatattccg ccgcgtcgat cgccatgtcg ctgttgatgg
      181 cgacccggta ggcgacgtag gcgtccggat cctcctccac cttcttgatg cccgcgcggt
      241 acatccggcc gtcgacgatc tcgacgattc ggctgtgcag gaacgggttg tcctccgaca
      301 acgagacctt cgggtcgtcc ttccaggcgt acgagtggat ggtcgagaaa tcgaacgcgc
      361 ggtcaaagtc gatttggatg tccgatcccg cgccggccgg cgaggcggcg accaggagcc
      421 aggccaccgc aaggaggggg agccgcaccc ggcgcaaggt cgcgccaagg cttccgagga
      481 gacgtcccga ggtcatgtgg cccggagtgt actgcggaag gggccgcgcg aggggctcac
      541 cgggcccgaa ggatgaggta cgatgcggcc cattcccgga taccgtcagg aggggcctcc
      601 atgaaccgtc gactcgcgtt cccgctcgcc gcggcgcttc tgatcgccgc cgcacctctg
      661 cccgccgccg acgttgcgcg cgaggtcgaa ccacggcctc cggtcgcggc gaagaagccc
      721 caccccgtgg tctcccccaa cggcacgcgg gacgatcctt actactggct gcgcgacgac
      781 acccgttcca agccggaggt gctgaactac ctcaaggacg agaacgccta ctacgccgcc
      841 gagagcgccg cctaccggga cctgaccgag acgctgtcga aggagatcct cgggcgactc
      901 aagcaggacg acagcagcgt ccccttcaag tatcgcgact acctctatta ctcgcgttac
      961 gaggcgggca aggagtaccc gatccacgcc cggcggccgc tcggatccga caaggagcag
     1021 gtcctcgtgg atgccaacgc gcaggccgag gggaagtcgt tctactcgct ggggcggcgc
     1081 gccgtcagcc cgaagcagga cctcctcggg tacctggagg acacgaccgg ccgacggcag
     1141 ttcacgctgc gcgtccgcga catcgcgacc gggaaggatc ggcccgagtc gatccccggc
     1201 ctgagcggcg cggtcgtctg ggcgagcgac aacaagacgc tcttctacgt cgagaacgac
     1261 ccggtcacgc tcctctccac gcgggtgaag cggcacgtcc tcgggaccga cccgaagtcc
     1321 gacccggtcg tgtacgagga gaaggatcac tccttctacc tgaccgtgga cgagtcgggg
     1381 gacgagcgct tcgtgctgat ccgcctgcgc agcaccaccg agaccgagat gcgggtgatc
     1441 gacgccgccg aaccggccgc ggcgccgaag gtgttcgcgg cgcggacgaa ggaccacctc
     1501 tacgacgccg atcacatcgc cgggcgctgg atcgtccgga cgaaccgcaa cgcccccaac
     1561 tacagaatga tgacggtgcc ggacgccacg gccttcggcg ggccggacgc ctggcgcgat
     1621 ctcctcccgt acgacaagga ggtcttcgtc gaggatttcg ccctgttcag ggactacctc
     1681 gtgatcaacg agcgcagcga gggtctgctg cgcctgcggg tgcggccctg gtccgacctt
     1741 caggcgtcca aggtcatcca gtccgacgag ccggcctacg ccgaagagct gtcgatcaac
     1801 ccggagcagg ggacggagct gctgcgctac acccacgact ccctggtcac cccgcgacgc
     1861 accttcgagg cgaatatgcg gacgggggag cgcaagctcc tgaaggagca gccggtcctc
     1921 ggcggcttca aggcggagaa ctacgagacg gcgcgggtgt gggccgtggc gcgcgacggc
     1981 acgaaggtgc cggtgtcgct ggcgtggcgc aagggattca agcgggacgg ctcggccccg
     2041 atgtaccaga cggcgtacgg ggcatacggc tactcgtcgg acccgtcgtt cgagatcgag
     2101 gcggtgtcgc tcctcgatcg cggcttcgtg tacgcgctcg cccacatccg cggcgggcag
     2161 gagatggggc gcgcctggta cgacgacggg cacctgctca ggaagaagaa ctccttcacc
     2221 gacttcatcg acgtcaccga cttcctcgtg aaggagaagt acgccgcgcc ggacaaggtg
     2281 ttcgccatgg gcgggagcgc cggcgggctg ctgatggggg cggtggccaa catggcgggg
     2341 gagaagtacc gggccatcgt cgcgcacgtg ccgtacgtcg acgccgtcac caccatgctc
     2401 gacgagacga tcccgctcac cgcgaacgag ttcgacgagt gggggaaccc gaaggagaag
     2461 aagtactacg actacatcct ctcgtactcg ccgtacgaca acgtgcgcgc ccgacagtac
     2521 ccggcgctct acgtcacgac cgggctgcac gattcgcagg tgcagtactt cgagccggcc
     2581 aagtgggtcg cgaagctgcg agccctgaag accgacacca accccctgct cttcaagatc
     2641 aacatgtccg ccggccacgg cggccgctcc ggccgcttcg agagcctcaa ggagaccgcc
     2701 gaggagtacg ccttcctcct gaatctcatc ggcctgcgcg ggaaggtgtc tcccgtggcc
     2761 gctgcgggcg gctgaagcgc ggagacgaac cccgcctcac agcgagtccg agttgcccta
     2821 catcgacggc cccgcggcgc ggcgaccagc ctgctcccgc accccgcggt cacgcggcgc
     2881 cgtcgtggag gctccggcgc gcgccgcctg gtccggccgg cgcggtttcg tgatctccgc
     2941 caggctgcgg accacgtcct gatccccgcc atcccctttc agggccgtac agcgggcgat
     3001 atccccgagt gagagcaccc cgacagggtg cccctcgccg tccacgaccg gcaggcggcg
     3061 gatctgctgg ccgctcatca ggcgttccgc gtcctcgatg ctgtcgtccg gccggcaggt
     3121 gacgacctgc cgcgccatcg cctccgcgat cggaatctcc ttgagcgtcc ttcctttcat
     3181 ccacgccgcc atcgcgatgt cccgatcggt cacgatgccg gcgagctgcc cggcctcgtc
     3241 cacgaccgga atcactccgc agtcgcactc ccacatcagc cgggcggccg cgtcgagggg
     3301 ctcccccgca cgacaggtga ccgccggcgt gctcatgatc tccttgatgc gcatgttcgt
     3361 ctccttcttc ggaacggatc cggctcagga tgagggagcg accgcacggt tgtcgatggc
     3421 gagttctact gaggcgctgc acttgcatgc cggcccggag atccgggcta agatgccgcg
     3481 atttcgggtc ggggaatgac gctccctcaa ggcacgaggc tgggtccgta cgagatcgtc
     3541 gcgccgctgg gctccggcgg gatgggggag gtgtaccgcg cgcacgacgc gcgcctgcgc
     3601 cgcgacgtgg cgatcaaggt gctccccggg aagttctccg acgatccgga gcggctgcgg
     3661 cgcctgcagc aggaggcgca ggcggcggga gcgctcaacc acccgaacat cctgacgatc
     3721 tacgacatcg gcacgcacga gggggtcgcc tacatcgtct cggagctgct cgagggacgc
     3781 acgctccggg cgctcctcgc ggccgggccg gtcccgccgc gccgcgcgct cgacatcgcc
     3841 ctgcagatcg cccgcggcct cgacgccgcg cacgcgcgcg gcatcgtcca ccgcgatctc
     3901 aagccggaga acctgttcgt cacccaggac gaccgggtga agatcctgga cttcgggctg
     3961 gcgaagctgc gcgacgagac ggtcgccggc gccgcggccc cgtcggcgga ggagatcacc
     4021 agcctgccga cgcggcacca ggggaccgcg cccggcgtcg tgctggggac gctcggctac
     4081 atgtcccccg agcaggtgcg cggccagccg accgacggac gctccgacat cttctcgttc
     4141 ggcgcgatcc tctacgagct gctcagcggc cggcgcgcct ttcagcgcga cacgccggcc
     4201 gacaccatca gcgccatcct gaccggcgag ccggccgatc ccgcgccggt cccgggccgc
     4261 ggcgtcccgc cggggctcga ccggatcctg aggcactgcc tcgagaagga cccggcgcgc
     4321 cgcttccagt cggcgcacga tctggcattc gatctcgaga cgatgtccgg gggacatacg
     4381 gccggcgggg cgtcacctgc gcagccggag tgggcggcgg cctcccgccg cctctccttc
     4441 tggggcgccg ccggggccgc ggtcgtcacg gcgctggtcg ccggcgcgta cttcgtcggg
     4501 cgtccgccgg gggcgggggc ggccatcgac tccatcgccg tgctgccgtt cgtcaacgcc
     4561 gggcaggatc cggagatgga gtacctgagc gacgggatca ccgagagcct gatccacagc
     4621 ctgtcgaagc tgccgcacct ccgggtgatg tcgcgcaact cggtttttca ctacaagggg
     4681 aaggaggtgg acgcgcgcga ggcgggggcg gcgctcggcg tgaaggcggt cttgaccggc
     4741 cgggtcatgg agcgggggga gacgctatcg atcaatgccg agctggtgga cgtgcgcgac
     4801 aacagccatc tctggggcgc ccagtacggg ggcaagtcgt ccgacgtcct gtcggtgcag
     4861 caggagatcg cccggcagat ctccgacaac ctgcgcgtcc ggctgacggg ggacgagaag
     4921 agccgcctgg cgaagcgccc gaccgaaagc aacgaggcgt accagctcta tctcaaggga
     4981 cgcttcttct ggaaccagcg gggacaaggg cttctgaagg cgaccgagta cttcgatcag
     5041 gcgctgaacc ttgacccgaa ctacgcgcag gctcacgctg ggtccgccga cgccctgacg
     5101 gtgctggcct tttacggctt tcgtcccgcc cgcgaggcgt ttccgaaagc gaaggcgagg
     5161 gcggagcgcg ccctggcgat cgagccgtcg ctgggcgagg cgcacgcgac cctcggctat
     5221 atcgccttcc actacgactg ggatctgggg gcggccgagc gcgagtacct gcgcggcatc
     5281 gagctcgccc ccaaccatcc gccggcgcat tactggtacg ccatcatgct gtcgacaaag
     5341 ggcgactggg acggtgcgac cgccgagatg gagaaagcga tgtcgctcga tccgctctct
     5401 ccgttcgtga acatccaggc ggggtggctt cacctgcgcc ggggagcgtt cgacgaggcg
     5461 gtcgatcgga tccgcaaggg gatggagctc aacccgtcga tcgtcatcgg ccacctgctc
     5521 ctgggccatg ttcagacgct gcgcgggcgg ttcgaggaag ggattgccga gatgagcgaa
     5581 gccctgcgac tctccgacgg ggacgcctgg gtgaagggtt ccctcgcgta cgccttcgcc
     5641 gcgtcgggag aagggaaacg ggcccggacg cttctcgagg agctgctgga ggggccgacg
     5701 ccggcagggt tccggcgctc ctatccgatc gcgctcgctt acgccggact cggcgaccgg
     5761 gatcgcgcct tcacgttcct cgacaagtcg cgtgaggaga gagaccccat ggtgtcggtc
     5821 gtgcgcaccg atcacctcct cgacgggctg cactccgacc cgcgctggct tccgtacctg
     5881 aggtcgctcg gcctcctctc cgactcggag ctggcgaaga tccgctgagc cggcgaggcg
     5941 gccgggtttt ttgcttttcg gggacaaccc gaaccgttgc ggtatcgttc tctcccgtat
     6001 gcgtggattc gccgacgatc tgggcctcgc ctgccggcgc ctgctgcgcg cacccgggtt
     6061 cactgcggtc accgtggcgg cgctggcgct cggcatcgga ctgaacaccg ccatcttcag
     6121 cgtcgtcaac gcggccctgc tgcgcccgct gccctatccc gagccggagc ggctggtgga
     6181 gatctccctg ccgaccgcct cgggggaggc gcacgggccg gtgtcgttcg tcgatttcga
     6241 ggactggagg ggacagagcc acgtgttctc ctcgatcgcg gcattcagca ccatctcggg
     6301 gctgaacttg cgcggggagg gggatcggga gccggaacag ctccgcacgg ccttcgtgga
     6361 ccccgcattc ttcgcgacgc tcggcgtcgt tccggcgatc gggcgcccgc tcgtcgccgc
     6421 cgacaacgag ccggggcgca accaggcggt cgtcatcagc catcgtctct ggcagcggcg
     6481 cttcgacggc gacccggcga tcgccggccg cgccatcacg ctggacgagg atccgttcac
     6541 cgtggccggg gtgatgccgg agtcgttccg cttccccgac gcctcgatcg aggtctgggc
     6601 tccggaatcg ttgatcgagg cttcggcagc gccgcggagg cgcgacaacc ggttccagcg
     6661 ggtcatcgcc cggctcgatc gcggcgtgag cgtcgagcgg gcacggctcg agatgaacct
     6721 catcgcctca cggctggcgc aggagttccc cgagaccaat accggccggg acacggtcac
     6781 gctcgtatcg ctgcacgagt cgctcgtcgg gccacggctg cgcgcggcgg tcctcgtgct
     6841 gctggccggg gtgggcctgg tgctgctcat cgcctgcgcc aacgtcgccc acctgctcat
     6901 cgcgcgcatc gcctcgcgcc gtcgcgaaac ggcgatccgc ctcgccctcg gcgcgactcc
     6961 ctccggcatc gtgcgcctgc acgtcgccga gagcctcgtc gtgtcgcttg ccgggggatt
     7021 gctcgggctc ctgacggcgg cctggaccct ccagctggtg cggggcgggg tcctgccgta
     7081 cctgccggcc cagatggacg tcgtcctcga ccgtcgcgtc ctcgggttcg cgcttttgac
     7141 ctcgatcgcg gcggctctcc tgtgcggcgt gctgccggcg ctacgcggcg accggagcgt
     7201 cccgcaccag gacctgaagg aaggagcgcg cggggccggc gccggcgggg agcgccggcg
     7261 gctgcgcgag atcctggtca tcagcgaaat ggcgctcgcc gtcgtgctgg tggtggccgc
     7321 ggggctggcg ctcaagagct tccggcgcct gagcggcgtc gatcccggct tcgatccgga
     7381 cgcggtgctg accgtggcga tgaacgcctc cggggcgcgc taccccgagc gggcgcagtt
     7441 cgtcgagttc cagcgccggg tcgtcgaacg cgtgcagcac attccggggg tggtcgcagc
     7501 cggctcaacg cgcaacctgc cgctcctcgg gacgggggag tcctggccgg tcgtccccga
     7561 ggggggcgcc ccggtcacgc caggaaacga gccggaggtg gcggtccacc cggtcagcgc
     7621 cggcttcttc gccacgctcc ggatcccgct cctcgagggg cgcccgttca ccgagcccga
     7681 gagcggaccg ccggtcgcga tcgtcagccg ggcactggca cgccggttct ggccggacgg
     7741 gggcgccgtc ggcagcgcgc tcctggtcgg cgagacgcgc ctcgccgtcg tgggggtcgt
     7801 cggggacgtg cgacaggcga ccctggagca ggagggggag gcggccctgt acctgccgag
     7861 cgggctctcc cccaggcgcg gcttcacgct cgtgatccgc acgacggggc cgccgcgcgc
     7921 gctcgagtcc ctggtacgct ccgccatcca cgagatcgat ccgcagcagc cgatcctgcg
     7981 catcgccgcg atgcgcgagg tgctcgggag cgccctggcg caaccgcggc tgatgtcgct
     8041 cctgctcggc gccttcggga cgctcgcctt cgccctcgcg cttctcggcg tgtacggcgt
     8101 catcgcgctg atggtgtcgc tgcgcaccta cgagttcggc gtccggatgg cgctcggcgc
     8161 caccgccggc tccgtccgca ggatggtgct gcggcgggcg atcctgctgg gtgcctgcgg
     8221 catcggggcc ggcagcctcg ccggcctggg cctgacccgc ctcctgcgat cgcagctgta
     8281 cgccgtgagc ccggccgacc cgccgacatt cctcctcgcc gcggccgcgc tcctcgtggc
     8341 gtgcctgttc gccggcgacg gtccggcccg ccgcgccacc cgcatcgacc cgctcgtcgc
     8401 attgcgatcc gaataattgg ctccggcgct atactccgcg gcacagcctt cgccatcaaa
     8461 ccgaacagac cgcttcgaat gaggagggac tcatgaagaa gccgcgaccg acgtcccgct
     8521 tgcgccgtgc cgtccccgcc accgtcctgt tctgcgcgtc gctcgccgct cccggtgggg
     8581 cgcacgccga ccggaccgac atccagggca gcgttcctgt cctcgtgaag agccgggggc
     8641 cgctgaccgc cgagaccgcc gcggcgatcg gatcgcacgg cacgcacatc acctacctct
     8701 ggccgcagat cgacgcgatg gcgatgacgg tgaacccgtc ccggctggag gagctggctt
     8761 ccgacccggc cgtcgccttc gtcgagctgg accagcaggg aagcgcaccc ggggccggcc
     8821 cgtccggttc cggcgcgacg gcgccgatga cggccgtgcc gcttccccag gcagacgctc
     8881 cgatcgtccc ctggaacctc gacatggcgg atgtcttcgg caccggttac gacggggcgg
     8941 gcgtgaccgt ggcggtggtc gattccggtc tgccccagaa ctgggaagag tttctccccg
     9001 aaggggtcgt cgacacggag cacgcggctg gattcggggc ggagggatgg ggcgatttcc
     9061 acgcgccgct gcacgccatc cggggcgcgg gcggccacat cggcctcttc ccgcacggcc
     9121 tcgccgtcag cagcatcatc gccgggtttc cctccgaggc ggggcctgtc ggcggggccg
     9181 ccccgggcgc caggatcctc ccggtgcgcg tgctgaacca gttcaacttc ggctggttct
     9241 cctggttcgc ggcc
//
LOCUS       DASRIQ010000480         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_432879, whole genome shotgun sequence.
ACCESSION   DASRIQ010000480 DASRIQ010000000
VERSION     DASRIQ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432879"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="VFD06_11735"
     CDS             <1..1647
                     /locus_tag="VFD06_11735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN04243.1"
                     /translation="PEHPPLIKELAALPLLPLALDFPDGSLWQKAEEWNIGRIFVHEN
                     RLPNDTILFLGRLPVLVLSVLLGWILFRVGRRLFGPAGALLALALWVLDPNVVTHSGL
                     VTTDLGITLFIFLAALVLHAWIARPTRRGLVLLGLATGGAFAAKYTALWLPPILAGVS
                     LIAFLRDPGPAAIWSRPRWRRLLSLAAAAVPVVGIAFLVIAMCYGFVGLPAYLEGLDR
                     TLHHSATGHRAYLMGEITDTGWWYYFPLAFLWKTPPGTLLVLGAAVVAAALGARRAAR
                     DEIFLLVPIAITLAIVCAWKVNIGLRHILVIYPFLFLAAGRLLTPKRAGTRPPRWAMV
                     VAGLGVAWNGVEAARIAPYHLTYFNLIAGGPAEGHRYLLDSNYDWGQASKALKRYLDA
                     RGIPMVYCAFTGNSDPWYYGIRYQYAPGSGNLRNAKERPARVPMNAPRELLAINAMVL
                     HSLHFSDHHLYDWLLAREPVARPGHAWFVYDITRDADAHTYLGALCLSFGLLDLAEDQ
                     ANRALSFSPGHPLAQAVLEKARERRANPEAAPAPGSLRRP"
     gene            complement(1538..>2366)
                     /locus_tag="VFD06_11740"
     CDS             complement(1538..>2366)
                     /locus_tag="VFD06_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04244.1"
                     /translation="VRAQFNYGAASERARDDAGAEQAYRRALAVWPRFSDARYNLAGL
                     YGRAGHWREAAEEYRAALTEQPGNVTYLVNAGHAAAQAGDPRAAIELLERAVDLDRSS
                     GPGWTDLGAARLQIGDAAGALAAFREADRLAPGRPEAAANIALALEAAGQAPEAAAAW
                     RAAADGKPGDPLLRYRLGRALESAGRPDEAIEAYRDSIRLAPDTPVPLKALGLLLASR
                     GRNDEARAALERARRLDPSGRVMDDAAIRVLERLPGSPGARVPSPAPPAPGDAPGRN"
BASE COUNT          270 a          913 c          822 g          361 t
ORIGIN      
        1 ccggagcacc cgcccctcat caaggagctg gcggcgctgc cgctgctgcc tctcgcgctc
       61 gactttcccg acggatcgct ctggcagaag gcggaggagt ggaacatcgg ccggatcttc
      121 gtgcacgaga accgcctgcc gaacgacacc atcctcttcc tcggccggct gccggtcctc
      181 gtcctctcgg tccttctcgg gtggatcctc ttccgggtcg gccgccggct cttcggcccg
      241 gcgggggcgc tcctcgcgct cgccctctgg gtcctggatc cgaacgtcgt gacccactcg
      301 gggctcgtca cgaccgacct cggcatcacc ctcttcatct tcctggcagc gctggtgctc
      361 cacgcctgga tcgcccggcc gacccggcgg ggtctcgtcc tccttggcct ggccaccggc
      421 ggcgccttcg cggcgaagta cacggcgctg tggctgccgc cgatcctcgc gggggtgtcc
      481 ctgatcgcgt tcctgcgcga tcccggtccg gccgcgatct ggagccgccc gcggtggcgc
      541 cgcctgctca gcctcgcggc ggccgcggtg ccggtcgtcg ggatcgcctt cctcgtcatc
      601 gccatgtgct acggcttcgt cggtctgccg gcgtacctcg aggggctcga ccggacgctg
      661 caccattccg ccaccggcca ccgcgcgtac ctcatggggg agatcaccga caccggctgg
      721 tggtactact tcccgctcgc cttcctctgg aagacgccgc ccggaacgct gctcgtcctc
      781 ggcgccgccg tcgtcgcggc ggcgctcggg gcccggcgtg cggcgcgcga cgagatcttc
      841 ctcctcgtcc ccatcgccat cacgctcgcc atcgtgtgcg cctggaaagt gaacatcggc
      901 ctgcggcaca tcctggtgat ctacccgttc ctgttcctcg ccgccgggcg cctgctgacg
      961 cccaagcgcg cgggaacgcg gccgccgcgc tgggcgatgg tcgtcgccgg cctgggggtc
     1021 gcgtggaacg gcgtcgaggc ggcgcgcatc gccccctatc acctcaccta cttcaacctg
     1081 atcgccgggg ggccggcgga ggggcaccgg tacctgctcg attccaacta cgactggggg
     1141 caggcctcga aggcgctgaa gcgctacctg gacgcgcggg ggatcccgat ggtctactgc
     1201 gcctttaccg gcaactcgga cccctggtac tacggcatcc gctaccagta cgcccccggc
     1261 tcggggaacc tgcgcaacgc caaggagcgc ccggcgcggg tgccgatgaa cgcgccgcgc
     1321 gagctgctcg ccatcaacgc gatggtgctc cactcgctgc acttcagcga tcaccacctg
     1381 tacgactggc tcctggcgcg ggagccggtc gcgcgccccg gccatgcgtg gttcgtctac
     1441 gacatcacgc gggacgccga cgcgcacacc tatctcgggg cgctgtgcct gtcgttcggc
     1501 ctgctcgatc tcgcggagga tcaggcgaac cgggcgctca gtttctcccc ggggcatccc
     1561 ctggcgcagg cggtgctgga gaaggcacgc gagcgccggg cgaacccgga agccgctcca
     1621 gcacccggat cgctgcgtcg tccatgaccc ggccggacgg atcgaggcgg cgcgcccgct
     1681 ccagggcggc gcgcgcttcg tcgttgcgcc cgcgggaggc gaggagcagc ccgagcgcct
     1741 tgagcggcac cggcgtgtcg ggggcgaggc ggatcgagtc gcggtacgcc tcgatcgcct
     1801 cgtccggccg gccggcggac tccagcgccc gtccgaggcg atagcgcaga agcggatcgc
     1861 cgggcttgcc gtccgccgcg gcgcgccacg cggccgctgc ttccggcgcc tgcccggccg
     1921 cctcgagcgc cagggcgatg ttggcggccg cctccggccg gcccggggcg aggcggtccg
     1981 cttcgcggaa ggcggcgagc gcccccgccg cgtcgccgat ctgcaggcgc gcggcgccga
     2041 gatcggtcca tcccggcccg ctcgaccgat cgagatcgac cgcccgctcc aggagctcga
     2101 tggcggcgcg cgggtccccc gcctgcgccg ccgcgtggcc ggcgttgacg aggtaggtga
     2161 cgttccccgg ctgctcggtg agcgcggccc ggtactcttc cgccgcctcc cgccagtgcc
     2221 cggcccgacc gtagagcccg gccaggttgt agcgcgcgtc cgagaagcgc ggccagacgg
     2281 cgagggcacg gcggtacgcc tgttccgcgc ccgcgtcgtc gcgcgcccgc tccgaggcgg
     2341 cgccgtagtt gaactgcgcc cgcacg
//
LOCUS       DASRIQ010000481         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1752665, whole genome shotgun sequence.
ACCESSION   DASRIQ010000481 DASRIQ010000000
VERSION     DASRIQ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752665"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFD06_11745"
     CDS             complement(<1..397)
                     /locus_tag="VFD06_11745"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HZN04245.1"
                     /translation="MSTAAIVGLILLSILVGALIPVLYQAWLTLKSARRQIDALGPRV
                     DQALKEITETTGRVNRIASIVEEHGERLGPVVDSAVEIGHTLGQVKNTIRKFSSLVGA
                     FGPAVVAGLGAFLAHRRARSATATTTEEET"
     gene            complement(410..2062)
                     /gene="nadB"
                     /locus_tag="VFD06_11750"
     CDS             complement(410..2062)
                     /gene="nadB"
                     /locus_tag="VFD06_11750"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZN04246.1"
                     /translation="MAQADWDLIVVGSGIAGLRAAIAVAAAGRRAALLTKDGATDSST
                     DKAQGGIAAVLSEDDELALHYEDTLVAGDGLCNEAAVRVLVEEGPTRALELIEWGARF
                     DREGTRLALAREAAHSKRRILRAQGDSTGREIVRALVEKTATLDGIALLPRTFSVDLV
                     MQGGRIAGLLALDEERETVRLLRAPAVLLATGGAGLIYSETTNPPQATGDGAAMAYRA
                     GAEMMDLEFVQFHPTALFLPGAPRFLLSEAMRGEGGRLVDAAGRPFMTEFDPRGDLAP
                     RDIVARGIHAVMRRSGAPCVHLDMTALPAEAVRARFPRIAATCARYGLDIARDHLPVA
                     PCAHFMMGGVKTDLMGRTAIPGLYAAGEVACTGVHGANRLASNSLLEGLVFGARAGTA
                     VIQDRILEGGLAPEGPAFAASAAQALAEAVRSIDPREAEGIVARLRRVMWDDVGLVRD
                     AEGMRRAVRTILNLESGLSPRPPARAGLEARNLLFVARLVAESALRREESRGSHYRSD
                     HPRPEPGRGRHAVIAPGAPAPDLPTLERPITASAPASARVVD"
     gene            2176..>2617
                     /locus_tag="VFD06_11755"
     CDS             2176..>2617
                     /locus_tag="VFD06_11755"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZN04247.1"
                     /translation="MPDAPVATPLAGVFPPRLVARLHRQRFEGTLRLHGGGATRVIYF
                     RRGEIASAASNADEDRLPNILIREGRLTGPQVEMARAKQRPGVSLGKTLIELGFLTPT
                     ELLQGARRQVRQILAAAFALREGTSQEAPGPLPPEVTVLGLPVRR"
BASE COUNT          357 a          992 c          907 g          361 t
ORIGIN      
        1 ccgtttcttc ttccgtcgtg gtcgcagttg cggaccgggc ccgccgatgt gcgaggaacg
       61 cgcccaggcc cgcgacgacg gccggcccga aagcgccgac cagactgctg aacttgcgta
      121 tggtgttctt gacctgacca agggtgtggc cgatctcgac cgccgagtcg accacggggc
      181 ccaggcgttc cccgtgctct tcgacgatgc tggcgatgcg gttgacccgc cctgtcgttt
      241 ccgtgatttc cttcaacgcc tgatcgaccc gtggcccgag cgcatcgatc tggcgccgcg
      301 cgctcttcaa cgtcagccac gcctgataga ggaccgggat cagggccccg acgaggatgc
      361 tgaggaggat gagccctacg attgccgcag tggacatgag gcactctcct tagtcaacga
      421 ctctagcaga cgccggggcc gaagctgtaa tcggacgctc gagcgtcgga aggtcgggag
      481 cgggcgcccc cggggcgatg acggcgtggc gcccccggcc cggctcgggt cgggggtgat
      541 cggatcggta gtggctgccg cggctctcct cgcggcgcag cgcgctctcg gccacgaggc
      601 gcgccacgaa caatagatta cgcgcctcga gccccgcgcg cgccggcgga cgtggggaga
      661 gccccgactc gagattcagg atcgttctga cggcccggcg catcccttcc gcgtcgcgca
      721 cgaggccgac atcgtcccac atcacccggc gcagccgcgc gacgatcccc tccgcttcgc
      781 gcggatcgat cgaccggacc gcctccgcca aagcctgcgc cgcgctggcg gcaaacgccg
      841 gaccttccgg agccaggccc ccttcgagga tccggtcctg gatcacggcg gtcccggcgc
      901 gcgccccgaa gacgagcccc tcgagcagcg agttgctcgc gagccggttg gcgccgtgca
      961 caccggtgca ggcgacctct cccgccgcgt acagccccgg gatcgccgtc cgccccatca
     1021 ggtcggtctt caccccgccc atcatgaagt gggcgcacgg cgcgaccggc aggtggtcgc
     1081 gggcgatgtc gagaccgtaa cgggcgcagg tcgcggcgat gcgcgggaag cgcgcccgca
     1141 cggcctcggc cggaagcgcc gtcatgtcga ggtggacgca cggcgcgccg ctccgccgca
     1201 tcactgcgtg gatgccgcgg gcgacgatgt cgcgcggcgc gagatcgccg cgcgggtcga
     1261 actccgtcat gaacggccgc ccggcggcgt ccacgaggcg tcccccctcg ccgcgcatcg
     1321 cctccgacag gaggaatcgc ggcgcaccgg gcaggaagag cgcggtcgga tggaactgga
     1381 cgaactccag gtccatcatc tcggccccgg cgcggtaggc catcgcggcg ccgtccccgg
     1441 tcgcctgcgg cgggttggtg gtctcgctgt agatcagtcc cgctccgccg gtcgcgagca
     1501 ggaccgccgg cgcccgcagc agccgcacgg tctcccgctc ctcgtcgagg gcgaggagcc
     1561 cggcgatccg tcccccctgc atcacgagat cgacgctgaa agtgcgcggc aggagggcga
     1621 tgccgtcgag cgtcgcggtc ttctccacga gcgcccggac gatctcacgc ccggtggaat
     1681 ccccctgcgc acggaggatg cgccgcttgc tgtgcgccgc ctcgcgcgcc agcgccaggc
     1741 gcgtcccctc gcgatcgaag cgggcccccc actcgatgag ctcgagggcg cgtgtcggac
     1801 cttcctccac gaggacccgg accgccgctt cgttgcacag gccgtcgccg gcgacgagcg
     1861 tgtcctcgta gtgcagcgcc agctcgtcgt cctcggacag gacggcggcg atccccccct
     1921 gggccttgtc ggtggatgaa tcggtggcgc cgtccttggt gaggagcgcg gcgcggcgcc
     1981 ccgcagccgc cacggcgatg gcggcgcgca gcccggcgat gccgctgccg acgacgatca
     2041 gatcccagtc cgcctgcgcc acgatcgcct ccccgggccc ctcgacggca gtctacccgt
     2101 ttgacagggg gtgcgccgct ccctatgatg cggcccttcg cgccccgggc gcgaaggagg
     2161 tcgcctccac ccgccatgcc ggacgctcca gtcgcgacgc cgctcgccgg cgtctttccg
     2221 ccgcgcctcg tcgcgcgcct ccatcgccaa cgcttcgagg gaacgctgcg cctgcacggc
     2281 ggcggcgcca cccgggtcat ctacttccgc cgaggcgaga tcgcctccgc cgccagcaac
     2341 gccgacgagg accgcctccc gaacatcctg atccgcgagg ggcgcctgac cggaccccag
     2401 gtggagatgg cgcgggcgaa gcagcgcccc ggcgtgtcgc tcggcaagac cctcatcgag
     2461 ctcggcttcc tgacgccgac cgagctgctg cagggggcgc ggcgccaggt gcggcagatc
     2521 ctcgccgccg ccttcgcgct gcgcgagggg acgagccagg aagcccccgg gccgctccct
     2581 cccgaagtga cggtgctcgg ccttccggtg cggcgcc
//
LOCUS       DASRIQ010000482         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_205348, whole genome shotgun sequence.
ACCESSION   DASRIQ010000482 DASRIQ010000000
VERSION     DASRIQ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205348"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFD06_11760"
     CDS             <1..878
                     /locus_tag="VFD06_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN04248.1"
                     /translation="GKELVARAIHDRSNRGARRFFSENCSALSESLLESELFGHVRGA
                     FTGADRDRKGILELAHGGVLFLDEIGDMSLRMQSKLLRALQEREIRPVGGKETIRIDV
                     RLISATHRSLPLLIRDGGFREDLFYRVNVITIHLPPLRERRDDIPSLVDHFLGRIASE
                     TGLPRKQCSSGALELLVAYDWPGNIRELENIVQRAVALGDGRRIEAGDLPDRIRHVMI
                     TEEAPHYSGAGKAGEQLLIEKALFNYDGDKTRAARFIGWSRPKLYRRMHAYGIPRDFG
                     RHRDRAMAILRPHDA"
     gene            complement(920..2647)
                     /locus_tag="VFD06_11765"
     CDS             complement(920..2647)
                     /locus_tag="VFD06_11765"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HZN04249.1"
                     /translation="MPSRSAPAPAPQILEEIAFQKATVARLRREIGDVDRTSKRGYIR
                     EFHSSIGRFQAAASWDDLVIACYRADLVYIGDYHALPSAQETAARLLQAIAERSRAVV
                     LGVEMVYGRHQRVLDRYMSGELDESAFLRAIRYDSDWGYEWSSFQRIFEVARGQAVPV
                     IGLDCPPRTGFRHIRRRDRYAADRIAGMIAAAPDARAVVLFGESHLAPSHLPRQVTAR
                     LKRKGIEKRVVTVVQNLEEIYWQMSEAGHPQSEVARLGEASYCHFSAGPIAKYEAYRR
                     TIELWKAEEGEDDPVDLTSTVHGIIDMILRFLKIDPYTRHLKLPGRGRDLIVDLYPDI
                     HSGLEADEVRILFKSSRLPEEDAAEVLDHLTRSGSCYVPRLNAIFLGEFSLQHAGEEA
                     AHFINQLLKGEIYEAMPRALPQHDLFYGAVLEEALGFFGSKIVDPSRNHFFETEFYQY
                     YRKDKETIETNTRYTFDEFNEIIEFVLLHKKFEHSYEHYGEVPEAILAGIRADPHRVN
                     VLVHELGYFLGQQLHDGYRMGLVDRRDMLALFHASFRRSGSALAAYLDLSRKVAPALQ
                     GGLTPAGAG"
     gene            complement(2672..2842)
                     /locus_tag="VFD06_11770"
     CDS             complement(2672..2842)
                     /locus_tag="VFD06_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZN04250.1"
                     /translation="MGSIGITELVLIFVIILLIFGGRKIPELAKGLGEGIRHFRDSLH
                     GQGPEEKKDKPS"
     gene            complement(2914..>4447)
                     /locus_tag="VFD06_11775"
     CDS             complement(2914..>4447)
                     /locus_tag="VFD06_11775"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HZN04251.1"
                     /translation="AKTGEGLDVLRRALSDAARAIPPRDAAGLARLPIDRAFTMKGFG
                     AVVTGTLVAGTLAEGQEVEILPERRAARIRGLQVHGRATPRAGAGQRLAVNLQGIEVA
                     ALERGQTLTERGVLKPTRVLDVALEHLPGGTPLKDQARLSLHMMTAEVACRVRLLEGP
                     ALPPGARAYAQLRCARSVTAILGDRFILRRPSPALTIAGGTVLHNGPRAGRLRPADRL
                     RRCKGLDDPSTLTALLALVEDAGDEGVDAASLRSRTGLDAAAVAARLESKEGIDAAMA
                     IPSNPRRYVGRAAVERLSREVLDAARDFHRREPLREGLAREEVRTRLFRRSHDDVFRA
                     VIADLVRRNQLRADKDRIALATHQVSLAPAEAALLERLEQSYLSGGSNPPEKDEAAKG
                     VGADPRTADRLIHLLLSRGRLVRIPDGKIFHAEAIQALKERLWERRTKGESIDIGEFK
                     DLSGTSRKNAIPLLEYFDQTQLTRRQGNLRIILPPPAHPLPPPARALPPPAPRAGKPR
                     GD"
BASE COUNT          648 a         1548 c         1593 g          658 t
ORIGIN      
        1 ccggcaaaga gctggtggca cgcgccatcc acgatcgctc caaccgcggc gcgcgccgct
       61 tcttctccga gaactgctcc gccctctccg agagcctgct cgaatcggag ctgttcggcc
      121 acgtgcgcgg cgccttcacc ggcgccgacc gcgaccgcaa agggatcctc gagctggcgc
      181 acggcggcgt gctcttcctc gacgagatcg gcgacatgag cctgcgcatg cagagcaagc
      241 tgctccgggc gctgcaggaa cgggagatcc ggccggtcgg aggcaaggag acgatccgga
      301 tcgacgtgcg gctgatctcc gccacgcacc gcagcctgcc tctgctcatc cgcgacggcg
      361 ggtttcgcga agacctgttc taccgcgtca acgtgatcac catccacctg ccgccgctcc
      421 gggagcggcg cgacgacatc ccgtcgctcg tcgatcactt cctcgggcgc atcgcctccg
      481 agacggggct gccccgcaag cagtgctcct ccggggcgct ggagctgctc gtcgcgtacg
      541 actggcccgg caacatccgc gagctggaga acatcgtgca gcgcgccgtc gcgctcggcg
      601 acgggcgccg catcgaggcg ggcgacctgc cggaccggat ccggcacgtg atgatcaccg
      661 aggaggcccc gcactacagc ggcgccggca aggccggcga gcagctcctg atcgagaagg
      721 cgctcttcaa ctatgacggc gacaagacgc gcgccgcccg cttcatcggc tggagccgcc
      781 ccaagctgta ccggcgcatg cacgcctacg gcatcccgcg cgacttcggc cgccaccgcg
      841 atcgggcgat ggcgatcctg cggccccacg atgcgtgacg cgcggcgacc tgagcgatgt
      901 gacgaggagg tcggtcggat caaccggccc ccgccggtgt gagccccccc tggagcgccg
      961 gcgccacctt gcgcgacaga tcgaggtagg cggcgagcgc gctgccggag cggcggaacg
     1021 aggcgtggaa gagggccagc atgtcgcgcc ggtccaccag ccccatgcgg tagccatcgt
     1081 gcagctgctg cccgaggaaa taccccagct cgtgcaccag cacgttgacg cggtgcgggt
     1141 cggcgcggat gccggcgagg atcgcctccg gcacctcgcc gtagtgctcg taggagtgct
     1201 cgaacttctt gtgcagcagc acgaactcga tgatctcgtt gaactcatcg aacgtgtacc
     1261 gcgtgttcgt ctcgatcgtc tccttgtcct tgcggtagta ctggtagaac tcggtctcga
     1321 agaagtggtt tcgggacggg tcgacgatct tcgatccgaa gaagccgagc gcctcctcga
     1381 ggacggcgcc gtagaagagg tcgtgctgcg gcagggcgcg cggcatcgcc tcgtagatct
     1441 cccccttcag gagctggttg atgaagtggg ccgcctcctc tccggcgtgc tgcagcgaga
     1501 actcgccgag gaagatggcg ttgagccgcg gcacgtagca ggagccgctg cgggtgaggt
     1561 gatcgagcac ctccgccgcg tcctcctccg gcaggcggga gctcttgaac aggatgcgca
     1621 cctcgtcggc ttcgaggccg gagtggatgt ccggatagag atcgacgatg aggtcgcgcc
     1681 cgcgtcccgg cagcttgagg tggcgcgtgt acgggtcgat cttcaggaag cgcaggatca
     1741 tgtcgatgat cccgtgcacc gtggaggtga ggtccaccgg gtcgtcctcc ccctcctccg
     1801 ctttccacag ctcgatggtg cggcggtacg cctcgtactt ggcgatcggt ccggcgctga
     1861 agtggcagta cgacgcctct cccaggcgcg cgacctcgga ctgagggtgg ccggcctcgg
     1921 acatctgcca gtagatctcc tcgaggttct gcaccacggt caccacccgc ttctcgatcc
     1981 ccttccgctt caggcgggcg gtcacctggc gcggcaggtg cgacggggcg aggtgcgact
     2041 cgccgaacag gacgacggcg cgcgcgtcgg gcgcggcggc gatcatcccg gcgatccggt
     2101 cggcggcgta gcggtcgcgg cggcggatgt ggcggaagcc ggtgcgcggc gggcagtcga
     2161 gcccgatgac cgggactgcc tgcccgcgcg ccacctcaaa gatccgctgg aacgacgacc
     2221 actcgtaccc ccagtcgctg tcgtagcgga tggcgcgcag gaacgccgac tcgtcgagct
     2281 cgccggacat gtaacggtcg aggacgcgct ggtgccggcc gtacaccatc tcgacgccga
     2341 gcacgaccgc ccgcgaccgc tcggcgatcg cctgcagcag ccgcgccgcc gtctcctgcg
     2401 cgctcggcag ggcgtggtag tcgccgatgt acacgaggtc ggcccggtag caggcgatga
     2461 ccaggtcgtc ccacgaggcc gcggcctgga agcgaccgat gctggagtgg aactcgcgga
     2521 tgtagccgcg cttgctggtg cgatcgacgt cgccgatctc ccggcggagt cgggcgacgg
     2581 tggccttctg gaaggcgatc tcttccagga tttgcggcgc cggggcaggc gcgctgcgcg
     2641 acggcatggg cgacgcgacg ggggggaggc gctacgaggg cttgtccttc ttctcttccg
     2701 gcccctgtcc gtggagtgaa tcacggaagt ggcggatgcc ctcaccgagt cccttcgcca
     2761 gctcggggat cttccgcccg ccgaagatca gcaggatgat gacgaagatc aggacaagtt
     2821 cagtaatgcc gattgaaccc atgaggtcac ctcggtggca tcttagacgc gacccccggg
     2881 ggtgtcaatc gcacggactc gccccgtcgg gcgtcaatcc ccgcgcggct tcccggcccg
     2941 aggggccggc ggcgggagcg cgcgggcggg cgggggaagg gggtgggcgg gggggggaag
     3001 gatgatgcgc aggttcccct gccgccgcgt cagctgagtc tggtcgaagt actcgaggag
     3061 cgggatggcg ttcttgcggc tggtgccgct caggtccttg aactcgccga tgtcgatcga
     3121 ctcccccttc gtgcgccgct cccacaggcg ctccttcagc gcctggatcg cctcggcatg
     3181 gaagatcttg ccgtccggga tgcgcaccag ccggccacgc gacagcagca gatggatcag
     3241 ccggtcggcg gtgcgggggt cggcgccaac ccccttggcc gcttcgtcct tctcgggggg
     3301 gttggagccg ccggagaggt aggactgctc caggcgctcg aggagcgccg cctcggcggg
     3361 ggcgagcgac acctggtggg tggcgagggc gatccggtcc ttgtcggcgc gcagctggtt
     3421 ccggcgcacg agatcggcga tcaccgctcg gaagacgtcg tcgtggctgc gccggaagag
     3481 ccgcgtccgc acctcttcac gggcgagccc ttcgcggagc ggctcgcgcc tgtggaagtc
     3541 gcgcgcggcg tcgagcacct cgcgcgacag ccgctcgacg gcggcacggc cgacatagcg
     3601 ccgcgggttc gacgggatcg ccatcgccgc gtcgatcccc tccttcgact ccagccgggc
     3661 ggcgaccgcc gccgcatcga ggccggtgcg gctgcggagc gaggcggcat ccaccccttc
     3721 gtcccccgcg tcctcgacca gcgcgagaag cgccgtgagc gtcgacggat cgtcgaggcc
     3781 tttgcagcgg cgcaggcgat cggccggacg caggcgcccg gcgcgcggcc cgttgtggag
     3841 caccgttccc cccgcgatcg tcagcgccgg cgagggacgc ctgaggatga accggtcgcc
     3901 gaggatcgcc gtcacagacc gggcgcagcg caactgcgcg taggcgcgcg ccccgggcgg
     3961 aagggccggg ccctcgagca ggcgcacacg gcacgcgacc tccgcggtca tcatgtgcag
     4021 cgacagacgc gcctggtcct tgagcggcgt cccgccgggg aggtgctcga gcgccacgtc
     4081 gagcacccgg gtcggcttca gaacaccccg ctcggtgagc gtctgaccgc gctcgagcgc
     4141 cgcgacctcg atcccctgca agttgacggc gaggcgctgt ccggcccccg cccggggtgt
     4201 cgcgcgcccg tgcacctgga gtccgcggat gcgcgcggcg cgccgctccg gcaggatctc
     4261 cacctcctgc ccctccgcga gcgttccggc gacgagcgtc ccggtcacga ccgcgccgaa
     4321 ccccttcatg gtgaaggcgc gatcgatcgg caggcgcgcc agcccggcgg cgtcgcgcgg
     4381 cgggatggcg cgcgccgcgt cggacagcgc ccgccgcagc acgtcgagcc cttcgccggt
     4441 cttggcg
//
LOCUS       DASRIQ010000483         4909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2521920, whole genome shotgun sequence.
ACCESSION   DASRIQ010000483 DASRIQ010000000
VERSION     DASRIQ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2521920"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..4569)
                     /locus_tag="VFD06_11780"
     CDS             complement(46..4569)
                     /locus_tag="VFD06_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04252.1"
                     /translation="MRRLRAICPILLLVTCLAWGAQPASDAPVIAMTQTMRLATFFVG
                     AAVLAAGDPASVNGPAAGADPRPAEARILELTSPQRVESNYHGESSRVATLRQGKMQA
                     RAMASADFDEDGMPDLLVAEAAPDGTGAIAVHRGNVDALYPNQPEARARRDAGRFLAA
                     PFLPDVAVFDAADACDLIGAGDFDADGHQDAVTARRGGKALWWHRGDGKRGLGAPKTI
                     ELPGTVTALAVGEINRTDGLEDVVVAVDTSRGAETLVFEGPEGALRASPEVIPLSGSV
                     SGMALGALDDRPGRDLIVASGSTLQLISGRDRRLSLDEADRAAVPPAVLRTMEMPFII
                     QALAVGKFDDEPGADIALLDAARRLSVIPARAIAEEAAEPLEKMSPVSIPGLEAAPEP
                     GGARLMSARVSSRPGDDLLLFDGASSGVLTRTAAGEAGERRFALARTLGAVVDGLAMR
                     LGPDALDDLVLAGEGPAPSVSPSGPLTIRVVTNTSDAGPGSLRQAILDTNAAAGLDSI
                     TFNIPGPGPHTIAPKTPLPSVTDPVLLDGTSEPDFAGLPIVRLDGLIDASLPLIRVIG
                     DASTVRGLLFTRHDGPHAALEFWPGSDHIVEGNFFGTDPTGTIALPNGAALTVGSDDT
                     RIGGTVAAARNLIAGNDIGITAYGANVQIMGNYIGIDATGSAWLPNESGVQLRGPDET
                     VGGTTAGSRNLIVGGFFWGVGCFADAGGDLVQGNYFGTNAAGTAIVGAGTDAITMRNT
                     GGHTIGGTTPAARNVVGGHQSGITVLDLVAPSANNQIQGNYVGVGADGSTAIPNETGI
                     RIESGPLNIIGAIAGGGRNVISGNEDGIHLVHGPGGSRVYGNYIGTNATGTSAVPNIN
                     GIEIRTPDNHIGGASLAAANVISGNRSGVEVIDGTSNLIEMNLIGTDPSGTTLIPGEM
                     GLHGILLLGASGNMVGVKQGQGNVIAGFSSGIHMQDADLNLIQGNKIGTDITGAVALG
                     NDTGIHVNLSSENRIGGVADGVGNLISGNDTGIWLFNGENNEIQGNQIGTNLAGAAAL
                     PNRVGLLVQARNNFIGNATLERRNVISGNLEEGIALHTYGTSIAGNFIGTTADGLGPL
                     GNGSSGLRIAAVTAPPAGDFNEIGRASAPEGGNVIAFNGGAGIEVESGAHQRMSRNSL
                     YGNAGLGIDLGGDLVTPNDPGDVDTGPNQRQNFPVLTTVTTTGGSVAVGGTLDSTPLT
                     SFGLEFFASPSCDPLGYGEGRTYLGASSSASDPSGHLSFLASFIGTVGTGEAITATAT
                     SGAGDTSEFSACLAAVGGPPAAVTGLKLTTGPTEGLSWDATPGATLYHLYVGTRATLP
                     GLATQAADSCTVGAFPATSVFFPPAANPPPGEMLWALVTAEGKYGEGPVQPGSNGPRQ
                     LTSVGTCGDSCAHDMCSTGGPLQQACTFQAAFVCAKDPSCCDPVNGTWDFQCVQQVRT
                     VGRSLLCPESQGQCPHTLCNAGQPLPPGCDTPPLPVSCTAAVCQADPHCCQQAWDDAC
                     IAKVASVCGYNCV"
BASE COUNT          690 a         1748 c         1688 g          783 t
ORIGIN      
        1 cgcgcccccc cgggcccgac gccgcgccga cctgaatcct tctggctaga cgcagttgta
       61 gccgcagacc gacgccacct tggcgatgca ggcgtcgtcc caggcctgct ggcagcagtg
      121 cgggtccgcc tggcacaccg ccgccgtgca cgacaccggc aaaggcggcg tgtcgcatcc
      181 gggcggcagc ggctgccccg cgttgcacag ggtgtgcggg cactgcccct ggctctccgg
      241 gcacaacagg ctccgcccca ccgtcctcac ctgctggacg cactggaagt cccacgtccc
      301 gttcaccggg tcgcagcagg acgggtcctt ggcgcacacg aacgccgcct ggaaggtgca
      361 cgcctgctgc agcggccctc ccgtgctgca catgtcgtgg gcgcaggaat cgccgcacgt
      421 tccgacgctc gtcagctgcc gcggcccgtt gctccccggc tgcaccggcc cctcgccgta
      481 cttcccctcg gcggtcacca gcgcccagag catctcccct ggcggcgggt tcgcggccgg
      541 cgggaagaag accgacgtgg cggggaacgc tccgaccgtg cacgaatcgg cggcctgcgt
      601 ggccagcccc ggaagcgtgg cccgcgtccc gacgtagagg tggtacagcg tcgcgcccgg
      661 ggtggcgtcc cacgacagtc cctccgtggg gccggtcgtg agcttcaacc cggtgacggc
      721 ggcaggcggg ccgccgacag ccgccaggca cgcggagaac tccgacgtgt ccccggcgcc
      781 gcttgtggcg gtcgcggtga tggcctctcc cgtccccacc gtcccgatga aggaggcgag
      841 gaagctcagg tgcccggatg ggtccgacgc gctggacgac gcaccgaggt aggtccgccc
      901 ttccccgtat ccgagcggat cgcacgaagg tgaggcgaag aactccagcc cgaacgatgt
      961 gaggggggtg ctgtcgaggg tgccgccgac agcgacgctc ccgccggtgg tggtgacggt
     1021 ggtgagaacc gggaagttct gccgctgatt cggcccggta tcgacgtccc cggggtcgtt
     1081 cggcgtgacg agatcgccgc cgagatcgat ccccagcccc gcgtttccgt agaggctgtt
     1141 cctcgacatc cgctggtgag cgcccgactc gacctcgatg ccagccccgc cgttgaaagc
     1201 aatcacgttc cccccctccg gagcggaggc gcgaccgatc tcattgaagt cgcccgcggg
     1261 cggggccgtg acggcggcaa tccgcaggcc gctggacccg ttgccgagcg gcccgagacc
     1321 gtccgcggtc gtgccgatga agttgccggc gatcgaggtg ccataggtgt gcagtgcgat
     1381 cccttcctcc agattgccgc tgatgacgtt gcggcgttcg agcgtcgcgt tcccgatgaa
     1441 gttgttccgc gcttgcacca ggaggccgac gcggttgggg agtgccgccg cgcccgcgag
     1501 gttcgtgccg atctgattcc cctggatctc gttgttctcc ccattgaaca gccagatccc
     1561 ggtgtcgttg ccggagatca ggtttccgac cccgtctgcg acgccgccga tccggttctc
     1621 cgaactcagg ttgacgtgga tgccggtgtc gttccccagc gcgaccgcgc cggtgatgtc
     1681 cgtgccgatc ttgttcccct ggatcaggtt gaggtcggcg tcctgcatgt ggatcccgga
     1741 gctgaacccc gcgatgacgt tcccctgccc ctgcttcaca ccgaccatgt tccccgaagc
     1801 gcccaggaga aggatgccgt ggaggcccat ctctcccgga atcagtgtcg tcccggaggg
     1861 atcggtgccg atcaagttca tctcgatcag gttgctggtg ccgtcgatga cctccacccc
     1921 ggatcggttc ccgctgatca cgttcgcggc cgcgagggac gctccgccga tgtggttgtc
     1981 cggagtccgg atctcgatac cgttgatgtt ggggaccgcc gacgtcccgg tcgcgttggt
     2041 gccgatgtag ttcccgtaga ctcgactgcc tcccggcccg tggaccagat ggattccatc
     2101 ctcgttgcca gagatgacgt tccgaccgcc cccggcgatc gctccgatga tgttgagcgg
     2161 tccgctttcg atgcggatcc cggtctcatt ggggatggcg gtcgacccgt ccgcgcccac
     2221 gccgacgtag ttcccctgga tctggttgtt cgccgaaggg gccacgagat ccaacaccgt
     2281 gatcccgctc tgatgccccc ccaccacgtt gcgcgccgca ggcgtggtcc cgccaatcgt
     2341 gtgcccgccc gtgttccgca tggtgatcgc gtccgtgccc gcgccgacga tcgccgttcc
     2401 cgcggcgttc gtcccgaagt agtttccctg gacgagatcg ccccccgcat cggcgaagca
     2461 gccgacgccc cagaaaaagc cgcccacgat gaggttgcgc gatccggctg tcgtccctcc
     2521 gaccgtttcg tcagggcccc ggagctgcac cccactctcg ttcggcagcc aggccgatcc
     2581 cgtcgcatcg atcccgatgt agttgcccat gatctgcacg ttggcgccgt aggcggtgat
     2641 cccgatgtcg ttccccgcga tgaggttccg cgccgcggcg acggtgccgc cgatccgggt
     2701 gtcgtcggaa cccacggtga gcgccgcgcc gttgggcagc gcgatcgtcc cggtcggatc
     2761 cgttccgaag aagttccctt cgacgatgtg gtccgacccg ggccagaact cgagggcggc
     2821 gtgggggccg tcgtgacgcg tgaagaggag cccgcggacc gtcgacgcat ccccaatgac
     2881 ccggatgagc ggcaagctcg catcgatgag gccgtcgagg cgcacgatcg gcaggccggc
     2941 gaaatccggc tcgctcgtac catcgagcag aaccggatcg gtcacggagg ggagcggcgt
     3001 cttcggggcg atcgtgtggg gaccgggccc ggggatgttg aacgtaatgg aatcgagccc
     3061 cgcggcggca ttggtgtcga ggatcgcctg gcggagcgag ccgggcccgg catcgctggt
     3121 gttggtgacc acgcgtatcg tcagcggtcc cgagggggag accgacggcg cagggccttc
     3181 ccccgccagg accagatcgt ccagcgcgtc ggggccgagg cgcatcgcga ggccgtccac
     3241 gaccgcgccg agcgtccgcg cgagcgcgaa ccgccgctcg ccggcctcgc ctgcggcggt
     3301 ccgcgtgagc actcccgagg aggcgccgtc gaaaaggagc aggtcgtccc ccggccgcga
     3361 cgagacccgc gcgctcatga gcctggctcc gccgggttcc ggcgccgctt ccagcccggg
     3421 gatcgacacc ggcgacatct tttcgagcgg ttccgccgcc tcctccgcga tcgcccgcgc
     3481 cgggatcacc gacagccggc gcgctgcgtc gaggagggcg atgtccgcgc ccggttcgtc
     3541 gtcgaacttc cccaccgcaa gcgcctggat gatgaagggc atctccatgg tgcggagcac
     3601 ggccggcggg acggcggcgc ggtccgcttc gtcgagcgac aggcggcgat cgcggccgga
     3661 aatcagctgc agcgtcgagc ccgaggccac gatcagatcg cgccccggtc gatcgtccag
     3721 cgcgcccagc gccatccccg acaccgatcc ggagagcgga atcacctcgg gagacgcgcg
     3781 cagcgccccc tcggggcctt cgaagacgag cgtctcggcc ccgcggctcg tatcgacggc
     3841 caccacgacg tcctcgagac cgtccgtccg gttgatctcg ccgaccgcga gcgccgtgac
     3901 cgttccgggg agttcgatcg tcttcggggc tccgaggccg cgcttgccgt ccccgcggtg
     3961 ccaccagagt gccttgccgc cccggcgggc cgtcaccgcg tcctggtgac catcggcatc
     4021 gaaatcgccc gcgccgatca ggtcgcacgc gtcggcggcg tcgaacaccg cgacgtccgg
     4081 caggaacggc gccgccagga agcggcccgc gtcacgccgc gccctcgctt cgggctgatt
     4141 cgggtagagg gcatcgacgt tgccccggtg aacggcgatg gctcccgtac cgtccggagc
     4201 cgcctccgcg accaggaggt cgggcatgcc gtcctcgtcg aagtcggcgc tggccatggc
     4261 gcgcgcctgc atcttcccct gccggagcgt cgcgacgcgc gaggattcgc cgtggtagtt
     4321 cgactccacc ctctgcgggg acgtgagttc gaggatgcgg gcctctgctg gacggggatc
     4381 cgcgcccgcc gccggtccgt tcacgctcgc ggggtcgccg gccgcgagca ccgcggcgcc
     4441 gacgaaaaac gtcgccaacc tcatggtctg ggtcatcgcg atgaccggag catcgctcgc
     4501 aggctgagct ccccaggcca gacaggtgac cagcagcagg atcggacaga tggctcgcag
     4561 tctcctcatc gacgcctcca gcgagagcgg gccggacggc tccggccccg ttgctctccc
     4621 tttgtagaat gagctgcggc ggtgttaccg atccggaggt cggaggacga tgacgaacga
     4681 gcggccggag gccgcggcga gattgtctgg ccacgacggc ctcaactccg cccttcgacc
     4741 ttgaccggga gctgggtccg ggcgtatacg gaccttgttc ccgacgatgg ggggtgtggg
     4801 aagagaggag gaggttatgc gcgtccacgt ggcgttcgcg gtgctcctga cggcggtgtc
     4861 ggtctggagc gccggcgcgg cctccgccgc ggctcggccc ccctcttcc
//
LOCUS       DASRIQ010000484         5220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2812329, whole genome shotgun sequence.
ACCESSION   DASRIQ010000484 DASRIQ010000000
VERSION     DASRIQ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2812329"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFD06_11785"
     CDS             <1..313
                     /locus_tag="VFD06_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN04253.1"
                     /translation="SAELLILSLVEARPRHGYEISRQIETRSGGALRFHVASLYPLLY
                     RLERRGWIAGRWVEKAGQRRRRYYRLTREGRKILTAQRSTWESFVAAIARITRPEPSE
                     G"
     gene            317..3010
                     /locus_tag="VFD06_11790"
     CDS             317..3010
                     /locus_tag="VFD06_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04254.1"
                     /translation="MPDWRDEIRRRLPPETRGVGRREGVVEELAQHLEDVHAALLAEG
                     VSPEEAERRTRAELHGSGAEDRLTAPGGAPPAPPLEALAKGSLLGDLRQDLAHGLRLL
                     RAQFGFAAIAILTLGLGTGAVAAIFSIVNAVLLRPLPFRDPERLVAVSLRRADDVRHP
                     FNLPDFLDDRDQNRTLEGIAAYGNWSGNMTGVGEPERLSGLRVSADLFELLGVRALAG
                     RTLLAGDDRPENRRVVVLGHSLWRRRFGGDPGVVGRTIVLNGDGYEVVGVLPAFFPFP
                     IPEAELAIPLAPDADPQRSVRSSVNFLRAVARLGPGVTREQAESDLNTLAQQQRREHP
                     VENAQKMGRTLTPLHEELVGGFGPALWILFGAVGLVLLIASVNLAGLALARAAARRKE
                     MAIRAALGATRRRLARQIAAECLLLALFGAAAGIVVAVFGVDLLLAMSPAGLPRAAEV
                     HVDLRVLAFTLGVALLSWLVFGLAPAIQGSQADLRRDLTLRRGDAGGARRGRNLLVVA
                     EIALSLVLLAGAGLLMKSFLRLLSVDPGFETGHLLVARLSLPGDRYAGREKTAAFCEA
                     LRLRLTGLPGVEEVGVVSALPLAANRASVPFTIEGRPAQPGEEPWTNYRLADDGYFRA
                     FGIPIVAGRPFGDRDRPDGPPVAIVSRTFASRFLAGADPIGTHLRIEDNDRGPRPVEI
                     VGVAGDVRHAGLESDPEPHLYLPLQQAHEDSVGLLTGNQYWLVRARMEPLALSSAVRG
                     ALRDVDPDVPATSLRSMEDYLQASVSFRKFSLRLVAAFAVTALLLAATGLYGVVAYGV
                     SRRTHEIGIRMTLGALRRDILRMVVGEGMRLAMLGTALGLAAGLALTRSMKSLLFGVG
                     AADPWTFAGITVLLLAAALLACWLPARRATRVTPVIALRSE"
     gene            3133..3558
                     /locus_tag="VFD06_11795"
     CDS             3133..3558
                     /locus_tag="VFD06_11795"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZN04255.1"
                     /translation="MTTAASRETRHEGSCLCGRVRYRIEGPVGNMSNCHCTDCRKHSG
                     AAFVTFVEAPKKALAFVEGEDQLTTHQASSGTKRSFCRNCGSSVVCFVDADDFVEIDA
                     ATLDTPLSLRPEYHIYVRSKVSWFDIQDGKPKHQASSRG"
     gene            complement(3575..4693)
                     /locus_tag="VFD06_11800"
     CDS             complement(3575..4693)
                     /locus_tag="VFD06_11800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN04256.1"
                     /translation="MRREPASRRPERRILFVIPSLEGGGAERVVTHLLRGLASRTFDL
                     HLALFAASGAFMADVPDSVTIHDLGSRRVRNGLPSLLRLVWRLRPDVVVPATFTVPLM
                     MGLRPFFPRSVALLARESLLPSNGLRAWRPWLPWPLPRLLYRQATGVICPAAAMAEEL
                     VRNFGVPAERIHHIPNPVDAGAIARRADGSPSPYAGPGPHLLSIGRLEPQKGFDRLLS
                     VLARLGPAGHLWILGTGNSGGPLREEANRLGLASRVHFQGFVANPFVWLRHADLFVQG
                     SRFEGSPNALLEALACGTRVVAFDSPGGTREILEGLPGTALVADGDTDAFATAVRGML
                     SASRPAPPALPARHAYEVVMRQYERLLSGDRDAACSGL"
     gene            complement(4690..>5220)
                     /locus_tag="VFD06_11805"
     CDS             complement(4690..>5220)
                     /locus_tag="VFD06_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04257.1"
                     /translation="GRAARDEDRRAYLRGVWGVQCAALLTYSMAGFVKLMTGAWHLAT
                     GQGGVLTPAAMALHVANSGIRFGIDAPLADLAIRARILGLPIYLVVLYLELFALPIAF
                     RPRLHRPWGVALVLMHLGISLTLNLFFLSSLFIVGLLLLASPFAPATPGWRAAAGELP
                     LLGRFVRRFAPAPASL"
BASE COUNT          751 a         1878 c         1888 g          703 t
ORIGIN      
        1 gagcgccgag ctgctgatcc tgtcgctcgt cgaggcccgc ccccggcacg gctacgagat
       61 cagccggcag atcgagacgc ggtcgggggg ggcgctgcgc ttccacgtgg cgtcgctcta
      121 tccgctgctc taccggctgg agcgacgcgg ctggatcgcc ggccgctggg tggagaaagc
      181 ggggcagcgg cggcgccgct actaccgcct gacccgcgaa ggacggaaga tcctgacggc
      241 gcagcgcagc acctgggagt cgttcgtcgc ggcgatcgcg cgcatcaccc gtccggagcc
      301 gtcggaggga tagaggatgc ccgactggag ggacgagatc cggcgacgcc tgccgcctga
      361 gacgcgcggc gtcgggcgcc gcgagggggt cgtcgaggag ctggcgcagc acctggagga
      421 cgtccacgcg gcgctcctcg cggagggggt ctctcccgaa gaagccgaac gacggacgcg
      481 cgccgagctt catggaagcg gcgcggagga ccgcctgacc gcgccgggcg gcgcaccgcc
      541 ggcccctccg ctggaggcgc tcgcgaaggg aagcctgctc ggcgatctcc ggcaggacct
      601 ggcccacggc ctccgactgc tgcgcgccca gttcggcttc gcggccatcg cgatcctcac
      661 tctgggcctc ggcaccggcg ccgtcgctgc gatcttcagc atcgtcaacg cggtgctcct
      721 gcgcccgctg ccgttccgcg acccggaacg gctggtcgcg gtctcgctcc ggcgcgccga
      781 cgacgtcagg cacccgttca acctgccgga ctttctcgac gatcgcgacc agaaccgcac
      841 gctcgaaggg atcgcggcct atgggaactg gagcggcaac atgacgggcg tcggcgagcc
      901 ggagcggctc tcgggcctgc gcgtctcggc ggatctgttc gagctgctcg gtgtgcgcgc
      961 gctcgccggt cgcacgcttc tcgccggcga cgaccggccg gagaaccggc gcgtcgtggt
     1021 cctgggccac tcgctctggc ggcgccgctt cggaggggat ccgggggtgg tggggaggac
     1081 gatcgtcctc aacggcgacg gatacgaagt ggtcggggtc ctgcccgcct tcttcccatt
     1141 cccgattccg gaggcggagc tggcgatccc gctcgcgccg gacgccgacc cgcagcgaag
     1201 cgtccggtcc tcggtgaact tcctgcgcgc cgtcgcgcgt ctcgggcccg gcgtcacccg
     1261 cgagcaggcc gagtccgacc tcaacaccct cgcgcagcag cagcgccggg agcacccggt
     1321 cgagaacgcg cagaagatgg gaaggacgct cacgccgctc cacgaggagc tcgtcggcgg
     1381 gttcggtccg gcgctgtgga ttctcttcgg agcggtcggg ctggtcctcc tcatcgccag
     1441 cgtcaacctg gccgggctgg cactggcgcg cgccgccgcc cgccggaagg agatggcgat
     1501 ccgcgccgcc ctcggtgcca cccgccgtcg actggcgcgg cagatcgcgg ccgaatgtct
     1561 gctgctggcg ctcttcggcg ccgccgccgg gatcgtcgtc gccgtcttcg gcgtcgatct
     1621 gctgctggcg atgagccccg ccggcctgcc gcgcgcggcg gaggtgcacg tcgatctccg
     1681 tgtcctcgcc ttcacgctcg gggtcgcgct cctctcctgg ctcgtcttcg gtctggcccc
     1741 cgccatccag gggtcgcagg ccgacctccg ccgcgatctg acgctgcggc gcggcgatgc
     1801 gggcggggcc cgccgcggtc ggaacctgct ggtcgtcgcg gagatcgcac tctcgctcgt
     1861 gctgctggcc ggcgccggac tcctgatgaa gagctttctg cggctcctct ccgtcgatcc
     1921 gggtttcgaa acggggcacc tgctcgtcgc gcgcctgtcg ctccccggag atcgctacgc
     1981 cgggagggag aagaccgcgg cgttctgcga ggcgctcagg ctgcggctca ccggtctccc
     2041 cggcgtcgaa gaggtgggtg tggtctccgc cctgccgctc gccgccaacc gggcgagcgt
     2101 cccgttcacc atcgaggggc gcccggcgca accgggggag gagccctgga cgaattaccg
     2161 gctcgccgat gacggctact tccgcgcctt cgggatcccc atcgtcgccg ggcgcccgtt
     2221 cggcgatcgg gaccggcccg acgggccgcc ggtcgccatc gtcagccgga ccttcgccag
     2281 ccggttcctc gcgggcgcgg atccgatcgg gacgcacctg cgcatcgagg acaacgaccg
     2341 cggcccgcgc ccggtggaga tcgtcggggt ggcgggggac gtccggcacg ccggcctcga
     2401 gagcgatccg gagccgcacc tctacctgcc gctgcagcag gcgcacgagg actcggtcgg
     2461 gctcctgacc ggcaaccagt actggctggt gcgcgcgagg atggaaccgc tcgcgctgtc
     2521 ctccgcggtg cgcggcgcgc tccgcgacgt cgatccggac gtcccggcca cgagccttcg
     2581 ctccatggag gactatctcc aggcgtcggt ctccttccgg aagttcagcc tccggctcgt
     2641 ggcggccttc gcggtcacgg ccctcctcct ggccgccacc ggcctgtacg gcgtcgtcgc
     2701 ctacggcgtc agccgccgga cgcacgagat cggcatccgc atgacgctcg gcgcgctgcg
     2761 ccgggacatc ctccggatgg tggtcggcga ggggatgcgg ctggcgatgc tggggaccgc
     2821 gctggggctc gcggccggcc tggcgctgac ccgatcgatg aagagcctgc tcttcggcgt
     2881 gggcgcggcc gatccctgga ccttcgccgg gatcaccgtc ctgctgctcg cggccgcgct
     2941 tctcgcgtgc tggctcccgg cccgccgcgc cacgcgcgtg acgccggtca tcgcgctgcg
     3001 atcggagtag ccgccgacgc gacgcgcctg accgacgccg acgcgacgcg ctcagccgac
     3061 cccgacacgt ccgtccgacc ttgacagccg cggagcggtg gggctagggt cgccgcaccg
     3121 ggaggaactc cattgacgac cgcggcatcg agagaaaccc gtcacgaagg ctcctgcctg
     3181 tgcggccgcg tgcgctaccg catcgagggg ccggtgggca acatgtcgaa ctgtcactgc
     3241 accgactgcc gcaagcacag cggggccgcc ttcgtcacct tcgtcgaggc gccgaagaag
     3301 gcgctcgcgt tcgtcgaggg ggaggatcag ctgacgacac accaggcgtc ctccggcacc
     3361 aagcgcagct tctgccggaa ctgcggctcg agcgtcgtct gcttcgtgga cgcggacgat
     3421 ttcgtcgaaa tcgatgccgc gacgctcgac acccctttga gcctgcgccc cgagtaccac
     3481 atctacgtgc gcagcaaggt ctcctggttc gacatccagg acggcaagcc gaagcaccag
     3541 gcctcgagcc ggggctgaaa caagggaagg gacctcacag accgctgcaa gcggcgtccc
     3601 gatcgccgga gagcagacgc tcgtactgtc gcatcacgac ctcgtaggcg tggcgcgcgg
     3661 gaagcgcggg cggtgcggga cgggacgccg acagcatgcc gcgcacggcc gtcgcgaagg
     3721 cgtccgtgtc tccatccgcc acgagggccg tgcccggaag cccctcgagg atctcgcggg
     3781 tgccgccggg cgagtcgaag gcgacgaccc gagttccgca cgccagcgct tcgagcagag
     3841 cgttcggcga gccctcgaag cgcgagccct gcacgaacag gtccgcgtgg cgcagccaga
     3901 cgaagggatt ggcgacgaag ccctggaagt gaacccgcga tgcgagaccg aggcgattcg
     3961 cctcctcgcg cagcggcccg ccgctgttcc cggtccccag gatccagaga tggcccgccg
     4021 ggccgaggcg agccagcacc gacaggaggc gatcgaatcc cttctgcggc tccagccggc
     4081 cgatgctgag caggtgcggc cccggtcccg cgtagggcga gggggagccg tccgcccgac
     4141 gggcgatcgc gccggcgtca accggattcg ggatgtggtg gatccgctcc gccgggacgc
     4201 cgaagttccg gaccagttcc tccgccatgg cggccgccgg acagatcacc ccggtcgcct
     4261 gcctgtagag caggcggggc agcggccacg ggagccacgg tcgccacgcc cgcagtccgt
     4321 tgctcggcag caggctctcg cgcgccagga gcgcgacgct gcgcgggaag aagggtcgta
     4381 gtcccatcat gagcggtacg gtgaaggtcg cgggcacgac gacgtccggg cgaaggcgcc
     4441 agaccagtcg cagcagcgac ggcaggccgt tccggacgcg acggctcccc aggtcgtgga
     4501 tcgtcacgga gtccggcacg tccgccatga aagctccgga ggcggcgaac agcgccagat
     4561 gcaggtcgaa ggtccgcgat gcgaggccgc gcagaaggtg cgtgacgacc cgttccgcgc
     4621 cgccgccttc gagggaggga atgacgaaca ggatgcgtcg ttccggccga cgggacgccg
     4681 gttcgcgcct cacagggacg caggcgcagg agcgaaccgg cggacgaaac ggccgagaag
     4741 cggcagttcg cccgcagcgg ctcgccagcc cggcgtcgcg ggcgcgaacg gcgacgcaag
     4801 cagcagcagg ccgacgatga acagcgagga caggaagaac aggttcaggg tcaggctgat
     4861 gccgaggtgc atcagaacga gcgccactcc ccagggccgg tgcagccgcg gccggaacgc
     4921 gatcggcagc gcgaacagct cgaggtagag caccacgagg tagatcggca ggcccaggat
     4981 gcgcgcgcgg atcgccaggt cggcgagcgg cgcatcgatg ccgaagcgga tgccggagtt
     5041 cgccacgtgc agcgccatcg ccgccggcgt cagcacgccc ccctgtccgg tggcgaggtg
     5101 ccacgctccg gtcatgagct tcacgaatcc cgccatcgag tacgtcagga gcgccgcgca
     5161 ctgcacgccc cagacgccgc gcaggtacgc ccgccggtcc tcgtcccgcg ccgcccggcc
//
LOCUS       DASRIQ010000485         5634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1754391, whole genome shotgun sequence.
ACCESSION   DASRIQ010000485 DASRIQ010000000
VERSION     DASRIQ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754391"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VFD06_11810"
     CDS             complement(<1..1172)
                     /locus_tag="VFD06_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN04258.1"
                     /translation="MTQPFDLIGLLVILLTVACAVLLITTRLRLPAVVGYLVSGVVVG
                     PHVLGHLTESDTISSLAEIGVLLLMFTIGLEFDTQYFLRIRRVAIGGGALQIGLTILV
                     AMGAGLLLGWTPRAAATIGCILALSSTAIVLKSLMDLGLLDSLAGRLSLGILIFQDLS
                     IVPMMILLPVGGGSTGTIVLNLAAALGRAGFLLAVTLILSRRVMPRFLHMMASSRSQE
                     TVLLATLTLCLGLAWVSHLLGVSYALGAFLAGLILGGTEYAHQMRANVVPFRDIFAGV
                     FFVAMGMLLDPEFALSAWREVLLVTLFVVVGKTLLTAASITLFGFPWTVALQTGLNLA
                     QVGEFSFLLMIMGRRTGILSEEIYKVAIACSVISMLVAPILTRWSPRLSTRLANATG"
     gene            1232..1320
                     /locus_tag="VFD06_11815"
     tRNA            1232..1320
                     /locus_tag="VFD06_11815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1266..1268,aa:Ser,seq:tga)
     gene            complement(1376..1891)
                     /locus_tag="VFD06_11820"
     CDS             complement(1376..1891)
                     /locus_tag="VFD06_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04259.1"
                     /translation="MTAPTNIRPAAANVRAIVARFADGRTIKGTTHDFAPDKDIFHVY
                     VDGDESKPAVTIRISALKAIFFVKTYQGDSSYVEDKSFRPGADQGRRIRVIFKDKEEI
                     AGFAPGSVAGRSGFFVLPVDRASNNSRIFIVHAAVAKKEFPSKDAEPKVDADATDPSP
                     VAAAGGAKGRG"
     gene            2025..3353
                     /locus_tag="VFD06_11825"
     CDS             2025..3353
                     /locus_tag="VFD06_11825"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZN04260.1"
                     /translation="MRGIGRKMGIGVLAGAALLGAAVLLDAAAPIRAASDDIAARARR
                     LHAQAIVVDTHVDAPDQLLEKWADLGERGATDHFDIPRARQGGVTAPFFAVYIPATYA
                     ETGGAARQTLEVIDIVGRAVASHPDDFVPAASVAQIRRAKKDGRIAILMGIEGGHAIE
                     DSLAALRQFHRLGVRYMTLTHTNTNHWADSTGPFYLPDYDPAAARQHQGLTDFGREVV
                     TEMNRLGMMVDISHVSDDTIADVLETSRAPVFASHSSCRALCDIPRNLTDDQIKRVAA
                     AGGVVMINIGSYFLDPESVAAAKAEVAKVRPEYERIKKEMAGDPKGRDAAIGKLLKPL
                     PRRRTQWTKAIDHIEHAMKVGGPGCVGLGTDFDGVRDPPEGLDDVSMLPKITEELLRR
                     GHSEAEVRGVLGENFLRFFARVEETAARLSGEAPSTATIAGADQGARRSD"
     gene            complement(3329..4816)
                     /locus_tag="VFD06_11830"
     CDS             complement(3329..4816)
                     /locus_tag="VFD06_11830"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZN04261.1"
                     /translation="MDQPPAPRPRDSYALAGSLFLRALAVVYLMAFVSLAVQIRGLAG
                     ANGILPAAQYLEALGAQVGPERYWHVPTLFWLTGASDGVLVAACWAGAAVALMVACGF
                     LPLPGLIACFALYLSLSSGTRTFLNFQWDILLTEAGLLALFVAPWRLRAPVPSLPAPA
                     APALWLVRWLLFRLMFSSGVVKLTSGDPTWWNLRALDVHYFTQPIPTWTAWFVHQLPA
                     WFHTFECGVMFCIELVVPFGLFAPRPVRRVCALILFGFQAMIAATGNYAFFNYLTMAL
                     CLVLFDDAAWPAWLRRRLPATDSAVPARRWPLWVSIPVLTVFVTLTTAAMTSRWDMPL
                     GWPSPVEKALTLVRPLRIINAYGLFAVMTTTRQEIVVEGSDDGQNWSAYEFKWKPGDV
                     SRRPGFVAPHQPRLDWQMWFAALSSYRQQPWFANFLGRLLQGSPEVLALLEKNPFPDR
                     PPRYIRSVVYDYRFSDFGVRRESGAWWVRTPEGQYSPVQSLRRAP"
     gene            4906..>5634
                     /locus_tag="VFD06_11835"
     CDS             4906..>5634
                     /locus_tag="VFD06_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN04262.1"
                     /translation="MPLTLATVAALLLSGLIGISLGLLGGGGSILALPVLVFVARLDP
                     RQAVGMSLAIVGATSLTTSLLHRRSGRVDTRTGALFGGVGLPAAFLGARLTHLLPPRT
                     LLLCFGILMLVVGTLMVARALREKHEAVAVRPRRPAAVLAAGAGVGFLTGFLGIGGGF
                     LIVPALTLFAGLAMPVAVGTSLLVIAFNCAAGFLGHLEAAAIPIVPTLSFMGMAIAGG
                     VVGERMAGRASPRRLRRAFGIFIIL"
BASE COUNT          925 a         1889 c         2061 g          759 t
ORIGIN      
        1 cctgtcgcgt tggcgaggcg cgtggagagc cgcggactcc agcgggtcag gatcggcgcc
       61 accagcatcg aaatgaccga gcaggcgatg gcgaccttgt agatctcctc cgacaggatc
      121 ccggtgcgcc gccccatgat catcagcagg aaggagaact cccccacctg ggccaggttg
      181 agcccggtct gcagcgccac cgtccacgga aagccgaaca gcgtgatcga cgcggcggtg
      241 aggagcgtct tgccgacgac gacgaagagg gtgacgagca gcacctcgcg ccaggcggag
      301 agggcgaact ccgggtcgag cagcattccc atcgcgacga agaagacgcc ggcgaagatg
      361 tcgcggaacg gcacgacgtt ggcgcgcatc tggtgcgcgt actcggtccc tccgaggatg
      421 aggccggcga ggaacgcccc gagggcgtac gagacgccga gcaggtggct cacccaggcg
      481 aggccgaggc agagagtgag ggtcgcgagc aggaccgtct cctggctgcg cgacgacgcc
      541 atcatgtgca ggaagcgcgg catcacccgc cggctgagga tgagagtgac cgccagcagg
      601 aacccggcgc gtccgagcgc ggcggccagg ttcagcacga tcgtgccggt ggagccgccg
      661 ccgaccggca agaggatcat catcgggacg atgctcaggt cctggaagat caggatgccg
      721 aggctgaggc ggccggcgag cgagtcgagc aggccgaggt ccatcaggct cttgaggacg
      781 atggcggtgc tgctcagcgc gaggatgcag ccgatcgtcg ccgcggcgcg cggtgtccag
      841 ccgagcagga gccctgcccc catcgcgacg agaatggtca ggccgatctg cagcgcgccg
      901 ccgccgatgg cgacgcggcg gatgcgcagg aagtactgcg tatcgaactc cagcccgatg
      961 gtgaacatca ggaggaggac gccgatctcg gcgagggacg agatggtgtc gctctcggtc
     1021 aggtggccca ggacgtgcgg gccgacgacg accccggaaa cgaggtagcc gacgaccgcc
     1081 ggcagccgca ggcgcgtcgt gatcagcagg acggcacaag cgacggtcaa aaggatgacg
     1141 agcaggccga tcaggtcgaa gggctgcgtc atgagcggtg tgcgtctggc cggccgccgg
     1201 cggggccggc gccgcaccta taatcacccc tggagggatg gccgagcggt ttaaggcggc
     1261 ggtcttgaaa accgctggcc cctcgtgggc ccgtgggttc gaatcccact ccctccgcca
     1321 cccgcctccc atccggcgtc aaccgatccg gagggatcct acacgtcgga tgtggctagc
     1381 cgcgaccctt cgcgccgccg gcagccgcga ccggagaagg gtccgtggcg tcggcgtcca
     1441 ccttcggctc cgcatccttc gatgggaact ccttcttcgc caccgcggcg tgcacgatga
     1501 agatgcgcga gttgttgctc gcccgatcga ccggcaggac gaagaagccg gagcggccgg
     1561 cgaccgagcc gggggcgaag ccggcgatct cctccttgtc cttgaagatg acccggatgc
     1621 gccggccctg gtcggcgccc ggacggaagc tcttgtcctc gacgtaagag ctgtccccct
     1681 ggtaggtctt cacgaagaag attgccttca gggccgagat ccggatggtc accgccggct
     1741 tcgactcgtc gccatccacg tagacatgaa agatgtcctt gtcgggggcg aagtcgtgcg
     1801 tcgtcccctt gatggtgcgg ccgtcggcga aacgggcgac gatggcgcga acgttggcgg
     1861 ctgcgggccg gatgttcgtc ggtgcagtca tcggggctgg cctcccccgg ggcatgggca
     1921 gacccgggcc cggtccaaga tggtgcccgg gcccgccgcc gtcaaacggg tgttacgctt
     1981 tcgcgctcgg cgggccggcg cccgcccgtg aggagggtcg gagcatgcgg gggatcggcc
     2041 ggaagatggg aatcggcgtg ctggcggggg cggcgctcct gggcgcggcg gtcctcctgg
     2101 atgcggcggc gccgatccgg gccgcctcgg acgacatcgc ggcgcgggcg cgccggctgc
     2161 acgcgcaggc gatcgtcgtg gacacccatg tcgacgcacc ggaccagctc ctggagaagt
     2221 gggccgatct cggcgaacgc ggagcgaccg accacttcga cattccgcgc gcccgccaag
     2281 ggggggtgac ggcgccgttt ttcgccgtct acatccctgc cacgtacgcc gagacggggg
     2341 gcgcggcgcg tcagacgctc gaggtcatcg acatcgtggg gcgcgcggtg gcatcgcacc
     2401 cggacgactt cgtgccggcg gcgtcggtgg cgcagatccg gcgcgccaag aaagatgggc
     2461 gcatcgccat cctgatgggg atcgaagggg ggcacgccat cgaggactcg ctcgcggcgc
     2521 tgcggcagtt ccaccggctc ggggtgcgtt acatgacgct gacgcacacc aacaccaacc
     2581 actgggctga ttccaccggg ccgttctacc tgcccgacta cgacccggcc gccgcccggc
     2641 agcaccaggg gctgaccgac ttcggccgcg aggtggtgac ggagatgaac cgcctcggga
     2701 tgatggtgga catctcgcac gtctccgacg acaccatcgc cgacgtgctg gagaccagtc
     2761 gcgcgccggt gttcgcttcg cactcctcct gccgggcgtt gtgcgacatc ccgcggaacc
     2821 tgaccgacga tcagatcaag agggtcgccg ccgcgggtgg cgtcgtgatg atcaacatcg
     2881 ggtcgtattt cctcgacccg gagtcggtcg ccgccgcgaa ggcggaggtc gcgaaggtcc
     2941 gacccgaata cgagcggatc aagaaggaga tggcgggcga cccgaagggg cgcgacgccg
     3001 ccatcggcaa gctgctcaag ccgctgccgc gccggcgcac ccagtggacg aaggcgatcg
     3061 accacatcga gcacgccatg aaggtgggtg ggccggggtg cgtcggcctc ggcaccgact
     3121 tcgacggcgt ccgcgacccg cccgagggac tcgacgacgt gtcgatgctg ccgaagatca
     3181 ccgaggagct gctgcgccgc ggccattccg aggcggaggt gcgcggcgtc ctcggcgaga
     3241 acttcctgcg ctttttcgcc agggtcgagg agaccgccgc ccgcctgagc ggcgaggcac
     3301 cttccaccgc cacgatcgcc ggcgccgatc agggagcccg gcggagcgac tgaaccgggc
     3361 tgtactgtcc ctcgggggtg cggacccacc aggcgccgct ctcgcgccgg acgccgaagt
     3421 cggagaagcg gtagtcgtag acgaccgagc ggatgtagcg cggcgggcga tccgggaacg
     3481 ggttcttttc gagaagcgcg agcacctcgg gggaaccctg cagcagccgc ccgaggaaat
     3541 tcgcgaacca cggctgctgg cggtaggagg acagggccgc gaaccacatc tgccagtcga
     3601 gccgcggctg gtgcggtgcc acgaacccgg gcctccggga gacgtctccc ggcttccact
     3661 tgaattcgta ggccgaccag ttctggccgt cgtcgctccc ctccacgacg atctcctgcc
     3721 gcgtcgtggt catcaccgcg aacaggccgt aggcgttgat gatgcgcagc ggccggacga
     3781 gggtcagggc cttttcgacc ggcgacggcc acccgagcgg catatcccag cgagacgtca
     3841 tcgccgccgt cgtcagcgtg acgaacaccg tcagcaccgg gatcgagacc cacagcggcc
     3901 agcggcgcgc cggcacggca gagtcggtcg ccggaagccg gcggcgcagc catgcgggcc
     3961 aggcggcgtc gtcgaagaga acgaggcaca gcgccatcgt caggtaattg aagaaggcgt
     4021 agttgccggt cgcagcgatc atcgcctgga agccgaaaag aatcagcgcg cagacgcgcc
     4081 gcaccgggcg cggcgcgaag aggccgaacg gcacgaccag ctcgatgcag aacatcacgc
     4141 cgcactcgaa cgtgtggaac cacgccggca actggtgcac gaaccaggcg gtccatgtcg
     4201 ggatcggctg cgtgaagtag tgcacgtcga gggcgcgcag gttccaccag gtcgggtcgc
     4261 cgctcgtgag cttcaccact cctgacgaga acatcagccg gaagaggagc cagcgcacca
     4321 accacagcgc cggcgccgcc ggagcgggga gcgacggcac cggggcccgc agccgccacg
     4381 gcgccacgaa cagcgccagc agtccggctt ccgtcaggag gatgtcccac tggaaattga
     4441 ggaaggtgcg ggtcccggac gacagcgaca ggtagagggc gaagcacgcg atcagtcccg
     4501 gcagcggcag gaagccgcac gccaccatca gcgcgacggc cgccccggcc cagcaggcgg
     4561 cgacgagcac gccgtccgaa gcgccggtca gccagaaaag cgtcggcacg tgccagtagc
     4621 gctccggccc gacctgcgca ccgagcgcct cgagatactg cgccgccggc aggatcccgt
     4681 tcgcaccggc caggccgcgg atctgcaccg cgagcgagac gaacgccatc aggtagacga
     4741 ccgccagggc gcgcaggaag agggagccgg cgagggcgta ggagtcgcgc gggcgcgggg
     4801 ccgggggctg gtccatcggc cgggaacgat acaacgtgcc gacccgagcg gagcgatccc
     4861 gcgccacgtt gcacaggggg gcgagaccgt gcaggatcgg tcgccgtgcc gctgaccctc
     4921 gccaccgtcg ccgccctcct cctcagcggt ctcatcggga tctcgctcgg tctcctgggc
     4981 gggggtggat cgatcctggc gctgccggtg ctggtgttcg tggcgcggct cgatccgcgc
     5041 caggcggtgg ggatgtcgct cgccatcgtc ggcgccacca gcctcacgac gagcctgctg
     5101 caccgccgga gcgggcgcgt cgatacgcgc accggagccc tgttcggcgg cgtcggcctg
     5161 ccggcggcgt tcctcggcgc gcgcctgacg caccttctcc cgccccgcac gctgctcctg
     5221 tgtttcggga tcctgatgct ggtcgtcggg acgctgatgg tggcgcgggc gctgcgggag
     5281 aagcacgaag cggtcgcggt ccggccgcgg cgtcccgcgg cggtgctcgc cgccggcgcc
     5341 ggcgtcggct tcctgaccgg cttcctcggg atcggcggcg ggttcctcat cgttccggcg
     5401 ctgacgctgt tcgcggggct cgcgatgccg gtggcggtcg ggacgtcgct cctcgtcatc
     5461 gccttcaact gcgccgcggg gttcctcggg cacctggagg cggcggcgat tccgatcgtc
     5521 ccgacgcttt cgttcatggg gatggcgatc gccgggggcg tcgtcgggga gcggatggcc
     5581 gggcgcgcgt caccgcggcg gctgcggcgc gccttcggga tcttcatcat cctc
//
LOCUS       DASRIQ010000486         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_726321, whole genome shotgun sequence.
ACCESSION   DASRIQ010000486 DASRIQ010000000
VERSION     DASRIQ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726321"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..2620)
                     /locus_tag="VFD06_11840"
     CDS             complement(107..2620)
                     /locus_tag="VFD06_11840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04263.1"
                     /translation="MDDLIQDLKYGARMLRKRPGFTLVAVLTLALGIGANSAIFSSVN
                     AILLQPLPLRDRDRLVLFDATTSEGTSVGSPSDEVWHRWSNASYLHLKENVPALEDVA
                     AFRSGETRLNLLGEEGGAAGAQLATGHLVSGNYFSLLGVPAVLGRPLAPDDDREAAPP
                     VAVISHACWKTRYAGDPAVVGRAVTVNGTPLTIAGVMPEWFFGLRIRRAADFWLPLAL
                     QPRIERTTESYLAERDVYWLNLVGRLKPGSDIAAANAQVNVALKQYLRSAAGTTPEED
                     WQRAIDHGTIRLAPAGPGISGLRVHYGEPLKVLMAVAGFVLLIACANLTNLLLSRATE
                     RRAEIAMRLAMGAARGRLIRQALTESLMLAALGGAAGLLLALWGVEALRTLVSKSAPI
                     DVGLNLPVLAFTAGVSILAGLLFGLAPALRTGRADLSDALRSRGETGGGRLRSGLGPA
                     LVATQVALSLVLLVGSGLLVRSLVNLAGAEVGFQRDGVAVLDIDTRVAGLQTSELSDY
                     YRRLLERVGGVPGVKSATVATYSPMNGTSRSSSLTVEGYTSTPGDAPEANVILVGPRY
                     AETLGIPIVKGRIIDDRDGPAATKVAFVNEAFARAYFAGADPIGRRVSIGDEPENPRY
                     EVIGIVGDASYDSPREAPARMLYLGILQEQDQSAYASDLEVRTAGDPMTAVTDLRHAV
                     GEVDGRVPIASVTSLRAQVADSVSAETLLAQLVSVFSVLALVLACVGLYGVVSQAVAR
                     RTNEVGIRMALGADRRRILTMVLGEAGRLILAGLAVGIPGAAIAARLLTSQLYGVAPA
                     DPLTIASSAGLLLAVALFAGYIPARRASRVEPLVALRAE"
     gene            2866..>3472
                     /locus_tag="VFD06_11845"
     CDS             2866..>3472
                     /locus_tag="VFD06_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04264.1"
                     /translation="MRRNRSLLIASAVAILLARPGISDTPLPTPLVEQTRSDLVLIEV
                     YARDWKGNVVRDLRVDELVLKVDQNRAPRKIESLEFVEPTSEGVIPDAAIEEGGDAPL
                     VQDASTLSAGQARRQRPRRFMLFFDDDTSYPVNMAEARRAALRFLQGSGLATDQFSVV
                     VYEPARKVEVLCPFTDDRGEITRALEASLEDPRRFSDYATER"
BASE COUNT          525 a         1221 c         1250 g          476 t
ORIGIN      
        1 gcccggagcc cgagtgcgag cgcgtggaac atgtcgtcca tccgctccgg cgtgtagggc
       61 tcgccggagt cgatccagtg ccgcagcagg gcgaagagcg cgagcgctac tcagccctga
      121 gcgccacgag cggctcgacg cgcgaggcgc gccgcgccgg gatgtagccg gcgaacaggg
      181 cgaccgcgag cagcaggccg gcgctcgagg cgatcgtcag cgggtcggcc ggggcgacgc
      241 cgtagagctg gctcgtgagc aggcgcgccg cgatcgccgc tcccggaatt ccaacggcga
      301 ggccggcgag gatgagccgt ccggcctccc ccaggaccat cgtcaggatg cgacggcggt
      361 cggcaccgag cgccatgcgg atgccgactt cgttcgtgcg ccgcgccacc gcctgcgaca
      421 ccaccccgta caggccgacg caggccagaa cgagcgccag gacgctgaac acgctgacga
      481 gctgggcgag aagggtctcg gcgctcacgg aatccgcgac ctgggcccgg agcgacgtca
      541 ccgaggcgat cgggacgcgg ccgtccacct cgccgacggc gtggcgcagg tcggtgaccg
      601 ccgtcatcgg gtcgcccgcc gtgcggactt cgaggtcgct ggcgtaggcg ctctggtcct
      661 gctcctgcag gattccgaga tacagcatcc gcgccggggc ttcccgcggg ctgtcgtagc
      721 tggcgtcgcc gacgatgccg atcacttcgt accgcgggtt ctccggctcg tcgccgatgg
      781 acacgcgccg gccgatcggg tcggccccgg cgaagtaggc tcgggcgaac gcctcgttga
      841 cgaaggcgac cttggtcgcg gccgggccgt cccgatcgtc gatgatgcgt cccttcacga
      901 tcgggattcc cagcgtctcg gcgtagcgcg gcccgaccag gatcacgttc gcctcgggcg
      961 cgtcgcccgg cgtggaggtg tatccctcga cggtcaggct gctgctgcgg ctcgtgccgt
     1021 tcatcggact gtaggtggcg accgtggccg acttcacccc gggaacccct cccacacgct
     1081 cgagcaggcg gcggtagtag tcgctcagct cggatgtctg aagaccggcg acccgcgtgt
     1141 cgatgtcgag caccgcgaca ccgtcgcgct ggaagccgac ctccgccccg gccaggttga
     1201 cgagggagcg caccaggagg cccgagccga cgaggagcac cagcgacagc gccacctgcg
     1261 tcgccacaag tgccggcccc agccccgacc gcagccggcc gccgccggtc tcgccgcggg
     1321 agcgcagggc gtcggagagg tcggcccgac cggtccgcag ggccggcgcc aggccgaaga
     1381 ggagtccggc gagaatcgac acccccgccg tgaaggcgag gaccggcagg ttcagtccga
     1441 cgtcgatcgg ggcgctcttc gacacgaggg tgcgcagcgc ctccacgccc cagagggcca
     1501 ggagcaggcc ggccgcgccg ccgagcgccg ccagcatcag gctctcggtc agcgcctgac
     1561 ggatgagtcg gccgcgcgcc gcccccatgg cgaggcgcat ggcgatctcg gcgcgcctct
     1621 ctgtggcccg cgacagcagg aggttggtca ggttggcgca ggcgatgagg agcacgaacc
     1681 cggcgaccgc catgagcacc ttgagaggct ctccgtagtg gacgcgcagg ccggagatcc
     1741 cgggacccgc cggcgccagc cggatcgttc cgtgatcgat cgcccgctgc cagtcctcct
     1801 ccggcgtggt gcccgccgcg ctgcgcaggt actgcttcag cgccacgttc acctgggcgt
     1861 tcgccgccgc gatgtcgctc cccggcttca gccgtccgac gagattcagc cagtagacgt
     1921 cgcgctcggc caggtaggac tcggtcgtcc gctcgatgcg gggctgcagc gcgagtggaa
     1981 gccagaaatc ggcggcgcgg cggatgcgca gtccgaagaa ccactccggc atgacgccgg
     2041 cgatcgtcag cggcgtgccg ttgaccgtga cggcgcggcc gacaacggcg ggatccccgg
     2101 cataacgcgt cttccagcag gcgtgcgaaa tcacggcgac cggcggggcc gcctcccggt
     2161 cgtcgtcggg agcaaggggc cgcccgagca ccgccggcac cccgagcaga gaaaagtagt
     2221 tgccggagac gagatgcccc gtcgccagtt gtgctccggc cgcgcccccc tcctcgccga
     2281 ggaggttcag gcgagtctcg ccgctccgga atgccgccac gtcctcgagt gccggaacgt
     2341 tctccttgag gtgcaggtac gaggcgttcg accagcgatg ccagacttcg tcgctcgggc
     2401 tcccgacgct cgtcccctcc gaggtggtgg cgtcgaacag gaccaggcga tcgcggtccc
     2461 gcagcggcag cggctgcagc aggatggcgt tgaccgagga gaagatcgcc gagttggcgc
     2521 cgatgccgag cgccagggtc aggacggcga cgagcgtgaa gccgggacgc ttgcggagca
     2581 tccgggcgcc gtacttcagg tcttgaatca ggtcgtccaa gagactctcc tcttcaagcg
     2641 agccgacgct cgcgaggcaa gttgcgtgcc ttgctctccg gcgggcagga cgcaggttcg
     2701 tcggggactt ccgcgcgcgt cccgggcccc cgctgtccgg tcccgggacg accccgtccc
     2761 acgagcggcg cggagaatgg accgcggggt ccgctgcggc ggagggtgaa tttggctttc
     2821 ggcggggggg cgatgtcata atccggccgc cggaggtctt ccttcatgcg ccgaaaccgc
     2881 agcctcctca tcgcatccgc cgtcgccatc ctcctcgcca ggccggggat ctccgacacg
     2941 ccgctgccga cgccgctcgt cgaacagacg cgcagcgatc tggtcctcat cgaggtgtac
     3001 gcgcgtgact ggaaggggaa cgtcgtgcgc gatctgcggg tggacgagct ggtgctgaag
     3061 gtggaccaga accgggcccc ccggaagatc gaatcgctcg aattcgtcga gccgacgtcc
     3121 gagggggtca ttcctgatgc ggcgatcgag gaagggggcg acgcgcctct cgtccaggac
     3181 gcgtccacgc tcagcgccgg ccaggcgcgc cggcagcgac cgcgccgctt catgctgttc
     3241 ttcgacgacg acacctcgta cccggtcaac atggccgaag cgcggcgggc ggcgctccgc
     3301 ttcctgcagg gctcggggct ggccaccgac cagttcagcg tcgtcgtgta cgaaccggcc
     3361 cgcaaagtcg aggtcctctg cccgttcact gacgaccggg gggagatcac gcgggccctg
     3421 gaggcgagtc tcgaggaccc gaggcgcttc tccgactacg cgaccgagcg gg
//
LOCUS       DASRIQ010000487         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_207897, whole genome shotgun sequence.
ACCESSION   DASRIQ010000487 DASRIQ010000000
VERSION     DASRIQ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207897"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFD06_11850"
     CDS             complement(<1..654)
                     /locus_tag="VFD06_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZN04265.1"
                     /translation="MDRIEAFLEHLRLGRNASPMTVRCYRTDLREFESFLRTGLRGYR
                     RGGTFSDAPAPGRAPALTAVDALMVRAYLAFLSERGVSRATVARKLAALRSFYREMER
                     QGVMTINPAALVATPRQDKRLPKPMTQQDVTVLLDGAFGPGPRDARDRALLEMLYATG
                     CRVSEIVGADLGDLELPPGAARRRAARAGAVSADAEGMLRVLGKGSKERLVPVGSKAV
                     "
     gene            909..1181
                     /locus_tag="VFD06_11855"
     CDS             909..1181
                     /locus_tag="VFD06_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04266.1"
                     /translation="MKKNTQASIVAGVAVLALFAAVACGGQTKAPETPATQTTETAPA
                     APAAAPDGAAAPTDAAAPAAAPAGEAAPAGGAAPAGDAAAPAGGGR"
     gene            1401..2387
                     /locus_tag="VFD06_11860"
     CDS             1401..2387
                     /locus_tag="VFD06_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HZN04267.1"
                     /translation="MSHDSVESPPRLLERLRYEVRVRHYSRRTEEAYSAWVRRFILFH
                     GKRHPKTMGEPEVARFLTWLATERRVSANTQSQALSAILFLYREVLDADVGWVQNVVR
                     AKPSRRLPVVLTRDEVVVVLRHLSGTPWLVAALLYGSGLRLLEALRLRVKDVEPGALQ
                     LTVRSGKGQKDRITMLPRTLKEPLRRHLEIVRLQHAQDVARGGGRVELPDAIARKYPD
                     AGRDWGWQWVFPATRTYVDPASGERRRHHLHESVLQRAVHSAVRHSRLAKPASCHTFR
                     HSFATHLLEDGYDIRTVQELLGHGDVATTMIYTHVLNRGGRGVLSPADKLLS"
BASE COUNT          429 a         1105 c          912 g          450 t
ORIGIN      
        1 gaccgccttg ctccccaccg gcaccagccg ctccttgctc cccttcccca gcacgcgcag
       61 catcccctcg gcgtcggcgc tcaccgcgcc ggcgcgcgcc gcgcgacggc gggcggcgcc
      121 cggcggcagc tcgagatcgc cgagatcggc gccgacgatc tcgctgacgc ggcagccggt
      181 ggcgtagagc atctcgagga gcgcccggtc gcgcgcatcg cgcggtcccg gcccgaacgc
      241 accatcgagg aggacggtga catcctgctg cgtcatcggc ttcgggaggc gcttgtcctg
      301 ccgcggcgtc gccacgagcg ccgccgggtt gatcgtcatc accccctgcc gctccatctc
      361 gcggtagaag gagcggagcg cagccagctt gcgcgccacg gtggcgcgac tgacgccgcg
      421 ctcgctgagg aaggcgaggt aggcgcgcac catcagcgcg tccaccgcgg tcagcgccgg
      481 cgcgcgcccc ggcgccggcg cgtcggagaa ggttccgccg cgccgatagc cgcgcagccc
      541 cgtgcgcagg aacgactcga actcgcgcag gtcggtgcga taacaacgca ccgtcatcgg
      601 cgaagcgttc cgcccgagcc gcagatgctc cagaaacgcc tcgatccgat ccacgcgccg
      661 cattctagcg cgccacgcga caccatccgc gtccgcttta cgcggcccgc cgccatccgc
      721 gtccgattca atgcggcgcg ccgccgtcca ggtccgattc acaccacgcg gcgcactcca
      781 gaaatccgat tttccgtttg acagcgcgcg atccttcgcc taccatcccc ggattgcgtt
      841 ttccggcacg attccggcat acgccggacc atcattcaac ggctcatcgt tcacatcgga
      901 gggatttcat gaagaagaac acgcaagctt cgatcgtcgc cggcgtggcc gttctggccc
      961 tgttcgccgc cgtcgcctgc ggcggccaga ccaaggcccc cgagaccccg gccacccaga
     1021 cgaccgagac cgctccggcc gctccggctg ctgctcccga tggcgcggcc gctccgaccg
     1081 acgccgccgc gcccgcggcc gccccggcgg gcgaagccgc tccggccggt ggcgccgctc
     1141 ctgccggcga cgccgcggct cccgctggcg gcggccgtta agccgatcgt cacgtcgtaa
     1201 gcattcaatg tggggcgggg agcgatcccc gccccacttg ctttatcatt cggttcgcag
     1261 tctccattag gtcgcctttc ccgcccccga cgttccgcgc ctccgccgtc cccgcacctc
     1321 cgccgtcccc acagttgaga cgaccgtcag tcgcagtcct cgcgtcgccc ctgaaccacg
     1381 tcgccccgga aaggaccgtc gtgagtcacg acagcgtcga gtcgccgccg cgcctgctcg
     1441 agcggctgcg atacgaggtg cgggtgcgcc attacagccg ccgcactgaa gaggcgtact
     1501 ccgcctgggt gcgtcgcttc atcctgttcc acggcaagcg gcatccgaag acgatggggg
     1561 agccggaggt ggcgcgcttc ctgacctggc tcgcgaccga gcgccgcgtc agcgccaaca
     1621 cccagagcca ggcgctgagc gccatcctct tcctctatcg agaggtgctc gacgcggacg
     1681 tcggctgggt ccagaacgtc gtgcgcgcca agccgtcccg ccgcctgccg gtcgtcctga
     1741 cgcgcgacga agtggtcgtc gttctacggc acctgtccgg aacaccgtgg ctggtcgccg
     1801 cgctcctgta cggctcggga ctgcggctgc tcgaagcact gcgtctgcgc gtcaaggatg
     1861 tcgagcccgg cgcgctccag ctgaccgtcc gctcgggcaa agggcagaag gatcgcatca
     1921 cgatgctacc gcggactttg aaggagccgc tgcgccgcca cctcgagatc gtccgcctgc
     1981 agcacgccca ggacgtggcg cgcggcggcg gccgggtcga gttgccggac gcgatcgccc
     2041 gcaagtaccc tgacgccggc cgggactggg gctggcagtg ggtcttcccc gcaacgcgga
     2101 cttacgtgga cccggcttcc ggcgagcggc gccgccacca ccttcacgaa tcggtcctgc
     2161 agcgcgccgt tcacagcgcg gttcgccact cccggctcgc caagccggcc tcgtgccaca
     2221 ccttccgcca ttccttcgcc acgcaccttc tggaggacgg ctacgacatc cgcaccgtcc
     2281 aggagctcct cggccatggc gacgtcgcga cgacgatgat ctacacccac gtcctcaacc
     2341 gcggaggccg cggcgtcctc agccccgccg acaagcttct gtcatgactc cccgcggacg
     2401 ccgaacctcg ccacacaatc ttgcgtggtt ttatccccgt atccgtctcc cccccgtcag
     2461 cctatttcat caaagccctc cccgcccttc ggtgctaacc tatgccccat cagcggccta
     2521 cggccagccc tccatgccgc ccaccgcatt agctgaccgt agccccgcag ccctattttt
     2581 ttaccgaggt ctgtctactt agcgttagcg tcgccacgaa aggatgtggg ttgtgttgtc
     2641 ggaagtcgtc gaagcgtgcg gggtgaagga gcagccgcgg cttcggggtc gcaaggctgc
     2701 aagcgtggta gtggccagcg ccgcgatcca gatgaacaac acgaggtgcc acgcgggcac
     2761 gtgcctgagc ccccgcggcg tgccagtcga gatggtcgtt gaacgaggca tcgctgcacg
     2821 ccgacggggt gagtcccgct tcggggtgcg cgctaacatc tgcgtgcagc ggtcggcggg
     2881 ccgcgtgccc gcgaga
//
LOCUS       DASRIQ010000488         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_727362, whole genome shotgun sequence.
ACCESSION   DASRIQ010000488 DASRIQ010000000
VERSION     DASRIQ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727362"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFD06_11865"
     CDS             complement(<1..208)
                     /locus_tag="VFD06_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN04268.1"
                     /translation="MPTILIADDEKNIRATLARALRLEGYATEEAEDGRKALERLESG
                     EVDLLILDLQMPVLDGLGLLEEISG"
     gene            complement(220..2100)
                     /locus_tag="VFD06_11870"
     CDS             complement(220..2100)
                     /locus_tag="VFD06_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN04269.1"
                     /translation="MSLRLRLALALTFVALLPMMVVAGRPLLNAQRNADEEGARRLDA
                     ARRQAAVLIRREQADLRGRVERAASDLAADRSAPRALQQGPASTVRALARLLGERHGL
                     DHLELRGPGESVLAVFDPLGSGPVVLPGSLAPDDVAIADLPLAALPPPRDIEPEPETG
                     DDAPPRPLPANPPEHGMAAIRSVQAGGEQYEILGVRRLGAPFLDAVAEVTGAPAALVA
                     PDGRTLDNGTATDPGSGPPRDAGMPLIADVPLAPGSWSVRVAAAPGDAAAVRRDLLAS
                     LVSVAPFALLAAALLGILLAEGISRPVRALAARAESISAERAGEMLTPLTRDGDEVRG
                     LTRSFDRMLEALSQSEVKRLSAERIAAWQEVARRVAHEVRNALSPIRLAVENLRRTRQ
                     RAPEEIDRALGEEGTAILEEVESLRRLVEEFSLFARLPAPQCAPCDLTEVARQSLALA
                     APRIAAMGVTVDLEDGGRPHVVHADAGFLGRALGNVVANALDAMERSPEKRLRIALRT
                     APGGTPAAATPPGVPGIEEIEVRDSGPGFAAEVAERMFEPYVTTRSERGGTGLGLTLV
                     QRVVADHGGSVSAGTAPGGGAFVLIRLPVAGPPASGAKTGASPAAADVPRSASPRHLR
                     SA"
     gene            complement(2121..>2618)
                     /locus_tag="VFD06_11875"
     CDS             complement(2121..>2618)
                     /locus_tag="VFD06_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04270.1"
                     /translation="TIEDHRRSGRFQKHYRTLQAGSEEDDPESGYSIGTLLRLSEYRY
                     AGRETIDGTECHRLDFDPQEKPPGNGIEARIAEAMSGSLWLTADGAHLVRARTTSTRP
                     VSLSLGLFKVHALEVAFDAHQVASGTWLPRQLSIVSHARLVFLGQHRRRVFTYSNYAP
                     LPRPG"
BASE COUNT          337 a          986 c          957 g          338 t
ORIGIN      
        1 gcccggagat ctcctcgagc agcccgagcc cgtcgagcac cggcatctgc agatcgagga
       61 tgagcaggtc gacctccccg gattccagcc gctcgagcgc cttgcggccg tcctccgcct
      121 cctcggtcgc gtacccttcg aggcggagcg cgcgggccag cgtcgcccgg atgttcttct
      181 cgtcgtcggc gatgaggatg gtcggcatgg gctgggctcc taggcgctcc ggagatgtcg
      241 gggagaggcg gagcgcggaa cgtcggcggc cgcgggcgac gctccggtct tcgccccgga
      301 cgcgggcggt cccgcgaccg gcaggcggat gaggacgaat gcgccgcccc ccggcgccgt
      361 gccggcgctc acgcttccgc cgtggtcggc gaccaccctc tggacgaggg tgagaccgag
      421 accggtgccg ccgcgctcgc tccgggtcgt gacgtacggc tcgaacatcc gctccgccac
      481 ctcggcggcg aagccgggac cgctgtcccg cacctcgatc tcctcgatcc cgggaacgcc
      541 cggcggcgtc gccgcggccg gcgttccgcc cggtgctgtg cgcagggcga tcctcaggcg
      601 cttctccggc gaccgctcca tcgcatcgag cgcgttggcg acgacgttgc cgagcgcccg
      661 ccccaggaac ccggcgtcgg cgtgcaccac gtggggacgc ccgccgtcct cgagatctac
      721 cgtcacgccc atcgccgcga tgcgcggcgc cgccagcgcc agcgattgcc gcgcgacctc
      781 cgtcagatcg cacggcgcgc actgcggcgc cggcaggcgc gcgaagaggg agaactcctc
      841 caccaggcgt cgcaggctct cgacctcctc gaggatcgcc gtcccctcct cgccgagggc
      901 gcggtcgatc tcctccggcg cccgctggcg ggtgcggcgc aggttctcca ccgcgagacg
      961 gatgggggac agcgcattcc gcacctcgtg cgccacccgg cgcgccacct cctgccaggc
     1021 ggcgatccgc tccgccgaca ggcgcttgac ctcgctctgc gacagcgcct cgagcatgcg
     1081 atcgaacgac cgggtcagac cgcgcacctc gtcgccgtct ctggtgagcg gggtcaacat
     1141 ttctccggcc cgctcggcgg agatcgattc ggcccgcgcc gccagggccc ggaccggccg
     1201 ggagatcccc tccgcgagga ggatgccgag cagggctgcc gcgagcagcg cgaacggcgc
     1261 cacgctcacg agcgacgcga gcagatcgcg gcgtaccgcg gcggcgtccc ccggcgccgc
     1321 ggcgacccgc acgctccagc tgcccggcgc gagcgggaca tccgcgatga ggggcatccc
     1381 cgcgtcccgc ggagggccgc tgcccgggtc cgtcgctgtc ccgttgtcga gagtcctgcc
     1441 gtccggagcg acgagcgcgg ccggcgcacc ggtcacctct gcgaccgcat cgagaaaggg
     1501 cgcgccgagg cgccggaccc cgaggatctc atactgctct ccgccggcct gcacgctgcg
     1561 gatcgcggcc attccgtgct ccggggggtt cgcaggcagc ggccgcggcg gcgcgtcgtc
     1621 gccggtttcg ggttccggct cgatgtcccg cggcggcggc agcgccgcga gcgggaggtc
     1681 ggcgatcgcg acgtcgtccg gagcgaggga gcccgggagg acgaccgggc cagagccgag
     1741 cggatcgaag accgccagca ccgattcgcc cgggccgcgc agctcgaggt ggtcgaggcc
     1801 gtgccgctcg ccgaggagcc gcgccagggc ccgcacggtg gaggcgggcc cctgctgcag
     1861 cgcccgaggc gccgaccggt cggcggccag gtcggaggcg gcccgctcca cccggccgcg
     1921 caggtccgcc tgctcgcggc ggatcaggac cgccgcctgg cggcgcgccg cgtcgagccg
     1981 ccgcgccccc tcctcgtcgg cgttgcgctg cgcgttcagg agcgggcgcc cggccaccac
     2041 catcatcggc aggagggcca cgaaggtcag cgccagggcc agtcgcagcc ggaggctcat
     2101 cgtggcggaa ctctaaggcg tcaccccggc ctgggcaagg gcgcgtagtt ggagtaggtg
     2161 aagacgcgcc gccggtgctg tccgaggaac accaggcgcg catggctcac gatcgacagc
     2221 tgccggggca gccaggtccc ggaagcaact tgatgggcgt cgaacgccac ctcgagcgcg
     2281 tgcaccttga agaggccgag cgagagcgaa accggccgcg tgctcgtcgt cctggcgcgg
     2341 acgaggtgcg ccccgtcggc ggtcagccac aggcttccgg acatcgcctc ggcgatgcgc
     2401 gcctcgatgc cgttcccggg cggcttctcc tggggatcga aatcgaggcg atggcactcg
     2461 gtcccgtcga tcgtctcgcg cccggcatag cggtactccg aaagccgcag gagcgtgccg
     2521 attgagtagc cgctctccgg atcgtcctcc tcgctccccg cctggagcgt gcggtagtgc
     2581 ttctggaagc ggccgctccg gcggtggtcc tcgatggt
//
LOCUS       DASRIQ010000489         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1247406, whole genome shotgun sequence.
ACCESSION   DASRIQ010000489 DASRIQ010000000
VERSION     DASRIQ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247406"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFD06_11880"
     CDS             <1..494
                     /locus_tag="VFD06_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HZN04271.1"
                     /translation="RLDPDWTTVGGWPGIHFGTSASGAGDVNGDGYSDIVVGAPEFSD
                     GEAAEGRALLYYGHGFCEAPDLPVFIAGARHVPGTNGVVLDFEDPNPTGTVTGYIVYR
                     AADPALDPALWPRIAFGIGDADPAAPGLQWEDETGADPTPGGAWFYQITPFDAVCAAE
                     GPR"
     gene            complement(523..1122)
                     /locus_tag="VFD06_11885"
     CDS             complement(523..1122)
                     /locus_tag="VFD06_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04272.1"
                     /translation="MKRAFFFVALLLGACRGAENASAPPPTATRPAPIDDEALMNGVY
                     CSSDIVTEGPVRLANGQWSAEDDEGRQTLELGKDRARGDLDGDGREDAAVVLMGSGGG
                     SGVFVDLALVLDRNGVPLHAASVALGDRVQVHGVSIEGNEVIVDVTQHGPGDPVCCPT
                     QRVTRRFRYADGRLTDTAPPPPSEGPPVGEASGTAAPSS"
     gene            1173..>2217
                     /locus_tag="VFD06_11890"
     CDS             1173..>2217
                     /locus_tag="VFD06_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HZN04273.1"
                     /translation="MQKASWPDRGRLASALRRETERFAETHPRSRALHARARKSLLAG
                     VPMHWMVRWPGGFPIVVTEGSGGRFRDVDGHEYVDFCLGDTGAMTGHAPAAVTEAVAE
                     RMRRGATFMLPVEDALVVAEELQRRFGLPFWQYAVSATDANRFAIRIARHLTGRSRIL
                     VFNWCYHGTVDEAFITLGDDGRASTRPGNIGPPVDPTVTTRVVEFNDLPALEAALATS
                     DVACVLAEPAMTNIGIILPEPGFHRALRDLTRRAGTLLILDETHTLSAGPGGCTRAWG
                     LEPDILTIGKAIAGGIPAAAYGLSDAVAEGIDPRIHEDESDAGGIGGTLAGNAVSIAA
                     MRATLESLLTEERF"
BASE COUNT          313 a          782 c          810 g          312 t
ORIGIN      
        1 cgcggctcga tccagactgg acgacggtcg gaggctggcc ggggatccac ttcgggacga
       61 gcgccagcgg ggccggcgac gtgaatggcg acggctactc cgacatcgtc gtcggggcgc
      121 ccgaattcag cgacggcgaa gcggccgagg gtcgggccct tctctactat ggccacggat
      181 tctgcgaggc gccggatctt ccggtgttca tcgccggggc gcggcacgtc cccgggacga
      241 acggagtcgt gctcgatttc gaggatccga atcccacggg gacggtcacg ggatacatcg
      301 tctaccgcgc ggccgatccg gctctcgatc cggctctgtg gccgcggatc gccttcggga
      361 tcggcgatgc cgatccggca gcgccggggc tgcagtggga ggacgagacc ggtgccgatc
      421 cgacgcctgg aggcgcctgg ttctaccaga tcaccccctt cgacgccgtc tgcgcggccg
      481 aggggccgcg ctaggccccc agccccgacc tccagggggg gactacgacg aaggggcggc
      541 ggtccctgac gcctccccca cgggaggacc ctcggagggg ggcggcggcg cggtgtcggt
      601 gagccggccg tcggcgtacc ggaagcggcg ggtgacgcgc tgcgtcgggc agcagacggg
      661 atcccccggc ccgtgctgcg tcacgtccac gatcacctcg ttcccttcga tggagacgcc
      721 gtgcacctgg acgcggtcgc cgagcgcgac cgaggcggcg tgcagcggca ctccgttgcg
      781 atcgaggacg agcgccagat cgacgaagac gccgctgcct ccccccgatc ccatgagaac
      841 gacggcggcg tcctcgcggc cgtcgccgtc gagatcgccg cgcgcccggt ccttccccag
      901 ctcgagggtc tggcgtccct cgtcgtcctc ggcgctccat tgcccgttcg cgaggcgcac
      961 cggcccctcg gtgacgatgt ccgaggagca gtagacgccg ttcatcagcg cctcgtcgtc
     1021 gatcggagcc ggtctcgtgg cggtcggtgg gggggccgag gcgttctccg cgccccggca
     1081 ggcgccgagg agcagggcca caaagaaaaa cgcgcgtttc atctcgaggg ccggccgaga
     1141 atagcacgcc cggagtgtgc tagaggtgac ccatgcagaa agcctcctgg cccgatcgcg
     1201 ggcggctcgc gtcggccctg cggcgcgaga ccgagcgctt cgccgagacg cacccgcgat
     1261 cccgcgcctt gcacgcccgg gcgcggaagt cgcttctcgc cggggtgccg atgcactgga
     1321 tggtgcgctg gcccggcggc ttcccgatcg tcgtcaccga gggatcgggg gggcggttcc
     1381 gggacgtcga cggccacgag tacgtcgact tctgcctcgg ggacactggc gccatgacgg
     1441 gccacgcccc cgcggcggtg accgaggcgg tggcggagcg gatgcggcgc ggtgccacct
     1501 tcatgctgcc ggtcgaggat gcgctcgtgg tcgccgagga gctgcagcgg cgcttcgggc
     1561 ttcccttctg gcagtatgcg gtcagcgcca ccgacgcgaa ccgcttcgcc atccgcatcg
     1621 cccggcacct gaccggccgc tccaggatcc tggtcttcaa ctggtgctac cacggcacgg
     1681 tggacgaggc gttcatcacg ctcggcgacg acggacgcgc ctccacccgg ccggggaaca
     1741 tcggacctcc cgtggatccg acggtgacga cgcgcgtggt cgagttcaac gacctcccgg
     1801 ccctcgaagc ggccctcgcc acgagcgacg tcgcctgcgt cctcgccgag ccggcgatga
     1861 cgaacatcgg catcatcctg ccggagcccg gcttccaccg tgcacttcgc gatctcacgc
     1921 ggcgcgccgg gacgctgctg atcctggacg agacgcacac cctgagcgcg ggaccgggag
     1981 gctgcacccg cgcctggggg ctggagccgg acatcctgac gatcggcaag gcgatcgccg
     2041 gcgggattcc ggcggcggcg tacggactgt cggacgcggt ggcggagggg atcgacccgc
     2101 gtatccacga agacgagtcg gatgccggcg ggatcggcgg caccctcgcc gggaacgccg
     2161 tgtcgatcgc cgcgatgcgg gcgaccctcg agtcgctcct gacggaagag cgcttca
//
LOCUS       DASRIQ010000490         6129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_208227, whole genome shotgun sequence.
ACCESSION   DASRIQ010000490 DASRIQ010000000
VERSION     DASRIQ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208227"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VFD06_11895"
     CDS             <1..1266
                     /locus_tag="VFD06_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN04274.1"
                     /translation="LLSSNPPITVPAWACMMTSQDAGQLGIYGFRNRKDHSYVGSSIA
                     NAAALQKQVAWDHLGAAGKQVIVLGVPPSYPPRPLNGVQVGCFLTPTTRRPYTHPPDL
                     KDEIDRTVPGYVVDVEDFRTDDKEGVLQRIYEKSSRHFALAKHLLKTRPWDFFMMVEM
                     GTDRIHHAFWAHMSKEHPKHEPGNRFEHAIRDFYKYCDSEVGELLALLPKDTAVMVVS
                     DHGARDLTGGICFNEWLIEKGDLVLKSYPEKPTPIAQCEIDWEKTKAWGDGGYYGRLF
                     MNVRGREPQGCVDPADYEKVRSELIAGIEAIADPQGKNIGSRAYRPQDIYREVRNVAP
                     DLMVYFGNLDWRSIGTVGTRSLYTFDNDTGPDDANHDWNGIFIMRTPDGTPQPKGEQQ
                     GLRLYDIAPTVLRLSGLTPPDGMIGRSIV"
     gene            1294..1815
                     /locus_tag="VFD06_11900"
     CDS             1294..1815
                     /locus_tag="VFD06_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN04275.1"
                     /translation="MRDMNLRDTERDAVLLALRDMPSWLEGAVRDLSPDEEVLRGPGG
                     GFAPVEQCWHLAELERDAFSERLRRLREEDRPVLPDFDGERAAIDGNYLGRTCADGVA
                     AFAAARRANLDLIGSIRAEEWGRTGTQAGVGELALCDLPAMMAEHDASHRREIETWLG
                     DRGKRPGGGGTGR"
     gene            1812..2210
                     /locus_tag="VFD06_11905"
     CDS             1812..2210
                     /locus_tag="VFD06_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04276.1"
                     /translation="MKSYIRSVAGVVFGACVAIGVIILVEQVSGLMDPPPAGMDFGDP
                     EVFKAFVAGLPVGAFLMILVAWGLGTFAGAWVCARIAGRAKLTHGVVLGALLLVAGVT
                     NVLMIPHPAWMWGSAFLVFAGGGYAGGRLA"
     gene            2294..2980
                     /locus_tag="VFD06_11910"
     CDS             2294..2980
                     /locus_tag="VFD06_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN04277.1"
                     /translation="MLTLEKVDKSYVSGRNRLHVLKAVDLTIEQGEMVSIMGASGSGK
                     STLMNILGLLDTYDSGAYTFDGTLVRGLSETRSAVLRRQWVGFVFQSFNLIPFKTAAE
                     NVALPLYYQGIGRRRRLDMAKEHLRRVGLEDRAEHRPSELSGGQQQRVAIARALVSKP
                     KVLLADEPTGALDSSTSGEIMRLLQEVNAEGVTVVVVTHERDVAAATRRIIRLKDGRI
                     EDANARLPEA"
     gene            2982..4214
                     /locus_tag="VFD06_11915"
     CDS             2982..4214
                     /locus_tag="VFD06_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04278.1"
                     /translation="MFDLDTWQEIVDTIRSNKLRSFLTAFSVAWGIFMLIILLGSGQG
                     LAHGVEYQFRDDAINSIWVIPGQTSMPYKGLQPGRRVQLTNEDHDDIERGVDGVEHIT
                     SRFMIAGTLRVRYRGETGTFDVRAVHPGHLFVEKTIITDGRFLNELDIREHRKVVVLG
                     SKVAQALFKGGPALEKEIEINGIAFKVVGLFTDVGGEGELEKIYLPVSTAQRAFGGAN
                     RIGQIMFTTGEAPIDKTKAMSGDVHERLAQRHTFSMEDARAVYVRNNNENFQRFIQVT
                     TAIRLFVWVVGLGTILAGVVGVSNIMMIAVRERTREIGVRRALGATPWSVVSLILKES
                     ILITSVAGYVGLVLGLVVLQIGASFLPKDGFFINPEVDLSVALQATILLIVAGAAAGY
                     FPARRAGRLRPVEALRSE"
     gene            4255..5517
                     /locus_tag="VFD06_11920"
     CDS             4255..5517
                     /locus_tag="VFD06_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04279.1"
                     /translation="MSVLDRDHWEEILQTLARNKMRTFLTAFGVFWGIFMLMIMLGSG
                     NGLSNGIESGFADGATNSFFIWSQRPSKPYRGMQPGRELHLDLADVAAIREQVPEAAV
                     ICPRNQLGGWRGGNNITRGNKAGAFSVMGDFPEIAQVQSVRMVAGRFLNQLDVAERRK
                     VAVLGPRSREVLFEKGEDPLGQDIRINGVYFKVIGVFQPRRSGDDHDDDAMSLFVPFT
                     TFQSAFNFGDKVGWFAITSKEGVPASIPEEKTLALLRERHRVAPDDTRAFGHFNVEKE
                     FGQIRGLFAGIRMLIWIVGTGTLAAGVIGVSNIMLVIVRERTNEIGVRRAVGATPWAI
                     MTQVVFESLVLTSTAGYLGLVAGVAVIEMTSRLVGDGAEMFKNPEVSLGAALQTLTIL
                     VLSGLLAGLFPARRAVQSTPVEALRAIV"
     gene            5522..>6129
                     /locus_tag="VFD06_11925"
     CDS             5522..>6129
                     /locus_tag="VFD06_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZN04280.1"
                     /translation="MKRVLTVLAVVGLVGLFGGTLFFLWSKSQKKPVVYRTETPEMSD
                     IVKKAVATGAVVPRKEVAVKPQISGIIETIKVEPGQRVRRGDLLATIRVIPQTAALAV
                     AESRVNQARLKLDDAERELVRNEKVYREGILPETTLRQVILARDTANEELAGAIDTLE
                     VIRKGVPARSREGTNTLVKATIEGTVLDVPVKEGTSVIEANT"
BASE COUNT          993 a         2035 c         2159 g          942 t
ORIGIN      
        1 ctcctcagca gcaacccgcc gatcaccgtc cccgcctggg cctgcatgat gacgagccag
       61 gatgcggggc agctggggat ctacggtttc cgcaatcgca aggaccactc gtacgtcggt
      121 tcctccatcg ccaacgccgc ggcgctgcag aagcaggtcg cctgggatca cctcggggcc
      181 gcggggaagc aggtgatcgt cctcggcgtg ccgccgtcct acccgccgcg cccgctcaac
      241 ggagtgcagg tgggttgctt cctgaccccg acgacgcgcc ggccatacac gcacccgccc
      301 gacctcaagg acgagatcga tcgcaccgtc cccggatacg tcgtggacgt cgaggatttc
      361 aggacggacg acaaggaagg cgtgctgcag cggatctacg agaaatcgag ccggcacttc
      421 gccctggcga agcaccttct caagacccgg ccgtgggact tcttcatgat ggtggagatg
      481 gggaccgacc ggatccacca cgccttctgg gcgcacatgt cgaaggagca cccgaagcac
      541 gagccgggga accgcttcga gcatgccatc cgcgacttct acaagtactg cgacagcgag
      601 gtcggcgagc tcctggcgct cctgccgaaa gacaccgcgg tcatggtcgt ctccgatcac
      661 ggggcgcgcg acctcacggg cggcatctgc ttcaacgagt ggctcatcga gaagggggac
      721 ctggtgctca agtcgtaccc tgagaagccg acgccgatcg cccagtgcga gatcgactgg
      781 gagaagacga aggcctgggg ggacggcggt tactacggcc gcctcttcat gaacgtgcgg
      841 gggcgggagc cgcagggatg cgtcgatccg gccgactacg agaaggtccg atccgagctc
      901 atcgccggca tcgaggcgat cgccgacccg caggggaaga acatcggctc acgcgcctat
      961 cgcccccagg acatctaccg cgaggtcagg aacgtggcgc cggacctgat ggtctacttc
     1021 ggcaacctcg actggcgatc gatcggcacg gtcggcacgc gctccctcta caccttcgac
     1081 aacgacaccg ggccggacga cgccaaccac gactggaacg gcatcttcat catgaggacc
     1141 ccggacggca cgccgcagcc gaagggggag cagcagggcc tgaggctgta cgatatcgcc
     1201 ccgacggtct tgcgcctctc gggcctcacg ccgcccgacg ggatgatcgg ccgctcgatc
     1261 gtctgacacg agccaggctc ccagggaggc cccatgcgcg acatgaatct ccgggacacg
     1321 gagcgggacg cagttctgct ggccttgcgc gacatgccgt cctggctcga gggggcggtg
     1381 cgggacctgt cccccgacga ggaggtgctt cgcggccccg gcggcggctt cgcgccggtc
     1441 gagcagtgct ggcacctggc ggagctggag cgggacgcct tctccgagag gttgcgccgg
     1501 ctgcgcgagg aggaccgtcc ggtcctgccg gatttcgacg gcgagcgggc cgccatcgac
     1561 gggaactatc tcggccggac ctgcgcggat ggtgtcgccg ccttcgccgc ggcgcgccgg
     1621 gccaacctcg atctcatcgg ctcgatccgc gccgaagaat gggggcggac cgggacccag
     1681 gccggggtgg gtgagttggc gctgtgcgac ctgccggcca tgatggcgga gcacgatgcc
     1741 tcgcaccgcc gcgaaatcga gacctggctc ggagatcgcg ggaaacggcc cgggggcggc
     1801 gggaccggcc gatgaagtca tacatcagga gcgtcgccgg cgtggtcttc ggcgcctgcg
     1861 tcgccatcgg cgtcatcatc ctcgtcgagc aggtgagcgg cctgatggac ccgccccccg
     1921 ccgggatgga cttcggcgat cccgaggtgt tcaaggcgtt cgtcgccggt ctcccggtcg
     1981 gggcgttcct gatgatcctg gtcgcctggg ggctgggcac cttcgccggc gcctgggtct
     2041 gcgcccggat cgcgggacgg gcgaagctga cccacggggt cgtcctcggg gcgctcctgc
     2101 tcgtcgcggg ggtgacgaac gtgctgatga ttccgcaccc ggcgtggatg tggggctcgg
     2161 ccttcctggt gttcgccggc ggagggtacg ctggcggccg cctggcctga gggacccctc
     2221 ccggggccgt cccgaatccg aacgctcgcc tggaaccccc tgtccgggct atcgttctac
     2281 ctctctgaac cccatgttga ccctcgagaa ggtggacaag tcgtacgtct cgggcaggaa
     2341 ccgcctgcac gtcctcaagg cggtcgacct gaccatcgag cagggggaga tggtgtcgat
     2401 catgggggcc tccggctcgg gaaaatccac gctgatgaac atcctgggcc tcctcgacac
     2461 ctacgactcc ggggcctaca ccttcgacgg gacgctggtg agagggctgt cggagaccag
     2521 gtccgcggtg ctgcggcgcc agtgggtcgg gttcgtgttc cagtcgttca acctgatccc
     2581 gttcaagacc gcggcggaga acgtcgcgct gccgctctac taccagggga tcggccggcg
     2641 tcggcggctg gacatggcga aggagcacct gcgccgcgtc gggctcgagg accgcgccga
     2701 gcaccgcccc tcggagttgt cggggggaca gcagcaacgg gtggcgatcg cccgcgcgct
     2761 cgtctcaaag cccaaggtcc tcctcgccga cgagccgacc ggggccctgg actcctccac
     2821 ctcgggtgag atcatgcgcc tcctccagga ggtgaacgcc gagggggtga cggtcgtcgt
     2881 cgtgacgcac gagcgggacg tggcggcggc gacccggcgc atcatccgcc tgaaggacgg
     2941 ccgcatcgag gacgccaacg cgcgcctccc cgaggcctga catgttcgat ctcgacacct
     3001 ggcaggagat cgtcgacacc atccgcagca acaagctgcg ctcgttcctc accgccttct
     3061 cggtcgcgtg ggggatcttc atgctcatca tcctgctcgg atcggggcag gggctggcgc
     3121 acggggtcga gtaccagttc cgcgacgacg ccatcaacag catctgggtg atccccggtc
     3181 agacgagcat gccgtacaag ggactgcagc cggggcggcg ggtccagctg acgaacgagg
     3241 accacgacga catcgagcgc ggggtggacg gcgtcgaaca catcacctca cgcttcatga
     3301 tcgccgggac gctgcgggtg cgctaccggg gcgagaccgg caccttcgac gtgcgggcgg
     3361 tgcatccggg gcacctcttc gtcgagaaga cgatcatcac ggacgggcgt ttcctgaacg
     3421 agctcgacat ccgcgagcac cgcaaggtgg tggtcctcgg atcgaaggtg gcgcaggcgc
     3481 tcttcaaggg gggaccggcg ctcgagaagg agatcgagat caacggcatc gccttcaagg
     3541 tcgtggggct gttcaccgac gtcggcggcg agggggagct ggagaagatc tacctgccgg
     3601 tctccacggc gcagcgggcc ttcggcggcg ccaaccgcat cgggcagatc atgttcacca
     3661 cgggcgaggc gccgatcgac aagaccaagg ccatgtcggg cgacgtgcac gagcggctgg
     3721 cgcagcggca caccttctcg atggaggacg cgcgcgccgt ctacgtccgc aacaacaacg
     3781 agaacttcca gcgcttcatc caggtgacga ccgccatccg cctgttcgtc tgggtcgtcg
     3841 ggctcggcac catcctggcc ggcgtggtcg gggtgagcaa catcatgatg atcgcggtgc
     3901 gcgagcggac gcgcgagatc ggcgtgcgcc gggcgctcgg cgccaccccc tggtcggtcg
     3961 tgtcgctcat cctcaaggag tcgatcctca tcacctcggt cgcaggctat gtcggtctcg
     4021 tcctcgggct cgtcgtgctg cagattggcg catcgttcct gccgaaggac gggttcttca
     4081 tcaatcccga ggtggacctg tccgtcgccc tgcaggcgac gatcctgttg atcgtcgccg
     4141 gcgccgccgc ggggtacttc ccggcgcgcc gcgccggccg tctgcgcccg gtcgaggcgc
     4201 tgaggagcga atgaccaacc cccgcgccgc gagggcgcgc agagagcgcc gccgatgagc
     4261 gtgctcgacc gcgatcactg ggaggagatc ctccagaccc tggcgcgtaa caagatgcgc
     4321 accttcctga ccgccttcgg ggtgttctgg gggatcttca tgctgatgat catgctcggc
     4381 tccggcaacg ggctgagcaa cggcatcgag tccggcttcg ccgacggcgc gaccaactcg
     4441 ttcttcatct ggtcgcagcg gccgagcaag ccgtaccgcg gcatgcagcc ggggcgcgag
     4501 ctgcacctcg acctcgccga tgtcgcggcg atccgggagc aggtgccgga ggcggcggtg
     4561 atctgcccgc gcaaccagct cgggggctgg cgtggcggca acaacatcac gcgggggaac
     4621 aaggccgggg ccttcagcgt catgggggac ttcccggaga tcgcccaggt gcagtcggtc
     4681 cgcatggtcg cggggcgctt cctgaaccag ctcgacgtcg ccgagcgccg caaggtggcg
     4741 gtcctcgggc cgcgctcccg cgaggtgctg ttcgagaaag gggaggaccc gctcgggcag
     4801 gacatccgga tcaacggcgt ctacttcaag gtgatcggcg tgttccagcc gcggcgttcc
     4861 ggggacgacc acgacgacga cgcgatgagc ctgttcgtgc cgttcaccac cttccagtcg
     4921 gcgttcaact tcggcgacaa ggtcggctgg ttcgccatca cctcgaagga gggggtgccc
     4981 gcctcgatcc cggaagagaa gacgctggcg ctcctgcgcg aacggcaccg ggtggcgccg
     5041 gacgacacac gcgccttcgg ccacttcaac gtcgagaagg agttcggcca gatccggggg
     5101 ctgttcgccg gcatccggat gctcatctgg atcgtcggca ccggcaccct cgcggccggg
     5161 gtcatcggcg tcagcaacat catgctggtg atcgtgcgcg agcggacgaa cgagatcggc
     5221 gtgcgccgcg ccgtcggcgc gacgccgtgg gcgatcatga cgcaggtggt gttcgagtcg
     5281 ctggtgctga cctcgaccgc cggctacctc ggcctggtcg ccggtgtggc ggtgatcgag
     5341 atgacgtcgc gcctggtggg ggacggtgcg gagatgttca agaacccgga ggtttcactg
     5401 ggggcggcgc tgcagaccct gacgatcctc gtcctgtccg gcctcctcgc cggactcttc
     5461 ccggcacggc gcgccgtcca gtcgactccg gtcgaggcgc tccgggcgat cgtgtgaggc
     5521 gatgaagcgc gtcctgaccg ttctcgcggt ggtgggcctg gtggggctgt tcggcggcac
     5581 tctcttcttc ctgtggtcga agtcgcagaa aaagccggtc gtctacagga cggagacccc
     5641 ggagatgtcc gacatcgtca agaaggcggt cgccaccggc gcggtcgtgc cgcgcaagga
     5701 ggtggcggtc aagccccaga tctccggcat catcgagacg atcaaggtcg agccggggca
     5761 gcgcgtccgc cgcggcgacc tcctggcgac catccgggtc atcccgcaga ccgccgcact
     5821 cgcggtcgcg gagagtcggg tcaaccaggc gcgcctcaag ctcgacgacg cggagcggga
     5881 gctggtgcgc aacgagaagg tctacaggga ggggatcctg cccgagacga cgctgcgcca
     5941 ggtgatcctc gcgcgcgaca ccgcgaacga ggagctggcc ggcgccatcg acacgctgga
     6001 ggtcatccgc aagggggtcc cggcccgctc gcgcgagggg acgaacacgc tggtcaaggc
     6061 caccatcgag ggcaccgtcc tcgacgtgcc ggtgaaggag gggacctcgg tcatcgaggc
     6121 gaacacctt
//
LOCUS       DASRIQ010000491         7915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1755778, whole genome shotgun sequence.
ACCESSION   DASRIQ010000491 DASRIQ010000000
VERSION     DASRIQ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755778"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(811..1419)
                     /locus_tag="VFD06_11930"
     CDS             complement(811..1419)
                     /locus_tag="VFD06_11930"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZN04281.1"
                     /translation="MRSVLEFLKTTLVGGLFFLLPLIVTLYVLGKGIQAARRLVAPLS
                     RLMPFESVVGVAADTLLAVVALLVVCFLAGLVARTGVGQAIADRAENMILKKIPGYTI
                     LRSMTSDGVLSSGVRMQTALAWIEESWVLAFMVERNPSGLVTVFVPSAPTPAAGTIYY
                     LTEDRVRVIDVPVRVAVKLIMQLGVGSAEALRGKFDPGPGPK"
     gene            complement(1469..2041)
                     /locus_tag="VFD06_11935"
     CDS             complement(1469..2041)
                     /locus_tag="VFD06_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZN04282.1"
                     /translation="MRRRPLLFAALVLGAVFALTLAAPAAQAQTVKSDYDKKADFTTY
                     KTFTFKKGTDAPTPFAQERIVGAIANQLKARGLTQSETADLLVYTHTQVSTEQRVDTT
                     GFGYGGYPGWGGWGGGFATSSAVVTEIPIGTVIVDLVDAKTNELVWRGIASDTLLTNP
                     TPEKSEKRINKAITKLFSKYPVEPIAEKKK"
     gene            complement(2087..3334)
                     /locus_tag="VFD06_11940"
     CDS             complement(2087..3334)
                     /locus_tag="VFD06_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN04283.1"
                     /translation="MKSHRPRRCAIDLYMRELDSCPPLTAAEEQELCLRMLRLRERLA
                     VLLGGLPEPQRRVLLGVKATDARLPFAMLESMVAALRHAAEDRTGTRLAVTARRAAVV
                     LGALTATRSTFVTRNLRLTFHFARRLAGRSTPLADLIQAGNVGLLEAVDRFDPRRGTR
                     FSTYAGMYITRSVFRTVPRLSQPVHVPEYQRRLKSRLRDSRRSLVHELGRMPTLEETA
                     ARAHIEAAKARKILQASLTILELDGPAPGLERGSLADTMPGDDGMSVQRRLIAGDLQR
                     LLDDLLPALDPRMRLVLRLRYGLDGEREHTLQEIGEVLRLSRERVRQLEEEAVETLRA
                     RLRRLALRGLPSVPGRPARRLRATSSRPPAAATSGLAVDDVPPGAARRLELDLPPARR
                     ALPSASPAAVGPPSKARPGPDLI"
     gene            3452..4144
                     /locus_tag="VFD06_11945"
     CDS             3452..4144
                     /locus_tag="VFD06_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN04284.1"
                     /translation="MRILIVEDEERVAQFLRRGFEAESITVDVAGDGNEAAVVAREND
                     YDAIVLDLMLPGKDGLEVLRDLRAAGKSAPVLILSARGAVEDRVRGLDLGADDYLPKP
                     FAFAELLARVRALLRRPGEAERSPALRADDLVLDRLTRKVTRGGREIELTNKEYQVLE
                     YLLRHKNRVLSRVLIIEHIWDMQFDGGTNIVDVVINRLRRKIDDGARHALIRTARGVG
                     YLLEEPRHDDPA"
     gene            4128..5558
                     /locus_tag="VFD06_11950"
     CDS             4128..5558
                     /locus_tag="VFD06_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN04285.1"
                     /translation="MTIRPRTWIPARFRTLRAKLVFWYSGILFLAFLVVGSSVYAYLS
                     YEGRRAIRRGLRTQTTWISNRITSLQQPGAAAEPLSHLPPEIRADLQKHLDEAADHYS
                     VLVRSKEGREFYAAGDRAAIDVTRAPAVTGRTVLASIDRGPEGLYHVASLSRETFEVH
                     VGLTDANVRRVLANTRAILLLMAPLMVLIASVGGWFLARSALRPIDQIVAIAARRNAR
                     NLDERMPEPDVDDEVGRLIRTLNDTADRMTAVYRRIEQFAANVAHELRTPLTILRGEA
                     ELALAEPRSREELQRLSQTYLEESVRLSGIVDDLLTLAHADPSRVQVERRPIDMEALV
                     EDLRDDAGILTHDKGLRVDLVENAPATILGDVARLRRLFRSLIANAVRYTDAGGFIGI
                     RSHREGNAVRVSIEDSGIGIPEESLPRIFDRFYRADPARARDSGGTGLGLSLARWVAE
                     AHGGTIAVESAVGRGSTFTVTLPLAG"
     gene            5770..6423
                     /locus_tag="VFD06_11955"
     CDS             5770..6423
                     /locus_tag="VFD06_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04286.1"
                     /translation="MRRHLSVALLVCLFAAGASVAHAGTGGKPVTWQFDFGYSMVQGK
                     PSDVFEDGWTIGFGGVARPWPKTPLGIRWDFTYDWWDVNTGELQSGPGVIQIDDGDGD
                     QWSLRTGIQYESPGDKTKFIGGVGIGGYRVSADISNSVVVPGWICDPYYWWYCYPGLV
                     EGEVVIADKTLTKFGFYATAGMVFPLSSSDIYVELQYHWVNVEKYYETMPIVVGWRF"
     gene            complement(6487..7194)
                     /locus_tag="VFD06_11960"
     CDS             complement(6487..7194)
                     /locus_tag="VFD06_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04287.1"
                     /translation="MPRALFALSLSCLTLSPAFADTVDAAANPAAPASAASPSDSEAS
                     AQALRLAQQRLKLTPEQTEKVRPLMDAHVKKLRALFEEYTGGGAGVLPSFMQEFKQTR
                     ERFRDSLQPILTEPQKAELDVLRKEVDDSLRDLICDQRIAALKGPLALTPEQETALRP
                     ILKEDFEKKRAILAAHTGAAGGPGTRRWLGDEVKKVQAETEGRMAGVLNPGQLQTYRA
                     DLAKKTEAAKAAAPGKP"
     gene            7311..7814
                     /locus_tag="VFD06_11965"
     CDS             7311..7814
                     /locus_tag="VFD06_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04288.1"
                     /translation="MTSRPSPLFGLTLIAALFAAAPASAQTATDEKELTKSVITTERQ
                     AIVAANLNLTDEQGTVFWPLYKQYGAEMDAITDRKIRLIEDYARKYVGMTDEEAAGLM
                     SAYMAIQKDELKVRERWVVKMKKELPIKTVLRFFQIENKLDAIVRAETLRVIPLVRNQ
                     AMPTSNR"
BASE COUNT         1174 a         2744 c         2787 g         1210 t
ORIGIN      
        1 gcgtttcgtc gcggcgcggg ctgaccgccg gacctgccgg ggcgcaggtc tcggctcgtc
       61 cggcgaatcc gggccggccc accgacacgc cgtcggccgg cgcagcccgc gtcccgtcga
      121 accgcggggt cacggcacgc ccggcgacgc cgggccgccc gacctccggg aacgcctccc
      181 aggggcagac gaatcagaac ccgcgcgcga cgcgacccgg gcaggcagcc cgtcccgctc
      241 cctcgacacc gccggcaggc gccacgacgc aagggcagac acggccacgg caggcggcac
      301 ggccgcctca ggccggccgg ccgtcgaccc caggggcccc ctcgcagtcg cagcaggtcc
      361 gaccgtcgtc gccggggggc acctccacga agcaggcccg gccggcgaat ccgtcgcggc
      421 cgtcccagcc ggggcgtcct tctgcccggc cgtcgcggcc gggggctccg cctccccagg
      481 cttcgcgtcc ggcctcgccc caggcgtccc ggccttccgc cccgtcgcaa caggcgcggc
      541 cgtcttcgcc gcaagggcgc gtctactccc gtccgtcgca gccttcacgt ccctccgcct
      601 cgatgcgcgg tccttcgatc gggcgggcag gtggatccgg cggttacagc cgtccgtcga
      661 tgtcctcgat ggggggtggg cggtcgatgt cgtcagcgcc gcgcggtggc gggtacgccg
      721 gcggccgcgg cggcgggggt ggcggtggtc gtccgggggg cggcgcgcct cgcggcggtg
      781 gcggcggcgc ccgtcactga cgccctcaaa tcacttcggg ccggggcccg ggtcgaactt
      841 gccgcgcagc gcttcggccg agccgacgcc gagttgcatg atgagcttca ccgcgacccg
      901 caccggcacg tcgatcaccc gcacccgatc ctccgtcagg tagtagatcg tcccggccgc
      961 gggcgtcggc gcgctgggga cgaagacggt cacgagcccg ctgggattgc gctccaccat
     1021 gaacgcgagc acccaggatt cctcgatcca ggccagcgcc gtctgcatcc ggacgcctga
     1081 gctgaggacg ccgtcgctcg tcatgctgcg caggatggtg tatccgggga tcttcttgag
     1141 gatcatgttc tcggcccgat cggcgatggc ctggccgacc cccgtgcgcg cgacgagtcc
     1201 ggcgaggaag cagacgacga gcagcgccac caccgccagc agagtgtcgg ccgccacccc
     1261 aacgacgctt tcgaacggca tcagcctcga cagcggggcc acgagccgcc gggcggcctg
     1321 gatccccttg ccgagcacgt agagggtcac gatgagcggc aggaggaaga agagccctcc
     1381 caccagggtc gttttcagga attcgaggac cgagcgcatc catccaccgt cgcgctgcgc
     1441 cgtcgcggct acccgcagcg aggcccggct acttcttctt ctcggcgatc ggctcgaccg
     1501 ggtacttgga gaagagcttg gtgatcgcct tgttgatccg cttctcggac ttctccgggg
     1561 tcggattggt gagaagcgtg tcactcgcga tgccgcgcca caccagctcg ttggtcttgg
     1621 cgtcgacgag gtccacgatc accgtgccga tcgggatctc ggtcacgacc gccgacgagg
     1681 tcgcgaaccc gccgccccag cctccccagc cgggatagcc gccatagccg aagccggtcg
     1741 tatccacccg ctgctcggtg ctgacctggg tgtgcgtgta gacgagcagg tcggcggtct
     1801 cgctctgcgt caggccgcgg gccttgagct ggttcgcgat ggcgccgacg atccgctcct
     1861 gtgcgaacgg cgtcggcgcg tcggtcccct tcttgaaggt gaacgtcttg tacgtggtga
     1921 aatcggcctt cttgtcgtag tccgacttca cagtctgcgc ctgcgccgcc ggcgccgcga
     1981 gcgtgagggc gaacacggcg ccgagaacca gtgcggcgaa cagcaacgga cgtcgtctca
     2041 tcgttcgatc tccgctcgtg acgttgggga tggatgcgag cggattctag atcaagtcgg
     2101 gtccgggccg ggcctttgaa ggcggtccga cggcagccgg tgaagctgag ggaagggcac
     2161 ggcgcgccgg cgggaggtcc aattccaacc gacgcgccgc gcccggtggt acgtcatcga
     2221 ccgccagccc gcttgtcgca gcggcaggcg gccttgaact ggtcgcgcgg agccgacgag
     2281 caggccggcc tggcacggac ggcaggccgc ggagcgccag gcgccggagc ctggcgcgca
     2341 gcgtctcgac cgcctcctcc tcgagctgcc ggacgcgctc ccggctcagc cgaaggacct
     2401 cgccgatctc ctgcagggtg tgctcacgct cgccgtcgag cccgtaccgg aggcgaagca
     2461 ccaatcgcat gcggggatcg agcgccggaa gcaggtcgtc caacaggcgt tgcagatcgc
     2521 cggcgatgag ccgccgctgc accgacatgc cgtcgtcccc cggcatggtg tcggcgaggc
     2581 tgcccctctc caggccaggg gcggggccat ccagctccag aatcgtcagc gacgcttgca
     2641 ggatcttgcg ggccttggcc gcctcgatgt gcgcgcgggc ggccgtctcc tccagggtcg
     2701 gcatgcggcc cagctcatgg acgaggctgc ggcgcgagtc cctcaggcgg ctcttgagcc
     2761 gcctctggta ctcggggacg tgcaccggct ggctcaggcg cggcacggtc cggaacaccg
     2821 agcgggtgat gtacatcccg gcgtacgtcg agaagcgtgt cccccgccgc gggtcgaaac
     2881 ggtccaccgc ctcgaggagc cccacgttgc cggcctgaat gaggtcggcg agcggcgtgc
     2941 tgcgaccggc gagtcggcgg gcgaagtgga acgtcagccg gaggttccgg gtgacgaatg
     3001 tcgagcgggt ggccgtcagg gctcccagga ccaccgccgc ccggcgcgcg gtgaccgcga
     3061 gccgcgtccc ggtgcggtcc tccgctgcgt ggcggagcgc ggcgaccatc gattcgagca
     3121 tggcgaacgg cagacgcgcg tccgtcgcct tcacgcccag gaggacgcgg cgctgcggct
     3181 cggggaggcc gcccaggagc acggcgagcc gttcgcgcaa ccgcagcatc ctcaagcaca
     3241 gttcttgttc ctccgccgcg gtcagcggcg gacaggagtc gagttcccgc atgtagaggt
     3301 cgatcgcgca gcgacgtggc cggtggctct tcatggcctc tggataccgg aggctcatga
     3361 cggccacatg aaccgtgcat gaaatccctg tcatccgcga aaacaggccg atttgcgctg
     3421 aggggaagcc gggggctacc attccggcgt catgcgcatc ctcatcgtcg aggacgagga
     3481 acgggtcgct cagtttctcc ggcgcggatt cgaggcggag tcgatcaccg tggacgtcgc
     3541 cggcgacggg aacgaggccg cggtcgtggc ccgcgagaac gactacgacg ccatcgtcct
     3601 ggacctgatg ctccccggga aggacggcct cgaggtcctc agggacctgc gcgcggccgg
     3661 gaagtccgcg ccggtcctga tcctgtcggc ccgtggcgcc gtcgaggatc gcgtgcgcgg
     3721 tctcgatctc ggcgccgacg actacctgcc caagcccttc gccttcgccg agctcctggc
     3781 gcgggtgcgc gccctcctgc ggcgtcccgg cgaggcggag cggagccccg cgctacgggc
     3841 ggacgacctt gtgctcgatc ggctcacgcg caaggtgacg cgcggcgggc gggagatcga
     3901 gctcacgaac aaggagtacc aggtgctcga ataccttctg cgccacaaga accgggtcct
     3961 gtcgcgcgtc ctgatcatcg agcacatctg ggatatgcag ttcgacggtg ggaccaacat
     4021 cgtggacgtc gtcatcaacc gcctgcggcg caagatcgac gacggggcgc ggcatgcgct
     4081 gatccgcacg gcgcgcggtg tcggctatct cctggaggaa ccgcggcatg acgatccggc
     4141 ctaggacatg gatcccggcc cgcttccgga ccttgagggc gaaactcgtc ttctggtact
     4201 cgggcatcct gttcctggcg ttcctcgtcg tcggttcgag cgtgtacgcc tacctgagct
     4261 acgaggggcg gcgcgccatc cggcgtggtc tccggaccca gaccacctgg atctccaacc
     4321 gcatcacgag cctccagcag ccgggagccg cggccgagcc actctcgcac ctgccgccgg
     4381 agatccgcgc cgatctccag aagcatctcg acgaagcggc cgaccactat tccgtgctgg
     4441 tccgttcgaa ggaaggacgg gagttctatg ccgccgggga tcgcgccgcc atcgacgtca
     4501 cccgggcccc ggccgtcacc ggcaggaccg tgctcgcctc gatcgaccgc ggtccggagg
     4561 ggctctacca cgtggccagc ctgtcccggg agaccttcga ggtccacgtg gggctcacgg
     4621 acgcgaacgt gcgccgggtc ctcgccaaca cccgggcgat cctgctgctg atggcgcccc
     4681 tgatggtgct gatcgcgtcc gtcggtgggt ggttcctggc gcggtccgcc ctgcgcccga
     4741 tcgaccagat cgtcgccatc gccgcgcggc gcaacgcccg gaacctggac gagcggatgc
     4801 ccgaacccga cgtcgacgac gaggtcggcc gcctcatccg gacgctgaac gacaccgccg
     4861 accggatgac cgcggtgtat cgccgcatcg agcagttcgc cgcgaacgtc gcgcatgagc
     4921 tgcggacccc actgaccatc ctgcgcggtg aggcggagct ggcgctggcg gagccgcgca
     4981 gccgcgagga actgcagcgc ctgtcgcaga cctatctcga ggagagcgtg cggctgtcgg
     5041 ggatcgtcga cgacctgctc accctggcgc acgccgaccc gagccgggtg caggtcgagc
     5101 gccggccgat cgacatggag gcgctggtcg aggacctgcg tgacgacgcc gggatcctga
     5161 cgcacgacaa ggggctgcgg gtggacctgg tggagaacgc cccggcgacg atcctggggg
     5221 acgtcgcccg actccggcgc ctgttccgct cgctcatcgc caacgccgtg cgctacaccg
     5281 acgcgggcgg cttcatcggc atccgctcgc accgcgaggg aaacgcggtg cgcgtgtcga
     5341 tcgaggattc cggcatcggc atcccggagg agagcctgcc gcgcatcttc gatcgcttct
     5401 atcgcgccga tccggcgcgc gcgcgcgaca gcggcgggac cgggctgggg ctgtcgctgg
     5461 cccgctgggt ggccgaggct cacggcggga cgatcgcggt ggagagcgcc gtcggccgcg
     5521 gcagcacctt cacggtcacc ctgccgctgg cggggtgagc cggcaggccc gcggcctgcg
     5581 gtggtcgtgt cttccgagga ggaactgccc gaagggggca ggtgtattga ccctcgcgcg
     5641 acatggatgc tagggtccgg gcgattcggg gcggaaccgg acatttcttc ttcgtgaacg
     5701 tggcgcgaac cagcgccctt cggggaatcg acagaactgg tgcagtccag cgaggaccgg
     5761 gaggtgggga tgagacggca tctaagcgtg gcgttgcttg tgtgtctctt cgcggccggc
     5821 gcttcggtcg cgcacgccgg gaccggcgga aaaccggtga cctggcagtt cgatttcggc
     5881 tacagcatgg tgcaggggaa gccgagcgat gtcttcgagg acggctggac catcgggttc
     5941 ggcggagtcg cgcgcccctg gcccaagacg ccgctcggaa tccgctggga cttcacctac
     6001 gactggtggg acgtgaacac cggagaactg caaagcggac ctggcgtcat ccagatcgac
     6061 gacggcgacg gagaccagtg gtcactgcgc accggcatcc agtacgagtc tccgggggac
     6121 aagacgaagt tcatcggcgg cgtggggatc ggcggctacc gggtgagtgc cgacatctcg
     6181 aattcggtcg tggtcccggg ctggatctgc gatccttatt attggtggta ctgctacccc
     6241 ggtctggtcg agggggaagt cgtcatcgcc gacaagacgc tcaccaagtt cgggttctac
     6301 gccacggcgg ggatggtctt ccctctctcc agctcggaca tctacgtcga gcttcagtac
     6361 cactgggtga acgtcgagaa gtattacgag accatgccga tcgtcgtcgg ctggcgcttc
     6421 tagtttcggc ggaccgcacc gcccccgcga ccggggtggc cgcttcttcg ccgcgtccgg
     6481 cgggcttcag ggcttgccgg gcgcggcggc cttcgccgct tccgtcttct tcgcgagatc
     6541 cgcccggtag gtctgcaact gcccggggtt cagcaccccc gccatccgcc cctcggtctc
     6601 cgcctgcacc ttcttcacct cgtccccgag ccagcggcgc gttcctggtc cgccggcggc
     6661 gccggtgtgc gccgccagga tcgcccgctt cttctcgaag tcctccttga ggatcgggcg
     6721 aagcgcggtc tcctgctccg gcgtgagcgc cagcgggccc ttgagcgcgg cgatccgctg
     6781 atcgcagatc aagtcgcgga gcgagtcgtc gacctccttg cgcagcacgt ccaactccgc
     6841 cttctgcggc tcggtgagga tcggctgcag gctgtcccgg aaccgctcgc gcgtctgctt
     6901 gaactcctgc atgaacgacg gcagaacgcc ggcgcccccg ccggtgtatt cctcgaagag
     6961 cgcgcgaagt ttcttcacgt gggcgtccat cagcgggcgg accttctccg tctgctcggg
     7021 tgtgagcttg agccgctgct gcgcgagccg cagcgcctgg gccgacgctt cggagtcgga
     7081 gggggaggcg gcgctcgcgg gagcggccgg attcgcggcg gcgtccacgg tgtcggcgaa
     7141 ggcgggggag agcgtcaggc aggacagcga cagggcgaaa agcgcgcggg gcatcgggtt
     7201 ggacatggtc tcctcggtgc gtcggttgcc gccatcctac accacgcgcc ttgacacccg
     7261 gaatgcggct gtgccataga tgccgcatcc cggaaagcga aaggagcctc atgacgtcac
     7321 gaccgagccc gctgttcgga ctcaccctga tcgcggccct cttcgccgcg gcgccggcgt
     7381 cggcgcagac cgccaccgac gagaaggagc tcaccaagtc ggtgatcacg accgagcggc
     7441 aggcgatcgt cgccgccaac ctcaacctca ccgacgagca ggggacggtc ttctggcccc
     7501 tgtacaagca gtacggcgcc gagatggacg cgatcaccga tcgcaagatc cgcctgatcg
     7561 aggactacgc ccgcaagtac gtcggcatga ccgacgaaga ggcggccggg ctgatgagtg
     7621 cctacatggc catccagaag gacgagctga aggtgcgcga gcgctgggtc gtgaagatga
     7681 agaaggagct gccgatcaag acagtcctgc gcttcttcca gatcgagaac aagctggacg
     7741 ccatcgtccg cgcggagacc ctgcgcgtca ttccgctggt gcgcaaccag gcgatgccga
     7801 cttcgaaccg ctgagcgatg tctccgctgc cggccgcccc gcggcgctcc tggaggctgc
     7861 tgccgctgct ggcggtgctc gggctctccg tcccggcggc ggcgccgcgc ggcgc
//
LOCUS       DASRIQ010000492         9295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2271249, whole genome shotgun sequence.
ACCESSION   DASRIQ010000492 DASRIQ010000000
VERSION     DASRIQ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271249"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1378)
                     /locus_tag="VFD06_11970"
     CDS             complement(110..1378)
                     /locus_tag="VFD06_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZN04289.1"
                     /translation="MSPRRAETIAIGTELLGPDRIDSNGAFLSRVLGEHGYEVHFRTV
                     VGDDPKELEVALRIALGRADLVVATGGLGPTVDDLTREAASALLGLPLEEDPAILAEL
                     QARYRRHARAMPPTNRRQAQVPRGAEVLPNALGTAPGLLLRTGRAVLALLPGVPSEME
                     RMTLDHLLPRLDAAPERRVHRVLKIAGTNESDVDHRLADVHRSAGPVGWTILAGAGQI
                     EIHLSEWVPAGAAAAEVDRLDAAIASALGDDLFGRDQETLEDVVGRLLGERRQTLAAA
                     ESVTGGRIARRLTSVPGASNWFAGGAVVYTDTAKTSLADLPMEILSRHGAVSAETAVA
                     LAEGIRRRLAAAWGLATTGYAGPGGGTADMPPGTIFLGLAGPGGTTTRRLLLPGVRAV
                     VQARGAQAALDLLRRALLRQGVSDVQGGTP"
     gene            complement(1375..1980)
                     /locus_tag="VFD06_11975"
     CDS             complement(1375..1980)
                     /locus_tag="VFD06_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN04290.1"
                     /translation="MFYWFVKGIFYPFVRLYLGLTRDGTAHLPRRGPAIVVSNHVSYM
                     DAILLGSAAPRPIHFIVLRWMYDLLLLRWFYWGMGTIPVRAGRDAHGLRQAVRTLHTG
                     KVVGLFPEGSRSEDGRFGEAQPGAALLAALSGAPVVPVFIDGAGESMPVGGAFPRPAR
                     VHVRFGAPIRYPEGRGGRDREALAAFGRRLEEAIRALGPAA"
     gene            complement(1980..2681)
                     /locus_tag="VFD06_11980"
     CDS             complement(1980..2681)
                     /locus_tag="VFD06_11980"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZN04291.1"
                     /translation="MPTDRSKPRGLARLRQLFFTGLIIILPMMITVWLLAILVRIVEG
                     VSSPLLLAILRLVRPDVAVDPAIVGWVVPILGVFATIGLVLLVGALASNFIGRKVVEG
                     FERLMLRLPVVKGIYGSARQLIDAFSSTNASFRRVVAVEYPRPGIYTIGFLTRSDATI
                     SREGDQPMRDMSLVFLPTTPNPTSGWLAVVPNDQVVPLDMSIEEGVKLIVSGGLVAAG
                     PAACGSAGGERPTGS"
     gene            complement(2671..3255)
                     /gene="pgsA"
                     /locus_tag="VFD06_11985"
     CDS             complement(2671..3255)
                     /gene="pgsA"
                     /locus_tag="VFD06_11985"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZN04292.1"
                     /translation="MINLPTWITIGRIFLTPVLVVVLMTRTTALEHLNVSWQIIGVGV
                     FLLASLTDWLDGWLARRRNEVTTLGILLDPIADKLLTSAAFVSLVEIGAAKAWMVWII
                     LGREFAVDGLRMIASSQGVTISASVWGKYKTVSQVVAIVLLMFGNRYLGRWDLAGDVA
                     IWVVVALAILSATDYFMKFSRRLGVMGGEGARAD"
     gene            3374..4771
                     /locus_tag="VFD06_11990"
     CDS             3374..4771
                     /locus_tag="VFD06_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HZN04293.1"
                     /translation="MHPIRFGTDGWRGVIADDFTFEGVRRVTQAVADLIHEEGGTAAP
                     VPVGYDVRFLSRRFAECAAGVLEGNGFTALLPETATTTPMVSCQVVASAAPLGIAITA
                     SHNPPEWNGFKLKAAFGGSALPEMTERVEALLDRRAPRVGRAPLRRHPFLPSYVPRLM
                     AVVDVAAIRKAPLRVVVDSMHGAGARILEDLARGGRTRVRTVRAEPDILFGGAPPEPR
                     PEHLDLLRRTVLEQRAHLGLALDGDADRLGVIDERGRDVPPLQTFALLALHLLRVKGW
                     RGGIARTFANTLLAARIARAHKVPFHDLPIGFKHIARLMLEDDILIGGEESGGIGVKR
                     FLPERDGILIGLLLMEMLATGDRRLSRRIAAMQAEFGAYAYDRVDLRLPPRAGRRAVE
                     RLMQAPPDRLAGLKVIGRDTLDGLKLLFGDAGWILFRASGTEPVLRVYCEAATPVLVR
                     RLLRAGSARIRKLAA"
     gene            complement(4779..6788)
                     /locus_tag="VFD06_11995"
     CDS             complement(4779..6788)
                     /locus_tag="VFD06_11995"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HZN04294.1"
                     /translation="MSSTRRLAWAAGAVASAWLLLVGSLLQGRGGLEGTYSFVGAGGQ
                     EVEVSRRLDPRLDFPVPQRLDAAYIFHWDYQKFGFPSAMPPYVVRWRGLLSVPETGTY
                     GFSVDAQGAASLSIDGASIEIRPDATTDRPLSQGLHPIAVEYRLDSGDARFVLSWQPP
                     GRRQQPIPQDFLAPDRGHASASGGKTIGWGLLAAGVVAAVALWRRGGRSRDGFAGRLL
                     QGLQEERPRLALGAIVLLAAVLRFHDYALVPFHHETADEYQHAWEGWHLLHTLQPAAW
                     TTFPDAYPPDQAHDFRWFGDRYIVVRPYFDHPPLFSIPVGIVCSFAGARSFLECTLPA
                     MRLVPILLSLAGLLLLDRLARAYGCSERATLLAALVYAVVPVVILGQRLVKAESLLAL
                     LFMGALLAVRPNRPGGPARGAVTAGILGALSLWTKATGLGVVAVTAIALLAQRRRRDA
                     LVATGIAGAGLLLYLGYAAAYDFGIFLEVMQGQASSKWVSAESFRDLLGGKAVVKWFG
                     GGTYLWLWIALAVAALRRGRMTILVPVAVYATLLALSADHRVVYGWYRVPLYPFLCIA
                     AGVYLDEMLQESDLPRTFLFAMTAVVSALLYALPEAVGQSRPVLYLFAAAALLPFLPR
                     IVTERPWAHRLAAGGAIVLLLLFVVANVATVGGLLEIYSRTRGTQ"
     gene            complement(6785..7807)
                     /gene="add"
                     /locus_tag="VFD06_12000"
     CDS             complement(6785..7807)
                     /gene="add"
                     /locus_tag="VFD06_12000"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZN04295.1"
                     /translation="MAAPPSIGTELPKAEIHLHLEGSIDLDTLIAIRGRGKRMTSDGE
                     RKRLDLLYRHKGFIDFLGHFATLCEELTGPEDLALAATRLAERLAAQRVRYAEVMCSP
                     GIFVRRGIPTDEILDAVLEASAAAHARGGPRLQWILDSVRQWGPRAFEPLVEHAARFR
                     SRGVVGVGIGGDESGWTAQDFAMAYREARRLRLRTTAHAGEFSGPASVWEAIEVLGVD
                     RIGHGIRAAEDEKLLGLLAERRIPLECCPTSNVATGVVANWESHPIPRLHRAGAFVTV
                     GSDDPAMFGTSIQAEWDVLHRRLGLPPAEVLRIGRNTIEATFLEEPERRALLSEFDKA
                     AERHAS"
     gene            complement(7923..8570)
                     /locus_tag="VFD06_12005"
     CDS             complement(7923..8570)
                     /locus_tag="VFD06_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZN04296.1"
                     /translation="MRAVRVPVLAAALVASLVPALAPAAAGDALYYRRDADGNLVITN
                     VPDRRDLRVLTPERRQVRPQSGARYRDLIDAAAREHGLHPDLVYAVAAVESSFDPLAR
                     SVKGAQGLMQLMPETADRFGVRDAWNPDDNLRGGARYLRYLLDLFEGNVRLALAAYNA
                     GENIVLNLGRIPPYAETRTYVVKVMKLFGSQDPYLHEEAVLETPPETVAERPPAN"
     gene            complement(8567..>9295)
                     /locus_tag="VFD06_12010"
     CDS             complement(8567..>9295)
                     /locus_tag="VFD06_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZN04297.1"
                     /translation="LFGEKYGDQVRVVSVPGFSMELCGGTHAATTGSIGVVRLAPERG
                     IAAGTRRVEALTGAAALEEARDERRTLASVTAALHVDRQAAPEALSRLVEQNRALQRE
                     IEKLRVDIASGEGGGAADEVREVAGVRVLVPKVMRGLDKGALRALVDRNRERLPSGVI
                     VMWAAQEEDRVQVTTSVSRDLIPPLHAGEIVKALAPLVDGRGGGRADMAEAGGRRTGD
                     LEGVRERSIPILENLIRAATAGER"
BASE COUNT         1384 a         3432 c         3305 g         1174 t
ORIGIN      
        1 ccttccgcgc gcgtccagcg caccggcagg ggcagatcgc gcagcctcgc ctggcacgcg
       61 gcgaggcgcc cgcgcacaag ctccgggatc tcggccgcca cgaagagcct catggcgttc
      121 ctccctggac gtcgctcacc ccctggcgga gaagggcgcg gcgcaggagg tccagcgcgg
      181 cctgggcgcc gcgcgcctgc acgacggcgc gcacgccggg aaggagcagc cgcctcgtgg
      241 tcgtgcctcc ggggccggcg agcccgagga agatcgtccc ggggggcatg tcggcggtcc
      301 ctccgcccgg tccggcgtag ccggtggtcg cgagccccca ggccgccgcg aggcgcctgc
      361 ggatcccttc cgccagcgcc acggccgtct cggcggagac ggcgccgtgc cgggacagga
      421 tctccatcgg cagatcggcc agggacgttt ttgcggtgtc ggtgtagacg accgcaccgc
      481 cggcgaacca gttcgaggca ccggggacgg aggtgaggcg gcgcgcgatc cggccgccgg
      541 tcaccgactc cgcggccgcg agcgtctggc gccgctcgcc cagcagccgg ccgaccacgt
      601 cctcgagcgt ctcctggtcc cgcccgaaca gatcgtcgcc gagggccgag gcgatcgccg
      661 cgtcgagtcg atccacttcc gcggcggcgg cgccggccgg gacccactcg ctcaggtgga
      721 tctcgatctg ccccgccccg gcgaggatcg tccagccgac cggcccggcg ctccggtgca
      781 cgtccgccag ccggtgatcg acgtccgatt cgttggtgcc ggcgatcttc aggacgcggt
      841 gcacccggcg ctccggggcc gcatcgagcc tgggcagcag atggtccaat gtcattcgct
      901 ccatctcgct cggcacgccc gggagcagcg cgagcaccgc ccgcccggtg cgcaggagca
      961 gccccggagc ggtgccgagc gcgttcggga gcacctcggc gccgcgtggc acctgcgcct
     1021 gcctccggtt ggtcggcggc atcgcgcggg cgtgacggcg gtagcgcgcc tggagctctg
     1081 cgaggattgc gggatcctcc tcgagcggca ggccgagcag ggccgaggcc gcctcgcgcg
     1141 tcaggtcgtc caccgtcgga ccgagcccgc cggtcgccac gacgagatcg gctcgtccga
     1201 gggcgatccg gagcgccact tccagctctt tgggatcgtc ccccacgacg gtgcggaagt
     1261 gcacctcgta cccgtgctct cccaggacgc gcgacaggaa ggcgccgttg ctgtcgatcc
     1321 gatcggggcc gagcagctcg gtgccgatgg cgatcgtctc ggcgcggcgc gggctcatgc
     1381 ggccggcccc agggcgcgga tcgcctcttc gagacggcgg ccgaaggccg cgagcgcctc
     1441 ccggtcgcgc ccccctcgtc cctccgggta ccggatcggc gccccgaagc ggacgtgcac
     1501 ccgcgccgga cgcgggaagg cgccgccgac cggcatcgac tcccccgccc cgtcgatgaa
     1561 caccggcacg accggcgcgc cgctcaaggc ggcgaggagc gccgcccccg gctgcgcctc
     1621 cccgaaccgg ccgtcctcgc tgcgcgatcc ctcggggaag aggccgacca ccttgccggt
     1681 gtggagcgtc cgcaccgcct gtctcaggcc gtgcgcgtcg cgtcccgcgc gcaccgggat
     1741 cgtccccatc ccccagtaga accagcgcag cagcagcagg tcgtacatcc accggagcac
     1801 gatgaagtgg atcggccgcg gcgccgccga accgagcagg atggcgtcca tgtaggagac
     1861 gtggttggag acgacgatcg cggggccccg gcgcggcagg tgcgcggtgc cgtcgcgcgt
     1921 caggccgaga tagagccgga cgaacgggta gaagatcccc ttcacgaacc agtagaacat
     1981 tacgagcctg tcgggcgctc gccgccggcc gaaccgcagg cggccggacc cgccgcgacc
     2041 agcccgccgc tgacgatgag cttcaccccc tcctcgatcg acatgtcgag cggcacgacc
     2101 tggtcgttcg gcaccaccgc cagccagccg gaggtcgggt tgggggtcgt cggcaggaag
     2161 accagcgaca tgtcgcgcat cggctggtcc ccttcgcggg aaatggtggc atcgctcctc
     2221 gtcaggaacc cgatggtgta gatgccgggg cgcgggtatt cgaccgccac gacgcggcgg
     2281 aacgaggcgt tcgtgctgct gaaggcgtcg atcagctgcc gcgccgagcc gtagatcccc
     2341 ttcaccaccg gaaggcgcag catcagccgc tcgaaccctt ccaccacctt gcggccgatg
     2401 aagttgctgg cgagcgcccc gacgagaagc accagcccga tggtcgcgaa gacgccgaga
     2461 atcggcacga cccagccgac gatcgccggg tccacggcga cgtccggacg caccaggcgg
     2521 agaatcgcca gcagcagcgg cgacgaaact ccctcgacga tgcgtacgag gatcgccagg
     2581 agccagacgg tgatcatcat cggcaggatg atgatgaggc cggtgaagaa gagctggcgc
     2641 aggcgcgcga gtccgcgcgg cttcgaccgg tcagtcggca cgcgccccct cgcctcccat
     2701 cacgcccagc cggcgcgaga acttcatgaa gtagtcggtc gccgagagga tcgccagcgc
     2761 caccaccacc cagatggcga catccccggc gagatcccag cgtcccagat agcggttccc
     2821 gaacatcagc aggacgatcg ccacgacctg gctgaccgtc ttgtacttcc cccagacgct
     2881 ggcgctgatc gtcaccccct gcgacgaggc gatcatccgc agcccgtcca cggcgaactc
     2941 gcgcccgagg atgatccaga ccatccaggc cttggcggcg ccgatctcca ccagcgacac
     3001 gaaggcggcc gaggtcagga gcttgtcggc gatcggatcg agcaggatgc cgagggtggt
     3061 cacctcgttg cggcgccgcg ccagccagcc gtccagccag tcggtgagcg aggccagcag
     3121 gaacacgccg acgccgatga tctgccagga gacgttcagg tgctcgagcg cggtggtccg
     3181 cgtcatcagg accacgacca ggaccggggt caggaagatg cgaccgatgg tgatccaggt
     3241 cggaaggttg atcatctctc ggaggcagcg ggaggacgcg tcgattcgat caacggcgcc
     3301 gcggagcgcg gcggcgcgga acatagcaca cgcccggagg gggatcaacg ccgcctgtgg
     3361 tacaagcggc cgcgtgcatc ccatccgctt cgggaccgac ggctggcgcg gggtgatcgc
     3421 cgacgacttc accttcgagg gcgtgcggcg ggtcacgcag gcggtggcgg acctcatcca
     3481 cgaggagggg gggacggcgg cgccggtgcc ggtcggctac gacgtccgct tcctgagccg
     3541 ccgcttcgcc gagtgcgccg cgggggtcct cgaggggaac ggattcacgg cgctcctccc
     3601 ggagacggcg acgaccacgc cgatggtctc ctgccaggtg gtggcgtccg ccgcgccgct
     3661 cggcatcgcc atcaccgcca gccacaatcc gcccgagtgg aacggcttca agctcaaggc
     3721 cgccttcgga ggcagcgccc tcccggagat gaccgagcgg gtcgaagcgc tcctcgatcg
     3781 ccgcgccccg cgggtcgggc gggcgccgct gcgtcggcat cccttcctgc cgtcgtacgt
     3841 gcctcgtctc atggccgtcg tcgacgtcgc ggcgatccgg aaggcgccgc tgcgcgtcgt
     3901 cgtggacagc atgcacggcg cgggcgcccg catcctcgag gatctggcgc gcggcggccg
     3961 gacgcgggtg cggacggtgc gcgccgagcc ggacatcctc tttggcggcg cccctccgga
     4021 gcctcgcccg gagcacctcg atctcctgcg ccgcaccgtc ctcgagcagc gggcccacct
     4081 cggcctggcc ctcgacggcg acgccgatcg cctcggggtg atcgacgagc gggggcgcga
     4141 cgtgccgccg ctccagacct tcgcgcttct ggccctgcac ctgctgcgcg tcaagggctg
     4201 gcgcggcggc atcgcgcgca ccttcgccaa cacgctgctc gccgcgcgca tcgcgcgcgc
     4261 ccacaaggtg ccgttccacg atctgccgat cggcttcaag cacatcgccc gcctgatgct
     4321 ggaggatgac atcctcatcg gcggcgaaga gagcggcggg atcggcgtca agcgcttcct
     4381 gccggagcgg gacggcatcc tcatcgggct gctgctcatg gagatgctgg cgaccggcga
     4441 tcgccgcctg agccgccgca tcgccgccat gcaggcggag ttcggcgcct acgcctacga
     4501 ccgcgtggat ctgcgtctgc ccccccgtgc cggccgccgc gccgtcgagc gcctcatgca
     4561 ggcgccgccc gatcggctgg ccggcctcaa ggtgatcggc cgcgacacgc tcgacggcct
     4621 caagctcctg ttcggcgacg cggggtggat cctcttccgc gcctcgggga ccgagccggt
     4681 cctgcgcgtc tactgcgaag cggcgacgcc cgtgctcgtg cgccggctgc tgcgcgccgg
     4741 gtccgcccgg atccggaagc tggccgccta gaattcgctc actgcgtccc gcgggtgcgg
     4801 gagtagatct ccaggagccc gccgacggtg gcgacgttcg ccacgacgaa cagcaggagc
     4861 aggacgatcg ccccgccggc cgcgagccgg tgcgcccagg ggcgctcggt cacgatgcgc
     4921 ggcaggaacg gcagcagcgc cgcggcggcg aaaaggtaga gcaccggccg cgactgcccc
     4981 accgcctccg gcagggcgta caggagggcc gacaccaccg ccgtcatcgc gaacaggaac
     5041 gtgcgcggca ggtcgctctc ctgcagcatc tcgtcgaggt acacgcccgc cgcgatgcac
     5101 aggaacggat acagcggcac gcggtaccag ccgtacacca cgcggtgatc ggcggagagg
     5161 gcgaggaggg tcgcgtacac cgccaccggc acgagaatcg tcatccgtcc gcggcgcagc
     5221 gccgcgaccg ccagcgcgat ccacagccag agatacgtgc caccgccgaa ccacttcacc
     5281 accgccttgc cgccgagcag atcgcggaac gactcggcgg agacccactt cgacgaggcc
     5341 tgtccctgca tcacctcgag aaagatcccg aagtcgtagg cggccgcgta acccaggtac
     5401 agcaggagcc ccgccccggc gatcccggtc gcgacgaggg cgtcgcggcg ccgccgctgc
     5461 gcgagcaggg cgatcgccgt caccgcgacg actccgaggc cggtcgcctt ggtccagagc
     5521 gacagggcgc cgaggatccc cgcggtcacg gcaccccgcg ccggccctcc cggccggttc
     5581 gggcgcaccg ccagaagcgc ccccatgaac agcagggcga gcagactctc cgccttcacc
     5641 agccgctgtc cgaggatgac gaccggcacg accgcgtaca ccagcgcggc gaggagcgtc
     5701 gcccgctccg agcatccata cgcgcgcgcc aggcgatcga gcaggaggag gccggcgagg
     5761 ctcaggagga tcggcacgag ccgcatcgcc ggcagcgtgc actcgaggaa ggagcgggcc
     5821 ccggcgaagg agcagacgat gccgaccggg atcgagaaga gcggcgggtg gtcgaagtac
     5881 ggccgcacca cgatgtagcg gtcgccgaac cagcggaaat cgtgcgcctg gtcgggaggg
     5941 tacgcgtccg ggaaggtcgt ccaggcggcc ggctgcagtg tgtgcagcag gtgccaccct
     6001 tcccaggcgt gctggtactc gtcggcggtc tcgtggtgga acggcaccag cgcgtagtcg
     6061 tggaagcgca gcaccgcggc gaggagcacg atggccccga gcgccagccg cggccgctcc
     6121 tcctgaagcc cctgcaggag acgccctgcg aacccgtcgc gggagcgccc gccgcggcgc
     6181 cacagcgcca ccgcggcgac gacgcccgcc gcgagcagcc cccagccgat cgtcttccct
     6241 cccgacgccg acgcgtgccc ccggtccggc gcgagaaagt cctgcgggat cggctgctgg
     6301 cgccgccctg gcggctgcca ggacaggacg aagcgcgcgt ccccggagtc gaggcggtac
     6361 tcgaccgcga tcggatgcag accctgcgag agaggccggt cggtcgtggc gtcggggcgg
     6421 atctcgatcg acgcgccgtc gatgctgagg gacgcggccc cctgcgcgtc caccgagaag
     6481 ccgtacgtcc cggtttccgg gaccgagagc aggccgcgcc agcggacgac gtacggcggc
     6541 atcgccgacg gaaacccgaa tttctggtag tcccagtgga agatgtaggc ggcgtcgagg
     6601 cgctgcggca ccgggaagtc gagccgcgga tcgaggcgcc ggctcacctc gacctcctgc
     6661 ccccccgcgc cgacgaagga ataggtccct tcgaggccgc cgcggccctg caggagggat
     6721 ccgaccagga gcagccaggc cgaggcgacc gcgcccgccg cccaggcgag tctgcgcgtg
     6781 gagctcacga agcgtggcgc tccgcggcct tgtcgaattc ggacagcagc gccctgcgct
     6841 ccggctcctc gaggaacgtc gcctcgatgg tgttgcgccc gatgcggagg acctccgcgg
     6901 gcggcaggcc gagcctccgg tgcaggacgt cccactctgc ctggatcgac gtcccgaaca
     6961 tcgccgggtc gtcgctgccc acggtgacga aggcgccggc acgatgcagg cgcgggatcg
     7021 gatgcgactc ccagttcgcc acgacgccgg tcgcgacgtt actcgtcggg cagcactcga
     7081 gcgggatgcg gcgctccgcc agcaacccga ggagtttctc gtcctccgcc gcgcggatgc
     7141 cgtggccgat gcggtcgacg ccgaggacct cgatcgcctc ccacaccgac gccggcccgg
     7201 agaactcgcc cgcgtgcgcc gtggtgcgca gcctgagccg ccgcgcctcg cggtaggcca
     7261 tggcgaagtc ctgagccgtc cagcccgact cgtccccgcc gatcccgacg ccgaccacgc
     7321 cgcgcgaccg gaaccgggcg gcgtgctcca ccagcggctc gaaggcgcgc gggccccact
     7381 gccgcacgct gtcgaggatc cactgcaggc gcggcccgcc gcgcgcgtgc gcggccgccg
     7441 acgcttcgag gaccgcgtcg aggatctcgt cggtcgggat gccgcggcgc acgaagatcc
     7501 cgggggagca catcacctcg gcgtagcgga cccgctgcgc cgccagccgc tccgccagcc
     7561 gggtcgccgc gagcgccaga tcctcgggtc cggtcagctc ctcgcacagg gtggcgaagt
     7621 ggccgaggaa gtcgatgaac cccttgtggc gatagaggag gtcgagccgc ttccgctcgc
     7681 cgtcgctggt catccgcttc ccgcggccgc ggatggcgat cagggtgtcg aggtcgatcg
     7741 acccttccag gtggaggtgg atctccgcct tcggaagctc cgttccgatg gaagggggcg
     7801 cggccatgcg ggggattcta gcccggggcg aggagcctgt ccgagtcctg gcatgggccg
     7861 cgttcgccgt gccgcggcga acccgaaggg cgacccatgc cagaactcgg gcaggctcct
     7921 gctcagttcg cggggggacg ctcggcgacg gtttcggggg gcgtctcgag gaccgcttcc
     7981 tcgtgcagat acggatcctg gctgccgaag agcttcatga ccttcaccac gtatgtgcgg
     8041 gtctcggcgt agggggggat gcggccgagg ttcaggacga tgttctcgcc ggcgttgtag
     8101 gcggcgagcg ccaggcgcac gttcccctcg aacaggtcga gcaggtaccg caagtaacgg
     8161 gcccccccgc gcagattgtc gtccgggttc cacgcatccc gcaccccgaa gcgatcggcg
     8221 gtctccggca tcagctgcat cagcccctgc gcccccttca cggagcgggc cagcgggtcg
     8281 aacgacgact ccaccgccgc cacggcgtac acgaggtccg ggtgcagccc gtgctcgcgg
     8341 gcggccgcgt cgatgagatc gcggtaacgc gcccccgact gagggcggac ctggcggcgc
     8401 tccggggtga ggacccggag gtcgcgccga tccggaacgt tggtgatcac caggtttccg
     8461 tcggcgtcgc gccggtagta cagcgcgtct cccgccgcag cgggagcgag cgccggcacc
     8521 agggacgcca cgagcgccgc ggcgagcacg gggacccgga cggctctcac cgttcccccg
     8581 ctgtcgctgc gcggatgaga ttctcgagga tcgggatcga tcgctcccgg accccctcca
     8641 ggtccccggt gcgccgccca ccggcctcgg ccatgtcggc gcggccgccg ccgcgcccgt
     8701 ccacgagcgg cgcgagcgcc ttgacgatct cgccggcgtg cagcgggggg atcaggtcac
     8761 ggctgaccga ggtcgtcacc tggacgcgat cctcctcctg cgccgcccac atcacgatga
     8821 ccccggacgg cagacgctcg cggttgcgat cgacgagggc gcgcagcgcc cccttgtcga
     8881 gcccccgcat caccttggga acgaggaccc gcacgccggc tacctcgcgg acctcgtccg
     8941 cggcgccccc tccctcgccg gaggcgatgt caacccgaag cttctcgatc tcgcgctgca
     9001 gcgcccggtt ctgctccacg aggcgcgaca gcgcctccgg agccgcctga cgatcgacgt
     9061 gcagcgccgc ggtcaccgag gcgagcgtcc gtcgctcgtc gcgcgcctcc tcgagggcgg
     9121 cggcgccggt cagcgcctcg acccggcgcg tcccggcggc gatgccgcgc tcgggggcga
     9181 gccgcacgac gccgatcgac ccggtggtgg cggcgtgcgt cccgccgcac agctccatgc
     9241 tgaagccggg caccgagacg acgcggacct ggtcgccgta cttctcgccg aagag
//
LOCUS       DASRIQ010000493         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3064430, whole genome shotgun sequence.
ACCESSION   DASRIQ010000493 DASRIQ010000000
VERSION     DASRIQ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3064430"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFD06_12015"
     CDS             complement(<1..492)
                     /locus_tag="VFD06_12015"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZN04298.1"
                     /translation="MATPDHTLRGRLKALSIADLLVFLHGLGRSGRLHLTRDREEALL
                     DLRGPYVVRAAGNRDADRLEEALLRRGRITPEEYEAARRRQEEDPTAGIGRGLIAAGA
                     LTPRELIAARRDQAHQIVRGLFEWEDGEYRFEEGHAVSGWSAPVDLPILDLVAEGLRS
                     VRRL"
     gene            complement(717..>3198)
                     /locus_tag="VFD06_12020"
     CDS             complement(717..>3198)
                     /locus_tag="VFD06_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZN04299.1"
                     /translation="NDDGISKDTGSVSIVSGADGALLRKCVDPSASPLARLGSGLAAY
                     PDLDGDGKADVVAVAPFQHTAAGPRNGTALVLSSATCATLTRLDLNVGRSGTPLTNRG
                     VAVPGDLDGDHAAEIVIGTPWANGGDQGVGLALVFGRTGDCDGDGATPLGGDCDDAST
                     TRTPGLVEVCDGADNDCDLQVDEDSDGDGVAACADCDNGRASAYPGASETCNGLDDDC
                     DLLADDGPDADADGSTAVCDCSDQNPAIRPGAPEICNHRDDDCDAKVDETSPQVRDAA
                     ILTDPAGPANGRFGTGIAGIGDVDGDGVPDLAVGNGSNELLVVSGRSGAVRCRGFGRA
                     PFAGIKDISGDGVPDILAMGSTSVTILSGATCFSLQTCTLSGTSGFGASLVATGDINL
                     DGILDYAVGAPGSTATAPGGEVAVFNAGSCTPLYRVSDPTLSVSADFGRTVAAPGDLT
                     GDGWPDLVVGAPNDSSLGVTNAGRLVVVSGPDGTVVRRLHDPLAAPAHRLGTRIDSAG
                     DLNGDGIPDLFAYVAYDDVATDAGAVLQFSGTGDTVLRRCTDPLAADSDRFGSSLAAI
                     GDLTGDGVADFVAREMGNLPGASQAGVVLLVSSADCAIVARLSDPAPITAGTLALDGL
                     AVPGDLNGDGRPDIVAGADTGSPIPRGHVVVFSETSSCESPDNCPAVSNPFQLDSDGD
                     ILGNLCDTCPADPNLNQQDGDFDGAGDACDCQPANPAVRLPGDRLRIDVTSQSRILWT
                     AAVGAGGYYVWRGDLASRAYGAYGACWQHVIGPRAGDVGVVDATTPAVGSGFFYLVQG
                     GDSSCGRGLLGFDSLERPRVGSGPGVCP"
BASE COUNT          461 a         1137 c         1168 g          432 t
ORIGIN      
        1 gagccgccgc accgagcgca gcccctcggc gaccaggtcc aggatcggca ggtcgaccgg
       61 ggcggaccag ccggagacgg cgtgcccctc ctcgaagcgg tactcgccgt cctcccactc
      121 gaagagcccg cggacgatct gatgcgcctg gtcgcggcgg gccgcgatga gctcgcgcgg
      181 cgtcagggcg ccggcggcga tgagcccgcg gccgattccc gccgtggggt cctcctcctg
      241 gcggcggcgc gccgcttcgt attcctccgg ggtgatgcgg ccccggcgga gcagcgcctc
      301 ctccagccgg tcggcgtcgc ggttgccggc ggcccgcacg acgtagggcc cgcgcagatc
      361 gagcagcgcc tcctcgcgat cgcgcgtcag gtggagccgc ccggaccggc cgagcccgtg
      421 caggaagacg aggaggtcgg cgatgctgag cgccttgagc cgtcctcgca gtgtgtggtc
      481 gggggtggcc acggatgcgg cgcgattatc gtggacgcct cactccctgt caacgagggc
      541 cgggtgcgcg gcggcacgtc aagcgcgctg aatcgttcgg catggtgcgc gtcgtcaagt
      601 gggtgttgtc cgcgtgtgga cccacgggcg cagcccgcgc ggcgaccgcg tgccccgccg
      661 gcctccctcc cgttcccgcc ccgatcccga tgaaggagct tttgcgcacc cgaccgtcag
      721 ggacagacgc cggggccgct ccccacccgc ggacgctcga gcgagtcgaa gccgagcaat
      781 ccgcggccgc aggaactgtc gcccccctgc acgaggtaga agaagccgct gccgacagcc
      841 ggagtcgtcg catcgaccac gccgacgtct ccggcccgcg gcccgatgac gtgctgccag
      901 caggcgccgt aggcgccata ggcgcgcgat gccagatcgc cgcgccagac gtagtacccc
      961 ccggcgccca ccgcggccgt ccagaggatg cggctctgcg aagtcacgtc gatgcggagc
     1021 cgatcgcccg ggaggcgcac cgcgggattg gcgggttggc agtcgcaggc atcgccggcg
     1081 ccgtcgaagt cgccgtcctg ctggttcaga ttgggatccg ccggacaggt gtcgcagaga
     1141 ttcccgagga tgtcgccgtc ggagtcgagc tggaacggat tggagaccgc cgggcagttg
     1201 tccggagatt cgcagctgct cgtctcggag aagacgacga catggccgcg cgggatgggg
     1261 gaaccagtgt cggcgcctgc gacgatgtcc ggcctgccgt ctccgttcag gtcgccgggg
     1321 accgccagcc cgtccagcgc cagggtcccc gcggtgatcg gcgcggggtc gctgaggcgc
     1381 gcgacgatgg cgcagtccgc tgaagagacg aggagcacga cgccggcctg ggacgctcca
     1441 ggcaggttgc ccatctcccg ggcgacgaaa tccgccacgc cgtcaccggt cagatccccg
     1501 atcgccgcga gcgacgatcc gaacctgtcg gagtccgcgg ccaggggatc ggtgcatcgg
     1561 cgcaggaccg tgtccccggt gccggagaac tgcaggaccg cgcccgcatc ggtggcaaca
     1621 tcgtcgtacg ccacgtaggc gaaaaggtcc ggaatgccgt cgccgttgag atcccccgcg
     1681 ctgtcgatgc gcgtgccgag gcgatgcgcc ggcgccgcca gcggatcgtg caggcggcgg
     1741 acgaccgtcc cgtcggggcc cgagaccacg acgagccggc cggcattggt gacgccgagc
     1801 gacgagtcgt tgggggcgcc gacgacgagg tccggccagc cgtcgccggt gagatcaccc
     1861 ggtgccgcga ccgtccggcc gaaatctgcc gaaaccgaaa gcgtggggtc ggacacgcga
     1921 tagagggggg tgcagctccc ggcattgaag actgccactt ccccccccgg ggcggtcgcg
     1981 gtcgagccgg gggcccccac cgcgtaatcc aggatgccgt cgagattgat gtccccggtc
     2041 gcgacgaggg aagccccgaa accggaggtc cccgagagcg tgcaggtctg cagagagaag
     2101 caggtggccc cggacaggat cgtcacgctg gtggacccca tggcgaggat gtcgggcacg
     2161 ccgtctcccg agatgtcctt gatgccggcg aagggggcgc gaccgaagcc ccggcaacgc
     2221 accgcgccgg agcgcccgga cacgacgagg agctcgttcg aaccattgcc gaccgccaga
     2281 tccgggacgc cgtccccgtc cacgtccccg atccccgcga tccccgtgcc gaaccggcca
     2341 ttcgcgggac cggcggggtc ggtgaggatc gcggcatcgc ggacctgcgg cgaggtttcg
     2401 tcgactttgg cgtcgcaatc gtcgtcgcga tggttgcaga tctcaggagc acccggacgg
     2461 atggcgggat tctggtcgct gcagtcgcag acggcggtgg agccgtcggc gtcggcgtcc
     2521 gggccgtcgt ccgcgagcag gtcgcagtcg tcgtcgaggc cgttgcaggt ctcggaggcg
     2581 ccgggatagg ccgaggcgcg gccattgtcg cagtccgcgc aggccgcgac cccgtccccg
     2641 tcggagtcct cgtccacctg gaggtcgcag tcgttgtcgg ccccgtcgca cacctcgaca
     2701 agcccggggg tccgggtcgt actggcgtcg tcgcagtccc ctccgagcgg cgttgcgccg
     2761 tcgccgtcgc agtctccagt gcgtccgaac acgagcgcga gcccgacccc ctggtcccct
     2821 ccgttcgccc agggcgtccc gatgacgatc tcggcggcgt gatcgccatc gagatccccc
     2881 ggcaccgcca cgcccctgtt cgtgagaggc gtgcccgacc ggccgacgtt cagatccagc
     2941 cgggtcagcg tcgcgcaggt cgcgctcgac aggaccaggg cggttccgtt gcgcggcccc
     3001 gccgcggtgt gctggaacgg tgcgacggcg acgacgtcgg ccttgccgtc accgtcgaga
     3061 tccggatagg ccgccaggcc gctgccgagc cgcgcgagcg gcgaagcgga cgggtcgacg
     3121 cacttgcgca gcagggcgcc gtcggccccc gagacgatcg acacgctccc cgtatccttg
     3181 gagatgccat cgtcgttc
//
LOCUS       DASRIQ010000494         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_728560, whole genome shotgun sequence.
ACCESSION   DASRIQ010000494 DASRIQ010000000
VERSION     DASRIQ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728560"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFD06_12025"
     CDS             complement(<1..348)
                     /locus_tag="VFD06_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZN04300.1"
                     /translation="MAHEPKTRKTAASVAAYLNAIPDAGRRKDCKEIAAMMRRASKVA
                     PRMWGPGIIGFGSRRLTYASGRELDWPAIAFASRKGDLTLYLGEFDGKADLLKRLGKH
                     KTSKACLYIKRLAD"
     gene            393..1202
                     /locus_tag="VFD06_12030"
     CDS             393..1202
                     /locus_tag="VFD06_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZN04301.1"
                     /translation="MVGPGEAGRVFRRLHESGCFVIPNPWDLGSARALERLGFKALAT
                     TSSGFAWSQGRPDHGVSLHDTLEHLRAMAHGVKVPVNADLGFGFAVEPAALLVNVAAA
                     SLTGVSGLSIEDGTGDRDRPLFDFGLAVERIRAARRALDGTGVLLTARSEGFIVGWPD
                     LKETIRRLTAYAEAGADCLYAPGIRTREEITGVVSAVAPRPVNVLVGSSFASVHELAY
                     LGVRRISVGGALARAAWTGFLEAAREIAGGGTFHALDRALPYDAIDDGFEP"
     gene            1249..2634
                     /locus_tag="VFD06_12035"
     CDS             1249..2634
                     /locus_tag="VFD06_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HZN04302.1"
                     /translation="MTAGPDTSGWGGHPRGLSTLFFTEMWERFSYYGMRAFLILYMTT
                     EVAKGGLGFGVERAGSIYGTYTGSIWMATIGGGIVADRLLGAYRSVLWGGIIIALGHF
                     TLAFRALPFFYAGLTLIVMGTGLLKPNATTMLGTLYSREDARRDAGFSIFYMGINLGA
                     FIGPIIAGALAQRVDWHAGFACAGFGMLAGVIQYVLGRDRLRPVEENGPARPIGFAPP
                     APAVEPAAPGPGSVGAAARAGAPPASRFTRAEWMRMLAVCVFFVFAALFWGAYEQAAS
                     TLALFADQKVERVVFGWEIPSSWFVSIQALFVIILSPVAAWVWTKLGSREPSTPMKFS
                     YGLLAVGVSFVLLLPAGIMATGTAAPLVSALWLVAAYFIQVCGELCLSPVGNSVVTKL
                     APARVVGMMMGVWFLSIAAGNKLAGYAASLTATIALPQIFGAIAGITIAASIILMLLT
                     PSIRRMMGGVR"
     gene            complement(2631..3986)
                     /locus_tag="VFD06_12040"
     CDS             complement(2631..3986)
                     /locus_tag="VFD06_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN04303.1"
                     /translation="MSVPPAGEARPRGADGSTAPPSLIRAIGTVGLAAGIVNVTIGGG
                     IFRLPAVVAGVLGAQAPLAFLVCAAVMGLIVAAFAEAGKRVDRTGGPYAYVEVAFGPF
                     AGFLAGVLVWLLGTTAVAAVSTVLADSIGAIVPALSGAAGRAAVLVAVFAVLVTVNVI
                     GVGFGTRFNAVATAAKLLPLGLLIVAGAFAIEPAHLAWDEPMAPGSLARASMLLIFAF
                     CGVESALVPSGEIRDVARTVPRAVALAMVVVTAVYLAIQIVAQGILGAGLARETAPLA
                     AAAGSALGPWGGRLLLVGAAVSMFGYVSGMTLAVPRAVFAFARDGFLPASLGSVHPRF
                     FTPHLAIVAQSALVVALGITSGFEALAVVANLATLLLYAGCSLAAWRLAARDGAAAGR
                     PAAVIALCACCAIAWLLTSITWPEWRVAFLVLAAATLIYAAVRGSGRRPAPLGAPKTG
                     S"
BASE COUNT          578 a         1514 c         1481 g          586 t
ORIGIN      
        1 atcagcgagc cgcttgatgt agaggcacgc cttgctggtc ttgtgtttcc ccagccgctt
       61 caggagatcg gccttgccgt cgaactcgcc gagatacagc gtcaggtctc ccttgcgcga
      121 ggcgaaggca atcgccggcc agtcgagctc gcggccgctg gcgtatgtca ggcggcggct
      181 gccgaagccg atgatgcccg gtccccacat cctcggcgcg accttcgagg cgcggcgcat
      241 catcgcggcg atctccttgc agtccttgcg gcggccggca tcgggaatcg cgttgaggta
      301 cgcggcgacg ctggcggcag ttttcctcgt cttcggctcg tgggccatgc cggaagaata
      361 tagaatttcc gcttccagca ggagggacgt cgatggtggg accgggcgag gcgggccgcg
      421 tcttccgcag gctgcacgaa tcaggctgct tcgtcatccc gaacccgtgg gacctcggga
      481 gcgcccgggc gctcgagcgg ctcgggttca aggcgctcgc cacgacgtcg agcggcttcg
      541 cctggtcgca gggccgcccc gatcacggcg tgtccctgca cgacacgctc gaacacctgc
      601 gcgccatggc gcacggggtc aaggtgccgg tgaacgccga cctcggcttc ggcttcgccg
      661 tcgagccggc ggcgttgctc gtgaacgtcg ccgccgcgtc cctcaccgga gtgtccggcc
      721 tgtcgatcga ggacgggacc ggcgaccggg acaggccgct cttcgacttc ggcctcgcgg
      781 tcgagcgcat ccgtgcggcg cggcgcgccc tcgacggcac cggcgtgctc ctgaccgccc
      841 gctcggaggg gttcatcgtc ggctggcccg atctcaagga gacgatccgg cgactcaccg
      901 cctacgccga ggccggcgcc gactgcctgt acgctcccgg catccggacg cgcgaggaga
      961 tcaccggggt cgtgagcgcg gtcgcgccga ggccggtgaa cgtgctcgtc ggatcgagct
     1021 tcgcgtcggt gcacgagctg gcttatctcg gggtgcgccg catcagcgtc ggcggggcgc
     1081 tcgcccgcgc cgcctggacc ggcttcctcg aagcggcccg tgagatcgcc ggcggcggga
     1141 ccttccatgc gctcgaccgg gccctgccgt acgacgccat cgacgacggc ttcgagccct
     1201 aaggcgcggg cagcgccgca taacgatgtt cgggtagact ccgccgcgat gacggccgga
     1261 cccgacacct caggctgggg cgggcacccc cgcggcctct ccaccctctt cttcaccgag
     1321 atgtgggagc gcttctccta ctacgggatg cgcgccttcc tcatcctgta catgacgacc
     1381 gaggtggcga agggggggct cggcttcggc gtcgagcgcg ccggatcgat ctacggcacc
     1441 tacaccggca gcatctggat ggcgaccatc ggcggcggca tcgtcgccga ccggctgctc
     1501 ggcgcctacc gcagcgtgct gtggggcggg atcatcatcg cgctcggcca cttcaccctg
     1561 gcgttccggg cgctgccgtt cttctatgcc gggctgaccc tcatcgtcat gggcaccggg
     1621 cttctcaagc cgaacgccac gacgatgctc ggcaccctct actcccgcga ggacgcccgc
     1681 cgcgacgccg gcttctccat cttctacatg gggatcaacc tcggcgcctt cataggaccg
     1741 atcatcgcgg gggcgctggc gcagcgggtg gactggcacg ccggcttcgc ctgcgccggg
     1801 ttcgggatgc tggcgggggt catccagtac gtcctggggc gcgatcggct gcgtccggtg
     1861 gaggagaacg gccccgcccg gccgatcggt ttcgcgccgc ccgccccggc cgtcgagccg
     1921 gccgcgcccg gcccgggctc ggtcggcgcc gcggcgcgtg ccggcgcgcc ccccgcgagc
     1981 cgcttcaccc gcgccgaatg gatgcggatg ctggcggtct gcgtcttctt cgtcttcgcg
     2041 gccctcttct ggggggccta cgagcaggcc gcctcgacgc tcgccctgtt cgccgatcag
     2101 aaggtcgagc gggttgtctt cggctgggag attccgtcct cctggttcgt gtcgatccag
     2161 gcgttgttcg tcatcatcct gtcgcccgtc gcggcctggg tctggacgaa actgggatcg
     2221 cgcgagccgt ccacgccgat gaagttctcc tacgggctcc tcgccgtggg cgtctccttc
     2281 gtgctgctgc tgccggcagg gatcatggcg acgggcaccg ccgctccctt ggtgagcgcg
     2341 ctgtggctcg tcgccgccta cttcatccag gtgtgcgggg agctctgcct cagcccggtc
     2401 ggcaacagcg tggtcacgaa gctggcgccg gcgcgcgtcg tcgggatgat gatgggggtc
     2461 tggttcctgt cgatcgccgc cggcaacaag ctggccggct acgccgcgag cctcaccgcc
     2521 accatcgcgc tgccgcagat cttcggggcc atcgccggca tcaccatcgc tgcgtcgatc
     2581 atcctgatgc tcctgacgcc gtcgatccgc cggatgatgg ggggcgtccg ctaggagccc
     2641 gtcttagggg ccccgagcgg cgcaggccgg cgcccggatc cacggaccgc cgcgtagatc
     2701 agcgtcgccg cggccagcac caggaacgcc accctccact ccggccaggt gatgctggtc
     2761 aggagccacg cgatggcgca gcaggcgcac agcgcgatga ccgcggcggg ccggccggcc
     2821 gccgctccgt cgcgcgccgc cagccgccac gcggcgaggc tgcacccggc gtagagcagg
     2881 agcgtcgcga ggttcgcgac gacggcgagc gcttcgaacc cgctggtgat gccgagggcg
     2941 accacgagcg cgctctgcgc gacgatcgcc aggtgcggcg tgaagaagcg cgggtggacc
     3001 gacccgagtg acgccggcag gaagccgtcg cgggcgaagg cgaagacggc gcgcgggacc
     3061 gcgagggtca tgccgctgac gtaaccgaac atcgacaccg cggcgcccac caggagcagc
     3121 cgcccgcccc agggtccgag cgcgctcccc gccgccgcgg cgagcggcgc cgtctcgcgc
     3181 gccagccccg cccccaggat cccctgggcc acgatctgga tcgcgaggta gacggcggtg
     3241 acgacgacca tcgccagcgc gacggcgcgc ggcaccgtgc gtgcgacgtc gcgaatctcg
     3301 ccgctcggga cgagggcgct ctcgaccccg cagaacgcga agatgaggag catcgaggcg
     3361 cgcgccaggc tccccggagc catcggctcg tcccaggcga gatgggccgg ctcgatggcg
     3421 aacgccccgg cgacgatcag caggccgagc ggcaggagct tggcggcggt cgcgacggcg
     3481 ttgaaacggg tgccgaagcc gaccccgatc acgttgaccg tcacgaggac tgcgaagacc
     3541 gcgaccagca ccgccgcccg gccggcggcc cccgacaggg ccgggacgat ggcgccgatc
     3601 gaatcggcca ggacggtcga gacggccgca accgccgtgg ttccgaggag ccacacgagg
     3661 accccggcga ggaacccggc gaacgggccg aaggcgacct cgacgtaggc gtacggcccg
     3721 ccggtgcgat cgacgcgctt ccccgcctcg gcgaaggcgg cgacgatcag tcccatcacc
     3781 gccgcgcaga cgagaaacgc cagcggcgcc tgcgcgccca gaaccccggc gacgaccgcc
     3841 ggcaggcgga agatccctcc gccgatggtg acgttgacga tcccggccgc gagcccgacc
     3901 gtcccgatgg cgcggatcag ggacggcggc gcggtcgaac cgtccgctcc gcgcggtctc
     3961 gcctcgccgg ccggcggtac gctcacccgc ggcgcgcggc gccggcgcgc gtccggtgca
     4021 tccaggcggc ccataggtgg tacgagccgc cgaggacgac cgcgatcgcc gcggcgcccc
     4081 aggtcacccg tccggcacgc aggaagcgcc cgaggccgac gaggaacatc gggatgtaga
     4141 gcaccaagcc cgtgcggac
//
LOCUS       DASRIQ010000495         5464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_728762, whole genome shotgun sequence.
ACCESSION   DASRIQ010000495 DASRIQ010000000
VERSION     DASRIQ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728762"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5405)
                     /locus_tag="VFD06_12045"
     CDS             complement(<1..5405)
                     /locus_tag="VFD06_12045"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZN04304.1"
                     /translation="MGRAACGGGVLTTPLGAIEALEGQIGFAWGGTRVRLDPVILHGA
                     GIDLAGRGTLEGLGKGPTGPPALEGTFALGLDAAALARWLPSDASPSGRVDFKLGGAW
                     RQGAPRLEGTFSSAEARLFGVDARALQGSLRWDGALHLDDVKAAFFGGTVRGGVTVRP
                     RDSGGWQGETQVAAERLAAADLLKAAGWTGPGIDGAISYNGHHTFDQNGVPSLRGPGV
                     ATLDGSMRGPSGEARPLTARVEVEARGRTLAVIDGTLKAAATEARFSGSFSAAEGVRL
                     RLRGASGDLTDILPLFSASPHTHALLPSGVGGVPPGKAPLAITRAALIVALSAATAAP
                     PKRNAAGPGSGRRDAGDLAAIIKRLGGRWEWDGDLRAGAKGFDFDGKVAGRGLTWNGA
                     PLGDLEADLTFAREHLTIRRARLVAPNGGSVEGDGTVDFHGDGHLRLDGRFERLAGAL
                     IAAIGGFKLAPEGEISGEMNVRGSIAAPEIGARIADGHLALQGVEATSLHGDVSILPC
                     LLRSTGLEGMLGGAGVRLSGGFPYCPGPDPEGERLHLEATEVDLTTLTPLLGTVPLTG
                     RVSIEAEIEGGILDPRGTMTIAGTDLVLGGMRLGPADLEADIQGFVARLKGGLPERNL
                     TIAGQAEARSGVPIDVSLETAELRLRGADIDPTFPEDISFLLAGKVDVRGPLARPKSL
                     QAEARFARVRFEAGEAFAENEAPIAARLEAGRLRLEPAVLSGPGTRIAVSGEAAIDPK
                     EPVAVDLNGRFDLPLVRTFVQGLLAEGTGEVMLKVSGPREDPTFTGTLRIDAPHVRYP
                     ALPFPIDDVAARIAFDGFSAVIEDWKFRAGGGTVIGTGEMLLGKAGLSRGLASILAAD
                     IRLTGHGVSAEFPEGFRSVSDPDLHLVFDPTGLALEGTIDFVRAVYSRDFKIETLMLS
                     GRAPAPFDFDVPPAVVAGMRLDLRLRAPEELWMRNDFGRIEGEADLRVTGTAAQPSIT
                     GRVTALEGSTIDFNRVRYRVLSGTIDFNDPVIINPFFDLNAETTVADYQINLRVEGTV
                     DSFRYELTSDPPLSESDIVSVLLTGRPVGTLGAASSGLAPESVSAYLAGQLSQTLGNR
                     FLGRVAPDLIAIDPLEVVTTQGADPATRVTLGKQLTPDLRLTYSDLLGSGASASYQVD
                     YRVGRNVGLVSERTPEGTIAGDLRFTLPGKAPIAPWEATVAAREEVRIGAVKLEGTIG
                     LPEKEVLDHLRLKPGRRHERARVSDRADRLVAWYRRKGYLAAEVDIDELANAAGEIEL
                     TVHVNAGPRLDLRIEGTGGKGMLEEAVVPLFSQTLFFDETVEDARARIEALVRDRGHR
                     VAKVKASIDQTDPARTLIRFDVDEGPKTQAEVVRIEGARQIEEKRVRRVVRTHADSWL
                     RRGVVKGGRLAEDAAAIHTLYVERGFPEADVPPADVVPLGEDGRRAEVVFRINEGPRV
                     AVHGLRLEGVLGVGEERLHEVAGLEDGEPFIRGTIEAAVARVRRLYDDAGWPDARITW
                     RAERFAGQAGGDGGGADDEFEERDIIVSVVEGLRQQVSDVAIGANIITDDDTIRRALK
                     VEPYRMLSRADMLASQSRLYRLGLFRAVEVRPGPMPEIIGPPAPVASSETAPLGPETT
                     APGGPVTPPESAAPPVADSNVATPPEAPGTPGATEPALPAGTQEPAPAVPPESTEPYR
                     RPVQVLVREAPPLRQTFGFGYDTEEKIRGLYEIAHRNLFGSGRYLGLQLRASSLEQRG
                     AITYREQGVFGGRYDLLGSAYGLDEERPAFSGRTVGLLGQLSRDVTKATRLQWRYNLK
                     DVNLSEATTEFEGTTIRL"
BASE COUNT          736 a         2090 c         1900 g          738 t
ORIGIN      
        1 aggcggatcg tcgtcccctc gaactcggtc gtcgcctccg acaggttgac gtccttgagg
       61 ttgtagcgcc actgcagccg cgtcgccttg gtcacgtcgc ggctcagctg tccgaggaga
      121 ccgacggtgc ggccggagaa ggccggacgc tcctcgtcga ggccgtaggc cgatccgagc
      181 aggtcgtagc gcccgccgaa caccccctgc tcgcggtagg tgatcgcgcc gcgctgctcc
      241 aggctgctgg cccgcagctg caggccgaga tagcgaccgc tgccgaacag gttccggtgc
      301 gcgatctcat acaggccgcg gatcttctcc tcggtgtcgt agccgaagcc gaaggtctgg
      361 cgcaggggcg gcgcctcgcg caccagcacc tgcaccgggc ggcggtacgg ctccgtggat
      421 tccggaggga ccgcgggcgc gggttcctgc gtcccggccg gaagcgccgg ctcggtcgcc
      481 cccggcgttc ccggcgcctc gggcggagtc gcgacgttgc tgtcggcgac gggcggggcc
      541 gcggattccg gcggcgtcac cggccctccc ggggccgtcg tctccggacc gagcggcgcg
      601 gtctcggacg aggcgacggg ggcggggggg ccgatgatct ccggcatcgg ccccggccgc
      661 acctcgaccg cgcggaacag ccccagccgg tacagccgcg actggctcgc gagcatgtcg
      721 gcgcggctca gcatccggta cggctcgacc ttgagggccc gccggatggt gtcatcgtcg
      781 gtgatgatgt tcgcgccgat cgccacgtcg gagacctgct ggcgcagccc ctcgacgacg
      841 ctcacgatga tgtcccgctc ttcgaactcg tcgtcagccc cgccgccgtc cccgccggct
      901 tgcccggcga atctctcggc ccgccaggtg atgcgcgcgt ccggccagcc ggcgtcgtcg
      961 tacaaccgac gcacccgggc gaccgccgcc tcgatcgtgc cgcggatgaa cggctcgccg
     1021 tcctcgaggc cggccacctc gtgcaagcgc tcctcaccga ccccgaggac cccttcgagc
     1081 cgcaacccgt gcacggcgac gcggggaccc tcgttgatcc ggaacacgac ctcggcccgg
     1141 cggccgtcct cgccgagcgg gaccacgtcg gccggcggca cgtccgcctc cgggaagccg
     1201 cgctccacgt agagcgtgtg gatggcggcg gcgtcctccg ccaggcgccc ccccttgacg
     1261 acgccgcgcc gcagccacga gtcggcgtgg gtgcgcacga cgcgccgcac gcgcttctcc
     1321 tcgatctggc gcgccccctc gatgcgcacc acctccgcct gggtcttcgg cccttcgtcg
     1381 acgtcgaagc ggatgagcgt gcgcgcaggg tcggtctggt cgatcgacgc cttcaccttc
     1441 gccacgcggt ggccgcgatc gcgcaccagc gcctcgatgc gggcgcgggc gtcctccacg
     1501 gtctcgtcga agaacagggt ctgcgagaag agcggcacca ccgcctcctc gagcatcccc
     1561 ttccccccgg tcccctcgat gcgcaggtcg aggcggggcc cggcgttgac gtgcacggtc
     1621 agctcgatct cccccgcggc gttcgccagc tcgtcgatgt cgacctccgc cgccaggtac
     1681 cccttgcgcc ggtaccaggc gacgaggcga tccgcccggt cgctgacgcg cgcccgctcg
     1741 tggcgccggc cgggcttgag acgcaggtgg tcgagcacct ccttctccgg gagcccgatc
     1801 gtcccctcga gcttgaccgc gccgatgcgg acctcctcgc gcgccgccac ggtcgcctcc
     1861 cacggggcga tcggcgcctt gcccggaagc gtgaagcgca ggtcgccggc gatcgtccct
     1921 tccggggtgc gctcgctgac cagcccgacg ttgcgcccga cgcggtagtc cacctggtac
     1981 gaggcgctcg cacccgaccc gaggaggtcg ctgtaggtca gccggagatc cggggtcagc
     2041 tgcttgccga gcgtcacccg ggtcgccggg tccgccccct gcgtcgtgac cacctcgagc
     2101 gggtcgatgg cgatgagatc gggggcgacg cgaccgagga accggttgcc gagcgtttgc
     2161 gacagctgcc ccgcgaggta cgccgagacg ctctcgggcg ccagcccgct cgaggcggcg
     2221 ccgagcgtcc cgaccggacg gccggtgaga aggaccgaga cgatgtccga ctccgacagc
     2281 ggcgggtcgc tcgtcagctc gtagcggaac gaatcgaccg tcccctccac cctgagattg
     2341 atctggtagt cggcgacggt cgtctcggcg ttgagatcga agaacgggtt gatgatcacc
     2401 gggtcgttga agtcgatggt gccggacagg acgcggtagc gcacccggtt gaaatcgatg
     2461 gtgctcccct cgagcgcggt gacgcgcccg gtgatcgacg gctgtgccgc cgttccggtg
     2521 acgcgcagat ccgcctcccc ctcgatccgg ccgaagtcgt tgcgcatcca cagctcctcc
     2581 ggcgcccgca gcctcaggtc gagccgcatg cccgccacca ccgccggcgg cacatcgaag
     2641 tcgaacggcg ccggcgccct ccccgaaagc atcagggtct cgatcttgaa gtcacggctg
     2701 tacacggcgc gcacgaagtc gatcgtcccc tcgagggcga gccccgtcgg gtcgaagacg
     2761 aggtgcaggt cgggatcgga gaccgagcgg aatccctccg ggaactcggc gctcaccccg
     2821 tgcccggtca ggcggatgtc ggccgccagg atcgacgcga gcccgcgcga gagcccggcc
     2881 tttccgagga gcatctcgcc ggtcccgatc accgtgccgc cgccggcgcg gaacttccag
     2941 tcctcgatca cggcgctgaa gccgtcgaag gcgatccgcg ccgccacgtc gtcgatcggg
     3001 aacggcagcg ccgggtagcg cacgtgcggc gcgtcgatgc gcagcgtgcc ggtgaaggtc
     3061 ggatcctcgc gcggcccgct caccttgagc atgacctccc cggtcccttc cgccaggagc
     3121 ccctgcacga aggtgcgcac caacggcagg tcgaagcgac cgttgagatc gacggcgacc
     3181 ggctctttgg gatcgatcgc cgcctccccc gagacggcga tgcgggtccc ggggccggac
     3241 aggaccgccg gctcgagcct gaggcgcccc gcctcgaggc gggcggcgat cggcgcttcg
     3301 ttctcggcga acgcctcccc cgcctcgaac cggacgcgcg cgaagcgggc ctccgcctgg
     3361 agcgacttcg gccgggcgag cgggccccgc acgtccacct tccccgccag caggaaggag
     3421 atgtcctccg ggaaggtggg atcgatgtcg gcgccgcgca gccgcagctc ggcggtctcg
     3481 aggctcacgt cgatcggcac gccggagcgg gcctccgcct gccccgcgat cgtcaggttc
     3541 cgctcgggaa ggccgccctt gaggcgcgcc acgaacccct ggatgtcggc ctcgaggtcc
     3601 gccgggccga gccgcatccc gccgagcacg agatcggtcc ccgcgatcgt catcgtgccc
     3661 ctgggatcga ggatcccccc ctcgatctcc gcctcgatcg agacccggcc ggtgagcggc
     3721 acggtcccga gcagcggcgt cagggtggtc aggtcgacct ccgtcgcctc gaggtgcagg
     3781 cgctcccctt ccgggtcggg accggggcag taagggaacc cgccggagag gcgcacgccc
     3841 gcgccgccga gcatcccttc gagcccggtg ctgcgcagca ggcagggaag gatgctgacg
     3901 tcaccgtgca gcgaggtggc ctcgaccccc tggagcgcca ggtgtccgtc ggcgatgcgc
     3961 gcgccgatct ccggcgccgc gatcgacccg cgcacgttca tctcgccgga gatctccccc
     4021 tccggcgcga gcttgaaccc tccgatcgcg gcgatgaggg cccccgccag acgctcgaac
     4081 cggccgtcga gacggaggtg cccgtcgccg tggaaatcga ccgtcccgtc cccttcgacg
     4141 ctcccgccgt tcggcgcgac gagccgcgcg cgccgaatcg tgaggtgctc gcgggcgaag
     4201 gtcagatccg cctcgaggtc cccgagagga gcgccgttcc aggtgagccc gcgccccgcg
     4261 accttgccgt cgaaatcgaa ccccttcgcg ccggcgcgca ggtcgccgtc ccattcccat
     4321 cgtccgccga gccgcttgat gatcgcggcg agatcgccgg cgtcccgcct tcccgagccc
     4381 ggaccggcgg cgtttcgctt cggcggcgcc gcggtcgcgg ccgaaagcgc gacgatgagg
     4441 gcggcgcggg tgatcgccag cggtgctttc ccaggcggga cgcccccgac ccccgacgga
     4501 aggagggcgt gggtatgagg agaagcggag aacagcggca ggatgtcggt gagatcgccg
     4561 ctcgccccgc gcagccggag ccgcaccccc tcggcggccg agaacgatcc cgagaagcgg
     4621 gcctcggtcg ccgccgcctt cagcgtcccg tcgatcaccg cgagggtccg tccgcgcgcc
     4681 tccacctcga cccgcgccgt cagggggcgc gcctcgcccg aggggccgcg catcgacccg
     4741 tccagagtcg ccacccccgg accgcgcagg ctcgggacgc cgttctgatc gaacgtgtgg
     4801 tgtccgttgt aggagatcgc cccgtcgatc cccgggccgg tccatccggc cgccttcagg
     4861 agatcggcgg cggcgagccg ctccgccgcg acctgcgtct ccccctgcca gcccccggag
     4921 tcgcgcgggc gcaccgtgac gccgccgcgc accgttcctc cgaagaacgc ggccttcaca
     4981 tcgtccaggt gcagcgcgcc gtcccagcgc agggagccct gcagcgcgcg cgcatcgacc
     5041 ccgaagagac gcgcctcggc cgaggagaag gtgccttcga ggcgcggggc gccctggcgc
     5101 cacgctccgc cgagcttgaa atcgacgcgg ccggacggcg aggcgtcgga cggaagccag
     5161 cgggcgaggg cggccgcgtc cagtccgagc gcgaacgtcc cctcgagcgc cgggggaccg
     5221 gtcggacctt ttcccagtcc ctcgagcgtt ccgcgtcccg cgagatcgat cccggcgccg
     5281 tgcaggatca ccgggtccag ccggacccgc gtcccgcccc acgcgaaccc gatctgccct
     5341 tcgagcgcct cgatcgcacc gagcggcgtc gtcagcaccc cgccgccgca cgcggcgcgg
     5401 cccacgaact cggtctcaga cggtcccggc ccgcactcga ggctgaatcc atcgcacccg
     5461 aact
//
LOCUS       DASRIQ010000496         6796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_209623, whole genome shotgun sequence.
ACCESSION   DASRIQ010000496 DASRIQ010000000
VERSION     DASRIQ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209623"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFD06_12050"
     CDS             <1..622
                     /locus_tag="VFD06_12050"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HZN04305.1"
                     /translation="CDGVDFPAGSLSPSDKVREKDGWRLTWKFENLVTGQHIGIDLPN
                     RLNPGPVAARIIFFAPVSLLFFVTVLLVLGVLSGESLHPMHFFFMSAGFFSFHLLLAY
                     LVDHLDIHVSFLIASAVSIFLVVTYLVKVAGMTFAVQRAGVAQLVFLVLFSYAFFFEG
                     YSGLTVTIGSILTLFVLMQATARVNWNEVFAGPVAVRNREAGTGAS"
     gene            713..2914
                     /locus_tag="VFD06_12055"
     CDS             713..2914
                     /locus_tag="VFD06_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN04306.1"
                     /translation="MRARRAWMKSRLMQAIPFVLATAIALSGTISAKTDARLAYPETR
                     QVEQADDWHGTKVPDPYRWLESDPRVSQEVAQWIAAENKVTQGWLAQVPQREPIRKRL
                     TTLWNYERFSAPQWGGGHWFFARNTGLQNQSELFMTDSLTATPVQVLDPNSWSTDGTI
                     ALAGYAASDDGRYLAYARSVAGSDWTEWRVRDLQAGKDLDDLIQWTKFTFASWTTDSK
                     GFFYSRYDAPAKGEAFQAVNRNQKLFYHRLGTSQSQDVLVYARPDQPNWDFGGQVTED
                     GRYLVISVRKGTDPKNMLLYRDLNEPYGAPVELIGTFDNEYDFLGNDGTVFYFKTDLD
                     APKGRLIGIDVAQPETAKWRTIVPEAAEAMQGADMAGDTFLVSYLKDATSLVRQFKID
                     GTAVRDVALPGIGSAYGFGGKRTSKEVTYAYVSFNSPPTVYRYDLAGGNSSLWKQPKV
                     AFNPDDFVVKQVFYESKDKTKVPMFLVHRRDLKLDGNRPTLLYGYGGFKISMTPSFSP
                     ARLAWLEMGGVFAMPNLRGGGEYGEAWHKAGTKAQKQNVFDDFIAAAEWLVKNGYTKP
                     SRLAISGGSNGGLLVGACVTQRPDLFGATLPAVGVMDMLRFDQFTAGRYWVDDYGSAS
                     DPATFPALLAYSPYHNIKAGTKYPPTLVTTADTDDRVIPAHSFKFAAAMQKAQGGDAP
                     ILIRIETSAGHGGGKPTSKQIEEATDQYAFLVRTLGMEKEAKPPKAGRDGV"
     gene            complement(2970..5471)
                     /locus_tag="VFD06_12060"
     CDS             complement(2970..5471)
                     /locus_tag="VFD06_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HZN04307.1"
                     /translation="MTPDHPSLTPRSLPPLLASLALVLMAAASPAPERPADRRDGWTV
                     ERNDQCRDPHENDCAEAPSPFLLTRTDARVVVTGPVAHTVLRQDWTNPNRDPIEATYI
                     FPLPENAAVIALRLTIGSRTIEAEMRRREEARAIYEQARAEGRVAALLDQERPNVFAQ
                     RIANIMPGEKIEVTIDFDQEIRCDDGDCAYVLPTIVGPRFVPATTPDPGRIDPPVARP
                     GTDTGHRLSLTLDLDAGMPYHDLRTPGLRMDIEASRSGRARLRLGGEEGVRLDRDVQV
                     RYRLGGRDPEFGVLAWRDGEQPGTFTLYVQPPIQAEAESSAEPRELTFILDCSGSMSG
                     LPIEASKNVVRLALKAARPTDTLQILRFSDRASGLWSSPVPATSSNVRRALEYLDTLH
                     GEGGTQMLSGIRAALEPPPDPERLRIVALLTDGYIGNEVEILGEVRRLIGQGRLFAFG
                     IGSSVNRYLLEAIAQEGRGAVAFIGPRESPEAFVNRFVARIATPVFTDLRLTWEGIEV
                     DDLEPPRVPDLFAGQPLVIHGRYRDPGAGTLVLEGRLRGRREVLRRTVTLPARAADHE
                     ALGRLWARARIERLTRESYGQPDRAVVAAVTDLGLRFRLMTPWTSLVAVDQWVSNRGG
                     AARSIDVPVELPEGVDYEGIFGSNDAVGNRLRSLGYRGGGTSAKAFESSARPLSAAPP
                     PPPPPPAPMKSAEPGEEEAPLPGTEPDRRDGREQAAASSFVLVRVVEEDGTQRTVEAD
                     GEAWIARARRRSLVRTLTSEQFRALEEALRAGGAERWPEGDATYARGRARLVVVLADG
                     STRSVGLPSADPRIQAIIALVQAATFDTTGPADRF"
     gene            5655..6299
                     /locus_tag="VFD06_12065"
     CDS             5655..6299
                     /locus_tag="VFD06_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN04308.1"
                     /translation="MSGERASDAAPDPDLPDPEAALVLRAQQGDETAFAGLMDRHLDR
                     VWTLVWRVLRHREDTEDVVQEAFLAAWRSLPGFRREARFSTWLQTIAVSRALNHRARG
                     SERVRRASSPFATTGDPALEAARTVPAPREPADDRPASSPLRLLESAELRARLALCLE
                     KLPAEWRAVLALREGVESQSYGEIAATLGLALGTVRSRLARARLSLRDCVEGES"
     gene            6296..>6796
                     /locus_tag="VFD06_12070"
     CDS             6296..>6796
                     /locus_tag="VFD06_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN04309.1"
                     /translation="MRAGERPNHPIELMSASIDGELKPAEAAALEAHLEGCAECRTLL
                     ADFRRLDGPISEEVPPAVPADLRGRILARLASRTAAPPVPFWKQAMPLAAAASIVVAL
                     LLWYARPDRLPPLRATEPGPVPSMAAPAEGEQALASPEADRLLKEAPPPPPASSPVPA
                     PAPQKTS"
BASE COUNT         1020 a         2351 c         2412 g         1013 t
ORIGIN      
        1 ctgcgacggc gtcgatttcc cggccggcag cctgtctccc agcgacaagg tccgcgagaa
       61 ggacggatgg cggctcacct ggaagtttga gaacctcgtg accgggcagc acatcggcat
      121 cgatctcccg aatcgcctga acccggggcc ggtggccgcc cgcatcatct tcttcgcccc
      181 ggtctcgctc cttttcttcg tgacggtgct cctggtcctg ggagtcctga gcggggagag
      241 cctgcacccg atgcacttct tcttcatgtc ggccgggttc ttctccttcc acctgctcct
      301 ggcctacctc gtcgatcatc tcgacatcca cgtgtcgttc ctcatcgcct ccgccgtgag
      361 catcttcctc gtcgtgacct acctcgtgaa ggtggccggg atgaccttcg cggtgcagcg
      421 ggccggagtg gcgcagctgg tcttcctggt cctgttctcc tacgccttct tcttcgaggg
      481 gtactccggc ctgaccgtga cgatcgggtc gatcctgacc ctcttcgtcc tgatgcaggc
      541 gaccgccagg gtgaactgga acgaggtgtt cgccggcccg gtcgcggtcc ggaaccggga
      601 ggccggaacc ggggcctcct gacaacccgg gcggcccggc tcccgtataa tccctgcctt
      661 cgcactcagg gatcttcgaa accgcccctc cgcaggggtt gcaggagagc cgatgcgcgc
      721 acgccgtgcc tggatgaaga gccgtctgat gcaggccatc ccgttcgtcc tcgccacagc
      781 gatcgcgttg tccgggacga tctccgcgaa gaccgacgcc cgcctcgcct acccggagac
      841 gcgccaggtc gagcaggcgg acgactggca cggcaccaag gtgccggacc cgtaccgctg
      901 gctcgagtcg gatccgcgcg tgtcgcagga agtggcccag tggatcgcgg cggagaacaa
      961 ggtcacccag ggctggctgg cgcaggtgcc gcagcgggag ccgatccgca agcggctgac
     1021 gacgctgtgg aactacgagc gcttctcggc gccacagtgg ggcggcgggc actggttctt
     1081 cgccaggaac accgggctgc agaaccagtc ggagcttttc atgaccgatt ccctgacggc
     1141 caccccggtc caggtgctcg atccgaacag ctggtcgacc gacggcacca tcgccctcgc
     1201 cggctacgcc gcgagcgacg atggccgcta cctggcctac gcgcgttccg tcgccggctc
     1261 cgactggacc gagtggcggg tgcgcgacct gcaggcgggg aaagacctcg acgacctcat
     1321 ccagtggacc aagttcacct tcgcctcgtg gaccaccgac agcaagggat tcttctacag
     1381 ccgctacgac gcgccggcca agggggaggc gttccaggcg gtcaaccgga accagaagct
     1441 cttctaccac cggctcggga cctcgcagtc gcaggacgtg ctcgtctacg cgcgcccgga
     1501 tcagccgaac tgggacttcg gcgggcaggt gacggaggac gggcgctacc tcgtcatctc
     1561 ggtgcgcaag gggaccgatc cgaagaacat gctgctctac cgcgacctca acgagccgta
     1621 cggcgcgccg gtcgagctga tcgggacgtt cgacaacgag tacgacttcc tcggcaacga
     1681 cgggacggtc ttctacttca agaccgacct ggacgcgccg aaggggcgcc tgatcggcat
     1741 cgacgtcgcg cagccagaga ccgcgaagtg gcggaccatc gtccccgagg cggccgaggc
     1801 gatgcagggg gcggacatgg cgggggacac cttcctcgtc tcctacctga aggacgcgac
     1861 gtcgctcgtc cgtcagttca agatcgacgg caccgcggtg cgcgacgtgg ccctcccggg
     1921 gatcggcagc gcctacggct tcggcgggaa gcggacctcg aaagaggtga cctacgcgta
     1981 tgtctcgttc aacagtccgc cgaccgtcta ccgctacgac ctggcgggcg ggaacagcag
     2041 cctgtggaag cagccgaaag tggcgttcaa cccggacgac ttcgtcgtca agcaggtctt
     2101 ctacgagagc aaggacaaga cgaaggtgcc gatgttcctc gtgcaccgcc gcgatctcaa
     2161 gttggacggc aaccgcccga cgctgctcta cggctacggc ggcttcaaga tctcgatgac
     2221 cccctccttc tcgccggcgc gtttggcgtg gctcgagatg gggggcgtgt tcgcgatgcc
     2281 gaacctgcgc ggcggcgggg agtacggcga ggcgtggcac aaggccggga cgaaggcgca
     2341 gaagcagaac gtcttcgatg acttcatcgc cgccgccgag tggctggtga agaacggcta
     2401 caccaagcct tcgcgcctgg cgatctccgg cggcagcaac ggcgggctcc tcgtcggcgc
     2461 ctgcgtcacg cagcggcccg atctgttcgg cgcgacgctg ccggcggtcg gggtcatgga
     2521 catgctccgc ttcgaccagt tcaccgccgg ccgctactgg gtggacgact acggcagcgc
     2581 ctccgacccg gccacgttcc cggcgctcct cgcgtactcg ccgtaccaca acatcaaggc
     2641 gggcacgaag tatccgccca ctctcgtcac cacggccgac acggacgatc gggtcatccc
     2701 ggcccactcg ttcaagttcg ccgccgcgat gcagaaggcc caggggggcg atgcgccgat
     2761 cctgattcgc atcgaaacct cggccgggca cggcggtggc aagccgacga gcaagcaaat
     2821 cgaggaggcc accgatcagt acgccttcct cgtccgcacc ctcggcatgg agaaggaagc
     2881 caagccgccg aaggccggcc gggacggggt ctaaccggcg aggagcctgt ccggtcggct
     2941 gaccggacag gctccccccc gggctccggc tagaagcggt cggccggccc tgtcgtgtcg
     3001 aaggtcgcgg cctgcacgag cgcgatgatc gcctggatcc gcgggtccgc ggagggaagg
     3061 ccgacgctgc gggtcgatcc gtccgccagc accaccacca gacgggcgcg gccgcgggcg
     3121 taggtcgcgt ccccttccgg ccaccgctcg gcgccgccgg cgcgaagcgc ctcctcgagg
     3181 gcgcggaact gctccgacgt gagggtccgg acgaggctcc gcctgcgagc gcgggcaatc
     3241 caggcctcac cgtccgcctc gacggtgcgc tgcgtcccgt cctcctcgac gacgcgtacg
     3301 aggacgaacg acgaggcggc cgcctgttcc cggccatcgc gccgatccgg ctcggtgccc
     3361 gggagcgggg cctcctcctc cccgggctca gcggacttca tcggcgcggg gggaggcggc
     3421 ggaggcggcg gggctgcgga cagaggacgg gcgctcgact cgaacgcctt ggccgacgtg
     3481 ccgccacctc tgtatccgag ggaccgcagg cgattcccga cggcatcgtt cgagccgaag
     3541 atcccttcgt agtccacccc ttccggcaac tcgacaggca cgtcgatcga ccgcgccgcg
     3601 ccgccacggt tcgacaccca ctggtcgacg gcgacgaggg aggtccacgg ggtcatcagt
     3661 cggaagcgga gcccgaggtc ggtcaccgcg gcgaccaccg cccgatcggg ctggccgtac
     3721 gattctcgtg tcaggcgctc gatgcgcgcc cgggcccaca gccgcccgag cgcctcgtgg
     3781 tcggcggccc gggccgggag cgtgacggtc cggcgcagca cctcgcgccg gccgcgcagc
     3841 cggccttcga ggacgagcgt gccggctccc gggtcgcggt accggccgtg gatcacgagc
     3901 ggctgaccgg cgaagaggtc cggcacccgc ggcggctcca ggtcgtccac ctcgatccct
     3961 tcccacgtca gccgcagatc ggtgaagacc ggggtcgcga tgcgcgccac gaagcggttc
     4021 acgaacgcct cgggggactc gcgcggaccg atgaaggcga cggcgccgcg cccttcctgc
     4081 gcgatggcct cgagcagata acggttcacc gaggaaccga tgccgaaggc gaacaggcgc
     4141 ccctggccga tgagccggcg gacctcgccg aggatctcca cctcgttgcc gatgtacccg
     4201 tcggtcagca gggcgacgat ccgcagccgc tccgggtcgg ggggaggctc gagcgccgcg
     4261 cggatcccgg agagcatctg ggtcccccct tccccgtgca gcgtgtcgag gtactcgagg
     4321 gcgcgccgca cgttggagga ggtcgccggc accggcgagg accacagtcc cgaggcgcga
     4381 tcggagaaac gcaggatctg cagcgtgtcg gtcggccggg cggccttgag cgcgaggcgc
     4441 acgacgttct tcgaggcctc gatcggcagg ccgctcatcg acccggagca gtcgaggatg
     4501 aaggtcagct cgcgcggctc ggccgaagac tccgcctcgg cctggatcgg cggctggacg
     4561 tacagcgtga aggtgcccgg ctgctcgccg tcgcgccacg cgagcacgcc gaattccgga
     4621 tcgcggccgc cgaggcggta gcgcacctgc acgtcccggt cgaggcgaac cccttcctcc
     4681 ccaccgagcc gcagccgggc ccgccccgaa cgcgacgcct cgatgtccat ccggaggccg
     4741 ggggtccgga ggtcgtggta cggcatcccg gcatcgaggt cgagcgtcaa tgacaggcga
     4801 tggccggtgt cggtgccggg ccgggcgaca gggggatcga tgcgtccggg gtcgggcgtc
     4861 gtggcgggca cgaagcgcgg cccgacaatg gtcggcagga cgtaggcaca gtcgccgtcg
     4921 tcgcagcgga tttcctgatc gaaatcgatc gtcacctcga tcttttcccc cggcatgatg
     4981 ttggcgatcc gctgggcgaa gacattcgga cgctcctgat cgaggagagc tgcgacccgt
     5041 ccctcggcgc gggcctgttc gtagatggcc cgcgcctcct cgcgtcgccg catctccgcc
     5101 tcgatggtcc gggagccgat ggtgagcctc aaggcgatga ccgccgcgtt ctcggggaga
     5161 ggaaagatgt aggtcgcctc gatcggatcg cggttggggt tcgtccagtc ctggcgcagc
     5221 accgtatggg ccaccgggcc ggtgaccacc acccgggcgt cggtcctggt gaggaggaac
     5281 ggcgaggggg cttcggcgca atcgttctca tgggggtcgc ggcactggtc gttgcgctcg
     5341 accgtccagc cgtcgcggcg atcggcgggg cgctccggcg ccggcgaggc ggcggccatc
     5401 aggaccaggg cgagggaggc gagaagaggt ggaagggagc gcggggtgag ggaagggtgg
     5461 tcgggggtca tcgtcagttc ctcctgcgac ggttggaccg gcgaggctca cctgagttcc
     5521 ggcgatcatt ttcgtgatcg cgggaacttc cgcgggatgt tgcggtccaa gcgcgtgagg
     5581 accggccgga caaggcgtcc caggccgacg cgacccggat gagctttcgt gtcattcttg
     5641 aggccatgcc ccgcatgagc ggcgaacgcg ccagcgatgc cgcgcccgat ccggaccttc
     5701 cggatccgga agcggccctc gtcctgcgcg cccagcaggg ggacgagacg gcgttcgcgg
     5761 ggctcatgga tcggcacctc gaccgtgtgt ggacgctcgt ctggcgggtg ctgcgccacc
     5821 gcgaggacac cgaagacgtc gtgcaggaag cgtttctggc cgcctggcgc tccctccccg
     5881 ggttccggag ggaggcgcgc ttttcgacct ggctgcagac gatcgcggtg agccgggcgc
     5941 tcaaccaccg ggcgcgcggc tccgagcggg tgcggcgcgc ctcctcgccg ttcgcgacca
     6001 ccggcgaccc ggccctcgag gcggcgcgca ccgtccccgc tccgcgcgag ccggccgacg
     6061 atcgaccggc gtcgtcgccg ctccggctcc tggagtcggc ggagctgcga gcgcggctgg
     6121 cgctctgcct cgagaagctg ccggccgagt ggcgcgccgt gctcgccttg cgcgaggggg
     6181 tggaatcgca gtcgtacggg gagatcgccg cgacgctcgg gctggcgctc ggcacggtgc
     6241 ggtcgcgcct ggcgcgggcg aggctgtcgc tgcgggattg cgtggagggg gaatcgtgag
     6301 agcaggtgag aggccgaacc atcccatcga gctgatgtcg gcctcgatcg acggcgagct
     6361 caagccggcg gaagcggcgg cgctcgaagc ccatctcgag gggtgcgcgg agtgccggac
     6421 gctgctcgct gatttccgga ggctggacgg accgatctcc gaggaagtcc ctccggccgt
     6481 gcccgccgat ctccggggac ggatcctcgc gcgtcttgcg tcgcggacgg ccgcgcctcc
     6541 ggttccgttc tggaagcagg cgatgccgct cgcggcggcc gcgagcatcg tggtggcgtt
     6601 gctcctctgg tatgcccggc cggaccggct gccgcccttg cgtgcaacgg agccggggcc
     6661 cgttccgtcc atggccgcgc cggcggaggg ggagcaagcc ctggcttcac cggaggccga
     6721 tcgcttgctg aaggaggcgc cgccgcctcc gcccgcgtcc agccccgtac ccgctccggc
     6781 accgcagaag acttcg
//
LOCUS       DASRIQ010000497         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_439591, whole genome shotgun sequence.
ACCESSION   DASRIQ010000497 DASRIQ010000000
VERSION     DASRIQ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439591"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VFD06_12075"
     CDS             <1..1504
                     /locus_tag="VFD06_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04310.1"
                     /translation="ISEYASERVANREEIRKLLGGGGNPSVTGQPTGNAGTGQNPEVM
                     KRQFADEDSRRMGRLLNNLRTIAEGLAAWDGYKAIVFFGDGVPENPGQDYGLNDPRRA
                     LTQEISQLAFSAGSSGVVLHTVQTTGVAAGSASEVGASYRRSNVLSTLALDTGGIQRA
                     TNDLKGALDDIEIATHGYYLLSYLPEEPPDGRRHSISVKCKRNGVTLRHRQYFTRFTP
                     EEARTRSVQAAYQVPEIHDGLGAELSVIAGPVAGQERVVDVVVYVPAGRLLYLPQPGG
                     AAATVEVGLVVLDSGGRETLRLSRRVRLGVDTWRALHGGAISYDFYSRVRLPLAAQSL
                     TAVISDLHSGDLGAARVALDAAGQPPPAVHGLSLYAIGEKSLWVQVETARNASADVAP
                     AAFTIGPTLRSRFFAGEKVSCGFKGKASSAGSGAMRLEVRRGEQVVRSIEVPAGGAEA
                     GPSEPSSPSRGLDLPTQGLESGEYVLSVEEDRAGGPVQIGRLPFRIAARR"
     gene            1654..>2350
                     /locus_tag="VFD06_12080"
     CDS             1654..>2350
                     /locus_tag="VFD06_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HZN04311.1"
                     /translation="MGRTRREFLATCGAGAALWAGSRLIPREILVSPLEAEEIPPDRV
                     RELAAFALDRAKKAGATYADIRINRYRTQLVTLRSQPDFASGALNHVPSINETGTFGF
                     GVRVIAAGAWGFSGRHLVTKDAVAAAVKEAVAIARADAPLRREPVRLAKVGAFTDAYR
                     TPVKQDPFAVTVGDKLEFLRGLNSEIKKVDEIFSANGTIALRRESRFFASSEGSRIEQ
                     EVVHTSSELTATAV"
BASE COUNT          332 a          820 c          857 g          341 t
ORIGIN      
        1 catctccgaa tacgcttccg agcgggtggc gaaccgcgag gagatccgca agctcctggg
       61 gggaggggga aacccttcgg tcaccggtca gcccaccggc aacgcgggga cgggacagaa
      121 cccggaggtc atgaagcgcc aattcgcgga cgaggactcg cggcggatgg gtcggctcct
      181 caataacctg cgaaccatcg ccgaagggct cgccgcgtgg gacgggtaca aggcgatcgt
      241 attcttcggc gacggagtcc ccgagaaccc ggggcaggac tatgggctga acgatccgcg
      301 ccgcgccctg acccaggaga tcagccagct cgccttctcg gccgggtcgt cgggtgtcgt
      361 cctccatacc gtgcagacga ccggcgtcgc cgccggcagc gcgtcggagg tgggggccag
      421 ttaccggcgc tccaacgtcc tctccacgct ggccctcgat accggcggca tccagcgggc
      481 cacgaacgac ctcaaggggg cgctggacga catcgagatc gcgacccacg gctactacct
      541 gctgtcgtac ctgccggagg agccgccgga cggccgccgg cactcgatca gcgtcaagtg
      601 caagcggaac ggcgtgacgc tgcgccaccg ccagtacttc acccgcttca cgccggagga
      661 ggcccggaca cgcagcgtcc aggcggcgta ccaggtgccg gagatccatg acgggctcgg
      721 cgcggagctg tcggtcatcg ccggcccggt ggccgggcag gagcgggtcg tggacgtggt
      781 ggtttacgtc ccggccgggc ggctcctcta tctgccgcag ccggggggcg cggccgccac
      841 ggtcgaggtg gggctcgtcg tgctcgactc cggcggccgc gagaccctgc gcctgtcgcg
      901 ccgtgtgcgg ctcggcgtgg acacgtggcg ggcgctccac ggcggcgcga tctcgtatga
      961 cttctactcg cgcgtgcgac tgccgctcgc cgcgcagagc ctgacggcgg tgatctccga
     1021 cctccattcg ggcgatctgg gagcggcgcg cgtcgcgctc gacgccgccg gccagccgcc
     1081 cccggccgtg cacggcctgt ccctctacgc catcggggag aagagtctct gggtccaggt
     1141 ggagacggcg cggaacgctt cggccgatgt ggcgcccgcc gcgttcacca tcggccccac
     1201 cctgcgctcg cggttcttcg cgggggagaa ggtctcctgc ggcttcaagg ggaaggcgtc
     1261 ctccgccgga tccggcgcga tgcggctcga ggtccggcgc ggcgagcagg tggtgcggag
     1321 catcgaggtt ccggccggcg gtgcggaggc cggtccgtcg gagccgtcct cgccgtcgcg
     1381 cggactcgat ctgccgacgc aggggctcga gagcggcgaa tacgtcctct cggtcgagga
     1441 ggatcgggcc ggagggccgg tgcagatcgg ccggctgccg ttccggatcg cggcgcggcg
     1501 ttgaccctcc ggcgtggcgg atcgcgccga tcggtttgcg ccgccctctt cgccctctcc
     1561 gcgatcgccg ccgccccgcc ggaggaggcg ctcccttagg gcgcgtgtta cgctccgcgt
     1621 tcccgcaagg tcagggagtc gcacggaggg gagatgggcc ggactcgcag ggaattcctg
     1681 gcaacctgtg gcgccggggc cgcactctgg gcgggttcgc ggctcatccc cagggagatc
     1741 ctcgtcagtc ccctcgaggc ggaggagatc cctccggatc gcgtgcgcga gctggccgcg
     1801 ttcgctctcg accgggcgaa gaaggccggc gccacctacg ccgacatccg catcaaccgc
     1861 taccggacgc agctcgtgac gctgcgatcg cagcccgact tcgccagcgg cgcgctgaac
     1921 cacgtgccgt cgatcaacga gaccgggacg ttcggtttcg gcgtgcgggt gattgccgcg
     1981 ggggcctggg gattctccgg ccgccatctc gtgacgaaag acgccgttgc cgcggccgtg
     2041 aaagaggcgg tcgccatcgc gcgcgccgac gcgccgctgc ggcgcgagcc ggtgcggctg
     2101 gcgaaggtcg gcgctttcac cgacgcctac cgcacgccgg tgaagcagga tccgttcgcc
     2161 gtgacggtcg gggacaagct cgagttcctc cgcggcctca actccgagat caagaaggtg
     2221 gacgaaatct tctccgcgaa cggcacgatc gccctccggc gcgagtcgcg cttcttcgcc
     2281 tcgagcgagg ggagccgcat cgagcaggag gtggtgcaca cctcctccga gctgaccgcc
     2341 accgccgtcg
//
LOCUS       DASRIQ010000498         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1249746, whole genome shotgun sequence.
ACCESSION   DASRIQ010000498 DASRIQ010000000
VERSION     DASRIQ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249746"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFD06_12085"
     CDS             <1..440
                     /locus_tag="VFD06_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HZN04312.1"
                     /translation="AHHMTSPAPGGEGIGRAIDAALADARLTAAHVSFVNAHGTGTVL
                     NDRAEASALVRLFRRGADVPVNAVKPVTGHTLGAAGALEAILCVRAIGQGLLPPTIGC
                     DEPEPDFALDVVRGAARRQPVSTALSLSCGFGGHNVALLLGEA"
     gene            494..961
                     /locus_tag="VFD06_12090"
                     /pseudo
     CDS             494..961
                     /locus_tag="VFD06_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
     gene            complement(1235..>2326)
                     /locus_tag="VFD06_12095"
     CDS             complement(1235..>2326)
                     /locus_tag="VFD06_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN04313.1"
                     /translation="SLDAAPQPARDLVARYRNSYHCLLFKPVWLVETARGCPFRCNFC
                     SVWQLYGRSFRERSIGAVVEDLAACGDSVFIVDDLFWNHPDRSLALARALRERGVFKR
                     WILVQSRTDLVARRPDLLEAWRPLARDFDIFFGLEAPSDRGLDRLTKDATLAETIEAA
                     RVARALRYGVTGNFVVDPDWEEDDFRTLWDFVAAQKLERAGYTILTPLPGTDLYRDHP
                     SLRRDAPWFQYDMHHLLWEPRLGAPRFFELYAETWRRSILNLGGTKRWTDWMKQIRPA
                     QIPYLTRVLFRTQRLMRAEAYLTEYRNGMPESGHPGSARPGSVEDERGDVPGGIPRHH
                     ESGPVHPTGQRAADHGAGRSDRAGMFQDR"
BASE COUNT          291 a          830 c          894 g          311 t
ORIGIN      
        1 acgcccacca catgacctcc ccggcgccgg gaggagaggg gatcggccgc gccatcgacg
       61 cggcgctcgc cgacgcgcgg ctgacggcgg cccacgtgtc gttcgtcaac gcgcacggca
      121 ccggcaccgt gctcaacgat cgggccgaag cctcggcgct cgtgcgcctg ttccggcgtg
      181 gggcggatgt tcccgtgaac gcggtcaagc cggtgaccgg ccacacgctc ggcgccgcgg
      241 gggcgctcga agcgatcctg tgcgtccggg ccatcggcca gggcctcctc ccgccgacga
      301 tcggctgcga cgagccggag ccggacttcg ccctcgacgt cgttcgcggc gcggcgcgcc
      361 gccagccggt ttcgacggcg ctgtcgctgt cgtgcggctt cggcgggcac aacgtcgcgc
      421 tcctcctggg ggaggcgtga ggttcgtccg cttcgatccg gcgccggcgc tcgccggggt
      481 cggcatcgtc ggcgtgcgcg actccgggag cctcgcgctc gggctccagg ggccggtcgt
      541 cgacgccgag gcggcgctcg aatcgctctt cggaccggcc ggcccggagg cgcggcgccg
      601 cgatcgctac ggcgtgctgg cgctgctcgc cgcccgcgag gcgctggcgg aggcggggct
      661 gacggacgcg gcgcggcgga gcgaacgctg cggcgtgatg atcggcacga cgcacgcctc
      721 ctccgaacgc aacggtcgct atgcggagga tctcgccacg ggcgcgtccg cgctcagccc
      781 gtcgctgttc gtgcgcacga cctcgggggc ggcggccgcc gacgtggcat tcgccttcag
      841 gatggggggg cccgggcaga cgttcgtctc cggctggacc gcgggagccg aagcgctcgc
      901 ggcggcggcc cgcgccgtgg ccgccggcgc ggccgacctg atgctggccg gaggggtcga
      961 ggtccccggc ccgatcttcg tccacgccgg gccgttcacg atggaagcgg cggcgatcgc
     1021 cgtcgtggcg cgggacgccc cgccgcagcg gccgcgcctg ctcgcgtacg gtcgcggtcg
     1081 cggcaccgac ggggtcggcg cgatcctggc cgggctggcg cgggagtgcg gcatcgagtg
     1141 ccgcgttctc gtcgaggcga acgatccggg cagcgagtcc cgcctccggc gcgcggcgcc
     1201 gcccgcggcg gcggatccgg acgggcggtc ccggctatcg gtcctggaac atgccggcgc
     1261 ggtcggagcg gccggcgccg tgatcggctg cgcgctgtcc tgtgggatgg acgggcccgc
     1321 tctcgtggtg gcgcgggatc ccgccgggga cgtcgccgcg ctcgtcctcg accgatcccg
     1381 gccgggccga tcccgggtgc cctgactccg gcatcccgtt ccggtattcc gtgaggtacg
     1441 cctccgcccg catcagccgc tgcgtccgga agagcacccg cgtcaggtag ggaatctgcg
     1501 cggggcggat ctgcttcatc cagtcggtcc agcgcttcgt ccccccgagg ttcaggatcg
     1561 agcggcgcca ggtttccgcg tacagctcga agaaccgcgg cgcacccagg cgcggctccc
     1621 acaggagatg gtgcatgtcg tactggaacc agggtgcgtc acggcgcaac gaagggtggt
     1681 cgcggtagag gtcggtgccg gggagcggcg tgaggatggt gtagccggcc cgctcgagct
     1741 tctgcgccgc gacgaagtcc cagagggtcc ggaaatcgtc ctcctcccag tccggatcca
     1801 ccacgaagtt cccggtgacg ccgtagcgca gcgcgcgcgc cacccgggcc gcctcgatcg
     1861 tctcggcgag cgtcgcgtcc ttggtgagcc ggtcgaggcc gcgatcggag ggggcctcga
     1921 gtccgaagaa gatgtcgaag tcgcgcgcca gcggccgcca cgcctccagc agatccgggc
     1981 ggcgggccac gagatcggtc cggctctgca ccaggatcca gcgcttgaag accccgcgct
     2041 cccggagggc ccgcgccagc gccaggctcc gatcgggatg gttccagaac aggtcgtcga
     2101 cgatgaagac actgtcgccg caggcggcga ggtcctcgac gaccgcgccg atggagcgtt
     2161 ctcggaacga ccggccgtag agctgccaga cggagcagaa gttgcagcgg aaggggcacc
     2221 cgcgggcggt ctccacgagc cacaccggct tgaagaggag gcagtggtag gaattccggt
     2281 agcgcgccac caggtcgcgc gccggctgcg gcgccgcgtc gagcga
//
LOCUS       DASRIQ010000499         8079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1513975, whole genome shotgun sequence.
ACCESSION   DASRIQ010000499 DASRIQ010000000
VERSION     DASRIQ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513975"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFD06_12100"
     CDS             complement(<1..202)
                     /locus_tag="VFD06_12100"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZN04314.1"
                     /translation="MAASVRLGVNVDHIATLRQARKAADPDPVAAAMLAELAGADGIT
                     VHLRGDRRHIQDRDLETLRRTVR"
     gene            complement(285..1646)
                     /gene="glmM"
                     /locus_tag="VFD06_12105"
     CDS             complement(285..1646)
                     /gene="glmM"
                     /locus_tag="VFD06_12105"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZN04315.1"
                     /translation="MKRLFGTDGVRGVAGKFPLDEETVVRIGEALARTLTKEGRPPRV
                     VIGRDTRASGPDIEAALTRGIESQGGTVDRGGVLTTPAVACVTKALGYDAGVVISASH
                     NPYRDNGIKVFSGEGFKLPDALEMEIERQVLDGPANKNAAKQASGAGGGRHIDAGALQ
                     KTYLDALRGSVPAGTTFAGRRLVLDCAHGAASDLGPRLFRSLGAEVVALHDAPDGSNI
                     NEGCGALHPDRLGREVARQQAWLGLAFDGDADRCLPVDASGRVLDGDYVLCLAARDLR
                     RAGRLHGDTTVGTVMTNLWLERSLKEDGIRVVRAPVGDKYVLEEMQRGGFVLGGEQSG
                     HVIFLERATTGDGLLTGLMLMELLQRTGQDLAAWASTITPCPQILVNVPVRERPPLET
                     HPVIGPAIAAEEKLLKGRGRVLVRYSGTEPKARIMVEGESRSAIEDAVGRLTKVLEAT
                     IGA"
     gene            complement(1643..2371)
                     /locus_tag="VFD06_12110"
     CDS             complement(1643..2371)
                     /locus_tag="VFD06_12110"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HZN04316.1"
                     /translation="MRIFLQRNIRFKAFSFLIALVWWAVVHGEDARIKDFVVPIDYTN
                     LAQTLEQSGRVVDTVTVRLRAPEAVLRNVTEDRLTVTVDLGRAPLGEQHIPITGKMLR
                     VPGGTEVVRVAPAVIPVRVERRARREVPIVAEFSGSPPKGYARLGYAVTPPVATVEGP
                     ASEVAAVARALTGTILLEGEVSDMEFEVRPIPEAPPGSRVRILVPADPVLVQVRIGPA
                     PPAAVPGRATPPAGAAPTGRRDGR"
     gene            complement(2382..3257)
                     /gene="cdaA"
                     /locus_tag="VFD06_12115"
     CDS             complement(2382..3257)
                     /gene="cdaA"
                     /locus_tag="VFD06_12115"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HZN04317.1"
                     /translation="MIESLFPAAGPWTFGWRDALDILLMTALIYNVGLLLRGTRAFQV
                     LVGALVLLLAYWATGPDKPLRMVTFHQVLGNVLFYAPFAAIVLFQTAIRRALAALART
                     SVLRLSAHGMTAAMVDEILAAVATLAARRIGALLVIEREQSLPDQIESGIPLDATITQ
                     DLIVNIANPGSPLHDGAIILGEGRVRAASCFLPVTTDPRLSREYGSRHRAAIGLTEEY
                     DSVAIVVSEERGTVRAAVEGMLSEPLDAEGLRVFLRRHLELERIRRGESGTGPRKPSP
                     ETAGTRDGAPRPDAA"
     gene            complement(3254..4390)
                     /gene="folP"
                     /locus_tag="VFD06_12120"
     CDS             complement(3254..4390)
                     /gene="folP"
                     /locus_tag="VFD06_12120"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZN04318.1"
                     /translation="MAPAAGTVVLRIEGLEAAAVRALAQAARAAGGEAVPAPPRRHDQ
                     FLRAAGAPDPGRALVIRLPSPGREAFLASLRRRAPQAAAEIDSAMTAVERPAALPFLP
                     IGRRTFLMGVLNVTPDSFSDGGCFTDVKTAVRHALRMASDGADILDIGGESTRPGAPP
                     VGLRDELRRVVPVLEALQPALAHLPRGGPFVSIDTTKSEVARRAALLGATLVNDVSGM
                     TRDPAMPAVVAEHGVLVVLQHTRGRPRTMQRGVRYRAVVPEVAAHLRATMAAARRAGV
                     PPEHVLIDPGFGFGKRRPHNLALLRHLRALRSLGRPILVGASRKSFLRSDRDESPEAR
                     REAGLAAQALAVAGGADIIRVHDVREALRVARLCDAVLRGPSNR"
     gene            complement(4509..6443)
                     /gene="ftsH"
                     /locus_tag="VFD06_12125"
     CDS             complement(4509..6443)
                     /gene="ftsH"
                     /locus_tag="VFD06_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZN04319.1"
                     /translation="MNTLIKNVLVWTAIIIAVLVLYRFLVTGRTDQVELTFTKMMEDV
                     EAGRVSKVTITGNEIVGEFNQPTADGQAKRFLTYAPTYDDLVKDLRRYNVAIEARKPK
                     DPTYMVSLLTWLPFLILAAVWVFFFRQMQSGGNKALSFGKSKARLSSSQAKKVTFKDV
                     AGVDEAKEELAEIIEFLKEPQKFQKLGGKIPKGVLLMGPPGTGKTLLARAISGEANVP
                     FFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCFIFIDEIDAVGRHRGAGLGGGHDE
                     REQTLNQLLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRRVVVPRPDVKGRE
                     EILRVHTRKIPLAEDVSLEVLARATPGFSGADLANLVNEAALAAARVSKKVVFMDDFE
                     GSKDKVLMGSERRSLIISEQEKRLTAYHEAGHALVAYFMPHADPIHKVTIIPRGMALG
                     VTQQLPIDDRHNYTREYLESTLSVMMGGRVSEKMFMNVLTTGAGDDLNKATDMVRKMV
                     CDWGMSDKLGPLTFGRREQEIFLGRDIVQHKDYSEDTAQLIDEELKRFAREAYERAEH
                     VIGSNKEALERVALALLEYEVLDGEEVGTLVKGASVESLRAGRRPSTRPEAHPARPVV
                     ETARPDRAAALGTDPIKQPS"
     gene            complement(6557..7066)
                     /gene="hpt"
                     /locus_tag="VFD06_12130"
     CDS             complement(6557..7066)
                     /gene="hpt"
                     /locus_tag="VFD06_12130"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZN04320.1"
                     /translation="MNVSREVLKTEEQIAERVRALGRDITEFYRGEEISLLGILKGSF
                     IFMADLAREIRLPAQVGFLEIVTSRKSEFLTEVAFSSSFPIEGSHVLVVEDILDTGVT
                     LAYLSEQLSLHAPKSLKICTFLDKPHRRKVDLTPDFVGFEVPDRHVVGYGLDFQGKHR
                     NLPHLTWVE"
     gene            complement(7063..>8079)
                     /gene="tilS"
                     /locus_tag="VFD06_12135"
     CDS             complement(7063..>8079)
                     /gene="tilS"
                     /locus_tag="VFD06_12135"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZN04321.1"
                     /translation="RIALGHTLDDQAESLLLRLLRGSGRRGLAGIYPVVDGLYIRPLL
                     GLRRADLRAWLRARRLRWREDVTNRDLSLTRNRVRRRLLPLLERDFNPRATEVLARAA
                     NLLRDEEEWLESLTGEAYGAVASEQPQGVALDAERLGAMPRALRRRVARRAVARCLGH
                     LRGIDQRHVDRALALLQRGKPRSLDLPKGLRVSRDDDTLVFRVRDAERAETAEVAGMC
                     PVPGELRFPLLRLTARLAEEGKPLPKPGPDRALLDADLVKGPLVVRPRRPGDRFHPQG
                     APGTRRLKSFLIDRKVPVDERGRIPLVLSGEKIAWVVGHRIDDRFKITPATRRVLVLT
                     KETR"
BASE COUNT         1164 a         2846 c         2899 g         1170 t
ORIGIN      
        1 tccgcacggt gcgccgcagc gtctcgaggt cccgatcctg gatgtgacgc cgatcgccgc
       61 gcaggtgcac ggtgataccg tccgccccgg ccagctccgc cagcatcgcc gccgccaccg
      121 ggtccggatc cgccgccttg cgcgcctggc gcagggtggc gatgtgatcg acgttgactc
      181 ccagtcggac gctcgcggcc atggatctcc ttcgcttcgg agtcgttcgg ggcgccggcg
      241 gaggccggcg gaaaggagga ttctagcgcg gaccgcccgg gttctcaggc gccgatcgtc
      301 gcctcgagga ccttcgtgag ccggccgacc gcatcctcga tggcggagcg cgactccccc
      361 tcgaccatga tgcgggcctt cggctcggtg ccggagtagc gcaccagcac gcgcccgcgc
      421 cccttcagga gcttctcctc cgcggcgatg gccgggccga tgaccgggtg ggtctcgagc
      481 ggcgggcgct cgcgcaccgg cacattcacg aggatctgcg ggcatggggt gatggtcgag
      541 gcccaggccg cgaggtcctg gccggtgcgc tgcaggagct ccatgagcat caacccggtg
      601 aggagcccat cgccggtcgt ggcccgctcg aggaagatga cgtggccgga ctgctcgccg
      661 ccgaggacga agccgccccg ctgcatctcc tcgaggacgt acttgtcacc gaccggcgcg
      721 cgcaccaccc ggatgccgtc ctccttgagg gagcgctcga gccacaggtt ggtcatcacc
      781 gtaccgaccg tcgtgtcgcc gtgcaggcgc ccggcccggc gcaggtcgcg cgccgcgagg
      841 cacaggacgt agtcgccgtc gaggacccgt cccgaggcat ccaccggcag gcagcggtcg
      901 gcgtcgccgt cgaacgccag gccgagccag gcctgctgcc gagccacctc gcgcccgagg
      961 cgatccgggt gcagcgcccc gcatccttcg ttgatgttgc tgccgtccgg cgcgtcgtgg
     1021 agcgccacca cctcggcgcc caggctgcgg aagaggcgcg gcccgaggtc ggaggcggcg
     1081 ccgtgggcgc agtcgagaac cagccgccgg ccggcgaagg tggttcccgc cgggacggag
     1141 ccgcgcagcg cgtcgaggta ggtcttctgg agggcgccgg cgtcgatgtg ccgccctcct
     1201 cccgcgccgg acgcctgctt cgcggcgttc ttgtttgccg gcccgtcgag gacctggcgc
     1261 tcgatctcca tctccagcgc gtccggcagc ttgaaaccct cgcccgagaa gaccttgatg
     1321 ccgttgtcgc ggtaggggtt gtgggacgcg gagatgacga ccccggcgtc gtagccgagc
     1381 gccttcgtga cgcaggcgac cgcgggcgtg gtgaggacgc cgccccgatc caccgtcccc
     1441 ccctgcgact cgatgccccg cgtcagggcc gcctcgatgt ccggccccga ggcgcgcgtg
     1501 tcgcggccga tcacgacgcg cggcggccgc ccctctttcg tcagcgtgcg ggcgagcgcc
     1561 tcgccgatcc gcacgaccgt ttcctcgtcg agcgggaact tccccgcgac gccgcgcact
     1621 ccgtcggttc cgaagagccg cttcatcgtc cgtccctccg tccggtcggc gccgcgccgg
     1681 cgggcggggt cgcgcgcccc gggaccgcgg ccgggggggc ggggccgatc cgcacctgca
     1741 cgagcaccgg gtcggccggg acgaggatcc gaacccggct ccctgggggc gcctccggaa
     1801 tggggcgcac ctcgaactcc atgtccgaca cctccccctc gaggaggatc gtcccggtca
     1861 gggcccgcgc caccgccgcc acctcgctcg ccggcccttc gacggtcgcc accggcggcg
     1921 tcaccgcgta gccgaggcgc gcgtacccct tcggcggcga gccggagaac tccgcgacga
     1981 tcggcacctc tcggcgggcc cgccgctcga cccgcaccgg gatgaccgcc ggcgccacgc
     2041 gcaccacctc ggtgccgccc ggcacgcgca gcatcttgcc ggtgatcggg atgtgctgct
     2101 caccgagcgg agcgcggccg agatcgacgg tgacggtcag ccggtcctcg gtgacgttgc
     2161 gcagcaccgc ctccggggcg cggagccgca ccgtgaccgt gtcgaccacc cgccccgact
     2221 gctccagcgt ctgcgccagg ttggtgtagt cgatcggcac gacgaagtcc ttgatgcggg
     2281 catcctcgcc gtgcaccacc gcccaccaga cgagggcgat gaggaaggag aacgccttga
     2341 accggatgtt ccgctgcagg aagatgcgca tctccgtcgc ctcaggccgc gtcggggcgc
     2401 ggcgcgccgt cgcgcgtgcc ggccgtttcg ggggacggtt tccgcgggcc cgtcccgctc
     2461 tcgccgcggc ggatgcgctc gagctcgagg tggcggcgca ggaagacccg cagcccctcg
     2521 gcgtcgagcg gctcgctcag catcccttcg accgcggccc gcacggtgcc gcgctcctcg
     2581 gagacgacga tggcgacgct gtcgtactcc tcggtgaggc cgatggcggc acgatggcgc
     2641 gaaccgtatt cacgcgacag gcgcgggtcg gtggtcaccg gcaggaagca ggaggcggcc
     2701 cgcacccgcc cctcgccgag gatgatggcg ccgtcgtgca gcggcgagcc ggggttggcg
     2761 atgttgacga tgagatcctg ggtgatcgtg gcgtcgagcg ggatgccgga ctcgatctgg
     2821 tcgggaagcg actgctcccg ctcgatgacg aggagcgcgc cgatgcgccg cgccgccagg
     2881 gtggcgacgg cggcgaggat ctcgtccacc atcgccgcgg tcatgccgtg cgccgaaagc
     2941 cgcagcaccg aggtgcgcgc cagcgccgcc agggcccgcc ggatcgccgt ctggaacagg
     3001 acgatggccg cgaacggcgc gtagaacagg acgttgccga ggacctggtg gaacgtgacc
     3061 atccgcagcg gcttgtcggg gccagtggcc cagtacgcga ggagcagcac cagcgccccg
     3121 accagcacct ggaacgcccg ggtgccccgc agaagcagcc cgacgttgta gatgagggcc
     3181 gtcatcagca ggatgtcgag ggcatcccgc cagccgaacg tccagggacc cgcggccggg
     3241 aacaggcttt cgatcatcgg ttggacggcc cgcggaggac cgcatcgcag agccgggcca
     3301 cgcggagggc ctcgcgcacg tcgtggacac gaatgatatc agcgcctcct gcgaccgcca
     3361 acgcctgcgc ggcgagtccg gcctcgcggc gcgcctccgg cgattcgtcg cgatcggaac
     3421 ggaggaacga tttgcgcgac gcgccgacga ggatcggccg cccgaggctg cgcagcgccc
     3481 gcagatgacg gagaagcgcc aggttgtgcg gccggcgctt tccgaagccg aaccccggat
     3541 cgatgaggac gtgctcgggc gggacgccgg cgcgtcgtgc cgcggccatc gtcgctctca
     3601 agtgggccgc cacctccggc acgaccgcgc ggtaacgcac gccgcgctgc atggtgcgcg
     3661 gccggccgcg ggtgtgctgc aggacgacga gaacgccgtg ctccgccacg accgccggca
     3721 tcgccgggtc gcgggtcatg ccggagacgt cgttgacgag ggtcgcgccg agcagcgcgg
     3781 cccgccgcgc cacctccgac ttggtcgtgt cgatcgacac gaacggcccc ccgcggggca
     3841 ggtgggcgag ggcgggctgc agcgcctcca ggaccggcac gacccggcgc agctcgtcgc
     3901 gcagcccgac cggcggcgcg ccgggccggg tggactcccc gccgatgtcg aggatgtccg
     3961 caccgtccga agccatgcgc aaggcgtgcc tcacggccgt cttcacgtcg gtgaagcatc
     4021 ctccgtcgga aaaggagtcg ggagtgacgt tgagcacccc catgaggaag gtgcgccggc
     4081 cgatcggcag gaaggggagc gccgccggcc gctccaccgc ggtcatcgcc gagtcgatct
     4141 ccgccgccgc ctgcggtgcg cggcgccgga gcgaggccag aaaggcctcg cgtcccgggg
     4201 acggcaggcg gatgacgagc gcccggccgg ggtcgggcgc gccggcggcg cgcaggaact
     4261 ggtcgtggcg ccgcgggggg gccgggaccg cttcgcctcc ggccgcgcga gccgcctgcg
     4321 ccagggcgcg cacggcggcc gcctccagcc cctcgatcct cagaacgacg gttcccgcgg
     4381 cgggagccat gcgcggaagc ccgagatccg cgtcgtctcc ctgctcggga caggcgaaga
     4441 cgaggacgtt gtgccgatcc gccgaccggt cgaaaggggg cggagcggcc gcgctcaggg
     4501 gagagagcct aggaaggctg cttgatggga tcggtgccca gcgctgccgc gcggtcgggc
     4561 cgcgccgtct cgacgaccgg ccgggcgggg tgcgcctcgg ggcgggtcga cggccgccgg
     4621 ccggcgcgga gcgactcgac cgaggccccc ttcacgaggg tgcccacctc ttcgccgtcg
     4681 aggacctcgt actcgaggag cgccagggcg acccgctcga gcgcctcctt gtttgacccg
     4741 atgacgtgct cggcccgctc gtacgcctcg cgggcgaagc gcttcagctc ctcgtcgatc
     4801 agctgggcgg tgtcttcgct gtagtccttg tgctgcacga tgtcgcgccc gaggaagatc
     4861 tcctgctcgc gccgcccgaa ggtcagcggg ccgagcttgt cgctcatccc ccagtcgcag
     4921 accatcttgc gcaccatgtc ggtcgccttg ttgaggtcgt cgccggcgcc ggtggtcagc
     4981 acgttcatga acatcttctc ggagacgcgt ccgcccatca tcacggagag ggtgctctcg
     5041 agatactcgc gcgtgtagtt gtggcggtcg tcgatcggca gctgctgcgt gacgccgagc
     5101 gccatgccgc gcgggatgat ggtgaccttg tggatcgggt cggcgtgcgg catgaagtac
     5161 gccacgagcg cgtgcccggc ctcgtggtac gccgtcagcc gcttctcctg ctcggagatg
     5221 atgagggagc gccgctcgct ccccatcagg accttgtcct tcgatccctc gaagtcgtcc
     5281 atgaagacga ccttcttgct gacccgtgcc gcggcgaggg ccgcctcgtt cacaaggttc
     5341 gcgagatcgg cgccggagaa gccgggcgtg gcgcgcgcca gcacctccag ggacacgtcc
     5401 tcggcgagcg ggatcttgcg ggtatggacg cggaggatct cctcgcgccc cttgacgtcg
     5461 gggcgcggca cgaccacgcg ccggtcgaag cgcccggggc gcaggagcgc cgggtcgagc
     5521 acatcggggc ggttcgtggc ggcgatcagg atgacccctt cgttcgactc gaagccgtcc
     5581 atctcgacga ggagctggtt gagcgtctgc tcgcgctcgt cgtggccgcc gccgaggccg
     5641 gcgccacggt ggcggccgac ggcgtcaatc tcgtcgatga agatgaagca cggggcgttc
     5701 ttcttcccct gctcgaacag gtcccggacg cgcgaggcgc cgacgccgac gaacatctcg
     5761 acgaagtcgg acccggagat ggagaagaac ggcacgttcg cctcgccgga gatggcacgc
     5821 gccaggaggg tcttgccggt cccgggcggc cccatcagga ggaccccctt ggggatcttg
     5881 ccgcccagct tctggaactt ctgcggctcc ttcaagaact cgatgatctc cgccagctct
     5941 tccttcgcct cgtccacccc ggcgacgtcc ttgaacgtga ccttcttcgc ctggctcgag
     6001 gagaggcgcg ccttcgactt gccgaacgac agcgccttgt tgccgccgct ctgcatctgc
     6061 cggaagaaga agacccagac cgccgccagg atgaggaacg gcagccaggt gagcaggctc
     6121 accatgtagg tcgggtcctt cggcttgcgc gcctcgatgg cgacgttgta gcggcgaagg
     6181 tccttgacca ggtcgtcgta ggtcggcgcg tacgtcagga agcgcttcgc ctggccgtcc
     6241 gccgtcggct ggttgaactc gccgacgatc tcgttgcccg tgatggtgac cttcgagacc
     6301 cggccggcct cgacgtcctc catcatcttg gtgaaggtca gctcgacctg gtcggtccgt
     6361 ccggtgacga ggaagcgata gagcacgagg accgcgatga tgatggcggt ccagacgaga
     6421 acgttcttga tgagggtgtt cagcgggtca ctccccgggg atctctcccg gtatttttcg
     6481 gtaacagggg gtcaatatag aacgaaaacg tcaccctttc aacggcttga ggggagggat
     6541 tttccggcga cctgtcttat tcgacccagg tcaggtgcgg gaggttccgg tgcttcccct
     6601 gaaagtcgag cccgtacccc acgacgtgcc ggtcggggac ttcgaatccc acgaaatcgg
     6661 gggtcaaatc gaccttgcgc cggtgcggct tgtcgagaaa agtgcagatc ttcagcgatt
     6721 tcggggcgtg cagggacagc tgctcgctga gatacgccag ggtgaccccg gtgtcgagga
     6781 tgtcctcgac cacgaggacg tgcgacccct cgatgggaaa ggatgaggaa aaggcgacct
     6841 ccgtcaggaa ctcggacttg cgcgaggtga cgatctcgag gaagccgacc tgcgccggca
     6901 gccggatctc ccgggcgagg tcggccatga agatgaagga gcccttcagg atgccgagaa
     6961 gcgagatctc ctcgccgcgg tagaactcgg tgatgtcgcg gcccagggcg cggacgcgct
     7021 cggcgatctg ctcttcggtt ttcaggacct cgcgggacac gttcatcgag tctccttcgt
     7081 gaggacgagg acccggcggg tcgccggggt gatcttgaac cggtcgtcga tgcggtggcc
     7141 cacgacccag gcaatcttct caccggatag caccagcggg atccggccgc gctcgtcgac
     7201 cgggactttc cgatcgatga ggaacgactt gagccggcga gtcccggggg ccccctgagg
     7261 gtgaaagcgg tcccccggcc ggcgcggccg gacgacgagc ggccccttca ccaagtcggc
     7321 gtcgagcagg gcccggtcag gacctggctt cggcaggggt ttcccctcct ccgccaggcg
     7381 cgccgtgagc ctcaggagcg ggaacctgag ctctcccggg accgggcaca tccccgccac
     7441 ctccgcggtc tccgcgcgct ccgcgtcccg gaccctgaag acgagggtgt cgtcgtcgcg
     7501 gctgacccgc agacccttcg gaaggtcgag gctgcgcggc ttcccgcgct ggaggagggc
     7561 gagcgccctg tccacatgcc gctggtcgat gccgcgcaga tggccgagac accgggcgac
     7621 agcccggcgg gccacgcgcc gccggagggc ccggggcatg gcgccgagcc gttccgcatc
     7681 gagcgcgacg ccttggggct gttcggaggc gaccgctccg taggcctcgc cggtcagact
     7741 ctcgagccac tcctcctcgt cccgcaggag attcgccgcc cgggcgagaa cctccgtcgc
     7801 cctcgggttg aagtcccgct cgaggagcgg cagaaggcgg cgccgcacgc ggttgcgcgt
     7861 caggctcagg tcccggttcg tgacgtcctc ccgccagcgg agccgccgcg cccgcagcca
     7921 ggcgcgcaga tccgcgcgcc tcagaccgag cagcggccgg atatagaggc cgtccacgac
     7981 cggatagatg ccggcgagcc cgcgacggcc cgatcctcgc agcagacgca gcaggaggct
     8041 ctccgcctga tcgtcgagcg tatgcccgag ggcgatgcg
//
LOCUS       DASRIQ010000500         8694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3066596, whole genome shotgun sequence.
ACCESSION   DASRIQ010000500 DASRIQ010000000
VERSION     DASRIQ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066596"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3981)
                     /locus_tag="VFD06_12140"
     CDS             complement(<1..3981)
                     /locus_tag="VFD06_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04322.1"
                     /translation="MSRHAVRLQIRFALLSLVILAVLAPEAKALVPRPQEDLDRRVIP
                     APYVLPIPEAEEEGGAGPAAASAAAFRRRHPGPWTISFDRRTGRPSLVEGAGIPLFPG
                     RGNGLTAVSAGLAKAPARLEDIEPLGRAFLDAGGEFLRPREGELRLNTDRSAWLDDGA
                     LVYLDYDWLVDGLPVEGGRVFLRVRHGNIIQAGSARIGAGLPKATPAIDADEALALLF
                     EHALGRRDDDVVVEEPHLLYVARPVGAAVVPWAEGLRWHLVWRAAFQRALEPMTWSGD
                     IDARTGEVLAFEDRNRYARVTGGVHPRTVTDPEVVRPFTNVQVTTTAGQVATGEGGTF
                     AYPGGPAFTSLDGPFFRPSCVGCSNPLRAFAYVDRGTGDLSLGTGGIDVIGNGASTPA
                     ERNSFFHLNRVRSLASKWLAIPWLGTTIRINVNIPDVCNAFWNGSANFFRSGGGCNNT
                     GEIADVMYHEWGHGLDGNTNGGDSSTGEATGDIVSMSVVHDPHLGPGFDITGASVRIL
                     DSNLVGYQARVNNLDTFCLVCTGQCENGPFGHEVHCEGEIYGQTHWDMTQALVAKHGF
                     NTGWQTAERLFFLSLPQNDTMVPSSAQSAYSAYLAVDDDNGNLTDGTPNCLEIYNAFN
                     AHGIAGTACAGNTAGCARPPQPSLTATPGHAKIVLDWTASAGAANYRVLRADFSASQA
                     SLPMGAPQPGTHFEDTTVQPGVTYYYVIEAQTAGGCRSTIENAVAASALPDGRLALGT
                     PVVDDIPAGNRSGSADPGESIDLTLPLMNAPPAGAVSAASATLSTTTPNVTILNGAAS
                     YGAVPVGGASNGTAYRAALAPGLACGQTMNYTMTINPGDGGAATTAFVPVLVGESVPR
                     YFEDFEGDFQTWTAVPGSPAATAGQWAKGIPVVPNPTQAPYTWAWAPTGDASGSGQCM
                     LTGQNTSDSSGDVDGGETILLSPVIDLSGAAAARLRYVRWWASSSLTDTGDTLTVEVS
                     GNNGANWVVAETVGAGARNLGWQPVEIRLESLVALTSTFRMRVKARDTSTDTQVEAAI
                     DNVTIDEIVCDLTPPCFTAPTFAGLATAAPGASCAETDLAWSAGSTNCQNAQIEYNVY
                     RSLTPGFTPAPQNRVAAGIAGTAFHDALLQPGTNYHYIVRADDSRSGEDANTVARIVS
                     APISPDTVSPIFAGLVSASTGTGCGDTILQWQPALETCSTPVRYNVYRSTSPGFTPGP
                     ANLVASVLDTGYIDRALQPEQTYHYRVRATDTKENEEANGFERQAVANTLPLVVYQES
                     FEGSNGGWSPTAPNDAVSGNWEWGDPELTAVQPEDDATPPPGTKAWITGLAAGVGPGS
                     FDVDTGT"
     gene            4166..7777
                     /locus_tag="VFD06_12145"
     CDS             4166..7777
                     /locus_tag="VFD06_12145"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZN04323.1"
                     /translation="MTDRRASRCLLVCLLLSGMSGLVYQVAWVRSLELVFGATTFAIA
                     TVLASFMGGLAAGSAAAGALETRLRRFHPVRLYAACEVGIAVAALLVPLAFRLLVPLS
                     RVVWETFHASFAAFSLLRFVLCASVLLVPTALMGATLPILSRFVAALRPEESGGPGSR
                     VVADGAPGDRGSEPARRVGMLFAVNTAGAVVGCALAGLVLMPGLGLLGTQCAAVALNL
                     LAAGGAMWVGRGRPFDAAAVAAADRPAMAPEAGAVPGRAPVFIAAYAISGAVAMLYEV
                     AWSRSLVLVLGSSTYSYTTMLTTFLAGLTIGAWLGTRVLKAKADPLLAIALCQLMTAT
                     TVALGLFLAGELPFLYVQLDEALDPSARGLLFVQLALAALVMFPPTLGLGAMFPLTLG
                     GIGMTTAAAQRLVSRAYAWNTVGAIAGSLLGGFWLLPRLGTRDVLILGIAVNAAIAFA
                     GIALTRAGELRSTPRAALLVLIAVFGVHLALAAPEWRSDVMSSGVFRYADRYRGLDRA
                     GFRKRVRQSHGDMLFFEEGLTCTISVFRTTRSLTMLNNGKPDASVPPGLALVGAAGAG
                     PDVVVDAGGAGAAATAAVRSEPLGDLPTQVLVGQIPLLLADRIDDVMVVGLGSGVTLG
                     SVLTHDVKKVDTLELEAAVVHGSRLFDHHSGAPLEDPRVSLVVNDARNDLLVRDRRYD
                     VIISEPSNPWIPGAASLFTRDFFRLARRRLQPHGVLCQWVQLYELWPEDFQAILRSFA
                     EVFPSIQIWRVGSDAILVGGPRDIPLPVGRVFARASDRVRGDLARIGLRGPEDLLAHF
                     WIGGDELRAAIPPGPINTDDNMRIEFAAPLRMLARDNERLERQKQELRGMFAGRTSAA
                     LPHLRFPADDPTREGAFLARLARAALERGYEDEAAVYAAAAWQRARGPETASTRAVVL
                     AVLGRSEDAAAARDEAERGWPDDPGVQRMLLDAAVVNEEPEAIRRHAQAVLRAAPDDA
                     SARYELAKVLDAVGENDAALRAILPLRALFAPAVARASGAPGPAATAPDGAARVLGLL
                     LAAAGRAAEAEPALRAHLAAHPDDAAVLTALAGAMRAAGRDAEAAAIERRLDPDAKSQ
                     AATRLAAASRAFESGRYDAARTALEEALQFAPDEEAAALLLARTLQRLGDTPGAADLL
                     KTWLASHSDRPLALGLLSQVLLDLGQPDGAAAAATRYRALTGEDWAGMDVIASLPSR"
     gene            7818..8336
                     /locus_tag="VFD06_12150"
     CDS             7818..8336
                     /locus_tag="VFD06_12150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN04324.1"
                     /translation="MSFKRLSTERVYAGRLLKIDRDRVLLPNGGTTELEMVRHPGAAA
                     VVPFITDQEILLVRQFRYAAQGFIYEVPAGTLHQGEAPEACAAREVQEEVGHRAGTLQ
                     HLASIYTTPGFTDEVIHLYTARDLEPAAQKLDHDEVLTVERLPFAEALRMIRDGRIVD
                     AKSICALMLARP"
     gene            8377..>8694
                     /locus_tag="VFD06_12155"
     CDS             8377..>8694
                     /locus_tag="VFD06_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04325.1"
                     /translation="MSALETLEALPMDVLKSRLDPKGSEFQENARHHQRLAEELRTRH
                     ARVKEGGPRHAAATQKKRGKLPVRERVARLLDDNTPFLELSALAAWGLHEGDAPGAGL
                     VTGI"
BASE COUNT         1129 a         3035 c         3222 g         1308 t
ORIGIN      
        1 cgtgccggtg tccacgtcga aggaaccggg tccgacgccg gccgcaagcc cggtgatcca
       61 ggccttggtg ccgggcggag gcgtggcgtc gtcctccggc tgcacggcgg tcaattccgg
      121 gtctccccat tcccagttgc cggagaccgc gtcgttcggc gccgtcggcg accagccgcc
      181 gttgctcccc tcgaacgact cctggtagac gacgagcggc agggtgttcg ccacggcctg
      241 gcgctcgaag ccgttggcct cctcgttctc cttcgtatcg gtggcccgga ccctgtagtg
      301 gtaggtctgc tccggctgca gcgcgcgatc gatgtagccc gtgtcgagga ccgaggccac
      361 gaggttggcc ggaccggggg tgaaaccggg ggaggtcgag cggtagacgt tgtagcgcac
      421 cggggtcgag caggtctcca gcgccggctg ccactgcagg atcgtgtcgc cgcagccggt
      481 cccggtcgaa gccgacacga ggcccgcgaa gatcggcgac accgtgtcgg gcgagatcgg
      541 tgcgctcacg atgcgcgcga cggtgttggc gtcctccccc gagcgcgagt cgtcggcgcg
      601 cacgatgtag tggtagttcg tcccgggctg cagcagcgcg tcgtggaagg cggtgccggc
      661 gatgccggcg gcgacgcggt tctgcggcgc cggcgtgaac ccgggtgtga gcgagcggta
      721 gacgttgtac tcgatctggg cgttctggca gttggtgctg ccggcgctcc aggcgaggtc
      781 ggtctcggcg cacgaggccc ccggcgcggc ggtcgcgagg ccggcaaagg tcggcgcggt
      841 gaagcacggc ggtgtcagat cgcagacgat ctcgtcgatc gtcacgttgt cgatcgccgc
      901 ctccacctgg gtgtcggtgg acgtgtcgcg cgccttgacg cgcatgcgga acgtcgaggt
      961 gagcgcgacg agggactcga ggcggatttc gaccggctgc cagccgaggt tgcgggcgcc
     1021 tgcgccgacg gtctcggcga cgacccagtt cgctccgttg ttgccgctca cctcgaccgt
     1081 gagcgtgtca cccgtgtcgg tgagggagga actcgcccac cagcgaacgt agcggaggcg
     1141 cgcggccgcg gcacccgaga gatcgatgac cggagacagc aggatcgttt cgccgccgtc
     1201 gacgtcgccg ctcgaatcgg aggtgttctg gccggtcagc atgcactgcc cgctgccgct
     1261 ggcgtcgccg gtcggggccc aggcccaggt gtacggggct tgtgtcgggt tcgggacgac
     1321 cggaatcccc ttcgcccact ggccggcggt cgccgccggc gatccgggca cggcggtcca
     1381 ggtctggaaa tccccctcga agtcctcgaa gtagcgcggc acgctctccc cgacgaggac
     1441 cgggacgaac gcggtcgttg cggcccctcc gtcacccgga ttgatcgtca tggtgtagtt
     1501 catggtctgg ccgcaggcga gcccgggggc gagggcggct cgataggcgg tcccgttcga
     1561 cgcgcctccg accggtacgg cgccgtacga ggcggcgccg ttgaggatcg tgacgttcgg
     1621 cgtcgtggtg gagagggtgg ccgaggcggc cgacaccgcg cccgccggcg gcgcgttcat
     1681 gagcggcagg gtgaggtcga tcgactctcc cgggtcggcg gacccactgc ggttgccggc
     1741 cgggatgtcg tccaccacgg gggtgcccag cgccagccgc ccgtcgggaa gcgccgaggc
     1801 ggcgaccgcg ttctcgatcg tcgagcggca gccgccggcg gtctgcgcct cgatgacgta
     1861 gtagtaggtc acgccgggct ggaccgtcgt gtcctcgaag tgcgttccgg gctgcggcgc
     1921 tcccatcggc agggaggcct gcgatgcgga gaagtcggcc cgaagaacgc ggtagttggc
     1981 ggccccggcc gaagcggtcc agtcgaggac gatcttcgcg tgtccgggtg tcgccgtgag
     2041 cgacggctgc ggtgggcggg cgcatccggc ggtgttgccc gcgcacgccg tcccggcgat
     2101 gccgtgggcg ttgaaggcgt tgtagatctc caggcagttc ggcgtgccgt cggtgagatt
     2161 gccgttgtcg tcgtccacgg cgagataggc gctgtaggcg ctctgggctg aactcggcac
     2221 catcgtgtcg ttctgcggca gcgacaggaa gaagagacgc tccgccgtct gccagccggt
     2281 gttgaagccg tgcttcgcca ccagcgcctg cgtcatgtcc cagtgcgtct gtccgtagat
     2341 ctccccctcg cagtggacct cgtggccgaa ggggccgttc tcgcactgtc cggtgcagac
     2401 gaggcagaag gtatcgaggt tgttgacgcg cgcctggtag ccgaccaggt tggaatcgag
     2461 gatccgcacc gacgcgccgg tgatgtcgaa cccggggccg agatgcgggt cgtgcacgac
     2521 cgacatcgag acgatgtcgc cggtcgcctc gccggtcgat gagtcgccgc cgttggtgtt
     2581 gccgtccagg ccgtgccccc actcgtgata catcacgtcg gcgatctcgc cggtgttgtt
     2641 gcagccgccg ccgctccgga agaaattggc ggagccgttc cagaaggcgt tgcagacgtc
     2701 cgggatgttg acgttgatgc ggatggtcgt cccgagccag gggatcgcca gccatttcga
     2761 cgccaggctc cggacgcggt tgagatggaa aaacgagttc cgctcggccg gcgtcgaggc
     2821 gccgttgccg atgacgtcga tccctcccgt cccgaggctc agatcgccgg tcccgcgatc
     2881 gacgtacgcg aaggcgcgca acgggttcga gcagccgacg caagacggcc ggaagaaggg
     2941 cccgtcgagc gacgtgaagg cgggccctcc cggataggcg aaggtgcctc cctcgccggt
     3001 ggcgacctgg ccggcggtgg tcgtgacctg cacattggtg aacggccgca ccacctcggg
     3061 atcggtgacg gtacgcgggt ggacgccgcc ggtgacgcgc gcgtagcggt tgcggtcttc
     3121 gaaggccagc acctcgccgg tgcgggcgtc gatgtcgccg ctccaggtca tgggttccaa
     3181 ggctcgctgg aacgcggcgc gccagaccag gtgccatctc agcccctccg cccacggcac
     3241 gacggcggcg ccgaccggcc gggcgacgta gaggaggtgc ggttcctcga cgacgacatc
     3301 gtcgtcgcgg cggcccaggg cgtgctcgaa gaggagcgcc agcgcctcgt cggcgtcgat
     3361 cgccggcgtg gccttgggga gacctgcgcc gatccgggcg ctgccggcct ggatgatgtt
     3421 gccatggcgc acgcgcagaa agacgcgccc cccctcgacc gggaggccgt ccacgagcca
     3481 gtcgtagtcg aggtagacga gcgcgccatc gtcgagccag gcgctgcgat cggtgttgag
     3541 gcgcagctcc ccttcgcgcg ggcgcaggaa ttcccctccg gcgtcgagga aggcgcggcc
     3601 gaggggctcg atgtcctcga gccgggcggg cgctttcgcg agccccgccg agaccgcggt
     3661 cagcccgttg ccccggcccg gaaagagcgg gatccctgcc ccttccacga gcgacggacg
     3721 tccggtgcgc cggtcgaacg agatggtcca gggcccggga tgccgccgcc ggaaggcggc
     3781 ggccgacgcc gcggccgggc cggccccccc ctcctcctcc gcctccggaa tcggcaggac
     3841 atagggggcg gggatgacgc gccggtcgag gtcctcctgg gggcgaggca cgagcgcctt
     3901 cgcctcgggg gccaggacgg ccaggatcac aagggacaga agtgcgaaac ggatctgcag
     3961 ccggacggca tggcgtgaca tgcgcgtgct ctctcctcag gacggccggg gaccgtgtcc
     4021 cgaagggacc ggagacagga atgtaccggg accatcctcc cggagcaaca actattgaag
     4081 acccgaaggg ggcaccgagc ccggaggaac gcccggatag gaggatttga cccgggcgaa
     4141 ccggagcact taaattcatc cggcgatgac ggaccgccgc gcgtctcgct gcctcctcgt
     4201 ctgcctcctc ctctccggca tgagcggcct cgtgtaccag gtcgcctggg tccgatccct
     4261 cgagctcgtc ttcggggcga ccaccttcgc gatcgcgacc gtcctcgcgt cgttcatggg
     4321 gggcctggcc gccgggagcg ccgccgccgg ggccctcgag acgcgcctga ggcgcttcca
     4381 cccggtgcgg ctgtacgcgg cgtgcgaggt cgggatcgcg gtcgctgcgc tgctcgtgcc
     4441 gctggcgttc cggctcctcg ttccgctgtc gcgcgtggtg tgggagacgt tccacgcctc
     4501 gttcgccgcc ttcagcctgc tgcgcttcgt gctgtgcgcg tcggtgctcc tggtgccgac
     4561 cgcgctcatg ggggcgacgc tgccgatcct cagccggttc gtcgcggcgc tgcgccccga
     4621 ggagtcgggc ggcccgggtt cccgggtcgt ggcggacggc gcgccgggcg atcgcggcag
     4681 cgagccggcc cgacgcgtcg gcatgctgtt cgcggtgaac accgccgggg cggtcgtcgg
     4741 ctgcgcgctg gcggggctgg tcctcatgcc cgggctcggg cttctcggca cgcagtgcgc
     4801 cgccgtcgcc ctgaacctgc tcgccgccgg cggggcgatg tgggtggggc gcggccgtcc
     4861 gttcgacgcg gccgccgtgg ccgcggccga tcgtccggcc atggcgccgg aggcgggcgc
     4921 cgtgccggga cgcgcgccgg tgttcatcgc ggcctacgcg atctccggcg ccgtggcgat
     4981 gctgtacgag gtcgcctgga gccgctcgct cgtcctcgtc ctcggctcgt ccacctattc
     5041 ctacacgacg atgctgacga ccttcctggc cgggctgacg atcggggcct ggctcggcac
     5101 gcgggtgctg aaggcgaagg ccgatccgct cctggcgatc gcgctgtgcc agctcatgac
     5161 ggcgacgacg gtggcgctcg gactgttcct ggccggggag ctgccgttcc tctacgtgca
     5221 gctggacgag gcgctcgatc cgtccgcgcg cggcctgctc ttcgtgcagc tggcgctggc
     5281 ggcgctggtc atgttcccgc cgaccctcgg cctcggtgcg atgttcccgc tcaccctcgg
     5341 cggaatcggc atgaccacgg ccgccgcgca gcgcctggtg tcgcgcgcct acgcctggaa
     5401 cacggtcggt gcgatcgccg ggtcgctgct cggcggattc tggctcctgc cgcggctcgg
     5461 gacgcgcgac gtgctgatcc tcgggatcgc cgtcaacgcc gcgatcgcgt tcgccgggat
     5521 cgccctgacg cgcgccggcg agctgcggtc gacgccgcgc gcggcgcttc tcgtcctcat
     5581 cgccgtcttc ggcgtccacc tggccctcgc ggccccggag tggcgcagcg acgtgatgtc
     5641 gagcggggtg ttccgctacg ccgaccggta tcgcggcctc gaccgggccg ggttccgcaa
     5701 gcgggtgcgc cagagccacg gcgacatgct gttcttcgag gaggggctga cctgcaccat
     5761 cagcgtcttc cgcacgacgc gatccctcac gatgctcaac aacggcaagc cggacgcctc
     5821 ggtgccgccg gggctcgcgc tggtcggagc ggcgggcgcg ggtccggacg tcgtggtgga
     5881 cgcgggaggc gccggcgcgg cggcgacggc ggccgtgcgt tccgagccgc tcggcgacct
     5941 gccgacccag gtgctggtcg ggcagatccc gctcctgctc gccgaccgca tcgacgacgt
     6001 gatggtcgtc gggctcggca gcggcgtcac gctgggctcg gtcctgacgc acgacgtgaa
     6061 gaaggtggac accctcgagc tcgaggcggc ggtcgtgcac ggcagccggc tcttcgacca
     6121 ccacagcggc gcgccgctcg aagacccgcg cgtgtcgctg gtggtcaacg acgcgcgtaa
     6181 cgacctgctc gtgcgcgacc gtcgttacga cgtcatcatc tcggagccat ccaacccgtg
     6241 gatcccgggg gcggcgagcc tgttcacccg cgacttcttc cggctcgccc ggcgccgtct
     6301 gcagccgcac ggggtgctct gccagtgggt gcagctgtac gagctgtggc cggaggactt
     6361 ccaggcgatc ctgcggtcgt tcgccgaggt cttcccatcc atccagatct ggcgcgtggg
     6421 ctccgacgcc atcctcgtcg gcggaccacg cgacatcccg ctgccggtcg gccgggtgtt
     6481 cgcccgcgcc tccgaccggg tgcgcggcga cctcgcccgc atcggcctgc gcggcccgga
     6541 ggatctcctg gcgcacttct ggatcggcgg cgacgagctg cgcgccgcga tccctcccgg
     6601 cccgatcaac accgacgaca acatgcggat cgagttcgcg gcgccgctcc ggatgctggc
     6661 ccgcgacaac gagcggctcg agcggcagaa acaggagctt cggggcatgt tcgccgggcg
     6721 gacgagcgcg gccctgccgc acctgcgttt cccggcggac gacccgaccc gcgaaggagc
     6781 gttcctggcg cgcctggccc gggcggcgct cgaacgcggg tacgaagacg aagcggcggt
     6841 gtatgccgcg gcggcgtggc agcgcgcccg cggtcccgag accgcctcga cccgcgccgt
     6901 cgtcctcgcc gtgctcgggc gctccgagga cgccgcggcc gcgcgcgacg aggcggagcg
     6961 cggctggccg gacgatcccg gcgtgcagcg gatgctcctc gacgcggcgg tcgtgaacga
     7021 agagccggag gccatccgcc gccacgcaca ggcggtcctg cgcgcggcgc cggacgacgc
     7081 ttcggcgcgc tacgagctcg cgaaggtgct cgacgcggtg ggggagaacg acgccgcgct
     7141 gcgggcgatc ctgccgctgc gggcgctgtt cgcgccggcg gtggcgaggg catccggcgc
     7201 cccgggaccc gcggcgaccg cgcccgacgg tgcggcgcgg gtcctcggcc tgctgctcgc
     7261 ggccgccggc cgcgccgccg aggcggagcc ggctctccgc gcccatctcg ccgcccatcc
     7321 cgacgatgcc gcggtgctga ccgcgctcgc tggtgcgatg cgggccgccg gacgcgacgc
     7381 ggaggcggcg gccatcgagc gccgcctcga ccccgacgcg aagtcccagg ccgcgacgcg
     7441 cctcgccgcc gcgagccggg ccttcgagtc cggtcgctac gacgccgccc gtaccgcgct
     7501 cgaagaggcg ctgcagttcg cccccgacga ggaagcggcg gccctgctcc tggcgcggac
     7561 gctccagcgg ctgggagaca cgccgggggc tgcggatctc ctgaaaacgt ggctcgcgag
     7621 ccactccgac cgcccgctcg cgttggggct gctctcgcag gtccttctgg atctcggcca
     7681 gccggacggc gccgccgcgg ctgccacgcg ctaccgcgcc ctcaccggcg aagactgggc
     7741 cgggatggat gtcatcgcca gtctcccgtc gcgatgaatg ttgacgggcg ggtgtccgcg
     7801 acgtagagtc gccggcgatg tcgttcaagc ggctctcgac cgagcgcgtc tacgccggac
     7861 gactcctcaa gatcgatcgc gaccgcgtcc tcctgccgaa cggcggaacg accgagctcg
     7921 agatggtgcg gcatccgggg gcggcggcgg tcgtcccgtt catcaccgac caggagatcc
     7981 tgctcgtccg gcagttccgt tacgccgcgc aggggttcat ctacgaggtc cccgccggga
     8041 cgctgcatca gggagaagcg ccggaagcgt gtgcggcacg cgaggtgcag gaggaggtcg
     8101 ggcaccgcgc cgggacgctc cagcatctcg cgtcgatcta cacgaccccg gggttcaccg
     8161 acgaggtgat ccatctctac accgcgcgcg acctcgagcc cgcggcgcag aagctcgacc
     8221 atgacgaggt gctgacggtc gagcggctgc cgttcgccga ggcgcttcgg atgatccgcg
     8281 acgggcggat cgtggacgcc aagagcatct gcgccctgat gctggcccgc ccgtgaacgg
     8341 tcctcatcgc cgtcctgctc ctctgatttg aatagaatgt ccgcccttga gacgctcgag
     8401 gcgcttccca tggacgtcct gaaatcgcgg ctcgacccga aggggtcgga gttccaggaa
     8461 aatgcccggc accaccagcg tctcgccgag gagctgcgga cgcggcacgc gcgcgtcaag
     8521 gaaggcgggc cccggcacgc ggcggcgacg cagaagaagc gcggcaagct gccggtgcgg
     8581 gagcgcgtgg cgcgcctcct cgacgacaac acccctttcc tcgagctgtc cgcgctggcg
     8641 gcgtggggcc ttcacgaagg tgacgccccc ggcgccggcc tcgtcaccgg catc
//
LOCUS       DASRIQ010000501         3539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3592410, whole genome shotgun sequence.
ACCESSION   DASRIQ010000501 DASRIQ010000000
VERSION     DASRIQ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3592410"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="VFD06_12160"
     CDS             <1..1574
                     /locus_tag="VFD06_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN04326.1"
                     /translation="SGAPAVRTPAPAPSGGGRIVIGVRGDVTSFNIYTATNAFTQEVV
                     DLLQLRLAMEQDDFKDHPPTFQPALASSWEFSPDRLNLTFRLDPRARFSDGREVTSAD
                     VLFSHQAATSPDVGWVGSDVKEFIASVSAPDPKTVVYRFTRPYPYQLMDAAEGNILPR
                     HLFEPVPLAEWPKRGFFESAAGSGPYVLAKYERGAFIELRKNPAYLFAPRPRLDTVVF
                     RILPDEATLLNELLSGGIDVMENVPPAAADRVRKSPRLRLVRVPDLSYTFLCWNTERP
                     LFREARVRRALTMAIDREAIIEGLLHGTGRPSAGPALSFLWARDPSIEPLPYDPKAAR
                     RILAEAGFADSNGDGLLDRDGMPFRFTLESNQGSGLRADVVQMVAAQLRPLGIEVTPR
                     VVEFGAFIEAHERHDFDAFVGSWRESTKFDLKSAFHSASRTGGYNYGSYANAELDALI
                     DRARAEADAAKARPLWWKAQRIVAADQPYTFLFERDRLHAVPKRLEGFHPDPRSAYTG
                     LPSWTLPPESTRGGR"
     gene            1571..>3539
                     /locus_tag="VFD06_12165"
     CDS             1571..>3539
                     /locus_tag="VFD06_12165"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04327.1"
                     /translation="MKAAAPIPVLGAKLLPPAPGPFHLRRERLHERLRRGLEGRATAV
                     VAGPGYGKSTLVARFLQEHGGDTVWYSLDPSDRDPAIFFRYLMQGLREHAPEFGERIE
                     WIWRESRTGFRTPEQLADVFIGEAEEGLGGRFVVVLDQVEHLEASPPAARALRRLLTW
                     LPGTLHLVLIGRSLPEVGFKGLEADGQAATIGGDLMRFTSEETRTLLNTTFSLAMPQE
                     AIRRVHERTRGWVTALQLLRQTAHLRPGAAGLPEEVFERTESEIFDWFTERVLEGLPE
                     ESRAFLLGSAPPPTFDPDLLAEVLEDLDARAVLQDLLRRNLFLAPLESRGAHFAYDPL
                     FRDYLLRRLQTDRGREERRALERRYSRAFARRREYPQALGHALAAEDVRGTADLLARH
                     GKGLLRRGLLDLVREACGFLRRAGPGSPVVDDLLGEASRLAGDYAAAIPHFERALAAP
                     PDAGLEGPVRASTLQGLAYSLLETGALPRAAETAGRALAEAGDGDPALLARTLNTLAL
                     VHYRADRHDDALARWQEALARARQAGDEHLTLMIAHNLGLPHAVRGDFRRASECFRIL
                     TAAENTRVGPEEGAAYLNLARIAILRGEWDRGASLLGDAREIAQKLRLPALQADVLEA
                     EGTLLRETGDAAGAAERYERARHEFTGLGRID"
BASE COUNT          461 a         1310 c         1268 g          500 t
ORIGIN      
        1 cctcgggcgc gccggccgtc cgcactccgg cccccgcgcc gtccggcggc gggaggatcg
       61 tcatcggagt gcgcggggat gttacctcct tcaacatcta caccgccacg aacgccttca
      121 cgcaggaggt ggtcgacctc ctgcagctcc gcctcgcgat ggagcaggat gacttcaagg
      181 accatccacc gacgtttcag ccggcgctcg cctcgtcctg ggagttctca ccggaccgcc
      241 tgaacctgac gttccgactc gatccgcggg cgcgcttcag cgacgggcgc gaggtgacct
      301 cggccgacgt gctgttctcg caccaggcgg cgacgagccc cgacgtcggc tgggtgggga
      361 gcgacgtgaa ggagttcatc gcctccgtga gcgccccgga tccgaaaacc gtcgtgtacc
      421 gcttcacccg cccgtaccct taccagttga tggacgcggc ggaagggaac atcctgccgc
      481 ggcacctctt cgagccggtg ccgctggccg agtggccgaa gcgggggttc ttcgagtcgg
      541 ccgcgggcag cggcccctac gtgctcgcga agtacgagcg cggcgccttc atcgagctca
      601 ggaagaatcc cgcctacctg ttcgcgccgc ggccgcgcct cgacacggta gtcttccgga
      661 tcctccccga cgaggcgacc cttctcaacg agctcctctc cggcggcatc gacgtcatgg
      721 agaacgtccc gcccgccgcc gccgatcgcg tccgcaagtc gccgcgcctg cggctcgtcc
      781 gcgtccccga cctgtcgtac acctttctct gctggaacac cgaacggccg ctgttccgcg
      841 aggcgcgtgt gcggcgggcg ctgaccatgg cgatcgaccg cgaggcgatc atcgaggggc
      901 tcctgcacgg caccgggcgt ccgtcggccg ggccggcgct ctcgttcctg tgggcgcgcg
      961 acccgtcgat cgagccgctg ccgtacgacc cgaaggccgc gcgccggatc ctcgccgagg
     1021 cgggcttcgc cgattccaac ggcgacggcc tcctcgaccg ggacgggatg ccgttccgct
     1081 tcaccctcga gtcgaaccag ggatcggggc tgcgggccga cgtcgtccag atggtcgcgg
     1141 cccagctccg cccgctcggg atcgaggtga cgccacgggt ggtcgagttc ggcgccttca
     1201 tcgaagcgca cgagcgccac gacttcgacg ccttcgtcgg atcgtggcgg gaatcgacga
     1261 agttcgatct caagtcggcc ttccacagcg cttcccgcac cgggggctac aactacggct
     1321 cctacgcgaa cgcggagctc gacgcactca tcgaccgggc gcgcgccgag gcggacgccg
     1381 cgaaggcgcg tccgctgtgg tggaaggcgc agcggatcgt cgcggccgac cagccgtaca
     1441 cgttcctctt cgagcgggac cggctgcacg cggtcccgaa gcgtctcgag ggattccatc
     1501 ccgatccgcg cagcgcctac accgggcttc cctcctggac gctgccgccg gaatcgacgc
     1561 ggggggggcg gtgaaggcgg cggcgccgat cccggtcctc ggggcgaagc tcctgccccc
     1621 cgcccccggt ccgttccacc tgcgccgcga acggctgcac gagcgcctgc ggcgcgggct
     1681 cgaggggcgc gccaccgcgg tcgtcgccgg gccgggctac ggcaagtcga cgctcgtggc
     1741 ccgcttcctc caggagcacg gcggggacac cgtctggtac tcgctcgatc cgtccgaccg
     1801 cgacccggcg atcttcttcc gctacctgat gcaggggctc cgcgagcatg ccccggagtt
     1861 cggcgagcgg atcgagtgga tctggaggga gtcgcgcacc ggcttccgca cgccggagca
     1921 gctcgccgac gtgttcatcg gcgaggcgga ggaggggctc ggcggccgct tcgtcgtggt
     1981 cctcgaccag gtggagcacc tcgaggcgag cccgccggcc gcgcgggcgc tgcggcgcct
     2041 gctcacgtgg ctccccggca cgctgcacct ggtcctcatc gggcgctcgc tgccggaggt
     2101 tgggttcaag gggctcgaag cggacgggca ggccgcgacc atcggcgggg acctcatgag
     2161 gttcacctcc gaggagaccc gcaccctgct caatacgact ttcagcctgg cgatgccgca
     2221 ggaggcgatc cgccgggtgc acgagcggac ccgcggctgg gtcacggcgc tgcagctcct
     2281 gcggcagacg gcgcacctgc gcccgggcgc cgccggcctg ccggaggagg tcttcgagcg
     2341 caccgagtcg gagattttcg actggttcac cgagcgggtc ctggaggggc ttcccgagga
     2401 atcgcgcgcc ttcctgctcg gctcggcgcc gccgccgacc ttcgatcccg acctgctcgc
     2461 cgaggtgctg gaggatctcg acgcgcgcgc cgtcctgcaa gatctcctac gccgaaacct
     2521 gttcctcgct ccgctcgaga gccgcggcgc gcatttcgcc tacgacccgc tgttccgcga
     2581 ctacctgctc cgccgcctgc agacggatcg cggccgcgag gagcgccgcg cgctggagcg
     2641 gcgctacagc cgcgccttcg cgcgccgccg cgagtacccc caggcgctcg ggcacgcgct
     2701 cgccgccgag gacgtccgcg gcacggccga tcttctggcc cgccacggga aagggttgct
     2761 gcgccggggc cttctggatc tcgtccgcga ggcgtgcggc ttcctgcgcc gggccggacc
     2821 gggcagcccg gtggtcgacg acctgctcgg agaggcgagc cgtctggcgg gggactacgc
     2881 ggccgcgatc ccgcacttcg agcgggcgct cgcggcgccg cccgacgccg ggctcgaggg
     2941 tccggtgcgc gccagcacgc tgcaggggct cgcctactcc ctgctcgaga ccggtgcgct
     3001 gccgcgggcg gccgagaccg cggggcgggc gctcgcggag gccggggacg gcgatccggc
     3061 gcttctggcc cgcactctga acacgctggc gctggtgcac taccgggccg accggcacga
     3121 cgacgcgctc gcccgctggc aggaggcgct ggcccgggcc cgtcaggcgg gggacgagca
     3181 cctgacgctg atgatcgccc acaacctcgg gctgccgcac gcggtgcgcg gggacttccg
     3241 gcgcgcctcg gagtgcttcc gaatcctcac cgcggccgag aacacccgcg tcggcccgga
     3301 ggagggggcc gcgtatctca acctggcgcg catcgcgatc ctgcggggcg agtgggaccg
     3361 gggcgcgtcg cttctgggag acgcgcgcga gatcgcgcag aagctgcggc tgccggcgct
     3421 ccaggcggac gtcctcgaag cggaggggac gctgctgcgc gagaccggcg acgcggcggg
     3481 ggcggcggag cgctacgagc gcgcccgcca cgagttcacc ggcctcggcc ggatcgatc
//
LOCUS       DASRIQ010000502         9566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_730373, whole genome shotgun sequence.
ACCESSION   DASRIQ010000502 DASRIQ010000000
VERSION     DASRIQ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730373"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFD06_12170"
     CDS             <1..935
                     /locus_tag="VFD06_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN04328.1"
                     /translation="PEILRAKVRAFVELYRKTRQLEALNRELEARVGERTSELEATAA
                     ALQVSDQRKDEFLATLAHELRGPLAPLSNMLEVLKRADDDPALRQKARETMERQLRHL
                     VRLVDDLLDVGRIAGNKLALRTEQVELASIVEQSVETCRPFFEAAGHLLSVSVPAEPI
                     HLIADPVRLTQVLNNLLNNACKYTPPGGRVGLTVWSEGTDALMAVRDTGRGIPAEMMP
                     HIFEMFTQVDRSLERSQGGLGVGLSLVRHLVELHGGAVAARSDGEGRGSEFVVRVPAL
                     PGAAVRAERRSAVVTPWSDEGLRLGPSAPTRTAS"
     gene            complement(949..1413)
                     /locus_tag="VFD06_12175"
     CDS             complement(949..1413)
                     /locus_tag="VFD06_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HZN04329.1"
                     /translation="MWCSGCGGSLQGSRFLTIPIDACGECGGTWLEEGRLKWILEVGP
                     KSLPAEATREATRFKPQERRGSYALAPDEVRRIVKCPYCVGVMRPVNYSATSGVAIYR
                     CLSDHGVWVPKGGLDRLVVFVDTWDRYLKAAAPYYSRLAQLERQRFLKRVEF"
     gene            1926..2948
                     /locus_tag="VFD06_12180"
     CDS             1926..2948
                     /locus_tag="VFD06_12180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN04330.1"
                     /translation="MTVSPLLTRGVRSGALLGALFLLAVVTTGAPGAQEKKPEAPKND
                     APKASLYVHIETRSVLTYAGDPNEVTILFQNDSKENWTNPGMEIESGFQVFDSEGKKL
                     EKVKSPVSLKEAQPKVLEPKGYFGKIVDLNTLFPKMNAIGTYKITWAATGLAEKTIMS
                     RVIKKYDPARDYQAVLETDFGNVVLEFYKDLAPSHVKNFIDLANQGYYDQKIVHRIIK
                     GEAIFGGSRTGDERGGPGYTLTPEPNGLKILAGSVAQVRNSYTGAEESGSIFMVAAVP
                     QPEMDDRYTVFARVAQGLETIKAISNLPTAGGSGGKVAARPIRDVTIKKIEIREKKSD
                     KPAKAS"
     gene            2971..4449
                     /locus_tag="VFD06_12185"
     CDS             2971..4449
                     /locus_tag="VFD06_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HZN04331.1"
                     /translation="MRPAHRGPGSLRAVRPAAAAWLLAAGLVAGAAAFGVATPPTEDF
                     DAPVPEWADGPVRYVLPQEDKLAYRRLETAEERARFIRRFWAARDPDRTTPDNEYRAL
                     FFRRVEEAQRQFTGESTKPGWKTDRGKIWILLGPPDEHDDYAPRSIRPDVINWTYRNP
                     PPGSGAGLNVQVRFVLDASGEYRLSSGARIFSSDTAMSQAFAVQALQQKSLPGIRPPG
                     DVPEGVETAASGLRPHTDVFASGRDRMLAILTLFVPESFLKGDGAAAPLVEVTARLVS
                     ETPGGPTYDLSGPSSLRTAAGPLGGGPGGTRIYQAGLTVRPGTYKVRYALNGPGGATL
                     HSLDDTLVAPLPAPGRLAVGPIAFAGRLERMPEPVASEYVAPFVLGSLRVVPRPDAIF
                     SPGESLSFYYQISGATNDPIGGIPELEIEYVILIEGQGPSGIPSTQPLGHPIHLTREP
                     SLLQSFSLPLDGWSAGRYRLQVHVKDNLNEAVAASEIAFQLR"
     gene            4539..5594
                     /gene="yvcK"
                     /locus_tag="VFD06_12190"
     CDS             4539..5594
                     /gene="yvcK"
                     /locus_tag="VFD06_12190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HZN04332.1"
                     /translation="MSAAAHRSRVPRSASGSPASRRPRGLKVVALGGGTGLPVVLRSL
                     KRAIAASGGTLTGIVTVTDDGGSSGRLRRAYDVLPMGDIRNCLVALSENEPLMSRLFQ
                     YRYRGRDGLGGHTVGNLILTALADSTRSYLRAIETSGEVLAIRGRILPATLRNVALVA
                     RCSDGRMLRGETRVSQSHAPITRIMTDPPQPPPAPGVLQALREADLIILGPGSLFTSV
                     VPNLLVRGVARVIARARAPRVLVGNLMTQPGETDDFTAAAHLAALTRLAGARVVDYYL
                     SNSRRIAPALRRRYAADGAVPVEMDRKALGALGAVGVERDLLREDRQGAKIRHHEGKL
                     GRALLRLALARPSRAAA"
     gene            5639..6193
                     /locus_tag="VFD06_12195"
     CDS             5639..6193
                     /locus_tag="VFD06_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HZN04333.1"
                     /translation="MRLNLSDEPITAELLAEALKKSGSAQILVSGTSMHPTLQMGWRV
                     ILEPATGADLRPGEIAVFRTEHHLTVHRLVWIETGPDGTMLVFRGDYNRVRERVAPEA
                     VLARAVAVEAPSSRRTVGRIETLGGDVLDRFYRLVWILSRPFARVLPRVGGRDVRPGP
                     LGRAARTGFAGIERVLSIFLPRRP"
     gene            6416..7783
                     /locus_tag="VFD06_12200"
     CDS             6416..7783
                     /locus_tag="VFD06_12200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN04334.1"
                     /translation="MKNILVAEDDRALRDGLVQAMSRGGYKVHVAQNGKEAMDKIDKQ
                     VFDLVIAGQRVGGSEGLEIVRRARASNAGTMVIVTGEGGQGAAEAMRSGAFDFVQKTL
                     SLEAIEMKIQRAIEHQRLMTRVSTFAERQSDTGPAPGLMGPSPMMREVFRIIDKVAAS
                     NATVLIQGETGTGKERVAETIHLHSTRRDGPFVRMNCASLPDNLLESELFGHEKGAFT
                     GADQMRTGRFELANDGTLFLDEVGNMSASTQAKVLRAIQNQEFERLGGSRTIKVDVRI
                     VAATNVNLEQAIKEGRFREDLYYRLNVVNIAIPPLRDRREDILPLAEHFLQRFALELR
                     RPVKAFSAEAVKALQEYRWSGNVRELENTVERAVLMCEGEQIRPQDMTLMDREHSIGM
                     TPQLALDLLNLETLEKTAILEALKRSNWVQKEAAKLLGVSSRVMNYKVHKFGITHDRW
                     SKNRN"
     gene            8011..8967
                     /locus_tag="VFD06_12205"
     CDS             8011..8967
                     /locus_tag="VFD06_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013679090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZN04335.1"
                     /translation="MAIYLTENDVDRLLGMDACIEAVERAFVEAAAGRAVFRPRARVA
                     LPQAMLHVLPAGSEKLGRFAVKCYATSRSGARFVVLLFDATTSDLLATIEADRLGQRR
                     TGAASAVATRRLAHREAGILAILGTGWQARSQVEAIARVRKLREIRAWGRDRGRLIDF
                     CGEVAKATSVAVQPAPSAEAAVRGADIVTTATSAAVPILKGAWLSPGMHVNAVGSNRS
                     DRREIDDEAVARADVIVADSVEQARHEAGDLLLAVPPEGALQPVARAIELSAVVAGRH
                     PGRTDDRAITLFKSLGIGLEDLAAASLVFDRAVAAGAGRSWP"
     gene            9171..>9566
                     /locus_tag="VFD06_12210"
     CDS             9171..>9566
                     /locus_tag="VFD06_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04336.1"
                     /translation="MSARALEDQIPRATYRFTLTYQFLPTLSAGIEYNPQVEEVNPLL
                     NWLVLQEKEKRPAVVIGTSSDRIGTPEGQSYYLTVSKNLKELTHLPIAPYIGAAYGTF
                     EDRWRFIGGMEIDFTHGFWALVIYDGVNWH"
BASE COUNT         1456 a         3412 c         3339 g         1359 t
ORIGIN      
        1 tgccggagat cctgcgcgcc aaggtccggg cgttcgtcga gctgtaccgc aagacccggc
       61 agctcgaagc cctgaatagg gagctcgagg cgcgggtcgg cgagcggacc tccgagctcg
      121 aggccaccgc ggcggcgctg caggtctccg accagcgcaa ggacgagttc ctcgcgacgc
      181 tcgcgcacga gctgcgcggg ccgctcgcgc ccctgagcaa catgctcgag gtgctgaagc
      241 gggccgacga cgatcccgcc ttgcgccaga aggcgagaga gacgatggag cggcagctgc
      301 ggcatctcgt gcggctggtg gacgacctgc tcgacgtcgg gcggatcgcc ggcaataagc
      361 tggcactgcg gaccgagcag gtcgagctgg cgtcgatcgt cgagcagtcg gtcgagacgt
      421 gccggccgtt cttcgaggcg gcgggccacc tcctgtccgt gagcgtcccg gcggagccga
      481 tccatctcat cgccgacccg gtgcggctga cccaggtcct gaacaacctg ctcaacaacg
      541 cctgcaagta cacccccccc ggaggccggg tcgggctgac cgtctggagc gaggggaccg
      601 acgcgctgat ggcggtgcgg gacaccggcc ggggcatccc ggccgaaatg atgccgcaca
      661 tcttcgagat gttcacccag gtcgaccgct ccctcgagcg gtcccaggga ggcctaggcg
      721 tcgggctgtc gctggtgcgc cacctcgtcg agctgcacgg cggcgcggtc gcggccagga
      781 gcgacgggga ggggcgcggg agcgagttcg tggtgcgcgt cccggctctg cccggcgccg
      841 ccgtccgcgc cgagcgccga tccgccgtcg tcacgccgtg gtcggacgag ggcctgaggc
      901 tcgggccgtc cgccccgaca cggaccgcgt cctaggacgc gattccgact agaactcgac
      961 ccttttgagg aaccgctggc gctccagctg cgccagccgg ctgtagtagg gggcggcggc
     1021 cttcaggtac cgatcccagg tgtcgacgaa caccacgagc cggtcgagcc cccccttcgg
     1081 cacccagacg ccgtgatcgg aaaggcagcg atagatggcg acgccgctcg tggcggaata
     1141 gttcaccggg cgcatgacgc cgacgcagta ggggcacttg acgatgcggc gaacctcgtc
     1201 gggggcgagg gcataggagc cccgtctttc ctgcggcttg aaccgggtcg cttcacgagt
     1261 ggcctccgcg ggcagcgatt tcggaccgac ctcgaggatc cacttgagac gcccttcctc
     1321 gagccaggtg ccgccgcact ccccgcaggc atcgatcggg atcgtgagaa agcggctgcc
     1381 ctggagactg cccccgcacc ccgagcacca catcggatcc cgctcccccg ccggtcgcgc
     1441 cacacgacac gcgttccggc cgaccccata tacggtcaaa caccgtaccc agcaaggctt
     1501 ttggcggaat ggagcgcgcg acagaaggga tgtcaggaac atgacggccg cgtccacgct
     1561 tcccacctgg cggcatcgag ctcacggccc gaaagcctgc gcccggtcag acccccaact
     1621 cgcaacccgc ctcgcgtctg ctcatgccgg tacggtcgct cgctcggcgg ctcgacgacc
     1681 cctcggtggc ctgcgccttt cgggcctggc atccaagtgc cgacggtggg aagcgcggac
     1741 gctgccgtag tgctcctctc ttccggcgcg cactcccttc tccatcgagg cgttggcggc
     1801 tgcgtgtttg acaggtgggg ggggagtcgg tagcatgttt cgcttttcgg ggcgcggcca
     1861 cgggcagcgc gcgttgtttt cggggtccga ccgttcgacc gaatctcgca ttcgaggtgt
     1921 tgacgatgac cgtaagcccc ctcctcacgc gcggcgtccg ttcgggcgcg cttctgggcg
     1981 ccctgttcct gctcgccgtt gttacgacgg gtgcgccggg agcgcaggag aagaagccgg
     2041 aggcgcccaa gaacgacgcg ccgaaggcat cgctgtacgt gcacatcgag acgcgcagcg
     2101 tcctgaccta cgccggcgat ccgaacgaag tcaccatcct gttccagaac gactccaagg
     2161 agaactggac caaccccggg atggagatcg agtccggctt ccaggtcttc gacagcgagg
     2221 ggaagaagct ggagaaggtg aagagccccg tgtccctgaa ggaggcgcag ccgaaggtcc
     2281 tcgagccgaa gggctacttc ggcaagatcg tcgacctcaa cacgctcttc ccgaagatga
     2341 acgccatcgg cacctacaag atcacctggg cggccaccgg gctcgccgag aagaccatca
     2401 tgtcgcgcgt catcaagaag tacgacccgg cgcgggacta ccaggcggtc ctggagaccg
     2461 acttcggcaa cgtcgtgctc gagttctaca aggacctcgc cccgtcgcac gtcaagaact
     2521 tcatcgacct ggccaaccag gggtactacg accagaagat cgtccaccgc atcatcaagg
     2581 gggaggcgat cttcggcggc tcacggaccg gcgacgagcg cggcgggccg gggtacaccc
     2641 tcacgccgga gccgaacggc ctgaagatcc tggccggatc ggtggcgcag gtgcgcaact
     2701 cgtacaccgg cgcggaggag tcgggcagca tcttcatggt ggcggcggtg ccgcagccgg
     2761 agatggacga ccgctacacc gtcttcgccc gggtcgcgca ggggctcgag acgatcaagg
     2821 cgatctccaa cctgccgacc gccggaggct ccggcggcaa ggtcgcggcg cgcccgatcc
     2881 gcgacgtgac catcaagaag atcgagattc gcgagaagaa gtcggacaag cccgccaagg
     2941 cgtcctgatc gccccgcggg cgcgatcccc atgcgccccg cacaccgcgg ccccggttct
     3001 ctccgggctg tccggcccgc cgccgcggcc tggctgctcg cggcgggact cgtcgcgggg
     3061 gctgccgcct tcggggtggc gactccaccg accgaggatt tcgacgcccc ggtcccggaa
     3121 tgggccgacg gtccggtccg ctacgtcctg ccgcaggagg acaagctcgc gtaccggcgc
     3181 ctcgagaccg ccgaggagag ggcccgcttc atccgccgct tctgggcggc gcgcgatccc
     3241 gaccggacca ctccggacaa cgagtaccgc gcgctgttct ttcgccgggt cgaggaggcg
     3301 cagcggcagt tcacgggcga gtcgaccaag ccggggtgga agaccgatcg gggcaagatc
     3361 tggatcctcc tcggcccccc cgacgagcac gacgactacg cgccgcgatc gatccgcccc
     3421 gacgtgatca actggaccta ccgcaatccc cctcccggca gcggcgccgg cctcaacgtt
     3481 caggtgcgct tcgtcctcga cgccagcggc gagtaccggc tcagcagcgg tgcgcggatc
     3541 ttctcgagcg acacggcgat gtcgcaggcg ttcgccgtgc aggcgctgca gcagaaaagt
     3601 ctccccggga tccggccccc cggcgacgtc ccggaagggg tcgagacggc ggcgtccggg
     3661 ctgcgcccgc acacggacgt cttcgccagc ggccgggacc ggatgctcgc catcctgacc
     3721 ctcttcgtgc cggagtcgtt cctcaagggt gacggcgccg cagccccgct cgtcgaggtc
     3781 acggcgcgcc tcgtcagcga gactccgggc ggcccgacct acgatctgag cggcccgtcg
     3841 tcgctgcgca ccgccgcggg cccgctcggc ggcggcccgg gcgggacgcg catctaccag
     3901 gccggactga cggtccgccc cgggacctac aaggtgcgct acgcgctcaa cggtccggga
     3961 ggcgcgaccc tgcactcgct ggacgacacg ctggtcgcgc ccctcccggc ccccggccgg
     4021 ctggcggtcg ggccgatcgc cttcgcgggg cggctggagc ggatgccgga gccggtggcg
     4081 agcgaatacg tcgcgccgtt cgtgctcggg tcgctgcggg tcgtgccgcg cccggacgcg
     4141 atcttctccc ccggcgagag cctctccttc tactaccaga tctccggcgc gaccaacgat
     4201 cccatcggag ggatcccgga actcgagatc gagtacgtca tcctgatcga gggacagggg
     4261 ccgtccggga tcccctcgac gcagccgctc ggccacccga ttcacctgac gcgcgagccg
     4321 tccctgctgc agtccttctc gctgccgctc gacggctgga gcgcggggcg ctaccgcctg
     4381 caggtccacg tgaaggacaa cctcaacgag gcggtcgcgg cgtcggagat cgccttccag
     4441 ctgcgctagg tcccgggctc ctagctccgc acgtcggttg accccgcccg acgcgctccc
     4501 ctatattccc cgccgtccac accgtaggag ccgctgccgt gagcgccgcc gcgcatcgaa
     4561 gccgtgtccc ccgctctgcc tctgggagcc ccgcctcccg caggccgcgc gggctcaagg
     4621 tcgttgctct cggggggggg accgggcttc cagtcgtatt gcgctcgctc aagcgggcca
     4681 tcgccgcgtc cggcgggacg ctcaccggca tcgtcacggt caccgacgac ggcggctcga
     4741 gcggtcggct gcggcgcgcc tacgacgtcc tgcccatggg agacatccgg aactgtctcg
     4801 tggccctgtc cgagaacgag ccgctcatgt cccgtctctt ccagtaccgc taccgcgggc
     4861 gcgacggtct gggagggcac acggtcggga acctcatcct gacggcgctc gccgactcga
     4921 cccgctcgta tctgagggcc atcgagacct ccggggaggt cctggcgatc cgcggtcgca
     4981 tccttccagc gacccttcgg aacgtcgcgc tcgtggcgcg gtgctccgac ggcaggatgc
     5041 tgcgcggcga gacgcgcgtg tcgcagagcc acgcgccgat cacccggatc atgaccgacc
     5101 cgccacagcc cccgccggcg cccggggtgc tgcaggcgct gcgcgaggcg gacctcatca
     5161 tcctcggtcc cggctcgctg ttcacgagcg tcgtcccgaa cctcctggtg cgcggcgtgg
     5221 cgcgggtgat cgcgcgggcg cgggcgccgc gggtgctcgt cggcaatctc atgacccagc
     5281 cgggggagac cgacgacttc accgccgcgg cccacctcgc cgcgctgacc cggctggccg
     5341 gggcgcgcgt cgtggattac tacctctcca acagccggcg aatcgctccg gcgctccggc
     5401 ggcgctacgc cgccgacggc gccgtgccgg tggagatgga ccgcaaggca ctcggcgccc
     5461 tcggcgcggt gggagtggag cgggaccttc tgcgggagga tcgccagggg gcgaagatcc
     5521 ggcaccacga ggggaagctc ggacgcgcgc tgctgcgcct ggccctcgcg cgcccctcgc
     5581 gggccgccgc ctgacgtcgc ctcacgccgc gaccgtcggc gcgtgacaaa atgcccgcgt
     5641 gcgactgaac ctctcggacg agccgatcac ggccgagctt ctggccgagg ccctgaagaa
     5701 atccggctcc gcgcagatcc tggtgtcggg cacgagcatg cacccgaccc tgcagatggg
     5761 ctggcgcgtg atcctcgagc cggccacggg cgcggacctg cgccccgggg agatcgcggt
     5821 gttccgcacc gagcaccacc tcaccgtgca ccggctcgtc tggatcgaga ccggcccgga
     5881 cgggacgatg ctggtgttcc gcggcgacta caaccgggtg cgcgagcgcg tcgcgccgga
     5941 agcggtcctt gcccgcgccg tcgcggtcga ggcgccgtcc tcccggcgca ccgtggggcg
     6001 catcgagacg ctcggcggcg acgtgctgga ccgcttctac cggctcgtct ggatcctcag
     6061 ccgcccgttc gcgcgcgttc tgccgcgcgt cggcgggcgc gacgtccgcc cgggccccct
     6121 gggccgcgcc gcgcgcacgg gtttcgccgg gatcgagagg gtcctctcga tcttcctgcc
     6181 gcgccgaccc tagcctccca ggcgtccggg accgccatcg cgcggcggat gccgcgcacg
     6241 gacggcacgc ccggcgtcaa gccgccgacg gatcccgtcc ggaatcccgg aggcgacgat
     6301 ctttcccccc gcctttccgc ccgcattcct tgactcgacg atttcgacat ttatattggc
     6361 gcgtggtgac gcggaagatc cctgtcacgc gccggcggcg gaggaaatgg ggtccatgaa
     6421 gaatattctt gtggcggaag acgaccgggc gctccgggac gggctggtgc aggcgatgag
     6481 ccggggaggc tacaaggttc acgtcgccca gaacggcaag gaagccatgg acaagatcga
     6541 caagcaggtc ttcgacctcg tcatcgccgg ccagcgggtc ggcggcagcg aggggctcga
     6601 gatcgtgcgc cgcgcgcgcg cgagcaacgc cgggacgatg gtcatcgtca ccggcgaggg
     6661 tggccagggg gcggccgagg cgatgcgctc cggcgcgttc gacttcgtgc agaagaccct
     6721 gtcgctcgag gcgatcgaga tgaagatcca gcgcgccatc gagcaccagc ggctcatgac
     6781 gcgcgtctcc accttcgccg agcgccagtc ggacaccgga ccggctccgg gcctgatggg
     6841 gccctccccc atgatgcgcg aggtgttccg catcatcgac aaggtggcgg cctcgaacgc
     6901 caccgtcctg atccagggag agaccggcac cggcaaggag cgggtggcgg agacgatcca
     6961 cctgcactcg acccgccgcg acggtccgtt cgtgcggatg aactgcgcgt cgctacccga
     7021 caacctgctg gagagcgagc tgttcggaca cgagaagggg gccttcaccg gcgccgacca
     7081 gatgcgcacc ggccgcttcg agctggccaa cgacggcacg ctcttcctcg atgaagtcgg
     7141 caacatgtcg gccagcacgc aggcgaaggt gctgcgcgcc atccagaacc aggagttcga
     7201 gcgcctcggc ggctcgcgca cgatcaaggt ggacgtgcgc atcgtcgccg ccaccaacgt
     7261 caacctcgag caggcgatca aggaggggcg ctttcgcgag gacctctact accgcctgaa
     7321 cgtcgtcaac atcgccatcc cgccgctccg cgatcggcgc gaggacatcc tgccgctggc
     7381 ggagcacttc ctgcagcgct tcgccctcga gctgcgccgg ccggtcaagg cgttctcggc
     7441 cgaggcggtg aaggcgctcc aggagtaccg ctggtccggc aacgtccgcg agctggagaa
     7501 caccgtcgag cgggcggtgc tgatgtgcga aggggagcag atccgacccc aggacatgac
     7561 gctcatggac cgcgagcact cgatcggcat gacgccgcag ctggcgctcg acctgctgaa
     7621 cctcgagact ctggagaaga ccgccatcct cgaggcgctc aagcgatcga actgggtgca
     7681 gaaggaagcg gccaagctgc tcggtgtgtc gagtcgcgtg atgaattaca aagtccacaa
     7741 gttcgggatc acgcacgatc gctggagcaa gaaccggaac taacgcacca gcgaggcaac
     7801 gcgccgccga aacgcaagcc cgccgggtcc ggggtccggg ctcgctggcc gtcggcgcgt
     7861 ggcatcgccg cggcggttcc tccggtcctt gctcacctct ctcgcagcgt cccgcacccg
     7921 gggcttgccg tcatgcgcgg ctcgaatctg gcggcgccgg gctcttccct ctccacttcg
     7981 agcggggcgt gggctatcct gacggacccc atggcgattt acctcacgga gaacgacgtc
     8041 gaccggctgc tggggatgga tgcctgcatc gaggcggtgg agcgggcgtt cgtcgaggcg
     8101 gcggccggac gggccgtgtt ccggccgcgg gcgcgggtgg ccctgccgca ggcgatgctg
     8161 cacgtcctgc cggcgggctc ggagaagctc gggcggttcg cggtcaagtg ttacgcgacc
     8221 tcgcgctcgg gggcccggtt cgtcgtgctc ctgttcgacg cgacgacgtc cgacctgctc
     8281 gcgacgatcg aagcggaccg gctcgggcag cggcgcaccg gggcggccag cgccgtggcg
     8341 acgcgccgcc tggcccaccg ggaggcgggc atcctcgcca tcctggggac cggctggcag
     8401 gcccgttccc aggtggaggc gatcgcccgg gttcgcaagc tccgggagat ccgcgcctgg
     8461 ggccgggacc ggggccgcct catcgacttc tgcggtgagg tggcgaaggc gacgtcggtc
     8521 gcagtgcagc cggcgccgtc cgccgaggcg gcggtccgcg gcgccgacat cgtgacgacc
     8581 gcgacgagcg cggcggtgcc gatcctgaag ggggcctggc tctcccccgg catgcacgtc
     8641 aacgccgtcg gcagcaaccg ctccgatcgg cgggagatcg acgacgaggc ggtggcgcgg
     8701 gccgacgtga tcgtcgccga ctcggtggag caggcgcggc acgaggccgg cgatctcctg
     8761 ctcgcggtcc cgcccgaggg ggcgctgcag ccggtcgccc gcgccatcga gctgtcggcg
     8821 gtcgtggccg gacgccatcc cgggcggacc gacgatcgcg ccatcaccct cttcaagtcg
     8881 ctcggcatcg ggctcgagga cctcgccgcg gcgtcgctgg tcttcgatcg cgccgtcgcg
     8941 gccggcgcgg gcaggtcctg gccctgacgc cgccgcgctg gctgcggatc gccgccggtc
     9001 tcctggccgc catcctgccc gccgccgcga tcgacgcggg cgttccggtg cgcggcgaag
     9061 agggcggtca cgacaaggag ggggtgtcgc gcgtcccctc ctgacccctc tgacccggcg
     9121 aggggcgggc ggtccggggg accgccgagg cgatggagga ccggtggacg gtgagcgccc
     9181 gggcgctcga ggaccagatc ccgcgcgcca cctaccgttt caccctgacg taccagttcc
     9241 tgccgaccct gagcgccggg atcgaataca acccgcaggt cgaggaggtg aacccgctcc
     9301 tgaactggct ggtcctccag gaaaaggaga agcggccggc cgtcgtcatc ggaaccagca
     9361 gtgaccgcat cggcaccccc gaagggcagt cgtactacct gaccgtgagc aaaaacctga
     9421 aggagctcac gcacctgccg atcgccccgt acatcggcgc ggcctacggc accttcgagg
     9481 accgctggcg gttcatcggc gggatggaga tcgacttcac gcacggattc tgggcgctgg
     9541 tgatctacga cggcgtcaac tggcac
//
LOCUS       DASRIQ010000503        16523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3067097, whole genome shotgun sequence.
ACCESSION   DASRIQ010000503 DASRIQ010000000
VERSION     DASRIQ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 16523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16523
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3067097"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1433)
                     /locus_tag="VFD06_12215"
     CDS             complement(180..1433)
                     /locus_tag="VFD06_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN04337.1"
                     /translation="MNANARPKARRRRPLLWFGLPLLGVALVVALFFIKRGDAKTRNG
                     MTGEDLDIRLGRSEVADIQVAVNEVGTIEPVVKVDVKSTLSGEVTDLLVREGDRVSRG
                     QVLARVEPDVNQAQTLSQVRSALNMAEINERDAQRNFEMNQRLFEEGLLSDQILRDYK
                     LRLDTALEDLDAAKTKMRIVVESGVPLDRPISTTQRINIVSPMDGYVIHKNVEIGQTV
                     MSGVSSINEGTAIYTVADVGDMLIKASVNEVDIGRVKLNAPVIITVDAFPYRKFDGRV
                     SHISPAAHLKDKIKVFDTEVTLKEQVPDFRAGMTANIEVRGERVNQAMSVPVEAIFKK
                     EGKEVVYVLKSTFDEPKKGEKAPRKTKSEKYDVSEIWQRFFEERPVRVGVVSLERAQI
                     LDGVQKDLELALENPTRPRQVDDES"
     gene            1707..3272
                     /locus_tag="VFD06_12220"
     CDS             1707..3272
                     /locus_tag="VFD06_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZN04338.1"
                     /translation="MLARLHGFTLLGIEAIPLTIECDISGGLPGIHTVGLPDAAVRES
                     AERIRSALRHSGCDLPARRITISLAPADLRKQGSALDLPIALGIVVAAGQAPPGRLHE
                     ILVAGELGLDGEVRPVRGVLTAALLAHRMALREIVVPRANAAEAAAVAGPGVLAVQTL
                     GEAIALLRGECPPGSTLPTAAAAPVGIAPDLADVRGQAHARRALEVAAAGGHHLVMIG
                     PPGCGKSMLARRLPGILPPLLPEEAIEVSSIHSAAGLLPFGRLVERRPFRAPHHTISG
                     IGLVGGGRGPRPGEITLAHQGVLFLDELPEFGRATLDVLRQPMEQGEVLITRENRTLR
                     MPARSMFVAAMNPCVCGKWGSEDRPCGCTPAMVSRYRERVSGPLFDRFDLQIGLPSVS
                     AREMRKGTAGDRSEEVARRVAAARGRQRERFGSPLAVNATMGPEALRRHVDPGPAGLR
                     LLETAMVRLGLSARSHDAVLRVARTIADLGEKEGVSAEHVAEAIQYRNLDRFMVTGTS
                     QGGPLGTPLTGGA"
     gene            3392..3760
                     /locus_tag="VFD06_12225"
     CDS             3392..3760
                     /locus_tag="VFD06_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN04339.1"
                     /translation="MKALFWLKRHGRCRIQRKTLGDWQVIEVSGRFAAGESEAKFHQE
                     VDSALGSGARRVVVDLTRSHQSDESIAQAAPAAYHRARAKGADMRFVVMPGRAGGFYH
                     MAGLELAIPTYTRLGGAIEF"
     gene            3947..4399
                     /locus_tag="VFD06_12230"
     CDS             3947..4399
                     /locus_tag="VFD06_12230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN04340.1"
                     /translation="MGQTVKLKLSSRITYVDLVHQMTEDLARRAHFPKPAALDLALAV
                     REAFINAVKHGNAMDEGKSVEVEFERNASRFLVAIRDEGNGFDPSQTADPRTNENLER
                     TSGRGIFFMKSFVDEVSFQRRRGRGMEVMLVKHLAPAGRSTEEPGRNQ"
     gene            4396..4731
                     /locus_tag="VFD06_12235"
     CDS             4396..4731
                     /locus_tag="VFD06_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN04341.1"
                     /translation="MKASVRHHGKVTVVDLSGKITIGDGDIKLRETVNALLDEGRKAV
                     ILNLNGVSYMDSAGIGELVACYKRAAEKGAKLKLLNPGGKVQDLLILTRLQEIFEIYR
                     DEREALTSF"
     gene            complement(4826..7468)
                     /gene="mutS"
                     /locus_tag="VFD06_12240"
     CDS             complement(4826..7468)
                     /gene="mutS"
                     /locus_tag="VFD06_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZN04342.1"
                     /translation="MDALTPMLRQYRQVKAEHPDAILMFRLGDFYEMFFDDALVASKA
                     LALTLTARGRGTASEAPMCGVPYHAADGYIARLVNEGHRVAICDQVEDPKQAKGIVRR
                     EVTRVVSPGTLTDPAHLPAREPSFIAAVAFGPDDEFGCALLDLSTGEFRISEHRGRDA
                     RTSLVEHVAAFAPREIVVRDGEETPAWLWGDRDGPPRLTVVPSWRFARDGARSTLLKR
                     FGTASLEGFGCEGKDLAIAAAGALLAHLQDTQKTDLRHVVRLAWHESGDTMSLDAATR
                     RTLEIVENARDGGRDGTLLEVVDRTLTSAGGRMLRDWLLRPLVQVEAIEARLEAVAEL
                     SEKAAERQATGECLDRVHDLERLLARAALGTANARDLVALRDSLAVLPGLAARAAALT
                     SPLLTRLLGGLDALPDLHDLLVRALADEPPIGLREAGLLREGFDAEADGLRALRRDSQ
                     AVLVDIETRERARTGIASLKVRYNRVFGYYLEVGNAHRARVPADYERKQTLVGAERYI
                     TPELKDLEHRVLTAAERLAEIEYAAFVRVRDAVAAEAPRIKATASVVATVDALRSLAE
                     VAKEQGWTRPRLDATTRLAIADGRHPVVERRLSGERFVPNDCHLDTAERQILILTGPN
                     MGGKSTYLRQVALIVLLAQSGSFVPAAEASIGLVDRIFSRVGASDNLARGQSTFLVEM
                     NETANILNNATPRSLLLLDEVGRGTSTFDGLSIAWSVAEHLHDQPAVAARTLFATHYH
                     ELTELALTKPRVRNMKMLVREWNDRIVFLRKVVEGAADRSYGIQVARLAGLPEPVLAR
                     AREVLANLEREELEGDGRPKLARHGDAGPGSPGSRSTSPEQLGLFSPGEHPVIKELHD
                     LPVEGLTPLEALNLLASLQRRLRD"
     gene            7547..7927
                     /locus_tag="VFD06_12245"
     CDS             7547..7927
                     /locus_tag="VFD06_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04343.1"
                     /translation="MDPEPLPVEESPVAPDDLDEEDKVMLVFSYLWVLALVPFMVSRR
                     EYVRWHARQGLLLTGVAGVVFFGIIVVGAILSTVTKVFAWVFGFALLNLIFLYMALAL
                     VCMIKAMRGERWAIPFLGDLVERF"
     gene            7935..8540
                     /locus_tag="VFD06_12250"
     CDS             7935..8540
                     /locus_tag="VFD06_12250"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZN04344.1"
                     /translation="MLDSIHQTLLANPWAGLLLVAALAWLEYVFPPSPGDSAMLLACF
                     LAGAGSLPAVGTYAACLLGSVAGALTAYALGARLGRSYFFLRSEWAAGELQRFERGLA
                     RFGPRLLLVNRFLPGVRGVFLYGAGIGKLPFRPVLVYSSVGNLLWVALIAWAGTSLGD
                     SWEEVQGIFRRYVWGIGIALGIYVTVGFVRARRRARLRTSS"
     gene            complement(8524..9792)
                     /locus_tag="VFD06_12255"
     CDS             complement(8524..9792)
                     /locus_tag="VFD06_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04345.1"
                     /translation="MRRSSLLIVVILVVAGLSCITWVGAGTAAVRESLGGKTVVLPPG
                     PHLRVPLYHRVYRYDTTPFTLDEALPIVTRDQATFKLPCRIVARVSPGDVMTFHAGRS
                     GRDTATYLEETVRGAILSAARARTSDDLFLPASVAALGQQVSSDLISRGISDEGLTIG
                     RPGPQVLLNVVMDNLRRQYPATARKLAEAALKEDPHQALFHAAMGSVLEAEGNPGGAE
                     GQYLEALYLDPTALEPMSRLFVLSMRKGDPQTVGRLERLLIASLQKKKDSPVHHDWLG
                     QVAMRMGQADKAELAFTTAINLAPKTPEFRVSLGTLRVQQKRYDDARAAYEEALKLKP
                     DHALALYNLGVVAAMQNRIDEAIGFFERAAAAAPPSVALLNSMAQAYEQKGDMPRAAE
                     ALRRSLKDRPDQPDRVAALRRIEAGLRKKS"
     gene            9809..10504
                     /locus_tag="VFD06_12260"
     CDS             9809..10504
                     /locus_tag="VFD06_12260"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HZN04346.1"
                     /translation="MRAIRACQRIGALLAAVAVAALAGPGWAAEAREEAPKPVPSATA
                     AARRLEARLAGVKAVEADFTQTLDTPALNEPQVETGRLFLERPGKMRWDYTRPAGKLA
                     LADGRDTWLWLPEDRVAITVPLEGDERDSGISLLMHDRPDLLGRFNVDWGPAFKGERP
                     LRLRPRRAGGPYDALLVTLDESGFPREIVVIDPLGGRLTYRLSSPRFVDRLDPTLFTF
                     TPPPGATVQRATP"
     gene            10615..11106
                     /locus_tag="VFD06_12265"
     CDS             10615..11106
                     /locus_tag="VFD06_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZN04347.1"
                     /translation="MASENSFDIVSKADLQEIDNAVNQTLREIGQRWDFKNTKVTLTR
                     EAQVLTLHAEDESRRRAVIEILSERLASRKVPIKALQFKDAQPAAVGSVRQEIHVQAG
                     IAQDQAKEIVKVLKASGLKVQPAIQGDSVRVRGKNKDDLQAAMRVLRTGTFEFDMQFT
                     NYR"
     gene            11112..11768
                     /locus_tag="VFD06_12270"
     CDS             11112..11768
                     /locus_tag="VFD06_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04348.1"
                     /translation="MSRRAGIGIALVALLAASPSAGAASPDPAVVVKGSLTEANGAPA
                     PFQTVRLIKTRKIRSVSDPKARAQEVEEARTRTDASGAFRFEIPADPSFPFWFVRFYD
                     PNEFDAVKYKIPSDTDVSRAVHDGFGIEVSAVLHFHPDWPKVKALVDQYGPGSHRGQI
                     LRSLGLPTRQEPRDEGKELWVYAASGVSYLFDGDKVLETRRGNASSESAQPAEKVDPQ
                     "
     gene            11765..12811
                     /locus_tag="VFD06_12275"
     CDS             11765..12811
                     /locus_tag="VFD06_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZN04349.1"
                     /translation="MTDLATGLRTPSAADGPSEAPRYPPELPAIIALVADKLLRVEEE
                     CRRNLRSEVGAIDELGRYLADGGGKRIRPILLLLATQMCGYKGDRDVLFASVFEFIHT
                     ATLVHDDVIDEAETRRGRGTMNARWGNGLTVLLGDYLYIKSMNMALEADDIRIIRILA
                     QITLKMIEGEIVADRKRGQIGMTEEEHLEVIRRKTAYLFAGCGRVAGVLSGLEPRRLE
                     SIADYGMNLGMAFQIADDLLDVTADSRTLGKPAASDLKEGKLTLPLIYLLETASGSER
                     EMVRTVLEEGEFLTVSRDEILALLRRHRTLERTRELAAGYALKARQALDLFPPSPARD
                     ALLFLPQFVVSRQY"
     gene            12966..>16523
                     /locus_tag="VFD06_12280"
     CDS             12966..>16523
                     /locus_tag="VFD06_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN04350.1"
                     /translation="MARFLKLERMEIHGFKSFYGRTRFDFPEGITAVVGPNGCGKSNI
                     GDAISWVLGEQRASSLRSESMEDVIFNGSQGKKPLGMAEVSLHFKNIRARVGDTACGT
                     LDGEPGVAPEAMMDPLGDAAAALATPGNGNGQGNGNGHGHGNGHAVHAAVEAEPGAPI
                     EVLSADVAVSEVAPDPGTEAAEAGAPTEGDTGTQAARFFLEELPDEVVVTRRLYRSGE
                     SEYALNGQRCRLRDVQDLLSRTEIGTRLYTTIEQGKIDQILLAKPKERRVIIEEAAGI
                     LGYKTKRRHTEQKLEATQANLLRLADIALEVDKQIQSLRRQAAKARRYRRLMDALRDR
                     RASLAWRKLIDCDAGLEASARALGELGEREAAAAGELGRAEADLEALRLKLDAAEEEA
                     RCRRDALHALDLELDRIQERQRAGAEQAVDLERRASEAQEEIGTLQARADERETAARA
                     LTEDLEREAERVRAAEAGLAGLEADRSARAAAIARCEEELEGLRRRFLDRLDRLAETG
                     RRRAALEEQGRAALVALERLARDRADAERAGEEIERTLVLLEAETTARQGRVQECQRL
                     HQDEGTARRGAEESLAEAELRLEERRGRYAALSERLEALADLERRHAGMAEGVVDLLR
                     GGGGFEPRGVVGETLDVPPGLERAVAAALGPLQDGLAVGAVEEAARGARLLRQRGTGR
                     AAFVPAGAGRVAAASRPPEGIPGLLGVLAERVGGLEPGGVLEARLSRILLMSDLDAAV
                     AAAAHLPGWSLVTPEGDLLDSDGALYGGDGPEQQHGVLARRAERAGLAKQIEEIDAER
                     AEAERDCEALKRGTEERRARAAAADAALQEEQRGLFQSDHARQQKDAERGRLLGTLPL
                     LESEAGRLEREAAARAEEAARAVAEHQEHETERLAAEEAIRGATEGIALMRGGLDEAQ
                     GRASEARAAVAAGHQRLAALARERESVREAERELRTRLAERGQQRQAAHERLAEIARQ
                     ASDLTGQRERTAATRAESAAQDEAALAGLAYDRSLFHAREQTAKESRTLLDAARSERQ
                     EQEIRRARLSSDIEHLEASCREDLGLTLEELRANPPALEEGRTLADDEKDVAEGRASI
                     DSIGPVNLMAIEQCTELEERHGLMEKERQDLEAAIESLRDTIRRINRESRQRFLVAFE
                     AIKAGFEASFTVLFGGGRAELRLQEGEEDVLEAGIEINAQ"
BASE COUNT         2429 a         5714 c         5977 g         2403 t
ORIGIN      
        1 cgatgatggc gatcgactcg ccggggaaga tctccaggtt cacgccgcgc agcgcgtgca
       61 cggccgagtc cccggcgtcg taggtcttgt ggacgtcctg gaggttgatg accggaatcg
      121 atcgatcgaa gggatcgccg cccgcggacg gggagggggt cgtcgccatg cgctcctccc
      181 tacgactcgt cgtcgacctg gcgcggccgc gtcgggttct cgagcgccag ctccaggtcc
      241 ttctggacgc cgtcgaggat ctgcgcccgc tcgaggctga cgaccccgac cctgaccgga
      301 cgctcctcga agaagcgctg ccagatctcc gacacgtcgt acttctccga cttggtcttg
      361 cgcggcgcct tctccccctt cttcggctcg tcgaaggtgg acttgaggac gtacacgacc
      421 tccttgccct ccttcttgaa gatcgcctcc accggcaccg acatcgcctg gttgacgcgc
      481 tcgccgcgca cctcgatgtt ggcggtcata ccggcgcgga agtcggggac ctgctccttc
      541 agggtcacct cggtgtcgaa aaccttgatc ttgtccttga ggtgagcagc gggggagatg
      601 tggctcaccc tcccgtcgaa cttgcggtac gggaaggcgt ccacggtgat gatcaccggg
      661 gcgttcagct tcacgcggcc gatgtccacc tcgttgacgc tcgccttgat cagcatgtcg
      721 ccgacgtcgg cgaccgtgta gatggcggtc ccctcgttga tcgacgacac gcccgacatc
      781 accgtctggc cgatctcgac gttcttgtgg atgacgtagc cgtccatcgg ggagacgatg
      841 ttgatccgct gggtggtcga gatcggacgg tcgagcggca cgccactctc cacgacgatg
      901 cgcatcttcg ttttcgcggc gtcgaggtcc tcgagcgccg tgtcgagccg cagcttgtag
      961 tcccggagga tctggtcgga gaggagcccc tcctcgaaca gcctctggtt catctcgaag
     1021 ttgcgctggg catcccgctc gttgatctcg gccatgttga gggccgagcg gacctgggac
     1081 agggtctggg cctggttgac gtccggctcg acgcgcgcca ggacctgccc cctggagacc
     1141 cggtccccct cgcgcaccag caggtcggtg acctccccgg agagggtcga cttgacgtcc
     1201 accttgacca ccggctcgat ggtgccgacc tcgttgactg ccacctggat gtcggcgact
     1261 tccgaccgcc ccagccggat gtccagatcc tccccggtca tgccattgcg ggtcttggcg
     1321 tcgccgcgct tgatgaagaa aagagcgacg accaacgcta ccccgagcag cggcaggccg
     1381 aaccacagaa gcgggcggcg tcgacgggct ttgggacggg cattcgcgtt catgactcgg
     1441 tctccagggc ggtggcctcg gcggtcggat gtctcgggga aaccgcagta taacccgtaa
     1501 gttgcgaccc ccgacgttcc gttctcaccc gggcattacg accgattcgg accgcaagtt
     1561 cccgcattcg ccggccatgt ccacgggcgg gctgccgtca cgaatttgtt gcccgacgac
     1621 ggtgcagccc ctagattggc ctcccgcgtc tggagagccg cgcacggcgg aaaagcgcct
     1681 cggcgccgac cgcttcgagg cgctccatgc tcgcgaggct ccacggattc acccttctgg
     1741 ggatcgaagc gatccccctc accatcgagt gcgacatcag tggcggtctt ccggggatcc
     1801 acaccgtcgg gctgccggac gccgcggtgc gggaaagcgc cgagcggatc cgatcggcgc
     1861 tgcgtcactc cggctgcgac ctgccggcgc ggcgcatcac catcagcctg gccccggccg
     1921 acctgcgcaa gcaggggtcg gcgctcgatc tgccgatcgc gctcgggatc gtggtcgcgg
     1981 cgggccaggc gccgccgggg agactgcacg agatcctcgt cgccggggag ctggggctcg
     2041 atggagaggt ccggccggtg cgcggcgtcc tcaccgccgc cctcctggcg caccggatgg
     2101 ccctgcgcga gatcgtcgtg ccgcgggcga acgccgccga ggcggcggcg gtggcggggc
     2161 cgggggtgct ggcggtgcaa accctcggcg aggcgatcgc cttgctgcgc ggcgagtgcc
     2221 cgcccgggtc cacgcttccc accgccgccg cggcgccggt cgggatcgcc ccggacctcg
     2281 ccgacgtccg gggacaggca cacgcccggc gggcgctcga agtcgccgcc gccggcggcc
     2341 atcatctggt gatgatcggc ccgccgggat gcggcaagag catgctggcg cgccggctgc
     2401 ccggcatcct tccgccgctt ctcccagagg aggcgatcga ggtgagcagc atccacagcg
     2461 ccgcggggct cctgcctttc gggcgtctcg tggagcggcg gcctttccgg gcgccgcacc
     2521 acaccattag cggcatcggc ctcgtcgggg gcggacgggg accgcgcccg ggggagatca
     2581 ctctggcgca ccagggggtg ctgttcctcg acgagctccc ggagttcggg cgggccacgc
     2641 tcgacgtcct gcggcagccg atggagcagg gagaggtgct catcaccagg gagaaccgga
     2701 ccctccggat gccggcccga tccatgttcg tggcggcgat gaacccgtgc gtctgcggca
     2761 agtgggggag cgaggaccgt ccctgcggct gcaccccggc gatggtctcc cgctaccggg
     2821 aacgggtgtc cgggccgctg ttcgaccgct tcgatcttca gattgggctg ccttcggtct
     2881 cggcgcggga gatgcgcaaa gggacggcgg gggatcggag cgaggaggtc gcgcggcggg
     2941 tggcggcggc gcgcggaagg cagcgggagc gcttcggaag cccgctcgcc gtgaacgcca
     3001 cgatggggcc cgaggcgctg aggcggcacg tcgacccagg cccggccggg ctccggctcc
     3061 tggaaacagc catggtccgg ctggggcttt ccgcccgatc ccacgacgcc gtgctgcggg
     3121 tcgcccggac gatcgccgat ctaggcgaga aggaaggggt gtcggccgag cacgtcgccg
     3181 aggcgattca gtacagaaac ttggatcgat tcatggtgac cgggacctcg caaggcgggc
     3241 cgctgggcac cccgctcacc gggggggctt gacgtccttt cgaggggggg tgtagaagaa
     3301 tcgagtctgc cgcctttcag ggtcccaggc tcttcaggaa tcacctcgtt gccgttcgcc
     3361 gtatggggat gtttctcaag agggggatgc gatgaaggca ctcttctggc tcaagcgtca
     3421 cggtcgctgc aggatccaac gtaagacgct gggggattgg caggtcatcg aggtctccgg
     3481 ccgattcgcg gccggggagt ccgaggcgaa gttccaccag gaggtggatt ccgcccttgg
     3541 atccggcgcc cgccgggtcg tcgtcgatct cacccgctcg caccagtcgg acgagagcat
     3601 tgcccaggcc gcccccgccg cgtatcaccg cgcaagagcc aagggggcgg acatgcgctt
     3661 cgtggtgatg ccgggccggg ccggcggctt ctaccacatg gccggcctgg agctggccat
     3721 cccgacctac acgaggctcg gcggcgccat cgagttctag gccgcgcggt tattaggttg
     3781 aaatcccgtc ctcccacgcc gaggacgcga gtccgtgtgc gggccaattg acactacgat
     3841 aaccgtttgg ctacgatccc gcgacgctcc gacacagcgc gttgacgttg gccgtacggc
     3901 ggggagggat ctccgcgtgc gtccggccac acggtggaga ggctcggtgg ggcaaacggt
     3961 caagctgaag ctcagcagcc gcatcaccta cgtcgacctc gtccaccaga tgaccgagga
     4021 tctggcgcgc cgggcgcact tcccgaagcc ggcggcgctc gacctggcgc tggcggtgcg
     4081 cgaggcgttc atcaacgccg tcaagcacgg caacgccatg gacgaaggga aatcggtcga
     4141 ggtggaattc gagcgcaatg ccagccgctt cctcgtcgcc atccgcgacg aggggaacgg
     4201 tttcgacccg agccagacgg ccgatccgcg caccaacgag aacctggaac ggacgagcgg
     4261 gcgcgggatc ttcttcatga agagcttcgt ggacgaagtt tccttccagc gccggcgggg
     4321 ccggggcatg gaggtcatgc tcgtgaagca cctggcaccg gcgggtcgaa gcacggagga
     4381 acctgggagg aaccaatgaa ggcgagtgtc cggcatcacg gcaaggtgac ggtggtcgac
     4441 ctgtccggga aaatcaccat cggcgacggc gacatcaagc tgcgcgagac ggtgaacgcc
     4501 cttctggacg aggggcgcaa ggcggtcatc ctgaatctca acggggtgtc gtacatggac
     4561 agcgccggaa tcggggagct ggtcgcctgc tacaagcggg ccgccgagaa gggggcgaag
     4621 ctcaagctcc tgaacccggg cggcaaggtg caggacctgc tgatcctgac gcgcctccag
     4681 gagatcttcg agatctaccg cgacgagcgg gaagcgctca cgagcttcta gcctcttcag
     4741 tcaccgcctc ggaaccgcca accgtgactc gttctcgtga tcgcgccgtg acgacgacac
     4801 gatgtgcgac actggcgttt cggtcttagt ctcgaaggcg gcgctgcagc gaagcgagga
     4861 ggttcagcgc ctcgaggggc gtcaggcctt cgaccgggag atcgtgcagc tccttgatca
     4921 ccgggtgctc gccgggcgag aagagcccga gctgctcggg agaggtggag cgcgaaccgg
     4981 gtgagccggg tccggcatcg ccgtggcgcg ccagcttcgg acggccgtcc ccctccagct
     5041 cctcccgctc gaggttcgcc agcacctcgc gcgcccgcgc caggaccggt tccggcagtc
     5101 cggcgaggcg cgccacctgg atgccgtagg agcggtcggc ggccccctcg accaccttgc
     5161 gcaggaagac gatccgatcg ttccactccc gcaccagcat cttcatgttg cgcactcgtg
     5221 gcttcgtgag ggccagctcg gtcagctcgt ggtagtgggt ggcgaacagc gtgcgggccg
     5281 ccaccgccgg ctgatcgtgc aggtgctccg ccaccgacca ggcgatcgac aggccgtcga
     5341 aggtggaggt gccgcgtccc acctcgtcga ggaggagcag cgagcgcggc gtggcgttgt
     5401 tgaggatgtt ggcggtctcg ttcatctcca cgaggaaggt gctctggccg cgcgccaggt
     5461 tgtccgaggc gccgacgcgg ctgaagatcc gatcgacgag cccgatcgag gcctccgccg
     5521 ccggcacgaa ggagccgctc tgggcaagca ggacgatgag cgccacctgc cggaggtagg
     5581 tcgacttccc ccccatgttc ggccccgtca ggatgaggat ctgccgctcc gccgtgtcga
     5641 ggtggcagtc gttcgggacg aagcgctcgc ccgagaggcg ccgctcgacc accgggtggc
     5701 gtccgtcggc gatcgccagg cgcgtcgtcg cgtccaggcg cggccgggtc cacccctgct
     5761 ccttcgccac ctccgcgagc gagcgcagcg cgtccacggt ggcgacgacc gaggcggtcg
     5821 ccttgatgcg cggcgcctcg gcggcgaccg cgtcccgcac ccgcacgaag gcggcgtact
     5881 cgatctccgc caggcgctcc gccgcggtca ggacgcggtg ctcgaggtcc ttcagctccg
     5941 gcgtgatgta gcgctcggcg cccacgaggg tctgcttgcg ctcgtagtcg gcggggaccc
     6001 gggcgcgatg ggcgttcccc acctcgaggt agtagccgaa gacccggttg tagcgcacct
     6061 tgagggaggc gatgccggtg cgggcccgct cgcgcgtctc gatgtccacg agaaccgcct
     6121 ggctgtcgcg tcgcagcgcc cggagcccgt ccgcttcggc gtcgaacccc tcgcgcagga
     6181 gccccgcttc gcgcaatccg atcggcggct cgtcggcgag cgcccggacg agcagatcgt
     6241 gcaggtcggg gagcgcgtcg agcccgccga gcaggcgggt gaggagggga gacgtcagcg
     6301 ccgcggcgcg cgccgcgagg ccgggcagga cggcgaggga atcacggagc gccacgagat
     6361 cgcgggcgtt cgccgtgccg agcgccgcgc gcgccaggag ccgctccaag tcgtgcaccc
     6421 ggtcgagaca ctcgcctgtc gcctggcgct ccgcggcctt ctccgacaac tccgccaccg
     6481 cctcgaggcg ggcctcgatc gcctccacct ggacgagcgg ccgcagcagc cagtcgcgca
     6541 gcatccggcc gccggcggag gtgagggtgc gatcgaccac ctccaggagc gttccgtcgc
     6601 gcccgccgtc gcgggcgttc tcgacgatct cgagggtgcg ccgggtggcg gcgtcgaggc
     6661 tcatggtgtc gccgctctcg tgccacgcga gccgcaccac gtggcgcagg tcggttttct
     6721 gcgtgtcctg caggtgggcc agaagcgccc cggccgcggc gatcgcgagg tctttgcctt
     6781 cgcagccgaa cccctcgagc gacgccgtgc cgaaccgctt gaggagcgtg gagcgcgcac
     6841 cgtcccgcgc gaagcgccag gaagggacca cggtcagccg cggcggtccg tcccggtccc
     6901 cccagagcca ggcgggggtc tcctccccgt cgcgcacgac gatctcgcgc ggcgcgaagg
     6961 cggcgacgtg ctccaccagc gacgtccgcg cgtcgcggcc gcggtgctcc gagatgcgga
     7021 actcgccggt cgagagatcg aggagcgcgc atccgaactc gtcgtcggga ccgaacgcca
     7081 cggcggcgat gaaggagggc tcgcgggctg gcaggtgggc cggatcggtg agggtgccgg
     7141 gggagacgac ccgcgtcacc tcgcgccgca cgatcccttt cgcctgcttc gggtcctcga
     7201 cctggtcgca gatggcaacc cggtgcccct cgttgacgag acgcgcgatg tagccgtcgg
     7261 cggcgtgata cgggacgccg cacatcggag cctctgaggc ggtgccgcgg ccgcgggcgg
     7321 tgagggtcag cgccagggcc ttgctcgcga ccagcgcgtc gtcgaagaac atctcgtaga
     7381 agtcgccgag acggaacatc aggatcgcgt ccggatgctc cgccttcacc tgccggtact
     7441 ggcggagcat cggcgtcagc gcgtccatgc gtctccttga caacccgaga gccgcccatt
     7501 ataaacgccg ggccacgccg cttcgcggcg cgatcgccac cgaaccatgg atcccgagcc
     7561 gctccccgtc gaagaaagcc ccgtcgcccc ggacgatctg gacgaagagg acaaggtgat
     7621 gctcgtcttc tcgtacctgt gggtgctggc gctggtgccg ttcatggtgt cgcgtcgcga
     7681 gtacgtccgc tggcacgcgc gccaggggct cctgctgacg ggtgtcgccg gcgtcgtctt
     7741 cttcggcatc atcgtggtcg gcgcgatcct gtcgaccgtc acgaaggtgt tcgcctgggt
     7801 gttcgggttc gcgctcctga acctgatctt cctctacatg gcgctggcgc tcgtctgcat
     7861 gatcaaggcg atgcgcgggg agcgctgggc gatcccgttc ctcggcgatc tcgtcgagcg
     7921 gttctagacc tcttttgctc gattcgatcc accagaccct cctcgcgaat ccgtgggccg
     7981 ggctgctgct cgtcgccgcg ctcgcctggc tcgagtacgt cttccctccg tcgccgggag
     8041 acagcgccat gctgctcgcc tgttttctcg cgggggcggg ctcgctgccg gcggtgggaa
     8101 cctacgccgc gtgtcttctc gggagcgtcg ccggggcgct gaccgcgtac gcgctcgggg
     8161 cgcggctcgg gcgctcgtac ttcttcctcc gatcggagtg ggcggccggc gagctgcagc
     8221 gcttcgagcg gggactggcg cgcttcggcc cgcggctcct cctcgtcaac cgtttcctgc
     8281 cgggggtgcg cggcgtgttc ctgtacggcg ccggcatcgg gaagcttccc ttccggccag
     8341 tgctggtcta ttccagcgtc ggcaacctcc tgtgggtcgc gctgatcgcc tgggccggga
     8401 cgagcctggg ggactcgtgg gaggaggtgc aggggatctt ccggcgctac gtctggggga
     8461 tcgggatcgc gctcgggatc tacgtgacgg tggggttcgt gcgggcgcgc cggcgggcgc
     8521 gtctcaggac ttcttcctga gccccgcttc gatccggcgc agggcggcca cccgatccgg
     8581 ctgatcgggc cgatccttga gcgaccgccg cagcgcctcg gcggcgcgcg gcatgtcccc
     8641 cttctgctcg tacgcctgcg ccatcgagtt cagcagggcg acgctcgggg gggccgcggc
     8701 ggccgctctt tcgaagaagc cgatcgcctc gtcgatccgg ttctgcatcg cggcgaccac
     8761 gccgaggttg tacagggcga gggcatggtc cggcttcagc ttcagcgcct cctcgtaggc
     8821 ggcgcgggcg tcgtcgtagc gcttctgctg cacccgcagc gtgccgaggc tgacccgaaa
     8881 ctccggcgtc ttcggcgcca ggttgatggc ggtcgtgaat gccaactccg ccttgtcggc
     8941 ctgccccatg cgcatggcga cctggccgag ccagtcgtgg tgcacggggg agtccttctt
     9001 cttctgcagg ctcgcgatca gcagccgctc caggcggccg accgtctgcg ggtccccctt
     9061 ccgcatggac aggacgaaga gccggctcat cggctcgagc gccgtcggat cgaggtagag
     9121 ggcctcgagg tactgcccct ccgcgccgcc gggattcccc tccgcctcga gcaccgagcc
     9181 catcgcggcg tggaagagcg cctggtgcgg atcctccttg agcgccgcct ccgccagctt
     9241 gcgggcggtc gccgggtact ggcggcggag gttgtccatg accacgttca gcagcacctg
     9301 ggggcccggc cggccgatgg tcagcccttc gtcggagatg ccgcgggaga tgaggtccga
     9361 cgacacttgc tgtcccagcg ccgccacgga ggccggcagg aagaggtcgt cggaggtccg
     9421 ggcgcgcgcg gccgagagga tggcgccgcg caccgtctcc tcgagatagg tggcggtgtc
     9481 ccgcccgctg cggccggcgt ggaaggtcat gacgtcgccg ggggagacgc gcgccacgat
     9541 ccggcacggc agcttgaagg tcgcctggtc gcgggtgacg atcggcagcg cctcgtcgag
     9601 cgtgaacggg gttgtgtcgt agcgatagac ccggtgatag agtggcacgc ggaggtgcgg
     9661 cccgggaggc agcaccaccg tcttcccgcc gagcgactcg cgcaccgccg cggtcccggc
     9721 tccgacccag gtgatgcagg acagacccgc caccaccagg atgacgacga tcagaagact
     9781 tgagcggcgc ataacggcgc gtgagtttat gcgagcgatc cgggcctgtc aacgaatcgg
     9841 agccctgctg gccgccgtcg ccgtggcggc tctcgccggg cccggctggg ccgcggaggc
     9901 gcgggaggag gccccgaagc ccgtcccctc ggcgacggcg gccgcccggc gcctcgaggc
     9961 gcggctcgcc ggggtcaagg cggtcgaggc cgacttcacc cagacgctcg acacgccggc
    10021 cctcaacgaa ccccaggtcg agaccgggcg gctcttcctc gagaggccgg ggaaaatgcg
    10081 ctgggactac acgcgccccg ccggcaagct ggccctcgcc gacggccggg acacgtggct
    10141 ctggcttccc gaggatcggg tcgcgatcac ggtgccgctc gaaggggacg agcgcgactc
    10201 cggcatttcg ctcctcatgc acgaccgacc cgacctgctc ggccgcttca acgtcgactg
    10261 gggcccggcg ttcaaagggg agcgcccgct ccgcctccgc ccacgccgcg ccggcgggcc
    10321 ctacgacgcg ctgctggtca ccctcgatga gtccggcttc ccgcgcgaga tcgtggtcat
    10381 cgatccgctc ggcggccgcc tgacctaccg tctctcgtca ccgcgtttcg tcgatcgcct
    10441 cgacccgacg ctcttcacct tcaccccccc gcccggcgcc accgtccagc gcgccacgcc
    10501 ctaggagcct gtccgagtcc tggacaggct cctggcgacg gtgcctccga acctttacat
    10561 ccccgagacc ggaatgctag gatgtgcgcc cttgaccgcc gtcgcggagg cgtggtggcg
    10621 tccgaaaact ccttcgacat cgtctccaag gccgacctcc aggagatcga caacgccgtc
    10681 aatcagaccc tccgcgagat cggccagcgc tgggatttca agaacaccaa agtgacgctc
    10741 acgcgcgaag cgcaggtcct gacgctgcac gccgaggacg agtcgcggcg gcgcgcggtc
    10801 atcgagatcc tcagcgagcg cctcgcctcg cggaaggtgc cgatcaaggc gctgcagttc
    10861 aaggacgcgc agcccgccgc cgtcggttcg gtgcggcagg agatccacgt gcaggccggc
    10921 atcgcccagg accaggcgaa ggagatcgtc aaggtcctca aggcgagcgg cctcaaggtg
    10981 cagccggcga tccaggggga ctcggtccgg gtgcgcggca agaacaagga cgacctccag
    11041 gcggcgatgc gggtcctgcg caccgggacc ttcgaattcg acatgcagtt caccaactac
    11101 cgctgagacg gatgagccga cgcgccggga tcgggatcgc actcgtcgcg ctgctggcgg
    11161 catctccgtc cgccggcgcc gcctcgcccg acccggcggt cgtggtgaag ggatcgctga
    11221 ccgaggcgaa cggcgccccc gcccccttcc agacggtgcg cctcatcaag acccgcaaga
    11281 tccgcagcgt ctccgatccg aaggcgcgcg cgcaggaggt cgaggaggcg cgcacccgca
    11341 ccgacgcgtc gggcgcgttc cgcttcgaga tcccggccga cccgtccttc ccgttctggt
    11401 tcgtgcgctt ctacgatccg aacgagttcg acgcggtgaa gtacaagatc ccgtccgaca
    11461 ccgacgtcag ccgcgcggtg catgacggct tcggcatcga ggtctccgcc gtcctgcact
    11521 tccatcccga ctggccgaag gtgaaggcgc tcgtggatca gtacgggccc ggaagccacc
    11581 ggggccagat cctccgcagc ctcgggctcc cgacgcgcca ggagccgcgc gacgagggga
    11641 aggagctgtg ggtgtacgcc gcctccggcg tctcgtacct tttcgacggc gacaaggtgc
    11701 tcgagacccg ccgcggcaat gcctcgtccg agtcggcgca gccggccgaa aaggtcgatc
    11761 cgcagtgacc gatctcgcga ccgggctgcg caccccgtcg gccgccgacg gcccgtcgga
    11821 ggcgccgcgc tatccgcccg agctgccggc gatcatcgcg ctcgtcgccg acaagctgct
    11881 ccgggtcgag gaggagtgcc ggcgcaatct ccggtcggag gtcggcgcga tcgacgagct
    11941 ggggcgctat ctcgcggatg gcggcggcaa gcgcatccgt ccgatcctgc tcctcctcgc
    12001 gacccagatg tgcggctaca agggggaccg ggacgtgctg ttcgcctcgg tcttcgagtt
    12061 catccacacc gcgacgctcg tgcacgacga cgtcatcgac gaggcggaga cgcgccgcgg
    12121 ccgcggcacg atgaacgcgc gctgggggaa cgggctgacg gtcctcctgg gcgactacct
    12181 ctacatcaag tcgatgaaca tggcgctcga ggcggacgac atccgcatca tcaggatcct
    12241 cgcgcagatc accctgaaga tgatcgaggg ggagatcgtc gccgaccgca agcgcgggca
    12301 gatcggcatg accgaggagg aacacctcga ggtcatccgc cgcaagaccg cgtacctgtt
    12361 cgccggctgc ggccgcgtcg ccggggtgct gtcggggctc gagccgcgcc gcctcgagtc
    12421 gatcgccgac tacggcatga acctcggcat ggcgttccag atcgccgacg acctgctgga
    12481 cgtcaccgcc gacagccgga cgctcgggaa accggccgcc tccgatctga aggaggggaa
    12541 gctgacgctg ccgctgatct acctgctcga gaccgcgtcg gggtcggagc gggagatggt
    12601 ccggacagtc ctcgaggaag gggagttcct gaccgtgtcg cgcgacgaga tcctcgcgct
    12661 cctgcggcgg catcgcaccc tcgaacgaac ccgggagctg gcggctggct atgccctgaa
    12721 ggcccgccag gcgctcgacc tcttcccccc gagccccgcc cgggacgccc tcctcttcct
    12781 gccccagttc gtcgtgtccc ggcagtacta ggcttccggg gccggatccg ggccccctac
    12841 gaattggggc tggccggacg acgatcccca agatttcgtg ttttcgaggg tccggacggg
    12901 cgcgatcccc gtgctatatt cgcggcctcg gcgcggacgg ctctcccgcg ccctccgggg
    12961 aggtcatggc gcgattcctc aagctcgaac ggatggagat ccacggcttc aagtcgttct
    13021 atggccgcac ccgcttcgac ttcccggagg ggatcaccgc ggtcgtcggt ccgaacggct
    13081 gcggcaagag caacatcggc gacgccatct cctgggtgct cggcgagcag cgggcgtcct
    13141 cgctgcgtag cgagagcatg gaggacgtga tcttcaatgg cagccagggg aagaagcccc
    13201 tggggatggc cgaagtctcc ctccacttca agaacatccg cgcgcgggtc ggggacaccg
    13261 cctgcggcac gcttgacggc gagccggggg tcgcaccgga agcgatgatg gatccgctcg
    13321 gcgacgcggc cgccgccctc gccacgccgg gcaacgggaa cggccagggc aacggcaacg
    13381 gtcacggtca cgggaacggc cacgccgtcc acgccgccgt cgaagcggag cctggggccc
    13441 cgatcgaagt gctctccgcc gacgtcgccg tcagcgaagt cgcgccggac cccgggacgg
    13501 aggcggcgga ggccggggcg ccgaccgagg gggacaccgg gacgcaggcg gcgcgcttct
    13561 tcctcgagga gcttcccgac gaagtggtcg tgacgcggcg gctgtaccgc tccggcgaga
    13621 gcgagtacgc gctgaacggc cagcgctgcc gcctgcgcga cgtgcaggac ctcctgtcgc
    13681 gcacggagat cggcacccgc ctctacacca ccatcgagca ggggaagatc gatcagattc
    13741 tcctcgcgaa gccgaaggag cgccgcgtca tcatcgagga ggcggccggc atcctgggct
    13801 acaagacgaa gcggcgccac accgagcaga agctcgaagc gacgcaggcc aacctcctgc
    13861 gtctcgccga catcgcgctc gaggtggaca agcagatcca gtcgctccga cgccaggcgg
    13921 cgaaagcgcg ccgctaccgc cgtctcatgg acgccctgcg cgatcgccgc gcctcgctcg
    13981 cgtggcggaa gctcatcgac tgcgacgccg gcctcgaggc ctcggcgcgc gccctggggg
    14041 agctcgggga gcgcgaagcg gccgcggccg gcgagctcgg gcgggcggag gcggatctcg
    14101 aggcgctgcg cctgaagctg gacgccgcgg aggaggaggc gcgctgccgg cgcgacgcgc
    14161 tgcacgcgct cgacctcgag ctcgatcgca tccaggagcg gcagcgggcc ggggccgagc
    14221 aggccgtcga tctcgagcgc cgcgcttccg aggcacagga ggagatcggc acgctgcagg
    14281 cgcgcgctga cgagcgcgag accgccgcac gcgccctgac cgaggacctg gagcgggagg
    14341 cggagcgcgt gcgcgccgcc gaagcggggc tcgccggtct ggaagccgac cggtcggcgc
    14401 gggccgccgc catcgcgcgc tgcgaggagg agctggaggg actgcggcgg cggttcctgg
    14461 atcgcctcga tcgcctcgcc gaaacgggac ggcggcgcgc cgcgctcgag gagcaggggc
    14521 gcgcggcgct cgtggcgctc gagcgcctgg cgcgcgatcg cgccgacgcg gagcgggccg
    14581 gcgaggagat cgaacgaacg ctcgtcctcc tcgaggcgga gacgaccgcg cggcaggggc
    14641 gcgtgcagga gtgccagcgt ctccaccagg acgaggggac ggcgcggcgc ggcgcggagg
    14701 aatcgctcgc tgaagccgag ctgcggctcg aggagcggcg cggccgttac gccgccctgt
    14761 cggagcggct cgaggccctc gccgacctcg agcgacgcca cgccggcatg gccgaggggg
    14821 tggtcgatct gctgcgcgga ggaggcgggt tcgagccgcg cggagtcgtg ggggagacgc
    14881 tcgacgtccc gcccgggctg gagcgggcgg tcgccgccgc cctcggtccc cttcaggacg
    14941 gcctcgcggt cggcgcggtc gaggaggcgg cgcgtggcgc gcggctcctg cgccagcgcg
    15001 gcaccgggcg cgcggcgttc gttccggcgg gcgccgggcg cgtcgccgcg gcatcgcgcc
    15061 cccccgaagg gatcccgggc ctgctcggag tcctcgccga acgggtcggc gggttggagc
    15121 cggggggggt cctcgaggcg cgcctgtcgc gcatcctgct catgagcgac ctcgacgccg
    15181 ccgtcgccgc ggccgcgcac cttcccggct ggagcctggt gacgccggag ggcgacctcc
    15241 tcgactcgga cggcgcgctc tacggcggcg acgggccgga gcagcagcac ggcgttctgg
    15301 cgcgacgggc cgagcgggcg gggctcgcga agcagatcga ggagatcgac gccgagcgtg
    15361 cggaggcgga gcgggactgc gaggcgctca agcgcgggac cgaggaacgg cgcgcccggg
    15421 ccgcggcggc ggacgctgcg ctccaggagg agcagcgcgg gctcttccag tcggaccatg
    15481 cccggcagca gaaggatgcg gagcgcggcc gcctgctcgg cacgcttccg ctgctcgaaa
    15541 gcgaagcggg acggctggag cgtgaggcgg cggcgcgcgc cgaggaggcc gcccgggcgg
    15601 tggcggagca ccaggagcac gagaccgagc gcctcgcggc cgaggaggcc atccgcggcg
    15661 cgacggaggg gatcgccctc atgcgcggcg gtctcgacga ggcgcaggga cgcgcctccg
    15721 aggcgcgcgc cgcggtcgcg gccgggcacc agcggctggc cgccctggcg cgcgagcgcg
    15781 agtcggtgcg cgaggcggag cgggagctgc ggacacggct cgccgaacgg gggcagcagc
    15841 gccaggcggc gcacgagcgg ctggcggaga tcgcccgtca ggcgtccgat ctcaccgggc
    15901 agcgcgagcg caccgccgcg acccgcgcgg aatcggcggc ccaggacgaa gcggccctcg
    15961 ccggcctcgc ctacgatcgg tcgctcttcc acgcgcgcga gcagacggcg aaggagtcgc
    16021 gcactctgct cgacgcggcc cgctccgagc gccaggagca ggagatccgg cgggcccggc
    16081 tgtcgagcga catcgagcac ctcgaggcct cctgccgcga ggacctcggc ctcactctcg
    16141 aggagctgag ggcgaatcct ccggccctcg aggaagggcg gaccctcgcc gacgacgaga
    16201 aagacgtcgc cgagggtcgg gcgtcgatcg attccatcgg cccggtgaac ctcatggcga
    16261 tcgagcagtg caccgagctc gaggagcgcc acggtctcat ggagaaggaa cgccaggacc
    16321 tcgaggcggc gatcgagtcg ctgcgcgaca ccatccggcg catcaaccgc gagtcgcggc
    16381 agcggttcct ggtggcgttc gaggcgatca aggcggggtt cgaagcatcg ttcacggtcc
    16441 tcttcggcgg cggccgggcc gagctgcggc tccaggaggg ggaggaggac gtgctcgagg
    16501 ccggcatcga gatcaacgcg cag
//
LOCUS       DASRIQ010000504         5684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_211032, whole genome shotgun sequence.
ACCESSION   DASRIQ010000504 DASRIQ010000000
VERSION     DASRIQ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211032"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFD06_12285"
     CDS             complement(<1..578)
                     /locus_tag="VFD06_12285"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HZN04351.1"
                     /translation="MAKHQEQVPALDPEVTSSGTGGAEEAEAQRAAARLGIPYVDMAE
                     FKINQDLFRSIPVDLMFRYNFIPWKEEEGRLVVVMSDPSDVLMVDELETLVGKPLQVA
                     VGTRQAIADVLKKSESGQRVLEAATEEFRIQVVREDDETGEETLTIDKLTSDQSPIIK
                     LVDSTLFNALQRRASDIHVETRDREVVIKYRI"
     gene            complement(589..1800)
                     /locus_tag="VFD06_12290"
     CDS             complement(589..1800)
                     /locus_tag="VFD06_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN04352.1"
                     /translation="MPEFVARLGTADGNILERSFTSESEKALRAELAGQYLILGVRKR
                     QTVATLLPGMGTSRGIKMKEFLLFNQELASLLKAGLPIVASLDILTERRKNPVFKQAL
                     LEVRDQVRGGMSLSEAFSAQGDLFPRIFCSTLASGERSGEIASVLTRFIAYQKTMLST
                     RRKVAGALIYPAFLLLVSVGVITILVAFVVPKFEDFYADMGADLPLITRVLLGLSKVM
                     TSYAPLVLVAVVGGLIGWRAWSRTAGGRLAIDRMKLRIPVIGGLLQGFAASRFVRTLG
                     TLVAGGIPVVAALAIAARAVGNLVFERALLEVERKVREGGGLWQALEETGLFSDIAIE
                     MTKVGESTGALSEMLGNVSDFYDEEIDNRLATLMALMEPVMLIGMGIIVALMLLAMYL
                     PLLQSYSLTGG"
     gene            complement(1815..2570)
                     /locus_tag="VFD06_12295"
     CDS             complement(1815..2570)
                     /locus_tag="VFD06_12295"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZN04353.1"
                     /translation="MSPRIGIPATLVLLALSLPFLPVVPTSAAPGAGVSVVPAPPGPD
                     GLVTRTFTLRYKAVDDAYLLLSPWLSPKGSIRTQPHQKSITVTDTPDAVRQATTLLSG
                     FDIPPRVVQVAVQLILASSEKNPIVPTPPPIKGVVDRLNALSTRWSDYKLVGDARILG
                     TEGERGTLKVGDDYRVDFRIDQIADESRIIRFKPFELARRELQVEGQERYQPILNTVL
                     NLRDSQLFIVGASKMERSDRALFMTITASLSIP"
     gene            complement(2580..3272)
                     /locus_tag="VFD06_12300"
     CDS             complement(2580..3272)
                     /locus_tag="VFD06_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN04354.1"
                     /translation="MDHGSGTTRVDCTSIAPLLGHYLAEQVDEATRMKVRTHLLTCPS
                     CSTEALKLDPSILFVGLGQERPSPESWARFDVALRSRIEAEAGKRRGIFSGWDFSRLE
                     TAVRMPRLAFATPLAMLAVLAGLVFVSQPGMILRGPRPRIEAIRPPHEIGGPVRPAPD
                     PRGPAGGFDTGRHASLRLAGAEAALLPTLEEVSSPAARVYRLDVGASAAAATDGTDSS
                     AVYFVVDETINF"
     gene            complement(3411..3989)
                     /locus_tag="VFD06_12305"
     CDS             complement(3411..3989)
                     /locus_tag="VFD06_12305"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN04355.1"
                     /translation="MNDQDLVERAAAGDLDAFTELVESRRDRVFRIARHLVGDEELAR
                     DIAQDVFFRLFRVIHRFRKGGRFDAWLHRMTVHLGIDALRRERPHRQTASLEEISGRQ
                     DTVPSPQPGPAQALGAQEVRRLFADLSRRLGRRQRAAFILREIEGLSTLEVAEVLGTS
                     ESTVRNHILQARKVLQKALRERFPEYLPPERR"
     gene            complement(3986..4984)
                     /gene="thiL"
                     /locus_tag="VFD06_12310"
     CDS             complement(3986..4984)
                     /gene="thiL"
                     /locus_tag="VFD06_12310"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZN04356.1"
                     /translation="MPFHLSEDSFIALMRRLSRGAGPKPVAGIGDDAAVLQPPRRSHT
                     LVTTDLLTEGVHFLRARTPARLLGRKAMAVNLSDIAAMGGVPLQAVVSVGLPRGTKAS
                     FARDLARGLAEQARRHRVAIVGGDTCAAQRLFVNVTLLGAVEPGRAVRRSGARPGDRL
                     YVTGTLGASAAGLTLLRAGRTRVNADAATRHVLRVHLDPTPRVLFGRALGLGGLAMAM
                     IDLSDGLGKDLPRLCEASGTGAVISESALPVDRAALALLGEAEGRLAAIAGGEDYELL
                     FAARSGTEPILAVLAARLRQKVTAIGTLVPRRQGIRLLGRDGGYRPLPGPAFEHFR"
     gene            complement(5043..>5684)
                     /locus_tag="VFD06_12315"
     CDS             complement(5043..>5684)
                     /locus_tag="VFD06_12315"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZN04357.1"
                     /translation="GVPRVTPEMTLQRDQVGVATGLAWTAAGGEILFVEATTMPGRGR
                     LILTGHLGAVMKESAQAALSYARSRAADFGIGVNAFGEMDVHVHVPEGAIPKDGPSAG
                     ITMATAILSVLTGRPVRRDIAMTGEITLRGNVLPIGGVKEKVLAARRAEASTVILPEG
                     NRRNLEDVPKLFRKGLRFVFVSDVHQVLQEALGEPVARPRAPRVAAGRTVAAL"
BASE COUNT          855 a         1958 c         2062 g          809 t
ORIGIN      
        1 tcgatccggt acttgatcac cacctcgcga tcgcgggttt cgacgtggat gtccgaagcg
       61 cggcgctgga gggcgttgaa cagcgtcgag tcgaccagct tgatgatcgg cgactgatcg
      121 gaggtgagct tgtcgatcgt cagcgtctct tcgccggtct cgtcgtcctc gcgcacgacc
      181 tggatgcgga actcttcggt cgccgcctcc agcacgcgct gccccgactc ggacttcttg
      241 aggacgtcgg cgatcgcctg gcgcgtcccg accgcgacct gaagcggttt gccgacgagc
      301 gtctccagct cgtccaccat caggacgtcc gacgggtcgg acatcacgac gacgagccgg
      361 ccctcctcct ccttccaggg gatgaagttg tagcggaaca tcagatcgac cgggatggag
      421 cggaacaggt cctggttgat cttgaactcg gccatgtcca cgtacgggat gccgagacgg
      481 gcggcggcgc gctgcgcctc cgcctcctcg gcgccgccgg tcccggagga ggtcacctcc
      541 gggtcgagcg ccgggacctg ttcctggtgc ttggccatgt cgtgtcccct agcctccggt
      601 cagcgagtac gactgcagca gcggcaggta catcgcgagc agcatcagcg ccacgatgat
      661 ccccatgccg atgagcatca ccggctccat gagggccatc agcgtcgcca gacggttgtc
      721 gatctcctcg tcgtagaagt cggacacgtt ccccagcatc tcggagagcg cgccggtgct
      781 ttccccgacc ttggtcatct cgatggcgat gtcggagaag agcccggtct cctcgagcgc
      841 ctgccacagc ccgcccccct cgcgcacctt gcgctcgacc tcgaggagcg cccgctcgaa
      901 gaccaggttg ccgacggcgc gcgctgcgat ggcgagcgcc gcgacgaccg ggatgccgcc
      961 cgcgacgagc gttccgagcg tgcgcacgaa gcggctggcc gcgaacccct gcagcagccc
     1021 tccgatgacc gggatccgca gtttcatccg gtcgatcgcg aggcgtcctc cggcggtgcg
     1081 ggaccaggcg cgccagccga tgagcccgcc gacgacggcc accaggacca gcggcgcata
     1141 gctggtcatc accttcgaca gtccgaggag cacgcgcgtg atgagcggga gatcggcccc
     1201 catgtcggcg tagaagtcct cgaacttggg gacgacgaag gcgaccagga tcgtgatgac
     1261 gccgaccgac acgagcagca ggaacgccgg gtagatgagg gccccggcca ccttgcggcg
     1321 cgtggacagc atcgtcttct ggtaggcgat gaagcgggtc aggaccgagg cgatctcgcc
     1381 cgagcgttcc cccgaggcga gcgtcgagca aaagatgcgc gggaacagat ccccctgcgc
     1441 cgagaacgcc tcggagaggg acatcccgcc gcgcacctga tcgcgcacct ccagaagggc
     1501 ctgcttgaag accgggttct tgcgccgctc ggtgaggatg tcgaggctgg cgacgatcgg
     1561 caggccggcc ttgaggagcg acgccagctc ctggttgaac agcaggaact ccttcatctt
     1621 gatgccgcgg gacgttccca tcccggggag cagcgtggcg accgtctgcc ttttccggac
     1681 gccgaggatg aggtactgcc cggcgagctc cgcgcgcagc gccttctccg actccgaggt
     1741 gaaggagcgc tcgaggatgt tgccgtcggc ggtgccgagc cgggcgacga attcgggcat
     1801 cggtgctcgg gctcctacgg gatcgaaagc gaagcggtga tggtcatgaa cagcgcgcga
     1861 tcggagcgct ccatcttgga ggccccgacg atgaacagct gcgagtcgcg caggttgagg
     1921 acggtgttca ggatcggctg gtagcgctcc tgcccctcca cttggagctc gcggcgcgcc
     1981 agctcgaacg gcttgaagcg gatgatgcgg ctctcgtcgg cgatctggtc gatgcggaag
     2041 tcgaccctgt agtcgtcccc caccttgagc gtgccccgct ccccctcggt cccgaggatg
     2101 cgggcgtccc cgaccagctt gtagtcgctc cagcgcgtcg agagggcatt gaggcgatcg
     2161 accaccccct tgatcggcgg cggcgtcggg acgatcgggt tcttctcgga cgaggccagg
     2221 atgagctgca ccgcgacctg caccacgcgc ggcgggatgt cgaacccgct caggagcgtg
     2281 gtggcctggc ggaccgcgtc cggcgtgtcg gtgacggtga tggacttttg atgcggctgg
     2341 gtgcggatcg atcccttcgg cgacagccac gggctcagga gcaggtaggc gtcgtcgacc
     2401 gccttgtagc gcagcgtgaa ggtccgcgtc acgaggccgt ccggcccggg cggggccggc
     2461 accacgctca cgccggcacc gggcgccgcg ctcgtgggaa ccacgggcag gaacggaagg
     2521 gacagggcca gaaggacgag cgtcgccggg atcccgatgc gtgggctcac gaatgtcccc
     2581 tagaagttga tggtctcgtc gaccacgaaa tagacggcgc tggagtcggt cccgtccgtg
     2641 gccgcagcgg cggaggcgcc gacatcgaga cggtagacgc gcgcggcggg agagctcact
     2701 tcttcgagcg tcgggaggag ggcggcctcg gcgcccgcga ggcgcaggga ggcgtgccgg
     2761 ccggtgtcga atccgcccgc ggggccgcgc ggatccggag ccggccggac cgggccgccg
     2821 atttcgtggg gcgggcgaat cgcttcgatc cggggccgcg gcccgcgcag gatcatcccc
     2881 ggctggctga cgaagacgag gccggccaga acggccagca tcgcgagcgg ggtggcgaag
     2941 gccaggcggg gcatccgcac cgccgtctcg agccggctga agtcccagcc gctgaagatg
     3001 ccgcggcgct ttccggcctc ggcctcgatg cgggacctga gcgcgacgtc gaaccgcgcc
     3061 caggactccg gggacggccg ctcctggccg agacccacaa acaggatgct gggatcgagc
     3121 ttcagggctt ccgtggagca ggaggggcag gtcaggaggt gggtgcggac cttcatacgg
     3181 gtggcctcgt ccacctgttc ggcgaggtag tgtccgagga gcggggcgat cgaggtgcag
     3241 tcgacccggg tcgtaccaga accgtgatcc atgccggtct cccttgggtg accggcggat
     3301 tcggcgttcc cggtcgctgt ctatacgggc cggcgcggga atccgtctat ggtgctttga
     3361 cgcatgcgcg gtggcggaaa tcgaccccgc cacccctgga ctccccggga tcagcggcgt
     3421 tccggaggga gatactccgg aaaccgctcc cggagagcct tttggaggac cttgcgggcc
     3481 tgcaggatgt ggttacggac ggtcgattcg ctggtcccca ggacctccgc cacctccagg
     3541 gtggagagcc cctcgatctc gcgcaggatg aaggcggccc gctgtcggcg tcccagccgc
     3601 cgggagaggt cggcgaacag ccgccggacc tcctgcgccc ccagcgcctg cgcggggccc
     3661 ggctggggcg acgggaccgt gtcctgccgc cccgagatct cctccaggga ggcggtctgg
     3721 cggtgcgggc gctcgcgccg cagcgcgtcg atgccgagat ggaccgtcat ccggtgcagc
     3781 caggcgtcga accgcccccc cttgcggaac cggtggatga cgcggaacag gcggaagaag
     3841 acgtcctggg cgatgtcgcg cgccagctcc tcgtccccca ccaggtgccg ggcgatccgg
     3901 aagacgcgat cgcgccggct ctccacgagc tccgtgaagg cgtcgaggtc gcccgcggcg
     3961 gcgcgctcga cgaggtcctg atcgttcacc ggaagtgctc gaaggcgggg ccggggagcg
     4021 gccggtagcc gccgtcgcgg ccgaggaggc gaattccctg cctccgcggc acgagggtgc
     4081 cgatcgccgt caccttctgg cgcagccgtg cggcgagcac ggcgaggatc ggctcggtcc
     4141 cggagcgcgc cgcgaacaga agctcgtagt cttccccccc ggcgatcgcc gcgaggcgcc
     4201 cttcggcttc gccgagaagc gccagggccg cccggtccac gggaagcgcg ctctccgaga
     4261 tcaccgcccc cgtgccggag gcctcgcaca gcctcgggag gtccttcccc aggccgtccg
     4321 acagatcgat catcgccatg gcgagcccgc cgagcccgag ggcgcggccg aacaggacgc
     4381 ggggcgtcgg gtccagatgg acgcgcagga catgccgggt ggcggcatcg gcgttcactc
     4441 gcgtgcggcc cgcgcgcagg agggtcagtc cggccgccga ggcgccgagg gtgccggtca
     4501 cgtaaagccg gtccccgggc cgggcgccgg atcggcggac ggcgcggcct ggctcgacgg
     4561 cgccgagaag cgtcacgttc acgaagaggc gctgtgcggc gcaggtgtcg ccgccgacga
     4621 tcgccacgcg gtgccggcgg gcctgctcgg cgaggccccg cgccaggtcg cgcgcgaagg
     4681 aagccttcgt tccccgcggc aggccgacgg agaccacggc ctggagcggc accccgccca
     4741 tcgccgcgat gtcgctcagg ttcaccgcca tcgccttgcg gcccagaagg cgcgccggag
     4801 tccgggcccg gaggaagtgc accccctcgg tcagcagatc ggtcgtgacc agcgtgtggc
     4861 tccgccgcgg cggctgcagc accgccgcat cgtcgccgat gccggcgaca ggcttcgggc
     4921 cggcaccgcg gctcaggcgg cgcatcagcg cgatgaacga gtcttcgctg agatggaacg
     4981 gcatcggcgt cgcgtcgcga ttccccccga cggcgggtgg gaggccggcg ggcgccggga
     5041 ttctacagcg ccgcgaccgt gcggccggcc gccaccctcg gggcgcgcgg gcgggcgacc
     5101 ggctcgccga gcgcctcctg cagcacctgg tgaacgtcgg agacgaagac gaagcgcaac
     5161 cccttgcgga acagcttcgg cacgtcctcg aggttgcgac ggttcccctc cggcaggatg
     5221 acggtcgagg cctcggcgcg ccgggcggcg agcaccttct ccttcacgcc gccgatcggc
     5281 aggacgttgc cgcgcagggt gatctcaccg gtcatcgcga tgtcgcgccg caccgggcgg
     5341 ccggtcagga ccgacaggat cgccgtcgcc atggtgatgc cggccgacgg cccatccttc
     5401 gggatcgccc cctccggcac gtggacgtgc acgtccatct cgccgaacgc gttgacgccg
     5461 atgccgaagt cggcggcgcg cgagcgggcg taggacaggg ccgcctgcgc cgactccttc
     5521 atcaccgccc cgaggtggcc ggtcaggatg agacggccgc gccccggcat ggtggtcgcc
     5581 tcgacgaaga ggatctcccc tcccgccgcg gtccaggcga gcccggtcgc aaccccgacc
     5641 tggtcgcgct gcagcgtcat ctcgggggtg acgcgcggca cgcc
//
LOCUS       DASRIQ010000505         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2529666, whole genome shotgun sequence.
ACCESSION   DASRIQ010000505 DASRIQ010000000
VERSION     DASRIQ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529666"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VFD06_12320"
     CDS             complement(<1..1277)
                     /locus_tag="VFD06_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HZN04358.1"
                     /translation="MSPISAPRVNPGPDYRLGPGDLLEIQITGRVDVTRQQITVDLDG
                     RVNVPPIGAVDVGGLPLLEAHRRIVERARALLRYADISVTVLAPRTFEVVLSGEVQRP
                     GAILASATRRVHELIQSAGGITPAGSWRRVTVARKGVQRQIDLLEFELRGDVAQNPAA
                     EEEMTIYVPPRAGSVTLAGAVRRPGEYEVHPSGSLAELLELTGGLVQGAAPGEARLTR
                     VGPEGRKETISVDLAAALSTSTDLTLRPGDLLFVPSLTVLQDVVELRGAFNGTAESSK
                     TTTAGKPTIVQRFELARGERVRDFVFKAGGAAAFADLRQAFVERAGTAGPRQRIPIDL
                     HQLLVEKDETQNVVLDNGDVFTLPVIEDKVYVVGEVKTPGAVDFRPDFTAREYVTVAG
                     GPGVRAKVSNTTVTFRNGRTYAMKDAPPLEPGAV"
     gene            1760..>2030
                     /locus_tag="VFD06_12325"
     CDS             1760..>2030
                     /locus_tag="VFD06_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN04359.1"
                     /translation="MRPAVVNIGTVQVSHSGRPPVIPGPFSDDPFFKDFFDQFFGRRG
                     SGRQEEFRQPGLGSGVIIDKRGYVLTNYHVVRGADAVTVRLSSKQE"
BASE COUNT          310 a          690 c          690 g          340 t
ORIGIN      
        1 accgcgcccg gctccagcgg cggcgcatcc ttcatcgcgt acgtgcggcc gttgcgaaag
       61 gtcacggtgg tgttgctgac cttggcgcgc acgccggggc cgcccgccac cgtcacgtac
      121 tcacgggccg tgaagtccgg acgaaaatcc acggcccccg gcgtcttcac ttcgccgacc
      181 acgtacacct tgtcctcgat gacggggagc gtgaagacgt cgccgttgtc gagcacgacg
      241 ttctgggtct cgtccttctc cacgaggagc tgatggaggt cgatcgggat acgctggcgc
      301 ggccccgccg tgcccgcgcg ctccacgaac gcctgccgga ggtcggcgaa ggcggcggcg
      361 ccacccgctt tgaagacgaa gtcgcgcacg cgctctcccc gcgccagctc gaagcgctgc
      421 acgatcgtcg gcttcccggc cgtggtcgtc ttgctcgact ccgccgttcc gttgaaggcg
      481 ccccgcaact ccacgacgtc ctgcagcacg gtcagcgacg gcacgaagag caggtcgccg
      541 gggcggagcg tgaggtcggt ggacgtgctg agcgcagccg cgagatcgac ggagatcgtc
      601 tccttgcgtc cctccggtcc cacgcgcgtc agccgcgcct cacccggcgc cgctccttgc
      661 acgaggccgc ccgtgagctc gagcagctcg gcgagcgatc cactcgggtg aacctcgtac
      721 tcgccggggc gtcgcaccgc gcccgcaagc gtgaccgagc ccgcccgcgg gggaacgtag
      781 atcgtcatct cttcctcggc ggcgggattc tgcgcgacat cgccacgaag ctcgaactcg
      841 aggagatcga tctggcgttg aacccctttt cgcgctaccg tgacgcggcg ccagctgccg
      901 gcaggcgtga tgccgcccgc ggactggatc agctcgtgga cacgccgggt cgccgaggca
      961 agaatggcgc ccggccgctg aacctcgccc gagagcacga cctcgaacgt gcgcggagcg
     1021 agcacggtga cggagatgtc cgcgtagcgg aggagtgcgc gggcgcgctc gacgatccgt
     1081 cgatgcgcct cgagaagcgg gagcccgccg acgtcgaccg cgccgatggg cggaacgttg
     1141 acgcggccgt cgagatcgac cgtgatctgc tggcgcgtga cgtcaacgcg accggtgatc
     1201 tggatttcga gcagatcgcc gggtccgagc cgataatcgg gccccggatt gactcggggc
     1261 gccgagatcg gcgacatgac gtcgggcttc gtggtcgttg ccggaaccga cgacccggcc
     1321 ggcggtgtcg gagaaggcga cgattgctgg gcgaccgaga gggatggcga tagcaaggac
     1381 accgtcagaa cgacacagac ggctttgcgc gcagtgagag acggtaactt aacggacggg
     1441 gaaacttttt gcaatatggc attttctctt tttcagcgcc gtagggtcaa ggaaaaaggc
     1501 gtacgtttgc aatagtttcc tcgacattta gccctcctga cgcgggtgat tcagccgccg
     1561 gctccggcgc ttccggggac cgccgatgac cccccggacg gcccggacgg ggctcctcgt
     1621 catcgctctt ttggcctttg cggctgctgg gttcggcgtg cgggcggtgt ggccgtcccg
     1681 gtccatccct cccccgcccc cctcgcccct gggtaccgcc gacgccctcc agtcggcctt
     1741 cgtaggtgtc gccgaacggg tgcggccggc cgtggtcaac atcgggaccg tgcaggtgtc
     1801 tcattcggga cgcccgccgg tcatcccggg gccgttctcg gatgacccgt tcttcaagga
     1861 cttcttcgat cagttctttg gacggcgggg ttcaggccga caggaggagt tccgccagcc
     1921 cgggctcggt tcgggcgtca tcatcgacaa gcgcggctac gtcctcacca actaccacgt
     1981 cgtgcggggt gccgacgccg tgaccgtccg gctttcctcg aagcaggaat
//
LOCUS       DASRIQ010000506         3283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_731434, whole genome shotgun sequence.
ACCESSION   DASRIQ010000506 DASRIQ010000000
VERSION     DASRIQ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731434"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VFD06_12330"
     CDS             complement(<1..1283)
                     /locus_tag="VFD06_12330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HZN04360.1"
                     /translation="MLEQAAGDKPQVAAESAPTFRIDVPLYTLLQKTSARSALAPLAE
                     PVTLPEPIFPQIRRTKDLKQRGLFYNANNGVRAFRRWLIPYIQSRVMPNEFRPLLSYL
                     FTEWKCNLDCHYCWAFDNSVKGMTEEVAKRSIDFLHSVGCRVTAIMGGEPLLRPKFIH
                     KVIYYGSKKGFFMYLPTNGRLMKPQVVDWIGDAGVAAINLAVDCVEERPGLPKALDRI
                     RPYFDYLVKMQRRYGYIVVFNMNICRNNMADIRELTEIAHDHGISTDYHINETPMIEQ
                     GHFKHYEENATYLRPEDYPLVDELLDHLTEKNRQGYIMVNSKAHLQKMKAFMRGHVDP
                     WQCRAGQNSMLIRTDGTVAPCFPMYSATYDWGTIENQKFDFRQLDTMREGCNTHCLST
                     TQYVLGHYYNNQTVLRWIVKQALHGMSGGSTAVAQA"
     gene            complement(1391..>3283)
                     /locus_tag="VFD06_12335"
     CDS             complement(1391..>3283)
                     /locus_tag="VFD06_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN04361.1"
                     /translation="PLGWPRTTDSLCPKCVPEIRQQILDGELPVEILRRERVGEIKAQ
                     VIERDGKILMVKDCPVHGHFEDVMSVDPEMFRHLEESFPGRDIRAHSDKKLHDHGTST
                     IQHGRGAVLTIDLTNRCNMMCDPCFMDANQVGFVHELSWEEIQTLLDNAISIKPRRQL
                     SVQFSGGEPTLSPHFIQAIEYSRKVGYTSVQAATNGIEFAKSKDFCRRAAEAGLRYAY
                     LQFDGIGNAANSHRKVGNLFDVKMRAIENLVEAGVDIVPVTTIVNGINNEQVGRIIKF
                     ALDNPKIVSLCSFQPVSFTGRDEAITDERRQAQRYTLSHLAHDVRQQTGLGDPRRDWF
                     PISFLGTFSDWADLVHGPDREWGQLNCGCHPNCGVGMSVMVDKETKEAATLTSFLDVY
                     QLARDVAKINDAGRGRFLSVLGMGLAVVKNFDPFKATTHFHLWDMLMKFDKGFGASGR
                     DYGKVGADRTREDIERRRRDRWNVFFIAGMWFQDLFNYDFRRTEMCIIPYATQEGEIS
                     FCAYNTGVGWRNIVEKMHMTATLTKWYEEHGRHEIFAGGKSVGLDSLAHSMVLREEIV
                     TLEEQHDLDELGIAKNAREEKVRARAERLRREREHERLAQLYRQHVLKEAEAPALVQI
                     QGLGRE"
BASE COUNT          514 a         1069 c         1056 g          644 t
ORIGIN      
        1 gcctgcgcca ccgccgtcga gccgccgctc atgccgtgaa gcgcctgctt gacgatccac
       61 cgcagcacgg tctggttatt gtagtagtgc ccgagcacat actgggtggt tgagaggcag
      121 tgcgtgttgc agccctcgcg catcgtgtcg agctgcctga agtcgaactt ttggttctcg
      181 atcgtccccc agtcataggt cgccgagtac atcgggaagc agggcgccac cgtcccatcg
      241 gtccggatga gcatcgagtt ctgccccgcc cggcactgcc acgggtcgac atgcccgcgc
      301 atgaaggcct tcatcttctg gaggtgcgcc ttcgagttga ccatgatata accctgccgg
      361 ttcttctcgg tcaggtggtc gagcagctcg tcgacaagcg ggtaatcctc aggccgtagg
      421 taggtcgcgt tctcctcata gtgcttgaag tggccctgct cgatcatcgg cgtctcgttg
      481 atgtggtagt cggtcgaaat cccgtggtcg tgcgcgatct cggtgagctc ccggatgtcc
      541 gccatattgt tgcggcagat gttcatgttg aagacgacga tgtagccgta gcgccgctgc
      601 atcttgacca ggtagtcgaa gtacggccgg atgcggtcga gcgccttggg gagccccggc
      661 cgctcctcga cgcaatcgac cgcgaggttg atcgccgcca cccccgcgtc gccgatccag
      721 tcgacgacct gcggcttcat gagccgcccg ttggtcggca ggtacatgaa gaagcccttc
      781 ttcgacccgt aatagatcac cttgtggatg aacttcggcc gcagcagcgg ctcgcccccc
      841 atgatcgccg tcacccggca gccgaccgag tgcaggaagt cgatcgaccg cttggccacc
      901 tcctcggtca tccccttgac gctgttgtca aaggcccagc agtagtggca gtcgaggttg
      961 cacttccact cggtgaagag gtaggagagc aagggccgga actcgttcgg catgacgcgc
     1021 gactggatgt aggggatgag ccagcggcgg aaggcccgga cgccgttgtt ggcgttgtag
     1081 aagagccccc gctgcttgag atccttggtc cgcctgatct gcgggaagat cggctccggg
     1141 agagtcaccg gctcggccaa cggggcgagc gcggagcgcg ccgaggtctt ctgcaggagc
     1201 gtgtagagcg ggacgtcgat gcggaaggtc ggggcggatt cggccgcgac ctgcggtttg
     1261 tcgcctgctg cctgttcgag cattgccggg gactcctttc tcctgtcgaa gacaaatgaa
     1321 aatctgctcg ctcctctggg cctgatccgt atcgatccgc ccgatccctg tcatccatct
     1381 catctggggg ctattcccta cccaacccct gaatctgcac cagcgccggc gcctctgcct
     1441 ccttgaggac gtgctggcgg tagagctgcg ccaggcgctc gtgctcccgc tcccggcgca
     1501 gccgctcggc gcgcgcccgc accttctcct cgcgcgcgtt cttggcgatc cccagctcgt
     1561 ccaggtcgtg ctgctcctcg agcgtcacaa tctcctcccg caggaccatc gagtgcgcca
     1621 ggctgtcgag cccgacactc ttgccgccgg cgaagatctc gtgccgcccg tgctcctcgt
     1681 accacttggt gagcgtcgcc gtcatgtgca tcttctcgac gatgttccgc cagccaactc
     1741 cggtgttgta ggcgcagaag gaaatctctc cctcctgtgt cgcatagggg atgatgcaca
     1801 tctcggtccg gcggaagtcg tagttgaaca ggtcctggaa ccacatcccg gcaatgaaga
     1861 agacgttcca gcggtcccgg cgccgcctct cgatgtcctc gcgcgtccgg tcggccccga
     1921 ccttgccgta gtcgcggccg ctcgccccga agcccttgtc gaacttcatg agcatgtccc
     1981 agaggtggaa gtgcgtcgtc gccttgaacg ggtcgaagtt cttgaccacc gccagcccca
     2041 tgccgaggac cgagaggaac cggccgcgcc ccgcgtcgtt gatcttggcg acgtcgcgcg
     2101 ccagctggta gacgtcgagg aacgaggtca gcgtcgccgc ctccttcgtc tccttgtcga
     2161 ccatgaccga catgccgacg ccgcagttcg ggtggcagcc gcagttgagc tgcccccact
     2221 cgcggtccgg cccgtgcacc aggtcggccc agtcggagaa ggtcccgagg aacgagatcg
     2281 ggaaccagtc gcggcgcggg tcgccgaggc ccgtctgctg cctcacgtcg tgcgccaggt
     2341 gcgagagcgt atagcgctgc gcctgccgcc gctcgtcggt gatcgcctcg tcgcgtccgg
     2401 tgaaggagac gggctggaag gagcagagcg agacgatctt cgggttgtcg agcgcgaact
     2461 tgatgatccg cccgacctgc tcgttgttga tcccgttgac gatcgtcgtc accggcacga
     2521 tgtcgacccc cgcctcgacc agattctcga tcgcccgcat cttgacgtcg aagaggttcc
     2581 ccaccttgcg atggctgttg gccgcgttgc cgatgccgtc aaactggagg taggcgtagc
     2641 gcaaccccgc ctcggccgcc cggcggcaga agtccttgct cttggcgaac tcgatcccgt
     2701 tggtcgccgc ctggacgctg gtgtagccga ccttgcgcga gtactcgatg gcctggatga
     2761 agtggggcga aagcgtcggc tcgccgcccg agaactggac cgagagctgg cgccgcggct
     2821 tgatcgagat cgcgttgtcg agcagagtct ggatctcctc ccagctcaac tcgtggacga
     2881 agccgacctg gttggcgtcc atgaagcacg ggtcgcacat catgttgcag cggttggtga
     2941 ggtcgatggt gagcactgcg ccgcgcccgt gctggatcgt cgaggtgccg tggtcgtgca
     3001 gtttcttgtc gctgtgcgcc cggatgtcgc gcccggggaa tgactcctcg aggtggcgga
     3061 acatctccgg gtcgaccgac atgacgtcct cgaagtggcc gtggaccggg cagtccttga
     3121 ccatgagaat cttgccgtcg cgctcgatga cctgtgcctt gatctcgccg acgcgctccc
     3181 ggcgcaggat ttccaccggc agctcgccgt cgaggatctg ctggcggatc tcggggacgc
     3241 acttcgggca gagcgagtcg gtcgtccgcg gccaaccaag cgg
//
LOCUS       DASRIQ010000507         5838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2018962, whole genome shotgun sequence.
ACCESSION   DASRIQ010000507 DASRIQ010000000
VERSION     DASRIQ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018962"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..271)
                     /locus_tag="VFD06_12340"
     CDS             complement(101..271)
                     /locus_tag="VFD06_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010553058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04362.1"
                     /translation="MESMVVRHEVVKTGITFGTALAMAISFNVNQSVLWAMIHGVCSW
                     LYVIYFVIRWRL"
     gene            complement(303..788)
                     /locus_tag="VFD06_12345"
     CDS             complement(303..788)
                     /locus_tag="VFD06_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04363.1"
                     /translation="MVTTQEQNRITPGIGAALGAFAAFLLLALGAQLFWQRREGLDGA
                     VSTRIEEGTRIEAIAAFCGRRAVSRAPAFERADAAAIAGHLTLDLTEAGLATSGGRVD
                     ALVFGGKLELKVPEDWTVVRGEQVLLGAFVNRTRRSQADPEKILRLDGLVLGGAIVVS
                     H"
     gene            complement(798..1511)
                     /locus_tag="VFD06_12350"
     CDS             complement(798..1511)
                     /locus_tag="VFD06_12350"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HZN04364.1"
                     /translation="MEGERNTLGERSTLNAPRLFLGLVVVTLGLIALLDNLGVIQVDS
                     AWRFWPLFLVAFGTARLLRPAGCPGRWTGFMLVLVGLWLLLQNLGLMPYRLIHFWPVL
                     IVLIGLRLVWGGMQQRAREGAPSASAARISSFAMLGGSEHKSNAADFRGGDVTAILGG
                     AKIDLRNANVKDGDAVIDTFAMWGGIEIIVPRNWSVVVQGTPIMGAYEDKTDQHPDSG
                     GPRLVVRGVVIMGGVEIKN"
     gene            complement(1733..2347)
                     /locus_tag="VFD06_12355"
     CDS             complement(1733..2347)
                     /locus_tag="VFD06_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZN04365.1"
                     /translation="MLRTRAAILALIAVAGFASAPVMAQDNSFKFFVTANWISPLAED
                     DITFEGVSDSIQGSDEFGYEAGFEWRLNKIIGIEGSYMLGSNNFELGSTDIGELDQQT
                     ITAALNVHILPTTFFDLWIAPVASWYTFDDIDTPGDTVEVGSEWGYGAAVGFDIGFGK
                     TFAITGGVRYIKLDVTGDTSGGDIDVGIDPLIARAGIAFRFGSR"
     gene            complement(2482..3105)
                     /locus_tag="VFD06_12360"
     CDS             complement(2482..3105)
                     /locus_tag="VFD06_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HZN04366.1"
                     /translation="MFRVRTVILALAAVIVFASVPALAQDHKHRAFITADWVSPLGTD
                     DIDFESVQDAVQGSDDFGFEAGYEFRFNKLVGLEGSYLIGSNDFELEDADTELGTVDQ
                     QAVTAALNFHILPTKVFDLWLAPIVSWYAFGDFDIDDAIDSEDVELNTEWGYGAQVGF
                     DIGLGKTVAITGGVRYVKVELAGEDFSGIDFDPIIARLGLAFRWGTQ"
     gene            complement(3248..3874)
                     /locus_tag="VFD06_12365"
     CDS             complement(3248..3874)
                     /locus_tag="VFD06_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HZN04367.1"
                     /translation="MFRARTVIPVLAAVAFFASAEAQAQDHKFKFFITADWVSPLGDD
                     EITFGSVQDAVQGSDDFGYEGGFEWRMNKLVGLEVSYLIGSNDFELEEFDTELGTVDQ
                     QAVTAALNFHILPTKVFDLWIAPIASWYTFGDFDFDDAIDSEDVELDSEWGYGAAVGF
                     DIGLGKTVAITGGVRYVKLDLASDGFDNDLAFDPVIARVGLAFRFGER"
     gene            4059..>5838
                     /locus_tag="VFD06_12370"
     CDS             4059..>5838
                     /locus_tag="VFD06_12370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN04368.1"
                     /translation="MAQDPPAPPQGPPAPPRKGGRLRQIKVVAIRPRGTPKDPPPALP
                     ERPQGETKALSAFPDQPVPFSSLDLPELLLRAIEGMGYTQATQVQAAAIPKARGGRDL
                     IVQSRTGTGKTAAFGIPIIEKVDASLKEVQALILTPARELCLQVTTEMERLGEHGGVR
                     ALAIYGGDSMTRQIEGLKRGAQVVVGTPGRLLDLLRQGVLRLEAVTTLVLDEADQMLD
                     MGFEKEVKQILDRVPKSRQTLLFSATIPSEIEAIASRYLKEPERMMLSQDFVYVSDVL
                     HLFYIVTRMGKADALHKLIEWENPTSSMIFCNTRAEVRMVFGSLARYGLPAAMISSDL
                     PQKKREQVMRRFKNKELKHLVATDVAARGIDIEDLSHVFIYSAPESSDQYIHRAGRTG
                     RIGKTGKAISLVSGFDLMNFNRLVRANNLKAYECDLPSDEEVRQREVRRIVVTLKEQA
                     GKLSAEERAEFDPMAQGILDQEDRVAIVAYLLKAHFEEEAARGVLDEAGLPDDDAGTA
                     GAAAEEPSPGGGPGGGRGFGRRGGGHGGHGRSSGAQGRHGGGHRHGGDRGRHGGGDAA
                     PAGQAAPAAHQGSGQAAQGGQEGGTAA"
BASE COUNT         1027 a         1951 c         1970 g          890 t
ORIGIN      
        1 agcagcagcg cctcctccca ccccgccccg ccgccgagcg ccaggatcgt cgccgccagc
       61 cccccgagga gcagggcaat cgtcaggaag agcccgatcc tcacaggcgc cagcggatca
      121 cgaagtagat gacatacagc caggagcaga cgccgtggat catcgcccac agcaccgact
      181 ggttgacgtt gaacgaaatc gccatcgcga gcgcggtgcc gaacgtgatg ccggtcttga
      241 ccacttcatg tcggacgacc atggactcca tggatgcctc cttcgtgccc gggtgacgtc
      301 gatcagtgcg agacgacgat ggcgccgccg aggaccagcc cgtcgagacg caggatcttc
      361 tccgggtcgg cctgcgagcg gcgcgtgcgg ttgacgaatg ccccgaggag cacctgctcc
      421 ccgcggacca cggtccagtc ctccggcacc ttcagctcga gcttgccgcc gaacaccagg
      481 gcgtccaccc gccctccgga cgtcgccagc cccgcctccg tcagatcgag cgtcagatgc
      541 ccggcgatgg cggctgcgtc ggcccgctcg aacgccgggg cgcggctcac cgcgcggcgc
      601 ccgcagaagg cggcgatcgc ctcgatccgc gtcccttcct cgatgcgggt cgagaccgcc
      661 ccgtcgagcc cctcgcggcg ctgccagaac agctgcgcgc cgagggccag caggaggaac
      721 gcggcgaagg cgccgagcgc cgctccgatc cccggtgtga tccgattctg ctcttgagtc
      781 gtgaccatga tcccctccta attcttgatc tcgacgccgc ccatgatgac cacgccgcgg
      841 acgaccaggc gcgggccccc ggaatcgggg tgctgatccg tcttgtcctc gtacgcgccc
      901 atgatcgggg tgccctgcac caccaccgac cagttccgcg gcacgatgat ctcgatccct
      961 ccccacatcg cgaaggtgtc gatcaccgcg tcgccgtcct tgacgttcgc gttgcgcagg
     1021 tcgatcttcg ccccgccgag gatggcggtg acgtcgccgc cgcggaaatc ggcggcgttg
     1081 ctcttgtgct ccgagccgcc cagcatcgcg aacgacgaga tgcgcgcggc gctcgcggac
     1141 ggcgcccctt cccgcgcccg ctgttgcatg ccgccccaga ccaggcgcag accgatgagg
     1201 acgatgagca ccggccagaa gtggatcagc cggtacggca tcaacccgag gttctgcagc
     1261 agcagccaca ggccgacgag gaccagcatg aagccggtcc agcggccggg gcagccggcg
     1321 gggcggagca ggcgcgccgt tccgaaggcg acgaggaaca gcggccagaa ccgccaggcg
     1381 ctgtcgacct ggatcacccc caggttgtcg agcagggcga tgagccccag cgtcacgacc
     1441 acgaggccca ggaacagcct cggggcgttc agggtgctgc gttcgcccag ggtgttgcgt
     1501 tcaccttcca tcgattctcc tcccgacgcg tcgagtcgcg tcatcggggg ggagaatacg
     1561 agggagggaa gggggtgtcg cggtctattc ggcggacgga ggcggggagc cggtgaacag
     1621 cggcacgccg gggctggcag agacggcgcg cgggcgcgag gcggcccgga caggcgaagg
     1681 gcgggtcccc ggagaaggag acccgccctc gatgctcaga acgatgacga cgtcagcggc
     1741 tgccgaagcg gaaggcgatg ccggcgcggg cgatcagcgg gtcgatgccg acgtcgatat
     1801 cgccgcccga ggtgtccccc gtgacgtcca gcttgatgta gcgcaccccg ccggtgatgg
     1861 cgaaggtctt gccgaaaccg atgtcgaagc cgactgcggc gccgtagccc cactccgaac
     1921 cgacctcgac ggtgtcgcca ggggtgtcga tatcgtcgaa cgtgtaccag gaggcgaccg
     1981 gcgcaatcca caggtcgaag aaggtcgtcg gaaggatgtg gacgttgagc gccgcggtga
     2041 tcgtctgctg gtcgagctcg ccgatgtcgg tgctcccgag ctcgaagttg ttgctgccga
     2101 gcatgtagct tccctcgatg ccgatgatct tgttcagcct ccactcgaac ccggcctcgt
     2161 agccgaactc gtcgctcccc tggatcgaat cgctgacccc ctcgaaggtg atgtcgtcct
     2221 ccgccagcgg ggagatccag ttcgcggtga cgaagaactt gaacgagttg tcctgcgcca
     2281 tgaccggcgc ggacgcgaaa cccgcgacgg cgatcagggc caggatggca gcgcgtgttc
     2341 ggagcattcc attcctcctt ggatcgggtt cccacttccg cttgaagacg gggcgggact
     2401 ctaccattcc cggaggaaaa aagggcgggc ccccgggaag gggactcgcc ctctcgaagg
     2461 aatccgttca gcgcggcgcg attactgggt gccccagcgg aacgcgagcc cgagccgcgc
     2521 gatgatcgga tcgaagtcga tgccagagaa atcctcgccg gcgagttcga ccttgacgta
     2581 gcggacgccg ccggtgatgg cgacggtctt gccgagcccg atgtcgaacc cgacctgagc
     2641 gccatagccc cattcggtgt tgagttccac gtcttcgctg tcgatggcgt catcgatgtc
     2701 gaaatcgccg aacgcgtacc aggagacgat cggcgcgagc cagagatcga agaccttggt
     2761 cgggaggatg tgaaagttga gcgccgcggt gaccgcctgc tgatcgaccg tgccgagctc
     2821 cgtgtcggcg tcctccagct cgaagtcgtt gctcccgatc aggtagctcc cttcgaggcc
     2881 aaccagcttg ttgaaccgga actcataccc ggcctcgaac ccgaaatcgt cgctcccctg
     2941 aacggcatcc tgcaccgact cgaagtcgat gtcgtccgtg ccgagcgggg acacccagtc
     3001 cgccgtgatg aacgcgcggt gcttgtggtc ctgcgccagg gcggggaccg acgcgaagac
     3061 gatcaccgcc gccagggcca ggatgactgt gcgcactctg aacatgcgaa tcctccttga
     3121 gttcggggtg atcaccccgc gttgcctgca acaagatgag ggggcccctc gcacatcgct
     3181 ggacggaaga agggcgagcc agggaccggg gacccgtcct cgaatgaatc cgttcagcac
     3241 ggcgcgatca gcgttcgccg aaccggaagg cgaggccgac gcgcgcgatc accgggtcga
     3301 acgccaggtc gttgtcgaaa ccatcgctgg cgaggtcgag cttgacgtag cgcacgccgc
     3361 cggtgatggc gaccgtcttg ccgaggccga tgtcgaaccc gaccgctgcg ccgtaacccc
     3421 attccgagtc gagctcgacg tcttcgctgt cgatggcatc atcgaaatcg aaatcgccga
     3481 acgtgtacca ggaggcgatg ggggcgatcc agagatcgaa gaccttggtc gggaggatgt
     3541 ggaagttgag cgccgcggtg accgcctgct gatcgaccgt gccgagctcc gtgtcgaact
     3601 cctccaactc gaagtcgttg ctgccgatca ggtagctcac ctcgaggccg accagcttgt
     3661 tcatccgcca ctcgaacccg ccttcgtatc cgaagtcatc gctcccctga actgcgtcct
     3721 ggaccgatcc aaatgtgatt tcgtcgtcgc cgagaggcga gacccagtcc gcggtgatga
     3781 agaacttgaa cttatggtcc tgcgcctgag cctctgccga cgcgaagaac gcaactgctg
     3841 ccaggaccgg aatgacagtc cgtgctctga acattcggtt cctcctcccg aagatctctt
     3901 gtcctcccac tacccggttg aagatgtggg caggacaata gcggcgcggt ttttccgcga
     3961 ccatacccag aaatcccgag agacctgtcc gattcgcggc ggcctcatgg gcgcttcgtt
     4021 gaccgctgcc gcgagggggg catagaattc gccccttgat ggcccaggat ccccccgccc
     4081 ctcctcaggg tccacctgcg cccccccgta aagggggccg gctgcggcag atcaaggtcg
     4141 tagcgatccg gccgcgcggc actccgaagg atccgccgcc ggcgctgccg gagcggccac
     4201 agggtgagac gaaggcgctt tcggcgttcc ccgatcaacc ggtgccgttc agttctttgg
     4261 atctgccgga gctgctgctc cgcgccatcg aggggatggg gtacacccag gcgacgcagg
     4321 tgcaggcggc cgcgatcccg aaggcgcgcg gcggccgcga cctcatcgtc cagtcgcgca
     4381 caggcaccgg caagaccgcc gcgttcggca tcccgatcat cgagaaggtg gacgcctcgc
     4441 tcaaagaagt gcaggcgctc atcctgaccc cggcgcgcga gctgtgtctg caagtgacga
     4501 cggagatgga acggctcggc gagcatggtg gtgtgcgcgc gctggcgatc tacggcggcg
     4561 actcgatgac ccgtcagatc gaggggctga agcgcggcgc gcaggtggtc gtcggtaccc
     4621 ccgggcgcct gctcgatctc ctgcgccagg gggtgctgcg gctcgaggcg gtgacgacgc
     4681 tcgtcctcga cgaagcggac cagatgctcg acatggggtt cgagaaggaa gtgaagcaga
     4741 tcctcgaccg cgtcccgaag agccgccaga cgctgctgtt ttccgccacc atcccgtcgg
     4801 agatcgaggc gatcgcgtcc cgctacctca aagagccgga gcggatgatg ctgtcgcagg
     4861 acttcgtcta cgtctccgac gtcctgcacc ttttctacat cgtcacgcgc atggggaagg
     4921 cggacgcgct gcacaagctc atcgagtggg agaacccgac ctcttcgatg atcttctgca
     4981 acacccgggc ggaggtgcgc atggtgttcg gctcgctggc gcgctacggg ctgccggcgg
     5041 cgatgatctc ctccgacctg ccgcagaaga agcgcgagca ggtgatgcgc cgcttcaaga
     5101 acaaggagct gaagcacctg gtcgcgaccg acgtggcggc gcgcggcatc gacatcgaag
     5161 acctgtcgca cgtcttcatc tactcggcgc cggagtcgtc cgaccagtac atccaccgtg
     5221 ccgggcgcac cgggcggatc ggcaagaccg gcaaggcgat ctcgctcgtg tccgggttcg
     5281 atctcatgaa cttcaaccgc ctggtgcggg cgaacaatct caaggcgtac gaatgcgacc
     5341 tgccctccga cgaggaggtg cggcaaaggg aggtgaggcg gatcgtggtc accctcaagg
     5401 aacaggccgg gaagctgtcg gccgaggagc gcgccgaatt cgacccgatg gcgcaaggga
     5461 tcctcgacca ggaggatcgc gtcgcgatcg tcgcctacct cctcaaggcg cacttcgaag
     5521 aggaggcggc gcgcggcgtc ctcgacgagg cggggctgcc cgacgacgac gccgggacgg
     5581 caggagccgc cgcggaagaa ccctctcccg gcggcggccc tggcggcggg cgcggattcg
     5641 gtcgccgcgg cggcggccac ggcggccacg gtcgcagcag cggagcgcag ggccgtcatg
     5701 gcggtgggca ccggcacggc ggcgatcgcg gccggcacgg cggcggcgat gctgcgccgg
     5761 ccggccaggc cgcgcccgcg gcacaccagg gaagcggcca ggcggcgcaa ggcgggcagg
     5821 aaggcggcac cgctgcgg
//
LOCUS       DASRIQ010000508         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3869767, whole genome shotgun sequence.
ACCESSION   DASRIQ010000508 DASRIQ010000000
VERSION     DASRIQ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3869767"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFD06_12375"
     CDS             complement(<1..280)
                     /locus_tag="VFD06_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN04369.1"
                     /translation="MFEKRLVAGWGDMDFNSHMRNTAFLDKSADVRMMFFSENGFPME
                     EFLRRRIGPVIRRDELEYFKEVRLLEELRVTLVMAGLAEDGSRFLIRNE"
     gene            579..1028
                     /locus_tag="VFD06_12380"
     CDS             579..1028
                     /locus_tag="VFD06_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04370.1"
                     /translation="MTLWFHPLCAAYKRPDAILQTLAETSEGVPDRETLERAARGNSA
                     HRRLPRIDGVQRAPNSRAKCRSCREPIERGTWRMRLVFFEEGRFLPGGFVHLDCRGAH
                     FENHDVLDPMLHFSPDLSDDERAELARACGAEGPRAFKAPAPGETPG"
     gene            complement(994..1422)
                     /locus_tag="VFD06_12385"
     CDS             complement(994..1422)
                     /locus_tag="VFD06_12385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN04371.1"
                     /translation="MKSERVFRVLRNELSELRPLSAWVRDIAKGLGLSASTCHDIDLC
                     LHEAVSNIIRHGCRNEVEHRVVVRFETVSAGISIQVEDDARPFNPLAVPLPPEAAALE
                     DARTSGYGLPLMRGLANELSYECVDGRNLLTLVFHPGQGL"
     gene            complement(1486..1839)
                     /locus_tag="VFD06_12390"
     CDS             complement(1486..1839)
                     /locus_tag="VFD06_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN04372.1"
                     /translation="MDCTHQDFEDGVRVIRLTGRMDIEGNERIALPLTVAARSSGGAV
                     VVDLAGVDFLASVGIGTLVSLAQSVQLHKGRLVLCGANEQVRRALERMNMATLIPLYR
                     EVVEARTAIATPSAE"
BASE COUNT          347 a          751 c          719 g          322 t
ORIGIN      
        1 actcgtttcg tatgaggaag cgactgccat cttcagcgag acccgccatg accagcgtga
       61 cgcgtagttc ttccagcaga cggacctctt tgaagtactc gagctcgtct ctccgtatga
      121 ccgggccaat cctccggcgg aggaattcct ccatcgggaa gccgttctcg gagaagaaca
      181 tcatgcggac atcggccgac ttgtccagga aggccgtatt cctcatgtgg gagttgaagt
      241 ccatgtctcc ccagccagcc accagccgtt tctcgaacac ttcgcggact cctgaagacg
      301 aggggtgcag cgccggcccg cgggctcccg ggccgttgca cctaccggga gaggcagtgg
      361 gggaatctac ctcagccggc cggttccgcc gaccccgacc gcccgccgcc gcgccggtgg
      421 tgcgcgggga cggccgtccc gggtatcctc ccatcatgcc ccacgtcttc gagccggctc
      481 ccacgggccg cgccaagtgt cgcggctgcg gcggaccgat ccccaaaggg gagatccgat
      541 tcggcgaacg gatgcccagc ccattcgccg agggcgagat gaccttgtgg ttccaccccc
      601 tgtgcgccgc ctacaagcga cccgatgcca tcctccagac cctcgccgaa acctcggagg
      661 gcgtccccga ccgcgagacg ctggagcgtg cggcgcgcgg gaactcggcg caccggcgcc
      721 tgccgcgaat cgacggggtc cagcgggcgc cgaacagccg tgcgaagtgc cgaagctgcc
      781 gggaaccgat cgagcgcggg acctggcgca tgcgactcgt gttcttcgag gagggccgtt
      841 tccttccagg aggattcgtg cacctcgact gccgcggggc ccacttcgag aaccacgacg
      901 tgctggatcc gatgctccac ttcagtccgg acctgagcga cgacgagcgt gcggagctgg
      961 cgcgggcatg cggtgccgaa ggcccgcgag ccttcaaagc ccctgccccg ggtgaaacac
     1021 cagggtgagc agattccggc cgtcgacgca ctcgtagctg agctcgttgg cgagccccct
     1081 catcaggggc aggccatagc cgctcgtgcg ggcgtcctcg agggccgcgg cctcgggcgg
     1141 gagcggcacg gccagcgggt tgaacggacg ggcgtcgtct tccacctgga tgctgatccc
     1201 cgcggacacc gtctcgaagc gaacgacgac gcggtgctcg acctcgttgc ggcacccgtg
     1261 tcggatgatg ttggaaacgg cctcgtgcag acacaggtcg atgtcgtgac aggtgctcgc
     1321 ggagagtccg agtcccttcg cgatgtcccg tacccacgcg gagagcggcc gcaattcgga
     1381 cagctcgttc cgcaggacgc gaaagacccg ctcggacttc atctcaggtg ctcggcggcc
     1441 ccggaaccgc ggtcatcgca tgagtccgcc acgaaccgcg cccgatcact ccgcggacgg
     1501 ggttgcgatc gcggtccgtg cttcgacgac ttcccggtac aggggaatga gcgtggccat
     1561 gttcatgcgc tccagggcgc gccgcacctg ctcgttggcc ccgcacagga ccagccggcc
     1621 cttgtgcagt tggacggact gggccaggct caccagggtt ccgattccca cggaggcgag
     1681 gaagtccacg cccgcgaggt ctacgaccac cgcgccgccg ctgctcctgg ccgcgaccgt
     1741 gagcggcagg gcgatccgct cattgccctc gatgtccatg cggcccgtga gccggatgac
     1801 gcggacgccg tcctcgaaat cctggtgcgt gcagtccatc cttccctccc gtgtgcccga
     1861 ccggagcgct catcgccgag tcgatcgatc agaatagtga gacgaagggg cgagtcaaac
     1921 cggtccagcc ggggaagccc aggtcgagat cgtggcgtcg gccgctgcgg gccgctcccg
     1981 gcgatcgcgc cgacgcctgg actccgcgcc gatcagccgt ccccgagcct ctcggccgcc
     2041 gccgcgtaga ggacgccacc gaacgccgag ggccgtcggg ccatgatgac cggcggggag
     2101 aggcccgagg cggcggccag cgcctgcagc cgggcggca
//
LOCUS       DASRIQ010000509        10626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3596031, whole genome shotgun sequence.
ACCESSION   DASRIQ010000509 DASRIQ010000000
VERSION     DASRIQ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3596031"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="VFD06_12395"
     CDS             <1..1778
                     /locus_tag="VFD06_12395"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HZN04373.1"
                     /translation="EAGRHLLHAKMTIPAKPGPLVLVYPKWLPGEHGPTGPNVDIVGL
                     SFTAAGKTLPWTRDPADMYQYRLEVPAGAAVVEATLDFAIMTGTGGFTSGGSSTDNLA
                     IVSWNQVILYPAGPNGDDIQFSPSLMLPDGWKSATQLESTGSGTDITYKPVSLTMLVD
                     SPVLAGRFFRTIPLTDPADRHPVRLNIAADSAAALDAKPEVLDRFKRLVAEETTLFGA
                     RHYRKYDFLMALSDHIAHFGLEHHESSDNRLPERFLVDPDIFLIHGDLLAHEMTHSWN
                     GKYRRPRGLQANDYRQPIDSSMLWVYEGLTNYLGDTLAARSGLWTEEQARDHLAWDVA
                     NLSAQPGRRWRPLVDTGTAAQLLYGASDAWQWDRRSVDFYEEGTLLWHEVDSILRERT
                     GGKKSLDDFCRLFHGGADGPPEVKWYDADEVYRTLEGIASYTWKAFFEERVYGLRKEP
                     PLGWLERGGWRLAYSDEKPALLKAREEVDDIADERHSLGFLLRKDAVVVDVFGGSPAA
                     KAGLAPSMTLIAVNGRRYDRKVLETALGDAKSGRGPIEILAENAEFFHTFKVDWSGGE
                     RYPRLERIEGKPDGLSEVLKPRAGR"
     gene            1824..2762
                     /locus_tag="VFD06_12400"
     CDS             1824..2762
                     /locus_tag="VFD06_12400"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN04374.1"
                     /translation="MTVPRTALPGLFVLLLLAPGSLCLAQDAAPAVGAAPVPAPTAPA
                     AATTTGRILGRVTDAGKPVQGAQVRLVSRSESGLLRVTSTDERGEYRFKELPTGIYDV
                     EVEVDGFRPGAKPGVEVKAPFQNIVDVPLSRTGAPGKLPIRPPVPGAPEQGVPGRAAD
                     PGQQQEPPPAPAVVRGTLRDAARNPVVDVSVLLVAQAGTRLYQAISGSDGAFTIEGVI
                     PGKYRAVVRSPGHMPVDLKSVEVRPVAGLTLNLAVVDFPLNFKARADGSPPPEVPKPL
                     PGSSGDSSAPSAPPASPPAGAAVPASPPADATDSGG"
     gene            complement(2769..4061)
                     /locus_tag="VFD06_12405"
     CDS             complement(2769..4061)
                     /locus_tag="VFD06_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN04375.1"
                     /translation="MNRGALARSSIRRSKAALMALGCALAIAFAVTPARTQSTPVPPP
                     QSAPAAPPGAPAPAPKAPEVKSRRGFSIAITNPVMNDFRYGRSEIEVDVKASDPAATE
                     KVEFYVDEKLIFIDTEPPYRCVFDFGTEPRSWVIKATAFHREGLTVSDQVILRKVIIN
                     YAVQVNRVILYSTAMSQKDNKKYVLDLEKKDMVLEEDGVKQEIIDFYREERPVTLAVV
                     SDSSGSMQAAMPIVHNAASRFIDTLGAEDKALVIDFDDKVYLLQDVTSDKKDLRVALT
                     STNALGGTALYDALYASYRKLKGVDGRKAIILLSDGDDTASKFSFRRVLDEAKINDIL
                     IYSIGLGTGFLDVDLRRVLKSLAEETGGHAYFPDKPDELEAVYREISLELKSQYYATY
                     EPQNTTWDGRWRKIRLTAPGRDVEMRTRSGYYAIRKPG"
     gene            complement(4058..5692)
                     /locus_tag="VFD06_12410"
     CDS             complement(4058..5692)
                     /locus_tag="VFD06_12410"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04376.1"
                     /translation="MPPGRFRAFPPFTMPLARAVWVTAAAAVAAFALLRPSPADAMAA
                     QDARTQSFKLQSDGMRLYREGKYPEAIVAFKQVVNIHLNSFMAWYYLGVALSAERRYA
                     EAIEPLKIALDLQPDHLQGHMALGDALLKLGEVDECRAAYLRALDLQANYAQAHDGLG
                     RLHESMGRDAEAEAEYRKALEINVAFADAYTHLGELFLRQGRLDEATALFGKAIAMKP
                     DFSEAYVRLGVALSRLGRFDDAIAAARKSRSLAPRDPEPILALAKIDLEMQSFRRARA
                     GVDEAMAIDQEFVYSHVMLADLARAQGNLPYAVSILEQRLERPIDDPRLKRDIANRLK
                     QARADAKTLAGLEAAEAQAAAATPADPAATVALARFWSGQRNHRMAVDLLLAAADRVQ
                     AGGGAVEPLRFEAGLEQYAARRYAEAIATFEVLAFADEGRPLRDEARFNLGVAQAGAG
                     LDEEAVATFRDCSARAESDPQPRLYLGNALLRLGRPAEAREAYAAYLERAGRGPEADQ
                     VRRLLQRLAGSQAEASAPVADSPTVPGAVAAGKGSR"
     gene            5766..6641
                     /locus_tag="VFD06_12415"
     CDS             5766..6641
                     /locus_tag="VFD06_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04377.1"
                     /translation="MPRAARLARPLPAALAVVAIVVGGAAPVHAVTPDLTFGLRWEQG
                     PFLGVEDAGLGEAYVLIEGAGEMSRLSLRVPYARIDRTGLVTMASDAPILLGAGGPGK
                     PPWQETDAESSASDLGDLMLRLENYIVRSGGGNRPALSFILDYKYGLADEKKGLGTGE
                     SDWGGGLEYVQPLGKVLQFLVTGLYHFMGSPEGVEFEDRLHLMGGFSIVGRRTVWRIV
                     GEHVTPALEEVPVFDTVGVPTGALFEVEDYRVVRGDFVYRSQAGGSTRLYVLGGLNDS
                     SPDIGFGLSFSSRGQ"
     gene            complement(6669..8810)
                     /locus_tag="VFD06_12420"
     CDS             complement(6669..8810)
                     /locus_tag="VFD06_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HZN04378.1"
                     /translation="MAKDRGPEKQAKGGARVLEEAPPDLKDLQARGLSRQTLIALYRN
                     MVLSRRIDDKEIALKRQNKIYFQISGAGHEAILTATAVHLKASHDWFYPYYRDRALCL
                     WLGMTPLEMFLSAVGGADDPSSGGRQMPSHWGHRKLRIVSQSSPTGTQFLQAVGCAEA
                     ERHMSPKGDAIVYVSGGDGATSEGEFWEAVNAASLRKAPVLFLITDNEYAISVPVEAQ
                     TAGGSISAALRGIPGLAIEEVDGCDPIASYEVMRRVVERLRKPGAGPALVHAKTIRPY
                     SHSLSDDEAMYRPKRELQDETSRDPIPNFAAYLKKIKAVDEAFLDAVRSEVDREVEEA
                     AQAALAAPRPDPSTIHQHVWSPTVDPTSSQFDVPPKIEGDPKTMVDLLNACLRDEMAR
                     DETIVVFGEDVADCTREESLPEVKGKGGVFKVTHNLQRKFGSARVFNTPLAEAMILGR
                     AIGMATRGLKPVVEIQFFDYIWPAMMQLRNELAAMRWRSNGHWACPLVVRVPIGGYLQ
                     GGAIYHSQCGETLFTHTPGVRVVFPSNALDANGLLRTAIRCDDPVMILEHKHLYRQTY
                     NKSPYPGPDHCIPFGKARLVREGKDVTVVTYGALVERSLRAAKALAEAGTEVEILDLR
                     SLWPLDWEAIAESVKKTGRLLVAHEDTLSFGYGAEIAALAADRLFHHLDAPIRRVAAT
                     DTFVAYEPGLEDAILPQVETLKAAMTEIAAL"
     gene            complement(8840..10111)
                     /locus_tag="VFD06_12425"
     CDS             complement(8840..10111)
                     /locus_tag="VFD06_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZN04379.1"
                     /translation="MKRFPAFDPPEYVGFRPDPKVTAEYSATLRRDPKRRALVERLPR
                     AEHLRLYEGMVRFRLHDITLKRWVRQGVISKAWLGTGEEAVAVGTTRALRPGDFVGPM
                     IRNAGSCHEMGMPVAQMLKAYLGTHDQITRGRDLHTGDPEKGVIPPTSFMAALVPVFT
                     GIALAAKQRKEPRVALTYVGDGASRTAAFHEGFNFAAVQRVPLVIVLQDNQVALGTAT
                     PRHTAAPMEIQHTAYGVEGLSCDGNNVLDTWAASRLLVDRCRKGDGPFILTARTFRMG
                     GHATHDEAEARALFPPETFAHFGQRDPIGTYEAWLVAAGPALDGRKHRSDVARREANE
                     AALAEAERRVTAEIEAAEAEALRSRKEKMPPPEDAVTGVYAVTTSPATKRGNGRGTAT
                     TKRAAPRSKPRKTVAAHARRTMASRGGRRLS"
     gene            complement(10149..>10626)
                     /locus_tag="VFD06_12430"
     CDS             complement(10149..>10626)
                     /locus_tag="VFD06_12430"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZN04380.1"
                     /translation="ASAHKPDTATLVPERREELTTEGGLDVVLNQNLVRKTALDLREA
                     GVTVSVFVDPDYEQIRAAAKLEIRMIEINTAKYSEARREDQEAAEADKVSAAARAAAK
                     LGLRVCAGHGLDYRNVSRIVAIQEIEEMNIGHAIVARAALVGMERAVREMKTLLAR"
BASE COUNT         1492 a         3728 c         3747 g         1659 t
ORIGIN      
        1 gcgaggcggg gcgccacctg ctgcacgcga agatgaccat cccggcgaag ccggggccgc
       61 tcgtcctcgt ctacccgaag tggctcccgg gggagcacgg gccgaccggg ccgaacgtgg
      121 acatcgtcgg gctctccttc accgcggcgg gaaagacgct cccctggacg cgggatcccg
      181 ccgacatgta ccagtaccgg ctggaggtcc cggccggcgc cgcggtcgtc gaggcgacgc
      241 tcgacttcgc catcatgacc ggcaccggcg gcttcacctc ggggggatcg tccaccgaca
      301 acctcgcgat cgtgtcgtgg aaccaggtca tcctgtaccc ggccggaccg aacggcgacg
      361 acatccagtt ctcgccgagc ctcatgctcc ccgacggctg gaagtcggcg acacagctcg
      421 aatccaccgg aagcgggacg gacatcacct acaagccggt gagcctgacg atgctcgtgg
      481 actcgccggt cctcgcgggg cgcttcttcc ggaccatccc cctgaccgac ccggccgaca
      541 ggcacccggt gcggctgaac atcgccgccg acagcgccgc ggcgctcgac gcgaagccgg
      601 aggtgctcga ccgcttcaag cggctggtgg ccgaagagac gacgctcttc ggggcgcgcc
      661 actaccgcaa gtacgacttc ctcatggcgc tgagcgatca catcgcccac ttcggcctcg
      721 agcaccacga atcgagcgac aaccggctgc cggaacgctt cctcgtcgat ccggacatct
      781 ttctcatcca cggcgacctt ctggcgcacg agatgaccca ttcgtggaac ggcaagtacc
      841 gccggccgcg cggtctgcag gcgaacgact accgccagcc gatcgacagc agcatgctgt
      901 gggtctacga ggggctgacg aactacctcg gcgacacgct cgcggcgcgc agcggcctgt
      961 ggacagagga gcaggcgcgc gaccacctcg cctgggacgt cgcgaacctg agcgcgcagc
     1021 cgggacggcg ctggcggccg ctcgtggaca ccggcactgc ggcgcagctt ctctacggtg
     1081 cgtccgacgc ctggcagtgg gaccgccgca gcgtcgactt ctacgaggag gggacgctcc
     1141 tctggcacga ggtggacagc atcctgcgcg aaaggaccgg cgggaagaaa tcgctcgacg
     1201 atttctgccg cctcttccac ggcggcgccg acgggccgcc cgaggtgaag tggtacgacg
     1261 ccgacgaggt gtaccggacg ctcgagggga tcgcctccta cacctggaag gcgttcttcg
     1321 aagagcgggt ctacggcctg cgcaaggaac cgccgctcgg ctggctcgag cgcggcggct
     1381 ggcggctggc ctacagcgac gagaagccgg cgctcctcaa ggcgcgcgaa gaggtggacg
     1441 acatcgccga cgagcggcac tcgctcggct tcctcctgcg caaggacgcc gtggtcgtgg
     1501 acgtcttcgg cggctccccg gcggcgaagg ccggcctcgc gccgagcatg accctgatcg
     1561 cggtcaacgg ccgccgctac gacaggaagg tcctcgagac ggcgctgggc gatgcaaaga
     1621 gcggccgggg gccgatcgag atcctcgcgg agaacgccga gttcttccac accttcaagg
     1681 tggactggag cggcggcgag cgctacccgc gcctcgagcg gatcgagggg aagccggacg
     1741 ggctgagcga ggtcctgaag ccgcgggcgg gccgctgagc ctcgggtttg ctcacggccg
     1801 ccgcacctca taacatggcc gttgtgaccg tcccgcgcac cgccctgccc ggcctcttcg
     1861 tcctgcttct tctggcgccg ggatcgctct gcctggctca agacgcggcc ccggccgtcg
     1921 gcgccgcccc ggtccccgcc ccgactgccc ctgcggcggc aacgacgacc ggccgcatcc
     1981 tcgggcgggt cactgacgct ggcaagccgg tacagggggc gcaggtgcgg ctcgtgtccc
     2041 gctccgagtc cgggctcctg cgcgtcacca gcaccgacga gaggggggag taccgcttca
     2101 aggagctgcc gaccgggatc tacgacgtcg aggtcgaggt ggatggattc cgcccgggcg
     2161 ccaagccggg ggtggaggtg aaggcgccgt tccagaacat cgtggacgtg ccgctgagcc
     2221 gtaccggcgc accgggaaag ctgccgatcc ggccgccggt ccccggcgcg ccggagcagg
     2281 gcgttccggg acgcgcggcc gatccggggc agcagcagga gccgcctccg gccccggcgg
     2341 tggtgcgcgg gacactgagg gacgccgcgc ggaacccggt cgtggacgtc tcagtgctcc
     2401 tggtggcgca ggcggggacg aggctctacc aggcgatttc agggagcgac ggcgccttca
     2461 cgatcgaagg ggtcatcccc ggaaagtacc gcgccgtcgt gcggtccccc ggccacatgc
     2521 cggtcgatct caagtcagtg gaggtgcggc cggtcgcggg gctgacgctc aacctggcgg
     2581 tcgtcgattt cccgctcaac ttcaaggcgc gcgccgacgg ctcgcctccc cccgaggtcc
     2641 cgaagccgct tcccggctcc tcaggagatt cttccgcgcc gtccgcccct cccgcctcgc
     2701 cgccggcggg ggcagcggtc cccgcgtcgc cgccggcgga cgcgaccgat tcgggcggtt
     2761 aggagcgatt agcccggctt acggatggcg tagtagccgc tgcgcgtgcg catctcgacg
     2821 tcgcgcccgg gggcggtgag ccggatcttg cgccagcggc cgtcccacgt ggtgttctgc
     2881 ggctcgtagg tggcgtaata ctggctcttg agctccagcg agatctcccg gtacaccgcc
     2941 tccagctcgt ccggtttgtc ggggaagtag gcgtgcccgc cggtctcctc cgcgaggctc
     3001 ttgaggacgc ggcgcagatc gacgtcgagg aagccggtgc cgagcccgat cgaatagatc
     3061 aggatgtcgt tgatcttcgc ctcgtcgagg acccgccgga aggagaactt gctcgcggtg
     3121 tcgtcgccgt ccgagaggag gatgatcgcc ttgcggccgt ccactccctt cagcttccgg
     3181 tacgaagcgt agagcgcgtc gtacagcgcg gtgccgccca gggcgttggt gctggtcagc
     3241 gccacgcgca ggtccttctt gtcggaggtc acgtcctgca ggagatagac cttgtcgtcg
     3301 aagtcgatga cgagcgcctt gtcctcggcg ccgagcgtgt cgatgaagcg gctcgccgcg
     3361 ttgtgcacga tcggcatcgc cgcctgcatg ctgccgctgc tgtcgctgac gacggcgagg
     3421 gtgaccggcc gctcctcccg gtagaagtcg atgatctcct gcttcacgcc gtcctcttcg
     3481 agcaccatgt cctttttctc gaggtcgagg acgtacttct tgttgtcctt ctgactcatg
     3541 gcggtggagt acaggatgac ccggttcacc tgcacggcgt agttgatgat caccttgcgc
     3601 aggatgacct ggtcggacac ggtcagccct tcgcggtgga acgccgtcgc cttgatgacc
     3661 cacgagcgcg gctcggtccc gaagtcgaag acgcagcggt acggcggttc ggtgtcgatg
     3721 aagatgagct tttcgtccac gtagaactcg accttctcgg tggcggccgg gtcgctcgcc
     3781 ttcacgtcca cttcgatctc ggagcggccg tagcggaagt cgttcatcac cgggttggtg
     3841 atggcgatgg agaagccgcg ccgtgacttg acctcgggcg ccttcggggc cggggcaggg
     3901 gcgccgggcg gtgcggccgg ggcactctgc ggcgggggga ccggcgtgga ctgggtgcgg
     3961 gccggcgtga cggcgaaggc gatcgcgagc gcgcagccca gcgccatgag cgccgctttg
     4021 gatcggcgga tcgaagacct cgcgagcgcc ccgcgattca ccgggatccc ttcccggccg
     4081 cgacggcgcc cgggacggtg ggggaatcgg cgaccggggc cgaggcctcg gcctgcgaac
     4141 cggcgagccg ctggaggagc cggcggacct ggtccgcctc gggcccccgg ccggcccgct
     4201 cgagataggc ggcgtatgcc tcgcgcgcct ccgccgggcg gcccagccgc agcagggcgt
     4261 tgccgagata gaggcgcggc tgcggatcgg actcggcgcg cgcggaacag tcgcggaacg
     4321 tggccaccgc ctcctcgtcg aggccggccc ccgcctgcgc cacaccgagg ttgaagcggg
     4381 cttcgtcccg caacggacgc ccctcgtcgg cgaaggcgag gacttcgaac gtcgcgatcg
     4441 cctcggcgta gcggcgcgcg gcgtactgct cgagcccggc ctcgaagcgg agcggctcga
     4501 ccgcgccgcc gccagcctgc acacggtcgg ccgcggcgag aagcagatcc accgccatcc
     4561 ggtggttgcg ctgccccgac cagaagcggg cgagcgccac cgtcgccgcc gggtcggccg
     4621 gggtcgcggc ggcggcctgc gcctccgccg cctccagccc ggcgagggtc ttcgcgtcgg
     4681 cgcgcgcctg cttcaggcgg ttggcgatgt cccgcttgag ccgcgggtcg tcgatcggcc
     4741 gctccagccg ctgctccaga atcgacaccg catacggcag gttcccctgg gcccgggcca
     4801 gatcggcgag catgacgtgg gagtagacga actcctggtc gatcgccatc gcctcgtcca
     4861 cgccggcgcg ggcccggcgg aacgactgca tctcgagatc gatcttggcg agcgccagga
     4921 tcggctccgg gtcgcgcggc gccaggctcc tcgacttgcg cgccgccgcg atggcgtcgt
     4981 cgaagcgccc cagacgcgac agcgccacgc cgagccgcac gtacgcctcg ctgaagtccg
     5041 gcttcatggc gatcgccttg ccgaacagcg cggtcgcctc gtccaggcgc ccctggcgca
     5101 ggaacagctc gccgagatgg gtgtaggcgt cggcgaaggc gacgttgatc tcgagcgcct
     5161 tgcggtactc cgcctccgcc tcggcgtcgc gccccatgct ttcatggagc cgcccgagcc
     5221 cgtcgtgcgc ctgcgcgtag ttcgcctgga gatccagcgc ccggagatac gcggcgcggc
     5281 actcgtccac ctccccgagc ttcaggagcg cgtcgccgag cgccatgtgc ccctgcaggt
     5341 ggtccggctg cagatcgagg gcgatcttga gcggttcgat cgcctccgcg tagcgccgct
     5401 ccgccgagag cgccacgccg aggtaatacc aggccatgaa cgagttgagg tggatgttga
     5461 cgacctgctt gaacgcgacg atcgcctccg ggtacttccc ttcgcggtag agccgcatcc
     5521 cgtccgactg aagcttgaac gactgagtgc gggcgtcctg cgcggccatc gcgtcggcgg
     5581 gggagggacg gagcagcgcg aacgcggcga cggccgcggc ggcggtgacc cagacggccc
     5641 tcgcgagcgg catcgtgaag ggggggaagg cgcggaaacg accggggggc atcgtgacaa
     5701 aaacctcacg gccactatag cccaaaccgc agagcgcctc cgaactcccc tataatcccg
     5761 gccgcatgcc gagagctgcc cggctcgcca gaccccttcc cgccgctctg gccgttgtcg
     5821 ccatcgtggt gggtggcgcc gcgccggtgc atgcggtgac gccggacctc acgttcggcc
     5881 ttcgctggga acaggggccg tttctcggcg tcgaggatgc ggggctcggc gaggcctacg
     5941 tgctcatcga gggggcaggc gagatgtcgc gcctcagcct gcgggtcccc tacgcccgca
     6001 tcgaccgaac cgggctcgtc acgatggcct ccgacgcgcc gatcctcctc ggcgccggcg
     6061 gccccgggaa gccgccctgg caggagaccg acgcggagtc ctcggcgagc gacctcggcg
     6121 atctcatgct gcgcctcgag aactacatcg tgcgatccgg cgggggcaat cgccccgcgc
     6181 tgtcattcat cctcgactac aagtacggcc tcgccgacga gaagaaggga ctgggcaccg
     6241 gcgagagcga ctgggggggc ggcctcgagt acgtccagcc gctcggcaaa gtcttgcagt
     6301 tcctggtgac cggcctgtat cacttcatgg gctcgccgga gggggtcgag ttcgaggatc
     6361 ggctccacct catgggcggg ttctcgatcg ttggcaggag gacggtctgg cggatcgtcg
     6421 gcgagcacgt cacgccggcg ctcgaggagg tgccggtctt cgacacggtc ggcgtcccga
     6481 ccggcgccct gttcgaggtc gaggattacc gcgtcgtgcg cggcgatttc gtgtaccggt
     6541 cccaggccgg cggctcgacc cgcctctacg tcctcggcgg cctcaacgac tcctccccgg
     6601 acatcgggtt cggcctgtcg ttctcctcgc ggggacagtg atcgcgcgct gctcggacag
     6661 gctcctcgct agagcgcggc gatctccgtc atcgccgcct tgagcgtctc cacctgcggg
     6721 aggatggcgt cctccagccc cggctcgtag gcgacgaagg tgtcggtggc ggcgacgcgg
     6781 cggatcggcg cgtcgaggtg gtggaagagg cgatcggcgg ccagggccgc gatctcggcg
     6841 ccgtagccga acgaaagcgt gtcctcgtgg gcgacgagga gcctgccggt cttcttcacc
     6901 gactccgcga tcgcctccca gtcgagcggc cagagcgagc gcaggtcgag gatctccacc
     6961 tcggtccccg cctccgcgag ggccttcgcg gcccgcagcg accgctccac gagcgcgccg
     7021 taggtcacga ccgtcacgtc cttcccctcg cgcaccaggc gggccttgcc gaacgggatg
     7081 cagtggtccg ggccggggta ggggctcttg ttgtaggtct gccggtacag gtgcttgtgc
     7141 tccaggatca tgaccgggtc gtcgcagcgg atggcggtgc gcagcaggcc gttggcatcc
     7201 agcgcgttcg acggaaagac gacacgcacg cccggcgtgt gcgtgaagag cgtctcgccg
     7261 cactggctgt ggtagatggc gccgccctgc aggtacccgc cgatcggcac gcgcacgacg
     7321 agcgggcagg cccagtggcc gttcgagcgc cagcgcatcg ccgccagctc gttccgcaac
     7381 tgcatcatcg ccggccagat gtagtcgaag aactggatct ccacgaccgg cttcaggccg
     7441 cgcgtcgcca tgccgatggc gcgcccgagg atcatcgcct cggccagcgg ggtgttgaag
     7501 acgcgcgccg agccgaactt gcgctggagg ttgtgcgtca ccttgaagac gccccccttc
     7561 cccttcacct cggggagcga ctcctcgcgg gtgcagtcgg ccacgtcctc gccgaagacg
     7621 acgatcgtct cgtcgcgcgc catctcgtcg cgcaggcagg cgttgagcag atccaccatc
     7681 gtcttcgggt ccccctcgat cttcggaggg acgtcgaact ggctcgacgt cggatcgacg
     7741 gtgggagacc agacgtgctg gtggatggtc gaaggatcgg ggcggggcgc ggcgagcgcc
     7801 gcctgcgccg cctcctcgac ctcccgatcc acctcgctgc ggaccgcgtc gaggaacgcc
     7861 tcgtccaccg ccttgatctt cttgagatac gccgcgaagt tcgggatcgg atcgcggctc
     7921 gtctcgtcct gcagctcgcg cttcggccgg tacatcgcct cgtcgtccga cagcgagtgg
     7981 gagtacggcc ggatcgtctt cgcgtgcacc agggcggggc cggcgccggg cttgcgcagc
     8041 cgctcgacga cgcggcgcat cacctcgtac gaggcgatcg ggtcgcagcc gtccacctct
     8101 tcgatcgcca ggcccgggat tccccgcaac gccgcggaga tgctgccgcc cgcggtctgg
     8161 gcctccaccg gcaccgagat ggcgtattcg ttgtcggtga tgaggaacag gaccggcgcc
     8221 ttccggagag acgcggcgtt gactgcctcc cagaattccc cctcgctcgt cgcgccgtcc
     8281 ccgccggaga cgtacacgat ggcgtcccct ttcgggctca tgtgccgctc cgcctcggcg
     8341 cagccgaccg cctgcaggaa ctgggtgccg gtcgggctcg actgcgagac gatccggagc
     8401 ttccggtgtc cccagtggct cggcatctgc cgcccgccgg agctcggatc gtcggcgccg
     8461 ccgaccgccg acaggaacat ctcgagcggc gtcatcccga gccacaggca gagcgcccgg
     8521 tcgcggtagt aggggtagaa ccagtcgtgc gacgctttga gatggacggc ggtggcggtg
     8581 aggatcgcct cgtgcccggc gccgctgatc tggaaataga tcttgttctg acgcttgagg
     8641 gcgatctcct tgtcgtcgat ccgccgcgac aggaccatgt tgcggtagag ggcgatgagg
     8701 gtctgccggg agaggccgcg cgcctggaga tctttgaggt ccgggggcgc ctcctcgagg
     8761 acgcgggcgc ctcctttggc ctgcttctcc ggacctcgat ccttcgccat gcgcgatccc
     8821 ccctcccaga gcgtggcgcc tatgatagcc gccggccgcc ccgggacgcc atcgtccggc
     8881 gcgcgtgcgc tgcgaccgtc ttgcgcggct tcgaccgcgg cgcggcacgc ttcgtcgttg
     8941 ccgtcccgcg tccgttccct cgcttggtgg ccggagaggt cgtcaccgcg tacacaccag
     9001 tgacggcgtc ctcgggcggc ggcatcttct ccttgcggct cctgagcgcc tccgcctcgg
     9061 ccgcctcgat ctcggccgtg acccgccgct ccgcctcggc cagcgccgcc tcgttcgcct
     9121 cgcgccgggc gacgtcggag cggtgctttc gcccgtcgag cgccggacct gcggcgacga
     9181 gccacgcctc gtaggtgccg atcgggtcgc gctgcccgaa gtgagcgaac gtctcgggcg
     9241 ggaagagggc ccgcgcctcc gcctcgtcgt gcgtcgcgtg ccctcccatc cggaaggtcc
     9301 gggcggtcag gatgaacggg ccgtccccct tgcggcagcg atcgacgaga aggcgcgaag
     9361 cggcccaggt gtcgaggacg ttgttgccgt cgcacgagag cccttcgacg ccgtacgccg
     9421 tgtgctggat ctccatcggc gccgccgtgt ggcgcggcgt cgccgtcccg agcgccacct
     9481 ggttgtcctg cagaacgatg acgaggggca cccgctgcac ggcggcgaag ttgaaccctt
     9541 cgtggaaggc ggcggtgcgg ctggcgccgt cgccgacgta ggtcagcgcg acgcggggct
     9601 ctttgcgctg tttcgcggcg agcgcgatgc cggtgaagac cggcacgagc gccgccatga
     9661 acgacgtcgg cggaatgacc cccttctccg gatcgccggt gtgcaggtcg cgacccctcg
     9721 taatctggtc gtgcgtgccg aggtacgcct tgagcatctg cgccaccggc atccccatct
     9781 cgtggcagga gccggcgttg cggatcatcg gcccgacgaa gtcccccggg cgcagtgccc
     9841 gcgtcgtccc gactgccacc gcctcctcgc cggtgccgag ccacgccttg gagatcactc
     9901 cctggcgcac ccagcgcttg agggtgatgt cgtgcaggcg gaagcgcacc atcccctcgt
     9961 acagtcgcag gtgctcggcg cgggggagac gttcgaccag ggcccggcgc ttcgggtcgc
    10021 gcctcaaggt cgcggagtac tccgccgtga ctttggggtc gggacggaag ccgacgtact
    10081 ccggtgggtc gaacgcgggg aaacgcttca tctcttctcg atcggccggc ggccggggag
    10141 ggcggggttc accgcgcgag caaagtcttc atctcgcgca ccgcccgctc catcccgacg
    10201 agcgcggcgc gggcgacgat cgcgtggccg atgttcatct cctcgatctc ctggatggcg
    10261 acgatgcggg agacgttccg gtagtcgagg ccgtgcccgg cgcacaccct cagccccagc
    10321 ttggcggcgg cgcgcgccgc cgcggagacc ttgtccgcct cggccgcctc ctggtcctcc
    10381 cggcgcgcct cggagtactt ggcggtgttg atctcgatca tccggatctc gagcttggcg
    10441 gcggcccgga tttgctcgta atcggggtcg acgaagaccg acaccgtcac cccggcctca
    10501 cgcaggtcga gggcggtctt gcgcaccagg ttctggttca ggacgacgtc gagacccccc
    10561 tcggtcgtca gctcctcgcg ccgctccggc acgagggtcg ccgtgtccgg cttgtgggcg
    10621 gaggcg
//
LOCUS       DASRIQ010000510         5436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_734610, whole genome shotgun sequence.
ACCESSION   DASRIQ010000510 DASRIQ010000000
VERSION     DASRIQ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734610"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..781
                     /locus_tag="VFD06_12435"
     CDS             305..781
                     /locus_tag="VFD06_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04381.1"
                     /translation="MRDSRTMTAALAVMMAAAALFACAGGGDAKDKQGDAVPAGDASA
                     TPPGQDHANPAPPAAAEPEHYDPASASGAIKGVPAPTNTATIEAVANYFAEVPGFDLA
                     PLGERGREKFLHRVNSELCTCGCKNETIAWCLVNDPKCPMVKGLAQAVYDEVKKGS"
     gene            815..2914
                     /gene="fusA"
                     /locus_tag="VFD06_12440"
     CDS             815..2914
                     /gene="fusA"
                     /locus_tag="VFD06_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZN04382.1"
                     /translation="MKVYDISDIRNVGLIGHGSSGKTSLASALLFASGAVNRLGKVDQ
                     GTTVTDHDEEEIARKVSISSALCHAEWRKTKINLIDTPGYGAFIADARACLRVADGAV
                     LLVDGVSGVEVQTEEAWGFAQEFNLPVLIAVSRLDRENASLERAMASITEAFGRQAVP
                     IQVPMGSERAFKGVIDLVSLKGALYGDDESGKFQVLDAPSPLPPEIKAARERLMEAVA
                     EVDDALMEKFFSAGELSQEDLVAGLTRGIRARRIFPVLCTAGAHNIATHGMLDAIVSY
                     LPHPGERGPFAGENPKDHSALERKGATDEPYSAYVFKTIADPYSGKISMFRVYSGVLK
                     SDSSIHNASRDLAERFGSIASMQGKEMQPVPEVRSGDIAAVAKLKETATGDTLAEKAH
                     PVLYPKLVFPEPSISFALNPKSRADEDKISQVLHRVVEEDPTIQYRRDPQTHELILSG
                     NSDLHVEVVLAKMKKKFGLEAVLHPPKVPYLETIKKKAEAQGRHKKQTGGHGQFGDCW
                     IKLEPLQRGGGFEFVDDIFGGSIPKNFIPSVEKGIQEARSHGYLAGYPVVDFRVVLFD
                     GSYHTVDSSDIAFKIAGSIAFKAAMADAGATLLEPVMNVEIVAPEQFMGDLMGDLNSR
                     RGHVQGMSTQGRSQVIKASVPLVEMLSYASILKSITGGRGSYRMTMRGYEEVPAHQQA
                     KIIAAHKAQKEKEAAAH"
     gene            3159..4163
                     /locus_tag="VFD06_12445"
     CDS             3159..4163
                     /locus_tag="VFD06_12445"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZN04383.1"
                     /translation="MAYDVVFAVGEDPTSFLAKHQEAIRPHARVPLPPLERFMVCRDK
                     SLTMREARRLGIATPQTHDPDAEPLDAFARRVGFPAVVKPNVSDGARGISYPGDGPAL
                     ARALASTEKEYGACHVQEYVPQTGTQFKAEVLLDGASRPRAWCVYSKLRYYPPSGGSS
                     TLNRTVDRPDILDAAARMLQGIGWTGLGDCDFIEDPRDGVPRLMEINPRFTRSIRICV
                     RAGVDFPLLLYRLAVGAGAIPESPGSYRVGVMQRYLPADVAWFLRSPDRFRARPGFFA
                     TFFTGAADEIFSLADPGPALAYLLGAASDLLDPEARRHRLRPKAGASPTGPSSGAAAG
                     "
     gene            4163..5170
                     /locus_tag="VFD06_12450"
     CDS             4163..5170
                     /locus_tag="VFD06_12450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN04384.1"
                     /translation="MLTFLAVVVALLLLGAASFVLSVHAQEHRPDRIPALLYHRLISK
                     ERYDRGEIRSEERSYVVFDAFFAAQMEALQRAGYTTLHLDDYLKIRRGEMARPERPIL
                     VTFDDGFRSNYLYAFPVLRRLGMKATIFMTPDRSSRNFEKNAPLDGPLSDDELREMSA
                     AGVAIESHGMTHRYLTDLDDHTIRWELAESRRVLEATLGRPVRFLAIPSGAYNGTIRR
                     LAQDTGYAAVFSMGKGTMNMKSDLLALKRLVVGRDLDAAGLLSLLEPGNIFLLRLTST
                     VQVTFMRVFGVHTLDRLRALILKLPFGAALIRRPAVLLAAGLSTLAVLGALLVSLLWR
                     S"
     gene            5216..>5436
                     /locus_tag="VFD06_12455"
     CDS             5216..>5436
                     /locus_tag="VFD06_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04385.1"
                     /translation="MTASLVLFWGAAAALAAGHLLVPLTLRALRPWLRRPLEKRPIEP
                     SVSVVISASNEERHITAKIEDCLRFDYPSS"
BASE COUNT          833 a         1997 c         1796 g          810 t
ORIGIN      
        1 gccccggtct cgcggcgccg cccgtgcagg taccccgcgc actcgtagcg ggacagggcg
       61 gtccgcaccg aggcagtctc ccccccggcg ggcgcgccgc cgcgcagggc cgcgacgatc
      121 gcctcctgga tctccggcgc gggcgaaaaa gcgggtgaca ccgacgcatt atagcggccg
      181 cccggatcgg ggggtcggcc gatccggcgc ggaggcccgc tccacggccg attgcaggga
      241 gccctccgct tgacggggtt ccggggcgac ggtaccatcg cgcccattca gtcgggaggt
      301 gggaatgcgt gactcgagga cgatgacggc cgctctggcc gtcatgatgg ctgcggctgc
      361 cctcttcgca tgcgcgggcg gtggggatgc caaggacaaa caaggggatg cggtcccggc
      421 gggggacgct tccgccacgc ctcctggcca ggatcacgcc aaccccgccc cgccggccgc
      481 cgccgagccg gagcactacg acccggcttc cgcgagcggg gcgatcaagg gggtcccggc
      541 gccgaccaac accgccacca tcgaggcggt ggcgaactac ttcgcggagg ttcccggctt
      601 cgacctcgcg ccgctcggcg agcgcggccg cgagaagttc ctgcaccgcg tcaacagcga
      661 gctctgcacg tgcgggtgca agaacgagac gatcgcctgg tgcctggtca acgatccgaa
      721 gtgccccatg gtgaagggac tggcgcaggc ggtctacgac gaggtcaaga aaggttcctg
      781 atcgcgcccc gagcgcgccg acgacgggag gagcatgaag gtctacgaca tctcggacat
      841 ccgcaacgtc ggcctcatcg gacacggctc ttccggcaag acctcgctgg cgtccgcgtt
      901 gctcttcgcc tccggcgccg tcaaccggct cggtaaggtg gaccagggga cgacggtcac
      961 cgaccacgac gaggaggaga tcgcccgcaa ggtctccatc tcctccgccc tctgccacgc
     1021 cgagtggcgc aagaccaaga tcaacctcat cgacacgccc ggttacggcg ccttcatcgc
     1081 cgacgcccgc gcctgcctgc gggtcgccga cggcgccgtg ctcctggtgg acggcgtctc
     1141 cggggtcgag gtgcagaccg aggaagcgtg gggcttcgcg caggagttca acctgccggt
     1201 gctcatcgcc gtcagccgcc tcgaccgcga gaacgcgtcg ctggagcggg ccatggcctc
     1261 gatcaccgag gccttcggcc gccaggcggt gccgatccag gtcccgatgg ggagcgagcg
     1321 cgccttcaag ggggtcatcg acctcgtcag cctcaagggg gccctctatg gcgacgacga
     1381 gagcggcaag ttccaggtcc tcgacgcgcc cagcccgctg ccgccggaga tcaaggcggc
     1441 ccgcgagcgc ctcatggagg cggtcgccga ggtggacgac gcgctgatgg agaagttctt
     1501 ctccgccggc gagctgagcc aggaggacct ggtcgccggt ctgacgcgcg gcatccgcgc
     1561 gcgacggatc ttcccggtcc tgtgcaccgc cggggcgcac aacatcgcga cccacggcat
     1621 gctcgacgcc atcgtgtcgt acctccccca ccccggcgag cgcggcccgt tcgcgggcga
     1681 gaacccgaag gatcattccg ccctcgagcg gaagggagcg acggacgagc cgtactcggc
     1741 gtacgtcttc aagaccatcg ccgacccgta ctccggcaag atctcgatgt tccgcgtcta
     1801 ttccggagtg ctcaagtcgg actccagcat ccacaacgcc agccgcgatc tcgccgagcg
     1861 cttcggctcg atcgcctcga tgcaggggaa ggaaatgcag ccggtgccgg aggtgcggtc
     1921 cggcgacatc gccgccgtcg ccaagctgaa ggagaccgcc accggcgaca ccctcgccga
     1981 gaaggcgcac ccggtcctct acccgaagct ggtcttcccc gagccgtcga tctcgttcgc
     2041 gctcaacccg aagtcgcgcg ccgacgagga caagatctcc caggtgctgc accgcgtcgt
     2101 cgaggaggac ccgaccatcc agtaccgccg ggatccgcag acgcacgagc tgatcctctc
     2161 cgggaacagc gacctgcacg tcgaggtggt gctcgccaag atgaagaaga agttcggcct
     2221 ggaggcggtg ctgcaccccc cgaaggtccc ctacctcgag acgatcaaga agaaggcgga
     2281 ggcacagggg cgccacaaga agcagaccgg ggggcacggc cagttcggcg actgctggat
     2341 caagctcgag cccctgcagc gcggcggtgg cttcgagttc gtggacgaca tcttcggcgg
     2401 ctccatcccg aagaacttca tcccctcggt cgagaagggg atccaggagg cgcgcagcca
     2461 cggctatctc gcgggatacc cggtcgtcga cttccgcgtc gtcctcttcg acggctcgta
     2521 ccacacggtc gactcctccg acatcgcctt caagatcgcc ggatccatcg cgttcaaggc
     2581 ggcgatggcg gacgccggcg cgaccctcct cgagccggtg atgaacgtcg agatcgtcgc
     2641 ccccgagcag ttcatggggg acctgatggg cgatctcaac tcccgtcgcg gccacgtgca
     2701 ggggatgtcg acccaggggc gctcgcaggt gatcaaggcg tcggtgccgc tcgtcgagat
     2761 gctgtcgtac gcgtccatcc tgaagtcgat caccggcggg cgcggctcgt accggatgac
     2821 catgcgcggc tacgaggagg tcccggccca tcagcaggcg aagatcatcg ccgcccacaa
     2881 ggcccagaag gagaaggagg cggcagccca ttagccaccc ggccggcccg gatcaccgcc
     2941 gcacgccggc cgcaagggtc ctgctcctcg gcgccgagga gaatccggcg ctgccgatcc
     3001 tcgcctcgct gtcccacgcc gggatcgcgg tcgacgtcct gagccatcgc aggaccgccc
     3061 tcggcttctt ctcccggcac gccgccgccc gcggcctctc ccccgcgccg aaggacgagg
     3121 cggcctatct cgccttcgtc ctcgcgcgcg tccgggccgt ggcgtacgac gtcgtcttcg
     3181 cggtcggcga agacccgacc tcgttcctgg cgaagcacca ggaggcgatc cgcccgcacg
     3241 cccgcgtgcc gctgcccccg ctcgagcggt tcatggtctg ccgtgacaag tcgctgacca
     3301 tgcgggaggc gcgacgtctc gggatcgcga cgccgcagac ccacgatccc gacgcggagc
     3361 cgctcgacgc gttcgcgcgc cgggtcggct tcccggccgt cgtcaagccg aacgtcagcg
     3421 acggcgcgcg cggcatctcc tacccgggcg acggcccggc gctggcccgg gccctcgcgt
     3481 ccacggaaaa ggagtacggc gcctgccacg tgcaggagta tgtcccgcag accggcacgc
     3541 agttcaaggc ggaggtgctg ctcgacggag cgtcgcggcc gcgcgcctgg tgcgtctatt
     3601 cgaagctgcg ctactatccg ccttcgggcg ggtcgagcac gctcaaccgc accgtggacc
     3661 ggcccgacat cctcgacgcg gcggcgcgca tgctccaggg gatcggctgg accggcctcg
     3721 gcgactgcga cttcatcgaa gacccgcgcg acggagtccc cagactgatg gagatcaacc
     3781 cccgcttcac ccgcagcatc cggatctgcg tgcgcgccgg ggtggatttc ccgctgctgc
     3841 tctaccggct ggcggtcggc gccggagcga tccccgagtc acccggctcc taccgcgttg
     3901 gcgtcatgca gcgctacctg ccggccgacg tcgcctggtt cctgcgatcg ccggaccgct
     3961 tccgggccag gccggggttc ttcgccacct tcttcaccgg cgcggcggac gagatctttt
     4021 ccctcgcgga cccgggtccg gccctggcgt acctcctcgg agcggcctcg gatctcctcg
     4081 acccggaggc acgacggcac cggctgcgcc cgaaagccgg cgcctccccg accggcccgt
     4141 cctccggggc cgcggcggga tgatgctgac ctttctggcc gtcgtcgtgg ccctgctgct
     4201 tctcggcgcc gcctcgttcg tgctctccgt ccacgcccag gagcaccgcc ccgatcgcat
     4261 ccctgccctc ctgtaccacc ggctcatctc caaggagcgg tatgaccggg gtgagatccg
     4321 gagcgaggag cgctcctacg tcgtgttcga cgcgtttttc gccgcccaga tggaggcgct
     4381 gcagcgggcc ggttacacga ccctgcacct cgacgactat ctgaagatcc ggcgcggtga
     4441 gatggcgcgc ccggagcggc ccatcctcgt caccttcgac gacggcttcc ggagcaacta
     4501 cctctacgcc ttcccggtcc tgcggcgact cggcatgaag gccaccatct tcatgacgcc
     4561 ggatcgatcc tcccggaact tcgagaagaa cgccccgctc gacggacccc tgagcgatga
     4621 cgagctgcgc gagatgagcg ccgccggcgt cgccatcgag tcgcacggga tgacgcaccg
     4681 ctacctcacc gacctcgacg accacaccat ccgctgggag ctcgccgagt cacgccgcgt
     4741 cctcgaagcg acgctcggcc gaccggtgcg cttcctcgcc atccccagcg gcgcctacaa
     4801 cggcaccatc cgccggctgg cgcaggacac cggatacgca gcggtcttca gcatgggcaa
     4861 ggggacgatg aacatgaaga gcgatctcct cgccctgaag cgcctggtcg tcggccgcga
     4921 cctcgacgcc gcagggctgc tctctctcct cgagcccggc aatatctttc tgctgcgcct
     4981 cacgagcacc gtgcaggtga cgttcatgcg tgtgttcggc gtccataccc tggatcggct
     5041 gcgggccttg atcctgaagt tgcccttcgg ggcggcgctc atccgccgtc ctgcggtgct
     5101 gctggcggcc ggcctctcga ccctcgccgt tctcggagcc ctgctcgtgt cgctcctctg
     5161 gcgttcgtga tcggggacgc gggacgatcg tgagccgcag cgcgggacgc cggggatgac
     5221 cgcctccctg gttctcttct ggggcgcggc cgccgccctc gcggccggac acctgctcgt
     5281 tccgctcacc ctgcgcgccc tgcggccatg gctgcgccgt cccctggaga agcggccgat
     5341 cgagccgtcc gtcagcgtcg tcatcagcgc ctcgaacgag gagcggcaca tcaccgcgaa
     5401 gatcgaggac tgcctgcgct tcgactaccc gtcgtc
//
LOCUS       DASRIQ010000511        12980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_972161, whole genome shotgun sequence.
ACCESSION   DASRIQ010000511 DASRIQ010000000
VERSION     DASRIQ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12980
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972161"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFD06_12460"
     CDS             complement(<1..876)
                     /locus_tag="VFD06_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN04386.1"
                     /translation="MSCDTARRSILLMLYGEIDGRERFKMETHLSGCGACRDAQAEER
                     RLFAMLQLESAAEPSDDLLDRCRDGLARALDAEAPPAPAPWTARLLEMWRQARLSPAY
                     GLAMLMVGFLAGAMTLRSAASGPQPLAGAAPEDASDRVASGPARPDSPPVNSVRTLEP
                     AAADGEVRVGYDTVQRGSLEGTADDPAIRALLVTTLRDSLNSGLRLEAIDALRAHAGQ
                     ADVRQAFLDVLRGDENAGARLRALDALVARAPEDSQVRGAIVDAMQQDANPGVRVRAI
                     ETLSRGRDPRLLPVME"
     gene            complement(878..1510)
                     /locus_tag="VFD06_12465"
     CDS             complement(878..1510)
                     /locus_tag="VFD06_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN04387.1"
                     /translation="MPPPDEATLISLAQAGDRAAFETLVRMHDRSVLRLALRIVKTED
                     EARDIYQESFLKAYRSLGQFRGDASFRTWLLRIVTTLCLDHLRRAAARPDAEPLEPST
                     GDEAGAGLEPPDGHPDRDPERALQRREIRRRIAAALTGLGTRERVVFELRHYEGMRLR
                     PIADLLGTTEDTVKNCLFRAHQHLRSALGDLGGAAPGMLRSSGAPAPAEV"
     gene            complement(1609..3126)
                     /locus_tag="VFD06_12470"
     CDS             complement(1609..3126)
                     /locus_tag="VFD06_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZN04388.1"
                     /translation="MNRAIVTLLAVSALSAAGILEAKPLRPAPTSAPLPIRLESSRGF
                     IVGSSPVSLTVADFNGDHHLDLAAACLGSWDVTLLMGDGTGRFTWTGSLVTAPYPSMT
                     AAGDVNADQKTDLMVIHLENGTVEVFLGDGVGGFYDPQQFSVGSNPAAVAVTDIEGDG
                     VADLVFASLEADRIAILPGTGSGGFGASRSFISGDGPVAVVARDFSGDGRIDLLSADA
                     LGDGVSILRGETNGFISVRKFPAGDRPTWMVPGDFDGDGDLDLAVSNALSNDVTILLG
                     DGIGRFGPPLSLPAGGAPSMIAAGDLDDDGDLDLATANRFSNTLSVFTGDGAGGFGPP
                     RSVAIGTSPIAIVSADFNEDGKDDLATALYETDMVMVTLSAGDADRDGFPDEADNCPA
                     TANPGQEDADGDGPGDACDNCPPLANADQGDADGNGTGDVCDALTRFLSRTQDLEEIR
                     AALIAIESQLMLLTVSNGRQQEQVEALQRDLQRVQERLQALEKIPLIRNQLKKSG"
     gene            complement(3198..5099)
                     /locus_tag="VFD06_12475"
     CDS             complement(3198..5099)
                     /locus_tag="VFD06_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04389.1"
                     /translation="MGRREWVAVVACVLLLAVFATLSWSAAATKSPVYDEPLHTVGGY
                     LIRNEGDFRVDPDHPSLWLRWASVLNGPGSLRPDHASPEWDRMFTNYWSCYLFSADTL
                     YRSPGVDPDRVVARARVMMLVAAVALGALLAFWAWHLAGPIAAVAALALFALDPALIG
                     HASLVKNDVAVAFVTLLLAFSVWRLGERLTVPRAVLTAAAFAAGFGVKYSALLFLPLL
                     VLLLIVRAVLSSDWPSVRGPLARRGHRLIAAGAVLVLCCTAAWGSIWATGGFRFAPTP
                     ESDRSLDIDALFRRMEKNHQEVARFVAADRGATAIAAAAPPWKPGPLLRAVRAAEEHR
                     LLPQAWLYGIVYTAEGMQRREGFLLGERRLTGWWYYLPVAFLFKAPIATLTSLALAAA
                     AFFPVLGRTHRPTSARRLWTAAGLVLPPLVFGLAALASKVSLGLRHAFVLYPFLFLGA
                     GVVAAECHRRWRRPVAALLLLLLAGLAVETLPAWPDEIAFFNLPSRPSRLALLGDSNF
                     DWGQDLPRLAAWRRAHPEEPLYLAYFGQADPAHYGIDARSLPTSRRDEPGTTLPSPGE
                     RATLAISATHLQGIYVKDPWLQGFYAAARWQRPKGILGTIYLYDVGLSADGSPRAASE
                     NLTPNPSEE"
     gene            complement(5224..6804)
                     /locus_tag="VFD06_12480"
     CDS             complement(5224..6804)
                     /locus_tag="VFD06_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN04390.1"
                     /translation="MSNPVAPKVTYATLAAGQTPEFTRAFDEALESVRKNLGRTWPMY
                     IGGKEVQAKETFGDTSPSDTRVVLGHFASGTREDARNAIAAARKAYEGTWRRLGWKER
                     VAHVRRLAGVIEKRRFEIAAVLSLEVGKNRLEAMGDAQETADLMSYYCDQVEKHQGYE
                     QPMETLSDREQNRDVLRPYGVWVIIAPFNFPMALAGGPSSGALLGGNTVVFKPATDTP
                     WSGALLSDCAREAGLPDGVFNFVTGPGSTVGEELVTNPDVDGILFTGSRDVGFSIYRK
                     FATRYPKPCITEMGGKNPAIVTATADLDEASEGVLKSAFGLQGQKCSACSRVYVDKRV
                     AKSFIEMLVDKTKKLAIGDPSKKEHWLGPVINRSSYEKFAGFVEMGKKDGRILSGGAQ
                     LKEEPHAHGWFVQPTILGGLPKDHHLSQEEMFLPITAVWEVASLDEALDLANRSEFGL
                     TAGLFSKDDGEIQKFFDRIEAGVVYVNRRAGATTGAWPGINPFGGWKGSGSSGKSSGG
                     PYYVEQFMREQSRTLIRG"
     gene            complement(6874..8262)
                     /locus_tag="VFD06_12485"
     CDS             complement(6874..8262)
                     /locus_tag="VFD06_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04391.1"
                     /translation="MTPPRSDPAGIGVAICATALVALCAGDLASVAGPRSRPAEVEAA
                     RRKAAAGEGPVLAGAALREITPDVGGDAAVVPLAGFGEGRLASGVRDPIFVRALVLES
                     SGAAFGMLTFDLFALGRDDAERIRREVRGRLQGPPLAGLLVVATGTRFGPDVQGVFSG
                     PGGGVDPAWKTHLVEEAAAAVEEAWRLRKPARLSFATARLPRLLEDGRRPLRLDDQAW
                     LLRVENADGKLGIAAVAQFSALPETLARGERAISADWPGGAVAAFEAALGGVGMILPG
                     AQGGRMLPAREPAGPEEYGAAVARPLVVAWSGRQEGAAPMPADVTRGKIALRSRTLAI
                     PSANGGERTTEIAFLRLASGGEPVLDLACVPGIPFPELVNGGIESPQDRGSDLQGAAP
                     EPNLRSLMAAPIRMVVGGCGDDLGEIIPASEWDARTPFAYGLESAPPGEERSPGPQTA
                     PLLWRAFADLLR"
     gene            complement(8259..9860)
                     /locus_tag="VFD06_12490"
     CDS             complement(8259..9860)
                     /locus_tag="VFD06_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN04392.1"
                     /translation="MRRSARWVAAAGAVLLSAAVPAAAGGDPAADRSNVYQESVRVVD
                     RPWERIGVTVTVTDRDGQPVRGLGCDAFRLQENGAPVDLAECGPEEGRAERPLSVAVL
                     LDLSQSMGSQIKKVEEAARALLQQLRPGDEILVAKFSDQVTVLQPFTGEEGELARGLK
                     SIGRARGGTALFRSIESTLKDVRTRRGRKVILVISDGQDNALERAGQVTQSLYMQDLL
                     RLCLRTQTVVYGIRPGMPSSWQSFEEFVQATGGRMLYTGGDLERLFARLGEEFRSQYY
                     LAWDIDPKNHATGWRRLRVSLARTDLVVHAIDGYFTPRDRLESLLHDLDDRDDEMRQD
                     AAYDLGFVDDPRARLALRSALDDRDPGVRRLALEGLGRLREELAIEGMIAALGDPSPE
                     VREAAAAALGAFGAAAIEPLCDVVAAGAGKRRPKQRVLGAAAVLGSAGDDRAVEPLTA
                     LLRSPAPEARRMAAHALRALGLASGLAPLREALLDAAPEVRQAAAEGLAALAGAAARP
                     VLEARLAVETDAGVRAALLHLLQGP"
     gene            9923..10840
                     /locus_tag="VFD06_12495"
     CDS             9923..10840
                     /locus_tag="VFD06_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HZN04393.1"
                     /translation="MQHLVRIAAVVVLFGFGCGAGDEAAPLAASPASKPAAGRTPAAS
                     PAPVAETPWERLEPGLEIAAFRSRLAAAGDRMVNVLRIDPTRFRLRLLNASSEPGGRP
                     RTVRDWSERYHLTAAINASMYQADMRSSVSLMLTRGHVNNPRVSKDKAVLAFDPLTEG
                     LPAVQIIDRECQDFGTLREQYGTLVQSIRMVSCDGRNVWEPQQGAWSTAAIGADKEGR
                     LLFIHARAPHTTHDLIEVLLELPLQLKNAMYVEGGSEAQMFVQSGGRKLEFAGSIEDP
                     PEAVTAARLVWPIPNAVGIEPIPPQPARR"
     gene            complement(10822..12126)
                     /locus_tag="VFD06_12500"
     CDS             complement(10822..12126)
                     /locus_tag="VFD06_12500"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN04394.1"
                     /translation="MSWNLPERPLAAGAGLPAVAVLVSAAAAGLAVASCGGQPPLEAD
                     LILRHAIIETGAGQVEALAVREGHVLATGDDAAVGRHRGRKTIELDLKGGRVAPGFTV
                     PVADPLLVGERLLNEARGGELYLDLADAESVEDIVQRARARARAAGPGEWIFGRDWDE
                     TRWVAPRLPDKRLISDIVAYNPVFLLRRGGEIAWVNKQALDRAGLAGDGLLHGPSVAA
                     ALRRAPPVPVEVRQKAILAALDQAVSMGVTEVRAIASGARLGVRDPEASEEAVLGAWR
                     ALARSGRLPVGVSLLVPAPGAAAEAVLVHRPATGTVSADLLDAAILLDPPSEDAAAWC
                     GRAKQAGVSCLLAAGGDQAVLQAAEAACRGAGLERVETLAAGAASPPDRFGEPIRTAN
                     PRALTPGERADFVLLGPARDGEQSPRILSTWVGGREVYRRAG"
     gene            complement(12128..>12980)
                     /gene="corA"
                     /locus_tag="VFD06_12505"
     CDS             complement(12128..>12980)
                     /gene="corA"
                     /locus_tag="VFD06_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZN04395.1"
                     /translation="DEIAILTGVFRFHPLAIEDCLNRRHSPKVDDFKDYLFILSHGVH
                     PESSVREFKTRPLSLFVGRSYIVTFHRDKSRSVEYAMESARRNPRVMAEGPDSVLHAI
                     LDYQVDQYLPVLEVFEKKIEDVEGRVFTAPTEEVLTDVLAFKKALMRLRRIAGHQRDI
                     LMRLVRREFPMIDEKAIYGLRDVSDHLVRITDLADTYRDVVAGALEAHLSIVSNRTNE
                     IMRVLTVIATLFIPLTFVVGVYGMNFDHMPELHWRYGYLAVWVLMLGVAGGMYWYFRR
                     RGIFGGR"
BASE COUNT         1926 a         4679 c         4604 g         1771 t
ORIGIN      
        1 ctccatcacg gggagcaggc gcggatcgcg cccgcgcgac agcgtctcga tggcgcgcac
       61 ccgcactccc ggattggcgt cctgctgcat cgcatccacg atggcgccgc gcacctggga
      121 atcctccggg gcgcgcgcca ccagcgcgtc gagcgccctg agccgggcgc cggcgttctc
      181 gtcgccgcgc agcacgtcga ggaacgcctg ccggacgtcc gcctggccgg cgtgggctcg
      241 cagcgcgtcg atcgcctcga gccgcagccc cgagttcaga ctgtcgcgca gcgtggtcac
      301 gaggagcgcc cggatcgccg ggtcgtcggc cgtcccttcg agcgagcccc gctgcacggt
      361 gtcgtagccg acccgtactt cgccgtcggc cgcggccggc tcgagggtgc ggacgctgtt
      421 caccggcggg ctgtccggcc gggccgggcc cgaggcgaca cggtcggagg cgtcctcggg
      481 cgcggcgccg gccagaggct gaggcccgga ggcggcggac cgaagcgtca tcgcccccgc
      541 gaggaacccg accatcagca tcgcgagccc gtaggccggg gacaggcgtg cctgccgcca
      601 catctccagc agccgcgcgg tccagggggc gggagcggga ggcgcctcgg cgtcgagggc
      661 ccgggccagg ccgtcgcggc agcggtcgag caggtcgtcg gaaggctcgg ccgcggactc
      721 gagctgcagc atcgcgaaga gccgtcgttc ctccgcctgg gcgtcgcggc aggcgccgca
      781 gccggacagg tgcgtttcca tcttgaagcg ctcgcggccg tcgatctcgc cgtagagcat
      841 cagcagaatc gaacgccggg cggtgtcgca gctcatctca aacctccgcc ggggccgggg
      901 ccccggacga acgcagcatc cccggggcgg cgcccccgag gtctccgagc gccgagcgca
      961 ggtgctggtg ggcccggaac aggcagttct tcaccgtgtc ctccgtggtg ccgaggagat
     1021 cggcgatcgg ccggaggcgc atcccttcgt agtggcgcag ctcgaagacc acccgctcgc
     1081 gcgtcccgag cccggtaagc gccgcggcga tgcgccggcg gatctcccgc cgctgaagag
     1141 cccgctccgg gtcccgatca gggtgcccgt caggaggctc gagccccgcc ccggcctcgt
     1201 cgccggtcga aggctcgagc ggctcggcgt cgggacgggc cgccgcgcgg cgcaggtggt
     1261 cgaggcagag ggtcgtgacg atgcgcagga gccatgtcct gaaggacgca tccccgcgga
     1321 actggccgag gcttcgatac gccttgagga agctctcctg gtagatgtca cgggcctcgt
     1381 cctccgtctt cacgatcctc agcgccagcc tcaggacgct ccggtcgtgc atcctgacca
     1441 gggtctcgaa agcggcccgg tcaccggcct gggccaggga gatcagcgtt gcttcgtcgg
     1501 gtggcggcac gaagtgctca tccttggatc cgcttgcctg gttcccggtc gcgtcctgac
     1561 atctggatag acggacgggg ccctgaaagg taagcgttcg gaaggaaatt acccggattt
     1621 cttgagctgg ttcctgatca aggggatctt ttcgagggcc tggagccgct cctggacccg
     1681 ctggaggtcg cgctgcaggg cctccacctg ctcctgctgc cggccgttgg agacggtcag
     1741 gagcatgagc tggctctcga tcgcgatgag agccgcccgg atctcctcca ggtcctgggt
     1801 ccggctcagg aaccgggtca gggcgtcaca gacgtccccg gtcccgttgc cgtcggcatc
     1861 cccctgatcg gcgttggcca gaggggggca gttgtcgcag gcgtctccgg gcccgtcccc
     1921 gtcggcatcc tcctgcccgg ggttcgcggt ggccgggcag ttgtccgcct cgtccgggaa
     1981 cccgtcgcgg tcggcatcac cggcgctgag ggtgaccatg accatgtcgg tctcgtacag
     2041 ggcggtcgcg aggtcgtcct tcccgtcctc gttgaaatcg gcggagacga tggcgatcgg
     2101 cgaggtgccg atggcgaccg accgcggcgg cccgaacccg cccgcgccat ccccggtaaa
     2161 gaccgagagc gtgttggaaa accggttcgc ggtggcgaga tcgaggtcgc cgtcatcatc
     2221 caggtcgccg gccgcgatca tcgatggcgc accgccggcg ggaagggaga ggggggggcc
     2281 gaagcggccg atgccgtccc cgaggaggat cgtgacgtcg ttcgagaggg cgttgctgac
     2341 ggcgaggtcg aggtcgccgt cgccgtcgaa atcccccggc accatccagg tcgggcggtc
     2401 gccggccggg aacttccgga cgctgatgaa cccgttcgtc tcgccgcgga ggatcgagac
     2461 gccatcgccg agggcgtcgg cactcaggag atcgatgcgg ccgtcgccgc tgaaatccct
     2521 cgcgaccacc gcgacgggac cgtccccgct gatgaagctg cgcgaggccc cgaacccccc
     2581 ggagccggtc ccggggagga tcgcgatccg gtccgcttcg aggctggcga agaccagatc
     2641 ggccacgcca tcaccctcga tgtcggtcac cgccaccgca gccggattgc tgccgacgga
     2701 gaactgctgc gggtcgtaga agccgccgac tccgtccccc aggaagacct cgaccgtgcc
     2761 gttttcgagg tggatgacca tcagatcggt cttctggtcg gcgttcacgt cccctgccgc
     2821 ggtcatggag ggataagggg cggtgacgag cgagccggtc caggtgaacc ggccagtgcc
     2881 atcccccatc aggagggtga cgtcccatga cccaaggcac gccgcggcca ggtcgaggtg
     2941 gtggtcgcca ttgaaatcgg ccacggtgag ggagaccggg ctgctcccga cgatgaatcc
     3001 gcgcgacgat tccagccgga tcgggagcgg agcggaggtc ggcgccggcc tcagcggctt
     3061 cgcctccagg atgccggcgg ccgacagagc ggacactgcg agaagcgtga cgatggcgcg
     3121 gttcatgacc ccaacctccc gggcacggcc gcacaccgtg tcatgagacc ttttatgggt
     3181 ggaccaccac gtcctgccta ctcctccgag gggtttggag tcaagttctc ggaggcggcc
     3241 cggggggacc cgtccgcgga gaggccgacg tcgtacaggt agatcgtccc gaggatccct
     3301 tttggtctct gccaccgcgc ggcggcgtag aacccctgga gccagggatc cttcacgtag
     3361 atcccctgca ggtgcgtggc gctgatggcg agcgtggccc gttcgcccgg cgacggcagc
     3421 gtcgttcccg gctcgtcccg gcgcgacgtc ggcaggctgc gggcgtcgat gccgtagtgg
     3481 gccggatcgg cctggccgaa gtaagcgagg tacagcggct cctccggatg ggcccgacgc
     3541 caggctgcga gccgcggcag gtcctgcccc cagtcgaagt tggagtcccc caggagcgcg
     3601 agccgggatg gccgtgaggg gaggttgaag aaggcgatct cgtccggcca cgccggaagc
     3661 gtctcgaccg ccaaccctgc cagcaggagc agaaggaggg ccgcgaccgg ccgccgccag
     3721 cggcgatggc actctgcggc caccaccccg gcccccagaa ataagaaagg atagaggacg
     3781 aaggcgtggc ggaggccgag gctgaccttc gaggcaagag ccgcgagacc aaagacgagc
     3841 ggcggcagga cgaggccggc ggcggtccag agccgacgtg ccgacgtggg acgatgcgtc
     3901 cgaccgagga ccgggaagaa ggcagctgcc gccagcgcca gtgaagtgag cgtggcgatc
     3961 ggggccttga acagaaaggc caccggcagg tagtaccacc agccggtgag ccggcgctcg
     4021 cccagaagaa atccctcgcg ccgctgcatc ccttcggccg tgtagacgat gccgtagagc
     4081 cacgcctgcg gcaggagcct gtgctcttcg gcggcccgca cggcgcgcag gagcgggccg
     4141 ggcttccagg gcggtgccgc ggcggcgatc gcggtggcgc cgcggtctgc ggcgacgaag
     4201 cgggccactt cctggtggtt tttctccatc cgacggaaca gcgcatcgat gtcgagcgac
     4261 cggtccgact ccggcgtggg agcgaagcga aacccgccgg tggcccagat cgatccccac
     4321 gcggcggtgc agcaaagcac gaggaccgcg ccggccgcga tcaggcggtg tccgcgtctg
     4381 gcgagggggc ctcgcacgga cggccagtcc gacgacagga ccgcgcgcac gatcagcagc
     4441 aggacgagga gcggcaggaa cagcagcgcc gaatacttga ccccgaaccc cgccgcgaac
     4501 gccgccgccg tcaggaccgc ccgcggcacc gtgagccgct cccccagccg ccagacggaa
     4561 aacgcgagga gaagcgtcac gaaggcaacc gcgacgtcgt tcttcacgag cgaagcgtgt
     4621 ccgatgagcg ccggatcgag cgcgaacagg gccagcgcgg ccacggccgc gatcggtccg
     4681 gcgagatgcc acgcccagaa cgcgagcagc gcccccagcg ccaccgcggc gacgagcatc
     4741 atgactcgcg cccgcgccac cacccgatcg gggtcgaccc cgggggagcg gtacagcgtg
     4801 tcggcggaaa agaggtagca ggaccagtag ttcgtgaaca tcctgtccca ctcggggctg
     4861 gcgtggtccg gccgcagcga acccgggccg ttcaggacgg acgcccagcg gagccacagc
     4921 gacggatgat ccggatcgac ccggaaatcc ccttcgttcc ggatcaagta cccgccgacc
     4981 gtgtggagcg gctcgtcata caccggcgac ttcgtcgccg ccgcgctcca tgagagcgtc
     5041 gcgaagaccg ccagcagcaa cacacacgcg acgacggcca cccactcgcg cctgcccatc
     5101 gagggcggag tatacggccc caccggcgtt gggcagtcag cagcggcgca ggcatctcgg
     5161 gggcccgggg cgcgggaagc cgccgcgggg gcgtgagaag cgtgtgctgt tgctgctggc
     5221 tgcttagccg cggatgagag tgcggctctg ctcgcgcatg aactgctcga cgtaatacgg
     5281 cccgccgctc gacttgccgg atgagccgct ccctttccag ccgccgaagg ggttgatgcc
     5341 tggccaggcg ccggtggtgg cgccggcgcg ccggttgacg tagacgactc cggcttcgat
     5401 ccggtcgaag aacttctgga tctcgccgtc gtccttgctg aacagcccgg cggtgaggcc
     5461 gaactcgctg cggttggcga gatcgagcgc ctcgtcgagg gacgccacct cccagaccgc
     5521 ggtgatcggc aggaacatct cctcctgcga caggtggtga tccttcggga gcccgccgag
     5581 gatggtcggc tgcacgaacc agccgtgcgc gtgcggctcc tccttgagct gcgcgccgcc
     5641 gctgaggatc ctgccgtcct ttttccccat ctccacgaag ccggcgaact tctcgtacga
     5701 cgaccggttg atcaccggcc cgagccagtg ctccttcttc gacgggtcgc cgatggcgag
     5761 cttcttcgtc ttgtccacga gcatctcgat gaagctcttc gcaacccgct tgtccacgta
     5821 gacgcgggag caggcggagc acttctggcc ctgcaggccg aacgccgact tgagcacccc
     5881 ttcgctcgcc tcgtcgaggt cggcggtggc ggtgacgatc gccgggttct tcccccccat
     5941 ctcggtgatg cacggcttcg ggtagcgcgt ggcgaacttg cggtagatgg agaaaccgac
     6001 gtcgcggctg ccggtgaaca ggatgccgtc cacatccggg ttcgtgacca gctcctcccc
     6061 caccgtcgag ccggggccgg tgacgaagtt gaagaccccg tccggcagtc ccgcctcgcg
     6121 ggcgcagtcg ctgaggagcg cgccgctcca cggcgtgtcg gtggccggct tgaagacgac
     6181 ggtgttgccg ccgaggagcg ccccgctgga ggggccgccg gcgagcgcca tcgggaagtt
     6241 gaacggcgcg atgatcaccc agactccgta cggccgcagg acgtcgcggt tctgctcccg
     6301 gtccgacagc gtctccatcg gctgctcgta accctggtgt ttctcgacct ggtcgcagta
     6361 ataggacatc aggtcggcgg tctcctgagc gtcccccatc gcctcgagcc ggttcttgcc
     6421 gacttccagg ctcagcaccg cggcgatctc gaagcggcgc ttctcgatga cgccggcgag
     6481 ccgccgcacg tgcgccaccc gctccttcca gccgaggcgg cgccaggtcc cctcgtaggc
     6541 cttccgcgcc gccgcgatgg cgttgcgcgc gtcctcgcgc gtgccgctgg cgaagtggcc
     6601 gaggaccacg cgcgtgtcgc tcgggctcgt gtccccgaac gtctccttcg cctgcacctc
     6661 cttcccgccg atgtacatcg gccaggtgcg gccgaggttc ttccggacgc tttcgagcgc
     6721 ctcgtcgaag gcgcgcgtga actccggcgt ctggccggcg gcgagggtgg cgtaggtgac
     6781 cttgggggcg acgggattcg acatcggatc tcctcaggtt tcagcggagc ggcgattgta
     6841 ccgcaggggg ccggccgcgc ggcgcgatcg cggtcagcgc aggagatccg cgaaggcgcg
     6901 ccagaggagc ggagcggtct gcggtcccgg ggagcgctcc tcgcccggag gggccgactc
     6961 cagcccgtag gcgaacggcg ttcgggcgtc ccattcggac gccgggatga tctcgccgag
     7021 atcgtcgccg cacccgccca cgaccatccg gatgggcgcc gccatcaggg agcggaggtt
     7081 cggctccggc gccgccccct gcaggtcgga gccacggtcc tgcgggctct cgatgccgcc
     7141 gttgaccagc tccgggaacg gaattccggg cacgcatgcg aggtccagga ccggctcgcc
     7201 gccggaggcg aggcgcagaa aagcgatctc ggtcgtccgc tcgccgccgt tcgcggacgg
     7261 aatggcgagg gtgcgcgatc gcagcgcgat cttgccgcgc gtcacgtcgg ccggcatcgg
     7321 ggccgccccc tcctgccgac ccgaccaggc gacgacgagc gggcgcgcca ccgccgcgcc
     7381 gtactcctcc gggccggccg gctcgcgggc cgggagcatc cgccccccct gcgcgccggg
     7441 gaggatcatt cccacgccgc cgagcgccgc ctcgaaggcg gcgacggctc cgccgggcca
     7501 gtcggcggag atcgcgcgct cgccgcgcgc cagcgtctcc ggcagcgccg agaactgcgc
     7561 cacggcggcg atcccgagct tgccgtcggc gttctcgacg cgcagcagcc acgcctggtc
     7621 gtcgaggcgc agcggccgcc gcccgtcctc gaggaggcgc ggcagccgcg ccgtcgcgaa
     7681 cgacaggcgc gccggcttgc ggaggcgcca cgcctcctcg acggcggcgg ccgcctcctc
     7741 gacgaggtgg gtcttccagg cgggatcgac cccgcctccg ggaccggaga acaccccctg
     7801 cacgtccggc ccgaagcggg tcccggtcgc gacgacgagc aacccggcga gcggcggtcc
     7861 ctgcaggcgg ccgcgcacct cgcggcggat ccgttcggcg tcgtcccgcc ccagcgcgaa
     7921 cagatcgaac gtgagcatcc cgaacgccgc gccgctgctt tcgaggacga gggcgcgcac
     7981 gaagatgggg tcgcgcaccc cggaggcgag ccggccttcg ccgaagccgg ccagggggac
     8041 cacggcggcg tctccgccga catccggagt gatctcgcgc agcgcggctc ccgcgaggac
     8101 cggcccttcg ccggctgccg ccttcctgcg cgcggcctcc acctctgcgg gccgggacct
     8161 gggaccggca accgaagcca ggtcaccggc gcacagcgcg accagcgcgg tggcgcagat
     8221 cgcgacaccg atgcccgccg gatccgaccg tggcggcgtc acgggccctg cagcagatgg
     8281 aggagcgcgg cccgcacgcc ggcatcggtt tcgaccgcca gccgcgcctc gaggaccggc
     8341 cgcgccgccg ctccggcgag ggccgccagg ccctccgccg ccgcctgccg gacttcgggg
     8401 gcggcatcga ggagggcctc gcgcagcggc gccaggccgg aggcgagtcc gagcgcccgc
     8461 agcgcatgcg ccgccatccg ccgcgcctcg ggcgctgggg aacgaagaag cgctgtgagc
     8521 ggctcgaccg cccggtcgtc gccggccgac ccgaggaccg ccgcggctcc gaggacgcgc
     8581 tgcttcggac ggcgctttcc ggcgccggcc gcgacgacgt cgcacagagg ctcgatcgcc
     8641 gcggcgccga aggcaccgag cgcggcggcc gccgcctcgc gcacctcggg cgaaggatcc
     8701 ccgagcgccg cgatcatccc ctcgatcgcc agctcctcgc gcagccgtcc cagcccctcg
     8761 agcgccaggc ggcggacgcc gggatcgcga tcgtcgaggg cggagcgcag ggccaggcgg
     8821 gcgcgcgggt cgtcgacgaa gccgaggtca taggccgcgt cctgccgcat ctcgtcgtcc
     8881 cgatcgtcga ggtcgtgcag caggctctcg agacggtcgc gcggcgtgaa gtagccgtcg
     8941 atggcgtgca ccaccagatc ggtgcgggcg agggagacgc gcagccgcct ccagccggtc
     9001 gcgtggtttt tcggatcgat gtcccaggcg aggtagtact ggctgcggaa ctcctccccc
     9061 agccgcgcga acaggcgctc gagatcgccg ccggtgtaga gcatgcggcc ccctgtcgcc
     9121 tgcacgaact cctcgaacga ctgccacgac gacggcatgc cgggacggat gccatagacg
     9181 acggtctgtg tcctcaggca caacctcaag agatcctgca tgtaaagcga ctgggtcacc
     9241 tggccggccc gctccagcgc gttgtcctgg ccgtcgctga tcacgaggat caccttgcgt
     9301 ccgcgccggg tgcggacgtc cttgagggtc gattcgatcg accggaacag ggcggtgccg
     9361 ccgcgggcgc ggccgatcga cttgagcccg cgcgccagct ccccctcttc gccggtgaac
     9421 ggctgcagga cggtgacctg gtcgctgaac ttcgcgacca ggatctcgtc cccgggacgc
     9481 agctgctgaa ggagggccct ggccgcctcc tccaccttct tgatctggct ccccatgctc
     9541 tgcgacagat ccaggaggac agcgaccgac agcggccgtt cggcgcgccc ttcctccggc
     9601 ccgcactcgg cgaggtccac cggcgcgccg ttctcctgga gccggaacgc gtcgcacccg
     9661 aggccgcgga ccggctggcc gtcccggtcg gtgacggtca cggtcacccc gatccgctcc
     9721 cagggacgat cgaccacccg cacgctctcc tggtagacgt tcgatcgatc ggccgccgga
     9781 tcccctccgg cggccgccgg gaccgcggcg gaaaggagca ccgctcccgc cgctgccacc
     9841 caccgcgcgc ttcgtcgcat acgcacattc taggatcgcg tcccgtccct tcaccgccag
     9901 tcgaatagaa tccaccgccg tgatgcagca cttggttcgg atcgcggcgg tcgtcgtcct
     9961 tttcggcttc ggatgtggtg ccggggacga agcggccccg ctcgcggcct ctccggcgtc
    10021 gaaaccggcc gccggcagga cgccggccgc ctcccccgcc ccggtggccg agactccgtg
    10081 ggagcgtctc gagccggggc tcgagatcgc cgccttccga tcgcggctcg cggcggccgg
    10141 ggatcggatg gtgaacgttc tgcgcatcga cccgacgcgg ttccgcctgc gcctgctcaa
    10201 cgcctcgtcc gagcccgggg gccggccgcg caccgtgcgg gactggtcgg agcgctatca
    10261 cctcaccgcc gccatcaacg ccagcatgta ccaggcggac atgcgctcga gcgtctccct
    10321 gatgctcacc cggggccacg tcaacaatcc ccgcgtgtcg aaggacaagg cggtgctggc
    10381 gttcgatccg ctcacggagg ggctgccggc ggtgcagatc atcgaccggg agtgccagga
    10441 tttcggaacg ctgcgcgaac agtacggcac cctcgtgcag agcatccgga tggtctcgtg
    10501 cgacggacgc aacgtgtggg agccgcagca gggggcctgg agcaccgcgg ccatcggcgc
    10561 cgacaaggag gggcggctgc tgttcatcca cgcccgggcg ccgcacacga cccacgacct
    10621 catcgaagtg ctgctcgagc tgcctcttca actgaagaac gccatgtacg tcgaaggggg
    10681 aagcgaggcg cagatgttcg tgcagtcggg cgggcggaag ctggaattcg ccggctcgat
    10741 cgaggacccg ccggaggcgg tgacggcggc ccgcctcgtc tggccgatcc cgaacgccgt
    10801 cgggatcgag ccgatccccc ctcagccggc gcggcggtag acctcccggc cgccgaccca
    10861 ggtcgacagg atccgcggcg actgctcccc gtcccgcgcg ggaccgagca gcacgaaatc
    10921 cgccctctcc cccggggtca gcgctcgcgg gttcgcggtc cggatcggct ctccgaagcg
    10981 atcgggcggc gacgcggcgc ccgccgcgag cgtttcgacc cgctcgagcc ccgcgccgcg
    11041 gcaggcggcc tcggccgcct ggagcaccgc ctggtctccg ccggccgcaa gtagacagga
    11101 cactcccgcc tgcttcgcgc gaccgcacca ggcggcggcg tcctccgacg gcgggtccag
    11161 gaggatcgcc gcatcgagga ggtccgccga cacggtcccc gtcgcgggac ggtgcacgag
    11221 gaccgcctcg gccgccgctc caggcgccgg cacgagcagc gacaccccca ccggcagccg
    11281 tccggagcgg gccaacgcac gccaggcgcc gagcaccgcc tcctcgctcg cttccgggtc
    11341 gcgcaccccg agacgggcgc ccgaggcgat cgcccgcacc tccgtaacgc ccatcgagac
    11401 ggcctggtcc agcgcggcca ggatcgcctt ctggcgcacc tccaccggga cggggggcgc
    11461 gcgtcgcagc gcggcggcca ccgacgggcc gtggagcagg ccgtcccccg ccaggccggc
    11521 ccggtcgagc gcctgcttgt tgacccacgc gatctcgccg ccgcggcgca ggaggaagac
    11581 cgggttgtac gccacgatgt cgctgatgag ccgcttgtcg gggaggcgcg gcgccaccca
    11641 gcgggtctcg tcccagtccc ggccgaagat ccactccccc ggcccggcgg cgcgcgcccg
    11701 cgcccgcgcc cgctgcacga tgtcctccac gctctcggcg tcggcgagat cgaggtacag
    11761 ctccccgccc cgggcttcgt tgagcagccg ctcgccgacg aggagcggat cggcgaccgg
    11821 gacggtgaac cccggcgcca cccgcccccc cttgagatcg agttcgatcg tcttccggcc
    11881 gcgatggcga ccgaccgcgg cgtcgtcgcc ggtcgcgagc acgtgcccct cccgcaccgc
    11941 gagcgcttcg acctgtccgg cgccggtctc gatgatggcg tgcctcagga tcaaatccgc
    12001 ttcgagcgga ggctggccgc cgcaggaggc cacggcgagg ccggccgccg cggcggagac
    12061 aaggacggcc acggcgggaa gaccggcccc ggccgccagc gggcgctcgg gaaggttcca
    12121 ggacacctca gcggccgccg aagatgccgc ggcgccggaa gtaccagtac atcccgccgg
    12181 cgacgcccag catgaggacc cagacggcga gatagccgta gcgccagtgc agctccggca
    12241 tgtgatcgaa gttcatgcca tagacgccga cgacgaaggt cagcgggatg aacagggtgg
    12301 cgatgaccgt caggacccgc atgatctcgt tggtgcggtt ggagacgatc gacaggtgcg
    12361 cctcgagcgc cccggcgacg acgtcgcggt aggtgtcggc caggtcggtg atccgcacga
    12421 ggtgatccga cacgtcgcgc aacccgtaga tcgccttctc gtcgatcatc gggaactccc
    12481 gccgcaccag tcgcatcagg atgtcgcgct ggtgacccgc gatgcgccgc agccgcatca
    12541 gcgccttctt gaacgccagg acgtccgtca gcacctcttc cgtcggcgcg gtgaagacgc
    12601 gcccctccac gtcctcgatc ttcttctcga agacctccag gactggcagg tactggtcca
    12661 cctggtagtc gaggatggcg tggaggacgg aatccggccc ctccgccatc acccgcgggt
    12721 tgcggcgcgc gctctccatg gcgtactcga cgctccgcga cttgtcgcgg tggaaggtca
    12781 cgatgtagga gcgcccgacg aacaggctga gcggccgggt cttgaactcg cgcaccgacg
    12841 actcggggtg cacgccgtgc gacaggatga acaggtagtc cttgaagtca tccactttcg
    12901 ggctgtgccg gcggttgagg cagtcctcga tcgccagcgg gtggaagcgg aacacccccg
    12961 tgaggatggc gatctcgtcc
//
LOCUS       DASRIQ010000512         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2022257, whole genome shotgun sequence.
ACCESSION   DASRIQ010000512 DASRIQ010000000
VERSION     DASRIQ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022257"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFD06_12510"
     CDS             complement(<1..314)
                     /locus_tag="VFD06_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04396.1"
                     /translation="MRDTGALRAPSDGRGAVRTVSGRPLAADLLALRELQTSLGLEES
                     DLGVIRVGTNAIVEILAIDCGLALVEATATRPEIRFGWCQGRAMPQAEVEALLRALER
                     DL"
     gene            368..1036
                     /gene="thiE"
                     /locus_tag="VFD06_12515"
     CDS             368..1036
                     /gene="thiE"
                     /locus_tag="VFD06_12515"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZN04397.1"
                     /translation="MTSGWPRYHLYVLTDVTLVPGRTHTDIVRAALAGGADAVQIRDK
                     ATTAQNLGRAVAECQPLARKFGAALLVNDRVDVALLAGADGAHVGPEDLPARAARRLL
                     KRPALLGVSAGTLEEAKKAVRDGADYLGVGPIFGSRTKPDAGPPLGLEPLEAIVRAVQ
                     VPVVAIGGIDHENVAAVAATGCAGAAVIAAVVTAPDMAAAARAIKTRFLEGRRAITGG
                     AGPR"
     gene            1081..1890
                     /gene="thiD"
                     /locus_tag="VFD06_12520"
     CDS             1081..1890
                     /gene="thiD"
                     /locus_tag="VFD06_12520"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZN04398.1"
                     /translation="MTVALTIAGSDSGGGAGIQADLKTFAAFGVFGTSAITAVTAQNT
                     VAVRAVQEIDPGVVAAQIDAVAEDFPIAAVKTGMLSNAAIVEVVAAAIERRRLPHLVV
                     DPVMVSKSGAVLLRKEAVEALRLRLLPLAEMVTPNLQEAERLSGVTISDEATAREAAR
                     RIAAHGARTVLVKGGHGPGAEAIDLFHDGRIFESFRGPRLETRNTHGTGCTLSAAIAA
                     LLALGSSPLEAAFRSKAWLHDAILAAPGLGSGTGPVDHFVAGIPAKTPSTT"
     gene            complement(1895..2449)
                     /locus_tag="VFD06_12525"
     CDS             complement(1895..2449)
                     /locus_tag="VFD06_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04399.1"
                     /translation="MHRHALSCAALLVLAPMAVFAGAPPGAAPAAPAAGAALDGKAAL
                     ERLKALAGTWEGTIVGSEKAPAKSIFSVTAGGTAVVEKMFPGTPHEMVTVYFLEGSAL
                     RATHYCTAGNQPHMRFDAKASTADDLRFAFDGGTGFDPKKDLHVHSARFTFSPDGALT
                     EEWEFHNEGGKNGTHAMTLTRAAS"
     gene            complement(2553..>3288)
                     /locus_tag="VFD06_12530"
     CDS             complement(2553..>3288)
                     /locus_tag="VFD06_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN04400.1"
                     /translation="EDDGLRIALLADLHTGSAWNDAVKLREIVRRTNAAEPHLVLLLG
                     DYVVHGVLGGHEVPPETVASLLGDLRAPLGVYAVLGNHDYARNGPRIRGALRRFGITV
                     LEDESVRLGGGSGPPGADAPFWITGVSDLLRAPHDLHAALAMVTDDAPILVFTHNPDL
                     FPEVPARVALTLAGHTHGGQVRLPFFGRPIVPSLHGQRFAAGHVIEEGRHLYVATGLG
                     TSIIPVRFLVPPEIVVLELHRAPERP"
BASE COUNT          460 a         1149 c         1229 g          450 t
ORIGIN      
        1 ttcagatcgc gctcgagggc gcgcagcagc gcttccacct ccgcctgcgg catcgcgcgc
       61 ccctggcacc agccgaagcg aatctcgggg cgggtggcgg tcgcttcgac gagcgcgagg
      121 ccgcagtcga tggcgaggat ctcgacgatg gcgttggtgc cgacccggat gacgccgagg
      181 tcgctttcct cgaggccgag actcgtctgc agctcgcgca gcgcgagcag gtcggcggcc
      241 agcggccggc cggacaccgt ccgcacggcg ccgcggccgt ccgaaggggc gcgcagcgcc
      301 cccgtgtccc tcatcgtgag gtcctcgaac ccggtgctag aatggccgcc atccctccgg
      361 agaccgcatg acgtccggct ggccccgcta ccatctctac gtgctcaccg acgtgacgct
      421 cgtcccgggc cgcacgcaca ccgacatcgt gcgcgccgcg ctcgcgggag gcgccgacgc
      481 ggtgcagatc cgcgacaagg cgacgacagc ccagaactta ggccgggcgg tggccgaatg
      541 ccagccgctg gcgcggaaat tcggggcggc gctcctggtc aacgaccggg tggacgtggc
      601 tcttctggcg ggggcggacg gggcccacgt cggccccgag gacctgccgg cgcgggcggc
      661 gcggcgcctt ctgaagcggc cggccctcct cggggtgtcc gccggcacgc tcgaggaggc
      721 gaagaaggcg gtgcgcgacg gcgccgatta cctcggcgtc ggcccgatct tcgggagccg
      781 gacgaagccg gacgccggcc ctcccctcgg gctcgagccg ctcgaggcga tcgtgcgcgc
      841 ggtgcaggtg ccggtcgtgg cgatcggcgg catcgatcac gaaaacgtgg ccgcggtcgc
      901 cgccacgggc tgcgctgggg cggcggtcat cgcggccgtg gtgaccgccc cagacatggc
      961 ggccgccgcc cgcgcgatca aaacccgctt cctcgagggc cgccgcgcaa tcaccggcgg
     1021 ggcgggcccg cgatgagccc ctccatggtc cagggcgtcc ccggcgcgcg gagcggcgcc
     1081 atgaccgtgg cgttgacgat cgccggatcg gactccggcg gcggcgccgg catccaggcc
     1141 gacctcaaga cgttcgccgc gttcggcgtc ttcgggacgt ccgcgatcac cgccgtcacg
     1201 gcgcagaaca ccgtcgcggt gagggcggtg caggagatcg atccgggcgt ggtggcggcg
     1261 cagatcgacg ccgtcgccga ggattttccg atcgccgcgg tcaagaccgg gatgctctcg
     1321 aacgccgcga tcgtcgaggt ggtcgcggcg gccatcgagc gccgcaggct gccgcacctc
     1381 gtcgtcgatc cggtgatggt gagcaagagc ggcgcggtgc tcctccgcaa ggaggccgtg
     1441 gaggcgctgc ggctgcggct cctgcccctg gccgagatgg tgactccgaa cctgcaggag
     1501 gcggagcgtc tctccggcgt gacgatctcc gacgaagcga cggcgcgtga ggcggcccgg
     1561 cgcatcgcgg cccacggcgc tcggacggtc ctcgtcaagg gcgggcacgg gcccggcgcg
     1621 gaggcgatcg atctcttcca cgatggccga atcttcgaga gcttccgcgg cccgcggctc
     1681 gagacgcgga acacccacgg caccggctgc accctgtcgg cggcgatcgc cgcgctcctg
     1741 gccctcggca gcagtccgct cgaggcggcg tttcggtcca aggcatggct gcacgacgcg
     1801 atcctcgccg cccccggcct tggaagcggc accggaccgg tcgatcactt cgtcgccggg
     1861 atccccgcga agaccccctc gaccacctga agcctcagga cgccgcgcgc gtcagcgtca
     1921 tcgcgtgggt gccgtttttc ccgccttcgt tgtggaactc ccactcctcg gtcagcgccc
     1981 catccggcga gaacgtgaac cgggccgagt ggacgtgcag gtccttcttg gggtcgaagc
     2041 cggtgcctcc gtcgaaggcg aagcgcagat cgtcggcggt cgaagccttg gcgtcgaagc
     2101 gcatgtgcgg ctggttgccg gcggtgcagt agtgcgtggc gcgcagcgcc gatccctcga
     2161 ggaagtacac cgtcaccatc tcgtggggcg tcccggggaa catcttctcc accacagccg
     2221 tcccgccggc cgtgaccgag aaaatcgact tcgccggcgc cttctccgac ccgacgatgg
     2281 tcccttccca ggtgccggcg agcgccttga gccgctcgag tgccgccttg ccgtccagcg
     2341 ccgcgcccgc cgccggggcc gccggtgcgg ctcccggggg agcgccggcg aagacggcca
     2401 tcggggcgag aacgagcaga gcggcgcaac tcaatgcgtg tcgatgcatg tcggttcctc
     2461 cggacagtga ggcggtcggt gccgcccctt cgaaggacct tattacacga acgcccgcgt
     2521 ccctttcaaa cccggggacg ggaaaatggg cgtcaggggc gttcgggcgc cctgtgaagc
     2581 tcgaggacga cgatttccgg ggggaccagg aaccgcaccg ggatgatgct cgtccccagc
     2641 ccggtggcca cgtacaggtg ccgcccctcc tcgatgacgt gccccgcggc gaagcgctgc
     2701 ccgtggaggg aggggacgat cggccgcccg aagaacggca ggcgcacctg cccgccgtgc
     2761 gtgtgtcccg cgagggtgag cgcgacgcgc gccggcactt cgggaaagag gtccgggttg
     2821 tgggtgaaga cgaggatcgg ggcgtcgtcg gtgaccatgg cgagcgcggc gtggagatcg
     2881 tgcggggcgc gcaggaggtc cgagacgccg gtgatccaga acggcgcgtc ggcgccggga
     2941 gggccgctgc cgcctccgag gcggaccgac tcgtcctcga ggacggtgat cccgaaacgg
     3001 cgcagggcgc cgcggatgcg cgggccgttc ctggcgtagt cgtggttgcc gaggaccgcg
     3061 tacacgccga ggggggcgcg caggtcgccg agcagagagg cgaccgtctc cggcgggacc
     3121 tcgtggccgc cgaggacgcc gtgcacgacg tagtcgccga gcaggaggac gaggtgcggc
     3181 tcggcggcat tggtgcggcg gacgatttcg cgcagcttca cggcgtcgtt ccatgccgag
     3241 ccggtgtgca gatccgcgag gagtgctatg cgcaggccgt cgtcttcc
//
LOCUS       DASRIQ010000513         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2279655, whole genome shotgun sequence.
ACCESSION   DASRIQ010000513 DASRIQ010000000
VERSION     DASRIQ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2279655"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VFD06_12535"
     CDS             complement(<1..152)
                     /locus_tag="VFD06_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN04401.1"
                     /translation="MSTAELTDIQGMIRETVRDLARKEFAPRAAKLDETGEFPWENFR
                     KLAELGL"
     gene            247..1395
                     /locus_tag="VFD06_12540"
     CDS             247..1395
                     /locus_tag="VFD06_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN04402.1"
                     /translation="MNGTIPPVAPRKKKALFARRMERLQLSGIRKIFELAARLKDPVN
                     LSLGQPDFDVPIEIQDAAIRAIRSGKNGYTVTEGIPELREKLSEELRRRAGFTEGKVL
                     ITSGVAGAIFLAMGVLVEKGDEVIVPDPYFGMYWNAIRFFAGVPKLLDTYPDFRIKPE
                     KLDRAITPRTKLILFNNPVNPTGIAYRKEEIAAIAEVARRRGVLILSDEIYASYSYDF
                     PHESMRLYSPDCLLVGGFGKAYAITGWRLGFAAGQAEIIDKMTLLQQCSFVSAPSFTQ
                     YAGVAALDVKVSEFRADYRRKRDMVYDELKGRYVIEKPQGAFYFFPKCPGGLKDTEFV
                     RRAIDRGLLIVPGSACSKKSTHFRLSYAVPDPVLERGVDILRKLAKDV"
     gene            1398..2354
                     /locus_tag="VFD06_12545"
     CDS             1398..2354
                     /locus_tag="VFD06_12545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZN04403.1"
                     /translation="MGFRVYRPYPFPDAVDPAIGKELKRLGVAFEPELHAETDAIVCL
                     LATKVDAALLDRVPKLRVIANVAVGYDNIDVPACTARGVVVTNTPDVLTEATADLAWA
                     LLLAAARRVPEGDRFARSGKWKRWEWTMFHGADVHGRTLGILGAGRIGQATGHRAVGF
                     SMQVLYTSRQRRLLFEHTTMARRVDFTTLLRESDFLSIHVPLTRETHHLIGAKELALM
                     KPGAILINTARGPIVDEAALAKALKSGKLAAAGLDVFEKEPKIHPALLKLDNVALLPH
                     IGSATESTRRRMVETALRNCMAALKGEMPPNAVNAHALELKE"
     gene            2356..>2973
                     /locus_tag="VFD06_12550"
     CDS             2356..>2973
                     /locus_tag="VFD06_12550"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZN04404.1"
                     /translation="MLPFYWKLGPLAVTPNELFTVLGAVVGALIIGRRLMALGTNVGG
                     VLDFILTALADGAVGARLYYFLPLWFRGQVTFGTLFSSWADGSGFFGAFIGGTLALAL
                     TARFKKMPVLPTLDEIHGVIPLGFAVGKIGCFLAGCCYGFPSSAGVKFAPGSLCYTTQ
                     LRAGQIPRGAPASLPVHAIPLYDMIYGFAFFGLLWLLRKRSKRPGE"
BASE COUNT          461 a         1007 c         1016 g          489 t
ORIGIN      
        1 aggcccagct ccgcgagctt gcggaagttc tcccagggaa actccccggt ctcgtcgagc
       61 ttcgcggcgc gcggggcgaa ttccttccgg gccaggtccc ggaccgtctc ccggatcatg
      121 ccctggatgt cggtgagttc ggctgtgctc ataccaccat tctatgggac accggataaa
      181 agtcattcct attcctgcgg tgtcaaaccg tgaggactcc agtccattcc ttgccaagga
      241 gacatcgtga acggtaccat ccccccagtg gccccccgga agaagaaggc cctgttcgcc
      301 cgccgcatgg agcgcctcca gctctcgggc atccgcaaga tcttcgagct cgccgccagg
      361 ctcaaggacc cggtgaacct gtccctcggc cagccggact tcgacgtgcc gatcgagatc
      421 caggacgccg ccatccgcgc gatccgctcc gggaagaacg gctacacggt gacggagggg
      481 atccccgagc tgcgcgagaa gctgtccgaa gagctgcgcc gccgcgcggg attcaccgag
      541 gggaaggtcc tgatcacgtc cggcgtcgcg ggcgccatct tcctcgccat gggcgtcctg
      601 gtggagaagg gcgacgaggt gatcgtcccc gacccctact tcggcatgta ctggaacgcg
      661 atccggttct tcgccggcgt tccgaagctg ctcgacacct atcccgactt ccggatcaag
      721 cccgagaagc tggaccgggc gatcacgccg aggaccaagc tgatcctgtt caacaatccg
      781 gtcaacccga ccggcatcgc gtaccggaag gaggagatcg cggcgatcgc cgaggtcgcc
      841 cgccgccgcg gcgtcctgat cctgtcggac gagatctacg cctcctacag ctacgatttc
      901 ccgcacgagt ccatgcggct ctactccccg gactgcctcc tggtcggcgg gttcgggaag
      961 gcgtacgcca tcacgggctg gcggctcggc ttcgcggcgg ggcaggcgga gatcatcgac
     1021 aagatgacgc tcctccagca gtgctcgttc gtctcggcgc cgtcgttcac ccagtatgcg
     1081 ggcgtggcgg cgctcgacgt gaaggtcagc gagttccggg ccgactaccg gcgcaagcgg
     1141 gacatggtct acgacgagct caagggccgg tacgtgatcg agaagccgca gggggcgttc
     1201 tacttcttcc cgaagtgccc cggcggcctg aaggacacgg aattcgtgcg ccgcgcgatc
     1261 gaccgcggcc tcctgatcgt cccgggctcc gcctgctcga agaaatcgac ccacttccgc
     1321 ctgtcgtacg ccgtgcccga cccggtcctc gagcgcggcg tcgacatcct gaggaagctc
     1381 gcaaaggacg tgtgaccatg gggttccgcg tgtaccgtcc gtacccgttc ccggacgcgg
     1441 tcgatcccgc gatcgggaag gagctcaagc ggctcggcgt cgcgttcgag ccggagctcc
     1501 atgcggagac cgacgcgatc gtctgcctgc tcgcgacgaa ggtcgacgcg gcgctgctcg
     1561 accgggtgcc gaagctccgc gtcatcgcga acgtggcggt gggctacgac aacatcgatg
     1621 tccccgcgtg cacggcccgc ggggtggtcg tgaccaacac gcccgacgtg ctcaccgagg
     1681 cgacggccga cctggcgtgg gcgctgctcc tggcggcggc gcgccgggtg cccgaagggg
     1741 accgcttcgc gcggtcgggg aagtggaagc ggtgggaatg gacgatgttc cacggtgccg
     1801 acgtccacgg gcggacgctc ggcatcctgg gcgccggccg gatcgggcag gcgacgggcc
     1861 accgcgcggt cggattctcg atgcaggtgc tctacacgtc gcgccagcgg aggctgctgt
     1921 tcgagcacac gacgatggcg cggcgggtcg atttcacgac gctgctgcgc gaatcggact
     1981 tcctgtcgat ccacgtgccg ctcacccggg agacgcacca cctgatcggc gcgaaggaac
     2041 tggcgctgat gaagccgggg gcgattctga tcaacacggc gcgggggccg atcgtcgacg
     2101 aggcggcgct ggcgaaggcg ctcaagtcgg ggaagctggc cgcggcgggc ctggacgtct
     2161 tcgagaagga gccgaagatc catccggcgc tgctcaagct cgacaacgtg gcgctcctgc
     2221 cgcacatcgg cagcgcgacg gaatcgacgc gccgccggat ggtggagacg gcgctccgga
     2281 actgcatggc ggcgctgaag ggggagatgc cccccaacgc ggtcaacgcc catgcgttgg
     2341 aactgaagga gtaagatgct gccgttctac tggaagctgg gccctctcgc ggtcaccccc
     2401 aacgagctgt tcaccgtgct gggggcggtc gtcggcgcgc tgatcatcgg gcggcggctg
     2461 atggcgctgg ggaccaacgt cggcggcgtg ctcgacttca tcctgacggc cctggcggac
     2521 ggcgcggtcg gcgcccggct gtattacttc ctccccctgt ggttccgggg ccaggtgacg
     2581 ttcggcacgc tcttcagcag ctgggccgac ggctcgggct tcttcggcgc cttcatcggc
     2641 gggacgctcg ccctcgcgct caccgcccgc ttcaagaaga tgcccgtgct gccgaccctc
     2701 gatgagatcc acggagtgat cccgctgggc ttcgcggtgg ggaagatcgg ctgcttcctg
     2761 gcgggctgct gctacggctt tccctcctcg gcgggggtga agttcgcccc cgggtcgctc
     2821 tgctacacca cccagctgcg ggcggggcag atcccgcgcg gcgccccggc gtcgctgccg
     2881 gtccacgcga tcccgctcta cgacatgatc tacggcttcg cgtttttcgg cctgctctgg
     2941 ctcctccgga agcggtcgaa gcggccgggc gaa
//
LOCUS       DASRIQ010000514         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1259591, whole genome shotgun sequence.
ACCESSION   DASRIQ010000514 DASRIQ010000000
VERSION     DASRIQ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259591"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFD06_12555"
     CDS             <1..492
                     /locus_tag="VFD06_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04405.1"
                     /translation="CGLTACEDEGDTVNVNGLDCGLVRAHLDGDWTVAYAPVARTLQN
                     CDDAAFNGTQVSFVAGEVPYPNANVVPSPSSTSFVVEAEGPFGLPDELKVNVEADSCL
                     ALAQIWEDDDSGWVTCLGTADLSSRLLNVICDSFDLDTNLDGSPDTACDLNGSVTATL
                     VLP"
     gene            489..734
                     /locus_tag="VFD06_12560"
     CDS             489..734
                     /locus_tag="VFD06_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HZN04406.1"
                     /translation="MMEPSEIARRIEQGLPGAVADVKGADNHYSARVVSDRFEGKTRI
                     EQHKMIYDLFREEMASQEIHALALKTLTPAQARNEGQ"
     gene            747..1076
                     /gene="grxD"
                     /locus_tag="VFD06_12565"
     CDS             747..1076
                     /gene="grxD"
                     /locus_tag="VFD06_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HZN04407.1"
                     /translation="MAEDPILTDIKKTLGDNTIVLFCKGTKEEPRCGFSMRTIQVFKE
                     MGKPFEVVDVLPDPRIRQVLSAYSSWPTIPQVFIKGQFIGGCDIVTEMFQDGELKKVV
                     DEAFSKV"
     gene            1087..>2054
                     /locus_tag="VFD06_12570"
     CDS             1087..>2054
                     /locus_tag="VFD06_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN04408.1"
                     /translation="MRRPSALRPLPPPRFPAFLLGLCLLAAAVTADAGLSAPKSRDAG
                     LSKDERRVVAAIDARDAAARDLLKAAVDINSGSNNLGGVRAVGALFRKEFDALGFQTS
                     WIDGAAWGRAGHLVARREGRAGAPRVLLIGHLDTVFPRDSPFQRYEPIPGTDEARGPG
                     LLDMKGGDVIMLLALGGLLDARLLDRLSIVAVLTGDEEDSGRPLEAARRDLVEAARRA
                     DIAIGFEDGDGDPRTAVVARRGSGSWLLKVTGTQAHSSQVWSEAVGSGAIYEAARILA
                     GFDAALQGSDLLTVNPGSIVGGTTVTFDDDESRGTAFGKTNVVASSV"
BASE COUNT          330 a          706 c          710 g          308 t
ORIGIN      
        1 tgcggcctga ccgcctgcga agacgagggc gatacggtga acgtcaacgg gctcgactgc
       61 ggcctcgtcc gggcccatct ggacggcgac tggaccgtgg catatgcccc cgtcgcgcgg
      121 acgctgcaga actgcgacga cgccgcgttc aacgggaccc aggtcagctt cgtcgccggc
      181 gaggtgccgt acccgaatgc caacgtggtc cccagcccct cgagcacgtc gttcgtggtg
      241 gaagccgagg gacctttcgg cctgcccgac gagttgaagg tgaacgtcga ggccgactcc
      301 tgcctcgccc tggcgcagat ctgggaggat gatgattccg gctgggtgac gtgcctcggc
      361 accgccgacc tctccagccg gctcctgaac gtgatttgcg attcgttcga cctcgacacc
      421 aacctcgacg gttccccgga caccgcctgc gacctgaacg gcagcgtcac cgcgaccctg
      481 gtgctcccat gatggaaccg agcgagatcg cccggcggat cgagcagggc ctccccggcg
      541 ccgtcgccga cgtgaagggg gccgacaacc actacagcgc gcgcgtggtc tccgaccgct
      601 tcgaggggaa gacgcgtatc gagcagcaca agatgatcta cgacctgttc cgggaggaga
      661 tggcgtcgca ggagatccac gccctggcgc tgaagaccct gacgccggcg caggcccgga
      721 acgaaggaca ataacgagga gatgtcatgg ctgaagatcc gatcctgacc gacatcaaga
      781 agacgctcgg cgacaacacg atcgtcctgt tctgcaaagg caccaaggag gagccgcgct
      841 gcggcttctc gatgcgcacc atccaggtct tcaaggagat ggggaagccg ttcgaggtgg
      901 tggacgttct ccccgacccg cgcatccggc aggtgctgtc ggcctattcg tcctggccga
      961 cgatcccgca ggtgttcatc aaggggcagt tcatcggcgg ctgcgacatc gtcaccgaga
     1021 tgttccagga cggcgagctg aagaaggtcg tcgacgaggc cttctcgaaa gtctgagccg
     1081 tcccgcgtgc gacggccttc tgcgctgcga ccgctcccgc ccccgaggtt tccggcgttc
     1141 ctcctcgggc tctgcctgct cgcggcggcg gtcacggccg acgccggcct gtcggccccg
     1201 aagtcgagag acgccgggct gtcgaaagac gagcgccggg tggtcgccgc gatcgacgcg
     1261 cgtgacgcgg ccgcccgcga tctcctgaaa gccgcggtcg acatcaacag cggctcgaac
     1321 aacctgggag gggtgcgcgc ggtcggcgcg ctcttccgga aggagttcga cgcgctcggg
     1381 ttccagacct catggatcga tggggccgcc tggggccgcg ccgggcacct cgtcgcccgc
     1441 cgcgaggggc gcgccggggc gccgcgcgtc ctgctcatcg gccatctcga caccgtcttc
     1501 cccagggatt cccccttcca gcgctacgag ccgatccccg gcaccgacga agcgcgcggc
     1561 cccgggcttc tcgacatgaa ggggggggac gtcatcatgc tcctggcgct cggcgggctc
     1621 ctcgacgcgc gcctgctcga ccggttgtcg atcgtcgccg tcctcaccgg cgacgaggag
     1681 gattccgggc ggccgctcga ggcggcgcgg cgcgacctcg tcgaggcggc gcgccgggcc
     1741 gacatcgcca tcggcttcga ggacggggac ggcgacccgc gcacggcggt cgtggcgcgg
     1801 cgcggcagcg ggtcatggct cctgaaagtg accggaaccc aggcgcattc gtcgcaggtc
     1861 tggagcgagg cggtcggcag cggcgccatc tacgaggcgg cccgcatcct cgccggtttc
     1921 gatgcggccc tgcagggaag tgatctcctg actgtcaacc cggggagcat cgtcggcggg
     1981 acgacggtca ccttcgacga cgacgagagc cgcggcaccg ccttcggcaa gaccaacgtc
     2041 gtggcttcga gcgt
//
LOCUS       DASRIQ010000515        10715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1767725, whole genome shotgun sequence.
ACCESSION   DASRIQ010000515 DASRIQ010000000
VERSION     DASRIQ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10715
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767725"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFD06_12575"
     CDS             complement(<1..447)
                     /locus_tag="VFD06_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04409.1"
                     /translation="MIRGSLPWMARASWSGGAFGVASAMVLIALLGLKALPALIAAVA
                     IIAIGYLGGVLVAARTRLRDSVRYVLRLRTELRLTQDYLMEQGSSRSLGVWLEAAGAT
                     FKGPLATLQSDARSLAADASLPESTRAAAKSLVDRANAFAEALKPIL"
     gene            complement(444..839)
                     /locus_tag="VFD06_12580"
     CDS             complement(444..839)
                     /locus_tag="VFD06_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZN04410.1"
                     /translation="MGQFEKHVFVCTGGDYCPFDGSVEVHAFLKEGIKARGLKKTVRV
                     NKSGCLDQCGNGPMVVVYPENVWYSHVTLEKAARILEEHLVGGKPVAEYFYVAPPGVN
                     KNPKRITEIEKAREAETAAGAPSGRGLPS"
     gene            complement(915..2147)
                     /locus_tag="VFD06_12585"
                     /pseudo
     CDS             complement(915..2147)
                     /locus_tag="VFD06_12585"
                     /inference="COORDINATES: protein motif:HMM:NF009911.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
     gene            complement(2173..2988)
                     /gene="fdhD"
                     /locus_tag="VFD06_12590"
     CDS             complement(2173..2988)
                     /gene="fdhD"
                     /locus_tag="VFD06_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZN04411.1"
                     /translation="MSTAALETRTLLKVREGQSDASTDDWVVVEEPLEIRVGGETLAV
                     VMRTPGDDLELGAGFALTEGVVGAPEGIGTIRRCPEAKDGGENALDITLAPGFTFDPE
                     RLRRALVASAACGLCGRASLEALAARARASDSTLRVPRSVVLDLPRRLSAAQGVFRRT
                     GALHAAALFDAAGTLVVAREDVGRHNAVDKVIGRAIFDRLPLGDLILLVSGRTSFEIL
                     QKAAVAGIPMVAAVSGPTTLAIETARAFNITLVNFLRPGGMNVASAPERIASS"
     gene            3124..3711
                     /locus_tag="VFD06_12595"
     CDS             3124..3711
                     /locus_tag="VFD06_12595"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZN04412.1"
                     /translation="MPGFILKRVLPLVAVLSLGAIASPVFAQNREKAWEINPYFGYLS
                     FSEVEGQNVIDDTWDLGFRFGYHWTKKQMVEFGFFGGSTDSPDIDVTVDLLGGQVDYI
                     YNFFVHRRDKVVLFAAGGVGLLNTSSFGFVSDPELIGDEVHFSYNLGGGVRFFGGPRA
                     GFRMDLRRVSFSDNDADVTVLEATVGLTVVLGGAY"
     gene            complement(3992..4951)
                     /locus_tag="VFD06_12600"
     CDS             complement(3992..4951)
                     /locus_tag="VFD06_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZN04413.1"
                     /translation="MRRLPASPGSVKGIPLVLAMTLSLSAAACRQDRGVAAAHPPVPE
                     AASTGLDAGGVRDGLNRLVVIPSPPRRIVTLAPNATDTIAALGLGNRLVGVSDFCEPP
                     PEAGEVRRIGGLLTPDLEVIRALKPDLLVGSTSGNDPTLADQAGALGMPLFILNAATV
                     DEVLDGISGLSAALGEEPKGTALVADLRARLDAVARRVAGRPRPRVLYIVWGEPLVVP
                     GSKAFLTDAIRRAGGDSVTDDAPAAHPTYSVEAAIARKPEIILTSEYNRADAERLKTD
                     PAWRSVPAVASGKVYVVGDELVRPGPAVVAGIEEMARRIHPGG"
     gene            5090..6364
                     /locus_tag="VFD06_12605"
     CDS             5090..6364
                     /locus_tag="VFD06_12605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZN04414.1"
                     /translation="MSETIAFLQGNAAWGGTVVGLVVGALLIMLWRGRTAPGRPAPAA
                     RAERGDPAASVLRDLQRTVARLESDNAALSDFMQLLPEFTRRMNSRIERREIPMQMVR
                     IVEQLFAPSQILIFLLEERINKMALVKEVGLPGDAARQVQFDFGEGRLGWVAQNGIAM
                     DLDDFIREKRLTGANFDVPGHFQFSVELCAPMIHEGKVRGVISAGGIKRHYKLEKSIL
                     SLIADLGSIALYNHDLFNKTQEMANCDGLTKLFNKRYFMERLSNVLLEASKGHRPFSL
                     FIFDLDHFKHYNDTQGHQAGDEVLKATGEILRDTVRPDDLACRFGGEEFIVLLAHAPK
                     RGALLAAERIREKIAAHPFAARESQPLKVVSLSGGVATFPDDGLTSADLIAAADAALY
                     RAKKEGRNRVLPSEPKYFSDESDDIVYHPQSG"
     gene            6380..7729
                     /locus_tag="VFD06_12610"
     CDS             6380..7729
                     /locus_tag="VFD06_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN04415.1"
                     /translation="MGAAGTSRKRKFLEELRARGESHDAGALTDLLYDRVTDLPTVPL
                     LLGRIRNMLKESKELGLLSISVLQNEKVEQTLGRQGYESLVRDIASFLVQVKQETLRR
                     EDQLAEVMISGNAFVVLLAPPRGSDQISYPSIDKVRRRVAQSLGRFVRDRVSPSVQER
                     FGIYVGCAVLSNDEALRFERRVYAALEEAFADSIRERNREQRKVSMIFKEVLRTGAVQ
                     AVYQPVVDMVERRAIGFEALTRLTTSSFPGPDQLFKAAYENDSVWKLERLCRERAIRG
                     LKNFRPDHLLFLNMEPDSIYDPALRSEEIFALLGEADLKPSQVVLEITEHSAVQEYEA
                     FRQMLNYFQFHGFRLAVDDVGSGYSGLKSIAELRPNFIKIDMALIRDIHLHPIKQDLT
                     STIARFSSSSGTTLIAEGVETVEELRCLQRIGVRFAQGFLFARPGPPLPTPDLTVLG"
     gene            complement(7850..8047)
                     /locus_tag="VFD06_12615"
     CDS             complement(7850..8047)
                     /locus_tag="VFD06_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04416.1"
                     /translation="MAKAKTRVARKPKKNSYGMIDFSFGNLQYQIDMSRRKVYHRFIE
                     VETAKTSLIMGAYNTVVAQAK"
     gene            8310..9794
                     /locus_tag="VFD06_12620"
     CDS             8310..9794
                     /locus_tag="VFD06_12620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN04417.1"
                     /translation="MSLEGKHILLVDQGADSGATQGVLKQMGARVVAVTDADQAMRET
                     RREEFDLALIGVGLGEKGSEEALRRLSDGARVPSILMLARPHEVEAALRALPKGAADY
                     LLKPVAAAEVEARVGRLLRWQEHDLRARHMQDEMSQRYLVGRVVSRSSGMQRVRQQIV
                     QVAPARSTVLILGESGVGKELVAKAIHYNSPRRAGPFIALNCSAIPETLIESDLFGHE
                     KGAFTGAHERQRGKFELAHGGTLLLDEIGEMGLGTQAKLLRVLEEREFMRVGGSRPVR
                     VDVRVLAASNRDLRDLIARRRFREDLYFRLNVITIRVPPLRGRREDIPELARSLLEQI
                     CRDNGLPAKRLGDGGVRALLRHAWPGNVRELKNVLESAAITRPGEVIQAEDLPDGFRG
                     GAGGSDDDEGEIPSEISLSGMEREAIRRALVRHEGNRTRAARALDIGLRTLQRKIRLY
                     ALGPDGMRGRPRRPQKSTSTPSPGPPVIESVPSRRRTPPRATSS"
     gene            complement(9701..>10715)
                     /locus_tag="VFD06_12625"
     CDS             complement(9701..>10715)
                     /locus_tag="VFD06_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HZN04418.1"
                     /translation="YRIAGRHFTTADNVLLKLVASDGLTGYGCGAPSVEVTGETPQET
                     LRVLRDVLIPLAREPFGDVAALMSRVSVAASAAPAARAAFDMALLDLEGKRAGLPLVH
                     LLGSDRTTLDTSITLGIEDDVARAVAHARRRASEGFRILKVKVGEDWEHDLRLLRALR
                     ETLGPAIVIRADGNEGYDERAAASFLQEAAGLDLELLEQPVPAENLDAMTRLASATTV
                     PIMADEALLTDEDAARLIARKAATLFNVKLMKSGGVLSGLAIARRAETAGIGVMVGCN
                     DESRIAIAAGLHFALAAPAGTRADLDGHLDLQDDVARGGVRLLDGTLSITGGPGLGVD
                     VDF"
BASE COUNT         1613 a         3678 c         3768 g         1656 t
ORIGIN      
        1 caggatcggc ttgagcgcct cggcgaaggc gttggcccgg tcgacgagcg atttcgccgc
       61 ggcgcgcgtg ctctcgggga gcgacgcgtc ggcggcgagg ctgcgcgcgt ccgactgcag
      121 ggtcgcgagc ggccctttga aggtcgcgcc cgccgcctcg agccagacgc ccagcgagcg
      181 cgacgagccc tgctccatca ggtagtcctg ggtgaggcgc agctcggtgc gcagccgcag
      241 cacatagcgg accgagtcac gcagccgcgt gcgcgccgcc accaggacgc cgcccaggta
      301 gccgatggcg atgatcgcca cggcggcgat gagcgccggc agggccttca ggccgaggag
      361 cgcgatgagc accatcgccg aggcgacgcc gaacgcgccg ccgctccacg aggcgcgcgc
      421 catccagggg agggatccac ggatcatgac gggagccccc ttcccgaagg ggcgccggcg
      481 gcggtttccg cctcgcgggc cttctcgatc tccgtgatgc gcttcggatt cttgttgacg
      541 cccggcggcg ccacgtagaa gtactcggcg accggtttcc cgccgacgag gtgctcctcg
      601 aggatgcgcg ccgccttctc cagggtcacg tgcgagtacc agacgttctc ggggtagacg
      661 acgaccatcg gtccgttgcc gcactgatcg agacatcccg acttgttcac gcgcaccgtc
      721 ttcttgagcc cacgggcctt gatcccttcc ttgaggaacg cgtgcacctc gaccgacccg
      781 tcgaacgggc agtagtcgcc gccggtgcac acgaagacgt gtttctcgaa ttgtcccatg
      841 gcggctcgct caggatcgcg aaaaatatca cagccgggcg gtcgcctcaa aacggcggcg
      901 cggcgggcca ccggctacgg cacggcggcc cctccggccg ccggcgatgg tacggcggcc
      961 cctcgaagct ccgcgatcgc cgccacgacc tccccgatct tgttgttcgg ggtggaggcc
     1021 ccggcggtca gcccgatgac cgtccgcccc tccgggagcc acccgtcggt gcgccgctcc
     1081 gtcgtctgcc cgtacggctt gtgccggatc cccccgtcct tctcgatgca ggaggcgtcc
     1141 tcgatgtggt acgtcggcac gctccggccg gcgagcgccg tcaggtggtt ggtgttgctg
     1201 ctgttgtagc cgccgatgac gaccatcagg tccggcttct gcgcgagaag ctcgacgacg
     1261 gcatcctgcc ggtcctgggt ggcgctgcag atggtgtcga aggcgcggaa gtggtcctcg
     1321 atgcccccct ccccgaagcg gtcggcgagc gcccgccgga tctcctcctg gatctgcagc
     1381 gactccgacg acagcatcgt cgtctggttc gcgagcccga cgcgatggag atcccggtcc
     1441 gggtcgaagc cgggggagca ggcgggggag aaccgctcga ggaatgccgc ccggtcgccg
     1501 ccgtggcgga tgtagtcgca gacctgcgcc gattccgtca ggtcgcggac gatgagatag
     1561 cgccctcccg cctggcgcgt cgtgcgtgag gcggtggcgc gcgtttcctc gtgagcatac
     1621 ttgccgtgga tgatggcggt gaagccggag gccgagaagc ggtccacgtt cttccagacg
     1681 ttcagcaccg agccgcacgt cgtgtccacg aggatgcagc cgcgctcctt gagcgcctgc
     1741 atctcctgca cggtcacccc gaacgccggc aggatgacga cgtcctcctt ctgcaccgcg
     1801 tcgaaacccg gcccggagcc gtcggcgtgc cgcaggaagc cgatccccat gtcgctcagc
     1861 cgcctgttga cgtgcgggtt gtggatgatt tcaccggtca gccagatggc cctctcgggg
     1921 aagcgccggc gggtctcgta ggcgtactcg accgcccgct cgacgccata gcagaagccg
     1981 aactcccgcg ccaggaggaa cgtcagctcc cccgcctgaa gccggtgccc cgcggccttg
     2041 agccgctcga cgaggccgct gtggtagtcg gcatgcaggt ggggagagac ctcctccttc
     2101 aggccgaagc cgcgctggaa gtagttctcc gatttggtca tgcggtcgcg cgccccgtcg
     2161 ttgtcttcac cgtcatgagg aggcgatccg ctccggcgcg gaggcgacgt tcattccacc
     2221 cgggcgcagg aagttgacca gcgtgatgtt gaacgcgcgc gccgtctcga tcgcgagggt
     2281 cgtcgggccg gagaccgccg cgaccatcgg gatcccggcc accgccgcct tctggaggat
     2341 ctcgaaggac gtccggcccg agacgagcag gatcaggtcg ccgagcggca ggcgatcgaa
     2401 gatcgcgcgg ccgatgactt tgtccacggc gttgtggcgg ccgacgtcct cacgcgcgac
     2461 gacgagcgtc cccgcggcgt cgaagagagc cgcggcatgc agcgccccgg tgcggcggaa
     2521 cactccctgc gccgcggaga ggcggcgtgg caggtcgagg accaccgacc gcggcacgcg
     2581 gagcgttgag tcgctcgcgc gggcgcgggc ggcgagcgct tcgagcgacg cccggccgca
     2641 cagtccgcag gcggcgctgg cgaccagcgc ccggcgcagg cgctccgggt cgaacgtgaa
     2701 tccgggcgcc agcgtgatgt cgagggcgtt ctcgccgccg tccttcgcct cggggcaccg
     2761 gcggatggtg ccgatccctt ccggggcgcc gacgaccccc tcggtcaacg cgaagccggc
     2821 ccccagctcg aggtcgtcgc cgggcgtgcg catgacgacc gccagggttt cgccgccgac
     2881 gcggatctcg agcggttcct cgaccacgac ccagtcgtcg gtcgaggcgt cgctctgtcc
     2941 ttcgcgcact ttcaggagcg tgcgggtttc caaggcggcg gtggacatgc gcctattgta
     3001 gccaacgacg ccggggacgc ggcgcgaacc ccgcatttcg ccccgccggg gcggcgctcc
     3061 gggcttgaca gggcagggcc cttctgcaaa ggtatgcgcc gatttccgcg ggggtccggg
     3121 acgatgccgg ggttcatcct caagagagtt ctgccgctcg tggccgtcct gtcgctcggg
     3181 gcgatcgcgt cccccgtctt cgcgcagaat cgcgagaagg cttgggagat caatccctac
     3241 ttcggctacc tgtcgttcag cgaagtggag ggccagaatg tcatcgacga cacctgggac
     3301 ctcggcttcc gcttcggcta tcactggacg aaaaagcaga tggtcgagtt cggtttcttc
     3361 ggcggatcca ccgacagccc cgacatcgac gtcaccgtcg atctcctcgg cggccaggtg
     3421 gactacatct acaacttctt cgtgcaccgc cgcgacaagg tggtgctctt cgcggccggc
     3481 ggggttgggc tcctcaacac gagcagcttc ggcttcgtct ccgatcccga gctgatcggc
     3541 gacgaggtgc acttctcgta caacctcggc ggtggcgtcc ggttcttcgg cgggccgcga
     3601 gcaggtttcc gcatggatct gcgccgcgtg agcttcagcg ataacgacgc cgacgtcact
     3661 gtcctcgaag cgacggtcgg cctgaccgtc gtcctcggcg gcgcctacta ggctcccagc
     3721 tcacgagctt cgcaagcgcc ttccggcggt cctttccggc cggttcccaa ttaaagagga
     3781 tggggttcgc gacgacccgc gcgaccggcc gttttcggcc atccgggcgc ggtgctcgtg
     3841 caacaacgac ctcgcgggcg ccgttgtgca aaaaagtctt gatggcacaa ttcaaggcgt
     3901 tccggaggag gggcagacgg gtgccccctc ctcgatcccc cggccgtgcg ggcatcgtgc
     3961 ccgcacggct tgatcgtttt atgggggctt ttcagccgcc cgggtggatg cggcgcgcca
     4021 tctcctcgat gccggcgacg accgctggtc ccggccgtac caactcgtcc cccacgacat
     4081 agaccttccc tgaggcgacc gccgggaccg acctccaggc ggggtcggtc ttcaggcgct
     4141 cggcgtcggc ccggttgtac tcgctcgtca ggatgatctc cggcttgcgg gcgatcgccg
     4201 cctcgacgct gtaggtcggg tgggccgccg gggcgtcgtc ggtcaccgag tccccgccgg
     4261 cccgccggat ggcgtcggtc aggaacgcct tcgagccggg gacgacgagc ggctcccccc
     4321 agacgatgta aaggacgcga ggccgcggcc ggcccgccac ccgccgcgcc acggcgtcga
     4381 gccgggccct gaggtcggcg accagggccg tccccttcgg ttcttcgccg agggcggcgc
     4441 tcagacccga aatcccatcc agcacctcgt ccacggtcgc cgcgttcagg atgaacagcg
     4501 gcatccccaa agcccccgcc tggtccgcca gcgtcgggtc attgccgctc gtcgagccga
     4561 cgaggaggtc cggcttcagc gcccggatca cctccaggtc gggggtcagg agcccgccga
     4621 tccgccggac ctccccggcc tcgggcgggg gctcgcagaa atcgctcacg ccgacgagcc
     4681 ggttcccgag gccgagggcg gcgatggtgt cggtggcgtt cggagcgagg gtcacgatcc
     4741 gccggggcgg gctcggaatc acgacgagcc ggttcagccc gtcccggact ccccccgcgt
     4801 ccaggcccgt cgaagccgcc tccgggacgg gaggatgggc cgccgcgacc ccccggtcct
     4861 gccggcaggc ggcggcggag agcgaaagtg tcatcgccag gacgaggggg atccccttga
     4921 cgctcccagg agacgcgggc aggcggcgca tgcggggact ttacgcggcg tccgcgcgcc
     4981 gccgcaagcg gcgcctgtca agaattgtca ccggggcgga tcggcctgtc gcggcttgaa
     5041 ttgcccggga ccgggccaca cattggcgcg ggaccacagg ccgaaacgcg tgagcgaaac
     5101 gatcgcattt cttcagggga acgctgcctg gggcgggacc gtcgtcggcc tcgtcgtggg
     5161 ggcgcttctc atcatgctgt ggcgcgggcg cacggccccg ggccgccccg ctcccgccgc
     5221 ccgtgccgag cgcggcgatc ccgccgcgtc ggtcctgcgc gatctgcaga ggacggtcgc
     5281 ccggctcgaa agcgacaacg cggcgctctc cgacttcatg cagctcctgc cggaattcac
     5341 ccgccggatg aactcgcgca tcgagcggcg cgagatcccg atgcagatgg tgcgcatcgt
     5401 cgagcagctc ttcgctccgt cccagatcct catcttcctg ctcgaggagc ggatcaacaa
     5461 gatggcgctc gtgaaggagg tcgggctgcc gggcgacgcg gcacgccagg tccagttcga
     5521 cttcggcgag gggcgcctcg ggtgggtggc gcagaacggc atcgccatgg acctcgacga
     5581 cttcatccgg gagaagcggc tgaccggggc gaacttcgac gtgccgggcc acttccagtt
     5641 cagcgtcgag ctgtgcgcgc cgatgatcca cgagggaaag gtgcgcggcg tcatcagcgc
     5701 cggcggcatc aagcgccact acaagctcga gaagtcgatc ctgagcctca tcgccgacct
     5761 cggcagcatc gcgctgtaca accacgacct gttcaacaag acccaggaga tggcgaactg
     5821 tgacgggctg accaagctgt tcaacaagcg gtatttcatg gagcgcctgt cgaacgtcct
     5881 gctcgaggcc agcaaggggc accgcccgtt ctcgctgttc atcttcgacc tcgatcactt
     5941 caagcactac aacgacacgc aggggcacca ggcgggggac gaggtgctca aggcgaccgg
     6001 cgaaatcctg cgcgacacgg tgcgcccgga cgatctggcc tgccgcttcg gcggcgagga
     6061 gttcatcgtg ctcctggcgc acgccccgaa gaggggggcg ctgctggcgg ccgagcggat
     6121 ccgcgagaag atcgccgcgc acccgttcgc cgcgcgcgag tcgcagccgc tcaaggtcgt
     6181 gtcgctcagc ggcggggtcg ccacgttccc ggacgacggg ctgacgagcg ccgatctcat
     6241 cgccgccgcc gacgccgctc tctaccgcgc gaagaaggag gggcgcaacc gcgtcctccc
     6301 gagcgaaccg aaatacttct cggacgagtc ggacgacatc gtctatcacc cgcagagcgg
     6361 ttaggagccc cggaggctga tgggcgcggc cggcacgtca cgcaagcgga agttcctgga
     6421 ggagctccgg gcccgcgggg agtcccacga cgccggggcc ctcaccgatc tgctctacga
     6481 ccgggtcacc gacctgccga ccgtccccct cctgctcggc cgcatccgca acatgctgaa
     6541 ggagtcgaag gagctggggc tcctgtcgat cagcgtcctg cagaacgaga aggtggagca
     6601 gacccttggt cgccaggggt acgagtcgct ggtgcgcgac atcgcctcct tcctggtgca
     6661 ggtgaagcag gagacgctcc ggcgcgaaga ccagctggcc gaggtgatga tcagcggcaa
     6721 cgcgttcgtg gtgctgctgg cgccgccgcg cggcagcgac cagatctcct acccgagcat
     6781 cgacaaggtg cggcgccgcg tcgcccagag tctcggtcgc ttcgtgcgcg accgggtcag
     6841 cccgagcgtg caggagcgtt tcggcatcta cgtcggctgc gccgttctga gcaacgacga
     6901 ggcgctgcgc ttcgagcgcc gcgtctacgc cgccctcgag gaggccttcg ccgactcgat
     6961 ccgcgagagg aacagggagc agcggaaggt ctccatgatc ttcaaggagg tgctgcgcac
     7021 cggcgcggtg caggcggtct accagccggt cgtggacatg gtcgagcggc gcgccatcgg
     7081 gttcgaggcg ctgacccgcc tgacgacgtc gtcgttcccc gggcccgacc agttgttcaa
     7141 ggcggcctac gaaaacgact ccgtctggaa gctggagcgg ctctgccgcg agcgggcgat
     7201 ccgcggcctc aagaacttcc ggcccgacca cctgctcttc ctcaacatgg agccggattc
     7261 gatctacgac ccggcgttgc ggtccgagga gatcttcgcg ctcctggggg aggcggacct
     7321 gaagccgagc caggtcgtcc tggagatcac cgagcactcg gcggtgcagg aatacgaagc
     7381 attccgccag atgctgaact acttccagtt ccacggcttc cggctggcgg tggatgacgt
     7441 cggctccggc tactccggtc tcaagtcgat tgccgagctg cgcccgaact tcatcaagat
     7501 cgacatggcg ctcatccgcg acatccacct gcacccgatc aagcaggacc tgacctcgac
     7561 catcgcccgc ttctcgtcga gcagcgggac gaccctgatc gccgaggggg tggagacggt
     7621 ggaggagctg cgctgcctgc agcggatcgg cgtgcgcttc gcgcagggat tcctgttcgc
     7681 ccggccgggg ccgccgctgc ccacgcccga tctcaccgtc ctgggctgaa aaaaggagag
     7741 ccgcttcatg cggaccgaag cggctcgggt gtggctggtt cctgtctgct gcgaactccg
     7801 cgccggcccg ctcaggccgg tcgcggtgtg aaggctcgtc tggtgctgct tacttcgcct
     7861 gggcgacgac cgtgttgtag gcgcccatga tgagcgacgt cttggccgtc tccacctcga
     7921 tgaagcggtg gtagaccttc cgccggctca tgtcgatctg gtactgcagg ttcccgaagc
     7981 tgaagtcgat catgccgtaa gagttcttct tgggtttccg ggcgacccgc gtcttcgctt
     8041 tggccattga ccaaccctcc ccaggttgaa atgccgtagg agcaacattt ctgccaaccg
     8101 ggtttgcgcg cccggtcggg caacggtccg gcgcgaagcg ggcgtcagtt gggggatttg
     8161 agacgcgtgt cccacgatgc gggtttcgat tgcgttcccc cgagtgcctc gtggaatgcg
     8221 atcggccaaa gtggcgctat ttatccgttg ataataacgc gtcaatatga cagtattgac
     8281 gcggctcgcc gagctggagg gaccgccgga tgtcgctcga aggcaaacac atcctcctgg
     8341 tcgatcaggg tgccgacagc ggggccaccc agggagtcct gaagcagatg ggggcccggg
     8401 tcgtggcggt caccgacgcc gaccaggcga tgcgcgaaac ccgccgcgag gagttcgatc
     8461 tggccctcat cggcgtcgga ctcggggaga aggggagcga ggaggcgctg cggcgcctgt
     8521 cggacggggc gcgtgtcccg tccatcctga tgctcgcgcg gccccacgaa gtggaggccg
     8581 cgctgcgggc gctgccgaaa ggggcggccg actatctcct caagccggtc gcggcggccg
     8641 aggtcgaggc ccgcgtcggg cggctgctcc gctggcagga gcacgacctg cgcgcccggc
     8701 acatgcagga cgagatgtcg cagcgctacc tcgtcgggcg ggtcgtcagc cgttcgtccg
     8761 ggatgcagcg ggtgcggcag cagatcgtgc aggtcgcgcc cgcccgcagc accgtcctca
     8821 tcctcggcga gagcggcgtg ggcaaggagc tggtggcgaa ggcgatccat tacaactcgc
     8881 cgcgccgcgc cgggccgttc atcgcgctca actgctcggc gatcccggag accctcatcg
     8941 agagcgacct gttcgggcac gagaaggggg ccttcaccgg agcgcacgag cggcaacgcg
     9001 ggaagttcga gctggcgcac ggcgggacgc tgctgctcga cgagatcggc gagatgggcc
     9061 tcgggacgca ggcgaagctg ctgcgcgtcc tcgaggagcg ggagttcatg cgggtcggcg
     9121 gctcgcgccc ggtgcgggtg gacgtgcgcg tgctggcggc gagcaatcgc gacctgcggg
     9181 atctcatcgc ccgccgccgc ttccgcgagg atctctactt ccgcctcaat gtcatcacca
     9241 tccgcgtgcc ccctctgcgg ggccggcgcg aggacatccc cgagctggcc cggtcgctgc
     9301 tcgagcagat ctgccgcgac aacgggctgc cggcgaagcg cctgggggat ggcggggtgc
     9361 gcgcgctcct gcggcacgcc tggcccggca acgtccgcga gctgaagaac gtcctcgaga
     9421 gcgccgccat cacccgcccg ggcgaagtca tccaggcgga ggacctgccg gacggcttcc
     9481 gcggcggcgc cggcggctcg gacgacgacg agggagagat cccctcggag atcagcctca
     9541 gcggcatgga gcgggaggcg atccgccggg cgctggtgcg gcacgagggg aaccgcacgc
     9601 gcgccgcgcg cgccctcgat atcgggctgc ggaccctgca gcggaagatc cgcctgtacg
     9661 ccctcggacc ggacgggatg cgcgggcggc cgcgccggcc tcagaagtcg acgtcgacgc
     9721 cgagcccggg gccgccggtg atcgagagcg ttccgtcgag aaggcgcacg ccgccgcggg
     9781 ccacgtcgtc ctgaaggtcg aggtgcccgt cgagatcggc gcgcgtcccg gcgggggcgg
     9841 cgagggcgaa gtgcaggccg gcggcgatgg cgatgcgcga ttcgtcgttg cagccgacca
     9901 tgacgccgat cccggcggtt tccgcccggc gggcgatcgc cagccctgac aggacgcccc
     9961 ccgacttcat cagcttgacg ttgaacagcg tggccgcttt ccgcgcgatc agacgggcgg
    10021 cgtcctcgtc cgtcaggagc gcctcgtccg ccatgatcgg aaccgtcgtg gcggatgcga
    10081 gccgcgtcat ggcgtcgagg ttctcggccg gaaccggctg ctcgagcagc tcgaggtcga
    10141 gaccggcggc ctcctgcagg aaggatgcgg ccgcccgctc gtcgtaaccc tcgttcccgt
    10201 cggcccggat gacgatcgcc ggtccgagcg tctcccgcag cgcccgcagg aggcgcaggt
    10261 cgtgctccca gtcttcgccc accttgacct tgaggatccg gaacccttcg ctggcgcgtc
    10321 gccgggcgtg ggcgacggcg cgtgcgacgt cgtcctcgat cccgagggtg atgctggtgt
    10381 cgagcgtggt ccggtcggag ccgaggaggt gcaccagcgg cagccccgcg cgcttccctt
    10441 cgagatcgag gagcgccatg tcgaaggcgg cgcgcgccgc gggggccgcg gacgcggcca
    10501 ccgacaccct ggacatgagg gccgcgacgt cgccgaacgg ctcgcgcgcc aaagggatca
    10561 ggacgtcccg caggacgcgc agcgtctcct gcggcgtttc gccggtgacc tcgaccgagg
    10621 gagcgccgca gccgtagccg gtcaggccgt cggatgcgac gagcttgagc aggacgttgt
    10681 cagcggtcgt gaagtggcgt ccggcgatcc ggtac
//
LOCUS       DASRIQ010000516         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_448673, whole genome shotgun sequence.
ACCESSION   DASRIQ010000516 DASRIQ010000000
VERSION     DASRIQ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448673"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFD06_12630"
     CDS             <1..335
                     /locus_tag="VFD06_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013604305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZN04419.1"
                     /translation="TDGMLVVPCSGGTLAAIATGVTTNLLHRAAEVTLKERRPLLLAF
                     RETPLSLVHIENMRQATLAGATVVPVMPAFYAGPRSIDDVVTQFTARLLDLLGLGHRL
                     GRRWEGRE"
     gene            364..1260
                     /locus_tag="VFD06_12635"
     CDS             364..1260
                     /locus_tag="VFD06_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HZN04420.1"
                     /translation="MNPGAAERAVPRILGPLGATLSMIKFQHTLFALPFAFTAAILSA
                     GGWPAADKVGWILGAMVGARSAAMVWNRIADLEFDRRNPRTASRPLVTGELGLPFAWA
                     FLVISLALFYGSAAMLNRLALQLATPAALLILSYSYAKRVTWLTHLHLGASLGLAPIG
                     AWVGVTGRIEAAPLILGAAVTAWVAGFDILYACQDIAFDRRAGLQSIPARFGLEAALR
                     VSARLHVATVALFAALPLAAPVGVLWLGGVALVAGLLFYEHRLVRPDDLSRVNAAFFT
                     VNGVVGFVLLAAAAAEVFSRRV"
     gene            complement(1193..2830)
                     /locus_tag="VFD06_12640"
     CDS             complement(1193..2830)
                     /locus_tag="VFD06_12640"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HZN04421.1"
                     /translation="MDTALRGETGGRTPRDLLFSIAEEAIRACDAARAVTRAVGAEPG
                     RIVVGGRPIRTLPQGRLLVLACGKAAEPMYEAFRARLAAAGSKRPVRALIVHPAPAEK
                     RPPAKPEAGRPALRQENVTSVQAEHPVPGKGSFNAGALALRLFASARSGDDVVALISG
                     GGSALLEAPLVPFVTPKEMARLNEVLIVSGAPIGAINTVRKHLSAAKGGRLALLARQA
                     RSLSTLVVCDVDVERFEEVASGPTAPDRTSLDDLVETISRYGLAPALPERLLEGLRTG
                     RLPETPKPKETIFKRAKSILLLSNLDLRNAAVRGGLARGLSAEAMPTEITGPVEEAVE
                     MVARAIEGAPTGLRLLVLGGEPRTVPPAGGQGGRAQDLALRLALRMRNLSVRGWAFIA
                     TGSDGRDGSSPAAGAFVDSSTLERARALGLDPEKMLARGDSYRLFHRLGDDLVTGPTG
                     TNVRDLYLLLTGLPRRERPAPEAASPATPRPVSVVVPGWRPAPPRPAPRPVRPAPPPA
                     PPAPPKSPARREPAKVRRAGKKPRPRPRRGARSRRRR"
     gene            3005..3277
                     /locus_tag="VFD06_12645"
     CDS             3005..3277
                     /locus_tag="VFD06_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZN04422.1"
                     /translation="MNKADLIDKIAKDARISKTASAKALDSLVDGVTKALKKGDRVAL
                     VGFGTFTVSKRKARVGRNPQTGEQIEIKARKVARFRPGKELAGAIR"
     gene            complement(3438..>3923)
                     /locus_tag="VFD06_12650"
     CDS             complement(3438..>3923)
                     /locus_tag="VFD06_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZN04423.1"
                     /translation="EVERRPEGIHVRIDGRDAAIESSGRGALRTVTIDGRAHEAAVWT
                     AAGADASPRGVRAFDVAVGARIVPVRLIDPLRYGNGHVDEAAAEGTIEVRAVMPGKVT
                     AILVAAGDEVGAGAGLLVIEAMKMENEITAPRAGKVASILKAPGEPVEAGALLAVLES
                     L"
BASE COUNT          516 a         1464 c         1398 g          545 t
ORIGIN      
        1 ggaccgacgg catgctagtc gtgccgtgca gcggcgggac gctcgcggcg atcgccaccg
       61 gcgtgaccac caacctcctg caccgggcgg cggaggtgac cctcaaggag cgccggccgc
      121 tcctcctcgc cttccgcgag acgccgctga gcctggtcca catcgagaac atgcgacagg
      181 cgaccctcgc cggcgccacc gtcgtgccgg tgatgccggc cttctacgcc gggccgcgct
      241 ccatcgacga cgtcgtgacg cagttcacgg cgcgtctgct ggatctcctg gggctcgggc
      301 accgtctcgg ccggcgctgg gaggggcgcg agtgatccgt catcggggac gcgcggaaga
      361 gcgatgaacc caggagcagc ggaacgggcg gtgccgcgga tcctcggtcc gctcggcgcg
      421 acgctctcga tgatcaagtt ccagcacacc ctgttcgcgc tgccgttcgc tttcacggcg
      481 gcgatcctgt cggccggcgg ctggccggcc gccgacaagg tcggatggat cctcggagcg
      541 atggtcgggg cgcgcagcgc cgcgatggtc tggaaccgga tcgccgacct cgagttcgac
      601 cgccgcaatc cccgcaccgc ctcgcggccg ctcgtcaccg gcgagctggg cctgccgttc
      661 gcgtgggcgt tcctcgtcat cagcctcgca ctgttctatg gcagcgcggc gatgctcaac
      721 cgtctcgccc tgcagctcgc gactccggcg gcgctgctca tcctgtcgta ctcgtacgcc
      781 aagcgcgtga cctggctgac gcacctgcac ctcggcgcct ccctcgggct cgcgccgatc
      841 ggcgcctggg tcggggtgac cggaaggatc gaggcggcgc cgctgatcct cggggcggcg
      901 gtcaccgcct gggtggcggg gttcgacatc ctctatgcct gccaggacat cgcgttcgat
      961 cgccgcgccg gactgcagtc gatcccggcc cggttcggcc tggaggcggc gctccgcgtt
     1021 tcggcccgcc tgcacgtcgc gaccgtggcg ttgttcgcgg cgctgccgct ggcggcgccg
     1081 gtgggagtcc tctggctggg gggcgtggcc ctcgtcgcgg gacttctctt ctacgagcac
     1141 cggctggtgc ggccggacga cctgagccgc gtcaacgccg ccttcttcac cgtcaacggc
     1201 gtcgtcggct tcgtgctcct cgccgcggcc gcggccgagg ttttttcccg gcgcgtctga
     1261 ccttggcggg ttcgcggcgc gccggagact tcggcggcgc cggcggtgcg ggcggcggcg
     1321 ccggacggac cggacggggg gcggggcgcg gcggcgccgg gcgccatccc ggaacgacga
     1381 ccgagacggg gcgcggcgtg gccggggagg ccgcctccgg ggccgggcgc tcccggcgcg
     1441 gcagcccggt gagcagcagg tagagatcgc gcacgttggt cccggtcgga ccggtcacca
     1501 ggtcgtcccc gagcctgtgg aacagccggt acgaatcgcc gcgcgccagc atcttctccg
     1561 gatcgagacc gagggcccgc gcccgctcga gggtggagga gtcgacgaag gcgccggcgg
     1621 ccggggacga cccgtcgcgt ccgtcggagc cggtggcaat gaacgcccag ccgcgcaccg
     1681 agaggttgcg catgcgcagg gccagccgca gcgccaggtc ctgcgcccgg cccccctggc
     1741 ctcccgcagg aggcaccgtg cggggctcgc cgccgagcac cagcaaccgc aacccggtcg
     1801 gcgccccctc gatggcgcgc gccaccatct cgaccgcctc ctcgaccgga ccggtgatct
     1861 cggtcggcat cgcttcggcc gacagtccgc gcgccagccc tcccctgacc gcggcgttgc
     1921 gcagatcgag gttcgacagg agcaggatcg acttggcccg cttgaagatc gtttccttcg
     1981 gcttcggggt ctccggcagg cgcccggtgc gcagcccctc gagcagccgc tcggggaggg
     2041 ccggcgccag gccgtagcgg ctgatcgtct cgacgaggtc gtcgagcgac gtgcgatccg
     2101 gcgccgtcgg cccggaggcc acctcctcga agcgctcgac gtccacgtca cagacgacga
     2161 gcgtcgacag cgatcgggcc tgtctcgcca gcagcgccag ccggcccccc ttcgccgccg
     2221 acaggtgctt gcgcacggtg ttgatcgcgc cgatcggagc gccggaaacg atcagcactt
     2281 cgttgaggcg cgccatctcc ttgggggtca cgaacgggac gagcggggcc tcgagcagcg
     2341 ccgagccgcc gccggagatc agcgccacca cgtcgtcccc ggagcgggcc gacgcgaaca
     2401 gccgcagcgc cagagcaccg gcgttgaacg accccttgcc cggcaccggg tgctcggcct
     2461 ggacggaggt cacgttctcc tggcgaagcg ccggccggcc ggcctccggt ttcgcgggcg
     2521 gccgcttctc ggcgggggca gggtgcacga tgagcgcgcg caccggccgc ttcgacccgg
     2581 cggcggcgag ccgggcgcgg aacgcctcgt acatcggctc cgccgccttg ccgcaggcga
     2641 gcaccaggag gcgcccctgc gggagcgtcc ggatcggccg cccgccgacg acgatccgcc
     2701 ccggctccgc cccgaccgcc cgcgtcaccg cgcgcgccgc gtcgcaggcg cggatcgcct
     2761 cctcggcaat ggagaagagc agatcgcgcg gcgtgcgtcc tccggtctct cctcgcaaag
     2821 cagtgtccat caatgactcc agaccatggc gctcaaagcg ttcgcggacg ggcgttccgg
     2881 agcgtggcct ctccgtaatc cttgggagac gcgtgttgac acggcttcgg ggggtgggct
     2941 ataaggctga cacgctcgtt gacactggtc aggcagaccc ttcaatcctt gaggaggtgg
     3001 ctcgatgaac aaagcggatc tgatcgacaa gatcgccaag gacgcgcgca tcagcaagac
     3061 ggcgtcggcc aaggccctcg actcgctcgt ggacggcgtc acgaaggccc tcaagaaggg
     3121 ggaccgcgtc gcgctcgtcg gcttcggtac cttcaccgtc tcgaagcgca aggcgcgcgt
     3181 cggacgcaat ccgcagaccg gagagcagat cgagatcaag gcccgcaagg tggcccgctt
     3241 ccgccccggc aaggagctcg cgggggccat ccgctgacgg aaggaccggg cccggacgcg
     3301 cgtcgcggac gccggcggac ccgctgaggg acgggacgag atcgtcccgg accgcgacga
     3361 agtgaatcgg gcggcgcctc cgggcgccgc ctttttttgc gtccgccccg gccgcgccgg
     3421 cctcccaccg tggcacctca cagcgactcc aggacggcca ggagcgcgcc cgcctcgacc
     3481 ggctcccccg gcgccttcag gatcgacgcg accttcccgg cgcgcggggc ggtgatctcg
     3541 ttctccatct tcatcgcctc gatgacgagg agaccggcgc cggcgccgac ctcgtcgccc
     3601 gcggccacga ggatcgccgt caccttcccc ggcatcaccg cgcggacctc gatcgttccc
     3661 tcggccgccg cttcgtcgac atgcccgttg ccgtaccgca gcgggtcgat cagacgcacg
     3721 gggacgatgc gcgcgccgac ggcgacgtcg aacgcccgga ccccgcgcgg gctcgcgtcc
     3781 gcccccgcgg cggtccagac cgccgcttcg tgcgcacggc cgtcgatcgt cacggtgcgt
     3841 aacgccccgc ggccggagga ttcgatcgcg gcatcgcgcc cgtcgatccg gacgtggatc
     3901 ccctcgggcc gtcgctccac ctc
//
LOCUS       DASRIQ010000517        11976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_740399, whole genome shotgun sequence.
ACCESSION   DASRIQ010000517 DASRIQ010000000
VERSION     DASRIQ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 11976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11976
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740399"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFD06_12655"
     CDS             <1..781
                     /locus_tag="VFD06_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZN04424.1"
                     /translation="SPEPVPDYAAAIARGPRGLRLGVPREYFPEGLDGEVAASIRSAI
                     DLARRLGASVEEVSLPHTEYAIPTYYILATAEASSNLARYDGVRYGRRAEGAKDLASL
                     YVRSRSEGFGPEVKRRIMLGTYVLSSGYYDAYYLKGQKVRTLIRRDFDAAFEKVDLLI
                     TPTSPTTAFRLGEKVDDPLSMYLSDIFTVTVNLAGLPGLSLPCGVSRGGLPIGLQIVG
                     RRFAEDAVLRLAAALEAEIGFASRRPRLPERAAAPPADAER"
     gene            778..2865
                     /locus_tag="VFD06_12660"
     CDS             778..2865
                     /locus_tag="VFD06_12660"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZN04425.1"
                     /translation="MSLFPSSVGRALPGIAVLLLLASGPGGGFRAAPEAEAGSNRTKP
                     KPAVRLDKPAGAPSGGAGEVSVPLDEEARAVATSRGDLLLEVKVPRAGVSDAWLGAFV
                     PASAARQSEMAALGGRWPGPGQRLRLRSQSLDETWRVRVVRALFPDAGPGGDTWVHRV
                     GSGKVPVPSQTLARIALWYTGSERNAPALAEVNPQAAQGTRTGQEIAVPRSLLLDGFA
                     RLLPASEEAAAPALALAEMEAGMEPAPAASPGGPAGDAEVPEDDFTEAPAMPETETRP
                     PGAPPGEALPSPAPPPTGAPRRPPVAEGASDLTYGRDAEGPYAAYRLKRGEALYSAVV
                     VRFTGRVDVQEVNDLTVRVAARSGIRDPKHIPIGYKVRIPLDLLLTEYLPRDDPRRQE
                     WERHQAEVERYTNPARSRNLEGVAVILDAGHGGRDRGAAHHGVWEHDYVYDVLCRVKT
                     LLESRTRARVIPTIKDRRDGFKVQEKARLARNQAEVLLTDPPFPLSGTVPSVNLRWYL
                     SNSWYRKLVREGTDPSKIVFTSLHADARHPSLQGAMVYVPGAEYRSGRYGSRREVYAR
                     YREAREEPYVAFTREQRERSEGLSRQFADDLIEAFGDGGVRVHPYLPVRERIIRRGRA
                     WVPAVLRCNIVPVEVLIEISNLSNPQDSKAIADPAYRQKVAEAYVDALGRYFDGTGAT
                     TASRSPAGNPKGR"
     gene            2999..6688
                     /locus_tag="VFD06_12665"
     CDS             2999..6688
                     /locus_tag="VFD06_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN04426.1"
                     /translation="MLLSNAPPTGRTHPGGPKSASPTAHGEGVDLLLGVEGFTYADLH
                     DPRRLADLSDRFDAALLEADAALFDTFDAWRRDPSRFGPVEQSHLLVRVAPHLSRFVS
                     TLFGVGPALETQREKRLSESPVFAFKKEFVQRRVLRRWPEGKKPEAGLPDLEARWRPR
                     RTALFPQIDFERDEERGTAIAALDLMKQHAALPAEASATDRETLEGDLALLEDWCAAR
                     RAAEIDAGRKPSWVSFRMPEDLDYAHLVEVIHPDPGFETMMQGPLPRRRRRDGFKLTD
                     PRFSPREVLDQAHYCVLCHDRDKDSCSKGLKEKQGGIKKNPLGIRLDGCPLDEKISEM
                     HLLTRQGDAIGALALVTIDNPMCPGTGHRICNDCMKGCIFQKQEPVNIPQIETGVLTD
                     VLALPYGFEIWDLLTRWNPLNPKRPHALPYNGRNVLVVGLGPAGYTLAHYLMNEGFAV
                     VGIDGLKIEPPDPDLVGGDGRPPRPIRDAGLLMRELDERPLAGFGGVSEYGITVRWDK
                     NFLTLIQIALTRRRLARFYGGVRFGGTVTLEDAWEMGFDHVAIAAGAGKPTIIDMKNN
                     VMRGVRKASDFLMALQLTGAFKRNALANLEARLPAIVIGGGLTAIDSATELLAYYPVQ
                     VEKILERHETIVHERGETAALLPYTPEERRKLEVALEHGRAIRAERTRAAAAGEEPDF
                     APLVDAWGGVAIAYRKTLQDSPAYRLNHEEVIKAFEEGIRFIEAVDPVEAIPDEHGAL
                     KGVVFKVLKRGEDGSLVDAGERRTLEVKTLLVAAGTSPNVTYEKERPGSFGLDDRRLF
                     FRPHRAERGGDGRFRPVAAGRDDAGAFFTSYESAGRIVSYFGDNHPRYAGNVVKAMAS
                     ARDGYPHIVTLFADALASLDRAHQPERDAALDALYRRLDEALLARVVEVNRLTATIVE
                     AVVRAPQAARRFEPGQFFRVQNYETAAARAGGAPLLIEPLALTGAAVVREDGLLSLIA
                     LEMGTSSRLLSSLQPGDPVVVMGPTGAPTEIPEGSTLLLAGGGLGNAVLFSIAKAAKE
                     RGARVIYFAGYKKAEDLFKREEIEAATDLVVWSVDAGTAIPARRPQDRSFVGNIVQSM
                     TAYGRGLFGAVPISLQDVDRVLAIGSDRMMAAVAKARHGVLAEFLRPDHVGIGSINSP
                     MQCMMKEVCAQCLQRHVDPQTGTETILFTCFNQDQPLDRVDWDHLRQRLAQNTLQEKL
                     GGMWLDHLAAQDGDQGATNLDSMSR"
     gene            complement(6652..7905)
                     /locus_tag="VFD06_12670"
     CDS             complement(6652..7905)
                     /locus_tag="VFD06_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZN04427.1"
                     /translation="MRRNWILPAAALLLLPASARAGNEEVAVLRLPQGEIVWRFLPHD
                     APAHVAYVKSLIARGFYDGTTLHRVIPHFVVQGGDPNSKNDDRSDDGDGEADRRLKAE
                     FSERLHYRPGTVGMARDADPDSGSCQFFIALENLPRLDGRYTIFAEVVEGLEVARRAA
                     AVPRDLNDNPLKRVPVTLRLEKRSVRDAVASRESGSWTSGEVLTGPDKPRPWDSANRL
                     WKTPVVAKAGLRAEGEAAATRLEVAVGADGRVIDVRFPDPAVADPERLQVLPGTWIFE
                     PPLYDGKPQKTRFEIRADGTQPSASTGGGAPIDLAAALAAGAAIAPGTGSIAPPLPAV
                     RVALPAGAKPPARPTRLRLTVGADGMVSDAAVQESCGDAALDAAATEGARALAFAPAT
                     RRRPGKTDAEPLAVYLDIESRFVAP"
     gene            complement(8092..9429)
                     /locus_tag="VFD06_12675"
     CDS             complement(8092..9429)
                     /locus_tag="VFD06_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZN04428.1"
                     /translation="MNPINAMWRRGRSAEATLLALVLLAGCGALAASDLFAAETKPSR
                     TSKPKPPAPPEPSEAPDVPDAPDHDVPLPPRPPKKSGGNKDNPRYVLEEGSTNIGDLY
                     LFKDAVVIRGKQDGDLIVFGRELEVTGTVTGDINAFTQSVDLSGKMGDSVRAMAQSVR
                     LTGDIDGDLVAVGESIVVDKDAHITGDLDAKGARVEMDGTLDGDFTATGGEVHLRGTV
                     GGSAELKGDVIEVDDEARVHGDLNYTSRNRLDLGDESVGGNVAYIPGEAKPKVSSGGF
                     IKWFFWTCTALLAGLVSLAMFRRSAPDIVAAVRTDGLRSAGIGFISAIVIPVALLISC
                     ILIITIPAVILAYVAYFMLLYMAQVPVAVFVGEWLMARLGKTVSPFGAVAVGVPAMYL
                     VFAIPVIGKLALFTVLFTGFGAIVITIWTARQARRAGGMPPPAPMTGATPAPA"
     gene            complement(9573..10358)
                     /locus_tag="VFD06_12680"
     CDS             complement(9573..10358)
                     /locus_tag="VFD06_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZN04429.1"
                     /translation="MPIRVVIVEDEKPARDRLRRLLSEHPDCAIVGEAADAASAAALI
                     DRERPDLCFLDVEMPEGDGFEVLRRVRHAPRVIFTTAYDRYAVPAFEVNSLDYLLKPF
                     SRERFDAALERARAALATGSGAPAPADLLRLIEEIRRAAPSAPAAAGPAAPLRIPVRR
                     ASKIVLLDPGEILWFEAEETIVFARTEEARLLVERPLNDLEELLKPGFFRIHRAYLVN
                     VSRIAEIQPEEGGTARVIMKDAARTPLPLSRRQARLLRDLLPW"
     gene            complement(10377..11513)
                     /locus_tag="VFD06_12685"
     CDS             complement(10377..11513)
                     /locus_tag="VFD06_12685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN04430.1"
                     /translation="MPGPRTTPFGFQVLLWATGNSLAGAAVGLAVNLLGRGTFEPTVL
                     WIGILFGNVVGFTVFLTSTLLPPRLREVGPVLRALLVGLALFSGALAGTALVFYLFPL
                     FVLRDLRQALTVGAINAVLALAVGSVVHVYEAMRWRLAESLREVEEVRLREARLREQA
                     ALAELAALQARINPHFFFNTLNTIASLVEEDPRRAGDIVETLAELFRYTFRAAESRPV
                     RLEEELEFVRRYLTIEQARFGDRLRVVRDVAPAVLRLQVPGLLLQPLVENAVGHGVAP
                     LRRGGTVRIAARLDGEDLVVEVADDGRGLPAGTDPVRPGHGLDNVRQRLQTLYGERGR
                     LTLAAGPGGSGTLAVIRIPVPEAAAAGPGAERETGGRAAGAAVP"
     gene            complement(11524..>11976)
                     /locus_tag="VFD06_12690"
     CDS             complement(11524..>11976)
                     /locus_tag="VFD06_12690"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HZN04431.1"
                     /translation="EVNRLLNGMLYRSTTTARYATLFFALYDADRRLLSYSNAGHFPP
                     LHIARNGEVARLGADGMPLGLFEDATYGQAQRTLAPGDLLVLYTDGIVEAQGTDGVEF
                     GEARFIELLSRHRDADLDDLVLRIMAELERWGGGHSHDDATLVLARVS"
BASE COUNT         1706 a         4365 c         4259 g         1646 t
ORIGIN      
        1 ttcgcccgag ccggtgccgg actacgccgc ggccatcgcc cgcggcccgc gcggcctgcg
       61 cctcggggtg ccgcgcgagt acttcccgga ggggctcgac ggcgaggtgg cggcctcgat
      121 ccgctccgcg atcgacctgg cgcggcggct gggggcgtcg gtggaggagg tctccctgcc
      181 ccataccgag tacgccatcc cgacctacta catcctggcg accgccgagg cgagcagcaa
      241 cctggcccgc tacgacggcg tgcgctacgg ccggcgcgcc gaaggggcga aggacctcgc
      301 ctccctttac gtccgatcgc gcagcgaagg gttcggcccc gaggtgaagc ggcggatcat
      361 gctcgggacc tacgtgctgt cgtccggcta ctacgacgcc tactacctca agggacagaa
      421 ggtccgcacg ctcatccggc gcgacttcga cgcggcgttc gagaaggtcg atctgctgat
      481 cacgccgacg agccccacca ccgcgttccg gctgggtgag aaggtggacg acccgctgtc
      541 gatgtacctc tccgacatct tcaccgtcac cgtcaacctc gccggccttc cggggctgtc
      601 gctgccgtgc ggtgtcagcc gcggcggatt gccgatcggt ctgcagatcg tcggccggcg
      661 tttcgcggag gacgcggtgc tgcggctggc cgcggccctc gaggcggaga tcggcttcgc
      721 gtcgcgccgt ccccgcctgc cggagcgcgc cgccgcgccg ccggcggacg cggagcgttg
      781 agccttttcc cctcctcggt cggccgcgcc ctccctggca tcgcggtgct cctgctcctc
      841 gccagcggac ccgggggagg attccgcgcc gcgccggagg cggaggcggg ctcgaaccga
      901 accaagccga agccggcggt ccggctcgac aagcctgccg gcgccccgtc cggaggcgcg
      961 ggagaggtgt cggtcccgct cgacgaggag gcgcgcgccg tggcgacctc gcgcggcgat
     1021 ctcctcctcg aggtgaaggt cccgcgggcc ggcgtcagcg acgcgtggct cggcgcgttc
     1081 gttccggcct cggcggcgcg gcaatcggag atggccgcgc tcggcggtcg ctggcccggg
     1141 ccgggccagc gcctgcggct gcgctcacag tcgctcgacg agacctggcg ggtgcgcgtc
     1201 gtgcgcgccc tcttccccga cgcgggcccg ggcggcgaca cctgggtgca ccgggtcggg
     1261 agcggcaagg tcccggtccc gtcgcagacg ctggcgcgga tcgccctctg gtacacgggc
     1321 tccgagcgca acgcccccgc tctggcggag gtgaacccgc aggcggcgca agggacgcgg
     1381 acgggccagg agatcgcggt gccgcgatcc cttctgctcg acgggttcgc ccggctcctc
     1441 ccggccagcg aggaggcggc ggcgcccgcg ctggcgctcg cggagatgga ggcggggatg
     1501 gagccggctc cggccgcctc ccccggcggc ccggcggggg acgccgaggt gccggaggac
     1561 gatttcaccg aggccccggc gatgcccgag accgagaccc ggccccccgg ggcgccgccc
     1621 ggagaggcgc tcccctcgcc ggcccctcct ccgaccggcg caccgcgccg gccgccggtg
     1681 gcggaggggg cctcggacct cacctacggc cgcgacgcgg aggggccgta cgccgcctac
     1741 cgcctcaaga ggggcgaagc gctctactcc gcggtggtgg tgcggttcac cgggcgcgtc
     1801 gacgtccagg aggtgaacga cctgaccgta cgcgtcgcgg cgcgaagcgg catccgcgac
     1861 ccgaaacaca tcccgatcgg ctacaaggtg cgtatcccgc tcgacctgct cctgaccgag
     1921 tacctgccgc gggacgaccc gcgccggcag gagtgggagc gccaccaggc ggaggtggag
     1981 cggtacacga accccgcccg ctcgcgcaac ctcgaagggg tggcggtgat cctcgacgcc
     2041 ggccacggcg ggcgcgaccg cggggcggcg caccacgggg tctgggaaca cgactacgtc
     2101 tacgacgtcc tgtgccgcgt caagacgctg ctcgagtcgc gcacgcgggc ccgcgtcatc
     2161 ccgacgatca aagaccgacg cgacgggttc aaggtgcagg agaaggcgcg tctggcgcgc
     2221 aaccaggcag aggtgctgct caccgatccg ccgttcccgc tctcggggac ggtgccgagc
     2281 gtcaacctgc gctggtacct gtcgaattcg tggtaccgca agctcgtgcg cgaggggacc
     2341 gatccatcca agatcgtgtt cacctccctg cacgccgacg cgcggcaccc gtcgctgcag
     2401 ggggcgatgg tgtacgtccc gggtgcggag taccggagcg ggcgctacgg cagccggcgc
     2461 gaggtgtacg cgcgctaccg cgaggcgcgc gaagagccgt atgtcgcctt cacgcgggag
     2521 cagcgggagc gttccgaggg cctgtcgcgg cagttcgccg acgacctcat cgaggcattc
     2581 ggcgacggcg gcgtgcgggt ccacccgtac ctgccggtgc gggagcggat catccggcgc
     2641 ggccgggcct gggtcccggc ggtgctgcgc tgcaacatcg tgccggtgga ggtcctgatc
     2701 gagatcagca acctcagcaa cccgcaggac agcaaggcga tcgccgatcc ggcctaccgc
     2761 cagaaggtcg ccgaggccta tgtcgacgcc ctggggcggt atttcgacgg gacgggggcc
     2821 accaccgcct cccgctcccc tgccggaaac cccaagggac gttgaattcc ccgcggtctc
     2881 gatggacatt tgacacgacc cggcggctcg gccaatatga cctctttgtc ccgccgcatg
     2941 ccgcgtcccc cttcgggttc gggccggact gcggccgcga ggtgaaacac aagtgtcgat
     3001 gctcctgagc aacgctccgc cgacggggcg cacccatccc ggcgggccga agtccgcgtc
     3061 cccgaccgcg cacggggagg gggtcgacct gctgctcggc gtggaggggt tcacctacgc
     3121 cgacctgcac gacccgcggc ggctcgccga tctctcggac cgcttcgacg cggccctcct
     3181 cgaagcggac gcggcgctct tcgacacctt cgacgcctgg cggcgcgacc cgtcccggtt
     3241 cggcccggtg gagcagtcgc acctcctcgt gcgggtcgcg ccccatctga gccggttcgt
     3301 ctcgaccctc ttcggcgtcg gccccgcact cgagacgcag cgggagaaac ggctttccga
     3361 atcccccgtg tttgccttca agaaggagtt cgtgcagcgc cgcgtgctgc gccgctggcc
     3421 ggaaggaaag aagccagagg ccgggctccc tgacctcgag gcgcgctggc gcccacggcg
     3481 caccgccctc ttcccgcaga tcgacttcga gcgggacgag gagcgcggga ccgcgatcgc
     3541 cgccctcgat ctgatgaagc agcacgccgc cctgccggcc gaagcctccg cgaccgatcg
     3601 cgagacgctc gaaggggatc tggcgctcct cgaggactgg tgcgccgccc ggcgcgccgc
     3661 cgagatcgac gccggccgga agccgtcctg ggtgtccttc cggatgccgg aggacctcga
     3721 ctacgcccac ctcgtcgagg tgattcaccc ggaccccggc ttcgagacga tgatgcaggg
     3781 accgctgccg cgccgccggc gccgcgacgg cttcaagctg accgaccccc gcttctctcc
     3841 ccgcgaggtc ctcgaccagg cgcactactg cgtcctctgc cacgaccgcg acaaggactc
     3901 ctgctcgaag gggctgaagg agaagcaggg ggggatcaag aagaacccgc tcggcatccg
     3961 gctcgacggc tgcccgctcg acgagaagat ctccgagatg cacctcctga cgcgccaggg
     4021 ggacgcgatc ggggcgctcg ccctggtcac catcgacaac ccgatgtgcc ccggcaccgg
     4081 gcaccgcatc tgcaacgact gcatgaaggg ctgcatcttc cagaagcagg agccggtgaa
     4141 catcccgcag atcgagaccg gcgtcctcac cgacgtgctg gcgctgccgt acggcttcga
     4201 gatctgggac cttctgaccc gctggaaccc gctcaacccg aagcggccgc acgcgctgcc
     4261 gtacaacggc aggaacgttc tcgtcgtcgg gctgggaccg gccggctaca cgctggcgca
     4321 ctacctcatg aacgaggggt tcgccgtcgt cggtattgac gggctcaaga tcgagccgcc
     4381 cgatcccgat ctcgtcggcg gcgacggccg gccgccgcgg ccgatccggg acgccgggct
     4441 cctgatgcgc gagctcgatg agcgtccgct cgccggcttc ggcggcgtct ccgaatacgg
     4501 catcaccgtg cggtgggaca agaacttcct gaccctcatc cagatcgccc tgacgcggcg
     4561 gcgcctggcc cgcttctacg gcggcgtccg cttcggcggc accgtgacgc tggaggacgc
     4621 ctgggagatg gggttcgacc acgtcgccat cgcggccggg gccggcaagc cgacgatcat
     4681 cgacatgaaa aacaacgtca tgcgcggcgt gcgcaaggcg tcggacttcc tgatggcgct
     4741 gcagctcacc ggcgccttca agcggaacgc gctcgccaac ctcgaggcgc gcctccccgc
     4801 gatcgtcatc ggcggcggtc tgacggcgat cgactcggcg accgagctgc tcgcctacta
     4861 cccggtccag gtggagaaga tcctcgagcg ccacgagacg atcgtgcacg agcgcggcga
     4921 gacggcggcg cttctgccgt acacccccga ggagcgccgg aagctcgagg tcgccctcga
     4981 gcacggccgg gcgatccgtg ccgaacggac gcgggccgcc gccgcggggg aggagcccga
     5041 tttcgctccg ctcgtggacg cgtggggcgg cgtggcgatc gcctaccgca agacgctcca
     5101 ggattctccc gcctaccgcc tcaaccacga ggaggtcatc aaggcgttcg aggaggggat
     5161 ccgcttcatc gaggcggtcg atccggtcga ggcgatcccg gacgagcacg gcgccctgaa
     5221 gggcgtggtc ttcaaggtcc tgaagcgcgg cgaggacggt tcgctcgtcg acgccggcga
     5281 gcggcggacg ctcgaggtga agacgctgct cgtcgccgcc gggacgagcc cgaacgtcac
     5341 ctacgaaaag gagcggcccg gatcgtttgg tctggacgat cgccgcctgt tcttccgccc
     5401 gcaccgggcg gagcgcggcg gggacgggcg cttccgcccg gtcgccgcgg ggcgcgacga
     5461 cgccggggcc ttcttcacgt cctacgaatc ggccgggcgc atcgtgtcgt acttcggcga
     5521 caaccacccg cgctacgccg gcaacgtcgt caaggcgatg gcgtcggccc gggacggcta
     5581 cccgcacatc gtcacgctgt tcgccgacgc gctcgcctcg ctcgatcggg cccaccagcc
     5641 ggagcgggac gccgccctcg acgcgctcta ccggcggctc gacgaggcgc tgctcgcccg
     5701 cgtcgtcgag gtcaaccgac tgaccgcgac catcgtcgag gcggtggtgc gcgccccgca
     5761 ggcggcccgc cgcttcgagc cgggtcagtt cttccgggtg cagaactacg agaccgccgc
     5821 ggcgcgcgcg ggcggcgcgc cgctcctcat cgagccgctg gccctcaccg gcgccgcggt
     5881 cgtgcgcgag gatggcctcc tgagcctcat cgccctcgag atggggacct cgtcgcggct
     5941 gctctcgtcg cttcagcccg gcgacccggt cgtggtcatg ggtccgaccg gcgctccgac
     6001 cgagatcccg gaggggtcga cgctcctcct cgccgggggg gggctcggca acgcggtcct
     6061 tttctcgatc gccaaggcgg ccaaggagcg cggcgcgcgc gtcatctact tcgccggata
     6121 caagaaggcg gaggacctgt tcaaacgcga ggagatcgag gcggcgaccg acctcgtcgt
     6181 ctggagcgtg gacgcgggga ccgccatccc cgcccgccgg cctcaggacc gatcgttcgt
     6241 cgggaacatc gtgcagtcga tgaccgccta cggacgcggc ctcttcggag cggtgccgat
     6301 ctcactgcag gacgtcgacc gggtcctcgc gatcggctcc gaccggatga tggcggcggt
     6361 ggcgaaggcg cgccacggcg tcctcgccga gttcctgcgc cccgaccacg tcggcatcgg
     6421 atcgatcaac tcgccgatgc agtgcatgat gaaggaagtc tgcgcccagt gcctgcagcg
     6481 ccacgtcgat ccccagaccg gcacggagac gatcctcttc acctgcttca accaggacca
     6541 gccgctcgac cgggtggact gggatcacct gcgccagcgc ctggcgcaga acacgttgca
     6601 ggagaagctc ggcgggatgt ggctcgacca cctggccgcg caggacggcg atcagggggc
     6661 gacgaacctc gactcgatgt cgaggtagac cgccaagggc tcggcatcgg tcttccccgg
     6721 ccggcggcgc gtcgccggtg cgaacgccag ggcgcgcgcc ccctccgtcg ccgcggcgtc
     6781 gagggccgcg tcgccgcacg actcctgcac cgccgcgtcg ctcaccatcc cgtccgctcc
     6841 caccgtcagc cgcagccgcg tcggacgggc cggcggcttc gccccggccg ggagcgccac
     6901 gcgcaccgcc gggagcggcg gtgcgatcga tcccgtcccg ggcgcgatcg ccgctccggc
     6961 tgccagggcc gccgcgagat cgatcggcgc cccgccaccg gtggacgcgg acggctgcgt
     7021 tccgtcggcg cggatctcga accgggtctt ctgcggcttc ccgtcgtaga gcggcggctc
     7081 gaagatccag gtgccgggta agacttgtaa gcgctccgga tccgccaccg ccggatcggg
     7141 gaagcggacg tcgatcaccc ggccgtcggc tccgaccgcc acctcgagcc gcgtcgcggc
     7201 cgcctccccc tcggcgcgca gccccgcctt cgcgacgacc ggcgtcttcc acaggcggtt
     7261 ggcggaatcc cacgggcgcg gcttgtcggg gccggtgagc acctcgccgg aggtccacga
     7321 cccggattcg cgcgacgcga ccgcgtcgcg cacgctgcgc ttctccagcc ggagggtgac
     7381 cgggacgcgt ttcagggggt tgtcgttgag atcgcgcggc accgcggcgg cgcggcgggc
     7441 cacctccagc ccctccacga cctccgcgaa gatggtgtag cggccgtcga gacggggcag
     7501 gttctcgagc gcgatgaaga actggcagga gccgctgtcg gggtcggcgt cgcgcgccat
     7561 gccgaccgtt cccggccggt agtggaggcg ctcgctgaat tccgccttga gtcggcgatc
     7621 ggcctcgccg tcgccgtcgt cggagcggtc gtcgttcttc gagttgggat cccccccctg
     7681 gacgacgaaa tgggggatca cccggtgcag cgtggtgccg tcgtagaagc cccgggcgat
     7741 caggctcttg acgtaggcga cgtgcgccgg cgcgtcatgc ggcaggaagc gccagacgat
     7801 ttccccctgc ggcagccgca ggaccgcgac ctcttcgttg ccggcccgtg cgctcgccgg
     7861 caggaggagg agcgcggcgg ccggcaggat ccagttccgg cgcatcgccg ggactctcgc
     7921 ctttccggat gcggccgtca agtccggccg ccgcgtctcc ttcccgccag gggcgtcaga
     7981 acaccacgag gcagaggaac acgattccca gcaacagcac ggtgcgcgat tccatgtgcc
     8041 ttcctccccg tttcgttgaa ccgtctcgat gctctcgttc cgtccgtccc gtcacgccgg
     8101 ggcgggggtg gcgccggtca tcggcgccgg cggaggcatg ccgccagccc gacgcgcctg
     8161 ccgcgccgtc cagatggtga tcacgatcgc cccgaacccg gtgaacagca cggtgaacag
     8221 cgcgagcttg ccgatcaccg ggatggcgaa caccaggtac atcgccggca cgccgaccgc
     8281 cacggcgccg aacgggctca cggtcttgcc gaggcgggcc atgagccatt cgccgacgaa
     8341 caccgccacc ggcacctgcg ccatgtacag cagcatgaag taggcgacat aggcgaggat
     8401 caccgccggg atggtgatga tcaggatgca ggagatcagc agggccaccg ggatgacgat
     8461 ggcgctgatg aagccgatcc ccgcgctcct caaaccgtcg gtgcgcaccg ccgccacgat
     8521 gtcgggggcg ctgcggcgga acatggcgag cgacacgagc cccgcgagga gcgccgtgca
     8581 ggtccagaaa aaccacttga tgaacccgcc gctggagacc ttcggcttcg cctcgccggg
     8641 gatgtaggcc acgttcccgc ccacgctctc gtcaccgaga tcgaggcggt tgcgcgaggt
     8701 gtagttcaga tcgccgtgca cacgcgcctc gtcatcgacc tcgatcacgt cccccttcag
     8761 ctcggcgctc ccgcccactg tgccgcgcag gtgcacctcg ccgccggtgg cggtgaagtc
     8821 accgtccagt gtgccgtcca tctccacgcg ggctcccttg gcgtcgagat cgccggtgat
     8881 gtgggcgtcc ttgtccacga cgatcgactc cccgaccgcc accaggtcgc cgtcgatgtc
     8941 accggtgagc cggacgctct gcgccatggc gcgcaccgag tcccccatct tcccgctcag
     9001 gtcgaccgac tgggtgaagg cgttgatgtc gccggtcacc gtcccggtca cctcgagctc
     9061 gcgcccgaac acgatgaggt cgccgtcctg cttgccgcgg atcaccaccg cgtccttgaa
     9121 caggtacagg tcgccgatgt tggtgctccc ctcttcgagg acgtagcggg ggttgtcctt
     9181 gttcccgccc gacttcttcg gcggacgcgg ggggagcggc acgtcgtggt cgggggcgtc
     9241 gggaacgtcc ggcgcctcgg acggctccgg cggagccggc ggcttcggct tcgacgtccg
     9301 ggacggcttc gtctccgccg cgaagagatc ggaggcggcg agtgcgccgc atccggcgag
     9361 cagcactagc gccaggaggg ttgcttcggc gcttcgtcca cgtctccaca tcgcgttgat
     9421 ggggttcatg ggacctctcc tctcgcggga tggaatacgg cattccgggc ggccgatcca
     9481 cccgtttcgg gcggacggcc ggaaccggct ctgaacggtc gttccggggg gttgaacggt
     9541 gctcactttt cggggcgaat ggccgtccag gctcaccagg ggagcaggtc ccggagcagc
     9601 ctggcctggc ggcgcgacag cggcaggggg gtgcgggcgg cgtccttcat gatcacccgg
     9661 gccgtccctc cctcctccgg ctggatctcg gcgatgcgcg acacgttgac gagataggca
     9721 cggtggatgc ggaagaaccc cggcttcagg agctcctcca ggtcgttgag cgggcgctcc
     9781 accagcagcc gggcctcttc ggtgcgcgcg aagacgatcg tctcctccgc ctcgaaccag
     9841 aggatctcgc cggggtcgag gagcacgatc ttgctggcgc gccgcaccgg gatccggagc
     9901 ggcgcggccg ggcccgcggc ggcgggggcg gagggggcgg ccctgcggat ctcctcgatg
     9961 aggcggagca ggtcggccgg cgccggggcg ccgctccccg tcgcgagcgc cgcgcgcgcc
    10021 cgttcgagcg ccgcgtcgaa gcgctcccgg ctgaacggct tcaggaggta gtcgagcgag
    10081 ttcacctcga acgccggcac ggcgtagcga tcgtacgcgg tcgtgaagat cacccgcggc
    10141 gcgtgccgca cccggcgcag cacctcgaag ccgtccccct ccggcatctc gacgtcgagg
    10201 aagcacaggt ccggccgctc ccgatcgatg agcgccgcgg cgctcgccgc gtcggccgcc
    10261 tccccgacga tggcgcagtc cgggtgctcc gacagcagcc gccggagccg gtcgcgcgcc
    10321 ggcttctcgt cctcgacgat caccacccgt atcggcatcg tgtgagcgcg ctccgcctag
    10381 gggaccgccg ccccggccgc ccgccctccc gtttcgcgct ccgccccggg tcccgccgcg
    10441 gccgcctccg gcaccgggat gcggatcacc gccagcgtcc cgctcccccc cgggcccgcg
    10501 gcgagcgtca ggcggccgcg ctcgccatag agcgtctgca ggcgctggcg cacgttgtcg
    10561 aggccgtgcc cggggcgcac cgggtcggtc ccggccggaa gcccgcgccc gtcgtcggcg
    10621 acctcgacca cgaggtcctc tccgtcgagg cgcgcggcga tgcgcaccgt cccgccgcgc
    10681 cgcagcggcg ccacgccgtg gccgaccgcg ttctccacca gcggctgcag cagaagcccc
    10741 ggcacctgga ggcgcagcac cgccggggcc acgtcgcgca ccacccgcag ccgatcgccg
    10801 aagcgggcct gctcgatcgt caggtagcgg cgcacgaact ccagctcctc ctccagccgg
    10861 accgggcgcg actcggcggc gcggaaggtg tagcggaaca gctcggcgag cgtttcgacg
    10921 atgtcgccgg cgcggcgcgg gtcctcctcc acgagcgagg cgatggtgtt gagggtgttg
    10981 aagaagaagt gcggattgat gcgggcctgg agcgccgcca gctcggcgag cgccgcctgc
    11041 tcccgcaggc gggcctcgcg cagccgcacc tcctccacct cgcgcagcga ctcggccagc
    11101 cgccaccgca tcgcctcgta gacatgcacg acgctgccga ccgcgagcgc gagcacggcg
    11161 ttgatcgccc cgacggtcag cgcctgccgc aggtcgcgca gcacgaagag cgggaagagg
    11221 tagaagacga gcgccgtgcc ggcgagcgcc ccggagaaga gcgccagccc gaccaggagc
    11281 gcccgcagca ccgggccgac ctcgcgcagg cgcggcggca ggagggtcga ggtcaggaag
    11341 accgtgaacc cgacgacgtt gccgaacagg atcccgatcc agaggacggt cggctcgaag
    11401 gtgccgcggc cgaggaggtt caccgccagg ccgaccgcgg cgccggcgag ggagttgccg
    11461 gtcgcccaga gcagcacctg gaacccgaac ggggtggtgc gcggaccggg catcagagga
    11521 tcctcaactg acgcgcgcca ggacgagggt ggcgtcgtcg tgggagtgac cgccgcccca
    11581 gcgctccagc tccgccatga tccggaggac gagatcgtcc agatcggcgt cgcggtgccg
    11641 gctcaggagc tcgatgaagc gcgcctcgcc gaactcgacg ccgtcggtcc cctgcgcctc
    11701 gacgatcccg tcggtgtaca ggacgagcag gtccccgggc gcgagcgtgc gctgcgcctg
    11761 gccgtaggtc gcgtcctcga acaggccgag cggcatgccg tcggcgccga gccgcgccac
    11821 ctcgccgttg cgggcgatgt ggagcggcgg gaagtgcccg gcgttgctgt aggacagcag
    11881 ccgccggtcg gcgtcgtaga gggcgaagaa cagggtcgcg tagcgcgccg tggtggtcga
    11941 gcggtacagc atgccgttga gcaggcggtt gacctc
//
LOCUS       DASRIQ010000518         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3076435, whole genome shotgun sequence.
ACCESSION   DASRIQ010000518 DASRIQ010000000
VERSION     DASRIQ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3076435"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..2530)
                     /locus_tag="VFD06_12695"
     CDS             complement(140..2530)
                     /locus_tag="VFD06_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04432.1"
                     /translation="MKTLAMDVRFGLRMLLRSPAITLVAILTLAIGIGGNTVLFSVVD
                     GVLLRPLPYRDPDRLVVLRDVQPAVPSAPTSYPEYLAWSQESGLFESLAPAFTTTVSL
                     TGSGEPESLIALRASASLLPMLGVTPILGRPHTHAEDARNGERVVLVGEGLWQRRFGA
                     DPGLVGRAITLEGEPWTVIGVMPRSFRFLRPADLFMPLRLSVENAPQGLHFLPVVGRL
                     RPGLPLEQAQRDAEAIAPRIAKELDSGHGVKLPGLKEWIVGDTSRTLALLGAALGFVL
                     LIACANVAGLLLARAAGRRQEVAIRRAIGAGRGDLVRQFLTESLLLALVGGVLGSLLA
                     VWGIDAFKALPEAGIPRLQDVRVDATVLLFTLGLSLMTGLLFGLAPALESISGSMPQH
                     LKGAAGLAGDTRRVQRMRAALVVGQVSLSLLLLVGAGLLARSLVGVLRSDRGFDPSNV
                     MSMELVLPARQVSEPADQARFFSDLLDRLRGLPGVEVAATCGHLPLSGSNTNGDILIE
                     GRPAAEGETRLSELRRVSEDYFTVLRIPVLRGRAFTERDALGAPAVAIVNQAFVDRFL
                     PGQEAIGRRVDAQWETNGWQEIVGVVGDIKHDALDAPPLPELYVPFRQVPMAGMVAVV
                     RSPVDPTALSSALRAQVYAVRPDQPVGEMRSMDDVLHASVAPRRLTASMVGGFAWMAL
                     FLASIGIYGLLAFWVSERRREIGLRMALGARPRQVLRLVVARGLTLTGAGLVIGLVAS
                     AGLTRLLGGFLYGVGPADPPTLVATPLLLGGVALLASWLPARRAMRIDPATALRSD"
     gene            complement(2647..3117)
                     /locus_tag="VFD06_12700"
     CDS             complement(2647..3117)
                     /locus_tag="VFD06_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HZN04433.1"
                     /translation="MSMALVFAVAAGVAVAAAALAILSARSRAGAPAPGAADVGTLSA
                     CPGSPNCVTSLAADPRHAVDPIRFEGPPAIAWERLRTLLASLPQARTAAERPGYLHVE
                     FRSRFFGFVDDAEFLLDASNQRIDVRSASRVGWSDLGANRRRMTLIARRFDDSA"
     gene            complement(3131..>3506)
                     /locus_tag="VFD06_12705"
     CDS             complement(3131..>3506)
                     /locus_tag="VFD06_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN04434.1"
                     /translation="IDLLTADRVVGYNIDRFDLGVLRGYTDRDLSRVRTLDMLSVIYS
                     KLGFRLKLSDVGEATLGVGKSGDGLQSLQWWKEGRIDLIEEYCRKDVEVTRSVYRFGA
                     TNGYILYRDRDGRALRLPVDWK"
BASE COUNT          571 a         1237 c         1273 g          425 t
ORIGIN      
        1 cgatgatcgg ctcgtgatga tgtgggacga tgcgatggga cctgtgcatt tctagcacgc
       61 atcccggcgc ccgatcaagg cggaagtcca gcggatgctc tctgcgcgcg ggaggtgctc
      121 cctccggacg gggcgggagt cagtcgctgc ggagcgcggt ggccgggtcg atgcgcatgg
      181 cgcgccgggc cggcagccag gaggcgagga gcgccacacc cccgaggagg agcggcgtcg
      241 ccacgagggt cggagggtcg gccggcccca cgccgtagag gaagccgccg agcagccgcg
      301 tcagcccggc cgaggcgacg agcccgatca cgagcccggc gccggtcagg gtcagcccgc
      361 gggccacgac gagtcggagg acctggcgtg gtcgcgctcc gagcgccatg cgcaggccga
      421 tctcgcgccg gcgctccgac acccagaacg ccagcaggcc gtagatgccg atcgaggcca
      481 ggaagagggc catccaggcg aaccctccga ccatcgacgc ggtcagccgg cgtggcgcga
      541 ccgaggcatg cagcacgtcg tccatgctgc gcatctcccc gaccggctga tcggggcgca
      601 ccgcgtaaac ctgggcgcgc agggccgacg aaagcgccgt cgggtcgacg ggcgagcgca
      661 ccaccgcgac catccccgcc atcggcacct ggcggaacgg cacgtacagc tccggaagcg
      721 gcggcgcatc gagcgcatcg tgcttgatgt cgccgacgac gccgacgatc tcctgccagc
      781 cgttcgtttc ccactgcgcg tcgacgcgcc ggccgatcgc ctcctgcccc gggagaaagc
      841 gatcgacgaa cgcctggttg acgatcgcca ccgccggcgc tcccagcgcg tcgcgctccg
      901 tgaaggcgcg tccgcgcagc acggggatgc gcagcacggt gaagtagtcc tcgctgaccc
      961 gccgcagctc ggagagccgc gtttcgcctt cggccgcggg gcgcccttcg atgaggatgt
     1021 cgccgttggt gttgctccca ctgaggggca ggtggccgca ggtcgccgcc acctcgaccc
     1081 cgggcaggcc gcgcagccga tcgaggagat cggagaagaa acgcgcctga tcggcgggct
     1141 cgctcacctg ccgcgccggg agcaccagct ccatgctcat cacgttcgaa gggtcgaacc
     1201 cgcggtcgga tcgcagcacc ccgacgaggc tgcgggccag aagtcccgcg ccgacgagca
     1261 gcaggagcga cagggagacc tgccccacga ccagggcggc gcgcatccgc tggacgcggc
     1321 gcgtgtcccc cgcgagtccc gccgccccct tgaggtgctg cggcatcgag ccggagatgc
     1381 tctcgagcgc cggcgcgagc ccgaagagga gcccggtcat gagcgacaga ccgagcgtga
     1441 acagcagcac cgtggcgtcc accctgacat cctggagccg cgggatgccc gcctcgggca
     1501 gcgccttgaa cgcatcgatc ccccagacgg ccagaagcga tccgaggacc ccgccgacga
     1561 gcgccagcag cagactctcg gtgaggaact gccggacgag gtcacctcgc ccggcgccga
     1621 tcgcccggcg gatcgccacc tcctgccggc ggcccgcggc gcgcgccagg agcagccccg
     1681 cgacattggc gcaggcgatc aggagcacga atcccagcgc cgcgccgagc aacgcgaggg
     1741 tgcgcgaggt gtctccgacg atccattcct tcaacccggg gagcttcacg ccgtgccccg
     1801 agtcgagctc cttggcgatg cggggcgcga tggcctcggc gtcacgctgg gcctgctcga
     1861 gaggcaggcc ggggcgcagc cgtcccacga ccggaaggaa atgcagcccc tgcggcgcat
     1921 tctcgacgct caagcgaagc ggcatgaaga ggtccgccgg gcgcaggaag cggaaggacc
     1981 gcggcatcac gccgatcacc gtccacggct ctccctcgag cgtgatggcg cgcccgacga
     2041 gccccggatc ggcgccgaaa cgccgctgcc acagcccttc gccgacgagc acgacccgct
     2101 ccccgttgcg ggcgtcctcc gcgtgggtgt gcgggcggcc gaggatcggc gtcacgccga
     2161 gcatcggcag caggctggcg ctggcgcgca gggcgatcag gctttcgggc tcccccgagc
     2221 cggtgagcga gacggtggtg gtgaaggcag gcgccagcga ctcgaagaga ccgctttcct
     2281 ggctccaggc caggtactcg ggataggacg tcggggccga tgggacggcc ggctgcacgt
     2341 cgcgcaggac caccagccgg tccggatccc tgtacggcag cggccggagc agtacgccat
     2401 cgacgaccga gaagagcacc gtgttgccgc cgatgccgat ggccagggtc aggatcgcga
     2461 cgagggtgat cgccgggctc cggagcagca ttcggaggcc gaagcgcaca tccatcgcca
     2521 gagtcttcat cggaatctcg cgagtgccgg agcaagcccg gtgccacgcc tttcgctcct
     2581 gctcacgctt ccagcgggcg tcggcggcgg gcggttccgg aatgccggcg tcccgggacc
     2641 ggacgatcag gcgctgtcgt cgaaccggcg ggcgatgagg gtcatgcggc ggcggttggc
     2701 gccgagatcg gaccagccga cgcgggaggc ggaacggacg tcgatacgct ggttcgaggc
     2761 atcgagaagg aattcggcat catcgacgaa gccgaagaag cggctccgga actcgacgtg
     2821 gaggtatccg ggccgctcgg cggctgtgcg cgcctgcggc agagaggcca ggagcgtgcg
     2881 caggcgctcc cacgcaatcg cgggtggccc ctcgaagcgg atcggatcga ccgcgtggcg
     2941 cgggtccgcg gcgagcgagg tcacgcagtt cggggagccg ggacacgcgg agagggtccc
     3001 gacgtcggcc gcgcccggcg caggagcgcc ggcgcgcgag cgggcggaga ggatcgcgag
     3061 cgccgcggcc gcgacggcga cacctgcggc gacggcgaag acgagggcca tcgacatccg
     3121 cgccccccgc ctacttccag tccaccggca ggcgcagcgc ccgcccgtcc ctgtcgcggt
     3181 agaggatgta gccgttggtg gcgccgaacc ggtagacgct gcgggtcacc tcgacgtcct
     3241 tgcggcagta ctcctcgatg aggtcgatgc gcccttcctt ccaccactgc agcgactgca
     3301 ggccgtcgcc gcttttcccc accccgagcg tcgcctcccc gacgtccgac agcttcagcc
     3361 ggaatcccag cttcgaatag atcaccgaca gcatgtcgag ggtgcgcacg cggctcagat
     3421 cgcgatcggt gtagccgcgc agcacgccga ggtcgaagcg atcgatgttg tacccgacga
     3481 cccggtcggc ggtgagcagg tcgatc
//
LOCUS       DASRIQ010000519         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2027088, whole genome shotgun sequence.
ACCESSION   DASRIQ010000519 DASRIQ010000000
VERSION     DASRIQ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027088"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VFD06_12710"
     CDS             <1..1158
                     /locus_tag="VFD06_12710"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZN04435.1"
                     /translation="AIGQEDAPDEPHPTALYRNNGDGTFADRSADLGPDTRFGYVKAV
                     VWGDYDNDGRPDLFCSVLSGGNRLFRNAGPPEGAADGAAGWRFTDVTRPAGLADKKDS
                     FPSWFWDFDNDGWLDLLVAGFKFVDMNDIVAFHLGQPTQTAFPSLFRNNHDGTFTDVA
                     HATRIDRIALAMGSNYGDLDNDGWPDCYFGDGEPGFRSLIPNRMFRNDGGRRFQDITH
                     SAGTGHIQKGHGVAMGDLDNDGDLDVFEEMGGWYQDDQAHSALYRNPGHPEHHWVTLR
                     LEGSRTPGASSADRATNRSAIGARIRVRVQDAKGERDVHAIVGTGGSFGGNSLQAEIG
                     LGAARAIREIEVVWPTTGRKDTLRDVTMDRFYRLVEGTGRLEPVTRKKIPL"
     gene            complement(1186..1617)
                     /locus_tag="VFD06_12715"
     CDS             complement(1186..1617)
                     /locus_tag="VFD06_12715"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZN04436.1"
                     /translation="MPTRQSNAVWNGGLKNGSGKVRVGKGVFEGPYTFASRFETGEGT
                     NPEELLAAAHAGCLSMALAAGLERAGTPATMIATRADATLEKVGEAFRITRIKLEVRG
                     TVPGVSPAAFQEAAEKAKEGCPVSNALKGNVAMELDARLAD"
     gene            complement(1617..2333)
                     /gene="pepE"
                     /locus_tag="VFD06_12720"
     CDS             complement(1617..2333)
                     /gene="pepE"
                     /locus_tag="VFD06_12720"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HZN04437.1"
                     /translation="MRLLLLSNSTTFGGTYLGHAREPIQEFFQDIRRLAFVPYALADQ
                     LGYHAKVRDHFKTTFGIEVDRVEEGAGGRTLVERADGLFVGGGNTFRLLDRMQRASLV
                     EPIRARVLEGMPYLGSSAGTGIAAPTLMTTNDMPIVRPASFESLGLVPFQMNCHYLDP
                     DAASRHMGETRETRIREYHEENDTPVVGLREGSWLNVEGTGGAQTARLGGPLHARYFE
                     RGREPRELPPGTDVNAQGGR"
     gene            2473..>3139
                     /locus_tag="VFD06_12725"
     CDS             2473..>3139
                     /locus_tag="VFD06_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN04438.1"
                     /translation="MRTRVLGIVLFAAVGMLLAPAALLHGQPAKRLITVADMNAIRDV
                     SGPDFSPDGQWVAYGVSYVNTKRDKSDSDLYMTSWDGSRTLRLTSSPAKEHTPRFSPD
                     GKWIGFLSKRATKPKADQVWLLPRDGGEAQRLTDLKGGVTDFAFSPDGKKLVVAADDP
                     DPEDLEKEKKDSQEEDEADDGDDDEPTPKPIVINRYQFKVDEEGWLRAGRSHLYLVDL
                     ATRK"
BASE COUNT          492 a         1122 c         1067 g          458 t
ORIGIN      
        1 gccatcggcc aggaggacgc gccggacgag ccgcacccga ccgccctgta ccgcaacaac
       61 ggggacggca ccttcgccga tcgctccgcc gacctcgggc cggacacgcg cttcggctac
      121 gtcaaggcgg tggtgtgggg ggactacgac aacgatggcc ggcccgatct cttctgttcg
      181 gtcctcagcg gcgggaaccg cctcttccgc aacgccggcc ccccggaggg ggccgccgac
      241 ggcgcggccg gctggcgctt caccgacgtc acgcggccgg ccggtctggc ggacaagaag
      301 gactccttcc cgtcctggtt ctgggacttc gacaacgacg gctggctcga tctcctggtc
      361 gccgggttca agttcgtcga catgaacgac atcgtcgcgt tccatctcgg ccagccgacg
      421 cagaccgcgt tcccgagcct gttccgcaac aaccacgacg gcaccttcac cgacgtggcg
      481 cacgcgaccc gcatcgaccg gatcgccctg gcgatgggat ccaactacgg ggacctcgac
      541 aacgacggct ggcccgactg ctacttcggc gacggcgagc cgggcttccg gtcgctcatc
      601 ccgaaccgga tgttccgcaa cgacggcggc cggcgcttcc aggacatcac ccactccgcc
      661 ggcaccggcc acatccagaa ggggcacggc gtcgcgatgg gggacctcga caacgacggc
      721 gacctcgatg tcttcgagga gatggggggc tggtaccagg acgaccaggc ccactccgcc
      781 ctgtaccgta atcccggcca cccggaacac cactgggtga cgctgcggct cgaggggagc
      841 aggacacccg gtgcttcatc cgcggacaga gcgacgaacc gctcggcgat cggcgcccgc
      901 atccgcgtcc gcgtgcagga cgcgaaaggg gagcgcgacg tccacgcgat cgtcggcacc
      961 ggcggttcgt tcggcggcaa cagcctgcag gcggagatcg gcctcggcgc cgcgcgtgcc
     1021 atcagggaga tcgaggtcgt gtggccgacg accgggcgga aggacaccct gcgcgacgtg
     1081 acgatggacc ggttctaccg cctcgtcgag ggaaccggtc gtctcgagcc ggtcacgcgg
     1141 aagaagatcc cgctctgagc aatcggtcag gcgccgccgg gaacctcaat cagcgagccg
     1201 cgcgtccagc tccatcgcga cgttcccctt gagggcgttg ctcaccgggc agccctcctt
     1261 cgccttctcg gccgcctcct ggaaggcggc cgggctgacg ccggggacgg tgccgcggac
     1321 ctccagcttg atgcgggtga tgcggaacgc ctcgccgacc ttttcgaggg tcgcgtcggc
     1381 gcgcgtggcg atcatcgtcg ccggagtccc ggcccgctcc agcccggccg cgagagccat
     1441 gctgaggcag ccggcgtggg ccgccgccag cagctcctcc gggttcgtgc cctcgccggt
     1501 ctcgaagcgc gaggcgaacg tgtaaggccc ctcgaagacc cccttcccga cccgcacctt
     1561 ccccgagccg ttcttcaacc cgccgttcca gaccgcgttc gactgtcgtg tcggcatcat
     1621 cgtcctccct gtgcgttgac gtcggttccc ggtggcagct cgcgcggctc gcggccgcgc
     1681 tcgaagtacc gcgcgtgcag cggcccgccg aggcgggcgg tctgcgcccc gcccgtcccc
     1741 tccacgttca gccatgaccc ttcgcgcagc ccgacgaccg gcgtgtcgtt ctcctcgtgg
     1801 tactcgcgga tgcgggtctc gcgcgtctcc cccatgtggc gcgacgccgc atccgggtcg
     1861 aggtagtggc agttcatctg gaacggcacg aggccgagcg attcgaagga ggccgggcgg
     1921 acgatcggca tgtcgttggt cgtcatgagg gtcggggcgg cgatcccggt gccggcgctg
     1981 gagccgaggt acggcatccc ctcgaggacg cgggcgcgga tcggctcgac gagcgacgcg
     2041 cgctgcatcc tgtcgagaag ccggaaggta ttgccgccgc cgacgaacag gccgtcggcc
     2101 ctctccacga gggtccggcc gccggccccc tcctccacgc ggtccacctc gatcccgaac
     2161 gtcgtcttga agtgatcgcg gaccttggcg tggtagccga gctggtcggc gagggcgtac
     2221 ggcacgaagg cgagccttcg gatgtcttgg aagaactcct ggattggctc gcgcgcgtgc
     2281 ccgaggtagg tcccgccgaa cgtcgtcgag ttgctcagga ggagcagccg caccgtcgtc
     2341 atccctgttg gctcccgtcg gatgtcaggc attctacgcg ctcgtccgag cccggccgaa
     2401 ccgggggctt gccccggcgg gcgacccgcg ccagaatccg cgccgctcca tcattccggg
     2461 ggaacgtcca tcatgcggac cagggtcctg ggaatcgtcc tgttcgcggc cgtggggatg
     2521 ctgctcgcgc cggccgcgct tcttcacggg caaccggcga agcgcctcat cacggtggcc
     2581 gacatgaacg ccatccgcga cgtctccggt ccggacttct cccccgacgg gcagtgggtc
     2641 gcctacggcg tctcctacgt caacacgaag cgcgacaagt ccgacagcga cctctacatg
     2701 acctcgtggg acggcagccg cacgctgcgg ctgaccagca gcccggcgaa ggagcacacg
     2761 ccgcgcttca gccccgacgg gaaatggatc ggctttctct ccaagcgggc gacgaagccg
     2821 aaggcggacc aggtgtggct cctgccgcgc gacggcggcg aggcgcagcg cctgaccgat
     2881 ctgaaggggg gcgtcaccga cttcgccttc tcccccgacg ggaagaagct ggtcgtggcc
     2941 gccgacgatc ccgacccgga ggatctcgag aaggaaaaga aggacagtca ggaggaggat
     3001 gaggcggacg acggggatga tgacgagccg acccccaagc cgatcgtcat caaccgctac
     3061 cagttcaagg tggacgagga gggctggctg cgcgccggcc gcagccacct gtacctcgtg
     3121 gacctcgcga cacgcaaga
//
LOCUS       DASRIQ010000520         6383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_977354, whole genome shotgun sequence.
ACCESSION   DASRIQ010000520 DASRIQ010000000
VERSION     DASRIQ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977354"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFD06_12730"
     CDS             <1..920
                     /locus_tag="VFD06_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZN04439.1"
                     /translation="TIDFRVSIMPSVHGEDAVIRILDKESVNQEFHELRLDVLGFSAD
                     DLKRLRKFIREPYGMVLVTGPTGSGKTTTLYAALSEIRSPEDKIVTIEDPVEYQLPGI
                     TQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDEETAQIAIQSALTGHLVFTTVHA
                     NNVVDVLGRFINMKVEPYNFVSALNCVVAQRLVRIICPKCRHAVRYSAETLQESGIDP
                     VHADKTFFEGRGCIDCNGTGYMGRMAIAEILDMSDRVRQLILDRRSAAEIKKAAKEDG
                     MTFMREAAVQRVLEGRTTLREINKATFVE"
     gene            958..1986
                     /locus_tag="VFD06_12735"
     CDS             958..1986
                     /locus_tag="VFD06_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04440.1"
                     /translation="MKDSLERLRVLFDGTSLDSPLPPPFPPAAVEIAPERVSGVRVVR
                     ERRGKRMVVKAAAWRDLPKGAVEATLVRPNVRDEAAVGAALDEVLKAVAPGEHRVSLL
                     LPDAAARVSLLTFPALPGTRRELEELVRFRMTKALPFKADEAAMDLVPLGAAVGSGAM
                     PGSGAVMAVFLHRPILDQYEGLLTARGYWPGLVGLSTLELFNLYRSMLGSSLHEKDLM
                     LLNLTHRDLSLLIASGGKVIFYRCKNLPTGVDGLPDPQAVRREIYTSLAFYQEKLLGR
                     GLGRVWLRAAGMDPEPVAEAVREEAGTAVEVLTLSQVLHLHEATGLEESGAALLAPAA
                     GAVAGRRA"
     gene            1983..3413
                     /locus_tag="VFD06_12740"
     CDS             1983..3413
                     /locus_tag="VFD06_12740"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HZN04441.1"
                     /translation="MKPLPLNLASRPFRNNILVGTLLTVLGVGLVGGTAFNLYVYLSY
                     GAAYATLQHAQAEDRARIAVLQKQERDLAAQIKTRDFRSAYSRGKMASDLIRKSAFSW
                     TQLFNTLETVIPANVVLAAIRPNISSDGIVLRIEGVAKSHEALLTLEEKLQKHDAFKR
                     VAPISERKLNPNLPDITFLLTTDYIPPSQQPPVVTAANEAGGAAAAPATGGPAAAVTS
                     EAAAAPSTAPPPGEAARPPVVPPADGTKAASGTLVGRDGRPKSESGSVIAPGGLLIAE
                     AVAAPAPSKDKKGSRKPAATTTSTPAPASGATGAKASAPTAPQAAGNKGAPANAAPPV
                     AIPPAQRPIATPRGYDPNVARTMPSQLQTTASRPKATPPARAERLDTPLTFVGRPAGE
                     VYRTLAAAHGVRIDLDPTVDPSAPVTLNLQGRRLEEALLLLSGVLHTQMTRAGEGVYR
                     ITSTEVRAPAGDKPPVEETIVEEGQR"
     gene            3410..4117
                     /locus_tag="VFD06_12745"
     CDS             3410..4117
                     /locus_tag="VFD06_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04442.1"
                     /translation="MKPATNGNGSVKGTRRRRRFDLRQDLWRVGAILGALVLLNVGFY
                     LFLNMPRLKALSDLATGSDTVRRTLVDVSRSCQSMQELIARYDSETTRLDAFYTERIG
                     TQMERMTAIQQALRSIAAEFRVDPESMDYNTSEVEGSDLVRFQITIPLNGGYPNLRHF
                     INRVETAKYLAVIDSIELAGSRDGGAMLSLTIRISTYFREPDLGRRLEAGEGDGAART
                     DGDAPPPRRRRSGGPAT"
     gene            4114..4794
                     /locus_tag="VFD06_12750"
     CDS             4114..4794
                     /locus_tag="VFD06_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04443.1"
                     /translation="MIPEALMKRKELVLGVLVILLAAVAWRTLSGEGMLVPGIGAAAT
                     PGRTKVDLAPLKIFPVDWASLDAPRPAYDPNGRNIFQYGVIVPPPPPPLTAAEKAAIA
                     KAQAEAKKAAEAQAEANRLAAERAAVDAAAAADRERIAAANRPPTPPPPPEPPPIPYK
                     FIGYIGPVNAKMAVLYDGTDTIFVKSGERLPKGIKVLEIGYESIKFGFTEPQFKDQSR
                     TLPMSSSY"
     gene            4805..>6383
                     /locus_tag="VFD06_12755"
     CDS             4805..>6383
                     /locus_tag="VFD06_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZN04444.1"
                     /translation="MRRSLDRTVRRQIAGWVLVLFITQIGCATTGSYKSGMKAYKARR
                     YDQAVVEFSQALAKKPDSTRYKVALARARISSAQEHFQKGDKYHKAGLLEEAIAEFQQ
                     AVYLDSSHQFAAAELQKAVGEWQRKQEEQSWDLERAKKRAKTSPGREAPRLSASANIP
                     IVLRFKDENVKKIYDALSKATGVNFLYDERLPLDKKVSVDIADVTFEQAMDQLMTMNK
                     HFYKILDETSIFIAEDTPQKHKELDDLVIQTFYLSNADVKDVQVLLRTLLDARQIAQN
                     DRLNAITIRDTPERVQVAQKIIEANDKAKSELIIDIELLEVNRNILQNLGIDLFGVGA
                     SGGTGKTVNLSFGGGSSLPLNNLDLLKQAGSWLVGPIPGVVVNFLKTDADAQVIAKPQ
                     LRVSEGAKATVRIGDRIPIPNTTFNSAPTVGGTVGVPITSFTYQNVGINIDLEPRVHH
                     NREITLKGKIEISSLAGQVSAGGGLTQPIIGTREIESTIRLRDNETNILAGLIREEER
                     KSLSGVPGLSDIPVLKRL"
BASE COUNT         1083 a         2271 c         2184 g          845 t
ORIGIN      
        1 gcaccatcga cttccgcgtc tctatcatgc ccagcgtcca cggcgaggac gcggtcatcc
       61 gcatcctgga caaggagtcg gtgaaccagg agttccacga gctgcgactg gacgtgctgg
      121 gcttctccgc cgacgacctc aagcgcctcc gcaagttcat ccgcgagccg tacggcatgg
      181 tgctggtgac cggtccgacg ggatcgggga agacgacgac gctgtacgcc gccctctccg
      241 agatccgctc gccggaggac aagatcgtga cgatcgagga cccggtcgag taccagctcc
      301 cggggatcac gcagatcccg gtgaacgaga agaaggggct gaccttcgcc cgcggcttgc
      361 ggtcgatcct gcggcacgac ccggacaaga tcatggtcgg ggagatccgc gacgaggaga
      421 cggcgcagat cgccatccag tcggcgctga ccggccacct cgtcttcacg accgtccacg
      481 ccaacaacgt cgtggacgtg ctcggccgct tcatcaacat gaaggtcgag ccctacaact
      541 tcgtctcggc gctgaactgc gtcgtggcgc agcgcctggt ccggatcatc tgcccgaagt
      601 gccgccacgc ggtgcgctac tccgcggaga ccctccagga gtccggcatc gacccggtgc
      661 acgccgacaa gaccttcttc gaggggcgcg gctgcatcga ctgcaacggc accggctaca
      721 tggggcggat ggcgatcgcc gagatcctcg acatgagcga ccgggtccgg cagctcatcc
      781 tcgatcgccg ctcggcggcg gagatcaaga aggccgccaa ggaggacggc atgaccttca
      841 tgcgcgaggc ggcggtgcag cgggtcctcg aaggccggac gacgctgcgc gagatcaaca
      901 aggcgacgtt cgtcgaataa gggcacgggg agcggggagc acggaggcac gatgggcttg
      961 aaggattcac tggagcggct gcgcgtgctg ttcgacggca cgtcgctcga ttccccgctc
     1021 ccgccgccgt tcccgccggc cgcggtcgag atcgcgccgg agcgcgtgtc gggcgtgcgc
     1081 gtggtgcgcg agcgccgcgg caagcggatg gtggtgaagg cggcggcgtg gcgcgacctg
     1141 ccgaaagggg cggtcgaggc gacgctcgtc cgcccgaacg tgagggacga ggcggcggtc
     1201 ggcgcggcgc tggacgaggt gctgaaggcg gtcgcccccg gcgagcacag ggtgtcgctg
     1261 ctcctgccgg acgccgccgc gcgggtgtcg ctcctgacct tcccggccct gccgggaacg
     1321 cgccgcgagc tggaggagct ggtccgcttc cggatgacca aggcgctgcc gttcaaggcg
     1381 gacgaggcgg cgatggatct cgtgccgctc ggggcggcgg tcggctccgg ggcgatgccg
     1441 gggtcggggg cggtgatggc ggtcttcctg caccgcccga tcctcgacca gtacgaaggg
     1501 ctcctgaccg cgcgcggcta ctggcccggg ctcgtggggc tgtccacgct cgagctgttc
     1561 aatctctacc gctcgatgct gggctcgagt ctccatgaga aggacctgat gctcctgaac
     1621 ctgacgcacc gggacctgtc gctgctcatc gccagcggcg ggaaggtgat cttctaccgc
     1681 tgcaagaacc tgccgaccgg tgtcgacggg ctcccggacc cgcaggcggt gcggcgggag
     1741 atctacacgt cgctcgcctt ctaccaggag aagctcctcg gccgcgggct cggccgcgtc
     1801 tggctgcgcg ccgccggcat ggatccggag ccggtggccg aggcggtgcg cgaggaggcg
     1861 gggaccgcgg tcgaggtcct gaccctgtcg caggtgctgc acctccacga ggcgaccggc
     1921 ctcgaggaat cgggggcggc gctgctcgcc ccggccgccg gcgccgtggc ggggaggcgt
     1981 gcgtgaagcc gctgccgctc aacctcgcga gccgcccgtt ccgcaacaac atcctcgtcg
     2041 ggacgctcct cacggtcctc ggcgtcggcc tggtcggcgg caccgccttc aacctgtacg
     2101 tctacctgtc ctacggcgcg gcctacgcca cgctgcagca cgcccaggcg gaggaccggg
     2161 cccgcatcgc cgtgctgcag aagcaggagc gggatctcgc cgcgcagatc aagacgcgcg
     2221 acttccggag cgcctacagc cgcggcaaga tggcgagcga tctcatccgc aagagcgcct
     2281 tctcctggac gcagctgttc aacaccctcg agacggtgat cccggcgaac gtcgtcctgg
     2341 ccgccatccg gccgaacatc tcgtcggacg ggatcgtgct gcgcatcgag ggggtggcga
     2401 agagccacga agccctcctg acgctggagg agaagctgca gaagcacgac gccttcaagc
     2461 gggtggcgcc gatcagcgag cggaagctca acccgaacct gccggacatc accttcctcc
     2521 tcaccaccga ctacattccg ccgagtcagc agccgcccgt ggtcaccgcc gcgaacgagg
     2581 cgggcggcgc cgcggccgcg ccggcgacgg gcgggcccgc ggccgccgtg acgtcggaag
     2641 cggccgcggc cccctcgacg gccccccctc cgggcgaagc cgccaggccg ccggtcgttc
     2701 cgcccgcgga cgggacgaag gccgcctccg ggacgctcgt cgggcgcgac ggacggccga
     2761 agtccgagtc cggcagcgtg atcgccccgg gcgggctgct catcgccgaa gccgtcgccg
     2821 cgccggctcc gtccaaggac aagaagggat cccgcaagcc ggccgcgacc accacgtcca
     2881 cgcccgcccc ggcgtcgggt gcgacgggcg cgaaggcgtc cgcgccgacc gcgccgcagg
     2941 cggcgggcaa caagggcgcg cccgcgaacg ccgcgccccc agtcgccatt ccgccggcgc
     3001 agcggccgat cgccacgccg cgcggctacg acccgaacgt ggcacggacc atgccgagcc
     3061 agctgcagac caccgccagc cggccgaagg cgaccccgcc ggcgcgcgcc gagcggctcg
     3121 acacgccgct gacgttcgtc ggccgccccg cgggcgaggt gtaccgcacc ctcgccgcgg
     3181 cgcacggcgt gcgcatcgac ctcgatccga ccgtggaccc ctccgccccg gtcaccctca
     3241 acctccaggg ccggcgcctc gaggaggcgc tgctgctcct ctccggggtg ctgcacacgc
     3301 agatgacccg cgccggtgag ggggtctacc gcatcacctc gacggaggtg agagcccccg
     3361 ccggcgacaa gccgccggtc gaagagacga tcgtcgagga ggggcagcga tgaagccggc
     3421 gacgaacggc aacggcagtg tcaagggaac ccggcgccgc cgccgcttcg acctgcgcca
     3481 ggacctgtgg cgcgtcggcg cgatcctcgg ggcgctcgtg ctcctcaacg tcggcttcta
     3541 cctcttcctc aacatgccgc gcctcaaggc gctctccgac ctcgcgaccg gcagcgacac
     3601 ggtccgccgg acgctcgtgg acgtgagccg ctcctgccag tcgatgcagg agctgatcgc
     3661 ccgctacgac agcgagacga cccgcctcga cgccttctac accgagcgga tcggaaccca
     3721 gatggagcgg atgaccgcca tccagcaggc gctgcgctcc atcgccgccg agttccgcgt
     3781 cgacccggag tcgatggact acaacaccag cgaggtggag gggagcgacc tcgtccgctt
     3841 ccagatcacc atcccgctca acggcggcta cccgaacctt cggcacttca tcaaccgggt
     3901 ggagacggcc aagtacctcg ccgtcatcga ctcgatcgag ctcgccgggt cgcgcgacgg
     3961 cggcgccatg ctcagcctga cgatccggat ctcgacctac ttccgcgagc cggatctcgg
     4021 ccggcggctc gaggcgggcg aaggggacgg cgccgccagg acggacgggg acgcgccgcc
     4081 gccgcgccgc cgccgctccg gagggccggc cacgtgatcc ccgaggcgct catgaagcgc
     4141 aaggagctgg tcctcggcgt gctggtgatc ctcctggcgg ccgtcgcatg gcggaccctg
     4201 tcgggggaag ggatgctcgt ccccggcatc ggcgccgccg cgacccccgg gcggacgaag
     4261 gtcgatctcg cgccactcaa gatcttcccg gtggactggg cgtcgctcga cgcgccgcgc
     4321 cccgcgtacg atccgaacgg ccgcaacatc ttccagtacg gcgtcatcgt gccgccccct
     4381 cctccgccat tgaccgcggc ggagaaggcg gccatcgcca aggcgcaggc ggaggcgaag
     4441 aaggcggccg aggcgcaggc ggaggcgaac cggctcgcgg cggagcgcgc ggccgtcgac
     4501 gccgccgcgg ccgccgaccg ggagcgcatc gccgcggcca accgtccgcc gacccccccc
     4561 ccgccgccgg agccgccccc catcccctac aagttcatcg ggtacatcgg cccggtgaac
     4621 gccaagatgg cggtgctgta cgacggcacc gacacgatct tcgtgaaatc gggggagcgt
     4681 ctcccgaagg ggatcaaggt tctcgagatc ggttacgagt cgatcaagtt cgggttcacc
     4741 gagccgcagt tcaaggatca gtcacgtacg ctgcccatga gcagctccta ctaggagaca
     4801 ccgtgtgagg cggagcctcg atcggacggt acgaagacag atcgcagggt gggtgctcgt
     4861 cctgttcatc acgcagatcg gctgcgccac gaccggctcc tacaagtcgg ggatgaaggc
     4921 gtacaaggcg cggaggtacg accaggcggt cgtcgaattc tcgcaggccc tcgccaagaa
     4981 gccggacagc acccgctaca aggtcgccct cgcccgcgcc cgcatctcct cggcgcagga
     5041 gcacttccag aagggggaca agtaccacaa ggccgggctc ctcgaggagg cgatcgccga
     5101 gttccagcag gcggtctacc tcgactcgtc gcaccagttc gccgccgccg agctgcagaa
     5161 ggcagtgggc gagtggcagc gaaagcagga ggagcagtcg tgggatctcg agagggcgaa
     5221 gaagcgggcc aagacctcgc cggggcgcga ggcgccgcgc ctcagcgcct ccgccaacat
     5281 cccgatcgtc ctgcgcttca aggacgagaa cgtcaagaag atctacgacg ccctgtcgaa
     5341 ggcgaccggc gtcaacttcc tctacgacga acggctgccc ctcgacaaga aggtctcggt
     5401 ggacatcgcc gacgtgacgt tcgagcaggc gatggaccag ctgatgacca tgaacaagca
     5461 cttctacaag atcctcgacg agacctcgat cttcatcgcc gaggacacgc cgcagaagca
     5521 caaggagctg gacgatctcg tcatccagac cttctacctg tcgaacgccg acgtcaagga
     5581 cgtgcaggtg ctgctccgga cgctcctcga cgcgcggcag atcgcccaga acgatcgcct
     5641 gaacgccatc acaatccgcg acacgccgga gcgcgtgcag gtggcgcaga agatcatcga
     5701 ggcgaacgac aaggcgaagt ccgagctgat catcgacatc gagcttctgg aggtgaaccg
     5761 caacatcctc cagaacctcg gcatcgatct cttcggcgtc ggggcctccg gcggcaccgg
     5821 caagacggtc aacctgtcct tcggcggcgg ctccagcctg ccgctcaaca acctcgacct
     5881 cctcaagcag gcggggtcgt ggctcgtcgg gccgatcccg ggcgtggtcg tcaacttcct
     5941 caagaccgac gccgacgcgc aggtgatcgc caagccgcaa ttgcgtgtca gcgaaggggc
     6001 gaaggcgacg gtgcgcatcg gcgaccgcat cccgatcccg aacacgacct tcaactcggc
     6061 gccgaccgtc ggcgggaccg tcggggtgcc gatcacctcg ttcacctacc agaacgtcgg
     6121 catcaacatc gacctggagc cgcgggtgca ccacaaccgc gagatcaccc tgaaggggaa
     6181 gatcgagatc tcctcgctcg ccgggcaggt gtcggcaggc ggcgggctga cgcagccgat
     6241 catcgggacg cgcgagatcg agagcaccat ccggctgcgg gacaacgaga ccaacatcct
     6301 cgccggcctc atccgcgagg aggagcgcaa gtcgctctcg ggggtgccgg ggctgtcgga
     6361 catcccggtg ctcaagcggc tct
//
LOCUS       DASRIQ010000521        12224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_740945, whole genome shotgun sequence.
ACCESSION   DASRIQ010000521 DASRIQ010000000
VERSION     DASRIQ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12224
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740945"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="VFD06_12760"
     CDS             <1..1588
                     /locus_tag="VFD06_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04445.1"
                     /translation="CQADGRCGGSPRVCTDPYDCTIDGCDPAHGCTFVTDCDDGSECT
                     SDFCGSSGCGHLPIGGHCFPTNRCIDDGLSHWSCIDNHLVCTGPPRICNDDTPCTIDS
                     CDPGRGCVYQPVSCDDGNPCTFDLPCQFMTGCYPDHPFQPGPCDDNDPCTTSDTCVPP
                     DLNHFRAYCRGTSSCDDGNPCTDDICDAAAPGGCRHVNNTDACSDGVTCTRGDLCVEG
                     TCRPGEPRSADCNDGDFCSDDVCDPIRGCVSTPRCDDGNPCTGNGCTQTACFFTPISG
                     PACGDACVIGGVCVNGGCVGTPVSCDDSSACTTDACDPQSGCRNDPIDCNDGNVCTLD
                     ECDPANGCRHLPALDCDDGNACTLDACDALSGCQSRFDPALPDLDGDGVPDLCDGCPA
                     VPNADQADGDGDGVGDACDVCPTLPDPGQDPSICVQQVTDLTLELGTGGPGKGAGLVS
                     WRTTHEVSLAGFDIVSIDARGRRTLLTPVRVPCTACATGEGAGYSVLVQKHRSSRDVY
                     VEMIGSDGGLLGRFGPAIRR"
     gene            1827..2384
                     /locus_tag="VFD06_12765"
     CDS             1827..2384
                     /locus_tag="VFD06_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04446.1"
                     /translation="MIVFRAVLIALLAWWTARLGSGAQQGCFLDLVNLAFHEAGHLFF
                     SPFGTTMHFLGGTLGQLLVPAGLAVYFVKARQEPFAAAACAWWLGESLVNISVYMSDA
                     RDLALPLVGGGDHDWNNLFYAFGLLGQESVAAVSAWTHRCGTIVMVLGLAWLATFLLT
                     GESRRRFESAIEARIPILRRLVSAE"
     gene            complement(2425..5730)
                     /locus_tag="VFD06_12770"
     CDS             complement(2425..5730)
                     /locus_tag="VFD06_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04447.1"
                     /translation="MLRTFALIPLIALGLFIPASARSQTSGKGPPQSRAGTGLAAAAA
                     LPEGLRSATPETPCWASFAARRADLDDPAALVALLDEARGRSLKTLVRLEEADREAGT
                     GWTDRLLGLATALGDRVDAYQLLGEEAGRMPAKDYAFILKNARVSIRAAGSNAVILGP
                     PFDLLDEAWPEQLFAEDAAPYLDVVSASHVTALERVMAVRDKGHPRAPVWVTDSALPE
                     EGAIPAAIAGYLEAMSAGAEVVIFREAQAPPESTPAAPAPLQPAPEPVASETPEPETM
                     AEPVTSETPEPGTASEPATAATKPTPPALGEVLTFLRSQFPPGLRPAAKGALPFNPAA
                     AVAGSAHGAQTGEENGSGQAAPGESAPAAGQDSKVDLVVLPFFDEKTRDGLAAFRAGE
                     EDETPPPAVRLPLRAPVESLELITPERMQERTLSGAAGPGATVILPLRPGYRLLRYRL
                     ALEAIPVKESTGVDASAELTAEEIIAFERERSGVQKVRVDHYEAKAVINLHWRLASIG
                     ETLDLASENRLYVHDGKEDYEQLSLFVNGARWRSKEPPKLPFVQPDKVGEMPLDIALD
                     EQYRYTLEGREKIDGRECYVLSFEPIDPSQSLYHGKVYIDRILFTRVRMEVVQTALTP
                     PVRSSEIVFSYGPVPSDVGEVWLPVNIDGQMVYELLGYTLAIEREHIYTDFRINEPGI
                     DERVAQAFDSGRPVFRDTDKGLQRVRIVDGEETFESLDRPRNTLLVFGFSAGEEGDLS
                     FPFAGVNFFDFNFLDSGTQFNLAVAGPFVDISWNQPNVLAESVRKPWSVAVQSSLTAI
                     ELEDQLATDAGTLSQDRVDILSQEFRATLGIPMGDFLRWSLQGRALYQNFDRQEKTST
                     SFVLPPTNTQGVFNLRLEFSRLGYQVAPWAEWGTRSDWGPWGRCADVPFEECDVPGAR
                     FSEDDRDFTRLGIDIRKSYYFGIFHKITLGLSGFEGRSLDRFSRFELGDFRSARVRGF
                     NGSGIHFDRGVVAEAAYSFGISKALRAEVSIEEGIIQSEDDFLDGYERVIGSGLNLEF
                     SGPWGTFVTVRIGAALTSTIPDKGEGADLRVVFFKTWDKWSPKGGKGTPPSQPPPAPG
                     PAAPDPVPIDPDR"
     gene            5891..7525
                     /locus_tag="VFD06_12775"
     CDS             5891..7525
                     /locus_tag="VFD06_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HZN04448.1"
                     /translation="MQYSVSPDGEKFPLPTPEEYQKEFARLESLTAAERAKGREIVVV
                     VGVGFVGAVMAAVVADSSDKKTGQAGKFVIGMQRPSTRSYWKIPLLNRGLSPVKSEDP
                     EVDVLIQRCVKERKNLVATYTEDALKLADCVVVDVQCDYLKESLGDLKTGHADMEALE
                     KSFVSIGRLIQPNCLVLIETTVAPGTTEFIAYPILKKEFEKRGITTAPLLAHSYERVM
                     PGRDYVRSIRDFWRVCAGVNPESRKRVVAFLSEVLNVDTFPLTVLDRPLESETCKIVE
                     NSYRATILAFLDEWSRFAERNGVDLVKVIQAIKVRPTHDNIIFPGPGIGGYCLPKDGG
                     LGIWANRHLMGWEEETFKITPLAININDTRALHVGTLTRDALRNMGKVVAGSHLVLLG
                     AAYREDVGDTRYSGSELIVRKLAEMGGDMAVHDPYVMHWWEFEEQETYPREGYSWSRF
                     FRNQEGLKNLRMQQDLGAALKGADAVILAVRHKPYLELQPEDVVKQAGRPLAVVDCFG
                     ILDDAKIRRYFELGCEVKGLGRGHIQRIKEDVRSKR"
     gene            complement(7550..9226)
                     /locus_tag="VFD06_12780"
     CDS             complement(7550..9226)
                     /locus_tag="VFD06_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN04449.1"
                     /translation="MTSGPARSRVATLAAILDRWIPSLVAVFLFYTPFLAALGTERNR
                     YFLFWGRKDTLAILLSILLLSGTAALAGLAIDRTAIRILRGLKDVVFLTFLLLGILAN
                     LLPLLHIEALLEFARAHRGAAGVLLDIASGILAIAWVLGVWLRRERMIRIARGATFVF
                     SPLVLLLLGPALFWRSWDYTRDPIPAASGEGENGAPVYFLVFDEWSYQRMVEGHEVRP
                     EFANVRAFQEHALFFRTARAPASSTHVSLPRILFQSRDDEALPYVFIDRKDPEEPSGP
                     RPEHITDNLAPSGAPESLLGLARRNRYTTYLVGFYLPYPHIMGNEADVDRAYSNYPKG
                     DTLVERMVVTTLGAPQYWFMPGISGLWKGIWARVFSRHWVRLNHRVRDDLFAIVGTAP
                     RRALVFVHFPAPHAPFVFNPDGSYRGPFPIHSGATADVDSDIMAGTPEDYHRQMLYVD
                     RLVGEVTERLKRAGDYDRALIIMTADHSWREDPAIAPGDEHRAKRHVPLLIKLPGQTT
                     PRVLDTDVALDELRPLIAGAMRGEVGAGSVAAAETLFRAATPQTAAAVSR"
     gene            complement(9234..10667)
                     /locus_tag="VFD06_12785"
     CDS             complement(9234..10667)
                     /locus_tag="VFD06_12785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN04450.1"
                     /translation="MSEAATGSQRDAASFRDPAGYVFRHAGRVFRALDARSAADLLDL
                     LEMGSLQRLVADRLVVPTSFVASSPLLDRLAIEHAGAARFLEHDAIDPITYPYCWTVS
                     MLADAAALTLDVQLRLLETGHSLKDATAYNVQFARARPLFIDLASIERPPRLDIWVAL
                     GQFQQMFTYPLLLARYRGWDLRSYFLPSLGGRTAAQVGESFRGLSRLRPGLLLDLTLP
                     LWLDRRSGEDGPGREVFDRPNPNSAPQAMNLRRLRKKVLALAAGYRPQGVWARYTQEC
                     SYVDAATAAKKRIVAAMLDRVKPRRVLDLGCNTGVYSLLAAEAGSEVIAADADHDAVE
                     MLYRRVRGQDKPITPIVVDLCNPSPAIGFRNLERPRLVERIGADAVLALALLHHLHVS
                     GNLPLEAIASLFDDLTRDALVLEFVPTDDVMFRRLTRLRRETFEGMTLERCLAAFAPR
                     FDLVAREPVPEGGRVLLLFRKRRAVAA"
     gene            complement(10673..10879)
                     /locus_tag="VFD06_12790"
     CDS             complement(10673..10879)
                     /locus_tag="VFD06_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04451.1"
                     /translation="MEWHVRLAYIDPVSGSILIQTLVAAVAGGAAFFRKSLWALIKRP
                     AAPLSSPGPTPPTATPAEKEPKVS"
     gene            complement(10964..12082)
                     /locus_tag="VFD06_12795"
     CDS             complement(10964..12082)
                     /locus_tag="VFD06_12795"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HZN04452.1"
                     /translation="MRFAFNPFFRAGVVTPSGAQRDVLAFHRSLPGYEPTPLHSLPTA
                     ARLLGIGTLLVKDESRRFGIKAFKALGASWALHALVEERRGAGEAPPETVATATDGNH
                     GRAVAWAARRLGLQAVVFIPAHAARARIEAIRGEGARVVTVEGTYDDTVRRAAEEAAR
                     NSWQVIADTGYEGYLDIPRKVAEGYSTLFQETDAQIEERLLPQPDLVMLQGGVGALAA
                     AAVDHYRQRRTPPRLVVVEPVEADCLLESILSPDGQPRPGRGRQDSIMAGLNCGLPSL
                     AAWPTLRRGIDLFLSIEDRYAEEAVRLLWRGGGTAGEPPIEAGESGGAGVACLIALKQ
                     ADALLAATERLGLGPSTCVLALNTEGATDPEGLRSILP"
BASE COUNT         1881 a         4231 c         4307 g         1805 t
ORIGIN      
        1 ctgccaggcg gacggccggt gcggcggctc tccccgagtg tgcaccgacc cgtatgactg
       61 cacgatcgac gggtgcgatc ccgcgcacgg gtgcaccttc gtcaccgact gcgacgacgg
      121 cagcgagtgc accagcgact tctgcggatc gagcggctgc ggccacctac cgatcggcgg
      181 ccactgcttc ccgaccaacc gctgcatcga cgacgggctc tctcactgga gctgcatcga
      241 caaccacctc gtctgcaccg ggccgccgcg catttgcaac gacgacaccc cctgcacgat
      301 cgattcctgc gatcccggcc gcggatgcgt ataccagccg gtgagctgcg acgacggcaa
      361 tccgtgcacc ttcgacctcc cgtgccagtt catgaccggc tgctatcccg accatccgtt
      421 ccagccgggt ccgtgcgacg acaacgaccc ctgcaccacc agcgacacct gcgtcccgcc
      481 cgacctcaac catttcaggg cctactgcag gggcacgtcg tcgtgcgacg acggcaaccc
      541 gtgcaccgat gacatctgcg acgcggcggc gcccggaggc tgccgccacg tgaacaacac
      601 ggacgcctgc tccgatggcg tcacctgcac ccgcggggac ctctgcgtcg aagggacctg
      661 ccgacctggt gagccgagat ccgcagactg caatgatggc gacttctgct cggacgacgt
      721 ctgcgacccg atccggggtt gtgtgtcgac gccgcggtgc gacgatggca acccctgcac
      781 cggcaacggc tgcacgcaga ccgcgtgctt cttcacgccg atctcggggc cggcctgcgg
      841 agacgcttgc gtcatcgggg gtgtgtgcgt caatggcggc tgcgtcggga cgccggtctc
      901 ctgcgacgac tccagtgcct gcacgaccga cgcgtgcgat ccgcaatccg ggtgccgcaa
      961 cgatccgatc gactgcaacg acggcaacgt ctgcactctg gacgagtgcg atccggccaa
     1021 cggctgccgg cacctgccgg cgctggactg cgacgacggg aatgcctgca ccctcgacgc
     1081 gtgcgatgcg ctttcggggt gccaatcccg gttcgatccg gcccttccgg atctcgacgg
     1141 cgacggcgtc ccggacctgt gcgacggctg tcccgcggtt cccaacgccg atcaggccga
     1201 cggcgacggc gacggcgtcg gggacgcctg cgacgtctgt ccgacgcttc ccgacccggg
     1261 ccaggatcct tcgatctgcg tgcagcaggt caccgatctc acgctggagc tcggcaccgg
     1321 aggtcccggc aagggggccg ggctcgtttc gtggcggacc acgcacgagg tttcgctcgc
     1381 gggcttcgat atcgtatcca tcgatgcgcg cggccgacgg acgctgctga cgcccgtgcg
     1441 ggtgccgtgc accgcctgcg cgacgggaga gggggccggg tacagcgtcc tcgttcagaa
     1501 gcatcgcagc agccgggacg tctatgtcga gatgatcggg tcggacgggg ggctcctggg
     1561 gcggttcgga ccggccatcc gccggtaggc cgaaagggta gacggaactt caagggcggg
     1621 gccgccaggc tccgccctct ctttttgtct tcggtcgccg cgcgccgcgc gccggtgtcg
     1681 cgcccttggc ggtgacgttc accgacctct cgacgaccaa cacgtcgcgt gggcctggga
     1741 cttcggccac gccgcgcgac ccgctgatcg cctcctgccc ttgacggccc ggacgccggc
     1801 cgtatctttg agccgcgggg gactccgtga tcgtctttcg cgccgtgctc atcgccctgc
     1861 tcgcctggtg gaccgcccgg ctcgggtcgg gggcgcagca gggatgtttc ctcgacctcg
     1921 tgaatctggc gttccacgag gcggggcacc tgttcttctc gccgttcggc acgacgatgc
     1981 acttcctcgg cgggacgctg ggccagctcc tggtgccggc cggcctcgcg gtctatttcg
     2041 tgaaggcccg gcaggagccg ttcgccgccg ccgcgtgcgc ctggtggctc ggcgagagcc
     2101 tcgtgaacat ctcggtgtac atgtcggacg cgcgcgatct cgcgctgccg ctggtcggcg
     2161 gcggcgacca cgactggaac aacctcttct acgccttcgg cctgctcggc caggagagcg
     2221 tcgccgccgt ctccgcctgg acgcaccgtt gcgggacgat cgtgatggtt ctcggcctcg
     2281 cgtggctcgc gacgttcctg ctgaccggcg agtcgcgccg gcggttcgag tcggccatcg
     2341 aggcccgcat cccgatcctg cgccgcctcg tctccgcgga atagcgcgtg cgcgggattc
     2401 ccggcggcac gcaggtgcct cggatcagcg atccggatcg attggcacgg ggtccggcgc
     2461 cgccgggccg ggggccggag gcggctgcga cggcggcgtc cccttgccgc ccttggggct
     2521 ccacttgtcc caggtcttga agaagacgac gcgcaggtcg gcgccttccc ccttgtcggg
     2581 aatggtcgag gtcagcgcgg cgccgatgcg cacggtcacg aacgtccccc aggggccgga
     2641 gaactccagg ttcaagccgc tgccgatgac ccgctcgtag ccgtcgagga agtcgtcctc
     2701 gctctggatg atcccctcct cgatggagac ctcggcgcgc agcgccttgc tgatcccgaa
     2761 cgagtaggcc gcctcagcga cgacgccacg gtcgaagtgg atgccggagc cgttgaagcc
     2821 ccgcacccgc gccgaacgga aatcccccag ctcgaagcgc gagaagcggt cgaggctgcg
     2881 cccctcgaag ccggacagac cgagggtgat cttgtggaaa atcccgaagt agtaagactt
     2941 gcggatgtcg atgccgaggc gcgtgaagtc gcgatcgtcc tcgctgaaac gcgcgccggg
     3001 gacatcgcat tcctcgaacg gcacgtcggc gcagcgcccc cacggccccc agtcgctgcg
     3061 cgtcccccat tccgcccacg gcgcgacctg gtagccgagg cgggagaact ccagccggag
     3121 gttgaagacc ccctgggtgt tggtcggcgg caggacgaac gaggtgctgg tcttctcctg
     3181 ccgatcgaag ttctggtaca gggcgcgccc ctggagcgac cagcgcagga agtcccccat
     3241 cgggatgccg agggtggcgc ggaattcctg gctcaggatg tcgacgcggt cctgcgagag
     3301 cgtgccggcg tccgtcgcga gctggtcctc cagctcgatc gccgtgagcg acgactgcac
     3361 cgccaccgac cagggcttcc ggacgctctc ggcgagcacg ttcggctggt tccatgagat
     3421 gtccacgaag ggtccggcga ccgccaggtt gaactgggtt ccggagtcca ggaagttgaa
     3481 atcgaagaag ttgactcccg cgaacgggaa cgacagatcc ccctcttccc ccgccgagaa
     3541 cccgaagacg agcagggtgt tgcgcggccg gtcgaggctc tcgaatgtct cctcgccgtc
     3601 gacgatgcgc actcgctgca gccccttgtc ggtgtcgcgg aacaccggcc ggcccgaatc
     3661 gaacgcctgc gccacccgct cgtcgatccc cggctcgttg atccggaagt cggtgtagat
     3721 gtgctcccgt tcgatggcga gcgtgtagcc caggagctcg tacaccatct gcccgtcgat
     3781 gttcaccggc agccagacct cccccacgtc gctcggcacc ggcccgtagc tgaaaacgat
     3841 ttcgctcgag cgcacggggg gggtcagcgc cgtctgcacc acctccatcc ggacgcgggt
     3901 gaagaggatt cggtcgatgt acaccttgcc gtggtagaga ctctgcgacg gatcgatcgg
     3961 ctcgaacgac aggacgtagc actcacgccc gtcgatcttc tcgcgtccct cgagggtgta
     4021 gcggtattgc tcgtcgagcg cgatgtcgag cggcatctct ccgaccttgt ccggctgaac
     4081 gaacggcagc ttgggcggct ccttgctgcg ccagcgggcg ccgttgacga acagggagag
     4141 ctgttcgtag tcctccttgc cgtcgtggac gtacagccgg ttctcgctcg ccagatcgag
     4201 cgtctcgccg atcgacgcga gacgccagtg caagttgatg accgccttgg cctcgtagtg
     4261 atcgacgcgg accttctgca ccccgctgcg ctcccgctcg aaggcgatga tctcctccgc
     4321 cgtcagctcg gcgctggcgt cgacgccggt cgactccttc accgggatcg cctcgagggc
     4381 cagccggtag cgcagcagcc ggtaccccgg ccgcagcggc aggatgaccg tcgcccccgg
     4441 ccccgccgcc ccggacagcg tccgctcctg catgcgctcc ggcgtgatca gctcgagcga
     4501 ctcgaccggc gcccggagcg gcagccgcac cgccggcggt ggcgtttcgt cctcctcccc
     4561 ggcgcggaac gccgccagtc cgtcgcgcgt cttctcatcg aagaacggca ggaccacgag
     4621 gtcgaccttc gagtcctgcc ccgcggccgg ggcgctttcg ccgggagccg cctgcccgga
     4681 gccgttctcc tcgcccgtct gcgccccgtg ggcgctcccg gcgaccgccg cggccgggtt
     4741 gaaggggagc gcccccttcg cggccggccg cagccccggc gggaattgcg accggaggaa
     4801 cgtcagcacc tcgccgaggg ccggcggagt cggcttcgtg gcggccgtgg ccggctcgga
     4861 cgctgtcccg ggctctggtg tctcggacgt gaccggctcg gccatcgtct caggctccgg
     4921 tgtctcggac gcgaccggct cgggggccgg ctgcaggggc gcgggcgcgg ctggcgtgga
     4981 ttcaggaggg gcctgcgcct cgcggaagat gaccacttcc gcgccggcgg acatcgcttc
     5041 gagatagccg gcgatggcgg ccgggatcgc cccttcctcc ggcagcgccg aatcggtcac
     5101 ccagaccggg gcgcgcggat gccccttgtc gcgcacggcc atgacccgct cgagcgccgt
     5161 cacgtgggag gcggagacga cgtcgaggta cggcgcggcg tcctcggcga agagctgctc
     5221 cggccaggcc tcgtccagga ggtcgaacgg cgggccgagg atgaccgcgt tcgagccggc
     5281 ggcgcggatc gacacgcggg cgttcttcag aataaaagcg tagtccttcg ccggcatcct
     5341 gccggcctcc tcgccgagga gctggtaggc atcgacccga tcgccgaggg cggtcgccaa
     5401 tccgagcaga cgatcggtcc agccggtccc cgcctcgcgg tcggcctcct ccaggcgcac
     5461 cagggtcttc aggctccggc cgcgcgcctc gtccaggagc gccaccagcg cggccggatc
     5521 gtcgaggtcg gcgcgccgcg ccgcgaacga cgcccagcag ggcgtctccg gcgtcgcgct
     5581 tcgaagtcct tcgggcaggg cggcagcggc cgcgaggcct gtcccggcgc gggattgggg
     5641 agggcctttt ccggaagtct gggatcgcgc cgaggcgggt atgaacaacc cgagcgcgat
     5701 cagcgggatg agtgcgaagg ttcgcagcat cttggcccgt gggggccgcc tagaaatcta
     5761 tcatccgcgc gggccggccg ccactcgccg ccactccaag acgaggcggt tcaacaggtt
     5821 gacttgcctt attcgggcac ttatcgttcc ggggtccaga ccatccgcgt ggggaaagag
     5881 gagaagctcg atgcagtatt ccgtcagtcc cgatggtgaa aagttcccgc tgccgacgcc
     5941 cgaggagtat cagaaggaat tcgcccggct cgagtccctg accgcggcgg agcgggcgaa
     6001 ggggcgtgaa atcgtcgtcg tcgtgggggt cggcttcgtc ggcgcggtga tggcggcggt
     6061 ggtcgccgac agcagcgaca agaagaccgg ccaggcgggg aagttcgtca tcggcatgca
     6121 gcggccgagc acccgttcct actggaagat cccgctcctc aatcgcgggt tgtcgccggt
     6181 gaagtccgag gacccggagg tggacgtcct catccagcgc tgcgtcaagg agcgcaagaa
     6241 cctcgtcgcc acctataccg aagacgccct gaagctcgcc gactgcgtcg tcgtggacgt
     6301 gcagtgcgac tacctcaagg agagcctcgg cgacctcaag accgggcacg ccgacatgga
     6361 ggcgctggag aagtcgttcg tctccatcgg ccggctcatc cagccgaact gcctcgtcct
     6421 catcgagacg acggtcgccc cggggacgac cgagttcatc gcctacccga tcctcaagaa
     6481 ggagttcgag aagcgcggca tcacgacggc gccgcttctg gcccactcgt acgagcgggt
     6541 catgccgggg cgcgactacg tccgctccat ccgggatttc tggcgcgtct gcgccggcgt
     6601 caacccggag agcaggaagc gcgtcgtggc gttcctctcc gaggtgctca atgtcgacac
     6661 gttcccgctg acggtgctcg accggccact cgagagcgag acctgcaaga tcgtcgagaa
     6721 ctcctaccgc gccacgatcc tcgccttcct cgacgagtgg agccgcttcg cggagagaaa
     6781 cggcgtcgac ctcgtcaagg tgatccaggc gatcaaggtc cggccgaccc acgacaacat
     6841 catcttcccc ggccccggta tcggcgggta ctgcctgccg aaggacggcg gcctggggat
     6901 ctgggcgaac cggcacctga tgggttggga ggaggagacg ttcaagatca cccccctcgc
     6961 catcaacatc aacgacacgc gcgcgctgca cgtcggcacg ctcacccgcg acgcgctgcg
     7021 gaacatgggg aaggtggtgg ccggatcgca cctcgtcctc ctcggcgccg cctaccgcga
     7081 ggacgtcggc gacacgcgct acagcggctc ggagctgatc gtccggaagc tcgcggagat
     7141 gggcggcgac atggcggtgc acgaccccta cgtcatgcac tggtgggagt tcgaggagca
     7201 ggagacctac ccgcgcgagg ggtactcctg gtcccgcttc ttccgcaacc aggaggggct
     7261 caagaaccta cggatgcagc aggacctcgg cgcggccctc aagggggcgg acgccgtcat
     7321 cctggcggtg cgccacaagc cttatctcga gctccaaccg gaggacgtgg tgaagcaggc
     7381 cgggcggccg ctcgctgtcg tggactgctt cggcatcctc gacgacgcga agatccgccg
     7441 ctatttcgag ctcgggtgcg aggtgaaggg gctcggccgc ggccacatcc agcgcatcaa
     7501 agaggacgtc cgcagcaaac gttgaaggtc cgctcgaccg cccgcggccc tagcgggaga
     7561 cggccgccgc ggtttgcggc gtggccgcgc gaaagagcgt ctcggcggcg gcgaccgagc
     7621 ccgcgccgac ttcgccgcgc atggcccctg cgatgagcgg gcgcagctcg tcgagcgcca
     7681 cgtcggtgtc gagcacccgc ggcgtcgtct gccccggcag cttgatgagc agcggcacgt
     7741 gccgcttcgc gcggtgctca tctccgggcg cgatcgccgg atcctccctc caggaatggt
     7801 ccgcagtcat gatgatcagg gcgcggtcgt agtctccggc gcgcttgagg cgctcggtca
     7861 cctccccgac caggcgatcg acgtacagca tctggcggtg atagtcctcc ggcgtgccgg
     7921 ccatgatgtc ggagtccacg tccgcggtcg ccccgctgtg gatcgggaac ggcccgcggt
     7981 agctgccgtc gggattgaag acgaacggcg cgtgcggcgc ggggaagtgc acgaacacca
     8041 gcgcgcgccg cggcgccgtc ccgacgatgg cgaagaggtc gtcccgcacc cggtgattca
     8101 gccgcaccca gtgccgcgag aagacgcgcg cccagatccc cttccacagg ccgctgatcc
     8161 ccggcatgaa ccagtactgc ggcgctccga gcgtggtgac gaccatgcgc tcgacgagcg
     8221 tgtccccctt cgggtagttc gaataggccc ggtccacgtc ggcttcgttc cccatgatgt
     8281 gcgggtacgg caggtagaag ccgacgagat acgtcgtgta gcggttccgg cgggccaggc
     8341 ccaggagcga ttccggcgcc ccggacggcg ccaggttgtc ggtgatgtgc tcgggccggg
     8401 gtccgctcgg ctcctcgggg tccttgcgat cgatgaagac gtagggcagc gcctcgtcgt
     8461 cgcggctctg gaagaggatg cgcggaagcg acacgtgcgt gctcgacgcc ggagcgcgcg
     8521 ccgtccggaa gaacagagcg tgctcctgga aggcccgcac attcgcgaac tcgggccgga
     8581 cctcgtggcc ctcgaccatg cgctggtacg accactcgtc gaagaccagg aaatagaccg
     8641 gcgcgccgtt ctccccttcc ccggacgcgg ccggaatcgg gtcgcgcgtg tagtcccagg
     8701 aacgccagaa aagggccggc cccagcagca ggagcaccag cggcgaaaag acgaaggtcg
     8761 ccccgcgcgc aatgcggatc atccgctcgc ggcgcagcca gaccccgagc acccaggcga
     8821 tcgccaggat cccggaagcg atgtcgagca ggacgccggc ggcgccgcga tgcgcgcggg
     8881 cgaactccag gagcgcctcg atgtgcagca gcggcagcag gttggcgagg attccgagca
     8941 ggaggaaggt caggaacacc acgtccttga gcccgcggag gatccggatc gcggtgcgat
     9001 cgatcgccaa ccctgcgagc gccgcggtcc cggagagcag caggatcgac aggaggatcg
     9061 ccagcgtgtc cttgcgcccc cagaagagga aatagcggtt gcgctcggtt ccgagcgccg
     9121 cgaggaacgg cgtgtagaag agaaagaccg cgacgagcga cggaatccag cggtcgagga
     9181 tcgccgccag ggtcgcgacg cgggaccgcg ctggtccgga cgtcatcgac cggtcacgcc
     9241 gcgaccgcgc ggcgcttgcg gaacagcagc aggacgcggc ctccttcagg gaccggttcg
     9301 cgcgccacga ggtcgaagcg gggggcgaac gcggcgaggc agcgctcgag cgtcatcccc
     9361 tcgaacgtct cgcggcgcag gcgcgtcagg cgacggaaca tgacatcgtc ggtcggcacg
     9421 aattcgagga cgagcgcgtc gcgcgtcagg tcgtcgaaga gcgaggcgat cgcctcgagc
     9481 ggcaggttcc ccgagacgtg caggtggtgc agcaacgcca gcgccagcac ggcgtcggcg
     9541 ccgatccgct ccaccaggcg cggccgctcg aggttgcgga agccgatggc ggggctcggg
     9601 ttgcagaggt cgacgacgat cggtgtgatc ggcttgtcct ggccgcggac gcggcggtac
     9661 agcatctcga cggcgtcgtg gtcggcgtcg gcggcgatga cctcggagcc ggcctccgcc
     9721 gcgagcagcg agtagacgcc ggtgttgcag cccaggtcga gcacccgccg cggcttcacg
     9781 cgatcgagca tggcggcgac gatccgcttc ttggcggcgg tggccgcgtc cacgtaggag
     9841 cactcctgcg tgtagcgcgc ccagaccccc tgcggccggt agccggcggc gagcgccagg
     9901 accttcttgc gcaggcgcct caggttcatc gcctgcggcg cgctgttcgg gttcggcctg
     9961 tcgaacacct cgcgccccgg cccgtcttcg ccgcttctcc ggtcgagcca gagcggcagg
    10021 gtcagatcga ggagcagccc cgggcgcagg cgcgacagcc cccggaacga ctcgcccacc
    10081 tgcgccgccg tccgcccgcc gaggctcggc aggaagtacg agcgcaggtc ccagccgcgg
    10141 tatctcgcca gcagcagcgg gtacgtgaac atctgctgga actgcccgag cgccacccag
    10201 atgtcgaggc gcggcggccg ctcgatcgag gcgaggtcga tgaacagggg gcgggcgcgc
    10261 gcgaactgca cgttgtaggc ggtcgcgtcc ttgagagagt ggccggtctc gaggagccgg
    10321 agctgcacgt cgagcgtcag ggcggcggcg tcggcgagca tcgacacggt ccagcagtag
    10381 ggataggtga tcggatcgat cgcgtcgtgc tcgaggaagc gggccgcgcc cgcgtgctcg
    10441 atcgccagcc ggtcgaggag cggggacgac gcgacgaacg aggtcgggac gacgagccgg
    10501 tcggcgacga gcctctggag cgatcccatc tccaagaggt ccaggagatc ggcggcggag
    10561 cgcgcgtcga gcgcccggaa gaccctcccc gcgtgccgga agacgtatcc cgccggatcg
    10621 cggaacgacg ccgcgtcgcg ctggctgccc gtggcggcct cggacatcgg cctcaggaaa
    10681 ccttgggttc cttctctgcc ggcgttgcgg tcgggggagt cgggcccggg gaggagaggg
    10741 gagcggccgg acgcttgatg agggcccaga gcgacttgcg gaagaacgcc gccccgcccg
    10801 cgaccgcggc caccagcgtc tggatcagga tcgacccgga gaccgggtcg atataggcca
    10861 gacggacgtg ccattccacg gatccccctc caccctgaca ggcagcgtca aatgatgggt
    10921 taagttgtta cgaagtcaag cgatacgatt tcgtgagggg gcatcaggga aggatgctac
    10981 gcagcccctc cgggtccgtg gccccctcgg tattgagggc caggacgcac gtggaggggc
    11041 cgagaccgag cctttccgtc gctgcgagga gggcatcggc ctgtttcagg gcgatgaggc
    11101 aggccacccc ggcccccccc gactcgcccg cctcgatcgg cggctccccg gccgtccccc
    11161 cgccccgcca gagcagccgg accgcctctt cggcgtagcg gtcctcgatg ctgaggaaca
    11221 ggtcgatccc ccgccggagt gtcggccagg cggcgaggga gggcagcccg cagttgagcc
    11281 cggccatgat cgagtcctgg cggccccgtc cggggcgggg ctgcccgtcg ggggagagga
    11341 tcgactccag caggcagtcg gcctcgaccg gctccaccac gaccaggcgg ggaggggtgc
    11401 ggcgctgccg gtagtgatcg accgcggcgg cggcgagcgc cccgaccccg ccctggagca
    11461 tcacgaggtc cggctgcggc aggagccgct cctcgatctg ggcgtccgtt tcctggaaca
    11521 gagtgctgta cccctcggcc accttgcgcg ggatgtccag gtacccctcg tagccggtgt
    11581 cggcgatcac ctgccagctg tttctcgccg cctcctccgc ggcgcgccgc accgtgtcgt
    11641 cgtaggtccc ttcgaccgtg acgacgcgcg ccccctcccc ccggatcgcc tcgatgcggg
    11701 ccctcgcggc gtgcgccggg atgaagacga ctgcctggag gccgagccgg cgggccgccc
    11761 aggcgaccgc ccggccgtgg ttgccgtcgg tcgcggtcgc gacggtttcg ggtggcgcct
    11821 cgcccgcacc ccggcgctct tcgaccaggg cgtgcagcgc ccacgacgcc ccgagtgcct
    11881 tgaaggcctt gatcccgaag cggcgcgact cgtccttgac gaggagcgtc ccgatgccga
    11941 ggagacgcgc ggcggtcggg agggagtgca ggggggtcgg ctcgtagccg gggagagagc
    12001 gatggaacgc gaggacgtcg cgctgagcac cggagggggt gaccactccg gcacggaaga
    12061 acggattgaa cgcgaagcgc atcaagaccg gccgcgaccc gcccgcagtc tatccgaggc
    12121 gggccgcggc cggcgggggg ggcgctacgg acgggccgag gcgagctcga gcgcctggcc
    12181 gcgcggcgcg cgcaggctgt aggcgaagac gacatccttg ctga
//
LOCUS       DASRIQ010000522         8253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3604774, whole genome shotgun sequence.
ACCESSION   DASRIQ010000522 DASRIQ010000000
VERSION     DASRIQ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3604774"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFD06_12800"
     CDS             complement(<1..727)
                     /locus_tag="VFD06_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HZN04453.1"
                     /translation="MNRLLAIAELGQSIWLDYIRRDLILEGGLARLVKEDGLKGVTSN
                     PAIFEKAIAQSPHYHRNIQALLRQGDDDAKGLFEALAIRDIQDATDVLLPAYEATRAR
                     DGYVSLEVSPYLARDTEATIAEARRLWAKTVRPNLFIKIPATPEGVPAIRQAISEGIN
                     VNVTLLFAQEAYEAVAKAYIEGLEAYVARGGDPSKVASVASFFISRIDTLADALLQKK
                     IAAASGPDKARLERLLGKVAIANG"
     gene            complement(754..2331)
                     /locus_tag="VFD06_12805"
     CDS             complement(754..2331)
                     /locus_tag="VFD06_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HZN04454.1"
                     /translation="MSETGVRKTVGGSGGAVHDGGAGALIQPLDEHNRRLLANVHPQP
                     YANPRPKERYHLVVLGAGTGGLVTAAIGAALGAKVALVERHLAGGDCLNVGCVPSKAL
                     LRAARAWSEARRAAREFGGPAAAGPGDFGVVMERMRRLRADLSRNDSVARFTGLGVDV
                     FIGEGRFTGPDALVVNGATTLRFRRAVVATGGRPAVPSVPGLREIEPLTNETVFGLTT
                     LPARLVVIGAGPIGCELAQAFARFGSRVTVVNRGERILPKEDADAARIVEEALRRDGV
                     RFLLGSRLIGARRDGGVRTLLFEHQGKHIEEQAEEILVAAGRAPNVESAGLGAAGIAF
                     DDKGVRVNARLRTTNRRVYAVGDVSSRFQFTHAADAQARLVAQNALFFGRGRADRLVM
                     PWCTYTSPELAHVGLSEAEARARGLAVDTLTVSLEEVDRAVLDGATEGFARVHLEQGG
                     DRILGATIVAEHAGDLIGEITLAMTAKTGLGAIGSTIHPYPTQGEVLRKLADAWRRRK
                     LTPQARRAFGVFFRVFK"
     gene            complement(2328..3074)
                     /locus_tag="VFD06_12810"
     CDS             complement(2328..3074)
                     /locus_tag="VFD06_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HZN04455.1"
                     /translation="MSGEGGSATGSAGAPPARGLPTLLKVIAAVVALALLALGGRALA
                     SSLTVFASWVQGLGAWGPIAFVAGYVAACVAFVPGAILTLAAGAIFGLGRGVLYVFIG
                     AVLGSSAAFLVARHLARRAIEKRIAGNPRFAAIDQAIAREGLKIVLLLRLSPVFPFNL
                     LNYGLGLTRVSFRDYLIGSIGMLPGTVLYVYYGTALGSLAQIASGARPQGGRAGTALL
                     VAGLLATIAVTIVITRAARRALARATGGQA"
     gene            complement(3071..5110)
                     /locus_tag="VFD06_12815"
     CDS             complement(3071..5110)
                     /locus_tag="VFD06_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HZN04456.1"
                     /translation="MDRGPVTEWLETDGLGGFASGTAGLIRTRRYHALLLTAAEPPGR
                     RFVLVNGLEALVVTPAGSVAISAQVYAGSAESAAPVVHPIGMEFIRRFDIEPWPAWRF
                     VLPDGIGIEQSIAAVQGRPIVTIAWRLLAARPGVSLVVRPLLSGRDLHALHRENPVFR
                     FEEERLGQAIVWRPYPGVPAISACANGDYTREPLWYRNFLYTEERERGFDHLEDLASP
                     GRFTFNLSRGTAALILSADTPGLAGDGGELPALAASGPLPSLRGLRARERRRRLRFSS
                     RLHRSADAYLVRRGGGRTVIAGYPWFSDWGRDTFIALRGLCLATGRLDDARSILLLWA
                     GFVRDGLLPNRFPEEAGGAPGAGPAGGTARDDASVDAPLWFIVTAHDWIEAMSAARRR
                     VARDDEAALVRSIEATLDAFLAGTRHGIRANRDGLLAAGDPGLALTWMDARVAGQPVT
                     PRAGKPVEIQALWINALRIGSARSDRFAAPLARASESFGRRFWHDAGAFLRDVVDVDH
                     EPGRDDATFRANQILAVGGLPFPVIDGDRARAVVDAVEHRLLTPIGLRTLDPADAAYR
                     PRYEGGPEERDRAYHQGTVWPWLLGPFVEAWVRVRGDGIEIRREARRRFLEPLAHHLD
                     MAGLEHLPEIADGATPHHPRGCPFQAWSVAEALRLSHVVLAEPRRTASGPRAGAR"
     gene            5298..8192
                     /locus_tag="VFD06_12820"
     CDS             5298..8192
                     /locus_tag="VFD06_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HZN04457.1"
                     /translation="MKPLRAAFAIVLVLAAGHAGIAQAQEGDCLEPGLLGDPVSLAGV
                     VAFDGIRGPAVARGDGEFAVVARQPEYSWDRLLFRVSDAGDILGEPTVLGTGDPGLPD
                     YSSVVWNGSGYAVAWYDQGLDRIAGVRLSATGQVIGSLINLPALHGDPFSGPGLAWNG
                     SEYGLAYIDDASSGESRLIFARLSAAGDLVGAPRVLAQGQVGTARLAAGASGYGVAWT
                     GQDVLSQHRVHFLALSPDGTPIGSETLSLDPQSAAGYPEVTAAAHRYVVVWAFDQGIR
                     LAFMTSGSAVDGVILHLTESPGPPAASVAWTGSSLMVVWGTFTDTNDLHARRVAENGT
                     FPDHEVRLTWSGQTPIPGGLVWSGDRFGLGWSSGFDSGHEGRFGFLGCACRDADSDLH
                     TLCSGDCDDNDPTVHPYAEEVCDGVDNGCNGGIDEGLDVPIQCGVGDCLRTVTPCVAG
                     VPATCVPGLPLPELCNGVDDNCDGLVDNSVDSDGDGVFDCVDCAPSNPLIYPGGPERC
                     NGSDDNCNQEVDEGLAIPFQCGVGNCLRTVTFCVAGVPATCAPGVPLPEECNGLDDDC
                     DGAIDNSDADQDEYGICADCAPLNPSIHPGAVEVCNGVDEDCDQVVDEGLAVPISCGR
                     GECERTVIRCQNGTPNVCIPGTPLYDVCNGLDDNCDGLVDNGDLDADGSSDCIDCALL
                     NPAIHPGAVETCNGIDDNCDQIVDDFPAFVDVDGDGVRLCDNCPSEPNPGQENQDQDQ
                     FGDACDTCPLVPNILVDSDHDGFPDACDLCPFVPYVTTDTDEDGLGAACDNCPNLFNP
                     EQENADRDAYGDVCDRCPHGNIFNDDTDADLLADECDNCPFNPNPDQTDVNVDGQGDA
                     CDLDDGLLMIWVTRPDEVNWDPETPFLSYDVYRGDLDVLKATGESTQDPAVVPLAARS
                     CGQLEPFLVDDAPPVGKAVYYLVAVTTASGYEGIGNDSAGNPRLNPHSCP"
BASE COUNT         1251 a         2865 c         2922 g         1215 t
ORIGIN      
        1 tgccgttggc gatcgccacc ttcccgagca gccgctccag gcgcgccttg tccgggccgg
       61 aggcggcggc gatcttcttc tggaggagcg cgtcggcgag cgtgtcgatg cggctgatga
      121 agaaggacgc gacgctcgcc accttcgacg ggtcgccgcc gcgtgccacg tacgcctcga
      181 gtccttcgat gtacgccttc gcgaccgcct cgtacgcctc ctgggcgaac agcagcgtga
      241 cgttgacgtt gatcccctcg ctgatcgcct gccggatcgc cggcaccccc tcgggcgtcg
      301 ccgggatctt gatgaacagg ttcggccgga cggtcttcgc ccacaggcgg cgggcctcgg
      361 cgatggtcgc ctcggtgtcg cgcgccagat acggggacac ctcgaggctg acgtagccgt
      421 cccgcgccct ggtggcctcg taggccggca gcaggacgtc ggtcgcatcc tggatgtcgc
      481 ggatggcgag ggcctcgaaa agccctttgg cgtcgtcgtc cccctggcgc aggagcgcct
      541 ggatgttgcg gtgatagtgg gggctctggg cgatcgcctt ctcgaagatc gccgggttgg
      601 aggtgacccc cttgaggccg tcctccttca cgagccgggc cagaccccct tcgaggatca
      661 ggtcgcggcg gatgtagtcg agccagatgg actggcccag ttcggcgatg gcgagcaggc
      721 gattcatgcg tcgtcctctg ggagcagacg attctacttg aagacgcgga agaagactcc
      781 gaaggcccgc ctggcttggg gcgtcagttt gcgccgtcgc cacgcatccg cgagcttccg
      841 gaggacctcc ccctgtgtcg ggtaggggtg gatggtggag ccgatcgcgc cgaggccggt
      901 cttcgcggtc atcgccaggg tgatctcgcc gatgaggtcg ccggcgtgct ccgccacgat
      961 cgtcgccccc aggatgcgat cgccgccctg ctccagatgg acgcgcgcga acccctcggt
     1021 cgcgccgtcg agaacggcgc ggtccacctc ttcgagagac acggtcaagg tgtccacggc
     1081 gagtccgcgg gcgcgggcct ccgcttcgga gaggccgacg tgcgccagct ccggagaggt
     1141 gtacgtgcac cacggcatca ccagccggtc ggcgcggccg cgcccgaaga agagcgcgtt
     1201 ctgcgccacg aggcgggcct gcgcgtcggc ggcgtgcgtg aactggaagc gggacgacac
     1261 gtctccgacg gcgtagacgc ggcggttggt ggtgcggagc cgcgcgttca ccctcacgcc
     1321 tttgtcatcg aacgcgatgc cggcggcgcc gagtccggcc gactcgacat tcggcgcccg
     1381 gccggcggcg acgaggatct cctcggcctg ctcctcgatg tgtttcccct ggtgctcgaa
     1441 gagcagcgtc cggacgccgc cgtcgcgccg cgcgccgatg aggcggctgc cgagaaggaa
     1501 acggaccccg tcgcgccgca gcgcctcctc gacgatccgg gcggcgtcgg cgtcctcttt
     1561 ggggaggatg cgctcgccgc ggttcacgac cgtgacccgg ctgccgaagc gggcgaacgc
     1621 ctgggccagc tcgcagccga tcggcccggc gccgatcaca acgagacgcg cgggaagggt
     1681 cgtgagcccg aagaccgttt cgttggtgag cggctcgatc tcccggagcc ccgggaccga
     1741 cgggacggcc ggccggccgc cggtggcgac gacggcgcgg cggaagcgga gggtcgtcgc
     1801 tccattcacg acgagagcgt cggggccggt gaagcgcccc tcgccgatga aaacgtccac
     1861 accgaggccg gtgaagcggg cgacgctgtc gttgcggctg aggtcggccc ggaggcgccg
     1921 catcctctcc atgacgacgc cgaaatcccc cggtccggcg gcggcggggc cgccgaactc
     1981 gcgggcggcg cgccgcgcct cgctccaggc gcgcgccgcg cgcagaagcg ccttcgacgg
     2041 cacgcagccg acgttgaggc agtccccgcc agccaggtgt ctctcgacca gcgccacctt
     2101 cgctccgagc gccgcgccga tggcggcggt gacgagtccg ccggtgccgg cgccgagcac
     2161 cacgagatga tagcgctcct tcggccgcgg attcgcgtac ggctgcggat ggacgttcgc
     2221 gagaagccgc cggttgtgct cgtcgagagg ctggatcagc gcgccggcac cgccgtcatg
     2281 gactgcgccg ccggaaccgc cgaccgtctt tcgcactccg gtctcgctca tgcctgccct
     2341 cccgtggcgc gcgcgagcgc ccggcgggcg gcgcgcgtga tgacgatcgt gaccgcgatc
     2401 gtcgcgagaa gccccgcgac gaggagcgcc gtccctgcgc gtcccccctg cgggcgcgct
     2461 cccgacgcga tctgcgccag gctcccgaga gccgtgccgt agtagacgta gagcaccgtc
     2521 cccgggagca tcccgatgga gccgatgagg tagtcgcgga acgacacgcg cgtcaggccg
     2581 agcccgtagt tgagcaggtt gaacgggaag accggggaca gccgcagcag gaggacgatc
     2641 ttgagccctt cacgggcgat cgcctgatcg atcgccgcga acctcggatt tcccgcaatc
     2701 cgcttctcga tggcgcggcg cgccaggtgg cgggccacga gaaacgccgc cgacgatccg
     2761 agcaccgccc cgatgaagac gtagaggacg cctcggccca ggccgaagat cgcccccgcc
     2821 gcgagggtca ggatcgcccc cggcacgaag gcgacgcagg cggcgacgta cccggcgacg
     2881 aaggcgatcg gtccccacgc cccgagcccc tgcacccagg aggcgaagac ggtgagcgaa
     2941 gaggcgaggg cgcgcccgcc gagggcgagg agcgcgagcg cgacgaccgc cgcgatgacc
     3001 ttgaggagcg tgggcagccc gcgggcgggt ggggcgcctg ccgacccggt cgcgctgccg
     3061 ccttcgccac tcatcgggca cccgcgcgcg ggccgctcgc cgtgcggcgc ggctccgcca
     3121 ggacgacgtg cgagaggcgc agcgcctccg ccaccgacca ggcctggaac gggcagcccc
     3181 gggggtggtg cggggtcgcg ccgtcggcga tctccgggag gtgctccagc cccgccatat
     3241 caagatgatg cgccagcggc tcgagaaagc gtcgccgggc ctcccggcgg atttcgattc
     3301 cgtcaccgcg cacccgcacc cacgcttcga cgaacggtcc gaggagccac ggccagacgg
     3361 ttccctgatg gtaggcccgg tcgcgttcct ccggcccccc ttcgtagcgg ggacggtagg
     3421 cggcatcggc cggatcgaga gtgcggagtc cgatcggagt gagcagccgg tgctcgacgg
     3481 cgtccaccac cgcgcgggct cgatccccat cgatcaccgg aaacggcagg ccgccgacgg
     3541 cgagaatctg gttggcgcga aacgtggcgt cgtcgcgccc cggctcgtga tcgacgtcca
     3601 cgacgtcgcg gaggaaggcg ccggcgtcat gccagaagcg ccttccgaac gattcggagg
     3661 cgcgcgcgag cggagcggcg aatcggtcgg agcgggccga accgatccgc agggcgttga
     3721 tccagagcgc ctggatttcc accggcttgc cggcgcgcgg cgtcaccggc tggcccgcga
     3781 cgcgcgcgtc catccaggtc agcgccagtc cgggatcgcc ggcggcgagg aggccgtcgc
     3841 ggttggcgcg gatgccgtgg cgcgtcccgg caaggaaggc atcgagggtc gcctcgatgg
     3901 accggacgag cgccgcctcg tcatcgcggg ccacgcgccg tcgcgccgcg ctcatcgcct
     3961 cgatccagtc gtgcgccgtt acgatgaacc agagcggcgc gtccacgctg gcgtcgtcgc
     4021 gcgccgtgcc gccggccgga ccggctcccg gcgccccgcc cgcctcctcc gggaaacggt
     4081 tcggcaggag gccgtcccgg acgaagccgg cccacagcag caggatcgat cgcgcgtcgt
     4141 cgagccgtcc ggtggcgaga cacagaccgc gcagcgcgat gaaggtgtcg cgaccccagt
     4201 cggagaacca cgggtacccc gcgatgacgg tgcgcccgcc tccgcgccgc acgagatagg
     4261 cgtcggcgga tcggtgcagc cgcgacgaga agcgaagtcg ccgccgccgc tcgcgtgccc
     4321 gcagtccgcg gagcgaaggg agcggcccgg aggccgccag cgccggcagc tcgccgccat
     4381 ccccggcgag ccccggtgtg tcggccgaca ggatcaacgc ggcggtgccg cgcgacaggt
     4441 tgaaggtgaa gcggccgggg gaggcgaggt cctcgaggtg atcgaagccg cgctctcgct
     4501 cctccgtgta caggaagttc ctgtaccaga gcggctcacg cgtgtagtcg ccgttcgcgc
     4561 aggcgctgat cgccggcacg cccgggtacg gccgccagac gatcgcctgg ccgagccgct
     4621 cctcctcgaa acgaaagacg ggattctctc ggtgcagcgc gtgcaggtcg cgccccgaga
     4681 gaagcggccg gaccacgagc gacacgcccg gccgcgccgc gagcagccgc caggcgatgg
     4741 tcacgatcgg ccggccctgg accgcggcga tcgactgctc gatgccgatg ccgtccggca
     4801 ggacgaaccg ccacgccggc cacggctcga tgtcgaaccg ccggatgaac tccatcccga
     4861 tgggatgcac gaccggggcc gccgattcgg cgcttccagc gtaaacctgc gcggagatcg
     4921 caaccgaacc ggccggcgtg acgactaacg cttcgagacc gttgacgagt acgaagcggc
     4981 gcccgggagg ctcggccgcc gtcaggagga gggcgtgata gcggcgagtc cggatgagcc
     5041 cggcggtccc ggaggcgaat cccccgagtc cgtccgtctc gagccactcg gtcaccggtc
     5101 cccgatccat cccttctccg cccctcttcg gatcacgggt ccctcgcgcc gcgggagtct
     5161 aacctgagca ccttccaagt ctgaatatgt tgacgccgca tccgcttcgg ggtatttagg
     5221 ctctcaaatc gctctgtccg gggagggctt ccgatgcgtc aggcgcttct ccatccgttc
     5281 acggtccctt ccgccgcgtg aagcctcttc gcgcggcatt cgcgatcgtc ctggtgctcg
     5341 cggccggtca tgccggcatc gcccaggcgc aggaagggga ctgcctggag ccggggctcc
     5401 ttggggatcc ggtctccctc gccggtgtgg tggcgttcga cgggatccgg ggcccagccg
     5461 tcgcgcgcgg cgacggggag ttcgccgtcg tggcgcgcca gccggaatac tcgtgggatc
     5521 ggctgctctt ccgggtctcg gacgctgggg acatcctcgg agagccgacg gttcttggca
     5581 caggggatcc cgggctcccc gactactcca gcgtcgtgtg gaacggaagc gggtacgccg
     5641 tggcctggta cgatcagggg ctcgaccgga tcgcgggtgt gcggctgtcg gcaaccggcc
     5701 aggtgatcgg ttccctgatc aacctgcccg ccttgcatgg ggatcctttc agcgggccag
     5761 gcctggcatg gaacggatcc gaatacggat tggcctacat cgacgacgcg tcctcggggg
     5821 aatcccgcct gatcttcgcg cggctcagcg ccgcgggaga tctcgtcggc gctccacgcg
     5881 tcctggccca gggtcaggtc gggacggcgc gcctcgccgc cggcgcatcc gggtacggcg
     5941 tcgcctggac cgggcaggat gtcctgtcgc agcatcgggt ccatttcctg gcgctctccc
     6001 cggacgggac tccgattgga tcggagacgc tctcgctcga tcctcagagc gcggccggat
     6061 acccggaggt cacggcggcc gcccatcggt acgtggtcgt ctgggctttc gaccagggga
     6121 ttcgcctcgc cttcatgacg tcgggcagcg ccgtggatgg ggtcatcctc catctgacgg
     6181 aaagtccggg gcctcccgcg gcgagcgtcg cgtggaccgg ctcctcgctc atggtggtct
     6241 gggggacctt caccgacacc aacgatctgc acgcgaggcg cgttgccgag aacgggacct
     6301 tccccgacca cgaggtgcgg ctcacctgga gcggccaaac ccccattcct ggcgggttgg
     6361 tctggagcgg cgatcgcttc ggcctggggt ggtcgtccgg cttcgattcg ggacacgagg
     6421 ggcgcttcgg gttcctcggc tgcgcctgtc gcgacgccga cagcgacctg cacacgctgt
     6481 gcagcggcga ctgcgacgac aacgatccga ccgtccatcc gtatgcggag gaagtgtgcg
     6541 acggcgtcga caatggctgc aacggcggga tcgacgaggg gctcgacgtc cccatccagt
     6601 gcggcgtggg cgactgtctc aggaccgtga cgccctgcgt cgccggcgtg cccgccacct
     6661 gtgtccccgg tctcccgctc cccgagctct gcaacggcgt cgacgacaac tgcgatggcc
     6721 tggtggacaa ctccgtcgac agcgacgggg atggggtctt cgactgcgtt gattgcgccc
     6781 cgtcgaatcc cctcatctat ccgggagggc cggagcgctg caacggcagc gacgacaact
     6841 gcaaccagga ggtcgacgag gggctcgcca ttcccttcca gtgcggcgtg ggcaactgtc
     6901 tcaggaccgt gacgttctgc gtcgccggcg tccccgccac ctgtgccccc ggtgtcccgc
     6961 tccccgagga atgcaacggc ctcgacgatg attgcgacgg agccatcgac aacagcgacg
     7021 ccgaccagga cgagtacggg atctgcgccg attgcgctcc tctcaatcct tccatccatc
     7081 cgggggcggt ggaggtgtgt aacggggtgg acgaggactg cgaccaggtc gtggacgagg
     7141 ggctcgccgt gccgatctcc tgtggcaggg gggagtgcga gcgaacggtt atccgctgcc
     7201 agaacggaac gccgaacgtc tgcatcccgg ggactcctct gtacgacgtt tgcaacggtc
     7261 tggacgacaa ctgcgatggc ctcgtcgaca acggtgacct cgacgcggat gggagcagcg
     7321 attgcatcga ctgtgccctc ttgaacccgg cgatccatcc gggcgccgtg gaaacgtgca
     7381 acgggatcga cgacaactgc gatcagatcg tcgacgactt cccggcgttc gtcgatgtcg
     7441 acggggacgg tgtccgcctc tgcgacaact gtccctcgga gccgaaccca gggcaggaga
     7501 accaggacca ggaccagttc ggggacgcct gcgacacctg tccgttggtg cccaacatcc
     7561 tggtcgacag cgatcatgac ggattccccg atgcctgcga cctctgtccg ttcgttccgt
     7621 atgtgacgac cgacaccgac gaagacggac tcggcgccgc ctgcgacaac tgtccgaatc
     7681 tcttcaatcc cgagcaggaa aacgcggacc gggacgccta cggggatgtc tgcgaccgat
     7741 gcccccacgg gaacatcttc aacgacgaca ccgatgccga cctgctcgcc gacgagtgcg
     7801 acaactgccc cttcaatccc aatccggacc agaccgacgt caacgtggac ggtcagggtg
     7861 atgcctgcga tctcgacgac ggcctgctga tgatctgggt gacgcggccg gacgaagtga
     7921 actgggatcc ggagacgccg ttcctgtcgt acgacgtcta ccgcggcgac ctcgacgtgc
     7981 tcaaagcgac cggggagtcg acgcaggacc cggcggtcgt cccgctggcg gcgcgatcct
     8041 gcggccagct cgagcccttc ctcgtggacg acgcgccgcc ggtggggaag gcggtgtact
     8101 acctcgttgc ggtcacgacc gcgtccgggt acgaggggat cggcaacgac agcgccggga
     8161 acccgcgcct gaacccgcat tcgtgtccat gatgcgtcga aaggaagtga tgcggcagcg
     8221 gagagtcttg cgattcggga tacttcttct ctc
//
LOCUS       DASRIQ010000523         5359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_451287, whole genome shotgun sequence.
ACCESSION   DASRIQ010000523 DASRIQ010000000
VERSION     DASRIQ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451287"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..367)
                     /gene="nuoK"
                     /locus_tag="VFD06_12825"
     CDS             complement(68..367)
                     /gene="nuoK"
                     /locus_tag="VFD06_12825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZN04458.1"
                     /translation="MPPDHVLTVSLLLFGVGVLGVLTRRNAIIILMSIELMMNAANLN
                     FVAFSRQHAHLTGQVFGVFTIAVAAGEVAVGLAIVLAVYRRFSSIHVDDVRLMKE"
     gene            complement(384..932)
                     /locus_tag="VFD06_12830"
     CDS             complement(384..932)
                     /locus_tag="VFD06_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZN04459.1"
                     /translation="MTLGPEAILFYVLSAVAVGAGLQVILSRSPIHSAIGLVTSFLAI
                     AALYVLLHAEFVAAVQVLVYAGGIMVLFLFVIMLVNLRDRAPLRMRWRGALSAGIGAL
                     AIGAMLFAVGRGRLAAAPIGAPAADVAAGVGNLQAVGAALFTDALLPFEIASVLLLVA
                     MIGAIVLARDLGRDLGREPGRE"
     gene            complement(929..1390)
                     /locus_tag="VFD06_12835"
     CDS             complement(929..1390)
                     /locus_tag="VFD06_12835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HZN04460.1"
                     /translation="MTGWLRSILLAELWVGLWTTLKNQFRPHVTVEYPKETVDLAPRF
                     RGTPRLRYHPENGEELCIACHLCEVVCPDDCIHIVSEKKPEGKGKRLVSFEINLERCC
                     FCGLCVDPCPTKPLTAIYMSHDYELADYRREPFVAPLKTLLDGTTPHEYTE"
     gene            complement(1387..2613)
                     /locus_tag="VFD06_12840"
     CDS             complement(1387..2613)
                     /locus_tag="VFD06_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HZN04461.1"
                     /translation="METRALVEQVVIPCLQVVITVLLIPLVIAYLQLVERKVLGFMQA
                     RLAPRRVGWHGLLQPIADAVKLLIKEDTIPARADRFLFTLAPIVSVVPALLALAVIPI
                     GGAPFTLFGYEVRPYIADLDIGVLFLLAVGSLGIYGILLGGWASNSKYSLLGALRSGA
                     QMISYEIPLGFALVGVMMLAGTASLVGIVEAQKSAGWWYVAPQILGFFCYFVSAVAET
                     NRIPFDLPEAEAELVAGFHTEYSGMKFAFFFLAEYTNMIVVSALVTVAFFGGWLRPFP
                     NVRALDWLENGWVLLVPLTLGAFLGWIVDQRVGGGMRFLFVGALLGGFLGFGIGAADL
                     LGVASALVPQISGAFWFGLKVSFFLFAYIWFRGTYPRYRYDQLMDLGWKWLLPMSIVN
                     VLLTGLVLLLRGGGAS"
     gene            complement(2613..3710)
                     /locus_tag="VFD06_12845"
     CDS             complement(2613..3710)
                     /locus_tag="VFD06_12845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZN04462.1"
                     /translation="MLRTEEMEINMGPQHPSTHGVLRLILHLDGETIAGMRPVIGYLH
                     RGVEKLSEHRTYAMIEPLTDRLDYVAAVSENFGWCGAVERLMGVTPPPRAEYLRVVLA
                     ELQRIASHLLWLGTHALDLGAMTVFFYTFREREMILDLFEAFCGARLTTNSMRIGGFL
                     EDAPRDWLARVRHFLKIFPGRMKEYEDLLTKNRIFVGRTLGVGVLSAQDAIAYGVSGP
                     PLRASGVSYDVRKATPYSAYPEFEFDIPVGKNGDTYDRYLVRMEELRQSCRIIGQALD
                     RMPDGEVRAKVPKYIKPPQGEVYHAVESPRGEQGFYVISEGGEMPYRVRFRAPSFANL
                     QALETMCRGRLVADVVAVIGSIDIVLGDCDR"
     gene            complement(3727..4344)
                     /locus_tag="VFD06_12850"
     CDS             complement(3727..4344)
                     /locus_tag="VFD06_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZN04463.1"
                     /translation="MAEETGPPPPAAAPPLPLAAEALKEALGDRVLEVKPQTVPPIVR
                     LAPGALIEGLTLLRDRPELRFDFLSSVTAVHWPKRERPFDVVYHLYSTTRRLRLAVKI
                     DLAEGEEAPTACGLWPAANWHEREVFDLFGIRFAGHPDMTRILMPDDWVGHPLRKEYP
                     LEGHPRDHLQYREVGTAEHVYNYGKAPIRGFGWKKEVEKTGVGRG"
     gene            complement(4358..4846)
                     /locus_tag="VFD06_12855"
     CDS             complement(4358..4846)
                     /locus_tag="VFD06_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZN04464.1"
                     /translation="MGLIEDRFQPNILTTTVDSLFNWARKSALWPMSFGLACCAIEMM
                     ATVASRYDIDRFGAGVFRATPRQSDVMIVAGTVCEKMAPVIKRLYDQMPEPKWVISMG
                     ACATCGGPFPTYSVVQGVDRIVPVDVYIPGCPPRPEALLYGFLRLQDKIAKMTIARRA
                     SA"
     gene            complement(4837..5193)
                     /locus_tag="VFD06_12860"
     CDS             complement(4837..5193)
                     /locus_tag="VFD06_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZN04465.1"
                     /translation="MVEYTPVLFLLLLAAVFPAATLVIAAALRPSGSREAAMEDAYEC
                     GVPPSGEARGRMPIRFYLLAVLFVIFDVEAIFLFPWAVQFKALGGFGFAAMVVFLGIL
                     LLGYAYAWKKGALEWV"
BASE COUNT          943 a         1861 c         1839 g          716 t
ORIGIN      
        1 ggccccagca gcccgttcgc cacgaacccc gcgaacggga ggagcgggac gagccagagg
       61 aggccgatca ttccttcatc agccggacgt cgtcgacgtg gatgctgctg aaccttcggt
      121 agacggccag gacgatcgcc agcccgaccg ccacctcccc cgcggcgacg gcgatggtga
      181 agacgccgaa cacctgtccg gtgaggtgcg cgtgctggcg gctgaaggcg acgaagttca
      241 ggttggcggc gttcatcatc agctcgatgg acatcaggat gatgatggcg ttgcgccgcg
      301 tcagcacgcc gaggacgccg acgccgaaga gcagcaggct gacggtcagg acgtgatccg
      361 gcggcaccgg catcgcgctc acctcactcc cgtccaggct cgcgtccgag gtcgcgcccg
      421 agatcgcgcg ccaggacgat cgccccgatc atcgccacga gcagcaggac cgaggcgatc
      481 tcgaacggca gcagcgcgtc ggtgaagagc gccgcgccga ccgcctggag attcccgaca
      541 ccggccgcga cgtccgccgc cggggcgccg atcggcgccg ccgcgagccg gccgcgtccg
      601 acggcgaaga gcatcgcgcc gatcgccagc gccccgatcc cggccgacaa cgccccgcgc
      661 caccgcatcc ggagcggggc ccggtctctc agattgacga gcatgatgac gaacaggaac
      721 agcaccatga tgccgccggc gtacaccagc acctgcaccg ccgccacgaa ctcggcgtgc
      781 agcaggacgt acagggcggc gatggcgagg aacgacgtca cgagcccgat ggccgagtgg
      841 atcgggctgc ggctgaggat cacctggagc cccgccccca ccgcgacggc ggacaggacg
      901 tagaagagga tcgcctccgg ccccagggtc actccgtgta ctcgtgcggc gtggtgccgt
      961 cgagcagggt cttgagcggg gccacgaacg gctcgcggcg gtaatcggcc agctcgtagt
     1021 cgtgcgacat gtagatcgcc gtgaggggct tcgtcgggca cgggtccacg cacaggccgc
     1081 agaagcagca acgctccagg ttgatctcga aggagacgag gcgctttcct ttcccctccg
     1141 gcttcttctc cgagacgatg tggatgcagt cgtcggggca gacgacctcg cacaggtggc
     1201 aggcgatgca cagctcttcg ccgttctccg ggtggtaacg gaggcgcggg gtgccgcgga
     1261 agcgcggcgc gaggtcgacc gtctctttcg gatactcgac cgtcacgtgg gggcggaact
     1321 ggttcttgag cgtcgtccag agcccgaccc acagctcggc cagcaggatc gaccggagcc
     1381 agcccgtcac gacgcccctc cgccccgcag cagcagcacg agcccggtga gcagcacgtt
     1441 gacgatcgac atcggcagga gccacttcca gccgagatcc atcagctgat cgtagcggta
     1501 gcgcgggtag gtgccccgga accagatgta ggcgaacagg aagaacgaca ccttgagccc
     1561 gaaccagaac gcccccgaga tctgcggcac caaggccgag gcgacgccta agaggtccgc
     1621 ggcgccgatg ccgaacccga ggaaccctcc gaggagcgcg ccgacgaaga ggaaccgcat
     1681 cccgcctccg acccgctgat cgacgatcca gccgaggaac gcgccgagcg tcagcggcac
     1741 cagcagcacc cagccgttct ccagccagtc gagcgcccgg acgttcggga acggccgcag
     1801 ccagccgccg aagaaggcga cggtcaccag cgccgagacg acgatcatgt tcgtgtactc
     1861 ggcgaggaag aagaaggcga acttcatccc ggagtactcg gtgtggaaac cggcgacaag
     1921 ctccgcctcc gcctccggca ggtcgaacgg gatgcggttg gtctcggcca cggcgctgac
     1981 gaagtagcag aagaacccga ggatctgcgg cgccacatac caccagccgg ccgacttctg
     2041 agcctcgacg atgccgacca gcgacgccgt cccggcgagc atcatgacgc cgaccagcgc
     2101 gaagccgagc gggatctcgt aggagatcat ctgcgccccg gaccgcagcg ccccgaggag
     2161 cgagtacttg ctgttcgacg cccagccgcc gaggaggatg ccgtagatcc cgagcgatcc
     2221 gaccgccagc aggaacagca cgccgatgtc gaggtcggcg atgtacggcc ggacctcgta
     2281 cccgaagagc gtgaacggag cgccgccgat cgggatgacc gcgagcgcca ggagcgccgg
     2341 gacgacgctg acgatcggcg ccagcgtgaa caggaagcgg tcggcgcgcg ccgggatcgt
     2401 gtcctccttg atgaggagct tgacggcgtc ggcgatcggc tgcagcaggc cgtgccagcc
     2461 gacgcgccgc ggcgccaggc gcgcctgcat gaagccgagc actttccgtt cgacgagctg
     2521 gagatacgcg atgacgagcg ggatgagcag gaccgtaatc accacttgaa gacaagggat
     2581 gaccacctgc tccacgagcg cccgcgtctc catcagcgat cgcagtcccc gaggacgatg
     2641 tcgatcgatc cgatcaccgc caccacgtcc gccaccaggc gcccacggca catcgtctcg
     2701 agcgcctgga gattcgcgaa cgacggcgcc cggaagcgaa cccggtacgg catctccccc
     2761 ccctcggaga tgacgtagaa cccctgctcg cctcgcgggc tctcgaccgc gtggtacacc
     2821 tccccctgcg gcggcttgat gtacttcggc accttcgccc gcacctcgcc gtccggcatc
     2881 cggtcgagcg cctggccgat gatgcggcac gactggcgca gctcctccat gcggacgagg
     2941 taccggtcgt aggtgtcgcc gttctttccg accgggatgt cgaactcgaa ctcggggtag
     3001 gccgagtacg gggtcgcctt gcgcacgtcg tacgacaccc ccgaggcgcg cagcggcggc
     3061 ccgctcacgc cgtacgcaat cgcgtcctgc gcgctcaaca caccgacccc gagcgtgcgg
     3121 ccgacgaaga tgcggttctt ggtcaggagg tcttcgtact ccttcatccg ccccgggaag
     3181 atcttcagga agtggcgcac gcgcgccagc cagtcgcgcg gcgcgtcctc gaggaacccg
     3241 ccgatgcgca tcgagttggt ggtcaggcgg gcgccgcaga aggcctcgaa caggtcgagg
     3301 atcatctccc gctcgcggaa ggtgtagaag aagacggtca tggcgccgag gtcgagggcg
     3361 tgcgtgccga gccagagcag gtgcgaggcg atgcgctgca gctccgcgag gacgacgcgc
     3421 aggtactcgg cgcgcggcgg cggcgtcacc cccatgaggc gctcgaccgc gccgcaccag
     3481 ccgaagttct ccgacaccgc cgcgacgtag tcgagccgat cggtgagcgg ttcgatcatc
     3541 gcgtaggtgc ggtgctcgga gagtttttcg acgccgcggt gcaggtagcc gatcaccggc
     3601 cgcatcccgg cgatcgtctc gccgtcgagg tggaggatga ggcgcagcac cccatgggtg
     3661 gaggggtgct gcggccccat gttgatctcc atctcctcgg tgcgcagcat caggacccca
     3721 ggcctcctac ccccgcccca cgccggtctt ctcgacttcc ttcttccagc cgaagccgcg
     3781 gatcggcgcc ttcccgtagt tgtagacgtg ctccgccgtc cccacttcgc ggtattgcag
     3841 gtggtcgcgc gggtgcccct cgagcggata ttccttgcgc agcgggtgcc cgacccagtc
     3901 gtccggcatc aggatccggg tcatgtccgg gtgccccgcg aagcggatgc cgaagagatc
     3961 gaacacctcg cgctcgtgcc agttggcggc cggccacaga ccgcaggcgg tgggcgcctc
     4021 ctctccttcc gcgaggtcga tcttgaccgc gagccgcagc cgccgcgtcg tcgagtagag
     4081 gtggtagacg acgtcgaagg ggcgctcccg cttcggccag tggaccgcgg tgacgctgct
     4141 caggaaatcg aagcgcagct cgggccggtc gcgcaggagc gtgagccctt cgatcagggc
     4201 gcccggggcc agccggacga tcggcgggac ggtctgaggc ttcacctcga ggacgcgatc
     4261 cccgagggcc tccttcagcg cctccgccgc caaggggagc ggcggcgcgg cggcgggagg
     4321 cggcggcccg gtctcctcgg ccatcgcctc aatcccctca ggcgctcgcc cggcgggcga
     4381 tcgtcatctt ggcgatcttg tcctgcaggc ggaggaaccc gtacaggagc gcctcgggac
     4441 gcggcggaca gccggggatg tagacgtcca ccgggacgat gcgatcgacc ccctgcacca
     4501 cgctgtaggt cgggaacggc ccgccgcagg tggcgcacgc ccccatcgag atgacccact
     4561 tcggctccgg catctggtcg tagaggcgct tgatcaccgg ggccatcttc tcgcacaccg
     4621 tgccggcgac gatcatcacg tccgactgac gcggcgtggc gcggaacacc ccggccccga
     4681 agcggtcgat gtcgtagcgc gacgccaccg tcgccatcat ctcgatggcg cagcaggcga
     4741 gcccgaacga catcggccag agggccgact tgcgcgccca gttgaagagg gaatcgacgg
     4801 tggtcgtcag gatgttcggc tggaagcgat cttcgatcag acccactcga gcgccccttt
     4861 cttccaggcg taggcgtagc cgaggagcag gatccccagg aagacgacca tcgccgcgaa
     4921 gccgaacccg ccgagcgcct tgaactggac cgcccagggg aacaggaaga tcgcctcgac
     4981 gtcgaagatc acgaacagca ccgccagcag gtagaagcgg atcggcatcc ggccgcgcgc
     5041 ctcccccgat ggcggcacgc cgcactcgta ggcgtcctcc atcgccgcct cccgcgatcc
     5101 ggacgggcgc agcgccgcgg cgatgaccag ggtggccgcg gggaacaccg cggcgagcag
     5161 gaggaggaac aggaccggcg tgtactcgac cacgtcggga gaatctatca cacggtgggg
     5221 gcccgatcac ggggccccgg cccgcgccgg ggacgcccgg acaattccta gggggcggcg
     5281 tggtgcagga cgcgccgggc gtagtcggtg gtgacggggg cgcgctcggc gacccgctcc
     5341 ggcccctcgt tgtaggccg
//
LOCUS       DASRIQ010000524         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1262011, whole genome shotgun sequence.
ACCESSION   DASRIQ010000524 DASRIQ010000000
VERSION     DASRIQ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262011"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFD06_12865"
     CDS             <1..342
                     /locus_tag="VFD06_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04466.1"
                     /translation="HLVDVSETTGRDPLHDIQVINGELRAFSEALAAKPQILVATKTD
                     TRPSPERLKMVEEEARRLGVPFRAISAASGEGVKGLLEAAWPLVQEAAQRETESGHRE
                     TEAPGEERPFS"
     gene            360..734
                     /gene="rsfS"
                     /locus_tag="VFD06_12870"
     CDS             360..734
                     /gene="rsfS"
                     /locus_tag="VFD06_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZN04467.1"
                     /translation="MLEAAAEKKAENPVALDLRKVAAFTDGFVICTGAQRRQTQAIAD
                     EIVERLRAAGEKASHIEGYERGEWILLDLSDVIVHVFTPETRSFYGLERLWGDTPRIA
                     PTDGARRRAPAKPRPRRVARRA"
     gene            887..1783
                     /locus_tag="VFD06_12875"
     CDS             887..1783
                     /locus_tag="VFD06_12875"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN04468.1"
                     /translation="MARLHDMARRVARTDANVLLTGESGTGKHGLARAIHEASPRAAG
                     PFVTITCANLPAELLESELFGHERGAFTGAHEAREGRFEQAHGGTILIDGVDELAPEL
                     QGKLLRVVQERSFERLAGTRTIHVDVRLLASTRSDLESLVSAGRFRSDLFYRLNVVRL
                     DVPALRDRLEDLAPLAASMLDAIAVKHARPAPRLAPDALEQLAAHDWPGNLRELRNTL
                     EAACIAAAGPEIPAAALPIGSAGGGPVGVAIRDLMTLSQLEERYIREVLRRTHGRRAE
                     AARILGINRKTLLEKRKRYGIP"
     gene            1780..2328
                     /locus_tag="VFD06_12880"
     CDS             1780..2328
                     /locus_tag="VFD06_12880"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZN04469.1"
                     /translation="MKAATNKKKTSPRTVGASRTASPKTPRPVPPARGAARAAAKPLK
                     AAKAVAAPRPAAPPKPRPVSPLNSKQLETYRRLLAERKKALAEAYNKNKTYGRLTEDE
                     GTQDLADKASSAYTKEFLYSLSNTDREVLQKVDESIVRLDTGAFGLCVECQAEINRKR
                     LEAVPWASHCITCQEKIEKGLL"
     gene            2369..>2607
                     /locus_tag="VFD06_12885"
     CDS             2369..>2607
                     /locus_tag="VFD06_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04470.1"
                     /translation="MKIRTVLYLTLGAILVAVLSVLYTTNRAVLDQSLSLGHGITMPV
                     WGCLLLASLLSMLIPILFGLLKDAREFLSTLTSGSR"
BASE COUNT          420 a          951 c          903 g          333 t
ORIGIN      
        1 cacctcgtgg acgtgtcgga gacgaccggg cgcgacccgc tgcacgacat ccaggtcatc
       61 aacggcgagc tgcgcgcctt cagcgaggcg ctcgccgcca agccgcagat cctcgtcgcc
      121 accaagaccg acacgcgccc gtcaccggag cgtctgaaga tggtggagga ggaggcacgc
      181 cggctggggg tgccgttccg cgccatctcc gccgccagcg gcgagggggt caagggcctg
      241 ctcgaggcgg cctggccgct ggtgcaggag gcggcgcagc gtgagacgga gtcggggcac
      301 cgcgagacgg aagcccccgg ggaggaacgg ccattcagct gaagcgcaaa gtcgccctcg
      361 tgctcgaggc ggccgccgag aagaaggcgg agaacccggt cgccctcgac ctccgcaagg
      421 tggccgcctt caccgacggc ttcgtcatct gcaccggcgc gcagcgccgc cagacccagg
      481 cgatcgccga cgagatcgtc gaacgcctgc gggccgccgg cgagaaggcc agccacatcg
      541 aggggtacga acgcggggag tggatcctgc tcgacctgtc ggacgtcatc gtgcatgtct
      601 tcacgccgga gacccggtcg ttctacgggc tcgagcggct gtggggggat accccgcgca
      661 tcgccccgac cgacggcgcg cgccgccgcg cccctgcgaa gccgcgcccc cggcgcgttg
      721 cgcgccgggc ctgaaatccc gccgaccgat ctcgtccgca gggttgacat cggggcatgc
      781 gacctttaaa ttaacgcccc tgctgaaatc gattcgaaag gagccatccg tgggcgcggt
      841 cgcagcattc gcccctcctg ggtttccccc gacgcgttcg gaacgcatgg cgcggctcca
      901 cgacatggcc cgccgcgtgg cgcgcaccga cgccaatgtc ctgctcacgg gggagagcgg
      961 caccggcaag cacggcctgg cgcgggcgat ccacgaggcc agccctcgcg ccgccggccc
     1021 cttcgtcacc atcacctgcg ccaacctgcc ggcggagctc ctcgaaagcg agctgttcgg
     1081 ccacgagagg ggggcgttca ccggcgcgca cgaggcgcgc gaggggcgct tcgagcaggc
     1141 gcacggcggg acgatcctca tcgacggcgt ggacgagctg gcccccgagc tgcaggggaa
     1201 gctgctgcgc gtcgtgcagg agcggtcctt cgagcggctg gccggcaccc gcaccatcca
     1261 cgtcgacgtg cgcctgctgg cctcgacccg gtcggacctc gaatccctgg tgtcggccgg
     1321 gcgcttccgg agcgacctct tttaccgcct caacgtggtc cggctggacg tgccggcgct
     1381 gcgcgaccgg ctcgaggacc tggcgccgct cgccgcgtcg atgctcgacg cgatcgccgt
     1441 caagcacgcg cggccggcgc cgcggctcgc tccggacgcg ctggagcagc tggcggccca
     1501 cgactggccc ggcaacctgc gcgagctgcg caacacgctc gaggccgcct gcatcgcggc
     1561 cgcggggccg gagatcccgg cggcggcgct gccgatcgga tcggccggcg ggggcccggt
     1621 cggcgtggcg atccgcgatc tcatgacgct gtcccagctg gaagaacgtt acatccggga
     1681 ggtcctgcgc cggacgcacg gacgcagggc cgaagccgcc cggatcctcg gaatcaaccg
     1741 caagactctc ctcgagaaac gcaagaggta cggaatccca tgaaggccgc gaccaacaag
     1801 aagaagacct cgccccggac cgtcggggcg tcccgcaccg ccagcccgaa gacgccccgg
     1861 ccggttccgc cggcccgcgg cgccgcgcgc gcggcggcga agccgctcaa ggccgccaag
     1921 gcggtggccg caccacgtcc cgctgccccg ccgaagccgc ggccggtgtc gccgctcaac
     1981 agcaagcagc tcgagaccta ccgccggctc ctggcggagc gcaagaaggc gctcgccgag
     2041 gcgtacaaca agaacaagac gtacggccgg ctgaccgagg acgaggggac ccaggacctc
     2101 gccgacaagg cctcgagcgc ctacaccaag gagttcctgt actcgctgtc caacaccgac
     2161 cgcgaggtgc tgcagaaggt ggacgagtcg atcgtccgcc tcgacaccgg cgccttcggc
     2221 ctgtgcgtcg agtgccaggc ggagatcaac cgcaagcgcc tggaggcggt gccgtgggcg
     2281 agccactgca tcacctgcca ggagaagatt gagaaggggc tcctctaacg aggagggccg
     2341 tctcgctccg cggcgggtcg ggaacatcat gaagatccgc accgtgctgt atctgacgct
     2401 cggcgccatc ctggtcgccg tgctgtcggt cctgtacacc accaaccgag cggtgctcga
     2461 ccagtccctc tcgctcggac acgggatcac catgccggtg tggggctgcc tgctcctggc
     2521 cagcctcctg tcgatgctga tcccgatcct cttcggcctg ctgaaggacg cgcgcgaatt
     2581 cctctcgacg ctcacctccg gcagccg
//
LOCUS       DASRIQ010000525         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_452237, whole genome shotgun sequence.
ACCESSION   DASRIQ010000525 DASRIQ010000000
VERSION     DASRIQ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452237"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFD06_12890"
     CDS             complement(<1..793)
                     /locus_tag="VFD06_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HZN04471.1"
                     /translation="MARPDPHSYFDPAQPRMRRLALDLEVDFDRKVLSGTARLTFAAP
                     SSGRVDLDTKGLTLKSVVTGAGAEVPHTLEPDEPILGRRLRLELPPGTESVVIAYETS
                     PGSTALQWLEPRQTEGRRHPFLFSQCQATHAREVAPVQDSPAVRLTYEAALRVPEPLA
                     AVMSAGPAGIAPGPRPGMRTWRFEMPQPIPPYLLALAVGDLASRDLSPRSRVWAEPLM
                     VERAAWEFAEIEAMLGTAERLFGPYDWDRYDMIVLPPAFPFGGMEN"
     gene            902..2050
                     /locus_tag="VFD06_12895"
     CDS             902..2050
                     /locus_tag="VFD06_12895"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN04472.1"
                     /translation="MGPKREISATGRGTAGASFEAQAREAWRSLFEDLRAKGTVPRDV
                     AVERVFLEDVRAIESARAARAAAYRGAGVDHPPAVSFVEQPPAESGVALIVLAHALPP
                     RRPEDGSPHLLEGLPPGVEGSATRDGETRRIVLTGIAGGEPGDRLDFDAQAARLFARA
                     SSCLMREGLSFRQVARTWIFLADVGRDYGALNRARRAFFETSDVSPAPASTGIGGGPA
                     AAGRLAALDLIAFDGLQPEAMRPLSAPTMNEAPSYGSDFSRGFEVRLADRRLLFVSGT
                     ASIDARGEVLHAGDARAQADRMLKNIERLLGGAGAGFADLSHAVTYVKRPGDEPAVRA
                     ACAAAGLPADLPHVICVAEVCRPEWLCEMEAYGVTAVPAAAGSAAPSS"
     gene            2047..2430
                     /locus_tag="VFD06_12900"
     CDS             2047..2430
                     /locus_tag="VFD06_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HZN04473.1"
                     /translation="MTSHGWLTTGALLGGAAVALGAFGAHGLKARVPADLLVVFETAA
                     RYHLIHALAIVAVAWAAERLPGACVTAAGWLFLSGILIFSGSLYLMVLTGARVLGAVT
                     PIGGLALIAGWIALALAPLLGARAG"
     gene            2430..>2890
                     /locus_tag="VFD06_12905"
     CDS             2430..>2890
                     /locus_tag="VFD06_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZN04474.1"
                     /translation="MHLPDAAASAAAQATDPLLWHRLQFAFTIIYHYLFPQLTMGLAL
                     LVAVMKGLGLRRGGEGWNDAARFWIRIFGLNFAVGVVTGIPMVFQFGTNWAKFSRYAG
                     SVIGQTLAMEGLFAFFLESSFLSLLVWGERRLSRIGHFLAAVALFVGSWLSG"
BASE COUNT          347 a         1058 c         1060 g          425 t
ORIGIN      
        1 ggttctccat gccgccgaag gggaaggcgg gagggagcac gatcatgtcg tagcggtccc
       61 agtcgtaggg accgaagagt cgctcggccg tgccgagcat cgcttcgatc tccgcgaatt
      121 cccacgccgc ccgctccacc atcaacggct ccgcccagac gcgcgagcgc ggcgagagat
      181 cccgcgacgc caggtctccc accgccagcg ccaggaggta cggcgggatc ggctgcggca
      241 tctcgaatcg ccaggtgcgc atgcccgggc gcgggccggg ggcgatcccg gccggccccg
      301 ccgacatgac cgccgccagc ggctccggca cccggagcgc cgcctcgtag gtcaagcgca
      361 ccgcgggact gtcctgaacg ggggccacct cccgcgcgtg ggtcgcctgg cactggctga
      421 acaggaaagg atgccggcgc ccttcggtct ggcgcggctc cagccactgc agcgcggtcg
      481 agcccggcga cgtctcgtac gcgatgacga ccgactcggt gccgggcggc agctcgagtc
      541 gcaggcggcg gccgaggatc ggctcgtccg gctcgagcgt gtgcgggacc tccgcaccgg
      601 cgccggtgac cacgctcttg agcgtcagcc ccttggtgtc gagatcgacc cgccccgacg
      661 agggggcggc gaaggtcagg cgcgccgtcc cggacagcac cttgcggtcg aaatcgacct
      721 cgagatcgag ggcgagccgc cgcatgcgcg gctgcgccgg atcgaagtac gagtgcgggt
      781 cgggccgggc cacgtgcgtc cctcgggtcg cgtgacgtta cctcagcccg ccgccggcgc
      841 acaagcgtgg gtccgggccc ccgcgcacga ggcgcaggtc gctgtatagt ctggacttcc
      901 gatgggtccg aagcgggaga tctccgcgac cggacggggg accgccgggg cctcgttcga
      961 ggcgcaggcg cgcgaggcct ggcgcagcct cttcgaggac ctgcgcgcga agggcacggt
     1021 cccgcgcgac gtcgcggtcg agcgagtctt tctcgaggac gtgcgcgcca tcgaatcggc
     1081 gcgcgcggcc cgcgccgcgg cctaccgcgg cgccggcgtg gatcaccccc cggccgtctc
     1141 gttcgtggag cagccgccag cggaatccgg agtcgccctc atcgtcctgg cgcacgccct
     1201 ccccccgcgg cgcccggagg acggcagccc gcaccttctc gaagggctgc ccccgggggt
     1261 cgaaggctcg gcgacgcgcg acggcgagac gcggcgcatc gtcctcaccg ggatcgccgg
     1321 gggggagccg ggagaccggc tcgacttcga tgcgcaggcg gcgcgcctgt tcgcccgcgc
     1381 ctcctcctgc ctgatgcggg aggggctctc cttccggcag gtggcgcgca cctggatctt
     1441 cctggcggac gtcgggcgcg actacggcgc gctgaaccgg gcgcgacgcg ccttcttcga
     1501 gacgtcggac gtcagccccg ccccggcgag caccggcatc ggcgggggcc cggccgccgc
     1561 cggccggctg gcggctctcg acctcatcgc cttcgacggc ctgcagccgg aggcgatgcg
     1621 cccgctgagc gcgccgacga tgaacgaggc gccgtcgtac ggatccgatt tctcgcgcgg
     1681 cttcgaggtg cggctcgcgg accggaggct cctgttcgtc tccggaaccg cgagcatcga
     1741 cgcgcggggc gaggtgctgc acgcggggga cgcccgggcg caggcggatc gcatgctgaa
     1801 gaacatcgag cggctgctcg gcggcgccgg cgccggtttc gcggatctga gccacgccgt
     1861 cacctacgtc aagcgccccg gagacgagcc cgcggtccgg gcggcctgtg ccgcggccgg
     1921 cctgccggcc gacctgccgc acgtcatctg cgtcgcggag gtgtgccggc cggagtggct
     1981 ctgcgagatg gaggcctacg gcgtgacggc cgtcccggcc gcggccggga gcgcggcgcc
     2041 gtcctcgtga cctcgcacgg ctggctgacg accggcgcgc tcctcggcgg cgcggcggtg
     2101 gcgctcggcg ccttcggcgc gcatggtctc aaggcgcgcg tgccggccga tctcctcgtc
     2161 gtcttcgaga ccgcggcgcg ctaccacctg atccacgcgc tggcgatcgt ggcggtcgcg
     2221 tgggcggccg aacggcttcc tggcgcgtgc gtcaccgccg ccggatggct gttcctctcc
     2281 ggcatcctga tcttctccgg ctcgctctac ctcatggtcc tgaccggcgc gcgggtcctg
     2341 ggcgccgtca ctccgatcgg cgggctggcc ctcatcgcag gctggatcgc cctggcgctc
     2401 gcgcctctcc tcggagcccg cgccggatga tgcatcttcc cgacgccgcg gcgtccgccg
     2461 ccgctcaggc gaccgatccg ctgctctggc accggctgca gttcgcgttc accatcatct
     2521 atcactacct cttcccgcag ctgacgatgg ggctggcgct gctcgtcgcg gtcatgaagg
     2581 ggctcgggct gcggcgcggc ggggaaggct ggaacgacgc cgcccgcttc tggatccgga
     2641 tcttcggcct gaacttcgcc gtcggcgtgg tgaccggcat ccccatggtg ttccagttcg
     2701 gcacgaactg ggcgaagttc agccggtacg cggggagcgt gatcggccag accctggcga
     2761 tggagggact cttcgccttc ttcctcgaat cgagcttcct gtccctcctc gtctggggtg
     2821 agcggcgact ctcgaggatc ggccacttct tggcggcggt ggccctcttt gtcgggagct
     2881 ggctctccgg
//
LOCUS       DASRIQ010000526         5189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3880478, whole genome shotgun sequence.
ACCESSION   DASRIQ010000526 DASRIQ010000000
VERSION     DASRIQ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3880478"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="VFD06_12910"
     CDS             complement(<1..2226)
                     /locus_tag="VFD06_12910"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN04475.1"
                     /translation="MHRRLSTTILILAAALAASASRDAFALPANFTESVAFSGLNLPI
                     AVRFSPDGRVFVAEKSGNIKVFDGLSDATATMVVGPGTSYDLRPKVHDFWDRGLLGFA
                     LHPNFPSTPYLYVLYTYDYDPLDPSRPAPRWGDTCPDRDASNNIIGPGSTDDGCVVNG
                     RLSRFEVGANNVLIGGETVILENNWCQQYPSHSIGSLVFGQEGALYVTAGDGASFTTE
                     DWGQDFTTTPLRIDPNTGQRYTPINPCADPNAPRGTGTTKPNAAGGALRSQDVRTLGA
                     GDPVTFDGSVLRIDPNTGAAWPDNPFVGGATTEDDRIIAHGLRNPFRATRRPGTNEIW
                     VGDVGWFTWEEINRITNPSDATVENFGWPCVEGPDRPAGYNNNLSLCTSLATASTTQG
                     HYAYHHGEPIYAGDPCGTASSSVSGLAFYNGGTYPSSWNGALFFADHSRNCIFVMTAG
                     AGGLPDPATRTVFVSHDSNAQQTGDPDPVDLTIGPGGDLFIVDFEDGNIRRIRYNTSN
                     NPPTAAMQATPSSGMVPLLVQFDGTGSSDPDPGSTLFHAWDLDGDGQYDDAFVPQPVW
                     TYTQIGNVTARLRVTDDDNATATTQKTISVGNSPPTATIATPAASLTWRVGEVIHFSG
                     TGSDPTDGALPATAFRWDVVLHHCPGGPGGCHEHDVESFDDIASGPYTAPDHDYYSYL
                     EFRLTVTDSGGLSDTDSVSLEPEPVTNSFTSTPSGLTFTVAGIDAVTPFTRQSIINST
                     IS"
     gene            2882..3814
                     /locus_tag="VFD06_12915"
     CDS             2882..3814
                     /locus_tag="VFD06_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04476.1"
                     /translation="MSATCGSANCFLVTGTQEGTVPKGQWVFDLSFRYLPQDRKLEGR
                     DEVAEVLAPAIDFENESIEPDHHREFTTLNELMQIDLSYGATPRLSVAAALPILNRRY
                     HEHDDGVGTPEEAFSNSAGTTGFGDVRLGVRAALLTRPRHQMVGSLGLELPTGPYRLR
                     DDEGEIGEPTLMPGSGSYDVVAGLLYSHLFGESGWNLFASGTFRENGENPLDYRLGAE
                     QVYAVGIEKRAGGTFLWSVQANGRRTGRDEYLGERVPSTGATLVNVTPGLRVSNAEQD
                     FAFYAFAQVPVLQEVNEAQLAPRYGLILGVSKTF"
     gene            complement(3833..>5189)
                     /locus_tag="VFD06_12920"
     CDS             complement(3833..>5189)
                     /locus_tag="VFD06_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN04477.1"
                     /translation="NPPSKTPLLTLLKQARAFGLGVVVATQNPVDLDYKGLSNAGTWF
                     LGRLQTERDKARVIEGLEGASSARGAAFDRASVERTLAGLKSRVFLMSNAHEDAPALM
                     QTRWTLSYLRGPLTRPQIQGLMASRTSAPAAPAAAAPGDSAPANSRTSAPPAGQEDRP
                     VVPPDIPQVFAAAAPGAVLRPAILGEAKLHYVDARAGIDLWETARLLVPLGPDHPAPT
                     WSEARDAVSGPPLGPATPASARFAAVPGPALRPKSYADWSRTLADHLYQGRTVTFLRA
                     PDLKAVSRPGESAAEFAVRLRDLVRERRDAAIETLRAKYAPRLAGLQERLRRADQRVE
                     RERDQFQQQTVQTAISVGATVLGALFGRKITSTGTLGRATTAMRGAGRSMREREEIGQ
                     AQETAEAVRRQIADLEEELRSEIARLQADGDPATQEIAVRPRKADIVVERLLLAWIPV
                     D"
BASE COUNT          694 a         1719 c         1939 g          837 t
ORIGIN      
        1 tgagatcgtg gagttgatga tcgactggcg ggtgaagggg gtcacggcgt cgatcccggc
       61 gaccgtgaag gtcagcccgg acggcgtcga ggtgaaggag ttggtgaccg gctccggctc
      121 cagcgacacg ctgtcggtgt cgctgaggcc gcccgagtcg gtgacggtga ggcggaactc
      181 caggtacgag tagtagtcgt ggtccggggc ggtgtacggg ccggaggcga tgtcgtcgaa
      241 cgactcgacg tcgtgctcgt ggcagccgcc ggggcctccg ggacagtggt gcagcaccac
      301 gtcccagcgg aacgcggtcg cggggagcgc gccgtcggtc ggatcgctcc cggtcccgga
      361 gaagtggatc acctcgccga cgcgccaggt gagcgacgcg gccggcgtgg cgatggtcgc
      421 ggtcggcggg gagttgccga cggagatcgt cttctgggtg gtggcggtgg cgttgtcgtc
      481 gtcggtgacc cgcaggcgcg cggtgacgtt gccgatctgc gtgtaggtcc agaccggctg
      541 cggcacgaac gcgtcgtcgt actgcccgtc gccgtcgagg tcccaggcgt ggaagagagt
      601 gctgcccgga tccggatcgg aggagccggt gccgtcgaac tgcaccagga gcggcaccat
      661 gccgctcgaa ggggtcgcct gcatcgccgc ggtcggcggg ttgttgctgg tgttgtaccg
      721 gatgcggcgg atgttgccgt cctcgaagtc gacgatgaac aggtccccac ccggcccgat
      781 cgtcaggtcc accggatccg ggtcgcccgt ctgctgcgcg ttgctgtcgt gcgacacgaa
      841 gacggtgcgg gtggccggat cgggcaggcc gccggcaccg gcggtcatca cgaagatgca
      901 gttgcgcgag tggtcggcga agaacagcgc cccgttccac gacgacggat aggtgccccc
      961 gttgtagaag gcgagaccgg agaccgacga gctggcggtg ccgcacggat cgccggcata
     1021 gatcggctcg ccgtggtggt aggcgtaatg accctgcgtc gtgctggcgg tggcgagcga
     1081 cgtgcagagg ctcaggttgt tgttgtagcc ggcgggccgg tccggcccct cgacgcaggg
     1141 ccagccgaag ttctccaccg tcgcgtccga cgggttcgtg atgcggttga tctcctccca
     1201 ggtgaaccac cccacgtcgc cgacccagat ctcgttcgtg ccggggcggc gcgtggcgcg
     1261 gaacgggttg cgcaggccgt gggcgatgat ccgatcgtct tcggtcgtgg cgccgccgac
     1321 gaagggattg tcgggccagg cggctccggt gttcggatcg atgcgcagca ccgacccgtc
     1381 gaacgtgacc gggtcgccgg cgcccagcgt ccggacgtcc tgcgagcgca gcgcccctcc
     1441 ggcggcgttc ggcttggtgg tgccggtgcc gcgcggggcg ttcgggtcgg cgcacgggtt
     1501 gatcggcgtg tagcgctgcc cggtgttcgg gtcgatgcga agaggggtgg tggtgaagtc
     1561 ctgcccccag tcctcggtcg tgaacgaggc gccgtcgccg gcggtcacgt agagcgctcc
     1621 ttcctgtccg aacaccagcg agccgatcga atggctcggg tactgctggc accagttgtt
     1681 ctcgaggatc accgtctcgc cgccgatcag cacgttgttg gcgccgacct cgaagcggga
     1741 gaggcgaccg ttgacgacgc agccgtcgtc ggtcgagccg gggccgatga tgttgttgct
     1801 cgcgtcgcgg tcggggcagg tgtcccccca gcgcggcgcc ggacgggacg gatcgagcgg
     1861 gtcgtaatcg taggtgtaca gcacgtagag atacggcgtg gacgggaagt tcgggtgcag
     1921 cgcgaacccg agcagccccc ggtcccagaa gtcgtgcacc ttgggacgca ggtcgtacga
     1981 ggttcccgga ccgaccacca tcgtggcggt cgcgtcgctc aggccatcga acaccttgat
     2041 gttgccgctc ttctccgcga cgaaaacccg cccgtcgggc gagaagcgga ccgcaatcgg
     2101 aaggttcagt ccggagaagg cgacgctctc ggtgaaattc gcgggcaggg cgaaggcgtc
     2161 gcgcgatgcg gaggcggcca gcgcggccgc caggatcaga atggtggtgg agagacgacg
     2221 atgcacggcg cgaccccctc gtgcctggtg cttgaaatgt ggtgttggtt tgttcccggg
     2281 ttccccggcc cggtgttctt ctggtacggc gaggtgcccg ggaggtcaag gggattcggg
     2341 ggcggtccat ggggcggtca cgattccgtc attggagtac agtttcttcc gtcatgccgc
     2401 gcgccctgcg cctcctcctt gcgaccgtcc tcctcgcgac ggtggctgcg ccgctcctcc
     2461 cggtgccggc cgccgccgga ccgggatgcg cgatgtgctc ccgcacctgc tgctgcaggc
     2521 cgccgggggc ccgcgaggcg tgccggctgt cccgtccctg cgggtcgccg ggagaggacg
     2581 cggcgccggc ggcgcagagc tcttcacgtc ccgccctgct cgacgcgatc ccccctggcg
     2641 ccgctcctcc actcctcatc gccgcggcac cgttcccttt ctctgggctg ccggtcgcga
     2701 ccgcagaccc gccccccgtc ccgccgcctc gttccttctc cacctgctga tccggcccgt
     2761 cgttttcaac gtctgatcag gactccgtcg aggggtgtgg aggtgcgtgt gtatgcggtc
     2821 gcttcgatcc cgcgcccatt gggcgctcgc agttgtcgtg cttttgattc cggcggcgcc
     2881 ggtgtccgcg acgtgcggct ccgccaattg ttttctcgtc accgggaccc aggaggggac
     2941 cgtcccgaag ggacagtggg tgttcgacct gtcgttccgt tatctgccgc aggaccggaa
     3001 actcgagggc cgcgacgagg tggccgaggt tctcgcaccg gccatcgact tcgagaacga
     3061 gtcgatcgag ccggatcacc atcgcgagtt cacgacgctc aacgagctga tgcagatcga
     3121 tctgtcgtac ggagcgaccc cccggctcag cgtcgcggcg gcgctgccga tcctgaatcg
     3181 ccgctaccac gagcacgacg acggcgtcgg cacgccggag gaggcctttt cgaactcggc
     3241 cggcacgacc gggttcggcg acgtgcgcct gggggtgcgg gcggctctgc tcacgcggcc
     3301 gcggcaccag atggtcggat cgctggggct cgagctgccg accggaccgt accgtctgcg
     3361 tgacgacgag ggggagatcg gcgagccgac cctcatgccg gggagcggct cgtacgatgt
     3421 cgtcgccggt cttctctatt cgcacctgtt cggcgagagc ggctggaacc tgttcgcctc
     3481 ggggacgttc cgggagaacg gcgagaaccc gctggactat cgcctcgggg cggagcaagt
     3541 gtacgcggtc ggcatcgaga agcgcgccgg cgggaccttc ctctggtcgg tgcaggcgaa
     3601 cgggcgccgg accgggcggg acgagtacct cggggagagg gtcccctcca ccggcgcgac
     3661 gctcgtcaac gtgacgcccg gtctgcgcgt cagcaacgcc gagcaggact tcgcgttcta
     3721 tgccttcgcg caggtgccgg tcctccagga ggtgaacgaa gcgcagctgg cgccgcgcta
     3781 cggcctcatc ctcggcgtgt cgaagacctt ctagccggcg gcgagggctc gcctagtcga
     3841 ccgggatcca ggcgaggagg aggcgctcga cgacgatgtc cgccttgcgg gggcggacgg
     3901 cgatctcctg ggtggcggga tcgccgtccg cctgcaggcg ggcgatctcc gacctcagct
     3961 cctcttcgag gtcggcgatc tgccggcgca ccgcctcggc ggtctcctgc gcctggccga
     4021 tctcctcgcg ctcgcgcatc gagcggccgg cgccgcgcat cgcggtcgtg gcccggccga
     4081 gggttccggt gctcgtgatc ttccgtccga acagggctcc gaggacggtc gcgcccaccg
     4141 agatcgcggt ctgcaccgtc tgctgctgga actgatcccg ctcgcgctcg acccgctggt
     4201 cggcccgccg cagcctctcc tggagccccg cgagccgcgg cgcgtacttc gccctcagcg
     4261 tctcgatcgc ggcgtcgcgg cgctcgcgga cgagatcgcg caggcgcacc gcgaattccg
     4321 cggcgctctc gccgggccgg gagaccgcct tgagatccgg cgcgcgcagg aaggtcacgg
     4381 tgcgcccctg gtagaggtga tcggccaggg tgcggctcca gtcggcgtac gacttcggcc
     4441 gcagcgccgg gccggggacg gcggcgaagc gggccgacgc gggcgtcgcc ggaccgagcg
     4501 gagggccgga gacggcgtcg cgcgcctcgc tccaggtcgg cgcgggatga tcggggccga
     4561 gcggcacgag gagacgcgcc gtctcccaca gatcgatccc cgccctggcg tcgacgtaat
     4621 gaagcttcgc ttcgccgagg atcgccgggc gcagcacagc accgggggca gcggccgcga
     4681 acacctgtgg gatgtcgggc ggaaccaccg gccgatcctc ctgacccgcc ggcggcgcgg
     4741 atgtccggga gttcgcaggg gcggaatctc cgggcgcggc cgccgccggt gcggccggtg
     4801 cggaggtccg ggaggccatg agcccctgga tctgcggccg tgtgaggggg ccccgcaggt
     4861 acgagagggt ccagcgcgtc tgcatcaggg cgggtgcatc ctcgtgggcg ttgctcatga
     4921 ggaagacgcg gctcttcagg ccggcgaggg tgcgctcgac ggaggcgcga tcgaacgccg
     4981 caccgcgcgc cgacgacgcc ccctcgagcc cctcgatgac gcgcgccttg tcccgttcgg
     5041 tctgcaggcg tccgaggaac cacgtccccg cgttcgacag ccccttgtag tcgaggtcca
     5101 ccgggttctg ggtcgccacc accacgccca gcccgaaggc gcgcgcttgc ttgagcaggg
     5161 tcaggagcgg cgtcttcgac ggcgggttc
//
LOCUS       DASRIQ010000527         5227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2831054, whole genome shotgun sequence.
ACCESSION   DASRIQ010000527 DASRIQ010000000
VERSION     DASRIQ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2831054"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(517..3687)
                     /locus_tag="VFD06_12925"
     CDS             complement(517..3687)
                     /locus_tag="VFD06_12925"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HZN04478.1"
                     /translation="MKSRSIHTFVLLALVLLAGDVSAAPSSDPRVSALKALRAASGAP
                     LTGSTEAGAVRLVGCDVDVPGATPVQQARSFLAAYGAAFELTGPDQQLGLRGLRSQGS
                     LNVVSFAQTYKRVPVFAGEIRVGVKAAAVAGMGRVVFAGGAILPDQARFGGLDTDASV
                     APAACVEAARAHLGRAGAGTLADPKLMIYDARLLGGAPGPHLVWALTLGGGDPRQVFC
                     DAQTAAVVFDRAFAAEELDLWMGLQYPNPSHTYIGDEDGLEPEGQSNTDAVLEFGYIH
                     TVYDFFADLFGWRGTAGNDNQLQLLVDSDTGRCGRFYWDPFGEAIQIDTGCAGFDVTA
                     HEYTHGIINHSSGLVYNGIPGAINESYSDTMGVFVDDQDWLLGEDKSGDPGNPIRNFQ
                     SPPSEQTCSPCVPQPDRLSMLCTEDDDCNYSGDSNGVHTNSGILNKAHYLIAAGGDFN
                     GRPPFTGIGRFKMGVLTFLTMHVVPSGASFQDVRQYSIETAKTLAIDGQAGFVPSDVC
                     VVRNAFASVEIGPGDFDCDGIEDNWQDPDGDFLPTSMDNCPAESNPEQADLDDDGIGN
                     HCDGDFDNDGIPNGFDNCNFDSNWDQLDADQDGVGNACDFDSDNDGIKDNGDASGADN
                     PCANGDTEWCDDNCRFEPNPDQIDSNNDGFGDACDPDLDTDGLFVLDDNCTFVNNPEQ
                     QDTDGDGLGDACDKCPDVADWDGTYTLKKWIMQTPVPYQPDSDGDGTPDACDRIAFDR
                     SGLELGGASYNPARIVRPGDGRKSAVVTGPEDTRFRIPLPLCDPDGDPDASLVTEVVF
                     VDLDATVDGVLYDDDGLALGSLRPGPTGSSTRGLRVTPDCARTYFLEISLGRGFAGSD
                     AFAVESLLVPSSSPNPWRTPGSDLPPPGSSRDGDGDGLPDFIDSCPTVADPTGADIDA
                     DGVGDACDNCSADANSQQEDVDGDGHGDVCDCAPADAGAFAPPGEVSGVAFDADGMTL
                     RWDPAAAGPATVYDVLRGEGGGPVGGPGAEACLAQQTPGTSFPDPTLPADGQMFWYVV
                     RARNTCGVGTYGQQWNGTPSLSLTCP"
     gene            3877..4461
                     /locus_tag="VFD06_12930"
     CDS             3877..4461
                     /locus_tag="VFD06_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZN04479.1"
                     /translation="MPEPGEVTRLLRAYEAGDPQAFQRLVPLVYDELRRLAREQLRRR
                     GPAWPSLGATDLVHETYLKLAGAESLTAADRGHLIAIAATAMRQVLVDRARARLRAKR
                     GGGDTPVALEEEDAATGPPPEWLIDLDRALTQLRKRDERVAAVFECRFFADLGEEETA
                     EALGIPLRTAQRDWMRARAWLRAALRATGPDADA"
     gene            4454..>5227
                     /locus_tag="VFD06_12935"
     CDS             4454..>5227
                     /locus_tag="VFD06_12935"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN04480.1"
                     /translation="MPEGSDDWKQVDALLQEALDRPLEERERWLEEACAGNAALRDRV
                     AGLLHLAAAEDDGLTQGGALRVPRQGGVPEARALSPGDHLGRYEIRSLLGAGGMGRVY
                     RALDPVLGREVAIKALGGNALRADSGSLRRFEREARLLARMSHPNIATIHGFELHDGA
                     PYLILERVEGQTLAERLRNGPVPLAEALTIAVQVAEGLAEAHGKGVIHRDLKPANVML
                     TSNGRVKLLDFGLAKRADPGGVGDSVTVNPTTGAGEILGT"
BASE COUNT          819 a         1771 c         1826 g          811 t
ORIGIN      
        1 gccgtgacgg ttttctgggg cgctcccgag agctttacgt tgatgatcgc cgtgcctccc
       61 tccgggacgg agaccgggtc ggcctccacg acgaaggtca cgttgttggc gtagacgagc
      121 gaccctccgg gcgcgcccag ggcgccggcg agcggaagga ttgccaatac ggaaaggaat
      181 cggccgaccg gtttcatgca cccctcccgc cctgtgcgag cgcctcgctt cgacgcaccg
      241 cgacgaaggc acttgctgca agaaccacaa cgtagcgaga tggcggcacc ctacggctgc
      301 attggaaagg cgtcaatcag caagattgcg gagggatcat tccgagactt cgattccgcg
      361 accggcctgg cgtcccgaca cgcgtgacgt gcgaaagggt ccgcgtcggc agaggtcgga
      421 cgggacagga ccgcgaggcc cttcctcagc tcgtccctgg ctccgtgagc ctcgtcgctc
      481 aagctcacgc cgctcctgga gcggaatgcg ccctgctcac gggcaggtca ggctgaggct
      541 cggggtgccg ttccactgct ggccgtatgt ccccaccccg caggtgttcc gggcgcgcac
      601 cacgtaccag aacatctggc catccgccgg cagcgtcggg tcggggaacg acgtgccggg
      661 cgtctgctgg gcgaggcagg cctccgcccc gggaccgccg acagggcctc cgccctcccc
      721 gcgcagcacg tcatagaccg tcgccggtcc cgcggccgcg gggtcccagc gaagcgtcat
      781 tccgtccgcg tcgaacgcga ccccggaaac ctcgcccggc ggcgcgaagg cgcccgcatc
      841 ggccggagcg cagtcgcaga cgtccccatg gccgtcacca tccacgtcct cctgctggga
      901 attggcgtcc gcactgcagt tgtcgcaggc atcgccgacg ccgtccgcgt cgatgtcggc
      961 accggtcgga tcggcgaccg tcgggcagga gtcgatgaag tccggcaggc cgtcgccgtc
     1021 gccatcgcgg ctcgagcccg gtggagggag gtcggagccc ggcgtcctcc aggggttcgg
     1081 actcgacgac ggaaccagta aggattccac ggcgaacgcg tccgagcccg cgaacccccg
     1141 acccagcgaa atctccagaa agtaggtgcg cgcgcagtcc ggcgtcacgc gcaacccccg
     1201 cgtgctggac cccgtcggcc ccgggcggag cgatccgagg gccaggccgt cgtcgtcgta
     1261 gagaacgccg tccacagtcg cgtcgaggtc aacgaagacg acctcggtca ccagcgacgc
     1321 gtcggggtcc ccatcgggat cacagagcgg cagggggatc ctgaaacggg tgtcctccgg
     1381 gccggtgacg acagcagact ttcgcccgtc gccggggcgg acaatccgcg ctgggttgta
     1441 ggaggcgccg cccagctcga gcccggaccg gtcgaaagcg atcctgtcgc aggcgtcggg
     1501 agtgccgtcg ccgtcgctgt ccggctggta cgggaccggc gtctgcatga tccatttctt
     1561 caaagtgtaa gtgccgtccc agtcggccac gtccgggcac ttgtcgcagg cgtcgccgag
     1621 gccgtcaccg tccgtgtcct gctgctccgg gttgttgacg aatgtgcagt tgtcgtccag
     1681 gacgaacagg ccgtcggtgt caaggtcggg atcgcaggcg tcgccgaatc cgtcgttgtt
     1741 gctgtcgatc tgatccgggt tgggctcgaa gcggcagttg tcgtcgcacc actccgtgtc
     1801 gccgttggcg cacgggttgt ccgcgccgct ggcgtcgccg ttgtccttga tcccgtcgtt
     1861 gtcgctgtcg aagtcgcagg cattccccac cccgtcctgg tcggcatcga gctggtccca
     1921 gttcgagtca aagttgcagt tgtcaaagcc gttgggaatg ccgtcgttgt caaaatcgcc
     1981 gtcgcagtgg ttgccgatgc cgtcatcgtc aaggtcggcc tgctcagggt tcgattccgc
     2041 tggacaattg tccatcgagg tcggcaggaa gtcgccgtcc gggtcctgcc agttgtcttc
     2101 gatcccgtcg cagtcgaagt cgccggggcc gatctcgacg gaggcgaagg cattcctgac
     2161 cacgcagacg tccgacggaa cgaatccggc ctggccgtcg atcgccaacg tctttgccgt
     2221 ctcgatcgag tattgacgga cgtcctggaa ggatgcgccg gaggggacga cgtgcatcgt
     2281 gagaaacgtg agaaccccca tcttgaagcg gccaatcccc gtgaatggag gtctgccgtt
     2341 gaagtcgccg ccggccgcga tcaggtaatg cgccttgttc aagatcccgc tgttcgtgtg
     2401 cacgccgttg ctgtccccgc tgtaattgca atcgtcgtcc tcggtgcaca gcatcgagag
     2461 ccggtcgggt tgcggaacac aggggctgca cgtctgttcg ctgggaggcg actggaaatt
     2521 acggatcggg ttgcccgggt ctccgctctt gtcctcgccg agcaaccagt cctggtcgtc
     2581 gacgaagacc cccatggtgt cggagtacga ctcgttgatg gcgcccggga tgccgttgta
     2641 caccaggccg ctgctgtgat tgatgatccc gtgcgtgtat tcgtgcgcgg tgacgtcgaa
     2701 gccggcgcag cccgtgtcga tctgaatcgc ctcgccgaag gggtcccagt agaaccgtcc
     2761 gcaacggcct gtgtcgctgt ccacgaggag ctgcagctgg ttgtcgttcc cggccgtgcc
     2821 gcgccatccg aagagatccg cgaaaaaatc gtagacggtg tggatgtatc caaactcgag
     2881 caccgcgtcg gtgttcgact ggccctccgg ctcgagtccg tcttcgtcgc caatgtacgt
     2941 gtgggacggg ttcgggtact gcagccccat ccagaggtcg agttcctcgg cggcgaaggc
     3001 ccggtcgaac acgacggcgg cggtctgggc gtcgcagaag acctgcctgg gatcgccgcc
     3061 gcccagcgtc agcgcccaga cgaggtgggg gcccggcgcc ccgccgagga ggcgggcgtc
     3121 gtagatcatc agcttggggt cggcgagcgt cccggcccct gcgcgtccca ggtgggcgcg
     3181 cgcggcctcg acgcacgccg caggcgcgac cgaggcgtca gtgtcgaggc ccccgaagcg
     3241 cgcttgatcc ggcaggatgg cgccgccggc aaagaccacc cggcccatcc ccgccacggc
     3301 ggcggccttc acgccgacgc gaatctcgcc cgcgaacacc ggcacgcgct tgtaggtctg
     3361 cgcgaaggag acgacgttca gcgatccctg ggaacgcagc ccgcgcagtc ccagttgctg
     3421 atccggtccc gtcagctcga aggcggcgcc gtaagccgcc aggaaggatc gggcctgctg
     3481 gaccggggtc gccccgggca catcgacgtc gcagccgacc aggcgcacag cccccgcctc
     3541 cgtgctgccg gtgagcggcg ccccggacgc ggcgcggagg gccttcaggg cactgacccg
     3601 cgggtcggag gacggggcgg cggagacgtc cccggccagc agaacaaggg caagcaggac
     3661 aaaggtgtgg atcgatcggc tcttcatggc gaaatcccct ctgtggctcg gctccagccc
     3721 tcgggctgcg cgagccggga gcgaccgtga ctggccgcca tgccccatac acgacaacca
     3781 cgagggaaac ccgccggccg ggcgccactt tcctatgcct ccgttgacgg ggggggaggt
     3841 atattctgga gacaccgtct cccacaggtg aggtgagtgc ccgaaccagg cgaggtcaca
     3901 cggctgctga gagcctacga ggcgggcgac ccccaggcct tccagcgcct cgtccccctg
     3961 gtctacgacg agctgcggcg cctggcccgg gagcagcttc gccgcagggg gccggcctgg
     4021 ccctctctcg gcgccaccga tctcgtccat gagacgtacc tcaaactcgc gggcgctgag
     4081 agcctcacag ccgccgaccg tggacacctg atcgccatcg ctgccaccgc catgcgccag
     4141 gttctcgtcg atcgggcccg cgcgcgcctg cgggccaagc gcggaggcgg cgacacgccc
     4201 gtggcgctgg aggaggagga tgcggcgacc gggccgccgc cggagtggct gatcgacctc
     4261 gaccgcgccc tcacgcagtt gcgcaagcgc gatgagcgcg tggcggccgt gttcgagtgc
     4321 cgcttcttcg cggaccttgg agaggaggag accgccgagg ctctgggcat cccgctgcgg
     4381 accgcgcagc gggactggat gcgggcgcgg gcctggctgc gagccgcgct ccgggcgacg
     4441 gggccggacg ccgatgcctg aaggttccga cgactggaaa caggtcgacg ccctgctgca
     4501 ggaagcgctc gaccggcccc tcgaggagcg agagcgctgg ctggaggagg cttgcgccgg
     4561 gaacgcggcg ctccgggacc gcgtcgcggg gctcctccac ctcgcagcgg cggaggacga
     4621 cgggctgacg caggggggcg cgctccgcgt tccgcgtcag gggggcgtgc cggaggccag
     4681 ggccctgagc ccgggggacc acctggggcg gtacgagatc cggtcccttc tcggcgcggg
     4741 cggcatgggg cgcgtctacc gcgccctcga cccggtgctg ggccgggaag tcgccatcaa
     4801 ggccctgggt ggcaacgctc ttcgtgcaga ctcgggaagc ctccggcgtt tcgagcgcga
     4861 ggcccgcctc cttgccagga tgagtcaccc caacatcgcc accatccacg gtttcgagtt
     4921 gcacgacggc gccccctatc tcatcctgga gcgcgtcgaa ggccagacgc tggcggagag
     4981 gctccggaac gggccggtgc ccctcgccga ggccctgacg attgcagttc aggtggcgga
     5041 agggctcgca gaagcccacg gcaagggcgt gatccaccgc gacctcaagc cggcaaacgt
     5101 gatgctgaca tcgaacggac gcgtgaagct gctcgacttc ggattggcca agcgggcgga
     5161 tccagggggc gtcggggact ccgtcaccgt gaacccgacg acgggcgcag gagagattct
     5221 cggaacc
//
LOCUS       DASRIQ010000528         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3317940, whole genome shotgun sequence.
ACCESSION   DASRIQ010000528 DASRIQ010000000
VERSION     DASRIQ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3317940"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="VFD06_12940"
     CDS             <1..108
                     /locus_tag="VFD06_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HZN04481.1"
                     /translation="RLEKAGAHVEVFAGDEICRKGSGGPTCLTRPILRS"
     gene            complement(204..1748)
                     /locus_tag="VFD06_12945"
     CDS             complement(204..1748)
                     /locus_tag="VFD06_12945"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZN04482.1"
                     /translation="MIVLARISCSLRRAAPALVVAAGAFLAAYGLAAATGRSAPPGGN
                     PGQANIDQRLALVLAREGFTGRVESTLAARLGRPIDPARADLGRLLFFDKILGLHDDN
                     SCAGCHAPAFGFGDSQRMAIGVQNNDVVGPSRHGPRNQRRSPLVANTVFYPALMWTPR
                     FVALSGDPFDASLGFQFPPPENVVTGVPTLLSAQGSLPSTELVEMAGFNGITANPGPL
                     GPRHFQFDDGQGQVLPAPDGTGVHNFPIQAAVDARLNANALYLQKFGEVFNGGVPLAA
                     GAITIDMRRLAIAEFQANLTAANAPLDRFARGEPGAMTIGQKRGALLFFGEAGCVSCH
                     AVAGPANEMFSDFQPHRIGGPQVAPVFGVGTGNVIFDGPDENEDFGFEQTTGDPALRY
                     MFRTAPLRNLKVSPGFFHNGAFLSLEAAIAHHLDVDRSLRQYDPAANALPADLSVGPF
                     MGILHAGIDPRLRKAVRLDDQEVRDLVEFVRDGLFDARVLSFCDQLPASVPSGMPLQE
                     FESCGG"
     gene            complement(2153..3019)
                     /locus_tag="VFD06_12950"
     CDS             complement(2153..3019)
                     /locus_tag="VFD06_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04483.1"
                     /translation="MSPPRRAIGFLAGIGRLALAAWMMGAAAGAVMCGEPVSGRLDPD
                     PRAQAAIEETVSAIVRNDEAAADRFAALQRLGERDRTALLLQMALYLEGAEGTDRGMA
                     GAIVLRRIGFTPKETIEAVGPSLEDAAPALRRVLAGMLATVDRRDGGEADFRVYDAWL
                     AGRGGRPPAAFVAYLYDVSPDEAVLLMQRVYGRAAPPPPGSMRSRDELKSIVTSRDVS
                     RPLKEEERARAAAALATLSADPAWWVRRYAATVLRDDAALGTSAILERLKEDPDLLVR
                     DGLTHPPGARGD"
     gene            complement(3024..>3256)
                     /locus_tag="VFD06_12955"
     CDS             complement(3024..>3256)
                     /locus_tag="VFD06_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04484.1"
                     /translation="IDPETALPPTTAYHANYYEHPGCGFLQLCGGPVPGANLNVNVTA
                     TPWGSGLTHFTIDDAAEVLQGLLDLMVIHVPY"
BASE COUNT          476 a         1134 c         1191 g          455 t
ORIGIN      
        1 cgcctggaga aagcgggggc ccatgtcgag gtgttcgccg gcgacgagat ctgcaggaaa
       61 gggtcgggag gccccacctg cctgacgcgg ccgatcctga gatcctgaga cagcctgctg
      121 cttcgcctat ccgccggcgc agccggtccc cgccgccgcc acccgcggct tgtcggtcgt
      181 atcgctgccc aggaccacgg acctcagccg ccgcagctct cgaactcctg caggggcatg
      241 ccgctcggca ccgacgccgg cagctggtcg cagaacgaca ggacgcgcgc gtcgaagagc
      301 ccgtcgcgca cgaactcgac gagatcgcgg acctcctggt cgtcgagccg gaccgccttg
      361 cgcagccgcg gatcgatgcc ggcgtggagg atgcccatga acggcccgac gctcagatcg
      421 gccggcagcg cgttggccgc cgggtcgtac tggcgcaggg agcgatcgac gtcgaggtgg
      481 tgcgcgatcg ccgcctcgag gctcaggaac gcgccgttat ggaagaaccc gggggagacc
      541 ttcaggttcc ggaggggcgc ggtgcggaac atgtagcgca gggccgggtc cccggtggtc
      601 tgctcgaacc cgaagtcctc gttctcgtcc ggaccatcga agatgacgtt gccggtcccg
      661 acgccgaaga ccggcgccac ctggggcccg ccgatgcggt gcggctggaa gtcgctgaac
      721 atctcgttcg caggcccggc caccgcgtgg caggagacgc accccgcctc gccgaagaac
      781 agcagggcgc cgcgcttctg cccgatcgtc atcgcgccgg gctcgccgcg cgcgaagcga
      841 tcgagcgggg cgttcgcggc cgtgaggttc gcctggaact cggcgatcgc gaggcggcgc
      901 atgtcgatcg tgatcgcgcc cgcggcgagc ggcaccccgc cgttgaagac ctcgccgaat
      961 ttctgcaagt agagggcatt ggcgttgagc cgcgcgtcca ccgccgcctg gatcgggaag
     1021 ttgtgcacgc cggtgccgtc gggcgccggg aggacctgcc cctgtccgtc gtcgaactgg
     1081 aagtgccgcg gcccgagcgg gccggggttg gcggtgatgc cgttgaagcc cgccatctcg
     1141 accagctcgg tcgaggggag cgatccctgc gccgacagga gcgtcggcac gccggtgacg
     1201 acgttctcgg gcggcgggaa ctggaacccg agcgacgcgt cgaacgggtc gcccgacagc
     1261 gccacgaagc ggggcgtcca catcagggcc gggtagaaga cggtgttcgc caccagcggt
     1321 gagcgcctct ggttgcgcgg gccgtggcgg ctcggcccga ccacgtcgtt gttctgcacg
     1381 ccgatcgcca tcctctggga atcgccgaac ccgaacgcgg gcgcgtggca cccggcgcag
     1441 gagttgtcgt catgcaggcc gaggatcttg tcgaagaaga gaaggcgccc gagatcggcg
     1501 cgcgccgggt cgatggggcg gccgagccgg gccgcgagcg tcgactcgac ccggccggtg
     1561 aatccctccc gggccaggac cagggccagg cgctggtcga tgttggcctg tcccgggttg
     1621 ccgccgggcg gtgcggatcg ccccgtggcc gccgcgagcc cgtaggctgc gagaaaggct
     1681 cccgccgcga cgaccagcgc cggcgccgca cggcggagac tgcacgaaat gcgcgcgagg
     1741 acgatcatga cgcacctcgt tctgcgaagg ggggacgccc cctcgcctcc cgacggcgcg
     1801 ggcgcgtccc ggattaccgg atgagttcct gggttgttgg cccggaatat acgacctccc
     1861 ggacgcgcgg ggcaatacct ttctgccgcc cgcgcccggt ctcgcgtcgc gccgcggcgg
     1921 cgcgcgcagg ccgcgatcgg tcgttacccg ggacgagtcg gcgccgtagt ccggtcatgc
     1981 ttctgccctg agagcgcccc gccgagcagg ctcatcgtgt cgatcggctg cctctcaatt
     2041 gggtccggct gcgttgccga caacacgacg cagaccgcgt tggtcggggt ccatcagttg
     2101 agcgccgggt tgtacctcga gggtgtcgtc cgcgcgtacg tggagtagcc gctcagtcgc
     2161 cgcgcgctcc gggcgggtgg gtcagaccgt cgcgcacgag aaggtcgggg tcctctttca
     2221 accgctcgag aatcgcggag gtgccgagcg cggcgtcgtc gcgcaggacc gtggcggcat
     2281 agcggcgcac ccaccaggcg ggatcggcgc tcagcgtcgc gagcgccgcc gcggcgcggg
     2341 cgcgctcctc ctccttcagg gggcgtgaga catcgcggct cgttacgatg ctcttcagct
     2401 cgtcgcgcga ccgcatcgac ccgggcggcg gcggtgccgc ccggccgtag acacgctgca
     2461 tgagaaggac ggcctcgtcc ggcgacacat cgtagagata cgcgacgaag gcggccggcg
     2521 gacggccgcc gcgccccgcg agccacgcgt cgtagacgcg gaaatccgct tcgcccccgt
     2581 cgcggcgatc gacagtggcg agcatcccgg caaggacgcg gcgcagcgcc ggggcggcat
     2641 cctcgaggct cggccccacc gcttcgatcg tctctttcgg cgtgaaccca atccggcgca
     2701 ggacgatcgc cccggccatg ccgcgatcgg tgccttcggc gccttcgaga tacagcgcca
     2761 tctgaagcag gagcgctgtc cggtcgcgtt cccccagccg ctgcagcgcc gcgaagcgat
     2821 cggcggccgc ctcgtcgttc cgcacgatgg ccgacaccgt ctcctcgatg gcggcctgcg
     2881 cgcgcggatc gggatcgagg cgaccggaga ccggctcgcc gcacatcacc gcgccggcgg
     2941 cggcgcccat catccaggct gcgagcgcga gccgtccgat ccccgccagg aacccgatcg
     3001 cccggcgcgg aggggacatc cgcctagtag gggacatgga tcaccatgag atccagcagg
     3061 ccctggagca cttcagccgc gtcgtcgatc gtgaaatggg tgagcccgga gccccacggg
     3121 gtcgcggtca cgttgacatt gagattggcc ccgggaaccg gcccgccgca gagctgcagg
     3181 aagccgcagc cggggtgttc gtaatagttg gcgtggtacg ccgtcgtggg gggcagcgcc
     3241 gtctcggggt cgatgt
//
LOCUS       DASRIQ010000529        10725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_980969, whole genome shotgun sequence.
ACCESSION   DASRIQ010000529 DASRIQ010000000
VERSION     DASRIQ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980969"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..907)
                     /locus_tag="VFD06_12960"
     CDS             complement(104..907)
                     /locus_tag="VFD06_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN04485.1"
                     /translation="MRSHVALVLDATLGEPLFAKHEDLVAPIASITKLMTAMVILDSG
                     LSPDEPLTIDRADVDTVKLTGSRLPVGASIRRVDLMTIALMSSDNRAAAALARSYPGG
                     TAACLDAMNRRAIALGMTRTHFADATGLSSLNVSTAGDLTRLVLAAARYPAIREATTS
                     RLHRLTLGNGRVLEFRNSNGLVSSPAWSIGLSKTGYINEAGRCLVMQAEIAARQVVIV
                     LLDSWGKYTRLGDANRIRHWLEGSVPHAPAAAGTKVAGDAPSPVRAPAP"
     gene            1220..4876
                     /locus_tag="VFD06_12965"
     CDS             1220..4876
                     /locus_tag="VFD06_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04486.1"
                     /translation="MPSSRTIPLLLSILAGLLFAVPAHSKPVRETAAAPGAPLQKGKP
                     GKKGEQEKEKKRKGEARPGAPSGVRRGGPDAGDEPGAAATIRAHVNARGQTVFSIAPV
                     RFDVSPPLRELATREMPHLEEEEESPTNPLLPAWRIPRSGLPDPVVQSEIGFGASLRQ
                     PIALQVPTTGFDFLGVVQSGAVPPDTNGAVGNDQYVETVNTRYQVWSLDRGTKAATPL
                     LGPALINTLWAGFGGACQTQNAGDPIVLFDRLANRWLISQFTTGISGGFYFQCVALST
                     TADATGTYARWAFAVPNGIFGDYPHFGVWTDAYYMMAHGFTSSGAGGSYLAGIFAAMD
                     RPKMLAGDPTATWQVILDPDEGGHMPADQDGPTPPPTRAPGIFLSLHGPSMYFYRMKV
                     DFAVPANTTRILQGTAPVAPATGACILFADPGTCIPQPGTTTQLDSLGDRLMFRAAYR
                     KFVDHESIVISHSVDPFVTGLVAGVRWYDFRLSGGPDPACPSYPCTWQQGTIADVVNG
                     RNRWMPSIAMDGAENILVGYSVSGKTHLAENHSIRYTGRAKNDPPGLMTVPEVTVATG
                     VRNNINNSRWGDYTSMSVDPFDDCTFWHANEYYFTANNSWATRIASAAWPAGTGDGQC
                     QPISCSSRPASAPLIGAAIVPGQNQIQVSWNAIAPPAGSYAIERAVGACGSEGIYQPL
                     GFVGGASTIFTDTTVQGGITYSYRVIAAADGAGKCQALVASGCASTTATGTCNLKPDF
                     DGAADATSAAASICGINLSWGAGSSSCPLTPNVRYNVYRGTVPDFVPSAANRVAACAD
                     GPDSYLDQANLASGVTYYYVVRAEDDSTGNGGPCGGGNEEANGIVVNGTAYGAGLQPA
                     PGTWADNGGDGTSLLRRNAPGEGNSPEPAWRFVKTANDPGANHTTGGAYAYRNAGPAA
                     GDAYLPLICAEMRTPPIPVGASSVNLTYWERHQLEYHWDGVVVEFSLNDGAWTTVPAP
                     SNSILAGCSATAAVTNWETLSCTGAPPANSCGYPATQSAINGPLGGGVDCNTWSTSGA
                     VTAYANRCHPLTGLSPGDTIQFRWRFTSDPAAEFAGFYLNDVEITNVLLPNACVPGVG
                     CGGQPDGTACDDANPCTAGTTCTSGACGGGAPVQPPEINDSVAFGADGETLSWDDPPG
                     AYNVYRGLRTSPWTYDQTCLQPHVAGSSVSDAAEPAPGQIFFYLVTRLDACGESTPGQ
                     DSSGTPRPNPAPCP"
     gene            complement(4907..5137)
                     /locus_tag="VFD06_12970"
     CDS             complement(4907..5137)
                     /locus_tag="VFD06_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04487.1"
                     /translation="MNRLRLIALALIVAGVLGLVYSRFSYTKETHGFQVGSLEVAVKD
                     RETVNIPLWVSVGALVAGTALLLTRRSGSAPA"
     gene            complement(5140..5814)
                     /locus_tag="VFD06_12975"
     CDS             complement(5140..5814)
                     /locus_tag="VFD06_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZN04488.1"
                     /translation="MLRASRGAAGILCLVFLAQSGCASTQSNTTTSSERATKRDKTAK
                     GAGIGAAAGAVIAAVTGERTADRILAGAAIGAGIGAGVGVYMDKQEEKLGHIPNTTVE
                     RVSDDTLLVHFESDVLFDVDSATVKADARDALDEASQVFTEFPKTAIVVQGHTDSTGS
                     ETHNQDLSERRAHAVVAYLTGKGIDPERMAPMGYGEGQPIASNETTGGRAQNRRVDLL
                     LKAKAK"
     gene            complement(5964..6116)
                     /locus_tag="VFD06_12980"
     CDS             complement(5964..6116)
                     /locus_tag="VFD06_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04489.1"
                     /translation="MIEKLRSGKYRLYSRKKNPKTGRRRNLGTFRTRAAAAKHEREVQ
                     GFKRRG"
     gene            complement(6113..8725)
                     /gene="ligD"
                     /locus_tag="VFD06_12985"
     CDS             complement(6113..8725)
                     /gene="ligD"
                     /locus_tag="VFD06_12985"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HZN04490.1"
                     /translation="MALRTYRRKRRFDRSPEPRGRPRARRRRGGLAYVVQKHDATRLH
                     YDFRLELDGVMKSWAIPKGPSLDPGVKRLAVHVEDHPIEYNRFEGVIPKGEYGGGPVI
                     LWDRGTWSPEEDAREGYRRGTLKFRLEGEKLRGSWVLVRTRQTQGSKEQWLLIKHDDA
                     EARRGAKTDIVNERPESVVSGRAVEEIGPSFHRRSSAARRTRPTARRTAPAAGAPRPD
                     ALPGARRAALPAALEPELATLVESAPAGEDWLHEIKFDGYRALCRIDRGKARLFTRRG
                     NDWTAAFAPIARAAAALPVRSAWIDGEVVVVGKNGVSSFGGLQEALGGERGGSDLLYF
                     VFDLPYLDGFDLRSVPLLERKTILASLMRTSGRDGRGPIRYSDHILGKGEAFFEQACR
                     SGLEGTIAKRAKSLYRAGRGRDWIKVKCLQTREFAIVGFTDPSGARRGFGALVLGQAD
                     DGRLVHVGRVGTGFSDRMLRDLAPRLRALEVAKPPVDPAPRGAAARRVHWIRPQLVAD
                     VAFTERTRDGILRHPTFRGLRADKTPAETAGEAAGTALQPRRAARSRGTNRGRRGRTP
                     GTGGALRLTNPEKALWPDAGITKRDLVDYYLQVQERLIPYVKDRPLTVYRCPDGLGKF
                     CFWAKHLEDPLPPGLRPISLDDAKTEKTRGAYFYAASMEGVLALAQLGALELHVWGSR
                     VAKVEYPDLMIFDIDPDVGLPWTRVLEGCATLRARLDDLGLRSWPKTTGGKGLHVCVP
                     LGRRQTWDEVKAFSRALAEDIARGEPAKYTVNLLKARRKGRIFIDYLRNGRGATAVAA
                     WSTRARPGAPVSVPLAWDELDAALRPSSFTVRTAGERLKRRDPWSGFAACRQTITAAM
                     RKAVGLEDARRSAR"
     gene            complement(8748..9671)
                     /locus_tag="VFD06_12990"
     CDS             complement(8748..9671)
                     /locus_tag="VFD06_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZN04491.1"
                     /translation="MARAIWKGSISFGLVSIPVALHGAENSEELSFHQLDRRDLNPVG
                     YKRFNKKTDEEVPWDQVVRGYQYGRGRYVVVTDQDIKRANVEATQTIDIVGFVEGSQI
                     PPLYYERPYYLEPQKGGAKAYALLRETLRQTGMVGVARVVIRSRQHMAALLERQGVLV
                     LEILRWAHELREPKGLEVPDDSRARVPKKETDMAVRLVKGMAAKWEPKKYKDTFNDDL
                     MALIRRRISSGKTTEIDESEPKAPPKRGGKVVDLMPLLRKSLEERGGEARSRSRARAH
                     AAPGSAAASARGRSAGAGNGRGHGAGRRHAR"
     gene            9858..10076
                     /locus_tag="VFD06_12995"
     CDS             9858..10076
                     /locus_tag="VFD06_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HZN04492.1"
                     /translation="MNRNASRRGRRRYGPAASRSVESAMRRRKRGTLKSGRGGRGGRV
                     KSHKQAIAIGLSEARKKGAKVPPPPDAS"
     gene            10147..>10725
                     /locus_tag="VFD06_13000"
     CDS             10147..>10725
                     /locus_tag="VFD06_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04493.1"
                     /translation="MRMNLLHRVAAVVFSASLLAVPAGAAAKEKLDGYLEYKKPPYVI
                     VDGQRVEIDRTTKLDAGRIRNVFDIPLGWHVKVRGTRAKDGTIVAGDLEAGKNGSEFM
                     ESDVLAATDQAEKKYVETKKVSDTGRDGKEHVVGRLIDTGPEVERARRIVDRLLPPYV
                     SKEQVRVYVVDNEAWNAMAMANYSIYVFRGLMK"
BASE COUNT         1577 a         3841 c         3708 g         1599 t
ORIGIN      
        1 agcgggtcga tggagtcctg gatgactgcg ggcagctgca tcggtttgtt cggtgctccg
       61 ccgcgacgat agcagcggcc cgacgccggg acagttggga gaactacgga gccggggcgc
      121 gcaccgggga cggcgcgtct ccggcgacct tcgtgccggc ggccgccgga gcgtggggga
      181 cggacccttc gagccagtgg cggatgcggt tggcgtcccc cagccgggtg tatttccccc
      241 acgagtcgag gagcacgatg accacctggc gcgccgcgat ctccgcctgc atcacgaggc
      301 agcggccggc ttcgttgatg tagccggtct tggagaggcc gatgctccag gccgggctgc
      361 tcacgagccc gttggagttg cggaactcca gcacccggcc gttgccgagc gtcagccggt
      421 ggagcctgga cgtcgtcgcc tcgcgaatcg ccggatagcg cgccgccgcc aggacgagcc
      481 gagtcagatc gccggccgtc gagacgttga ggctcgacag gccggtcgca tcggcgaaat
      541 gggtccgcgt catgccgagc gcgattgccc ggcggttcat cgcgtcgagg cacgcggcgg
      601 tcccgccggg ataggagcgc gccagggcgg ccgcggcgcg gttgtcggac gacatgaggg
      661 cgatcgtcat cagatcgacg cgacggatgg aggctcccac cggaagtcgc gagccggtga
      721 gcttgacggt gtcgacgtcg gcgcggtcga tcgtcagcgg ttcatcgggg gagaggcccg
      781 agtccaggat caccatggcg gtcatcagct tggtgatcga agcgatcggg gccaccaggt
      841 cctcgtgctt cgcgaacagc ggctcgccga gggtcgcgtc cagcaccaga gcgacgtgcg
      901 atcgcacctg agggtccaac cccctgctcg gacggggggt gtggggcggc gcagcaggag
      961 gattcgcggc gatcgggaga cattcgggtg ccagcagggc cacggccacc atggccggca
     1021 tccaccggag aacgaatcga gccagaaaca cccccaccgc ccgcccgggc gtgaggcgca
     1081 acgtatcagg aatcaccctg cgggaaaagc cgaattcgcg gttttttgat tgacggatcg
     1141 gtccgatcgc acgacaattc tgctgcaatc cgctccgcac caacctcagc gggagaccgg
     1201 ccgaatgccc ccgtccagga tgccgtcgtc gcgaaccatt ccgctcctcc tgtcgatcct
     1261 cgccggcctg ctcttcgccg tgcccgcaca ctcgaaaccg gtccgggaga cagcggccgc
     1321 accgggcgct ccgttgcaga aggggaagcc gggaaagaag ggggagcagg agaaggagaa
     1381 gaagaggaaa ggggaggcca ggcccggcgc gccgtccggg gtgcgccgcg gcggcccgga
     1441 tgccggcgac gagccggggg cggcggcgac gatccgggcc cacgtcaacg cccgcggcca
     1501 gaccgtcttc tcgatcgcgc ccgtccggtt cgacgtctcg cccccgctgc gggagctcgc
     1561 gaccagggag atgccgcatc ttgaggagga ggaagaatcc ccgacgaacc cgctcctccc
     1621 ggcgtggcgc attccgcgtt cggggctgcc ggacccggtc gtgcagtcgg agatcggctt
     1681 cggcgcctcc ttgaggcagc cgatcgcgct ccaggtgccg acgaccgggt tcgatttcct
     1741 cggggtcgtc cagtcgggcg cggttccccc cgacacgaac ggcgcggtgg ggaacgacca
     1801 gtacgtcgag acggtcaaca cccggtacca ggtctggtcg ctcgaccgcg gcacgaaagc
     1861 cgccaccccg ctcctcggcc ccgcgctcat caacacgctc tgggccgggt tcggcggcgc
     1921 ctgccagacg cagaacgccg gcgatccgat cgtgctgttc gaccggctgg ccaaccgctg
     1981 gctgatctcg cagttcacga ccggcatcag cggcggcttc tacttccagt gcgtcgccct
     2041 ctcgaccacc gccgacgcga ccggcaccta cgcccgctgg gccttcgcgg ttccgaacgg
     2101 gatcttcggc gactacccgc acttcggcgt ctggaccgac gcctactaca tgatggcgca
     2161 cgggttcacg tcctcgggcg cggggggcag ctatctcgcc gggatcttcg ccgccatgga
     2221 tcggcccaag atgctggccg gtgatccgac cgcgacctgg caggtgatcc tcgacccgga
     2281 cgaggggggg cacatgccgg ccgatcagga tgggccgacc ccgccgccga cccgggcgcc
     2341 ggggatcttc ctgtcgctgc acggtccgtc gatgtacttc taccggatga aggtggactt
     2401 cgccgtgccg gcgaacacga cgcggatcct ccaggggacg gcaccggtcg ccccggccac
     2461 cggcgcgtgc atcctgttcg ccgacccggg cacctgcatt ccgcagcctg ggaccacgac
     2521 ccagctcgat tcgctcggcg atcgcctgat gttccgggcc gcctaccgca aattcgtcga
     2581 ccacgagtcg atcgtcatca gccactcggt cgatcccttc gtcacggggc tggtcgccgg
     2641 cgtgcgctgg tacgacttcc gcctgtcggg ggggccggat ccggcctgcc cgtcctatcc
     2701 gtgcacctgg cagcagggga ccatcgccga cgtcgtcaac gggcgcaacc gctggatgcc
     2761 gtcgatcgcc atggacggcg ccgagaacat cctcgtcggc tacagcgtct cggggaagac
     2821 ccacctggcg gagaaccaca gcatccgcta caccggccgg gcgaagaacg atcccccggg
     2881 cctgatgacc gtccccgagg tgaccgtcgc caccggcgtc cggaacaaca tcaacaacag
     2941 ccgctggggc gattacacca gcatgagcgt cgatccgttc gacgactgca cgttctggca
     3001 cgcgaacgag tactacttca ccgccaacaa cagctgggcc acgcgcatcg cgtcggccgc
     3061 ctggcccgcg gggaccggcg acggccagtg ccagccgatc tcgtgctcct cgcgtccggc
     3121 gtccgccccc ctcatcggcg cggccatcgt cccgggacag aaccagatcc aggtgagctg
     3181 gaacgcgatc gcgccgccgg ccggctcgta cgccatcgag cgcgccgtcg gggcctgcgg
     3241 cagcgaaggg atctaccagc cgctgggatt cgtcggcggc gcctccacga tcttcaccga
     3301 caccacggtg cagggaggga tcacctattc ctaccgggtg atcgccgccg ccgacggggc
     3361 ggggaagtgc caggcgctcg tggccagtgg ctgcgccagc accacggcca ccgggacctg
     3421 caatctcaag ccggacttcg acggggccgc ggacgcgacg agcgccgccg cctcgatctg
     3481 cggcatcaac ctgtcgtggg gcgcgggctc ctccagctgc cccctgacgc cgaacgtccg
     3541 ctacaacgtg taccgcggca ccgttcccga cttcgtcccg tccgccgcca accgggtcgc
     3601 cgcctgcgcc gacggccccg actcgtacct cgatcaggcc aacctggcga gcggcgtcac
     3661 ctattactac gtggtgcggg ccgaggacga cagcaccggc aacggcggcc cctgcggcgg
     3721 cggcaacgag gaggcgaacg gcatcgtggt caacggcacc gcgtacggcg ccggcctgca
     3781 gcccgccccg gggacctggg ccgacaacgg cggcgacggc acgtcgctcc tgcgccggaa
     3841 cgcgccgggg gagggcaatt cccccgagcc ggcctggcgt ttcgtgaaga cggcgaacga
     3901 cccgggcgcc aaccacacca cgggcggcgc gtacgcctac cgcaacgccg gcccggcggc
     3961 cggagacgcc tatctgccct tgatctgcgc ggagatgcgg acgccgccga tccccgtcgg
     4021 cgcatcctcg gtgaatctga cgtactggga gcggcaccag ctcgagtacc actgggacgg
     4081 cgtcgtcgtg gagttctcgc tgaacgatgg agcgtggacg accgtccccg ccccgagcaa
     4141 ctcgatcctg gccggctgct cggccaccgc cgcggtgacg aactgggaga cgctgtcgtg
     4201 caccggcgcc ccgcccgcca actcctgcgg ctacccggcg acgcagagcg ccatcaacgg
     4261 cccgctcggc ggcggggtcg actgcaacac ctggtcgacg agcggcgcgg tcaccgccta
     4321 cgcgaaccgc tgccatcctc tgaccggcct cagccccggg gacacgatcc agttccgctg
     4381 gcgcttcacg tcggaccctg cggccgagtt cgccggcttc tacctcaacg acgtggagat
     4441 caccaacgtc ctgctgccga acgcctgcgt tccgggcgtc ggctgcggcg gacagccgga
     4501 cggcacggcg tgcgacgacg ccaatccgtg caccgccggg acgacctgca cctccggggc
     4561 gtgcggtggt ggagcgccgg tgcaaccgcc ggagatcaac gacagcgtgg cgttcggcgc
     4621 cgacggcgag accctctcct gggacgatcc gcccggcgcc tacaacgtct accgcgggct
     4681 caggacgagc ccctggacct acgaccagac ctgcctgcag ccgcacgtgg cgggctcgag
     4741 cgtcagcgac gcggcagagc cggcgccggg tcagatcttc ttctacctgg tgacgcgcct
     4801 ggacgcctgc ggtgaatcga cgcccgggca ggactcgtcg gggacgccgc gaccgaaccc
     4861 ggccccctgc ccctgacggc gcggcggccg gatgccgtcc cccgtctcag gcgggggcgg
     4921 agccggagcg gcgggtgagg aggagcgcgg tcccggccac cagggcgccg acgctgaccc
     4981 agagcgggat gttgaccgtc tcccggtcct tcacggccac ctcgagcgag ccgacctgga
     5041 acccgtgcgt ctccttcgta taggagaacc ggctgtacac caggccgaga actcccgcga
     5101 cgatcagcgc cagggcgatg aggcgaaggc ggttcatcgt cacttcgcct tcgccttgag
     5161 gagcagatcg acccggcggt tctgcgcccg gccgcccgtc gtctcgttcg aggcgatcgg
     5221 ctgaccctcg ccgtagccca tcggcgccat gcgctccggg tcgatcccct tgccggtgag
     5281 ataggcgacg accgcgtggg cgcgccgctc tgagagatcc tggttgtgcg tttcgcttcc
     5341 ggtcgagtcg gtgtgcccct ggaccacgat ggcggtcttg gggaactcgg tgaagacctg
     5401 cgacgcctcg tccagcgcgt cgcgcgcgtc cgccttgacg gtggcggagt cgacgtcgaa
     5461 cagcacgtcc gactcgaagt gcacgaggag cgtgtcgtca ctcacccgtt ccaccgtggt
     5521 gttcgggatg tgtccgagct tctcctcctg cttgtccatg tagacgccga ccccggcgcc
     5581 gatgccggcg ccgatcgccg ccccggcgag gatgcgatcc gccgtgcgct cgccggtcac
     5641 cgccgcgatc accgctccgg cggcggcgcc gatgccggcc cccttggcgg tcttgtcccg
     5701 cttcgtcgcc cgctcgctcg acgtcgtcgt gttcgactgg gtcgacgcgc agccggactg
     5761 cgcgaggaac acgagacaca gaattccggc cgctccgcga ctcgctctaa gcatcgtcat
     5821 ccggtccctc ctccgatggc tgttccgttt gatgagcgcg atgcaagcgc gacaccatgg
     5881 tgacgccgac gtccccgcgc gggaagggca ctccgatttt tccgacgccc cgatcaggga
     5941 aaaccccgcc ttcgccaagg cgcctagccg cggcgcttga acccctgcac ctcgcgctcg
     6001 tgcttcgcgg ccgcggcgcg ggtgcggaac gtcccgaggt tgcggcgccg gccggtcttc
     6061 gggttcttct tgcgcgaata aagacggtat ttccccgaac gcagcttctc gatcatcgcg
     6121 cgctcctgcg cgcgtcctcg aggccgacgg ccttccgcat cgccgccgtg atcgtctgcc
     6181 ggcaggcggc gaagccgctc cacggatccc ggcgcttcag gcgctcccct gccgtcctca
     6241 ccgtgaagga ggagggacgg agcgccgcgt ccagctcgtc ccacgcgagc gggaccgaga
     6301 ccggcgcgcc gggacgggcg cgcgtcgacc aggcggcgac ggcggtcgcg ccgcgtccgt
     6361 tgcgcagata gtcgatgaag atccggccct tgcgccgcgc cttgagcagg ttgacggtgt
     6421 acttcgccgg ctcgccgcgg gcgatgtcct ccgcgagggc gcggctgaac gccttcacct
     6481 cgtcccaggt ctgccggcgc ccgagcggca cgcagacgtg cagccccttc cctcccgtcg
     6541 tcttcggcca cgatcgcagc ccgaggtcgt cgaggcgggc gcgcagcgtc gcgcatccct
     6601 cgaggacccg cgtccagggg aggccgacgt ccgggtcgat gtcgaagatc atgagatcgg
     6661 gatactccac tttcgcgacg cgcgaccccc agacgtgcag ctccagggcg ccgagctgcg
     6721 ccagcgccag caccccttcc atcgacgcgg cgtagaagta cgcgccgcgc gtcttctcgg
     6781 tcttcgcgtc gtcgagcgag atggggcgga gccccggggg gagcgggtct tcgaggtgct
     6841 tcgcccagaa gcagaatttc ccgaggccgt cgggacaccg gtacacggtc agcgggcggt
     6901 ccttcacgta cggtatcagg cgctcctgca cctggaggta gtagtcgacg agatcgcgct
     6961 tggtgatccc ggcgtccggc cagagcgcct tctccgggtt ggtgaggcgg agcgctcctc
     7021 ccgttcccgg ggtcctccca cgacgccccc gattcgtccc gcgcgaccgg gcggccctcc
     7081 gcggctgcag cgcggtgccg gccgcctcgc ccgcggtctc cgccggcgtc ttgtcggcgc
     7141 gcaggccgcg gaaggtcgga tgacgcagga tgccgtcccg cgtccgctcg gtgaacgcga
     7201 cgtcggcgac gagctgcggc cggatccagt ggacgcggcg cgccgcggcg ccgcgcgggg
     7261 ccggatcgac cggaggcttc gccacctcga gcgcccgcag ccgcggcgcc agatcccgga
     7321 gcatgcgatc gctgaagccg gtcccgaccc gcccgacgtg cacgaggcgg ccgtcgtcgg
     7381 cctgtcccag gacgagcgct ccgaatcccc ggcgcgcgcc ggacgggtcg gtgaagccga
     7441 cgatcgcgaa ttcgcgcgtc tgcaggcact tcactttgat ccagtccctc ccgcgccccg
     7501 cccggtagag gctcttcgcg cgcttcgcga tcgtcccctc cagtcccgag cggcaggcct
     7561 gctcgaagaa ggcctccccc ttcccgagga tgtgatcgga atagcggatc ggcccgcggc
     7621 cgtcgcgccc ggacgttcgc atcaggctcg cgaggatcgt cttgcgctcg aggagcggga
     7681 cggagcggag gtcgaatccg tcgaggtagg ggagatcgaa gacgaagtac aggaggtccg
     7741 agccgccgcg ctcgccgccc aatgcttcct gaagcccgcc gaagctgctg acgccgttct
     7801 ttcccacgac caccacctcg ccgtcgatcc aggcggagcg gaccggcagc gccgcggcgg
     7861 cccgggcgat cggggcgaag gccgcggtcc agtcgttccc ccggcgcgtg aagaggcgcg
     7921 ccttgccgcg atcgatccgg cacagggcac ggtagccgtc gaacttgatc tcgtgcagcc
     7981 agtcctcgcc ggcgggggcc gactcgacga gcgtggccag ctccggttcg agcgcggccg
     8041 gcagcgccgc ccgccgcgcc ccggggaggg cgtccgggcg cggcgcgccg gcggcgggcg
     8101 cggtccgacg ggcggtgggt ctggtccggc gggcggcgga cgatcgcctg tggaatgagg
     8161 ggccgatctc ctcgacggcg cggccgctga cgacgctctc cggacgctcg ttcacgatgt
     8221 ctgtcttcgc gccgcggcgg gcctccgcgt cgtcatgctt gatcaacagc cactgctcct
     8281 tgcttccctg cgtctgccgc gtgcggacca agacccacga gccgcggagc ttctccccct
     8341 cgagccggaa cttgagcgtt cccctgcggt acccctcgcg cgcgtcctcc tcgggactcc
     8401 aggtgccgcg atcccagagg atcaccggcc cgccgccgta ctctcccttc gggatcacgc
     8461 cttcgaagcg gttgtactcg atcgggtgat cctcgacgtg caccgccagg cgcttcacgc
     8521 ccgggtcgag gctgggccct ttcgggatcg cccacgactt catgacgccg tcgagctcga
     8581 ggcggaagtc gtagtgcagc cgcgtcgcgt cgtgcttctg cacgacgtag gcgaggcctc
     8641 cccggcggcg ccgcgcgcgc ggccggccgc ggggctcggg ggaccggtcg aagcgacgct
     8701 tgcggcgata ggtgcggagc gccatcggcc tagaccggct cctcacctca gcgcgcatgg
     8761 cgacggccgg cgccatgacc ccggccgttg ccggcgcccg cgctccggcc gcgtgcggag
     8821 gcggcggcgc tccccggtgc ggcgtgggcg cgcgcgcgtg accggctccg cgcttctcct
     8881 ccccgctcct ccaggctctt cctgagaagc ggcatcaggt cgacgacctt gcctcctcgc
     8941 ttcggcgggg ccttcggctc gctctcgtcg atctcggtcg tcttcccgga ggagattctt
     9001 ctgcggatga gcgccatgag gtcgtcgttg aaggtgtcct tgtacttctt cggttcccac
     9061 ttcgcggcca tccccttcac cagtcgcacc gccatgtcgg tctccttttt ggggacccgc
     9121 gccctggagt cgtcggggac ctcgagcccc ttcggctcgc gcagctcgtg cgcccagcgg
     9181 aggatctcca ggaccaggac gccctgccgc tccaggagcg ccgccatgtg ctgccgcgag
     9241 cggatgacga cgcgggcgac tccgaccatg ccagtctggc gcagcgtctc gcgcaggagg
     9301 gcgtacgcct tcgctccgcc cttctgcggc tcgagatagt aggggcgctc gtagtaaagg
     9361 ggcgggatct gcgagccctc gacgaagccg acgatgtcga tcgtctgcgt cgcctcgacg
     9421 ttggcacgct tgatgtcctg atcggtcacc acgacgtagc ggccgcgccc gtactggtag
     9481 ccccggacca cctggtccca ggggacttcc tcgtcggtct tcttgttgaa acgtttgtac
     9541 ccgacagggt tcagatcgcg ccgatcgagc tggtggaacg acagctcctc gctgttttcg
     9601 gcgccgtgaa gcgccaccgg gatgctcacc agaccgaaac tgatgctgcc tttccagatt
     9661 gcgcgcgcca tggacgcacc tcatgtcttt cccgatgaga ttacgctcgc gccgccgcac
     9721 cggcaacgcg ccgcgttgca ccgacgcgcg ccggaacgcg gacacgcgcc gcgatctggc
     9781 ccgggatttg cgaactgtgt tcgagcgagg gtgcgcgaag catccgtagt tcaaggagga
     9841 acgctatgag gaatcgtatg aaccgcaacg ccagtcgccg cggccggcgt cgttacggtc
     9901 cggccgcgag ccgcagcgtg gagagcgcca tgcggcgccg caagcgcggc acgctgaaga
     9961 gcggccgggg cggccgcggc gggcgggtga agagtcacaa gcaggcgatc gccatcggtc
    10021 tgtcggaagc ccggaagaag ggggcgaagg tccctccgcc ccccgacgcg tcctgacgcg
    10081 cgccacccgg gaccgcgtgg tcccgcgggt cccgtgtccg atcttcgttt ccggaggtga
    10141 ggtccgatga ggatgaacct gttgcaccgg gtggcggcgg tggtcttcag cgcgtcgctc
    10201 ctggccgttc cggccggggc cgcggcgaag gaaaagctcg acggctatct cgagtacaag
    10261 aagcctccgt acgtcatcgt cgacggccag cgcgtcgaga tcgacaggac gacgaagctc
    10321 gacgccggtc ggatcaggaa cgtcttcgac atcccgctcg gctggcacgt caaggtcaga
    10381 gggacccgtg ccaaggacgg caccatcgtg gcgggcgacc tggaggccgg gaagaacggc
    10441 tccgagttca tggagagcga tgtcctcgcc gccaccgacc aggcggagaa gaagtacgtg
    10501 gaaacgaaga aggtgtcgga cacgggacgc gacgggaagg agcacgtcgt cgggcggctc
    10561 atcgacaccg gccccgaggt ggagcgggcc cgacgcatcg tcgaccggct gctgccgccg
    10621 tacgtctcga aggagcaggt gcgcgtctac gtcgtggaca acgaggcgtg gaacgccatg
    10681 gcgatggcca actactccat ttacgtcttc cgcgggttga tgaag
//
LOCUS       DASRIQ010000530         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2541053, whole genome shotgun sequence.
ACCESSION   DASRIQ010000530 DASRIQ010000000
VERSION     DASRIQ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541053"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFD06_13005"
     CDS             complement(<1..797)
                     /locus_tag="VFD06_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZN04494.1"
                     /translation="MPTTPEPVRPVTLPEIREARERIAKTVVRTPLVRLELGPDAPDI
                     RLKLENLQPINAYKLRGAANAVASLTDAERRKGVWTISAGNAGQGVAYAARAAGVPCT
                     VVAIETAPQAKLDRMKALGAKLILVSYTTAWKALDDRAYPGAEGTFVHPFDDHQFIAG
                     HATMGLEILEDAPDVTAVIAAIGGGGLITGVASAIKALKPEVRIFGAEPETAAPMSRS
                     LAAGSPQTFPEWKASFVDGAGGQSVFPRMWERMKPLVDDSIVVTLDQT"
     gene            complement(854..3598)
                     /locus_tag="VFD06_13010"
     CDS             complement(854..3598)
                     /locus_tag="VFD06_13010"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN04495.1"
                     /translation="MLRCGACRGESPDGGRFCLHCAAPLAPGTAGPTRTVRDDPPARP
                     IAPHPSPFRSGGTPEGRFLPGTMLAGRYRIFGLVGRGGMGEVYRAEDLKLGQTVALKF
                     LPREVERDEGRLARFLEEVRLARQISHPNVCRVYDVGEADGQHFLSMEFVDGEDLAGL
                     LRRIDRLPRDKAIQIARQLCAGLQAAHEQGILHRDLKPSNVMIDGRGRTRITDFGLAR
                     LAGEIQGADLRAGTPAFMAPEQIAGTAVTVKSDLYALGMVLYELCTGHQAFKAASPAE
                     SARLKMESAPTSPSALIEGFDPAVERIILRCLERDPARRPATALAVAAALPGGDPLAA
                     ALAAGETPSPELVAEAGTTEALKPALAWAGLAAFLLLLPAAVFVSGRSQLARLVPLPK
                     SPEILRERAREIVRDLGYVEPPIDSVSGFDPENEYLDHLAAGQAARPGWDTLRAGPPY
                     GIAFWYRQSPRPMKPWGDAGATPSTFSDPPLIDSGMVRLRLDPEGHLRTFQAVPPDRD
                     PQAAPPLEPDWNRLLALGGHEPAAFKAVEPSWVPRMYADRRAAWEGTDPRMPGMTLRL
                     EAASYLGRPIFFRTVEPWTRARLSEPATAALPGSVLQEWASMVWIVVAISIMSAGVIV
                     ARRNLRLGRGDRKGAFRLAAFMLLSAEIAWLLETHHVASNAFANFTINLAVRLLIAGL
                     VWVFYLALEPYLRRNWPQVLVSWVRLLDGRLRDPLIGRDLLFGAVLGVAYYLAGRALI
                     FLPAVFGIVPLRPDETGTPPIHIELSTLRGLSHVVGVPFNFPVVFVLVPLGLLLLLLL
                     CRVVFRKMWLAVPAALIVASISGVISSTNPALDISANVFSAAVGLLVVFRLGLLPHAI
                     SGLVIATLAVGPLTFDTSAWYAGNTLVVLLIVGGLALYGFKTSLGGRPAFGGGMIPET
                     "
     gene            complement(3694..>5085)
                     /locus_tag="VFD06_13015"
     CDS             complement(3694..>5085)
                     /locus_tag="VFD06_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN04496.1"
                     /translation="RDFRMADYGFQTLIPEPHSFYVYPDGRSMLWYADLEKRAPEIAK
                     FSRKDVDNLARFETDLAELQPFIDDILRMTPPAFPPRGLGEMLDLAKFGRRMMKLGEK
                     KIKHFLELMTSSCADYLGRRFESEEVKAALAINGMIGTCVGPYSPGSAAVMLHHSIGS
                     SIEGQPGAWGYVKGGMSGIANALLGSVKDLGVEVKTDAEVARFIVEDGVCRGVALKNG
                     DDFRARVVASNLDPKRTFLKMIDKGVLDAEFVKDITNFRIQGSSIKMNLALSELPDWK
                     CIPGQDPKAPHQAAMFEIAPSLEYLEMAYDDMKYGRPSKRPLIDGNIASVLDDSLCPK
                     GHHVMSMFVQWGPYHLKGATWPEIREKVGDNILDTLEEYIPNIKKIVIGREVLSPWDL
                     EQIFGLTEGNIFHGEMTPDQLFFLRPAPRWANYRTPVRGLYLCGSGAHPGGGVMGAPG
                     KNAAREILKDIRR"
BASE COUNT          808 a         1721 c         1827 g          729 t
ORIGIN      
        1 gtctgatcga gcgtcacgac gatcgagtcg tccacgagcg gcttcatccg ctcccacatg
       61 cgcgggaaga cgctctggcc gccggcgccg tccacgaacg aggccttcca ctcggggaag
      121 gtctggggcg aacccgcggc cagcgaccgg gacatggggg cggcggtctc cggctcggcc
      181 ccgaagatgc gtacctccgg cttgagcgcc ttgatggcgc tcgcgacccc ggtgatgagc
      241 ccgccgccgc cgatggccgc gatgaccgcg gtcacgtcgg gggcgtcctc gaggatctcg
      301 agccccatgg tggcgtgtcc ggcgatgaac tggtggtcgt cgaacgggtg cacgaacgtc
      361 ccctcggcgc cggggtaggc gcgatcgtcg agggccttcc acgcggtcgt gtaggagacg
      421 aggatcagct tcgccccgag cgccttcatc cgatcgagct tggcctgggg ggcggtctcg
      481 atggcgacga cggtgcaagg gacgccggcg gccctggccg cgtacgccac tccctgtccg
      541 gcgttgccgg cgctgatggt ccagaccccc ttgcgccgtt ccgcatcggt cagggacgcc
      601 accgcattgg cggcgccgcg cagcttgtag gcgttgatcg gctggaggtt ctcgagcttc
      661 aaccggatgt cgggcgcatc ggggcccagc tccagcctga cgagcggtgt gcgcacgacg
      721 gtcttcgcga tgcgctcgcg cgcctcccgg atctcgggca gggtgacggg acggacgggc
      781 tcgggcgtcg taggcatgat gacgatcctc tcgttcccgg agagcatacc gggccccggc
      841 tccagacgca cgatcaagtc tcgggaatca tgcccccgcc gaaagccggt cgcccgccga
      901 gcgaggtctt gaacccgtac agcgccagcc cgccgacgat caggaggacg acgagcgtgt
      961 tccccgcgta ccaggccgag gtgtcgaagg tcagcggccc gacagcgagc gtcgcaatga
     1021 ccaggcccga gatcgcgtgg ggcaggaggc cgagccggaa caccacgaga aggcccaccg
     1081 ccgcgctgaa cacattcgcc gagatatcga gggccgggtt cgtcgacgag atcacgccgg
     1141 agatgctcgc gacgatcagt gcggcgggga ccgccagcca catcttgcgg aacacgacgc
     1201 ggcagaggag caacaggagc agcagtccga gcggaaccag cacgaacacc acgggaaaat
     1261 tgaaggggac ccccacgacg tgcgacaggc cccgcagcgt ggagagttcg atgtggatcg
     1321 gcggcgtccc ggtctcgtcg gggcgcaacg ggacgatgcc gaaaaccgcc ggcaggaaga
     1381 tgagggccct ccccgccagg taataggcca caccgaggac cgccccgaag agcaagtccc
     1441 ggccgatcag cgggtcgcgc agccggccgt cgaggagccg cacccacgac accagcacct
     1501 ggggccagtt gcgtctcaga tagggttcga gggcgagata gaagacccag acgaggccgg
     1561 cgatgagcag acggacggcg agattgatcg tgaagttcgc aaacgcgttg gacgccacgt
     1621 ggtgcgtctc caggagccag gcgatctcgg ccgacaggag catgaacgcg gccagccgga
     1681 aggccccctt gcggtccccg cgtccgaggc ggaggttgcg gcgcgccacg atcactcccg
     1741 ccgacatgat ggagatcgcg acgacgatcc agaccatgga ggcccactcc tggagcacgc
     1801 tcccgggcag ggcggcggtg gccggctcgg agagccgcgc gcgcgtccag ggctcgacgg
     1861 tgcggaaaaa gatcggccgg ccgagatacg aagccgcttc gagccgcagc gtcatccccg
     1921 gcatgcgcgg atcggtcccc tcccatgcgg cgcggcgatc ggcgtacatg cgcggcaccc
     1981 aggacggctc gaccgccttg aacgccgccg gctcgtggcc gcccagcgcg aggagccggt
     2041 tccagtccgg ctcgagagga ggggccgcct gaggatcgcg gtcgggaggg acggcctgga
     2101 atgtccgcag atgcccttcg ggatcgagcc gcagccggac catcccggag tcgatgagcg
     2161 gggggtccga gaaagtgctc ggggtcgcgc ccgcgtcgcc ccacggtttc atcggacggg
     2221 gtgactggcg gtaccagaac gcgatgccgt agggtggacc cgcccttaag gtgtcccagc
     2281 cgggtcgggc ggcctggccc gccgcgagat ggtcgagata ttcgttctcg gggtcgaagc
     2341 ccgacacgct gtctatcgga ggctcgacgt acccgagatc gcgcacgatc tcgcgggccc
     2401 gctcgcgcag gatctcgggg gacttcggca gcggcacgag acgtgcgagc tgcgatcgcc
     2461 ccgacacgaa gacggcggcg gggaggagca ggaggaacgc ggcgagcccc gcccacgcga
     2521 gcgccggctt cagcgcttcc gtcgtccccg cttcggcgac cagctccggc gagggcgtct
     2581 ctccggccgc gagcgccgcg gccagcgggt ctcccccggg aagcgcggcc gccaccgcca
     2641 gggccgtcgc cggccggcgc gccgggtcgc gctcgagaca gcgcaggatg atccgctcga
     2701 cggccgggtc gaatccctcg atgagggccg acggactggt cggcgcggac tccatcttga
     2761 gacgcgccga ctcggcgggg ctcgccgcct tgaacgcctg gtgcccggtg cacagctcgt
     2821 acagcaccat gccgagcgcg tagagatcgc tcttcaccgt caccgccgtg ccggcgatct
     2881 gttccggcgc catgaaggcc ggcgtgccgg cacgcaggtc cgctccctgg atctccccgg
     2941 cgaggcgggc caggccgaaa tcggtgatgc gcgtccggcc gcggccgtcg atcatcacgt
     3001 tcgagggctt cagatcccgg tgcaggatcc cctgctcgtg cgccgcctgg aggccggcgc
     3061 agagctggcg ggcgatctgg atcgccttgt cgcgcggcag ccggtcgatg cgccgcagca
     3121 ggcccgccag gtcctcgccg tcgacgaact ccatcgacag gaagtgctgc ccgtccgcct
     3181 cgccgacgtc gtagacgcgg cagacgttcg ggtggctgat ctgccgcgcc agccggacct
     3241 cttcgaggaa gcgcgccaga cgcccctcgt cccgctcgac ctcccggggg agaaacttca
     3301 gcgcgacggt ctggccgagc ttgaggtcct cggcccggta cacttccccc atcccgcccc
     3361 tgccgaccag cccgaagatt cgataccggc cggccagcat ggtgccgggg aggaagcgtc
     3421 cctccggcgt gccgcccgat cggaaggggg acggatgcgg cgcaatcggc cgcgctggtg
     3481 gatcgtcacg gacggtgcgt gtcggcccgg cggtgcccgg cgcgagcggc gcggcgcagt
     3541 gcaggcagaa acgccccccg tcgggggact cccctcggca cgcgccgcag cggagcatcg
     3601 gcggaggatg cctgaccgtc gccgggaacg caatcgcccg cgcccggcgg aagccggcga
     3661 aaccgccgga ccaacgggcg cgggagaagg gctctagcgg cggatgtcct tgaggatctc
     3721 gcgcgccgcg ttcttccccg gcgctcccat gaccccgccg cccgggtgcg ccccggagcc
     3781 gcacaggtac aggccgcgca cgggggtgcg gtagttcgcc cagcgcgggg cggggcgcag
     3841 gaagaagagc tgatcgggtg tcatctcgcc gtggaagatg ttcccctcgg tcagcccgaa
     3901 gatctgctcg aggtcccagg gggacagcac ctcgcggccg atgacgatct tcttgatgtt
     3961 cgggatgtac tcctcgaggg tgtcgaggat gttgtctccc accttctcgc ggatctccgg
     4021 ccaggtcgcc cctttaagat gatacgggcc ccactgcacg aacatcgaca tgacgtggtg
     4081 ccccttcggg cacagcgagt cgtcgaggac cgaggcgatg ttgccgtcga tgagcggccg
     4141 cttggacggc cggccgtact tcatgtcgtc gtaggccatc tcgaggtact cgaggctggg
     4201 ggcgatctcg aacatcgccg cctggtgcgg cgccttcgga tcctgaccgg gaatgcactt
     4261 ccagtccggc agctccgaca gggcgaggtt catcttgatc gacgaaccct ggatgcggaa
     4321 gttggtgatg tccttcacga attcggcgtc cagcaccccc ttgtcgatca tcttgaggaa
     4381 ggtccgcttc gggtcgaggt tcgaggcgac gacgcgggcg cggaagtcgt cgccgttctt
     4441 cagggcgacg ccccggcaga cgccgtcctc gacgatgaag cgcgcgacct ccgcgtcggt
     4501 cttcacctcg acgccgaggt ccttcaccga gccgagcagc gcgttggcga tgccgctcat
     4561 tccgcccttc acgtaccccc acgcccccgg ctgcccctcg atcgacgagc cgatcgagtg
     4621 gtgcagcatg acggcggcgg atccggggct gtacgggccg acgcaggtgc cgatcatgcc
     4681 gttgatggcg agggccgcct tgacctcttc ggattcgaag cggcggccca ggtagtcggc
     4741 gcacgaggag gtcatcagct cgaggaagtg tttgatcttc ttctcgccga gcttcatcat
     4801 ccgccgcccg aacttcgcga ggtcgagcat ctcgccgagg ccgcgcggcg gaaacgcggg
     4861 cggggtcatg cggaggatgt cgtcgatgaa cggctgcagc tcggcgaggt ccgtctcgaa
     4921 gcgggcgagg ttgtccacgt ccttacggga gaacttggcg atctccgggg cgcgcttctc
     4981 gaggtcggcg taccagagca tggagcggcc gtccgggtac acgtagaacg agtgcggctc
     5041 ggggatgagg gtctggaagc cgtagtcggc catccggaag tcgcg
//
LOCUS       DASRIQ010000531         8974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3882416, whole genome shotgun sequence.
ACCESSION   DASRIQ010000531 DASRIQ010000000
VERSION     DASRIQ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3882416"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFD06_13020"
     CDS             complement(<1..549)
                     /locus_tag="VFD06_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04497.1"
                     /translation="MSLLVLLAAAPGCARGAATLGAASLTVTPGGGIDASTWNTGAFL
                     LDNLSLQGVRITRVRIDLSTALLSDLVFDPLGQAGDTVAKCLTPDSNPGLVGLVQAPD
                     PCSGPFSAPRDDGYQVLTLDFTDFRANRSFLFSVDVDPTLIRGTAAPGPAESGSVSGL
                     EMTGAVVEFTFSDGAVLSAPLFP"
     gene            complement(732..2177)
                     /locus_tag="VFD06_13025"
     CDS             complement(732..2177)
                     /locus_tag="VFD06_13025"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZN04498.1"
                     /translation="MSLFTNRNAALSWKLWPMLAISLAFAGCALSEVALEKGDLGKVK
                     KEQDEPRKVGTGRSFIHETRVDSTPVPAAAPGWFSETVASGQDDWEPAVAVDPANANL
                     VYQLITRYTGPKPCGNCKLPAIILRRSTNGGVSWLPDQWLKQTTKMQFDPQIRVDING
                     NVHAAFLNGTTPGTTYLKSTNQGVTWSAGVDWSGQGRKPQWNDHPWLGISPNGQHVYI
                     GFNSSDSYVVVSHDGGLNFTLPLKMNTDSRYYFHQGVAVTAGGSTAYIATSDFSSGNT
                     YNGDAFVRVWKTTNGGTSWSQVLIDTSRDVPTCGWAAGCYTGFLGSTPNMAIDSAGKL
                     LIAYHVNSTPNVPEQMYIRTSTDGVTWTPRFQISVANPAAHNAFPSVAAHPTVANDFR
                     VTWQSDSVQSQTGWNTFYRRTTDGGVNWSASIDLSDLTSGAPYKNANGYTFPYGDYHD
                     LQVGPDGTNHVFWGEGASYTGPGGCWYARGQ"
     gene            2496..3125
                     /locus_tag="VFD06_13030"
     CDS             2496..3125
                     /locus_tag="VFD06_13030"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZN04499.1"
                     /translation="MTRYRLPDLPYDFGALEPHISGRIMELHHGKHHAGYVKNANETI
                     ARLDEARDSGDTARIAALERALAFNLSGHVLHSIFWQNLTPQGGDKPAGEIGRAIDRD
                     FGSFDRFKRHMVSVASGVMGSGWAALVWEPMAERLMVTQIYDHQSNLNQGSVPLMVID
                     AWEHAYYLQYRNEKEAFFSGLWSLWNWDDITARFAAAWQLDLALVHQDR"
     gene            complement(3064..4719)
                     /locus_tag="VFD06_13035"
     CDS             complement(3064..4719)
                     /locus_tag="VFD06_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04500.1"
                     /translation="MSSAVPPPSTGFRARPAVATPLLGAALAAAAVFLLSLPYLPFQD
                     LPNHALLLIYDRALGPEGNAWLQRPSFISFGYTAYIWWARLLAPVLSIDGVLKLLAAM
                     AVAALPLATARLAAVLGAPGALAGLLVLPFALGWPLRMGLISFVLGLPLALLCAASAV
                     LLGRRPSTGRALILALECAAAYLTHAFAFGLALALILLAVAVPGRPSRRLAGMLVLAL
                     LPAGILVGWDAWHGSWRPTGSASPISAPESPVRFRPVGEALSHVACRTYGIPRASSLP
                     SYLPHLALLAIAAGTLLARRRGAGESLDPAARRFLLAGGTILTLGTLAMPDTAGRAYL
                     LGSRPAMAGMCLLGIAAAVGLRRTVRRGALPAAAAATILACAASVAGIARDAREVRDV
                     VGRRPPRSVAGNVLVARAADCRRATSYAWGAWDPLRQAWAYALSPAGSTPYLFAEHRY
                     DMVWYRPGANPPHPPAGRALSDERNLDGAACAERNRERVLRSLSWPGYDGVIVLGSPE
                     AGRAAVEASGVRDPVRLAPGMWLLSGPGAPARDRAATRPRSGP"
     gene            4812..5099
                     /locus_tag="VFD06_13040"
     CDS             4812..5099
                     /locus_tag="VFD06_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HZN04501.1"
                     /translation="MNRVDAITVEVPGPLRAASGGAARVSVPAKTLRGVLEELERRYP
                     ALHRSICDETGKVRPHVNLFVNQQHMRDRGGLDTALGPGDVVIILPAVSGG"
     gene            5102..6214
                     /locus_tag="VFD06_13045"
     CDS             5102..6214
                     /locus_tag="VFD06_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HZN04502.1"
                     /translation="MPDRVLLAIGTKKGLFVAEGSKTRRSFDLRGPFSPGVAVYSALI
                     DARGKPTVYASSCNAFFGMKVLRSTDKGKSFKETKSAPAFPKEDGRALANIWSLAPGE
                     GKKDLWAGVEPAALFRSHDGGDTWTLSEGLSNHPHARQWQPGNGGLCLHTLIPDGKRL
                     HAGISTGGHYVSDDGGATFAASNKGVGAGFSPDPYPEFGQCVHKIARHEDAKDRLYMQ
                     NHGGWGEWDGPGGRRPDIGVLRSDDGGRSWRSIAKGLPSDFGFPIVVHPHDPDTIYVV
                     PLEPSTRTCPGGSPAVWRSEDGGGSWKRLARGFPKKGSYFTVLRDAMDVDRLKAPSLY
                     FGTTTGQLWIGRDGGDSWDCLFDSLPPIHCVKVAVV"
     gene            complement(6284..7456)
                     /locus_tag="VFD06_13050"
     CDS             complement(6284..7456)
                     /locus_tag="VFD06_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HZN04503.1"
                     /translation="MRRLLSVLCSCSVGLLAVLAMLALGSTEAWAGKSGKSGGGGKVC
                     LCHVPPGNPGNAHTICVGAPAVQAHLNHGDSLGECSSSAGCGGEAGIACPTGQFCQRD
                     VGICAASAEGVCIALPASCPTTLAPVCGCNETTYDNDCLADLAGVAVLHTGECVCEGD
                     TPTACGGTEGVTCATGEFCEKPDGACAADAEGVCRPTPGTCPPDFIPVCGCDGTTYSN
                     ACFADVSGVPVNTTGACPATACGGTGGATCPTGQFCLPPVGTCTAGAEGLCVPTGPLV
                     CPDVLDPVCGCDGTTYGNACGARSAGVAINHTGPCEGETVCGGTAGTPCATGSYCSKE
                     EGACAADAEGVCAPILPACPAVIDPVCGCDGTTYDNACVANAAGVTIASQGACPAP"
     gene            7616..8140
                     /locus_tag="VFD06_13055"
     CDS             7616..8140
                     /locus_tag="VFD06_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04504.1"
                     /translation="MRAFQEGRGTPALAIAILIAAASLRCATTTAAHAPTLTWEQHTL
                     RIPLKVEAQGTVSFAERPPLLGRLDLTPILEAEGLKPGKPALTAQAMMHYGRLYIVAE
                     KFRSIWEVTPQPGTSRASYRPIPVLPETAPGRLKDVRLSRYGSSKSSCLRLDRAEGPP
                     VFITSAGEARHDCP"
     gene            8127..>8974
                     /locus_tag="VFD06_13060"
     CDS             8127..>8974
                     /locus_tag="VFD06_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04505.1"
                     /translation="MTARRAIAASIVAAAMLAAQARQEADPHELYERATEHLEQGRLQ
                     ESIAASTRLRELVAARSEWDPDGAFRRDLLPPLEARQKRLLSAARALDQFHDRSLEEL
                     RPPDLSSEISTVRDYTHWATTVVQRLRKERDTLVEGILTDPADAAILARTESWARSER
                     LLEVEVLKTMAEKAGDDVLGLLSGDAQLESVLVRFRQLKKDLIQAMVERDRFQAQIKE
                     AEERRQALAAAVAAAVLEMEGPKKGRRDEATGVQARFAHLLKSEREALQKRPSVTPAE
                     QAALEER"
BASE COUNT         1396 a         3060 c         3246 g         1272 t
ORIGIN      
        1 cgggaagagc ggcgcgctca ggaccgcccc gtcgctgaag gtgaattcga cgacggctcc
       61 ggtcatctcg agccccgaga cactcccgga ttcggcggga cccggcgccg ccgtcccgcg
      121 gatgagagtg ggatcgacgt ccaccgagaa gaggaacgag cggttggcgc ggaaatcggt
      181 gaagtcgagc gtcagcacct ggtagccgtc gtcgcgcggc gcgctgaagg gacctgagca
      241 gggatcgggg gcctggacca gaccgacgag accggggttc gaatcgggcg tcaggcattt
      301 ggcgaccgtg tcgcccgcct gcccgagcgg atcgaagacg aggtcggaga gcagcgccgt
      361 ggagagatcg atccgcaccc gggtgatccg cacgccttgc agtgagaggt tgtcgaggag
      421 gaacgcgccg gtgttccagg tgctggcgtc gatcccgccc cccggcgtga cggtgaggga
      481 ggccgctccc agggtggcgg cgcccctggc gcaaccaggg gccgccgcca ggagaacgag
      541 gaggctcact gccgccgggg cggcgcgtcg caggaggtcg gggatcacgc tcgggaagac
      601 cggcccccgc gaggcgaatc aaaacgatgc ggggcccggc tcaagagatg ccgtgagccg
      661 ggccccgtgt caatcgaaag tcccgcgggc gcgcctgatt tcgcgcccgc cgtcaatgcg
      721 ttgacttgct gttattgccc gcgggcgtac cagcagccgc cggggccggt gtacgaggcc
      781 ccctcgcccc agaacacgtg gttcgtgccg tccggcccga cctgcaggtc atggtagtcg
      841 ccgtacggga aagtgtagcc attggcgttc ttgtacggcg cgccgctcgt gaggtcggac
      901 aagtcgatgg aggcgctcca gttgacgccg ccgtcggtcg tccggcggta gaaggtgttc
      961 cagccggtct ggctctggac cgaatcgctc tgccaggtga cccggaaatc gttggcgacg
     1021 gtcgggtgcg ccgccaccga cgggaaagcg ttgtgcgccg ccgggttggc gaccgagatc
     1081 tggaaccgcg gggtccaggt cacgccgtcg gtcgaggtcc ggatgtacat ctgctccggc
     1141 acgttcggcg tgctgttgac gtgataggcg atcaggagct tcccggcgct gtcgatcgcc
     1201 atgttcggcg tcgagccgag gaagccggtg tagcagccag ccgcccagcc gcacgtcggc
     1261 acgtcgcggg atgtgtcgat gagcacctgg gaccacgacg tgccgccgtt ggtcgtcttc
     1321 cacacgcgga cgaaggcatc gccgttgtag gtgttgccgc tggagaagtc gctcgtcgcg
     1381 atgtaggcgg tgctgccgcc ggcggtgacc gcgaccccct ggtggaagta gtagcggctg
     1441 tcggtgttca tcttcaacgg gagtgtgaag ttgaggccgc cgtcgtgcga cacgacgacg
     1501 tacgagtcgc tcgagttgaa gccgatgtag acgtgctggc cgttgggtga aatcccgagc
     1561 cacggatggt cgttccactg cggcttccgg ccttgcccgg accagtcgac gccggccgac
     1621 caggtcacgc cctgattggt cgatttgagg taggtggtgc ccggcgtggt gccattgagg
     1681 aacgcggcgt ggacgttgcc gttgatgtcc acgcgaatct gcggatcgaa ctgcatcttc
     1741 gtcgtctgct tgagccactg gtccggcagc caggagacgc cgccgttggt cgagcggcgc
     1801 aggatgatcg ccggcagctt gcagttgccg cacggcttcg gcccggtgta gcgcgtgatc
     1861 agctggtaga cgaggttggc attggccgga tcgacggcca ccgccggctc ccagtcatcc
     1921 tgcccgctcg cgacggtctc cgagaaccag ccgggggcgg ccgccggcac gggcgtcgag
     1981 tccacccgcg tctcatggat gaacgacctg ccggttccga cctttctcgg ctcgtcctgt
     2041 tccttcttga ccttccccag atcacccttt tccagcgcca cctccgagag ggcgcagccc
     2101 gcgaaggcca gtgagatggc cagcatcggc cagagtttcc acgacagggc ggcgttccgg
     2161 ttcgtgaaca ggctcatcgc gttccctcct tggtgggtgg tgcaggtcgg gcgtgatggg
     2221 gatgcgtgcg gccgtggcac gagcgtgacg gtcgagtcgg gaccgcgttg cgaatcatga
     2281 aacgtccccc ggcgagtcct agcgcgggat agtactcggt tgagcggaat tgtgcatcga
     2341 cgacgggatt tttttcgatc cgcgcctggg caggctgttc ggggaggtgg gccgcgtgga
     2401 aagactccgc gatcggcggc gcggcctctg cgggattcta ccgaccgatt tgaagcctcc
     2461 gcgaatcgca cggatcatgg gcgaggaggc ttcgaatgac tcgatatcga cttcccgatc
     2521 ttccctacga cttcggcgcg ctcgagccgc acatcagcgg gcgcatcatg gagctgcacc
     2581 acggcaagca ccacgccggc tacgtgaaga acgccaacga gacgatcgcg cggctggacg
     2641 aagcgcgcga ctccggcgac acggcgcgga tcgcggcgct cgagcgcgcg ctcgccttca
     2701 acctctcggg gcacgtcctg cactcgatct tctggcagaa cctcacgccg cagggcggcg
     2761 acaagccggc cggcgagatc gggcgcgcca tcgaccgcga cttcggctcg ttcgatcggt
     2821 tcaagcgcca catggtgtcg gtcgcctccg gcgtcatggg atccggatgg gcggcgctcg
     2881 tctgggagcc gatggcggag cgcctgatgg tcacgcagat ctacgaccac cagtcgaacc
     2941 tcaaccaggg gagcgttccc ctcatggtga tcgacgcatg ggagcacgcc tactacctgc
     3001 aataccgcaa cgagaaggag gcgttcttca gcggcctgtg gagcctctgg aactgggacg
     3061 acatcacggc ccgcttcgcg gccgcgtggc agctcgatct cgcgctggtg caccaggacc
     3121 gctgaggagc cacatccccg gagccagccg gaccgggtcg cgtacgccgc tcgcctcgac
     3181 ggcggcgcgg cccgcctccg gagatccgag gacgatcacc ccgtcgtagc cgggccagct
     3241 caggctccgc agcacgcgct cgcggttccg ctcggcgcac gccgcgccgt cgaggttgcg
     3301 ctcgtcggac agcgcgcggc cggccggagg atgcggcggg ttcgccccgg gccggtacca
     3361 gaccatgtcg tagcggtgct ccgcgaacag atacggcgtg ctcccggcgg gagacagggc
     3421 gtaggcccag gcctggcgga ggggatccca tgcgccccat gcgtacgagg tcgcgcgccg
     3481 gcagtcggcg gcgcgcgcca ccaggacgtt gccggcgacg ctgcgcggcg gacgccttcc
     3541 gaccacgtcc cggacctcgc gcgcgtcgcg cgcgatcccg gcgacgctgg cggcgcaggc
     3601 caggatcgtc gccgccgccg cggccggcag cgccccgcgc cggacggtcc gtcgcagtcc
     3661 gacggcagcc gcgatcccca ggaggcacat gccggccatc gccggccggc ttccgaggag
     3721 atatgcgcgt cctgccgtgt ccggcatcgc cagtgtgccg agcgtcagaa tcgtccctcc
     3781 cgcgaggagg aacctccgcg ccgcgggatc gaggctctcg cctgcgccgc gccggcgggc
     3841 cagaagcgtt cccgcggcga tggccaggag cgcgagatgc gggaggtagg agggcaggct
     3901 cgaagcccgc gggatgccgt aggtgcggca ggcgacgtgc gacagcgcct cccccaccgg
     3961 gcggaagcgc accgggctct ccggagcgga gatcggggag gccgacccgg tcgggcgcca
     4021 cgagccgtgc cacgcgtccc agccgacgag gatcccggcc ggcagcagcg ccaggaccag
     4081 catccccgcc aggcgccgcg agggccggcc cgggaccgcc accgcgagga ggatgagggc
     4141 cagcgccagg ccgaaggcga aggcgtgcgt caggtaggcg gcggcgcact cgagcgccag
     4201 gatgagggct cgtcctgtcg agggacgccg gccgaggagc accgcggacg cggcgcacag
     4261 cagggcgagc gggagtccga gcacgaacga gatgaggccc atccggagcg gccatccgag
     4321 ggcgaacggc aggacgagca ggccggcgag cgcccccggg gcgccgagca ccgccgccag
     4381 gcgcgcggtc gccagcggca gcgcggcgac cgccatcgcc gccagaagct tcagcacccc
     4441 gtcgatcgac aggaccggcg ccagaaggcg cgcccaccag atgtaggcgg tgtatccgaa
     4501 cgagatgaac gaggggcgct gcagccaggc gttcccttcc ggtcccagcg cccggtcgta
     4561 gatcagaagg agcgcgtggt tcggcagatc ctggaacggc aggtagggga ggctcagcag
     4621 gaacactgcg gcggcggcga gcgcggcccc cagcagcggg gtcgctacgg ccggccgggc
     4681 gcggaagccg gtcgagggcg gggggacggc ggaggacatg acgcttccgg aagctcgggg
     4741 tctccactcc gagcaaacgc tccggcaggc cgcttcgttc cgtggcatac tcccggcttc
     4801 ctgcagggcc agtgaaccga gtggacgcga tcaccgtgga agtcccgggg ccactccgcg
     4861 ccgcgagcgg cggggcggcc cgcgtctccg tgccggcgaa gaccctgcgg ggggtgctcg
     4921 aggagctgga gcgccgctac cccgccctgc accgcagcat ctgcgacgag accgggaagg
     4981 tccgcccgca cgtcaacctg ttcgtgaacc agcaacacat gcgcgaccgc ggagggctcg
     5041 acacggcgct cggaccgggc gacgtcgtca tcatcctccc tgcggtctcg ggaggctgaa
     5101 gatgccggat cgcgtgctac tcgccatcgg aacgaagaag gggctgttcg tcgcggaagg
     5161 gtcgaaaacc cgccggtcgt tcgatctgcg cggcccgttc tcccccggcg tcgccgtgta
     5221 ctcggcgctc atcgacgcgc gcggcaagcc gaccgtctat gcctcgtcct gcaatgcctt
     5281 cttcgggatg aaggtcctgc gctcgaccga caaggggaaa tcgttcaagg agacgaagtc
     5341 ggccccggct ttcccgaaag aggacggccg ggcgctcgcc aacatctggt ccctagcgcc
     5401 cggcgaaggg aagaaggacc tctgggccgg ggtcgagccg gcggcgcttt tccggagtca
     5461 cgatggcggc gacacctgga cgctgtcgga agggctgtcg aaccacccgc atgcgcgcca
     5521 gtggcagccg gggaacggcg gcctgtgtct gcacaccctg atcccggacg gcaagcggct
     5581 gcacgccggg atctcgaccg gcggccacta cgtcagcgac gacggcggcg ccaccttcgc
     5641 ggcctccaac aagggggtcg gcgccggctt cagccccgat ccgtaccctg agttcggcca
     5701 gtgcgtgcac aagatcgccc gccacgagga cgccaaggac cggctctaca tgcagaacca
     5761 cggcggctgg ggggagtggg acggtcccgg cggccggcgc cccgacatcg gcgtgctccg
     5821 cagcgacgac ggcggccgct cgtggcgctc gatcgcgaag gggcttccgt cggacttcgg
     5881 cttcccgatc gtcgtgcacc cgcacgaccc ggacacgatc tacgtcgtgc cgctggagcc
     5941 ttcgacccgc acctgcccgg gcggctcccc cgccgtctgg cgcagcgagg acggcggcgg
     6001 atcgtggaaa cgcctggcca ggggattccc gaagaagggg tcctacttta cagtgctgcg
     6061 cgacgccatg gacgtggatc gcctgaaggc gccgtcgctg tatttcggca ccacgaccgg
     6121 ccagttgtgg atcggccgcg acggcggcga ttcgtgggac tgcctgttcg actcgctgcc
     6181 gccgatccac tgcgtcaagg tcgcggtggt ctgaggacgc gccccggcag cgatggcccg
     6241 gcgccccgcg cggacgcggg gcgccgggat ggccttcgtt ctgctacggg gccgggcagg
     6301 cgccctggct cgcgatcgtc acccccgccg cattcgcgac gcaggcgttg tcgtacgtcg
     6361 tgccgtcgca gccacacacc ggatcgatca ccgcggggca ggcgggcagg atcggcgcgc
     6421 acactccttc ggcatcggcg gcgcaggccc cctcttcctt gctgcagtag gagccggtgg
     6481 cgcacggggt gcccgcggtg ccgccgcaca ccgtctcacc ttcgcaggga ccggtgtggt
     6541 tgatcgcgac gcccgcgctt cgggcgccgc aggcgttgcc atacgtcgtt ccatcgcagc
     6601 cgcagaccgg gtctagaaca tccgggcaga ccagcgggcc ggtcggcacg cagagcccct
     6661 cggctccggc cgtgcaggtc cccactggag gcaggcagaa ctgccccgtg ggacaggtcg
     6721 ctcccccggt gccgccgcac gccgtggccg ggcaggcgcc ggtggtgttc accgggacgc
     6781 cggagacatc ggcgaagcag gcgttgctgt aggtcgtgcc gtcgcagccg cacaccggga
     6841 tgaagtctgg cgggcaggtg cccggcgtcg gcctgcagac cccctcggcg tcggccgcgc
     6901 acgccccgtc cggcttctcg cagaattctc ccgtcgcgca ggtgacgccc tcggtgccgc
     6961 cgcaggcggt cggagtgtcg ccctcgcaga cgcattcccc ggtgtgcagg accgcaacac
     7021 cggcgagatc ggcgaggcag tcgttgtcgt acgtcgtctc gttgcagccg cagaccggtg
     7081 cgagcgtggt cgggcaggag gccgggagcg cgatgcagac cccctccgcg cttgcggcgc
     7141 agatccccac gtctctctgg cagaactggc ccgtcggaca ggcgatgccc gcctctccgc
     7201 cgcagccggc ggaggagctg cactctccca gcgaatcgcc gtggttgaga tgtgcctgga
     7261 ccgccggcgc gccgacgcag atggtgtgcg cgttgcctgg gttccccggt ggcacgtgac
     7321 acaggcagac cttgccgccg ccacccgact tccccgactt cccggcccag gcttcggtgg
     7381 aaccgagggc gagcatggcg agcacggcga gcagaccgac gctacaggaa cagaggaccg
     7441 acaggagccg tctcatgggt tcctccccgt acggatgtga ttgtgtcagg gagcctatcc
     7501 caaaatcgtc acgccctcca agggtttctg aggatttccc ctcggggtga cgatttcgtg
     7561 atcgtacgta attccgcagg ttgcgaatca atcagcggcc accaagattg aacgggtgcg
     7621 cgccttccag gagggccggg gaacccctgc gctcgcgatc gccatcctga tcgccgcggc
     7681 gagcctccgc tgcgccacga ccaccgccgc gcatgcgccg acgctcacct gggagcagca
     7741 cacgctgcgc atcccgctca aggtggaggc ccaggggacg gtgagcttcg ccgagcgccc
     7801 gccgctgctc ggccggctcg acctcacgcc gatcctcgag gccgaggggc tcaagccggg
     7861 caagccggcg ctgaccgcgc aggcgatgat gcactacggc cggctgtaca tcgtcgccga
     7921 gaaattccgc tcgatctggg aggtgacgcc gcagccgggg acctcgcgag cgtcgtaccg
     7981 cccgatcccg gtgctccccg agacggcgcc cggccggctc aaggacgtgc gcctgtcccg
     8041 ctacggctcg tcaaaatctt catgtctgcg cctcgatcgt gccgagggcc ctccggtgtt
     8101 catcacctct gccggtgagg cgcgccatga ctgcccgtag ggcgatcgcc gcctcaatcg
     8161 tcgcggcggc gatgctcgcc gcgcaggcgc gccaggaggc cgacccgcac gagctgtacg
     8221 agcgggcgac ggagcacctc gagcaggggc ggctgcagga atcgatcgcg gcctcgacgc
     8281 ggctgcgcga gctggtcgcg gcgaggtcgg aatgggatcc cgacggcgcc ttccgccgcg
     8341 atctcctccc gccgctcgaa gcgaggcaga agcgcctcct gagcgccgcg cgcgctctcg
     8401 accagttcca cgatcggtcg ctcgaggagc tccggccgcc ggatctcagc agcgagatct
     8461 cgaccgtccg ggactacacg cactgggcga ccaccgtggt gcagcgcctg cggaaggagc
     8521 gggacaccct cgtcgagggg atcctgaccg acccggccga cgccgcgatc ctggcgcgca
     8581 ccgagagctg ggcgcgcagc gagaggctgc tcgaggtcga ggtgctgaag acgatggcgg
     8641 agaaggcggg ggacgacgtg ctgggccttc tgtccggcga cgcccagctc gaatcggtcc
     8701 tggtccgctt ccgccagctg aagaaggacc tgatccaggc gatggtggag cgggaccgtt
     8761 tccaggccca gatcaaggag gcggaagagc ggcggcaggc cctcgcggcc gcggtcgccg
     8821 cggccgtcct ggaaatggaa gggccgaaga agggacgacg cgacgaagcg acgggagtgc
     8881 aggcccgctt cgcgcacctg ctgaaatcgg agagggaggc gctgcagaaa cgaccgtcgg
     8941 tcacccccgc ggaacaggcg gcactcgagg agcg
//
LOCUS       DASRIQ010000532         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_455469, whole genome shotgun sequence.
ACCESSION   DASRIQ010000532 DASRIQ010000000
VERSION     DASRIQ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455469"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2454
                     /locus_tag="VFD06_13065"
     CDS             <1..2454
                     /locus_tag="VFD06_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04506.1"
                     /translation="YKTTDGGVSWGVLPSTAGMSGVPANTIFIEPIAPSRLWVGNDNG
                     IYRSENSGSSWAKYSKGLPNVPVYGFARNKDKALIAATHGRGAWTMTLYNIAPNGTTP
                     VNNVLYNVAALGQGFAANQACALAVVRQDGTVCASGPNDALGGVVSTDERGFLVSSKP
                     GFFSRTPVVWACAQGKCLGKDVMDCNLERNPMAFLQATCGGQATRGTIAPETSAPDPP
                     GAVLGIGWLAPAGGGAPPAVSGSFTIVPAVQSGDGATRRLCSAAVTVSPADNAAAVLG
                     RAAAAINADPTCRLAGVNARLDSGDPGDGGEDRFPGVDRLVLEGPLLTGSRLVPAVRT
                     GAGATTGLCFRMGGLGHVSEMAGLRIRFTTPTSGSHGGSLHVIEQSPLGRCEIVATIP
                     PGAQETGVAQAVAAAFQASDAGNPNPGCPAQVNPRDVTQQGSSVLAAFASEMEVCLDD
                     AAVGVSVAPEEICFAAADCDDGNPCTADACDPITAQCSHAAAPNGTPCADADLCTTGE
                     TCSGGVCGTRVVCSDGNRCTEDACNPATGGCVSAPVVCDDGNACTHDACDAGSGQCVF
                     APLPAGTTCDDGDPCSTADACVTVTGQPNPTCSGTSACDDSDSCTSDQCDTDNGACVF
                     LPIQCDDGNPCTIDQCEAGVCRSSGVAGVACDDGNNCTSGDTCAFHPANGQFSCDGNA
                     VDCVDRDICTADACDPVTGACGHVPVVLAPVPLLNLLDTTRVGWSAVAGALTYNSYRG
                     TIPAQMLGSRPLPRYDQTCFEAHDAAGDGALVSNDPQVPPLGTAFYYLASERTGCGES
                     AIGEDSNTTTIPNTAPCPP"
     gene            2704..>3439
                     /locus_tag="VFD06_13070"
     CDS             2704..>3439
                     /locus_tag="VFD06_13070"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZN04507.1"
                     /translation="MRNRFGVMSGVTLSLAVLVGGAALGVKPAWAQAATAGGAPEGFE
                     WGYNKGFFFRNPNFELKISTRTQFRYTYRMLETDATDRDNGAFNIPRARFRLDGYAWQ
                     PWFKYKLQYDFVGARDLCPSGGVGCDALLQRSDLRDLYFDVTKQPWYQVRMGQFKQPF
                     GQQELTSSGDQQFVDRSIASTVFAPSREIGTMLYGLSFEKVFGYEVAVSNGNGRNHSA
                     DNNSGYRWSARIHWDPNGEYKLSESAV"
BASE COUNT          526 a         1201 c         1219 g          493 t
ORIGIN      
        1 tacaagacga ccgacggcgg tgtgagctgg ggcgtcctcc cctcgaccgc gggcatgagc
       61 ggcgtccctg cgaacaccat cttcatcgag ccgatcgcgc cttcgcggct gtgggtcggg
      121 aacgacaacg ggatctaccg gagcgagaac agcggctcgt cgtgggccaa gtacagcaag
      181 ggtctgccga acgtgccggt ctacggcttc gcccggaaca aggacaaggc cctcatcgcc
      241 gcgacgcacg ggcgcggcgc ctggacgatg acgctttaca acatcgcgcc gaacgggacg
      301 actcccgtga acaacgtcct gtacaacgtg gcggcgctcg ggcaggggtt cgcggcgaac
      361 caggcgtgcg cgctggcggt cgtccgccag gacggcaccg tgtgcgcctc gggtccgaac
      421 gacgcgctcg ggggtgtcgt gagcaccgac gagcgggggt tcctcgtttc gagcaagccc
      481 ggcttcttct cgcggacgcc ggtcgtctgg gcctgcgcgc aggggaagtg cctcggcaag
      541 gacgtcatgg actgcaacct cgagcgcaat ccgatggcgt ttctgcaggc gacgtgcggc
      601 ggccaggcca ccaggggaac gatcgcgccg gagacgtccg ctccggatcc gccgggggca
      661 gtgctgggga tagggtggct cgcgccggcg ggcggagggg cgcctccggc ggtcagcggg
      721 tcgttcacga tcgtgccggc ggtccaatcg ggggatggcg ccactcggcg cctgtgctcg
      781 gccgccgtca ccgtctcgcc ggccgacaac gccgccgccg tcctcgggcg cgcggccgcc
      841 gcgatcaatg ccgatccgac ctgccgcctc gccggcgtga acgcccggct ggactccggc
      901 gaccccggag acgggggaga ggatcgcttc cccggggtgg atcggctcgt cctcgagggg
      961 ccgcttctga ccgggagccg gctcgtccct gcggtgcgga ccggcgcggg cgcgacgaca
     1021 gggctctgct tccggatggg tggtctcggt catgtctccg agatggccgg gctgcggatc
     1081 cggttcacga caccgacgag cgggtcgcac ggcgggtcgc tgcacgtgat cgagcagtcg
     1141 cccctggggc gctgcgagat cgtcgcgacg attccgccgg gggcgcagga gaccggcgtc
     1201 gcgcaggcgg tggccgccgc gttccaggcg tcggacgccg ggaaccccaa tccgggctgc
     1261 ccggcgcagg tcaacccgcg ggacgtgacg cagcagggga gttcggtgct cgcggcgttc
     1321 gcctccgaga tggaggtgtg cctggacgac gcggccgtcg gagtgtcggt ggcgccggag
     1381 gagatctgct ttgccgcggc cgactgcgac gacgggaatc cctgcaccgc cgacgcctgc
     1441 gacccgatca cggcgcagtg ctcgcatgcg gccgcaccga acgggacgcc ctgcgccgac
     1501 gccgatctct gcacgacggg ggaaacctgc tcgggcggcg tctgcggcac gcgcgtcgtc
     1561 tgcagcgacg ggaaccgctg taccgaggac gcctgcaacc cggcgacggg agggtgcgtg
     1621 agcgctcccg tggtgtgcga cgacggcaac gcctgcacgc acgacgcctg cgacgcgggg
     1681 tcggggcagt gcgtgttcgc gccgctcccg gccgggacga cgtgcgacga cggcgatccg
     1741 tgctcgacgg cggacgcctg cgtgactgtg acgggccagc cgaatccgac ctgcagcggc
     1801 acgtccgcct gcgacgactc cgattcctgc accagcgacc agtgcgacac cgacaacggc
     1861 gcgtgcgtgt tcctgccgat ccagtgcgac gacggcaatc cctgcacgat cgatcagtgc
     1921 gaggccgggg tgtgccggtc gtcgggcgtg gccggggtcg cctgcgatga cggcaacaac
     1981 tgcacctccg gcgacacctg cgcgttccac ccggccaacg ggcagttctc ctgcgacggg
     2041 aacgccgtcg actgcgtgga ccgggacatc tgcaccgcgg acgcctgcga tccggtcacg
     2101 ggcgcctgcg gccacgttcc agtcgttctc gcccccgtgc cgctcctgaa ccttctcgac
     2161 accacgcgcg tcggctggtc cgccgtggcc ggggcgctga cgtacaactc ctaccgcggc
     2221 accatcccgg cgcagatgct cgggagccgg ccgcttccgc gctacgacca gacctgcttc
     2281 gaggcgcacg acgccgccgg cgacggagcg ctggtttcga acgatccgca ggtgcctccg
     2341 ctcggcacgg ccttctacta cctcgcgtcg gagaggaccg gatgcggcga gagcgccatc
     2401 ggagaggact cgaacaccac gacgattccg aacaccgccc cctgcccgcc gtagcccgat
     2461 cgcacaaaga ccaggcgcgc gacaggacct gaagcggctc aagtcgcgtt cgcttcggga
     2521 cgaccattcc cgccactgca ccattggcgc tgtcacgcgt ccttaaccgg cccgtcacgt
     2581 tgtcacccat ccgtcacgtc tccgacaccg gcgcgatccg cgtccgggac aaatctcccg
     2641 ccgcgcgcgg ggaactccac cgcgcatcga gacccccgag cggggggcgg gaggaaggcg
     2701 gcgatgcgaa accgtttcgg agtgatgtcc ggcgtgacac taagtctcgc cgtgctggtc
     2761 ggcggcgcgg cgctcggggt gaaaccggcc tgggcgcagg cggccacggc cggcggggcg
     2821 ccggaagggt tcgagtgggg ctacaacaag gggttcttct tccggaaccc gaacttcgaa
     2881 ctgaagatct cgacccgcac gcagttccgc tacacctacc gcatgctcga gaccgacgcg
     2941 accgatcggg acaacggcgc cttcaacatc ccgcgcgccc gtttccgcct cgacggctac
     3001 gcctggcagc cgtggttcaa gtacaagctc caatatgact tcgtcggcgc ccgcgacctg
     3061 tgtccctcgg gcggggtcgg gtgcgacgcc ctgctgcagc gctccgacct gcgcgacctg
     3121 tacttcgacg tcaccaagca gccctggtac caggtgcgga tggggcagtt caagcagccg
     3181 ttcgggcagc aggaactcac ctcttcggga gaccagcagt tcgtcgatcg ctccatcgcc
     3241 tcgacggtgt tcgcgccgtc ccgcgagatc gggacgatgc tctacgggct gtcgttcgag
     3301 aaggtgttcg ggtacgaagt ggcggtcagc aacggcaacg gtcgcaacca cagcgccgac
     3361 aacaattccg gctaccggtg gagcgcccgg atccactggg acccgaacgg cgagtacaag
     3421 ctgtcggaga gcgccgtcg
//
LOCUS       DASRIQ010000533         5288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1776138, whole genome shotgun sequence.
ACCESSION   DASRIQ010000533 DASRIQ010000000
VERSION     DASRIQ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776138"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFD06_13075"
     CDS             complement(<1..531)
                     /locus_tag="VFD06_13075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN04508.1"
                     /translation="MILERVEHPGWLSNTWIAAPREDGEAVLVDAGGPAGPVLDLLRR
                     HRLAVTHILLTHHHGDHVAERDALRRATGARVLAHPLEARRLGGVDLEVADGVDVETG
                     SMRIRALHIPGHTDGQTAWLVDDTVCFTGDTLFRGSIGSTTAPGHTDFADLKRSLLDR
                     LLALPDEVIVAPGHSSP"
     gene            complement(538..1218)
                     /locus_tag="VFD06_13080"
     CDS             complement(538..1218)
                     /locus_tag="VFD06_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN04509.1"
                     /translation="MPKVLVVEDDDSMAAALNDGFRYEGYEVTLARDGADGLRLAGEC
                     APDLVILDVMLPKMSGLDVCKEIRRAGSSVPIIMLTARGQEIDKVLGLKLGADDYVTK
                     PFGFMELMARVEALLRRSTGGGAQAEAYRFGDVMVDFRKAELRKKGKLVEMSARELRL
                     LQYFVNHRGEVIQRDRLLDEVWGYEEAPLTRTVDMHVAKLRKKIENRPSDPQFLLTVH
                     GLGYKFTG"
     gene            complement(1234..2901)
                     /locus_tag="VFD06_13085"
     CDS             complement(1234..2901)
                     /locus_tag="VFD06_13085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN04510.1"
                     /translation="MSDGGWGTRAAAFVRRHTLWAGLVGVLVPLLVLLVLQLVQLRQL
                     REMQAIARRATLDNYLEAIGTEVQYFYRSAAERSLNLPAGWFIQDRLDLVAGQWTQKP
                     IKGASRMFVVDFTKLDTGEVRVLDPRGQRFIKVSADEETMAILSATTPWQMARMQKHP
                     VEGGILTVEERNPDFRVILNSITSDDHQVAAVAGFIVDIGFFRKKLLPAVIQKSLPEF
                     FPGETGKDLEVMIKDGQGRLILGEEHPALPGEVVTSRFPFVFTDWTLILHSHRSTPEH
                     FASVNFAINASLLLLLGGALMISLVLALRAADRAMRLSTMKADFVSNVSHELRTPLAS
                     IRVFAELLRLGRVQTPEKVREYGEFIERESRRLSRLIENILDFSRIESGRKTYTFAAT
                     DVRQVVEGVLQSFDVHLKHHGFTARLEAPEGALPEVLADADALGQAVHNLLDNAVKFS
                     GEARSIEVLLGAGDGEVTIAVRDRGIGVPLDEQAKIFDRFHRVSTGLVHEVKGSGLGL
                     SLVHHIVTVHGGRVSLESAPGRGSTFILHLPARRPPADEALPRSGTV"
     gene            3216..3749
                     /locus_tag="VFD06_13090"
     CDS             3216..3749
                     /locus_tag="VFD06_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN04511.1"
                     /translation="MTRSAKWIALVALTLLVLAPLAVAGEGKDCGDKSAAAAKETHAS
                     KKCSYGTQDCLDHMAAKMKTGGWVGIELEKSETSGVMTVARVVPGSPAEQAGFQVGDV
                     LFALNGIEINEKNESALKAAKKDWKPGQNVNYTLKRGGQSVNANLTLAAMPADVLAKF
                     IGDHMLEHASTEVVASK"
     gene            3904..4494
                     /locus_tag="VFD06_13095"
     CDS             3904..4494
                     /locus_tag="VFD06_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZN04512.1"
                     /translation="MIVVRRILAGAGLSLLLAWSCTPAGQHPQPGHGARAEKPSQPSP
                     SPTPKPNIAQKAPVPPKHKCPYSTQECLNHMASRLQASGWIGIEYEPEEGGVITVQRV
                     VPGSPAEKAGLTVGDVLFSINGVEIRKGNDAAMKKAKVDYRPGQKVHYVIKRNGASKP
                     IDIVLGQWPADLLAKYIGEHMLEHAEADALAQAPPK"
     gene            4499..5131
                     /locus_tag="VFD06_13100"
     CDS             4499..5131
                     /locus_tag="VFD06_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04513.1"
                     /translation="MKTAIANPRAAAIAMAVGFATLLPAFPAPQETRPTPGPDCVECP
                     HWEAPAHLTSRLTIAPPGEPGDRLVLEGTVFRPDGKTPAAGVLLYAYHTNARGYYAHA
                     EGTTGQGRWHGDLRGWLRTGADGRYRIETIRPAPYPSGGIPAHIHMHMMVEGGRERWI
                     DDVVFDDDPLVTAAYRRAAGLRPAGPGIVHLERNAAGVLHGRRDIVLPAE"
BASE COUNT          865 a         1876 c         1758 g          789 t
ORIGIN      
        1 aggggaggag tggcccggcg cgacgatcac ctcgtccggg agcgccagca ggcgatcgag
       61 cagcgaccgc ttcagatccg cgaaatcggt gtgccccgga gcggtcgtgc ttccgatgga
      121 gccgcggaac agcgtgtctc cggtgaaaca gaccgtgtcg tcgactagcc aggcggtctg
      181 cccgtcggtg tgcccgggga tgtgcagggc gcggatccgc atcgaacccg tctccacgtc
      241 aaccccgtcc gcgacttcga gatcgacccc ccccagccgc cgcgcctcga gcgggtgcgc
      301 gaggacgcgg gcgccggtgg cgcgccgcag cgcgtcgcgc tccgcgacgt gatcgccgtg
      361 gtgatgggtc aggaggatgt gcgtgaccgc gagacgatgc cggcgcagga ggtcgagcac
      421 cggcccggcc ggtccgccgg cgtccacgag cacggcctcc ccgtcctccc gcggcgccgc
      481 aatccaggtg ttcgagagcc agccgggatg ctcgacgcgt tccaggatca cggtccgtca
      541 gccggtgaac ttgtacccga ggccgtgcac ggtcagcagg aactgcgggt cggacggacg
      601 gttctcgatc ttcttgcgca gcttggcgac gtgcatgtcc acggtgcggg tcagcggcgc
      661 ctcctcgtac ccccagacct cgtcgaggag tcgatcgcgc tggatcacct cgccgcggtg
      721 gttcacgaag tactgcagga gccgcagctc gcgcgccgac atctcgacga gcttcccctt
      781 cttgcgcagc tccgccttcc tgaagtcgac catcacgtcc ccgaaacggt acgcctcggc
      841 ctgcgcgcct cccccggtgc tgcgccgcag aagcgcctcg acccgcgcca tgagctccat
      901 gaagccgaac ggcttggtga cgtaatcgtc cgcccccagc ttgaggccga gcaccttgtc
      961 gatctcctgg ccgcgggcgg tcagcatgat gatcggcacg ctcgagcctg cgcgccggat
     1021 ctctttgcag acgtcgaggc cgctcatctt gggcagcatc acgtccagga tgacgaggtc
     1081 gggagcgcac tccccggcca ggcgcaagcc gtcggcgccg tcgcgggcca gcgtcacctc
     1141 gtacccctcg tagcggaagc cgtcgttcag cgccgccgcc atcgaatcgt cgtcttcaac
     1201 caccaggacc ttcggcatcc ttcctcccac ctcctagacc gttccggatc gcggcagcgc
     1261 ctcgtccgcc ggcggccgcc gcgccggcag gtgcaggatg aacgtgctcc cgcggcccgg
     1321 ggcgctttcg agcgagaccc gccctccgtg caccgtgacg atatggtgca ccagcgacag
     1381 ccccagcccg ctccctttca cttcgtggac cagcccggtg ctgacccggt ggaagcggtc
     1441 gaagatcttc gcctgttcgt ccagcggcac gccgatgccc cgatcgcgca ccgcgatggt
     1501 cacctcaccg tcgcccgccc cgaggaggac ctcgatcgaa cgggcctccc ccgagaactt
     1561 gacggcgttg tcgaggagat tgtgcaccgc ctgaccaagt gcgtcggcgt cggccaggac
     1621 ctccgggagg gccccctccg gggcctcgag ccgtgcggtg aagccgtggt gcttgaggtg
     1681 gacgtcgaac gactgcagca ccccctcgac cacctgccgc acgtcggtcg cggcgaaagt
     1741 gtacgtcttg cggcccgatt cgatgcgcga gaagtcgagg atgttctcga tgaggcgcga
     1801 caggcggcgg ctctcgcgct cgatgaactc gccgtactcg cgcaccttct ccggcgtctg
     1861 cacgcggccg agccgcagga gctccgcgaa cacccggatc gacgccagcg gcgtgcgcag
     1921 ctcgtgcgac acgttggaga cgaaatcggc cttcatggtc gagagccgca tggcgcggtc
     1981 cgccgcgcgc agcgccagca cgaggctgat catcagcgca ccgccgagca ggagcagcag
     2041 actggcgttg atcgcgaagt tgacgctggc gaaatgctcc ggcgtcgaac gatggctgtg
     2101 caggatgagg gtccagtcgg tgaagacgaa ggggaaacgg ctggtcacca cttcgccggg
     2161 cagggccggg tgctcctccc ccaggatcag gcgtccctgc ccgtccttga tcatcacctc
     2221 gaggtccttc ccggtctccc ccgggaagaa ctccggaagc gacttctgga tgaccgccgg
     2281 caggagcttc ttgcggaaga agccgatgtc gacaatgaac ccggccaccg ccgccacctg
     2341 gtgatcgtcg ctcgtgatcg aattgaggat aacgcggaaa tcggggttgc gttcctccac
     2401 cgtgaggatg cccccctcga ccgggtgctt ctgcatccgg gccatctgcc agggcgtcgt
     2461 cgcggagagg atcgccatgg tctcctcgtc ggcggagact ttgatgaacc gctgcccgcg
     2521 gggatcgagg acgcgcacct cgccagtgtc cagcttggtg aagtcgacga cgaacatccg
     2581 ggacgccccc ttgatcggct tctgcgtcca ctgccccgcg accagatcga gacggtcctg
     2641 tatgaaccag ccggccggga ggttcagcga gcgttccgcg gcggagcggt agaagtactg
     2701 cacctcggtg ccgatcgcct cgagatagtt gtcgagggtg gcgcggcggg cgatggcctg
     2761 catctcgcgc agctgccgca gctgcaccag ctgcaggacg agcaggacga ggagcgggac
     2821 gagcacgccg acgagccccg cccacagcgt gtggcggcgc acgaacgccg cggcccgagt
     2881 cccccagccc ccgtcgctca cgggccggac tataccgcga aggtcgcggc cgatccgccc
     2941 ggagacgggc gcattgacac atcctttaca aacgacacaa ccttttacgc tccccgtcag
     3001 gacttttact tcgcgccgcc gtaatctccc gttcgcaaat ggacagcggg gaggcaagtg
     3061 caatgcaggc gttgaggaac cgacccggca gccgcccgat ggaccccgcg atccagacct
     3121 accctggaac aacccaccct cacagagcga gaaccagagc aatcggcgac aggcggctgc
     3181 cgggcggttc aagactcccg aggaggatcg acacaatgac ccgttccgcg aagtggatcg
     3241 cgctcgtggc tttgacgcta ctcgtgctcg ccccgctggc cgtcgccggc gaggggaagg
     3301 actgcggaga caagagcgcg gccgcggcga aggaaaccca cgcgtccaag aagtgttcct
     3361 acggcaccca ggactgcctc gaccacatgg ccgccaagat gaagaccggc ggctgggtcg
     3421 gcatcgagct cgagaagagc gagaccagcg gcgtgatgac cgtggcgcgc gtcgtcccgg
     3481 gcagcccggc ggagcaggcc ggcttccagg tcggtgacgt cctgttcgcc ctcaacggca
     3541 tcgagatcaa cgagaagaac gagtcggccc tcaaagccgc gaagaaggat tggaagccgg
     3601 gccagaacgt caactacacg ctgaagcgcg gcgggcagtc cgtgaacgcg aacctgacgc
     3661 tcgcggcgat gccggccgac gtcctggcga agttcatcgg cgaccacatg ctcgagcatg
     3721 catcgaccga ggtcgtcgct tcgaagtaat ccctcccgac cccggtcttg tcgggggcgg
     3781 cggcgggcgc cgccgttccc gttgcagccg gagcggggcc gttcggtcgg attccggacc
     3841 accaccgacc gggcggcccc gctctcctgt tatagtttcg acgcgatgtt cccaggggga
     3901 tcgatgatcg ttgttcgcag aatcctggcc ggtgcgggcc tgtcgttgtt gctggcgtgg
     3961 tcgtgcacgc ccgccggcca gcacccgcag ccgggccatg gggcgagagc cgagaagccg
     4021 tcccagccgt ccccctcccc gacgccgaag ccgaacatcg cgcagaaggc ccctgtgccg
     4081 cccaagcaca agtgccccta ctcgacgcag gagtgcctga accacatggc cagccggctc
     4141 caggcgtccg gctggatcgg catcgagtac gagccggagg agggcggcgt catcaccgtg
     4201 cagcgcgtcg tccccggcag cccggccgag aaggccggcc tcactgtcgg tgacgtcctc
     4261 ttttcgatca acggcgtcga gatccggaaa gggaacgatg cggcgatgaa gaaggcgaag
     4321 gtggattatc gccccggcca gaaggtccac tacgtgatca agcgcaatgg cgcttcgaag
     4381 ccgatcgaca tcgtcctcgg acagtggccc gccgacctcc tcgcgaagta catcggcgag
     4441 cacatgctgg agcacgccga ggccgacgcc ctggcgcagg ccccccccaa gtagcaccgt
     4501 gaagacggcg atcgcgaacc cgcgcgcggc ggcgatcgca atggcggtcg ggttcgccac
     4561 gctcctccct gcgtttccgg ccccccagga gacccggccg acgcccggtc ccgactgcgt
     4621 cgagtgcccg cactgggagg cgccggcgca cctgaccagc cgcctcacga tcgccccgcc
     4681 cggcgagccg ggggatcgcc tcgtcctcga aggaacggtg ttccggccgg acgggaagac
     4741 ccccgcggcc ggcgtgctcc tctacgccta tcacaccaac gcccgggggt attacgcgca
     4801 cgccgagggg acgacgggcc aggggcgctg gcacggcgat ctgcgcgggt ggctccggac
     4861 cggcgccgat ggccgctacc ggatcgagac gattcgcccc gccccctacc cgagcggcgg
     4921 aatccccgct cacatccaca tgcacatgat ggtggagggg ggacgcgagc gctggatcga
     4981 cgacgtcgtg ttcgacgacg acccgctggt gaccgcggcc taccgccgcg ccgcaggcct
     5041 ccgacccgca ggtcccggga tcgtccacct cgagcgtaat gccgcggggg tcctgcacgg
     5101 acgccgcgac atcgtcctcc ctgcggagtg agcccggccg ccctcttcgt catcgggctc
     5161 atcgcgttcg ccgcctcggc gctgacgctg ttcgccgggt tcggcctcgg caccctgctc
     5221 ctgccggtct tcgccctgtt cttccccgtt gaggtcgcgg tcgctgcgac cgccgtcgtc
     5281 cacggact
//
LOCUS       DASRIQ010000534         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1776222, whole genome shotgun sequence.
ACCESSION   DASRIQ010000534 DASRIQ010000000
VERSION     DASRIQ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776222"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1281
                     /locus_tag="VFD06_13105"
     CDS             226..1281
                     /locus_tag="VFD06_13105"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HZN04514.1"
                     /translation="MPRSLVRYLRGPYLLARRALVGGLIHRPRRRQLGIGPGPAPLFR
                     TVFLELRTRCNARCAFCAAAVQSETRPDVTMPFEIYAKVIEDLVRLDYRGQIAYHVNN
                     EPLMVPHLERYIEHARERLPRAWIRIYTNGLALSRAKGEALLRAGIDELTVNWYARDP
                     DAPFPPHLLAFRDEVLPRFHSAARVHPGYGPPAPGDRSIFRFNLCHRNVDELLDSRAG
                     SAPNKVCHPGDATGFCEHPFTQLNITVDGRVGRCCADVYFADVMGNVTRQGLLEIWTG
                     GPFEQARRDLLRGDRSGSDLCRRCDFRGVTSQGAPARARAAEWILFRLDRLLRAPAED
                     PAARPAAPGHTPPAVGT"
     gene            1278..1751
                     /locus_tag="VFD06_13110"
     CDS             1278..1751
                     /locus_tag="VFD06_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04515.1"
                     /translation="MRPGLYRVFLPLFLAFYGVGGLLTHSRFGGLDLYPLFAWGLYAD
                     APPSETQYTARLLAIDGRALPEPIELMGTPDFHRAPDYWSDAGVLTRFGAALEAGDAA
                     EAGALRRQFEANVLRDDRVRYEVLKRTYNPLRRRETGAWDEVRLGAFDKDTGAGR"
     gene            1756..>2193
                     /locus_tag="VFD06_13115"
     CDS             1756..>2193
                     /locus_tag="VFD06_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04516.1"
                     /translation="MPISLTHLVDRFWALAPDPEGGLGLEATHLAARTLVRAYYLGQL
                     LIAFQHLALWNHYVGLRSIRPLWPVFWASGDSGRDAMLILLLFAAGSCAAAFFPDRIA
                     ARAAAFFGCLEFSAFVSSFGTVRHVHHLMLLTSFLFVLIPVRSG"
BASE COUNT          273 a          828 c          728 g          364 t
ORIGIN      
        1 atcatcacgc cggccagcgc cggcagcagt cccttgggca tgatcgacct ccgaacgaac
       61 cgatggcggc ctcggcgagg gcgccgggac cgcgacgaac cagtcactgc aggtggaggc
      121 gaggggggga cccgcgccgg acggatagta tacgcaagcc ccgccgcggg ggttggtcct
      181 tgacaccatg gggtttcggg cgtaactgca cgtcaggaac gccctgtgcc tcgatcgctc
      241 gtccgctacc tccgtggtcc gtacctcctg gcgcgccgcg cccttgtggg ggggctcatc
      301 catcgtccgc gccggcggca gctggggatc gggcccggcc ccgccccgct gttccgcacc
      361 gtcttcttgg aactccggac gcgctgcaac gcgcgctgcg cgttctgtgc cgccgccgtg
      421 caatcggaga cccgccccga cgtcaccatg cccttcgaga tctacgcgaa ggtgatcgag
      481 gacctggtcc gcctcgacta ccgcggtcag atcgcctatc acgtcaacaa cgagccgctg
      541 atggtgccgc atctcgagcg gtacatcgag cacgcgcgcg agcgcctgcc acgcgcctgg
      601 atccgcatct acacgaacgg tctggcgctg tcgcgcgcca agggggaagc gctcctgcgc
      661 gccggcatcg acgagctgac ggtgaactgg tacgcgcggg atccggacgc ccccttcccg
      721 ccgcacctcc tggcgttccg cgacgaggtc ctcccgcgct tccactccgc cgcgcgcgtg
      781 cacccgggat acggcccgcc ggcccccggg gaccggtcga tcttccgctt caacctgtgc
      841 caccggaacg tcgacgagtt gctggattcg cgcgccggct ccgccccgaa caaggtctgc
      901 cacccgggtg acgccaccgg gttctgcgag catcccttca cgcagctcaa catcaccgtc
      961 gacggccggg tgggccgctg ctgcgccgat gtctacttcg ccgacgtgat ggggaacgtg
     1021 acccgccagg gcctgctcga gatctggacc ggcgggccgt tcgagcaggc acgtcgcgac
     1081 ctgctgcgcg gcgaccgttc cggcagcgac ctctgccgcc gctgcgattt ccgcggcgtg
     1141 acctcccagg gggcgccggc gcgcgcgcgc gccgccgagt ggatcctgtt ccgcctcgat
     1201 cgcctcctcc gcgctccggc ggaggacccg gcggcgcggc cggcggcgcc cggccacact
     1261 ccgccggcgg tcgggacatg agaccgggcc tctaccgcgt cttcctgccg ctgtttctgg
     1321 cgttctacgg cgttggcggg ctgctcaccc acagccgctt cggcggtctc gacctgtacc
     1381 cgctcttcgc gtgggggctc tacgcggatg ccccccccag cgagacgcag tacaccgccc
     1441 ggctgctggc gatcgacggc cgagcgctcc cggagccgat cgaactgatg gggacgcccg
     1501 acttccatcg cgcccccgac tactggagcg acgccggcgt cctgacgcgc ttcggcgccg
     1561 ccctcgaggc cggcgacgct gcggaagccg gagcgctgcg gcgccagttc gaagccaacg
     1621 tcctgcgcga cgacagggtg cgttacgagg tcctgaagcg gacctacaat ccgctgcgcc
     1681 gccgtgagac gggggcatgg gacgaggtgc ggctgggggc gttcgacaag gacaccggcg
     1741 ccgggcgctg agcggatgcc gatctcgctg acccatctcg tggatcggtt ctgggcgctc
     1801 gccccggatc cggagggcgg cctcggcctc gaggccactc acctcgcggc ccggacgctc
     1861 gtgcgcgctt actatctcgg acagctcctc atcgccttcc agcatctcgc gttgtggaac
     1921 cactatgtcg ggttgcgctc gatccgtccg ctgtggccgg tcttctgggc ctcgggcgac
     1981 tccggccggg acgcgatgct gatcctcctg ctcttcgccg cgggttcttg cgccgcggcc
     2041 ttcttccccg atcgtatcgc cgcccgcgcg gccgcgtttt tcggctgcct ggagttctcc
     2101 gccttcgtct cctcgttcgg gaccgtccgg cacgtgcacc acctgatgct cctgacctcg
     2161 ttcctgttcg tcctgatccc cgtgcggtcc ggc
//
LOCUS       DASRIQ010000535         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3319083, whole genome shotgun sequence.
ACCESSION   DASRIQ010000535 DASRIQ010000000
VERSION     DASRIQ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3319083"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFD06_13120"
     CDS             complement(<1..692)
                     /locus_tag="VFD06_13120"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482903.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HZN04517.1"
                     /translation="MMGRPAAERARRISIWDPAILRPAMIASLRKLDPRVQAKNPVMF
                     IVEAGSLLTTLVFVQEAVTRAGHPVFTGQVAFWLWFTVLFANFAEAMAEGRGKAQADT
                     LRRTRTETMANRMMDGGRTEPVPAASLCKGDRVLVRAGEIIPADGEIVEGVASVDESA
                     ITGESAPVIRESGGDRSGVTGGTRVISDWIHVRVTSDPGHTFLDRMIALVEGAERQKT
                     PNELALSILLAVL"
     gene            complement(689..1303)
                     /gene="kdpC"
                     /locus_tag="VFD06_13125"
     CDS             complement(689..1303)
                     /gene="kdpC"
                     /locus_tag="VFD06_13125"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HZN04518.1"
                     /translation="MIREIRQGILFGLVTMVLFGAGYPLFLWGIGAAVFRAQAEGSLL
                     RRPDGTILGSRLIAQGFRRPEYFQPRPSGVDYNSASAGGTNFGPSNPDLIGSVRERLE
                     TLRARDAADPEAIPSEMLTASGAGLDPDIPPAAARLQAARVAAARGVPPDRVIALVDD
                     RTRPPLLGFLGRPRVNVFELNLALDTAFGAPAEGPQAGKERNES"
     gene            complement(1300..3027)
                     /gene="kdpA"
                     /locus_tag="VFD06_13130"
     CDS             complement(1300..3027)
                     /gene="kdpA"
                     /locus_tag="VFD06_13130"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZN04519.1"
                     /translation="MGLEIVAVLFTILLTIVTSVPLGRYIFRVFSGAKTRLDPLLVPI
                     ERLVLRLTGVDPGRQQTWKQYALSMLVSNVFMWLLTFAIVSLQRFLPLNPDGIGNMEP
                     TLAFNTISSFTTNTNLQHYSGETGLSYFSQLFVIAFLQFVTAATGVAACIATMRALAG
                     DRLKHVGNFYVDLTRASVRLFLPLAVPLAVLLLSQGVPMTFEGAARARTLEGIDQAIA
                     RGVVAPVVAIKQLGTNGGGYFGSNSAHPYENPTPLSNLAEAWAIAVIPMAMVWALGHF
                     AGRRRLASMIFAVMLAIYLPMAGFAVWQETSGNPALAALGVDQATGSMEGKEVRFGAG
                     LSALWAVTTTVTSNGSVNSMHDSMTPLGGLMPLCGMWLNNIFGGVGVGFINMLIFVIV
                     AVFVAGMMIGRTPEFLGKKVEAKEMKLASLALLWHPLAILVGTAIACHVWSVTKDPGT
                     ALGWLKNPGPHGFSEMLYEFTSSAANNGSGFEGLGDNTPFWNIATGMVMLLSRYIPII
                     APLALAGSLAAKPRTPETAGTLGVESYTFGFTLWAVIVILGLLMFMPVAVLGPIAEQL
                     ALRAAGGGA"
BASE COUNT          507 a         1055 c         1035 g          434 t
ORIGIN      
        1 aggacggcga gcaggatgct caaagcgagc tcgttgggcg tcttctgccg ttccgcccct
       61 tcgaccagcg cgatcatccg gtcgaggaag gtgtgcccgg ggtcggaggt gacccggacg
      121 tgaatccagt cggagatgac ccgcgttccc ccggtgaccc ccgaccgatc cccccccgat
      181 tcccggatga ccggcgccga ctccccggtg atggccgatt cgtccaccga cgcgactcct
      241 tcgacgatct cgccgtcggc cgggatgatc tctcccgccc gcaccagcac ccggtccccc
      301 ttgcacaggc tcgctgccgg caccggctcg gtgcggccgc cgtccatcat ccggttggcc
      361 atcgtctcgg tgcgggtgcg gcgaagcgtg tccgcctgcg ccttcccgcg tccctccgcc
      421 atcgcctcgg cgaagttggc gaaaagaacg gtgaaccaca gccagaaggc gacctgcccc
      481 gtgaacaccg ggtgtcccgc ccgggtgacc gcttcctgca cgaagacgag ggtggtcagg
      541 aggctgccgg cctcgacgat gaacatcacc gggttcttcg cctgcacgcg cggatcgagc
      601 ttccggagcg acgcgatcat cgccggtcgg aggatcgcgg gatcccagat ggagatgcgc
      661 cgggcgcgct ccgccgcggg tcgtcccatc atgattcgtt cctctccttc cctgcctgcg
      721 gtccctccgc cggcgccccg aaggcagtgt cgagcgccag attcagctcg aacacgttga
      781 cgcgcggccg gccgaggaac ccgagcagcg gcggccgggt ccgatcgtcg accagggcga
      841 tcacccggtc cggcggaaca ccgcgcgccg ctgccacacg cgccgcctgg agacgcgccg
      901 cggcgggcgg aatgtccgga tcgaggccgg cgccgctcgc cgtcagcatc tcggagggga
      961 tcgcctccgg atccgcggcg tcccgggcgc ggagggtctc gagccgctcg cgcaccgacc
     1021 cgatgagatc cgggttcgac ggaccgaagt tcgtgcctcc ggccgacgcg ctgttgtagt
     1081 cgacgccgga ggggcgcggc tggaagtact ccggacgccg gaatccctgg gcgatgagcc
     1141 gcgagccgag gatcgttccg tcggggcggc gcagcaggct cccctccgcc tgcgcgcgga
     1201 acaccgcggc gccgatgccc cacaggaaca gggggtagcc ggccccgaag aggaccatcg
     1261 tcaccaggcc gaacaggatc ccctgccgga tctcgcggat cacgctcccc ctcccgccgc
     1321 gcgcagcgcc agctgctcgg cgatcggtcc cagcaccgcc accggcatga acatcagcag
     1381 ccccaggatc acgatgaccg cccagagcgt gaatccgaac gtgtagctct cgacgcccag
     1441 cgtgccggcc gtctccggcg tcctcggctt ggccgcgagc gacccggcga gcgccagcgg
     1501 agcgatgatc gggatgtagc gcgacaggag catcaccatc ccggtggcga tgttccagaa
     1561 gggcgtgttg tccccgagcc cttcgaaccc ggagccgttg ttggcggcgg acgaagtgaa
     1621 ctcgtacagc atctcggaga agccgtgcgg cccggggttt ttcagccagc cgagggcggt
     1681 cccggggtcc ttcgtcacgc tccagacgtg gcaggcgatc gcggtgccga cgaggatcgc
     1741 cagcggatgc cagaggaggg cgaggctggc cagcttcatc tccttcgctt cgaccttctt
     1801 gccgaggaac tccggcgtgc gtccgatcat catgccggcg acgaagacgg cgacgatgac
     1861 gaagatcagc atgttgatga agccgacgcc gaccccgccg aagatgttgt tgagccacat
     1921 gccgcacagc ggcatcagtc cgccgagcgg ggtcatcgag tcgtgcatgg aattgaccga
     1981 gccgttggag gtgaccgtcg tcgtcaccgc ccacagcgcg gagagcccgg cgccgaatcg
     2041 gacctccttc ccctccatcg aaccggtcgc ctgatcgacg ccgagcgcgg cgagggcggg
     2101 attgccggag gtctcctgcc agacggcgaa gcccgccatc ggcaggtaga tcgccagcat
     2161 caccgcgaag atcatcgagg ccagacgccg ccggccggcg aagtggccga gcgcccagac
     2221 catcgccatc gggatgacgg cgatcgccca ggcctcggcg aggttcgaaa ggggcgtcgg
     2281 gttctcgtag ggatgcgccg agttcgaccc gaaatagccg ccgccgttcg ttccgagctg
     2341 cttgatggcg acgacgggag cgacgacgcc gcgggcgatc gcctgatcga tcccttcgag
     2401 ggtgcgcgcc cgggcggccc cctcgaaggt catcggcacc ccctgcgaga gcagcaggac
     2461 cgcgagcggc acggccagcg gcaggaacag gcggacgctc gcccgcgtca gatcgacgta
     2521 gaagttgccg acgtgcttca gccgatcccc cgccagcgcc cgcatcgtcg cgatgcaggc
     2581 ggccacgccg gtcgccgcgg tgacgaactg caggaaggcg atgacgaaca gctgggagaa
     2641 atacgacagg ccggtctcgc cgctgtaatg ctggagattc gtgttcgtcg tgaagctgga
     2701 gatcgtgttg aacgcgagcg tcggctccat gttgccgatg ccgtccgggt tgagcggcag
     2761 gaacctctgc agcgagacga tggcgaacgt cagcagccac atgaagacgt tcgacaccag
     2821 catcgacagg gcgtactgct tccaggtctg ctgcctcccc ggatcgaccc ccgtcaggcg
     2881 caggacgaga cgctcgatcg gcacgagcag cgggtcgagc ctcgtcttcg cccccgagaa
     2941 gacacggaag atgtagcggc cgagcggcac gctcgtgacg atggtgagca ggatggtgaa
     3001 caggacggcg acgatctcga gccccatcgg c
//
LOCUS       DASRIQ010000536         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3608599, whole genome shotgun sequence.
ACCESSION   DASRIQ010000536 DASRIQ010000000
VERSION     DASRIQ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608599"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VFD06_13135"
     CDS             complement(<1..1016)
                     /locus_tag="VFD06_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN04520.1"
                     /translation="MTADVTATNGDLTRLGHRLAGEVLGPMKSTFVGKDEIIDLMGVS
                     LVAGENLFLLGPPGTAKSALVFELGRRLEGRIFEYLLTRFTEPNELFGPFDIRKLREG
                     ELLTNTEGMLPEAQLVFLDELLNANSAILNSLLMVLNERIFRRGREKRDLPTLMVIGA
                     SNRLPEDDALGALFDRFLLRVTCDNVPSDRLGDVLHAGWKLDQGMHERGTAVNIDDVR
                     RLHGALSAVELGEVRPHVAELVVRLRHAGLAVSDRRAVKLQRLVAASALLSGRRRALR
                     SDLWVFRYIWDTVEQQEVLAAIVGDAVKAAPQEENEHPRARADAAPDPESLARDLDDL
                     EKRLS"
     gene            complement(1032..1640)
                     /locus_tag="VFD06_13140"
     CDS             complement(1032..1640)
                     /locus_tag="VFD06_13140"
                     /inference="COORDINATES: protein motif:HMM:NF039424.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04521.1"
                     /translation="MVPSALPFVHALLETGRVKVSFDPQVPRELDEAVTELDLAARPH
                     LAFEPPVLSRPAASWALLVTYRACQALVFREIEGEVVRAVLAVPCPEPPSPSVCYSAD
                     LSLRYLPDLLSLAKGIASDDPLVHGLHAICRSWPLSSVGVPDVGELDTGAFLSHPTLR
                     RLYVDRIIERGDGSRLADPAVREAVREAVGDFPNLAPKLQLA"
     gene            1758..>3982
                     /locus_tag="VFD06_13145"
     CDS             1758..>3982
                     /locus_tag="VFD06_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZN04522.1"
                     /translation="MRRHFIQSFLVLTVLACALAARAAEPAPAMAAAGLTADQVHFFE
                     KHIRPLLVENCYQCHSAGAEKGIKGGFSLDTRDGLLKGGDGGAAIVPGSPENSRLIRA
                     IKWTDNKFQMPPKKAMPPEKVGLVEQWVKMGAPDPRTGPATATAPTAVPFEEAKKFWS
                     YQPVQDPPVPAVKNTAWALSPIDHFILQKLEARGLSPNPPADKRALIRRAAYDLTGLP
                     PAPEDVEDFVNDSSPTAFEQAIDKLLASSAYGERWGRHWLDVVRYADTSGCNSDFPVP
                     SLYKYRNYVIDSFNADKPYDRFVREQLAGDLLPAHDAKQERDNLIATGYLALSRRFGS
                     RNNENHLTVEDSIDNIGKAFLGLSVSCARCHDHKFDAIYSSDYYALYGILASTTYAFP
                     GTEIYRHTKDFVPVGTDEDVKEFYEYQSELASLDDKIEELTVERGQVQARMVASDTAA
                     NDPGFTAQRASVEALAAAGVGAQPAALLLQLSSALAQPQYVPGPRTLDQIRAEQLEAR
                     NRQQQLENRPPEIEKIFAATEGKPADARVHRKGEPNNLGDSVPRGFLKVLGGQALHPE
                     AAPLASGRLDLAGWIADNSNPLTARVMVNRIWQHHFGKGIVQTPNDFGVRGKAPTHPE
                     LLDWLANRFMQSGWSVKKVHRLIMLSRAYQISAQDNPANVATDPGNDLLWKFEQRRLS
                     AEEIRDSMLAVGGMLDKEPGGEHPFPPESEWRYTQHRPFVAVYETNKRSVYLMQQRIR
                     K"
BASE COUNT          696 a         1381 c         1339 g          566 t
ORIGIN      
        1 gaaagccgct tctccaggtc gtccaggtcg cgggcgagtg attcggggtc aggcgccgcg
       61 tcggcgcggg cgcgggggtg ctcgttctcc tcctgcggcg cggccttcac cgcgtcgccg
      121 acgatcgccg ccagcacctc ctgctgctcc accgtgtccc agatgtagcg gaacacccag
      181 aggtccgacc gcagcgcccg ccgccggccg gagagcaggg cgctggcggc gacgaggcgc
      241 tggagcttca ccgcccgccg gtcggacacc gccagccccg cgtggcgcag ccggacgacc
      301 agctcggcga cgtgggggcg gacctcgccg agttccacag ccgacagcgc gccgtgaagc
      361 cgcctgacgt catcgatatt cacggccgtc ccgcgctcgt gcatcccctg gtccagtttc
      421 cacccggcgt gcagcacgtc gccgagccgg tcggacggga cgttgtcgca cgtgacgcgc
      481 aggaggaacc ggtcgaacag cgcccccagc gcgtcgtcct ccggcaggcg gttgctggcc
      541 ccgatgacca tgagcgtcgg caagtcgcgc ttctcgcggc cccggcggaa gatccgctcg
      601 ttcagcacca tcagcaggct gttgaggatc gcgctgttgg cgttgagcaa ctcgtcgagg
      661 aacacgagct gcgcctcggg cagcatcccc tccgtgttcg tcagcagctc cccctcgcgg
      721 agcttgcgga tgtcgaaggg gccaaacagc tcgttgggct cggtgaagcg cgtgagcaga
      781 tactcgaaga tccgcccctc cagccgccgg cccagctcga acaccagcgc gctcttggcg
      841 gtgcccggcg ggccgaggag gaataaattc tcccccgcca cgagcgacac gcccatcaag
      901 tcgatgatct cgtccttgcc cacgaaggtg gacttcatgg ggccgagcac ctcgccggcc
      961 aggcgatggc cgaggcgggt caggtctccg ttggtcgcgg tcacgtctgc ggtcacggtt
     1021 ccccttcgag gtcaggccag ctgaagctta ggagcgaggt tgggaaagtc accgacggct
     1081 tcgcggaccg cctcgcggac ggcggggtcg gccaggcggg aaccgtcgcc gcgctcgatg
     1141 atgcggtcga cgtacaaccg tcgcaaggtg gggtgggaca ggaacgcgcc ggtgtccaat
     1201 tcgccgacgt ccgggacgcc gacggaggag agcggccacg atctgcaaat cgcgtgcagc
     1261 ccgtgcacga gcgggtcgtc gctcgcaatg cccttggcca gcgacaggag gtcggggagg
     1321 tagcggagcg aaaggtcggc ggaataacag accgacggcg acggcggctc cgggcacgga
     1381 accgcgagca ccgcccgcac gacttccccc tcgatctccc ggaacaccag cgcctggcac
     1441 gcccggtagg tcacgagcag tgcccaactt gcggcgggac gcgagagaac cggcggttca
     1501 aaagcgaggt gagggcgggc ggcaaggtcg agctccgtca cggcttcgtc aagctcccgc
     1561 ggaacctgcg ggtcgaatga gaccttcacc cggccagtct ccaggagggc gtgaacgaag
     1621 ggaagggcgg acgggaccat tggtcttcgg cttacttgcg caaacgaacc cgcggcggcc
     1681 ggttattgta gggatagcag gacagcttcc cgtagcaacg ttcggccccc gtcggttaga
     1741 atctcagccg caactctatg cgacgccact tcatccagag tttcctcgtc ctgacggtgc
     1801 tggcgtgcgc cctggcggca cgtgcggccg agccggcgcc ggcgatggcc gcggcggggc
     1861 tgacggccga ccaggtgcac ttcttcgaga agcacatccg gccgctcctg gtcgagaact
     1921 gttaccagtg tcacagcgcg ggcgcggaaa aagggatcaa gggcgggttt tcgctggaca
     1981 cgcgggacgg gctcctcaaa ggcggcgacg ggggcgcggc gatcgtgccg gggtcgccgg
     2041 agaacagccg gctgatccgg gcgatcaagt ggactgacaa taagttccag atgccgccga
     2101 agaaggcgat gccgccggag aaagtggggt tggtagagca gtgggtaaag atgggcgcgc
     2161 ccgacccccg caccggcccg gcgaccgcca ccgcgccgac ggccgttccg tttgaggagg
     2221 cgaagaagtt ctggtcgtac cagccggtgc aggatccgcc ggtgccggcc gtgaagaaca
     2281 cggcctgggc gctgtcgccg atcgatcact tcatccttca gaagctcgaa gccagggggc
     2341 tttcgcccaa cccgcccgcc gacaagcgcg ccctcatccg ccgggccgcc tacgacctca
     2401 ccggtctgcc gccggccccg gaggacgtgg aggacttcgt caacgactcc tccccgaccg
     2461 ccttcgagca ggccattgat aagctcctcg cttcgagcgc ctacggcgag cgctgggggc
     2521 ggcactggct cgacgtcgtg cgctacgccg acacctccgg ctgcaactcc gacttccccg
     2581 tcccgtccct ctacaagtac cgcaactacg tcatcgactc cttcaacgcc gacaagccct
     2641 acgaccgctt cgtcagggag cagctcgccg gcgacctgtt gcccgcccac gacgcgaaac
     2701 aggagcgcga caatctcatc gccaccggtt acctcgccct gtcccgccgg ttcggctcgc
     2761 gcaacaacga gaaccacctg acggtcgaag actcgatcga caacatcggc aaggcgttcc
     2821 tcggcctgag cgtcagttgc gcccgctgtc acgaccacaa gttcgacgcc atctacagca
     2881 gcgactacta cgccctctac ggcatcctcg cgagcacgac gtacgccttc cccggcacgg
     2941 agatctaccg gcacaccaag gacttcgtcc ccgtcggcac cgacgaggac gtgaaggagt
     3001 tttacgagta ccagtcggag ctggcgtcgc tggacgacaa gatcgaggag ctgaccgtcg
     3061 agcgcggcca ggtgcaggcg aggatggtcg cctccgacac cgccgccaac gaccccggat
     3121 tcaccgccca gcgcgcctcc gtcgaggcgc tggccgcggc gggcgtcggg gcgcagccag
     3181 cggcgctgtt gctccagctt tcgtccgcgc tggcacagcc gcagtacgtg ccgggaccgc
     3241 gcacgctcga ccagatccgg gccgaacagc tcgaggcgcg gaaccgccag cagcagttgg
     3301 agaaccgccc gccggagatc gagaagatct tcgccgccac cgagggcaag cccgccgacg
     3361 cacgcgtcca ccgcaagggc gagccgaaca acctcggcga tagcgtcccc cgcgggttcc
     3421 tcaaggtgct cggcggccag gccctgcacc cggaggccgc gccgctggcc agcggtcgcc
     3481 tggacctcgc cggctggatc gcggacaaca gcaacccgct gacggcgcgg gtgatggtga
     3541 accggatctg gcagcaccac ttcggcaagg ggatcgtcca gacgcccaac gacttcggcg
     3601 tccgcggcaa ggcgccgacg cacccggaat tgctcgactg gctggccaac cggttcatgc
     3661 agagcggttg gtcagtgaag aaggtgcacc ggctgatcat gctgtcccgc gcttaccaga
     3721 tttcggcgca ggacaacccg gcgaatgtcg cgaccgaccc gggtaacgat ttgctttgga
     3781 agttcgagca gcgccgcctg tcggccgagg agatccgcga ctcgatgctg gccgtcggcg
     3841 gcatgctgga caaggagccc ggcggcgagc accccttccc gccggagtcg gagtggcgct
     3901 acacgcagca ccggccgttc gtggcggtgt acgagacgaa caagcgcagc gtctacctca
     3961 tgcagcagcg gatccgcaag ca
//
LOCUS       DASRIQ010000537         8284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3086374, whole genome shotgun sequence.
ACCESSION   DASRIQ010000537 DASRIQ010000000
VERSION     DASRIQ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3086374"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VFD06_13150"
     CDS             <1..1320
                     /locus_tag="VFD06_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZN04523.1"
                     /translation="KPIRYGYMNGIALTVLTSQLPKLFGFSADGDGPLRKLWAFARGV
                     VDGNANWTAFAVGAGTLAVILLLKGSKRVPGILIAVVGATVVVGVFDLAARAGVSVLG
                     SLPQGLPRFSLPWIGGDDLVQVLIGGGAVALVSFADTSVLSRTYAAKSGVPVDPNQEM
                     VGLGAANLAAGFFQGFPISSSSSRTPVAEAAGARTQLAGVVGAVSVAVLLVAVPDLLQ
                     SLPSTALAAVVIASAIGLIEIRDLRRIWRIQRWECWLSIVCFAGVAVFGAIPGIGLAI
                     LIAVIEFLWDGWRPHSAVLGRAEGVKGYHDITRYPDARRVPGLVLFRWDAPLFFANAE
                     YFQERVMDAVATSPTPVRWLVVAAEPVTSVDVTAADMLAELEQTLHSDGTCLRFAEMK
                     DPVKDKMRRFGLTARFGEATFFPTVGSAVSAYLKANDVDWEDWEDRK"
     gene            complement(1262..1594)
                     /locus_tag="VFD06_13155"
     CDS             complement(1262..1594)
                     /locus_tag="VFD06_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN04524.1"
                     /translation="METLAREVGISRSGLAERFTSLVGLPPMQYLTRWRLQLAGDLLR
                     RDRRTVVAIAGDVGYDSEAAFSRAFKREMGATPAAWRRPPRAPAAPRVTSGPPSPPNP
                     RRWPSGRR"
     gene            complement(1647..3266)
                     /locus_tag="VFD06_13160"
     CDS             complement(1647..3266)
                     /locus_tag="VFD06_13160"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZN04525.1"
                     /translation="MIRRRPLAVIDVGSNSIRSLVVDMDGEGAPKVLDDQREVVRLAS
                     GLRRRGRLSRKAMRRAIEALKRMADIARARGARRVAVVGTSAIREASNRRQFIERVRA
                     ETGLGLRVISEEEEARLAFESAALSFDLGNEPCAVIDLGGGSAELILAFGKHVQEAYS
                     LRLGAVTLTERYLTSDPIRGRELARLQRHVRRRLRAGRIEADPAPRVLIASGGTATTL
                     AQIVMARQGLEGRPVLGFEMTQAELLHLLSAMLRRTLAERRQMPGLSPERADIIVAGA
                     AVLYEIMARLKINLLRVNERGIRHALLHRMIARKASPAKAPATAVPQRVAAAEAFGKT
                     LRFEERHALQVKRIAESLFDQMAKPLDLDPAGRDLLSAAALLHDVGYVVSYRQHHKHA
                     YQLIAHARLEGFTPRERELIALIARYHRRGAPKKRHRPYAVLLKDDRRLVARLASILR
                     LADALDRRHSQRLREVRCRVIDDTVRLVLRSERDLMVEAHAAEEKSDLFGDVFGRDVV
                     VTTRTASKGESATATPPLRLVRKAAGTRTTA"
     gene            complement(3263..4234)
                     /locus_tag="VFD06_13165"
     CDS             complement(3263..4234)
                     /locus_tag="VFD06_13165"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZN04526.1"
                     /translation="MLSHLMMNDGPLTPGRHTKGARAGSGSSRQAAPGPRPRKARRAP
                     PLERLLRQRATTLLRRRKEVKEEGDPDAIHDLRVATRRLQEVLDLFEPVLPREARERA
                     RRRIRRIRHDFAEVRDADVLYGLVRGMRAGSAADEKGAIAPLERRLRLKADRLRRELS
                     GGDHGAPRVAGIRKRLGALLEHLEERPADPDLVARAARIGLARRARELERARRSAASG
                     RPLPAHRLRVAVKRWRYALEILDASSLGPFTEAIEAARRVQEKLGALHDLDVLILLVG
                     RGAGTRSLRTRLAAKRRDLWNEARPLVAAYSDETLRGLRGLPRPRSA"
     gene            4361..5035
                     /gene="tmk"
                     /locus_tag="VFD06_13170"
     CDS             4361..5035
                     /gene="tmk"
                     /locus_tag="VFD06_13170"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZN04527.1"
                     /translation="MSTRRRSSRQRGRLIVVEGIDGSGKSTQIQLLRKWLEARGVKVF
                     FTEWNSSLLVREATKRGKKKNLLTPTTFSLLHATDFADRLTYDIIPYLKAGISVLADR
                     YVYTAFARDVARGVDSRWVQDLYAFATKPDAAFYFRVPIDVAVKRILGARAKIKFYEA
                     GMDLGLSVDPEESFRLFQGRVIEEYDRLTPEFGFKVIDGTRSIDAQQRQVRDIVRRLY
                     DGAAAA"
     gene            5082..5951
                     /locus_tag="VFD06_13175"
     CDS             5082..5951
                     /locus_tag="VFD06_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HZN04528.1"
                     /translation="MKKRFFGNGLPYTDVAGLEGALITIEGTDGVGRSTQVASLKHWL
                     EVQGYGVIETGWTRSELMSETILAAKAGHSLNRLTFSLMYATDFADRLEKIVIPALRS
                     GFVVIADRYVFTAFARSVVRGADPQWIRNVFGFALVPDMTLYLQIDADALLPRILASR
                     KIDFWEAGMDMHLGTDLYESAHKYQWRLIREYNRMARQYGFHSIDATLTPEEIQVRIR
                     RKVQTFLEERRLQTLTENLVQLKPLLFEDEPPVSDESRIAGTARPAPAAGAEPPAPLV
                     DPQRTRTTGPADS"
     gene            5978..6484
                     /gene="sixA"
                     /locus_tag="VFD06_13180"
     CDS             5978..6484
                     /gene="sixA"
                     /locus_tag="VFD06_13180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HZN04529.1"
                     /translation="MLRHAIAAPRDPDRYPDDNLRPLTREGRARMARAARGLKTLEGK
                     FDLILTSPLLRAADTAAILARAFRPRLTIKVLRPLIPGGGAGGVMAGLASLPSDASLV
                     LVGHEPDLSRLAGALVLEQRDTLRVEFKKGGVCRIDFAGVPRLGEGRLIYHLPPRVLR
                     GFRKSGTD"
     gene            complement(6592..6933)
                     /locus_tag="VFD06_13185"
     CDS             complement(6592..6933)
                     /locus_tag="VFD06_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04530.1"
                     /translation="MDSTKDVRNESHNDPQRHQRGQSGQQGRGSQSGRGGSGGHQGGR
                     GGRGGQGGQGGPGGQGDGDRDRQGGQGGDPYRQGGNDPNRPGGSQGGRQGQDNPDLGG
                     QRPGSRHDQDQ"
     gene            complement(7411..7905)
                     /locus_tag="VFD06_13190"
     CDS             complement(7411..7905)
                     /locus_tag="VFD06_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN04531.1"
                     /translation="MAQNVKAIPDGYHTITPALIQREAKKAIDFYRKAFGAEVLASMP
                     SPDGRVMHAEIKIGDSILFVSDEFPEWSPDTKSPQTTGAVTATLNLYVQDADAAFKRA
                     VDAGAKPLMPPSDMFWGDRFGKVVDPFGHHWGLATHKEDLTPDQMEKRKKEWEKQMQQ
                     QKQQ"
     gene            8017..>8284
                     /locus_tag="VFD06_13195"
     CDS             8017..>8284
                     /locus_tag="VFD06_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN04532.1"
                     /translation="MRKATEADRDAILDTLSRAFAEDPVWGGWAFPDRARAMRQRSAV
                     FGLWVDMTLRYGWVKVTEGCEAVASWFPPGTGEDTPEDYDRLVRL"
BASE COUNT         1155 a         2911 c         2946 g         1272 t
ORIGIN      
        1 aagccgatcc gctacggcta catgaacggc atcgcgctga ccgtgctgac gagccagctc
       61 cccaagctct tcgggttctc ggcggacggg gacggccctc ttcgaaaact ctgggccttc
      121 gcccgcgggg tcgtggacgg aaacgcgaac tggacggcgt tcgcggtcgg ggccggcact
      181 ctggcggtga tcctgctcct caaggggagc aagcgggtgc cgggcatcct gatcgctgtg
      241 gtcggggcga ccgtcgtcgt cggcgtcttc gatctggccg cgcgggccgg agtctcggtc
      301 ctgggctcgc tcccgcaggg actgccgaga ttctccctcc cgtggatcgg cggcgacgat
      361 ctggtccagg tgctcatcgg cggcggcgcc gtcgccctcg tgtcgttcgc cgacaccagc
      421 gtgctgtcgc gcacctacgc ggcgaagagc ggcgtcccgg tcgatccgaa ccaggagatg
      481 gtcggtctcg gagccgccaa cctggccgcg ggattcttcc agggcttccc gatcagcagc
      541 agctcgtcgc gcacgccggt cgccgaagcg gcgggagcga gaacgcagct cgccggggtg
      601 gtcggtgccg tctccgtcgc cgtgctcctg gtggcggtgc cggatctcct ccagtccctg
      661 ccttcgacgg ccctggccgc ggtggtgatc gcctcggcca tcggtctgat cgagatccgg
      721 gacctgcgcc gcatctggcg catccagcgc tgggagtgct ggctgtcgat cgtctgcttc
      781 gccggcgtcg cggtgttcgg ggcgatcccg gggatcggcc tggcgatcct gatcgcggtc
      841 atcgagttcc tgtgggacgg ctggcggccg cactccgcgg ttctcgggcg cgccgagggg
      901 gtcaagggct atcacgacat cacccgctat cccgacgccc gccgcgtccc gggactggtg
      961 ctgttccgct gggacgcgcc gctgttcttc gccaacgccg agtacttcca ggagcgggtg
     1021 atggacgcgg tggcgacctc ccccacgccg gtgcggtggc tggtcgtcgc ggcggagccg
     1081 gtcaccagcg tcgatgtcac cgcggccgac atgctggccg agctggaaca gacgctccac
     1141 tccgacggca cctgcctgcg cttcgccgag atgaaggacc cggtcaagga caagatgcgc
     1201 cgcttcggcc tgaccgcgcg cttcggcgag gcgaccttct tcccgaccgt cggttccgcc
     1261 gtcagcgcct acctgaaggc caacgacgtg gattgggagg actgggagga ccggaagtga
     1321 cgcggggcgc agccggggcg cgcggcggcc ggcgccacgc cgccggcgtc gcccccatct
     1381 cacgcttgaa ggcgcggctg aacgccgcct cggagtcgta gccgacgtcc cccgcgatgg
     1441 cgacgaccgt ccgtcggtcc cggcggagca gatcgccggc gagctgcaat cgccaacggg
     1501 tgaggtactg catcggcggc agtcccacga gactcgtgaa ccgctcggcg agccccgagc
     1561 gcgatatccc gacttcgcgg gcgagtgttt ccaccgacca gccctgcgcc ggcttcgagt
     1621 ggatagtggc gggaaaacgg cgggggtcag gcggtggtgc gcgtcccggc ggccttgcgg
     1681 accagccgaa gcgggggggt cgcggtcgcg ctctcccctt tggaggcggt gcgagtcgtc
     1741 accacgacgt cgcggccgaa gacatccccg aacagatccg acttctcctc ggcggcgtgc
     1801 gcctcgacca tgaggtcgcg ctcggagcgc aggacgaggc gcacggtgtc gtcgatgacg
     1861 cggcagcgga cttcgcgcag gcgctgcgag tgccggcgat cgagcgcgtc ggcgagacgc
     1921 aggatcgagg cgaggcgcgc cacgaggcgg cggtcgtctt tcaggagcac cgcgtagggg
     1981 cggtggcgct tcttcggggc gccgcggcgg tggtagcggg cgatgagcgc gatcagctcc
     2041 cgctcgcgcg gcgtgaaccc ttcgagcctg gcgtgcgcga tcagctggta cgcgtgcttg
     2101 tgatgctggc gataggagac gacgtagccg acgtcgtgca gcagcgccgc cgccgacagg
     2161 agatcccggc cggccggatc gagatcgagc ggcttcgcca tctggtcgaa gagcgactcg
     2221 gcgatccgct tcacctggag cgcgtgccgc tcctcgaacc gcagcgtctt gccgaacgcc
     2281 tcggcagcgg cgacgcgctg cggcaccgcg gtggcgggcg ccttcgcggg cgaggccttc
     2341 cgggcgatca tccggtgcag cagggcgtgg cggatgccgc gctcgttgac cctcagaaga
     2401 ttgatcttca gccgcgccat gatctcgtag agcaccgcgg cgccggcgac gatgatgtcg
     2461 gcccgctccg gcgacagccc cggcatctgg cggcgctccg cgagggtgcg gcgcagcatg
     2521 gccgacagca ggtgcaggag ctccgcctgg gtcatctcga agccgaggac cggccggcct
     2581 tcgagaccct gccgcgccat gacgatctgc gccagggtcg tggcggtccc gccggacgcg
     2641 atgaggacgc gcggcgccgg gtccgcctcg atgcgaccgg cgcgcaggcg ccggcggacg
     2701 tggcgctgca gccgggccag ctcgcggccg cggatcggat cactcgtcag gtaccgctcg
     2761 gtgagggtga cggcgccaag tcgcagcgag tacgcctcct ggacgtgctt gccgaaggcc
     2821 aggatcagct cggcgctgcc gccccccagg tcgatgacgg cgcacggctc gttcccgagg
     2881 tcgaacgaca gcgcggcgct ctcgaacgcc aggcgcgcct cctcctcctc ggagatgacc
     2941 cgcaggccga gtccggtctc ggcgcgcacg cgctcgatga actggcgccg gttcgacgcc
     3001 tcgcggatgg cgctcgtgcc gacgaccgcc acgcgccgcg ccccgcgggc ccgcgcgatg
     3061 tcggccatcc gcttgagcgc ctcgatggcg cgtcgcatcg ccttgcgcga cagccggccg
     3121 cggcgcctca gccccgaggc gagccgcacg acctcacgct gatcgtcgag gaccttgggc
     3181 gctccttccc catccatgtc cacgacgagc gagcggatgc tgttcgagcc gacgtcgatg
     3241 acggcgagcg gccggcggcg gatcacgccg acctcgggcg cggcaggccg cgcaggccgc
     3301 ggagcgtctc gtcgctgtac gccgcgacga gcgggcgcgc ctcgttccac agatcgcgcc
     3361 gcttcgcggc gagacgcgtc cgcagcgagc gggtccccgc gccccggccg acgaggagga
     3421 tgaggacgtc gaggtcgtgc agcgccccga gcttctcctg gacgcgccgc gccgcctcga
     3481 tcgcctcggt gaacgggccg aggctcgagg cgtcgaggat ttccagggcg tagcgccagc
     3541 gtttcaccgc gacgcgcagc cggtgcgccg ggagcggccg gcctgaggcg gcgctccgtc
     3601 gcgcgcgctc gagctcgcgg gcgcggcggg cgagcccgat gcgggccgcg cgggcgacga
     3661 gatccggatc ggccgggcgc tcctcgagat gctccagcag cgccccgagc cgtttgcgga
     3721 tgccggcgac gcgcggcgcg ccgtgatccc cgcccgacag ctcccgccgc aggcgatcgg
     3781 ccttgagccg caggcggcgc tcgagcggcg cgatggcccc cttctcatcc gccgcggaac
     3841 cggcccgcat gccgcgcacc aggccgtaga ggacgtcggc gtcccgcact tcggcgaagt
     3901 cgtgccggat gcggcggatc cggcgccgcg cgcgctcacg ggcctcacga ggaaggaccg
     3961 gctcgaacag gtcgagcact tcctgaagcc tgcgcgtcgc cacccgcagg tcgtggatgg
     4021 cgtccgggtc gccttcctct ttgacttcct tgcggcggcg caggagagtc gtcgcccgct
     4081 gccgcagcag acgctcgagc ggcggcgccc gccgggcctt gcggggccgg gggccgggtg
     4141 ccgcctgacg ggacgaaccg ctccccgcac gcgcgccctt ggtgtgcctc ccgggcgtca
     4201 agggaccgtc gttcatcatg agatgggaca gcatcttacg tcggcttcgc gtccaggggg
     4261 atccggtctc gccgatcctt cccgctttcg ccttttcgtc acacgatcgt tgcagaaggg
     4321 gggtggttgg ggcctataat cccgcgcacc ctaaagaggc atgagcactc gcaggcgcag
     4381 ttcgcggcag cgcggccggt tgatcgtcgt cgaagggatc gacggctcgg gcaagagcac
     4441 gcagattcaa ctcctgcgca agtggctcga ggcgcgcggc gtcaaggtct tcttcaccga
     4501 gtggaactcc tccctcctgg tgcgcgaggc gacgaaacgg ggcaagaaga agaacctcct
     4561 gaccccgacc accttcagcc tgctgcacgc gaccgacttc gccgaccggc tgacctacga
     4621 catcatcccg tacctcaagg ccggcatctc cgtgctcgcg gaccgctacg tgtacacggc
     4681 gttcgcgcgc gacgtggcgc gtggcgtcga ttcacgctgg gtgcaggacc tgtacgcctt
     4741 cgcgaccaag ccggacgcgg cgttctactt ccgcgtgccg atcgacgtcg ccgtgaagcg
     4801 catcctcgga gcgcgcgcca agatcaagtt ctacgaggcg gggatggatc tcggtctgtc
     4861 ggtcgatccg gaggaatcgt tccgtctctt ccaggggcgg gtcatcgagg agtacgaccg
     4921 gctcactccc gagttcggct tcaaggtgat cgacggaacc cgcagcatcg acgcgcagca
     4981 gcgccaggtg cgggacatcg tgcgccgtct ctacgacggc gcggcggcgg catgatgtgg
     5041 atccacgggg gtgcgacccg atgactcctt cgaaagcagc gatgaagaag cggttcttcg
     5101 ggaacggcct cccctacacc gacgtcgccg ggctcgaagg ggcgctcatc accatcgagg
     5161 ggaccgacgg cgtcggccgc tcgacccagg tggcgagcct caagcactgg ctcgaggtgc
     5221 agggctacgg cgtcatcgag accggctgga cccgctccga gctgatgtcg gagacgatcc
     5281 tcgcggccaa ggcggggcac agcctgaacc ggctgacctt cagcctcatg tacgccaccg
     5341 acttcgccga ccgtctggag aagatcgtca tcccggcgct gcggtccggg ttcgtggtga
     5401 tcgccgaccg ctacgtcttc accgccttcg cgcgcagcgt cgtgcgcggc gccgatccgc
     5461 agtggatccg gaacgtcttc ggtttcgcgc tcgtgccgga catgacgctc tatctccaga
     5521 tcgacgccga cgcgctcctg ccgcgcatcc tcgcgagccg gaagatcgac ttctgggaag
     5581 ccgggatgga catgcacctc gggaccgatc tctacgagtc ggcgcacaag taccagtggc
     5641 ggctcatccg cgagtacaac cgcatggcgc ggcagtacgg cttccacagc atcgacgcga
     5701 cgctgacgcc ggaggagatc caggtgcgca tccgccgcaa ggtgcagacg ttcctcgagg
     5761 agcggcgcct ccagaccctc accgagaacc tcgtacaact gaagccgctt ctcttcgagg
     5821 acgagccgcc cgtctccgac gaatcccgca tcgccgggac cgcgcgcccc gcgccggccg
     5881 ccggcgccga gccgcccgcg ccgctggtcg atccgcagcg gacgcgcacc accggcccgg
     5941 ccgactcata agaaggacgc gtaccacgcg cctgtacctg ctccgccacg ccatcgcggc
     6001 gccgcgcgac ccggatcgct accccgacga caacctccgg ccgctgacgc gcgaaggacg
     6061 ggcccgcatg gcgcgcgccg cccgcgggct gaagacgctc gaggggaagt tcgacctgat
     6121 cctcacgagc ccgcttttgc gcgccgccga cacggccgcg atcctggcgc gcgcgttccg
     6181 gccgcgcctg acgatcaagg tgttgcggcc gctgatcccc ggcggcggcg cggggggcgt
     6241 gatggccggg ctggcgtcgc tcccgtccga cgcgtcgctc gtgctcgtcg gccacgagcc
     6301 ggatctgtcg cgtctcgccg gagccctcgt gctcgagcag cgcgacaccc tgcgcgtcga
     6361 gttcaagaag ggcggggtct gccggatcga tttcgccggc gtgccgcggc tcggcgaggg
     6421 acgcctgatc taccacctgc cgccgcgcgt cctgcgcggc ttccgcaaga gcggaacgga
     6481 ctgaaagccc gcttcaccag tcgaggctga aagaaaaaca acgccggcgc gcatcaaggg
     6541 ggggtggggg tgtgcgcgcc ggcgcggtga aaaaacgaag ggatcttcgg gctattgatc
     6601 ctggtcgtgg cggctgccgg gacgctggcc gcccaggtcg gggttgtcct ggccctgtcg
     6661 gccgccctga ctgccacccg ggcggttcgg atcgttcccg ccctggcggt acggatcacc
     6721 gccctggccg ccctggcgat cgcggtcgcc atcaccctgg ccacccggac cgccctggcc
     6781 gccctgtccg ccacgaccgc cccggccacc ctggtgtccg cccgagcctc cgcggccgct
     6841 ctggctgccc cgaccctgct ggccgctctg gccacgctga tggcgctgcg gatcgttgtg
     6901 gctctcgttg cgaacatcct tcgtcgaatc cattcgaacc tcctggcgct cgtcgcgccg
     6961 cgtatcgtgc tggcttccgc tgcccctcga atcctcttgc gcacaccttc tacgagggct
     7021 ctcgatgcta gcgtcgggag gaggccgcga ccttccgatc aattactgat gtgggaatca
     7081 cggacacgag aatccgcacg agggcgccca tcctcacgca cgcagtcccg cctcagtcac
     7141 gatcgtgcga gcgtgtggga cgagcatcaa gccggtgggc aggggaagaa agagagtgcg
     7201 ggggtgcgga tcggagggcc ggcaggggcc gcgctcaccg gggcgtgagc gacctcccgc
     7261 gccgggcccc accgcgcggt tcttgcgcag tgggtgcgcg ggaggcgcga tcgccgggag
     7321 gtggcgcgcc gaaggccagc gccaccggac cgggaacccc ggggggcgcg gcccctgccg
     7381 gccccccggt gcggatatcc cggtctcttc ttactgctgc ttctgctgtt gcatctgctt
     7441 ctcccactcc ttcttgcgct tctccatctg gtcgggagtg agatcttcct tgtgcgtcgc
     7501 cagcccccaa tggtggccga agggatcgac gaccttaccg aagcgatcgc cccagaacat
     7561 gtcggagggg ggcatcagcg gcttcgcgcc cgcgtccacc gcccgcttga aggctgcgtc
     7621 ggcgtcctgc acgtagaggt tcagggtggc ggtgaccgcg ccagtcgtct gcggtgactt
     7681 ggtgtcgggg gaccactcgg ggaactcgtc ggagacgaag aggatcgagt ctccgatctt
     7741 gatctcggcg tgcatgacgc ggccgtcagg gctcggcatg ctcgccagga cctcggcgcc
     7801 gaaggccttg cggtagaagt cgatcgcctt cttcgcctcg cgctggatga gggccggggt
     7861 gatggtgtgg tatccgtccg gaatcgcctt gacgttctgt gccattcgaa tgcctcctgg
     7921 cgggcatcgg cccgctcgcg gaaccggatt atgggtgcgt catcatcgta ctcccggtga
     7981 cgcgggcagt cacgcggact tcggtatgct tggacgatgc ggaaggcgac cgaagcggac
     8041 cgcgacgcga tcctcgacac gctctcccgc gcgttcgcgg aggatccggt ctggggcggc
     8101 tgggcctttc cggaccgggc gcgcgcgatg cggcagcgca gcgccgtgtt cggactctgg
     8161 gtcgacatga cgctccgcta cggctgggtg aaggtgaccg agggctgcga ggcggtcgcc
     8221 tcctggttcc cgccggggac cggcgaggac acgcccgagg actacgaccg cctggtgcgg
     8281 ctcg
//
LOCUS       DASRIQ010000538         6363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_983038, whole genome shotgun sequence.
ACCESSION   DASRIQ010000538 DASRIQ010000000
VERSION     DASRIQ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983038"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFD06_13200"
     CDS             <1..221
                     /locus_tag="VFD06_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04533.1"
                     /translation="TIKLRQENQANPKILMIPVALDFGKGTPTIIQKPLLKAEGEIQL
                     RVPEKPKGVTLDPQETQLAFFIDDAKKK"
     gene            405..2633
                     /locus_tag="VFD06_13205"
     CDS             405..2633
                     /locus_tag="VFD06_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HZN04534.1"
                     /translation="MERVFDAFFSVGLDLDRPRTIKDFRLKRDTMELTFSSGTLYLTK
                     PVGGMVTGAYYTGSGTMKATVPNAFDRKLLVDDYGKAVFQESFDQLALRFDDGSEKDI
                     LAASTPGGMPIGGAADAWANRMRIQDNSGSLQVDFLESRINSMPDMAFFTAAIHTPAN
                     EWYSFSHRGRAPIENSIRREKLFGAAGKRWYETVSAFHRPEDYDAKGNYDLPPEADNK
                     MPAAMNHIEMTVTIPNTKSVLIDAMLTAQALRDGVRFARFDFINNTDAASWDEKGRPV
                     TVQAVQDDAGHPLPYLHREHQLLVLLPRPLNRGETARVRVQATEDTIVQLTTSSYWIY
                     SGADWYPRTTSGSEAARHTFDWTVKVAKPMQSVGSGDLKRTWDEGDLSCGQWASTIPV
                     RYASFIFGGFKLAEDRFQSEIAGGASIPLRLYTIHGGGRNFKGKPENVLYNIKEGMRT
                     YESVLGPFPFGDLDIAEMAGGLGFAHSPPGILLISGALDTAGGGGYSDQVIFHELAHQ
                     WWGHQVGRASIEDAWISESWAEYTSELITEAIDKKKAKSMRDEWREGALEVNAYGGTI
                     STAYRSSNAQYRRAWTSLLYDKGPCVLHMLRTWMGWEKFTQYVSTLQSKYRNMNINTD
                     TLAREASQTLGYDMLPFFDQWVRGDGIPKVHYSWTTAPEAGGKQIVTIKLHQEDEANF
                     KVLMLPIGLDFGTGTPTIVPKLVLKAATEIQLRVPQPPRSITMDPSETQLAIFINDDK
                     KK"
     gene            2635..3477
                     /locus_tag="VFD06_13210"
     CDS             2635..3477
                     /locus_tag="VFD06_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN04535.1"
                     /translation="MRTRLLGGLAIAVLFLVLLFAAVRLLEPRLTFLPVGPLQRSPAS
                     IGLPLEDLVAVTADGLRLRGWYLPPPDEAPPDAWTLLVFHGNAENIGNGLDLAQRAHA
                     AGYGVALAEYRGYAGNPGSPSEQGIYRDGEAFFRALSARPGVDPARIAVWGRSIGGAV
                     AVRLAAAGRGGALVLESPFRSVRTLLRDAGAWLFYGLSVLGSYRFDQEGLIGRVEAPI
                     LIVHGTADEVVPFSHGRRLHEAARAPKTFAAIEGGGHNDLWARHAREVWGAAEAFLRH
                     PPGR"
     gene            complement(3546..4298)
                     /locus_tag="VFD06_13215"
     CDS             complement(3546..4298)
                     /locus_tag="VFD06_13215"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN04536.1"
                     /translation="MKSLLVAVGHRLFRWREVLFPTVQLALVFATPPRPWFGGGRGDL
                     LADLAGLALVLAGQGLRAAVIGLAYIRRGGRDRRIDADELVTGGLFAHCRNPLYVGNL
                     LILTGLFVVHGSPWCLVPGILFFAGAYLAITLAEEDYLRGRFGAAYDEYVRRVPRFLP
                     RLQGLRETLAGASFDWPKVIRKEYGATFAWFSLLMVILLREAWQREGAAALHATGLAI
                     AAVWGAGLCGWAAARALKKQGALGGHYRQPAT"
     gene            complement(4315..5100)
                     /gene="cmoA"
                     /locus_tag="VFD06_13220"
     CDS             complement(4315..5100)
                     /gene="cmoA"
                     /locus_tag="VFD06_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HZN04537.1"
                     /translation="MSPTTQTRGATRADRAVPTDRDRLYSETRGRIEDFDFGRDTAAV
                     FDDMLDRSVPQYRVIQEMVAQLACDFARPGTAVVDLGCSTATSIVALDRLLPADLAVR
                     FVGIDSSEEMLAKARAKIAERGVRRPVDLQCADLNGSLPLAGVSVALLVLTLQFVRPL
                     QRERLLRDVRRGLVDGGALILVEKVLGDTPLFNRLWIDHYYRFKESNGYTRLEIAQKR
                     EALENVLIPYRLDENRELLARCGFSQADVFFKWYNFCGVVATT"
     gene            complement(5097..6194)
                     /locus_tag="VFD06_13225"
     CDS             complement(5097..6194)
                     /locus_tag="VFD06_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HZN04538.1"
                     /translation="MSGTMGDLASLRGRIDALNLGLLDLLEERADLVVAIARLKQAQG
                     LEGHDPGREEEMLRALTARATGSFGAFEIGEVFRAIFRASLGVQEKARRDALRVRRRD
                     LHPGGLRVGGVTFGAGRPVMLAGPCAVEDEEQVDRVAAHLAAFPIPTLLRAGAFKPRT
                     NPYSFQGLREEGLRILRRAGRRHGLATVTEVLDVATLPLVAEHADMLQVGARNMYNTE
                     LLKALGRMDKPVLLKRGFSATIEELLLSAEYILAGGNDRVVLCERGIRTFERATRNTL
                     DISAVPILKLETGLPVIVDLSHALGRRDILLPCGRAALAAGADGLMVEVHAAPDRALS
                     DGFQQLDLPSYTALVEGLGIAGTTPRAAAAR"
BASE COUNT         1053 a         2207 c         2198 g          905 t
ORIGIN      
        1 tcaccatcaa gctgcgccag gagaaccagg cgaacccgaa gatcctgatg atcccggtcg
       61 ccctcgactt cggcaagggg acgccgacca tcattcagaa accgctcctg aaggcagagg
      121 gggagatcca gctgcgggtg ccggagaagc cgaagggggt caccctcgac ccgcaggaga
      181 cgcagctcgc cttcttcatc gacgacgcaa agaaaaagta ggagccggcg cggtccgtgg
      241 tgaggagcct catgctgcga ccgcgccgtc tcgggtggat tccagggctc gcgcttctcg
      301 cctcgatccc tagtcccgcg tccgccgagc cccctcagcc gccggctccc ggcgggaccg
      361 aagaggtaat cgtccaggcc tcccccatcg cgactcaggc cgacatggag agagtcttcg
      421 acgccttctt cagtgtcggg ctcgatctcg accgtccgag gacgatcaag gactttcggc
      481 tgaaacgcga caccatggag ctgacctttt cctccggtac gctgtacctg acgaagcctg
      541 tcggcggaat ggtgaccggc gcgtactaca cgggatcggg gaccatgaag gcgaccgttc
      601 cgaacgcctt cgatcgcaag ctcctggtgg acgactacgg gaaggcggtc ttccaggagt
      661 cgttcgatca gctcgccttg agatttgacg atggatccga gaaggacatc ctcgcggcgt
      721 ccacgccggg ggggatgccc atcggggggg cggcggacgc ctgggccaac cggatgcgca
      781 tccaggacaa cagcggtagc ctgcaagtcg atttcctgga atcccgcatc aacagcatgc
      841 ccgacatggc cttcttcacg gcggcgattc acacccccgc gaacgagtgg tattcgttct
      901 cgcaccgggg gcgtgccccg atcgagaact ccatccggcg ggagaagctc ttcggcgccg
      961 ccgggaagag atggtacgag accgtctccg ccttccaccg gccggaggac tacgacgcca
     1021 aaggaaacta cgacctgccg ccggaagccg acaacaagat gcccgcggcc atgaaccaca
     1081 tcgagatgac ggtcacgatc cccaacacca agtcggtgct catcgacgcg atgctgacgg
     1141 cgcaggcgct gcgcgacggg gtccgcttcg cgcgattcga tttcatcaac aacaccgacg
     1201 cggcgtcgtg ggacgagaag ggtcggccgg tcaccgtcca ggccgtgcag gacgacgcgg
     1261 ggcatcctct tccctacctc catcgggagc atcagcttct ggtcctgctc ccccggccgc
     1321 tgaatcgcgg cgagaccgcg cgcgtgcgcg tgcaggcgac cgaggacacc atcgtccagc
     1381 tgacgacctc gtcctactgg atctacagcg gcgccgactg gtacccgcgc acgacgagcg
     1441 ggagcgaggc ggcgcgccac acgttcgact ggacggtgaa ggtcgcgaag ccgatgcagt
     1501 ccgtggggtc cggcgatctg aagcggacgt gggacgaggg ggacctctcg tgcggccagt
     1561 gggcgagcac catcccggtg cgctacgcct cgttcatctt cggaggcttc aagctggccg
     1621 aggatcgctt ccagagcgag atcgccggcg gcgcctcgat tcctctgcgc ctctacacca
     1681 tccacggggg ggggcggaac ttcaagggga agccggagaa cgtgctctac aacatcaagg
     1741 aggggatgag gacctacgag tcggtcctcg gcccgtttcc gttcggagat ctggacatcg
     1801 cggagatggc cgggggactc ggcttcgccc actcccctcc ggggatcctc ctcatcagcg
     1861 gcgcgctcga tacggcgggc ggcgggggat attcggacca ggtgatcttc cacgagctgg
     1921 cccatcaatg gtggggacac caggtcgggc gggcgagcat cgaagacgcc tggatcagcg
     1981 agtcgtgggc cgagtacacg tccgagctga tcaccgaggc gatcgacaag aagaaggcga
     2041 agtcgatgcg cgacgaatgg cgcgaggggg cgctggaggt gaatgcctac gggggaacca
     2101 tcagcaccgc ataccgatcc agcaacgcgc agtaccggcg cgcctggacg tcgctgctgt
     2161 acgacaaggg gccgtgcgtg ctccacatgc tccggacctg gatggggtgg gagaagttca
     2221 cgcagtacgt gtccacgctg cagtcgaagt accgcaacat gaacatcaac accgacacgc
     2281 tggcgcgcga ggcgagccag accctcggct acgacatgct cccgttcttc gaccaatggg
     2341 tgcgcggaga cgggattccc aaagtccatt attcctggac gaccgccccc gaggccggtg
     2401 ggaagcagat cgtcacgatc aagctgcacc aggaggacga ggcgaacttc aaggtcctga
     2461 tgctgccgat cgggctcgat ttcggaaccg gcacccccac catcgtgccg aagctggtcc
     2521 tcaaggcggc gacggagatc cagctgcgcg tgccccaacc ccccaggagc atcacgatgg
     2581 atccgagcga gacgcagctc gccatcttca tcaacgacga caagaagaag taggttgagg
     2641 acccggctcc tgggcggact ggcgatcgcg gtcctgttcc tcgtcctcct tttcgcggcg
     2701 gtgcggctgc tcgagccgcg cctgacgttc ctcccggtgg ggccgctgca gaggtccccc
     2761 gcgtcgatcg ggctgcccct cgaggatctc gtggcggtga cggcggacgg cctgcggctg
     2821 cgcggctggt acctgccgcc ccccgacgaa gcgccgccgg acgcgtggac gctgctcgtc
     2881 ttccacggca acgccgagaa catcggcaat ggcctggatc tggcgcagcg tgcgcacgcg
     2941 gccgggtacg gtgtggccct ggcggagtat cgcggctacg ccgggaaccc gggctccccc
     3001 tcggagcagg ggatctaccg cgacggcgag gcgttcttcc gggcgctctc cgcacggccc
     3061 ggcgtcgacc cggcccgcat cgccgtctgg gggcgctcga tcggcggcgc cgtcgcggtg
     3121 cggctggccg ccgccggccg cggcggtgcg ctcgtgctcg agtcaccgtt ccgttcggtc
     3181 cggacgctcc tgcgcgacgc gggggcgtgg ctgttctatg gcctgtcggt tctcggatcg
     3241 taccgcttcg accaggaagg tctcatcggc cgcgtcgagg cgccgatcct gatcgttcac
     3301 ggcaccgcgg acgaggtcgt cccgttctcg cacgggcgcc ggctccacga ggcggcgcgg
     3361 gcgccgaaaa cgttcgctgc catcgaaggg ggcggacaca acgacctgtg ggcgcggcac
     3421 gcgcgcgagg tctggggtgc cgcggaggcg tttctcaggc acccgcccgg ccgctgagcc
     3481 ccatccgacc caccacgcct gtcgtctctg agccccaagt ccagaacccc ggcccgaccg
     3541 gccggtcagg tcgccggctg gcggtagtgg ccgccgagcg ccccctgctt cttgagggcg
     3601 cgggcggcgg cccagccgca cagccccgcc ccccagacgg cggcgatcgc caaccccgtg
     3661 gcgtgcaacg cggcggcgcc ttccctctgc caggcctcgc gcagcaggat caccatcagc
     3721 agcgagaacc aggcgaaggt ggcgccgtac tccttgcgga tcaccttggg ccagtcgaac
     3781 gacgcccccg cgagcgtctc gcgcaggcct tggagccgcg ggaggaaccg cggcacccgt
     3841 cgcacgtact catcgtaggc ggcgccgaag cggccgcgca ggtagtcctc ctccgccagc
     3901 gtgatcgcca ggtacgcgcc ggcgaagaac aggattccgg ggaccaggca ccacggcgat
     3961 ccgtgcacca cgaacagccc ggtgaggatc aagaggttcc cgacgtagag cgggttgcgg
     4021 cagtgcgcga agagcccgcc ggtgacgagt tcgtcggcgt cgatgcgccg gtcacggccg
     4081 ccgcggcgga tgtaggccag cccgatcacc gccgcccgca acccctgtcc cgcgagcacg
     4141 agcgcgaggc ccgcgagatc ggccaggaga tcgccgcgcc cccctccgaa ccacggtcgc
     4201 ggcggcgtcg cgaaaacgag ggcgagctgc accgtgggga aaaggacctc gcgccagcgg
     4261 aacagccggt gtccgaccgc gacgagaagg cttttcatgg cgtcgccggg cgcctcatgt
     4321 cgtggcgacg acgccgcaga agttgtacca cttgaagaag acgtccgcct gcgagaagcc
     4381 gcaccgcgcc aggagctccc ggttctcgtc cagccggtac gggatcagga cgttctcgag
     4441 cgcctcgcgc ttctgggcga tctcgaggcg ggtgtagccg ttgctctcct tgaagcggta
     4501 gtagtggtcg atccacaggc ggttgaacaa cggggtgtcg ccgagcacct tctccaccag
     4561 gatgagggcg ccgccgtcca cgagtccccg ccgcacgtca cgcagcaggc gctcgcgctg
     4621 cagtggccgc acgaactgca gcgtcaggac gagcagcgcc accgagaccc ccgcgagcgg
     4681 caggctgccg ttgagatcgg cgcactgcaa atcgaccggc cgccgcacgc cgcgctccgc
     4741 gatcttggcg cgcgccttcg cgagcatctc ctcggaggag tcgatgccga cgaatcggac
     4801 cgcgaggtcg gccggcagca ggcgatcgag agcgacgatc gaggtggcgg tggagcagcc
     4861 gagatcgacg accgcggtgc cgggtcgggc gaagtcgcag gccagctgcg cgaccatctc
     4921 ctggatgacg cggtactgcg gcacggaacg atcgagcatg tcgtcgaaca ccgcggcggt
     4981 gtcgcgtccg aagtcgaagt cctcgatgcg gccacgggtc tcggaataga gccggtcgcg
     5041 gtcggttggg acggcccggt ccgcccgcgt cgcgccgcga gtctgcgtcg tcgggctcat
     5101 cgcgccgccg ccgcgcgcgg cgtcgtgccc gcgatcccca gcccttccac gagggcggtg
     5161 taggagggga ggtcgagctg ctggaagccg tcggagaggg cccggtcggg agcggcgtgc
     5221 acctccacca tcaggccgtc ggccccggcg gcgagcgcgg cgcggccgca tggcaggagg
     5281 atgtcgcgcc gtccgagtgc gtggctcagg tcgacgatca ccgggagccc ggtctcgagc
     5341 ttgaggatcg gcaccgccga gatgtcgagc gtgttacggg tggcccgctc gaaggtgcgg
     5401 atgccgcgct cgcacaggac gacccggtcg ttgccgcccg cgaggatgta ctcggccgac
     5461 agcagcagct cctcgatcgt ggcgctgaag ccgcgtttca ggaggaccgg cttgtccatg
     5521 cggccgagcg ccttcaggag ctcggtgttg tacatgttgc gcgccccgac ctgcagcatg
     5581 tcggcgtgct ccgcgaccag cggaagggtc gcgacgtcga gcacctcggt gaccgtcgcg
     5641 aggccgtgcc ggcgcccggc ccggcgcagg atccggagcc cctcctcacg cagcccctgg
     5701 aaggagtaag ggttggtccg cggcttgaac gcgccggcgc gcagaagcgt ggggatcggg
     5761 aacgcggcga gatgcgccgc cacccgatcc acctgctcct cgtcctcgac ggcgcacggc
     5821 ccggcgagca tcaccggccg tcccgctccg aacgtcacgc cgccgacgcg cagcccgccg
     5881 ggatggagat cgcggcggcg cacgcgcagc gcgtcgcgcc gcgccttctc ctgcacaccg
     5941 agcgaggcac ggaagatggc ccggaagacc tcgccgatct cgaaggcgcc gaaggacccc
     6001 gtcgcccgcg ccgtcagggc gcggagcatc tcctcctcgc gccccgggtc gtgcccctcg
     6061 agcccctgcg cctgcttcag ccgggcgatc gccaccacga ggtccgcccg ttcctcgagc
     6121 aggtcgagga ggccgaggtt cagcgcatcg atccgcccgc gcagcgacgc gaggtcgccc
     6181 atggtcccgc tcatgccgac accccccgca tggtcctcac gccgacacga cgcggggccc
     6241 ggtgatctcg atcctcagcc gtccctcgtc cgagaggcgc ggctcgaccc ggtaaccgag
     6301 ggtgacgcag agcgatcgca ccagcgtcag ccccaggccg gcattgtgcc cggcgcgggc
     6361 ctc
//
LOCUS       DASRIQ010000539         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1268403, whole genome shotgun sequence.
ACCESSION   DASRIQ010000539 DASRIQ010000000
VERSION     DASRIQ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268403"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /gene="glgP"
                     /locus_tag="VFD06_13230"
     CDS             complement(<1..1492)
                     /gene="glgP"
                     /locus_tag="VFD06_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZN04539.1"
                     /translation="MSPKTDPFGRLPELARNLWWSWQPEGRALFERLADAAGMPNGPR
                     ALNPLRILPSIAEEQTHALREDAEFMKRLERVLAAFDRMLSAQDAWFPAHAPRHASLP
                     VAYFSAEFGVHQTLPVYSGGLGVLAGDIVKEASDLGLPLVGVGFMYPQGYFNQHIAPD
                     GRQEESYQRLERDFAPAEAVRDKEGKPLVLTLQLPGHLLHVQVSRVEVGRVTLYLMDT
                     DLEQNSPWERELTARLYGGDQETRLLQEILLGIGGVRLVRALGIQPGVWHANEGHAAF
                     MMVERVREKVERGMPFSEAVEAVRLASVFTTHTPVPAGHDAFPFGLVEKYLAAYWPTL
                     GLERDRFLSLGAHQEPWGQAFNMSVLAFRLCGRHSAVSRRHAEVSRRMWSSLWPDRKP
                     EEAPIVAVTNGVHVPSWIAPEMDALFQRHLGEDWGTRQDDPDLWKKVEAIPDDELWET
                     HLSLKRRLHRFLLSRSRRRLLDGRSSPPQVLASGALFDPESLTLGFA"
     gene            complement(1512..1853)
                     /locus_tag="VFD06_13235"
     CDS             complement(1512..1853)
                     /locus_tag="VFD06_13235"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HZN04540.1"
                     /translation="MLEKEKPRKTGGARKKAPAPKTKTAAAPLPPTEKAAALPPSRVV
                     AALGGARATQGSPAVAAGPRTPITAEARWRMIAEAAYFKAQRRGFTGGDAQRDWAEAE
                     AEIDAWLLDRR"
     gene            1994..2692
                     /locus_tag="VFD06_13240"
     CDS             1994..2692
                     /locus_tag="VFD06_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN04541.1"
                     /translation="MSQTILVIDDEKDIVELIRYNLVQAGYRVVSALDGQQALDLARR
                     ERPDLVVLDLMLPTLPGTEVARILRQDEKTRAIPIIMLTARGSEIDRIVGFELGADDY
                     VVKPFSPRELVLRIQAILRREGREESEERIIFEPLVIDLAAHAVRLKGREIDLTATEF
                     KLLHRLARRPGRAFSRDQLLSDVWGYSGDLETRTVDTHMKRLRAKLGAIGTWIETVRG
                     HGYRFRPDGYAAAD"
BASE COUNT          403 a          995 c          957 g          409 t
ORIGIN      
        1 gggcgaatcc gagggtgagc gactccggat cgaacagcgc ccccgacgcc agcacctggg
       61 gcggggagga gcggccgtcg agcagccgcc gccgcgaccg cgacaacagg aaccggtgca
      121 gccggcgctt cagcgagaga tgcgtctccc acagctcgtc gtccgggatc gcctccacct
      181 tcttccagag atccggatcg tcctggcgcg tcccccagtc ctctccgagg tggcgctgga
      241 acagggcgtc catctcgggg gcgatccagg aggggacgtg cacgccgttg gtcaccgcga
      301 cgatcggcgc ttcctccggc ttccggtcgg gccagaggga ggaccacatg cggcgcgaca
      361 cctcggcgtg ccggcggctg acggcgctgt gccgcccgca cagccggaac gccagcacgc
      421 tcatgttgaa cgcctgtccc cacggctcct ggtgcgcccc gagcgagagg aaccggtccc
      481 gttcgaggcc gagggtcggc cagtaggcgg ccaggtactt ctcgacgaga ccgaacggga
      541 aggcgtcgtg ccccgccggc accggcgtgt gggtcgtgaa gacgctggcg aggcgcaccg
      601 cctccaccgc ctcgctgaac ggcatccccc gttcgacctt ctcgcgcacc cgctccacca
      661 tcatgaaggc ggcgtgccct tcgttggcat gccagacgcc gggctggatg ccgagcgccc
      721 gcaccagccg caccccgccg atccccagga ggatctcctg caggaggcgg gtctcctggt
      781 cgccgccgta caggcgcgcc gtcagctccc gctcccacgg cgagttctgt tcgagatcgg
      841 tgtccatcag gtagagcgtg acccgcccca cctcgacccg cgacacctgc acgtgcagca
      901 ggtgccccgg gagctgcagc gtcaggacga gcggcttgcc ttccttgtcg cgcaccgcct
      961 cggccggcgc gaagtcgcgc tcgagcctct ggtacgactc ctcctgccgg ccgtcggggg
     1021 cgatgtgctg gttgaagtac ccctgcgggt acatgaagcc gacccccacg agcggcaggc
     1081 cgagatcgct cgcctccttg acgatgtcgc cggcgagcac gccgagaccg ccggagtaga
     1141 ccgggagcgt ctggtgcacg ccgaactcgg ccgagaagta ggccaccggc agggacgcgt
     1201 ggcgcggcgc gtgcgccggg aaccaggcat cctgcgccga cagcatgcgg tcgaatgccg
     1261 cgagcacccg ctcgaggcgc ttcatgaact cggcgtcttc ccgaagcgcg tgcgtctgct
     1321 cttccgcaat cgaggggagg atgcgcaggg gattgagggc gcgcggaccg ttcggcatcc
     1381 cggcggcatc cgccaggcgc tcgaacaggg cgcgtccttc aggctgccac gaccaccaga
     1441 gattgcgcgc cagctccggg agccggccga acgggtccgt cttcgggctc acgcggcgag
     1501 cggctcggcc gtcaacggcg gtcgagcagc caggcgtcga tctccgcttc cgcctcggcc
     1561 cagtcgcgct gggcgtcgcc gccggtgaag ccgcggcgct gcgccttgaa gtaggccgct
     1621 tcggcgatca tccgccagcg ggcctcggcc gtgatggggg tcctggggcc cgcggccacg
     1681 gccggggatc cctgcgtcgc tcgcgctccg cccagtgcgg ccacgacccg ggagggcggc
     1741 agggcggcgg ccttctccgt gggggggagc ggggcggcgg cggtcttcgt cttcggggcg
     1801 ggggccttct tcctggcgcc gccggtcttc ctcggctttt ccttctcgag cattcctcat
     1861 ctccttcgcg gaagggcaga gtctataacg ccgcggccgt tgtcacccaa tcgtcacggc
     1921 cccgtcacga ggggcactcc gcagcctata attcttgaag ccggggtgat gcgtcacgcc
     1981 cccggagact cacatgtcac agacgatcct cgtgatcgac gacgagaagg acatcgtcga
     2041 gttgatccgc tacaacctgg tgcaggccgg ctatcgcgtc gtgagcgccc tggacggtca
     2101 gcaggcgctc gacctggcgc gccgcgaacg ccccgacctc gtcgtcctcg acctgatgct
     2161 gccgacgctg ccggggaccg aggtcgcgcg catcctgcgt caggacgaga agacccgcgc
     2221 catcccgatc atcatgctca cggcgcgcgg ctcggagatc gatcgcatcg tcggcttcga
     2281 gctgggggcg gatgactacg tcgtcaagcc gttctcgccg cgcgagctcg tcctccggat
     2341 ccaggcgatc ctgcgtcgcg aagggcggga ggagagcgag gagcggatca tcttcgagcc
     2401 gctggtcatc gacctcgccg cccacgcggt gcggctgaag ggacgggaga tcgatctcac
     2461 cgccaccgag ttcaagctcc tgcaccggct ggcgcggcgt cccgggcgcg ccttcagccg
     2521 cgatcagctg ctgagcgacg tgtggggata cagcggcgac ctcgagacgc gcaccgtgga
     2581 cacgcacatg aagcggctgc gggccaagct gggtgcgatc gggacctgga tcgagacggt
     2641 gcgaggccac ggctaccgct tccgcccgga tgggtacgcc gccgccgact gaggcacgaa
     2701 cccactgcga ctccgccccc gcctcaccct ctcgttcatc ctcgtggcgc tcctgtcggc
     2761 gacc
//
LOCUS       DASRIQ010000540         5646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3087227, whole genome shotgun sequence.
ACCESSION   DASRIQ010000540 DASRIQ010000000
VERSION     DASRIQ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3087227"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /locus_tag="VFD06_13245"
     CDS             <1..1715
                     /locus_tag="VFD06_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04542.1"
                     /translation="PMTRLSFMLPSPLALESLDDRQILGLTGDGRTVVFSAREGSVTK
                     LFVRRLGEPAAVPLPGTEGVDDVFLSPDGEWVAFVADGRLRKAPLRGGPVVTLCDAMQ
                     SRGGAWGPDGTIVFAPEVTGGLVRVSSSGGTPTPLTTVDSAAGERSHRWPEFLPDGRS
                     VLFTVGMLDRPGDYDDSAIDIVDLQTGERRNVYQGASMARVTAGGRLLVASRGVVQAL
                     PFDLASGKVRGAPVPVLEGVAGEPSSGVVFFSVSRDGTLAYIPGGVAGRERDVVWVDR
                     AGKTTAIPVPPRQYRDVRISPDGTRLALSEGPGGGRQSDISIHDLAHGTTLRLTADGQ
                     AGTPVWSPDGRFVLYDQYGSLAIVRRPADGSGAPEIISRHQERHALTPASVTPDGAML
                     LLTRGGLASKGDLLSMQLDGSGEAALQTLLATPAHEVEPALSPDGRLFAYTLVEHSDP
                     AIMVQSFPGPGGRWQVSVGRAAVSARWSASGRELFYLTQESLMVVPITSTNPFTYGRP
                     EQVVDLRQIRTAPRWGASQYDVAPDGKRFVFLLDRMREATPQQVNVVLNWLDEVERVA
                     TSGH"
     gene            complement(1746..2804)
                     /locus_tag="VFD06_13250"
     CDS             complement(1746..2804)
                     /locus_tag="VFD06_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZN04543.1"
                     /translation="MSRVRAPRRDDAGAAGSDPGAAIGRGDILYLSRADVEAAGVPMP
                     AIIERLEAAFVEKGHGRTEMPPKPGIHPGPHGNDNFIHAMPALIGAFGAAGMKWVSGF
                     PGNQTKGLPYISGLLILNDAETGLPKAVMDATWITAKRTGAATAVAAKRLAKPGASVV
                     GILGCGVQARTNLEALAVVLPSLAEVRAYDIHPERSRAYAEEAKAIHPKLRFLPVDEP
                     RRAVEGCDVVVTAGPIKRRPEPILEAEWFSEGALGVPLDYDSYWTPAAMRRADRFYTD
                     DTSQLLHTRDGGDYFQTIPDVYADLGEVCAGLKEARKSPRERLLCMNLGLALEDMATA
                     PLVLHQALQRGLGTRLAL"
     gene            complement(2836..3978)
                     /locus_tag="VFD06_13255"
     CDS             complement(2836..3978)
                     /locus_tag="VFD06_13255"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZN04544.1"
                     /translation="MQDVPMAEAIQNLSPAAIAEMWRSAWTTGLIALVAWVTIFLVAH
                     TFVLRLLRRIAARTSWTWDDVVVAAIAWPTRLLILTSGLTVFERILPLGPEWDRACDI
                     MLALAFALAFVLFVDRVVSGFLDRMARTHPAFSGARGLVQGAVHGVVIGIGLLIFLDS
                     VGISIAPILASLGVGSLAVALALQDTLANVFAGFHLIADRPLEAGQFIRLQSGEEGHV
                     VLVGWRSTRLRTQQNNLVVVPNSKLAGTVLTNFDLPTSHMAVHVELVVGMGSDLDRVE
                     RVTLEVAREAQRSAPGGQTDAEPQVRFTGFGDSGLRLTAILNAENLKASNDLKSEFVK
                     RLTDRFRREGIAIPYPTRTLDFPPGTQPAGPQAGFTAGRPGPEARS"
     gene            complement(3994..5274)
                     /locus_tag="VFD06_13260"
     CDS             complement(3994..5274)
                     /locus_tag="VFD06_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HZN04545.1"
                     /translation="MASPKTLTGLDLLARSLPKWLRGRRVGLLMHPASVNARLESARS
                     IVARLCGDRLRALYGPQHGFAGEKQDNMIESGHGRDPVLGIPIHSLYSETRSPTAAML
                     HDIDVLLVDLQDVGTRVYTFEWTTALALEACAAAGKELVVLDRPNPIGGLLLEGNLIR
                     PGYTSFVGLYPVPMRHALTLGELAALVNARLAMRGDGPAASAAGGEEGAPRPRPRKDG
                     PGVRCPGLADLTVIPMKGWRRRMEFDATGLPWVLPSPNMPTLDTAIVYPGQVLLEGTN
                     LSEGRGTTRPFEIFGAPYIDLERVGRRLRRRRLPGVVFRDHSFEPTFHKWKGEVCHGF
                     QIHVTDRRTYRPYLTTLALLQDVIAEHKDAFAWKEPPYEYVTDKPPIDVLTGDPAIRE
                     ALEKGADLAALAQTWRREIESFRRESLAFRLYRD"
BASE COUNT          825 a         1948 c         2039 g          834 t
ORIGIN      
        1 ggccgatgac ccgcctgtcg ttcatgctcc catccccgct cgcactggag tcgctcgacg
       61 accgccagat cctggggttg acgggagatg gccgcacggt ggtcttctcg gcgcgcgaag
      121 ggtcggtcac caaactcttc gtccgtcggc tcggtgagcc ggccgccgtg cccctgccgg
      181 gcaccgaggg ggtggacgac gtgttcctgt cccccgacgg cgagtgggtg gcgttcgtcg
      241 ccgatgggcg gctgcgcaag gccccgctcc gcggcgggcc ggtggtcacg ctgtgcgacg
      301 cgatgcaatc gcggggcggc gcgtggggcc cggacgggac gatcgtcttc gcgccggagg
      361 tcaccggagg gctcgtgcgc gtctcctcct ccggcgggac accgacgcca ttgacgacgg
      421 tcgacagcgc cgcgggggag cgctcgcacc gctggccgga gttcctgccg gacggccgct
      481 ccgtcctgtt cacggtgggg atgctggaca ggccggggga ctacgacgac agcgccatcg
      541 acatcgtcga tctgcagacc ggcgagcggc gtaacgtgta ccagggggcg agcatggcgc
      601 gcgttacggc cggcggacgc ctcctcgtgg cgtcgcgcgg cgtggtccag gcgctgccgt
      661 tcgacctcgc ctcggggaag gtccgcggcg cgccggtccc ggtgctcgaa ggggtggcgg
      721 gggagccgag cagcggggtc gtttttttca gtgtcagccg cgacggcacg ctcgcctaca
      781 tccccggcgg ggtcgcgggg cgcgagcggg acgtggtatg ggtcgatcgc gccggcaaga
      841 cgaccgccat cccggtgccg ccgcgacagt atcgtgacgt gcgcatctcc cccgacggca
      901 cccggctggc cctgagcgag gggccggggg gcgggcggca gagcgacatc tccatccacg
      961 acctcgccca cgggacgacg ttgcgcctga ccgccgacgg ccaggccggg accccggtgt
     1021 ggagtcccga tggccggttc gtcctctacg accagtacgg atccctggcg atcgtgcggc
     1081 gaccggccga cggcagcggt gcacccgaga tcatcagccg gcaccaggag cgccacgccc
     1141 tgacgccggc ttcggtcact ccggacggcg cgatgctcct gctgacccgg ggcggcctgg
     1201 cgagcaaagg ggatctgctg tcgatgcagc tcgacggcag cggggaggcg gcgctgcaga
     1261 cgctcctcgc cactccggcc cacgaggtcg agccggcgct gtccccggac ggccgcctgt
     1321 tcgcctacac gctcgtggaa cattccgacc cggcgatcat ggtgcagtcg ttccccggtc
     1381 ccgggggacg ctggcaggtc tcggtagggc gggcggccgt ctcggcccgc tggtcggcga
     1441 gcggccgcga gctcttctat ctcacccagg aatcgctcat ggtggtgccg atcacgagca
     1501 cgaacccgtt cacctacggc cggccggagc aggtcgtgga cctccgccag atccgcaccg
     1561 caccccgctg gggggccagc cagtacgacg tggcgccgga cgggaagcgc ttcgtcttcc
     1621 tcctcgatcg gatgcgcgag gcgacgccgc aacaggtcaa tgtcgtcctc aactggctcg
     1681 acgaggtgga gcgggtcgca acctccggcc actgacgtcc cgaccggcgg cggaccaacg
     1741 atcggtcaga gcgccaggcg ggtcccgagg ccccgctgca gcgcctggtg gagcacgagc
     1801 ggcgccgtcg ccatgtcctc gagcgcgagg ccgaggttca tgcacaggag gcgctcgcgc
     1861 gggcttttcc tggcctcctt gagtccggcg cacacttcgc cgagatcggc gtacacgtcc
     1921 gggatcgtct ggaagtagtc ccccccgtcg cgcgtgtgca ggagctgcga cgtgtcgtcg
     1981 gtgtagaacc ggtcggcgcg ccgcatcgcg gccggggtcc agtacgagtc gtagtcgagc
     2041 ggaacgccga gcgccccctc ggagaaccac tcggcctcga ggatcggctc ggggcgcctc
     2101 ttgatgggcc cggcggtcac gaccacgtcg catccttcga cggcgcggcg cggctcgtcg
     2161 acaggcagga agcgcagttt cggatggatt gccttcgcct cctcggcgta ggcgcgcgat
     2221 cgctctggat ggatgtcgta ggctcgcacc tcggcgagcg acggcaggac caccgccagc
     2281 gcctcgaggt tggtgcgggc ctgcacgccg cagccgagga tgccgaccac ggacgcgccg
     2341 ggtttcgcga ggcgtttcgc ggcgaccgca gtggcagccc cggtccgctt ggcggtgatc
     2401 caggtggcgt ccatcaccgc cttcggcagc ccggtctctg catcgttgag aatcaagagc
     2461 cccgagatgt acgggagccc cttcgtctgg ttgcccggga acccgctgac ccacttcatg
     2521 ccggcggcgc cgaacgcacc gatgagcgcc ggcatggcgt ggatgaaatt gtcgttgccg
     2581 tgcggccccg gatggatccc cggtttcggc ggcatctcgg tgcggccgtg ccctttctcg
     2641 acgaacgccg cctcgagccg ctcgatgatc gccggcatcg gcacgcccgc cgcttccacg
     2701 tccgctcgcg agagatagag gatgtcgccg cggccgatgg ccgcccctgg gtcggacccg
     2761 gctgcgccgg cgtcgtcgcg tcgcggcgcg cgcacccggc tcaccggtgt gctcctgtgc
     2821 gcaacgtgcg acggctcatg atcgggcctc cggacccggg cggcctgcgg tgaatccggc
     2881 ctgcgggccg gcgggctgcg tgccgggcgg gaaatcgagc gtccgcgtcg ggtaggggat
     2941 ggcgatcccc tcgcgccgga agcgatcggt gaggcgcttg acgaactcgc tcttgaggtc
     3001 gttcgaggct ttgaggttct cggcgttcag gatggccgtg agccggagcc cggaatcccc
     3061 gaagccggtg aagcgcacct gaggctcggc gtcggtctga ccgccggggg cgctccgctg
     3121 cgcctcccgc gccacctcga gggtcacccg ctcgacgcga tcgaggtcgg agcccatccc
     3181 taccaccagc tccacgtgca ccgccatgtg gctcgtgggg agatcgaagt tggtcaggac
     3241 ggtgccggcc agcttggagt tggggacgac gacgaggttg ttctgctgcg tcctgaggcg
     3301 cgtgctgcgc cagcccacga ggacgacgtg cccctcttcg ccgctctgca gccggatgaa
     3361 ctgccctgcc tcgagcggcc ggtcggcgat caggtggaat cccgcgaaca cgttggcgag
     3421 cgtgtcctgg agcgccagcg ccaccgccag cgacccgact ccgagcgacg ccaggatcgg
     3481 cgcgatcgag atgccgacgc tgtcgaggaa gatgaggagc ccgatgccga tgacgacgcc
     3541 gtgcacggcc ccctgcacca gcccgcgcgc tccggagaag gccgggtggg tgcgggccat
     3601 ccgatcgagg aacccgctca ccacgcgatc gacgaacagg acgaaggcga gggcgaacgc
     3661 gagcgccagc atgatgtcgc aggcgcggtc ccactccggc ccgagcggga ggatccgctc
     3721 aaaaacggtc agaccgctcg tcaggatgag cagccgcgtc ggccaggcga tggcggcgac
     3781 caccacatca tcccaggtcc acgaggtgcg cgcggcgatg cgacgcagca ggcgcagcac
     3841 gaaggtgtgc gcgaccagga agatggtcac ccacgcgacg agcgcgatga ggccggtcgt
     3901 ccacgccgag cgccacatct cggcgatcgc ggcggggctc aggttctgta tcgcctcggc
     3961 catcggaacg tcctgcatcc tgcgcctcct cgttcagtcg cggtagagac gaaacgccag
     4021 cgactcgcgg cggaacgact cgatctcccg ccgccaggtc tgcgccagcg ccgccaggtc
     4081 ggcccctttc tcgagcgcct cgcggatcgc cgggtcgccg gtcagcacgt cgatcggcgg
     4141 cttgtcggtg acgtactcgt acggcggctc cttccaggcg aacgcatcct tgtgctcggc
     4201 gatcacgtcc tgcaggagcg ccagcgtcgt caggtacggg cgataggtgc gccggtcggt
     4261 gacgtggatc tggaagccgt ggcacacctc ccccttccac ttgtggaagg tcggctcgaa
     4321 ggagtggtcg cggaagacca cgcccggcag ccggcggcgc cgcagccgcc gtccgacccg
     4381 ctcgagatcg atgtagggcg cgccgaagat ctcgaacggc cgggtggtcc cccgcccctc
     4441 cgagaggttc gtcccttcga gaagcacctg ccccgggtag acgatcgccg tgtcgagcgt
     4501 cggcatgttg ggggagggga ggacccaggg gaggccggtc gcgtcgaact ccatccggcg
     4561 ccgccaccct ttcatcggga tcaccgtgag gtcggcgagc cccggacagc gcacgccggg
     4621 tccgtccttg cgcggccgcg gacggggagc gccttcctcg cctccggccg cggacgccgc
     4681 aggaccgtcc ccccgcatcg cgaggcgggc gttgaccagc gccgccagct cgccgagggt
     4741 cagcgcgtgg cgcatcggca ccgggtagag gcccacgaag gaggtgtagc cggggcggat
     4801 caggttcccc tcgaggagca ggccgccgat cgggttcggc cgatcgagga cgaccagctc
     4861 cttgccggcg gcggcgcagg cctcgagcgc cagcgccgtc gtccactcga aggtgtagac
     4921 gcgcgtcccg acgtcctgga gatcgacgag gagcacgtcg atgtcgtgga gcatcgccgc
     4981 ggtcggcgag cgcgtctcgg agtacaggct gtgaatcggg atgccgagca ccgggtccct
     5041 gccgtgtccc gattcgatca tgttgtcctg tttctcgccg gcgaagccgt gctgcgggcc
     5101 gtacagggcg cgcagccggt cgccgcacag ccgcgccacg atgctgcgcg ccgattcgag
     5161 gcgggcgttc accgacgccg ggtgcatcag caggccgacg cgcctcccgc gcagccactt
     5221 cgggagggat cgcgccagaa gatcgaggcc ggtgagggtt ttcggggacg ccatcgtcgc
     5281 aagccccgcg ccgggaggac ggagcggcgg cggcgggaga ctaccacagg cgaaaaaaaa
     5341 ccgaccgcgg agtgcggtcg gtgaaataga cgaagagaag agaggagatc gggagtctgg
     5401 ggcgagtgcc tcctgctcga actcaggtca agcgtttctc gacgatctcc tctcctgagc
     5461 tcaccgatcc atatacagcg aacagcccgc cgaagcaaga ggtttaattt ccctgcggcg
     5521 caagccccat cgtttcgaga accttgacca cggcttcgag actcggtgtg ctcacgagac
     5581 tcccgcgtgc cgccctgctg atcgcgacga ccacgttcgg atcgaggtcg ccgtggtccg
     5641 gttgta
//
LOCUS       DASRIQ010000541         5293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2834568, whole genome shotgun sequence.
ACCESSION   DASRIQ010000541 DASRIQ010000000
VERSION     DASRIQ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2834568"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..850
                     /gene="rplE"
                     /locus_tag="VFD06_13265"
     CDS             317..850
                     /gene="rplE"
                     /locus_tag="VFD06_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZN04546.1"
                     /translation="MATRYLEKVRPALMEKFGYSSIMQAPRFEKIVLNMGVGDATQDA
                     KLLDAAANELGQITGQRPAIRRAKKSIANFKLREGVAVGCAVTLRGARMWEFYDRFIN
                     VAVPRIRDFRGVNPRSFDGRGNYTVGLTEQIIFPEINLDKVGKIRGMDVTIVTSARTD
                     EEGRELLRLMSMPFRQR"
     gene            900..1085
                     /locus_tag="VFD06_13270"
     CDS             900..1085
                     /locus_tag="VFD06_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZN04547.1"
                     /translation="MAKKSIIEKWKRAPKFKVRAYTRCHRCGRPRGFLRDFGLCRICF
                     RELALAGQIPGVVKSSW"
     gene            1191..1589
                     /gene="rpsH"
                     /locus_tag="VFD06_13275"
     CDS             1191..1589
                     /gene="rpsH"
                     /locus_tag="VFD06_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZN04548.1"
                     /translation="MAVSDPIADFLTCVRNGIRAKHRKVDVNASRVKTELAKVLLRER
                     FINNFKVIEDTKQGVLRIYLKYAGEDTSVITGIRRISTPGRRVYVRKDGIPRVMGGLG
                     TSIVSTSRGLMTDGEAREAGLGGELVCQVW"
     gene            1599..2141
                     /gene="rplF"
                     /locus_tag="VFD06_13280"
     CDS             1599..2141
                     /gene="rplF"
                     /locus_tag="VFD06_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZN04549.1"
                     /translation="MSRMGKRPVEIPGGVTVAVADSTVRVKGPKGELSVPVLAGTTVT
                     VEGSEIRVDAERRTRNPAFGTMRAHIANMVTGVTGGFSKTLEIVGTGYRAAMEGKTLV
                     LQLGYSHPIRFEPPAGITIKVENPTRVVISGADKWQVGQASAEIRAFRPPEPYKGKGI
                     KYEGEYIRRKAGKAAGTASS"
     gene            2138..2503
                     /gene="rplR"
                     /locus_tag="VFD06_13285"
     CDS             2138..2503
                     /gene="rplR"
                     /locus_tag="VFD06_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZN04550.1"
                     /translation="MSGIHDHYERRRIRRYRIRRRVVGSAERPRLSVFRSSKHIYAQV
                     IDDLRGVTLAAASSREDLATRVEGKGKSALSTAVGKLLAERAREKGVEKVCFDRGGYL
                     YHGRVKALADGARAGGLSF"
     gene            2514..3041
                     /gene="rpsE"
                     /locus_tag="VFD06_13290"
     CDS             2514..3041
                     /gene="rpsE"
                     /locus_tag="VFD06_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZN04551.1"
                     /translation="MRGSEAPKSEFIERVINVNRCAKVVKGGRRFSFNAIVAIGDQKG
                     RVGVGLGKANEVADAIRKAGEVAKKSMFQVPVTRSGSIPHQIVGHFGAGEVLLKPATP
                     GTGVIAGAGVRAVLEAAGVQNVLTKCLGSRNPHNLVKATVNGLQRLRRPSDVAAMRGR
                     TLAELLGTAGAQRGA"
     gene            3031..3219
                     /gene="rpmD"
                     /locus_tag="VFD06_13295"
     CDS             3031..3219
                     /gene="rpmD"
                     /locus_tag="VFD06_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZN04552.1"
                     /translation="MAHRIRIRQVKGASGHKADQAATVRALGIRRLHQPVEHNDTPQI
                     RGMIFKIRHLVEVEELNS"
     gene            3234..3692
                     /gene="rplO"
                     /locus_tag="VFD06_13300"
     CDS             3234..3692
                     /gene="rplO"
                     /locus_tag="VFD06_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZN04553.1"
                     /translation="MRIGSIHPAPGSTRPRKRRGKGAATGLGGTSGKGHKGKKARAGG
                     KIPAWFEGGQMPLQRRTPKRGFKNPGRVEYQVVQVGRLDKAPAGATVDRAWLKAAGIV
                     RRPGPIKLLAGGELKTALTIKVDAASESARKLVEAAGGRVELKTADAKGT"
     gene            3692..5011
                     /gene="secY"
                     /locus_tag="VFD06_13305"
     CDS             3692..5011
                     /gene="secY"
                     /locus_tag="VFD06_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZN04554.1"
                     /translation="MLQKLRDIFQVPELKRRILFTLALFVVYRLGEHMPTPGVNAKAL
                     AGAFQSQQGTLFGLYDLFVGGAFSRATVFALGIMPYISSSIILQLLGAVVPYLEKLRK
                     EGEEGQKKLTQLTRYGTVLISIVQSYAYSVFLINMGASQGLTVVPDPGFLFTFSTILT
                     QTTGTIFVMWLGEKITEHGIGNGISLIIFVNIVGGVPNTVAQAYDSFRGGTLSLLTIL
                     AVVVIMVAVIASVVLMTQGMRKIPVQYAKRIVGRRMYGGAATHIPLRINTAGVIPIIF
                     ASSIMILPATVAGFVPPSHPLAAMGQWFNPLGWIYNLIYGVIIVFFTYFYTAVVLNPN
                     DLAENMRKSGGFIPGVRPGRKTAEYIDRVLSRITLPGAIYLAFIATLPDMMIAAMGLP
                     FLGFGGTSVLIVVGVALDTLQQVESHLLLRHYEGFVKRGRIRGRQRM"
     gene            5012..>5293
                     /locus_tag="VFD06_13310"
     CDS             5012..>5293
                     /locus_tag="VFD06_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HZN04555.1"
                     /translation="MRVVLLGPPGVGKGTQGRRLATGHGWALISTGEMLRDAIAKRTP
                     LGIEAGRLVDAGQLVGDEVMVALVKERSGMPDAATGFVMDGYPRTVPQAE"
BASE COUNT          925 a         1664 c         1909 g          795 t
ORIGIN      
        1 agcgcatctc ggtggccagc ggggagacga tcgcgaaggg cgagtgacgg cgccgaagat
       61 gcgccggatc cgtggtcccg aagcaccagg gtcgagggga ccgcggcgat gaagaccctg
      121 gagacgagaa ccaatggcgg acagcaagaa gaagggtgag aagtcggcga aatcgaccgg
      181 aggggccaag ggcgcccgtc cgccggtcgt gcagcccaag ccggccaagg cgtccaaggc
      241 gaagccgacg ggcgaagccg gcgccgcgcc gggtcggccc gagggacgcc cgcacgcacc
      301 cgcgacgaag ccgcgcctgg ccacccgcta cctcgagaag gtgcgccccg cgctgatgga
      361 gaagttcggg tactcgagca tcatgcaggc gcctcgcttc gagaagatcg tcctcaacat
      421 gggcgtgggc gacgccacgc aggacgccaa gctcctggat gccgccgcca acgaactggg
      481 acagatcacg gggcagcggc ccgcgatccg acgcgcgaag aagtcgatcg cgaacttcaa
      541 gctgcgcgag ggtgtcgccg tcggctgcgc ggtcaccctg cgcggggcga ggatgtggga
      601 gttctacgac cgcttcatca atgtcgcggt tccgcgcatt cgcgactttc gcggggtcaa
      661 cccgcggtcg ttcgacgggc gcggcaacta cacggtgggc ctcaccgagc agatcatctt
      721 ccccgagatc aacctcgaca aggtcgggaa gatccggggc atggacgtga ccatcgtcac
      781 gagtgcgagg accgatgagg aagggcgcga gctgctgagg ctcatgagca tgcctttccg
      841 tcagcggtaa tcgggccgtc atcggtagtc gggccggcaa acggtaatcg ggagaacgga
      901 tggccaagaa gtcgatcatc gagaagtgga agcgggctcc gaagttcaag gtgcgcgcgt
      961 acacccgctg ccatcgctgc gggcggccgc gcgggttcct gcgcgacttc ggcttgtgcc
     1021 gcatctgttt ccgcgagctg gcgctcgcag ggcagatccc gggggtggtc aagtccagct
     1081 ggtgagtccg gccgtcgcga gagaagggcc atccggcccc tctcggcgaa gggccaatcg
     1141 aggccaggct gcacgcatcg cgcgtggacg ctggcttcaa gggggcgtga atggcagtca
     1201 gtgacccgat cgcggatttc ctgacctgcg tgcgcaacgg gatccgcgcg aagcatcgca
     1261 aggtggacgt caacgcctcg cgcgtgaaga cggagctcgc caaggtgctc ctgcgagagc
     1321 ggttcatcaa caacttcaag gtgatcgagg acaccaagca gggggtgctc cggatctatc
     1381 tcaagtatgc gggcgaggac acgagcgtga tcaccggcat ccggcggatc agcactcccg
     1441 gtcgccgcgt gtacgtccgc aaggacggga tcccgcgcgt catggggggc ctcggcacct
     1501 cgatcgtctc gacgtcccgt ggcctgatga ccgacggcga ggcccgcgaa gccgggctcg
     1561 gcggcgagct cgtctgccag gtctggtagg gggaacacat gtctcgaatg ggtaagcggc
     1621 cggtcgagat cccgggaggg gtcacggtgg cggtcgccga cagcacggtg cgggtcaagg
     1681 ggcccaaggg cgagttgagc gtcccggtgc tggccggcac gacggtcacg gtcgagggga
     1741 gcgaaatccg cgtcgacgcg gagcgccgca cccgcaatcc ggcattcggc accatgcggg
     1801 ctcacatcgc caacatggtg acgggggtga cgggcgggtt cagcaagacg ctggagatcg
     1861 tcggcaccgg gtaccgggcc gcgatggagg ggaagacgct cgtgctgcag ctcggctact
     1921 cgcatccgat ccgattcgag ccgcccgcgg gcatcacgat caaggtggag aatccgacgc
     1981 gtgtggtcat ctcgggcgcc gacaagtggc aggtgggtca ggcgtccgcc gagatccgcg
     2041 ccttccgtcc gcccgagccg tacaagggca aggggatcaa gtacgagggc gagtacatcc
     2101 ggcgcaaggc cggtaaggcc gccgggacgg cgtcatcatg agcggaatcc atgatcacta
     2161 cgagcgccgg cgcatccggc gctaccgcat tcgccgccgt gtcgtcggat cggcggagcg
     2221 tccgcgtctt tcggtgtttc gcagctcgaa gcacatctat gcgcaggtca tcgacgacct
     2281 gcgcggggtc accctcgccg ccgcgtcgag ccgcgaggat ctcgcgacgc gcgtcgaggg
     2341 caagggcaag tccgcgctca gcacggcggt gggcaagctg ctcgccgagc gggcacgcga
     2401 gaagggcgtc gagaaggtgt gtttcgaccg gggcggctac ctgtaccacg ggcgcgtcaa
     2461 ggcgctcgcc gacggcgctc gcgccggcgg tttgagtttc taggaggttc gaggtgcgag
     2521 gatcggaagc acccaagtcc gagttcatcg agcgcgtgat caacgtgaac cgctgcgcca
     2581 aggtggtgaa gggcggccgg cgcttctcgt tcaacgcgat cgtcgcgatt ggcgaccaga
     2641 aggggcgtgt cggcgtcggt ctcggcaagg ccaacgaggt cgcggacgca atccgcaagg
     2701 ccggcgaagt cgccaagaag agcatgttcc aggtgcccgt gacgcggtcc gggtcgatcc
     2761 cgcaccagat cgtcggccac ttcggggcgg gcgaagtgct gttgaagccc gcgacgcccg
     2821 gcaccggcgt cattgccggc gccggggtgc gtgcggtgct cgaggcggcc ggcgtccaga
     2881 acgtcttgac caagtgcctg ggcagtcgca acccgcacaa cctggtcaag gcgaccgtga
     2941 acggccttca gcgcctgcgc cgcccgtcgg acgtggccgc gatgcgcggc cgaacgctgg
     3001 cggagctgct gggcacggcg ggagcgcagc gtggcgcata ggatccgcat ccggcaggtg
     3061 aagggcgcca gcggccacaa ggccgaccag gccgcgaccg tgcgcgctct cggtatccgc
     3121 cggctccatc agccggtgga gcacaacgat accccccaga tccggggcat gatcttcaag
     3181 atccgccacc tcgtcgaggt ggaggagctc aactcctagg acagggaacg accatgcgta
     3241 tcggcagcat ccatccggcc ccgggctcga cgcgaccgcg caagcgcagg ggcaagggcg
     3301 cggcgaccgg gctcggcggc acgtccggca agggacacaa gggcaagaaa gcacgcgccg
     3361 gcggcaagat ccccgcctgg ttcgaaggcg ggcagatgcc gcttcagcgt cgcacgccca
     3421 agcgcggctt caagaatccg ggccgcgtcg agtaccaggt ggtgcaggtc ggacggctgg
     3481 acaaggctcc ggccggggcg accgtggatc gggcatggct caaggcggcc gggatcgtcc
     3541 ggcggccggg gccgatcaag ctgctggcgg gcggtgagct caagacggcg ctcacgatca
     3601 aggtggacgc cgccagcgaa tccgcacgga agctggtcga agccgcaggc gggcgagtcg
     3661 agctgaagac cgcggacgcc aaggggacct gatgctccag aagctgcgcg acatcttcca
     3721 ggttcccgag ctcaagcgcc gcatcctgtt caccctggcg ctgttcgtgg tctaccgcct
     3781 gggtgagcac atgccgacgc cgggcgtcaa cgccaaggcg ctggcgggcg ccttccagag
     3841 ccagcagggc accttgttcg ggctctacga cctgttcgtg ggcggagcgt tctcgcgcgc
     3901 gacggtgttc gcgctcggca tcatgccgta catctcctcg tccatcatcc tgcagctgct
     3961 cggggccgtg gtcccgtacc tcgagaagct gcgcaaggag ggcgaggaag gtcagaagaa
     4021 gctgacccag ctcacgcggt acggcacggt gctgatctcg atcgtccagt cctatgccta
     4081 cagcgtgttc ctcatcaaca tgggagcctc ccaggggttg acggtggtcc ccgacccggg
     4141 gttcctcttc acgttctcca cgatcctcac gcagacgacc gggacgatct tcgtgatgtg
     4201 gctgggcgag aagatcacgg agcacggaat cggcaacggc atctcgctca tcatcttcgt
     4261 gaacatcgtc ggcggggtgc ccaacacggt ggcgcaggcg tacgacagct tccgcggcgg
     4321 cacgctctcg ctgctcacca tcctggccgt ggtcgtcatc atggtggcgg tcatcgcctc
     4381 cgtggtcctg atgacgcagg ggatgcgcaa gatcccggtg cagtacgcga agcgcatcgt
     4441 gggccgccgc atgtacggag gcgcggccac ccatattccg ctgcggatca acaccgccgg
     4501 ggtgatcccg atcatcttcg catcgagcat catgatcctg cccgccacgg tcgcggggtt
     4561 cgtgccgccg agccatccgc tggcggcgat ggggcagtgg ttcaacccgc tggggtggat
     4621 ctacaacctc atctatggcg tcatcatcgt gttcttcacg tacttctata ccgccgtcgt
     4681 gctcaacccg aacgacctgg cggagaacat gcggaagtcg ggaggattca tcccgggggt
     4741 ccgtccgggg cgcaagaccg cggaatacat cgatcgtgtg ttgtcgcgca tcacgttgcc
     4801 gggggcgatc tatctcgcgt tcatcgccac gctcccggac atgatgatcg cggccatggg
     4861 cctgccgttc ctcggcttcg gcggcaccag cgtcctcatc gtggtggggg tcgcgctcga
     4921 caccctgcag caggtcgagt cgcatctcct gctgcggcat tacgagggat tcgtgaagcg
     4981 cggccgcatc cgtggccgtc agaggatgta ggtgcgggtg gtcctgctgg ggccgcccgg
     5041 ggtcggaaag gggacccagg gtcggcggct cgcgaccgga cacggttggg cgttgatctc
     5101 gaccggagag atgctgcgcg acgcgatcgc gaagcggacg ccgctgggga tcgaggccgg
     5161 gcggctggtg gacgccggac agctggtcgg tgacgaggtg atggtcgcac tggtgaaaga
     5221 gcgcagtgga atgccggacg cggcgacggg cttcgtgatg gatggatacc cgcgcaccgt
     5281 cccgcaggcc gag
//
LOCUS       DASRIQ010000542         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3088560, whole genome shotgun sequence.
ACCESSION   DASRIQ010000542 DASRIQ010000000
VERSION     DASRIQ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3088560"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="rocD"
                     /locus_tag="VFD06_13315"
     CDS             complement(<1..1048)
                     /gene="rocD"
                     /locus_tag="VFD06_13315"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HZN04556.1"
                     /translation="MTPSSKTASASTTTLIEQAEKWSAHNYHPLPVVLVRGEGVWVYD
                     PDGNRYMDMLSAYSALNQGHRHPRIVQAIKDQADKITLTSRAFHNELFGPFCEKLCRL
                     AGMEMALVMNSGAEAVETAVKTARKWGYQTKKVAADKAEIIVCNDNFHGRTTTIVGFS
                     SEPQYKEDFGPFTPGFKLIPYGDLKALEQAITPNTVGFLVEPIQGEAGILIPPAGYLK
                     AALDLCRKNRVLLIADEIQTGFGRTGKMFCADWDGVHPDLYVVGKALGGGFLPVSAVI
                     GKKDTLGLFRPGDHGSTFGGNPLACAVGLAALDVLVDEKLAEKSDEMGRYFMERLRAI
                     NSPRVKEVRGKGLLI"
     gene            1130..>2592
                     /locus_tag="VFD06_13320"
     CDS             1130..>2592
                     /locus_tag="VFD06_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN04557.1"
                     /translation="MYLLASMPLAPGSRIGPYEVLAPLGAGGMGEVYRARDPRLGRDV
                     ALKALPAAMSSDPDRLARLEREAKLLAALNHPNIAAIYGVEEAAGQRVLVLELAAGET
                     LGERLARGTPSLEDTLAISRQIVAALEAAHERGIVHRDLKPDNVKVAPDGGVKVLDFG
                     LAAAFDPIGGASDTGGLMSQSPTMSARMTAAGMLLGTAAYMSPEQARGKPVDKRADIW
                     AFGAVLWEMVTGRRLFDGETVTDLLAAVLRQDVAWETLPATTPPALQRLLRRCLERDP
                     HKRLRDIGEARLVLASVAAGESEGGSPVPVAVGTRPVPRLAWLAGAAALAAATAAAGW
                     FAGVARPLPPRQTTRLSFMLPSPLALESLDDRQIMGLTPDGRTVVFSAREGQVTRLFV
                     RRLGEPAAVPLPGTEGVDDLFLSPDGEWVAFVADGRLRKAPLRGGPVVSLCEAMQSRG
                     GAWGPDGTIVFTPEVTGGLMRVSASGGTPTPLTTVDAT"
BASE COUNT          349 a          895 c          939 g          409 t
ORIGIN      
        1 tgatgaggag ccccttcccg cgcacttcct tgacgcgcgg ggagttgatg gcgcgcaggc
       61 gctccatgaa atagcgcccc atctcgtccg acttctcggc cagcttttca tcgaccagca
      121 catcgagcgc cgcgagaccg acggcgcagg cgagcgggtt gccgccgaac gtcgagccgt
      181 ggtcgccggg gcggaagagg ccgagcgtgt ccttcttccc gatgaccgcc gacaccggca
      241 ggaagccgcc cccgagcgcc ttgccgacga cgtagaggtc cgggtgcacg ccatcccagt
      301 cggcgcagaa catctttccg gtgcggccga acccggtctg gatctcgtcg gcgatcagca
      361 ggacccggtt cttccggcac aggtcgagcg ccgccttgag gtagccggcc ggcgggatca
      421 ggatgccggc ctccccctgg atcggctcga ccaggaagcc gaccgtgttc ggggtgatcg
      481 cctgctcgag cgccttgagg tcgccgtacg ggatgagctt gaagccgggg gtgaacggcc
      541 cgaagtcctc cttgtactgc ggctcggagg agaagccgac gatcgtcgtg gtgcggccgt
      601 ggaaattgtc gttgcagacg atgatctccg ccttgtccgc cgcgaccttc ttcgtctggt
      661 agccccactt gcgcgccgtc ttgacggcgg tctcgaccgc ctcggcgccg ctgttcatca
      721 ccagcgccat ctccatcccg gcgagccggc acagcttctc gcagaacggg ccgaacagct
      781 cgttgtggaa ggcgcgcgac gtcagcgtga tcttgtccgc ctggtccttg atcgcctgca
      841 cgatgcgcgg gtgccggtgc ccctggttga gcgccgagta cgccgacagc atgtccatgt
      901 agcggttccc gtccgggtcg tacacccaca ccccttcgcc gcgcaccagc accaccggga
      961 gcgggtggta gttgtgggcg ctccacttct cggcctgctc gatgagcgtg gtggtggatg
     1021 cggacgcggt cttgctcgac ggggtcatgg attggtctcc ggcggaaagg ccaaggatac
     1081 aacaggagga gcgccgcctc gttccggccg atgaaccggc gcccatctgg tgtaccttct
     1141 ggcctccatg cccctggctc ccggttcgcg tatcggcccg tacgaggtcc tggcccccct
     1201 cggggccggc ggaatggggg aggtgtaccg ggcgcgcgat ccccgcctgg ggcgcgacgt
     1261 cgcgctcaag gcgctgcctg cggccatgtc gtcggatccg gatcgcctcg cccgcctcga
     1321 gcgggaggcg aagctcctgg cggcgctcaa ccatccgaac atcgccgcca tctacggggt
     1381 ggaggaggcg gcgggacagc gggtcctggt cctcgagctg gccgcggggg agacgctggg
     1441 ggagcgcctg gcgcgcggca cgccgtccct cgaggacacg ctcgccatct cgaggcagat
     1501 cgtcgcggcg ctggaagccg cgcacgagcg gggcatcgtc caccgcgatc tcaagccgga
     1561 caacgtgaag gtggcgccgg acggcggcgt gaaggtgctg gatttcggac tggccgccgc
     1621 gttcgacccg atcgggggag catccgatac cggcggcctc atgtcgcagt cgcccaccat
     1681 gtcggcccgc atgaccgcgg ccgggatgct cctcggcacc gccgcgtaca tgagcccgga
     1741 gcaggcgcgc ggcaagccgg tcgacaagcg ggccgacatc tgggccttcg gcgccgtgct
     1801 ctgggagatg gtgaccggga ggcggctctt cgacggagag acggtcaccg atctgctcgc
     1861 cgcggtcctg cgccaggacg ttgcatggga gacgctcccg gccacaacac cgccggcgct
     1921 gcagcggctg ctgcggcgct gcctcgagcg ggatccgcac aaacgcctcc gggatatcgg
     1981 ggaagcccgg ctggtgctgg catcggtcgc ggcgggcgag agtgaaggcg ggtctcccgt
     2041 ccccgtcgct gtgggaacgc gaccggtccc gcgcctcgca tggctggccg gggccgcggc
     2101 gctcgcggcg gccacggcag cggccggctg gttcgccgga gtcgcgcgac cgctcccgcc
     2161 gcgacagacg acgcggctct cgttcatgct cccgtctccg ctcgcactgg agtcgctcga
     2221 cgaccgccag atcatgggcc tgacccccga cgggcggacg gtcgttttct cggcgcggga
     2281 ggggcaggtc acgcggctct tcgtccgccg tctcggcgag ccggcggccg ttccgctgcc
     2341 ggggacggag ggggtggacg atctgttcct gtcccctgat ggagagtggg tggcgttcgt
     2401 cgccgacggg cggctccgga aggcgccgct gcgcggcggg ccggtggtca gcctgtgcga
     2461 agccatgcag tcgcgggggg gcgcctgggg ccccgacggg accatcgtct tcactcccga
     2521 ggtgaccggt gggctcatgc gcgtctccgc gtccggcggg acgccgacac ctctgacgac
     2581 cgtcgacgcc ac
//
LOCUS       DASRIQ010000543        14377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3323493, whole genome shotgun sequence.
ACCESSION   DASRIQ010000543 DASRIQ010000000
VERSION     DASRIQ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 14377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14377
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3323493"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFD06_13325"
     CDS             <1..323
                     /locus_tag="VFD06_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04558.1"
                     /translation="IDPEPFVLERLPERPQPAAASVTVHPASMRPAARPAVSRAVPAM
                     ALETDPFDDNEPIDLEDPLDEPAPVAATIAAGSSREVEVPLSIDVHGRTVQIRLRIQI
                     RLSE"
     gene            323..1156
                     /locus_tag="VFD06_13330"
     CDS             323..1156
                     /locus_tag="VFD06_13330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZN04559.1"
                     /translation="MPSLPGEAAGPSPSPAPSSDLRPAPGRREVLLVLGLVFLLNIVF
                     GTALLMAGLRASIVVTQALAFVLPPLVALRLFYLDGRSVLPFRRPAPRHLLAAILGTL
                     GLNHLLRFYDAWQERVWPPPESLRHLWDGLLAFEGPLDFVWLLAAVAAAPALCEEILF
                     RGFVQSGLVRHSEAAWRGVAATALIFGVFHLDPWRFVVAVLLGLFFGWLRLVSGSLWP
                     AILAHALNNALSISLIALGLAAADRAPGSVLTAAAAAALVLLAIVLGRPRPGAVADRM
                     L"
     gene            1182..2327
                     /locus_tag="VFD06_13335"
     CDS             1182..2327
                     /locus_tag="VFD06_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04560.1"
                     /translation="MTPRRIPLVLLSLMLLAVAAPSRAQEPPAPGVMFTAYPSYGAKF
                     DIASEKFSLVTNMLVYNLSGKTFTDVTFKQTYPEGVTVKETFQRDVANEATGETSSTR
                     KVEGNAFYASMQTWKNRQYVVIFNELEIARRLTNVTFPGVEISYTDAEGQRQSTKLAD
                     NTQDLFIYSNVVGGLTRFLKKYNNIDFDFATATPNRKEWEFAPVVASARGRFPTGVIG
                     TYPAQNNVYTGYFRIRTGPPGDMLQVVVKYDQTEKADAITTEDAALSELRDLLKWCGE
                     FEMVPEALKVTKGKWKKYNDAFVVGGQWRDTIKSRLGGGPMTARVFWGAREDVQYKVI
                     ALAHGRGLMEKSADPNPEKEAALLKELDALIETLKSDIVPSAYDRRH"
     gene            2405..3820
                     /gene="glnA"
                     /locus_tag="VFD06_13340"
     CDS             2405..3820
                     /gene="glnA"
                     /locus_tag="VFD06_13340"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZN04561.1"
                     /translation="MKKPADVLKLAKDQNVRIVDLRFMDLPGMWQHFSVPIGQLSESS
                     FEDGYGFDGSSIRGFQAINESDLLVVPDAETAFLDPFTEIPTLNLICNIFEPITKQRY
                     ARDPRALAQRAEAYLQFTRIADIAYFGPEAEFFIFDDVRFDQKANCGYYFLDSVEGQW
                     NSGREEGPNLGHKPRHKEGYFPVPPSDHFQDLRSEMVINLERCGLTVEAHHHEVATGG
                     QMEIDIRFNTLVRSADNLAIYKYVVKNTARRKGHTVTFMPKPLFGDNGSGMHCHQSLW
                     KGGKNLFAGEGYGGFSEMGLHYIGGLLKHSPALAAIIAPTTNSYKRLTPGFEAPVNLA
                     YSRRNRSAAIRIPMVSANPAAIRLEYRPPDPSANAYVAFAAMLMAGLDGVEKKIDPGK
                     PLDKDIYGLSPQELKNVPSLPGSLDAALSHLEADHDFLLKGDVFTEDMIRLWIDYKRE
                     REVNPMRLRPHPYEFALYYDI"
     gene            3891..6899
                     /locus_tag="VFD06_13345"
     CDS             3891..6899
                     /locus_tag="VFD06_13345"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04562.1"
                     /translation="MSPFRPRVDTAPLFALLPHPLAAGLAPHLERLPDPGEAIALLAR
                     LRERDALPGDPVRLHALLTIGGCSPWLAALLEQDPETIEVLPSLGPAPVAATRDDLEQ
                     SLARFEFRRSGLDLPALLRRFKEREYLRIATADFLRLADLSLTVRSLSRLADILVDRA
                     LRSARALLEARHGEPAARNDRGEIERSGFVVLALGKLGGEELNYSSDIDLLYLFDRDG
                     ETRGTGAGAEGAIANRAYFARLAAEVTRLIGSAGPGGEVFRVDLNLRPGGRDGDLAVS
                     LNAAVAYYRDWAEGWERQALLKARPVAGDLDLGRRFLAAVEPLVYAAQPDPYLALTIG
                     AMKDRIDARLSASGHSERDVKLGRGGIRELEFAVQALQLQRAGRDPWLRQANTLLALH
                     RLATRGLVAVAEYAALGEAYTFLRDVEHRLQLGRNRQTALLPADPGAWTPLARSLFLP
                     ADRRDDEEQAFTAELERHRDAVRSFYDSVIGGAAQRAIGADAPDLLLDRVDDESLVAR
                     LGEAGFADPVSLLKPLQSLRRLLQPARIDPAAAQGLRRATPLLLQAALESKSPRRALA
                     HLEGLFSALVPDPEAFRRFVARPERLAPMVRLLGRSDLFAALLRRQPEILSALEDRGR
                     LVRGPDPEERRAGLEAALASAGKGGGAAALRRWHQAELAAIVVRDLNRQTGLRESLHQ
                     LSDLADRTLDASLALAREETFPDAAAVAPRIAALGLGRLGSREIDYDSDLDLVFLFES
                     RGDNQAEERAAASRLCEAAVRILSTLTRDGQLYEVDLRLRPTGSKGELVATEGALRSY
                     FAHDADVWEMQSFLKARAAAGELDLGRRAALAVEQTVLDRGSSLPAVELVEAVADMRR
                     RQREARGGPPGARRLKHGPGGLSDIDFAIEFLQLRHRVPGPPGKDTRRMLEHLHHLGL
                     LAEEPFAALYEGERFLRHLDHALRLVHGRPMKALPSDPGRLEEAAGLLDRLADPPAGA
                     DLPALFDRRTAAVRAAFEEVLATGGAAPRP"
     gene            complement(6904..8991)
                     /locus_tag="VFD06_13350"
     CDS             complement(6904..8991)
                     /locus_tag="VFD06_13350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN04563.1"
                     /translation="MSLAPSLRVRSRRGPGAARRTLALALLGCGILPGTPAPAAAADP
                     PPSLRWPLDRAKEIVSSFGEYRYDHLHAGLDLSTAGATGLPVRAAGDGEVVRLKVEWR
                     GYGRALYLRLRDGRRIVYGHLERYEEKTLGLESRVERRRRETGNRFPGNIELDPGVKA
                     SRGQVIAWAGESGVGPPHLHVEVRDAQDRPIDPFAAGFARPAHGPAPVFESLTLTAAA
                     PDSLIEGRLRVATFDLRRRDGVFQADRPIVVSGPFDASVSAWDPESGGRAGLAGLEVR
                     VDGAVAYRFEPRRFAFEQGPIAGLLFDHRGSRLGPARYAYRLGRLPGNALADEPGEGG
                     ATGTAPPGAFAPPPGAHRLEVIARSASGDVGRTAVTIRVEPAAASGGATAAGGAAGGG
                     ETARAMQAEVLPRFLDLRLRDGDAAVASDLASLAPGAGGDGIAGWRRLPGGVLSAGLP
                     YEALDGLGPAARARLLEAAGAVSVDCVDRGRGLRREGDRFFVDLPPGARFFTGPLVVR
                     VTAPGGTPEGLVPLSQAVELLPDGESLDVPGRMGFLHGGRPERAGVYRFDAGSGRWGF
                     ESDRVETDAVVAPFRRYGRFALLRDDAAPVLVDVEPAAGRTVGAQPAFAARVTDVGRG
                     LDWNGVRFEVDDRALLSEYDPDRGVAKVIEPPSLTPGRHRLRVVAADRAGNLSAEVER
                     EFSVRAAAAKPAR"
     gene            9107..9949
                     /locus_tag="VFD06_13355"
     CDS             9107..9949
                     /locus_tag="VFD06_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZN04564.1"
                     /translation="MASDPFKNLTIRRKAGETVFTEGDLGSEMYVVQSGEVRLFRVRE
                     GIKQELAVMEKGDFFGELSVLEGLPRTSSAEALTDADLIEINSTTFDRMIRANIEIAV
                     RMLRKLSNRLQEATHKLEAMSQADSSRRPQAPVDLDRPPEQEPPPPAPPLPERPARQT
                     AGKPVPDIAVPDGALGMLILEGGGKVFPIQAAAAVIGRYDPVTGQRPEIDLTQVDINR
                     SVSRRHARLQHQDGAFYLSEEVGALNGTLVNGRRLVPGQPQVLAAGDRIAVGMVALVY
                     KAAS"
     gene            9946..10947
                     /gene="trpS"
                     /locus_tag="VFD06_13360"
     CDS             9946..10947
                     /gene="trpS"
                     /locus_tag="VFD06_13360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZN04565.1"
                     /translation="MTRPRVLSGFRPTGPMHIGHLKGALENWVRLQDTHDCFYAICDW
                     HALTSEYADPSRLPEHIEQMGLDWLAAGLDPERSVIFVQSHVPEHAELHLLLSMIVPL
                     SWLERVPTYKEQQEQITHRDLTNYGFLGYPVLQTADILIYRANFVPVGEDQLAHLELS
                     REIARRFNHLFGEVFPEPQALTTPTPRIPGTDGRKMSKSYGNAISLSDTPEEIRSKIL
                     TMVTDPARKRRSDPGNPDVCPVFDLHKAFSTAATQQEAAAGCRSAGIGCIDCKKMLLG
                     HLKPALWPVQERRRALDRDRGRIHLLLADGARRARDEARRTMERVRQAMHLTPRRPA"
     gene            10944..11777
                     /locus_tag="VFD06_13365"
     CDS             10944..11777
                     /locus_tag="VFD06_13365"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZN04566.1"
                     /translation="MIPEASGSGESAAGLAPIRLDGFQGPIDVLLHLARTGGIDLRGL
                     PLLEVARQCDVWVRTLEAADLEAAGDHLVMAATLLHLKSRMLLPAEPLESAAAEADGE
                     EIPARLAGLVQELRRAAEQLQEREAAMELVYTRPQAVMAEFAGEEGIEADLYALVNAF
                     QGILSRLGAVDRESKRISRESMSLMERVTWLIDLLQRERRVQFRALFEGVGDRLTCIL
                     TFLALLEVLRLRVARAWSSHDREDLVVILNEARPDEADAPVPDAPDPAAAGTPPEESH
                     A"
     gene            11770..12471
                     /gene="scpB"
                     /locus_tag="VFD06_13370"
     CDS             11770..12471
                     /gene="scpB"
                     /locus_tag="VFD06_13370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZN04567.1"
                     /translation="MPETTAPLPDAAKLRSILEALIFAAEEPLTLADLEELFPNAPKE
                     KLQEAIDDVAAQWDGVDRGLQVLRVAGGYRLTTRPDLGEWVRALFRARNRRRLSGAAL
                     ETLAIVAYRQPITTPEIQALRGIDPAGVLQTLLDRRLLKVVGRKKVVGKPMLYGTTPE
                     FLTHFGLNTLADLPTLEEFGALASSLGLPLVHEAPTSTGAFEEEDDEGSGHADEEFED
                     ATESDSERDDDGDDS"
     gene            12422..13189
                     /locus_tag="VFD06_13375"
     CDS             12422..13189
                     /locus_tag="VFD06_13375"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZN04568.1"
                     /translation="MRPKATASATTTGTTPEPSGIRLQKVMAAAGIASRREAERLIQA
                     GRVEVNGRVVTALGTRVDPAGDRIRVDGTAIGGAEHAVTVLLHKPKGVLSTVSDPTGR
                     PTVLDLVRGVRARLFPVGRLDWNSEGLLLLTNDGDLAQRLTHPSNHVPKTYRVKVKGL
                     VGRQALEQVRHGLFLDGRRTRPAPVKRISSQSNTWLEVVLFEGRKNQIRRVFERLGHP
                     VLKLRRTAIGSLVDRDLKPGEWRELSPAEIRALKESR"
     gene            13186..14319
                     /gene="hisC"
                     /locus_tag="VFD06_13380"
     CDS             13186..14319
                     /gene="hisC"
                     /locus_tag="VFD06_13380"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZN04569.1"
                     /translation="MSPRIPDHVLRIAPYVPGKPIEEAQRETGRGDVVKLASNENPLG
                     PSPHALAAIRASAAEAHRYPDGSGYALRSALAVRHNLSPSQVVLGNGSTELVEILAKT
                     FLHDRRNAVVGDPAFIMYRIAVLAMNASLVSVPLRDDRHDLESMAESCTQRTALVYIG
                     NPNNPTGTYVGRAAFERYFARVPAHALTVIDEAYCDYVEAKDYPDGLDFLRAGRHVVV
                     LRTFSKIHGLAGLRIGYGLTSPDVARALEAVRSPFNTSVVAQAAALAALKDRRHVSRS
                     RAGNTRETRFLEAALQKRGLDFVPTSANFILVRTGLGGEELHQRLLREGVIVRPMEAY
                     GYKDAVRVSIGRHEDNERFLRSLDQVLGVRRRRQRSVRRQEPS"
BASE COUNT         2075 a         5320 c         5010 g         1972 t
ORIGIN      
        1 cgatcgatcc tgagccgttc gtgctggagc gcctgccgga gcgcccgcag ccggcggcgg
       61 cgagcgtgac cgtgcacccg gcctcgatgc gccccgcggc caggccggcg gtgtcacgcg
      121 cggtcccggc catggctctg gagaccgatc ccttcgacga caacgagccg atcgatctcg
      181 aggacccgct cgatgagccg gcgcccgtcg cggccacgat cgccgcgggg tcgtcgcgcg
      241 aggtcgaggt gccgctctcc atcgacgtgc acggccggac ggtgcagatc cgtctgagga
      301 tccagatccg cctgtcggag tgatgccgtc gcttcccggg gaggcggccg gcccgtctcc
      361 gtcccccgcg ccgtcgtcgg acctgcgccc ggcgccggga cggcgcgagg tccttctcgt
      421 cctcggactc gtcttcctcc tcaacatcgt gttcggcacg gctcttttga tggccggact
      481 gcgcgccagc atcgtcgtca cgcaggcgct ggcattcgtg ctgccgccgc tcgtcgccct
      541 gcggctcttc tacctggacg ggcggtcggt gctgccgttc cgtcgcccgg ctccccgcca
      601 cctcctggcg gcgatcctcg gcacgctcgg gctgaaccat ctcctgaggt tctacgacgc
      661 atggcaggag cgggtctggc cgccgccgga gtccctgcgc cacctctggg acgggctcct
      721 cgcattcgag gggccgctcg acttcgtgtg gctgctcgcc gcggtggcgg cggcgccggc
      781 gctgtgcgag gagatcctgt tccgcgggtt cgttcagtcg gggctggtgc ggcattcgga
      841 ggccgcctgg cgcggcgtgg ccgcgaccgc gctcatcttc ggcgtcttcc acctcgaccc
      901 ctggcgcttc gtcgtggcgg tgctcctcgg cctgttcttc gggtggctcc ggctggtgag
      961 cggcagcctg tggccggcga tcctggcgca cgccctgaac aacgccctgt cgatttccct
     1021 gatcgccctg gggctggcgg ccgcagaccg cgcgccgggg agcgttctga ccgccgccgc
     1081 ggccgcggcg ctggtgctgc tggcgatcgt cctcggccgc ccgcgccccg gagcagtcgc
     1141 ggaccgcatg ctataatccc gccctttttc cggaggtttc gatgacgccg cgccgcatcc
     1201 ccctggtcct cctctccctg atgctgctcg ccgtcgccgc cccgtcccgg gcgcaggagc
     1261 cgccggcccc gggcgtgatg ttcactgcct acccgtccta cggcgccaag ttcgacatcg
     1321 ccagcgagaa gttctcgctg gtgacgaaca tgctggtcta caacctgagc ggcaagacct
     1381 tcaccgacgt caccttcaag cagacctatc cggagggcgt gacggtgaag gagaccttcc
     1441 agcgcgacgt cgcgaacgag gcgaccggcg agacgtcgtc gacgcgcaag gtggagggga
     1501 acgccttcta cgccagcatg cagacctgga agaaccgcca gtacgtggtg atcttcaacg
     1561 agctggagat cgcccgccgc ctgaccaacg tcaccttccc gggggtggag atctcctaca
     1621 ccgacgcgga gggacagcgg cagtcgacga agctcgccga caacacccag gacctgttca
     1681 tctactcgaa cgtcgtcggg gggctgaccc ggttcctgaa gaagtacaac aacatcgact
     1741 tcgacttcgc cacggcgacg ccgaaccgga aagagtggga gttcgccccg gtcgtcgcca
     1801 gcgcgcgcgg ccggttcccg accggcgtca tcggcaccta cccggctcag aacaacgtct
     1861 acaccgggta cttccgcatc cgcaccggcc cgccggggga catgctccag gtcgtggtca
     1921 agtacgacca gacggagaag gccgacgcca tcaccaccga ggacgccgcc ctgtccgagc
     1981 tgcgcgacct gctcaagtgg tgcggggagt tcgagatggt cccggaggcc ctcaaggtga
     2041 cgaaggggaa gtggaagaag tacaacgacg ccttcgtcgt cggcgggcag tggcgcgaca
     2101 cgatcaagag ccgcctgggt ggcggcccga tgacggcgcg ggtcttctgg ggcgcgcgcg
     2161 aggacgtgca gtacaaggtg atcgccctcg cccacgggcg cgggctcatg gagaagagcg
     2221 ccgacccgaa ccccgagaag gaggcggcgc tcctcaagga gctcgacgcc ctcatcgaga
     2281 ccctgaagtc ggacatcgtg ccgtccgcct acgaccgccg gcactgaccg acgcggccgt
     2341 gcgctacaat gcggtcgccc cgaaagatcc ccatcctttt ttataactgg agacgccgaa
     2401 cgagatgaaa aagcccgccg acgtgttgaa actggcgaag gatcagaacg tccgaatcgt
     2461 cgatctgcgc ttcatggacc tgcccggcat gtggcagcac ttctccgtgc cgatcggaca
     2521 gctgagcgag tcgtccttcg aggacgggta cggcttcgac ggatcgagca tccgcggctt
     2581 ccaggcgatc aacgagagcg acctcctcgt cgtcccggac gccgagaccg cgttcctcga
     2641 cccgttcacc gagatcccca ccctgaacct catctgcaac atcttcgagc cgatcaccaa
     2701 gcagcgctac gcgcgcgacc cgcgggcgtt ggcgcagcgg gccgaggcgt accttcagtt
     2761 cacccgcatc gccgacatcg cctacttcgg tcccgaggcg gagttcttca tcttcgacga
     2821 cgtgcgcttc gaccagaagg cgaactgcgg ctactacttc ctcgactcgg tcgaagggca
     2881 gtggaactcc gggcgcgagg aggggccgaa cctcggccac aagccacgcc acaaggaggg
     2941 gtatttcccg gtcccgccga gcgatcactt ccaggacctg cgctcggaga tggtgatcaa
     3001 cctcgagcgc tgcgggctga ccgtcgaggc gcaccaccac gaggtggcca ccggcgggca
     3061 gatggagatc gacatccgct tcaacaccct cgtccgctcc gccgacaacc tggcgatcta
     3121 caagtacgtc gtgaagaaca cggcgcggcg caaagggcac acggtgacct tcatgccgaa
     3181 accgctgttc ggcgacaacg gcagcggcat gcactgccac cagagcctct ggaagggggg
     3241 gaagaacctc ttcgcggggg aggggtacgg cggcttctcg gagatggggc tgcactacat
     3301 cggcgggctc ctgaagcact cccccgcgct cgcggcgatc atcgcgccga cgaccaactc
     3361 ctacaagcgg ctcacccccg gcttcgaggc gccggtcaac ctggcctact cgcggcgcaa
     3421 ccgctccgcc gccatccgca tcccgatggt ctcggcgaac ccggccgcca tccggctgga
     3481 gtaccggccg ccggatcctt cggccaacgc ctacgtcgct ttcgcggcca tgctcatggc
     3541 ggggctcgac ggcgtcgaga agaagatcga tccgggcaag cccctcgaca aggacatcta
     3601 cgggctcagc ccccaggagc tgaagaacgt gccgtccctc ccgggatcgc tcgacgcggc
     3661 cctctcccac ctcgaagcgg atcacgactt cctgctgaag ggggacgtct tcaccgagga
     3721 catgatccgg ctgtggatcg actacaagcg agagcgcgag gtgaacccga tgcggctgcg
     3781 cccgcacccg tacgagttcg cgctctacta cgacatctga ggcggagcgc cactccggcg
     3841 gcccccgacg accggggccg ccggggacgg cgcgcccgcg gggaccgatc gtgagcccct
     3901 tccgcccccg cgtcgacacc gcaccactct tcgccctcct gccgcacccc ctcgccgcgg
     3961 gactggcgcc gcacctcgag cgcctgcccg atcccggcga ggcgatcgca cttctggcgc
     4021 gcctccgtga gcgcgacgcg ctcccgggcg atccggtgcg gctgcacgcc ctcctgacga
     4081 tcggcggctg cagcccgtgg ctggcggccc tgctcgagca ggacccggag acgatcgagg
     4141 tcctgccctc cctgggtccg gccccggtcg cggcgacgcg cgacgacctg gagcagtcgc
     4201 tcgcccgctt cgagttccgc cgctccgggc tcgacctgcc ggccctgctg cgccgcttca
     4261 aggagcgcga gtacctgcgc atcgcgaccg ccgacttcct gcggctcgcc gatctctccc
     4321 tcacggtgcg ctcgctgtcg cggctcgccg atatcctcgt cgatcgcgcg ctgcggtcgg
     4381 cgcgggcgct cctcgaggcg cggcacggcg agcccgcggc gcgcaacgat cgcggcgaaa
     4441 tcgagcgatc cgggttcgtc gtcctggcgc tcgggaagct gggcggcgaa gagctcaact
     4501 acagcagcga catcgacctc ctgtacctgt tcgaccgcga cggtgagacc cgcggcaccg
     4561 gcgccggcgc ggagggggcg atcgcgaacc gggcgtactt cgcgcgcctg gccgcggagg
     4621 tgacgcgcct catcgggtcg gccggtccgg gcggggaggt gttccgcgtc gatctcaacc
     4681 tccgcccggg cggtcgtgac ggcgacctcg cagtttcgct caacgccgcc gtcgcctact
     4741 accgcgactg ggcggagggt tgggagcgcc aggcgctcct caaggcgcgt ccggtggcgg
     4801 gggacctcga tctcggccgg cgcttcctcg ccgcggtcga gccgctggtg tacgcagcgc
     4861 aacccgatcc gtatctcgcg ctcaccatcg gcgcgatgaa ggaccggatc gacgcccgcc
     4921 tgagcgcctc ggggcattcc gagcgggacg tcaagctggg gcgcggcggc atccgggagc
     4981 tggaattcgc cgtgcaggcg ctgcagctgc agcgggccgg ccgcgatccg tggctgcggc
     5041 aggccaatac gctcctggcg ctgcaccggc tcgccacgcg cggcctcgtc gccgtcgcgg
     5101 agtacgccgc gctgggcgag gcgtacacgt tcctccggga cgtcgagcac cgcctgcagc
     5161 tcggacggaa ccgccagacg gcgctcctgc cggcggatcc cggcgcctgg acgccgctgg
     5221 cgcgatccct gttcctgccg gcggatcggc gcgacgacga ggagcaggcg ttcaccgcgg
     5281 agctcgagcg gcaccgggac gcggtgcggt ccttctacga ctcggtcatc ggcggcgccg
     5341 cgcagcgcgc catcggcgcg gacgcccccg atctgctcct cgatcgcgtc gacgacgagt
     5401 cgctcgtggc gcgcctcggc gaagccggct tcgccgatcc ggtctccctc ctcaagccgc
     5461 tgcagtcgct gcgacgtctg ctgcagccgg cccggatcga tccggccgcg gcgcagggcc
     5521 tccgacgcgc gaccccgctc ctcctgcagg cggcgctcga atcgaagagc ccgcgccgcg
     5581 ccctcgccca tctcgaaggg ctgttctcgg cgctcgtccc cgacccggag gcgttccggc
     5641 ggttcgtcgc gcgccccgag cgtctcgcgc cgatggtgcg cctcctcggc cgcagcgacc
     5701 tgttcgcggc gcttctgcgc cgccagccgg agatcctgtc ggcgctcgaa gatcgcggcc
     5761 gcctcgtgcg tggcccggat cccgaggagc ggcgggcggg cctcgaggcc gcactcgcat
     5821 cggccggcaa ggggggcggg gccgcggccc tgcggcgctg gcaccaggcg gagctcgcgg
     5881 cgatcgtcgt gcgcgacctc aaccgtcaga ccgggctgcg cgagagcctg caccagctct
     5941 ccgatctcgc cgatcggacg ctcgacgcct ccctggcgct ggcgcgcgag gagacgttcc
     6001 cggacgccgc ggcggtggcg ccccgcatcg cggcgctcgg cctgggccgg ctcgggtcgc
     6061 gcgagatcga ctacgactcc gacctcgacc tggtgttcct cttcgagtcc cggggcgaca
     6121 accaggcgga ggagcgggcc gccgcgagcc ggctgtgcga agcagcggtg cggatcctgt
     6181 cgaccctcac ccgcgacggg cagctgtacg aggtcgacct ccgcctgcgc ccgaccggca
     6241 gcaaggggga gctggtggcg accgaggggg cgctgcgctc ctacttcgca cacgacgcgg
     6301 acgtctggga gatgcagtcg ttcctcaagg cgcgcgccgc ggccggggag ctggacctgg
     6361 ggcggcgtgc cgccctcgcg gtggagcaga cggtgctcga tcgcggctcc tcgctccccg
     6421 ccgtggagct ggtggaggcg gtcgccgaca tgcggcggcg ccagcgcgag gcgcgcggcg
     6481 gacctcccgg cgcgcggcgg ctgaagcacg gacccggcgg tctttcggac atcgacttcg
     6541 cgatcgagtt cctgcagctg cggcaccggg tgccgggccc ccccgggaag gacacccggc
     6601 ggatgctgga acacttgcac cacctggggc tcctcgcgga ggagccgttc gcggcgctct
     6661 acgaagggga gcgcttcctg cgccacctgg accacgcgct ccgcctcgtc cacggccggc
     6721 cgatgaaggc gctcccctcc gacccggggc ggctggagga ggcggcgggg ctgctcgacc
     6781 gtctcgcgga tccgccggcg ggggccgatc tcccggccct cttcgatcgg cgcactgccg
     6841 ccgtccgcgc cgcgttcgag gaggtcctgg cgaccggggg ggcggcgccg aggccctagg
     6901 agcctagcgc gccggcttcg ccgcggcggc ccggacggag aactcccgct ccacctcggc
     6961 ggaaaggttt ccggcccggt cggccgccac cacccgcagc cggtgccggc ccggagtcag
     7021 ggaggggggc tcgatcacct tcgcgacgcc gcgatccgga tcgtattcgg agagcagcgc
     7081 ccggtcgtcc acctcgaagc gcacgccgtt ccaatcgaga ccgcggccga cgtcggtgac
     7141 ccgtgccgcg aacgccggct gcgcaccgac ggtgcgtccg gcggccggct ccacgtccac
     7201 gaggaccggc gccgcgtcgt cgcggagcag cgcgaaccgt ccatagcgcc ggaacggcgc
     7261 cacgacggcg tccgtctcca cgcggtccga ctcgaacccc caccgcccgc tccccgcgtc
     7321 gaaccggtac acgccggccc gctccgggcg tccgccgtgg aggaatccca tccggccggg
     7381 aacgtcgaga ctctcgccgt cgggaaggag ctcgaccgcc tgcgacagcg ggacgagccc
     7441 ctccggcgtt cccccgggcg ccgtgacccg cacgaccagc ggcccggtga agaagcgcgc
     7501 accgggaggc agatcgacga agaaccggtc cccctcgcgc cgcagcccac gcccgcgatc
     7561 gacacaatcg acggagacgg cgccggccgc ttcgagcagg cgcgcacggg cggcggggcc
     7621 gagaccgtcg agcgcctcgt acggcaggcc ggccgagagg acccctcccg gcaggcgccg
     7681 ccatcccgcg atcccatccc ccccggcacc aggggcgagg gaggcgaggt ccgaggcgac
     7741 cgccgcgtcg ccgtcgcgca gccgcagatc gaggaagcgc ggcaggacct cggcctgcat
     7801 cgcgcgggca gtctccccgc cgcccgctgc gccgcctgcc gccgtggcgc ctccggaggc
     7861 ggcggccggc tccacacgga tcgtcacggc ggtgcgcccg acatcgccgg aggccgagcg
     7921 cgcgatcacc tccagacggt gcgcgcccgg cggcggcgcg aatgctccgg gcggagccgt
     7981 gccggtcgcc ccgccctctc ccggctcgtc cgcgagggcg ttcccgggga gtcgtccgag
     8041 gcggtaggcg tagcgcgcgg gccccagacg gctgccgcgg tgatcgaaca ggagcccggc
     8101 gatcggtccc tgctcgaacg cgaagcggcg cggctcgaag cggtaggcga ccgcaccatc
     8161 cacccgcacc tcgagaccgg cgagaccggc gcggccgccg ctctccggat cccaggcgga
     8221 caccgacgcg tcgaacgggc cgctcacgac gatcggtcgg tcggcctgga acacgccgtc
     8281 gcgccggcgc aggtcgaacg tcgcgacccg cagccgcccc tcgatcaggc tgtccggcgc
     8341 ggcggcggtc agcgtcagcg actcgaagac cggcgccgga ccgtgggccg ggcgggcgaa
     8401 cccggctgcg aagggatcga tcggacgatc ctgcgcgtcg cgtacctcca cgtgcaggtg
     8461 cggcggcccg acccccgatt cgccggccca ggcgatcacc tgtccccggc tggccttgac
     8521 ccccggatcg agctcgatgt tccccgggaa gcggttcccg gtctcgcgcc gccggcgctc
     8581 gacccgcgat tccaggccga gcgtcttctc ctcgtatcgc tcgaggtggc cgtagacgat
     8641 ccgccggccg tcgcggagcc gcagatacag ggcccggccg tagccgcgcc attccacctt
     8701 gaggcgcacc acttcgccgt cgccggccgc gcgcaccggc agaccggtcg ccccggcggt
     8761 ggacaggtcg aggccggcgt gcaggtggtc gtagcggtac tcgccgaagc tcgagacgat
     8821 ctccttcgcc cgatcgagcg gccagcgcag ggaggggggc gggtcggccg cggccgccgg
     8881 cgccggagtc cccgggagga tcccgcagcc gaggagcgcg agcgccaggg tgcggcgtgc
     8941 cgcgccaggc ccccgccggg acctcacacg aagggagggc gccaggctca tggcggggat
     9001 tctaaccgcc gcgcggccgc acgaccgctg tttgacagcc cggaaacccc cgcactatca
     9061 tccgcccgcc gcggcgcgcg gacgccgcgc gtcccttcga tcacccatgg cctcggaccc
     9121 gttcaagaat ctaaccattc gccgcaaagc cggcgaaacc gtgttcaccg agggggacct
     9181 cgggagcgag atgtacgtcg tgcagtcggg ggaggtgcgc ctcttccggg tgcgcgaagg
     9241 catcaagcag gagctggcgg tcatggagaa gggggatttc ttcggcgagc tgtcggtcct
     9301 cgaggggctg ccgcgcacct cctccgccga ggccttgacc gacgccgatc tgatcgagat
     9361 caacagcacg accttcgacc ggatgatccg ggcgaacatc gagatcgccg tgcgcatgct
     9421 gcgcaagctg agcaaccgac tgcaggaggc gacccacaag ctggaggcga tgagccaggc
     9481 cgacagctcc cgccgtccgc aggcgccggt cgatctcgat cgtcccccgg agcaggaacc
     9541 gccgcccccc gccccgccgc ttccggagcg accggcgcgc cagacggccg gaaagccggt
     9601 ccccgacatc gccgtccccg acggggcgct cggcatgctc atcctcgaag ggggagggaa
     9661 ggtctttccg atccaggcgg cggccgccgt catcggccgc tacgacccgg tgaccgggca
     9721 gcggccggag atcgatctca cccaggtcga catcaaccga tcggtgtcgc gccgccacgc
     9781 ccggctgcag caccaggatg gcgccttcta cctgtcggag gaggtcgggg ccctcaacgg
     9841 cacgctggtg aacgggcggc gcctcgtgcc gggacagccc caggtgctcg ccgccggcga
     9901 tcgcatcgcc gtcggcatgg tggcgctggt ctacaaggcg gcgtcgtgac ccggcctcgc
     9961 gtcctctccg ggttccgacc caccgggccg atgcacatcg ggcacctgaa aggggcgctc
    10021 gagaactggg tgcgcctcca ggacacgcac gactgcttct acgccatctg cgactggcac
    10081 gcgctgacga gcgagtacgc cgacccgagc cgcctgccgg agcacatcga gcagatgggg
    10141 ctcgactggc tggcggccgg gctcgatccc gagcgctcgg tgatcttcgt gcagtcgcac
    10201 gtccccgagc acgccgagct gcacctcctg ctgtcgatga tcgtgccgct gtcgtggctg
    10261 gagcgggtcc cgacgtacaa ggagcagcag gagcagatca cccaccgcga cctgacgaat
    10321 tacggctttc tcggataccc ggtgctgcag accgccgaca tcctgatcta ccgggcgaac
    10381 ttcgtgccgg tcggggagga ccagctggcg cacctggagt tgtcgcggga gatcgcccgc
    10441 cgcttcaacc atctcttcgg cgaggtgttc cccgagccgc aggcgctgac gaccccgacg
    10501 ccgcgcatcc ccggcacgga cggccgcaag atgagcaagt cgtacggcaa cgccatcagc
    10561 ctctccgaca cgccggagga gatccgcagc aagatcctga cgatggtcac cgacccggcg
    10621 cgcaagcgcc gaagcgatcc gggcaacccg gacgtctgcc cggtcttcga cctgcacaag
    10681 gcgttcagca ccgccgcgac gcagcaggag gcggcggccg ggtgcaggag cgccggcatc
    10741 ggctgcatcg actgcaagaa gatgctcctc ggccacctca agccggcgct ctggccggtg
    10801 caggagcgcc gccgcgccct cgatcgcgac cgtggccgca tccatctcct gctggcggac
    10861 ggcgcgcggc gcgcccgcga cgaggcgcgc cgcaccatgg agcgggtgcg ccaggcgatg
    10921 cacctgaccc cgcggaggcc ggcgtgatcc cggaggcgtc ggggagcggc gagtcggccg
    10981 cgggactggc tccgatccgg ctcgacggct tccaggggcc gatcgacgtg ctgctgcacc
    11041 tggcgcggac cggcggcatc gacctgcgcg ggctgccgct cctcgaggtg gcgcggcagt
    11101 gcgacgtctg ggtccggacc ctcgaggcgg ccgatctcga ggcggcgggg gaccatctcg
    11161 tcatggccgc caccctgctg cacctcaagt cccgcatgct cctccctgcg gaaccgctcg
    11221 aatcggcggc cgccgaggcg gacggggagg agatcccggc gcgcctcgcg ggcctcgtgc
    11281 aggagctgcg gcgcgccgcg gagcagctcc aggagcgcga ggcggcgatg gagctcgtct
    11341 acacccggcc gcaggcggtc atggccgagt tcgccggcga ggaggggatc gaggcggatc
    11401 tctacgccct ggtcaacgcc ttccagggga tcctgtcgcg cctcggggcg gtggaccggg
    11461 agtcgaagcg gatctcgcgc gagagcatga gcctgatgga gcgcgtgacg tggctcatcg
    11521 atctgctgca gcgcgagcgc cgggtgcagt tccgcgccct cttcgagggg gtgggggacc
    11581 ggctcacctg catcctgacc ttcctggcgc tcctcgaggt cctgcgcctc cgggtcgccc
    11641 gggcgtggtc gagtcacgac cgcgaagatc tcgtcgtcat cctgaacgag gcgcggcccg
    11701 acgaggccga cgccccggtg cccgacgccc cggatccggc cgcggccggc acccccccgg
    11761 aggaatcgca tgcctgagac gaccgccccg ctccccgacg ccgcgaagct gcgctccatc
    11821 ctggaggcgc tgatcttcgc cgccgaggag ccgctcaccc tcgccgatct cgaggagctc
    11881 ttcccgaacg ccccgaagga gaagcttcag gaagccatcg acgacgtcgc cgcccagtgg
    11941 gacggcgtcg atcgcgggct ccaggtgttg cgggtcgcgg gggggtaccg gctgaccacg
    12001 cgccccgatc tcggcgagtg ggtccgcgcc ctgttccggg cgcgcaaccg gcgacggctc
    12061 tcgggggcgg ccctcgagac gctggccatc gtcgcctacc gccagccgat cacgacgccg
    12121 gagatccagg cgctgcgcgg gatcgatccc gccggcgtgc tgcagaccct gctcgaccgc
    12181 cggctgctca aggtcgtcgg gcggaagaag gtggtgggca agccgatgct ctacgggaca
    12241 acgccggagt tcctgaccca tttcggtctc aacacgctcg ccgatctccc gaccctcgag
    12301 gagttcggcg cgctcgcctc cagcctgggg ctgccgttgg tgcacgaggc gcccacctcc
    12361 accggcgcct tcgaggagga ggacgacgaa ggatcgggcc atgcggacga agaattcgaa
    12421 gatgcgaccg aaagcgacag cgagcgcgac gacgacgggg acgactcctg agccgagcgg
    12481 catccgcctc cagaaggtca tggcggcggc cggcatcgcc tcgcgccgcg aggcggaacg
    12541 gctgatccag gccggccggg tggaggtgaa cggccgcgtg gtgaccgccc tcgggacgcg
    12601 ggtcgacccg gcaggcgacc ggatccgggt ggacgggacc gccatcggcg gggcggagca
    12661 cgcggtcacc gtgctgctcc acaaaccgaa gggggtgctg tccaccgtga gcgatccgac
    12721 cggccgcccc accgtcctcg acctcgtgcg cggcgtgcgc gcccggctct tcccggtcgg
    12781 gcggctcgac tggaacagcg aggggctgct gctcctcacc aacgacggcg acctggcgca
    12841 gcgcctcacg cacccgagca accacgtccc gaagacctac cgcgtcaagg tgaaggggct
    12901 ggtcgggcgc caggcgctcg agcaggtgcg gcacggcctg ttcctcgacg gccggcggac
    12961 ccggccggcg ccggtgaagc ggatctcctc gcagtcgaac acctggctcg aggtcgtcct
    13021 gttcgagggg cgcaagaacc agatccgccg ggtcttcgag cgcctcggcc acccggtgct
    13081 gaagctgcgg cgcaccgcga tcgggtcgct ggtcgatcgt gacttgaagc cgggggagtg
    13141 gcgggagctt tcgcccgccg agatccgggc gctcaaggag agccggtgag cccgcgcatc
    13201 cccgaccacg tgctgcgcat cgcgccgtac gtccccggca agccgatcga ggaggcacag
    13261 cgggagaccg gccgcggcga cgtcgtcaag ctggcgagca acgagaaccc gctcggcccg
    13321 tcgccccacg ccctcgccgc catccgcgcc tcggccgccg aggcccaccg ctacccggac
    13381 ggcagcggct acgcgctgcg cagcgccctg gcggtgcgcc acaacctctc gccgtcgcag
    13441 gtggtcctcg gcaacggctc gaccgagctg gtcgagatcc tcgccaagac cttcctgcac
    13501 gaccggcgca acgccgtcgt cggcgatccg gcgttcatca tgtaccgcat cgccgtcctg
    13561 gcgatgaacg cctccctggt gtcggtgccg ctgcgcgacg accgccacga cctggagtcg
    13621 atggcggagt cctgcacgca gcgcaccgcg ctcgtctaca tcggcaaccc caacaacccg
    13681 accggcacct acgtcgggcg cgccgcgttc gagcgctact tcgcccgcgt cccggcgcac
    13741 gccctgacgg tgatcgacga ggcctactgc gactatgtcg aggcgaagga ctatcccgac
    13801 gggctcgact tcctgcgcgc cgggcgacac gtcgtggtcc tgcggacctt ctcgaagatc
    13861 cacggcctgg cggggctgcg gatcggctac gggctgacct ctccggacgt ggcgcgcgcc
    13921 ctcgaggcgg tccgctcgcc gttcaacacc tcggtcgtcg cccaggcggc ggcgctcgcg
    13981 gccctcaagg accggcggca cgtctcccgc tcccgcgccg gcaacacgcg cgagacccgc
    14041 ttcctcgagg cggcgctcca gaagcgcggc ctcgatttcg tcccgacctc cgccaacttc
    14101 atcctggtac ggaccgggct gggaggggag gagctgcacc agcggctgct ccgcgagggg
    14161 gtcatcgtcc gcccgatgga ggcgtacggt tacaaggatg cggttcgtgt ctccatcggc
    14221 cggcacgaag acaacgagcg tttcctgcgg tcgctcgacc aggtcctcgg ggtgcgccgc
    14281 cggcgtcagc ggtcggtccg ccggcaggag ccttcgtaat cccctgaaaa cagaccgttt
    14341 ttccacttga caggtcctgg gtcgctccgt atgatcg
//
LOCUS       DASRIQ010000544        20805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_458150, whole genome shotgun sequence.
ACCESSION   DASRIQ010000544 DASRIQ010000000
VERSION     DASRIQ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 20805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20805
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458150"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VFD06_13385"
     CDS             complement(<1..131)
                     /locus_tag="VFD06_13385"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZN04570.1"
                     /translation="MSAPLRRPRQGRMVAGVVAGLADRFAMDRTLARVLYVVVSIVSV
                     "
     gene            complement(128..589)
                     /locus_tag="VFD06_13390"
     CDS             complement(128..589)
                     /locus_tag="VFD06_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HZN04571.1"
                     /translation="MPATPSRPVPDRAAPAGYSGTPLPKKLGVKPGSRLVLFGAPAGF
                     ESTLGALPDGARLRRGGTAAADLAVWFVRSRADLLRGIRGRAGTIPPGGLWIAWRKQA
                     SGAASDVGEADVRAAGLSAGLVDYKICAVDPTWSGLKFQTRRSQAKPGRKR"
     gene            complement(558..1433)
                     /locus_tag="VFD06_13395"
     CDS             complement(558..1433)
                     /locus_tag="VFD06_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZN04572.1"
                     /translation="MRWTPGGRSSNVEDRRSQSGGRFPFPGGLRVGGGLGLGTILLLA
                     VLSIVFKTDFLSLLTSGEVPIGGAGSSAPARPVNDPAEEPMVEFVSFVLNDAQAIWAR
                     LLAQAGTPYEDTRLVLFRDAIDSACGFAQSATGPFYCPGDRKVYIDLGFFTELRERFG
                     APGDFAQAYVLAHEIGHHVQTLLGTEAAVRRAQREGGEGSALSVATELQADCYAGIWA
                     HWTANEAHLEEGDIEEGLGAAAAVGDDRLQRQGGGRVNPDAFTHGSSRERAAWFRRGY
                     ESGSLDACDTFASRP"
     gene            complement(1481..2329)
                     /locus_tag="VFD06_13400"
     CDS             complement(1481..2329)
                     /locus_tag="VFD06_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN04573.1"
                     /translation="MTLLFAALALVAFAANSILCRLALAEGRIDAASFTTLRLLAGVL
                     VLLPLALSRRPAAMGRARLGGTFAAGLMLALYALPFSFAYLSLTAGTGALLLFGAVQG
                     TMIAAGLFTGERPGLVEWTGSAIALAGLVILVAPGVEAPPPVGALLMVLAGAAWGIYS
                     LLGRAAADPIAATAGNFLRAAPWALAASLAALGAAHADRRGAFLAILSGAVTSGLGYV
                     AWYAALPGLGAMRAATAQLAVPAIAALGGVLLLGEQMTPRVGGAAALVLGGVALALRG
                     RSASSR"
     gene            complement(2326..3192)
                     /locus_tag="VFD06_13405"
     CDS             complement(2326..3192)
                     /locus_tag="VFD06_13405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZN04574.1"
                     /translation="MDQRIIDLYDEYTHRPLERRVFLERLAMLAGSAAAALALLPILQ
                     NDYAHAAQVSPDDPGIDARRVEYAGASGKIKGYLARPRKGEKRRPAVIVIHENRGLNA
                     HIEDVTRRAALAGFLALAPDCLSPVGGTPADEDQAREKIGALDKEAVVKDLVSTRRWL
                     AEHKSSSGRVGAMGFCWGGGMVNLLAAADPDLAAGVVFYGMSPPLEKVPAIRAKMLLN
                     YAGLDERINAGVPAYEEALKAAGVTYTLHMYDGAQHAFHNDANAARYDAAAAALAWKR
                     TVEFLTGALASA"
     gene            complement(3242..3955)
                     /locus_tag="VFD06_13410"
     CDS             complement(3242..3955)
                     /locus_tag="VFD06_13410"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN04575.1"
                     /translation="MQDFGGRARFVSENYGDSDLASRFGITHYPAIFVDDVLVATPRD
                     FGFFGKGEKEGDGRYTPLRTAASHDRFRADLARMIDLILSGRKDLAQGAAAQPSAGLA
                     ALPDVALSGLDGTTRRRTDLEGKVVLVEFWATWCPPCRSTLAWLADLKKRHGDRLEVL
                     ALAVDSEEEAVRKIAASVSPDLTWAMTTPEIGRGFGDVTSVPTLFLFGPDGRAAATFY
                     GAPPTLHADAEGRIDALLR"
     gene            complement(3995..5152)
                     /locus_tag="VFD06_13415"
     CDS             complement(3995..5152)
                     /locus_tag="VFD06_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04576.1"
                     /translation="MRRTDATPPIAATAVLAATAVLAAFPSLPRAAAAGPEPATARPS
                     PAADPLATEIERLSGLLKTAPDDDFWKQIKNGGGVVLGRAEQALKDGRRLLALQRLFV
                     ARMEVASALWTQARPEKERTDLAAFEAAWTRLGEARAKEFRMPAPAAFDGVRPALVRG
                     LAEAAAAQAGTLYRASLDYGRSTEPLYGLFYAGTGEAQIGFAAFCRSLADPAAPKPPA
                     LRSFAPELEALQEQLLKSYKPPVSIDRHGEFITANAALKEARELDDAGLRYGALVKLL
                     DATMRSGVLSQPPPGDAASPAPSAEELQRMLAPFKERLAARGKDHTIGRTYLEAAEAN
                     TPGATGANPAVALAIARDVLPRYFAALEPALKAPPRPTPTVSVTLVRWPYT"
     gene            complement(5254..6738)
                     /locus_tag="VFD06_13420"
     CDS             complement(5254..6738)
                     /locus_tag="VFD06_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN04577.1"
                     /translation="MTRTAVRATILALSLPVFAAAAQAPAPGSDPARRFTVTPLATGV
                     YAVIRDDRPGFMVDANNLFIVNDADVVVVDSNGAPSITRQVVAALKRLTDKPVRYVVN
                     THYHDDHIRGNQVWREAYPGVEFVAHEFAREYLPGQGAINRKNFLTGAPGFLQDLKTS
                     LQAGKNVAGAPLTPEERESLESDIALASLVLSEGAEAETVLPTITVRDRLTLVRGTRV
                     IDILHLGPGHTSADLVVHLPKERIVATGDLVAWPVPLVGNPQSHPATWSTALDRLREL
                     HPATLVPGHGPVLRDDAYVKQIESMFAVIAAQADAAARRGETGEAARAAVDVAPFRAT
                     LAGDSPVRRALFDMYVAQPAVGAALRDASASGAAALAADVRAAETAFARTMAARDHAA
                     FTAMLADEAVFFGSQRILRGRAAVAEGWRPFFDGTQAPFSWAPERVEVLDSGTLALSS
                     GPVFDPGGTRTGTFNSVWRRGDDGRWRIVFDSGCPPCAPPAPAK"
     gene            complement(6898..8865)
                     /locus_tag="VFD06_13425"
     CDS             complement(6898..8865)
                     /locus_tag="VFD06_13425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN04578.1"
                     /translation="MKGHPLPPRIAAALLLVAGAILRIAEYAEGRSLWLDETLLSLNV
                     IERSWAGLLAPLDYQQGAPIGFLLLQKAAVLVAGSGEYGLRLIPFLAGIATLVFGWLL
                     ARRWLGPEGGLVALGLLALCDPLIYFSAEAKQYGVDACVAVVLLWAGARALQDRGRRD
                     FVLLACLGSALLWFSHPAAFVLDGIAAMLAVAAIRARDRGALLRLAMVGASWTASFLL
                     CCALSLRRLAANPFFLDFWAPWFVPSIGDLPWFARRLASHFAEFLDLSPSPPLFFAWL
                     GIGAVACATLRPAFAVAGLASFLSAACASALSRYPLSERFLMFFVPIALLVLAEGLQL
                     VRSMAGRRVPGAGAVVLIAAFGFPAWRAVQEIRAPRVVQDFKPILADLKDRVRPDDWI
                     YVSHLASYPWRYYAGRYGFDGDVEVPRDYADPRLVLEWADRRRLFEAPGRNPVLLGSV
                     SHFTSDRVRDVILDLQALLGRPRAWVVISHGAGGRNAGRSILAYLDRVGRRLEQVRRP
                     GLWGGCEAVLYDLSGAVSAAAAPDEGPADVAALLRGAGAALRAGDAERSLRLFDRLEA
                     SATLGDQALFAKGQAQQLYGDDDGAAATYRALLADNPGSVRARYFLGEALVSLDRCAE
                     ATGELRRALAERPDLEGAWDALARCRMRAPR"
     gene            complement(8862..10328)
                     /locus_tag="VFD06_13430"
     CDS             complement(8862..10328)
                     /locus_tag="VFD06_13430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN04579.1"
                     /translation="MLFGDVLRLHGYTRHDLWLDEANSVAIARHAFSEIPALLARDSS
                     PPIYYWLLHLWIQVMGESEAAVRLPSVILGAIVIAATAVLAHRLAGERAAVAAAWLIA
                     VTPMAVQFSQQARMYMLLPLFAIVATERFLVYQRDGAIGALVAHALALVACFYTHNWG
                     LLLFPGFVVAAALAPRARWRAWGVAAALALVSYVPWIPVLRQQIAGSSYRFIQVLGES
                     RVYLLPLETLWLFASGVGNPGEKPGSLMPDFAGFLGAGIWVAVAATALVLRPVLKPAA
                     FQVLAPALAPLCLAAAWQAIGSRPIYFLGRYEIMVLPLVIAVVVSGAVLLFPWRWVTA
                     LMAAWAVVLAALSWNYTSAVRRTYPEPIMARTLAPVLRPGDRVVFTGLFRATTEYYLR
                     RAAAPPYEGASFPPDVADHLGWHFDDLYDVADPVLAEAARRNCPTPGHRTWVVGSNDP
                     TTFLLLRVLQTCSIMTKPFAAKGRPASSLFLAEPSGPS"
     gene            10544..10942
                     /locus_tag="VFD06_13435"
     CDS             10544..10942
                     /locus_tag="VFD06_13435"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 family protein"
                     /protein_id="HZN04580.1"
                     /translation="MKTRIVSVVVVFVVAMLLGFVVHGVLLANDYAQLTSVMRPPDEA
                     QQTFPWMILAHLLIAVGYTWIYVRGKEPKPWLGQGVRFGLAVAVLSVIPMYLIYHAVA
                     QFPLSLALKQIVFDTIGCIVLGIVVARVNR"
     gene            complement(10975..11796)
                     /locus_tag="VFD06_13440"
     CDS             complement(10975..11796)
                     /locus_tag="VFD06_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN04581.1"
                     /translation="MSSSDAKFAGSIPDLYEAHLGPLLFEPYADDLAARLPIPTGAGA
                     FRVLEIAAGTGILTRRLRRRLPPTATLIATDLNEGMLQVARRRLEGIDGIEFRQADAM
                     ALPFPDASFDAVACQFGLMFMPDKPKAVREVRRVLTPGGTYLLNTWGSLHDNPASALT
                     HEVLASLFPSNPPPFLKTPFGDHDPQALTTLLSEAGFTEIAITPVRIEGQAASAESAA
                     TGLIAGSPVFNQIVERGSPTPAEIIPILTVEFARAFGDRPMRSPMLALAITARRP"
     gene            complement(11832..12239)
                     /locus_tag="VFD06_13445"
     CDS             complement(11832..12239)
                     /locus_tag="VFD06_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04582.1"
                     /translation="MRAVVLMLLVLMLAVPSIAAPPRLGIEIAAPEHKVLDGPNADGD
                     LELSVKVMVKNTLDMDESVQIVVQALDRDEYEVLEVVLAGPVRAGKARPLTQSALLQR
                     DVYRTIVAWRIEEVTITALQADGAPESKASVRR"
     gene            complement(12278..13201)
                     /locus_tag="VFD06_13450"
     CDS             complement(12278..13201)
                     /locus_tag="VFD06_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HZN04583.1"
                     /translation="MTIRPLPVLAVVIMSVAAPSFAQEPSGGSPVSAEEFEAGLGYQT
                     GTITLEDGLATLHLPETFRFIPPEGSRRLLTEAWGNPPQAAQGVLGMLVPRDTSPLSE
                     DGWGVVISFDEDGYVDDKDAASIDYDDLLEDMQEGMKAENERRKKAGFGSMTLVGWAE
                     PPHYDAATHKLYWAKDLNFEGSDQHTLNYNIRILGRRGVLVLNAVASMGQLATVREQA
                     AALPAAVEFNEGHRYADYLPGKDKAAAYGVAGLILGATAVKAGLFKALWLGILAFKKF
                     LLVGLLALIPVLKRLFVRKPAGAAAAGASEP"
     gene            complement(13218..13466)
                     /locus_tag="VFD06_13455"
     CDS             complement(13218..13466)
                     /locus_tag="VFD06_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04584.1"
                     /translation="MSEVLRRVRAAALPSLLLLVAPPPAAGEDPGPFADLSGEWNGTI
                     QVVTAPACTADFGGRTLFPACSKGHCVIHTYFKLARPH"
     gene            13536..15197
                     /locus_tag="VFD06_13460"
     CDS             13536..15197
                     /locus_tag="VFD06_13460"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN04585.1"
                     /translation="MPRKRIVALTLAALSFGAAPAFAQSLNEPALIVQSVVSGLEQPT
                     SMAFLGPDDILVLQKADGRVRRVTGGVLQAAPVLDVAVDSLSERGLLGIAVDPDFAVT
                     RHVYLYYTESSTPLDTTGEANPLGNRIYQYDWDGSALVNPALILDLPWSTGPNHDGGV
                     IAFGPDGFLYAVIGDLNRSGKLQNIPLGPDPDDTGVILRIDTNGLGAPDNPFYTPAEP
                     AARMSRYFAYGVRNSFGLAFDARTGDLWDTENGPNSYDEVNRVIPGFNSGWNRIMGPD
                     SRDPQGIGDLWVAPGSIYRDPEFSWFIPVAPTALVFPESPIVGCALQHELLVGDSNCG
                     QIYRFKLNAARDALALSSAPLVDRVMDNATNRCLDEVGEIAFGQSFGSVTDLENGPDG
                     MIYVVSITTGRIFRIAPRPGFFPDADGDGADDACDCAAGDAGAYAAPGEIPRQRLLGA
                     APHTVFWDSRTGAFGPGTTTTIVSGLAGGLRASGGYAGACDLAVGVAGTSFVDPRAAP
                     PVGEAYYYLVRAQNSCGQGTFGDGTPVPDPRDELDTGTRPSCGSP"
     gene            complement(15194..16318)
                     /locus_tag="VFD06_13465"
     CDS             complement(15194..16318)
                     /locus_tag="VFD06_13465"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN04586.1"
                     /translation="MSRIGGNLASGRAALAAAVAAFGLAIASAVPDRRTENLVLLTLD
                     GVRVQEVFGGMDPVVAFGRSKRSGIYDPERARRLYWRDDAEARRKALWPYFWGTLAPQ
                     GVVLGDAGRGSRATLRNPHGFSAPGYLELLTGRFHPDVTTNDARRYPYPTVLQAIRRG
                     LGLRPEQVAVFSTWDALGLFASSEPDDLFANAGYARVPPALSNPAMDALGDYQEHVMA
                     LWEGYRTDMPTFHLALEYLKARRPRVLYLALGETDDWAHARRYDRLLDCLRLTDDYMR
                     ILWETLQSLDGYRDRTTLVVTTDHGRGRTPKDWVDHGEGIAGSEEIWIAIIGPDTPDR
                     GVVGPAPTVHQADVAATMLELLGLPLASLPPEAGPPIRLP"
     gene            16550..19996
                     /locus_tag="VFD06_13470"
     CDS             16550..19996
                     /locus_tag="VFD06_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN04587.1"
                     /translation="MRACVVVVALVISALLCTAVHGQETTSGTIAGKVIDPAGKPIAG
                     AIIIAQSDSGPRTATTDDAGNYVLPFLRPGTYTVRVEAPSGFNTALQEGVIVGLNQRV
                     PLDFTLTPGKVEEITVTSKPLIDSTSTASGTNIRYDEFVNSVPIGRSFTDTYGVASGV
                     VSGRGTGQGNYSISGASGLENSYLIDGVNVTNTGYGGIGAYNIVYGSLGTGVTSEFLD
                     EVQIKTGGFEAEYGQALGGIINTIVKSGTNDFKGSVAWYATPQGATSDYTVTELQAGA
                     ASTVEQGVNDFAFQVGGPIVKDKVFYFIAYNPVITTTGREAQTLANPMFAPAEAGVSV
                     FDETAPTGFGVPAALAFPSAGRTLERRRQANNYAAKISFQMTENQNLELTAFGDPAAG
                     DMGPQRDIAPLFGDFADGGGESSVRYGSNNLALKWNAIVTPRFFLEGILAHHDGFFRE
                     TTGLNQYRYIDYRNNLEFRRGATTYTDPVLGPTPFTASATLPTEGGVGFITSQDDQQT
                     QAILKLTNTFEQHEVKYGVEYDTIRYRENAAYTGPSFNIQLPVSFYDPATLTVSPVDA
                     DSDGTQDFLPIASRGGAIVQVRNGAGADPTVAFDSANNFRVTRARMGPELPYTNANEW
                     NGFVQDTWSITSRVSLRAGVRYTQETIDGAGTFTLPFATSTVEQEVTPGNTVESRIFT
                     PGTSTYTPNSYTFAGNWAPRLGVSWDVLGSGRSRLYVNVARYFQRVPSDLAVRAFSNE
                     VGISLQEFNTRDLSVPRTAGTDGCVDNGGTATTTCATSFPVFTQGVDQTNVVGGVKLP
                     YEDEISGGYAFELNDSSSLEVRAIFREQGRVLEDTQVNAIEQIQNFYYGTAYGYPYDP
                     FGGSPATPASTTFPARTFGTYSLANPGTPQIPQGLAPGINFPKPERTYKALEAIYTKR
                     FDPHWSLFANYRLARLEGNYEGLFRNDNGQSDPNITSLYDFPSSPLMQGQFIKGPLPA
                     DATHVVHIFPSYVFDMKLRVGANVSWSSGFPRTSMLAHPIYQNAGEIPGIDPVYAYWQ
                     DDGTGTLALATTSDLSSALADPNAMSNIFLRSYTPVKRGNLGRLPDLWTLDLHADYPI
                     AVGKTSLAVMFDVFNVTDEDTVTNFNDNVELTAGVTDPDFTAPLTYQAPRTVRLAARW
                     SF"
     gene            20150..>20805
                     /locus_tag="VFD06_13475"
     CDS             20150..>20805
                     /locus_tag="VFD06_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HZN04588.1"
                     /translation="MPDEKSIADAFRRWGYLAADLDSLGRLPPFTHPEILDVAQGKTA
                     AAAERYRKIYCGPIGAEFMHLRRGEVSDWVAARMEREPRAPDRPAILRRLAQAELLEQ
                     FMHRRWVGSKRYSLEGSASVLVALDNILDEAASAEVALIAMSHRGRLNVVVNIVGRAP
                     GDVFARFEEGDPRAFLGGGDVRYHLGATGEYRTVSDRIIRLHLVSNPSHLEAVDPVMM
                     G"
BASE COUNT         3230 a         7337 c         7409 g         2829 t
ORIGIN      
        1 acggagacga tcgagaccac gacgtacagc acccgcgcca gagtgcggtc catggcgaag
       61 cggtcggcga gaccggccac cacccctgcg accatccgtc cctgacgcgg ccggcgcaac
      121 ggagcgctca tcgtttcctc cccggtttcg cctgacttcg gcgcgtttga aacttgagtc
      181 ccgaccacgt cggatcgacg gcgcagatct tgtagtcgac gagcccggcc gacagtcccg
      241 cggcccgcac gtccgcctcc ccgacgtccg acgcggcgcc ggacgcctgc ttcctccagg
      301 cgatccagag gcctccggga gggatcgttc cggcccgtcc gcggatgccg cgcaggagat
      361 cggcgcgcga gcggacgaac cagaccgcga ggtcggcggc cgccgttccg ccgcgccgca
      421 gccgggcacc gtccggcagc gcgccgagcg tcgattcgaa gccggccggc gcgccgaaca
      481 gcacgagacg cgatcccggc ttgaccccga gcttcttcgg aagcggggtt ccggagtatc
      541 cggccggcgc ggcgcggtca gggacgggac gcgaaggtgt cgcaggcatc gaggctcccc
      601 gattcgtagc cgcggcggaa ccaggccgcc cgctcgcggg acgatccgtg cgtgaaggcg
      661 tccgggttga cgcggccccc gccctggcgc tgcaggcggt cgtcccccac ggcggcggcc
      721 gccccgagtc cctcctcgat gtccccctcc tcgaggtgcg cctcgttcgc ggtccagtgg
      781 gcccagatgc cggcgtagca gtccgcctgc agctcggtcg ccaccgacag ggctgagcct
      841 tcgcccccct cgcgctgcgc ccggcgcacc gccgcctcgg tgccgaggag cgtctgtacg
      901 tggtggccga tctcgtgcgc caggacgtag gcctgcgcga aatcgcccgg ggcgccgaag
      961 cgctcccgca actccgtgaa gaacccgagg tcgatataca ccttgcggtc gccgggacag
     1021 tagaacggcc cggtggccga ctgcgcgaag ccgcaggcgg agtcgatggc gtcgcggaac
     1081 agcacgaggc gcgtgtcctc gtacggcgtc ccggcctgcg ccaggaggcg cgcccagatc
     1141 gcctgcgcgt cgttgagcac gaaggagacg aactcgacca tcggctcctc ggccggatcg
     1201 ttcaccggcc gggccggcgc ggacgaaccg gcccctccga tcggaacctc gccgctcgtc
     1261 aggagcgaga ggaaatcggt cttgaagacg atgctcaaaa cggcgagaag gaggatcgtg
     1321 ccgaggccga ggccgcctcc gacccggagc ccgccgggaa aagggaaccg gccgcccgat
     1381 tggctgcgtc ggtcctcgac attgctgctg cgcccgccgg gtgtccagcg catcgtccgg
     1441 cgatcttaca tgacggcggc gcaccctccc gccgtggaat ctaccgagac gatgcggacc
     1501 ggccccggag cgccagcgcg acgccgccca gaacgagggc ggcggcgccg ccgacgcgtg
     1561 gcgtcatctg ctccccgagg agcagcacgc cgccgagggc ggcgatcgcc ggcaccgcca
     1621 gctgcgcggt ggcggcgcgc atcgcgccga gccccggcag ggcggcgtac caggcgacgt
     1681 agccgagccc cgaggtgacc gcccccgaca ggatcgccag gaatgcgccc cggcgatcgg
     1741 cgtgcgcggc gccgagcgcc gcgagactcg ccgcgagtgc ccagggcgcg gcgcggagga
     1801 agttgccggc ggtcgcggcg atcggatcgg cggcggcgcg gccgagaagc gaatagatcc
     1861 cccaggccgc ccccgcaagc accatcagca gcgctccgac cggcggagga gcctcgaccc
     1921 cgggggcgac caggatcacc agcccggcga gagcgatggc ggacccggtc cactcgacaa
     1981 gacccgggcg ctcccccgtg aaaagcccgg ccgcgatcat cgtcccctgc accgccccga
     2041 acagcaggag cgccccggtg ccggcggtga ggctcagata cgcgaaggag aagggcaggg
     2101 cgtagagcgc cagcatcagc cccgccgcga aggtgccgcc gaggcgcgca cgtcccatcg
     2161 ccgccgggcg gcgcgacagc gccagcggca ggaggacgag cacgcccgcg agcagccgca
     2221 gcgtggtgaa ggaggcggca tcgatgcgcc cctccgccag ggcgagccgg cagaggatcg
     2281 agttggcggc gaaggcgacg agcgccaggg ccgcgaacag gagtgtcaag cggaggcgag
     2341 ggccccggtc aggaactcca cggtccgctt ccaggcgagc gccgccgcgg cggcgtcgta
     2401 gcgcgctgcg ttcgcgtcgt tgtggaaggc gtgctgcgcg ccgtcgtaca tgtgaagcgt
     2461 gtacgtgacg cccgcggcct tcagcgcctc ctcgtacgcc ggcacgccgg cgttgatccg
     2521 ctcgtccagc ccggcgtagt tcaggagcat cttcgcgcgg atggcgggga ccttctccag
     2581 cggcggcgac atcccgtaga agacgacgcc ggccgcgagg tccgggtccg ccgccgcgag
     2641 caggttcacc atcccgcctc cccagcagaa acccatggcc ccgaccctgc cgctcgagct
     2701 cttgtgctcc gcgagccagc ggcgcgtcga gaccagatcc ttgacgaccg cctccttgtc
     2761 gagcgcgccg atcttctcgc gcgcctggtc ctcgtcggcc ggggttccgc cgacagggga
     2821 gaggcagtcg ggagcgagcg ccaggaagcc ggccagcgcg gcccggcgcg tcacgtcctc
     2881 gatgtgggcg ttgaggccgc ggttctcgtg gatcacgatg accgcagggc gacgcttctc
     2941 ccccttgcgc ggccgcgcca ggtatccctt gatcttcccg gaagctcccg catattcgac
     3001 gcggcgggcg tcgatcccgg ggtcgtcggg ggacacctgc gccgcgtggg cgtagtcgtt
     3061 ctggaggatc ggcaggaggg cgagggccgc cgcggcggag ccggccagca tcgccagacg
     3121 ctcgagaaaa acccggcgct cgagcggccg gtgcgtgtac tcgtcgtaga ggtcgatgat
     3181 gcgctggtcc atgggctcct cgtggaaggg ccccggcggc cccgggacat tctgcctcag
     3241 gtcagcgcag aagcgcgtcg atgcgcccct cggcgtcggc gtgcagggtg ggcggggccc
     3301 cgtagaaggt cgcggcggcg cggccgtccg gaccgaagag gaacagggtc gggaccgagg
     3361 tgacgtcgcc gaagccgcga ccgatctccg gcgtggtcat cgcccaggtg agatcggggg
     3421 agacggatgc cgcgatcttc cggaccgcct cctcctcaga gtccaccgcc agcgccagca
     3481 cctcgagacg gtcgccgtgg cgcttcttga ggtcggcgag ccaggcgagg gtgctgcggc
     3541 acggcgggca ccaggtcgcc cagaactcca cgagcaccac ctttccctcg aggtcggtgc
     3601 ggcggcgcgt ggtgccgtcg agcccgctga gcgccacgtc gggaagcgcg gccagaccgg
     3661 cagagggttg cgcggcggcg ccttgggcga gatccttccg gccgctcagg atgaggtcga
     3721 tcatccgcgc gaggtcggcg cggaagcgat cgtggctcgc ggcggtccgg agcggcgtgt
     3781 agcggccgtc cccttccttc tcccccttgc cgaagaaccc gaagtcgcgc ggggtcgcga
     3841 ccagcacgtc gtccacgaag atcgccgggt agtgggtgat gccgaagcgg cttgcgagat
     3901 ccgagtcgcc gtagttctcg gagacgaacc gcgcccgccc tccaaagtcc tgcacgacac
     3961 tctgcgccag cagacctgcc gggtcggaga tgtttcaggt gtacggccag cggacgagcg
     4021 tcacgctgac cgtcggggtg gggcggggcg gcgccttaag tgccggctcg agggccgcga
     4081 agtagcgcgg cagcacgtcg cgcgcgatgg cgagggccac cgcgggattg gcgccggtcg
     4141 cgccgggggt gttggcttcc gccgcttcga gataggtgcg gccgatcgtg tggtccttgc
     4201 cgcgcgcggc cagccgttcc ttgaacggcg cgagcatccg ctggagctcc tcggcggaag
     4261 gcgccggtga ggcggcgtcg cccggcggcg gctggctcag gacccctgac cgcatcgtcg
     4321 catcgagcag cttcaccagg gcgccgtagc gcaggcccgc gtcatccagc tcgcgcgcct
     4381 ccttgagcgc cgcgttggcg gtgatgaact cgccgtggcg gtcgatcgag accggcggct
     4441 tgtagctctt caggagttgc tcctgaagcg cctccagctc gggagcgaac gaacgcagcg
     4501 ccgggggctt cggagcggcc ggatcggcga ggctgcggca gaaggcggcg aacccgatct
     4561 gcgcctcgcc ggtaccggcg tagaacaggc cgtagagcgg ctcggtgctg cgcccgtagt
     4621 cgaggctggc gcgatagagg gtcccggcct gtgccgctgc cgcttcggcg agcccgcgta
     4681 cgagggcggg tcgcactccg tcgaaggcgg ccggcgcggg catccggaac tccttcgcgc
     4741 gcgcctcgcc gagacgcgtc caggccgctt cgaaagcggc gaggtcggtc cgctccttct
     4801 cgggccgcgc ctgcgtccag agcgccgaag ccacctccat tcgggcgacg aacagccgct
     4861 gcagcgccag gagccgccgg ccgtccttga gcgcctgttc ggcacgcccg agaacgacac
     4921 cgccgccgtt cttgatctgc ttccagaagt cgtcgtccgg agccgtcttc aggagtcccg
     4981 agaggcgctc gatctcggtg gcgagcggat cggcggccgg ggacgggcgt gccgtcgccg
     5041 gctcgggacc ggccgcggcg gcgcgcggaa gcgaaggaaa cgccgcaagc accgcggtgg
     5101 cggcgagcac cgcggtggcg gcgatgggcg gggtcgcgtc ggtcctgcgc atcgttccgg
     5161 acctccgagg aacggccggc gaaggacaac ccccgcccga agcctcaacg cgccggcatc
     5221 tcccgtggtt catcgcgttc ctcatggcgg acctcacttg gccggcgccg gcggggcgca
     5281 ggggggacag ccgctgtcga agacgatgcg ccagcggccg tcgtccccgc ggcgccagac
     5341 ggagttgaag gtgccggtgc gcgtgccgcc ggggtcgaac accggtccgc tgctcagggc
     5401 gagggttccc gagtcgagca cctcgacccg ctccggggcc cacgagaagg gggcctgggt
     5461 tccgtcgaag aacgggcgcc atccctccgc gacggccgcc cggccgcgca ggatccgctg
     5521 cgagccgaag aacaccgcct cgtccgcgag catcgcggtg aaggcggcgt gatcgcgcgc
     5581 cgccatcgtc cgggcgaagg cggtctcggc ggcccgcacg tccgccgcga gcgcggcggc
     5641 gccggaggcg gatgcgtcgc gcagcgccgc cccgaccgcc ggctgcgcca cgtacatgtc
     5701 gaagagggcc cgcctcaccg gggagtcgcc cgcgagcgtc gcgcggaacg gcgccacgtc
     5761 cacggccgcg cgcgccgcct ctccggtctc gccgcgccgc gccgccgcgt ccgcctgggc
     5821 cgcgatcaca gcgaacatcg actcgatctg cttgacgtag gcgtcgtcgc gcagcaccgg
     5881 cccgtgtccc gggaccaggg tggccgggtg gagctcgcga agccgatcga gtgcggtgct
     5941 ccacgttgcc gggtgcgact gcgggttccc cacgagcggg acgggccagg cgacgaggtc
     6001 tccggtggcg acgatgcgtt ccttcggcaa gtgcaccacc aggtcggcgc tggtgtggcc
     6061 ggggccgagg tgcaggatgt cgatgacccg cgtgccgcgc acgagcgtca ggcgatcccg
     6121 caccgtgatg gtcgggagga ccgtctccgc ctcggcccct tcgctcagca cgagtgaagc
     6181 gagggcgatg tcgctctcga gactttccct ctcctccggc gtcagcggcg cgccggcgac
     6241 gttcttcccc gcctggagcg aggtcttcag atcctgcagg aagcccggcg ccccggtgag
     6301 gaagttcttc cggttgatcg ccccctgtcc cggcaggtac tcgcgcgcga actcgtgcgc
     6361 gacgaactcc acgccggggt aggcctcgcg ccacacctgg ttcccgcgaa tgtgatcgtc
     6421 gtggtagtgg gtgttgacga cgtagcgcac cggcttgtcg gtcagccgtt tgagggcggc
     6481 caccacctgc cgggtgatcg acggcgcccc gttgctgtcc accaccacga cgtcggcgtc
     6541 gttgacgatg aacaggttgt tcgcatcgac catgaagccg ggccggtcgt cgcggatgac
     6601 ggcgtacacc cctgtggcca gcggcgtcac agtgaaccgg cgggccgggt cggaacccgg
     6661 ggccggtgcc tgggcagcgg cggcgaagac gggcagggac agtgcgagga tagtggcgcg
     6721 gacggcggtt cgcgtcacgg acggtggccc tcctcccgag gacggggcgg acatcaattg
     6781 cgtacccgaa cgcgtccgcc ggggccacca ccggagccgc gcgggccgca tcataggagc
     6841 cggtccgggt ccggcgcaag tgcggactcg gacaggggtc cgacggatcc ggacaactca
     6901 gcgtggcgcg cgcatccggc agcgggcgag cgcgtcccac gccccctcga gatcggggcg
     6961 ctccgcgagg gcccggcgca gctcccccgt cgcctcggcg cagcggtcga gcgagacgag
     7021 cgcttcgccc aggaagtaac gggcacggac gcttccggga ttgtcggcga gaagggctcg
     7081 gtaggtcgcg gcggccccgt cgtcatcgcc gtacagctgc tgcgcctgcc ccttcgcgaa
     7141 cagcgcctga tcaccgaggg tcgcgctcgc ctcgaggcgg tcgaacaggc gcaggctccg
     7201 ctcggcgtcc ccggcgcgca gcgccgcccc cgccccgcgc aacagcgccg ccacgtcggc
     7261 gggcccctca tcaggcgccg cggcggcgga gaccgctccg ctcaggtcgt acagcaccgc
     7321 ttcgcagccc ccccagaggc ccggtctgcg gacctgctcg agccggcggc cgacccggtc
     7381 gaggtacgcg agaatcgagc gccccgcgtt cctgccgccg gcgccgtggg agatcaccac
     7441 ccaggcgcgc ggccggccga ggagcgcttg gagatcaagg atgacgtcgc gcacccggtc
     7501 ggacgtgaag tgggaaaccg acccgaggag gaccggattg cgtcccgggg cctcgaagag
     7561 ccggcgccgg tccgcccatt cgaggaccag ccgcgggtcg gcgtaatcgc ggggcacctc
     7621 cacgtcgccg tcgaacccgt agcgcccggc gtagtagcgc cagggatacg aggcgaggtg
     7681 cgacacgtag atccagtcgt ccggccggac gcgatctttc aggtccgcga ggatcggctt
     7741 gaagtcctgg accacgcgcg gcgcgcggat ctcctggacg gcgcgccagg cagggaagcc
     7801 gaatgccgcg atgaggacga ccgccccggc ccccggcacg cggcgtccgg ccatgctccg
     7861 gacgagctgc agcccctccg ccagcaccag gagggcgatc ggaacgaaga acatcaggaa
     7921 ccgctcggaa agggggtagc gcgacagggc cgacgcgcac gcggcggaga ggaagcttgc
     7981 gagaccggcg acggcgaagg cgggccggag cgtggcacag gcgaccgccc cgatcccgag
     8041 ccaggcgaag aagagcgggg gagacgggct gagatcgagg aactccgcga agtgactcgc
     8101 cagccgccgc gcgaaccagg ggaggtcgcc gatcgacggg acgaaccacg gcgcccagaa
     8161 gtcgaggaag aagggattcg ccgcgagccg gcgcaggctc agcgcacaac agagcaggaa
     8221 ggaggcggtc caggaggccc ccaccatcgc gagacggaga agcgcgcccc ggtctcgcgc
     8281 ccggatcgcc gcgacggcga gcatcgctgc gatgccgtcg aggacgaagg cggccgggtg
     8341 ggagaaccag agcagggccg acccgagaca cgcgagcagg acgaagtccc ggcggcccct
     8401 gtcctgcagc gcacgcgctc ccgcccagag gagcaccacc gccacgcagg catcgacacc
     8461 gtactgcttc gcttcggcgg aaaagtaaat cagcggatcg cacagggcga ggaggccgag
     8521 cgcgacgagt ccgccttccg gtccgagcca gcgccgcgcc aggagccagc cgaagaccag
     8581 ggtcgcgatt cccgccagga aggggatgag ccgcaggccg tattcccctg accccgcgac
     8641 gagcacggcg gccttctgca gcaggaggaa accgatcggg gccccctgct ggtagtcgag
     8701 aggcgcgagg agcccggccc acgatcgctc gatgacgttc agcgacagga gcgtctcgtc
     8761 gagccaaagg gagcgcccct cggcgtactc ggcgatgcgc aggatcgccc ccgccaccag
     8821 aaggagggcg gcggcgatcc gtggtgggag ggggtgtccc ttcaagacgg cccggaaggc
     8881 tccgccagga acaggctgct cgcgggccgc cccttcgcgg cgaacggctt cgtcatgatg
     8941 gagcaggtct ggagcacccg gagcaggagg aaggtcgtgg ggtcgttcga tcccaccacc
     9001 caggtccggt ggcccggcgt gggacagttc cgccgcgcgg cctcggcgag caccgggtcg
     9061 gcgacgtcgt agaggtcgtc gaagtgccag ccgagatgat ccgcgacgtc gggcgggaag
     9121 gaggcccctt cgtagggagg ggcggcggcg cggcgcaggt agtactccgt cgtggcccgg
     9181 aaaaggcccg tgaacacgac gcgatcaccg ggacgcagca ccggggcgag ggtgcgcgcc
     9241 atgatcggct cggggtaggt gcggcgcacg gcgctcgtgt agttccacga cagggccgcg
     9301 aggacgaccg cccacgccgc catcagcgcc gtgacccagc gccacgggaa cagcagcacc
     9361 gccccggaca ccaccaccgc gatcacgagc gggagcacca tgatctcgta gcgtccgagg
     9421 aagtagatcg gccggctgcc gatcgcctgc cacgcggcgg caagacacag cggcgcgagg
     9481 gccggggcga gcacctggaa cgccgccggc ttcaggaccg gcctcaggac gagcgccgtg
     9541 gcggcgacgg cgacccagat cccggcgccg aggaaccccg cgaagtcggg catgaggctg
     9601 cccggcttct ctcccgggtt gccgacgccg ctggcgaaga gccacagcgt ctcgagcggc
     9661 agcaggtaca cgcgggattc cccgaggacc tgaatgaacc ggtaggagct ccctgcgatc
     9721 tgctggcgca gcaccggaat ccacggcacg taggagacga gcgcgagcgc cgccgccacg
     9781 ccccaggcgc gccagcgcgc gcgcggcgcg agcgccgccg ccacgacgaa accggggaac
     9841 aggagcagcc cccagttgtg cgtgtagaag caggcgacga gggccagggc gtgggcgacc
     9901 agcgccccga tcgccccgtc gcgctggtac accaggaagc gctcggttgc gacgatcgcg
     9961 aagaggggca ggagcatgta catgcgcgcc tgctgcgaga actgcaccgc catcggcgtc
    10021 acggcgatga gccaggccgc tgcgaccgcg gcccgctccc cggccaggcg gtgcgccagg
    10081 accgccgtcg cggcgatgac gatggcgccg aggatgaccg acggcagccg gaccgccgcc
    10141 tcgctctccc ccatcacctg gatccagaga tgcaggagcc agtagtagat gggggggctg
    10201 gagtcgcgcg ccaggagcgc cgggatctcg gagaaggcgt gccgggcgat ggcgacgctg
    10261 ttcgcctcgt cgagccagag gtcgtgccgg gtgtagccgt gcaggcgcag gacatcgccg
    10321 aacagcaccg ccgcggcgat cgccagcagc gccgggagac cgatcgacct ggccgccggc
    10381 gcggccggcg tgctcgtcgc tggcagggga tccgaaacgg ttgtcaggcc ggcatgctcc
    10441 gagagacgcg ctcgcggcgg cgcggtggcg cggatcctac atcgggaatt gcgccgcaga
    10501 cccgggtcgg tccatgctac gcggccacgg gaggacacca ggcatgaaaa cgcggatcgt
    10561 ctcggtcgtc gtcgtgttcg tggtggccat gctgctcggg ttcgtggtgc acggcgtact
    10621 cttggcgaac gactacgcgc agctcacctc ggtgatgcgt ccgccagacg aggcgcagca
    10681 gaccttcccc tggatgatcc tggcgcacct cctcatcgcc gtcggctaca cctggatcta
    10741 cgtgcgcggg aaagagccga agccgtggct cgggcagggg gtccgcttcg gcctggcggt
    10801 ggcggtcctg tcggtgatcc cgatgtacct gatctaccac gccgtggcgc agttcccgct
    10861 gagcctggcg ctcaagcaga tcgtcttcga cacgatcggc tgcatcgtgc tcgggatcgt
    10921 cgtcgcgcgc gtcaatcggt gaccggcgcc gctgccgcgc gccgccgcgg agcgctacgg
    10981 ccgccgggcg gtgatggcga gcgccagcat cggggatcgc atcggacggt ctccgaaggc
    11041 gcgggcgaac tccaccgtca ggatcgggat gatctccgca ggggtggggg agccgcgctc
    11101 gacgatctgg ttgaagaccg ggctgccggc gatgaggccg gtggccgcgc tctccgccga
    11161 ggcggcttgc ccttcgatgc gcaccggcgt gatcgcgatc tcggtgaacc cggcctccga
    11221 caggagcgtc gtgagcgcct gcgggtcgtg atcgccgaac ggcgtcttca ggaagggcgg
    11281 cgggttgctc ggaaagagac tcgccagcac ctcgtgggtc agggcgctgg ccgggttgtc
    11341 gtggagcgat ccccaggtat tgagaaggta cgtccccccc ggcgtgagga cgcgccgcac
    11401 ctcgcgcacc gccttcggct tgtccggcat gaacatgagc ccgaactggc aggcgacggc
    11461 gtcgaacgac gcgtcgggga acggcagcgc catcgcgtcg gcctggcgga actcgatccc
    11521 gtcgatccct tcgagccgcc ggcgcgccac ctgcagcatc ccttcgttga ggtcggtcgc
    11581 gatcagcgtc gcggtgggcg gcagcctccg gcgcagccgc cgcgtcagga tgccggtgcc
    11641 ggcggcgatc tcgaggaccc ggaaagcgcc ggcccccgtc gggatcggca ggcgcgcggc
    11701 cagatcgtcg gcgtacggct cgaacagcag cgggcccagg tgcgcttcgt agagatccgg
    11761 gatcgacccg gcgaatttcg cgtcgctcga tgacatggcc tgctccctcg atgttgcggg
    11821 cggaacgacc gtcagcggcg gacgctcgcc ttgctctccg gcgccccgtc cgcctgcagc
    11881 gcggtgatgg tgacctcctc gatccgccag gcgacgatcg tccggtagac gtccctctgc
    11941 agcagcgcgc tctgggtgag gggccgcgcc ttgccggcgc ggaccggccc cgccaggacc
    12001 acctcgagca cctcgtactc gtcccggtcg agcgcctgca cgacgatctg caccgactcg
    12061 tccatgtcca gcgtgttctt caccatcacc ttgacgctca gctcgaggtc gccgtccgcg
    12121 ttcggcccgt cgagcacctt gtgctccggg gcggcgatct cgatgccgag gcgtggtgga
    12181 gccgcgatcg aggggacggc gagcatcagg acgaggagca tcagcaccac ggcgcgcatg
    12241 gcacctcccg aagggtcgcc gccatgatac ccacgaatca gggttcggac gccccggcgg
    12301 cggccgcgcc ggccggcttg cggacgaaca ggcgcttcag gaccggaatg agcgccagga
    12361 gtccgacgag aaggaacttc ttgaacgcca ggattccgag ccagagcgcc ttgaagagcc
    12421 ccgccttcac cgccgtcgcg ccgaggatga gcccggcgac gccgtaggcg gccgccttgt
    12481 cctttccggg gagatagtcg gcgtagcggt gcccctcgtt gaactccacc gccgccggca
    12541 gggccgcggc ctgctcgcgg acggtggcga gctgacccat cgacgcgacc gcgttgagga
    12601 ccagcacccc gcgtctcccg aggatgcgga tgttgtagtt cagcgtgtgc tggtcgctcc
    12661 cctcgaagtt caggtccttc gcccagtaga gcttgtgggt ggcggcgtcg tagtgcggcg
    12721 gctcggccca gccgacgagc gtcatcgacc cgaagccggc ttttttccgg cgttcgttct
    12781 ccgccttcat cccctcctgc atgtcctcga gcaggtcgtc gtagtcgatc gaggcggcat
    12841 ccttgtcgtc cacgtagccg tcctcgtcga agctgatcac gaccccccag ccgtcctcgg
    12901 agagcgggct cgtgtcgcgc ggcaccagca tcccgagcac cccctgggcc gcctgcgggg
    12961 ggttccccca cgcctccgtg aggagccggc ggcttccttc cggcggaatg aagcggaagg
    13021 tctcgggcag gtgcagggtg gcgagaccgt cctcgagcgt gatcgtgccg gtctggtagc
    13081 cgaggcccgc ttcgaactct tcggcgctca cgggcgaccc cccggacggc tcctgggcga
    13141 acgacggtgc ggcgacgctc atgatgacga cggcgaggac cggcagggga cggatcgtca
    13201 tgtctcctcc tccgtgatca gtggggacgg gccagcttga agtaggtgtg gatgacgcaa
    13261 tgtcccttcg aacaggcggg gaaaagggtc cgcccgccga agtcggcagt gcaggcgggt
    13321 gcggtgacga cctggatcgt cccgttccac tctcccgaga gatccgcgaa ggggccgggg
    13381 tcctcgcccg cggccggagg cggcgccacg agcagcagca ggctcgggag ggcggcggca
    13441 cgcacccgcc gcaggacctc ggacattgaa gcggagtcta tccgaaaccg ggcgcgaggt
    13501 cgtcgcccgc cgtgcgacgc ggcatactcg cggtcatgcc acgcaaacga atcgtcgcac
    13561 tcacgctcgc ggcgctgtcg ttcggcgccg ccccggcatt cgctcaatcg ctcaacgaac
    13621 ccgctctcat cgtgcagagc gtcgtctccg gcctcgagca gccgaccagt atggcgttcc
    13681 tcggaccgga cgacatcctg gtgctgcaga aggcggacgg tcgcgtccgg cgcgtcacgg
    13741 gcggggtcct ccaggccgcg ccggtcctcg acgtcgcggt cgattccctc agcgagcgcg
    13801 gtctcctcgg gatcgcggtc gatcccgatt tcgccgtcac gcggcacgtc tatctctact
    13861 acaccgaatc ctcgaccccc ctcgacacga cgggcgaggc gaaccctctc ggcaaccgga
    13921 tctaccagta cgactgggac ggatccgcgc tcgtcaaccc ggcgctcatc ctcgatctgc
    13981 cgtggtccac cggcccgaac cacgacggcg gcgtcatcgc cttcggcccc gacggcttcc
    14041 tgtacgccgt gatcggcgac ctcaaccgga gcggcaagct gcagaacatc cccctggggc
    14101 cggatcccga cgacacgggt gtcatcctgc gcatcgacac gaacggcctc ggagcccccg
    14161 acaatccgtt ctacacgccg gccgaaccgg cggcgcgcat gagccggtat ttcgcctacg
    14221 gcgtccggaa ttcgttcggg ctcgccttcg acgcgcgcac cggcgatctc tgggacacgg
    14281 agaatggccc gaacagctac gacgaggtga accgcgtcat ccccgggttc aacagcggct
    14341 ggaaccggat catggggccg gactcgcgtg acccgcaggg gatcggcgac ctgtgggtgg
    14401 ctccgggatc gatctaccgg gacccggagt tctcctggtt catcccggtc gcgccgacgg
    14461 cgctggtgtt ccccgaaagc ccgattgtcg ggtgcgcgct gcagcacgag ctgctcgtcg
    14521 gcgacagcaa ctgcgggcag atctaccgct tcaagctgaa cgcggcgcgc gacgcgctgg
    14581 cgctgagctc cgcgccgctg gtcgatcggg tcatggacaa cgccaccaac cgctgcctgg
    14641 acgaggtggg cgagatcgcg ttcggccagt cgttcggctc cgtcaccgat ttggagaacg
    14701 ggccggacgg gatgatctac gtggtgtcga tcacgacggg gcggatcttc cggatcgcgc
    14761 cgcgccccgg gttcttcccc gacgccgacg gggatggcgc cgacgacgcc tgcgactgcg
    14821 cggcgggcga cgccggcgcc tacgcggcgc ccggggagat cccgcggcag cggctcctcg
    14881 gcgcggcgcc gcacacggtc ttctgggaca gccggaccgg cgccttcggc cccggcacga
    14941 ccacgacgat cgtcagcggc ctcgcaggtg gtctgcgcgc gagcggaggc tacgccggcg
    15001 cctgcgatct ggcggtcggc gtcgcaggga cgtccttcgt cgatccgcgc gcggcgccgc
    15061 cggtcggcga ggcgtactac tacctcgtgc gggcgcagaa ctcctgcggg caggggacgt
    15121 tcggcgacgg cactccggtc cccgatccgc gcgatgagct cgacaccggc accaggcctt
    15181 cctgcggaag cccttagggc agtcgaatcg gcggcccggc ctcgggggga agcgaggcga
    15241 gggggaggcc gagcagctcc agcatcgtcg cggcgacatc cgcctggtgg accgtgggag
    15301 ccggtcccac cacgccgcga tcgggcgtgt cgggcccgat gatggcgatc cagatctcct
    15361 ccgacccggc gattccttcg ccgtggtcca cccagtcctt cggcgtccgg ccgcgtccgt
    15421 ggtcggtggt tacgacgagc gtcgtccggt cccgatagcc gtcgagcgac tgcagcgtct
    15481 cccacaggat ccgcatgtag tcgtcggtga gccgcaggca gtcgagcagc cggtcgtagc
    15541 ggcgcgcgtg cgcccagtcg tcggtctcgc cgagcgccag gtacagcacg cgcggccggc
    15601 gcgccttgag gtattcgagc gccaggtgga aggtcggcat gtcggtgcgg tacccctccc
    15661 acagcgccat gacgtgctcc tggtagtcgc cgagcgcgtc catcgccggg ttcgacaggg
    15721 cgggtggcac gcgcgcgtag ccggcgttgg cgaacaggtc gtccggttcc gaggaggcga
    15781 acagtccgag cgcgtcccag gtcgagaaga ccgcgacctg ctccgggcgc aggccgagcc
    15841 cgcggcggat cgcctgcagc acggtcggat agggatagcg gcgggcgtcg ttcgtcgtca
    15901 cgtcgggatg gaaccgaccg gtcagcagct cgaggtagcc cggcgccgag aatccgtgcg
    15961 ggttgcgcag cgtcgcccgg ctgccgcgcc ccgcgtctcc cagcaccacc ccctgcggcg
    16021 cgagcgtccc ccagaaatag ggccacagcg ccttccggcg cgcttcggca tcgtcccgcc
    16081 agtacaggcg ccgcgcccgc tccgggtcgt agatcccgga gcgcttgctg cggccgaagg
    16141 cgacgaccgg atccatgccg ccgaagactt cctggacgcg cactccgtcg agggtgagca
    16201 ggacgaggtt ctcggtccgg cggtccggca ccgccgaggc gatcgccagg ccgaatgcgg
    16261 cgaccgcggc ggcgagcgcg gcgcgccctg atgccaggtt tcccccgata cgactcatgc
    16321 aaacgcatca ttccgcggcc gttccccggg agtcaacgcg gggcgtatac cgcgccgcgc
    16381 aatgcgtacg cctttctccg accgggttct cgggggtatt tctccttgtc ggctctcggg
    16441 gatccgccta caaaagcgag cgattgatac atagcgccgc ggagtggctt cggcgcgggg
    16501 gcactggaac cgaccagggg gcacaggagc accgtcagag gaggccccca tgagggcgtg
    16561 cgtagtggtc gtcgcgctgg tgatcagcgc gttgctttgt acggcagttc acggtcagga
    16621 aaccactagc gggacgatcg ccggcaaagt catcgacccc gccggaaaac cgatcgccgg
    16681 cgccatcatc atcgcgcagt ctgattcggg tccgcgcacc gccaccaccg acgacgcggg
    16741 gaactacgtc ctgccgttcc tccgtcccgg cacctacacg gtgcgtgtcg aggccccctc
    16801 cgggttcaac accgccctgc aggaaggggt gatcgtcggg ctcaaccaga gggtcccgct
    16861 cgacttcacg ctgacccccg ggaaggtgga ggagatcacc gtcacatcca agccgctgat
    16921 cgactccacc tcgaccgcca gcggcaccaa catccgctac gacgagttcg tcaactcggt
    16981 cccgatcgga cgctcgttca ccgacaccta cggagtcgcg agcggcgtcg tcagcggtcg
    17041 cggcaccggt caggggaact actccatcag cggcgcgtcg gggctggaga actcctacct
    17101 catcgatggc gtcaacgtca ccaataccgg ctacggcggg atcggcgcct acaacatcgt
    17161 ctacggatcg ctcggcaccg gagtcaccag cgagttcctg gacgaggtgc agatcaagac
    17221 cggcggcttc gaggcggagt acggccaggc gctcggcggc atcatcaaca ccatcgtcaa
    17281 gtcggggacc aacgacttca aggggagcgt cgcctggtac gcgacaccgc agggggcgac
    17341 gtccgactac accgtgaccg agctgcaggc gggcgccgcg agcaccgtgg agcagggggt
    17401 caacgacttc gccttccagg tcggcggccc catcgtgaag gacaaggtct tctatttcat
    17461 cgcctacaac ccggtgatca cgacgacggg ccgggaggcg caaacgctcg cgaacccgat
    17521 gttcgccccg gcggaggccg gcgtgtccgt cttcgacgag acggctccga ccggcttcgg
    17581 tgtgccggcg gcgctggcct tcccgtccgc cggccggacc ctggagcggc ggcgccaggc
    17641 caacaactac gcggccaaga tcagcttcca gatgaccgag aaccagaacc tcgagctgac
    17701 cgccttcggc gacccggcgg ccggcgacat ggggccacag cgcgacatcg cgccgctgtt
    17761 cggcgacttc gcggacggcg gcggggagag cagcgtgcgc tacgggtcca acaacctggc
    17821 gctcaagtgg aacgccatcg tcacgccgcg tttcttcctg gaggggatcc tggcgcacca
    17881 tgacgggttc ttccgcgaga cgacggggct gaaccagtac cgctacatcg actaccggaa
    17941 caacctggag ttccgccggg gcgcgaccac ctacaccgat ccggtcctcg ggccgacgcc
    18001 gttcaccgca tcggcgacgc tgccgaccga ggggggcgtc gggttcatca cgtcgcagga
    18061 cgaccagcag acccaggcga tcctcaagct gacgaacacc ttcgagcagc acgaggtgaa
    18121 atacggcgtg gagtacgaca cgatccgcta ccgcgagaac gccgcctaca ccggcccctc
    18181 gttcaatatc cagctgccgg tgtcgttcta cgatccggcc acgctgacgg tgtcgccggt
    18241 ggacgccgat tcggacggaa cgcaggactt cctgccgatc gcctcgcgcg gaggggcgat
    18301 cgtccaggtg cgcaacggcg ccggcgccga cccgacggtg gcgttcgaca gcgccaacaa
    18361 cttccgcgtc acccgggccc gcatgggacc cgagctcccg tacaccaacg cgaacgagtg
    18421 gaacggcttc gtccaggaca cctggtcgat cacctcgcgc gtctcgctca gggccggagt
    18481 gcgctacacg caggagacga tcgacggcgc cggcacgttc acgctgccgt tcgcgacctc
    18541 gacggtggag caggaggtga cccccggcaa caccgtcgag agccgcatct tcacgcccgg
    18601 gacctcgacg tacacgccga actcctacac cttcgccggc aactgggcgc cgcgcctcgg
    18661 cgtctcgtgg gatgttctgg gcagcggccg cagccgcctg tacgtgaacg tcgcccgcta
    18721 cttccagcgg gtcccgagcg acctcgcggt gcgcgccttc agcaacgagg tcggcatcag
    18781 cctgcaggag ttcaacacgc gcgacctgag cgtgccgcgc accgccggga ccgacggatg
    18841 cgtcgacaac ggcggcaccg cgaccaccac ctgcgcgacg tcgttcccgg tgttcacgca
    18901 gggggtcgat cagaccaacg tcgtcggcgg cgtgaagctg ccgtacgagg acgagatctc
    18961 cggcggctac gccttcgagc tcaacgactc gtcgtccctc gaggtgcggg cgatcttccg
    19021 cgaacagggg cgcgtcctcg aggacacgca ggtcaatgcc atcgagcaga tccagaactt
    19081 ctactacggc accgcctacg gctatccgta cgatccgttc ggcggctcgc cggccacccc
    19141 ggcgagcacg accttcccgg cccgcacctt cggcacctac tccctcgcga acccgggaac
    19201 gccccagatc ccgcaggggc tggcgccggg gatcaacttc ccgaagccgg agcgcaccta
    19261 caaggcgctc gaggcgatct acaccaagcg gttcgatccg cactggtcgc tgttcgccaa
    19321 ctaccgcctg gcgcggctgg aggggaacta cgaggggctg ttccgcaacg acaacggaca
    19381 gagcgatccg aacatcacgt cgctctatga cttcccgagt tcgccgttga tgcaggggca
    19441 gttcatcaag gggccgctgc cagccgacgc cacccacgtg gtgcacatct tcccgtcgta
    19501 cgtgttcgac atgaagctgc gggtgggggc gaacgtgtcg tggtcctcgg gcttcccgcg
    19561 gacgtccatg ctggcgcatc cgatctacca gaacgccggc gagatccccg ggatcgaccc
    19621 ggtctacgcc tactggcagg acgacgggac cggaacgctg gccctcgcca cgacgagcga
    19681 cctgtcgagc gcgctggccg atccgaacgc catgagcaat atcttcctga ggtcctacac
    19741 gccggtgaag cgcggcaatc tcggccggct gccggacctc tggactctcg acctgcacgc
    19801 cgactacccg atcgcggtcg gcaagacgag cctcgcggtg atgttcgacg tcttcaacgt
    19861 caccgacgag gacacggtga cgaacttcaa cgacaacgtc gagctcaccg ccggcgtcac
    19921 cgatccggac ttcacggcgc cgctgaccta ccaggcgccg cggacggtgc gccttgcggc
    19981 gcgctggtcg ttctagacgg actccctcgc ggaggccttc gggcctccgc cctcgccccc
    20041 tgcggcggtc ggcatgcccc gcaggggttc gtcttttcgt ccgggggccc ggacaggctc
    20101 ccccgttggc gcgctgatac catagggcct ccattccgga ggagcgcggg tgcccgacga
    20161 gaagtcgatt gccgatgcct tcagacgctg gggatacctg gccgctgatc tcgattcgct
    20221 gggacggctg ccgccgttca cgcatccgga aatcctggac gtcgcgcagg gaaagacggc
    20281 cgccgcggcg gagcgctacc gcaagatcta ctgcgggccg atcggcgccg agttcatgca
    20341 cctgcgccgc ggcgaggtgt ccgactgggt cgccgcccgg atggagcggg agccgcgggc
    20401 gccggaccgc ccggcgatcc tccgccggct ggcgcaggcg gagctgctcg agcagttcat
    20461 gcaccggcgc tgggtcggga gcaagcgcta ctccctcgag ggatccgcct cggtcctggt
    20521 ggcgctcgac aacatcctcg acgaagcggc ctcggccgag gtcgcgctca tcgccatgag
    20581 ccatcgcggc cgcctcaacg tcgtggtcaa catcgtcggc cgcgcccccg gcgatgtctt
    20641 cgcccggttc gaggaggggg atccgcgcgc cttcctgggc ggcggcgacg tgcgctacca
    20701 cctcggggcg accggcgagt accggaccgt ttcggaccgc atcatccgac tgcacctggt
    20761 gtccaacccc agccacctgg aggcggtcga cccggtgatg atggg
//
LOCUS       DASRIQ010000545         8370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3089267, whole genome shotgun sequence.
ACCESSION   DASRIQ010000545 DASRIQ010000000
VERSION     DASRIQ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089267"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFD06_13480"
     CDS             complement(<1..737)
                     /locus_tag="VFD06_13480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN04589.1"
                     /translation="MAETTVKPKPLRGWRAIVAAMKSPNTAAVSLLAFASGLPLGLVW
                     IAIPDWLRSSGADIKTVGLVTLAQAPWTFKILWAPLMDRWAPPWLGRRRGWAAIAQVA
                     LMILTLALAGVGHRPETPWVVLALALAIAVASATQDIAIDAYSVDVLKPEEQGVAVGA
                     SRGIYRVGMFMAGAWSITLAGWWSWPVVCVILALLYLPMLVITLRAPEPADRIVPPKS
                     IRDAVWLPFLGFLGRARALEILAFVFC"
     gene            complement(795..2357)
                     /locus_tag="VFD06_13485"
     CDS             complement(795..2357)
                     /locus_tag="VFD06_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZN04590.1"
                     /translation="MAGDDKTGGKPADDKAGGKPDDNRNEKTGDARLEQKPGDSKPEK
                     NGRRNGAAPTATAATTEAPPEAPATEAPAPATAEGPATATAVLTEPTKEPRQIRFLFI
                     SKWGLIHDLAWEVRKEGHEVRYHIMSKADREVGDGFVDKVDRWEEHKDWADVIVFDDC
                     EFGTLPDRLRKEGKAVVGGTPYSDKLEMDRDFGQEEMKAAGLTVLPRWEFDSFDAAVS
                     FIKVNPGRYVIKPSGKAQNEKVLSFVGQEEDGLDVLTMLEHYKNGWASKIKTFQLQKY
                     ASGVEVAIGAFFNGKEFLQPVFVNFEHKRMFNDDIGPQTGEMGTAGFWYGPCTLFTQT
                     LQKMKDRLADAGYVGYMDINCIANNRGIFPLEFTCRFGYPTINLQLEGVLSRWGEFLA
                     ALARGQTFNLRTKKGFQVCVVIAVPPFPFVDPDAFKKFSEDAAVIFKKPMNEGIHPCD
                     IRLVEGDWRLAGNSGYALVVSGSGSTMDDARREAYNRVRNIIIPNMFYRTDIGERWIR
                     DGDLLQTWGYMA"
     gene            complement(2371..3639)
                     /locus_tag="VFD06_13490"
     CDS             complement(2371..3639)
                     /locus_tag="VFD06_13490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN04591.1"
                     /translation="MSPASPPAPGDGAAGAPLFGGPLPAAALVLAMITEAIGYGMVAP
                     TLPFMARLTGVGEDQIGLLVGIYAAVGLVAAPWLGTLANRRGHRFIILIGLACLTVAS
                     LAFTVAPNYPSLVLARLVQGFGAAGVWVGSLTLAAELSADATMGRSLSWLTGAWSLGF
                     IVGPALGGVGTLRTPFLVYALLAACAFVIGIVGLPHTGGGRPAATLAGILRIARRPSI
                     LASGAATFGLAFYYGAIEAFVPLLIAAGGDPHAAGGKETAAGVVVAGRMGIGLLFSIA
                     GLPSVLLPRVVGRLADRYGDAPIIGAGFLLAAASSASFLSLYGRAPDAVVFLLLGAVE
                     VLVYVPGVALLHRGVASEDRVYASGSHAYAFSIGFFLGPLVTGVLFPLGGFRTLFTVL
                     VLSSLLSAAAVALLSRRAARPPLADRSSAY"
     gene            complement(3636..4979)
                     /locus_tag="VFD06_13495"
     CDS             complement(3636..4979)
                     /locus_tag="VFD06_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN04592.1"
                     /translation="MPNTETPKSSGAAAVAPKGQEMLSAAEKHKRYLFPCTATYYETP
                     LTIVRGEGMHVWDETGRKYLDCFGGVLTVNVGHCHPEVTAAIVKQAQTLVHTSTLYVT
                     PPVGDLAEAMAQALPGELNRLFMTNSGSEADETAILLARVHTGLQEIVVLRYGYSGRT
                     MMAMASSGQAPWRKLPQPVPGFVHALAPYCYRCPLKLTYPSCEVACAKDVKEVIETTT
                     TGRIAGVMVEVIMGSGGFLVPPKEYFPMVADIVRKAGGVFIADEVQTGFGRTGKLWGV
                     DHYGITPDIMTFAKGMANGTPVGATATTETIAASLKPLSFSTFGGNPVTSVAALATLR
                     VIQKQNLAENARVMGERLHQGFLELQRKHKVIGDVRGIGLMRAIELVGEGKAPDAPTT
                     ARVLEGARKRGVLVGKGGLWANVLRVAPPLIVNAAQIDELVAALDGAFTDATANP"
     gene            complement(5015..6646)
                     /locus_tag="VFD06_13500"
     CDS             complement(5015..6646)
                     /locus_tag="VFD06_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN04593.1"
                     /translation="MDRRDFIRIVVTGSLAGMACGRGGPERSADGAAAPSRSDLTPVE
                     TRETNAVCHALRDGAAVRLPKPRRHVPIVIVGGGPAGLCAARFLDDRPYLLIEKEEQV
                     GGNATGGFWRGVGFSTGASYNNNRDVKALAAELNVPLYPIRSVDGMIVRDIYVPEFFT
                     EGMRRAPYPQRVRDAFRRFVDTYHSYDPEKELERLDNLPFAEILKDYPEEVTAFFDSF
                     GPNNWGAPVADTSAFIGIEAAQWMGGLEPERYAGEAGFGPLAQAVGASVAARGPERLL
                     LGATVARVEAQGDRILVAYVPPGADLDAAAGAASGGSANASGGTPSSAASIWEGPPLP
                     LECVSADTVIVAAPKFIARHLVQGLPPEQQKAMQSIRYEPYMVTNLCFDGVVHEGCFD
                     TNIPAPDVMTDFVCADFPKTHGQGDPKRPTVLSCYMPQRREDRALLLEESEVRASALQ
                     ALDRIDRWFPGAAGRCREIQVRLRGHPMHASMCGMITRTGPLSRQSLGAIHFAGTDGV
                     GGVSEFAAALESGREAAGRAIASLDAATRRHHRLG"
     gene            complement(6646..>8370)
                     /locus_tag="VFD06_13505"
     CDS             complement(6646..>8370)
                     /locus_tag="VFD06_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN04594.1"
                     /translation="GESILPFCYGGSNGLLTQDAADARLFRGLGASRLLRTVCAEPTG
                     FVARVLYGKMPIVDFPDFEAARFILVWGANPRESNIHLVPYLKAAKAQGARIAIVDPR
                     RIAGEGMVDLHLPVWPGSDAAVALAMIGHLDREGRVDRGFLEKHATGWELLLDAARPW
                     TLERAAAMARVEPHAIAALADAYADADPALVRCGWGVERNRNGEAAVAAILALPAVAG
                     KFGKRGGGYCLSASGSYRVDHDAVAGAREPNTRPVNMNRLGRVLLEETSPPIRALFVY
                     NANPAATIPDQGRVLEGLRRDDLFTVVFDQVMTDTALYADILLPATTFLEHTDLSTSY
                     GTYSVMLGEPVVAPAGESKSNEEVFTLLGRALGLDPEHRWVTGETQVTQALAAIRGPL
                     EGTDGADRLARLRERKVLRMDFPGEAPVQFGNVFPNTSDRKIHLWPDAEWARPYEVLP
                     DPEERPGRGRHPLALISPATDRSISSTLAEYGFKEAVVMLHPEDAAKRSLKDGGLVRV
                     HNDLGEVVVRLRVSDEVRPGVASLPKGIWNRHTRNGRVGNTLVPDDVSSASGGACFND
                     ARVEVAPA"
BASE COUNT         1252 a         2885 c         3014 g         1219 t
ORIGIN      
        1 tagcagaaga cgaacgccag gatctcgagg gcgcgcgcgc ggccgaggaa tcccaggaac
       61 ggcagccaga cggcgtcgcg gatcgacttc ggggggacga tccgatcggc cggctcgggg
      121 gcacggaggg tgatgaccag catcggaagg tagagcagcg cgaggatcac gcacaccacc
      181 ggccacgacc accatccggc gagggtgatg ctccaggccc cggccatgaa catgccgacc
      241 cggtagatgc cgcggctggc gccgaccgcg accccctgct cttccggctt caggacgtcg
      301 acgctgtagg cgtcgatggc gatgtcctgc gtcgccgatg cgacggcgat ggcgagcgcg
      361 agggccagca ccacccacgg cgtctcgggg cggtggccga cgccggcgag cgccagcgtc
      421 aggatcatca gcgcgacctg cgcgatcgcc gcccatccac ggcggcggcc gagccacggc
      481 ggcgcccagc gatccatgag cggcgcccag aggatcttga aggtccacgg cgcctgcgcc
      541 agggtgacga ggccgaccgt cttgatgtcg gccccgctgc tgcggagcca gtccgggatg
      601 gcgatccaga cgaggccgag cggcaggccg gaggcgaagg cgaggagcga caccgccgcc
      661 gtgttcggcg atttcatcgc cgcgacgatg gcgcgccagc cgcgcaacgg cttcggcttc
      721 actgtcgtct cggccatgcc ggtattaata gaggatggga ttgcggcggg gcaaccgcct
      781 gacggaaacg taactcacgc catgtagccc caggtctgca gcaggtcccc gtcgcggatc
      841 cagcgctcgc cgatgtcggt gcggtagaac atgttcggaa tgatgatgtt gcgcacccgg
      901 ttgtaggcct cgcggcgggc gtcgtccatc gtcgagccgg agccgctcac gacgagcgcg
      961 tagccggagt tccccgccag gcgccagtcc ccctccacga ggcggatgtc gcaggggtgg
     1021 atcccctcgt tcatcggctt cttgaagatg accgccgcat cctcgctgaa cttcttgaac
     1081 gcgtcggggt ccacgaacgg gaacggcggc accgcgatga cgacgcagac ctggaaccct
     1141 ttcttcgtgc gcagattgaa ggtctggccg cgcgccaggg ccgcgaggaa ctccccccag
     1201 cgcgacagca ccccttcgag ctggaggttg atcgtcgggt agccgaagcg gcaggtaaat
     1261 tccaaaggaa agatgccccg gttgttggcg atgcagttga tgtccatgta gccgacgtag
     1321 ccggcgtcgg cgaggcggtc cttcatcttc tggagcgtct gcgtgaagag cgtgcagggg
     1381 ccgtaccaga acccggccgt ccccatctcc cccgtctgcg ggccgatgtc gtcgttgaac
     1441 atccgcttgt gctcgaaatt cacgaacacc ggctggagga actccttgcc gttgaagaag
     1501 gcgccgatgg cgacttccac tcccgacgcg tacttctgca gctggaacgt cttgatcttc
     1561 gaggcccagc cgttcttgta gtgctcgagc atggtgagga cgtcgagccc gtcctcttcc
     1621 tggccgacga acgacaggac cttctcgttc tgggccttcc cggacggctt gatgacgtag
     1681 cgccccgggt tcaccttgat gaacgacacg gcggcgtcga acgagtcgaa ctcccagcgc
     1741 ggtagcacgg tgagcccagc cgccttcatc tcctcctggc cgaagtcgcg gtccatctcc
     1801 agcttgtcgc tgtacggcgt gccgccgacg accgccttcc cttccttgcg cagcctgtcc
     1861 gggagggtgc cgaattcgca gtcgtcgaag acgatgacgt cggcccagtc cttgtgctcc
     1921 tcccacctgt cgaccttgtc gacgaagccg tcgccgacct cgcggtccgc cttcgacatg
     1981 atgtggtagc ggacctcgtg cccctccttc ctcacctccc aggcgaggtc gtggatgagc
     2041 ccccacttgg agatgaacag gaagcggatc tgccgcggct ccttggtcgg ctcggtgagg
     2101 acggcggtgg cggtcgcggg accttcggcg gtggctgggg cgggcgcttc ggtcgcgggg
     2161 gcttcggggg gagcctcggt cgtcgctgcc gtggcggtcg gcgcggcgcc gttccggcgt
     2221 ccgttctttt ccggcttgct gtcgccaggc ttctgttcga gccgcgcgtc gcccgtcttc
     2281 tcgttcctgt tgtcgtccgg ctttccgccg gccttgtcgt cggccggctt gccgccggtc
     2341 ttgtcgtcgc ccgccatcag cgggcgcaat ttaatacgcg gaagagcgat cggcaagggg
     2401 cgggcgcgcc gcgcggcgcg aaaggagcgc gacggcggcg gccgagagga gcgacgagag
     2461 caccagcacg gtgaacaggg tgcggaatcc gccgagcgga aaaaggacgc cggtcacgag
     2521 cgggccgagg aagaagccga tggagaaggc gtaggcgtgc gagccgctgg cgtagacccg
     2581 gtcctcggac gcgacccctc ggtgcagcag ggccaccccc gggacgtaca ccagcacctc
     2641 gaccgccccg agcaggagga acaccacggc gtccggggcg cgcccgtaga gcgaaaggaa
     2701 cgaggcgctc gaggcggccg cgaggaggaa cccggcgccg atgatcggcg cgtcgccgta
     2761 gcggtcggcg agccgtccga cgacgcgcgg caggagcacg ctcggcagcc cggcgatcga
     2821 gaagagaagc ccgatcccca tccgccccgc gaccacgacc cctgccgccg tctccttgcc
     2881 acccgccgcg tgcgggtcgc cgccggcggc gatcaggagc ggcacgaacg cctcgatggc
     2941 gccgtagtag aaagccaggc cgaacgtggc ggctcccgag gcgaggatcg acggtcgccg
     3001 ggcgatccgg aggatgccgg ccagggtcgc cgccggccgg cccccgccgg tgtgcggcag
     3061 cccgacgata ccgatgacga acgcgcaggc ggcgaggagg gcgtagacga ggaacggggt
     3121 gcgcagcgtg ccgacgccgc cgagcgccgg gccgacgatg aagccgagcg accaggcgcc
     3181 ggtcagccac gacagcgacc gccccatggt ggcgtcggcg ctcagctcgg cggcgagcgt
     3241 caggctgccg acccagacgc cggccgcgcc gaacccctgc accagccgcg ccaggacgag
     3301 cgacggatag ttgggggcga ccgtgaacgc cagcgacgcc accgtcagac aggcgagccc
     3361 gatgaggatg atgaagcggt ggccgcggcg gttggcgagg gtcccgagcc atggcgcggc
     3421 gaccaggccc acggcggcgt agatcccgac gagcagcccg atctggtcct cgccgacgcc
     3481 ggtgaggcgg gccatgaacg gcagcgtcgg cgccaccatg ccgtagccga tcgcctcggt
     3541 gatcatcgcg aggacgaggg cagcggccgg cagggggccg ccgaacagcg gcgcgcccgc
     3601 ggcgccgtct cccggcgccg gaggcgacgc cgggctcaag ggtttgcggt cgcgtcggtg
     3661 aaggcgccgt cgagggcggc gaccagctcg tcgatctgcg cggcgttgac gatgagcggc
     3721 ggggcgaccc ggaggacgtt cgcccagagg ccccccttgc cgacgaggac gccacgcttg
     3781 cgcgcccctt cgaggacgcg ggcggtcgtc ggcgcgtccg gggccttgcc ctctccgacc
     3841 agctcgatcg cccgcatcag gccgatgccg cggacatcgc cgatcacctt gtgcttgcgc
     3901 tgcagctcga ggaagccctg gtgcagccgc tcccccatga cccgcgcgtt ctcggcgagg
     3961 ttctgcttct ggatgacgcg cagcgtcgcg agggcggcga cggaggtgac cgggttgccc
     4021 ccgaaggtcg agaaggacag gggcttgagc gacgcggcga tcgtctcggt cgtggcggtg
     4081 gcgccgaccg gcgtcccgtt cgccatcccc ttggcgaacg tcatgatgtc cggggtgatg
     4141 ccgtaatggt ccacgcccca gagcttgccg gtgcggccga agccggtctg cacctcgtcg
     4201 gcgatgaaga cgccgccggc cttgcggacg atgtcggcga ccatcgggaa gtactccttc
     4261 ggcggcacga ggaacccgcc ggatcccatg atcacctcca ccatgacgcc ggcgatgcgg
     4321 ccggtggtcg tcgtctcgat cacttccttg acgtccttgg cgcaggccac ctcgcacgac
     4381 gggtacgtca gcttcagcgg gcagcggtag cagtacggcg ccagcgcgtg cacgaagccg
     4441 gggaccggct gcgggagctt cctccacggc gcctggccgc tcgaggccat cgccatcatg
     4501 gtgcgccccg agtagccgta gcgcaggacg acgatctcct gcaagcccgt gtggacgcgc
     4561 gccagcagga tcgcggtctc gtccgcctcg ctcccggagt tggtcatgaa gagacggttg
     4621 agctcgcccg ggagcgcctg cgccatcgcc tcggcgagat caccgaccgg cggcgtgacg
     4681 taaagcgtcg aggtgtgcac gagggtctgc gcctgcttga cgatggcggc ggtgacctcg
     4741 ggatggcagt gaccgacatt caccgtcagg acgccgccga agcagtcgag gtatttccgg
     4801 ccggtctcgt cccagacgtg catcccctcg ccgcgcacga tggtcagcgg ggtctcgtag
     4861 tacgtcgccg tgcaggggaa gaggtaccgc ttgtgcttct cggcggcgct cagcatctcc
     4921 tggcctttgg gggcgacggc ggcggccccg gaggacttcg gcgtctcagt gttcggcatg
     4981 ggtcaccttt gtcggcgcat gggttgaacg catcttagcc tagacgatgg tgcctgcgcg
     5041 tggcggcgtc aagggacgcg atcgctcgtc cggccgcctc ccggccgctt tcgagggccg
     5101 cggcgaactc gctcaccccg ccgaccccgt cggtgccggc gaagtggatc gcgccgaggg
     5161 attgccgcga caacgggccg gtgcgggtga tcatcccgca catcgaggcg tgcatcgggt
     5221 gtccgcgcag ccgcacctgg atctcgcggc agcgtccggc ggcgccgggg aaccaccgat
     5281 cgatccggtc gagcgcctgg agcgcactgg cgcggacctc ggattcctcg agaagcagcg
     5341 cccggtcctc gcggcgctgc ggcatgtagc acgacaggac ggtcgggcgc ttcggatccc
     5401 cctggccgtg ggtcttcgga aagtcggcgc agacgaagtc ggtcatcacg tcgggggcgg
     5461 ggatgttggt gtcgaagcac ccttcgtgca cgaccccgtc gaagcagagg ttggtgacca
     5521 tgtacggctc gtagcggatg ctctgcatcg ccttctgctg ctccggcggc aggccttgga
     5581 ccaggtgccg cgcgatgaac ttcggcgcgg cgacgatgac ggtgtcggcg gagacgcact
     5641 cgagcggcag cggcggcccc tcccagatcg aggccgcgga agacggcgtg ccgccggacg
     5701 cgttcgcgga gccgccggac gccgcgccag cggccgcgtc caggtccgca ccggggggga
     5761 cgtaggcgac caggatccgg tccccctgcg cctcgacgcg cgccaccgtc gcgcccaaaa
     5821 ggagccgctc cgggccgcgc gcggcgaccg aggcgccgac cgcctgcgcc agcgggccga
     5881 agccggcctc gccggcgtag cgctccggct ccagccctcc catccactgc gccgcctcga
     5941 tgccgatgaa ggcggaggtg tcggcgaccg gcgcccccca gttgttcggg ccgaacgagt
     6001 cgaagaaggc ggtgacctcc tccgggtagt ccttgaggat ctcggcgaac ggcaggttgt
     6061 cgagccgctc cagctccttc tcggggtcgt aggagtggta cgtgtccacg aagcgccgga
     6121 aggcgtcgcg cacccgctgc ggataggggg cgcgccgcat cccttcggtg aagaactccg
     6181 gcacatagat gtcgcgcacg atcatcccgt ccacgctccg gatgggatag agcggcacgt
     6241 tcagctcggc ggcgagcgcc ttcacgtcgc ggttgttgtt gtaggaggcg ccggtgctga
     6301 agccgacgcc gcgccagaag ccgccggtcg cgttgccgcc gacctgctct tctttttcga
     6361 tcagcaggta cggccggtcg tcgagaaagc gcgcggcgca caggccggcg gggccgccgc
     6421 cgacgatgac gatcggcacg tggcgcctcg gcttcggcag gcgtaccgcc gcgccgtcgc
     6481 gcaaggcgtg gcagacggcg ttggtctcgc gggtttcgac gggggtcagg tcggatcgcg
     6541 acggtgccgc ggctccgtcc gcgctgcgtt ccggaccgcc gcggccgcag gccatgccgg
     6601 cgaggctgcc ggtgacgacg atccggatga agtcgcggcg atccactacg ccggggccac
     6661 ttcaacgcgg gcatcgttga agcaggcgcc gcccgacgcg gacgagacgt cgtccggcac
     6721 cagggtgttg ccgacccggc cgttcctcgt gtggcggttc cagattccct tcggcaggct
     6781 cgccaccccg ggacggacct cgtcgctgac gcgcagccgc acgaccactt cgccgagatc
     6841 gttgtggacg cgcacgagcc cgccgtcttt caggctccgc ttcgcggcat cctccggatg
     6901 gagcatgacc accgcctcct tgaagccgta ctcggcgagg gtcgaggaga tgctgcggtc
     6961 ggtggccggc gaaatcaggg cgagcggatg gcgccccctg cccggccgct cctcggggtc
     7021 cggcagcacc tcgtacgggc gcgcccactc cgcgtcgggc cagaggtgaa tcttccgatc
     7081 ggaggtgttc gggaagacgt tgccgaactg caccggcgcc tcgccgggga aatccatcct
     7141 caggaccttg cgctcgcgca gccgggccag acggtcggcg ccgtccgtcc cttcgagcgg
     7201 gccgcggatc gccgcgagcg cctgcgtcac ctgcgtctcg ccggtcaccc agcggtgctc
     7261 cggatcgaga ccgagcgccc gcccgaggag ggtgaacacc tcctcgttgc tcttcgactc
     7321 ccccgccggg gccaccaccg gctcccccag catgacgctg tatgtcccgt acgaggtgct
     7381 gaggtcggtg tgctcgagga aggtggtcgc cggcaacagg atgtcggcgt agagcgccgt
     7441 gtcggtcatc acctgatcga agacgacggt gaagaggtcg tcacgacgga gtccttcgag
     7501 aacccgcccc tgatccggga tggtcgccgc cgggttggcg ttgtagacga acagcgcccg
     7561 gatcggcggc gaggtctcct cgagcaggac gcggccgagg cggttcatgt tgaccggccg
     7621 cgtgttcggc tcgcgggcgc cggcgacggc gtcgtgatca acccggtacg agccggaggc
     7681 ggagaggcag tagccgcctc cccgcttgcc gaacttcccg gcgacggccg gcagggcgag
     7741 aatcgccgcg accgccgcct cgccgttgcg gttgcgctcc accccccagc cgcagcgcac
     7801 gagcgccggg tcggcatccg cgtacgcgtc cgcgagggcc gcgatggcgt gcggctcgac
     7861 ccgcgccatc gccgcggcgc gctccagcgt ccacgggcgc gccgcgtcca ggaggagctc
     7921 ccagccggtg gcgtgcttct cgagaaagcc gcggtcgacg cgcccttcgc gatcgaggtg
     7981 gccgatcatc gccagcgcca ccgccgcgtc ggagccgggc cagaccggca ggtgcagatc
     8041 gaccatcccc tccccggcga tgcggcgcgg gtcgacgatg gcgatgcgcg ccccctgcgc
     8101 cttcgccgcc ttgagatagg ggacgaggtg gatgttgctc tcgcgcgggt tcgcacccca
     8161 gaccaggatg aagcgggccg cctcgaagtc gggaaagtcg acgatcggca tcttgccgta
     8221 gagcacgcgc gccacgaagc ctgtcggctc ggcgcagacg gtgcgcagca gacgcgaggc
     8281 ccccagcccc cggaagaggc gcgcgtcggc ggcgtcctgc gtcaggaggc cgttcgagcc
     8341 gccgtagcag aacggcagga tcgactcgcc
//
LOCUS       DASRIQ010000546        10527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_458678, whole genome shotgun sequence.
ACCESSION   DASRIQ010000546 DASRIQ010000000
VERSION     DASRIQ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10527
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458678"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /gene="purH"
                     /locus_tag="VFD06_13510"
     CDS             <1..463
                     /gene="purH"
                     /locus_tag="VFD06_13510"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZN04595.1"
                     /translation="DGALDLRSISGGLLAQDGDTGRADVPAARVVTRRAPTDEERTAL
                     AFAWRVAKHVKSNAIVLARDEHTVGVGAGQMSRVDSVRLAIAKAQEPVRGTSLASDAF
                     FPFRDGVDEAAKAGVTAVVQPGGSVRDEEIVAAADEHGMAMLVTGMRHFRH"
     gene            complement(503..3067)
                     /locus_tag="VFD06_13515"
     CDS             complement(503..3067)
                     /locus_tag="VFD06_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04596.1"
                     /translation="MIDDELLQELLVRATGLYNNGDYRGAIAAWREALAVDPSSQKAR
                     EGIQMATLLLADWDPTVAPAETEPARPADGAGAGPSASPEADAQRIDAGVKRVKSLLL
                     ERKYSEAIEGARGLVPIDPDSEEVQRLVEEAQQAFESAPFIDEHLTLSQELLDQGRRD
                     EAEAECRKVFALDPANPHAHAFLVKIQGKGAASPAPAAPEPAGDLGGRTMRIDRSQLQ
                     AFQVSKPSPPAAAGPPAAAAAAEAPRETAEESREAEGADETPFDVEPSFGTEPPETDR
                     IEIADPSADTGAPPQEEAGETAGAGEPELDFLAGDSLDLGADASAIPDVEVPEIEIEG
                     LSLDAPLEVAAPEADAAGPETEAAGPEQETAPAAVEAPGEPEVIEATTVVPPSVRLVE
                     RSAEGQAALDRLMQQLDEEPEAIESIPLAVPGAPPVTATDPGAPARAAAPASAQARPD
                     AGWEQELESLNQKSGAHEIVGRSAARASAPEAPADEDLDLTTLMGDEIGPLQAPAHDS
                     QEGPPAEIPPPAPAPPPVPDLAFAIPTVRPGPRQRERSAARLASMSPAGERRASATTS
                     LFAICGVVILAVAAVVWWFFFQPRNADGRSQAPTAAAPPSASSAADAADPGPIPTPIG
                     STSKQTEPIGHQEGTGSDGPSPAPSVEETAPVAVAPSGAAPGTAKSAVAPAPAPARPR
                     TAEEIRQETAQHLAAGKAFLEEERWQEARRELAAALALDPINFECKELLGKAQAKVDQ
                     ETKVRQDYDEARRLFQDKDYQGALWKLYRLPKDPRYGDLDVPIRNAWYNWAVVALKGG
                     DATTATQKLNEVFQVDPADAEAEKMMAVATQYASRPKDKGFYSYVDTLRVRSFDRK"
     gene            complement(3170..3841)
                     /locus_tag="VFD06_13520"
     CDS             complement(3170..3841)
                     /locus_tag="VFD06_13520"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04597.1"
                     /translation="MTREQWRFLVSGILFGFLVGYVVAWGVAVPRVKHEAAPVPEAGN
                     LGMTGAVIGPGAGSGNSGGGEAGTEAQGQEGGAGREELMARVFQEIASLREALEKNPR
                     DVQALVRLANFYQDAGKYDQAIEYYRRALAEDGKLVDARTDLGICLREMGRPDDAIAE
                     FRRSLDTDPRHWQTWLNLGIVSLFDKHDLETAAAAFGKVEELHPEYPGLAELKEEVRR
                     ARNPA"
     gene            3952..4866
                     /locus_tag="VFD06_13525"
     CDS             3952..4866
                     /locus_tag="VFD06_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04598.1"
                     /translation="MTPEPRRPLRPRAARRSFAFVLGSALVGTVAFAALTAPIVGPLF
                     APDPYTQVAPRDRTPPTPPDREHPFGTDAMGRDLVSRVLYGARLSLVVAVGAQAIALA
                     LGLAIGAAAGWLGGAADAFAMRGADLLLALPAPLVALAVAAAVPEPERAPLLRLLPVP
                     SAGLVLLVLAGLGWAGIARLVRGEIVRLRGEAFTGAARAAGAGPLRIVGRHLLPNALG
                     PIVVVATLGLGGNILMEAWLSFLGVGARPPIPSWGTMIAESQIHFLERPWAGLAPGLA
                     ILMAVLGFNLLGDALRDRLDPRSGSMAG"
     gene            4863..6494
                     /locus_tag="VFD06_13530"
     CDS             4863..6494
                     /locus_tag="VFD06_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN04599.1"
                     /translation="MTTAARRFARIVLLGALAGLPLLAAGCGAREGDAPAARQVFRFR
                     LREEPPTLDPALMTDNLSESIVTNVNRGLVAMDPATLAVVPAVASSWTVSPDGLTYTF
                     RLRDNVVFHNGRAVAAEDVLWSFQRLLRKETHSPRRWLLEPIRGAADFAEGRAAAVAG
                     LASPDPRTVAVTLDRPFAPFLAMLTMAGASIVPREVYEDPEKTYLRAPVGCGPFRVVR
                     VEPASLIEMAAFDRYYDGRPAIDIVQARIIENQVTALQEYRTGGLDSLDEVPVDLPDE
                     VRPEVLRYPLMGVGYIGFNLDRPPFKGNAALRQALNYAVDKEYLWGKLIPGANIPGRG
                     IIPPGVPGYDPDLPGYPHDEAKARALLARAGYPAGQGLEPISLWFNTSGDNRRIAEQV
                     QADLRKVGVTLTLREVDWGAYLKAVEGTAQVPGEAQMFRFGWGLDYPDADAILRPLLH
                     SKNIGPAGNYFRYRNPAFDDLVDRALALGDASERAALYRQAERMAVMQDAPWLFLNYF
                     GSMTLFKPYVKGVTPTPLGEFRIPLERLRIEKPAS"
     gene            6514..7491
                     /locus_tag="VFD06_13535"
     CDS             6514..7491
                     /locus_tag="VFD06_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04600.1"
                     /translation="MTGYLLRRLLGLVPVLLAAATLVWGLLFVLPGDPARLIAGARAV
                     DPEVVQEVRAEWGLDDPALVQYVRYLGRLLRGDLGVSYAQGRPVSDILGEHLTPTLAL
                     AVAAVAIAIVAGIGLGTLAAAFRGRGFDLLVLLLALVGASTPVFWLGLLLMLTFASWL
                     RWFPATGYGLDGMVLPLFGGRLPEWRHLALPALTLALVLMGSLARVTRAALLDVAGTE
                     SLRAARARGLSRAGVLLRHALRRALPPVVTVLGLQLAGLLGGAVATEFVFAWPGLGKA
                     LVRAIALRDLPLVEGCVLLLTAAYVLAALAVDLLLPILDPRIGDAAGLQ"
     gene            7512..8576
                     /gene="rlmN"
                     /locus_tag="VFD06_13540"
     CDS             7512..8576
                     /gene="rlmN"
                     /locus_tag="VFD06_13540"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZN04601.1"
                     /translation="MNPSPDIQDIRLNLFGLDREGLREALTPLTARRFHADQVFHWIY
                     GRRETDPRRMSDLPAALRAAIDDRFRFAWPRTRVSGRSADGSRKHLLTLEDGLEIEAV
                     HMVHADRVTFCLSSQVGCALDCAFCLTGTMGLRRQLGAGEIAGQVAALLADGEVPIDQ
                     LRIVFMGMGEPLHNYDAVMAAFRILVDPQGFALPPRRITLSTAGLVPGIERLGREDPR
                     PRLAVSLAAPTDAARARLMPINRKWTLAKLLAACRAFPLGPRERITFEYVLLAGVNDS
                     AGDAEATARLLAGLRAKVNVIPYNEAGLHGFATPAAAVAARFRDVLLRRGMQASIRWS
                     KGRDIGAACGQLARTAASAS"
     gene            complement(8581..9213)
                     /gene="thiE"
                     /locus_tag="VFD06_13545"
     CDS             complement(8581..9213)
                     /gene="thiE"
                     /locus_tag="VFD06_13545"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZN04602.1"
                     /translation="MPGAPALQLVTDRHAVAGRTLADTVEAALRGGVTRVQVRERDLE
                     GAALLRLVEEMMALAARQGKAKISVVVNDRLDVALAAGAAGVHLPAAGLPVKQARAHA
                     GARFRIGRSVHSLAEARAAEKDGASEILFGPVFATPTKAAFGPPQGIEALRKVAEAVR
                     IPVWAIGGINPETAPALRGLPIAGIAAIGAFFASADPAQAAHDLRAALEG"
     gene            complement(9217..9456)
                     /locus_tag="VFD06_13550"
     CDS             complement(9217..9456)
                     /locus_tag="VFD06_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04603.1"
                     /translation="MRRPWLHLLYLLYCAEAGVYLLLVPWSVLWWPMLVGWPSRPRAV
                     LATGVARGAVSALGGLLIVVCIIDLVRFCRAFRSA"
     gene            9569..10396
                     /locus_tag="VFD06_13555"
     CDS             9569..10396
                     /locus_tag="VFD06_13555"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HZN04604.1"
                     /translation="MRIATVPYLNAAPLVWGLGARGAASGFRVEIAPLRPSRIAPALL
                     SGSADVGLVPVADIPGLGTPELVGGLGIASRRRARSVLLVSRRPIAEARRIALDSSSR
                     TSAALVRLLLRARGVDGAEFVERAPEWPAMLDGVDAALVIGDPALTADLTGCEVIDLA
                     SEWHASTGLPFVFAVWAARPGFVLPDGGRMFHEAYRDGAERIDDLARDAAVALGRPAD
                     DLADYLSTCIHYRLGAEEERAIPLFLARAHEIGLVPRPAPLRWRREAVTAEVAGRTA"
BASE COUNT         1269 a         3924 c         3791 g         1543 t
ORIGIN      
        1 ggacggcgcg ctcgacctgc gcagcatctc gggggggctg ctcgcgcagg acggcgacac
       61 gggacgggcc gacgttccgg cagcccgcgt cgtgacccgc cgcgctccga ccgacgaaga
      121 gcgcacagcg ctcgcgttcg catggcgggt cgccaagcac gtcaagtcga acgccatcgt
      181 cctggcccgc gacgagcaca cggtcggggt cggggcaggt cagatgagtc gcgtcgactc
      241 ggttcgcctg gcaatcgcca aggcccagga gccggtgcgt gggacctcgc tcgcgtccga
      301 cgccttcttc cctttccgcg acggggtgga cgaggcggcg aaggccggcg tgaccgcggt
      361 ggtgcagccg gggggctcgg tccgcgacga ggagatcgtc gccgccgcgg acgagcacgg
      421 gatggcgatg ctcgtcaccg ggatgcggca cttccggcac tgatcggggt catcccggcg
      481 acgccggcgc tgtgggccgg cgttacttcc ggtcgaacga tcggacccgc agcgtgtcca
      541 cgtacgagta gaagcctttg tccttcgggc gcgacgcgta ctgcgtcgcc accgccatca
      601 tcttctcggc ctcggcgtcc gccgggtcca cctggaacac ctcgttgagc ttctgggtgg
      661 cggtcgtggc gtcccccccc ttgagcgcca cgaccgccca gttgtaccag gcgttccgga
      721 tcggcacatc gagatcgcca taacgaggat ccttcggcag gcgatacagc ttccacagcg
      781 ccccctggta atccttgtcc tggaacaggc gccgcgcctc gtcgtagtcc tgccggacct
      841 tggtctcctg atcgaccttc gcctgcgcct tcccgagcag ttccttgcac tcgaagttga
      901 tcggatcgag cgccagcgcc gccgccagtt cgcggcgcgc ctcctgccag cgctcctcct
      961 cgaggaacgc cttccccgcg gcgaggtgct gcgcggtctc ctggcggatc tcctcggcgg
     1021 tgcggggccg cgcgggggcg ggcgccggcg cgaccgcgga cttcgccgtt cccggcgcgg
     1081 cgccggacgg ggcgaccgcg accggcgcgg tctcttccac ggatggggcg ggggacggtc
     1141 cgtcggagcc ggtcccctcc tgatgcccga tcggctcggt ctgcttcgac gtcgatccga
     1201 tcggcgtcgg gatcggtccg ggatcggccg cgtcggcggc cgacgaggcg gaagggggcg
     1261 cggcggcggt cggcgcctgt gaccggccgt cggcgtttcg cggctggaag aagaaccacc
     1321 agacgaccgc ggcgaccgcc aggatcacga cgccgcagat ggcgaacagg gaggtcgtgg
     1381 cgctcgcgcg gcgctcgccg gcggggctca tgctggccag gcgcgcggcg ctccgttccc
     1441 gctgacgcgg ccccggacgc acggtcggga tcgcgaacgc cagatcgggc acgggaggcg
     1501 gtgccggggc cggcggcggg atctcggccg gcggcccctc ctgcgaatcg tgagcgggcg
     1561 cctgcagggg gccgatctcg tcccccatga gcgttgtcag gtccaggtct tcgtccgccg
     1621 gggcctcggg cgcggatgcc cgggcggcgg atcgcccgac gatctcgtgc gcgccgctct
     1681 tctggttcag gctctcgagc tcctgctccc agcccgcgtc cggccgcgcc tgcgcggagg
     1741 ccggggccgc cgcgcgggcc ggcgcccccg ggtcggtcgc ggtgacgggc ggggccccgg
     1801 gaacggcgag cgggatcgat tcgatcgctt ccggctcctc gtccagctgc tgcatcagtc
     1861 gatcgagcgc ggcctgcccc tcggcgctcc gctcgacgag ccgcaccgaa ggagggacca
     1921 ccgtggtggc ctcgatcacc tccggctcgc ccggcgcctc gacggccgcc ggcgcggtct
     1981 cctgctcagg gccagcggcc tccgtctccg ggcccgcggc gtccgcctcc ggagcggcga
     2041 cctccagcgg cgcatcgagc gacaatcctt cgatctcgat ctccggcacc tccacgtcgg
     2101 ggatcgcgga cgcgtccgcg cccagatcga gcgagtcccc ggcgaggaaa tccagttccg
     2161 gctccccggc gcctgccgtc tcccccgcct cctcctgagg cggcgcgccg gtgtcggcgg
     2221 acgggtcggc gatttcgatc cggtcggtct ccggcggctc cgtgccgaac gacggctcca
     2281 cgtcgaacgg cgtctcgtcc gccccctccg cctcgcgcga ctcttccgcg gtctcgcgcg
     2341 gtgcctccgc ggccgccgcg gcggccggcg gccccgccgc ggcagggggc gaaggcttcg
     2401 agacctggaa cgcctgcagc tgggagcgat cgatgcgcat cgtccggcca ccgagatcgc
     2461 cggccggctc gggagctgcg ggggcgggcg acgcggcgcc cttgccctgg atcttcacga
     2521 ggaacgcgtg cgcatgaggg ttggccggat cgagcgcgaa caccttgcgg cattccgcct
     2581 ccgcctcgtc gcggcgtccc tggtcgagga gctcctgcga aagcgtcagg tgctcgtcga
     2641 tgaacggggc cgactcgaac gcctgctgcg cctcctcgac caggcgctgc acttcctcgg
     2701 agtccggatc gatcggcacg agcccgcgcg ccccttcgat cgcctcggag tacttgcgct
     2761 cgaggagaag gctcttgacc cgctttactc ccgcatcgat gcgttgggcg tccgcttcgg
     2821 ggctcgccga cggcccggcc ccggcgccgt cggccggccg tgccggctcg gtctcggccg
     2881 gcgcgacggt gggatcccag tcggcgagca gaagcgtcgc catctggatc ccttcacgcg
     2941 ccttctggct cgagggatcg accgccagcg cctcacgcca agccgcgatg gcgccgcgat
     3001 agtcgccatt gttgtagagg ccggtggccc ggacgagcaa ttcctgcaag agttcgtcgt
     3061 cgatcaccag agccccgcgg gcacagatcg gcccggaaaa aaccgtgtcc aaatgtaggt
     3121 cgtgggaccc ggaagtcaaa cgggcgccgc cccgcgggac ggggcgggat caggcgggat
     3181 ttcgggcgcg gcggacctct tccttgagct ccgcgagccc cgggtactcc ggatgcagct
     3241 cctccacctt gccgaacgcc gcggcggcgg tttcgagatc gtgcttgtcg aaaagactca
     3301 cgattccgag gttcagccag gtctgccagt gccgcgggtc ggtgtcgagg gagcggcgga
     3361 actcggcgat ggcgtcgtcc ggccggccca tctcgcgcag acagatgccg agatcggtgc
     3421 gggcgtccac gagcttcccg tcctccgcga gcgcacgacg gtagtactcg atcgcctggt
     3481 cgtacttgcc ggcgtcctgg tagaagttgg ccagccgcac gagcgcctgc acgtcccggg
     3541 ggtttttctc gagcgcttcc ctcagggagg cgatctcctg gaagacgcgg gccatgagct
     3601 cttcgcgacc ggccccgccc tcctgcccct gcgcctccgt cccggcctcg ccgcccccgc
     3661 tgttgcccga ccctgccccg ggcccgatga cggcgccggt catgccgagg ttccccgcct
     3721 cgggaaccgg cgcggcctcg tgcttgacgc gcggcacggc gacaccccag gcgacgacgt
     3781 agccgacgag gaatccgaac aggattcccg acacaaggaa ccgccactgc tcgcgcgtca
     3841 tgcccggacc accctccgca gggaggggac cgtagcacac gggttccggc ggatcaagga
     3901 aggacggacg gcgctcttgc aatcggcggg cggcgtccct atcgtacgcg cgtgacgccg
     3961 gagccccgcc gccccctccg cccgcgcgcc gcgcgccgtt ccttcgcatt cgtgctcgga
     4021 tcggccctcg tgggaacggt ggccttcgcg gccctgaccg ccccgatcgt cgggccgctg
     4081 ttcgcgcccg atccctacac gcaggtggcg ccgcgcgatc ggacgccccc cactcccccc
     4141 gaccgggagc accccttcgg caccgacgcg atgggccgcg atctcgtgtc gcgcgtgctc
     4201 tacggcgcgc ggctgtcgct cgtggtcgcg gtcggtgcgc aggcgatcgc gctcgccctg
     4261 ggtctcgcga tcggcgcggc ggccgggtgg ctcggcggcg ccgccgacgc attcgcgatg
     4321 cggggggccg atctgctcct ggccctgccg gcgccgctcg tggcgctggc ggtggccgcc
     4381 gccgttccgg agccggagcg cgcgccgctt ctgcgcctgc tgccggttcc gtcggccggc
     4441 ctggtgctgc tcgtgctcgc cggactgggc tgggcgggga tcgcgcggct ggtgcggggg
     4501 gagatcgtgc ggctgcgcgg cgaggcgttc accggcgccg cccgcgccgc cggcgccggt
     4561 ccgctgcgga tcgtcggccg gcacctcctg ccgaacgccc tcggaccgat cgtcgtcgtg
     4621 gcgacgctcg ggctcggcgg aaacatcctg atggaggcgt ggctgtcgtt tctcggcgtc
     4681 ggagcgcgcc cgccgatccc gtcgtgggga acgatgatcg ccgagtcgca gatccacttc
     4741 ctcgagcgtc cgtgggcggg gctcgccccg ggactggcca tcctcatggc ggtgctcggc
     4801 ttcaacctgc tcggcgacgc cctgcgcgat cgcctcgatc cgaggtccgg gagcatggcg
     4861 ggatgacgac cgcggcccgc cgcttcgccc ggatcgtcct gctcggcgct ctcgccgggt
     4921 tgccgcttct ggccgccggc tgcggcgcgc gcgaaggcga cgcgccggcg gcgcgccagg
     4981 tcttccgctt ccggctgcgc gaggagccgc cgaccctcga cccggccctg atgaccgaca
     5041 acctgtcgga gtcgatcgtc acgaacgtga accgcggcct cgtcgcgatg gacccggcga
     5101 cgctcgcggt cgtgccggcg gtcgcctcct cctggaccgt gtcgcccgac ggcctgacgt
     5161 acacgttccg cctgcgcgac aacgtcgtgt tccacaacgg acgcgccgtc gcggcggagg
     5221 acgtcctctg gtccttccag cgcctgctcc ggaaggaaac gcattcgccg cgccgctggc
     5281 tgctggagcc gatccgcgga gccgcggact tcgccgaggg aagggcggcg gcggtcgccg
     5341 gcctcgcctc ccccgacccg cgcacggtgg cggtgacgct ggaccggccg ttcgccccgt
     5401 tcctcgcgat gctgacgatg gccggggcgt cgatcgtgcc gcgcgaggtg tacgaggatc
     5461 cggagaagac gtacctgcgc gccccggtcg gctgcggtcc gttccgggtg gtgcgcgtcg
     5521 agccggcgag cctcatcgag atggcggcgt tcgatcgtta ctacgacggc cgcccggcga
     5581 tcgacatcgt gcaggcgcgc atcatcgaga accaggtgac cgcgttgcag gagtaccgca
     5641 ccggggggct cgactccctc gacgaggtgc cggtcgatct ccccgacgag gtgcggccgg
     5701 aggtgctgcg ctacccgctg atgggggtcg gctacatcgg cttcaacctc gaccggccgc
     5761 cgttcaaggg gaacgcggcg ctgcgccagg cgctcaacta cgcggtcgac aaggagtacc
     5821 tgtgggggaa gctgattccc ggagccaaca tcccggggcg cggcatcatc ccgcccggcg
     5881 tcccaggcta cgacccggac ctccccggct acccgcacga cgaggcgaag gcccgggcgc
     5941 tcctggcgcg cgccggctac ccggccggtc aggggctgga gccgatctcc ctgtggttca
     6001 acaccagcgg cgacaatcga cgcatcgccg agcaggtcca ggccgatctg cgcaaggtcg
     6061 gcgtcaccct gacgctccgg gaggtggact ggggcgccta cctgaaggcg gtggagggga
     6121 ccgcgcaggt gcccggcgag gcgcagatgt tccgcttcgg atgggggctc gactaccccg
     6181 atgccgacgc gatccttcgc ccgctgctcc actcgaagaa catcggtccg gccggcaact
     6241 acttccgtta ccgcaacccc gccttcgacg atctcgtcga ccgcgcgctc gccctggggg
     6301 acgcgtcgga gcgggcggcg ctgtaccgcc aggcggagcg gatggcggtg atgcaggacg
     6361 cgccgtggct cttcctgaac tacttcggct ccatgacgct cttcaagccg tacgtgaagg
     6421 gggtcacgcc gacccctctc ggcgagttcc ggatccctct ggagcgcctg cgcatcgaga
     6481 agccggcatc ctgatcgcgg tcgggcgcgg cgcgtgaccg gctatctcct gcggcgcctc
     6541 ctggggctcg tgccggtgct gctcgcggcc gcgacgctcg tctgggggct cctgtttgtc
     6601 ctccccgggg acccggcccg cctcatcgcc ggggcccgcg ccgtcgatcc ggaggtggtg
     6661 caggaggtgc gcgccgagtg gggcctggac gatccggcgc tcgtccagta cgtccggtat
     6721 ctcggtcgcc tgctgcgcgg cgacctcggc gtgtcgtacg cgcaggggcg gccggtgagc
     6781 gacatcctcg gcgagcacct gaccccgact ctggcgctgg ccgtcgccgc ggtggcgatc
     6841 gcgatcgtcg ccgggatcgg cctcgggacg ctcgccgccg cgttccgcgg acggggcttc
     6901 gacctcctcg tcctgctcct cgcgctcgtc ggcgcctcga cgccggtctt ctggctcggc
     6961 ctcctcctca tgctgacctt cgcgtcgtgg ctccgctggt tcccggcgac cggctacgga
     7021 ctcgacggga tggtcctgcc gctgttcggc gggcgcctcc cggagtggcg gcacctggcg
     7081 cttccggccc tgaccctggc gctcgtcctc atggggtcgc tggcgcgggt gacgcgcgcg
     7141 gcgctcctcg acgtcgccgg gacggagtcg ctgcgcgctg cgcgggcgcg cggcctcagc
     7201 cgggcgggcg tgctcctgcg ccatgcgctg cgccgggccc tgccgccggt ggtgacggtc
     7261 ctcggcctgc agctcgcggg gctgctcggc ggggcggtcg cgaccgagtt cgtgttcgcc
     7321 tggccggggc tcggcaaggc cctggtgcgg gcgatcgcgc tgcgggatct gccgctggtg
     7381 gaagggtgcg tgctgctgct cacggcggcc tacgtcctcg ccgccctggc ggtcgatctg
     7441 ctgctcccga tcctcgaccc gcggatcggg gacgccgcgg ggctacaatg accgctccgg
     7501 agcccacccc catgaacccc agccccgaca tccaggacat acgtctgaac ctgttcggcc
     7561 tcgaccgcga ggggctgcgg gaggcgctca ccccgctcac ggcgcgccgc ttccacgccg
     7621 atcaggtgtt ccactggatc tacgggcgcc gcgagaccga tccgcggcgg atgagcgacc
     7681 tgccggcggc cctgcgcgcg gccatcgacg atcgcttccg cttcgcctgg ccgcgcaccc
     7741 gcgtcagcgg gcggtcggcc gacggctccc gcaagcacct cctcacgctc gaggacggcc
     7801 tggagatcga ggcggtgcac atggtgcacg ccgatcgcgt caccttctgc ctgtcgtcac
     7861 aggtcggctg cgccctcgac tgcgccttct gcctcaccgg aacgatggga ctgcgccggc
     7921 agctcggcgc gggcgagatc gccgggcagg tggccgccct cctcgcggac ggggaggtgc
     7981 cgatcgatca gctgcggatc gtcttcatgg ggatgggcga gccgctgcac aactacgacg
     8041 ccgtcatggc cgcgttccgg atcctcgtcg acccacaggg attcgcgctg ccgccgcggc
     8101 gcatcaccct gtcgacggcc gggctcgtcc ccgggatcga gcggctcggc cgcgaggacc
     8161 cgcgcccgcg cctggcggtg tcgctcgccg cgccgaccga cgcagcgcgc gcgcggctca
     8221 tgccgatcaa ccggaaatgg acgctcgcca agctgctcgc cgcgtgccgg gcgttcccgc
     8281 tcgggccgcg cgagcggatc accttcgaat acgtcctcct cgccggcgtc aacgactccg
     8341 cgggggacgc cgaggcgacg gcgcgactgc tggccggtct gcgcgccaag gtgaacgtga
     8401 tcccctacaa cgaggcgggg ctccacggct tcgcgacgcc ggccgcggcg gtggcggcgc
     8461 ggttccgcga cgtgctgctg cgccgcggca tgcaggcgtc gatccgctgg agcaaggggc
     8521 gggacatcgg cgccgcctgc ggccagctgg cacgaacggc cgcgtcggcg tcctagtttc
     8581 ctaaccttcc agcgcggctc gcagatcgtg cgccgcctgc gccggatcgg cggacgcaaa
     8641 gaaggcgccg atggcggcga tgccggcgat ggggagtccg cgcagggcgg gggccgtctc
     8701 ggggttgatg ccgccgatcg cccagacggg aatccgcacc gcctcggcga ccttccggag
     8761 cgcctcgatc ccctgcggcg ggccgaacgc cgccttggtg ggggtcgcga agaccggccc
     8821 gaacaggatc tcgctcgctc catccttctc ggcggcgcgc gcttcggcga ggctgtgcac
     8881 cgagcggccg atccggaagc gcgccccggc gtgagcgcgc gcctgcttca cgggcagccc
     8941 ggccgccggc aggtgcaccc cggccgctcc ggcggcgagc gcgacgtcga gccgatcatt
     9001 gacgaccacg ctgattttcg cctttccctg ccgggcggcc agcgccatca tctcctcgac
     9061 gagacggaga agcgccgccc cctccagatc ccgctcccgc acctgcaccc gcgtcacgcc
     9121 cccccgcagc gccgcctcca ccgtgtcggc gagcgtccgg ccggccacgg cgtgtcggtc
     9181 ggtcacgagc tgcagggcgg gggcgccggg cacggatcag gcgctccgga aggcgcggca
     9241 gaagcgcacg aggtcgatga tgcagacgac gatcaggagc ccgccgagcg ccgacacggc
     9301 tccacgcgcc accccggtcg cgagcaccgc gcgcgggcgg gacggccagc cgacgagcat
     9361 cggccaccac agcaccgacc agggcacgag caacagataa accccggcct cggcgcagta
     9421 aagaagatac agaagatgga gccacggacg tcgcatcggc accggcaaat gtaacagaga
     9481 taccggcccc gtccaccccg cccgccgcgg cgaacgactc tggtagaatc ccccgccaca
     9541 tcaccgcgcg aggtttcccg tgcagcccat gcgcatcgcg accgttccgt acctgaacgc
     9601 cgcgcccctc gtctgggggc tcggggcgcg cggcgccgcc tccgggttcc gcgtggaaat
     9661 cgccccgctt cgtccgtccc gcatcgcacc ggctctgctg tcgggatcgg ctgacgtcgg
     9721 tctcgttccg gtcgcggaca tccccgggct cggcacaccg gagctcgtcg gcggtctcgg
     9781 catcgcgtcc cggaggcgcg cccgctccgt gctgctggtg tcccgccggc cgatcgcgga
     9841 ggcgcggcgg atcgcgctcg attcctcctc gcggacctcc gccgcgctcg tgcggctgct
     9901 gctgcgggca cgaggggtgg acggcgcgga attcgtcgaa cgggcccccg agtggccggc
     9961 gatgctcgac ggcgtcgacg cggcgctggt catcggcgac ccggctctca ccgcggacct
    10021 gaccggctgc gaggtgatcg atctcgcctc cgagtggcac gcctcgaccg ggctcccctt
    10081 cgtcttcgcg gtctgggcgg cccgtcccgg cttcgtcctt ccggacggcg gccggatgtt
    10141 ccacgaggcg taccgcgacg gggcggagcg gatcgacgac ctcgcccgcg acgccgcggt
    10201 cgcgctggga cggcccgccg acgacctggc ggactacctg tcgacctgca tccactaccg
    10261 cctgggggcg gaggaggagc gggcgatccc cctgttcctc gcgcgggcgc acgaaatcgg
    10321 gctcgtcccg cgcccggcgc cgctgcgctg gcgccgtgag gcggtcaccg ccgaggtcgc
    10381 cgggaggacc gcgtgagcgc cggaacgacg gcgatcccgg cctcggcgcc cgagcggcgg
    10441 cgcgtacgac gatcccggcc ggagaccgag accatcctcg agcgggcgat cgaggggacg
    10501 cggatgaccc cggaggaggc gctgctc
//
LOCUS       DASRIQ010000547         7001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2546993, whole genome shotgun sequence.
ACCESSION   DASRIQ010000547 DASRIQ010000000
VERSION     DASRIQ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2546993"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VFD06_13560"
     CDS             complement(<1..120)
                     /locus_tag="VFD06_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HZN04605.1"
                     /translation="MRYYALATDYDATLATDSRVDEATVQALEELARTGRRLIL"
     gene            224..4972
                     /locus_tag="VFD06_13565"
     CDS             224..4972
                     /locus_tag="VFD06_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04606.1"
                     /translation="MTSVWRLFFVPVSALIVSGAVSGGGPEPAPPGPAAPERGASIRF
                     GPQHTLEIPAVLREGLRRPGTAAAMGGGSEQGEPTMGPRPTALLLGDLDADGVPERIS
                     GWAAGTGGALFVEPGSPGSGSEDTGELVEIAEAPEFVAIGDFDGDADTDLLLAGRGER
                     RLLFLAGDGTGRLDRPRTVMLDGDVTALATGEIDRADGLPDVLVAVDGRGGPRLLLFA
                     SPHGLRDHRAESLPLPEAATDLAFGALDGDRSSDLAIAAGRTLLILHGRDVEAEDDAR
                     APGRAAFEMHRFPDRIVSLAAGDFVLDEAARTDLALLTEGGDLYRLAPSADGRDRFLG
                     AAPHPAAPRRDDGFEARHLTSARVSTRPGDDLILWSDSDRRLLVLAAAARPEVEPAEV
                     AFVDAGRPPVRVAPVPSAASRLNGLAIVEAESGGVTLIEAATTTVFTVNSTLDNPDIT
                     PGNGACNTSVLMACTLRAAIMEANALPGADVIQFAIGAGTPTLAPFSALPVITETITI
                     NGNSGGASRVQIDGASVIATPDGLRLGSTATSSSGGSLLRDLVIRGFAGYGVRIETDG
                     SALERLSVGTDAMGGGTVAGNAAGGILITGAGATANSIGGSLATRCIVSHNGGAGITI
                     EAGAASNKIGGNFIGLSAVGAALANAGSGMKIQSGAHDNVVGQPTASPGMAPGNVLSA
                     NGDAGVEITGTGSFGNLVQGNLIGLAPSGVTGLANAVTGVRIAMAAQSNTIGGTTIAQ
                     GNVISGQTNPGSDGVRITGAGTAGNFVRGNLIGTDITGAAALGNAQDGVHIDGGAMTN
                     IIGGQTPTPGTPPGNVISGNVMNGVRILDAGTTNTVVLGNLIGTNVTGAAHLRNGSTG
                     VTIDGAPGNLVGGSAVTARNIISGNVSDGVLIENPGANNNRIWGNFIGTTVTGGAPLG
                     NLGAGVRIVCGGIPNTPPALTGTAIGAEAGVPGAPPGNVISGNTLFGVVIEGEGADKN
                     TVSGNLIGTDAAGTGALGNLQDGVRVHGGADDNRIGGGSVAARNVISGNASSAASDGV
                     EVTPNTTNSTQIEGNFIGTDITGMAALPNGGCGVFVNDGPSGTLVGNLTPTPGTPPGN
                     VISGNDINPTSHGVLITGMGSGDNQVLGNIIGLNAAGTAPLPNRGDGVQISGNATLDR
                     VGSGVPGASNVISGNNLSLASSGVRIADGNDNIVQGNLIGLLASGMGPAGNGAAGVVI
                     TSATAAGAEGNRIGGTASGERNVISGNGLQGVLINTSTTGHNFVQGNFIGTDVTGAGA
                     QGNGSYGVLVTGGSDFNVIGGTTGATAGDCTGGCNLIAYNLDAGVFIGNGTCTSPGAG
                     CSVNNEIRGNRIHDNAALGIDLMPAGPDLNDIGTLDGDFGANNRQNYPDIISASSIAG
                     TITVGGSLSSSFATSFIIDIYGNTTVDPSNFGEGATWLGSTTCVTTGGSGTCLWNATL
                     PGDAAYLTATATIDSPLPADTSEFSLRFGNPGEVPMLLLAPGAPGSLVATYTPACGAS
                     GHVLYMTTGPLGPGTFWSEQFCAPGASGTLIFTPPPLAIGQVQHFVMVGQNSSVEGSY
                     GTDSSGTERPEAGPFGTFCDRPQALGVSCP"
     gene            5049..5597
                     /locus_tag="VFD06_13570"
     CDS             5049..5597
                     /locus_tag="VFD06_13570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN04607.1"
                     /translation="MPLENQDQADVAIPDQDLILLRRRLHDAATRLCRGPLTLHLEDI
                     VQTALLRVREALLRGDVERSVPASYLSRAAFSALVDEVRRQRRRREVPVEEVRDVPLP
                     RHHPGDPERAGGDADIRRAIRACLATLVPPRRLAVTLHLQGHSGREAARLLAWAEKRV
                     NNLIYRGLGDLRRCLASKGFAP"
     gene            5594..6244
                     /locus_tag="VFD06_13575"
     CDS             5594..6244
                     /locus_tag="VFD06_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04608.1"
                     /translation="MNESTDRDARLRSAFDGPPGPDERRDDCPPADAIWRARSGDLDA
                     TARRVLIDHTAACAECAEAWRVAGDLGAGEGLAAARPDRRAWLRTAGLAAALLLALGL
                     VVWRQSRGRQGTDEYRDGASLAIRTLTPEDRPQPRSGLSLRWTPGPAGTTYDVSLTTE
                     ELLPVARARDLAEPRFDVPAAATGRLPAGATLVWRVVARLPDGSLVTSPAFVVRLE"
     gene            6241..>7001
                     /locus_tag="VFD06_13580"
     CDS             6241..>7001
                     /locus_tag="VFD06_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04609.1"
                     /translation="MRGVALRLAAALLLASIPSSPRAALSPEATLEDCDALVRAHPDE
                     KDSYRCYYEVARRRAVWDDAERRLQTHRASSDYALVMLAVLTSARGRADEAQGLYREA
                     IAAFAARGNRAEEGLARLGLARTLRRAGRAAEAAVELDGAEALGQATGDRALVSWVRL
                     ERASQLEAEGRDYGAAYRLLRQVETDAFPDGPYQLRINCLGALARLTQFLGRNQESLA
                     SLRRVEETARAAGDKANVAVALTNRLYMELDLVGE"
BASE COUNT         1020 a         2581 c         2496 g          904 t
ORIGIN      
        1 cagaatcagg cggcgtcctg tgcgggccag ctcctcgagc gcctggaccg tcgcctcatc
       61 gacccggctg tcggtcgcca gcgtggcgtc atagtcggtg gcgagggcgt aataccgcat
      121 ccgtctactc tacggagaaa gccgcgcggc ggctaccgag gagcaactga ggaaaagccg
      181 ggctcccgtt cgtcacttcc ccgaaggcca agggaggtga cccatgacgt ccgtctggcg
      241 gctcttcttc gttccggtgt cggcgctgat cgtcagcggt gcggtgagcg ggggcggccc
      301 ggagccggct cctcccgggc cggcggcgcc ggagcgcggc gcatccattc gcttcggccc
      361 gcagcacacg ctcgagatcc cggcggtcct ccgggaaggg ctgcgacggc cgggcaccgc
      421 cgcggccatg ggcggcggat cggagcaggg cgagcccacg atggggccgc ggccgacggc
      481 acttctcctc ggcgacctgg acgcggacgg cgtccccgag cggatctccg gctgggccgc
      541 cgggacgggc ggagcgctct tcgtcgagcc cggctctccg gggagcggat ccgaggacac
      601 cggcgaactc gtggagatcg cggaggcccc cgagttcgtc gcgatcggcg atttcgacgg
      661 cgacgcggac acggacctcc tgctggcggg gcgcggcgag cgccggctcc tgttcctggc
      721 cggcgacggg accggccggc tcgaccggcc tcgcacggtg atgctcgacg gcgacgtcac
      781 ggcccttgcg accggagaga tcgaccgcgc cgacggcctt ccggacgtcc tggtcgcggt
      841 cgacggacgg ggcggcccgc ggctgctgct gttcgcctcg ccgcacggac tgcgcgatca
      901 ccgggcggag agcctgcctc tccccgaggc ggcgaccgat ctcgccttcg gcgcgctcga
      961 cggcgatcgc tcttccgacc tggcgatcgc cgccggccga acgctcctga tcttgcacgg
     1021 gcgcgacgtc gaggccgagg acgacgcccg cgcgccggga cgagcggcgt tcgagatgca
     1081 tcgcttcccg gaccgcatcg tttcgctcgc ggcgggcgac ttcgtcctcg acgaggccgc
     1141 gcgcaccgac ctggcgctcc tgaccgaggg gggcgacctg taccgtctgg ccccctcggc
     1201 ggacgggcgg gatcggttcc ttggggccgc gccccaccct gcggcgccac ggcgcgacga
     1261 cgggttcgag gcgaggcacc tgacgtccgc ccgggtctca acccgccctg gagacgacct
     1321 gatcctctgg tcggactccg accggcggct gctcgtcctg gccgccgcgg cgcgccccga
     1381 ggtggagccg gccgaggtgg cgttcgtcga cgcgggccgt ccgccggtgc gtgtcgctcc
     1441 ggtgccgtcg gccgccagcc ggctcaatgg cctggcgatc gtcgaggcgg agtccggcgg
     1501 agtgaccctc atcgaagcgg cgacgaccac cgtcttcacc gtgaactcga cgctcgacaa
     1561 ccccgacatc acgcccggga acggggcatg caacacgtcg gtcctcatgg cctgcacgct
     1621 gcgcgcggcg atcatggagg cgaacgcgct tcccggcgcc gacgtcatcc agttcgcgat
     1681 cggcgccggg acgccgaccc tggcgccgtt ctcggcgctg ccggtcatca cggaaacgat
     1741 cacgatcaac ggcaacagcg gcggcgccag ccgcgtgcag atcgacgggg cgagcgtcat
     1801 cgccacgccg gacggcctgc gcctcggctc gacggcgacc tccagcagcg gcggctcgct
     1861 gctgcgcgac ctcgtcatcc gcggcttcgc cggctacggc gtgcgcatcg agaccgacgg
     1921 cagcgcgctc gaacggctct ccgtcggcac cgacgcgatg ggaggcggga ccgtggccgg
     1981 caacgccgcg ggcgggatcc tgatcaccgg cgccggggcg acggccaaca gcatcggcgg
     2041 ctcgctggcg acgcgctgca tcgtctccca caacgggggg gccggcatca cgatcgaagc
     2101 cggcgcggcg tccaacaaga tcggcggcaa cttcatcgga ctctccgccg tcggcgcggc
     2161 gctcgccaac gccggctcgg gcatgaagat ccagtccggc gcgcacgaca acgtcgtcgg
     2221 gcagccgacc gcgtctccgg gcatggcgcc ggggaacgtc ctctccgcca acggcgacgc
     2281 gggggtggag atcaccggca ccggatcgtt cggaaacctg gtgcagggga acctcatcgg
     2341 tctcgcgccg tcgggcgtga ccggcctcgc gaacgccgtc accggcgtcc ggatcgccat
     2401 ggcggcgcag tccaacacca tcggcggcac gacgatcgcc caggggaacg tcatctcggg
     2461 acagaccaac cccgggagcg acggggtacg gatcaccggc gccggcacgg cgggcaactt
     2521 cgtccgcggc aatctgatcg gcaccgatat cacgggagcg gcggccctcg gaaacgccca
     2581 ggacggcgtc cacatcgatg gcggcgccat gaccaacatc atcggcggcc agacgccgac
     2641 gccggggacc cccccgggga acgtgatctc ggggaacgtc atgaacggcg tgcggatcct
     2701 ggacgcggga acgaccaaca cggtcgtgct cggcaacctg atcggcacca acgtcacggg
     2761 cgccgctcac ctccgcaacg gctcgaccgg cgtcaccatc gacggcgctc ccggcaacct
     2821 cgtcggcggc tccgcggtca cggcgcgcaa catcatctcg gggaacgtct ccgacggcgt
     2881 cctgatcgag aacccggggg cgaacaacaa ccggatctgg gggaatttca tcggcaccac
     2941 cgtgaccggc ggcgcgccgc tcgggaacct cggcgccggg gtgcggatcg tctgcggcgg
     3001 aatccccaac accccgcccg cgctgaccgg cacggcgatc ggcgccgagg ccggcgtccc
     3061 cggagcgccc ccgggcaacg tcatttcggg caacaccctg ttcggcgtcg tgatcgaggg
     3121 ggagggggcc gacaagaaca cggtgtcggg gaacctgatc ggcaccgacg ccgccggcac
     3181 gggcgccctc ggcaatctcc aggatggcgt gcgggtccac ggcggggccg acgacaaccg
     3241 gatcggcggc gggagcgtcg cggcgcggaa cgtgatctcc ggaaacgctt cgtccgcggc
     3301 gagcgacggc gtcgaggtca cgcccaacac caccaactcc acgcagatcg aggggaactt
     3361 catcggcacc gacatcaccg gcatggcggc gctgccgaac ggaggctgcg gcgtcttcgt
     3421 caacgacggg ccgtccggca cgctcgtcgg caacctcacg ccgacgccgg gaacgccgcc
     3481 ggggaacgtg atctcgggga acgacatcaa tccgacgtcg cacggcgtgc tgatcaccgg
     3541 catgggaagc ggcgacaacc aggtcctcgg caacatcatc ggattgaacg ccgcaggcac
     3601 cgcccccctg ccgaatcgcg gcgacggcgt ccagatctcg gggaacgcga cgctcgaccg
     3661 ggtcggctcc ggcgtgccgg gcgcaagcaa cgtgatctcc ggcaacaacc tgtcgctcgc
     3721 cagcagcggc gtgcggatcg ccgacggcaa cgacaacatc gtgcagggga acctgatcgg
     3781 gctgctcgct tcgggaatgg ggcccgccgg gaacggggcg gccggggtcg tcatcacctc
     3841 ggcgacggcg gccggcgccg agggcaatcg gatcggcggc accgcttccg gggaacgcaa
     3901 cgtcatctcc ggcaacgggc tgcagggggt gctcatcaac acctccacga ccggacacaa
     3961 cttcgttcag ggaaacttca tcggcaccga cgtcaccggc gccggcgcgc aggggaacgg
     4021 ctcgtacggc gtcctggtga ccggcggatc cgatttcaac gtcatcggcg gcacgaccgg
     4081 agccacagcc ggcgactgca ccggcggctg caacctcatc gcctacaacc tcgacgccgg
     4141 cgtcttcatc ggcaacggta cctgcacctc tcccggcgcc ggctgcagcg tgaacaacga
     4201 gatccgcggc aaccggatcc acgacaacgc cgccctcggc atcgacctga tgccggccgg
     4261 accggacctc aacgacatcg ggacgctcga cggcgatttc ggggcgaaca accggcagaa
     4321 ctatccggac atcatttcgg cgtcgtcaat cgcagggacc atcacggtcg gggggagcct
     4381 gagcagctcg ttcgcgacct ccttcatcat cgacatctac ggcaacacga cggtcgaccc
     4441 gtccaatttc ggcgaaggag cgacctggct cgggagcacg acctgcgtca ccaccggggg
     4501 gtcgggcacc tgcctgtgga acgcgaccct cccgggcgac gccgcgtacc tgaccgcgac
     4561 ggcgacgatc gactccccgc tgccggccga cacctcggag ttctccttgc ggttcggcaa
     4621 cccgggggag gtgccgatgc tgctcctcgc gccgggcgcc cccggctcgc tcgtcgccac
     4681 ctacacgccg gcgtgcggcg ccagcggcca cgtcctctat atgaccaccg gaccgctcgg
     4741 gcccgggacc ttctggagcg agcagttctg cgcgccggga gcgtccggca cgctcatctt
     4801 cactcctccg cccctggcga tcggccaggt gcagcacttc gtcatggtcg gacagaacag
     4861 ctcggtcgaa ggctcgtacg gcaccgactc gtcggggacc gagcgacccg aggccggccc
     4921 gttcggaacc ttttgcgacc ggccgcaggc gctcggcgtc tcgtgcccgt gagacggtcc
     4981 gctcgccggg tgcactaaga tgagcggcac cgggcgccgg gagaccggcg cccccccggt
     5041 cgatccggat gccgctcgag aaccaggacc aggccgacgt cgcgatcccg gatcaggatc
     5101 tgatcctgct gcggcgccgc ctgcacgacg ccgcgacccg gctctgccgc ggcccgctga
     5161 cgctgcacct cgaggacatc gtccagaccg cgctgctgcg ggtgcgcgag gcgctgctgc
     5221 gcggtgacgt ggaacggagc gtcccggcgt cgtacctgtc gcgcgccgct ttctcggcgc
     5281 tcgtcgacga ggtccgccgc caacggcggc ggcgggaggt cccggtggag gaggtgcgcg
     5341 acgtgccgct gccgcgacac catccgggcg atccggagcg ggccggcggc gacgccgaca
     5401 tccggcgcgc tatccgcgcc tgcctcgcga cgctcgtccc gccccgccgc ctcgccgtca
     5461 cgctgcacct gcaggggcat tcggggcgcg aggcggcgcg cctcctggcc tgggccgaga
     5521 agcgggtcaa caacctgatc taccggggtc tcggcgacct gcgccgctgc ctggcgtcga
     5581 aggggttcgc gccatgaacg agagtacgga cagggacgcg cggctgcgat cggcgttcga
     5641 cgggcccccc ggaccggacg agcggcggga cgactgtccg ccagccgacg ccatctggcg
     5701 cgcgcgcagc ggcgacctcg acgcgacggc acggcgcgtc ctgatcgatc acaccgccgc
     5761 ctgcgcagaa tgcgccgagg cctggcgcgt ggcaggcgac ctcggcgccg gcgaagggct
     5821 ggccgcggcg cgcccggacc ggcgcgcctg gctccggacg gccggtctcg cggcggccct
     5881 cctcctggct ctcggcctgg tcgtctggag gcagtccagg ggtcgccagg gaacggacga
     5941 gtaccgcgac ggcgcctcgc tggcgatccg cacgctgacg cccgaagacc gcccgcagcc
     6001 gcgctccggc ctgtcgctgc gctggacgcc gggccccgcg gggacgacct acgacgtctc
     6061 gttgaccacc gaggagctcc tcccggtggc ccgggcccgc gatctcgcgg agccgcgctt
     6121 cgacgtcccc gcagcggcca ccgggcgtct gcccgccggc gcgaccctcg tctggagggt
     6181 cgtggcgcgc ctgccggatg gcagcctcgt gacgtcgccc gccttcgtcg tgcggctcga
     6241 atgagaggcg ttgccctccg gctcgcagcc gcgcttctgc tggcatcgat cccgtcgtcg
     6301 ccgcgcgccg cgctctcccc cgaggcgacg ctcgaggatt gcgacgcgct cgtccgtgcc
     6361 cacccggatg agaaggactc gtatcgctgc tattacgagg tcgcgcgccg ccgcgccgtc
     6421 tgggacgacg ccgagcggcg cctccagacg caccgtgcgt cgagcgacta cgccctcgtg
     6481 atgctggcgg tcctgacctc ggcgcgcggc cgggccgacg aagcgcaggg gctctaccgt
     6541 gaagcgatcg cggccttcgc ggcgcgaggg aatcgcgcgg aggaggggct ggcccgactt
     6601 ggcctggcgc gcacgctgcg ccgcgccggc cgggccgcgg aagcggccgt cgagctcgac
     6661 ggcgctgagg ctctgggaca ggcgaccggc gaccgcgccc tcgtttcctg ggtccggctg
     6721 gagcgcgcca gccagctcga ggcggaaggg cgcgactacg gggcagcgta ccggttgctg
     6781 cggcaggtgg agacggacgc gttccccgat ggcccgtacc agctccggat caactgcctc
     6841 ggcgcgctgg cgcgcctgac ccagttcctc ggccgcaacc aggaatccct cgcctccctg
     6901 cgtcgggtcg aggagaccgc gcgggccgcg ggagacaagg ccaacgtggc ggtcgcgctc
     6961 accaaccgcc tctacatgga gctggacctc gttggggagg a
//
LOCUS       DASRIQ010000548         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2548219, whole genome shotgun sequence.
ACCESSION   DASRIQ010000548 DASRIQ010000000
VERSION     DASRIQ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2548219"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFD06_13585"
     CDS             <1..318
                     /locus_tag="VFD06_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZN04610.1"
                     /translation="SAVAEVECLRTLDRLRHRGDLTDRDVSARREAVYRLLDEVTVVE
                     PTRPILGRAGEPLPSPLGTLDAIHLATALAWRDTTGADLVFATHDAALATAARSMGFQ
                     TIG"
     gene            complement(371..>2134)
                     /locus_tag="VFD06_13590"
     CDS             complement(371..>2134)
                     /locus_tag="VFD06_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HZN04611.1"
                     /translation="ADVRFTSPYGPTIRKWLKQGIGVHHAGLLPRYRVLIERLAQGGL
                     LKVICGTDTLGVGINVPIRTVLFTKLCKYDGQKTAVLSARDFHQIAGRAGRKGFDDEG
                     FVVAQAPEHVVENLRLAEKAKDGKKFVRRKPPEHNFANWDHATFTRLIGAPPERLASR
                     FEVSHGMLLNVLSRRGDGCAAMRALIRDSHETDRAKTAHFRRGWQLFRALVKRGIVEI
                     VPRSAEGAHAGAPEGSRVRVNVELQEDFSMNQALSLYLLETLPLLDAAVETYPFDLLT
                     LVESILENPELILRRQIDKLKDRAVAEMKAAGLDYDQRMEKLEAIEHPKPLREFIYST
                     FNAFADRHPWVGEENIRPKSIAREMYEGFRSFADYVQEYDLERAEGLLVRHLTSVYKT
                     LVQTVPDRTKTDAILEIEFYLRDMLRRVDSSLLEEWQRMLDPARGGAAGAGGAGPPAR
                     PEEPPDITRDAKTFTASIRARLFAFLRDWSIERDESALEGIDVLDDMDGRPWTPERLR
                     EAREAYRLDHTPARLDPEARNLRHTRVEPSEDGSRWTVQQTLVDPDGHNDWILEVEAD
                     LAASRAAGEPVLRLRRIGSLV"
BASE COUNT          296 a          755 c          732 g          351 t
ORIGIN      
        1 agcgccgtgg ccgaggtcga gtgtctccgg acactcgacc gcctgcggca ccgcggcgac
       61 ctgaccgacc gcgacgtgtc ggcgcgccga gaagcggtct atcggctgct cgacgaggtg
      121 acggtcgtcg agccgacgcg ccccatcctg ggccgcgccg gagagccact cccgtccccc
      181 ctcgggacgc tcgacgccat ccatctcgcc accgccctcg cctggcggga cacaaccggc
      241 gccgacctgg tcttcgccac gcacgacgcc gcgctcgcca ccgccgcccg ctccatgggg
      301 ttccagacga tcggttgagc gtccgcgatc cgtgccaaga ccgagccgga tgttttccac
      361 tcgagcggtc ttagaccagg ctcccgatcc gccgcagcct cagcaccggc tcgccggcgg
      421 cgcgtgaggc ggcgaggtcg gcctcgacct ccaggatcca gtcgttgtgg ccgtcaggat
      481 cgacgagggt ctgctgcacg gtccagcggg agccgtcctc ggacggctcc acccgcgtgt
      541 gacgcaggtt gcgcgcctcg gggtcgagcc gcgcgggggt gtgatcgagc cggtaggcct
      601 cccgcgcctc gcgcagacgc tccggcgtcc acggccgccc gtccatgtcg tccagcacat
      661 cgatcccttc gagcgccgat tcgtcccgct cgatcgacca gtcgcgcagg aacgcgaaga
      721 gcctggcgcg gatcgaggcg gtgaaggttt tcgcgtcgcg cgtgatgtcg ggtggctcct
      781 ccgggcgcgc cggcggcccg gcgccgccgg cgcccgccgc ccctccccgc gccgggtcga
      841 gcatccgctg ccactcctcg agcaggctcg aatcgacgcg gcgcagcatg tcgcgcaggt
      901 agaactcgat ctcgaggatg gcgtcggtct tggtccggtc ggggacggtc tgcaccagcg
      961 tcttgtagac gctggtgagg tggcgcacga gcagcccctc ggcccgctcc aggtcgtact
     1021 cctggacgta gtcggcgaag gatcggaacc cctcgtacat ctcgcgcgcg atcgacttgg
     1081 ggcggatgtt ctcctcgccg acccaggggt ggcggtcggc gaaggcgttg aaggtcgagt
     1141 agatgaactc gcgcagcggt ttcgggtgct cgatcgcctc cagcttctcc atgcgctggt
     1201 cgtagtcgag cccggcggct ttcatttcgg cgaccgcccg gtctttgagc ttgtcgatct
     1261 gccttctcag gatcagctcg gggttctcga ggatgctctc gaccagcgtc aggagatcaa
     1321 aaggataggt ctccacggcg gcgtcgagca gggggagggt ctccaggagg tagagcgaca
     1381 gcgcctggtt catcgagaag tcttcctgga gctcgacgtt gacgcgcacc cgcgagcctt
     1441 ccggcgcacc cgcgtgcgcc ccctcagcgg agcgcgggac gatctccacg atgccgcgct
     1501 tcacgagggc ccggaacagt tgccagccac gccggaagtg ggcggtcttg gcccggtcgg
     1561 tttcatggct gtcgcggatg agagcccgca tggcggcgca gccgtcgccg cgccgcgaca
     1621 ggacgttcag cagcatgccg tgcgacacct cgaagcgcga cgcaagccgc tcgggcgggg
     1681 cgccgatgag ccgcgtgaag gtcgcgtggt cccagttggc gaagttgtgc tccggcggct
     1741 tcctccggac gaacttcttc ccgtccttcg ccttctccgc cagccggagg ttctcgacga
     1801 cgtgctcggg ggcctgcgcc acgacgaacc cctcgtcgtc gaaccccttc cgtccggcgc
     1861 gcccggcgat ctgatggaag tcgcgcgccg acaggaccgc ggtcttctgc ccgtcgtact
     1921 tgcagagctt cgtgaacagc accgtccgga tcggcacgtt gatcccgacc ccgagggtgt
     1981 cggtgccgca gatgaccttg agcagcccgc cctgcgccag tcgctcgatc aggacgcggt
     2041 agcgcggcag gaggccggcg tggtgcacgc cgatgccctg cttgagccat ttcctgatcg
     2101 tcggaccata cgggctcgtg aagcggacgt cggc
//
LOCUS       DASRIQ010000549         8519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_986543, whole genome shotgun sequence.
ACCESSION   DASRIQ010000549 DASRIQ010000000
VERSION     DASRIQ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986543"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VFD06_13595"
     CDS             <1..1076
                     /locus_tag="VFD06_13595"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZN04612.1"
                     /translation="PAPAAGQEGQEAPAQQYVCPPCDMECDQRVFDTPGRCPACGAAL
                     VEKTSQQARDERPGAAILIFEGVQIIDYTGPYEVFGQGGYRVFTVAEAKRPLRTAMGM
                     TVTPEYSFADAPKADVLVVPGGDVSGPTGSAATITWVKTQSAATRQTLSVCNGAFILA
                     KAGLLDGLSATTFAGLIDDLKEAAPKTRIVSDQRFVDNGRIVTAAGLSSGIDGALHVI
                     EKLKGLGTAQATAIHMEYDWRPDSGYARAALADRLIPRVNLGDGSTLTMLHTRGDRDR
                     WDIAVAVQTPLAPADILAKVDQALAAGRWNKGKTETRGRGTASEWRRQGESVGSWTGR
                     LTLEPDGKEAGRYTLAMTIQRKG"
     gene            1355..2905
                     /locus_tag="VFD06_13600"
     CDS             1355..2905
                     /locus_tag="VFD06_13600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN04613.1"
                     /translation="MISARHRGIILVEPDINPIARRFSLPNIANYPPLPQVRLAGQLP
                     PGENVAIADLRIAGERRRLLERVRRDPPALVGISLTFTSNGDEAIGVAAAIRRLSPAT
                     CIVLGGTAPSEDPASFWVSAADLICHRSGDASFAALAVEVRETGRTPARFPGFFHRDG
                     GRWVLDPGPAPLPLASLRPNPWHLLPKRYWRWYFQGFRPTGMGQTSEGCPYDCTFCTV
                     WKTHGRRVSVASLANVQHDFDSLPRFVRSYFFADDIWMQASERQLRELYDPLLAWMAS
                     DFLPRHRDFRLTVETRTDLYLREEDRFYSWIRRGALKRILFGVEAVTDEQLRNFSKRN
                     TVDRNIEAIRRAAEAGAIVTAQFVIPCDATRAYFDEMVRFLKAHRGWIRTSNFTIATP
                     LPGTDLYEGSLRESPELADRSAISHPAFSLFTALTPTRLDVPEFYEQVARVFRAANQV
                     RFSLEAQQQLFAMVVKSPWLIRRLAKAPWALRALTSPGTFLDVHRLVQGDRLLEPRPA
                     SQRTPQFA"
     gene            3146..3367
                     /locus_tag="VFD06_13605"
     CDS             3146..3367
                     /locus_tag="VFD06_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04614.1"
                     /translation="MNSMKILAIVLIVGGLLGLVYGGFTYTKESHDLKLGKLEVSVKD
                     KKTINIPMWVGVGAVAAGAALLVFRPKLP"
     gene            3422..4984
                     /locus_tag="VFD06_13610"
     CDS             3422..4984
                     /locus_tag="VFD06_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04615.1"
                     /translation="MKNRWRAWSWRRRSLSVVAGALLLATGLLAVYVIGANLLLRTRL
                     LRALINHGPDKIWIDYSSAYSLRPGRVRVRDLRIRDRGVVTEWIISLDEARGSIRLTD
                     LLRKRFHTTRIRGKGLAVRVRNRLMAEEATPRRLSFLPPITGFPAPPIREPGERKPLP
                     TGREWTVRLDDIAVDAVREIWVNEYHYTGEATLTGSMLLHPRLRFEVFPTTLAVRSGT
                     LRFNEEPIAASVEARLDGLIHPYDLRQSSGGAVLKSMTGTGRAAGRIESLHVLNDMLD
                     IPPTLRLDDGTGTLAAELSLDRGSGHGALDFSAQGARAVMPDAVMTGEAEGRLRLARL
                     DLHGGLLDFAGSHLEVRKVMVKRGNETPWPWWGILEIPAADLRTGPPRVLYAHAALRA
                     QNAQPLYRLLNAKLPSWAEWLLKMEGVTATADVALGPSFADVKSLEARGGAFHILGRY
                     HREKGTNNAAFLIDAGPLVVGVGLRGGKSEVEIATPRTWFRDRLARQDTPSGLLQPKS
                     VDPAEEDRTLTQ"
     gene            5057..5944
                     /locus_tag="VFD06_13615"
     CDS             5057..5944
                     /locus_tag="VFD06_13615"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZN04616.1"
                     /translation="MKDAVGRFVRDGDLVVMEGFTHLIPFAAGHEIIRQERRDLTLAR
                     LTPDLIYDQLIAAGCVKKMIFSWAGNPGVGPLHAFRRAVEKGTPRPLEIEEYTHFGMV
                     LRFMAGASNLPFLPTRNYPGTDLTSVNPLFKKVRSPYSDEELWCVPPIHPDVTIVHAQ
                     RADEDGNTQVWGLYGVQKEAAFASRKVIVTVEEVVPASVIRSDPNRTIIPGIIVSAVV
                     PEPFGAHPSFVQGAYDRDNDFYVAWDAISREEAGLRKYLDEWVHGVPDRTAYTKKMAA
                     RREALQADRQMCEPVNYGF"
     gene            5944..6720
                     /locus_tag="VFD06_13620"
     CDS             5944..6720
                     /locus_tag="VFD06_13620"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004595063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZN04617.1"
                     /translation="MADYTTSELMVSRAAKELRNGDVVFVGIGVPSLAVNLAYRMHAP
                     DLCMIYESGAVGCVPTRLPISIGDPCLVTGSLAVVPMLDVFNLYLQGGKIDVGFLGGA
                     QVDRFGNINSTVIGDYRKPKVRLPGSGGACEIAALAKKVVITIANSKRTLPEKVDFVT
                     SPGYLRGGRERESLGLKGGPELVITDMGVYHFDPETREMVLESLHPGVTLETIRENTG
                     WPVKSAPSLKETPPPTAEEIRIVRDELDPQGIFLRKGAQG"
     gene            6717..>8519
                     /locus_tag="VFD06_13625"
     CDS             6717..>8519
                     /locus_tag="VFD06_13625"
                     /inference="COORDINATES: protein motif:HMM:NF005685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN04618.1"
                     /translation="MKKKAKKKTARRLSARQRSAITRRRSGPGGKGKRAGRRALPKAL
                     LASAAIAHATARAQASVRTELSQKSRLAERMTEAALNEAEEIGMLLDEEGDAEAEEDP
                     DSSGEADYFLKPQPIGPRHPRVRPAPGIETGVPPAARRLREASRKRRSPAAGRGGSAR
                     GTGPESAPSLLPQGAEHLFPRNLKLSYPVAVKAEGVFIEDASGKRYLDGCGGAVVCSI
                     GHGVKEIADVMTAQARRLAFAHSSQFVTREAMDLTSRIAALAPGDMKETGRVYLVSGG
                     SEAVETGLKLARQYHVERGRPGKHKVIGRWGSYHGSTLGALSVTGNAARRDLYLPMLP
                     PSPHIAPSFCYRCPLGLEFPECAVSCIDELETAIRQEGPDTVAAFIAEPVVGATLGAV
                     PTVKGYFERARALCDQYDMLLIADEVMTGVGRTGKNFGIDHYGVVPDLIVTGKGLSAG
                     YAPLGAVIAGDHVVEALARGRGYFEHGFTYSANPLSAAVGSAVLEYIEEHNLIEQAAT
                     LGTILGKRLGDLKKRHRIIGDVRGLGMMWGLEIVKNRSSKEPFPPALKVARRLYEASL
                     EEGLMIYPGTGTREGRDGDHCIIAPPFTITRSELD"
BASE COUNT         1352 a         2994 c         2927 g         1246 t
ORIGIN      
        1 ttcccgcccc ggccgccggc caggaggggc aggaggcgcc ggcgcagcag tacgtctgcc
       61 cgccgtgcga catggagtgc gatcagcggg tgttcgacac gccgggccgc tgccccgcgt
      121 gcggcgcggc gctggtcgag aagacgtccc agcaggcgcg ggacgagcgg ccgggcgcgg
      181 ccatcctgat cttcgagggg gttcagatca tcgattacac cgggccgtac gaggtgttcg
      241 gccagggagg ctaccgcgtg ttcaccgtcg cggaagcgaa gcggcccctc aggaccgcga
      301 tggggatgac cgtcaccccc gagtactcct tcgccgacgc gccgaaggcc gacgtgctcg
      361 tcgtcccggg cggggacgtg agcggcccga ccggcagcgc cgcgacgatc acctgggtca
      421 agacgcagag cgccgcgacc cggcagaccc tctcggtctg caacggcgcc ttcatcctgg
      481 cgaaagcggg actcctcgac gggctgagcg ccacgacctt tgcaggcctc atcgacgacc
      541 tcaaggaggc ggccccgaag acgcgcatcg tcagcgacca gcgcttcgtg gacaacggac
      601 gcatcgtcac cgcggccggc ttgtcgtcgg ggatcgacgg cgcgctgcac gtcatcgaga
      661 agctcaaagg gctggggacg gcacaggcga ccgccatcca catggagtac gactggcgcc
      721 ccgactccgg ctacgcgcgg gcggcgctcg ccgaccggct catcccgcgc gtgaatctcg
      781 gcgacggctc gaccctgacc atgctgcaca cgcgcggcga tcgcgatcgc tgggacatcg
      841 cggtcgcggt ccagaccccc ctcgccccgg ccgacatcct cgcgaaggtg gaccaggcgc
      901 tcgcggcggg gcgctggaac aaggggaaga ccgagaccag ggggagaggc accgccagcg
      961 aatggcgccg gcagggggag agcgtcggct cctggaccgg ccggctcacg ctcgagccgg
     1021 acggcaagga agcgggccgc tacacgctcg cgatgaccat ccagcgaaag ggctgatccg
     1081 gccgggaccc ggcgagggtc acgaacccgt caatcggggc atcgcctgaa tcccttgtcc
     1141 ggagcggcct gacagacgca acacccgccg cagcggggac agaaccggct cgagagccgg
     1201 ccaccgggct caagtgcgcc ctccgtcgcc acttccaggc cgggaggcgg gatccgacct
     1261 ggtgtccgga ggaatccccg ggcgaaacgg ggaaccggac ccgttcgccg accattcggc
     1321 gccgggcagg cagggatcga taattggagt actcatgatc tctgcccggc atcgtggaat
     1381 cattctcgtc gagcccgaca tcaatcccat cgcacgtcgt ttcagtcttc cgaacatcgc
     1441 gaactacccg cccctccccc aggtgcgcct cgccggccag ctcccgccgg gagagaacgt
     1501 ggcgatcgcg gatctccgca tcgcgggcga gcggcggcga ctgctcgagc gcgtccggcg
     1561 ggatccgccc gccctggtcg gcatctccct caccttcacg tcgaacggag acgaggcgat
     1621 cggggtggcc gcggcgatcc ggcgcctctc ccccgcgacc tgcatcgtcc tcggtgggac
     1681 cgccccctcc gaagaccccg cgtcgttctg ggtctcggcg gcggatctga tctgccaccg
     1741 gagcggcgac gcctcctttg cggctctggc cgtcgaggtg cgcgagacgg gccggacgcc
     1801 ggcacggttc cccgggttct tccaccgcga cggcgggcgc tgggtcctcg accccgggcc
     1861 ggcccccttg cccctggcga gcctccgacc caatccgtgg cacctgctgc cgaagcgata
     1921 ctggcgctgg tacttccagg ggtttcggcc caccggcatg ggtcagacga gcgaaggctg
     1981 cccttacgac tgcaccttct gcaccgtctg gaaaacccac ggccgcaggg tctcggtagc
     2041 ctccctcgcg aacgtccagc acgacttcga ctccctcccc cgcttcgtcc gctcctactt
     2101 cttcgcggac gacatctgga tgcaggccag cgagagacag cttcgggaac tctacgaccc
     2161 gctgctggcg tggatggcct ccgacttcct cccgcgccat cgcgatttcc ggctgacggt
     2221 cgagacgcgc accgacctgt atctgcgcga ggaggatcgc ttctattcct ggatccggcg
     2281 cggcgcgttg aagcggatcc tcttcggcgt ggaggcggtg acggacgagc agctccggaa
     2341 cttcagcaag cggaacacgg tcgacaggaa catcgaggcc atccgtcgtg ccgcggaggc
     2401 cggggccatc gtcacggccc agttcgtcat cccgtgcgac gcgacacgcg cctacttcga
     2461 cgagatggtc cggttcctga aagcccatcg gggctggatt cgcacgtcca acttcaccat
     2521 cgcgaccccg cttccgggca ccgacctcta cgagggctcc ctgagggagt cgcccgagct
     2581 cgccgaccgg agcgccatct cgcaccccgc gttctcgctc ttcacggccc tgaccccgac
     2641 ccgtctcgac gtccccgagt tctacgagca ggtggcacgc gtgttccgcg cggccaacca
     2701 ggtccgcttc agcctggagg cccagcaaca gctcttcgct atggtggtca agtccccatg
     2761 gctgattcgg cgcctcgcca aagcgccttg ggcgctccgc gcactgacca gccccggcac
     2821 cttcctcgac gtccaccggc tggtccaggg ggatcgactt ctcgagcccc ggcccgcgtc
     2881 ccagcgcacc cctcagttcg cctgagcacg gacgcgggcg cgtcggaagc ggatcgcggc
     2941 ggcctcacgc aggcctgggc gtggtcgaga cgcaccggat gcgattgatg gacaagctcg
     3001 acgtcagggt cttcctgacc ctctgtcgga aaagaagagt cgtcatgcgg gaacctgccg
     3061 cattgttgct tccgcaccgc tggactacaa agaaccgtcg cgtcacgccc cacgtgtcca
     3121 tcgggtgatc cactggaggg tacacatgaa ctcaatgaag atcctcgcca tcgtgctgat
     3181 cgtgggaggt ctcctcgggc tggtgtacgg cggattcacc tatacgaagg agagccacga
     3241 tctcaaactg gggaagctgg aggtgtcggt caaggacaag aagacgatca acatcccgat
     3301 gtgggtcggc gtgggagccg tcgcggccgg cgcggcgctg ctggtcttcc gtccgaagct
     3361 tccgtagctc cgaccgctgg actccgggga attcacgcgt gccggggaga gactcgaaac
     3421 ggtgaagaac agatggcgtg cctggtcgtg gaggcggcgg agcctctcgg tcgtcgcggg
     3481 tgcgctcctg ctggccaccg gcttgctcgc cgtctatgtc atcggcgcaa acctgctgct
     3541 tcggacgcga ttgctgcgcg ccctcatcaa ccatggcccg gacaagatct ggatcgacta
     3601 cagctccgcc tactccctcc gtccgggacg agtgcgggtg cgggacctgc gcatccggga
     3661 ccgcggtgtc gtcacggagt ggatcatttc gctcgacgag gcgcggggct ccatccgcct
     3721 caccgacctg ctgcggaaac gcttccacac cacccgcatc cggggaaagg ggctggcggt
     3781 ccgcgtccgg aaccgtttga tggctgagga ggcgacacct cgccgcctct ccttcctgcc
     3841 ccccatcacc gggtttcccg ctccgccgat ccgcgagccc ggcgagagga agccgctccc
     3901 caccggacgc gagtggaccg tccggctcga cgacatcgcc gtcgacgcgg tcagggagat
     3961 ctgggtgaac gaataccact acaccgggga ggccacgctg acggggagca tgttgctgca
     4021 cccccggctc cggttcgagg tcttcccgac cacgctcgca gtcaggagcg gcacgctgcg
     4081 cttcaacgag gagccgatcg ccgcctccgt ggaggcgcgt ctcgacggcc tcatccaccc
     4141 ctatgacctg cgccagtcgt ccggaggggc ggtcctgaaa tcgatgaccg ggacggggcg
     4201 ggccgcgggg aggatcgaga gcctccatgt cctcaacgac atgctggaca tcccgccgac
     4261 gctccggctg gacgacggga ccgggaccct ggcggccgag ctctcgctcg accgcggctc
     4321 cggccatgga gcgctggatt tctcggcgca gggggccaga gccgtgatgc ccgacgcggt
     4381 catgacggga gaggccgagg gacggctgcg tctcgcccgg ctcgacctcc acggggggct
     4441 gctggacttc gcgggaagtc acctcgaggt gcgcaaggtg atggtgaagc ggggcaacga
     4501 gacaccctgg ccctggtggg ggatcctcga gatcccggcg gcagatctgc gcaccggccc
     4561 gccgcgcgtc ctctatgcgc acgccgccct gcgcgcgcag aacgcgcagc ccctgtaccg
     4621 gctgctgaac gcgaagcttc cctcctgggc ggagtggctg ctgaagatgg agggggtgac
     4681 cgcaaccgcc gatgtggcgc tcggaccatc gttcgcggac gtgaagtcgc tcgaggcgcg
     4741 cggcggtgcc tttcacatcc tggggcggta ccacagggag aaagggacga acaacgcagc
     4801 gttcctgatc gacgcggggc ctctcgtggt cggcgtcggg ctccgtggcg gaaagtccga
     4861 ggtcgagatc gccaccccga ggacctggtt tcgcgaccgc ctggcgcgcc aggacactcc
     4921 ctcgggcctc ctccaaccga aatccgtgga tcctgcggag gaagaccgca ccctcacgca
     4981 atgagcctcc ggggccgcgc ccggcggggc gcggaccccg tgttatcatc cgcctcccgt
     5041 gtccaagatc atgaccatga aagacgccgt cgggcgcttc gtgcgtgacg gcgacctcgt
     5101 ggtcatggag gggttcaccc acctcatccc gttcgccgcc gggcacgaaa tcatccgaca
     5161 ggagcgccgt gacctgacgc tggcgcgcct gacgccggac ctcatctacg accagctcat
     5221 cgccgccggg tgcgtgaaga agatgatctt ctcgtgggcc ggtaacccgg gggtcgggcc
     5281 gctgcacgcc ttccggcgcg cggtcgagaa ggggaccccg cggccgctcg agatcgagga
     5341 gtacacgcac ttcgggatgg tgctgcgttt catggccggc gcctcgaacc tgccgttcct
     5401 gccgacgcgc aactaccccg gcaccgacct gacctcggtc aatcccctct tcaagaaggt
     5461 gcgctcgccc tactccgacg aggagctctg gtgcgtgccg ccgatccacc cggacgtgac
     5521 catcgtgcac gcgcagcggg ccgacgagga cggcaacacg caggtctggg ggctgtacgg
     5581 cgtgcagaag gaggcggcgt tcgcgagccg gaaggtgatc gtcaccgtcg aggaggtcgt
     5641 gccggcgtcg gtcatccgtt ccgatccgaa ccgcaccatc atcccgggga tcatcgtctc
     5701 cgccgtcgtg ccggagccgt tcggcgccca cccgtcgttc gtgcaggggg cgtacgaccg
     5761 ggacaacgac ttctacgtcg cgtgggacgc gatctcccgc gaggaggcgg gcctccggaa
     5821 atacctcgac gagtgggtgc acggggtgcc cgaccggacc gcctatacaa agaagatggc
     5881 ggcgcggcgc gaggcgctcc aggccgaccg gcagatgtgc gagccggtca actacgggtt
     5941 ctgatggccg actacacgac cagcgagctg atggtgtcgc gcgccgccaa ggagctgagg
     6001 aacggcgacg tcgtcttcgt cggcatcggc gtgccgtcgc tcgcggtcaa cctcgcctac
     6061 cgcatgcacg ccccggacct ctgcatgatc tacgagtcgg gggcggtggg atgcgtgccg
     6121 acgcggctgc cgatctcgat cggcgacccg tgcctggtga ccgggtcgct ggcggtcgtg
     6181 ccgatgctcg acgtcttcaa tctctacctg cagggtggga agatcgacgt cggcttcctc
     6241 ggcggcgccc aggtggaccg cttcggcaac atcaactcga ccgtcatcgg cgactaccgc
     6301 aagcccaagg tccggcttcc gggaagcggc ggcgcgtgcg agatcgccgc cctcgcgaag
     6361 aaggtggtca tcaccatcgc gaactcgaag cgcacccttc ccgagaaggt cgatttcgtc
     6421 acgagccccg ggtacctgcg cggcgggcgc gagcgcgagt cgctcggcct gaaggggggg
     6481 ccggagctgg tgatcaccga catgggggtg taccacttcg acccggagac ccgcgagatg
     6541 gtcctcgagt cgcttcaccc cggcgtcacc ctggagacga tccgcgagaa caccggctgg
     6601 ccggtgaagt cggcgccgag cctgaaggaa acgccgccgc cgaccgccga ggagatccgc
     6661 atcgtgcggg acgagctcga tccgcagggg atcttcctgc gcaagggagc gcagggatga
     6721 agaagaaggc gaagaagaag acggcgcggc gcctgtccgc gcggcagcgt tcggccatca
     6781 cccgccgccg gtcgggcccc ggcgggaaag ggaagcgcgc cggccggcgt gcgttgccga
     6841 aggcgctcct ggcctcggcc gcgatcgccc acgccaccgc ccgcgcccag gcgagcgtgc
     6901 gcaccgagct gagccagaag tcccgcctcg cggagcggat gacggaagcg gcgctcaacg
     6961 aggcggagga gatcggcatg ctcctcgacg aggaggggga cgccgaggcg gaggaggacc
     7021 ccgactcatc cggcgaggcg gactatttcc tcaagccgca gccgatcggc ccgcggcacc
     7081 cgcgcgtccg accggctccc ggcatcgaga ccggcgtccc tcccgccgcg cgacgattgc
     7141 gcgaagcgtc gcggaagcgg cgctccccgg ctgcggggcg cggcgggtcg gcccgcggca
     7201 ccggcccgga atccgctccc tccctcctgc cccagggggc ggagcacctg ttcccccgga
     7261 atctgaagct ctcctatccg gtcgcggtca aggcggaggg ggtcttcatc gaggacgcct
     7321 cggggaagcg ctacctcgac ggctgcggcg gcgcggtcgt ctgctccatc ggccacggcg
     7381 tcaaggagat cgccgacgtg atgacggcgc aggcgcggcg gctggccttc gcccactcgt
     7441 cgcagttcgt cacccgcgag gcgatggacc tgacgtcgcg catcgcggcg ctggcccccg
     7501 gggacatgaa ggagaccggg cgcgtctacc tcgtctccgg cggcagcgag gcggtggaga
     7561 ccgggctgaa gctggcgcgg cagtaccacg tcgagcgcgg ccgcccgggc aagcacaagg
     7621 tgatcgggcg atgggggtcg taccacggct cgacgctcgg ggcgctgtcg gtcaccggca
     7681 acgcggcgcg gcgcgacctc tacctgccga tgctgccgcc gtcgccgcac atcgcgccgt
     7741 cgttctgcta ccgctgcccg ctcggcctcg agttcccgga gtgcgccgtc tcctgcatcg
     7801 acgagctgga gaccgccatc cggcaggaag ggccggacac cgtcgccgcc ttcatcgccg
     7861 agccggtggt cggggccacg ctcggggcgg tgccgaccgt gaaggggtat ttcgagcggg
     7921 cgcgcgccct ctgcgaccag tacgacatgc tgctcatcgc cgacgaggtg atgaccggcg
     7981 tcggtcgcac cgggaagaac ttcggcatcg atcactacgg cgtcgtgccg gacctgatcg
     8041 tcaccggcaa ggggctgtcg gccggctacg cgccgctcgg ggcggtgatc gccggcgacc
     8101 acgtcgtcga ggcgctggcg cgcggccgcg gctatttcga gcacggcttc acctacagcg
     8161 cgaaccccct gtcggccgcg gtcggcagcg ccgtcctcga gtacatcgag gagcacaacc
     8221 tcatcgagca ggccgcgact ctggggacga tcctcgggaa gcgcctcggg gatctcaaga
     8281 agcgccaccg catcatcggc gacgtccgcg gcctcgggat gatgtggggg ctcgagatcg
     8341 tgaagaaccg ctcctcgaag gagccgttcc cgccggccct caaggtggcg cgccggttgt
     8401 acgaggcgag cctcgaggag gggctgatga tctaccccgg caccggcacc cgcgagggac
     8461 gcgacggcga ccactgcatc atcgctccgc cgttcaccat cacccgctcc gagctggac
//
LOCUS       DASRIQ010000550         5048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2838579, whole genome shotgun sequence.
ACCESSION   DASRIQ010000550 DASRIQ010000000
VERSION     DASRIQ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2838579"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFD06_13630"
     CDS             <1..273
                     /locus_tag="VFD06_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) small subunit"
                     /protein_id="HZN04619.1"
                     /translation="KFGHRAHNQPCVDTRTGRCYQTSQNHGFAVDGASLPRGWKPWFV
                     NANDGTNEGLRHERAPIRSVQFHPEAAPGPAGTEWILDEFLAEARR"
     gene            270..3623
                     /gene="carB"
                     /locus_tag="VFD06_13635"
     CDS             270..3623
                     /gene="carB"
                     /locus_tag="VFD06_13635"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HZN04620.1"
                     /translation="MTRKAGRRQRVLLLGSGALKIGEAGEFDYSGSQAIKALKEEGVE
                     VWLINPNIATIQTSPGLADRIFLLPVEPGFVTRVIAEHRPDGLLLSFGGQTALNCGLA
                     LHRRGVLKRYGVKVYGSPISAIEMTEDRQRFAQELRRIGIQVPRSAAARTLSQALRIA
                     RRIGYPVMMRTGFTLGGRGSGIVRGEAELRARATEAFTSSPQILIEEDLTGWKEIEYE
                     IVRDRADNIITVCNMENVDPLGIHTGESIVVAPSQTLTDREYFMLRDLSFQAIRHFGI
                     VGECNIQFALDPRSDAYRVIEVNARLSRSSALASKATGYPLARIAAKLALGRLLPDLK
                     NSMNDTTTACFEPALDYLVVKMPKWDLEKFRGASHFIGSEMKSVGEVMAIGRSFEEAL
                     QKGCRMLGDGIVGLVDNDFNIRQSLADLAVPTPRRVFALAEAMRRGKSVAELARQTGI
                     DAWFLAKMARIVRVRAALRGARAGLTDELLREAKEAGFSDAQIARATGRKEEAVAAAR
                     RSAGIVPAVKQIDTLAAEWPVRTNYLYTTYQGREDDTSPGAADNRVLILGSGAYRIGS
                     SVEFDWCCVSAAAALRRLGYAPVVVNCNPETVSTDYDMGHTLYFEELTFERLRDIWDK
                     ERPRGILLAFGGQIPNNLALACGRAGLPVLGTQPVDIDRAEDRHKFSTLLDRLGVPQP
                     PWAEVTSRREAVRFAARVGYPVLVRPSYVLSGAAMRIAADGSQLTAALRGAAEVSRQH
                     PVVISKFIQGAKELEADAVASGGRLLAVAVTEHVENAGVHSGDATVVFPPQKIYLETV
                     RRIRDITARLARALRITGPFNVQYLAIDNEVMVIECNLRASRSFPFISKVSGVDFVDL
                     ATRAMMGEPVQPVPESLLDLDYVGVKAPQFSFSRIRGADPALRVEMASTGEVACLGHD
                     LPEAFLKAILSAGFRLPEKKRVLLSIGSEADKVRFLPSARLLRDLGYTLYATRGTSKF
                     LRHHGIAHTRLYKIRERRAPSLLDFISPERLDLILNVPLGLDRAELTDGYIIRRRAID
                     YGVPLITNLQLAELLVKALATKTLDDLRPLSYEAYVPGSDPARAEGGAIPAPLRRRAT
                     GEPLKRLPARGRRASAPAPPALPLQADLA"
     gene            complement(3605..4759)
                     /locus_tag="VFD06_13640"
     CDS             complement(3605..4759)
                     /locus_tag="VFD06_13640"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZN04621.1"
                     /translation="MIRKGLEGSLFTMSLPDLLQWASRGQKTGTLLVRNERVAKKIHF
                     LGGLIIGSSSTDPGDYLGQVLLSEGLITEEHLREAMDLQARTKVMLGRLLVQRGVVTE
                     ERVAAVLRYKAEETIYSLFLWADGTFEFHEGELPPSEQVRFKVQVEQVLLEGVRRYDT
                     SRKIREVLRDNDVVLQATDVPLPADIGAKAFPRRIYQLVDGRRTIADIILEAHSSEFN
                     VCQVLYIMVLRGYAALAPSSRPAGAPAASAPPALEALLAEARTCLGRGEAEEALALLE
                     AAHEAGLRAAELQALTHEAERHFLERAYRHYLPPGKVPVLKRPLESLVDESLSPAEMF
                     LVSRVNGAWDLRSIVSISPLREVEALRALKRLRERGVIDLVDPPAQARSA"
BASE COUNT          755 a         1794 c         1755 g          744 t
ORIGIN      
        1 aagttcgggc accgggcgca caaccagccg tgtgtcgaca cgcgcacggg ccgctgctac
       61 cagaccagcc agaaccacgg cttcgccgtg gacggcgcga gcctcccgcg gggctggaag
      121 ccctggttcg tcaacgccaa cgacgggacg aacgaggggc tgcgccacga gcgcgccccg
      181 atccggtcgg tgcagttcca cccggaggcg gcgcccggcc cggccgggac cgagtggatc
      241 ctcgacgagt tcctggcgga ggcgcggcga tgacgcgcaa ggccggccgc cggcagcggg
      301 tgctgctcct cgggtccggg gcgctgaaga tcggcgaggc gggggagttc gactactccg
      361 gctcgcaggc gatcaaggcg ctcaaggagg agggggtcga ggtctggctc atcaacccca
      421 acatcgccac catccagacg agccccggcc tcgcggatcg catcttcctg ctcccggtgg
      481 agccgggctt cgtgacccgg gtcatcgccg agcaccgccc ggacgggctg ctcctctcct
      541 tcggcggcca gaccgccctc aactgcgggc tggcgctcca ccgccgcggg gtgctgaagc
      601 gctacggcgt gaaggtctac ggcagcccga tctccgccat cgagatgacc gaggaccggc
      661 agcgattcgc gcaggagctg cgccgcatcg gcatccaggt gccgcggagc gcggcggccc
      721 gcacgctgtc ccaggcgctg cggatcgccc ggcggatcgg ctacccggtc atgatgcgca
      781 ccggcttcac cctcggcggg cgcggcagcg gcatcgtccg gggcgaggcg gagctgcgcg
      841 cccgcgccac cgaggcgttc acctcctcgc cgcagatcct catcgaggag gacctgaccg
      901 gctggaagga gatcgagtac gagatcgtcc gcgaccgggc cgacaacatc atcacggtgt
      961 gcaacatgga gaacgtcgac cccctcggca tccacaccgg cgagtcgatc gtcgtggcgc
     1021 cgtcgcagac gctgaccgat cgcgagtact tcatgctgcg cgacctgtcg ttccaggcga
     1081 tccggcactt cgggatcgtc ggcgagtgca acatccagtt cgccctggat ccgcgctccg
     1141 acgcctaccg ggtgatcgag gtgaacgcgc ggctgtcgcg ttcgtcggcg ctggccagca
     1201 aggcgaccgg gtacccgctg gcgcgcatcg ccgccaagct cgcgctcggc cggctgctgc
     1261 cggacctgaa gaactccatg aacgacacga ccaccgcctg tttcgagccg gcgctcgact
     1321 acctcgtggt gaagatgccg aaatgggacc tggagaagtt ccgcggagcc tcccacttca
     1381 tcggcagcga gatgaagtcg gtgggtgagg tcatggcgat cggccgctcc ttcgaggagg
     1441 cgctgcagaa gggctgccgg atgctgggcg acggcatcgt ggggctcgtg gacaacgact
     1501 tcaacatccg ccagtcgctc gccgatctcg ccgttccgac gccgcggcgc gtgttcgccc
     1561 tggccgaagc gatgcgccgc ggcaagagcg tcgccgagct cgcccgccag accggcatcg
     1621 acgcgtggtt cctcgccaag atggcccgca tcgtccgggt tcgcgccgct ctccgcggcg
     1681 ctcgggcggg gctcaccgac gagttgttgc gcgaggcgaa ggaggccggg ttctcggacg
     1741 cgcaaatcgc gcgggccacc ggaaggaaag aggaggcggt cgcggcggcg cgccggtcgg
     1801 cgggcatcgt ccccgccgtg aagcagatcg acacgctggc ggccgagtgg ccggtgcgga
     1861 ccaactacct ctatacgacc taccaggggc gcgaggacga cacgtcgccc ggagcggccg
     1921 acaaccgggt gctcatcctc ggatccggcg cctatcggat cggctcgtcg gtcgaattcg
     1981 actggtgctg cgtctcggcg gcggcggcgc tccggcgcct cggctacgcc ccggtcgtcg
     2041 tcaactgcaa tcccgagacg gtctccaccg actacgacat gggtcacacc ctctacttcg
     2101 aggagctgac cttcgagcgg ctgcgcgaca tctgggacaa ggagcggccg cgcgggatcc
     2161 tcctcgcctt cggcgggcag atcccgaaca acctggcgct cgcctgcggg agggccgggc
     2221 tcccggtcct cggcacgcag cccgtggaca tcgaccgggc ggaggatcgg cacaagttca
     2281 gcacgctcct cgatcgcctc ggcgtgcccc agcccccctg ggcggaggtg acgtcgcggc
     2341 gcgaggcggt gcgcttcgcg gcacgcgtgg gctacccggt gctggtgcgg ccttcttatg
     2401 tgctgtccgg ggccgcgatg cggatcgccg ccgatgggtc gcagctgacc gccgccctgc
     2461 gcggcgccgc cgaggtctcg cgccagcacc ccgtcgtcat cagcaagttc atccaggggg
     2521 ccaaggagct cgaggccgac gcggtcgcct ccggaggccg tctcctggcg gtggcggtca
     2581 ccgagcacgt cgagaacgcc ggggtgcact cgggggacgc caccgtggtc ttcccgccgc
     2641 agaagatcta cctcgagacg gtgcgccgca tccgcgacat caccgcgcgg ctggcgcgcg
     2701 cgctgcggat caccggcccg ttcaacgtgc agtacctggc gatcgacaac gaggtgatgg
     2761 tcatcgagtg caacctgcgc gcttcgcgct ccttcccgtt catctccaag gtgagcggcg
     2821 tcgacttcgt cgatctcgcg acgcgcgcga tgatggggga gccggtgcag ccggtgccgg
     2881 agtcgctcct cgacctcgat tacgtgggcg tcaaggcgcc gcagttctcg ttctcgcgca
     2941 tccgtggcgc cgacccggcg ctgcgggtcg agatggcctc gaccggggag gtcgcctgcc
     3001 tgggacacga cctgccggag gcgttcctca aggcgatcct gtcggcgggg ttccggcttc
     3061 cggagaagaa gcgggtcctc ctgtcgatcg ggtcggaggc ggacaaggtg cgcttcctgc
     3121 cgtcggcccg cctgctgcgc gacctcggct acacgctgta cgcgacgcgt ggcacctcga
     3181 agtttcttcg ccaccacggg atcgcgcaca cgcgcctcta caagatccgc gagcgccgcg
     3241 cgccgtcgct gctcgacttc atctcgccgg agcgcctcga cctcatcctc aacgtgccgc
     3301 tcgggctcga tcgggccgag ctgaccgacg gctacatcat ccggcgtcgc gccatcgact
     3361 atggcgtgcc gctgatcacg aacctccagc tggccgagct gctggtcaag gcgctcgcca
     3421 cgaagacgct cgacgacctg cgcccgctgt cgtacgaggc ctacgttccg gggagcgatc
     3481 cggcgcgcgc cgaagggggg gcgattccgg cgccgttgcg tcgccgcgca acgggagagc
     3541 cgctgaagcg cctccccgcg cgcggcaggc gggcctcggc cccggcgccg ccggcgctgc
     3601 cgcttcaggc cgatctggcc tgagccggcg gatcgacgag gtcgatcacc ccgcgctcgc
     3661 gcagccgttt gagggcccgc agcgcctcca cctcgcgcag cgggctgatg ctcacgatgg
     3721 agcgcaggtc ccacgctccg ttgacccggc tgacgaggaa catctccgcc gggctcagcg
     3781 attcgtccac gagcgactcg agcggccgct tcaggaccgg aaccttcccc ggcggcagat
     3841 agtgtcggta ggcgcgctcg aggaagtggc gctccgcctc gtgcgtcaga gcctggagct
     3901 ccgccgcccg cagccccgcc tcgtgcgccg cctcgaggag cgccagggcc tcctccgcct
     3961 cgccgcgccc gaggcatgtc cgcgcctccg ccagcagcgc ctcgagcgcc ggcggggctg
     4021 aagcggcggg cgcgccggcc ggccgcgacg acggtgcgag ggcggcgtag ccgcgcagca
     4081 ccatgatgta aagcacctgg cagacgttga actccgagga gtgcgcctcg aggatgatgt
     4141 cggcgatggt gcgccggccg tcgaccagct ggtagatgcg ccgcgggaac gccttggcgc
     4201 cgatgtcggc cggcagcgga acgtccgtcg cctgaaggac gacgtcgttg tcgcggagga
     4261 cctcgcggat cttgcgcgag gtgtcgtagc gccgcacgcc ttccagcagc acctgctcga
     4321 cctgcacctt gaagcggacc tgctcgctgg gcggcagctc accctcgtgg aactcgaatg
     4381 tcccgtccgc ccacagaaag agggagtaaa tcgtctcctc ggccttgtac cgcagcaccg
     4441 ccgcgacccg ctcctcggtc acgacgccgc gctgcacgag gaggcggccc agcatcacct
     4501 tcgtgcgggc ctggagatcc atcgcctcgc gaaggtgctc ctccgtgatc agcccctcgc
     4561 tgagcagcac ctgcccgagg taatcgcccg gatcggtgct cgacgagccg atgatgaggc
     4621 ctcccaggaa gtggatcttc ttcgcgacgc gctcgttccg gaccagcagg gtgccggtct
     4681 tctgtccccg gctcgcccac tgcagcaggt cgggcaggga catggtgaag agcgatcctt
     4741 cgaggccttt gcggatcatg cgtttgcggc gcgcattccg agccggcgtc atcgatttga
     4801 cgaacgatcg gccggaatgc ccccgggcaa tataggcacc tcggtcgcct gcgggcaacg
     4861 cgaccagccg ccacggagcc gccgtgcgat gtcaccgacc ttccaaggaa ggagtagaat
     4921 cgcgaaccgt gacactcgcc aaggggagcg cgcggacggc cgcccgcatg cgcgaaacgt
     4981 acaggatcgg ccgccgatga accatcggga ttgagcggtc gtccgagttc gatgccccgg
     5041 cgtgttct
//
LOCUS       DASRIQ010000551         4286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3091641, whole genome shotgun sequence.
ACCESSION   DASRIQ010000551 DASRIQ010000000
VERSION     DASRIQ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3091641"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFD06_13645"
     CDS             complement(<1..1115)
                     /locus_tag="VFD06_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN04622.1"
                     /translation="MIERIIAFSARNRVIVLILAAVAVGASIVAMREIPLDAIPDLSD
                     TQVIVYSRWDRSPDVIEDQVTYPIVTALLGAPRVKAIRGFSDFGYSYVYIIFQDGTDL
                     YWARSRTLEYLSKILPRLPEGVRTELGPDATGVGWVYQYALVDKTGRHDLAEMRSFQD
                     WSLRYWLQSVPGVAEVASIGGFQKQYQVEVDPEALLGYRIPLERVLENIRRGNNDVGG
                     RLVEVAGAEYMVRGRGYAKSIADIESIVVGDDGHGTPVLVRDVARVVLGPDIRRGVVD
                     LDGVGDAVGGIVVMRSGENALNVIERVKEKLASVRGALPQGVEIVPTYDRSELIVRSI
                     DTLKHELLLEMLIVSAVILIFLWHSPSSIVPIITIPVS"
     gene            complement(1112..2440)
                     /locus_tag="VFD06_13650"
     CDS             complement(1112..2440)
                     /locus_tag="VFD06_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN04623.1"
                     /translation="MTSGYRRLLTLAGIAALLAVGAAGGILWQTRRDDARPVATARDL
                     YHCPMHPTVVADRPGDCPICGMRLVRATVAPAGGGSAPDEGAAPVEGQAGVTIGNRKQ
                     QLINVRTVAASRAPFVRTLRVVGRVVPDETRLHHLHTKVGGWVEMLHLTSTGAKVEQG
                     APLLDLYSPELVASQEEYMLALRSRAESDQGALPEVARRADDLAQSARRRLLLFDMTP
                     QQIEELEQRGEPRRTVTIYSPISGYVIARNVTHGEKISPDMALFDIANLARVWVMASV
                     YEYELPFVQPGQAATTTLASLPGEPLSGRVDIIYPTLDEATRTAQVRLEFDNPRLQLK
                     PGMYAEVQIRSELGSRLSIPASAVVESGTRSIVFVEQGGGYFAPREVRIGLRLPDTME
                     VIAGLAENEKVVASGTFFVDSESKLKAALESAAAGRTSPPAQAPPGPRKP"
     gene            complement(2437..3759)
                     /locus_tag="VFD06_13655"
     CDS             complement(2437..3759)
                     /locus_tag="VFD06_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN04624.1"
                     /translation="MRRSIALFLFLPGICLAAESPPAPSLADLQREAAERSPSVRAAA
                     ARLDAAQRAPARLDVPPDPEISLTYLNDGVSSFTLGESEFSYLGLTWTQEVPYPGKTK
                     RAASVAEADAARAAIALRQERLAARAAVGQSYAEMFRIDQAAAILKESASILDVLARA
                     ARSRYEVGAGIQESVLKAETEILRLQAETARLEQDRAAAVAALAATLGRDAVSDPFGT
                     VTTLPPVTVPDDADDMAAAAIASSAMVVGARAAARRGEAAVEAARYESKPDLFWSAGY
                     QNRGDLDPMVMGMFGVRLPIHAERRQQAAALEAEDELRAARHDLAAAELRASAEARAA
                     WARARRADRLVELYEKGVVVQARATVASAQASYGVGRIDFLDLLHDLTVLLGARIEAA
                     TQQAERMQAVAALEALTGRDLIAGDSELDPGADAPGGNADARAVGGGR"
     gene            complement(3853..4215)
                     /locus_tag="VFD06_13660"
     CDS             complement(3853..4215)
                     /locus_tag="VFD06_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04625.1"
                     /translation="MSRFVWVGVLLTVLLAQAAPAASCLTGQAPSMPCCKPGSDCAAD
                     GLNVPSCCGLDAPVDRQEDADRHHGASVPSPDRNAVMVPNAPAADIVVLCGSCEAAGS
                     PPRDPGIPIYLRLGALLR"
BASE COUNT          636 a         1458 c         1602 g          590 t
ORIGIN      
        1 gagaccggga tggtgatgat cgggacgatc gacgaagggc tgtgccagag gaagatcagg
       61 atgacggcgc tgacgatgag catttcgagg agtagctcgt gcttcagcgt gtcgatcgag
      121 cggacgatga gctcggagcg atcgtaggtg gggacgatct cgacaccctg gggcagcgca
      181 ccgcgcaccg aggcgagctt ctctttgacc cgctcgatga cgttgagggc gttctcgccg
      241 gagcgcatga cgacgatccc gcccacggcg tcgccgacgc cgtcgagatc gacgacgcca
      301 cggcggatgt cggggccgag gacgacgcgc gccacgtcgc gcaccaggac cggcgtgccg
      361 tgcccgtcgt cgccaacgac gatcgactcg atgtcggcga tcgacttggc atagccgcgg
      421 ccgcgcacca tgtactccgc cccggccacc tcgacgagcc gcccgccgac gtcgttgtta
      481 ccgcgccgga tgttctccag cacccgctcg agggggatgc ggtagccgag gagcgcctcc
      541 ggatccacct cgacctggta ctgcttctgg aatccgccga tcgacgcgac ctccgccacg
      601 ccggggacgc tctgcagcca gtagcgcagc gaccagtcct ggaacgagcg catctcggcg
      661 agatcgtggc ggccggtctt gtcgacgagg gcgtactggt agacccagcc gacgccggtc
      721 gcgtcggggc ccagctcggt gcgcaccccc tcgggcaggc gcggcaggat cttgctcagg
      781 tactcgaggg tgcggctgcg cgcccagtac aggtcggtcc cgtcctggaa gatgatgtag
      841 acgtacgagt agccgaaatc cgagaagccg cggatcgcct tgacgcgcgg cgcaccgagc
      901 agcgccgtga cgatcgggta ggtgacctga tcctcgatga cgtcggggct gcggtcccac
      961 cgggaataga cgatcacctg cgtgtcggac aggtccggga tggcgtcgag agggatttcc
     1021 ctcatcgcca cgatcgaggc tccgaccgcc accgccgcca ggatcaggac gatgacgcgg
     1081 ttgcgtgccg agaacgcgat gatccgctcg atcatggctt ccggggcccc ggcggcgcct
     1141 gcgccggagg ggaggtccgg ccggcggccg ccgattcgag cgcggccttg agcttcgact
     1201 ccgaatcgac gaagaacgtg ccggatgcga cgaccttctc gttctcggcg agccccgcga
     1261 tcacctccat ggtgtccggg aggcggagcc cgatgcgcac ttcgcggggg gcgaagtagc
     1321 cccccccctg ctcgacgaag acgatgctgc gggtgccgga ctcgacgacg gcggaggcgg
     1381 ggatgctgag ccgcgacccg agttcgctcc ggatctgcac ctcggcgtac atcccgggct
     1441 tgagctgcag gcgcgggttg tcgaactcca gccgcacctg cgccgtgcgc gtcgcctcgt
     1501 cgagggtcgg gtagatgatg tccacccgcc ccgacagcgg ctcgccggga agggacgcga
     1561 gggtggtggt cgccgcctgc ccgggttgga cgaacggcag ctcgtattcg tacaccgacg
     1621 ccatgaccca gacgcgggcg aggttggcga tgtcgaacag cgccatgtcg gggctgatct
     1681 tctcgccgtg cgtgacgttc cgggcgatga catagccgga gatgggggag tagatggtga
     1741 cggtgcggcg cggctcgccg cgctgctcca gctcctcgat ctgctgcggc gtcatgtcga
     1801 acaggagcag gcgccgccgg gcgctctgcg cgagatcgtc ggcgcggcgc gccacctccg
     1861 gcagggcccc ctggtcggac tcggcgcgcg agcgcagcgc cagcatgtat tcctcctggc
     1921 tcgccaccag ctcgggactg tagaggtcga ggagcggcgc cccctgctcc accttggcac
     1981 cggtcgaggt gaggtggagc atctccaccc agccgccgac cttcgtgtgc aggtgatgca
     2041 ggcgggtctc gtccgggacg acgcgcccga cgacgcggag cgtgcgcacg aacggcgccc
     2101 gcgacgcggc gacggtgcgc acgttgatga gctgctgctt gcggttcccg atcgtgaccc
     2161 cggcctggcc ttcgaccggt gccgccccct cgtccggagc cgatccgccg ccggcgggtg
     2221 cgaccgtcgc gcgcaccaga cgcatgccac agatcgggca gtcgccggga cggtccgcga
     2281 ccacggtggg gtgcatcggg cagtggtaca gatcccgggc cgtggcgacg gggcgggcgt
     2341 catcgcgccg ggtttgccag agaatgccgc cggccgcgcc gacggcgagg agggcggcga
     2401 tcccggcgag ggtgaggagg cgccggtatc ccgaggtcat cgtccacccc ccaccgctcg
     2461 cgcgtcggcg ttgccgcccg gggcgtccgc gccgggatcg agctcggagt cgccggcgat
     2521 gaggtcgcgg ccggtgagcg cttcgagcgc cgcgaccgcc tgcatccgct ccgcctgctg
     2581 cgtcgccgcc tcgatccggg cgccgagcag gaccgtgagg tcgtggagca gatcgaggaa
     2641 gtcgatgcgg ccgacgccgt acgacgcctg cgccgaggcg accgtggcgc gcgcctgcac
     2701 gacgacccct ttctcgtaca gctcgacgag acggtcggcg cgccgggcgc gggcccaggc
     2761 ggcgcgcgcc tcggcggagg cgcgcagctc ggcggcggcg agatcgtggc gcgccgcgcg
     2821 cagctcgtcc tccgcctcga gcgccgccgc ctgctgccgg cgctcggcgt ggatcggcag
     2881 cctcacgccg aacattccca tcaccatcgg atcgaggtca ccgcggttct ggtagccggc
     2941 cgaccagaag agatccggct tcgattcgta gcgcgccgcc tcgacggcgg cctccccgcg
     3001 gcgggcggcg gcgcgcgccc ccacgaccat cgccgaggag gcgatcgccg cggcggccat
     3061 gtcgtcggcg tcgtcgggga ccgtcaccgg gggaagcgtc gtgacagtcc cgaacgggtc
     3121 ggagacggcg tcgcggccga gggtcgccgc gagcgcggcc acggcggcgg cgcgatcctg
     3181 ctcgagccgt gcggtctccg cctgcagtct caggatctcg gtctccgcct tgaggacgct
     3241 ctcctggatg ccggcgccga cctcgtagcg gctgcgcgcc gcgcgggcca ggacatcgag
     3301 gatcgaggcc gactccttga gaatcgccgc cgcctggtcg atgcggaaca tctcggcgta
     3361 cgactggccg acggcggcgc gggccgcgag gcgctcctgg cgcagcgcga tcgccgcccg
     3421 ggcggcgtcc gcctcggcga cgctggccgc gcgtttcgtc ttccccgggt acggaacctc
     3481 ctgcgtccag gtcaggccga ggtaggagaa ctcgctctca ccgagcgtga agctcgacac
     3541 cccgtcgttg aggtaggtca ggctgatctc cggatcgggg gggacgtcga ggcgggccgg
     3601 ggcgcgctgg gccgcgtcga gtcgtgccgc cgcggcgcgc acggagggac tgcgctcggc
     3661 cgcctcgcgc tgcaggtcgg cgagggaggg ggcgggcggg gattcggcgg cgaggcagat
     3721 cccgggaagg aacaggaaaa gggcgatgga gcgacgcatg gacacctcga tcgcgcccga
     3781 cgccaggcgg ggcgcgacac cactcgactc ggtcttgaaa aaagcgggag acagccgatc
     3841 ccggggccgg gatcagcgca ggagggcgcc cagtctcaga tagatcggga tgccggggtc
     3901 gcgcggcggg gagccggcag cctcgcagga gccgcacagg acgacgatgt ccgccgcagg
     3961 cgcattcggg accatgaccg cgttgcggtc gggcgagggg acggacgctc cgtggtggcg
     4021 gtcggcatcc tcctgccgat ccactggagc gtccagaccg cagcaggagg ggacgttgag
     4081 gccgtccgca gcgcagtccg accccggctt gcagcagggc atcgaggggg cctgacccgt
     4141 aaggcacgag gccgcaggtg ccgcttgcgc cagcaggacc gtcaggagaa ccccgaccca
     4201 cacgaaacgg ctcatgaccg ggcagaatat acgcgggtgg tccgatttca tcaaccggta
     4261 tgaaggttcc gagggggatc ccatga
//
LOCUS       DASRIQ010000552         8093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3614407, whole genome shotgun sequence.
ACCESSION   DASRIQ010000552 DASRIQ010000000
VERSION     DASRIQ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3614407"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /gene="sufB"
                     /locus_tag="VFD06_13665"
     CDS             complement(<1..942)
                     /gene="sufB"
                     /locus_tag="VFD06_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZN04626.1"
                     /translation="MSGSTKTLQDLTTQDYKYGFTTDIEADTVPKGLNEEVIRTISAK
                     KGEPPFMLEWRLKAYRHWLTMTEPTWANVRYPKIDYQDIIYYSAPKSAADGPKSLDDV
                     DPELRRTYEKLGIPLQERAILAGVAVDAVFDSVSVATTFRDKLKSLGIIFGSFSEAVR
                     EHPELVQKYLGSVVPVTDNFFASLNSAVFSDGSFCYVPKGVRCPMELSTYFRINAAKT
                     GQFERTLIIAEEGSYVSYLEGCTAPMRDEHQLHAAVVELIALDNAQIKYSTVQNWYPG
                     DEQGRGGIYNFVTKRGLCKGRHSKISWTQVETGSAITW"
     gene            complement(978..1469)
                     /locus_tag="VFD06_13670"
     CDS             complement(978..1469)
                     /locus_tag="VFD06_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZN04627.1"
                     /translation="MKVSSQEEYGLRCMLQFARRQQEGNDHPLTLGEVARDEGLTVAY
                     VAKLVRRLRRAGLVKSVLGRSGGYTLARQASEISILEILGALGGKLYTADYCRRFSGE
                     RATCMHMGDCSIRSLWGVVEGLLDRVLGATMLKDLLGSERRVGAVLSARNPGIVPVTR
                     ASR"
     gene            1539..2591
                     /gene="hemE"
                     /locus_tag="VFD06_13675"
     CDS             1539..2591
                     /gene="hemE"
                     /locus_tag="VFD06_13675"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZN04628.1"
                     /translation="MPSLQDCALLRACRREPLPSPPVWLMRQAGRYMKEYRALREKVP
                     FLTLCRDPDLVTEVTVFAAERLGVDAAIIFSDLLLPLEPLGFRLAYDRGEGPSVEPPI
                     RSAADLARLRDPDVEADLGYVFEAIRRTRRALPAGMPLLGFAGAPFTLASYLIEGGGS
                     AHYRHTKAFMYADPGAWNALLERLARVTAAFLRGQIAAGAQAVQIFDSWVGCLAPEDY
                     RAFVLPHVKRLVAEVKPHAPVILFGTQTTALLPAIAEAEATVVGVDWRVALLDAWDRI
                     GRGTAIMGNLDPCALFAPHPEVVRRTRALLARAADRPGWIFNLGHGILPHTPVESVEA
                     LVETVHAWNGAGDGSS"
     gene            2561..3553
                     /gene="hemH"
                     /locus_tag="VFD06_13680"
     CDS             2561..3553
                     /gene="hemH"
                     /locus_tag="VFD06_13680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZN04629.1"
                     /translation="MEWRRGRLVVSGALRGDAGSGDPVDSVLLIAFGGPTAPEEIRPF
                     LDHVLRGRPVSPSRCEEVVQHYMEVGGSSPLLPLTRRQAAALEAELARAGTPLPVFVG
                     MRHWHPFLADTLRGMAERGCRRAAGIILAPHPSPVSRQAYEKEVAAGREALGPRAPVV
                     DYCAGWSDHPLFIEALAARLEEARRQLPESRQVSAPVLFTAHSIPVPLSEAGGYEASL
                     RRTAELLARAARVDRWRLVFQSRSGSPDERWLEPDILDALGEESEAGTTEIIVAPIGF
                     VSDHVEVLYDLDVAARRRAAGLRLGFARAGTPGDHPAFVRLLAGLVREMGGNRS"
     gene            3550..5016
                     /gene="hemG"
                     /locus_tag="VFD06_13685"
     CDS             3550..5016
                     /gene="hemG"
                     /locus_tag="VFD06_13685"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZN04630.1"
                     /translation="MSANGTAAPSSEAPPFDVAVVGAGIAGLTAAASLVERSRTRGVP
                     LRLTLFEAGRRPGGVVRTERVDGYLLEGGPDCFITDRPWGLELCRRLGLEKDLVGTNS
                     DCRRSFVLRGRRLLPVPEGFQLLAPARLRTFARSPVLSLAGRARAAMDLVLPRGPALA
                     DESLASFVRRRFGTETLERLAQPLLAGIYGADPERLSLKATMPRFLDLEREHRSVILG
                     LRRGRRRGAASGTSGARYGLFVAPRRGMGAIVERLEQALPAGALRPGAKIAAIEPLSA
                     APAQPRYLVRGAAGDETRVHAVIVALGARDAAALVRPVDASVASLLEAVPYGSSTTVN
                     LGYREEEVPRQLDGFGFVVPRREKRRLVACSFSSVKFPDRAPQGNVLLRAFLVDGAVP
                     AGDDDAAIACARAELEATLRIEAAPGLARAFHWPGAMAQYEVGHLDRVAEIESRLERH
                     VGLALAGNGLRGVGVPDCIRSGEQAAERILSQASLLVS"
     gene            5028..5912
                     /locus_tag="VFD06_13690"
     CDS             5028..5912
                     /locus_tag="VFD06_13690"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HZN04631.1"
                     /translation="MPASSSGRLRLLLALALAALPAIEGTTAHDFTILPETSRPTPEA
                     EFALGMWIGAVFPGEMVAWRTGRVAEFAIVDARGRRDLEAPETDGEPPRARLRLRNPG
                     TAVVALSTDPSYITLEAPEFTAYLEEEGHDAALAARRGAAKTVAQGRERYTRHVKTIL
                     NAGGPGASVALTRVGLALEIVPEKDLSGLRSGASLPVRVFFRNAPYSHGAICASDSAA
                     PAAAHDAGAAPGKGKGPYAWCGRLDGAGRASVPLPRPGWQMLRTTRMIELRDDPKADW
                     HSYWSSLTFEVAASEEGK"
     gene            5909..6355
                     /locus_tag="VFD06_13695"
     CDS             5909..6355
                     /locus_tag="VFD06_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HZN04632.1"
                     /translation="MKLALACDHAGWEMKNALAAFLRKQGHEVQEVFPERFDPQDDYP
                     DAAAAVGRAVAAKTAERGILICGSGVGACVAANKIRGVRAGLCHDTYSAHQGVEHDDV
                     NVLCLGSRVIGEATAQELVTAFLGARFSGEERHARRLRKTMALEGS"
     gene            complement(6395..7351)
                     /locus_tag="VFD06_13700"
     CDS             complement(6395..7351)
                     /locus_tag="VFD06_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZN04633.1"
                     /translation="MALVFDFLNGFHDAANSVATVVSTGTLTPNVAVAWAAFFNFVAA
                     FGFGVKVANTLGKGVVMPEAIDRWVVLGGLAGAIFWNLFTWYFGLPVSSSHALIGGLA
                     GAAVAHAGFGVLLWKGLGRIGMFIVLSPLLGLVLGYVTMRLMILLHATSSPSVVDRRF
                     RRLQLLSAAFYSLGHGTNDAQKTMGIIAVLLFTSGYMKEFHVSTWIILTCHAAIALGT
                     LLGGWRIVKTMGMRITKLKPIGGFSAETAGATMLIGTALWGIPVSTTHTITGAIMGVG
                     ATHRLSAVRWGVATRIIWAWILTIPISGIVAAIAYGLIRLAV"
     gene            complement(7422..>8093)
                     /locus_tag="VFD06_13705"
     CDS             complement(7422..>8093)
                     /locus_tag="VFD06_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZN04634.1"
                     /translation="TRSSPWPRPRAVGAGGTMLSIKKSRRLFFETFEGMARCAVEAAG
                     ILESMFKNGSEAFPAHAARIKDVEHRCDDLVHGAVRELNRVFITPFDREDIHDLVTAL
                     DDVVDLMDAAASRAVLFKVGSDVPDAAALAGVIRRQSEKILDAVGHLNAPQAILDRCK
                     QIKEMETEGDRLYREAMAHLFEKYHTDPIFVIKAKEIIEVLEAGTDAGDRIAIVLERI
                     VLKNQ"
BASE COUNT         1126 a         2849 c         2861 g         1257 t
ORIGIN      
        1 ccaggtgatc gccgaaccgg tctcgacttg cgtccaggag attttcgaat ggcgcccttt
       61 gcagaggccg cgtttggtga cgaaattgta gatgccgccc cgtccctgtt cgtcgccggg
      121 ataccagttt tgcaccgtcg aatacttgat ttgggcgttg tccagcgcga tcaattcgac
      181 gactgccgcg tgcaattgat gctcgtcgcg catcggcgcc gtgcaaccct cgaggtagct
      241 gacgtacgag ccctcctcgg cgatgatcag cgtgcgctcg aactggccgg tcttagcggc
      301 gttgatccgg aagtaggtcg acagctccat ggggcagcgg acgcccttcg gaacgtagca
      361 gaacgatccg tcggagaaga ccgccgagtt caacgacgcg aagaagttgt ccgtgaccgg
      421 cacgaccgaa ccgagatact tctgcacgag ctcggggtgc tcgcggaccg cttccgagaa
      481 cgagccgaag atgatcccca acgacttcag cttgtcgcgg aacgtggtcg ccacggaaac
      541 gctgtcgaac acggcgtcga ccgcgacccc tgcgaggatc gcgcgctcct gcagggggat
      601 gccgagcttc tcgtaggtgc gccggagctc cgggtcgacg tcgtcgagcg acttcggtcc
      661 gtcggccgcc gacttcggcg ccgagtaata gatgatgtcc tggtagtcga tcttcgggta
      721 gcggacgttc gcccaggtcg gctcggtcat cgtcagccag tggcggtacg ccttgagacg
      781 ccactcgagc atgaagggcg gctccccctt cttggcggag atggtccgga tgacctcctc
      841 gttcagcccc ttggggacgg tgtcggcttc gatgtccgtg gtgaagccgt acttgtagtc
      901 ctgggtggtc aggtcctgga gcgtcttggt cgatcccgac attgcttttc tttctccttg
      961 cgtcgtggcc ggagggttca gcgactggcc cgcgtgaccg ggacgatgcc cgggttgcgc
     1021 gcgctgagga ccgccccgac acgacgctcg cttccaagca ggtccttgag catggtggct
     1081 cccaggaccc gatccaggag cccctcgacc accccccata acgagcggat cgagcagtct
     1141 cccatgtgca tgcaggtggc gcgctccccg gagaagcgcc ggcagtagtc ggcggtgtac
     1201 agcttgccgc cgagggcgcc gaggatctcc agaatgctga tctccgacgc ctggcgtgcc
     1261 agcgtgtagc cgcccgaccg gccgaggacg ctcttcacga gcccggcacg ccgcagccgt
     1321 cgcaccagct tggcgacata ggccacggtc aatccttcgt cgcgggcgac ttcccccagc
     1381 gtcagcgggt ggtcgttccc ctcctgctgg cgccgggcga actgcagcat gcagcgcaac
     1441 ccatattctt cctgtgagga aaccttcatg acggcgtcat tttaggaaat gtgtactctt
     1501 ttgtcaacga tccggcatgg taggatttcg cccccgcgat gccgagcctc caggactgtg
     1561 cactccttcg agcctgccgg cgcgagccgc tcccatcccc tccggtctgg ctgatgcgcc
     1621 aggcggggcg ctatatgaag gagtaccggg cgctgcgcga aaaggtcccc ttcctgaccc
     1681 tgtgccgcga cccggatctg gtcaccgaag tgacggtctt cgcagccgag cggctcggcg
     1741 tggacgcggc gatcatcttc tcggacctgc tcctgccgct cgagccgctg gggttccggc
     1801 tggcgtacga ccgtggtgag ggcccttcgg tcgagccgcc gattcgctcc gccgccgacc
     1861 tcgcgcggct gcgcgatccg gacgtcgagg cggaccttgg ctacgttttc gaggcgatcc
     1921 ggcggacccg ccgcgcgctt cccgcgggga tgccgctcct gggattcgcg ggcgcaccgt
     1981 tcacgctcgc ctcctatctc atcgaagggg gcggatcggc ccactaccgc cacaccaagg
     2041 cgttcatgta cgccgacccg ggcgcctgga acgcccttct cgaacggctg gcgcgggtga
     2101 ccgcggcgtt cctgcgcgga cagatcgccg ccggggcaca ggcggtgcag atcttcgatt
     2161 cctgggtggg atgcctcgcg ccggaagact accgggcctt cgtcctgccg cacgtcaaac
     2221 gtctcgtggc ggaggtcaag ccgcacgccc cggtcattct gttcggtacc cagacgacgg
     2281 cgcttcttcc ggcgatcgcc gaggcggagg cgaccgtcgt cggcgtcgac tggcgcgtgg
     2341 ctctcctcga cgcgtgggac cggatcggac gcggcacggc catcatgggg aacctcgatc
     2401 cgtgcgcgct gttcgcgccg caccccgagg tcgtacgccg gacgcgggcg ctcctggcgc
     2461 gcgccgccga ccggcccgga tggatcttca atctcggcca cggcatcctg ccgcacactc
     2521 cggtcgagag cgtggaggcc ctggtggaga cggtgcacgc gtggaatggc gccggggacg
     2581 gctcgtcgtg agcggcgcgc tccgggggga cgccggatcc ggcgacccgg tcgactcggt
     2641 cctcctcatc gcgttcggcg ggcccacggc accggaggag atccggccgt tcctcgatca
     2701 cgtgctgcgc gggcgcccgg tgtcgccctc gagatgcgag gaggtcgtgc agcattacat
     2761 ggaggtcggc ggctcttcgc cgcttctgcc gctgacgcgg cgccaggcgg ccgccctcga
     2821 ggcggagctg gcgcgcgcgg ggactcccct gccggtgttc gtcggcatgc gccactggca
     2881 cccgttcctc gccgacaccc tgagaggaat ggcggagcgc ggctgccgcc gcgccgccgg
     2941 gatcatcctg gcgccgcacc cgtcaccggt gtcgcgtcag gcgtacgaga aggaggtcgc
     3001 ggcggggcgc gaggcgctcg gaccccgcgc tcctgtggtc gactattgcg cgggctggtc
     3061 ggatcacccg ctgttcatcg aagcgctggc ggcgcgcctc gaagaggccc ggcggcagct
     3121 tccggagagc cggcaggtgt cggccccggt gctgttcacg gcgcacagca tccccgtccc
     3181 cctgtccgag gcgggcggct acgaggcgag cctgcggcgc acggccgagc tcctggcgcg
     3241 cgcggcgcgc gtcgatcgat ggcgcctcgt cttccagagc cgcagcggct cccccgatga
     3301 acgctggctc gagccggaca tcctcgatgc ccttggcgag gagtcggagg cggggacgac
     3361 cgagatcatc gtggcgccga tcggcttcgt cagcgaccac gtcgaggtcc tctacgacct
     3421 cgatgtcgcc gcgcggcgtc gcgcggccgg gctgcgcctc gggttcgcgc gcgccggcac
     3481 accgggagac catccggcct tcgtgcggct cctcgccggc ctcgtccggg agatgggagg
     3541 gaaccgatcg tgagcgcgaa cggaacggcc gccccctcgt ccgaagcccc tccgttcgac
     3601 gtcgccgtcg tcggcgccgg catcgcgggc ctgacggcgg ccgcttcgct cgtcgagcga
     3661 tcgaggacac gcggcgtgcc gctccgcctg accctcttcg aggcgggccg gcgaccaggc
     3721 ggcgtggtgc ggaccgagcg ggtcgacggg tacctcctcg aaggagggcc cgactgtttc
     3781 atcaccgatc gcccgtgggg gctcgagctc tgccggcgcc tgggcctcga gaaggacctc
     3841 gtcggcacca actccgactg ccgccgctcg ttcgtgctgc gcgggcggcg gctcctgccg
     3901 gtgccggaag ggttccagct gctcgccccg gcgcgcctgc gcaccttcgc ccgctcgccg
     3961 gtcctgagcc tcgccgggcg ggcgcgcgcc gcgatggatc tcgtcctgcc gcggggaccc
     4021 gcgcttgccg acgagagcct ggcgtcgttc gtgcgccgcc ggttcgggac ggagacgctc
     4081 gagcgcctgg cgcagccgct cctcgcggga atctacggcg ccgatccgga acggctcagc
     4141 ctgaaggcga cgatgccgcg cttcctcgac ctcgagcgcg agcatcgcag cgtcatcctc
     4201 ggcctgcgcc gcggccgccg ccggggcgcc gcctccggca cctccggggc gcgctatggc
     4261 ctcttcgtcg cgccgcggcg cggcatgggg gcgatcgtcg agcgcctcga gcaggcgctc
     4321 cccgccggtg ccctgcgccc gggggcgaaa atcgcggcga tcgagcccct ctccgccgcg
     4381 ccggcgcagc cgcgttacct cgtgcgcggc gccgccgggg acgagacgcg ggtgcacgcc
     4441 gtgatcgtcg cgctcggagc ccgcgacgcg gcggcgctcg tccgcccggt cgacgcgagc
     4501 gtcgcgtccc ttctcgaggc ggtgccgtac ggatcgtcca cgacggtcaa cctcggctac
     4561 cgcgaggagg aggtgccgcg ccagctcgac gggttcggct tcgtggtgcc gcggcgggag
     4621 aagcgacggc tcgtggcctg ctccttctcg agcgtcaagt tcccggatcg ggccccgcag
     4681 gggaacgtgc tcctgcgcgc cttcctcgtc gacggcgcgg tgccggcggg agacgacgac
     4741 gcggcgatcg cctgcgcccg cgccgagctc gaggcgacgc tccggatcga ggccgctccc
     4801 gggctcgccc gcgccttcca ctggccggga gcgatggcgc agtacgaggt cggccacctc
     4861 gatcgggtcg cggagatcga atcgcggctc gagcggcacg tggggctggc gctcgccggc
     4921 aacggtctgc gcggcgtcgg cgtccccgac tgcatccgga gcggcgaaca ggcggccgag
     4981 cggatcctgt cgcaggcctc gctcctcgtg tcatgatcgc cgtcccgatg cccgcgtcct
     5041 cgtccggtcg cctccgcctc ctcctcgcgc tcgcgctcgc cgcgcttccg gcgatcgaag
     5101 gaacgacggc gcacgatttc accatcctcc cggagacgtc ccgccccacc cccgaggcgg
     5161 agttcgcgct cggcatgtgg atcggggcgg tgttccccgg cgagatggtc gcgtggcgca
     5221 cgggccgcgt ggccgagttc gccatcgtgg acgcgcgcgg tcgccgcgac ctcgaagcgc
     5281 cggagaccga cggcgagccg ccgcgcgccc ggctgcggct gcggaacccg gggactgcgg
     5341 tcgtggcgct ttccacggat ccgtcgtaca tcaccctcga ggcccccgag ttcaccgcct
     5401 acctggaaga ggaagggcac gacgcggccc tcgccgcccg ccgcggcgcc gccaagaccg
     5461 tcgctcaggg gcgggagcgc tacacacgtc acgtgaagac catcctcaac gccggcggac
     5521 caggggcctc ggtggcgctg acacgcgtcg gcctggcgct ggagatcgtc ccggagaagg
     5581 acctgtcggg gctgcggtcg ggagcctcgc tgccggtgcg cgtcttcttc cgcaacgccc
     5641 cctactccca cggtgcgatc tgcgcctccg actcggccgc ccccgcagcg gcgcacgacg
     5701 ccggggcggc tccgggcaaa gggaagggtc cctacgcctg gtgcgggcgg ctcgacggcg
     5761 cggggcgcgc ctcggttccg ctgccccggc cgggatggca gatgctccgc accacccgga
     5821 tgatcgaact gcgcgacgat ccgaaggcgg actggcactc gtactggtcg tcgctcacct
     5881 tcgaggtggc ggcgtccgag gaggggaaat gaagctggcg ctcgcgtgcg atcacgccgg
     5941 gtgggagatg aagaacgccc tggccgcctt cctgcgcaag caggggcacg aggtgcagga
     6001 ggtcttcccg gagcggttcg acccgcagga cgactacccg gatgccgccg ccgccgtcgg
     6061 acgggcggtg gcggcgaaga cggcggagcg cggcatcctc atctgcggca gcggcgtcgg
     6121 cgcttgcgtc gccgcgaaca agatccgcgg cgtgcgggcc ggcctgtgcc acgacaccta
     6181 cagcgcccat cagggggtcg agcacgacga cgtcaacgtc ctctgcctcg ggtcgcgcgt
     6241 catcggcgag gcgacggcgc aggaactggt gaccgctttc ctcggcgccc gcttctcggg
     6301 cgaggagcgg cacgcgcggc gcctccggaa gacgatggca ctcgaaggat cctgatcggt
     6361 cggcgccggg tcgcggcggc cgcccggccg actctcaaac cgcgagccgg atgagcccgt
     6421 aggcgatcgc cgcgacgatc ccggagatcg ggatcgtcag gatccaggcc cagatgatcc
     6481 gggtcgcgac cccccagcgg acggcgctca ggcgatgcgt cgcaccgacc cccatgatgg
     6541 cgccggtaat ggtgtgcgtc gtgctgaccg ggatgcccca cagggcggtg ccgatgagca
     6601 tcgtggcgcc ggcggtctcc gccgagaagc cgccgatcgg cttgagcttg gtgatccgca
     6661 tccccatcgt cttgacgatg cgccagccgc cgaggagcgt cccgagcgcg atcgccgcgt
     6721 ggcaggtcag gatgatccag gtcgagacgt ggaactcctt catgtagccg ctggtgaaca
     6781 ggaggaccgc gatgatcccc atcgtcttct gcgcgtcgtt cgtgccgtgg ccgaggctgt
     6841 agaaggcggc cgagaggagc tgcaggcgcc ggaagcggcg gtccacgacc gacggcgaag
     6901 aggtcgcgtg gagcaggatc atcagtcgca tcgtgacgta gccgagcacg aggcccagga
     6961 gcggcgacag cacgatgaac atcccgatgc ggccgagccc cttccacagc aggacgccga
     7021 acccggcgtg cgccaccgct gccccggcca gcccgccgat gagggcgtgc gacgaggaga
     7081 ccggcaggcc gaagtaccag gtgaagaggt tccagaagat ggcgccggcg agcccgccga
     7141 gcaccaccca gcggtcgatc gcctccggca tcaccacccc cttgccgagg gtgttggcga
     7201 ctttgacgcc gaaaccgaag gcggcgacga agttgaagaa cgccgcccag gcgaccgcga
     7261 cgttcggggt cagggtgccg gtcgacacga cggtcgccac cgagttggcg gcgtcgtgga
     7321 agccgttcag gaaatcgaac accagcgcca cgatgatgat cgcgatgacg gacgcaagcg
     7381 tggggtcgat ggcggcctcg ctcccggttc ccgggtgccc gctactggtt cttgaggacg
     7441 atgcgctcga ggacgatggc gatgcggtcg ccggcgtcgg tgccggcctc caacacctcg
     7501 atgatctctt tcgccttgat gacgaagatc gggtccgtgt ggtacttctc gaagagatgc
     7561 gccatcgcct cgcggtacag gcgatccccc tcggtctcca tctccttgat ctgcttgcag
     7621 cgatcgagga tcgcctgcgg cgcgttgagg tgcccgacgg cgtcgaggat cttctccgac
     7681 tgccggcgga tgacgccggc gagggccgcg gcgtcgggca cgtcgctccc gaccttgaac
     7741 agcacggcgc ggctggcggc ggcgtccatc aggtccacga cgtcgtccag cgcggtgacc
     7801 aggtcgtgga tgtcctcgcg atcgaacggc gtgatgaaga cgcggttcag ctcgcgcacc
     7861 gcgccgtgca ccaggtcgtc gcagcggtgc tcgacgtcct tgatgcgcgc cgcgtgggcc
     7921 gggaacgcct cgctgccgtt cttgaacatc gactcgagga tgccggccgc ctcgacggcg
     7981 cagcgcgcca tcccttcgaa ggtctcgaag aacagccggc ggctcttctt gatgctcagc
     8041 atcgtccctc ccgccccgac cgcgcgcggg cgcggccacg gtgacgacct cgt
//
LOCUS       DASRIQ010000553         6092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3615535, whole genome shotgun sequence.
ACCESSION   DASRIQ010000553 DASRIQ010000000
VERSION     DASRIQ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3615535"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VFD06_13710"
     CDS             <1..1077
                     /locus_tag="VFD06_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04635.1"
                     /translation="TLSRTRRDDMSTSVRARRWGIVLAAGLMAAMAPVAEAAPGLGTV
                     KHLRLPTSTDPHDVTTASDGNVWFTVQGAFDPVTFLTPGSVARVTPRGDITEFAVCDF
                     CITNDIVQGPDGILYISDNDGQLRRISTSGEVQSSVPVPPVPPSETGSPLGGIAADST
                     SIWFADSFNNRIGRFDVFTAVFTFYPVALNDGVDDVAVAGDGTVWFIGSEVTETDVIG
                     AIGEIDPGVGVVSMTPLSGVAAGIAVAPNGTVWATEIFADLIARLTPSGSGPHDLVEF
                     PTAVGATPLGIAPAPDGNVWFTQNLRGNIARITQGGVITEAGKVIDFADPKRPDPIGI
                     TIGANGDPWYAESLVDSVATFKLR"
     gene            1210..3906
                     /locus_tag="VFD06_13715"
     CDS             1210..3906
                     /locus_tag="VFD06_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04636.1"
                     /translation="MRRVVQPGKGRSVSGALLLVLLSAVQWSPTARGGDPADAPSASY
                     GEPGVTASVRSIMARPGPERRVAMPIPPAAEHPRRDLPVGRDPEARPVAQWPLPPAPA
                     AAFPGNDPDATAAHPAAPQAIGLGFKAIALADSGAYPPDPSGDVGPEQILVATNGRIR
                     VFDRAGTVGALDTLLETFFDDVRAFQSVSQPHVRYDRLSGRWFVAAITSGFPNRVVIA
                     MSSGSVIDDASSFTFFFFVQDAVPPLGQAGQKVIDLSMGVDKFALYFGGNVVNSSGIF
                     TASSGYLVRKASLLAGGPPSAQAFRGIGTVASGGLWAPRGVDNDDPAATQGYFIGVDI
                     RLTGRLVLRRVTNPGGTPSTLSANINITVPDTALPMSVPTGGSTFPLESWDDRLFAAR
                     VGVNRITGERSLWTAHNIRIDATGTGTTGGDRDGARWYEIRNLGAAAPLVFQAGTLFD
                     EAAEFPRYFFIPSIAMSGQGHAAIGTSTSGENYFADAAVAGRLADDAAGATQPFAQAT
                     FSGTPYDDGFSFPEYWGSKSATVVDPNDAMTMWTVQEWCDDTNSWGVQVVQLMAPPPA
                     TPASASSVCAGLPSASVTVTGTSVSGSGFFDPGPDTGGPGFPNHIQAAIGGGITVDSV
                     AFDSPTQVTLQVSTVGASMGAHDVTITNPDGQSATGTGVLDVVGAPATPAAGADSPVC
                     AGGTLQLTASTISGATYAWTGPNGFTSSQQNPVLIGVDALAAGTYSVTASAAGCSSAE
                     GSVVVSVIDEGGTCAGCGLCEAGACVTGDSDSDGVGNGCDCAPGNPEAWDLPGDASGP
                     TLTHAPPGTGGTTTLVWSAPATGGLAAGMTYDVLRSAAASDFVGAGVCLESDGADLQA
                     ADPEAPAPGAILFYVVRAQNACGAGVSHRDGAGVPRPARDCP"
     gene            3915..>6092
                     /locus_tag="VFD06_13720"
     CDS             3915..>6092
                     /locus_tag="VFD06_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04637.1"
                     /translation="MTPARFVPLVLILGFAPVLARAQCVSAPACALAPCRFSQATSWS
                     SGAGCQAPPDKANEAWLVSPGHAMVLDAARQVTGHGDVQGDLSFDPSPLNRDEHGYRN
                     LIVTTGQGDDLACRTGGSIRLRAGDRLRFDTRGGVGVLAHLNGCVLDFRGSAFPTTAS
                     AVVAADDGPACGNASPVGRQWTITPAAGLEQAKVGRRVIVQSGPLRNRHFEIADVTSG
                     AIVLCSHLADAVSLGERLTPRALVGQPPGPGTIGHHSTPAITAGNESCTGPGSPHTCC
                     RAAGQGTCADGPDPSPGDALLIVDDGTIDDMGGSGWRLVDNRDNSTECNGEPCSGSDP
                     FPIVQYMNLAGFGGGGNNQGVDAASFSVRSADQAVPDFVGNNLHGFKSDYGIVFRGIK
                     NTRIEWNAIHDSAQPAGTSDNYCQLNVHQHKRIEQGRLDTPAANVLIRNNVAWGTKQC
                     AIQVNDAENFDEPHPLFQASGIEVSDNLVYGGCAVGAAAICDGIQVHACRDCQVRRNL
                     CYDMHNSDHSEGSCIKIGGTAGNDGTDVSFNWLVNGSRMGVRCVDDTTAPVNLFNCLH
                     VGITGNFISHFRSSGGRGGRWYGNVVRNFGLTDTVGASGIDNPIRAFGDYVGIDDAVA
                     ATPLCATSPHSCGRAGVAWYRGLGNNGRTDVVASDLVLGRLSANSGGLERHGMDNDRY
                     GTGGVADFNGVIAHVTHDNRAVGPAGAAPTFAFSAGAPPVPVTW"
BASE COUNT          903 a         2242 c         2047 g          900 t
ORIGIN      
        1 actctgtcac gaacaaggag ggatgacatg tcgacatccg tacgcgcgcg ccgatgggga
       61 atcgttcttg ccgccggcct gatggcggcc atggcgccgg tcgccgaggc ggccccagga
      121 ctcggaacgg tcaagcacct caggttgccc accagcacgg atccacacga cgtcacgacc
      181 gcgtcggatg gcaacgtctg gttcacggtc cagggagcgt tcgacccggt gacgtttctc
      241 acccccggct cggtcgcgcg cgtcacgccg cggggcgaca tcaccgagtt cgccgtctgc
      301 gacttctgca tcaccaatga catcgtccaa ggccccgacg ggatcctcta catcagcgac
      361 aacgatggac agttgcgccg gatctccacc tcgggagaag tccaaagcag cgttccggtt
      421 ccaccggttc ccccgagcga gaccgggagc ccgctcggcg gcatcgcggc agactcgacc
      481 agcatctggt tcgcggattc cttcaacaac aggatcggcc gcttcgacgt cttcaccgcg
      541 gtgttcacgt tctacccggt tgctctcaac gacggtgtcg acgacgtcgc ggtagcgggg
      601 gacggaacgg tctggtttat cggatccgaa gtgaccgaaa cggatgtgat cggcgcgatc
      661 ggggagatcg atccaggcgt cggtgtcgtc agcatgacgc cactgagcgg cgtggcggca
      721 gggatcgcgg tcgctcccaa tggcaccgtc tgggcgacgg agatctttgc cgacctcatt
      781 gcgcggctca cgccgagcgg atccggcccc cacgacctcg tcgaattccc gaccgccgtg
      841 ggcgccaccc cgttgggcat cgccccggct ccggatggca acgtgtggtt cacgcagaac
      901 ctccgcggca acatcgcccg gatcacccag ggcggtgtga tcaccgaggc cggcaaagtg
      961 atcgatttcg ccgatcccaa gcgacccgac ccgatcggga tcaccatcgg tgcgaatggc
     1021 gatccctggt acgcggaatc cctcgtggac agcgtcgcca ccttcaagct gcgctgaccg
     1081 agaccggcgg gcgccttcga gacacctgat ccttgacaga tctgcccgat gggcgtactt
     1141 ggagccctga tccacggttc cgggcaggcg gaggtgtgcc ggcccggtgg tggtccgttc
     1201 gggaggggaa tgcgccgcgt cgtccagcct gggaagggcc gatccgtctc tggcgccctc
     1261 ctcctggtcc tcctttcggc ggtccagtgg tcccccacgg cccggggagg cgatccggcc
     1321 gacgcgccgt ccgcctcgta cggcgagccg ggagtcaccg cctcggtgcg ctccatcatg
     1381 gcccgcccgg gcccggagag gagggtcgcg atgccgatcc cccctgcggc cgagcacccc
     1441 cgacgtgacc ttccggtcgg ccgcgatccg gaagcgcgcc cggtcgccca gtggcccctg
     1501 ccgccggcgc cggccgccgc cttcccgggc aacgaccccg acgcgacggc ggcgcatccg
     1561 gcggcgccgc aggcgatcgg cctcggcttc aaggcgatcg cgctggccga ttcgggcgca
     1621 tacccgcccg atccgagcgg cgacgtcggc ccggagcaga tcctcgtcgc gaccaacggc
     1681 cgcatccgcg tgttcgatcg cgccgggacc gtgggggcgc tcgacacgct tctcgagacc
     1741 ttcttcgacg acgtgcgcgc cttccagtcc gtctcgcagc cccacgtccg ttacgaccgg
     1801 ctgagcggac gctggttcgt cgcggcgatc accagcggct tcccgaaccg ggtggtcatc
     1861 gccatgagct ccggcagcgt catcgacgac gcgtcatcgt tcaccttctt cttcttcgtg
     1921 caggacgccg ttcccccgct ggggcaggcc ggccagaagg tcatcgatct gagcatgggg
     1981 gtggacaagt tcgccctcta tttcggcggc aacgtcgtca acagcagcgg gatcttcacg
     2041 gccagcagcg gctacctcgt gcgcaaggcg agcctgctcg cgggagggcc accgagcgcg
     2101 caggccttcc ggggcatcgg caccgtggcc agcggcggcc tctgggcgcc gcgcggcgtg
     2161 gacaacgacg atccggcggc gacgcagggg tacttcatcg gggtcgacat ccgcctgacc
     2221 ggccggctgg tgctgcgacg cgtcaccaac cccggcggca cgccgtccac cctctccgcc
     2281 aacatcaaca tcaccgtccc cgacaccgcg ctgccgatga gcgtcccgac cggcggctcc
     2341 accttcccgc tcgagtcgtg ggacgaccgg ctgttcgccg cccgcgtcgg ggtgaaccgc
     2401 atcaccggcg agcgcagcct gtggacggcc cacaacatcc gcatcgacgc gaccggcacc
     2461 ggcacgaccg gtggggaccg ggacggcgcg cgctggtacg agatccggaa tctcggcgcc
     2521 gccgcccctc tggtgttcca ggccggcacc ctgttcgacg aggccgcgga gtttccccgc
     2581 tacttcttca tcccgtcgat cgccatgtcg ggccagggtc acgcggcgat cggcaccagc
     2641 acctccggcg agaactattt cgccgacgcc gccgtcgccg gccgcctcgc cgatgacgcg
     2701 gccggcgcga cgcagccctt cgcccaggcc acgttcagcg gcacgccgta cgacgacggc
     2761 ttcagcttcc cggagtactg ggggtcgaaa tcggccaccg tggtcgatcc gaacgacgcg
     2821 atgacgatgt ggaccgtcca ggaatggtgc gacgacacca actcctgggg cgtgcaggtg
     2881 gtgcagctga tggcgccgcc ccccgcgacg ccggcatccg ccagcagcgt ctgcgcgggc
     2941 ctgccttccg ccagcgtgac ggtgaccggt acgtcggtct ccggctccgg cttcttcgac
     3001 ccggggcccg acaccggcgg gcccggcttc ccgaaccaca tccaggcggc gatcggcggc
     3061 gggatcaccg tggacagcgt cgccttcgac tcgccgacgc aggtcaccct ccaggtgtcc
     3121 accgtcggcg cctccatggg cgcgcacgac gtgacgatca ccaaccccga cggacagtcg
     3181 gcgaccggga ccggcgtcct cgacgtcgtc ggcgccccgg cgacccccgc cgccggggcg
     3241 gactccccgg tgtgcgccgg cggcacgctg cagctgaccg cgtccacgat ctccggcgcc
     3301 acctacgcct ggaccggacc gaacgggttc acgtcgtcgc agcagaaccc ggtgctgatc
     3361 ggagtggacg ccttggcggc gggaacgtac tcggtcaccg cctcggccgc cggctgctcc
     3421 tccgccgaag gaagcgtggt cgtgtcggtg atcgacgagg gcgggacctg cgccgggtgc
     3481 ggcctctgcg aagcgggcgc ctgcgtgacc ggcgactccg acagcgacgg cgtcggcaac
     3541 ggctgcgact gcgccccggg caatccggag gcgtgggatc tgccgggcga cgcgagcggc
     3601 ccgacgttga cccatgcgcc gccgggcacg ggtgggacca cgaccctcgt ctggtcggca
     3661 ccggccaccg gcggtctcgc cgccgggatg acctacgacg tcctccgcag cgcggccgcc
     3721 tccgacttcg tcggcgcggg cgtctgcctc gaatcggacg gggccgacct gcaggcggcc
     3781 gaccccgaag caccggcgcc cggcgccatc ctcttttatg tggtccgcgc ccagaacgcc
     3841 tgcggcgcgg gtgtgtcgca tcgcgatggc gcgggggttc cccgaccggc acgcgactgc
     3901 ccctgattcc ggccgtgacg cccgcccggt tcgtcccgct cgtcctgatc ctcggtttcg
     3961 ccccggttct cgcacgcgcc cagtgcgtct cggctcctgc ctgcgccctc gccccctgtc
     4021 gcttcagcca ggcgacgagc tggtcgagcg gggccggatg ccaggcgcct ccggacaagg
     4081 ccaacgaggc ctggctcgtg tcgccagggc acgcgatggt cctcgacgcg gcgaggcagg
     4141 tcaccggcca cggcgacgtg cagggggatc tgtcgttcga tccctctccc ctgaaccggg
     4201 acgagcacgg ctatcgcaac ctcatcgtca cgaccggcca gggggacgat ctcgcctgcc
     4261 ggacgggagg atcgattcgg ctgcgggcgg gcgaccggtt gcgcttcgac acgcgcggcg
     4321 gcgtcggggt gctcgcgcac ctcaacgggt gcgtcctcga tttccgcgga tccgccttcc
     4381 cgacgaccgc ctctgcggtc gtcgccgcgg acgacgggcc ggcgtgcggg aacgcctccc
     4441 cggtcggccg gcagtggacc atcacgcccg ccgccggcct cgagcaggcg aaggtcgggc
     4501 gtcgggtgat cgtccagtcg ggcccgctcc ggaaccgtca cttcgagatc gccgacgtca
     4561 cgtccggagc gatcgtcctc tgctcgcacc tcgccgatgc ggtgagcctc ggcgagcgtc
     4621 tgacgccgcg cgccctggtg ggccagccgc ccggcccggg aaccatcggt catcattcca
     4681 ccccggcgat caccgccggc aacgagtcgt gcaccggccc cgggtcgccg cacacctgct
     4741 gtcgcgccgc cgggcagggg acctgcgccg acggccccga tccatctccg ggagatgccc
     4801 tgctcatcgt cgacgacgga acgatcgacg acatgggcgg gtccggctgg cgcctggtgg
     4861 acaaccggga caactcgacc gagtgcaatg gcgagccctg ctccggctcc gatccgtttc
     4921 cgatcgtgca gtacatgaac ctcgcggggt tcggcggagg ggggaacaac cagggggtgg
     4981 acgccgcgtc cttctcggtc cggagcgcgg accaggcggt ccccgatttc gtcggcaaca
     5041 acctgcatgg gttcaagtcg gactacggca tcgtctttcg cggcatcaag aacacgcgga
     5101 tcgagtggaa cgccatccac gactcggcgc agccggccgg gaccagcgac aactattgcc
     5161 agctgaacgt ccaccagcac aagcggatcg agcagggacg gctcgacacg cccgcggcga
     5221 acgtgctcat ccgcaacaac gtcgcatggg ggaccaagca gtgcgccatc caggtcaacg
     5281 atgccgagaa cttcgacgag ccgcatccgc tcttccaggc gagcggcatc gaggtcagcg
     5341 acaacctggt gtacgggggc tgcgccgtcg gcgcggcggc catctgcgat gggatccagg
     5401 tccatgcctg ccgcgactgc caggtgcggc gcaacctctg ttacgacatg cacaattccg
     5461 atcacagcga ggggtcctgc atcaagatcg gcggcaccgc gggcaacgac ggcaccgatg
     5521 tctccttcaa ctggctcgtg aacggcagcc gcatgggcgt ccgctgcgtc gacgacacga
     5581 ccgccccggt gaacctgttc aattgcctgc acgtcgggat caccggcaac ttcatcagcc
     5641 atttccgcag ctcgggcgga cgcggagggc gctggtacgg caacgtcgtc aggaacttcg
     5701 gcctcaccga taccgtcggc gcgagcggga tcgacaatcc gatccgcgcc ttcggcgact
     5761 acgtcggcat cgacgacgcc gtcgccgcca ccccgctgtg cgccacgagc ccccacagct
     5821 gcggccgcgc cggcgtcgct tggtatcgcg gcctggggaa caacggccgg accgatgtcg
     5881 tggcatcgga tctggtcctc ggccggctga gcgccaactc gggcggactg gaacggcacg
     5941 ggatggacaa cgaccgctac ggaaccgggg gtgtggccga cttcaacggg gtgatcgcgc
     6001 acgtcacgca cgacaaccgc gccgtcggcc ccgccggcgc cgctcccacc ttcgccttct
     6061 ccgccggggc gccgccggtc cctgtcacct gg
//
LOCUS       DASRIQ010000554         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3092994, whole genome shotgun sequence.
ACCESSION   DASRIQ010000554 DASRIQ010000000
VERSION     DASRIQ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3092994"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1699)
                     /gene="guaA"
                     /locus_tag="VFD06_13725"
     CDS             complement(128..1699)
                     /gene="guaA"
                     /locus_tag="VFD06_13725"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZN04638.1"
                     /translation="MSGGRGEVGGAGRETIAVLDFGSQFSQLIARRVRELHVFSELLP
                     FDVPLDRLRALKPRGIILSGGPDSVYAPGAPRLDPALFSLGVPVLGLCYGMQLMAHQL
                     GGRVERSPEREYGPADFDILESSPLFADTPKTQRVWMSHGDSILAPPPGFRGIGRTKN
                     APIAAMADDGRRLYALQFHPEVSHTEHGRPILAAFLRAICGCGEGWRLSSFIEESVAA
                     VRDRVGSDGRVIAGLSGGVDSAVAALLIHRAIGTRLQCLFVDNGLLREGEGDEVVRSF
                     RDSFHLPVRRVDAADAFLAALAGVTDPERKRKEIGRVFIEVFDREAKLAGPVEFLAQG
                     TLYPDLIESTSNKGPSAVIKTHHNVGGLPATMRLKLVEPLRYLFKDEVRRVGLELGLD
                     RRFVSRHPFPGPGLAVRVLGEVTRERLDLLRRADAIYIEELRRADLYDATAQAFVVLL
                     PVKSVGVMGDGRTYENVCALRAVTTDDFMTADWARLPHDFLAHVSARIVNEVRGINRV
                     VYDVSSKPPATIEWE"
     gene            complement(1696..2721)
                     /gene="holA"
                     /locus_tag="VFD06_13730"
     CDS             complement(1696..2721)
                     /gene="holA"
                     /locus_tag="VFD06_13730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZN04639.1"
                     /translation="MARLTFPRLRQALLRGQVDPVYLLAGEEAWFHDEGLKLIEAAAL
                     DAAAGINREAFRGSETTLFEIVDLASTYPMGPGRRLVVVRDADALKPEGIEALKAYVA
                     RPNPRTCLIFSDLSFDQRRSLYKTLAAGATAVDCAPLADETAVAAWARDRLQARGYGL
                     SPELAEAIGLGLAGAGLQRLDAELQKLMSAIGSPRPVEAKDLAILADVPRVGDAFQAA
                     RMALRGDRGGAVRSLRALLDAGEEAPMILGAFAWYARTALKARAASERRVAPRDLPAL
                     YALKPGQVERMREEIGPVPEAMLREAVALCLETDRGIKGGGAKDPANAFERMVHRLAR
                     SRARRTR"
BASE COUNT          415 a         1067 c          957 g          369 t
ORIGIN      
        1 atggcgaggg ccggcatccc cagggcgtgc gcctgctcca ccatctcctc cagcttctgc
       61 gccccgtcga gcagcgagaa ctgggagtgg ttgtgcaggt gcacgaagcg gccgtcgtcc
      121 gccatcccta ctcccactcg atggtggccg ggggcttgct gctgacgtcg tacaccaccc
      181 ggttgatgcc gcgaacttcg ttgacgatcc gggcgctgac gtgcgccagg aagtcgtgcg
      241 gcagccgggc ccagtcggcg gtcatgaagt cgtcggtggt cacggcgcgc agcgcgcaga
      301 cgttctcgta ggtccggccg tcccccatca ccccgaccga cttcaccggc aggaggacca
      361 cgaacgcctg cgccgtggcg tcgtacaggt cggcccggcg cagctcctcg atgtagatcg
      421 cgtcggcgcg gcgcaggagg tccaggcgct cccgcgtcac ctcgccgagc acccgcaccg
      481 ccagccccgg tccggggaac gggtggcggg agacgaagcg gcgatcgagc cccagctcga
      541 ggccgacccg gcgcacctcg tccttgaaca ggtagcggag cggctcgacg agcttgaggc
      601 gcatcgtcgc cggcagcccg ccgacgttgt ggtgcgtctt gatcaccgcc gacggcccct
      661 tgttcgaggt ggactcgatg aggtccgggt acagcgtccc ctgggcgagg aactccaccg
      721 gtcccgccaa cttcgcctcg cgatcgaaga cctcgatgaa gacgcgaccg atctccttgc
      781 gcttccgctc cgggtcggtc acgcccgcga gagcggcgag gaacgcatcg gcggcgtcga
      841 cccggcggac cggaaggtgg aagctgtcgc ggaaggagcg caccacctcg tccccctccc
      901 cttcccgtag gagcccgttg tcgacgaaca ggcattgcag gcgcgtcccg atcgcccggt
      961 ggatgaggag ggccgcgacg gccgaatcga caccacccga cagcccggcg atcacccggc
     1021 cgtcgctccc gacccggtcg cggaccgccg cgaccgactc ctcgatgaac gacgacagac
     1081 gccacccctc gccgcagccg cagatcgccc gcaggaaggc cgcgaggatc ggccgcccgt
     1141 gctccgtgtg cgacacctcc gggtggaact gcagcgcgta caggcgccgc ccgtcgtccg
     1201 ccatggcggc gatcggggcg ttcttcgtgc ggccgatgcc ccggaatccc gggggcggcg
     1261 ccaggatcga atcgccgtgg ctcatccaca cccgctgcgt cttcggcgta tcggcgaaca
     1321 gcggcgacga ctccaggatg tcgaagtcgg cgggaccgta ctcccgctcg ggggagcgct
     1381 cgacccggcc gccgagctgg tgcgccatca gctgcatccc gtagcacagc ccgaggaccg
     1441 gcacgccgag ggagaacagc gccgggtcga ggcgcggggc tcccggcgcg tacaccgagt
     1501 cgggcccgcc cgaaagaatg atgccgcgcg gtttcagcgc ccgcagtcga tcgagcggca
     1561 cgtcgaacgg cagcagctcc gagaagacgt gcagctcgcg cacccgccgc gcgatcagct
     1621 ggctgaactg cgagccgaaa tcgaggacgg cgatcgtctc gcgtcccgcc cccccgacct
     1681 ccccccgacc tccgctcacc gcgtcctcct ggcgcggctc cgcgccagcc ggtgcaccat
     1741 ccgctcgaac gcgttcgccg gatccttcgc gccgcccccc ttgatcccgc gatcggtctc
     1801 gaggcagagc gccaccgcct cgcgcagcat cgcctcgggc accgggccga tctcctcccg
     1861 catccgctcc acctgtcccg gcttgagggc gtacagcgcc ggaaggtccc ggggcgcgac
     1921 ccgcctttcc gaggcggcgc gcgccttgag cgcggtgcgc gcgtaccagg cgaacgcccc
     1981 caggatcatc ggcgcctcct cccccgcgtc gaggagcgcc cggaggctcc ggacggcccc
     2041 gccccgatcg ccgcggagcg ccatgcgcgc cgcctggaag gcgtcgccga cccgcggcac
     2101 gtccgccagg atggcgaggt ccttcgcctc caccggccgg ggcgagccga tggcgctcat
     2161 cagcttctgc agctcggcgt cgagacgctg caggcccgcg cccgcgagcc cgagcccgat
     2221 cgcctcggcc agctcgggcg acaggccgta gccgcgcgcc tgcagccgat cgcgcgccca
     2281 ggcggccacc gccgtctcgt cggcgagtgg cgcgcagtcg acggcggtcg ccccggccgc
     2341 aagcgtcttg tagagcgacc ggcgctgatc gaacgacagg tcggaaaaga tcaggcacgt
     2401 gcgcgggttc gggcgcgcca catacgcctt gagcgcctcg atcccctccg gcttcagcgc
     2461 atccgcgtcg cgcaccacga ccagccgccg accgggcccc atcgggtagg tcgaggcgag
     2521 atcgacgatc tcgaagaggg tcgtctcgga gccgcggaac gcctcgcggt tgatccccgc
     2581 cgccgcgtcg agcgcggccg cctcgatgag cttcagcccc tcgtcgtgga accaggcctc
     2641 ctcgcccgcg agcaggtaga ccggatcgac ctgtccgcgg aggagcgcct gccggagccg
     2701 ggggaaggtc aggcgcgcca ctcagaatcc ttccaggatg gaggtcacga cgctgcgggc
     2761 gaagtcggtg gccacgtcgt cgatcgcgac gatctcctga tcgacgaa
//
LOCUS       DASRIQ010000555         4160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3893749, whole genome shotgun sequence.
ACCESSION   DASRIQ010000555 DASRIQ010000000
VERSION     DASRIQ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3893749"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFD06_13735"
     CDS             complement(<1..289)
                     /locus_tag="VFD06_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZN04640.1"
                     /translation="MTQQGSVTRIAVIPGDGIGVEVTREAVETLRAVTAAGGRPVVFE
                     DFPYGADHYLRTGETLPQSGYERLQTFDAILLGALGDPRVPDNRHAADILLG"
     gene            complement(381..770)
                     /locus_tag="VFD06_13740"
     CDS             complement(381..770)
                     /locus_tag="VFD06_13740"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZN04641.1"
                     /translation="MRPTFARRVSTWGPAVVYLSLIYYVSSQPQVGWATPYPDKLLHV
                     SEYLVLAVLLARALNNGIRRPVSSAMLLLTWGLCVAYAVTDEIHQTFVPGRSPDPRDA
                     LADACGAALGLLALQAAQGAWLRRAPR"
     gene            complement(767..949)
                     /locus_tag="VFD06_13745"
     CDS             complement(767..949)
                     /locus_tag="VFD06_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04642.1"
                     /translation="MIPSHVLNMAAFAVLVAIFFAFLTQNRAKDRARVIAVLGGAMIV
                     LSLIVAYVMYPFPLAR"
     gene            complement(953..1432)
                     /locus_tag="VFD06_13750"
     CDS             complement(953..1432)
                     /locus_tag="VFD06_13750"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HZN04643.1"
                     /translation="MDDPVQEPSPQPPARPRVSPFLPCFLAWCVPGLGHLSLGRTARA
                     VAFFLVVTATFGLGLASGGTASVVDPQQPLSYLATFDNLALGPLDLVGRSVSLGGLVY
                     RLPEEESSPERQAVMNRMRDRVRMPTYEYGSTFLLTAGLMNILLILDAFDIARGRKD"
     gene            complement(1444..1887)
                     /gene="rplI"
                     /locus_tag="VFD06_13755"
     CDS             complement(1444..1887)
                     /gene="rplI"
                     /locus_tag="VFD06_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZN04644.1"
                     /translation="MRVVLRENIDKLGRRGEVREVAPGYARNFLLPKGKALPATDGNM
                     KRIEQEGRRYAVIQAKEKEDAAAVGRRIAGISCTIVRKVGENDQLYGSVTASDIAEFL
                     EKEGIAIDKRRILLEEPIKALGIYTIPVKLHADVQGEVRVWVVKE"
     gene            complement(1912..2193)
                     /gene="rpsR"
                     /locus_tag="VFD06_13760"
     CDS             complement(1912..2193)
                     /gene="rpsR"
                     /locus_tag="VFD06_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZN04645.1"
                     /translation="MGRRDGGSSRGDAKGRKPFKRKFLFRKKKFCKFCEEKLHFIDYK
                     DVRLLQGFVPERSKIFPRRISGTCSKHQRKVMRAIKRARALALIPFTSD"
     gene            complement(2210..2581)
                     /gene="rpsF"
                     /locus_tag="VFD06_13765"
     CDS             complement(2210..2581)
                     /gene="rpsF"
                     /locus_tag="VFD06_13765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZN04646.1"
                     /translation="MLQYETIFMTDPNLTDEEIDAITALVESITTSAGGKVLKTEKWG
                     KRRLAYPVEHREEGIYTLMLLECPPAQVKEIDRRYRMNDRILRHLTVRMEDESQLGPS
                     PMMRPRPAERDEIPPDAGIAP"
     gene            complement(2594..3172)
                     /gene="pth"
                     /locus_tag="VFD06_13770"
     CDS             complement(2594..3172)
                     /gene="pth"
                     /locus_tag="VFD06_13770"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZN04647.1"
                     /translation="MVVGLGNPGRRYQGTRHNAGFMVVEELRRRAGHPEESERAGAMR
                     CTVSLGEQTVTLLRPVEYMNRSGGAVRAAMSRSGIDPSEVLVVSDDLWLPFGTLRVRR
                     SGSHGGHNGLRSIFESLGTNEVPRLRVGIGPAPDEADQADFVLEPFTRAERGRLDEVV
                     TEAADCVETIARDGLTAAMNRYNRAAGAASEE"
     gene            complement(3197..3910)
                     /locus_tag="VFD06_13775"
     CDS             complement(3197..3910)
                     /locus_tag="VFD06_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZN04648.1"
                     /translation="MKELVVDVTRREVLGKNASRRLRRSGSIPAVLYGGGKEPVILQV
                     DPVRIEQILRSESGVNTIFQLNLAGTDQRRHVMIREYQVDPLEGELLHADFLRIAMDA
                     VIEVEVPIRLHGESAGVKLDGGILEPVTRTVRVSCLPGDIPEHLDLDVSAMKINDHLS
                     VADLPKSDKFTLLSDPGLIVVVVAPPAKEEAAATPSVEAAAPAPSEPEVIKKGKQVAE
                     GEEGKADEADKKGKPEAKK"
     gene            complement(3963..>4160)
                     /locus_tag="VFD06_13780"
     CDS             complement(3963..>4160)
                     /locus_tag="VFD06_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HZN04649.1"
                     /translation="HAVLSGPAVDRIRKMEMAQTVVTNSIPLDPARAKAGRVTVLSVA
                     GLLSEAIRRIHTNSSVSSLFV"
BASE COUNT          619 a         1490 c         1384 g          667 t
ORIGIN      
        1 tgccgagcag gatgtcggcg gcgtgccggt tgtccggcac ccgcggatcg ccgagcgccc
       61 cgagcaggat ggcgtcgaac gtctgcaggc gctcgtatcc ggactgaggc aatgtctcgc
      121 cggtgcgcag gtagtggtcg gcgccgtacg gaaagtcctc gaagacgacc gggcggcccc
      181 cggcggcggt gaccgcccgc agggtctcca ccgcctcgcg cgtcacctcc acgccgatgc
      241 cgtcccccgg aatcaccgcg atgcgcgtca ccgatccctg ctgggtcacg aaaccctcct
      301 cccggtcctt cggtggaatc cgccggcccg gcgcgccggc atgttaggcc ctcgactctc
      361 cggccgcaac cgcggatccg tcagcgcggc gcccgccgga gccacgcccc ctgcgccgcc
      421 tggagcgcca ggagcccgag cgccgccccg caggcgtcgg ccagggcgtc gcgcggatcg
      481 gggctgcgcc cgggcacgaa cgtctggtgg atctcgtcgg tcaccgcgta cgcgacgcac
      541 agtccccacg tgagcaggag catcgccgaa ctcacgggac ggcggatgcc gttgttgagc
      601 gcgcgcgcca ggagcaccgc caggaccagg tactccgaga cgtgcagcag cttgtccggg
      661 tacggcgtcg cccatccgac ctggggctgg gacgacacgt aatagatgag ggagaggtac
      721 accaccgccg gtccccaggt ggagacccgg cgcgcgaaag tcggtctcac cgggcgagcg
      781 ggaaggggta catcacgtag gcgacgatga gcgacaggac gatcatcgcc cccccgagca
      841 ccgcgatgac gcgcgcccga tccttcgccc ggttctgcgt caggaacgcg aagaagatcg
      901 ccacgaggac ggcgaacgcc gccatgttga ggacgtggct cggaatcaag ggtcagtcct
      961 tgcgcccgcg ggcgatgtcg aaggcgtcga ggatgagcag gatgttcatc agccccgcgg
     1021 tcaggaggaa cgtgctgccg tactcgtagg tcggcatccg gacccggtcg cgcatgcggt
     1081 tcatcaccgc ctggcgctcc gggctcgact cctcctccgg caggcgatag acgagcccgc
     1141 cgagcgacac cgaccggccg acgagatcga gcggcccgag ggcgaggttg tcgaaggtgg
     1201 cgaggtacga caacggctgc tgcggatcga ccaccgacgc ggtcccgccg gaggcgaggc
     1261 cgagaccgaa agtcgcggtc acgacgagga agaacgccac ggcacgggcg gtccggccga
     1321 ggctcaggtg gccgagcccc ggcacgcacc aggccaggaa acagggcagg aacgggctga
     1381 cgcgcggacg cgccggcggc tgcggggagg gttcctggac cggatcgtcc acctaggaga
     1441 tcctcactcc ttcacgaccc agacgcggac ctctccctgc acgtcggcgt gcagcttcac
     1501 cgggatggtg tagatcccga gcgccttgat cggctcctcc aggaggatac gccgcttgtc
     1561 gatggcgatc ccctccttct cgaggaactc cgcgatgtcg gaggcggtca ccgatccgta
     1621 cagctgatcg ttctcgccga ccttgcggac gatggtgcag gagatcccgg cgatgcggcg
     1681 gccgacggcg gcggcgtcct ccttctcctt cgcctggatc acggcatagc ggcggccctc
     1741 ctgctcgatg cgcttcatgt tgccgtcggt ggccggcagc gccttcccct tcggcagcag
     1801 gaagttgcgg gcgtagccgg gggccacctc gcgcacctcg ccacggcgcc cgagcttgtc
     1861 gatgttctca cgcagaacga cgcgcatcac attcctccgg tcggcggatc tctagtcgga
     1921 cgtgaagggg atcagcgcca gcgcccgcgc ccgcttgatg gcgcgcatca ccttgcgctg
     1981 gtgcttggag caggtcccgg agatgcgccg cgggaagatc ttcgagcgct ccgggacgaa
     2041 cccctgcagc agccgcacgt ccttgtagtc gatgaagtgc agcttctcct cgcagaactt
     2101 gcagaacttc ttcttgcgga acaggaactt ccgcttgaac ggcttgcgac ctttcgcgtc
     2161 gccgcgcgaa ctgcctccgt ctctgcgccc catggtgctc ctgccttcct taaggcgcga
     2221 tgcccgcgtc gggcgggatc tcgtcgcgct cggcggggcg cggccgcatc atcggcgacg
     2281 gccccagttg cgattcgtcc tccatgcgca ccgtgaggtg ccggaggata cggtcgttca
     2341 tgcggtagcg gcggtcgatc tccttcacct gcgccggcgg gcactcgagg agcatgaggg
     2401 tgtagatccc ctcctcgcgg tgctccacgg gataggcgag tcggcgcttc ccccacttct
     2461 cggtcttgag gaccttgccc cccgccgagg tggtgatcga ctcgacgagc gccgtgatcg
     2521 cgtcgatctc ctcgtcggtg agattcgggt cggtcatgaa aattgtttcg tattgtagca
     2581 tcgtgcgttt ccctcactcc tctgacgcgg cgccggccgc gcgattgtaa cggttcatgg
     2641 ccgccgtcag gccgtcgcgc gcgatcgtct cgacgcagtc ggccgcctct gtcaccacct
     2701 cgtcgagccg gccgcgctcc gcgcgcgtga acggctcgag gacgaaatcg gcctggtccg
     2761 cctcgtccgg tgccggcccg atgccgaccc ggaggcgcgg cacctcgttc gtcccgagac
     2821 tctcgaagat cgagcgcagt ccgttgtggc cgccgtggct gcccgaccgc cgcacccgca
     2881 gcgtgccgaa cggcagccag agatcgtccg agacgaccag gacctcggac gggtcgatgc
     2941 cggaccgcga catcgccgcc cgcaccgccc cgccgctgcg gttcatgtac tcgaccggac
     3001 gcagaagcgt caccgtctgc tctcccaggg agaccgtgca ccgcatggcc cccgccctct
     3061 ccgactcctc cgggtgtccg gcccggcgcc gcagctcctc caccaccatg aacccggcgt
     3121 tgtgccgcgt cccctggtag cgccggcccg ggttgccgag accgaccacc atccggagca
     3181 cggccggaac ctccggctac ttcttcgcct ccggcttgcc cttcttgtcc gcctcgtccg
     3241 ccttgccttc ctccccttcc gccacctgct tccccttctt gatgacttcg ggctcggacg
     3301 gagcgggcgc ggccgcctcc acgctggggg tcgccgcggc ctcctcctta gccggcgggg
     3361 cgacgacgac cacgatgagc cccggatcgc tgaggagggt gaacttgtcg ctcttcggca
     3421 ggtccgccac gctcaggtgg tcgttgatct tcatcgccga cacgtcgaga tcgagatgct
     3481 ccggaatgtc ccccggcagg caggagacgc gcacggtgcg cgtgaccggc tcgaggatgc
     3541 cgccgtccag cttgacgccg gccgactccc cgtgcagccg gatcggcacc tcgacctcga
     3601 tcaccgcgtc catggcgatg cgcaggaagt cggcgtgcag cagctcccct tcgaggggat
     3661 ccacctggta ctcgcggatc atcacgtgcc gacgctggtc ggtgccggcc aggttgagct
     3721 ggaagatcgt gttgaccccc gactccgagc gcaggatctg ctcgatgcgg acgggatcca
     3781 cctggaggat caccggctcc ttgccgccgc catacagcac cgccgggatc gagccggacc
     3841 ggcgaaggcg gcgggaggcg ttcttcccga gcacctcgcg tcgcgtcacg tccaccacca
     3901 actctttcat cggtcctgcc ctcctgcggg tccgcgaccc ttttccttca aggtaccggc
     3961 cgtcagacga acagcgagct gacggacgag ttcgtgtgaa tgcgccggat ggcctcgctc
     4021 aggagcccgg ccaccgacag caccgtcacg cggccggcct tggcccgtgc cgggtcgagc
     4081 gggatgctgt tggtgacgac ggtctgcgcc atctccatct tccggatgcg atcgaccgcg
     4141 ggtcccgaaa ggacggcgtg
//
LOCUS       DASRIQ010000556         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3894124, whole genome shotgun sequence.
ACCESSION   DASRIQ010000556 DASRIQ010000000
VERSION     DASRIQ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3894124"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2326)
                     /locus_tag="VFD06_13785"
     CDS             complement(<1..>2326)
                     /locus_tag="VFD06_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN04650.1"
                     /translation="NTPAKVEALFDVAESPIVAKVNQALDARGFPYTVEFQRLGLNLN
                     IQLADGYAGGGINVDNDVDDDGGNSLLGQRMDQYYSRTLGGWPQGLFRFNADDFPGDG
                     VRPTTVLPSQRTFGPFQNPDNSPGFNGDESGYTGYVLPNPIPTAPPELLPYPLPGAVL
                     AGTCTGGALAGDPCAVGNASDPCVVQGGACTPQANTIAGPVRNFDATLIGYEGGFARV
                     NSLDTVENVMNFAPGSAGNRWQIGIGFWAIESSSLQTDYGIGFDDVVFEWNEWHPEDE
                     AALGAPPACSRFAGAGQPAGGQCATVTVDRTTLYECDESIEVAVHDAKCLALGAGAAT
                     TLGGACLADSDCGSGGVCTAARPSVEVAVVGDSDANAVVVDGETLLWPTAKRFTLPAV
                     PGSPGLYKGRVPVTTIENDAAHLYVNPATDRRFAVYYFDPLCDGDRDGQAGEDGFENT
                     DGDGIPDASDKCPLVHDPGQADADGDGRGDLCDNCPGVANANQADTDADGAGDACEFE
                     DVDADGFDNAEDNCPDVRNPNQSDVDGNGRGDLCDTLKTSGVTFGGSNGAADCAFAGL
                     GSKCARPGAALGVACVSDDEFCIRSCNAGVCTQGDNGSAGGSSCVGVANVIQACTSDA
                     NCTLCSGGANHGLPCIRNLAGECPRGCIGGTNPGAVCSTNANCTGGGVCSQIGVCTVG
                     VCFNNFVSPTPTIGQTCVTDADCYVDIDRDADGVLDLLDNCVLTANGPLAGPGNQVDS
                     DADGLGDACDGDCAGTVVVGRCRANGAICPNPGTS"
BASE COUNT          314 a          809 c          832 g          371 t
ORIGIN      
        1 gctcgtcccg ggattcgggc agatggcgcc gttggcgcgg cagcgcccca cgaccaccgt
       61 cccggcgcaa tcgccgtcgc acgcatctcc gagtccgtcg gcgtcggaat cgacctggtt
      121 gcccggcccg gccagcggac cgttcgccgt caggacgcag ttgtcgagga ggtcgaggac
      181 cccgtcggcg tcacggtcga tatcgacgta gcagtcggcg tccgtcacgc aggtctgacc
      241 gattgtcggt gtcggggaga cgaagttgtt gaagcagacg cccacagtgc agacgccgat
      301 ctggctgcac acgccgccgc cggtgcagtt cgcgttggtg gagcagaccg ccccaggatt
      361 cgtgcctccg atgcaacctc tggggcactc ccccgcgagg ttcctgatgc acgggaggcc
      421 atggttcgcg ccgccggagc agagggtgca gttcgcatcg ctggtgcagg cctggatgac
      481 gttcgccacg ccgacgcagc tggacccgcc cgccgagccg ttgtcgcctt gcgtgcacac
      541 gccggcgttg cacgaccgga tgcagaactc gtcgtcgctg acgcaggcga cgccgagtgc
      601 ggcgccgggc cgggcgcact tgcttcccag gccggcgaaa gcgcaatcgg ccgctccgtt
      661 gcttcccccg aacgtcacgc ccgatgtctt gagcgtgtcg cacaggtcgc cgcggccgtt
      721 cccgtcgacg tcggactgat tcggattgcg gacatccgga cagttgtcct ccgcgttgtc
      781 gaagccgtcg gcgtcgacgt cctcgaactc gcaggcatcc cccgcgccgt cggcgtcggt
      841 gtcggcctgg ttggcgttgg cgacgccggg acagttgtcg cacaggtcgc cacgcccgtc
      901 gccgtcggcg tccgcctggc ccgggtcgtg gacgagcggg cacttgtcgg acgcgtccgg
      961 gatgccgtcc ccgtcggtgt tctcgaagcc gtcctccccc gcctgcccgt cgcgatcgcc
     1021 gtcgcacagc ggatcgaagt agtagacggc gaagcgccga tcggtcgcgg ggttgacata
     1081 caggtgcgcc gcgtcgttct cgatcgtcgt taccggcacg cgccccttgt agaggccggg
     1141 tgacccgggc acggccggaa gagtgaaacg cttggcggtc ggccacagca gcgtctcgcc
     1201 gtccacgacg accgcgttcg cgtcgctgtc gccgaccacc gccacctcga ccgacggacg
     1261 cgcggcggtg cagacaccac cggatccgca gtcggagtcg gcgaggcagg cgccgccgag
     1321 cgtcgtcgcc gcgccggcgc cgagagcgag gcacttcgcg tcgtgcacgg cgacctcgat
     1381 cgactcgtcg cactcgtaga gggtcgtgcg gtcgaccgtg acggtggcgc actgcccgcc
     1441 cgccggctgg ccggcccccg cgaagcggga acaggccggt ggtgcgccga gggccgcctc
     1501 gtcttccggg tgccactcgt tccactcgaa gaccacgtcg tcgaacccga tgccgtaatc
     1561 cgtctggagg ctgctgcttt caatcgccca gaagccgatg ccgatctgcc agcggttccc
     1621 cgccgacccg ggcgcgaagt tcatgacgtt ctcgacggtg tcgaggctgt tgacgcgcgc
     1681 gaaccccccc tcgtagccga tcagggtcgc gtcgaagttg cgcaccggcc ccgcgatggt
     1741 gttggcctgc ggcgtgcacg cgcccccctg gacgacgcag ggatccgagg cgttgccgac
     1801 ggcgcacggg tcgccggcca gggcgcctcc cgtgcaggtg cccgccagga cggcgcccgg
     1861 cagcgggtac ggcagcagct ccggtggggc ggtcgggatc ggattgggca gcacgtagcc
     1921 ggtgtagccg ctctcgtcgc cgttgaagcc gggggagttg tccgggttct ggaacgggcc
     1981 gaacgtccgc tgggacggca ggaccgtcgt cgggcggacc ccgtccccgg ggaagtcgtc
     2041 ggcgttgaag cggaagagcc cctgcggcca cccgcccagc gtgcgcgagt agtactggtc
     2101 catgcgctgg ccgaggaggc tgttgccgcc gtcgtcgtcc acgtcgttgt cgacgttgat
     2161 gccgccgccc gcatagccgt cggcgagctg gatgttgagg ttcagcccga ggcgctggaa
     2221 ctcgacggtg tacgggaagc ctcgcgcgtc gagcgcctgg ttgaccttgg cgacgattgg
     2281 cgactccgcg acatcgaaga gcgcctccac cttggcgggg gtgttg
//
LOCUS       DASRIQ010000557         5770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3617103, whole genome shotgun sequence.
ACCESSION   DASRIQ010000557 DASRIQ010000000
VERSION     DASRIQ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3617103"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFD06_13790"
     CDS             <1..219
                     /locus_tag="VFD06_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HZN04651.1"
                     /translation="KCNGTGYKGRVGLYEVMEISDSIREMILTGASSVELKNKAIEEG
                     MITLRGSGLRKIQAALTTVEEVVRETVL"
     gene            240..1364
                     /locus_tag="VFD06_13795"
     CDS             240..1364
                     /locus_tag="VFD06_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZN04652.1"
                     /translation="MAVTLHQLLKTLVEQGGTDLHVTTNSPPQIRVDGKLVPLQLPPL
                     TAPETKALAYSVLTDQQKHRFEENLELDFSFGVRGLARFRANIFSQRGAVAAAFRTIP
                     WEVKSFQDLGLPDVIANLCEKPRGLVLVTGPTGSGKSTTLAAMIDKINNEDHGHIVTI
                     EDPVEYLHGHKSCVVNQRELFADTHSFSNALKSVLRQDPDTVLIGEMRDLETIEAALR
                     IAETGHLTFATLHTNSAAQTINRIIDVFPAHQQSQIRAQLSFVLEGILCQTLLPKASG
                     KGRALAIEVMIPNSAIRNLIREDKVHQIYSMMQTGQSQFGMQTFNQSLSTLYFKKQIS
                     LTTALSHSSNPDELQEMINRGAGLINQPTGSGTAARMAKK"
     gene            1380..2582
                     /locus_tag="VFD06_13800"
     CDS             1380..2582
                     /locus_tag="VFD06_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN04653.1"
                     /translation="MPVYAWKGKNRMGKTQEGTLTADSKDAVIALLRRQQIMVAAVTE
                     KGKEIAIPKFGGGVKQKEIAIFTRQFSVMIDAGLPLVQCLEILGGQQKNRVFQKVLFQ
                     VRQDVESGMTLADSLRKHPKVFNDLFCNMIAAGESGGILDTILQRLSTYIEKRVKLQS
                     AVRSAMIYPTAVITIAIGVVVVILWKVIPTFAMLFQGLGATLPLPTRITIAASRFLGR
                     FMPVLVIGFAAAAFFLHRYYQTYKGRRQIDGFVLKMPVMGDLMRKIAVARFCRTLGTL
                     IQSGVPILEGLEITARTAGNSIVEDAIMATRKSIEEGKTIAGPLEETTVFPSMVVQMI
                     SVGEQTGALDTMLSKIADFYEDEVDEAVANLMSLLEPILILFLGVMIGGIVISMYMPM
                     FSLIGKIA"
     gene            2592..4262
                     /locus_tag="VFD06_13805"
     CDS             2592..4262
                     /locus_tag="VFD06_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN04654.1"
                     /translation="MPAPSSEAGRQLQWLMTFRVVITTTLLVCTFVIELLYRPMLPLL
                     PFYVLAGSAYLLTLVYAVALRTRRALRLQAFLQLAGDATIVTGFVYLTGGAQSPFSFL
                     YLPGIVAGCILLLRRGGFAVALTSWFLYAGLVLVLQRGLLPPSPQALFQEPSVPPVRI
                     VYALLAHLVSFVALAYLASHLTESLRRTGAELERQHGDLLELRALHDNIIDSINSGLL
                     TTDASGRITFANRAAAEITGRPPAGLVAADIVALLRQPERFLQEIRAALDRRSRHRFE
                     IDWKDAMGRDLFLGFTVSVLRERDGRPVGMIFIFQDLTEIRVLEEEVALKKRMAALGE
                     MAAGVAHELRNPLASLSGSVQVLQRDLRPRGEAGELMEIVVKETKRLDGIIRDFLLFA
                     KPGRFHPREVDVAPLLADTLSLLEHSDERRPDHRIVTEAGSDPVPAYVDVNRMKQVFW
                     NLAKNALKAMPQGGTLVARARRGPEGAQVTFTDSGVGMSEEEVAKSFQPFHTTFVGGS
                     GLGLSIVYRIVQEHGGRIQVQSRKGAGTTITLALPARAPAREVAWIAS"
     gene            4247..5647
                     /locus_tag="VFD06_13810"
     CDS             4247..5647
                     /locus_tag="VFD06_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN04655.1"
                     /translation="MDRILIVDDEAGMRDMLSILLRKEGYGVAVADRAERAIEMVSKG
                     EFDLVMSDISMPGKSGLEVLRQARVASPDTPVILITAYASTESAVEALKLGAYDYLIK
                     PFDVEELKNVVRNALEKRRLETENRVLKRELKQKTGPDTLVGESPRMREVLALVDRIA
                     PTHSTVLIVGESGTGKELAARAIHASSPRRDRPFVSINCGALPDELLESELFGHTRGS
                     FTGAVTSKKGLFEVADGGTIFLDEIGDTSPAMQVKLLRVLQERRIRRVGGTDETEVNV
                     RVLAATNHDLEALVREKRFREDLYYRINVIQIQMPALREKREDIPRLVDHFVSKYSAL
                     MGKKVSGVNEEARRSLQQHEWPGNVRELENVIERAVALANGDVLGPADLSRDLGGAAR
                     AAKDLPAELTDAGIDLERQLEGLREHFMSLALDRASGVQTRAAELLGMSFRSFRYFAK
                     KYHLLDGRREPRAGAA"
BASE COUNT          970 a         1951 c         1957 g          892 t
ORIGIN      
        1 aagtgcaacg gcaccggcta caagggccgc gtcggcctgt acgaggtgat ggaaatttcc
       61 gattcgatcc gcgaaatgat cctgacgggg gcgtcgtccg tcgagctgaa gaacaaggcc
      121 attgaagagg gaatgatcac gctccgcggc tcggggctgc gcaagatcca ggcggccctg
      181 acgaccgtgg aagaggtcgt gcgcgagacg gttctctaag agatccaggg gagggactca
      241 tggcggtgac gcttcaccaa ctgttgaaaa cgctggtcga gcaggggggc acggacctgc
      301 acgtcacgac caattcgccc ccccagatcc gcgtggatgg caagctggtg cccctgcagc
      361 tcccgccgct cacggcgccg gagaccaagg cgctggccta cagcgtcctc accgaccagc
      421 agaagcaccg gttcgaggag aacctcgagc tggacttctc gttcggcgtg cgggggctgg
      481 cgcggttccg cgccaacatc ttcagccagc ggggtgctgt ggcggcggcg ttccggacca
      541 tcccatggga ggtcaagtcg ttccaggacc tgggcctgcc cgacgtgatc gccaacctgt
      601 gcgagaagcc gcgcggcctg gtgctggtca ccggaccgac gggctcgggc aaatcgacga
      661 ccctcgccgc gatgatcgac aagatcaaca acgaggacca cgggcacatc gtcaccatcg
      721 aggacccggt cgagtacctg cacggccaca agtcgtgcgt cgtcaaccag cgggagctgt
      781 tcgccgacac gcactcgttc agcaacgcgc tgaagtcggt cctgcggcag gacccggaca
      841 ccgtactcat cggagagatg cgcgatctcg agaccatcga ggccgcgctg cgcatcgccg
      901 agaccggtca cctgaccttc gcgaccctgc acacgaactc ggcggcgcag acgatcaacc
      961 gcatcatcga cgtcttcccg gcgcaccagc agtcgcagat ccgcgcgcag ctgtcgttcg
     1021 tgctcgaggg gatcctgtgc cagacgctgc tgccgaaggc gtccggcaaa gggcgggcgc
     1081 tggccatcga ggtgatgatc ccgaactcgg ccatccgcaa cctcatccgc gaggacaagg
     1141 tccaccagat ctactcgatg atgcagacgg ggcagtcgca gttcggcatg cagaccttca
     1201 accagtcgct gtcgacgctg tacttcaaga agcagatcag cctgacgacc gccctcagcc
     1261 acagcagcaa cccggacgag ctgcaggaga tgatcaaccg cggcgccggg ctcatcaacc
     1321 agccgacggg gtccggaacc gccgcccgca tggcgaagaa gtaggaaggg ggagagccca
     1381 tgccggtcta cgcctggaag gggaagaaca ggatggggaa gacccaggag gggaccctca
     1441 ccgccgacag caaggacgcg gtgatcgccc tcctgcggcg ccagcagatc atggtcgccg
     1501 cggtcaccga gaaggggaag gagatcgcca tcccgaagtt cggcggtggc gtgaagcaga
     1561 aggaaatcgc catcttcacc cggcagttct cggtcatgat cgatgccggc ctgcccctgg
     1621 tgcagtgcct cgagatcctc ggcggccagc agaagaaccg ggtcttccag aaggtcctct
     1681 tccaggtgcg tcaggacgtc gagtcaggga tgaccctggc cgactcgctg cgcaagcacc
     1741 ctaaagtgtt caacgacctg ttctgcaaca tgatcgccgc cggcgagtcg ggcgggatcc
     1801 tggacaccat cctgcagcgc ctctccacct acatcgagaa gcgggtcaag cttcagagcg
     1861 ccgtgcgctc ggcgatgatc tacccgaccg cggtcatcac catcgccatc ggcgtggtcg
     1921 tggtcatcct ctggaaggtc atcccgacct tcgccatgct gttccagggg ctcggcgcca
     1981 cgctgcccct gccgacccgg atcaccatcg ccgccagccg tttcctcggc cgcttcatgc
     2041 cggtgctggt gatcgggttc gccgccgccg ccttcttcct gcaccgctac taccagacct
     2101 acaaggggcg gcggcagatc gacggcttcg tcctcaagat gccggtgatg ggcgacctga
     2161 tgcgaaaaat cgccgtggcg cggttctgcc gcaccctcgg caccctcatc cagtccggcg
     2221 tgccgatcct cgaggggctg gagatcacgg cacgcaccgc cggcaactcg atcgtcgagg
     2281 acgcgatcat ggcgacccgc aagagcatcg aggaggggaa gacgatcgcc ggcccgctgg
     2341 aggagacgac cgtcttcccg tcgatggtgg tgcagatgat cagtgtcggc gagcagaccg
     2401 gcgcgctcga caccatgctc tcgaagatcg ccgacttcta cgaggacgag gtggacgagg
     2461 cggtggccaa cctgatgtcg ctgctcgagc cgatcctcat cctgttcctg ggtgtcatga
     2521 tcggcggcat cgtcatctcg atgtacatgc cgatgttcag cctgatcggg aagatcgcct
     2581 aggaacgatg gatgccggcg ccgtcgtccg aggcgggacg tcagctgcag tggctcatga
     2641 ccttccgggt cgtgatcacc acgaccctgc tggtctgcac cttcgtcatc gagttgctgt
     2701 accggccgat gctgccgctc ctgccgttct acgtcctggc gggatcggcc tacctgctga
     2761 cgctcgtgta cgccgtcgcc ctgcggaccc ggcgcgccct ccgcttgcag gccttcctgc
     2821 agctcgcggg ggacgcgacg atcgtcaccg ggttcgtcta cctcaccggc ggggcgcaga
     2881 gccccttctc gttcctgtac ctgccgggga tcgtcgccgg ctgcatcctg ctcctgcgcc
     2941 gcggcggctt cgccgtggcg ctcacctcct ggttcctgta cgccgggctg gtgctggtgc
     3001 tgcagcgggg gctcctgccg ccctcgccgc aggcgctctt ccaggagccg tcggtgccgc
     3061 cggtgcggat cgtctacgcg ctcctggcgc acctcgtgtc cttcgtggcg ctggcctacc
     3121 tcgcgtcgca cctcacggag agcctgcggc gcaccggcgc cgagctggag cggcagcacg
     3181 gcgatctgct cgagctgcgg gccctgcacg acaacatcat cgacagcatc aactcggggc
     3241 tcctgacgac cgacgcctcg gggcggatca ccttcgccaa ccgggccgcg gcggagatca
     3301 ccggtcgccc gccggccggc ctggtcgcgg cggacatcgt cgcgctcctg cgccagccgg
     3361 aacgcttcct ccaggagatc cgcgccgcgc tcgatcggcg cagccgccat cgcttcgaga
     3421 tcgactggaa ggacgccatg gggcgggatc tcttcctcgg gttcaccgtc tccgtcctgc
     3481 gcgagcggga cggccggccg gtcgggatga tcttcatctt ccaggacctg acggagatcc
     3541 gcgtcctgga ggaggaggtc gccctcaaga agcgcatggc ggcgctcggc gagatggcgg
     3601 ccggcgtggc gcacgagctg cgcaacccgc tcgcatcgct gagcgggtcg gtgcaggtcc
     3661 tgcagcgtga tctgcgcccg cgcggggagg cgggggaact gatggagatc gtcgtcaagg
     3721 agacgaagcg gctggacggc atcatccgcg acttcttgct cttcgccaag ccgggccgct
     3781 tccatccccg ggaggtcgac gtggcgccgc tcctggcgga cacgctcagc ctcctcgagc
     3841 acagcgacga gcgccgcccg gatcaccgca tcgtgaccga ggccgggtcg gatcccgtgc
     3901 ccgcgtacgt cgacgtcaac cgcatgaaac aggtgttctg gaacctcgcg aagaacgccc
     3961 tcaaggcgat gccgcaggga ggaacgctcg tggcgcgggc ccgccgcggc cccgaggggg
     4021 cccaggtgac gttcacggac agcggcgtcg gcatgagcga ggaggaggtc gcgaagagct
     4081 tccagccgtt ccacaccacc ttcgtcggcg ggtccgggct cgggctgtcg atcgtctacc
     4141 gcatcgtgca ggagcacggc gggcgcatcc aggtgcagag ccgcaagggg gccggaacga
     4201 ccatcaccct ggcgctgccg gcccgagcac cggcgaggga ggtcgcgtgg atcgcatcct
     4261 gatcgtcgac gacgaggcgg gaatgcgcga catgctctcc atcctgctgc gcaaggaggg
     4321 ctacggagtg gcggtcgccg accgggccga gcgcgccatc gagatggtct ccaagggaga
     4381 gttcgacctg gtgatgagcg acatctcgat gccggggaag agcgggctcg aggtcctgcg
     4441 ccaggcgcgc gtcgcctcgc ccgacacgcc ggtgatcctg atcaccgcct acgcctccac
     4501 cgagagcgcc gtcgaggccc tgaagctcgg ggcgtacgac tatctgatca agcccttcga
     4561 cgtcgaggag ctgaagaacg tcgtgcgcaa cgccctcgag aagcggcgcc tcgagaccga
     4621 gaaccgggtc ctcaagcgcg agttgaagca gaagaccggc cccgacacgc tggtcggaga
     4681 gagcccgcgg atgcgcgagg tgctcgcgct cgtcgatcgg atcgcgccga cccactcgac
     4741 cgtcctcatc gtcggagagt cggggaccgg caaggagctg gcggcgcgcg ccatccacgc
     4801 cagcagcccg cgccgcgacc gcccgttcgt gtcgatcaac tgcggggcgc tgccggacga
     4861 gctgctcgaa tcggagctct tcgggcacac gcgcggctcc ttcaccgggg cggtcacctc
     4921 caagaagggg ctgttcgagg tggccgacgg cggcacgatc ttcctcgacg agatcggcga
     4981 cacctccccc gcgatgcagg tgaagctgct ccgggtcctc caggagcggc gcatccgccg
     5041 ggtcggcggg accgacgaga ccgaggtcaa cgtgcgcgtg ctggcggcga ccaaccacga
     5101 tctcgaagcg ctggtgcgtg aaaagcgctt ccgcgaggac ctctactacc ggatcaacgt
     5161 gatccagatc cagatgccgg cgctccgcga gaagcgcgag gacatcccgc gtctcgtgga
     5221 ccacttcgtg tcgaagtact ccgcgctcat gggcaagaag gtctccgggg tgaacgagga
     5281 ggcgcggcgg tcgctgcagc agcacgagtg gccggggaac gtccgcgagc tggagaacgt
     5341 catcgagcgg gcggtggcgc tggcgaacgg cgatgtgctg ggcccggccg acctgtcccg
     5401 cgatctcggc ggggcggccc gcgccgcgaa ggatctgccg gccgagctga cggacgccgg
     5461 catcgacctg gagcgccagc tcgaggggct gcgggagcat ttcatgtcgc tggcgctcga
     5521 ccgcgcgtcc ggggtgcaga cgcgggccgc cgagctcctc ggcatgagct tccgttcctt
     5581 ccgctacttc gcgaagaaat accacctcct cgacggccgg cgggagccgc gcgccggcgc
     5641 ggcgtaggat ccggtcctcg tgatcggcca cgacggccgg cgcggcgcgg tggccgcggg
     5701 gctgctggtc ctccttctcc tcctggcgtt tccggggctg ttcacgggga aggtgttcta
     5761 cctgcgcgat
//
LOCUS       DASRIQ010000558         4902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_751543, whole genome shotgun sequence.
ACCESSION   DASRIQ010000558 DASRIQ010000000
VERSION     DASRIQ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751543"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..91)
                     /locus_tag="VFD06_13815"
     tRNA            complement(1..91)
                     /locus_tag="VFD06_13815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Ser,seq:cga)
     gene            143..595
                     /locus_tag="VFD06_13820"
     CDS             143..595
                     /locus_tag="VFD06_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN04656.1"
                     /translation="MRILVAYDGSPSADLAVEEVAKRPWPAGSSVRIVSVLEDAAMVP
                     AEVGVDLYGPVLEKLRQTQRQALEEGLSRSAARFAGRADLRADTALREGVVVRTLLEA
                     IKDFSADLVVVGSHGAKGFERLFLGSVSHALVTHAPCHVEVIKRPPAA"
     gene            complement(592..1386)
                     /locus_tag="VFD06_13825"
     CDS             complement(592..1386)
                     /locus_tag="VFD06_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN04657.1"
                     /translation="MARRAIPLPVQAGRGFEIAVRERVARLTLRRPPLNILDIVLCRA
                     IAGALRDLARRRDVGVLLLAAEGRSFSAGVDIGEHLPPKAAASLRSFHSVCRALLTFP
                     RPTVARVHGDALGGGLELVLCCDVAVASSSARLGLPEIGLGVFPPLAAALLPRIAGRR
                     AAAEAILWGEAMPAERGLRLGLVSEVVPAADLDAVVERRARRVASLSGAAVALARSAM
                     LRGAEGSFAASLTSVEGIYRRLLRTRDAVEGLQAFLQKRPPDWRHR"
     gene            1458..3215
                     /gene="aceK"
                     /locus_tag="VFD06_13830"
     CDS             1458..3215
                     /gene="aceK"
                     /locus_tag="VFD06_13830"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HZN04658.1"
                     /translation="MSEDPAIRQAADLIHEAFADWRDRFGAITTIARRHFERRDWQAM
                     QRDSARRLDLYGEAVGEAIALLEPVLQERLEDRRLWEPMRDAYAARIRDRGDAGLAET
                     FFNSITRRLFRTIGVEPRVEFVGPPEGGAGPAAGAGVTITFRREGDDPRSLEALARAL
                     LKHYEFAVGWDDFDGDARRIAEVLDRDPTARRLEAIDMALPVFFRGKGAYLVGRARTP
                     AGAVPFLLALVHPDGPDGKVVVDALLLTEDEVSIVFSFARSYFFAEMDRPRDLVEFLA
                     AIMPRKPLAELYNAVGCDKHGKTMLYRTLLHHLETSDDRFVIAPGQRGMVMCVFALPG
                     LDVVFKVIRDQFAAPKTVTHEEVRAKYRLVFRHDRAGRLVDAQEFEHLTFDRARFDPD
                     LLAMLLDLAPSTVSVAGDRVVILHLYTERRLTPLDVYLKSAPLEMARLAVLDYGQVLR
                     DLAATNIFPGDMLPKNFGVSRHGRLIFYDYDELCLLSECRFRYLPAASDDWEEGAAEP
                     SFHVGERDIFPEEFRAFLGFRGELLEEFLKAHGALLDPRFWNEMQERHRRGEVVDIYP
                     YGTARRLRESPGPEGPARL"
     gene            3258..4538
                     /gene="icd"
                     /locus_tag="VFD06_13835"
     CDS             3258..4538
                     /gene="icd"
                     /locus_tag="VFD06_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZN04659.1"
                     /translation="MSDKGEVIALRDGRLQVPARPILPFIEGDGTGPDIWRAAVRVLD
                     AAVAQAYGGKRAIVWKEVLAGQKAYDQTGNWLPDETVDAFRRHLVGIKGPLTTPVGGG
                     IRSLNVALRQVLDLYVCLRPVRWFKGVPSPVKHPEKVDMVIFRENTEDIYAGIEFQAG
                     GDDVKTVLAFLKEKFPSFHRKVRFPESAAIGLKPVSREGSERLIRAALDYALRHKRRS
                     LTLVHKGNIMKFTEGAFRNWGYALAEQEFGDRVVTSEQWEKTRAAKGEEAANAEQKQA
                     IAAGRLLVKDAIADIFLQQVLTRPEEFDVIATLNLNGDYASDALAAQVGGIGIAPGGN
                     INYQTGHAVFEATHGTAPKYANQDRVNPGSVILSGEMMLRYLGWTEAADLVVKGMDGA
                     IAAKSVTYDFARLMEGAHEVRTSEFGDAVIQGMR"
     gene            4552..>4902
                     /locus_tag="VFD06_13840"
     CDS             4552..>4902
                     /locus_tag="VFD06_13840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZN04660.1"
                     /translation="MNILCVQHVPFEGPGSVRPWARARGHELKRVLASDAGPLPGPGS
                     FDWLLLMGGPMGVHDTPKHPWLAAEKDLIRKALDAGRRVLGICLGAQLLAEALGARVF
                     HAKDREIGWFPVQRT"
BASE COUNT          743 a         1708 c         1772 g          679 t
ORIGIN      
        1 tggcggagag ggtgggattc gaacccacgt ggggattgct ccccaacccg ctttcgaggc
       61 gggcccgttg cgaccacttc ggtacctctc cgaagggggg attctatcgc atactgaatg
      121 cctctcccgg aaaggaggcc ccatgcggat cctggtcgcg tacgacggat cgccctcggc
      181 ggacctcgct gtcgaggagg tggcaaagcg tccctggccg gccggttcct ccgtgcgaat
      241 cgtcagtgtg ctcgaggacg ccgccatggt gccggcggaa gtcggggtcg atctctatgg
      301 accggtcctc gagaagctgc ggcagaccca gcggcaggcg ctcgaggagg ggctgagcag
      361 gtcggcggcc cgcttcgccg gccgcgccga cctccgggcc gacacggcgc tgcgcgaggg
      421 ggtggtggtc cggacccttc tcgaggcgat caaggacttc agcgccgatc tcgtcgtggt
      481 cgggtcgcac ggcgcgaagg ggttcgagcg gctcttcctc ggaagcgtca gccatgcgct
      541 ggtgacgcac gccccctgcc acgtcgaggt gatcaagcgg ccgccggccg cctagcgatg
      601 ccgccagtcg ggcgggcgct tctggagaaa cgcctggagt ccctcgacgg cgtcgcgcgt
      661 ccgcagcagc cggcggtaga tcccctcgac cgacgtcagc gaggcggcga acgatccctc
      721 ggcgccgcgc agcatggcgc tgcgcgccag ggcgacggcg gcccccgaga gcgacgcgac
      781 ccgccgcgcc cggcgttcga cgaccgcgtc gagatcggca gccggaacca cctcgctcac
      841 gagcccgagg cgcagccccc tctccgccgg catcgcctcc ccccagagaa tggcttccgc
      901 cgcggcgcgc cgccccgcga tgcgcggaag gagcgcggcg gccagcggcg ggaagacgcc
      961 gaggccgatc tccggcagcc cgagccgcgc tgaagaggac gcgaccgcga cgtcgcagca
     1021 caggaccagc tcgagcccgc cgccgagggc gtcgccgtgg acccgcgcga cggtcggccg
     1081 cgggaatgtc agcagggcgc ggcagacgga gtggaaggag cgcaggctcg cggccgcctt
     1141 gggcggcagg tgctcgccga tatccacccc ggcgctgaag gagcgcccct cggccgcgag
     1201 cagcaggacc ccgacgtcgc gacgccgggc gagatcgcgc aacgctccgg cgatcgcccg
     1261 gcacaggacg atgtcgagga tgttgagcgg cgggcgccgg agcgtgagcc gcgccacgcg
     1321 ctcccggacc gcgatctcga acccccggcc ggcctggacc gggaggggga tcgctcggcg
     1381 cgccatgggc gcggatgtta ccgcgggacg gcggcggcgc ggttcccgga gccgggctgt
     1441 ggcaggatgg cgcgaccatg tccgaggatc cggcgatccg ccaggcggcc gatctcatcc
     1501 acgaggcgtt cgccgactgg cgcgaccgct tcggcgccat cacgacgatc gcccgcaggc
     1561 acttcgagcg ccgcgactgg caggcgatgc agcgcgactc ggcgcgccgg ctcgacctct
     1621 acggcgaggc ggtcggggag gcgatcgcgc tgctcgagcc ggtcctccag gagcgcctgg
     1681 aggaccgcag gctctgggag ccgatgcgcg atgcgtacgc cgcccgcatc cgggaccgcg
     1741 gagatgccgg gctggccgag acctttttca actcgatcac ccggcgcctg ttccgcacca
     1801 tcggcgtcga gccgcgcgtc gagttcgtcg gccccccgga agggggggcc ggcccggcgg
     1861 caggggccgg agtcacgatc accttccggc gcgaggggga cgacccaagg tcgctcgagg
     1921 cgctggcgcg ggcgctcctg aagcactacg agttcgccgt cggctgggac gacttcgacg
     1981 gggacgcgcg ccgcatcgcc gaggtcctcg atcgcgatcc gaccgcgcgc cgcctcgagg
     2041 cgatcgacat ggcgctgccg gtcttcttcc gcggcaaggg ggcgtacctc gtgggacggg
     2101 cgcgcacccc cgccggggcc gtgccgttcc tgctggcgct ggtccatccg gacgggccgg
     2161 acggcaaggt ggtggtggac gcgctcctgc tgaccgagga cgaggtcagc atcgtcttct
     2221 ccttcgcgcg ctcctacttc ttcgcggaga tggaccggcc gcgcgacctc gtcgagttcc
     2281 tcgccgccat catgccgcgc aagccgctcg ccgagctgta caacgccgtc ggctgcgaca
     2341 agcacggcaa gacgatgctc taccgtacgc tcctgcacca cctcgagacg agcgacgacc
     2401 gcttcgtcat cgcccccggg cagcgcggca tggtgatgtg cgtcttcgcg cttcccgggc
     2461 tcgacgtcgt cttcaaggtg atccgcgacc agttcgccgc cccgaagacg gtgacccacg
     2521 aggaggtacg ggcgaagtac cggctggtct tccgccacga ccgggccggc cggctcgtgg
     2581 acgcgcagga gttcgagcac ctcaccttcg atcgggcccg cttcgatccg gacctcctgg
     2641 cgatgctcct cgacctcgcg ccgtccacgg tgagcgtcgc cggggaccgg gtggtcatcc
     2701 tgcacctgta caccgagcgg cggctgacgc cgctcgacgt ctatctgaag tcggcccccc
     2761 tcgagatggc ccgcctcgcg gttctcgact acggccaggt gctgcgcgac ctcgccgcca
     2821 ccaacatctt ccccggcgac atgctgccga agaacttcgg cgtgtcgcgg cacggccgcc
     2881 tcatcttcta cgactacgac gagctgtgcc ttctgagcga gtgccgcttc cggtacctgc
     2941 cggccgcctc ggacgactgg gaggaggggg cggccgagcc gtcgttccac gtcggcgagc
     3001 gggacatctt ccccgaggag ttccgcgcct tcctcgggtt ccgcggggag ctcctcgagg
     3061 agtttctcaa ggcgcacggc gcgctcctgg acccgcggtt ctggaacgag atgcaggagc
     3121 gccatcgccg cggcgaggtg gtggacatct atccctacgg gaccgcccgg cggctccggg
     3181 aatccccagg gccggaaggc ccggcccggt tgtagactct gcacgtccga agcacatcca
     3241 acgaggggag cgatccgatg agcgacaaag gggaggtcat tgccctgcgc gacggccgcc
     3301 tgcaggtccc ggcccggccg atccttcctt tcatcgaagg ggacgggacc gggccggaca
     3361 tctggcgggc cgcggtgcgg gtgctcgacg ccgcggtggc gcaggcctac ggcggcaaga
     3421 gggcgatcgt ctggaaggag gtgctggcgg ggcagaaggc gtacgaccag accggcaact
     3481 ggctgccgga cgagacggtc gacgcgttcc gccgccacct cgtcggcatc aaagggccgc
     3541 tcaccacccc cgtcggcggc ggtatccgat cgctcaacgt ggcgctgcgg caggtcctcg
     3601 atctctacgt ctgcctgcgg ccggtccgct ggttcaaggg ggtcccgtcg ccggtcaagc
     3661 atcccgaaaa agtggacatg gtgattttcc gggagaacac cgaggacatt tacgccggca
     3721 tcgagttcca ggcgggaggc gacgatgtga agaccgtcct cgccttcctg aaggaaaaat
     3781 tcccatcgtt ccaccgcaag gtccgcttcc cggagagcgc cgccatcggg ctgaagccgg
     3841 tgtcgcgcga ggggagcgag cggctcatcc gggcggcgct ggactatgcc ctgcggcaca
     3901 agcggcgcag cctcacgctg gtgcacaagg ggaacatcat gaagttcacc gagggggcgt
     3961 tccgcaactg gggctacgcg ctggcggagc aggagttcgg cgaccgggtc gtcaccagcg
     4021 agcagtggga gaagacgcgc gccgcgaagg gggaggaggc ggcgaacgcc gagcagaagc
     4081 aggcgatcgc ggccgggcgc ctgctcgtga aggacgcgat cgccgacatc ttcctgcaac
     4141 aagtgttgac ccggccggag gagttcgacg tcatcgcgac gctcaacctc aacggcgact
     4201 atgcctcgga cgcgctggcg gcgcaggtcg gcgggatcgg catcgctccg ggcgggaaca
     4261 tcaactacca gaccggccac gccgtcttcg aggcgacgca cggcaccgcc cccaagtatg
     4321 ccaaccagga ccgggtcaac cccggctcgg tgatcctctc gggggagatg atgctgcgct
     4381 acctcggctg gaccgaggcg gcggacctgg tcgtgaaagg gatggacggc gccatcgcgg
     4441 ccaagagtgt cacctacgac ttcgcgcgcc tgatggaagg ggcgcacgag gtgcgcacga
     4501 gcgaattcgg cgacgccgtc atccagggca tgcgctgacc acgctcccgg catgaacatc
     4561 ctctgcgtgc agcacgtccc gttcgagggg cccggcagcg tgcgcccgtg ggcgcgcgcc
     4621 cgcggtcacg agctgaagcg ggtcctcgcg tcggacgccg gcccgctccc cggccccggc
     4681 tcgttcgact ggctgctgct gatgggtggg ccgatggggg tgcacgatac gccgaagcat
     4741 ccctggctcg cggcggagaa ggacctcatc cgcaaagcac tcgacgccgg acgccgggtg
     4801 ctcggcatct gcctcggcgc gcagctcctg gcggaggcgc tcggggccag ggtcttccac
     4861 gcgaaggaca gggagatcgg ctggttcccg gtgcagcgga ca
//
LOCUS       DASRIQ010000559         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1533282, whole genome shotgun sequence.
ACCESSION   DASRIQ010000559 DASRIQ010000000
VERSION     DASRIQ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533282"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1509
                     /locus_tag="VFD06_13845"
     CDS             28..1509
                     /locus_tag="VFD06_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HZN04661.1"
                     /translation="MKGVGRGAVSVAGALAAVVAGALAMAAKDAGDGAMRFEDWSDGA
                     TMRLDYEHSGKAAAGGRPAEEAIALDAFVREGPWPGGRTPQLDPMNLGAYRYEVRDRG
                     THRLLFAAGFGSIYGEWETTDEAKRERRSFHESLRFPFPKRPVQVDLYKRAPDNAWRP
                     LALFLVDPMSPSVRREKPSDRGTVRTLFENGAPERKVDLLILGDGYTAAEMPKFHADA
                     KRLVDTLFSVTPFKERKGDFNVRALDLPSADSGIDRPSDGLWRRTALGTSYDIFGSER
                     YALTFDNRAFRDAAAQAPYEFVEILLNGDTYGGGGIHNLYSTCATGSGSSPYVFVHEF
                     GHHFAALADEYYTSDVAYDTASPIETEPWEPNITALLDPDALKWKDLVTPGTLLPTPW
                     PKQEYEAFSRKIQDRRKALRAAKAPEKEMDALFAEELAWSRPFLAKPPHGGIVGAFEG
                     GGYRAKGLYRPASDCIMFSRNLDRFCPVCARTIEKVIDQSIGR"
     gene            1533..2006
                     /locus_tag="VFD06_13850"
     CDS             1533..2006
                     /locus_tag="VFD06_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04662.1"
                     /translation="MSGDVETLKADLGRWAILLGVAQVTVGCIVGFTPPTAVSWFRGI
                     VMAHIEFAMNGILMIVLGLLTARLRLGRAALWTWFATLQAGTWTNGAAGLAAAFLGAT
                     SPLMTTLNEKFPPPHGTSHPLVTGSLQICGITIMIALLLTLYGLRPSRQPARASA"
     gene            2035..3399
                     /locus_tag="VFD06_13855"
     CDS             2035..3399
                     /locus_tag="VFD06_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN04663.1"
                     /translation="MDVTRRQFVLGTSLTATGVGIAGRTLASPAAPAPAAAKPAPLPN
                     GVAGFKPDDWGSVRAQFRLDPGYAHLSNFYIVSHPKPVRDAIERHRQAFDENPFLYLE
                     QHMFERDEEMLWRKVTAAAADYTGGKPEEIALTSSATMGLALIYNGLRLKPGQEILTT
                     LHDHYSHHESIRLAAAKWGTPVRRTSLYDTGAKANADEIVSRLKAAVTPKTRVVGVTW
                     THSNTGVRLPIRRIADAIAEINHGRDEADRALLVVDGTHAFGAVEEIVASLGCDFMAS
                     GTHKWILAPRGTGIVWAPAARWALLEPTIPTFMDLDVYLAWQDGRVPGPTTAAAVAPG
                     GFFAYEHQWAAVEAFRFHREIGRARIGARIAALNTRIKDGLAKIPKVTLHTPKDPALS
                     AGINCFEVAGLTPQEVVRKLLDRRIVASTSPYKVTYARLSAGIMNTPDEVDRALTAVR
                     AIAA"
BASE COUNT          579 a         1312 c         1224 g          527 t
ORIGIN      
        1 gcgcgtcctc gtgaagatcg tcggcgcatg aagggcgtcg ggcgcggggc ggtctcggtg
       61 gcgggcgcgc tcgccgccgt ggtggcggga gcgttggcga tggcggcgaa ggacgccgga
      121 gatggcgcga tgcgcttcga agactggtcg gacggcgcga cgatgcggct cgactacgag
      181 cactccggga aggccgcggc cggcggccgg ccggcggagg aggcgatcgc cctcgacgct
      241 ttcgtgcgcg agggcccctg gccgggcggc cgcacgccgc agctcgaccc gatgaacctc
      301 ggcgcctacc ggtacgaagt gcgcgaccgt gggacacacc ggctcctgtt cgccgccggc
      361 ttcggcagca tctacggcga gtgggagacg accgacgaag cgaagcgcga gcgccgctcg
      421 ttccacgagt cgctccgttt tccgttcccg aagcgaccgg tacaggtcga tctctacaag
      481 cgtgctccgg acaatgcctg gcggccgctg gcgctgttcc tcgtcgatcc catgtctccg
      541 tccgtcaggc gtgagaagcc gagcgaccgc ggcaccgtcc ggaccctgtt cgagaacggc
      601 gcgcccgagc gcaaggtcga tctcctcatt ctcggggacg gctacaccgc cgccgagatg
      661 ccgaagttcc acgccgacgc caagcggctg gtggacacac tcttcagcgt gacgccgttc
      721 aaggagcgca agggggattt caacgtgcgc gccctcgatc tcccgtcggc cgactccggc
      781 atcgaccggc cgagcgacgg gctgtggcgc cgcaccgcgc tcgggacctc ctacgacatc
      841 ttcggatcgg agcgctacgc tctgaccttc gacaaccggg cgtttcgcga cgccgcggcg
      901 caggcgccct acgagttcgt cgagatcctc ctcaacggcg acacctacgg cggaggcggc
      961 atccacaacc tttattccac ctgcgccacc ggcagcggct cgagcccgta cgtcttcgtc
     1021 cacgagttcg gccatcactt cgccgccctc gccgacgagt actacacgtc ggacgtcgcc
     1081 tacgacaccg cctctcccat cgagaccgaa ccctgggagc cgaacatcac ggcgcttctc
     1141 gacccggacg cgctcaagtg gaaggacctg gtcacccccg gcaccctcct tccgacgccg
     1201 tggcccaagc aggagtacga agccttctcg aggaagatcc aggatcgcag gaaggcgctg
     1261 cgcgccgcca aggcgcccga gaaggagatg gacgcgctgt tcgccgagga gctggcctgg
     1321 agccggccgt tcctggcgaa gccgccgcac gggggcatcg tcggagcgtt cgaggggggc
     1381 ggctaccggg cgaaaggtct ctaccgtccg gcgagcgact gcatcatgtt cagccgcaac
     1441 ctcgaccgct tctgcccggt ctgcgcccgc accatcgaga aggtgatcga tcagagcatc
     1501 ggccggtgac ggccttccag ccgaggcgaa gcatgtccgg tgatgtcgaa actttgaagg
     1561 cggatctggg acgctgggcc atcctgctcg gcgtggcgca ggtcacggta ggatgcatcg
     1621 tggggttcac cccccccacc gcggtgtcgt ggttccgcgg catcgtgatg gcgcacatcg
     1681 agttcgcgat gaacggcatc ttgatgatcg tcctcggtct cctgaccgca cggctccgac
     1741 tcggccgggc ggcgctgtgg acctggttcg cgaccctgca ggccggcacc tggacgaacg
     1801 gcgccgcggg gctcgcggcg gcgttcctcg gcgccacgtc gccgctcatg accactctca
     1861 acgagaagtt cccgccgccg cacgggacct cgcacccgct cgtcaccggg tcgctgcaga
     1921 tctgcggcat caccatcatg atcgccctgc tgctcaccct ctacgggctg cgtccctcga
     1981 ggcagccggc gcgggcaagc gcatgacccg ccgcggaacg acaggagatt cgacatggat
     2041 gtgacccgcc gtcagttcgt gctcggaacc agtctcaccg ccaccggcgt cggcatcgcg
     2101 ggccggaccc tggcgagtcc ggccgccccg gcgccggcgg ccgcgaagcc ggcgccgctc
     2161 cccaacggcg tcgccgggtt caagccggac gactggggct cggtgcgggc gcagttccgg
     2221 ctcgacccgg gctacgcgca tctgtcgaac ttctacatcg tctcccaccc gaagccggtg
     2281 cgggacgcca tcgagcgtca ccggcaggcg ttcgacgaga acccgtttct ctatctcgag
     2341 cagcacatgt tcgagcggga cgaggagatg ctctggagga aggtgaccgc ggccgcggcg
     2401 gactacaccg gcgggaagcc ggaggagatc gccctgacca gcagcgccac gatggggctc
     2461 gccctcatct acaacggcct gcgcctgaag ccgggacagg agatcctcac gaccctccac
     2521 gaccactatt cgcaccacga gtcgatccgg ctcgccgccg cgaaatgggg aacgccggtg
     2581 cggcgcacct cgctctacga caccggagcg aaggcgaacg ccgacgagat cgtgtcccgc
     2641 ctcaaggcgg ccgtcacgcc gaaaacgcgc gtcgtcggcg tcacctggac ccactcgaac
     2701 acgggggtgc gcctcccgat ccgccgcatc gccgacgcga tcgcggagat caaccacggt
     2761 cgcgacgagg cggaccgcgc cctgctcgtc gtggacggga cgcacgcctt cggcgccgtc
     2821 gaggagatcg tggcgtcgct cgggtgcgat ttcatggcca gcggcaccca caagtggatc
     2881 ctcgcgccgc gcggcaccgg catcgtctgg gcccccgcag cgcgctgggc cctcctggag
     2941 ccgacgatcc cgaccttcat ggacctcgat gtgtacctgg cgtggcagga cggccgcgtt
     3001 cccggtccga cgaccgccgc ggcggtggct cccggcgggt tcttcgccta cgagcaccag
     3061 tgggcggcgg tcgaggcgtt ccgcttccac agggagatcg gccgggcccg catcggcgcc
     3121 cgcatcgccg cgctgaacac ccggatcaag gacgggctgg cgaagatccc caaggtcacc
     3181 ctgcacactc cgaaggaccc ggcgctttca gccggcatca actgcttcga agtggcgggg
     3241 ctcacccctc aggaggtggt gaggaagctc ctcgaccgcc gcatcgtcgc cagcacctcg
     3301 ccgtacaagg tcacctacgc gcggctctcc gccggcatca tgaacacgcc ggacgaggtg
     3361 gaccgggcgc tgactgcggt gcgggcgatc gcggcctgac cttcgcgcgg gcgcgcgacc
     3421 ggccgcgcgc cggggcgacc agccgaagcc gatgagtccg ccgatctgcc agtagtcggc
     3481 gtcgccgtcg atgcacaaac ttgacggcgg gatcgcgccg gcggcgagcc gcgccggaac
     3541 cgggcgcgcc cgccctcggt gatttacctt acagtcgcgc gcgacagccc gcgataggat
     3601 ttcccgtaac tcaaaaccat ccgtcacgcc ggaaccgtcc tc
//
LOCUS       DASRIQ010000560         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_235059, whole genome shotgun sequence.
ACCESSION   DASRIQ010000560 DASRIQ010000000
VERSION     DASRIQ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235059"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFD06_13860"
     CDS             <1..527
                     /locus_tag="VFD06_13860"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN04664.1"
                     /translation="ADFRMDADAKADAGSRSPGVVMSDLWITSATKMKLAADSRTPAT
                     EINVDTMDGVVTLFGMVPNQESKSAAQEVVQSVSGVKQVENKLEIVTAEKQDMVQARD
                     EEVQESVKKALKDRGDQDNANIGVEVSNGVVRLTGKVPSWERSLSAVYAARSVQGVRS
                     VRNDMTVETQQAAR"
     gene            757..2823
                     /locus_tag="VFD06_13865"
     CDS             757..2823
                     /locus_tag="VFD06_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN04665.1"
                     /translation="MKLQARMKPRSRRGGGGRAHDDAPIGLALISSEWRFLQVNPRLC
                     TLLGYTRKQLLSRTLGAVIDPLDQQLMESNSRRLLAGEIDEFQSEQHFLHADGRHFPG
                     RFSASVTTGVGNRSFACVLEIDAPGERGASRTQKNSREAFTVLDDFFDGVLAADESGR
                     IACFNQAAGRLFGYESAEVVGREAGLIIAGPCQEEFADFLAGWASSGWEAAPSRPREM
                     WGRRKDGSTFPIEIRASRMILGGEPHLVAILRDISEQKAQTEALEYQTLHDVLTNLPN
                     RTLLNDRLHQAILTGSRQRRTAALLILDVDGFKEVNDTCGHHTGDLLLQQIALRLEGL
                     LRSSDTVARLGGDEFAILPGLGIGGEDGARTAQKVLRVLEQPFMIDGRVLRISASIGI
                     ALFPAHGRDASSLMRHADEAMYVAKRARSGYAFHVPRQHAVIPEHRVQRGELGHAIEH
                     DQMVLHFQPTIDLRIGRTIGVEALVRWQHPEQGLLLPMSFIPVAEEAGLIMPLTRWVL
                     NRALRQARAWSKAGLDIDVAVNLSAGSLLDAGLPAMTSDLLKVWRFDPGRLKADLSES
                     SVMAAPAVETATRLGAMGIGLAIDDFGAGAASLLHLARLPVREVKIDGARGDDSTLRP
                     IVDQGHRMGFRMAAKRVEDQATLDRLRGLGCDSAQGFHLCPPIVAADVAPWLRDSAWG
                     LAARTQ"
BASE COUNT          509 a         1021 c         1057 g          458 t
ORIGIN      
        1 atgccgattt caggatggat gcggacgcga aagcggatgc cgggagccgg agtccgggcg
       61 tcgtcatgag cgacctctgg atcacctcgg ccaccaagat gaagctggcg gctgactcac
      121 gcacccccgc gaccgagatc aacgtcgaca ccatggatgg ggtcgtgacg cttttcggga
      181 tggtccccaa ccaggaatcg aagtcggcgg ctcaggaggt cgtccagagc gtgtccggcg
      241 tgaagcaggt ggagaacaag ctggaaatcg tgaccgccga gaagcaggac atggtccagg
      301 cgcgtgatga ggaagttcag gagagcgtca agaaggcgct caaggatcga ggcgaccagg
      361 acaacgccaa catcggcgtc gaagtgagca acggcgtcgt gcgtctgacc ggcaaggtcc
      421 cttcgtggga gcgcagcctg tcggccgtct acgcggcccg ttccgtgcag ggggtccgat
      481 ccgtccgcaa cgacatgact gtggagaccc agcaggccgc ccggtagcct cggcgcggtc
      541 aatgaggcag cggcgggcgg ccccaccgcc cgccgcccgc cgcatccctg ggagctgccg
      601 cgcctcctcc ctcgaagtca ggacgctcac tccttccgtt catcaccccg ttgcgggttc
      661 ccctgtcagg acactccgga ccggcgattc atggatctgc cgaattgtgg gagcctcgcg
      721 agcggggcta taaggcaccg tgacatcgga ggtggggtga aactgcaggc ccggatgaag
      781 ccccgttcga ggcgcggggg gggcgggcgc gcacacgacg acgctcctat cggcctcgcg
      841 ctgatcagtt ccgagtggcg attccttcag gtcaacccga gactctgcac cctcctgggc
      901 tacacgagaa agcagctcct gagcaggacg ctgggagcgg tcatcgatcc gctggaccag
      961 cagctcatgg agtcgaactc ccgccggctc ctggcgggcg agatcgacga attccaatcg
     1021 gaacagcatt tcctccacgc cgacggtcgg cactttccgg gccgattcag cgcctccgtc
     1081 acgaccggcg tcgggaaccg ctccttcgcc tgcgtgctcg agatcgacgc accgggggag
     1141 cgcggggcat cccgcacgca gaagaacagc cgcgaggcct ttacggtcct ggacgatttc
     1201 ttcgacggcg tcctcgcggc cgacgagagc ggccgcatcg cctgcttcaa ccaggcggcg
     1261 ggcaggctgt tcggctacga atccgccgag gtcgtgggcc gcgaagccgg cctgatcatc
     1321 gccgggccct gccaggagga gttcgcggac ttcctcgccg gctgggcgag ctccggttgg
     1381 gaagccgccc catcgagacc gcgcgagatg tggggccggc gcaaggacgg gtccaccttt
     1441 ccgatcgaga tccgcgccag ccggatgatc ctggggggcg agccgcatct cgtcgccatc
     1501 ctgcgggaca tctccgagca gaaggcccag accgaggcgc tcgagtacca gacccttcac
     1561 gatgtcctga ccaaccttcc gaaccggacc ctgctgaacg atcggctgca ccaggcgatc
     1621 ctgacaggga gccggcaacg caggacggcg gccctcctga ttctggacgt ggacggtttc
     1681 aaggaggtga acgacacctg cggccaccac acgggggact tgctcctgca gcagatcgcc
     1741 ctgcgcctgg aaggcctcct caggagctcc gatacggtgg cccggctcgg cggagacgag
     1801 ttcgccattc tccccggcct cggcatcggg ggcgaggatg gagcgaggac cgcgcaaaag
     1861 gtcctccggg tgctggaaca gcccttcatg atcgacggtc gagtcctccg catcagcgcc
     1921 agtatcggca tcgcgctgtt ccccgcgcac ggacgggatg cgtcgagcct gatgcgccac
     1981 gccgacgagg cgatgtacgt ggccaagcgc gcgcgcagcg gctacgcgtt ccatgtcccg
     2041 cggcagcacg ccgtgatccc cgagcaccgc gtgcagcgcg gcgagctggg gcacgccatc
     2101 gaacacgacc agatggtgct gcatttccaa cccacgatcg atctgcggat cggcaggacg
     2161 atcggcgtgg aggcgctggt gcgctggcag cacccggaac aggggctgct gctcccgatg
     2221 agcttcatcc ccgtcgcgga ggaggcgggg ctcatcatgc ccctgacgcg gtgggtgctc
     2281 aatcgggctc tgcggcaggc ccgggcctgg agcaaggccg gtctcgacat cgacgtcgcg
     2341 gtgaatctct cggcggggag cctgctggat gcgggcctgc cggccatgac cagtgatctc
     2401 ctgaaggtct ggaggttcga tccgggcagg ctgaaggcgg atctctctga gagcagcgtc
     2461 atggcggccc cggcggtcga aacggcgacg cggctcggcg ccatggggat cggactcgcc
     2521 atcgacgact tcggcgccgg tgcggcgtcg cttctgcatc tggcacgtct gcccgttcgg
     2581 gaggtcaaga tcgatggggc ccggggcgac gacagcaccc tccgccctat cgtcgaccag
     2641 gggcacagga tgggattcag gatggccgcg aaacgcgtcg aggatcaggc gaccctggat
     2701 cgcctgcgcg ggctcggctg cgactcggcg cagggcttcc acctctgccc gccgatcgtc
     2761 gcggccgacg tcgccccctg gctgcgggac tccgcctggg gactcgcggc gcgcacgcag
     2821 taggtcgatc ccggacgatc ccgccggtcc cgcgaatcgt cgctccgcct cgtcgcgctc
     2881 agggccgatg gtgcgcgacc ggagacagcg ggatgcgcgc cgtcacctcg gtcccggcgc
     2941 catgggagga cctgatctcc agggtgccgc ccacgcaggc ggcgcgctcg cgcagaccga
     3001 tgaggcccgt ccccttcccc ggcggtgagt cgcgatcgga gtcga
//
LOCUS       DASRIQ010000561         4440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3094579, whole genome shotgun sequence.
ACCESSION   DASRIQ010000561 DASRIQ010000000
VERSION     DASRIQ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3094579"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFD06_13870"
     CDS             complement(<1..836)
                     /locus_tag="VFD06_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HZN04666.1"
                     /translation="MFETLTGRLQTVFRTLRGHGHLTEVELKDGLRALRLALLEADVH
                     VDVVKTVIEKVRAQAGEQEILKSLSPGQQIVKLVRDQLLAALEQGTEARLREGQPPSV
                     VLMTGLQGSGKTTTTGKLALWLKGKGRSPLLVAADVQRPAAVEQLQTLGKRVEVPVVT
                     APPGGSAADAARAGLREARLTGRTPVLVDTAGRLHIDDALMQELQALKDLTKPSEILY
                     VADAMTGQDAVRSAAAFHGRLGITGAILTKADGDARGGAALSLRHVTGVPIKFVGMGE
                     HLP"
     gene            complement(863..2038)
                     /locus_tag="VFD06_13875"
     CDS             complement(863..2038)
                     /locus_tag="VFD06_13875"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZN04667.1"
                     /translation="MADIAAGRFVIVVDDEDRENEGDLTMAAEKVTPEAINFMARFGR
                     GLICLSMTEDRLRELDIPLMVQKNTSPYGTAFCVSIEARDQVSTGISAADRARTVRVA
                     IDSDTRPSDLTRPGHMFPLRAQNGGVLKRAGQTEAAIDLARLAGLSPAGVICEIMNED
                     GTMARQADLEAFSALHGIRRITIADLISYRMRHERLVRRVAQPWLPTRYGRFRLVAYR
                     SDIEHKTHLALVLGTIAPGDRVLVRVHSECITGDVFGSMRCDCGPQLQRAMEIIAAEG
                     KGVIVYLRQEGRGIGLLNKLKAYELQEEGKDTVEANHKLGFKADMRDYGIGAQILRDL
                     GCSRIRVLSNNPGKFTALRGYGLEIVERVPLEVAPTETSRQYLKTKKEKLGHLLSLV"
     gene            complement(2110..2778)
                     /locus_tag="VFD06_13880"
     CDS             complement(2110..2778)
                     /locus_tag="VFD06_13880"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZN04668.1"
                     /translation="MFTGIVETKGRIEAAEPQGAERRLRVAAPFARELTRGGSVAVDG
                     VCLTMTDSGDGWFEAALGGETVARTTLGSLRPGDEVNLERPLRAGDPLGGHLVQGHVD
                     AVVRVTERRQDGPGARLRVSLPEALGPLVVAQGSVTLDGVSLTVALRDDDGFEVMLIP
                     ETLRVTTLGRRRAGDALNLEVDLIGRYVAAAVAARTPSRGSAITREFLARHGFAPAVE
                     EVRS"
     gene            complement(2803..3918)
                     /gene="ribD"
                     /locus_tag="VFD06_13885"
     CDS             complement(2803..3918)
                     /gene="ribD"
                     /locus_tag="VFD06_13885"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZN04669.1"
                     /translation="MVRRKGDDHRWLRLALRLAERGRGRTRPNPMVGAVVVRGERVVG
                     RGWHARAGSPHAEARALRQAGTKARGARLYVNLEPCCHFGRTPPCTDAILASGVREVI
                     ACMRDPDPRVGGKGFAALRKSGVRVRIGALRNEAERLNAPYLRRQRSGLPSVTLKAGM
                     SLDGRIATRAGRSRWITSSAARREARRLRTRHDAVLVGVGTLLADDPRLSGGAANGHP
                     QPVRVVLDSRLRTPPGSKILRSPGGPVLLLARRDASKTRRAKLERAGATIVEVGSRRG
                     RVKLRAGLEALGRRGISSVLIEGGGEVLGAALDEKVGDRLVLYVAPRLLGGSGARPAF
                     AGRGAAPVEGAARLVAPRWRRLAGDWVIEAGLDHRRR"
     gene            complement(3947..>4440)
                     /locus_tag="VFD06_13890"
     CDS             complement(3947..>4440)
                     /locus_tag="VFD06_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZN04670.1"
                     /translation="GAGEQLRIWAARAGAEFSGERPGADPSAVAFDAAAAAEARGCNV
                     LIVDTAGRLHTQDPLMRELEKVRRVLGRRIAGAPHEVLLVLDATTGQNGLPQAQRFLA
                     AAGVGGLVLTKIDGTARGGIAVRIVQELGLPLRYLGVGEGADDLVDFDPEAFVDGLLP
                     AAA"
BASE COUNT          577 a         1762 c         1478 g          623 t
ORIGIN      
        1 gggaggtgct cccccatgcc gacgaacttg atcggcacgc cggtcacgtg ccgcagcgac
       61 agggcggcgc caccgcgcgc atcgccgtcc gccttggtca ggatcgcacc ggtgatcccc
      121 aggcgcccgt ggaacgccgc ggcggatcgg accgcgtcct gcccggtcat cgcgtcggcg
      181 acgtagagga tctcggacgg tttcgtcagg tccttgagcg cctgcagctc ctgcatcagc
      241 gcgtcgtcga tgtgcagccg ccccgcggtg tcgacgagga ccggcgtgcg cccggtgagg
      301 cgcgcctcgc gcagcccggc ccgggcggcg tcggcggcac tcccgcccgg cggggcggtg
      361 acgaccggga cctcgacgcg cttcccgagc gtctgcagct gctcgaccgc cgccggccgc
      421 tgcacgtcgg cggcgaccag gagcggggag cgccccttcc ccttcagcca cagcgccagc
      481 ttgccggtcg tggtcgtctt gcccgacccc tgcagcccgg tcatgaggac gaccgacggc
      541 ggctgccctt cgcgcaggcg cgcctcggtg ccctgctcca gcgccgcgag cagctggtcg
      601 cgcaccagct tgacgatctg ctgacccgga ctcaggctct tgaggatctc ctgctccccc
      661 gcctgcgcgc gcaccttctc gatgacggtc ttcacgacgt ccacgtgcac gtccgcctcg
      721 agcagggcca agcgcagcgc gcgcagaccg tccttcagct ccacctccgt gaggtggccg
      781 tgcccccgca gggtgcggaa gaccgtctgc agccgtccgg tcagggtctc gaacatcgct
      841 cgcgtcccgg gaggcgccgc gctcagacga gcgacagcag gtgtcccagc ttctccttct
      901 tggtcttgag gtactgccgg gaggtctcgg tcggcgccac ctcgagcggc acgcgctcga
      961 cgatctccag tccgtagccg cgcagcgccg tgaacttccc cgggttgttg ctcaggaccc
     1021 ggatgcgcga gcagccgagg tcccggagga tctgcgcccc gatgccgtag tcgcgcatgt
     1081 ccgccttgaa gccgagcttg tggttcgctt cgaccgtgtc cttcccttcc tcctggagct
     1141 cgtacgcctt gagcttgttc aggagcccga tgccgcgccc ttcctgccgc aggtagacga
     1201 tgaccccctt cccctcggcc gcgatgatct ccatcgccct ctgcagctgt gggccgcagt
     1261 cgcagcgcat cgatccgaag acgtccccgg tgatgcactc cgagtgcacc cgcaccagca
     1321 cgcggtcgcc gggggcgatg gtcccgagca cgagcgccag gtgcgtcttg tgctcgatgt
     1381 cgctgcggta cgcgacgaga cggaagcgcc cgtagcgggt cggcagccac ggctgcgcca
     1441 cgcgccggac cagacgctcg tgccgcattc ggtaggagat gaggtcggcg atggtgatgc
     1501 ggcggatccc gtgcagggcc gagaacgcct cgaggtccgc ctggcgggcc atggtgccgt
     1561 cctcgttcat gatttcgcag atcacgccgg ccggggacag accggcgagc cgcgccaggt
     1621 cgatcgccgc ctcggtctga ccggcccgct tcaggacgcc gccgttctgc gcacgcagcg
     1681 ggaacatgtg cccggggcgg gtcagatcgg aggggcgggt gtccgaatcg atggcgacgc
     1741 gcacggtgcg ggcgcgatcg gcggcggaga tcccggtgga cacctggtcg cgcgcctcga
     1801 tcgagacgca gaacgccgtg ccgtaggggg aggtgttctt ctgcaccatg aggggaatgt
     1861 cgagctcgcg cagccgatcc tcggtcatcg acaggcagat cagcccccgc ccgaagcgcg
     1921 ccatgaagtt gatcgcttcg ggggtcacct tctccgccgc catggtgagg tccccctcgt
     1981 tctcccgatc ctcgtcgtcg acgacgatca cgaaacggcc ggcggcgatg tcggcgatcg
     2041 cttcctcgac cgacgcgtag gcgtcgaacc gcctggaccg cctggtgacg cgcttcccct
     2101 tggaacgctt cacgaccgga cctcctccac cgccggcgcg aatccatgcc gcgccaggaa
     2161 ctcccgggtg atcgcggatc cgcgcgacgg cgtccgcgcg gcgaccgccg ccgcgacgta
     2221 ccggccgatc agatccactt ccaggttcag cgcgtccccg gcgcggcgtc gccccagggt
     2281 cgtgacgcgc agcgtctccg ggatgagcat cacttcgaaa ccgtcgtcgt cgcgcaatgc
     2341 aaccgttaaa ctgacgccgt cgagggtcac cgatccctgc gccacgacga gcggcccgag
     2401 cgcctccggg aggctcaccc gaagccgcgc gccgggaccg tcctggcggc gctccgtcac
     2461 ccgcaccacc gcgtccacgt gcccctggac gaggtgtccg ccgagcggat ctccggcgcg
     2521 cagcgggcgc tcgaggttga cctcgtcccc cgggcggagg gagccgagcg tcgtgcgggc
     2581 caccgtctcc ccgccgagcg ccgcctcgaa ccagccgtcc cccgaatccg tcatggtcag
     2641 gcagacgccg tccacggcga cgctgccgcc gcgcgtcagc tcacgcgcga acggcgcggc
     2701 gacgcgcagg cggcgctccg ctccctgcgg ctccgccgcc tcgatgcgtc ccttcgtttc
     2761 cacgatcccg gtgaacatcc tcttcgctcc gccgcgtcgc tctcagcgcc gccggtggtc
     2821 cagccccgct tcgatcaccc agtcccccgc gagccgccgc cagcgcggcg ccaccagccg
     2881 ggccgcccct tcgaccggcg ccgcaccgcg cccggcgaac gccggcctcg cgcccgatcc
     2941 tccgagaagc cggggcgcca cgtacagcac cagccggtcc ccgaccttct cgtcgagcgc
     3001 cgctccgagc acctcgcctc ccccttcgat caggaccgac gagatgccgc gccggccgag
     3061 ggcttcgagc ccggcccgca gcttcacgcg gccgcggcgc gacccgacct cgacgatcgt
     3121 cgctcccgcc cgctccagct tcgcgcggcg cgtcttcgag gcgtcgcgtc gcgccagaag
     3181 gagcaccggc cccccggggc ttctcaggat cttcgacccc ggaggggtcc gcagccggct
     3241 gtccagcacg acccgcaccg gctgagggtg cccgttggcc gcgccgccgc tgagccgcgg
     3301 atcgtccgcc aggagcgtcc ccactcccac caggaccgcg tcatggcgcg tccggagccg
     3361 ccgcgcctcg cgccgcgccg ccgaggaggt gatccaccgc gatcgaccgg cccgcgtcgc
     3421 gatccgccca tccaggctca tgccggcctt cagcgtcacg ctcggcagac cgctcctctg
     3481 gcgccgcagg tagggcgcgt tgagccgctc cgcttcgttc cgcaacgctc cgatcctcac
     3541 cctcaccccc gacttcctca gtgccgcgaa ccccttgccg ccgacccgcg ggtcggggtc
     3601 ccgcatgcag gcaatcacct cccgcacccc ggacgccaga atcgcgtcgg tgcagggagg
     3661 ggtccgcccg aaatggcagc agggctcgag gttcacgtac agccgcgcgc cccgcgcctt
     3721 cgtcccggcc tggcgcagcg cccgcgcttc cgcgtgcggc gagccggccc gcgcgtgcca
     3781 gccacgcccg accacccgct cgccgcgaac cacgaccgca ccgaccatgg gattggggcg
     3841 ggtgcggccg cgcccgcgct ccgccagccg gagcgccagc cgcagccagc gatgatcgtc
     3901 ccccttccgc cttaccacgg ccacgcctcg ccgcacccgc gccgcgttag gcggcggccg
     3961 gcaggagtcc gtcgacgaac gcctccggat cgaaatcgac caggtcgtcg gccccttcgc
     4021 cgaccccgag atacctgagc ggcagcccca gctcctgcac gatgcgcacc gcgatgccgc
     4081 cgcgcgcggt gccgtcgatc ttggtgagca ccagcccgcc caccccggcg gcggcgagga
     4141 agcgttgcgc ctgcggcagc ccgttctgcc cggtggtggc gtcgaggacg aggagcacct
     4201 cgtgcggggc gccggcgatg cggcggccaa gcacccggcg gaccttctcc agctcccgca
     4261 tcagcgggtc ctgcgtgtgc agccgcccgg cggtgtcgac gatgaggacg ttgcagccgc
     4321 gcgcctccgc tgccgcggcg gcgtcgaacg ccaccgctga cggatcggcc ccgggacgct
     4381 cgccgctgaa ctcggccccg gcgcgcgccg cccagatccg cagctgctct cccgcaccgg
//
LOCUS       DASRIQ010000562         7245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_991364, whole genome shotgun sequence.
ACCESSION   DASRIQ010000562 DASRIQ010000000
VERSION     DASRIQ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991364"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFD06_13895"
     CDS             <1..227
                     /locus_tag="VFD06_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04671.1"
                     /translation="PIPALNTALEPYIPAIKTFPQACIECHKLALNNNGAALGESFLP
                     FRACPEKPQPGQQLPANCTPGKVNTLAAHH"
     gene            complement(235..654)
                     /locus_tag="VFD06_13900"
     CDS             complement(235..654)
                     /locus_tag="VFD06_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZN04672.1"
                     /translation="MKSYRKELWFNVPERRAYLNITPQVNECLRDSGIGEGLCLVNAM
                     HITASVFINDDEPGLLRDFEEFLERLAPHAPTSTYRHNLTGEDNGDAHIKRQLFGREV
                     VVAVTGGKLDFGPWEQIFYGEFDGRRPKRVLVKVIGE"
     gene            739..1995
                     /locus_tag="VFD06_13905"
     CDS             739..1995
                     /locus_tag="VFD06_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN04673.1"
                     /translation="MTTSTGTEDPRAIREAARALRPFIVDLTLRICRVRTVNFMPDDF
                     PGGGPDDMPGPGMESRVTSILGEECRRLKIPFTTHAKVEGRESLLATVGQKQPGYRHM
                     LVLLHTDTVPAGSPTVWKRPPFEPVEEGGTLYGRGVLDDKGPLAASFAALRLLKERED
                     RIPGAFTFGAVADEEVGIGVGLPFLIEQGLITCTDAVIPDIAGNMKEINIAEKGRALL
                     RIATRGKQAHAMEPAKGVNAILALARALLAVERMTLEHRPHPLLGGPTINPGLARGGV
                     APNAVPADAQAILDIRYVPGQTAEGIRSEIAAAASKAIADLPGAAVEVEIVQEAKPCE
                     VSPDAPIVRRLLRHAPEAKVIGSGGGTFAKDLVLMGVDAVGWSAGDEETYHQPNEEID
                     VEQLVTFAGRLAALALEIGSEKVEGS"
     gene            1992..2798
                     /locus_tag="VFD06_13910"
     CDS             1992..2798
                     /locus_tag="VFD06_13910"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN04674.1"
                     /translation="MRSVIALGLGVLLLVGLVFVLLACARGEAGAPGQPARRAEVPAA
                     APTDAAAGHHAHGAAAGPDAAADHATVHHAFNDPERWAKVFDDPARDAWQKPVEVVAS
                     LGLRKGMTVADLGAGTGYFTAALARAVGPTGVVLSIDPEPEMVEYLGRRAHREGLANV
                     VPVLALYEDPFLPPGRVDRVLIVDTYHHIDDRQQYFRRVGDDLAPGGRVAVVDFHKRP
                     LPVGPPPEAKLDREVVVAEMQKAGYRLAEEPTFLPYQYYLVFEPIRGAPQ"
     gene            2795..3376
                     /gene="efp"
                     /locus_tag="VFD06_13915"
     CDS             2795..3376
                     /gene="efp"
                     /locus_tag="VFD06_13915"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZN04675.1"
                     /translation="MIVTSDFKRGLAILVEGKPFIITEYSVQTPSARGAATLVRVKAR
                     NVLTGQVLDMTFKSGDKFDEPDLEYRKTSFLYADGDEYHFMDETSYEQFHLSKETIGD
                     DARWLKDGVSVRAVTFDGRVYGIELPQFVEFEVIETGPGGRSDMASGKVMKPATLSNG
                     TQIRVPVYLDAGEKVMVDTTTGEFVRRVSEEKR"
     gene            3373..3705
                     /locus_tag="VFD06_13920"
     CDS             3373..3705
                     /locus_tag="VFD06_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04676.1"
                     /translation="MKISRRVVGRMILGTPALLAGAGLMGTLGQGARADEPQASPAPE
                     PEAEPTPLAKFIAKQEEGLSGAERDKVRKDVTQLEEALKVVRDFPIANDIPPAGGFRP
                     MRSVRKGR"
     gene            3716..5137
                     /locus_tag="VFD06_13925"
     CDS             3716..5137
                     /locus_tag="VFD06_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN04677.1"
                     /translation="MPTPNLSEETLFLPVTDLAARLHSRRLSPVALTEAYLARIDELD
                     PAYHAWVTVMRDSALAEAKQAEREIARGRIRGPLHGIPYGAKDLVATKGTRTTWGAKP
                     YEQQSFDYDAAVIRKLRGAGAILLGKLAMIEMAGGLGYEKGTASLTGAARNPWDPSRW
                     TCGSSSGSGAAVGTGMLPFAIGSETWGSIVCPSAFCGITGLRPTFGRISRQGAMALSW
                     TLDKLGPMGRTARDCEVVYRVLSGRDPDDPYTTDEPLGPESSSAGAQRLKVGFLRLDF
                     KKSGNPSVEHAFMKALDDLRGAGVAAEETRLPELPFEAATAVILTSEVATAYEDLQRG
                     DKVRQLSSPGAALAFVTAHAVRGSDYVKAERLRTVCVRAMADLFSKYDVILYPTEMHT
                     AFGYDQDFSGVSWSDPAGGAGNLCGLPAVSVPCGFADDGLPAGLGVLAGAYDEAKAVT
                     LARHYQSITSWHTRRPPVTSART"
     gene            5167..5904
                     /locus_tag="VFD06_13930"
     CDS             5167..5904
                     /locus_tag="VFD06_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN04678.1"
                     /translation="MDPAGKVALLTGTARIGRTVAETLARRGADVAISWRTSRDSAEE
                     TADRVRAAGRRAVTIQADLSDPQAARAIVDEVGRDLGGPDILVPMASHYEKVPFDLLD
                     DAAWRRNVDADLQSTYRLALLAARPMRERGGGRIVAFADWLPRSGRPRYKGFLPYYVA
                     KAGVIGLIEALALELAPSILVNAVAPGPILRPEAFSDAEDREVLKATPLGRWGGAEEV
                     ARAVLFLIETEFVTGETLRVDGGRHLN"
     gene            5958..6617
                     /locus_tag="VFD06_13935"
     CDS             5958..6617
                     /locus_tag="VFD06_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZN04679.1"
                     /translation="MSARTARPVRSIRPLAVFALTALVTAGVAAGEARAADTYSIDPS
                     HSNVGFTVRHFFSKVPGHFTKYEGTIVYDTQDLSKSTVTVSIDTASIDTEEPKRDAHL
                     KSPDFFDAEKHPKITFVSSKVTPAGDKKVKVDGTLTIRGVSKPVTLDVDLLGTGPDGW
                     GGVRSGFEARTKINRQDFGVAWNKVVEGGGSVLSDEVDIVLNVEAVRQAPKPAEAPKK
                     G"
     gene            complement(6683..>7245)
                     /locus_tag="VFD06_13940"
     CDS             complement(6683..>7245)
                     /locus_tag="VFD06_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04680.1"
                     /translation="AADPNGFSQIAFGLWNAHTTGLGRTIFPSDSYDLVEFDWFANVT
                     SFGGPFLSPSIFGGSVGGNAFDNFAFQSAEIALPLDVPLLCEGVYDAASRTLTVTVSR
                     HVRGPRFDPVPGAEATVDLSALDPGFLVDTFGIGAYGEGWPSLRAEVDFDLLYAGTAP
                     VPLSVVGAAPGPPRHVLVPVSLVPAP"
BASE COUNT         1142 a         2473 c         2622 g         1008 t
ORIGIN      
        1 agccgatccc ggcgctcaac accgcgctcg agccctacat cccggccatc aagacgttcc
       61 cgcaggcctg catcgagtgc cacaagttgg cgctgaacaa caacggcgcc gctctcggcg
      121 agagcttcct gccgttccgc gcctgcccgg agaaaccgca gcctgggcag cagctgccgg
      181 ccaactgcac gccggggaag gtgaacacgc tggcggcgca ccactgaggg cgcgctactc
      241 gccgatcact ttcaccagca cgcgcttcgg gcggcggccg tcgaactcgc cgtagaagat
      301 ctgctcccac ggcccgaaat ccagcttgcc cccggtgacg gccaccacca cctcgcgacc
      361 gaacagctgt cgcttgatgt gcgcgtcgcc attgtcctcg ccggtcaggt tgtgcctgta
      421 tgtgctggtc ggggcgtgcg gcgccagccg ctccaggaac tcctcgaagt cccggagcag
      481 ccccggctcg tcgtcgttga tgaacaccga cgcggtgatg tgcatggcgt tgacgaggca
      541 cagcccctcg ccgatgccgc tgtcgcgcag gcactcattg acttgcggtg tgatgttcag
      601 gtaggcccgc cgctccggga cgttgaacca gagctccttg cggtaggact tcatgcgcgc
      661 tcccgcccgg gtggcgcccg ggtccgggca gcataccgct tgatcgctcc ccggcgctcg
      721 ggtaggctct ccgcgaccat gacaacatcg accggaaccg aggatccgcg cgcgatccgc
      781 gaggcggcgc gcgctttgcg tccgttcatc gtcgacctga cgctgcggat ctgccgggtc
      841 cgtacggtga acttcatgcc ggacgacttt cccggcggcg ggccggacga catgccgggg
      901 ccggggatgg agtcgcgcgt cacctcgatc ctgggggagg agtgccgccg cctgaaaatc
      961 ccgttcacga cgcacgccaa ggtcgagggg cgggaaagcc tgctcgcgac cgtcggacag
     1021 aagcagcccg gctaccggca catgctcgtc ctgctgcaca ccgacacggt gccggccggc
     1081 tcaccgaccg tctggaagcg gccgccgttc gagccggtgg aagagggcgg gacgctctac
     1141 ggccgcggtg tgctcgacga caaggggccg ctggcggcgt cgttcgcggc gttgcggctc
     1201 ctcaaggagc gcgaggatcg catcccgggg gctttcacct tcggcgcggt ggcggacgaa
     1261 gaggtgggga tcggtgtcgg gctcccattt ctcatcgaac aggggctcat cacctgcacc
     1321 gacgccgtca tcccggacat cgccggcaac atgaaggaga tcaacatcgc cgagaagggg
     1381 cgagcgctgc tgcgcatcgc gacgcgcggg aagcaggcgc acgccatgga gccggccaag
     1441 ggggtcaacg ccatcctggc cctggcgcgg gcgctcctgg cggtggagcg gatgacgctc
     1501 gagcatcggc cgcacccgct gctcggcggg ccgacgatca acccgggcct ggcgcgcggt
     1561 ggcgtggcgc cgaacgccgt gccggccgac gcgcaggcga tcctcgacat ccgctacgtc
     1621 ccgggacaga cggcggaggg gatccggagc gagattgccg cggccgcatc gaaggcgatc
     1681 gccgaccttc ccggagccgc ggtcgaggtg gagatcgtcc aggaggcgaa gccgtgcgag
     1741 gtctcgcccg acgcaccgat cgtgcgccga ctgctgcgcc acgcgccgga ggcgaaggtg
     1801 atcggctcgg gaggaggaac tttcgccaag gacctcgtcc tcatgggggt cgatgcggtg
     1861 ggatggtcgg cgggcgacga ggagacctac caccagccga acgaggagat cgacgtggag
     1921 cagctggtca ccttcgccgg ccgcctcgcc gccctggcgc tcgagattgg atccgagaag
     1981 gtcgagggct cgtgaggtcg gtgatcgcgc tcggcctcgg cgtcctcctc ctggtcgggc
     2041 tcgtcttcgt tctgctcgcg tgcgcgcgcg gcgaggccgg cgcgccgggg cagccggccc
     2101 gcagggcaga ggtcccggcg gcggcgccca cggacgccgc ggcggggcac cacgcgcatg
     2161 gggcggcggc cgggcccgat gccgctgccg atcacgcgac cgtccatcac gccttcaacg
     2221 acccggagcg atgggcgaag gtgttcgacg atccggcgcg cgacgcgtgg cagaagccgg
     2281 tcgaggtggt ggcgtccctc ggcctcagaa aaggaatgac ggtcgccgat ctcggcgccg
     2341 gcaccggcta cttcaccgcg gcgctggcgc gcgccgtcgg cccgactggt gtggtcctga
     2401 gcatcgaccc ggaacccgag atggtggagt atctcggacg acgggcgcat cgcgaggggc
     2461 tcgccaacgt cgtcccggtc ctggcgctgt acgaggaccc cttcctgcct ccggggcggg
     2521 tcgatcgcgt cctcatcgtg gacacgtacc accacatcga cgaccggcag cagtacttcc
     2581 gccgcgtcgg cgacgacctc gcccccggtg ggcgcgtggc ggtcgtcgac ttccacaagc
     2641 ggccgctgcc ggtcgggcct ccgcccgaag ccaagctgga tcgcgaggtg gtggtggcgg
     2701 agatgcagaa ggcggggtat cggctcgctg aggagcccac cttccttccc tatcagtact
     2761 acctggtgtt cgagccgatt cgaggagccc cccagtgatc gtcacctccg acttcaagcg
     2821 cggcctcgcc atcctggtgg aggggaagcc gttcatcatc accgagtaca gcgtgcagac
     2881 gccgtcggca cgcggcgccg ccaccctcgt gcgcgtcaag gcgcgcaacg tcctcaccgg
     2941 ccaggtgctc gacatgacct tcaagtcggg ggacaagttc gacgagccgg acctggaata
     3001 ccgcaagacg tcgttcctgt acgccgacgg cgacgagtac cacttcatgg acgagacgtc
     3061 gtacgagcag ttccatctgt cgaaggagac gatcggcgac gacgcgcgct ggctgaagga
     3121 cggcgtcagc gtccgggccg tcaccttcga cggccgggtg tacgggatcg agctgccgca
     3181 gttcgtcgag ttcgaggtga tcgagaccgg tccggggggc cgttccgaca tggcgagcgg
     3241 caaggtgatg aagcctgcga cgttgtcgaa cgggacgcag atccgcgtgc cggtctacct
     3301 cgacgcggga gaaaaggtga tggtggacac gacgaccggc gagttcgtcc ggcgcgtgtc
     3361 ggaggagaaa cgatgaagat ctcgcgcagg gtcgtgggac ggatgatcct cgggacgccg
     3421 gctctgctgg ccggggcggg gctcatgggg acgctcggac agggggcgcg cgccgacgag
     3481 ccgcaggcgt cacccgcgcc ggagccggag gccgagccga cccctctcgc gaagttcatc
     3541 gcgaagcagg aggaggggtt gagcggcgcg gagcgggaca aggtccgcaa ggacgtgacc
     3601 cagctcgagg aggcgctcaa ggtggtgcgc gacttcccga tcgccaacga catcccgccc
     3661 gccggcggct tccggccgat gcgctcggtc cggaagggcc gctgaggagg agccgatgcc
     3721 gacgccgaac ctctccgagg agacgctctt cctgccggtg accgatctcg cggcacgcct
     3781 gcactcgcgc cggctctcgc cggtcgccct gaccgaggcg tatctcgccc gcatcgacga
     3841 gctcgacccg gcgtaccacg cctgggtgac ggtgatgcgc gacagcgccc tcgcggaggc
     3901 gaagcaggcg gagcgcgaga tcgcccgcgg ccggatccgc ggcccgcttc acggcatccc
     3961 gtacggcgcc aaggacctcg tcgcgacgaa ggggacccgc accacctggg gggcgaagcc
     4021 gtacgagcag cagtcgttcg actacgacgc ggcggtcatc cgcaagctgc gcggcgccgg
     4081 ggcgatcctc ctcggcaagc tggccatgat cgagatggcc ggcgggctcg gctacgagaa
     4141 agggacggcg tccctcaccg gagcggcgcg caacccctgg gacccgtccc gctggacctg
     4201 cgggtcgtcg tccggctccg gggcggcggt cgggaccggg atgctgccgt tcgccatcgg
     4261 ctccgagaca tgggggtcga tcgtctgccc gtccgcgttc tgcggcatca ccgggctgcg
     4321 cccgaccttc ggccggatct cgcggcaggg ggcgatggcg ctgtcgtgga ccctcgacaa
     4381 gctggggccg atgggacgga ccgcgcgcga ctgcgaggtc gtctatcgcg tgctctcggg
     4441 acgggatccg gacgacccgt acacgacgga cgagccgctc gggccggagt cttcgtcggc
     4501 cggggcgcag cggctcaagg tcggcttcct gcgcctggac ttcaagaaga gcggcaaccc
     4561 ctcggtggag cacgcgttca tgaaagcgct cgacgatctc cggggggcgg gagtggcggc
     4621 ggaagagacg cggctccccg agctgccgtt cgaggcggcg accgcggtga tcctgacctc
     4681 cgaggtcgcg accgcctacg aggacctcca acgcggcgac aaggtgcggc agctctcgag
     4741 tccgggcgcc gcgctggcct tcgtcaccgc ccacgcggtg cgcggctccg actacgtcaa
     4801 agcggagcgc ctccgaaccg tctgcgtccg ggcgatggcg gatctcttct cgaagtacga
     4861 cgtcatcctg tacccgaccg agatgcacac cgccttcggc tacgatcagg acttctcggg
     4921 tgtgtcctgg tccgacccgg ccggcggcgc cggcaacctg tgcggcctgc cggcggtgtc
     4981 ggtgccgtgc ggcttcgccg acgacgggct gcccgcgggg ctcggcgtcc tggccggagc
     5041 ttacgacgag gcgaaggcgg tgacgctggc gcggcattac cagtcgatca cctcctggca
     5101 cacgcgcagg ccccccgtga cgagcgcgcg gacctgaagg gcgcgggacg gagcgggagc
     5161 gcggaaatgg atccggccgg gaaagtggcc ctgctcaccg ggacggcgcg catcggccgg
     5221 accgtggccg agaccctggc gcggcgcggg gcggacgtgg cgatctcctg gcgcacctcg
     5281 cgcgacagcg ccgaggagac cgccgaccgg gtgagggccg ccggccggcg tgccgtcacg
     5341 atccaggccg acctgagcga tccgcaggcg gcgcgggcga tcgtggacga ggtcggccgc
     5401 gatctcggag gccccgacat cctggtgccg atggcgtccc attacgagaa ggtgccgttc
     5461 gacctcctcg acgacgccgc ctggcgcagg aacgtcgacg ccgacctcca gagcacctac
     5521 cggctggcgc ttctggcggc gcggccgatg cgggaacggg ggggcgggcg catcgtcgcc
     5581 ttcgccgact ggttgccccg gagcggacgg ccgcggtata agggcttcct tccgtactac
     5641 gtggccaagg ccggggtcat cggcctgatc gaggcgctgg cgctcgagct ggcgccatcg
     5701 atcctggtga acgcggtggc accggggccg atcctccgac cggaggcgtt cagcgatgcg
     5761 gaggaccgcg aggtgctgaa ggcgacgccg ctcgggcgct ggggaggagc ggaggaggtc
     5821 gcgcgtgccg tgctgttcct gatcgagacc gaattcgtca ccggcgagac cctgcgcgtc
     5881 gacggtggcc gccatctgaa ttgaaccgca accgcaaccg caacgtggcg ttcgtcgcac
     5941 acccgaggag ggaacgcatg tccgcacgca ccgctcgtcc cgtccgctcc atccggccgc
     6001 tcgccgtctt cgccctgacc gccctggtca cggcgggtgt ggcggcggga gaggcgcggg
     6061 ccgccgacac ttacagcatc gacccgtcgc actcgaacgt cggcttcacc gtccggcatt
     6121 tcttcagcaa agtgccgggg cacttcacca agtacgaggg gacgatcgtc tacgacaccc
     6181 aggacctctc caagtccacg gtgaccgtca gcatcgacac cgccagcatc gacaccgagg
     6241 agccgaagcg cgacgcccat ctcaagagcc ccgacttctt cgacgccgaa aagcacccga
     6301 aaatcacctt cgtttccagc aaggtgacgc cggccggcga caagaaggtc aaggtggacg
     6361 ggacgctgac gatccgcggc gtcagcaagc cggtgacgct cgacgtcgat ctcctcggca
     6421 cggggcccga cggctggggg ggcgtccgat ccggcttcga ggcgcgcacc aagatcaacc
     6481 gccaggactt cggcgtcgcc tggaacaagg tggtcgaggg gggcggctcg gtgctgagcg
     6541 acgaggtgga catcgtgctc aacgtcgaag cggtccgcca ggcccccaag ccggccgagg
     6601 cgccgaagaa gggataagcg agcgccgttc tcgttttcgc cccctccggc cgcgcgccgg
     6661 agggggctct ttcttgccgg gctcaggggg ccgggacgag ggagacgggg acgaggacgt
     6721 ggcgcggcgg ccccggcgcg gccccgacga ccgacagcgg gaccggcgcg gttccggcgt
     6781 agagcaggtc gaagtccacc tcggcccgca gcgatggcca tccctcgccg tacgcgccga
     6841 tgccgaacgt gtccaccagg aagccgggat cgagggccga gagatcgacc gtcgcctcgg
     6901 cccccggtac cggatcgaag cgcgggccgc ggacgtggcg cgacacggtg accgtgagcg
     6961 tccgcgacgc cgcgtcgtac accccttcgc acaggagcgg cacgtccagc ggcagggcga
     7021 tctcggccga ctggaacgcg aagttgtcga aggcgttgcc gccgacgctc cctccgaaga
     7081 tcgacgggct caggaacggc cccccgaacg aggtcacgtt ggcaaaccag tcgaactcga
     7141 cgagatcgta cgagtcggac gggaagatcg tccggccgag gccggtggtg tgagcgttcc
     7201 acagtccgaa ggcgatctgc gagaagccgt tcgggtcggc agcga
//
LOCUS       DASRIQ010000563         6357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1534442, whole genome shotgun sequence.
ACCESSION   DASRIQ010000563 DASRIQ010000000
VERSION     DASRIQ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534442"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFD06_13945"
     CDS             <1..318
                     /locus_tag="VFD06_13945"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZN04681.1"
                     /translation="EAGLDLDRRRFRANIVVETERGEPFLEDEWVGRTLMFGDGDPRP
                     AVNVTMCDLRCVMINLDPETGAQDARIMKTVVRLNKNYAGVYGTVARSGPIRVGDPVN
                     LVS"
     gene            335..742
                     /locus_tag="VFD06_13950"
     CDS             335..742
                     /locus_tag="VFD06_13950"
                     /inference="COORDINATES: protein motif:HMM:NF022164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaF N-terminal domain-containing protein"
                     /protein_id="HZN04682.1"
                     /translation="MKAILRRLLLALLVTASALFEPGLIAADPAPGEVRMRHVGRSFV
                     FDYGEMVIRVRYLSDSRLEWEQTKGPQAGLKAEEQYGSAAVRDDVVFFWWQEKDQSVV
                     SQVVDFGKGVVYTTWTSPDRKLAQFQGKVRPQQ"
     gene            739..1407
                     /locus_tag="VFD06_13955"
     CDS             739..1407
                     /locus_tag="VFD06_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04683.1"
                     /translation="MKPRWIGSLTLAGGIITLALAPLLVAIKYMTGWAVVPEPFWVPG
                     VRGWLAEAFPAATPAVLWMAFGSGYSIALLLLLGGFVALSPLLEGRTGIQRSGYWMVA
                     GGLALVLPGDAIHSWTWHQNGLTVPTPGTNPTANTAYAAHMMGMNFIMLGTLLLGSSA
                     LRHGTLARWLAWLFALVFPAALLASLTVLPTTPSGALWLFSVAMGLLGWQVARGRLRW
                     PQYS"
     gene            1404..2351
                     /locus_tag="VFD06_13960"
     CDS             1404..2351
                     /locus_tag="VFD06_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04684.1"
                     /translation="MSRGAGVVRVLLFCAAVWAGALTVVSLHRAAEGAAGPELFGEGV
                     VSTGDFESHPAFTPDGRMLYFVKSTPAFTDWKIYVTQMEDGRWAPPKVAPFSGKHRDA
                     DPFVTPDGKRLYFISDRSSDGRPRENMDIWVMERNRNGSWGEPRNLGAPVNTPASEWL
                     PRPASSGTLYFGSDRPGGLGKTDLYRARSVGGRFSAPENLGPAINSAADEYEGAIAPD
                     ESFIVFMAAGRPDDRGGGDLYISHRKDGAWTQAKNLGATINGPGLEIAPYFTPDGKSF
                     FFSSARKAPGTPPGQRPNRARNGLGDIYRIDLQALHELP"
     gene            2348..2905
                     /locus_tag="VFD06_13965"
     CDS             2348..2905
                     /locus_tag="VFD06_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN04685.1"
                     /translation="MTAPLKASVFVGTSLDGYIARPNGDLDFLPSDGGEPHGYDEFMA
                     TVDALVIGRKTYETVLSFGAWAYGAKPVFVLSTRPIASAPPGAIVEGLSGPPDDIASR
                     LAARGIGHIYVDGGDTIQRFLRAGLIQRLVITRVPVLIGSGIPLFGPLPKDIRLRHVA
                     TRSYVSGLVQSEYAVEAGPLPESTR"
     gene            3049..3174
                     /locus_tag="VFD06_13970"
     CDS             3049..3174
                     /locus_tag="VFD06_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04686.1"
                     /translation="MDGKPVAERGGNWFTRPFTSYPDFDAIVEQIDRQRPGKPAG"
     gene            complement(3171..4196)
                     /locus_tag="VFD06_13975"
     CDS             complement(3171..4196)
                     /locus_tag="VFD06_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HZN04687.1"
                     /translation="MRSSAAVAPIDRWAAWDRFLESTPATGFMQSSWWADFRTTVGFS
                     HFAAILKGPGGIVGGGMVQRLRHRSGAVFYYMQDGPVLPTDAALAGDVLEATLATLEE
                     RRATEKENVTHLRIEPRWEEGAGIPLAGFEPVARRDRYVEPRNTLCVDLRPKPDGILA
                     GMRPKGRYNISVARRHGVSVVEDTSPRGLADFLRIYRDTAGRQGFRTKPKSYFDALLA
                     VAAPRGRGALLFAEHQGRRLAAALVILFGSRATYFYGGSLAERRHIMAPYLLHFVAMC
                     RAKVAGCEWYDLWGVAPEGEPAHPWAGISVLKRRFGGREVRLVPTRDYVYDRSAYMRY
                     QAAEVAR"
     gene            complement(4276..5265)
                     /locus_tag="VFD06_13980"
     CDS             complement(4276..5265)
                     /locus_tag="VFD06_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04688.1"
                     /translation="MIGIRSIEDAPAACESAAVKKARAAARTHAKHETLFLTGRPTLK
                     HFIRFARTNAVKPRSAGTLADEWRGAYGLVRDLEASEAGVADDPPIVRVSVEEHAPLL
                     AEFLKNPLVRHGFNIVPSEVAFVELDRLVVSQKHIDLVHVRRLVASLGRNPTPERVFR
                     ACLASDQPRPQVTWASEGEGRYVFVSPSNDLRYLGVMSMKERHIRGLAPPGDLVGVVG
                     MAVGFGSNFLSVIHAENRLILHNGSHRAYALRTIGLKSVPCIVQHVSSRDELEVVASP
                     EVRRHPDRYLKDPRPPMFADYFRPGLQTILKVRPRLRQITVRVNVDESYVPAL"
     gene            complement(5309..>6357)
                     /locus_tag="VFD06_13985"
     CDS             complement(5309..>6357)
                     /locus_tag="VFD06_13985"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN04689.1"
                     /translation="LGAGVLAAVLFMTRLELRIAGPFNVLPEENADVRSPLEQIIEEI
                     YVDEGQIVKAGDRIARLDDRPLRAELARTDAAIEEGRAILRKLQVGATTAEIAVAKAG
                     VAKGEDSLMYATRRVEKLAILYQQGLVSQKELEDAQEVASSAANNRTEAGSRLRLLEV
                     GTRPEEIAATRARLEQLESQRRYLEEQRALLDIVSPVPGIVATPTRQLKELRRQLVKK
                     GDLILKVYDVSTVTAQIFVSERELDGIEVGQQVELRARAYPGTTFQGTVTSIATSAQG
                     SAGVTTGPGVFASGSVDPNKSVIVTTRIDNQSLLLKPEMTGQAKIFCGPRRIADLMAR
                     RVALTFRVALWSWW"
BASE COUNT          979 a         2205 c         2162 g         1011 t
ORIGIN      
        1 gaggccgggc tcgatctcga ccgccggcgc ttccgagcca acatcgtggt cgagacagag
       61 cgcggcgagc cgttcctgga ggacgaatgg gtcggccgaa cgttgatgtt cggggacggc
      121 gacccgcgtc cggcggtgaa cgtcacgatg tgcgatctgc gctgcgtgat gatcaacctc
      181 gacccggaga cgggagcgca ggacgcgcgt atcatgaaaa cggtcgtgcg gctcaacaag
      241 aactacgcgg gtgtgtacgg gacggtggcg cgaagcggcc cgatccgtgt cggcgatccc
      301 gtgaaccttg tgtcgtgatg gcggaggggg ggacatgaag gcgatccttc ggcggctgct
      361 cctggcgctc ctggtcacgg cctccgctct cttcgagccg ggcctcatcg cggccgatcc
      421 ggctcctggc gaggtgcgca tgcgtcatgt cggtcgttcg ttcgtgttcg attacgggga
      481 gatggtgatt cgagtccggt atctctccga ttccaggctc gagtgggagc agacgaaggg
      541 gccgcaggcg gggctgaagg cggaagagca gtacggatcc gcggccgtcc gggacgacgt
      601 cgtcttcttc tggtggcagg agaaggacca gtcggtcgtg agccaggtcg tcgatttcgg
      661 gaagggagtc gtctacacga catggacctc tcccgaccgg aagctcgcac agttccaggg
      721 caaggtcagg ccgcagcaat gaagccgcgc tggatcgggt ccctcacgct ggccggcggc
      781 atcatcaccc tggcgctcgc tcccctgctg gtcgccatca agtacatgac cggctgggcg
      841 gtcgttcccg agcccttctg ggtccccggc gtcagaggct ggctcgccga agcctttccg
      901 gccgcgacgc cggccgtgct gtggatggcg ttcggcagcg gctattccat cgcgctcctc
      961 ctgctgctcg ggggattcgt cgccctgtcc ccgctcctcg agggacggac cggcatccag
     1021 cggtccggct actggatggt ggccggaggc ctggccctgg tgctgccagg cgacgccatc
     1081 cattcctgga cctggcatca gaacgggctg accgtgccga cccccgggac caacccgacg
     1141 gcgaataccg cgtatgcggc ccacatgatg gggatgaact tcatcatgct cgggacgctg
     1201 cttctggggt cgagcgccct ccgtcacggc acactggcgc gctggctcgc gtggctgttc
     1261 gctctcgtgt tccccgcggc gcttctcgcc tccctgaccg tcctccccac cacgccgagc
     1321 ggcgccctct ggctcttcag cgtcgcgatg ggcctgctcg gctggcaggt cgcgcgcggc
     1381 cgtctgcgat ggcctcagta ttcgtgagcc gaggagccgg agtcgtccgc gtcctcctgt
     1441 tctgcgcggc cgtctgggcc ggcgccctga cggtcgtttc gttgcaccgc gccgccgagg
     1501 gcgccgccgg accggaactg ttcggcgagg gggtggtctc gaccggggac ttcgaatccc
     1561 accccgcctt cactccggac ggcaggatgc tctatttcgt gaagagcacg cccgcgttca
     1621 ccgactggaa gatttacgtc acgcagatgg aggacggccg gtgggcaccg cccaaagttg
     1681 ctccgttctc agggaagcat cgcgatgccg acccgttcgt aacaccggac ggcaagcggc
     1741 tctacttcat ctcggatcgt tcgtcggatg gacggcccag ggagaacatg gacatctggg
     1801 tgatggagcg caaccgcaat gggagctggg gtgagccgcg caacctcgga gccccggtga
     1861 acacgcccgc cagcgagtgg ctgccgaggc cggcgtccag cgggaccctg tacttcggat
     1921 ccgatcgtcc cggcggcctg gggaagaccg acctgtatcg cgccaggagc gtcggcggaa
     1981 ggttcagcgc gcccgagaac ctggggccgg cgatcaacag cgccgcggac gagtacgaag
     2041 gagccatcgc ccccgacgag agcttcatcg tgttcatggc cgccggccgg ccggacgatc
     2101 gcggcggcgg ggatctgtac atcagccatc gcaaggatgg cgcgtggacc caggcgaaga
     2161 acctcggcgc gaccatcaac ggtccgggcc tcgagatcgc gccgtacttc acgcctgacg
     2221 gtaaatcttt cttcttctcc agcgcccgca aggcccccgg cactcctccc ggccagcggc
     2281 cgaaccgggc gcgcaacggg ctcggagaca tctatcgcat cgacctccag gctttgcacg
     2341 aactgccatg accgcgccgc tgaaagcatc cgtgttcgtc gggacgagcc tcgacggata
     2401 catcgcgcgc cccaacggcg atctcgactt ccttccttcg gacggcgggg agccgcacgg
     2461 ctacgacgag ttcatggcga cggtggacgc gctggtgatc gggcgcaaga cgtacgaaac
     2521 agtgctgtcc ttcggcgcgt gggcctatgg cgccaagccg gtcttcgtgc tcagcacgcg
     2581 gccgatcgcg tcggcgccac ccggcgcgat cgtcgagggc ctgtccggtc ccccggacga
     2641 catcgcctct cgtctcgcgg cgcgcggcat cggccacatc tacgtggacg gcggagacac
     2701 catccagagg ttcctgcggg cggggctgat ccagaggctc gtcatcaccc gcgtcccggt
     2761 gctgatcggg tcgggaatcc cgctcttcgg ccccctcccc aaagacatcc ggctcaggca
     2821 cgtcgcgacg cgctcgtacg tgagcggcct cgttcaaagc gagtatgctg tcgaagccgg
     2881 accgttgccc gagtctacga ggtgaacccg atgaaaatcg agatcgtgca ctgcccgacg
     2941 tgacccaata accgcgccaa ggccgcgagg ctcgcggccc tcatcaggca gcacctcggt
     3001 ctcgaggccg agaagacgtc gggcggcatc ggcgagttca aggtcctcgt ggacgggaag
     3061 ccggtcgccg agcggggcgg caactggttc acccggccct tcaccagcta cccggatttc
     3121 gacgccatcg tcgagcagat cgaccggcaa cgcccgggca agccggccgg ttagcgggcg
     3181 acctccgccg cctggtagcg catgtaggcg gagcggtcgt agacgtagtc gcgcgtcggc
     3241 acgagccgca cctcgcgtcc gccgaaccgg cgcttcagca cgctgatgcc ggcccaggga
     3301 tgcgccggct ccccttcggg agcgaccccc cagaggtcgt accactcgca ccccgcgact
     3361 ttcgcccggc acatcgccac gaagtggagc aggtagggcg ccatgatgtg gcggcgctcg
     3421 gccagcgacc ctccgtagaa ataggtcgct cgcgagccga acaggatcac cagggccgcg
     3481 gccagtcgcc gtccctggtg ctccgcgaat aggagcgcgc cgcgccctcg gggcgccgcc
     3541 acggcgagca gagcatcgaa gtaggacttc ggcttcgtcc ggaagccctg acgcccggcc
     3601 gtgtccctgt agatgcgcag gaagtccgcc agacctcgcg gcgacgtgtc ctcgacgacc
     3661 gacacgccgt gccgccgggc gaccgagatg ttgtaccgcc ccttcggcct catcccggcg
     3721 aggatgccgt caggcttggg gcgcaggtcg acgcacagcg tgttcctcgg ctccacgtag
     3781 cggtcccggc gggcgaccgg ctcgaagccc gcaagcggga tccccgcccc ctcctcccat
     3841 cgcggctcga tgcggagatg ggtgacgttt tccttctccg tggcacggcg ctcctcgagc
     3901 gtcgccaggg tcgcctcgag cacgtctccg gcgagcgccg cgtccgtcgg gagcaccggc
     3961 ccatcctgca tgtaatagaa gacggccccg gaccgatgac ggagccgctg caccatcccc
     4021 ccgcccacga tgcccccggg gcccttcagg atcgccgcga agtgggagaa accgacggtg
     4081 gtgcggaagt ccgcccacca ggacgactgc atgaagccgg tcgccggcgt ggactccaga
     4141 aaccgatccc aggccgccca gcgatcgatc ggcgccacgg ccgcggagga acgcatggcg
     4201 cccgccatgg cggccgggat cccggcgaac ggggcgtcgc gcaaaccccg acgactcgcc
     4261 ggctgcgtcg cactctcaga gcgcggggac atacgattca tccacgttca cccggacggt
     4321 gatctggcgg aggcgcggcc gcaccttcag gatcgtctgg aggccggggc ggaagtaatc
     4381 cgcgaacatc ggcgggcgcg ggtccttcag gtagcgatcg ggatggcgcc gcacctccgg
     4441 cgaggcgacc acctcgagct cgtcccggct ggagacgtgc tggacgatgc acggcacgct
     4501 cttgaggccg atcgtccgca gagcgtaggc gcgatggctg ccgttgtgca ggatgagccg
     4561 gttctcggcg tggatgacgc tcaggaagtt cgaaccgaat ccgaccgcca tcccgacgac
     4621 gccgacgagg tcccccggcg gcgcgaggcc gcggatgtgc cgctccttca tcgacatgac
     4681 gccgaggtag cgcaggtcgt tcgaagggga cacgaagaca taccggccct ccccctccga
     4741 ggcccacgtc acctgaggtc gcggctgatc ggacgccagg caggcgcgga agacccgctc
     4801 gggggtcggg ttcctgccca ggctcgccac cagccgtcgg acgtgcacga gatcgatgtg
     4861 cttctgcgac acgacgagcc gatccagctc gacgaacgcg acctcgctcg gcacgatgtt
     4921 gaatccatgg cgcaccaggg ggttcttcag gaactccgcc aggagcggcg cgtgctcctc
     4981 caccgagacg cggacgatcg gcgggtcgtc ggcgacaccg gcctccgatg cctccagatc
     5041 gcggaccaga ccgtaggcgc ccctccactc gtcggcgagc gtccccgcgc tgcgcggctt
     5101 gacggcattc gttctggcga agcgaatgaa gtgcttgagc gtcggccgcc cggtgagaaa
     5161 gagggtctcg tgcttcgcgt gagttctggc ggccgcccgc gctttcttca cggcggccga
     5221 ctcgcaggcg gccggcgcgt cctcgatgga gcgtatcccg atcatccgtc cctcctcgga
     5281 ggccgccgcg tggcgccccc tgacaggctt accaccacga ccagagagcg acccggaagg
     5341 tcagcgcgac gcggcgcgcc atgaggtcgg cgatccgccg cgggccgcag aagatcttcg
     5401 cctgcccggt catctccggc ttgagcagca gcgactggtt gtcgatccgc gtcgtgacga
     5461 tgaccgactt gttcgggtcg acgcttccgg aggcgaagac gccggggccg gtcgtcacgc
     5521 cggcgcttcc ctgggccgac gtggcgatgg acgtcaccgt gccctggaag gtcgtccccg
     5581 gataggcgcg ggcccgcaat tcgacctgtt gcccgacctc gatgccgtcc agctcccgct
     5641 ccgaaacgaa aatctgcgcc gtgaccgtgg agacgtcgta caccttgagg atgaggtccc
     5701 ccttcttcac gagctggcgg cgcagctcct tgagctgccg ggtcggggtg gcgacgatgc
     5761 ccggcaccgg gctgacgatg tccaggagcg cccgctgctc ctcgaggtag cgccgctgcg
     5821 actccagctg ttcgagtcgc gcccgggtcg cggcgatctc ctccggtcgg gtccccacct
     5881 cgagaagtcg cagccggctt ccggcctccg tccggttgtt ggccgctgaa cttgcgactt
     5941 cctgtgcgtc ctccagctcc ttctgggaca cgagcccctg ctggtacagg atcgcgagct
     6001 tctcgacccg tcgcgtggcg tacatcaggc tgtcctcgcc tttggccacg ccggccttcg
     6061 ccacggcgat ctccgcggtg gtcgccccga cctggagctt gcgaaggatg gctcgcccct
     6121 cttcgatggc ggcgtccgtg cgggccagct cggcgcgcag cggccggtcg tcgagccggg
     6181 cgatccgatc gccggccttc acgatctgcc cctcgtcgac gtagatctcc tcgatgatct
     6241 gctcgagggg agagcgcacg tccgcgttct cctccgggag gacgttgaaa gggcccgcga
     6301 tccgcagctc gagccgggtc atgaacagga cggccgccag aacgccggcg ccgaggg
//
LOCUS       DASRIQ010000564         7845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_465274, whole genome shotgun sequence.
ACCESSION   DASRIQ010000564 DASRIQ010000000
VERSION     DASRIQ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465274"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFD06_13990"
     CDS             complement(<1..495)
                     /locus_tag="VFD06_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04690.1"
                     /translation="MTRRARRPVYWLSLLVLLPGALLFGGRPQPLEDSGPDDPVERAM
                     QAAQARVLDRLGPVEAAMPPPEFSPLGGPLLVQVLQGEAAKGMLLTSQGHVDWDADKD
                     AVAAKIPAALRLSAGEVRRFGHGTLRPGLNYLRLSEAEVSRRGLDAVQSEVARHARIL
                     GWLPE"
     gene            complement(787..7770)
                     /locus_tag="VFD06_13995"
     CDS             complement(787..7770)
                     /locus_tag="VFD06_13995"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN04691.1"
                     /translation="MEKHEKRNNTEAVHLSADTNIDFVFNESVLYTGLWRVTVKRGLG
                     GAIPGSITITAPTVAEDDDQNEDDNNNGRLDAGEDDNANGLLDQLGQPYALVVSGPVF
                     LAEAAPALGAQTFPQSRVDFDRIAYDCGKDAVATILDTTPAASAALSSSATTFTVVAA
                     DGTVLDTETGLSFTLLGPGKTASAPIPVRLAGPALSGNGILEGDTGQRVRARYAPPGQ
                     PAVEAEASVRCSPDLVFTPFLTAGGNAVGEQVSIQGGCDQDAFLDAGEVVSYGVALRN
                     RSRDAGYADAVATLTPSGPGAAAVRVLDSPRHLGILPAQGVNAVFFQVYVDPTVVNAL
                     SVANRVVTMRLDLDSSSRGTRLARQSYSFTHALNSDRETFFYSTDFPNGGRQVRDINR
                     NFVVDPPDTTDPLLGFIVAREDVTFASLFSGTGAPLGHFTNEIGEDFDLSGTFSGTER
                     NVVPNVDPDGNPVLDRGILNSNNPADPNHRVPWNFDNNSGGWAAFRHPGSTAAGVNVN
                     PLWEYKTSGLCGFQTSGGANKFGIWHTGDGDPTTPSGVATPCDNHTQPHDPNTPAKVE
                     LLMDVLESPIVAKVNQNNDARGFSYTVEFQRFAYNQNIQINDGYAGGGVNIDNDIDSD
                     NANSLLAQQMDQYYTRRTGGWPYGMFRDAGQYFPGDGIDPTTTIPFQRTFGPFTNPNA
                     SVNLDGDETGFSGATQNTNPDSSTPIPTAIPDFLPYPVPGAPVIGVCDGGPQNDATCD
                     PGNAGDPCITGGGACFAAVNTVNGPVRNWDATLIGYEGGFASLIDDGPHENFFFFLPG
                     KAGNRWQIGIGFWAMEGTTGTTDYGKSIDDAVFEWKEYHPQDEAALGAQPACARFNGI
                     GQPAGGQCATVTVDRTSLYECEETVEITVRDAKCRTIGAGNTVPLAGACTTNAQCGSG
                     GTCTAALASVEVAVATDSDGTSGGPLILTPAAKHFVLPAVPGQPGLFRGRVSFTTLPA
                     DATRVHVSAGSDSGFVVEYFDPLCDGDRDGQAGESAFDNIDGDGIPDAADHCPGVYDP
                     LQQDADADGLGDLCDNCPVVANTPQADTNADGVGNACEFDDIDGDGLPNGADTCPDVR
                     NPNQSDIDADGRGDLCDTLKTSGVIFGTSAGAADCVSGLCTRPGDAIGDACVTDENCI
                     RSCFNNVCTQGNLGAAGGLRCIGGSNNGNVCTVAADCAAPGVCSANFVSPTPKIGQAC
                     STHAQCYADVDRDADGVIDSNDNCVIAPNGPLLGPNNQLDSDQDGLGNACDGDCAVAA
                     VAYRCRANGAACSVPETSQAACANSFGLGDVCQWYLASSGGCSSVDDDGDADGVQDLA
                     DSCAMTANPRVVAGGAQPDRDRDGLGDACDPAGAFDDAGDGVPDDVVTFQGTVACRTQ
                     PLVNVAILGATYLDLDGDHDPYPDTGETGRVSLQIRNLGPALQDAVFTLTSSDPDVAC
                     ITETQVRVASLPNNATVTLGSLDPGQPGFTFVASNALQAMPPPAPFPTINLGLQITAA
                     GALGTSAPIAFSLLADIDSPFGPQQFTLGPDGIAGTSDDGTVVEKFDLDRDGDGNFTV
                     KDTFLQPIAPGVYRGTCSNAPLTNCITAADCPANPPGAVCYSGAYLRGSDSGAGLNRV
                     AAVTCGGFDTAPGTPLCALDPDFPMDWHLHCPPGAINCPNLESGSCVGGCSYNTPTDG
                     THSLSPPNSLHMGAHFLSTTAGDATHLRTLQGFVTPPVNLAMQPRPGDLDFSFFHIAR
                     LIDNNTGFTVAHTCQDCGDVQIQADLEGDPGVDVWGFWDKLVPYQNVYEHKPMSFSEF
                     GSYYCVFTPTDTGTAPPNPRGVHETICYPQGAWSRCGSTTGTSSISSGDCTGPGVVEP
                     SGLGVWVQTRFHLDPYIGQRIRIRWIAETWNFGGDFGSYYEIGGTWANLTGDDGWWLD
                     DIVLTGAVTSQVSLLRDNTPRTGTCPGDPCDAAVGDMGTAVVLTVTDAAGVAIDGVTR
                     IPATGSPIRVGAAGSSFPGGCSNGYAEYQFLRDGMVVQPWSGNAFFNDSPERTTRYTA
                     LVRCTSDPGCSSAAGASIDLPVRSGEGGDVVFGARGGSFDPGAGVVYYRGACSAGTVG
                     APCNSAADCGAGGACAVTATPADDVTVLRLWAASDDGLDVVKGTVPASPAPKGTLSGS
                     FWTLPGLSGPCFLSNLAPTSAATGFNHTSGPLTQAQDPNPAVGAVLYYQASANSPGGA
                     NLDAYGCANPAICSNPGWCEVGTNAGAPCTTNANCPGGTCVASATFCSTDAGSAGQGG
                     CGHHSTCAGGSSPGKLCQSVVDCPGGGTCPAPTTAQQAAEGQVCLTLTGAPLAPAPYG
                     NCPPAGHPKRVVSRAGAPPCP"
BASE COUNT         1145 a         2637 c         2828 g         1235 t
ORIGIN      
        1 ctccggcagc cacccgagga tgcgggcgtg ccgggccacc tccgactgca ccgcgtctag
       61 cccgcgacgc gacacctcgg cctccgacag gcgcagatag ttcaggcccg gacggagcgt
      121 cccgtgaccg aagcgccgca cctcgccggc cgacagccgg agcgccgcgg ggatcttcgc
      181 ggcgacggcg tccttgtccg cgtcccagtc cacgtgcccc tggctggtca ggagcatccc
      241 cttcgccgcc tcgccctgga ggacctgcac gaggagcggc ccgccgagtg gtgagaactc
      301 gggcggcggc atcgccgcct ccaccggccc gaggcgatcc aggacgcgcg cctgcgccgc
      361 ctgcatggct cgttcgaccg ggtcgtccgg gccggagtcc tcgagaggct ggggccggcc
      421 gccgaacagc agggcgcccg gaagcagaac gaggagggaa agccagtaca ccggccgccg
      481 tgcgcgtcgc gtcatggact cctcccggcc ggaacacgcc cccggcactc cggggcgact
      541 gcggcacgtc tgttccggcg ttacgagcga tcgagcgatt gttgaaacag catatacagc
      601 gtcgggtttc cgccgtcaac cactcccaac cggtccattc cgtctgacgg aacgatcagc
      661 ccgggagcca attgttagtc gcacggaggg ggggccgggt tgcgggattc cgtccccccc
      721 ggcgccgccg gtccggcgcc gggggatggg ttccctgcgg gcaggcggcg ccgggggatt
      781 ggcttcctac gggcagggcg gcgcgccggc gcggctcacg accctcttcg ggtgcccggc
      841 cggcggacag ttgccgtagg gggccggcgc cagcggcgcc ccggtgaggg tcaggcagac
      901 ctgtccttcg gccgcctgct gcgccgtcgt gggcgccgga caggttcccc cgccgggaca
      961 atcgaccacc gactgacaca gcttcccggg actgcttccg ccggcgcagg tcgagtgatg
     1021 gccgcagcct ccctgccccg cacttccggc gtccgtggag cagaaggtgg ccgacgccac
     1081 gcacgtgccg ccgggacagt tcgcgttcgt cgtgcacggg gcgccggcat tcgtccccac
     1141 ctcacaccag cccgggttgc tgcagatcgc cgggttggcg cagccgtagg cgtcgaggtt
     1201 ggcccctccg gggctgttcg ccgacgcctg gtagtagagg accgccccga ccgccggatt
     1261 cggatcctgc gcctgcgtca gcgggccgga ggtgtggttg aacccggtgg ccgccgaggt
     1321 cggagcgagg ttcgacagga aacagggacc ggagagcccg ggaagcgtcc agaaggagcc
     1381 ggagagggtc cccttcggcg caggactggc cgggacggtc cccttcacga cgtcgagccc
     1441 gtcgtcggag gcagcccaca gccgcaggac ggtgacgtcg tccgccggtg tcgcggtcac
     1501 ggcgcaggcg ccgccagcgc cgcagtcggc ggcggagttg cagggggccc cgacggtccc
     1561 ggcgctgcag gcaccgcggt agtagacgac accggcgccg gggtcgaacg acccgccgcg
     1621 ggcgccgaac accacgtccc ccccttcgcc gctccggacc ggcaggtcga tcgacgcgcc
     1681 ggcggcgctg ctgcagccgg ggtcgctggt gcagcggacg agcgccgtgt accgggtggt
     1741 ccgctccggg ctgtcgttga agaaggcgtt gccgctccac ggctgcacga ccatcccgtc
     1801 ccgcaggaac tggtactccg cgtagccgtt ggagcagccg ccggggaagc tggacccggc
     1861 cgcccccacg cgaatcggcg agcccgtggc cgggatgcgg gtgaccccgt cgatcgcgac
     1921 gcccgccgcg tcggtcacgg tcagaacgac cgccgtcccc atgtcgccga cggcggcatc
     1981 gcagggatcg cccggacagg tcccggtcct cggcgtgttg tcgcggagca gcgagacctg
     2041 gctcgtgacc gctccggtca gcacgatgtc gtcgagccac cagccgtcgt cacccgtcaa
     2101 attggcccac gtcccaccga tctcgtaata cgagccgaag tcccctccga aattccatgt
     2161 ctcggcgatc cagcggatgc ggatccgctg gccgatgtag ggatcgagat ggaatcgcgt
     2221 ctgcacccag acgccgagcc cggacggctc gacgacgcct ggaccggtgc agtcccccga
     2281 gctgatggag ctcgtcccgg tcgtagatcc gcagcgagac caggccccct gcgggtaaca
     2341 gatcgtctcg tgcacgccgc gcggattcgg cggagcggtc cccgtgtcgg tcggagtgaa
     2401 cacgcagtag taggagccga attccgaaaa ggacatcggt ttgtgttcgt agacgttctg
     2461 atacgggacc agcttgtccc agaatcccca cacgtccacg cccggatctc cttcgagatc
     2521 ggcctggatc tgcacgtcgc cgcaatcctg acaagtgtgc gccaccgtga agccggtgtt
     2581 gttgtcgatc agccgggcga tgtggaagaa cgagaagtcg agatcccccg ggcgcggctg
     2641 catcgccaga ttcaccggcg gggtcacgaa cccctgcagg gtacgaaggt gagtcgcatc
     2701 acccgccgtg gtcgagagga aatgcgcccc catgtgcaac gagttcggcg ggctcagcga
     2761 atgtgtcccg tccgtcggag tgttgtagga gcatccaccc acgcaggacc ccgattcgag
     2821 gttcggacag ttgatcgcac cgggcgggca gtgcaggtgc cagtccatcg gaaaatcggg
     2881 gtcgagggca cacaaggggg ttcccggcgc cgtgtcgaat ccaccacaag tgaccgccgc
     2941 gacccggttg agcccggcgc cggagtcgct gccgcgcagg tacgccccgg agtagcagac
     3001 cgcgcccggg ggattggccg ggcagtcggc cgccgtgatg cagttcgtga gcggcgcgtt
     3061 gctgcacgtg cctcggtaca ccccgggtgc gatcggctgg aggaaggtgt ccttgacggt
     3121 gaagttgccg tcgccgtccc gatcgaggtc gaacttctcg acgaccgtcc catcatccga
     3181 ggttcccgcg atcccgtcag gtcccagggt gaactgctgc ggaccgaagg ggctgtcgat
     3241 atcggcgagc agcgagaagg cgatcggtgc ggaggtgccg agcgcgccgg cggccgtgat
     3301 ctggagcccg aggttgatcg tcgggaacgg cgccgggggc ggcatggcct gcagggcgtt
     3361 gctggcgacg aaggtgaacc cgggctgccc gggatcgagg cttccgaggg tcaccgtcgc
     3421 attgttcggc aggctcgcga cgcggacctg ggtctcggtg atgcaggcga cgtccggatc
     3481 gctcgaggtg agcgtgaaca cggcgtcctg aagcgcaggg ccgagattcc ggatctgcag
     3541 cgacacgcgg cccgtctcgc cggtgtcggg gtaaggatcg tggtcgccat cgagatcgag
     3601 gtaggtcgcg ccgaggatcg ccacgttgac gagcggctgg gtgcggcagg cgaccgtccc
     3661 ctggaacgtc acgacgtcgt ccggcacgcc gtccccggcg tcgtcgaagg cgccggccgg
     3721 atcgcaggcg tcgccaaggc cgtcccggtc gcggtccggc tgggcaccgc cggcgacgac
     3781 cctcgggttg gccgtcatgg cgcaggaatc ggccagatcc tggaccccgt cggcgtcacc
     3841 atcgtcgtcg acggatgagc agccgccgga gctggcgagg taccactggc acacgtcgcc
     3901 gaggccgaac gaattggcgc atgccgcctg gctcgtctcc ggaaccgaac acgcggcacc
     3961 gttcgcgcgg caacggtacg ccaccgccgc gacggcgcag tcgccgtcgc aggcgttccc
     4021 gagaccgtcc tgatcgctgt ccaactgatt gttcggcccg agcagcgggc cgttcggtgc
     4081 gatgacgcag ttgtcgttcg agtcgatgac gccgtcggcg tcgcgatcga cgtcggcgta
     4141 acactgcgcg tgcgtgctgc aggcctgacc gatcttcggg gtcggtgaga cgaagttggc
     4201 ggagcagacg ccgggagccg cgcagtcggc ggcgacggtg cagacgttgc cgttgttgct
     4261 cccgccgatg cacctcaggc cgcccgcagc ccccagattg ccctgggtgc agacgttgtt
     4321 gaagcaggac cggatgcagt tctcgtcggt gacacaagcg tcgccgatgg catcgcccgg
     4381 ccgcgtgcag aggccggaca cgcagtcggc ggcgccggcg gacgttccaa aaatgacgcc
     4441 cgacgtcttc agcgtgtcac acaggtcgcc gcggccgtct gcgtcgatgt cggactggtt
     4501 cgggttgcgg acgtcaggac aggtgtcggc accgttcggc aggccgtcgc cgtcgatgtc
     4561 gtcgaactcg caggcgttgc ccacgccgtc ggcgttggtg tcggcctgcg gggtgttcgc
     4621 gacgaccggg cagttgtcgc aaaggtcgcc cagcccgtcc gcatcagcgt cttgctgaag
     4681 cgggtcgtag acaccgggac agtgatcggc ggcgtccggg atcccgtcgc cgtcgatgtt
     4741 gtcgaaggcg ctctctcccg cctggccgtc gcggtcaccg tcgcacagcg gatcgaagta
     4801 ctcgaccacg aagccactgt ccgacccggc cgacacgtgc acgcgcgtgg cgtccgcggg
     4861 gagcgtcgtg aacgagacgc gcccgcggaa gagtcccggc tgccccggca cggccggaag
     4921 aacgaaatgc ttcgccgcgg gtgtcaggat gagcggaccg ccactggtgc cgtcgctgtc
     4981 ggtcgccacc gccacctcga ccgatgcgag cgcggccgtg caggtgccgc cggagccgca
     5041 ctgcgcgttg gtcgtgcagg cgccggccag cggcaccgtg ttgccggcgc cgatggtgcg
     5101 gcacttcgcg tcgcgcaccg tgatctcgac cgtctcctcg cactcgtaga ggctcgtccg
     5161 gtccaccgtg accgtggcgc actgcccgcc ggccggctgg ccgatgccat tgaagcgggc
     5221 acaggccggc tgggcgccga gagccgcctc gtcctgcggg tggtactcct tccactcgaa
     5281 gaccgcgtcg tcgatcgact tgccgtagtc ggtcgtcccg gtcgtccctt ccatcgccca
     5341 gaagccgatg ccgatctgcc agcggttgcc ggccttgccg gggaggaaga agaagaagtt
     5401 ttcgtgaggt ccatcatcga tcaggctggc gaaaccgccc tcgtagccga tcagggtggc
     5461 gtcccagttc cggaccggac cgttgaccgt gttgaccgcc gcgaagcacg cgccgccgcc
     5521 ggtgatgcac gggtcgccgg cgttccccgg gtcgcaggtg gcgtcgttct gtgggccacc
     5581 gtcgcagacg ccgatgaccg gtgcgcccgg aaccggatag ggcaggaagt ccgggatggc
     5641 cgtcgggatc ggcgtggagc tgtcaggatt ggtgttctgg gtcgcgccgc tgaagccggt
     5701 ctcgtcgccg tccaggttca ccgaggcgtt cgggttggtg aacggcccga aggtgcgctg
     5761 gaatggaatg gtcgtcgtcg ggtcgatccc gtcgccgggg aagtactgcc cggcgtcgcg
     5821 gaacatcccg tacggccagc cgccggtccg ccgggtgtag tactggtcca tctgctgggc
     5881 cagcaggctg ttggcgttgt cgctgtcgat gtcgttgtcg atgttgacac cgccgccggc
     5941 gtagccgtcg ttgatctgga tgttctggtt gtacgcgaag cgctggaact ccacggtgta
     6001 cgagaagccg cgcgcgtcgt tgttctggtt caccttggcg acgatcggcg actccaggac
     6061 gtccatcagg agctcgacct tcgccggggt gttcggatcg tgcggctgcg tatggttgtc
     6121 gcagggcgtg gccaccccgg acggggtggt gggatcgccg tcacccgtgt gccagatccc
     6181 gaacttgttc gctccgccac tggtctggaa gccgcacagc ccggaggtct tgtactccca
     6241 cagggggttg acgttgacgc cggccgcggt gctgcccggg tgacggaacg ccgcccagcc
     6301 gccgctgttg ttgtcgaagt tccacggcac gcggtggttc gggtcggcgg gattgttgga
     6361 gttcagaatc ccgcggtcga gcacgggatt cccgtcgggg tccacgttgg ggacgacgtt
     6421 gcgctcggtc ccgctgaagg tcccgctgag atcgaagtcc tcccctatct cgttggtgaa
     6481 gtggcccaac ggggcgcccg tgccgctgaa caggctcgcg aacgtcacgt cctcgcgcgc
     6541 cacgatgaac ccgaggagcg ggtcggtcgt gtcgggcgga tcgaccacga agttgcggtt
     6601 gatgtcgcgc acctggcggc cgccgttcgg gaagtcggtc gagtagaaga aggtctcgcg
     6661 gtccgagttg agagcgtggg tgaacgagta ggactgtctc gccagccgcg ttccgcggct
     6721 ggaggagtcg aggtcgaggc gcatggtcac gacgcggttg gcgaccgaca gcgcgttcac
     6781 cacggtgggg tccacgtaca cctggaagaa gacggcgttg acgccctgcg ccggcaggat
     6841 ccccaggtga cgcggcgaat cgaggacccg caccgccgca gcaccgggac cggatggcgt
     6901 cagcgtggcg acggcgtcgg cgtagccggc gtcgcgcgag cggttcctga gggccacgcc
     6961 atagctcacg acctccccgg cgtcgaggaa ggcgtcctgg tcgcagccgc cctggatcga
     7021 gacctgctct ccgaccgcgt tcccacctgc cgtcaggaac ggggtgaaga cgagatcggg
     7081 agaacagcgg acggaggctt ccgcctcgac cgccggctgg ccgggaggcg cgtaacgggc
     7141 ccgcaccctc tggcccgtgt cgccttcgag gatgccgttg ccgctcagcg ctggtccggc
     7201 gagccgcacc gggatcggcg ccgatgcggt cttccccgga ccgagcagcg tgaacgacag
     7261 gccggtctcg gtgtccagca ccgttccgtc ggcggcgacg accgtgaagg tcgtcgccga
     7321 gctcgacaag gccgcggagg ccgccggcgt cgtatcgaga atcgtcgcga cggcgtcctt
     7381 gccgcagtcg tacgcgatcc ggtcgaaatc cacccggctc tgcgggaacg tctgcgcccc
     7441 cagcgccggc gccgcctcgg cgaggaacac cgggccggag accaccagcg cgtacggctg
     7501 tccgagctga tcgagcaggc cgttggcgtt gtcgtcctcg cccgcgtcca gccggccgtt
     7561 gttgttgtcg tcctcgttct ggtcgtcgtc ctcggcgacg gtcggggcgg tgatggtgat
     7621 cgaccccgga atcgcgccgc caaggccgcg cttcacggtg acgcgccaca gcccggtata
     7681 gaggaccgac tcgttgaaca cgaagtcgat gttcgtgtcg gcgctcaggt gcaccgcctc
     7741 ggtgttgttc cgcttctcgt gcttctccac cggggtggtg gaggtgcggc ccagggacca
     7801 ctggtcgaag ctcgcgttgt tgcggccgcc gtcgtagcgg ttcca
//
LOCUS       DASRIQ010000565         4513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_755472, whole genome shotgun sequence.
ACCESSION   DASRIQ010000565 DASRIQ010000000
VERSION     DASRIQ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755472"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1167)
                     /locus_tag="VFD06_14000"
     CDS             complement(40..1167)
                     /locus_tag="VFD06_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN04692.1"
                     /translation="MDHVRPSGTKPPLVTLDGVTRRYDRGVKALDGVSLEIRAGEWLA
                     IMGPSGSGKTTLLNILGGLDRPDEGRVVVDGADIVRLPRTALTRYRRETVGLVFQAHH
                     LIPYLTAVENVMLAQYLHSLADEKEAAAALEEVGLGARLRHLPSQLSGGEQQRVCIAR
                     ALVNRPRLVLADEPTGALDAENERIVLEIFDRLRARGQTIVMVTHDLVVGRRADRQIQ
                     LEHGRVAGEFLTPSQDEEAIDEVLEFLWLRQEGDPLAHQTCAIGASLATPLVLDRMRD
                     RGILDPAATPRFTAEGHDRAERLIRRQRLAETLFTQTFQMHEAEVEAEACYFEHILSP
                     AVTDSICSFLGHPPACPHGKPIPRGACCAAADLIGVDRRSS"
     gene            complement(1173..2330)
                     /locus_tag="VFD06_14005"
     CDS             complement(1173..2330)
                     /locus_tag="VFD06_14005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN04693.1"
                     /translation="MSARLFGRSLGRRGVTFFIALVAITGGSGVAATMLTLKADLRAK
                     MTREIRRYGENLALAPAEDRAGATLDEAAVRRAIGDEGAPVLLARGEIGRRDGAAAGA
                     AVVGVDFAAHQRLHPSWRIDGHWPAPDASPAPGAAPQPNASPVPALLGASLAKRLGLA
                     PGDDAALSLESGGGPIRLGVAGVARTGESEEDQVFVPLALLQSRTGLPGRISLAALFV
                     PGGAGEIEAAARRLEREVPTARATPLRPIAAAQGAILDRLDRMTFLMSLIILALAGLC
                     LGTTLFTLVVEREAEIGLMRSLGAGDGDVARIVLGEVTLLGLAGGTIGLLLGAVAARL
                     VGHRLFGAPIDPRLSVVPMVLGIALVVSWIAVLVPLRRALLIRPAAALRAE"
     gene            complement(2376..3698)
                     /locus_tag="VFD06_14010"
     CDS             complement(2376..3698)
                     /locus_tag="VFD06_14010"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04694.1"
                     /translation="MFLRVLGDAFARQKRRKAIVFVAVALGTAAASAPADIALDVGDR
                     MSRELKAFGANLVVVPRGASQPVRVGGTDFTALSVPAWLRADDVLRVKDNFWKNNILA
                     FAPLLDAPADVTAAGGGEARRAILRGAWFDRPFRLDGEKDAAGPAATSGLRRLQPFWE
                     VEGRWPDETGTGNEALVGTRLAASLGLRPGSLLEARVHGGGTAAPLLVVGILTTGGDE
                     EDMVLLPLETVQRAAGLEGRLSRVLVSALTTPESAVYERKGLDPRSLSPAEFESWSCT
                     PFVSSIAFEIEESWPGAEARVVRRVADAEGAVLGRASGLMTLVAVMGLFGAALTVASA
                     LTAGVLERRVEIGLLKSLGARNSMVVALFLSEAAILGIAGGLAGSAAGAWLARVVSAS
                     IFGTPVAIRPASVPLAVACALAITLLGALLPVRRIAGFRPAEVLREAV"
     gene            complement(3709..>4513)
                     /locus_tag="VFD06_14015"
     CDS             complement(3709..>4513)
                     /locus_tag="VFD06_14015"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HZN04695.1"
                     /translation="RFFKVTEIVLIVLAAQLFIGGLHELGEAGILPVGRDEMRIIGPI
                     VKSDALVFAALLALPLFAIALPGRRDRERAAEAARLEGPERRLALASLGRETRWRRIL
                     SVAGVVVIAALTVSHAFSRLPAALDPPRMLAIDAAGSDGGTIRLPTTGLDDGRLHRFG
                     VAIDGTVVRFIVMKADGRLVATFDACIVCGAKGYIEKQGRLVCLACAADINTATLGTG
                     GGCNPIPLRYAESDGALVIAASDLRAEAHVFRQDQDGDGTPDAPKRAGP"
BASE COUNT          644 a         1662 c         1683 g          524 t
ORIGIN      
        1 cttcgatcac ggaagggccg gtcgaccgag tcgtcgggtt cacgagctgc ggcgatcgac
       61 tccgatgagg tccgcggcgg cgcagcaggc gccgcgcggg atcggcttgc cgtgcgggca
      121 ggcgggagga tggccgagga aggagcagat cgagtcggtg acggcggggg agaggatgtg
      181 ctcgaaatag caggcctccg cctccacctc cgcctcgtgc atctggaagg tctgcgtgaa
      241 cagggtttcc gcgagccgtt ggcggcggat gaggcgctcc gcccggtcgt gcccctcggc
      301 cgtgaagcgg ggagtcgccg cggggtcgag gatgccgcgg tcgcgcatcc ggtcgaggac
      361 gagcggtgtc gccagcgagg cgccgatcgc gcaggtctgg tgcgccagtg gatccccctc
      421 ctgccgcagc cagaggaact ccagcacctc gtcgatcgcc tcctcgtcct gcgacggcgt
      481 caggaactct cccgccaccc gcccgtgctc gagctggatc tgccggtcgg cgcgccggcc
      541 gacgacgagg tcgtgcgtca ccatcacgat cgtctggccg cgcgcccgga gccggtcgaa
      601 gatctccagg acgatccgct cgttctcggc gtcgagcgcg ccggtcggct cgtcggccag
      661 caccagccgc gggcggttca cgagcgcgcg cgcgatgcag acccgctgct gctctccgcc
      721 cgagagctgc gacggcaggt ggcgcagccg ggcgcccagc ccgacttcct cgagcgccgc
      781 ggccgcctcc ttctcgtccg ccaggctgtg gaggtactgc gccagcatga cgttctcgac
      841 ggcggtcaga taggggatga ggtggtgcgc ctggaagacc aggccgacgg tctcgcggcg
      901 gtagcgggtg agcgccgtgc gcggcaggcg gacgatgtcg gcgccgtcca cgacgacgcg
      961 cccctcgtcc ggccggtcga gcccgccgag gatgttgagc agggtcgtct tccccgatcc
     1021 ggaggggccc atgatcgcca gccattcgcc ggcgcggatc tccaggctga cgccgtcgag
     1081 cgccttcacc ccccggtcgt agcgtcgcgt gacgccgtcg agcgtgacca gcggcggctt
     1141 cgttcccgag ggacgcacgt gatccatgcc gctcactccg cccgcagggc cgcggccggc
     1201 cggatgagga gcgcccggcg cagcggcacc aggaccgcga tccaggacac gacgagcgcg
     1261 ataccgagca ccatcgggac gaccgacagg cgcgggtcga tcggcgcgcc gaacagacgg
     1321 tggccgacga gacgcgccgc cacggcgccc agaaggaggc cgatggtccc ccccgcgagg
     1381 ccgagcagcg tgacctcgcc gaggacgatc cgggcgacgt cgccgtcccc cgccccgagc
     1441 gagcgcatca gcccgatctc cgcctcccgt tcgacgacga gggtgaagag ggtcgtgccg
     1501 aggcagagcc cggcgagcgc caggatgatc aggctcatca gaaaggtcat ccggtcgagg
     1561 cgatcgagga tggccccctg cgccgcggcg atcgggcgga gcggggtggc ccgcgccgtc
     1621 gggacctccc gctcgagacg gcgcgcggcc gcttcgatct ctcccgcccc cccagggacg
     1681 aacaacgccg cgagcgagat gcggccgggg agaccggtgc gtgactggag gagcgccagg
     1741 ggcacgaaaa cctgatcctc ctccgactct ccggtccgcg ccacccccgc gacgcccagg
     1801 cggatcggcc cgcctcccga ctccagactc agcgccgcgt cgtcgcccgg cgcgagcccc
     1861 aggcgcttcg cgagcgacgc gccgaggagc gctggcaccg gggacgcgtt cggctggggc
     1921 gccgcgccgg gagcggggga ggcatccggg gccggccagt gcccgtcgat ccgccaggag
     1981 gggtggagcc tctggtgcgc ggcgaagtcc acgccgacga cggcggcgcc ggcggctgcc
     2041 ccgtcgcggc ggccgatctc cccgcgcgcc agcaggaccg gcgcgccttc gtcgccgatg
     2101 gcgcggcgaa ccgccgcctc gtcgagcgtc gcgccggcgc ggtcctccgc gggcgcgagc
     2161 gcgagattct cgccgtagcg gcggatctcg cgggtcatct tggctcgcag atcggccttg
     2221 agcgtcagca tcgtggccgc gacgccggag cctccggtga tggcgacgag cgcgatgaaa
     2281 aacgtcacgc cgcgccggcc gagggagcgg ccgaagagtc gcgccgacat cctccaccag
     2341 ccgcgccgcc cgctcaacgg cttgacccct gcccgctaga cggcttcacg cagcacctcc
     2401 gccgggcgga acccggcgat gcgccgcacc gggaggagcg ccccgagcag cgtgatggcg
     2461 agcgcgcagg cgaccgccag cggcaccgag gccggccgga tcgcgaccgg cgtgccgaag
     2521 atcgaggcgg agacgacgcg ggccagccag gcccccgcgg ccgagccggc gagcccgccg
     2581 gcgattccga ggatcgccgc ctccgacagg aagagggcga cgaccatcga gttgcgggcg
     2641 ccgagcgact tcaggagacc gatctcgacc cggcgctcga gcacgccggc ggtcagggcg
     2701 ctcgcgacgg tgagggcggc gccgaacagc cccatcaccg cgacgagcgt catgagcccc
     2761 gaggcgcggc cgagcaccgc cccttcggcg tcggccaccc gccgcacgac gcgcgcctcg
     2821 gcgccgggcc aggactcctc gatctcgaag gcgatcgacg agacgaaggg ggtgcaggac
     2881 caggactcga actccgccgg actcagcgag cgcgggtcca gtcccttgcg ctcgtacacc
     2941 gccgattcgg gggtggtgag ggcgctcacg aggacgcgcg acaggcgccc ctcgagcccg
     3001 gccgcgcgct gcaccgtctc gagcgggagg aggaccatgt cctcttcgtc gccgccggtg
     3061 gtcaggatcc cgacgaccaa aagcggcgcg gcggtcccgc cgccgtgcac acgcgcctcg
     3121 agaagcgacc cgggccgcag gccgagcgag gcggcgagcc gcgtgccgac cagcgcctcg
     3181 ttccccgtac cggtctcgtc gggccagcgc ccctcaacct cccagaacgg ctgcaggcgg
     3241 cgcaggccgc tggtcgcggc cgggcccgcc gcgtccttct ccccgtcgag gcggaacggc
     3301 cggtcgaacc aggcgccacg caggatcgcc cggcgcgcct cccctccgcc cgcggcggtg
     3361 acatccgccg gtgcatcgag gagcggcgcg aacgcgagga tgttgttctt ccagaagttg
     3421 tccttcacgc gcagcacgtc gtcggcgcgg agccaggcgg ggacgctcag ggcggtgaag
     3481 tcggttcccc cgacgcgcac cggctgggag gcgccgcgcg gcacgacgac gagattggcg
     3541 ccgaacgcct tcagctcgcg gctcatgcga tcgccgacgt cgagggcgat gtcggccggc
     3601 gcggaagcgg ccgcggtccc gagcgccacc gccacgaaga cgatcgcctt gcgccgcttc
     3661 tgccgggcga aggcgtcgcc gaggacgcgc aggaacatgg acgactcctc agggccccgc
     3721 gcgcttcggc gcgtcgggtg tcccgtcccc gtcctggtcc tggcggaaga cgtgcgcctc
     3781 ggcgcgcagg tcggacgccg cgatcaccag cgcgccgtcg ctctccgcgt agcgcagcgg
     3841 gatcggattg cagccgccgc cggtcccgag ggtcgcggtg ttgatgtcgg cggcgcaggc
     3901 gaggcagacg aggcgcccct gcttctcgat gtaccccttc gcgccgcaga cgatgcaggc
     3961 gtcgaacgtg gcgaccaggc gcccgtcggc cttcatgacg atgaagcgga ccaccgtccc
     4021 gtcgatcgcg acgccgaagc ggtgcaggcg cccgtcgtcg agtcccgtcg tgggcaggcg
     4081 gatcgttccg ccgtcggagc cggccgcgtc gatcgccagc atgcggggcg ggtcgagggc
     4141 ggccggcagg cgcgagaagg cgtgcgacac ggtcagcgcc gcgatgacga cgacgccggc
     4201 gacggacagg atgcggcgcc agcgggtctc ccgcccgagc gaggcgagcg ccagccggcg
     4261 ctccggccct tcgaggcggg ccgcctccgc ggcgcgctcc cgatcgcgcc ggccggggag
     4321 ggcgatggcg aacagcggca gcgcgagcag ggcggcgaac accagcgcgt cgctcttgac
     4381 gatcggcccg atgatgcgca tctcgtcgcg tccgaccggc aggatccccg cctcccccag
     4441 ctcgtgcagg ccgccgatga acagctgtgc cgccagcacg atcaggacga tctcggtgac
     4501 cttgaagaag cgg
//
LOCUS       DASRIQ010000566         8004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2299185, whole genome shotgun sequence.
ACCESSION   DASRIQ010000566 DASRIQ010000000
VERSION     DASRIQ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2299185"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VFD06_14020"
     CDS             <1..1309
                     /locus_tag="VFD06_14020"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZN04696.1"
                     /translation="EIYRDIVAELAPETPRNRLMIASGMRSWLRVPVRLSGGEVRGGI
                     AFAHREPARYTPADAEVALRVADRIALALAHHRLSEEARTAAEARERAERLEATVETL
                     TRALEAQGRGRVVGVSDSWKEILTQVSRVASSGTTVLITGESGTGKEVVAHLLHEGSP
                     RARKPFVAINCAALPDQLLESELFGHERGAFTGATAAKVGRVEQAAGGTLFLDEIAEM
                     TPLVQAKLLRVLQEREFQRLGGTRAMKADVRVIAATNRDLQAAMERGEFREDLFYRLA
                     VFEIAIPPLRARREDILPLAESIVEEFGRTMGRPAAGISRDAREWILAHSWPGNVREL
                     RNAIERAILLCDGGLITREHLPAVVGRPAAARAAAARQGRAPALRDATGALPPGGVDL
                     EAMEREFVEKALKQVRGNKTKAAQLLGLTRAQLYSRLEKYGLS"
     gene            complement(1343..2089)
                     /locus_tag="VFD06_14025"
     CDS             complement(1343..2089)
                     /locus_tag="VFD06_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZN04697.1"
                     /translation="MSEARITAVLVEDEPEARTNLRAWLQDVDWIAVAGEAADGKSAV
                     ALIDGVRPDLVFLDVQLPEMTGLRVLESVRHAPEVVFTTAYDQYALAAFEIGALDYLV
                     KPFGRERFMKALSRVKQRLGGAGAPSAPERARAAFAPGFLTRLFARRGERIVPILARD
                     IRRIAAQGDYAEVHTSTGTFLLHVSLKELAARLDPERFVQVHRSHIVNLDAIDHLKPY
                     DERRLVLVLKEGGEIVASRAASERLRHGVR"
     gene            complement(2086..3333)
                     /locus_tag="VFD06_14030"
     CDS             complement(2086..3333)
                     /locus_tag="VFD06_14030"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN04698.1"
                     /translation="MSHLLCWHPTGVRTRPCRGIGGRPDRPGEGPVGTTDGADSRGLR
                     GHNAAVPNGDDAIRGPMRPAGRASGRGAAVAWGTAVLAYFSITLAAILMTSSLTLWVG
                     VRSALLAVLPDCVLAPLALRHTRRAPWGRVPAWRFALHHLVGGALFVLGSTVLYLGEF
                     FVERRVFNGAWPALPPLAGFVYKGFGSILVYATVCAIGSAVEQARRAARAEVLSAEAR
                     LAALRARLNPHFILNLLHTLMGLVGRDPASAQAALEQLGDVLRYALRVQSQGLDEVRL
                     REEWDFVERYLALERLRLGDRLRIRLAVEDGLLDAVVPVFSLQPLVENAVRHAIAPRA
                     TGGSVEVTAARAGSAVVLMVEDDGAPGSAGAGARADGAGIGLRLIRDRLSALYGDAAR
                     LETGRSSLGGFRAAVTVPLGEPL"
     gene            3332..4558
                     /locus_tag="VFD06_14035"
     CDS             3332..4558
                     /locus_tag="VFD06_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04699.1"
                     /translation="MRLSTHSALVWFLVLLVLIGIAAAVPGGARAKSPSIDGVSIDDF
                     EDRNLVGSSGLAWVPLGDDLFGGPTRMRLEPLRGGASGSKGAMRVTATIGDGPGSVAG
                     AWISIVPGGRTADLTGIDGVRLSVRGSGEVLVGVRRGGPMGGVNFMTTVTAGPDWRTV
                     LVPFDRLKPQGQGLEATGWDPRDGRWLGVTSVPGAAGEFRVDVDDVAWYANDRKVPSP
                     AWDAKDTPTFRALEADDPAPLAALPWRELARDPEGDGKPGLPDARALFVAPDPRRPLT
                     WLRVDLQGPIPPSWMGVNLVLDTDGDPADGTPWWGNDKSFRFDRLVTAWVFSVDRHFE
                     GSVGIATAEQAAAGLVTNDEEVHLALDRSARRVYLGVPSATLAAGTRTVAAVGSAFIF
                     SDDLPASGGVEVGPIR"
     gene            4744..4998
                     /locus_tag="VFD06_14040"
     CDS             4744..4998
                     /locus_tag="VFD06_14040"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HZN04700.1"
                     /translation="MSQRNVERVIGRLVTDEKFRRRFESDPVKTLEEMAATGAELTDV
                     EKRALAGLDARLVARFADAIDPRLQKICLTEDRADGPLPH"
     gene            5038..6162
                     /locus_tag="VFD06_14045"
     CDS             5038..6162
                     /locus_tag="VFD06_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN04701.1"
                     /translation="MLKRMIIMLAVVVGLLSIIGFVKYRQISAAIAGGASYQPPPEAV
                     TTVVAGSIEWDQTISAIGSVTPVQGVTVSADLPGIVQSIAFESGRSVKAGQVLVHLDT
                     SQERAQLASAEAKYRLTGLELERARGLRDEKVVSQADLDAAEAANVQAEAAIAEIKAT
                     IGRKTIRAPFSGILGIRQVNLGQYLNGGDPVVSLQSLDPIYVNFSVPQGDLPRVRLGG
                     DVRVTSEGLDEAVTGKVTAIDSVVDAATRNAQVQALVHNPGHKLKAGMYARAEVTLPD
                     RTTLVPLPASSILYAPYGDSVFIVEEIQGKDGKRYKGVRQQVVKLGGSRGDQVAVLSG
                     VKPGEEVVSSGVFKLRNGAAVEVNNEVQPSNNTNPKPEES"
     gene            6162..>8004
                     /locus_tag="VFD06_14050"
     CDS             6162..>8004
                     /locus_tag="VFD06_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN04702.1"
                     /translation="MKFTDLFIRRPVLAIVVNLVILIAGLQSIRSLTVRQYPRSDIAT
                     ISVATVYVGANADLVRGFITTPLERVIASADGIDYLESSSAQGISTITVHLKLNYDTN
                     AALTQIQAKVAQVRNQLPPEAEAPVIEVQTADTQFAAMYIGFSSESLEANQITDYLVR
                     AVQPKISAISGVQRADILGNRNFAMRIWLKPDRMASYGLSASDVREALANNNYLSALG
                     RTKGSMVSVNLVANTDLRTAEDFRQMVVKEKEGSVVRLGEIADVQLGAENYDDQVRFD
                     GQTATFMGIWVLPTANSLDVVTKVRAALPDIEAQLPAGMKVAVPYDSTLYIRNAIDEV
                     LKTLSETLLIVIIVIFLFLGSFRAVLIPVIAIPISLIGAVFLMLAFGSTINLLTLLAI
                     VLSVGLVVDDAIVMVENVERHLALGKPPVQAALVAARELVGPIIAMTVTLVAVYAPIG
                     IQGGLTGALFREFAFTLAGAVLISGVVALTLSPMMASRLLKPGATEHGLPGRINRGFE
                     RLQRFYTSWLSGTLAYRAVVLTLWVIVVALIAPFYMFSQKELAPTEDQGVVFGIVQAS
                     ANSTLDQTKLFADQAYQVYKSFPETKNTFEITNANGGFGGMVVKPWSE"
BASE COUNT         1224 a         2834 c         2796 g         1150 t
ORIGIN      
        1 cgagatctac cgggacatcg tcgccgagct ggcccccgag acgccgcgta atcgcctgat
       61 gattgccagc gggatgcgct cgtggctgcg ggtgccggtc cgcctctccg gaggagaggt
      121 gcggggcggg atcgccttcg cccaccgcga gccggcccgc tacacgcccg ccgacgccga
      181 ggtggcgctc cgggtcgccg atcggatcgc cctggcgctg gcgcaccacc gtctttcgga
      241 ggaggcgcgc accgccgctg aggcgcgcga gcgggcggag cggcttgagg cgaccgtcga
      301 gaccctcacg cgcgccctcg aggcgcaggg gcgcggccgc gtcgtcggcg tctccgactc
      361 gtggaaggag atcttgacgc aggtgtcgcg cgtcgcctcc tcgggcacga cggtcctgat
      421 caccggcgag tcggggaccg gcaaggaggt cgtggcccac ctgctgcacg agggatcgcc
      481 gcgcgcgcgc aagccgttcg tggcgatcaa ttgcgccgcc cttcccgacc agctcctcga
      541 atccgagctg ttcggccacg agcggggggc gttcaccggg gcgacggcgg cgaaggtcgg
      601 ccgggtggag caggcggcgg gggggacgct gttcctcgac gagatcgccg agatgacccc
      661 gctcgtgcag gcgaagcttc tgcgcgtgct ccaggaaagg gagttccagc gcctcggcgg
      721 gaccagggcg atgaaggcgg acgtgcgggt catcgccgcg accaaccgcg acctgcaggc
      781 ggcgatggag cggggggagt tccgcgagga tctcttctac cggctcgccg tcttcgagat
      841 cgccattccg cccctccgcg cgcgccgcga ggacatcctg ccgctggcgg agtcgatcgt
      901 cgaggagttc ggccgcacga tggggcgccc ggcggccggc atctcgcgcg acgcgcgcga
      961 atggatcctc gcgcattcct ggcccgggaa cgtgcgcgag ctccggaacg ccatcgagcg
     1021 agcgatcctt ctgtgcgatg gaggcctcat cacccgcgag cacctgccgg cggtcgtcgg
     1081 ccggcccgcg gcggcccggg ccgcggccgc gcgccagggc cgcgcaccgg cgctgcggga
     1141 cgcgaccggg gcgcttccgc cggggggcgt cgatctcgaa gccatggagc gggagttcgt
     1201 cgagaaggcc ctgaagcagg tgcgcggcaa caagaccaag gcggcgcagc ttctcggact
     1261 gacacgcgcc cagctctact cccgcctgga gaagtacggc ctcagctgac gctcgcgcgg
     1321 accgacggcg cgcttcctgc gctcagcgta ctccgtgccg cagccgctcc gacgccgccc
     1381 gactcgcgac gatctcccca ccctccttga gcacgaggac gagccggcgc tcgtcgtacg
     1441 gcttgaggtg gtcgatggcg tcgagattga cgatgtgcga gcggtgcacc tgcacgaagc
     1501 gctccgggtc gagccgcgcc gccagctctt tcaggctcac gtgcagcagg aacgttccag
     1561 tggacgtgtg cacctcggcg tagtccccct gcgccgcgat ccggcggatg tcccgcgcca
     1621 ggatcggcac gatccgctcg ccgcgccggg cgaacaggcg cgtcaggaac cccggcgcga
     1681 aggcggcgcg cgcccgctcc ggggcggagg gagcgccggc cccgccgagg cgctgcttca
     1741 cccgcgacag cgccttcatg aagcgctcgc ggccgaacgg cttgacgagg tagtcgagcg
     1801 ctccgatctc gaacgcggcg agagcgtact gatcgtaggc ggtcgtgaag acgacttccg
     1861 gggcatgccg caccgactcg agcacccgca gcccggtcat ctccggcagc tgcacgtcga
     1921 ggaagacgag gtcggggcgc acgccgtcga tgagggcgac ggcgcttttc ccgtccgccg
     1981 cctcgccggc gaccgcgatc caatcgacat cctggagcca ggcgcggagg ttggtgcgcg
     2041 cctccggctc gtcctcgacc agcacggcgg tgatccgcgc ctcgctcaca gcggctctcc
     2101 gagcggcacc gtgaccgcgg cgcggaaccc cccgagcgac gaacgtcctg tctcgagccg
     2161 cgcggcgtcg ccgtagaggg ccgacagccg gtcgcggatc aggcgcaggc cgatgccggc
     2221 accgtcggcg cgcgcgccag cgccggccga tcccggcgcg ccgtcgtcct cgaccatcag
     2281 gacgaccgcg gagccggcgc gcgccgccgt cacctccacg ctgccgcctg tggcccgcgg
     2341 cgcgatggcg tggcgcacgg cgttctcgac aagcggctgc agcgagaaga ccggcacgac
     2401 cgcgtccagc agcccgtcct cgaccgcgag ccggatcctc agccgatcgc cgagccgcag
     2461 gcgctcgagc gccaggtacc gctcgacgaa gtcccattct tcgcgaagcc gcacctcgtc
     2521 gagcccctgc gactggaccc gcagcgcgta gcgcaggacg tcgccgagct gctcgagggc
     2581 cgcctgcgcc gaggcggggt cgcgccccac gagccccatc agcgtgtgca ggagattcag
     2641 gatgaagtgc gggttcaggc gggcccggag cgccgcgagc cgcgcctcgg ccgacagcac
     2701 ctccgcccgc gctgcccgcc gcgcctgctc gacggcgctg ccgatggcgc agacggtcgc
     2761 gtagacgagg atcgatccga accccttgta gacgaagccg gcgagcggcg gcagggccgg
     2821 ccaggcgccg ttgaagaccc gccgctcgac gaagaactcc ccgaggtaga ggacggtcga
     2881 tccgaggacg aacagcgcgc cgccgacgag atgatgcagc gcgaagcgcc atgcggggac
     2941 gcgtccccac ggggcgcggc gcgtgtgacg gagcgccagc ggcgcgagca cgcaatccgg
     3001 caggacggcg aggagcgccg accgcacccc cacccagagc gtcaggctcg aggtcatgag
     3061 gatggcggcg agcgtgatcg agaaataggc gaggaccgcg gtcccccacg cgaccgccgc
     3121 gccgcgtccg gacgcgcggc cggcggggcg catcggaccg cggatcgcgt cgtctccgtt
     3181 cggcacggcg gcattatggc ctcgaagccc ccgcgagtcg gcgccgtccg tcgtcccgac
     3241 cggcccttcg cccggccgat ccggccgccc gccgatgccc cggcacggcc gcgtgcgcac
     3301 cccggtaggg tgccagcaca ggaggtgcga catgcgactc tcgacccact ctgcgctcgt
     3361 ctggttcctg gtgctgctgg tgctcatcgg catcgccgcc gccgtgccgg gcggggcccg
     3421 cgccaaatca ccttcgatcg atggggtttc gatcgacgat ttcgaggatc ggaacctcgt
     3481 cggatcctcc ggcctcgcct gggtgccgct gggcgacgac ctgttcggcg gccccacccg
     3541 catgcggctc gagccgctgc gcggcggcgc ctccggatcg aaaggggcga tgcgcgtgac
     3601 cgcgacgatc ggcgacgggc ccggctcggt cgccggcgcc tggatctcca tcgtccccgg
     3661 cggccgaacc gccgacctca ccgggatcga cggcgtcagg ctgagtgtgc gcgggagcgg
     3721 cgaggtcctc gtcggcgtcc ggcgcggcgg ccccatggga ggggtgaact tcatgacgac
     3781 cgtcaccgcc ggaccggact ggcggacggt cctcgtgcca ttcgaccggc tgaagccgca
     3841 agggcagggt ctcgaagcga ccgggtggga tccgcgcgac gggcgctggc tcggagtcac
     3901 ctcggtccct ggtgcggcgg gcgagttccg ggtggatgtc gatgacgtcg cctggtacgc
     3961 gaacgaccgc aaggtcccgt cccccgcctg ggacgcgaag gacacgccca cgttccgcgc
     4021 gctcgaggcg gacgacccgg ctcccctcgc cgctctcccg tggcgcgagc tggcgcgcga
     4081 tccggagggg gacgggaagc ccggcctgcc ggacgcgcgg gcgctgttcg tcgccccgga
     4141 cccgcgccgt ccactcacct ggttgagggt cgacctccag gggcccatac ccccgtcgtg
     4201 gatgggggtc aacctggtcc tcgacaccga cggcgatccg gccgacggga caccctggtg
     4261 gggaaacgac aagtcgttcc gcttcgatcg gctggtgacc gcctgggtct tctcggtgga
     4321 tcggcacttc gaaggctcgg tcggcatcgc gacggccgag caggccgccg cgggcctggt
     4381 caccaacgac gaggaggtac atctcgccct cgatcgatcg gcccgacggg tctacctcgg
     4441 cgtcccctcg gccaccctgg cggccggaac ccgcaccgtc gccgcggtcg gctcggcgtt
     4501 catcttctcc gacgacctgc cggcctccgg gggggtggag gtcgggccga tccgctagcc
     4561 caggtgctgc cggatcggca aactgtcgat ttctctacga tcgcaggaaa tcggggatcc
     4621 ttcatggcgg gggggcgcgc gcgcctcaac tcctgcacca tgaagcgctt gggccgagtc
     4681 tccagacccg tcacaaaggt ggcacagcct ttggttaagg gaatcaacgg gaggagactt
     4741 acgatgagcc agagaaacgt ggagcgggtg atcgggcggc ttgtgaccga cgagaaattc
     4801 cgtcgccggt tcgagtccga cccggtgaag accctggagg agatggcggc caccggggcc
     4861 gagctgaccg atgtggagaa acgggcgctc gccgggctcg acgcgcgcct ggtggcgcgc
     4921 ttcgccgacg ccatcgatcc gcgtctccag aagatctgcc tcaccgagga tcgggccgat
     4981 ggtccgctgc ctcactgagt cgtcaggcgc cgccccgtcg gccggaaaga gagtcccatg
     5041 ctgaagcgaa tgatcatcat gctcgccgtc gtggtggggc tcctctccat catcggcttc
     5101 gtgaagtacc gacagatcag cgccgcgatc gccggcggcg cctcgtacca gcctcctccc
     5161 gaagcggtca ccaccgtcgt cgcgggctcg atcgagtggg accagacgat ctccgccatc
     5221 ggatcggtga cgccggtgca gggagtcacg gtgagcgccg acctgccggg gatcgtgcag
     5281 tcgatcgcgt tcgagtccgg gcggtcggtc aaggccgggc aggtgctcgt gcacctcgac
     5341 accagccagg agcgggccca actcgcctcc gccgaggcca agtaccgcct caccgggctt
     5401 gaactcgagc gcgcccgcgg cctgcgcgac gagaaggtcg tctcgcaggc ggacctcgac
     5461 gccgccgagg ccgcgaacgt ccaggcggag gcggcgattg ccgagatcaa ggcgaccatc
     5521 ggccgcaaga cgatccgcgc cccgttctcg ggcatcctcg ggatccgcca ggtgaacctg
     5581 gggcaatacc tcaacggcgg cgacccggtc gtgtcgctgc agtccctcga tccgatctac
     5641 gtcaacttca gcgtccctca gggagatttg ccgcgcgtgc ggctcggtgg agacgtccgg
     5701 gtcacgagcg agggcctcga tgaagccgtc accgggaagg tgaccgcgat cgattcggtg
     5761 gtggacgccg cgacccgcaa cgcgcaggtg caggcgctcg tccacaaccc cgggcacaag
     5821 ctcaaggccg gcatgtacgc cagggcggag gtcacgctcc ccgatcggac gacgctcgtg
     5881 ccgctccccg cctcctcgat cctctacgcg ccatacggcg actcggtgtt catcgtcgag
     5941 gagatccagg ggaaggacgg caagcgctac aagggcgtcc gacagcaggt ggtcaagctc
     6001 ggagggtcgc gcggagacca ggtggcggtc ctcagcggcg tgaagccggg cgaggaggtc
     6061 gtctcctccg gcgtgttcaa gctgcgcaac ggcgccgcgg tcgaggtcaa caatgaggtc
     6121 cagccctcga acaacacgaa cccgaagccc gaggagtcgt gatgaagttc accgatctct
     6181 tcatccgccg ccccgtcctg gcgatcgtcg tcaacctggt catcctgatc gccggtctgc
     6241 agtcgatccg ctcgctgacg gtgcggcagt acccgcgcag cgacatcgcg accatcagcg
     6301 tcgccaccgt gtacgtcggc gccaacgccg acctcgtgcg gggcttcatc acgacgccgc
     6361 tcgaacgggt catcgccagc gccgacggca tcgactacct cgagtcgtcg agcgcccagg
     6421 ggatcagcac gattaccgtg cacctgaagc tgaactacga caccaacgcc gcgctcacgc
     6481 agatccaggc gaaggtggcc caggtgcgca accagctgcc gccggaggca gaggcgccgg
     6541 tgatcgaggt ccagaccgcc gacacgcagt tcgccgcgat gtacatcggc ttctcgtccg
     6601 agagcctcga ggcgaaccag atcaccgact acctggtccg ggcggtgcag ccgaagatct
     6661 ccgccatcag cggcgtgcag agggccgata tcctcggcaa ccgcaacttc gcgatgcgca
     6721 tctggctgaa gccggaccgg atggcgtcgt acggcctctc cgcctcggac gttcgggagg
     6781 cgctggcgaa caacaactac ctctcggcgc tggggcgcac caaggggtcg atggtgtcgg
     6841 tcaacctcgt ggccaacacc gatctgcgca ccgccgagga cttccgtcag atggtggtga
     6901 aggagaagga gggatcggtc gtccggctcg gcgagatcgc cgacgtccag ctcggggcgg
     6961 agaactacga cgaccaggtc cgcttcgacg ggcagaccgc gaccttcatg gggatctggg
     7021 tgctgccgac cgccaactcg ctcgacgtcg tcaccaaggt gcgcgcagcg ctcccggaca
     7081 tcgaggctca gctgccggcc gggatgaagg tcgccgtgcc ctatgactcg acgctctaca
     7141 tccgcaacgc catcgacgag gtgctcaaga ccctctccga gacgctgctc atcgtcatca
     7201 tcgtcatctt cctgttcctc ggctcgttcc gcgccgtgct catcccggtc atcgcgatcc
     7261 cgatctcact catcggcgcc gtcttcctga tgctggcgtt cggttcgacc atcaacctcc
     7321 tgaccctcct cgcgatcgtc ctctcggtcg gcctcgtcgt ggatgacgcc atcgtcatgg
     7381 tggagaacgt cgagcggcac ctggcgctcg gcaaaccgcc ggtccaggcg gcgctcgtcg
     7441 ccgcccgcga gctcgttggc ccgatcatcg cgatgaccgt caccctggtc gcggtgtatg
     7501 cgccgatcgg gatccaggga ggcctcaccg gggctctatt ccgcgagttc gccttcacgc
     7561 tcgccggggc ggtgctcatc tcaggtgtgg tggcgctgac cctgtcgccg atgatggcct
     7621 cccgtctcct caagccgggg gccacggaac acggtctccc cggccgcatc aatcgcggct
     7681 tcgagcggct gcagcgtttc tacacgagct ggctgtccgg aacgctggcg taccgggcgg
     7741 tggtgctgac gctctgggtg atcgtggtgg cgctcatcgc tcctttctac atgttctcgc
     7801 agaaggagct ggcgccgacg gaggaccagg gggtggtctt cggcatcgtc caggcgtccg
     7861 ccaactcgac gctcgaccag acgaagctct tcgcggacca ggcgtaccag gtctacaagt
     7921 cgttcccgga gacgaagaac accttcgaga tcaccaacgc caacggaggc ttcggcggga
     7981 tggtcgtcaa gccctggagc gaac
//
LOCUS       DASRIQ010000567        10173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_757360, whole genome shotgun sequence.
ACCESSION   DASRIQ010000567 DASRIQ010000000
VERSION     DASRIQ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10173
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757360"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFD06_14055"
     CDS             <1..242
                     /locus_tag="VFD06_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04703.1"
                     /translation="AEALDGKERKAMKWLREAFERGFDDTKHAAADADLATLRERRDF
                     KEILRSHAPPAKIDAREGETPPATRPDEPPPRLMP"
     gene            complement(281..2800)
                     /locus_tag="VFD06_14060"
     CDS             complement(281..2800)
                     /locus_tag="VFD06_14060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZN04704.1"
                     /translation="MSKSMLVNVAAEEEYRVAVVDNGVLDLFEIETLSRENIKGNIYK
                     AVVEGVNSALEASFVNYGGDRAAFLPLDEINFKIYPARFDGGGGKGRGARISRHLEKG
                     MEILVQVVRDAFANKPPTLSTYYSLPGRYLVLMPGADAAGISRKIEDDEQRARLRQVL
                     ESLQIPEGYGVIVRTAGLDTSPRELQADLDALLELWRNIERAGADAKAPSLIYQERDL
                     VIRTARDHFTTDMNEVLIDDEATFKRLRKFFEAHMPEKAEVVRLYTGDRPIFSKYNVE
                     DQIEQIYKREVNLKSGGSISIDTTEALTAVDVNSARSSRGGSSEETATRTNLEAAEEI
                     ARQLRLRDIGGLIVIDFIDMESSRHIRQVERAFAQSMTRDKARYDVTRISKLGLLEVS
                     RQRMKTTKSASSFKPCPHCSGDGMVRTPESAARAAFRKIQARVARGGLAGVKVYLPNE
                     VAVYLLNEKRDDLYRLESRQRMRIEVLPQEKMRVDQFEIEELRREEPEAVPMVTADIV
                     DQAMTEGTLATPVVGEAPPMLSAADAAARAGERAVERAGERGPDPGRMVEKRAGHPPD
                     DGSPRGPRRDEHRDARGGERPADAGRDARRRRRRRGGRGGRGGERGEQPAAQRGSEHA
                     GRGPEQGGDRPHDRGGEHGDRSRPHREHRHEQRPHRSDAVPAQARPDFSEGPDGSDAQ
                     ASDAGVSSGQEFEGGASYSGNGAQQAGVAGPQGGEPRREGERHGRRRRRRRGRGRGRG
                     GAPTETGAGAASPSSSSDGASPSSWAAEPVRREHAAPADRWSAPNAPDRYEERIARSS
                     TEPGIERSAPAADSGSASGGGEGSTGDSKPKRRWWRRGTRA"
     gene            3046..3333
                     /locus_tag="VFD06_14065"
     CDS             3046..3333
                     /locus_tag="VFD06_14065"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZN04705.1"
                     /translation="MRGPSATRRRPRRGTVREIEAPERFAFETSLPDERGTPILHVLN
                     TVTLSEDHGRTTLTLKAEVLDSTPTAAPFLEGMHAGWSQTLDRLGEHLGKE"
     gene            3390..4820
                     /locus_tag="VFD06_14070"
     CDS             3390..4820
                     /locus_tag="VFD06_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZN04706.1"
                     /translation="MTLPESIAAAPETGRGGSLLRDLADALRGTAMDYTQGSLVRAIL
                     LLSVPMILEMALESIFAVVDVFWVSSLGPSAIATVGLTESVLTLVYAVAIGLSMGTTA
                     LVARRIGEKDRRAAADAAMQAIFVGVAAAVPFSIAGLFFARDILSLMGADAWSIEHGS
                     GYTAVALGSNVVVMLIWIINAIFRGAGDAAIAMRVLWLANAINLVLDPLFIFGWGPVP
                     AFGVQGAAIATSIGRGVGVAVQLVVLFRGAKHIRITRAQVRLHTEVMGRLVRTSLGGI
                     GQFIIATSSWIGLVRIASVFGSEVVAGYTIAVRIFILTLLPAWGLSNAAATLVGQNLG
                     AKQPERAERSVWLTGWCAMAFLGFVSIAYVFLNEALMRLFTDDPAVVAAGAECLRIVA
                     YGYPIYAWGQVMPQAFNGAGDTWTPTKINFVCFWLLEIPLAWILALKLGLGQKGVWWS
                     IVVAETAMGVSAILLFRRGRWKTVQV"
     gene            4817..5092
                     /locus_tag="VFD06_14075"
     CDS             4817..5092
                     /locus_tag="VFD06_14075"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HZN04707.1"
                     /translation="MTASPLGNRVDEGHSVAEDDLGQIARRVYLHLGSAAGPSATPAW
                     FVDGGQPFANLAWWLAGGDVNGDGFSDAVLGNPNSSGDRVLLYLGIP"
     gene            complement(5119..6696)
                     /locus_tag="VFD06_14080"
     CDS             complement(5119..6696)
                     /locus_tag="VFD06_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN04708.1"
                     /translation="MRDSTAWTTRIVAGIALARLALNVAFHDRYGYFRDELYYLACSD
                     HLAWGYVDHPPLSIAILAVLRRLFGDSLHAIRFPAALAGMLTVVLAAMMARRLGGGRF
                     AQVTAALAAALSPVGLGNAGRYFSMNAFDLLLWAGGAYVLILILKEGREKLWILYGAI
                     AGLGLLNKYSMLFFGFGTLAGLLFTGRRRDLLRPWIWLGGLAAVLIVLPHALWEVRHG
                     FPTLEFMHNASTQKNAPLSAAEFLLEQPMEIGFGQTLLCLLGLGFFLFPPQPDHPGRT
                     RSTALRVFAVMYPVVVTVMIAGNAKAYYLTPIYFPYMAAGACALERLARRPVLGWSKP
                     LVVAVVVVFGFVAMPFTIPVLRVDDFIRYQRALGQTPKAEERSALADLPQYYADMFGW
                     EEMVEKVATLFRSLTPEEQRHTAIYARNYGEAAAIDFFGPRHGLPRASSGHNNYWHWG
                     PPTDDTQVILVFGRSNDVEDALADLRSPGRCGEATLGDATACQHCMPYENGRPILVCR
                     DPQFTLRQIWPGEKHFI"
     gene            6721..7995
                     /locus_tag="VFD06_14085"
     CDS             6721..7995
                     /locus_tag="VFD06_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04709.1"
                     /translation="MAIRGGRPAPTTRRMIVLGAGPVGVEAALHAALRGWDVSVLEAG
                     RPAEHLLAWGHIRMFSPWEMNCSAAGLEVLEAQGERPFDDPKHHPTGRALARDYVVPL
                     TRTPLLRGRVLSGCHVLGVTRDRTLKGDLVGDPVRGLRPFRVLTRRGGREVVFESEVV
                     IDATGTFGQPRRLGNGGLQAPGEFAAADRIDHRPVDFSERRRDFAGKSILLIGGGHSA
                     MTAAVALKSIAAESPRTRVFWAVRSDRQPYFRRIPDDPLPERDRLVVEAGAIAGGVGE
                     GFTMIPAATIEAIATSSPQARRARGNVTARRPLRVTLRTSRGERQVAVDRLLAHVGFQ
                     PDRGIYAELQIHECYASAGPMTLAAALLGAGGADCLSQPASSAEVLANPEPGFFILGA
                     KSYGRNSNFLIRTGVEQVRTLLAAIGPARLVA"
     gene            7995..8909
                     /locus_tag="VFD06_14090"
     CDS             7995..8909
                     /locus_tag="VFD06_14090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN04710.1"
                     /translation="MDSVRRPAEGPARPPESPTRPPEVPIRWLDTLWLQISGTICNLR
                     CTHCFISCAPDNHSHAMMTLEDVRRHVADAVRLGVREYYFTGGEPFMHRDLLTMIEET
                     LRAGPVTVLTNGLFLAEETCAALARLADAAAYSLDIRVSLDGWGAEDHDAIRGPGTFR
                     RAVEGIQRLHAGGVNPVITVTEAAEGVAAGEGRRRFLDLLRDWGLERPRFKILPLWRI
                     GAEAKRTAGYGPDDRLSEESVTPEGLENLQCAGGRMATSRGVYVCPLLIEEPEARMGD
                     RLDETLRPFPLGFGACVTCYVSGVTCRT"
     gene            8906..9736
                     /locus_tag="VFD06_14095"
     CDS             8906..9736
                     /locus_tag="VFD06_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZN04711.1"
                     /translation="MSGHREAHDSAPRIRCFPADLKRAAFFGGIYSNEPALLAAIADA
                     GARGAQALFCLGDLGGFGPHPERIAPILWQHDVITVQGNYEDAVGNDRADCGCGYTDP
                     RDNHFAQIAYDYTLRRTPDDQRAWFRTLPRSLRLEMEGKCLLLCHGSPRRQNEFLWES
                     TSSTPFLERMLDECGAHVLLCTHTGIPWSRVLPSGRQVINVGVLGRPANDGRPEVSYA
                     WLTGGKLEQVRITYDTGPFLLDMEREGLPPEFMETIATGWWTTCLEILPAAERKRGKY
                     "
     gene            9930..>10173
                     /locus_tag="VFD06_14100"
     CDS             9930..>10173
                     /locus_tag="VFD06_14100"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZN04712.1"
                     /translation="MKNDDVLRFAEPLPEPTFLLTADGTILEANRAAEGQLGLSREEV
                     RGRALADFIDDPQDEVARFLKTSARNTAVTPGRLNLK"
BASE COUNT         1451 a         3543 c         3616 g         1563 t
ORIGIN      
        1 gcgccgaggc gctcgacggg aaggagcgca aggcgatgaa atggctgcgc gaggcgttcg
       61 agcgcggctt cgacgacacg aaacacgcgg cggccgacgc cgacctcgcg accctccggg
      121 agcgccggga tttcaaggag atcctgcgct cgcacgcccc gcccgcgaag atcgacgcac
      181 gggaagggga gacgccgccg gccacacgac cggacgagcc gccgcctcga ctcatgccct
      241 agcgacgggc cgggagcggc gccggtcgct taccgtcagg tcaggcgcgg gtgccgcgtc
      301 gccaccagcg gcgcttcggc ttcgagtctc ccgtcgaacc ctccccgccg cccgaggcgg
      361 aaccgctgtc cgccgccggg gcggagcgct cgatccccgg ctcggtggag gaacgggcga
      421 tccgctcctc gtagcgatcc ggtgcgttgg gcgccgacca ccgatccgcc ggcgcggcgt
      481 gttcccgccg gaccggctcg gccgcccagg aggaggggga ggccccgtcc gacgaggacg
      541 agggggacgc cgcgcccgcg ccggtctcgg tcggggcgcc gccacggccg cggccgcgac
      601 cgcggcgccg gcgccgcctc cggccgtgcc gctcgccctc acgtcgcggc tctccgccct
      661 gcggaccggc gactcccgcc tgctgggcgc cgttcccgga atacgaagcc ccgccttcga
      721 actcctggcc ggaggagacg ccggcgtccg acgcctgtgc gtcgctcccg tctggcccct
      781 cggagaagtc cggtcgggcc tgcgccggca ctgcgtcgga gcgatgtggc cgctgttcat
      841 ggcggtgctc gcggtgcgga cggctgcggt cgccgtgttc tccgccccga tcgtgcgggc
      901 ggtcgccgcc ttgctcaggt ccgcgtccgg cgtgctcgga tccgcgctgg gccgccggct
      961 gttcgccgcg ctccccgccg cgaccgccgc gaccaccgcg gcggcgccgg cggcgccggg
     1021 catcgcggcc cgcgtccgcg ggacgctcgc cgccccgcgc gtcgcggtgc tcgtctctac
     1081 gaggtccgcg ggggctcccg tcgtccggcg ggtgcccggc gcgcttctcc accatccgtc
     1141 ccggatccgg gccgcgctcg ccggcccgct cgaccgcccg ctcgccggcg cgcgccgcgg
     1201 cgtcggcggc ggacagcatc ggcggcgcct ctcccacgac cggcgtcgcg agcgtccctt
     1261 cggtcatcgc ctgatcgacg atgtcggcgg tgaccatcgg caccgcctcc ggctcctcgc
     1321 gccgcagctc ctcgatctcg aactggtcca cccgcatctt ctcctgcggg agcacctcga
     1381 tgcgcatgcg ctgccggctc tcgaggcggt acaggtcgtc ccgcttctcg ttgagcaggt
     1441 agaccgccac ctcgttcggc aggtacacct tcacccccgc gaggccgccg cgcgccacgc
     1501 gcgcctgaat cttgcggaag gcggcgcgcg ccgccgactc gggggtgcgg accatcccgt
     1561 cgccggagca gtgcgggcac ggtttgaacg acgaagcgct cttcgtcgtc ttcatccgct
     1621 gccgcgacac ttcgaggagg ccgagcttgg agatgcgggt gacgtcgtag cgcgccttgt
     1681 cgcgcgtcat cgactgggcg aaggcgcgct ccacctggcg gatgtgccgg ctcgactcca
     1741 tgtcgatgaa gtcgatgacg atgagcccgc cgatgtcccg gaggcgcagc tgccgggcga
     1801 tctcctcggc cgcctcgagg ttcgtgcggg tggcggtctc ctcgctcgac ccgccgcgcg
     1861 acgagcgggc ggagttcacg tccaccgccg tcagcgcctc ggtcgtgtcg atggagatcg
     1921 acccgccgct cttgagattc acctcgcgct tgtagatctg ctcgatctgg tcctcgacgt
     1981 tgtacttcga gaagatcggc cggtcgcccg tgtagaggcg cacgacctcg gccttctccg
     2041 gcatgtgcgc ctcgaagaac ttccgcaggc gcttgaaggt cgcctcgtcg tcgatcagga
     2101 cctcgttcat gtcggtcgtg aagtggtcgc gcgccgtgcg gatgacgagg tcccgttcct
     2161 ggtagatgag ggagggggcc ttcgcgtcgg cgccggcccg ctcgatgttg cgccacagct
     2221 ccaggagcgc gtcgaggtcg gcctgcagct cgcgcgggct cgtgtcgaga ccggcggtgc
     2281 ggacgatgac gccgtagccc tccgggatct gcaggctctc gagcacctgc ctgaggcgcg
     2341 cgcgctgctc gtcgtcctcg atcttgcgtg agatgccggc ggcgtccgcc cccggcatca
     2401 ggacgaggta gcgtcccggc agggagtagt aggtgctcag cgtggggggc ttgttggcga
     2461 aggcgtcccg caccacctgg acgaggattt ccatcccctt ttccaggtgg cggctgatgc
     2521 gcgccccgcg ccccttgcct ccgccgccgt cgaagcgggc gggatagatc ttgaagttga
     2581 tctcgtcgag cggcaggaag gcggcccggt cgccgccgta gttgacgaag gaggcctcga
     2641 gggcgctgtt caccccctcg acgaccgcct tgtagatgtt gcctttgatg ttctcgcggg
     2701 aaagggtctc gatctcgaac agatcgagca ctccgttgtc gacgacggcg acgcggtatt
     2761 cctcttccgc cgcgacattg accagcatgg atttcgacat ccggtacgta tctccttggc
     2821 gcccttcggc catcctccga ctatcggaat gagggggcgg gcgcgtcacg cggggggctc
     2881 gaccgggact ccggcgccat cgggcgcctg ggcttatctc cgctcgggag cacccccgca
     2941 acggtccgga gtatagcacc ggtcccccaa gtcccctgga tagggggggc cggcaccccc
     3001 tgacccggat cgggcggtcg gggaagcggg ggcgggctat cgtggctgag gggcccaagc
     3061 gccacgcggc ggcgcccccg ccgcgggacc gtacgtgaga tcgaggcgcc ggagcggttc
     3121 gcgttcgaga cctcgctccc ggacgagcgc gggacaccca tcctgcacgt gctcaacacc
     3181 gtgaccctgt cggaggacca cggccgcacg actctcactc tgaaggccga ggtgctcgac
     3241 tcgacgccga ctgccgcccc gttcctcgaa gggatgcacg ccggctggag ccagacgctc
     3301 gaccgcctcg gagagcacct cggcaaggag taggcgcacc gacacgcgca tctgtccgga
     3361 gtaaactccg cccctcacgc aggggaacct tgacgctgcc ggaatcaatc gccgccgccc
     3421 cggaaacggg gcgcggcggg agcctcctcc gcgatctcgc cgacgccctg cgcggcacgg
     3481 cgatggacta cacgcagggg agcctcgtcc gcgccatcct gctgctctcc gtgccgatga
     3541 tcctcgagat ggcgctcgag tcgatcttcg ccgtcgtgga cgtcttctgg gtgtcgtcgc
     3601 tcggcccctc ggcgatcgcc acggtcggcc tcacggaatc ggtcctgacg ctggtgtacg
     3661 cggtggcgat cggcctgtcg atggggacga cggcgctcgt ggcccgccgc atcggcgaga
     3721 aggatcgccg cgccgccgcg gacgccgcca tgcaggcgat cttcgtcggg gtggcggcgg
     3781 ccgttccgtt cagcatcgcc ggcctgttct tcgcgcgcga catcctgagt ctcatggggg
     3841 ccgatgcctg gagcatcgag cacgggtccg gctacaccgc cgtggcgctc gggtcgaacg
     3901 tcgtggtgat gctcatctgg atcatcaacg cgatcttccg tggggccggg gacgccgcca
     3961 tcgccatgcg ggtgctgtgg ctcgccaacg ccatcaacct cgtgctcgac ccgctgttca
     4021 tcttcggctg ggggccggtc ccggccttcg gggtgcaggg ggcggcgatc gccacgagca
     4081 tcgggcgcgg tgtcggagtg gccgttcagc tcgtcgtgct gttccgcggc gccaagcaca
     4141 tccgcatcac gagagcgcag gtgcgcctgc acaccgaagt catggggcgg ctggtgcgca
     4201 cgtcgctcgg cgggatcggc cagttcatca tcgcgacgtc gtcatggatc gggctcgtgc
     4261 gcatcgcctc ggtgttcggg agcgaggtgg tggccggcta caccatcgcc gtgcgcatct
     4321 tcatcctgac gctcctgccg gcctggggcc tgtcgaacgc cgccgccacg ctcgtcgggc
     4381 agaacctcgg ggcgaagcag ccggagcggg cggaacgctc cgtctggctc accgggtggt
     4441 gcgcgatggc attcctgggc ttcgtgtcga tcgcctacgt cttcctcaac gaggcgttga
     4501 tgcggctgtt caccgacgac ccggcggtcg tcgccgccgg cgccgaatgc ctgcgcatcg
     4561 tcgcgtacgg ctaccccatc tatgcctggg ggcaggtgat gccgcaggcg ttcaacggcg
     4621 ccggcgacac ctggacgccg accaagatca acttcgtctg cttctggctg ctcgagatcc
     4681 cgctcgcctg gatcctggcc ctcaagctcg gcttgggaca gaagggggtc tggtggtcga
     4741 tcgtcgtcgc cgagaccgcg atgggcgtct ccgccatcct cctcttccgc cgcggccgct
     4801 ggaagaccgt gcaggtgtga cggcgtcgcc cctcgggaat cgcgtcgatg agggtcacag
     4861 cgttgctgaa gatgacctgg gccagatcgc gcgccgggtc tatctgcacc tggggagtgc
     4921 cgccggtcca tctgccacgc cggcgtggtt cgtggatggc gggcagccgt tcgcaaacct
     4981 cgcgtggtgg cttgccgggg gagatgtcaa cggcgatgga ttcagcgatg ccgtcctcgg
     5041 caatcccaac agcagcggcg acagggtcct tctctatctc ggaatcccgt aaaggaaccg
     5101 cgaagagaag aaccccgact agatgaagtg cttctcgccg ggccagatct gccgcagggt
     5161 gaactgcggg tcgcggcaga cgaggatggg gcggccgttc tcgtacggca tgcagtgctg
     5221 acaggcggtc gcgtcgccca gggttgcctc accgcagcgg ccgggggagc gcagatcggc
     5281 gagggcgtcc tcgacgtcgt tgctgcggcc gaagaccagg atgacctgcg tgtcgtcagt
     5341 gggagggccc cagtgccagt agttgttgtg ccccgacgaa gcgcgcggca gcccgtggcg
     5401 cgggccgaag aagtcgatcg ccgcggcctc gccatagttg cgggcgtaga tcgcggtgtg
     5461 ccgctgctcc tccggggtca gactccggaa gagggtggcc actttctcca ccatctcctc
     5521 ccagccgaac atgtcggcgt agtactgcgg caggtcggcg agcgcgctgc gctcctccgc
     5581 cttcggcgtc tggccgaggg cccgctggta gcggatgaag tcgtcgacac gcaggaccgg
     5641 gatggtgaac ggcatcgcca cgaagccgaa gacgacgacc acggcgacga cgagcggttt
     5701 ggaccagccc agaaccggcc gccgtgccag ccgctcgagc gcgcaggcgc cggccgccat
     5761 gtaagggaag tagatcggcg tgaggtagta cgccttggcg ttgccggcga tcatcaccgt
     5821 caccactacc gggtacatga ccgcgaaaac ccgcagcgcc gttgatcgcg tccggccggg
     5881 atggtccggc tgcggcggga aaaggaagaa cccgagcccg agcaggcaca gcagcgtctg
     5941 gccgaagccg atctccatcg gctgctccag gaggaactcg gcggccgaca gcggcgcgtt
     6001 cttctgcgtg ctggcgttgt gcatgaattc gagggtgggg aacccgtgcc gcacctccca
     6061 cagcgcatgc ggcaggacga tcaaaaccgc cgccagtccc ccgagccaga tccacggccg
     6121 cagcagatcg cggcgcctcc cggtgaagag cagccccgcg agcgtgccga agccgaagaa
     6181 gagcatcgag tacttgttga gcaggccgag cccggcgatg gccccgtaga gaatccagag
     6241 cttttcccgt ccttccttga ggatcaggat gaggacgtag gccccgccgg cccacagaag
     6301 caggtcgaag gcgttcatcg agaagtagcg cccggcgttg ccgaggccga ccggggagag
     6361 ggcggcggcg agcgccgccg tcacctgcgc aaagcggccg ccaccgagcc ggcgcgccat
     6421 catcgccgcc aggacgacgg tgagcatccc cgcgagcgcc gcgggaaacc ggatggcgtg
     6481 cagcgagtcg ccgaacagcc gccgcagcac ggcgagaatg gcgatggaga ggggagggtg
     6541 gtcgacgtac ccccacgcca ggtggtcgct gcacgcgagg tagtacagct cgtcgcggaa
     6601 atagccgtac cgatcgtgga acgcgacgtt gagcgcgagg cgggcgagcg cgatgcctgc
     6661 gacaatccgg gtcgtccacg cagtcgagtc gcgcatgggc gcgtatggta ccgtgacctc
     6721 atggcaatca gggggggccg tccggcgccg accacgcgcc ggatgatcgt cctcggcgcc
     6781 ggaccggtcg gtgtcgaagc ggcgcttcac gccgcgctgc gcggctggga cgtgagcgtg
     6841 ctcgaagcgg ggcggccggc ggagcacctc ctcgcctggg gccacatccg gatgttctcg
     6901 ccgtgggaga tgaactgttc cgccgcgggg ctcgaggtcc tcgaggcgca gggggagcgc
     6961 cccttcgacg acccgaagca ccatccgacc ggccgcgcgc tggcgcgcga ctacgtcgtg
     7021 ccgctgaccc gcacgccgct tttgcgcggc cgggtgctca gcggctgcca cgtcctcggc
     7081 gtgacgcgcg atcgcaccct caaaggggat ctcgtcggcg acccggtgcg cggcttgcgg
     7141 cccttccggg tgctgacgcg gcgcggcggg cgcgaggtcg tcttcgagtc ggaggtggtc
     7201 atcgacgcca ccggcacgtt cggccagccg cggcggctcg gcaacggggg ccttcaggcc
     7261 cccggtgaat tcgcggcggc cgaccggatc gaccatcggc cggtcgattt ttcggagcgc
     7321 cggcgtgact tcgcggggaa gtccatcctg ctcatcggcg gcggccacag cgccatgacc
     7381 gccgcggtgg cgctgaagtc gatcgccgcg gagagcccgc gcacgcgggt gttctgggcg
     7441 gtgcggtccg accgacagcc gtacttccgg cgcatcccgg acgacccgct gccggagcgg
     7501 gaccggctcg tcgtggaggc cggggcgatc gcggggggcg tgggggaggg gttcaccatg
     7561 atcccggcgg ccacgatcga ggcgatcgcg acgtcatcgc cgcaggcgcg ccgcgccagg
     7621 gggaacgtca ccgcgcgcag accgctgcgc gtcacgctgc gcacctccag gggagagagg
     7681 caggtcgccg tcgatcgcct cctggcgcac gtcgggttcc agccggaccg ggggatctac
     7741 gccgaacttc agatccatga gtgctacgcc tcggccgggc cgatgacgct cgcggcggcg
     7801 ctcctgggcg cgggcggcgc cgactgcctg tcgcagccgg cgtcgagcgc cgaggtgctc
     7861 gccaatcccg agccgggctt cttcatcctc ggggccaagt cgtacggccg caactcgaac
     7921 ttcctgatcc gcaccggcgt ggagcaggtc aggacgctgc tcgcggcgat cggaccggcg
     7981 cgcctggtgg cgtgatggac tcggtgcggc ggccggcgga aggtccggcc cgcccgccgg
     8041 agtctccgac ccgtccgccg gaagtcccga ttcgctggct cgacacgctc tggctgcaga
     8101 tatcaggaac catctgcaac ctgcgctgca cccactgctt catctcctgc gcgccggaca
     8161 accacagcca cgcgatgatg acgctcgagg acgtccgacg ccacgtcgcc gacgccgtgc
     8221 gcctgggggt gcgggagtac tacttcaccg gcggcgagcc gttcatgcac cgcgatctcc
     8281 tgacgatgat cgaggagacc ctgcgggcgg ggccggtgac ggtgctcacg aatggcctct
     8341 tcctggcgga ggagacctgc gcggccctcg cgcgcctggc cgacgccgcc gcatactcgc
     8401 tcgacatccg cgtctcgctc gacggatggg gggcggagga tcacgacgcc atccgcggtc
     8461 cgggcacctt ccgccgcgcc gtggagggga tccagcgtct gcacgcgggg ggagtgaacc
     8521 cggtcatcac cgtgaccgag gcggccgaag gggtcgccgc gggggagggg aggaggcgct
     8581 ttctcgacct cctgcgcgac tgggggctcg agcgcccgcg ctttaagatt ttgccgctct
     8641 ggcgcatcgg cgcggaggcg aagcggacgg cggggtacgg tccggacgat cggctctcgg
     8701 aggagtccgt gacgccggag gggctggaga acctccagtg cgccggcggc cgcatggcga
     8761 cgagccgcgg cgtgtacgtc tgtccgctgc tcatcgagga gccggaggcg cgcatggggg
     8821 acaggctcga cgagacgctg cgcccctttc cgctgggctt cggcgcctgc gtcacctgct
     8881 acgtcagcgg agtcacctgc cggacatgag cggccaccgc gaagcgcacg actctgcgcc
     8941 gcgcatccgc tgcttcccgg ccgacctgaa gcgggccgcc ttcttcggag ggatctacag
     9001 caacgaaccg gcgctgctgg cggcgatcgc cgacgcgggg gcgcgcggcg cccaggccct
     9061 cttctgcctc ggcgatctcg gcggcttcgg gccgcacccg gagcgcatcg cgccgatcct
     9121 gtggcagcac gacgtcatca ccgtgcaggg gaactacgaa gacgccgtcg gaaacgaccg
     9181 ggccgactgc ggctgcggct acaccgaccc gcgcgacaac cacttcgcac agatcgccta
     9241 cgactacacc ctgcgccgca ccccggacga tcagcgggcc tggttccgaa ccctcccgcg
     9301 gtcgctgcgt ctggagatgg aggggaaatg cctcctcctc tgccacggtt cgccgcgccg
     9361 ccagaacgag ttcctgtggg agtccacctc ctcgaccccg tttctcgagc ggatgctcga
     9421 tgagtgcggc gcccacgtcc tgctctgtac ccacaccggc atcccgtgga gccgggttct
     9481 tccgtcgggg agacaggtga tcaacgtcgg ggtgctcggc cggccggcga acgacgggcg
     9541 gcccgaggtt tcgtacgcgt ggctgacggg agggaagttg gagcaggtgc ggatcacgta
     9601 cgacacgggt cccttcctgc tcgacatgga acgcgaaggc ctgccgccgg aattcatgga
     9661 gacgatcgcc accggctggt ggaccacctg cctcgagatt cttccggcgg cggagcgcaa
     9721 gcgcgggaaa tactagggtt acaggagaag cctaggggtt tttcggtatt gttggctcca
     9781 actgctagga gtctactcta ttggcccagg tcacagattc atgacatccg aaggagcgct
     9841 ttcgtgaaca actcaggttc gcctgcgaca ggcgcgccct cgggctccct tcggcgtcgc
     9901 cgcaaggact ccggggctga ccggctgtcg tgaagaatga tgacgttctc cggttcgcgg
     9961 agccgctgcc cgagccgacc ttccttctga cggcggacgg aaccatcctc gaggccaatc
    10021 gcgccgccga gggacagctc ggactgtccc gggaggaggt ccggggtcgg gcgctcgccg
    10081 acttcatcga cgatccacag gacgaagtcg cccggttcct gaaaacatcc gcccgcaaca
    10141 ccgccgtgac ccccgggcgc ctgaacctga aac
//
LOCUS       DASRIQ010000568         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2300303, whole genome shotgun sequence.
ACCESSION   DASRIQ010000568 DASRIQ010000000
VERSION     DASRIQ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2300303"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1460
                     /locus_tag="VFD06_14105"
     CDS             162..1460
                     /locus_tag="VFD06_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04713.1"
                     /translation="MTILNARGILPAIAVFALAGCRGVGDDPCHVVLRDPGGPPEISR
                     LRAEARAATHPLPYLERLGWSFIAREAIDHDHAHFDEALAAAACMEQRAPGDVDARLL
                     RAHALVSMHRFKEGEAVARTLAGESSSWLASAVLGDALLEQGLVEEAAVHYQRMADGN
                     PGSQALSRAAHLRWLLGDRDGAIEAMAGAAAAGDPRDARAGAWYRARLAHYLLEAGRP
                     GEARRLIDGALALVSGHAPSLHARGLLLLFEGRPQEAVEPLRAAVAADPLPEYLWALQ
                     EALNESGRADEARAVEARLVGEGEREDRRGVALYLASRGVDPARALRLARAELDVRQD
                     VVTLDTVAFALLADGRIDEARDYSRRALLLGTRAPRLALHAAAIAAAAGDATEAASRA
                     GEAAAMAGALLPSERRLLERLGAARATTTTAGRPPASRTS"
     gene            1486..>2628
                     /locus_tag="VFD06_14110"
     CDS             1486..>2628
                     /locus_tag="VFD06_14110"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HZN04714.1"
                     /translation="MNRSRKTAVLAAGVLAAGLFAGQASASSHMDAPLITLDPAANTT
                     DVYAFVDQDGDDKGLVVGLSVYPFEEPGIGPNKFNFDDNVLYEIHVSLGRDLAAGEPT
                     ISYQFRFTTAFKNVNTILQSYLGVIQNVNDASQNLTQTYTVTRFDRRSGMKKVLGTGV
                     VPPNNQGIATPFYNQGNDGNNPARDGVANEADLDRYTAQSIATLAGGYRAFAGQRDDG
                     FYADVQGIFDLLQLGRRFDSQGGFNVHFMGLEIPLSDLGGDRQVVGVFATTSRPRFPI
                     LRDDDEDGADSPAIRRLRAALAAALDRGRPGGDFVQVGRQGNPLFCEGLVAIADKDRY
                     SRTRPSADAGLFRKYAESPELARRLNQFVVPGTGLVPAVETGRTDIA"
BASE COUNT          375 a          992 c          894 g          367 t
ORIGIN      
        1 acctcggtct gatcccgcac cttcgacctt caccttccac ccccacgatc ctgtaacggc
       61 gacaggccag cggctcgatc accgccatcc agagggtggc ctccgttcgt agtgaataac
      121 gggcccgccg gtgcgggtcc atttccgagg aggccaccac catgacgatt ctgaatgctc
      181 gcgggattct gccggcgatt gcggtcttcg ccctggcggg ttgcaggggg gtcggggacg
      241 acccctgcca tgtcgttctg cgcgacccgg gggggccccc ggagatctcc cggctgcgcg
      301 cagaggcgcg cgccgccacg catccgctgc cgtacctcga gcgcctcggc tggtccttca
      361 tcgcgcgcga ggcgatcgat cacgaccatg cccacttcga cgaggcgctc gcggccgccg
      421 cctgcatgga gcagcgcgcg ccgggtgacg tcgacgcacg cctcctgcgc gcccacgccc
      481 tggtgtcgat gcaccgcttc aaggaggggg aggccgtcgc gcggacgctg gcaggggagt
      541 cgtcctcgtg gctggcctcg gccgttctcg gcgacgccct cctcgagcag gggctggtcg
      601 aggaggccgc cgtccactac cagcgcatgg cggacgggaa ccccgggtcg caggccttga
      661 gccgtgcggc gcacctccga tggctgctcg gcgaccgcga cggggcgatc gaggcgatgg
      721 ccggcgccgc cgccgcggga gacccgcgcg acgcgcgcgc aggcgcctgg taccgcgccc
      781 gtctcgcgca ctatctcctc gaagccggcc gccccggcga ggcgcgccgc ctcatcgacg
      841 gcgccctggc gctcgtgtcc ggccacgcgc cgtccctgca cgcccgtggc ctgttgctgc
      901 tcttcgaggg gcgacctcag gaagcagttg agccgctgcg cgccgccgtc gcggccgacc
      961 cgctgcccga gtatctctgg gccctgcagg aggccctcaa cgagtcggga cgggccgacg
     1021 aagcccgcgc ggtcgaagcg cgcctggtcg gcgaagggga gcgcgaagac cggcgcggag
     1081 tcgccctcta cctcgcgtcg cgcggcgtcg atcccgctcg ggcgctgcgc ctggcgcgcg
     1141 ccgagctgga cgtgcgccag gatgtcgtga ccctggacac ggtcgcattc gcgctcctgg
     1201 cggacggccg catcgacgag gcgcgcgact actcgcgccg ggcgctcctc ctcgggacgc
     1261 gtgcgccgcg cctggcgttg cacgccgccg ccatcgccgc ggccgcgggg gacgccacgg
     1321 aagcggcctc ccgcgccggg gaagcggccg cgatggccgg cgcgctgctg ccgtccgagc
     1381 gccgccttct cgaacgcctg ggcgccgcgc gcgccacgac gacgaccgcg ggccgtccgc
     1441 ccgcgtcccg gacttcgtaa acaccaccga aaggagacca cgatgatgaa ccgaagcagg
     1501 aagacagcag ttctcgcggc cggcgtcctc gccgccggcc tctttgccgg gcaggcgtcg
     1561 gcgtcgagcc acatggacgc gccactcatc acgctcgacc cggcggccaa cacgaccgac
     1621 gtgtatgcct tcgtcgatca ggacggcgat gacaagggcc tcgtcgtcgg tctgtcggtc
     1681 tatcccttcg aggagcccgg gatcggcccc aacaagttca atttcgacga caacgtcctg
     1741 tacgagatcc atgtctccct cgggcgcgac ctcgccgcgg gcgagccgac catctcgtac
     1801 cagttccgct tcaccacggc gttcaagaac gtcaacacga ttctccagtc gtacctgggc
     1861 gtgatccaga acgtcaatga cgcaagccag aatctgacgc agacctacac cgtgacccgg
     1921 ttcgatcgcc gctcgggaat gaagaaggtg ctcggcacgg gcgtcgtgcc gcccaacaac
     1981 caggggatcg ccacgccctt ctacaaccag gggaacgacg gcaacaaccc ggccagggac
     2041 ggcgtggcga acgaggccga tctcgaccgt tacaccgcgc agtcgatcgc cacgctcgcc
     2101 ggcggctacc gtgccttcgc gggccagcgg gacgacggct tctacgccga cgttcaaggg
     2161 atcttcgacc tgctgcagct ggggcggcgc ttcgactccc aggggggatt caacgtccac
     2221 ttcatgggcc tcgagatccc gctgtccgac ctgggcggcg accgccaggt cgtcggcgtc
     2281 ttcgctacga ccagccgccc gcgattcccg atcctgcgcg acgacgacga ggacggcgcg
     2341 gacagcccgg ccatccggcg cctgcgcgcg gcgctcgccg cggcgctgga tcgcggccgt
     2401 cccggcggcg acttcgtcca ggtcggccgg caggggaacc cgctgttctg cgaggggctc
     2461 gtggcgatcg ccgacaagga tcgctacagc cgcacccgcc cctccgccga cgcgggcctc
     2521 tttcgcaagt acgccgagag ccccgagctg gcgcgccggc tgaaccagtt cgtcgtgccc
     2581 ggcacgggcc tcgtgcccgc cgtcgagacc ggccgcaccg acatcgcc
//
LOCUS       DASRIQ010000569         5792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3624821, whole genome shotgun sequence.
ACCESSION   DASRIQ010000569 DASRIQ010000000
VERSION     DASRIQ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3624821"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFD06_14115"
     CDS             <1..701
                     /locus_tag="VFD06_14115"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZN04715.1"
                     /translation="WADGYPFTNKPVVSDATCDNVDDDCDGSTDEDLAALNCGVGACH
                     VTTPACLNGQTQSCTPGTPTAEVCDGADNDCDGSPDEDLGVITCGLGACEATVPACVN
                     GAAGVCTPGTPGTEVCNGIDDDCDGVPDDGTQPNEATDFRFTGASAMTWTPAAGADTH
                     NVYRGEIVEGNPWAWNEVCLQTGLTAPLALDLAVPPVGSAFYYFAVGVNACGAGTQGS
                     DSAGAPRPAAPACP"
     gene            complement(708..2180)
                     /locus_tag="VFD06_14120"
     CDS             complement(708..2180)
                     /locus_tag="VFD06_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN04716.1"
                     /translation="MPVDAGRTPAAAAPGLVRALGPWDATLLVMGQVIGSAVFMVTGG
                     PDGIAALLPVPGALILGWIVGGLISFAGALVFAELGAAMPNAGGQYLFLREAYGDLVG
                     FLYGWTLFAIIHTGTLAALAVGYAEYFGAFVPALGTDRVIATLPLPAGALPISAGQLN
                     AVFVIAILSVVNYFGVKEGSLFQGVVMVMKIAAFAGFVLLGVLIGRGSLDHFTPLFES
                     AAAGAAAGGAAAAGAGAVAGSGLGFGAFVLAMVAMLWAYEGWNNITFTAGEIREPQRN
                     LPLSLVLGMSAVTVIYVVMNLLYIYAMPVPEMVTTARIGETAAGRLFGPGGARVMTLA
                     VLVSVFGCISATVISGPRVFYAMAKDGLFFRRLAEIHPRFRTPARAIVWQGVWAGLLC
                     LSGTYTRLFTFVVFAAVLFYALAGAAVIVLRRTRPGWARPYRTWGYPVTPIVYVAACT
                     VVLVNTLWSQPKESGIGLGVLALGLPAYFYWRRRRGSEAA"
     gene            complement(2171..4603)
                     /locus_tag="VFD06_14125"
     CDS             complement(2171..4603)
                     /locus_tag="VFD06_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN04717.1"
                     /translation="MSRAGHPYIYLPVAAWLLLSAFAGPSSARGADQTWERKLDPFLR
                     RVALGVERRQGPLVDRLPARSRATFNTLPPFVLAERDAADPILYVRARLDDGMTESAE
                     SPLAMPGRSAGARRRVSEGLAARFDALGVEVRGRAGAIASLAVRASALESLAAIGEVK
                     WLRAARAYRLQNDVGTSDAHTAARTENTTFGNAGAGVIVAVIDSGIDWTSPDFRNPDG
                     TTRLLGIWDQTLASGVFPPPPGFGFGSYYSRADIDAALSGGPALATQDGFGHGTHVAG
                     TAAGNGRATGNGVPEGTFAGMAPAADLLVVRVFHNDGTYCGACDLVAAVEFIDGVAQT
                     EGKPWVGNMSLGSDLGGAHDGTSPDELAIDAVVRPGRPGTQMAIAAGNFGRSDRHFHW
                     QGNLPAVGNTALTTFTLNSLTPNAGNNTDHIWLELWYRGSDSATVTVQTPGAQTVSAA
                     AGADSGIVCTTSGAVHVDATNADDPENGDNEVFITIYDSGACAPVVEPATGLWTVRVT
                     TNALGGAPGGPFDLWNAADTPRSQAGFGAGWVQLSAFNLAKSVSIPGTARHALTAGAY
                     VSKTSWVNGDGGTTTPTGTNPLGSLASFSSIGPTRDGRTKPDFAAPGQWVGSTKSKDI
                     NAAGFQMERDQVHVGFYSGTSMATPHVAGAAALLLSLHPEFDAAQVRVALQRTARADL
                     LTGGVPNNRFGWGKLRILEAAYDAAAMAAELGAGPDGQSFAWPGDDTTLSWNVYRGSL
                     PGISASSYGSCFRSGLPSPAFTDTDLPPEGEGFFYFVTGVYLHPTSGLPVEGSLGTDS
                     SGHLRPNSAACP"
     gene            4948..5388
                     /locus_tag="VFD06_14130"
     CDS             4948..5388
                     /locus_tag="VFD06_14130"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZN04718.1"
                     /translation="MLRGNSPAKIDAKGRLKIPQVFRRFMEDKYGREYFVTSINGESA
                     RIYPMPVWIEVEQKLTAAPSLHPAIVRFRNLVNYYGLPASADDQGRLLIHPLLRVKSG
                     LDGAVAVLGNMNCLDVWSRERFEAKLANEPLADDHWQMLAGLGL"
     gene            5427..>5792
                     /gene="mraW"
                     /locus_tag="VFD06_14135"
     CDS             5427..>5792
                     /gene="mraW"
                     /locus_tag="VFD06_14135"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HZN04719.1"
                     /translation="MEQPGTHRPVLLREALAALQCRPGDLVVDGTVGAGGHAEALLLA
                     TEPGGRLLGIDRDASALALARARLAQFGERVRLEQADHRALPEILDRLGLAPVDRILL
                     DLGVSSMQLDDPERGFSLRQ"
BASE COUNT          922 a         2045 c         2027 g          798 t
ORIGIN      
        1 cctgggcgga cgggtatccg ttcaccaaca agccggtggt ctccgacgcg acctgcgaca
       61 acgtcgacga cgactgcgac ggatcgaccg acgaggatct cgcggcgctg aactgcggcg
      121 tcggagcctg ccacgtgacg acgccggctt gcctcaacgg gcagacccag agctgcaccc
      181 cgggcacccc cacggccgag gtctgcgacg gcgccgacaa cgactgcgac ggatcccccg
      241 acgaagacct cggcgtgatc acatgtggtc tcggcgcctg cgaggcgacc gtccctgcct
      301 gcgtcaacgg cgcggccgga gtctgcacgc cgggaacgcc tgggacggaa gtgtgcaacg
      361 ggatcgacga cgactgcgac ggcgtgcccg acgacggcac tcagccaaac gaggcgacgg
      421 acttccggtt cacgggagcg tcggccatga cctggacccc tgcggccggc gctgacacgc
      481 acaatgtcta ccgcggcgag atcgtggaag ggaacccgtg ggcctggaac gaagtctgcc
      541 tgcagacggg cctcaccgcc cccctggccc tggatctcgc ggtgccgccg gttggcagcg
      601 cgttctatta cttcgcggtc ggcgtgaatg cgtgcggggc cgggactcag ggaagtgact
      661 ccgccggcgc gccgcggccc gccgcccccg cctgtccctg atccggttta ggcggcctcg
      721 gatcctcgcc ggcgccgcca gtagaaatac gccggcagcc ccagcgccag cacgccgagg
      781 ccgatgccgg actccttcgg ctggctccag agcgtattca cgaggaccac ggtgcacgcc
      841 gcgacgtaga cgatcggcgt caccgggtag ccccacgtcc ggtacgggcg cgcccacccg
      901 ggacgggtcc gacgcaggac gatgaccgcc gcccccgcca gcgcatagaa gaggaccgcg
      961 gcgaacacga cgaaagtgaa cagccgcgtg tacgtccccg acaggcacag caggccggcc
     1021 cacactccct gccagacgat ggcgcgcgcc ggcgtgcgga agcgcgggtg gatctccgcc
     1081 aggcggcgga agaacaggcc gtctttggcc atcgcgtaga agacgcgcgg tccggagatc
     1141 accgtcgcgg agatgcagcc gaacaccgac accagcacgg cgagcgtcat gacgcgcgcc
     1201 ccccccggcc cgaagaggcg cccggccgcc gtctcgccga tccgcgccgt cgtcaccatc
     1261 tccggcaccg gcatggcgta gatgtagagc aggttcatca ccacgtagat gaccgtgacg
     1321 gccgacatgc cgagcaccag cgacagcggc aggttgcgct gcggctcgcg gatctcgccg
     1381 gccgtgaagg tgatgttgtt ccacccttcg tacgcccaga gcatggcgac catcgccagg
     1441 acgaacgcgc cgaagccgag accgcttccc gcgactgcac ccgctcccgc tgcggcggcg
     1501 cccccagccg cggcacccgc cgcggcggat tcgaagagcg gcgtgaaatg atcgagggag
     1561 ccacgcccga tgaggacgcc gagcaggacg aatcccgcga aggcggcgat cttcatcacc
     1621 atcacgaccc cctggaacag gctcccctcc ttcaccccga agtagttgac gaccgagagg
     1681 atcgcaatca cgaagaccgc gttcagctgg cccgccgaga tcggcagcgc gcccgcgggg
     1741 agaggcagcg tggcgatgac gcgatcggtc ccgagcgccg gcacgaaggc gccgaagtac
     1801 tcggcgtagc cgaccgcgag cgccgcgagc gtgccggtgt ggatgatcgc gaacagcgtc
     1861 cagccgtaca ggaacccgac gagatcgccg taggcctcgc gcaggaagag gtactggccg
     1921 ccggcgttcg gcatggcggc gccgagctcg gcgaacacga gcgccccggc gaaggagatg
     1981 agcccgccga cgatccagcc gaggatcagc gcgccgggga ccggcaggag cgccgcgatc
     2041 ccgtccgggc cgccggtcac catgaagacc gccgacccga tgacctggcc catcaccagc
     2101 agggtcgcgt cccacggccc gagggcgcgc accaaccccg gcgcggcggc ggccggggtg
     2161 cggcccgcgt ctacgggcac gcggcgctgt tgggacggag atggccgctg ctgtcggtgc
     2221 cgaggctccc ctcgaccggc aggccgctcg tgggatgcag gtacacgccg gtcacgaagt
     2281 agaagaaccc ttctccctcg ggcgggagat cggtatcggt gaaggcgggg gacggaaggc
     2341 cgctccgaaa gcaggaaccg tagctgctcg ccgagatccc cggcagggat ccgcggtaca
     2401 cgttccacga cagggtcgtg tcatcccccg gccaggcgaa cgactggcca tccggcccgg
     2461 cgcccagctc tgccgccatc gccgccgcat catacgcggc ctcgaggatg cggagcttcc
     2521 cccagccgaa gcggttgttc ggcacgccac cggtcaggag atcggcgcgc gccgtgcgct
     2581 gcagcgccac ccgcacctgg gccgcgtcga actccgggtg caggctcagg agcaaggcgg
     2641 cggcgccggc cacgtgcggc gtcgccatgc tggtgccgct gtagaaaccg acgtgcactt
     2701 gatccctttc catctggaag ccggcggcgt tgatgtcctt cgacttggtg gacccgaccc
     2761 attgccccgg agccgcgaaa tctggcttgg tgcgtccgtc gcgggtcggg ccaatgctgg
     2821 agaagctcgc gagagagccg agcggattcg ttccggtcgg ggtggtcgtc ccgccatcgc
     2881 cattcaccca cgatgtcttg ctgacgtacg cgccggcggt cagcgcgtgg cgcgcggtcc
     2941 caggaatcga gacgctcttg gcgagattga acgcgctgag ctgcacccat ccggccccga
     3001 atccggcctg gctgcgcggc gtgtcggccg cgttccacag gtcgaacggt ccgccgggcg
     3061 cgcctcccag ggcgttcgtg gtcacgcgca cggtccacag cccggtcgcc ggctccacca
     3121 ccggcgcgca ggcgccgctg tcgtagatgg tgatgaacac ctcgttgtcg ccgttctccg
     3181 gatcgtcggc gttcgtcgcg tccacgtgca ccgcccccga cgtcgtgcag acgatgccgc
     3241 tgtcggcgcc ggccgcggcg ctgacggtct gcgcccccgg cgtctgcacc gtcacggtgg
     3301 cgctgtccga tccgcggtac cagagctcga gccagatgtg gtcggtgttg ttgccggcgt
     3361 tcggggtgag cgagttgagc gtgaaggtcg tcaaagcggt gttgcccacg gcggggaggt
     3421 tcccctgcca gtggaagtgc cgatcgctcc ttccgaaatt gccggcagcg atcgccatct
     3481 gtgtccccgg gcgccccggc cgcaccaccg cgtcgatcgc cagctcgtcg gggctcgtgc
     3541 cgtcgtgggc gccgccgagg tcggagccga ggctcatgtt gccgacccac ggcttcccct
     3601 cggtctgggc gacgccgtcg atgaactcga ccgccgcgac gagatcgcac gcgccgcagt
     3661 acgtgccgtc gttgtggaac acccgcacca cgaggagatc cgccgcgggc gccatgccgg
     3721 cgaacgtccc ctccggcacg ccattgccgg tggcgcggcc gttgcccgcg gcagtgcccg
     3781 cgacgtgcgt gccgtgtccg aacccgtcct gcgtcgccag cgccggcccg ccggagagcg
     3841 ccgcgtcgat gtcggcgcgg ctgtagtacg agccgaagcc gaacccgggc ggcggcggaa
     3901 agacgccgct cgcgagcgtc tgatcccaga tgccgagcag gcgcgtcgtg ccgtcgggat
     3961 tgcggaaatc ggggctcgtc cagtcgatgc cgctgtcgat gaccgcgacg atcacccccg
     4021 ctccggcgtt cccgaacgtg gtgttctcgg tgcgcgccgc ggtgtgggca tcggaggtgc
     4081 cgacgtcgtt ctggaggcgg taggcgcgcg ccgcacgcaa ccacttcact tcgccgatcg
     4141 ccgcgagcga ctcgagggcc gaggcgcgca ccgcgagaga cgcgatcgct ccggcgcggc
     4201 cgcgcacctc gacgccgagc gcgtcgaagc gggccgcgag tccctcggac acgcggcgcc
     4261 gcgcgcccgc ggagcggccg ggcatggcga gggggctctc cgcggattcc gtcatgccgt
     4321 cgtcgagacg cgcccggacg tacaggatcg gatccgcggc gtcccgctcc gccaacacga
     4381 acggtggcag cgtgttgaag gtcgcgcggg agcgcgccgg caggcgatcg accagaggtc
     4441 cctgccgccg ctcgacgccg agcgcgacgc ggcgcaggaa cggatcgagc ttgcgttccc
     4501 aggtctgatc ggcgccccgg gcggacgacg ggcccgcgaa cgccgacagc agaagccagg
     4561 cggccacggg aaggtagata taaggatggc cggcccggga catcgtcgaa tggtacggcg
     4621 aagagcgacc tcatgcaacg gctggaggcc tgtccggatg acgcgcctcc tccagccggc
     4681 cgattggatc cgcgtaccaa caaattggga tatgccgagg ccggcgtcac aagcgatcgc
     4741 ctgttttcgg cggacttccc cctgcgctat ctccttctga ttgttcgggt ttggagcaaa
     4801 gaaaagagat tttcctcctt gacagccccg gaggggcttc gtatagtccg cagtggagtg
     4861 gggtggagca acgtgggtgg ctcgcccctg accctcgaca ttcaaacgat tggcggcgtg
     4921 cgaggtccct tggggggttg aggaccgatg ctgaggggca atagtccggc caaaatcgac
     4981 gccaagggac gtctcaaaat cccccaggtc ttccgacgct tcatggaaga caagtacggc
     5041 cgcgagtact tcgtcacctc gatcaacggc gagagcgccc gcatctaccc gatgccggtg
     5101 tggatcgaag tggagcagaa gctcaccgcg gcgccgtcgc tgcaccccgc catcgtccgg
     5161 ttccgcaacc tggtgaacta ctacggcctg ccggcgtccg ccgacgatca ggggcgcctg
     5221 ctgatccacc cgctgctgcg cgtcaagagc ggtctcgacg gcgcggtggc ggtgctcggc
     5281 aacatgaact gtctcgatgt ctggagccgc gagcgcttcg aggcgaagct cgccaacgag
     5341 ccgctcgcgg acgatcactg gcagatgctc gcgggcctcg gcttgtagtc aggaaccgca
     5401 gggagcacag tgaaacccgg aacacgatgg agcagcctgg gacgcaccgg ccggtcctgc
     5461 tgcgggaggc gctcgcggcc ctccagtgcc ggccgggcga cctggtcgtg gacggcaccg
     5521 tgggggccgg cgggcacgcc gaggccctgc tgctcgcgac cgagccgggc gggcgtctcc
     5581 tcgggatcga tcgcgacgcc tccgccctcg ccctggcgcg cgcacgcctc gcgcagttcg
     5641 gcgaaagggt ccgcctcgaa caggcggatc accgcgcgct gccggagatc ctcgatcgcc
     5701 tcgggctggc gccggtggac cgcatcctcc tcgatctcgg ggtctcctcg atgcagctcg
     5761 acgatccgga gcgcggcttc tcgctgcgcc ag
//
LOCUS       DASRIQ010000570         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3625820, whole genome shotgun sequence.
ACCESSION   DASRIQ010000570 DASRIQ010000000
VERSION     DASRIQ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625820"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2210)
                     /locus_tag="VFD06_14140"
     CDS             complement(<1..>2210)
                     /locus_tag="VFD06_14140"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZN04720.1"
                     /translation="WAPFRHPHSIATNVNANPAWEYKTGGICGFQTAGGLNKFGIWHT
                     GDGDPNTPSGAAVACDNHAKPSDPGTPAKIETIFDVAESAIVAKVNQAVDARGFPWNV
                     EFQRLGFNLNIQLYDGYAGGGVNIDNDIDNDGANSLLAQNTDLYYERRAGGWPNGVFR
                     FNGEYFPGNGIDPAVIVPRQHTFGPFQNPNGSFNLDGDESGFTGATQNTNPDSTSPIP
                     TAPPDLLPFPQPLAVLAGTCTGGAAAGAPCAPGAPGDPCVVQGGVCTAQTNTIAGPVR
                     NFDATLVGFEGGFARLNNLAAIENFMFFLPGPAGNRWQIGIGFWAAEGTPNVADYGVS
                     IDDVVFEWNEWHPVDESAVPAPPACQRFGGPGQPAGGACATVTADRTTLYECEESIEI
                     TVRDAKCTALGPGATTTLGGPCTTHAPCGAGGSCTAALAAVEVAVVTDSDANVVTVSG
                     QPILTPTAKRFTLPAVAGQPGLFRGRVPFTTLAAGPGSVHANPPTDGRFIVYYFDPLC
                     DGDRDGQAGEDGFDNTDGDGIPDAADKCPEVYNPLQQDADGDGLGDLCDNCPNAANAT
                     QADANVDGVGDACEFEDIDGDAVPNAEDNCPDVRNPNQSDLEFDGRGDLCDTLKTSGI
                     TFGTSAGAAECVNGTCTKPAAAVGQTCVMDEDCIRSCNAGVCSDTGGYVSPTPRVGQA
                     CSTHAQCYVDVDRDADGVIDANDNCVLARNGPLGGPNNQIDSDGDGLGDACDGD"
BASE COUNT          294 a          756 c          779 g          381 t
ORIGIN      
        1 gtcgccgtcg caggcatccc cgagtccatc gccgtcggag tcgatctggt tgttcggccc
       61 gccgagtggt ccgtttctgg cgaggacgca gttgtcgttc gcgtcgatca caccgtcggc
      121 gtcgcgatcg acgtccacat agcactgcgc gtgggtcgag cacgcctggc cgaccctcgg
      181 ggtcggggag acgtaacccc ccgtgtcgga gcagacaccg gcgttgcagg aacggatgca
      241 gtcctcgtcc atcacgcacg tctgcccgac ggcggcggcc ggcttggtac aggtcccgtt
      301 cacgcactcg gcggcgccgg cgctcgttcc aaaggtaata cccgaagtct tcagcgtgtc
      361 gcagaggtcg ccccggccgt cgaactcgag gtcggactgg ttcggattgc ggacatccgg
      421 gcagttgtcc tcggcgttcg gaaccgcgtc gccgtcgatg tcttcgaatt cgcaggcgtc
      481 gccgacgccg tcgacattgg cgtcggcctg ggtcgcgttg gccgcgttcg gacagttgtc
      541 gcacaggtcg ccgaggccgt cgccgtcggc gtcctgctgc agcgggttgt agacctcagg
      601 acatttgtcg gcggcatccg ggatcccgtc gccgtcggtg ttgtcgaagc cgtcctcccc
      661 cgcctggccg tcccggtcgc cgtcgcacaa cggatcgaag taatagacga tgaagcgccc
      721 gtcggtcggc gggttggcgt gcacgcttcc cgggcccgcc gcgagcgtgg tgaacggcac
      781 gcgcccacgg aacagccccg gctgcccggc gaccgcgggc agcgtgaagc gcttcgcggt
      841 cggtgtcagg atcggctggc cggagaccgt cacgacgttc gcgtcgctgt cggtgacgac
      901 cgccacctcg accgcggcga gcgcggcggt gcacgacccg cccgcgccgc acggcgcgtg
      961 cgtggtgcag gggccgccga gggtggtcgt cgcccccggc cccagcgccg tgcacttggc
     1021 gtcgcggacg gtgatctcga tcgactcttc gcactcgtac agggtcgtcc gatcggccgt
     1081 gaccgtggcg caggcgccgc ccgccggctg ccccggaccg ccgaagcgct ggcaggccgg
     1141 cggcgccggg acggcggact cgtccaccgg gtgccattcg ttccactcga agaccacgtc
     1201 gtcgatcgac acgccgtagt cggcgacatt cggagtgccc tccgcggccc agaagccgat
     1261 gccgatctgc cagcggttgc ccgccgggcc gggcaggaag aacatgaagt tctcgatggc
     1321 tgcgaggttg ttcagcctcg cgaacccccc ctcgaagccg acgagcgtcg cgtcgaagtt
     1381 ccggaccggg ccggcgatgg tgttcgtctg cgcggtgcag acacccccct ggacgacgca
     1441 gggatcgccg ggggcaccgg gggcgcacgg cgctccggcg gcggcgccac cggtgcaagt
     1501 ccccgccagg accgccaggg gctgcgggaa cggcagcagg tcgggagggg cggtcgggat
     1561 cgggctcgtg ctgtccggat tcgtgttctg agtcgccccg gtgaaaccgc tctcgtctcc
     1621 gtccagattg aacgacccgt tcggattctg gaacgggccg aaggtgtgct gtctcgggac
     1681 gatcacggct ggatcgatgc cgttccccgg gaagtactcc ccattgaatc ggaacacgcc
     1741 gttcggccac ccgcccgcgc ggcgttcgta atagaggtcg gtgttctgcg ccagcaggct
     1801 gttggccccg tcgttgtcga tgtcgttgtc gatgttcact ccaccgccgg cgtagccgtc
     1861 gtacagctgg atgttcaggt tgaacccgag gcgctggaac tccacgttcc aggggaagcc
     1921 gcgcgcgtcc accgcctggt tgaccttggc gacgatcgcc gattcggcga cgtcgaagat
     1981 cgtctcgatc ttcgccggcg tgcccggatc gctgggtttg gcgtggttgt cgcaagcgac
     2041 cgccgcacca ctcggggtgt tcgggtcgcc gtcccccgtg tgccagatgc cgaacttgtt
     2101 cagcccgccc gccgtctgga agccgcagat cccgccggtc ttgtactccc acgccgggtt
     2161 ggcgttgacg ttcgtcgcga tgctgtgggg gtgccggaac ggcgcccagc
//
LOCUS       DASRIQ010000571         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2046173, whole genome shotgun sequence.
ACCESSION   DASRIQ010000571 DASRIQ010000000
VERSION     DASRIQ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046173"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFD06_14145"
     CDS             complement(<1..731)
                     /locus_tag="VFD06_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04721.1"
                     /translation="MRLAARDGADRTLFETVAAATATPGLAVAPLHPLEKGGVRWRSL
                     AILPADGAASPTMPSGWGVTTVASIARERDQVTLEVPGLPACVRGAPTTGLAKSALGG
                     LSTGAAVLAARNRDGFRNRIVGGRVERIVELPGERRLLLVRLLDERGADPGLLFDPAG
                     SLLGAVLPVSAGEDPALATVVLEPEPIAAAAASAPSQGARDAIPVSPTPSVLGTIPGI
                     VSQALRLSGPSRYERGLALLDEAAR"
     gene            1108..>2105
                     /locus_tag="VFD06_14150"
     CDS             1108..>2105
                     /locus_tag="VFD06_14150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZN04722.1"
                     /translation="MTRRRAVITGLGAVTPFGEGIEAFRIGLLEGKSAVRRVSLFDPG
                     DLPSQVAAEVPGFDPNLWLDPADRLRVGRIVPLALAAAREALVASGLASPDRAATMPR
                     RADVGVVIGSGAGAIDFAERQYEAYYRDGIHRVNPFAISSSVLGMLSSELSIAFGFTG
                     LSHVISDGCTSSTDAIGSAQRIIERGDLPVVLTGGAEACITRGMMEGFCRMKAAATGW
                     NDRPEKASRPFNRDRSGFVLGEGAWLLVLEEAEHARARGAAILGEVAGYGATCDAHHR
                     VHMHGEGEESTRAMALALEDAGAVPEDVDYINLHGTSTQQNDRIETRAVRNLLGARA"
BASE COUNT          291 a          752 c          793 g          269 t
ORIGIN      
        1 tgccgcgccg cctcgtcgag cagggccagc ccccgctcat agcgggacgg gccggagagg
       61 cgcagcgcct ggctgacgat cccgggaatc gtgccgagca ccgaaggggt gggggagacc
      121 gggatggcat cgcgggcccc ctgggacggg gcggaggcgg ccgcggcggc gatcggctcc
      181 ggctcgagca cgaccgtggc gagcgcgggg tcctccccgg ccgagacggg gaggacggcg
      241 ccgaggagcg atcccgccgg atcgaacagg agccccgggt cggcgccgcg ctcgtcgagg
      301 aggcgcacca ggagcagtcg ccgctccccg ggcagttcga cgatccgctc cacccgacct
      361 ccgacgatcc ggttgcggaa accgtcccgg ttgcgcgccg cgaggaccgc ggcgccggtc
      421 gacagaccac ccagggcgct cttcgccagg ccggtggtgg gagcgccgcg gacgcaggcg
      481 ggaagccccg gcacttcgag cgtcacctga tcccgctcgc gggcgatcga cgcgaccgtg
      541 gtcacgcccc agccggatgg catcgtcggc gacgcggcgc cgtcggcggg aaggatcgcg
      601 aggctgcgcc agcgcacgcc ccccttttcg agcgggtgga gcggagccac ggcgagtccc
      661 ggcgtcgccg tggccgcggc gaccgtctcg aacagcgtcc ggtccgctcc atcacgggct
      721 gcgagtcgca cgaggcacgc aggagggacg gaggcggcgg cggaaaggat ggaagtcgcc
      781 gtggcgtccg tgccgttcga ggccaggctt cgggaggagg ccgaggcgag caggaacgcg
      841 acgagcggga caggccatcg gcgaagccgt cccgtcctcc ctcccgttcc cggtcccccc
      901 agccgagaca cgcctctctc cctggccgca cgtcccttcg ccggatcccc gtccgcggcg
      961 ggcgtgcgac tgtacctgac gtcctgtgcg ggtttcgcgg cttccatgta cgccgagcgg
     1021 agcggggcgt caagacgttt cgtgcgttcc ggcaccgacc ttcccgtcgc cggccgccgt
     1081 gaccggatgc tagactcgcc ggacaccatg acacgacggc gcgcggtgat caccgggctg
     1141 ggcgcggtga cgccgttcgg ggaggggatc gaggcgttcc ggatcggcct cctcgaaggg
     1201 aaaagcgcgg tccgccgcgt ctccctcttc gaccccggcg acctgcccag ccaggtggcg
     1261 gcggaagtcc cgggcttcga cccgaatctc tggctcgatc ccgccgatcg tctccgcgtc
     1321 ggccggatcg tcccgctcgc gctcgcggcg gcccgcgagg cgctcgtcgc gtcggggctc
     1381 gcgtccccgg atcgcgccgc caccatgccc cggcgcgccg acgtgggggt cgtcatcggc
     1441 agcggcgcgg gggcgatcga tttcgccgag cggcagtacg aggcgtacta ccgggacggc
     1501 atccaccggg tcaatccgtt cgccatctct tcttcggtcc tgggcatgct gtcgagcgaa
     1561 ttgtcgatcg ccttcggctt caccggactg tcgcacgtca tcagcgacgg ctgcaccagc
     1621 agcacggacg ccatcggctc ggcgcagcgg atcatcgagc ggggggacct gccggtcgtc
     1681 ctgaccggcg gcgccgaggc ctgcattacg cgcgggatga tggaagggtt ctgccgcatg
     1741 aaggcggccg cgaccggctg gaacgatcgg ccggaaaaag cgtcccgccc gttcaaccgc
     1801 gatcgcagcg gcttcgtcct cggcgagggg gcgtggctcc tggtgctgga ggaggcggag
     1861 cacgcgcggg cgcgcggcgc cgccatcctc ggggaggtgg ccggctatgg cgccacctgc
     1921 gacgcgcacc atcgcgtcca catgcacggg gagggggagg agtcgacccg cgccatggcg
     1981 ctggcgctcg aggacgccgg ggcggtcccg gaggacgtcg actacatcaa cctgcacggc
     2041 acctcgacgc agcagaacga tcggatcgag acgcgcgccg tgaggaacct cctcggcgcg
     2101 cgcgc
//
LOCUS       DASRIQ010000572         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3626946, whole genome shotgun sequence.
ACCESSION   DASRIQ010000572 DASRIQ010000000
VERSION     DASRIQ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3626946"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFD06_14155"
     CDS             <1..827
                     /locus_tag="VFD06_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04723.1"
                     /translation="RGSAIDRQPVFSPDGEWVMFSSNRSGNLEVWKASTTTGAIRRLT
                     DDPAQDWDPAFTPDGKGMLWSSSRTGHFEIWIGRTDGTGARQLTRDGVDAENPTMTPD
                     GKWVIYNSSNPAQSGLWKIRADGTEATRIVPGAWSTPEVSPDGEWVAFRTQVLPRVLN
                     LARISDGRLEPMRIRPSGQNTTGRPRWTPDGRALAWNTYNEAGESGVLVQEFIPGADT
                     SARRRPFAGFDRGQEVDSFAISRDGLHLVQAISDGLDSLLLAEGVPGVEPPRPTRP"
     gene            980..4207
                     /locus_tag="VFD06_14160"
     CDS             980..4207
                     /locus_tag="VFD06_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HZN04724.1"
                     /translation="MARRALAVAESDTGQGDAASAIDRLVDVLARSGRGAEKETRALA
                     ERAVALRRSAGGPDDAALATSLVNLGSVLAAAAEFRAAQEALEQALSLRTAAAGPDAA
                     GTAAVKERLGALLANSGKSAGALVLLEEAAGSLADPTGDKDAELATVLTTLGGVLTNR
                     GILPAARDALDRALAIRIRLYGDDHPEVAATLFRQAFLKRKEGEAAPALALVERALAI
                     REARLPKGSLDIASALGLIAELRFDRGDLPGARASAERALALRRAALSPGHPLLATGM
                     KTLAGVLWEQGHYAEARPLFEQALAIERAALGPAHPQVALSLTNLGNILSLVGDDAAA
                     LESYGQARAIADRAMTPAQRAMLLTETAVALNALGRGDEARRNLEEALAGVEADHWQA
                     GWILYILGLVEETRGDRAAADAAAARARATLSAALGEDHVKLGLVTLLQARLRQAEGR
                     ANEADALFAQALHVLERSVGPDHLEVSNTLSDWSEARWQAGRPDEALEHALRAARIRR
                     EILAASGGAFSEREALRFRRSRGPAIDLALSRLASGGAPREAIARTCDEVIRSRAQVL
                     DEVAWRQRLARESTSERAGALVAELDQARTRLSRLTLAVYGSGGGQTDRSALDRARLE
                     REEAERALARWSASAGLPRARRDVGLAEVRAALPERSALIGFVRYARALPPSAEGSSA
                     PEPPRPEYLAFVLRPETDDIGLVALGPADRIEALVRDWRREAGHPPDGREAMGRYRAA
                     ARGLAQAVWTPLQRPLADRDLVLIVPDGALQFVSWPSLPSSAGAYLVESGPTIHLLSA
                     ERGVALDGQAPAGRGLLAIGGPDFGAAPASSPCAGSSIGALTALPSAAEEARDVGALW
                     KREEGAGSRETGDVSIFTGRGASEAAFRSSAPGRRVLHLATHGFYLADRCDPDSRGGA
                     GRAATVSDAAEAGSIDTALARTGLAFAGANRAWGDRGADGGADGLLTAQEIAALDLRG
                     VEWAVLSACGSSLGQAEAGEGILGLRRTFEIAGARSLVTSLWDLEDHSARRWIRELYR
                     ARLHGASTAGAVRQASRSILESRRRAGLADHPYYWAPFVAAGDWR"
     gene            complement(4214..5002)
                     /locus_tag="VFD06_14165"
     CDS             complement(4214..5002)
                     /locus_tag="VFD06_14165"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN04725.1"
                     /translation="MKSACRSAVRELLPWYLNGSLEGSEAGEVQAHLASCPDCVRDLH
                     DLGEIAELVRLHGAPSNAAAPEARPAAIPFPAQPKPQPGRRRRTALAAGIAAMLAGVL
                     LAALLRSERPDPRSASGDAAPAPGPAAPAGPPPAAPAMLARLDLGPGPVRGAEETPVL
                     RLPEGAERIEVSFVIPGVGRAATVRMEDEEGRPIADAVPVGGHDEMGRSTAVFSAAPF
                     RHAGSYTLVLSQVEQAEGETGAGTRIEYRYPFRFQPEANGSARP"
     gene            complement(4999..>5258)
                     /locus_tag="VFD06_14170"
     CDS             complement(4999..>5258)
                     /locus_tag="VFD06_14170"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZN04726.1"
                     /translation="LPVDEKESPASRSWAARQMLARLMDGLTEEDRLMITLVFRDYKS
                     YAEVAALLGIPEGTVKSRMARLRDRLLERRRALLAEAGERP"
BASE COUNT          664 a         1917 c         2026 g          651 t
ORIGIN      
        1 cccgcggcag cgccatcgac cggcagccgg tcttctctcc ggacggcgag tgggtgatgt
       61 tctcctccaa ccgcagcggc aacctcgagg tctggaaggc ctccacgacc accggtgcga
      121 tccgccgcct gaccgacgac ccggcgcagg actgggaccc cgccttcacc cccgacggca
      181 aagggatgct gtggtcgtcg agccgcaccg ggcacttcga gatctggatc ggccgtaccg
      241 acggcaccgg cgctcgccag ctcacgcgcg atggcgtgga cgccgagaac ccgacgatga
      301 cgccggacgg gaagtgggtc atttacaact cgagcaaccc ggcgcagagc ggcctctgga
      361 agatccgcgc cgacggcacg gaggcgacgc gcatcgtccc cggcgcgtgg agcactcccg
      421 aggtgtcgcc ggacggcgag tgggtggcct tccgcaccca ggtgctgccg cgcgtgctca
      481 acctcgcacg gatctccgac ggccgcctcg agccgatgcg tatccgtccg tcggggcaga
      541 acaccaccgg aaggccgcgc tggacgccgg acggcagagc cctcgcctgg aacacgtaca
      601 acgaggccgg cgagagcggc gtcctggtgc aggagttcat cccgggagcg gacacgagcg
      661 cccggcgccg gcccttcgcc gggttcgatc gcggccagga ggtggacagc ttcgcgatct
      721 cgcgcgacgg ccttcacctg gtccaggcga tcagcgacgg gctcgacagc ctgctgctgg
      781 ccgaaggggt gcccggcgtc gagccgcccc ggccgacccg gccttgacgg ggcggggcac
      841 ggcccggcgc atcgccgcct gcttcatcct tgcgctgaca ctcgccggca ctcggccggg
      901 gcgggccgat gcccccgccg ccggtgcgga gacgcccgag gccttgctcc gccaggggcg
      961 gtacgttgag gccgaggcgg tggcgcgacg cgcgctcgcg gtggcggagt ccgacaccgg
     1021 ccagggcgac gccgcgagcg ccatcgatcg cctggtcgac gtcctggcgc gcagtggccg
     1081 cggggctgag aaagagacgc gcgctctggc cgagcgggcc gtcgccctgc gccgctcggc
     1141 gggcggcccg gacgacgcgg cgctggcgac cagcctggtc aacctcggaa gcgtcctcgc
     1201 ggccgccgcg gagttccgcg ccgcgcagga ggcgttggag caggcgctgt cgctgcggac
     1261 cgcggcggcc ggtcccgacg cggccggcac ggcggcggtg aaggagcggc tgggggcgct
     1321 tctggccaac tccgggaagt ccgccggcgc cctcgtcctg ctcgaggagg cggcggggtc
     1381 gctcgcggat ccgacgggag acaaggacgc ggagctggcg acggtcctca ccacgctcgg
     1441 cggggtgctg acgaaccgcg ggatcctccc ggccgctcgc gacgcgctcg atcgcgcgct
     1501 cgcgattcgg atccggctgt acggcgacga ccaccccgag gtggccgcca ccctcttccg
     1561 tcaggcgttc ctgaagcgca aggaggggga ggcggcgccg gccctcgcgc tcgtggagcg
     1621 cgccctcgcc atccgcgagg cgcggctccc gaagggaagc ctcgatatcg cctcggcgct
     1681 tggtctgatc gccgagctgc gcttcgatcg tggcgatctc cccggagcgc gggcgagcgc
     1741 cgagcgcgcc ctcgcgctgc gccgcgccgc gctctccccg gggcacccgc tgctcgccac
     1801 cgggatgaag acgctcgcag gcgtcctgtg ggagcaggga cactacgccg aggcgcggcc
     1861 gctcttcgag caggcgctcg ccatcgagcg cgcggcgctg ggcccggcgc atcctcaagt
     1921 cgcattgagc ctcaccaacc tcggcaacat cctgagcctc gtcggagacg acgcggcggc
     1981 gctcgaatcc tatggacagg cgcgggccat cgccgatcgg gcgatgaccc cggcgcagcg
     2041 cgccatgctc ctgacggaga cggcggtcgc gctcaacgcg ctggggcgcg gcgacgaggc
     2101 gcggcgcaac ctcgaggagg cgctcgccgg ggtcgaggcg gatcactggc aggccggctg
     2161 gatcctctac atcctcggcc tcgtcgaaga aacgcgcggc gatcgcgcgg ccgcagacgc
     2221 ggcggcggcg cgggcgcgcg cgacgctctc ggccgccctc ggagaagacc acgtcaagct
     2281 ggggctggtg accctgctgc aggcacgcct gcgccaggcg gagggccggg cgaacgaggc
     2341 ggacgcgctc ttcgcgcagg ccctccatgt cttggagcga agcgtggggc ccgatcatct
     2401 ggaagtctcc aacacgcttt ccgactggag cgaggcgcgc tggcaggccg ggaggccgga
     2461 cgaggcgctc gagcacgcgc tgcgcgccgc gcgcatccgg cgcgagatcc tggcggcgtc
     2521 cggaggcgcc ttctccgagc gcgaagcgct gcgcttcagg cgaagccgcg gccccgcgat
     2581 cgacctcgcc ctctcccgcc tcgcgtccgg aggcgcgccg cgtgaggcga tcgcccggac
     2641 ctgcgacgag gtgatccgct cccgggcgca ggtgctcgac gaagtggcct ggcggcagcg
     2701 cctggcgcgc gaatcgactt cggagcgggc gggcgcgctc gtcgccgagc tggatcaggc
     2761 gcggacccgg ctctcccgcc tgaccctggc ggtgtacggg tcgggaggcg ggcagaccga
     2821 ccgctccgcg ctcgaccgcg cccggctcga gcgcgaggag gcggagcggg cgctcgcacg
     2881 gtggagcgcg agcgcggggc tgccgcgggc gcggcgcgac gtcggcctcg cggaggtgcg
     2941 cgccgcgctg ccggagcgct cggccctgat cgggttcgta cgctacgcgc gcgccctgcc
     3001 gccttccgcc gagggatcct cggcgccgga gccgccgcgc cccgagtacc tggcgttcgt
     3061 cctgcgtccg gagacggacg acatcgggct ggtcgcgctc ggcccggcgg atcggatcga
     3121 ggcgctggtg cgggactgga ggcgcgaggc gggccacccg ccggacggac gcgaggcgat
     3181 gggccgctac cgcgccgccg cgcgaggcct ggcgcaggcg gtctggaccc cgctccagcg
     3241 cccgctcgcg gaccgggacc tcgtgctgat cgttccggat ggagcgctgc agttcgtcag
     3301 ctggccgtcg cttccgtcct ccgccggcgc ctatctcgtc gagagcggtc cgacgattca
     3361 tctgctctcc gccgagcgcg gcgtggcgct cgacgggcag gcgccagccg ggcgggggct
     3421 gctcgcgatc ggcggacccg atttcggcgc cgcgccggcg tcatcgccgt gcgccgggtc
     3481 gtcgatcggc gcgctgaccg cgctgccgag cgccgccgag gaggcgcgcg acgtcggcgc
     3541 gctgtggaag cgggaggagg gggcggggtc gcgtgagacc ggagacgtct cgatcttcac
     3601 cgggcgcggg gcgagcgagg cggcgttccg atcgtcggcg ccggggcgcc gggtgctgca
     3661 cctcgccacg cacgggttct acctggcgga ccgctgcgat ccggacagcc ggggcggggc
     3721 ggggcgcgcc gcgacggtga gcgatgccgc cgaggccgga tcgatcgaca cggcgctcgc
     3781 acggaccggt ctggcgttcg ccggcgccaa ccgcgcctgg ggagatcgcg gcgccgacgg
     3841 cggcgccgac gggctcctga ccgcgcagga gatcgccgcc ctcgacctgc gcggcgtgga
     3901 atgggcggtc ctgtcggcct gcggcagcag cctcggacag gccgaggcgg gggaggggat
     3961 cctggggctg cggcgcacct tcgaaatcgc cggagcccgc tccctcgtca cgagcctctg
     4021 ggatctcgag gaccactcgg cgcggcggtg gatccgggag ctctatcggg cccgcctcca
     4081 cggcgccagc accgccggag cggtgcggca ggcgagccgg tcgatcctgg agtcccggcg
     4141 ccgggccggg ctcgcggacc atccttatta ctgggctccc ttcgttgcgg ccggcgactg
     4201 gcgctgagtc ccctcaggga cgcgcggagc cattcgcttc cggctggaag cggaaggggt
     4261 agcggtactc gatgcgcgtc cccgcacccg tctcaccctc cgcctgctcc acctgtgaca
     4321 ggacgagcgt ataggagccg gcgtggcgga acggcgctgc tgaaaagacc gcggtgctgc
     4381 gtcccatctc gtcgtggcct cccaccggga ccgcgtcggc gatcggccgt ccctcctcgt
     4441 cttccatccg gacggtcgcc gcgcggccga cgccgggaat gacgaagctc acctcgatcc
     4501 gctctgcccc ctcggggagg cggaggaccg gcgtctcctc ggcgccgcgg accggcccgg
     4561 ggccgagatc caggcgggcg agcatcgcgg gagccgcggg cggcggcccc gcgggcgcgg
     4621 cagggccggg cgcgggggcc gcatctccgg aggcgctgcg cggatccggt cgctccgagc
     4681 ggagaagcgc cgcgaggagg acccccgcga gcatcgccgc gatccccgcg gcgagggcgg
     4741 tccgccggcg gcgaccgggc tgcggcttgg gctgcgcggg gaacgggatc gccgccggcc
     4801 gcgcctccgg cgcggcggca ttcgaggggg cgccgtggag gcgcacgagc tcggcgatct
     4861 ccccgaggtc gtggaggtcg cggacgcaat cggggcagga ggcgaggtgt gcctggacct
     4921 cgcccgcctc gctcccctcc aggctgccgt tcaggtacca gggcaggagc tcccgcacgg
     4981 cggaccggca ggcggatttc acggccgttc tcccgcctct gcgagcagcg cgcggcggcg
     5041 ctcgaggagg cgatcccgga ggcgcgccat gcgcgacttg acggtccctt ccgggatgcc
     5101 aagcagggcg gccacctcgg cgtacgactt gtagtcgcgg aacaccagcg taatcatcag
     5161 acggtcctcc tccgtcagtc cgtccatgag gcgcgccagc atctgacgcg cggcccagga
     5221 gcgcgaagcg ggcgattcct tttcatccac cggcagtt
//
LOCUS       DASRIQ010000573         8716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_997465, whole genome shotgun sequence.
ACCESSION   DASRIQ010000573 DASRIQ010000000
VERSION     DASRIQ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997465"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFD06_14175"
     CDS             complement(<1..760)
                     /locus_tag="VFD06_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN04727.1"
                     /translation="MASWLLRNGRVVDPSQGLDGPHDLLIVNGRIAEVRPVGSRVDSA
                     PARGAGSGKVPAGTQVIDLSGAVVVPGLVDMHVHLREPGQEHKEVIETGTRAAAAGGF
                     TSVACMPNTEPVNDSLSVTEYIVQEARRRGAVNVYPIGAVSKGSAGEELAEIGDMVRA
                     GAVAVSDDGRPVSSSNLMRMALEYTRLFDIPVINHCEDRSLSAAGVMNEGAVATALGL
                     KGNPRASESVAVARDLALAEMTGGRLHLAHLSTRA"
     gene            complement(741..1811)
                     /locus_tag="VFD06_14180"
     CDS             complement(741..1811)
                     /locus_tag="VFD06_14180"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZN04728.1"
                     /translation="MDIVEEIGATAARPRRRPGSAGRIAPAAAAAVGTARPETPPGPR
                     TALRDRHLLGIQGLEPDEITLVLDTARVMAEIATRPVRKIPTLRAKTVVNFFMEPSTR
                     TRSSFEIAEKRLSADTLNFSPSASSVTKGESLLDTARNLEAMSPDMIVIRHKASGAPH
                     FLARHCRSAIINAGDGWHEHPTQALLDAYTIRERKGRLKGLTVAIVGDLMHSRVLRSN
                     LFLLRTMGAKVRLCGPPTLVPAGLESLGAEVHLRLEPALEGADVIMMLRVQRERQKGS
                     AFPTAREYFNLFGMTRERVRLAKKDAIIMHPGPMNRGVEIDSAVADGPASVILEQVTN
                     GVAVRMAVLYLLAGGSLGELAS"
     gene            complement(1921..2469)
                     /gene="pyrR"
                     /locus_tag="VFD06_14185"
                     /pseudo
     CDS             complement(1921..2469)
                     /gene="pyrR"
                     /locus_tag="VFD06_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
     gene            complement(2639..4294)
                     /locus_tag="VFD06_14190"
     CDS             complement(2639..4294)
                     /locus_tag="VFD06_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN04729.1"
                     /translation="MSTPARLVLHAAIAGLYAALALVVFLRLLHPEPPRLRFIVAMLP
                     VAAAYTAVAAILLPLLYGLVRFFASHRLHVPSFSPRYAMTFLVVALTPILASIGVTLS
                     QSRRAIDEAAAGRVKLLLAAVGGAWIYAGVVCLVPRLKRSLALQASAAGVALAALLAP
                     GVLPAGMPGPDPARPPEGLPPVARRLVLLEIDGADLEDLLGLQTRGLLPSLSRLRREG
                     TYGRLESLLPCEAAVVRTTLVTGQLPWRSGVRGPFERRLLGQPVGLSIVPAGLGFDRL
                     LSPVMTRRRLTVEDRRGPALWDMVRSAGGTARAAGWEVDLDHPGGPEIPGLSEVRTLA
                     AEFGEGETSYFNMPLATSVDLARALAADQAAGEALDPPLEGHGLVAVSLPGLDRVAHR
                     FLRFARPDDFGNVRPAEVDEYGRVLERYYVRIDALVERARALAGPDGWLFVTSAHGIE
                     PAPLRQRLLPGRSGGREALSGAHADGPSGFLFAVGPGVRAGQRFGRAALADVAPTALY
                     LLGLPIARDLDGRILDSILDPEEVRARPVQVIDSYGPRPASPG"
     gene            4359..5195
                     /locus_tag="VFD06_14195"
     CDS             4359..5195
                     /locus_tag="VFD06_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HZN04730.1"
                     /translation="MPFDTAARILSHDALPSDHFLLTLDAPRLARAARPGQFVMLQAQ
                     PGTDPLLRRPMSVCRVGGTPRRRVEILYKVTGHGTEMLSRQPKGAVLAMLGPLGNGFR
                     LPPRASGRRGSGRAPAQEAPRQVLVAGGVGIAIFPFLAEALGKLGHKPLLLFGARSAA
                     DLVALDLFGRGRVEMRFATENGSRGTRGYVTSLLAPLLEAAGPKPSLYVCGPTPMLRA
                     VGEQALAGGAPCQLALESQMPCGIGVCLGCVVAAADPNAVPPYPRVCTEGPVFEAGSV
                     RP"
     gene            5252..6187
                     /locus_tag="VFD06_14200"
     CDS             5252..6187
                     /locus_tag="VFD06_14200"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZN04731.1"
                     /translation="MRVTLGPLTLRNPVLAASGTFGYGTELQPYFDLEAIGGFVSKGL
                     SLKPRRGNAPPRTCETAAGMLNAIGLANVGVEAFLRDKLPALRGKDVVVIANVFGETK
                     DEYVAVASRLNGAPGLHAIELNLSCPNTEKGGLAFGVSPALIREVVSAVRRAAPDLPL
                     LVKLTPNITDIGEAARAAADAGADILSLVNTFLGMAIDLERRRPILDNVVGGLSGPAI
                     KPLALYMVHKVATAVRLPIVGMGGIATAEDALEFMVAGSAAVQVGTANFYDPSAPARV
                     AEGMAAWCRARGVLAVRELTGTLRLPAKEQETNAG"
     gene            complement(6221..6736)
                     /locus_tag="VFD06_14205"
     CDS             complement(6221..6736)
                     /locus_tag="VFD06_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN04732.1"
                     /translation="MSERRRALGALAFAIVVAAAPLAGCRKGEAETFGGGDVKVIAHG
                     EQVDLAAHLAAGKHTVFDFYAPWCPPCRVLGPALERLAAAQPDRLAIRKVDIVDWTTP
                     VVDQHGIEALPHLILYDASGRVAAEGDQVYTELERLFGDAAKEVIQAGTGDDTPKPAE
                     GTKAADGTQVL"
     gene            6812..7528
                     /gene="pyrF"
                     /locus_tag="VFD06_14210"
     CDS             6812..7528
                     /gene="pyrF"
                     /locus_tag="VFD06_14210"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZN04733.1"
                     /translation="MHSADRLIVALDVPTREAALSLVAAIRPKARRFKVGLELFSACG
                     PGLVREILQGGGQVFLDLKLHDIPNTAARAAVEAARLGVGMFTLHLSGGLMMARRVVD
                     EVEAHCQIHRDPRPQILGVTVLTSLAQEDLDALGITRSVDDQVAHLAGMARQAGLDGI
                     VASPREVRRLREIVGAQMLIVTPGIRPAGADHNDQVRTLTPREAIEAGSDYIVVGRPI
                     TAARDPLEAVENLLDEMEGR"
     gene            7525..8409
                     /locus_tag="VFD06_14215"
     CDS             7525..8409
                     /locus_tag="VFD06_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04734.1"
                     /translation="MRRAATACAVAMLAIATLAETAGAPAPAPEPPPLTNEDIVRLSA
                     YGTSEEVILKTIRERRADFDLDPGVVAELERVGVKPRVIEAMRRRQLESPARPGAPPP
                     APPPLAATPPPGRVDLAFDETPVKKDPPQIYALTSLPKGAPRPEDSEIGTVSDLALAI
                     LCTTGDHVPDHWDRRTPLEGAPRHELLLFRVGTTRRREHGFDVLALDRAPVAAMPLPP
                     GPHALLVVLAGRQTGSGAWRPLAADPIRVEAEPGKEIDLVLDARTELNGSRMTGFGLK
                     QVWKVRLATADAAAEPPA"
     gene            8406..>8716
                     /locus_tag="VFD06_14220"
     CDS             8406..>8716
                     /locus_tag="VFD06_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN04735.1"
                     /translation="MIAPVAAPRSLVLCGGGITGAMYEFGALHALDHACGGKFLSTQY
                     DVYVGTSGGAVVAALMANGVSPAEVGRAILHNSDDPLNFRQEDIVRIDWREIRHSFVK
                     G"
BASE COUNT         1194 a         3242 c         3103 g         1177 t
ORIGIN      
        1 tggcgcgcgt gctcaggtgc gccaggtgca gccggccacc ggtcatttcc gcgagggcca
       61 ggtcgcgcgc caccgcgacg ctctccgagg cgcgcgggtt ccccttcagc ccgagcgcgg
      121 tcgcgaccgc cccctcgttc atcacccccg cggccgacag gctccggtcc tcgcagtggt
      181 tgatcaccgg gatgtcgaag agccgcgtgt actcgagcgc catgcgcatc aggttcgagg
      241 acgacaccgg gcggccgtcg tccgagaccg cgaccgcccc ggcgcgcacc atgtcgccga
      301 tctccgccag ctcctccccc gccgagccct tgctgacggc gccgatcggg tacacgttga
      361 cggcgccccg ccggcgcgcc tcctgaacga tgtactcggt cacggagagg ctgtcgttga
      421 cgggctcggt gttcggcatg caggcgaccg aggtgaagcc ccccgccgcc gcggcgcgcg
      481 tgccggtttc gatgacctct ttgtgctcct gccccggctc gcgcaggtgc acgtgcatgt
      541 ccacgagccc cggcacgacg acggccccgg acagatcgat cacctgcgtc ccggcgggca
      601 ccttgccgct cccggcgccg cgcgccggcg ccgagtcgac gcgtgacccg accggacgca
      661 cctcggcgat cctgccgttg acgatcagga gatcgtgcgg gccgtcgagc ccctgcgacg
      721 ggtcgacgac ccggccgttt ctaagaagcc agctcgccaa gactcccccc cgccagcagg
      781 tacaggaccg ccatgcgcac cgcgacgccg ttcgtcacct gctcgaggat gaccgacgcc
      841 gggccgtcgg cgacggcgct gtcgatctcg acgccgcggt tcatcgggcc ggggtgcatg
      901 atgatggcgt ccttcttcgc cagacgcacc cgctcacgcg tcatgccgaa gaggttgaag
      961 tactcgcgcg ccgtcgggaa ggcgctcccc ttctggcgct cccgctggac gcgcagcatc
     1021 atgatgacgt ccgccccttc gagcgccggc tcgaggcgca ggtgcacctc ggccccgagc
     1081 gactcgagcc cggctggcac cagcgtcggc gggccgcaca gccgcacctt cgcccccatc
     1141 gtccgcaaca ggaagagatt gctgcggagc acgcggctgt gcatcaggtc gcccacgatc
     1201 gcgaccgtca gccccttcag gcgccccttg cgctcgcgga tggtgtaagc atcgaggagc
     1261 gcctgcgtcg ggtgctcgtg ccacccgtcg ccggcgttga tgatcgccga gcggcagtgc
     1321 cgcgccagga agtggggcgc cccgctcgcc ttgtggcgga tgacgatcat gtccggcgac
     1381 atcgcctcga ggttccgggc cgtgtcgagg agcgactccc ctttcgtgac gctcgaggcg
     1441 gacgggctga agttcagcgt gtcggccgac agccgcttct cggcgatctc gaaggaggag
     1501 cgggtgcggg tggacggctc catgaagaag ttgaccaccg tcttggcgcg cagggtcggg
     1561 atcttgcgga ccggacgcgt ggcgatctcc gccatgaccc gcgccgtgtc gagcaccagg
     1621 gtgatctcgt ccggctcgag cccctggatg ccgaggaggt gccgatccct gagagcggtg
     1681 cgcggccccg gcggcgtctc gggacgcgcc gtcccgaccg cggccgccgc ggctggcgcg
     1741 atgcgcccgg cggagccggg ccgacggcgc ggacgcgccg cggtcgctcc gatctcctcg
     1801 acgatgtcca tcagatccgc cctgccatca gattcgcttt cgcgtggccg atcgctgcgt
     1861 ccgccgcaca ggcgccacgc cgcgcgccgg gacggccacg tgcggcctca ccggcgcccg
     1921 cgcccccgcg cgcgcctcga tccgcactgc gtcctccccg tcgatctcct tcaaccgcac
     1981 cttgacctcg tcctcctcgc gcgtcggaat ctccttgccg acgtaatcgg cgtggatcgg
     2041 cagctcgcgg tggccgcggt cgatgagcac cgccagctcg atgctccgcg ggcgcccgaa
     2101 gtcgatgagc ccgtcgagcg ccgcccggat ggtccggccg gtgtacagca cgtcatccac
     2161 caggacgatc cgcttgccgt cgagcgggaa cgggatgcgg gtctcgcgca ggagcgcctg
     2221 cgctcccatg gcggtcacgt cgtcccggta cagcgtgatg tcgagcgtcc cgagcggcac
     2281 cttgcgcccc tgcggcccgg cgatgatggc ccgcagcctc tcggcgagag gcacgccgcg
     2341 ggtgcggatg ccgatcaggg ccagcgcctc gagatcggga tggtgttcga ggatctcgtg
     2401 cgccagccgg gcgaggaccc gggagatggc ggcggcgtcc atgatggccg cgcggtccgc
     2461 tcccttcatg cgcccccccg ccgcgggcgc ccgggggcga aagaaggccg cgcgggcaaa
     2521 aaaaatcccg cgtcgccgcg ggtgtcgggg ctgctcggac tgtcgcctct catcgtcctc
     2581 gtgtctcgat gaagcccgcg cagcatagcg gaccgggtga gacgctgtca agaaacgctc
     2641 atcccgggga ggcgggtcgc ggaccatagg aatcgatgac ctgcaccggc cgggcccgga
     2701 cctcctccgg gtcgaggatc gaatcgagga tgcgcccgtc gagatcccgg gcgatcggca
     2761 gcccgaggag atagagggcg gtcggggcca cgtcggcgag cgcagcccgg ccgaagcgct
     2821 gacccgcccg cacgccgggg ccgaccgcga acaggaaccc gctcggcccg tccgcgtgcg
     2881 caccggagag ggcctcgcgt ccgccggagc ggcccggcag gagccgctgc cgcagcggcg
     2941 ccggctcgat gccgtgggcc gaggtgacga acagccagcc gtccgggccg gcgagtgcgc
     3001 gggcccgctc cacgagcgcg tcgatgcgca cgtagtagcg ctccagcacc cggccgtact
     3061 cgtccacctc cgccggccgg acgttgccga agtcgtccgg ccgcgcgaaa cgcaggaacc
     3121 ggtgcgccac ccggtccagc cccggaagcg acacggcgac cagaccatgt ccttcgaggg
     3181 gggggtcgag cgcctccccc gcggcttggt cggcggccag ggcccgggcg aggtcgaccg
     3241 acgtcgccag cggcatattg aaataggacg tctctccttc gccgaattcc gccgccagcg
     3301 tcctcacctc ggagaggccg gggatctccg gtccgcccgg gtggtcgaga tcgacctccc
     3361 accccgccgc gcgcgccgtc cctccggcgc ttcgcaccat gtcccacaac gccgggccgc
     3421 ggcgatcctc caccgtcagt cgcctgcgcg tcatgacagg cgagagcagc cggtcgaagc
     3481 cgagcccggc ggggacgatg ctgaggccga ccggctggcc gagcagtcgc cgctcgaagg
     3541 gaccgcgcac gccgctcctc cacggcagct gcccggtcac gagcgtggtg cggaccacgg
     3601 ccgcttcgca cggcaggagc gattcgagcc ggccgtaggt cccctcccgc cgcaggcgcg
     3661 acagcgacgg caggagaccc cgggtctgca ggccgaggag gtcttcgaga tccgccccgt
     3721 cgatctcgag gaggacgagg cgacgcgcca ccggcggcag cccctccggg ggcctggccg
     3781 ggtccggacc cggcatcccg gccggaagca ccccgggcgc caggagcgcc gccagcgcca
     3841 cgccggcggc ggaggcctgc agggccaggc tccgcttgag gcgcggtacg agacagacga
     3901 ccccggcgta gatccacgcg ccgccgaccg cggccaggag cagcttcacc cgtccggccg
     3961 ccgcctcgtc gatggcgcgg cgggactgcg acagggtcac gccgatcgac gccaggatcg
     4021 gcgtcagcgc gaccacgagg aacgtcatgg cgtagcgcgg gctgaaggac ggcacgtgca
     4081 ggcggtgcga ggcgaagaag cgcacaagcc cgtagagcag cggcagcagg atcgccgcga
     4141 cggccgtgta ggccgccgcc accggcagca tggcgacgat gaagcggagc cgcggcggct
     4201 ccgggtgcag caggcgcagg aagacgacga gggcgagggc ggcgtacagc cccgcgatcg
     4261 cggcatgcag gacgagacgc gccggtgttg acacgcgcgc gtgatggtag ggggccgtcc
     4321 cccaaagatc aaggaggtgt gctacagtcc cgcgttcgat gccgttcgac accgccgcgc
     4381 ggatcctgtc gcacgacgcg ctgccttccg atcacttcct cctgaccctc gacgcgccgc
     4441 gcctggcgcg cgccgcccgt cccggccagt tcgtcatgct gcaggcgcag ccggggaccg
     4501 acccgctgct gcgccggccg atgagtgtct gccgcgtcgg cggaacgccg cggcgccggg
     4561 tggagatcct ctacaaggtg accgggcacg gcaccgagat gctgtcgcgg cagccgaagg
     4621 gggccgtcct cgcgatgctc gggccgctcg ggaacggctt ccgcctgccg ccgcgagcgt
     4681 cggggcgccg cgggtccggt cgcgcgccgg cgcaggaagc gccgcgccag gtcctggtcg
     4741 cgggtggagt cgggatcgcc atcttcccgt tcctcgccga ggcgctcggg aagctcggtc
     4801 acaagccgct gctcctcttc ggcgcccgct cggcggccga cctcgtggcg ctcgacctgt
     4861 tcggacgggg ccgcgtcgag atgcgtttcg cgaccgagaa cggcagccgc ggaacgcgcg
     4921 gttacgtgac ctcgctcctc gcgccgctcc tcgaggcggc cggcccgaag ccgtcgctct
     4981 acgtctgcgg cccgacgccg atgctccgcg ccgtcggcga gcaggcgctg gcgggcggcg
     5041 ccccctgcca gctggccctc gaatcgcaga tgccgtgcgg catcggcgtc tgcctcggct
     5101 gcgtggtggc ggccgccgac ccgaacgccg tcccgccgta tccccgcgtc tgcaccgaag
     5161 ggccggtgtt cgaggccggg agcgtccgcc cgtgaagcgg cccgtcccct cccccccttc
     5221 gaagccgaag gcgccatcgg gcccagtgga catgcgcgtg accctcggcc ccctgacgct
     5281 caggaatccg gtcctcgccg cgagcggaac cttcggctac ggcaccgagc tgcagccgta
     5341 tttcgatctc gaggcgatcg gcggcttcgt cagcaagggg ctgtcgctca agccgcgccg
     5401 cggcaacgct ccaccgcgca cctgcgagac ggcggccggc atgctcaacg ccatcggcct
     5461 cgccaacgtc ggcgtcgagg cgtttctgcg ggacaagctg ccggcgctcc gcggcaagga
     5521 cgtcgtcgtc atcgccaacg tgttcgggga gacgaaggac gagtacgtcg cggtcgcgtc
     5581 acgcctcaac ggcgcgccgg ggctgcacgc catcgagctg aacctctcgt gccccaacac
     5641 cgagaaaggg ggcctggcgt tcggggtctc gccggcgctc atccgcgagg tggtgtcggc
     5701 ggtgcggcgc gccgcccccg atctgccgct tctcgtgaag ctgacaccga acatcaccga
     5761 catcggcgag gcggcccgcg ccgcggccga cgccggcgcc gacatcctgt cgctcgtcaa
     5821 caccttcctc ggcatggcga tcgatctcga gcggcgccga ccgatcctcg acaatgtcgt
     5881 cggcgggctc tcgggcccgg cgatcaagcc gctcgccctt tacatggttc acaaggtggc
     5941 gacggcggtg cgcctgccga tcgtcggcat gggcgggatc gcgaccgcgg aggacgccct
     6001 cgagttcatg gtggcgggaa gcgccgcggt gcaggtgggg acggccaact tctacgaccc
     6061 cagcgccccc gcccgcgtgg ccgaggggat ggcggcgtgg tgccgggcgc gcggcgtgct
     6121 cgcggtgcgc gagctcaccg gaacgctgcg tctcccggcg aaggaacagg agacgaacgc
     6181 gggataggga atccgggcct gcgggacggc gacgatctcg ctagaggacc tgggtgccgt
     6241 cggccgcctt ggtcccttcg gccggcttgg gggtgtcgtc gccggttccg gcctggatga
     6301 cctctttcgc tgcgtccccg aacagccgct ccagctccgt gtagacctgg tctccctcag
     6361 cggccacacg gccggacgcg tcatagagga tgaggtgcgg cagcgcttcg atgccgtgct
     6421 ggtccacgac cggcgtggtc cagtcgacga tgtccacctt ccggatggcg aggcgatccg
     6481 gttgcgcggc ggccaggcgc tcgagcgccg gccccagcac gcggcacggc ggacaccacg
     6541 gggcgtagaa gtcgaacacc gtgtgcttgc cggcggccag gtgcgccgcc aggtcgacct
     6601 gctcgccgtg ggcgatcacc ttcacgtcgc cgccgccgaa ggtctcggcc tcgcccttcc
     6661 ggcagccggc gagcggcgcc gccgcgacca cgatcgcgaa cgccagcgcg ccgagggccc
     6721 ttcgtcgttc cgacatgcgc tcgattatcc gcacgccgcg cgcccgtggc aaccttgcac
     6781 gaccgttccg cttctgtgac aattcttcgc catgcactcc gccgaccgtc tcatcgtggc
     6841 gctcgacgtc ccgacgcgcg aggcggcgct gtcgctggtc gcggcgattc gcccgaaagc
     6901 ccggcgcttc aaggtcggcc tcgagctgtt ctccgcctgc ggtccggggc tcgtccgcga
     6961 gatcctgcag ggcggcggac aggtcttcct cgatctgaag ctgcacgaca tcccgaacac
     7021 cgcggcgcgc gccgcggtcg aggcggcgcg gctgggcgtg ggcatgttca cgctgcacct
     7081 ctccggcggg ctgatgatgg cgcgccgcgt cgtggacgag gtcgaggcgc actgccagat
     7141 ccaccgggat ccgcgcccgc agatcctcgg cgtgaccgtg ctgacgagcc tggcgcagga
     7201 ggatctcgac gccctcggca tcacccggtc ggtggacgac caggtggcgc acctcgccgg
     7261 gatggcccgg caggcggggc tcgacggcat cgtcgcctcg ccgcgcgaag tgcggcgcct
     7321 gcgggagatc gtcggggcgc agatgctgat cgtcaccccc gggatccgtc ccgccggcgc
     7381 ggatcacaac gaccaggtgc gcaccctgac gccgcgcgag gcgatcgagg ccggctccga
     7441 ctacatcgtg gtcggccggc cgatcaccgc cgcccgcgat cccctggagg cggtggagaa
     7501 cctcctcgac gagatggaag gtcgatgagg cgggcggcca ccgcctgcgc ggtcgccatg
     7561 ctcgcgatcg ccacgctcgc cgagacggcc ggcgcgccgg ccccggcccc cgagccgccg
     7621 cctctgacca acgaagacat cgtccggctc agcgcgtacg gcacctccga agaggtcatt
     7681 ctcaagacga tccgcgagcg ccgcgccgac ttcgatctcg atccgggtgt cgtcgcggag
     7741 ctggagcggg tcggcgtcaa gccgcgcgtc atcgaggcga tgcggcgccg gcagctcgaa
     7801 tcgccggccc gtccgggcgc tcccccgccg gccccgccgc cgctcgcggc caccccgccc
     7861 ccggggcgcg tcgatctcgc cttcgacgag accccggtga agaaggaccc gccgcagatc
     7921 tacgcgctca ccagcctgcc gaaaggggcg ccgcgccccg aggactcgga gatcggcacg
     7981 gtgagcgatc tcgcgctcgc catcctgtgc acgaccggcg atcatgtccc ggatcactgg
     8041 gaccggcgca cgccgctcga aggggcgccg cgccacgagc tcctgctctt ccgggtcgga
     8101 acgacccggc gccgcgagca cggcttcgac gtgctggcgc tcgaccgcgc gccggtggcg
     8161 gcgatgccgc tgccgcccgg cccgcacgcg ctcctcgtcg tgctggcggg acggcagacc
     8221 gggtcgggag cctggcggcc gctcgccgcc gacccgatcc gtgtcgaggc ggagccgggg
     8281 aaggagatcg atctcgtcct cgacgcgcgg acggagctga acggaagccg catgaccggg
     8341 ttcggcctga agcaggtctg gaaggtgcgc ctggccaccg ccgacgccgc ggcggagccg
     8401 ccggcgtgat cgccccggtc gcggctcctc gcagcctcgt cctgtgcggc ggagggatca
     8461 ccggcgccat gtacgagttc ggcgccctgc acgccctcga ccacgcgtgc ggcgggaagt
     8521 tcctctcgac ccagtacgac gtctacgtcg gcacgagcgg cggcgccgtg gtcgcggccc
     8581 tcatggcgaa cggcgtctcc cccgcggagg tcggccgggc catccttcac aacagcgacg
     8641 acccgctgaa cttccggcag gaggacatcg tccggatcga ctggcgcgag atccgccatt
     8701 cgttcgtgaa ggggat
//
LOCUS       DASRIQ010000574         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_761483, whole genome shotgun sequence.
ACCESSION   DASRIQ010000574 DASRIQ010000000
VERSION     DASRIQ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761483"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1463)
                     /locus_tag="VFD06_14225"
     CDS             complement(54..1463)
                     /locus_tag="VFD06_14225"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN04736.1"
                     /translation="MKGARIAPIATLATTLALPLLLLTPGCLGQRRPNILLVVADTAR
                     ADRFLQEGERAAVAPRVAALAQGGAAYLDARSPSPWTLPAHASLFTGLYPSSHGAEAG
                     HLKLREDAPFLAERLRRAGYRTQAYIANPWVAKDYNFHVGFDTFDEVWRAVKGTEGEM
                     GAEAIVGKIARWLDWRAGNDDAKAKPFFVFVNFFEPHLPYNPPEPERSRLLPPGADSE
                     RIERLRRFKHPDEVRAILGQTRLDAADRRVLAALYDGEVAYADRRIGDLVDLLEARGL
                     LDDTVVAVTSDHGEMLGEHGLYDHKLSLYEPVLRIPLVLRYPARVEAGQRIASSVMLQ
                     DLYPTLLALAGVEPGPSQEARRLPGIRGLKAGPIRGTSLDDPIVAEYARPAEFLPVMR
                     ELAPGADLSHWDAALVAFTYGTRKLVWSSAGPAELYDLAADPGESEPIAASGPQEEIA
                     SNRAADRAARLAQPPAAAP"
     gene            complement(1460..>2061)
                     /locus_tag="VFD06_14230"
     CDS             complement(1460..>2061)
                     /locus_tag="VFD06_14230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZN04737.1"
                     /translation="PIDRTSWRAQLALTVPIGNRSAQADYARAEADLAQAQSLMAQAE
                     QTARFDVRQAVRQVETTLQRVKAGRVNVRLQAEKLRAEQKKFENGMSTSFQVLTFQDD
                     LFTAKTRENLATADYNKALVELSRSKGTLLADKGILVQPPGGESEKNGTPQASAALHR
                     FWRSPQDAWLDPGLRLGDAAIDPIHLPADFTWTKPTRGR"
BASE COUNT          271 a          744 c          755 g          291 t
ORIGIN      
        1 ctcgcggcgg cgcgccagcg ccggggacgg ggacggggca gcgcttccgg gcgctacggc
       61 gcggcggccg gcggctgggc caggcgcgcg gcacggtcgg cggcgcggtt cgacgcgatc
      121 tcctcctggg ggccggacgc cgcaatcggc tccgactccc cgggatcggc ggcgaggtcg
      181 tagagctccg ccggcccggc ggacgaccac acgagcttgc gcgtcccgta ggtgaacgcc
      241 acgagcgcgg cgtcccagtg cgacaggtcg gcccccggcg ccagctcccg catcaccggc
      301 aggaactccg ccggtcgcgc gtactcggcg acgatcggat cgtcgaggga cgtcccccgg
      361 atcggcccgg ccttgagtcc ccggatcccc ggaagccgcc gcgcctcctg cgacggaccc
      421 ggctcgaccc cggcgagggc gaggagcgtc gggtagagat cctggagcat cacggaggag
      481 gcgatccgct gccccgcctc caccctggcc ggatagcgca ggacgagcgg gatccggagg
      541 accggctcgt acaggctcag cttgtgatcg tagaggccgt gctctccgag catctcgccg
      601 tgatcggagg tgaccgccac gaccgtgtcg tcgaggagcc cgcgcgcctc gagcagatcg
      661 acgagatccc cgatgcgccg gtcggcatag gcgacctcgc cgtcgtacag ggcggcgagc
      721 accctccggt ccgccgcgtc gagccgcgtc tgtccgagga tcgcccggac ctcgtccgga
      781 tgcttgaacc gtcgcaggcg ctcgatccgc tcggagtcgg cccccggcgg caggaggcgc
      841 gagcgctccg gctccggcgg gttgtacggc aggtgcggct cgaagaagtt gacgaagacg
      901 aagaacggct tcgccttcgc gtcgtcgttg ccggcgcgcc agtcgagcca gcgggcgatc
      961 ttcccgacga tcgcctccgc ccccatctcc ccctcggtcc ccttcaccgc gcgccagacc
     1021 tcgtcgaagg tgtcgaagcc gacgtggaag ttgtagtcct tggccaccca aggattcgcg
     1081 atgtaggcct gggtgcggta gccggcgcgc cgcaggcgtt cggcgaggaa cggcgcgtcc
     1141 tcccgaagct tgaggtgccc cgcctccgcg ccgtgcgacg acgggtagag accggtgaac
     1201 aggctggcgt gcgcgggcag cgtccagggg gacggggatc gcgcgtcgag gtacgccgcc
     1261 ccgccttgcg ccagcgccgc gacgcgcggc gcgaccgccg cacgctcccc ctcctgcagg
     1321 aagcggtcgg cgcgcgccgt gtcggcgacg accagcagga tgttcggacg gcgctggccg
     1381 agacagccgg gcgtcagaag caggagcggc agcgcgagcg tcgtggcgag cgtcgcgatc
     1441 ggcgcgatgc gggcgccctt caacgcccgc gcgtcggctt cgtccaggtg aagtcggccg
     1501 gcaggtgaat cggatcgatc gcggcatcgc ccaggcgcag cccggggtcg agccacgcgt
     1561 cctgcgggct ccgccagaag cggtggagcg cggccgaggc ctgcggcgtc ccgttcttct
     1621 cgctttcgcc gcccggtggc tgcaccagga tccccttgtc ggcgagcagc gtccccttgg
     1681 agcgcgacag ctcgacgagc gccttgttgt aatccgccgt cgccaggttc tcgcgcgtct
     1741 tggcggtgaa caggtcgtcc tggaacgtca gcacctggaa cgaggtgctc atgccgttct
     1801 cgaacttctt ctgctcggct cgcagcttct ccgcctgcag ccggacgttg acccggcccg
     1861 ccttgacccg ctgcagggtc gtctcgacct ggcgcaccgc ctggcggacg tcgaaccggg
     1921 ccgtctgctc cgcctgcgcc atcagcgact gcgcctgggc gaggtcggcc tcggcccggg
     1981 cgtagtcggc ctgcgccgat cgattgccga tgggtaccgt cagcgccagc tgggcgcgcc
     2041 acgaggtgcg gtcgatcgga t
//
LOCUS       DASRIQ010000575         6405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3101806, whole genome shotgun sequence.
ACCESSION   DASRIQ010000575 DASRIQ010000000
VERSION     DASRIQ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3101806"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VFD06_14235"
     CDS             complement(<1..1104)
                     /locus_tag="VFD06_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04738.1"
                     /translation="MNRSLRLLAVAALVFLPPRDVDAAGEAARDGRREIGALVMEGVP
                     DLPPDLLERMRPYQNTRSAGVHDWDPQGRGLLVGTRFGETSQVHRVDRPGGDRRQITF
                     YPEPIASATTCPAVATNGFLFSKDVGGAENFQIYFRDLGRGTTTLVTDGRSRNTGAVW
                     SNKGDRFAYASNRRNGRDMDLYVADLASPAGARRVLEREGAWSVLDWSPDDAALLVQR
                     NVSVNESYLHILRLVPGQAGPTDAALEPVNPKPTPIAYGAARFARRGNGVYLISDEGS
                     EYRRLRYYDLATKGQAVLSGDIEWDVEALDVSRAGDRLAYLINEGGSSRLMLLDTASR
                     KPTRPSGIPDGVMENPHFSPDGTRLAFTLGAATV"
     gene            1181..3208
                     /locus_tag="VFD06_14240"
     CDS             1181..3208
                     /locus_tag="VFD06_14240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN04739.1"
                     /translation="MSLRPRSAASLPVVAVLLVLSACTAVREPALESRQVPEYPIEDF
                     LGTTAYMGASFTSDGAAILASNDSTGVMNVYAVPAAGGDPKPLTRSTTESVLVAAAFP
                     ADGRFLYLSDQGGNELSHLFIQEIDGSTRDLTPGEKVKATFVGWAPDERSFFVLTNER
                     DPRHFDLYEYTADGYGRTLLYRNEEGFDFGGISPDRRLVALRRNLTDTDSDIVLHDRQ
                     SGKARVLTAHQGEVFNDPQSFSRDAKSLYYLTDEASEFRYLVRMDLESGKRDVIEKPD
                     WDVIEAGLSKNGKYLWLEVNNDARSEIRLYEAATMQPVRLPALPKAAITSVTFSRDET
                     QAAFYVNGSRQPNDLYVLDLATGRSRPLTRSLSPRLKAEDLVEAETARFRSFDNTEVP
                     GILYKPHRASASSKAPALVWVHGGPGGQSRVTYSGLIQYLVNHGYTVYAINNRGSSGY
                     GRTFYKMDDRKHGEGDLDDCVASKKFLAATGYVDPARIGIIGGSYGGYMTLAALTFRP
                     QEFAVGVDIFGVSNWVRTLQSIPPYWESFRKALYTELGDPSTDEAYLRKISPLFHASE
                     IRKPLLVLQGANDPRVLKVESDEIVAAAKNSGVPVEYLVFEDEGHGFRKKENQNKGYR
                     AIVEFLDVHLKGAKPKGPDGVTPATTAEAPAAAAPVAAPAAAPETGDAARK"
     gene            3232..4248
                     /locus_tag="VFD06_14245"
     CDS             3232..4248
                     /locus_tag="VFD06_14245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN04740.1"
                     /translation="MTIVDVAVAAIAGAVLLLLGGIALAVFQESRPDRVPALLYHRFL
                     HPGLRDQARALGVDLSYVTFADAFDEQMGFLERAGYAPITLRDFVAWQRGEAALPAKP
                     ILITCDDGFESVHRFAWPVWRARRMKATVFVTPDREAWNFRKHSALDGPLTPAQVREM
                     AAGGVAIESHGMTHAYLSRLSRDQVLWELRESKRVLEALRGEPVRFFAVPGGDYDARL
                     RGLLQEAGYEASFCVLKGTNRRGSDPYRLRRLTVGPDHRLEDFARLLHPATCWKLRLV
                     STCHEWMTRVLGPQRSERFEVALKRLGARRLFLPGAQARLWTAAILVLGLTLVLLAAW
                     VVLR"
     gene            complement(4259..5404)
                     /locus_tag="VFD06_14250"
     CDS             complement(4259..5404)
                     /locus_tag="VFD06_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN04741.1"
                     /translation="MRSRLQGLLNRGAPGKRFHRAPGSILNQAARYWRVLSPYATPRK
                     VANALLCHAEMALRRPVIRSTPYILRIEATNTCNLRCPGCATGLRINPTPKGFLAPEE
                     LRRVLDAVAPNLLITRLDGLGEPLLHPQIADLIALAHDRGSATAISTHFGEKTLAAGQ
                     ARRLVEAGLDYIIVSIDGPDQESYEKYRVGGRLETVKEHVRELVAARAAARRSNPLIE
                     VQFIGFDHNRHLADRMPALAESLGADRLLLKMVHPEAHRERRAADYEGHPCFFQYTTW
                     TIGWNGVRKLCVNAWGDTEPMQPSFRPEADLMGRDRNEPYLVALRRFLAGNRDMTRLE
                     PILGDPKSRDLSYRVPRGPGLCRCLNCATTGMVVDRNRAWLDDYICT"
     gene            complement(5505..5969)
                     /locus_tag="VFD06_14255"
     CDS             complement(5505..5969)
                     /locus_tag="VFD06_14255"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HZN04742.1"
                     /translation="MTAKPAAGLYTIVALKLGKCALLLGLAFGIYSLMDNDLRVELDR
                     FLRFVRMDPEHTFFASLGERLLTITPERIRFLASGTFLYGLLLMVESAGLMRRASWAA
                     WLAIGETAFFIPIEVFELVRRFSGTIAVILVVNAVIVWYLLRNRRRLFHHGG"
     gene            complement(6010..>6405)
                     /locus_tag="VFD06_14260"
     CDS             complement(6010..>6405)
                     /locus_tag="VFD06_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZN04743.1"
                     /translation="LVETVKGYAAHEKEVFENVAAARSRLAGATTPQEATAANQGLTS
                     ALGRLLAIAENYPQLKANENFIRLQDELAGSENRIATERMRYNEAVRGYNTLIKRFPG
                     NIVANLFSFGEKDYFEADAATRETPKVKF"
BASE COUNT          972 a         2213 c         2243 g          977 t
ORIGIN      
        1 gacggtcgcc gctccgaggg tgaaggcgag ccgggtccca tcgggggaga agtgcgggtt
       61 ttccatcact ccatccggga tcccggaggg ccgggtcggc ttccgactcg ccgtgtcgag
      121 cagcatcagc cggctgctcc ctccctcgtt gatcaggtag gcgagccggt caccggcgcg
      181 cgagacgtcg agcgcctcga cgtcccattc gatgtccccg ctcagaacgg cctggccctt
      241 cgttgcgagg tcgtagtagc gcagccggcg gtactcgctc ccctcgtcgg agatgaggta
      301 gacgccgttc cccctgcggg cgaagcgggc cgctccgtag gcgatgggag tcggtttcgg
      361 gttcaccggc tcgagcgcgg catcggtcgg gccggcctga ccggggacga gacgcaggat
      421 gtgcaggtag ctctcgttga ccgacacgtt gcgctgcacc aggagcgccg cgtcgtccgg
      481 agaccagtcg agcaccgacc aggccccttc gcgctcgagc acccggcggg cgccggcggg
      541 cgaggcgaga tcggcgacgt agagatccat gtcgcgtccg ttgcggcggt tgctggcgta
      601 cgcgaagcgg tcccccttgt tcgaccagac ggcgccggtg ttgcgcgagc ggccgtcggt
      661 cacgagggtc gtcgtcccgc gtccgaggtc gcggaagtag atctggaaat tttccgcccc
      721 tccgacgtcc ttggagaaga ggaacccgtt ggtcgccacc gccgggcagg tggtggccga
      781 ggcgatcggc tccggataga aggtgatctg ccggcggtcc ccgccgggac gatcgacgcg
      841 gtgcacctgc gaggtctcgc cgaagcgggt gccgaccagc agaccgcgcc cctgcgggtc
      901 ccagtcgtgc acccccgccg agcgggtgtt ctggtacggc cgcatccgct ccaggaggtc
      961 gggcgggagg tccggcaccc cttccatcac gagcgcgccg atctcccggc ggccatcgcg
     1021 ggccgcctct ccggccgcat cgacatcccg ggggggaaga aacacgagag cggcgaccgc
     1081 gagcaggcgc agggaacgat tcactgtggc gaccctccga ggcgcccagt ctacgttaga
     1141 atgcggccgg tccaatccgc atccgcacag gagcctcacc atgtcgcttc gtccccgatc
     1201 cgccgcgtcg ctgccggtcg tcgccgtcct gctcgtcctc tccgcctgca ccgccgtgcg
     1261 cgagccggcg ctcgagagcc gccaggtccc cgagtacccg atcgaggact tcctcggcac
     1321 caccgcctac atgggggcgt ccttcacctc cgatggcgcg gcgatcctgg cgagcaacga
     1381 ctcgaccggc gtcatgaacg tctacgccgt gccggcggcc ggcggggatc cgaagccgct
     1441 gacccgctcg acgaccgagt cggtcctggt ggcggcggcg ttccccgccg atggccgttt
     1501 tctctacctc agcgaccagg gggggaacga gctgtcgcac ctgttcatcc aggagatcga
     1561 cggcagcacg cgcgacctga cccccggaga gaaggtgaag gcgaccttcg tcggctgggc
     1621 cccggacgag cgctccttct tcgtgctcac caacgagcgc gatccgcggc atttcgacct
     1681 gtacgaatac accgccgacg gctacggccg gaccctcctg taccgcaacg aggaagggtt
     1741 cgacttcggc ggcatctcgc ccgatcggcg gctcgtcgcc ctgcgccgca atctcaccga
     1801 caccgactcc gacatcgtgc tgcacgaccg gcagagcggg aaggcccgcg tgctgacggc
     1861 gcaccagggg gaggtgttca acgatccgca gagcttcagc cgcgacgcga agtcgctcta
     1921 ctacctgacc gacgaggcct cggagttccg ctatctcgtg cgcatggatc tcgagagcgg
     1981 caagcgcgac gtgatcgaga agccggactg ggacgtcatc gaggctggcc tgtcgaagaa
     2041 cggcaagtac ctgtggctcg aggtcaacaa cgacgcccgc tcggagatcc ggctgtacga
     2101 ggcggcgacg atgcagccgg tgcggctgcc ggcgctcccg aaggccgcca tcacctcggt
     2161 caccttctcg cgcgacgaga cgcaggcagc cttctacgtc aacggcagcc ggcagccgaa
     2221 cgacctgtat gtgctcgacc tcgccaccgg gcgcagccgc ccgctgacgc gcagcctgag
     2281 cccgcgcctg aaggcggagg atctggtcga ggcggagacg gcccgcttcc gctcgttcga
     2341 caacaccgag gtccccggga tcctgtacaa gccgcaccgg gcgtccgcct cgtcgaaggc
     2401 gccggccctc gtctgggtgc acggcggccc cggcgggcag tcgcgcgtca cctacagcgg
     2461 gctcatccag tacctggtca accacggcta cacggtctac gccatcaaca accgcggatc
     2521 gagcggctac ggccgcacct tctacaagat ggacgatcgc aagcacggcg agggggacct
     2581 cgacgactgc gtcgccagca agaagttcct cgccgccacc ggctacgtcg acccggcgcg
     2641 catcggcatc atcggcggct cgtacggcgg ctacatgacc ctggccgccc tgacgttccg
     2701 gccgcaggag ttcgccgtcg gcgtggacat cttcggcgtc tccaactggg tgcggacgct
     2761 gcagagcatt ccgccgtact gggagtcgtt ccgcaaggcg ctgtacacgg agctcggcga
     2821 tccctcgacc gacgaggcgt acctgcgcaa gatctcgccg ctcttccacg cctcggagat
     2881 ccgcaagccg ctcctggtcc tgcagggggc caacgaccca cgcgtgctga aggtcgagtc
     2941 ggacgagatc gtcgccgccg cgaagaactc cggggtgccg gtggagtatc tggtgttcga
     3001 ggacgaagga cacgggttcc gcaagaaaga aaaccagaac aaggggtacc gcgccatcgt
     3061 cgagttcctc gacgtgcacc tcaagggggc gaagccgaag gggccggacg gcgtgacccc
     3121 ggcgaccacg gccgaagccc cggccgccgc cgcacccgtc gccgcgcccg ccgcggcgcc
     3181 cgaaaccggg gacgcggccc gcaagtagag gccgggagcc gccggcatcg aatgaccatc
     3241 gtcgatgtcg cagtcgcggc gatcgccggc gccgtcctgc tcctgctcgg cgggatcgcc
     3301 ctggcagtct tccaggagtc gcgccccgat cgggtgccgg cgctcctcta ccaccgcttc
     3361 ctgcaccccg ggctccgcga ccaggcgcgc gcgctgggcg tcgatctcag ctacgtgacc
     3421 ttcgcggacg cgttcgacga gcagatgggg ttcctcgagc gcgccgggta cgcgccgatc
     3481 accctgaggg acttcgtcgc gtggcagcga ggcgaggcgg cactgccggc gaagccgatc
     3541 ctcatcacct gcgacgacgg gttcgagagc gtccaccggt tcgcctggcc cgtctggcgg
     3601 gcccgccgga tgaaggcgac ggtgttcgtg acccccgacc gggaggcatg gaacttccgc
     3661 aagcactcgg ccctcgacgg tccgctcacc cccgcgcagg tgcgcgagat ggcggccggc
     3721 ggcgtggcga tcgaatccca cggcatgacc cacgcgtacc tgtcgcgcct gtcccgggat
     3781 caggtcctgt gggagctgcg cgagtcgaag cgggtcctcg aagccctgcg gggagagccg
     3841 gtgcgctttt tcgccgttcc cgggggggat tacgacgcgc ggctgcgcgg ccttctccag
     3901 gaggcgggtt acgaggcgtc gttctgcgtc ctcaagggga ccaaccgccg cggcagcgac
     3961 ccgtaccggc tgcggcggct gaccgtcggg ccggaccatc gtctcgagga cttcgcgcgg
     4021 ctgctgcatc ccgcgacctg ctggaagctg aggctcgtga gcacctgcca cgagtggatg
     4081 acgcgggtgc tggggccgca gcgctccgaa aggttcgagg tggcgttgaa gcggctcggc
     4141 gcgcgccgcc tgttcctgcc cggggcgcag gcccgtctgt ggaccgcggc gatcctggtc
     4201 ctggggctga ccctcgtgct cctggcggcg tgggtggtcc tgcgttagcg cgggccggtc
     4261 aggtgcagat gtagtcgtcc agccaggcgc ggttgcgatc gaccaccatc ccggtcgtgg
     4321 cgcagttgag gcagcggcac agaccgggcc cccgcggcac gcggtaggag agatcccgcg
     4381 acttcggatc tccgagaatc ggctcgaggc gggtcatgtc gcgattgccg gcgaggaagc
     4441 gccgcagcgc gacgaggtac ggctcgttgc ggtcgcggcc catgagatcg gcctccggcc
     4501 ggaacgaggg ctgcatcggc tcggtgtccc cccacgcatt gacgcacagc ttgcggacac
     4561 cgttccagcc gatcgtccag gtcgtgtact ggaagaagca gggatgccct tcgtaatcgg
     4621 cggcgcgccg ctcgcggtgc gcctccgggt ggaccatctt caggagcagc cggtccgccc
     4681 cgagcgactc cgccagcgcc ggcatgcgat ccgccaggtg ccggttgtgg tcgaagccga
     4741 tgaactgcac ttcgatgagc gggttggagc gccgcgcggc ggcccgcgcc gccaccagct
     4801 cgcggacgtg ctccttcacc gtctcgagcc ggccgccgac gcggtacttc tcgtacgact
     4861 cctgatccgg accgtcgatg gagacgatga tgtagtcgag cccggcctcc acgagccggc
     4921 gggcctggcc ggcggcgagc gtcttctccc cgaagtgcgt gctgatcgcg gtggccgacc
     4981 cgcgatcgtg cgccagggcg atcagatcgg cgatctgcgg atgcagtagc ggctcgccga
     5041 gcccgtcgag gcgcgtgatc agcaggttcg gtgcgaccgc gtccagcacg cggcggagct
     5101 cctccggcgc caggaacccc ttcggcgtcg gattgatgcg cagcccggtc gcgcagccgg
     5161 ggcaacggag attgcaggta ttggtcgctt cgatgcggag gatgtacggc gtcgatcgga
     5221 tcaccggacg gcgcagcgcc atctccgcgt ggcagagcag cgcgttggcg accttgcggg
     5281 gcgtggcgta agggctcagg acgcgccagt agcgcgcggc ctggttcagg atgctgccgg
     5341 gcgcccggtg gaaccgcttg cccggggccc cccgattgag aagaccctgc agtctcgatc
     5401 gcatcgtgcg tccgtactct ggcggcggag gttctgcagg actccagata cgacgcgacc
     5461 gccccgtggt caaggtcgat cgcgacccgg cccccctcct ttcctcagcc gccgtgatgg
     5521 aacagccggc gccggttcct cagcaggtac cagacgatga cggcgttgac gaccaggatc
     5581 acggcgatcg tccccgagaa ccggcggacg agctcgaaca cctcgatcgg gatgaagaac
     5641 gcggtctcgc cgatggccag ccaggcggcc caggaggccc gccgcatcag cccggcgctc
     5701 tccaccatga ggaggaggcc gtagaggaag gtgccggagg cgaggaagcg gatgcgttcc
     5761 ggcgtgatcg tcaacaggcg ctcgccgagg ctggcgaaga aggtgtgctc cggatccatc
     5821 cgcacgaagc gcaggaagcg atcgagctcg acgcgcagat cgttgtccat gagcgagtag
     5881 atgccgaagg cgaggccgag gagcagggcg cacttcccca gcttgagggc gacgatggtg
     5941 tagagcccgg ccgccggctt cgccgtcagc ccggtctccc cgttcccggc acgacgctgg
     6001 tgagatccct cagaacttga ccttgggggt ctcccgcgtg gcggcgtccg cttcgaagta
     6061 gtccttttcg ccgaagctga agaggttggc gacgatgttc cccgggaagc gcttgatgag
     6121 cgtgttgtag ccgcgcaccg cctcgttgta gcgcatccgc tcggtcgcga tgcggttctc
     6181 gctgccggcc agctcgtcct gaagccggat gaagttctcg ttcgccttga gctggggata
     6241 gttctcggcg atcgccagga gccggccgag ggccgaggtg aggccctggt tggcggccgt
     6301 cgcctcctgc ggcgtcgtcg cccccgcgag gcggctgcgc gccgcggcga cgttctcgaa
     6361 gacttccttc tcgtgcgccg cgtacccctt gaccgtctcg acgag
//
LOCUS       DASRIQ010000576         8637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3902924, whole genome shotgun sequence.
ACCESSION   DASRIQ010000576 DASRIQ010000000
VERSION     DASRIQ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3902924"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /gene="gpmI"
                     /locus_tag="VFD06_14265"
     CDS             <1..1415
                     /gene="gpmI"
                     /locus_tag="VFD06_14265"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZN04744.1"
                     /translation="LEASGEAVGLPPGFIGNSEVGHLCLGAGRVVFQDLQRIQRAIAD
                     GSIQRNAVLLQAMAEASRPGAALHVMGLWSDGGVHSHLDHFDSIVDLARARGVSTLFA
                     HAFLDGRDTPPQSAVPYLDRGEERLKKNGYAPIATVSGRYYAMDRDQRWERTERAWRA
                     LALREGEKAASAVESVRAAYARGVGDEFVEPAIVAPVPGAARTAAVRDGDVVLFFNFR
                     ADRARQITRSFTERGFDRFHRTSPPMLSRFVCWTTYDRTWSLPVAFPPHSIRNTFGEV
                     AAAAGIPQLRIAETEKYAHVTYFFNGGVEPPFPLEERCLIPSPKVATYDLKPEMSARE
                     LTAETVRRLQAKPRQFVLLNYANADMVGHTGKLQATVEACRVVDQGVEAVVTAILALG
                     GVAIVTSDHGNAEQMIDPVTFGPVTAHSMNTVPVHVVGPGVKGRRLRPSGLLADVAPT
                     MLEILGLPVPREMEGRSLLL"
     gene            1426..2142
                     /locus_tag="VFD06_14270"
     CDS             1426..2142
                     /locus_tag="VFD06_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZN04745.1"
                     /translation="MFVKVRTLFGAVLGTVLPADCLVCERLLPWSQEGGVCPACWDRL
                     PWAPRLLRRAACRPEAGGLWGIAAAIVYRDAARRLVHALKFERFDPLGPALGRRAAER
                     SAALLGLLPPADVIVPVPLHWTRRFQRGFNQAEHLARGVARATGLPCEPRLLTRVRRG
                     RRQRGLGHRARRGGFAGVFAAAPAARGARVLLVDDVVTTGTTIEACAAALGIAGTRTV
                     SGFCVARTPRLRSFPETSLR"
     gene            2338..8052
                     /locus_tag="VFD06_14275"
     CDS             2338..8052
                     /locus_tag="VFD06_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZN04746.1"
                     /translation="MPNTETTTPLARGDEVVTAVPPSKLHDLLAGLQAARDGDFSFRL
                     PGHWTGLEGKIADTFNEIVASNAQIATELERVGSLVGKHGQTRQRVQFKEAAGSWGQM
                     ESSVNTLIDDLLWPTTEVTRALAGVAKGDLLQTVRLDVDGRPLEGEFMRSATIVNTMI
                     QQLSVFTSEVTRVVREVGTDGKLGGQAQIRDVSGVWKELTDSVNSMASNLTAQVRNIS
                     EVTIAVANGDLSRKITVDVRGEILQLKEAINTMVDQLRSFASEVTRVAGEVGTDGRLG
                     GQAVVPGVAGMWKDLTDSVNAMARNLTDQVRNIAEVTTAVARGDLSRKITVDVRGEIL
                     ELKNTINTMVDQLNAFASEVTRVAREVGTDGKLGGQAQVPGVAGTWKDLTDNVNFMAS
                     NLTAQVRNIADVATAIANGDLSKKITVNVSGEILLLKETINTMVDQLNAFAAEVTRVA
                     REVGTEGMLGGQAQVPDVGGTWKDLTDSVNAMAGNLTVQLRDVSKVATSIAHGDLSQK
                     ITVNVSGEILQIKDTFNTMVDQLNAFAGEVTRVAREVGTEGRLGGQANVVGVAGTWKD
                     LTDSVNSMANNLTAQVRNIADVATAIARGDLSRKITVDVKGEILELKNTINTMVDQLN
                     AFAAEVTRVAREVGTEGKLGGQAEVPGVAGTWKDLTDNVNFMASNLTSQVRNIAGVAT
                     AIAKGDLSKKITVDVRGEILLLKETLNTMVEQLRSFAAEVTRVAREVGTDGRLGGQAV
                     VPGVAGTWKDLTDNVNLLAANLTTQVRNIAEVTTAVARGDLSRKITVDVKGEILELKN
                     TINTMVDQLNAFAAEVTRVAREVGTEGKLGGQAKVSGVAGTWKDLTDNVNVMASNLTE
                     QVRGIVKVVTAVADGDLQQKLTVKAKGEVAALAETINNVTGTLATFADQVTSVAREVG
                     VEGRLGGQANVPGAAGTWKDLTGNVNLLAANLTTQVRAIAEVATAVTKGDLTRSIQVN
                     ARGEVAELKDNINTMIDNLRLTTDRNTEQDWLKTNLARFTGMLQGQRDLATVGQMLLT
                     ELVPLVNGHQGVIYQMVEEPEPSLGMLASYALDRDRSYPSRLAVGEGFIGQCARDKRR
                     ILITQVPDDAVPVGTVFFKALPRNIAVFPVVFEGQIKAVLGLASLKEFSPTHVAFLEQ
                     LTASIGIVLNSIEATMQTEGLLKQSQQLAGELQTQQKELQQTNEQLAQKAQQLAEQNA
                     EVERKNQEIEQARRALEEKASELALTSKYKSEFLANMSHELRTPLNSILILGQQLSDN
                     PQGNLTEKQVEFARTIHGAGTDLLNLISDILDLSKIESGTVTVESEDVPFLNLVDMVQ
                     RTFRHEAEGRGLTFDIDLDPRLPRSLVSDSKRVQQVLKNLLSNAFKFTPKGGVRLRVF
                     SAAGGWTPSHPVLSSMERAVAFEVTDTGIGIPAEKQRIIFEAFQQADASTSRKFGGTG
                     LGLAISRELSTLLGGELQLRSTPGVGSTFTLYLPLKYAPPSVEIERSLPALPPPRLAV
                     VKAAEPVEEVPDDRDSIQPNDATMLVIEDDTHYARVLADLARARGFKVLIALRGADAL
                     ALARQYRPSAITLDVFLPDVLGWTVLSQLKQDPLTRHIPVQVVTLDEDRQHGLARGAF
                     SFLTKPATTDRLESALDRIQAYTVSRRRRLLVVEDNTAEQASIRELLGHDDIDIVTAG
                     TGAEAFDVLKAQPCDCIVLDLRLPDMSGFEILDRFRLDPALSEIPVVVFTGREMTPEE
                     EAQLREMARSVVVKGVESPERLLDETSLFLHRVWTDLPPEKQRMLERLYNSDEHLVNK
                     KVLVVDDDVRNIFALSSALERRGMRVFTASTGGEAIALLKMDPEISIVLMDIMMPAMD
                     GYQTMQAIRRNPLFRRLPIVALTAKAMKGDREKCLQAGASDYLAKPVNTDQLFAALRM
                     WLHR"
     gene            8088..>8637
                     /locus_tag="VFD06_14280"
     CDS             8088..>8637
                     /locus_tag="VFD06_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN04747.1"
                     /translation="MAEPVNILLVDDQPAKLLTYETILSELGENLVTASSGREALECL
                     LKMDIAVVLIDVCMPELDGYELASLIRQHPRFKETSILFVSATLMTDFDRLRGYEVGG
                     VDYLPVPIVPEILRAKVRVFVDLYRKTRQLETLNKELERRVAERTSALEATAAALQAS
                     DQRKDEFLATLAHELRGPLAPLS"
BASE COUNT         1423 a         3095 c         2912 g         1207 t
ORIGIN      
        1 agctcgaggc gtccggcgag gcggtcggcc ttccccccgg cttcatcggc aattccgagg
       61 tcgggcacct ctgtctcggc gccggacgcg tcgtcttcca ggacctgcag cggatccagc
      121 gggcgatcgc cgatggctcg atccagagga acgccgtgct gctgcaggcg atggccgagg
      181 cgtcgcgccc cggcgccgcg ctgcacgtca tggggttgtg gagcgacggc ggcgtgcaca
      241 gccatctcga ccacttcgac tccatcgtcg acctggcgcg cgctcgcggg gtctccaccc
      301 tgttcgcgca cgccttcctg gacgggcgcg acaccccgcc ccagagcgcc gtgccgtacc
      361 tcgatcgcgg cgaggagcgc ctgaagaaga acggctacgc gccgatcgcc acggtctccg
      421 ggcgctacta cgccatggac cgcgaccagc gctgggagcg gaccgagcgg gcctggcggg
      481 ccctggcgct gcgcgagggg gagaaggcgg cgtccgcggt cgagtcggtg cgcgccgcgt
      541 acgcgcgcgg ggtcggcgac gagttcgtcg agccggcgat cgtcgcgccg gttccgggcg
      601 ccgcccgcac ggcggccgta cgcgacggcg acgtcgtcct cttcttcaac ttccgggccg
      661 accgcgcgcg ccagatcacc cgctcgttca ccgagcgcgg cttcgatcgg ttccaccgca
      721 cgtcgccgcc gatgctgtcc cgcttcgtct gctggacgac ctacgatcgg acgtggtcgc
      781 tgccggtcgc cttcccgccc cattcgatcc gcaacacgtt cggcgaggtg gcggcggccg
      841 cggggatccc gcagctccgc atcgccgaga ccgagaagta cgcgcacgtg acctacttct
      901 tcaacggcgg ggtcgagccc cccttccccc tcgaggagcg ctgcctcatc ccctccccca
      961 aggtcgccac ctacgatctg aagccggaga tgagcgcccg cgagctgacc gccgagacgg
     1021 tgcgccgcct tcaggcgaag ccgcgccagt tcgtgctgct caactacgcc aacgccgaca
     1081 tggtgggcca caccgggaaa ctgcaggcca ccgtcgaagc ctgccgcgtc gtcgaccagg
     1141 gagtcgaggc agtggtgacg gcgatcctgg ctctcggcgg cgtggcgatc gtcacctccg
     1201 atcacggcaa cgccgagcag atgatcgatc cggtgacctt tggcccggtg acggcgcact
     1261 cgatgaacac ggtgccggtg cacgtcgtcg ggccgggcgt gaagggccgc cggctgcggc
     1321 cgtcggggct gctcgccgac gtcgcgccga cgatgctcga gatcctgggc ctgccggtcc
     1381 cgcgcgagat ggagggccga tccctgctcc tctagcgggg gccggttgtt cgtcaaagtc
     1441 cgaacgctgt tcggcgccgt cctggggacg gtgctccccg ccgactgcct ggtgtgcgaa
     1501 cggctcctgc catggagcca ggaggggggc gtctgccccg cctgctggga tcgcctgccg
     1561 tgggcgcccc ggctgctccg gcgcgccgcg tgccggccgg aagcgggcgg cctctggggg
     1621 atcgcggcgg cgatcgtcta tcgggacgcg gcgcgccgcc tcgtgcacgc gctgaagttc
     1681 gagcggttcg accctctcgg gccggcgctc gggcgccgcg cggcggagcg ctccgcagcg
     1741 ctgctcggcc tcctgccgcc ggccgatgtg atcgttccgg tgccgctcca ctggacgcgc
     1801 cggttccagc gcggtttcaa ccaggcggag cacctggccc gcggcgtcgc ccgcgcgacc
     1861 gggcttccct gcgagcctcg actgctcacg cgcgtccgtc gcggccggcg ccagcgcggg
     1921 ctcggtcacc gggcgcgccg cggcggcttc gcgggggtgt tcgcggccgc gcccgcggcg
     1981 cgcggcgcgc gcgtactgct ggtcgacgac gtggtcacca ccgggaccac gatcgaggcc
     2041 tgcgccgcgg cgctcgggat cgccggaacc cgcacggtga gcggcttctg cgtggcccgc
     2101 actccgcgcc tgcgtagttt tccggagacc tctctacgat gaccctgtgg caaacttttc
     2161 cgagttcgaa ccaggggggg tacggagtgt tatccgccgg catccgcgca cgatcatgac
     2221 gattcaatct ccctccccgc ttcaggcgcg acgcctctcg tccgacgccc cctcgtgatc
     2281 gaccctctcg accggcccgc caatcccgcg ggaggcccga ccgtctccgg agccaccatg
     2341 cccaacaccg aaaccaccac tccgctcgcg cggggagatg aggtcgtcac ggcggtcccg
     2401 ccgtcgaagc tgcacgatct cctggccggc ctgcaggcgg cgcgcgacgg ggatttctcc
     2461 ttccggctcc cgggacactg gaccggcctg gaggggaaga tcgccgacac cttcaacgag
     2521 atcgtcgcgt cgaacgcgca gatcgccacc gagctcgagc gggtcggcag cctcgtcggc
     2581 aagcacgggc agacgcgtca gcgggtgcag ttcaaggagg cggccggctc gtgggggcag
     2641 atggagtcga gcgtcaacac gctcatcgac gacctcctgt ggccgacgac cgaggtgacg
     2701 cgggcgctgg ccggcgtcgc caagggggac ctgctccaga ccgtcaggct cgacgtcgac
     2761 ggccgccccc tcgaggggga gttcatgcgc tcggcgacga tcgtcaacac gatgatccag
     2821 cagctgagcg tcttcacctc cgaggtgacg cgcgtcgtgc gcgaggtcgg cacggacggg
     2881 aagctcggcg gccaggcgca gatccgcgac gtctcaggcg tctggaagga gctgaccgac
     2941 agcgtcaact cgatggccag caacctgacg gcgcaggtgc gcaacatctc cgaggtgacc
     3001 atcgccgtcg ccaacggcga cctgtcgcgg aagatcaccg tcgacgtccg cggcgagatc
     3061 ctgcagctga aggaagcgat caacaccatg gtcgaccagc tgcgctcgtt cgcgtcggag
     3121 gtgacgcgcg tcgcgggcga ggtcggcacc gacgggcgtc tcggcggcca ggcggtcgtg
     3181 cccggggtcg ccggcatgtg gaaggacctc accgactcgg tcaacgccat ggcccgcaac
     3241 ctgaccgatc aggtgcgcaa catcgccgag gtgacgacgg ccgtcgcgcg cggcgacctg
     3301 tcgcgcaaga tcaccgtcga cgtccgcggc gagatcctcg agctgaagaa caccatcaac
     3361 acgatggtgg accagctcaa cgccttcgcg tcggaggtca cgcgcgtcgc gcgcgaggtc
     3421 ggcaccgacg ggaagctcgg cggccaggcg caggtgcccg gcgtcgccgg cacctggaag
     3481 gacctcaccg acaacgtcaa cttcatggcc tcgaacctga cggcgcaggt gcgcaacatt
     3541 gccgacgtcg ccaccgccat cgccaacggc gacctttcca agaagatcac cgtcaacgtc
     3601 agcggcgaga tcctgctcct caaggagacc atcaacacga tggtcgatca gctcaacgcc
     3661 ttcgccgccg aggtcacccg cgtggcgcgc gaggtcggca ccgaggggat gctcggcggc
     3721 caggcgcagg tgccggacgt cggcggcacc tggaaggacc tgaccgactc ggtcaacgcc
     3781 atggccggca acctcaccgt gcagctgcgc gacgtctcca aggtcgcgac ctccatcgcc
     3841 cacggcgacc tctcccagaa gatcaccgtc aacgtcagcg gcgagatcct ccagatcaag
     3901 gacaccttca acacgatggt ggaccagctc aacgcctttg ccggggaggt cacccgcgtc
     3961 gcgcgcgagg tcggcaccga ggggcgcctc ggcggacagg cgaacgtcgt cggcgtcgcc
     4021 gggacgtgga aggacctgac cgactcggtc aactcgatgg ccaacaacct gaccgcgcag
     4081 gtgcgcaaca tcgccgacgt ggccaccgcc atcgccaggg gggacctgtc gcgcaagatc
     4141 accgtcgacg tgaaagggga gatcctcgag ctgaagaaca ccatcaacac gatggtcgat
     4201 cagctcaacg ccttcgccgc cgaggtcacc cgcgtcgccc gcgaggtcgg caccgagggg
     4261 aagctcggcg gccaggcgga ggtgcccggc gtcgccggca cctggaagga cctcaccgac
     4321 aacgtcaact tcatggcctc gaacctgacc tcccaggtgc gcaacatcgc cggcgtcgcc
     4381 accgccatcg ccaaggggga cctttccaag aagatcaccg tcgacgtccg tggcgagatc
     4441 ctgctcctca aggagaccct caacacgatg gtcgagcagc tgcgctcctt cgccgccgag
     4501 gtcacgcgcg tggcgcgcga ggtcggcacc gacgggcgcc tcggtggcca ggcggtcgtg
     4561 cccggggtcg ccggcacctg gaaggacctc accgacaacg tcaacctgct cgccgccaac
     4621 ctgacgacgc aggtacgcaa catcgccgag gtgacgaccg ccgtcgcccg cggcgacctg
     4681 tcgcgcaaga tcaccgtcga cgtgaagggg gagatcctcg agctgaagaa caccatcaac
     4741 acgatggtgg atcagctcaa cgccttcgcg gcggaggtca cccgcgtcgc ccgcgaggtc
     4801 ggcaccgagg ggaagctcgg cggccaggcg aaggtctccg gcgtcgccgg cacctggaag
     4861 gacctcaccg acaacgtcaa cgtcatggcc tcgaacctga ccgagcaggt gcgcggcatc
     4921 gtcaaggtcg tcaccgccgt cgccgacggc gatctgcagc agaagctgac ggtgaaggcc
     4981 aagggagaag tcgcggcact ggccgagacg atcaacaacg tgaccgggac gctcgccacc
     5041 ttcgccgacc aggtcacctc cgtggcgcgc gaggtcggcg tcgaggggcg cctcggcggc
     5101 caggcgaacg tccctggcgc ggccggaacc tggaaggacc tcaccggcaa cgtcaacctg
     5161 ctcgccgcca acctgacgac gcaggtccgc gccatcgccg aggtggcgac cgccgtcacc
     5221 aagggggacc tgacccggtc gatccaggtc aacgcccgcg gcgaggtcgc cgagctgaag
     5281 gacaacatca acacgatgat cgacaacctc cggctcacga ccgaccgcaa caccgagcag
     5341 gactggctga agacgaacct ggcgcgcttc accggcatgc tgcaggggca gcgggacctg
     5401 gcgaccgtcg gccagatgct cctcaccgag ctggtgccgc tggtcaacgg gcaccagggg
     5461 gtcatctacc agatggtcga ggagccggag ccgagcctcg gcatgctcgc gagctacgcg
     5521 ctggatcggg atcgctccta cccgtcccga ctcgcggtcg gcgaggggtt catcggccag
     5581 tgcgcccgcg acaaacggcg catcctcatc acgcaggtcc cggacgacgc ggtgccggtg
     5641 gggacggtct tcttcaaggc gttgccgcgc aacatcgccg tgttccccgt cgtcttcgag
     5701 ggacagatca aggcggtgct cgggctcgcc tccctcaagg agttctcccc cactcacgtc
     5761 gccttcctcg agcagctgac ggcgagcatc ggcatcgtgc tcaacagcat cgaggcgacg
     5821 atgcagaccg aggggctcct caaacagtcg cagcagctcg ccggtgagct gcagacgcag
     5881 cagaaggagc tgcagcagac caacgagcag ctggcccaga aggcgcagca gctcgccgaa
     5941 cagaacgccg aggtggagcg caagaaccag gagatcgagc aggcgcgccg ggcgctcgag
     6001 gagaaggcct ccgagctggc gctcacctcg aagtacaagt cggagttcct ggccaacatg
     6061 tcacacgagc tgcgcacccc gctcaacagc atcctgatcc tcgggcagca gctctccgac
     6121 aacccccagg ggaacctgac cgaaaagcag gtggagttcg cgcgcaccat ccacggagcc
     6181 ggcaccgacc tcctgaacct catcagcgat atcctcgacc tctccaagat cgagtccggg
     6241 accgtgacgg tggagtcgga ggacgtgccg ttcctcaacc tggtggacat ggtgcagcgg
     6301 acgttccggc acgaggcgga ggggcgcggc ctgaccttcg acatcgacct cgacccccgc
     6361 ctgccgcgca gcctggtgtc cgactcgaag cgcgtgcagc aggtcctcaa gaacctgctc
     6421 tcgaacgcct tcaagttcac gccgaagggg ggcgtgcggc tgcgggtctt ctccgcggcg
     6481 ggcggctgga cgccgagcca tccggtgctg tcgtcgatgg aacgagccgt cgccttcgag
     6541 gtcacggaca ccggcatcgg catcccggcg gagaagcagc ggatcatctt cgaggcgttc
     6601 cagcaggccg acgcgagcac cagccgcaag ttcggcggca ccgggctggg gctggcgatc
     6661 agccgcgagc tgtccaccct cctcggcggc gagctccagc tgcgcagcac tcccggcgtc
     6721 ggcagcacgt tcaccctgta cctgccgctg aagtacgccc cgccctccgt ggagatcgag
     6781 cggagcctgc cggccctgcc gccgccgcgc ctggccgtgg tgaaggcggc cgagccggtc
     6841 gaggaggttc ccgacgatcg ggactcgatc cagccgaacg acgcgacgat gctcgtcatc
     6901 gaggacgaca cgcactacgc gcgcgtcctc gccgacctgg cgcgcgcgcg cgggttcaag
     6961 gtcctgatcg ccctgcgggg ggccgacgcg ctggcgctgg cgcggcagta ccgcccctcc
     7021 gccatcaccc tcgacgtctt cctcccggac gtcctcggct ggacggtcct cagccagctg
     7081 aagcaggacc cgctgacgcg ccacatcccg gtgcaggtcg tcacgctcga cgaggatcgg
     7141 cagcacgggc tggcgcgcgg cgccttctcg ttcctgacca agcctgccac gaccgatcgc
     7201 ctcgaatcgg cgctcgatcg catccaggcc tacacggtct cgcggcgcag gcgcctgctc
     7261 gtcgtcgagg acaacacggc ggagcaggcc agcatccgcg agctgctcgg ccacgacgac
     7321 atcgacatcg tcaccgccgg caccggcgcc gaggccttcg acgtcctgaa agcgcaaccc
     7381 tgcgactgca tcgtcctcga cctgcgcctc cccgacatgt ccggcttcga gatcctcgac
     7441 cggttccggc tcgacccggc gctgtccgag atcccggtcg tcgtcttcac cggccgggag
     7501 atgaccccgg aggaggaggc gcagctgcgc gagatggcgc gatccgtcgt ggtcaagggg
     7561 gtggagtccc ccgagcgcct gctcgacgag acctccctct tcctgcaccg cgtctggacc
     7621 gacctgccgc ccgagaagca gcgcatgctg gagcggctgt acaactccga cgagcacctg
     7681 gtgaacaaga aggtgctggt ggtggacgac gacgtccgca acatcttcgc gctgtcgagc
     7741 gcgctcgagc gccgcggcat gcgggtcttc accgcgagca cgggagggga ggcgatcgcc
     7801 ctgctcaaga tggacccgga gatctcgatc gtcctgatgg atatcatgat gcccgccatg
     7861 gacggctacc agacgatgca ggcgatccgc cgcaacccgc tcttccgccg gctgccgatc
     7921 gtcgccctca ccgccaaggc gatgaagggg gaccgggaga agtgcctgca ggcgggagcc
     7981 tcggactacc tcgccaaacc ggtcaacacc gaccagctgt tcgccgcgct gcgcatgtgg
     8041 ctccatcgct gaggggaacg gctccgcatc catgggagga gaacgccttg gctgagccgg
     8101 tcaacatcct gctggtcgac gaccagccgg cgaagctcct gacctacgag acgatcctct
     8161 cggagctcgg ggagaacctg gtcacggcgt cgtcggggcg cgaagcgctc gaatgcctgc
     8221 tgaagatgga catcgcggtc gtcctcatcg acgtctgcat gcccgagctc gacggctacg
     8281 agctggcgtc gctcatccgc cagcacccgc gcttcaagga gacctcgatc ctcttcgtgt
     8341 cggccaccct gatgaccgac ttcgaccggc tgcgggggta cgaggtcgga ggggtggact
     8401 acctgccggt gccgatcgtg ccggagatcc tgcgcgccaa ggtgcgcgtc ttcgtcgatc
     8461 tgtaccgcaa gacccgccag ctcgagacgc tgaacaagga gctggagcgg cgggtggcgg
     8521 agcgaacctc ggcgctcgag gcgaccgccg ccgcgctgca ggcctccgac cagcgcaagg
     8581 acgagttcct cgcgacgctc gcccacgagc tgcgcggccc gctcgccccc ctgagca
//
LOCUS       DASRIQ010000577         7272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3337432, whole genome shotgun sequence.
ACCESSION   DASRIQ010000577 DASRIQ010000000
VERSION     DASRIQ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3337432"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VFD06_14285"
     CDS             <1..1304
                     /locus_tag="VFD06_14285"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HZN04748.1"
                     /translation="DAFIVRVPSHLLVTGGGGADVSAPVLSAVSLATVGGTNATIAWS
                     SSEPADSQVDYGPSAAYGSSTVRSAAYVTSHSQGLSGLLAGTQYHYRVRSRDAAGNLS
                     LSADQTFTTSLDNPAVGMSAWWTLDEGAGTVIADATGSGHGGLLVNGPARIAGRTGLA
                     VAFDGVNDTASVPHHAALDAYPLSTAFWMKTGATGITALVNKYAASSLNGWQVFTGGG
                     NLCAWYFRNASNYVWDGGGCTMAVAGINDNAWHHVVFVVDATGGRLYVDGALRASQGW
                     TGTPGPATTTQPLTFASYPGTTGPPLAGALDDVRVYGRALGSSEIAALANVPARDLTP
                     PVISQMTARTLLSTSTMMVGWQTDELSDTRLIYGPTAGYGSVTPLTTTPMRSHYVTLG
                     GLMPGTVYHCRAISRDAAGNSTTSPDYVFTTPAAGVTTTTD"
     gene            complement(1314..2483)
                     /locus_tag="VFD06_14290"
     CDS             complement(1314..2483)
                     /locus_tag="VFD06_14290"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN04749.1"
                     /translation="MTAQVLFWGSLLLLLYAYAGYPLVMRLWAQLRPRPVLRRRVEPF
                     VSVLLAAHDEAETIGPRLENLLSLDYPADRLEVLVGSDGSNDRTAARARASGSKRVRV
                     FEFETRRGKPSVLNDLAAQAQGDLLLFADARQRYERHALRAIVAPFADARVGGVGGAL
                     LIGGVGGGGIGQGAAVYRRIDDAIRQDESWVDSTVGLSGAIYAIRAPLFEPLPADAVL
                     DDVLTPMRVTRRGFRVVFEPQARAFDRAPATGEEEFTRKVRTLAGNLHLLARERWLLN
                     PFENRLWFQTLSHKTLRTAGPLLLLLMLLASVWLAPGSPYAVALFGQACFYGAAAAGW
                     LLRDLPPVSRLLGFPYTFCLFNAAAAVAIGRVLFRRQPVTWRKTGTLRPMPPAAG"
     gene            complement(2480..4123)
                     /locus_tag="VFD06_14295"
     CDS             complement(2480..4123)
                     /locus_tag="VFD06_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04750.1"
                     /translation="MSRRPVPALAITALLAAALVSAGARKKPAPPAPAGPPAPAAAVL
                     PRDTVDLAPPQEGRTIDVEAGADLQAALDAASPGDTLVLPAGAVFRGPFDLPKTTGEG
                     WITLRTGGEGQGLPPRGTRVSPDDAPAMAVLESAEGTVLRAGPGTHHVRFVGLEIRPS
                     AGTFLYELVRLGTKETAAGETPHHVVFERCYVHGDPDRGGRRGIALNGRHIAVVDSYL
                     SDFKEKGADSQALCGWNGPGPFLVANSTLEAAGENIMFGGAPPTIPDLVPSDITIVGN
                     LLRKPLAWKAEEPGHDGSSWSVKNLFELKNARRVKVSGNRFENGWAQSQRGFAIVFTV
                     RDEEGKAPWAVVEDVTFSDNVVAGAGNGVNILGRDTSHPGGGGRSARIRIANNVFDDI
                     GGPRWGGKGTLFQILAGASDVVIEHNTGMQTGGIVMAEGGPFVRFVLKDNVVRHNQFG
                     IAGTGTGIGVPTLLAQFPGSIVTGNVIVGGAAGRYPPGNSFPQTVEEVGFVDPGRRNY
                     AIAPTSRVRGAGSDGRTPGANDEVMRRACGEAQTAEGGR"
     gene            complement(4120..5322)
                     /locus_tag="VFD06_14300"
     CDS             complement(4120..5322)
                     /locus_tag="VFD06_14300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN04751.1"
                     /translation="MNLTASLPAPATRTRLQGMPIVCFAHDFEGDPTSKTHIMRLLAR
                     GGSRVLWVNSIGLRRPSVSRGDARRILDKVRRSLRGCREVEPNLFVTSPLAVPLHGVP
                     MADRFNAALLGSMLRRLCRRLGLERPIVWTFMPNVSGLLGACHERLLVYHCVDEYTAF
                     SGVAKGAIAPMEEDLTRRAAVVFASSERLTSERRPWNANVHFVPHGVDLDHFGRALDP
                     ALPIPEDMRSIPRPIVGFFGLIADWVDLDLVRAAALARPQWSFVLIGKMATTPQALGG
                     VPNVHLLGQKPYAALPGYCRGMDVGIIPFRTSRLTVRANPLKLREYLAAGLPVVSTPL
                     PEVARYDGLVRLAPDLDAFLKAVEAALASRSAEADRARAESMRGESWEERVRDMERIV
                     VQRLGGAR"
     gene            complement(5333..>7272)
                     /gene="asnB"
                     /locus_tag="VFD06_14305"
     CDS             complement(5333..>7272)
                     /gene="asnB"
                     /locus_tag="VFD06_14305"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN04752.1"
                     /translation="GGTHEVLLMCGIAGILKIDPRARAEEPRLQRMLQSLRHRGPDGE
                     GTYVEDGIALGHRRLAIVDPRGGRQPMTNEGSTAWLVCNGEIYNHPELQARLQGRGHV
                     YRSNSDNETIVHLHEEEGDECVRSLRGMFAFALWDKARGRLLLARDRLGIKPLYYVLT
                     AQELLFASEIKALLAAGVRAEFDESVLPEYLATRFVSGPGTFFKGIRKLLPGRVLRFT
                     RESGARIERYWTPPPPSELTDGTLQEQAVKVRAGLEDSVKRHLMADVPLGVFLSGGID
                     STGIAAIAAREMDQPIQTFGVGFAEEEVNEMSYARLAARAIGARHHEVEVTADQFFES
                     LPNLVWHEDEPIAFDASIPLHILSRLAAEHVKVVLTGEGADELFLGYNRYRVTAWNEA
                     LGELYWRLVPSTLRGRNSPLIKGLPRVVRRYAERSFLGLDPGPRELFCENFAVFPTFL
                     QKHLLTGSRAGLTRDPYEFILRRYEEAQGNSLDRMARADLETYLVELLMKQDQMSMAA
                     SIESRVPYLDHVLVEQVLSLPSRFKLRGWRTKAVLRAALKDLVPRAILERRKAGFPVP
                     LGRWLREARWPVVQEFVLSPRSLRRGYFDPMLLRTLAQEHKSGVADHCSRLWLLINLE
                     IWQRIFLEGESPAAMPIPAAA"
BASE COUNT         1069 a         2617 c         2608 g          978 t
ORIGIN      
        1 aggacgcctt catcgtccgc gtgccatcgc acctcctggt gactggcggc ggcggggccg
       61 acgtcagcgc gccggtgctc tcggcggtgt cgttggcgac cgtcggcggc acgaacgcca
      121 cgatcgcctg gagcagcagc gagccggccg actcgcaggt ggactacggt ccgagcgccg
      181 cgtacggcag ctcgaccgtg agaagcgccg cttacgtcac cagtcactcg cagggactct
      241 cggggcttct cgccgggacg cagtaccact accgggtccg gtcgcgcgac gccgccggga
      301 atctgtccct ctcggccgat cagaccttca cgacctccct cgacaaccct gccgtcggga
      361 tgtccgcctg gtggacgctc gacgaagggg ccgggaccgt gatcgccgac gcgaccggct
      421 ccggtcacgg tgggctcctc gtcaacgggc cggcacgcat cgccggccgg accgggctcg
      481 ccgtggcctt cgacggagtc aacgacaccg ccagcgtacc gcaccacgcg gcgctcgacg
      541 cctacccgct gagcaccgcg ttctggatga agaccggcgc gaccggaata accgccctgg
      601 tgaacaagta cgccgcctcg tccctgaacg gctggcaggt gttcaccggc ggcggcaacc
      661 tgtgcgcctg gtacttccgg aacgcgtcga actacgtctg ggacggcggc ggctgcacga
      721 tggcggtcgc cggcatcaac gacaacgcgt ggcaccacgt cgtcttcgtg gtcgacgcga
      781 ccggcggacg gctttacgtg gacggggcgc tgcgcgcctc acagggctgg accggcaccc
      841 ccggcccggc cacgaccacg cagcccctga ccttcgcctc ctaccccggg accaccggcc
      901 cgccgctcgc cggggcgctc gacgacgtcc gcgtttacgg ccgcgccctg gggagctccg
      961 agatcgccgc gctggcgaac gtcccggcgc gcgacctgac gccgcccgtc atcagccaga
     1021 tgacggcgcg cacgctcctg tcgaccagca cgatgatggt gggctggcag accgacgagc
     1081 tgtcggacac gcggctgatc tacgggccga cggcgggcta tggctccgtg acgccgctca
     1141 cgaccacacc gatgcgctcg cactacgtga cgctcggtgg cctgatgccg gggaccgtgt
     1201 accactgtcg cgccatctcg cgcgacgccg ccgggaactc gaccacctcc cccgactatg
     1261 tcttcacgac ccccgccgcc ggcgtcacga cgacgaccga ctaggggcct cgatcacccg
     1321 gcagccggag gcatcggtcg gagggtccca gtcttcctcc aagtgaccgg ctgccgccgg
     1381 aacaggacgc ggccgatcgc cacggcggcg gcggcgttga acagacagaa ggtgtagggg
     1441 aagccgagca gccgcgaaac gggcggcagg tccctcagca gccagccggc cgccgcggca
     1501 ccgtagaagc aggcctgacc gaacagcgcc acggcgtagg gcgagccggg ggccagccag
     1561 acgctcgcga ggagcatcag cagcagcagg agcgggccgg ccgtgcgcag cgtcttgtgc
     1621 gacagggtct ggaaccacag ccggttttcg aacgggttga gcagccaccg ctcccgggcc
     1681 agcagatgga ggttcccggc gagcgtccgc accttgcgcg tgaactcctc ctcacccgtc
     1741 gcgggggcgc ggtcgaaggc gcgcgcctgc ggctcgaaca ccacccggaa gccgcgccgg
     1801 gtcacccgca tcggcgtcag cacgtcgtcg aggaccgcgt ccgccgggag cggctcgaac
     1861 agcggggccc ggatggcgta gatcgccccc gacaggccca cggtggagtc cacccaggac
     1921 tcgtcctgac ggatggcgtc gtcgatgcgg cgatagacgg cggccccctg gccgatcccg
     1981 ccgcccccga cgcccccgat caggagcgcc ccgccgaccc cgccgacccg ggcgtccgcg
     2041 aacggcgcga cgatcgccct gagcgcgtgg cgctcgtagc gctgccgcgc gtcggcgaag
     2101 aggagcagat cgccctgtgc ctgcgccgcg aggtcgttga gcaccgacgg cttgccgcgc
     2161 cgcgtctcga attcgaagac ccgcacccgt ttcgaccccg aggcgcgggc ccgcgcggcc
     2221 gtccgatcgt tcgagccgtc cgacccgacg agcacctcca ggcgatccgc cgggtagtcg
     2281 aggctcagga ggttctccag gcgcgggccg atcgtctctg cctcgtcgtg cgccgccagc
     2341 aggacgctca cgaacggctc gacccggcgg cgcagcaccg gccgcggccg cagctgcgcc
     2401 cacagtcgca tgacgagcgg gtagccggcg taggcgtaca ggagcaggag cagcgacccc
     2461 cagaagagga cctgcgcggt catcgccccc cctcagccgt ctgcgcttcg ccgcaggcgc
     2521 ggcgcatcac ctcgtcgttc gcccccgggg ttcgcccgtc gctccccgcg ccgcgcacgc
     2581 ggctcgtcgg agcgatcgcg tagttgcggc ggcccgggtc gacgaagccg acctcctcga
     2641 ccgtttgcgg gaagctgttg ccggggggat agcggccggc ggcgccgccc acgatcacgt
     2701 tgccggtcac gatcgagccg gggaactggg cgagcagcgt cggcacgccg atgccggtgc
     2761 cggtgccggc gatgccgaac tggttgtggc ggacgacgtt gtccttgaga acgaagcgga
     2821 cgaacgggcc cccctccgcc atgacgatgc cgccggtctg catcccggtg ttgtgctcga
     2881 tgacgacgtc ggaggccccc gcgaggatct ggaagagcgt ccccttcccg ccccagcgcg
     2941 gcccgccgat gtcgtcgaag acgttgttgg cgatccggat gcgcgccgag cgcccgcccc
     3001 cgccggggtg gctggtgtca cgcccgagga tgttgacccc gttgccggcg ccggcgacga
     3061 cgttgtcgga gaaggtgacg tcttcgacca cggcccacgg cgccttccct tcctcgtcgc
     3121 gcaccgtgaa gacgatggcg aagccgcgct gcgactgcgc ccagccgttc tcgaagcggt
     3181 ttccggagac cttcacccgc cgcgcgttct tgagctcgaa caggttcttc accgaccagg
     3241 acgacccgtc gtgcccgggc tcctcggcct tccacgccag gggcttgcgg aggaggttcc
     3301 ccacgatggt gatgtcggag gggacgagat ccgggatggt cggcggggcg ccgccgaaca
     3361 tgatgttctc ccccgccgcc tcgagggtgc tgttggccac gaggaacggc ccggggccgt
     3421 tccagccgca cagcgcctgg gagtcggccc ccttttcctt gaagtcggag aggtaggagt
     3481 cgaccaccgc gatgtgccgg ccgttgagcg cgatgccgcg ccgtccgccg cgatcgggat
     3541 cgccgtggac gtagcagcgc tcgaagacga cgtgatgcgg cgtctccccg gccgcggtct
     3601 ccttcgtccc cagccgcacc agctcgtaga ggaacgtgcc ggccgacggg cggatctcca
     3661 ggccgacgaa gcggacgtgg tgcgtgccgg ggccggcgcg caacaccgtc ccctccgccg
     3721 actcgagcac cgccatcgcg ggcgcgtcgt ccggcgacac gcgcgtgccg cgcggcggca
     3781 ggccctggcc ctcgccgccg gtgcgcaggg tgatccatcc ctcgcccgtc gtcttcggca
     3841 ggtcgaacgg gccgcggaag accgcaccgg cgggaaggac gagcgtgtcg ccgggggagg
     3901 cggcgtcgag cgccgcctgc agatccgcgc cggcttcgac gtcgatcgtc cggccttctt
     3961 gaggcggagc gaggtcgacc gtgtcgcgcg gcagcaccgc cgcggcgggc gcgggcgggc
     4021 cggcgggggc ggggggggcc ggcttcttgc gggcgccggc cgacacgagc gccgccgcga
     4081 gcagggccgt gatcgccaac gccggaaccg gccggcggct catcgcgccc ctcccagccg
     4141 ctgcacgacg atccgctcca tgtcgcgcac gcgctcctcc cacgactcgc cgcgcatcga
     4201 ttcggcgcgg gcccggtcgg cctcggccga gcgcgaagcg agcgccgcct cgaccgcctt
     4261 gaggaacgcg tcgaggtccg gcgcgaggcg caccagcccg tcgtagcgcg ccacttcggg
     4321 gagcggcgtc gacaccaccg gcaacccggc ggccaggtac tcgcgcagct tgagggggtt
     4381 ggcccgcacc gtgagccggc tggtgcggaa cgggatgatg ccgacgtcca taccgcggca
     4441 gtagcccgga agcgccgcgt acggcttctg accaaggaga tgcacgttcg gcacgcctcc
     4501 gagcgcctgc ggagtggtcg ccatcttccc gatgagcacg aaggaccact ggggtcgcgc
     4561 cagcgccgcg gcgcgcacca ggtcgagatc gacccagtcg gcgatgagac cgaagaagcc
     4621 gacgatcggg cgcggaatcg accgcatgtc ctcggggatc ggcagcgccg ggtcgagcgc
     4681 gcgcccgaag tgatcgagat cgacgccgtg cggcacgaag tggacgttcg cgttccacgg
     4741 cctccgctcg gaggtcagcc gctcggagga ggcgaacacc accgcggccc ggcgcgtcag
     4801 gtcttcctcc atcggggcga tcgccccctt cgccacgccg gagaaggcgg tgtactcgtc
     4861 cacgcagtgg tagacgagca ggcgctcgtg gcaggcgccg aggagccccg agacgttcgg
     4921 catgaaggtc cagacgatgg ggcgctcgag gccgaggcgc cggcacaggc gccgcagcat
     4981 cgagccgagg agggcggcgt tgaagcggtc ggccatcggc acgccgtgca gcggcaccgc
     5041 cagcgggctc gtgacgaaca ggttcggctc cacctcgcga cagccgcgca ggctccgccg
     5101 caccttgtcg aggatccggc gggcgtcgcc gcgcgacacg gagggccggc gcaggccgat
     5161 ggaattcacc cacagcacgc ggctgccgcc gcgcgcgagc agccgcatga tgtgcgtctt
     5221 gctcgtcggg tccccttcga agtcgtgggc gaagcagacg atcggcatcc cctggaggcg
     5281 ggtccgggtg gcgggagcag gcagcgaggc agtcaggttc atcggtcggc gttcacgcgg
     5341 cggccgggat cggcatggcg gccggcgact ctccttcgag gaagatccgt tgccagatct
     5401 cgaggttgat gagcagccag aggcggctgc agtggtcggc gacccccgac ttgtgttcct
     5461 gggcgagggt cctgaggagc atcgggtcga aatagccgcg gcgcagggat cgcggcgaca
     5521 gcacgaactc ctgcaccacc ggccagcgcg cctcgcgcag ccagcgcccc agcggcaccg
     5581 ggaagccggc cttgcggcgc tcgaggatgg cgcgggggac gagatccttg agggcggcgc
     5641 gcaggaccgc cttggtgcgc cagccgcgca gcttgaagcg ggacggcagc gacaggacct
     5701 gctccaccag cacgtggtcg aggtacggca cgcggctctc gatggaggcc gccatgctca
     5761 tctggtcctg cttcatgagc agctcgacga gataggtctc gagatcggcg cgcgccatcc
     5821 ggtcgaggct gttcccctgc gcctcctcgt agcgacgcag gatgaactcg tacggatcgc
     5881 gggtgagccc cgcccgagac cccgtcagga gatgcttctg cagaaacgtc gggaagaccg
     5941 cgaagttctc gcagaacagc tcgcgcggcc ccgggtcgag gccgaggaag gagcgctcgg
     6001 cgtagcgccg cacgacccgc ggcagtcctt tgatgagcgg gctgttgcgg ccccggagcg
     6061 tcgacggcac caggcgccag tacagctccc cgagcgcctc gttccaggcg gtgacgcggt
     6121 agcggttgta gccgaggaac agctcgtccg ccccctcgcc ggtgagcacc accttgacgt
     6181 gctcggcggc caggcgcgac aggatgtgca gcgggatgct ggcgtcgaag gcgatcggct
     6241 cgtcctcgtg ccagacgagg ttcggcagcg actcgaagaa ctggtcggcg gtgacctcga
     6301 cctcgtggtg tcgggcgccg atcgcccgcg ccgcgaggcg ggcgtacgac atctcgttca
     6361 cctcctcctc ggcgaagccg accccgaagg tctggatcgg ctggtccatc tcccgcgcgg
     6421 cgatggccgc gatgccggtc gagtcgatcc cgcccgagag gaacaccccg agcggcacgt
     6481 cggccatgag gtggcgcttc accgagtcct cgagcccggc gcgcaccttc accgcctgct
     6541 cctgcagcgt cccgtcggtc agctcggacg ggggcggcgg ggtccagtag cgctcgatgc
     6601 gcgccccgct ttcccgcgtg aagcgcagca cgcgccccgg caggagcttg cggatcccct
     6661 tgaagaaggt cccgggaccc gagacgaagc gggtggcgag gtactcgggc aggaccgact
     6721 cgtcgaactc ggcgcgcacc cccgcggcga gcagcgcctt gatctccgat gcgaacagga
     6781 gctcctgagc ggtgaggacg tagtagagcg gcttgatccc gagccgatcg cgcgccagca
     6841 ggagccggcc gcgcgccttg tcccacaggg cgaaggcgaa catgccgcgc aggctgcgga
     6901 cgcactcgtc cccctcctcc tcgtgaaggt gcacgatcgt ctcgttgtcg ctgttgctgc
     6961 ggtagacgtg gccgcgcccc tgcagccgcg cctgcagctc ggggtggttg tagatctcgc
     7021 cgttgcagac gagccaggcg gtcgatcctt cgttggtcat cggctgtcgc ccgccgcgcg
     7081 gatcgacgat ggcgaggcgc cggtgcccga gggcgattcc gtcttcgacg taggtcccct
     7141 cgccgtcggg cccgcggtgc ctgagcgact ggagcatcct ctgcagccgc ggctcctcgg
     7201 cgcgggcgcg cgggtcgatc ttgaggattc cggcgatgcc gcacatcaga agcacctcat
     7261 gagtgccgcc gg
//
LOCUS       DASRIQ010000578         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_762766, whole genome shotgun sequence.
ACCESSION   DASRIQ010000578 DASRIQ010000000
VERSION     DASRIQ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762766"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFD06_14310"
     CDS             <1..376
                     /locus_tag="VFD06_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04753.1"
                     /translation="LLFQVRVQGSLVAVLAVSLLGAISFAGLGLLVACRAQKIETVSG
                     LMNVVMLPMWLLSGIFFSSERFPAALQPVIQALPLTMLNDALRAVILEGAGLASQSLR
                     IAGLVLWGFVSFVLALRWFRWS"
     gene            467..742
                     /locus_tag="VFD06_14315"
     CDS             467..742
                     /locus_tag="VFD06_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04754.1"
                     /translation="MAKTGGGGLGFVMAIVVLVIVLLLAGKAWNAVMPTAQQALTPGA
                     PAGRVDDHGQKEAGDQVRSGGLPNLREMGTSTDQHLRQVQDAEKKQD"
     gene            complement(770..1624)
                     /locus_tag="VFD06_14320"
     CDS             complement(770..1624)
                     /locus_tag="VFD06_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04755.1"
                     /translation="MTTRRRRTSALLPLLLAAGCLLPSGHAQAQESTAVPPAGTAADP
                     HPARTSLKEDPWPAGKRGKYVFWWDHYRDSNGNDFDDFSFFISYRQVYAQVWSGQYTD
                     GIEAGGYVKDRRKSSYGGFARWRDDRDYVAQFDTGQVLGHGLVWVGSLRYIHVIPTTV
                     DDNADDIPDDADGDGIPDPIPGGQNQFQFATGLDWYHGDYDFGTFRAISDPREGGRWT
                     FQLSHRFYFGEPYYIEPGILPRTDGELNWFVRGKIKCFVWLIGDFNQFDFTDVERKQV
                     SLGLEFAY"
     gene            complement(1621..>2564)
                     /locus_tag="VFD06_14325"
     CDS             complement(1621..>2564)
                     /locus_tag="VFD06_14325"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN04756.1"
                     /translation="DDTPKLLAEMKVRSYRTPNMGKVSAVNFGIHRVTTPYVLLLDDD
                     TRVGAAKIPTSLLEKYDAVAFNVVPDRRDRYGPKGSGVLAALQRYEYCKSMEIGRRFQ
                     DATASISCISGAIGLFKRERLQSFHHKHTGVFQGEDLQRTIIELLNGGSVVFADEPVW
                     TVVPDKIADLARQRLTGWYPAHYHMFFSYVRLLFLPSSPWRLRHEMAYNIFVVLTEPL
                     RILSLGLIIYHRLWFELGLLYLLYLLLELLPFTVIQRDFNTGKPLLVLLLYPLFGFVN
                     AVLRVIAWFVWVWKRFVTGEMRRKTEKDRKWNQPATP"
BASE COUNT          423 a          825 c          893 g          423 t
ORIGIN      
        1 gctcctgttc caggtgcgcg tgcagggaag cctcgtcgcc gtgctcgccg tgtcgctgct
       61 cggagcgatt tcgttcgccg gcctcggact cctcgtcgcc tgccgggccc agaagatcga
      121 gacggtctcc ggcctgatga acgtggtgat gctgcccatg tggcttctgt ccgggatctt
      181 cttctcgtcg gagcgcttcc ccgcggcgct gcagccggtc atccaggcgc tgccgctgac
      241 gatgctcaac gacgcgctcc gcgccgtcat cctcgagggg gccggcctcg cgtcgcagtc
      301 gctgcggatc gccgggctcg tcctgtgggg gttcgtgtcg ttcgtcctcg ccctgcgctg
      361 gttccgctgg tcctgattgc atcccgcctg cgcgcgccct actaataagg aagggcgccg
      421 cggaccacgc atcacgaaat cgcgatcggc gccccgggag gggagcatgg cgaagacggg
      481 cggcggcgga ctgggattcg tgatggcgat cgtcgtgctg gtcatcgtgc tgctcctggc
      541 cgggaaggcc tggaacgcgg tgatgccgac ggcgcagcag gcgctcaccc ccggcgctcc
      601 ggccggtcgt gtggacgatc acggacagaa ggaggccggc gaccaggtcc gctcaggggg
      661 cctgcccaac cttcgcgaga tgggcacctc gaccgaccag cacctccgcc aggtgcagga
      721 cgccgagaag aagcaggact gagcgtttcc tgagcggccg cgacgtggac tagtaggcga
      781 actcgagccc gaggctcacc tgcttgcgct cgacgtcggt gaagtcgaac tggttgaaat
      841 caccgatgag ccagacgaag cacttgatct tgccgcgcac gaaccagttc aactcgccgt
      901 cggtgcgcgg caggatcccc ggctcgatgt agtacggctc cccgaaatag aagcgatgcg
      961 acagttggaa ggtccaccgc cctccctcgc gcggatcgct gatggcgcgg aaggtcccga
     1021 agtcgtagtc gccgtggtac cagtcgagcc cggtggcgaa ctggaactga ttctgcccgc
     1081 ccgggatcgg atcgggaatc ccgtcgccgt ccgcgtcgtc cggaatgtcg tcggcgttgt
     1141 cgtccaccgt ggtcgggatg acgtggatgt agcgcaggga gccgacccag acgaggccgt
     1201 gccccagcac ctggccggtg tcgaactgcg cgacgtaatc ccggtcgtcc cgccagcgcg
     1261 cgaacccgcc gtacgacgac ttgcgccggt ccttgacgta cccgcccgcc tcgataccgt
     1321 cggtgtactg gccgctccag acctgcgcgt acacctgcct gtacgagatg aagaaggaga
     1381 agtcgtcgaa gtcgttgccg ttggagtcgc ggtagtggtc ccaccagaag acgtacttgc
     1441 cccgcttgcc ggccggccac gggtcctcct tcagcgaggt cctggcaggg tgcggatcgg
     1501 cggccgtccc cgccggaggc acggccgtgg actcctgcgc ctgagcgtgc ccggacggca
     1561 ggaggcaccc ggccgcgagg agcagcggaa gaagcgcgct cgtccgacgg cgtcgcgtcg
     1621 tcatggggtc gccggctgat tccacttgcg atccttctcc gtcttgcggc gcatctcgcc
     1681 ggtcacgaac cgcttccaga cccagacgaa ccaggcgatg acgcgcagga cggcgttgac
     1741 gaagccgaac agcggataga ggaggagcac gagcaggggc ttgccggtgt tgaaatccct
     1801 ctggatgacc gtgaacggta agagctccaa caacaaataa agcaggtaca ggagtcccag
     1861 ctcgaaccag aggcggtgat agatgatgag cccgagcgac aggatccgga gcggctcggt
     1921 caggacgacg aagatgttgt acgccatctc gtgccgcagg cgccagggcg aagacggcag
     1981 gaacagcagc cggacgtacg agaagaacat gtggtagtgg gccggatacc agccggtcag
     2041 ccgctgccgc gccaggtcgg cgatcttgtc cgggacgacg gtccagaccg gctcgtcggc
     2101 gaagacgacg ctgccgccgt tgagcagctc gatgatggtg cgctgcaggt cctccccctg
     2161 gaagacgccg gtgtgcttgt ggtggaacga ctgcaggcgc tcccgcttga acagcccgat
     2221 ggctcccgag atgcaggaga tgctggcggt ggcgtcctgg aagcggcggc cgatctccat
     2281 cgacttgcag tactcgtagc gctgcagggc cgcgaggacg ccgctcccct tcggcccgta
     2341 gcggtcacgc cggtcgggga cgacgttgaa ggccacggcg tcgtacttct cgaggagcga
     2401 ggtggggatc ttcgccgccc ccacgcgggt gtcgtcgtcg agcagcagca cgtaaggggt
     2461 cgtgacccgg tggatgccga agttgaccgc cgacaccttc cccatgttcg gggtgcggta
     2521 ggagcggacc ttcatctcgg cgaggagctt cggcgtgtcg tccg
//
LOCUS       DASRIQ010000579         6768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1540268, whole genome shotgun sequence.
ACCESSION   DASRIQ010000579 DASRIQ010000000
VERSION     DASRIQ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540268"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VFD06_14330"
     CDS             complement(<1..988)
                     /locus_tag="VFD06_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04757.1"
                     /translation="MTSRRLALLSVVWLVATGLAHANWTATGTFRYVDREFDQSGFTG
                     AEPALPIRFATVEVRDFNASGNKGLLATGATDANGAFSIAVSDNKVRTVYVRALTSSA
                     AVSGLFFKVQNRFIPKNPYAVASASVPNHNPSTNVNFGTLTAAIGSGGEGFNIYDTGV
                     RVMDYILAVNGSRPGSGSSLTYEWEAGTGAGASSYNPSNRTVTVGDVSGYNDTVFSHE
                     SGHYAYHVYSGSDSPGGSHHLLDCAQDLRLAYEEGRATWFGQSLRRHFSLPNPHLYVK
                     TTGAPGPGNLDFYFDVENETPYACDGATSEVAVYSALWDVNDTAATDDSTPGV"
     gene            complement(985..1326)
                     /locus_tag="VFD06_14335"
     CDS             complement(985..1326)
                     /locus_tag="VFD06_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04758.1"
                     /translation="MASVVLKVSAGVPIDEAVVSARTPSRVVFADGSAARTWKIDLSK
                     GGTVSIPVEVIVPEDGKYSLSVEVSGKAEGKSLRRALSHKLYVGVKERKGKDKDGANE
                     YPAQETEEPRS"
     gene            1724..4177
                     /locus_tag="VFD06_14340"
     CDS             1724..4177
                     /locus_tag="VFD06_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04759.1"
                     /translation="MIDDLRLALRHLLKDRGFTLTAALTLAVCIGANTALFSVVHNVL
                     LSPLPVPESDRLLLMGNSYPKAGADNLGNSGVPDYYDRLRDMNVYEEQAAFTTRTRSI
                     DMSGTPTRVRVMQATPSFFRLVRVPPALGRAFTEDEGEVGHEKKVVLSHGLWQSQFGG
                     DPAAVGKDLRIDGEPHAIVGVMPKDFVFFDPEAVLWTPLAFTNEQKSDDSRHSNNWRN
                     VGRLLPGATLTQAQAQVDAINAANLDRFPQYRTLLINAGFHTQVVGLKDYLVRDIRPV
                     LYLMWAGALFVLLIGGVNIANLVLVRSRARLKELATRLALGAGPLRVARQLVLESLLL
                     TTLSAAAGLFLGYATLRLMGSIDIQDLPRGHEIRINGATVAYVLGLATLIGCALGIIP
                     VAGLRRSNLTIILREEGRTGTSGTGARNLRRGLVVAQVGFAYVLLIGAGLLFASFRQV
                     LSVDPGFRADGVMSASVVLPGTRYKDDASLLAFADEALRKMRALPGVSLAGATDTLPL
                     GGSHSDSVILAEGYQMSPGESVISPNRVVATPGYFEALGATLVAGRFFEERDEAKAPA
                     VAIVDEKLARRFWPGADPIGRRMYLPTDINNLLAVTPETVFITVVGVIRDMKLESLTE
                     GRVGTYYFPLAQSPNDGLTFALKTSGDPAALAGGMRGVLQELDPELPVFEVATLEQVT
                     EKSLVSRRAPVLLSLSFGAVALFLSALGIYGVLAYLVTQRTREIAIRLALGSSAGGIF
                     RLVLREGAWLSAAGFLLGIAGAAALRKGIESQLFGVSPSDPMVLAAVTVGLALVAIAA
                     CALPARRATRIDPILALSE"
     gene            complement(4229..4798)
                     /locus_tag="VFD06_14345"
     CDS             complement(4229..4798)
                     /locus_tag="VFD06_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04760.1"
                     /translation="MHTAMPFGSLWMPVLASAVAVFVASSLLHMFLKYHQADHRRLPA
                     EDAVREGLGRADLAPGVYMTPHCTHQQMKDPAILAKFEKGPVAIITALPKGMPNMGKH
                     LGLWFGLCFLVSFTAGYIARHTLQPGAAGMLVMQITGAVAFAGYSYGQISDTIWKGQP
                     WANTLRHLLDGVIYAVITGIIFLALWPAA"
     gene            4967..5920
                     /locus_tag="VFD06_14350"
     CDS             4967..5920
                     /locus_tag="VFD06_14350"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HZN04761.1"
                     /translation="MPLRFDFNVRNGSRILAPIVFGAIAFRVIRGLAPGAPPSGSLWR
                     EAFLALAGIGLLGYGFVLLHRKRAIDNVPRSRLRSVAMGFAEVSGTARVRTPVTAPYS
                     GIPCVAYRYLAEEEESSSRRGGWRTVERGQSADWFLLDDGTGTIVVDPDGADLHLGRD
                     YRTIERAEGWFGRRRRYTEWRLHPGENACVVGTVRRVRSLVSEQRAALHDRLREVKQD
                     PARLQAFDSDRDGRIDTEEWGNVVRVVTQEVAREAATKEPYRPVDELILGKGEAEKTF
                     LISDRSERDIVRALAWKAGLLLLSGAGMAGAGAYALLQRKP"
     gene            5931..6518
                     /locus_tag="VFD06_14355"
     CDS             5931..6518
                     /locus_tag="VFD06_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZN04762.1"
                     /translation="MMLILLLAFAVTAVVAIGYAVGIYNQLIGVRVNVDRSWSNIEVL
                     EKQRYDEIPKLVKVCEGYMQYERDTLQKVIAARTRYLGARGPANRAAAGAEMAGALKS
                     LFALAESYPDLKANANFTQLQSRITALESEIADRREFYNDSVAIFNARVEQVPYVFFA
                     RPLGYKARDMYKVAPAETKAPDIQFSMPGSGRTAA"
     gene            6541..>6768
                     /locus_tag="VFD06_14360"
     CDS             6541..>6768
                     /locus_tag="VFD06_14360"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HZN04763.1"
                     /translation="MKHIVVCCDGTWNTPDHLDRGRVSPSNVVKTALAVAPVRSDGTP
                     QIVHYDRGVGTGVFDHLRGGAFGFGLSANILE"
BASE COUNT          986 a         2372 c         2331 g         1079 t
ORIGIN      
        1 cgaccccggg ggtggagtcg tcggtcgcgg ccgtgtcgtt gacgtcccac agggccgagt
       61 agaccgccac ctcgctggtc gccccgtcgc acgcgtaggg ggtctcgttc tcgacgtcga
      121 aatagaagtc gaggttgccc gggccggggg ccccggtggt cttgacgtag aggtgcgggt
      181 tggggaggct gaaatggcgt cgcagcgact ggccgaacca ggtcgcgcgc ccttcctcgt
      241 aggccagcct caggtcctgc gcgcagtcga gcaggtggtg cgatcctccc gggctgtcgc
      301 tccccgagta cacgtggtag gcgtagtgcc cgctctcgtg gctgaagacg gtgtcgttgt
      361 agccgctgac gtccccgacc gtcaccgtgc ggttcgacgg gttgtacgat gaagcgccgg
      421 cacccgtgcc ggcctcccat tcgtaggtca gggacgatcc ggagccgggg cgggacccgt
      481 tcaccgccag gatgtagtcc atcacccgca cccccgtgtc gtagatgttg aacccttcgc
      541 cgcccgaccc gatggccgcg gtcagggtgc cgaagttcac gttggtcgac gggttgtggt
      601 tcggcacgct ggccgaggcg acggcgtagg ggttcttcgg aatgaaccgg ttctgcacct
      661 tgaagaacag tccgctcacg gcggcagagc tggtcagggc gcggacgtag acggtccgga
      721 ccttgttgtc cgacaccgcg atgctgaagg cgccgttggc gtcggtcgcc ccggtggcga
      781 gcagcccctt gttgccgctg gcgttgaagt cccgcacctc gacggtcgcg aagcggatcg
      841 gcagcgccgg ctcggcgccg gtgaagccgc tctgatcgaa ctcgcgatcg acgtagcgga
      901 aggtgccggt ggcggtccag ttggcgtggg cgagaccggt cgcgacgagc cagacgaccg
      961 acagcagggc gagacgacgc gaggtcatga gcggggctcc tccgtctcct gcgccggata
     1021 ctcgttcgcc ccgtccttgt ccttcccctt ccgctctttg accccgacgt agagcttatg
     1081 gctcagggcg cgccggagcg atttcccttc cgccttcccc gacacctcga cggagagcga
     1141 gtatttgccg tcctccggca cgatcacctc gaccgggatc gaaaccgtgc cccccttgga
     1201 cagatcgatc ttccaggtcc gggccgcgct cccgtcggcg aagacgacgc gcgacggggt
     1261 tcgggcggag acgaccgcct cgtcgatcgg gacgccggcg ctgaccttga gcacgacgga
     1321 ggccaccccc cctcggacgt tcttctgcag cgcggtggtt tcggcggcga tcccaagagg
     1381 aggctggggc tcggaggtct ctcgcctcgc gtccgaagag ccgggttgtc ccttgtcggc
     1441 ctgctggtgc aggccggctt tcggacgggg cgccgctgcc ccgccgccgg tcccggggac
     1501 cgagcgaatc gcgaggggca gaaggatcag aaggcaggaa aagtgccgtc cacgatgcat
     1561 tccggacctc cctggggctg gcctgtctgt gagacagtcg tagggcgttg ccgcggcgtc
     1621 ggcaatggag ttgcgcgcgt catttacggc ctattgccgc ttccgtcaag aagaatctgc
     1681 cgggaactcc cctcgatcgt tcatcgtatg ctccgggcga gccatgatcg acgacctccg
     1741 actggcactc cggcaccttc tcaaggacag gggcttcacg ctgaccgccg ccctcaccct
     1801 ggcggtctgc atcggcgcca acaccgccct cttctcggtc gttcacaacg tccttctcag
     1861 cccgctgccc gtcccggaat cggatcgcct cctgctcatg gggaactctt atcccaaggc
     1921 gggggcggac aacctgggga attccggcgt gcccgactac tacgaccggc tccgggacat
     1981 gaatgtctac gaggagcagg cggcgttcac cacccgcacc cgcagcatcg acatgagcgg
     2041 caccccgacc cgcgtccggg tgatgcaggc gaccccgtcc ttcttccggc tggtgcgggt
     2101 gccgcccgcc ctcgggcgcg cgttcacgga agatgagggg gaggtcggcc acgagaaaaa
     2161 ggtcgtcctg agccacggtc tctggcagtc gcagttcggc ggcgaccccg cggcggtcgg
     2221 gaaggatctc cggatcgacg gcgagccgca cgcgatcgtc ggcgtgatgc cgaaagactt
     2281 cgtcttcttc gaccccgagg ccgttctctg gacgccgctc gccttcacga acgagcagaa
     2341 gtccgacgac tcccggcaca gcaacaactg gcgcaacgtc ggacgcctcc tgcccggggc
     2401 gacgctgacg caggcgcagg cccaggtgga cgccatcaac gccgccaacc tcgaccgctt
     2461 cccgcagtac cggacgctgc tcatcaacgc cggcttccac acccaggtcg tcggtctcaa
     2521 ggactacctg gtgcgcgaca tccggccggt gctgtacctc atgtgggcgg gggcgttgtt
     2581 cgtgctgctg atcggcgggg tgaacatcgc gaacctggtg ctcgtccgct cgcgcgcccg
     2641 gctcaaggag ctggcgacgc gcctggcgct gggggccggt cccctgcgcg tcgcccgtca
     2701 gctcgtcctc gagagtctcc tcctgacgac cctctcggcg gcggcggggc tgttcctcgg
     2761 atacgccacg ctgcgtctca tggggagcat cgacatccag gatctgccgc gcggccacga
     2821 gatccggatc aatggcgcga ccgtcgccta tgtcctgggc ctcgccaccc tcatcggctg
     2881 cgccctcggg atcatcccgg tggccggcct gcggcgctcc aacctcacca tcatcctgcg
     2941 ggaagaggga aggaccggca cgagcggcac cggggcgcgc aacctgcgcc gcgggctggt
     3001 ggtggcgcag gtcggcttcg cctacgtgct gctgatcggc gcgggactcc tcttcgcaag
     3061 cttccgccag gtcctctccg tcgatcccgg cttccgggcc gacggcgtga tgtccgcctc
     3121 ggtcgtcctg cccggcaccc gctacaagga cgatgcgtcg ctcctcgcct tcgccgacga
     3181 ggcgctccgg aagatgcgcg ccctgccggg cgtgtcgctc gccggcgcga ccgacacgct
     3241 gccgctcggg ggaagccaca gcgacagcgt catccttgcc gaggggtacc agatgagccc
     3301 gggggaatcg gtgatctccc ccaaccgggt ggttgcgacc cccggctact tcgaggcgct
     3361 cggcgcgacc ctcgtcgccg gccgcttctt cgaggagcgg gacgaggcca aggccccggc
     3421 ggtcgccatc gtcgacgaga agctggcgcg gcgcttctgg ccgggggccg acccgatcgg
     3481 gcgacgcatg tacctgccga ccgacatcaa caacctcctc gcggtcaccc ccgagacggt
     3541 cttcatcacc gtggtcggcg tcatccgcga catgaagctg gagagcctga ccgaagggag
     3601 agtcggaacc tactacttcc cgctcgcgca gagccccaac gacggcctga ccttcgcgtt
     3661 gaagacctcc ggagacccgg cggcgctggc gggcgggatg cgcggcgtcc tccaggagct
     3721 cgacccggag ctgccggtgt tcgaggtggc gacgctcgag caggtgaccg agaagtcgct
     3781 cgtgagccgc cgcgccccgg tgctgctgtc cctctccttc ggcgcggtcg ccctgttcct
     3841 gtcggcgctc gggatctacg gcgtgctcgc ctatctcgtg acccagcgga cgcgggagat
     3901 cgccatccgc ctggcgctcg gaagcagcgc cggcggcatc ttccggctgg tgctgcgcga
     3961 aggggcctgg ctgagcgccg ccggcttcct cctggggatc gccggagccg cggcgctgcg
     4021 aaaaggcatc gagagccaac tcttcggcgt cagcccctcc gacccgatgg tcctcgccgc
     4081 ggtcacggtc ggcctcgcgc tcgtggcgat cgccgcctgc gcgcttccgg cgcggcgcgc
     4141 cacccggatc gacccgatcc tcgccctctc ggagtaggcc ccccgctttc ggagcaacgt
     4201 ccggccggtc tgcggccgac ggctttgctt acgcggccgg ccagagcgcc aggaagatga
     4261 tcccggtgat caccgcgtag atgacgccgt ccaggaggtg tcgcagcgtg ttggcccacg
     4321 gctgcccctt ccagatcgtg tcgctgatct gaccgtagct gtagccggcg aacgccactg
     4381 caccggtgat ctgcatcacc agcatccccg ccgcccccgg ctgcagcgtg tggcgggcga
     4441 tgtagccggc ggtgaagctg acgagaaagc acagcccgaa ccacaggccg aggtgtttcc
     4501 ccatattcgg catccccttc gggagcgccg tgatgatcgc caccggccct ttctcgaact
     4561 tcgccagtat cgccgggtcc ttcatctgct ggtgcgtgca gtgcggcgtc atgtagacgc
     4621 cgggcgcgag gtccgcacgc ccgagcccct cgcgcaccgc gtcctccgcc ggaagcctgc
     4681 ggtgatcggc ctggtggtac ttcaggaaca tgtgcaggag cgagctggcg acgaagacag
     4741 cgacggccga ggcgagcacc ggcatccaga ggctgccgaa gggcatcgca gtgtgcatcg
     4801 cagagacctc ctcccacggt ccggcgccgc gggcgccgtc cgggcggccg gaatggtacg
     4861 gcaagtgcgg tcggaggggg gtcgaccccg cggcccggcg ccccgcggcg cggaccgatt
     4921 cccttgcagg tccggccccc gaccctatat tgcccgccgg aggggtgtgc cgttgcgctt
     4981 cgacttcaac gtccggaacg gctcgcggat cctcgcgccg atcgtcttcg gggcgattgc
     5041 gttccgggtc atccgcggcc tcgcccccgg cgcgccgccg agcggttccc tgtggcggga
     5101 ggcgttcctg gcgctggcgg ggatcggact cctgggttac ggcttcgtcc tcctgcaccg
     5161 caagcgcgcc atcgacaacg tgccgcgctc gcgcctccgt tcggtcgcga tgggattcgc
     5221 cgaggtgtcc gggaccgccc gggtccgcac gccggtgacc gccccctaca gcggcatccc
     5281 ctgcgtcgcg taccggtatc tcgccgagga ggaggagtcg tcgagccgac gcggcggctg
     5341 gcgcaccgtc gagcgcggcc agtcggccga ctggttcctg ctcgacgacg ggaccgggac
     5401 gatcgtcgtg gacccggacg gggccgacct gcacctcggc cgcgactacc gcaccatcga
     5461 gcgcgccgag ggatggttcg gccggcgacg ccgctacacc gagtggcgcc tgcacccggg
     5521 cgagaacgcc tgcgtcgtcg gcacggtgcg ccgggtgcgg tcgctggtct ccgagcagcg
     5581 ggcggcgctc cacgaccgcc tgcgcgaggt gaaacaggac ccggcgcggc tccaggcgtt
     5641 cgactccgac cgggacgggc ggatcgacac ggaggaatgg gggaacgtcg tccgcgtcgt
     5701 aacgcaggag gtggcgcgcg aggcggcgac gaaggagccg tatcgccccg tggacgagct
     5761 gatcctcgga aagggggagg cggagaagac ctttctcatc tccgaccgct ccgagcgcga
     5821 catcgtgaga gccctggcat ggaaggccgg gctgctgctc ctctccggcg cggggatggc
     5881 cggggcggga gcgtacgccc tgttgcagcg caaaccatga ggagaccgac atgatgctca
     5941 tcctgctcct ggcgttcgcc gtcacggcgg tcgtggccat cggctacgcc gtcggcatct
     6001 acaaccagct catcggcgtg cgggtgaacg tcgatcgatc gtggtcgaac atcgaggtcc
     6061 tcgagaagca gcgctacgac gagatcccga agctcgtgaa ggtgtgcgag gggtacatgc
     6121 agtacgagcg ggacacgctc cagaaggtga tcgccgcccg cacccggtat ctcggcgcgc
     6181 gaggccccgc caatcgggcc gccgcagggg ccgagatggc gggagccctc aagagcctgt
     6241 tcgcgctcgc cgagtcgtac ccggatctca aggcgaacgc caacttcacc cagctgcagt
     6301 cgcgcatcac cgcgctggag agcgagatcg ccgaccgccg cgagttctac aacgactcgg
     6361 tggccatctt caacgcgcgc gtcgagcagg ttccgtacgt cttcttcgcg cgtcccctcg
     6421 gctacaaggc acgcgacatg tacaaggtcg ccccggcgga gaccaaggcc ccggacatcc
     6481 agttcagcat gccggggtcc ggccggaccg ccgcctgacg cggcctgctc cggcccgatc
     6541 atgaagcaca tcgtcgtctg ctgcgacggg acctggaaca ctcccgatca cctcgaccgg
     6601 ggccgggtca gcccgagcaa cgtcgtcaag acggcgctcg cggtggcgcc cgtgcggtcg
     6661 gacgggacgc cgcagatcgt ccactacgat cgcggggtgg ggaccggggt gttcgatcac
     6721 ctgcggggcg gcgccttcgg cttcgggctg tcggccaaca tcctcgag
//
LOCUS       DASRIQ010000580         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3340724, whole genome shotgun sequence.
ACCESSION   DASRIQ010000580 DASRIQ010000000
VERSION     DASRIQ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3340724"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1796)
                     /locus_tag="VFD06_14365"
     CDS             complement(54..1796)
                     /locus_tag="VFD06_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN04764.1"
                     /translation="MRFGAQVYLWALLLVPVLAGLLALSAWRRRRDLARFGDMLLVRR
                     LAASLSVERRLIKAVLLVMASIFLVLALARPQWGARMETVSRRGIDLVIAVDTSNSML
                     AEDISPNRLAQARGAVLSLLEMLEGDRVGLVAFAGTAYLACPLTLDYGAAGMFVDVLD
                     TDLLPVQGTAIAEAITVATKAFPEGERRYKVIILITDGEDHEGDVEAAATAAADQGIV
                     IHTVGVGGTAGTPIPIRNARGDVTGYKEDQERRKVTSRLNQSALESIAVATRGKYFRA
                     SAEGIELRRVYEEIAAMDRRTLSSRMTSAFEERYIIPLGAAIVLLAIEASIGDRRRTA
                     RAARAAGGSRPGARAAAAALCALLGAMFATPASAADVASRNNEGNQKYEQKKYDEALR
                     LYTDAQALKPSAPELHYNIGNVLFRKGEFDKAIEEYLRAQSAASPGLRQAAVYNQGNA
                     HLQQGKLQDAVNAYVQALRLDPKDQEAKRNLELALRLLEEQKKQQQQQQQQQQPQDKK
                     DQEQKQQEPKPAKPEEPKPDEKKEEQEKRPGQMSEEEARQILQALRENEKEGVRKHAK
                     MQPPSRPRHPEEDW"
     gene            complement(1793..>2137)
                     /locus_tag="VFD06_14370"
     CDS             complement(1793..>2137)
                     /locus_tag="VFD06_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotolerance regulator BatA"
                     /protein_id="HZN04765.1"
                     /translation="LGVKVYTIGVGKEGEAPYPIDDPLMGRRYIMVTADIDEETLKAI
                     AAATDGQFFRATDARSLKAIFERIDRMERTEMKVRGYTRKSERFAMFLYPGALLIGLE
                     LLFGSVVLRRVP"
BASE COUNT          284 a          756 c          728 g          369 t
ORIGIN      
        1 ggccggcgcg gcgagggtcg gcaggaggag gccgagcagg acgacggcgc gtctcaccag
       61 tcctcctcgg gatgacgcgg acgggacggc ggctgcatct tcgcgtgctt cctgacccct
      121 tccttctcgt tctcccggag cgcctgcagg atctgtcgcg cctcctcctc gctcatctgg
      181 ccggggcgct tttcctgctc ctccttcttt tcgtccggct tcggctcctc cggcttggcc
      241 ggcttcggct cctgctgttt ctgctcctgg tctttcttgt cctggggctg ttgttgctgc
      301 tgctgttgct gttgctgctt tttctgctct tcgaggagcc gcagcgcgag ctcgagattg
      361 cgcttcgcct cctggtcctt cgggtcgagc cggagcgcct ggacataggc gttcacggcg
      421 tcctgcagct tcccctgctg caggtgggcg ttgccctggt tgtagaccgc cgcctggcgc
      481 aggccggggc tcgcggccga ctgggcgcgc aggtactcct cgatcgcctt gtcgaattcg
      541 cccttccgga agagaacgtt gccgatgttg tagtgcagct cgggcgccga gggcttgagc
      601 gcctgcgcgt cggtgtacag ccgcagcgcc tcgtcgtact tcttctgctc gtacttctga
      661 ttcccctcgt tgttgcgcga cgcgacgtcg gccgccgacg ccggcgtcgc gaacatcgct
      721 ccgagaagcg cgcacagtgc cgccgcggca gcccgggcgc cggggcgcga cccgcccgcc
      781 gcgcgcgcgg cccgcgccgt gcgacgccgg tcgccgatcg acgcctcgat ggcgagcagc
      841 acgatcgccg ctccgagcgg gatgatgtag cgctcctcga aggccgacgt catgcgcgac
      901 gacagcgtgc ggcgatccat ggcggcgatc tcctcgtaga cgcgccggag ctcgatccct
      961 tccgcggagg cgcgaaagta cttgccgcgg gtcgccaccg cgatcgactc gagcgcgctc
     1021 tggttcaggc ggctggtcac cttgcgccgc tcctggtcct ctttatagcc ggtcacgtcg
     1081 ccacgcgcgt tccggatcgg gatcggcgtg ccggcggtgc cgccgacgcc caccgtgtgg
     1141 atgacgatac cctgatcggc ggcggccgtg gcggccgcct cgacgtcccc ctcgtgatcc
     1201 tccccgtcgg tgatcaggat gatgaccttg tagcgccgct ccccttccgg gaaggccttg
     1261 gtcgccaccg tgatcgcctc ggcgatggcg gtcccctgca ccggcagaag gtcggtgtcg
     1321 aggacgtcga cgaacatccc ggcggcgccg tagtcgagcg tcagggggca ggcgagatag
     1381 gcggtgccgg cgaaggcgac cagcccgacg cggtcccctt cgagcatctc gagaagcgac
     1441 aggacggcgc cgcgcgcctg cgccaggcgg ttcggggaga tgtcctcggc cagcatgctg
     1501 ttcgaggtgt cgaccgcgat gacgaggtcg atgccgcgcc ggctcaccgt ctccatgcgc
     1561 gccccccact gcggccgggc gagcgcgagc acgaggaaaa tcgacgccat gaccaggaga
     1621 accgccttga tgaggcgccg ctcgacgctc aggctcgcgg cgagccggcg gacgagcagc
     1681 atgtccccga aacgcgcgag gtcgcggcgc cggcgccagg ccgacagcgc cagaagcccc
     1741 gccaggaccg gcaccaggag gagcgcccag agatagacct gcgcgccgaa cctcacggca
     1801 ccctcctcag gacgaccgaa ccgaacagga gctcgagccc gatcagaagc gcccccgggt
     1861 agaggaacat cgcgaaccgc tccgacttgc gggtgtagcc gcgcaccttc atctcggtgc
     1921 gctccatccg gtcgatgcgc tcgaagatcg ccttgagcga ccgggcgtcg gtggcgcgaa
     1981 agaactgccc gtcggtcgcg gcggcaatcg ccttgagggt ctcctcgtcg atgtcggcgg
     2041 tcaccatgat gtagcgccgc cccatcagcg ggtcgtcgat cggatagggc gcctccccct
     2101 ccttgccgac gccgatggtg tagaccttga cgccgag
//
LOCUS       DASRIQ010000581         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3340863, whole genome shotgun sequence.
ACCESSION   DASRIQ010000581 DASRIQ010000000
VERSION     DASRIQ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3340863"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFD06_14375"
     CDS             complement(<1..894)
                     /locus_tag="VFD06_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HZN04766.1"
                     /translation="MRVRRGGGDATVRGETAGGRLVLEDGTVFEGRSFGAPVPSAGEV
                     VFTTGMVGYPEALTDPSYRGQILSFTYPSLGNYGVPDYPRGADRSDRATDRAGAADAP
                     AQGPPDVLPGRPWESDRIHASGVVCASYSEEFSHHTARHSLGDWLRASGIAGLTGIDT
                     RALTKRIRTRGSLLGRIEIDGRPAPGFVDPNRRNLVAEVSTPKVRRYGRGTPLVLLDC
                     GRKNNQVRMLVDRGAEVRVVPWDHPLATRPRDYAGVVLSNGPGDPSLVVDAVRTVERL
                     VRQRVPVLGICLGHQILALAAG"
     gene            1225..>2776
                     /gene="asnB"
                     /locus_tag="VFD06_14380"
     CDS             1225..>2776
                     /gene="asnB"
                     /locus_tag="VFD06_14380"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN04767.1"
                     /translation="MDAMSTRGPDDYGIHVDSQVVLGHRRLSILDLSMAGHQPMSRAG
                     GRLWIVYNGEIYNFKSLRRQLEGRGHRFDSRTDTEVILALYEEMGIECLAQLRGMFAF
                     AIWDRREATPVLLLARDHFGIKPLVYATTPRGLEFASDLPGLVAGGGVPRRIDRTALA
                     QYLMHGHVVFPRTMIEGVSMLPPAHSLTIRPGEAPRIRPYWRLDVERCEPLSRRESYG
                     DQTARVRFLLEEAARSQMASDVPLGAFLSGGIDSAGIVALMTRAAGRPIHTFSIGFPQ
                     TGGALDESVDAELTARHLGAVHQAVQVSGTEVAKILPDIAVALGQPTVDGVNMYLVSS
                     AARRDLTVALTGVGGDELFAGYPSFARLARYRHQLFRARQRIGWLKRLPGRRMFPGPG
                     SINRPWTRLEKDRPLVSDYMACRMVRPPAEALRVAGDGTVDPEAFFAYVDSDEPGLAD
                     PVRRVTLLESRLYLAAQILRDTDAASMAHSLEIRVPLLDVDLAEYVYGLPQDAKLAPA
                     STGAAPAGK"
BASE COUNT          388 a         1003 c          980 g          405 t
ORIGIN      
        1 gccggccgcg agggccagaa tctgatggcc caggcagatg ccgagcaccg gcacccgctg
       61 gcgcaccagc cgctcgaccg tccggaccgc gtcgacgacc aggctcggat cgcccggccc
      121 gttgctcagg accaccccgg cgtagtcgcg cgggcgcgtc gcgagcggat ggtcccaggg
      181 aacgacgcgc acctcggccc cgcggtccac cagcatccgc acctggttgt tcttccggcc
      241 gcagtcgagg agcacgagcg gcgtgccccg gccgtagcgg cggaccttcg gagtcgagac
      301 ctcggcgacg aggttgcgcc ggttcggatc gacgaacccc ggcgcgggac gcccgtcgat
      361 ctcgatgcgg ccgaggagcg agccgcgcgt ccggattctc ttcgtgaggg cgcgcgtgtc
      421 gatgccggtc agcccggcga tcccggaagc gcgcagccag tccccgagcg aatgccgcgc
      481 cgtgtggtgc gagaactcct ccgagtagga ggcgcagacg acccccgagg cgtggatgcg
      541 gtccgattcc cacggccggc cgggaaggac gtctgggggg ccctgcgccg gcgcgtcggc
      601 ggcgcccgcg cggtccgtcg cgcggtccga acggtccgcg ccgcgcgggt agtcggggac
      661 gccgtagttc ccgagcgacg gatacgtgaa ggagagaatc tggccgcggt acgaggggtc
      721 ggtgagcgcc tcgggatatc cgaccatccc ggtggtgaag accacctcgc cggcggacgg
      781 aacgggagcg ccgaaggagc ggccctcgaa gacggtgccg tcctccagga ccagtcgtcc
      841 tcccgccgtc tcgccacgca ccgtcgcgtc ccctcccccc cggcggacgc gcacctctcc
      901 tgcaaacggg cggagtgcag gtcagggtcg cgcgtcggcc ggaatcgcgg cgcgaagaat
      961 agcacacgca cgtcgcgctc ccggaaacgg accgcgacct cttttccctt gttcgcctgg
     1021 acgtccgcgg agtgggtgcc ggcgcggagg gagcgggcgg gccaaccctc cggcctggcc
     1081 ggcccctcga ccgcgaggtc ccggatttct cgttgcggcc acggcccggc tgtcgtacct
     1141 tccccggatt aacctgaggg gctgacgatg tgcggaatcg ccgggattat cggtcgcccc
     1201 gatctcgaag ccgtccagtc catgatggac gccatgagca cgcgcgggcc cgacgactac
     1261 ggcatccacg tcgactctca ggtcgtgctg ggccaccgga ggctcagcat cctcgatctc
     1321 tccatggccg gccatcagcc catgtcgcgt gccggcggga gactatggat cgtctacaac
     1381 ggcgagatct acaacttcaa aagcctgcgc cggcagctcg aggggcgcgg ccaccggttc
     1441 gattcgcgca ctgacaccga ggtcatcctc gccctctacg aggagatggg gatcgagtgt
     1501 ctggcacagc tgcgcggcat gttcgcgttc gcgatctggg atcgccgcga ggcgacgccc
     1561 gtgctcctcc tggcgcgcga tcacttcggg atcaagccgc tggtctacgc gacgacgccg
     1621 cgcgggctcg agttcgcctc ggaccttccg ggcctcgtgg cgggcggagg agtgccgcgg
     1681 cgcatcgatc ggacggccct cgcgcagtac ctcatgcacg ggcacgtcgt tttcccgcgc
     1741 accatgatcg agggtgtctc gatgctcccc cccgcgcaca gcctgaccat ccggccgggg
     1801 gaggcgccgc ggatccggcc gtactggcgg ctcgacgtgg agcggtgcga gccgctgagc
     1861 cggcgggagt cctacggcga tcagaccgcg cgggtgcgct tcctgctcga ggaggcggcg
     1921 cggtcgcaga tggccagcga cgtgccgctc ggcgcgttcc tgagcggtgg gatcgactcc
     1981 gcggggatcg tggcgctcat gacgcgcgct gccggccgcc ccatccacac cttctcgatc
     2041 gggttcccgc agaccggggg cgccctggac gagtcggttg acgcggagct gaccgctcgt
     2101 cacctgggcg ccgtccacca ggcggtccag gtctccggga cggaggtggc gaagatcctg
     2161 ccggacatcg cggtggcgct cggacagccg acggtcgacg gcgtcaacat gtaccttgtc
     2221 tcgagcgcgg cccgccgcga cctcaccgtg gccctcacgg gggtcggcgg ggacgagctc
     2281 ttcgcgggct atccgtcctt cgcgcggctg gcccgctatc gccatcagct gtttcgggcc
     2341 cgccagagga tcggctggct gaagcgcctc ccgggacgcc ggatgttccc cggccccggc
     2401 tcgatcaacc gcccgtggac gcgcctggag aaggatcggc ctctcgtctc cgactacatg
     2461 gcctgccgca tggtgcgccc gccggccgag gccctgcgcg tcgccggcga cgggacagtc
     2521 gatcccgagg ccttcttcgc ctacgtcgat tcagacgagc ccgggctggc cgatccggtc
     2581 cggcgcgtga cgctcctcga gagccgtctc tatctcgcgg cgcagatcct gcgcgacacc
     2641 gacgcggcga gcatggcgca ctccctcgag atcagggttc cgctcctcga cgtcgacctg
     2701 gccgaatacg tctatggact gccccaggac gccaagctgg cccccgcctc gacaggcgcc
     2761 gcacccgcgg ggaagc
//
LOCUS       DASRIQ010000582         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3107797, whole genome shotgun sequence.
ACCESSION   DASRIQ010000582 DASRIQ010000000
VERSION     DASRIQ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3107797"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="VFD06_14385"
     CDS             complement(<1..1847)
                     /locus_tag="VFD06_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN04768.1"
                     /translation="MRAAFLLAAAALTSCGDSSAPGAYAPIAGTLQLTIAADGDFGSS
                     FWAVLRRDGADEFRTGPVKAGVRTAIAWKEEVRQPRDWKLKFGPSRHPTYQLIVTRDG
                     GKPDVTLATLAGFNDPAKSLAIAFFAIPLDGEKELKVEIRKPAPIELSVMDSSGAPLE
                     GARILGIATPRYVFEDGFDPEQVDAMHTTWSFDFWNFDHVPNHKIAPDRVKSARTDAK
                     GTCRLEGFLGWVGVSERDERFAMPRSVLAMADVRSARFCVIRTPASVRLTVDGLPGRD
                     TSITERGVVVDGDWPLPEGFKPARWSERLAFLQGKSAEFFTPCRDVRVRPMSAVHRVA
                     SGEAVKGIAPGENRKHQAVVEELEHKTIEGEARFEPSELLSREHCGIELYDQNGRLLR
                     SSGSLGGPGVPKSGRVAFAFHVMDPGPYTLVVRSGNYACVVVRDVKAPREELIVPLSL
                     DGKNVSCSLTVKGSGEGAVVGGWPHRDLVNDFISGSRPAGKPGLNTFVVTGAAGSAVV
                     RDVALTEGKTPSLEVALTPGVKVTGRLVDEEGRPIAGQWVHLSWAGYFRMASAFRWLS
                     DLTGADGRFEIPRVPAGPWRLYLRGSGAPAGGPLTVPAEGAAWDAGDLKV"
     gene            complement(1844..2275)
                     /locus_tag="VFD06_14390"
     CDS             complement(1844..2275)
                     /locus_tag="VFD06_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04769.1"
                     /translation="MATYIGGIVFLLACLLGVPLLKRLLHERAVRRLAMDGVTVVPGF
                     WRPSGLRFQRPGWQGAASFESVGIGGTGSGHLRLKAECTGPERREVGPEMLARLEQLG
                     GRLRATGDGAVEIDGPNFTRPEDLRQFLELCDSIVAGKAAA"
     gene            complement(2285..2866)
                     /locus_tag="VFD06_14395"
     CDS             complement(2285..2866)
                     /locus_tag="VFD06_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04770.1"
                     /translation="MGLALFEFMQMADRIKIFPLIALAVVVVMGLVLYWVATRPRETI
                     TDPVPEAPRPPAAPPKRTVEAPPPTTDPGQKILDGAEGAFTKKMFPTALMFYKDFELR
                     YAGSEVYDRNITLVWERIHTSNAQCPPQQQEADLPAYLDKRRKLADEWKRLKPLMTVP
                     PPAEAKAELEKFMEPLPPMDGRRRIIDAWRESK"
     gene            3017..>3549
                     /locus_tag="VFD06_14400"
     CDS             3017..>3549
                     /locus_tag="VFD06_14400"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN04771.1"
                     /translation="MKLAMNLGRVVFVLLLAAAPSPLGFAQAKKKEAASAVPDWGQFR
                     GPNRDGISPDTGLLKQWPAGGPTLAWKSTGFGAGFSSVVVAGKVLYTLGDTNGAACLI
                     AVNTADGKILWKSPMGDKGGNNGAGPRSTPATDGVLVFALGQEGTLVCVQAATGREVW
                     RMNMRDLGGEMMSGWGYS"
BASE COUNT          539 a         1273 c         1184 g          553 t
ORIGIN      
        1 actttcagat ccccggcatc ccacgccgca ccctccgcgg ggacggtcag cggtccgccg
       61 gccggggctc cggacccccg gagatacaaa cgccacgggc cggcgggcac ccgcggaatc
      121 tcgaatcgac cgtccgcccc cgtcaggtcc gacagccatc ggaacgcgct ggccatccgg
      181 aagtatccgg cccacgaaag atggacccac tgccccgcga tgggcctccc ctcttcgtcg
      241 acgagacgtc ccgtcacctt cacgcccggc gtcagcgcga cctccaggct cggggtcttc
      301 ccttcggtca gggcgacatc cctcacgacc gcgcttcccg cggcgccggt gacgacgaac
      361 gtgttgagcc ccggcttgcc ggccggccgg ctcccgctga tgaagtcgtt caccaggtcc
      421 cggtggggcc agcctccgac gacggcgccc tccccgcttc cttttacggt caacgaacag
      481 gagacgttct tgccgtccag ggaaaggggg acgatcagct cctcgcgcgg cgccttcacg
      541 tcccgcacca ccacgcacgc atagttgccg gaccggacca ccagcgtgta aggacccggg
      601 tccatcacgt ggaaggcgaa cgcgacccgt ccgctcttcg gcacgcccgg gccgcccagc
      661 gagccggagg accgcaggag acgcccgttc tggtcgtaga gctcgatgcc gcagtgctcg
      721 cggctcagga gctccgacgg ctcgaaccgc gcttccccct cgatcgtctt gtgctccagt
      781 tcctcgacca ccgcctggtg cttccggttc tcgccgggag cgatcccctt cacggcctcc
      841 cccgacgcga cccggtgaac cgcgctcatc ggccggacgc ggacgtcgcg gcacggcgtg
      901 aagaactccg cgctcttccc ctggaggaac gcgagccgct cgctccagcg ggcgggcttg
      961 aatccctccg gaaggggcca gtcgccgtcg accacgactc cgcgctcggt gatggacgta
     1021 tcgcggcccg gcaggccgtc gacggtgagc ctcacgctcg cgggcgtccg gatcacgcag
     1081 aatcgggccg accggacgtc ggccatcgcc agcacgctgc gcggcatcgc gaaccgctcg
     1141 tcccgctcgg agactccgac ccagcccagg aagccctcga ggcggcacgt ccccttcgca
     1201 tcggtgcgcg cgctcttcac gcgatcgggc gcgatcttgt ggttcgggac atggtcgaag
     1261 ttccagaagt cgaacgacca cgtggtgtgc atcgcatcga cctgttcggg atcgaagccg
     1321 tcctcgaaca cgtacctcgg cgtcgcgatc cccaggatcc gcgcgccctc cagcggcgcg
     1381 cccgaggaat ccatcacgga cagctcgatc ggagcgggct tccggatctc gaccttgagc
     1441 tccttctctc cgtcgagcgg aatggcgaag aaggcgatcg cgagcgactt cgccgggtcg
     1501 ttgaagccgg cgagcgtggc gagcgtgacg tccggcttcc ccccgtcgcg cgtcacgatc
     1561 agctggtagg tggggtggcg ggacggaccg aacttcagct tccagtcgcg cggctggcgg
     1621 acctcctcct tccacgcgat cgccgtgcgg acgcccgcct tcaccggccc ggtcctgaac
     1681 tcgtcggcgc cgtcgcgccg gaggaccgcc cagaaggacg agccgaagtc gccgtcggcg
     1741 gcgatcgtca gctgcagcgt tccggcgatc ggcgcgtacg cccccggggc cgacgagtcc
     1801 ccgcacgacg tcagcgccgc cgcggcgagc aggaaggccg ccctcatgcg gccgcctttc
     1861 cggcgacgat ggaatcgcac agctccagga actgccggag atcctcgggc cgggtgaagt
     1921 tcggaccgtc gatctcgacg gcgccgtcgc cggtcgcgcg cagccggccg ccgagctgct
     1981 ccagccgggc gagcatttcc ggtccgacct cgcgccgctc cgggccggtg cattcggcct
     2041 tgagccgaag atggccggaa ccggtgcctc cgatgcccac ggactcgaac gacgcggctc
     2101 cctgccaccc cgggcgctgg aaccggaggc cgctcggcct ccagaagccc gggacgacgg
     2161 tgacgccgtc catcgccagg cgcctcaccg cgcgctcatg aaggagccgc ttcaggagcg
     2221 ggacgccgag aaggcacgcc agcaggaaga cgatgccgcc gatgtaggtg gccatgtcgg
     2281 ggcgttactt cgattcccgc caggcgtcga tgatcctccg ccggccgtcc atgggaggaa
     2341 ggggctccat gaacttctcg agctccgcct tcgcctccgc cgggggcggg accgtcatga
     2401 gcggcttgag ccgcttccat tcgtcggcga gcttccggcg cttgtcgaga taggcgggaa
     2461 ggtccgcctc ctgctgctgc ggcgggcact gggcgttgct ggtgtggatc cgctcccaga
     2521 cgagcgtgat attgcggtcg tagacctcgc tcccggcgta gcggagctcg aagtccttgt
     2581 agaacatcag cgccgtgggg aacattttct ttgtgaacgc gccttcggcg ccgtcgagga
     2641 tcttctgtcc gggatcggtc gtgggcggcg gggcctcgac cgtcctcttg ggcggcgcgg
     2701 cgggcgggcg cggggcctcg gggacggggt ccgttatcgt ctcgcgggga cgggtcgcga
     2761 cccaatagag cacgagcccc atgaccacga cgaccgcgag ggcgatgagg gggaagatct
     2821 tgatccggtc tgccatctgc atgaactcga agagggccaa tcccatcgtt atagcaaatc
     2881 ccggtcccgg gcttgtaaca accgggaatt ccacccgatt ccggatatcc tcacccctcc
     2941 ccgtccgttt ctccccgggc tggcgtgtat aacttattag accgataatg gatccctgtc
     3001 tttggagact ttcaccgtga agcttgccat gaacctgggg cgggtcgtgt tcgtcctcct
     3061 gttggcggcg gcgccgagcc cgctcggctt cgcccaggcc aagaagaagg aagcggcgtc
     3121 cgccgttccc gactggggcc agttcagggg tccgaaccgg gacggcatct cccccgacac
     3181 cgggctgctc aagcagtggc ccgccggagg accgaccctc gcgtggaagt ccaccgggtt
     3241 cggcgccggg ttctcgagcg tcgtcgtcgc gggcaaggtc ctctacacgc tcggcgacac
     3301 gaacggcgcg gcctgcctga tcgcggtcaa cacggccgac ggcaagatcc tgtggaagag
     3361 cccgatgggc gacaaggggg gcaacaacgg cgccggtccc cggagcacgc ccgccaccga
     3421 cggcgtcctc gtcttcgccc tcggccagga aggcacgctc gtctgtgtcc aggcggcgac
     3481 gggccgcgag gtctggcgca tgaacatgag ggacctcggc ggcgagatga tgtccggctg
     3541 gggctattc
//
LOCUS       DASRIQ010000583        11586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2306807, whole genome shotgun sequence.
ACCESSION   DASRIQ010000583 DASRIQ010000000
VERSION     DASRIQ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2306807"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VFD06_14405"
     CDS             complement(<1..1472)
                     /locus_tag="VFD06_14405"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HZN04772.1"
                     /translation="MSGAGAILIGTLGAGAAVAQEGPPAPMLLVGYSGLEWTVSEGAT
                     GYDVIRGDVRLLRDAADGFTSAVRVCLADDQVASQLSYQGIPKPGEAFFFLVRANAGD
                     TVGTFDSGGTGQSAPRDAAIDAATKSCFSSVTPHPPIVIGGDNQFTAQRGVVAGRGTL
                     GDPYLLAGWDIECSRPTNTTGIEIRHTTLAYTIRNVRVRSCGTGILLDRTWNGRVERS
                     RLAANREGVRVSESPVALDGNVIDGIDEAAIRLEAGSSALVQRNTVSGSGIGIDLDGA
                     LSSTIRGNTLLSNATHALDRNGASNAWNSQYPQDWSNPFFPQGGNYWSGYAGGDSCSG
                     AQQSVCSGGRPDGFGDTPVTFALAGIDAYPLMRPASDEGDTLPPTLQIDAPALSFIPT
                     ITITGAVQDAGTGVLGVIVRTNYGPWMSKGGGATLSFTATLVPGENRIEAQAQDRAGN
                     LSPIAGVIVTYQPPTPVVWQAVLETSQASYPPGTAVTFTVR"
     gene            1550..2410
                     /gene="hemQ"
                     /locus_tag="VFD06_14410"
     CDS             1550..2410
                     /gene="hemQ"
                     /locus_tag="VFD06_14410"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HZN04773.1"
                     /translation="MRYTDSMRHPNVPETLEGWSVLHQMFRVRWEAWRALPAERRKAL
                     AREASELLAGMEADAEGPSALVSLLGHKGDLMVIHFRRDFERLQAAELAVAQTGLHAH
                     LEPTTSYVSIVELGLYDMTAKIHEEMHGRGLTRGTPEFDRAFDAEMQKQRERMMGRLY
                     GPVPAGRYACFYPMDKRRGETANWYTVPFEKRAAMMREHGFIGREYTGQVTQIVSGSI
                     GLDDWEWGVDLFADDPLVFKKLIYDMRFDEASALYALFGPFYVGLRFPARDLPQLLDG
                     RVPGPPAAGS"
     gene            2494..3264
                     /locus_tag="VFD06_14415"
     CDS             2494..3264
                     /locus_tag="VFD06_14415"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HZN04774.1"
                     /translation="MMDWLNALFRWIHVVAGIAWIGHLYFFNFVNAQVAKTYDADSKK
                     KVVPELMPRALYFFRWGAAWTWITGILLFGLVYPMGGALLDTDPTMSSGVASVIALVV
                     LFAAFFVYDLLWKSPLAKNAMAANAVSIVLIAVLAFFLARTFSGRASYILLGATLGTI
                     MAANVWMRIWPSQRRIIGAIKNGQAPDAAIVALAGLRSRHNTFMSVPLVFLMVSNHYP
                     TLYGSPYAWAWALVIVGLGFALVSWLYAKSATPAPAQF"
     gene            3273..4115
                     /locus_tag="VFD06_14420"
     CDS             3273..4115
                     /locus_tag="VFD06_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZN04775.1"
                     /translation="MARIPDGIGLRGAAAALGLIGAVFAASCGGGGGGSESSKGGGAE
                     APAPAAPAPSAPGAIPAGQSIGSASVTGRALFRGKAPKRRPIAMSADPGCKHPGGGET
                     LSDDVLISADGALQNVWVRVVSGLGDKTFAPPAEPVALDQTGCSFTPHAATAQTGQTI
                     LFKNGDSVAHNVHALPKANPGFNVTLPPSGKPSERRFTKPEAVRVKCDIHPWMGSWVL
                     VHDAPFQALTGEDGRFAIRGLPAGTYEIEAWHERLGAAARQSVTLGDGEEKAIDFSFA
                     APST"
     gene            complement(4182..4442)
                     /locus_tag="VFD06_14425"
     CDS             complement(4182..4442)
                     /locus_tag="VFD06_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04776.1"
                     /translation="MRDIELGLRMLHTGLRRLARDVTSMRMSGNGRGAARRRPVTRAL
                     RLQGRYMGLIRTLPLRLKVQVKAIRAKQGVEQAIRKARALKD"
     gene            complement(4519..5724)
                     /locus_tag="VFD06_14430"
     CDS             complement(4519..5724)
                     /locus_tag="VFD06_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HZN04777.1"
                     /translation="MPIGTAVHDRTLALCESLNYREWSGYFAVSSYEVHFHHEYNAIR
                     NSAALIDISPLFKYRVSGPDARRLVDRIVTRDMTKVAVGQMVYTPWCDERGKVVDDGT
                     VTRLEEDVYRMTAADPTLRWIRDNSAGLNVKVEDVSNAVAALALQGPTSAAVLRSAEQ
                     ADIPGLRYFRMTRGTIDGVPVEITRNGYTGDLGYEIWIPWESALRVWDALMRAGRAHG
                     IRAAGMLALDVSRIEAGLLLIEVDYVSSRRATIDDQTSTPDELGWDRLVHLDKQEPFV
                     GRAALRAERAKGAPVRRIVGLDLDWTDVERLHDRFHLTPSIPAAASRVGVPVFRGDRQ
                     IGKATSTTWSPTLKKMIALATVESSHAAPGTTLQMEITVEAHRLKAGARVTPLPFFNP
                     SRKTAVPAA"
     gene            6030..7625
                     /locus_tag="VFD06_14435"
     CDS             6030..7625
                     /locus_tag="VFD06_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009470192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN04778.1"
                     /translation="MAQRYDAIVIGGGHNGLVHAAYLAKAGKKVVVLERRHVLGGAAV
                     TEEVFPGFKFSVFSYVVSLLRPEIIRDLDLPRHGLEILPLDGTFTPLDGDYLWRMDDH
                     GRSIREIARHSRTDAETYEEFGRAMQALCRFVRPILSMLPPDPAKPDVKDLLNLLFLG
                     RRLKSLPYEDRYNLVQLMTMSAADFLDQWFETPALKGTMSASGIIGTFLGVRSPGTAY
                     VLLHHYMGEIDGAFRAWGFAKGGTGGISNAIGSSARELGVEIRREAPVARIIVKHGKV
                     KGVALESGDEIHADLVSSSLDPRLTFLKLLEPGAVSDDFLEEVRHYKFRGSSGKVNLA
                     LDGLPDFTCLPGPGAHLRGAISISPSMDYMEKAYDEAKYGAFSKRPYIDIVIPSLTDP
                     SVAPPGKHVMSCFVQYAPYNLKEGPQDWDNQREAFGDTVVNTIAERAPNLKKLILHRQ
                     VMTPLDIERITGLSEGNIFQGELSLEQLFFLRPVAGWARFRTPVRNLYMCGSATHPGG
                     GIMGAPGRLSALEVLKDWKRGIA"
     gene            7622..9223
                     /locus_tag="VFD06_14440"
     CDS             7622..9223
                     /locus_tag="VFD06_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN04779.1"
                     /translation="MSARQDVVILGGGLNSLVAAFDLARAGRRPVLLERRPVLGGIAV
                     TEEIAPGFKAPRLMHLAGPLRPDLLADLNLTHHGLKWLESPVLMAAASPEGRPLVLHR
                     DPKRSAESIAAFSKKDAERWPKFEEAVGRIAGAMGGLLDLIPPDIDKPAWKEVFPLLK
                     TGKKVRDLGGRDLYRVLRWMPMAVADLAAEWFESEIVRAAIASRAILGQAAGPWSAGT
                     AANYFLRAAADPRAIPSVVVPEGGIGALTQAIAAAAKAAGATLRTGVEVTGIEVKNGA
                     VEAVRMTQGDRIEATSIVSGLDPKRTLLGLVAPADLDPAFRKKVQNIRARGVTAKVNL
                     ALGGLPDFKAFGSQAGAGTDGGSPLRGRILFGTSIDDLERAFDASKYGEISEHPYLEA
                     TLPTLLDPSLAPPGKHVLSVLVQYAPHALREGDWNARREELGERVVKTLAAAAPNLPD
                     LIERGQVITPKDLEDDWGLTGGHLFHGEESLDQIFTMRPVLGWARYRTPVRGLYLCGS
                     GTHPGGGLTGANGRNAARALLADRR"
     gene            complement(9278..10648)
                     /locus_tag="VFD06_14445"
     CDS             complement(9278..10648)
                     /locus_tag="VFD06_14445"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZN04780.1"
                     /translation="MATLTAKEARALADRILSFSRVEECEVTIEARESAHTRFAASDV
                     TTAGIATTLALTITSRKRGKSGTVQVSETDPASLERAVRRSEEILGLAPESPEWVEEL
                     GPQTYPAIEAFDAATAAAGPEMRRDGAAAAIEAARGASLAASGFFSTDITSSAIANRK
                     GLFGFQRATEASFSTTMRTPDGTGSGWAGRTATRLADLKTGEIAATAAKKALASAKPK
                     DLEPGRYTVLLEPQAVADLLGTMIGGLQARAADEGRSVFSKPGGGNRVGEKVFAEMVT
                     LRSDPFDARVPGRPWIVPLGFGGGGGGAFGAGAQNQGLPAARVTWVEKGTLRNLSVDR
                     YWARKTNAPPVPYPGSLILEGGSGTLDDLVASCTRGLIVTRFWYIRTLNPQTLQQTGL
                     TRDGVWLVEKGKIVAPVNNFRFNDGPATLLRNVEAMSASVRAGRMVVPAVRARDFNMA
                     SRSDAV"
     gene            complement(10662..>11586)
                     /locus_tag="VFD06_14450"
     CDS             complement(10662..>11586)
                     /locus_tag="VFD06_14450"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZN04781.1"
                     /translation="ELTATAVEKGRKTKSRTYRPHAVAAGWEAVERARYADHARRIAE
                     EAVAHLKAPSVDPGTKDLVLMPSHLGLTIHESLGHSTELDRALGYEADLAGTTFLTPD
                     KLGKFRVGSDLVNVNGDRVRPESLSTCGWDDDGVKTRQFPLFKKGIFSGYQTIRDQAH
                     LIGQEESTGCCYADSYASVPFQRMPNVWLEPAQEPVSLDDLVSGVDEGILIDGRGSWS
                     IDQQRYNFQFGGDVFWEIKGGKVGRMLADVAYQSRTPDFWQACDGLGGDATWENVGLQ
                     NDGKGQPQQVNAMSHGCPPARFRRVNVLRTE"
BASE COUNT         1710 a         4143 c         3938 g         1795 t
ORIGIN      
        1 cgcaccgtga aggtcacggc cgttcccggc ggatacgacg cttggctcgt ctccagaacc
       61 gcctgccaca ccacgggagt gggaggctgg taggtgacga tgacccccgc gatgggagag
      121 agattgccgg cccgatcctg cgcctgagcc tcgatccggt tctcccccgg cacgagggtc
      181 gcggtgaagg acagcgtcgc gccgcctccc ttgctcatcc atggtccgta gttggtccgg
      241 acgatgaccc caagcacgcc cgtcccggca tcctgcactg caccggtgat cgtgatggtg
      301 gggatgaagc tcaaggccgg cgcgtcgatc tggagggtcg gcgggagggt atccccctca
      361 tcgctggccg gcctcatcag gggatacgcg tcgattcccg cgagggcgaa cgtcacgggc
      421 gtgtcgccga acccatctgg ccggccgccg gagcagacgc tctgctgcgc tccggagcag
      481 gagtcacccc cggcatagcc tgaccagtag ttgcctccct gcggaaagaa cgggttcgac
      541 cagtcctggg ggtactggga attccacgcg ttcgaggcgc cgttgcggtc gagggcatgg
      601 gtggcgttgc tgagaagcgt gttgcctcgg atcgtggaag agagcgcccc atcgaggtcg
      661 atgccgatgc ccgagccgct cacggtgttg cgctgcacca gcgccgacga ccccgcctcg
      721 agacggatcg ccgcctcgtc gatcccgtcg atcacattgc catcgagcgc cacgggactc
      781 tccgagacgc gcacgccttc gcggttcgcc gcgaggcggg aacgctccac ccggccgttc
      841 cacgtcctgt cgagcaggat gccggtgcca cacgaacgga cgcggacgtt ccggatcgtg
      901 taggcgagcg tggtatggcg gatctcgatg ccggtcgtgt tggtcggcct gctgcactcg
      961 atgtcccagc ctgcgaggag ataggggtct cccagggtgc cgcgcccggc gaccacaccg
     1021 cgctgagcgg tgaactggtt gtccccgcca atcacaatcg gcgggtgcgg cgtcacggac
     1081 gagaagcacg acttcgtggc ggcatcgatc gccgcatcgc gtggcgccga ctgccccgtg
     1141 ccccccgaat cgaacgtccc gacggtgtcc ccggcgttcg cgcgaacgag gaaaaagaac
     1201 gcctcgcccg gcttggggat cccctggtaa gagagctggc tggccacctg gtcatccgcc
     1261 aggcagacgc ggaccgccga cgtgaagcca tcggccgcat cccggaggag ccggacatcg
     1321 ccccggatga cgtcgtatcc ggtcgcgccc tccgacacgg tccactccag cccggaatag
     1381 ccgaccagga gcatgggcgc cggggggcct tcctgcgcca ccgcggcgcc ggcgccgagc
     1441 gtcccgatca ggattgctcc agcgcccgac aagagtcctc gaaggattcc catggggtcc
     1501 acctcactgc tcccgaccgg ttagtcgggc ccatctgtct ccaggttcca tgcgctacac
     1561 tgactccatg cgacatccca acgtccccga aacgctcgaa ggctggtcgg tgctccacca
     1621 gatgttccgc gtccgctggg aggcgtggcg ggcgctgccg gccgagcgga ggaaggcgct
     1681 ggcgcgggag gccagcgagc tgctcgccgg gatggaggcc gacgccgagg ggccgagcgc
     1741 gctcgtgagc ctgctcggcc acaagggtga tctcatggtc atccacttcc ggcgcgactt
     1801 cgagcggctg caggcggccg agctggcggt ggcgcagacg ggcctccatg cccacctcga
     1861 gccgaccacc tcctacgtgt cgatcgtcga gctcgggctc tacgacatga cggcgaagat
     1921 ccacgaggag atgcacggcc ggggcctgac gcgcggcacg ccggagttcg accgcgcgtt
     1981 cgacgcggag atgcagaagc agcgcgagcg gatgatgggg cgcctgtacg ggccggtccc
     2041 cgcggggcgg tacgcctgct tctatccgat ggacaagcgc cgcggcgaga cggccaactg
     2101 gtacacggtg ccgttcgaga agcgggccgc gatgatgcgc gagcacgggt tcatcggccg
     2161 cgagtacacc ggccaggtga cgcagatcgt cagcggctcg atcggcctcg acgactggga
     2221 gtggggggtg gatctgttcg cggacgaccc gctcgtcttc aagaagctga tctacgacat
     2281 gcggttcgac gaggcgagcg cgctgtacgc cctcttcggc ccgttctacg tcgggctgcg
     2341 cttccccgcg cgcgatctcc cgcagctcct cgacggccgg gtcccggggc cccccgccgc
     2401 cggttcttga cagccgtccg gcggaccgct agcctccctc gtccgtccac gcgtttccat
     2461 tcacctccga cggggtgagg agggtcccgc agcatgatgg actggctgaa cgctctcttc
     2521 cgctggatcc acgtggtcgc cggcatcgcc tggatcggcc acctgtactt tttcaacttc
     2581 gtcaacgcgc aggtcgcgaa gacctacgac gccgactcga agaagaaggt cgttcccgag
     2641 ctgatgcctc gcgccctgta cttcttccgc tggggggccg cctggacctg gatcaccggg
     2701 atcctgctgt tcgggctcgt gtatccgatg ggcggggccc tgctcgatac cgacccgacc
     2761 atgagcagtg gtgtcgcgtc cgtcatcgcg ctggtcgtgc tcttcgccgc gttcttcgtc
     2821 tacgacctgc tgtggaaatc gccgctggcg aagaacgcca tggccgccaa cgcggtgtcg
     2881 atcgtcctca tcgccgtcct ggcgttcttc ctcgcccgga ccttctccgg gcgcgcctcg
     2941 tacatcctcc tcggggcgac gctcggcacg atcatggccg ccaacgtctg gatgcggatc
     3001 tggccgtcgc agcgccggat catcggcgcg atcaagaacg ggcaggcgcc ggacgccgcc
     3061 atcgtggcgc tcgccgggct gcgctcccgg cacaacacct tcatgtcggt gccgctcgtc
     3121 ttcctgatgg tgagcaacca ctatccgacg ctgtacgggt ccccgtacgc gtgggcgtgg
     3181 gcgctcgtga tcgtcggcct cgggttcgcc ctcgtctcct ggctctacgc gaagtcggcg
     3241 acccccgcac cggcgcagtt ctgaggacgg cgatggcccg cattcccgac ggcatcggcc
     3301 tgcgcggcgc ggccgcggcg ctcggcctca tcggcgccgt cttcgccgcg tcctgcggcg
     3361 ggggcggtgg tggaagcgaa tcctccaagg ggggcggcgc ggaggcaccg gctccggcgg
     3421 cccccgctcc gtccgcgccg ggggcgatcc cggccgggca gtcgatcggc tccgcctccg
     3481 tcaccggtcg tgcactcttc agggggaagg cgccgaagcg gcgcccgatc gcgatgtcgg
     3541 ccgatcccgg ctgcaagcat cccgggggcg gcgagacgct gagcgacgac gtgctgatct
     3601 cggccgacgg cgcgcttcag aacgtctggg tccgggtcgt ctccgggctc ggcgacaaga
     3661 ccttcgctcc gccggcggag ccggtcgcgc tcgaccagac gggctgcagt ttcacgccgc
     3721 acgcggcgac ggcccagacc ggtcagacga tcctcttcaa gaacggcgac agcgtcgccc
     3781 acaacgtgca cgcgctcccg aaggcgaacc cgggattcaa cgtgacgctg cctccgtccg
     3841 ggaagccctc ggagcgccgc ttcacgaaac cggaggcggt gcgcgtcaag tgcgacatcc
     3901 atccctggat gggatcctgg gtgctggtgc acgacgcgcc gttccaggcg ctgaccgggg
     3961 aagacggccg cttcgcgatc cgcggcctgc cggcggggac ctacgagatc gaggcgtggc
     4021 acgagcgcct cggcgctgcc gccaggcaat cggtcacgct cggggacggc gaggaaaagg
     4081 ccatcgactt ctcgttcgcg gcgccgtcga cgtagcgcgg ccgttcgcga acgagtccgg
     4141 gcgcccgccg ggcggcggga cgccctcgag gatcggcgcg atcagtcctt cagcgcgcgg
     4201 gccttgcgga tcgcctgctc cacgccctgc ttggcccgga tcgccttcac ctggaccttg
     4261 agccgcagcg ggagcgtgcg gatcaggccc atgtagcgcc cctgcagcct cagcgcgcgc
     4321 gtcaccggac gccggcgcgc ggcaccgcgt ccgttcccgg acatccgcat cgaggtgacg
     4381 tcgcgcgcca gcctccgcag tccggtgtgg agcatcctca gtcccaactc gatatcgcgc
     4441 atggcccggg ctcgccgggc gctggatttc cgtggcatca acgacctccg tttcgaacgt
     4501 cgtgaaaaaa ggaccgtctc aagccgcggg gaccgcggtc ttgcgcgacg gattgaagaa
     4561 cggcaacggc gtcacccgcg cccccgcctt caggcggtgc gcctcgacag tgatctccat
     4621 ctgcagcgtg gtgccgggcg cggcgtgcga cgactcgacc gtcgccagcg cgatcatctt
     4681 cttgagcgtc ggagaccagg tggtggaagt ggccttcccg atctgccggt cgccgcggaa
     4741 aaccggcaca ccgacgcgcg aggcggcggc cgggatcgac ggcgtcaggt ggaagcggtc
     4801 gtgcagccgc tcgacatcgg tccagtcgag atcgagcccg acgatccggc gcaccggggc
     4861 gcctttggcc cgctcggccc ggagcgccgc acgcccgacg aacggctcct gcttgtcgag
     4921 gtgcacgagg cggtcccacc ccagttcgtc cggagtcgaa gtctggtcat cgatggtggc
     4981 gcgtcgcgac gagacgtagt ccacctcgat gagcagcagc cccgcctcga tccgcgacac
     5041 gtcgagcgcc agcatcccgg cggcgcggat accgtgcgcc cggccggcgc gcatcagcgc
     5101 gtcccagacg cggagggcgc tctcccacgg gatccagatc tcgtagccga gatcgccggt
     5161 gtagccgttc cgggtgatct ccaccggcac gccgtcgatc gtcccgcgcg tcatccggaa
     5221 gtagcggaga ccggggatgt cggcctgctc cgcgctgcgc agcaccgccg cggaggtcgg
     5281 cccctggagc gccagcgccg ccacggcatt ggagacgtcc tcgaccttga cattgagccc
     5341 cgccgaattg tcgcggatcc agcgcagggt cgggtcggcg gcggtcatcc ggtacacgtc
     5401 ctcctcgagc cgcgtcaccg tgccgtcgtc caccaccttg ccgcgctcgt cgcaccacgg
     5461 cgtgtagacc atctgcccca ccgcgacctt cgtcatgtcc cgcgtcacga tccgatccac
     5521 cagacgccgc gcgtcgggcc cgctcacgcg gtacttgaag aggggcgaga tgtcgatgag
     5581 ggcggcggaa ttgcggatgg cgttgtactc gtggtgaaag tggacctcgt aggagctcac
     5641 cgcgaaatac cccgaccatt cgcggtaatt gagactttcg cagagcgcca gagtcctgtc
     5701 gtgaaccgcg gttccaatcg gcacgccgtc cccctgtcct gaggaaggcg cgcattatag
     5761 cgatcgccat cccggggatc aaatcgagac ccgattggat caatcagtcg tgatggcccg
     5821 cctgccggca agccgatcgc ggcgcctgtg gtcaccgggc ggtccggcgt cgagcgctgg
     5881 ccacccacgg cccgcctccc gcccctgttg cgtcgcccgg cgcgcttccc tagaatgtgc
     5941 gctctacacg caacgccgtt tcgcgatgcg caccagccct gtggagggtc ccggggcggc
     6001 gcctcgcgcg gttccgggag aagtcaggca tggcgcagcg ctacgacgcg atcgtcatcg
     6061 gcggggggca caacggcctc gtgcacgccg cctacctcgc gaaggcgggg aagaaggtcg
     6121 tcgtgctcga gcgccggcac gtgctcggcg gcgcggcggt caccgaggag gtcttccccg
     6181 gcttcaagtt ctcggtcttc tcgtacgtcg tgtcgctcct gcgccccgag atcatccgcg
     6241 atctcgatct gccgcgccac ggcctcgaga tcctgccgct cgatggcacc ttcaccccgc
     6301 tcgacggcga ctacctctgg cgcatggacg accacggccg gtcgatccgc gagatcgcgc
     6361 gccactcccg caccgacgcg gagacgtacg aggagttcgg ccgggccatg caggcgctct
     6421 gccgtttcgt gcgcccgatc ctcagcatgc tgccgccgga cccggcgaaa ccggacgtca
     6481 aggacctgct caatctcctc ttcctcggcc ggcgcctcaa gagcctgccg tacgaggatc
     6541 gctacaacct ggttcagctc atgacgatga gcgccgccga cttcctcgac caatggttcg
     6601 agacaccggc gctcaagggg acgatgtcgg cctccgggat catcggcacc tttctcgggg
     6661 tgcggtcccc cggcaccgcc tacgtgctgc tgcaccacta catgggggag atcgacggcg
     6721 cgttccgcgc ctggggcttc gccaagggcg ggaccggcgg gatcagcaac gccatcggca
     6781 gcagcgcccg cgagctcggc gtcgagatcc gccgggaagc gccggtggcg cgcatcatcg
     6841 tcaagcacgg caaggtgaaa ggcgtcgcgc tcgagtccgg cgacgagatc catgccgacc
     6901 tcgtctcctc gagcctcgac ccgcgcctga ccttcctgaa gctcctcgag ccgggggcgg
     6961 tctccgacga ttttctcgag gaggtgcggc actacaagtt ccgcggatcc tccggcaagg
     7021 tgaacctcgc gctcgacggg ctccccgact tcacctgcct gccgggcccc ggggcgcacc
     7081 tgcgcggagc catctccatc tctccctcga tggactacat ggaaaaggcg tacgacgagg
     7141 cgaagtacgg cgccttctcg aagcgcccct acatcgacat cgtcatcccg agcctcaccg
     7201 acccgtcggt cgctccgccg ggcaagcacg tcatgtcctg cttcgtgcaa tacgcgccct
     7261 acaacctcaa ggaggggccg caggactggg acaaccagcg cgaggcgttc ggcgacaccg
     7321 tcgtcaacac catcgccgag cgggcgccga acctcaagaa gctcatcctg caccgccagg
     7381 tcatgacgcc gctcgacatc gagcgcatca ccggcctctc ggaagggaac atcttccagg
     7441 gggagctgtc gctcgagcag ctgttcttcc tgcgcccggt ggccggctgg gcccgcttcc
     7501 gcacccctgt ccgcaacctc tacatgtgcg gctccgccac gcatccgggc ggcggcatca
     7561 tgggggcgcc gggacgactc tcggcgctcg aggtcctgaa ggactggaag cgggggatcg
     7621 cgtgagcgcc cgccaggacg tcgtcatcct cggcggcggg ttgaactccc tcgtcgccgc
     7681 tttcgatttg gcgcgggccg ggcggcgccc ggtgctcctg gagcggcggc cggtcctcgg
     7741 agggatcgcg gtcaccgagg agatcgcccc cggcttcaag gcgccgcgcc tcatgcacct
     7801 cgcggggccg ctgcgacccg acctgctcgc cgatctcaat ctgacgcacc acgggctcaa
     7861 gtggctcgaa tcccccgtcc tgatggccgc ggcgtcgccg gagggacgcc ccctcgtgct
     7921 gcaccgcgac ccgaagcgct ccgccgagtc gatcgccgcc ttctcgaaga aggacgccga
     7981 gcgctggccg aagttcgagg aggcggtcgg ccgcatcgcg ggggcgatgg gcgggctcct
     8041 ggatctcatc ccgcccgaca tcgacaagcc ggcctggaag gaggtcttcc cgctcctcaa
     8101 gaccggcaag aaggtgcgcg acctcggcgg cagggatctc taccgggtgc tgcgctggat
     8161 gccgatggcg gtcgccgatc tcgccgcgga atggttcgag agcgagatcg tccgggccgc
     8221 gatcgcgtcg cgcgccattc tcggccaggc ggccggcccg tggtccgccg ggaccgcggc
     8281 gaactacttc ctgcgcgccg ccgccgatcc acgcgccatc ccctcggtcg tcgtgccgga
     8341 ggggggaatc ggggcgctca cccaggcgat cgccgcggcg gcgaaggcgg ccggcgcgac
     8401 gctccggacc ggcgtcgagg tcaccgggat cgaggtgaag aacggcgccg tggaggcggt
     8461 ccggatgacc cagggggaca ggatcgaggc gacctccatc gtgtcggggc tcgatccgaa
     8521 gcgcaccctg ctgggactgg tggctccggc cgacctggac ccggcgttcc gcaagaaggt
     8581 gcagaacatc cgggcgcgcg gcgtgacggc gaaggtgaac ctggcgctcg gcggactgcc
     8641 tgacttcaag gcgttcggat cgcaggccgg cgccggcacc gacggcggct cgccgctgcg
     8701 cggccggatc ctgttcggga cgagcatcga cgacctcgag cgggccttcg acgcgtcgaa
     8761 gtacggggag atctcggagc atccgtatct cgaggcgacc ctcccgaccc tgctcgaccc
     8821 gtccctcgcc ccgcccggaa agcacgtcct atcggtgctg gtgcagtacg cgccgcacgc
     8881 tctgcgcgag ggggactgga acgcgcgccg cgaagaactg ggggagcgcg tcgtgaagac
     8941 gctcgcggcg gcggcgccga accttcccga cctcatcgag cgcggccagg tgatcacgcc
     9001 gaaggacctg gaggacgact gggggctgac cggcgggcat ctcttccacg gcgaagagtc
     9061 gctcgatcag atcttcacga tgcggccggt gctcggctgg gcgcgttacc gcacgcccgt
     9121 gcgcgggctg tacctgtgcg gctcgggcac gcaccccgga ggcgggctca ccggcgccaa
     9181 cggccgcaac gccgcacgcg cactgctcgc cgaccggcgc tagctcggct caccgacgcc
     9241 accggctcga gcgcccgcaa ccgggaagga aaccccatca gacggcgtcg cttctcgacg
     9301 ccatgttgaa gtcgcgcgcc cgcaccgccg gaaccaccat gcggccggcc cgcaccgagg
     9361 cgctcatcgc ctcgacgttg cgcagcagcg tcgccgggcc gtcgttgaag cggaaattgt
     9421 tgaccggcgc gacgatcttc cccttctcga ccagccacac gccgtcgcgc gtcagcccgg
     9481 tctgctgcag ggtctgggga ttgagcgtgc ggatgtacca gaagcgggtg acgatgagcc
     9541 cgcgtgtgca cgatgcgacg agatcgtcga gcgtgccgct ccccccctcg agaatcaggc
     9601 tcccgggata cggcaccggg ggcgcgttgg tcttccgcgc ccagtagcga tcgacgctca
     9661 ggttcctgag cgtccccttc tcgacccagg tcaccctggc cgcgggaagc ccctggttct
     9721 gcgcgccggc gccgaaggcc cctccgccac ctccgccgaa ccccagcggg acgatccacg
     9781 gccggcccgg aacgcgcgcg tcgaacggat ccgatcgcag cgtcaccatc tcggcgaaca
     9841 ccttctcgcc gacccggttc ccgccccccg gcttcgagaa gacgctgcgc ccctcgtccg
     9901 cggcgcgggc ctgcagcccc ccgatcatgg tgccgaggag atcggccacc gcctggggct
     9961 cgaggaggac cgtgtagcgt cccggctcga ggtcttttgg cttcgcggag gcgagagcct
    10021 tcttcgcggc ggtggcggcg atctcgccgg tcttgagatc ggcgaggcgc gtcgccgtgc
    10081 ggcccgccca gccggagccg gtgccgtccg gcgtccgcat cgtcgtcgag aacgacgctt
    10141 cggtcgcgcg ctggaagccg aagagcccct tgcggttggc aatcgccgag gaggtgatgt
    10201 cagtggagaa gaagcctgag gcggcgaggc tcgcgccgcg cgccgcttcg atcgccgccg
    10261 cggccccgtc gcggcgcatc tccggcccgg ccgcggcggt cgccgcgtcg aacgcctcga
    10321 tcgccggata ggtctgcgga ccgagctctt cgacccactc cgggctctcc ggggcgagcc
    10381 cgaggatctc ctcgctgcgc cgcacggccc gctcgagcga tgcggggtcg gtctcgctca
    10441 cctgcaccgt gccactcttc ccccgcttgc ggctggtgat ggtcagcgcg agggtcgtcg
    10501 cgatccccgc cgtggtgaca tccgaagcgg cgaaacgcgt gtgcgccgac tccctcgcct
    10561 cgatcgtgac ctcgcactcc tccaccctcg agaaggagag gatgcgatcg gcgagcgcgc
    10621 gcgcctcttt cgccgtcaag gtggccatgg tccgccgtcc ctcactcggt gcgcagcacg
    10681 ttgacgcgcc ggaagcgggc cggcggacag ccgtggctca tggcattcac ctgttggggc
    10741 tgccccttgc cgtcgttctg cagcccgacg ttctcccagg tcgcatcacc gccgagacca
    10801 tcgcacgcct gccagaaatc gggggtgcgg ctctggtacg cgacgtcggc cagcatccgg
    10861 ccgaccttcc cccccttgat ctcccagaag acatcaccgc cgaactggaa gttgtagcgc
    10921 tgctggtcga tcgaccagga gccgcggccg tcgatcagga tcccttcgtc caccccggag
    10981 acgagatcgt cgaggctcac cggttcctgc gccggctcga gccagacgtt cggcatccgc
    11041 tggaacggga ccgaggcgta cgagtcggcg taacagcagc cggtcgactc ctcctgcccg
    11101 atgaggtgcg cctggtcgcg gatcgtctgg tagccggaga agatcccctt cttgaacagc
    11161 gggaactgcc gcgtcttcac gccgtcgtcg tcccaaccac atgtcgaaag gctctcggga
    11221 cggacgcgat cgccgttgac gttgacgagg tcggacccga cgcggaactt gccgagcttg
    11281 tccggggtca ggaaggtggt gccggcgagg tccgcctcgt accccagggc gcgatcgagc
    11341 tcggtcgagt gcccgagcga ttcatgaatc gtcagcccga gatggctcgg catcagcacg
    11401 aggtccttcg tccccggatc gaccgacggc gccttgaggt gggccaccgc ctcctcggcg
    11461 atccgccggg cgtggtcggc gtagcgggcc cgctccaccg cctcccagcc tgcggcgacg
    11521 gcatgcggcc ggtaggtgcg cgatttggtc ttccgccctt tctcgacggc ggtggcggtc
    11581 agctcg
//
LOCUS       DASRIQ010000584         5612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1544733, whole genome shotgun sequence.
ACCESSION   DASRIQ010000584 DASRIQ010000000
VERSION     DASRIQ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544733"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..760)
                     /locus_tag="VFD06_14455"
     CDS             complement(83..760)
                     /locus_tag="VFD06_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04782.1"
                     /translation="MSDASKRSSALLDAQDALLLLRSRSLSLGLIRRIAATPEFVRND
                     RVRAAIVLHPRTPRVLAMGLLPHLRWHDLLRVTTTPGCAAPLILGAERLLALRVPDLA
                     LGERISLARAASETLVKTVRRETSPLVVKALLENPRFRSEDALFIAERPETPAATLRL
                     LAESPRWRGRSDLRHAIAVHPATPPQAALRLVAAMDGPEIGRLLQSGRAPALVRLAAE
                     RRLSGRG"
     gene            806..2056
                     /locus_tag="VFD06_14460"
     CDS             806..2056
                     /locus_tag="VFD06_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN04783.1"
                     /translation="MTLRRRLLVPMLLVALSLAVALLAGEAGVRLLGLQGREKVRVVP
                     GRGITFEPRAAYRHVKEGRASGRFNGEGFRDRERRIEKPPDGYRILVLGDSFVEALQV
                     ELDEAFPAVLEDRLRLRPGRGSIEVLAMGQSGFGTASELMRYLEFGAAYRPDVVLLAF
                     YAGNDVRDNSRLLSEDAPAFYYTLDQDGTLRLDRSCMEALERERASQGLAGWLQDESR
                     LVSLVSERLYLWRHARRSARGAAAPSHAVAGRGSAPVDPRDDANVYLEDAGPAWREAW
                     EVTGALLERFAEETAARGSRLAIVSVGTAEQVEEDLGRAVLAAGPGRDLDRPDRRLEA
                     MAARRGTPCLLLAPGLRAAEAAGSGPVYGLGGRRGGHWNSEGHRRAALLLEPFLEEAD
                     LLPEAGSQAALGAARAFTAPSRGR"
     gene            2119..4008
                     /locus_tag="VFD06_14465"
     CDS             2119..4008
                     /locus_tag="VFD06_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2362..2364,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN04784.1"
                     /translation="MKKYVRLTEPMVREHGRLRPATWDEALDRAAEGFRRVRDGRGGQ
                     GIGFFSCSKATNEVNYLAQKFARTVLGSNNIDSCNRTUHAPSVVGLTTVFGAGGGTSS
                     YREVEETDLVLLWGSNAREAHPIYFHHVLRGVRRGARLVVVDPRRTVSAQWADRWLGL
                     DVGSDVALANAMGREILAHGLENRAFIARATRDFEAYRDGVQKYTLEYAERETGIPAE
                     AIRETALAYARADRAQICWTLGITEHHNAVDNVFALINLALLTGHVGRYGSGLNPLRG
                     QNNVQGGGDMGALPNKLPGGQDVEDPEHRAKFERAWKATIPPKAGLHLTQMFEAMEHG
                     DLKAIYVLGENPAQSEADQERALKLLSGLDHIVVQDMFLTRTAEMAHVVLPAGASWCE
                     SEGTVTSSERRVQRVRKALDPPGQAREDIVILTAIARRLGADWDTPAAEQVWDELRSL
                     SPWHGGMSYERLESLGGIPWPCPDESHPGSPFLHARLWKEPIEGPKAPFHAVEHDPPV
                     DRLSDEYPIRLTTGRRLDSYNTGVQTGGYTSPLRRGESLDIAPEDGARLGLADGTPAR
                     AVSRRGAVVVPVRYDPSLRPGLAFMTLHFQDEVATNLLTIDATDPKSGTAEFKATAIR
                     IEKVA"
     gene            4030..>5612
                     /locus_tag="VFD06_14470"
     CDS             4030..>5612
                     /locus_tag="VFD06_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZN04785.1"
                     /translation="MDLHLIPGAVPSAEEKEAVDRVAGRPATGWEGGARRPKSEGHTA
                     TGGHAARGRRHLLLPALWAVQARAGFISPGAVNYICQRLSVPPADAYGVASFYSMFST
                     EKRPLTIAHVCDDLACRLAGAETICAELETRLGPAGAPALEGAAAWHRSPCLGLCERA
                     PALLLQAAGPNRFDRALAPVDADRIVAALGEETQARAPVARPSKADAAASGPEAGRSG
                     SPSAPQTLDARRAGLRILKRVGRVDPESLDDYRAHGGYLALRRALDAGPEKVLREVLD
                     SRLMGRGGAAFPTGRKWDAVARNPVTPHFVVCNADESEPGTFKDRLLLEEDPFAVVEA
                     MTIAGYATGSERGWLYVRGEYPLALQRFETAVDRARERGYLGASVLGEPFAFDIEVRR
                     GAGAYICGEETALFNSIEGLRGEPRNKPPFPVQAGLFGKPTAINNVETLANLPEILLE
                     GGPAFAGVGTEQSTGTKLFCVSGSIARPGVYEVPFGVTLGGLLDLAGGPRAGRSLRAV
                     LLGGAAGSFVGEEALATPLT"
BASE COUNT          762 a         2001 c         2090 g          759 t
ORIGIN      
        1 catggggccg caacccccat gcgcccttcg ggttcgcggc gccgcgaacg caacccatgc
       61 caagactcgg acaggctcct actcatcctc gcccggaaag acgcctttcg gcggccagcc
      121 ggacgagggc cggagcgcgc ccgctctgga gcaggcggcc gatctccggg ccgtccatcg
      181 cggcgacgag ccgcagcgcc gcctgtgggg gcgtcgccgg atgcaccgcg atcgcatgcc
      241 tcagatcgct gcggccgcgc cagcgcgggc tctccgccag gagccggagc gtcgcggccg
      301 gcgtttcggg tcgctccgcg atgaacagcg cgtcctcgga gcggaatcgg gggttctcga
      361 ggagagcctt caccacgagc gggctcgttt cgcgtcgcac ggtcttcacc agggtctccg
      421 aggcggcgcg cgcgagcgag atccgctcgc cgagcgcgag atcgggaacc cgcagcgcga
      481 gcaggcgctc cgccccgagg atgagcggcg cggcgcaccc gggggtcgtc gtcacccgga
      541 gcaggtcgtg ccagcggagg tgcggcagga gtcccatggc gagaacccgg ggagtgcgcg
      601 ggtggagcac gatcgccgcc cgcaccctgt cgttgcgtac gaactccggc gtggcggcga
      661 tgcgccggat gagcccgagg ctgagcgacc gggagcgcag gagcaggagc gcgtcctgcg
      721 cgtcgagcag ggcggacgat cgtttcgagg cgtcgctcac ggtgggcgac tctaacttag
      781 aatcggtgct ctgctacagc acgtcatgac cctccgccgg cgcctcctcg tcccgatgct
      841 cctcgtcgcc ctgtcgctcg ccgtggccct tttggcaggg gaggccggcg tccgcctgct
      901 cggccttcag gggcgggaga aggtgcgcgt cgtccccggc cgtggaatca ccttcgagcc
      961 ccgggcggcc taccggcacg tgaaggaagg tcgggcgtcg ggccgcttca acggggaggg
     1021 gttccgcgac cgggagcgcc gcatcgagaa acctccggac ggctaccgca tcctcgtcct
     1081 gggggactcc ttcgtggaag cgctccaggt cgagctcgac gaggcgttcc ccgcggtgct
     1141 cgaggatcgc ctgcgcctgc gcccggggcg cggatccatc gaggtgctgg cgatggggca
     1201 gtcgggattc ggcaccgcct cggagctgat gcgctatctc gagttcggcg ccgcgtaccg
     1261 gccggacgtc gtcctcctgg cgttctacgc ggggaacgac gtgcgcgaca attcgcgact
     1321 cctcagcgaa gacgcgccgg ccttctacta cacgctcgac caggatggga cgctgcgcct
     1381 cgatcgctcg tgcatggagg cgctggagcg ggagcgcgcc tcgcaggggc tggccggctg
     1441 gctgcaggac gagtcgcggc tcgtgtcgct cgtctccgag cggctgtatc tctggcgcca
     1501 cgcgcgccgc tccgcccggg gcgcggcggc gccgtctcac gctgtcgcgg ggaggggatc
     1561 ggctcccgtc gatccgcgcg acgacgccaa cgtctatctc gaggatgcgg gccctgcgtg
     1621 gcgcgaggca tgggaggtga ccggggcgct cctcgaacgg ttcgccgagg agaccgcggc
     1681 gcgcggcagc cgcctggcga tcgtgtcggt cgggaccgcc gagcaggtgg aggaggacct
     1741 cgggcgcgcg gtgctcgcgg ccggacccgg ccgggatctg gatcgtcccg atcggcgcct
     1801 cgaggcgatg gcggcgcggc gcgggacgcc gtgcctgctg ctcgcacccg gactccgcgc
     1861 cgccgaagcg gccggatccg ggccggtcta cggactcggg gggcggcgcg gcgggcactg
     1921 gaactccgag gggcaccgcc gggcggccct gctcctcgag cccttcctcg aagaggccga
     1981 cctgcttccg gaggcgggct cgcaggccgc gctcggagcc gcgcgcgcct ttacagcccc
     2041 gtcccggggg agataaccta ctcgaggccg gcggcggcgc gcgatgggcg cgggccgtca
     2101 cgccggagtg gagcggtcat gaagaagtac gtccggctga ccgagccgat ggtgcgtgag
     2161 cacggccgcc ttcgtcccgc cacctgggac gaggcgctcg atcgcgccgc cgaaggtttc
     2221 cggcgggtgc gcgacgggcg cggcgggcag gggatcgggt tcttctcctg ctcgaaggcg
     2281 accaacgagg tgaactacct cgcgcagaag ttcgcccgca ccgtcctcgg cagcaacaac
     2341 atcgactcct gcaaccgcac ctgacacgct ccttccgtcg tcggtctgac gacggtcttc
     2401 ggagccgggg gcggcaccag cagctaccgc gaggtcgagg agaccgatct cgtgctcctc
     2461 tggggcagca acgcccgcga ggcgcacccg atctatttcc accacgtcct gcgcggcgtg
     2521 cgccgcggcg cgcggctggt ggtggtcgat ccgcggcgga cggtgtcggc gcaatgggcg
     2581 gaccggtggc tcgggctcga cgtgggatcc gacgtggcgc tggcgaacgc catgggacgg
     2641 gagatcctcg cgcacgggct ggagaaccgc gccttcatcg cgcgcgcgac acgggacttc
     2701 gaggcgtacc gcgacggcgt gcagaagtac accctggagt acgccgagcg cgagaccggc
     2761 atcccggcgg aggcgatccg cgagacggcg ctcgcctacg cgcgggccga tcgcgcccag
     2821 atctgctgga cgctcggcat caccgagcac cacaacgcgg tggacaacgt cttcgccctc
     2881 atcaacctgg cgctcctcac cggccacgtc ggccgctacg gatcggggct caacccgttg
     2941 cgcggccaga acaacgtgca ggggggcggc gacatggggg cgctcccgaa caagctgccg
     3001 ggcgggcagg acgtcgagga cccggagcac cgcgcgaagt tcgagcgcgc ctggaaggcg
     3061 accatccctc cgaaggcggg cctgcacctg acgcagatgt tcgaggcgat ggagcacggg
     3121 gatctcaagg cgatctacgt gctcggggag aacccggcgc agtcggaggc ggaccaggag
     3181 cgcgcgttga agctgctctc cgggctcgac cacatcgtcg tgcaggacat gttcctgacc
     3241 cgcaccgccg agatggcgca cgtcgtcctg ccggccggcg cctcctggtg cgagtcggag
     3301 gggacggtca cctcgagcga gcggcgcgtg cagcgggtgc gcaaggcgct cgatcctccc
     3361 ggtcaggcgc gcgaagacat cgtcatcctc acggccatcg cccgcaggct cggcgccgac
     3421 tgggacaccc cggcggccga gcaggtgtgg gacgagctgc ggtcgctgtc gccgtggcat
     3481 ggcggcatgt cgtacgagcg gctcgagtcg ctcggcggga tcccctggcc ctgcccggac
     3541 gagtcgcacc ccggaagccc gttcctgcac gcgcggctct ggaaggagcc gatcgagggg
     3601 cccaaggctc cgttccacgc ggtcgagcac gatcctccgg tcgatcggct gagcgacgag
     3661 tacccgatcc gtctcacgac cggtcgccgg ctcgactcct acaacaccgg ggtgcagacc
     3721 ggcggctaca cctccccgct gcggcgcggc gaaagcctgg acatcgcgcc ggaggacggc
     3781 gcgcgcctgg gcctcgcgga cggaaccccc gcgcgggcgg tgtcgcggcg cggcgccgtc
     3841 gtcgtgccgg tccgctacga cccgtcgctg cggcccggcc tcgccttcat gacgctgcac
     3901 ttccaggacg aggtcgccac caacctcctc accatcgacg ccaccgatcc gaagtcgggg
     3961 acggcggagt tcaaggcgac cgccatccgc atcgagaagg tcgcctgatc cgagtggggg
     4021 cctcctaagt tggatctgca tctcatcccg ggcgccgtcc ccagcgccga ggagaaggag
     4081 gcggtggacc gcgtggccgg ccgccccgcc accgggtggg aggggggcgc ccgccggccg
     4141 aagagcgagg ggcacacggc cacaggaggt cacgcggcgc gcggccggcg ccatctgctg
     4201 ctgccggcgc tgtgggcggt gcaggcgcgc gccggattca tctcgccggg ggcggtgaat
     4261 tacatctgcc agcgcctctc cgttccgccg gccgacgcct acggcgtcgc gtcgttctac
     4321 tcgatgttct cgaccgagaa gcgcccgctg acgatcgccc acgtctgcga cgatctcgcc
     4381 tgccggctcg ccggcgcgga gacgatctgc gcggagctcg agacgcgcct gggtccggcc
     4441 ggcgcgccgg cgctcgaggg ggcggctgcg tggcaccgct cgccgtgcct cggactgtgc
     4501 gagcgggcgc cggcgctcct cctccaggcg gccgggccga accgcttcga tcgcgccctc
     4561 gctccggtcg acgccgaccg gatcgtcgcg gcgctcggag aagagacgca ggcccgcgct
     4621 cccgtggccc ggccttcgaa agcggatgcg gcggcgtccg gaccggaggc gggacgctcc
     4681 gggtccccgt cggcgccgca gaccctcgac gcgcgccgcg cggggctcag gatcctgaag
     4741 cgcgtcggac gcgtcgatcc ggagagcctc gacgactacc gggcgcacgg cggctacctg
     4801 gcgctgcgcc gcgccctcga cgccggcccc gagaaggtgc tgcgcgaggt gctcgactcg
     4861 cgtctcatgg ggcgcggcgg cgccgccttc ccgaccggtc gcaagtggga cgcggtggcg
     4921 cgcaacccgg tgacgccgca cttcgtcgtc tgcaacgccg acgagtcgga gccggggacc
     4981 ttcaaggacc ggctgctcct cgaggaggat ccgttcgccg tcgtcgaggc gatgacgatc
     5041 gccggctacg ccaccggctc cgagcgcggc tggctctacg tccgcggcga gtacccgctg
     5101 gccctgcagc gtttcgaaac ggccgtcgat cgcgcccgcg agcgcggcta cctcggcgcg
     5161 tcggtcctcg gcgagccgtt cgccttcgac atcgaggtcc ggcgcggcgc cggggcgtac
     5221 atctgcggcg aggagacggc gctgttcaac tccatcgaag ggctgcgcgg cgagccgcgc
     5281 aacaagccgc cgttcccggt gcaggcggga ctgttcggca agccgaccgc catcaacaac
     5341 gtcgagacgc tcgccaacct gccggagatc ctcctcgaag gagggccggc gttcgccggc
     5401 gtcggaacgg agcagtcgac cgggacgaag ctgttctgcg tgtccggctc gatcgcgcgc
     5461 ccgggggtgt acgaagtccc gttcggcgtc acgctcggcg ggcttctcga tctcgccggc
     5521 gggccgcgcg cgggccgatc gctccgggcg gtgctcctcg ggggcgcggc cggatcgttc
     5581 gtcggggagg aggcactcgc gaccccgctc ac
//
LOCUS       DASRIQ010000585         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2568144, whole genome shotgun sequence.
ACCESSION   DASRIQ010000585 DASRIQ010000000
VERSION     DASRIQ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2568144"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFD06_14475"
     CDS             <1..452
                     /locus_tag="VFD06_14475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04786.1"
                     /translation="REAGLEQAAAVAFEQAVRTDADLAPAWGALGTSLLRAGDAEGAS
                     AALQTALRLDPHDVVARNNLGTILLQAGAADAAEEMFRTAIRDAPWLAAAHANLGVVA
                     RRRGDLATAERELRAALRLDPGNDDARFNLATVLSEKGLPAPPPPAR"
     gene            536..>2931
                     /locus_tag="VFD06_14480"
     CDS             536..>2931
                     /locus_tag="VFD06_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04787.1"
                     /translation="MRPRPGVLVRRRSSFTVTIAILKAVHTRWFRTGRAIAAAALLLV
                     LPTLAWGHCRSASSCSSGTCLFSASDSWISGTDCLTPPDSPTESWILSTGHTMLVDTA
                     WTITGPATVLGTLSFDTDTAQRDENGYRNLVITTAAGEALICSATGSMVLRASDRLRF
                     DTRAGIATLIHSNGCRLDLRGSTFATSVAVVEAGDDDDSPCGHATPVGREWTITPIAG
                     IETAKVGRRVIFTSGPLVNRHFEIAAVGPESLTLCSHLADVLSLGERLTPRLSGGAPP
                     GPGTAGHHATPAITAGNDACTGPGSPHPCCSGHLAGTCTDGPDPAAGDTFEIVDDGTI
                     DQWAGTNGWRFADGGDGTVLCNGGGCTGSDPFPIVQYMNLSGFGGGGNNTGIAAASFS
                     VRLADQFVPDFVGNNLHGFRSDYGIIFRGIKDNLIAWNAIHDSDRPPGLSDNYCQLGV
                     HQHKRSENGRVDTPAAGVTIRNNVAWGTKQCAIEINDRLNRADEPPAAYQAYDIRVDD
                     NLVFGGCAVGPGRECAAINLHSCRNCEVRRNVCYDMHNADHTNGVCVFAGGSASNDGS
                     DVSYNWLTNGSGIGIRCQDDVSPSSFANCLHVGITGNYISHFRVGGGLGGRWFGNIVK
                     NFGLNHPQGGSGIDNPIRAYGSYIGLDEEVATTQACTGSGDRCGRVGIAWYRGPGNNG
                     RFDVAASDLLFGKMSENPGGLSRAALDNSRFNQQAVADFNGAVSHITHDNRATTNFGS
                     ALTFNFSPVGPATPVTWSISDVLAAYKRDDRLVNCTTAANVAERIGNAYSLLTSNPVE
                     G"
BASE COUNT          489 a         1057 c          937 g          448 t
ORIGIN      
        1 atcgcgaggc ggggctggag caggcggccg cggtggcctt cgaacaggcg gtccgcacgg
       61 atgcggatct cgcacccgca tggggggccc tcggaacctc cctgctgcgg gcgggtgatg
      121 ccgaaggggc cagcgcggcc ctgcagaccg cgctccgcct cgatccccat gatgtcgtcg
      181 ccaggaacaa tctgggcacg attctgttac aggccggcgc agcggacgcg gccgaggaga
      241 tgttccggac ggcgatccgg gacgctccgt ggctggcggc ggcgcacgcc aacctggggg
      301 tcgtggcgcg acggcgcggc gacctggcga ccgcggagcg ggagctgagg gccgccttga
      361 ggctcgatcc cgggaatgac gatgctcgtt tcaatctcgc cacggttctg agcgaaaaag
      421 ggctgccggc tccgcccccc cccgctcgat gacgcgggaa tccgctccca tggcggcttg
      481 tgcagatccg accttgcatc agcgactggg acgggtcact attacgagac cctgcgtgag
      541 gccgcgtccc ggggtgctcg tgcgtcggag gtcatccttc accgtcacca ttgccatttt
      601 gaaagccgtg catactcgat ggttccgaac cggccgagcg atcgcggccg ccgccctgct
      661 gctggttctt ccgactctgg cctggggcca ttgtcgttcc gcctcgtcct gctcgtccgg
      721 cacctgcctc ttcagcgcct cggacagctg gatcagcggc accgactgcc tcacgcctcc
      781 ggactccccc accgagagct ggatcctgtc gaccggccac accatgctcg tggacaccgc
      841 ctggacgatc accggtcccg ccacggtcct cggcacgctg tccttcgaca ccgataccgc
      901 acagcgcgac gagaatggct accggaacct ggtgatcacc accgcggccg gggaggccct
      961 gatctgcagc gcgaccggct cgatggtcct gcgcgcctcg gaccgcctcc gcttcgacac
     1021 ccgcgccggg atcgcgaccc tgatccattc caacggctgt cggctcgacc ttcgcggatc
     1081 cacgttcgcg acctccgtcg cggtggtgga ggccggggac gatgacgact caccgtgcgg
     1141 ccatgcgacg ccggtcgggc gcgaatggac catcactccg atcgccggca tcgagaccgc
     1201 gaaggtcggt cgccgcgtca tcttcacatc cgggccgctc gtgaaccgcc atttcgagat
     1261 cgctgcggtc ggccccgaga gtctcacgct ctgctcgcat ctcgcggacg ttctcagcct
     1321 gggcgagcgt ctgaccccgc gcctctcggg tggcgccccg ccgggacccg gcaccgccgg
     1381 acaccacgcc accccggcca tcaccgccgg aaacgacgcc tgcaccggcc ccggctcacc
     1441 gcacccatgc tgctccggcc acctggcagg cacgtgcacg gacggccccg atccggcggc
     1501 gggcgacacc ttcgaaatcg tcgacgacgg caccatcgat caatgggccg gaacgaatgg
     1561 ctggcggttc gcggacggcg gcgacgggac cgtcctctgc aacggcggcg gctgcaccgg
     1621 atcggatccg ttccccatcg ttcagtacat gaacctctcg ggcttcggag gcggtggaaa
     1681 caacacggga atcgccgccg cctcgttctc cgtccgattg gccgatcagt tcgtgcccga
     1741 tttcgtgggc aacaacctgc atggcttccg ctcggactac gggatcatct tccgcggcat
     1801 caaggacaac ctcattgcct ggaacgccat ccacgacagc gatcgacccc ccggattgag
     1861 cgacaactac tgccagctgg gagtgcacca gcacaagcgc tccgagaacg ggcgggtcga
     1921 caccccggcg gcgggcgtca ccatccgcaa caacgtcgcc tgggggacga agcagtgcgc
     1981 cattgagatc aacgacaggc tgaaccgcgc cgacgagcca ccggcggcgt accaggcgta
     2041 cgacatccgc gtcgacgaca acctcgtgtt cggagggtgc gccgtcggtc ccggaagaga
     2101 atgcgcggcc atcaacctgc actcgtgcag gaactgcgag gtgcggcgga acgtctgcta
     2161 cgacatgcac aacgccgatc acacgaacgg cgtctgcgtc ttcgccggcg gctcggccag
     2221 caacgacggc agcgacgtct cctacaactg gctcacgaac ggcagcggga tcgggatccg
     2281 ctgccaggac gatgtgtccc cgtccagctt cgcgaactgc ctgcacgtcg ggatcaccgg
     2341 caactacatc tcgcacttcc gcgtcggggg agggctgggc ggccgctggt tcggaaacat
     2401 cgtgaagaac ttcggcctga accatccgca ggggggctcc ggcatcgaca atccgatccg
     2461 cgcttacggc tcctacatcg gcctcgacga ggaggtcgcg acgacccagg cctgcacggg
     2521 cagcggtgac cgttgcgggc gcgtcggaat cgcctggtat cgcggccccg gcaacaacgg
     2581 ccggttcgac gtggcggcgt ccgacctgct cttcgggaag atgagcgaga acccgggagg
     2641 tctctcccgc gccgccctcg acaacagcag gttcaaccag caggcggtcg ccgacttcaa
     2701 cggcgcagtc tcgcacatca cccacgacaa ccgcgccacc accaatttcg gatccgcgct
     2761 cacgttcaac ttttcgcccg tgggtcccgc gaccccggtg acatggtcga tttcggacgt
     2821 cctggccgcc tacaagcgcg acgaccggct cgtcaactgc acgacggccg cgaacgtcgc
     2881 ggagcgcatc ggcaacgcct acagcctgct gacctccaac ccggtggagg g
//
LOCUS       DASRIQ010000586         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3636779, whole genome shotgun sequence.
ACCESSION   DASRIQ010000586 DASRIQ010000000
VERSION     DASRIQ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3636779"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFD06_14485"
     CDS             <1..684
                     /locus_tag="VFD06_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HZN04788.1"
                     /translation="ETLPLEPQTVADFYRRYLEALRSLGIPARVRHPKPNEVANAIRF
                     DDDTVHGSYDAESVGRFRQALLQADRLLKEFRGRFIGKCSPVHFWWGAFDLACTRFNG
                     RPAPPHPGGIPNLPDRVTRESYSHECISAGWWPGNGGGPVREPAFYAYAYPEPVGCPQ
                     AAIRPAAAAYHAALREWILPYDAVRRSSMPDEMVLDFFQSTYEAAANLGAWDRAALER
                     TPLTPRPTA"
     gene            complement(662..1570)
                     /locus_tag="VFD06_14490"
     CDS             complement(662..1570)
                     /locus_tag="VFD06_14490"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZN04789.1"
                     /translation="MRHYLTLSPRQLPSQYLYDDLGSALFEAICRLPWYPLTRAEMRL
                     LAAHAPEILDRRVTSILELGAGSGLKLSALIRGAGPDRGRLDVHVVDVSAAALAETRR
                     ALAAFGDLRVVAHEATYETGLERLKSTPARGRRLMLFLGSNIGNFDPEGRDLFLRALR
                     AALRPGDRLLLGVDLIKPVRDLMLAYDDPLGVTAAFNLNLLSRINRELWGDFGLDRFA
                     HHVVWNAEQARIEMHLRSTTAQRIRIPGAALHLELREGETIWTESSYKYETSEIVNLL
                     ARGGFEVAAQWVDASGRFALTLSDAV"
     gene            complement(1690..>2542)
                     /locus_tag="VFD06_14495"
     CDS             complement(1690..>2542)
                     /locus_tag="VFD06_14495"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZN04790.1"
                     /translation="GLKRSPSGYRPRVDGAIPRTRWIDIGPGRATLGVDRSEAVYAWD
                     NECPRRAEPVRAFAIESGNVTNAAFMEFVASGGYRAARFWRPADWDWVRREDLRHPPF
                     WERREEGWTWRGLFEAIPLPPSWPVYVSHAEAEAYARWRGSRLPTEGEFQRAAFGTPG
                     GEERPFPWGETAPDAGRHGVFDFTSWDPAPAESHPAGRSAWGVDDLVGNGWEWTSTPF
                     APFPGFRPQATYPEYSADFFDGDHFVMKGASPATARELLRPTFRNWFRGRYPFMYATF
                     RCARDLA"
BASE COUNT          388 a          922 c          879 g          353 t
ORIGIN      
        1 gagacgctcc ccctcgagcc gcagacggtc gcggacttct accgccgcta cctggaggct
       61 ctgcgctcgc tcggcatccc ggcgcgcgtg cggcacccga agcccaacga ggtggcgaac
      121 gcgatccggt tcgacgacga cacggtccat gggtcgtacg acgccgagtc ggtcggtcgc
      181 ttccgccagg cgctcctcca ggccgatcgg ctgctgaagg agttccgggg acggttcatc
      241 ggcaagtgca gcccggtgca tttctggtgg ggggcgttcg acctggcgtg tacccgcttc
      301 aacgggcgcc cggcgccgcc gcatcccgga ggcatcccca acctgcccga ccgggtgaca
      361 cgcgagtcgt attcgcacga gtgcatcagc gccggatggt ggcccggcaa cggcggcggc
      421 ccggtgcgcg agccggcgtt ctacgcctac gcctaccccg agccggtggg gtgcccgcag
      481 gcggcgatcc gaccggccgc ggcggcatac cacgcggcct tgcgcgaatg gatcctgcct
      541 tacgacgcgg tgcgacggtc gtccatgccg gacgagatgg tcctcgattt cttccagagc
      601 acctacgagg ccgcggcgaa cctcggtgcc tgggaccgcg ccgcgctgga gaggacgccg
      661 ctcacaccgc gtccgacagc gtgagggcga aacggccgga ggcgtccacc cactgcgccg
      721 cgacctcgaa cccgccgcgc gccaggagat tcacgatctc gctcgtttcg tacttgtagg
      781 acgactccgt ccagatcgtc tccccctccc ggagctccag gtggagggcg gcgcccggga
      841 tgcggatgcg ctgcgccgtc gtgctccgca ggtgcatctc gatgcgtgcc tgctccgcgt
      901 tccagacgac gtggtgcgcg aagcgatcga gaccgaagtc tccccacagc tcgcgattga
      961 tgcgcgacag gagattcagg ttgaacgccg cggtgacgcc gagcgggtcg tcgtacgcca
     1021 gcatcaggtc gcggaccggc ttgatcagat ccaccccgag caggagtcga tcgcccggac
     1081 gaagcgccgc gcgcaaggcg cgcaggaaca gatcacgccc ctccggatcg aagttgccga
     1141 tgttcgagcc gaggaacagc atcagccgcc tcccccgcgc gggcgtgctc ttcagcctct
     1201 cgagtcccgt ctcgtacgtc gcttcgtgag ccacgacacg gaggtcgccg aacgccgcga
     1261 gggcgcgacg ggtttcggcg agggcggccg ccgagacgtc cacgacgtgc acgtcgagac
     1321 gcccccggtc cggtcccgcg ccgcggatca gcgccgacag cttgaggccg ctccccgctc
     1381 ccagctcgag aatcgaggtc acgcggcggt cgaggatctc cggcgcgtgc gccgccagaa
     1441 gccgcatctc ggctcgcgtc agcgggtacc atggcagccg gcagatcgcc tcgaacaggg
     1501 ccgagcccag gtcgtcgtac aggtactgcg acggcaactg gcgcggtgac agcgtcaggt
     1561 agtgcctcac gtccgacccg aatcgggccg cggctgcggc ggccgcgacc tcgagcgcgg
     1621 gcgcccggca cgatggtgcc gacgccggag tcctgccgga cgcgcgaccc acgggcatcg
     1681 tcttcggcgt cacgcgagat ccctcgcgca gcggaacgtc gcgtacatga agggatagcg
     1741 cccccggaac cagttgcgga acgtcggccg caggagttcc cgcgccgtcg cgggcgaggc
     1801 ccccttcatg acgaagtgat cgccgtcgaa gaagtcggcg gagtattccg gataagtggc
     1861 ctgcggccgg aacccgggga agggggcgaa gggggtgctc gtccactccc agccgttgcc
     1921 gaccagatcg tccacgcccc aggcgctgcg tccggccgga tggctctcgg ccggcgcagg
     1981 gtcccaactc gtgaaatcga aaacgccgtg gcggcctgcg tccggcgccg tctcgcccca
     2041 cgggaacggg cgctcctctc ccccgggtgt tccgaacgcc gcgcgctgga actccccctc
     2101 ggtcggaagc ctggagccgc gccagcgcgc gtaggcctcc gcctcggcgt ggctgacgta
     2161 gaccggccac gagggtggaa gcggaatcgc ctcgaacaga ccgcgccagg tccagccctc
     2221 ctcgcggcgc tcccagaacg gcggatgccg gaggtcttcg cgtcgtaccc agtcccagtc
     2281 ggccggacgc cagaagcgcg ccgcccgata gccgccggag gcgacgaact ccatgaacgc
     2341 cgcgttggtc acattgccgg actcgatcgc gaaggcgcgc accggttcgg cgcggcgcgg
     2401 gcactcgttg tcccaggcgt agaccgcctc ggaccgatcg acccccagcg tcgcgcgccc
     2461 cggaccgatg tcgatccatc tcgtccgcgg gatcgcgccg tcgacgcgcg gccggtatcc
     2521 ggagggcgat cgtttcagcc ca
//
LOCUS       DASRIQ010000587         5288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1293018, whole genome shotgun sequence.
ACCESSION   DASRIQ010000587 DASRIQ010000000
VERSION     DASRIQ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293018"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1347
                     /gene="lpxB"
                     /locus_tag="VFD06_14500"
     CDS             142..1347
                     /gene="lpxB"
                     /locus_tag="VFD06_14500"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HZN04791.1"
                     /translation="MTPGARVLIVAGEASGDLYGGLLMRAMQEGGSRAAGNGDAAHPV
                     RFTGIGGEAMRGAGLEVLADAAILGVTGLVEVAGRFGAIWRAWRAAKRVLEDPARRPD
                     LAILIDYPDFNLRLAARARAAGVPVLYFVSPQVWAWRRGRIRQMAGRVDHMLVILPFE
                     EAIYREAGVPVTFVGHPLLDLVRTGRTRGQERARLGLDPDRPLVALLPGSRRNEVAAH
                     LPPLLDAADRLRAEFRDLQTLLTVAPTRSEAEIRAVAKTSGRRGPLPVLVQDDRYDAV
                     AAADAAVVVSGTATLETALLQVPMVIVYRMHPLTFALARRLTDLPHVGMPNLVAGRRI
                     VPELLQEECRGERIAAALRAILTDPGEAERMRDGLRSVRARLGEPGAIGRAARIAWDM
                     IAAARGGAR"
     gene            1356..2270
                     /locus_tag="VFD06_14505"
     CDS             1356..2270
                     /locus_tag="VFD06_14505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZN04792.1"
                     /translation="MATGPGQAERGVRGMTAFGGGLAERDGVRVAVEIKGFNNRVLDV
                     RLRLPAEAAGCEAEVRRRLQARLARGRVEVAATLTLPQGASPWLEIRDEVAAQYVQAA
                     RRLARRHKVGGTLQAAAILALPGVVQVGLVEGADKGLAEELVLGAFSEALGGFEAGRR
                     AEGTRLSADLLERLDAIERDTTAIGTDAGREPGRVAERLRQRLQPLLQDAALDPGRLA
                     QEVALLADRIDISEELVRLRGYLDQARGLLHGRETPTGKMLDFVMQEMNREANTIASK
                     AEALPICQAALRVRSAVETIREQVQNLE"
     gene            2267..2896
                     /gene="gmk"
                     /locus_tag="VFD06_14510"
     CDS             2267..2896
                     /gene="gmk"
                     /locus_tag="VFD06_14510"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZN04793.1"
                     /translation="MTTARGGLIVVSAPSGAGKTTLCRRLLEALPGLVFSVSHTTRAP
                     RAGERPGVDYHFVGRAEFEARRAAGEFLEWAVVGGELYGTSALAAESSLAQGLDVLLD
                     VDTQGAAAVRRLTPEAVLVFILPPGPEALRARLLARGSESEEGLERRLCLAAAEVARA
                     GEYDYQVINDDLDAAFDRLRAIVVASRCRTARLRERLETIAARFGDKSP"
     gene            3092..3373
                     /gene="rpoZ"
                     /locus_tag="VFD06_14515"
     CDS             3092..3373
                     /gene="rpoZ"
                     /locus_tag="VFD06_14515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZN04794.1"
                     /translation="MSQGGQMVRLDSIGNKFEFITIAAERCKQLQRGARARIDSISQK
                     PITIAQEEVLAGVIPYSYGPFPEEIPVQTEVSEVTTESYVLEEGGVTGE"
     gene            3366..3974
                     /locus_tag="VFD06_14520"
     CDS             3366..3974
                     /locus_tag="VFD06_14520"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HZN04795.1"
                     /translation="MSDAPPRVALGVSGGIAAYKAAEIVRGLRRGGADVHVLMTENAQ
                     RFITPLTLETLSGHRVLSDPWERAEGSDIQHITLARGLDLMLVAPATADVLARMAHGL
                     ADDFLTTFHLAVTAPIVVAPAMNVWMWEHAATRDNLRVLKDRGVAVIDPVAGELACGE
                     EGIGRMAEPETIVAAVFDRLKKKARGPRGRLRGVPSKDAASS"
     gene            3971..4648
                     /locus_tag="VFD06_14525"
     CDS             3971..4648
                     /locus_tag="VFD06_14525"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HZN04796.1"
                     /translation="MTAGPTREPFDPARFISNPSTGRMGYALATAARDRGAQVTLVSG
                     PTALEAPEGIDLVRVQTADEMLRATLNQFQGADLVLKAAAVSDYRPEVRSRAKLKKDV
                     FARRGRTGGKGDRAIAARLLPTTDILAEIGRRKKPHQILVGFAAESGDLLRNARLKLR
                     RKNLDLIVANRIGAAGEGFEADTNRALVLDRRGRRTDLPLMSKEAMASAILDLAQPLL
                     PRGQRSS"
     gene            4645..>5288
                     /locus_tag="VFD06_14530"
     CDS             4645..>5288
                     /locus_tag="VFD06_14530"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZN04797.1"
                     /translation="MSRDPAEARQALAARLEWLRILGVSEIAVKRTSVRSGSPEAAGA
                     VAAPARDAPPPPAPPRATDPPIASAPAGLPRLGLFGSDPQETPEEEPSQAVRTSAGRS
                     GAVAASPLLSATAPEGMDAATALRAIREDLGDCRRCRLCERRTQVVFGVGDPFARLLF
                     VGEGPGADEDAQGEPFVGRAGQLLTRMIEAMGLARGQVYIANVVKCRPPENRTPL"
BASE COUNT          699 a         1894 c         1997 g          698 t
ORIGIN      
        1 gaggcccgtc gatccgcggg ccgatcggga ggccatggag cgcgagcgcg ccgtgctcga
       61 agcgacgctc gagtcgctga cgacgagggc gcagcggctg gcggcgggcg agtccccctt
      121 cgccgccccc gaggcccgcg catgactccg ggggcgcgcg tcctcatcgt cgcgggggag
      181 gcctccgggg atctctacgg cggcctgctg atgcgggcga tgcaggaagg gggatcgcgc
      241 gcggccggga acggagatgc cgcgcacccc gtccgtttca ccggcatcgg gggcgaggcg
      301 atgcgcggcg cgggtctcga agtgctggcg gacgccgcga tcctcggcgt gaccgggctc
      361 gtcgaagtcg ccggccgctt cggcgcgatc tggcgcgcct ggcgcgcggc gaagcgggtc
      421 ctcgaggacc cggcgagacg ccccgatctc gccatcctga tcgactaccc agacttcaac
      481 ctgcgcctgg cggcgcgggc ccgcgccgcc ggggtgccgg tcctctactt cgtcagcccg
      541 caggtgtggg cctggcggcg cggccgcatc cggcagatgg cggggcgcgt cgaccacatg
      601 ctcgtcatcc tgccgttcga ggaggcgatc taccgggagg cgggggtgcc ggtgaccttc
      661 gtgggccacc cgctcctcga cctcgtccgg accgggcgca cccgcggcca ggagcgcgcc
      721 cgtctcgggc tcgatccgga ccgtccgctc gtcgccctcc tccccggcag ccgccgcaac
      781 gaggtcgccg cgcacctgcc gcctctcctc gacgcggccg accgcctgcg cgcggagttc
      841 cgcgatctgc agaccctcct gaccgtggcg ccgacgcgca gcgaggcgga gattcgcgcg
      901 gtcgccaaga cgtcgggccg gcgcgggccg ctcccggtgc ttgtgcagga cgatcgctac
      961 gacgccgtgg cggcggccga cgcggcggtc gtcgtctcgg ggaccgcgac cctcgagacg
     1021 gcgctcctgc aggtgccgat ggtcatcgtc tatcgcatgc acccgctcac cttcgctctg
     1081 gcgcgccgcc tcaccgatct gccacacgtc gggatgccca acctggtcgc ggggcggcgc
     1141 atcgtgccgg agctgctcca ggaggagtgc cggggggagc ggatcgccgc ggcgctgcgc
     1201 gccatcctga ccgatccggg cgaggccgaa cggatgcggg acggtctgcg atcggtgcgg
     1261 gctcggctcg gagagccggg ggccatcggc cgggcggcgc gcatcgcctg ggatatgatc
     1321 gccgccgcgc gcgggggcgc gcggtgaacc ctcggatggc gaccggaccg ggccaggcgg
     1381 agcggggcgt gcggggcatg acggcgttcg gcggcggtct cgccgagcgt gacggcgtgc
     1441 gggtcgccgt ggagatcaaa gggttcaaca accgcgtcct cgacgtgcga ttgcgccttc
     1501 ccgcggaggc ggccgggtgc gaggcggagg tgcggcgccg cctccaggcc aggctggcgc
     1561 gcggaagggt ggaggtggcg gcgacgctga ccctcccgca gggcgcgtcc ccctggctgg
     1621 aaatccgcga cgaggttgcg gcgcagtacg tgcaggcggc gcgtcgcctg gcgcggcgac
     1681 acaaggtggg tgggacgctg caggcggcgg ccatcctggc cctgccgggc gtggtgcagg
     1741 tcggtctggt cgagggcgcg gacaaggggc tcgccgagga gctcgtcctc ggggcgttct
     1801 ccgaggcgct cggcggcttc gaagcgggac ggcgggccga gggaacgcgg ctctccgccg
     1861 accttctgga gcggctcgac gccatcgagc gggacaccac ggcgatcgga accgacgccg
     1921 gccgtgagcc gggacgcgtg gccgagcgac tgcggcagcg gctccagccg ctcctccagg
     1981 acgcggcgct tgaccccggg cgcctcgcgc aggaggtcgc cctgctcgcc gaccggatcg
     2041 acatcagcga ggagctggtg cggttgcgcg gctacctcga ccaggcgcgc ggcctcctgc
     2101 acggtcggga gactcccacc ggaaagatgc tcgatttcgt catgcaggag atgaaccgcg
     2161 aggccaacac catcgccagc aaggcggaag cgctgccgat ctgccaggcg gcgctgcggg
     2221 ttcgatcggc cgtggaaacc atccgcgaac aggtgcagaa cctcgaatga ccacggcgcg
     2281 cggcggtctg attgtggtct cggcgccgtc gggagccggg aagacgacgc tctgccggag
     2341 gcttctcgag gctctgcccg ggctggtctt ctccgtctcc cacacgacgc gggcgccgcg
     2401 ggcgggggag cgtcccggcg tcgactacca cttcgtcggg cgcgccgagt tcgaggcgcg
     2461 gcgcgcggcg ggggagttcc tcgaatgggc ggtggtcggc ggggagctgt acggcacctc
     2521 ggcgctcgcc gccgaatcga gcctggcgca gggcctggac gtgctcctcg atgtcgacac
     2581 gcagggggcc gcggcggtgc gccgcctgac gcccgaggcg gtcctcgtct tcatcctgcc
     2641 gcccgggccg gaggcgctgc gggcgcgtct cctggcgcgc ggcagcgagt cggaggaggg
     2701 gctcgagcgg aggctgtgcc tcgcggcggc ggaggtggcg cgggccggcg agtacgacta
     2761 ccaggtgatc aacgacgacc tcgacgccgc cttcgaccgg ctgcgggcca tcgtcgtggc
     2821 gagccgctgc cgcaccgccc gtctccggga gcgcctcgaa accatcgccg ccaggttcgg
     2881 cgacaagtcg ccgtagcgca ctcaaaggcg catcccttca gagcaccacg ccgctcgccc
     2941 cgcaccgggc cccgtcgacg ctgcgccgtc cggcgcctct cgggcctgcc ggggctgggc
     3001 aatcaaccct tcctcggacg ggccgcttcg gatagaatga ccgcttctgt ccgccgcccc
     3061 gcccatgggg cgccggagcc cgggaggaac gatgagccag ggaggtcaga tggttcgcct
     3121 cgactcgatc ggcaacaaat tcgagttcat cacgatcgcc gcggagcgct gcaagcagct
     3181 gcagcgtggc gcccgcgccc gcatcgacag catctcgcag aagccgatca ccatcgcgca
     3241 ggaggaggtc ctggcggggg tcatccccta cagctacggt ccgttcccgg aggagatccc
     3301 ggtgcagacg gaggtcagcg aggtgaccac cgagtcgtac gtcctcgagg aaggtggcgt
     3361 cacgggtgag tgacgccccc ccccgcgtcg ccctcggggt gagcggcggt atcgcggcct
     3421 acaaggcggc ggagatcgtc cgcggcctgc gccgcggcgg ggcggacgtg cacgtcctga
     3481 tgaccgaaaa cgcgcagcgg ttcatcacgc cgctgaccct tgagacgctg tccgggcacc
     3541 gggtcctgtc cgatccgtgg gagcgcgccg aagggtcgga catccagcac atcacgctcg
     3601 cgcgcggatt ggatctgatg ctcgtcgccc ccgccaccgc cgacgtcctg gcgcggatgg
     3661 cgcatggact ggccgacgat ttcctcacca ccttccacct cgcggtcacc gccccgatcg
     3721 tggttgcgcc cgcgatgaac gtctggatgt gggagcacgc ggcgacgcgg gacaacctgc
     3781 gcgtcctcaa ggatcgcggc gtggcggtga tcgatcccgt cgctggcgag ctggcgtgcg
     3841 gcgaggaggg gatcggccgc atggccgagc cggagacgat cgtcgccgcg gtgttcgacc
     3901 ggctcaaaaa aaaagcgcgc ggcccccgcg gccgtctgag gggcgttccc tcgaaggacg
     3961 ccgcgtcctc gtgaccgcgg gccccacccg cgagccgttc gaccccgcgc gcttcatctc
     4021 caatccttcc accggccgca tgggctacgc ccttgccacc gccgcccgcg atcgcggcgc
     4081 gcaggtgacc ctcgtctccg gcccgaccgc gcttgaggcg ccggagggga tcgatctcgt
     4141 gcgcgtgcag accgccgacg agatgctgcg cgccacgttg aaccagttcc agggggccga
     4201 cctggtcctc aaggcggccg ccgtctccga ctaccgcccc gaggtgcggt cccgcgccaa
     4261 gctgaagaag gacgtcttcg cgcgccgcgg acggaccggc gggaagggag accgggcgat
     4321 cgccgcgcgg ctcctgccga cgaccgacat cctcgcggag atcggacggc gcaagaagcc
     4381 gcaccagatc ctcgtcgggt tcgcggcgga gagcggcgac ctgctgcgca acgcccggct
     4441 caagctgcgg cgcaagaacc tcgacctcat cgtcgcgaac cgcatcggcg cggcggggga
     4501 ggggttcgag gcggacacca accgcgccct ggtgctcgac cggcgcggcc ggcgcaccga
     4561 tctgccgctg atgagcaagg aggcgatggc ctccgccatc ctggatctcg cccagccgct
     4621 cctgccgcgg gggcaacgct cctcatgagc cgcgacccgg cggaagcccg ccaggcgctc
     4681 gcggcgcgcc tggaatggct gcggatcctc ggcgtctcgg agatcgcggt gaaacggacc
     4741 tccgtacggt cgggatcgcc ggaggccgcg ggcgccgtgg ccgcgccggc gcgggacgcg
     4801 ccccccccgc ccgccccccc gcgcgcgacc gatccgccga tcgcctccgc gcccgccggt
     4861 ttgccgcggc tggggttgtt cggctcggat cctcaggaga ctccggagga agagcccagc
     4921 caggcggtcc ggacgagcgc gggtcggagc ggcgccgtcg ccgcgtcgcc gcttctctcc
     4981 gcgacggcgc cggaggggat ggacgccgcc acggcgctgc gagcgatccg tgaggacctc
     5041 ggcgactgcc gtcgctgccg cctctgcgag cgccgcacgc aggtggtgtt cggcgtcggg
     5101 gaccctttcg cccgcctgct gttcgtcggc gaggggccgg gagcggacga ggatgcgcag
     5161 ggggagccgt tcgtcggccg cgccggccag ctcctgacca ggatgatcga ggcgatggga
     5221 ctggcgcgcg gccaggtgta catcgccaac gtggtgaagt gccgtcctcc cgagaaccgg
     5281 acgccgct
//
LOCUS       DASRIQ010000588         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3345251, whole genome shotgun sequence.
ACCESSION   DASRIQ010000588 DASRIQ010000000
VERSION     DASRIQ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3345251"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFD06_14535"
     CDS             complement(<1..366)
                     /locus_tag="VFD06_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04798.1"
                     /translation="MRKFIFSALLLAGLAAIVAPVAFAQEEKPFVLHGEVRTRFEYTN
                     NANDFTDSGTAPGLGSNDDQANYWPYRIRIAAEGHFTENISAWIEFQNAGVFGGDFTG
                     PVKNGTVFGFAADSGVSLYQ"
     gene            790..1563
                     /locus_tag="VFD06_14540"
     CDS             790..1563
                     /locus_tag="VFD06_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04799.1"
                     /translation="MNRFTPVALLLACVMVFAIFGCSGQGNQPAPEQTAEQAPPAAEP
                     PYQDQAAAPVPPPAAPAPSRSANTIPEERQPARPAPAVPSTVTITVPAGTQLTLGFPA
                     EISSETAQPGDAVVATLKESIVIGDRVAFPEGSQVRGTVTDVKPASKGFKDTGGAISM
                     QFKSVVAPNGTKSTIAAGFSKVAEGSAAKKGAIIGGSAVGGALLGKVLDKSTGGGAAV
                     GAAIGTAVAGTTKGREAKIKTDDMIAVPLEQSAKVVLPR"
     gene            complement(1686..2156)
                     /locus_tag="VFD06_14545"
     CDS             complement(1686..2156)
                     /locus_tag="VFD06_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04800.1"
                     /translation="MNKTMLMFGAAAFLVSAMLATPAHAQAVKEKSCTDEASGVSWDC
                     DYKLKSVTPGTPVSFTVNYSCNGACGPVLSFGLQSEAFSPAGCSGHLVGGRRLPGALE
                     LTFAFDSVKVAGVSGNALANAHFMMNVMATDASGAPVIVSLPVDVRLNEFENKK"
     gene            complement(2400..>3465)
                     /locus_tag="VFD06_14550"
     CDS             complement(2400..>3465)
                     /locus_tag="VFD06_14550"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04801.1"
                     /translation="LRRDLDASLSACRRAAEIAPDSPRVLMALAETLRETGAYDSAMT
                     LYGQAADLLHDSPLPQLGGAATLARSGAFSIAGRTYRMVLDRFPDVRLRALEGAAAVR
                     VVAGDYEGALDLYQEVDLPENAALPTLLSLYGKGYALLKLDRGAEAEYFLSLVVERVP
                     TEYDGPARGREMLFLAYDGLADWYSGRGRQDRVTALLREAAARPLAPTRMARRLAPLL
                     HERGKDSEAATLLERALLGADPGEDRLEITDSALLLARLRSSEGRRAIPSGSSARRAL
                     DAAARLVDAKAPAAACYRMARAYGLARDAEASLSFLGRSRDGGYMPAEQAATDPAFTI
                     LRQRPEFQRFLAPESKNDRS"
BASE COUNT          553 a         1178 c         1224 g          510 t
ORIGIN      
        1 ctggtagagg ctgacgccgg agtcggccgc gaagccgaac accgtgccgt tcttcaccgg
       61 gccggtgaag tcgccgccga acacgccggc gttctggaac tcgatccagg cactgatgtt
      121 ctcggtgaag tgcccctcgg ccgcgatccg gatgcggtac ggccagtagt tggcctggtc
      181 gtcgttcgac cccaagccgg gggccgtgcc ggagtcggtg aagtcgttcg cgttgttggt
      241 gtactcgaac cgcgtgcgga cctcgccgtg caggacgaag ggcttctcct cttgcgcgaa
      301 ggcgaccggc gcgacgatgg ccgccaggcc cgcgagcagg agcgcactga agatgaactt
      361 tctcattccc gatgatcctc cctgtcatta aggaaaagcc gactgcgatg aagatcccgt
      421 ccccgcatct ccccccggtg atcggggacc gctgcgtcgg ggacgggtgc ccggcggcgc
      481 ggttcctgac gtgagcctgt tcggattcgg tgagccggtt tactgtggag ctggaccggc
      541 cgcgggtcgg gagagcaagc gaactgccaa gtgcctgagg gctcaacgac atggcaaaaa
      601 acgggccgat ccgcacgttt tggacgcgtg atgccgggat cggacggttc cagcgcagac
      661 tgatcccatc caacgaaccg gatcttggac gccgattcgg aaaaccggat gcgggtcagg
      721 cgatttgacg aagcgggatc gagcagttat ataggggccg gacgagtttc attgaggagg
      781 attgaattca tgaatcgatt cacgcctgtc gccctgcttc tcgcgtgcgt gatggtcttt
      841 gcgatcttcg gttgctcggg tcaggggaat cagccggcgc cggaacagac ggccgagcag
      901 gcgccgccgg ccgcggagcc gccttaccag gatcaggcgg ccgcgccggt tccgccgccc
      961 gccgcgccgg ccccgtcccg atcggcgaac acgatccctg aagagcggca gccggcccgg
     1021 cccgcgccgg ccgtcccgtc gaccgtgacc atcacggtcc cggccggcac gcagctgacg
     1081 ctcgggttcc cggcggagat ctcgtccgag accgcccagc cgggggatgc cgtggtcgcc
     1141 actctgaagg aatcgatcgt catcggggat cgcgtcgcct tccccgaggg gagccaggtg
     1201 cgcggcaccg tgaccgacgt gaagccggcc tccaagggat tcaaggacac cggcggcgcc
     1261 atctcgatgc agttcaagag cgtcgtcgcg ccgaacggca ccaagtcgac catcgccgcc
     1321 ggcttcagca aggtggcgga ggggagcgcg gcgaagaagg gggccatcat cggcggcagc
     1381 gccgtcggcg gcgccctcct cggcaaggtg ctcgacaaga gcaccggcgg cggcgccgcg
     1441 gtcggcgcgg cgatcggcac cgcggtcgcc ggcaccacca aggggcgcga ggccaaaatc
     1501 aagaccgatg acatgatcgc ggtcccgctg gagcagagcg ccaaggtcgt cctgccccgc
     1561 tgagccccgc gccgcggcgc ggttctgacc cacgaaacgc cacgagcccg gcgcaccagc
     1621 ccgaattcct tcgggcctgc gtgcaccggg ctcgttgtct taacggcccg tttgcggcgg
     1681 gcggtttact tcttgttctc gaactcgttc aggcggacgt ccaccgggag ggagacgatc
     1741 accggcgccc ccgaggcgtc cgtcgccatc acgttcatca tgaagtgggc gttggcgagt
     1801 gcgttgccgc tcacgccggc caccttcacg gagtcgaacg cgaaggtcag ctccagcgcg
     1861 cccggaagac ggcgaccgcc gaccaggtga ccgctgcagc ccgccgggct gaaggcctcc
     1921 gattgcagcc cgaagctgag caccgggccg caggccccgt tgcaggagta attgacggtg
     1981 aagctcacgg gcgtccccgg ggtgacgctc ttgagcttgt aatcgcagtc ccagctgaca
     2041 ccgctcgctt cgtcggtgca ggacttctct ttcaccgcct gggcgtgcgc cggggtcgcc
     2101 agcatcgccg acaccaggaa cgccgcagcc ccgaacatga gcatcgtctt attcattgcc
     2161 gcttcacctc cggggcggcc cgaacttcgt cgcccgcgcc ctctacagag caagcccggt
     2221 accacgtcag ggggttggag gacgattccg tgatcggccg taagtcacgt cgtcgccgtg
     2281 ctcgtgcggc cggcgcggcg cggcgcgcgt gcggatcatg gacagagtac ggaagccgtt
     2341 gcgacgcgct gcgtcggcgg aaaccgacac ggtaggtcgg tcgcgacacc ggacgtcagt
     2401 caggagcgat cgtttttgga ctcgggcgcg agaaaccgct ggaattccgg ccgctgacgc
     2461 aggatcgtga acgccggatc ggtcgccgcc tgctccgccg gcatgtagcc gccgtcgcgc
     2521 gagcgtccga gaaatgacag gctcgcctcg gcgtcccgcg ccagcccgta ggcacgcgcc
     2581 atgcgatagc aggcggcggc gggtgctttg gcgtccacga ggcgcgcggc cgcgtccagg
     2641 gcccgccggg cggacgagcc ggaaggaatg gcgcgccgcc cctcggacga tcgcagccgc
     2701 gccagaagca gcgccgaatc cgtgatctcg agccggtcct cccccggatc ggcgccgagc
     2761 agggcccgct ccagaagggt ggccgcctcc gaatccttgc cgcgctcgtg gagcaggggg
     2821 gccagccgcc gcgccatgcg cgtcggggcc aggggtcggg ctgccgcctc gcgcaggagc
     2881 gccgtcacgc gatcctgccg cccgcggccg gagtaccagt cggcgagccc gtcgtaggcc
     2941 aggaacagca tctcgcggcc gcgcgccggg ccgtcgtact cggtcgggac ccgttccacc
     3001 acgaggctca ggaagtactc cgcctccgcc ccgcgatcga gcttgaggag cgcgtacccc
     3061 ttgccgtaga gactgaggag ggtcggcagc gcggcgttct ccgggagatc gacctcctgg
     3121 tacagatcga gggccccctc gtagtcgccg gcgacgacgc gcaccgcggc cgccccctcg
     3181 agggcgcgga gacggacgtc cgggaagcgg tcgaggacca tcctgtaggt gcgccccgcg
     3241 atggagaacg ccccgctgcg cgccagcgtc gcggctcctc cgagctgcgg cagcggagag
     3301 tcgtgcagca ggtcggctgc ctgaccgtag agcgtcatgg cggagtcgta ggcgccggtt
     3361 tccctcaacg tctccgcgag cgccatcaga acccgtgggg agtcgggggc gatctctgcg
     3421 gcgcgccggc aggcggagag ggacgcgtcc agatcgcgac gaagg
//
LOCUS       DASRIQ010000589         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3111113, whole genome shotgun sequence.
ACCESSION   DASRIQ010000589 DASRIQ010000000
VERSION     DASRIQ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3111113"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFD06_14555"
     CDS             complement(<1..414)
                     /locus_tag="VFD06_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04802.1"
                     /translation="MRALGHAAGFAAGLAAVPLLLGLLACGGSAPPPPASTVSVKRFP
                     APIFDGLSLEMTRAEATRVRPMRPSLTAGGKNLRIWVYDKPGDYTVHLTFGGRSESDR
                     LKRIDVHYGRETGGIGPFLARLEERYGTPDVKRRQA"
     gene            530..703
                     /locus_tag="VFD06_14560"
     CDS             530..703
                     /locus_tag="VFD06_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04803.1"
                     /translation="MTLLFRVRIEDELHEVRIEVAGDPGPDQWAGIEQFALSTAWLEH
                     RRLLPRGVLNALV"
     gene            complement(687..3122)
                     /locus_tag="VFD06_14565"
     CDS             complement(687..3122)
                     /locus_tag="VFD06_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04804.1"
                     /translation="MERVVLLSRFRPWINGAAVVAVFAAIAVLVLACPPDDLLFSHLA
                     AGDRILAQRSVPGSEEFSYTAYGMPWTDVPWLYQVGLAVLRWAFGLPVVGAVHVLLWL
                     GIFAWLFAAGRGAGTAGRAAIVLLAAAACGPWLRPEGEAVAAALLLAGLALLGSAVEA
                     ADARRRLVLWGALPALALAGANLNERFLLVLAATAVFAVDRLVAAVLRWARNSEEGAP
                     GFVAAADAAGSLALQSMAALVNPFGARALRLPFETALDPRGALPSLRALSDDFRPILS
                     GGLGAAVGLAAAALAIAALLLVLLRPGGGRLFEAAFLLILGVLALRVRRDLPPFLLAA
                     ALFALRRLPAWTGASARGATLARGALPAIAAGACVVGAGWVALDALRPVEWPPRTRPI
                     PAFLLQADDVPEAAARFVAGSALPGQVFHSLTVGGYLLDAWQRDRRIFADSRREPYEH
                     GVLATYVEAIDDPEAFERAVDKYQITAVLWPHRDAQAAAPLLRHLASPGRWGLAAVDG
                     SASLWVRIDAMSPAIARDAPLAPGQPLARTAPAILSGLDARPRRGPPLREAAIGEFFA
                     AAGDPAAAEMLFRRALERAPRAAPLWIRLGSSLEARGDRDGALLAYRAAVDLDRGTGQ
                     ADAALGRLALEAGGLDEAERHLDAAARAGDDRPSTLAARARLAVRRGRDAEARWLFAA
                     ALGEGPDRDILVEAARFEGGRGALEPALDLYARARARRPDDPVIAAEAATLLEAAGRF
                     AEALEIARGPAEGARSRSAVSGGASAEDRRLLEVAARVARRAGEPARAEEWEKAIGAA
                     GREPEENSATPER"
     gene            3155..3673
                     /locus_tag="VFD06_14570"
     CDS             3155..3673
                     /locus_tag="VFD06_14570"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HZN04805.1"
                     /translation="MAQLTAFSLKGWIDAHRELLKPPVGNALIFKDTEFQVMIVGGPN
                     RRNDYHLDPGEELFYQLEGDIVLKVMEDGTPRDIPIREGEMLLLPPNVPHSPQRGADT
                     VGLVLERRRAPGEEDRFRWYCDRCHAVVHEAALFVSDLGTQLKPVLETYRTDERLRTC
                     RACGAVNPPAGG"
BASE COUNT          556 a         1461 c         1339 g          391 t
ORIGIN      
        1 cgcctggcgc cgcttgacgt cgggggtgcc gtagcgctcc tcgaggcgcg cgaggaacgg
       61 accgatgccg cccgtctcgc gcccgtagtg cacgtcgatc cgcttgaggc gatccgactc
      121 ggagcggccg ccgaacgtca ggtggaccgt gtagtccccc ggcttgtcgt acacccagat
      181 gcgcaggttc tttccgccgg cggtcaagct cgggcgcatg ggtcggacgc gggtggcctc
      241 ggcgcgcgtc atctcgagcg acaggccgtc gaagatcggc gcggggaagc gcttgaccga
      301 caccgtcgag gccggagggg gcggcgcgct tccgccgcag gccaggagcc cgagcaggag
      361 cggcacggcg gcgagcccgg cagcgaaccc ggctgcgtga cccagagcac gcatggcggc
      421 gatgctacca ccaacccgct gcgctgccgc agcagcgctc gggtccaacc gctcgacgcg
      481 ccgccccgac cgcgacatcc tatcggcgct cgatgaaacg gacggaacca tgacgcttct
      541 cttcagggtg cggatcgagg acgagttgca cgaggtgcgg atcgaggtgg ccggcgatcc
      601 gggccccgat cagtgggccg ggatcgagca gttcgccctc agcacggcgt ggctcgagca
      661 ccggcgcctg ctgccgcgcg gcgtcctcaa cgctctggtg tagccgagtt ttcctccggc
      721 tcccggccgg ccgcgccgat cgccttctcc cactcctcgg cgcgcgctgg ctcaccggcc
      781 cggcgcgcca cccgcgccgc cacctccagg agccgccggt cctccgccga cgccccgccc
      841 gacacagcgc ttctcgagcg ggccccttcc gccggcccgc gagcgatctc cagggcctcg
      901 gcgaaccgcc ccgccgcctc caggagggtg gccgcctccg ccgcgatcac cggatcgtcg
      961 ggacgacggg cccgggcccg ggcatagaga tcgagcgccg gttcgagcgc accacggcct
     1021 ccctcgaagc gcgccgcctc gacgaggatg tcgcgatccg gtccttcccc cagcgccgcc
     1081 gcgaacagcc atcgggcctc ggcgtcgcgc ccccgccgca ccgccaggcg ggcccgggcc
     1141 gcgagggtcg aaggacggtc gtcgccggcg cgcgccgccg cgtcgagatg gcgctccgcc
     1201 tcgtcgagac cccccgcctc gagcgccagg cgcccgagcg ccgcgtccgc ctggcccgtg
     1261 ccgcggtcca gatccacggc ggcccgataa gcgagcagcg cgccatcgcg gtcgccgcgc
     1321 gcctcgagcg acgacccgag ccggatccac agcggcgccg cgcgcggcgc acgttcgagc
     1381 gcgcgccgga acaacatctc ggcggccgcc gggtcgccgg cggcggcgaa gaactccccg
     1441 atcgccgctt cgcgcagcgg cggcccgcgg cgcggccggg cgtcgagccc cgacaggatc
     1501 gccggcgccg tgcgcgccag cggctgcccg ggcgcgagcg gcgcatcccg ggcgatcgcg
     1561 ggggacatcg cgtcgatccg cacccagagg gaggcgctgc cgtccaccgc cgccagcccc
     1621 cagcgcccgg gcgaggccag atgccgcagg agcggcgccg ccgcctgcgc gtcccggtgc
     1681 ggccagagca cggcggtgat ctggtacttg tccaccgccc gctcgaaggc ctccggatcg
     1741 tcgatcgcct cgacgtaggt cgcgagcacg ccatgctcgt acggctcgcg ccgtgaatcg
     1801 gcgaagatgc gccggtcgcg ctgccaggcg tcgaggagat agccgcccac cgtcaggctg
     1861 tggaacacct gtccgggaag ggcggagccg gcgacgaagc gggcggcggc ctccggaacg
     1921 tcgtccgcct gcagcaggaa tgccgggatc ggacgcgtcc gcggcggcca ttccaccggc
     1981 cggagcgcgt cgagcgcgac ccaacctgcg ccgacgacgc acgcgccggc cgcgatcgca
     2041 ggaagcgcgc cacgggcgag ggtcgcgccc cgtgcgctcg cgccggtcca cgccggcagc
     2101 cggcgcaggg cgaagagcgc cgccgcgagg agaaacggcg ggaggtcgcg gcggacgcga
     2161 agcgccagca ccccgaggat caggaggaag gccgcctcga acaggcgccc gccgccgggc
     2221 cgcaggagca cgagcagcag ggcggcgatc gccagcgcgg cggcggcgag cccaaccgcc
     2281 gctcccagcc cgccgctcag gatcggccgg aagtcatcgc tcagcgcccg gagactcggc
     2341 aaggcgcccc gcgggtcgag tgctgtctcg aagggcagcc gcagggcgcg cgcgccgaag
     2401 ggattgacga gcgccgccat cgactggagc gccaggctgc ccgccgcatc cgccgcggcg
     2461 acgaaccccg gggcgccctc ctccgaattc cgcgcccagc gcagcaccgc cgccaccagc
     2521 cggtccacgg cgaacacggc ggtcgcggcg aggacgagga ggaagcgctc gttcaggttc
     2581 gcccccgcga gggcgagcgc cggcagggcg ccccacagca ccaatcgccg cctggcatcg
     2641 gccgcctcca cggcggagcc gagaagcgcc agccccgcga gcaggagcgc cgccgccacc
     2701 gcctcgcctt ccggccgcag ccagggcccg cacgcggccg ccgccaggag cacgatcgcc
     2761 gcgcgccccg ccgtcccggc cccgcgcccg gcggcgaaaa gccaagcgaa gatcccgagc
     2821 caaagcagga cgtgcaccgc gccgaccacc ggcagcccga aggcccaccg cagcactgcc
     2881 aggccgacct gatacagcca cgggacgtcg gtccacggca tcccgtacgc ggtgtaggag
     2941 aactcctcgg atccgggcac cgagcgctgc gccaggatac gatcccctgc cgccaggtgc
     3001 gagaagagca ggtcgtccgg cgggcaggcg aggacgagca ccgcgatcgc ggcgaagacc
     3061 gccaccaccg ccgcgccgtt gatccacggc cggaatcgtg ataacaaaac gactcgttcc
     3121 atcacacgag ggacattctg gggagggcgc gcgtttggcg caactgacgg ccttcagcct
     3181 caagggttgg atcgacgcgc accgggagct cctcaagccg ccggtcggca acgccctgat
     3241 cttcaaggac accgagttcc aggtgatgat cgtcggcggg ccgaaccgcc gcaacgacta
     3301 ccacctcgat cccggcgagg agctcttcta ccagctcgag ggggacatcg tgctcaaggt
     3361 gatggaggac ggcacgcccc gcgacatccc gatccgcgag ggggagatgc tgctcctgcc
     3421 gcccaacgtg ccgcactcgc cgcagcgcgg cgccgacacc gtcggcctgg tgctggagcg
     3481 ccggcgggcg cccggcgagg aggatcgctt ccgctggtac tgcgaccgct gccacgccgt
     3541 ggtgcacgag gcggccctgt tcgtcagcga cctcggcacc cagctcaagc cggtgctcga
     3601 gacctaccgg accgacgagc gcctgcggac ctgccgcgcc tgcggcgcgg tcaacccccc
     3661 cgccggcggt tgagcgggcg gacggggcgg aacgcgtgag cactccgttc cgggtcgatc
     3721 tgcacaccca catcctgccg cctcgcc
//
LOCUS       DASRIQ010000590         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3638708, whole genome shotgun sequence.
ACCESSION   DASRIQ010000590 DASRIQ010000000
VERSION     DASRIQ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3638708"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFD06_14575"
     CDS             <1..302
                     /locus_tag="VFD06_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04806.1"
                     /translation="SAMNLHFKVSRSLVIWGDQMLRRGPLRFLERLALHSPLVVWAPF
                     ASNLYHDSLWYPTIGRRRIARFRGTPWGRLFDTRYGPPGGGATTLARERVRAGHA"
     gene            295..1731
                     /locus_tag="VFD06_14580"
     CDS             295..1731
                     /locus_tag="VFD06_14580"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN04807.1"
                     /translation="MRRNLLLLLIAVVAVVSCRAPAGPRPNVLLITVDTLRPDHLGCL
                     GYLRPTSPAIDALAARGVVFRQAVTAAGRTVQSFPSILTGVNPPTHGLRYEGQSTEPI
                     AGRLTLTRALKEAGYDAFAVTQGLNVGLHRDFDVYDPDIYLDPQGKKVYVPTRNDFEA
                     TQKALQWLRGRRGRTSPFFLWLRYNAPHWPYDPPAPYAERFDPDYKGAHTFNEEAGPG
                     VERGDLIFGKTRLSDREVQHAVAHYDGEVAYADAAIAELLRGLDALGGTEKTIVVLTA
                     DHGESLGEHDYFFEHGAYLYEPTVRVPLIVVAPGRLPAGRKVETLGRTIDIVPTVLDL
                     AGVDVPIGLDGVSLVAWARGESKEPGRPAYSESGRNFYPENPRQKVPGVAGKWRMMRD
                     DRWKLLLIPDDPEPQWELYDLQADPGERRNVLAEHPTEADRLRRLLLAIVSRDPLSGD
                     RGEPALPDGLEDKLRSLGYVGGGPGPKP"
     gene            1728..>2168
                     /locus_tag="VFD06_14585"
     CDS             1728..>2168
                     /locus_tag="VFD06_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04808.1"
                     /translation="MRRRRLPPLLLPIGVAFACALPSLGPGFIHDDHRIIEQNQLVQE
                     AGRLPEILARGYWTVDERQVPNLYRPVTILSFALNHRVTGEGAFGFRLVDLLLHLLVV
                     GLVYGVARRMIPEGGPRGAAGGPGPPAAPVAAALLFAAHPVHTEA"
BASE COUNT          296 a          798 c          752 g          322 t
ORIGIN      
        1 tcagcgcgat gaacctgcac ttcaaggtga gccgctccct cgtcatctgg ggggaccaga
       61 tgctgcggcg cgggccgctc cgtttcctcg agcgcctggc gctgcactcg cctctggtgg
      121 tgtgggcgcc gttcgcgagc aatctgtacc acgactccct ctggtacccg acgatcgggc
      181 gccgccgcat cgcgcgcttc cggggcacgc cgtgggggcg gctcttcgac acccgctacg
      241 gcccccccgg gggaggagcg acgactctgg cccgggagcg cgtccgggcg gggcatgcgt
      301 agaaacctcc ttctcctcct catcgccgtc gtcgccgtcg tctcctgccg cgcgccggcc
      361 ggtccgcgcc ccaacgtcct gctgatcacc gtcgacacgc tgcgcccgga tcacctcggc
      421 tgcctcggct acctgcgtcc cacgagcccg gccatcgacg cgctcgcggc gcgcggcgtg
      481 gtcttccggc aggcggtcac cgccgccgga cgcaccgtgc agtcgttccc gtcgatcctg
      541 accggggtga acccgccgac ccacggcctg cgctacgagg ggcagagcac cgagccgatc
      601 gcggggcgcc tgaccctgac gcgcgcgctc aaggaggcgg ggtacgacgc gttcgccgtc
      661 acccaggggc tcaacgtcgg cctgcaccgg gacttcgacg tctacgaccc cgacatctac
      721 ctcgatcccc agggcaagaa ggtgtacgtc ccgacccgca acgacttcga ggccacacag
      781 aaggcgctgc agtggcttcg gggccggcgc ggccgcacgt caccgttctt cctctggctg
      841 cgttacaacg caccgcactg gccgtacgac ccgcccgccc cgtacgccga gcgcttcgat
      901 ccggactaca aaggggcgca caccttcaac gaggaggcgg ggccgggggt cgagcgggga
      961 gacctcatct tcggaaagac gcgcctttcc gatcgggagg tgcagcacgc ggtggcccac
     1021 tacgacgggg aggtcgctta cgccgacgcc gccatcgcgg agcttctgcg cggcctcgac
     1081 gcgctcggcg ggaccgagaa gacgatcgtc gtgctgaccg ccgaccacgg cgagagcctc
     1141 ggggagcacg actacttctt cgagcacggc gcctacctgt acgagccgac ggtgcgcgtg
     1201 ccgctcatcg tcgtggcccc ggggaggctc cccgcggggc ggaaggtcga gaccctcggg
     1261 cgcaccatcg acatcgttcc cacggtgctc gatctcgccg gggtcgacgt cccgataggg
     1321 ctcgatgggg tgagcctcgt ggcgtgggcg cgcggtgagt cgaaagagcc ggggcgcccc
     1381 gcctactccg agtcgggccg caacttctac ccggagaatc cgcggcagaa ggtgccgggg
     1441 gtggcgggga agtggcggat gatgcgcgac gatcggtgga agctcctgct catcccggac
     1501 gacccggagc cgcagtggga gctttacgac ctccaggccg atcccggcga gcggcgtaac
     1561 gtcctcgcgg agcatccgac cgaagcggat cgcctgagac ggctcctcct cgcgatcgtc
     1621 tcccgcgatc cgctgagcgg cgatcgcggc gagccggcgc tgccggacgg gctcgaggac
     1681 aagctgcgct cgctcggcta cgtcggcggc gggcctggtc cgaagccgtg agacggagga
     1741 ggcttccacc gctcctcctg ccgatcgggg tcgccttcgc ctgtgccctg ccgtcgctcg
     1801 ggcccggttt catccacgac gatcaccgca tcatcgagca gaaccagctg gtgcaggagg
     1861 ccggccgtct tccggagatc ctggcgcgcg ggtactggac ggtggacgag cggcaggtcc
     1921 cgaacctgta ccggccggtc accatcctct ccttcgccct caaccaccgg gtgaccgggg
     1981 agggggcgtt cgggttccgc ctcgtcgatc tgctcctgca cctcctggtc gtggggctcg
     2041 tctacggggt cgcgcgccgc atgattccgg aggggggccc tcgcggcgcc gccggcggtc
     2101 cgggcccgcc cgcggccccc gtcgccgcgg ccctcctgtt cgcggcgcac ccagtgcaca
     2161 ccgaagcg
//
LOCUS       DASRIQ010000591         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1546887, whole genome shotgun sequence.
ACCESSION   DASRIQ010000591 DASRIQ010000000
VERSION     DASRIQ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546887"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFD06_14590"
     CDS             complement(<1..635)
                     /locus_tag="VFD06_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN04809.1"
                     /translation="MERILIVDDQEELLSGLAVNFRREGYEVLTATSGEAALHLASRE
                     TPDLIVLDVMMPGMSGFDVCRELRRTGVEAPIIMLTARGEEIDRVLGLEIGADDYLTK
                     PFSLRELLARVRTRLRRRPAAAAGPVPAHYRFGDVEIDFEKLRAERQGRALELSAKEF
                     DLLSLLVRRRGEVLTRDEILKQVWGYDDPPLTRTVDTHILKLRQKIETDPA"
     gene            complement(635..>2347)
                     /locus_tag="VFD06_14595"
     CDS             complement(635..>2347)
                     /locus_tag="VFD06_14595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN04810.1"
                     /translation="DRGLTHEHVQTIHEDAHGTLWVGTDGGGLNRLEGERFAPVTGLP
                     SDVIGSFLEEPGGSLWVATGSGPCRMEDGRVACFGPREGMFDDIVYRILDDGIGSLWF
                     SCNKGVYRVAKQDLDDLAASRRGSVSPVVYGRPDGMRGVECNGGVEPAGWRARDGGIW
                     FPTIKGAVRFDPRRAAASATAPPLVIERALVDHRDVDLAQAPELPPGRGDLEIHYAAL
                     DLGDAHRVAYRYRLEGFDDHWIDAGARRVAYYTNVPPGRYRFQVTAARSDGAWSGAER
                     SLPFALRPMFRQTAAFYGLCTAAVLLGAWGIDRLRVRRMKARQGELEALVQQRTRSLV
                     EATRRLEEAQQRQADFVSGVSHALKTPLTLIRLYGETLQQEGFSEDDRRSYSEIITRE
                     SERLTRLIERVLDFSQVDRGEKRYRLEPGDLAAAVRRALDLVAPYLKPQGFEVDVAID
                     PRLPQARFDPDAVVDAVLNLVDNAAKYSGQARYVGIRLRTDGGRIVLEVEDRGLGISP
                     TDREHLFRKFHRGRDAAGKGGYGLGLFLVKHIMDAHGGAIEVESDVGRGSRFRLVFPA
                     GASP"
BASE COUNT          323 a          858 c          809 g          357 t
ORIGIN      
        1 ttcgccgggt ccgtctcgat tttctgccgc agcttgagga tgtgcgtgtc cacggtgcgg
       61 gtcagcggcg gatcgtcgta cccccacacc tgcttgagga tctcgtcgcg cgtgaggacc
      121 tcgccgcggc ggcggaccag gaggctcagg aggtcgaact ccttcgcgct cagctcgagc
      181 gcgcgcccct ggcgttccgc ccgcagcttc tcgaagtcga tctcgacgtc gccgaagcgg
      241 tagtgcgcgg ggacggggcc ggcggcggct gcggggcggc ggcggagccg cgtgcgcacc
      301 cgcgccagca gctcgcgcag gctgaacggt ttggtgaggt agtcgtcggc cccgatctcg
      361 agccccagca cccggtcgat ctcctcgccg cgggccgtga gcatgatgat cggcgcttcc
      421 actccggtgc ggcgcagctc ccggcagacg tcgaagccgc tcatccccgg catcatcacg
      481 tccaggacga tcaggtccgg tgtttcccgg gacgcgaggt ggagcgccgc ttcgccgctc
      541 gtggcggtca ggacctcgta cccctcgcgg cggaagttga ccgcgagccc gctcagcagc
      601 tcctcctggt cgtcgacgat gaggatccgc tccatcaggg gctcgcgccg gcggggaaga
      661 cgaggcggaa cctgctgccg cggccgacgt cgctctcgac ctcgatcgct ccgccgtgcg
      721 cgtccatgat gtgcttgacg aggaacaggc cgaggccgta tccccccttg ccggcggcgt
      781 cgcgcccacg gtggaacttc cggaacaggt gctcgcgatc cgtcggggag attccgaggc
      841 cgcggtcctc gacttcgagg acgatccggc ccccgtcggt gcgcaggcgg atccccacgt
      901 acctggcctg gcccgagtac ttggcggcgt tgtccaccag gttcagcacc gcgtccacca
      961 cggcgtccgg atcgaagcgc gcctggggca gccgcggatc gatcgccacg tcgacctcga
     1021 acccctgcgg cttcaggtag ggggccacaa ggtcgagggc ccgccggacg gccgcggcca
     1081 gatcgcccgg ctcgagccgg tagcgcttct cgccgcgatc gacctgcgag aagtcgagca
     1141 cgcgctcgat cagccgtgtg agccgctcgc tttcgcgcgt gatgatctcg gagtacgacc
     1201 ggcggtcgtc ctccgagaac ccttcctgct gcagcgtctc gccgtagaga cggatgagcg
     1261 tcaggggcgt cttgagcgcg tgcgacacgc cgctcacgaa gtcggcctgc cgctgctgcg
     1321 cctcctcgag gcgccgcgtc gcctcgacga ggctgcgggt gcgctgctgg accagcgcct
     1381 ccagctctcc ctgccgcgcc ttcatcctgc ggacccgcag gcggtcgatc ccccaggcgc
     1441 cgagcaggac cgccgcagtg cagaggccgt agaacgccgc cgtctgccgg aacatcggcc
     1501 gcagcgcgaa ggggagggat cgctccgcgc cggaccaggc cccgtcggat cgggcggcgg
     1561 tcacctggaa ccggtaccgg cccggcggga cgttcgtgta gtacgccacg cggcgcgcgc
     1621 ccgcgtcgat ccagtggtcg tcgaatccct ccagccggta gcggtaggcg acgcgatggg
     1681 cgtcgccgag gtcgagcgcc gcgtaatgga tctccaggtc gccccgaccc ggcggaagct
     1741 ccggcgcctg cgcgaggtcg acgtcacggt gatcgaccag cgcccgttcg atgacgagcg
     1801 gcggcgcggt ggcgctcgcg gcggcgcggc gcgggtcgaa gcggaccgct cctttgatgg
     1861 tgggaaacca gatcccgccg tcgcgcgccc gccatcccgc cggctcgacg ccgccgttgc
     1921 actcgactcc ccgcatgccg tccggccgcc cgtaaacgac cggactcacc gatccccggc
     1981 ggctcgccgc gagatcgtcc agatcctgtt tcgcgacccg gtagaccccc ttgttgcagg
     2041 agaaccagag actgccgatc ccgtcgtcga gaatccggta gacgatgtcg tcgaacatcc
     2101 cctcgcgcgg tccgaagcag gcgacgcggc catcctccat ccggcagggc ccggagccgg
     2161 tcgccaccca gagcgagccg ccgggttcct cgagaaacga tccgatcacg tcgctcggga
     2221 gtccggtcac cggcgcgaac ctctctccct cgagacggtt cagcccgccg ccatcggtgc
     2281 ccacccacag cgttccgtgc gcgtcttcgt ggatggtctg aacgtgttcg tgcgtgagcc
     2341 cccggtc
//
LOCUS       DASRIQ010000592         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2310332, whole genome shotgun sequence.
ACCESSION   DASRIQ010000592 DASRIQ010000000
VERSION     DASRIQ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310332"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VFD06_14600"
     CDS             <1..1024
                     /locus_tag="VFD06_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZN04811.1"
                     /translation="LQSEMARQTVVLSLVADLATAYLDLLDFDNRLTISRTTLEARTD
                     YLRIITARFDKGIVSALDVRQAEIQQSIASAAIPQFERQVAETENRIRILLGRFPGDV
                     PRGRSLYEAIVPPDVPAGLTSDLLIRRPDVQSAEASLIAANASIGVAEAQRFPLIALT
                     GSLGAASSDLSDITNGGAGTWSLGAALLQPLFNFGKNKRRVEIARAQTQQALYFYEGS
                     VQQAFREVEDALAAVRTSKAQVAAYELQVKAARSALELSTARYDQGVTSYLEVLVQQT
                     QAFDAELAASLARRDQLASIIRLYAALGGGWGLEATVPPPEPAATEGSLPAPGGAPAP
                     PATADR"
     gene            1120..2139
                     /locus_tag="VFD06_14605"
     CDS             1120..2139
                     /locus_tag="VFD06_14605"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZN04812.1"
                     /translation="MTIRGTRSGPAVLFLLLFAGLPGLLVTPVRAQTPAVAPGAEPVE
                     TEKQKRFKNTTELSLVVTAGNSDVETIGFKDTLEYKRDAGLARFRADTLRSDTSDDAY
                     LLVEPGLVFEPGQTLTTFNTREVRPGAEPDVARYFFEGRYEGNLSRQGTTWNAGASWD
                     RNEDAGIISRTIVFGGLGRAWVDREDLKFRTTYGLSYTDRVEEVVDPEKEEQFIGARF
                     TSDFMDKWGANTTYDNDFTFNMSVADTSDWNADLIQGVAVSMSNHLKLKISLQLTWAN
                     EPALEEVDIIVRAILIDPNGIPGDGDEEFHTVESGGSEITIGEGTIRKEQLDTTVRMS
                     LQIDF"
     gene            complement(2136..2516)
                     /locus_tag="VFD06_14610"
     CDS             complement(2136..2516)
                     /locus_tag="VFD06_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04813.1"
                     /translation="MSRAAASILAFGAYLALLGAGLLASPNLMLALFGQPPTAEPWLR
                     VLGLVSLVLGLYYISAARGEVTPFFRSTIWGRGIGAVVLAALVVAKTAPAFVLLMAVL
                     DGLGALWTWRALGAPPVGGPRAGS"
     gene            complement(2560..>2815)
                     /locus_tag="VFD06_14615"
     CDS             complement(2560..>2815)
                     /locus_tag="VFD06_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04814.1"
                     /translation="ARGGAILVGPKGMGPAGRYAVIRDPAGAVAALIQPAPAPDGPAE
                     RGGRKRRTARGRTRPAARRRPAVGRRSVAPRRTRRRGRRR"
BASE COUNT          441 a         1049 c          946 g          379 t
ORIGIN      
        1 tctgcagtcg gagatggcgc gtcagacggt ggtgctgtcg ctcgtcgccg acctcgccac
       61 cgcctacctc gatctgctcg acttcgacaa ccgcctgacc atttctcgca cgacgctcga
      121 ggcccgcacc gattacctgc gcatcatcac cgcccgcttc gacaagggga tcgtttcggc
      181 cctcgacgtg cgccaggcgg agatccagca gtcgatcgcc tccgcggcca tcccgcagtt
      241 cgagcggcag gtcgccgaga ccgagaatcg catccgcatc ctgctcggac gcttccccgg
      301 ggacgtgccg cgcggccgat cgctctacga ggcgattgtc ccgccggacg tcccggcggg
      361 gctcacctcc gacctgctca tccgccgccc cgacgtgcag tcggccgagg cgtcgctcat
      421 cgccgccaac gcgagcatcg gcgtcgccga ggcgcagcgc ttcccgctca tcgcgctcac
      481 cggctcgctc ggcgcggcgt cgagcgatct ctccgacatc accaatggag gcgccggcac
      541 ctggagcctc ggcgcggccc tcctgcagcc cctcttcaac ttcgggaaga acaagcggcg
      601 cgtcgagatc gcccgggcgc agacccagca ggcgctctac ttctacgagg gatcggtgca
      661 gcaggcgttc cgcgaggtcg aggacgcact ggcggcagtg cgcacgtcga aggctcaggt
      721 ggccgcctat gaactccagg tcaaggcggc ccgctcggcg ctggagctct cgacggcgcg
      781 ctacgaccag ggggtgacct cctacctcga ggtcctggtc cagcagacgc aggcgttcga
      841 cgcggagctg gccgcgtcgc tggcgcggcg ggatcagctc gcgtcgatca tccggctcta
      901 cgccgcgctc ggcggcggct ggggactcga agccaccgta ccgccgcccg agccggccgc
      961 aacggagggc tcgctccccg cgccgggcgg cgcacccgcg cccccggcga cagcggaccg
     1021 atgaccgatt gaggagcgcg tcccgcgtga aggcgactcc cgccccctct ccgcggcttc
     1081 tcgtatgatc cgcgcgcttc caatcgctga ggggcgacca tgaccattcg tggcacacgc
     1141 agcggccctg cggtcctgtt tctgctgctg ttcgcaggcc ttcccgggct cctggtcacc
     1201 cccgtccgtg cgcagactcc cgcggtcgct ccgggcgccg agccggtgga gacggaaaag
     1261 cagaagcgct tcaagaacac gacggagctg agcctcgtcg tgacggcggg gaactcggac
     1321 gtcgagacga tcggcttcaa ggacaccctc gaatacaagc gggatgccgg cctggcccgc
     1381 ttccgcgccg acacgctgcg ttccgacacg agcgacgatg cgtatctcct ggtcgagccc
     1441 gggctcgtgt tcgagcccgg ccagacgctc accaccttca acacccggga ggtgcggccc
     1501 ggggcggagc cggatgtcgc gcgctatttc ttcgaggggc gctacgaagg gaacctctcc
     1561 cggcagggga cgacctggaa cgccggcgct tcctgggatc gcaacgagga tgccggcatc
     1621 atcagcagga cgatcgtgtt cggcgggctc ggccgcgcct gggtggaccg cgaggacctg
     1681 aagttccgca ccacctacgg cctctcctac accgaccgtg tcgaggaagt cgtggacccc
     1741 gaaaaggagg agcagttcat cggcgcccgc ttcacctccg acttcatgga caagtggggg
     1801 gcgaacacca cctacgacaa cgacttcacc ttcaacatga gcgtcgcgga cacttccgac
     1861 tggaacgccg acctgattca gggcgtggcg gtgtcgatgt ccaaccacct caagctcaag
     1921 atcagcctgc agctcacctg ggccaacgag ccggcgctcg aggaggtgga catcatcgtc
     1981 cgggccatcc tcatcgatcc gaacgggatc cccggggacg gcgatgagga attccacacg
     2041 gtcgagtccg gagggagcga gatcaccatc ggcgagggga cgatccgcaa ggagcagctc
     2101 gacacgaccg tccggatgtc gctgcagatc gacttctagg agccggccct cgggcccccg
     2161 accggcggcg cgccgagcgc gcgccaggtc cacagcgccc cgagcccgtc gagcaccgcc
     2221 atcagcagaa cgaacgccgg cgccgtcttc gcgacgacca gcgccgccag cacgaccgcg
     2281 ccgatgccgc gcccccagat cgtcgagcgg aagaacggcg tcacctcccc gcgcgctgcg
     2341 ctgatgtagt acaggccgag gacgagcgac acgaggccga gcacccgcag ccacggctcg
     2401 gcggtcggcg gctgcccgaa aagggcgagc atgagattcg gcgacgcgag cagccccgcg
     2461 cccaggagcg cgagatacgc gccgaaggcg agaatgctgg ccgcggcgcg gctcacgccg
     2521 aacgctccga cgggcgggcc atgtgtgccc cgcgtcccct cagcgccttc gtccgcggcg
     2581 ccgcgtgcgg cgcggcgcga cgctccggcg tcccacggcg ggcctgcggc gtgccgccgg
     2641 gcgggtccgc ccccgggcgg tccgccgctt ccggccaccc cgctccgccg gcccgtcggg
     2701 ggcgggggcc ggctggatga gcgccgccac ggcgccggcc ggatcgcgga tgacggcata
     2761 gcgccccgcc gggcccatcc ccttcggtcc gacgaggatc gcgccgccgc gcgcc
//
LOCUS       DASRIQ010000593        15281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_476564, whole genome shotgun sequence.
ACCESSION   DASRIQ010000593 DASRIQ010000000
VERSION     DASRIQ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 15281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15281
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476564"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFD06_14620"
     CDS             complement(<1..235)
                     /locus_tag="VFD06_14620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZN04815.1"
                     /translation="MPLYEYRCRTCSRRFETLVFGSEKPSCPKCRGKDLEKLWSAFAV
                     AGGERKSESDDFGGSGSDFGGDDFGGDGGGGDMG"
     gene            complement(276..1214)
                     /locus_tag="VFD06_14625"
     CDS             complement(276..1214)
                     /locus_tag="VFD06_14625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN04816.1"
                     /translation="MQASVVINTYNKGGTLARVLEALERQEGVAPESFEVVVRDDGSS
                     DDTWSRLQGMVPRWGGRLVCSRGENTGVSEARNIGVREARGEIVIILADDIVAGPRLV
                     AEHLRRQEMERPDGPCAVVGRVLWPPELDHDPFRHWLDHGGPQFAYWRIKDTVIGPRF
                     FYACNIAARRDLFLAHPFDPAIRYGYEDTELGLRLQRAGVRLLYHPAAWGHHHHPRSI
                     EEFRQRQYRVGRSLYAALRNHPEMAAEVPPPVFPLRRRMRLALRWLTYPAARLAGPRV
                     PLALHVQQRYWRTSLHRALVRGFHDARREDPNPPRW"
     gene            1272..1976
                     /locus_tag="VFD06_14630"
     CDS             1272..1976
                     /locus_tag="VFD06_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN04817.1"
                     /translation="MRPPLLPDPLRRAMGSLRDEIRIALRAAGGKRRLRALGSRSDLR
                     LHLGCGGELKPGWVNVDLDGRGLRKALRRAPAGTIFVPYDLRRGSVPLPDGCCAVVYS
                     SHFLEHLDYEPGVRLMRDCHRLLAPGGIFRAALPTFRAMFRAYLDGDAAYFDEVPLAS
                     VRPDVEPGTETLVDHLNYGLYQYGEHRCVYDEEKLCRILERIGFRQARPSEFRPDLDP
                     DNPLRRRYSFYVEAVK"
     gene            1973..2890
                     /locus_tag="VFD06_14635"
     CDS             1973..2890
                     /locus_tag="VFD06_14635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN04818.1"
                     /translation="MKPGAPAAYAHPGYAASLSEFGTPRALPGSGGFLLERAIPGTSD
                     RDAMGCYPLFSCLDWSRLGDDLAALEGTLVSVAAVVDPFGLDDEERLRACFRDRVVPF
                     KEHFIADLDRHDLASLSRHHRYYARRALARSDVEVCVDPPQHLDEWLALYATLCARHD
                     LRGIQAFSRTAFEQQLRVPGIVLLRARSDGETIGAHLWYVQGEVAYSHLAAASPRGYE
                     AMVFYALYWRACEHFRGRVRVVDFGAGAGLAAGADDGLTRFKRGWANGTRMAYFCGRI
                     LDRERYAKILAGGRAEERYFPAYRAGEFA"
     gene            2907..4052
                     /locus_tag="VFD06_14640"
     CDS             2907..4052
                     /locus_tag="VFD06_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012684337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZN04819.1"
                     /translation="MKDLRTERLARLLVEHSCALKEGEKVVVEAIGAPVEPVLEIVRE
                     ARRRGASPLVWLKDDRIIREIAAGCGDDDVRFMADSELHALRQAQAMISLRAVHNAHE
                     YGDVPKERFGRVLRGYVGPVHLDYRNRHLRWVALRWPTPAQAQRAAMSTEAFEDFFFE
                     VCLVDYARMEREMEPLVSLLRDTDRVRIAGPGETDLRFSIRGIGARPSAGRHNLPDGE
                     VFTAPVRDSVEGRIAFNVRSTFYGTTFSNVRLAFRRGRIVEAASDQARRLEEILDQDE
                     GARFLGEFALGLNPVIRRPIDDLLFDEKMTGSLHVAAGNAYDVCDNGNRSAIHWDLIL
                     MQSAEGGGGELYFDDRLVRKDGLFVLPGLEGLNPRAADSRQARQEEV"
     gene            4056..4736
                     /locus_tag="VFD06_14645"
     CDS             4056..4736
                     /locus_tag="VFD06_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HZN04820.1"
                     /translation="MANVVVFGTSQWAELAHFYFTHDSEHDVAGFTLDREYLKEPRFK
                     GLPVVPFEEVERHFPPADFKMFIPMSFKRMNHVRAEKYAEAKRRGYALVSYVSSRATT
                     FPGFECGDNCFIFEDNTIQPFVKIGSNVVMWSGNHIGHHSVIKDHVMITSHVVISGCC
                     TIEEYCFFGVNATVRDETLIARETLVGMGCVITKDTKPFEVYKVPGTTPAEFRSDQLR
                     SLSHKSGG"
     gene            4761..5699
                     /locus_tag="VFD06_14650"
     CDS             4761..5699
                     /locus_tag="VFD06_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04821.1"
                     /translation="MRWVKKGLIFKVDGQSEWMAHHACVPIADRVSDDVLRIYFGPRD
                     GRGRTRTTFLEVEADNPSRVLRVHDRPVLDLGRLGAFDDSGVMPSCIVSHAGRKYLFY
                     IGWSPAVTVPYRNAIGLAVSDDGGLTFERLFEGPIVDRTRHEPYFTASPFALVEQGLW
                     RLWYASSTGFVVADGRPEPVYQIKYAESRDGAEWVRSGRPCIDYVRDGEANARPCVLF
                     DRGRYRMWYCFRGSRGYRTDRDESYRLGYAESPDGVVWTRMDDRVGIERSAEGWDSRM
                     MEYPFVYEHKGRKHMLYNGNGFGESGFGWAVLEDDA"
     gene            5696..6958
                     /locus_tag="VFD06_14655"
     CDS             5696..6958
                     /locus_tag="VFD06_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZN04822.1"
                     /translation="MSPLKERVEDLALFGGPPAFPSQKHVGSPNIGDRRRLLQRMEDI
                     LDRRWLTNGGPYVQEFETRVAGLLGVKHCVAMCNATVGLEIAIRALGMTGEVILPSFT
                     FVATAHALQWQRIRPVFCDVDPLTHLIDPAKIEALITPETTGILGVHLWGRPCDTQAI
                     EAIARRRGLKVLYDAAHAFGCGRGARKVGGFGDAEAFSFHATKFVNTFEGGAITTNDD
                     DLAARLRLMKNFGFVGYDDVGYIGTNGKMTEIAAAMGLTSLESLSQFVAVNRRNHRLY
                     RESLAGLPGIRLLDFDDVADHNFQYVVLEVDGGKAALHRDELAQVLWSENIVARRYFF
                     PGCHRMEPYRSLDPGAAERLPATTEVAARVLSLPTNTGVDPHDIEVIGRVVRMALSQG
                     ERIRRRLAETTPKTPIVVPHRKIEVDLA"
     gene            6990..7838
                     /locus_tag="VFD06_14660"
     CDS             6990..7838
                     /locus_tag="VFD06_14660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZN04823.1"
                     /translation="MLKPLRSFLRRRDRSRQRLVRLREEIETLRRQVDALKSSLEVPQ
                     EMIDDFFTWKERHPVPERPLVSVCVCTWNRAELLTERCIPSVLGQTWDRLELIVVGDG
                     CTDDTEERVARIRDPRLTFVNQKERGAYPRDPERRWMVAGTHAINLARRMAQGDYVTQ
                     LDDDDEYAPDRLEKLVRFAAEQGIDFVWHPFWVEGADGTWGINEAKEFASGQLTNGSV
                     FYRSWFTRVEADVTAHRLLEPGDWHRYRRIRYINPVMGRHPEPLLRHYREAVHRPVSA
                     APASRR"
     gene            complement(7741..10107)
                     /locus_tag="VFD06_14665"
     CDS             complement(7741..10107)
                     /locus_tag="VFD06_14665"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN04824.1"
                     /translation="MNAPGSSRLEALFRALVARRRAIIALYAVLIPLAAAAAARIPSD
                     SSIDRLVVQSDADFRAREEFRRLFPERAQVILLAQAPDPFAPEVLRRAAEIEAALRQV
                     PRVSPFSAPTVFEQARPGGAAADRPEEFRAFATGTDLLRRQGLVGPGFLGIPVTLDVE
                     SPADRDRVLGKIDRALAPFLSPPLTGLGKVGGPYVDAWLEGETARAGRTAFPLLGAFV
                     VVITLSLYRSVRTLLAFLLTLAAAVACALGLGHLAGYAFTIVSSLVPLTILITTTAAL
                     VYLQSRFIEIPEGRSIEDHQAFALANKFLATTASMFAAAIGFAALAVSKIRPIREMGL
                     WVGAGILVTWVVVFTLFPALQSLLRTPVRRAAAGADGGWQRLFDRLPLWTWRFRWPLL
                     AGSFLLMVAGTVALLGLPGRIAPLPLQTDALDYVDPRLPIVRDTRLFERAISGLSQVE
                     VWVTTPEGRALDPEILRGLDAFSRRLEADDRIGTVTGPITTLRFLRYAAGGGDRLPAE
                     PSAWPGLAASLDQLLLNEPALRDQIDINSLSHLRLTVLYQVKGFAGVEATRAFFGAVW
                     EAAAAEVPALREGRMRIVGQGLLQAKIAEYLVPTLTESFLLTAAIIFLAFLVVFRSGA
                     ARLMAMIPSVFAILAMFPVMRACGIPLNVATILIASTVLGASENDQIHFFYHLQEGRR
                     GAGLEAAMHHALRIAGRAILFATLINAGGFLALSFSGLPPMRQFGILASSAFVLSMLA
                     SLTALPAALWVVSGERPTGAMPAPRRPVGAPPRGSGAEAAPGGAPSPG"
     gene            10167..11015
                     /locus_tag="VFD06_14670"
     CDS             10167..11015
                     /locus_tag="VFD06_14670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN04825.1"
                     /translation="MQTRLGTASERPREPALAAPLPADRLRTCAAPERAGTFAAVYDE
                     VLTGILRDHREGKRSLLLGANDKIARFVRGTIGRGRRVLEIGCGFGRTALEVGRGQSE
                     MFGVDASQVGIDAARQAAAGRPECGFAVMDAGDLRFPDGRFDAAYSIDVVEHLHPDDV
                     GRHLAEVHRVLRSGGIYIVKTPSDLTGPHEGSDPGTQGFLHFQEYRYGTFMPLLRAAG
                     FSRLRSPAFSMRITCRLPGPPRLPSAANLLPESLALMVPYRSRACRRIARVLGVKQVV
                     IAATKS"
     gene            complement(11059..12813)
                     /locus_tag="VFD06_14675"
     CDS             complement(11059..12813)
                     /locus_tag="VFD06_14675"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HZN04826.1"
                     /translation="MPNNRAVESWVAECERLTTPDRVVWCDGSAEERDRLTRIALEVG
                     DFLPMNKERLPGCSLHRSAVNDVARVEKLTFICSGKKEDAGPINNWMAPKEAYEKLGA
                     VFKGSMKGRTMYVVPFLMGPKGSPFSKVGVEVTDSVYVALNMGLMTRMGSIALEHLGG
                     SDQFTKGLHSKADLNENRRFICHFPEDNTIWSVGSGYGGNALLSKKCMALRLASVLGS
                     KEGWLAEHMLIVGIQDPQGKTTYVCGAFPSACGKTNLAMLVPPDRFKGWKVFTVGDDI
                     AWLRPGPDGRLYAVNPEAGFFGVAPGTSRSSNPNALAMLQKDAIFTNVALKEDGTYWW
                     EGLEAPPARCTDWKGQPWTPASKEPAAHPNARFTVGIRQAPSFTEEWNNSNGVPISAI
                     LFGARRANVVPLVYEAVSWQHGVYLGATMASETTAAATGAVGVVRRDPFAMLPFCGYN
                     MADYFRHWLTVGKGLANPPKIFRVNWFNRDDQGKFIWPGFGENLRVLQWVIGRCEEKA
                     KAVATPIGLMPTPDAIDTEGLNLPSGAMDRLLEVDHEGWARALRGQEEFFDQFGGRLP
                     LELRQENEALRQRLKSAR"
     gene            12917..14062
                     /locus_tag="VFD06_14680"
     CDS             12917..14062
                     /locus_tag="VFD06_14680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN04827.1"
                     /translation="MNRWVKRLLVVAVLVACGVAVKLTLFRPKPVPVTVFRAARGRVE
                     ETVANSKAGTVTARRRAKISPAIGGQVAYLGARKGTTVKAGDVLLRIADQDLRANLDL
                     ARGEVRTAEATMREACATAAQAERDLTRSESLRGEKIVSEEIIERLRSVRDAAIARCE
                     AARAQAERGKAAVALARANLEKAVLRAPFDGIVADLKAEVGEWVSPSPPALPIPPVYD
                     IIDPTSIYVSAPIDEVDAGRVRHSLPARVTLDPLPGKSFPGKVVRVAPYVQDLEAQNR
                     TLEIEVEFDDDAFSRTLLPGTSADVEVILAAVDNVLRIPSYAILEGGKVLVLEDGALK
                     SKPVEVGMRNWEFAEVKSGLKDGESVVVSLDRAEVKEGARAVEQPAT"
     gene            14062..14739
                     /locus_tag="VFD06_14685"
     CDS             14062..14739
                     /locus_tag="VFD06_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN04828.1"
                     /translation="MIALRGMTRHYQVGDQIVRALDGVDLDVPDGDYVAIMGPSGSGK
                     STLLHLLGCLDRPTAGSYRLDGSEVGTLDDAALADVRSRKIGFVFQFFHLVPRLDASA
                     NVALPMLLAGVRPAERRERARAALAAVGLTDRADHRPDQLSGGQRQRVAIARATVMKP
                     SIVLADEPTGNLDSVSGGEILALLEGMNREGLTLLVVTHDPKVGERARRVVQMVDGRI
                     TSDGPPA"
     gene            14765..>15281
                     /locus_tag="VFD06_14690"
     CDS             14765..>15281
                     /locus_tag="VFD06_14690"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04829.1"
                     /translation="MTLVERARFAYGAVRGHALRSGLTLLGFAIGVAAVVLLTALGEG
                     ARGYVTDQFMSLGSNLIVVLPGRTETTGNAPIFGGTPRDLTIRDMQAVRQHVPRIRRI
                     APLAVGSAPAAFGDRRRESTIVGTTAEYAPMRRVAVTVGQWLPEIDPDREEPVAVLGQ
                     TVRRELFGEENP"
BASE COUNT         2313 a         5277 c         5472 g         2219 t
ORIGIN      
        1 cgcccatgtc gccgcccccg ccgtccccgc cgaaatcgtc tccgccgaag tcggagccgg
       61 agccgccgaa gtcgtccgac tccgacttgc gctcacctcc ggcgaccgcg aaggcggacc
      121 agagcttctc gagatccttc ccacggcact tcgggcagga cggcttctcg cttccgaaca
      181 cgagggtctc gaagcggcgg gaacaggtgc gacagcggta ctcgtagagc ggcatgaagg
      241 cgacctccga aatcgatccg ggcgaggagg ggagtctacc acctcggcgg attcggatcc
      301 tccctccggg catcgtggaa gccgcgcacg agcgcgcggt gcaggctcgt gcgccagtag
      361 cgctgttgca cgtggagcgc cagcggcacg cgcggccccg ccagacgggc ggcggggtag
      421 gtcagccagc gaagcgccag gcgcatgcgg cggcgcaggg ggaagacggg aggcggcacc
      481 tcggccgcca tctccgggtg gttgcggagg gcggcgtaga gggaccggcc gacgcgatac
      541 tggcgctggc ggaactcctc gatgcttcgc ggatggtggt ggtggcccca tgccgccggg
      601 tggtagagga gccgcacccc ggcccgttgc agccgcaggc cgagctcggt gtcctcgtag
      661 ccgtagcgga tggccgggtc gaaggggtgc gccaggaaga ggtcgcgccg cgccgcgatg
      721 ttgcaggcat agaagaagcg cggaccgatg accgtgtcct tgatgcgcca gtacgcgaat
      781 tgcgggccgc cgtggtcgag ccagtgtcgg aacggatcgt ggtccagctc ggggggccag
      841 aggacgcggc caacgacggc gcagggtccg tccggccgct ccatctcctg ccgccggagg
      901 tgctcggcga cgaggcgtgg cccggcgacg atgtcgtcgg cgaggatgat gacgatctct
      961 ccgcgcgcct cgcggacccc gatgttgcgc gcctccgaca cgccggtgtt ctcgccccgc
     1021 gagcacacca gccgtccccc ccagcgcggt accatcccct ggagtcggct ccacgtgtcg
     1081 tcgctcgagc cgtcatcgcg caccacgacc tcgaatgact ccggggcgac cccttcctgg
     1141 cgctcgagag cctcgagcac tcgggcgagg gtccctccct tgttgtaggt gttgatgacg
     1201 acgctggcct gcatcggtac gcctatctta cgggaagagg ggagggccga atcgccttac
     1261 aatccctgtc cttgcgacct ccgctcctcc ccgatccgct gcgccgcgcc atgggctccc
     1321 tgcgcgacga gatccgcatc gccctcaggg cggcgggcgg gaagcggcgc ctccgggctc
     1381 tgggctctcg atccgacctg cgcctccacc tcggctgcgg cggcgagctg aagcccggat
     1441 gggtcaacgt cgatctcgac ggccgcggtc tgcgcaaggc gctgcggcgc gccccggccg
     1501 ggacgatctt cgtcccgtac gacctgcgtc gcgggtcagt gccgctcccg gacggctgct
     1561 gcgccgtcgt ctactcctcg cattttctcg agcacctcga ctacgagccg ggggtgcgtc
     1621 tgatgcgcga ctgccaccgg ctgctggcgc ccggcgggat cttccgcgcg gcgctcccca
     1681 ccttccgcgc catgttccgt gcctacctcg acggcgacgc cgcctatttc gacgaggtgc
     1741 cgctcgcttc ggtgcggccg gacgtcgagc cggggaccga gacgctcgtc gatcacctca
     1801 actacgggct ctaccagtac ggcgagcacc ggtgcgtcta cgacgaagag aagctgtgcc
     1861 ggatcctcga gcggatcggc ttccgacagg cgcgtccgtc ggagttccgg ccggacctcg
     1921 atccggacaa cccgctgcgc cgccgctatt ccttctacgt ggaagcggtg aagtgaaacc
     1981 cggggccccc gcagcctatg cccatcccgg ctacgccgcg tcgctgagcg aattcgggac
     2041 gccgcgggcg cttccgggaa gcggtggctt cctgctcgag cgggccatcc ccgggacctc
     2101 cgatcgggac gcgatggggt gttatccgct cttctcgtgc ctcgactgga gccgcctcgg
     2161 agacgacctg gcggcgctcg aggggacgct ggtgtccgtg gcggcggtgg tcgatccgtt
     2221 cgggctcgac gacgaggaac gtctgcgcgc ctgtttccgc gatcgcgtcg tgccattcaa
     2281 ggagcatttc atcgccgatc tcgatcgtca cgacctggcg tcgctgtcgc ggcaccatcg
     2341 ctactacgca cggcgcgccc tggcccggag cgacgtcgag gtgtgcgtcg atccgccgca
     2401 gcacctcgac gagtggctcg ccctctacgc cacgctgtgc gcccggcacg atctccgggg
     2461 gatccaggcg ttctcgcgga cggcgttcga acaacagctc cgcgtgccgg ggatcgtcct
     2521 gctgcgcgcg cggagcgacg gcgagacgat cggcgcgcac ctctggtacg tgcaggggga
     2581 ggtggcgtac agccacctcg ccgccgcgag cccgcgcggc tacgaggcga tggtcttcta
     2641 cgccctctac tggcgcgcct gcgagcattt ccgggggcgc gtccgcgtcg tcgacttcgg
     2701 cgcgggggcg gggctcgcgg cgggggccga cgacggcctg acgcgcttca agcgcggctg
     2761 ggcgaacggc acgcgcatgg cctatttctg cggccggatc ctcgaccggg agcgctatgc
     2821 gaagatcctg gccggcggcc gcgcggagga gcggtacttc cccgcgtacc gggccgggga
     2881 gttcgcatga accgcggtgc cctgtcatga aggatctgag aacggagcgg ctggcgcgcc
     2941 tgctcgtcga gcactcctgc gccctgaagg aaggggagaa ggtggtcgtc gaggcgatcg
     3001 gcgcgccggt cgagccggtg ctcgagatcg tccgggaggc gcggcggcgg ggcgcctccc
     3061 ccctcgtctg gctcaaggac gatcggatca tccgggagat cgcggccggg tgcggggacg
     3121 acgatgtccg cttcatggcc gactcggagc tacacgcgct gcgccaggcg caggcgatga
     3181 tcagcctccg ggcggtccac aatgcgcacg agtacggcga cgtgcccaaa gagcgcttcg
     3241 gccgggtcct gcgcggctac gtcggaccgg tccatctcga ctaccgcaac cgccacctgc
     3301 gctgggtcgc cctgcgctgg ccgaccccgg cccaggcgca gcgggcggcg atgagcacgg
     3361 aggccttcga ggacttcttc ttcgaggtgt gcctcgtcga ctacgcccgc atggagcggg
     3421 agatggagcc gctcgtgagc ctcctccgcg acaccgaccg ggtgcggatc gcggggccgg
     3481 gggagaccga cctgcgcttc tccatccggg gcatcggggc acgcccctcc gccggccggc
     3541 acaacctgcc ggacggcgag gtcttcacgg cgccggtgcg cgattcggtc gaggggcgga
     3601 tcgccttcaa cgtccggtcc accttctacg gcaccacctt ctcgaacgtg cgcctcgcgt
     3661 tccggcgcgg gcgcatcgtc gaggccgcct ccgaccaggc gcggcggctc gaggagatcc
     3721 tcgatcagga cgagggggcg cgcttcctcg gagagttcgc cctcgggctg aacccggtga
     3781 tccgccggcc gatcgacgat ctgctgttcg atgagaaaat gaccggctcg ctgcacgtcg
     3841 cggccggcaa cgcctacgac gtgtgcgaca acggcaaccg gtcggcgatc cactgggacc
     3901 tgatcctgat gcaatcggcg gagggcgggg gcggggagct gtacttcgac gaccggctcg
     3961 tccggaagga cggcctgttc gtcctgccgg ggctcgaagg gctcaaccct cgagccgccg
     4021 attcacgaca ggcccgtcag gaggaggtct aggtcatggc gaatgtcgtc gtcttcggaa
     4081 ccagccagtg ggcggagctg gcccacttct acttcacgca cgactccgag cacgatgtgg
     4141 ccggcttcac cctcgaccgc gagtacctga aggaaccgcg gttcaagggg ctgccggtcg
     4201 tgccgttcga ggaggtggag cggcacttcc cgcctgcgga cttcaagatg ttcatcccga
     4261 tgtcgttcaa gcgcatgaac cacgtgcgcg ccgagaagta cgccgaggcg aagcggcgcg
     4321 gctacgcgct ggtgtcgtac gtcagcagcc gcgcgacgac cttccccggg ttcgagtgcg
     4381 gcgacaactg cttcatcttc gaggacaaca ccatccagcc gttcgtgaag atcggcagca
     4441 acgtcgtgat gtggagcggg aaccacatcg ggcaccacag cgtcatcaag gaccacgtga
     4501 tgatcacctc gcacgtggtg atctccggct gctgcaccat cgaggagtac tgcttcttcg
     4561 gcgtcaacgc gacggtgcgc gacgagacgc tgatcgcgcg cgagaccctc gtcggcatgg
     4621 ggtgcgtcat caccaaggac acgaagccgt tcgaggtcta caaggtcccc gggacgactc
     4681 ccgcggagtt ccgcagcgac cagctccgca gcctgtcgca caagtcgggc gggtaggcgg
     4741 cggagcgggg gacgagcggc gtgcgctggg tcaagaaggg gctcatcttc aaggtggacg
     4801 ggcagtccga gtggatggcg caccacgcct gcgtcccgat cgccgaccgg gtgagcgacg
     4861 acgtgctgcg catctacttc ggcccgcgcg acgggcgcgg acggacccgc acgaccttcc
     4921 tcgaggtcga ggcggacaat ccctcgaggg tgctgcgggt gcacgaccgg ccagtgctcg
     4981 acctcgggcg tctcggggcg ttcgacgaca gcggcgtgat gccctcatgc atcgtcagcc
     5041 acgccggccg caagtacctc ttctacatcg gctggagtcc ggcggtgacc gtgccgtacc
     5101 gcaacgccat cggcctcgcc gtcagcgacg acggcggtct caccttcgag cggctcttcg
     5161 aggggccgat cgtcgaccgg acccgccacg agccgtactt caccgcctcg ccgttcgccc
     5221 tcgtggagca aggcctctgg cggctctggt acgcctcgag caccggcttc gtcgtggcgg
     5281 acggcaggcc ggagccggtc taccagatca aatatgccga gagccgcgac ggcgccgagt
     5341 gggtccgcag cggccgcccc tgcatcgact acgtccgcga cggggaggcc aacgcgcgcc
     5401 cgtgcgtcct gttcgaccgg ggacggtacc ggatgtggta ctgcttccgc ggcagccgcg
     5461 gctaccggac cgaccgcgac gagagctacc gcctcggcta cgccgagtcg cccgacggtg
     5521 tcgtctggac ccgcatggac gaccgcgtcg ggatcgagcg ctccgccgag ggctgggact
     5581 cacggatgat ggagtacccg ttcgtctacg agcacaaggg caggaagcac atgctgtaca
     5641 acggcaacgg gttcggggaa tcggggttcg gatgggcggt gctcgaggac gacgcatgag
     5701 cccgctcaag gagcgggtcg aggacctggc gctgttcggc ggccccccgg ccttcccgtc
     5761 gcagaagcac gtcggcagcc cgaacatcgg cgaccgccgg cgcctgctgc agcggatgga
     5821 ggacatcctc gaccgccgct ggctgaccaa cggcgggccc tacgtgcagg agttcgagac
     5881 gcgggtcgcg gggcttctcg gcgtgaagca ctgcgtcgcg atgtgcaacg cgacggtcgg
     5941 cctcgagatc gccatccgcg cgctcggcat gacgggggag gtgatccttc cgtccttcac
     6001 gttcgtcgca accgcgcacg ccctgcagtg gcagcggatc cgcccggtct tctgtgatgt
     6061 cgatccgctc acccatctca tcgacccggc gaagatcgag gcgctcatca cgccggagac
     6121 gaccgggatc ctgggggtgc acctgtgggg tcggccctgc gacacgcagg cgatcgaggc
     6181 gatcgcgcgg cggcgcgggc tcaaggtcct gtacgacgcg gcgcacgcct tcgggtgcgg
     6241 gcggggggcg cggaaggtcg gcgggttcgg cgacgccgag gccttctcgt tccatgccac
     6301 caagttcgtg aacaccttcg aaggcggggc gatcacgacg aacgacgacg acctcgcggc
     6361 gcgcctccgc ctgatgaaga acttcggatt cgtcggctac gacgacgtcg ggtacatcgg
     6421 caccaacggc aagatgaccg agatcgccgc cgccatgggt ctgacctcgc tcgagagcct
     6481 gtcgcagttc gtcgcggtca accggaggaa tcacaggctc taccgcgaat cgctcgccgg
     6541 gctgccgggg atccgcctgc tcgacttcga cgacgtcgcc gatcacaact tccagtacgt
     6601 cgtcctcgag gtggacggcg ggaaggcggc cctgcaccgg gacgagctgg cgcaagtcct
     6661 gtggtcggag aacatcgtcg cccgccggta cttcttcccc ggctgccacc gcatggagcc
     6721 gtaccggagc ctcgaccccg gggcggcgga gcggctgccg gcgacgaccg aggtcgcggc
     6781 gcgcgtgctg tcgctgccga ccaacaccgg cgtcgatccg cacgatatcg aggtgatcgg
     6841 ccgcgtcgtc cgcatggcgc tgtcgcaggg ggagcggatc cggcgccgtc tggccgagac
     6901 caccccgaag accccgatcg tcgtgccgca ccggaagatc gaagtggacc tggcgtgagc
     6961 ggggtcatcc ggctccggac aggtgctcct tgctgaagcc cctgcggtcc tttctccgaa
     7021 gacgcgaccg atcgcgacag cgcctcgtgc gcctgcgcga ggagatcgag acgctccgcc
     7081 ggcaggtgga cgcgctcaag tccagtctcg aagtcccgca ggagatgatc gacgacttct
     7141 tcacctggaa ggagcgccac ccggtgcccg agcgtccgct cgtcagcgtc tgtgtctgca
     7201 cctggaaccg ggcggagctc ctgaccgagc ggtgcatccc gtcggtgctg ggacagacct
     7261 gggaccgcct cgagctcatc gtcgtggggg acggctgcac cgacgacacc gaggagcgcg
     7321 tcgcccgcat tcgcgacccg cgcctcacgt tcgtcaacca gaaggagcgg ggcgcctatc
     7381 cgcgcgaccc ggagcgccgc tggatggtcg ccggcaccca cgccatcaac ctcgcccgcc
     7441 gcatggccca aggggactac gtcacccagc tcgacgacga cgacgagtac gcgccggacc
     7501 gactcgagaa actggtgcgc ttcgccgccg aacaggggat cgacttcgtc tggcacccgt
     7561 tctgggtcga gggggcggac gggacgtggg ggatcaacga ggcgaaggag ttcgcctccg
     7621 ggcagctgac gaacgggtcg gtcttctacc gttcctggtt cacccgcgtc gaggcggacg
     7681 tcaccgccca ccggctcctc gagccggggg actggcaccg ctaccgccgc atccgctaca
     7741 tcaacccggt gatggggcgc cacccggagc cgcttctgcg ccactaccgc gaggcggtgc
     7801 accgaccggt ctccgcggcg ccggcatcgc gccggtaggc cgctctcccg aaacgaccca
     7861 cagcgcggcc gggagcgccg tcaggctggc gagcatcgac aggacgaacg cgctcgacgc
     7921 caggatgccg aattggcgca tcggcggcag ccctgagaag gagagcgcca ggaagccgcc
     7981 ggcgttgatc agcgtcgcga acaggatcgc ccgcccggcg atgcgcagcg cgtggtgcat
     8041 ggccgcttcg agccccgccc cccggcgccc ctcctgcagg tggtagaaga agtggatctg
     8101 atcgttctcc gacgccccga ggacggtgga agcgatcagg atggtggcga cattgagcgg
     8161 gatgccgcag gcgcgcatca ccgggaacat cgcgaggatg gcgaagaccg acgggatcat
     8221 cgccatcagc cgggcggcgc cgctgcggaa cacgacgaga aacgccagga agatgatcgc
     8281 cgcggtcagc aggaagctct cggtcagcgt cgggacgaga tattccgcga tcttcgcctg
     8341 cagcagcccc tgaccgacga tccgcatccg cccctcccgg agcgcgggca cctccgcggc
     8401 ggccgcctcc cagaccgctc cgaagaacgc ccgggtcgcc tccacgcctg cgaacccctt
     8461 cacctggtag aggacggtga gccggaggtg cgacagggag ttgatgtcga tctggtcgcg
     8521 cagcgccggc tcgttcagga ggagctggtc gagcgacgcg gcgagccccg gccaggcgga
     8581 cggctccgcc gggagtcggt cgccgcctcc cgcggcgtag cgcaggaaac gcagcgtcgt
     8641 gatcggcccg gtcacggtcc cgatccgatc gtccgcttcg agccggcgcg agaacgcatc
     8701 gaggccgcgg aggatctccg ggtcgagcgc ccgtccctcc ggcgtcgtca cccagacctc
     8761 cacctgtgag aggccggaga tcgcccgctc gaagaggcgc gtgtcgcgga cgatcggcag
     8821 gcgcggatcg acgtagtcga gggcatcggt ctggagcggc agcggcgcga tgcgccccgg
     8881 caggccgagg agcgcgaccg tccccgccac catcagcagg aaggatccgg cgagcaacgg
     8941 ccagcggaac cgccacgtcc acaacggcag ccggtcgaag agccgctgcc agccgccgtc
     9001 ggcgccggcc gccgcgcgcc ggaccggcgt ccggagcagg ctctgcagcg ccgggaacag
     9061 ggtgaacacc acgacccagg tcacgaggat cccggctccg acccagagcc ccatctcccg
     9121 gatcggccgg attttcgaca cggccagcgc cgcgaagccg atcgccgccg cgaacatcga
     9181 ggcggtggtg gcgaggaact tgttcgcgag ggcgaaggcc tggtggtcct cgatcgatcg
     9241 cccctccggg atctcgatga agcggctctg gagatacacg agggccgcgg tcgtggtgat
     9301 caggatggtc agcggcacga gggacgagac gatggtgaac gcgtatccgg ccaggtgtcc
     9361 gagaccgagc gcgcaggcga cggcggcggc aagcgtcagg aggaacgcca gaagcgtccg
     9421 caccgaacgg tacagcgaca gggtgatgac gacgacgaag gccccgagaa gcggaaaggc
     9481 ggtccggccg gcccgcgccg tctccccctc gagccaggca tcgacgtagg gtccccccac
     9541 cttgccgagg ccagtgaggg gcggggagag aaacggcgcc agggcccgat cgatctttcc
     9601 caggacccgg tcgcgatcgg cgggggactc gacgtcgagc gtcaccggga tgccgaggaa
     9661 gccggggccg acgagcccct gtcgccgcag cagatcggtg ccggtggcga aggcgcggaa
     9721 ctcctccggc cgatccgcgg ccgctcctcc cggacgggcc tgctcgaaga cggtcggcgc
     9781 cgagaacggg gagacgcgcg gcacctgccg cagcgccgcc tcgatctccg cagccctgcg
     9841 caggacctcg ggtgcgaacg gatcgggcgc ctgcgccagc aggatcacct gggcccgctc
     9901 cgggaagaga cgccggaact cctcccgggc gcggaagtcg gcgtcgctct ggacgacgag
     9961 ccggtcgatc gagctgtcgg aggggatgcg cgccgccgcc gcggcggcga gcgggatcag
    10021 gacggcatag agcgcgatga tggcgcggcg ccgcgcgacg agtgcgcgga acagcgcctc
    10081 gagtcgcgac gagcccggcg cgttcatggg ccgcgtaggg tatcccacgt cggtgcgccg
    10141 gttcgcggtg cgggtatact tcgcccgtgc agacccggtt gggcaccgcc tcggagcggc
    10201 cgcgcgagcc cgcgctggcc gcgccgctcc ctgccgaccg gctgaggacc tgcgccgctc
    10261 ccgagcgagc cggcacgttc gccgccgtct acgacgaggt cctgacgggg atcctgcgtg
    10321 atcatcgcga ggggaagcgg tcgctccttc tcggcgccaa cgacaagatc gcccgcttcg
    10381 tccgcgggac gatcggccgc ggccggaggg tcctcgagat cggctgcggc ttcggccgga
    10441 cggccctcga agtcggacgc ggacagagcg agatgttcgg cgtggacgcg tcgcaggtgg
    10501 ggatcgacgc cgcccgccag gcggccgcgg ggcgcccgga gtgcgggttc gccgtcatgg
    10561 acgcgggaga tctgcgcttc ccggacggcc gcttcgacgc ggcctactcg atcgacgtcg
    10621 tggagcacct gcatcccgac gacgtcgggc gccatctcgc cgaggtgcac cgcgtcctga
    10681 gatcgggcgg gatctacatc gtcaagacgc cgagtgacct gaccggtccg cacgaaggga
    10741 gcgacccggg gacccaaggg ttcctgcact ttcaggagta tcgctatgga accttcatgc
    10801 cgcttctgcg ggccgccggc ttctcccgcc tgcgttctcc ggcgttcagc atgcggatca
    10861 cctgtcgcct gccgggtccc ccccgcctgc cgtcggcggc gaacctgctc cccgaatcgc
    10921 tggcgctcat ggtgccctac cggagccggg cgtgccgacg catcgcgcgc gttctcggcg
    10981 tgaagcaggt cgtgatcgcg gcgacgaaaa gctagcccgg cgccggtgcc cgccgcgtcc
    11041 gccgcgggac ggcggagatc agcgggcgct cttcagccgc tgccgcagcg cttcgttctc
    11101 ctggcggagc tcgagaggca ggcggccgcc gaactgatcg aagaactcct cctggccgcg
    11161 cagcgcccgc gcccagccct cgtgatccac ttcgaggagc cggtccatcg cgccggaggg
    11221 aaggttgagc ccctcggtgt cgatggcgtc gggggttggc atcagaccga tcggtgtcgc
    11281 gaccgccttg gccttctcct cgcagcgccc gatcacccac tgcagcaccc ggaggttttc
    11341 gccgaacccg ggccagatga acttcccctg atcgtcccgg ttgaaccagt tgacgcggaa
    11401 gatcttcggc gggttcgcca gccctttccc gaccgtcagc cagtggcgga agtagtcggc
    11461 catgttgtag ccgcagaagg ggagcatcgc gaacggatcg cggcgcacga cgccgacggc
    11521 gccggtggcg gcagcggtgg tctcggaggc catggtggcg ccgagataga cgccgtgctg
    11581 ccacgacacg gcctcgtaca ccagcggcac gacgttggcg cggcgggccc cgaacaggat
    11641 ggcgctgatc ggcacaccgt tcgagttgtt ccactcctcg gtgaacgacg gcgcctggcg
    11701 gatcccgacg gtgaagcggg cgttcgggtg ggcggccggc tccttcgagg cgggggtcca
    11761 cggctgcccc ttccagtcgg tgcagcgcgc gggcggcgct tcgagcccct cccaccagta
    11821 ggtcccgtcc tccttgaggg cgacattggt gaagatggcg tccttctgca gcatcgccag
    11881 ggcattcggg ttggaagagc ggctggtccc cggcgcgacg ccgaagaagc cggcctcggg
    11941 gttgacggcg tacagccgcc cgtccggacc ggggcgcagc cacgcgatgt cgtcgccgac
    12001 cgtgaacacc ttccagccct tgaagcgatc ggggggcacg agcatcgcca ggttcgtctt
    12061 gccgcaggcg ctcgggaagg cgccgcagac gtaggtcgtc tttccctgcg gatcctggat
    12121 cccgacgatg agcatgtgct cggcgagcca gccctccttc gagccgagga cgctcgcgag
    12181 ccgcagcgcc atgcacttct tcgacagcag cgcattgccg ccgtagccgg acccgacgct
    12241 ccagatggtg ttgtcctcgg ggaagtggca gatgaagcgg cggttctcgt tgaggtccgc
    12301 cttggagtgg agccccttgg tgaactgatc cgatcccccg aggtgctcga gcgcgatcga
    12361 ccccatccgg gtcatcagcc ccatgttgag ggcgacgtag acgctgtcgg tcacctcgac
    12421 cccgaccttg ctgaagggcg agcccttcgg ccccatcagg aagggcacga cgtacatcgt
    12481 ccgccccttc atcgatccct tgaaaacggc gccgagtttc tcgtacgcct ccttgggcgc
    12541 catccagttg ttgatggggc cggcatcctc cttcttcccg gagcagatga aggtcagttt
    12601 ttcgacgcgc gccacgtcgt tgacggcgct gcggtgaagg gaacagccgg ggaggcgctc
    12661 cttgttcatc ggcaggaagt cgccgacttc gagcgcgatg cgggtcaggc ggtcccgctc
    12721 ttccgccgag ccgtcacacc agacgacgcg atcgggcgtc gtgaggcgtt cacactccgc
    12781 cacccaggat tcgaccgccc tgttgttcgg catgctggcg accccctgaa agcggccatt
    12841 atacggccgc tcccggcgcc cggagggccc ggccgggcgt gatactctcc accgcttccc
    12901 gggggcgggc gctcgaatga atcgctgggt caagaggctc ctggtggtcg ccgtactcgt
    12961 cgcctgcggc gtcgccgtga agctcacgct gttccgtccg aagccggtgc cggtgaccgt
    13021 gttccgggcg gcgcgcggtc gcgtcgagga aacggtcgcc aacagcaagg cggggaccgt
    13081 gactgcccgg cggcgcgcca agatcagccc ggcgatcggc gggcaggtgg cgtacctcgg
    13141 ggcccgcaag gggacgacgg tgaaggccgg cgacgtcctc ctgcgcatcg ccgaccagga
    13201 tctgcgcgcc aacctcgatc tcgcccgcgg ggaggtgcgg accgccgaag cgacgatgcg
    13261 ggaggcgtgc gccacggcgg cgcaggcgga acgcgacctg acccgcagcg agagcctgcg
    13321 cggcgaaaag atcgtctcgg aggagatcat cgaacggctg cgcagcgtgc gcgacgccgc
    13381 gatcgcccgc tgcgaggcgg cgcgggccca ggccgagcgg gggaaggcgg cggtggcgct
    13441 ggcgcgggcc aacctcgaga aggcagtcct gcgggccccc ttcgacggca tcgtcgccga
    13501 cctgaaggcg gaggtcggag agtgggtctc gccgtcgccg ccggcgctgc cgatcccgcc
    13561 tgtctacgac atcatcgatc cgacgtcgat ctacgtgagc gccccgatcg acgaggtgga
    13621 cgccgggcgc gtccgccact cgctgccggc gcgcgtcacc ctcgaccccc tcccggggaa
    13681 gtcgttcccg gggaaggtgg tccgggtcgc gccgtacgtg caggatctgg aggcgcagaa
    13741 ccgcactctc gagatcgaag tggagttcga cgacgacgcc ttcagccgca cgctcctccc
    13801 cggcacctcc gccgacgtcg aggtgatcct cgcggccgtc gacaatgtgc tgcgcatccc
    13861 ctcgtacgcg atcctcgagg ggggcaaggt gctggtgctg gaggacggcg ccttgaagtc
    13921 gaagccggtc gaggtcggca tgcggaactg ggagttcgcc gaggtcaaaa gcggcctgaa
    13981 ggacggggag tcggtggtcg tgtcgctcga tcgcgcggag gtgaaggagg gggcgcgcgc
    14041 cgtcgagcag ccggcgacct gatgatcgcg ctgcgcggca tgacccggca ctaccaggtc
    14101 ggcgaccaga tcgtccgcgc cctcgacggt gtcgacctcg acgtgccgga cggcgactac
    14161 gtcgccatca tgggtccctc cggctcgggg aagtcgaccc tcctgcacct cctcggatgc
    14221 ctcgatcggc cgaccgcggg ctcgtaccgc ctcgacggga gcgaggtggg gacgctcgac
    14281 gacgcggcgc tcgccgacgt ccgatcgcgc aaaataggct tcgtgttcca gttcttccac
    14341 ctcgtgccgc ggctcgacgc gtcggccaac gtcgcgctgc cgatgctgct cgcgggcgtg
    14401 cggcccgcgg agcgccgcga gcgtgcgcgg gcggccctcg ccgcggtcgg gctgaccgac
    14461 cgggccgacc accgcccgga tcagctgtcc ggcgggcagc ggcagcgggt ggcgatcgcc
    14521 cgcgccacgg tgatgaagcc ctcgatcgtc ctcgccgacg aaccgaccgg gaacctcgac
    14581 agcgtctcgg gcggggagat tctggcgctg ctcgaaggga tgaaccgcga ggggctgacg
    14641 ctcctcgtcg tgacccacga tcccaaggtg ggggagcggg cccggcgcgt cgtgcagatg
    14701 gtggacggca ggatcaccag cgacgggccc ccggcatgag cggctccgcg ccggaaggtc
    14761 cgcgatgacc ctggtcgagc gggcgcgctt cgcgtacggg gcggtgcgcg gccacgcgct
    14821 gcgatccggg ctgacgctcc tcgggttcgc catcggcgtg gcggcggtcg tgctgctcac
    14881 cgccctcggg gagggggcgc gcggctacgt caccgatcag ttcatgtcgc tcggcagcaa
    14941 cctgatcgtc gtcctgccgg ggaggaccga gacgaccggc aacgcgccga tcttcggggg
    15001 cacgccgcgc gatctgacca tccgcgacat gcaggcggtg cggcagcacg tgccgcgcat
    15061 ccggaggatc gcgccgctcg ccgtcgggtc ggccccggcg gcgttcggcg accggcgccg
    15121 cgagtccacc atcgtcggca cgaccgccga gtacgccccg atgcggcgcg tggcggtgac
    15181 cgtggggcag tggctcccgg agatcgatcc ggatcgggag gagccggtgg cggtcctggg
    15241 acagacggtg cggcgcgagc tgttcgggga ggagaacccg a
//
LOCUS       DASRIQ010000594         5606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2572789, whole genome shotgun sequence.
ACCESSION   DASRIQ010000594 DASRIQ010000000
VERSION     DASRIQ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572789"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2052)
                     /locus_tag="VFD06_14695"
     CDS             complement(<1..2052)
                     /locus_tag="VFD06_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04830.1"
                     /translation="MNALFAPGAKRNRMIVAFGLSLLLATPAIVLWADKTQRSDIEGE
                     DAYELKHAQDEARRLSSIVLPEGTLPPAEFSPFGGVLKPRRLQGGAGDDLLFSPIGDR
                     SWNPNDDALLQSVKPGLRWGQSELTQGQGFSVRPGLNYVRLSQSAIDQQGLDAVMASV
                     SEIGTVLSVLPRRTLLVNVEARNMHLLRQSSAIDQATAMQPADKLAADLGARPYIEKR
                     RASDPKLQVRVALIPGVSTTDMAKKVAATPGVSEVTENETGGELHLKVHYGSIDKLAR
                     IDEIVTVDEIREMVLFNDENVPTIQAGSAEDANLIRPFDVAGVDGGGIDSNADGERVN
                     DGVDPVRPQIVTVTDNGISIDTPNFSQTATQVTTVIPARPIGPQHRKIHAIQNVVDSG
                     TSCDAQLSGAGTHGHIVASAIAAYPSQFGIGASRGNNGARPTQPRNENMDGVARGARI
                     ILQDVGTVAQCTINSLVERGGNVSPGSLTDRLNGALTGAGADGHLAVMPFGAPSNFST
                     IQFLASNGTYPAEAVQVDTFLYNNRDYMVFVPVGNNGGLVGTNRLGLALRVIPDLFNG
                     TALDENSNIPQDIQIPPPSTAKNITSVGSSTDDCFTFFGSTDCEQTINNFTSRGPATQ
                     ESLRMAPLVMAPAFDLIGTPMTAGVAVLRSNDNDNLGPVDAQLDEGNFGSSFSAAYMT
                     GA"
     gene            2511..3029
                     /locus_tag="VFD06_14700"
     CDS             2511..3029
                     /locus_tag="VFD06_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZN04831.1"
                     /translation="MALLTAAGAKVAVPLPGTPVPATLQTLPVLLAGLLLGSRIGALS
                     QVVYLTLGLAGLPVFALPGAGPAYLLGPTGGYLLGFVAAAWITGALAPPSPGRGLLRR
                     FAAVIVGIAAIHAAGVLWLALLFAPDTFDAFRVGSLPFIAFDLAKGILACAAAAGATF
                     ARSALWTSRSTD"
     gene            3059..3796
                     /gene="larE"
                     /locus_tag="VFD06_14705"
     CDS             3059..3796
                     /gene="larE"
                     /locus_tag="VFD06_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013321137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HZN04832.1"
                     /translation="MVAFSGGVDSTLLLKVAHDVLGPRVTAVTAVSASLAADERRETE
                     RLAAGIGVAHRFVETLEMGDPDYVANRGRRCYHCKRELFRVLRAIASGAGHDVTLLYG
                     AIVDDLGDERPGMQAAAEARVRAPLLEAGFTKEMTRDLSRSLGLPNWDKPAMACLASR
                     LPIGTPVTTEALQRIDRAEAAVRRLGYRHVRVRAAGDSARLELDPADLRRALLAFERE
                     RLISVIVAAGFETVEVDPRGYRPGGRG"
     gene            3984..5480
                     /locus_tag="VFD06_14710"
     CDS             3984..5480
                     /locus_tag="VFD06_14710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZN04833.1"
                     /translation="MSLADLLRRRQWPVPFRPEPVPPVALLDRLIAAANPLPMRPGAG
                     CAASIVRAGAPRQAAAAALAEAGVDATELALLEPAAVLCTVASPDSDGAAACHLGAGQ
                     IALAAEEAGLGALVLPLPRPIRIEDGPAGQVFAVVGIGGSPVRPPGPAPAGARAEQPG
                     SPAVLLSLLEIAAATNAAEDLDRLLESIAQELARLFPVDRVDAAFAEEGVIQAAVIAP
                     RDPSGRRAAVDRLPVDDTHHLGAVVSQGRALWRNDLTMELRFRETLPRGGMRSDMTIP
                     LRSRGQATGALRLASRRPHAYEAEDFEILERLADVMAMAVENQRLLQVTRRMAEVDGL
                     TGVCNRRHFQVLLAREAERARSAGHPLGLMLADVDRFKSVNDRFGHPAGDAVLQHVAR
                     VLGRRLRRSDVVARYGGEEFAVLLPGTDRDAAASLAEELRREVESAPATLPAPAAPLA
                     VTVSLGVASMPADALDENGLVTAADRALYRAKEGGRNRVERAGTAQEP"
     gene            complement(5477..>5606)
                     /locus_tag="VFD06_14715"
     CDS             complement(5477..>5606)
                     /locus_tag="VFD06_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HZN04834.1"
                     /translation="EPYLMQIGFLDRTPRGRRVTRRALEHLRLRPGGSTPGLFPEG"
BASE COUNT          761 a         1929 c         2013 g          903 t
ORIGIN      
        1 ggcgccggtc atgtacgcgg ccgagaacga ggacccgaag ttcccctcgt ccagctgcgc
       61 gtccaccggc ccgaggttgt cgttgtcgtt ggagcgcagc accgcgacac cggcggtcat
      121 cggcgtgccg atgaggtcga acgcgggcgc catgaccagc ggcgccatgc gcagcgactc
      181 ctgcgtggcc ggtccacgcg acgtgaagtt gttgatcgtc tgctcgcagt ccgtcgagcc
      241 gaagaacgtg aagcagtcat cggtcgagga cccgaccgag gtgatgttct tggcggtgga
      301 cggaggcgga atctggatgt cctgcgggat gttggagttc tcgtccagcg ccgtgccgtt
      361 gaacaggtcc ggaatcacgc ggagcgccag accgaggcgg ttcgtcccga cgagaccgcc
      421 gttgttgccg accggcacga acaccatgta gtcgcggttg ttgtacagga aggtgtcgac
      481 ctggaccgcc tcggccgggt acgtcccgtt gctggccagg aactggatcg tcgagaagtt
      541 cgacggggcg ccgaacggca tcaccgccag gtggccgtcc gcgccggcgc cggtgagggc
      601 gccgttcaga cggtcggtca gcgagccggg gctgacgttg ccgccgcgct ccaccagcga
      661 gttgatggtg cactgcgcca ccgtgccgac gtcctgcagg atgatgcggg cgccgcgcgc
      721 cacgccgtcc atgttctcgt tgcgcggctg ggtcggacgg gcgccgttgt tgccgcggct
      781 cgcgccgatg ccgaactggc tcgggtaggc ggcgatcgcc gacgcgacga tgtggccgtg
      841 cgttccggcc ccggagagct gcgcgtcgca ggacgtcccg gagtcgacca cgttctgaat
      901 ggcgtggatc ttccggtgct gggggccgat cggccgggcg gggatgacgg tcgtcacctg
      961 ggtggccgtc tgcgagaagt tcggggtgtc gatgctgatc ccgttgtccg tgacggtgac
     1021 gatctgcggc ctcacggggt cgacgccgtc gttgacgcgc tcgccgtcgg cgttactgtc
     1081 gatcccgccc ccgtccaccc cggccacgtc gaacggccgg atcaggttgg cgtcctcggc
     1141 cgaaccggcc tggatcgtcg ggacgttctc gtcgttaaac aggaccatct cgcggatttc
     1201 gtcgaccgtg acgatctcat cgatgcgcgc cagcttgtcg atcgagccgt aatggacctt
     1261 caggtggagc tctccgccgg tctcgttctc ggtcacctcg gagacgcccg gggtggcggc
     1321 caccttcttc gccatgtcgg tcgtggagac accggggatc agggccaccc tgacctgcag
     1381 cttggggtcg ctcgcccggc gcttctcgat gtacggacgg gcgccgaggt cggccgccag
     1441 cttgtcggcc ggctgcatgg cggtcgcctg atcgatggcg gacgactggc gcaggaggtg
     1501 catgttgcgc gcctccacgt tcaccagcag cgtgcgccgc ggcagcaccg agaggactgt
     1561 gccgatctcg gagaccgacg ccatgaccgc gtccagcccc tgctgatcga tggcgctctg
     1621 gctcaggcgg acatagttga gcccgggccg gacagagaag ccctggccct gcgtcagctc
     1681 cgactggccc caccggaggc ccggcttcac gctctggagc aacgcgtcat cgttcgggtt
     1741 ccagctccgg tccccgatcg gcgaaaacag gaggtcgtcc cccgccccgc cttgcaggcg
     1801 ccgaggcttg agcacccctc cgaacggtga gaactccgcc gggggcagag ttccctcggg
     1861 cagaacgatg ctcgacagtc gtcgagcctc gtcctgcgcg tgcttcaact cgtaggcgtc
     1921 ttcgccttcg atgtcgctgc gctgggtctt gtcggcccag agcacgatcg cgggcgtcgc
     1981 cagaagcagg gagaggccga aggcaacgat catgcggttg cgcttcgctc caggcgcgaa
     2041 gagtgcgttc atgcgggtcc tcctcggtgg gcaggaagtg tggtgcgttg gatgcaggcg
     2101 aatggctgat ctcgtctcga taccggatgc gcctcgtaag cgaggcgctg tccgcagttc
     2161 agagcatgtc gtgtcagcta cagagcaaca gacggcacaa aaacccggcg aatccggcga
     2221 tgtggaccgc cttgccgtgc gcccatatac cgtcaaggcc ggatcgtgtc aagaaaaagt
     2281 tgtcgcggaa aatcccgtga tcgtgccttt cggcgcgatt gacagcggtg tcggaatctc
     2341 cctaaaatgc cgcgcttgcg gccggttcca cggcccggcc accggcaagg gatcacgcgg
     2401 aggggaaccc cttgaaacat gtcttcggcg tcatgtcggc ggcggcggaa aaaatcccca
     2461 catcctttcc gctcgaaaaa aaatggttct ataccattgc ggtcggaggg ttggcgctgc
     2521 tgaccgcggc cggggcgaag gtggcagtcc cgcttccggg cacgccggtc ccggcgacgc
     2581 tgcagaccct gccggtgctc ctcgcggggc tgctgctcgg ctcccggatc ggcgccctga
     2641 gccaggtggt ctatctgacg ctcggcctcg ccgggctgcc ggtgttcgcg cttccggggg
     2701 ccggcccggc ctatctcctc ggcccgacag gtggttatct tctaggattt gtcgccgccg
     2761 catggatcac cggtgccctc gctcccccgt ccccgggccg cggcctgctg cgccggttcg
     2821 ccgccgtgat cgtggggatc gccgcgattc acgccgccgg cgtcctgtgg ctcgccctgt
     2881 tgttcgcgcc cgacacgttc gacgccttcc gagtcggctc cctgcccttc atcgccttcg
     2941 atctggcgaa ggggatcctc gcctgtgccg cagcggcggg ggccacgttc gcaaggagcg
     3001 ccctctggac gagccgctcc accgactgaa gacgatcctc gccggctgcg gagatgcgat
     3061 cgtcgctttc tccggcggcg tcgacagcac gctcctcctc aaggtggccc acgacgtgct
     3121 cggccctcgg gtgaccgcgg tcaccgcggt gtctgcctcc ctggcggcgg acgaaaggcg
     3181 ggagaccgag cgtctcgcag ccgggatcgg cgtcgcgcac cggttcgtcg agacgctgga
     3241 gatgggagac ccggactacg tcgcgaaccg ggggcggcgc tgttaccact gcaagcgcga
     3301 gctgttccgg gtgctgcggg cgatcgcctc gggggccggc catgatgtga cgctcctgta
     3361 cggggccatc gtcgatgatc tgggagacga gcgtcccggc atgcaggcgg ccgccgaggc
     3421 ccgggtgcgc gcccctctcc tcgaggccgg attcacgaag gagatgaccc gggatctctc
     3481 caggtcgctg gggcttccga actgggacaa gccggcgatg gcctgcctgg cgtcgcgcct
     3541 tccgatcggg accccggtga ccacggaggc cctccagcga atcgaccgcg cggaggcggc
     3601 ggtgcgccgg ctcggctacc ggcacgtccg cgtgcgggcg gcgggcgact cggcgcgcct
     3661 ggagctcgac ccggctgacc tgcgccgtgc cctcctggca ttcgagcggg aacggctcat
     3721 ttcggtcatc gtcgcagcgg ggttcgaaac cgtcgaagtg gacccgcgcg gctaccgtcc
     3781 cggcggccgc ggctaggcgc ctgtccgggt cctgcgtggg tcgcgcgcag gcggcttgcg
     3841 agcaccaggc gaggcgcgag gaggaaatcg atgcaggagc atcgatgacg acgagcagcg
     3901 aagcgtgggg ccgcaatccc catgcgccct gcgggttcgc cgctgcgaaa gcggcccacg
     3961 ccaggacccg gacaggctcc taagtgtcgc tcgcggacct gctgcggcgg cggcagtggc
     4021 cggtcccctt ccgccccgag ccggttcctc ccgtcgcact cctcgatcgc ctcatcgcgg
     4081 cggcgaaccc actgccgatg cgtcccggcg ccgggtgcgc cgcgtcgatc gtccgggcgg
     4141 gagcgccgcg acaggcggcg gccgccgccc tcgccgaggc gggtgtggac gcgaccgaac
     4201 tggcgcttct cgagccggcg gcggtgctct gcacggtcgc ctcccctgac agcgacggag
     4261 ccgcggcctg tcatctcggt gccggtcaga tcgccctcgc ggcggaggag gcggggctgg
     4321 gagcgctcgt cctgcccctc ccccggccga tccggatcga ggacggtccg gcgggccagg
     4381 tcttcgccgt ggtcgggatc ggaggctccc ccgtccgccc tccaggcccc gccccggcgg
     4441 gtgcgcgcgc cgagcagccc ggctcgccgg cggtgctcct gtcgctcctg gagatcgccg
     4501 cggccaccaa cgccgccgag gatctggacc gtctgctcga aagcatcgca caggagctgg
     4561 cccggctctt cccggtcgat cgggtcgatg cggcgttcgc ggaggagggt gtgattcagg
     4621 ccgccgtcat cgcgccgcgc gatccgtccg gccggcgcgc cgccgtcgat cgccttccgg
     4681 tggatgacac ccatcacctg ggggcggtcg tgtcgcaggg gcgggccctg tggcgcaacg
     4741 acctgacgat ggagttgcgc ttccgggaga cgctgccgcg aggaggcatg cgcagcgaca
     4801 tgaccatccc gctgcgctcg cggggtcagg ccactggcgc cctccggctg gcttcgcggc
     4861 gtccgcacgc ctacgaagcc gaggacttcg agatcctcga gcgcctggcc gacgtcatgg
     4921 cgatggcggt cgagaaccag cgtctcctgc aggtgacccg gcgcatggcg gaggtggacg
     4981 gcctcaccgg cgtctgcaac cggcgccact tccaggtcct cctggctcgg gaggcggagc
     5041 gggcccgatc ggcgggccat ccgctcggcc tcatgctcgc cgacgtcgat cgcttcaaga
     5101 gcgtcaacga ccgcttcggg caccccgccg gggacgcggt gctgcagcac gtggcgcggg
     5161 tcctcgggcg ccggctgcgc cgctcggacg tggtggcccg gtacggcggc gaggagttcg
     5221 cggtgctcct tcccggaacc gatcgggacg cggccgcctc cctggcggag gagctgcggc
     5281 gggaggtcga atcggccccc gccacgctcc cggcaccggc ggcccctctc gccgtgaccg
     5341 tgtcgctcgg ggtcgcgtcg atgccggcgg atgccttgga cgaaaacggg ctggtcaccg
     5401 cggccgatcg cgccctctat cgcgccaagg aagggggccg aaaccgggtc gagcgggccg
     5461 ggacggccca ggagccctag ccttccggga acagcccggg ggtcgagcct ccgggacgga
     5521 gcctcaagtg ctcgagggcc cgccgggtca cccggcgccc gcgcggcgtc cgatcgagga
     5581 aaccgatctg catgagatac ggttcg
//
LOCUS       DASRIQ010000595         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2056866, whole genome shotgun sequence.
ACCESSION   DASRIQ010000595 DASRIQ010000000
VERSION     DASRIQ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056866"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFD06_14720"
     CDS             <1..449
                     /locus_tag="VFD06_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZN04835.1"
                     /translation="MHFMNPVPIMALVEIVPGQATSNETLEETRALAVRLGKTPVVAQ
                     DFPGFISNRVLMPMINEAICALHEGVGTREDIDAVMTLGMRHPMGPLALADFIGLDTC
                     LAIMVVLFEGFGDPKYRPSPLLRRMVDAGWLGKKSGRGFYDYSGGK"
     gene            469..>3327
                     /locus_tag="VFD06_14725"
     CDS             469..>3327
                     /locus_tag="VFD06_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN04836.1"
                     /translation="MIPIAPDGMREFIARLRGAKKIGSDIVQVTTLPAEPAAFAPFAE
                     PLPAPLDGALRALGIERPYAHQAEAIDRVRRGEDVLTVTPTASGKSLVYLVPTFESAL
                     LRPGSRSLYLFPYKALAQDQLQGIEELSEAVALRGGRNRDSGHPIRAAIYDGDTDDAR
                     RRAIKADPPDILITNPDMLHLGILPYHESWRPFFETLDHVVLDELHVYRGIFGSHLHH
                     ILKRLMRVAAQYGRRPRVIASSATVAQPGRLGAALVGRPFSVVEKSGAPRAERHLMLL
                     NPTGSPYTAATSVFDAALDAGFRTIAFTKARKITELLHSWLLQGSPRWRGRVSAYRSG
                     YLPEERRDIERRLFSGDLRGVISTSALELGVDIGGLDVCLLVGYPGSIVSTWQRIGRV
                     GREDRPALTVLVALPDALDQYFMSHPAQLLERGFEEVVFDPGNPVVARAHLLCAGSEL
                     PIEAADREHYGDAGFELVRALEREGALALDAEGERWYGRRRRPQRDINLRSTGSTYAI
                     ADERSGRVIGTIDGVRAFHETHAGAVYLHHGQQFLVKELDVERRRVTVAPADLEYYTQ
                     VRGEKETAVLHVIEERGAGPLEARLGRIKVTEWITGYEKRRLHGRDRLGVFELTMPPQ
                     SFETVGLWLALPEALPDMVRERGLDFMGAIHAAEHAMISLFPLLAICDRGDIGGISYT
                     FHPEVGRPAIFIYDGHPGGVGLSAKGFRDLEDLIGKTAELLRDCPCDTGCPSCVQSPK
                     CGNGNQPLDKAGASLVLETLLGKVPLPAGRRAPRIQVIDPRDAEPVAAQPRHDGADGP
                     PSYSTTLFFDLETQKSAEEVGGWANIAAMKVALAVTYDDQTSRFRTYKEADINELLLD
                     LLTADQVIGYNIDRFDLEVLRGYTGRDLSRIRTRDMLADIYRKLGFRLRLADVAEATL
                     GTGKSSDGLQSLQWWKEGKIDLIEQYCRRDVELTRDLFL"
BASE COUNT          502 a         1188 c         1161 g          476 t
ORIGIN      
        1 ggatgcactt catgaacccg gtgccgatca tggcgctcgt ggagatcgtc ccggggcagg
       61 cgacgtcgaa cgagacgctc gaagagacgc gcgcactcgc cgtgcggctc ggcaagacgc
      121 cggtggtggc ccaggacttc cccggcttca tctccaaccg cgtcctcatg ccgatgatca
      181 acgaggcgat ctgcgccctc cacgaagggg tcggcacgcg cgaggacatc gacgccgtca
      241 tgacgctcgg catgcgccat ccgatggggc cgctggcgct cgccgatttc atcggcctcg
      301 acacctgcct cgcgatcatg gtggtgctgt tcgaggggtt cggcgatccg aagtaccggc
      361 cgtcgcctct cttgaggcgc atggtcgacg ccggctggct cgggaagaag agcggccgcg
      421 gcttctacga ctacagcggc gggaaatgaa cccggcgctc ggcgggtgat gatcccgatc
      481 gcgccggacg gcatgcggga gttcatcgcc cggctgcgcg gcgcgaagaa gatcggctcc
      541 gacatcgtcc aggtcacgac gctgccggcc gagccggccg cgttcgcccc gttcgccgag
      601 cctctgccgg cgccgctcga cggggcgctg cgcgcgctcg gcatcgagcg cccttacgcg
      661 caccaggcgg aagcgatcga ccgggtgcgt cgcggcgagg acgtgctgac ggtcacgccg
      721 accgccagcg gcaagagcct cgtctacctg gtccccacgt tcgagtcggc gctgctgcgc
      781 cccgggagcc ggtcgctgta cctgttcccc tacaaggcgc tggcgcagga ccagctgcag
      841 gggatcgagg agctgtcgga ggcggtggcg ctgcgcggcg ggcgcaaccg ggactcgggg
      901 cacccgatcc gggccgccat ctacgacggg gacacggacg acgcacgccg gcgggcgatc
      961 aaggcggacc cgcccgacat cctgatcacc aatccggaca tgctgcacct cggaatcctc
     1021 ccgtaccacg agtcgtggcg gccgttcttc gagacgctcg accacgtcgt cctcgacgag
     1081 ctgcacgtct atcgcggcat cttcggatcg cacctgcacc acatcctcaa gcgcctgatg
     1141 cgggtcgccg cccagtacgg acgacggccg cgggtcatcg cgtcctccgc caccgtggcg
     1201 cagccggggc gcctcggggc ggccctcgtc ggccgtccct tctcggtcgt cgagaagagc
     1261 ggcgcgccgc gggcggagcg ccacctgatg ctcctgaacc cgaccggcag cccgtacacg
     1321 gccgcgacct cggtcttcga cgccgcgctg gacgcggggt tccggacgat cgccttcacc
     1381 aaggcccgca agatcaccga gctgctccac tcctggctgc tgcaggggtc gccgcgctgg
     1441 cgggggcgcg tgagcgccta ccgctccggc tacctgccgg aggagcggcg cgacatcgag
     1501 cggcggctct tctcgggcga tctgcgcggc gtcatctcca cgagcgccct cgagctgggg
     1561 gtggacatcg gcggcctcga cgtgtgcctg ctggtcggct acccgggcag catcgtctcc
     1621 acctggcagc ggatcggccg ggtcgggagg gaggaccgcc ccgccctcac cgtcctcgtc
     1681 gcgcttccgg acgcgctcga ccagtacttc atgagccacc cggcacagct cctggagcgc
     1741 gggttcgagg aggtcgtctt cgatccgggg aacccggtcg tggcgcgcgc gcacctgctg
     1801 tgcgccggct ccgagctgcc gatcgaggcg gcggaccgcg agcactacgg cgacgcggga
     1861 ttcgagctcg tgcgggccct cgaacgggag ggggcgctgg cgctcgacgc ggagggggag
     1921 cgctggtacg ggcgacggcg gcggccgcag cgcgacatca acctgcgctc gaccggcagc
     1981 acctacgcca tcgccgacga acggagcggc cgggtcatcg gaaccatcga cggcgtgcgc
     2041 gccttccacg agacgcacgc cggggcggtg tacctgcatc acggccagca gttcctcgtg
     2101 aaggagctcg acgtcgagcg gcggcgggtg accgtggccc cggccgatct cgagtactac
     2161 acccaggtgc gcggggagaa ggagacggcg gtccttcatg tgatcgagga gcgcggcgcg
     2221 ggaccgctcg aagcccggct cggccgcatc aaggtgacgg agtggatcac cggctacgag
     2281 aagcggcgcc tgcacgggcg cgaccggctc ggcgtcttcg agctcacgat gccgccgcag
     2341 tccttcgaga cggtcggcct gtggctggcc cttccggagg cgctccccga catggtgcgg
     2401 gagcgcggcc tcgacttcat gggggcgatc cacgccgccg agcacgcgat gatttccctc
     2461 ttcccgctcc tggcgatctg cgaccggggg gacatcggcg gcatctccta caccttccat
     2521 ccggaggtcg gccggccggc gatcttcatc tacgacggcc atccgggcgg cgtcggcctg
     2581 tcggcgaagg gcttccgcga tctcgaggac ctgatcggga agaccgcgga gctcctgcgc
     2641 gactgcccct gcgacaccgg ctgcccctcc tgcgtgcagt cccccaagtg cggcaacggc
     2701 aaccaaccgc tcgacaaggc gggggcctcg ctggtgctgg agacgctcct cggaaaggtg
     2761 ccgctccccg ccggccggcg ggcgccgagg atccaggtca tcgacccgcg cgacgccgag
     2821 ccggtcgcgg cacagcctcg gcacgacggc gcagacggac cgccctccta ttcgacgacc
     2881 ctcttcttcg acctggaaac gcagaagagc gccgaggagg tcggcggctg ggcgaacatc
     2941 gcggcgatga aagtcgcgct cgccgtcacc tacgacgacc aaaccagccg gttccgcacc
     3001 tacaaggagg cggacatcaa cgagctgctg ctcgacctgc tgaccgccga ccaggtgatc
     3061 ggctacaaca tcgaccgctt cgacctcgag gtgctgcgcg gctacaccgg ccgcgacctg
     3121 tcgcgcatcc gcacgcgcga catgctcgcc gacatctatc gcaagctcgg cttccgcctg
     3181 cgcctggccg atgtcgccga ggcgaccctc ggcaccggca agagcagcga tggcctgcag
     3241 tcgctgcagt ggtggaagga ggggaagatc gatctcatcg agcagtactg ccgccgcgac
     3301 gtcgagctga cgcgcgattt gttcctg
//
LOCUS       DASRIQ010000596         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3640757, whole genome shotgun sequence.
ACCESSION   DASRIQ010000596 DASRIQ010000000
VERSION     DASRIQ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3640757"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFD06_14730"
     CDS             <1..478
                     /locus_tag="VFD06_14730"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HZN04837.1"
                     /translation="LARLLNLLVVPGTGLVPAVETGRTDIAGIFIPDVIKVDLSTPGA
                     RLAGGGSDHPTNPDDPGYSRLGIFGGDVLQSGIQDPFGNGGLIPGGWPNGRRFGDDVV
                     DIAVTALLSDLRTLPLFLQGPVGDGVDRNDVAFNKVFPYEPTPQNGRNHAHHGSMN"
     gene            583..1188
                     /locus_tag="VFD06_14735"
     CDS             583..1188
                     /locus_tag="VFD06_14735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN04838.1"
                     /translation="MQDALDTQDDRALMAGIAAREKTALETFYDRHAGQVMALCLRIL
                     RDRAEAEEVLGDVFWELWDRADRYRHDRGAPVAYVMTVARSRAIDRLRRRRPEVTASA
                     VETDAAGAPAPLADAPSADPGPFHAAAAAERRTRIRVALATLSEAERRAVELSFFDGL
                     SHGEIARALREPLGTVKSRIRQGLLRLRDALGDQYGTMGAA"
     gene            1185..2033
                     /locus_tag="VFD06_14740"
     CDS             1185..2033
                     /locus_tag="VFD06_14740"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZN04839.1"
                     /translation="MTPTPSDRMSCDERRDLLMLYAADALEPAEREALAAHLATGCPR
                     CAGGLAEAEATIAHLPLALEAVTPSESVRAALMLRVRSGARPVPFAPPAGAQPDDRSP
                     SRAGTWLRPTLAALAATMVTAVALSVPATQARRALLARLETQDREIVRLRGAVEAAAE
                     TVRVLRSPAVRVVSLSGTETQPQAAARIFWDRSRRTWHVYAAALRPPGPGRTYQLWFI
                     TPAKEKIPAATFDVDARGEAALDVVIPAGLDAIDVAAVTDEPTGGSPQPTGSIHLAGA
                     VAPTSS"
     gene            2080..>2810
                     /locus_tag="VFD06_14745"
     CDS             2080..>2810
                     /locus_tag="VFD06_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN04840.1"
                     /translation="MAPSRLAAAGLALAAAFLFLPPARAQEPPSKPTSSPPPTGSDVD
                     AIAADAVPGKGKSCILGKDLKPLSPRPRLALVLSGGGARGAAHIGVLKVLEELHVKPD
                     LIVGTSMGSIVGGLYAAGYSAEQVEGIMKTVDWKKIFVDKVDRTDRSYRRKQDDATFL
                     IPLRLRFKGWKPYLPSSLLGGQRLALLLRSLEIQATGADDFDDLTIPFRAVGANLADG
                     QSVVLEGGSLATALRASMAIAGLFPP"
BASE COUNT          361 a         1098 c          955 g          396 t
ORIGIN      
        1 gctggcgcgc ctgctgaacc ttctcgtcgt gcccggcacg ggcctggtgc ccgccgtcga
       61 gaccggccgc accgacatcg ccgggatctt cattcccgac gtgatcaagg tggacttgtc
      121 cacccccggc gcgcgtctcg ccggcggcgg atcggaccat ccgacgaacc ccgacgaccc
      181 cggctactcc cgcctcggca tcttcggcgg ggacgtcctg cagagcggca tccaggatcc
      241 cttcggcaac ggcggcctca tccccggcgg atggcccaac ggccggcgct tcggcgacga
      301 cgtggtggac atcgccgtca ccgcgctcct gagtgatctg aggacgctgc cgctcttcct
      361 ccaggggccg gtgggggatg gcgtggaccg caacgacgtc gccttcaaca aggttttccc
      421 gtacgagccc acgccgcaga acggtcgcaa tcatgctcat cacggttcga tgaactgatt
      481 tggccgtcag gtgaggcagg gggacgggtc ggaggcctcc cggcccgtcc cccttctgtt
      541 gtttcgcgcg caacccaggt actcttggac cggaaggtgg acgtgcagga cgctttggac
      601 acccaggacg atcgcgcact catggccggc attgccgcgc gggagaagac cgcgctcgag
      661 acgttctacg accgccacgc cggccaggtc atggccctct gcctgcgcat cctgcgcgac
      721 cgcgcggagg ccgaggaggt gctcggggac gtcttctggg aactgtggga tcgcgccgac
      781 cgataccggc acgaccgcgg cgcgccggtg gcatacgtga tgaccgtcgc acggagccgc
      841 gccatcgatc ggttgcggcg ccgccgcccg gaggtcacgg cgtcggctgt cgagaccgac
      901 gcggcgggcg ctccggcgcc gctcgcggac gccccctcgg cggaccccgg cccgttccac
      961 gccgccgccg cggccgagcg caggacgcgc atccgggtcg ccctcgccac cctgtccgag
     1021 gccgaacggc gcgccgtcga actgtcgttc ttcgacggtc tgagccacgg cgagatcgcc
     1081 cgcgccctgc gcgagccgct gggcaccgtg aagagccgca tccgccaggg cctgctgcgt
     1141 ctgcgcgacg ccctgggcga ccagtacggc acaatggggg ccgcatgacc ccgaccccga
     1201 gcgatcggat gtcctgcgac gagcgtcgcg acctgctgat gctctatgcc gccgacgcgc
     1261 tcgagccggc cgagcgcgag gcgctcgccg cgcacctcgc caccggctgc ccgcgctgcg
     1321 ccggcggcct cgccgaggcg gaggcgacaa tcgcccacct gccgctcgcc ctcgaggccg
     1381 tgacaccgtc cgagagcgtg cgcgccgcac tgatgctccg cgtccggtcc ggggcgcgcc
     1441 cggtgccgtt cgctcccccc gcgggcgcgc agcccgacga ccggtccccc tcccgggcgg
     1501 ggacctggct gcgcccgacg ctcgccgccc tcgccgcgac catggtgacg gccgtggccc
     1561 tgtccgtgcc tgccacgcag gcccggcgcg cgcttctggc acgtctggaa acgcaggacc
     1621 gcgagatcgt gcgcctgcgc ggcgccgtcg aggccgccgc cgagaccgtg cgcgtcctgc
     1681 gatcgcccgc ggtccgcgtc gtatccctct ccggcacgga gacgcagcct caggcggcgg
     1741 cccgcatctt ctgggatcgg tcgcgccgca cctggcacgt ctacgccgcc gcgctgcgcc
     1801 cgcccggccc cggccgcacg taccagctct ggttcatcac cccggccaag gagaagattc
     1861 ccgccgcgac cttcgacgtc gacgcgcgcg gcgaagcggc cctcgacgtc gtcatccccg
     1921 ccggcctcga cgccatcgac gtcgccgccg tcaccgacga gcccaccggt ggctcgccgc
     1981 agccgaccgg atcgatccac ctcgccggcg ccgtcgctcc gacgtcgtcc tgacactccg
     2041 gttgcgcccg ccgaacggtc acggctagga ttcgcctcca tggccccatc gcgcctcgcc
     2101 gcggccgggc tcgcgctcgc cgccgcgttc ctgttcctgc cgccggcgcg ggcgcaggag
     2161 ccgccttcga agcccacgtc ctcccccccg ccgaccggct ccgacgtgga cgcgatcgcg
     2221 gccgacgcgg tcccggggaa ggggaagtcc tgcatcctcg ggaaggacct gaagccgctc
     2281 tccccccgcc cgcgcctggc cctcgtcctg agcggcgggg gggcgcgcgg cgcggcccac
     2341 atcggggtcc tcaaggtgct cgaggagctg cacgtcaagc cggacctcat cgtcggcacg
     2401 agcatgggct cgatcgtcgg cgggctctac gccgccggct actcggccga gcaggtcgag
     2461 gggatcatga agacggtcga ctggaagaag atcttcgtgg acaaggtcga tcgcaccgat
     2521 cggtcgtacc ggcgcaagca ggatgacgcc accttcctga tcccgctgcg cctgcgcttc
     2581 aagggttgga agccgtacct gccttcgtcg ctcctcggcg gccagcgcct ggcgctgctc
     2641 ctccgttcgc tcgagatcca ggcgaccggc gccgacgatt tcgacgacct gaccattccg
     2701 ttccgcgccg tcggggcgaa cctcgccgac ggccagtcgg tggtgctgga aggggggagc
     2761 ctggcgaccg cgctgcgcgc cagcatggcg atcgcggggc tgttcccccc
//
LOCUS       DASRIQ010000597         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3641441, whole genome shotgun sequence.
ACCESSION   DASRIQ010000597 DASRIQ010000000
VERSION     DASRIQ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3641441"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFD06_14750"
     CDS             <1..322
                     /locus_tag="VFD06_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04841.1"
                     /translation="PRVLLATGTFPDPTVFNEDFAALSPALIDHLHEGGARLVGVDTP
                     SLDPCHSKDLPAHRACLRHDMAILEGLLLAHVPEGVYELIALPLRIAGADGSPVRAIL
                     RDLR"
     gene            complement(319..759)
                     /locus_tag="VFD06_14755"
     CDS             complement(319..759)
                     /locus_tag="VFD06_14755"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN04842.1"
                     /translation="MSRTKQKRDQDGEILSARAAEPVGPYPHARRAANLLFVSGTGPR
                     RRGQKETPGVTLDAQGKVASYDITAQCRAVFENLRFILEDAGSSWDRIVDVTAFLTNM
                     DDFPAYNAIYAEHFPENRPTRTTVQVSRLPGPIAVELKVIATID"
     gene            complement(779..2254)
                     /locus_tag="VFD06_14760"
     CDS             complement(779..2254)
                     /locus_tag="VFD06_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZN04843.1"
                     /translation="MREILNFIDGGLVPPLSGGWLDNVEPATGRVYGMVPDSDAQDVE
                     AAVRAAERAFPTWSRLPAEERARFLMALADRIEAAREDLARAESIDTGKPLALARSLD
                     IPRAASNLRFFATAAIHFHSESHSTGDRALNYTLRRPRGVAGVIAPWNLPLYLLTWKI
                     APALASGNTVVAKPTELAPMTAFLLAQLCRETGLPNGVLNIVHGRGAGAGAALVSHPR
                     VPAISFTGGTVTGSAIASVAAPLFKKVALEMGGKNPNIVFADADLDHAVATSLKAAFT
                     NQGEICLAGSRLFVEERIFETFVERFVAGARRLVVGDPLDERTDQGALISAAHRDKVL
                     GYLDVARQEGGRILCGGGPPDSIPSGRCRDGFFVAPTVIAGLPPTSRVNREEIFGPVV
                     TVTPFKSEDEVIGWANGTEYGLAASLFTTHLGRAHRVAERLASGTVWVNCWLLRDLRV
                     PFGGMKRSGVGREGGEEALRFFTEPKNVCVQMVPDSPTDKA"
     gene            complement(2342..>2604)
                     /locus_tag="VFD06_14765"
     CDS             complement(2342..>2604)
                     /locus_tag="VFD06_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZN04844.1"
                     /translation="WLDMEPQPLPGDSSMPRVQAPEFGASERMVVSPSHEEEGLFHMP
                     GGQSGHPLSKFYRAGHSAWATGQAAPFLPGPPEHVLILRPAR"
BASE COUNT          366 a          951 c          898 g          389 t
ORIGIN      
        1 gccgcgcgtg ctgctcgcca ccggaacgtt ccccgatccg accgtcttca acgaggactt
       61 cgccgcgctc tcgccggcgc tcatcgatca cctgcacgaa gggggcgcgc gcctcgtcgg
      121 cgtcgacacg ccgagcctcg atccgtgcca ctcgaaggac ctgccggcgc accgggcctg
      181 cctccggcac gacatggcga ttctcgaggg actgctcctg gcgcacgtgc cggagggggt
      241 gtacgagctg atcgccctgc cgctcaggat cgccggcgcc gacggcagcc cggtccgcgc
      301 catcctgcgg gatcttcgtt aatcgatcgt ggcgatgacc ttcagctcga cggcgatcgg
      361 ccccggcagc cggctcacct gcaccgtggt ccgcgtcggc cggttctcgg ggaagtgctc
      421 cgcgtagatc gcgttgtagg cggggaagtc gtccatgttg gtcaggaacg ccgtcacgtc
      481 cacgatccga tcccacgacg agccggcgtc ctcgaggatg aagcgcaggt tctcgaacac
      541 cgcccggcac tgcgcggtga tgtcgtacga ggcgaccttc ccctgcgcgt cgagcgtcac
      601 gccgggggtc tccttctgcc cgcggcgccg cggccccgtc cccgagacga acaggaggtt
      661 ggccgcccgg cgcgcgtgcg gatacggccc gaccggctcg gcggcgcgcg cgctcaggat
      721 ctctccatcc tggtcccgtt tctgcttcgt ccgactcatc cgctccccct cgtccgtgtc
      781 aagccttgtc cgttggtgaa tcggggacca tctgcacgca gacattcttc ggctcggtga
      841 agaagcgcag cgcttcctcc cccccttccc ggccgacgcc gctgcgcttc atgccgccga
      901 acggcacgcg caggtcgcgc agcagccagc agttcaccca caccgtgccg gaggcgagcc
      961 gctccgcgac ccggtgcgcc cgcccgaggt gcgtcgtgaa cagggacgcc gcgagcccgt
     1021 attcggtgcc gttcgcccac ccgatcactt cgtcctcgct cttgaacggc gtgaccgtca
     1081 ccaccggccc gaagatctcc tcgcggttca cccggctcgt gggcggtagc cccgcgatca
     1141 ccgtcggcgc cacgaagaag ccgtcccggc agcgcccgga ggggatggag tccggcgggc
     1201 cgccgccgca gaggatgcgg ccgccttcct gccgcgccac gtcgaggtag ccgagcacct
     1261 tgtcgcggtg cgcggccgag atcagcgccc cctggtcggt ccgctcatcg agcggatccc
     1321 cgaccacgag ccgccgcgcg ccggcgacga agcgttcgac gaaggtctcg aagattctct
     1381 cctcgacgaa caggcgcgat ccggcgaggc agatctcccc ctggttggtg aacgcggcct
     1441 tgaggctcgt cgccaccgcg tgatcgaggt cggcatcggc gaacacgatg ttcgggttct
     1501 tcccccccat ctcgagcgcc accttcttga agaggggcgc ggcgaccgag gcgatggcgg
     1561 agccggtgac cgtgccgccg gtgaacgaga tcgccggcac gcggggatgc gagacgagcg
     1621 ccgccccggc gcccgcgcca cgcccgtgga cgatgttgag cactccgttc ggcaggccgg
     1681 tctcgcggca cagctgcgcc aggagaaagg cggtcatcgg cgcgagctcg gtcggcttgg
     1741 cgacgacggt gttgccgctc gccagcgccg gggcgatctt ccaggtcagg aggtagagcg
     1801 gcaggttcca gggcgcaatg acgccggcga cgccgcgcgg ccgccgcagc gtgtagttga
     1861 gcgcccggtc gcccgtcgag tgcgactcgg agtggaaatg gatcgccgcg gtcgcgaaga
     1921 agcgcaggtt gctcgccgcc cgggggatgt cgagactgcg ggcgagcgcc agcggcttgc
     1981 cggtgtcgat gctctcggcc cgcgccagat cctcgcgcgc cgcctcgatc cggtcggcga
     2041 gggccatgag gaaccgcgcc cgctcctcag ccggcagccg cgaccaggtc gggaaggccc
     2101 gctccgccgc gcgcaccgcc gcctcgacgt cctgcgcgtc gctgtccggc accatcccgt
     2161 agacccgccc ggtcgccggc tcgacgttat cgagccaccc tcccgagaga ggaggaacga
     2221 gcccgccgtc gatgaagttg aggatttccc tcattggagg aatcgcctga ccacgcccag
     2281 accgtacgct gccgtcgccg caggcgtcaa gtgagccgcc ccgctctctc ggagcggagt
     2341 tttagcgcgc cgggcgcagg atgaggacgt gctcgggcgg cccggggagg aagggtgccg
     2401 cctgccccgt cgcccaggcg gaatgaccgg cgcgatagaa cttcgagagg ggatgcccac
     2461 tctgcccgcc gggcatgtgg aagagcccct cctcttcgtg gctgggcgac acgaccatgc
     2521 gttccgaggc gccgaactcg ggggcctgca cgcgcggcat gctgctgtcg cctggaagcg
     2581 gctgcggctc catgtcgagc cagg
//
LOCUS       DASRIQ010000598         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1548652, whole genome shotgun sequence.
ACCESSION   DASRIQ010000598 DASRIQ010000000
VERSION     DASRIQ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548652"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2613)
                     /locus_tag="VFD06_14770"
     CDS             complement(<1..2613)
                     /locus_tag="VFD06_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN04845.1"
                     /translation="MTRRDGRALLWLPVLLLVPGALLFGDRPRPSRHGEAEGPERAIA
                     SSLARVAERLRPAEPARPPPEFAALGHALAPRVLQGEAAHGFLLTNLGHVEWDPERDA
                     IASGMPAELRTPPEELSRVATGSLQRGLNYLRLSEAAIAARGHDTVASELQRHARILG
                     WLPDRTVIAFVEAHDREALARSTLVDRSRPLEAYHKISPRLGRLPRWSRAEAENPDLL
                     ARVALVPGLGGTGLLEELARLSGVSEVIPDAASEEGAQLRVRYDRIATLARRAEVLSI
                     EPVFDYLLANAENVPAVQAGSAEDTDFARPFDAAGVDGGGIDTNGDGRRVNDGSDLVP
                     PQLVTVTDNGISVDTPSFSQTATQVTTAGIPLGVLHRKIHAIQNVTDSGNSCDAPLSG
                     SGTHGHVVASIIAASSSVFGIYGNRSGIGGPSEPRNVNLDGVARGARIIVQDAGVPNA
                     CLLGTKVEPGGNTPGNLLDRLTLAICPANGSGTGACAGVVGGGGDAHLSILPFGVPNF
                     SDLDPEVVLEMGTYSLASSQIDRFLYNNRDYMVFAPSGNSGADPGQLRFGLGDRRIPD
                     LFDGEVIGVPEDYEATQVQPPATAKNLVAVGASGGDCFTAYGTFDCEGALAGFSSRGP
                     ATEPSLRMAPMLTAPGVDLAAGLFTNGITMFRSSDNDNNNPVEAQLDEANAGTSFSAA
                     FLTGAAAIVRDYFAQGFYPTGSRGPATDRMPGISGALVKASLAASADFSEDNIGTTGE
                     HDAGDHTLRRTRCIDLTPLNGAEVGVMCNSEQGYGRPVLTSVLPLANWSDDFVLHPSS
                     LRPREYPAAGLLVFDRWGTGEGVIDNGASVSRSHLFTVFGPKTVPTPSGGLAIAAAQL
                     RLALAWIDLPSPENS"
BASE COUNT          385 a          979 c         1016 g          393 t
ORIGIN      
        1 gctgttctcc ggcgagggga ggtcgatcca cgcgagcgcg aggcgcagct gcgcggccgc
       61 gatcgcgagc ccgcccgacg gcgtgggaac ggtcttcggc ccgaagaccg tgaagaggtg
      121 cgagcgggag acgctcgcgc cgttgtcgat caccccttct cccgtgcccc atcggtcgaa
      181 gacgagcagc cccgccgccg ggtattcgcg cggccggagc gacgaagggt gcaggacgaa
      241 gtcgtcgctc cagttcgcca gcggcaggac gctcgtcagg accgggcggc cgtacccctg
      301 ctcgctgttg cacatcacgc cgacctcggc cccgttcagc ggggtgagat cgatgcaccg
      361 ggtccggcgc agggtgtgat cccccgcgtc gtgctccccc gtcgtcccga tgttgtcctc
      421 gctgaagtcg gcggaggcgg cgagcgatgc cttcacgagc gcgccggaga tccccggcat
      481 ccggtccgtg gcgggaccgc ggctcccggt tggatagaac ccctgggcaa aataatcccg
      541 cacgatggcg gccgcgccgg tcaggaaggc ggcggagaag gacgtgccgg cattcgcctc
      601 gtcgagctgc gcttccacgg ggttgttgtt gtcgttgtcg ctgctgcgga acatggtgat
      661 gccgttggtg aacagtccgg cggcgaggtc gacgccgggg gccgtcagca tcggcgccat
      721 gcgcagcgac ggctccgtgg ccgggccgcg cgacgagaag cccgccagcg ccccttcgca
      781 gtcgaatgtc ccgtaggcgg tgaagcagtc gccgccggac gctccgacgg cgacgagatt
      841 cttggcggtc gccgggggct ggacctgcgt ggcctcgtag tcctcgggaa ccccgatcac
      901 ctcgccgtcg aagaggtcgg ggatgcgccg atcgccgaga ccgaagcgga gctgccccgg
      961 gtcggcgccg gagttccccg acggcgcgaa caccatgtaa tcgcggttgt tgtacaggaa
     1021 ccggtcgatc tggctcgacg cgagggaata ggtccccatc tcgagcacca cctccgggtc
     1081 gagatcgctg aagttcggca cgccgaacgg caggatcgag aggtgcgcgt cgccgccgcc
     1141 cccgacgacc cccgcgcagg ccccggtccc ggatccattc gcggggcaga tcgccagggt
     1201 gaggcgatcg aggaggttgc ccggggtgtt cccccccggc tccaccttcg tccccaggag
     1261 acacgcgttc gggaccccgg cgtcctggac gatgatgcgg gcgccgcgcg cgacgccgtc
     1321 gaggttgacg ttgcgcggct cggacggccc gccgatcccg gatcggtttc cgtagatccc
     1381 gaaaacgctc gacgaggcgg cgatgatcga ggcgacgacg tggccgtggg tgccgctccc
     1441 cgagagcggg gcgtcgcagg aattgccgga gtcggtcacg ttctggatcg cgtgaatctt
     1501 ccggtgcagg accccgagcg ggatccccgc ggtcgtgacc tgagtcgcgg tctgcgagaa
     1561 cgagggggta tcgacgctga tgccgttgtc ggtgaccgtg acgagctgag gcggcacgag
     1621 gtcgctgccg tcgttgacgc gccggccgtc cccgttggtg tcgatgccgc cgccgtccac
     1681 cccggcggcg tcgaacggcc gcgcgaagtc ggtgtcctcc gccgatcccg cctgcaccgc
     1741 cgggacgttc tcggcgttgg cgaggagata gtcgaacacc ggctcgatcg acagcacctc
     1801 cgcccgccgc gccagggtcg cgatgcggtc gtagcgcacc cgcagctgcg ccccctcctc
     1861 cgacgccgcg tcggggatca cctcgctgac tccggagagg cgggccagct cttcgaggag
     1921 accggttcca ccgagccccg ggacgagcgc cacgcgcgcc aggagatccg ggttctccgc
     1981 ctcggcgcgg ctccaccgcg gcagccggcc gagccgcggt gagatcttgt ggtaagcctc
     2041 gagcgggcgg ctgcgatcga cgagcgtcga ccgcgccagc gcttcccgat cgtgcgcctc
     2101 gacgaacgcg atgaccgtcc ggtccggcag ccatccgagg atgcgggcat ggcgctgcag
     2161 ctcggaggcc acggtgtcgt ggccccgcgc tgcgatcgcc gcttcggaga gccgcaggta
     2221 gttgaggccg cgctgaagcg atccggtcgc gacgcggctc aactcctccg gcggcgtgcg
     2281 cagctcggcc ggcatgccgg aggcgatcgc atcccgttcc ggatcccact cgacgtgccc
     2341 gaggttcgtc aggaggaagc catgggccgc ctctccctgc aggacgcgcg gcgcgagcgc
     2401 gtgcccgagc gccgcgaatt ccggcggcgg tctcgccggt tccgccggcc gcaggcgctc
     2461 cgccacccgc gcgaggctcg aagcaatggc tcgctccggc ccctcggcct ccccatgccg
     2521 ggaaggccgg gggcggtctc cgaacagcag cgcccccgga acgaggagca ggaccgggag
     2581 ccagaggagc gcccggccgt cacgacgagt cacgccgccc ccgcttacgg gcacgctgga
     2641 atgcccgagc ggctgacgat ccgcttcggg tggccggccg gcggacagtt gccgtagggg
     2701 gccggcgcca gcggcgcgcc ggtcagcgtc aggcagacct gtccttcggt cgcctgctgc
     2761 gtcgcggtcg ccg
//
LOCUS       DASRIQ010000599        12040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1297324, whole genome shotgun sequence.
ACCESSION   DASRIQ010000599 DASRIQ010000000
VERSION     DASRIQ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 12040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12040
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297324"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VFD06_14775"
     CDS             <1..1211
                     /locus_tag="VFD06_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN04846.1"
                     /translation="KTRIAAEQAERAGDLQKAAELRYGQLAALQKKLDAANEALRKRS
                     QGAPPMLKEEVDEEEIAAIVSRWTGIPVSRMLEGEVKKLLTMEERLRERVVGQDEAVS
                     LVSQAVRRARAGLADPNRPIGSFLFMGPTGVGKTELARALAEFLFDSEKAMIRLDMSE
                     YMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLFDEIEKAHPEVFNALLQIL
                     DDGRLTDGKGRVVDFKNTILVMTSNLAGAIIQSWPERDRKGLKQRVEQELKTHFKPEF
                     LNRVDEVVIFRALTREDLTHVVEIQAARLAGHLRDRGIALELNPALKAHLAEIGYDPE
                     YGARPLKRTLQRLVLDPLARGVLEGSFPPGSALRADWDAGRTEVTFTRVEEAAGAAPR
                     RAGGARGRKG"
     gene            1208..1654
                     /locus_tag="VFD06_14780"
     CDS             1208..1654
                     /locus_tag="VFD06_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZN04847.1"
                     /translation="MTTTRWDPFRDLMAIQERMNRLFQETLSRQQGNEDIQAGQWTPA
                     VDIHESPDRIVLRADLPGLEQSQIEVRVDDNTLTLRGDRRPPADVRQEEFHRAERPHG
                     AFMRSFSLPQGIDQAAIKASYKSGVLEVSLPKRQEARAASIRVDVK"
     gene            1677..2519
                     /locus_tag="VFD06_14785"
     CDS             1677..2519
                     /locus_tag="VFD06_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HZN04848.1"
                     /translation="MNAIKTAFFLGLLSALLLLIGGAIGGRGGVLVALGLAAAMNFFS
                     YWFSDKIVLKMHRAQPVTREQAPQLFAALERLTGRTGTPMPRVYMLGEEAPNAFATGR
                     DPQHAAVAVTQGLMGLLSAEELEGVLAHELAHVKNRDILISSVAATIASAVMVLASMA
                     RFAAIFGSGRGGDDRDGVNPFALLATALFAPIAAALIQMAVSRSREYQADESGAAMTH
                     NPYALASALQKLEDHGKRIPMHTASPQSSHLFIVKPFTGASLMSLFSTHPPIRERIRR
                     LTGR"
     gene            2532..3197
                     /locus_tag="VFD06_14790"
     CDS             2532..3197
                     /locus_tag="VFD06_14790"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZN04849.1"
                     /translation="MADGDKGAKGSGRGNGQDEAPGFSVVDRRPRYSPDEAGAEAGGE
                     TDAAGDPRPPAIVEELKARAEEAERRTREISAAYRRLDEERAALRERLGRDLERRLDA
                     ARADLMRRMLPVLDDLDRALAAAEAGAGGRSLKEGVALVRDRLMQALSAEGVEPVEIA
                     GRRFDPSIAEAVATEETDDPSRDQQVVEEMERGFRLRGALLRPARVKVARLKPPPGGA
                     IPE"
     gene            complement(3247..4164)
                     /gene="rocF"
                     /locus_tag="VFD06_14795"
     CDS             complement(3247..4164)
                     /gene="rocF"
                     /locus_tag="VFD06_14795"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HZN04850.1"
                     /translation="MEPHPRRIDLIGVPLDLGAGRRGVDMGPSAIRIAGLSAQLTELG
                     HTVVDRGDVPVPIPETIPVTGERKRYIAEIAAVCRRLAEMTFDAVSSERTPVCVGGDH
                     SLAAGSVAGAARALRGCGGELGLIWVDAHADMNTPETSPSGNVHGMPLAACLGQGPEE
                     LVRIGDGPSVRPENVALVGIRNIDACERDLVRKSGIKAYTMSDIDRRGVGPILDEILG
                     GLIRRTKGIHLSIDMDGLDPDLVPGVGTPVLGGLSYREAHLLAEMVAESGRLVSLDLV
                     ELNPILDVRNHSAEVGAGLILSALGQRIL"
     gene            4189..5040
                     /locus_tag="VFD06_14800"
     CDS             4189..5040
                     /locus_tag="VFD06_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017535195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZN04851.1"
                     /translation="MPTERIAIIGAGTMGHGIAQVAILSGFDVRLCDAAPGVLDRART
                     QIRAGLEKTIEKGRLDAAGRDAALARLTTGSDARPAVRDADLVIEAIPERLELKQALF
                     ATIEEAASPGCLLASNTSSLRIGAIAEGRRRPERILGMHFFNPVPVMSLLEIVVHEKT
                     SPEALEQAREVGVRLGKTCIVVQDSPGFASSRLGLALGLEAIRMVEQGVATPEDIDTA
                     MELGYRHPMGPLKLTDVVGLDVRLAVAESLHRELRSEAFRPPKLLVEMVREGRLGKKS
                     GRGFYEW"
     gene            complement(5072..6106)
                     /locus_tag="VFD06_14805"
     CDS             complement(5072..6106)
                     /locus_tag="VFD06_14805"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZN04852.1"
                     /translation="MSAPRAEIDPVHEPRSFAARARLYFAFTRPFTLLPPTFGVVSGA
                     LTAFGSAHNPDPERRFTTAVVLTIALGSLCAALLNAASNVLNQIHDLEIDRRNKPSRP
                     LPSGAISLEASRRFAFLLYVLAIVPTWLVVVYPYTDWQARLTAPLRAHECLFIYLAGM
                     ILTLVYSDPAWGRTKRFGLWANVTIAVPRGCLLKVAGWSMVGSVMHFEPWFIGSIFML
                     FLLGATTSKDFSDMEGDAAHGCRTLPIRWGVERAARFVAPFFVLPWLLLPLGAYLPDP
                     FEGGPILTGRPILLAILGLGLSIWGGFTARLILRDPRSLALRENHPSWKHMYLMMMCA
                     QIGFALAYLF"
     gene            complement(6103..7434)
                     /locus_tag="VFD06_14810"
     CDS             complement(6103..7434)
                     /locus_tag="VFD06_14810"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HZN04853.1"
                     /translation="MSAAHSPAARRIVHAGSGLLALLLKSLTVPQAALVAGGACLFNY
                     FVLPRIGASLFRSGERRRPLQSGIHIYPFAVLLLVLLFGWRMEVAAAAWVIFAAGDPA
                     ASFAGERFGRHRLPWNHEKSCAGTLAFLLAASLGGWAILVFMGRSGEEAVWLMVPTAL
                     FAAFVESLPWRLNDNLSVPLLSALFLCAFIEGDAIRLPEVAASLAPAFAIGAVVNALL
                     AALFLAVGAVDRSGAVGGFAVGTLTWGFGGWRAFAILVAFFVLGTLTTRLGRGRKERL
                     GVAQEKRGARSARHAIANCGVAVVLALLTATAATPGLFALAFVCAYATAAFDTVSSEV
                     GQAWGGKPVLITTLKRVPIGTDGAVSLLGTLAGLGAAALVGGLAVGLGMVPASLLPLV
                     LLAAFVGSSADSLLGATLERRGLLDNEAVNFSNTLIGALAGVVGGLLTIRL"
     gene            complement(7431..8312)
                     /gene="rfbD"
                     /locus_tag="VFD06_14815"
     CDS             complement(7431..8312)
                     /gene="rfbD"
                     /locus_tag="VFD06_14815"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HZN04854.1"
                     /translation="MSGSPAIRPLVIGAGGMLGRALVRRLESEFPDTIGATRAEIDIL
                     DRFRMEAEIERLEPTAVINCAAWTDVDGCTRDPARALEVNAEGAEHVARAAQGAGCRV
                     VQISTDFVFDGRLGRPYVEEDVTAPLQAYGLSKLEGERRVAVAAVDHLIVRTAWLYGE
                     HGDHFVGRILDRARRGEPLRVVEDQRGSPTLVDDLSDGIAALLRTDHIGIVHLVNGGV
                     ASRFELARAALAAEGLLDAVRLEATRTVVPPGGTARPAHSALDTSLFTRLTGVSPRPW
                     KDALEDYLGLAPGDAEA"
     gene            complement(8309..10936)
                     /gene="lptD"
                     /locus_tag="VFD06_14820"
     CDS             complement(8309..10936)
                     /gene="lptD"
                     /locus_tag="VFD06_14820"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HZN04855.1"
                     /translation="MPTPRAATAALLLAAALCPSLARAATPAPPPGEGTPQRQPFGTG
                     PGGCALEIAAGPGSVLDKDELVLKDYVDIKCGDLRLQADLVRYTPSTHEAHAEGNVVL
                     DQARVRITADSIDYNLETSTGQFFNARGYAEPSILFEAARIEQTSPEQMVLHDATFTA
                     CTQPTPYWSFKLGRGVLELGDYAYLHHLSFKLGKATIFYTPYLVWPIKTDRASGLLFP
                     EFGFSERSGTILSQAWYWAIGRSLDTTFYFDYMSESGYGFGNEFRYKPSETGRGQFTG
                     YYIRDQVAKEENQPGVPTDRWSIDYAHQQQLDPEWRFVTNMTFISDFDYFLDFERDIR
                     VSSNPQALSKAYLTRNWGFNSLNIWAERREQLVQDPIIPTAYGQPIIAFANRTFVRWT
                     EPEIEFRTRRRRIGDSPLFFTLESSVDYFDKGDRETVYGRGDLYPVISSQLSPVPWID
                     IDANAGLRDTFYTKSQRLDLGCDNVPDTGDFGEGNGLLDSERDNAPLGIFGAEDDLGC
                     DNLPATGDFGEGNNVRDLERTLIFDDSFDRRIYQAGLNLVGPKLSRIFDTPKSDFSPQ
                     YKNSIEPTLRYAYFSEVQDPLNIILFDDIDTLAGNTNRVTYGLSTRLYAKRPALGISS
                     LGFPMGPVGGAGSSQDALAHALERIRPPTPPAGEEPVVEGGTQPGAPKDKKLSTVEIA
                     SFEITQDYSFLGPLSASVALDEQRPVSPVMGTLRVNPSINTSVDLRTSFDVLFKEIRD
                     ASLSANLRSPGRGFLDFTWSISRDLEGKALQEQGVAFTNPFNRSQLGVHGEGVFFQRK
                     VLLGFQANYELGDLLPGEPRFRDQRYRAGYNTQCCGFQFEYLNRNFVGSDLTEFRFLV
                     NLRGVGNVVDLHQRVGAGG"
     gene            complement(10936..11742)
                     /gene="amrB"
                     /locus_tag="VFD06_14825"
     CDS             complement(10936..11742)
                     /gene="amrB"
                     /locus_tag="VFD06_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZN04856.1"
                     /translation="MIRRPAVAGMFYPAAEESLRSELQALTRGGSPADSAPLALLVPH
                     AGYVYSGRIAARTFLSGRLADRIVVLGPNHTGEGEAIAVQAEGAWRTPLGDAPIDAPL
                     AAAVLARVPRARIDALAHRREHSIEVQVPFLQHLHAGFRFVPICVGTHDLQALLDLGR
                     GLAEAIGEANERVLLVLSSDMTHYEPAEEAERRDRIALDRILALDPEGLLRVVRDESI
                     SMCGVAPAVAGLEAVRGLGAKSAALVAYGHSGERTGDDSSVVAYAGVAVH"
     gene            11894..>12040
                     /locus_tag="VFD06_14830"
     CDS             11894..>12040
                     /locus_tag="VFD06_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZN04857.1"
                     /translation="MRIALAQIAPPLGDPAGSLRLHLQTARRAVKAGADLVVFPELSL
                     TGYRL"
BASE COUNT         1902 a         4200 c         4322 g         1616 t
ORIGIN      
        1 tgaagacccg catcgccgcc gagcaggcgg agcgcgccgg cgacctccag aaggccgcgg
       61 agctgcgcta cgggcagctc gcggctcttc agaagaagct cgatgccgcc aacgaagcgc
      121 tgcgcaagcg ctcgcagggg gcgccgccga tgctcaagga ggaagtggac gaggaggaga
      181 tcgccgccat cgtctcccgc tggaccggca tccccgtgag ccgcatgctt gagggggagg
      241 tcaagaagct cctgaccatg gaggagcgcc tccgtgagcg agtggtcgga caggacgagg
      301 cggtgtcgct cgtcagccag gcggtgcgcc gcgcgcgcgc cggcctcgcc gatccgaacc
      361 ggccgatcgg ctccttcctg ttcatggggc cgaccggcgt cggcaagacc gagctggcgc
      421 gcgccctcgc cgagttcctg ttcgacagcg agaaggccat gattcgcctc gacatgtcgg
      481 agtacatgga gaagcactcc gtctcccgcc tcatcggcgc gccgcccggg tacgtcggct
      541 acgaggaggg ggggcagctc accgaagcgg tccgccgccg tccgtattcc gtcgtcctgt
      601 tcgacgagat cgagaaggcg cacccggaag tgttcaacgc cctgctgcag atcctcgatg
      661 acgggcgcct caccgatggc aaagggcggg tcgtggactt caagaacacc atcctcgtca
      721 tgacgtcgaa cctggccggg gcgatcatcc agtcctggcc ggagcgcgac cgcaagggac
      781 tgaagcagcg ggtcgagcag gagctgaaga cccacttcaa gccggagttc ctgaaccgcg
      841 tggacgaggt ggtgatcttc cgcgccctga cgcgggagga cctgacgcac gtggtcgaga
      901 tccaggcggc ccgcctggcc gggcacctca gggatcgcgg catcgcgctg gagctcaatc
      961 ccgccctcaa ggcgcacctg gcggagatcg gctacgatcc cgagtacggc gcgcgaccct
     1021 tgaagcggac gctgcagcgg ctggtgctcg acccgctggc gcgcggcgtg ctggaagggt
     1081 cgttcccgcc cgggtcggcg ctccgcgccg actgggacgc aggccgtacg gaagtgacgt
     1141 tcacgcgcgt cgaagaggcg gccggggccg ccccccggcg cgccgggggt gcgaggggga
     1201 gaaagggatg acgacgacgc ggtgggaccc gttccgcgac ctgatggcga tccaggagcg
     1261 gatgaaccgg ctgttccagg agacgctctc ccgccagcag gggaacgagg acatccaggc
     1321 gggccagtgg actccggccg tggacatcca cgagtcgccg gaccggatcg tcctgcgggc
     1381 cgacctgccg gggctggagc agtcgcagat cgaggtgcgg gtggacgaca acaccctgac
     1441 gctgcgcggc gaccgtcgtc cgccggcgga cgtgcggcag gaagagtttc accgcgccga
     1501 gcgcccgcac ggcgccttca tgcgatcgtt cagcctgccg caggggatcg accaggcggc
     1561 gatcaaggcg tcctacaaga gcggcgtgct cgaggtgagc ctgccgaagc ggcaggaagc
     1621 ccgggccgcc tcgatccgcg tcgacgtgaa gtgaagcgaa aggagactct cccccgatga
     1681 acgcgatcaa gaccgccttc ttcctcggcc tgctgtcggc cctgctcctc ctcatcggag
     1741 gggccatcgg cggccggggg ggcgtgctcg tggcactcgg cctcgccgct gccatgaact
     1801 tcttctccta ctggttctcc gacaagatcg tcctgaagat gcaccgggcg cagccggtga
     1861 cgcgcgagca ggcgccgcag ctcttcgccg cgctcgagcg gctgaccggc cggaccggca
     1921 cgccgatgcc gcgggtctac atgttggggg aggaggcgcc gaatgcgttc gccaccgggc
     1981 gcgacccgca gcatgccgcg gtcgccgtaa cccaggggct gatgggcctg ctgagcgcgg
     2041 aggagctgga aggggtgctg gcgcacgagc tggcgcacgt gaagaaccgc gacatcctga
     2101 tctcctcggt cgccgccacc atcgcgtcgg cggtgatggt gctggccagc atggctcgct
     2161 tcgccgccat cttcggaagc ggccggggcg gcgacgaccg ggatggggtg aacccgttcg
     2221 cgcttctggc cacggcgctg ttcgccccga tcgccgcggc cctcatccag atggcggtct
     2281 cccgctcgcg cgagtatcag gcggacgaga gcggcgccgc catgacgcac aacccgtacg
     2341 cgctcgcgag cgccctgcag aagctcgagg atcacggcaa gcggatcccg atgcacaccg
     2401 cctcgccgca gtcgagccac ctgttcatcg tcaaaccgtt cacgggcgcc agcctgatga
     2461 gcctcttcag cacccacccg ccgatccggg agcggatccg gcggctgacg ggacgctgac
     2521 ccgggacgct catggccgac ggcgacaagg gcgcgaaagg cagcggccgc gggaacggcc
     2581 aggacgaggc ccccggcttc tcggtcgtcg accgccgccc ccgctactcc cccgatgagg
     2641 ccggggcgga ggccggggga gagacggacg ccgcgggcga cccccgcccc ccggcgatcg
     2701 tcgaggagtt gaaagcccgc gccgaggagg cggagcgacg gacgcgcgag atctccgcgg
     2761 cctaccgccg tctcgacgag gagcgggccg ccctgcgcga acgactgggg cgcgatctcg
     2821 agcggcggct cgacgccgcg cgggccgatc tcatgcggcg catgctgccg gtgctcgacg
     2881 atctcgaccg cgccctcgcg gcggcggagg ccggcgccgg cggacggtcg ctcaaggaag
     2941 gggtcgcact cgtgcgcgac cggctgatgc aggccctctc cgccgaaggg gtggagccgg
     3001 tggagatcgc cggccggcgg ttcgatccgt cgatcgccga ggcggtggcg accgaggaga
     3061 ccgacgaccc gtcgcgggac cagcaggtgg tggaggagat ggagcgcggc ttccgcctgc
     3121 gcggcgcgct gctccgcccg gcccgcgtca aggtcgcgag gctgaagccg cctcccggcg
     3181 gcgcaatccc ggagtgaccg gcccggccga tccggccggc cgcgcgtgcg cccgggggtc
     3241 gcgcttctag aggatccgct gcccgagcgc cgacagaatg agtcccgccc ccacctcggc
     3301 ggagtggttg cgcacgtcga ggatcggatt caactccacg agatcgagcg acaccagcct
     3361 ccccgactcc gccaccatct cggccagcag gtgagcctcg cggtacgaca gcccgcccag
     3421 gaccggcgtg ccgacgccgg ggacgagatc cgggtcgagg ccgtccatgt cgatgctcag
     3481 gtggatcccc ttggtgcggc gtatcagccc cccgaggatc tcgtcgagga tcgggccgac
     3541 accgcggcgg tcgatgtcgc tcatggtgta cgccttgatg ccgcttttcc ggacgaggtc
     3601 ccgttcgcaa gcgtcgatgt tgcggatgcc gacgagggcg acgttctccg gccggacgct
     3661 cgggccatcg ccgattcgga ccagctcctc cggcccctgc ccgaggcacg cggccagggg
     3721 catgccgtgc acgtttcccg aaggggaggt ctccggagtg ttcatgtcgg cgtgcgcgtc
     3781 cacccagatc agccccagct caccgccgca gccgcgcagc gcgcgcgccg caccggcgac
     3841 cgatccggcg gcgagggagt gatcgccgcc gacgcaaacc ggcgtccgct ccgacgacac
     3901 cgcatcgaag gtcatctccg ccagccggcg gcagaccgcc gcgatctcgg cgatgtagcg
     3961 cttgcgctcc cccgtgaccg ggatcgtctc cgggatcggc accggcacgt cgccgcggtc
     4021 cacgacggtg tgcccgagct cggtcagctg cgccgacagg ccggcgatcc ggatcgccga
     4081 cggtcccatg tcgacgccgc gccggccggc gccgagatcg agcgggactc cgatgaggtc
     4141 gatgcgtcgc gggtgcggct ccacgggcgg attataattc gcatcccaat gccgaccgag
     4201 cgcatcgcca tcatcggggc cgggaccatg gggcacggca tcgcccaggt cgcgatcctg
     4261 tccggcttcg acgtccgcct gtgcgacgcc gccccgggcg tcctcgaccg ggcccggacg
     4321 cagatccgcg ccgggctcga gaagacgatc gagaagggac gcctcgacgc cgccggacgc
     4381 gacgccgcgc tcgcgcgcct caccacgggc tctgacgcgc gccccgccgt gcgcgatgcc
     4441 gacctcgtca tcgaggcgat cccggagcgg ctcgagctga agcaggcgct gttcgcgacg
     4501 atcgaggagg cagcctcccc cggctgcctc ctggcgagca acacgtcgtc gctgcgcatc
     4561 ggcgcgatcg cggagggacg gcgccgcccc gaacggatcc tcgggatgca cttcttcaac
     4621 ccggtgccgg tcatgtccct cctcgagatc gtcgtccacg agaagacctc cccggaggca
     4681 ctggagcagg cgcgcgaggt cggcgtccgg ctcgggaaga cctgcatcgt cgtgcaggac
     4741 tcccccggtt tcgcgtcgag ccggctgggc ctcgcgctcg gcctcgaggc gatccggatg
     4801 gtggagcagg gggtggcgac ccccgaggac atcgacaccg ccatggagct cggctaccgt
     4861 catccgatgg ggccgctgaa gctcaccgac gtcgtcgggc tcgacgtgcg gctggccgtc
     4921 gcggaatcgc tccaccgcga gctccggagc gaggcgttcc ggccgccgaa gctcctcgtc
     4981 gagatggtgc gggaagggcg gctcgggaag aagagcggcc gcggcttcta cgagtggtga
     5041 gccttccacc cggcggatcg gcgcatcccc tctagaacag gtacgccagc gcgaagccga
     5101 tctgggcgca catcatcatc aggtacatgt gcttccagga cgggtggttc tcgcggagcg
     5161 cgagcgatcg cgggtcgcgc aggatgagcc gcgcggtgaa gccgccccag atcgagagcc
     5221 ccaggccgag gatggccagc aggatcggcc gtccggtcag gatcggcccc ccctcgaacg
     5281 ggtcgggaag atacgccccg agaggcagca gcagccacgg cagcacgaag aacggcgcca
     5341 cgaatcgcgc ggcgcgctcc accccccagc ggatcggcag cgtccggcag ccgtgcgcgg
     5401 catccccctc catgtcggag aagtccttgc tggtcgtcgc cccgagcagg aacagcatga
     5461 agatcgagcc gatgaaccac ggctcgaagt gcatgacgct cccgaccatc gaccagccgg
     5521 ccaccttgag gaggcagccg cgcggcacgg cgatcgtcac gttggcccac agcccgaagc
     5581 gcttggtccg accccaggcc ggatccgaat agacgagcgt caggatcatc cccgcgaggt
     5641 agatgaagag gcactcgtgc gcccgaagcg gcgccgtcag gcgcgcctgc cagtcggtgt
     5701 acgggtagac gaccacgagc caggtcggga cgatcgccag cacgtacagg aggaacgcga
     5761 accgccgcga cgcctcgagc gagatggcgc cggacggcag cggccgcgac ggcttgttgc
     5821 ggcggtcgat ctccagatcg tggatctggt tcagaacgtt gctcgcggcg ttgaggagcg
     5881 ccgcgcacag cgatccgagc gcgatggtca ggacgacggc ggtggtgaag cggcgctccg
     5941 gatccgggtt gtgggcgctc ccgaacgccg tgagcgcccc ggacaccacg ccgaaggtcg
     6001 gcggcaggag cgtgaagggg cgcgtgaagg cgaagtacag acgcgcccgc gccgcgaacg
     6061 agcgcggctc gtggaccgga tcgatctccg cgcgcggcgc gctcacaggc ggatcgtcag
     6121 cagccccccg acgacgcccg cgagggcgcc gatcagggtg ttggagaagt tcaccgcctc
     6181 gttgtcgaga agcccccggc gctcgagcgt ggcgccgagg agcgagtcgg cgctcgagcc
     6241 gacgaaggcc gcgaggagca ccaggggcag gagcgacgcc ggcaccatcc ccaggccgac
     6301 cgcgagcccg ccgacgagcg cggccgcgcc gagcccggcc agcgtgccga ggagggagac
     6361 ggcgccgtcg gtcccgatcg gcacccgctt gagggtcgtg atcagcaccg gcttcccgcc
     6421 ccaggcctgc cccacctcgc tcgacaccgt gtcgaacgcg gccgtggcgt aggcgcagac
     6481 gaaggcgagc gcgaagaggc ccggggtggc ggcggtcgcc gtcaggagcg ccagcacgac
     6541 cgccacaccg cagttggcga tggcgtgccg cgccgagcgg gcgccgcgct tctcctgcgc
     6601 gacgccgagc cgctccttgc ggccccgccc gaggcgcgtc gtcagcgtcc cgagcacgaa
     6661 gaaggcgacg aggatggcga aggcgcgcca gccgccgaac ccccacgtca gcgtgcccac
     6721 cgcgaacccg cccaccgccc ccgagcgatc gaccgccccg acggcgagaa agagggcggc
     6781 gaggagggca ttgaccaccg ccccgatcgc gaaggccggc gccagcgacg ccgccacctc
     6841 cggcagtcgg atggcgtccc cctcgatgaa ggcgcacagg aacagcgccg acagcagcgg
     6901 caccgacagg ttgtcgttga ggcgccacgg cagggactcg acgaaggcgg cgaacagggc
     6961 ggtcggcacc atgagccaga ccgcctcctc ccccgaccgc cccatgaaga cgaggatcgc
     7021 ccacccaccg aggctggcgg cgaggaggaa tgcgagggtc ccggcgcacg acttctcgtg
     7081 gttccaggga aggcggtgcc ttccgaagcg ctcccccgcg aacgacgccg cggggtcccc
     7141 cgccgcgaag atcacccacg ccgcggccgc cacctccatg cgccatccga agagcagcac
     7201 caggagcagc accgcgaagg ggtagatgtg aatcccggac tggagcggcc gccgccgctc
     7261 gccgctccgg aagagcgatg cgccgatgcg cggcaggacg aagtagttga agagacacgc
     7321 gcccccggcg acgagggccg cctgcggcac ggtcaggctc ttgagcagga gcgccaggag
     7381 tcccgatccg gcatggacga tgcggcgcgc cgccgggctg tgcgccgcgc tcatgcctcc
     7441 gcgtccccgg gcgccaggcc gaggtagtcc tcgagcgcgt ccttccaggg acgcggcgac
     7501 accccggtga ggcgcgtgaa gagggacgtg tcgagcgccg agtgggccgg gcgcgccgtc
     7561 cctcccggag gcacgacggt gcgggtcgcc tccagccgca ccgcgtcgag gagcccctcg
     7621 gcggcgagcg ccgcacgggc cagctcgaag cgcgacgcga cgccgccgtt gacgaggtgg
     7681 acgatgccga tgtgatccgt ccggaggagc gccgcgatgc cgtccgagag atcgtccacg
     7741 agcgtcggcg agccgcgctg gtcctcgacc acccgcagcg gttcgccgcg cctggcgcga
     7801 tccaggatcc ggccgacgaa gtgatcgccg tgctcgccat agagccaggc ggtgcgcacg
     7861 atgaggtggt ccaccgccgc caccgccacg cgacgctccc cctccagctt ggagaggccg
     7921 tacgcctgga gcggcgccgt cacgtcctcc tcgacgtagg gccggccgag gcggccgtcg
     7981 aagacgaagt cggtgctgat ctgcaccacc cggcacccgg caccctgggc ggcgcgcgcc
     8041 acgtgctccg ccccctcggc gttcacctcg agggcgcggg cggggtcccg cgtgcagccg
     8101 tcgacgtcgg tccaggcggc gcagttgatc accgccgtcg gctcgagccg ctcgatctcc
     8161 gcctccatcc ggaagcggtc gaggatgtcg atctcggcgc gggtcgcgcc gatggtgtcc
     8221 gggaactccg actccagccg gcgcaccagc gcccgcccca gcatgccgcc ggcgccgatc
     8281 accagaggcc gaatcgcggg ggacccgctc atccgcccgc cccgacacgc tggtgcagat
     8341 ccacgacgtt gccgaccccc ctgaggttca cgaggaagcg gaactcggtc aggtcggagc
     8401 cgacgaaatt gcggttcagg tactcgaact ggaagccgca gcactgggtg ttgtagccgg
     8461 cgcggtagcg ctgatcgcgg aaacgcggct cccccggcag caggtccccc agctcgtagt
     8521 tcgcctggaa cccgagcagg accttgcgct ggaagaacac cccttcaccg tgcaccccga
     8581 gctggctgcg gttgaacggg ttggtgaacg cgaccccctg ttcctgcagc gccttcccct
     8641 cgagatcgcg gctgatgctc caggtgaaat cgaggaaccc gcgccccggc gagcgtaagt
     8701 tggcgctgag cgaggcgtcg cggatctctt tgaacaggac gtcgaacgag gtgcgcagat
     8761 cgacgctggt gttgatcgac gggttgaccc gcagcgttcc catcaccggt gagaccggac
     8821 gctgctcgtc gagcgccacc gaggcgctga ggggcccgag gaacgagtag tcctgagtga
     8881 tctcgaagga ggcgatctcg acggtgctga gcttcttgtc cttcggagcg ccgggctgcg
     8941 tccccccctc caccaccggc tcctcccccg cgggaggggt cggcggccgg atgcgctcga
     9001 gcgcgtgcgc gagggcatcc tgcgagctcc ccgcccctcc gaccggaccc atcgggaatc
     9061 cgagagagga gatgccgagg gccggccgct tggcgtacag tcgggtgctg aggccgtagg
     9121 tgacccggtt ggtgttgccg gccagcgtgt cgatgtcgtc gaacaggatg atgttgagcg
     9181 gatcctggac ctcggagaaa taggcgtagc gcagcgtcgg ctcgatgctg ttcttgtact
     9241 ggggggagaa gtcggatttc ggcgtgtcga agatgcggga cagcttcggc ccgacgagat
     9301 tgaggccggc ctggtagatg cggcggtcga agctgtcgtc gaagatcagg gtgcgctcga
     9361 ggtcccgcac gttgtttcct tccccgaaat cgccggtcgc ggggaggttg tcgcagccga
     9421 gatcgtcctc ggcgccgaag atcccgaggg gagcattgtc ccgctccgaa tcgagcaggc
     9481 cgttcccctc gccgaaatcc cccgtgtccg ggacgttgtc gcagccgagg tcgagccgct
     9541 ggctcttggt gtagaacgtg tcgcgcaacc cggcgttggc gtcgatgtcg atccacggga
     9601 ccggcgagag ctgcgaggag atcaccgggt aaaggtcgcc tcgcccgtag accgtctcgc
     9661 gatccccctt gtcgaagtag tcgaccgacg actcgagcgt gaagaagagg ggcgagtcgc
     9721 cgatgcgccg ccggcgggtg cggaactcga tctccggctc ggtccagcgc acgaaggtgc
     9781 ggttggcgaa ggcgatgatc ggctggccgt aggcggtcgg gatgatcggg tcctgcacca
     9841 gctgctcgcg ccgctccgcc caaatgttga gggagttgaa cccccagttg cgggtcaggt
     9901 acgccttgct gagcgcctgc gggttgctgc tcacccggat gtcgcgctcg aagtcgagga
     9961 aataatcgaa gtcgctgatg aacgtcatgt tggtgacgaa gcgccattcc gggtcgagct
    10021 gctgctggtg ggcgtagtcg atgctccagc gatcggtcgg aacccccggc tggttctcct
    10081 ccttcgccac ctggtcacgg atgtagtagc cggtgaactg gccgcggccg gtctccgagg
    10141 gcttgtagcg gaactcgttg ccgaagccgt agccggactc ggacatgtag tcgaagtaga
    10201 aggtcgtatc gaggctgcgg ccgatggccc agtaccaggc ttggctcagg atcgtgccgc
    10261 tccgttccga gaagccgaac tccgggaaga ggagtcccga ggcccggtcg gtcttgatcg
    10321 gccagacgag gtacggcgtg tagaagatgg tcgccttccc cagcttgaag gacaggtggt
    10381 gcaggtaggc gtagtccccc agctccagca cgccgcgccc gagcttgaac gaccagtagg
    10441 gcgtcggctg cgtgcaggcg gtgaaggtgg cgtcgtggag gaccatctgc tcgggagacg
    10501 tctgctcgat gcgcgccgcc tcgaacagga tggacggctc ggcgtagccg cgcgcgttga
    10561 agaactgccc ggtgctcgtt tccaggttgt agtcaatgct gtcggcggtg atccgcacgc
    10621 gcgcctggtc gaggacgacg ttcccctccg cgtgcgcctc gtgggtcgac ggagtgtagc
    10681 gcacgaggtc ggcctgcagg cgcaggtcgc cgcacttgat atcgacgtag tccttgagga
    10741 cgagctcgtc cttgtcgagg accgatccgg ggccggcggc gatctcgagg gcgcaccctc
    10801 ccgggccggt gccgaacggc tgccgctgcg gcgtcccctc ccctggaggc ggcgccggcg
    10861 tggcggcgcg ggcgagcgac ggacacagcg ccgcggcgag aaggagggcc gcggtcgcgg
    10921 cgcgcggcgt cggcatcagt ggacggcgac cccggcgtag gcgacgacgg agctgtcgtc
    10981 gccggtccgc tcgcccgaat gcccgtacgc cacgagcgcg gcgctcttcg ccccgagacc
    11041 ccgcaccgcc tcgaggccgg ccaccgccgg ggcgacgccg cacatgctga tcgactcgtc
    11101 gcgcaccacg cgcaggagac cctcggggtc gagggcgagg atgcgatcga gggcgatccg
    11161 gtcgcgccgc tccgcctcct ccgccggctc gtagtgcgtc atgtcgctgc tcaggacgag
    11221 gagcacccgc tcgttcgctt ctccgatcgc ctccgccagc ccgcggccga ggtccaggag
    11281 cgcctggaga tcgtgcgtcc cgacacagat cggcacgaac cggaatcccg catggaggtg
    11341 ctgcaggaac ggcacctgca cctcgatcga atgctcgcgg cggtgcgcca aagcgtcgat
    11401 gcgcgcccgc ggcacccgcg cgagaaccgc ggcggcgagc ggcgcgtcga tcggcgcgtc
    11461 cccgagcggc gtgcgccagg ccccttccgc ctgcacggcg atcgcctccc cttcccccgt
    11521 gtggttcggt cccagaacga cgatgcgatc ggccagcctt cccgacagga acgtccgcgc
    11581 cgcgatccgc ccggagtaga cgtagccggc gtgcggaacg aggagagcga ggggggcgga
    11641 gtccgccggg ctcccgccgc gggtgagcgc ctgaagctcg ctgcggagcg actcctccgc
    11701 ggccggatag aacatcccgg ccacagcggg ccttctgatc atcgccgtcc ccacccgtcg
    11761 gcgccccggg aaaacgactc gccgggggcc ctctccgggc cgcgcgatgc tagcaaacga
    11821 tcgctccaaa gtaaagaaac acaatcactt gcgttgatgc gccgcgggcc ggcgtgctac
    11881 aatccgcgac tccatgcgca tcgctttggc acaaatcgcc ccgcccctcg gcgatcccgc
    11941 gggaagcctc cggctgcacc tccagaccgc gcgccgcgcg gtgaaggcgg gggccgatct
    12001 ggtggtcttc ccggagctca gcctgaccgg ctaccgcctg
//
LOCUS       DASRIQ010000600         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2314098, whole genome shotgun sequence.
ACCESSION   DASRIQ010000600 DASRIQ010000000
VERSION     DASRIQ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2314098"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..911
                     /locus_tag="VFD06_14835"
     CDS             87..911
                     /locus_tag="VFD06_14835"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZN04858.1"
                     /translation="MRSRSRWLMLGLAAALTLLLAPALAGAQESSYVIGPEDVIEVQV
                     WDNKDLNQIVFVRPDGKTSLPLIGEIQAGGRTVQQLQDDLVKAYSRTVKVPSVTVIIK
                     EIKSRPVYFIGGFGRPGPMQLTRNDMTLLEATAMIGGVAPVGDAEKGFVLRGGKKIPV
                     NFEKMQKGDVSQNLKLEPFDSIVVPVAELVYVQGEVRAPGSVKYTADLTLAKAITQVG
                     GTTPMAAPGRVELLRSEGEKKVRMRVDLDKILRSPEDNPDVKLRPEDIIFVPQRLF"
     gene            925..2445
                     /locus_tag="VFD06_14840"
     CDS             925..2445
                     /locus_tag="VFD06_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04859.1"
                     /translation="MQGEPRGSRLPLPLLASLLVLAGPLLWAPLVIAQQTTPSGQTQA
                     PPPSQTQTPPQTGTTPSTQPTTGQQILGPDGKPFVPPTPPPPSQSRIPGAFDTSLIPA
                     SAPRFTFTPTLTLQEQWTDNFFLTETGRTENFRTTAAVGLDLLMNLPNTRGSLSTSLA
                     GSYDSAPDEEQANFFPSFTGSVQHTFSPRLSLTISDTFRRDDDPFLSDPNGVRRERDT
                     FISNTFSASVNWLIDIFQTQYYYRNNLFLSDDSDTISHILGGNVAVPLGAIHTLSGGY
                     EFTFRDSSGDSDQIGNRVYGSLSRQIGTFTSAGVSSSFSWISANTDSRIFNVSLFAAH
                     GIPGGFSVSGSVGYSLYDSETADQPTHLASFSLNASYRFARGIVSLGVFQDIRQTADE
                     GQDFGIVTTRSATFSASYSFTAFITGSIYARYSRNEPLEGGGSNLASSSTYLTAGANL
                     SWQITNWLSLTLAYLYIDRDSDNSTNIGSNDIPLSQSQFLQNSTENRATATLSARF"
     gene            2683..>3543
                     /locus_tag="VFD06_14845"
     CDS             2683..>3543
                     /locus_tag="VFD06_14845"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN04860.1"
                     /translation="MLRVFRHYIPHLPLILFAGDLAVLVVAFHVPKLTGTWIAEGALI
                     PRLVLVVFLVAFTLNLGGLYDIRTHIGRRELLARLLTCQAVAGLLVAAVAFAIPSLRL
                     GRGAFLQIGAAATLGLIAWRGAWIGPFSHRRIRIRVLVLGTGAIGKVIAGLVETGARP
                     FSIVGFLDDNPNAPETVPSPYALLGKVQDLPALVEETRPDLVVVAQMNRRGNFPAKAL
                     IDCRMRGIQVEDWPTFYEKETGKILVTDLRPSWLIFSDGFVKTPRTEIIKRLVDVALS
                     LAGLLMALPIM"
BASE COUNT          606 a         1258 c         1039 g          640 t
ORIGIN      
        1 tgaaggccta cgcggaggcc ggcatcgagg ctcccgccca acctaatcgg gccagcgagc
       61 ccgagcacct gctaggagtc tgatccatgc gcagccgtag ccgttggctc atgcttgggc
      121 tggccgccgc gctgaccctt ctcctcgcgc cggcgctggc cggcgcccag gaatcgtcct
      181 acgtgatcgg gcccgaggac gtcatcgagg tgcaggtgtg ggacaacaag gacctcaacc
      241 agattgtctt cgtgcggccc gacggcaaga cgtcgctgcc cctgatcggt gagatacagg
      301 cgggcgggcg cacggtgcag caactccagg acgacctcgt caaggcctac agcaggacgg
      361 tcaaggtccc ctcggtgacc gtcatcatca aggagatcaa gagccggccc gtctacttca
      421 tcggggggtt cggccggccg ggcccgatgc agctcacccg gaacgacatg acgctcctcg
      481 aggcgacggc catgatcggc ggcgtggccc cggtggggga tgccgagaag gggttcgtgc
      541 tgcgcggcgg caagaagatc cccgtgaact tcgagaagat gcagaagggc gacgtctccc
      601 agaacctcaa gctcgagccc ttcgactcga tcgtggtccc ggtggcggag ctggtctatg
      661 tccaggggga ggtccgggcc ccgggctccg tcaagtacac cgccgatctc acgctggcca
      721 aagcgatcac ccaggtcgga ggcacgaccc ccatggccgc gcccggccga gtcgaacttc
      781 tcagatcgga aggcgaaaaa aaggtgcgta tgcgggtcga ccttgataag atcctccggt
      841 cgccagagga taatccggac gtgaaactga gacccgaaga cataatcttt gtaccccaga
      901 ggctcttcta ggactgattg gccggtgcag ggggagccgc gaggctcgcg gctccccctg
      961 cccttgctgg cttccctcct cgtgctcgcg ggcccgctcc tctgggctcc tctcgtcatc
     1021 gcgcagcaga ccacgccctc gggacagacc caggcgccgc ccccgtctca gacgcagact
     1081 ccgccccaga cgggaacgac gccgtccacg caaccgacga ccgggcagca gatcctcggc
     1141 ccggacggca agcccttcgt gccgccgacc ccgccgccgc cctcgcagtc gcggattccc
     1201 ggggcctttg acacgtccct gatcccggcg agcgctccgc gtttcacgtt cacgccgacg
     1261 ctcaccctcc aggagcagtg gacggacaac ttcttcctga ccgagacggg gcggaccgag
     1321 aacttccgga ccaccgccgc cgtcggcctc gacttgctga tgaatctgcc gaacacgcgg
     1381 ggctccctgt ccaccagtct cgccggctcg tacgactcgg cgccggacga ggagcaggcg
     1441 aacttcttcc cgtccttcac cggctccgta cagcacacgt tcagcccccg cttgagcctg
     1501 accatcagcg ataccttccg ccgggatgac gatccattcc tgagtgaccc caacggtgtg
     1561 cgccgagagc gcgacacctt tatctccaac accttcagcg cctccgtcaa ctggctcatc
     1621 gacatcttcc agacccagta ctattatcgg aacaacctct tcctctccga cgactccgac
     1681 acgatcagcc acatcctcgg cgggaacgtg gcggtcccgc tgggcgccat ccatacgctg
     1741 agcggcgggt atgagttcac cttccgcgac tcctccggcg actcggacca gatcggcaac
     1801 cgcgtctacg gatcgctgtc ccgtcagatc ggtaccttca cgtcggcggg ggtgtcgtcg
     1861 tcgttcagct ggatcagcgc caacacggac tcgcggatct tcaacgtatc gctgttcgcc
     1921 gcgcacggca ttcccggcgg cttctcggtg tcggggagcg tcggatacag tctgtacgat
     1981 tccgagaccg cggaccaacc cacccatctc gcgtccttca gtctcaacgc cagctatcgc
     2041 ttcgcgcggg gtatcgtgag cttgggcgtc ttccaggaca tccgccagac ggctgacgag
     2101 gggcaggact tcggtatcgt gaccactcgc tccgcgacct tctccgccag ctactctttc
     2161 actgcattca tcacggggag catctacgcg cgctactcgc ggaacgagcc gctggagggg
     2221 ggtgggtcga acctagcctc ctcctcgacc tatctcaccg cgggggcgaa cctgagctgg
     2281 cagatcacca actggctatc cctgacgctc gcctacctct acatcgaccg cgactcggac
     2341 aactccacca acatcggctc gaatgacatc ccgctctccc agagccagtt cctccaaaac
     2401 tcgactgaga accgagcaac cgcgacgctc tccgcccgct tttagcagac ccctccacgc
     2461 aaagtctgca tgggggttcc cgcccgcaac tctctgattc ctggtcaaat ttccctcctc
     2521 ggtcgtttca ccacgcacat tcagcaggaa gggaactgga attccctata cgggtacgga
     2581 acttcactat tgtgatgacc atcgaagctt ttaaatcaaa tagttagaaa ctacgcggtc
     2641 cgttctactt ggcacttcga ttgctcccct ttcccgccgg tcatgctgag ggtattccgt
     2701 cattacatcc cgcatctccc gctcatcctc ttcgctggcg acctcgcggt gctggtggtg
     2761 gctttccatg tcccaaaact cacgggcacc tggatcgccg agggggcgct gatcccccgc
     2821 ctcgtgctcg tcgtgttcct cgtcgccttc actctgaatc tcggcggtct ctacgacatc
     2881 cgcacacaca taggtcgccg ggaactgctg gcgcgcctcc tcacctgcca ggccgtggcc
     2941 ggcttgctgg tcgcggcggt ggccttcgcg attcccagcc tgcggctggg ccgcggcgcc
     3001 ttcctgcaga tcggagctgc cgcgacgctg ggcctcatcg cctggcgcgg cgcctggatc
     3061 ggcccgttct cccaccgccg catccggatc cgagtgctcg tcctcgggac gggcgcgatc
     3121 gggaaggtga tcgcgggcct cgtggagacg ggagcgcggc cgttctcgat cgtcggcttc
     3181 ctcgacgaca atcccaatgc gccggagacg gtgccctcgc cctacgccct tctcggcaag
     3241 gtccaggacc tgccggcgct ggtcgaggag acgcggccgg acctggtcgt ggtggcccag
     3301 atgaatcggc gcgggaactt ccccgccaag gccctcatcg attgccggat gcgcggcatc
     3361 caggtcgagg actggcccac gttctacgag aaggagacgg gcaagatcct cgtcacggac
     3421 ctccggccga gctggctgat cttctccgac ggcttcgtca agacgccccg gaccgagatc
     3481 atcaagcgac tcgtcgacgt cgcgctgtcc ctcgccggcc tgctcatggc cctgccgatc
     3541 atg
//
LOCUS       DASRIQ010000601         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2576333, whole genome shotgun sequence.
ACCESSION   DASRIQ010000601 DASRIQ010000000
VERSION     DASRIQ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2576333"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3222
                     /locus_tag="VFD06_14850"
     CDS             <1..3222
                     /locus_tag="VFD06_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04861.1"
                     /translation="GDCAGVTVVSRCRGNAAACPTPETTHPVCANSFGLGDLCTSYVA
                     NSGGCSSENDDGDADGVQDLADSCPASPNPGVVAGGPQPDRDRDGLGDACDPAGAHDD
                     AADGVPDDVVTFQGALACSTLPLVRLAPAGSPVYLDLDGDHDTFPDTGERGRVAITLV
                     NIGDGLQDAVFTLTSTDPDVACITESQIRVASFPAGATLTVGSLDPGQPGFTFVASNA
                     LQALPPPALPARVDLSLQVIAAGHLGATTPIAISLLADLNAPGAPQQFVLGADGIAGT
                     ADDGVMAEKFDQDRDGDGKFTVRDTFLTTTAPGVHRGTCSNAPLTVCVSAADCPAGPP
                     DPVCQSGAYLRGSATGSEPNRLAAVTCGGYNTAPADPQCALDPDFPMDWHLHCPSGTA
                     SCPNLESGTCVGGCSFNTPTGGTKALSLPNSLHMGAHFVPSDPLSGDTTHLRTVQGFM
                     TSPINLAVTPRPGDLDFSFFHIARLMDNNGVSASGHTCVDCGDVQIQIDQNLDPGVDA
                     WGFWDKLAPYQNVYDHRPMAWSEFGQTYCPFTPADTGTAPPSPRGVHETLCYPQGAWS
                     SCGSTTATVATSVGDCAGPGNLDPSGTGVWVQTRFHLEPYLGQRIRIRWIAETWNFGP
                     DFDSYYEFGGTWGPTLRDDGWWLDDIQLTGAVQAQVTPTADNTPRTGTCPGDPCDSTL
                     GDSGTSAAIEVTDPAGAVIDGVTRIPTSGAPIRLSAAGSTLPGGCANGHAEYQFLRDG
                     LVAQAWSANPFFFDSPERTTRYTALVRCTSDPGCASVVGASIDLPVRTGEGGDVVFGA
                     RGTLFDPGVGVAYYRGVCSGKVGLPCNQASDCSGGACDVTASTADDTTVLRLWAASDD
                     GLDVVRGTVPSGPAPKGTVAGSFWNLAGLSGPCFLSNLAPAAAATGFNYGAGPLTQAQ
                     DANPPLGGVIYYQASANSTGGQNLDAYGCPSPSICSNPGWCELGTNAGLPCTTNANCP
                     GGTCVASASFCSTDAGSAGQGGCGHHSTCAGGSSPGKLCQSAVDCPGGGTCPAPTTAQ
                     QAAEGQVCLTLTGAPLAPAPYGNCPPAGHPKRVVSRAGAPPCP"
BASE COUNT          459 a         1227 c         1103 g          453 t
ORIGIN      
        1 ggcgactgcg ccggcgtgac ggtggtgtcc cgctgccgcg gcaacgccgc cgcctgcccg
       61 accccggaga cgacccatcc ggtctgcgcg aactccttcg gtctcgggga cctctgcacg
      121 tcttacgtcg ccaactccgg cggctgctcc tccgagaacg acgacggtga cgcggacggc
      181 gtgcaggacc tcgccgactc ctgtcccgcg agcccgaacc cgggtgtggt ggcgggcggg
      241 ccgcagcccg atcgcgatcg ggacggtctg ggtgacgcgt gcgatccggc cggggcgcac
      301 gacgacgccg cggatggggt gccggacgac gtcgtgacct tccagggggc gctcgcctgc
      361 tcgaccctgc cgctggtccg cctcgcgccg gccggcagcc ccgtctacct ggacctcgac
      421 ggcgatcacg acacgttccc cgacaccggc gagcgcggcc gcgtggccat cacgctcgtc
      481 aacatcgggg acgggcttca ggatgcggtg ttcaccctga cctccaccga tcccgacgtc
      541 gcctgcatca ccgagtcgca gatccgggtc gccagcttcc cggcgggcgc gacgctgacg
      601 gtcggcagcc tcgatcccgg ccagcccgga ttcaccttcg tggccagcaa cgccctgcag
      661 gccctgccgc cgccggcgct gccggcgcgc gtggacctga gtcttcaggt gattgccgcc
      721 gggcacctcg gagccacgac gccgatcgcc atctcgctgc tcgcggatct caacgcaccg
      781 ggagcgccgc agcagttcgt cctcggggcc gacggcatcg ccggcacggc ggacgacggc
      841 gtgatggcgg agaagttcga tcaggatcgc gacggcgacg gcaagttcac cgtgcgcgac
      901 accttcctca cgacgaccgc gcccggcgtg catcgcggca cgtgcagcaa cgcgccgctg
      961 accgtctgcg tcagcgcggc cgactgcccc gccggcccgc ccgacccggt ctgccagtcg
     1021 ggggcctatc tgcgcggcag cgccaccggt tcggagccca accggctcgc ggccgtgacg
     1081 tgcgggggat acaacaccgc tcccgccgat ccgcagtgcg ccctggatcc cgactttccg
     1141 atggactggc atcttcactg cccgtccggc acggcctcct gccccaacct cgagtccggg
     1201 acctgcgtcg gcggctgttc cttcaacacg ccgaccgggg ggacgaaggc cctgagcctg
     1261 cccaactcgc tgcacatggg ggcgcacttc gttccgtccg atccgctctc cggagacacg
     1321 acccatctgc gaacggtgca ggggttcatg acttcgccga tcaacctggc ggtcaccccg
     1381 cgccccgggg atctcgattt ctccttcttc cacatcgcca ggctgatgga caacaacggg
     1441 gtctccgcca gcgggcatac gtgcgtcgac tgcggcgacg tgcagatcca gatcgaccag
     1501 aacctcgacc ccggcgtgga tgcatgggga ttctgggaca agctcgcccc gtaccagaac
     1561 gtctacgatc acagaccgat ggcctggtcc gaattcgggc agacgtactg cccgttcact
     1621 ccggccgaca ccgggacggc gccgccgagt ccgcgcggcg tccacgagac gctgtgctac
     1681 ccgcagggag cgtggtccag ctgtggctcc acgaccgcga cggtcgcgac ctccgtcggc
     1741 gactgcgccg gcccggggaa cctcgatcct tccggcaccg gcgtctgggt gcagacgcgc
     1801 ttccatctcg agccttacct cggccagcgg atccgcatcc gctggatcgc cgagacctgg
     1861 aacttcggcc ccgacttcga ttcgtactac gaatttgggg gcacctgggg ccccaccctg
     1921 cgcgacgacg gctggtggct ggacgacatc cagctgaccg gcgcggtcca ggcccaggtg
     1981 accccgaccg ccgacaatac gccgcggacc ggaacctgcc ccggggatcc ctgcgattcg
     2041 accctcggcg actccgggac gagcgccgcc atcgaggtga ccgatccggc gggggcggtg
     2101 atcgacgggg tgacccgcat cccgacatcc ggcgcgccga tccgcctcag cgccgccggc
     2161 tcgacgctcc cgggcggctg cgcgaacggg cacgcggagt atcagttcct gcgcgacggg
     2221 ctcgtggcac aggcgtggag cgccaacccg ttcttcttcg acagcccgga gcggacgacg
     2281 cgctacaccg cgctcgtccg ctgcaccagc gatccggggt gcgccagcgt cgtcggcgcc
     2341 tcgatcgatc tgccggtgcg gacgggcgaa ggcggggacg tcgtcttcgg agcgcgcggc
     2401 acgctcttcg atccgggggt cggggtcgcg tactaccgcg gcgtgtgcag cgggaaagtc
     2461 gggttgccgt gcaaccaggc ctccgactgc agcgggggag cctgcgacgt cacggcgtcc
     2521 acggcggacg acaccaccgt gctgcgcctc tgggccgcct ccgacgacgg cctcgacgtg
     2581 gtccgcggca ccgtgccgtc cggaccggcc ccgaagggga cggtcgccgg atcgttctgg
     2641 aacctcgcgg gactctccgg cccctgcttc ctgtcgaatc tcgccccggc cgcggccgcg
     2701 accgggttca actacggtgc cggcccgctg acgcaggcgc aggacgccaa tccacccctg
     2761 ggaggcgtca tctattacca ggcgtccgcc aacagcaccg gcggccagaa cctggacgcc
     2821 tacggctgcc ccagcccgtc gatctgcagc aacccgggct ggtgcgagct ggggacgaac
     2881 gccggccttc cgtgcacgac gaacgcgaac tgccccggtg gcacgtgcgt ggcatcggcc
     2941 agcttctgct ccacggacgc cggaagcgcg gggcagggag gctgcggcca tcactcgacc
     3001 tgcgccggcg ggagcagccc cgggaagctg tgtcagtcgg cggtcgattg tcccggcggg
     3061 ggaacctgtc cggcgcccac gacggcgcag caggcggccg aaggtcaggt ctgcctgacc
     3121 ctcaccgggg cgccgctggc gccggccccc tacggcaact gtccgccggc cgggcacccg
     3181 aagagggtcg tgagccgcgc cggcgcgccg ccctgcccgt aggaagccaa tcccccggcg
     3241 cc
//
LOCUS       DASRIQ010000602         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1550279, whole genome shotgun sequence.
ACCESSION   DASRIQ010000602 DASRIQ010000000
VERSION     DASRIQ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550279"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFD06_14855"
     CDS             <1..371
                     /locus_tag="VFD06_14855"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN04862.1"
                     /translation="PWEIDLVLEMSGAPDALDAGLRAVRRGGKMVLFGLPKENAVTLA
                     RYSEDVIFGGVTLKGIIGRKLFDTWERTRALTGRREVREKIQSVITHTYPLSRYEEAF
                     ARMIGRDSGKVVMTVGEEGR"
     gene            368..1555
                     /locus_tag="VFD06_14860"
     CDS             368..1555
                     /locus_tag="VFD06_14860"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZN04863.1"
                     /translation="MNQRLERFLEAEIAGLREKGIFRLPRVLSTPALPRVRLDGRDVI
                     QLSSNNYLGLTTHPRVVAAARRALEEFGAGPGAVRTIAGTMTLHRSLEAALARFKGTE
                     EALVFQSGYTANVGVVSTLMQEGDLILSDELNHASIIDGCRLSKAGRGVYRHLDYDHL
                     RELLKTARAGNVNRILVVADGVFSMDGDISDLKAIAALCEEFDAIPMVDDAHATGVMG
                     TNGRGTVDHFGLKDRWDIQIGTMSKAFGVMGGYVCAARRLVEFYVHKARPFLFSSSHP
                     PAVVAAAQAAVEVMETEPDLHRRLWENTRHFKAGLKQLGFNTGTSVTPITPVIIGSGA
                     KAARFSDALFEAGVFAQGIYFPMVAEEKSRVRTIVMATHTREDLDEALAAFEKVGRSL
                     GVV"
     gene            complement(1620..2042)
                     /locus_tag="VFD06_14865"
     CDS             complement(1620..2042)
                     /locus_tag="VFD06_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZN04864.1"
                     /translation="MAEAIPRKLFYKLQEVCELTDTQPYVLRFWESEFTQLAPTKSRN
                     GQRLYRKRDIDLVLEIKRLLNDEGQTIAAAREKLGMGEGPAPFLEELFEPPPAAEPRA
                     PAATAAPVKDTASREVADGLREILGLLDATDRRLSGGD"
     gene            complement(2128..2505)
                     /locus_tag="VFD06_14870"
     CDS             complement(2128..2505)
                     /locus_tag="VFD06_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN04865.1"
                     /translation="MTPRRIPKPWGEEILFAHTDRYAGKVLTVKRGESLSLQYHERKD
                     EALYLFSGQVELTLGAPGDAAGSAGSERRLLQAGDAIHLPPGTRHRMEALEDAILFEV
                     STPELDDVVRLEDRYGRAGTREP"
     gene            complement(2502..>3203)
                     /gene="rapZ"
                     /locus_tag="VFD06_14875"
     CDS             complement(2502..>3203)
                     /gene="rapZ"
                     /locus_tag="VFD06_14875"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZN04866.1"
                     /translation="AVLVVDVREGDLLKDFPEIYARVKSLPGGRTRLIFFDADDDTLI
                     RRFSETRRPHPLAPHASLEDGIAREREMLRPLRELADLTLDSSNFNVHQLRRYIQEHL
                     APLEGGSPMAVILLSFGFKHGLPIEADLVFDTRFLPNPFFVEGLRGRTGLDAEVVRYL
                     DGSSEYGDLRRRLVDLLTFLIPQYIREGKSYLTVAIGCTGGRHRSVALAEGVASDLGT
                     RGLLVRTSHRDLERE"
BASE COUNT          470 a         1117 c         1144 g          472 t
ORIGIN      
        1 ccccatggga gatcgacctc gtgctggaga tgagcggggc gccggacgcc ctcgacgccg
       61 ggctgcgcgc ggtgcggcgc ggcggcaaga tggtgctctt cggtctcccg aaggagaacg
      121 ccgtcacgct ggcgcgctac agcgaggacg tgatcttcgg cggcgtcacc ctcaagggga
      181 tcatcggccg caagcttttc gacacctggg agcgcacccg cgccctgacc ggccggcgcg
      241 aggtgcggga gaagatccag tcggtcatca cccacaccta tcccctgtcc cgctacgagg
      301 aggcgttcgc gcggatgatc ggccgggaca gcggcaaggt ggtcatgacc gtcggggagg
      361 agggacgatg aaccagcgtc tggaacgctt cctcgaggcg gagatcgccg gcctcaggga
      421 gaaggggatc ttccgcctgc cgcgcgtcct gtcgacgccg gcgctccccc gcgtgcggct
      481 cgacggccgg gacgtcatcc agctctcctc caacaactac ctcgggctga cgacgcatcc
      541 gcgcgtcgtg gcggcggcgc gccgggccct cgaggagttc ggcgccgggc cgggggcggt
      601 ccgcaccatc gcaggcacca tgacgctgca ccgctccctc gaggcggccc tcgcccgctt
      661 caaggggacc gaggaggcgc tcgtcttcca gtccggctac accgcgaacg tcggcgtcgt
      721 ctccaccctc atgcaggagg gagacctcat cctgtccgac gagctgaacc acgccagcat
      781 catcgacggc tgccgcctga gcaaggcggg ccgcggtgtc taccggcatc tcgactacga
      841 ccacctccgg gagctcctga aaacggcgcg cgccgggaac gtgaaccgga tcctcgtcgt
      901 cgcggacggg gtcttctcca tggacggaga catctccgat ctgaaagcga tcgcggcgct
      961 ctgcgaggag ttcgacgcca tcccgatggt ggacgacgcg cacgccaccg gcgtgatggg
     1021 gacgaacggg cgcggcacgg tcgaccactt cggcctcaag gatcgctggg acatccagat
     1081 cggcacgatg tcgaaggcgt tcggggtgat gggggggtac gtctgcgcgg cgcgccggct
     1141 cgtcgagttc tacgttcaca aggcgcgccc gttcctcttc tcctcgtcgc acccgccggc
     1201 ggtggtggcg gccgcgcagg cggccgtcga ggtgatggag accgagccgg acctgcaccg
     1261 gcggctctgg gagaacaccc gccacttcaa ggcggggctg aagcagctcg ggttcaacac
     1321 cggcacctcg gtcacgccga tcaccccggt gatcatcggc tcgggggcga aggcggcccg
     1381 cttcagcgac gcgctgttcg aggccggcgt gttcgcgcag ggcatctact tcccgatggt
     1441 ggcggaggag aagtcgcgcg tccgcaccat cgtcatggcg acccacaccc gggaggacct
     1501 ggacgaggcg ctcgccgcgt tcgagaaggt cgggcgctcg ctcggcgttg tctaagttga
     1561 gcgcgccggc ggctgctggc gcttggctcg agcgcgccgg cctgctgccg gcgccggcgc
     1621 tagtcgccgc cggagaggcg gcgatcggtg gcgtcgagga gccccaggat ctcgcggagc
     1681 ccgtcggcca cctcgcggga ggccgtgtcc ttcacgggag ccgcggtggc ggccggcgcg
     1741 cgaggctcgg ccgctggcgg cggctcgaac agctcctcga gaaacggcgc cggcccctcc
     1801 cccatcccca gcttctcgcg cgcggcggcg atcgtctgcc cctcgtcgtt gagcaggcgc
     1861 ttgatctcga ggaccaggtc gatgtcccgc ttgcggtaga gacgctgccc gttgcggctc
     1921 ttcgtcgggg cgagctgcgt gaactccgac tcccagaagc gcagcacgta cggctgcgtg
     1981 tcggtcagct cgcagacttc ctggagcttg tagaagagtt tgcggggaat cgcttcggcc
     2041 atcgggccct cccgctcgcg cggcagacgt cgccccggcg cgcgccggac gcgacgcccc
     2101 gaatgctacg gaaccgggcg gggactgtca aggttcgcgc gtaccggccc gcccatagcg
     2161 atcctcgagg cgcaccacgt cgtcgagctc cggagtcgag acctcgaaca ggatcgcgtc
     2221 ctccagggcc tccatccggt gccgcgtccc ggggggcagg tggatcgcgt cgcccgcctg
     2281 gaggagccgc cgctcgctgc ccgcgctccc ggcggcgtct ccgggcgcgc cgagcgtcag
     2341 ctcgacctgc ccggagaaca ggtacagcgc ctcgtccttg cgctcgtggt actgcaggct
     2401 gagcgactcg cctcgcttca ccgtgagcac cttgccggcg tagcgatcgg tgtgcgcgaa
     2461 gaggatctcc tccccccacg gcttcgggat cctccgaggc gtcattcgcg ctccaggtcc
     2521 cggtgcgacg tgcgcaccag cagcccgcgg gtcccgagat cggacgccac cccctcggcc
     2581 agggcgaccg agcggtgccg accgccggtg cagccaattg ccacggtgag atacgacttc
     2641 ccttcgcgga tgtactgtgg gatgaggaag gtcaggaggt ccacgagccg gcggcgcagg
     2701 tccccgtact cgctcgatcc atcgagatag cgcaccacct cggcgtcgag cccggtccgg
     2761 ccgcggagcc cctccacgaa gaacgggttc gggaggaagc gggtgtcgaa gaccaggtcc
     2821 gcctcgatcg gcagcccgtg cttgaagccg aacgacagga ggatgaccgc catcggcgag
     2881 cccccctcga ggggggcgag gtgctcctgg atgtagcggc gcagctggtg cacgttgaag
     2941 ttgctgctgt cgagcgtgag atcggccagc tcccgcaggg ggcgcagcat ctcccgctcg
     3001 cgggcgatgc cgtcctcgag gctggcgtgc ggcgccagcg ggtgaggccg ccgcgtctcg
     3061 gagaagcgcc ggatgagggt gtcgtcgtcg gcgtcgaaga agatcaggcg cgtccgcccg
     3121 ccggggagcg acttcacgcg cgcgtagatc tccgggaagt ccttcaggag gtccccctcc
     3181 cgcacgtcca cgaccaggac ggc
//
LOCUS       DASRIQ010000603         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1551974, whole genome shotgun sequence.
ACCESSION   DASRIQ010000603 DASRIQ010000000
VERSION     DASRIQ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551974"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFD06_14880"
     CDS             <1..560
                     /locus_tag="VFD06_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HZN04867.1"
                     /translation="DIRITGALQSPAQIPGAVETCNGLDDDCDGRVDEDPSCDDPDDD
                     GVQGIADNCPVVANPDQSDVDQDAVGDVCDLNDGLILITVPALGTVTWQQEAGYETFN
                     WYYGDLAELRESGLYTQQYRRHCNLPMTTVVETDWPCGPGCYPSQPAPGAAVFFLITG
                     VHNGVESSLGTDSSGVPRPNANPCQ"
     gene            658..957
                     /locus_tag="VFD06_14885"
     CDS             658..957
                     /locus_tag="VFD06_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04868.1"
                     /translation="MLDPATGGGTGRHPRGIPGTADDGTITENFDTERDGSPGISLAP
                     LPRGTPGVLNDTIGVWVGTAAGPNAFLVTDVHNGVESSLGTDRSGVPRPNANPCP"
     gene            1132..1566
                     /locus_tag="VFD06_14890"
     CDS             1132..1566
                     /locus_tag="VFD06_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN04869.1"
                     /translation="MRVMVIVKATKNSEAGKMPSQKLLEEMGRFNEELVKAGVMLAGD
                     GLRPSSKGKRVLFEGGKRRIVDGPFAETKELIAGFWIWQVKSMDEALEWVRRCPDPMP
                     GEEGILEIRPFYEAEDFGEEFTPELRAQEDRLRDQMERQRKA"
     gene            complement(1628..1873)
                     /locus_tag="VFD06_14895"
     CDS             complement(1628..1873)
                     /locus_tag="VFD06_14895"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN04870.1"
                     /translation="MTTLTCRDGAALLMDYLEGVLPADTRTAIDAHLSICPRCVEFVR
                     AYRATPRILRESTAFEIPAGLEESLRRFLAGRRGPRS"
     gene            complement(1870..>2196)
                     /locus_tag="VFD06_14900"
     CDS             complement(1870..>2196)
                     /locus_tag="VFD06_14900"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZN04871.1"
                     /translation="RRPTEPLEEYAPRFDADGRHALQEAELALAARADEIIDRQRLAE
                     QARAGIERLPEIYRTAFVLRDLEEMPTAEVAAVLGIDAAAVRQRVHRARLMLRGFLSH
                     LVGVEP"
BASE COUNT          374 a          742 c          772 g          308 t
ORIGIN      
        1 atgacatccg catcacggga gcactgcagt ccccggccca gatccccggc gcagtcgaaa
       61 cctgcaacgg cctggacgac gactgcgacg gccgagtgga cgaagatccc tcgtgcgacg
      121 atccggacga cgacggcgtc cagggtatcg ccgacaactg ccctgtggtt gccaacccgg
      181 atcaatccga cgtcgaccag gacgcggtcg gagacgtctg cgacctgaac gacggtctca
      241 tcctgatcac cgtgcccgcc ctcggcaccg tgacctggca gcaggaagcc ggctacgaga
      301 cgttcaactg gtattacggc gatctcgccg agctgcgcga gtcgggcctt tacacgcagc
      361 agtaccgccg ccactgcaat ctgcccatga ccaccgtcgt cgagaccgat tggccctgtg
      421 gccccgggtg ctacccctct cagccggctc cgggggcggc ggttttcttc ctcatcaccg
      481 gtgtccacaa cggagtcgag agcagcctcg ggaccgacag ctccggcgtc ccgagaccga
      541 acgcgaatcc ctgtcagtaa gggtcgccat gcgcctggct gtcccatcgc tcgcccttgt
      601 tgctgccggc cttctgcttg cggacgacgc gtatgatccc gcggttgccg acccggcgtg
      661 ctcgatcccg ccacgggtgg tgggaccgga cggcatcccc ggggcatccc cgggaccgcc
      721 gacgacggca cgatcaccga aaacttcgac accgagcgcg acggctcgcc ggggatcagc
      781 ctggcgccgc tgccgcgcgg cacgccgggg gtgctgaacg acacaatcgg cgtctgggtc
      841 ggcaccgcgg cgggtccgaa cgccttcctg gtcaccgacg tccacaacgg agtcgagagc
      901 agcctcggga ccgacagatc cggtgtcccg agaccgaacg cgaatccctg tccctagcgc
      961 gatcccgaaa atacgaagga catgccccgc gctcgcccgg cgaccccggc cgtaggaaaa
     1021 tcggtcgggc ctgtcgatcc cgcgccggcc cgttcgtcgt cctgacagga gcaggcaatc
     1081 cggacgatcg aacgaaccgc gccgtgaagc ggcgccgaca aggaggacga gatgcgcgtg
     1141 atggtgatcg tgaaggcgac gaagaactcc gaggcgggca agatgcccag ccagaagctg
     1201 ctcgaggaga tgggcaggtt caacgaggag ctggtgaagg cgggggtcat gctggcgggc
     1261 gacggccttc gccccagcag caaggggaag cgggtgctgt tcgaaggagg caagcggaga
     1321 atcgtcgacg gccccttcgc cgagaccaag gagctgatcg ctggcttctg gatctggcag
     1381 gtcaagtcga tggacgaggc gctcgagtgg gtccgccgct gccccgaccc gatgcccggg
     1441 gaggagggga tcctcgagat ccggccgttc tacgaggcgg aggacttcgg cgaggagttc
     1501 acgccggagt tgagggccca ggaagatcgg ctgcgcgatc agatggaacg gcagcggaag
     1561 gcctgaccga tcgggccgat cgcctggtac gcgcttcagg cgtggcggcg cccggtcgat
     1621 ggaccggtca ggatcgaggc ccgcgccgcc ccgccaggaa ccgccgcagc gattcctcga
     1681 ggccggcggg gatctcgaac gccgtggact cccgcaggat gcggggagtc gcgcggtagg
     1741 cgcgcacgaa ctcgacgcag cgggggcaga tggagaggtg cgcgtcgatg gccgtccgtg
     1801 tgtccgccgg cagaacgcct tcgaggtagt ccatcaggag ggcggcgccg tcgcggcagg
     1861 tcagcgtggt catggctcga ctccgacgag gtggctcagg aaaccgcgca gcatcaggcg
     1921 ggcgcggtgc acgcgctggc gcacggcggc cgcgtcgatg cccagaaccg ccgccacctc
     1981 ggcggtgggc atctcctcca gatctctaag gacgaacgcc gtccggtaga tctcgggcag
     2041 gcgctcgatc cctgcgcgcg cctgctccgc cagccgctgt cgatcgatga tctcgtcggc
     2101 gcgcgcggcc agcgccagct ccgcctcctg cagcgcgtgg cggccgtcgg cgtcgaagcg
     2161 gggcgcgtac tcctcgagcg gctcggtcgg tcgccg
//
LOCUS       DASRIQ010000604         4171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1552182, whole genome shotgun sequence.
ACCESSION   DASRIQ010000604 DASRIQ010000000
VERSION     DASRIQ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552182"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFD06_14905"
     CDS             complement(<1..735)
                     /locus_tag="VFD06_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZN04872.1"
                     /translation="MISSRRRVVVTGIGAVTAAGIGVDPFWRSAVEGRSHLAAANGSI
                     AARFGAMSVGTAPLPPEAQGERAGAAALLATEEALRSARLHDREPLPPETGIVLGTCL
                     GGMAPTLDWIDAMRARGISSEGRGGSRGPDAPAFPSFPVSGGLAGPTRRVAHAFELLG
                     PVVTLSTACSSGAAAIAEACGWIRDGGANLMLAGGAESLTAFVLSGFAILQALTPGRM
                     RPFDRRRDGLALGEGAAIVVLEERRAA"
     gene            complement(745..1545)
                     /locus_tag="VFD06_14910"
     CDS             complement(745..1545)
                     /locus_tag="VFD06_14910"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HZN04873.1"
                     /translation="MSERLRAPIRRLNRCAVGVAAAAFALAAMALAGPAPEAAADPAP
                     EALLRAADAPRRVIDEGAIRIRATVAGPGAAPVVSDLEVLVRGVDQVLCIFRAGPFAG
                     RRILSLRDKVWLIFPGTSRPVPVSASQRLVGGASIADVARLRFAADFDATLRPEREDS
                     DGVPCLVLDLKARDRKVPYASGVLWSGAADRLPRRALFRLRSGLDAKEVRFERYERTA
                     RGLELRREEIVHLLPNERGLTTTLDYLGAEARPIDPALFTPDHAHDPV"
     gene            complement(1538..2764)
                     /locus_tag="VFD06_14915"
     CDS             complement(1538..2764)
                     /locus_tag="VFD06_14915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN04874.1"
                     /translation="MTLWRFAARNIARNRRRTVLTLGVVVFGFAAIALAGGFMAQNLD
                     GLAESTIRSGMGHLQIASPGGFEDAGGFEPGRGLEDAERIAAILRGDAAVKEILPRLE
                     FVGLVSDGRRSVPFLGIGVDPIAEARHMDAPQTIASGRWLADRDERAVVLGGGLAKAL
                     GAKAGDGLTLLATGVDGTLNAVDAAVAGIVHLPIKELDDRWLATGLTLADDLLAAGGR
                     VSRIAVVLGGSRGASAAGRRIEARLREAGLEVEVRPWEALAPFYRQVRILYLGIFGFM
                     GIVLVVVVLLATANTMMMAAAERTREIGTLRAIGTRPSLIRGLFLREGILLAVAGSVL
                     GALLSLLIRTVLNHSGLTLPPPPGGTQGMPLHVQAYAVAYAAAAVAMLLTLAFASWLP
                     ARRASRVPIVEALTHV"
     gene            complement(2761..3459)
                     /locus_tag="VFD06_14920"
     CDS             complement(2761..3459)
                     /locus_tag="VFD06_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN04875.1"
                     /translation="MTAPTPILRAERLTRTYRIGAATVAALRDVDLDLAPGDFLVVQG
                     PSGSGKTTLINLLGLLDRPDAGRVLLEGRPVGDLAEEARADLRRDRIGFVFQTFSLVP
                     VLTAAENVAWPMALRGLGEAETRRKSLALLERVGLAARADVRPDLLSGGERQRVAIAR
                     ALANEPPLVLADEPTAHLDSETAESILDLMAGLNRERGVAFLVATHDPRVVARARRRV
                     ELRDGRFEGEGRAA"
     gene            3537..>4171
                     /locus_tag="VFD06_14925"
     CDS             3537..>4171
                     /locus_tag="VFD06_14925"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZN04876.1"
                     /translation="MESWCRADLHLHTLHSGWRRLHLIDARDSYLTPEAAFVTARRRG
                     MDFVCFTDHDSIDGAMEFLSRHPEEEERVIVGEEREVRLPGTKQWLHLAVYGLNERDH
                     RALARLSDDAFQAIAYLRERQLLFALNHPFQSFRSIGRARRDLLRLLPLVPAVEVANS
                     SSPRSHRIAIEAMLDSWSSGRSSPPARIGGSDAHTTRRIAATFTLAPGPTK"
BASE COUNT          614 a         1591 c         1485 g          481 t
ORIGIN      
        1 cgccgcccgg cgctcctcga ggaccacgat cgccgctccc tctccgagcg ccaggccgtc
       61 ccggcggcga tcgaaggggc gcatccgccc aggcgtcagc gcctggagga tcgcgaagcc
      121 ggacaggacg aaggcggtca gcgattcggc gccgcccgcc agcatgaggt tggccccgcc
      181 gtcgcggatc cagccgcagg cctcggcgat cgccgcggcg ccggaggagc aggcggtcga
      241 cagcgtgacc accggtccga ggagctcgaa ggcgtgcgcc acgcgccggg tcggccccgc
      301 gagacctccg gagaccggga acgaagggaa ggccggcgca tccggccccc gcgagccgcc
      361 gcggccctcg gaggagatac cccgggcccg catcgcatcg atccagtcga gcgtcggggc
      421 catcccaccg aggcaggtgc cgaggacgat cccggtctcc ggggggagcg gctcccgatc
      481 gtggagccgc gcggagcgca gcgcctcctc cgtcgcgagg agcgccgcgg cccccgcccg
      541 ctctccctgc gcctccggcg ggagcggtgc cgtccccacc gacatggcgc cgaagcgggc
      601 ggcgatcgat ccgttggccg cggcgaggtg cgagcgtcct tcgacggcgc tccgccagaa
      661 cggatcgacg ccgatcccgg ccgcggtgac cgcgccgatc ccggtcacca ccacccgccg
      721 gcgtgaggag atcacggagg cggctcagac cgggtcatgg gcatgatccg gggtgaagag
      781 cgcggggtcg atcggccgcg cctcggcgcc gagatagtcg agcgtcgtcg tgaggccccg
      841 ctcgttcggc agcaggtgca cgatctcctc gcggcgcagc tcgaggccgc gcgccgtgcg
      901 ctcgtaacgc tcgaagcgga cctccttggc gtcgagcccg gatcgcagcc ggaacagggc
      961 gcgccgcggc aggcggtcgg cggcgcctga ccacagcacg cccgaggcgt acggcacctt
     1021 gcgatcgcgc gccttcagat cgaggacgag gcacgggacc ccatccgagt cctcccgctc
     1081 cggccggagc gtggcgtcga aatcggccgc gaagcgcagg cgcgcgacgt cggcgatgga
     1141 ggcccccccc accaggcgct ggctggcgct caccggaacc gggcggctcg tcccggggaa
     1201 gatcagccac accttgtccc ggagcgacag gatccgccgc ccggcgaacg gcccggcgcg
     1261 gaagatgcac aaaacctggt ccactccgcg cacgaggacc tcgagatcgg agacaaccgg
     1321 cgcggccccc ggcccggcga ccgtcgcgcg gatccggatc gccccttcgt cgatgacgcg
     1381 ccgcggagcg tccgccgccc gaagcaacgc ctcgggagcg ggatccgccg cggcctccgg
     1441 cgcaggcccg gcgagggcca tcgcggccaa ggcgaatgcc gcggccgcga ccccgacggc
     1501 gcatcggttg agacggcgga tcggcgcccg cagccgctca gacatgcgtc aatgcctcga
     1561 cgatcggcac acgcgaagcg cgtcgcgccg ggagccacga tgcgaatgcc agggtcagga
     1621 gcatcgccac cgccgccgcc gcataagcga cggcgtacgc ctggacatga aggggcatcc
     1681 cctgcgtccc gcccgggggc ggcggcagcg tcaagccgga atggttcagg accgtccgga
     1741 tgagcagcga caggagcgcc ccgaggacgg agccggcgac cgcgagcagg atcccctcgc
     1801 gcaggaacag cccgcggatg agggacggcc gcgtgccgat cgcccgcagc gtgccgatct
     1861 cgcgggtgcg ctccgcggcc gccatcatca tggtgttggc ggtcgcgagc agcacgacga
     1921 cgacgagcac gattcccatg aagccgaaga tcccgagata gaggatacgc acctggcgat
     1981 aaaacggcgc gagcgcctcc cagggacgca cctccacctc gagccccgcc tcgcgcagcc
     2041 gcgcctcgat gcggcgaccc gcggccgaag cgccgcggga gccgccgagg accacggcga
     2101 tgcgcgacac gcgccccccc gccgccagca ggtcgtccgc cagggtgagt ccggtggcga
     2161 gccagcggtc gtccagctcc ttgatcggca ggtggacgat gccggcgacc gcggcgtcga
     2221 ccgcgttgag cgtcccgtcc acgccggtgg cgagcagcgt caacccgtca ccggccttgg
     2281 cgccgagcgc cttcgccagc ccgccgccga ggacgaccgc gcgctcgtcc cgatcggcga
     2341 gccagcggcc cgacgcgatc gtctgcggcg cgtccatgtg acgcgcctcg gcgatcgggt
     2401 ccaccccgat gccgaggaac gggaccgagc gccggccgtc gctcacgagc ccgacgaact
     2461 cgagacgcgg caggatctcc ttcacggcgg cgtctccgcg caggatcgcg gcgatccgct
     2521 ccgcgtcctc gaggccgcgc cccggctcga agccgccggc gtcctcgaag ccgccgggcg
     2581 aggcgatctg gagatgcccc atgccgctcc ggatggtgct ctcggcgagc ccgtcgaggt
     2641 tctgcgccat gaaaccgccg gcgagcgcga tcgccgcaaa gccgaagacg accacgccca
     2701 gcgtcagcac ggtgcggcgc cggttgcgcg cgatgttgcg ggcggcgaac ctccagaggg
     2761 tcacgctgcg cggccttccc cttcgaaccg gccgtcccgg agctccaccc ggcggcgcgc
     2821 ccgggccacg acgcgcggat cgtgcgtggc gacgaggaac gccacgccgc gctcgcggtt
     2881 gagtcccgcc atcaggtcga ggatcgactc cgccgtctcc gagtcgaggt gcgccgtcgg
     2941 ctcgtcggcg aggacgaggg gcggctcgtt ggcgagcgcg cgggcgatcg ccacgcgctg
     3001 gcgctcccct cccgagagca gatcggggcg gacatcggcc ctggccgcga gcccgacccg
     3061 ctccaggagg gccaggctct tcctccgcgt ctccgcctcg cccaggccgc gcagcgccat
     3121 cggccacgcg acgttctccg cggcggtcag caccggcacc aggctgaacg tctggaacac
     3181 gaagccgatc cgatcgcgcc gcagatcggc gcgggcctcc tccgcgaggt cgccgaccgg
     3241 ccggccctcc agcagcacgc gtccggcgtc gggacgatcg agcaggccga gaaggttgat
     3301 caaggtggtc ttcccggacc ccgacggccc ctgcaccacc aggaagtctc cgggggcgag
     3361 gtcgagatcc acgtcgcgca gggcggcgac ggtggcggcg ccgatccggt acgtccgcgt
     3421 cagccgttcg gccctgagga tcggcgtcgg tgccgtcatt ccgctggatt atactttcgc
     3481 gccccgcaga agcggccggt cccggccgcg ggaagcggga tcctcggagg gggaccgtgg
     3541 aatcgtggtg ccgcgccgac ctgcaccttc acacgctcca ttccggctgg cgccgcctgc
     3601 acctgatcga cgcgcgcgac agctacctga ctccggaggc cgccttcgtc accgcgcgcc
     3661 gccgcggcat ggacttcgtc tgcttcactg atcacgactc catcgacggc gcgatggagt
     3721 tcctgtcgcg gcatccggag gaggaggagc gcgtcatcgt cggcgaggag cgcgaggtgc
     3781 ggctccccgg cacgaagcag tggctgcacc tcgcggtcta cggcctgaac gagcgcgatc
     3841 accgggcgct ggcgcgcctc agtgacgacg ccttccaggc gatcgcgtac ctgcgcgagc
     3901 ggcagctcct cttcgccctc aaccatccct tccagagctt ccgttcgatc ggccgcgccc
     3961 gccgcgatct gctgcgcctc ctgccgctcg tcccggcggt cgaggtggcg aacagcagca
     4021 gcccgaggag ccacaggatc gccatcgagg cgatgctcga ctcctggagc tcgggccggt
     4081 cctccccgcc cgcccgcatc ggcggcagtg acgcccacac gacgcggcgc atcgccgcga
     4141 ccttcaccct cgctcccggg cccaccaagg a
//
LOCUS       DASRIQ010000605         7363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3645610, whole genome shotgun sequence.
ACCESSION   DASRIQ010000605 DASRIQ010000000
VERSION     DASRIQ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3645610"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..760
                     /locus_tag="VFD06_14930"
     CDS             107..760
                     /locus_tag="VFD06_14930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN04877.1"
                     /translation="MNPWLRIPIRDYETHMARIGQSEALREIFARACLEARPRRILVL
                     GCTAGGDFPLVDPEVTRLCVGVDLNPEALEEARRRGVERCCEEVRLIQGDLLDVALPD
                     AGFDLIHAGLVLEYVDAARLFERIARWLDPDGTCVTVTQSPVDGIAAVSCTGHDSLHE
                     LSSTMTLRTPEEVKAIARGFGLQRCAARDVPLPGGKLFAVAEFRISTAEPRARRDPR"
     gene            787..1296
                     /locus_tag="VFD06_14935"
     CDS             787..1296
                     /locus_tag="VFD06_14935"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HZN04878.1"
                     /translation="MNIGSYNKVPMAGLRALAERLGMKEVCTLLQSGNLVFESGGAEA
                     ARIERLLEKETKKALGVETDFFVRTAEEWSGVVAANPFKVEEKSDPGHLLLMCLDDAP
                     ARDAVRALEAAIKGRERVKVDGRHAFLVYPDGVGRSRLTTALIESKLGARGTARNWNT
                     VLKLRELLA"
     gene            1293..1895
                     /locus_tag="VFD06_14940"
     CDS             1293..1895
                     /locus_tag="VFD06_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04879.1"
                     /translation="MKVVLGLKAHSGWAALVALGFRGDRPEVVERRRLELIEPGAEWA
                     KAPYHAAEDLKPAEAKRLVERGIAAAHRQSAAAVRGVVKELRDRGLDPVACAVLMGAP
                     MPEWSVAEILAVHFRMHKAEGVLFREALAKAAEAAGLEVVPVPEKELQQIAPRLLGVP
                     WSAITKEVDAFKASVGAPWGKDQKEAALAALIGRRGRRPK"
     gene            2009..2308
                     /locus_tag="VFD06_14945"
     CDS             2009..2308
                     /locus_tag="VFD06_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04880.1"
                     /translation="MNLMTVFIQLASGALGGNIAGALLGRFSLGVLGNSVIGIVGGGL
                     AGQILWYLGAWGRNASAGLDLSAIFWSIAGGVLGGGILILIIGGIRMMVGRATPA"
     gene            2360..3313
                     /locus_tag="VFD06_14950"
     CDS             2360..3313
                     /locus_tag="VFD06_14950"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN04881.1"
                     /translation="MAPPTLSIRRATADDAGRILDCLRAAFEPYRPDYTPAGFLDTVL
                     SPETMRARLTAMTVLVAAEHEAILGTLSYELEGEEGHLRGMAVLPERQGTDVAAALLS
                     HAEADLRRLGCARIRLDTTEPLRRAVRFYLRRGFRPTGRVTDFFGMPLHEYAKRLGAP
                     SKGMDEFRVREATVADLPIVLEHRRRMFEEMGFRDPDDLEGMLAISTDLLRRGLEDGT
                     YRGWFAETPEGKVVAGGGVIVLTFQPHPIDPRPERAWVVNMFTEPEYQRRGLARRLVQ
                     EMIDWCRGQGMRYLYLHASDAGRSLYESVGFIPNNEMRLAL"
     gene            3384..3932
                     /locus_tag="VFD06_14955"
     CDS             3384..3932
                     /locus_tag="VFD06_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04882.1"
                     /translation="MKTIREVGDRPLRWTRPRMLERQYELVSGTDVVGGLRWESMFGS
                     LATAETADGTWTFKRAGFVSPRVTVRTLGSDTDLAVLRVGFKGDGLLHGPDRQTYQWQ
                     RTSFWRSEWAFATETGRLLVGFDPDFSLLKHAATVKLEPEALQNPDLSLLVTLGWYLM
                     LLLSEEVAGVTASTAAAAAAAG"
     gene            3929..4420
                     /locus_tag="VFD06_14960"
     CDS             3929..4420
                     /locus_tag="VFD06_14960"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HZN04883.1"
                     /translation="MIACPGCGLVEPASGRAYDRKFHASAECWALFDQVLAAEFQNAV
                     LFGRAHQMTVDAYAVQHAGGPHPDKSVCVHLAGLCLVFERGVAPVEVPKFLRRIVGVT
                     DRWPHLEPPATHQYGGRTVRDVVRAEDPDRHIATVRAWASEVWEAWRPHHAAARSLVA
                     RAA"
     gene            4417..5025
                     /locus_tag="VFD06_14965"
     CDS             4417..5025
                     /locus_tag="VFD06_14965"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZN04884.1"
                     /translation="MTTGVDATGIGDAVIVTERLDLHPATPILTRAALGPNDLLTTAI
                     DAVVPPSWPPQYLDAAALEFTLAKLAQGPEQAGWWMYFIVLREGDKGRTLIGSGGYVG
                     PPSEDGVVEIGYGIVPDHQRRGYASEAVRGLVRRAFGFPAVTKVTAHTLPELVPSIGV
                     LKKCGFRRTGPGAEEGTIRFELTRDEQRALSGGAEVVRKEEA"
     gene            5022..5324
                     /locus_tag="VFD06_14970"
     CDS             5022..5324
                     /locus_tag="VFD06_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZN04885.1"
                     /translation="MKAYVIVNVDVTDKDRYPEYVKVAPASIQAHGGRYIARGGRTEK
                     LEGTAWEPKRVVLLEFPSFEQAKAWWDSQMYAEPKKLRQACARTEMILIEGCPPVF"
     gene            5324..5935
                     /locus_tag="VFD06_14975"
     CDS             5324..5935
                     /locus_tag="VFD06_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HZN04886.1"
                     /translation="MEFWFEFASTYSYLAAMRVEKVAAARGVVVAWRPFLLGPLFQKQ
                     GWNDSPYNLNPARGRYMWRDIERATHRLGIPFRKPSVFPRRSILPARLACLGHGTAWL
                     PEFARAVFRANFADDREIEDPVLMRGLLRQAGAAADEAGAAELLERATSQGTKDLLRS
                     ETDRAWSLGIFGAPTFVVGGELFWGNDRLEEALDWYTRAHGRP"
     gene            5944..6657
                     /locus_tag="VFD06_14980"
     CDS             5944..6657
                     /locus_tag="VFD06_14980"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZN04887.1"
                     /translation="MQTPGVPLPILFEDDDLYAVDKPEGLPVIPERIARGPCAVEMAA
                     AQAGGRLFVVHRLDRDTSGVLLFARNAAAHRHLNGEFEHRRARKTYAAVAEGRMVTRD
                     GKVTMPIRPFGSGRMGIDRRRGKPCLTHYRLVRFAGGCTELLAHPVTGRRHQIRVHLY
                     CLGHPIVGDRRYGPPPEGGPAGPASRSGGPGRVEAERLMLHARSLAVRLPSGGAVRVT
                     SPLPATYLAILARLRADGG"
     gene            6722..7123
                     /locus_tag="VFD06_14985"
     CDS             6722..7123
                     /locus_tag="VFD06_14985"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZN04888.1"
                     /translation="MTRDGGCFCGALRYTVTGEPRNASHCHCLHCRRTSAAAFVTWAT
                     FPSDAFVWMHGQPASFASRPGVTRTFCNACGTPLTFESADDPGAIDITICSLDDPASI
                     TPRDHVFASRMLSWADLKDGLHRFALSRRDG"
     gene            7132..>7363
                     /locus_tag="VFD06_14990"
     CDS             7132..>7363
                     /locus_tag="VFD06_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN04889.1"
                     /translation="MSGPGKPRIGAITWIDLTVGAARDVRDFYQDVVGWTAGDVEMGG
                     YSDFTMHPPGGGEPVAGVCHARGPNAELPAHWL"
BASE COUNT         1048 a         2499 c         2726 g         1090 t
ORIGIN      
        1 cgcttcgcgc tgaatacgga cgggatcacg ccgcatgacg cggtcctctt ctacaaggac
       61 ctcgaccaac tgccgtgagc gcggaggaga gtctggaggc ggcggaatga atccctggct
      121 gcggatcccg atccgggact acgaaacgca catggcccgg atcggacagt cggaggcgct
      181 gcgcgagatc ttcgcgcgcg cctgcctgga agcccggccg cggcgcattc tcgtcctcgg
      241 gtgcacggcc ggtggcgatt tcccgctcgt cgatcccgag gtcacgcggc tctgcgtcgg
      301 cgtcgatctg aacccggagg cgctcgagga ggcgcgtcgg cggggggtgg agcgctgctg
      361 cgaggaggtc cggctgatcc agggggacct cctcgacgtg gcgctcccgg acgcggggtt
      421 cgacctgatc cacgccgggc tcgtcctcga atacgtcgat gccgcgaggc tgttcgagcg
      481 catcgcgcga tggctcgacc ccgacggcac ctgcgtcacg gtcacgcaga gccccgtcga
      541 tgggatcgcc gccgtctcct gcaccggcca cgacagcctg cacgaactct cctccaccat
      601 gacattgcgg acgccggagg aggtgaaggc gatcgcccgg ggcttcgggc tgcagcgctg
      661 cgccgcccgc gacgtgccgc tgcccggcgg gaagctgttc gcggtggcgg aattcaggat
      721 ctccacggcg gagcctcggg ctcggaggga tccgcgatga cgagatatgt ggcgctcttg
      781 cgcgcagtga atatcggctc ctacaacaag gtcccgatgg cgggcctgcg ggcgctggcg
      841 gagcgcctcg gtatgaaaga ggtctgcacg ctgctgcaga gcggcaacct cgtcttcgag
      901 agcggcggtg cggaggcggc gcgcatcgag cgcctgctgg aaaaggagac gaagaaggcg
      961 ctcggtgtcg agaccgattt cttcgtccgg acggcggagg agtggagcgg cgtggtcgcg
     1021 gccaaccctt tcaaggtgga ggagaagtcg gaccccggcc acctgctcct gatgtgtctc
     1081 gacgatgccc ccgcgcgcga cgccgtgcgc gctctcgaag cggcgatcaa ggggcgcgag
     1141 cgggtgaagg tggacggtcg ccacgccttc ctcgtctacc ccgacggcgt cggccgctcg
     1201 cgcctgacga ccgccctcat cgagtcgaag ctgggagcgc gcggcacggc gcgcaactgg
     1261 aacaccgtgc tgaagctcag ggagctgctc gcatgaaggt cgtcctcggt ctcaaggcgc
     1321 actcgggctg ggcggcgctc gtcgcgctgg gattccgcgg cgatcggccg gaggtggtcg
     1381 agcgccgccg gctcgagctc atcgagccgg gcgcggaatg ggccaaggcc ccctaccacg
     1441 ccgccgagga cctgaagccg gcggaggcga agcggttggt cgagcgcggg atcgccgcgg
     1501 cgcaccgcca gtcggccgcc gccgtgcggg gcgtggtgaa ggagctgcgc gatcgcgggc
     1561 tcgatccggt ggcgtgcgcg gtcctcatgg gggcgccgat gccggagtgg agcgtcgcgg
     1621 agatcctcgc ggtgcacttc cgcatgcaca aggcggaagg ggtgctgttc cgcgaggcgc
     1681 tcgcgaaggc cgccgaggcg gcggggctcg aggtcgtgcc ggtgccggag aaggagctgc
     1741 agcagatcgc gccgcgcctc ctcggcgtcc cctggtccgc catcacgaaa gaggtggacg
     1801 ctttcaaggc gtcggtcggc gccccgtggg gaaaggacca gaaggaggcc gcgctcgccg
     1861 ccctcatcgg ccggcgcggc cgccgtccga agtagccggc cgccggctcc gtccgtccgg
     1921 aggagtgccg acggcggtcc tgcgtcgtga tccgcatgcg gcctcgttct tttgactttc
     1981 ccgcgtccgc gccgcaacat gcgacaccat gaatctgatg acagtcttca tccagctggc
     2041 gagcggcgcg ctcggcggca acatcgccgg ggcgctcctc ggccggttca gtctcggcgt
     2101 gctcggcaac tcggtgatcg gaatcgtcgg cggcggattg gcggggcaga tcctctggta
     2161 cctcggggcc tggggacgca acgcgtcggc cgggctcgat ctgagcgcga tcttctggag
     2221 catcgccggc ggcgtcctgg gcggcggcat cctgattctc atcatcggcg gcatccgcat
     2281 gatggtcggc cgggccacgc cggcctgagc tcgcgccgaa ccgcggccgt cctatactcc
     2341 ccggcacacc ggagccgcga tggcacctcc gactctttcg atccgccgcg ccacggccga
     2401 cgacgccggc cgcatcctcg actgcctgcg cgcggccttc gagccgtatc gccccgacta
     2461 cactcccgcc gggttcctcg acacggtgct gagcccggag acgatgcgcg ccaggctcac
     2521 ggcgatgacg gtcctcgtcg ccgccgagca cgaggcgatc ctcggcacgc tgtcgtacga
     2581 gctcgaaggg gaggaagggc acctgcgcgg catggcggtc ctgccggagc ggcaggggac
     2641 agacgtcgcg gcggcgctcc tgagccacgc cgaggccgac ctgcgccgtc tcggctgcgc
     2701 ccgcatccgg ctcgacacca ccgagccgct ccgacgcgcg gtccggttct atctccggcg
     2761 cggcttccgg ccgaccggac gcgtgaccga tttcttcggg atgccgctcc acgagtacgc
     2821 caagcggctc ggcgcgccgt cgaaggggat ggacgagttc cgcgtgcgcg aggcgaccgt
     2881 cgccgacctg ccgatcgtgc tggaacaccg gcggcggatg ttcgaggaga tgggcttccg
     2941 ggatcccgat gacctcgaag ggatgctggc gatctccacc gatctcctga ggcgcggcct
     3001 cgaggacggc acctatcgcg gctggttcgc ggagacgccg gaggggaaag tcgtcgccgg
     3061 aggcggcgtc atcgtcctca ccttccagcc ccatccgatc gacccgcgcc ccgagcgggc
     3121 ctgggtcgtg aacatgttca ccgagccgga gtaccagcgc cgcgggctgg cgcggcgact
     3181 ggtgcaggag atgatcgact ggtgccgcgg gcaggggatg cggtatctgt atctgcacgc
     3241 cagcgacgcc ggccgctccc tctacgagag cgtgggattc atccccaaca acgagatgcg
     3301 gctggcgctg tgacctcggc gctagaatga gggctcaccc ggcaggctgg accaggcagg
     3361 accaggcagg acgggaggca gggatgaaga cgattcggga ggtgggcgac cgccccctcc
     3421 gctggacgcg ccccagaatg ctggagcggc agtacgagct ggtgtcgggg acggatgtcg
     3481 tcggcgggct ccgctgggag agcatgttcg ggtcgctcgc cacggcggag acggcggacg
     3541 ggacctggac gttcaaacgg gccggcttcg tgagcccgcg cgtgacggtc cgcacgctcg
     3601 ggtccgacac cgacctcgcc gtgctgcgcg tcggcttcaa gggggacggc ctgctgcacg
     3661 ggcccgaccg ccagacctac cagtggcagc ggacgtcctt ctggcgttcc gaatgggctt
     3721 tcgcgaccga gaccgggcgt ctcctggtcg ggttcgatcc cgatttctcc ctgctgaagc
     3781 acgcggccac ggtgaagctc gagccggagg cgctacagaa cccggacctg tcgctcctgg
     3841 tgacgctcgg ctggtacttg atgctgctgc tctcggagga ggtcgccggt gtcactgcct
     3901 cgaccgccgc cgccgcggcc gcggcaggat gatcgcctgt ccggggtgcg ggctggtgga
     3961 gccggcgagc gggcgcgcct acgatcggaa gttccacgcc tcggcggagt gctgggccct
     4021 cttcgaccag gtcctcgccg cggagttcca gaacgcggtg ctgttcgggc gggcgcacca
     4081 gatgacggtg gacgcctacg cggtgcagca cgccggcggg ccgcatccgg acaaatcggt
     4141 gtgcgtgcat ctcgccggcc tctgcctcgt cttcgagcgc ggggtcgctc cggtcgaggt
     4201 gccgaagttc ctgcgacgga tcgtcggcgt aaccgatcgc tggccgcacc tcgagccgcc
     4261 tgcgacgcac cagtacggcg gaagaacggt tcgagacgtg gttcgcgccg aggatccgga
     4321 tcgccacatt gcaacggtgc gcgcctgggc gtccgaagtc tgggaggcct ggcgtccgca
     4381 tcacgccgcc gcccgctcgc tcgtggcccg ggccgcatga cgaccggcgt ggacgcgacc
     4441 gggatcgggg acgcggtgat cgtgaccgag cggctggacc tccatccggc cacgccgatc
     4501 ctcacgcggg ccgcactcgg cccgaacgac ctcctcacca cggcgatcga cgccgtggtg
     4561 ccgccgtcgt ggccgccgca gtatctcgac gccgcggcgc tggagttcac cctggcgaag
     4621 ctggcgcagg ggccggagca ggccggctgg tggatgtact tcatcgtcct gcgcgagggg
     4681 gacaaggggc ggacgctcat cggcagcggc gggtacgtcg ggccgccctc tgaggacggc
     4741 gtggtcgaga tcggctacgg gatcgtcccc gatcaccagc ggcgcggcta cgcctcggag
     4801 gcggtgcggg gtctcgtgcg gcgcgccttc gggttccccg ccgtcacgaa ggtcacggcg
     4861 cacacgctgc ccgagctggt gccgtcgatc ggcgtcctga agaaatgcgg cttccggcgg
     4921 accggccccg gcgccgagga ggggacgatc cgcttcgagc tgacgcgtga cgagcagcgg
     4981 gcgttgtccg gcggcgcgga ggtggtccga aaggaggagg cgtgaaggcg tacgtcatcg
     5041 tcaacgtcga tgtgaccgac aaggaccggt atccggagta cgtcaaggtg gccccggcgt
     5101 cgatccaggc acacggcggc cgctacatcg cgcgcggcgg ccgcacggaa aaactggaag
     5161 ggacggcgtg ggagccgaag cgggtggtgc tgctcgagtt cccgtccttc gagcaggcga
     5221 aggcatggtg ggactcgcag atgtacgcgg agccgaagaa gctgcggcag gcgtgcgccc
     5281 gcaccgagat gatcctgatc gaaggctgcc ctcccgtctt ctgatggagt tctggttcga
     5341 gttcgccagc acctattcgt acctcgcggc gatgcgggtg gagaaggtgg ccgcggcacg
     5401 aggtgtcgtt gtggcgtggc ggccgttcct cctggggccg ctgttccaga agcagggctg
     5461 gaacgacagc ccgtacaacc tgaacccggc gcgcgggcgc tacatgtggc gcgacatcga
     5521 gcgcgccacg caccgtcttg gaattccgtt ccggaagccc tcggtcttcc cgcgccgatc
     5581 gatcctgccg gcgcggctcg cctgtctcgg acacgggacg gcgtggctgc cggagttcgc
     5641 gcgcgcggtg ttccgggcga acttcgccga cgaccgggag atcgaggatc ccgttctaat
     5701 gcgcggcctg ctgcgccagg ccggggcggc cgcggacgag gccggagccg cggagcttct
     5761 ggagcgcgcg acatcgcagg ggacgaagga tctgctgcgc tccgagaccg accgcgcctg
     5821 gtcgctcgga atcttcggcg cacccacctt cgtcgtcggg ggggagctgt tctgggggaa
     5881 cgaccggctc gaggaggcgc tcgactggta cacccgcgcg cacgggcgcc cctgatcgcc
     5941 ggcgtgcaga cccccggcgt gcccctgccg atcctgttcg aggacgacga tctgtacgcc
     6001 gtggacaagc cggaggggct gccggtcatc ccggagcgga tcgcgcgcgg gccgtgcgcg
     6061 gtcgagatgg ccgcggcaca ggccggcggg cggctcttcg tcgtgcaccg gctggaccgc
     6121 gacaccagcg gcgtgctcct cttcgcgagg aacgccgcgg cacaccgcca cctgaacggt
     6181 gaattcgagc accgccgcgc gcgcaagact tacgccgcgg tggccgaggg gcggatggtg
     6241 acgcgggacg gcaaggtcac gatgccgatc cggccgttcg ggtcggggcg catggggatc
     6301 gatcgccgcc gcggcaagcc ctgcctgacg cactaccggc tcgtccggtt cgcgggaggc
     6361 tgcacggagc tgctcgccca tccggtcacc ggccggcgtc accagatccg cgtgcacctc
     6421 tactgcctcg gtcatccgat cgtcggcgac cggcgctacg gcccgcctcc cgaaggcggt
     6481 cctgcggggc cggcatcccg gtcggggggg ccggggcgcg tggaggcgga gcggctgatg
     6541 ctgcacgccc gctcgctcgc ggtccgcctc ccgtccggag gcgcggttcg agtgacgtcg
     6601 ccgctgccgg ccacgtacct cgcgattctc gcgcggctgc gcgccgacgg tggataatgc
     6661 gcccctgcgc ccacccccgc gcagggccgg gcggattcgg cctggcaagg agaaagagac
     6721 catgacacgc gacggcggtt gcttctgcgg cgccctgcgc tacacggtca ccggcgagcc
     6781 gaggaatgcc tcgcactgcc actgcctgca ctgccgccgc accagcgcgg cggcgttcgt
     6841 cacctgggcg accttccctt cggacgcgtt cgtctggatg cacgggcagc cggcgtcgtt
     6901 cgcgtcgcgt ccgggggtga cgcgcacctt ctgcaacgcg tgcggcacgc cgctgacctt
     6961 cgagtcggcg gacgatcctg gcgccatcga catcacgatc tgcagcctcg acgatccggc
     7021 gagcatcacg ccgcgggacc acgtcttcgc ctcgcgcatg ctgtcgtggg ccgatctcaa
     7081 ggacgggctg caccgcttcg ccttgtcgcg gcgggacgga taggaggagg aatgagcggg
     7141 ccggggaaac cgaggatcgg cgccatcaca tggatcgacc tgacggtcgg cgcggcgcgc
     7201 gacgtgcgcg acttctacca ggacgtggtc ggctggaccg ccggcgacgt ggagatgggg
     7261 gggtactcgg acttcaccat gcaccctccc ggcggcggcg agccggtggc cggcgtctgc
     7321 catgcgcgcg gcccgaacgc cgaactgccg gcgcactggc tca
//
LOCUS       DASRIQ010000606        11746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1301635, whole genome shotgun sequence.
ACCESSION   DASRIQ010000606 DASRIQ010000000
VERSION     DASRIQ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 11746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11746
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301635"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="VFD06_14995"
     CDS             <1..1399
                     /locus_tag="VFD06_14995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN04890.1"
                     /translation="LTWTATEHQGRKAIFMTVADLGARARAERSIAASEARLRAALDA
                     AGDPLALLHEKAGGGFVVAQANRAFKALLPLSGPAPAGTNALDLLGRLASDPAGAEAF
                     ASLLAEARQGSEARREGIDLRGAGRALLVDAAASPVRAPGGGTEPFGLVLTLRDVTVR
                     AGQERALRGSVDDLTATTRRLEESLRELAEARASLQERNAQLERLNGELRSLDEMKSN
                     LLANVSHELHTPLVSIKGYTEMILKRKLGPLTPEQERGLLVAQKNIDRLIELIDNLLS
                     FARLETGETQLRIEDVPLHQLVDEAIELVSERVKRRNLSVTTQYEGDDLVVRGDRVKI
                     GQVFTNLLTNAVKFNREGGRIAVAARRGRGGFVEVEVADTGIGIPPEEQEKIFERFYQ
                     VEAGPRRRYEGTGIGLAIVRDILRLHGGSIRVQSTPGQGSTFQFTLPLGRKPEGNSAR
                     PAGGRNRTDEQAPGS"
     gene            complement(1406..2239)
                     /locus_tag="VFD06_15000"
     CDS             complement(1406..2239)
                     /locus_tag="VFD06_15000"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZN04891.1"
                     /translation="MSGRGSSFAVVIRSAQHPALKDARRLEADAGARRKEGRAIAWGR
                     HLALEALAAGVPIERALLAPDLEDDAEGGRIAAAIEEAGVPALRVAAALLDGIAAGAG
                     DQRVVLVLRRRAPTLAELLQRRPGLLLAAHGVQDPGNLGSMIRSAHALGADGVLTFEG
                     SADPWSSRALRAAMGAAFRLPIVETESAAALEALRGAGLRIVAADAAAGSPPAAVDLR
                     PPAVILVGREGAGLPRPLLDAAETKVGIPMQPGVDSLNVHAAAAILLYEARRQRDEAG
                     R"
     gene            complement(2236..4479)
                     /locus_tag="VFD06_15005"
     CDS             complement(2236..4479)
                     /locus_tag="VFD06_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN04892.1"
                     /translation="MIAALGVLAFAGCGRRPADPDGITVIVDSGPNSLDPRLGSDEAS
                     RRVFDLLFNSLFRIADDGDVVPDLAESAASPDERTLVVRLREGVRFHDGALLTSRDVA
                     RTYRSILDGEVVSFRRADFESVESVEAPDPRTVVFRLRRPFAPLLSNLTVPILRDAPA
                     SGEGGAPIGTGPFRLQRYRKDEDLLLTRFDAYFEGASPLTSVRIRILPAESARLLEML
                     RGSGDLVVNDLAPEHFDRLGRTAGFEVAAHDGRNAVYLGFNLTRPPLDDVRVRRAIAL
                     AIDREAIVTHLLSGRARLATALLPPGHWAHDDGVPVRGADAAEARRLLDDAGFADPDG
                     DGPQSRLRLRYSAPQSEQAAQQASVLQEQLAQVGIALDVFTFEWPTFYDDLRSGRFEI
                     LTSNWTDVGDPDIYRLRFHSAFRPPAGLNRGGYASPEADRLIETGATLSDRSERLAAY
                     AALQRLLDRDLPCVWLWHRQVRAASGPRLRGFALGAGADFRPLWRARVVASGEAAAER
                     RLDRERRDRSRAHEAGRLDREIDDGRGGAAVRAPGVEDRRDAPPEMLLDLGGGGGGRL
                     AAPVGARGDERPAAGSRQRQGDRVRGDAHADRRSVSEQARRQAVRRPQDEREGAGPED
                     VHQGGRPVGKRRRDASDLLPMAQDHREGHPLRPSLGREDSLDRLRIQGVGAERVEGFR
                     GEGDQAAALQEIHRRGQAFRFGPPRIDRLDPRGHRSHPPPGGAAGHYSAGALPIGEPW
                     PARESSA"
     gene            4609..6897
                     /locus_tag="VFD06_15010"
     CDS             4609..6897
                     /locus_tag="VFD06_15010"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN04893.1"
                     /translation="MHLRHLEPAPGGRRRARRAAVAVIVLLLAGGIGAAAENRFERVI
                     VLGFDGADARLVSQYMDEGRLPNLARLRDQGTYAPLRSTNPPQTPVSWATFATGLNPG
                     RTEIFDFLRRVEGTYLPDFAMVREGKRPFLLGPKNPMVVAPIAFAVVGLLAAVGLWLA
                     GARGLRLALGALVAGALAGGGAAFLAARWLPESVPDATNGRRGLPFWTAAAAAGIPSR
                     VIHVPANFPAESHDHLELLSGLGVPDMRGRIGSPSLFTSDAALAVPDNQFSVEIVHLS
                     GRRGRIETALAGPLNDPFWNYPIAKATEGLTGDEKQATRARVEETLKARGVRRRLDVP
                     FVIDATGSDLTIDLGGRRETLKPLGWSDWFVVPVAVNPLVDRVAGLRGMVRFKLLALE
                     PEIRLYMSPLMFHPKCQPVPFATPAGFAGELLDEVGLFKTLGWPIDTWSLPSGIADEQ
                     HFLEDLEFTVATEVKMLDQALERGDARLYVQVFDFTDRIGHLFWRYHDPGHPIYDAAA
                     AGRYEPEIPRAYERMDAIVGRAMARLDGKTALLVCSDHGFASFRRGVNYNTWLVRNGF
                     MTLKGAMGGGGKTLEDLFDKGEMGGFFKNVDWSRTKAYAMGLGNIYLNLLGREPKGSI
                     APGREYEEVRDAIIAGLEALVDPKTGEKPVTKVYKREEIYSGFDPRVVPDLRVANSEH
                     YRIGWQSTLGEVPLEIFEDNKKAWSGDHCSNDPAIVPGILFSSVRLQKEREPFIGDLF
                     PTTLALLGVPAVPDIDGRSLLP"
     gene            6903..8147
                     /locus_tag="VFD06_15015"
     CDS             6903..8147
                     /locus_tag="VFD06_15015"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZN04894.1"
                     /translation="MIARRPVRPARRTTPSAAALLVGAALLGPALHGAEPSPAPAESG
                     TGRPKDDSDARRLAALQAQIEALRARLVAAEEQAGTILDDLDEMGLRLSLLARESEML
                     QREIDAARVQVMRSRREEEAARERLTKAEQDLRDWILELYKSGPPPDLSLMLLAGSPA
                     EVAGAQRSAEALAVAEGRRVEAVRSERERLAAALAQRERQEERLLDLQKELEQRRADL
                     GTAKAKKGRLLDDIRTRQESEEEALEGMVQMERDLTALLGRVQDPASPSRGLARFHGL
                     LAWPAAGSVALPFGNVRHPRFHTQVPHPGLEIACDPGTEVRALFVGRVVYSDWLRGYG
                     EMIVIDHGDEYLSIYGQLGERLVAVGQEVRQNQPIARSGTEGTFGVTGLYLEIRHRGE
                     PEDPLPWLRQSGRRPVSRERKK"
     gene            8144..9436
                     /locus_tag="VFD06_15020"
     CDS             8144..9436
                     /locus_tag="VFD06_15020"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZN04895.1"
                     /translation="MTLSLDRKVLVVLSALVVVLTLAGGFAGRTGAVEGTYDYLKVFN
                     EVLYLSVNNYVEPAQIEALMDGAYRGMLETLDPGNEYLTQREYDRASRGESGGPAEVG
                     LRLSKRHGYIVVISSLPGSPAAAAGLKTGDAILTIDSRPTRLMGVWEAEQALRGKTGS
                     KVTLGLSPSEGGERREQALERRTITPPAPSGRLAEEGVGTVRLLGLRDGDAKRVDQAI
                     ASLKEQGMKRLLLDLRGCVSTSLAEGIGVASLFMKDGVVVTVVDRHDGDKAYRADGRK
                     RSWSGPMVVLVDEGTSRTCEVVTAALRDGLGTPVVGERTWGAGTVMSLLPLPNGDGLF
                     LATGLMQSPAGKEWNAKGLDPDLAIPSDTEAGKDTQRQKAIDYLKGVTRQTASLSSSV
                     PGTPVARNRAGATARPARPENDGTYIARGLPGAHREAV"
     gene            9641..11128
                     /locus_tag="VFD06_15025"
     CDS             9641..11128
                     /locus_tag="VFD06_15025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN04896.1"
                     /translation="MSIPKNIGRYEIVEEVGRGSMGVVYRARDPRLGRIVALKCVAFS
                     FPLGPGEEEEFLRRFYLEAQIAGRLSHPNIVTIHDVGTRETEGTAFIAMEYVTGTNLQ
                     ELLAGGGRLPIPQVADVARQMADALDYAHGCGVVHRDIKPGNIVLTTGGQVKVLDFGI
                     ARAPSGDSTKPGRMLGTPNYMAPEQVTGATVDGRADQFSLSVTLYHLLTGERPFVGES
                     LTAISYQVVNVTPPPPSRLNPALPAEVDRILARGLSKNAPDRYPGCGDLASEFGAALA
                     TWKEAVDRAVPRTLVAATDASLRAGVSTGPSAGRGLAGVLSGAGPGAVAGWTLFLGAL
                     GALALSPYLLNAVTREDRTGGPAQRPRPGASQVAPPSWLRLPPAPSPAPPRAAKPAPV
                     ARGTLLLTFQHRFASGMVTVRVDGAEAVRERISASGRTTWTKSIPLAAGRRRVEARIE
                     ANRGDVDEIAGIDLEIREHRTRTIVLSMNALTRRLKIRPEEDQPR"
     gene            11125..>11746
                     /locus_tag="VFD06_15030"
     CDS             11125..>11746
                     /locus_tag="VFD06_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04897.1"
                     /translation="MRARRRSALLASCLALLAAGAAVADDRPLIGPRARTYAVFLEAQ
                     NLLLAGRADEAARLLDAAVSADPDPGLLLEAARMHELLGEPDQALPLADRALLARPGW
                     PEALVLRAGLRLDRDDEPAPEHRLASLADLRVALATDPSSEEGARRLAAICAGSDCAG
                     EAARVLEAQRANRVLPVDLALALARLDLEIGKVEECRAILTEILQRD"
BASE COUNT         1694 a         4231 c         4233 g         1588 t
ORIGIN      
        1 cctcacctgg accgcgaccg agcaccaggg gcgcaaggcg atcttcatga cggtggccga
       61 cctgggcgcc cgcgcccgcg ccgagcggtc gatcgccgcg agcgaagcgc ggctgcgcgc
      121 cgccctcgac gcggccgggg atcccctggc gctcctccat gagaaggccg gcggcggctt
      181 cgtcgtggcg caggcgaacc gggcgttcaa ggcgctcctt cctctgtccg gccccgcgcc
      241 ggcggggacc aacgcgctcg acctgctcgg ccggctcgcc tccgatcccg cgggagcgga
      301 ggcgttcgcc tcgctcctgg ccgaggcgcg gcaggggagc gaggcgcgcc gcgaaggaat
      361 cgacctgcgc ggcgccggcc gtgccctcct cgtggacgcg gccgcctccc cggtgcgcgc
      421 cccggggggc ggcaccgagc cgttcgggct cgtgctgacg ctgcgcgacg tcacggtgcg
      481 cgccggtcag gagcgggcgc tgcgcggcag cgtcgacgat ctgaccgcca caacgcggcg
      541 gctcgaggag tcgctgcgcg agctggccga ggcgcgcgcc tcgctccagg agcgcaacgc
      601 ccagctggag cggctcaacg gcgagctacg ctccctcgac gagatgaagt cgaacctgct
      661 cgccaacgtg tcgcacgagc tgcacacgcc cctcgtgtcg atcaaggggt acaccgagat
      721 gatcctgaag cggaagctcg gtcccctgac gccggagcag gagcgcggcc tgctcgtcgc
      781 gcagaagaac atcgaccgcc tcatcgagct gatcgacaac ctgctctcct tcgcccggct
      841 cgagaccggc gagacgcagc tgcgcatcga ggacgtgccc ctccaccagc tggtggacga
      901 ggcgatcgag ctggtctcgg agcgggtgaa gcggcgcaac ctctcggtga ccacccaata
      961 cgagggggac gatctcgtgg tgcgcggcga ccgggtgaag atcggccagg tcttcacgaa
     1021 cctcctcacc aacgcggtga agttcaaccg ggaaggagga cgcatcgccg tggcggcgcg
     1081 tcgcggccgc ggcggcttcg tcgaggtgga ggtggccgac accggcatcg gcatcccgcc
     1141 ggaggagcag gagaagattt tcgagcgctt ctaccaggtg gaggcgggac cgcggcgccg
     1201 ctacgagggg accggcatcg gcctggcgat cgtgcgcgac atcctgcgcc tgcacggcgg
     1261 cagcatccgc gtgcagagca ctcccggcca gggatcgacc ttccagttca ccctgcccct
     1321 cggccgcaag ccggagggga actccgcccg tcccgccggt ggccgcaacc gaaccgacga
     1381 acaggcgcca ggctcctagg ctcctctagc gccccgcctc gtcgcgctgt cgccgcgcct
     1441 cgtaaagaag gatggcggcc gcagcgtgca cgttgaggct atcgaccccc ggctgcatgg
     1501 ggatgccgac tttcgtctcc gctgcgtcga gcagcgggcg cggcaggccg gccccctccc
     1561 ggccgacgag gatcaccgcg ggaggacgca gatcgaccgc cgccggggga gaccctgcgg
     1621 ccgcgtcggc cgccacgatc cgcagccccg ccccccgcag cgcctcgagg gccgcggcgc
     1681 tctccgtctc cacgatcggc agccggaacg cggctcccat cgcggcgcgc agggcgcggc
     1741 tcgaccaggg atcggcgctc ccctcgaacg tcaggacgcc gtccgccccg agcgcatgtg
     1801 ccgagcggat catcgagccg aggttgccgg ggtcctgcac tccgtgcgcc gcgaggagga
     1861 gtccgggccg gcgctggagc aactcggcga gggtcggggc gcgtcggcgg agcaccagca
     1921 cgacccgctg atctcctgcg cccgcggcga tgccgtcgag cagggcggcc gccacgcgca
     1981 gcgcgggcac gcccgcctct tcgatcgccg cggcgatccg gcccccctcc gcatcgtcct
     2041 cgagatcggg cgcgagcagg gcccgctcga tggggacgcc ggcggccagc gcctcgagcg
     2101 ccaggtgccg tccccacgcg atcgcccgcc cctccttcct ccgcgccccg gcatccgcct
     2161 cgagccgtcg cgcgtccttg agagccggat gctgcgccga acggatcacc acggcgaagg
     2221 aagaaccgcg cccgctcatg cggaggactc ccgcgcgggc cagggctccc cgatcggaag
     2281 cgcgcctgcg ctatagtggc ccgccgcccc gccgggtgga ggatgcgagc gatgacctcg
     2341 aggatcgagc cggtcgatcc gcggcggccc gaaccggaac gcttggccgc ggcggtggat
     2401 ctcctgaagg gcggcagcct ggtcgccttc cccacggaaa ccttctacgc gctcggcgcc
     2461 gacgccctga atccggaggc ggtccagcga gtcttcgcgg ccaagggacg gccgcagggg
     2521 atgcccctcc cggtgatcct gggccatcgg gaggaggtcg ctcgcgtcgc ggcggcgctt
     2581 cccgacgggg cggccgccct gatggacgtc ttctggcccg gccccctcac gctcgtcctg
     2641 cgggcggcgg acggcctgcc gccgcgcctg ctcggagacg gatcgacgat cggcgtgcgc
     2701 gtctccccgc acccggtcgc cttggcgctg gcgcgagcct gcggccggcc gctcgtcgcc
     2761 acgagcgcca accggagcgg ccagccgccc cccgccaccg ccgccgagat cgaggagcat
     2821 ctcgggggga gcgtcgcggc gatcctcgac gccgggcgca cgcacggcgg ctccccctcg
     2881 accatcgtcg atctcacggt cgagccgccc cgcctcgtgc gcgcgggagc gatctcgtcg
     2941 ctcgcgatcg agtcggcgct cggccgccgc ctcgcctgac gcgacgacgc gcgcccgcca
     3001 cagcggccgg aagtcggccc ccgcgccgag cgcgaagccg cgcagccgcg gcccggaggc
     3061 ggcgcgcacc tgccggtgcc acagccagac gcacgggagg tcgcgatcga ggagccgctg
     3121 cagtgccgcg taagccgcga gacgctccga tcgatcggag agcgtcgctc ccgtctcgat
     3181 gagccgatcg gcctcgggac tcgcgtagcc gccgcggttg agcccggcgg gagggcggaa
     3241 ggccgaatgg aagcgcagcc ggtagatgtc cggatcgccg acgtcggtcc agttggaggt
     3301 gaggatctcg aaccgaccgg agcgcaggtc gtcgtagaac gtcggccact cgaaggtgaa
     3361 gacatcgagc gcgatcccga cctgggccag ctgctcctgc aggaccgacg cctgctgcgc
     3421 cgcctgctcg ctctgcgggg cgctgtagcg gaggcgcaga cgggactgcg ggccgtcccc
     3481 gtccgggtcg gcgaaaccgg cgtcgtcgag caggcgccgc gcctcggccg cgtcggcgcc
     3541 gcggaccggc accccgtcgt cgtgggccca gtgtccgggc gggaggagcg ccgtcgccag
     3601 ccgcgcccgg ccggagagga ggtgggtcac gatcgcctca cgatcgatcg ccagggcgat
     3661 cgcccgccgc acacgcacgt cgtcgagcgg gggccgggtc aggttgaatc cgaggtacac
     3721 ggcgttgcgc ccatcgtgcg ccgcgacctc gaaccctgcc gtccggccga ggcgatcgaa
     3781 gtgctccggc gcgaggtcgt tcaccacgag atcgccgctg ccgcgcagca tctcgagcag
     3841 gcgggcgctc tccgcaggga ggatccggat gcggacgctc gtgagggggc tcgccccttc
     3901 gaagtaggcg tcgaaccgcg tgaggagcag atcttcgtcc ttgcggtagc gctgcaggcg
     3961 gaacggaccg gtcccgatcg gggcgccccc ctcgcccgac gcgggcgcgt cgcgcaggat
     4021 cggaacggtg aggttcgaca ggaggggcgc gaacggccgg cgcaggcgga agacgaccgt
     4081 ccgggggtcc ggcgcctcca ccgactcgac cgactcgaag tcggcgcgcc ggaacgacac
     4141 cacctcgccg tcgaggatcg agcggtaggt gcgcgccacg tcccgggacg tgaggagcgc
     4201 gccgtcgtgg aaacggaccc cctcgcgcag gcgcaccacc agggtgcgtt cgtccgggga
     4261 cgcggccgac tccgccaggt cggggacgac gtcgccgtcg tcggcgatgc ggaacaggga
     4321 attgaagagg agatcgaaca ccctgcggct cgcttcgtcc gatcccagcc gcggatcgag
     4381 gctgttgggt ccgctgtcga cgatcacggt gatgccgtcc ggatcggcgg ggcgccggcc
     4441 gcacccggcg aaagcgagca ccccaagggc ggcgatcaaa agaccgctcc gcgcgatccg
     4501 cccctccata gggggcgcat catatttgat gcggcaccga tccggcgcct gcgtcggttg
     4561 tgagggcgtt ccgcttcccc tatacttccc ggccgcatga tgaagccctt gcatctcagg
     4621 cacttggagc cggccccggg aggccggcgc agggcccgcc gcgccgccgt cgccgtcatc
     4681 gtcctgctct tggcgggcgg aatcggcgcc gccgccgaga accggttcga gcgggtgatc
     4741 gtcctgggat tcgacggcgc cgacgcgcgc ctcgtgtcgc aatacatgga cgaagggagg
     4801 cttccgaacc tggcgcggct gcgggaccag gggacctacg cccccctccg cagcaccaat
     4861 ccgccgcaga cgccggtctc ctgggcgacc ttcgcgaccg ggctcaaccc tggccggacg
     4921 gaaatcttcg atttcctgcg gcgcgtcgag gggacctacc tgccggactt cgccatggtg
     4981 cgggaaggaa agcgcccctt cctgttgggc ccgaagaatc cgatggtcgt ggcgccgatc
     5041 gccttcgccg tcgtgggatt gctggcggcg gtcgggctgt ggctcgcggg tgcacgcggg
     5101 ctgcgcctcg cgctcggcgc cctggtcgcc ggggcgctcg ccgggggagg ggccgcgttc
     5161 ctcgcggcgc gctggctccc ggagtcggtt ccggacgcga cgaacgggcg gcgcggcctc
     5221 ccgttctgga ccgcggcggc cgcggccggg attccgagtc gcgtcatcca cgtccccgcc
     5281 aattttcccg ccgagagcca cgatcatctc gagcttttgt cgggactcgg cgttccggac
     5341 atgcgggggc ggatcggctc cccatcgctg ttcacctcgg acgcggcgct cgcggtcccg
     5401 gacaaccagt tcagcgtcga gatcgtgcat ctctccgggc ggcgcggccg catcgagacc
     5461 gccctcgcgg gtccgctgaa cgatccgttc tggaactatc ccatcgcgaa agcgaccgag
     5521 gggctcacgg gagacgagaa acaggcgaca cgcgcgcgcg tcgaggagac gctgaaggcg
     5581 cgcggggtgc gccggcggct cgacgtcccg ttcgtgatcg acgcgaccgg ctccgacctg
     5641 acgatcgatc tcggcggccg ccgcgagacg ctgaagcccc tggggtggag cgactggttc
     5701 gtcgtcccgg tggcggtcaa tccgctggtc gatcgagtcg cggggctgcg cggcatggtc
     5761 cggttcaagc tgctggcgct cgagccggag atccggctct acatgagccc gctgatgttc
     5821 catccgaagt gccagccggt gccgttcgcg acgccggccg gtttcgcggg cgagctcctc
     5881 gacgaggtcg gactgttcaa gacgctcggc tggccgatcg acacctggtc gctcccctcc
     5941 ggcatcgccg acgaacagca cttcctcgag gacctcgagt tcaccgtcgc gaccgaggtg
     6001 aagatgctgg atcaggcgct cgaacgcggc gacgcgcgcc tctacgtgca ggtgttcgac
     6061 ttcaccgacc ggatcgggca cctgttctgg cgctaccacg acccgggaca cccgatttac
     6121 gacgccgccg ccgcggggcg gtacgagccg gagatcccgc gcgcctacga gcggatggac
     6181 gccatcgtcg gccgcgcgat ggcgcgcctc gacggcaaga ccgcgctcct cgtctgctcc
     6241 gaccacggct tcgcctcgtt ccgccgcggc gtcaactaca acacctggct cgtgaggaac
     6301 ggcttcatga cgctcaaggg ggcgatgggc ggcggcggca agacgctcga ggatctcttc
     6361 gacaaagggg agatgggggg attcttcaag aacgtggact ggtcgcggac caaggcgtac
     6421 gccatggggc tcggcaacat ctacctgaac ctcctgggcc gcgagccgaa gggaagcatc
     6481 gccccgggcc gcgagtacga ggaggtgcgg gacgcgatca tcgccggact cgaggcgctc
     6541 gtcgatccga agaccggcga gaagccggtg acgaaggtgt acaagcggga ggagatctat
     6601 tccggcttcg acccgcgcgt cgtccccgac ctgcgcgtcg ccaacagcga gcactaccgc
     6661 atcggctggc agagtacgct cggcgaggtg ccgctggaga tcttcgagga caacaagaag
     6721 gcctggtcgg gggaccactg ctccaacgac ccggccatcg tgcccggcat cctgttctcg
     6781 agcgtccggc tgcagaagga gcgggagccg ttcatcggcg acctgttccc caccaccctt
     6841 gccctcctcg gtgttcccgc cgtcccggac atcgacggcc ggagcctgct gccctgatcg
     6901 ggatgatcgc gcggcggccc gtccggcccg cgaggcgaac gaccccgagc gcggcggctc
     6961 tcctcgtcgg cgccgccctc ctcggccccg ctctccatgg cgccgaaccg tcaccggccc
     7021 cggcggaaag cgggacgggg cggcccaagg atgattcgga cgcccggcgg ctcgcggcgc
     7081 tgcaggcgca gatcgaggcg ctgcgcgccc gcctcgtggc ggcggaggag caggccggga
     7141 cgatcctcga cgatctcgac gagatggggc tgcggctctc cctgctcgcg cgcgagtcgg
     7201 agatgctgca gcgggaaatc gacgccgcgc gcgtgcaggt gatgcgctcg cgacgcgagg
     7261 aagaggcggc acgggagcgg ctgacgaagg ccgagcagga cctgcgggac tggattctcg
     7321 agctgtacaa gtcggggcct cctccggatc tgagcctgat gctcctggcg gggagcccgg
     7381 cggaagtggc cggtgcgcag cgcagcgccg aggcgctggc ggtggcggag gggcgccgcg
     7441 tcgaggcggt ccgctccgag cgggagcggc tcgcggccgc gctcgcccag agggaacgcc
     7501 aggaggagcg gctgctcgat ctgcagaagg agttggagca gcgccgggcc gacctcggga
     7561 ccgcgaaagc gaagaagggg aggcttctcg acgacatccg cacccgccag gagagcgagg
     7621 aggaggcgct cgaggggatg gtgcagatgg agcgggacct gaccgccctc ctgggccggg
     7681 tccaggaccc ggcgagcccc tcgcgtgggc tggcccggtt ccacggcctc ctcgcctggc
     7741 cggccgccgg atcggtcgcc ctgcccttcg ggaacgtgcg gcatccccgc ttccacaccc
     7801 aggttcccca tccggggctg gaaatcgcct gcgaccccgg gacggaggtg cgggccttgt
     7861 tcgtggggcg cgtggtctat tcggattggc tccgcggata tggagaaatg attgtcatcg
     7921 accacggcga cgagtatctt tccatctacg gccagctcgg cgaacggctg gtggccgtgg
     7981 ggcaggaggt tcggcagaat cagccgatcg cccgctccgg gacggaagga acgttcggag
     8041 tcacaggact gtatctggag atccggcatc ggggggagcc cgaagacccg cttccgtggc
     8101 tccgccagag cggccgccgg cccgtctcca gggagaggaa aaaatgacgt tgtccctcga
     8161 tcgcaaagtg ctcgtggtgc tgtcggccct ggtggtcgtt ctgaccctcg ccgggggatt
     8221 cgccggccgg accggcgccg tcgaagggac gtacgactac ctcaaggtgt tcaacgaggt
     8281 gctgtacctg tcggtgaaca actacgtcga gccggcgcag atcgaagcgc tgatggacgg
     8341 cgcctaccgc ggcatgctcg agacgctcga tcccggcaac gagtacctca cccagcgcga
     8401 gtacgaccgg gcctcgcgcg gtgagtccgg cgggccggcc gaggtcggcc tgaggctctc
     8461 caagcgtcac ggttacatcg tcgtcatttc ttccctgccc gggagcccgg cggcggccgc
     8521 cgggctgaag accggcgatg cgatcctcac catcgacagc cgtccgaccc ggctcatggg
     8581 ggtctgggag gcggagcagg cgttgcgcgg gaagaccgga tcgaaggtga ccctcggcct
     8641 gagcccctcg gaagggggcg aacggcgcga gcaggcgctc gagcggcgca ccatcacccc
     8701 gcccgccccc tccggccgtc tcgcggagga gggggtcggc acggtgcgcc tcctcggtct
     8761 gcgcgacggc gacgcgaagc gcgtggatca ggcgatcgcc tcgctcaagg agcagggtat
     8821 gaagcgtctc ctcctcgacc tgcgcggatg cgtttcgact tcgctcgccg agggcattgg
     8881 tgtcgccagc ctgttcatga aggacggcgt cgtcgtgacc gtcgtggacc ggcacgatgg
     8941 cgacaaggcg taccgcgccg acgggcgcaa gcggtcctgg agtgggccga tggtcgtcct
     9001 cgtcgacgag gggacgagcc gcacctgcga ggtcgtgacc gcggccctgc gcgacggtct
     9061 cggaacgccg gtggtggggg agcgcacctg gggcgccggg acggtcatga gcctcctgcc
     9121 gctgccgaac ggcgatgggc tgttcctcgc gaccggcctg atgcagtccc ccgcgggcaa
     9181 ggaatggaac gccaaggggc tcgatcccga cctggcgatc ccgagcgaca ccgaggcggg
     9241 gaaggacacc cagcgccaga aggcgatcga ttacctgaaa ggggtcacgc gacagaccgc
     9301 gtccctctcg agctccgtgc ccggcacccc ggtcgcccgg aaccgggccg gcgccacagc
     9361 gcgccctgcg cgccccgaga acgacgggac atacatcgcc cgcgggctcc ccggcgctca
     9421 ccgcgaagcc gtctgacaac gcgcactcgg aaggcattgc tcccgccgct tcaaccctga
     9481 tcgagttgcc gtagtttccc cagcaccgcc tcccgtctca gcaccaagcc gctcgtgccc
     9541 cgccccggtg ccgatggggg cgcttttcgt tgcgtccgtc ggcacgaaac ctatattgga
     9601 gcctcggctc gaggcgcgga gcccgggggg gcggccgatc gtgagcatcc cgaagaacat
     9661 cggccgttac gaaatcgtcg aagaggtcgg ccgcggctcg atgggcgtcg tctaccgcgc
     9721 ccgcgacccg cgcctcggcc gcattgtcgc cctcaagtgc gtcgccttct ccttcccgct
     9781 cggtccgggg gaggaggagg agttcctgcg ccgcttctac ctggaggcgc agatcgccgg
     9841 ccgcctgagc caccccaaca tcgtcaccat ccacgacgtc ggcacccgcg agaccgaggg
     9901 gaccgcgttc atcgccatgg agtacgtcac cgggacgaac cttcaggagc tcctggcggg
     9961 gggtggacgc ctgcccatac ctcaagttgc agacgtggcg cgccagatgg cggacgcact
    10021 cgactacgcg cacggctgcg gggtcgtgca ccgggacatc aagccgggga acatcgtcct
    10081 caccaccggc ggccaggtga aggtgctcga cttcgggatc gcgcgcgccc cctccggcga
    10141 cagcacgaaa ccggggcgga tgctcgggac tccgaactac atggcccccg agcaggtcac
    10201 cggcgcgacg gtggacggac gcgccgacca gttctccctc tcggtgacgc tctaccatct
    10261 cctgaccggc gagcgcccct tcgtcggcga aagcctgacc gccatctcgt accaggtggt
    10321 gaacgtcacg ccgcccccac cgtcgcgcct caatccggcg ctgcccgccg aagtcgatcg
    10381 catcctggcg cgcggactgt cgaagaacgc cccggaccgc taccccggct gcggtgatct
    10441 cgcctcggag ttcggcgccg ccctggccac atggaaggag gcggtcgatc gcgccgttcc
    10501 ccggactctg gtggcggcga ccgacgcgtc gctgcgtgcc ggcgtctcga ccgggccgtc
    10561 ggcggggcga ggcctggccg gggtcctctc cggagccggc cccggagcgg tggccggctg
    10621 gacgctcttt ctcggggccc tcggagcgct cgccctctct ccctatctcc tcaacgcggt
    10681 gacccgcgag gatcgcaccg gcggtccggc gcagcgcccg cgcccgggcg cctcgcaggt
    10741 cgcgccgccg tcgtggcttc ggctgccgcc ggcgccgagc cccgcccctc cacgcgcggc
    10801 gaagccggca ccggtcgcgc gcggcaccct cctcctcacc ttccagcacc gtttcgcgag
    10861 cgggatggtg acggtgcggg tcgacggcgc cgaggcggtg cgcgagcgaa tcagcgccag
    10921 cggccggacg acctggacga aatcgattcc gctcgccgcg ggccggcgcc gcgtcgaggc
    10981 gcgcatcgaa gcgaaccggg gagacgtcga cgaaatcgcc gggatcgatc tcgagatccg
    11041 ggagcaccgg acgcggacga tcgtgctctc catgaatgcg ctgacgcgcc ggctgaagat
    11101 ccgtcccgag gaggaccaac cgcgatgagg gctcgtcgcc gaagcgcact cctcgcctcc
    11161 tgtctcgcgc tcctcgcggc aggtgccgcc gtcgccgacg accgcccgct catcggcccg
    11221 cgggcccgca cgtacgcggt gttcctcgaa gcgcagaacc tcctcctggc gggccgggcc
    11281 gacgaggccg cgcggcttct cgacgccgcc gtctccgccg accccgaccc ggggctcctc
    11341 ctggaagcgg cgcgcatgca cgagctcctc ggcgagccgg accaggcgct gccgctcgcc
    11401 gaccgggcgc tcctggcgcg tcccggctgg ccggaggcgc tggtgctgcg cgccgggctc
    11461 cgactcgatc gcgacgatga gccggcgccc gagcatcgcc tggcctctct cgccgatctc
    11521 cgcgtggcgc tcgccaccga tccgtcgtcc gaagaggggg cgcgccggct ggccgcgatc
    11581 tgcgcaggat ccgactgcgc cggagaggcg gcgcgggtgc tcgaggcgca gcgggcgaac
    11641 cgcgtgctgc cggtcgatct cgcgctggcg ctcgcgcggc tcgatctcga gatcgggaag
    11701 gtggaggagt gccgggcgat cctgaccgag attctgcagc gcgatc
//
LOCUS       DASRIQ010000607         5900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3116640, whole genome shotgun sequence.
ACCESSION   DASRIQ010000607 DASRIQ010000000
VERSION     DASRIQ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3116640"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VFD06_15035"
     CDS             <1..1163
                     /locus_tag="VFD06_15035"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZN04898.1"
                     /translation="MEPVNATAHVEKDRCTIWAPTQAPGWAATEVMKLTGLPKEAIRL
                     NITYLGGGFGRRLTPDFVLDAVEISRAAKAPIKMTWTRPEDLQHDHYRPFSHHRLAAV
                     LGEGGLPAAWRHRIVSTSISESMEPGNAHPESDELACASDLPYAIPNLRVEYAPAPSP
                     VPRGWWRSVDASFNVFVVESFLDECAAAAKQDPLRYRLALLKEPRRIPYPGSDLVLET
                     ERLKKVLELAAEKAGWGTSPPAGRARGIAAAFSYRSYFAQVAEVEVTSKKRLKVHRLV
                     AAIDCGRVIHPGILASQIEGGAVFGLAAALKHAITIEGGRVTQSNFDDYDMLRIDEMP
                     RVEAHAVPSDGPPTGVGEPGVSCAAPAVFNALFAATGKRIRRLPLLPGDLGA"
     gene            1223..1582
                     /locus_tag="VFD06_15040"
     CDS             1223..1582
                     /locus_tag="VFD06_15040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZN04899.1"
                     /translation="MNRRQKQGEVRYRRRLEVRFSDGANPSSIGFSGNLSLQGMMIRT
                     PRVYAPGTILDLELRFAGGTVKVRGRVMWAREGPMVWISSGRIGMGIRFIDPPENLAE
                     MIASGPAVVDPARSAAL"
     gene            complement(1590..3911)
                     /locus_tag="VFD06_15045"
     CDS             complement(1590..3911)
                     /locus_tag="VFD06_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN04900.1"
                     /translation="MTLNRKAARTLLALIGLALLTPGVSRPEPKKAYVDSMLRRMIRT
                     SEKAVREHGRPDAVPADEMAFFTRVVAAEIREGRPSVRVRLQLDDTARRALGDIGIKT
                     YGKMAGFASAIVPLDRLTEVAAIDGVQRMQAVRVPKLELNVSKPETGSSAMATTYGAS
                     GAGVIYGAIDSGLDWNHQDFRNANGTTRIKFLWNQDDACVGVAPPPPFDFGCLYTEAE
                     INAALTGGPTITAPDGDGHGSHTTGVGAGNGRGTGNGQPANTYVGMASQADIVFVKIF
                     PEPGDANGCATCFAISDAMDFIDAKAAEMDKPFVINMSFGSQYGAHDGSDLDEVTIDT
                     LTGPGIPGKATVKSAGNERGNRIHFSGTLAQGQTITHQLVVPTYTPLPGAFNDTVAWS
                     IWYSNGDILTVRINDPTTTPCGSTVLTISNTTGGGNVGTNTTSGTMIIDDSTSPLPNG
                     TRFFDAEIDDQATAAPCRGTWQFQVTGTNITAGGRYDGWIWYNGFGGTQAPALWAAAS
                     ADLTNLLSIPGSAIHTTTVGAYATKPSWLAFDGNTYQWNPPPGNGSLASFSSPGPTRD
                     GRLKPEITAPGFGVSSTLSIDATPLAPADQPLLMPDTVHWILPGTSFSAPHVAGIYAQ
                     LLSMNPTLDALQLRQILTATARVDAFVTPVPNNDWGFGKVGALQAANVVDALNQMAPA
                     IFMTQSGRFSFVGSPGADTYNVYRGLLSARSLTNYGACFLSDLPANSFNDASAPPVGD
                     GYMYYMTAVTGGVEGSLGNRSNGTARPNLAPCP"
     gene            complement(4036..4893)
                     /locus_tag="VFD06_15050"
     CDS             complement(4036..4893)
                     /locus_tag="VFD06_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZN04901.1"
                     /translation="MIRLVEMGPRDGLQNEPRHVPVDVKVAFIDALSESGLEEIEVSA
                     FVSARAIPQLADAGEVFAAIRRRPGVVYSALVPNERGLERALLARADKVAVFTAASET
                     FNRHNIHAGIQESFDRFKPVIEGARRARLPVRGYVSTAFWCPYEGKIAPETVVEVIRR
                     LDALGVDEVSVGDTIGKAVPEEVNRLLDLLLAHLPAPKIAMHFHDTYGHAVENVLAAF
                     ARGITIFDASTGGIGGCPYAPGASGNVSMERVIAALKGAGVEVTVDSGALAKARDVIE
                     PSLGRELGT"
     gene            complement(4890..5219)
                     /locus_tag="VFD06_15055"
     CDS             complement(4890..5219)
                     /locus_tag="VFD06_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HZN04902.1"
                     /translation="MSRRLTVVRTPSGVWVGWPGGAKFFAPERPGSAAVDANGASEVR
                     APMTARVVKVSARPGGEVGADEVLVILQAMKMEYRLTSPRAGRVERVHCAEGEMVDLG
                     ATLVTLA"
     gene            complement(5216..>5900)
                     /locus_tag="VFD06_15060"
     CDS             complement(5216..>5900)
                     /locus_tag="VFD06_15060"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methylcrotonyl-CoA carboxylase"
                     /protein_id="HZN04903.1"
                     /translation="PDGAHYFLEVNTRLQVEHPVTEMLLGVDIVAAQIGLAQGGALPR
                     PAEPRGHAIEARLNAEDAHRGFLPSSGRVLVADWPNRPGVRVDTGIRAWSDVRPEYDP
                     LLAKVIAWGADREQARRRLVEGLRDTTILGVATNLSFLLQVLESAPFRSGATHTTLIE
                     STRWPEPEIPDEALAAARRALQSPAGRGTEGDGGEAPAVASDASAAASDADRFSPWRS
                     DGDASRFRP"
BASE COUNT          859 a         2062 c         2079 g          900 t
ORIGIN      
        1 ccatggagcc ggtgaacgcc acggcgcacg tcgagaagga ccgctgcacc atctgggccc
       61 ccactcaggc tcccggctgg gcggcgacgg aggtgatgaa gctcaccggc ctgccgaagg
      121 aggcgatccg cctcaacatc acctatctcg gaggcggttt cggccgccgc ctcacgcccg
      181 acttcgtcct cgacgccgtc gagatctccc gggcggcgaa ggcgccgatc aagatgactt
      241 ggacgaggcc ggaggatctg cagcacgacc actaccggcc gttctcgcac catcggctcg
      301 ccgccgtgct gggggagggg gggctgccgg ccgcctggcg gcaccgcatc gtctccacgt
      361 cgatcagcga atcgatggag ccggggaacg cgcacccgga gtcggacgag ctggcctgcg
      421 ccagcgatct cccctacgct atcccgaacc tgcgcgtcga atacgccccg gcgccgagcc
      481 cggtgccgcg cggctggtgg cgatcggtgg acgcgtcgtt caacgtcttc gtcgtcgagt
      541 cgttcctcga cgagtgcgcc gcggcggcaa agcaggaccc gctgcgctac cggctggcgc
      601 tcctcaagga gccgcgccgc atcccgtacc cggggtcgga cctggtgctc gagacggaac
      661 gcctgaaaaa ggtgctcgag ctcgcggcgg aaaaagccgg ctgggggacc tccccgccgg
      721 ccggccgggc gcgcggcatc gccgccgcct tctcctaccg gtcctacttc gcgcaggtgg
      781 cggaggtcga ggtcacgtcc aagaagaggc tcaaggtgca ccggctggtg gcggcgatcg
      841 actgcggacg cgtgatccac ccggggatcc tggcatcgca gatcgaaggg ggggcggtgt
      901 tcggcctcgc ggccgcgctg aagcacgcga tcaccatcga gggggggcgc gtgacgcaga
      961 gcaacttcga cgactacgac atgctgcgca tcgacgagat gccccgcgtc gaggcgcacg
     1021 cggtcccgtc cgacgggccg ccgaccggcg tcggggagcc gggggtctcg tgcgccgccc
     1081 cggcggtctt caacgccctg ttcgccgcca ccggcaagag aatccgccgc ctgccgctcc
     1141 tcccgggcga cctcggagcc tgagcggctt gacggagggc atgcccggga cgcacctttg
     1201 agggcatgcg gagcgtctcg aggtgaatcg ccggcagaag cagggggaag tccggtaccg
     1261 gcgccggctc gaggtgcgct tcagcgacgg ggcgaacccc tccagcatcg ggttctccgg
     1321 gaacctctcg ttgcagggga tgatgatccg gacgccgcgc gtctacgcgc ccggcaccat
     1381 cctcgatctc gagctgcgct tcgccggcgg gaccgtcaag gtgcgcggac gggtgatgtg
     1441 ggcccgcgag gggccgatgg tctggatctc cagcggccgc atcggcatgg ggatccggtt
     1501 catcgacccg ccggagaacc tggcggagat gatcgcctcc gggccggccg tcgtcgaccc
     1561 ggcccgcagc gccgccctct agaccccggt tacgggcagg gggcgaggtt cggtcgcgcc
     1621 gtcccgttcg accggttccc caggctccct tcgaccccac cggtcacggc cgtcatgtaa
     1681 tacatgtagc catcgccgac gggcggcgcc gacgcgtcgt tgaaggaatt cgccggcagg
     1741 tcgctcagga agcaggcgcc gtagttcgtg aggctcctcg ccgacagcag cccgcggtag
     1801 acgttgtagg tatccgcccc cggcgacccg acgaaggaga accgccccga ctgcgtcatg
     1861 aagatcgccg gcgccatctg gttcagcgcg tcgaccacgt tcgccgcctg cagcgccccg
     1921 accttgccga acccccagtc attgttcggc accggcgtga cgaaggcgtc gacccgcgcg
     1981 gtcgcggtga ggatctgccg cagctgcagg gcatcgagcg tcgggttcat cgacaggagc
     2041 tgcgcgtaga tgccggcgac gtgcggcgcc gagaaggacg tccccggcag gatccagtgc
     2101 accgtgtcgg gcatcaggag cggctgatcg gccggggcga gcggggtcgc gtcgatcgac
     2161 agcgtcgacg agactccgaa gccgggggcg gtgatctccg gcttcagccg gccgtcgcgg
     2221 gtcggccccg ggctcgaaaa ggaggccagc gagccgttgc caggcggcgg gttccactgg
     2281 taggtgtttc cgtcgaacgc cagccaggag ggcttggtgg cgtaggcacc gaccgtcgtc
     2341 gtgtggatcg ccgagccggg gatggaaagg agattggtga gatcggcgga ggcagccgcc
     2401 cagagcgccg gggcctgggt ccccccgaag ccgttgtacc agatccagcc gtcgtagcgg
     2461 ccgcccgcgg tgatgttcgt gccggtcacc tgaaattgcc aggtgccgcg gcacggcgcg
     2521 gcggtggcct gatcgtcgat ctccgcgtcg aagaagcggg tgccgttcgg cagcggcgac
     2581 gtgctgtcgt cgatgatcat cgtgccgctg gtcgtgttcg tgccgacgtt cccgcccccg
     2641 gtggtgttcg agatcgtcag gacggtcgag ccgcacgggg tcgtcgtcgg gtcgttgatc
     2701 cggaccgtga ggatgtcgcc gttgctgtac cagatgctcc acgccaccgt gtcgttgaac
     2761 gcgccgggga gcggcgtgta ggtcggcacc accagctgat gggtgatcgt ctgaccctgg
     2821 gccagcgtcc cggagaagtg aatccggttg ccgcgctcgt tgcccgccga cttcaccgtc
     2881 gccttgcccg ggatccccgg gccggtcagc gtgtcgatgg tcacctcgtc gaggtccgac
     2941 ccgtcgtgcg ctccgtactg cgacccgaac gacatgttga tgacgaacgg cttgtccatc
     3001 tcggccgcct tggcgtcgat gaagtccatg gcgtcggaga tcgcgaagca ggtggcgcag
     3061 ccgttcgcgt cacccggctc ggggaagatc ttcacgaaga cgatgtcggc ctgggacgcc
     3121 atcccgacgt aggtgttggc cggctggccg ttgccggtcc cgcgcccgtt gccggcgccg
     3181 acgccggtgg tgtgcgagcc gtgtccgtcg ccgtccggcg cggtgatcgt cgggccaccg
     3241 gtgagcgccg cgttgatctc cgcctcggtg tacaggcagc cgaagtcgaa cgggggcggc
     3301 ggcgcgacgc cgacgcacgc gtcgtcctgg ttccacagga acttgatgcg cgtcgtgccg
     3361 ttcgcgttgc ggaagtcctg gtggttccag tcgaggccgc tgtcgatggc gccgtagatg
     3421 acccctgcgc cggaggcgcc gtaggtggtc gccatcgcgg acgaaccggt ctcgggcttg
     3481 ctgacgttga gctccagctt cgggacgcgc accgcctgca tgcgctgcac cccgtcgatc
     3541 gccgcgacct ccgtgagccg gtcgagcggc acgatcgccg acgcgaagcc ggccatcttc
     3601 ccgtaggtct tgatcccgat gtctccgagc gcccggcgcg ccgtgtcgtc gagttgcagc
     3661 cggacgcgca cgctcggccg gccctcgcgg atctccgccg ccacgaccct cgtgaagaag
     3721 gccatctcgt cggcggggac ggcgtcggga cgcccatgct cgcgcaccgc cttctcggag
     3781 gtgcgaatca tgcggcgcag catggagtcg acgtacgcct tcttcggctc cggccgcgag
     3841 actccggggg tcaggagagc cagtccgatc agggcgagca gggtgcgcgc cgctttacga
     3901 ttcagggtca tcatcaacct catcagcgtc acgacctcat taaatggaga ttcggctcag
     3961 gcgggcgttc cccgcgcagg aacgccgcac cggtcttggc ttggaaccta tttagtacga
     4021 ccattttggg ccaaatcaag taccaagttc gcgccccagc gacggttcga tgacatctct
     4081 cgccttcgcg agcgccccgg aatcgaccgt gacctccacc ccggccccct tcagagccgc
     4141 gatcacgcgc tccatcgaga catttccgct cgcccccggg gcataggggc acccgccaat
     4201 ccccccggtg ctggcgtcga aaatcgtaat tccccgggcg aatgccgcga ggacattctc
     4261 gaccgcatgt ccataggtgt catggaaatg catggcaatc ttcggcgcag ggaggtgcgc
     4321 cagaaggagg tcgagcagcc ggttgacctc ctccgggacc gccttgccga tggtgtcgcc
     4381 gaccgagact tcgtccaccc ccagggcatc gagccggcgg atcacctcga ccaccgtttc
     4441 gggggcgatc ttcccttcgt acggacacca gaaggcggtt gagacgtacc cgcgcaccgg
     4501 gaggcgggcg cgccgcgccc cctcgatcac cggcttgaac cggtcgaacg actcctggat
     4561 cccggcgtgg atgttgtgac gattgaaggt ctcgctcgcc gccgtgaaga ccgccacctt
     4621 gtccgcgcgg gccagaagcg cccgctcgag cccgcgctcg ttcggcacca gcgccgagta
     4681 gacgaccccg ggccgacgcc ttatcgccgc gaagacctcc ccggcgtcgg cgagctgcgg
     4741 gatggcgcgc gccgacacga atgcgctgac ctcgatctct tcgaggccgc tctccgagag
     4801 cgcgtcgatg aaggcgacct tgacgtcgac cgggacgtgg cgcggctcgt tctgcagccc
     4861 gtcgcgcggg cccatctcga cgaggcggat catgccaggg tcaccagcgt cgcgccgaga
     4921 tcgaccatct ccccctcggc gcagtggacg cgctccaccc gcccggcgcg gggtgaggtg
     4981 agccggtact ccatcttcat cgcctgcagg atgacgagca cctcgtccgc ccccacctcg
     5041 ccgccgggcc gcgccgaaac cttcacaacg cgcgccgtca tgggcgcgcg gacctccgaa
     5101 gcgccgttcg cgtcgacggc cgcgctcccg ggacgctccg gcgcgaagaa cttcgcgccc
     5161 cccggccagc cgacccagac ccccgacggc gtgcgcacca cggtgagccg ccggctcacg
     5221 ggcggaacct cgaggcgtcg ccgtcggagc gccagggaga gaagcgatcg gcgtccgacg
     5281 ccgcggccga ggcgtccgac gcgacggccg gtgcttcgcc gccgtcgccc tccgttccgc
     5341 ggccggccgg agattgcagg gcgcggcgcg ccgccgccag cgcctcgtcc ggaatctccg
     5401 gctcgggcca gcgggtggac tcgatgagcg tcgtgtgggt cgccccgctc cggaacggcg
     5461 cgctctcgag cacctggagg aggaacgaca ggttggtcgc gacgccgagg atcgtggtgt
     5521 cgcgcagtcc ttcgacgagg cggcggcgcg cctgctcccg atcggccccc caggcgatca
     5581 ccttggcgag gagcgggtcg tattccggcc ggacgtcgct ccaggcgcgg atgccggtgt
     5641 ccacgcgcac gccggggcgg ttcggccagt cggcgacgag cacgcgcccg ctcgagggca
     5701 ggaagccgcg gtgcgcgtcc tcggcgttca gacgcgcttc gatggcgtgg ccgcgcggct
     5761 cggccggtcg cggcagcgcc cctccctgcg ccagcccgat ctgcgctgcg acgatgtcga
     5821 cgccgaggag catctcggtg accggatgtt cgacctgaag ccgggtgttc acctcgagga
     5881 agtagtgggc gccgtccggg
//
LOCUS       DASRIQ010000608         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2579057, whole genome shotgun sequence.
ACCESSION   DASRIQ010000608 DASRIQ010000000
VERSION     DASRIQ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2579057"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFD06_15065"
     CDS             complement(<1..200)
                     /locus_tag="VFD06_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04904.1"
                     /translation="MGTMMSDSKAGPVSLLLALALVSPHLLLSESAGAEGPVVVGRVR
                     VTPVETPSQERARAIERLLERR"
     gene            392..>2093
                     /locus_tag="VFD06_15070"
     CDS             392..>2093
                     /locus_tag="VFD06_15070"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HZN04905.1"
                     /translation="MTGLLAMALLGLLLDGGVRAQGPVAARAETIRLVPGHPESRGKG
                     RIALVPDASALRRLFRAGSARVADVPLPDGRRIDLDVASTDVTTGRTRFFVGGPEGLR
                     ETAPPDMRFFRGRVAGDPESLVSLSLFDRRIAGFIRVQGREYTMSPRAFSLDSPEASE
                     IEMVDEALEGGPSGVCDGEEAADIAGVMPLPDGPGLESSTASSIDANTLLAGTIAVEG
                     TVEWVARHGGVAAATAYTLNLMAQVSAVYESDVKVRLEVPYLLMNAAEPDGYSGGSNS
                     TSTVLSEMRAKWNGTTSLRGVFRTAAHVFSTYSSGGAGRAYINVLCSNVPVNPSAYDY
                     GVTLLEGKGGSWERRLVAHEIGHNFSSPHAHCYTPEIDKCYNAETGCYAGPVVQTTGT
                     VMSYCNVRLSTFHQRERDERIRPGAQAAYPRCMEVAGTPGDTGGLRVMAAGRCATGNL
                     QSDDGATNGSYGYSGTSQGAWIKRFKPACYPFKLTDIDVRMTNTTVAPGRAIRILVYR
                     DPAGSGTPVGASLAYTEDTAVQVVGAGTWNRFTLEDPVIVTSGDLYIGFYDLVADAGT
                     T"
BASE COUNT          352 a          714 c          715 g          312 t
ORIGIN      
        1 aagcggcgct cgagcagccg ttcgatcgcc cgggcgcgct cctgggatgg tgtctcgacc
       61 ggcgtgaccc gaacccgccc gacgacgaca gggccttccg cccccgcgga ctcagacagg
      121 agaaggtgcg gggatacgag agcaagagcc aggagcaacg atacgggacc ggccttgctg
      181 tcgctcatca tcgtccccat gaaggatcct cgcacgttac tcccgcggcc cgacaacttc
      241 aaaggaaaag acagttgaca ttcccggata cgatcgttga cgatagaaac cccatcacac
      301 tctcgtcacc cctcccgaca agctgtgtga cctggaggtc atggctcgtc tcccgatcga
      361 cacagggtcg cgctgtcgta ttgccttccg cgtgactgga ctgctcgcga tggcgctgct
      421 cgggcttctt ctcgatggtg gcgtcagggc gcaggggccg gtcgcggcgc gcgcggagac
      481 gatccggctc gtaccggggc accctgagag caggggcaag ggacggatcg cgctcgtccc
      541 cgatgccagt gcgctgcggc gcctcttccg cgccggcagc gcgcgggtcg ccgatgtccc
      601 gcttccggac gggcgccgca tcgatctcga cgtggcatcg accgacgtca cgaccgggcg
      661 gacgcgcttc ttcgtgggcg ggccggaagg cctccgcgag accgcgccac cggacatgcg
      721 attcttccga gggcgggtcg ccggggaccc ggagagcctg gtctcgctga gcctcttcga
      781 ccggcgcatc gccgggttca tcagggtcca gggccgcgaa tacaccatga gcccgcgcgc
      841 cttctcgctc gacagccctg aggcgtccga gatcgagatg gtcgatgaag cgctcgaggg
      901 cgggccttcg ggtgtgtgcg acggggagga ggcggccgac atcgccggcg tcatgccgct
      961 cccggacgga cccggtctcg agtcgtcgac cgccagcagc atcgatgcga acacgctcct
     1021 tgcggggacg atcgccgtcg aggggacggt cgagtgggtc gcgaggcacg gcggggtcgc
     1081 ggccgcaacg gcctatacgc tcaatctcat ggcccaggtg tcggccgttt acgagagcga
     1141 cgtcaaggtc cggctcgaag tcccctacct cctgatgaac gccgccgagc cggacggcta
     1201 cagcggcgga tcgaacagca cctcgaccgt gctcagcgag atgcgcgcca aatggaacgg
     1261 cacgacgtct ttgcggggcg tcttccgcac cgccgcgcat gtcttcagca cctattcgtc
     1321 gggcggcgcg ggccgtgcgt acatcaatgt gctgtgcagc aacgtgccgg tgaaccccag
     1381 cgcctacgac tacggcgtca ccctgctcga gggaaagggc ggctcgtggg agcggcggct
     1441 ggtggcgcac gagatcgggc acaacttctc gagcccgcat gcccactgct acacgcccga
     1501 gatcgacaag tgctacaacg ccgaaacggg ctgctatgcc ggcccggtgg tgcagaccac
     1561 cggcacggtg atgtcgtact gcaacgtccg gctgagcacc ttccaccagc gcgagcgtga
     1621 cgagcggatc cggccgggcg cgcaggcggc ctacccgcga tgcatggagg tcgccggcac
     1681 gcctggcgac acgggcgggc tccgggtgat ggccgcgggc cgctgcgcga ccgggaatct
     1741 gcagagcgac gacggcgcca ccaacggcag ttacggctac tccggaacgt cgcagggggc
     1801 ctggatcaag cgcttcaagc cagcgtgcta cccgttcaag ctcacggaca tcgacgtcag
     1861 gatgacgaac accacggtcg ccccgggccg ggccatccgc atcctggtgt acagggaccc
     1921 cgccggatcg gggactccgg tcggcgcgag cctcgcgtac accgaggaca ccgcggtcca
     1981 ggtggtcggc gccgggacct ggaaccggtt cactctcgaa gacccggtca tcgtgacttc
     2041 gggagacctc tacatcggct tctacgacct cgtcgccgac gcggggacca cct
//
LOCUS       DASRIQ010000609         6225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3646342, whole genome shotgun sequence.
ACCESSION   DASRIQ010000609 DASRIQ010000000
VERSION     DASRIQ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3646342"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="VFD06_15075"
     CDS             <1..1883
                     /locus_tag="VFD06_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04906.1"
                     /translation="GLVTPETGPGAGSAAIAAGRAGAVRPSKAREVAITIGASIVAAF
                     AGVVATRLIDRTTPPVSATHLTSVARLTHDVGHSEWPTWSSDGASIAFASDRDGDFDI
                     YVRRVEGGQEVNVTDDPGQDYQPAYSPDGETIAFISTRSSRSGMIKIGATFGLEFRTY
                     GGDLWTVPSLGGQAHRVAEDANAPAWHPDGRRIAYVSGREEQRSILEVAPDGGEPKPL
                     LRGEESKWEIVKLRYVPGGRWLSFDTPQSIVWILPAEGGTPRQLFEGASHVWDGSGKR
                     VYYLVRNQEGGALLRSVEFDAATGSVLGAASTIGVLTGILRDLAISADGRRLAVAELE
                     GSLNLTLLPLTADGARPSGPEEILSTGQVIDRYPAFSPDSQRIAYTSDRLGPMEIWVH
                     DLKTGRQSRLRLPGRDVGANLPLWLADGRSLVLLRSFGTGVYGIWKVAVDGSIAKELV
                     PPTQDLGTGIAISVDGRRLAVTVRRDGYLQIDVIDLETMERRPLTTSPSNKFAEDWSP
                     DGRSITFGSNAGGTMQIWRVPAAGGKEEPLTSEPERMRHASYSPDGRTIYVQPSHRNI
                     VRIPADGGAMTPVTTFPESSLFIEEPVVSPDGRALAYCRSHGGSSLWFLTIGTGSKGG
                     AK"
     gene            complement(1888..2427)
                     /locus_tag="VFD06_15080"
     CDS             complement(1888..2427)
                     /locus_tag="VFD06_15080"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04907.1"
                     /translation="MTHVRLTVVPALLIGAVVGAAAALGGSIVFAAKKPAAPLDFSGD
                     KKAVAERLLVEAERLAGSGSWERIGVGRVRYLMGDKAGGERLFNSVAKPVPSDLRRIA
                     ATYAAAGEWAKAKEYYERAHKEKPGDGGIAVEAGAWCNLKGDRARAEELFTKAFTEDR
                     EDFWNHVIAAGSYVGVEPF"
     gene            complement(2455..3747)
                     /locus_tag="VFD06_15085"
     CDS             complement(2455..3747)
                     /locus_tag="VFD06_15085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN04908.1"
                     /translation="MTTTRSKFPPVFWVANAIEVLERFAYYGIYFGFGIYMTHLGYTR
                     DQLGIVQSLFLCVSYGIPVISGSFADRFGFKKVLIVSYLAYLPSILLLLATRSLSGIA
                     LTMLSIGLAAGIFKPLIAGTVRVTTDSTNKTLGFGIFYAMVNIGGSFGPIVAGYLRAI
                     SWNYAFAAAAVSIVIMLIITIFFYKEPPRETRGKSVGETLREIGVTLSDLKFSTFLIL
                     LGFFFWLPFWAFFNLCALYIDSNLDTARLYQDLSSVLGSGIAGFLSHEGEDGVRRILG
                     ETIAHTGWVIMVFQVFVSKAAERFRAMPAFMLGLFIAGIGFFVIGRAAGGPPSIVLLG
                     ITLFAIGEMICSPRIQEYITWIAPKDKAGLYMGSNFLATMIGGLTSGFIYTSLSNHFV
                     QSGAPGRVWDVMGLHMIAAIAVFWLFGRIAGEFKELEA"
     gene            complement(3764..5557)
                     /locus_tag="VFD06_15090"
     CDS             complement(3764..5557)
                     /locus_tag="VFD06_15090"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HZN04909.1"
                     /translation="MKLASIAALWQDTKSTFLRFPAVIVSGVLAAAVSIHHFNDRDGG
                     EGPMRHVMVLALAIPLFFALRLFQEKPPWGRPLSRAAVWGGVVAGVALLLLYRRWLDH
                     ETGDAGFLRYAQLTLAVDLAVAIAPFLGRGETNGFWQFNRRLFLRFFFSGLYAAVFFG
                     GLALAILAVDHLFDVKVNPSLYPRLWFFSVFVFLPWHFLAGVPRDLTALDLDREHPKG
                     LRIFSQYMLVPLVTLYVLILYAYMAKILVSRQWPQGWVGWLVSFASVFGVLTILLLQP
                     GRGEREHRWIDRFARGFDAAILPLLGLLFAALGKRVGQYGVTERRYWLFILGAWLVGV
                     ALFMLFRRAPSIKVIPATLAVLALATSFGPWGTFEVSLRSQGRRLEALLARHGLLVAG
                     KLRPSADRVPPDDVKEISAIVDYLASSHGPAPLDRLMREPAPPGHAKPWKDGQDIRDA
                     FMNEMKLAYLRPWEQEASPDTNSYHTQDTARDVAGYELLYDCYFMRDTPTGATSRHWC
                     RLDEHGRAVEVLTGEVVLLRIPVAPLLERLASLAPQGGGVVPAADLCLMGGDERLRAK
                     ACFLTLNTRNDDAGARAAWTGNGYLLIDEHP"
     gene            5670..>6225
                     /locus_tag="VFD06_15095"
     CDS             5670..>6225
                     /locus_tag="VFD06_15095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZN04910.1"
                     /translation="MGGFFDTWIIPIVSVLFIVDPLAAVPAFLTMTDGDPDEKRRATA
                     LKAAIVSTGILLLFATAGSVVLRIFGVTLPAFRIAGGVILGLSALEMLRAERPSRETP
                     GEIREGIEKPDISITPLSIPLLAGPGAISTVMVLAPAHHTWRASIPVYAAILLTGLVT
                     WALLNRSALLQRILGTTGIHVMSRL"
BASE COUNT         1049 a         2154 c         2111 g          911 t
ORIGIN      
        1 aggggctcgt cacgccggaa acggggcccg gcgccgggag cgccgcgatc gcggcgggcc
       61 gggccggcgc ggtgcgcccc tcgaaagcca gggaggtggc gatcacgatc ggggcgtcga
      121 tcgtcgccgc gttcgccggc gtcgtcgcga cgcgcctcat cgaccgcacg acacctccgg
      181 tgagcgcgac ccatctgacg tcggtcgccc ggctcaccca cgacgtcggc cactcggagt
      241 ggccgacctg gtcctcggac ggcgcgtcga tcgccttcgc atccgatcgc gacggcgact
      301 tcgacatcta cgtccgccgc gtggaagggg gacaggaggt caacgtcacc gacgacccgg
      361 ggcaggacta tcagcccgcc tactcccccg acggggagac catcgccttc atctcgacgc
      421 ggtcgtcgcg ctccgggatg atcaagatcg gcgcgacgtt cggcctcgag ttccgcacct
      481 acggcgggga tctctggacg gtcccgtcgc tcgggggaca ggcgcaccgc gttgcggagg
      541 acgccaacgc gccggcctgg catccggacg gccggcgcat cgcctacgtc agcggccgcg
      601 aggagcagcg ctcgatcctg gaggtcgccc ccgacggggg agagccgaag ccgctgctgc
      661 ggggcgagga atcgaaatgg gagatcgtca agctgcgcta cgtgccggga ggccggtggc
      721 tctccttcga cacgccgcag tccatcgtgt ggattctccc ggcggagggc gggactccgc
      781 ggcagctgtt cgaaggggcc agccacgtct gggatggttc ggggaagcgc gtctattacc
      841 tggtgcgcaa ccaggaagga ggagccctgc tgcgcagcgt ggagttcgac gccgcgaccg
      901 gctcggtcct cggggccgcg tccacgatcg gtgtgctgac cgggatcctg cgcgatctcg
      961 ccatatcggc cgatggccgc cgtctcgccg tcgccgagct cgaaggctcg ctcaacctca
     1021 cccttctgcc gctgaccgcc gacggcgcca ggccttccgg accggaggag atcctcagca
     1081 ccgggcaggt catcgatcgc tatcccgcct tctcccccga ctcacaacgc atcgcctaca
     1141 ccagcgaccg gctcggcccg atggagatct gggtgcacga tctgaaaacg ggccggcaga
     1201 gccggctgcg cctccccggg cgggatgtcg gcgccaacct gccgctctgg ctcgccgacg
     1261 gccggagtct cgtcctgctg cgttccttcg ggaccggtgt ttacgggatc tggaaggtcg
     1321 ccgtggacgg cagcatcgcc aaggagctgg tgccgccgac gcaggatctc ggcaccggca
     1381 tcgccatctc ggtcgacggg cgccgcctcg cggtcacggt gcgcagggac ggatacctcc
     1441 agatcgacgt catcgatctc gagacgatgg agcgacgtcc tctcacgacg tccccctcga
     1501 acaagttcgc ggaggactgg tctcccgacg gtcgctccat cacgttcggt tcgaatgccg
     1561 gcgggacgat gcagatctgg cgcgtcccgg ccgccggagg aaaggaggag ccgctcacct
     1621 ccgagcctga gcggatgcgg catgcctcgt actcgcctga cggccgcacg atctacgtgc
     1681 agcccagcca ccggaacatc gtccgcattc cggccgacgg cggcgccatg accccggtca
     1741 cgactttccc ggagtcgagc ctgttcatcg aggagccggt cgtctccccc gacgggaggg
     1801 cgctcgccta ctgccgcagc catggcggat cgtcgctctg gttcctgacg atcggaaccg
     1861 ggtcgaaggg gggcgcgaag taggagatca gaacggctcg acgccgacat acgagccggc
     1921 ggcgatcacg tggttccaga agtcctcgcg atcctcggtg aacgccttcg tgaacagctc
     1981 ctcggcgcgg gcgcgatccc ctttgaggtt gcaccatgcg ccggcctcga ccgcgatccc
     2041 gccatcccca ggcttctctt tgtgggcccg ctcgtagtac tccttcgcct tcgcccactc
     2101 ccccgcggcg gcgtaggtcg cggcgatcct ccggaggtcg ctcggcaccg gcttcgccac
     2161 cgagttgaac aggcgctcgc cgcccgcctt gtcccccatg aggtagcgca cgcggccgac
     2221 gccgatccgc tcccacgatc cgctccccgc cagccgctcc gcctcgacga gcagccgctc
     2281 cgccaccgcc tttttgtccc cggagaaatc cagcggcgcg gccggcttct tcgccgcgaa
     2341 gacgatggag ccgccgagag cggccgcggc ccccaccacg gccccgatga gcaatgccgg
     2401 aacgacggtc aggcgaacgt gcgtcatggc tctcctttcc tcgaggccga agcctcaggc
     2461 ttccagctcc ttgaactccc cggcgatccg gccgaacagc cagaagacgg cgattgcggc
     2521 gatcatgtgg aggcccatca cgtcccacac ccgcccgggc gccccgctct gcacgaagtg
     2581 attcgacagc gacgtgtaga tgaagccgct ggtcaaccca ccgatcatgg tcgcgaggaa
     2641 attcgatccc atgtagagac ccgccttgtc cttcggcgcg atccaggtga tgtattcctg
     2701 gatgcgcggc gagcagatca tctcgccgat ggcgaacagc gtgattccca ggaggacgat
     2761 cgacggcggc ccgccggcgg cgcgcccgat gacgaagaag ccgatccccg cgatgaacag
     2821 tcccagcatg aaggccggca tggcgcggaa gcgctccgcc gccttcgaca cgaagacctg
     2881 gaacaccatg atgacccagc cggtgtgggc gatcgtctcg ccgaggatgc ggcgcacgcc
     2941 gtcctcccct tcgtgcgaca ggaacccggc gatgccggag cccagcacgc tgctcagatc
     3001 ctggtacagc cgcgccgtgt cgaggttgct gtcgatgtag agggcgcaca ggttgaagaa
     3061 ggcccagaac ggcagccaga agaagaagcc gaggagtatg aggaacgtgg agaatttgag
     3121 atccgacagc gtcacgccga tctcccggag cgtctcgccg accgatttgc cgcgcgtttc
     3181 ccggggcggc tccttgtaga agaagatcgt gatgatcagc atgatgacga tcgagacggc
     3241 cgccgccgcg aatgcgtagt tccaggagat ggcgcgcagg taacccgcca cgatcgggcc
     3301 gaacgagccg ccgatgttca ccatggcgta gaagatcccg aagccgagcg tcttgttggt
     3361 cgagtcggtc gtcacccgca cggttccggc gatgagcggc ttgaagatcc cggccgccag
     3421 cccgatggag agcatcgtca gcgcgatccc cgagagggag cgcgtcgcca ggagcagcag
     3481 gatcgacggc aggtacgcca ggtacgagac gatcagcacc ttcttgaagc cgaagcggtc
     3541 ggcgaacgac cccgagatga ccgggatgcc gtacgacacg cagagaaaca ggctctgcac
     3601 gatccccagc tggtcgcgcg tgtagccgag gtgggtcatg tagatgccga acccgaagta
     3661 gatgccgtaa taggcgaacc gctccagcac ctcgatcgcg ttggccaccc agaagacggg
     3721 cgggaacttg ctcctcgtcg tcgtcatcct cacctccgtt gtgtcatggg tgctcgtcga
     3781 tcagcagata gccgttgccg gtccaggccg cgcgcgcgcc ggcgtcgtcg ttcctcgtgt
     3841 tcagtgtgag aaaacacgcc ttcgcacgca gccgctcgtc cccgcccatc agacacagat
     3901 cggcggcggg gaccaccccg cccccctgcg gagcgaggga tgcgaggcgc tcgaggagcg
     3961 gcgcgaccgg gatccgcagc agcaccacct cgcccgtgag gacctcgacc gcccggccgt
     4021 gctcgtccag ccggcaccag tggcgggagg tcgcgcccgt cggcgtgtcc ctcatgaaat
     4081 agcagtcgta gaggagctcg tatccggcga cgtcgcgcgc cgtatcctgc gtgtggtacg
     4141 agttcgtgtc gggggacgct tcctgctccc agggccggag gtaggcgagc ttcatctcgt
     4201 tcatgaaggc gtcccggatg tcctgcccgt ccttccacgg cttcgcgtgg cccggcggcg
     4261 cgggctcccg catgaggcga tcgagcggcg cgggtccgtg ggaggaggcg aggtagtcga
     4321 cgatggcgct gatctccttc acgtcgtcgg gcggcaccct gtcggcggag ggccgcagct
     4381 tccccgcgac cagaaggccg tgccgtgcca ggagcgcttc gagcctccgc ccctgactgc
     4441 gcagcgagac ctcgaacgtc ccccacgggc cgaacgaggt ggcgagcgcc aggacggcga
     4501 gcgtggcggg gatgaccttg atgctcgggg cccggcggaa cagcatgaac agcgcgacac
     4561 cgacgagcca ggccccgagg atgaacagcc agtaacgtct ctcggtcacc ccgtattggc
     4621 cgacgcgctt cccgagggcg gcgaacagca gccccaggag cggcaggatg gcggcatcga
     4681 agccgcgcgc gaaacgatcg atccagcggt gctcgcgctc cccgcgcccc ggctgcagca
     4741 ggaggatggt cagcacgccg aagaccgacg cgaaggaaac gagccagccg acccatccct
     4801 gcggccactg gcgcgacacc aggatcttcg ccatgtaggc gtacaggatg aggacgtaca
     4861 gcgtgacgag cggcacgagc atgtactggc tgaagatccg cagccccttc gggtgctccc
     4921 ggtccaggtc gagcgccgtc aggtcccgcg gcacccccgc caggaagtgc cagggcagga
     4981 agacgaagac cgagaagaac cacaagcgtg gatagaggga gggattcacc ttcacgtcga
     5041 acaggtgatc gaccgccagg atcgccagcg ccagcccgcc gaagaagaca gcggcgtaga
     5101 gccccgagaa gaagaaccgg aggaagagcc tccggttgaa ctgccagaag ccgttggtct
     5161 ccccgcggcc cagaaagggg gcgatcgcga ccgcgagatc gacggcgagc gtgagctggg
     5221 catagcgcag gaaccccgcg tcccccgtct cgtgatcgag ccagcggcga tagagcagga
     5281 ggagcgcgac gccggcgacg accccgcccc agacggccgc tcgcgacaac ggccggcccc
     5341 agggaggctt ctcctggaac agccgcaggg cgaagaagag cgggatcgcc agcgccagga
     5401 ccatgacgtg gcgcatcggc ccctccccgc cgtcccgatc gttgaagtgg tggatcgaca
     5461 cggccgccgc gagcacgccg gagacgatca cggcggggaa gcgcaggaag gtgctcttcg
     5521 tgtcctgcca gagcgctgcg atcgacgcca gcttcatgga cccccggccc ccccggctct
     5581 gcggtgcgcg acgcggggcg gattctaggc cggatccgtc cgccgtggcc gttgcgaatt
     5641 cccggtcagg ctcctagact gccgccggca tggggggctt cttcgacacc tggatcatcc
     5701 cgatcgtctc ggtgctgttc atcgtcgatc cgctggcggc ggtgccggcc ttcctgacga
     5761 tgaccgacgg cgatccggat gagaagcgcc gcgccaccgc gctcaaggcg gcgatcgtct
     5821 cgaccggcat cctgctgctg ttcgcgaccg cgggaagcgt ggtgctgcgg atcttcggcg
     5881 tgacgctgcc ggccttccgg atcgccggcg gcgtgatcct cgggttgtcg gcgctggaga
     5941 tgctgcgcgc cgagcgcccg agccgggaga cgcccggcga gatccgcgag gggatcgaga
     6001 agccggatat ctcgatcacg ccgctgtcga ttcccctcct ggcggggccg ggcgcgatct
     6061 cgacggtgat ggtgctcgcc ccggcccacc acacctggcg ggcgtcgatc ccggtctacg
     6121 cggcgatcct tctcaccggg ctcgtcacct gggcgctcct gaaccgctcg gcgctgctgc
     6181 agaggatcct gggaacgacc ggcatccacg tcatgagccg cctga
//
LOCUS       DASRIQ010000610         5409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2579677, whole genome shotgun sequence.
ACCESSION   DASRIQ010000610 DASRIQ010000000
VERSION     DASRIQ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2579677"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VFD06_15100"
     CDS             <1..629
                     /locus_tag="VFD06_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZN04911.1"
                     /translation="RRAIQHALDHGQPSVTLVHKGNIMKFTEGAFRDWGYAVARDEFG
                     DVTVTEEESRASGASDGRIVLKDRILDSMFQQLVTRPEDYSVLATTNLNGDYLSYAAA
                     GLVGGLGLAPSANFGDEVAVFEATHGTAPAHAGRDDVNPGALILSGAMLLEHLGWEEA
                     ARLVVDGVRQAIASKRVTYDLARQMVEARTLRCSEFADVVIGRMQAAA"
     gene            661..1587
                     /gene="mdh"
                     /locus_tag="VFD06_15105"
     CDS             661..1587
                     /gene="mdh"
                     /locus_tag="VFD06_15105"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZN04912.1"
                     /translation="MRRKVSIIGAGNVGASAAERIVMRRLADVVLVDIVEGMPQGKSL
                     DLNEAGPVDNYDMRLTGTNGYDATGGSDVVVITSGLPRKPGMSRDDLLAKNFEIVKQV
                     TTEVAKRSPSAVLVIVSNPLDAMAHTALKVSGFPRKRVLGMAGVLDSARMRFFISEAL
                     GVSVADTHAFVLGGHGDTMVPLPRYSTVAGIPITELMPKEKVDAIVQRTRDGGVEVVN
                     LLKSGSAYYAPASSVVQMVQAILLDEHRILPCAAYLEGEYGYKGLFLGVPVMLGDGGL
                     EKVIEIAMTAEEKAALDRSAAAVRELIEKLPL"
     gene            complement(1619..3139)
                     /gene="dacB"
                     /locus_tag="VFD06_15110"
     CDS             complement(1619..3139)
                     /gene="dacB"
                     /locus_tag="VFD06_15110"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZN04913.1"
                     /translation="MPRESRAALLPLVAILLGAAASAAAAPAAAPQPASDLHATQAPL
                     AATLAGLTTSPVLHPQETGIVVMALPERRVIFARQADRPLKPASTLKILTTAAALALL
                     RPEFVHDTPIYADAEIDASGVLAGNLYVQGRGAPDLVGESWWLMARRLAALGLKRVTG
                     NLVADESYFDPVRRPPGWPAPSADSWYNAPQGALSCNFNVVTVRVSPSPFLGGRPDLT
                     LEPASSYFQVLNRATTAAQSTDIRVDRMFEEGQNRLVVGGTVRRGGGTEIVNRSVEEP
                     ALWSLHAFREIARGCGIEVAGRLETGVVPAGAKLLTRHESRPLAALVRDMNKNSNNFM
                     AEMLLKTLGAQFVATPGTTEGGIEMVRAYLAGLGLDPNDLRISDGSGLSDLDRMPAML
                     MAEILARARIDFEIEPELVASLPIGGADGTLSDRFASEEGRRRVRAKTGRVAGAITLA
                     GYASNRDGHVLAFAIFANQPRGTLEAVHRSLDRLVDAMAASRDADLIPAPGAASAQ"
     gene            3248..4417
                     /gene="sucC"
                     /locus_tag="VFD06_15115"
     CDS             3248..4417
                     /gene="sucC"
                     /locus_tag="VFD06_15115"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZN04914.1"
                     /translation="MKIHEYQAKLLLRGHGIDVPRGDVAFSPREAAAQAASLGGRVVL
                     KAQVHAGGRGKGGGIKVAADAVEAAALAEKMIGMTLVTPQTGPQGKKVRRLLVEETLE
                     ITRELYLGITLDRARGIPMVMACAEGGMDIEEVAAKNPGAILLEPVEPLLGLRPFQAR
                     KLAYALGLEGDQVARGVGLISAMARMYEAIDASLVEINPLVVTKSGTLVALDAKMTFD
                     DNALYRHPRIRELRDPDEEEPLEVEASAHSLNYIKLDGSVGCMVNGAGLAMATMDIIH
                     HVGAAPANFLDVGGGASAEQVAAAFRILVADPNVKAVLINIFGGILRVDVLARGLIDA
                     VQKVGVKVPIVVRLEGTNVDEGKRLIAEAKLNVTIADGMLDAAKKVVALAQAGTR"
     gene            4414..5301
                     /gene="sucD"
                     /locus_tag="VFD06_15120"
     CDS             4414..5301
                     /gene="sucD"
                     /locus_tag="VFD06_15120"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZN04915.1"
                     /translation="MSILVDKSSRVVVQGLTGREGSFHARQCMEYGTKVVSGVTPGRG
                     GQTHEGIPVFNTVAEAVSRTGCDVSLIFVPPPAAADAVLEAADAGVRLIVCITEGIPA
                     ADMVRVRPFVEAKGARLIGPNCPGIITPGQCKVGIMPGYIHKPGSVGVVSRSGTLTYE
                     AVAQLTRRGIGQTTCIGIGGDPIIGTTFQDALTLFNADPETRAIVLIGEIGGGMEEEA
                     AAYAKASVKKPIVAFIAGQTAPPGRRMGHAGAIIAGGKGTAADKMAALQGAGATVLKT
                     PAEIGETVERLLGSSGGGR"
     gene            5298..>5409
                     /locus_tag="VFD06_15125"
     CDS             5298..>5409
                     /locus_tag="VFD06_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZN04916.1"
                     /translation="MTERTLSIVKPDGVRQNVVGEVIRRFEAAGLKVVALK"
BASE COUNT          856 a         1836 c         1990 g          727 t
ORIGIN      
        1 tgcgccgggc gatccagcac gccctcgacc acgggcagcc cagcgtcacc ctggtgcaca
       61 aggggaacat catgaagttc accgaggggg cgttccgcga ctggggctac gcggtggcgc
      121 gcgacgagtt cggcgacgtc acggtcacgg aagaagagtc gcgcgccagc ggagcctccg
      181 acgggcgcat cgtcctcaag gatcggatcc tcgactcgat gttccagcag ctggtgacgc
      241 gcccggagga ctactcggtc ctcgccacca ccaacctcaa cggtgactac ctgtcgtacg
      301 ctgccgccgg cctcgtcggt gggctggggc tcgcccccag cgcgaacttc ggggacgagg
      361 tcgcggtgtt cgaggcgacg cacggcaccg ccccggcgca cgccggacgc gacgacgtca
      421 acccgggggc gctcatcctg tccggggcga tgctcctcga gcatctgggc tgggaagagg
      481 cggcgcggct cgtcgtcgac ggggtgcggc aggcgatcgc gtcgaagcgc gtgacttacg
      541 atctggcgcg gcagatggtg gaggcgcgga cgctgcgctg ctccgagttc gctgacgtcg
      601 tcatcggccg catgcaggcg gccgcgtaat catcaacggc acgattccgg gaggacagcg
      661 atgcgcagga aggtctcgat catcggcgcg ggaaacgtcg gcgcctcggc ggcggagcgc
      721 atcgtcatgc ggcgtctcgc cgacgtcgtc ctcgtggaca tcgtcgaggg gatgccgcag
      781 gggaagagcc tcgacctcaa cgaggcgggg ccggtcgaca actacgacat gcgcctgacc
      841 gggacgaacg gctacgacgc caccggcggc tcggacgtcg tcgtgatcac ctccggcctg
      901 ccgcgcaagc cggggatgag tcgcgacgac ctcctcgcga agaacttcga gatcgtcaag
      961 caggtcacga ccgaggtggc gaagcgctca ccgtcagcgg tgctcgtcat cgtcagcaat
     1021 ccgctcgacg cgatggcgca cacggccctc aaggtgagcg gcttcccgag gaagcgcgtc
     1081 ctcgggatgg ccggcgtgct cgactcggcc cgcatgcgct tcttcatctc cgaagcgctc
     1141 ggcgtctcgg tggccgacac ccacgcgttc gtcctcggcg gccacggcga caccatggtg
     1201 ccgctgccgc gctactcgac cgtggccggc atcccgatca ccgagctcat gcccaaggag
     1261 aaggtggacg ccatcgtgca gcggacgcgc gacggcggcg tcgaggtggt gaaccttctc
     1321 aagagcggca gcgcctacta cgccccggcg tcgtcggtgg tgcagatggt gcaggcgatc
     1381 ctgctcgacg agcaccgcat cctgccgtgc gccgcctatc tcgaggggga gtacggctac
     1441 aaggggctct tcctcggcgt cccggtgatg ctgggggacg gcgggctcga gaaggtcatc
     1501 gagatcgcga tgaccgccga ggagaaggcc gccctcgacc gctccgccgc cgcggtgcgg
     1561 gagctcatcg agaagttgcc gctttaggac cccggcccgg aacttccggc gtgccctgtt
     1621 actgcgcgga tgccgctccc ggggcgggga tgagatcggc gtcgcggctg gcggccatcg
     1681 cgtccaccag gcgatcgagc gaacggtgca ccgcctcgag ggtgccacgc ggctgattcg
     1741 cgaagatcgc gaacgccagc acgtggccgt cgcggttcga ggcgtagccg gcaagggtga
     1801 tggcgccggc gacccggccg gtcttggcgc gcacccggcg acgcccctcc tccgaggcga
     1861 agcgatcgct gagcgtgccg tcggcgccgc cgatcggcag cgacgcgacc agctccggct
     1921 cgatctcgaa gtcgatccgc gcccgggcga ggatctccgc catcagcatc gccggcatcc
     1981 ggtcgagatc ggagagaccc gagccgtcgg agatgcgcag gtcgttcggg tcgaggccga
     2041 ggccggcgag gtaggcgcgc accatctcga tacccccttc ggtcgtcccc ggcgtggcga
     2101 cgaactgcgc gccgagcgtc ttcagaagca tctcggccat gaagttgttg ctgttcttgt
     2161 tcatatcgcg cacgagcgcc gcgagcgggc gcgattcgtg gcgcgtcagc agcttcgctc
     2221 cggccgggac gacgccggtc tcgagccggc cggcgacctc gatgccgcag ccgcgggcga
     2281 tctcccgaaa ggcgtgcagc gaccacagcg ccggctcttc gacggagcga ttgacgatct
     2341 cggtcccccc gccgcgccgc accgtcccgc cgaccaccag ccggttctgc ccctcctcga
     2401 acatgcgatc gacgcggatg tcggtggact gcgcagcggt cgtggcgcgg ttgagcacct
     2461 ggaagtacga cgacgccggc tcgagcgtga ggtcgggccg gccgcccagg aacggggagg
     2521 gggagacgcg cacggtgacg acgttgaagt tgcacgacag cgccccctgc ggcgcgttgt
     2581 accaggagtc ggcggagggg gcgggccatc cggggggccg gcgcaccggg tcgaaatacg
     2641 actcgtcggc aaccaggttc cccgtcaccc gcttcagccc gagggcggcg aggcggcgcg
     2701 ccatgagcca ccacgactcg ccgacgaggt cgggggcgcc gcgcccctgc acgtacaggt
     2761 ttccggccag cacccccgag gcgtcgattt cggcgtcggc gtagatcggc gtgtcgtgga
     2821 cgaactcggg gcgcaagagg gcgagcgccg ccgcggtcgt gaggatcttg agggtcgaag
     2881 ccggcttgag cggccgatcg gcctgccggg cgaagatcac cctgcgctcc ggcagcgcca
     2941 tcacgacgat gccggtctcc tgggggtgca gcacgggaga ggtggtgagt ccggcgaggg
     3001 tcgcggcgag cggcgcctgc gtcgcgtgca gatccgacgc gggctggggg gcagcggccg
     3061 gtgcggcggc ggccgacgcg gccgcgccga gcaggatcgc gaccaacggg aggagggcgg
     3121 cgcgggactc tcgcggcatc atcggcagaa gggcggcgga ttctcgcaca gcgcgcaagg
     3181 gctgtcaaaa caagggtttg acacgctccg gaggggatga tagaaagccc tctccccgga
     3241 gggttcggtg aagatccacg aataccaggc gaaactcctg ctgcgcggcc atgggatcga
     3301 cgtcccgcgt ggggacgtcg ccttcagccc gcgcgaggcg gcggcccagg cggccagcct
     3361 cggcgggcgc gtcgtcctga aggcgcaggt gcacgccggc ggccggggca aggggggcgg
     3421 catcaaggtg gccgccgacg ccgtcgaggc cgcggccctc gccgagaaga tgatcggcat
     3481 gaccttggtc acgccccaga ccggcccgca ggggaagaag gtgcggcgcc tcctggtcga
     3541 ggagacgctc gagatcaccc gcgagctgta cctcgggatc accctcgacc gcgcccgcgg
     3601 catcccgatg gtcatggcct gcgccgaagg ggggatggac atcgaggagg tcgcggcgaa
     3661 gaaccccggg gcgatccttc tcgagccggt ggagccgctc ctgggcctcc gccccttcca
     3721 ggcgcgcaag ctcgcctacg cgctcgggct cgaaggggac caggtggcgc gcggcgtggg
     3781 gctcatctcg gcgatggccc ggatgtacga ggcgatcgac gcctcgctcg tggagatcaa
     3841 cccactcgtg gtcacgaaga gcgggacgct cgtcgccctc gacgccaaga tgaccttcga
     3901 cgacaacgcc ctctaccgcc acccgcgcat ccgcgagctg cgcgatccgg acgaggagga
     3961 gccgctcgag gtcgaggcct cggcccactc cctcaactac atcaagctcg acggcagcgt
     4021 cggctgcatg gtcaacggcg cggggctcgc gatggcgacg atggacatca tccaccacgt
     4081 cggcgccgcg ccggcgaact tcctcgacgt cggcggcggc gcctcggccg agcaggtcgc
     4141 ggcggcgttc cgcatcctcg tcgccgaccc gaacgtcaag gcggtcctga tcaacatctt
     4201 cggcggcatc ctcagggtgg acgtgctggc gcgcggcctc atcgacgcgg tgcagaaggt
     4261 cggcgtgaag gtgccgatcg tcgtgcgcct cgaggggacg aacgtcgacg aggggaaacg
     4321 gctcatcgcc gaggcgaagc tgaacgtcac catcgccgac ggcatgctcg acgcggcgaa
     4381 gaaggtcgtg gcgctggcgc aggcggggac acgatgagca tcctcgtcga caagagcagc
     4441 cgcgtcgtcg tccaggggct caccgggcgc gaagggtcct tccacgcccg ccagtgcatg
     4501 gaatacggca ccaaggtcgt ctccggagtg accccggggc gcggcggcca gacccacgag
     4561 gggatcccgg tgttcaacac cgtcgccgag gcggtgtcgc gcaccggctg cgacgtgtcg
     4621 ctgatcttcg tcccgccgcc cgccgccgcc gacgcggtgc tcgaggcggc cgacgccggc
     4681 gtccggctca tcgtctgcat caccgagggg atcccggccg ccgacatggt gcgggtgcgc
     4741 ccgttcgtcg aagcgaaagg ggcgcgcctc atcggcccga actgccccgg gatcatcacg
     4801 ccggggcagt gcaaggtcgg gatcatgccg ggctacatcc acaagccggg atcggtcggc
     4861 gtcgtgtcgc gcagcggcac gctcacctac gaggcggtgg cgcagctgac gcggcgcggc
     4921 atcgggcaga cgacctgcat cggcatcggc ggcgacccga tcatcggcac gaccttccag
     4981 gacgccctga ccctgttcaa cgccgacccg gagacccgcg ccatcgtcct catcggggag
     5041 atcggcggcg gcatggagga ggaggcggcg gcgtacgcga aagcgtccgt gaagaagccg
     5101 atcgtcgcct tcatcgccgg ccagaccgcg ccccccggac ggcgcatggg gcacgccggg
     5161 gcgatcatcg ccggcggcaa ggggacggcg gccgacaaga tggcggccct ccagggggcc
     5221 ggggcgacgg tgctcaagac gccggcggag atcggcgaaa cggtcgagcg cctgctcggc
     5281 tcatccggag ggggacgatg acggaaagga cgctctcgat cgtgaagccg gacggcgtgc
     5341 gccagaacgt cgtcggcgag gtgatccggc gcttcgaggc ggccggtctg aaggtcgtcg
     5401 ctctgaaga
//
LOCUS       DASRIQ010000611         7246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_255999, whole genome shotgun sequence.
ACCESSION   DASRIQ010000611 DASRIQ010000000
VERSION     DASRIQ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255999"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFD06_15130"
     CDS             complement(<1..321)
                     /locus_tag="VFD06_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04917.1"
                     /translation="MSLVILVVAASSSGALAVPDRPVQDLVTRFANLATTSLPRDFVE
                     PEGRTRWDLRALTPRDRFIRNVTDDLVSLRLVDDEPTGPRAWRFVDPDGGDAALRLLR
                     PDRQG"
     gene            complement(372..1205)
                     /locus_tag="VFD06_15135"
     CDS             complement(372..1205)
                     /locus_tag="VFD06_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04918.1"
                     /translation="MRRPAVPGILSAVAGASLALGALSGCAHALKAPPALEEIAGPAP
                     PGDPTDPARLLEQANALFAHRAIADARRAATLALRAAAATTPPSADALLLAIRILVWL
                     AEHEETSEARSLSATRGVQAAQWCDAAASPAPCAYWKAAALGQQARERPSTGLSAVPL
                     VEAGFKEAAAADASLEQGGPDRALALFYLRAPGWPAGPGDPEQGLAHARRAVELSDAY
                     PPNHLALGEALSKTGDASGARREYARARALALRAEEAGDPDAGEWMREADEAIGAAPG
                     T"
     gene            complement(1202..2068)
                     /locus_tag="VFD06_15140"
     CDS             complement(1202..2068)
                     /locus_tag="VFD06_15140"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HZN04919.1"
                     /translation="MLWAMPTITPIVFAAAASLLAATAQAAAATIVFCSPGSPGDTLQ
                     AKPVMEQFARALEKAGGLPAGTVNAEYHEAEAAGVGAMRAQALLGAVPVPFFVAHGKE
                     LGLEPRLSIVTNTGQPERFALVVRKGAIAKSADLSGWEVTGTTGYAAGFVRRLFAKGF
                     GALPDDARITFTAAPLQALRRAASGEKLAVVLDATQSASLASLPFGADLDVLARSEPL
                     PAGLVCVVKPAAAAHAKTVPSLLKGLERMPKSPEGKEALLAIRVERFEPIDAARIAPL
                     RAEEGPAGASRP"
     gene            2140..2216
                     /locus_tag="VFD06_15145"
     tRNA            2140..2216
                     /locus_tag="VFD06_15145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2174..2176,aa:Arg,seq:tcg)
     gene            complement(2222..4063)
                     /locus_tag="VFD06_15150"
     CDS             complement(2222..4063)
                     /locus_tag="VFD06_15150"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN04920.1"
                     /translation="MRIRPDVSTAFLLAGWLVAAASPSLLEAAPPDPSRGSGGMAGAS
                     PGRAGTPAGEKGAKGRADVVLHGGPVYTVDAVRSWAEAVAIKGDKIVYVGGSKGATAW
                     IGPKTKVVDLGGRMLLPAFQDAHIHPISGGTSYSASCALYDFTTKEQYTKKVAEYAAA
                     HRDSAWIRGDGWLLPAFAPTGIPDKRLLDAVVPNRPVYLESKDGHSAWVNSKALEVAG
                     VARDTPDPPGGRIDRDPKTGEAVGGLQDSAMSLVAEKIPPYTVEERRNGLRYALKMLA
                     RYGITSFQDASVGQEELDVYRALDAAGELTARVVASLWWERKEGLEQIPGFLAQRRTY
                     TRGNVRATTIKIMQDGVLEAQTAALLKPYVGKGEQKGLAMVEPDLLKQAVTALDKEGF
                     QVHFHAIGDAAIRQCLDAVEAARRANGARDARHHMAHIQLFEPSDIPRFRTLGVVANF
                     QPLWAFADEYITELTIPFLDADRQRWIYPIGSLLKSGAVVAFGSDWSVSTANPLEELE
                     VAVTRMGPNGETKTPYLPDERIDLRDALAAFTLDAAYVNHQDDRTGSIEVGKLADLIV
                     LDRNLFAIPPGQISDTKVLLTVFGGTPIFGDWSLAAPAASAAPAAGR"
     gene            4166..4837
                     /locus_tag="VFD06_15155"
     CDS             4166..4837
                     /locus_tag="VFD06_15155"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04921.1"
                     /translation="MALFNRSLGAFVILMVLWLASPAVLSTPTKGPAPLPAASFEACA
                     APASPAATPAASGAPVAVAAPVPDVPSCHRLALDLAASRDYERALAVEARLFEREPMN
                     PEVATSLARMQEMGRKDVVQALRMYHEALYAAPGYPAALLGLGSHWKGRGELLIAERY
                     FARGARENPDQPVFQVRLAEVMMEAGRPAEAAPILQDVLARFPESGEADDARRLLSRT
                     SLARP"
     gene            4984..>7246
                     /locus_tag="VFD06_15160"
     CDS             4984..>7246
                     /locus_tag="VFD06_15160"
                     /inference="COORDINATES: protein motif:HMM:NF026035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04922.1"
                     /translation="MSSAVVRRAAVLLSLAFAPLCTAPAAALEECRLLRQPDIQGNTI
                     AFVYAGDLWTVPRAGGVASRLTTHEGLERFPKLSPDGKSVAFTAEYDGNYDAFVVPAE
                     GGEPVRLTFHPDVDVVAEWYPDGKSILLRSQRASAIRRYDRFFKVASGGGFEEMLALP
                     TGGYSTFSADGKKIAFVSPSYDNRTWKRYKGGNAPEIWTYDFAANTSEKITDWPGADE
                     WPMWWRDTVYYASDRGGRTANLWAYDVKTKQHRQVTRFDEYDVKWPSIGGDSIVFENG
                     GHLYVLDLPSEKPVAVKVLVPDDRPATRPELRNVSRFIAGMDLSPSAKRAVFEARGDL
                     FTIPAEKGDMRNLTRSPGWRERDPAWSPDGKWIAYLSDRSGEYQIHVIGSDGKTPDRQ
                     VTKDGATFRYAPRWSPDSKKVAFSDKTMTVWYADVDSGKITKIDKTDFGEVFDYVWSG
                     DSRYVAYSRPERNGMNRVMIYALETGKTWPVSSGLYDDFSPAFDPAGDNLYFISRRTL
                     NPRFGAFELDFQFSATDRIYAAALRTGVPSPVAPQSDEEKGDGADDKDKDKDKKDADK
                     KDGEKKDADKKEDKEKNGKGEESKGDKKEPPKPISIDFEGIGSRIAEIPVPPSRYAGL
                     NVFKGKVLYARIDEPATFGDGDGGAHATLLQYDLEKRKESTLLAGISDYAPSKDGSKV
                     LYQVGETYGIVDAAEGKKTGDGRIDTANLTTVIDPRQEWLQMFNEAWRLERDFFYDPG
                     MGGLDWKAVGERYR"
BASE COUNT         1096 a         2549 c         2568 g         1033 t
ORIGIN      
        1 tccctgccga tcgggccgca gcagccggag ggcggcgtcg ccgccgtccg gatcgacgaa
       61 gcgccaggcg cgcgggccgg tcggctcgtc gtcgacgagc cgcagcgaaa cgaggtcgtc
      121 ggtcacgttg cggatgaagc gatcgcgcgg cgtgagcgct cgcagatccc agcgggtccg
      181 gccttccggt tcgacgaagt cgcgcggcag cgacgtggtc gcgaggttcg cgaagcgggt
      241 cacgagatcc tggacggggc gatccgggac ggcgagcgct ccgctcgacg atgccgcgac
      301 cacgaggatt accagagaca cacgaacatt ccctgcgcgg acggcgatca tcggctttcc
      361 tccagggcgc attatgtccc aggagcggcg ccaatcgcct cgtccgcttc gcgcatccat
      421 tccccggcat ccgggtctcc cgcctcctcg gcgcgcaacg ccagcgcccg cgcccgcgcg
      481 tattcgcgcc gcgctccgga cgcgtcgccg gttttcgaga gcgcttcgcc cagggcgagg
      541 tggttcggcg gatacgcgtc gctcagctcg accgcacggc gggcatgcgc gagcccttgc
      601 tccggatcgc cgggaccggc cggccagccg ggggcgcgca ggtagaagag cgccagcgcc
      661 cggtccggcc ctccctgctc cagcgacgcg tcggccgcgg ccgcctcctt gaagcccgcc
      721 tcgacgagcg gaacggcgga cagaccggtc gatgggcgct cgcgcgcctg ctgcccgaga
      781 gcggccgcct tccagtaggc gcagggagcg ggggaggccg ccgcgtcgca ccactgcgcc
      841 gcctgcaccc cccgggtcgc cgacagcgat cgcgcttcgg acgtctcttc gtgctcggcc
      901 agccagacga ggatccggat ggcgagcagc agcgcgtcgg ccgagggggg cgtggtcgcc
      961 gccgcggcgc gcagcgccag cgtcgccgcg cgccgcgcat cggcgatcgc gcgatgcgcg
     1021 aagagggcgt tggcctgttc gagcagccgg gcggggtcgg ttgggtcgcc gggaggggcg
     1081 gggccggcga tctcctcgag cgccggcggc gccttgagcg cgtgggcgca gccggacaat
     1141 gcgcccaggg caagcgatgc gcccgcgacc gccgacagga ttccggggac ggcgggccgt
     1201 ctcaaggtct ggatgcgccc gcggggccct cttcggcgcg cagcggagcg atgcgggccg
     1261 cgtcgatcgg ctcgaagcgc tcgacgcgga tcgccaggag cgcctccttc ccttcgggag
     1321 acttcggcat ccgctccagc cccttgagca gcgaaggaac ggtcttcgcg tgcgccgcgg
     1381 ccgccggctt cacgacgcag acgagccccg cgggaagcgg ctcggagcga gccagcacat
     1441 cgaggtcggc gccgaagggg aggctggcca gcgatgcgct ctgcgtcgcg tcgagcacca
     1501 ccgccagctt ctcccccgag gcggcgcgac gcagcgcctg caggggagcc gctgtgaacg
     1561 tgatgcgcgc atcgtccggc agcgccccga atcccttggc gaacaggcgg cggacgaatc
     1621 cggcggcgta gccggtcgtg ccggtcactt cccagccgga gaggtcggcc gatttggcga
     1681 tggctccctt gcggacgacg agggcgaagc gctccggctg gcccgtgttc gtgacgatgc
     1741 tgagccgcgg ctcgaggccg agctccttgc cgtgcgcgac gaagaacggg accgggacgg
     1801 cccccagaag cgcctgcgcg cgcatcgccc cgacccccgc cgcttcggct tcgtgatact
     1861 cggcgttgac cgttcccgcg gggaggccgc cggccttctc gagagcgcgc gcgaactgct
     1921 ccatcaccgg cttcgcctgc agcgtgtccc cgggggagcc gggcgagcag aacacgatcg
     1981 tcgcggcggc ggcctgcgcc gtcgcggcca ggagggacgc ggcggccgcg aagacaatcg
     2041 gcgtgatcgt cggcatggcc cagagcatag cgcggatcca gcggggtctt cggagagccg
     2101 aatccggcgt ggtatcgtgc agcgtcacgc cgtcacagcg cgcccgtagc tcagctggat
     2161 agagcaccgg acttcgaatc cggtggtcgg gggttcgaac ccctccgggc gcgccagcgc
     2221 gctatcgacc ggcggccggc gccgcggagg ccgccggcgc cgccagcgac cagtccccga
     2281 agatcggcgt cccgccgaag accgtcagca ggactttcgt gtcggagatc tgcccgggcg
     2341 ggatggcgaa gagattccgg tcgaggacga tcaggtccgc gagcttgccg acctcgatcg
     2401 atccggtgcg atcgtcctgg tggttcacgt aggcggcgtc cagcgtgaag gcggcgaggg
     2461 catcgcgcag atcgatccgc tcgtccggca ggtacggtgt cttcgtctcg ccgttcggcc
     2521 ccatcctggt caccgccacc tccagctcct cgagcgggtt ggcggtcgag accgaccagt
     2581 cgctgccgaa cgccacgacc gcgccgctct tcaagaggga tccgatcggg tagatccagc
     2641 gctggcggtc cgcgtcgagg aacggaatcg tcagctcggt gatgtactcg tcggcgaacg
     2701 cccacagcgg ctggaagttc gcgacgacgc cgagcgtgcg gaagcgcggg atgtcggacg
     2761 gctcgaagag ctggatgtgc gccatgtggt gccgggcgtc gcgcgcgccg ttcgcgcgcc
     2821 gcgccgcctc gaccgcatcg agacactgcc ggatggcggc gtcgccgatg gcgtggaagt
     2881 ggacctggaa cccctccttg tcgagcgcgg tgaccgcctg cttcaggaga tcgggctcga
     2941 ccatcgcgag ccccttctgt tcccccttcc cgacgtacgg tttgagcagc gccgcggtct
     3001 gcgcctcgag cacgccgtcc tgcatgatct tgatcgtcgt cgcccggacg ttcccccgcg
     3061 tgtacgtgcg gcgctgggca aggaaaccgg gtatctgctc cagcccttct ttcctttccc
     3121 accagagcga cgcgaccacc cgcgcggtca gctcgccggc cgcgtcgagc gcccggtaga
     3181 cgtcgagctc ctcctggccg acgctcgcgt cctggaagct ggtgatgccg tagcgggcga
     3241 gcatcttcag ggcgtagcgc aggccgttcc gtcgctcctc gaccgtgtag ggcgggatct
     3301 tctccgcgac gagcgacatg gcggaatcct gcaagccccc gaccgcctcg ccggtcttcg
     3361 ggtcgcggtc gatccgccct ccggggggat ccggcgtgtc gcgggcgacg ccggcgacct
     3421 cgagcgcctt gctgttcacc cacgcggagt ggccgtcctt cgactcgagg tagaccgggc
     3481 ggttcggcac gaccgcgtcg agcagccgct tgtccgggat gccggtcggc gcgaaggcgg
     3541 ggaggagcca tccgtcccct cggatccacg cgctgtcgcg gtgcgccgcc gcgtactccg
     3601 ccaccttctt cgtgtactgc tctttcgtcg tgaagtcgta gagggcgcac gaggcggaat
     3661 acgacgttcc gccggagatc gggtggatat gggcgtcctg gaaggcgggg aggagcatcc
     3721 gcccgccgag atcgaccacc ttggtcttcg gaccgatcca ggcagtcgcc cccttcgatc
     3781 cgccgacgta gacgatcttg tcccccttga tcgccaccgc ctccgcccag gagcggaccg
     3841 cgtccaccgt gtacaccggt ccgccgtgga ggacgacgtc ggcgcgcccc ttcgcccctt
     3901 tctcgccggc cggggtgccg gcccgcccgg gcgacgcgcc ggccatgccg ccggagccgc
     3961 gggacgggtc gggaggagcg gcttcgagga gcgacggtga agcggccgcg acgagccagc
     4021 cagcgaggag aaacgccgtc gagacgtcgg gtcggatgcg catggcacgg ggcctcccgg
     4081 cgccggaggt tacaagactg caatcgcttg cgcaatcgtg agccggggac aatctttcgc
     4141 cacggagagt cgaggaggac ccgccgtggc gctgttcaat cgatccctgg gggcgttcgt
     4201 catcctgatg gtcctgtggc tcgcgtctcc ggccgtcctg tcgacgccga ccaaagggcc
     4261 cgcgccgctc ccggcggcgt ccttcgaggc ctgcgccgca cccgcgtctc cggccgcgac
     4321 gcccgcggcc tccggcgctc cggtcgccgt ggccgcgccg gttcccgacg tcccctcctg
     4381 ccaccggctg gcgctcgatc tggccgcgtc gcgcgactac gagcgcgccc tcgccgtcga
     4441 agcgcgcctg ttcgagcggg agccgatgaa cccggaagtc gcgacctccc tcgcacgcat
     4501 gcaggagatg gggcgcaagg acgtggtgca ggcgctgcgc atgtaccacg aggcgctcta
     4561 cgccgctccc ggttacccgg ccgcactcct gggtctcgga agccattgga aggggcgcgg
     4621 cgagttgctc atcgcggagc gctacttcgc ccgcggggcg cgcgagaatc cggaccagcc
     4681 ggtgttccag gtgcggctcg ccgaggtcat gatggaggcg ggccgtccgg ccgaggcggc
     4741 gccgatcctc caggacgtcc tggctcgttt cccggaaagc ggcgaggcgg acgacgcccg
     4801 acgccttctg tcccgcacca gcctcgcccg gccgtagccc ccttcgccgg gcccccctga
     4861 ttgcgtccca ccgaccgccc ccgcgggcgc cgggaaccga tccgcccgcc gcggcgttgt
     4921 tgacgctgcc ggcgggggta ccctatcctc ccggcttccc gactggagga ggatcctcgc
     4981 gacatgtcat ccgctgtagt tcgccgtgcc gccgttcttc tgtccctcgc cttcgccccc
     5041 ctgtgcaccg caccggccgc cgctctcgaa gagtgccggc tcctgcgcca gccggacatc
     5101 caggggaaca cgatcgcctt cgtctacgcg ggggacctgt ggaccgtgcc gcgcgcgggc
     5161 ggggtcgcgt cgcgcctgac gacgcacgag gggctggagc gcttcccgaa gctgtcgccg
     5221 gacgggaagt cggtcgcctt cacggcggag tacgacggca actacgacgc cttcgtcgtc
     5281 ccggcggagg ggggggagcc ggtgcggctg accttccacc ccgacgtcga cgtggtcgcg
     5341 gaatggtacc cggacggcaa gtcgatcctg ctgcgatcgc agagggcctc ggcgatccgg
     5401 cgctacgacc ggttcttcaa ggtggcgtcg ggcggcgggt tcgaggaaat gctggcgctg
     5461 ccgaccggcg gctactccac tttctccgcc gacgggaaga agatcgcgtt cgtctccccc
     5521 tcctacgaca accggacctg gaagcgctac aaagggggga atgcgccgga gatctggacg
     5581 tacgacttcg ccgccaacac ctcggagaag atcaccgact ggcccggcgc cgacgagtgg
     5641 ccgatgtggt ggcgcgacac ggtctactac gcctccgacc gcggcgggcg caccgccaac
     5701 ctctgggcgt acgacgtgaa gacgaagcag caccggcagg tgacccgctt cgacgagtac
     5761 gacgtgaagt ggccgagcat cggcggcgac agcatcgtct tcgagaacgg cggccacctg
     5821 tacgtcctcg atctcccgtc ggagaaaccg gtcgcggtga aggtgctcgt ccctgacgat
     5881 cggccggcga cccgtccgga gctccgcaat gtctcgcgtt tcatcgccgg catggatctc
     5941 tccccgtcgg ccaagcgtgc ggtgttcgag gcgcgcggcg atctgttcac catccctgcc
     6001 gagaaggggg acatgcgcaa cctgacccga tcccccggat ggcgcgagcg ggacccggcc
     6061 tggtcgccgg acggcaaatg gatcgcctat ctctccgacc ggagcggcga ataccagatc
     6121 cacgtcatcg gcagcgacgg caagaccccg gaccggcagg tgacgaagga cggggcgacg
     6181 ttccgctacg cgccgcgctg gtcccccgac tcgaagaagg tcgccttctc cgacaagacc
     6241 atgaccgtgt ggtacgccga cgtcgacagc gggaagatca cgaagatcga caagaccgac
     6301 ttcggcgagg tcttcgacta cgtctggtcg ggcgactcgc gctacgtcgc ctactcgcgc
     6361 ccggagcgca acggcatgaa ccgcgtgatg atctacgcgc tcgagaccgg aaagacgtgg
     6421 ccggtgtcga gcggcctgta cgacgacttc tccccggcct tcgaccccgc cggcgacaac
     6481 ctctatttca tctcgcgccg gacgctcaat ccccgcttcg gggcgttcga gctcgatttc
     6541 cagttctccg ccaccgaccg gatctacgcg gcggcgctcc gcaccggcgt cccctcgccg
     6601 gtcgcccccc agagcgacga ggagaagggg gacggcgccg acgacaagga caaggacaag
     6661 gacaagaagg acgccgacaa gaaggacggg gagaagaagg acgcggacaa gaaagaggac
     6721 aaggagaaga acggcaaggg ggaggagtcg aaaggcgaca agaaggagcc gcccaagccg
     6781 atcagcatcg acttcgaggg gatcggatcg cggatcgccg agatcccggt cccgccgagc
     6841 cggtacgccg ggctcaacgt cttcaaggga aaggtgctct acgcccggat cgacgagccg
     6901 gcgaccttcg gcgacgggga cggcggtgcg cacgccacgc tcctgcaata cgacctcgag
     6961 aaacggaagg agtcgacgct gctcgccggc atctcggact acgcgccgag caaggacggg
     7021 tcgaaggtcc tctaccaggt cggcgagacg tacggcatcg tcgacgcggc ggaagggaag
     7081 aagacgggag acggccggat cgacaccgcc aacctgacca cggtcatcga cccgcgccag
     7141 gaatggctgc agatgttcaa cgaggcctgg cgccttgagc gggacttctt ctacgacccc
     7201 ggcatggggg ggctcgactg gaaggcggtg ggggaacgct accgcc
//
LOCUS       DASRIQ010000612         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3119308, whole genome shotgun sequence.
ACCESSION   DASRIQ010000612 DASRIQ010000000
VERSION     DASRIQ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119308"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /gene="mrdA"
                     /locus_tag="VFD06_15165"
     CDS             <1..1311
                     /gene="mrdA"
                     /locus_tag="VFD06_15165"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZN04923.1"
                     /translation="LFDLGDIVGKAGLERQYETSLHGIKGWKEVVVNSHGREMQEVGE
                     GRPPSSGHSLRLTIDLELQRILEEAYGQELGSAVFLDAKTGAVLAMISRPAYDPNVFA
                     HRFSEDVWQSLVRDPRHPLQDRVSLSKFAPGSVFKVVMTIAALEEGVARPERVDHCTG
                     SYRFGERTFQCWGIRKGGHGWVDMRQALIESCNIYFYRLGNEMGIERIARWAHLLGFG
                     QATGIDLPHEESGTVPDAPWKARAVPRDPKWHPGETISVSIGQGALEVTPLQMAVFAA
                     TVGSGGTVLRPHLVQAREAREGVEQGNVDDVVVRRVELHPNTLKVVKEAMWGVVNEAG
                     GTGGRARLEGYDVCAKTGTAQVFKVSRDIDADKMAKEKRDHAWFVGFAPKDDPQIAFA
                     VFVQNGGHGGTVGAPIARAVLERFFAQREGIKGGLSVASAAPPR"
     gene            1283..>2040
                     /locus_tag="VFD06_15170"
     CDS             1283..>2040
                     /locus_tag="VFD06_15170"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZN04924.1"
                     /translation="MSQARRHLGSFDGLLFFSALALQIIGLVVIYSSTTGTPSAGAFQ
                     RQAMWMALGMAGLLVVLLIDYHTLAEFAVVFYAVSLVLLVATLVFGRVVNASKSWLGI
                     GGMQFQPSELAKVATILMVAAYLGREQVRGLGFVRFAAVAGLVGLPVLLIMQQPDLGT
                     AATFMPLLLGSAFLAGLRIRTIVILVLIAALALPIIWGHLKPYQKERVKIFLEPTRDP
                     KGAGYQLIQSLIAVGSGGVAGKGFLSGTQGQLQFL"
BASE COUNT          292 a          672 c          739 g          337 t
ORIGIN      
        1 ctgttcgatc tcggcgacat cgtcggcaag gcggggctcg agcggcagta cgagacgagc
       61 ctgcacggca tcaaggggtg gaaggaggtc gtggtcaaca gccacgggcg cgagatgcag
      121 gaggtcgggg agggtcgccc cccctcctcg gggcactcgc tgcgcctgac gatcgacctc
      181 gaactgcagc ggatcctcga ggaggcgtac ggccaggagc tgggctccgc ggtcttcctc
      241 gacgcgaaga ccggcgccgt cctcgcgatg atctcgcggc cggcctacga cccgaacgtc
      301 ttcgcgcacc gcttctcgga ggacgtctgg cagtcgctcg tccgcgatcc gcgtcatccg
      361 ctccaggatc gcgtctccct ctcgaagttc gcccccggct cggtcttcaa ggtcgtcatg
      421 accatcgccg ccctcgagga aggggtggcc cggccggaac gcgtcgatca ctgcaccggc
      481 tcctatcgct tcggcgagcg caccttccag tgctggggga tccgcaaggg agggcacggc
      541 tgggtcgaca tgcgccaggc gctcatcgag tcgtgcaaca tctacttcta ccgcctcggc
      601 aacgagatgg ggatcgagcg gatcgcccgt tgggcccatc tgctcgggtt cgggcaggcg
      661 accggcatcg acctgccgca cgaggagtcg gggacggtgc cggacgcgcc gtggaaggcg
      721 cgggccgttc cgcgcgatcc gaaatggcac ccgggggaga cgatctcggt gtcgatcggc
      781 cagggggcgc tcgaggtcac gccgctgcag atggcggtgt tcgccgccac cgtcggcagc
      841 ggcggcaccg tgctgcgccc ccacctcgtg caggcgcgcg aggcgcgcga gggggtggag
      901 caggggaacg tcgacgacgt cgtggtgcgg cgcgtagagc tgcacccgaa cacgctgaag
      961 gtggtcaagg aggcgatgtg gggcgtggtc aacgaggcgg ggggaaccgg gggccgcgcc
     1021 cgcctcgaag gctacgacgt ctgcgccaag accggcacgg cccaggtgtt caaggtgtcg
     1081 cgcgacatcg acgcggacaa gatggcgaag gagaagcgcg atcacgcctg gttcgtcggc
     1141 ttcgccccca aggacgatcc gcagatcgcc ttcgccgtct tcgtgcagaa cggcggccac
     1201 ggcggcaccg tgggggcgcc gatcgcccgc gccgtcctgg agcgcttctt cgcgcagcgc
     1261 gaggggatca agggagggct gagtgtcgca agcgcggcgc cacctcggta gcttcgacgg
     1321 gctgctgttc ttctcggccc tggccctgca gatcatcggc ctggtggtga tctattcctc
     1381 gaccaccggc acgccgtcgg ccggcgcgtt ccagcgccag gcgatgtgga tggcgctcgg
     1441 gatggccggc ctgctcgtgg tgctgctcat cgattaccac acgctggcgg agttcgcggt
     1501 cgtcttctac gcggtgagtc tcgtcctgct cgtggcgacc ctcgtcttcg ggcgggtggt
     1561 gaatgccagc aagtcgtggc tcgggatcgg gggcatgcag ttccagccga gtgagctggc
     1621 gaaggtcgcc accatcctca tggtggccgc gtatctcgga cgggagcagg tgcgcggcct
     1681 cgggttcgtg cgcttcgcgg cggtggcggg gctcgtcggc ctgccggtcc tgctgatcat
     1741 gcagcagccg gatctcggga cggcggccac cttcatgccg ctcctcctcg gatcggcgtt
     1801 cctcgccggc ctgcgcatcc gcacgatcgt catcctcgtc ctgatcgcgg cgctggcgct
     1861 gccgatcatc tggggccacc tcaagccgta ccagaaggag cgggtgaaga tcttcctcga
     1921 gccgacgcgc gatcccaagg gggccggcta ccagctcatc cagtcgctga tcgcggtggg
     1981 ctcaggtggt gtcgctggca agggtttcct ctccggcacc caggggcaac tgcagttcct
//
LOCUS       DASRIQ010000613         3244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2580778, whole genome shotgun sequence.
ACCESSION   DASRIQ010000613 DASRIQ010000000
VERSION     DASRIQ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2580778"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VFD06_15175"
     CDS             complement(<1..1234)
                     /locus_tag="VFD06_15175"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HZN04925.1"
                     /translation="MWKRWHLVRDMNLRSSVIWSVVTVDNASFQLPIKRMDVGLEYLR
                     GARQNKAASVSRRARCSSSSSEERMAAWWHRRSVRIAVAAAAALLLYAAAGFLLAPRL
                     LRGPLERETAARLGRDVSLGRLRLNPFRIAMTAERLGIRDPDGTPLVAWERLHVDFDP
                     LRSLWRREWSFREIHLTGATGRLALLPGGALNIDDIITRLTAATPGAAPSGPPPVVSV
                     GRLRIEDTSLAFVDRSGDTPFTTTLGPLRIDLRDFTTRRGEDNAYTFRGGTEAGETFS
                     WSGRFELDPLRSEGEFSLGNVTLAKYHPYYRGVVPFDIRGGTCDLSSRYRFAWGTSER
                     ALALTGASAAIRDLKLSEHDKEEVALETPLAEAQQGEVDLLTGAVSVGRFATQGGHLL
                     LKKSKEGRVNLLDMILPFF"
     gene            1319..2365
                     /locus_tag="VFD06_15180"
     CDS             1319..2365
                     /locus_tag="VFD06_15180"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZN04926.1"
                     /translation="MRTISRAATVFLLILSTGVPPALAGTVSIDGYLEYRKPTYLIVD
                     GQRLEITGATKIKAGKTKRASDIPLGYRIKGKGSRGKDGTIVMTQIEAIKNGSEFMES
                     DILAGTDQAEKSWVEAKKVADAGPDGKEKVIGALIERGPQVDRCRKIVDRLLPSYVDP
                     KQVRVYVVDNPEWNAMAMANFSIYVFSGLMKDMDDDELAIVLGHELAHATYEHSRRQA
                     RAGMASGLAGQAAQIGAGLLENDMMRTAAQQATALGVTTFGNSFSRDYEDQADRVGLR
                     YVYEAGYDYRKAPALWNRFAEKYEDPDKVTNFFFGNHSLSAERAKALQKEIDRNYSDP
                     RKDPPARAASTTAR"
     gene            2492..>3244
                     /locus_tag="VFD06_15185"
     CDS             2492..>3244
                     /locus_tag="VFD06_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04927.1"
                     /translation="MSRARVAGAFLLCSAVWAASTGASRAELPGDVPDKVRIAIGGVA
                     ADAYTDAALSSSSAGVGASINFEDIFDLPENKETLRFELNWRITKRQFIDFGYFELNR
                     SGSRILEEDVEWGDFIFLADGQVTATFDTNFPYAAWRYAFLDLPQVRISGSAGFVYLS
                     ISSGLLASGTVTDINGTPISGEVEEEVSVSAPVPQFGLQLDWALTKRMAMLLYVRQIY
                     ISDIAGIDGGIGESSVRFRWWYAKHAGVSAGLD"
BASE COUNT          561 a         1091 c         1123 g          469 t
ORIGIN      
        1 cgaagaacgg caggatcatg tcgaggagat tgacgcgccc ctccttcgat ttcttcagga
       61 ggaggtgccc cccctgcgtc gcgaaccggc cgaccgacac ggccccggtc aggaggtcca
      121 cctccccctg ctgcgcctcg gcgagcgggg tctcgagggc aacctcctcc ttgtcgtgct
      181 ccgagagctt gagatcccgg atcgcggccg aggcgccggt cagcgccagc gcgcgctccg
      241 aggtccccca ggcgaagcga tagcgggagg agagatcgca ggtgccgccg cggatgtcga
      301 acggcacgac gccgcggtag tacgggtggt acttcgcgag ggtcacgttc ccgaggctga
      361 actccccctc ggatcggagc ggatcgagct cgaagcggcc gctccaggag aaggtctcgc
      421 ccgcctcggt gcccccccgg aaggtgtagg cgttgtcctc gccgcgtcgg gtcgtgaagt
      481 cgcgcaggtc gatgcgcaga gggccgaggg tcgtggtgaa gggggtgtcg ccggaccgat
      541 ccacgaaggc gagcgaggtg tcctcgatcc gcagacgccc gacgctcacg accggaggcg
      601 gcccggacgg cgcggccccc ggggtcgcgg cggtcagccg cgtgatgatg tcgtcgatgt
      661 tgagcgctcc ccccgggagg agggccagcc ggccggtcgc cccggtgagg tggatctccc
      721 taaacgacca ctcgcgccgc cacagcgacc ggagcggatc gaagtcgacg tgaagccgct
      781 cccacgcgac gagcggcgtg ccgtccgggt cgcgaatgcc gagccgctcg gcggtcatcg
      841 cgatccggaa cgggttcagc cgcaggcggc cgagcgagac gtcgcgcccg agccgggccg
      901 cggtctcgcg ctcgagcggg ccgcgcagca ggcgcggcgc gagcaggaag ccggccgcgg
      961 cgtagaggag gagcgccgcc gccgcggcga cggcgatccg aaccgaacga cgatgccacc
     1021 aggccgccat gcgctcctca gatgacgacg aactgcacct ggcgcgtctc gacacgctcg
     1081 ccgccttatt ctgccgcgcc ccccggagat actccaggcc gacgtccatc cgcttgatcg
     1141 ggagctggaa cgaggcgttg tcgacggtca ccacgctcca gatgaccgac gagcgcaggt
     1201 tcatgtcgcg gacgaggtgc cagcgcttcc acatgtgctc gtcgtcgggc gattatatga
     1261 gtggcccctg ccgggagggt ccagattatc atgggccatt cctgagggag agaccgccat
     1321 gcgcaccatc tcgcgcgccg ccaccgtgtt cctgctgatc ctgtcgaccg gtgttccccc
     1381 ggcgctcgcc ggcaccgtct cgatcgacgg atacctggaa taccggaagc ccacgtacct
     1441 catcgtcgat ggccagcgtc tcgagatcac cggcgcgacc aagatcaagg ccggcaagac
     1501 gaagagagca tccgacatcc cgctcggcta ccgcatcaag gggaagggga gccgcggcaa
     1561 ggacggcacc atcgtcatga cccagatcga ggcgatcaag aacggctcgg agttcatgga
     1621 gagcgacatc ctggcgggga ccgaccaggc ggagaagtcg tgggtggagg cgaagaaggt
     1681 cgccgacgcc ggcccggacg gcaaggagaa ggtcatcggc gcgctcatcg agcgcgggcc
     1741 ccaggtggac cggtgccgga agatcgtcga ccggctgctc ccctcctacg tcgatccgaa
     1801 gcaggtgcgg gtctacgtcg tggacaaccc ggagtggaac gccatggcga tggcgaactt
     1861 ctcgatctac gtcttcagcg gtctgatgaa ggacatggac gacgacgagc tggccatcgt
     1921 gctgggacac gagctcgccc acgcgaccta cgagcacagc cgccggcagg ccagggccgg
     1981 catggcgagc ggcctcgccg ggcaggcggc ccagatcggc gcggggctgc tcgagaacga
     2041 catgatgcgg acggccgccc agcaggccac cgcgctcggg gtcaccacgt tcggcaactc
     2101 cttcagccgg gattacgaag accaggcgga tcgggtcggc ctgcgctacg tctacgaagc
     2161 cggatacgac taccgcaagg cgccggcatt gtggaaccgc ttcgcggaga aatatgagga
     2221 cccggacaag gtcaccaatt tcttcttcgg caatcattcg ctgtcggccg agcgggcgaa
     2281 ggcgctgcag aaagagatcg atcgcaacta cagcgatccc aggaaggacc cgccggcccg
     2341 cgccgcctcg acgaccgccc gctgagcctc cagcccggcg agcattcgat cgctgggagc
     2401 ccatgctcga ccgcttgcac tatgatgcgg cgggcgaaaa ccgccgctca gggaggccga
     2461 catgatccat gcacctggca gggcgacgcg gttgagcagg gcgcgcgtcg cgggggcgtt
     2521 cctactgtgc tcggccgtat gggccgcctc gacaggcgcg tcccgcgccg agctgcccgg
     2581 cgacgtgccg gacaaggtgc ggatcgccat cggcggggtg gcggccgacg cctacaccga
     2641 cgccgccctc agctccagca gcgccggtgt cggcgcttcg atcaacttcg aggacatctt
     2701 cgatcttccg gagaacaagg agaccttgcg gttcgagttg aactggcgga tcaccaagcg
     2761 ccagttcatc gacttcgggt acttcgagct gaaccgctcc ggctcccgca tcctcgagga
     2821 ggacgtcgag tggggggact tcatcttcct cgccgacggc caggtgaccg ccacgttcga
     2881 cacgaacttc ccctatgccg cctggcgcta tgcgttcctc gatctccccc aggtgaggat
     2941 ctcggggtcc gcggggttcg tctatctcag catctcctcg ggtctcctgg cgtcggggac
     3001 ggtcaccgac atcaacggaa ccccgatcag tggcgaagtc gaggaggagg tcagcgtcag
     3061 cgccccggtg ccgcagttcg gcctgcagct cgactgggcc ctgacgaagc ggatggcgat
     3121 gctgctctac gtgcgccaga tctacatcag cgacatcgcc gggatcgacg gcgggatcgg
     3181 cgagtcgtcc gtccgattcc ggtggtggta cgccaagcac gccggcgtca gcgccgggct
     3241 cgac
//
LOCUS       DASRIQ010000614         6189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3120057, whole genome shotgun sequence.
ACCESSION   DASRIQ010000614 DASRIQ010000000
VERSION     DASRIQ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3120057"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFD06_15190"
     CDS             <1..377
                     /locus_tag="VFD06_15190"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZN04928.1"
                     /translation="LPQLVNVVRGEMNLIGPRPHPVSNHATFMEHIAYYGLRSTVRPG
                     VTGWAQVCYGYANNLDEETEKMRYDLYYIKHRSIWLDFGILLHTAGSLIAGRSTEVLA
                     EDHAGTPLRVIPAVVRHPVVSR"
     gene            374..1459
                     /gene="yvcK"
                     /locus_tag="VFD06_15195"
     CDS             374..1459
                     /gene="yvcK"
                     /locus_tag="VFD06_15195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HZN04929.1"
                     /translation="MSAATGPDPRGPGVLPGFRRIVAIGGGTGLPAVLQGLKAMLFPP
                     GRPPRSALDRARLTAIVTVSDDGGSTGRLRRAYNIPAPGDLRNCLVALSDAPALARLF
                     MVRFNGRGDVSGHSLGNLILAALHDLEGEFGRAVERAADILAIRGRVVPCTTAPVRLA
                     AEFDDGQVVEGESEIPMVRRRIRRLSLRPEAIPAHPAAREAIAAADLVILGPGSLYTS
                     LLPVLLVRDLRRAIARSEARVALVMNLMTEAGETDGYRATQCLQVLRDHAPEIAIDDV
                     LVNNALLPGERMMRYVAAGSAPIGYEADAIRAFGSRPVESDLLSDGPLIRHDPAKLAR
                     ALMALGGADAAHGGWPFQWAAAADRRR"
     gene            1466..2914
                     /locus_tag="VFD06_15200"
     CDS             1466..2914
                     /locus_tag="VFD06_15200"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN04930.1"
                     /translation="MRRGDASLLTEWWRPAPAAQPGRGPAGRAGAEAPSRPPRALPPA
                     PSWAARPAAGRTLDLQAVSAAAPGGTLPFVALTAFSFVLLLAPQQYLPALAPLRLALV
                     AAAIAIGAHVSSAVMSGRPVSIRTREITLVGALGVWSLVTVPFSLWPGGSLALMTGIF
                     FKSLALFGLNANLVTTARRLKVLAWLFALAGVPLALTAMRHAAEGRLAGGSGEAVKRI
                     AGYEAPLTANPNDMALMLNLLLPFAAALAILDRRPLVKAILWGIVVLDAVGVVLTYSR
                     GGFLTLMTLGAILLFDRLKGKWIASLLIVPAVLVAALTLWLVPPSYLQHMETITDYRS
                     DPTGSAQARLADMITATGIALRHPFIGAGLGMNTLALDQARGHPAWKMVHDVYLQYAV
                     DLGLLGAGLFIALYFCCLGSAGKAARGPGGPPGSDAAAIKEYGRALRNGLIAFGVAAI
                     FHPVAYHFYFYGMGGLAVAVGRLSEGSPHAAS"
     gene            2901..4148
                     /locus_tag="VFD06_15205"
     CDS             2901..4148
                     /locus_tag="VFD06_15205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN04931.1"
                     /translation="MPRLKVVQFLTCLDVGGTERHVATLARSLDPARFDVRVACLRDG
                     GPLSEEIEAAGLTIDRYPISRLWGPATWRQQRRFAALLRSERIDIVHAYGLYAIVFAV
                     PVARLAGVKLVIASIRDNGDPWTRAHRRVQRWVCRLAHGILTNAAEVRRRLQEDGYRD
                     EAIAVIRNGVDIERFQPRPRDPVLRKNLGVPADAPLVVCVSRLNPLKGVDYALLAAAR
                     LVRRFPDVHFLIIGDGAERRPLEDLSRRLGLEGRVVFARMRLDVPEILAQASVSIMPS
                     ISEALPNTMLESMAAGAPLVATRVGGTSEVIEHGVSGLLVPPCDPHALEQAIARLLSD
                     PDQATLMAATARARVVEHFSVERKALETAAFYEALLLGRRPRGDDREEESATILPAAS
                     VPAGPLERRRRQLWEMERRESTR"
     gene            4145..5323
                     /locus_tag="VFD06_15210"
     CDS             4145..5323
                     /locus_tag="VFD06_15210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN04932.1"
                     /translation="MSATKLVVETVTDVARLSALGPEWEDLGAGTSLEHPFLTHDWVE
                     TWWEAFGAGRRLHVLVVRDAGRAIAIAPLMRGRVRFCGLPLQSLESIGNDHTPRGGFL
                     VAPDRPDAFDALWEALSRPGRDFDLLLLRQLPGESPTLQAMLRHAARSGWLEGRWRSA
                     ESPHLNPGGAPFKAYLDSLSPKLRAYLRNKERRAQKLGDVVVETVTEEPDLDAALADG
                     LRIEGSGWKGKAGSAIRNGAETRRFYTLLARRAARRDWLRLNFLRIGARRVAFDFTLR
                     YGRRHFLLKSGYDAEYAQVSPSSLLVRTVLERAFDRGDEEFDFLGGPDAWKTAWTRAT
                     RPHDWLYLMPPSLSLRLMHAAKFRLGPRLRRSPVFNAALGAIRGRADHVAARSNREAL
                     "
     gene            5323..>6189
                     /locus_tag="VFD06_15215"
     CDS             5323..>6189
                     /locus_tag="VFD06_15215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN04933.1"
                     /translation="MRERAASEPMDDGTPARGPVAAPNPAGPVVETVTDPDRLRALAP
                     EWEALASEAGLDHPFLTHEWVEAWWEAFGAGRDLHVLVVRDAGRTIAIAPLMRGRIRI
                     CGLPVASLESIGNEHTPRGGFLVAPERRDAFAVLWDRLFRRGRPFKVVVLRQLPGASE
                     TLVTMQGLAARAGWLVGRWRSAESPWLRVAGTDFPAYYETLSSRFRAHLRNKERRARK
                     IGDLEVETVTGEADLEAALADGLRIEASGWKGKAGSAIQSEAATLRFYTLLARRAARR
                     DWLRLNFLRVGGR"
BASE COUNT          746 a         2280 c         2247 g          916 t
ORIGIN      
        1 agctgccgca gctggtgaac gtcgtccgcg gcgagatgaa cctgattgga ccgcgaccac
       61 atccggtgtc gaaccacgcg acgttcatgg agcacatcgc gtattacggg ctgcgttcga
      121 ccgtgcgacc gggagtcacc ggctgggcgc aggtgtgcta cggctacgcc aataacctcg
      181 acgaagagac cgagaagatg cggtacgacc tgtactacat caagcaccgg tcgatctggc
      241 tcgacttcgg catcctgctg cacaccgccg gctcgctcat cgcggggcgc agcaccgagg
      301 tgctggccga ggatcacgcc gggaccccgc tgcgggtcat ccccgcggtg gtgcggcacc
      361 cggtggtgtc gcgatgagcg ccgcgaccgg tccggaccca cgcggcccgg gggtcctccc
      421 cggcttccgg cgcatcgtgg cgatcggcgg cgggaccggc ctgccggcgg tcctccaggg
      481 gctgaaggcg atgctgttcc cgccggggcg gccgccgcgg tccgccctcg atcgcgcccg
      541 cctgaccgcc atcgtcacgg tgtccgacga cggcggcagc accgggcggc tgcgccgcgc
      601 ctacaacatc ccggccccgg gcgacctgcg caactgcctc gtggcgctgt ccgacgcccc
      661 ggccctggcg cgcctgttca tggtgcgctt caacggccgc ggcgacgtct cgggccacag
      721 cctcggcaac ctgatcctcg cggcgctcca cgatctcgag ggggagttcg ggcgcgccgt
      781 cgagcgggcg gcggacatcc tcgcgatccg cggccgcgtc gtcccctgca ccaccgcccc
      841 ggtgaggctc gccgccgagt tcgacgacgg gcaggtggtc gaaggggaat cggagattcc
      901 catggtccgc cgccgcatcc gacgcctgag cctgcgtccg gaggccatcc ccgcccaccc
      961 cgctgccagg gaggccatcg cggcggcgga cctcgtcatc ctcggaccgg ggagcctcta
     1021 cacgagcctc ctgccggtcc tgctggtccg ggacctgcgc cgcgccatcg cgcggtccga
     1081 ggcgcgggtc gcgctggtca tgaacctgat gaccgaggcg ggggagaccg acggataccg
     1141 cgccacgcag tgcctgcagg tgctccgcga tcacgcgccg gagatcgcca tcgacgacgt
     1201 gctcgtcaac aacgccctgc tccccgggga gcggatgatg cgctacgtcg ccgccggctc
     1261 ggcacccatc ggctacgagg cggacgccat ccgcgccttc gggtcccgcc cggtggagtc
     1321 cgatctcctg tcggacggtc cactcatccg ccacgatccg gccaagctgg cgcgcgccct
     1381 gatggcgctc ggcggcgcgg acgcggcgca cggcggctgg ccgttccagt gggcggcggc
     1441 cgcggaccgg cggaggtgag gtgacatgag acgcggcgac gcctccctcc tcaccgagtg
     1501 gtggcggccg gccccggcgg cccagccggg gagaggaccg gccggccgcg ccggcgccga
     1561 ggctccgtcc cgcccccctc gcgccctccc cccggccccg tcctgggccg cccgcccggc
     1621 ggcgggccgc accctcgatc tccaggccgt ttcggcggcg gcgccgggcg ggaccctccc
     1681 gttcgtcgcg ctcaccgcct tctcgttcgt gctgctgctc gcgccgcagc agtacctgcc
     1741 ggcgctggcg ccgctgcgcc tggccctggt ggcggccgcc atcgccatcg gggcgcacgt
     1801 ctcctcggcg gtgatgtccg gccgcccggt gagcatccgg acgcgcgaga tcaccctggt
     1861 cggcgctttg ggcgtctgga gtctcgtgac ggtgccgttc tccctctggc ccggggggag
     1921 cctggcgctc atgaccggca tcttcttcaa gtcgctggcg ctgttcggcc tgaacgccaa
     1981 cctcgtcacg accgcccgcc ggctcaaggt gctggcctgg ctgttcgcgc tcgccggcgt
     2041 gccgctcgcg ctgacggcga tgcggcacgc cgcggaagga cgcctggcgg gcggctccgg
     2101 cgaggcggtg aagcggatcg ccggctacga agcgccgctc accgccaacc cgaacgacat
     2161 ggcgctcatg ctgaacctgc tcctgccgtt cgcggcggca ctcgcgatcc tcgaccggcg
     2221 ccccctcgtg aaagcgatcc tctgggggat cgtggtgctc gacgccgtcg gcgtcgtcct
     2281 cacctactcg cgcggcgggt tcctgacgct gatgacgctc ggggcgatcc tcctgttcga
     2341 ccggctgaag gggaagtgga tcgcctccct cctgatcgtc ccggcggtgc tggtcgccgc
     2401 gctcacgctg tggctcgtcc ctccttcgta cttgcagcac atggagacga tcaccgacta
     2461 ccgctccgac ccgaccgggt cggcgcaggc gcgcctcgcc gacatgatca ccgccaccgg
     2521 catcgcgctg cgccatccgt tcatcggcgc cggtctgggg atgaacacgc tggctctcga
     2581 ccaggcccgc ggccaccccg cctggaagat ggtgcacgac gtctacctcc aatacgccgt
     2641 cgatctcggg ctgctcgggg cggggctgtt catcgccctg tatttctgct gcctcgggtc
     2701 ggcggggaag gcggcgcgcg ggccgggcgg tcctccggga tccgacgccg ccgcgatcaa
     2761 ggaatatggc cgcgccctgc gcaacggcct gatcgccttc ggcgtcgcgg cgatcttcca
     2821 cccggtcgcc taccacttct acttctacgg catgggcggt ctcgccgtcg ccgtcggaag
     2881 actctccgaa gggagcccgc atgccgcgtc ttaaagtcgt gcagttcctc acctgcctcg
     2941 acgtcggagg gaccgagcgc catgtcgcga ccctggcgcg gtcgctcgat cccgcgcgct
     3001 tcgacgtgcg ggtcgcctgc ctgcgggacg gcgggccgtt gtcggaggag atcgaggcgg
     3061 cggggctgac catcgaccgg tatccgatct cccgcctgtg gggccccgcc acctggcggc
     3121 agcagcggcg cttcgccgcg ctgctccgca gcgagcggat cgacatcgtg cacgcctacg
     3181 gcctgtacgc catcgtcttc gcggtgccgg tggcccgcct ggcgggggtg aagctcgtca
     3241 tcgcctcgat ccgcgacaac ggcgacccgt ggacccgcgc ccaccgccgc gtgcagcgct
     3301 gggtctgccg gctggcgcac ggcatcctga ccaacgccgc cgaggtgcgg cgccgcctgc
     3361 aggaggacgg ctaccgcgac gaggcgatcg cggtcatccg caacggcgtc gacatcgagc
     3421 ggttccagcc gcggccgcgc gatccggtgc tccggaagaa cctcggcgtc cccgccgacg
     3481 cgccgctggt cgtgtgcgtg tcgcgcctca acccgctcaa gggggtcgat tacgcgctcc
     3541 tggcggcggc gcggctggtg cggcgcttcc cggacgtgca cttcctgatc atcggcgacg
     3601 gcgcggagcg tcgcccgctc gaagacctgt cgcggcgcct gggcctcgaa gggcgcgtgg
     3661 tgttcgcgcg catgcggctc gacgtgccgg agatcctggc gcaggcctcg gtgtcgatca
     3721 tgccgtcgat cagcgaggcg ctgccgaaca ccatgctcga gtcgatggcg gccggcgcgc
     3781 ccctggtggc gacgcgggtc ggcggcacct ccgaggtgat cgagcacggg gtcagcggcc
     3841 tcctcgtccc cccctgcgat ccgcacgccc tcgagcaggc gatcgcgcgg ctcctgtccg
     3901 acccggacca ggcgaccctg atggcggcca cggcgcgcgc gcgggtcgtc gagcacttct
     3961 cggtcgagcg caaggcgctc gagaccgccg ccttctacga ggcgcttctg ctcgggcggc
     4021 ggccgcgcgg cgacgaccgc gaggaggaga gcgccacgat cctgccggcc gcgtcggtcc
     4081 cggcggggcc gctggagcgg cgccgccgcc agctgtggga gatggagcgc cgggagtcga
     4141 cccgatgagc gcgacgaagc tggtcgtcga aaccgtcacg gacgtcgctc ggttgagcgc
     4201 cctcggcccc gagtgggagg acctcggcgc ggggacgtcc ctggagcacc cgttcctgac
     4261 gcacgactgg gtggagacct ggtgggaggc gttcggcgcc ggccgccggc tgcacgtcct
     4321 ggtggtgcgc gatgccggcc gtgcgatcgc catcgcgccc ctgatgcggg ggcgggtgcg
     4381 gttctgcggt ctgccgctgc agagcctcga gtcgatcggc aacgaccaca cgccgcgcgg
     4441 cgggttcctg gtcgccccgg accgcccgga cgcctttgac gcgctgtggg aggcgctgtc
     4501 gcgcccgggg cgcgatttcg acctcctgct cctgcgccag ctgcccggcg agtccccgac
     4561 gctgcaggcg atgctccgcc acgcggcccg ctccggctgg ctcgaggggc gctggcgctc
     4621 ggcggagtcg ccgcatctga acccgggggg ggcgccgttc aaggcgtacc tcgactcgct
     4681 ctcccccaag ctgcgcgcct acctgcgcaa caaggagcgg cgcgcccaga agctgggcga
     4741 cgtggtcgtc gagacggtga cggaggagcc ggatctggat gccgccctcg cggacgggct
     4801 gcggatcgag ggctcgggct ggaaggggaa ggccggatcg gcgatccgga acggggcgga
     4861 gacgcggcgt ttctacacgc tcctggcacg gcgggcggcc cgtcgcgact ggctgcggct
     4921 gaatttcctg cgcatcggcg cgcggcgggt cgccttcgat ttcacgctgc gctacggacg
     4981 gcggcatttc ctgctcaagt ccgggtacga cgccgagtac gcgcaggttt cgccgtcgtc
     5041 gctcctggtg cggacggtcc tcgagcgcgc cttcgaccgc ggggacgagg agttcgactt
     5101 cctcggtggc ccggatgcct ggaagacggc ctggaccaga gcgacgcgac cgcacgactg
     5161 gctctacctc atgccgccgt cgctgtcgct gcgcctgatg cacgcggcga agttccggct
     5221 ggggccgcgc ctgcggcgct cgcccgtctt caacgcggcg ctcggcgcca tccgcggccg
     5281 cgccgatcac gtcgccgcgc gatcgaatcg ggaggccctc tgatgcgcga gcgcgccgcc
     5341 tccgagccga tggatgacgg gacgcccgcc cgtgggccgg tcgcggcgcc gaacccggcg
     5401 ggcccggtcg tcgagaccgt caccgacccg gaccggctgc gtgcgcttgc tccggaatgg
     5461 gaagcgctcg cctccgaggc ggggctcgat cacccgttcc tgacccatga atgggtggaa
     5521 gcctggtggg aggcgttcgg cgccggccgg gacctgcacg tcctggtcgt ccgcgacgcc
     5581 ggccggacga tcgccatcgc gccgctcatg cgcggccgga tccggatctg cggcctgccg
     5641 gtcgcgagcc tcgagtcgat cggcaacgag cacacgcccc gcggcggctt tctcgtggcg
     5701 ccggagcgcc gggacgcctt cgccgttctg tgggaccgtc tcttccgccg cgggcggcct
     5761 ttcaaggtgg tggttctgcg ccagctgccg ggcgcttcgg agacgctggt gacgatgcag
     5821 gggctcgcgg cgcgggccgg ctggctcgtg ggacgctggc gctcggccga atccccctgg
     5881 ctgcgcgtcg cggggaccga cttcccggcc tattacgaga ccctgtcctc gaggttccgc
     5941 gcgcacctgc gcaacaagga gcgacgcgcc cggaagatcg gcgatctcga ggtggagact
     6001 gtcacagggg aggccgatct cgaggcggcc ctcgcggacg ggctgcgcat cgaggcgtcg
     6061 ggatggaagg ggaaggccgg ctcggcgatc cagagcgagg cggcgaccct gcgcttctac
     6121 acgctcctgg cgcgccgggc ggcgcggcgc gactggctgc ggctgaactt cctgcgcgtc
     6181 ggcgggcgc
//
LOCUS       DASRIQ010000615         4956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3352935, whole genome shotgun sequence.
ACCESSION   DASRIQ010000615 DASRIQ010000000
VERSION     DASRIQ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3352935"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFD06_15220"
     CDS             complement(<1..514)
                     /locus_tag="VFD06_15220"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HZN04934.1"
                     /translation="MRILRDWGIGFGALVAVIGLILQSVAPERRLWTLSITGFGALLF
                     LAGVVLNARSIGAALRGRRARAASASLGYTLTVLAVVVLANFLATRHHKRIDLTENRD
                     FSLSEQTAKVLEGLPREVTLTSFSRDGDPGRQKLQDLLDEYTYRTRKLTVRYVDPDKN
                     PAEVKRYNVTE"
     gene            complement(528..1370)
                     /locus_tag="VFD06_15225"
     CDS             complement(528..1370)
                     /locus_tag="VFD06_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN04935.1"
                     /translation="MRNTLAIAAREVRTYFTSPLAYVVIGVFLLLSGYIFWASLVRFS
                     ELCLRFASNPYYSSQLNVNDMVVRPIFGTMGIIFLLLLPVVTMRLLAEERRTGTAELL
                     FTCPVTSGQVVLGKFLGAACLFLTMIGVTVTYPILIMNSGAAPDMKPTLAGYFGVLLM
                     GLSLLALGLLFSALTDNQVVAAVSAFGTFLFLWVIDWMASSATVTLGALMSRLTLGLW
                     EKFGLGTAGPTLGDLLGSLSIIGHLRDFQKGVIDTRDVVFYASVIFMSLFLTQRVVES
                     RRWR"
     gene            complement(1367..2371)
                     /locus_tag="VFD06_15230"
     CDS             complement(1367..2371)
                     /locus_tag="VFD06_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN04936.1"
                     /translation="MLEVSDITKTYGARTVVDRVSFSVPKGEILGFLGPNGAGKTTTM
                     RIITGYVPPSSGSAKVAGFDVQDQPLEVKRRLGYLPEHPPLYPEMLVRPYLEFVARIR
                     GVPRKQIRPRVDRAIERCGLQAVAGRLIAVLSKGFQQRVGLAQAILHEPDLLILDEPT
                     VGLDPTQIREIRSLIKSFGGDHTVILSTHILAEVTMTCHRVLIINEGRIAARDTLEGL
                     SARGGGGRRVLVRFARPADGAEAGLRALHGVAAVEAQDGGLVVEMRSGTDPREEIAAL
                     AVGRGWGLVEMRPITLSLEDIFIRIIRGEDAAVAAGPEGPAVQAGGPEGPAVEEGPAA
                     "
     gene            complement(2506..3198)
                     /locus_tag="VFD06_15235"
     CDS             complement(2506..3198)
                     /locus_tag="VFD06_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04937.1"
                     /translation="MRPPAILLGLAAVLLLGGGALFLRTLARLVRTIRARYTYGAPYR
                     EGQIGDRLLGFVMTWPLPALGCACAFLALAQAAFQPIGGVDPVRVGRLDAKRAAWGRT
                     AVRLEPDAAYPSTRGLEGEIEGARWAVEGAFIDWDPGVRWLGLVPGHRVRALLGTRDP
                     SGTSPNKALRTTLDVLPRASEQLLRHAGWLPFLKVRRGTSEWYPPAERRLLILYATPE
                     GYVANFIAEAGG"
     gene            3244..3459
                     /locus_tag="VFD06_15240"
     CDS             3244..3459
                     /locus_tag="VFD06_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04938.1"
                     /translation="MERCRFVTSWGGVVRCGDEALHDGFCRFHFECYRHGEITERGLI
                     SERLDDQERRRAINFHAIRTSSGPQAA"
     gene            complement(3428..4501)
                     /gene="mltG"
                     /locus_tag="VFD06_15245"
     CDS             complement(3428..4501)
                     /gene="mltG"
                     /locus_tag="VFD06_15245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZN04939.1"
                     /translation="MSRPHLPRRRVFLRAGIAAVLLTGIAFGTIAVRAALWASLSYRD
                     YGAASAIVDIPTGTALKRAVQILEDHGILRRFPYSVVTLQALGRGDELKAGEYEFSRP
                     MTPLEVFDKIERAEVYVHKVTVIEGLRSDEIFALYERAGFGTAEEFGEAFRDTSLLRG
                     LDDYAIDLEGYLFPDTYGLQKGTTPKMIVTRMVARFREVLGPEWVAAVKARGLSVRQA
                     VSMASLIEKETAVDEEDPLVASVFHNRLRKGMRLQCDPTVIFALAMRNAWDGNIRRED
                     LKIDSLYNTYRYAGMTPGPIGNPGAAALRAAADPADTEFLYFVSMNNGRHAFSRTLEE
                     HNGAVWEYQKKPFLKRLAVRRTS"
     gene            complement(4530..>4956)
                     /gene="ruvX"
                     /locus_tag="VFD06_15250"
     CDS             complement(4530..>4956)
                     /gene="ruvX"
                     /locus_tag="VFD06_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010347259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZN04940.1"
                     /translation="RVMGLDYGERRIGVALSDPLGLTAQPLTTVDRTASLDADLATLR
                     DLARQHAVVRFVIGLPLGLDGTRGERVRATDRFARRLERVSGIPVEAWDERLTSAQAE
                     RALLEADLSRRRRRQVIDRAAAVLILQSWLDAHSGGARL"
BASE COUNT          780 a         1730 c         1780 g          666 t
ORIGIN      
        1 gctcggtgac gttgtagcgc ttcacttcgg ccggattctt gtccgggtcg acgtagcgca
       61 ccgtcagctt gcgggtccgg taggtgtact catcgaggag gtcctgcagc ttctggcggc
      121 ccggatcccc atcgcgggag aaggacgtga gggtcacctc gcgcggcagg ccctcgagga
      181 ccttggcggt ctgctcgctc aacgagaagt cgcggttctc ggtgagatcg atccgcttgt
      241 ggtgccgcgt cgcgaggaag ttggcgagca cgacgaccgc caggaccgtc agggtgtagc
      301 cgaggctggc ggaggcggcg cgcgcccgcc ggccgcgcag ggcggcgccg atcgaccggg
      361 cgttgaggac gaccccggcg aggaacagca gcgccccgaa cccggtgatc gacagcgtcc
      421 agaggcggcg ctccggcgcc acgctctgga ggatgaggcc gatcacggcc acgagcgccc
      481 cgaacccgat gccccagtcg cgcaggatcc tcatgccgcc cgatccctca gcgccaccgg
      541 cgcgactcga cgacgcgctg ggtcaggaag agcgacatga agatgacgct ggcatagaag
      601 acgacatccc gggtatcgat cacgcctttc tggaagtccc gcaggtgccc gatgatcgac
      661 agcgagccca gcaggtcgcc gagcgtcggc ccggccgtcc ccaggccgaa cttctcccag
      721 agtcccaggg tcagccggct catcagcgcc cccagggtca ccgtggcgga cgaggccatc
      781 cagtcgatga cccagaggaa caggaacgtg ccgaaggcgc tgaccgcggc gacgacctgg
      841 ttgtcggtca gcgcggagaa cagaagcccc agcgccagga gcgacagccc catcagcagg
      901 accccgaagt agccggcgag cgtcggcttc atgtccgggg ccgcgccgct gttcatgatg
      961 aggatcggat aggtgacggt gacgccgatc atcgtcagga acaggcaggc ggcgccgagg
     1021 aacttcccga gcaccacctg gccgctcgtc acgggacacg tgaacaagag ctcggcggtg
     1081 ccggtgcgcc gctcctcggc gagcagccgc atggtcacga ccgggaggag cagcaggaag
     1141 atgatcccca tcgtcccgaa gatcggccgc accaccatgt cgttgacgtt gagctgcgac
     1201 gagtaatagg ggttgctggc gaagcgcagg cacagctcgg agaagcgcac gagcgacgcc
     1261 cagaagatgt agccggacag gagcaggaag accccgatga cgacgtaggc cagcggcgag
     1321 gtgaagtagg tgcgcacctc gcgggcggcg atcgccagcg tgttcctcac gccgcgggtc
     1381 cctcctcaac ggcgggccct tccgggccgc ctgcctggac ggcgggcccc tccgggccgg
     1441 cggcgacggc cgcgtcctcg ccgcggatga tgcggatgaa gatgtcctcg aggctcagcg
     1501 tgatcgggcg catctcgacg agcccccagc cgcggccgac ggcgagcgcc gcgatctcct
     1561 cgcgggggtc ggtgccggag cgcatctcca cgacgagccc gccgtcctgc gcctcgaccg
     1621 cggcgacccc gtgcagcgcg cgcagccccg cctcggcgcc atcggccggg cgcgcgaagc
     1681 ggacgaggac gcgccgcccg ccgccgccgc gcgccgacag cccctcgagc gtgtcgcgcg
     1741 ccgcgatgcg tccctcgttg atgatgagga cgcggtggca ggtcatcgtc acctcggcca
     1801 ggatgtgggt cgagaggatg acggtgtggt cgccgccgaa cgacttgatg agggagcgga
     1861 tctcgcggat ctgcgtcggg tcgagcccga cggtcggctc gtccaggatg agcagatccg
     1921 gctcatgcag gatcgcctgc gccagcccga cccgttgctg gaaccccttc gagaggacgg
     1981 cgatcaggcg gcccgcgacc gcctgcaggc cgcagcgctc gatggcgcga tcgacccgcg
     2041 gccggatctg tttgcgcggc accccccgga tgcgggcgac gaactcgagg tacggccgca
     2101 ccagcatctc gggatagagg ggcgggtgct ccggcaggta gccgagccgg cgcttgacct
     2161 cgaggggctg atcctggacg tcgaagccgg ccaccttcgc cgaacccgac gacggcggga
     2221 cgtagccggt gatgatgcgc atcgtcgtgg tcttgccggc gccgttcggg ccgaggaacc
     2281 cgaggatctc ccccttcgga acggagaacg acacacggtc cacgaccgtg cgggccccat
     2341 aggtcttcgt gatgtccgag acttccagca tgtcgtctgt gcgcctgccg cgggttcacc
     2401 cttccggccg ggcgccggag cccgcgacgc gcgcgctatg ctacccaaga aaccggggga
     2461 gtcaagtcgc gcccgcgtcc acgcgcggcg tggccggagg aaaggctagc cgccggcctc
     2521 ggcgatgaag ttggcgacgt agccttcggg agtggcgtag aggatgagca ggcgccgctc
     2581 ggccggcggg taccactcgg atgtcccacg cctgaccttc aggaacggca gccagccggc
     2641 gtggcgcagg agctgctcgc tggcgcgcgg cagcacgtcg agggtggtgc gcagcgcctt
     2701 gttcggagac gtccccgaag ggtcacgtgt gccgagaagc gcccgcaccc ggtggcccgg
     2761 gacgaggccg agccagcgca ccccgggatc ccagtcgatg aacgctcctt ccacggccca
     2821 gcgggccccc tcgatctccc cttcgagtcc gcgcgtgctg ggataggcgg cgtccggctc
     2881 gaggcggacg gcggtccgcc cccaggcggc gcgcttcgca tccagacgcc ccacgcgcac
     2941 cggatcgacg ccgccgatcg gctggaacgc cgcctgcgcc agcgccagga aggcgcaggc
     3001 gcaaccgagc gccggcagcg gccaggtcat cacgaagccg agcagccggt cgccgatctg
     3061 cccctcgcgg tacggcgcgc cgtaggtgta gcgggcccgg atggtccgga cgaggcgcgc
     3121 gagcgtcctg aggaaaaggg ctccgccgcc gagcaggagg accgcggcca gtccgaggag
     3181 gatcgccggc ggcctcatct atactgtcgc ggcgccgcgc ggtgtcgcgg cggcggaggg
     3241 acgatggagc gctgccggtt cgtgacgtcg tggggcggag tggtgcggtg cggcgacgag
     3301 gcgctccacg acggcttttg ccgcttccac ttcgagtgct accggcacgg cgagatcacc
     3361 gagcgcggcc tcatctccga gcggctcgac gaccaggagc ggcggcgcgc catcaacttc
     3421 cacgccatca ggacgtcctc cggaccgcaa gccgcttgag aaacggcttc ttctggtact
     3481 cccacaccgc cccgttgtgc tcctcgaggg tgcgtgagaa ggcgtgccgc ccattgttca
     3541 tcgacacgaa gtacaggaac tccgtgtcgg cgggatccgc cgcggcgcgg agcgccgccg
     3601 cccccgggtt gccgatcggc cccggcgtca ttccggcgta gcggtaggtg ttgtacagcg
     3661 agtcgatctt gaggtcctcc cgccggatgt tcccgtccca ggcgttccgc atggcgagcg
     3721 cgaagatcac cgtcgggtcg cactgcaggc gcatcccctt gcggagccgg ttgtggaaga
     3781 ccgaggcgac cagcgggtcc tcctcgtcca cggcggtctc cttctcgatg agcgaggcca
     3841 tcgacaccgc ctgacgcacc gacaggccgc gcgccttcac cgcggcgacc cactccgggc
     3901 cgagcacctc cctgaagcgg gccaccatcc gggtgacgat catcttcggc gtcgtcccct
     3961 tctgcaggcc gtaggtgtcc gggaagagat acccctcgag gtcgatggcg taatcgtcga
     4021 gcccccgcag gagcgacgtg tcgcggaacg cctcgccgaa ctcctcggcg gtgccgaaac
     4081 cggcgcgctc gtagagggcg aagatctcgt ccgagcgcaa cccctcgatc accgtcacct
     4141 tgtggacgta tacctcggcg cgctcgatct tgtcgaacac ctcgagcggg gtcatcgggc
     4201 ggctgaactc gtactccccc gccttcagct cgtcgccacg gccgagcgcc tggagcgtga
     4261 cgacgctgta ggggaagcgg cggaggatcc cgtgatcctc gaggatctgg accgcccgct
     4321 tgagcgcggt tcccgtcggg atgtcgacga tcgccgaggc ggcgccgtag tcgcggtacg
     4381 acagcgacgc ccagagcgcg gcgcgcaccg cgatcgtccc gaaggcgatg ccggtgagga
     4441 gcaccgcggc gatgccggcg cgcaggaaga cgcgccgccg cggcaggtgc ggccggctca
     4501 cgcgcacacc atccttgggt cgcgcgcgct cataggcgcg ctccgccgga gtgggcgtcg
     4561 agccaggact ggaggatgag gacggcggcg gcgcgatcga tcacctggcg gcggcgcctg
     4621 cggctcaggt ccgcctcgag gagcgcccgc tccgcctgcg ccgaggtcag ccgctcgtcc
     4681 cacgcctcga ccgggatccc ggagacgcgc tcgaggcgcc gcgcgaaccg atcggtggcc
     4741 cggacccgct ccccccgggt gccgtcgaga ccgagcggca ggccgatgac gaaacgcacc
     4801 accgcgtgct ggcgcgccag atcccgaagc gtcgcgagat cggcgtcgag cgaggcggtc
     4861 cggtcgacgg tcgtgagcgg ttgcgccgtc aggccgagcg gatcggagag ggccacgccg
     4921 atgcgccgtt cgccgtagtc gagacccatc acccgc
//
LOCUS       DASRIQ010000616         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2582377, whole genome shotgun sequence.
ACCESSION   DASRIQ010000616 DASRIQ010000000
VERSION     DASRIQ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582377"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1177
                     /locus_tag="VFD06_15255"
     CDS             263..1177
                     /locus_tag="VFD06_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04941.1"
                     /translation="MSGQDRPIRNDFNPDAEWFASPRPSFGRSALALRIGPLRFAFEG
                     LSERQKERFAAQYGPFLDAGPGAPDLTIRMTRAGVETFLTLQEGGSELYRMPTRLDDG
                     ARRWWSYEFAGTLAADRRHAELALVEEEGPRFERGSENFLRALTAGFILERGGLLLHG
                     AAVVRRGRAYVFFGPSGSGKTTVTRLSPDDLVLSDDLTLIVRDEGEFRAAGIPFGMAH
                     HHIPDSNEAFPIAGLHRLAQSKDVRRERLTGAAAVADVVASLPFVLQDGEDPRPPMEI
                     AARLCDSVPLWRLYFRKDDAFWSVIEER"
     gene            1181..1447
                     /locus_tag="VFD06_15260"
     CDS             1181..1447
                     /locus_tag="VFD06_15260"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HZN04942.1"
                     /translation="MDLSTILTKSPDAAFRTYDGQATIVLPSRAQVSVLNEVGSAVWE
                     RIDGRRTLQEILDGILEEFDITRDQALGDLETFVADLRAQGMVS"
     gene            1449..2546
                     /locus_tag="VFD06_15265"
     CDS             1449..2546
                     /locus_tag="VFD06_15265"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN04943.1"
                     /translation="MANALEKLTTKNWNNAVPMSALFELTFVCNHACSFCYNCPTGQK
                     EMNTAEVIDALHKLAEFNVLYLTLTGGEPLVRRDFFEIAAEARRLGFALRIYTNGYLI
                     DEAMAKKIKQIANPLEMEISIHGAKPETHDKLTCVPGSLKRLLAGVGFLRAEGIKVNL
                     KCPVTKDNQWEVLDMERIANELGARIQFDTVITPRDDGDKDPVSLMSSDDFLEKFWSD
                     DAYKTARKEPVPLPRNDAPGEAVCGTGRSGLAIDPYGNIYPCVQLRRKAGNIRELATL
                     RDMWRTSPVLIQVRREAVEVAEQTLKQTKNGDQCGFCMGVADLQLGDPKAIYPQIARN
                     AEFRARAYSAWKEQTGSSAEETGGVLTKCGM"
BASE COUNT          465 a          912 c          918 g          414 t
ORIGIN      
        1 tgcgccggga cccgcgcctg ggcccactgc acaaagatcc ccggtttcgg gccatgatcg
       61 gtctcgagcc cctgcccccg gcgccccaag gatcgaaata atcccgtcgg aacatgggtt
      121 aaccaccgcg taatccccaa cttgtaacag attttgtttg gacacacccc cccggacacg
      181 gtatatttcg accgtttcga agcagccgag gtcaactcaa accttgttca cccatgcgtc
      241 ccgaggcctc gccgccgagg agatgagcgg acaggaccgc ccaatccgga acgacttcaa
      301 cccggatgcg gagtggttcg cgtcgccgcg cccctcgttc ggtcgcagcg cgcttgccct
      361 gcggatcggg ccgctgcgct tcgccttcga aggtctgtcg gagcgccaga aggaacgctt
      421 cgcggcacag tacggtccct tcctcgacgc cggtcccggc gcccccgatc tcaccatccg
      481 catgacccgc gccggggtgg agaccttcct gaccctgcag gaggggggct ccgagctgta
      541 ccggatgccg acgcgcctgg acgatggggc gcggcgctgg tggtcttacg agttcgcggg
      601 gacgctcgcc gcggaccgcc ggcacgccga gctggccctg gtcgaggaag aggggccgcg
      661 cttcgagcgt ggctcggaga acttcctgcg cgccctgacc gccgggttca tcctggagcg
      721 cggcggcctg ctgctgcacg gcgccgcggt cgtgcgccgc ggccgggcct acgtcttctt
      781 cggcccgtcc ggcagcggca agacgacggt gacccggctg tcgccggacg atctcgtcct
      841 gagcgacgac ctgacgctga tcgtccgcga cgagggggag ttccgggccg ccggcatccc
      901 cttcgggatg gcgcaccatc acatcccgga cagcaacgag gcgttcccga tcgccgggct
      961 gcaccgcctg gcgcaatcga aggatgtgcg ccgcgagcgg ctgaccggcg cggcggcggt
     1021 ggcggacgtg gtcgcttcgc tcccgttcgt gctgcaggac ggggaggatc cgcgcccgcc
     1081 gatggagatc gccgcgcggt tgtgcgacag cgtgccgctg tggcgcctgt acttccgcaa
     1141 ggacgacgct ttctggagcg tcatcgagga gcgctgacac atggaccttt cgaccatcct
     1201 gacgaagagc cccgacgccg ccttccgcac ctacgacggg caggcgacca tcgtgctgcc
     1261 gtcgcgcgcg caggtgagcg tgctcaacga ggtcggcagc gccgtgtggg agcgcatcga
     1321 cgggcggcgg actttgcagg agattctcga cggcattctg gaggagttcg atatcactcg
     1381 cgaccaggcc ctcggagacc tggaaacgtt cgtcgccgac ctgcgcgcac aggggatggt
     1441 gagctgacat ggccaacgcc ctcgagaaac tgacgaccaa gaactggaac aacgccgtgc
     1501 cgatgtcggc gctgttcgag ctgaccttcg tgtgcaacca cgcctgctcc ttctgctaca
     1561 actgcccgac cggacagaag gagatgaaca cggcagaggt gatcgacgcg ctccacaagc
     1621 tggcggagtt caacgtcctg tacctgaccc tcacgggcgg cgagccgctc gtccgcaggg
     1681 acttcttcga gatcgccgcc gaggcccgcc ggctcggctt cgccctgcgc atctacacca
     1741 acggctacct gatcgacgag gcgatggcga agaagatcaa gcagatcgcc aacccgctcg
     1801 agatggagat cagcatccac ggcgcgaagc cggagaccca cgacaagctg acctgcgtcc
     1861 cgggctccct gaagcggctg ctggcggggg tcggtttcct gagggccgag gggatcaagg
     1921 ttaacctcaa gtgcccggtc acgaaggaca accagtggga agtcctcgac atggagcgga
     1981 tcgcgaacga gctgggggcg cgcatccagt tcgacaccgt gatcacgccg cgcgacgacg
     2041 gcgacaagga tccggtgtcg ctgatgtcca gcgacgactt cctcgagaag ttctggagcg
     2101 acgacgccta caagacggcc cgcaaggagc cggtgccgct cccgcgcaac gacgcgccgg
     2161 gagaggcggt gtgcggcacc gggcgctccg ggctggcgat cgatccctac ggcaacatct
     2221 acccgtgcgt gcagctgcgc cgcaaggcgg gcaacatcag ggagctggcg acgctgcggg
     2281 acatgtggcg cacctccccc gtgctgatcc aggtgcggcg cgaggcggtc gaggtggcgg
     2341 agcagacgct caagcagacc aagaacggtg accagtgcgg cttctgcatg ggagtggcgg
     2401 atctccagct gggggatccg aaggcgatct acccgcagat cgccaggaat gccgagttcc
     2461 gtgcccgggc gtactccgcc tggaaggagc agaccggaag ttccgccgag gagaccgggg
     2521 gggtcctcac caagtgcgga atgtgagcgg ggggaacccg ctcgcatcgg cagacgaaag
     2581 gtggtgactt ccggcgcgac gccggagatg ccatgaactc cccgagactc cgttcgctcg
     2641 ttttcggtgc gcttctcctc tgcctcgcca tccccggcca gggggcgcct ggcgtccgca
     2701 ccatcccgc
//
LOCUS       DASRIQ010000617         4201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1020012, whole genome shotgun sequence.
ACCESSION   DASRIQ010000617 DASRIQ010000000
VERSION     DASRIQ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020012"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="VFD06_15270"
     CDS             <1..164
                     /locus_tag="VFD06_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZN04944.1"
                     /translation="ARLRSGRTLGTRAQEVALLDEIAQAMRDASICGLGQTASGAIQS
                     AIRKLGAFR"
     gene            161..1150
                     /locus_tag="VFD06_15275"
     CDS             161..1150
                     /locus_tag="VFD06_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN04945.1"
                     /translation="MSGPLRRLDPTRPVDLTIDGRAVRVPEGTTILEACRAEAIDTPT
                     LCYLENLTPVNVCRVCVVEVEGSRVLVPACSRPVEPKMAVQTDSERVRLSRRLVLEFL
                     ASSVDLSCASEEVRGWMSRYGADPSRYGPPAAALPEGERDAARPGHHHAPDAAAAARV
                     AQPVKVDNDLYVRDYGKCILCYKCVEACGADAQNTFAIAVAGRGFDARISTEYATPLP
                     DSACVYCGNCVGVCPTGALMFKSEHDLREQGAWDESRQTVTDTICPYCGVGCTLQLRV
                     QDNNIVRVTSPLDHEVTSGHLCIKGRFGWQFVQNRPGDGEDPGPASGSGGPSR"
     gene            1147..1893
                     /gene="map"
                     /locus_tag="VFD06_15280"
     CDS             1147..1893
                     /gene="map"
                     /locus_tag="VFD06_15280"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZN04946.1"
                     /translation="MTVRTSEDLEGLRHVGRLVAESLTLLEASVRSGITTAEIDLLAE
                     RFFTERGARSGPQRDYSFPGFICISVNDEIVHGIPGPRVVRPGDLVKLDVTAECGGYI
                     ADAAISVPVPPASETALELIACAREAFEAAMQVVRAGAPIVAVGRAVEATADRYGFQV
                     VRELSGHGVGRRVHEPPEVPNWDDPSIRGTLHDGQVIAVEPILAAGPGRALEAGDGWT
                     MTQEHAFLTVHHEHTLVVRDGPPLILTAAR"
     gene            1907..3271
                     /locus_tag="VFD06_15285"
     CDS             1907..3271
                     /locus_tag="VFD06_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04947.1"
                     /translation="MRRPGLAASVLLAAFALGGTALTVLLQPWESLSWSTWPSGYEAA
                     RIARNLSTGAGYASPFLALPGDNFLSDPPDSEDAGLAAPALQEPKPGSAPSAWVTPPY
                     VLLWWLTFSLFGVYTPVAVTAFQILQVALMTCALAIAWRLVARLRGEKAGLLALALLV
                     FYPSSWYFAVGDTHGTALFLVWLMSSLFTLERVVATAPGPWPILHGLSVALAILTEPV
                     SLFFYLWLEVWAGFRLLRREAGGRGWRGGRLLATTGAALLFVVGPWSIRNLIVLGAPV
                     PFKSNLPMEIYYGNNEESAWNLHVGHIRRFPAWNEEERLRLLAVGEPEYGRQCLLRAA
                     MFLHEHPLTVLRLTLQRITYFWSYNPFRTTTWRPLLTLLFHLPLGVWLAAVLLRPRET
                     DWFDRVCLGFFLLFPLLYYATHLMIYRYRFPLEAILLLAAASAWGRLKGWSREPLATP
                     DAAR"
     gene            complement(3163..>4201)
                     /locus_tag="VFD06_15290"
     CDS             complement(3163..>4201)
                     /locus_tag="VFD06_15290"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN04948.1"
                     /translation="VEAIAEEGGLTRAGDRLHLTQSALSHQLKDIEERLGAPLFLRLK
                     RRMVPTAAGERLLASARSVLTEVRTIEESIRRQARRGEGLIRIAVECYTCYHWLPALL
                     APFHRRFPRVEVRIAAEETRRPVAALLEGRLDMALVSPPPRDPRLRLRALFEDEMVVV
                     LPPAHPLASRAAVLPRDLEGENLYLYSSPEESTVMRRVLLPAGVRPRRITQVMLTEAI
                     LELIRAGQGVSVLSSWSVAPFVSSGRLVVRPLTSRGLRRRWHSARLAAAPEPEWLLAF
                     EDLLARRPPVARLSRRSLRPAAPGARNHAGAKQVQRAASGVASGSRDHPFRRPQADAA
                     ARSRIASSGKR"
BASE COUNT          548 a         1518 c         1517 g          618 t
ORIGIN      
        1 tggcgcgcct tcgctctggc cggacgctcg gcacgcgcgc gcaggaggtg gcgctcctcg
       61 acgagatcgc ccaggcgatg cgggacgcgt cgatctgcgg cctcggccag accgcctcgg
      121 gggcgatcca gtcggcgatc cggaagctcg gggcgttccg atgagcggcc cgctgcgacg
      181 cctcgatccg acgcggccgg tcgacctcac gatcgacggc cgcgccgtgc gcgtgccgga
      241 ggggacgacg atcctcgagg cctgccgcgc agaggcgatc gacacgccga ccctctgtta
      301 tctcgagaac ctgacgccgg tgaacgtctg ccgcgtctgc gtcgtcgagg tcgaggggtc
      361 gcgggtgctg gtgccggcct gctcccgccc ggtcgagccg aagatggccg tccagaccga
      421 cagcgagcgg gtgcggctga gccggcggct ggtgctggag ttcctggcct cctcggtcga
      481 tctctcctgc gcctcggagg aggtgcgcgg ctggatgtcc cgctacggcg ccgacccgtc
      541 ccgctacgga ccgcccgcgg cggctctgcc ggaaggggag cgggacgcgg cgcggccggg
      601 acaccaccac gccccggacg ccgcggcggc ggcgcgggtg gcgcagccgg tgaaggtgga
      661 caacgacctg tacgtccgcg actacggaaa gtgcatcctc tgctacaagt gcgtcgaggc
      721 gtgcggcgcc gacgcgcaga acaccttcgc catcgcggtc gcggggcgcg gtttcgacgc
      781 gagaatctcg accgagtacg cgaccccgct gcccgactcc gcctgcgtct actgcggcaa
      841 ctgcgtcggc gtctgcccga ccggcgccct gatgttcaag agcgagcacg acctccgcga
      901 gcaaggggcg tgggacgagt cgcgccagac ggtgaccgac accatctgcc cctactgcgg
      961 cgtcggctgc acgctgcagc tgcgggtgca ggacaacaac atcgtgcgcg tgacgtctcc
     1021 cctcgatcac gaggtgacga gcggccacct gtgcatcaag ggacgcttcg gctggcagtt
     1081 cgtgcagaac cgacccgggg acggggagga tcccggcccc gcctccggct ccgggggacc
     1141 gtcccggtga ccgtccggac cagcgaggac ctcgaagggc tgcggcacgt cggtcgtctc
     1201 gtggcggaga gcctcacgct cctcgaggcg tcggtgcgct ccgggatcac gacggcggag
     1261 atcgacctcc tcgccgagcg tttcttcacc gagcggggcg cccgctccgg cccgcagcgc
     1321 gactactcct tcccggggtt catctgcatc agcgtcaacg acgagatcgt ccacgggatc
     1381 cccgggccgc gcgtcgtgcg gccgggagac ctggtgaagc tcgacgtgac ggcggagtgc
     1441 ggcggctaca tcgccgacgc ggcgatctcg gtgccggtgc cgccggcgag cgaaacggcg
     1501 ctcgagctca tcgcctgcgc ccgcgaggcc ttcgaggcgg cgatgcaggt ggtgcgcgcg
     1561 ggggcgccga tcgtcgccgt cggtcgcgcg gtggaggcga ccgccgatcg ctacgggttc
     1621 caggtggtgc gggagctctc tggccacggc gtcggccgcc gggtccacga gccgccggag
     1681 gtgccgaact gggacgaccc ctccatccgc ggcacgctgc acgacggcca ggtgatcgcc
     1741 gtcgagccga tcctcgccgc cggccccggg cgcgctctcg aggcggggga cggctggacc
     1801 atgacgcagg agcacgcctt cctcacggtg catcacgagc acacgctcgt ggtgcgcgac
     1861 ggcccgccgc tcatcctcac cgctgcgcgg tgacccccgg acggtcgtga gacggccggg
     1921 cctcgcggct tcagtcctcc tcgccgcgtt cgcgctgggg gggaccgccc tcaccgttct
     1981 gctgcagccg tgggagagcc tctcctggtc gacctggccg agcggctacg aggcggcgcg
     2041 catcgcccgg aatctttcca ccggggccgg ctacgcctcg ccgttcctgg cgctgcccgg
     2101 ggacaacttc ctgtcggatc cgcccgacag cgaggacgcc ggcctcgccg cccctgcgct
     2161 ccaggagccg aagccgggga gcgcgccgag cgcctgggtc actccaccgt acgtgctcct
     2221 gtggtggctg accttctcgc tgttcggggt ctacacgccg gtggcggtga cggcgttcca
     2281 gatcctccag gtggcgctca tgacctgcgc cctcgcgatc gcctggcggc tcgtcgcgcg
     2341 cctgcgcgga gagaaggccg ggctcctggc gctggcgctc ctcgtcttct acccgtcgag
     2401 ctggtacttc gccgtcggcg acacgcacgg cacggcgctg ttcctggtgt ggctgatgag
     2461 ttcgctcttc accctcgagc gcgtggtggc gaccgcgccg ggaccctggc cgatcctgca
     2521 cgggctgagc gtggcgctgg cgatcctcac cgagccggtc tccctcttct tctacttatg
     2581 gctcgaggtc tgggccgggt tccggctcct ccggcgcgag gcgggtggga ggggctggcg
     2641 aggcgggcgg ctgctggcga ccacgggggc cgcgctgctc ttcgtcgtgg ggccctggtc
     2701 gatccgcaac ctgatcgtcc tcggcgcgcc ggttccgttc aagtccaacc tgccgatgga
     2761 gatctactac ggcaacaacg aagagtcggc ctggaacctg cacgtcgggc acatccgccg
     2821 gttccccgcg tggaacgagg aggagcgact gcgtctgctc gcggtcgggg agcccgagta
     2881 cgggcgtcag tgcctgctgc gggccgcgat gttcctgcac gagcacccgc tgacggtgct
     2941 gcgcctgacg ctgcagcgca tcacctattt ctggtcgtac aacccgttcc gcacgaccac
     3001 gtggcggccg ctcctgaccc tcctgttcca cctgccgctc ggcgtgtggc tcgcggccgt
     3061 cctgctccgg ccgcgtgaga ccgactggtt cgaccgggtc tgtctcggct tcttcctgct
     3121 cttccccctc ctttattacg ccacgcacct gatgatctac cgctaccgct tcccgctcga
     3181 ggcgatcctg ctcctcgccg ccgcgtcggc ctggggacgc ctgaaggggt ggtcgcggga
     3241 accgctcgca actccagacg ccgcccgctg aacttgtttc gccccggcgt ggttccgcgc
     3301 acccggcgcc gccgggcgga gactccgcct cgacagccgc gccacgggcg gacgccgggc
     3361 gagcagatcc tcgaaggcca ggagccactc cggctcgggg gcggcggcga gccgggccga
     3421 atgccagcgg cgccgcagcc ccctggaggt gagggggcgc accaccagtc gtccggatga
     3481 cacgaagggg gcgacgctcc acgaggagag gaccgaaact ccctgccccg cccggatcag
     3541 ctccaggatc gcctcggtga gcatcacctg ggtgatgcgg cgcggccgca cgccggccgg
     3601 aagcaggacg cggcgcatca ccgtgctctc ctcgggggag gaatagaggt acaggttctc
     3661 cccctcgagg tcgcggggca ggacggcggc acgggacgcg agcgggtgcg cgggcgggag
     3721 caccaccacc atctcgtcct cgaacaacgc ccgcagacgc agccgtggat cgcgcggcgg
     3781 agggctgacg agcgccatgt ccagccgtcc ctcgaggagg gccgcgaccg gccggcgcgt
     3841 ctcttcggcg gcgatgcgca cctccacgcg cgggaagcga cggtggaacg gagcgaggag
     3901 ggcgggcagc cagtggtagc aggtgtagca ctcgactgcg atccggatga gcccttcccc
     3961 gcggcgcgcc tggcgacgga tcgactcctc gatggtccgc acctccgtca gcaccgaccg
     4021 ggcggaggcg agcaggcgct cgccggcggc ggtggggacc atgcgccgct tgagccggag
     4081 gaacagcggc gcgcccagcc gctcctcgat gtccttgagc tggtggctga gggccgactg
     4141 cgtcaggtgc agccgatcgc cggcgcgcgt cagcccgccc tcctcggcga tcgcctcgac
     4201 c
//
LOCUS       DASRIQ010000618         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_781701, whole genome shotgun sequence.
ACCESSION   DASRIQ010000618 DASRIQ010000000
VERSION     DASRIQ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781701"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFD06_15295"
     CDS             complement(<1..199)
                     /locus_tag="VFD06_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04949.1"
                     /translation="MKTILSVALVLGLCGLARAADEKADPVGTWKCEYAIGDLQRTAT
                     LTIKKDGDKLAGTMSWPDQKEA"
     gene            complement(263..649)
                     /locus_tag="VFD06_15300"
     CDS             complement(263..649)
                     /locus_tag="VFD06_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN04950.1"
                     /translation="MRRVTGIGGIFISASDPEALRDWYRKHLGIDVQPWGGAPFTWTD
                     SDGNPTRGTTVWSIFPADGKHFAPSKSTFMVNYRVEDLAALLNALREEGCDVLEKTDD
                     SEYGKFGWVMDPEGNKVELWQPPPGQ"
     gene            complement(959..>2575)
                     /locus_tag="VFD06_15305"
     CDS             complement(959..>2575)
                     /locus_tag="VFD06_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04951.1"
                     /translation="AVNGNFVLTGLSLLASPKADAAKAQKVSFNKSKATFSQQSYPVE
                     HAVDDERDTGWAIVPQTGRSHTATFFTAAPVGADGGTTLKFQLEQVYGQQHTIGKFRI
                     SVTTDASAADAPSIPAPILAIVKTAADKRTAEQKAQVAAYYRTIDPKLAAEQARAEGL
                     RTAIAPHAEVARLEALLAAQTPQLDAEQARWERAVAAGATWVPVELTGLKSAGGATLT
                     AEPDRSVFAGGANPPTDTYTLTGTSPVRGITAVRLEVLPDPRLPANGPGRGGGNFVLT
                     RFAVASGPKADPSKATPVELHSPAATFEQQGWGPAGLLDERNDTGWAIHPNAGLPAAA
                     TFLTRSQVPGGEDTLLTFTLEHNSQFANHAIGRLRVWVTNALRPDEAPKLPESILAIL
                     RNPNRDDGQKGALATYFRSIAPSLEPVRQRLAELRASAGSTTPAAGRNQGGAIPVLIA
                     RQGGFTGDVKVTLEGFTAGREGAGPRPIDRSLKFSPLVIAGAGTFGTLNFQADANAEQ
                     GTRTVVLRAEAKVGDSAVVQYSPAFPLTVR"
BASE COUNT          333 a          850 c          938 g          454 t
ORIGIN      
        1 tcgcttcctt ctggtccggc cagctcatcg tgccggcgag cttgtccccg tccttcttga
       61 tggtcagggt ggccgtccgc tgcaaatcgc cgatcgcgta ctcgcacttc cacgtcccga
      121 ccgggtccgc cttctcgtcc gccgcgcggg cgaggccgca gagcccgagt accaacgcca
      181 ccgagaggat cgtcttcatg tctgcctccg aaaagtgcat ggtcgcaccg aacttcattg
      241 tcccaatttc attgccccag cttcactgcc ctggtggcgg ttgccagagt tcgaccttgt
      301 tgccctccgg gtccatgacc cacccgaatt tcccgtactc cgagtcgtcg gtcttctcga
      361 gcacgtcgca accttcctcg cgcagtgcgt tgaggagtgc cgccaggtct tcgacccggt
      421 agttgaccat gaaggtcgac ttgctggggg cgaagtgttt gccgtccgcc gggaagatgg
      481 accagaccgt ggttccccgg gtcgggttgc catcggagtc ggtccaggtg aagggtgccc
      541 caccccaggg ctgaacgtcg atcccgaggt gcttcctgta ccagtcgcgc agcgcctcgg
      601 ggtcgctggc gcttatgaag atgccgccga tgcccgtgac ccttcgcact cggacctccg
      661 atgttgggtg actccgtgca ccgtcatccc ggggagcgtc agcgacgagg gatctcggga
      721 tggaacagcc cctcactcca gcccgagatc cctcgggtcg ctaccgcttc cctcgggatg
      781 acggctcaac tgaaacgcgg aactttgcgt gacgagcgga tgttcccgga attcacaatc
      841 ggtctggtct tcctgtcatt tctctcgaaa tgcacgagag atccttcgct gcgctcagga
      901 tgacgattcg acggctgcgc aaccccgacg ggcacctgcg tcgttcgcac caagaacgtc
      961 acctcaccgt cagcggaaac gcggggctgt actgcacgac ggcggagtca cccaccttcg
     1021 cctccgcccg cagcaccacc gtcctcgtcc cctgctcggc gttggcgtcg gcctggaagt
     1081 tgagcgtgcc gaaggtgccg gcgccggcga tgaccagggg gctgaacttg aggctgcggt
     1141 cgatgggccg ggggccggcg ccctcgcggc cggcggtgaa gccttcgagg gtcactttta
     1201 cgtcgccggt gaagccgccc tggcgggcga tcaggacggg gatcgcgccg ccctggttgc
     1261 gaccggcggc gggggttgtc gagccggcgc tggcgcgcag ctcggcgagg cgctgccgca
     1321 ccggttccag cgacggggcg atcgagcgga agtaggtcgc cagcgcaccc ttctggccgt
     1381 cgtcgcggtt ggggttgcgc aggatggcca ggatgctctc ggggagcttg ggcgcctcgt
     1441 cggggcgcag cgcgttggtc acccagacgc gcagccggcc gatggcgtgg ttggcgaact
     1501 gggagttgtg ctcgagcgtg aacgtgagca gcgtgtcctc gccgccaggg acctgcgagc
     1561 gcgtcaggaa ggtggcggcg gcggggaggc cggcgttggg gtggatggcc cagccggtgt
     1621 cgttgcgctc gtcgagcagt cccgccggcc cccacccctg ctgctcgaag gtggcggccg
     1681 gggagtgcag ctcgaccggc gtcgccttcg acgggtcggc cttggggccg ctggcgacgg
     1741 cgaagcgggt gaggacgaag ttcccgccgc cgcggccggg gccgttggcg gggaggcgcg
     1801 ggtcggggag cacttccagg cgcaccgcgg tgatcccgcg caccggcgag gtgccggtga
     1861 gcgtgtaggt gtcggtgggg gggttggcgc cgcctgcaaa gacggagcgg tcgggctcgg
     1921 cggtcagcgt cgcgccgccg gcggatttca ggccggtcag ctccacgggc acccacgtgg
     1981 cgccggcggc gacggcccgc tcccaccgcg cctgctcggc gtcgagctgc ggcgtctgcg
     2041 ccgccagcag cgcctccagc cgcgcgacct ccgcgtgcgg ggcgatggcg gtgcgcagcc
     2101 cctcggcccg cgcctgctcg gcggccagtt tcgggtcgat cgtgcggtag taggcggcga
     2161 cctgcgcctt ctgttccgcc gtgcgcttgt cggcggcggt tttcacgatc gcgaggatcg
     2221 gtgcggggat cgacggcgcg tcggccgcgg acgcgtcggt ggtgacggag atgcggaact
     2281 tgccgatcgt gtgctgctgg ccgtagacct gttcgagctg gaacttcagc gtcgtcccgc
     2341 cgtcggcgcc gacgggggcg gcggtgaaga aggtggcggt gtgcgaccgg cccgtctgcg
     2401 ggacgatcgc ccagccggtg tcccgctcgt cgtccaccgc gtgctccacc gggtagctct
     2461 gctgcgagaa cgtcgccttc gacttgttga aggagacttt ctgcgccttg gcggcgtcgg
     2521 ccttgggcga ggcgaggagg ctcaacccgg taaggacgaa gttgccgttg acggc
//
LOCUS       DASRIQ010000619         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3355306, whole genome shotgun sequence.
ACCESSION   DASRIQ010000619 DASRIQ010000000
VERSION     DASRIQ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3355306"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..500
                     /locus_tag="VFD06_15310"
     CDS             108..500
                     /locus_tag="VFD06_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HZN04952.1"
                     /translation="MSLRVVRLGSPRAPGEGLRIGTVRYPPRGVRKDQYAARDFFDLW
                     LPDLAPSPATVKVAQAATTERQWQGYVKRYRREMAAPERRRLLDLLAALSRHADFSVG
                     CYCEDERRCHRSILRALLEEHGARIANP"
     gene            complement(505..954)
                     /locus_tag="VFD06_15315"
     CDS             complement(505..954)
                     /locus_tag="VFD06_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN04953.1"
                     /translation="MARSSFVYVVYIAATPEAVWKGLTDPESTRQYWGHDNVSDWKPG
                     SAWEHRKADGSGSVSIAGEVIEARKPHRLLLTWSEHQEKRLRHRQSRVLFEIDAVADV
                     VRLTLTHDELEPGSTNERQVAAAWQRVLSSLKTLLETGKPLPAWTGR"
     gene            1040..2071
                     /locus_tag="VFD06_15320"
     CDS             1040..2071
                     /locus_tag="VFD06_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZN04954.1"
                     /translation="MAGHTPSTAPVPPHAQLIQMATGYWVSRIVYAAARLGIADRLAE
                     GPRSAADLAGPTGTHAPALHRLMRTLAALGILTEREAGRFALTPVGEALKTGAPGSAR
                     ASVLTLAGQLGWRSWEEILHSLQTGKTGFEKAFGKPAFDYLADHPEEATLFSETMVGF
                     HGDEPPAVAAAYDFSRFKTVADIGGATGNLLAAILSSHAGPRGLLFDLPHVVKDAPAL
                     LKARGVADRVAFVPGSFFQTIPDGAECYVMSHVIHDWSEEQCLTILGHCRKVVPKDGR
                     LLIVEMVLPPGDGFHPGKLLDITMLVMPGGQERTEGEYAELLGKARFRLERVIPTESA
                     VSIVEAAPA"
     gene            2115..2423
                     /locus_tag="VFD06_15325"
     CDS             2115..2423
                     /locus_tag="VFD06_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HZN04955.1"
                     /translation="MLELRPTCEHCNTALPPDSLEARICSYECTFCAACVERVLENVC
                     PNCGGGFAPRPIRPSRNWKGDNFLGKDPASTKVRHRPVDTAAHARFVAEIKRLAPHER
                     "
     gene            complement(2395..>3118)
                     /locus_tag="VFD06_15330"
     CDS             complement(2395..>3118)
                     /locus_tag="VFD06_15330"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HZN04956.1"
                     /translation="ALVCAARGFPIRIVTSDAFSLEKRDQMRALGADLVLVRSEGGLT
                     TRRLIEEMIETARTISREPRTFWIDQLRNEDSIAGYHPLGEEIWQQTERRVDAFVHSV
                     GTSASLRGAGSVLKEHKPGIRVVAVEPAESPVLSGGQAGPHKIEGVGIGSIPPLWLPS
                     LVDQIVPIPTAEAKEMTRRLAREEGLFAGTSSGANVLAAIQIAKRLGEGSTVVTLMVD
                     SGLKYLGTDVYRSSIARAAPAA"
BASE COUNT          449 a         1108 c         1111 g          450 t
ORIGIN      
        1 ggatcgacga gtcgtaccgg gcgcaggcgc cgaagcgcct cgtcctcctc ctgccagcga
       61 agcccggccg ctgatctccg caggccccgc gctagaatgc cgctcccgtg agcctccgcg
      121 tcgtccggct cggctcgccg cgcgcgcccg gcgaagggct gcgcatcggc acggtgcgct
      181 atccgccgcg cggcgtgcgc aaggaccagt acgccgcgcg cgacttcttc gacctctggc
      241 ttccggatct cgccccctca cccgccacgg tgaaggtggc gcaggcggcc acgacggagc
      301 ggcagtggca ggggtacgtg aagcgctacc gtcgcgagat ggcggcgccg gagaggcgcc
      361 ggctcctgga tctcctcgca gcgctgtcgc gacacgccga cttctccgtc ggctgctatt
      421 gcgaggacga gcgccgctgc catcgctcca tcctgcgggc cctgctcgaa gagcacggcg
      481 cgcggatcgc caatccctga gacgctaccg gccggtccag gcgggaagcg gcttgccggt
      541 ctcgaggagc gtcttgaggc tggagaggac gcgctgccag gcggccgcga cctgccgctc
      601 attcgtcgag ccgggctcca gctcgtcgtg cgtcagcgtc agccggacca cgtcggcgac
      661 cgcgtcgatc tcgaacaaga cgcgcgactg acggtgccgg aggcgcttct cctggtgctc
      721 gctccaggtg agcagcaggc gatgcggctt gcgcgcctcg atcacctcgc cggcgatcga
      781 gacggacccg gagccatcgg ccttgcggtg ctcccaggcg ctccccggct tccagtccga
      841 cacgttgtcg tgcccccagt actgacgggt ggactcgggg tcggtcagcc ccttccagac
      901 tgcctcgggt gtcgcggcga tgtagacgac gtagacaaag ctcgacctgg ccatggtcgg
      961 atccctccgg cccgggcccg tccgggcggg tgctagaatg ccgcaacgtt cccgacgaag
     1021 ccagaaaaaa ggagacgacg tggcaggaca cacgccttcg acggccccag tgccgcccca
     1081 cgcgcagctc atccagatgg cgacagggta ctgggtgtcg cgcatcgtct acgccgcggc
     1141 gcggctcggc atcgccgacc ggctcgcgga agggccgcgc agcgcagccg acctggccgg
     1201 accgaccggc acgcatgcgc cggcgctgca ccggctgatg cggacgctcg cggcgctcgg
     1261 catcctgacc gagcgcgagg cggggcgttt cgccctgacg ccggtcgggg aggcgctcaa
     1321 gacgggcgcc ccgggctcgg cccgggcgag cgtcctgacg ctcgccggcc agctcggctg
     1381 gcgctcgtgg gaggagatcc tgcactcgct gcagaccggc aagaccgggt tcgagaaggc
     1441 cttcggcaaa ccggccttcg actacctggc ggatcacccg gaggaggcga ccctcttcag
     1501 cgagacgatg gtcggcttcc acggcgacga gcctccggca gtggcggcgg cgtacgactt
     1561 ctcgcgcttc aagaccgtgg ccgacatcgg cggcgcgacc gggaacctgc tggcggcgat
     1621 cctgtccagc cacgccgggc cgcgcgggct gctgttcgat ctgccgcacg tggtgaagga
     1681 tgcaccggcg ctcctgaagg cgcgcggcgt cgcggaccgg gtcgcgttcg tgcccggctc
     1741 gttcttccag acgattccgg acggggccga gtgctacgtc atgtcgcacg tcatccacga
     1801 ctggtcggag gagcagtgcc tgacgatcct cggccactgc cgcaaggtgg tgccgaagga
     1861 cggacggctg ctgatcgtcg agatggtcct gccgccgggg gacggcttcc accccgggaa
     1921 gctgctcgac atcacgatgc tggtgatgcc gggcgggcag gagcggaccg aaggggagta
     1981 cgcggagctc ctggggaagg cccgcttccg gctggagcgg gtcattccga ccgagtcggc
     2041 ggtcagcatc gtcgaggcgg ctccggcctg aggacccgaa gcggcgcctg atcgcgcgcc
     2101 gcgcggaggc ggtcatgctc gagctgagac cgacctgcga gcactgcaac acggcgctgc
     2161 cgcccgactc gctcgaggcg cgcatctgct cctacgagtg caccttctgt gccgcgtgcg
     2221 tcgagcgcgt cctcgaaaac gtctgcccga actgcggcgg cgggttcgcg ccgcgtccga
     2281 tccgcccgtc ccgcaactgg aagggggaca acttcctcgg caaggacccg gcgagcacca
     2341 aggtccggca ccgccctgtc gacaccgccg cgcacgcccg cttcgtcgcg gagatcaagc
     2401 ggctggcgcc gcacgagcga tagaggagcg gtagacgtcg gtgccgaggt acttcagccc
     2461 cgagtcgacc atcagcgtaa cgaccgtcga gccttcgccg aggcgcttgg cgatctgaat
     2521 cgccgcgagg acgttggcgc ccgacgaggt cccggcgaac agcccctcct cgcgcgccag
     2581 gcggcgggtc atctccttcg cctcggcggt cgggatcggc acgatctgat cgacgagcga
     2641 cggcagccac agcgggggga tcgagccgat gccgacccct tcgatcttgt gcggccccgc
     2701 ctggccgccg gagaggaccg gcgactcggc cggctcgacc gcgacgacgc ggattcccgg
     2761 cttgtgctcc ttgaggacgc tccccgcgcc gcgcagcgac gccgaggtgc ccacgctgtg
     2821 cacgaaggcg tccacccgtc tttccgtctg ctgccagatc tcctcgccga gcgggtggta
     2881 gccggcgatg ctgtcctcgt tgcgcagctg atcgatccag aaagtgcgcg gctcgcggct
     2941 gatggtccgc gcggtctcga tcatctcctc gatgagccgg cgggtcgtga ggcccccctc
     3001 gctccggacg agcacgaggt cggcgccgag cgcccgcatc tggtcccgct tctcgaggct
     3061 gaaggcgtcg gaggtgacga tgcggatggg gaagccgcgc gcggcgcaga cgagggcg
//
LOCUS       DASRIQ010000620        11647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_485638, whole genome shotgun sequence.
ACCESSION   DASRIQ010000620 DASRIQ010000000
VERSION     DASRIQ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 11647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11647
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485638"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFD06_15335"
     CDS             complement(<1..189)
                     /locus_tag="VFD06_15335"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HZN04957.1"
                     /translation="MTDVANWHVLSTRSQCEQQVHDRLAAEGFEVYLPRTRRWIAPNG
                     EQLMCGAPLFPGFLFLRHL"
     gene            complement(246..776)
                     /locus_tag="VFD06_15340"
     CDS             complement(246..776)
                     /locus_tag="VFD06_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04958.1"
                     /translation="MARLAGIAGMTLLTLGGTKRDLLSAVLEAEAGDRTAGTTSADPV
                     GPRMSGGGSVHASGDQVITHGFELHCDPDALPNSLDIAWEGKRFHLEAVSSSRCADDP
                     AIHPDGPGAPFDTCSGAGSGLYNDEEEATAEWTFTDAGASGVEDRAVMTVRNAAGEIV
                     LEVSGTLTYGNHEALE"
     gene            1127..2296
                     /locus_tag="VFD06_15345"
     CDS             1127..2296
                     /locus_tag="VFD06_15345"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN04959.1"
                     /translation="MLARAPARDAAHYPPLLVAGLSSRRIPGLDGMRALGSFFVVGYH
                     YGLPFVPGGWGVLNFLVLSGFLITWLLLAEEDRKGSIAVRSFYVRRARHIVPAFVVFM
                     IVVAAALLVFGKRIVWPQVITAFLFVVNYYQGLFGDPNTAFSHTWALALQLQFYLLWP
                     LLFLALRRDRRRMAGALLVIIASIWVYRSLMWIGGVVPQGYVYEAFDMRADHLLIGCL
                     MAVLLRTGSMKPVVAVATGSPWMPFLTMALIGVSAACEKHYGVDYRNTLGFIVNPMLI
                     MTLLLQAISFAATPMWRWLEWRWVRYLGMISYSVYLYQQVVVDPVKHRLAFLPAPLQF
                     LGTLAAVYAVSSVAYYLVENPLRGARRQRTQPNVVPRVWAAKVTPERIRPSMWSS"
     gene            complement(2293..3222)
                     /locus_tag="VFD06_15350"
     CDS             complement(2293..3222)
                     /locus_tag="VFD06_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04960.1"
                     /translation="MLVPIAAGLVGGSAHRQIARTFGCAPSTVTRNAAKLGRHAILLL
                     AAVRTELEGQVDERFVLDHFETFEFTQDYPFGVATVVGSKSWYVYDLDPAPHSRTGHM
                     SEPQRRRLGARPRRPSRGGYVGSTRRVFDRLLSLVPPASQAIIHGDGHPAYDRAQRTH
                     PSGRRLRLERYPNPPRGPKGSPRSEVARLRDQRLFPVDLLHKILRHSLAHHRRETIAF
                     GRRLNALLERLFLTAVWRNFVKKRSERVARSGTPAMRLGLARDRWNWPRVFSRRLFPA
                     RIPTPPTWAQIYQRLWTTPLYPVNSRHALKRAF"
     gene            complement(3639..4292)
                     /locus_tag="VFD06_15355"
     CDS             complement(3639..4292)
                     /locus_tag="VFD06_15355"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN04961.1"
                     /translation="MRKSFALVSLLAVAALAFAAPVFVAAALSAPLHADDAPKALAIG
                     TAIPMADQKMKNVDGKEVAVADVKGAKGTLVIFTCNACPYAKAWEDRIVALGNEYKGK
                     GIGVIAVNSNDPGKVAEDGYDQMKTRAKEKSFGFPYVVDSTSNVARAYGATRTPEVFL
                     FDAQGKLVYHGTIDDNSQDPEKVSSRYLSDALTAVSSGKEVPVKETKAMGCGIKFRA"
     gene            complement(4329..4883)
                     /locus_tag="VFD06_15360"
     CDS             complement(4329..4883)
                     /locus_tag="VFD06_15360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN04962.1"
                     /translation="MPLGLCLPLLLAAAPAATAPAAAKPSAQAAAAKPGAKPVLTPAD
                     AAAVKQAITAPGAKAVLVNIWATWCGPCRDEFPDVLKVRKDLEAEGLRVILVSADFVE
                     GKADAVEFLEAMGVDFPSFIKVGKDDEFVNGLAKEWSGLLPATLVYDGKGSLREVREG
                     KQSYATFRGMVEKVLKDGSARSGP"
     gene            5090..5189
                     /gene="ffs"
                     /locus_tag="VFD06_15365"
     ncRNA           5090..5189
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFD06_15365"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5266..7140
                     /gene="dnaX"
                     /locus_tag="VFD06_15370"
     CDS             5266..7140
                     /gene="dnaX"
                     /locus_tag="VFD06_15370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZN04963.1"
                     /translation="MSYQVIARKYRPQTFEEVVGQRPIIQTLQNAVEQRRLHHAYLFS
                     GPRGCGKTTVARLLAKALNCEKGPTATPCNACSSCAEIAQSRSMDVLEIDGASNRGID
                     DVRTLRETVRYHPARDRYKVFIIDEVHMLTDPAWNALLKTLEEPPPQVVFIFATTEYR
                     EIPRTILSRCQHFEFKKVAPSVLLEHLKKVAQGEGVTVEPSAADLIVRVAEGSLRDAL
                     SALDQVIAFSGDKVTDEQARTILGVVDRDLILDFFAAVRGRDCDRIVAIVDTLFEKGY
                     QPVEFLEDVLAQGRDLLLARTVSEPSKYLAGTPEEIRALSERAAAWSEDELLRFIEIV
                     TREETRIKNSSHARFLLEALGIRLARLADLKPIEEILASLEGPGSGAPSPGAKPAGAD
                     PGTRGAAPGAAPGRPPAAPAPGPSRSVSAAPSAEPAAPIPVATEAPVSSPAFVEALLQ
                     RVHEERVAIGSMLEQAIWIQAAEDALRIVFSEKHGFFRDKVQSREVSEYLRSAVRSLS
                     GRDLRIVVETGAPGSFEPMSLGAAAPGMAAPRAAASVATPLNPAAAAPRASVPTAAPA
                     GPPAAPRAGATTGAAPRPATRIIDDAGRQALRDRALQEPAVRSVMDLFGGEIVDVEPL
                     "
     gene            7189..7473
                     /locus_tag="VFD06_15375"
     CDS             7189..7473
                     /locus_tag="VFD06_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019627078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZN04964.1"
                     /translation="MRLLMKQAQKMQEKMQKEIAELQVEGTAGGGMVTIRMTGNHAVA
                     SVSIKPEAVASNDAEMLEDLVLAALNDAHRQIEAALQEKMGSLKIPGFGL"
     gene            7478..8074
                     /gene="recR"
                     /locus_tag="VFD06_15380"
     CDS             7478..8074
                     /gene="recR"
                     /locus_tag="VFD06_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZN04965.1"
                     /translation="MQFAAPLERLIERLRKLPGVGQKSAQRLAFHLLRGDAEEARALA
                     QAIVEVKEKIRLCTICFNITDTDPCLICADLDRDAASLCVVEEPHNLVSIEKSRQFHG
                     RYHILHGSIAPLRGIGPDDLKIGPLLERLRDGTVKEVILATNPNVEGEATAVYLSRLL
                     KPIGVRVTRIALGLPVGSELEYADEVTVGKALEGRRDI"
     gene            8098..10842
                     /locus_tag="VFD06_15385"
     CDS             8098..10842
                     /locus_tag="VFD06_15385"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN04966.1"
                     /translation="MAGVKKGLFLRIEDFARRRYAVVFAVTALLVLASILLGKRLRLD
                     GDILNLVPKGNRVVNTFREALQDFGSLDYLLVLLEAPKPAKQGSPAPPPDPAGTRAEG
                     EPTAPDEFVPAEPEDEEDDSQAVEELQAFADILAARLQALPSVRYVEYKLDTRGPFFD
                     FLRRNQMLFLPPSKLGEVAAKFSDHGIETQLSENVRQLTGPASPLAKRLLEADPLQIS
                     PLLFEAVLRNRGPIRFDLSAGYYSSKDGRSLLVIVKPVKPNQDLAFSKALVAEVNEAI
                     RAAREEFARDVLGAEAPAAAAAAPASGDKETSVAIEARMPEVRLGGGYVIALEDSSLI
                     MQDMVRNGILSFVVIVLLYYFCYRRFAAIAYSAVPLMVGQALTLAVAWIFLRQLNSAT
                     TGFTAMLMGLGTDFTIVMYGRYIEERQKGLPLDAALQKVMGSSAFGVFTGAITSAGTF
                     YAMCITEYKGLKDFGFLVGSGILLCMVAILFLLPAMIAWNDGVRKRKRPVTDKLYLHS
                     FGFERIMIWSTRHPKPVLVGSLLVTLVAGWHAWNVEFSDSVQDLRSPNNQGVRLQERI
                     GKEFGASFNPMMVVAKGRDVETMMARNRAANRLLDGFVEDGTLLGYESIFTYLPPAED
                     QQAVIALLRGHAQDEFDVKRIDSTFRAALGRQGFREGVYDTYLATLPATLRPERPVTI
                     DDLEHAGLDRFVRRYVHQEEDGAWTSVTYIFPSDPKARRVAPPVLVKALDKPKEGIEI
                     TGVNVASAELRRIFKRDAWRAVLLGIALVTVLLWLDFKSVWLTTLANIQVFLGVIWML
                     AAMHLLGIKMNFVNAFVTCMILGVGIDYGIHIIHRITQEGLSNPTGLLETGKAVVMAA
                     LTNVAGFGTIGLSNYPGLKSMGIVCAIGSVTCLLTALTTLPALLIATKTRVPPHEPAA
                     "
     gene            11108..11434
                     /locus_tag="VFD06_15390"
     CDS             11108..11434
                     /locus_tag="VFD06_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04967.1"
                     /translation="MMKPPLLADALPDAAAEVRELAERSGDEGLPEQVPTLRIFDRCR
                     CGEEWCATFYTAPRPSKGWGPKHTTRDLDANAGMIAIDLVDGKIVCVEVLNREDIRGR
                     LLEVIP"
     gene            complement(11453..>11647)
                     /locus_tag="VFD06_15395"
     CDS             complement(11453..>11647)
                     /locus_tag="VFD06_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04968.1"
                     /translation="RDKRGGRRQDSDQTRKGEANALKQELHEPNPRLPNARRQLQAAR
                     NRRMGNHARIEGAPTDSCTP"
BASE COUNT         1831 a         4045 c         3860 g         1911 t
ORIGIN      
        1 gaggtgccgg aggaagagga agccggggaa gagcggcgcg ccgcacatga gctgttcgcc
       61 gttcggcgcg atccagcggc gcgtccgcgg caggtacacc tcgaacccct ccgcggccag
      121 gcgatcgtgc acctgctgct cacattgact gcgcgtcgag agaacgtgcc agttcgcgac
      181 atccgtcatg acgacctcca cctccgaaga aagggacgcg cccactccgg cgcgcgcccg
      241 cgacgtcatt cgagtgcttc gtgattgccg tacgtcaggg tgccggacac ttcgagaacg
      301 atctcccccg cggcgttgcg gacggtcatg accgcgcggt cctccacccc ggacgcgccg
      361 gcgtcggtga aggtccactc ggcggtcgcc tcctcctcgt cgttgtagag gccggagccg
      421 gcgccgctgc acgtgtcgaa gggggcgccg ggcccgtccg gatggatcgc cgggtcgtcg
      481 gcgcaccggc tgctcgagac cgcctcgagg tggaagcgct tcccttccca tgcgatgtcg
      541 aggctgttcg gcagggcgtc cggatcgcag tgcagctcga agccgtgggt gatgacctgg
      601 tcgccgctag cgtggacgct gccgccgccg ctcatccgcg gtccgacggg atcggccgat
      661 gtggtgccgg cggtccggtc gcccgcttcc gcttcgagga cggcgctgag aaggtcgcgc
      721 ttcgttcctc cgagcgtcag gagcgtcatt cccgcgatcc ccgccaaccg ggccatccgc
      781 tgcctcctcc ttccccgcat tgcgctcatc gaaccgccaa tcacttccga tcactttcga
      841 tggcgctggt tttacgacgc gggttgagaa gcggcaatcg ggaggagtgc gtagtccgcg
      901 tgtggcagtt cctgacgtgt accaaagccg tgccggacgt gggtttcacg ggagcgttgt
      961 cggcgtgaaa cgcaccgaaa agtggcctcc ggtgccggca gtcgcggcgc gatgcctccg
     1021 gttcattttc ccgacacggc gcgcagcgac cgccgacttg ccggtgggcc gatccgcttc
     1081 taggctgtgt tcttcaccgg ggacggggag ggaacgagga ggacggatgc tcgctcgagc
     1141 gccggcgcgc gacgccgcgc actatccccc gttgctcgtg gcgggacttt cgtcgcggcg
     1201 catcccgggg ctggacggga tgcgcgccct gggatcgttc ttcgtcgtcg gctaccacta
     1261 cgggctgccg ttcgtgccgg ggggctgggg ggtcctgaat ttcctcgtcc tcagcggctt
     1321 cctgatcacc tggctgctgc tcgccgagga ggatcgcaaa ggatcgatcg ccgttcgatc
     1381 cttctacgtg cggcgcgcgc gccacatcgt gccggcgttc gtcgtcttca tgatcgtcgt
     1441 ggcggcggcg ctgctcgtct tcggcaagcg catcgtctgg ccgcaggtga tcaccgcgtt
     1501 cctgttcgtc gtgaactact accaggggct gttcggcgac ccgaacaccg ccttcagcca
     1561 cacctgggcc ctggcgctcc agctgcagtt ctacctcctg tggccgctgc tgttcctggc
     1621 gctgcggcgc gaccgccggc gcatggccgg cgcgctcctg gtgatcatcg cgtcgatctg
     1681 ggtctaccgc tccctgatgt ggatcggcgg cgtggtgccg caggggtacg tctacgaggc
     1741 gttcgacatg cgcgccgacc acctgctcat cggctgcctg atggcagtgc tcctgcgcac
     1801 cggcagcatg aagccggtgg tcgcggtcgc gaccggatcc ccgtggatgc cgttcctgac
     1861 gatggcgctc atcggcgtgt cggccgcctg cgagaagcac tacggcgtgg actaccgcaa
     1921 cacgctcggg ttcatcgtga acccgatgct gatcatgacg ctcctcctgc aggcgatctc
     1981 gttcgccgcg acgccgatgt ggcgctggct cgagtggcgc tgggtgcggt acctcgggat
     2041 gatttcctat tcggtgtacc tgtaccagca agtggtggtg gacccggtga agcaccgcct
     2101 cgcgttcctg cccgctccac tgcagttcct cgggaccctc gcggcggtgt acgcggtctc
     2161 gagcgtggcg tactacctcg tcgagaaccc gctgcgcggg gcgcgccggc agaggacgca
     2221 gcccaacgtg gtgccgagag tgtgggcagc gaaggtcact cctgagagga tccgaccgtc
     2281 gatgtggagt tcttaaaagg ctctcttcag ggcatgccgg ctattcaccg ggtaaagagg
     2341 agtcgtccag agtcgctggt agatctgcgc ccaggttggc ggagtcggga tccgggccgg
     2401 gaaaagacgt cgagagaaga ctcgcggcca attccagcgg tcgcgcgcca gtccgaggcg
     2461 catcgcgggg gtgccgctgc gggcgactcg ctcgctcctc ttcttgacga aattgcgcca
     2521 cacggccgtc aggaacaggc gttcgagcag ggcattcagg cggcggccga aggcgatcgt
     2581 ctccctccga tgatgcgcca gcgaatgccg cagaatcttg tggagcaggt cgacggggaa
     2641 aagtcgctga tcgcgaagcc gggccacctc gctccgcggc gagcctttcg ggccgcgcgg
     2701 tggattggga tagcgctcga gcctcagacg ccggcctgat ggatgtgtcc gttgggcgcg
     2761 atcataggcg gggtgaccgt ccccgtggat gatcgcctgt gacgcaggcg ggaccaggct
     2821 cagcagtctg tcgaagactc tccgagtcga gccgacatag ccgcctcgtg atggccggcg
     2881 aggacgcgcg ccaagtcgtc ggcgctgtgg ctcactcatg tgaccggtcc ggctgtgcgg
     2941 agccggatcg aggtcgtaca cgtaccagga cttcgaacca acgaccgtcg cgactccgaa
     3001 gggataatcc tgggtaaact cgaaggtctc gaagtggtcc aggacgaacc gctcatcgac
     3061 ttgcccctcg agctcggtgc gcacggcggc gagcagcaag atggcgtgcc gtcccagctt
     3121 cgccgcattc ctggtgaccg tcgaaggcgc acacccgaac gtcctggcga tctgacggtg
     3181 cgcggatccg ccaacaagcc ccgcagcgat cgggacgagc aactccggcc gcttgctgta
     3241 ataggagaca gcgaacgact gccgcgaaaa cgtccctcga catgcacgac aacggaagcg
     3301 cggaatacgc cagcgccgtc ttgtgccgta gaagccgtga cgctcgaacc ggaatccggg
     3361 tcccttgcga acatgctccg gacacccccg cctggggcag aacgagggtt gaaacctcga
     3421 caggtactcg acctgcgtcc ccggccagtg catgccggaa cgtgcaaacc aaagtcgtgc
     3481 cttcgctcgg gccggattcc ttagaaaatc tcagcctgat tctctcgacg cgttcacccg
     3541 cggccgaacc ccgggcgcac gtctccgccc gtgttcacca cactcttagg tccacgttga
     3601 aagctcacgt ggcggcccgg aggaccgccg gcatcacgtc aggcgcggaa cttgatcccg
     3661 cagcccatcg ccttggtctc cttcaccggc acctccttgc cgctcgagac cgcggtgagc
     3721 gcgtccgaga ggtagcggga cgacaccttc tccggatcct gcgagttgtc gtcgatggtg
     3781 ccgtgataga cgagcttccc ctgcgcgtcg aacaggaaca cctcgggggt gcgggtcgcg
     3841 ccgtaagcgc gcgccacgtt cgacgtggag tccacgacgt acgggaaccc gaagctcttc
     3901 tccttggcgc gggtcttcat ctggtcgtag ccgtcctccg ccaccttgcc gggatcgttg
     3961 gagttcacgg cgatgacgcc gatccccttc cccttgtact cgttgccgag tgcgacgatg
     4021 cgatcctccc aggccttggc ataggggcag gcgttgcagg tgaagatgac gagcgtcccc
     4081 ttggccccct tcacgtcggc gaccgcgacc tccttgccgt cgacgttttt catcttctga
     4141 tcggccatcg gaatggccgt tccgatcgcc agcgccttcg gcgcgtcgtc ggcatgaagc
     4201 ggcgcggaga gcgccgcggc gacgaagacc ggagcggcga acgcgagcgc cgcgacagcg
     4261 agcagggaca cgagcgcaaa cgacttcctc atcgaacgat cctccttggg ttcagggacc
     4321 acctggactc agggaccgga gcgggcgctc ccgtccttca ggactttctc gaccatgccg
     4381 cggaacgtcg cgtacgactg cttcccctcg cgcacctcgc gcagggaccc cttgccgtcg
     4441 tagaccagcg tcgccggcag gagcccggac cattcctttg cgagcccgtt cacgaactcg
     4501 tcgtccttcc ccaccttgat gaaggacggg aagtccaccc ccatcgcttc caggaattcg
     4561 accgcgtcgg ccttgccttc gacgaaatcg gccgacacga ggatgacccg gaggccctcc
     4621 gcctcgagat ccttgcggac cttgaggacg tccgggaact cgtcccggca cgggccgcac
     4681 caggtggccc agatgttcac caggaccgcc ttcgcgccgg gggcggtgat cgcctgcttc
     4741 accgccgcgg cgtcggccgg ggtgaggacc ggcttcgccc ccggtttggc ggcagcggcc
     4801 tgcgcggacg gcttcgccgc ggccggcgcg gtcgccgccg gggcggcggc gagaagcagc
     4861 ggcagacaca ggccgagcgg cacgaaactc cggatcctga ggcacatggg agcggagagt
     4921 ctaacccgga cgccccttcc ggggccagcg cgcctcgttc ctcacccacg aggcaagttt
     4981 gggcgctccg cgtcgttgac acctttcaac ccatccggta gcatccgggc cggatcggaa
     5041 tttccgtccc gacgtcgtga cattgcaggg gaagccggcg tgatggccgg ttcgagcccc
     5101 gcgcgatgcg aaccaccgaa cccggtcagg cccggaaggg agcagccgta aggcggacct
     5161 cgcaggtgcc gcgggtccgc tcgggccggc cttcagaccg gctttagaat cgcgcggccc
     5221 ggtcgccgcc gccgcaggcg gtcccgagga acgagacctc ccccgatgag ctaccaggtc
     5281 atcgcccgca agtaccggcc gcagaccttc gaggaggtcg tcggccagcg cccgatcatc
     5341 cagacgctcc agaacgcggt cgagcagcgc cgcctgcacc acgcgtacct cttctccggt
     5401 ccgcgcggct gcggcaagac gacggtggcg cgcctgctcg ccaaggcgct gaactgcgag
     5461 aaggggccga cggcgacgcc gtgcaacgcc tgctcctcct gcgcggagat cgcccagagc
     5521 cggtcgatgg acgtgctgga gatcgacggc gccagcaacc gcggcatcga cgacgtgcgc
     5581 acgctgcgcg agaccgtgcg ttaccacccg gcgcgggacc gctacaaggt cttcatcatc
     5641 gacgaagtgc acatgctgac cgacccggcg tggaacgcgc tcctcaagac gctcgaggag
     5701 ccgccccccc aggtggtctt catcttcgcg acgaccgaat accgcgagat cccgcgcacc
     5761 atcctgtccc gctgccagca cttcgagttc aagaaggtgg cgccgtcggt cctgctcgag
     5821 cacctcaaga aggtggcgca gggggagggg gtgacggtcg agccgtcggc cgcggatctc
     5881 atcgtccggg tggcggaggg gtcgctgcgc gacgcgctgt ccgccctgga ccaggtgatc
     5941 gcgttctccg gcgacaaggt caccgacgag caggcgcgca ccatcctcgg cgtcgtggac
     6001 cgcgacctga ttctcgactt cttcgccgcg gtgcgcggac gggactgcga tcggatcgtg
     6061 gcgatcgtgg acacgctgtt cgagaagggc taccagccgg tggagttcct cgaggacgtg
     6121 ctggcgcaag ggcgtgatct gctgctggcg cgcaccgtct ccgagccatc gaagtacctc
     6181 gccggcacgc cggaggagat ccgggcgctg tccgagcggg ccgccgcctg gagcgaggac
     6241 gagctcctgc gtttcatcga gattgtcacg cgcgaggaaa cgcggatcaa gaactcgagc
     6301 cacgcccggt tcctcctcga ggcgctcggc atccggctgg cgcgcctcgc cgatctcaag
     6361 ccgatcgagg agatcctcgc gtcgctcgag gggcccggct ccggggcgcc gtccccgggc
     6421 gcgaagccgg cgggagcgga tcccggcacc cgcggcgccg cacccggcgc ggcccccggc
     6481 cggccgcccg cggctccggc gcccggacca tcgcggagcg tgtctgccgc gccctctgcg
     6541 gagcccgcgg cgccgatccc ggttgccacc gaggcgccgg tgtcctcgcc cgcattcgtc
     6601 gaggcgctgc tgcagcgggt gcacgaggag cgcgtggcga tcggcagcat gctcgagcag
     6661 gcgatctgga tccaggcggc cgaggacgcg ctgcgcatcg tcttcagcga gaagcacggc
     6721 ttcttccgcg acaaggtgca gagccgcgaa gtgtcggagt acctccggag cgcggtccgc
     6781 tccctgtccg ggcgcgacct gcgcatcgtc gtcgagaccg gcgcccccgg gtcgttcgag
     6841 ccgatgtcgc tcggcgccgc cgcaccgggc atggccgcac cacgcgccgc cgcgagcgtc
     6901 gccacgcccc ttaaccccgc ggccgccgcg ccgcgggcga gcgtgccgac ggcggccccg
     6961 gccggtcccc cggccgcccc gcgcgcgggc gcgacgaccg gcgcggctcc ccgcccggcg
     7021 acgcggatca tcgacgacgc gggacgccag gcgctccgcg atcgtgccct gcaggagccg
     7081 gcggtgcgca gcgtgatgga tctgttcggc ggcgaaatcg tcgacgtcga gccgctctga
     7141 cgcgacgggg cggaccgccc gccccccacg aggagatgcc ccatggacat gcggctgctg
     7201 atgaagcagg cccagaagat gcaggaaaag atgcagaagg agatcgctga gctccaggtc
     7261 gaggggaccg ccggaggcgg catggtcacg atccgaatga ccggcaacca cgccgtcgcc
     7321 tccgtgagca tcaagccgga ggcggtggcg tcgaacgacg ccgagatgct cgaggacctc
     7381 gtgctcgccg cgctcaacga cgcgcaccgc cagatcgagg cggcgctgca ggagaagatg
     7441 ggctccctga agatccccgg gttcggtctc tgagtcgatg cagttcgccg cccccctcga
     7501 gcgcctgatc gagcgcctcc ggaagctccc cggcgtcggt cagaagtcgg cgcagcggct
     7561 cgccttccat ctcctccgcg gcgacgccga ggaggcgcgc gccctggcgc aggcgatcgt
     7621 cgaggtgaaa gagaagatcc gcctctgcac aatttgcttc aacatcaccg acaccgatcc
     7681 gtgcctcatc tgcgccgacc tggaccgcga cgccgcgagc ctgtgcgtcg tcgaagagcc
     7741 gcacaacctg gtgtcgatcg agaagtcgag acagttccac ggccgctacc acatcctgca
     7801 tggctcgatc gcgcccttgc gcggcatcgg cccggacgac ctcaagatcg ggccgctcct
     7861 cgagcgcctg cgcgacggga cggtgaagga ggtcatcctc gccaccaacc cgaacgtcga
     7921 gggggaggcg accgccgtgt acctgtcgcg cctcctcaag ccgatcggcg tgcgcgtcac
     7981 ccgcatcgcc ctcggcctgc cggtcggcag cgagctggaa tacgcggatg aagtgaccgt
     8041 cggcaaggca ctcgaaggcc ggcgcgacat ctagagcacg ggggatccgg gcgagcgatg
     8101 gccggcgtca agaaggggtt gttcctccgc atcgaggact tcgcccgccg gcgctacgcc
     8161 gtcgtcttcg cggtcaccgc gctcctcgtc ctcgcctcga ttctcctcgg caagcggctg
     8221 cggctcgacg gcgacatcct caacctggtt ccgaagggga accgggtggt caacaccttc
     8281 cgcgaggcgc tccaggactt cggcagcctg gactatctgc tggtgctgct cgaagccccg
     8341 aagccggcga agcagggctc ccccgcgccg ccacccgatc ccgcgggaac gcgcgctgaa
     8401 ggcgagccga ccgcccccga cgagttcgtc cccgccgagc cggaagacga agaggacgac
     8461 agccaggcgg tcgaggagct gcaggcgttc gccgacatcc tggcggcgcg gctgcaggcc
     8521 ctcccgagcg tccgctacgt cgagtacaag ctcgacacgc gcggcccgtt cttcgacttt
     8581 ctccgccgca accagatgct cttcctgccg ccgtcgaagc tcggggaggt ggcggcgaag
     8641 ttcagcgacc acggcatcga gacgcagctc tccgaaaacg tccggcagct caccggcccc
     8701 gcctcaccgc tcgccaagag gctcctcgag gccgacccgc tgcagatcag cccgctcctg
     8761 ttcgaggcgg tgctgcggaa ccggggcccg atccgcttcg acctgagcgc cggctactac
     8821 tcgtcgaagg acggccgatc gcttctcgtc atcgtcaagc cggtgaagcc gaaccaggac
     8881 ctcgccttct cgaaggcgct cgtggcggag gtcaacgagg cgatccgcgc cgcgcgcgag
     8941 gagttcgcgc gcgacgtcct cggcgccgag gcccccgcgg cggccgccgc ggcgccggcc
     9001 tccggcgaca aggaaacctc cgtggcgatc gaggcgcgca tgccggaggt gcggctcggc
     9061 ggcggctacg tcatcgccct cgaggacagc tcgctcatca tgcaggacat ggtgcggaac
     9121 ggcatcctgt cgttcgtggt gatcgtcctt ctttactact tctgttaccg ccgtttcgcc
     9181 gccatcgcct actccgcggt gccgctcatg gtcggacagg cgctgaccct cgccgtcgcc
     9241 tggatcttcc tgcggcagct gaactccgcg acgaccgggt tcaccgccat gctgatgggg
     9301 ctcgggaccg acttcaccat cgtcatgtac ggccgctaca tcgaggagcg gcagaaaggg
     9361 ctgccgctcg acgcggcgct tcagaaggtc atgggctcgt cggcgttcgg agtcttcacc
     9421 ggcgccatca cctcggccgg caccttctac gcgatgtgca tcaccgagta caaagggctg
     9481 aaggatttcg gcttcctggt cggctccggg atcctgctgt gcatggtggc gatcctgttc
     9541 ctgctgccgg cgatgatcgc ctggaacgac ggcgtgcgga aacgcaagcg ccccgtcacc
     9601 gacaagctct acctgcactc gttcggcttc gagcggatca tgatctggag cacccgccac
     9661 ccgaagccgg tgctcgtcgg ctcgctgctc gtgaccctcg tcgccggctg gcacgcgtgg
     9721 aatgtcgaat tcagcgacag cgtccaggac ctgcgctccc ccaacaatca gggcgtgcgc
     9781 ctccaggaga ggatcgggaa ggagttcggc gcctcgttca acccgatgat ggtcgtggcg
     9841 aaggggcgcg acgtcgagac gatgatggcg cgcaaccgcg ccgccaaccg cctcctcgac
     9901 ggcttcgtcg aggatgggac gctcctcggc tacgagtcga tcttcaccta cctgcccccg
     9961 gccgaggacc agcaggcggt catcgccctg ctgcgcggac acgcgcagga cgagttcgac
    10021 gtgaagcgca tcgacagcac cttccgcgcc gccctcggcc ggcaggggtt ccgcgaaggg
    10081 gtctacgaca cctacctcgc gacgctgccg gcgacgctcc gtccggagcg cccggtcacc
    10141 atcgacgacc tcgagcacgc cggcctcgac cgcttcgtcc gccgctacgt gcaccaggag
    10201 gaggacggcg cctggacctc ggtgacctac atcttcccgt ccgacccgaa ggcgcgccgc
    10261 gtcgccccgc ccgtcctcgt gaaggcgctg gacaagccga aggaagggat cgaaatcacc
    10321 ggcgtcaacg tcgccagcgc cgagctgcgc cgcatcttca aacgggacgc ctggcgcgcc
    10381 gtgctcctcg gcatcgccct cgtcacggtg ctgctctggc tggacttcaa gagcgtctgg
    10441 ctgacgaccc tcgccaacat ccaggtcttc ctgggcgtga tctggatgct cgccgccatg
    10501 cacctgctcg gcatcaagat gaacttcgtc aacgcgttcg tcacctgcat gatcctcggc
    10561 gtcggcatcg actacggcat ccacatcatc caccgcatca cgcaggaagg cctgagcaac
    10621 cccaccggcc tgctggagac cggcaaggcg gtcgtgatgg ccgcgctgac gaacgtcgcc
    10681 ggcttcggca ccatcggcct gtccaactac ccgggcctga agtcgatggg gatcgtctgc
    10741 gccatcgggt cagtcacctg cctgctcacc gcgctgacga cgctgcctgc gctgctgatc
    10801 gcgacgaaga cgcgtgtgcc cccgcacgaa ccggcggcct gagccgtttt cttcgccaca
    10861 tttccccgct gcgttttatt gatttaggct gaccaaccct cgcagcctat tcccctaaac
    10921 acccaccacc ccgaaggtgc taacctcttt ccagtcaagc gactcggcca gcccgccgcg
    10981 ctcaccgccc gatttagggc tgaccgaccc gcgctccacg agttttttga cgagggctgt
    11041 ctacttagcg ttgggcagac atagtgttcc agcgggccga agtggcatta gacgcgagtg
    11101 caggtagatg atgaagccac cgttgttagc cgacgcgttg cccgatgccg cagccgaggt
    11161 gcgcgagctt gcggagcgaa gtggggacga ggggttgcct gaacaagttc ccacgcttcg
    11221 gatcttcgac cggtgtcgtt gtggcgagga atggtgtgcc acgttctata cagcgcctcg
    11281 tccttccaag ggctggggcc ccaagcacac cactcgtgat ctcgatgcca acgccggcat
    11341 gattgccatc gacttggttg acggcaagat cgtgtgtgtc gaggtgctga accgtgaaga
    11401 catccgtggg cggttgcttg aagtgatccc gtaatgggtg cgtgctgccc aactacggcg
    11461 tgcagctgtc agtcggcgcg ccttcgatcc gggcatggtt gcccatccgt cggttgcgcg
    11521 ccgcctgcag ctgacgccgg gcgttgggca gacgagggtt aggctcatgg agttcttgct
    11581 tgagagcatt cgcctcccct ttacgcgtct gatcgctatc ttgacggcgg ccgccacgct
    11641 tgtcccg
//
LOCUS       DASRIQ010000621         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1023858, whole genome shotgun sequence.
ACCESSION   DASRIQ010000621 DASRIQ010000000
VERSION     DASRIQ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023858"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3312)
                     /locus_tag="VFD06_15400"
     CDS             complement(<1..3312)
                     /locus_tag="VFD06_15400"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HZN04969.1"
                     /translation="MPEIRRCLVWGEGPEKPACLGAATPVVPAPSPDPSGARSPARVL
                     LLLGILALPAIGAARAGDFDVPGAGPLPSFGVPSADPATGLATAVVPFEVPPGRHGLA
                     PALALRYSSAGGAANAGLGFSLSIGSIERSTRTGPPRFDNTDTFVLILDGRAWDLVPI
                     DAASTRFRTVLQSGFLVERVRPGPWGAGSTSFVARDREGRRYRFGAVAGAASTSQVAD
                     FKWGLDRVEDESGNVMDIDWAAVSRHLYPLRIAYASHPATGLEPSNVVELCWEERGDR
                     PLSPSGEAFTHRLREVRTFAGALPARRFTLTYASIGDTANTLGTCRSSLDLSGPVPTG
                     SDPADTPTPGRPGGARRIEPSDSEGPTSGTTTTLSLSRLAPTATATPVSMPVTRTGSS
                     SAPGDPTIEVVAPAPALPPVPSLLVRIERADGSGSLLPPIDYTYASGGLPSWPAAGPP
                     SLAPPVPFLYAKPDADEDAGARLVDLNRDGLPDVVQMEGRLSGLSASTVAAAWLNTGQ
                     SFQYSAAWSTALLALVHPTDRSRSAWFVIKRDARDRVENGVRFADVNDDGRIDVVRLV
                     LWFGQGIRKQVFLNTGVGFTSDVAGSFAIPDEPFVDLQSESTQDTAADRGVRLADLDA
                     DGRVDLLVARAEWGAAAERRVWRHNGAGWSLDARFILPDEPFVRHIPHGRCLDTGLRL
                     VELNGDGFPDLYRAAAVDGIVSSATYVHTGTPDAVHPTWEKTTQWGFLGTQAEHFVQI
                     STAGDGAVLDRGLRVADVNGDGKSDIVVGRRWNGGPAETFLFSPAPTGQWSWRPFPEL
                     PWIFVDRRVDEPSRDQGVRLGDLDGDGGLDLLLVADTVGRAWRPNLSWRGRAALASCT
                     NGLGGRVDLVYAPAPHGGTIEGGGAAALPFAYPVVAEIRAWDGIGQTLVTRFRYSGGL
                     YHHATRDLRGFRQVTATPPGGEMEIESLLVQQPTLAAAPLRGAVAERLERRTADGAIL
                     SRTIRTFDESDLLPPMRHPILREETRFFDWGTTDPASGAWSRRVATSWTWVFDGSAGT
                     ESPLLRSDERREGDTSDPTDDRTLRTEYASLVSAETTGAAGAGAWSLEQPWHETTLGA
                     DGNVVAESWTAWDGAP"
BASE COUNT          500 a         1231 c         1302 g          430 t
ORIGIN      
        1 cggcgcgccg tcccaggcgg tccaggactc cgccacgacg ttcccgtccg cgccgagcgt
       61 cgtctcgtgc cacggctgct cgaggctcca tgcccccgct ccggcggcgc cggtcgtctc
      121 cgcgctcacc aggctcgcgt attccgtccg gagggtccgg tcgtccgtcg ggtcggaggt
      181 gtccccttcg cgccgctcgt cgctccgcag gagcggactc tcggtcccgg ccgacccgtc
      241 gaacacccac gtccacgagg tcgccacccg gcggctccag gcgccgctcg cgggatcggt
      301 cgtgccccag tcgaagaagc gcgtctcctc ccggaggatc gggtgccgca tcggcggcag
      361 gagatcggat tcgtcgaagg tgcggatcgt gcgagagagg atcgcgccgt cagcggtacg
      421 ccgttccagc cgctcggcga ccgccccgcg cagcggggcc gccgcgaggg tgggctgctg
      481 caccaggagc gattcgatct ccatctctcc gccgggcggc gtcgccgtca cctggcggaa
      541 gccgcgcaga tcgcgggtcg cgtggtggta gaggcccccc gagtaccgga agcgggtcac
      601 gagcgtctgg ccgatcccgt cccaggcgcg gatctcggcg acgaccgggt acgcgaacgg
      661 cagcgccgcc gccccgccac cttcgatcgt cccgccgtgt ggcgccggcg cgtagacgag
      721 atcgacgcgc cctcccagtc cgttggtgca ggaggcgagc gccgcccgcc cccgccacga
      781 gaggttcggc ctccaggcgc gtcccacggt gtcggcgacg agcaggagat cgaggccgcc
      841 gtcgccgtcg agatcgccga gccgcacccc ctgatcacgg ctcggctcgt cgacccgccg
      901 atcgacgaag atccacggaa gctccggaaa cggccgccac gaccactggc cggtcggggc
      961 gggggagaag aggaaggtct ccgccgggcc gccgttccac cgacggccga cgacgatgtc
     1021 gctcttgccg tcgccgttga cgtcggcgac gcgcagcccg cgatcgagca cggcgccgtc
     1081 gccggcggtg gagatctgca cgaagtgctc cgcctgcgtt cccagaaaac cccactgggt
     1141 cgttttctcc caggtggggt ggacggcgtc cggggtcccg gtgtggacgt acgtcgcgct
     1201 gctcacgatc ccgtccaccg ccgcggcgcg gtaaaggtcg gggaacccgt cgccgttcag
     1261 ctccacgagc ctgaggccgg tgtcgaggca gcggccgtgc gggatatggc gcacgaacgg
     1321 ctcgtccggc aggatgaagc gcgcgtcgag gctccagccg gcgccgttgt ggcgccagac
     1381 gcggcgctcc gcggcggccc cccactcggc gcgcgccacc aggaggtcga cgcggccgtc
     1441 ggcgtcgagg tcggcgaggc gcacgcctcg gtcggccgcg gtgtcctgcg tggactccga
     1501 ctgcagatcg acgaacggct cgtccgggat ggcgaaggag ccggcgacgt cgctcgtgaa
     1561 cccgaccccg gtgttgagga agacctgctt gcggatcccc tggccgaacc acaggacgag
     1621 tcgcacgacg tcgatccggc cgtcatcgtt gacgtcggcg aaccggacgc cgttctcgac
     1681 ccgatcgcgc gcgtcgcgct tgatcacgaa ccaggcgctc cggctccggt cggtcgggtg
     1741 cacgagcgcc aggagcgccg tcgaccaggc ggcgctgtac tggaaggatt ggccggtgtt
     1801 gagccaggcc gcggcgacgg tggacgcgga aagaccggag agacgaccct ccatctggac
     1861 gacgtccggc agtccgtcgc gattcagatc cacgaggcgc gcccccgcgt cctcgtcggc
     1921 atccggcttc gcatagagga acgggaccgg cggtgcgagg gagggtgggc cggcggccgg
     1981 ccaggaggga aggccgcccg aggcgtaggt gtagtcgatc ggcggcagga gcgatccgga
     2041 tccgtccgcg cgctcgatcc gcacgaggag cgacggcacc ggtggcagcg cgggagccgg
     2101 ggcgacgacc tcgatcgtcg gatcccccgg cgcgctcgag gacccggtgc gggtcaccgg
     2161 catcgacacc ggcgtcgccg tcgcggtcgg agcgagccga ctcaatgaga gcgtcgtggt
     2221 cgtgccggag gtcggccctt cggaatcgga cggctcgatg cgccgcgcac cccccgggcg
     2281 gccgggggtc ggagtgtcgg ctggatcgga accggtcggc accggcccgg agagatccag
     2341 gctcgagcga caggtcccga gggtgttcgc ggtgtcgccg atcgaggcat aggtgagcgt
     2401 gaaacgacgc gccgggagcg cgccggcgaa ggtccgcacc tcgcgcagcc ggtgggtgaa
     2461 ggcctcgccg gacggtgaga gcggccggtc gccgcgctcc tcccagcaca gctcgacgac
     2521 gttggacggc tcgagcccgg tcgcggggtg cgacgcgtac gcgatgcgca gcggatacag
     2581 gtgccgcgac accgccgccc agtcgatgtc catgacattg ccgctctcgt cctcgacccg
     2641 atcgagtccc cacttgaagt cggccacctg cgacgtggac gcggctccgg cgacggcgcc
     2701 gaagcgatag cgccgccctt cgcggtcgcg cgcgacgaag gacgtcgatc cggcccccca
     2761 cggtccgggg cgcacccgct cgacgaggaa cccgctctgg aggacggtgc ggaaccgcgt
     2821 cgaggccgcg tcgatcggga cgaggtccca ggcgcggccg tcgaggatca gcacgaacgt
     2881 gtcggtgttg tcgaagcgcg gtgggcccgt gcgcgtggac cgctcgatcg agccgatcga
     2941 gagggagaag ccgagaccgg cgttcgccgc cccgccggcg ctcgagtagc gcagggccag
     3001 cgccggcgcc aggccgtgac gtccgggcgg gacctcgaac ggcacgaccg ccgtcgccag
     3061 cccggtcgcc ggatcggccg acgggacgcc gaacgacggc agcggtccgg caccggggac
     3121 atcgaaatca ccggcacggg ccgcgccgat cgcgggcagc gcgaggatcc cgaggagaag
     3181 gaggacgcgc gcaggcgacc gcgcgccgga agggtcgggg gaaggggccg ggaccaccgg
     3241 agtggccgcc cccaggcacg ccggcttctc cggcccctcc ccccacacga gacaacgtcg
     3301 aatctccggc atcgattcct ccagcggcgc gcctggtgtc gcggcggact cgggagggca
     3361 aggccggtgc cacagctggt gccgggattc aggggtgcga ggcgatcgcg cgcaaatgcg
     3421 tgcagaacac gggcttacgc ctcagcgggg gacgctacgg cgg
//
LOCUS       DASRIQ010000622        16555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1561887, whole genome shotgun sequence.
ACCESSION   DASRIQ010000622 DASRIQ010000000
VERSION     DASRIQ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561887"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="VFD06_15405"
     CDS             <1..1973
                     /locus_tag="VFD06_15405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN04970.1"
                     /translation="LALLALGSGASTPSARPLRLEETVDFAGIEDLRLSPDGRTAALC
                     VRKRGAGENRFATDIYVVPADGSAPERALTRAPGNDCQPRFSPDGRRLAFVSDRSGAD
                     QVWVMPVDGGEPVQATRGEGIGTFAWSPDGKAILFTASELPAESERRRRERGDDARLY
                     GDWPLSSLWRAPLSQTNGEGAPGTPVRLTDGATHVTDLIAPSPDGTRVAFVSQPTPEA
                     DASEEATVKVMEIATRMVREVPGSRRASALAWPGTAPPPGRGGPPSGALLFARPFDGA
                     GWSRADLFAWRTDETGPRNLSAAIDRDIEQILVTADGGAEVLWSRGAVTQIAPVGADG
                     TLGRPWSPLYPVGSSARRGEERLFVRLDRPHEVWRADRSGGVRPITRLNAALTGTVDL
                     PTLETFRWKSGAWEVEGVLTVPPGAAAAGPRGGPYPLLIRPHGGPRANSLLEFSPQDA
                     WLASLGYLVLEPNFRGSTGYGDRFAKGNGGDWGAGPFADIMAGVDALIAAGRADPKRL
                     YLFGWSYGGIMANWAATHTDRFRAIVSGAGVADLRMQYILSDARRWRFDYFGGSPFEP
                     QHLPVYWANSPVTQVGKVTTPVLFIQGEEDRRCPLPQALMMHRAILDNGGDSTLVLYP
                     REGHGFREPEHILDRTRRIAAFFARHGGPDSP"
     gene            2069..3451
                     /locus_tag="VFD06_15410"
     CDS             2069..3451
                     /locus_tag="VFD06_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04971.1"
                     /translation="MLLFLGLWLLYGAGINKLNLQGYTLHPEVVMAIAEEGTFVIGHQ
                     EVPKENDRFLFDGNVLPAKQPWIFALGAAAYLPVRALGLTYDRDFFLAEAVITWLTAS
                     LFAAVAAAAALVVARRVWGFRRRDALLAVLAGAACTILLPYAGIPHHDVVATALLVLA
                     LAAFEVARSDEAAGTSGKARRRLMASGLAGLFLGLMLGFSMLPAAIALGVIVAIAATR
                     NRSIVVPAAGGFVLGLLPLALYNGHYFHTPFLQANLAGQYDDTYPGFAPERIVHHLNA
                     YLGVGEVSILKYMPILLAGAAGLVLLAVRGRAPGRLLLTLLLLHGMYLLAIPAIGYCQ
                     YGPRFLLPAVPLAAFGLAPLLEALDRLQGGWRLAGAALVALLALASLMVNLVGTVGGT
                     MYCAIEEFAFPRYVAVLADPQAWTAIATQFPLLGFCSAGLLLLGVAGFGGVLLARGRA
                     ASLDASVTGR"
     gene            3464..6007
                     /gene="rnr"
                     /locus_tag="VFD06_15415"
     CDS             3464..6007
                     /gene="rnr"
                     /locus_tag="VFD06_15415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZN04972.1"
                     /translation="MNDARHLRDAILDFMRRPNYRPVSADELQRLLRIDRSRRHPFSR
                     ALRALVNDDAVIRVDRHRYAIAGWTPTVPRSGGRDGRGDGGRRVERRDGGRHGRTAEP
                     RVRDVPAQGAAAPAAGPMRAGSIVTGNIQVHPKGFAFVTPDRGGVDLFIPPPAVGDTQ
                     DGDRVEAIVVRMQADGRATGDIRRVLEKTRRRIVGVYRGGPDGGTVEAYDRRFEAGIA
                     IPREGVGGAVNGLVVGVEVEQQAEEGRSAWGRIVEVIGPAESPETDLKAVIRKFGLRQ
                     EFPPDVMQQAAQAPDRVPPEDIRGREDFRDLPIVTIDGETAMDFDDAVLVRRLDGGHY
                     ELHVHIADVAHYVRGSSPLDREALERATSVYFPGTSLPMLPHRLSNGICSLNPHVDRL
                     VLSCVMTIDPQGQVTQYRLAEGVIRSAARMTYTNVAKILVDRDPRVMTQYRDFVAGFR
                     LMEELCGVLNARRRRRGSIDFDLPEPEVILAATGEMTGIMALERNIAHRLIEEFMLTA
                     NETVARHLFRAKVPSMYRVHERPDPRRLEDFEAVAHAFGYSLPRPFTSIEPRSFQSLL
                     ERARGRPEERFLSRLMLRSMKQARYSEKRDIHFGLASTCYTHFTSPIRRYPDLVVHRL
                     VKRLLKGGPVQARERGDLEVFMPEAAARSSVRERTADAAENELIERKKMVFMAQKIGE
                     EFDGFICGVEAFGIFVELQELFVDGLVGIDALPGDRFRFIERQRIMKGERHGQVLALG
                     DPVRVRLSRVSAARMEIEFALLEHRPGPRPAGVAAAVREPAETRAGRRDVARGKRRQD
                     ERKRAAASGAAAPGRGHAPARHGRRGRTRGGGGSTGAGSVRSGRGRRRR"
     gene            6013..6495
                     /locus_tag="VFD06_15420"
     CDS             6013..6495
                     /locus_tag="VFD06_15420"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HZN04973.1"
                     /translation="MARRRRAAAPRRGVPAARRRAAPYRFLPHTADMAVAVRERTLAR
                     LFENSAAALFDLVTDRGVVRPQRSVRFGARGQSLDDLLVRFLSELLYLQEVKGWRFRC
                     CAVEKLDRAGLRVRGAAEGEPFDSERHPRRHEVKAITYHRLGITRSAGQWQVTFVVDV
                     "
     gene            6593..7414
                     /locus_tag="VFD06_15425"
     CDS             6593..7414
                     /locus_tag="VFD06_15425"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN04974.1"
                     /translation="MEWFYVADQRQVGPLSEAEFARLVGAGTILGETLVWRVGMKEWQ
                     PYSALPPIAPPTTPAAGDPGADWPVTQGSPVAAASSAMGTCTQCGRSLPATEMLAYAG
                     GYVCPTCKPMFVQRLKEGARVTAGVRYGGFWIRFAARFVDGIILWIANVMVLLPFGLG
                     AAGTMLRGTPPEAADLPSLLAMQGLLILIQVGIAASYEIFLTTKYGATLGKMILRLKV
                     VRPDGGSIGFGRAVGRYFSTWISGFTLGIGYIIAAFDDEKRSLHDRICDTRVVRA"
     gene            7411..8013
                     /locus_tag="VFD06_15430"
     CDS             7411..8013
                     /locus_tag="VFD06_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04975.1"
                     /translation="MSPACPGCRTALPPDILGAEGETSCPRCHIPVEVEVFPALTRPL
                     EAASGSAVQSAEGESTCFYHAGYRAAVPCDACGRFLCSLCDLEIGSRHLCPECIHRSI
                     QGPKATMTSERILWDRLALSLAVLPIFTFYFTVVTAPAAIFIALRHWRSPGSLVSHGR
                     WRFVVAVLCATLQLAGWAGIIYAIARAGGTAVETAVDTED"
     gene            8018..9019
                     /locus_tag="VFD06_15435"
     CDS             8018..9019
                     /locus_tag="VFD06_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04976.1"
                     /translation="MTPPAPPPLYRRLPGRRRGLATSATLWQADDHLLVAEVRFWTES
                     YRRIYYRDIQAIVLGQDGRWLALNLMFLTFGTLLLLVVAGTQGGWRIFWAFPAALVVL
                     LGSVHFLRGPTCACEVTTPLATLRLPLQRVRPARRTLALVRSRIEAAQGTATREKIVA
                     QAAEGPPAAPPAAPILLSRTPPPLVRPGQPRMHAVLCAALVLDAMVTVYQWPHGNTVI
                     DSVAVVTFFGCVLASIFAIVAQNRTDLGPEFRWFGVTALGAQILLFVAGWIFLFAGMI
                     KQAGAGGAPQFPALFASGTEAIGPYEIAMQSILALWGLVVLLRWRRARKTAWISLTT"
     gene            8998..9327
                     /locus_tag="VFD06_15440"
     CDS             8998..9327
                     /locus_tag="VFD06_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family protein"
                     /protein_id="HZN04977.1"
                     /translation="MDQPDDLRLRRCLHHATREAVARCPRCGRSFCRECISEHDGLWL
                     CAACLAAEGEGSAARRGRLVLVWSGLRVGAGLFVAWFYFYLVGQALLAFHDPIHPGGD
                     RPGVTAP"
     gene            9324..10955
                     /locus_tag="VFD06_15445"
     CDS             9324..10955
                     /locus_tag="VFD06_15445"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HZN04978.1"
                     /translation="MRRSGGGIDILDDAVRLLRAAPWSAHLCFHAGSLPFVFGLLFFV
                     VDMRRSALAPEHAGSAALLMALLYLWMKGWHAAAAVRLRRALAGGDAERERGMARRVL
                     AQCAIQPWSLFLLPAALVLTLPFAWVYAYYHAATLTSDAAAAREQSAFRPMENHLLVL
                     ILSAGAFVVWTNLLVIVLALPFLLSTFFGIETPFSRTPMLLFDSTVALLVSGLAYLVM
                     GPVALAAYLVRAERAESARTGRDLLRGIDALPPPVTGGRAVACLLAGLLGAACAAASA
                     EAAVPVITEAPATVAKPAGGIPVAELDDALRRATARPEYLWRMPRPAPPPDEDEPGLF
                     GRFGRTIASTLRSWWDGLSRAVQRLARWIDRHFETGEPAEHRVGMPGWPAGASWALLG
                     VIVLLVVSAGVSWWRRRTKSAEVRATVVAPRGIPVDLSSAAPTDAPSDEWLERSRGLL
                     ERGELRFAARAVHLATLALLGEARLLTLGRARSDRDYLVELRRRRPDRPEIADLFLDG
                     VRDFERVWYGRHPASVDLVRGLGERCERLRHYAQG"
     gene            10942..12132
                     /locus_tag="VFD06_15450"
     CDS             10942..12132
                     /locus_tag="VFD06_15450"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HZN04979.1"
                     /translation="MRRDRLALAALLGLAVLFAAGLALVARERYARGDGYPAGSSFRT
                     DPLGTKALHDALLEIEGLRVARHVGPATDLRGGEGTVVLLLEVSDRLGTADPVLVRAV
                     EAVGLAGGRIVVTLARSQRSWFEDEDDEDPKESEKKREDERAEQGEREPQASFAPPLS
                     ARWGFAARPCPSEPHATIAAVPIATEESSGDLPWRGGDCLADLTPAWRSLYRLGAHPV
                     VAERSLGRGSIVLLTGGYVLSNEGLLFDRRPAVLSRLIGTDARTILFEESHHGVRRRA
                     GIVALLGRYRMRGALAAAAILLGLWGWWAGSPLVPAAGGAETEPRSGKEASLGLVSLL
                     RRGVPPKRLMETCLEEWRRAFARARPAAAAALARLPPPGEDIPAAYRRAREAVNPNGG
                     DDDR"
     gene            12122..13087
                     /locus_tag="VFD06_15455"
     CDS             12122..13087
                     /locus_tag="VFD06_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN04980.1"
                     /translation="MIAETERLHEVLTRAREEVARVIVGQADAVDQALAVILTGHHAL
                     IEGMPGVAKTLLVRSLAAVLGCEFARIQFTPDLMPSDITGTNVFNLKTSEFVFHPGPV
                     FTAFLLADEINRAPAKTQSALLQAMQERAVTIDRQTRPLPPQFTVFATQNPIEFEGTY
                     PLPEAQLDRFMLKIRMDPPAADEELALARRLLGDEAPEAVLAAGGIRPVVGREDLVRL
                     QRALMAVTVRDEILGYAVEIVRRTRAHDAILVGASPRGLAALLHAARAQAAIAGRDFV
                     TPDDVRRIAVAVLGHRLVLRPEYEIEGEEIGAVVARVLAEVPVPR"
     gene            13084..14406
                     /locus_tag="VFD06_15460"
     CDS             13084..14406
                     /locus_tag="VFD06_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN04981.1"
                     /translation="MIAPRPRLLLAVGGIALPLAALLPALPERSGEAVLLALAAIVAA
                     DALLGPRRLAGIEVLLPSTARFLRDREGEIAIRVRHLNPTSRLVAVGLSLPPALASRS
                     DPLRVRLPAGPSEAVFAWPLRPRSRGRFRITALHLEASSPLGFWGARRAVEVATEVRV
                     YPNLLAEGRGAAALFLRRSRVGLHALRRIGKGREFESLRDYQPGDGFEDVHWKGTAKR
                     GRPVTKVFQVERTQEVYAIVDASRLAARLQGDPPSAAIERFVSAALLLARATERQGDL
                     FGLVAAADSVSMFLRAGRGRAHFDACREALCALEARPATPDFDETATFLLSRLRRRAL
                     LLFLTDLDDPVLAEGFLRCAGVLARRHLVVACLLRPHGARPLFTGPDPADPDDVYRRL
                     AGHLVWSDLQELTRGAQRKGAAVSLFEGSSLPTDVVGRYLEVKRRQAL"
     gene            14403..15392
                     /locus_tag="VFD06_15465"
     CDS             14403..15392
                     /locus_tag="VFD06_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZN04982.1"
                     /translation="MILDLPRFLASERPFWSELEEWLDRLETDPHRTMSLEEARRLHY
                     LHQRASADLARIQSSAAEPQTRAWLEALVGRAYGEVHETRDRTHRLRPLRLFLHDFPR
                     AFRRRAGAFALACGMLLAGTALGSIALLVDDGAREVVLPAELASLSPSERVHDEETRR
                     TDRLQGQRSRFSAMLMTHNTRIALFCLALGVTYGIGTAILLFHNGVVLGAVAADYVAD
                     GQTRFLLGWLLPHGIVELPAIAIAGQAGLVLASALLGRGDGTPLRERLAVAGRDVGAL
                     AAGLATLLLWAGLMESFLSQHHEPVLPYALKIGIGAVEGVLLVLFLSLAGRRP"
     gene            15389..16165
                     /locus_tag="VFD06_15470"
     CDS             15389..16165
                     /locus_tag="VFD06_15470"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN04983.1"
                     /translation="MSRDAVTIRTPEGIVFALPLAGPGARFVAWGVDLACVGAVGSLL
                     ATAIRGLLGFQPDIAEAVGAVLYFGLSILYGLVLEWVWRGQTLGKRLVGLRVMDEEGR
                     RLQPSQVVVRNLLRSIDVLPIFYLVGGAAMLASRHWQRLGDLVACTIVVRAARTDPPD
                     PDALRSDKYNSLRGRPRIEARLRQRISPAEAALAVQAIVRRDAIEPAARVAIFRRLAQ
                     RFVAAAEIEPALFESVPDEQVVRNVLEIVLTRPGRAGVST"
     gene            16226..>16555
                     /locus_tag="VFD06_15475"
     CDS             16226..>16555
                     /locus_tag="VFD06_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HZN04984.1"
                     /translation="MEWFYVENQQRVGPVSEGEFARLVGAGTIRRETLVWRAGMKDWQ
                     PYGSLLPPPPPAPPAASGGPIPSTPVAGSPQAGSPGVPSPHSSAPGGAAATARGGTFS
                     AASATMSP"
BASE COUNT         2016 a         5944 c         6053 g         2542 t
ORIGIN      
        1 ctctggccct tctcgctctg gggagcggcg cttcgacccc ctcggcccgg ccgctgcggc
       61 tcgaagagac ggtcgacttc gccggcatcg aagacctgcg cctttcgccg gacggacgga
      121 ccgcggccct ctgcgtccgg aagcgcgggg cgggggagaa ccggttcgcc accgacatct
      181 acgtcgtgcc ggcggacggg tcggccccgg agcgggcgct gacacgggcg ccggggaacg
      241 actgccagcc gcgcttcagc ccggacggac ggaggctggc gttcgtgtcg gatcgatccg
      301 gcgccgatca ggtctgggtc atgccggtgg acggcggcga gccggtgcag gcgacgcgcg
      361 gcgaagggat cggcaccttc gcctggagtc cggacgggaa ggccattctg ttcacggcgt
      421 ccgagctgcc cgccgagagc gagcggcggc gccgcgagcg cggcgacgac gcccgactct
      481 acggcgactg gccgctgagc agcctgtggc gcgcgccgct gtcgcagacg aacggggagg
      541 gtgcgccggg aacgccggtt cgcctcaccg acggcgcgac gcatgtcacc gatctcatcg
      601 cgccgtcccc cgacgggacg cgggtcgcct tcgtgtccca gccgacgccc gaggcggacg
      661 cttcggaaga ggcgaccgtg aaggtgatgg agatcgccac ccgtatggtg cgtgaggtcc
      721 ccggatcgcg gcgcgcgtcg gcgctggcgt ggccggggac cgcgccgccg ccgggacggg
      781 gcgggccacc gtccggtgcg ctgctcttcg cccggccgtt cgacggggcc ggctggtcgc
      841 gcgccgatct ctttgcgtgg aggaccgacg agaccggacc gcgcaatctc tcggcggcga
      901 tcgatcgcga catcgagcag atcctcgtga ccgccgacgg gggggcggag gtcctgtgga
      961 gccgcggggc ggtcacgcag atcgctcctg tcggtgccga cgggacgctc ggccgtccct
     1021 ggtcgccgct ctacccggtg ggatcctcgg cgcgccgcgg cgaggagcgc ctcttcgtcc
     1081 gtctcgatcg gccgcacgaa gtctggcgcg ccgatcgatc cggcggcgtc cggccgatca
     1141 cccggctcaa tgccgcgctc accgggacgg tcgacctccc gacgctcgag acgtttcgct
     1201 ggaagagcgg cgcgtgggag gtcgaagggg tcctgaccgt gcctcctggc gccgctgcgg
     1261 ccggcccgcg gggcggcccg tatcctttat taatacggcc gcacggcggg ccgcgcgcca
     1321 acagcctcct cgagttctcc ccccaggacg cgtggctggc gagcctcggg tatctcgtcc
     1381 tcgagccgaa cttccgcggc agcaccggct acggggatcg cttcgccaag gggaacggcg
     1441 gcgactgggg ggccgggccg ttcgccgaca tcatggccgg ggtggacgcg ctgatcgcgg
     1501 cggggcgggc cgacccaaag cgcctgtacc ttttcggctg gtcttacggc gggatcatgg
     1561 cgaactgggc ggcgacgcac accgaccggt tccgcgccat cgtcagcggc gcgggcgtcg
     1621 ccgacctgcg catgcagtac atcctgagcg acgcgcgccg ctggcgcttc gactatttcg
     1681 gaggatcccc gttcgagccg cagcacctgc cggtctactg ggccaactcg ccggtgacgc
     1741 aggtcggcaa ggtgacgact ccggtcctgt tcatccaggg agaggaggac cggcggtgcc
     1801 ccctgccgca ggcgctgatg atgcatcgcg ccatcctcga caacggcggc gacagcacgc
     1861 tcgtgctcta tccgcgcgag gggcacggct tccgcgagcc ggagcacatc ctcgatcgga
     1921 cgcgccgcat cgccgcgttc ttcgcccggc atggcgggcc cgactcccca tgaagtcacg
     1981 ccgctccgga gcggcggtcc ccgcgccccg acagtcgcct gccgtttcca ccccacgccc
     2041 gggaccgacg catctgcgcc gcgacgcggt gctgctgttc ctcgggctgt ggctcctgta
     2101 cggcgcgggg atcaacaaac tgaacctgca ggggtacacc ctccaccccg aggtggtcat
     2161 ggcgatcgcc gaggagggca cgttcgtcat cggccaccag gaagttccga aggagaacga
     2221 ccgattcctc ttcgacggga acgtcctgcc ggcgaagcag ccctggatct tcgcgctcgg
     2281 ggcggcggcc tacctgcccg tgcgcgccct ggggctgacc tatgaccgcg acttcttcct
     2341 ggcggaagcg gtcatcacct ggctcaccgc ctcgctcttc gcggcagtgg ccgcggcggc
     2401 ggcgctcgtt gtcgcgagac gcgtctgggg cttccgccgc cgggacgcac tccttgccgt
     2461 cctggccggg gccgcgtgca cgatcctcct cccctacgcg gggattcccc accatgacgt
     2521 cgtggcgaca gcgctgctgg tcctggcgct tgcggcgttc gaggtggcac gatcggacga
     2581 ggccgcgggg acgagcggga aggcgcggcg gcgtctcatg gcgtccgggc tcgccgggct
     2641 cttcctgggg ctgatgctcg ggttctcgat gctgccggcg gcgatcgcgc tcggcgtgat
     2701 cgtcgcgatt gccgcgacac ggaacaggtc gatcgtcgtt cccgccgcgg gcggattcgt
     2761 cctgggactc ctgccgctcg ctctctacaa cgggcactac ttccacaccc cgttcttgca
     2821 ggcgaacctc gcggggcagt acgacgacac ctatccgggt ttcgcgccgg agcgcatcgt
     2881 gcaccacctc aacgcctatc tcggcgtggg agaggtgtcg atcctcaagt acatgccgat
     2941 cctcctggcc ggcgcggccg ggctggtcct gctcgccgtc cgtggtcgcg ccccgggacg
     3001 gctcctgctg acgcttctgc tcctgcacgg gatgtatctc ctggcgattc ctgccatcgg
     3061 ctactgccag tacggcccgc gcttcctgct cccggccgtc ccgctcgccg ccttcggcct
     3121 cgcccccctg ctcgaggcgc tcgaccgctt gcagggcggg tggcggctcg cgggtgcggc
     3181 gctcgtcgcg ctcctggcgc tcgcctcgtt gatggtgaat ctcgtcggca ccgtgggggg
     3241 gacgatgtac tgcgccatcg aggagttcgc cttcccgcgt tacgtcgcgg ttctcgcgga
     3301 tccgcaggcc tggacggcga tcgcgacgca gtttcctctt ctgggattct gctcggccgg
     3361 cctgctgctg ctcggcgtcg cggggttcgg cggggtgttg ctggcgcggg gacgcgccgc
     3421 gtcgcttgac gcttccgtca ccggccgcta ggctctgtcg ccgatgaacg acgcccggca
     3481 cctgcgcgac gccatcctgg atttcatgcg gcggccgaac taccggccgg tgagcgccga
     3541 cgagttgcag cgcctgctgc gcatcgatcg ctcgcgccgg catccgttca gccgggcgct
     3601 ccgcgcgctc gtcaacgacg acgcggtcat ccgggtggac cggcatcgct acgccattgc
     3661 gggctggacg ccgacggtgc cccgcagcgg cggccgggat ggccgcggcg acggcggccg
     3721 gcgcgtggag cgccgggacg gcgggcgcca tggccgcacg gccgagccgc gcgtgcgcga
     3781 cgtgccggcg cagggcgccg cggctcccgc cgccgggccg atgcgcgccg gatcgatcgt
     3841 gaccggaaac atccaggtcc accccaaagg gttcgccttc gtcacccccg accgcggcgg
     3901 cgtcgatctg ttcatcccac caccggcggt cggcgacacg caggacggcg accgggtcga
     3961 ggcgattgtc gtccgtatgc aggcggacgg ccgggcgacc ggggacatcc gacgggtcct
     4021 ggagaagaca cgccgtcgca tcgtcggcgt ctaccgcggc gggccggacg gcggcaccgt
     4081 cgaggcctac gaccgccgct tcgaggccgg catcgccatc ccgcgcgagg gggtcggcgg
     4141 cgcggtgaac ggcctcgtgg tcggcgtcga ggtcgagcag caggcggagg aggggcgaag
     4201 cgcctggggt cggatcgtcg aggtgatcgg cccggccgag tcccccgaga ccgacctcaa
     4261 ggcggtcatc cggaagttcg ggctgcggca ggagttcccc cccgacgtca tgcagcaggc
     4321 ggcgcaggcc cccgaccggg tccccccgga ggacatccgc ggccgcgagg acttccgcga
     4381 cctgccgatc gtcacgatcg acggcgagac cgcgatggac ttcgacgacg cggtcctggt
     4441 gcgccgcctc gacggagggc attacgagct gcacgtccac atcgccgacg tggcccatta
     4501 cgtgcgcgga tcgtcgccgc tcgaccggga ggcgctggag cgggccacga gcgtctactt
     4561 ccccggaacg tcgctgccga tgctgccgca ccgtttgtcg aacgggatct gctcgctcaa
     4621 tcctcacgtg gaccggctcg ttctctcctg cgtcatgacg atcgacccgc agggacaggt
     4681 gacgcagtac cgcctcgccg aaggggtgat tcgcagcgcc gcgcgcatga cctacaccaa
     4741 cgtcgcgaag atcctggtcg atcgcgaccc gcgcgtgatg acgcagtacc gcgacttcgt
     4801 ggcgggcttc cgcctgatgg aggagctgtg cggcgtgctc aacgcgcggc ggcgccggcg
     4861 cggcagcatc gacttcgacc tccccgagcc ggaggtgatc ctcgccgcca cgggggagat
     4921 gaccggcatc atggcgctcg agcgcaacat cgcccaccgg ctgatcgagg agttcatgct
     4981 gacggcgaac gagacggtgg cgcggcacct gttccgggcg aaggtgccgt cgatgtaccg
     5041 ggtgcacgaa cgtcccgacc cgcgccggct cgaggacttc gaggcggtgg cgcacgcgtt
     5101 cggctacagc ctgccccggc cgttcacctc gatcgagccg cgctctttcc aatcgctcct
     5161 cgagcgcgcc cggggacgcc cggaggagcg cttcctgtcc cgtctgatgc tgcgctcgat
     5221 gaagcaggcg cgctactcgg agaagcggga catccacttc ggcctcgcct ccacctgcta
     5281 cacgcacttc acctccccca tccgccgcta tcccgatctc gtggtgcatc gcctggtgaa
     5341 gcggctgctc aagggcggcc cggtgcaggc gcgcgagcgg ggcgacctcg aggtgttcat
     5401 gccggaggcg gcggcccgat cgtcggtgcg ggagcgcacc gccgacgccg ccgagaacga
     5461 gctcatcgag cgcaagaaga tggtgttcat ggcccagaag atcggcgagg agttcgacgg
     5521 cttcatctgc ggcgtcgagg cgttcgggat cttcgtcgag ctccaggagc tgttcgtgga
     5581 cgggctcgtc gggatcgacg cgctccccgg ggatcgcttt cgcttcatcg agcggcagcg
     5641 catcatgaaa ggggagcggc acgggcaggt gctggcgctc ggcgatccgg tgcgtgtgcg
     5701 gctgagccgg gtcagcgccg cgcggatgga gatcgagttc gccctgctcg agcatcgccc
     5761 ggggccgagg cccgccggcg tcgcggcggc ggtgcgggag cccgccgaga cgcgggcggg
     5821 acggcgcgac gtcgcccgcg gcaagcggcg ccaggacgag cgcaagcggg ccgcggcgtc
     5881 cggcgccgcg gcccccggcc gcggccacgc gcccgcccgg cacggtcgtc gtggacgcac
     5941 gcggggcggc ggcggttcga cgggggccgg ctcggtgcgc agcggccggg ggcgccgccg
     6001 gcgctgagac cggtggcgcg ccggagacgc gcggccgctc cgcgccgcgg cgtccccgcc
     6061 gcgcgcaggc gagccgcacc ctaccgcttc ctgccgcaca ccgccgacat ggccgtcgcg
     6121 gtccgggagc gcaccctcgc ccgcctgttc gagaactccg ccgcggccct gttcgacctc
     6181 gtcaccgacc gcggcgtcgt gcggccgcag cgttcggtgc ggttcggcgc gcgggggcag
     6241 agcctcgacg acctcctggt ccgcttcctg tcggagctgc tgtacctgca ggaagtgaag
     6301 ggatggcgct tccgctgctg cgccgtcgaa aagctcgacc gtgcaggact gcgcgtccgc
     6361 ggcgcagcgg aaggggagcc gttcgactcc gagcgccatc cccgccgcca cgaggtgaag
     6421 gcgatcacct accatcgcct cggaatcaca cggtccgccg ggcaatggca ggtcaccttc
     6481 gtcgtcgatg tctgagacaa cttgatcccc ctcccgattc agggtaaaaa gcccggaatt
     6541 ggcccggcgc cgcctgccga cggtcgggcc cacacaaaac ggagcgacag ccatggaatg
     6601 gttctatgtc gccgatcagc gacaggtcgg tcctctgagc gaagcggagt tcgcgcgtct
     6661 cgtgggtgcc gggaccatcc tgggcgagac gctcgtctgg cgggtcggca tgaaggagtg
     6721 gcagccgtac agcgcgctgc cgccgatcgc tccgccaacg acaccggcgg ccggtgaccc
     6781 gggggccgac tggcccgtga cgcaaggatc gcctgtggcc gccgcctctt ccgcgatggg
     6841 gacgtgcacg cagtgcggca ggagcttgcc ggcgacggag atgctggcct acgccggcgg
     6901 ttacgtctgc ccgacgtgca agccgatgtt cgtgcagcgt ctcaaggagg gggcgcgcgt
     6961 cacggcgggg gtccgctacg gcggcttctg gatccgcttc gccgcccgct tcgtcgacgg
     7021 aatcatcctg tggatcgcga atgtcatggt cctgctgccg ttcggcctcg gcgctgccgg
     7081 gacaatgttg agaggcaccc cccccgaagc ggccgacctc ccttcccttc tggcgatgca
     7141 gggattgttg atcctgatcc aggtcggcat cgcggcgtcg tatgagattt tcttgacgac
     7201 gaagtatggc gccacgctcg ggaagatgat cctcaggctg aaggtggtga gaccggacgg
     7261 cggcagcatc ggcttcggcc gcgccgtcgg gcgctacttc agcacctgga tctccgggtt
     7321 caccctcggc atcggctaca tcatcgccgc cttcgacgac gagaagcggt cgctgcacga
     7381 tcgcatctgc gacacgcgcg tggtccgggc gtgagtccgg cctgcccggg atgccggacg
     7441 gcgctgcctc cggacatcct cggggcggag ggagaaacct cgtgccctcg atgccacatc
     7501 ccggtcgagg tggaggtgtt cccggcgctg acgcgtccgc tggaagccgc gtcggggagc
     7561 gccgtgcaat cggcggaggg ggagtccacc tgcttctacc acgccggcta ccgggcggcg
     7621 gtcccgtgcg acgcctgcgg gcgcttcctg tgcagcctgt gcgacctcga aatcggcagc
     7681 cggcacctct gccccgagtg catccaccgc tcgatccagg ggccgaaggc gacgatgacg
     7741 agcgaaagga tcctgtggga ccggctggca ctgtcccttg ccgtgctgcc gatcttcacg
     7801 ttctatttca ccgtcgtgac cgcgccggcc gcgatcttca tcgcgctgcg gcactggcga
     7861 tcccccggga gtctcgtctc ccatggccgc tggcgcttcg tcgtcgcggt gctctgcgcc
     7921 acgctgcagc tcgccggctg ggcggggatc atctacgcca tcgcgcgcgc cggcgggaca
     7981 gcggtcgaga ccgccgtcga tacggaggac tgacccggtg acgccgcccg ccccaccgcc
     8041 tctctaccgg cgcctgccgg ggcgacgacg cggcctggcg accagcgcca cgctctggca
     8101 ggcggatgat cacctgctcg tcgccgaggt gcgcttctgg accgagagct accgccgcat
     8161 ctactatcgg gacatccagg cgatcgttct cggccaggac ggacgctggc tggcgttgaa
     8221 cctgatgttc ctgacgttcg gcacgctgct cctgctcgtg gtcgctggga cccagggcgg
     8281 ctggcggatc ttctgggcct ttccggcggc gctggtggtg ctcctgggga gcgtgcactt
     8341 cctgcgcggc cccacctgcg cgtgcgaggt gacgacaccg cttgccaccc tgcgtctgcc
     8401 gctgcagcgg gtgcgaccgg cgcggcggac gctggcgctc gtgcgctccc ggatcgaggc
     8461 ggctcaggga acggcgacgc gcgaaaagat cgtcgcgcag gcggccgagg gcccgccggc
     8521 cgcgcccccg gccgcgccga tcctcctctc gcgcacgccg ccgccgctgg tgcggccggg
     8581 gcagccgcgc atgcacgccg tgttgtgcgc cgccctggtg ctcgacgcca tggtgaccgt
     8641 ctatcaatgg ccgcacggca acacggtgat cgactccgtc gccgtcgtca ccttcttcgg
     8701 ctgcgtcctc gcctccatct tcgcgatcgt cgctcagaac cggaccgacc tgggcccgga
     8761 gttccgctgg ttcggggtga ccgccctcgg ggcgcagatc ctgctgttcg tggccgggtg
     8821 gatcttcctg ttcgccggca tgatcaagca ggccggcgcc gggggggcgc cgcaatttcc
     8881 ggccctgttc gcatcgggga ccgaggcgat tggtccctac gagatcgcga tgcagtcgat
     8941 cctcgcactc tggggactgg tggtgctcct gcgctggcgt cgcgcgcgga aaaccgcgtg
     9001 gatcagcctg acgacctgag gctcaggcgc tgcctgcatc acgccacgcg cgaggcggtg
     9061 gcgcgctgcc cgcgctgcgg ccgctccttc tgccgcgagt gcatctcgga gcacgacggg
     9121 ctctggctct gcgccgcctg cctcgcagcg gaaggggagg ggagcgcggc gcggcgcggc
     9181 cgcctggtcc tggtctggag cggcctgcgg gtcggggccg ggctgttcgt cgcctggttc
     9241 tacttctacc tcgtcggcca ggcgctgctc gccttccacg acccgatcca cccgggtggg
     9301 gatcgccccg gggtcacggc cccgtgaggc ggagcggcgg gggaatcgac atcctcgacg
     9361 acgccgttcg cctgctccgc gccgccccct ggtcggccca cctgtgcttt cacgccggca
     9421 gcctgccgtt cgtcttcggc ctcctgttct tcgtcgtgga catgcgtcgg agcgccctcg
     9481 cccccgagca cgccggatcc gcggcgctgc tgatggcgtt gctctacctg tggatgaagg
     9541 ggtggcacgc cgccgccgcg gtccgcctgc ggcgggcgct tgcgggcgga gacgcggagc
     9601 gggagcgggg gatggcgcgc cgggtgctcg cccagtgcgc cattcagccg tggtccctgt
     9661 tcctgctgcc ggcggcgctg gtgctgacgc tcccgtttgc gtgggtgtac gcctactatc
     9721 acgccgcgac gctgacctcg gacgcggcgg cggcccgcga gcagtcagcg ttccgtccga
     9781 tggagaacca tctcctcgtg ctgattctct ccgccggcgc gttcgtcgtc tggaccaacc
     9841 tgctcgtcat cgtcctcgcg ctgccgttcc tgctgagcac ttttttcggg atcgagacgc
     9901 cgttcagccg caccccgatg cttctcttcg actcgaccgt ggccctgctg gtgtcgggcc
     9961 tcgcctacct cgtgatgggc ccggtggcgc tcgccgccta cctcgtgcgt gccgagcggg
    10021 ccgagtcggc ccgcaccggg agggacctgc tgcgcggcat cgacgcactg ccgccgccgg
    10081 tcacgggggg gcgggcggtc gcgtgcctgc tggccgggct cctcggggca gcatgcgccg
    10141 cagcctcggc ggaggccgcc gtgccggtga tcaccgaggc gccggcgacc gtcgcaaagc
    10201 ccgcgggcgg gatcccggtc gccgaactcg acgacgcgct gcggcgcgcc accgcgcggc
    10261 cggagtacct gtggcgcatg ccgcgtccgg cgcccccgcc ggacgaggat gagccggggc
    10321 tgttcgggcg cttcggccgg accatcgcct ccacgctgcg ctcatggtgg gacgggctca
    10381 gccgcgccgt gcaacggctg gcccggtgga tcgaccgcca tttcgagacc ggcgagccgg
    10441 cggagcatcg cgtggggatg cccggatggc cggcgggcgc ctcgtgggcg ctcctgggag
    10501 tgatcgttct cctcgtggtc tccgccggtg tctcctggtg gaggcgccgc acgaagagcg
    10561 ctgaggtccg cgcgaccgtg gtggctccgc gcgggatccc ggtggacctc tcctccgcgg
    10621 cgccgaccga cgccccctcc gacgagtggc tcgagcgctc gcggggcctt ctggagcgcg
    10681 gcgagctgcg cttcgcggcg cgcgccgtgc acctcgccac cctggcgctt ctcggcgagg
    10741 cgcgcctcct gacgctcggg cgggcgcgca gtgaccgcga ctacctcgtc gagctccggc
    10801 gccgccgtcc cgatcggccg gagatcgcgg atctcttcct ggacggcgtg cgcgacttcg
    10861 agcgggtctg gtacgggcgc catccggcga gcgtcgatct ggtgcggggg ctcggggagc
    10921 gttgcgagag gctgaggcac tatgcgcagg gataggctgg cgctcgcggc gctcctgggg
    10981 ctcgcggtcc tcttcgccgc cggcctcgcg ctcgtggcgc gcgaacggta cgcgcgtggc
    11041 gacgggtatc ccgccgggtc gtcattccgc accgatccgc tcggcacgaa agcgctccac
    11101 gacgccctgc tcgagatcga gggcctgcgg gtggccaggc acgtcggtcc ggccaccgat
    11161 ctgcgcggag gggaggggac ggtggtcctc ctgctcgagg tctcggaccg gctcggcacg
    11221 gccgacccgg tgctcgtccg cgccgtggag gcggtcggcc tcgccggcgg ccgcatcgtc
    11281 gtcacgctcg cgaggtcgca gcgctcctgg ttcgaggacg aggacgatga ggatccgaag
    11341 gagagcgaga agaagcggga ggatgagagg gcggagcagg gggagcgcga gccccaggcc
    11401 tctttcgccc ctccgctcag cgcccgctgg ggattcgcgg cccgaccctg tccgtccgaa
    11461 ccccacgcca cgatcgcggc cgtgccgatc gcgacggagg aatccagcgg ggatctgcca
    11521 tggcgcggcg gcgactgcct ggcggatctg acgccggcgt ggcgttccct ttaccgcctg
    11581 ggagcgcatc cggtcgtggc ggagcgatcg ctcggccgcg gctcgatcgt gctgctcacc
    11641 ggcggctacg tcctgagcaa cgaggggctc ctcttcgacc ggcggccggc ggtcctgtcg
    11701 cgcctcatcg gaacggatgc ccgcacgatc ctgttcgagg agtcgcacca tggggtgcgc
    11761 cgcagggccg ggatcgtggc tctgctgggt cgctaccgca tgcgcggagc gctcgcggcg
    11821 gccgcgatcc tcctcggtct ctggggctgg tgggccgggt cgccgctcgt tccggccgcg
    11881 ggaggggcgg agacggagcc gcgcagcggc aaggaggcgt cgctcgggct cgtgagcctg
    11941 ctgcgccgcg gcgtgccgcc gaagcgactg atggagacct gcctggagga atggcgccgt
    12001 gccttcgcgc gggcgcgtcc ggcggccgcg gccgcgctcg cgcggctgcc gccgccgggg
    12061 gaggacatcc cggcagcgta ccgccgggcg cgggaggcgg tgaatccgaa cgggggagac
    12121 gatgatcgct gagaccgagc gcctgcacga agtcctgacc cgggcacgcg aggaggtggc
    12181 gcgcgtcatc gtggggcagg cggacgccgt cgatcaggcg ctggccgtga tcctcacggg
    12241 gcaccacgcc ctcatcgagg ggatgcccgg cgtggccaag accctcctgg tgcggagcct
    12301 cgcagcggtc ctggggtgcg agttcgcgcg catccagttc acccccgatc tcatgccctc
    12361 ggacatcacc ggcacgaacg tcttcaacct caagaccagc gagttcgtct tccaccccgg
    12421 gccggtgttc acggcgttcc tgctcgccga cgagatcaac cgggcgccgg cgaagaccca
    12481 gtcggcgctt ctccaggcca tgcaggagcg ggcggtcacc atcgaccgcc agacgcgccc
    12541 cctgccgccg cagttcaccg tcttcgccac gcagaacccg atcgagttcg aggggaccta
    12601 cccgctgccg gaggcgcagc tcgaccgctt catgctgaag atccgcatgg atcccccggc
    12661 ggccgacgag gagctggccc tggcgcggcg cctcctcggg gacgaggcgc cggaggcggt
    12721 gctggcggcc ggagggatcc gcccggtcgt ggggcgcgag gacctcgtcc ggctgcagcg
    12781 cgccctgatg gccgtgacgg tgcgcgacga gatcctcggc tacgccgtcg agatcgtccg
    12841 gcgcacccgg gcgcacgacg ccatcctcgt gggcgccagc ccgcgagggc tcgccgcgct
    12901 cctgcatgcg gcgcgcgccc aggcggcgat cgccgggcgc gacttcgtga cccccgacga
    12961 cgtgcgccgg atcgcggtgg cggtcctcgg gcaccggctg gtgctgcggc cggagtacga
    13021 gatcgaaggg gaggagatcg gcgcggtggt cgcgcgggtg ctggcggagg tcccggtgcc
    13081 gcgatgatcg cgccgcgccc gcgcctcctg ctcgcggtcg gagggatcgc cctgccgctg
    13141 gcggcgctcc tgcctgcgct gccggagcga tcgggggagg cggtgctgct ggcgctggcg
    13201 gcgatcgtcg cggccgacgc gctcctcggg ccgcgccgcc tcgcggggat cgaggtgctg
    13261 ctgccttcga ccgcgcggtt cctgcgggat cgcgaggggg agatcgcgat ccgcgtccgg
    13321 cacctgaacc cgacctcccg gctcgtcgcg gtcgggctgt cgctgccgcc cgcgctcgcg
    13381 tcccggagcg acccgctgcg tgtccgtctc ccggcaggac cctcggaggc cgtcttcgcg
    13441 tggccgctca ggccacggag ccgcggccgg ttccggatca ccgcgctgca cctcgaggcc
    13501 tcctcgccgc tggggttctg gggggcccgg cgcgcggtcg aggtcgcgac ggaggtgcgg
    13561 gtctacccga acctcctcgc cgagggacgg ggagcggcgg cgctcttcct gcggcgcagc
    13621 cgcgtcggcc tgcatgcgct gcgacggatc ggcaaggggc gagagttcga gagcctgcgc
    13681 gactaccagc cgggggacgg cttcgaggac gtccactgga aggggactgc caaacgcggg
    13741 cgtccggtca ccaaggtgtt ccaggtggag cgcacccagg aggtgtacgc aatcgtcgac
    13801 gcgtcccgtc tcgccgcccg actgcagggg gatccgccgt ccgcggcgat cgagcgtttt
    13861 gtcagcgcgg cgctcctcct ggcgcgggcg acggaacggc agggggatct gttcggcctc
    13921 gtggccgcag ccgacagtgt gtcgatgttc ctgcgggccg gccgcggccg ggcccacttc
    13981 gacgcctgcc gcgaggcgct ctgtgctctc gaggcgcgac cggccacccc ggacttcgac
    14041 gagacggcca cgttccttct gtcgcggctg cggcggaggg cgctcctcct cttcctcacg
    14101 gacctcgacg acccggtcct ggcggagggt ttcctgcgct gcgccggggt gctggcgcgg
    14161 cgccacctgg tggtggcctg cctgctgcgg ccgcacgggg cgcgtccgct gttcacggga
    14221 cccgacccgg ccgatccgga cgatgtctac cgccggctcg ccggccatct cgtgtggagc
    14281 gatctccagg aactgacgcg gggagcgcaa cgcaaggggg ccgccgtcag cctgttcgag
    14341 ggatcctccc tgccgacgga cgtggtcgga cgctatctcg aggtcaagcg gcggcaggcg
    14401 ttgtgatcct ggatctgccg cgtttcctcg cctccgagcg ccccttctgg agcgagctcg
    14461 aggagtggct cgaccgtctc gagacggacc cgcaccggac gatgagcctc gaggaggcgc
    14521 ggcgcctgca ctacctgcac cagcgagcct cggccgacct cgcccggatc cagtcctccg
    14581 ccgcagagcc gcagacgcgc gcctggctcg aggcgctcgt cggccgcgcc tacggcgaag
    14641 tgcacgagac gcgcgaccgc acgcaccgcc ttcgcccgct gcggctgttc ctgcacgact
    14701 ttcctcgcgc ctttcgccgc cgcgccgggg catttgccct ggcatgcggc atgctgctcg
    14761 ccgggacggc gctcgggtcg atcgccctgc tcgtggacga cggggcgcgc gaggtggtgc
    14821 tgccggcgga gctcgcctcg ctgtcgccct ccgaacgggt gcacgacgag gagacccgcc
    14881 gcaccgaccg gctccagggg cagcggtcgc gcttctcggc gatgctcatg acgcacaaca
    14941 cccgcatcgc cctcttctgc ctcgccctcg gcgtcaccta tggcatcgga acggcgatcc
    15001 tcctgttcca caacggcgtc gtgctcggcg cggtggcggc cgactacgtc gcggacgggc
    15061 agacgcggtt ccttctcggc tggctgctgc cgcacggcat cgtcgagctg ccggccatcg
    15121 ccatcgcagg ccaggccggg ctcgtcctgg cgtcggccct cctgggccgc ggtgacggga
    15181 ccccactgcg cgagcgtctt gcggtcgcgg gacgggacgt cggggcgctc gccgccggcc
    15241 tcgccaccct gctgctctgg gccgggctga tggagtcgtt cctgtcgcag caccacgagc
    15301 cggtgcttcc ctacgccctc aagatcggca tcggcgccgt cgagggagtg ctcctcgtcc
    15361 tcttcctgag cctcgcggga cggcggccgt gagccgcgat gccgtcacca tccggacccc
    15421 ggaggggatc gtcttcgccc ttccgctggc gggccccggg gcccgcttcg tggcctgggg
    15481 cgtcgatctc gcctgcgtcg gcgccgtggg ctcgctcctc gcgacggcca tccgggggct
    15541 cctcgggttc cagccggaca tcgcggaggc ggtgggcgcc gtcctgtatt tcggcctgtc
    15601 gatcctgtac ggcctggtgc tggaatgggt ctggcgcggg cagacgctcg gcaagcgtct
    15661 ggtcgggttg cgcgtcatgg acgaggaagg gaggcggctg cagccctcgc aggtcgtggt
    15721 acgcaacctc ctgcgatcga tcgacgtgct gccgatcttc tacctcgtcg gcggcgcggc
    15781 gatgctcgcg agccgccact ggcagcggct cggcgacctg gtcgcctgca ccatcgtggt
    15841 gcgggccgcg cggaccgatc cccccgaccc ggacgcgctc cgcagcgaca aatacaattc
    15901 cctgcgcggg cgcccccgca tcgaggcgcg cctgcggcag cgcatctcgc cggccgaggc
    15961 ggcgctggcc gtccaggcga tcgtccgtcg cgacgccatc gagccggcgg cgcgcgtcgc
    16021 catcttccgc cgcctggctc agcgcttcgt cgccgcggcg gagatcgagc cggcgctgtt
    16081 cgagtcggtg cccgacgagc aggtggtccg caatgtcctg gagatcgtcc tgacgcggcc
    16141 gggtcgggcg ggggtgtcga cgtgaatgcg cgcatcgcgg caccgtcttg atccccccgg
    16201 cgattccgga gtaaaaggcc ccgccatgga atggttctac gtcgagaatc agcagcgggt
    16261 cgggccggtg agcgaggggg agttcgcgcg tctcgtcggc gcgggaacca tccgccgcga
    16321 aacgctggtg tggcgcgccg ggatgaagga ctggcagccg tacgggtcgc tcctgccgcc
    16381 gcccccaccc gcgccgcccg ccgcctccgg aggaccgatc ccttccacgc cggtcgccgg
    16441 gtcgccgcag gcgggatcgc cgggagtgcc gtcgcctcat tcctccgcgc cggggggggc
    16501 cgccgcgacg gcgcggggcg ggacgttctc cgcagcctcc gcgaccatgt cgccc
//
LOCUS       DASRIQ010000623         4249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1025187, whole genome shotgun sequence.
ACCESSION   DASRIQ010000623 DASRIQ010000000
VERSION     DASRIQ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025187"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFD06_15480"
     CDS             complement(<1..347)
                     /locus_tag="VFD06_15480"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HZN04985.1"
                     /translation="MRTEARGFALITAALAMALAALPPAARAGEPSAARAAGAAAPDA
                     AGREEAALAIEEDLMCHCGCTDLTVRVCNCGVAAGIKDDIRDRLARGQSPAEVEAAYV
                     ARFGEQIRSAPTRR"
     gene            complement(337..2328)
                     /locus_tag="VFD06_15485"
     CDS             complement(337..2328)
                     /locus_tag="VFD06_15485"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HZN04986.1"
                     /translation="MPELGTFALLLALVTAGWSALASFNGWLSASGPLVRSGERALYA
                     TWGLVLGAAVLLEALIFTDRFDLEYVASYSNRALPPVYKVAALWAGQSGSLLFWLLIL
                     CSYGAALLLLNRRRNRPLMPVVTTTMAVTAFFFLLLVNFMARPFVGLTHPPADGMGMN
                     PQLQNPFMAIHQPCLYLGYVGLTVPFAFAVAALATGRLSDSWFRTTRRFTIISWFILG
                     TGILLGGWWAYVELGWGGYWAWDPVENASLIPWLTATAFLHSVMIEERKKMLRVWNMT
                     LVIVTFLLSLLGTFITRSGVISSVHSFTKSDIGPMFAGFLGLATIVSVALLLHRLPML
                     QSEHRLDSFVSRESSFLFNNLLLVGAAFAVLWGTLFPILSEWVRGVKITVGPPFFNEI
                     MVPLTLALLTLAGICPIIAWRRATPDRLLKKLRLPLGAMLALAVGLLAAGMRDPYALV
                     SFSLCGFVAATTILEFAAGTRARRSATGEPWGRAFVRLVDRNKRRFGGFIVHLAVVLI
                     LVGVTGSSVFKREQVASLKRGESFSIGRFTLTLDEMVSRDTPNHEFLGARLLVAESGR
                     QVGTLLPGQNFYKAGQNPSSEVDIRSTLSEDLYLILTGFDPQEGRATVKAYLNPLVAW
                     IWIGGAVLILGSWLAILPDLRERRRAAEARLRAIESYAH"
     gene            complement(2355..2729)
                     /locus_tag="VFD06_15490"
     CDS             complement(2355..2729)
                     /locus_tag="VFD06_15490"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZN04987.1"
                     /translation="MAGVIAGFVILFNVGLSGSMVYYLTVSEFLDGDRAHDLGENFRV
                     NGRAVPGSIERSPSGLGCRFRMTDGTKELAVTYDREIPDTFVDNSEVVVEGRLGKDGV
                     FEARTLLAKCPSKYESAAPSRS"
     gene            3051..3527
                     /gene="greA"
                     /locus_tag="VFD06_15495"
     CDS             3051..3527
                     /gene="greA"
                     /locus_tag="VFD06_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZN04988.1"
                     /translation="MLSESRARLEKELQTLEHEYRHELPKEIQRAKALGDLRENAEYQ
                     AALERQHYVQARIGQVQAQLRTLAMIPLDSLPRDRAALGSTVTVRDTQAGKEITYELV
                     IPEEADLSKGLVSATSPIGRALVGHQEGDEVTVQLPAGTRGLEILKLITIHDKEKQ"
     gene            3524..>4249
                     /locus_tag="VFD06_15500"
     CDS             3524..>4249
                     /locus_tag="VFD06_15500"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZN04989.1"
                     /translation="MRDARRPGFAPAAAVALAALFAACAPAEDPEKLFADLQNPDSEA
                     RQEASERIDAIVGRGDPGPFVRGLESPSLPVRAQAILQIARMSSPEARTALRSLLDRD
                     RRMMLPFNPIRLKPMQEPQDSRILVAHLIRRGGGDPEAIDVLLRGSEENQTVDLLGDT
                     CLAVGALADPKGIPFLEKAAMHPDTTVARAAAQALHQFAGPESLAALGRLLDNPSSEV
                     RGEVVAGLTARQEDGVEALLKRVA"
BASE COUNT          738 a         1419 c         1525 g          567 t
ORIGIN      
        1 cgccgggtcg gtgccgatcg gatctgctcg ccgaaacggg cgacgtaggc ggcctccacc
       61 tccgcggggg actgtccccg ggcgagccgg tcgcggatgt cgtccttgat cccggccgcg
      121 acgccgcagt tgcagacgcg caccgtcaga tcggtgcagc cgcagtggca catcaggtcc
      181 tcttcgatcg cgagcgccgc ctcctcgcgg ccggccgcgt cgggagccgc cgctccggcg
      241 gcccgcgcgg ccgaaggctc gcccgcccgc gccgccgggg gcagggccgc gagcgccatc
      301 gcgagcgccg ccgtgatcag ggcgaagccg cgcgcctcag tgcgcatacg actcgatggc
      361 gcgcagccgc gcctcggcgg cccgccggcg ctcccgcaga tcggggagga tggcgagcca
      421 ggagccgagg atgaggaccg ccccgccgat ccagatccag gcgacgagcg ggttgaggta
      481 ggccttgacg gtggcgcgcc cctcctgtgg gtcgaagccg gtcaggatca ggtacaggtc
      541 ctcgctcagc gtcgatcgga tgtccacctc ggacgagggg ttctggcccg ccttgtagaa
      601 gttctgcccg gggaggagcg tcccgacctg ccggcccgat tccgcgacca ggagccgcgc
      661 gccgaggaac tcgtggttcg gcgtgtcgcg gctgaccatt tcgtcgagcg tcagggtgaa
      721 gcgtccgatc gagaacgact cgccgcgctt caggctcgcc acctgctcgc gcttgaacac
      781 gctcgagccg gtcacgccga cgaggatgag gacgaccgcc aggtgcacga tgaagccgcc
      841 gaagcggcgc ttgttgcgat cgacgaggcg gacgaaggcg cgcccccatg gctcgccggt
      901 ggcactgcgg cgcgcccggg tcccggccgc gaactccagg atggtcgtgg ccgcaacgaa
      961 gccgcacagc gagaacgaca ccagcgcata cgggtcgcgc atcccggcgg cgagcagccc
     1021 cacggcgagc gcgagcatcg ccccgagcgg cagccggagc ttcttcagaa ggcggtccgg
     1081 cgtggcgcgc cgccacgcga tgatcgggca gatgccggcg agggtgagca gggcgagcgt
     1141 cagcgggacc atgatctcgt tgaagaacgg cggtccgacg gtgatcttca cgccgcgcac
     1201 ccattccgag aggatgggga agagcgtccc ccagagcacg gcgaacgccg cgccgacgag
     1261 gagcaggttg ttgaacagga agctgctctc gcgcgacacg aacgagtcga gccggtgctc
     1321 cgactgcagc atcggcagcc ggtgcaggag caacgccacc gacacgatcg tggcgaggcc
     1381 gaggaatccg gcgaacatgg ggccgatgtc ggacttggtg aacgagtgca ccgaggagat
     1441 gacgccgctg cgggtgatga aggtcccgag cagcgacagc aggaacgtga cgatcaccag
     1501 cgtcatgttc cacacccgca gcatcttctt gcgctcctcg atcatcaccg agtgcaggaa
     1561 ggcggtggcg gtcagccacg ggatgaggga ggcgttctcc accggatccc acgcccagta
     1621 gcccccccag cccagctcga cgtaggccca ccagcccccc agcaggatgc cggttccgag
     1681 gatgaaccac gaaatgatgg tgaagcggcg cgtggtgcgg aaccacgagt cggagagtct
     1741 cccggtcgcg agcgcggcga cggcgaaggc gaacggcacg gtgagcccga cgtagccgag
     1801 gtagagacac ggctggtgga ttgccatgaa cgggttctgc agctgcgggt tcatccccat
     1861 gccgtccgcg gggggatggg tgaggccgac gaacggccgc gccatgaagt tcaccagcag
     1921 caggaagaag aacgccgtca ccgccatcgt ggtcgtgacc accggcatca gcggccggtt
     1981 gcgccggcgg ttcaggagca ggagcgcggc gccgtaggag cagaggatga gcagccagaa
     2041 gaggagcgag ccggactgtc ccgcccacag cgccgccacc ttgtacacgg gagggagcgc
     2101 ccggttcgaa taggaggcga cgtactcgag atcgaaacgg tcggtgaaga tgagcgcttc
     2161 gaggaggacc gcggcaccga gcaccagtcc ccaggtggcg tagagggccc gctcgccgct
     2221 gcgcacgagc ggacccgacg cgctgagcca gccgttgaac gaggcgagcg ccgaccagcc
     2281 agcggtgacc agggccagga ggagggcgaa tgtgccgagt tcgggcatgg gggcctagcg
     2341 gccggccgcg gggctcacga ccgcgacggg gcggccgact cgtacttcga agggcacttg
     2401 gcgaggagcg tgcgggcctc gaagacgccg tccttgccga ggcgtccttc caccaccacc
     2461 tccgagttgt ccacgaaggt gtcggggatc tcgcggtcgt aggtcaccgc cagctccttc
     2521 gtgccgtcgg tcatgcggaa gcggcagccg agtccgctgg gggagcgctc gatggagccg
     2581 gggacagcgc ggccgttgac ccggaagttc tcgccgagat cgtgcgcgcg gtcgccgtcg
     2641 agaaactcgc tgacggtcag gtagtagacc atcgagccgc tcaggccgac attaaatagg
     2701 atgacgaagc cggcgatgac gcccgccaca gccagccaga ttttcggaga aaaccgccct
     2761 gagggccgca tgcctcccct tcggaaatcg aggcccggat gataacgaag cggcttgcgg
     2821 cttgacaacc tcgttcggct gattacattt gcgggcagat cgcctccata tgcgttctgc
     2881 cagatgcaac atgctatttc tggtagaacg gatagcgagg ccaatgaccg aatcccgaat
     2941 ccttccacgc gaacccatcc aaagccggag ggccgaaagg ccctccaccc gcctttttga
     3001 caggcgccgg gagcgggtgg tacttttcgc cgccggcgga ggtcaggtcc atgttgagcg
     3061 aatcccgcgc gcggctcgaa aaggagctgc agacgctcga gcacgagtac cgtcacgagc
     3121 ttcccaagga gattcagcgg gccaaggcgc tcggagacct gcgcgagaac gccgagtacc
     3181 aggcggccct ggagcgccag cactacgtcc aggcgcgcat cggccaggtg caggcacagc
     3241 tgcgcacgct ggcgatgatc ccgctcgaca gcctgccgcg cgaccgggcc gcgctcggat
     3301 cgacggtcac ggtgcgcgac acccaggccg gcaaggagat cacctacgag ctggtgattc
     3361 cggaggaggc ggatctctcg aagggactgg tgtcggccac gtcccccatc gggcgggcgc
     3421 tcgtcggaca ccaggagggg gatgaggtga cggtccagct cccggccggc acgcggggcc
     3481 tcgagatcct caagttgatc accatccacg acaaggagaa gcagtgaggg acgcccgtcg
     3541 ccccggcttc gcgccggccg ccgccgtcgc gctcgcggcg ctgttcgccg cctgcgcccc
     3601 ggccgaggac ccggagaagc tgttcgccga tttgcagaac cccgacagcg aggcgcgcca
     3661 ggaggcgtcg gagaggatcg acgcgatcgt cggtcgcggc gaccccggcc cgttcgtccg
     3721 cggcctggag agcccgagcc tgccggtgcg ggcgcaagcg atcctgcaga tcgcgcgcat
     3781 gtcgtcgccc gaggcgcgca cggcgctgcg gtcgcttctc gaccgcgacc ggcgcatgat
     3841 gcttccgttc aatccgatcc ggctcaagcc gatgcaggaa cctcaggatt cccggatcct
     3901 ggtggcgcac ctcatccgcc gcggcggcgg cgaccccgag gcgatcgacg tcctgctccg
     3961 ggggtcggaa gagaaccaga cggtcgatct gctcggtgac acctgcctcg ccgtcggcgc
     4021 cctcgccgac cccaagggga ttccgttcct ggagaaggcg gcgatgcatc ccgacacgac
     4081 ggtggcgcgg gcggcggcgc aggcgctgca ccagttcgcc gggccggagt ccctcgccgc
     4141 tctcggccgt ctcctcgaca acccgtcctc cgaggtgcgc ggcgaggtgg tcgcaggttt
     4201 gacggcgcgt caggaggacg gtgtcgaggc tctgctgaag cgcgtggcg
//
LOCUS       DASRIQ010000624         9219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2326411, whole genome shotgun sequence.
ACCESSION   DASRIQ010000624 DASRIQ010000000
VERSION     DASRIQ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326411"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VFD06_15505"
     CDS             <1..1297
                     /locus_tag="VFD06_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04990.1"
                     /translation="LVTARAYLTGTLGWPDPSPGPERVPVFVSRLGAGLEGYAAPPPA
                     GARPGAPRSIAIDAALARERILPAVLHQTAHLSLADFGAAPEWWSEATASYLALVGAG
                     DRHAREAIGARLEHAAEGLATDQPAAMEGGLLWPLFLSERASDPTFVRQIWQAQKDLR
                     LDPLLAADHVLRRRMGFGVAGALREMGVWNLFTGRRDDGAHYPGGRDFAEAPLLPIAA
                     DLPGTAGPLAPIEPTGSLSLRIPADRARGALSVGVDGRGGRPAADLLIFYRSEGAQPV
                     LVPVDLSAGSATVPIPWTDAREAWILLRNQATASDEGASHFDLRFDLDPRVPFDLAAF
                     SAAALGHGIVLEWTTAAERGLLGWNVYRSESPSGPFVRLNGVAIPAYGDGTADTGYVF
                     ADEGVRPGRRYYYQVEGITSLGLPERSHTASARIEAPHQ"
     gene            complement(1341..3089)
                     /locus_tag="VFD06_15510"
     CDS             complement(1341..3089)
                     /locus_tag="VFD06_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZN04991.1"
                     /translation="MRSTRRVAAAVLAAAVLSAAGAGCDRGRPEPAGAGSGPRAADSA
                     PRSSGWVEWSDAGAGAGVRFRHVNGARGRKQMLETLGAGVCVFDADGDGRQDLYFVQG
                     GAIPGDAAPSPEPVAGFYRNLGGGRFEEAAAAAGVAATGHYGFGCVAGDIDGDSDRDL
                     FVAGYGSNRLYVNLGKGRFEERAAAAGVTGGGWSTSATLFDADRDGDLDLYVARYVAY
                     NLSQDLYCGENRPGYRTVCHPKNFDAEPDLFYLNRGDGTFVDATRSAGLHETSGKGLG
                     VVAGDVDGDGDDDLYVANDDTPNFLWRNRGRGVFEEIAQVAGCALSEDGIPQAGMGTD
                     MADYDGDGRPDLFVTNLAEETNELYRNDGDGAFSNRTSESGLGPPSLLMLGFGTFFFD
                     ADFDGDLDLYVGNGHIIDNIALYSDTITFEMPSQLYRNLGGGRFDLPPPSGPFAGRYV
                     VRGAVPFDIDDDGDEDIVASQNNGPALLWRHDGPVAGRSLVVMLQGRAPNRDAVGAVA
                     VLASGGRRQLRMARTCMSYASQGDRRLFFAFDPAAAPYHLSVRWPGAPNEEHFSITAP
                     GPITVQQGTGRSTPSR"
     gene            complement(3086..4300)
                     /locus_tag="VFD06_15515"
     CDS             complement(3086..4300)
                     /locus_tag="VFD06_15515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN04992.1"
                     /translation="MISLRGAGRVPAGCAALAILLAACPSGERPEPQGPAAPAAAPEA
                     DAAAREGMRLLQSGAVDAAEPLLVRALAARPRDARLLEAVGQVYARSDRFGQAETSYR
                     QALEADPASPGARLGLARLLGDMGRNAEGLEVLAPLLTDRGDAPPVLLQEARLRLRAG
                     DSAGGLKAARRAAEREPRSAEAQYLIGLGLEAAGEREEALAAFERAAEIDPGHLGALS
                     HVRTLAARLGRAEKAAQAGARHQAALDRRRVDERVRSHRIAGVEAFNRQEYGRALEEF
                     RIIAREDPADPQAQLFIGSSLLALDRRDEARRALDRSLQLDPRGERAWLEMGRLLALE
                     NRLDEAVTALRRAAEIEPEFAEPHYFLSGILQARGDAGGARSEMARYEELRRRTPDGG
                     MQLLDPRPGAQP"
     gene            4383..6335
                     /locus_tag="VFD06_15520"
     CDS             4383..6335
                     /locus_tag="VFD06_15520"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002447.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZN04993.1"
                     /translation="MEGFKDECAAAIREQVASLYGAGPEKIVVEWPPQPGMGDLASPV
                     PFELARRLKRPPRAIAQELCDRFPRLPWLKRVEVGGAGYLNVFVDRGMALRALARDLH
                     APEARPSGDRIIVEHTNINPNKAAHIGHLRNAVLGDSLARSLRFLGHEVEVQNYIDDT
                     GVQVADVLIGFRHILGMGLEAAQAIPDPIDRFCWDLYAKVGEWYEADPKRRALQAETL
                     KSLERREGEIGRFAAWLTRAIVSRHLDTMDRLHVRYDLLPWEGDILAYRLWETAFALL
                     KEKKAIVRVESGERAGCWVMALDEERFPDLKEAEKILVRSNGTVTYVGKDIAYQMWKF
                     GLLPSDFRYEVFRREPDGHAIWSSTSGPGDPAAPRFGRARRVVNVIDVRQSYLQAIVA
                     EGLKALGHKEEAERSVHFSYEMVALTPQSAERLGVPLSEEDRGRPYVGMSGRKGLGIK
                     ADDLIDALQAQALKEVRARHADLEEAEAGALARAIAVGALRYFMIKFTRNKVLAFDFD
                     DALSFEGETGPYLQYAAVRASGIFERMSASGGPGETEAGRLAIEAAFDVPEGPEAEEH
                     WALAHLIARFREAASQAAASLELSLLAKFAFGLAQRFNTFYHRFPVMKEPDPSWRAAR
                     VTLTWLFLAQMRRACSLMGIPIPDRM"
     gene            6337..8151
                     /locus_tag="VFD06_15525"
     CDS             6337..8151
                     /locus_tag="VFD06_15525"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HZN04994.1"
                     /translation="MTRHVGAPRRLAALVVLLIATPAAALGQEILPFSEVKAGMSGVG
                     LTVFSGTRIDEFKVEVIGTLENIAPRRNLILTRLSGGPLQETGVLSGMSGSPVYFNGR
                     LAGAVAYTWGFAREPIAGVVPIQEMLSIETREPPATRTSRLPAGAGPAGLKWLTDPAA
                     IPAHFASWFDRLPGGPAPEGLAPIGAPLLVRGVPGDVVDLLRPGLRRAGLLPVQGGSI
                     SKSEKGADAAGLLPGSGVGIALVQGDVEIAAVCTVTHRDADRLYACGHPLLNLGPTEL
                     AMTTASVSGLMPSLESSFKFASSGGEVGVFRQDRSTGVFGYVGKKARTIPVRLDLQPE
                     RGRPKRMAFEVADDPFLAPYLLYAALNAVFSSEEKDYGTVSFTYKEGSQIRVEGEEEI
                     VLRNLFAGDLARLYASGTVAFLTQVLLDSEYRSVRIAGIDLVLGTSDERRTARLERAW
                     VSRDRVRPGETIEVSIGLKPFRGPELTREMRLTVPPEVPPGRLLLQVGDASALARTDP
                     EAGDDFVPRDLRQLVWLINHLRRNDRLYAVLVRADNGLVFQGERLPNLPPSVAQVMAR
                     PDTRGNVLRVQYRGVAEESLSLPYAIDGYRLIAIDVEE"
     gene            8153..>9219
                     /locus_tag="VFD06_15530"
     CDS             8153..>9219
                     /locus_tag="VFD06_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN04995.1"
                     /translation="MSRSLRSPCAAALLWLLAGAAPARAVSPVLWTTETLDDFEKGKP
                     DGIALGAPGELTLSPALLGLNVPALEQSSEPFLWSVAVDSKGTVYAGGGRNGTVYRVP
                     KGGAGSAWFESGALAVHAIVVDKSDAVFAATLPEGRVVRITGEGKGETWYQPEDRYIW
                     DLALGPKGELYAATGERGIIYRITGLGKAEVFFDSPEFHIVSLAVDAQGNVLAGSDGK
                     GLLYRITPQGKASVLYDSPLREIAAVAVDPKGAIYAAALGTEGEAPTLPLLIPQTMPI
                     GREQVPGLPGQPPVQVPGIAEEPSARVTVTTVSASLVPTGPPPRSEVYRVDPDGTVVT
                     LWSSPTEVAYALSIDAAGRPVL"
BASE COUNT         1260 a         3323 c         3340 g         1296 t
ORIGIN      
        1 gctggtcacg gcgcgcgcct acctcaccgg cacgctcggc tggcccgatc cttcgcccgg
       61 gccggagcgc gtcccggtct tcgtcagccg gctcggcgcg gggctcgaag gttacgccgc
      121 cccgcccccc gccggcgccc gtccgggagc gccgcggagc atcgccatcg atgcggccct
      181 ggcgcgcgag cggatcctgc cagcggtcct gcaccagacg gcgcacctga gcctggccga
      241 tttcggagcc gcccccgagt ggtggagcga agcgaccgcg tcgtatctcg ccctcgtcgg
      301 ggccggagac cgacatgcgc gcgaggcgat cggcgcccgc ctcgagcatg ccgcggaggg
      361 gctcgccacc gaccaaccgg cagcgatgga gggagggctc ctctggccgc tcttcctctc
      421 ggagcgcgcg tccgatccca ccttcgtgcg gcagatctgg caggctcaga aagatctgcg
      481 cctcgatccg ctcctcgccg ccgaccacgt gctgcgacgg cggatgggat tcggcgtcgc
      541 gggggcgctg cgcgagatgg gggtgtggaa cctgttcacc gggcgccgcg acgacggcgc
      601 gcactacccg ggaggacgcg atttcgccga ggcgccactc ctgccgatcg cggccgatct
      661 gcccgggacg gccggacctc tcgcgcccat cgagccgacc ggcagcctgt cgttgcgcat
      721 cccggccgat cgggcgcgcg gcgctctcag cgtcggcgtg gacggacgcg gcgggcggcc
      781 ggccgccgac ctcctgatct tctaccgctc ggaaggcgcg cagccggtgc tggtgccggt
      841 cgatctctcc gccgggagcg ccaccgttcc aattccctgg actgacgcgc gcgaggcctg
      901 gatcctgctg cgcaaccagg cgaccgcctc cgacgaaggg gcctcgcact tcgacctgag
      961 attcgacctg gacccccgcg tgccgttcga tctggccgcc ttctccgcgg ccgcgctcgg
     1021 gcacggcatc gtgctcgagt ggaccacggc ggcggagcgg ggtcttctgg gctggaacgt
     1081 ctaccgttcc gagtccccgt cgggaccgtt cgtgcggctc aacggcgtcg ccatcccggc
     1141 gtacggcgat ggcaccgccg acaccggcta cgtcttcgcc gacgaagggg tccggcccgg
     1201 tcgacgctac tactaccagg tcgaaggaat cacctcgctc ggtctgcccg agcgaagcca
     1261 caccgccagc gcccgcatcg aagcgcccca ccagtagtca gcaccagccc ttcacgttcg
     1321 cgaggatgtc gtcgcgtccg tcagcgcgac ggcgtggagc gccccgtccc ttgctgcacc
     1381 gtgatcgggc ccggagccgt gatgctgaag tgctcttcgt tcggggcacc gggccagcgc
     1441 acggagaggt ggtacggtgc ggcggccgga tcgaaggcga agaacagccg gcgatccccc
     1501 tgcgaggcgt agctcatgca ggtccgcgcc atgcgcaact gccgcctgcc gccggacgcg
     1561 aggacggcga cggccccgac ggcgtcccgg ttcggggcgc ggccctgaag catgacgacg
     1621 agagagcgtc cggccacggg gccgtcgtgg cgccacagga gggcgggacc gttgttctgc
     1681 gacgcgacga tgtcctcgtc gccgtcgtcg tcgatgtcga acggcacagc accccgcacc
     1741 acgtaccggc ccgcgaaggg gcccgacggc ggcggcaggt cgaaacgtcc gccgccgagg
     1801 ttgcggtaga gctggctcgg catctcgaag gtgatcgtgt cgctatacag cgcgatgttg
     1861 tcgatgatgt ggccgttgcc gacgtagagg tcgagatccc cgtcgaagtc ggcgtcgaag
     1921 aagaaggtcc cgaacccgag catgagcagg gacggaggcc cgagccccga ctcggacgtg
     1981 cggttcgaga aggcgccgtc accgtcgttg cggtacagct cattggtctc ctcggcgagg
     2041 ttggtgacga acaggtcggg gcggccgtcg ccatcgtaat cggccatgtc ggtccccatc
     2101 ccggcctgcg ggatgccgtc ctcggacagc gcacagcccg cgacctgggc gatctcctcg
     2161 aagacgccgc gtccccggtt gcgccagagg aagttcgggg tgtcgtcgtt ggccacgtaa
     2221 aggtcgtcgt ccccgtcgcc gtcgacgtcc cccgcgacca cgcccagccc cttgccgctc
     2281 gtttcgtgaa gtccggcgga ccgggtggcg tccacgaacg tcccgtcgcc gcggttcagg
     2341 tagaagaggt ccggctccgc gtcgaagttc ttgggatgac agacggtgcg gtagccggga
     2401 cggttctcgc cgcagtagag atcctggctc aggttgtagg cgacgtagcg ggcgacgtac
     2461 agatcgagat cgccgtcccg gtcggcgtcg aacagcgtgg cgctcgtgga ccagccgccc
     2521 cccgtcacgc ccgccgccgc ggcgcgctcc tcgaagcgtc ccttcccgag gttcacgtac
     2581 aggcggttgg atccgtagcc ggccacgaag aggtcgcggt cggagtcacc gtcgatatcg
     2641 ccggcgacgc acccgaagcc gtagtgcccg gttgctgcga cgccggcggc cgcggcggct
     2701 tcctcgaagc gcccgccgcc gagattgcga tagaagccgg cgacgggctc cggcgacggg
     2761 gccgcgtccc cggggatcgc gccgccctgc acgaagtaga gatcctgcct cccatccccg
     2821 tcggcgtcga agacgcacac ccccgcaccg agcgtctcga gcatctgctt gcgtccgcgc
     2881 gcgccgttga cgtgccggaa gcggactccc gccccggcgc ccgcgtcgct ccattcgacc
     2941 cagccggagg atcgcggcgc ggagtcggcg gcgcggggcc cggaccccgc cccggcgggc
     3001 tccgggcggc cgcgatcgca gccggcgccg gcggcggaga ggaccgcggc cgcgaggacg
     3061 gcggcggcga cgcgacgcgt cgatctcacg gctgcgctcc cggccgcgga tcgagcagct
     3121 gcatcccgcc gtcgggggtc ctgcggcgca gctcctcgta gcgcgccatc tcggagcgcg
     3181 cccctcccgc gtcgccgcgc gcctgcagga tgccggagag gaagtagtgc ggctcggcga
     3241 actccggctc gatctccgcg gcgcgccgca gcgccgtcac agcttcgtcg agtcggttct
     3301 cgagcgccag gagacgtccc atctcgagcc aggcccgctc cccgcgcggg tcgagctgga
     3361 ggctccgatc gagggcccgg cgcgcctcgt cgcggcggtc gagcgccagg agcgaggacc
     3421 cgatgaacag ctgcgcctgc ggatccgcgg gatcctcgcg ggcgatgatg cggaactcct
     3481 ccagggcacg gccgtactcc tggcggttga acgcctccac cccggcgatg cgatgactgc
     3541 gcacccgctc gtcgacccgg cggcgatcga gcgccgcctg gtgccgcgcc ccggcctgcg
     3601 ccgccttctc ggcgcgcccc agccgcgccg ccagcgtgcg cacgtggctg agcgccccga
     3661 ggtgccccgg atcgatctcc gcggcccgtt cgaaggccgc aagcgcctcc tcgcgctccc
     3721 ccgccgcctc gagaccgagg ccgatgaggt actgcgcctc cgcggagcgc ggctcccgct
     3781 ccgccgcccg ccgcgcggcc ttcagcccgc cggcggaatc ccccgcccgc aggcgcagcc
     3841 gggcctcctg gagcaggacc gggggcgcgt cgccgcgatc cgtgaggagc ggcgccagga
     3901 cctcgagccc ctcggcgttg cgccccatgt ctccgaggag acgcgccagc cccagccgcg
     3961 cgccgggcga ggccgggtcc gcctcgagcg cctgccggta ggaggtctcc gcctggccga
     4021 accgatcgct ccgggcgtac acctggccca ccgcctcgag caggcgcgca tcgcggggcc
     4081 gcgcggcgag ggcgcgcacg aggagcggct ccgcggcgtc gacggcaccg ctctggagga
     4141 ggcgcatccc ttcgcgcgcc gcggcgtcgg cctccggagc ggcggcgggc gcggccggcc
     4201 cctgcggctc gggacgctcc ccgctcggac aggcggcgag gaggatcgcg agcgcggcgc
     4261 agccggcggg gacgcggcca gcgccgcgaa gggagatcac gcggggatct tacagaactc
     4321 ggtgcttccg ccgccggaaa cggccttccg ggtgcgtgat agactcgccg gccggaggca
     4381 cggtggaggg gttcaaggac gagtgcgccg cggcgatccg cgagcaggtg gcgagcctct
     4441 acggcgcggg gcccgagaag atcgtcgtcg aatggccgcc gcagcccggc atgggggatc
     4501 tcgcctcccc cgtccccttc gagctcgccc gccggctgaa gcgcccgccg cgcgccatcg
     4561 cgcaggagct gtgcgaccgt ttcccgcggc tcccctggct gaagcgggtc gaagtcggag
     4621 gggccggata cctcaacgtc ttcgtcgatc gcggcatggc gctgcgcgcc ctcgcccgcg
     4681 acctgcacgc cccggaggcg cggccgtcgg gggaccggat catcgtcgag cacaccaaca
     4741 tcaacccgaa caaggccgcg cacatcggcc acctgcgcaa cgcggtgctc ggcgacagcc
     4801 tcgcccggag cctgcgcttc ctcggccacg aggtcgaagt gcagaactac atcgacgaca
     4861 ccggcgtgca ggtggccgac gtcctcatcg gcttccggca catcctcggc atggggctcg
     4921 aagcggcgca ggcgatcccg gatccgatcg accgtttctg ctgggacctc tacgcgaagg
     4981 tcggcgagtg gtacgaggcg gatccgaagc ggcgggccct gcaggcggag acgctcaaga
     5041 gcctggagcg gcgcgagggg gagatcggcc gcttcgccgc ctggctgacg cgcgccatcg
     5101 tctcccggca tctcgacacc atggaccgcc tgcacgtgcg ctacgacctc ctcccgtggg
     5161 agggggacat cctggcgtac cgcctctggg agaccgcctt cgcgctcctc aaggagaaga
     5221 aggcgatcgt gagggtcgag tccggggaac gggccggctg ctgggtgatg gcgctcgacg
     5281 aggagcgttt ccccgatctc aaggaggcgg agaagatcct cgtgcgcagc aacggcacgg
     5341 tcacctacgt cggcaaggac atcgcctacc agatgtggaa gttcgggctc ctgccgagcg
     5401 atttccgcta cgaggtgttc cggcgcgagc cggacggaca cgcgatctgg agctcgacga
     5461 gcggccccgg cgatcccgcg gcgccccgct tcgggcgcgc ccgccgggtg gtgaacgtca
     5521 tcgacgtgcg gcagtcgtac ctgcaggcga ttgtggctga gggcctgaag gccctcggcc
     5581 acaaggagga ggcggagcgc tcggtgcact tctcctacga gatggtggcg ctcaccccgc
     5641 agagcgccga gcgcctcggc gtgccgctgt cggaggagga tcgcggccgt ccctacgtcg
     5701 gcatgtcggg acggaaaggg ctgggcatca aggcggacga tctgatcgac gcgcttcagg
     5761 cgcaggcgct caaggaggtg cgcgcccgcc acgccgatct cgaggaggcg gaggccgggg
     5821 ccctggcgcg cgccatcgcc gtcggagcgc tgcgctactt catgatcaag ttcacgcgca
     5881 acaaggtgct ggccttcgac ttcgacgacg cgctgtcgtt cgagggggag accggcccct
     5941 atctgcagta cgccgcggtg cgggcgtccg gcatcttcga gcgcatgtcc gcctcgggcg
     6001 gccccggaga gacggaggcg ggccggctcg cgatcgaagc ggccttcgac gtccccgagg
     6061 gccccgaggc ggaggaacac tgggcgctcg cccacctcat cgcccggttc cgggaggcgg
     6121 cgtcccaggc ggccgcgagc ctcgagctgt cgcttctcgc caagtttgcg ttcggcctgg
     6181 cgcagcggtt caacaccttc tatcaccgct tcccggtgat gaaggagccg gatccaagct
     6241 ggcgggccgc ccgcgtcacc ctcacctggc tgttcctggc gcagatgcga cgcgcctgtt
     6301 ccctgatggg gatcccgatc ccggacagga tgtgagatga cccgacacgt gggtgcgccg
     6361 cggcggctcg cggcgctcgt cgtcctcctg atcgcgacgc ccgccgctgc gctcggccag
     6421 gagatcctcc ctttcagcga agtcaaggcc ggcatgtcgg gcgtcggcct gaccgtcttc
     6481 tccggcaccc gcatcgacga gttcaaggtc gaggtgatcg gaaccctcga gaacatcgcg
     6541 ccgcgccgga acctcatcct gacgcggctc tccgggggtc ccctgcagga gaccggcgtc
     6601 ctgagcggca tgagcggcag cccggtctat ttcaacggac gactcgccgg ggccgtcgcc
     6661 tacacctggg ggttcgcgcg cgagccgatc gccggcgtcg tcccgatcca ggagatgctg
     6721 tcgatcgaga cgcgcgagcc gcccgcgacg cgcacctccc gcctgccggc cggcgcgggt
     6781 ccggcggggc tgaagtggct caccgatccg gcggcgatcc cggcccattt cgcctcctgg
     6841 ttcgatcgcc tgccgggagg gccggcgccg gaagggctgg cgccgatcgg cgctccgctc
     6901 ctggtgcgcg gcgtgccggg ggacgtcgtc gatctcctgc gtccggggct gcggcgcgcc
     6961 gggctgctgc cggtgcaggg gggttcgatc tcgaaatcgg agaagggggc cgatgcggcc
     7021 ggcctgctcc cggggagcgg cgtcgggatc gccctcgtcc agggggacgt ggagatcgcc
     7081 gcggtctgca ccgtgacgca tcgcgacgcg gatcggctct acgcgtgcgg tcacccgctc
     7141 ctgaatctcg gccccaccga gctcgcgatg accaccgcga gcgtcagcgg cctgatgccg
     7201 tcgctcgaat cgtccttcaa gttcgcgagc tccggcggcg aggtgggggt gttccgccag
     7261 gaccgctcca ccggcgtgtt cggatacgtc ggaaagaagg cccggacgat cccggtgcgg
     7321 ctggatctcc agccggagcg cggccgcccg aaacgcatgg cgttcgaagt ggcggacgat
     7381 cccttcctgg ccccctacct cctctacgcc gccctgaacg cggtgttcag cagcgaggag
     7441 aaggactacg gcaccgtgtc gttcacctac aaggaggggt cgcagatccg cgtcgagggg
     7501 gaggaggaga tcgtgctccg gaacctgttc gccggagacc tggcgcgcct gtacgcgtcc
     7561 gggacggtgg ccttcctgac gcaggtgctc ctcgacagcg agtaccggag cgtccggatc
     7621 gccggcatcg atctcgtcct cggcacctcg gacgagcggc gcaccgcgcg gctggagcgc
     7681 gcctgggtca gccgcgatcg cgtccggccg ggcgagacga tcgaggtgtc gatcgggctg
     7741 aagccgttcc gcggcccgga gctgacgcgg gagatgcggt tgaccgtccc gcccgaggtc
     7801 ccgcccgggc gcctgctcct gcaggtgggg gacgcctcgg cgctggcgcg caccgatccc
     7861 gaggcggggg acgacttcgt gccgcgcgac ctgcgccagc tcgtgtggct catcaaccac
     7921 ctgcgccgca acgaccgcct ctacgccgtc ctcgtccgtg ccgacaacgg gctcgtgttc
     7981 cagggggagc gcctgccgaa cctgccgccg tcggtggcgc aggtgatggc gcgcccggac
     8041 acgcggggca acgtgctccg ggtgcagtac cgcggcgtcg cggaggagtc gctgtcgctc
     8101 ccctacgcca tcgacggata ccgcctcatc gccatcgacg tggaggaatg acgtgagccg
     8161 atccctgcga tctccctgcg ccgcggcgct cctgtggctc ctcgccggtg cggcgcccgc
     8221 ccgcgcggtg tcgccggtcc tctggaccac cgaaaccctc gacgacttcg agaaggggaa
     8281 gccggacggc atcgccctcg gggcgccggg ggagctgacc ctgagcccgg cgcttctggg
     8341 tctgaacgtc ccggcgctcg agcagtcgag cgagccgttc ctctggtcgg tggcggtcga
     8401 ttcgaagggg acggtgtacg cggggggcgg ccggaacggc accgtctacc gcgtgccgaa
     8461 gggaggcgcc ggatccgcct ggttcgagag cggcgcgctg gcggtgcacg cgatcgtcgt
     8521 cgacaagagc gacgcggtct tcgccgccac ccttccggag gggcgggtcg ttcggattac
     8581 cggcgagggg aagggggaga cctggtacca gccggaggat cgctacatct gggacctcgc
     8641 gctggggccg aagggggagt tgtacgccgc caccggcgag cgcggcatca tctaccgcat
     8701 caccggcttg ggcaaagcgg aggtgttctt cgacagtccc gagttccaca tcgtgtcgct
     8761 cgcggtggac gcccagggga acgtcctcgc cggatcggac ggcaagggac tcctgtaccg
     8821 gatcacgccg caggggaagg cgtccgtgct ctacgactcc ccgctccggg agatcgccgc
     8881 cgtggcggtc gatccgaagg gggcgatcta cgctgcggcg ctcggcaccg aaggggaggc
     8941 tccgacgctc cccctcctga tcccgcagac gatgccgatc gggcgcgagc aggttcccgg
     9001 gctccccggc cagccgccgg tgcaggtgcc ggggatcgcc gaggagccgt cggcgcgcgt
     9061 caccgtgacg accgtcagcg ccagcctcgt cccgaccggc cctccaccgc gcagtgaagt
     9121 gtaccgggtc gatccggacg ggacggtggt cacgctgtgg tcctcgccga ccgaggtggc
     9181 gtacgccctg tcgatcgacg ccgccgggcg gccggtgct
//
LOCUS       DASRIQ010000625        10752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1562346, whole genome shotgun sequence.
ACCESSION   DASRIQ010000625 DASRIQ010000000
VERSION     DASRIQ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562346"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1596
                     /locus_tag="VFD06_15535"
     CDS             103..1596
                     /locus_tag="VFD06_15535"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN04996.1"
                     /translation="MLPSRTTRRRLLAILAAAGVFAAILGLSATRVGAEGETCPKCGA
                     ARKAGAAFCVACGQKFATAAPAAAAAPRPSVVQVVAAHDNELTSTYGSLVWGSNLRVD
                     SLVGSAFAVGRGEFVTDSGLMVGARDVKLRTPSGRTVPAQVIGTDPMIGITLLKAEVD
                     EVTPLELRRDAPAKIGEGMTALGYPSGSQARGEPVVSQGVVSGLHRGGARIHPVEDYL
                     QSDASLPRGLAGGPMLDGGGRVIGMSTAFVMGGRVFLGLQSGIGFAVPADWIDRGLAW
                     IRAGSPPRGWVGALAVPADSERRERYGLPPEVRLVVEHVFPGSPAAAAGVKRGDGLVE
                     LGGESISTLPRAQEQLLAARPGDTVSLSVVRTGGPVRLDVPLAPRPDRPRLSAIDALR
                     FFAELEVVARGEDLVVGPVRSGSTPAYYKVASGDVLKSVLSKKDWEHGATDNSRWRSV
                     KTVADLEERLATAYSDFDFTVGLRFKSKDGAKREMYVWEILTPTASL"
     gene            complement(1719..2282)
                     /locus_tag="VFD06_15540"
     CDS             complement(1719..2282)
                     /locus_tag="VFD06_15540"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZN04997.1"
                     /translation="MIAEQTRTIAKVGSPAPDFAMESTKNLEKLDQPVKLSDYRGKWL
                     VMFFYPLDFTFVCPTEIVAFSDANERFRAAGAEVLGVSIDSQFVHRAWIKTPRNQNGL
                     GELQFPLAADLTKSVSRDYGVLLEDKGIALRGLFIIDPKGVVQYAVTHNLNVGRSVDE
                     TLRVLQALQTGERCPANWKPGSATLKV"
     gene            complement(2279..2776)
                     /locus_tag="VFD06_15545"
     CDS             complement(2279..2776)
                     /locus_tag="VFD06_15545"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZN04998.1"
                     /translation="MPVPRLKDLETALRRRGARPTRQRRVVYAALAERCDHPTAEAIH
                     RAARRRSPGMSLATVYTTLDLLVEAGLAARVAGPDGAARFDARTDRHDHRRCLACGRI
                     EDVERSGEGPRLDPYQRPDFHAVDLRLEIVGYCSGCQDAAGASHGTAIPAGPPSNDHT
                     QGDSR"
     gene            complement(2840..3607)
                     /locus_tag="VFD06_15550"
     CDS             complement(2840..3607)
                     /locus_tag="VFD06_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HZN04999.1"
                     /translation="MKNTPLLEALLSVMDRKDHWAYPHFSEGRATRPQLLLHYQQEHA
                     VYVRDFPILLSRVHARCPHAEVRQDLAENLYEEETGGLSKSKPHPELFLEMMQGLGFT
                     GRQFERIDLVPEAAAYRAFVDQVTTRRPWIEGALVVTIFIEGSRADRREVTGNEAAHP
                     ADPEPGLKEHFLVRHYGVDPRWLDLKRAHHKVESGHRRMAWKMVLDHARGDGAGGRLT
                     RLLEQTLALWLLYRDGVARASGIPFPFSSKSKLHKIK"
     gene            complement(3672..4706)
                     /locus_tag="VFD06_15555"
     CDS             complement(3672..4706)
                     /locus_tag="VFD06_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05000.1"
                     /translation="MTPRPVLLRPFEGAAILALALLACAAACAAFGAPRAAAGAASSP
                     AAARPTRGDLEAARRRAAVIESELALASTRKPYLVLDLGARALRYRMMGLTVREVPLL
                     DVDVEGLQTAELASGAPLIAGIFTLKEKEGDPRLTPLSPEQVEEGADDENTANALPPE
                     PPREFTLAFKQPIAVEIVGRETRSGVQGMWGRMIGALRRWHGPGGGEETRLRIALHLD
                     PEIAAEVYRSLIPDLRLLVLPPDGMQLPAAGQEAPPKPRAARKTPPPREKEPEPKEVP
                     FKIPPPEDVNGQRPADETQETGEPGSGEPGPAGEETAPPAPSPAPTPPSDPPPPPERR
                     PGRTDRGPST"
     gene            complement(4706..5347)
                     /locus_tag="VFD06_15560"
     CDS             complement(4706..5347)
                     /locus_tag="VFD06_15560"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZN05001.1"
                     /translation="MRRREVIVAAVALAAVLALVLFFLQHRGPAPAAADARPLDAAGQ
                     KELRSLQARTDGLERRLAGLKPKAAIYVVIDTGRNRLSLYENGAETLKAVCSTGSGRA
                     LVDPERKRRWVFDTPRGEFFIRAKHPDPVWIKPDWAFLEVGEAIPRRLAERLAPTELG
                     AYAMDLGDGYLIHGTLYKRALGLSVTHGCVRLGDDDLDQIYHKVRIGTPVYIY"
     gene            complement(5344..6396)
                     /locus_tag="VFD06_15565"
     CDS             complement(5344..6396)
                     /locus_tag="VFD06_15565"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZN05002.1"
                     /translation="MAAVAILLAACGQPPAAQIEAARKALKRAAASGGVLRAPEICAA
                     AQAALTRAEAEIRVQAKKASWSRRYRFAERLAVVALDTGRSCAAHAEAVQEGRKRRAS
                     GSLGDLQAAIDHASALARHTSDPDIQAFLMRATLSLGEGRSSFDKGEYERASAAAGRG
                     RAQVATAVHEIDRFIDDYRKSPRREVWRKWLRGTLEQSRDEERTVLVVDKLRRELLVV
                     RGDDELASFRVDLGLGGMEHKTRAGDAFTPEGRYKVTEVRNPGQTRYYRALMLDYPND
                     EDRRRFKSLQKKGLLPRRGGIGSNIEIHGEGGREQDWTQGCVALSNDDMDALVRYVQV
                     GTPVTIVGTIPDGEYE"
     gene            complement(6486..7082)
                     /locus_tag="VFD06_15570"
     CDS             complement(6486..7082)
                     /locus_tag="VFD06_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05003.1"
                     /translation="MKRVTLLTLAVLVLFAVAGCAKPPQDEKAAAQAEMDRAQQANAD
                     TWAPTEAKAAQEAMTAADAEISAQNEKWMKNYDKAKELLAQAKDNAAKAAEAAATNKA
                     QAKTDSEAAIAAADTAIQTAEAALKTAPKTKDSAADLKLMENDLTTLKSTLESAKAAA
                     AAEDYKKALESANSVKDQATAMSTQIEEAKAKRAGVKK"
     gene            7289..8110
                     /gene="proC"
                     /locus_tag="VFD06_15575"
     CDS             7289..8110
                     /gene="proC"
                     /locus_tag="VFD06_15575"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZN05004.1"
                     /translation="MARAAVERIAVLGAGKIGECIIGGLLESSRLDPRTLTATARHAE
                     RLEHLSTRYKVATTLSNRAAVRGASLVLLAVKPQAMEEVLRDIRPVVRRGQLVLSIAA
                     SVPTGFIEKRLPAGVAVVRAMPNTPCVVGQGMTVLSGGRNARAEHLEAARRIFAPLGL
                     CLALDEKHMDAVTALSASGPAYLYVVIESLAEGGVKVGLPREVATTLVAQMVLGSATM
                     VLKSGEHPATLKDAVTTPAGCTIDGLLALEEGGLRVALIRAVVRATRRAGELLHG"
     gene            8126..8608
                     /locus_tag="VFD06_15580"
     CDS             8126..8608
                     /locus_tag="VFD06_15580"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZN05005.1"
                     /translation="MSGLPNLHPAIVHLPLALLPAAILFDLTALVRPTWTWLERGGSL
                     LLILAALGCAAAVLAGEQAEEGIGPQPQHIRDLLDEHEDFGKKSLLATSALAVARLLL
                     SIRDRDRPRLTALFLRSLLLVAACGALFLMAQAADRGGALVYRHGLAVERAPVPPGDL
                     "
     gene            complement(8622..10310)
                     /locus_tag="VFD06_15585"
     CDS             complement(8622..10310)
                     /locus_tag="VFD06_15585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN05006.1"
                     /translation="MKTRGLAKTSGFFACLLLALYAWQAAPGIARMAPTFDEPAHVGA
                     GMSYLETGDFKLNLQHPPLLKEIAAVPLLWGGIRWPVSERNWIESGPDPGPLMQWEVG
                     SSVLYLNDPDRVMLLARAPFLLLTLLLGWSVFAWGRQMLGPFAGLAAVGLLALDPSMV
                     AHGVLVTTDAGFALFAFLSVWTLWRWLQHRTMPRLLLCGLALGAALASKFTGVLLLPV
                     LLVFFLAAARWIPASVQHRPSTLFDPYAGEAMTPRVVWCLYSFAGLVVVAGLVIWASY
                     FFHSPALYWTGLLRVNADHDPSYWPYMGGTFRPHFWSYYLVAWLLKEPLPAILLVATG
                     VWALMFRGGVTAMDRAFLLVPPAGLFFFYTFLSHNLGFRYLIPALPFLALVGGVGAKA
                     LWESKAIAGRAGLAVLGVWLGWNAWGIAPDHLAFFNESACLTTAPSRLGRDGGTACGP
                     LWLDDSNVDWGQGLKTLAAWLRQNAPGRRIGLAYFGTARPDYYGIGYDRLGTDELMRG
                     LPMGLYAVSTHLAARTRGHLAVKYGDGPQNWVEHQKPRAVVAHAYYIYDIPRPP"
     gene            10382..>10752
                     /locus_tag="VFD06_15590"
     CDS             10382..>10752
                     /locus_tag="VFD06_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05007.1"
                     /translation="MNGATSRDEAAPAGDAATAPPLAGPDAFLERRSGPLAFALLALL
                     VLQGIAFIAESSQTSDEAAHLSAGYSYLTRGDFRLNPEHPPLIKELAALPLLPLGLDF
                     PEGPLWQMAEEWNIGRIFVHE"
BASE COUNT         1509 a         3805 c         3817 g         1621 t
ORIGIN      
        1 cctacgtcat caggatcgag atgcactcgg agggggagga caaggagatg cgcaattatc
       61 tgaacctctt ccccgacctc ctcgcgccgc cggtctactg aaatgcttcc atcgcggacg
      121 acccgacgcc ggctcctcgc gatcctcgcc gccgcggggg tgttcgcggc gatcctcgga
      181 ctgtcggcca cccgggtcgg ggccgaagga gagacctgcc cgaagtgcgg cgctgcgcgc
      241 aaggcgggag ccgccttctg cgtcgcctgc ggccagaagt tcgccacggc ggcgccggcc
      301 gcggcggccg cgccgcgccc gtccgtcgtc caggtggtgg cggcgcacga caacgagctg
      361 acctcgacct acggctccct cgtgtggggt tccaacctga gagtggactc cctcgtcggt
      421 agcgcgttcg ccgtcggacg cggcgagttc gtcaccgaca gcggcctgat ggtgggcgcg
      481 cgcgacgtga aactgcgcac gccctccggc cgcaccgttc cggcgcaggt catcggcacc
      541 gatccgatga tcggaatcac cctgctcaag gcggaggtgg acgaggtcac accgctggag
      601 ctgcgccggg acgcgccggc gaagatcggc gaggggatga ccgccctcgg atatccgagc
      661 ggcagccagg cgcgcggcga gccggtcgtg tcgcagggag tggtcagcgg gctgcaccgc
      721 ggcggcgccc gcatccaccc ggtcgaggat tacctccagt ccgacgcctc cctgccgcgc
      781 gggctcgccg gggggccgat gctggacggc ggcggccggg tgatcggcat gagcacggcg
      841 ttcgtgatgg gagggcgggt gttcctcgga ctgcagagcg gcatcggctt cgccgtcccg
      901 gccgattgga tcgatcgcgg gctcgcgtgg atccgcgcgg gatcgccgcc gcggggatgg
      961 gtcggcgcgc tggcggtgcc ggccgactcg gagcgccgcg agcggtacgg cctcccgccg
     1021 gaggtgcgcc tcgtcgtcga gcacgtcttc cccgggagcc ccgccgccgc cgcgggggtg
     1081 aagcggggcg acgggctggt cgagctcggg ggagaatcga tctccacgtt gccccgggcg
     1141 caggagcagc tgctggcggc gcgtcccggc gacacggtgt cgctgtcggt cgtgcggacc
     1201 ggagggccgg tccggctcga tgtcccgctg gctccccgcc cggaccgtcc gcgcctgtcg
     1261 gcgatcgacg cgctgcggtt cttcgctgag ctggaggtgg tggcacgcgg cgaggacctg
     1321 gtggtcgggc cggtccgctc aggaagcacc ccggcctatt acaaggtggc ctccggcgac
     1381 gttctcaaga gcgtcctcag caagaaggac tgggagcacg gcgccaccga caactcgcgc
     1441 tggcgctcgg tcaagaccgt tgcggacctg gaggaacggc tcgcgaccgc ctactccgac
     1501 ttcgatttca cggtcgggct ccgcttcaag tccaaggacg gcgcgaagcg cgagatgtac
     1561 gtctgggaga tcctcacgcc gacggcgtcg ctgtgagcgg ggcgaccggg tcgcgctccc
     1621 cggccgcccc gcacgcgagc cgcgtgtcct agcgggatcc ccgcgcgtcc accggcgccg
     1681 caccggcttg gccgccatga tgcgtgctcc ttccccggct agaccttcag ggtcgcgctg
     1741 cccggcttcc agttggcggg gcagcgctcg ccggtctgca gcgcctgcag aacgcgcagg
     1801 gtctcgtcga cgctccggcc gacgttcagg ttgtgggtga cggcgtactg caccaccccc
     1861 ttcgggtcga tgatgaacag gccccggagc gcgatcccct tgtcctcgag cagcacgccg
     1921 tagtcgcggc tgaccgactt cgtgagatcg gcggccagcg ggaactgcag ctcgccgagg
     1981 ccgttctggt tgcgcggcgt cttgatccac gcccggtgca cgaactggga gtcgatgctc
     2041 accccgagga cttcggcgcc ggcggcacgg aagcgctcgt tcgcgtcgga gaaggcgacg
     2101 atctcggtcg ggcagacaaa ggtgaagtcg agcgggtaga agaacatcac cagccacttg
     2161 ccgcggtaat cggacagctt caccggctgg tcgagcttct cgaggttctt ggtggactcc
     2221 atggcgaagt cgggagcggg gctcccgacc ttggcgatgg tccgggtctg ttcggcaatc
     2281 atcgtgagtc tccttgcgtg tgatcgttgg atggcgggcc cgcggggatc gcggtcccgt
     2341 gggacgcccc ggccgcgtcc tggcagccgg agcagtagcc gacgatctcg agtcgcagat
     2401 cgacggcgtg gaaatcgggt ctctggtacg ggtcgaggcg gggcccctcc cccgaccgct
     2461 cgacgtcctc gatccggccg caggcgagac agcggcggtg atcgtgacgg tcggtgcggg
     2521 catcgaagcg cgccgccccg tcggggccgg cgacgcgggc ggcgaggccg gcctccacca
     2581 ggagatcgag cgtggtgtag acggtcgcga gcgacattcc cggcgagcgc cggcgcgccg
     2641 cacggtggat cgcctccgcg gtcggatggt cgcagcgctc ggcgagggcg gcgtacacga
     2701 cgcggcgctg gcgcgtcggc cgcgcgccgc gacgccgaag cgccgtctcg aggtccttca
     2761 gtcggggaac gggcatgacg cggcgcagtc tagcccgggg cgccgcaggc gtcaatacta
     2821 tttcttgtta accaaataac tattttattt tgtgtagttt gcttttagag ctgaagggaa
     2881 acgggatgcc cgaggcccgg gcgacgccgt cgcggtacag cagccacagg gccagcgtct
     2941 gctccagaag acgcgtgagc ctcccgccag ccccgtctcc ccgcgcatga tccagcacca
     3001 tcttccaggc catgcgccga tggccgctct ccaccttgtg gtgcgcccgc ttcagatcga
     3061 gccagcgcgg atcgacgccg tagtggcgca ccaggaagtg ctccttgagc cctggctccg
     3121 ggtcggccgg gtgcgccgct tcgttccccg tcacctcgcg gcgatccgca cggctcccct
     3181 cgatgaagat ggtgacgacc agggccccct cgatccacgg gcgccgggtg gtgacctgat
     3241 cgacgaaggc gcggtaggcc gccgcctcgg ggacgaggtc gatccgctcg aactgccgcc
     3301 ccgtgaagcc gagcccctgc atcatctcga ggaacagctc gggatgcggc ttgctcttcg
     3361 agagcccgcc ggtctcctcc tcgtagaggt tctcggccag gtcctggcgc acctccgcgt
     3421 gggggcagcg cgcgtgcacc cggctcagaa ggatcgggaa gtcgcgcacg tacacggcgt
     3481 gctcctgctg gtagtgcagg agcagctggg gacgcgtggc gcgcccctcg ctgaagtgcg
     3541 ggtacgccca atgatccttg cggtccatga ccgacagaag cgcttcgagc agcggtgtgt
     3601 ttttcatggc ctctcgacgc tccccgagcg gggaacggac cctctcaaga tggcatcccc
     3661 cgggcgcggg atcaagtcga tggaccccga tccgtccgtc ccgggcgcct ctccggcggc
     3721 ggaggcggat ccgagggcgg cgtcggggcg ggagagggtg cgggtggcgc cgtttcctcg
     3781 cctgccggcc ccggctctcc cgaccccggc tccccggtct cctgcgtctc gtccgcgggc
     3841 ctctggccgt tcacatcctc cggcggcggg atcttgaacg gcacctcctt cggctccggc
     3901 tccttttcgc gcggcggcgg ggtcttgcgc gcggcgcgcg gtttcggcgg cgcctcctgt
     3961 cccgcggccg ggagctgcat cccgtccggc ggcagcacca gcagccgcag atcggggatg
     4021 agggagcggt acacctccgc ggcgatctcg gggtcgaggt gcagggcgat gcggagacgc
     4081 gtctcctctc cgccccccgg accgtgccag cgccgcagcg cgccgatcat cctcccccac
     4141 atgccctgga cgccgctgcg ggtctcccgc cccacgatct ccacggcgat cggctgcttg
     4201 aaggcgagtg tgaattcgcg cggcggttcc ggcggcaggg cgttggcggt gttctcgtcg
     4261 tccgccccct cctccacctg ctccggcgag agcggcgtca gccgcggatc gccttccttc
     4321 tccttgagcg tgaagatgcc ggcgatgagg ggcgcccccg acgccagctc cgccgtctgc
     4381 agcccctcga cgtccacgtc gaggagcggc acctcgcgca ccgtcagccc catcatccgg
     4441 tagcgcagcg cccgggcgcc gaggtcgagc accaggtacg gtttgcgggt ggaggccagg
     4501 gcgagctcgc tctcgatgac cgcggccctt cggcgggccg cctcgaggtc gccgcgcgtt
     4561 ggccgggcgg cggccggcga cgacgcggcc ccggcggcgg cccgcggcgc accgaaagcg
     4621 gcgcaagcgg cggcgcaggc gaggagcgcc agcgccagga tcgcagcgcc ttcgaaggga
     4681 cggaggagaa cgggtcgcgg cgtcatcagt agatgtacac cggcgtgcca atgcgcacct
     4741 tgtggtagat ctggtcgaga tcgtcgtccc cgagccgcac gcagccgtgc gtgacgctca
     4801 ggccgagggc gcgcttgtag agcgtgccgt ggatcaggta cccgtcgccg aggtccatcg
     4861 cgtaggcccc gagctcggtc ggggcgaggc gctccgccag gcggcgcggg atcgcctctc
     4921 ccacctcgag gaacgcccag tccggcttga tccagaccgg gtccgggtgc ttggcgcgga
     4981 tgaagaactc accgcgcggc gtgtcgaaga cccagcggcg cttgcgctcc gggtccacga
     5041 gcgcgcggcc cgagccggtg gagcagaccg ccttcagcgt ctcggcgccg ttctcgtaaa
     5101 gcgacaggcg gttgcggccg gtgtcgatca cgacgtagat cgcggccttg ggcttgagcc
     5161 cggcgagccg gcgctcgagg ccgtcggtgc gcgcctgaag cgaccgaagc tccttctgcc
     5221 ccgcggcgtc gagcgggcgc gcgtcggccg ccgccggggc cgggccgcgg tgctggagga
     5281 agaacagcac gagggcgagc accgcggcca gggcgacagc cgcgacgatg acttcgcgtc
     5341 gcctcattcg tactccccgt ccgggatggt tccgacgatg gtcaccggcg tccccacctg
     5401 cacgtacctc acgagcgcgt ccatgtcgtc attggagagg gcgacacaac cctgcgtcca
     5461 gtcctgctcc cgtcctcctt caccgtggat ctcgatgttg ctgccgatgc ccccgcgccg
     5521 cggcaggagc cccttcttct ggaggctctt gaagcggcgc cggtcctcgt cgttggggta
     5581 gtcgagcatc agcgcccggt agtagcgcgt ctgccccggg ttccgcacct cggtgacctt
     5641 gtagcgtccc tcgggcgtga aggcgtcgcc ggcgcgcgtc ttgtgctcca tgccgccgag
     5701 gccgagatcc acccggaacg acgccagctc gtcgtcgccg cgcacgacca ggagctcccg
     5761 ccgcagcttg tcgaccacga gcacggtgcg ctcctcgtcg cgcgactgct cgagcgtgcc
     5821 gcgcagccac ttccgccaca cctcgcggcg cgggctcttc cggtagtcgt cgatgaaccg
     5881 gtcgatctcg tgcaccgcgg tcgccacctg cgcgcgtccc cgtcccgccg ccgccgaagc
     5941 gcgctcgtac tcccccttgt cgaaggagga acgtccctca cccagggaga gagtcgccct
     6001 catcaagaag gcttggatgt cgggatccga agtgtgacgc gccagggccg aggcgtggtc
     6061 gatcgccgcc tggaggtcgc cgagggagcc gctggcgcgc cgcttgcggc cctcctgcac
     6121 cgcctcggcg tgcgccgcgc acgaccggcc ggtgtcgagt gcgacgaccg cgagccgctc
     6181 cgcgaagcgg taccgcctcg accaggaggc cttcttcgcc tgcacccgga tctccgcctc
     6241 cgctcgcgtc agcgccgcct gggcggcggc gcagatctcc ggcgcccgca gcacgcctcc
     6301 cgaagcggcg gcccgcttca gcgccttcct ggccgcctcg atctgcgccg cgggcggctg
     6361 tccacacgcc gcgaggagga tcgccaccgc cgccacggcg ggcagccgca ggcggcccga
     6421 agacgagaag ggcccggctg gtgcgcgacc ggccgggccc cgaacctctg ccaacgtggc
     6481 ggtggctact ttttcactcc cgcccgcttc gccttcgcct cttcgatctg cgtcgacatc
     6541 gcggtggcct ggtccttcac cgagttggcg ctctccagcg ccttcttgta gtcctcggcc
     6601 gcagccgccg ccttcgccga ctcgagcgtc gacttcagcg tcgtcaggtc gttctccatc
     6661 agcttgagat cggccgcgga gtccttggtc ttcggtgcgg tcttgagcgc cgcctcggcg
     6721 gtctggatgg cggtgtcggc cgccgcgatc gccgcctcgg agtcggtctt cgcctgcgcc
     6781 ttgttggtcg ccgccgcctc ggccgctttc gccgcgttgt ccttcgcctg ggcgagcagc
     6841 tccttcgcct tgtcgtagtt cttcatccac ttctcgttct gagcgctgat ctcggcatct
     6901 gccgcggtca tcgcctcttg ggccgccttg gcctcggtcg gggcccaagt gtcggcgttc
     6961 gcctgctggg cgcggtccat ctccgcctgt gcggcggcct tctcgtcctg aggcggcttg
     7021 gcgcagcctg ccaccgcgaa caggaccagg acagccaggg taagaagcgt gacacgtttc
     7081 atgatcaggg ctcgcctcct cgcagaagtt attgaacgga tggagcgttt tcgacgtcgg
     7141 caccggccgc gatcatcacc cctggtgcgg cccgatgccc atgtccggac cccaggaggg
     7201 gcgggaggat agcagatcgg gatgaggctg ccaacagccc cccagaccgc ggaaaagatt
     7261 agacttccgc ggcggcggag gggtccggat ggcgcgcgcg gcggtggagc ggatcgcggt
     7321 tctcggggcc gggaagatcg gcgagtgcat catcgggggt ctgctcgaat cgagccgtct
     7381 cgacccccgg acactgaccg ccaccgcccg tcacgccgaa cggctcgagc acctcagcac
     7441 ccggtacaag gtggcgacca ccctgtcgaa ccgggcggcc gtgcgcggcg cctcgctcgt
     7501 cctcctggcg gtgaagcctc aggccatgga ggaggtgctg cgcgacatcc gcccggtggt
     7561 ccgccgcggc cagctcgtcc tgtccatcgc cgcgtcggtc ccgaccggat tcatcgagaa
     7621 gcggctcccg gcgggggtgg cggtggtccg cgccatgccg aacaccccct gcgtcgtcgg
     7681 ccaggggatg accgtccttt cggggggccg gaacgcgcgc gcggagcatc tcgaggcggc
     7741 ccggcgcatc ttcgcgcccc tcggtctctg cctggcgctc gacgagaagc acatggacgc
     7801 ggtcacggcg ctgtcggcgt cgggaccggc ctacctgtac gtggtcatcg aatcgctcgc
     7861 cgaaggaggg gtgaaggtcg gcctgccgcg cgaagtggcc accaccctgg tcgcgcagat
     7921 ggtcctcggg tccgccacga tggtgctgaa gtcgggagaa cacccggcca cgctcaagga
     7981 cgccgtgacc accccggccg gctgcaccat cgacggactc ctggcgctcg aggagggagg
     8041 attgcgagtg gcgctcatcc gggcggtcgt ccgtgcgacc cgccgcgcgg gagagctcct
     8101 gcatggctga ccaggagtga ccggcgtgag cgggctgccg aacctgcacc cggcgatcgt
     8161 ccacctgccg ctggcgctgc tgccggccgc gatccttttc gacctcacgg cgctcgtgcg
     8221 accgacctgg acctggctcg agcgcggcgg gtcgctcctg ctgatcctgg cggcgctcgg
     8281 atgcgccgcg gcggtcctcg ccggggaaca ggcggaggag gggatcggcc cgcagccgca
     8341 gcacatccgc gacctgctgg acgagcatga ggacttcggg aagaagagcc ttctggcaac
     8401 gagcgcgctg gcggtggcgc gcctgctcct gtcgatccgc gaccgcgacc ggccgcgcct
     8461 gaccgctctc ttcctccgtt ccctgctcct ggtcgcggcg tgcggagccc tgttcctcat
     8521 ggcgcaggcc gccgatcgcg gtggcgcttt ggtgtatcgg cacggcctgg cggtcgagcg
     8581 cgcgccggtc cctcccggcg acctctgacc tcgatcgaga tctagggcgg ccgtgggatg
     8641 tcgtagatgt agtaggcgtg cgccacgacg gcccgcggct tctggtgctc gacccagttc
     8701 tgcggaccgt cgccgtactt gaccgcgaga tggccccggg tccgcgcggc cagatgcgtg
     8761 gagacggcgt agagccccat cggcagacct cgcatcaact cgtcggtgcc cagccggtcg
     8821 tatccgatgc cgtaataatc cggccgggcg gtgccgaaat acgccagtcc gatcctccgg
     8881 cccggggcgt tctgcctcag ccaggcggcg agcgtcttca acccctgccc ccagtcgacg
     8941 ttgctgtcgt cgagccacag cggcccgcag gcggtgccgc cgtcgcggcc gagccgggac
     9001 ggggcggtcg tgagacacgc gctctcgttg aagaaggcga ggtggtccgg cgcgatcccc
     9061 cacgcgttcc aaccgagcca gacgccgagg accgcgagcc ccgcgcgccc ggcgatcgct
     9121 ttcgactccc acagagcctt cgccccaact ccgccgacga gcgccaggaa cggcagcgcc
     9181 gggatgaggt agcggaaccc gaggttgtgc gacaggaagg tgtagaagaa gaacagcccg
     9241 gccggcggca cgaggaggaa ggcgcggtcc atcgccgtca cgcccccccg gaacatgagc
     9301 gcccacaccc ccgtcgcgac cagcaggatc gccgggagcg gctccttcag gagccaggcg
     9361 acgaggtagt acgaccagaa atgcggccgg aaggtccccc ccatgtacgg ccagtaggag
     9421 ggatcgtgat cggcgttcac ccgcagaagc cccgtccagt agagcgcggg gctgtggaag
     9481 aaataggagg cccagatgac cagtcccgcg acgacgacga gccccgcgaa ggagtagaga
     9541 caccagacga cgcgcggcgt catcgcctcg ccggcgtagg gatcgaacag cgtgctcggc
     9601 cggtgctgca ccgaagcggg gatccagcgg gcggcggcga gaaagaacac gagcaggacc
     9661 ggcagcagca agacgccggt gaacttcgac gcgagcgccg ctcccagcgc gagaccgcag
     9721 aggagcaggc gcggcatcgt ccggtgctgg agccagcgcc acagggtcca caccgacagg
     9781 aacgcgaaga gagcgaaccc ggcgtcggtg gtcacgagga cgccgtgcgc caccatcgac
     9841 ggatcgagcg ccagaagtcc cacggcggcg agcccggcga acggcccgag catctgccgt
     9901 ccccacgcga acaccgacca gccgagcagg agcgtcagga gcaggaacgg cgcgcgcgcc
     9961 aggagcatca cgcggtccgg gtcgttgagg tacaggacgc tgctccccac ctcccactgc
    10021 atcagcggcc cgggatcggg acccgattcg atccagttcc gctccgacac cggccagcgg
    10081 atgccgcccc acaggagcgg caccgcggcg atctccttga ggaggggcgg atgctggaga
    10141 ttgagcttga agtcgccggt ctcgaggtac gacatcccgg ctccgacgtg cgccggctcg
    10201 tcgaaggtcg gcgccatgcg cgcgatcccc ggggccgcct gccaggcgta caacgccagg
    10261 aggagacagg cgaagaatcc gctcgttttc gcgagaccac gcgttttcat ggggcgcgat
    10321 gctagcacca acgtcgacgc cggaagaggg tgatagagtc tcggacttcc ttggggggcg
    10381 cttgaacggg gcgacatcgc gcgacgaggc ggcgccggcc ggcgacgcgg cgaccgctcc
    10441 cccgctcgca gggcccgacg cgttcctcga gcggcgctcc ggtcccctcg cgttcgccct
    10501 gctcgcgctc ctcgtgctgc aggggatcgc cttcatcgcc gaatcgtcgc agacgagcga
    10561 cgaggcggcg cacctctcgg ccggctactc ctacctgacg cggggggatt tccgcctcaa
    10621 cccggagcac ccgcccctca tcaaggagct ggcggcgctg ccgctgctgc cgctcgggct
    10681 cgacttcccc gagggaccgc tctggcagat ggcggaggag tggaacatcg gccggatctt
    10741 cgtgcacgag aa
//
LOCUS       DASRIQ010000626         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1562709, whole genome shotgun sequence.
ACCESSION   DASRIQ010000626 DASRIQ010000000
VERSION     DASRIQ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562709"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2118
                     /locus_tag="VFD06_15595"
     CDS             <1..2118
                     /locus_tag="VFD06_15595"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZN05008.1"
                     /translation="PAHRAGGAMGELTAGVLAELFNRWGALVVAVALLGGGMLLLGRT
                     SFVDGLRGVMRRVAGMFRGAWLAWVRHREARRREEQRRAIADRQRERLERAARAEESS
                     EPAVEPRAAAPIPLLQELPEASPSPPRTEMPVRPAVEPLKTLRKGGRAEKSPAADAAG
                     KPGEKQPEFDFIEAMPNYTPPPLSLLEPLTRGSQVDEKELVETARLITEKCREFEVNG
                     QVIAIQPGPVVTVFEFKPDAGIKYSRIVSLADDLCLAIKAESIRIDRLAGKSTVGIEV
                     PNRTREVISLRELLSSERFQRAPASLTLALGKEINGEPYYADLARMPHLLVAGSTGSG
                     KSVCLNTLLTSILYKASPEEVKFIMIDPKRLELGVYENIPHLLTPLVTDTKKAGNALR
                     WAVVEMERRYKQLAEAGVRSIDQYNVMMRRAMGRVDKPEAPAPEPLPAPGAGAVAAEA
                     GATDVAGAEPPASGRKHFLPGEKPAILPYVVILIDELADLMMTTGREVEEAITRLAQM
                     ARAVGIHLILSTQRPSVDIITGVIKANFPCRIAMRVSSKVDSRTILDSNGAEHLLGHG
                     DMLFIPPGSSRLVRLHGPLVTEIEATRIVTFLKKQAKPQYDETVTRDEAEAGPGGASG
                     AGGEEQDPAYDQAVRLAVQQGQISVSHIQRRLKLGYARAARLVDMMEANGIVGPADGS
                     KPREVLVEPDFLARLDQMREEQG"
BASE COUNT          382 a          819 c          832 g          311 t
ORIGIN      
        1 ccggcgcacc gggcgggggg ggcgatgggc gagctgaccg cgggtgtcct cgcggagctg
       61 ttcaaccgct ggggggcgct ggtggtggcg gtggcgctcc tcggcggcgg catgctcctc
      121 ctcggacgga cgtcgttcgt cgacgggctg cgcggcgtca tgcgccgcgt ggccgggatg
      181 ttccgggggg cctggctcgc ctgggtgcgc caccgcgagg cgcgccgccg cgaggaacag
      241 cgccgcgcca tcgccgacag gcagcgggag cgcctggagc gcgcggcccg cgccgaggag
      301 tcgtcggagc cggcggtcga gccgcgcgcc gccgcgccga tcccgctgtt gcaggagctg
      361 ccggaagcgt ccccctcccc gccgcgcacc gagatgccgg tgcgcccggc ggtcgagccg
      421 ctcaagaccc tccgcaaggg ggggcgcgcc gaaaagagcc cggccgccga tgcggcgggc
      481 aaaccggggg agaagcagcc ggagttcgac ttcatcgagg cgatgccgaa ctacacccct
      541 cccccgctgt cgctgctcga gccgctgacg cgcggctcgc aggtggacga gaaggagctg
      601 gtggagacgg cgcggctcat caccgagaaa tgccgcgagt tcgaggtcaa cggccaggtg
      661 atcgccatcc agccgggacc ggtggtgacg gtcttcgagt tcaagccgga cgccggcatc
      721 aagtacagcc gcatcgtcag cctcgccgac gacctgtgcc tggcgatcaa ggcggagtcg
      781 atccgcatcg accggctggc cgggaaatcg actgtcggga tcgaggtgcc gaaccggacg
      841 cgcgaggtga tctcgctgcg cgagctgctg tcgagcgagc gcttccagcg ggcgccggcg
      901 tccctgacgc tggccctcgg caaggagatc aacggcgagc cgtactacgc cgacctcgcc
      961 cgcatgccgc acctgctggt cgcgggctcg accggttcgg ggaagagcgt ctgcctcaac
     1021 acgcttctga cctccatcct ctacaaggcg agccccgagg aagtgaagtt catcatgatc
     1081 gaccccaagc gtctcgagct cggggtgtac gagaacatcc cgcacctcct gactccgctc
     1141 gtcaccgaca cgaagaaggc gggcaacgcc ctgcgctggg cggtggtcga gatggagcgc
     1201 cgctacaagc agctggcgga ggcgggggtg cgcagcatcg accagtacaa cgtgatgatg
     1261 cgacgcgcga tggggcgggt ggacaagccg gaggctccgg ctccggagcc gctgcccgca
     1321 ccaggcgccg gggccgtcgc ggcggaggcg ggcgccaccg acgtggccgg cgccgagccc
     1381 ccggcgagcg ggcgcaagca tttcctgccc ggcgagaaac cggccatcct tccctacgtc
     1441 gtcatcctga tcgacgagct ggccgatctc atgatgacga ccggccgcga ggtggaggag
     1501 gcgatcacga ggctggcgca gatggcgcgc gccgtcggca tccacctgat cctttcgaca
     1561 cagcggccat cggtcgacat catcaccggc gtcatcaagg cgaacttccc ctgccgcatc
     1621 gccatgcgcg tctcgtcgaa ggtggactcg cgcaccatcc tcgactccaa cggcgccgag
     1681 cacctgctgg gccacggcga catgctgttc atcccaccgg gatcgtcgcg cctcgtgcgg
     1741 ctgcacggac cgctggtgac cgagatcgag gcgacccgca tcgtcacctt cctcaagaag
     1801 caggcgaagc cgcagtacga cgagacggtc acccgcgacg aagccgaggc gggcccgggc
     1861 ggcgcctccg gcgccggagg tgaggagcag gatccggcct acgaccaggc ggtccgcctc
     1921 gccgtgcagc agggacagat ctccgtgtcg cacatccagc gccgcctcaa gctcggctac
     1981 gcccgcgccg cccgcctcgt ggacatgatg gaggccaacg gcatcgtcgg ccccgccgac
     2041 ggcagcaagc cgcgcgaggt cctcgtcgag cccgacttcc tcgcccgcct cgaccagatg
     2101 cgcgaagagc agggctgatt ccggacccac gaagagcgcg aacgttcctg attgcaggat
     2161 cggtgcgatc gggatatagg gggcggtata ccccgtcggg tacacttgga cggaaccgac
     2221 catgagcctg ccgactcatt cgtcagctgc caaagacacc caaactcaac accgtgccac
     2281 gaacgcggtg acggcgctgg cggccatcgc catgggggtg gtctcggctg gcgcctgccc
     2341 ggtc
//
LOCUS       DASRIQ010000627         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2588312, whole genome shotgun sequence.
ACCESSION   DASRIQ010000627 DASRIQ010000000
VERSION     DASRIQ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588312"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="glgP"
                     /locus_tag="VFD06_15600"
     CDS             <1..868
                     /gene="glgP"
                     /locus_tag="VFD06_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZN05009.1"
                     /translation="KKVEAIPDAELWETHLDLKRRLHRFLLARSRRRLLDGRSSPPQV
                     LASGALFDPETLTLGFARRFATYKRASLIFRDPGRLRALVCDPRRPMQIVFAGKAHPA
                     DEPGKHVLQEIYRAAEDRGFAGRIAFVEDYGLHSAQHLVQGVDVWLNTPRPPLEASGT
                     SGQKAALNAVPSLSVLDGWWAEGFDGENGWSIGSPDPGPDGDERDAADAADLYHRLES
                     EVIPAYYERNGNGLPHRWLGIMRRSLATLTPRFSARRMVKEYIDVLYRPGWEAQGQAA
                     AERAMEGPRGAD"
     gene            complement(814..>4141)
                     /locus_tag="VFD06_15605"
     CDS             complement(814..>4141)
                     /locus_tag="VFD06_15605"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HZN05010.1"
                     /translation="TNSGILSRLTWNGSTWVKLDLVRGLPRSEENHAANGLVLINSGA
                     TLLVAEGGNTNMGAPSNNFALLPEYALSAAILSIDLAAIGSTTYDLPTLDDPAPSRNT
                     ATPGVDTNDPFGGNDGANQARLVAGGPVQVYAPGFRNPYDVVVTRAGRLYTIDNGPNS
                     GWGDVPQGEGGTNCTNATREPGVTHDDNLHYITGPGFYGGHPNPTRANTSNTFNGQSP
                     VPASNAVECDYRNPVTQDGALATFGFSTNGLAEYTAANFAGAMDGNLIAAAFDNTIYR
                     IVLSANGTAATLVAPLFSSVGATPLDVWAQGTAGPFPGSIWVADYSANSIVVYEPNDF
                     VSCTGADHPGLDEDGDGFSNADEIDNGTNPCSAGDVPPDWDGDHVSNLNDPDDDNDGL
                     PDTSDRFALDGQNGATTNLPLLLTWDNDAPSAGGLLDLGFTGLMTNGTSNYASLFNPG
                     AMTAGGAAGVVTVDAVPEGDANGALNTQQYGFQAGVHVTAATPLFTAHTRILAPFAGL
                     TPANFQTMGLFLGTGDQDHYVKLVVGADGGTGVTFGQEDGGPLVTRPAAPVTLPGPDW
                     IDLYLTIDPVAHTVTPSFIVTTGNVPGPVESLGGPAAIPASWLAGPQGLAVGILSTSR
                     GPGAPFPATWDFLKVRAGPAVECSADPDCADANPCTTDVCQSYTCLHLNNTDPCNDGL
                     SCTSGDICAAGVCGGTDACPAGQMCDIGTNGCILLNVDLDNDGLVGAADPCPTAPRNL
                     CFGPVAIETAQSRAIRINAGVAGGECSGTKVDCSGRTWNADYGYSSASAVSTCNLGGG
                     GEGCVISGIPTLFGCEDETTEDLFQCDHFDDEFAPELSYAFAVPNGPYLVNLYFANTY
                     TGTDAVGSRVFNILIEGATAYSNFDQVAAAGGSGIAVVRSKVVNVADGTLNISFGHVT
                     ENPALKAIEVFRQDRDTDGSFPPADCNDLNAAVHPGASDVTCDGVDQNCNGAADEGYV
                     PSAEVCDGADNDCNGVIDDAPPLATIGSLTVVTGAAPTDAVLSWGVIAGASGYDLVRG
                     DVRTLVEGGGAFATAIDQCLADNTASQGLVESGTPAVGEGFFYIQRGINCGGGSWDEG
                     GTGQSAPRGPSIALSAAACP"
BASE COUNT          571 a         1398 c         1534 g          638 t
ORIGIN      
        1 gaagaaggtg gaggcgatcc cggacgccga gctgtgggag acgcacctcg acctgaagcg
       61 ccgcctgcac cggttcctgc tggcccgctc gcggcgccgg ctcctcgacg gacgctcctc
      121 cccgccccag gtcctggcgt cgggggcgct gttcgatccg gagacgctca ccctcgggtt
      181 cgcccggcgt ttcgcgacgt acaagcgggc gtcgctcatc ttccgcgacc cggggcgcct
      241 gcgggccctc gtgtgcgacc cgcgccggcc gatgcagatc gtcttcgccg gcaaggcgca
      301 cccggccgac gagccgggca agcacgtcct ccaggagatc taccgggcgg ccgaggaccg
      361 cggcttcgcg gggcgcatcg ccttcgtcga ggactacggg ctgcactccg cgcagcacct
      421 ggtgcagggg gtggacgtct ggctcaacac gccccggccg ccgctcgagg cgagcgggac
      481 gagcggccag aaggcggccc tcaacgccgt cccgagcctg agcgtgctcg acggctggtg
      541 ggcggagggg ttcgacggcg aaaacggctg gagcatcggc tcacccgacc ccggccccga
      601 cggtgacgag cgggacgcgg ccgacgccgc cgacctctat caccgcctcg aatccgaggt
      661 gatcccggcg tactacgagc gcaatggcaa tgggctgccg caccgctggc tcgggatcat
      721 gcggcggtcg ctggcgacgc tcaccccccg cttctcggcg cgccgcatgg tgaaggagta
      781 catcgacgtg ctgtaccggc ccggctggga ggctcagggg caggcggcgg cggagagggc
      841 gatggagggg ccgcgcggcg ccgactgacc ggtccccccc tcgtcccagg agccgcctcc
      901 gcagttgatc cctcgctgga tgtagaagaa cccttccccg accgccggag tccccgactc
      961 gaccagaccc tgcgaagcgg tgttgtcggc gaggcactga tcgatggcgg tcgcgaacgc
     1021 tccgcccccc tcgaccagcg tccgcacgtc gccgcgcaca aggtcgtagc ccgaggcgcc
     1081 cgcgatcacg ccccacgaga ggaccgcgtc ggtcggtgcg gcgccggtca ccacggtgag
     1141 cgagccgatg gtcgcgagcg gcggggcgtc gtcgatgacg ccgttgcagt cgttgtccgc
     1201 gccgtcgcag acctcggccg acggcacgta cccctcgtcg gcggcgccgt tgcagttctg
     1261 gtccacgccg tcgcaggtga cgtcgctcgc gccggggtgc accgccgcgt tgaggtcgtt
     1321 gcagtcggcc ggcggaaagg agccgtcggt gtcccggtcc tggcggaaca cctcgatcgc
     1381 cttgagggcc gggttctcgg tgacgtgacc gaaggagatg ttgagcgtgc cgtcggcgac
     1441 gttcaccacc ttcgagcgca cgacggcgat gccgctgcct ccggccgccg ccacctggtc
     1501 gaagttggag tacgcggtcg ccccctcgat caggatgttg aacacacgag aaccgacggc
     1561 gtccgtcccg gtgtaggtgt tggcgaagta caggttcacc aggtacgggc cgttcgggac
     1621 cgcgaaggcg tacgacagct cgggcgcgaa ctcgtcgtcg aagtgatcgc actggaagag
     1681 gtcctcggtc gtctcgtcct cgcagccgaa gagggtcggg atgccgctga tgacgcaccc
     1741 ctcgccgcct cccccgaggt tgcaagtgga gaccgccgag gccgaggaat agccgtagtc
     1801 ggcgttccag gtcctcccgg agcagtccac cttcgtcccg gagcactcgc cgccggcgac
     1861 gccggcgttg atccggatgg cgcggctctg cgccgtttcg atggcgaccg ggccgaagca
     1921 gaggttccga ggcgcggtcg ggcacgggtc ggcggccccc acgaggccgt cgttgtcgag
     1981 atcgacgttc aacaggatgc agccgttcgt gccgatgtcg cacatctgac cggcgggaca
     2041 ggcgtcggtg ccgccgcaga ccccggcggc gcagatatcg ccgctcgtac aggacaggcc
     2101 gtcgttgcac gggtcggtgt tgttcaggtg caggcaggtg tacgactggc agacgtcggt
     2161 cgtgcacggg ttcgcgtcgg cgcagtcggg atcggcggag cactccacgg ccgggccggc
     2221 ccgtactttg aggaagtccc aggtggccgg gaacggagcg cccggcccgc gcgacgtcga
     2281 gaggatcccg accgccagcc cctgcggccc cgccagccag gacgccggga tcgccgccgg
     2341 gcccccgagc gactcgaccg gccccggcac gttgccggtc gtgacgatga acgacggcgt
     2401 cacggtgtgg gccaccgggt cgatggtcag gtagaggtcg atccagtcgg ggcccggaag
     2461 cgtcacgggt gcggccggcc gggtcacgag cggtccgccg tcctcctgcc cgaacgtcac
     2521 tccggttccc ccgtccgcgc cgacgacgag cttcacgtag tgatcctggt ctccggtgcc
     2581 gagaaacagg cccatcgtct ggaagttcgc cggcgtcagc cccgcgaagg gggcgaggat
     2641 gcgggtgtgg gcggtgaaca gcggcgtcgc cgcggtcacg tgcacgccgg cctggaagcc
     2701 gtactgctgc gtgttgagcg cgccgtttgc gtccccctcc ggcacggcgt ccacggtcac
     2761 gacccccgcc gcgccgccgg cggtcatcgc cccggggttg aacaggctgg cgtagttgga
     2821 cgtgccgttg gtcatcagac ccgtgaaccc gagatcgagc aggccgccgg cgctcggcgc
     2881 gtcgttgtcc caggtcagga gcagcggcag gttggtcgtc gcgccgttct gcccgtcgag
     2941 ggcgaagcgg tcgctcgtgt cgggaaggcc gtcgttgtcg tcgtccgggt cgttcaggtt
     3001 ggagacgtgg tcgccgtccc agtcgggcgg gacgtccccc gccgagcatg ggttcgtgcc
     3061 gttgtcgatc tcgtcggcgt tcgagaaacc gtcgccgtct tcgtcgaggc cgggatggtc
     3121 cgctccggtg caggagacga agtcgttcgg ctcgtacacc acgatggagt tcgcggagta
     3181 gtcggcgacc cagatcgaac cggggaacgg cccggccgtc ccctgcgccc agacatcgag
     3241 cggcgtcgcc ccgaccgacg agaagagggg cgccaccagc gtcgcggccg tgccgttcgc
     3301 cgagagaaca atgcggtaga tggtgttgtc gaaggcggcg gcgatcaggt tcccatccat
     3361 ggcgccggcg aagttcgccg cggtgtactc ggcgaggccg ttggtcgaga agccgaacgt
     3421 cgccagcgcg ccgtcctgcg tcaccgggtt gcggtagtcg cactccaccg cattcgatgc
     3481 ggggaccggc gactggccgt tgaaggtgtt cgaggtgttg gcgcgcgtcg ggttcgggtg
     3541 cccgccgtag aagccggggc cggtgatgta gtgtaggttg tcgtcgtggg tcacgcccgg
     3601 ctcacgggtc gcgttggtgc agttcgtccc cccttccccc tgcggcacgt ccccccagcc
     3661 ggagttcggg ccgttgtcga tggtgtagag cctgccggcg cgggtgacga cgacgtcgta
     3721 ggggttgcgg aatccggggg cgtacacctg caccgggcct cccgcgacca ggcgggcctg
     3781 gttggcgccg tcgttgccgc cgaacggatc gttggtgtcc acgccgggcg tggcggtgtt
     3841 gcgggacggc gccgggtcgt cgagggtcgg caggtcgtag gtcgtcgagc cgatcgcggc
     3901 caggtcgatc gacaggatcg ccgccgacag cgcgtattcc ggcaggaggg cgaagttgtt
     3961 cgagggggcc cccatgttgg tgttcccccc ctccgccacc aggagcgtgg cgccggagtt
     4021 gatgagcacg agtccgttcg cggcgtgatt ctcttcggag cgaggcagcc cgcggacgag
     4081 gtcgagcttg acccacgtgc tgccgttcca ggtgagacgc gagaggatgc cggagttggt
     4141 g
//
LOCUS       DASRIQ010000628         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1815029, whole genome shotgun sequence.
ACCESSION   DASRIQ010000628 DASRIQ010000000
VERSION     DASRIQ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815029"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1916
                     /locus_tag="VFD06_15610"
     CDS             57..1916
                     /locus_tag="VFD06_15610"
                     /inference="COORDINATES: protein motif:HMM:NF037701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05011.1"
                     /translation="MIPLAAFLLTMAAQEGWIAATDEAAVRRSGAWTAGRHRYAAADA
                     LATREDGAALDVAFRGRGLSAVFDTLTVASYGKPELGLVEISIDGGEPRIVHPRATAD
                     EIVLARGLPDGDHRIRLVHRRSDLGAGARVLGFRVLPQDSGDLAFVVTGESNGQLVDV
                     RAVLTREGGTVRNTLLRNWLTGECRLAGLPPGEGYVLELRASGWKTLRRGGITIRPDR
                     ETALTPIHLEREWDVPEGAVRFPALGRPSVARPGGIFRLRLWAYQAEVGEVRVVRRQG
                     PAVVSRVCRFQEDPGAAYYYYKEGTVTLPGDVPAGLYDLEALVGTQKRASLGSVMVVT
                     DFPRDPVFLGVGHLDTWGQNQAEYLERVADLANLLAPDLVLVSNEVNAAYVAGALHRL
                     EIPFVINFGNHQVHGHERWFGDPVGIVDFGPGLSILNFGREWAGGCADADALFAARPK
                     AAVKVINAFEHNAPVAEFLDRHRIQLLHDGHGPGARVMMMGATPTLRVVKPDSESFRL
                     IRFKDGRAVSFTYRGEAESAIPFARKAETPVRVRFEPSNDGTANRVTARWSNDLAEDY
                     PNGRVVFVLPPGTYRADGGRIESAVESDDKKFVLLTVRVDLTAKSTGSVQVGP"
     gene            2081..>2309
                     /locus_tag="VFD06_15615"
     CDS             2081..>2309
                     /locus_tag="VFD06_15615"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZN05012.1"
                     /translation="MSLPGCAAPPQDGQKGRKLKVIFVGAHPDDMEGAAGGTMARYAD
                     QGHEVVSLYLTRGEAGMKGKSHEEAGTIRSGQ"
BASE COUNT          368 a          769 c          816 g          356 t
ORIGIN      
        1 tcaacgagcc aggcgtcgct ccgaaacgag tcgcgtccgg acattgttga aggaacgtga
       61 tcccgctggc cgcgttcctc ctgacgatgg ccgcccagga gggctggatc gcggcgaccg
      121 atgaggcggc ggtgcggcgc tcgggcgcct ggaccgctgg ccgccatcgc tacgccgcgg
      181 cggacgccct cgccacccgc gaggacggcg cggctctcga cgtcgccttc cgggggcgcg
      241 ggctatcggc cgtcttcgac accctgacgg tcgcgagcta cgggaagccc gagctgggac
      301 tcgtggaaat ctcgatcgac ggcggggagc cccggatcgt ccatccccgg gccacggccg
      361 acgagatcgt cctcgcgcgc gggctgcccg acggcgacca tcggatccgc ctggtccatc
      421 ggcgctcgga cctgggcgcc ggagcgagag tcctgggatt cagagtgctt cctcaggatt
      481 cgggcgacct cgccttcgtc gtgaccggcg aatccaacgg ccagctcgtc gacgtccgtg
      541 ccgtcctcac ccgcgagggc ggaaccgtcc gaaacacgct cctccggaac tggctgaccg
      601 gcgaatgccg gctggccggc ctgccgcccg gagagggcta tgtcctggag cttcgggcct
      661 cgggctggaa gaccctccgc cgcgggggga tcacgatccg gccggaccgg gaaacggcac
      721 tgacgccgat ccacctggag cgcgaatggg acgtgccgga gggggcggtg agattcccgg
      781 cgctgggacg gccgtcggtc gcgcggcccg gcggaatatt ccgactgaga ctctgggcct
      841 atcaggccga ggttggcgag gtccgcgtgg ttcgccgcca agggcccgcc gtcgtctccc
      901 gcgtctgcag gttccaggaa gatccgggcg cagcctatta ctactacaag gaggggacgg
      961 tcacgctccc cggggacgtc cccgcgggcc tttacgatct cgaggcgctc gtcggcacgc
     1021 agaagcgggc atcgctcgga agcgtcatgg tggtgacgga cttcccgcgc gatcccgttt
     1081 ttctcggagt cggccacctg gacacgtggg gacagaacca ggccgaatac ctcgaacggg
     1141 tagcggatct cgccaacctc ctggcgccgg acctggtcct cgtgtcgaat gaggtcaacg
     1201 ccgcgtatgt cgcgggggcg ctgcaccggc tcgagatccc cttcgtgatc aacttcggga
     1261 atcaccaggt ccatggtcac gagcgctggt tcggggatcc ggtcggcatc gtcgatttcg
     1321 gtcccggcct ttcgatcctc aatttcggac gcgagtgggc gggcgggtgc gcggacgccg
     1381 acgcgctctt cgcggcccgg ccgaaagccg ccgtgaaggt gatcaacgcc ttcgagcaca
     1441 acgcgccggt ggcggagttc ctggaccgcc accggatcca gctcctccac gacggccacg
     1501 ggcccggcgc gcgcgtgatg atgatgggcg cgacgcccac cctgcgggtc gtgaagcccg
     1561 acagcgagag cttccgcctg atccggttca aggacggccg cgcggtttcc ttcacctacc
     1621 ggggcgaggc ggagtccgcg atccccttcg cgcgcaaggc ggagactccc gtgcgggtcc
     1681 ggtttgagcc ttcgaacgac gggacggcca accgggtgac cgcccggtgg tccaacgatc
     1741 tcgcagagga ctacccgaac ggccgggtcg tcttcgtcct gccgccgggg acctatcggg
     1801 ccgacggagg gcggatcgag agcgccgtcg aaagcgacga caagaagttc gtgctcctga
     1861 ccgtccgggt cgacctgacg gcgaagtcca ccggcagcgt ccaggttggc ccgtgatccg
     1921 gcccgtcaca atttgaggcc aatcctcccg ggacgagtta atagtttaca gacaccttct
     1981 cagagtccgg ggcggaccat tctggagaca cgccatggaa gaactgaccc gtcgcgatgc
     2041 tttgagattg tcgggatggt cggcagcggc tgccggagcg atgtcgctcc ccgggtgcgc
     2101 ggcccctccc caggacggac agaagggccg gaagctgaaa gtgatcttcg tgggcgccca
     2161 tccggacgac atggagggtg ccgccggcgg aaccatggcc cgctatgccg atcaggggca
     2221 cgaggtcgtc tcgctctatc tcactcgagg ggaagcgggg atgaagggaa agtcccacga
     2281 ggaggcggga accatccggt cgggccagg
//
LOCUS       DASRIQ010000629         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2589251, whole genome shotgun sequence.
ACCESSION   DASRIQ010000629 DASRIQ010000000
VERSION     DASRIQ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2589251"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1217)
                     /locus_tag="VFD06_15620"
     CDS             complement(75..1217)
                     /locus_tag="VFD06_15620"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN05013.1"
                     /translation="MKAVRKTAPRPGALEWADVAVPECGPQDVLLKVRAASLCGTDAH
                     IYQWDPSIASKIAAATGGLARPLTLGHEFCGEVVEVGRDVRGVGARSREPIRKGDFVS
                     AESHIVCGECYQCRHGEFHVCARERIIGVHRDGGFAEYIAIPARCAWINDPTVVPVEV
                     ACVEEPFGNAVHAATEYDPRDRLVVLFGAGPIGLFAIIVAAAVGAARIIAIDTSPYRL
                     DLAKKVGADVVLKTEPQPREDQAARDRERERLVGLLRDAASPWEIDLVLEMSGAPDAL
                     DAGLRAVRRGGKVVLFGLPKENAVTIARYSEDVIFGGVTLKGIIGRKLYDTWERTRAL
                     TNRREVREKIQSVITHTYPLSRYEEAFARMIGRDSGKVVMTVGGEG"
     gene            1348..1971
                     /locus_tag="VFD06_15625"
     CDS             1348..1971
                     /locus_tag="VFD06_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05014.1"
                     /translation="MGTAGWEGMRLFAKLVMGCLLAGATAIGPALAVGNVNFVGGERG
                     LDRDLWGDLDEQDVFGVMVDYTAPTWPVRLEGAIFFSSQTTDVVEPVFFTETEVEGRV
                     SELAVGLNKTWDKRGRMRPFVGGGLAWVVAEREIRSEFFGDDHEDSDALGVYVHGGIF
                     WRVGATFNLGFDARLMGTTDVGFFGSSGDASYGQVGILLGFGWPPHP"
     gene            complement(2008..2985)
                     /locus_tag="VFD06_15630"
     CDS             complement(2008..2985)
                     /locus_tag="VFD06_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN05015.1"
                     /translation="MTSRIVPAITLSLLLVTAVSPVPAGAEEPPTPDEDADAPSSAAP
                     LGASETGEPPATAGTNAGDGDRPYWRTNLFKRLFSDQKFLFTDWIGQELHRPGFTLPL
                     VAGVALAATSHGDDLSQPDLRLERRFNASVSEEGTGTARAFTNLGDAGTGIVLLGATY
                     LIGRFGGHDRLAEASSLSAEALINTGLWTTILKRATARSRPVGGAEGRFFDYQPAPGQ
                     SNESFPSGHAAGAFALATVFSGLYGEDHRWVPWVAYGTAGLIGVSRVGLGRHFTSDVL
                     VGGVIGNSFGRMVLARRDGPAAARRVQTTFTPAFDPVTHEAEVDLTLRW"
     gene            3139..3885
                     /locus_tag="VFD06_15635"
     CDS             3139..3885
                     /locus_tag="VFD06_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05016.1"
                     /translation="METSPLLTVSCIRPGLWRWTAPHPEWTPEKDRPGGWGRMVGSVY
                     CEPPDGVGPVVLIDPLAPPPDTPEAERFWAALDRDLARAGRPLLLVIGNGYHARGAAG
                     FSRRYAERYGADLHVPADAVTRLRLEAARPYGGGGLPGTDLVAFPIEGMDGGETALYL
                     RGHGALVFADAVIGAGEGRLAVAPPSWAGDGEAAAALYRSRFRASLEPLLELEADIVL
                     PSHGEPVISGAGTALAAALAGPAWGESPAV"
     regulatory      4078..4160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4216..>4445
                     /locus_tag="VFD06_15640"
     CDS             4216..>4445
                     /locus_tag="VFD06_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05017.1"
                     /translation="MSPRRILRAVPLLLSLAFWNSTSQAATLSAPYSLTATAVSSGQI
                     NLSWLDNNNSENRFTIERSLDPLTGWVHIGTNS"
BASE COUNT          670 a         1509 c         1567 g          699 t
ORIGIN      
        1 cggcagccgg aagatcccct tctccttgag cgcggcgatc tccccttcga ggaaccgctc
       61 cagacgctga ttcatcatcc ctccccgcca acggtcatca ccaccttgcc gctgtcccgg
      121 ccgatcatcc gcgcgaacgc ctcctcgtag cgggacaacg ggtaggtgtg ggtaatgacc
      181 gactggatct tctcccgcac ctcgcgccgg ttcgtcaggg cgcgcgtcct ctcccaggtg
      241 tcgtagagct tgcgcccgat gatccccttg agcgtcacgc cgccgaagat cacgtcctcg
      301 ctgtagcgcg cgatggtcac ggcattctcc ttcgggaggc cgaacaggac caccttgccg
      361 ccccgccgca ccgccctcag tccggcgtcg agcgcgtccg gggccccgct catctcgagc
      421 acgagatcga tctcccacgg cgacgcggcg tcgcgcagca gaccgacgag gcgctcccgc
      481 tcccggtcgc gcgccgcctg gtcctcacgg ggctgcggct cggtcttcag aacgacgtcg
      541 gcgccgacct tcttcgcgag gtcgaggcgg tagggggacg tgtcgatcgc gatgatgcgc
      601 gccgccccca ccgcggcggc gacgatgatg gcgaagagcc cgatcggccc ggcgccgaac
      661 agcaccacca ggcgatcgcg cgggtcgtat tcggtggcgg catgcaccgc gttgccgaac
      721 ggctcctcga cgcaggcgac ctccaccggg acgaccgtcg gatcgttgat ccaggcacag
      781 cgggccggga tggcgatgta ctcggcgaag ccgccgtcgc ggtgcacgcc gatgatgcgc
      841 tcgcgggcgc agacgtggaa ctcgccgtgc cggcactggt agcactcgcc gcagacgatg
      901 tgcgactcgg cggagacgaa gtcccctttt cggatcggct cccggctgcg tgcgccgacg
      961 ccgcgcacgt cgcgccccac ctccaccacc tcgccgcaga actcgtggcc gagcgtgagc
     1021 ggccgggcga gcccgccggt ggcggcggcg atcttggaag cgatggaggg gtcccactgg
     1081 tagatgtggg cgtcggtgcc gcacagcgag gcggcccgca ccttgagcag gacgtcctgg
     1141 ggcccgcact ccggcaccgc cacgtcggcc cattcgagcg cccccgggcg gggtgcggtc
     1201 ttgcgcacgg ctttcacgca atctctcctc aacggcagat cggagcggga ttatagagga
     1261 ggttgcagca ggtgggacga aaagcgtccc tggcttcgcg tttggaggcg cggaaggcgg
     1321 ggggcgcggc gggggggcgt agaatcggtg ggcacagcgg ggtgggaggg gatgcggctc
     1381 ttcgcgaagc tcgtcatggg atgcctgctc gcgggcgcaa cggccatcgg gccggcgctc
     1441 gcggtgggga acgtgaactt cgtcggaggg gaacgcgggc tcgatcggga cctgtggggg
     1501 gatctcgacg agcaggatgt cttcggcgtg atggtcgact acaccgcgcc gacctggccg
     1561 gtgcggctcg agggggcgat cttcttctcg tcgcagacga ccgacgtcgt cgagccggtg
     1621 ttcttcacgg agaccgaggt cgaggggcgc gtgtcggagc tggcggtcgg actcaacaag
     1681 acgtgggaca agaggggccg catgcgtcct ttcgtcggcg gcggcctggc atgggtcgtg
     1741 gcggaacggg agatccgttc ggagttcttc ggggacgacc acgaagattc cgatgcgctg
     1801 ggtgtctacg ttcacggggg gatcttctgg cgcgtcggag cgaccttcaa tctgggattc
     1861 gacgcccgcc tgatggggac gaccgacgtc gggttcttcg gctcgtccgg cgatgcgagc
     1921 tatgggcagg tcggaatcct cctcggcttc gggtggcccc cgcatccctg accccggcgc
     1981 cgcgcgacac gcctcgcctg cccccgctca ccagcgcagc gtcagatcca cctccgcctc
     2041 gtgcgtcacc gggtcgaagg cgggcgtgaa ggtcgtctgg acgcggcgcg ccgctgcagg
     2101 gccatcgcgg cgggccagga ccatgcggcc gaagctgttg ccgatgaccc ctcccacgag
     2161 gacgtcgctc gtgaagtgtc tccccagccc gacgcgcgag actccgatca ggccggccgt
     2221 gccgtacgcc acccagggga cccagcggtg gtcctcgccg tagagccccg agaagacggt
     2281 ggcgagggcg aacgcccccg ccgcgtgccc ggacggaaac gactcattcg actggccggg
     2341 ggccggctgg tagtcgaaga agcgcccttc cgccccgccg accggccggc tgcgcgccgt
     2401 cgcccgcttg aggatcgtcg tccagagccc cgtgttgatc agcgcctcgg ccgagaggct
     2461 cgaggcctcg gccaggcgat cgtggccgcc gaagcggccg atcagatacg tcgcaccgag
     2521 cagcacgatg ccggtccccg cgtctccgag gttggtgaag gcgcgcgccg tccccgtgcc
     2581 ctcttcgctg acggaggcat tgaaccggcg ctccagcctc aggtcgggct ggctcaagtc
     2641 gtcgccgtgc gacgtggcgg cgagagccac cccggccacg agcggcagcg tgaacccggg
     2701 ccgatgcagc tcctgtccga tccagtcggt gaacaggaac ttctgatcgg agaacagccg
     2761 cttgaacagg ttcgtgcgcc aataggggcg atccccgtct cccgcattcg ttccggccgt
     2821 cgccgggggt tcgcccgtct ccgaggcgcc gagcggcgcg gcggacgagg gggcgtcggc
     2881 gtcctcgtca ggagtgggtg gctcctccgc ccccgcgggg accggtgaaa cggcggtgac
     2941 gagcaggagc gacagggtga tggccggcac gatccgtgag gtcacgcacc cagactaggg
     3001 tccggtgggg gaccgaagcc agccgagact gaaagacccc tgaggaattg aagaaatatt
     3061 gaccgtttgt tcggtgccgt gctggaaagc gttcgggatc cggcgaccgc agcgcccgat
     3121 ccataatttc ctgacgccat ggagaccagt ccgctcctca ccgtgagctg catccgtccc
     3181 gggctgtggc gctggaccgc gccgcatcct gaatggaccc cggagaagga tcgacccggg
     3241 ggctggggac ggatggtcgg ctcggtctac tgcgagccgc ccgacggcgt ggggcccgtc
     3301 gtcctcatcg atccgctcgc accgccgccg gacacgcccg aggcggagcg gttctgggcc
     3361 gcgctcgacc gggacctcgc gcgagccggc cgaccgctcc tcctcgtcat cggtaacggc
     3421 taccacgcgc gcggcgccgc cgggttctcg cgccgctacg cggaacggta tggcgccgat
     3481 cttcacgtcc cggcggacgc agtgacgagg ctgcggctcg aggcggctcg tccgtacggg
     3541 gggggagggc ttcccggaac agacctcgtg gcattcccca tcgaaggaat ggatggcggc
     3601 gagacggcgc tgtatctccg cggtcatggt gccctggtgt tcgcggacgc ggtgattggg
     3661 gcgggggagg gccgtctcgc ggtcgcccct ccctcctggg cgggggatgg cgaggcggcc
     3721 gcggcgctct atcgcagccg cttccgcgcc tccctcgagc ccctgctcga gctcgaagcg
     3781 gacatcgttc tgccgtcgca cggggagccg gtgatctcag gggccggcac cgcgctcgcc
     3841 gcggccctcg ccggcccggc ctggggggaa tcgcccgcgg tctgatctgc ccatccacgg
     3901 ggcagcgggt gcggccttcg ctcgaatccc cagcgaggga tttgtgattg tcggttacca
     3961 cagcggactt gctggggtcc gatccgtgcg tgggtctcat gcaaacacac gtgatacgac
     4021 tcgatctctg ctggcctagg tcttgctgta attcgctgcg ttatcgcgaa ggccacttct
     4081 cgataagggc aaacccgacc gtaaggcggg gacgcaaagc cacgggtcag accgaaagat
     4141 ctgacagccg ggttaccgaa ggaagggagt accccttggt cttgaggggc cttcagaacc
     4201 ggggggaggc cttccatgag cccgaggcga atactacggg cagtaccgct cctgttatcc
     4261 ctcgcattct ggaattcgac ctcacaggcg gccaccctgt cggcgccgta ctctctgacc
     4321 gcgacagccg tctccagtgg ccagatcaac ctcagctggc tcgacaacaa caactcggag
     4381 aaccgcttca caattgaacg cagtctcgac ccgttgaccg ggtgggtgca catcggcacc
     4441 aactc
//
LOCUS       DASRIQ010000630         3853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2328521, whole genome shotgun sequence.
ACCESSION   DASRIQ010000630 DASRIQ010000000
VERSION     DASRIQ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328521"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFD06_15645"
     CDS             <1..445
                     /locus_tag="VFD06_15645"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN05018.1"
                     /translation="EMNVPVKRFAGEALRALSESSWPGNVRQLENVVERAVALSFGRE
                     ELGREDLPRDLLEEGALEMPLLHVGGDGFSLDEVLATYEQRMLYQALEHSGWVKTRAA
                     EMLKIKRTTLIEKMKRLGIPLKGRVAGEGAEAAETVAAAVAIRRS"
     gene            588..1280
                     /locus_tag="VFD06_15650"
     CDS             588..1280
                     /locus_tag="VFD06_15650"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZN05019.1"
                     /translation="MASLLMVDHALLFRLLESTCARRSGWSLRSALTADDLLEKARQQ
                     RPDVIVLSGVPTCDAIAAASRLRADASLRAVPILAIGRGADLAACARAGADAALEWPA
                     AEAELEASLANLMHETRRSARRRAARLAATLQTGAGLLRVWLRDIGPGGAFFATRQGP
                     AEGAAVEVTLRLPDAPGSAQVVARGVVVRRVEEDAGSHRVAGVAVRFTDPDPERTRRL
                     EAFVRGPIPPEA"
     gene            1296..1688
                     /locus_tag="VFD06_15655"
     CDS             1296..1688
                     /locus_tag="VFD06_15655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN05020.1"
                     /translation="MTETVMVVDDDRDILDLARLVLEDGGYRVIAAPSGQAALDRLAQ
                     SRPDLILLDVNMPDMDGWQVLRLLKVDERTQEIPVALFTIKSQVRDRVLGLQSGACDY
                     IAKPFAQEELLARVRHILDSVRGGQVGA"
     gene            1685..3016
                     /locus_tag="VFD06_15660"
     CDS             1685..3016
                     /locus_tag="VFD06_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZN05021.1"
                     /translation="MSRRDDDVSVPDWAGEAERLRAGLLRLKGALHDRVTGLFSYHLF
                     MDQLASHADHGLLGVLALEFPTLASVEAARGFEVGDRFLRDASRALVAIRGDALPEDA
                     LLALDGAHGDSFLVFVREAGDTEGIAARLDDLAARVAAGMDQRLAAKNGDRPRLECAV
                     GQALVSPAPAARFERRLHQAIREARGASRRGAERRHDARGAEARAILQSGALTTHYQP
                     IVDMEHGTIMGYEALTRGPAHSPLEGPDALFATCQQARLQSELDRICRCNAVRNARGI
                     DRSKKLFLNSLPESLGAPGFNEGGFREALSEAELEPRHLVLEITERTSIDDFEAFGRG
                     LEPLRRQGFLVAIDDVGTGYSSLQTLTEVQPEFIKVDLSLVKNIHRSLIKQELVHSLL
                     QVGGRIGAHVIAEGIESEEEARTLRRCGVRYGQGYWFARPGPGFPSLMRQG"
     gene            3029..>3853
                     /locus_tag="VFD06_15665"
     CDS             3029..>3853
                     /locus_tag="VFD06_15665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN05022.1"
                     /translation="MEAFPLAPLLLGLAAAGLLAAILVCAIALARLRGALRRFVARTE
                     ALRRHPLVGEVVPEPDSPIADVERSLQSLVDGLRDEARKAQGKVSSLQALAEGPADLG
                     LIGLDGEWIVASFSRGAVRLTGWPADEMVGRHVEALFAAGEWERLLVKLARRSLREEG
                     FSETVRLQRRDGTEAPVGLSAGAAGSTGGTLLVIRDLRAERELERRLRVSEERHRRLV
                     EEVQDGVLLVQEGRIAYANPALARLLGATVESLRNRPFKDLLETRDLLRVVDLLARA"
BASE COUNT          503 a         1384 c         1430 g          536 t
ORIGIN      
        1 cgagatgaac gtgccggtga agcgcttcgc gggggaggcg ctgcgcgcgc tctccgaatc
       61 gtcctggccc gggaacgtga ggcagctcga gaacgtcgtc gaacgtgccg tggcgctgtc
      121 gttcggccgg gaggagctgg gccgcgagga cctgccgcgc gaccttctgg aggagggcgc
      181 gctcgagatg ccgctcctgc acgtcggcgg ggatggcttc tcgctcgacg aggtgctggc
      241 cacctacgag cagcggatgc tctaccaggc gctcgagcac tccggctggg tgaagacccg
      301 cgccgccgag atgctgaaga tcaagcgcac caccctcatc gagaagatga agcgcctcgg
      361 gattccgctc aagggacgcg tcgccggcga gggggcggag gcggcggaga cggtcgcggc
      421 ggcggtcgcg atccggcgct cctgaacggg gcgggtggcg tgcgtctcct tgtggcacgc
      481 gtcggacctg tggtaacgtc cggccgcgac ttcgggagcg tcgccgcatc gcatccagcc
      541 ccctgccgtc gcgcactccc gcgcggcctg ccataactga gacgatcatg gcctctttgc
      601 tgatggtcga tcacgccctg ctcttccggt tgctggagtc cacctgcgcg cgccggtcag
      661 ggtggagcct gcgatccgcc ctgaccgccg acgatctcct ggagaaggcg cggcagcagc
      721 gccccgacgt catcgtcctg agcggcgttc cgacctgcga cgccatcgcc gccgcctcgc
      781 ggctgcgcgc cgacgcgagc ctccgggcgg tgccgatcct ggcgatcggc cgcggcgccg
      841 acctcgcggc ctgcgcgcgc gccggcgccg acgcggcgct cgagtggccc gccgcggagg
      901 cggagctcga ggcctcgctc gcgaacctca tgcacgaaac gagacgcagc gcccgccgac
      961 gtgcggcgcg gctggcggcg acgcttcaga ccggggccgg gctcctgcgc gtgtggctcc
     1021 gcgacatcgg cccgggcggg gcgtttttcg cgacgcggca ggggccggcg gaaggggccg
     1081 ccgtcgaggt gaccctgagg ctgccggacg ctcccgggag cgcgcaggtc gtggcgcgcg
     1141 gcgtcgtcgt acggcgcgtc gaggaggacg ccggctcgca ccgcgtcgcg ggggtggcgg
     1201 ttcgcttcac cgatccggat cccgagcgta cccggcgcct cgaggcgttc gtgcgcggac
     1261 cgatccctcc ggaagcgtga catgagaggc cgtcgatgac cgagacggtc atggtggtgg
     1321 acgacgaccg ggacatcctc gatctggccc gcctcgtcct ggaggacggt ggctaccgcg
     1381 tgatcgccgc accgtcgggc caggccgcgc tcgaccgcct ggcgcagagc cgccccgatc
     1441 tcatcctgct ggacgtcaac atgccggaca tggacggctg gcaggtcctg cgtctcctga
     1501 aggtggacga gcgcacccag gagatcccgg tcgccctctt caccatcaag agccaggtgc
     1561 gcgatcgcgt cctcgggctg cagagcggcg cctgcgacta catcgccaag ccgttcgccc
     1621 aggaggagct cctggcgcgg gtccgccaca tcctcgacag cgtgcgcggc gggcaggtgg
     1681 gggcgtgagc cggcgcgacg acgacgtctc cgttcctgac tgggcggggg aggcggagcg
     1741 cctgcgagcc gggctcctgc gcctcaaagg ggcgctgcac gaccgggtca cagggctctt
     1801 ctcctaccat ctcttcatgg accagctggc gagccacgcc gaccacgggc tactgggggt
     1861 gctggcgctc gagttcccga ccctggcttc cgtcgaggcg gcgcgcggct tcgaagtcgg
     1921 cgaccgcttt ctgcgcgatg cctcccgggc gctcgtggcg atccgcggcg acgccctgcc
     1981 ggaagacgcc ctgctcgccc tcgacggcgc gcacggggac agcttcctcg tcttcgtccg
     2041 cgaggccggc gacaccgaag ggatcgccgc gcgtctcgat gatctcgccg cgcgcgtcgc
     2101 cgccgggatg gatcagcggc tcgcggccaa gaacggcgac cggccgcgcc tggagtgcgc
     2161 ggtcggccag gcgctcgtca gcccggcccc cgcggcccgc ttcgagcggc gcctgcacca
     2221 ggcgatccgc gaggcccgcg gggcctcgcg gcgcggcgcc gagcgccgtc acgacgcccg
     2281 cggcgcggag gcccgggcga ttctgcagtc gggagccctg acgacccact accagccgat
     2341 cgtcgacatg gagcacggga cgatcatggg atacgaggcg ctcacccgcg ggccggcgca
     2401 cagcccgctc gaagggccgg acgccctgtt cgccacctgc cagcaggccc gcctgcagtc
     2461 ggagctggat cggatctgcc gctgcaacgc cgtgcgcaac gcccgcggca tcgaccgaag
     2521 caagaagctg tttctcaact cgctgcccga gtcgctcggc gctcccggct tcaacgaagg
     2581 cgggttccgc gaggcgctgt cggaggcgga gctcgagcca cggcatctcg tcctcgagat
     2641 caccgagcgc acctcgatcg acgacttcga ggcgttcggt cgcgggctgg agccgctgcg
     2701 ccggcagggg ttcctcgtcg ccatcgacga tgtcggcacc ggctactcgt ccctgcagac
     2761 cctcaccgag gtccagcccg agttcatcaa ggtggacctc tccctcgtga agaacatcca
     2821 ccgcagcctc atcaagcagg agctggtgca ctccctgctt caggttgggg gacggatcgg
     2881 cgcgcacgtg atcgccgagg ggatcgagtc ggaggaagag gcgcgcacgc tgcgccgctg
     2941 cggagtgcgc tacggccagg ggtactggtt cgcccgcccg gggcccggct tcccctcgct
     3001 gatgcgccag ggctgagggg gaatcctcat ggaggcgttc ccgctcgcgc cgctcctgct
     3061 cggcctggcc gccgccgggc tgctcgccgc catcctggtc tgcgccatcg ccctcgcgcg
     3121 cctgcgcggc gcgctccgcc gcttcgtcgc ccggaccgag gcgctgcgcc gccacccgct
     3181 cgtcggggag gtggtccccg agccggacag cccaatcgcc gacgtggagc gctcgctgca
     3241 atcgctcgtg gacggactgc gggacgaggc gcggaaggcg caggggaagg tgtcgagcct
     3301 gcaggcgctg gccgaagggc cggccgacct cgggctcatc gggctcgacg gcgaatggat
     3361 cgtggcctcc ttcagccgcg gcgcggtccg gctcaccggc tggccggcgg acgagatggt
     3421 cggacgccac gtcgaggcgc tgttcgcggc gggggagtgg gaacggctgc tcgtgaagct
     3481 ggcgcgccgg agcctccgcg aggaagggtt ctcggagacg gtccgcctgc agcgccgcga
     3541 cggcaccgag gcgccggtcg gcctgtcggc cggcgcggcc gggagcaccg gcggcacgct
     3601 tctcgtgatc cgcgacctgc gcgccgagcg cgagctcgag cggcgcctgc gcgtctccga
     3661 ggagcgccat cgccggctcg tggaggaggt ccaggacggc gtgctgctcg tgcaggaggg
     3721 gcggatcgcc tacgccaacc cggcgctggc gcgcctgctc ggagcgacgg tcgagtcgct
     3781 gcggaaccgc cccttcaagg acctcctcga gacgcgcgat ctcctgcgcg tcgtcgacct
     3841 gctggcgcgc gcg
//
LOCUS       DASRIQ010000631         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1029111, whole genome shotgun sequence.
ACCESSION   DASRIQ010000631 DASRIQ010000000
VERSION     DASRIQ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029111"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFD06_15670"
     CDS             <1..725
                     /locus_tag="VFD06_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN05023.1"
                     /translation="PIVDTWWQTETGMILITPLPGITTTVPGSATHPFPGVAAEVLDE
                     AGRPVPAGGGGYLAITRPWPAMMQGVYGDYDRYVKTYWSKWDIKTYFPGDGARRDKHG
                     NFWLLGRVDDVLNVAGHRIGTMEVESALVDHPAVAEAAVVGKSHELKGQALAAFVTVK
                     DGRPADDALNAELKQHVVQKIGALARPDDIIFTPELPKTRSGKIMRRLLRDIAEGRAL
                     GDVTTLADAAVLEALKQRYQED"
     gene            complement(771..>2336)
                     /locus_tag="VFD06_15675"
     CDS             complement(771..>2336)
                     /locus_tag="VFD06_15675"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN05024.1"
                     /translation="LGLCCEEARDLIGTQRSAASMTLHGDRSRSIVVDGSDGEPGGWR
                     TDGDEQDLSDLYALVCPPNRPMRANADDLRRNPAFRRTTSAADLPAVRNWLAAPLTGL
                     PDNNVGLLQLADRSEGDFTADDEAILVQLAQMACVAIDNAWLVEHLREANRRKDEFLA
                     TLAHELRNPLAPLSNGLQVMKLSGNDPKAVETSRVMMERQIEQMVRLIDDLMDVNRIS
                     RGRIELRRQRIDVARVVTQAVETSAPLIEAGRHTLTVDMPHGPLVVDGDETRLAQVLS
                     NLLNNAAKYTEPGGRIHIAAERQKGKVVISVKDTGVGIPKTMLPRIFDLFTQVDKSLE
                     RAQGGVGIGLSLVKGLVELHGGNVEAKSEGHGKGSEFIVRLPVVDEAADPAPKPEKSM
                     TGQLGGYRVLVADDNVDSATSLAMLLRLMGNEVHTAHDGLQAVRVARELQPDLALLDI
                     GMPKLNGYETCQRIKEESGGRKVLLVALTGWGQDEDIRRSHLAGFDRHLVKPVEPAAL
                     EKLLGSMSARRAT"
BASE COUNT          344 a          809 c          801 g          382 t
ORIGIN      
        1 gcccgatcgt ggacacctgg tggcagaccg agaccgggat gatcctcatc acgcccctcc
       61 ccggcatcac gaccaccgtc cccggctcgg cgacgcaccc gttcccaggt gtcgccgccg
      121 aggtcctgga cgaggccggc cggccggtgc cggcaggagg cggcggctac ctcgccatca
      181 cccgtccctg gcccgcgatg atgcaggggg tctacggcga ctacgaccgc tacgtgaaga
      241 cctactggtc gaagtgggac atcaagacct acttcccggg cgatggcgcc cgccgcgaca
      301 agcacgggaa cttctggctg ctcggccgcg tggacgacgt tctcaacgtc gcgggacacc
      361 ggatcgggac gatggaggtc gaaagcgccc tcgtcgatca ccccgccgtc gccgaggccg
      421 cagtggtcgg caagagccac gagctcaagg gccaggccct cgcggcgttc gtgacggtga
      481 aggacggccg gcctgcggac gacgcactga acgccgagtt gaagcaacat gtcgtccaga
      541 agatcggcgc cctcgcccgc cccgacgaca tcatcttcac gccggagctt cccaagaccc
      601 gttccggcaa gatcatgcgc cgcctcctgc gggacattgc ggaaggacgc gcgctcggag
      661 acgtgaccac tctcgccgac gcggccgtcc tggaggcgct gaagcagcgc taccaggaag
      721 actgacatcg cactcagctg tcgaaggttc gaggcgtgtc gaggctggag ttacgtcgcg
      781 cgcctggcgg acatcgatcc cagcagcttc tccagcgccg cgggctcgac cggcttgacg
      841 aggtggcggt cgaagcctgc gaggtgggat cgccggatgt cctcgtcctg accccagccg
      901 gtcagcgcga cgaggagtac cttccggccg ccggactcct ctttgatgcg ttggcaggtc
      961 tcgtagccgt tcaacttcgg catcccgata tcgaggagcg ccaggtcggg ctgcagctca
     1021 cgcgcgaccc ggaccgcctg caggccgtcg tgggcggtgt gcacttcgtt tcccatcagg
     1081 cgcagcagca tcgcgaggct cgtcgcggag tcgacgttgt cgtcggcgac gagcacgcgg
     1141 tagccgccga gctggccggt catcgacttc tcgggcttgg gggccgggtc ggcggcttcg
     1201 tccacgaccg gcaggcggac gatgaactcg cttcccttcc cgtgaccttc gctcttcgcc
     1261 tcgacgttcc cgccgtgcag ctcgacgagc cccttcacga gcgacaggcc gatgccgaca
     1321 cccccctggg ctcgctcgag cgacttgtcc acttgggtga acaggtcgaa gatgcgcggc
     1381 agcatggtct tcgggatgcc gacaccggtg tccttcaccg agatgaccac tttccccttc
     1441 tggcgctccg cggcgatgtg gatgcgaccg ccgggctcgg tgtacttggc ggcgttgttc
     1501 aggaggtttg acagcacctg cgccaggcgg gtctcgtcgc cgtccacgac gagggggccg
     1561 tgcggcatgt ccacggtcag agtgtggcgg ccggcctcga tgagcggagc gctcgtctcg
     1621 accgcctgcg tcacgacgcg cgcgacgtcg atccgctgcc ggcgcagctc gatgcggccg
     1681 cggctgatcc ggttgacgtc catcaggtcg tcgatgagcc gcaccatctg ctcgatctgc
     1741 cgctccatca tgacgcgcga ggtctcgacc gccttcgggt cgttccccga gagcttcatc
     1801 acttgaagcc cgttgctcag cggcgccagt gggttgcgca gctcgtgggc gagcgtcgcg
     1861 aggaactcgt ccttgcgccg gttcgcctcg cgcaggtgct cgacgagcca ggcgttgtcg
     1921 atggcgacgc acgccatctg cgccagctgc accaggatcg cctcgtcgtc ggcggtgaag
     1981 tctccctcgc tcctgtccgc gagctgcagc agcccgacgt tgttgtcggg gagccccgtg
     2041 aggggcgcgg cgagccagtt gcgcaccgcc ggcaggtcgg cggcactggt ggtgcggcgg
     2101 aaggcgggat tgcggcgcag gtcgtcggcg ttggcgcgca tcggccggtt cggcgggcac
     2161 accagcgcgt agaggtcgga gaggtcctgc tcgtcaccgt cagtgcgcca tccccccggc
     2221 tccccgtcgg agccgtccac gacgatcgag cgggaccgat ccccgtgaag ggtcatgctg
     2281 gcggccgagc gctgggtccc gatgagatcg cgcgcctcct cgcaacagag gcccag
//
LOCUS       DASRIQ010000632         9585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_491028, whole genome shotgun sequence.
ACCESSION   DASRIQ010000632 DASRIQ010000000
VERSION     DASRIQ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491028"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..428)
                     /gene="queF"
                     /locus_tag="VFD06_15680"
     CDS             complement(78..428)
                     /gene="queF"
                     /locus_tag="VFD06_15680"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZN05025.1"
                     /translation="MPSKPSRDLETFPNPRPGRDYEISFECPEFTCLCPKTGQPDFAT
                     IRIRYVPDRLCVELKSLKLYLWSFRDEGHFHEDVTNRILDDLVRLLEPKRLTVTGDFN
                     VRGGIHTVVTVAHP"
     gene            complement(432..1301)
                     /locus_tag="VFD06_15685"
     CDS             complement(432..1301)
                     /locus_tag="VFD06_15685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZN05026.1"
                     /translation="MPVCRPFRPSLPASFRLQPLVLAAALAIPAAASAEPIQEGKTVS
                     YKVRNEQVQAYLVRPAKIGPTPGVVVIHEWWGLNDQIKRVADRIAEEGFMAIAPDLYR
                     GKIGTDAGLAHELMRGLNEGYAIEVVTGAVAHLRGIEARERRTPTPTSPRLAVGTLGF
                     CMGGRISLATALSGADVQAAVMYYGSVETNKEALAPLQIPLLGIFGNEDRGIPLDQIR
                     AFQAALQDSGKDATVLVYPGVGHAFFNEDRPSYDSEAAKDSWERTKQFLRANLLIDGA
                     PAPAAKKPRTDLP"
     gene            complement(1409..2875)
                     /locus_tag="VFD06_15690"
     CDS             complement(1409..2875)
                     /locus_tag="VFD06_15690"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN05027.1"
                     /translation="MTRRLWIGGQFTEGAGGAPIEVVDPATEEVVDAVPRGGAADVDR
                     AVAAAKAAFPGWKRTSAWERADLLIEAARRLRAAREDLAVTLTRETGRTLRKNRGYVD
                     WSATCLEYYAGLVRERRGRVIPSSESGQLNLVLKEPLGVVGCLVPWNYPLLLLVWKVA
                     PALAAGNTVVIKPASQTPLATLDLHRVFDHLPAGVVNILTGSGGEVGEALVRHPDVPC
                     IAFTGSTEVGRALMRLGAERIKKLHLELGGSDAFIVCADADLDTAARACAWTAFLNAG
                     QVCTSAERIYVERSAHDRFVEALVKHTRRLVIGPGMDEKTEVTPLIARRERDRIEGRI
                     AEAAKAGATVAAGGRRPPYLAKGWFYEPTVLVGVRQGNPLLAEEIFGPVAPVIPFDGF
                     DEGLRLANDSPYGLGATLFTNDPRRVRRYYDEIEAGNVWVNDPLVDNPAGPFGGMKQS
                     GLGRELGEEGLEEFQQTKHVHWDFEARPKPWWFPWEPA"
     gene            2962..5013
                     /locus_tag="VFD06_15695"
     CDS             2962..5013
                     /locus_tag="VFD06_15695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN05028.1"
                     /translation="MRPRFAAAILAAALLIPCAAAAQEQPSKARFTVGKMLDWERVSD
                     PQIAPDGARVVYVRQWVDQKADKWVSNLFVIEDDGRRERLLTRGFSPRFSPDGSRLAY
                     FAEVEGSGTQIHVLDLRSSGGPTQVTRLLETPSDLRWSPDGRTLAFLMPVAKKPSWKI
                     DLPDAPKGAAWTEAPRIIETTHYRADREGFLKDAWVHLFVVPAGGGTPRALTEGNWNV
                     GGRADGIPESPGIDWTPDGKEILFDGLMDEDADRRFRESHLYAVTVATGAIRRLTSTR
                     GPWTLPRVSPDGKQVAFTGFPWTESTYHTGDLYVMGIDGKGMRLLSGNLDRDIDDLHW
                     AADSSGVYASVEDRGARNVLFLPLQGTRREITRGAQILSLSSVSRSGVAVGVRSSSTE
                     PDDVVRYRLADGARLEPLTRVNEDVLQGLTLGTVEEITARSGDGTLVQGWLVKPPDFD
                     RSKTYPLILHIHGGPHAMYGVGFNTSFQNLASNGYLVLYTNPRGSTGYGTAFGNAIDD
                     AYPSVDFDDLMTMVDAVEARGIVDTRREYVTGVSGGGVLSAWCTSHTTRFAAAAVRAP
                     VIDWISFAGTTDISSWGYFRYRGRFWDDPKKWLTHSPLMHVGKVKTPTLLMTGELDLR
                     TPIGQTEEYYQALRAVGVPTAMIRFNGEYHGTGSKPSNWMRTQLYLLSWFQRWPAAAG
                     S"
     gene            4989..6227
                     /locus_tag="VFD06_15700"
     CDS             4989..6227
                     /locus_tag="VFD06_15700"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN05029.1"
                     /translation="MAGRCRVLTEAAATTPKRPGPRVVVIGAGAFGGWTALWLVRRGA
                     RVTLVDARAAGNIQASSGGETRVTRAVYGANAAAMRLAIRAMDLWKEEQSRLGVELYR
                     ERGVLWMTTTPDDSYLEAALPLLRAAARPFEEVTPRDAATRWPAMSFDDARRVVLEPM
                     AGYLLARRASETVARQVAAEGGTVRQADVRPPALSDARPPGGTAAEPRLDAVTMHDGE
                     RLEADAFVFALGPWLGSFFPGLIGERVRPTRQELFTFATPPGDARFFDDALPTWADLG
                     ETLRYGIPGPAPTSGRGEDEARGMKFGDDARGPLFDPTAGDRTPSAEGEQFARAYLAR
                     RLPALKSPRLQSAQVCQYENTADNQYLIDRHPHAANVWIAGGGSGHGFKNGPAVGEML
                     AEMVLGGRVGDPAFALWRLA"
     gene            complement(6625..7449)
                     /locus_tag="VFD06_15705"
     CDS             complement(6625..7449)
                     /locus_tag="VFD06_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN05030.1"
                     /translation="MSRFTTQDGTEIYYKDWGTGQPIVFSHGWPLCSDSFESQMLSLA
                     SRGYRCIAHDRRGHGRSTQPWNGNDMDTYADDLARLLETCKVDNAFLVGFSTGGGEVA
                     RYIGRYGSKRVSKIALVSAVCPLMLKTEQNPSGLPREVFDGLRTSCLTDRSQFYRDFA
                     NGPFFGFNRPGARVSQGFVDAFWLQGMMAGHRNALDCIRAFSETDFTADLKKCDVPAL
                     VVHGDDDQVVPIAATAKVTAKLIKNCKLVVYPGAPHGLCDTHRERLNEDLLGFLKA"
     gene            complement(7645..7896)
                     /locus_tag="VFD06_15710"
     CDS             complement(7645..7896)
                     /locus_tag="VFD06_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05031.1"
                     /translation="MRRPILIASMALLLVPAGTAIAQRRPDLLADRCVLRLPGMDKVV
                     VRTGLPFKTAGDRRLAFDLYLPPESSATALDALIRPLPS"
     gene            8014..9420
                     /locus_tag="VFD06_15715"
     CDS             8014..9420
                     /locus_tag="VFD06_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN05032.1"
                     /translation="MLSESIERRRSRRRAAGRCAATACAILLIEAPGAGAVLAAGSDP
                     GAELSGGIVVLEPSPPEGQRVILCGALIDGRSATPRKNAALLIEGDHIVKLGGRDIAP
                     AGAPVIDLGTATCLPGLMDLHSHPLIDSDSYQVDSLRMSSATKSLKALKAVQANLLAG
                     WTTLRIAGDADVAYAAMDLRRALRDGLFVGPRLAGAAHYISVTGGGGDINFMAPEQRL
                     LADGLVVDGVDAMRKAVREEIKYGSDWIKLLVSGAFMTVGDNPADVHFSRGELEAAVE
                     EARHRNIPVMAHAHAAEAIKMAIRAGVRSIEHGTFVDDEGIRLMVEKGIFLVPTIYVG
                     DYYAERGTDSPEMQKMVDLSRKYRDDFYRRMAKAVKAGVKIAVGSDFGGYDPKVNFGE
                     FRSLVKAGMTPMQAIQAGTRVAAECLRWDDRLGTIEAGKLADIVAVPGDPLSDLSQFE
                     RVNFVMLGGRIVKRPVAR"
BASE COUNT         1472 a         3304 c         3379 g         1430 t
ORIGIN      
        1 acggcggagg aaatatacag cggcctccga gacgggacaa cggcgatcga cgggcgggac
       61 cggggatccg cacccattca ggggtgcgcg accgtgacga ccgtgtgaat ccccccccgc
      121 acgttgaagt cgccggtcac cgtcagccgt ttcggctcca gcaaccggac caggtcgtcg
      181 agaattcggt tggtgacgtc ctcgtggaaa tgcccctcgt cgcggaacga ccagagatag
      241 agcttgagcg atttcagctc gacgcacaac cggtccggga cgtagcggat ccgaatcgtc
      301 gcgaagtcgg gctgtccggt ctttgggcag agacaggtga actccgggca ctcgaaggag
      361 atctcgtagt cccgtcccgg tcgcggattg gggaaggttt cgaggtccct ggacggcttg
      421 ctcggcacgg gttagggcag gtcggtgcgc ggctttttgg ccgcaggcgc gggagcgccg
      481 tcgatgagca ggttcgcgcg caggaactgc ttcgtccgct cccaggaatc cttggccgcc
      541 tccgaatcgt aggaggggcg atcctcgttg aagaatgcgt ggccgacgcc cggatagacc
      601 aggacggtcg cgtctttgcc ggagtcctgc agggctgctt ggaaggcgcg gatctgatcg
      661 agcggaatcc cccggtcctc gttgccgaag atgccgagga gcgggatctg gagcggcgcc
      721 agcgcctcct tgttcgtctc gacgctgccg tagtacatga ctgccgcctg cacgtcggcg
      781 ccggagagcg cggtggcgag ggagattcgc cctcccatgc agaagccaag cgtcccgacc
      841 gccaggcgcg gagaggtcgg cgtcggggta cggcgctcgc gcgcctcgat gccgcgcagg
      901 tgcgcgaccg cccccgtgac gacctcgatc gcgtagccct cgttgaggcc gcgcatcagc
      961 tcgtgcgcca gaccggcgtc ggtcccgatc ttgccgcggt acagatcggg ggcgatcgcc
     1021 atgaacccct cctcggcgat ccggtcggcg acccgcttga tctgatcgtt gagcccccac
     1081 cactcgtgga tcaccacgac cccgggggtc gggccgatct tcgccgggcg gaccaggtag
     1141 gcctgcacct gctcgttgcg caccttgtag gagacggttt tcccctcctg gatcggctcg
     1201 gcggacgccg cagccggaat cgccagcgcg gcggcgagga ccaggggctg caggcggaac
     1261 gatgcgggca gcgatggacg gaacggacgg cagacaggca tggcgcgttc actcccccgg
     1321 aaacggcctc gttgctgttg tgtgaggcgg aggatcgtag cacacgctca ttcgacttca
     1381 caccgagcgg gaactcccat gccgggactc aggcaggctc ccaggggaac caccacggct
     1441 tcgggcgcgc ctcgaaatcc cagtgtacgt gcttggtctg ctggaactct tcgagcccct
     1501 cctcccccag ctcccggccg aggccggact gcttcatgcc gccgaacggc ccggccgggt
     1561 tgtccaccag cgggtcgttc acccagacgt tcccggcctc gatctcgtcg tagtagcgcc
     1621 gcacccgacg cggatcgttc gtgaagaggg tggccccgag accgtacggc gagtcgttcg
     1681 ccagccgcag cccctcgtcg aagccgtcga aggggatcac cggggcgacg gggccgaaga
     1741 tctcctccgc taggagcgga ttcccctggc gcacgccgac cagcaccgtc ggctcgtaga
     1801 accacccctt cgcgaggtac ggcggccgcc gcccgccggc agccaccgtc gcccccgcct
     1861 tcgccgcctc ggcaatgcgc ccctcgatgc gatccctctc gcggcgggcg atgagcggag
     1921 tgacctcggt cttctcgtcc atgccggggc cgatcaccag ccgccgcgtg tgtttcacga
     1981 gcgcctcgac gaagcgatcg tgcgccgacc gctcgacgta gatccgctcc gcgctggtgc
     2041 agacctgtcc cgcgttgagg aaggcggtcc aggcgcaggc gcgcgctgcc gtgtcgaggt
     2101 cggcgtcggc gcagacgatg aaggcgtcgc tcccccccag ctcgagatgg agcttcttga
     2161 tccgctcggc gccgagacgc atcagggcgc ggccgacctc ggtgctgccg gtgaaggcga
     2221 tgcacgggac gtccggatga cggacgagcg cctcgccgac ctccccgccc gacccggtca
     2281 ggatgttcac cactcccgcc ggcaggtgat cgaagacgcg gtggagatcg agggtcgcga
     2341 gcggcgtctg ggaggccggc ttgatgacga cggtgttgcc ggcggcgagc gccggcgcca
     2401 ccttccagac cagaagcagg agcggatagt tccacggcac caggcagccg acgacgccga
     2461 gcggctcctt caggacgaga ttcagctgac cgctctccga agatgggatg acccggccgc
     2521 ggcgctcgcg cacgagcccg gcgtagtact cgaggcaggt ggccgaccag tcgacgtagc
     2581 cccggttctt gcggagcgtc cggccggtct cgcgcgtgag cgtgaccgcc aggtcctcgc
     2641 gcgcggcgcg cagccgccgt gccgcctcga tcaggaggtc ggcccgctcc caggcgctgg
     2701 tgcgcttcca ccccgggaag gcggccttcg ccgcagcgac ggcgcggtcc acgtcggccg
     2761 ccccgccccg cggcaccgcg tccaccacct cctcggtggc cggatcgacc acctcgatcg
     2821 gcgcaccgcc ggccccttcc gtgaactgac cgccgatcca gagtctccgc gtcatgcagg
     2881 gttggcctcc cgggagcgga attgtacggc cttgctaaga tacgtgcctc gtccccatcg
     2941 ctcaggagcc caggaggccg catgcgtcca cgattcgccg ccgcgatcct cgccgccgcg
     3001 ctgctcatcc cctgcgcggc ggccgcccag gaacaaccct cgaaggcccg gttcacggtc
     3061 gggaagatgc tcgactggga gcgggtctcc gatccgcaga tcgctcccga cggcgcccgc
     3121 gtcgtgtacg tgcgccagtg ggtcgaccag aaggccgaca agtgggtgtc caacctgttc
     3181 gtcatcgagg acgacggccg gcgcgagcgc ctcctgaccc gcggcttctc gccccgcttc
     3241 tctcccgacg ggtcgcgcct cgcgtacttc gccgaggtgg aaggaagcgg aacgcagatc
     3301 cacgtcctcg atctccgctc ctccggcggc ccgacccagg tgacgcggct ccttgaaacc
     3361 ccctccgacc tgcgctggtc gcccgacgga cgcacgctcg ccttcctgat gccggtggcg
     3421 aagaagccat cctggaagat cgatctcccc gacgcgccga agggggcggc ctggaccgag
     3481 gccccgcgga tcatcgagac gacgcactac cgcgccgacc gcgaagggtt cctcaaggat
     3541 gcgtgggtgc acctctttgt cgtcccggcc ggcggcggaa cgccgcgggc actgaccgag
     3601 gggaattgga acgtcggcgg acgggccgac ggcatcccgg aatcccccgg gatcgactgg
     3661 acgcccgacg ggaaggagat cctcttcgac gggctgatgg acgaggacgc tgaccggcgc
     3721 ttccgcgagt cgcacctgta cgccgtgacc gtggcgaccg gcgcgatccg ccggctcact
     3781 tcgacccgag ggccctggac gctgccgcgc gtctcgccgg acgggaagca ggtcgcgttc
     3841 accggcttcc cgtggaccga gtccacctac cacaccggcg acctttacgt catgggcatc
     3901 gacgggaaag ggatgcggtt gctgtccgga aacctcgatc gcgacatcga cgatctgcac
     3961 tgggccgccg acagcagcgg ggtgtacgcc tccgtcgagg atcgcggcgc gcgcaacgtg
     4021 ctgttcctgc cgctccaggg aacgcgccgg gagatcaccc gcggtgcgca gatcctgagt
     4081 ctgtcctcgg tgagccggag cggcgtcgcc gtcggcgtgc gttcttcgtc caccgagccg
     4141 gacgacgtcg tccgctaccg cctcgccgac ggggcgcgcc tggagccgct gacgcgcgtc
     4201 aacgaggacg tgctgcaagg gctgacgctc ggcaccgtcg aggagatcac cgcgcgctcg
     4261 ggggacggca ctctggtgca ggggtggctc gtgaagccgc ccgacttcga caggtcgaag
     4321 acctacccgc tcatcctgca cattcacggc ggtccccacg cgatgtacgg cgtggggttc
     4381 aacacatcgt tccagaacct ggcgtcgaac ggctacctgg tcctgtacac caacccgcgt
     4441 ggcagcaccg gatacggaac cgccttcggc aacgccatcg acgacgctta tccctcggtc
     4501 gatttcgacg acctgatgac catggtggac gccgtggagg cgcgcgggat cgtggacacc
     4561 cggcgcgagt acgtcaccgg cgtctctggg ggcggggtgc tgtccgcctg gtgcacgtcg
     4621 cacaccaccc gctttgcggc cgcggcggtg cgcgcgccgg tgatcgactg gatctccttc
     4681 gccggcacga ccgacatctc gtcctggggg tatttccgct atcgcgggcg cttctgggac
     4741 gatccgaaga agtggctcac gcactcgccg ctgatgcacg tcggcaaggt gaagacgccg
     4801 acgctgttga tgaccggcga gctcgacctg cgcaccccca tcggccagac ggaggagtac
     4861 taccaggcgc tgcgggcggt gggggtgccg acggcgatga tccgcttcaa cggcgagtac
     4921 cacggcaccg gctccaagcc ctccaactgg atgcggacgc agctgtacct gctctcctgg
     4981 ttccagcgat ggccggccgc tgccgggtcc tgacggaggc ggcggcgacc acgccgaaac
     5041 ggccgggccc gcgcgtcgtc gtcatcgggg cgggggcctt cggcggctgg accgccctgt
     5101 ggctcgtacg ccgcggcgcc cgcgtgactc tcgtggacgc gcgcgccgcg gggaacatcc
     5161 aggcgtcgag cggcggcgag acccgcgtga cccgcgcggt gtacggggcg aacgccgccg
     5221 ccatgcgcct ggccattcgt gcgatggacc tctggaagga ggagcagtcc cggctcggcg
     5281 tggagctgta ccgcgagcgc ggcgtcctgt ggatgacgac cacgccggac gattcgtatc
     5341 tggaagcggc cctgccgctc ctgcgggcgg ccgcacgtcc gttcgaggag gtgacgcccc
     5401 gggacgccgc gacacgctgg ccggcgatga gcttcgacga cgcgcggcgc gtcgtgctcg
     5461 agccgatggc cgggtatctg ctggcgcggc gcgcctccga gacggtggcc cggcaggtcg
     5521 ccgcggaggg ggggacggtg cggcaggcgg atgtgcgccc gccggccctc tccgacgcgc
     5581 gtccaccggg cggcaccgcc gccgagccgc gcctcgacgc cgtaacgatg cacgacggcg
     5641 agcggctcga ggcggacgcg ttcgtcttcg cgctcggtcc gtggctcggc agtttcttcc
     5701 ccgggctcat cggcgagcgc gtccggccga cgcgtcagga gctgttcacg ttcgcgaccc
     5761 cgcctggaga tgcgcgcttc ttcgatgacg cgctcccgac ctgggccgat ctcggggaga
     5821 cgctgcgtta cggcattccc ggaccggcac cgacttccgg ccggggcgag gacgaggcgc
     5881 gcggcatgaa gttcggcgac gacgcgcgcg ggccgctgtt cgacccgacg gccggcgacc
     5941 gcactccgag cgccgagggg gagcagtttg cccgcgccta cctcgcacgc cgcctcccgg
     6001 ccctgaagag cccgcggctt cagagcgctc aggtctgcca gtacgagaac accgccgaca
     6061 accagtatct gatcgaccgc cacccgcacg ccgcgaatgt ctggatcgcc ggcggcggct
     6121 ccggccacgg cttcaagaac ggtccggcgg tcggcgagat gctcgcggag atggtcctcg
     6181 gcggccgggt cggagacccg gccttcgccc tgtggcgcct cgcctgaccg ccgcccgctc
     6241 gggccggacg cgcgcccgga gcagacgatg ggcggtcgcg agagatcggt cgcgcggcgg
     6301 aggcggcgcg cttcgcccgc ttcgtggcag gagcgccacg aaacgcccgg agcaggcgcg
     6361 ccggcggatg gcgcgcagct gcaggctgtg cggacaccgt cgcacgcggc ggccgcccgg
     6421 gagcgccccc ccggcccgcc gggcgcatcg cgccctccgg ccgttcggga ggaacgggtg
     6481 cgccgggagc gcgcccgcgg ttcgagtcac gttcgaatcc tgtcgtcgag atcggtccat
     6541 ggtcgtcctt cggtgagcgt cccgcccggc acgtcgaagg cgccgcccgc cttcacgacg
     6601 ggcggcgcct ctcccctggt ctcctcaggc cttcaggaac ccgagcagat cctcgttgag
     6661 gcgttctctg tgggtgtcgc acagcccgtg cggcgccccg gggtagacga cgagcttgca
     6721 gttcttgatg agcttggcgg tgactttggc ggtggccgcg atcggcacga cctggtcgtc
     6781 gtcgccgtgc accacgagcg ccggcacgtc gcacttcttc aggtccgcgg tgaagtcggt
     6841 ctccgagaag gcccggatgc agtcgagggc gttgcggtgg ccggccatca tcccctgcag
     6901 ccagaaggcg tcgacgaacc cctgggagac gcgcgcgccc ggacgattga aaccgaagaa
     6961 cgggccattg gcgaagtcgc ggtagaactg cgaccggtcg gtcaggcacg acgtccgcag
     7021 accgtcgaac acctcgcgcg gcagcccgga ggggttctgc tcggtcttga gcatcagcgg
     7081 gcagacggcg gagacgagcg cgatcttcga cacccgcttc gagccgtagc ggccaatgta
     7141 acgcgccacc tcgccgccgc cggtggagaa gccgacgagg aaggcgttgt cgaccttgca
     7201 agtctcgagc agccgcgcca ggtcgtccgc gtaggtgtcc atgtcgttgc cgttccaggg
     7261 ctgggtgctg cggccgtgtc cgcgacggtc atgggcgatg cagcggtagc cgcgggaggc
     7321 gagggacagc atctgcgact cgaagctgtc cgagcagagg ggccatccgt ggctgaagac
     7381 gatcggctgc cccgtgcccc agtccttgta ataaatctcc gtcccgtcct gcgtcgtgaa
     7441 acgactcatg aaaggtgtcc tcctccggcc agcgccgcat ggggaaattc ggatatttgg
     7501 tgtcggcatc gactctattc ggcggtcagg aaacgaccca tcccgataag tgcgtaatcg
     7561 ggccggtgag aatgtggaga agtctgtgga tggaagtgcg ggcacgacgg gaccatgcgc
     7621 ggaagcgggc gctccgcgcg gacttcagga ggggagaggc cggatcagcg cgtcgagggc
     7681 cgtcgcggac gattccggcg gcaggtacag atcgaaggcc agccggcggt cgcccgcggt
     7741 cttgaacgga aggcccgttc ggacgaccac cttgtccatc ccgggcaggc ggagcacgca
     7801 ccggtcggcc agcaggtccg gccgccgctg ggcgatcgcc gtccccgcgg ggacgagcag
     7861 cagcgccatc gatgcgatga ggatcggtcg tctcatggcg gggcctccga agtcccgctc
     7921 atggtacgga gcgccggccg gatcggccgg taaaaagact gtaaaagacg ctgtcgcccg
     7981 cggttagcat ggccgcctcc cgaggaggcg acgatgctca gcgagtcgat cgaacggaga
     8041 aggtcgcggc ggcgcgccgc ggggcggtgc gcggcgaccg cgtgcgcgat cctgctgatc
     8101 gaagcgccgg gagcgggggc ggtcctcgcc gcgggctccg acccaggggc ggaactttcc
     8161 ggaggcatcg tcgtcctgga gccgtccccg ccggaaggac aacgggtcat cctgtgcggg
     8221 gcactcatcg acggccggtc ggcgaccccg cggaagaacg ccgccctcct gatcgaaggg
     8281 gatcacatcg tcaagctcgg cggccgcgac atcgcccccg ccggagcgcc ggtgatcgat
     8341 ctgggcacgg cgacctgtct cccgggcctg atggacctgc actcgcaccc gctcatcgac
     8401 agcgactcct accaggtaga ttccttgagg atgtcctcgg cgaccaagtc cttgaaggcg
     8461 ctgaaggcgg tgcaggcgaa tctcctcgcc ggctggacga cactgcggat cgccggcgac
     8521 gcggacgtcg cttacgccgc gatggatctc aggcgcgcgc ttcgggatgg cctgttcgtc
     8581 gggccccggc tcgccggtgc agcgcactac atctcggtga ccggaggggg cggcgacatc
     8641 aacttcatgg ccccggaaca gcgcctgctc gccgacggcc tcgtcgtcga cggtgtggac
     8701 gccatgcgta aggccgtgcg cgaggagatc aagtacggca gcgactggat caagctcctg
     8761 gtgagcggcg cgttcatgac ggtcggcgac aaccccgccg atgtgcactt cagccggggg
     8821 gagctggagg cggccgtcga ggaagcccgg catcgcaaca tcccggtcat ggcgcacgcg
     8881 cacgccgccg aggcgatcaa gatggcgatt cgcgccgggg tgcgcagcat cgagcacggc
     8941 accttcgtcg acgacgaggg gatccggttg atggtcgaga aggggatctt cctggtgccg
     9001 acgatctacg tcggcgacta ctacgccgag cgcggcaccg actctcccga gatgcagaag
     9061 atggtggacc tcagccgcaa gtaccgcgac gacttctacc ggcgcatggc caaggcggtg
     9121 aaggcgggcg tgaagatcgc ggtcggctcg gatttcggcg gctatgaccc gaaggtcaat
     9181 ttcggggaat tccgatcgct cgtgaaggcg gggatgacgc cgatgcaggc gatccaggcg
     9241 gggacccgcg tcgccgcgga atgtcttcgc tgggacgacc ggctcggaac gatcgaggcg
     9301 gggaagctgg ccgacatcgt cgccgtcccc ggcgaccctc tctccgatct ctcgcagttc
     9361 gagagggtga acttcgtcat gctcggcggg cggatcgtca aacgccccgt tgctcgctga
     9421 gccccgcgac gccgcccgaa aaagatggag cccggtggcg ccgaaggcca ccgggctcct
     9481 gactcaccaa ccgccgcccc ctcccaggtc agtggcggcc agtgagacca actcggtatg
     9541 tcaatgaccg aagtacgttc cgctcaccgc gaatccaggc gtcgc
//
LOCUS       DASRIQ010000633         5437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2591599, whole genome shotgun sequence.
ACCESSION   DASRIQ010000633 DASRIQ010000000
VERSION     DASRIQ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591599"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..538)
                     /locus_tag="VFD06_15720"
     CDS             complement(149..538)
                     /locus_tag="VFD06_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN05033.1"
                     /translation="MSIALDHLIVPSRDRRGAAETLAGVLGVPWAESGAAGPFSPVYV
                     SDGLTLDFDQADGPFPVQHYCFRVAEADFDGIVARLKTRGIPYRSTPHGPVDMKINTD
                     HGGRIVYWNEPDGHVWEALTVSYARQP"
     gene            complement(535..963)
                     /locus_tag="VFD06_15725"
     CDS             complement(535..963)
                     /locus_tag="VFD06_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HZN05034.1"
                     /translation="MIIGAHSIIYSKKPEADRAFLRDVLGLPHVDVGQGWLIFGLPPS
                     EVGVHPSRKNDVHEFYLMVRDVNAFVAAMRKRRVACAKVQDQGYGIMTAITLPGGGKI
                     GVYQPRHARPKSMSVPKTAARRAAGARRGNGGARRGVPAG"
     gene            complement(991..1539)
                     /locus_tag="VFD06_15730"
     CDS             complement(991..1539)
                     /locus_tag="VFD06_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05035.1"
                     /translation="MRPRASWLLLLVLALAAGPAPGQWREDGKVVPDNDWRKSAGSFG
                     AMMMLTEDPEKFFEQWNKPPSPDYKPKITTTDELRRGGHVAALVLFIGCQADSSGNCS
                     VEADYVLRSPDGGVYGEQKNVKVWKDKPAPPDMTLQLGVSVFGLELEQDDPLGTYMFE
                     AKVRDRNAKVEVLLKRPLKVAK"
     gene            complement(1536..2048)
                     /locus_tag="VFD06_15735"
     CDS             complement(1536..2048)
                     /locus_tag="VFD06_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05036.1"
                     /translation="MNRPVPIVLTSILLTALLQAAPVAAKKPQSTPEERAKAVQLTRE
                     LERDPMAQDGPEKRSWLVEFFTKAPDISITMCNLFGPMPGEDHPFFPQVLTQSLFSMG
                     TFIIEHPDKAKDEVAVQTAGMEGALKVYEIHVKAQPEGRLPFLDDLLKKRDAGTLADS
                     MKEAVPKACK"
     gene            complement(2045..2452)
                     /locus_tag="VFD06_15740"
     CDS             complement(2045..2452)
                     /locus_tag="VFD06_15740"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN05037.1"
                     /translation="MAAGAKRWSIAPYFIVDDVVATANWYRDRLGFAYERFWNDPPSF
                     CMVHRGGVVIMLAQLEKPGLKRPNHVVDPEGGALDAYLWVDDADALNTEFKAKGVTIA
                     RDIRDEPYGCRDFEIEDCNGYRLCFGHDTGGGS"
     gene            complement(2490..2933)
                     /locus_tag="VFD06_15745"
     CDS             complement(2490..2933)
                     /locus_tag="VFD06_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN05038.1"
                     /translation="MPNTIRLHRVLKAPPARVYKAFIDPEAMVKWLPPNGFTGKVHQM
                     EARAGGSYRMSFTNFSSGKSHSFGGKYVELVPNERLRYTDKFDDPNLPGEMQTTITLK
                     KVFCGTDLFIVQEGVPEVIPPEACYLGWQESLTLLAKLVEAEIPD"
     gene            complement(2994..3575)
                     /locus_tag="VFD06_15750"
     CDS             complement(2994..3575)
                     /locus_tag="VFD06_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05039.1"
                     /translation="MKAWPALFLCALALSCSPEPAPPKPTPPEPAPPAPAQSPPGAAP
                     SASAPTDCGPAVPEQADNVRTAVLESLISQAASDALQHQVALGPIVLAQEERQPGGGV
                     FVHDVAPDFLERFAGRTPPVGNYSTAFMVEKGRTVRTEAPVAFATGEICWSSPTRALV
                     NARKLSSNANRAFRATVEQRDGAWQVASLGDRR"
     gene            complement(3578..4078)
                     /locus_tag="VFD06_15755"
     CDS             complement(3578..4078)
                     /locus_tag="VFD06_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05040.1"
                     /translation="MSGSWALLSSSGRERVTWHVRAVEGTGADAVLTCDIALSGQFFG
                     ESLGSGRPEAPPDFSLEIRGLLLRLSTLQRLYDHLKRWLDLPLAEMRSTRLRLDCDMG
                     GLFDQDVRLILGERADTLSSGHPVATVKYIVGRMSGELSFVTDPSCLASLCEEVGAAL
                     RRESRP"
     gene            complement(4075..4878)
                     /locus_tag="VFD06_15760"
     CDS             complement(4075..4878)
                     /locus_tag="VFD06_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN05041.1"
                     /translation="MSKAVDLYDNIYSDFGSRAEAEVRREAFGDDIGQSSWMTAEDWL
                     RSADQAQVREGSRVLEVGSGSGGPAVHLAAARGCRVTGVDINAHGVRNGTQLAAERGV
                     ADRVTFQVVDARRPLPFSPATFDAVLSNDAMCHISNRLEVLGEWHRVLRPGGRMLFTD
                     ALVVTGMVSHEEVAMRASIGRYLFVPPGENERLIRQAGFTLLGAEDVTEAAERIAERW
                     RTARERHREALVEREGEANFDGLQRFLECVRRLSAERRLSRFSYLAERG"
     gene            complement(4889..5341)
                     /locus_tag="VFD06_15765"
     CDS             complement(4889..5341)
                     /locus_tag="VFD06_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN05042.1"
                     /translation="MSEARRVSGASKPGANVRQAVPFFRVADMEASLRFYVDGLGFEM
                     TNRWVVDDRVRWCWLRNGDAAVMLQQFAREGHDSWKSEGKVGVGVSICFVCDDAPAFY
                     REVVARGVAASRPLVGNRMWVTSVTDPDGYVLEFESPTDVPEETQYSE"
BASE COUNT          845 a         1882 c         1815 g          895 t
ORIGIN      
        1 aggcagccga gcacgacgcg tgccttctcg cgcacctcga ccgcggcgat gtccttcagg
       61 atcgtgtcgg cgagatcggc gggataatcg cgacggacga tgcgctcgac gtcctgcatg
      121 tcgacatcag gcgcgggctg cttgggcttc atggctgccg ggcataactg acggtgagcg
      181 cctcccagac gtgaccgtcc ggctcgttcc agtagacgat gcggccgccg tgatccgtgt
      241 tgatcttcat gtccacggga ccgtgcggcg tgctgcgata aggaattcca cgggtcttga
      301 gcctcgcgac gataccgtcg aagtcggcct ccgcgacccg gaagcagtag tgctggacgg
      361 ggaacgggcc gtccgcctgg tcgaaatcga gcgtcaaccc gtcgctcacg tagaccgggg
      421 agaagggccc cgccgcgccg ctctccgccc acggaactcc gaggacgccc gcgagcgttt
      481 cggccgcgcc cctccggtcg cgcgagggaa cgatcaggtg gtcgagcgcg atgctcatcc
      541 cgccgggacg ccgcggcgcg ccccgccgtt gccgcggcgc gctccggccg cgcggcgcgc
      601 cgccgtcttc ggcacactca tcgacttggg gcgcgcgtgg cgcggctgat agacgccgat
      661 cttcccgccg ccgggcagcg tgatggcggt catgatgccg tacccctggt cctggacttt
      721 cgcgcaggcg acgcgccgct ttcgcatggc cgcgacgaag gcgttgacgt cgcggaccat
      781 cagatagaat tcgtgcacat cgttcttcct cgacggatgc acccccactt ccgagggggg
      841 cagcccgaag atgagccagc cctggccgac gtccacgtgc ggcaggccga gcacgtctcg
      901 caggaacgcg cggtcggcct cgggcttctt gctgtagatg atcgaatgcg ctccgatgat
      961 catcgcggcg actccttcgg agaataggaa ctacttcgcg accttgagcg gccgcttcag
     1021 caggacttcg accttcgcgt tccggtcccg gaccttggcc tcgaacatgt aggtgccgag
     1081 cgggtcgtcc tgctcgagtt ccagtccgaa aaccgaaacc ccgagctgca gggtcatgtc
     1141 cggcggcgcg ggcttgtcct tccagacctt gacgttcttc tgttccccgt acacacctcc
     1201 gtccgggctg cgcaggacat aatcggcctc gacgctgcag ttcccggagg agtctgcctg
     1261 gcagccgatg aacaggacga gagcggccac gtggccccca cggcgcaatt catccgtggt
     1321 cgtgatcttc ggcttgtaat cgggggatgg cggcttgttc cactgctcga agaacttctc
     1381 cggatcctcg gtgagcatca tcatcgcccc gaacgagccg gcggacttgc gccagtcgtt
     1441 gtccggaacg accttcccgt cctcgcgcca ctggcccggc gccggtccgg ccgccagtgc
     1501 gagaacgagc aggagcagcc aggatgcgcg gggcctcact tgcacgcctt cgggaccgct
     1561 tccttcatgg agtcggccag cgtcccggca tcgcgcttct tgaggagatc gtcgagaaac
     1621 gggagccgcc cttccggctg ggccttcacg tggatctcgt agactttcaa tgcgccctcc
     1681 atccctgcgg tctgcacggc gacctcgtcc ttcgccttgt cggggtgctc gatgatgaag
     1741 gtgcccatcg agaagagcga ctgggtgagc acctgcggga agaagggatg gtcctccccg
     1801 ggcatcggac cgaacaggtt gcacatggtg atgctgatat ccggagcttt cgtgaagaac
     1861 tcaaccagcc aggagcgctt ctcgggtccg tcctgcgcca tcggatcccg ctccagctcg
     1921 cgggtgagct gaaccgcctt cgcccgttcc tcgggcgtcg attgtggctt cttggcggcg
     1981 accggcgcag cctgcaggag cgccgtcagg aggatggacg tcaggacgat gggcacgggt
     2041 cgattcacga tccacctccg gtgtcgtgtc cgaagcagag ccggtagccg ttgcagtctt
     2101 cgatctcgaa gtcgcggcag ccgtacggct cgtcccggat gtcgcgggcg atcgtcaccc
     2161 ctttcgcctt gaactcggtg ttgagcgcgt cggcgtcgtc cacccagaga taggcgtcca
     2221 aggctcctcc ctcggggtcg acgacgtggt tcgggcgctt gaggccgggc ttttcgagct
     2281 gggcgagcat gatgacgact ccgccgcggt ggaccatgca gaacgagggc gggtcgttcc
     2341 agaaccgctc ataggcgaag ccgagccggt cgcgatacca gttggcggtc gcgacgacgt
     2401 cgtcgacgat gaaataaggg gcgatgctcc agcgcttcgc gcctgcggcc atgcgttcgc
     2461 ctccgctccg gtccgcgcct cggactcttc tagtccggga tttccgcctc gaccagcttt
     2521 gcgagcaggg tgagcgattc ctgccacccg agatagcacg cctcgggcgg aatgacctcg
     2581 gggaccccct cctgcacgat gaagaggtcg gtcccgcaga agaccttctt cagcgtgatc
     2641 gtcgtctgca tttcgccggg caggttcggg tcgtcgaact tgtcggtgta gcggagccgc
     2701 tcgttcggca ccagctcgac gtacttgccg ccgaaggagt ggctcttgcc gctgctgaag
     2761 ttcgtgaacg acatcctgta cgacccgccg gcacgggctt ccatctggtg caccttgccg
     2821 gtgaacccgt tcggcggcag ccacttgacc atcgcctcgg gatcaatgaa cgccttgtag
     2881 acgcgcgcgg gcggggcctt caggacgcgg tgaagccgga tggtgttcgg catgatggct
     2941 ccttgtcggg ctgagggcag cagactacct cagaaatccg ccgccgtcct cccctagcgc
     3001 cggtcgccga gggacgccac ctgccacgct ccgtcccgct gctcgaccgt ggcccggaag
     3061 gcccggttgg cgttggacga cagcttccgg gcgttgacga gcgcacgcgt ggggctcgac
     3121 cagcagatct ctccggtcgc gaaggccacc ggcgcttcgg tccgcacggt gcgccccttc
     3181 tcgaccatga acgcggtcga gtagttgccg accggcggcg tgcggcccgc gaagcgctcg
     3241 aggaagtcgg gggcgacgtc gtggacgaac acgccgcctc ccggctggcg ctcctcctgc
     3301 gcaaggacga tgggaccgag cgccacctgg tgctgcagcg cgtccgacgc cgcctgggag
     3361 atgagggact cgaggaccgc cgtgcgcacg ttgtccgcct gctccgggac cgccggtccg
     3421 cagtctgtcg gtgcggatgc ggacggcgcc gccccgggcg gggattgcgc cggggcgggc
     3481 ggcgccggct ccggcggagt cggtttcggc ggcgccggct ccggcgaaca ggacaaggcc
     3541 agggcgcaca ggaacagcgc gggccacgcc ttcatcctca tggccggctc tcccgccgga
     3601 gggccgcgcc gacctcctcg cacaacgacg cgagacacga cgggtcggtg acgaacgaca
     3661 gctcgccgga catccggccc acgatgtact tgaccgtcgc gacgggatgc ccggaggaaa
     3721 gagtatcggc gcgctcgccg aggatcaggc ggacgtcctg gtcgaacagg ccgcccatgt
     3781 cgcagtcgag acgaaggcgg gtgctcctca tctcggcaag cggcaggtcc agccaccgct
     3841 tcagatggtc gtacaggcgc tgcagcgtcg agagccggag gaggagaccc cggatctcga
     3901 ggctgaagtc ggggggagcc tcggggcgtc cggagccgag ggattcgccg aagaactgac
     3961 cggacagggc gatgtcgcag gtcagcacgg catcggcgcc ggtcccttcg accgcgcgga
     4021 cgtgccacgt gaccctctcg cggccgctgc tcgaaagaag agcccacgat ccgctcatcc
     4081 tctttccgcg agataggaaa agcgggaaag ccgccgctcc gcagacagcc ggcggacgca
     4141 ttccaggaac cgctgcaggc cgtcgaaatt cgcctccccc tcgcgttcca ccagggcctc
     4201 ccgatgccgc tcccgcgcgg tccgccagcg ctcggcgatc cgctccgccg cttcggtgac
     4261 gtcttcggcc cccagcagcg tgaatcccgc ctggcggatc agccgctcgt tctcaccggg
     4321 gggaacgaac aggtagcggc cgatcgaggc ccgcatcgcg acctcctcgt gcgagaccat
     4381 cccggtgacg accagggcgt cggtgaacag catccgcccg cccgggcgca gcacccggtg
     4441 ccattcgccg agcacttcga gccgattcga gatgtggcac atcgcgtcgt tggagagcac
     4501 cgcgtcgaag gtggccgggg agaaggggag cggccggcgg gcgtccacca cctggaaggt
     4561 cacccgatcc gcgacaccgc gctccgccgc cagctgcgtg ccattgcgaa cgccgtgcgc
     4621 gttgatgtcc acgccggtga cgcgacagcc gcgcgcggcc gcgagatgca cggccgggcc
     4681 gccggagccg ctcccgacct ccagcacccg gctcccttcg cgcacctgcg cctggtccgc
     4741 ggatcgcagc cagtcctccg ccgtcatcca gctgctctgg ccgatgtcgt cgccgaacgc
     4801 ctcgcggcgc acctcggcct cggcgcggct gccgaagtcg gaatagatgt tgtcgtagag
     4861 atcgaccgcc ttggacatcg ttccaagcct actccgaata ctgcgtctcc tcggggacgt
     4921 cagtcgggct ttcgaattcg agcacgtacc cgtcgggatc ggtcacgctc gtgacccaca
     4981 tccggttgcc gacaaggggg cgcgaagccg ccaccccgcg cgcgaccacc tcgcggtaga
     5041 aggccggggc atcgtcgcag acgaagcaga tggagacacc cacgcccacc ttcccctccg
     5101 acttccagga gtcgtgcccc tcgcgcgcga actgctgcag catcacggcg gcatcgccgt
     5161 tccggagcca gcaccaccgc acgcggtcgt ccacgaccca gcggttcgtc atctcgaacc
     5221 cgagtccatc gacatagaag cggagggagg cctccatgtc cgcgacgcgg aagaagggga
     5281 ccgcttgccg gacgttcgct ccaggtttcg atgcgccgct cacgcgtcgt gcctcgctca
     5341 cggccccgcc tgcgccagat ggtgttccag gttgtcccag gcctcggtcg agctggtcgc
     5401 gacgccgtcg cagtcggtgt cccgctccgc ctgcgat
//
LOCUS       DASRIQ010000634         8011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_491962, whole genome shotgun sequence.
ACCESSION   DASRIQ010000634 DASRIQ010000000
VERSION     DASRIQ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491962"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFD06_15770"
     CDS             complement(<1..295)
                     /locus_tag="VFD06_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN05043.1"
                     /translation="MPAIVILAVAFWLFISVKILNEYERGVVFRLGRLRSEPVGPGIV
                     IVAWPIDRMVRVSQRVVVHDVPPQDVITSDNVSVKVNAVVYFRVVDPRNAIIQV"
     gene            complement(345..1712)
                     /locus_tag="VFD06_15775"
     CDS             complement(345..1712)
                     /locus_tag="VFD06_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HZN05044.1"
                     /translation="MTHRPTGGRRVAIAATAWLGLALGLAAAAAAAEPAHPAAASAQA
                     GSEALVYTVTLDDAIHPISSKYFVDALDRADKEGAALFILKLDTPGGLMTSMEEMISR
                     MTHARVPVVVFVHGSKAASAGFFLTIAADVAVMAPGTRFGAAHPVTIGAEIPKDSPIN
                     AKIENDAAAYARSLAENRRRNAVQAEKAVRESLAFTEQEALKLGLIDFVAKDEGEILK
                     RLDGRPIRRFSGESITLDLGRTRTVSLDMSGRERFLSFVANPTVAALLLLIGILGLYV
                     EFTHPGLIAPGLVGAICLLLFIFASQMLPINWIGVALVVLGVVLFLLEIKIVSHGALA
                     AGGVVSLVLGALILFPNSPEMPGWRAARMLVLGVAAAAGTIMAILTVLVTRVWKLRPT
                     TGASGLLEERGTALTDLNPEGRVFVHGEYWNARAARPVAKGAAVRVTGVRDLMLDVDE
                     DRERR"
     gene            complement(1709..2911)
                     /locus_tag="VFD06_15780"
     CDS             complement(1709..2911)
                     /locus_tag="VFD06_15780"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN05045.1"
                     /translation="MTAPLPPPLARRLEVIGVSPTLQVMMEAKRLRARGVDVVDFGPG
                     EPDFNTPENVKRAGQQAIAQDLSHYVETAGIPELRRAIVERYRRTYGASWDDADVVTG
                     VGGKNVLFLLALSLFDPGDRVAIYAPYWVSFPEQVRLCGAEPVILPCEETETGPIARA
                     SALEPHRGRLKAVILNSPCNPSGAVIPADEVAHFAAFAASSSTYLVSDETYDAFSYGP
                     EGFASFAAHKEALADRLVLVNSLSKAYAMTGWRVGYALAPRPIAEAMKKVQSHDATHT
                     AAVAQAAAREALSGAQDSVAAMREEYRRRRDELVPALNRLRGVRCPLPGGAFYVFPDV
                     RGAMETLGCSTSVEFAQRLMTEAAIAVVPGEAFGVPGRLRLSYALAPERMREGVVRLR
                     RLLGETSA"
     gene            complement(2908..3408)
                     /gene="coaD"
                     /locus_tag="VFD06_15785"
     CDS             complement(2908..3408)
                     /gene="coaD"
                     /locus_tag="VFD06_15785"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZN05046.1"
                     /translation="MQVTAIYPGTFDPITNGHLDIIERGARLFDRLIVALLRNAEKEP
                     LLPLAERQEIVRAVVARFPSVTVETFDGLLVDYCRARGARAIVRGLRALSDFEYEFQM
                     ALMNRRLEPGVETVFMMPSEAWSYVSSRLVKEVARLGGDVSGLVPKEALPRLLALAAA
                     SRETGR"
     gene            complement(3429..5171)
                     /gene="recN"
                     /locus_tag="VFD06_15790"
     CDS             complement(3429..5171)
                     /gene="recN"
                     /locus_tag="VFD06_15790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZN05047.1"
                     /translation="MLVVSAPASLMIRCLHVRNLAIVKEATLDLGPGLNLLTGETGAG
                     KSILVDALLLGLGGRAETDQVRTGEERAAVAIEFEVTSPEARTFLEEHGYPLEQGHVV
                     VQREVAAEGRGRAFIGGVPAPVADLKAFGALVVSIHGQHQHQTLLDPASHLPLLDREG
                     DVAEDLPAMTDAARELHESSLRLLSLRDGAQRLAQRIDLLTWQVQEIDAAAVQPEERA
                     ALRAERDRLRHAEQILRHGHEAFEALYGGEGAALARLAEGIRAAREVARYEAGLDADL
                     DRAEAARSEIEEVAFRLRDLMERLQPDPQRLQAADDRLQQIDALLRKYAANGDEAGLA
                     AWRAAAAEELRTLTDGGATVAELEAKMEGLRARALETAQRLSRRRRAAAAALERRVEK
                     ELEGLAMSGTRFAVDLRLRPGPGSGLWVEGEEVAVDATGYDVVEFLLSANRGEALRPL
                     HATASGGELSRIMLALETVRLGRSAARSLVFDEVDAGVGGAVAEVLGRKLRALAATHQ
                     VLCVTHLAPIASQADRHARVTKRAARGRTEAVVEPLDARGRVQEVARMLAGESVTASA
                     LKHAAEMLSKSAAR"
     gene            5306..6091
                     /locus_tag="VFD06_15795"
     CDS             5306..6091
                     /locus_tag="VFD06_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZN05048.1"
                     /translation="MAPSTFAPLAVAAAPGLHADPLRLIAESGWVAQGVLLALLIFSV
                     LSWAVMLKRWRYLRRAETESKRLVADLQQDKRLSEMYDRAARHTASPLAPILQAGFRE
                     LISAVNEGVGPGRGAGPATEEQKQRVLNRLRRRVEEVAGEQAETLDRNLGILATTGTV
                     APFIGLFGTVWGIMDAFQGIGLTGSASLAAVAPGISEALVTTAAGLAAAIPAVVGYNV
                     LLARARRLGSRIDRFVPQFLSQAEAQLDATRPAAAVEAGKFRI"
     gene            6129..6608
                     /locus_tag="VFD06_15800"
     CDS             6129..6608
                     /locus_tag="VFD06_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HZN05049.1"
                     /translation="MSVPGRGAGRFGPALSEINVTPLVDVILVLLIIFMVTAPMMSRG
                     IDVRLPRTESGSDTTEDRLIVSVDRDSTIYINDRPVNLALLTERLKSEMERTGSDFVF
                     LKADQDVSYGRVMAVMDQIKRAGADKVGMVTRHETPKPAAAKPKAPTTKGGARDKRP"
     gene            6635..7498
                     /locus_tag="VFD06_15805"
     CDS             6635..7498
                     /locus_tag="VFD06_15805"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZN05050.1"
                     /translation="MHGPAPAREVADGGIGGPVIVLSIVMHVLAFIFAVGVPRLLSSG
                     PSGNKVYVVDLVSLPGSPAPAAPAPPAAAAPAPEPPKPAPVKPPETKKPEVKKEPPKK
                     APVKPVVLPDKNVPKKPTKTPVKPPEKKPAEPEPTPVEAPEESGGEEEAAAAAPTATP
                     PATAKPGGAAGTGTGAGAGAGSGTGGGDEYDFYIALLQRRIEAAWKRPVSTSREIRTA
                     SVYFELSPTGRLLKLELKTPSGYAPFDRSIMQAVRDAEPFPAFPLALKMDRLTPTLQF
                     ELTPLAGDAPQ"
     gene            7528..>8011
                     /locus_tag="VFD06_15810"
     CDS             7528..>8011
                     /locus_tag="VFD06_15810"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05051.1"
                     /translation="MNLRPRPSRASGAAGLLVLSALPVALAALLAAGALAQEERPSDA
                     PIIKITGSAMSKVPIAIPRFDGGGTPKGDEAASTLYEVLRDDLLFSNYFKLVPDEYLK
                     LVQPFAGRKTDYKEWQGIGADAVLVGTSRPEAAELTFEARVYDMGEQRLALGKKYRAN
                     T"
BASE COUNT         1173 a         2970 c         2781 g         1087 t
ORIGIN      
        1 ccacctgaat gatcgcgttc ctcggatcga ccacccggaa gtagaccacc gcgttcacct
       61 tcaccgagac gttgtcggag gtgatgacgt cctgcggcgg cacgtcgtgc accacgaccc
      121 gctgcgacac ccgcaccatc cggtcgatcg gccacgccac gatcacgatc cccgggccga
      181 ccggctccga ccgcaggcgg cccagccgga agacgacgcc gcgctcgtac tcgttgagga
      241 tcttgacgct gatgaagagc cagaaggcaa ccgccaggat gacgatcgcc ggaatgcccc
      301 cgaactggat cacacacccc tccttgcggc cggctctgcg gcggtcatcg ccgctcccgg
      361 tcctcgtcga cgtccagcat caggtcgcgg acgccggtca cccggaccgc cgcccccttc
      421 gccaccggcc gcgccgcgcg ggcgttccaa tactcgccgt gcacgaagac gcgcccttcc
      481 ggattgagat ccgtgagggc ggtgccgcgc tcctccagaa ggccgctcgc gccggtggtc
      541 ggccggagct tccagacacg ggtgaccagg accgtgagga tggccatgat cgtcccggcg
      601 gcggcggcga ctcccaggac gagcatgcgg gcggcgcgcc agccgggcat ctccggcgag
      661 ttcgggaaga ggatgagggc gccgagcacc aggctgacga cgccgccggc agcgagcgcg
      721 ccgtggctga cgatcttgat ttcgagcagg aacaggacga cgccgaggac cacgagcgcc
      781 acgccgatcc agttgatcgg cagcatctgg ctggcgaaga tgaacagcag caggcagatg
      841 gcgccgacga gcccgggcgc gatgagcccc ggatgcgtga actcgacgta caggcccagg
      901 atgccgatca acagcagcag ggccgcgacg gtcgggttgg ccacgaagct caggaaccgc
      961 tcgcgcccgc tcatgtcgag ggaaacggtg cgcgtgcggc cgagatcgag agtgatgctc
     1021 tcgccggaga accggcggat cggccggccg tcgaggcgct tgaggatctc cccctcgtct
     1081 ttcgcgacga aatcgatgag cccgagcttc agcgcctcct gctctgtgaa ggccaggctc
     1141 tcccgcaccg ccttctccgc ctggacggcg ttgcgccggc ggttctcggc cagcgagcgg
     1201 gcgtaggcgg ccgcgtcgtt ctcgatcttc gcattgatcg gcgagtcctt cgggatctcc
     1261 gcaccgatgg tcaccggatg cgccgccccg aaacgggtcc ccggcgccat gaccgccacg
     1321 tcggcggcga tggtcaggaa gaacccggcg gaggccgcct tcgatccgtg cacgaagacg
     1381 accaccggga cgcgcgcgtg cgtcatgcgg gagatcatct cctccatcga ggtcatcaga
     1441 ccgcccgggg tgtcgagctt caggatgaag agcgccgccc cctccttgtc ggcgcgatcg
     1501 agggcgtcca cgaagtactt ggaggagatc ggatggatgg cgtcgtcgag cgtcaccgtg
     1561 tagacgagcg cctcggagcc ggcctgcgcc gacgcggccg ccggatgcgc cggctccgcc
     1621 gcggccgcgg ccgcggcgag tccgagcgcg aggccgagcc acgccgtcgc cgcaattgcc
     1681 acgcgccggc cgccggtggg gcggtgggtc acgcggaggt ctctccgagg agtcggcgca
     1741 gccgcaccac cccttcgcgc atccgctccg gggccagcgc gtacgacagg cgcagccggc
     1801 cgggcacgcc gaacgcctcg cccggcacga cggcgatcgc cgcctcggtc atcaggcgct
     1861 gcgcgaactc caccgacgtg gagcagccga gggtctccat cgcgccgcgc acgtccggga
     1921 agacgtagaa cgcgccgccg gggagcgggc agcggacgcc gcgcagccgg ttcagcgccg
     1981 gcaccagctc gtcccggcgg cggcggtact cctcccgcat ggcggcgacc gagtcctgcg
     2041 ccccggacag cgcctcgcgc gccgccgcct gcgccacagc ggcggtgtgg gtcgcgtcgt
     2101 gcgactgcac cttcttcatc gcctcggcga tcggccgcgg cgccagcgcg tagccgacgc
     2161 gccacccggt catggcgtag gccttcgaca gcgagttgac cagcaccagc cggtccgcca
     2221 gcgcctcctt gtgcgccgcg aacgaggcga acccctcggg gccgtaggag aaggcgtcgt
     2281 aggtctcgtc gctgacgaga taggtggagc tcgatgcggc gaaggcggcg aagtgtgcca
     2341 cctcgtcggc cgggatgacc gcgccgctcg ggttgcacgg cgagttcagg atcaccgcct
     2401 tcagccggcc gcggtgcggc tccagggcgc tggcgcgcgc gatcggaccg gtctcggtct
     2461 cctcgcacgg caggatcacc ggttcggcgc cgcacagacg cacctgctcg ggaaacgaca
     2521 cccagtacgg cgcgtagatc gccacccggt caccgggatc gaagagcgac agcgccagca
     2581 ggaagaggac gttcttgccg ccgacgccgg tgaccacgtc ggcgtcgtcc caggaggcgc
     2641 cgtaggtgcg gcggtagcgc tcgacgatgg cccgccgcag ctcggggatc ccggcggtct
     2701 cgacgtagtg gctgaggtcc tgtgcgatcg cctgctggcc ggcccgtttg acgttctccg
     2761 gggtgttgaa gtcgggctcg ccggggccga aatcgacgac gtcgacgccg cgggcgcgga
     2821 ggcgcttcgc ctccatcatc acctggaggg tcggcgagac gccgatcacc tcgaggcgcc
     2881 gcgccagcgg gggtggcagc ggcgcggtca tcgccccgtc tcccgcgacg cggcggcgag
     2941 cgccagcagg cgcggcagcg cctccttggg gacgagcccg gagacgtcgc cccccagacg
     3001 cgccacctcc ttcaccaggc ggctgctgac gtacgaccac gcctcgctcg gcatcatgaa
     3061 gaccgtctcc acgcccggct cgagccggcg gttcatcagc gccatctgga actcgtactc
     3121 gaaatcggag agggcccgga gcccccgcac gatggcccgc gcgccgcgcg cccggcagta
     3181 atcgacgagc aggccgtcga aggtctcgac cgtgacgctg ggaaagcggg cgacgaccgc
     3241 ccggacgatc tcctgccgct cggcgagcgg caggagcggc tccttctcgg cgttgcgcag
     3301 gagcgccacg atgaggcggt cgaagagacg cgcgccgcgc tcgatgatgt cgaggtgccc
     3361 gttggtgatc gggtcgaagg ttcccgggta gatcgcagtg acctgcatcg acgtcgctgg
     3421 cagctcccct agcgggccgc gctcttcgac agcatctcgg ccgcgtgctt cagcgccgag
     3481 gcggtgaccg actccccggc cagcatacgc gccacctcct gcacccggcc gcgcgcgtcg
     3541 agcggctcga ccaccgcctc cgtccgcccg cgcgccgccc gcttcgtcac ccgcgcgtgc
     3601 cggtcggcct gcgaggcgat cggcgccagg tgcgtcacgc acagcacctg gtgcgtcgcg
     3661 gcgagcgcgc gcagctttcg tccgagcacc tccgccaccg ccccgcccac ccccgcgtcg
     3721 acctcgtcga acacgagact gcgcgcggcc gatcgtccga gccggaccgt ctcgagcgcg
     3781 agcatgatcc gcgacaactc cccacccgag gccgtcgcgt gaagcggccg cagcgcttcg
     3841 ccgcgattcg cggacaggag gaattccacc acgtcgtatc cggtggcgtc aacggccact
     3901 tcctcccctt cgacccacag gccgctgccg ggaccgggtc ggaggcgcag atcgacggcg
     3961 aagcgggtgc ccgacatcgc cagcccttcg agctctttct ccacgcggcg ctcgagcgcc
     4021 gccgccgcgg cgcgccggcg gcgcgacagc cgctgcgccg tctcgagggc gcgggccctg
     4081 agaccctcca tcttcgcctc cagctcggcg acggtggcgc cgccgtcggt gagcgtgcgc
     4141 agctcctcgg cggcggcggc gcgccaggcg gcgaggccgg cctcgtcgcc gttcgcggca
     4201 tatttgcgaa ggagcgcgtc gatctgctgc agccggtcgt ccgccgcctg cagccgctgt
     4261 gggtccggct gcagccgctc catgagatcg cgcagccgga acgccacctc ttcgatctcc
     4321 gatcgcgccg cctcggcgcg gtcgaggtcg gcgtcgagcc ccgcctcgta acgggccacc
     4381 tcgcgcgccg cgcgaatccc ctccgccagc cgcgccagcg ccgccccctc ccctccgtag
     4441 agggcttcga acgcctcgtg gccgtgccgc aggatctgct cggcgtgccg cagccggtcc
     4501 cgctccgccc gcaacgcggc ccgctcctcc ggctgcaccg cggcggcgtc gatctcctgc
     4561 acctgccagg tcaggagatc gatgcgctgc gccaggcgct gcgcgccgtc ccgcagcgac
     4621 agaagccgca ggctcgattc gtgaagctcg cgcgccgcgt ccgtcatcgc cggcaggtcc
     4681 tcggcgacgt ctccttcccg gtcgagcagc ggcaggtgcg aggccggatc gagcagagtc
     4741 tggtgctggt gctgtccgtg gatggagacg acgagcgcgc cgaacgcctt gagatccgcc
     4801 accggcgccg ggacgccgcc gatgaaggcg cggccgcgtc cttcggcggc gacctcgcgc
     4861 tgcaccacga cgtgcccctg ctccagcggg tagccgtgct cctcgaggaa ggtgcgcgcc
     4921 tcgggggagg tgacctcgaa ctcgatggcg accgcggcgc gctcctcgcc ggtgcgcacc
     4981 tgatcggtct ccgcccggcc gccgaggccc aggagcaggg cgtccaccag gatcgacttg
     5041 ccggcgccgg tctcgccggt gaggaggttc aggcccggtc cgagatcgag cgtcgcctct
     5101 ttgacgatcg cgaggttcct gacgtgcagg cagcggatca tgagcgacgc cggcgccgag
     5161 actaccagca cggatctttc gttgtcaatg aaacgggcgt gtgctaactt acggccgctt
     5221 gcgcttcgcg ccgcgtccgc gcatattttc catccgagga tgcgcggatc gatccggcgc
     5281 ccctgacagg agacctcacg cgcgcatggc cccatcgacg ttcgcgcccc tcgccgtagc
     5341 ggcagccccc ggactccacg ccgatcccct ccggctgatc gccgaatccg gatgggtggc
     5401 gcagggagtg ctcctggcgc tgctcatctt ctccgttctc tcctgggcgg tcatgctcaa
     5461 gcgctggcgg tacctgcgtc gcgccgaaac cgagtcgaag cggctggtcg ccgacctcca
     5521 gcaggacaag cgcctctccg agatgtacga ccgggcggcg cggcacaccg cgagcccgct
     5581 cgcgccgatc ctccaggccg gcttccgtga gctgatctcc gcggtcaacg agggggtcgg
     5641 cccggggcgc ggcgccggtc ccgcgaccga agagcagaag cagcgcgtcc tgaaccggct
     5701 gcggcgccgg gtggaggagg tggccgggga gcaggcggag accctcgatc gcaacctcgg
     5761 gatcctggcc accaccggca cggtcgcgcc gttcatcggc ctgttcggga cggtgtgggg
     5821 gatcatggac gccttccagg ggatcggcct caccggctcg gccagcctcg ccgcggtcgc
     5881 ccccgggatc tccgaggcgc tggtgaccac cgcggccggc ctcgccgccg ccatccccgc
     5941 cgtcgtcggc tacaacgtcc tcctggcgcg cgcccgccgt ctcgggtcgc gcatcgatcg
     6001 cttcgtgccg cagttcctct cccaggcgga agcgcagctc gacgccaccc gccccgccgc
     6061 cgcggtggag gccgggaaat tccggatctg agcatgggcg gcgaccgccg tggggggaac
     6121 gcatggcgat gagcgttccc gggcgcggcg ccggccgctt cggcccggcc ctttccgaga
     6181 tcaacgtcac tccgctcgtc gacgtcatcc tcgtcctgct catcatcttc atggtgaccg
     6241 ccccgatgat gagccgcggc atcgacgtcc gcctgccgcg cacggaatcg ggcagcgaca
     6301 cgaccgagga tcgcctcatc gtcagcgtgg atcgtgacag caccatctac atcaacgacc
     6361 ggccggtgaa cctggcgctc ctgaccgagc gcctcaagtc ggagatggag cggaccggca
     6421 gcgacttcgt cttcctgaag gccgatcagg acgtgtcgta cggccgggtg atggcggtga
     6481 tggatcagat caagcgggcc ggcgccgaca aggtcggtat ggtgacccgc cacgagacgc
     6541 cgaagccggc cgcggcgaag cccaaggcgc cgacgacgaa gggcggcgcg cgggacaagc
     6601 gcccgtgaag ccgctcacct tgggaggcgg gttgatgcac ggacccgccc ccgcccggga
     6661 ggtcgccgac ggcggcatcg gggggccggt catcgttctc tcgatcgtca tgcacgtcct
     6721 ggcgttcatc ttcgccgtcg gcgtgccgcg cctcctctcc tcgggaccgt cggggaacaa
     6781 ggtctacgtc gtcgatctcg tgagcctgcc cggcagccct gcgccggccg ctccggcccc
     6841 gccggccgcg gccgcgcccg cgcccgagcc gcccaagccg gcccccgtca agccgccgga
     6901 gacgaaaaag ccggaggtga agaaggagcc gccgaagaag gcgccggtca agccggtcgt
     6961 cctgccggac aagaacgtcc cgaagaagcc gaccaagact cccgtcaagc cgcccgagaa
     7021 gaagcccgcc gaaccggagc cgacgccggt cgaggcgccc gaggagtcgg gaggcgagga
     7081 ggaggccgcc gcggccgctc cgaccgccac cccgccggcc acggcgaagc cgggaggcgc
     7141 ggccggaacc gggaccggcg ccggggcggg ggccggcagc ggcaccggcg gcggcgacga
     7201 atacgacttc tacatcgctc tgctgcagcg ccggatcgag gcggcgtgga agcggccggt
     7261 gtccacctcg cgcgagatcc gcacggcctc cgtgtatttc gagctgagcc cgactggccg
     7321 cctgctcaag ctcgagctga agaccccgag cggctacgcg ccgttcgatc gctccatcat
     7381 gcaggcggtg cgggacgccg aaccgttccc ggcgttcccg ctcgcgctga agatggacag
     7441 gttgacgccg accctgcaat tcgagctgac ccccctggcg ggggacgctc cccagtgaat
     7501 cgcccgactc tcccggagac cgatcccgtg aaccttcgtc cgcgtccctc ccgcgcgtcc
     7561 ggcgccgcgg ggctgctcgt cctgtccgcc ctcccggtcg cgctcgccgc cctcctcgcg
     7621 gcaggggccc tcgcccagga ggagcgcccg tccgatgccc ccatcatcaa gatcaccggc
     7681 agcgcgatgt cgaaggtccc gatcgccatt ccgcgcttcg acggcggcgg cactccgaaa
     7741 ggggatgagg cggcgtcgac gctgtacgag gtgctgcgcg acgatctgct cttctccaac
     7801 tacttcaagc tggttcccga cgagtacctc aagctcgtcc agccgttcgc gggccgcaag
     7861 accgactaca aggagtggca ggggatcggc gccgacgccg tgctcgtggg gacgtcccgc
     7921 ccggaggcgg ccgagctgac gttcgaggcg cgcgtctacg acatggggga gcagcgcctg
     7981 gcgctcggca agaaataccg ggccaacacc g
//
LOCUS       DASRIQ010000635         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2073497, whole genome shotgun sequence.
ACCESSION   DASRIQ010000635 DASRIQ010000000
VERSION     DASRIQ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073497"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFD06_15815"
     CDS             <1..573
                     /locus_tag="VFD06_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05052.1"
                     /translation="NPVRRAAQVEMLASVLGIEDRLVLQELKEAVRDRRREVGGRAAS
                     ALRAQKPGSEAEALLVRALLDDGAVRAALLPEVEEADLASSRLLEIVRAVRRLSDAGE
                     DVTYPRVASEIGDAAREALTSIAATTPPPATVDGGRGCLRALRAERLRQETKELTKRI
                     MKASGEEYSALALRKLALQKQIEALGGVSV"
     gene            589..2286
                     /gene="rpoD"
                     /locus_tag="VFD06_15820"
     CDS             589..2286
                     /gene="rpoD"
                     /locus_tag="VFD06_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZN05053.1"
                     /translation="MGIEEKYEEVRHLINLGRERGYLLYDEVNDVLPEEMHTPEDLDD
                     MFLLFDSLGIEMVDSEEKYKQKVEEKAAAGEKGEAAEEPGDQKVDLTPGALEKTNDPV
                     RMYLREMGTVPLLTREGEVEIARRIERGEKNVVKALARSKYVVSQMLDYAQRIRNKQI
                     DVESLITVSEEEEEHADRKRRQILDAMNKVARLARKLEDQEKHLSRLTEGSRRWKEVR
                     FDSARSRVGIGRVIFEMGLVTGKIKELAKKVKDTASKLEDLDRDIKYYEKRLKILKDP
                     KHRKDVRDKVHSAHEEIARIAREMDTTSEEILRTADGIRAGEKMSGKAKTELVEANLR
                     LVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAI
                     ADQARTIRIPVHMIETINKLIRTSRALVQEIGREPTPEEIAKKMGIPVMKVRKVLKIA
                     QEPISLETPIGEEEDSHLGDFIEDRGVTSPVDAVIQLNLKEQTSKVLKTLSPREEMVL
                     KMRFGVGDGSEHTLEEVGQSFAVTRERIRQIESKALRKLRHPSRSQRLRAFLDGTGG"
BASE COUNT          493 a          775 c          938 g          350 t
ORIGIN      
        1 aacccggtgc gccgggcggc gcaggtggaa atgctggcgt cggtcctggg gatcgaggac
       61 cggctggtcc tgcaggagtt gaaagaggcg gtgcgcgacc ggcgccgcga ggtcggcggc
      121 cgggcggcat ccgccttgcg agcgcagaag ccggggagcg aagccgaggc gttgctggta
      181 cgggcgctcc tggacgatgg ggcggtgcgg gccgccctcc tcccggaggt ggaggaggcg
      241 gacctggcct cttcgcggct gctggagatc gtcagggcgg ttcggaggct gtcggacgcg
      301 ggggaggacg tgacgtaccc gcgggtggca tcggagatcg gcgatgcggc gcgggaggcg
      361 ctgaccagca tcgccgcgac gacgcccccg ccggcgacgg tggatggggg gcgcgggtgc
      421 ctgcgggcgc tgcgcgcgga gcggctcagg caggagacga aggaactgac gaagcgaatc
      481 atgaaagcga gcggagagga gtactcggca ctggcgctcc gcaagctcgc gttgcagaag
      541 cagatcgagg cgctcggcgg cgtgtcggtc tgatataggg aggccccctt gggcatcgag
      601 gaaaaatacg aggaagtccg gcacctgatc aatctcggtc gggagcgcgg ctacctgctc
      661 tacgacgagg tgaacgacgt cctgccggag gagatgcaca ctcccgagga tctggacgac
      721 atgttcctgc tcttcgactc cctcgggatc gagatggtcg attccgagga gaagtacaag
      781 cagaaggtcg aggagaaggc ggccgccggg gagaagggcg aggcggccga ggagccgggg
      841 gaccagaagg tcgacctgac tcccggggcg ctcgagaaga ccaacgaccc ggtgcgcatg
      901 tacctgcgcg agatggggac ggtcccgctc ctgacccgcg agggggaggt cgagatcgcc
      961 cggcgcatcg agcgcggcga gaagaacgtc gtcaaggcgc tggcgcgcag caagtacgtc
     1021 gtcagccaga tgctcgacta cgcccagcgc atccgcaaca agcagatcga cgtcgagagc
     1081 ctgatcacgg tgagcgagga ggaggaggag cacgccgatc gcaagcgtcg gcagatcctc
     1141 gacgccatga acaaggtggc gcgcctcgcc cgcaagctcg aggaccagga gaagcacctg
     1201 tcccggctca ccgaggggtc gcggcgctgg aaggaagtcc gcttcgacag cgcccgcagc
     1261 cgcgtcggta tcggccgggt gatcttcgag atgggtctgg tcaccggcaa gatcaaggag
     1321 ctggcgaaga aggtcaagga cacggcgtcg aagctcgagg acctcgaccg cgacatcaag
     1381 tactacgaga agcggctgaa gatcctcaag gacccgaagc accgcaagga cgtgcgggac
     1441 aaggtccact cggcgcacga ggagatcgcc cgcatcgcgc gggagatgga cacgacgtcc
     1501 gaggagatcc tgcgcacggc ggacgggatc cgcgccgggg agaagatgtc cggcaaggcc
     1561 aagaccgagc tggtcgaggc caacctccgc ctggtggtgt cgatcgccaa gaagtacacc
     1621 aaccgcgggc tgcagttcct cgacctgatc caggagggga acatcggcct gatgaaggcg
     1681 gtggacaagt tcgagtacaa gcgcggctac aagttctcga cctacgcgac ctggtggatc
     1741 cgccaggcga tcacccgcgc catcgccgac caggcccgca ccatccgcat cccggtgcac
     1801 atgatcgaga cgatcaacaa gctcatccgc acctcgcgcg cgctggtgca ggagatcggc
     1861 cgcgagccga cgccggagga gatcgccaag aagatgggca tcccggtgat gaaggtgcgc
     1921 aaggtgctca agatcgcgca ggagccgatt tcactcgaga cgccgatcgg ggaagaggag
     1981 gattcccatc tcggggattt tatcgaagat cgcggcgtga cgtcgccggt cgacgcggtc
     2041 atccagctga atctcaagga gcagacctcc aaggttctca agaccctctc accccgagag
     2101 gagatggtgc tcaaaatgcg tttcggggtc ggcgacggaa gtgaacacac cctcgaggag
     2161 gtcggacagt cgttcgcggt gacccgcgag cggatccggc agatcgaaag caaggcgctc
     2221 cgcaagctgc gtcatccctc gcgcagccag cgcctccggg cctttctcga cgggaccggc
     2281 gggtagccac gactgacaga gcggtcatga cggactgaaa gggcggggcc ggaaggctcc
     2341 gccctttttt tgtccgcggc cgcggggggc cggcccgccc ttgacggccc gggccccggc
     2401 cctatcttga gccgcggggg actccgtgtt cgccttccgc gccgcgctca tcgccctgct
     2461 cgcctggtgg accgcccgtc tcggatcggg ggcgcagcag gggtgcttcc tcgacctggt
     2521 gaatctggcg ttccacgagg cggggcatct cttcct
//
LOCUS       DASRIQ010000636         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2594498, whole genome shotgun sequence.
ACCESSION   DASRIQ010000636 DASRIQ010000000
VERSION     DASRIQ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2594498"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFD06_15825"
     CDS             <1..990
                     /locus_tag="VFD06_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN05054.1"
                     /translation="ADFTHLPVRCGFIALMPQWDFLEFLARHAKMFPGFRLCMETEAV
                     DLIEEAGVVKGVRTQGPSGPLSIRAALVVGADGRHSTVRERAGLLVETLGAPMDVLWF
                     RLSRAPGDPGQTMGRFEAGRIFVMINHGTHWQIGYVIPKGSAERLRAAGLDRFRDTVA
                     ALLPGGGERVAEIRAWDDVKLLTVVVDRLRRWFRPGLLCIGDAAHAMSPVGGVGINLA
                     IQDAVAAANLLAAPLREGTLGTRDLERVERRRRWPTRVTQRVQVFLQEQVIARTLAAP
                     GGGAASPPLPLPLRLLKRSPLLRRLPGRLIGLGVRPEHVRTPALAPAPPPAGT"
     gene            complement(1016..2167)
                     /locus_tag="VFD06_15830"
     CDS             complement(1016..2167)
                     /locus_tag="VFD06_15830"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HZN05055.1"
                     /translation="MFRMSRASFLLAALLALAPATPAGAQDVTLPPSGDNQKAAVTQF
                     MGIASVTVEYSSPDVHAPDGTDRKGKIWGTLVPYGYAEDNFGTCGKKCPWRGGANENT
                     VVRFSHPVTVEGKPLAAGAYGLHFLAGEKDWTVIFSKNSTSWGSYFYNEAEDALRVTV
                     KPAAAPYTEWLTYEFVDRQPASTVLVLQWEDLRVPIAIALPNDTEIYYQTLKRELQDT
                     PGFDYHGWQQAADYLIEKKVHLDEAERWAQAAVSMPFIGEENFATLRTLASAQRARGK
                     EAEAKATITKALAHPTASPTDIYQYARPMIREGKPQEALVVFQSASKRMGAKWPLDLG
                     IARSYSALGDYKAALKYAKASLTVAPDQVNKDNVQRMIAKLEKGEDMNK"
     gene            complement(2339..3268)
                     /locus_tag="VFD06_15835"
     CDS             complement(2339..3268)
                     /locus_tag="VFD06_15835"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HZN05056.1"
                     /translation="MQSARWMAGLAVVAWLVTGQAGAGPHDAGRHHAPPPAGVELKEA
                     EVIVPMDSFGGRPVVSLTLDGKGPFRFVLDSGAGGSVVSRTLTDSLGLAAEGEVRVGS
                     PGGATNPGALVKIGRVEMGGAALVGVTMVATDLSRVFTLPEHPVGVLSAMSFSGHLVT
                     FDYPAKQIVIKPGALPAANGTDIFAYQAAQHIPTLSIDVAGMKVDAHVDSGSGRGLML
                     PGSLAAKLPLGGPLEELAKAKTVDGEWPIRRAPLDGQVRIGRFTLDKPQVDFMEGAPI
                     GNIGYEILKDYRLTVDRKNLRFRLEPGTTISRG"
     gene            3364..>3649
                     /locus_tag="VFD06_15840"
     CDS             3364..>3649
                     /locus_tag="VFD06_15840"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN05057.1"
                     /translation="MPRRPLTELEGCVLGVLSTLGPCTAYVVRCHFLESPSPYWSGSA
                     GSIYPLLKRLRGLGLVQERPREEGRRRSILLSLTAAGRTALAGWLLPPLPE"
BASE COUNT          484 a         1311 c         1272 g          582 t
ORIGIN      
        1 gccgacttca cccacctgcc ggtccgctgc ggcttcatcg ccctcatgcc gcagtgggac
       61 ttcctggaat tcctggcgcg tcacgcgaag atgttccccg gcttcaggct gtgcatggag
      121 accgaggcgg tcgatctcat cgaggaggcg ggggtcgtca agggggtgcg gacgcagggg
      181 ccctccggcc ccctctcgat ccgcgccgcc ctcgtcgtgg gcgccgacgg ccgccactcg
      241 accgtgcgcg agcgggcggg gctgctggtc gagacgctcg gagcaccgat ggacgtcctg
      301 tggttccggc tgtcccgcgc tcccggcgat cccggccaga cgatggggcg cttcgaagcc
      361 ggccggatct tcgtcatgat caaccacggg acgcactggc agatcggcta cgtcatcccg
      421 aagggatcgg ccgaacggct gcgcgccgcc ggcctcgacc gcttccgcga caccgtcgcc
      481 gccctcctcc cgggaggggg cgaaagggtg gccgagatcc gcgcctggga cgacgtgaaa
      541 ttgctgaccg tcgtggtgga tcgcctgcgt cgctggttcc gtccggggct gctctgcatc
      601 ggcgacgcgg cgcacgcgat gtccccggtc ggcggggtcg ggatcaatct cgccatccag
      661 gacgcggtgg cggccgccaa cctgctggcc gcgcctctcc gcgagggcac cctcgggacc
      721 cgtgacctcg agcgcgtcga gcgccgccgg cgctggccga cgcgcgtcac ccagcgggtg
      781 caggtctttc tgcaagagca ggtgatcgcc cggaccctcg cggctccggg cggtggcgcg
      841 gcatcgccgc cgctgccgct tccgctgcgc ctcctgaagc gctctcctct gctgcgccgg
      901 ctgccggggc gcctcatcgg gctcggcgtc cggcccgagc acgtccggac gcccgccttg
      961 gcccccgccc cgccgccggc cggaacctag cccggcagcg gggcgagatg ccggactact
     1021 tgttcatgtc ctcgcccttc tcgagcttcg cgatcatccg ctggacgttg tccttgttga
     1081 cctgatcggg ggcgacggtg agcgacgcct tggcgtactt cagcgccgcc ttgtagtcgc
     1141 cgagcgcgga gtacgagcgg gcgatgccga gatcgagcgg ccacttcgcg cccatccgct
     1201 tggaggcgga ctggaagacg accagcgcct cctgcggctt cccttcccgg atcatcggcc
     1261 gggcgtactg gtagatgtcg gtcggggacg cggtcgggtg cgcgagcgcc ttggtgatcg
     1321 tcgccttcgc ctccgcctcc ttgccgcgcg cccgctgggc gctcgcgagc gtccggaggg
     1381 tggcgaaatt ctcctcgccg atgaacggca tgctgaccgc cgcctgcgcc cagcgctccg
     1441 cctcgtcgag gtgcaccttc ttctcgatca ggtagtcggc cgcctgctgc cagccgtggt
     1501 agtcgaaccc gggggtatcc tgaagttcgc gcttgagcgt ctggtagtag atctcggtgt
     1561 cgttcggcag cgcgatggcg atcggcaccc ggaggtcctc ccactggagc accagcacgg
     1621 tgctcgccgg ctgacggtcg acgaactcgt aggtgagcca ctcggtgtac ggcgcggccg
     1681 ccggcttcac cgtgacccgc agggcgtcct ccgcctcgtt gtagaagtac gacccccacg
     1741 acgtggagtt cttcgagaag atgaccgtcc agtccttctc gccggcgagg aagtgcagcc
     1801 cgtaggcgcc ggctgcgagc ggcttccctt ccacggtcac cgggtgcgag aagcggacga
     1861 cggtgttctc gttcgcgccg ccgcgccacg ggcacttctt gccgcaggtg ccgaagttgt
     1921 cctcggcgta gccgtacggg acgagcgtgc cccagatctt ccccttgcgg tcggtgccgt
     1981 ccggggcgtg cacgtcgggg ctggagtact cgacggtgac cgaggcgatc cccatgaact
     2041 gggtgaccgc ggccttctgg ttgtcgccgc tcggcggcag ggtcacgtcc tgggcccctg
     2101 ccggagtggc cggagcgaga gcgagcaggg ccgcgaggag gaacgatgcg cgggacatgc
     2161 ggaacatgga tgctcctgtc gaatgggcgg cggaagacgc gcagacttcg caggccggac
     2221 cacggcacat gcctcgagcc ggggcggccg ccgattccgc acgcacaacc ggcgggcccg
     2281 gactccctgc gcgttgacga ttcagggcct gattctagga cggtccgggg cgtccgcgtc
     2341 aaccccgcga gatcgtggtg cccggctcca gccggaagcg caggttcttg cggtccacgg
     2401 tgagccggta gtccttgagg atctcgtacc cgatgttgcc gatcggcgcc ccttccatga
     2461 agtcgacctg gggcttgtcg agggtgaacc ggccgatgcg gacctggccg tcgagcggcg
     2521 cccggcggat cggccactcg ccatccaccg tcttcgcctt ggccagctcc tcgagcggtc
     2581 cgccgagcgg cagcttcgcc gccagggatc ccggcagcat cagcccgcgt ccggagccgg
     2641 agtcgacgtg cgcgtccacc ttcatcccgg cgacgtcgat cgaaagcgtc gggatgtgct
     2701 gcgccgcctg gtaggcgaag atgtcggttc cgttggcggc gggcagcgcc cccggcttga
     2761 tgacgatctg cttcgccggg tagtcgaagg tgacgaggtg gcccgagaag gacatggcgc
     2821 tgagaacgcc gaccgggtgc tcggggagcg tgaagacacg cgacaggtcg gtcgcgacca
     2881 tggtcacgcc gacgagcgcc gccccgccca tctcgaccct gccgatcttc accagcgctc
     2941 ccggattggt ggcgccgccc ggggagccga cccgaacctc cccttccgcc gcgagcccga
     3001 gcgaatcggt cagcgtccgg ctgaccacgc tcccccccgc gccgctgtcg agcacgaagc
     3061 ggaaggggcc cttgccatcg agcgtcagcg aaacgaccgg acgaccgccg aacgaatcca
     3121 tggggacgat cacttccgcc tccttcaact cgacgcccgc cggcggaggg gcatggtgcc
     3181 tccccgcgtc gtgcggcccc gcaccggcct gccccgtcac cagccacgcc acgactgcca
     3241 gacccgccat ccaccgcgct gactgcatgc gcctctcctt ccgatcccgc gttcgccggc
     3301 gcggagtata gtacgattcg tactatccgg ccagcgcccg cctgcgcggc cggggaggga
     3361 tcgatgccgc gacgtccgct gaccgagctg gagggatgcg tgctgggagt cctgtccacc
     3421 ctcggcccgt gcaccgcgta cgtcgtccgc tgccatttcc tggaatcgcc gagcccgtac
     3481 tggagcggca gcgccgggtc gatctacccg ctcctgaagc ggctgagggg cctggggctc
     3541 gtgcaggagc gcccgcgcga ggaagggcgc cggcgcagca tcctgctgag cctcaccgcc
     3601 gcggggcgga ctgcgctcgc gggctggctc ctccctcccc tgccggaag
//
LOCUS       DASRIQ010000637         4830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2334049, whole genome shotgun sequence.
ACCESSION   DASRIQ010000637 DASRIQ010000000
VERSION     DASRIQ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2334049"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /gene="rplT"
                     /locus_tag="VFD06_15845"
     CDS             complement(<1..296)
                     /gene="rplT"
                     /locus_tag="VFD06_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002343687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZN05058.1"
                     /translation="MPRVKRGTKRRQRRKKTLKLAKGFYIGKSKLYRIAKQAVEKSLG
                     YAYRDRRQRKRQFRTLWTVLINAAARLNDLSYSRFMEGLKKAGVDLDRKMLAELA"
     gene            complement(306..509)
                     /gene="rpmI"
                     /locus_tag="VFD06_15850"
     CDS             complement(306..509)
                     /gene="rpmI"
                     /locus_tag="VFD06_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016999300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZN05059.1"
                     /translation="MPKQKTKRGAAKRFRMSARGKLKRSKAYKRHILTSKSTKRKRQL
                     DMDTLVSKSDVRRVKSMLPYGRP"
     gene            complement(522..1091)
                     /gene="infC"
                     /locus_tag="VFD06_15855"
     CDS             complement(522..1091)
                     /gene="infC"
                     /locus_tag="VFD06_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZN05060.1"
                     /translation="MEKKLRINEKIRTREVRVIADNGEQLGIMPPEQALRMAMEAGLD
                     LVEVSPDAVPPVCRILDYGKYRYQLNKKAQDAKKRQRIIQVKEVKFRPKTDEHDYEFK
                     RNNMIRFLSKGKKVKATVIFRGRENMHRDIGHKILRRLRQEVEEFGLVESEPRQEGPF
                     LYMILAPKKWSGGAPPPGPAPASAPAPKR"
     gene            complement(1109..3043)
                     /gene="thrS"
                     /locus_tag="VFD06_15860"
     CDS             complement(1109..3043)
                     /gene="thrS"
                     /locus_tag="VFD06_15860"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZN05061.1"
                     /translation="MDPIQVTFPDGSVRAFPRGTTFEDLAHAWDPKIAKRMIAASENG
                     TARDVYLPLRHDAKVDFITPDSPAALEIYRHTTSHLLAHAVKELFPEVKIGIGPATEE
                     GFFYDFERPDPFTPEDLERIEARMREIKGRDFPIRRLEQPKAEALEYFEGLGDNLKVE
                     LVREKGGDVVSCYRQDSFMDFCTGPHVPSTGRLGAFKLLSIAGAYWKGSEENQQLQRI
                     YGTAFATEEQLAKHLAMLEEAKKRDHRRLGPALNLFSIEETAGPGLIFWHPHGTVLRQ
                     VLEDYWKEEHRRRGYQLVTTPHIAKDELWRRSGHLDYYKDNMYTFPIDEMGYVLKPMN
                     CPGHSLIYKSRLRSYRELPMRLGELGTVYRYERSGVLHGMMRVRGFTQDDAHIYCTPE
                     QVESEILGVLDLCLDFLRTFGYERYDVDLSVWDPSKPQAYAGDAAGWEMGERALRHAL
                     EARSLPFTRREGEAAFYGPKIDIKMYDAIGRAWQGPTVQFDFNLPKRLDITYVGSDSA
                     AAPVIMVHRAIYGSLERFVGGLVEHYAGAFPLWLAPVQAVVLPIADRHREYAAKVRDG
                     LVGAGLRVETDERNEKIGAKIRDAQLLKIPYMLIAGDREAQAGTVAVRSRKEGEVGTK
                     AVEEVRDLLVRMQTERVLGP"
     gene            complement(3123..3197)
                     /locus_tag="VFD06_15865"
     tRNA            complement(3123..3197)
                     /locus_tag="VFD06_15865"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3163..3165),aa:Val,seq:gac)
     gene            3367..4383
                     /gene="aroF"
                     /locus_tag="VFD06_15870"
     CDS             3367..4383
                     /gene="aroF"
                     /locus_tag="VFD06_15870"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZN05062.1"
                     /translation="MLIVMKVDATAEEVRNVEERVRTLGFKPNRIPGSERTAIGVTGN
                     HGPIDPAEFDGLPGVKEAIRVTRPYKLVSREVKAADSVIEVGGKPIGGRRVAIIAGPC
                     AIESEAQTRTIATAVAEAGADFFRGGAFKPRTSPYSFQGLGLEGLKILAAVRKETGLP
                     VITEAVDEESMTMVEDYADAAQIGARNMQNFSLLKRAGRARLPIFLKRGLSATLEELL
                     MAAEYIVSEGNYRVFLCERGVRTFADHSRNTLDLAIIPSVKSVSHLPILVDPSHGTGR
                     RDKVLPLSYAAVAAGADGIMVEVHHEPHRALSDGPQAILPEDFARLVKDVRAVAALFG
                     RDRA"
     gene            4555..>4830
                     /locus_tag="VFD06_15875"
     CDS             4555..>4830
                     /locus_tag="VFD06_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05063.1"
                     /translation="MARTNEIFLIDGDAVMKKTASGARPAAILNGSPAATLVEPRSAA
                     GEGRRSARPAVAATLSMWIWGSGQLLNGDRDLAALLAMWQVQIAALHY"
BASE COUNT          692 a         1638 c         1642 g          858 t
ORIGIN      
        1 gccagctcgg cgagcatctt gcggtcgaga tcgaccccgg ccttcttcag cccttccatg
       61 aagcggctgt aggagaggtc gttgagccgg gccgccgcgt tgatcagcac cgtccagagg
      121 gtgcggaact ggcgcttgcg ctgccgccgg tcgcgatagg cgtagccgag cgacttctcc
      181 accgcctgct tggcgatgcg gtagagcttg ctcttgccga tgtagaagcc cttcgcgagc
      241 ttcagtgtct tcttgcgccg ctggcggcgc ttggttcctc gtttgactcg gggcacggtg
      301 ttctcctagg gacggccgta aggcagcatg gacttcaccc gccgcacgtc ggacttgctg
      361 acgagcgtgt ccatgtccag ctggcgcttc cgcttggtgc tcttggacgt gaggatgtgg
      421 cgcttgtacg ccttgctccg tttcagcttg ccgcgcgccg acatgcgaaa gcgcttcgcg
      481 gcacctctct tggtcttctg cttgggcatg ggctcgggct cctaacgctt gggggccggc
      541 gccgacgccg gcgcagggcc gggcggtggc gctccccccg accatttctt cggggccagg
      601 atcatgtaga gaaacgggcc ctcctggcgc ggctcggact cgacgaggcc gaactcttcg
      661 acctcctgtc gcagccgcct caggatcttg tgcccgatgt cgcggtgcat gttctcgcgg
      721 ccgcggaaga tcaccgtggc cttcaccttc ttccccttgc tcaggaagcg gatcatgttg
      781 ttgcgcttga actcgtagtc gtgttcgtcg gtcttggggc ggaacttcac ctccttgacc
      841 tggatgatcc gctggcgctt cttcgcgtcc tgcgccttct tgttgagctg gtatcggtac
      901 ttgccgtagt cgaggatgcg gcagaccggc gggaccgcgt ccggcgaaac ctccaccagg
      961 tcgagccccg cctccatcgc catccgcagc gcctgctccg gcggcatgat ccccagctgc
     1021 tcgccgttgt cggcgatgac ccgcacctcg cgcgtgcgga tcttctcgtt gattctcagc
     1081 ttcttctcga tggctcctcc ttcagaacct agggcccgag gacccgttcg gtctgcatcc
     1141 gcaccagaag gtcgcgcact tcttcgaccg ccttggtgcc gacctcgcct tcctttcgac
     1201 tccggaccgc gaccgttccc gcctgcgcct cgcgatcccc ggcgatgagc atgtagggga
     1261 tcttcaggag ctgcgcgtcg cggatcttcg caccgatctt ctcgttccgt tcgtccgttt
     1321 cgacgcgcag tccggccccg acgaggccgt cccggacctt cgccgcgtac tcccgatggc
     1381 ggtcggcgat cggcagcacc accgcctgca cgggcgcgag ccacagcggg aacgcgcccg
     1441 cgtagtgctc caccagcccg ccgacgaagc gctccagcga cccgtagatg gcgcggtgca
     1501 ccatgatgac gggggccgcg gccgaatcgg acccgacgta ggtgatgtcg agccgcttcg
     1561 gcaggttgaa gtcgaactgc accgtggggc cctgccacgc ccggccgatg gcgtcgtaca
     1621 tcttgatgtc gatcttcggg ccgtagaagg ccgcctcccc ctcgcgccgg gtgaagggga
     1681 gggagcgcgc ctcgagcgca tgacgcagag cccgctctcc catctcccac cccgccgcgt
     1741 cgccggcgta cgcctgcggc ttcgaggggt cccagaccga caggtcgacg tcgtagcgct
     1801 cgtagccgaa cgtccggagg aagtcgaggc agagatcgag cacgccgagg atctcgctct
     1861 ccacctgctc gggcgtgcag tagatgtggg cgtcgtcctg ggtgaagccg cgcacccgca
     1921 tcatgccgtg caggacgccg ctgcgctcgt agcggtacac cgttcccagc tccccgaggc
     1981 gcatcggcag ctcgcggtac gagcgcaggc gcgacttgta gatgagcgag tggccggggc
     2041 agttcatcgg cttcagcacg taccccatct cgtcgatggg gaacgtgtac atgttgtcct
     2101 tgtagtaatc gaggtggccg gagcgccgcc acagctcgtc cttggcgatg tggggagtgg
     2161 tcaccagctg gtaaccgcgc cggcggtgct cctccttcca gtagtcctcc aggacctgtc
     2221 gcagcacggt gccgtgcgga tgccagaaga tgagccccgg ccccgcggtc tcctcgatgc
     2281 tgaacaggtt gagggccggg cccagccgcc ggtgatcgcg cttcttcgcc tcctcgagca
     2341 tggcgaggtg cttcgcgagc tgctcctcgg tggcgaaggc ggtgccgtag atgcgctgca
     2401 gctgctggtt ctcctccgag cccttccagt aggccccggc gatcgacagc agcttgaagg
     2461 ccccgaggcg cccggtggac ggcacgtgcg ggccggtgca gaagtccatg aacgaatcct
     2521 gccggtagca ggagacgacg tctcccccct tctcgcgcac cagctcgacc ttgaggttgt
     2581 ctccgagccc ctcgaagtac tcgagcgcct ccgccttggg ctgctcgagg cggcggatcg
     2641 ggaagtcgcg ccccttgatc tcccgcatgc gcgcctcgat tcgctccagg tcctccggcg
     2701 tgaagggatc cgggcgctcg aaatcgtaga agaacccctc ctccgtcgcg gggccgatgc
     2761 cgatcttcac ctcggggaac agctccttca cggcgtgggc caggagatgc gaggtggtgt
     2821 gccggtagat ctccagcgcg gcgggggaat ccggggtgat gaagtccacc ttcgcgtcgt
     2881 gccgcagcgg caggtacacg tcccgcgcgg tcccgttctc cgaggcggcg atcatccgct
     2941 tcgcgatctt cgggtcccag gcgtgcgcca gatcctcgaa ggtggtgccg cgcgggaacg
     3001 cgcgaacgct tccgtcgggg aaggtgacct gaatcggatc cacaacctca cctttcattg
     3061 cgggaggcac gcggcgacgt gcgcgccacg ggcggcgggc cccgggaggg ggcggccggg
     3121 ggtggtaggc gcgagcggat ttgaaccgct gacttctacc gtgtcaaggt agcgctctcc
     3181 ccctgagcta cgcgcctgca cctcgtgtcc aggaaagccg cggatcgcgg ccctcggttc
     3241 ttctgcgaag cgcgtagtct aggatcccct ccctcgcatt gtcaactctt ggaaagagtg
     3301 caggttgcgg gtgcggtccc cgcgcgggta tcatcccggg ttccggcctg tgaaggaggc
     3361 gttcccttgc tcatcgtgat gaaggtcgac gcgaccgcgg aggaggtgcg gaacgtcgag
     3421 gagcgcgtcc gcacgctcgg gttcaagccg aaccggatcc cgggatcgga gcgcaccgcg
     3481 atcggcgtga ccggcaacca cgggccgatc gatcccgccg agttcgacgg gcttccgggg
     3541 gtcaaggagg caatccgggt cacccggccg tacaagctgg tcagccggga ggtcaaggcg
     3601 gccgattcgg tcatcgaggt cggggggaag ccgatcggcg gccggcgcgt cgcgatcatc
     3661 gccgggccct gcgccatcga gtcggaagcc cagacccgga cgatcgccac ggccgtggcg
     3721 gaggccgggg ccgacttctt ccgcggcggg gcgttcaagc cgcgcacctc gccctactcc
     3781 ttccaggggc tcggcctcga ggggctgaag atcctcgcgg ccgtccggaa ggagacaggg
     3841 ctgccggtga tcaccgaggc ggtcgacgag gagtcgatga ccatggtgga ggattacgcc
     3901 gacgcggcgc agatcggcgc gcggaacatg cagaacttct ccctgctgaa gcgggccggc
     3961 cgggcgcgcc tgccgatctt cctgaagcgc gggttgtcgg cgactctcga ggagctgctg
     4021 atggcggccg agtacatcgt gtccgagggg aactaccgcg tcttcctgtg cgagcggggc
     4081 gtgcgcacct tcgccgatca ctcccgcaac accctcgacc tcgccatcat cccgagcgtg
     4141 aagtcggtca gccacctgcc gatcctcgtc gacccgagcc acggcaccgg acggcgcgac
     4201 aaggtcctgc cgctgtcgta cgcggcggtc gccgccggcg ccgacgggat catggtcgag
     4261 gtccatcacg agccgcaccg cgccctcagc gacggtccgc aggcgatcct gccggaggat
     4321 ttcgcgcggc tcgtgaagga cgtccgcgcc gtcgccgccc tgttcggccg cgaccgggcc
     4381 tgacccggga ctgacctcga agacactttc ggccacgaat ccgtaccggc cgtgaccgta
     4441 tcgtcacggc ggcgcccctc ccgcctcgtc tttgcgcctg aatttgcgga cgctcgtggt
     4501 acgcaggttg ctcgaccgaa cccggggcag cacatggctc ggagggggaa ggccatggca
     4561 cgtacgaacg agatttttct catcgacggg gacgcggtga tgaagaagac cgcgtccggc
     4621 gcccgcccgg ccgcgatctt gaacgggtcg ccggcggcga ccctcgtgga gccgcgttcc
     4681 gcggcggggg aggggcggcg ctccgcgcgg ccggccgtcg ccgcaacgct gtcgatgtgg
     4741 atctggggga gcgggcagct cctcaacggc gatcgcgatc tggcggcgct cctggcgatg
     4801 tggcaggtgc agatcgccgc gctgcactat
//
LOCUS       DASRIQ010000638         5314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3367029, whole genome shotgun sequence.
ACCESSION   DASRIQ010000638 DASRIQ010000000
VERSION     DASRIQ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3367029"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VFD06_15880"
     CDS             complement(<1..1123)
                     /locus_tag="VFD06_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HZN05064.1"
                     /translation="MSRLPPELAKLQEVIEGHARAYGLDFFPVLFEILDYRMLNQVAS
                     YGGFPTRYPHWRFGMEYEHLSKSYSYGLSKIYEMVINNDPCYAYLLECNATVDQKLVM
                     GHVYAHCDFFKNNLAFSRTNRKMMDDMANHATRIRRAMDRHGVEPVEGFIDLALSLDN
                     LIDPHAPFIVRERSDEPEEPARPVAQRMKSKSYMTDYINPPHLIAAEQKRLEAEAAAQ
                     RHKFPAQDVRDILRFLIDHAPLENWQRDVLSIVRDEAYYFAPQAQTKIMNEGWATYWH
                     SKIMTEKMLTDSELIDYADHHSGTLGGRGRLNPYKLGVELYRDIEDRWNRGRFGKEYD
                     ECDDIRERARWDRGLGLGRDKIFEVRRVCTDVTFIDDYLT"
     gene            complement(1123..2229)
                     /locus_tag="VFD06_15885"
     CDS             complement(1123..2229)
                     /locus_tag="VFD06_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HZN05065.1"
                     /translation="MILRIDQDHNRFRQIVRGRIKQDLKRYISSGELIGRRGKDLISI
                     PLPQIHIPQFIYGKKQQGGVGQGDGPVGTVLGPGEKEGGSHGAGDQPSDHLLEVDVSL
                     DELAEMLGEELELPAIQPKGRKNIVAARDRYVGISRSGPEGLRHFKRTYKETLKRTIR
                     TGAYRPEHPLLMPIRQDKRYRSWKSALLPQANAAILYMMDVSGSMGEEQKEIVRIESF
                     WIDTWLRHHYKGVEIRYIIHDAEAREVDRDTFYHTRESGGTVISAAYRLAARMIEQDY
                     PADEWNVYGFHFSDGDNWSQGDTEECVTLLKTQLLPRVNQFSYGQVESPYGTGQFLVD
                     LKEALGSDERVALSKIENKDGIYQSIREFLGRGR"
     gene            complement(2303..4444)
                     /locus_tag="VFD06_15890"
     CDS             complement(2303..4444)
                     /locus_tag="VFD06_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HZN05066.1"
                     /translation="MPYQKDTAEANIPVGAAETTPSGSPSTAGRDIIQTIARLQDLRG
                     FRDQHWEGSFEDYLEIVRQSPKVTRTAFQRMYDMILAHGTREYHEYKKKIVHYNFFDD
                     KEHGGADAVFGLDVPLMKLVNVFKSAAKRYGTEKRVLLLHGPVGSAKSTIVRRMKRGL
                     EDYSRSPEGAAYSFVWVMDGTAGRRRPDQTPEILACPMHEDPLHLVPDDLRPEVTRML
                     NTGRTDGQTVVIEGDLCPFCRQNYKELTLRYGGDWTKIIGHIRVRRLLLSEKDRVGIG
                     TFQPKDEKNQDSTELTGDINYRKIAEYGSDSDPRAFNFDGEFNVANRGMIEFIEVLKL
                     DVAFLYDLLGASQEHRIKPKKFAQTDIDEVIIGHTNEPEYRKLQHNEFMEALRDRTVK
                     IDIPYITKLAEEIKIYEKDYNAARIKGKHIAPHTLEMAAMWAILTRLEEPKKADLTLM
                     QKLRLYNGKTLPGFTEDNVKELRKEAHREGMDGISPRYIQDKISNALVSDKGEGCINP
                     FMVLNEMESGLRHHSLLTSEDLRKRYRDLLGVIKQEYEDAAKNEVQRAISADEEAIAR
                     LCSNYIDNVKAYTQKERVRNKYTGQNEEPDERLMRSIEEKIDIPDNRKDDFRREIMNY
                     IGALAVEGKSFNYKTNERLHKALELKLFEDQKDSIKLTSLVSSVVDKDTQEKIDVVKS
                     RLKKDYGYCDICATDVLQYVASIFARGDARD"
     gene            4628..>5314
                     /locus_tag="VFD06_15895"
     CDS             4628..>5314
                     /locus_tag="VFD06_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05067.1"
                     /translation="MSRTLHFKLHDLLDRSELRGPRLAEALEQVGREHGFEAFQACLG
                     LVSTRHRSATEARHALSDIEEHRGDLERRLGRDPGFFVAAVDHLRDAEGTGWGRVPAA
                     FPSRACSQVEAPFEDRLEAELRRSERTGRPLVLALLAPRTPLAEPAVTAAAAALERTR
                     RDVDVLARLVPPAFALLLPAIDRPAGERAVQRLIQVAARASGTEWCAGLAERSGTGEE
                     RVEEFAGAALR"
BASE COUNT          740 a         1711 c         1806 g         1057 t
ORIGIN      
        1 cggtcaggta gtcatcgatg aaggtgacgt cggtgcagac gcggcggacc tcgaagatct
       61 tgtcgcgccc gagcccgagg ccgcgatccc agcgcgcgcg ctcccggatg tcgtcgcact
      121 cgtcgtactc cttgccgaag cggccgcggt tccagcggtc ctcgatgtcg cggtacagct
      181 cgaccccgag cttgtagggg ttcagccgcc cgcgtccgcc gagcgtcccg gagtggtgat
      241 cggcgtagtc gatgagctcg ctgtcggtaa gcatcttctc cgtcatgatc ttcgagtgcc
      301 agtaggtggc ccacccctcg ttcatgatct tcgtctgcgc ctgcggagcg aagtaatagg
      361 cctcatcgcg cacgatcgaa aggacgtccc gctgccagtt ctccagcggg gcgtgatcga
      421 tgaggaagcg caggatgtcc cggacgtcct gcgctgggaa cttgtggcgc tgggcggcgg
      481 cctcggcctc gaggcgcttc tgctcggcgg cgatcaggtg cggcgggttg atgtagtcgg
      541 tcatgtacga cttgctcttc atccgctgcg ccaccggccg cgccggctcc tccggctcgt
      601 ccgaccgctc ccgcacgatg aacggggcgt gcggatcgat gaggttgtcg agcgacagcg
      661 ccaggtcgat gaacccctcg accggctcga cgccgtgtcg atccatggcg cgccggatgc
      721 gcgtggcgtg gttcgccatg tcgtccatca tcttgcggtt cgtgcggctg aaggcgaggt
      781 tgttcttgaa gaagtcgcaa tgcgcgtaga cgtgccccat gaccagcttc tggtcgacgg
      841 tcgcgttgca ctccagcaga tacgcgtagc aggggtcgtt gttgatgacc atctcgtaga
      901 tcttcgagag gccgtaggag tacgacttcg acaggtgctc atactccata ccgaagcgcc
      961 agtgggggta ccgggtgggg aagcccccat aggaggccac ctggttgagc atgcggtagt
     1021 cgaggatctc gaacaagacc gggaagaagt ccagcccgta ggctcgcgcg tgcccctcga
     1081 tgacctcctg gagcttcgcc agttccgggg gcagccggct catcagcgtc cccgccccag
     1141 gaattcgcgg atcgactggt agatgccgtc cttgttctcg atctttgaca gggcgacccg
     1201 ctcgtcgctg ccgagcgcct ccttgaggtc gaccaggaac tgcccggtgc cgtaagggct
     1261 ctccacctgt ccgtaggaga actggttgac gcgcggcagc agctgcgtct tcagcagggt
     1321 gacgcattcc tcggtgtccc cctgtgacca gttgtcgccg tcgctgaagt ggaagccgta
     1381 gacgttccac tcgtcggcgg ggtaatcctg ctcgatcatc cgcgcggcca gccggtaggc
     1441 ggcggagatc accgtccctc cggattcacg cgtgtggtag aaggtgtcgc ggtccacctc
     1501 gcgcgcctcg gcgtcgtgga tgatgtagcg gatctccacc cccttgtagt ggtgccgcag
     1561 ccaggtgtcg atccagaagg actcgatccg gacgatctcc ttctgctcct cccccatgga
     1621 accgctcacg tccatcatgt agaggatggc ggcgttcgcc tgcggcagca gcgcgctctt
     1681 ccaggagcgg tagcgcttgt cctgccggat cggcatgagc aggggatgct ccggccggta
     1741 ggcgccggtt cggatggtgc gcttcagcgt ctccttgtag gtgcgcttga agtgccgcag
     1801 gccctccggc ccggagcggg agatgccgac gtagcgatcg cgcgccgcga cgatgttctt
     1861 gcgccccttc ggctggatcg ccggcagctc gagctcctcg ccgagcatct cggccagctc
     1921 gtcgagcgac acgtccacct cgaggaggtg gtcggacggc tggtcgccgg cgccgtggct
     1981 gccgccctcc ttctccccgg ggcccaggac ggtcccgacc ggaccgtccc cctggccgac
     2041 gcccccctgc tgcttcttgc cgtagatgaa ctgcgggatg tggatctgcg gcaggggaat
     2101 ggagatgagg tccttgccgc gccggccgat cagctcgccg ctgctgatgt agcgcttcag
     2161 gtcctgcttg atccgtccgc gcacgatctg gcggaagcga ttgtggtcct ggtcgatgcg
     2221 gagaatcatg ccggcgtccc gatcggctcc gccccggccc gggagccttc gcggccggtg
     2281 cggtgcgtca agggtcctcc cctcagtcgc gggcgtcgcc gcgcgcgaag atgctggcga
     2341 cgtactgcag gacgtcggtg gcgcagatgt cgcagtagcc gtagtccttc ttgaggcggc
     2401 tcttcacgac gtcgatcttc tcctgcgtgt ccttgtcgac gaccgaggag acgagcgacg
     2461 tcagcttgat ggagtccttc tggtcctcga acagcttcag ctcgagcgcc ttgtgcagcc
     2521 gctcgttggt cttgtagttg aagctcttcc cttcgacggc cagcgccccg atgtagttca
     2581 tgatctcgcg ccggaagtcg tccttgcggt tgtccgggat gtcgatcttc tcctcgatcg
     2641 accgcatcag gcgctcgtcc ggttcttcgt tctggccggt gtacttgttg cggacccgct
     2701 ccttctgggt gtacgccttg acgttgtcga tgtagttgct gcacagccgg gcgatcgcct
     2761 cctcgtcggc ggagatggcg cgctgcacct cgttcttggc ggcgtcttcg tactcctgct
     2821 tgatcacgcc gagcaggtcg cggtagcgct tgcgcagatc ctcggaggtg aggagcgagt
     2881 ggtgccgcag ccccgactcc atctcgttga ggaccatgaa cgggttgatg cagccctctc
     2941 ccttgtccga caccagggca ttgctgatct tgtcctggat gtagcggggc gagatgccgt
     3001 ccattccctc gcggtgggcc tccttgcgca gctccttgac gttgtcctcg gtgaagccgg
     3061 ggagcgtctt gccgttgtag aggcgcagct tctgcatcag ggtgaggtcg gccttcttcg
     3121 gctcctcgag ccgggtcagg atcgcccaca tcgcggccat ctcgagggtg tgcggcgcga
     3181 tgtgcttccc tttgatgcgg gcggcgttgt agtccttctc gtagatcttg atctcttccg
     3241 ccagcttggt gatgtagggg atgtcgatct tcacggtccg gtcccgcagc gcctccatga
     3301 actcgttgtg ctggagcttg cggtactccg gctcgttggt gtgaccgatg atcacctcgt
     3361 cgatgtcggt ctgcgcgaac ttcttcggct tgatccggtg ctcctgcgag gcgcccagaa
     3421 ggtcatagag gaacgccacg tccagcttca gcacctcgat gaactcgatc atgccgcggt
     3481 tggcgacgtt gaactcgccg tcgaagttga aggcgcgcgg atcggaatcg gagccgtatt
     3541 cggcgatctt gcggtagttg atgtcgccgg tcagctcggt cgagtcctgg ttcttctcgt
     3601 ccttcggctg gaaggtgccg atgccgacgc ggtccttctc cgagaggagc agccgccgca
     3661 ccctgatgtg gccgatgatc ttcgtccagt cgccgccgta gcggagcgtc agctccttgt
     3721 agttctggcg gcagaagggg cacaggtccc cctcgatcac gaccgtctgg ccgtcggtgc
     3781 ggccggtgtt gagcatgcgg gtcacttccg gccgaagatc gtccggaacg aggtgcagcg
     3841 ggtcttcgtg catcgggcag gcgaggatct ccggcgtctg gtccgggcgc cggcggccgg
     3901 ccgtgccgtc catcacccac acgaacgagt aggccgcccc ctcgggcgag cgggagtagt
     3961 cctcgaggcc gcgcttcatg cgccgcacga tcgtcgattt ggctgatccg accgggccgt
     4021 gcagcagcag cacgcgcttc tcggtgccat agcgctttgc cgccgacttg aagacgttga
     4081 ccagcttcat cagcggcacg tcgaggccga agacggcgtc ggcgccgccg tgctccttgt
     4141 cgtcgaagaa gttgtagtgg acgattttct tcttgtactc gtgatactcg cgcgtcccgt
     4201 gcgcgaggat catgtcgtac atgcgctgaa aggcagtccg cgtcaccttc gggctctggc
     4261 gcacgatctc gagataatcc tcgaacgatc cttcccagtg ctggtcgcgg aagccgcgca
     4321 gatcctggag gcgcgcgatc gtctggatga tgtcgcggcc ggcggtggag ggggaaccgg
     4381 agggcgtggt ctctgcggcg ccgaccggga tgttcgcttc agccgtatct ttctgatagg
     4441 gcatcgtttc ctcggcgcgt tcaaacgcgt cagtcaatat agggtcgagt caaggatttg
     4501 tcaaaaccgg atcacgattt tctccggtgg cggagccgcg ttcgcacgcg cgcccgttga
     4561 cagcccgaag cgtcggccgc taatcttccg gcgcgccccg cgggaagcgg cctgcggggg
     4621 gcgggcgatg agccggaccc tccacttcaa gctgcacgat ctcctggacc gttccgagct
     4681 gcgcgggccg cggctggcgg aggccctgga gcaggtcgga cgcgagcacg gattcgaggc
     4741 gttccaggcc tgcctcggcc tcgtctcgac ccggcaccgc tcggcgaccg aggcccggca
     4801 cgccctttcg gacatcgaag agcatcgtgg cgatctcgag cgccgcctcg ggcgcgatcc
     4861 cggcttcttc gtcgcggcgg tggaccacct gcgcgacgcg gaagggacgg gctggggccg
     4921 cgtgccggcc gcgttcccat cccgcgcctg ctctcaggtc gaggcgccgt tcgaagaccg
     4981 cctcgaggcg gagctgcgcc gatcggagag gaccggccgg ccgctcgtgc tggcgctcct
     5041 cgctccgcgg acaccgctcg cggaaccggc cgtgacggcc gcggcggcgg cgctcgaacg
     5101 gacgcggcgg gacgtcgacg tgctggcgcg gctggttcct cccgccttcg ccctcctgct
     5161 gccggcgatc gaccgcccgg cgggggagcg ggcggtgcag cgcctgatcc aggtggcggc
     5221 ccgcgcctct ggaaccgaat ggtgcgccgg cctggcggaa cgaagcggca cgggggagga
     5281 gcgagtcgag gagttcgccg gcgcggccct gcgc
//
LOCUS       DASRIQ010000639         5019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2076648, whole genome shotgun sequence.
ACCESSION   DASRIQ010000639 DASRIQ010000000
VERSION     DASRIQ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076648"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..477
                     /locus_tag="VFD06_15900"
     CDS             70..477
                     /locus_tag="VFD06_15900"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HZN05068.1"
                     /translation="MYTVTEILDFCYGHRLLRYQGKCAHLHGHNGRVEIVVAAKTLDR
                     QSMVTDFSEIQRVVRGFIDEHLDHKLLLAKDDPLVDVLKKQDEPIFVMDSDPTAEAIA
                     RLICDKARAAGLDVAEVRLWETPASVASYVPSR"
     gene            complement(1153..1974)
                     /locus_tag="VFD06_15905"
     CDS             complement(1153..1974)
                     /locus_tag="VFD06_15905"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HZN05069.1"
                     /translation="MPRAPGTKARRRAAGPPACVLVSGGVDSIVLLRETLKRHKSVQP
                     LYVRAGMAWEPVERRALARLLRLLRGSRLRPLRTIDVPMGDLLARHWSATGRDGPGYD
                     DGDASVFIPGRNIALFSKAATFCTLERIPVLVSGILSANPFPDGSPRFLRAMERGLAL
                     GLGARFRIATPFRRRTKDEVIRLGRDLPLHLTFSCISPRRGLHCGDCCKCKERIDAFV
                     AAGVADRTRYARPAPASSGGAGSVPRGRPARSASAGGATARIMTRQAAKSATSAR"
     gene            complement(1994..2311)
                     /locus_tag="VFD06_15910"
     CDS             complement(1994..2311)
                     /locus_tag="VFD06_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZN05070.1"
                     /translation="MSYVIIDKCVGERYATCVAVCPVDCIHPGEYKGEQFMIIDPEEC
                     IDCGACLPECPIEAIVETEEESPDWAVINKDLTPSFKQNAPVTPRPANDPPRKPENKL
                     RSS"
     gene            2467..>5019
                     /locus_tag="VFD06_15915"
     CDS             2467..>5019
                     /locus_tag="VFD06_15915"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZN05071.1"
                     /translation="MTVRFARVPLAAAGLLLAAALPLSAGFAEFPSASVPTPPSGRET
                     VLKQIKVPHNYYYREMYLPQVTSGPTGADWSPDGRRIVLSMRGSLFIHDLEKRETRQV
                     TDGPGYDYQPDWSPDGRFIAYASYRDDAVDLFLHEVATGASRPLVQNGAVNVEPRFSP
                     DGERIAYVSTATEGRFHVFTVAVKDPVAGKPAAPEQVTEDRDGGLPRYYYSRFDHYLS
                     PAWSPDGNELLVVSNRGQIWGTGGFWRLRARGGKMRPVHDEETTWKARPDWAPDGKRI
                     VYPSYAGRQWHQLWLMTADGGDPLQLTYGEYDMTAPRFSADGRRIVCISNEPRPARGG
                     GRRDADPGTHLVIVDLFGGARTVDLGTIVPFGPRGTLRLSILDGGGSPMAARVSVMGE
                     DGRFHAPDDAWRHADEAFVRGESRFEYGYFHARGSATLSLAPGRYRVEVSRGPEFKVH
                     RRDVTVARGSTTAHRASLARLKDMPASGWRSADLHVHMNYGGAYRCDPRRLAFQAEAE
                     GLNLIENLIVNKEQRVPDISYFSPRPDPASTSDVLIVHGQEFHTSYWGHTALIGLDDH
                     LILPDYVSYTNTAVASPYPHNAAVFDLAHAQKALTGYVHPFDTRPDPFKMDEPLAYEM
                     PADAVLGKMDYFEVMGFSDHLITSEIWYRLLNIGLRIPAGAGTDAMANYASLRGPVGL
                     VRVFVRTGPQFNHGTLLAGLKAGRTFVTNAPLLDLGVRAKGAGAVSSAGDGTFKGPGS
                     EIRLAAGKHNLEARVDVRSPVALDHVELVANGRTVGTVPLAADRMSARGSVAVPFEAS
                     GWIVLRAWADKPAWPILDLYPFASTSPVYVRVGDEEPRSPEDAAYFVKWLDRV"
BASE COUNT          725 a         1767 c         1746 g          781 t
ORIGIN      
        1 accgggaggg atgcggatcg aggcaccaca ctcttcgcac cacgttgggt gtgatagctt
       61 tcgaggctca tgtacacggt caccgagatc ctggatttct gctacggcca ccgcctcctc
      121 cgctaccagg ggaaatgcgc ccacctccac ggccacaacg ggcgggtgga gatcgtcgtc
      181 gcggccaaga cgctcgaccg gcagtcgatg gtcaccgact tctccgagat ccagcgggtg
      241 gtgcgcgggt tcatcgacga gcacctcgat cacaagctgc tgctggcgaa ggacgaccct
      301 ctggtggacg tcctgaagaa gcaggacgaa ccgatcttcg tcatggactc cgacccgacc
      361 gccgaggcga tcgcgcggct catctgcgac aaggcgcgcg ccgccgggct cgacgtggcc
      421 gaggtgcgct tgtgggagac gcccgcttcg gtggcgtcgt acgtgccgtc gcgctgacgt
      481 cgatcttttc cacttgagcc tgctgaagga atcgttcgcg gggatcaacg cccgcttcca
      541 cctcgtcggg atcagcaccc tgctcagcct gctcctgccg atggcggttt cggcggcggg
      601 gaagaaggaa gagtggaccc cggcgctctt cgccgggttc ttcgtcgcct ggggcgtcgc
      661 ctttgccatc caggggctcg tctaccacgc ggcgatcgac gatgcgcgga gcatcaagcc
      721 gtccgcggtg cacctcgggc tgcagctctt cccgaccctc ctgtggctcc aggtgaagct
      781 cttctttctg tgcgccgtgc cgctcctggc cgcggcgctg gggtggcagg ccgcccagtc
      841 ccaaggccag gtgacggaag ccgcggtgcg gcaggccgat tactggatca atccggtggc
      901 ggagaccgcc atcctgctcc tgacgacggc ggcgacgccg ctcgccatct ggctgaagga
      961 gcacggccgg cgcggacggc cgatcgtgga cggggtgcgt ttcttctcgc gccggccgcg
     1021 ggagaccacc cgcatcctcg cgcttctcgt gccggcggcg attctggggg ggacgatcga
     1081 ctacctgagg ggacccacgc tcgaggaccc ggttccccgc ctgcctgaag ccttcgcgat
     1141 gttcctgacg tcctacctgg cgctggtggc gctcttcgcc gcctgtcgcg tcatgatccg
     1201 ggccgtggcg cctccggccg atgccgatcg ggccgggcgc ccgcgcggca ccgaacccgc
     1261 tcctcccgag gatgcggggg ccgggcgcgc gtagcgggtc cgatccgcga cgccggcggc
     1321 gacgaaggcg tcgattcgct ccttgcactt gcagcagtcg ccgcagtgga gacctcggcg
     1381 cgggctgatg caggagaagg tcaggtggag cggcaggtcg cgcccgagcc ggatgacttc
     1441 gtccttggtg cgccggcgga agggggtggc gatccggaag cgggccccca gcccgagcgc
     1501 cagcccccgc tccatcgccc gcaggaagcg cggcgatccg tccgggaacg ggttggcgct
     1561 caggatgccc gagacgagca cggggatccg ctcgagggtg cagaaggtgg ccgccttcga
     1621 gaacagcgcg atgttccgcc ccgggatgaa gaccgaggcg tcgccgtcgt cgtagccggg
     1681 cccgtcacgt cccgtggcgc tccagtggcg ggcgagcagg tcccccatcg gcacgtcgat
     1741 ggttcgaagc ggccgcaggc gcgaacccct gagcaggcgg agcagacggg cgagggcgcg
     1801 gcgctccacc ggctcccacg ccatgccggc gcgcacgtac agcggctgca cgctcttgtg
     1861 acgcttcagg gtttcgcgca gcaggacgat cgaatcgacg ccgccgctga ccaggacgca
     1921 cgccggcggt ccggcagcac gtcgccgtgc cttggtgccg ggcgcgcgcg gcatctttcg
     1981 ctcgggagga cgctcaggag gagcgcagct tgttttcggg cttcctcggg ggatcgttgg
     2041 cggggcgcgg ggtgaccggg gcgttctgct tgaacgacgg ggtcaggtcc ttgttgatga
     2101 cggcccagtc gggggactcc tcctcggtct cgacgatcgc ctcgatcggg cactccggca
     2161 ggcaggcgcc gcagtcgata cactcctcgg ggtcgatgat catgaactgc tcgcccttgt
     2221 actccccggg atggatgcag tcgacgggac agaccgcgac gcacgtggcg tagcgctccc
     2281 ccacacactt gtcgatgatc acgtaggaca cgtcccagcc tcctgtcaga tcgtcgtttg
     2341 ctcggtacga gcccggcgat gttagcacag gggaacgggg gcgagagcgg ggtctgcggc
     2401 gggcggacgg ggtccccggg aggccggtcg gtctagaatc cgcccttcga aacagtgggg
     2461 ggtcgcatga ccgtccgatt cgcccgggtg ccgctcgccg ccgccggcct ccttctggcc
     2521 gccgccctcc cgctctcggc cggcttcgcc gagttcccgt ccgcctcggt cccgacaccg
     2581 ccgtcggggc gggagacggt cctcaagcag atcaaggttc cccacaacta ctactaccgg
     2641 gagatgtacc tgcctcaggt gacgagcggc cccacggggg ccgactggtc cccggacggc
     2701 cggcggatcg tcctgtcgat gcgcggctct ctcttcatcc acgacctcga gaagcgtgag
     2761 acgcggcagg tgaccgacgg gccgggctac gactaccagc cggactggtc gccggacggc
     2821 cgcttcatcg cctatgcctc gtaccgcgac gacgccgtgg acctgttcct ccacgaagtc
     2881 gcgaccgggg cgagccgacc gctggtgcag aacggcgccg tcaacgtcga gccgcgcttc
     2941 tctcccgacg gcgagcggat cgcctacgtc tcgaccgcga ccgaggggcg gttccacgtc
     3001 ttcaccgtcg cagtgaagga cccggtggcg gggaagccgg ccgctccgga gcaggtgacc
     3061 gaggaccgcg acggcgggct gccgcgctac tactacagcc gcttcgatca ctatctctcc
     3121 cccgcctggt cgccggacgg caacgagctc ctggtcgtgt cgaaccgcgg ccagatctgg
     3181 ggaaccggtg gcttctggcg gctgcgcgcc cggggcggga agatgcgccc ggtgcacgac
     3241 gaggagacca cctggaaagc acgtcccgac tgggcgccgg acggaaagcg gatcgtctac
     3301 ccctcctacg ccggccggca gtggcaccag ctgtggctca tgaccgcgga cggtggcgac
     3361 ccgctgcaac tcacctacgg cgagtacgac atgacggcgc cgcgcttctc ggccgacggc
     3421 cggcgcatcg tctgcatctc gaacgagccg cggccggcac gcggcggtgg ccgccgcgac
     3481 gccgatcccg gcacgcacct cgtcatcgtc gatctcttcg gcggggcgcg gacggtcgat
     3541 ctcgggacga tcgtcccgtt cgggccgcgc gggacgctgc gcctgtcgat tctcgacggc
     3601 ggcggatctc cgatggcggc gcgcgtctcg gtcatgggcg aggacgggcg cttccacgct
     3661 ccggacgacg cctggcggca cgcggacgag gccttcgtcc gcggcgagag ccggttcgag
     3721 tacggctact tccacgcccg cggctcggcg accctgtcgc tcgctccggg gcgctaccgg
     3781 gtcgaggtga gccgcggtcc cgagttcaag gtgcaccgtc gtgacgtgac cgtggcgcgc
     3841 ggctcgacca cggcgcaccg ggcctccctc gcgcggctca aggacatgcc cgcgtcgggc
     3901 tggaggagcg ccgacctgca cgtccacatg aactacggcg gcgcctaccg gtgcgacccg
     3961 cgccggctgg cgttccaggc tgaggccgaa gggctgaacc tgatcgagaa cctcatcgtc
     4021 aacaaggagc agcgcgtccc cgacatctcg tacttctcgc cccgccccga tccggcgtcc
     4081 acctccgacg tcctgatcgt ccacgggcag gagttccaca cctcgtactg ggggcacacg
     4141 gccctgatcg ggctcgacga ccacctgatt ctgcccgact acgtgtcgta cacgaacacg
     4201 gcggtcgcga gcccgtaccc gcacaacgcc gcggtcttcg acctcgcgca cgcgcagaag
     4261 gcgctcaccg gctacgtcca cccgttcgac acgcgccccg acccgttcaa gatggacgag
     4321 ccgctggcct acgagatgcc ggccgacgcg gtgctcggca aaatggacta cttcgaggtg
     4381 atggggttca gcgatcacct gatcacgtcc gagatctggt accgcctgct caatatcgga
     4441 ctgagaattc ccgccggcgc cggcaccgac gcgatggcga actacgcgtc gctgcgcggc
     4501 cccgtcggtc tcgtgcgcgt cttcgtccgg accgggccgc agttcaacca cgggacgctc
     4561 ctcgcggggc tcaaggcggg gcgcaccttc gtgaccaacg caccgcttct cgatctcggc
     4621 gtgcgggcga aaggcgccgg cgctgtgtcg agcgccggtg acggaacctt caaggggccg
     4681 ggatcggaga tccgcctcgc cgcggggaaa cacaacctcg aggcgcgcgt ggacgtgcgc
     4741 tcccccgtgg cgctcgatca tgtcgagctc gtcgccaacg gcaggaccgt cggcaccgtg
     4801 ccgctcgccg ccgaccggat gtcggcgcgc ggctccgtcg ccgttccttt cgaggcgagc
     4861 ggctggatcg tgctgcgcgc ctgggccgac aagccggcct ggccgatcct cgacctctac
     4921 cccttcgcct cgacgagccc ggtgtacgtg agggttggcg acgaggagcc gcgctcgccg
     4981 gaggacgccg cctacttcgt gaagtggttg gaccgggtc
//
LOCUS       DASRIQ010000640         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2077076, whole genome shotgun sequence.
ACCESSION   DASRIQ010000640 DASRIQ010000000
VERSION     DASRIQ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077076"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..834)
                     /gene="tpiA"
                     /locus_tag="VFD06_15920"
     CDS             complement(76..834)
                     /gene="tpiA"
                     /locus_tag="VFD06_15920"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZN05072.1"
                     /translation="MTSPRRALVVGNWKMQMPISTAAETARAIARTLPALPDRDVVVA
                     PPFPALLPVAEALRGSPVQLAAQDLFWKDDGPFTGEVSGPMLVEAGVRAVIVGHSERR
                     QHLGETDAMVGLKTWAALRAGLVPIVCVGEREEDRDSARHERIVRDMLLRSLESLPKE
                     AADTLVVGYEPLWAIGTGCAASAADAAEMHACLRKALETHFGPAATRIRIVYGGSVTE
                     QNIDELMAQPEVDGALVGGASLRPESFVRIAAFR"
     gene            complement(831..2048)
                     /locus_tag="VFD06_15925"
     CDS             complement(831..2048)
                     /locus_tag="VFD06_15925"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZN05073.1"
                     /translation="MSHTLSVREIPIAHRRVFVRVDFNVPLEAGRIADDTRLRAALPT
                     LRLLLDGGASIVLASHLGRPKGKPKPDASLRPVAARLQEMLGRPVILAPDCVGEEVEQ
                     MARALAPGGLLLLENLRFHAEEERNDDGFARRLAALGEVYVNDAFGAAHRAHASVAAI
                     AAHLKPAAAGLLMDREIGMLSRLRDRPEKPYVALLGGAKVSDKIDLIEHLLERVDAIL
                     VGGAMAYTFLKAAGVSVGGSRVEADRVDAARDTAARAEERGVRLLLPIDHVVADGPEP
                     GRPSEATPGAAIPDGRVGLDVGPRTRDVFAQELAHARTVFWNGPLGLFEVPPYDQGTL
                     AAARAIAAAEGIFSVVGGGDSVAAITRLGLADRFAHVSTGGGASLEFLSGLELPGVAA
                     LDTAPGDAPVAVR"
     gene            complement(2048..>2625)
                     /locus_tag="VFD06_15930"
     CDS             complement(2048..>2625)
                     /locus_tag="VFD06_15930"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZN05074.1"
                     /translation="VVSNASCTTNALAVVLDVAIRRFGIERAAMTTIHCVTNNQPLVD
                     APHADPRRARGALHSMIPTTTSAAEAVVAVLPAMRGRLHALAVRVPTAGVSLVDLTIA
                     LSRPATPESARATYREEAEGRLAGILGFCDEPLVSADYAGDTHSAVVDGPLLAVEGDR
                     WLKILAWYDNERGYVERLADLVRRMAPKGAR"
BASE COUNT          360 a          964 c          979 g          322 t
ORIGIN      
        1 cgggaggccc gggacgaaga ggggaggcct tccttttccc cccggccggg acctcccccg
       61 gggcccggcc ggggcctagc ggaatgccgc gatgcggacg aaggactcag gccgcagcga
      121 ggcgcccccg accagcgccc cgtccacctc gggttgcgcc atcagctcgt cgatgttctg
      181 ctcggtgacg ctgccgccgt agacgatgcg gatgcgggtg gcggcggggc cgaagtgcgt
      241 ctcgagtgcc ttgcgcaggc aggcatgcat ctcggcggcg tcggcggcgc tggcggcgca
      301 gccggtgccg atcgcccaca acggctcgta gccgacgacg agggtgtccg cggcctcctt
      361 cggcaggctc tcgagcgagc gcagcagcat gtcacggacg atccgctcgt gccgcgccga
      421 gtcccggtcc tcctcccgct cgccgacgca gacgatcggg acgagccccg cccggagcgc
      481 cgcccaggtc ttgaggccga ccatcgcgtc ggtctcgccc aggtgctggc ggcgctcgga
      541 atgcccgacg atgacggcgc ggacgccggc ttccacgagc atcggcccgg agacctcccc
      601 cgtgaagggg ccgtcgtcct tccagaagag atcttgcgcc gcgagttgca ccggggagcc
      661 gcgcagcgcc tccgccaccg ggaggagcgc cgggaacggc ggcgccacca ccacgtcccg
      721 gtccggaagc gccgggaggg tccgggcgat ggcgcgggcg gtctcggccg cggtggagat
      781 cggcatctgc atcttccagt tgccgacgac gagcgcacga cggggggagg tcatcgcacc
      841 gcgaccgggg cgtcgccggg ggcggtgtcg agcgccgcga cccccggaag ctcgagcccg
      901 gacaggaact ccagcgaggc gccgcctccg gtggagacgt gggcgaaccg gtcggcgagg
      961 ccgagccggg tgatcgcggc caccgagtcc ccgccgccga cgacgctgaa gatcccctcc
     1021 gccgccgcga tcgcccgcgc cgcagcgagc gtcccctgat cgtagggggg aacctcgaag
     1081 agtccgagcg ggccgttcca gaacacggtg cgcgcgtggg cgagctcctg ggcgaagacg
     1141 tcgcgcgtcc tcggccccac gtcgagcccg accctcccgt ccgggatcgc cgcgccgggg
     1201 gtcgcctcgc tcggccgccc cggctccggg ccgtcggcga cgacgtgatc gatcggaagc
     1261 aggagccgca cgccccgctc ttccgcccgc gccgcggtgt cgcgcgccgc gtcgacgcgg
     1321 tccgcttcga cccgcgaccc gccgacagaa acaccggccg ccttcaggaa ggtgtaggcc
     1381 atggccccgc cgacgaggat cgcgtcgacg cgctcgagca ggtgctcgat gagatcgatc
     1441 ttgtcggaca ccttcgcgcc gccgaggagc gccacgtacg gcttctccgg ccgatcgcgc
     1501 aggcgcgaca gcatgccgat ctcccggtcc atcaggagcc cggcggccgc cggcttgagg
     1561 tgcgcggcga tcgccgcgac cgaggcgtgc gcccggtgtg ccgccccgaa ggcgtcgttg
     1621 acgtagacct ccccgagggc ggccaggcgg cgggcgaacc cgtcgtcgtt ccgctcctcc
     1681 tccgcgtgga agcgaaggtt ctcgaggagg agcaggccgc ccggcgcgag cgcgcgggcc
     1741 atctgttcga cctcctcgcc gacgcaatcc ggcgcgagga tcaccggccg gcccagcatc
     1801 tcctggaggc gcgcggcgac cggccgcagg ctggcgtccg gcttcggctt ccccttcggc
     1861 cgcccgaggt gcgacgcgag gacgatcgac gccccgccgt cgaggagcag gcggagcgtc
     1921 ggcagcgcgg cgcggaggcg ggtgtcgtcg gcgatccgtc ccgcctcgag cgggacgttg
     1981 aaatcgacgc gcacgaagac gcgccggtgg gcgatgggga tctcgcgcac ggagagcgtg
     2041 tgggacatca acgggccccc tttggcgcca tgcggcgcac gaggtcggcg aggcgctcga
     2101 cgtagccgcg ttcgttgtcg taccaggcca gaatcttcag ccagcgatcc ccctccaccg
     2161 cgagcagcgg accatcgacg acggccgagt gcgtgtcgcc ggcgtagtcg gccgacacca
     2221 gcggttcgtc gcagaagccc aggatcccgg cgagacgccc ctcggcctcc tcacggtacg
     2281 tcgcccgggc cgactccggc gtcgcgggcc gggacagggc gatggtcaga tcgacgagcg
     2341 acacgccggc cgtcggcacc cgcaccgcga gggcgtgcag ccggcccctc atcgccggca
     2401 gcacggcgac caccgcctcg gccgccgatg tggtggtcgg gatcatcgaa tgcagcgcgc
     2461 cgcgggcgcg gcgcggatcg gcgtgcggcg cgtccacgag cggctggttg ttggtgacgc
     2521 agtggatggt ggtcatggcc gcccgctcga tgccgaagcg gcggatcgcc acgtcgagga
     2581 cgaccgccag ggcgttcgtg gtgcacgagg cgttcgacac cacga
//
LOCUS       DASRIQ010000641         5179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1035864, whole genome shotgun sequence.
ACCESSION   DASRIQ010000641 DASRIQ010000000
VERSION     DASRIQ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035864"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VFD06_15935"
     CDS             <1..1304
                     /locus_tag="VFD06_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05075.1"
                     /translation="FVGLMGTADIGPINGEPAGLSAQAMTEFRRFALDIVFPPNPYRN
                     VDDTVPNASVAIPGLPNPGNPEIGRQRFLGLTGGFTDGASFCVSCHQLPFGAAGGKIG
                     GLEPGDPADARAALFNGDADLSPHSDVKIPHMRNMYEKFGPRYGTVASPLDQKSGFGL
                     THDGATPDMNSFLSAGVFTINADDVRNISAFSMMFPTGTRPSVGRNLTLPQGVPPTGT
                     TAQETLLSQLIALGNRGDPNRQCELYAAGRSSGAGARERTWYLNGGAPGGFWSTDVTS
                     ELQITTAQLRTNAGGPLTFTCATVGSGIRLGADRDEDGVPNGSDFCPGEPAVSQAPGL
                     VNGVLVDASSQVTWDPLASPDGAAIQYDVAGGNLSAVPGSGFAGAACLAGGLPAIGWT
                     DLRTAPPVGDGYYYLVRGVKSCGAGSFGPGLAGPDTLRCSP"
     gene            complement(1254..2402)
                     /locus_tag="VFD06_15940"
     CDS             complement(1254..2402)
                     /locus_tag="VFD06_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN05076.1"
                     /translation="MSARAREAAANALLVVGSLATLFLLLEVAVRLFLPVADIPWHEG
                     DPVVGYRLAPNQSGTWFGEGIHATYRINGSGFNNEHEYTVERTPGHARVAVLGDSYVE
                     ALYIDRGRRFFDVLEAGLRGAGEPADVFTYAVSGYGTGQELLLLQNEVVRDRPDLVIL
                     LWVSNDLSDTACAVSRGAEGPCFYFDDAGHLAHRPPRPHAPNRKVRLVLNSALARYVA
                     INLNAVGLWRAIWTDGGPRGEPWTMTYARTPPPDWVEAWRVFDGCILEMARFCRDEGI
                     PFLLVHQPIVSRHFRALLADVPGGGDVSLPARRFADLASRHGFRFFDLTPAFLVEGRI
                     DPKRWINVGVGHWNPAAHEAAGTALVAPVLDLLGGAAQGVGAGEPGAE"
     gene            complement(2399..5020)
                     /locus_tag="VFD06_15945"
     CDS             complement(2399..5020)
                     /locus_tag="VFD06_15945"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZN05077.1"
                     /translation="MPRPRAFSPVLLPVVPLLLGAAFLLGAAPAAPPEADPLAGATSF
                     TLDNGLTCVLRPDAAHPVVAVQMLYRVGARNETIGITGIAHYLEHMLFRGTERFGLTD
                     VTGVIERAGGEWHGYTTLDCTTYFQAAPKETLATLLRLEAERMVSGRMAADEVEPERG
                     AVFQEYRGYQLDARSDLFDATTALLFEQHPYRNNTMGWESDLAGITHADLVAFYRRYY
                     GSGNAVLAIAGDIDAAAARRAIEEIFGPIPKGGADTTIRTVEPPLEGPRRLTLLRAGA
                     TPALQISFLAPPPSRPREFAALLLLEALIGNAAGLSFYRHSGDLGTGGGAPAGSPLGR
                     LVETGLLEEAGASLVPTLYPYHFTLYASGFEPAKASEAEAALFDLLAEAADGADAADA
                     ASARRRIAAADLLETDSLVEKAHEMAYWTALGGLELRSQVRAAVDAVTAEDVRAAAAA
                     LVPSRAAIGLVLPAARSGPIGGIAVPPTAERPKRPAPARPIGAPGIATKRVARAQIGT
                     LGLGSGARAIVDARPGAPTFVLHLAVAAPAGTALANEDDLARAFVPLGVTLEVTLPGR
                     GPFAARDTVLLEMAGPAGAFDDAIGMLQSRGLKLSKPKPGPSTVDPESLSRPMRRAMA
                     YLDRAAGPAATSSSPGASGRPSGPFFALVGPFDPGAVKDRLASFAAPLGGAPPTRRAS
                     AAGLQPGRDISAIRDIPQGALVLAIPGDADAMAQEAVAWILQHNYSGRLGTRAIAQMG
                     LVYDMDSESVRRGAPLVWFGMGADPDALGRLEEALVAELARARRDIGDEEIAAFKSFT
                     AGRLAVRRADPEQAARLWLAALLRGEDDRAVAHEADRAARLSRSDVTAAAERMLAPER
                     LRVVVVGRAPRETAPGR"
BASE COUNT          728 a         1833 c         1956 g          662 t
ORIGIN      
        1 cgttcgtggg gctgatgggg accgccgaca tcgggccgat caacggcgag ccggccggcc
       61 tttccgcgca ggccatgacg gagttccgcc gcttcgcgct ggacatcgtc ttcccgccga
      121 atccgtaccg caacgtcgac gacaccgtgc cgaacgcgtc ggtggcgatc ccggggctgc
      181 cgaacccggg gaacccggag atcggccgcc agcggttcct ggggctgacc ggggggttca
      241 ccgacggggc gtcgttctgc gtctcctgcc accagctgcc gttcggcgcc gccggcggaa
      301 agatcggcgg cctggagccg ggcgacccgg cggacgcgcg ggcggcgttg ttcaacggcg
      361 acgccgatct gtcgccccac agcgacgtga agatcccgca catgcgcaac atgtacgaga
      421 agttcggacc gcgctacggc accgtggcga gccctctgga tcagaagagc ggcttcgggc
      481 tgacgcacga cggcgccacc ccggacatga acagcttcct gtcggccggc gtcttcacca
      541 tcaacgcgga cgacgtgcgg aacatctccg ccttctcgat gatgttcccg accgggaccc
      601 ggccgtcggt cgggaggaac ctcacgctgc cgcagggagt cccccccacc ggcacgacgg
      661 cgcaggagac gctcctgtcg cagctgatcg ccctgggcaa tcgcggcgac ccgaaccgcc
      721 agtgcgagct gtacgccgcc ggccgatcgt ccggggccgg cgcgcgcgag cgcacctggt
      781 acctcaacgg cggcgcgccg ggaggattct ggtccaccga cgtcaccagc gagctgcaaa
      841 tcaccacggc gcagctccgc acgaacgccg gggggccgct gaccttcacc tgcgccaccg
      901 tcggctcggg gatccggctc ggcgccgacc gcgacgagga cggcgtcccg aacggcagcg
      961 atttctgtcc gggcgagccg gccgtttcgc aggcgccggg gctcgtcaac ggcgtcctgg
     1021 tggacgcgtc ctcgcaggtg acgtgggatc cgctggcgtc gccggacggc gcggcgatcc
     1081 agtacgacgt ggctggagga aacctttcgg ccgtgcccgg gagcggcttc gcgggagccg
     1141 cgtgcctcgc ggggggcctg ccggcgatcg ggtggaccga tctgcgcacc gcccctccgg
     1201 tcggcgacgg gtattactac ctcgtgcggg gcgtcaagtc gtgcggcgcc gggtcattcg
     1261 gccccgggct cgccggcccc gacaccctgc gctgctcccc ctaggagatc gagcaccggt
     1321 gccacgaggg ccgtgcccgc ggcctcgtgc gctgccgggt tccagtgccc gacgccgacg
     1381 ttgatccacc gcttcgggtc gatccggccc tcgacgagga acgcgggggt caggtcgaag
     1441 aaccggaacc cgtggcggct cgccagatcg gcgaaccgcc gcgccggcag ggacacgtcc
     1501 ccgccccccg gcacgtcggc caggagcgcc cggaaatgcc gtgacacgat cggctggtgg
     1561 acgaggagga acgggatccc ctcgtccctg cagaagcgcg ccatctccag gatgcagccg
     1621 tcgaagacgc gccaggcctc cacccagtcc ggtggcgggg tgcgcgcgta ggtcatcgtc
     1681 cagggctcgc cccgggggcc gccgtccgtc cagatcgcgc gccacagtcc gaccgcgttg
     1741 aggttgatgg cgacgtagcg cgcgagggcg gaattcagca ccagccgcac tttccggttg
     1801 ggcgcgtgcg gccggggcgg acgatgcgcc aggtgtccgg cgtcgtcgaa gtagaagcag
     1861 gggccttcgg cacccctgga caccgcgcag gcggtgtccg acaggtcgtt cgacacccac
     1921 agcaggatga cgaggtcggg gcggtcccgc accacttcgt tctggagcag caagagctcc
     1981 tgtccagtgc cgtagccgct cacggcatag gtgaagacgt cggccggctc acctgcgccg
     2041 cgcagcccgg cctccagcac gtcgaagaag cgtcggcccc gatcgatgta gagggcctcc
     2101 acgtaggaat cgccgagcac cgcgacgcgg gcgtggccgg gggtgcgctc cacggtgtac
     2161 tcgtgctcgt tgttgaaccc cgagccgttg atccggtacg tggcgtggat cccttcgccg
     2221 aaccacgtcc cgctctggtt gggcgccagc cggtatccga ccaccggatc cccttcgtgc
     2281 caggggatgt ccgccaccgg caggaacagc cggaccgcga cctcgagcag gaggaagagt
     2341 gtcgcgaggc tgccgacgac gaggagcgcg ttggcagcgg cctccctggc ccgagcgctc
     2401 atcgtccggg ggccgtctcc cggggcgcac gccccacgac gacgacgcgc agccgctccg
     2461 gggcgagcat gcgctccgcg gcggcggtga cgtccgaccg actcagtcgc gcggcgcggt
     2521 ccgcctcgtg cgccaccgcg cgatcatcct cgccccgcag cagggcggcg agccacaggc
     2581 gcgccgcctg ctcggggtcc gcgcgccgca cggccaggcg tccggcggtg aacgacttga
     2641 acgcggcgat ctcctcgtcg ccgatgtcgc ggcgcgcgcg ggcgagttcg gcgacgagcg
     2701 cttcctcgag ccggccgagc gcgtccggat cggcccccat cccgaaccag acgagcggcg
     2761 ctccgcggcg gaccgactcg ctgtccatgt cgtagacgag ccccatctgc gcgatggcgc
     2821 gcgtgccgag gcggccgctg tagttgtgct gcaggatcca ggcgaccgcc tcctgcgcca
     2881 tggcgtcggc gtcgccggga atggcgagta cgagcgcccc ctgcgggatg tcgcggatcg
     2941 ccgagatgtc ccgccccggc tgcagccccg ccgccgaggc ccgccgcgtc ggcggcgcgc
     3001 cccccagcgg tgcggcgaag ctcgcgaggc gatccttgac cgctcccggg tcgaacgggc
     3061 cgacgagcgc gaagaacggc ccggagggcc gtccggaggc ccccggcgac gaggaggtgg
     3121 cggcggggcc ggcggcccga tcgaggtagg ccatcgcgcg ccgcatcgga cgggacaggc
     3181 tctccggatc gacggtggac gggcccggtt tcggcttgga cagcttcaga ccgcgcgact
     3241 ggagcatccc gatcgcgtcg tcgaaggcgc cggccgggcc ggccatctcc aggagcacgg
     3301 tgtcgcgcgc cgcgaacgga ccgcgacccg ggagcgtgac ctcgagcgtc acgccgaggg
     3361 ggacgaaggc gcgggccaga tcgtcttcgt tggcgagcgc ggtcccggcc ggggcggcga
     3421 cggcgaggtg cagcacgaag gtcggggctc cgggacgggc atcgacgatg gcgcgcgcgc
     3481 cgctgccgag accgagggtc ccgatttgag cgcgcgcgac gcgcttcgtg gcgatgcccg
     3541 gcgcgccgat cgggcgcgcc ggcgccgggc gcttcggccg ctcagccgtc gggggcacgg
     3601 cgattccgcc gatcggaccg gagcgggccg ccggcaggac gagaccgatc gccgcccggg
     3661 acggcacgag cgctgcggcg gcggcgcgca cgtcctcggc cgtgacggcg tcgacggcgg
     3721 cgcgcacctg cgagcgcagc tcgagcccgc cgagcgcggt ccagtacgcc atctcgtgcg
     3781 ccttctcgac cagtgagtca gtctccagga gatcggcggc ggcgatgcgg cggcgcgccg
     3841 acgcggcgtc ggcggcatcg gccccgtcgg ccgcctccgc caggagatcg aagagggccg
     3901 cctcggcctc ggacgccttc gccggctcga atcccgaggc gtacagggtg aagtgatatg
     3961 ggtacaaggt cgggacgagc gaggcgccgg cctcctcgag gagccccgtt tcgaccagcc
     4021 ggccgagcgg tgaaccggcc ggggcgcccc cgccggtccc gaggtccccc gagtgccggt
     4081 agaacgacag gccggcggcg ttcccgatga gcgcctcgag caggagcagc gcggcgaact
     4141 cgcgcggccg cgacggtggc ggggcgagaa aggagatctg cagcgccggc gtcgccccgg
     4201 cgcgcaggag cgtcagccgg cgggggccct cgagcggcgg ctcgaccgtc cggatggtgg
     4261 tgtcggcgcc ccccttcggg atcgggccga agatctcctc gatcgcgcgc cgcgccgccg
     4321 cggcgtcgat gtcgccggcg atcgccagga ccgcgttccc cgagccgtag taccggcgat
     4381 agaaggcgac gagatccgca tgggtgatgc cggcgaggtc gctctcccag cccatcgtgt
     4441 tgttgcgata gggatgctgc tcgaagagga gcgcggtggt cgcgtcgaac aggtcggagc
     4501 gcgcatcgag ctggtagccg cggtactcct ggaagacggc gccgcgctcc ggctcgacct
     4561 cgtccgctgc catgcggccg gagaccatcc gctcggcttc gagacgcagg agcgtcgcca
     4621 gcgtctcttt cggcgccgcc tggaaatagg tcgtgcagtc gagggtcgtg tagccgtgcc
     4681 actccccgcc ggcccgctcg atcaccccgg tgacgtccgt gagcccgaat cgctcggtgc
     4741 cgcggaagag catgtgctcg aggtaatgcg cgatgccggt gatgccgatc gtctcgttgc
     4801 gcgccccgac ccggtagagc atctgcaccg cgacgaccgg gtgcgcggcg tcggggcgca
     4861 gcacgcaggt caggccgttg tcgagggtga aggaggtggc gccggcgagc gggtcggcct
     4921 ccggcggcgc cgccggggcg gccccgagca ggaacgccgc gccgaggagg agcggcacga
     4981 ccgggaggag gacaggcgag aaggcccggg ggcgcggcat gcggagaggc atcgtaccag
     5041 aggggggagc cgctccgtgt acaatgccgc tcctgtgggg gtgagcatgg aagtgagaag
     5101 aggccgcgcg cgtcggctgg cgccggttct gatggcggtg gcgctctgcg gcctgaccgc
     5161 ctgtgaagac gaaggggat
//
LOCUS       DASRIQ010000642         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2598984, whole genome shotgun sequence.
ACCESSION   DASRIQ010000642 DASRIQ010000000
VERSION     DASRIQ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2598984"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VFD06_15950"
     CDS             <1..1229
                     /locus_tag="VFD06_15950"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZN05078.1"
                     /translation="VASLILWGPPGSGKTSIARLIAERSRARLVAYSAVLSGIREIKD
                     VMNAAERSLLAGDRRTLLFVDEIHRFNRAQQDAFLPHVERGTITLIGATTENPSFSIN
                     AALLSRCAVHVLEPLGEEDLGRLLRRALERDDELAALRLDVAEAELRLLASRSGGDAR
                     RAFNLLEAVALRAGTLPEGRRRVTRADVERGIHRAALLYDREGEEHYNLISALHKSLR
                     NGDADAAIYWLVRMLESGEDPLYIARRLIRFASEDVGNADPGALRIALSAREAFEALG
                     MPEGALALVQAAAWLAAAPKSNAVTAAHGKVLEDLREGRTDPVPHAIRNAPTRLMREL
                     GYGRGYVSAHDTEEGFAFLECLPERLRDRRYYDPKPVGREKEIGERLEHQRRARAARR
                     AGDRPATTPVREDPAE"
     gene            complement(1195..1395)
                     /locus_tag="VFD06_15955"
     CDS             complement(1195..1395)
                     /locus_tag="VFD06_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05079.1"
                     /translation="MNSSKTWKSGERCQRPGLYRCQQCRLEGRETTRVMAIAAVFPMC
                     DVCPGGDATWRLIRPDPPERAS"
     gene            complement(1457..1708)
                     /locus_tag="VFD06_15960"
     CDS             complement(1457..1708)
                     /locus_tag="VFD06_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05080.1"
                     /translation="MTRSLDRTIPFPKLFARRTRLSRVPSMLRPGARAARGVVPDQSA
                     PTESGKGGECHRQEKEGREEKGEEESKTLNTLWNGNPEG"
     gene            complement(1850..2722)
                     /locus_tag="VFD06_15965"
     CDS             complement(1850..2722)
                     /locus_tag="VFD06_15965"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN05081.1"
                     /translation="MRMAGSGLWTGVAGACVVSGAVLCVDAVLVEPDWIEVTHHLEYV
                     SSWKRSAPDVVIVHLSDPHVEGMGRRERRALEAVAGARPDLVAITGDFVRSGRDVTSL
                     QRFLAGLPARRTFAVWGNHDHASGLATSAGADLLRAAGITLLDNAHRRLATAAGPIVV
                     AGIDDPSSGLDNLGRALAGVARRDLTILLSHSPEIAGALGNRDVDLMLSGHTHGGQVR
                     LPLLEAVLLPPGTRMVQDGWFDADGGARLHVSRGLGWCGLPIRFLCRPRVDVITLRGG
                     APPVPPHRRRSNAV"
BASE COUNT          462 a         1162 c         1130 g          409 t
ORIGIN      
        1 gcgtcgcctc cctgatcctg tggggccccc ccggaagcgg caagacctcc atcgcccggc
       61 tcatcgccga gcggagccgc gcgcggctgg tcgcctacag cgccgtgctc tccggcatcc
      121 gcgagatcaa ggacgtcatg aacgcggcgg agcgctccct cctggcgggg gaccgccgca
      181 ccctcctgtt cgtcgacgag atccaccgct tcaaccgcgc gcagcaggac gccttcctgc
      241 cgcacgtgga gcgcggcacc atcaccctca tcggcgccac caccgagaac ccgtccttct
      301 cgatcaacgc cgccctcctg tcccgctgcg ccgtgcacgt cctcgagccg ctgggtgagg
      361 aggacctcgg gcgcctgctg cggcgggcgc tcgaacggga cgacgagctc gccgccctgc
      421 gcctcgacgt tgcggaggcc gagctgcgtc tcctcgcctc gcggtcgggc ggcgacgccc
      481 gccgcgcctt caaccttctg gaggcggtcg cgctgcgcgc cgggacgctc ccggaggggc
      541 gccggcgcgt gacgcgcgcc gacgtcgagc gcggcattca ccgcgccgcg ctcctctacg
      601 accgcgaggg ggaggagcac tacaacctga tctcggcgct gcacaagtcg ctccgcaacg
      661 gcgacgccga cgcggcgatc tactggctgg tgcgcatgct cgaatcgggg gaggacccgc
      721 tctacatcgc gcgccggctc atccgcttcg cgagcgagga cgtcggcaac gccgacccgg
      781 gggcgctccg catcgcgctg tcggcgcgcg aggcgttcga ggcgctcggg atgccggagg
      841 gggcgctggc cctcgtgcag gcggccgcct ggctcgccgc cgcgccgaag agcaacgccg
      901 tcacggcggc ccacgggaag gtgctggagg atctgcggga ggggcggacc gacccggttc
      961 cgcacgcgat ccgcaacgcg ccgacgcggc tgatgcgcga gctggggtac ggccgcggct
     1021 acgtctcggc gcacgacacc gaggagggat tcgcctttct cgagtgcctg ccggagcgcc
     1081 tgcgcgaccg gcgctactac gatccgaagc cggtcgggcg ggagaaggag atcggcgagc
     1141 ggctcgagca ccagcgccgg gcgcgcgcgg cgcggcgcgc cggcgaccgg ccggctacga
     1201 cgcccgttcg ggaggatccg gccgaatgag ccgccaggtc gcgtcgcctc cggggcagac
     1261 gtcgcacatc gggaacaccg ccgcgatcgc catgacgcgc gtcgtctcgc ggccctcgag
     1321 gcggcactgc tggcagcggt acagccccgg ccgctgacag cgctcgccgc tcttccacgt
     1381 cttcgaggag ttcatccgcc gcatcctccg cctgccgccg gaccgggggg cggaaaaaaa
     1441 tgaggggtga cggccgtcac ccctcgggat taccgttcca cagcgtgttc agcgttttgc
     1501 tttcttcttc ccctttttct tcgcggcctt ctttttcttg gcgatgacac tcaccccctt
     1561 tcccggattc agtcggtgcg gattgatcgg ggacgactcc ccgcgcggcg cgcgcgccgg
     1621 ggcgaagcat cgacggaacg cgcgacaagc gcgtgcgtcg tgcgaacaac ttcgggaacg
     1681 gaatggttcg atcgagggaa cgcgtcacga gcgagaacga tcgtgtcgtt catcagacgc
     1741 gattctacgg ggacgatcac agggtgtcaa tacatccacg ccgcgatcgt cgcgcgatcg
     1801 ctgcgcgagg cggcgcgggg gatcgatccg cgcgcggctg cggcgcgcct cagacggcgt
     1861 tcgatcggcg acggtgcggt ggtacgggcg gcgcgccacc gcgcagcgtg atgacgtcca
     1921 cccgcgggcg gcagaggaag cggatcggca gaccgcacca tccgaggccg cggctgacgt
     1981 gcaggcgcgc gccgccgtcg gcgtcgaacc atccgtcctg aaccatgcgc gtgccgggcg
     2041 gcagcaggac cgcctcgagc agcggcagcc ggacctggcc accgtgcgtg tgccccgaga
     2101 gcatcagatc gacgtcgcga ttgccgagcg cgccggcgat ctcgggactg tgcgacagga
     2161 ggatcgtcag gtcgcggcgc gccacgccgg ccagcgcccg cccgaggttg tcgagaccgc
     2221 tcgacggatc gtcgatcccg gcgacgacga tgggcccggc cgcggtcgcg agccggcggt
     2281 gggcgttgtc gagcagagtg attcccgccg ccctcaagag atccgcccct gccgaggtgg
     2341 cgaggccgct cgcgtgatcg tggttccccc acaccgcgaa ggtccgacgg gccggcagac
     2401 cggcgaggaa gcgctgcagc gaggtcacgt cacgtccgct ccgcacgaaa tcgccagtga
     2461 tcgccaccag atcgggacgc gcccccgcga ccgcctcgag ggcccggcgc tcgcgccggc
     2521 ccatcccctc gacgtgcggg tcgctcaggt ggacgatcac gacgtccggc gcggagcgct
     2581 tccaggacga cacgtactcc aggtggtgcg tcacctcgat ccagtccggc tcgacgagga
     2641 ccgcgtcgac gcacagcacc gccccggaga ccacgcacgc ccccgcgacg ccggtccaaa
     2701 gccccgatcc ggccatcctc atggcggggc ggaatataag gatgcggcct gcctgtccgc
     2761 cagcgccgga cttgacccgg cagggcggat cacgctacct tttgtgcgcc cgccatgtga
     2821 ggggcccgtt ctgggccccc cttcgggaac ggccagaaac ccttgacgga cgaagagcga
     2881 cccggccacg tccggcgccc tgaaactggg gcaaaccgac caggcccggt ccacggacaa
     2941 gaatcgaagg atcgcgagac gatccctgcg cccaccccgg attcctatgg gggggcggga
     3001 ggccccatac cgcacggcaa cggctcggcg ccggtcgccg gcgccgaggg ggggccggga
     3061 cagggacgtc ggcggcgacg gcgcggccgc cgctggcggc gcgggcgcga tcgcggcggc
     3121 gcggcaccgc aggcctcgac accgggcggc gccgctccgc agc
//
LOCUS       DASRIQ010000643         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_1037987, whole genome shotgun sequence.
ACCESSION   DASRIQ010000643 DASRIQ010000000
VERSION     DASRIQ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037987"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..601
                     /locus_tag="VFD06_15970"
     CDS             80..601
                     /locus_tag="VFD06_15970"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HZN05082.1"
                     /translation="MSKFPLVVALVAAATVVTAEARGWAGRGGGRPGPPAEISRELFP
                     PELVMRHQEEIGLSEDQRSKLVHAMQEMQSDLVPLQFEMGQAAAKLRETLAVPRIDEA
                     KAGDLADRLMALETKIKRRHLTTMIQIKNVLTPEQQERLRALREQDRAARRDGRRGDR
                     PSPPDEDGAPPPE"
     gene            complement(603..>2146)
                     /gene="purL"
                     /locus_tag="VFD06_15975"
     CDS             complement(603..>2146)
                     /gene="purL"
                     /locus_tag="VFD06_15975"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZN05083.1"
                     /translation="SEAKRPTVQVGDPFTEKLLLEACIEVMKEKGLVVGIQDMGAAGL
                     TCSTLEMAARGRSGMEIDLDLVPLREEGMTPYEIMLSESQERMLLVATPSTEQRVREI
                     YEKWDLEAQVIGHVNDSGRVRLMSGGRLVADVPAAALADDGPRYDRPQARPAWQDEVQ
                     RIALERIPEPSNLGDVLARLMTSPNLCSRESIYGVYDHMVRTNTVVRPGSDAAVVRLK
                     GSVKALALTSDCNARYCYLDPEEGARLAVAEAARNLACAGAFPIGTTDCLNFGNPERP
                     EIMWQFARAIDGIADACLALEAPIVGGNVSFYNETEGKAIHPTPMIGMVGLLEDVRKS
                     MTQWFKREGDAIYLLGDPSSASLAASEYLALVHGVEAGRPAAVDLKKEKALHDALRAA
                     IAADLVASAHDCSEGGLAAALAECCITNPAGAIGARVALEGNGRADALLFGEAPSRII
                     VSTTTENASRLETILGGADVPFHRLGEVGGPSIEVRLSGNALSLNAADLRRAWQTALP
                     ELLNR"
BASE COUNT          308 a          759 c          761 g          318 t
ORIGIN      
        1 ggctgggaac cggcgcggag accctgtcgg gcgatcccac ggacctgttg aacgagtcca
       61 acgagatgga ggtgttcgaa tgagcaagtt ccccctcgtc gtcgccctgg tcgcggccgc
      121 gaccgttgtc accgcggagg cccgcggctg ggcgggccga ggcggcggcc gtccgggccc
      181 gccggcggaa atcagccgcg agctgttccc tccggagctg gtcatgcgcc atcaggagga
      241 gatcggcctc agcgaagacc agaggtcgaa actggtccac gccatgcagg agatgcagtc
      301 cgatctcgtg cccctgcagt tcgagatggg acaggctgcc gcgaagctcc gcgagaccct
      361 ggccgttccg cgcatcgacg aggcgaaggc cggtgacctc gcggaccggc tcatggctct
      421 cgaaacgaag atcaagcggc gtcacctgac cacgatgatc cagatcaaga acgtcctgac
      481 gcccgagcag caggagcgcc tgcgcgccct gcgcgagcag gaccgggccg cacggcgcga
      541 cgggcgacgc ggcgatcggc cgtcccctcc ggacgaagac ggcgccccgc ctccggagtg
      601 agctagcggt tgagcagctc cggcagcgcc gtctgccagg cgcggcggag gtcggcggcg
      661 ttcagggaca gggcgttgcc gctcaggcgc acctcgatgc tcgggccgcc gacttcaccg
      721 aggcggtgga aggggacgtc ggcgccgccc aggattgtct ccagccggga cgcgttctcg
      781 gtggtcgtgg acacgatgat gcgtgacgga gcctcgccga agagcagcgc atccgcccgg
      841 ccgttcccct ccagcgcgac gcgcgccccg atggcacccg cggggttggt gatgcagcac
      901 tccgccagcg cggccgcgag gccaccctcc gagcagtcgt gcgccgaggc gacgaggtcg
      961 gcggcgatcg ccgcgcgcag cgcgtcgtgc agcgccttct ccttcttgag gtccaccgcc
     1021 gccggcctcc cggcttcgac gccgtgcacc agcgccaggt actcgctggc ggccaggctc
     1081 gcgctcgacg gatcccccag caggtagatg gcgtcccctt cccgcttgaa ccactgggtc
     1141 atcgatttcc gcacgtcctc gaggagcccg accatgccga tcatcggggt cggatggatc
     1201 gccttcccct cggtctcgtt gtagaacgac acgttgccgc cgacgatggg ggcctcgagc
     1261 gccaggcacg cgtcggcgat gccgtcgatg gcgcgggcga actgccacat gatctcgggg
     1321 cgctcggggt tgccgaagtt caggcagtcg gtcgtgccga tcgggaaggc gccggcgcac
     1381 gccaggttgc gtgccgcttc ggcgacggcg aggcgcgccc cctcttccgg gtcgaggtag
     1441 cagtagcgcg cgttgcagtc gctggtcagc gcgagcgcct tgacgctccc cttcagccgg
     1501 acgacggcgg cgtcggagcc ggggcgcacc acggtgttgg tgcggaccat gtggtcgtag
     1561 acgccgtaga tcgactcgcg cgagcacagg ttgggcgagg tcatcagccg cgccagcacg
     1621 tcgccgaggt tcgacggctc gggaatgcgc tcgagcgcga tgcgctgcac ttcgtcctgc
     1681 caggcggggc gcgcctgcgg gcggtcgtag cgcggcccgt cgtcggccag cgccgcggcc
     1741 ggcacgtcgg cgaccagcct cccgcccgac atgaggcgca cgcggccgct gtcgttgacg
     1801 tggccgatga cctgcgcctc gaggtcccac ttctcgtaaa tctcccgcac gcgctgctcg
     1861 gtcgaggggg tggcgacgag cagcatccgc tcctgcgact cggagagcat gatctcgtac
     1921 ggcgtcatcc cctcttccct gagcggcacg aggtcgaggt cgatctccat gccgctgcgg
     1981 ccgcgcgccg ccatctcgag cgtcgagcag gtcagccccg ccgcccccat gtcctggatg
     2041 ccgacgacga gccccttctc cttcatcact tcgatgcacg cctcgaggag gagcttctcg
     2101 gtgaacgggt cgccgacctg gacggtcggg cgcttcgcct ccgacg
//
LOCUS       DASRIQ010000644         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2340424, whole genome shotgun sequence.
ACCESSION   DASRIQ010000644 DASRIQ010000000
VERSION     DASRIQ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340424"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFD06_15980"
     CDS             complement(<1..251)
                     /locus_tag="VFD06_15980"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HZN05084.1"
                     /translation="MEHAELFEKMAQSVIAGEADDSAALAHKALELGIRPLDAIDEGF
                     VKGIRVVGDRFGAGELFLPELVMSAEAMKAALAILEPELA"
     gene            complement(305..1180)
                     /locus_tag="VFD06_15985"
     CDS             complement(305..1180)
                     /locus_tag="VFD06_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN05085.1"
                     /translation="MAQDSAITGSKKRAERGLGMVSGNQAVRRAIAVLKAFSDETPDM
                     GVTEVSRKVSLHKSTVYRLLSAFEDEGLIARNPESGKYRLGPELIVLGEQVLRHTEVH
                     RVALPFLRELAERTGETIDLEVLSGGNVVTIEEIAGKHVVAATGAIGMPWAAHATSNG
                     KVLLAHQPTEKQRQILSRTLRRFTARTLTDAKAVARELAKVREQGYALSFGELEEHLV
                     AIGMPIRGRSGDAVAAVSISGPDTRLTPDKLTALLRLGLETCARISSRLGYHGPSLPR
                     PLGAKPAGPRPVVVS"
     gene            1579..2220
                     /locus_tag="VFD06_15990"
     CDS             1579..2220
                     /locus_tag="VFD06_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05086.1"
                     /translation="MRVGVLLAGCGPLDGSDPGETLLLLLALEEAGERPVILATDRPL
                     ERVVDHRTALADETGARSALVESARLTRHPVRDLREVAPEDLEALFVPGGYGAALNFQ
                     GGFARPGESRPLHPEVERFLRHFESARKPIGTIGLGEIPVRALYGLDLPADPSGPPGQ
                     VDVDAARRLWHTPGAVSGGRLPDLRAGITALVAAVLEAMRQGDPEARASRRAS"
     gene            2217..2459
                     /gene="moaD"
                     /locus_tag="VFD06_15995"
     CDS             2217..2459
                     /gene="moaD"
                     /locus_tag="VFD06_15995"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HZN05087.1"
                     /translation="MTIRIWLFARYRELADVTALDLEVAPGTTLGALWTMVQRRVPAL
                     AQESRPLMAIDQTYAQPDQVVRPGSEVAFFPPVSGG"
     gene            2456..2947
                     /locus_tag="VFD06_16000"
     CDS             2456..2947
                     /locus_tag="VFD06_16000"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZN05088.1"
                     /translation="MSAAPEAAASYCRLGPEPIDARALEARVMGSDRGAIATFAGVVR
                     DHHEGRPVRRLEYTAYEPMAERVLADIVREAAQRFGTPHIALQHRLGPLAVGETSVVV
                     AAAATHRHQALAACAWVIDEVKARAPIWKKEFGDDGVFWIEGPGSCRSQEPEKPAGGA
                     GTA"
     gene            3335..>3638
                     /locus_tag="VFD06_16005"
     CDS             3335..>3638
                     /locus_tag="VFD06_16005"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HZN05089.1"
                     /translation="MTEVLQAVLPAGVIALGGGLLFGWFLKGRAARVEVARTRGQSDE
                     ALHRTRREAEDQKRQALLAAREEWLTTKGRLELEIRARSQEVQQSHRALEEREAAHR"
BASE COUNT          489 a         1312 c         1291 g          546 t
ORIGIN      
        1 gccagctccg gctcgaggat ggcgagcgcc gccttcatcg cctcggcgct catcaccagc
       61 tccggcagga acagctcgcc ggcgccgaaa cgatcgccga ccacccggat ccccttgacg
      121 aacccttcgt cgatcgcgtc gagcggcctg atgccgagct cgagcgcctt gtgagcgagc
      181 gccgcggagt cgtccgcctc gccggcgatg acgctctggg ccatcttttc gaacagttcg
      241 gcgtgttcca cgattgctcc ctcctcccag gtcctgcgaa atcagcccct cggggggcgg
      301 gttgtcacga caccacgacc ggacgcggac cggccggctt cgcaccgagc ggccgcggca
      361 gggacggccc gtgatagccg aggcgcgacg agatgcgcgc gcaggtctcg aggccgagcc
      421 gcaggagcgc ggtcagcttg tcgggggtca gacgcgtgtc cggacccgag atgctcaccg
      481 ccgcgaccgc gtcgccgctg cgcccgcgga tcggcatgcc gatggccacc aggtgctcct
      541 cgagctcgcc gaacgacagg gcgtaaccct gctcgcgcac tttcgccagc tcgcgcgcca
      601 ccgccttggc gtcggtcaac gtgcgcgcgg tgaagcggcg cagggtgcgc gacaggatct
      661 gccgctgctt ctcggtgggc tggtgcgcca ggagcacctt gccgttgctc gtggcgtgcg
      721 ccgcccacgg catgccgatg gcgccggtcg cggcgacgac gtgcttcccg gcgatctcct
      781 cgatggtgac gacgttgccg cccgacagca cctcgagatc gatcgtctcg ccggtcctct
      841 cggccagctc gcgcaggaag gggagggcga cgcggtgcac ctcggtgtga cgcagcacct
      901 gctccccgag cacgatgagc tccggcccga ggcggtactt gccgctctcc ggattgcggg
      961 cgatgagccc ctcgtcctcg aaggccgaga ggagccggta gacggtgctc ttgtgcaggc
     1021 tcaccttgcg gctcacctcc gtcacgccca tgtccggcgt ctcgtcgctg aacgccttga
     1081 gcaccgcgat ggcgcggcgc accgcctggt tgccgctcac catgccgagc ccgcgctccg
     1141 cccgcttctt gctgcccgtg atcgccgagt cctgagccat gaccgccgtc ccttcttcgc
     1201 ctggcttccc gcctgtctgt ggcgaatatg cgtatggaac attgtgccgg acaggaaggc
     1261 acgccccgat tgtccgtcga ggcccccctg atccgcaacc actttctggc tacaaatgac
     1321 gtaacggcaa cgctttacga acttccggcc gcgtctgagg ccgtaacagc atcgccgtcc
     1381 cgatcccgcc tggacccgac tccgcggccc cggaccaatg gtgcggcagc aagacacggt
     1441 ccgcggcatt cttccgatcc ggcctccggc ttgaaaccac attgagaaac gaggctgcgt
     1501 aatgcgcgac aacgataccg cgagggaagg agtccgtcaa ccacgatgcg ggacggtctc
     1561 cttcttataa tgcgtggcat gcgcgtcgga gtgctcctgg ccgggtgcgg gccgctcgat
     1621 ggaagcgatc ccggggagac cctcctgctc ctgctggctc tggaagaggc gggggagcgg
     1681 ccggtgatcc tcgccacgga tcgacctctc gagagggtcg tcgatcaccg caccgcgctg
     1741 gcggacgaaa ccggcgcgcg cagcgcgctt gtcgagtcgg cgcgtctgac gcggcatccg
     1801 gtgcgcgatc tgcgcgaggt cgcgccggag gatctcgagg cgctcttcgt cccgggtggc
     1861 tacggcgccg cgctcaactt ccagggcggc ttcgcgcgtc cgggcgaatc ccggccgctg
     1921 catccggagg tcgagcgatt cctgcggcac ttcgaatccg cgcgcaaacc gatcgggacg
     1981 atcggtctcg gggagatccc ggtgcgcgcc ctgtacggcc tcgatctccc ggccgatccg
     2041 tccggccccc cgggtcaggt ggacgtggac gcggcccggc gtctgtggca cacgccgggc
     2101 gccgtctccg gcgggcggct cccggacctg cgcgccggca tcacggcgct ggtcgcggcg
     2161 gtgctcgaag cgatgcgcca gggggacccg gaggcgcgcg cctcgcgacg ggcgtcatga
     2221 cgatccgcat ctggctcttc gcccgctatc gtgagctcgc tgacgtgacc gccctcgacc
     2281 tcgaggtcgc gcccgggacg accctcggag cgctctggac catggtgcag cggcgggtgc
     2341 cggcgctggc gcaggaatcg cgccctctca tggcgatcga ccagacgtac gcgcagccgg
     2401 accaggtcgt ccggccgggc agcgaagtgg ccttcttccc cccggtgagc ggcggatgag
     2461 cgcagccccg gaggccgccg cgtcctactg ccgcctggga ccggagccga tcgacgcgcg
     2521 ggcgctcgag gcgcgggtga tgggatccga tcgcggggcg atcgccacct tcgccggggt
     2581 cgtccgcgac caccacgaag gacgcccggt gcggcggctc gagtacacgg cctacgagcc
     2641 gatggcggag cgggtcctcg ccgacatcgt ccgggaggcg gcgcagcggt tcgggacgcc
     2701 gcacatcgcc ctgcagcatc ggctcggccc cctcgccgtc ggcgagacga gcgtcgtcgt
     2761 cgccgccgcg gcgacgcatc gtcaccaggc gctcgccgcc tgcgcctggg tgatcgacga
     2821 ggtcaaggcg cgggcgccga tctggaagaa ggagttcggc gacgacggcg tcttctggat
     2881 cgaaggcccc gggtcctgcc ggtcgcagga gccggagaag cccgcgggcg gtgcggggac
     2941 cgcctgaaga gccgcctttg cggcccccga aatcgcggtg ctatactgcg gcttccggag
     3001 cggccgcagg ccgcgccggg acaacaggtc cggcggtggc cggacgaggt gggctcgttc
     3061 tccggggggt cgatggttca ggtcctgctc gcgctgggtc tcctcctccc gatcgtcttt
     3121 ttcgcggatc tccaaccgtt gaatcgagag ttcggcgcga cgcgcgtccc gaggcggctt
     3181 ccgctcccgg tgcgcgcgag tcgccaccgt ccgcacccgc acctccgggg cgcctccggg
     3241 gcgcctccgg gccgcgggcg ctgacctcgc ttcgcggccg cggttccgcc cccaccccgc
     3301 ccgccgtcgc cggcccagtg ccggagacgg atcgatgact gaagtcctgc aggcagtcct
     3361 tcccgccggg gtgatcgcgc tcggcggcgg gctgctgttc ggctggttcc tgaaggggcg
     3421 ggccgcccgg gtggaagtgg cgcggacccg cgggcagtcg gacgaggcgc tccaccggac
     3481 ccgccgcgag gcggaggacc agaaacgcca ggcgctcctc gcggcgcgcg aggagtggct
     3541 cactaccaag ggacggctcg agctggagat ccgtgcccgc tcccaggagg tccagcagtc
     3601 gcatcgggcg ctcgaggagc gcgaggcggc gcaccggg
//
LOCUS       DASRIQ010000645         9350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_500899, whole genome shotgun sequence.
ACCESSION   DASRIQ010000645 DASRIQ010000000
VERSION     DASRIQ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500899"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1524)
                     /locus_tag="VFD06_16010"
     CDS             complement(82..1524)
                     /locus_tag="VFD06_16010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN05090.1"
                     /translation="MPFSKLHLDAALLRAVKEMGFLRPTPIQEQAIPPAATGRDLIAC
                     ASTGSGKSAAFLLPILHRLAPKPRGATRALVLTPTRELAAQIHEHLEELAVHTPVTGA
                     AVFGGVGMGSQEHALRSGVDVIVATPGRLLDHMSRPYARLASLEILVLDEADRMLDMG
                     FLPDIRRILKALPHKRQTLLFSATIPDAIASLARELMRDPVTVRLERKSVPAAGIRQA
                     VYPVPRELKSNLLKELLARDLVADAIVFTRTKHRANRLAEFLERHGVPTGRIHGNRSQ
                     TQRTAALAGFKNGRHRILVATDIVARGIDVEALGHVINFDVPADPDSYIHRVGRTARA
                     EQQGDAFTFVAPDEESDLRAIERALGKALPRVTLPEFDYAKRTSERLEVPLGQRLAQH
                     RGGGRRPQGHGGRPQGHGGGGHGQGGRTHGGGGHGRPAAGRRSGGHGRPQGRSGADAR
                     MQSILDRHAPVSEGDGAKTSPGGFRGRRRR"
     gene            complement(1569..2669)
                     /locus_tag="VFD06_16015"
     CDS             complement(1569..2669)
                     /locus_tag="VFD06_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05091.1"
                     /translation="MALCAAAGVVLATASTPAVLLAAAFPVAASAAAVLAAASGQVSI
                     ERPGTASPIAGTIGMRLLAGDTVKVGSGSSATVYLAGGGLVRIPAGSRIEIPREAIPG
                     GGAASAASAGAAGATPPPTEASSLATVMSSRSVEVLEAGLWVLNDPDGSVLLSAMRGE
                     ADDWGATEGGALEPLSPRFETVFETRPRFVWSGPSPARVVVARGKEIVWRGSPSSLGS
                     LTLPADAPELKHGEVYRWWLESDAGKTPPTESVPFRIAVRKAVGEAARFEKEVEGLSA
                     GNDGQGLASLLRCGYYVEVGAWSRVVAAATDLRRSDPGSPAAERALEGSRRQMRLGSE
                     GMSRLAPAAGPTKEAPAAAPAGAPPPTSGSRP"
     gene            complement(2725..3363)
                     /locus_tag="VFD06_16020"
     CDS             complement(2725..3363)
                     /locus_tag="VFD06_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZN05092.1"
                     /translation="MNRPRPLTPERIREAARHLAGRDPDLALLLARHGPPPLWGRPRG
                     FATLVRIILEQQVSLASARAIYGRLVATLGEPTPERLFAAGVDKLRGCGLTRQKAGYC
                     HRLAAAARDGTLDLATVARLDDAAAIDALTRHDGIGPWTAQIYLLMSLRRPDIWPAGD
                     LALVASLRTVKRLRRPPSRERIVAIASAWRPYRAVAARMLWQNYLARPTVVP"
     gene            complement(3360..5183)
                     /locus_tag="VFD06_16025"
     CDS             complement(3360..5183)
                     /locus_tag="VFD06_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN05093.1"
                     /translation="MSPDTDDAPGGSSPTPEPTRSLGRTGEAAGDRGGRDAPGAGARL
                     GHFRIEQAIGEGGAGRVFVAEDLDIPGRRVALKLIRAGGLTPDLEALRREASALAHLR
                     HPHILVVHEIGASEWGPYLVTELMESGSLAARLRRGPLTEREGLRLARGIASALAAAH
                     ARGLLHRDIKPANLLLGKEDGAVKVADFGLVWRRAGPTGPEAEPGVAAPREAAVGPSA
                     GAGARRGPIPGREQALNRGVMESAGTPPYIAPEVLEGDPPDPAADQFAFGVTLCEMLT
                     GRRPFEGQDWGTKVMSGAPEIPAGMARDVESVVRRAIARRSADRFPAMSDVVAAVERI
                     QAHRDPRRRRLAWTIGASAALFVLLVGGWGLLRFRQGEKVRALNEAGRVALERGDHDG
                     ARRAYLAAHGADPSFLPACTSLGALAARERNPTWAITILEECASTFPAAAEVRYNLGA
                     ALQLTGRAEAAERELRLALELAGKGPMRPPALNELAMALVETGRADEAVLLLEPERPF
                     RADEVEGAILSKTLGLAYVEAGRAGDGAALLRQAIEGPLPAAPQRAALVGLGKALETT
                     GDAEGALEAYSRALLAAEGTGGEADVAVAAAARAGLARLQP"
     gene            5207..8275
                     /locus_tag="VFD06_16030"
     CDS             5207..8275
                     /locus_tag="VFD06_16030"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZN05094.1"
                     /translation="MTVLRSMLLALAFLAGPVTAATAAAAPEPAAAPMDRFDEAVRRQ
                     DLAGALAAAREAAQAVRADRQMGAEERSRRLGEIGIRIVQEVGAGAEAAAAAEDLFQE
                     ALEIRRVRFGDESVEVAESYDLLSTFHFMIGRYDAAEEEERLAVTLREAALPPDDPAI
                     ALARDGLGTILFRQGRYAEAEPLLLDALDTYRKADPPEAESLAGALNTLAELARTRGD
                     LKLAEERLNEGLSYAIAAPALQPTLLNNLAGLFKDQGRYDEAEDLLRRSLQLREAADP
                     PQVAQVATALLNLAELARLQGKSSEAAPLYAQALEAARRGLGADNPELAFFLNQAAVN
                     AEEQANGDEALRLNGEAIDLLRRAPGAETQVAQSLLDRGLILLRTGRAEPAIASVREA
                     LAIRARALGEDHPETATARLALAQALAPGGEANTGAAAGAQEARTEIDRALSVLDAAD
                     VFPDERAEAHAVRARLRRGAGDRDGAVADLQKAAAIVEDLRPRSGGGEAARAGRFARH
                     ATIFDDLALSLFETGRTNDGFDASERGRGRALLDQLETAHVDLRSAIPAAEREALRVR
                     EQEALARLAEARDQLAFTRARSDLEASERDRRVAVLEAELADAARAVADLREEAQNRS
                     PLWRRSGSASPPGLTTVQARLLPRRGFLLLLYVIGPDRSGLLLVPPAPQLAQFHRLTL
                     NAADAAALGLPEGAVGAQALARAMRPLLAALARPPAAAASEWQDEPGGAPGERAPLPA
                     RLNALWRVLLPVDVWNRVRESDEVIVLPDGPLAVLPFEALVVKPAKRLEDSIFWIDDG
                     PAIRYAPSAGVQRALADRAPAGSGLLSVADPAYRTLPRLPGTEAESRSVVQASRRKEL
                     GASPAVVLTGAAATESAVRAALPGKKFLHLAVHGKVEPRLGDLFASLALADDEALMLF
                     EIYDLDLQAELAVLSACETSRGRMVEGEGAFALSRGFLAAGARRVVASLWSVDDASTA
                     ELMRTFFRRLGPGDDRSYAAALRDARRVLRGKGKWKSPFYWAPFLLTGAY"
     gene            complement(8330..>9350)
                     /locus_tag="VFD06_16035"
     CDS             complement(8330..>9350)
                     /locus_tag="VFD06_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HZN05095.1"
                     /translation="LDPTLDIYGVRITYTPPASPSATVSDAVAAVPFLPEQTSDSRLP
                     FQGTLAGQYRFNTGLQLPDGAQVLSVSMSILDNSDDGDMTASLYRTDSTSSGAGNTLM
                     ATLTSSGAAGGVRTFTTDVIRGRVIDNDHYFYYAQFWTGFEPVTALGFVVNTAPCGDR
                     DGDGFDGCVNDCNDNRAATRPGAPEICDGQINNCSVSFWPDATVGTIEGDDDFDGFSE
                     CQGDCNDAASSRWSPPSEPRSLMLAHNTGTGVTTLTWTTPLDFGSTTAPVYDTLRSGS
                     PLDFASGAAVCVESNGADLTSTMTGSAPVGGCYYFLIRAEGVCGAGLPGHTSSGAPIS
                     GRNCP"
BASE COUNT         1201 a         3426 c         3540 g         1183 t
ORIGIN      
        1 agagggcggc cccttcgctg ctgcagccga cgatcccgag gtgccggatg aggcgggcca
       61 tgggaagact cggacaggct cctagcggcg gcggcgcccc cggaatcctc cgggggaagt
      121 cttcgcgccg tccccctcgg agaccggcgc atggcgatcg aggatcgact gcatccgcgc
      181 atcggcgccg ctgcgtccct ggggacgtcc gtgtccgccc gatcgccggc ccgccgcagg
      241 gcgcccgtgc cctccaccac cgtgcgtccg gccaccctgt ccgtgccccc caccgccgtg
      301 gccctgcggc cggccgccgt gtccctgcgg ccgtcggccg ccgccgcgat gctgcgcgag
      361 gcgctggccg agagggacct ccaggcgctc gctggtccgc ttcgcgtagt cgaactccgg
      421 cagggtgacg cgcggcagcg ccttgccgag cgcccgctcg atggcgcgca gatcgctctc
      481 ttcgtccggc gcgacgaagg tgaaggcgtc cccctgctgc tcggcgcgcg ccgtccggcc
      541 gacccggtgg atgtaggaat cgggatcggc ggggacgtcg aaattgatca cgtgcccgag
      601 cgcctcgacg tcgatgccgc gcgccacgat gtcggtcgcc acgaggatcc ggtgccgccc
      661 gttcttgaac cccgcgagcg ccgcggtgcg ctgggtctgg ctgcggttgc cgtggatccg
      721 cccggtcggg acgccgtggc gctcgaggaa ctccgccagg cggttggccc ggtgcttggt
      781 gcgcgtgaag acgatggcat cggccacgag atcgcgcgcc aggagctcct tcagaaggtt
      841 tgacttcagc tctcgcggca ccggatagac cgcctgccgg atgccggcgg cggggaccga
      901 cttgcgctcg aggcggacgg tgaccgggtc gcgcatcagc tcccgcgcca gggaggcgat
      961 cgcatccggg atcgtggcgc tgaacaggag cgtctgccgc ttgtgcggca gcgccttgag
     1021 gatcctccgg atgtccggga ggaaccccat gtcgagcatc cggtccgcct cgtcgagcac
     1081 caggatctcg agcgacgcga gccgcgcgta gggccgggac atgtgatcga ggagtcgccc
     1141 gggggttgcg acgatgacgt ccacgccgct ccgcagcgca tgctcctgcg accccatgcc
     1201 gacgccgccg aagaccgcgg ccccggtcac cggcgtgtgc accgccagct cctcgaggtg
     1261 ctcatggatc tgcgccgcca gctcgcgcgt cggcgtcagg accagcgccc gcgtcgcgcc
     1321 gcgcggcttc ggcgccaggc ggtggaggat cggcagcagg aaggcggccg atttcccgct
     1381 gccggtgctg gcgcaggcga tcaggtcccg ccccgtcgcg gcgggaggga tcgcctgctc
     1441 ctggatcggc gtcggccgca ggaaccccat ctccttgacg gcccggagca gtgcggcatc
     1501 gaggtggagc ttcgagaagg gcatgagggg gccacgttag cagatccgcg cgaaaaccgc
     1561 gggatcctct agggacgcga gccggaggtc ggcggggggg cgccggctgg agccgcggcg
     1621 ggagcctcct tcgtcgggcc ggccgccggc gccaggcgcg acatcccctc ggacccgagg
     1681 cgcatctggc gccgcgagcc ttcgagcgcc cgctccgccg cgggggagcc gggatcgctg
     1741 cggcgcagat cggtggccgc ggcgacgacc cggctccacg cgccgacctc gacgtagtag
     1801 ccgcagcgca ggaggctcgc gagcccctgc ccgtcgttcc cggcggagag gccctcgacc
     1861 tccttctcga agcgggcggc ttcgccgacc gccttgcgca cggcgatgcg gaacgggacc
     1921 gactcggtcg gcggcgtctt ccccgcgtcc gattcgagcc accagcggta cacctcgccg
     1981 tgcttcagct cgggcgcgtc ggccggcaag gtcaggctgc cgagcgagga gggggagccg
     2041 cgccagacga tctccttgcc gcgcgccacc acgacccggg ccggcgaagg tccgctccac
     2101 acgaaacggg gccgcgtctc gaacaccgtc tcgaagcgcg gcgacagcgg ctccagcgcg
     2161 cccccttccg tcgcccccca gtcgtccgcc tcgccacgca tggcgctcag gagcacgctc
     2221 ccgtccggat cgttgagaac ccacagtccc gcttccagga cctcgaccga gcgggaggac
     2281 atcacggtcg cgaggctgga ggcctccgtc gggggagggg tggcgccggc cgctccggcg
     2341 gaagcggcgg aggctgcgcc gccccccggg atcgcctcgc gcgggatctc gatgcgactc
     2401 ccggccggga tgcggacgag gccgccgccg gcgaggtaaa ccgtggcgga cgagccgctc
     2461 ccgaccttga cggtgtcgcc ggcgaggagc cgcatgccga tcgtcccggc gatgggggaa
     2521 gcggttcccg ggcgctcgat gctcacctgc ccgctcgcgg ccgccaggac cgccgcggcc
     2581 gacgcggcca cgggaaaagc cgcggcgaga agaacggccg gcgtcgaggc ggtcgccagg
     2641 acgaccccgg cggcggcgca cagtgccatg agagagcgcc gccacggacg gatctctccc
     2701 ggaatcgatg ccgaacgcat acagctaggg taccaccgtc gggcgggcga ggtagttctg
     2761 ccagagcatg cgcgccgcga ccgcgcgata ggggcgccac gcggaggcga tggcgacgat
     2821 ccgctcgcgg gacgggggcc ggcgcagccg cttgacggtg cgcagcgacg cgaccagggc
     2881 gaggtcgccg gccggccaga tgtccggccg gcggagcgac atcaaaaggt aaatctgcgc
     2941 cgtccagggg ccgataccgt cgtggcgcgt cagcgcgtcg atcgccgccg cgtcgtcgag
     3001 gcgcgccacg gtcgccaggt cgagcgtgcc gtcccgcgcc gcggcggcga gacggtggca
     3061 gtagccggcc ttctgacggg tcagcccgca gccgcgcagc ttatcgactc ccgccgcgaa
     3121 cagccgctcc ggcgtcggct cgccgagcgt cgcgacgagc cggccgtaga tggcgcgcgc
     3181 cgaggcgagc gacacctgct gctccaggat gatccggacg agcgtcgcaa acccgcgcgg
     3241 ccggccccag agaggcggcg gtccgtgccg cgcgaggagc aaggcgaggt cgggatcgcg
     3301 ccccgccagg tgccgcgccg cctcgcggat ccgctccggc gtcagcgggc ggggccggtt
     3361 catggctgga gcctcgcgag gccggcgcgc gccgccgccg cgacggcgac gtccgcctcg
     3421 ccgccggtcc cttcggccgc gaggagcgcc cggctgtagg cctcgagcgc cccttcggca
     3481 tccccggtcg tctcgagtgc cttgccgagg ccgacgaggg cggcccgctg cggcgccgcc
     3541 gggagaggac cctcgatcgc ctggcgcagg agcgccgcgc cgtcgccggc ccgcccggcc
     3601 tcgacgtacg ccaggccgag cgtcttcgac aggatcgccc cttcgacttc gtcggcgcgg
     3661 aacgggcgtt ccggctcgag caggaggacc gcttcatcgg cccggccggt ctcgaccagc
     3721 gccatcgcca gctcgttgag ggcgggcggc cgcatcggcc ccttccccgc gagttccaag
     3781 gcgagccgca gctcccgctc cgccgcctcg gcgcgcccgg tgagttgcag cgccgcaccg
     3841 aggttgtagc gcacctccgc cgccgccggg aaggtcgagg cgcactcctc gaggatggtg
     3901 atcgcccagg tcggattgcg ctccctggcg gcgagcgccc cgagactggt gcaggccggc
     3961 agaaacgacg gatcggcgcc gtgcgccgcg aggtaggcgc gccgcgcgcc gtcgtgatcg
     4021 ccgcgctcga gcgccacccg ccctgcctcg ttcagggcgc gcaccttctc cccctggcgg
     4081 aagcggagga gaccccagcc gccgacgagg agcacgaaca gcgccgccga ggccccgatc
     4141 gtccacgcca gccggcggcg gcgcggatcg cggtgcgcct gaatccgctc gacggccgcg
     4201 acgacgtcgg acatcgccgg aaagcgatcg gccgaccggc gcgcgatggc gcgccgcacg
     4261 accgattcca cgtcgcgcgc catcccggcc gggatctccg gcgcgcccga catcaccttc
     4321 gtgccccagt cctgcccctc gaagggacgc cggccggtca gcatctcgca gagggtcacg
     4381 ccgaaggcga actggtcggc ggcggggtcg ggcgggtccc cctcgagcac ctccggcgcg
     4441 atgtatggcg gcgtgccggc cgactccatc acgccgcggt tgagcgcctg ctcgcggccg
     4501 gggatcggcc cgcgccgcgc tccggcgccc gcggaggggc cgaccgcggc ctctcgaggg
     4561 gcggcgaccc cgggctcggc ttcgggcccg gtgggcccgg cgcggcgcca gacgagaccg
     4621 aagtcggcca ccttcacggc gccgtcctct ttcccgagga gcaggttcgc cggcttgata
     4681 tcgcgatgga gcaggccgcg cgcgtgcgcc gccgccagcg ccgaggcgat cccgcgggcg
     4741 agacgcagcc cctcgcgctc ggtgagcggc ccgcgccgca ggcgggcggc gagcgagccc
     4801 gattccatga gctccgtgac gaggtacggc ccccactccg aggcgccgat ctcgtgcacc
     4861 acgaggatgt gcggatggcg gagatgcgcg agggccgagg cctcgcggcg gagcgcctcg
     4921 agatccggcg tcaggccacc ggcgcggatg agcttgagcg ccacgcgacg ccccgggatg
     4981 tcgaggtctt cggcgacgaa gacgcgtccg gcccctcctt cgccgatcgc ctgctcgatg
     5041 cggaagtggc cgaggcgcgc gcccgcaccc ggcgcgtcgc ggccgccgcg atcccccgcg
     5101 gcctccccgg ttcgcccgag ggagcgcgtc ggttcggggg tcggcgacga gccgccggga
     5161 gcatcgtccg tgtcggggct catcctcttt ctataatagc cgcccgatga cggtcctgcg
     5221 gtccatgctc ctggcgctgg cgttcctggc cgggccggtc accgccgcga cggccgccgc
     5281 cgctcccgaa cccgccgccg cacccatgga tcgcttcgac gaggcggtgc gccggcagga
     5341 cctcgcaggc gccctcgcgg cggcgcgcga ggcggcgcag gcggtgcgcg ccgatcgcca
     5401 gatgggcgcg gaggagcgga gccgccgcct cggtgaaatc ggcatccgca tcgtccagga
     5461 ggtcggcgcc ggcgcggagg cggcggcggc cgccgaggat ctgttccagg aggcgctgga
     5521 gatccgccgc gtccgcttcg gcgacgagag cgtggaggtg gcggaaagct acgacctcct
     5581 ctccacgttc catttcatga tcggacggta cgacgcggcc gaggaggagg agcgcctcgc
     5641 tgtgacgctg cgcgaagcgg cgctgccccc ggacgacccg gcgatcgcgc tcgcccgcga
     5701 cgggctcggg acgatcctgt tccggcaggg gcgctacgcc gaggcggagc cgctcctgct
     5761 cgacgcgctc gacacctacc gcaaggccga cccgccggag gcggagagcc tcgccggcgc
     5821 gctgaacacg ctggccgagc tggcgcggac gcgcggtgat ctcaagctcg cggaggagcg
     5881 gctcaacgaa gggctgtcgt acgcgatcgc cgcgccggcg ctgcagccga cgctcctcaa
     5941 caacctcgcc ggtctcttca aggaccaggg gcgctacgac gaggcggagg atctcctgcg
     6001 ccgttcgctc cagctgcggg aggcggccga cccccctcag gtcgcgcagg tggcgacggc
     6061 gctgctcaac ctcgcggagc tggcgcggct gcagggaaag tcgtccgagg cggcgccgct
     6121 ctacgcgcag gccctcgaag cggcgcggcg cggcctcggg gcggacaacc cggagctggc
     6181 cttcttcctc aaccaggcgg cggtcaacgc cgaggagcag gcgaacggag acgaggcgct
     6241 ccgcctcaac ggcgaggcga tcgatctcct gcgtcgagcc cctggcgccg agacgcaggt
     6301 ggcgcagtcg ctcctcgatc gcggcctgat cctcttgagg accggccggg cggagccggc
     6361 gatcgcgtcg gtgcgcgagg cgctggcgat ccgcgcccga gccctcgggg aggaccaccc
     6421 cgagaccgcg acggcccgcc tggcgctcgc ccaggcgctc gcgcccggcg gggaggcgaa
     6481 cacgggggcg gcggccgggg cgcaggaggc gcggacggag atcgatcgcg ccctcagcgt
     6541 cctcgacgcg gcggacgtct tccccgacga gcgggccgag gcgcacgcgg tgcgggcgcg
     6601 gctgcgccgc ggcgccggcg atcgggacgg cgccgtcgcc gatctccaga aggcggcggc
     6661 gatcgtcgag gacctccggc cgagaagcgg cggcggcgaa gcggcccgcg ccggccgttt
     6721 cgcccggcac gcgacgatct tcgacgacct ggcgctctcc ctcttcgaga ccgggcgcac
     6781 gaacgacggg ttcgacgcgt ccgagcgcgg gcgcggccgg gcgcttctcg atcagctcga
     6841 aacggcgcat gtcgatctgc ggagcgcgat cccggcggcc gagcgggaag cgctccgtgt
     6901 ccgcgagcag gaggccctgg cgcgcctcgc cgaggcgcgc gaccagctgg ccttcacgcg
     6961 cgcgcgctcc gacctcgagg cgtccgagcg ggaccggcgg gtggccgtcc tcgaagcgga
     7021 gctggccgac gcggcgcggg cggtcgcgga tctccgggag gaggcgcaga accggagccc
     7081 cctctggcgc cggagtggca gcgcctcccc gcccggcctc acgacggtgc aggcgcggtt
     7141 gctgccgcgg cgcgggttcc tgcttcttct ctacgtgatc gggccggacc gctccgggct
     7201 cctgctcgtc cctcccgcgc cacagctggc ccagttccat cgtctgacgc tgaatgccgc
     7261 cgacgccgcg gccctcggtc tgccggaagg ggcggtcggt gcgcaggcgc tggcgcgcgc
     7321 catgcggccg cttctcgccg cgctcgcccg ccctccggcc gcggcggcgt cggagtggca
     7381 ggacgagccg ggcggcgccc cgggagagcg ggcccccctt ccggcgcggc tgaacgccct
     7441 gtggcgcgtc ctgcttcccg tcgatgtctg gaaccgggtg cgggagtcgg acgaggtgat
     7501 tgtcctgccg gacgggccgc tggcggtcct gccgttcgag gcactcgtcg tcaaaccggc
     7561 gaagcgtctc gaagactcga tcttctggat cgacgacggg ccggcgatcc gctacgcccc
     7621 ctcggccggc gtccagcggg cgctcgccga ccgggccccg gccggttcgg gcctcctgag
     7681 cgtcgccgac cccgcctaca ggaccctgcc gcggctgccc gggaccgagg cggagagccg
     7741 atcggtcgtc caggcgagcc ggcggaagga gctcggggcc tcccccgcgg tggtcctgac
     7801 cggagcggcc gcgaccgagt ccgcggtccg cgccgccctg ccgggaaaga agttcctcca
     7861 cctcgcggtg cacgggaagg tcgagccgcg cctcggcgac ctcttcgcgt ccctcgcgct
     7921 cgcggacgac gaagcgctga tgctcttcga gatctacgac ctcgacctgc aggccgagct
     7981 ggcggtcctg tcggcctgcg agacgagccg ggggcggatg gtggaagggg agggggcgtt
     8041 cgcgctgtcg cgcggcttcc tcgccgccgg ggcgcgccgg gtcgtcgcga gcctctggtc
     8101 ggtggacgac gcctcgaccg cggagctgat gaggaccttc ttccggcgcc tcggaccggg
     8161 ggacgaccgc tcctacgccg cggcgctgcg ggacgcgcgg cgggtgctgc gcgggaaagg
     8221 gaaatggaag tccccattct actgggcgcc gttcctcctc accggcgcct attgagccgc
     8281 gggagcccgg aggaggctgc ggtcgacgcg ggcggccgcg ccaggcgttt caggggcagt
     8341 tgcggccgct gatcggggcg ccgctgctgg tgtggccggg caggccggcg ccgcagaccc
     8401 cctccgcccg aatcaggaag tagtagcacc ctccgaccgg tgcgctgccg gtcatcgtgc
     8461 tcgtcaggtc ggcgccgttg ctctcgacgc agacggcggc gcccgaggcg aaatcgagcg
     8521 ggctcccgga tcgcagcgtg tcgtagaccg gcgcagtggt gctgccgaag tcgagcggcg
     8581 tcgtccaggt cagggtcgtg acgccggtcc cggtgttgtg ggccagcatg agactgcgcg
     8641 gctcggacgg ggggctccag cgcgaggagg cggcatcgtt gcagtccccc tggcactccg
     8701 agaacccgtc gaagtcgtcg tccccctcga tggtgccgac ggtggcatcg ggccagaagg
     8761 agacggagca gttgttgatc tgcccgtcgc aaatctccgg cgcgccgggg cgggtggcgg
     8821 cgcggttgtc gttgcagtcg ttgacgcagc cgtcgaaccc gtcgccgtcg cgatcgccgc
     8881 acggcgccgt gttcacgacg aagccgaggg cggtgaccgg ctcgaagccg gtccagaact
     8941 gcgcgtagta gaagtaatgg tcgttgtcga tcacccggcc gcggatgacg tcggtcgtga
     9001 aggtgcgcac cccaccggcg gcgccgctgc tggtcagggt cgccatcagc gtgttccccg
     9061 cccccgagct cgtgctgtcg gtgcggtaca gggacgcggt catgtcgccg tcgtcggagt
     9121 tgtccaggat cgacatcgac accgacagca cctgcgcgcc gtcgggcagc tgcaggccgg
     9181 tgttgaagcg gtactggccg gcgagcgtcc cctggaacgg caggcgcgag tcgctcgtct
     9241 gctccggcag gaacggcacc gccgcgacgg cgtcggagac ggtcgcagac ggcgaggccg
     9301 gcggcgtgta ggtgatccgc acgccgtaga tgtcgagggt cggatcgagg
//
LOCUS       DASRIQ010000646         8717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2601738, whole genome shotgun sequence.
ACCESSION   DASRIQ010000646 DASRIQ010000000
VERSION     DASRIQ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601738"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFD06_16040"
     CDS             complement(<1..597)
                     /locus_tag="VFD06_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05096.1"
                     /translation="MSGVSVPSPASAAPAAPAAVPSIRERLLGRTFVHPLFDYAIIGG
                     GLSLLMTAALVSAPKGDLLVTPALMSYLLLFCTSAHFAASTVRLYTKPGAAEGMPFLS
                     RWVPWVALALLAGILFAGGAAGRLVDTLYLAWSPYHYSAQAYGLAVVYSYRSGCRLAA
                     NDKKWLYWVAMLPFFFMLLHLAQTRLPDFTGIEAASVAA"
     gene            complement(612..977)
                     /locus_tag="VFD06_16045"
     CDS             complement(612..977)
                     /locus_tag="VFD06_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN05097.1"
                     /translation="MTVAPADPILDVIKRMRDRKVGSALVVEEDRLVGVFTERHIVTR
                     LTGLRAGEVEGMPVSKAMSRQPKSLREDDTLAFVVHRMAIDNVRHVPIEHPGRPLRFI
                     SVRRVLQFLDERARLAAAE"
     gene            complement(1159..2190)
                     /locus_tag="VFD06_16050"
     CDS             complement(1159..2190)
                     /locus_tag="VFD06_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZN05098.1"
                     /translation="MSDTTNAKPRLTKADFSSDQTVRWCPGCGDYSILAQTQKMMPAL
                     EIPRENIVFVSGIGCSSRFPYYMNTYGFHSIHGRAPTVATGLKIMRPDLSVWVATGDG
                     DGLSIGGNHLLHAMRRNLDIKILLFNNRIYGLTKGQYSPTSEYGKKTKSSPFGTAEQP
                     INPLEVAIASECTFVARSLDTDSQHLPMILERAARHHGTAFVEIYQNCNIFNDGAFDA
                     FAAKEVRSDKLLVLEHGKPMIFGKERNLGIRLVGLEPEVVTIGKDGVREQDVLVHDEK
                     RREPTLAYLLSRMTYPEFPVPMGVFYTATRPCLEDALERQATQATASMGAGSLERLLK
                     GGATWTVKD"
     gene            complement(2187..4058)
                     /locus_tag="VFD06_16055"
     CDS             complement(2187..4058)
                     /locus_tag="VFD06_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZN05099.1"
                     /translation="MTTETQIEPQRSAVQELDEVVIRFAGDSGDGMQLTGSQFADTTA
                     LFGNDLATLPDYPAEIRAPAGTLAGVSGYQINFSSHDIRTPGDQPNVLVAMNPAALRT
                     NLDDLEDGGILILDSDAFTDGNLNKAGYKTNPLRDGSLGRYRTFEIPLTSLTLKAVSE
                     TGLGGRHAARCKNFFALGLVFWMYERPVDHTRSWIEKKFKTNEQVKKANTLALQAGYN
                     YADTVEVFTTHYRVKKAVLPEGTYKSMTGNEATANGFIAAALLSGRPLFYGSYPITPA
                     SDILHELARHKNYPVKTFQAEDEISAIGSAIGAAYGGALGLTGTSGPGVALKGEAIGL
                     AVMTELPLVIANIQRGGPSTGLPTKTEQADLFQAILGRNGECPVAVIAPATPAECFYM
                     AIEAFRIAVKFMTPVFFLSDGYLANGSEPFRIPDVSTLPRFDVEFCTRAEGFQPYARD
                     PETLARPWAIPGTPGLEHRIGGLEKEDITGNVSYDPRNHEKMVRLRAEKIARIADFIP
                     EAQVTGAPSGKVLVVGWGSTHGAITSGVEAAQRQGKAVSSLHLRHLNPFPKNLGDVLN
                     RFEKVLVAELNLGQLRMLLRARYLVDAEGLNKVQGKPFKVSEITAAIDRLLGANQKR"
     gene            complement(4101..4568)
                     /locus_tag="VFD06_16060"
     CDS             complement(4101..4568)
                     /locus_tag="VFD06_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN05100.1"
                     /translation="MTIEEALRRERLGTLRLDPPISVPSGASLSEALGAMREAGGAAL
                     LVQKEGRTIGIFTERDVLNKLFPGTVDDRLPIDRFMTPDPASLTLDATLGEAVRLMTE
                     HGYRNVPLVDREGKSAGLLSARDVVQYVAEHFPAEVANLPPGLRQRFATKEGA"
     gene            4682..4870
                     /locus_tag="VFD06_16065"
     CDS             4682..4870
                     /locus_tag="VFD06_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05101.1"
                     /translation="MARTEPKVRGAGGPAGRPPLEPEHPLPVRLALFQLALLIGIPVA
                     FLLALKPILRAYFPELGY"
     gene            4928..5404
                     /locus_tag="VFD06_16070"
     CDS             4928..5404
                     /locus_tag="VFD06_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05102.1"
                     /translation="MPESTIIEAPGPADAPRPDRVRLVAHFKDGQISKGFSRDFDPRR
                     DSFHLEKRRAGIIASLEVRLEDLKALFHVKTWGRKDRHLGRPLGFPEGDLNRPPEGSY
                     VKTVAEFFDGERICGFSRGDHQERNGFYLIPADPEDNNRRIYVLRSSLVNIQIFRD"
     gene            5409..6050
                     /locus_tag="VFD06_16075"
     CDS             5409..6050
                     /locus_tag="VFD06_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05103.1"
                     /translation="MQHARGRPAWLLLVLALCAVAPALTFPTPAAAAPNRAAAKKEKN
                     RGPLLTFQSESGGERTAEASDLRFAFFQTLYRHRRAPRSESPTGERIEVVQKRKECNC
                     IRLADYTKIKKQVIRQIDITYPEGGRVALVRIISRDGSIREYEATHLYGGDGLFPPRF
                     AATVDGEHREFPLVLPDTPGASWPEERVVRMLVYRSTTAPPPPKKQKAGSESP"
     gene            6104..>8717
                     /locus_tag="VFD06_16080"
     CDS             6104..>8717
                     /locus_tag="VFD06_16080"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05104.1"
                     /translation="MAWSRALGQVLGGALPSQAIVLGTYLFGLGAGAAWASRKAGETS
                     RPLRLYARLEILVAAWGFAAPVLARAAALLIEKAGPSFADGAPIASLRLVAAALVLLP
                     GTLAMGATYPIAVQAVPAGMGRRGAALLYGVNTLAGAGGALLASFGLLPLLGTRRTFF
                     AAALLNLAAGLAALAVERHLARLHADDTDLPASRAPADSPSPAPGGATVSGRQGAPAR
                     SWLAAAGFTCGVAGALMQFGWTRAMTLAFGSTIYALGLTLSACLVGLGVGPLVVAKVT
                     RTRPARDLAAFSAWLAGAAGLLLLPVLGALPALGMRLSQVFEQHPFRALALQFAAAAL
                     LLLVPALAQGAMLPLLIETSLASATRASDDRLPAARSAGRIYAASTWGTVAGFLLAGF
                     VVVPRLGARLTLAVAAAILLVLGSWLVTRRRLLRAPLFAAPLLMLLLPGWDAAAMSAG
                     GFLYGPLYRAAFGRTAGPAREALAQAAARRGRVVFEREDGDGLTTVREAAGGTLSLQI
                     NGKTEASSGGDMPTQLLAGHLPLLMRPGARDVLVIGLASGVSLGAVERHPVRSIRVIE
                     IARGVPQAARLFAGPNRNALDDPRTALVVDDARAWMLARDERYDVIVSQPSNPWVAGV
                     ANLFTAEFYRLARSRLRPGGLMCQWVQAYRILPEDLRSVVRSFLDVFPDATLWEESAG
                     GGDYFLLGGLGDAGIDLDALARAPSDAWSDLGDAAGRGPVGLLTRFVAGPRALERFAA
                     AVPPHTDDALTLEWRAPLALFRDRPRGGLPLLPVEREQVLEHVAASEVPRDPALVEAL
                     TRSLSARQARLRAAAGLRDADRLALREPALAAGLDLLLAGRSAEAAATLARAAAAAPG
                     SANAHYLLGFAYREA"
BASE COUNT         1149 a         3120 c         3051 g         1397 t
ORIGIN      
        1 cgccgccacc gacgccgcct cgatcccggt gaagtccggc agccgcgtct gcgccaggtg
       61 caggagcatg aagaagaacg gcagcatcgc gacccagtac agccacttct tgtcgttcgc
      121 ggcgagccgg cacccggagc ggtacgagta gacgaccgcc aggccgtacg cctgcgccga
      181 gtagtgatag ggcgaccagg ccaggtacag cgtgtcgacg agccggcccg cggcgcctcc
      241 cgcgaacagg atgccggcca aaagcgccag cgccacccac ggcacccagc gcgacaggaa
      301 cggcatcccc tccgccgccc ccggcttggt gtagagccgc acggtcgagg cggcgaagtg
      361 ggcgctcgtg cagaagagca gcaggtacga catcagcgcc ggggtgacca ggaggtcccc
      421 cttcggcgcc gacaccagcg ctgcggtcat cagcaggctg agcccgccgc cgatgatggc
      481 gtagtcgaag agcgggtgga cgaaggtgcg gccgaggagg cgctcccgga tcgacgggac
      541 ggcggcggga gccgccgggg cggcgctcgc gggactcggg acgctcactc cgctcacggg
      601 attcgggacg ctcactccgc cgcggccagg cgcgcccgct cgtcgaggaa ctgcagcacc
      661 cggcgcaccg agatgaagcg cagcgggcgt ccggggtgct cgatcggcac gtgccgcacg
      721 ttgtcgatcg ccatgcggtg gaccacgaac gccagggtgt cgtcctcgcg cagcgacttc
      781 ggctgccggc tcatcgcctt cgacaccggc atcccctcga cttcaccggc gcggaggccg
      841 gtgaggcgcg tgacgatgtg ccgctcggtg aagacgccga ccagccggtc ctcctcgacc
      901 acgagggcgc tccccacctt ccggtcgcgc atccgcttga tgacgtcgag gatcggatcg
      961 gccggcgcga ccgtcaatgc cagtgccggc tgcagctcgc cgatggtcgt ctcgaggagc
     1021 gtgcggcgca ggccgaccct cgcgcgcgcg tcccgctccg ccgccgtcag cggctggcgg
     1081 cactcttcgc agaggtcggc gccgtcgaga ttcggatggc cgcagtcggg gcaggtcagg
     1141 gcccggcctc ctttcgtttc agtccttcac cgtccaggtc gcgcccccct tgaggagccg
     1201 ctcgagggat ccggccccca tcgaggcggt cgcctgggtc gcctgacgct cgagcgcgtc
     1261 ctcgagacac ggacgcgtgg cggtgtagaa gacgcccatc ggcaccggga actccgggta
     1321 ggtcatgcgc gagaggagat acgcgagggt cggctcgcgc cgtttctcgt cgtgcaccag
     1381 gacgtcctgc tcccgcacgc cgtccttgcc gatggtcacg acctccggct ccagcccgac
     1441 gaggcggatc ccgaggttgc gctccttgcc gaagatcatc ggcttgccgt gctcgagcac
     1501 cagcagcttg tcggagcgga cctccttcgc agcgaaggcg tcgaaggcac cgtcgttgaa
     1561 gatgttgcag ttctggtaga tctcgacgaa cgccgtcccg tggtggcggg ccgcgcgttc
     1621 caggatcatc ggcaggtgct gcgagtcggt gtcgagcgac cgcgccacga aggtgcactc
     1681 cgacgcgatc gccacctcga gcgggttgat cggctgctcg gcggtgccga aggggctcga
     1741 cttggtcttc ttgccgtact cgctcgtcgg cgagtactgc cctttcgtca gaccgtagat
     1801 ccggttgttg aacagcagga tcttgatgtc gaggttgcgc cgcatggcgt gcagcaggtg
     1861 gttgccgccg atcgagaggc cgtcgccgtc cccggtcgcc acccacaccg agagatcggg
     1921 ccgcatgatc ttgaggccgg tggccaccgt cggggcgcgc ccgtggatgc tgtggaaccc
     1981 gtacgtgttc atgtagtacg ggaagcggct ggagcagccg atgccggaga cgaagacgat
     2041 gttctcccgc gggatctcga gcgccggcat catcttctgc gtctgcgcca ggatcgagta
     2101 gtcgccgcag ccggggcacc agcggaccgt ctggtcgctg gagaaatccg ccttggtcag
     2161 ccggggcttc gcgttcgtcg tgtcgctcat cttttttgat tcgctcccag cagccggtcg
     2221 atcgcggcgg tgatttcgga caccttgaac ggctttccct gcaccttgtt gagcccctcc
     2281 gcatccacga ggtagcgcgc cctcaggagc atccgcagct gcccgagatt cagctcggcc
     2341 accagcacct tctcgaaccg gttcaggacg tcgccgaggt tcttcgggaa cgggttcagg
     2401 tggcgcaggt gcagggagga gaccgccttc ccctgccgct gcgccgcctc cacacccgag
     2461 gtgatggccc cgtgcgtgct cccccagccg acgacgagca ccttcccgga cggtgcgccg
     2521 gtcacctgcg cctccgggat gaaatcggcg atccgggcga tcttctcggc ccgcagccgc
     2581 accatcttct cgtgattccg cggatcgtag gagacgttcc cggtgatgtc ctccttctcc
     2641 agaccgccga tgcggtgctc gaggccgggc gtgcccggga tcgcccacgg gcgcgccagc
     2701 gtctccggat cgcgggcgta cggctggaac ccctccgcgc gggtgcagaa ctcgacgtcg
     2761 aagcgcggca gcgtcgagac gtccgggatg cggaaaggct ccgagccgtt ggcgaggtac
     2821 ccgtccgaca ggaagaagac cggcgtcatg aacttcacgg cgatgcggaa cgcctcgatc
     2881 gccatgtaga agcactcggc cggcgtcgcc ggggcgatca ccgccaccgg gcactcgccg
     2941 ttccgcccga ggatcgcctg gaagagatcc gcctgctcgg tcttcgtcgg cagccccgtc
     3001 gagggaccgc cgcgctggat gttggcgatg accagcggca gctcggtcat gaccgcgagc
     3061 ccgatcgcct cccccttcag cgccacgccg ggaccgctcg tgccggtcag gccgagcgcc
     3121 ccgccgtagg ccgcaccgat ggcggatccg atggcgctga tctcgtcctc cgcctggaac
     3181 gtcttcaccg ggtagttctt gtggcgcgcc agctcgtgca ggatgtcgct ggccggcgtg
     3241 atcggatagg agccgtagaa gagcggccgg ccggagagca gcgccgcggc gatgaagccg
     3301 ttcgccgtcg cctcgttgcc ggtcatgctc ttgtaggtcc cctccggcag caccgccttc
     3361 ttgacccggt aatgcgtcgt gaagacctcg acggtgtcgg catagttgta gccggcctgc
     3421 agcgccaggg tgttcgcctt cttcacctgt tcgttcgtct tgaacttctt ctcgatccag
     3481 gagcgggtgt gatccaccgg ccgctcgtac atccagaaga cgaggccgag cgcgaagaag
     3541 ttcttgcagc gggccgcgtg ccggccgccg aggccggtct cggacaccgc cttgagggtc
     3601 agcgaggtga gcgggatctc gaaggtccgg taccgcccga ggctgccgtc ccggagcggg
     3661 ttcgtcttgt agccggcctt gttgaggttg ccgtcggtga aggcgtcgga gtcgaggatg
     3721 aggatgccgc cgtcctcgag gtcgtcgagg ttggtgcgga gcgccgccgg attcatcgcc
     3781 acgagcacgt tcggctgatc gccgggggtg cggatgtcgt gcgacgagaa gttgatctgg
     3841 tagccgctca ctcccgcgag cgtcccggcc ggcgcgcgga tctcggcggg atagtcgggg
     3901 agggtcgcga ggtcgttccc gaagagcgcc gtggtgtcgg cgaactgcga tccggtgagc
     3961 tgcatcccgt cgccggaatc gccggcgaac cggatgacga cctcgtccag ctcctggacc
     4021 gcggaacgtt gaggctcgat ctgcgtctct gtggtcaccc ttctcgctcc ttgccgcgcc
     4081 ggaggatcgg cgcgggactg tcaggcccct tccttggtgg cgaagcgctg cctcaggccc
     4141 ggcggcaggt tcgccacctc cgccgggaag tgctccgcca cgtactgcac cacgtcgcgc
     4201 gccgaaagca gcccggcgct ctttccctcg cggtccacca gcgggacgtt gcggtagccg
     4261 tgctcggtca tgaggcgcac cgcctcgccg agcgtggcgt cgagcgtcag gctggccggg
     4321 tcgggcgtca tgaaccggtc gatcggcagc cgatcgtcca ccgtgcccgg gaacagcttg
     4381 ttgaggacgt cccgctcggt gaagatgccg atcgtcctgc cctccttctg gaccaggagg
     4441 gcggcaccgc cggcctcgcg catcgccccg agcgcctcgc tcaggctcgc ccccgagggg
     4501 acgctgatcg gcggatccag gcgcagcgtg ccgagccgct cccggcggag ggcttcctcg
     4561 atcgtcatcc cctctccctg tctcccggaa cgcttgactg taccatcatc gggccccccg
     4621 gggggtcgcc gcggccgacc tgtgataaag tccgcggccg cacgatcttc gggaggcgcg
     4681 cgtggcacgg accgagccga aagtcagggg tgccggaggc ccggctgggc ggccgccgct
     4741 ggagccggaa cacccgctcc cggtccgcct cgcccttttc cagctcgccc tgctgatcgg
     4801 cataccggtg gccttcctgc tcgctctcaa gccgatcctg cgggcgtact tccccgaact
     4861 ggggtactga agaactgcgt tcccgccgtc ccccggcgcc gcggagcctt cgttggccga
     4921 gcctgcgttg cctgagtcca ccatcatcga ggccccagga cccgccgacg ctccgcgacc
     4981 ggatcgggtc cggctggtgg cccacttcaa ggacggccag atctccaagg ggttctcgcg
     5041 cgacttcgac cccaggcgcg attcttttca cctggagaag cggcgcgccg gcatcatcgc
     5101 atcgctggag gtccggctcg aggacctgaa ggccctcttc cacgtcaaga cgtgggggcg
     5161 gaaggaccgg cacctcgggc ggccgctcgg gttcccggag ggggacctga accgcccacc
     5221 ggaggggtcg tacgtcaaga cggtcgcgga gttcttcgac ggcgagcgga tctgcggctt
     5281 cagccgcggc gatcaccagg aaaggaacgg tttctatctc atccccgccg atcccgagga
     5341 caataaccgc cgcatctatg tcctccgatc cagcctggtc aacatccaga tcttccggga
     5401 ctgaggcgtt gcaacacgcg cgggggcgcc ccgcgtggct ccttctcgtc ctggcgcttt
     5461 gcgccgtggc tccggcgctg acctttccga cgccggccgc ggcggccccc aaccgagccg
     5521 cggcgaagaa agagaaaaac cgggggccgc tcctgacctt ccaatccgag tccggcgggg
     5581 aacggaccgc cgaagcctcc gacctgaggt tcgccttttt ccagacgctc taccgacacc
     5641 ggcgcgcgcc ccgttccgag agccccacgg gggagcggat cgaagtggtg cagaagcgca
     5701 aagagtgcaa ctgcatccgg ctcgcggact acaccaagat caagaagcag gtgatccggc
     5761 agatcgacat cacctatccg gaggggggac gggtggcgct cgtccggatc atcagtcggg
     5821 acgggtcgat ccgcgaatac gaagcgaccc acctttatgg aggggacggg ctcttccctc
     5881 cccgcttcgc cgccactgtc gacggcgagc accgtgagtt cccgctcgtc cttcccgaca
     5941 cgccgggggc ctcctggccc gaggagcgcg tggtgcggat gctcgtctac cgttcgacga
     6001 ccgcaccccc tcctccgaag aagcagaaag ccgggagcga gagcccctga ccctgctgct
     6061 gttcctgttc ggactgtccg gtgcggccgg tctggcgcac gaagtggcgt ggtcgagggc
     6121 cctgggccag gtgctcggcg gagccctccc atcgcaggcg atcgtcctcg ggacgtacct
     6181 gttcggcctc ggcgccgggg ccgcgtgggc cagccggaag gcgggggaaa cgtcgcgtcc
     6241 gctgcgcctc tacgcccggc tggagatcct ggtcgcggcc tgggggttcg ccgcccccgt
     6301 tctggcgcgc gccgcggccc tgctcatcga gaaagccgga ccgtccttcg ccgacggggc
     6361 gccgatcgcc agtctgcggc tcgtcgcggc ggcgctcgtg ctcctgccgg gaacgctcgc
     6421 gatgggagcg acctatccga tcgccgtgca ggccgttccg gccgggatgg gccgccgggg
     6481 cgcggcgctc ctctacggcg tcaacacgct cgcgggcgcg ggcggggcgc tgctcgcgtc
     6541 gttcgggctc ctgccgctcc tcgggacccg gcgcaccttc ttcgcggcgg cgctcctgaa
     6601 cctggcggcc ggccttgccg ccctcgccgt ggaaagacac ctggcgcgcc tgcatgcgga
     6661 cgacaccgat cttcccgcct cccgtgctcc ggcggactcc ccttctcccg ctccgggcgg
     6721 ggcgactgtg tccggccgtc agggggcgcc ggcccggtcc tggctcgcgg ccgccgggtt
     6781 cacctgcggg gtggcaggag ccctgatgca gttcggctgg acgcgcgcca tgaccctcgc
     6841 cttcggctcc acgatttacg cgctcggtct gaccctgtcg gcgtgcctcg tcggcctcgg
     6901 cgtcgggccg ctcgtcgttg ccaaagtgac gcggacgcgc ccggcgcgcg acctcgccgc
     6961 cttctcggcc tggctcgcgg gcgccgcggg gctgctgctc ctccccgtgc tgggggcgct
     7021 tccggccctg gggatgcgcc tcagccaggt gttcgagcag cacccgttcc gggccctggc
     7081 gctgcagttc gcggcggcgg ccctgctgct cctcgtcccc gcgctggcgc agggggccat
     7141 gctccccctc ctgatcgaga cgagcctcgc ctcagccacc cgcgcgtcgg acgatcgcct
     7201 tcccgccgcc aggagcgccg ggcgcatcta cgccgcctcg acctggggga cggtggccgg
     7261 gttcctgctg gcggggttcg tcgtcgtgcc gcggctcggc gcgcgtctga cgctcgcagt
     7321 cgcggcggcg atcctcctcg tcctcggatc gtggctcgtg acgcgccggc gcctgctccg
     7381 cgcgccgctg ttcgcggcgc ctctcctcat gctgctcctc ccgggctggg atgcggccgc
     7441 catgtcggcc ggagggtttc tctacgggcc gctgtaccgg gccgcgttcg gccggacggc
     7501 cggcccggcc cgtgaagctc tggcccaggc ggcggctcgc cgcggccggg tggtcttcga
     7561 gcgggaggat ggggacggtc tcacgaccgt gcgcgaagcg gcggggggca cgctgtcgct
     7621 gcagatcaac ggcaagacgg aggcctcgag cggcggcgac atgccgacgc agctcctcgc
     7681 cgggcacctg cctctcctga tgcgacccgg tgcgcgcgac gtcctggtga tcggcctcgc
     7741 cagcggcgtc agcctcggcg cggtcgagcg acatccggtg cggagcatcc gggtgatcga
     7801 gatcgcccgg ggcgtgccgc aggcggcgcg gctgttcgcc ggcccgaacc ggaacgccct
     7861 cgacgacccg cgcaccgccc tcgtggtgga cgacgcgcgc gcctggatgc tggcacgcga
     7921 cgaacggtac gacgtcatcg tctcgcagcc ctcgaacccg tgggtggccg gcgtcgcgaa
     7981 cctgttcacc gccgagttct atcgtctcgc ccgctcccgc ctgcgcccgg gcggcctgat
     8041 gtgccagtgg gtgcaggcgt accgcatcct gccggaggat ctccggagcg tcgtgcggtc
     8101 gtttctcgac gtctttccgg acgccacgct ctgggaggaa tcggccgggg gcggcgacta
     8161 cttcctgctc ggcggtctgg gcgatgcggg gatcgatctc gacgcgctcg cgcgcgcccc
     8221 gtcggacgcc tggtccgatc tcggcgatgc ggcgggccgg ggaccggtcg ggctcctgac
     8281 ccgcttcgtc gccggaccgc gggccctgga gcgcttcgca gcggcggtgc cgcctcacac
     8341 cgatgatgcc ctgacgctcg aatggcgcgc cccgctggcg ctcttccgcg atcgtccgag
     8401 gggcggactc ccgctcctgc cggtcgagcg ggaacaggtg ctcgagcacg tcgcggcttc
     8461 cgaggtcccg cgggatccgg cgctcgtcga agcgctcacc cggagcctct cggcccggca
     8521 ggcacggctg cgggccgcgg ccggtctgcg cgacgccgat cggctggcgc tgcgcgagcc
     8581 ggcgctcgcc gccgggctcg acctcctgct cgccgggcgg tccgccgaag cggccgcgac
     8641 gctggcgcgc gccgcggccg ccgcccccgg cagcgccaat gcgcactacc tgctcggctt
     8701 cgcctatcgc gaggcgg
//
LOCUS       DASRIQ010000647         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2603982, whole genome shotgun sequence.
ACCESSION   DASRIQ010000647 DASRIQ010000000
VERSION     DASRIQ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2603982"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2015)
                     /locus_tag="VFD06_16085"
     CDS             complement(<1..2015)
                     /locus_tag="VFD06_16085"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HZN05105.1"
                     /translation="MIQAAELLLQERTPRAVAVTRPRAEEVLEAAHVRDLVPPTLRRF
                     DSPHDITPRTHLLSNGRYTVMLTAAGSGFSRWGDLAVTRWREDATRDCWGTFVFLRDA
                     ETGAVWSAGYQPCGTEADQYDVVYSEDRVKIARQDASLAITLEVVVSHEDDAELRRLT
                     VTNYENRRRNIDFTSYAEVVLAPQAADEAHPAFSNLFVETEFVGSHETLLATRRPRSA
                     EEPRVWLAHLAALEGEPVGPPRWETDRARFLGRGRGIRTPVAVLDGGPLSNTAGTVLD
                     PIVSLRHRVAIPPGGTVRLVYTTLVAPSREQALEVAEKYRQPATFERASSLAWTQAQV
                     QLHHLRITQDEAHLFQRLANRLLYSDRTLRAGPRELAASRGGRGGLWAHGISGDLPIA
                     VVRIEREDEREIARQLLRAHEYWGLKGIAADLVILNAKGTSYAQDLQQAVEAMVRSSQ
                     SAIGHETHGGHGNVFVLREDLLPPADLAVLRSAARVLILANRGTLSEQVVRQPSAAAE
                     PPAPRRRPAREKPQPSGLPALDLQSWNGLGGFTPDGREYVTVLDRGRWTPAPWINVVA
                     NPAFGFQVSESGSGSTWSVNSRENKLTPWSNDPVSDPPGEAWYVRDEESGLLWGPTCL
                     PIRAAGAEPYVIRHGAGYSRFEHHAHGIALELLQFVPPRDPIKISRLA"
BASE COUNT          302 a          741 c          732 g          288 t
ORIGIN      
        1 gcgaggcggg agatcttgat cggatcgcgg ggcggcacga actgcagcag ctcgagcgcg
       61 atcccgtgcg cgtggtgctc gaagcggctg tatcccgcac cgtgccggat gacgtagggt
      121 tcggcccccg ctgcgcggat cggcaggcag gtcggccccc agagcagccc gctctcctcg
      181 tcgcgcacgt accacgcctc gcccggcggg tcgctgaccg gatcgttcga ccagggggtg
      241 agcttgttct cgcggctgtt caccgaccag gtggaacccg agcccgattc ggagacctgg
      301 aacccgaagg ccgggttcgc cacgacgttg atccaggggg cgggggtcca ccggccgcga
      361 tcgagcaccg tgacatactc gcgtccatcc ggcgtgaacc ctccgaggcc gttccaggac
      421 tgcagatcga gcgcggggag gcccgacggc tgcggtttct cccgcgccgg ccggcgacgc
      481 ggcgccggcg gctccgcggc cgccgaaggc tgccgcacca cctgctccga gagcgtcccg
      541 cggttcgcca ggatcagcac gcgcgccgcc gagcgcagga ccgccaggtc cgcgggaggc
      601 aggaggtcct cccggagcac gaagacgttc ccatgcccgc catgggtttc gtgcccgatg
      661 gcggactggc tcgaccgcac catcgcctcg accgcctgct ggagatcctg tgcataggag
      721 gtccccttcg cattcaggat caccagatcg gcggcgatcc ccttcaaccc ccagtactcg
      781 tgggcgcgca ggagctgccg cgcgatctcc cgctcgtcct cccgctcgat ccgcaccacc
      841 gcgatcggca ggtcccccga gatgccgtgc gcccagaggc ccccacggcc gccgcggctc
      901 gcggccagct cccggggtcc ggcgcgcagg gtgcgatcgc tgtagaggag acgattggcc
      961 agccgctgga agagatgcgc ctcgtcctgg gtgatccgga gatggtgcag ctggacctgg
     1021 gcctgggtcc acgcgagcga gctggcgcgc tcgaacgtcg ccggctggcg atacttctcc
     1081 gcgacctcga gggcctgctc ccgcgaaggc gccaccaggg tcgtgtagac gagtcggacc
     1141 gttccccccg gaggaatcgc cacccggtgg cggaggctga cgatcggatc cagcaccgtc
     1201 ccggccgtgt tcgagagggg cccgccgtcg aggaccgcga ccggcgtccg gatgccgcgg
     1261 ccacgtccca ggaagcgggc gcggtccgtc tcccagcgcg gcgggccgac cggctccccc
     1321 tccagcgcgg ccaggtgggc gagccagacg cgcggctctt ccgccgaacg cgggcgccgc
     1381 gtcgccagca gagtttcgtg ggagccgacg aactccgtct ccacgaacag attcgagaag
     1441 gcgggatggg cttcgtccgc cgcctgcggg gccagcacga cctcggcgta cgaagtgaag
     1501 tcgatgttgc gccgccggtt ctcgtagttg gtcacggtga gacgacgcag ctccgcgtcg
     1561 tcctcgtggg agacgacgac ctcgagggtg atcgccagcg acgcgtcctg ccgggcgatc
     1621 ttgacccgat cctccgaata gacgacgtcg tactggtccg cctcggtccc gcagggctgg
     1681 tagccggccg accagacagc accggtctcc gcgtcccgca ggaaaacgaa cgtgccccag
     1741 cagtcgcgcg tcgcgtcctc ccgccagcgg gtgacggcca ggtcgcccca ccggctgaac
     1801 cccgaccccg ccgccgtcag catgacggtg tagcggccgt tggagagcag gtgcgtgcgc
     1861 ggggtgatgt cgtgcggcga atcgaaacgt cgcagggtgg ggggaaccag atcgcgaacg
     1921 tgcgccgctt ccaggacctc ctccgcccgg ggccgggtca cggcgaccgc acgcggcgtc
     1981 cgctcctgga gcagcagctc cgccgcctgg atcatgggat gggaatggaa gcgcctcggc
     2041 gtcgcggcgt catggaccac att
//
LOCUS       DASRIQ010000648         4023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_503522, whole genome shotgun sequence.
ACCESSION   DASRIQ010000648 DASRIQ010000000
VERSION     DASRIQ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503522"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /gene="sthA"
                     /locus_tag="VFD06_16090"
     CDS             complement(<1..1311)
                     /gene="sthA"
                     /locus_tag="VFD06_16090"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HZN05106.1"
                     /translation="MLELDLLVIGTGPAGQRAAVQAAKLGKTVGIAEQREVVGGVCIN
                     TGTIPSKTFREAILYLTGYEQRNFYGSGYRLKESITMPELLHRVEHVVEREIQVIRAQ
                     MARNRVRLIPGAASFVGPHDLVVQGAAETVEVRAERVAIACGTVPAAPAGVQVDGTTV
                     IDSDGILGLKEIPRTLTVVGAGVIGVEYASMFAALGVEVTLVDKRDRLLDFVDAEIAE
                     ALSYTMRNMNCTFRLREEVASIAIEAPRRAVATLRSGKHIVSDLLLYAVGRIGATASL
                     NLQAAGLEADERGRVKVNECFETAVPHILAIGDVIGFPSLASTSMEQGRRAACYAYGV
                     SCSSLPELFPYGIYSIPEISMVGRNEAELTEAGVPYEVGVARYREIARGAILGDDSGL
                     LKILFHRETRALLGVHIIGTSATELVHIGQAAIAFGATIDYFVDA"
     gene            complement(1354..3819)
                     /locus_tag="VFD06_16095"
     CDS             complement(1354..3819)
                     /locus_tag="VFD06_16095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN05107.1"
                     /translation="MGDLAPEIRQAVRSLRRAPGFTAAAIATLGFGLGASVLVWGTLH
                     AVLFRSLPFRDPGAIAAIFLITPDNGRFPLSIPDLLDYQAESKSFEGMVGVAGQSLNL
                     TGQGDAERLQGVRATGNFFDLLGVRAQIGRTLRPEDDLSGARLAVLGDGLWRRRFGAD
                     PAVVGRTLLLNGEPHEVVGVLPRRFRFPGGSAELAVPLAAGRDPVRQDRRAVGILRTI
                     GRLRPGVTLAAARAEMNAIAARLRESYPDANATKIGVNVTPYLEEITGAVRPSLFLLG
                     GAVLCVLLIGCVNLSGLALVRASGRSRELAIRMALGATRGRLARLVLIEALLLAAAGG
                     LAGGVAALWGMQALGALPPRVLPRAHEVGPGAFTFLFGVGLAIAAGLLSGALPAFLAG
                     RTEPIAGLKGESARPGPSRLARWLAIAPLLRSRRLLVGVEVALAMLLLAGTGLLLRSF
                     VRLNDVDPGFEQRQVLVARLALPKGRYARPEPMVRFHDELLDRLRLLPGVEAAGMISI
                     APMSGPLGSADVRREDRPPADPNAGMAAHYRVVSPGYFATMRIPLLAGRDFEASDRVS
                     APAVGIVNATLARLLFPGEDPIGRTLVVDDAPAGPRRVTIVGVVRDVRHEGLDVPPIP
                     DLHLPAAQVIPNVVQWLANNQFWSIRTAGDPAGLARAVKETVRGLDPDVSTSGLKTQK
                     AIVDEAAGTERLSLCLVMAFAGAALLLAAMGIYGLVAFSVSQRRREIGIRIALGARGR
                     DVERLVAGEGVRLAGAGLLAGGIAALPLCRLLAGFLFGIAPHDPATFAAVATTLFGVA
                     LLAAWIPARSAARIPPNEALRAE"
BASE COUNT          625 a         1467 c         1423 g          508 t
ORIGIN      
        1 cgcgtcgacg aagtagtcga tggtcgcgcc gaaggcgatc gccgcctggc cgatgtgcac
       61 cagctccgtc gcgctcgtgc cgatgatgtg gacgccgagg agcgcccggg tctcccggtg
      121 gaagaggatc ttgaggaggc ccgagtcgtc tccgaggatg gcgccacggg cgatctcacg
      181 gtaacgcgcc acgccgacct cgtacgggac cccggcctcg gtcagctccg cctcgttgcg
      241 accgaccatg gagatctccg ggatggagta gatgccatac ggaaagagct ccggcagcga
      301 cgagcaggag actccgtagg catagcaggc ggcccgccgc ccctgctcca tcgacgtgga
      361 ggcgagcgac gggaagccga tgacgtcccc gatcgccagg atgtgcggca ccgcggtctc
      421 gaagcactcg ttgaccttga cgcgcccgcg ctcatccgcc tcgaggccgg cggcctggag
      481 gttgagcgag gcggtcgccc cgatccttcc gacggcgtag agaaggagat cggagacgat
      541 gtgcttcccg gagcgcaggg tggcgacggc gcgccgcggc gcctcgatcg cgatcgaggc
      601 gacctcctcg cgcagccgga aggtgcagtt catgttgcgc atcgtgtagg aaagcgcctc
      661 ggcgatctcg gcgtccacga aatcgagcag gcggtcgcgc ttgtcgacca gagtcacctc
      721 gaccccgagc gccgcgaaca tcgaggcgta ctcgacgccg atgacccccg caccgacgac
      781 cgtgagggtg cgcgggatct ccttgaggcc gaggatcccg tccgagtcga tgaccgtcgt
      841 cccgtccacc tggacgccgg ccggggctgc cggcaccgtg ccgcaggcga tcgccacgcg
      901 ctcggcgcgc acctcgacgg tctccgcggc tccctggacc acgagatcgt gcggcccgac
      961 gaacgacgcc gcgccgggga tcaggcggac gcggttgcgc gccatctgcg cgcggatcac
     1021 ctggatctcc cgctccacca cgtgctcgac ccggtgcagg agctccggca tggtgatcga
     1081 ctctttcagg cgatagccgg agccgtagaa attgcgctgc tcgtagccgg tcaggtagag
     1141 gatcgcctcg cggaaggtct tgctggggat ggtgccggtg ttgatgcaga cgccgccgac
     1201 gacctcgcgc tgctcggcga tgccgaccgt cttccccagc ttggccgcct gcacggcggc
     1261 ccgctgaccg gcggggccgg ttccgatgac cagcagatcg agttcgagca cgcgcgcact
     1321 ttactatgac ccgccgtgcg gcgccgctgc cggtcactcg gcgcgcagtg cttcgttcgg
     1381 cgggatgcgc gccgcggacc gcgccgggat ccaggccgcg agcagggcga ccccgaacag
     1441 cgtggtggcc acggcggcga aggtcgccgg atcgtgcggc gcgatgccga agaggaaccc
     1501 cgcgagcagc cggcacaggg ggagggccgc gatcccgccg gcgagcagtc ccgcccccgc
     1561 cagacgcacc ccttcgccgg cgacgagccg ctcgacatcc cttccgcggg ctccgagcgc
     1621 aatccggatg ccgatctcgc gccgccgctg gctcaccgag aacgccacga ggccgtagat
     1681 ccccatcgcc gcgagcagca gagccgcccc cgcgaacgcc atcacgaggc agaggctgag
     1741 gcgctcggtc cccgcggcct cgtcgacgat cgctttctgg gtcttcaatc cgctggtgga
     1801 gacgtccgga tcgagcccgc gcacggtctc cttcaccgct cgggccagcc cggccgggtc
     1861 gccggccgtc cggatcgacc agaactggtt gttcgccagc cattgcacga cgttcgggat
     1921 gacctgcgcc gccggcaggt ggaggtcggg gatcggcggg acgtcgagcc cctcgtgtcg
     1981 aacgtcgcgc accacgccga cgatggtcac gcggcgcggg ccggcgggcg cgtcgtcgac
     2041 gacgagcgtg cggccgatcg ggtcctcgcc cgggaacagg agccgcgcca gagtcgcatt
     2101 gacgatcccg accgccggcg ccgagacccg gtcgctggcc tcgaaatcgc ggccggcgag
     2161 aagcgggatg cgcatcgtgg cgaagtagcc gggactgacg acacggtagt gcgccgccat
     2221 cccggcgttc ggatcggccg gcgggcgatc ctccctccgc acgtcggcgc tcccgagcgg
     2281 tccgctcatc ggcgcgatgg agatcatccc ggccgcctcg actccgggga ggagtcgcag
     2341 tcgatcaaga agctcatcgt ggaacctcac catcggctcg ggacgcgcgt accggccctt
     2401 cggcagcgcc aggcgcgcca ccaggacctg ccgctgctcg aagccgggat cgacgtcgtt
     2461 caagcggacg aaggaacgga gcaggagccc ggtcccggcc agaagcagca tggccagcgc
     2521 cacctcgact cccaccagca gcctgcggga acgcagcagc ggagcgatcg ccagccatcg
     2581 tgccagacgc gatgggcccg gcctcgccga ctcccctttc aggccggcga tcggctccgt
     2641 ccgcccggcg aggaacgccg gcagggcgcc ggagagcaga cccgcggcga tcgccaggcc
     2701 gacgccgaac aggaaggtga aggcgccggg accgacctcg tgcgcccgcg gcaggacgcg
     2761 ggggggaagc gcgccgagcg cctgcatgcc ccagagcgcc gccactcctc ccgcgagccc
     2821 tcccgccgcg gccagaagga gcgcctcgat caggaccagg cgcgccagcc ggcctcgcgt
     2881 cgcgccgagc gccatgcgga tcgccagctc gcggcttcgc ccggacgcgc ggaccagcgc
     2941 cagcccggac aggttgacgc agccgatcag caggacgcac aggacggcgc cgccgagaag
     3001 gaacagcgac gggcggaccg ctcccgtgat ctcctcgaga tagggggtga cgttcacgcc
     3061 gatcttggtc gcattggcgt cggggtacga ctcgcggagc cttgccgcga tggcgttcat
     3121 ctccgcgcgc gccgcggcca gcgtgacgcc gggacgaagc cggccgatcg tgcgcaggat
     3181 gccgacggcg cgccggtcct ggcgcaccgg atcgcggccg gccgcgagcg ggaccgccag
     3241 ctccgccgac ccgcccggga agcggaagcg ccgtggcagg acaccgacca cctcgtgcgg
     3301 ctcgccgttg agcaggagtg tgcggccgac gacggccggg tcggcgccga accggcgccg
     3361 ccacaacccg tcgccgagca cggcgaggcg cgccccggag aggtcgtcct ccggccgcag
     3421 ggtgcggccg atctgcgcgc gcacgccgag caggtcgaag aaattgccgg tggcgcgcac
     3481 tccctgcagg cgctccgcgt ccccctgtcc cgtgaggttg aggctctgtc cggcgacccc
     3541 caccatcccc tcgaaggact tcgactccgc ctggtagtcg agcaggtccg gaatggacag
     3601 cgggaagcgt ccgttgtccg gggtgatcag gaagatcgcg gcgatcgccc cggggtcgcg
     3661 gaacggcagc gaccggaaca ggaccgcgtg cagcgtcccc cacaccagca cgctggcgcc
     3721 gaggccgaac ccgagggtgg cgatcgccgc cgcggtgaac cccggcgccc gccgcagcga
     3781 gcgcacggcc tgccggatct ccggcgccag gtcccccatg ccgccgcctc cagtccgggg
     3841 ggccgggcgt ccgcgcccac cccgggtcgt tcgaaacgcc ccgccccctc ggacgagcgg
     3901 caccgtagca ccgccggcat gcatcaatcc aattgattgt tttctcgttc atcatgaatt
     3961 cggctcatgt aggatcggcc cggaggaaag cccatggatc tcgaaatccg ccacctgcgc
     4021 ctg
//
LOCUS       DASRIQ010000649         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_2343386, whole genome shotgun sequence.
ACCESSION   DASRIQ010000649 DASRIQ010000000
VERSION     DASRIQ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343386"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="sppA"
                     /locus_tag="VFD06_16100"
     CDS             <1..729
                     /gene="sppA"
                     /locus_tag="VFD06_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZN05108.1"
                     /translation="IKAIVFRVDSPGGSYVASDIIWREVTLTKGKKPIVVSMGDVAGS
                     GGYFVAMAADRIVAQPGTITASIGVLAGKFVTKDFWEMLGLTSDAIQRGRHATFFSSE
                     QPFTAEERAIFKGWLERIYKDFVGKVAQGRGKSFDEIHAIAQGRIWTGEDALKLGLVD
                     ELGGLPTAVARAAELAHLKPSDKVHLVEVPAPKSFFQELFDRDDSTETSLQKLRMQIR
                     SFVEEGRLPIRDEVLAMPYVPRVR"
     gene            733..924
                     /locus_tag="VFD06_16105"
     CDS             733..924
                     /locus_tag="VFD06_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05109.1"
                     /translation="MGTVLFVLGVLLFFGVVAFFIALISIPILLVIGLAVAAIRFAFF
                     LLTLPFRLVGWMFGLAVGR"
     gene            complement(955..2571)
                     /locus_tag="VFD06_16110"
     CDS             complement(955..2571)
                     /locus_tag="VFD06_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05110.1"
                     /translation="MIPGLRSRPGARLRPCPPWLALSLVLAAGTGLAALSPARALEGG
                     SPGGARLRLLASGGVLFFWDGASTNLRPAPAGLASAPQGFRSVLPLDDARALLIEPAE
                     LPPGKQKEKKVREGQAVVLGLSETGAERGGPVVSFEGSPNAAAVSPDGRRAYVLAWRG
                     EPGAGGRGWLHAIDVDRGEVADSSLLSTHAFGIATDKAGGRLYLSLKDRIQTYGAEPV
                     RVSWQYRSPGLNGPIAVAPEADLLAVGRGAEVALFDAARLAATATEERHARRDDATMV
                     TTLPFVVTRLAWSEDGSLLAAIGVHGLVFLDPGNGALLWPPAPSIDFSGATDALVLKF
                     PASDHDVVLALAPGGAVSALRAPVPSAPVVPATARLEEPPAPMAVAAATAQETPRKAP
                     QDSPPPPAPQQAEPPKDDPAPDPKQAEPAPKPAEPPAAEAGAAPTTEPAPEQEQASGP
                     PRLRGRISGKGVRSIVLYGPNNILKEALRVEIQPDGTWNLELPAPGTYRVVAIGDGST
                     PIPVVPTFRTVTVRAGIGESGLDFEVRPAS"
     gene            2706..3977
                     /locus_tag="VFD06_16115"
     CDS             2706..3977
                     /locus_tag="VFD06_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05111.1"
                     /translation="MASLFDPVDDIKLPDDRPKRSYKGIIKIGGIVLGAIVLVGGGWY
                     TYDAYKPRKLTEKPLPASPDVLLSELKEARDAIDSNTRDIYARIGQFNDRMEMLGRKQ
                     INPSQVFLQGLSMEEQQAFDTLIKEEKDPSYRGVLAQVVENMKKIRELQEKVTEMEAK
                     LPGDGIEVKPGDTHMKMAQKYLMDTHKIPEARAKELASRINILETGLAKGNKVHFYYD
                     PTKDFFGTWVSQGDAKSSPLAVVRAREMGLIGERDKAVAKATDLEGKKAELEEVLANL
                     QAEIRSLEARKASLESNVAQLEGERNQAQEQAKTVSANLETVTNSMYYEADLADRLKA
                     RGVLKTVNKVDKIADVKFGSSLNLKQSKSITFKPTQFGIDRIRDVRILPSYFREKREL
                     DVKFADDGTVEVTVLDENAMRGQRVLFVVER"
     gene            3979..>4361
                     /locus_tag="VFD06_16120"
     CDS             3979..>4361
                     /locus_tag="VFD06_16120"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HZN05112.1"
                     /translation="MKPAGSPDPSDPVPAAAPAEAAFEDYPLTRQEYISAMVHFYRGE
                     MYRSQVWRTRLDTTTNWAVVTTAAVISFTFGDPNHPHVLLLLSNLIITVFLFHEARRF
                     RYFDVYRARVRMLEENFYLPMLTRQL"
BASE COUNT          733 a         1468 c         1537 g          623 t
ORIGIN      
        1 atcaaggcga tcgtcttccg cgtcgacagc ccgggcggct cgtacgtcgc ctccgacatc
       61 atctggcgcg aggtcaccct gacgaaggga aagaagccga ttgtcgtgtc gatgggggac
      121 gtcgccgggt cgggcgggta cttcgtcgcg atggcggcgg accggatcgt cgcgcagccg
      181 gggaccatca ccgcgtcgat cggcgtgctg gccggaaagt tcgtcaccaa ggacttctgg
      241 gagatgctcg ggctcacctc cgacgccatc cagcgcggtc ggcacgccac cttcttctcc
      301 tcggagcagc cgttcacggc ggaggagcgg gcgatcttca agggctggct cgagcggatc
      361 tacaaggact tcgtcggtaa ggtggcccag gggcgcggca agtccttcga cgagatccac
      421 gccatcgcgc aggggcgcat ctggaccggc gaggacgcgc tgaagctcgg gctcgtcgac
      481 gagctgggcg ggctgccgac ggccgtggcg cgcgccgccg agctggccca cctcaagcca
      541 tccgacaagg tgcacctcgt cgaggtcccg gcgccgaagt cgttcttcca ggagctgttc
      601 gacagggacg attcgaccga gacgagcctc cagaagctgc gcatgcaaat ccgctccttc
      661 gtcgaggagg ggcgcctgcc gatccgggac gaggtgctgg cgatgcccta cgtcccgcgg
      721 gtgaggtgaa cgatggggac ggtcctgttc gtgctcggcg tcctgctttt cttcggggtc
      781 gtggcgttct tcatcgcgct gatctcgatc ccgatcctgc tcgtgatcgg actcgccgtc
      841 gcggcgatcc gcttcgcgtt cttcctgctg acgctgccgt tccggctggt cggttggatg
      901 ttcgggctgg cggtcgggcg gtaggggagt cggtcaggga caagctctcc cggctcagga
      961 ggccgggcgg acctcgaagt cgaggccgga ttctccgatg ccggcccgca ccgtcaccgt
     1021 cctgaacgtc ggcacgaccg ggatcggcgt cgagccgtcg ccgatggcga cgacccggta
     1081 ggtcccgggg gccggcagtt cgagattcca ggtgccgtcc ggctgaatct ctacgcgcaa
     1141 cgcttccttc aggatgttgt tcgggccgta gaggacgatc gaccgcaccc ctttgcccga
     1201 gatgcgtccg cggaggcgcg gcggcccgct ggcctgctcc tgctccggcg cgggctcggt
     1261 cgtcggcgcc gccccggcct cggccgccgg cggctcggcc ggcttcggcg ccggctcggc
     1321 ctgtttggga tcaggcgccg ggtcatcctt cggcggctca gcctgttgcg gggccggcgg
     1381 gggtggggaa tcctgggggg ccttccgggg cgtttcctgg gccgtagcgg cagccaccgc
     1441 catcggggcc ggtggctcct ccaggcgggc tgtcgcaggg acgaccgggg ccgaagggac
     1501 aggggcgcgc agggccgaca cagcgccgcc cggggcgagc gccagcacga cgtcgtggtc
     1561 cgacgcgggg aacttcagga ccagcgcgtc ggtcgcgccg gagaaatcga tcgacggagc
     1621 cgggggccac aggagcgcgc cgttccccgg atcgaggaag acgagaccat gcacgccgat
     1681 cgccgccagg agcgacccat cctccgacca ggccagccgg gtcacgacga agggaagggt
     1741 cgtcaccatg gtggcgtcgt ccctgcgggc gtggcgctcc tcggtggcgg tcgccgccag
     1801 gcgggccgcg tcgaacaacg ccacctcggc gcccctcccg accgcaagca ggtcggcctc
     1861 cggcgccacg gcgatggggc cgttcaggcc gggagagcgg tactgccacg acacccggac
     1921 cggctccgct ccataggtct ggatccgatc cttgaggctc aggtagaggc gtccgccggc
     1981 cttgtcggtg gcgatgccga aggcgtgcgt ggagaggagg ctcgaatcgg ccacctcccc
     2041 gcgatcgacg tcgatggcgt gcagccagcc gcggcctccc gctcccggct cgccgcgcca
     2101 ggccagcacg taggcccggc gcccgtcggg agaaaccgcc gcggcgttcg gagacccttc
     2161 gaagctcacc actggtccgc cgcgctccgc accggtctcg ctcagcccca ggaccaccgc
     2221 ctgcccttcg cggaccttct tctccttctg cttgccggga ggaagctcgg ccggctcgat
     2281 cagcagcgcg cgcgcgtcgt cgagcggcag gaccgaacgg aacccctggg gagcggaggc
     2341 gagcccggcc ggagcggggc gcaggttggt cgaagccccg tcccagaaaa acagcacgcc
     2401 gccgctcgca aggagccgca gccgggcccc tcccgggctg cccccctcga gagcgcgggc
     2461 cggcgagagg gccgcgaggc cggtccccgc tgccagcacg agcgacaggg ccagccacgg
     2521 cggacagggg cgtaaccgcg cgccgggccg ggaccggagt ccagggatca tcggaacgac
     2581 aagtgtatcg cgacgcgcgg gctgccgccc gcagacccgc caaaggtccg gcaacagggc
     2641 cgttgcgccg ggctcgggcc gcgggctaaa atgcgccacc gcacaccaag ggagaggact
     2701 caagaatggc gagcctgttc gaccctgtcg atgacatcaa actgccggac gatcggccga
     2761 agagaagcta caagggaatc atcaagatcg gcggcatcgt cctcggggcg atcgtgctcg
     2821 tcggaggcgg ctggtacacc tacgatgcgt acaagccgcg caagctgacc gaaaagccgc
     2881 ttcctgcctc cccggacgtg ctgctcagcg agctgaagga ggcgcgcgac gccatcgaca
     2941 gcaacacccg cgacatctac gcccgcatcg gccagttcaa cgaccgcatg gagatgctgg
     3001 ggcgcaagca gatcaacccg tcgcaggtct tcctccaggg gctgtcgatg gaggagcagc
     3061 aggcgttcga caccctgatc aaggaggaga aggacccctc ctaccgcggc gtcctggccc
     3121 aggtcgtcga gaacatgaag aagatccggg agctgcagga gaaggtgacc gagatggagg
     3181 cgaagctccc cggcgacggc atcgaggtga agccgggcga cacgcacatg aagatggcgc
     3241 agaaatacct gatggacacc cacaagatcc cggaggcgcg cgccaaggag ctggcgtcgc
     3301 gcatcaatat cctcgagacc gggctggcga aggggaacaa ggtccacttc tactacgacc
     3361 cgaccaagga cttcttcggc acgtgggtgt cgcaggggga cgccaagtcg tcgccgctcg
     3421 ccgtggtgcg ggcgcgcgag atggggctca tcggcgagcg cgacaaggcg gtcgccaagg
     3481 cgaccgacct cgaggggaag aaagcggagc tcgaggaggt gctggcgaac ctgcaggcgg
     3541 agatcaggtc gctggaggcc cgcaaggcat cgctcgagtc gaacgtcgcg cagctcgaag
     3601 gggagcggaa ccaggcgcag gagcaggcga agaccgtcag cgccaacctc gagacggtga
     3661 cgaactcgat gtactacgag gcggacctcg ccgaccggct gaaagcgcgc ggcgtcctca
     3721 agacggtcaa caaggtggac aagatcgccg acgtgaagtt cggctccagc ctgaacctca
     3781 agcagtcgaa gagcatcacc ttcaagccga cccagttcgg catcgaccgc atccgcgacg
     3841 tgcgtatcct cccttcgtac ttccgggaga aacgggagct ggacgtgaag ttcgcggacg
     3901 acggaacggt cgaggtgacc gtcctcgacg agaacgccat gcgcggccag agggtgctct
     3961 tcgtcgtcga gcggtagcat gaagccggcc ggttcgccgg atccgtccga ccccgtgccg
     4021 gccgccgccc ccgcggaagc ggccttcgag gactatcccc tcacgcgtca ggagtacatc
     4081 agcgccatgg tgcacttcta ccgtggtgag atgtaccgca gccaggtctg gcgcacgcgg
     4141 ctcgacacca ccaccaactg ggcggtcgtg acgacggcgg cggtcatctc cttcacgttc
     4201 ggcgacccga accacccgca cgtcctgctc ctcctctcga acctgatcat cacggtcttc
     4261 ctgttccacg aggcccggcg gttccgctac ttcgacgtct accgcgcgcg ggtgcggatg
     4321 ctggaggaga acttctacct gccgatgctg acgcggcagc t
//
LOCUS       DASRIQ010000650         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_505250, whole genome shotgun sequence.
ACCESSION   DASRIQ010000650 DASRIQ010000000
VERSION     DASRIQ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505250"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1928
                     /locus_tag="VFD06_16125"
     CDS             <1..1928
                     /locus_tag="VFD06_16125"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN05113.1"
                     /translation="TSILRDILMLGGDGERLVLRSTTAMPGTSNDCGVAVGRLDADAH
                     LDLVVGASGAGLATFRGDGAGGFAFNGSVAAGSGLEYPMLGDFDEDGDADIVASNTSG
                     VFFLRGHGDGSFEPEVQVAAISSNLRIAAARLDADAHLDLAVGAYQAGTVQVFAGTGV
                     GTFAPGASVAVGSSPQVVAAGDFDGDGNNDLAALVTLSGPGNLAVMFGAADGSLTAPL
                     TIPLDTSSSRMTTADFDLDGRDDIAVAYFVGHEAIFFGEPGRTFTQLRFAGATANGIA
                     SDNFEAADFNRDGRPDLISGSLTGVALLLNRGIPGSDSPIPPEAAITGAASFECDAPG
                     TGTISLDGLASTGTDLVSYEWLLDTPSGLEPLGTGLTLSVALPLGPATIVLRVTNALG
                     GSDDDVADVLVVDSIPPTFSAALAPGSPALLWPPNHRLVTIATQWNVSDVCDPAPAVA
                     LFAVTSDEPDDAPGGADGMTLVDIRDAETGTADASVQLRAERSSSGDGREYRLAYFAS
                     DASGNSAAADVVLSVPLHAFGEDDPLSLDVTPVGLGSGVVLSWTPVGSAYDVITGFLG
                     GFTVVDSELRLGNVEVLAAGTNDTSVLDDPPRLQPASGQAVFYLVQYHDAFGPTGYGS
                     EPVPWPRVPTACSGGCP"
     gene            complement(1957..>2617)
                     /locus_tag="VFD06_16130"
     CDS             complement(1957..>2617)
                     /locus_tag="VFD06_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05114.1"
                     /translation="EGTCIALPFGGGRLVCIPQQLIPPSVSACSGKAASDLCSFTRDG
                     RTIDGNCVAVPGHDLLVCLPPPPQGLIDACVGKAAGDSCSASLGDHEFTGACATAPDG
                     VTLACLPRRHDDDDDDESPREAACEGLPQGALCTVTVDDRTKNGTCQRDDDDDDRLAC
                     VPPAPPQEAIDACTGLMDGDTCSFTFRDHTVSGTCRALPGGATLVCAPLCPHPRKDRD
                     D"
BASE COUNT          339 a          958 c          909 g          411 t
ORIGIN      
        1 tcacctcgat cctgcgcgac atcctgatgc tcggcggaga cggcgaacgg ctcgtcctgc
       61 gatcgacgac cgcgatgccg gggacgtcta acgactgtgg ggtcgccgtc ggccggctcg
      121 acgccgatgc ccacctggat ctcgtcgttg gcgcctcggg ggcgggtctg gccacgttcc
      181 gtggcgacgg cgccggcggc ttcgctttca acggatcggt cgccgccggc tccggcctgg
      241 agtatccgat gctcggcgac ttcgacgagg acggcgatgc cgacatcgtc gcgtccaaca
      301 ccagcggggt cttcttcctg agaggacacg gggacggctc gttcgaaccg gaggtgcagg
      361 tcgcggcgat ttcctcgaac ctcaggatcg ccgccgcccg gctcgacgcg gacgcgcacc
      421 tcgatctggc ggtcggggcc taccaggcgg ggacggtgca ggtctttgcc ggcaccggcg
      481 tcggcacctt cgcgccgggc gcgagcgtcg cggtcgggtc atcgccgcag gtggttgcgg
      541 ccggcgattt cgacggcgat ggcaacaacg acctggccgc cctggtcacg ctctccggcc
      601 cgggaaatct cgcggtgatg ttcggcgccg ccgacggctc gctcaccgcg ccgctgacga
      661 tcccgctcga cacctccagc tcgcgcatga ccaccgccga tttcgacctc gatggacgcg
      721 acgacatcgc cgtggcctac tttgttggac acgaggcgat cttcttcggc gagccgggtc
      781 gcaccttcac gcagctgcgc ttcgccggcg ccactgcgaa cggcattgcc tccgacaact
      841 tcgaggccgc ggacttcaac cgggacgggc gacccgacct catctccggc tcgctcaccg
      901 gggtggcgct gctcctgaat cggggcatcc cgggatccga ctctcccatt ccgcccgagg
      961 cggcgatcac cggcgccgcg tccttcgagt gcgacgcgcc cggaaccggg acgatcagcc
     1021 tcgacggcct ggcctccacc ggcaccgatc tcgtttcgta cgagtggctc ctcgacaccc
     1081 cctcgggtct cgagccgctg gggacgggtc tgacgctatc ggtcgccctc ccgctcgggc
     1141 cggccacgat cgtcctgcgc gtcaccaacg cgctgggcgg gagcgacgac gacgtcgcgg
     1201 acgtgctggt cgtggactcg atcccgccca ccttcagcgc cgctcttgct ccgggttcgc
     1261 cggcgctcct ctggccgccg aaccaccggt tggtgacgat cgcgacgcag tggaacgtct
     1321 cggatgtctg cgaccccgcc cccgcggtgg ctttgttcgc cgtcaccagc gacgagccgg
     1381 acgacgcccc gggcggcgcg gacggcatga cgctggtgga catccgcgac gcggagaccg
     1441 gcaccgccga cgcgagcgtg caactgcgcg ccgagcgctc ctcgtcgggg gacggccgtg
     1501 agtaccggct cgcatacttc gcctcggacg cctccgggaa ttcggccgcc gcggacgtcg
     1561 tcctgtccgt gccgctgcac gccttcggcg aggacgatcc cctgtcgctc gacgtcactc
     1621 ccgtcgggct cggcagcggg gtggtcctct cgtggacccc ggtggggtcc gcctacgacg
     1681 tcatcaccgg attcctcggc gggttcaccg tcgtggacag cgagctgcgg ctcgggaacg
     1741 tcgaggtgct cgccgccggg acgaacgaca cctcggtcct cgacgacccg ccgcgcctgc
     1801 agcctgcgtc gggccaggcg gtcttctacc tcgtccagta ccacgacgcg ttcggcccga
     1861 ccggctacgg cagcgagccg gtcccctggc cgcgcgtccc gactgcctgc tcgggcgggt
     1921 gcccgtagag agcgacgcga tgttgcgcgt gcgggatcaa tcgtcccgat ctttccgcgg
     1981 atgcggacag agcggggcgc agacgagcgt cgcgccgccc ggcagggcgc ggcaggtgcc
     2041 gctcaccgtg tggtccctga aggtgaacga gcacgtgtcc ccgtccatca ggccggtgca
     2101 cgcgtcgatc gcctcctgag gcggcgccgg cggcacgcac gccagccggt cgtcgtcatc
     2161 gtcgtccctc tggcaggtgc cgttcttcgt ccggtcatcg acggtcaccg tgcagagcgc
     2221 cccctggggg agaccttcgc aggcggcctc gcgcggtgac tcgtcatcgt catcgtcgtc
     2281 gtgtcgccgc ggcaggcagg cgagcgtgac gccgtcgggc gccgtggcgc aggcgccggt
     2341 gaactcgtga tcccccaggg atgccgagca cgagtctccc gccgccttgc cgacgcaggc
     2401 gtcgatcaac ccctgcgggg gcggcggcag gcagaccagc aggtcgtgcc cggggacggc
     2461 gacgcagttc ccgtcgatgg ttcggccgtc gcgggtgaag gagcagaggt cgctggcggc
     2521 cttgccgctg caagccgaga ccgacggcgg aatcaactgc tgcgggatgc agaccagccg
     2581 ccctcccccg aacggcagtg cgatgcaggt cccctcg
//
LOCUS       DASRIQ010000651         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_3380590, whole genome shotgun sequence.
ACCESSION   DASRIQ010000651 DASRIQ010000000
VERSION     DASRIQ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3380590"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2850)
                     /locus_tag="VFD06_16135"
     CDS             complement(<1..2850)
                     /locus_tag="VFD06_16135"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN05115.1"
                     /translation="MTLSDLSIKNHVFAWMLMAALILFGAIGYARLGVSQNPDIDFPV
                     INVSVTLEGAAPEVMETDVIEPLEDAVVAVEGIKGISSTARQGRASIVVEFDLKRDID
                     LALQDVQTRVAQSARLLPREIDPPVITKTNPEDQPILWLNLSGDRPPGALADYIRNVL
                     KPRFQTLPGVGEITLGGYLERNIRVWLDARRLEQFSLTADDVTRAIQAQHVEVPAGRI
                     ETGDREMNVRAEGEAIDVAAFSALVVATKDGAPVHLSDVAVVEDGLEDRRRMARAVGL
                     PALGMGIRKQRGANAVEVAHLVKDRLREVQATLPEGMILAVNFDGTEVVEEAIHEIQF
                     SLLLAALLTGVVCWLFLGSWSSTVNVLLAIPTSVIGTFLCMYFFGFTLNTFTVLGLSL
                     SVGIVVDDAIMVLENIYRHRELGQSRLEAALVGAREITFAAMAATAAIVAVFLPVAFM
                     SGIIGRFFFEFGVTISVAVLLSLLEALTLTPMRCSRFLETGARAGGFGARIDRLFARL
                     ASGYRSWLGPALRHRGWVLTGAAAVFALSLGVLFVINREFVPSQDLSRFMVRMQTPVG
                     SSIDYTDRLTRQAEAFLMSRPEVYRYFAVVGGFGGGGGGEVNNSMAFVTLKPKGERPV
                     DPQAGRRLSTQAFMDVARKELGAIPGLKVTMQDLSQQGFSAQRGFPVELSVRGPDWDQ
                     LARHATTLMERMRATGQVTDVDTDYQVGMPEVKVIPDRRRAADLGISMASIGSTVNTL
                     IGGARVARFKDGGRRYDIRVRLQADQRQNPDDIRRLLLRTPSGGLVRLGDIVQIVERP
                     TLQVITHRDRQRAIGVFANLAAGASQATVLKDIERLGREVLPDGYTLAFSGSAQAFQE
                     SFRSLLFALGMGLIVAYMILASQFNAFSHPITVLLALPFSASGALFALYLGGQSLNIY
                     SMIGLILLMGIAKKNSIILVDYTNQMRRQGLDRDAA"
     gene            complement(2847..>3485)
                     /locus_tag="VFD06_16140"
     CDS             complement(2847..>3485)
                     /locus_tag="VFD06_16140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN05116.1"
                     /translation="PGIINRKSVVTGQHVDSDVVLATLVDTRRLRLRFRVTESESIRL
                     KDGLPVTFTTAAWPGRTFEARLYHVSRSADPGSRMVECLALLDDREAVLKPGFFAEVR
                     AATERRAGALLVPERAVLATENGFVVFEAEGNVARRREVTVGLRAGDGAMEILNGLKP
                     GAVVVTDGNASLRDGAAIEPVDSPTAPRSAGVPPAPPAPGDPGDASSAAPAR"
BASE COUNT          548 a         1197 c         1255 g          485 t
ORIGIN      
        1 cgccgcgtcc cggtcgagcc cctgacgccg catctggttg gtgtagtcca ccaggatgat
       61 ggagttcttc ttggcgatcc ccatgaggag gatgaggccg atcatcgagt agatgttgag
      121 cgactgcccg ccgaggtaca gcgcgaacag cgcccccgag gcggagaacg gcagcgccag
      181 caggaccgtg atcgggtggc tgaaggcgtt gaactgcgac gccaggatca tgtaggcgac
      241 gatgagcccc atgccgaggg cgaacagcag cgaccggaac gattcctgga acgcctgcgc
      301 cgagccggag aaggcgagcg tatagccgtc cggcaggacc tcgcgcccca gccgctcgat
      361 gtccttgagg accgtggcct gcgacgcccc cgccgcgagg ttggcgaaca cgccgatcgc
      421 ccgctgccgg tcgcggtggg tgatcacctg gagggtcgga cgctcgacga tctgcacgat
      481 gtccccgagg cgcacgagcc ccccggacgg cgtgcgcagg aggagccggc ggatgtcgtc
      541 cgggttctgc cgctggtcgg cctgcaggcg cacacggatg tcgtagcgcc ggccgccgtc
      601 cttgaagcgc gccacccgcg cgcccccgat cagagtgttc acggtcgatc cgatcgacgc
      661 catgctgatg ccgagatcgg cagcgcggcg gcgatccggg atcaccttca cctccggcat
      721 gccgacctgg tagtcggtgt cgacgtccgt cacctgcccg gtggcgcgca tcctctccat
      781 gagggtcgtg gcgtgccgcg ccagctggtc ccagtcggga ccgcgcaccg acagctccac
      841 cgggaagccg cgctgcgcgc tgaatccctg ctgcgacaga tcctgcatcg tcaccttgag
      901 ccccgggatg gcgcccagct ctttgcgggc cacgtccatg aacgcctggg tcgacaggcg
      961 tcgcccggcc tgcgggtcga ccggccgttc ccccttgggc ttgagggtga cgaaggccat
     1021 cgagttgttg acctcgccgc cccccccgcc gccgaacccg ccgacgaccg cgaaatagcg
     1081 gtacacctcg gggcgcgaca tcaggaacgc ctccgcctgg cgggtgaggc ggtcggtgta
     1141 gtcgatcgac gatccgaccg gcgtctgcat ccgcaccatg aagcgggaca ggtcctgcga
     1201 cggcacgaac tcgcggttga tcacgaagag cacgccgagc gatagggcga acaccgcggc
     1261 cgccccggtc aggacccagc cgcggtggcg cagggccggg ccgagccagc tccggtagcc
     1321 ggacgccagc cgcgcgaaga gtcgatcgat ccgcgccccg aagccgccgg cccgcgcgcc
     1381 ggtttcgagg aagcgcgagc agcgcatcgg ggtcagcgtc agcgcctcga gcagcgacag
     1441 caggaccgcg accgagatgg tgacgccgaa ttcgaagaag aaccgcccga tgatccccga
     1501 catgaacgcg accggcagga agacggcgac gatggccgcg gtcgccgcca tggcggcgaa
     1561 ggtgatctcg cgcgccccca ccagggccgc ctcgaggcgc gactgtccca gctcgcggtg
     1621 ccggtagatg ttctccagca ccatgatggc gtcgtccacg acgatgccga ccgacagcga
     1681 cagccccagc accgtgaagg tgttgagggt gaagccgaag aaatacatgc acaggaaggt
     1741 cccgatgacc gacgtcggga tcgccaggag cacgttgacc gtcgacgacc atgagccgag
     1801 gaacagccag cagacgacac cggtgagtaa tgccgcgagc aggagggaga actggatctc
     1861 gtggatcgcc tcctccacca cctcggtgcc gtcgaagttg accgccagga tcatcccctc
     1921 ggggagggtc gcctgcacct cgcgcaggcg gtccttgacg aggtgcgcca cctccaccgc
     1981 gttggcgccg cgctgcttcc tgattcccat cccgagcgcc ggcaggccga cggcgcgcgc
     2041 catgcggcgg cggtcctcca ggccgtcctc gaccaccgcc acgtcggaca ggtgcaccgg
     2101 ggcgccgtct ttggtcgcga cgacgagagc ggagaaggcg gccacgtcga tcgcctcccc
     2161 ctcggcgcgc acgttcatct cccggtcgcc ggtctcgatc cgccccgccg ggacctcgac
     2221 gtgctgcgcc tggatggcgc gggtgacgtc gtcggccgtg agcgagaact gctccagccg
     2281 acgcgcgtcg agccagacgc ggatgttgcg ctcgaggtag cctccgaggg tgatctcgcc
     2341 gacgccgggg agcgtctgga accggggctt gaggacgttg cggatgtagt cggcgagcgc
     2401 gccagggggc cggtcccccg acaggttgag ccagaggatc ggctggtcct cggggttggt
     2461 cttggtgatc accggcgggt cgatttcgcg cggcagcagg cgcgccgact gggcgacgcg
     2521 cgtctgcacg tcctgcagcg ccaggtcgat gtcgcgcttg agatcgaact cgacgacgat
     2581 gctggcccgg ccctggcggg cggtcgagga gatccccttg atcccctcca ccgcgacgac
     2641 ggcgtcctcg agcggctcga tgacgtcggt ctccatcacc tccggggcgg ccccctcgag
     2701 cgtgacggag acgttgatca ccgggaagtc gatgtccggg ttctggctga cgccgagccg
     2761 ggcgtagccg atggcgccga acaggatgag ggcggccatc agcatccacg cgaagacgtg
     2821 gttcttgatc gagaggtcgg agagcgtcat cgtgcgggtg cggccgacga ggcgtcgccc
     2881 gggtcgccgg gcgccggcgg cgcgggcggg acgccggccg agcgcggcgc ggtgggggag
     2941 tcgaccggct cgatcgcggc gccgtcacgc agcgaggcgt tgccgtcggt gacgaccacc
     3001 gcccccggtt tcaaaccgtt gaggatttcc atcgcgccgt ccccggcccg caggccgacc
     3061 gtcacttcgc gtcgccgggc cacgttcccc tccgcctcga acacgacgaa gccgttctcg
     3121 gtcgcgagga cggcccgctc ggggacgagc agggcgccgg cgcggcgctc ggtcgcggcg
     3181 cgcacctcgg cgaagaaccc gggcttgagg accgcctcgc gatcatcgag gagcgccagg
     3241 cactcgacca tgcggctgcc gggatcggcg ctgcgggaga cgtggtagag gcgggcctcg
     3301 aaggtgcggc cgggccaggc ggcggtcgtg aaggtcaccg ggagcccgtc cttgaggcgg
     3361 atcgattcgc tctcggtcac cctgaagcgc agccgcaggc ggcgggtgtc gacgagggtc
     3421 gcgagcacga cgtcgctgtc gacgtgctgc ccggtgacca ccgacttgcg gttgatgatg
     3481 ccggg
//
LOCUS       DASRIQ010000652         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_510189, whole genome shotgun sequence.
ACCESSION   DASRIQ010000652 DASRIQ010000000
VERSION     DASRIQ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510189"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFD06_16145"
     CDS             complement(<1..630)
                     /locus_tag="VFD06_16145"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZN05117.1"
                     /translation="MIRAGVAGWDYADWEGLVYPRPAPKGFDRLRFLAEHLDAIELNV
                     TFYRQPDAPAASSWARRVADRPGFRFTAKLFRDLTHVEPGLVGSEDPARLKEQERMAG
                     VYRAGIAPLADAGVLGAVLVQFPHRFHDRPESRRHLERLAGLFPGLPLVAELRHRSWG
                     HEEALQFLHRLGVGFCNIDQPALPTTLRPTGHVTSRVAYIRLHGRNAAAW"
     gene            863..1375
                     /locus_tag="VFD06_16150"
     CDS             863..1375
                     /locus_tag="VFD06_16150"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HZN05118.1"
                     /translation="MQFKIGDKVVYPNHGLGVIEQIEKRTIGDRMEEFLRLRIVGNDS
                     MVMVPRGNTANVGLRPIVSRKEIESVFDVLKDTRIKVFDDWKGRFQENSDKMRTGSIT
                     EVAMVFKSLTHLSLQKNLSYRERRMLDRARYLIVSEIAEVEKMKTEDVEIKVDKAVAS
                     ASGPRTVRDR"
     gene            1465..>2266
                     /locus_tag="VFD06_16155"
     CDS             1465..>2266
                     /locus_tag="VFD06_16155"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HZN05119.1"
                     /translation="MDAGLLRAIGEEIGRRLTEQEVQRVSDLGSGRFLLRFATPDRDN
                     LLISVRPDLPRLYLMPRGGGHREIPHGPFAAALDRDLTGARLETIRIPPGERIADLGF
                     RRADSEGARVTRRLVAELFGRAASAHLLDAAGIVLATTRRGEARGWSAATGEPYRPPA
                     ARPTLQEASTDAEAGTLPGSDPPVFEVHSVRPLEELTEEVPIGRDDLVLAGGPGREPP
                     PAAATGRPRHVTRFTSPSEAAAAVFERLERRRDFEAERARHQARARKEV"
BASE COUNT          368 a          766 c          812 g          320 t
ORIGIN      
        1 ccaggcggcg gcattgcggc cgtgcagacg gatgtaggcg acccgggacg tcacgtgtcc
       61 cgtcggccgg agcgtcgtcg gcagggccgg ctggtcgatg ttgcagaagc cgaccccgag
      121 acggtgcagg aactgcagcg cctcctcgtg tccccaggat cggtgccgca gctccgccac
      181 cagcggcaga ccgggaaaga gcccggcgag ccgctcgagg tgccgccggc tctccgggcg
      241 atcgtggaag cggtgcggga actggacgag gaccgcgccg agcaccccgg cgtcggccag
      301 cggagcgatc ccggcgcggt acactccggc catccgctcc tgctctttca gccgcgcggg
      361 gtcctcgctt ccgaccagtc cgggctcgac gtgggtgagg tcgcggaaga gcttggcggt
      421 gaaccggaag ccgggacgat ccgcgacccg gcgcgcccag gaggaggccg cgggggcgtc
      481 cggctggcga taaaaggtga cgttcagctc gatagcatcc aggtgttcgg ccaggaaacg
      541 caggcggtcg aaccctttcg gtgccggacg cgggtagacg agcccctccc agtccgcgta
      601 gtcccaaccg gccacgcccg cacgaatcat gggaggctcc tggagtcatc ttaaccggcc
      661 cggggcctcc aaaacggccg caaacggccg attcgggggc ccggagtcag atcgtcgaga
      721 cgccggtccc ctgccaaacc cgcgccaaag gcccgcaacc attgatctcc gagggggttc
      781 ctgataaaat cacccgacgc acagcgcccg gccgggggcg tcccaatcgc gacgaaacca
      841 ccggaccggg gggaggctgc ctttgcaatt caagatcggc gacaaggtcg tgtacccgaa
      901 ccatggtctg ggggtcatcg agcagatcga aaagcgcacg ataggggatc gcatggaaga
      961 gttcctgcgc ctgcgcatcg tgggcaacga cagcatggtg atggtccctc gcggcaacac
     1021 ggccaacgtc ggccttcggc cgatcgtgtc ccgcaaggag atcgaatccg tgttcgacgt
     1081 tctcaaggac acccgcatca aggtcttcga tgactggaag gggcgtttcc aggagaactc
     1141 cgacaagatg cgcaccggct ccatcaccga ggtggcgatg gtgttcaaga gcctgacgca
     1201 cctgtccctg cagaagaacc tgtcgtaccg ggagcgtcgc atgctcgatc gcgcccggta
     1261 cctgattgtc agcgagatcg ccgaagtcga gaagatgaag acggaggacg tcgaaatcaa
     1321 agtcgacaag gcggtcgcct ccgcttccgg cccgcgcacg gtgcgcgatc ggtaaacggt
     1381 ccgaatcgat agaatcccgg cccggcggcg cgagccgccg ggcttttttt tgagacgcgc
     1441 aggcgcgcgg gcgaggcttc tggcttggat gccggactgc tgcgggcgat cggagaggag
     1501 atcgggcgcc gcctgacgga gcaggaggtg cagcgcgtct ccgacctggg gtccggccgc
     1561 ttcctgctgc gcttcgccac cccggaccgc gacaacctgc tgatttccgt gcgccccgac
     1621 ctgccgcgcc tgtacctcat gccgcgtggg gggggacatc gcgaaatacc gcacggtccg
     1681 ttcgccgcgg cgctggatcg cgatctcacc ggtgcccggc tcgagacgat ccggatccct
     1741 ccgggcgagc ggatcgccga cttgggattc cggcgggccg actccgaagg cgcccgtgtg
     1801 acgcggcgtc tcgtggcgga gctgtttggc cgcgccgcgt ccgcccacct gctcgacgcc
     1861 gctggcatcg tgctcgccac gacccgccgc ggggaggcgc gcggatggtc ggcggcgacg
     1921 ggggagccgt accggccccc ggcggcgcga ccgacgctcc aggaggcgtc gaccgatgcg
     1981 gaggcgggaa ccctcccggg gagcgatcct cctgtcttcg aagtgcactc cgtccggccg
     2041 ctcgaggagc tgaccgagga ggtgccgatc ggccgcgacg atctcgtcct cgcgggcggt
     2101 ccgggccgcg agccgccccc cgccgcggcc accggccgtc cgcggcacgt cacccggttc
     2161 acgagtcctt ccgaggcggc cgcggcggtg ttcgagcgtc tggagcgccg gcgcgatttc
     2221 gaggcggagc gggcgcggca ccaggcgcgg gcgcgcaagg aggtgg
//
LOCUS       DASRIQ010000653         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_512827, whole genome shotgun sequence.
ACCESSION   DASRIQ010000653 DASRIQ010000000
VERSION     DASRIQ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512827"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VFD06_16160"
     CDS             <1..1191
                     /locus_tag="VFD06_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05120.1"
                     /translation="YAEERLGMSRSRAGERARLARALRTLPAVAAACASGRIAMEAAA
                     LIHRILVDADGNAALEADGFAALQATWVEHASAVTIKRMRDEARAAGRYRVRPRPSAD
                     AADGRQHPAVPPSPLSDAAWHRSIRRESGTATRRILAFGRLAAGFDGHVAAGPGAGMA
                     AAAAAAKAAAEPDVFHSEPAALRSEPDVFHSRLHEPDVFLRLRLPADMAAGLIATIAA
                     ARDIVEARVGEVPWDQHWPPEPSEDTGAATADASPPPSEWAARVAFVRGRRAPMWVGL
                     LALLEDFTATWDTDDAAPARRDDRIFVRDGWRCAAPGCSSRRHLEDHHIVYRSRGGGH
                     DETNRVSLCCFHHQRGEHGGLMQVRGSAPTGLTWRLGRDDVAVAYRNERRLCDMSNQT
                     LGDA"
     gene            1671..>2840
                     /locus_tag="VFD06_16165"
     CDS             1671..>2840
                     /locus_tag="VFD06_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05121.1"
                     /translation="MKTLRAWIAMGYRWVTVAGVAGAPVMVVIAAAVLSIAPASAAPG
                     RSLTFEERVAAQKAIEQVYWNHRIWPKENPGRKPSLSEVLPDSTIRARVDDYLLKSSA
                     LEKLWQHPITPAQLQNELNRMAADTRAPEILRELFAALGNDPHLIAETLARQTLADRL
                     VRNWYANDERFHGKLKGKAEAALAACLEVGCMRSLGGDYRETTWRLRSGRDEAPANDP
                     RHGMVRLDAGEWQSHLDGLVARLGGAPGSLPALRLSGLEETPEAFVVTAVLGQGKEEV
                     RTATVTWRKTSFDDWWKLGRKALVARIEDNSRFFTLPVISSSGCTADTWSLTSNAIPS
                     ARIGHTAVWTGTEMIIWGGSLDIGGRYDPASETWKPTSTGANLPQVRREHTAVWTG"
BASE COUNT          430 a         1000 c          980 g          430 t
ORIGIN      
        1 tatgccgagg agcggctggg catgtcccgc agccgcgccg gcgagcgcgc ccgcctggcg
       61 cgagccctgc gcaccctccc cgctgtcgcc gcggcctgcg cttccgggcg cattgccatg
      121 gaagcggcgg ccctcatcca ccgcatcctg gttgacgccg acggtaacgc ggcgctcgaa
      181 gccgatgggt tcgccgcgct gcaggccacc tgggtcgagc acgcctccgc cgtcactata
      241 aagaggatgc gggacgaggc gcgcgccgcc ggacgctatc gtgtccggcc gcgtccctcc
      301 gccgatgcgg ccgacgggcg gcagcatccc gccgtgccgc cttccccgct ctccgacgcc
      361 gcctggcacc gctccatccg ccgcgagtcc ggcaccgcga cccgccgcat tcttgcgttc
      421 ggtcgcctgg ccgccggctt cgatggccac gtcgctgccg gaccgggcgc cggaatggcc
      481 gccgccgcgg ctgcggcgaa ggcggccgcc gagccggatg ttttccattc cgagccggct
      541 gctctccgct ccgagccgga tgttttccac tcgcgactgc acgagccgga cgttttcctc
      601 cggctgcgcc tcccggccga catggccgcc gggttgatcg cgacgatcgc ggccgcgcgc
      661 gacatcgtcg aggcgagagt cggcgaggtc ccgtgggacc agcactggcc gccggagccg
      721 tccgaagaca ccggcgcggc cacggcagac gcttcgccgc ccccctcgga atgggcggcc
      781 cgcgtcgcct tcgtgcgcgg gcggcgggcg ccgatgtggg tcggcctgct cgcgctcctc
      841 gaggacttca cggcgacctg ggacaccgac gacgccgcgc cggcgcgccg cgacgatcgc
      901 atcttcgtcc gcgatggttg gcgctgcgcc gctcccggct gctcctcccg ccgccatctc
      961 gaagaccatc acatcgtcta ccgctcccgc ggcggggggc acgacgaaac gaaccgggtc
     1021 tcgctgtgct gcttccacca ccagcgcggg gagcacggcg gcctcatgca ggttcgcggc
     1081 agcgcgccga ccggcctcac ctggcggctc ggccgcgacg acgtcgcggt cgcctatcgc
     1141 aacgagcgtc gcctgtgcga catgtccaac cagaccctcg gcgacgcatg agtggcgggg
     1201 atgcgggaag tcacgggctt gccgggcgtc cgggacgact cggggccacg gccggcccgg
     1261 atggcgaggc tcgggaacga ggcggcgtgc gttcgattcc ggcccagctc cttggcccgg
     1321 tcgtcggcgc agcaggcctc catgtccggc cctccttccg gatgagtgcc cggggacacg
     1381 gacggggaac gccaccagtc gaacagcgcc cccctccggc cgggccgagg atcatgcatc
     1441 cgccggcatc ttcatatggg attgcgattg ccgccgcgct caatgaggcg ctcatctgcg
     1501 gggtagaacg tgctctcgtc ctctgcagcg cggaggtgga tttcggccaa gcctccgaac
     1561 caagatcgcc gtgagccgac cttgtggtgt gccgcgatct gacgtgttgc tgcgaagcgg
     1621 cttgatcctc ccgtccttgt accgttagag agtagggaag gggatcgaca atgaagactc
     1681 tccgggcatg gatcgcgatg ggatacaggt gggtgacggt cgcgggggtc gccggcgccc
     1741 ctgtcatggt cgtgatcgcc gccgccgttc tctcgatcgc ccctgcatcg gccgcgccgg
     1801 ggcgcagcct gaccttcgag gagcgcgtcg ccgcccagaa ggcgatcgag caggtctact
     1861 ggaaccaccg catctggccg aaggagaatc ccggtcggaa gccgtcgctc tcggaggtgt
     1921 tgccggattc gacaatacgc gcccgagtcg acgactatct cctgaagtcg agcgccctcg
     1981 aaaaactctg gcagcatccc atcactccgg cccagctcca gaacgagctg aacagaatgg
     2041 cggccgatac gcgcgctccg gagatcctcc gcgagctgtt cgcggctctc ggaaacgacc
     2101 cccacctgat cgccgagacg ctggcccgcc agaccctggc tgaccggctg gttcgcaact
     2161 ggtacgcgaa cgatgagcgc ttccacggaa agctcaaggg gaaggcggag gccgcgctgg
     2221 ccgcctgtct ggaggtcggc tgcatgcgat cgctgggggg agactaccgc gagacgacct
     2281 ggaggcttcg aagcggacgg gacgaggcgc cggcgaacga tccgcgccac ggaatggtgc
     2341 gcctcgatgc aggggaatgg cagtcccacc tggacggcct ggtcgcgagg ctcggcggcg
     2401 caccggggtc tcttccagcg ctccgcctca gtggactcga ggaaacgccg gaggcgttcg
     2461 tcgtcacggc ggtgctcggg caaggcaagg aggaggtcag gacggcgacc gtgacgtggc
     2521 ggaaaacctc cttcgatgac tggtggaagc tcggacgaaa agcacttgtt gctcgaatcg
     2581 aggacaactc tcgattcttc acgctccccg tgatctcgtc ctcggggtgc accgccgaca
     2641 catggagtct cacgtcgaat gcgattccgt cggcgcgtat cggccacacg gccgtgtgga
     2701 caggcacgga gatgatcatc tggggcggca gcctggacat tggaggccga tacgatcccg
     2761 ccagcgagac gtggaagccg acctcgaccg gggcgaacct cccccaggtt cgtcgtgaac
     2821 acacggccgt atggacgggc
//
LOCUS       DASRIQ010000654         3770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolia bacterium isolate
            SMAG_U10114 k141_513772, whole genome shotgun sequence.
ACCESSION   DASRIQ010000654 DASRIQ010000000
VERSION     DASRIQ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963642
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolia bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolia bacterium
            Bacteria; Acidobacteriota; Candidatus Polarisedimenticolia.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:24:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,207
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 21 of 25
            Pseudo Genes (internal stop)      :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Candidatus Polarisedimenticolia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513772"
                     /isolate="SMAG_U10114"
                     /isolation_source="soil"
                     /db_xref="taxon:3069555"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFD06_16170"
     CDS             complement(<1..373)
                     /locus_tag="VFD06_16170"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZN05122.1"
                     /translation="MIVIGLEAPAFAVTPTVTAFTPTAAPADCVLVITGTNFNNPTAS
                     NVTIGGDATGDFRVVSDTEIWAEVPGGAINGQVSVTNATGTGSLDGFIDADDGGGCVP
                     TVTSFTPTCGPGNPAGTGKTEV"
     gene            complement(840..1559)
                     /locus_tag="VFD06_16175"
     CDS             complement(840..1559)
                     /locus_tag="VFD06_16175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN05123.1"
                     /translation="MATTAGSSEARSRGHARSRLGVVVVDPLPVVRAGLGMLIEDQPD
                     MRVLAEAGSADECLAALRRIRRSQLVVLVGLGLPGARDSLWLIGTLRERSAQTTILAS
                     GAGADATTISRGLVVGADGYLDKDVDPAQYLQAIRRAAGGEFVLIGTRVNGVGFLADG
                     YDGRRHIESLLTRREQEVLKVAAQDMTARDIARHLGVRERTVTTHLGRIYGKLGVNSR
                     VGAVIAAARSGLVAVGSPETE"
     gene            complement(1611..3074)
                     /locus_tag="VFD06_16180"
     CDS             complement(1611..3074)
                     /locus_tag="VFD06_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05124.1"
                     /translation="MSSATQAPSSSGPAEPPVPQTRVRSEVAPAARGSSPTAILTRGW
                     TTGLARGGLAFAVMVALGQAVAFAVNLGQGLRMSAGTAAKLGWLYVGWFHHATITTHL
                     TPPVEPGPAGSAPTGGLTADVGLAMMLGTFLAIALLYRGGRVVADRAGGGAGARVLHG
                     MKVAPAYAILPFLVTGLVTIDIPIPRNALFTGSLQMRPWDLSWLVLPLLIAVAAGAAG
                     GLRSGRHELVSHDPWGRRAAGATAGGVRMFVLGLALSLVGVLVLSAIRPGATRTYLET
                     VSGPPPEQAAVVAANQLLLLPNQSMWVLVPAMGGCDGLSGGSVSATFLCYSKVPTRVS
                     VNPEGLTSGTPVFRAGFTKATSAYLLFLLVPALSVVLGGRHAARKRARLKWEAVGVGA
                     ASGLVFAVLVGLGSWFASVSAGLSSPAAGIPANASVMIGPGVLAAGLLALAWGVVGGA
                     LGGWLGGRRFPPRALLPEPSGLLTRRGTARPGSDPRG"
     gene            complement(3071..3382)
                     /locus_tag="VFD06_16185"
     CDS             complement(3071..3382)
                     /locus_tag="VFD06_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN05125.1"
                     /translation="MSLRLDIATIAKIHSDRIYDLILPEREDQETVTVPLPFLENVRS
                     TGQKLEGFAPGYITRALERLEGTAKIQRVRRKAVIPRESLAFVAAAFRKAAESAERSH
                     P"
BASE COUNT          604 a         1383 c         1215 g          568 t
ORIGIN      
        1 tcacctccgt tttgccggtt ccggccgggt tgcccggtcc gcaggtcggc gtgaacgatg
       61 taacggtcgg aacgcagccg cccccatcgt cggcgtcgat gaagccgtcg aggctccccg
      121 tgccggtggc attggtgaca ctgacctgcc catttatcgc gccgccggga acctccgccc
      181 agatctcggt atctgagacg acgcggaagt cgcccgtggc gtcccctccg atcgtgacgt
      241 tcgacgccgt cgggttgttg aagttcgtcc ccgtgatcac cagcacgcag tctgccgggg
      301 cagcggtcgg cgtgaacgcc gtgaccgtcg gggtcaccgc gaacgccgga gcttcgaggc
      361 cgatcacgat catggccact ccgacggcga cccctgctac gcggcggccg atgtgccgct
      421 tacctgatct catacgtctg tcctcacttt ccgcatcgac atcttccttc ttcgccttct
      481 ctaccgagcg ctacttcctc cttcccttct cgatcccctc ctccccttct cgatcccctc
      541 ctcgcggggc acgcagacga gaccctcaag aggtggggaa gtgggccgac tctaaactcc
      601 gtttccgcgc gagtcaaact tcgcccttgt ccgcgccgga gcttcctgca ccaagaaacc
      661 cgctcacctc catgcacggc catgcacggc gcagcactct agagaacgac tttctacaga
      721 gaacgacttt ctacggcgaa ggttgcgagc cgaggcgtcg ggagttgtgc ctacgacgaa
      781 gacaccaccc cgccgaccac tcctagacct tgcggcgcag cgtcaggtcg acgggatcct
      841 cactccgtct ccggggagcc aaccgcgacg agacccgatc gcgccgccgc gatcactgcg
      901 cctacccggc tgttgacgcc gagcttgccg tagatgcggc ccaggtgtgt cgtcacggtc
      961 ctctcacgaa cgccgagatg tcgcgcgatg tcccgagccg tcatgtcctg ggctgcaacc
     1021 ttcaacacct cttgctcgcg tcgggtcaga agagactcga tgtgccgcct cccgtcgtag
     1081 ccgtcggcga ggaagcccac cccgttgacc cgcgtgccga tcagaacgaa ctcgccccca
     1141 gcggcccggc ggatcgcctg gaggtactgc gcgggatcga cgtccttgtc taggtaccca
     1201 tcggccccca cgaccagccc gcgcgagatg gtcgtggcat cggcgccggc ccccgacgcc
     1261 aggatcgtcg tttgcgcgga gcgctcgcgt agggtcccga tcagccagag cgaatcgcgc
     1321 gctccgggga gacccagccc cacgaggacg accagctgcg agcgccggat cctcctgagg
     1381 gccgcgaggc actcgtcggc agagcccgcc tcggcaagga cccgcatgtc cggctggtcc
     1441 tcgatcagca tccccaggcc ggcccgcacc acggggagcg gatccacgac cacgacaccc
     1501 agacgcgagc gcgcgtgccc ccggctcctc gcctctgagg atcccgcggt cgtggccatc
     1561 tccctgatat atcggcgatc ccttcgcgga cttgcgccgt cgcgtcctga tcagccgcgg
     1621 ggatccgagc ccggccgggc ggttcccctc cgcgtgagga ggcccgaggg ctcggggagc
     1681 agtgccctgg gtgggaaccg tctgcccccc agccatcccc cgagcgcgcc tccgacgacc
     1741 ccccacgcga gcgcgaggag cccggcggcc agcacaccgg gaccgatcat gaccgaggcg
     1801 ttcgcgggga tgcccgcggc tggcgaggag agccccgccg ataccgaggc gaaccacgag
     1861 cccaacccga ccaggaccgc gaagaccagg ccggacgcgg ctcccacgcc gacggcctcc
     1921 cacttgagtc gggcgcgctt cctggcggca tgtcggcctc ccagcaccac cgacagcgcg
     1981 ggaaccaaga ggaacaggag gtaggcggat gtcgccttcg tgaagccggc ccggaagacc
     2041 ggcgtgccgg aggtcaggcc ctcggggttc accgagaccc tcgtcgggac cttcgagtag
     2101 cagaggaagg tcgcggagac gctgcccccg gaaagaccgt cgcagccacc catcgccggg
     2161 accagcaccc acatcgactg gttcggcagg agcaacaact ggttcgcggc gaccacggcc
     2221 gcctgttcgg ggggtggccc ggagaccgtc tcgagatagg tcctggtcgc gccgggtcgg
     2281 atcgcgctta gcacgagcac cccgaccaac gagagcgcca ggccgagaac gaacatccga
     2341 acccctccgg cggtcgcgcc cgcggcccgg cgaccccagg gatcgtgcga gaccagttcg
     2401 tggcgccccg agcgcaggcc cccggcggcc cccgctgcca cggcgatcag tagggggagc
     2461 acgagccacg agaggtccca cggtcgcatc tggaggctgc ccgtgaacaa cgcgttccgc
     2521 gggatgggga tgtcgatggt cacgagcccg gtaaccaaga atggaaggat cgcgtacgcg
     2581 ggcgccacct tcatgccgtg aaggactcgg gccccggcac cgccccccgc ccggtccgcc
     2641 acgacgcggc ctcccctgta caggagcgcg atcgcgagga acgtgccgag catcatcgcg
     2701 agccccacgt cggccgtgag gccgccggtc ggcgccgagc ccgcgggccc tggttcgacc
     2761 gggggggtca ggtgcgtcgt gatcgtcgcg tggtggaacc acccgacgta gagccagccc
     2821 agcttggcgg ccgtcccggc cgacatgcgg agcccctggc cgaggttcac ggcgaacgcc
     2881 accgcctgcc ccagggcgac catcaccgcg aaggccagcc caccccgggc cagccccgtg
     2941 gtccaccccc gggtgaggat ggcggtcggc gaagacccgc gcgccgccgg ggcgacctcg
     3001 gatcggacgc gtgtctgcgg aacggggggt tccgccggac ccgatgatga tggcgcctgc
     3061 gtcgccgaac tcacggatgg ctcctctccg ccgactccgc cgccttccgg aacgcggccg
     3121 cgacgaaggc aagactctcc ctcgggatga cggctttcct cctgacccgt tggatcttcg
     3181 ccgtcccctc gagcctttcc agcgctctcg tgatgtagcc gggcgcgaag ccttccagct
     3241 tctgccccgt gctccgtacg ttctcgagga acggaagagg cacggtcacc gtctcctggt
     3301 cttccctctc cgggaggatc aggtcgtaga tccgatccga gtggatcttg gcgatcgtgg
     3361 cgatgtccaa gcgcaagctc accgggcgct ccctccctgt tcctctgttc ctctacgaag
     3421 acctccatta ctctcggcgc gatggccgcc caacctgata cgagaggggc tcttttccct
     3481 tcggtccgcg agcatcggga cgggaggagg aagagttcga caccgcaccc ggccgcccgc
     3541 tgccaacggc agggagatag agtgcgcgat cggcgctgct cggagtcgtt cccgtagccg
     3601 tcgatcgact cgcccgtgaa cggtccgcgg tgtaggccgg ccgggtgacc gtagcgtcag
     3661 acccggcgga cgcatctgga ccgataaatg gtccttcggg gggatacgcg cgctccaacc
     3721 tcgtgccgat attcaccgtc gaacccaacc ttggggggaa cgatgtcggg
//
